LOCUS       DASWHU010000001        10940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2587914, whole genome shotgun sequence.
ACCESSION   DASWHU010000001 DASWHU010000000
VERSION     DASWHU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2587914"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VGB88_00005"
     CDS             complement(<1..635)
                     /locus_tag="VGB88_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX9645847.1"
                     /translation="MAKVRLDQLLVRRGLTETRARAQGLILAGKVFSGSRRLDKAGQQ
                     VAEDAPLEVRGPAHPWASRGGLKLVHALDHFAIDPKGAVCLDIGASAGGFTDVLLARG
                     AARVYAVDVGHGQLDWRLRNDPRVVVLERTNARTLTRAEVPEPVGLIVCDASFIGLAT
                     VLPAPLALAGDHARLIALIKPQFEVGPDKVGKGGVVRDQAARATACARVAD"
     gene            complement(650..1576)
                     /locus_tag="VGB88_00010"
     CDS             complement(650..1576)
                     /locus_tag="VGB88_00010"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX9645848.1"
                     /translation="MTRLREARFGGRRKARLDAALKAAATAVEAELAALIPNHDAPHA
                     RLLRAMRYAALGGGKRLRPFLTLESAKLFDADPAGSLRAACAVEMVHCYSLVHDDLPA
                     MDDDEMRRGRPTTHVEFDEATAILAGDALLTLAFETLAAPETHADAAVRAELVLVLAR
                     AAGMNGMVGGQMIDLEAEGTALDLAQITTLQAMKTGALITAACEAGAILGRADATARR
                     ALVAFAHDLGLAFQIADDLLDVEGEAARVGKGVGKDAAAGKATFVSALGIDQARARAL
                     SLAEAAACRLAGFGERAETLRSAARFVVERQH"
     gene            complement(1573..1866)
                     /locus_tag="VGB88_00015"
     CDS             complement(1573..1866)
                     /locus_tag="VGB88_00015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9645849.1"
                     /translation="MARKSDSSRAAPEDAFEDIARLSFEQALAELEDIVRKLEKGEID
                     LDGAIAAYARGAALKRHCEDKLRQAEARVSRINLAGDGSVRPEPFEPESGSTP"
     gene            complement(1927..2850)
                     /locus_tag="VGB88_00020"
     CDS             complement(1927..2850)
                     /locus_tag="VGB88_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX9645850.1"
                     /translation="MGVLLYTHDACLRHDTGPGHPERAARLEAVLAALAAPRFAGLDR
                     RQAPAASLDQIALVHPRDWAERLLAAAPRAGYVALDADTVMSPASGEAALRAAGAVCA
                     GVDAVIAGEGARAFCAVRPPGHHAGPRRAMGFCLFNNVAIAAAHALRAHGLMRVAIAD
                     FDVHHGNGTQAAFYAEARVLYGSSHQMPLYPGTGAASETGVGNIVNMPLDPGSGGTEF
                     RAAWRHSFLPRLEAFHPELILVSAGFDAHRDDPLAALELDEDDFAWITAELVRVAEHS
                     CAGRVVSTLEGGYDLDALAASTAAHVGALGA"
     gene            2991..4151
                     /locus_tag="VGB88_00025"
     CDS             2991..4151
                     /locus_tag="VGB88_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9645851.1"
                     /translation="MTVAKGKSAASTVIQLVVVGAIGIGAYWAWQNRESLPFIGGAAE
                     QAAAPARPPTPVDVALAREGSVVVTLEALGTARSNEAVTVMSDVMGIVSEVRFDEGTR
                     VKAGEVLVRFDSTIASAEVAVRQAEIEVRHAQLENARQLYDRAKQLMATQNVPAARFD
                     ELAAQLKGAEAEVASAEARLQAARAALAKRQVQAPFAGRLGIRKISVGALVEPGDAII
                     TLDDISVIKLDFQIPERNLSYIAPGQEITAHTDAYPDRVYFGQVSSVDSRVDPVTRAV
                     AVRALIDNPDESLKPGMFLLVELGVATRNHAVIIPEEAVVADGTARFVFVVAGDTVKQ
                     VPVDLGQRMPGEVEVLTGVEAGQKVVVGGVQKVRDGAAVAVREQATEAANAE"
     gene            4198..7344
                     /locus_tag="VGB88_00030"
     CDS             4198..7344
                     /locus_tag="VGB88_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9645852.1"
                     /translation="MVLSDVSIKRPVFATVVSLVLVIFGLFAFGRLAIREYPDIDAPV
                     VSVIALYKGASAQIIESQVTQIIEEAVAGIEGIKKITSKSREERSQVDIEFILSRDVD
                     AAANDVRDKVSAVIGNLPLDVETPIVNKVEADASPMLWIALTSAEWNAIELSDYADRY
                     LVDRLSVVPGVASVIIGGERRPAMRIWLDRRAMAARQITVADVERALLQQNVELPSGR
                     IESTQREFTVRTESGLVTPEQFRDLVVFEKDGYLVRLDEIADVELGTEELRYEVRANG
                     EAAIGLGVVKQSKANELEIANGIRAEIERLRPSLASQVKMWVAYDKSRFVQASIDEVF
                     HALFIALGLVVGVIFLFLRNLRATLVPAIAIPVSLIASFMVLAALGYSINVLTLLAYV
                     LAVGLVVDDAIVVLENIHRRMENGEPVLLASVRGARQIGFAIIATTLALVAVFVPISL
                     MSGNTGRLFSEFGVTVAAAVLFSGLVALTLTPMMCSKLLVDHEHESWFYRITEYFFRA
                     LNAGYSLTLRGALKVPVIMIALGIALSVIAKGFYDNINQEFAPTEDRGVFLVVLNAPE
                     GATIDYTRAYLEEAERKIQPLVEADEVETVFGVVAPGLSRPSPVNFAIAFVVLKPWRE
                     RARSQQEIVAQMFPQLLSIPGAMVFAINPPSLNQPGRQAPVQYVIGGPSYDTLREWSD
                     IMVEGAQENPKLLSVQTDYLETKPELRVDIDRNRAADLGVSIESVGRSLEAMLGSRYV
                     TTFNDRGVQYNVVLQARDSDRVTPNDLNNIYVRSSSTGQLIPLSNLVTLNEAAGPKEL
                     NRFDRMRSVTISASLAPDYTLGEALDYMDGLGRARLPLEARITYDGQSREFRESSSSL
                     ALTFLLAVVIIYLVLGAQFESFIHPFIILLSVPLAVTGALGTLLLMGITLNVYSQIGM
                     VMLIGLVAKNAILIVEFSNQLREQGADIREAVMRASMARFRPILMTTISTVCGAIPLA
                     LATGAGAESRASIGWVVIGGVSFSTVLSLFIVPVLYLLLARFTKPSSHVADRLRKLEA
                     EHATGAYGEVAARPAPAE"
     gene            7446..8492
                     /locus_tag="VGB88_00035"
     CDS             7446..8492
                     /locus_tag="VGB88_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M29"
                     /protein_id="HEX9645853.1"
                     /translation="MLTDRIEAKWIDAFRRTLALCKVARGEAVAILSETQSRALNVHL
                     AELALLALGARPFHVVLPTPPQTAPVPVRSTGASQVVRGLAPVVGALAQSGLVVDVTV
                     EGMLHAPELPDILAGGARLLMVSNEHPDVLERLAPAPELAPKVKAGIRMLKEAQLMRI
                     ESAAGTDLVVRLAGARAGGVWGWCDKPGQVAHWPGGLCLCFPAARSVSGRIVLDRGDV
                     NLTFKRYLESPVTLTVEDDYVTAIGGDGLDAELMRSYFAAWGERDAYAVSHAGWGMNP
                     AARWDAMTMYDRRDINGTELRAFAGNFLYSTGANEVAGRFTAGHFDLPMRGCTVRLDD
                     RVVVDAGRLQGELA"
     gene            8489..9322
                     /locus_tag="VGB88_00040"
     CDS             8489..9322
                     /locus_tag="VGB88_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9645854.1"
                     /translation="MSALGDMLTPAYRLVGDGPETLLYLHGLGGDRTSFDGQIAARPR
                     PYRHLAWDMPGYGDSAPLAEMTFPALARAALRLLDDVGAARAHVVGHSLGGMIAQELV
                     HACPERIASLVLYATSAAFGGAGGDWQQRFLDERLAPLERGLGLRDLAPGLVAGLVAE
                     DADPAGVRAAIATMGAVPAAAYRAALTCLVSFDRRAELDAIRVPTLLIAGTRDTVAPA
                     EMMARMAARIASARLVRIEGAGHLAHLERPDAFNAALGGFLDELVARGRKDDDGLRAH
                     R"
     gene            9300..10514
                     /locus_tag="VGB88_00045"
     CDS             9300..10514
                     /locus_tag="VGB88_00045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9645855.1"
                     /translation="MGFAPTARQRELVELAARLGREKFAPRAARYDAEASFPFENYED
                     LRAAGLLALCVPEAYGGLGADFATYCLVSAELGRHCGATALTYNMHACTMLWSGPMTD
                     DLDMTNAERAQHAERQALHFRRVVKDGAIYAQPFSEGNPSAAGRAPFGTLARRSGGGW
                     VLNGKKIFASLAGAAHYYGILCTEDVDGASRKDTIYLAVPADADGFTVTGPWDPLGMR
                     GTVSRTLVLKDVFVADQAELMPRGVYYQAANRWPHMFLTLAPTYLGIAQAAYDFTVSY
                     LRGEVEGTPPVKRRMYPTKQIAVAEMHVKLEQTWAIFLRVISEARVDPDKAARLRLLA
                     AQYTIMENANDLCRLAIRTCGGQSMLRSLPLERLYRDSRCGSLMLPLTAELCLDRLGR
                     DALYEPGETDEA"
     gene            complement(10518..>10940)
                     /locus_tag="VGB88_00050"
     CDS             complement(10518..>10940)
                     /locus_tag="VGB88_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9645856.1"
                     /translation="WSRTLKYARRSELVLVAEDPDAGIVGFASCASAKGLDLPYRGEV
                     TMLYVHPDFQAQAIGRALLARSFGVLAGKGLGSALIWVLADNPARFFYQAMGGRLIAE
                     RRERMWGVPLQELAYGWSDLKAAIPRIAGGAIAKRHSG"
BASE COUNT         1543 a         3932 c         3876 g         1589 t
ORIGIN      
        1 cagtcggcga cgcgcgcaca cgccgtggcg cgcgcggcct ggtcgcgcac cacgccgccc
       61 ttgccgacct tgtcgggacc gacctcgaac tgcggcttga tcagggcgat cagccgcgca
      121 tggtcgccgg cgagcgcgag cggcgccggc aggaccgtgg ccagcccgat gaaacttgcg
      181 tcgcaaacga tcagcccgac aggctccggc acctcggcgc gggtgagagt gcgcgcgttg
      241 gtgcgctcga gcacgacgac gcggggatcg ttccgcagcc gccagtcgag ctggccgtgg
      301 cccacgtcga ccgcgtagac gcgcgccgcg ccgcgggcga gcagcacgtc ggtgaagcca
      361 ccagcggacg cgccgatgtc gaggcagacg gcgcctttcg ggtcgatggc gaaatggtcg
      421 agcgcgtgga caagcttgag cccgccgcgg ctcgcccagg gatgcgcggg cccgcgaacc
      481 tcgagcggcg cgtcctcggc gacctgctgg ccggccttgt cgagccggcg gctccctgag
      541 aacaccttgc cggcgaggat caggccttgc gcgcgcgcgc gtgtttccgt taggccgcgt
      601 cggaccaaaa gctgatcgag gcggaccttg gccaccgccc cgcccggcgt cagtgttgcc
      661 gctcgaccac gaaccgtgcc gccgaccgca aggtttccgc gcgctcaccg aagccggcga
      721 gacggcaggc ggcggcctcg gcgagcgaca gcgcgcgcgc ccgcgcctgg tcgatgccga
      781 gcgccgagac gaaggtcgcc ttgccggcgg cggcgtcctt gcccacgccc ttgccgaccc
      841 gggccgcctc gccctcgacg tccaagaggt cgtcggcgat ctggaaggcg agcccgaggt
      901 cgtgggcgaa ggcgaccagc gcccggcgcg cggtcgcatc ggcgcggccg aggatcgcgc
      961 cagcctcgca tgcggccgtg atcagcgcgc cggtcttcat cgcctgaagc gtggtgatct
     1021 gggcaaggtc gagcgcggtg ccctcggcct cgaggtcgat catctggcca ccgaccatgc
     1081 cgttcatgcc ggccgcgcgc gcgagcacga gcacgagttc cgcgcgcacc gccgcgtccg
     1141 catgggtttc gggcgcggcg agagtctcga aagcaagcgt cagcagcgca tcgccggcca
     1201 ggatcgcggt cgcctcgtcg aactcgacat gggtggtcgg ccggccgcgg cgcatctcgt
     1261 cgtcgtccat cgccggcagg tcgtcgtgga ccagcgagta gcagtgcacc atctcgaccg
     1321 cgcacgccgc gcgcaagctg ccggccggat cggcgtcgaa cagcttggcg ctttcgagcg
     1381 tgaggaacgg ccgcagccgc ttgccgccgc cgagcgccgc gtagcgcatc gcgcgcagca
     1441 ggcgcgcgtg gggcgcgtcg tggttcggga tcagcgcggc cagctcggcc tcgaccgccg
     1501 tggcggcggc cttgagcgcg gcgtcgaggc gggccttcct gcgcccgccg aagcgggctt
     1561 cgcgaaggcg ggtcaaggag tcgatccgct ttcgggctcg aacggctcag gcctgaccga
     1621 tccgtcgccg gcgaggttga tgcggctgac ccgcgcttcc gcctggcgca gcttgtcctc
     1681 gcagtggcgc ttgagcgcgg cgccgcgcgc gtaggcggcg atcgccccgt cgaggtcgat
     1741 ctcgcccttc tcgagtttgc gcacgatgtc ctcgagctcg gccagcgcct gctcgaagct
     1801 cagccgcgcg atgtcctcga acgcgtcctc gggcgccgcc ctggagctgt cggattttcg
     1861 tgccatggtc ccgcgccggc agtctaggga tcgctcgccc cgccggcaag cgtgcccggc
     1921 ccgcgcctat gcgccgagcg cgccgacatg cgcggcggtc gaggcggcga gcgcgtcgag
     1981 gtcgtagccg ccttcaagcg tcgacaccac gcgcccggcg cagctgtgct cggcgacgcg
     2041 caccagctcg gcggtgatcc aggcgaagtc gtcctcgtcc agctcgagtg ccgccaaggg
     2101 gtcgtcgcgg tgcgcgtcga acccggccga gaccaggatc agctcgggat ggaacgcctc
     2161 cagccgcggc aggaagctgt gccgccaggc ggcgcggaac tcggtcccgc ccgagcccgg
     2221 gtcgagcggc atgttgacga tgttgccgac cccggtctcg gacgccgcgc cggtgccggg
     2281 atagagcggc atctggtggc tcgagccgta cagcacgcgc gcctcggcgt agaacgcggc
     2341 ctgggtgccg ttgccgtgat ggacgtcgaa atcggcgatc gccacccgca tcagcccgtg
     2401 cgcccggagc gcgtgggcgg cggcgatggc gacgttgttg aacaggcaga agcccatcgc
     2461 ccggcgcggc ccggcgtggt gcccgggcgg gcgcacggcg cagaacgcgc gcgcgccttc
     2521 gccggcgatc accgcatcga ccccggcgca caccgcgccc gccgcgcgca gggccgcctc
     2581 gcccgaggcc ggcgacatca ctgtgtccgc atccagcgcc acgtagcccg cccgcggcgc
     2641 tgccgcgagc agacgctcgg cccagtcgcg cgggtggacg agcgcgatct ggtcgaggct
     2701 cgccgccggc gcctggcgcc ggtcgaggcc ggcgaaccgg ggcgccgcca gggcggcgag
     2761 caccgcttca aggcgcgccg cacgctcggg atgtcctggg ccggtatcat gccggagaca
     2821 ggcgtcgtgg gtgtagagca gcacgcccat ggcgggcgcg accctagcag actccccagc
     2881 cacccggaac ggacagatca ggactgcgtg aacggccctt gttggcgaac ggctttatgg
     2941 gaataatccg cggctctgag cagtaggccc ggcgcgtccc gggacgaaag gtgactgtgg
     3001 ccaagggcaa atccgccgcc tcgacggtga tccagctggt cgtcgtcggc gccatcggca
     3061 tcggcgccta ttgggcgtgg cagaaccgcg agtcgctgcc gttcatcggc ggcgcggcgg
     3121 agcaggccgc ggcgccggcg cgcccgccga ccccggtcga cgtggccctc gcgcgcgagg
     3181 gctcggtggt ggtcaccctc gaggcgctcg gcaccgcgcg ctcgaacgag gcggtgaccg
     3241 tgatgtccga cgtcatgggc atcgtctcgg aggtgcgctt cgacgagggc acccgggtca
     3301 aggcgggcga ggtgctggtc cgcttcgaca gcacgatcgc gagcgccgag gtcgcggttc
     3361 gccaggccga gatcgaggtc cgccacgccc agctcgagaa cgcgcgccag ctctacgacc
     3421 gggccaagca gctgatggcg acccagaacg tgccggccgc gcgcttcgac gagctcgccg
     3481 cccagctcaa gggcgccgag gccgaggttg cctccgccga ggcgcggctg caggcggcgc
     3541 gcgcggccct tgccaagcgc caggtccagg cgccgttcgc cggccggctg ggcattcgca
     3601 agatcagcgt cggcgccctg gtcgagccgg gcgacgcgat catcaccctc gacgacatct
     3661 cggtgatcaa gctcgacttc cagatcccag agcgcaacct gtcctacatc gccccgggcc
     3721 aggagatcac ggcgcatacc gacgcctacc ccgaccgggt ctatttcggc caggtgtcct
     3781 cggtcgacag ccgggtcgac ccggtgaccc gcgcggtcgc cgtgcgggcg ctgatcgaca
     3841 accccgacga gtcgctcaag ccgggcatgt tcctgctggt cgagctcggc gtcgcgaccc
     3901 ggaaccacgc ggtgatcatc cccgaggagg cggtggtcgc cgacggcacc gcccggttcg
     3961 tgttcgtggt cgcgggcgac acggtcaagc aggtgccggt ggatctcggc cagcgcatgc
     4021 cgggcgaggt cgaggtgctg accggcgtcg aggccggcca gaaggtggtg gtcggcggcg
     4081 tgcagaaggt gcgcgacggc gccgccgtgg cggtgcgcga gcaggccacc gaggcggcca
     4141 acgcggagta gcgcccgcgc gcggcgcggg gtcacttagg gctcggagag acaggcgatg
     4201 gtcctttcgg acgtctcgat caagcgcccg gtgttcgcca ccgtggtcag cctggtgctg
     4261 gtgatcttcg gcctgttcgc gttcggccgc ctggcgatcc gcgaatatcc ggacatcgac
     4321 gcgccggtgg tgtcggtgat cgcgctgtat aagggcgcct cggcgcagat catcgaaagc
     4381 caggtcaccc agatcatcga ggaggcggtg gccggcatcg aggggatcaa gaagatcacc
     4441 tcgaagagcc gcgaggagcg ctcgcaggtc gacatcgagt tcatcctcag ccgcgacgtc
     4501 gacgccgccg ccaacgacgt gcgcgacaag gtctcggcgg tgatcggcaa tctgccgctc
     4561 gacgtcgaga cgccgatcgt caacaaggtc gaggccgacg ccagcccgat gctgtggatc
     4621 gcgctgacca gcgccgagtg gaacgcgatc gagctcagcg actatgccga ccgctacctg
     4681 gtcgaccgcc tgtcggtggt gccgggggtg gcgagcgtga tcatcggcgg cgagcggcgc
     4741 ccggcgatgc gcatctggct cgaccggcgg gcgatggcgg cgcgccagat cacggtcgcc
     4801 gacgtcgagc gggcgctgtt gcagcagaac gtcgagctgc cgtcggggcg gatcgagtcg
     4861 acccagcgcg agttcaccgt gcgcaccgag tccggcctgg tcacgcccga gcagttccgc
     4921 gacctggtgg tgttcgagaa ggacggctac ctggtccgcc tcgatgagat cgccgacgtc
     4981 gagctcggca ccgaggagct gcgctacgag gtgcgcgcca acggcgaggc ggcgatcggg
     5041 ctcggcgtgg tcaagcagtc gaaggccaac gagctcgaga tcgccaacgg catccgcgcc
     5101 gagatcgaga ggctcaggcc gagcctggcc agccaggtga agatgtgggt cgcctacgac
     5161 aagtcgcgct tcgtccaggc ctcgatcgac gaggtgttcc acgccctgtt catcgccctc
     5221 ggcctggtgg tcggagtgat cttcctgttc ctgcgcaacc tgcgcgcgac gctcgtgccg
     5281 gcgatcgcga tcccggtgtc gctgatcgcc tcgttcatgg tgctggcggc gctcggctac
     5341 tcgatcaacg tgctcacgct gctcgcctac gtgctggcgg tgggcctggt ggtcgacgac
     5401 gccatcgtcg tgctcgagaa catccaccgg cggatggaga acggcgagcc ggtcctgctc
     5461 gcctcggtgc gcggcgcacg ccagatcggc ttcgccatca tcgccaccac cttggcgctg
     5521 gtcgcagtgt tcgtgccgat ctcgctgatg tccggcaaca ccgggcgcct gttcagcgag
     5581 ttcggcgtca ccgtggccgc agcggtgctg ttctcgggcc tggtcgcgct gaccttgacg
     5641 ccgatgatgt gctcgaagct cttggtcgac cacgagcacg agagctggtt ctaccggatc
     5701 accgagtact tcttccgcgc gctcaacgcc ggctacagcc tgacgctgcg cggggcgctg
     5761 aaggtcccgg tgatcatgat cgcgcttggt atcgcgctgt cggtgatcgc caagggattc
     5821 tacgacaaca tcaaccagga gttcgccccg accgaggacc gcggcgtgtt cctggtggtg
     5881 ctgaacgcgc ccgagggagc gaccatcgac tacacgcgcg cctatctgga ggaggccgag
     5941 aggaagatcc agccgctggt cgaggccgac gaggtagaga ccgtgttcgg cgtcgtcgcg
     6001 cccggcctca gccggccgag cccggtcaac ttcgcgatcg cgttcgtggt gctgaagccg
     6061 tggcgcgagc gcgcgcgcag ccagcaagag atcgtcgccc agatgtttcc gcaactgctt
     6121 agcatcccgg gcgcgatggt gttcgcaatc aacccgccga gcctcaacca gccgggcagg
     6181 caggcgccgg tccagtacgt catcggcggg ccgtcctacg acacgctgcg cgagtggagc
     6241 gacatcatgg tcgagggcgc gcaggagaac ccgaagctgc tgagcgtgca gaccgactat
     6301 ctggagacca agccggagct cagggtcgac atcgaccgca accgcgccgc cgacctcggc
     6361 gtgtcgatcg agtcggtggg gcgctcgctc gaggcgatgc tcggctcgcg ctacgtcacc
     6421 accttcaacg accgcggcgt ccagtacaac gtggtcctgc aggcgcgcga ttccgaccgg
     6481 gtcacgccca acgacctcaa caacatctat gtccgctcga gcagcaccgg ccagctgatc
     6541 ccgctcagca acctggtgac cctgaacgag gcggcggggc cgaaggagct caaccgcttc
     6601 gaccgcatgc gctcggtgac catctccgcc tcgctcgccc ccgactacac gctcggcgag
     6661 gcgctcgact acatggacgg cctcgggcgc gcgcgcctgc cgctcgaggc gcgcatcacc
     6721 tacgacggcc agtcgcgcga gttccgcgag tcctcgagct cgctcgcgct caccttcctg
     6781 ctcgcggtgg taatcatcta tctggtgctc ggcgcccagt tcgagagctt catccacccg
     6841 ttcatcatcc tgttgtcggt gccgctcgcg gtcaccggcg cgctcgggac gctgttgctg
     6901 atgggcatca cgctcaacgt ctacagccag atcggcatgg tcatgctgat cggcctggtg
     6961 gccaagaacg cgatcctgat cgtcgagttc tcgaaccagc tgcgcgagca gggcgcggac
     7021 atccgcgagg cggtgatgcg ggcctcgatg gcgcgcttcc gcccgatcct gatgaccacc
     7081 atctcgaccg tgtgcggcgc gatcccgctg gcgctcgcca ccggcgccgg cgccgagagc
     7141 cgggcctcga tcggctgggt ggtgatcggc ggcgtcagct tctcgaccgt cttgagcctg
     7201 ttcatcgtgc cggtgctgta cctgctgctg gcgcgcttca ccaagccgtc gagccacgtc
     7261 gccgaccggc tgcgcaagct cgaggccgag cacgccaccg gcgcctacgg cgaggtcgcg
     7321 gcgcgccccg ccccggcgga gtagccgccc ctcccgatcc ccggcccgcg gcgtttgggc
     7381 ttgcgggcgg gtgattatct tctacgctct ctgcgctgtg acccgaggga gcggcgcgcc
     7441 cggcgatgct caccgaccgc atcgaagcga agtggatcga cgccttccgg cgcaccttgg
     7501 cgctgtgcaa ggtggcgcgg ggagaggcgg tggcgatcct gtccgagacc cagtcgcggg
     7561 cgctgaacgt gcatctggcc gagctcgccc tgctcgcgct cggcgcgcgc ccgttccacg
     7621 tcgtgctccc caccccgccg cagaccgcgc ccgtgccggt gcgctcgacc ggcgcctcgc
     7681 aggtggtccg gggcctcgcg cccgtggtcg gcgcgctggc gcaaagcggc ctggtggtcg
     7741 acgtgaccgt cgagggcatg ctgcacgcgc ccgagctgcc cgacatcctc gccggcggcg
     7801 cgcgcctgtt gatggtgagt aacgagcacc ccgacgtgct cgagcggctc gcgcccgcac
     7861 ccgagctcgc gcccaaggtc aaggccggca tccgcatgct caaggaggcg cagctgatgc
     7921 gcatcgagtc ggcggccggg accgatctcg tggtgcgcct tgcgggcgcg cgcgccggtg
     7981 gcgtatgggg ctggtgcgac aagccgggac aggtcgccca ctggccgggc gggctgtgcc
     8041 tgtgcttccc cgccgccagg tcggtgtcgg gtcggatcgt actcgaccgc ggcgacgtca
     8101 acctgacctt caagcgctat ctcgaatcgc cggtgaccct gacggtcgag gacgactacg
     8161 tcaccgcgat cggcggcgac gggctcgacg ccgagctgat gcggagctac ttcgccgcct
     8221 ggggcgaacg cgacgcctac gcggtcagcc acgccggctg gggcatgaac ccggcggcgc
     8281 gctgggacgc gatgaccatg tacgaccgcc gcgacatcaa cggcacggag ctgcgcgcgt
     8341 tcgccggcaa cttcctctac tcgaccggcg ccaacgaggt cgccgggcgc ttcaccgccg
     8401 gccacttcga tttgccgatg cgcggctgca cggtgcgcct ggatgaccgc gtggtggtcg
     8461 acgccggccg cctgcagggc gagcttgcat gagcgcgctc ggcgacatgc tcacccccgc
     8521 ctaccgcctg gtcggcgacg ggcccgagac gctgctctac cttcacggcc tcggcggcga
     8581 ccggaccagc ttcgacggcc agatcgcggc gcgcccgcgc ccctatcgcc acctcgcctg
     8641 ggacatgccc ggctacggcg actcggcgcc gctcgccgag atgacgttcc ccgcgctcgc
     8701 gcgcgccgcg ctgcgcctgc tcgacgatgt cggcgcggcg cgggcgcatg tggtcggcca
     8761 ctcgctcggc ggcatgatcg cgcaggagct ggtccacgcc tgtcccgagc gcatcgcctc
     8821 gctggtgctg tacgcgacca gcgccgcgtt cggcggcgcg gggggcgact ggcagcaacg
     8881 cttcctcgac gagcgcctgg cgccgctcga gcgcggcctg ggcctccgcg acctcgcccc
     8941 cgggctggtc gccgggctgg tggccgagga cgcggacccc gccggcgtgc gcgccgcgat
     9001 tgcgaccatg ggcgccgttc ccgccgccgc ctaccgcgcg gcgctcacct gcctggtcag
     9061 cttcgaccgg cgcgcggagc tcgatgcgat ccgcgttccg accctgctca tcgccggcac
     9121 ccgcgatacc gtggcgccgg ccgagatgat ggcgcgcatg gccgcgcgca tcgccagcgc
     9181 gcgcctggta cggatcgagg gcgccgggca cctcgcccat ctcgagcgcc cggacgcgtt
     9241 caacgccgcg ctcggcgggt tcctggacga gctggtcgcg cgcgggcgaa aggacgacga
     9301 tgggcttcgc gcccaccgct aggcagcgcg agctggtcga gctggccgcg cggctgggac
     9361 gcgagaagtt cgccccgcgc gccgcccgct acgacgccga ggcgtcgttc ccgttcgaga
     9421 attacgagga tctgcgcgcg gccgggctgc tcgccctgtg cgtgcccgag gcctatggcg
     9481 gcctcggcgc cgacttcgcc acctattgcc tggtctcggc cgagctcggg cgccattgcg
     9541 gcgcgaccgc gctgacctac aacatgcacg cctgcacgat gctgtggtcg gggccgatga
     9601 ccgacgacct cgacatgacc aacgccgagc gcgcgcagca cgccgagcgc caggcgctcc
     9661 atttccggcg cgtggtcaag gacggcgcga tctacgccca gccgttctcc gagggcaacc
     9721 cctcggccgc ggggcgggcg ccgttcggca ccctcgccag gcgcagcggc ggcggctggg
     9781 tgctcaacgg caagaagatc ttcgcctcgc tcgccggcgc cgcccactat tacggcatcc
     9841 tgtgcaccga ggacgtggac ggcgcctcgc gcaaggacac catctatctc gcggtcccgg
     9901 ccgacgccga cggcttcacc gtgaccgggc cgtgggaccc gctcggcatg cgcggcaccg
     9961 tctcgcgcac gctggtgctc aaggacgtgt tcgttgccga ccaggccgag ctgatgccgc
    10021 gcggcgtcta ctatcaggcg gccaaccgtt ggccgcacat gttcctgacc ctggcgccga
    10081 cctatctcgg catcgcccag gccgcctacg acttcaccgt cagctacctg cgcggcgagg
    10141 tcgagggcac cccgccggtc aagcggcgca tgtacccgac caagcagatc gcggtcgccg
    10201 agatgcacgt caagctcgag cagacctggg cgatcttcct gcgcgtgatc tccgaggcgc
    10261 gggtcgaccc cgacaaggcc gcgcggctgc gcctgctcgc cgcccagtac acgatcatgg
    10321 agaatgccaa cgacctgtgc cggcttgcga tccgcacctg cggcggccag tcgatgctga
    10381 ggagcctgcc gctcgagcgc ctctaccgcg acagccgctg cggctcgctg atgctgccct
    10441 tgaccgccga gctgtgcctc gaccgcctcg gccgcgacgc gctctacgag ccgggcgaga
    10501 ccgacgaggc gtagcggtca gccggagtga cgcttggcga tggcgccgcc cgcgatgcgc
    10561 gggatcgccg ccttcaggtc gctccagccg taggcgagct cctgcagcgg cacgccccac
    10621 atcctttccc gtcgctcggc aatcagcctg ccgcccatcg cctggtagaa gaacctggcc
    10681 gggttgtcgg ccagcaccca gatcagcgcc gagcccagcc ccttgcccgc gagcacgccg
    10741 aaggagcgcg ccagcaacgc ccggccgatc gcttgcgcct gaaagtcggg gtgcacgtag
    10801 agcatggtga cctcgccccg ataggggaga tcgagcccct tcgcgctcgc gcaactggcg
    10861 aagccgacga tgccggcatc ggggtcctcg gcgaccagga ccagttcgct ccgccgcgcg
    10921 tatttgagcg tccgcgacca
//
LOCUS       DASWHU010000002         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2218344, whole genome shotgun sequence.
ACCESSION   DASWHU010000002 DASWHU010000000
VERSION     DASWHU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218344"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1005)
                     /locus_tag="VGB88_00055"
     CDS             complement(442..1005)
                     /locus_tag="VGB88_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HEX9645857.1"
                     /translation="MSGRRRDRRPGGTAAEALPWGDTGLWDFSLAVYARPRVANTCLM
                     LQDRFGADANLILYGCWLGVTGRGALTAEDLAQLSEAATVWHKEVVVPLRGVRQWLKS
                     PPRRVARALAEALRAQVKAAELDAERIEQTVLGEALNRSPVAHERERRRRDVAQSLDA
                     YFAFIGAALDDPPRDATAALIDEVCTR"
     gene            complement(1002..2099)
                     /locus_tag="VGB88_00060"
     CDS             complement(1002..2099)
                     /locus_tag="VGB88_00060"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX9645858.1"
                     /translation="MADAGGIRRIGVLTSGGDCAGLNPIIRAVVHHAKRAYGWDVIGV
                     RYGTDGLVRRPVDAVEIDFDDVPGYVMMMSGTFLGTTSRGDPFTSRDAAGRPADLSDA
                     IVEGYRLLGLDALIGIGGDGSLSILRRLARKGGWNLVGVPKTIDNDVGLTDTAIGFAT
                     AVHVATEALDRLHFTAASHQRVMVLEVMGRDAGHIALRAGIAGAADVILIPEIPYTME
                     AIKEKLDRRVERGRNYSLIVVAESVPTEDGERVTHSTGDGQRRYGGIGHHLAARITEM
                     TGAETRVTVLGHVQRGGIPSAVDRVLGSAFGVAAVDLIAQGRFDRMVAWQGRSVVDVA
                     IEDAIASYAQVDPRDPLVHTARGLGICLGDE"
     gene            2245..2442
                     /locus_tag="VGB88_00065"
     CDS             2245..2442
                     /locus_tag="VGB88_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX9645859.1"
                     /translation="MNKEALVEQLERLRAEHEVLNSRIDEMTSGGVYDDIRILRLKKR
                     KLAIRDQIGRIESDLVPDIIA"
     gene            2593..3099
                     /gene="purE"
                     /locus_tag="VGB88_00070"
     CDS             2593..3099
                     /gene="purE"
                     /locus_tag="VGB88_00070"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9645860.1"
                     /translation="MTGPAPQVGIIMGSQSDWESMRYAAETLERLGVVFETRIVSAHR
                     TPERLYQYAAEARERGLKVIIAGAGGAAHLPGMTAAITPLPVIGVPVHSHALSGLDSL
                     LSIVQMPAGVPVATVAIGRAGAVNAALLAAAILALEDARIAAALDAWRRAQTEAVGEQ
                     PSADAGPD"
     gene            3114..>3641
                     /locus_tag="VGB88_00075"
     CDS             3114..>3641
                     /locus_tag="VGB88_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9645861.1"
                     /translation="MAAPRVIAPGAAIGILGGGQLGRMTALAAARLGYRCHVFGPEAG
                     APASQVAARTTVAGYGDAAALERFAAAVDVVTFEFENVPHEAAEALGRQVPVRPGANA
                     LAIAQDRLEEKRFFASIGAAAAAYRPVRGPDEVAAALAALGAPCVLKTTRLGYDGKGQ
                     VRIDHARDAAAAWAQL"
BASE COUNT          490 a         1297 c         1319 g          535 t
ORIGIN      
        1 ctgttcgcca acgtggtcgc ggtcgagcac atcaaggcgg cctagagcgc gatgatatta
       61 agtcggatcc ctgagcctgt tttggtcgtc accccggact ccgcgaagcg gatatccggg
      121 gtccatacgc ctttgccacg cactcgacca cccgtgcaag cggcggcatg gaccccggct
      181 cgcggcgctg cgcgccttgg ccggggtgac gggtgacgga tgaccatgtt tcaaatcctc
      241 aggctctagc gccagctatc gatgacgcac gagccgcgaa cctcatgctt cgagacgccc
      301 gcttcgcggg ctcctcatgc ttcgagacgc ccgcttcgcg ggctccgtag catgaggttt
      361 gtgagagagc cgcatcctga ggaaggcggg aggcccgtct cgaaggatgc ggcgggcagg
      421 cgcaacttgt gagtccgcat gctaacgcgt gcagacctcg tcgatcaggg ccgcggtcgc
      481 gtcgcgcggg gggtcgtcga gcgcggcgcc gatgaatgcg aaataggcgt cgaggctctg
      541 cgcgacatcg cgccgccggc gctcgcgctc gtgggcgacc ggcgagcggt tgagcgcttc
      601 gccgagcacc gtctgctcga tgcgctcggc gtcgagctcg gccgccttca cctgggcacg
      661 cagggcctcg gcgagcgccc gggcgacccg ccgcggcggc gacttgagcc actggcggac
      721 gccgcgcagc ggcaccacca cctccttgtg ccacaccgtc gccgcctcgc tgagctgcgc
      781 caggtcctcc gcggtcagcg cgccgcggcc ggtgacgccg agccagcagc cgtagaggat
      841 caggttggcg tcggcgccaa accggtcctg gagcatcagg caggtgttgg cgacccgcgg
      901 gcgcgcgtag accgcgagcg agaagtccca cagcccggtg tcgccccacg gcagcgcctc
      961 ggccgcagtc ccgcccggcc ggcgatcgcg cctgcgcccg ctcattcgtc gcccagacag
     1021 atgccgaggc cgcgcgcggt gtgcaccagc gggtcccggg gatcgacctg ggcgtagctc
     1081 gcaatcgcgt cctcgatcgc cacgtcgacc acgctccgtc cctgccaggc gaccatgcgg
     1141 tcgaaccggc cctgtgcgat caggtccacc gccgccaccc cgaacgcgga gcccagcacg
     1201 cggtcgaccg cgctcgggat gccgccgcgc tggacgtggc cgagcacggt aacgcgggtc
     1261 tcggcgcctg tcatctcggt gatgcgcgcg gccaggtgat ggccgatgcc gccgtagcgc
     1321 cgctggccgt cgccggtgct gtgggtgacc cgctcgccgt cctcggtggg caccgactcg
     1381 gcgaccacga tcaggctgta gttgcggccg cgctcgaccc gccggtcgag cttctccttg
     1441 atcgcctcca tggtgtaggg gatctcgggg atcaggatca cgtcggcggc gccggcgatg
     1501 cccgccctca gcgcgatatg gccggcgtcg cgccccatca cctcgagcac catcacccgc
     1561 tggtggctgg cggcggtgaa gtgcaggcgg tcgagcgcct cggtggcgac gtggaccgcg
     1621 gtggcgaagc cgatcgcggt gtcggtgagg ccgacgtcgt tgtcgatggt cttgggcacg
     1681 ccgaccaggt tccagccgcc cttgcgcgcc agccggcgca ggatcgacag cgagccgtcg
     1741 ccgccgatgc cgatcagcgc atcgaggccg agcagccggt agccctcgac gatggcatcc
     1801 gacaggtcgg ccgggcgccc ggccgcgtcc cggctggtga acggatcgcc ccggctggtg
     1861 gtgccgagga aggtgccgct catcatcatc acgtagccgg ggacgtcgtc gaagtcgatc
     1921 tcgaccgcat ccaccgggcg cctcaccagc ccgtcggtgc cgtagcgcac gccgatcacg
     1981 tcccagccgt aggcgcgctt ggcgtggtgc accaccgcgc ggatgatcgg gttgagcccg
     2041 gcgcagtcgc cgccgctggt cagcaccccg attcgcctga ttccccctgc gtccgccatc
     2101 cgcctcgcgc cacctcgccg gttgggtcgc cgcgtgcggc ccgcttggcg ggcgcgcagc
     2161 gaccgtcttt gccgccacgg cggcagttgc tatacttgct gctgtcattg tgtgacagat
     2221 gcaaccctgc gttcaaagga gtcgatgaac aaggaggcgc tggtcgaaca gttggagcgc
     2281 ctgcgtgccg agcatgaggt gctcaactct cgaatcgacg agatgacgtc gggcggtgtc
     2341 tacgacgaca tccgaatcct gcggctgaag aagcgcaagc tggcgatacg ggaccagata
     2401 ggccggatcg aaagcgatct ggtcccggac atcatcgcct gaccgaaggc atggtccggt
     2461 cccgggcggc ggcttgcgac cgtcggtacg aatcgcatcc tgagcctggc ccgggcccgg
     2521 ccgcgcgggc ggccgggttt cgtgttcctg gggcgtcgtg gtgggtagcg gagcggacct
     2581 ggagctttcg acatgaccgg accggcgcct caggtcggca tcatcatggg ctcgcagtcg
     2641 gactgggagt cgatgcgcta cgccgccgag acgctggagc ggctgggggt ggtgttcgag
     2701 acccgcatcg tctcggccca ccgcacgccg gagcggctgt accagtacgc ggccgaggcg
     2761 cgcgagcgcg ggctgaaggt gatcatcgcc ggcgccggcg gcgccgccca cctgcccggc
     2821 atgaccgccg ccatcacgcc gctgccggtg atcggcgtgc cggtccacag ccacgccttg
     2881 agcggcctcg acagcctgct ttcgatcgtc cagatgccgg ccggggtgcc ggtggcgacg
     2941 gtggcgatcg ggcgcgccgg cgcggtcaac gccgcgctcc ttgccgccgc gatcctggcg
     3001 cttgaggacg cgcgaatcgc cgctgcgctc gacgcctggc gccgggcgca gaccgaggcg
     3061 gtgggcgagc agccgtcggc ggacgccggt cccgactgag cgcgcgcagg ccgatggccg
     3121 cccctcgggt gatcgcgccg ggcgcggcca ttggcatcct cggcggcggc cagctcgggc
     3181 gcatgaccgc gctcgccgcg gcccggctcg gctatcgctg ccacgtgttc ggtcccgaag
     3241 ccggcgcccc ggcgagccag gtcgcggccc ggaccacggt cgcgggctat ggcgacgccg
     3301 ccgcgctgga gcggttcgcg gccgcggtcg acgtagtcac cttcgagttc gagaacgtgc
     3361 cccacgaggc ggccgaggcg ctcggtcggc aggtgccggt gcgccccggc gccaacgcgc
     3421 ttgcgatcgc ccaggaccgg ctcgaggaga agcgattctt cgcctcgatc ggcgccgcgg
     3481 cggccgccta ccggccggtg cgcggaccgg acgaggtcgc ggcggcgctc gccgcgctcg
     3541 gtgctccctg cgtgctcaag accacccgcc tcggctatga cggcaagggc caggtgcgca
     3601 tcgaccacgc gcgcgatgcg gcggcggcgt gggcgcagct c
//
LOCUS       DASWHU010000003         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1109898, whole genome shotgun sequence.
ACCESSION   DASWHU010000003 DASWHU010000000
VERSION     DASWHU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109898"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VGB88_00080"
     CDS             complement(<1..1202)
                     /locus_tag="VGB88_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9645862.1"
                     /translation="MDELARLTACEAVARLAAREVSPLELIDAAAARIAATDGAINAL
                     PTLCVERARAHAHRLMKAPPADPPPHFLHGLPIAVKDLVEVAGVRTTFGSPIFADHVS
                     AFSDYMVETLEANGAIVIGKSSTPEFGAGSHTFNEVFGHTNNPWNARLSAGGSSGGSA
                     AALAAGQVWLATGSDLGGSLRNPASFCGVVGLRPSPGRVAHGPAALPFDDLGVQGPMA
                     RTVADVALMLDAMCGRHADDPLSLDRPAVPFRAAATNAAPPARVAFSADLGGVVPVEP
                     EVAALCARAAHAFADLGAVVEQACPDFSGAADAFHVLRAHAFAVRRGVLLDQHRDKLK
                     PEIVWNIEAGRALTAADIERAECARGALFHRMSRFFERFDVLLCPAAIVPPFDGTLRY
                     PQQVAGTRL"
     gene            complement(1204..2394)
                     /locus_tag="VGB88_00085"
     CDS             complement(1204..2394)
                     /locus_tag="VGB88_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9645863.1"
                     /translation="MTQTNAMPPARGRQFLHTPGPTMMPDRILRAMDRPALDHRGPEF
                     RAIAEHCHEGLKRVFRTEAPVLILPAAGHGAWEAALANTCAPGERVLMAETGAFSAMW
                     KGMAEALGLVVEELSGDWRHGVDPAAVEAHLAADRDRRIKAVAVVHNETSTGVTSRLA
                     EVRRAIDASGHPALLIADTISSLASIDFRMDEWGVDVAIGGSQKGLMLPPGMSFNAVG
                     DKALRAAQAVTTARSYWDWRLMLPDGTTPTFTCTPAINLMFGLAEALDMLEEEGLEAC
                     LARHARLAEAVRRAVAGWGLELWSKDAREHSNSISAVLMPDGHDADALRSLCRERFNL
                     VLAGGLMRLKGRLFRIGHLGDLNEPMILGTLATVEMGLRAAGVPHRPGGVDAAIAYLA
                     EHGA"
     gene            2430..>5286
                     /locus_tag="VGB88_00090"
     CDS             2430..>5286
                     /locus_tag="VGB88_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9645864.1"
                     /translation="MASLVADKSRIRDSALAARLRRAVAGEVLFDPFSRGRYATDASI
                     YQIEPLGVVVAKSAGDIEAAVAIAREAGVAVLPRGGGTSQCGQTVGEALVVDTSKYLR
                     GVRDLDLDRRRVTVEPGLVLDQLNAWLKPHGLWFPVDVSTGSRATIGGMAGNNSCGAR
                     SIRYGNMVHNVHAIDAVLAGGVRHRFGPVPGDLDGLDADAGYAALVRDMRALGRREAD
                     EIKARFPKLLRRVGGYNIDTVSSAGHNMAQLLVGSEGTLGFFTAIELALSPLPAHSAL
                     GVCHFTSLRAAMAAARDIVALEPAAVELADRSLIELAREVPLYRATIERFMAGDPEAV
                     LMVEFAGDDGPAQAAGLDRLDELLADLGHAGTVIKVTDRAEQKAVWEVRKAGLNILMS
                     MKGDGKPVSFVEDCAVDLADLADYTDRMSAIFARHGTRGTWYAHASVGCLHVRPVLNM
                     KDAGDVAKMRAIAEEAFDMVRAYGGSHSGEHGDGLVRSEFHERMFGPRLVRAFEQVKD
                     RFDPDGVFNPGKIVRPSRMDDRALFRYRPGYAPARFAPALDWSAWGAGDGTGGFLAAV
                     EMCNNNGACRKRDASVMCPSYRATGDEQHLTRGRANTLRLALTGQLGADALESDEMAA
                     TMRLCVGCKGCKRECPTGVDMARMKIEVLHRRARRRGVGLRDRLIGYLPRYAPAAARL
                     RAVVKMRNRSRALRWAGERALGLSARRALPEWPRDPFLETPAATGDGPEVVLFADTFT
                     TWHEPGNARAALRVLGAAGYRVHVARAAAGDGRRPLCCGRTFLAVGLVDEARAEARRS
                     LRALAPYVARALPVVGLEPSCLFTFGDEMPALLPGDDTAQALARQAVMLESFLVAEHA
                     AGRLDLALGALDRDTALVHGHCHQKAFGVASDIASVLGWVPGLAVEPIESTCCGMAGA
                     FGYQAETAEVSLSIGELDLFPALRRPERAGALVVAGGT"
BASE COUNT          694 a         1904 c         1980 g          708 t
ORIGIN      
        1 aggcgcgtgc cggcgacctg ttgcgggtag cgcaacgtgc cgtcgaacgg cggcacgatc
       61 gccgccgggc acagcagcac gtcgaagcgc tcgaagaacc ggctcatgcg atggaacagc
      121 gccccgcgcg cgcactcggc gcgctcgatg tcggccgcgg tgagcgcgcg cccggcctcg
      181 atgttccaca cgatctcggg cttgagcttg tcgcggtgct ggtccagcag cacgccgcgc
      241 ctgaccgcga acgcgtgcgc gcgcagcacg tggaaggcgt cggccgcgcc gctgaagtcc
      301 gggcacgcct gctcgacaac ggcgccgaga tcggcgaagg cgtgcgcggc gcgtgcgcac
      361 agcgccgcga cctcaggctc gaccggcacg acgccgccga ggtcggcgct gaaggcgacg
      421 cgcgccggcg gcgccgcgtt cgtcgccgcg gcgcgaaacg gcaccgccgg gcggtcgagc
      481 gacagcggat cgtccgcatg ccggccgcac atggcgtcga gcatcagcgc cacgtcggcg
      541 acggtgcgcg ccatcggccc ctgcacgccg aggtcgtcga agggcagtgc ggcgggcccg
      601 tgcgcgaccc ggcccgggct cgggcgcagg ccgaccacgc cacagaagct cgccgggttg
      661 cgcagcgagc cgccgaggtc ggaaccggtg gcgagccata cctggcccgc cgccagcgcc
      721 gccgccgagc cgcccgacga gccgccggcg ctgaggcgcg cgttccacgg gttgttggtg
      781 tggccgaaca cctcgttgaa ggtgtgcgag ccggcgccga actcgggcgt gctcgacttg
      841 ccgatcacga tcgcgccgtt ggcctccagc gtctcgacca tgtagtccga gaaggcggag
      901 acgtggtcgg cgaagatcgg cgagccgaag gtggtgcgca cgccggcgac ctcgaccagg
      961 tccttgaccg cgatcggcag gccgtggagg aagtggggcg gcggatcggc cggcggcgcc
     1021 ttcatcaggc gatgcgcgtg tgcccgcgcg cgctcgacgc agagcgtcgg cagcgcgttg
     1081 atcgcgccgt cggtggccgc gatgcgcgcc gcggcggcgt cgatcagctc gagcggcgag
     1141 acctcgcgcg ccgcaagccg cgccaccgcc tcgcacgcgg tcagccgggc gagctcgtcc
     1201 atgtcacgcg ccgtgctcgg cgaggtaggc gatcgcggcg tcgacgccgc ccgggcgatg
     1261 cggcacgccg gctgcgcgca ggcccatctc gaccgtcgcc agggtgccga ggatcatcgg
     1321 ctcgttgagg tcgccgagat ggccgatgcg gaacaggcgg cccttgagcc gcatcaggcc
     1381 gccggcgagc accaggttga agcgctcgcg gcacaggctg cgcaatgcat cggcgtcgtg
     1441 gccgtcgggc atcagcaccg ccgagatcga gttggagtgc tcgcgcgcgt ccttcgacca
     1501 cagctcgagg ccccagcccg cgaccgcgcg tcgcaccgcc tcggcgagcc gcgcgtggcg
     1561 cgcgaggcac gcctcgagcc cttcctcctc gagcatgtcg agagcttcgg ccagcccgaa
     1621 catcaggttg atcgccggcg tgcaggtgaa ggtcggcgtc gtgccgtcgg gcagcatcag
     1681 gcgccagtcc cagtagctgc gcgctgtcgt taccgcttgc gccgcgcgca gcgccttgtc
     1741 gccgacggcg ttgaacgaca tgccgggcgg cagcatcagc cccttctgcg agccgccgat
     1801 ggcgacgtcg acgccccact cgtccatgcg gaagtcgatc gaggcgagcg aggagatggt
     1861 gtcggcgatc aggagcgccg gatgcccgga ggcgtcgatc gcccggcgca cctcggcgag
     1921 gcgcgaggtg accccggtcg aggtctcgtt gtggaccacg gcgaccgcct tgatgcgccg
     1981 gtcgcggtcg gcggcgagat gcgcctcgac ggcggccggg tcgacgccgt ggcgccagtc
     2041 gccggaaagc tcctcgacca ccaggccgag cgcctcggcc atgcccttcc acatcgccga
     2101 gaacgcgccg gtctcggcca tcagcacccg ctcgcccggg gcgcaagtgt tggcgagcgc
     2161 ggcctcccac gcgccgtggc cggcggcggg gaggatcagc accggcgcct cggtgcggaa
     2221 gacgcgcttg aggccctcgt ggcagtgctc ggcgatggcg cggaactcgg gcccgcggtg
     2281 gtcgagcgcc ggccggtcca tcgcccgcag gattcggtcg ggcatcatgg tcgggccggg
     2341 ggtgtgcaga aattgtcgtc cgcgcgcagg cggcatcgcg ttagtctggg tcatggctcc
     2401 acgcaattcg agtcggccgg cgcggcaagg tggcgagtct ggtagcagat aaatcgcgca
     2461 tccgcgacag cgctttggcc gccaggctca ggcgcgcagt cgcgggcgag gtgctgttcg
     2521 atccgttctc gcgcgggcgc tacgccaccg atgcgtcgat ctaccagatc gagccgctgg
     2581 gcgtggtggt ggcgaaaagc gcgggcgaca tcgaggccgc ggtggcgatc gcgcgcgagg
     2641 cgggggtggc ggtgctgccg cgcggcggcg gcacctcgca atgcggccag acggtgggcg
     2701 aggcgctggt cgtcgacacc tcgaaatatc tgcgcggggt gcgcgacctc gacctcgacc
     2761 ggcgccgggt gacggtcgag ccggggctgg tgctcgacca gctcaacgcc tggctcaagc
     2821 cgcacgggct gtggttcccg gtcgacgtct cgaccggcag ccgcgcgacc atcggcggca
     2881 tggccggcaa caacagctgc ggcgcgcgct cgatccgcta cggcaacatg gtccataacg
     2941 tgcacgcgat cgacgccgtg ctggcgggcg gcgtgcgcca ccgcttcggc ccggtgcccg
     3001 gcgacctcga cgggctggac gcggacgcgg gctatgccgc cctggtccgg gacatgcgtg
     3061 cgctcggccg gcgcgaggcg gacgagatca aggcgcgctt cccgaagctc ctgcgccggg
     3121 tcggcggcta caacatcgac accgtctcgt ccgccggcca caacatggcg cagctcctgg
     3181 tcggctccga gggcacgctc ggcttcttca ccgcgatcga gctcgcgctt tcgccgctgc
     3241 cggcgcacag cgcgctcggc gtctgccatt tcacgtcgct gcgcgcggcg atggcggcgg
     3301 cgcgcgacat cgtcgcgctc gagccggcgg cggtcgagct cgccgaccgc agcctgatcg
     3361 agcttgcgcg cgaggtgccg ctctaccgcg cgaccatcga gcggttcatg gcgggcgatc
     3421 cggaagcggt gctgatggtc gagttcgccg gcgacgacgg gcccgcgcag gccgccgggc
     3481 tcgaccggct cgacgagctg ctcgccgacc tcggccacgc cggcacggtc atcaaggtga
     3541 ccgaccgcgc cgagcagaag gcggtgtggg aggtgcgcaa ggcgggcctc aacatcctga
     3601 tgtcgatgaa gggcgacggc aagccggtgt cgttcgtcga ggactgcgcg gtcgacctcg
     3661 cggacctcgc cgactacacc gaccggatga gcgcgatctt cgcccggcac ggcacccgcg
     3721 gcacctggta cgcgcacgcc tcggtcggct gcctgcacgt gcgcccggtg ctcaacatga
     3781 aggacgcggg cgacgtggcg aagatgcgcg ccatcgccga ggaggcgttc gacatggtgc
     3841 gcgcctatgg cggctcgcat tcgggcgagc acggcgacgg cctggtgcgc tccgagttcc
     3901 acgagcgcat gttcggcccg cgcctggtgc gcgcgttcga acaggtcaag gaccgcttcg
     3961 accccgacgg cgtgttcaat ccgggcaaga tcgtgcggcc gtcgcgcatg gacgaccgcg
     4021 ccctgttccg ctaccggccg ggctacgcgc cggcgcggtt cgcgcccgcg ctcgactggt
     4081 cggcgtgggg cgcgggcgac ggcaccggcg ggttcctggc cgcggtcgag atgtgcaaca
     4141 acaacggcgc ctgccgcaag cgcgacgcct ccgtcatgtg cccgtcctac cgcgccaccg
     4201 gcgacgagca gcacctgacc cgcgggcgcg ccaacacgct tcgcctcgcg ctgaccggcc
     4261 agctcggcgc ggatgcgctc gagtcggacg agatggcggc gaccatgcgc ctgtgcgtcg
     4321 gctgcaaggg ctgcaagcgc gagtgcccga ccggcgtcga catggcgcgc atgaagatcg
     4381 aggtgctgca ccgccgcgcc cgccggcgcg gggtcggctt gcgcgaccgg ctgatcggat
     4441 acttgccgcg ctatgcaccg gcggcggcgc gcttgcgcgc ggtggtcaag atgcgcaacc
     4501 gctcgcgcgc gctgcgctgg gcgggcgagc gcgcgctcgg attgagcgcc cgccgcgcct
     4561 tgcccgagtg gccccgcgac ccgttcctgg agacgcccgc cgcgaccggc gacggccccg
     4621 aagtcgtgct gttcgccgac accttcacca cctggcacga gcccggcaac gcccgcgccg
     4681 cgctgagggt gctgggcgcg gccggctatc gcgtgcacgt cgcgcgcgcg gccgccggcg
     4741 acggccgccg gccgctgtgc tgcgggcgca ccttcctcgc ggtcggcctg gtcgatgagg
     4801 cgcgcgcgga agcccggcgg agcttgcgcg cgctcgctcc ttacgtcgcg cgcgcactcc
     4861 cggtggtcgg cctcgagccg tcctgcctgt tcaccttcgg cgacgagatg ccggcgctgc
     4921 tgcccggcga cgacaccgca caggcgctcg cgcgtcaggc ggtgatgctc gagtcgttcc
     4981 tggtcgcgga gcacgccgcc gggcgtctcg acctcgcgct cggtgcgctc gaccgcgaca
     5041 ctgcgctggt ccacggccat tgccaccaga aggcgttcgg agtcgccagc gacatcgcca
     5101 gcgtgctcgg ctgggtgccg gggctcgccg tcgagccgat cgagtcgacc tgctgcggca
     5161 tggccggcgc gttcggctat caggccgaga ccgccgaggt ttcgctcagc atcggcgagc
     5221 tcgacctgtt tcccgcgctg cgccgccctg agcgcgccgg cgccctggtc gtcgccggcg
     5281 gcacca
//
LOCUS       DASWHU010000004         7001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3327199, whole genome shotgun sequence.
ACCESSION   DASWHU010000004 DASWHU010000000
VERSION     DASWHU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3327199"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VGB88_00095"
     CDS             <1..1095
                     /locus_tag="VGB88_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9645865.1"
                     /translation="DQVAAGAAHGLNPLVANYGPALLDRAVLDALCRVRGISVFNAMA
                     SNLAGITNCSLVPDLDGFDMDAFVRSLRPARAIAARHTVGLVDAIGDPSGGPDDGLPR
                     TLREAIAAYGHRWFKLKVAGDLDADLARLAEIAAELDRIDGAYHVTLDGNEQYRDADG
                     VALLLERIAATPALGRLAGAIAFIEQPIARGAALDTDVTAIAGRWPLLIDESDATLAA
                     FTRARALGYAGVSSKSCKGLYKSILNVARCRMWNEHAGAPRAFMSAEDLSTQAGLAVQ
                     QDLALATLIGCRHVERNGHHYVNGMAGVPETEQAAFLAAHADLYHRAHGVVRLTITGG
                     DIALGSLACPGFAAGAEPDWDALRPMPRAA"
     gene            1097..1921
                     /locus_tag="VGB88_00100"
     CDS             1097..1921
                     /locus_tag="VGB88_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX9645866.1"
                     /translation="MAELARLSLNLATTRIKWGAREAIEGCARAGVRWIAPWRDQIAK
                     DGLEACVRAVRDAGLEVSSVCRGGMFPAGDAAARQAAIEDNKRAIDEAAALGARALVL
                     VVGGLPAGSKDLAGARAMVAEGIAAVLPYAREAGVALAIEPLHPMYAADRACINTLAH
                     ALDACDALGAGVGVAVDVYHVWWDPDLGPAIARAGRDRLLGFHVSDWLVPTRDLLLDR
                     GMMGDGVIDIPRITALMDRAGYDGPIEVEIFSAGNWWQRDPDEVLSIAKQRFASAV"
     gene            2097..2342
                     /locus_tag="VGB88_00105"
     CDS             2097..2342
                     /locus_tag="VGB88_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645867.1"
                     /translation="MCLAFGWAHVWLHDAGHDKGLHFRSEATAQCQLAKLHWQILPEP
                     PSCAARLGFDAPSADEPRIAFARAATRRPGVRAPPLS"
     gene            2472..3158
                     /locus_tag="VGB88_00110"
     CDS             2472..3158
                     /locus_tag="VGB88_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9645868.1"
                     /translation="MNDQDRAGTRSMLALDGVRHRYDRTEVVAVEHWQVAEGEQWLLH
                     GPSGCGKSTLLHIMAGLLTPSQGTVRLAGQDLGALAGAARDRFRGRHVGIVFQQLHLI
                     DALTVRQNLVLAQSLAGLPPNEARVGEMLDGLRIADKADARPFALSYGQAQRVALARA
                     LINRPQLLLADEPTSNLDDANAERALALLRDQAEAQGSALVVASHDGRIKDTFERRLE
                     LDIAAEAQAA"
     gene            3155..4426
                     /locus_tag="VGB88_00115"
     CDS             3155..4426
                     /locus_tag="VGB88_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9645869.1"
                     /translation="MNALALSLAYLRRRPLNTVLNLLLLAIGVGTISLLLLFSDQLQE
                     RLARDAQGVDMVVGAKGSPMQLILSSVFHIDVPTGNVRIADARPLTENRMIEQVIPLA
                     LGDSFRGFRIVGTERAYADNYSAELAEGAWWQASMEATVGSVAAAQAGLGVGTSFVGS
                     HGLAEGGAMHGEHPYRVVGVMAPTGTVLDRVILVSVESVWDVHEIHHGGEAEGEDHDA
                     HESAAGSAGDGSRESASVLETGDDDLELTALLVRFRNPIGAATMPRQINMTTPMQAAL
                     PGYEIARLMQMIGIGLDGFRAFAAVLVVSAGLGIFTALYNALAQRRYDIAVMRSLGAS
                     RGAILRQILLEGTLLAFAGGLLGLILGHAVAEVAGAWLWREHQIYLRGLAWDADEGWL
                     AVMALLLGAVAALLPALQAYRIDISRVLARG"
     gene            complement(4440..4931)
                     /locus_tag="VGB88_00120"
     CDS             complement(4440..4931)
                     /locus_tag="VGB88_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645870.1"
                     /translation="MPKRIAIWLGAAAFAVAGALAPAQAQTKYDRPPGDTLYPLPEVS
                     GAVPWELLVNVDMVYEGVDLVPEYSDEVRALVGETVKMVGFLLPLDAARTHVLLSMIS
                     PNCPYCLPGGPETFVELIADEPIEYSNDAVVVTGRFELLHDSLAGYYYRMTEVARAAA
                     DSS"
     gene            complement(4961..5248)
                     /locus_tag="VGB88_00125"
     CDS             complement(4961..5248)
                     /locus_tag="VGB88_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645871.1"
                     /translation="MAALAVVLMASTPVHALEIANDHDEAAKILIEGWVQFMMPHRSA
                     KFRPFEDPTLIKIELNVVRIQCEAGPDDQVRLADNNCYVNGELAGEGQFRM"
     gene            complement(5360..6385)
                     /locus_tag="VGB88_00130"
     CDS             complement(5360..6385)
                     /locus_tag="VGB88_00130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9645872.1"
                     /translation="MSIAGVASAQAPVDDKALTEKWWPSEYGPDDMVGSPNRTTPEMV
                     MKAVGLVKQGKTATLGKLYQSDVPLFGPRTYQMSIPGTPTGGPFGKNALVYHDEMLTT
                     EIGQVSTQFDGPGHIGVNTSKGMFWYNGRKHPDFYQRGAGGRVVGLGPLGVEAVAEKG
                     FICRGVLLDAPKFKGMNPLPIPTTTDSPGIVTADDVKAMVKAEGLAEIGEGDCVFLYT
                     GHGDVWANKKWPTMSPEQRAEARKYFTSGEPGFGVSACQYMASRKIILTGGDTSANDA
                     QPVGEQEGYAVPCHTEMQTRRGIWNIENLDFGPLLEAGVYEFAFVWAPLKLVGATGSP
                     GNPVAMW"
     gene            complement(6608..>7001)
                     /locus_tag="VGB88_00135"
     CDS             complement(6608..>7001)
                     /locus_tag="VGB88_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9645873.1"
                     /translation="HLCFGNYGGQVIQRGSWDKLIAYLNSLQVDHIVMEAAHRPAGEL
                     AAFRDLRPGIGLGLGVVDVKRTEVESADEIARAIERAEAVLGPGRVRYIHPDCGFWML
                     DRSIADAKIRALVAGRDLHHGGAGSRAA"
BASE COUNT         1004 a         2520 c         2396 g         1081 t
ORIGIN      
        1 gaccaggtcg ccgccggcgc ggcgcacggc ctcaacccgc tggtcgccaa ttacggcccc
       61 gccctgctcg accgcgccgt gctcgacgcg ctgtgccgcg ttcggggcat ctcggtcttc
      121 aatgccatgg caagcaacct cgccggcatc acgaactgtt cgctggtgcc cgacctcgac
      181 ggcttcgaca tggatgcgtt cgtgcgcagc ctgcgcccgg cgcgagcgat cgccgcccgc
      241 cataccgtcg gcctggtcga tgcgatcggg gatccgagcg gcgggcccga cgacggcctg
      301 ccgcggacct tgcgcgaggc gatcgccgcc tacggccacc gctggttcaa gctcaaggtc
      361 gcgggcgacc tcgacgccga cctcgcgcgc ctcgccgaga ttgccgccga gctcgaccgc
      421 atcgacggcg cgtatcatgt caccctcgac ggcaacgagc agtaccgcga cgcggacggc
      481 gtcgccctcc tgctcgagcg catcgccgcc acccccgcgc ttggccgcct ggccggcgcg
      541 atcgcgttca tcgagcagcc gatcgcgcgc ggcgccgcgc tcgacaccga tgtcaccgcc
      601 atcgccggcc gctggccgct gctgatcgac gagtcggacg cgaccctcgc tgcgttcacg
      661 cgcgcgcgcg cgctcggcta tgcgggcgtc tcgtcgaaga gctgcaaggg cctatacaag
      721 tcgatcctca atgtcgcccg ctgccggatg tggaacgagc acgccggcgc gccgcgcgcg
      781 ttcatgtcgg ccgaagacct gtcgacccag gccgggctcg cggtccagca ggacctcgcg
      841 ctcgccaccc tgatcggctg ccgccacgtc gagcgcaacg gccaccacta cgtcaacggc
      901 atggccggcg tgcccgagac cgagcaggct gcgttcctcg ccgcgcatgc ggacctctat
      961 caccgcgcac acggcgtcgt gcggctgacg atcacgggcg gcgacatcgc gctcggctcg
     1021 ctcgcctgcc ccggcttcgc cgccggcgcc gagccggact gggacgcgct gcggccgatg
     1081 ccgcgggcgg cgtgacgtgg ccgaactcgc gcgcctgtcg ctcaacctcg ccaccacgcg
     1141 catcaagtgg ggcgcacgcg aagcgatcga gggttgcgcg cgcgccggcg tccgctggat
     1201 tgcgccgtgg cgcgaccaga ttgcgaagga cgggctcgag gcctgcgtgc gcgcggtgcg
     1261 cgacgccggg ctcgaggtca gcagcgtctg tcgcggcggc atgttcccgg ccggcgacgc
     1321 cgccgcacgc caagccgcga tcgaggacaa caagcgcgcc atcgacgagg ccgcggcgct
     1381 cggcgcgcgc gccctcgtgc tggtggtcgg cggcctgccc gcgggctcga aggacctcgc
     1441 gggcgcgcgc gcgatggtcg ccgagggcat cgccgcggtg ctgccatacg cgcgcgaagc
     1501 gggcgtcgcg ctcgccatcg agccgctgca cccgatgtat gcggccgacc gcgcctgcat
     1561 caacaccctc gcgcacgcgc tcgatgcgtg cgacgcgctg ggcgccgggg tcggagtcgc
     1621 ggtcgacgtc taccacgtct ggtgggaccc ggaccttggc cccgccatcg cccgcgccgg
     1681 gcgcgaccgc ctgctcggct tccacgtctc ggactggctg gtgccgaccc gcgacctgtt
     1741 gctcgaccgc ggcatgatgg gcgacggcgt gatcgacatc ccgcgcatca ccgcgctgat
     1801 ggaccgcgcc ggctacgacg gcccgatcga ggtcgagatc ttctcggccg ggaattggtg
     1861 gcagcgcgat ccggacgagg tcttaagcat cgccaagcag cgcttcgcaa gcgcggtctg
     1921 agggttggcg ccggccggcc cggctaggcg tggtgacaat gccgccgcgg ggggttaacc
     1981 tgcaaagcgg ttgccgagga gggagacgtg gtctcggcca tcgtcacgcg gccatgacgg
     2041 agagcctcgc gacccggcgc acgcgacgtc ggcgcctgat cgcttggctc gcggccgtgt
     2101 gtctggcgtt cggctgggcg catgtgtggc tccacgacgc cggccacgac aaggggctgc
     2161 atttccgctc cgaggccacc gcgcagtgcc agctcgccaa gctgcactgg cagatcctgc
     2221 ccgagcctcc gtcctgcgcc gcacggttgg gcttcgatgc gccgtccgcg gacgagccca
     2281 gaatcgcgtt cgcccgcgct gcgacccggc gtcccggcgt gcgcgcgccg ccgttgtcct
     2341 gacgcttcag cgccgatcgc cgtccaggcc ccgccggccc gcgcgttgcg gggaccgcgc
     2401 cgcctggcgg tgagacggtc atgctcaccg gccatccggg tgagagagga cgaccaccga
     2461 ggagatcgca gatgaatgac caggaccgcg ccggcacgcg cagcatgctc gcgctcgacg
     2521 gcgtccgcca ccgctacgac cgcaccgagg tggtggcggt cgaacattgg caggtggcgg
     2581 agggcgagca gtggctgctg cacggcccgt cgggatgcgg caagagcacg ctgttgcaca
     2641 tcatggccgg cctgctgacg ccgagccaag gcacggtgcg gctcgccggc caggacctcg
     2701 gcgcactcgc cggcgccgcc cgcgaccgct tccgcggccg ccatgtcggc atcgtgttcc
     2761 agcaactgca cctgatcgat gcgcttacag tgcgccagaa cctggtgctg gctcagtcgc
     2821 tcgccggcct gccgcccaat gaggcgcgcg tcggggagat gctcgacggc ctgcgcatcg
     2881 ccgacaaggc cgatgcccgg ccgtttgccc tgagctacgg gcaagcgcag cgcgtcgccc
     2941 tcgcccgggc gctgatcaat cggccccagc tgctgctcgc cgacgagccg acctcgaacc
     3001 tcgacgacgc caatgcggag cgtgcgctgg cgctgctgcg cgatcaggcc gaggcacaag
     3061 gcagcgcgct ggtcgtcgcc agccatgacg ggcgcatcaa ggacacgttc gagcgccggc
     3121 tcgagctcga cattgcggcc gaggcgcagg cggcatgaac gcgctcgcgc tgtcgctcgc
     3181 ctatctcagg cgcaggcctc tgaataccgt gctcaacctg ctcctgctcg ccatcggcgt
     3241 cggcaccatt tcattgctgc tgctgttcag cgaccagctg caggagcggc ttgcccgcga
     3301 cgcccaagga gtcgacatgg tggtcggcgc caagggcagc ccgatgcagc tgatcctgtc
     3361 gagcgtgttc cacatcgacg tgccgaccgg caacgtgcgc atcgccgatg cccggccgct
     3421 gaccgagaac cgcatgatcg agcaggtgat cccgcttgcg cttggcgaca gcttccgcgg
     3481 cttccgcatc gtcggcaccg agcgcgccta tgccgacaac tacagtgccg agctcgccga
     3541 gggcgcatgg tggcaggcct cgatggaagc gactgtcggc tcggtcgccg ccgcccaggc
     3601 cggactcggc gtcggcacca gcttcgtcgg ctcgcacggt cttgccgaag gtggcgccat
     3661 gcacggcgag catccctatc gggtggtcgg cgtgatggcg ccgaccggca ccgtgcttga
     3721 ccgcgtcatc ctggtctcgg tggaaagcgt ctgggacgtg cacgagatcc accacggagg
     3781 cgaggcggag ggcgaggacc acgacgcgca cgagtccgcg gcgggctcgg cgggcgacgg
     3841 aagccgcgaa tcggcgtcgg tgctcgagac cggcgacgac gacctggagc tgaccgcgct
     3901 gctggtgcgt ttccgcaacc cgatcggcgc ggccaccatg ccccggcaga tcaacatgac
     3961 cacgccgatg caggcggcgc tgccgggcta cgagatcgcg cgcctgatgc agatgatcgg
     4021 catcggcctc gacggctttc gcgccttcgc cgccgtgctg gtggtcagcg ccgggctcgg
     4081 catcttcacc gcgctctaca acgcccttgc ccagcgccgc tacgacatcg cggtgatgcg
     4141 ttcgctcggc gcatcgcgcg gcgcgatcct gcgccagatc ctgctcgagg gcaccctgct
     4201 cgcctttgcc ggcggcttgc tcggcctgat cctcggccat gccgtcgccg aggtcgccgg
     4261 cgcctggctg tggcgcgagc atcagatcta cctgcgcggc cttgcctggg acgcggacga
     4321 gggttggctg gcggtaatgg cgctgttact gggtgcggtg gcggcgctgc tgccggcgct
     4381 gcaggcctat cggatcgaca tctcgcgggt gctcgcgcgc ggctgaggtg gccggcggtc
     4441 tatgacgaat cggcggcggc ccgtgcgacc tcggtcatgc ggtagtagta gcccgccaac
     4501 gagtcgtgga gcaactcgaa ccgtccggtg acgacgaccg cgtcattgct gtactcgatc
     4561 ggctcgtcgg cgatcagctc gacgaaggtc tcgggcccgc cgggtaggca gtacgggcag
     4621 ttgggcgaga tcatgctcaa caggacgtgc gtgcgtgccg cgtcgagcgg cagcaggaag
     4681 ccgaccatct tgaccgtctc gccgaccagc gcgcgcacct cgtcactgta ctcggggacc
     4741 aggtcgacgc cctcgtaaac catgtcgacg ttgaccaaca gctcccacgg caccgcgccc
     4801 gacacctcgg gcagcgggta gagcgtgtcg cccggcggcc ggtcgtactt cgtttgcgcc
     4861 tgtgccggcg caagcgcgcc cgccaccgcg aaggcggcgg cgccgagcca gattgcgatc
     4921 ctcttcggca tggtcctcat gaggctcgcg gccactcccg tcacatcctg aactggcctt
     4981 cgccggcaag ctcgccgttg acgtagcaat tgttgtcggc gagccgcacc tggtcgtcgg
     5041 ggccggcctc gcattgtatg cgcacgacgt tgagctcgat cttgatcaag gtcgggtcct
     5101 cgaacgggcg aaacttggcc gagcgatgcg gcatcatgaa ctgcacccag ccctcgatca
     5161 agatcttcgc cgcctcgtcg tgatcgttgg cgatctccag cgcgtgcacg ggcgtactcg
     5221 ccatcagaac gaccgcaagc gcggccatca gcgactgcgt cccaaccctc acttggccgt
     5281 ctcctggaaa cgaagcggga gccggcgcag atgccggctc ccgacctcaa ggtcttaaac
     5341 tactgtcgaa tggcggccct taccacatgg ccaccgggtt gccgggcgag ccggtcgcgc
     5401 cgaccagctt caacggcgcc cacacgaacg cgaactcgta gacccccgcc tcgagcaaag
     5461 gcccgaagtc gaggttctcg atgttccaga tgccgcgccg ggtctgcatc tcggtgtggc
     5521 acggcaccgc gtagccttcc tgctcgccga ccggctgggc gtcgttcgcc gaggtgtcgc
     5581 cgccggtcag gatgatcttg cgcgaggcca tgtactggca cgcgctgacc ccgaagccgg
     5641 gctcgcccga ggtgaagtac ttgcgcgctt ccgcccgctg ctccgggctc atggtcggcc
     5701 acttcttgtt cgcccacacg tcgccgtggc cggtgtagag gaacacgcag tcgccctcgc
     5761 cgatctcggc caggccctcg gccttgacca tcgccttgac gtcgtcggcg gtgacgatcc
     5821 ccgggctgtc ggtggtggtc gggatcggca gcgggttcat gcccttgaac ttgggcgcat
     5881 cgagcagcac cccgcggcag atgaagccct tctcggcgac ggcctcgacc ccgagcggcc
     5941 ccaggccgac cacgcgcccg ccggcgccgc gctgatagaa gtccgggtgc ttgcgcccgt
     6001 tgtaccagaa catgcccttc gaagtgttga cgccgatatg gccggggccg tcgaactggg
     6061 tgctgacctg gccgatctcg gtggtcagca tctcgtcgtg atagaccagg gcgttcttac
     6121 cgaacggccc gccggtcggg gtgccgggaa tcgacatctg gtaagtgcgc gggccgaaca
     6181 gcggcacgtc ggactggtac agcttgccaa gggtcgcggt cttgccctgc ttgaccagcc
     6241 cgaccgcctt catgaccatc tccggagtcg tccggttggg cgagccgacc atgtcgtccg
     6301 gcccgtactc ggacggccac catttctcag tcaacgcctt gtcgtcgacc ggcgcctggg
     6361 cgctggcgac cccggcgatc gacacgcaca aggctgcggc cgtcgcaaac ttgataaaat
     6421 cagcagtcct catcgactgg tttcctccca ttgaattcgg gggtcggcac gccccgatcc
     6481 gattcaagtc ccatggttgg gtgccgacat tccccggatg gcgcgcaccc ggagttcacc
     6541 caaccatagc accccgtccg gtggggatcg actcggaagt gccgccaggt agacgaccct
     6601 ggacagctca cgccgcgcgg ctgccggcgc cgccatgatg gaggtcgcgg cccgcgacca
     6661 gcgccctgat cttggcgtcg gcgatgctgc ggtcgagcat ccagaagccg cagtcggggt
     6721 ggatatagcg cacccgcccc ggcccgagca cggcctcggc gcgctcgatc gcgcgcgcga
     6781 tctcgtccgc gctctcgacc tcggtgcgct tgacgtcgac cacgccgagg ccgaggccga
     6841 tcccgggcct gaggtcgcgg aacgcggcga gctcgcccgc cgggcggtgc gcggcctcca
     6901 tcacgatgtg gtcgacctgg aggctgttca ggtacgcgat cagcttgtcc cacgagccgc
     6961 gctggatcac ctggccgccg tagttgccga agcacaggtg g
//
LOCUS       DASWHU010000005         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_740955, whole genome shotgun sequence.
ACCESSION   DASWHU010000005 DASWHU010000000
VERSION     DASWHU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740955"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VGB88_00140"
     CDS             <1..888
                     /locus_tag="VGB88_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX9645874.1"
                     /translation="DLPEGAAFIPEMPGTAPGLVCPLGAQVIYAVPGVPHEMRTMVGA
                     TVLADLKRRAGLRAVIRSRVLRTWGHTESGLAELLAGRIAELDALGNPTLAFQASGIE
                     GLKVRITAKAGDDAAAEAMLADEEARVRALVGDVVFGVDGETMESVVLAMLAARGLSL
                     AVAEQLSAGLVAMRLTAAANGADGTFRGGVVTAADGRASELLGAPPGPAVSAEAAAAM
                     AERVRALFGTDVGLATCGVAGPEPQDGHRPGTAFLGLALGNRAEATEARLPGDRDRIR
                     QYAVISVLNALRRRLAAAQ"
     gene            920..1474
                     /locus_tag="VGB88_00145"
     CDS             920..1474
                     /locus_tag="VGB88_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9645875.1"
                     /translation="MSRIPTVAPKDMTDAQRRVVEAIRSGPRGSAARGPFAAWLASPE
                     LADRAQHLGAYLRFETSVPARLKELAILYVARAWTAQFEWYAHKRFALDGGLGPEVID
                     AIQARRRPDFTRDDEAAVYDFCAELHERHQVSDARFQAVKKHLGQAGVVELVGVLGYY
                     TLVSMTLNVFEVAMPEGEPPPLAD"
     gene            1666..2541
                     /locus_tag="VGB88_00150"
     CDS             1666..2541
                     /locus_tag="VGB88_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9645876.1"
                     /translation="MVAPLTPRAAAVRAALWMCGTLVSFSIMAVAIRELSASQGTFQI
                     LAWRSIVGLAVLVPVALLRGRGLLATRQLRWQVRRNLVHFLGQAGWTYGIAVLPLTVV
                     FTLEFTMPAWTALLAVLFLGERLSRGRALAIAGGIAGVVVILRPDVALPDPAALIVLA
                     AAMAYAAAHTMTKQLTRSDGALAILFWMSAIQLPLGAGVALALAGGDWPLPGWVAAPW
                     IALVGLAALAAHFCLARALKLADATVVMPLDFLRLPLIAGVGAVLYGEAPTPAVALGA
                     AIIVLSLAFALRAER"
     gene            complement(2538..2825)
                     /locus_tag="VGB88_00155"
     CDS             complement(2538..2825)
                     /locus_tag="VGB88_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9645877.1"
                     /translation="MPMHDPPHPGAVIRRQCLEPLGLTVTAAAKGLGVSRNTLSMLLN
                     GRLSISPEMAIRLSKAFGGSAESWLRQQMQYDLWHAGRKAAGIKVVSFRAA"
     gene            complement(2835..3113)
                     /locus_tag="VGB88_00160"
     CDS             complement(2835..3113)
                     /locus_tag="VGB88_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9645878.1"
                     /translation="MIRRFRSRALKRLHERGEASQLNPEHIAKLRDILVRLEASAEAQ
                     DMDLPGFRVHRLTGNYRGYWAVVVRANWRVIFRFEAGDAVDVDYLDYH"
     gene            complement(3247..>4130)
                     /locus_tag="VGB88_00165"
     CDS             complement(3247..>4130)
                     /locus_tag="VGB88_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9645879.1"
                     /translation="SEIRPDIISCQVSAWGGPDGGALKDDPAFDPTLQSASGITARYG
                     SPDSPVIHAVASCVDYITGFTAALGIVQALIARDAGHGGAHVRSSLAMGAQLVQFPFM
                     VDHDGATHGTEASGQDATGEGAHHRLYRVADGWAFLGCRAEDTGRLSLALGANADGED
                     AIAERARTLAFEDFAAQVATVPSACAVRVASLAEVRRAQTVEADATAPVEIDGGSLRM
                     VRFAHPSGYTTTLPLPTWLRPERTPVRRLFPAPLPGTHTREVLGEAGLSAGEIDALFA
                     CGAAADHWPALSRYLPL"
BASE COUNT          547 a         1516 c         1473 g          594 t
ORIGIN      
        1 gacctgcccg agggtgccgc gttcattccc gagatgccgg gcaccgcgcc cggcctggtg
       61 tgcccgctcg gcgcgcaggt gatctatgcg gtgccgggcg tgccgcacga gatgcggacc
      121 atggtcggcg ccaccgtgct cgccgacctc aagcggcgcg cgggcctgcg cgcggtgatc
      181 cggagccgcg tgctgcgcac ctggggccac accgagtccg gcctcgccga gctgctcgcc
      241 gggcgcatcg ccgagctcga tgcgctcggc aacccgaccc ttgccttcca ggcgagcggc
      301 atcgaggggc tcaaggtgcg gatcaccgcc aaggcgggcg atgacgccgc cgccgaggcg
      361 atgcttgcgg acgaggaggc gcgggtgcgc gcgctggtcg gcgacgtggt gttcggcgtc
      421 gacggcgaga ccatggaatc ggtggtgctc gccatgctgg ctgcgcgcgg gctcagcctc
      481 gcggttgccg agcagctcag cgccgggctg gtggcgatgc ggctcacggc cgccgccaac
      541 ggcgcggacg gcaccttccg cggtggcgtg gtcaccgccg ccgacgggcg cgcgagcgag
      601 cttttggggg cgccgccggg gccggcggtc tcggccgagg ccgcggcggc gatggccgag
      661 cgcgtgcgcg cgctgttcgg caccgacgtc ggcctcgcca cctgcggcgt cgccggcccc
      721 gagccgcagg acggccaccg ccccggcacc gcgttcctcg gcctcgccct cggcaaccgc
      781 gccgaggcga ccgaggcgcg cctgcccggc gaccgcgacc gcatccgcca gtacgcggtg
      841 atcagcgtgc tcaatgcgct ccgccgcagg cttgccgccg cgcagtagcc gccacgacca
      901 ccagggagag ccgagaccga tgagccgcat tccgaccgtc gcccccaaag acatgaccga
      961 cgcccagcgc cgggtggtcg aggcgatccg ctccggcccc cgcggcagcg ccgcgcgcgg
     1021 cccgttcgca gcctggctcg cgagccccga gctcgccgac cgggcgcagc acctcggcgc
     1081 ctatctcagg ttcgagacct cggtgccggc gcggctcaag gagctcgcca tcctctatgt
     1141 cgcgcgcgcc tggaccgccc agttcgagtg gtacgcgcac aagaggttcg cgctcgacgg
     1201 cggcctcggc ccggaggtga tcgacgccat ccaggcgcgc cgccgccccg acttcacacg
     1261 cgacgacgag gccgcggtct acgatttctg tgcggagctg cacgagcgcc accaggtcag
     1321 cgacgcgcgc ttccaggcgg tcaagaaaca tctcggccag gcgggcgtgg tcgagctggt
     1381 gggtgtgctc ggctactaca cactggtgtc gatgaccttg aacgtgttcg aggtggcgat
     1441 gcccgagggc gagccgccgc cgcttgccga ctgagcgcat gattggcgcc gctgcgatgc
     1501 gcccccctca cccatccctg gatttaatcc ggggaccggg taaggcgcgg ctttcgccgc
     1561 gccaaccctt cgcaaccctc tccccccgcc aatcgcgggg ggagagggaa gggtgagggc
     1621 ggaccgagac aaggcaatcc gaaacccgtc gaaacggtcg cagacatggt ggccccgctg
     1681 acgccgcgcg ccgcagcggt gcgcgccgcg ctgtggatgt gcggcacgct cgtctcgttc
     1741 agcatcatgg cggtggcgat ccgcgagctg tcggcgagcc agggcacgtt ccagatcctc
     1801 gcctggcgca gcatcgtcgg cctcgccgtg ctggtgccgg tcgcgctgct gcgcgggcgc
     1861 gggctgctgg ccacccgcca gctgcgctgg caggtgcggc gcaacctggt ccacttcctc
     1921 ggccaggcgg gctggaccta cggcatcgcg gtgctgccgt tgactgtcgt gttcacgctc
     1981 gagttcacca tgccggcgtg gaccgcgctc ttggcggtgc tgttcctcgg cgagcggctg
     2041 agccggggcc gcgcgctcgc catcgccggc ggcatcgccg gcgtggtcgt gatcctgcgc
     2101 cccgatgtcg cgctgcccga tccggccgcg ctcatcgtgc tcgcggcggc catggcctac
     2161 gccgcggcgc acaccatgac caagcagctc acccgcagcg acggcgcgct cgcgatcctg
     2221 ttctggatgt cggcgatcca gctgccgctc ggcgcgggcg tggcgctggc gctcgcgggc
     2281 ggcgactggc cgctgccggg gtgggtcgcg gcgccatgga tcgcgctggt cgggctcgcc
     2341 gcgctcgccg cccatttctg cctcgcgcgc gcgctcaagc tcgccgacgc gactgtggtc
     2401 atgccgctcg acttcctgcg cctgccgctg atcgcaggcg tgggcgcggt gctctacggc
     2461 gaggcgccca cgcccgcggt cgcgctcggt gcggcgatca tcgtcttgag cctcgcgttc
     2521 gcgctccgcg ccgagcgcta ggccgcgcgg aacgacacca ccttaatgcc cgccgccttc
     2581 cttccggcgt gccacaggtc gtactgcatc tgttgtctga gccagctctc ggcgctaccg
     2641 ccgaacgcct tcgacaatcg gatcgccatc tccggcgaga tgctgaggcg gccgttgagc
     2701 agcatcgaca gggtgttgcg cgacactccg agacccttcg cggccgcggt cacggtcaac
     2761 cccaacggct ccaggcactg gcgtcggatc accgcgccgg gatgcggcgg atcgtgcatc
     2821 ggcatcggcc cctcctaatg atagtcgaga taatccacgt cgaccgcgtc gcccgcctcg
     2881 aagcggaaga tcacgcgcca attggctctg accaccaccg cccagtaacc tctgtagttg
     2941 ccggtcaggc ggtgaacgcg aaagccgggc agatccatgt cctgcgcttc cgccgacgcc
     3001 tccaggcgaa cgaggatgtc ccgcagcttc gcgatatgct ccgggttcag ctggctcgcc
     3061 tcgccgcgct catggagccg tttcagcgcc ctgctgcgga aacgccggat cacgacgagc
     3121 ttgtatcatg taataataca tttgtcgcgg cgcaactcgg caacgacagt tgtatgatcg
     3181 ccgaatcgga gcaagacgct cggcgaccgg cgccgccgac gactcgcggt gcaagtcgca
     3241 cggcgatcac aacggcaggt agcgcgacag tgcaggccag tggtccgccg cggcaccgca
     3301 ggcgaacagc gcgtcgatct cgcccgcgct caagcccgcc tcgcccaaca cctcgcgggt
     3361 gtgggtgccg gggagaggcg ccgggaacag gcgccgcacc ggagtcctct cgggtcgcag
     3421 ccaggtcggc agcggcaagg tggtggtgta gcccgagggg tgggcgaagc gcaccatcct
     3481 gagcgagccg ccgtcgatct cgaccggcgc ggtggcgtcg gcctcgaccg tctgcgcgcg
     3541 ccgcacctcg gcgagcgagg ccacccgcac cgcgcaggcc gacggcaccg tcgccacctg
     3601 cgcggcgaag tcctcgaacg ccagcgtgcg cgcgcgctcg gcgatcgcat cctcgccgtc
     3661 ggcattggcg ccaagcgcca ggctgagcct gccggtgtcc tccgcgcggc agccgaggaa
     3721 cgcccagccg tcggccaccc ggtagaggcg gtggtgggcg ccctcgccgg tcgcgtcctg
     3781 gccgctcgcc tcggtgccgt gggtggcgcc gtcgtggtcg accatgaacg ggaactggac
     3841 gagctgcgcg cccatcgcga gcgagctgcg cacgtgggcg ccgccgtgcc cggcgtcgcg
     3901 cgcgatcagc gcctggacga tgccgagcgc ggcggtaaac ccggtgatgt agtcgacgca
     3961 gctcgccacc gcgtggatca ccggcgagtc gggcgagccg tagcgcgcgg tgatgccgct
     4021 cgccgactgc agcgtcgggt cgaacgccgg gtcgtccttg agcgcgccgc cgtccgggcc
     4081 gccccaggcg ctgacctggc acgagatgat gtcgggcctg atctcgctca
//
LOCUS       DASWHU010000006         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2589404, whole genome shotgun sequence.
ACCESSION   DASWHU010000006 DASWHU010000000
VERSION     DASWHU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589404"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VGB88_00170"
     CDS             <1..453
                     /locus_tag="VGB88_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HEX9645880.1"
                     /translation="TRGRLIRLAYAIDMRYGVLHEIASRVRAGEPVDLATGHLSLIWQ
                     GDAGAQILRSFAHCATPPRPLNITAREIASVRALALAFGRRFDVEPVFRNREAETAWV
                     MDGAEAARLFGAPIVSTETMIDWTADWVARGQPSYGRPTRYEVRDGRF"
     gene            470..1300
                     /locus_tag="VGB88_00175"
     CDS             470..1300
                     /locus_tag="VGB88_00175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9645881.1"
                     /translation="MRALVPSDAGRLVALSAEAGWNQTADDWRLMLELGEGIGCWEPD
                     GAPIASALVLPYDGGFAWLSMVLVTAPWRRRGLATRLVRRCLDRLAEIGVSPILDATE
                     AGRRTYSRLGFTDLWTYTRLAAERPRLAPAAGIDVRPMTPTDLDAVAALEARAFGAPR
                     KHVLAAMLARAPDAAYLAESGGRLCGLVLARDGRLAIQLGPLVAETPEVARGLAAAAL
                     AGTGGPVFVDLPDAQGAFRAALEGAGFAAQRGLVRMAAGRGDPGEASLRYALAGPELG
                     "
     gene            1373..2431
                     /locus_tag="VGB88_00180"
     CDS             1373..2431
                     /locus_tag="VGB88_00180"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9645882.1"
                     /translation="MATSADIPEGVRAVLTAGTVIPAHPLALDAARRLDRHRQRALAR
                     YYLDAGAGGLAVGVHATQFQIRDAGLYVPVLEIAAETAAAWSDRPVAMIAGIAGRTNQ
                     AVVEAQNARTLGYHAGMVSLSAFRGASEDEMIAHCARIGRELPIVGFYLQPAAGGVPL
                     SAAFWKRFAAIESVVAIKIAPFNRYRTIDVIRGVVEAAAEDRITLYTGNDDHIVLDLV
                     TPFGAKRDGAEVRVRIRGGLLGQWGVWTRTAVRLFERLRAAAEALCVDAELLALDWKL
                     TDANQAIFDVANDFRGCIPGCHEILCRQGLLAGIWCLDPSETLSPGQADEIDRVCRSY
                     PELADDDFVTGNLDRWLA"
     gene            2447..>2752
                     /locus_tag="VGB88_00185"
     CDS             2447..>2752
                     /locus_tag="VGB88_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9645883.1"
                     /translation="MAETRLGIIMHGVTGRMGANQHLARSIAAIRAEGGVALADGSRV
                     MPEPVLVGRDEAKLAALARAHGVERWTTDLDSALGDDSLTVFFDAASTHLRAGLLIKA
                     "
BASE COUNT          360 a         1011 c          994 g          387 t
ORIGIN      
        1 acgcgaggcc ggctgatccg cctggcctac gccatcgaca tgcgctacgg cgtgctccac
       61 gagatcgcga gccgggtgcg cgcgggcgag cccgtcgacc ttgccaccgg ccacctgtcg
      121 ctgatctggc agggcgacgc cggcgcccag atcctgcgca gcttcgcaca ttgcgcgacc
      181 ccgccccggc cgctcaacat caccgcgcgc gagatcgcca gcgtgcgcgc gctcgccctt
      241 gccttcggcc ggaggttcga cgtcgagccc gtgttccgga accgcgaggc cgagaccgcc
      301 tgggtgatgg acggcgcgga agccgcgcgc ctgttcggcg cgccgatcgt atccaccgag
      361 acgatgatcg actggaccgc cgattgggtc gcacgcgggc aaccctctta tggcaggccg
      421 acccgctatg aggttcgcga cggacgcttc tgacccggcg cgcctgggga tgcgcgcgct
      481 tgtgccgagc gatgccgggc gtctggtggc gctgtccgcc gaggccggct ggaaccagac
      541 cgccgacgac tggcggctga tgctcgagct cggcgaggga attggatgct gggagccgga
      601 cggggcgccg atcgcctcgg cactggtgct gccctacgat ggcggcttcg cctggctgag
      661 catggtgctg gtgacggccc cctggcggcg gcgcgggctc gcgacacggc tcgttcgccg
      721 ctgcctcgac cggctggccg aaatcggcgt gtcgccgatc ctcgatgcca ccgaggcggg
      781 ccggcgcacc tactcccgcc tcggcttcac cgatctgtgg acctataccc ggctcgcagc
      841 cgagagacct cggctcgccc cggcggcggg gatcgacgtg cgcccgatga cgcccacaga
      901 cctcgacgcg gtggccgcgc ttgaggcgcg cgcgttcggc gctccgcgga agcacgtgct
      961 ggcggcgatg ctcgcacgcg cgcccgatgc ggcgtacctc gccgagtccg gcggccggct
     1021 ctgcggactg gtgctcgccc gcgacggccg gctcgccatc cagctcgggc cgctggtcgc
     1081 cgagacgccc gaggtcgcgc gcggacttgc cgccgcggca ctcgcgggta cgggcgggcc
     1141 ggtgttcgtc gacctgcccg atgcccaggg cgcattccgc gccgcgctcg agggcgccgg
     1201 cttcgccgcc cagcgcggcc ttgtgcgcat ggccgcaggc cgcggcgacc cgggcgaggc
     1261 gtcgctccgc tacgccctcg ccggtcccga gctcggctga ccggcttgac cgcgatcgcg
     1321 tccggccact atcaccaccg gaatggacca acggcagccg aggcgggcac aggtggcgac
     1381 aagtgccgac atcccggagg gcgtacgcgc ggtgctcacg gcgggcacgg tgatcccggc
     1441 ccatccgctg gcgcttgacg ccgcgcgccg gctcgaccgg caccgccagc gcgcgcttgc
     1501 ccgctactac ctcgacgccg gcgctggcgg gctcgcagtc ggcgtgcacg cgacccagtt
     1561 ccagatccgc gacgccgggc tgtacgttcc cgtgctcgag atcgccgccg agaccgcagc
     1621 cgcgtggagc gaccggccgg tggcgatgat tgccggcatc gccgggcgca ccaaccaggc
     1681 ggtggtcgag gcccagaacg cgcgcaccct cggctaccac gccggcatgg tcagcctgag
     1741 cgcgttccgc ggcgcgagcg aggacgagat gatcgcgcat tgcgcgcgca tcgggcgcga
     1801 gctgccgatc gtcggcttct acctgcagcc ggcggcgggc ggggtgccgc tgtcggcggc
     1861 gttctggaag cgcttcgccg cgatcgagtc ggtggtcgcg atcaagatcg ctccgttcaa
     1921 ccgctaccgc accattgacg tgatccgcgg cgtggtcgag gccgccgccg aggaccgcat
     1981 cacgctgtac acgggcaacg acgaccacat cgtgctcgac ctggtcacac cgttcggcgc
     2041 caagcgcgac ggcgcggagg tgcgggtgcg aatccgcggc gggctgctcg gccaatgggg
     2101 ggtatggacg cgcaccgcgg tgcggttgtt cgagcgcctg cgtgcggcgg cggaggcgct
     2161 gtgcgtggac gccgagctgc tcgcgctcga ctggaagctg accgacgcca accaggcgat
     2221 cttcgacgtc gccaacgact tccgcggctg catcccgggc tgccacgaga tcctgtgccg
     2281 ccagggattg ctcgccggca tctggtgcct cgaccccagc gagaccctga gccccggcca
     2341 ggccgacgag atcgaccgcg tctgccgcag ctaccccgag ctcgccgacg acgactttgt
     2401 caccggtaac ctcgaccgct ggctggcctg accgtgggtc gaagcgatgg ctgagactcg
     2461 gcttggcatc atcatgcacg gggtgaccgg gcgtatgggc gcgaaccagc acctcgcgcg
     2521 ctcgatcgcc gccatccgtg ccgagggcgg ggtggcgctc gccgacggca gccgggtgat
     2581 gcccgagccg gtgctggtcg gacgcgatga ggcgaagctc gcggcactcg cccgcgccca
     2641 cggcgtcgag cgctggacca ccgacctcga ctcagcgctc ggcgacgact cgctcacggt
     2701 gttcttcgac gccgcatcga cgcatctgcg cgccggcctg ctgatcaagg cg
//
LOCUS       DASWHU010000007         6434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_741505, whole genome shotgun sequence.
ACCESSION   DASWHU010000007 DASWHU010000000
VERSION     DASWHU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741505"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1723)
                     /locus_tag="VGB88_00190"
     CDS             complement(17..1723)
                     /locus_tag="VGB88_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9645884.1"
                     /translation="MRKLATLGAMLAATGAAAVLALAATTSLAQEGTLYGDMKTVSQD
                     MMNRAVGDANNFLHTNGNYNQTRFYPAAQINADNVGSLRPAWIFQTEVVETMETSPLV
                     VDGVMYATTAFSHVYALDAATGAELWHYKHKMGPVTTYCCGPNNRGVAAYGDMVYLGT
                     LDAKLVALDAKTGALKWEKQIADPELGYSETMAPTAVNGKILIGTNGGEYGIRGFVKA
                     FDAKSGDLLWTFHTIPENSVGVWATHDATGRDMLRDIAAEKKALAALGDPYKTLGGGV
                     WQNPAVDLGMNRIFFSVGNPSPDLDGAIRPGDNLYTDSLVSVNLDTGEYACHFQYVAH
                     DVWDLDAVSPPILVDVMDENGDVVPGLIHGGKTGHIYVHDRRDCRLIRFSEAMVPQEN
                     MWVLPTAEGARMLPGANGGVEWSTMAVNAGLGLAYAINLHQPMTYHVESTPYPGGKLW
                     LGGAFKVIASEEQWGNVTAVDYNTGKIAWQVKTPQPMIGGVLATAGGLVFTGEGNGLF
                     KAYDAASGNVLWQFQAGAGVNAPPVSYTVGGTQYIAVAAGGNTQLNFKRGNNIIAFSL
                     AE"
     gene            1952..2671
                     /locus_tag="VGB88_00195"
     CDS             1952..2671
                     /locus_tag="VGB88_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX9645885.1"
                     /translation="MRLSGFQALSFDCYGTLIDWEAGLLAALGPWRGRTRPRGDDEAL
                     LAAFARHESAQQAETPGLGYREVLARVLHRIAREVEVNLRADEARAFAASIGAWPAFP
                     DSPAALRYLKQHYTLVVLSNVDRASFAASEAALGVSFDAVYTAEDIGSYKPDARNFAY
                     LVARMAERGIAATRLLHVAQSLYHDHVPAKAAGLATAWIDRRAGRPGGATVLPGGPVS
                     YDFRFATLGDLADAHRAEIGR"
     gene            2738..3823
                     /locus_tag="VGB88_00200"
     CDS             2738..3823
                     /locus_tag="VGB88_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9645886.1"
                     /translation="MAAEFIPQFAPRDKPVAPFSMLGANGRLKRGAEPELDDRATLEA
                     LRLMMLGRMFDQKCLSLQRQGRLSTFAPIFGQEATLAGTAMAMDPARDWAVPQYREAP
                     VQIRQGLPLATLALFRRGHPDGGVIPAGVNVLATQVSLAAQLPHAVGLAWGIRMKGGD
                     GVVVVFFGDGASSEGDFHESCNLAGLVDAPVIFLLQNNQWAISTPRAIQSAARDLAAR
                     APGYGFAGVSVDGNDLLAMHKVAADAVRRARAGGGPTLIEAHTYRIGMHTTADDPSRY
                     VDAGELERWKKRDPIERVQRYLAAKGKWDDARAAAWEDEIRAEIEAAFDHADGYPPPR
                     PEQIYEHLYAEPTPTLIRQRRANLGQP"
     gene            3834..4814
                     /locus_tag="VGB88_00205"
     CDS             3834..4814
                     /locus_tag="VGB88_00205"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9645887.1"
                     /translation="MPRMNMVEAIRGALKQEMERDERVILMGEDIGHLGGVFRVTDGL
                     QQFFGSKRVIDTPLAESVIAGSAMGLAIAGWVPVVEIQFLGFAQQCFHQICQQVARIR
                     YRTRGSHTAPVTIRAPFGGNVRTPEFHSDSMEQTFVQACGLKVVMPSNPYDAKGLLAQ
                     AIRDPDPVMYLEPLRGYRLVSGEVPHDAYLVPFGELRTAREGDDVTVVAWSAAVQVAE
                     KAAELAAEKGISCHVVDLRTLVPLDEAGLIAAVERTGRCVVVHESPAVAGFGAEVIAL
                     LQEQAFYSLEAPMRRVTAPDVPYPMTGIEDDYVPNAARVLAAIEQTVAGL"
     gene            4811..6028
                     /locus_tag="VGB88_00210"
     CDS             4811..6028
                     /locus_tag="VGB88_00210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9645888.1"
                     /translation="MSAFQFNLPDVGEGLSEGEIVRWHVAPGDAVTADQIIVDVQTDK
                     AVVEIPAPVGGTVRTLGGEVGDVVPVGALLAVIETDGAARAPAREAETRPEPKPAAAA
                     PPPAAARSARVLASPATRKLAAELGVDLAAVPGTGNRGQVTRDDVRRAGEGGAPTPSA
                     ARPRRAAVLAPEGEDRIEPLRGLRRQIAIAVAAAWRDIPHILTAHQIDAESLIAARRA
                     LNEEYGGEGVKLSYLPLFVKACVAALRRHPRFNASLDMDKGEIVYRHRCNIGIATATP
                     DGLIVPVVHDADRKSILDIAREIEALAAAARERKITVEQLQGGTFTITNYGSYGATFG
                     TPIIRQPEVAIAGFGAIQDMVVPVDRQPVVRPILPLVVSTDHRLNDGEHLGQFVDSLS
                     AYLTDPVRLLGAM"
     gene            6028..>6434
                     /locus_tag="VGB88_00215"
     CDS             6028..>6434
                     /locus_tag="VGB88_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9645889.1"
                     /translation="MVVGQIEDAVDFLVVGAGPGGYVAALRASQLGREVTLIDRDGAE
                     GVGGVCLRVGCIPSKALIEVADLAHRAREAKAFGLEASFGAFDMARFQAWKGEVVTGL
                     TDGVRALLKAAKVTVAAGEFRLTGPDSGVLTPA"
BASE COUNT          913 a         2243 c         2310 g          968 t
ORIGIN      
        1 gctcagcgcg tgcgcgtcac tcggcgagcg agaacgcgat gatgttgttg ccgcgcttga
       61 agttgagctg ggtgttgccg ccggcggcga ccgcgatgta ctgcgtgccg cccacggtgt
      121 aggagaccgg cggcgcattg acgccggcac cggcctggaa ctgccacagc acgttgccgc
      181 tggccgcgtc ataggccttg aacaggccgt tgccctcgcc ggtgaagacc aggccgccgg
      241 cggtcgcgag cacgccgccg atcatcggct gcggggtctt cacctgccag gcgatcttgc
      301 ccgtattgta gtcgacggct gtgacgttgc cccactgctc ctcgctcgcg atcaccttga
      361 acgcgccgcc cagccacagc ttgccgcccg gatagggcgt gctttcgacg tggtaggtca
      421 tcggctggtg caggttgatc gcgtaagcga ggccgaggcc cgcattgacc gccatggtcg
      481 accactcgac gccgccattg gcgccgggca gcatgcgcgc gccctcggcg gtgggcagca
      541 cccacatgtt ctcctgcggc accatcgcct cggagaagcg gatcaggcgg cagtcgcgcc
      601 ggtcgtggac atagatatgg ccggtcttgc cgccgtggat caggccgggc accacgtcgc
      661 cgttctcgtc catcacgtcg accaggatcg gcgggctgac tgcgtccagg tcccacacat
      721 catgggcgac gtactggaag tggcaggcat actcgccggt atccaggttc accgacacca
      781 gcgagtcggt gtacaggttg tcgcccgggc ggatcgcgcc gtcgaggtcg ggcgacgggt
      841 tgccgaccga gaagaagatg cggttcatgc cgaggtcgac cgcggggttc tgccacacgc
      901 cgccgccgag cgtcttgtac gggtcgccga gcgcggccag cgccttcttc tcggccgcga
      961 tgtcgcgcag catgtcgcgt ccggtggcgt cgtgggtggc ccacacgccg accgagttct
     1021 cggggatggt gtggaacgtc cacagcaggt cgccggactt ggcgtcgaac gccttgacga
     1081 agccgcggat gccgtactcg ccgccattgg tgccgatcag gatcttgccg ttgaccgcgg
     1141 tcggcgccat ggtctcgctg tagccgagct cgggatcggc gatctgcttc tcccacttga
     1201 gcgcgccggt cttggcgtcg agcgcgacca gcttggcgtc gagcgtgccc aggtagacca
     1261 tgtcgccgta ggcggcgacg ccgcggttgt tgggaccgca gcaataggtg gtgaccggcc
     1321 ccatcttgtg cttgtagtgc cacagctcgg ccccggtcgc ggcatcgagg gcgtagacgt
     1381 ggctgaacgc cgtggtcgcg tacatcacgc cgtcgaccac cagcggcgag gtctccatcg
     1441 tctccaccac ctcggtctgg aagatccagg ccgggcgcag gctgccgacg ttgtcggcgt
     1501 tgatctgcgc cgccggatag aagcgggtct ggttgtagtt gccgttggtg tgcaggaagt
     1561 tgttggcgtc gccgacggcg cggttcatca tgtcctgcga caccgtcttc atgtcgccgt
     1621 acagcgtgcc ctcctgggcc agcgatgtgg tggcggcgag cgcaagcacc gccgccgcgc
     1681 cggtcgccgc gagcatggcg ccgagcgtcg caagtttcct cattttgtgc cgtgcctccc
     1741 aaatgcctgt cttcgtggcc aggcgggacc aaggcgcggc cccctttgcc ggccctcggg
     1801 cagccgttgt ctccggcctt cccacccggg tcccccctag ggaccgatgc cggcgatagt
     1861 aggcatcgcg ggcacgggcg gcaacatcga aagcggcggt ccctggccga tcgaccgcag
     1921 ccgtgcgata tcgacccgag gaggatcgac catgcggttg agcgggttcc aggccttgag
     1981 cttcgactgc tacggcacgc tgatcgactg ggaggcgggc ctgctggcgg cgcttgggcc
     2041 gtggcgcggg cgcacgcgcc cccgtggcga cgacgaggcg ctgctcgccg cgttcgcgcg
     2101 ccatgagagc gcgcagcagg ccgagacccc ggggctcggc taccgcgagg tgctggcgcg
     2161 cgtgctgcac cgcatcgccc gggaggtcga ggtcaacctg cgcgccgacg aggcacgggc
     2221 gttcgccgcc tcgatcggcg cctggcccgc cttccccgac agtcccgcgg cgctccgcta
     2281 cctcaagcag cattacacat tggttgtgct gtccaacgtc gaccgggcga gcttcgccgc
     2341 cagcgaggcc gcgctcgggg tgagcttcga tgcggtctac accgccgagg acatcggctc
     2401 gtacaagccg gatgcgcgca acttcgccta tctggtcgcg cgcatggccg agcgcggcat
     2461 cgccgcaacc cggctcttgc acgtcgccca gagcctctat cacgaccacg tcccggccaa
     2521 ggccgcgggc ctcgccaccg cctggatcga ccgccgtgcg ggacgcccgg gcggcgccac
     2581 ggtgctgccg ggcggtccgg tgtcctacga ctttcgcttc gccacgctcg gcgacctcgc
     2641 cgacgcgcac cgcgccgaaa tcggccgctg acggcagtct tgcacgcgga cgcgcgcgct
     2701 tccacctgtc gaacgccgat gctaggttgg cgccgcgatg gccgctgagt tcatccccca
     2761 gttcgcgccg cgggacaagc ccgtggcgcc gttcagcatg ctcggcgcga acgggcggct
     2821 gaagcgcggc gccgagcccg agctcgacga ccgggcgacg ctggaagcgc tcaggctgat
     2881 gatgctgggg cggatgttcg accagaaatg cctgagcctc cagcgccagg ggcggctgtc
     2941 gaccttcgcg ccgatctttg gccaggaggc gacgctcgcc ggcactgcga tggcaatgga
     3001 cccggcgcgc gactgggcgg tgccgcaata tcgcgaggcg ccggtgcaga ttcgccaggg
     3061 gctgccgctc gccaccctgg ctttgttccg ccgcggccac cccgacggcg gcgtgattcc
     3121 ggccggcgtc aacgtgctcg ccacccaggt ctcgctcgcc gcgcaactgc cgcacgcggt
     3181 cggcctcgcc tggggcatca ggatgaaggg cggggacggc gttgtggtgg tcttcttcgg
     3241 cgacggcgcg tcgtccgagg gcgacttcca cgagtcctgc aacctcgccg ggctggtcga
     3301 tgcgccggtg atcttcctgc tgcagaacaa ccagtgggcg atctcgaccc cgcgcgcgat
     3361 ccagagcgcc gcgcgcgacc tcgccgcgcg cgcgccgggc tacggtttcg ccggcgtctc
     3421 ggtcgacggc aacgacctgc tggcgatgca caaggtggcg gcggatgcgg tccggcgcgc
     3481 ccgcgccggc ggcgggccga ccctgatcga agcgcacacc taccggatag gcatgcacac
     3541 caccgccgac gacccgagcc gctacgtcga cgcgggcgag ctcgagcggt ggaagaaacg
     3601 cgatccgatc gagcgcgtcc agcgttacct cgccgccaag ggcaagtggg acgatgcgcg
     3661 cgccgccgcc tgggaggacg agatccgcgc cgagatcgag gccgcgttcg accatgccga
     3721 cggctatccg ccgcctcggc cggagcagat ctacgagcat ctctacgccg agccgacgcc
     3781 gacgctgatc cgccagcgcc gcgccaatct gggccagcca tgaggtgcgc gcgatgccca
     3841 ggatgaacat ggtcgaggcc atccgcggcg cgctcaagca ggagatggag cgcgacgagc
     3901 gggtgatcct gatgggcgag gacatcggcc atctgggcgg ggtgttcagg gtcaccgacg
     3961 ggctgcagca gttcttcggc tcgaagcggg tgatcgacac cccgctcgcc gaaagcgtca
     4021 tcgcaggctc cgcgatgggg ctggccatcg ccggctgggt gccggtggtc gagatccagt
     4081 tccttggctt cgcccagcag tgcttccacc agatctgcca gcaggtcgcg cgcatccgct
     4141 accgcacgcg cggctcgcat accgcgccgg tcaccatccg cgcgccgttc ggcggcaacg
     4201 tgcgcacgcc cgagttccac tccgattcga tggagcagac cttcgtccag gcctgcggcc
     4261 tgaaggtggt gatgccgtcc aacccgtacg acgccaaggg cctgctggcc caggcgatcc
     4321 gcgacccgga cccggtcatg tacctggaac cgttgcgcgg ctaccggttg gtgtcgggcg
     4381 aggtgccgca cgacgcctac ctggtgccgt tcggcgagct gcgcacggcg cgcgagggcg
     4441 acgacgtcac cgtggtcgcg tggagcgcgg cggtgcaggt ggcggagaag gcggcggagc
     4501 tagccgcgga gaagggcatc agctgccacg tcgtcgacct gcgcaccttg gtgccgctcg
     4561 acgaggccgg gctgatcgcg gcggtcgagc gcaccgggcg ctgcgtcgtg gtccacgaat
     4621 cgccggcggt ggcggggttc ggcgccgagg tgatcgcgct gttgcaggag caggcgttct
     4681 attcgctgga ggcgccgatg cgccgggtca ccgcacccga tgtcccctac ccgatgaccg
     4741 gaatcgagga cgactacgtg cccaacgcgg cgcgcgtgct ggccgcgatc gagcagacgg
     4801 tggcggggct gtgagcgcgt tccagttcaa cctgcccgac gtgggcgagg gcctgagcga
     4861 gggcgagatc gtccgctggc atgtcgcgcc cggcgatgcg gtcacggccg accagatcat
     4921 cgtcgacgtc cagaccgaca aggcggtggt cgagattccg gcgccggtcg gcggcacggt
     4981 caggacgctc ggcggcgagg tcggcgacgt cgtgccggtg ggcgcgctgc tggcggtgat
     5041 cgagaccgac ggtgccgcgc gtgcaccggc cagggaggcg gagactcggc cggagccaaa
     5101 gcccgctgcg gccgccccgc cgccggcggc ggccaggtcg gcgcgcgtgc tggcgagccc
     5161 ggcgacgcgc aagctcgcgg ccgagctcgg cgtcgacctc gccgccgtcc ccggcaccgg
     5221 caaccgcggc caggtgaccc gcgacgacgt gcggcgggcc ggcgagggcg gcgcgcccac
     5281 gccgtcggcg gcgaggcccc ggcgcgcggc cgtgctcgcg cccgagggcg aggaccgcat
     5341 cgagccgttg cgagggctca ggcgccagat cgcgatcgcc gtggccgccg cctggcgcga
     5401 catcccgcac atcctgaccg cgcaccagat cgacgccgaa agcctgatcg cggcgcgccg
     5461 cgcgctgaac gaggagtatg gcggcgaagg agtcaagctc agctacctgc cgctgttcgt
     5521 caaggcgtgc gtcgccgcgc tcaggcgcca tccgcgcttc aacgcctccc tcgacatgga
     5581 caagggcgag atcgtctacc gccaccgctg caacatcggc atcgccaccg ccactcccga
     5641 cggactgatc gtgccggtgg tccacgacgc cgatcgcaag tcgatcctcg acatcgcgcg
     5701 cgagatcgag gcgctggccg cggccgcgcg tgagcgcaag atcaccgtcg agcagctcca
     5761 gggcggcacc ttcaccatca ccaactacgg cagctacggg gcaacctttg gcacgccgat
     5821 cattcgccag ccagaggtcg ccatagcagg gttcggcgcg atccaggaca tggtggtgcc
     5881 ggtggaccgc caacctgtgg tgcggccgat cctgccgctg gtagtgtcga ccgaccaccg
     5941 cctcaacgac ggcgagcatc tgggccagtt cgtcgacagc ttgagcgcct acctcaccga
     6001 cccggtgcgc ctgctcgggg cgatgtgatg gtcgtcggcc agatcgagga cgcggtcgac
     6061 ttcctggtgg tcggcgccgg ccccggcggc tacgtggcgg cgctgcgcgc ctcacagctc
     6121 ggccgcgagg tgacgctcat cgaccgcgac ggcgccgagg gcgtgggcgg ggtgtgcctg
     6181 cgcgtcggct gcatcccgag caaggcgctg atcgaggtcg ccgacctcgc gcatcgcgcg
     6241 cgcgaggcca aggcgttcgg cctcgaggcc tcgttcgggg ccttcgacat ggcgcgtttc
     6301 caggcgtgga agggggaggt cgtcacgggc ctcaccgacg gcgtgcgcgc cctcctcaag
     6361 gcggcgaagg tcacggtcgc ggcgggcgag ttccgcctta ccgggcccga ctcgggcgtg
     6421 ctcacgccgg cgga
//
LOCUS       DASWHU010000008        13817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1111018, whole genome shotgun sequence.
ACCESSION   DASWHU010000008 DASWHU010000000
VERSION     DASWHU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111018"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..425
                     /locus_tag="VGB88_00220"
                     /pseudo
     CDS             162..425
                     /locus_tag="VGB88_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(402..623)
                     /locus_tag="VGB88_00225"
                     /pseudo
     CDS             complement(402..623)
                     /locus_tag="VGB88_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            852..4277
                     /locus_tag="VGB88_00230"
     CDS             852..4277
                     /locus_tag="VGB88_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009816264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX9645890.1"
                     /translation="MTVAIAPISRQIWDMKYRLRAPSGEPLDGTVEDSWRRIATALAV
                     PEADPAAWQGRFYEALEDFQFLPAGRIVAGAGSKRKVTLFNCFVMGPVADDMAGIFES
                     LKEAALTMQQGGGIGIDFSTLRPRGAAVHGVGADASGPISFMRVWDAMCRTIMSAGHR
                     RGAMMATLCCDHPDIEAFIDAKREPGELRMFNLSVLVSDAFMDAIRRDAPWDLAFGGT
                     TWRTLPARELWQRIMRATYAYAEPGVVFIDRVNRLNNLSYCEEIHATNPCVTADTWVQ
                     TAAGPAQVADLVGRPFTAVVDGQSCPSGADGFFATGRKPVLRLVTAAGHTLRLTADHP
                     VLRVTRRTRWHEQTDWCPAGALRPADHIRLGNQRPAADWPGACSEDQGYLIGLLIGDG
                     TLTADKAVLSAWPGAEAVHGGVERPGVLAVMERALTAARSLPHRSDFAGWMAVPGRGE
                     HRLALGAIRALAIELGLRPGAKGVTPALERSSAAFHRGLLRGLFDADGSVQGSQSKGV
                     SVRLAQSDLEILRAVQRMLLRLGIAGRIYAERRPAGPRRLPDGRGGTQLYDCAADHEL
                     VIAGDNLQRFAERIGFADTDKAAALGRALAAYRRRLNRERFTAVVAAVEPDGEAEVYD
                     VRIPDRNAFDANGLIVHNCGEQPLPPYGACLLGSINLASLVGEPFTDQAALDLERLDR
                     LVPVAVRMLDNAIDVSRYPLPRQAEEARAKRRIGLGITGLADALIMCGARYGSARAVA
                     LTETWMERFQRAAYLASAALAAEKGAFPLYDADKYLAGEYVAGLPDELRQAIAADGMR
                     NGLVTSVAPTGTISLMADNVSSGIEPVYAFAHRRNVLMPDGTTQSTEVVDHAARLYRE
                     TFGENAPLPKSFVDVQALTPADHLVMEATVQRYIDSSISKTINCPEAISFADFESIYQ
                     RAYELGCKGCTTYRPNVITGAVLETEAEAAARAAPGAQAELALEPLAVRPGDEYDAGA
                     VVYMTRPLDRPEELPGTTYKLRWPESEHALYVTINDIVKDGRRRPFEIFVNSKNLEHY
                     AWTMGLTRMISAVFRRGGDVSFVVDELKAVFDPRGGQWLGGHYVPSLLAAIGEVVERH
                     MIEIGFLKTEAARGAATGAAAGALRACPRCGRRALIRQEDCDTCTACGYSHCD"
     gene            complement(4290..4832)
                     /locus_tag="VGB88_00235"
     CDS             complement(4290..4832)
                     /locus_tag="VGB88_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX9645891.1"
                     /translation="MNDLWVFAYGSLMWQPGFDYLDAQPALLRGYHRAFCVYSHIYRG
                     TPERPGLVLGLDRGGSCRGTAYRVHRSQAGEVLDYLTEREMRTSVYHPRFLAMTVPGR
                     RIRAHTFVVRRGHRQYAGPLPIARLVALVLQGEGTKGRCRDYLERTVCHLRERACLEP
                     RLERLHGHVLAAERLRSAAG"
     gene            4887..5936
                     /locus_tag="VGB88_00240"
     CDS             4887..5936
                     /locus_tag="VGB88_00240"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEX9645892.1"
                     /translation="MPRILGLYALLALAALGYTGYWFVLAERAEALVDAELAAWRADG
                     YAVSYTERRTSGYPYRLSTEVGDFTLVRDGVRWPWAWHGERLVVTLQPWDLGHYVATL
                     EGINRLDLPPSGDGESFALTAASARASLVLDGRYQAERGSAELQDAVLREALWPDGVT
                     IRRLQAHLRRPAEGPDTNPEASLDAVVQADALRLPDGAGGLLGREVALVYLEGTVIGP
                     LPGSFEPEPVALWRDSGGFIDARSLVLEWGPVRISAAGSLALDAAMRPLGALTGRIVG
                     FAALIDRLAAAGRMSAEAAAAAQLAFALIAKTPAGGGAPVLTVPLTLQDGWLFAGPVR
                     MVPLAPLFAAADLAG"
     gene            complement(5959..6846)
                     /locus_tag="VGB88_00245"
     CDS             complement(5959..6846)
                     /locus_tag="VGB88_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEX9645893.1"
                     /translation="MTETALFERIALIGIGLIGSSLARVIRAKGLARATAAYSRTAAT
                     RRRAAELGLVDQVIDDPAKAVAGADLVVIGVPVGASGEVAAAIAPGLAPGAIVTDVGS
                     VKGAVIEAVRAALPEGVAFVPGHPIAGTEHSGPDAGFAELFEGRWCVLTPTADTDRRA
                     VERVAALWRAAGSTVDLMSAEHHDTVLAITSHIPHLIAYNIVSTVADLEDRTKLEVIK
                     YAAGGFRDFTRIAASDPVMWRDVFLNNRTAVLEMLATFNEDLARLQRAIRRNEGEVLE
                     KIFTETRAIRRGVIEARQD"
     gene            complement(6863..7729)
                     /gene="pheA"
                     /locus_tag="VGB88_00250"
     CDS             complement(6863..7729)
                     /gene="pheA"
                     /locus_tag="VGB88_00250"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX9645894.1"
                     /translation="MSNDRSIDELRQEIDRIDDQIHDLLMRRTEIVGRIGAVKRQNEP
                     AVVAMRPGREAQIIRRLVARHSGPFPVEVLVRIWRELLSAQVSVQGDFSIGAFVPEGV
                     AVYRDLARDQFGSSTPLKTFQAVSQVVHEVWSGNVSIGILPFPVGERADPWWRTLAVS
                     GGAAPKIIARLPFVISKRANSNERTALAIAMVEPEPTDDDVSLLAIERLSDISRDRLR
                     AEMKAGNLVAEWLAVWQDPDSPGHALHLVSVEGFLAAADARLTDFRLRAGESVGRLVV
                     LGSYPRPINAAA"
     gene            7944..9056
                     /locus_tag="VGB88_00255"
     CDS             7944..9056
                     /locus_tag="VGB88_00255"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX9645895.1"
                     /translation="MVLGHDRPLKLASGVELGPFTVAYQTYGRLNDERSNVVLVCHAL
                     TGDQYLAGSHPVTGRPGWWDNTVGPGRPLDTDRFHVLCANVLGGCMGSTGPTEIDPAT
                     GKPYGMSFPLITVADMVDAQARLLDHLGIDQVLAVVGGSMGGMQVLDWARRYPERVFS
                     AIPIACSARHTAQNIAFNEVGRRAIMTDPDWHDGNYLLYGTRPSQGLAVARMAAHITY
                     LSDTALHRKFGRGLQDREHVTYGFEADFQVESYLRHQGQSFVERFDANSYLYITRAMD
                     YFDIAAECGGSLQAAFVDTPVRYCLISFTSDWLFPTAESRDIVHALNANAANVSFVEI
                     ETDKGHDSFLLDVPEFNAILSGFLDGVARHRGLDGP"
     gene            9095..9703
                     /gene="metW"
                     /locus_tag="VGB88_00260"
     CDS             9095..9703
                     /gene="metW"
                     /locus_tag="VGB88_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX9645896.1"
                     /translation="MGQRVDLQLIARMIEPGARVLDVGCGDGALLRHLVEHRGVDGRG
                     IEISQAGVNACVRNGLSVVQGDADTDLEDYPDHAFDYVVLSLTLQATHNPRLVLLELV
                     RIGRRAIVSFPNFGYWRCRLQLALGGRMPVTDTLSNPWFATPNIHFCTISDFLELCRE
                     EGIAVERGVALRHDGAAVGFWPGRFLPNMFGEQGVFMLSRRG"
     gene            complement(9687..10436)
                     /locus_tag="VGB88_00265"
     CDS             complement(9687..10436)
                     /locus_tag="VGB88_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9645897.1"
                     /translation="MWQDVSELRQFYASRLGQMSARLIRRRLRGLWPDVSGQRVLGLG
                     YGTPYLRPFLDEAERVIALMPDLQGVSHWPRGEPGLVALCGEESLPLGDNSIDRAVLV
                     HALEASEQLRRLLRELWRVLASGGRMTVVVPNRRGVWARLERTPFGHGHPYSQPQLDH
                     LLRDNMFVPIRSEYALYMPPSRTRMALRLAVAWERVGARWAAPFGGVVITEAEKQVYA
                     GSPLRIARRAPRQVAVPAGTMPAPRRLSRAG"
     gene            complement(10537..11028)
                     /locus_tag="VGB88_00270"
     CDS             complement(10537..11028)
                     /locus_tag="VGB88_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9645898.1"
                     /translation="MATVSMVEYRDAGAEVRALYDEIMATRGIDFVPNVWKTLAVHPP
                     TLRRVWRGLEQVMAPGRLDRLTKEMIAIAVSATNGCEYCVWSHTAAARKLGMDDQMLA
                     EMLAVVGMFNQTNRLANGFQIEVDEAYRQVRAPAKAGPRAKTAAKAARAKAKPGARRA
                     AKR"
     gene            complement(11060..11785)
                     /gene="phbB"
                     /locus_tag="VGB88_00275"
     CDS             complement(11060..11785)
                     /gene="phbB"
                     /locus_tag="VGB88_00275"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX9645899.1"
                     /translation="MTGRVALVTGGTRGIGAAIATALKDAGYRTVATYGGNDERAAKF
                     KDDTGMPVYKWDVSDFAACQSGIAKVAGEVGPVEVLVNNAGITRDGTLHRMSRDMWQE
                     VLDTNLGSCFNMARAVIEPMRQAGFGRIVNISSINGQAGQLGQANYAAAKSGMLGFTK
                     ALAQEGAAKGITVNAIAPGYIDTEMVQAVPAGVLEKIVARIPVGRLGKAEEIARAVLF
                     LVADEAGFITGSTLSINGGQYMS"
     gene            complement(11862..13043)
                     /locus_tag="VGB88_00280"
     CDS             complement(11862..13043)
                     /locus_tag="VGB88_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9645900.1"
                     /translation="MALHLGVASATWLSSLIALPMARAGLLPWAPALAEDGRRLGAEL
                     ATADGGALVAALADAAAARFAAFTAGIEAYRAHPYRRRALRRPVAWRSGTTRLIDHGA
                     GGGVPVVLVPSLVNRAYVLDLAPGRSLAAYLAGRGLRPLVLDWGAPGVAERRFGLDDY
                     VLRRLEPALAYAAAEAGGPVAMVGYCMGGLLALAAALRRPELVAALALLAVPWDFHAD
                     GHAWAQLLPALEPAFAGWIEVAGTFPVEALQTCFAAIQPTQILDKFRAFAALDPDSAE
                     AVEFVQVEDWLNDGVALAGPVARECLFGWYGRNRPARGTWAVGGRPVRPERLSVPSLA
                     FLPARDRIVPPGSAQALARLLPGARLATPRSGHIGMVVGRHAESDCWRPLADWLGQAA
                     I"
     gene            13338..>13817
                     /gene="phaR"
                     /locus_tag="VGB88_00285"
     CDS             13338..>13817
                     /gene="phaR"
                     /locus_tag="VGB88_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX9645901.1"
                     /translation="MARAKSQQADPVVIKKYANRRLYNTSTSSYVTLDNLCDMVKEGT
                     EFVVRDAKSGEDITRSVLTQIIFEQEAKGVNLLPIAFLRRLIGFYDDRLRALVPSYLE
                     VAMETFARNQEQMRQQVQGAFGDLFPVRQMQELSKQNMEMFRQALAMFTPLAGTGGAA
                     "
BASE COUNT         1965 a         5019 c         4879 g         1954 t
ORIGIN      
        1 tctggcgtcg tggccgccgg cttcccttcc ccctggacat taggaacgcg cgggtgtcgg
       61 gtcaagcaaa tgttcatgtt atgttcggta accgccggaa cgtgctggca aggccacctc
      121 aagatggcac agtagcgtct ctggcgcgaa ggggaacccc gatgaggtcg cgatcgtcga
      181 cgaccaccgt tagccgagcg cgggccccgt tgctgcctaa cccgcacccg ggcgagatac
      241 tgctccagga gttcctggtg ccgatgaaac tgagccagaa cgccctcgca cgcgcaatcc
      301 gcgtgccgcc acgccggatc aacgagatcg tgcacgccaa gcgcgcgatc agcgccgaca
      361 cagacctgag gctcgcgcgc tatctcggcg tctcggtagg tttcttcgtc ggcctgcagg
      421 ccgagaaagc ggccttcgca gaggccgaaa tagcgtgcga gcctcgggtc ggtgtcggcg
      481 ctgatcgcgc gcttggcgtg aacgatctcg ttgatccgcc gcggcggcac gcgaacggca
      541 cgcgcgagcg cgttccggct cgatttcatc ggcaccagga actcctggag caggatctcg
      601 cccgggtgcg ggttaggcag caacggggcc cgcgctcagc taacggtggt cgtcgacgat
      661 cacgacctca tcgggtcctc cgtcgcgcca gagacgctac tgtgccatct tgaggtggcc
      721 ttgccagcac gttccggcgg ttaccgaaca taacatgaac atctgcttga cccgacaccc
      781 gcgcgttcct aatgtcgagg gggaagggaa gccggcggcc acgacgcccg ggccggccct
      841 gtgagacgcc catgaccgtc gcgatcgccc cgatctcgcg ccagatctgg gacatgaagt
      901 accgcctgcg cgcgccgagc ggcgagccgc tcgacggcac ggtcgaggac agctggcggc
      961 gcatcgccac cgcgctggcg gtgcccgagg cggaccccgc agcgtggcag ggccggttct
     1021 acgaggcgct cgaggacttc cagttcctgc ctgccgggcg gatcgtcgcc ggggcgggca
     1081 gcaagcgcaa ggtcaccctg ttcaactgct tcgtcatggg cccggtcgcc gacgacatgg
     1141 ccggcatctt cgagtcgctc aaagaggccg cgctgaccat gcagcagggc ggcggcatcg
     1201 gcatcgactt ctcgaccttg cgcccgcgcg gggcggcggt ccacggcgtc ggcgccgacg
     1261 cctcggggcc gatctcgttc atgcgcgtgt gggacgccat gtgccgcacg atcatgagcg
     1321 ccggccaccg gcggggcgcg atgatggcga cgctgtgctg cgaccacccc gacatcgagg
     1381 cgttcatcga cgccaagcgc gagccgggcg agctgcgcat gttcaacctg tcggtgctgg
     1441 tctcggacgc gttcatggac gcgatccggc gcgacgcgcc ctgggacctc gctttcggcg
     1501 gcaccacctg gcggactctt cccgcgcgcg agctgtggca gcggatcatg cgcgcgacct
     1561 acgcctatgc cgagccaggc gtggtgttca tcgaccgcgt caaccgcctc aacaacctct
     1621 cctactgcga ggagatccac gccaccaacc cgtgcgtcac cgccgacacc tgggtgcaga
     1681 cggcggcggg cccggcgcag gtggccgatc tggtcggcag gccgttcacc gccgtcgtcg
     1741 acggccagtc ctgtccgtcc ggcgccgacg gcttcttcgc caccgggcgc aagccggtgc
     1801 tgcggctggt cacggcggcg gggcacacgc tgcggctgac cgcggaccac ccggtgcttc
     1861 gggtcacgcg ccgcacgcgc tggcacgagc agacggactg gtgcccggcc ggcgcgctcc
     1921 gccccgccga ccacatccgc ctgggcaacc agcgaccggc agccgactgg ccgggcgcct
     1981 gtagcgagga ccaagggtac ctgatcgggc tgctgatcgg cgacggcaca ctcaccgccg
     2041 acaaggccgt gctcagcgcc tggcccgggg ctgaggccgt ccacggtggc gtggaacggc
     2101 cgggcgtgct cgcggtcatg gagcgtgcgc tgacggcggc gcggtcgctg ccgcaccgct
     2161 cggacttcgc cggctggatg gcggtgcccg gccgcggcga gcatcgcctg gcgctcggag
     2221 cgatccgggc tcttgccatc gagttagggt tgcgacccgg cgcgaagggt gtgacccccg
     2281 ccctggaacg gagctcggcg gccttccacc gcggcctgct gcgcggcctg ttcgacgccg
     2341 acggcagcgt ccaaggcagc cagagcaagg gcgtcagcgt ccggctcgcg cagagcgacc
     2401 tcgaaatctt gcgggccgtg cagcgcatgc tgctgcgcct cggcatcgcc ggccgcatct
     2461 acgccgagcg gcgccccgcc gggccgcgcc ggctgcccga tgggcgcggt gggacgcagc
     2521 tctacgactg cgccgcagac cacgagctgg tcattgcggg cgacaaccta cagcgcttcg
     2581 ccgagcgcat cggcttcgcc gataccgaca aggcggccgc cctcgggcgc gcgctcgccg
     2641 cctatcgtcg gcggctgaac cgggagcggt tcaccgccgt tgtcgccgcc gtcgagccgg
     2701 acggcgaggc ggaggtctac gacgttcgga ttcccgaccg caacgccttc gacgccaacg
     2761 gcctcatcgt ccataactgc ggcgagcagc cgttgccgcc ctatggggcg tgcctgctgg
     2821 gctcgatcaa ccttgccagc ctggtggggg agccgttcac cgaccaggcc gcgctcgacc
     2881 tcgagcgcct ggaccggctg gtgccggtgg cggtgcgcat gctcgacaac gcgatcgacg
     2941 tctcgcgcta cccgttgccc cggcaggccg aggaggcgcg cgccaagcgc cgcatcgggc
     3001 tcggcatcac gggcctggcc gatgctctga tcatgtgcgg cgcgcgctac ggcagcgcgc
     3061 gcgcggtggc gctgaccgag acctggatgg agcgcttcca gcgcgcggcc tatctcgcct
     3121 ccgccgcgct cgccgccgag aaaggtgcgt tcccgctcta cgacgccgac aagtacctcg
     3181 ccggcgagta tgtcgccggg ctgcccgacg agctgcgcca ggcgatcgcc gccgacggca
     3241 tgcgcaacgg cctggtcacc tcggtcgcgc ccaccggcac catctcgctg atggccgaca
     3301 acgtctcctc agggatagag ccggtctacg ccttcgccca ccggcgcaac gtgctgatgc
     3361 ccgacggcac cacccagtcg accgaggtgg tcgaccacgc ggcccggctc tatcgcgaga
     3421 ccttcggcga gaacgcgccg ctgcccaagt cgttcgtcga cgtccaggcg ctcaccccgg
     3481 ccgaccacct ggtgatggaa gcgaccgtgc agcgctacat cgacagctcg atctcgaaga
     3541 ccatcaactg cccggaggcg atctcgttcg cggacttcga gtcgatctac cagcgcgcct
     3601 acgagctcgg ctgcaagggc tgcaccacct accggccgaa cgtgatcacc ggcgcggtgc
     3661 tcgagaccga ggccgaggcc gcggcgcgcg cggcgccggg cgcgcaagcc gagctcgcgc
     3721 tcgagccgct ggcggtgcgc ccgggcgacg agtacgacgc cggcgcggtg gtctacatga
     3781 cccgcccgct cgaccgcccc gaggagcttc ccggcaccac ctacaagctg cgctggccgg
     3841 agagcgagca cgcgctgtac gtgaccatca acgacatcgt caaggacggc cgccggcggc
     3901 cgttcgagat tttcgtcaac tcgaaaaacc tcgagcatta cgcctggacc atggggttga
     3961 cgcgcatgat cagcgccgtg ttccggcgcg gcggcgacgt gagcttcgtg gtcgacgagc
     4021 tgaaggcggt gttcgacccg cgcggcggcc agtggctggg cggccattac gtgccctcgc
     4081 tcttggccgc gatcggcgag gtggtcgagc gccacatgat cgagatcggc ttcctcaaga
     4141 ccgaggcggc ccgcggcgcc gcaaccggcg ccgccgccgg ggcgttgcgc gcctgcccgc
     4201 gctgcggccg gcgcgcgctg atccgccagg aggactgcga tacctgcacc gcctgcggct
     4261 actcgcactg cgactgagcg gcggcgggct cagccggcgg cggaccgcaa ccgttccgcg
     4321 gccagcacgt ggccgtgcag ccgttcgagc ctgggctcga gacaggcccg ctcgcgcaga
     4381 tggcacacgg tccgttccag gtagtcgcgg cagcggccct tggtgccttc gccttggagc
     4441 accagcgcga ccaggcgcgc gatcggcagc gggccggcgt actggcgatg gccgcgcctg
     4501 acgacgaagg tgtgggcgcg gatgcgccgg ccgggcacgg tcatcgccaa gaatcgcggg
     4561 tggtagacgc tggtccgcat ctcgcgctcg gtcaggtagt cgagcacctc gccggcttgc
     4621 gagcggtgga cccggtaggc ggtgccccgg cacgagccgc cgcggtcgag gccgagcacc
     4681 agcccggggc gctcgggcgt gccgcgatag atgtgcgagt agacgcagaa cgcgcggtga
     4741 tagccgcgca gcagcgccgg ctgcgcgtcg agatagtcga accccggctg ccacatcagc
     4801 gagccgtagg cgaataccca caggtcgttc accgccgcct cgcggctcgc cggcgcgccg
     4861 ccattcggat ataacccgcc cgcaccatgc cgcgcatcct cggactctat gccctgctcg
     4921 ccctcgccgc cctcggctac accgggtact ggttcgtgct cgccgagcgc gccgaggcgc
     4981 tggtcgatgc cgagctcgcc gcctggcggg ccgatggcta cgcggtgtcc tacaccgagc
     5041 gccgcacctc gggctacccc tatcggctgt cgaccgaggt cggcgacttc accctggtgc
     5101 gcgacggcgt gcgctggccg tgggcgtggc acggcgagcg cctggtggtc accctccagc
     5161 cctgggacct cggccattac gtcgccaccc tcgagggcat caaccgcctc gacctgccgc
     5221 cctcgggcga cggcgagagc ttcgcgctca ccgccgcgag cgcgcgcgcg agcctggtcc
     5281 tcgacggccg ctaccaggcc gagcgcggct cggccgagct gcaggacgcg gtgctgcgcg
     5341 aggcgctgtg gcccgacggc gtcaccatcc gccggctgca ggcgcatctc cgccgcccgg
     5401 ccgaggggcc ggacaccaac cccgaggcga gcctcgatgc cgtggtccag gcagacgcgc
     5461 tgcgcctgcc cgacggcgcc ggcggcctgc tcgggcgcga ggtggcgctg gtctatctcg
     5521 agggcacggt gatcggcccg ctgcccggct cgttcgagcc cgagccggtg gcgctgtggc
     5581 gcgattccgg cggcttcatc gatgcccgca gcctggtgct cgaatggggc ccggtgcgga
     5641 tttcggccgc gggctcgctc gccctcgatg cggcgatgcg cccgctcggc gcgctgaccg
     5701 gccggatcgt cggcttcgcc gccctgatcg accggctggc ggcggcgggc cggatgtcgg
     5761 cagaggccgc ggccgcggcc cagctcgcct tcgcgctgat cgccaagacc cccgccggcg
     5821 gcggcgcgcc ggtgctcacc gtgccgctca cgctccagga cggctggctg ttcgccggcc
     5881 cggtgcgcat ggtcccgctc gccccgctgt tcgccgccgc cgacctcgcc ggctgagccc
     5941 cggacctgat ccgggggctc agtcctggcg cgcctcgatc acgccgcggc ggatcgcccg
     6001 ggtctcggtg aagatcttct ccagcacctc gccctcgttg cgccggatcg cgcgttgcag
     6061 gcgcgccagg tcctcgttga aggtcgccag catctcgagc accgcggtgc ggttgttgag
     6121 gaacacgtcg cgccacatca ccgggtcgga ggcggcgatg cgggtgaagt cgcgaaaccc
     6181 gccggcggcg tacttgatca cctcgagctt ggtccggtcc tcgaggtcgg cgaccgtgga
     6241 gacgatgttg taggcgatca ggtgcgggat gtgcgaggtg atcgcgagca cggtgtcgtg
     6301 gtgctccgcg gacatcaggt cgacggtgct gccggccgcg cgccacagcg ccgcgacccg
     6361 ttcgaccgcg cggcggtcgg tatcggcggt gggggtcagc acgcaccagc ggccctcgaa
     6421 cagctcggcg aagccggcat cggggcccga atgctcggtg ccggcgatcg ggtggccggg
     6481 aacgaacgcc acgccctcgg gcagcgccgc gcgcaccgcc tcgatcaccg cgcccttgac
     6541 cgagcccacg tcggtgacga tggcgccggg cgcgagcccg ggcgcgatcg ccgccgcgac
     6601 ctcgccgctg gcgccgaccg gcaccccgat caccaccaga tcggcgcccg cgaccgcctt
     6661 ggccggatcg tcgatcacct ggtcgaccag gccgagctcg gcggcgcgcc ggcgggtggc
     6721 ggcggtgcgc gagtaggcgg ccgtcgcccg cgccaggccc ttggcgcgga tgacgcgcgc
     6781 gagcgagctt ccgatcaggc cgatgccgat cagggcgatg cgctcgaaca gggccgtttc
     6841 ggtcatggcg cgggccgcgc gctcaggcgg cggcgttgat cgggcggggg tagctgccca
     6901 gcaccaccag gcggccgacc gactcgccgg cgcgcaggcg gaagtcggtg agccgcgcgt
     6961 cggcggcggc gaggaacccc tcgaccgaga ccaggtgcaa agcgtggccc ggcgagtcgg
     7021 ggtcctgcca caccgccagc cactcggcga ccaggttgcc cgccttcatc tccgcgcgca
     7081 ggcggtcgcg gctgatgtcg gacaggcgct cgatcgccag caggctgacg tcgtcgtcgg
     7141 tcggttccgg ctcgaccatg gcgatcgcca gcgcggtgcg ctcgttgctg ttggcacgct
     7201 tgctgatgac gaacggtagc ctggcaatta tttttggcgc cgcgccgccg ctgaccgcca
     7261 aggtgcgcca ccacgggtcc gcgcgctcgc cgaccgggaa cggaaggatg ccgatcgaaa
     7321 cattgccgct ccacacctcg tggacgacct ggctgaccgc ctggaaggtc ttgagcgggg
     7381 tcgagctgcc gaactggtcg cgggcgaggt cgcggtacac cgccacccct tcggggacga
     7441 acgcgccgat cgagaagtcc ccctgcaccg atacctgggc ggacaacagc tcgcgccaga
     7501 tgcgcaccag cacctcgacc gggaacgggc ccgaatgccg cgccaccaag cggcgtatga
     7561 tctgcgcctc tcggcccggg cgcatcgcca ccaccgccgg ctcgttctgg cgcttgaccg
     7621 cgccgatccg gccgacgatc tcggtgcgtc gcatcaacag atcgtggatc tggtcgtcga
     7681 tccggtcgat ctcctggcgc agctcatcga tggaccggtc gttcgacatg ccgggattgg
     7741 ggttgggtga gtatcagggg ttgcgcgcct agtagtagcc acggcccccc tcaaaatcaa
     7801 agaaaacatt gacagccggg acgcccgatc cctagctagg ggctcattcg caaacagtgt
     7861 gtttcacggg catggaatcg ggcgaggcga cccggccacg aggcaggttg tattcgggcg
     7921 gcccgatccg gcacgagccg tcggtggtgc tcggccatga ccgcccgctc aagctcgcga
     7981 gcggggtcga gctcggcccg ttcacggttg cctaccagac ctacggcagg ctcaacgacg
     8041 agcgctcgaa cgtggtgctg gtctgccacg cgctgaccgg cgaccagtac ctcgccggca
     8101 gccatccggt gaccgggcgc ccgggctggt gggacaacac ggtcgggccc ggcaggccgc
     8161 tcgacaccga ccgcttccac gtgctgtgcg ccaacgtgct cggcggctgc atgggcagca
     8221 ccgggccgac cgagatcgat cccgcgaccg gcaagcccta cggcatgtcg ttcccgctga
     8281 ttacggtcgc cgacatggtc gacgcccagg cgcgcctgct cgaccatctc ggcatcgacc
     8341 aggtgctggc tgtggtcggc ggctcgatgg gcggcatgca ggtgctcgac tgggcgcggc
     8401 gctatcccga gcgcgtgttc tcggccatcc cgatcgcgtg ctcggcgcgc cataccgccc
     8461 agaacatcgc cttcaacgag gtcggccggc gggcgatcat gaccgatccc gactggcatg
     8521 acggcaacta cctgctgtac ggcacgcggc cgagccaggg cctcgcggtg gcgcgcatgg
     8581 ccgcgcacat cacctatctt tcggacaccg cgctgcaccg caagttcggc cgcgggctgc
     8641 aggaccgcga gcacgtgacc tacggcttcg aggccgactt ccaggtcgag agctacctgc
     8701 gccaccaggg ccagagcttc gtcgagcggt tcgacgccaa ctcctacctc tacatcaccc
     8761 gggcgatgga ctacttcgac atcgccgccg agtgcggcgg ctcgctgcaa gccgcgttcg
     8821 tcgacacccc ggtgcgctac tgcctgatct cgttcaccag cgactggcta ttcccgaccg
     8881 cggagagccg cgacatcgtg catgcgctca acgccaacgc ggccaatgtc agcttcgtcg
     8941 agatcgagac cgacaagggc cacgacagct ttcttctcga cgtgcccgag ttcaacgcca
     9001 tcctgtccgg gttcctcgac ggcgtcgccc gccatcgcgg cctcgacggt ccgtgaacga
     9061 ccagccggca agcaatcgcg actccggccg ggcgatcggc cagcgcgtcg acctgcagct
     9121 gatcgcgcgc atgatcgagc ccggcgcgcg cgtgctcgac gtgggctgcg gcgacggcgc
     9181 gctgctgcgc catctggtcg agcaccgcgg cgtcgacggg cgcggcatcg agatcagcca
     9241 ggccggcgtc aatgcctgcg tcaggaacgg tctgtcggtc gtccagggcg atgccgacac
     9301 cgacctcgag gactatcccg accacgcctt cgactacgtc gtgctcagcc tgaccctgca
     9361 ggcgacgcac aatccgcgcc tggtgctgct ggagctggtg cgcatcggcc ggcgcgccat
     9421 cgtctcgttc ccgaacttcg gctactggcg gtgccggctg cagctcgcgc tcggcgggcg
     9481 gatgccggtc accgacaccc tcagcaaccc gtggttcgcg acccccaaca tccacttctg
     9541 cacgatcagc gacttcctcg agctgtgccg cgaggagggg atcgcggtcg agcgcggggt
     9601 ggcgctgcgc cacgacggcg cggcggtggg cttctggccg ggccgcttcc tgcccaacat
     9661 gttcggcgag cagggcgtgt tcatgctcag ccggcgcggc tgagccggcg cggcgccggc
     9721 atggtgcccg cgggcaccgc cacctggcgc ggcgcgcgcc gggcgatgcg cagcggcgag
     9781 ccggcataga cctgcttctc ggcctcggtg atgaccacgc cgccgaacgg cgctgcccag
     9841 cgcgcgccga cgcgctccca cgccaccgcc aggcgcagcg ccatccgggt tcggctcggc
     9901 ggcatgtaga gggcatattc ggagcggatc ggcacgaaca tgttgtcgcg cagcaggtgg
     9961 tcgagctggg gctggctgta ggggtggccg tggccgaacg gggtgcgctc gaggcgcgcc
    10021 cacaccccgc gccggttggg caccaccacc gtcatccgcc cgccgctggc gagcacgcgc
    10081 cacagctcgc gcagcaggcg gcgcagctgc tcgctggctt cgagggcatg caccagcacg
    10141 gcgcggtcga tgctgttgtc gccgagcggc agcgactcct cgccgcacag cgccaccagc
    10201 ccgggctcgc cgcgcggcca gtggctgacc ccctgcaggt cgggcatcag cgcgatcacc
    10261 cgctcggcct cgtcgaggaa cgggcgcaga tacggggtgc cgtagccgag gccgagcacg
    10321 cgctggcccg agacgtcggg ccacagcccg cgcagccggc gccggatcag gcgcgccgac
    10381 atctgcccca gccgcgaggc gtagaactgg cggagctcgg agacgtcctg ccacatcgcc
    10441 ggtcagacta accgaaacat cccgaagcct gtcccctttc gccggcgcgt tccggcaacc
    10501 attcgccgcc ttacgatcgg cgtggcgcag gtccggtcag cgcttggccg ccctgcgcgc
    10561 gccgggcttg gccttcgccc gcgccgcctt ggccgcggtc ttggcgcgcg gcccggcctt
    10621 ggccggcgcg cggacctggc ggtaggcctc gtcgacctcg atctggaagc cgttggcgag
    10681 ccggttggtc tggttgaaca tgccgaccac ggccagcatc tcggccagca tctggtcgtc
    10741 catgccgagc ttgcgcgccg ccgcggtgtg cgaccagacg cagtactcgc agccgttggt
    10801 ggcgctgacc gcgatcgcga tcatctcctt ggtcagccgg tcgagccggc ccggcgccat
    10861 cacctgctcg agcccgcgcc agacccggcg caaggttggc gggtgcaccg ccagggtctt
    10921 ccagacgttg ggcacgaagt cgatgccgcg cgtggccatg atctcgtcgt agagcgcgcg
    10981 cacctcggcg ccggcgtcgc ggtattcgac catgcttacg gtcgccatgg gctcctccct
    11041 cgctggcttg gccggcggct cagctcatgt actggccgcc gttgatcgac aaggtcgagc
    11101 cggtgatgaa gccggcctcg tcggcgacca ggaacagcac cgcgcgcgcg atctcctcgg
    11161 ccttgccgag ccgcccgacc gggatgcggg cgacgatctt ctcgagcacg ccggccggca
    11221 ccgcctggac catctcggtg tcgatgtagc cgggggcgat ggcattgacc gtgatgccct
    11281 tcgcggcgcc ctcctgggcg agcgccttgg tgaagccgag catgcccgac ttggcggcgg
    11341 cgtagttggc ctggccgagc tggccggcct ggccgttgat cgagctgatg ttgacgatgc
    11401 gcccgaagcc ggcctggcgc atcggctcga tcaccgcccg cgccatgttg aagcacgagc
    11461 cgaggttggt atcgagcacc tcctgccaca tgtcccggct catccggtgc agggtgccgt
    11521 cgcgggtgat gccggcgttg ttgaccagca cctccaccgg gccgacctcg ccggccacct
    11581 tggcgatgcc ggactggcag gcggcgaagt cggagacgtc ccatttgtag accggcatgc
    11641 cagtgtcgtc cttgaacttg gccgcacgct cgtcgttgcc gccataggtc gccaccgtgc
    11701 ggtagccggc gtccttgagc gcggtcgcga tcgcggcgcc gatgccgcgg gtgccgccgg
    11761 tgaccagagc aaccctgccg gtcatgtcct cctcctgagc gtttttatgc actgcaatat
    11821 gagacccgcc tcacagtatg cccgcgcgcc caccacaatg cctagatcgc cgcctgcccc
    11881 agccagtcgg cgagcggccg ccagcagtcg gattcggcgt ggcggccgac gaccatgccg
    11941 atatggcccg agcgcggggt cgcgagccgc gctcccggca gcaggcgcgc cagcgcctgc
    12001 gccgagcccg gcggcacgat ccggtcgcgc gccggcagga aggcgaggct cggcaccgac
    12061 agccgttcgg gccggaccgg ccggccgccg accgcccagg tgccgcgcgc cggccggttg
    12121 cgcccgtacc agccgaacag gcattcgcgc gccaccggcc cggcgagcgc gacgccgtcg
    12181 ttcagccagt cctcgacctg gacgaactcg accgcctcgg cgctgtcggg gtcgagggcg
    12241 gcgaaggcgc ggaacttgtc gaggatctgg gtcggctgga tcgcggcgaa gcaggtctgc
    12301 agcgcctcga ccgggaaggt gcccgcgacc tcgatccagc cggcgaaggc gggctcgagc
    12361 gcgggcaaga gctgcgccca ggcatggccg tcggcgtgga agtcccacgg caccgccagc
    12421 agcgcgagcg ccgccaccag ctccgggcgc cggagcgcgg cagccagcgc cagcagcccg
    12481 cccatgcagt agccgaccat cgccaccggc ccgccggcct cggcggcggc gtaggcgagc
    12541 gcgggctcga gccggcgcag cacgtagtcg tcgaggccga agcggcgctc tgccaccccc
    12601 ggcgcgcccc agtcgagcac cagcgggcgc aggccgcggc cggcgagata tgcggcgagg
    12661 ctgcgtcccg gggcgaggtc gaggacgtag gcgcggttga ccagcgacgg caccagcacc
    12721 accggcacgc cgccgcccgc gccgtggtcg atgaggcgcg tggtgccgga ccgccaggca
    12781 acggggcgcc tcagcgcgcg gcgccggtag ggatgggcgc gataggcctc gatgccggcg
    12841 gtgaaggcgg cgaagcgcgc ggcggcggcg tcggcgagcg ccgccaccag cgcgccgccg
    12901 tccgccgtgg ccagctcagc gccgaggcgg cgtccgtcct ccgccagcgc cggcgcccag
    12961 ggcagcaaac cggcgcgcgc catcggcaag gcgatcagcg aactcagcca ggtcgcgctc
    13021 gccacgccca gatgcagcgc catcggccgc ggcccgagcc gcgccggcgc gggcggcggg
    13081 cgcgaccggc gggcgctcac cggctgcggc ctgcggcgcc tgcgccgcgg cctcgacctg
    13141 gcgctgccac aggtcgagga agcgctcggc gagcgcgcgc gcatgggact cggcggccat
    13201 cgcgggggtg agtatggggc gacgcgtggg cgcgcgcgag cggcgttctt ttcgcactgc
    13261 aacgcatcgg atatagtcct cgccagcctg cgggcggccg ctcgccggcc gcagccagtc
    13321 gaggagcccg cgggcccatg gcgagagcga aatcccagca agccgacccg gtggtgatca
    13381 agaagtacgc caaccgccga ctctacaata cgtccaccag cagctacgtg accctcgaca
    13441 acctgtgcga catggtcaag gagggcaccg agttcgtcgt ccgcgacgcc aagagcggcg
    13501 aggatatcac gcgctcggtg ctgacccaga tcatcttcga gcaggaggcc aagggcgtga
    13561 atttgctgcc gatagccttc ctgcgccggc tgatcggctt ctacgacgac cgcctgcgcg
    13621 cgctggtgcc gtcctatctc gaggtcgcga tggagacctt cgcgcgcaat caggagcaga
    13681 tgcgccagca ggtccagggc gcgttcggcg acctgttccc ggtccgccag atgcaggagc
    13741 tgagcaagca gaacatggag atgttccgcc aggccctggc catgttcacg ccgctcgccg
    13801 gcaccggcgg cgcggcg
//
LOCUS       DASWHU010000009        14223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_372308, whole genome shotgun sequence.
ACCESSION   DASWHU010000009 DASWHU010000000
VERSION     DASWHU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372308"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VGB88_00290"
     CDS             <1..271
                     /locus_tag="VGB88_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9645902.1"
                     /translation="IAADVVARFLAQGYKPEGYTLYTYAAVQVFAAAAEKAGSVDLDA
                     LLPVMRGTTFQTVLGDISFDAKGDVTAPGYVFYEWLDGNYDYANL"
     gene            complement(302..1135)
                     /locus_tag="VGB88_00295"
     CDS             complement(302..1135)
                     /locus_tag="VGB88_00295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9645903.1"
                     /translation="MTAIARAYAALSMAITFAAIAADLQPARADQGFFPIARTPEARF
                     LPVAHSPAAAGQIVPADDSRFFPVRRQAMPEFAATHAPATTILGARPGATAAVIRTDR
                     DFFGLPGHAVARRAPAHPAAVAAAPVDRAPAAGHGPRHAWPVDAAADSRITSRFGWRA
                     DPFTGEPAFHAALDIAAAAGTAAVATAPAVVKAIGAHPRLGRYVMLDFGDGTLATYGH
                     LRDVAVATGDAVARGQRIGGVGSTGRATGPHLDFSFEVAGRRIDPLRVVGPPAALVAG
                     R"
     gene            1399..3099
                     /gene="glgP"
                     /locus_tag="VGB88_00300"
     CDS             1399..3099
                     /gene="glgP"
                     /locus_tag="VGB88_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX9645904.1"
                     /translation="MSPLDPFLGRTHIAYFSMELAIRPEMRSYAGGLGILAGDTARSC
                     ADLELPVVFVTLVSRAGYFRQTIDRDGRQVEAPDWWRPGEWCTPLDAMIAVAIDGRPV
                     WIRPWLYMLSCPHGHRIPILLLDTDLDQNAAADREITHFLYGGDDAYRLKQEIVLGLG
                     GIRLLRALGFDVHTYHLNEGHAALLTLDLLNRWKIAPEDRIAGEPPYDIAEVRERCVF
                     TTHTPVEAGQDRFPYQLFAQLLPDFVDLDALKGLAGADRLNMTRLAFNLSGYVNGVAR
                     RHAETTERMFPGYRIHAVTNGVHVGTWTHESFARLYQAHFPHWQHEPEILVRALQLAE
                     DEVWRCHMEAKHDLISRVKLSAGTDLDPEVPILGFARRMTGYKRPLLLLSDLERLAAI
                     ASRHPFQIVMAGKAHPRDDGGKDSIRQIHDHLRRLTGTVRGAFLADYDMDLAKTLVAG
                     SDVWLNTPLPPLEASGTSGMKAALNGVLNLSVLDGWWIEACVEGINGWAIGADGDGGS
                     DDHHAAALYDKLDGTVLPLFYADPGRWRWMMKQAIGNIAYYFNSQRMMRRYASEAYLR
                     "
     gene            complement(3247..3699)
                     /locus_tag="VGB88_00305"
     CDS             complement(3247..3699)
                     /locus_tag="VGB88_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX9645905.1"
                     /translation="MTAEAENNASEQSLLAMTASVVSAYLSHNALPAAQIPELIHTVF
                     KSLTDTRGPAAALPREPQKPMVSVKRSVTPDYIICLEDGKKLKMLKRHLRTNYNMSPE
                     EYRAKWGLQADYPMVAPNYAKQRSEFAKKIGLGRKRPAAGRRRRRAKA"
     gene            4033..4479
                     /gene="rpiB"
                     /locus_tag="VGB88_00310"
     CDS             4033..4479
                     /gene="rpiB"
                     /locus_tag="VGB88_00310"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX9645906.1"
                     /translation="MADRPESDVIAVAADHAGFPLKQSLAGELQASGYRVLDLGTNNS
                     DSVDYPEYGARMALALLEGRAARGVLICGTGIGVSIAANRHRGIRAAVCHNVETARLA
                     RAHNDANVLVLGGRIVDEVTAKSCLRTFLDTKFEGGRHERRVSMLD"
     gene            4565..5878
                     /gene="glyA"
                     /locus_tag="VGB88_00315"
     CDS             4565..5878
                     /gene="glyA"
                     /locus_tag="VGB88_00315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9645907.1"
                     /translation="MLSQSSPAHHRSAATGGLAASDPELHAAISGELRRQREQIELIA
                     SENIPSEAVLAAQGSVLTNKYAEGYPGRRYYGGCEFVDQAERLAIERCCRLFDCAFAN
                     VQPHSGAQANQAAMLALVKPGDTVLGMSLAAGGHLSHGAKPNLSGKWFHAVQYGVRRD
                     DGLIDYDEVARLAETERPKLIIAGASAYPRRIDFARFRQIADAVGAYLMVDMAHFAGL
                     VAGGAHPSPLPYGHVVTSTTHKTLRGPRGGLIVTTDEDIGKLVNSAVFPGLQGGPLMH
                     VIAAKAVAFGEALQPSFRDYAHAVVANAQALAATLKARGVDIVSGGTDTHLILVDLRP
                     KGLTGKVAEVALERAGLTCNKNAVPFDPEKPTVTSGVRIGTPAGTSRGFGLAEFRRVG
                     ELIGDVIDGLVDKGEAGDREVEQRVRAQVLELCRRFPIYTGLDAG"
     gene            5904..6365
                     /gene="nrdR"
                     /locus_tag="VGB88_00320"
     CDS             5904..6365
                     /gene="nrdR"
                     /locus_tag="VGB88_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9645908.1"
                     /translation="MRCPFCGDEDTQVKDSRPTEDNTVIRRRRFCQSCGSRFTTFERV
                     QLRELTVVKKDGERTPFDREKLARSIEIALRKRPVDAERVERVVSGIVRRLESSGESE
                     IPSSVIGEMVMDALAALDQVAYVRFASVYRNFREARDFEKFVGSLANDQED"
     gene            6373..7506
                     /gene="ribD"
                     /locus_tag="VGB88_00325"
     CDS             6373..7506
                     /gene="ribD"
                     /locus_tag="VGB88_00325"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9645909.1"
                     /translation="MAPERFMRAALALARRGLGNTWPNPAVGCVLVRDEVVVGRGWTQ
                     PGGRPHAETEALRRAGPAARGATAFVTLEPCAHTGATAPCAEALVAAGIARCVVALED
                     PDPRVAGRGLAHLGDAGVEVALGCCADEAAALNAGYLMHRRQGRPMVTWKIASSLDGC
                     IATAAGDSRWITGDAARARAHLLRARHDAVMVGARTAIADQPRLTCRLAGLEAASPVR
                     VIADSRLRLPLTDPLVTEAGAVPSWVLALAGAERGRKRAFTDAGVEVIEVAAGEGGLD
                     MATALAALARRGVTRLMVEGGGRLAASLLRADLVDRIAWFRAPIVLGGDGVPAAAALG
                     LAEIAAGPRFRRTRLLPLDDDVLEDYVRLRDGDHVAPAGESEY"
     gene            7516..8124
                     /locus_tag="VGB88_00330"
     CDS             7516..8124
                     /locus_tag="VGB88_00330"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9645910.1"
                     /translation="MFTGIVTDVGRVHEVVRGGEVRLAIDTRYDTTRIAPGASIACAG
                     VCLTVVEAGAGRFAVEVSAETLARTTLGGWRVGTRVNLERALSVGDELGGHLVSGHAD
                     GTAEVLARTPIGDSLVVWLALPDVLAPYVAAKGSVALDGVSLTVNDTRPDRFAVNLIP
                     YTRANTTLGDLAAGDRVNVEADMLARYVRRALAYAGRETEPA"
     gene            8124..9257
                     /gene="ribB"
                     /locus_tag="VGB88_00335"
     CDS             8124..9257
                     /gene="ribB"
                     /locus_tag="VGB88_00335"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX9645911.1"
                     /translation="MAKAADNISDARSVFASIADIIADARAGRIFVLVDDERRENEGD
                     LVIPAATATAEAVNFMARYGRGLICLALSAERAAQLKLEPMQRRNAARFDTAFTVSIE
                     AKEGISTGISAADRARTIATAIDERAGPEDLATPGHVFPVVARDGGVLVRAGHTEAAV
                     DIARLAGLPPAGVICEIMNDDGTMARRNDLIGYCRRHGLRIGTISELIAYRRRHDRLV
                     ERRLERSFDSHYGGTFRMVVYANTLDGTEHVALVKGDVAGGGPVLVRVHKLDVVADLL
                     GEAADGRGRELHAAMELVGREGRGVIVLIGEDRPRALSDRLSSTDTGAGSELRDVGLG
                     SQILLDLGVRAMCVLSNSERTIVGLDGFGLEVAGWRPIPTGKG"
     gene            9259..9702
                     /locus_tag="VGB88_00340"
     CDS             9259..9702
                     /locus_tag="VGB88_00340"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9645912.1"
                     /translation="MAAAGPRVLVVVARFYDDLADALVAGARAALEAAGAEIEIVEVP
                     GAFEVPGAIAFAHRSGRFDGFVALGCVIRGETSHYDLICRESARGLGDLAVHHGAALG
                     YGILTTDTREQARVRAAVDGGNKGGDAAHACLRMIEIRNRFVTQS"
     gene            9699..10217
                     /gene="nusB"
                     /locus_tag="VGB88_00345"
     CDS             9699..10217
                     /gene="nusB"
                     /locus_tag="VGB88_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9645913.1"
                     /translation="MSAPEILPEASAREHRRKARSLRRRARVAAVQALYQVELVGAAP
                     AAVIGEFADRLAGGPAMDFEFFRTLVTGACAARELIDQRISANLAKGWRIGRLSIPMR
                     ALLRAAAFELDRCRDIPPKVTVSEYVDIAREFFDRSETGFVNGVVETLARSLRADDWP
                     PSERLRDGGTPG"
     gene            10198..11184
                     /gene="thiL"
                     /locus_tag="VGB88_00350"
     CDS             10198..11184
                     /gene="thiL"
                     /locus_tag="VGB88_00350"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9645914.1"
                     /translation="MAERPGEFELIERLFRPLADGFEGAFGLTDDAAVIPLGAGRVAV
                     ATQDALVAGVHFPAGEAPEAVAAKALRVNLSDLAAMGAEPVAYLLALALPAEVDLAWL
                     ERFADALGRDQRRYAIALAGGDTVATPGPLMIAITAIGAAEPGRVLRRNGARAGDAVF
                     VSGTIGDAALGLDSLAGRLPAAVPAEVREALVARLRYPEPRLALGRALVGVASAAIDV
                     SDGLAADLGHVAAASAVAAELRAASVPLSAAARAVLAAAPALITRVLAGGDDYELLFT
                     APAKARDAVLRAAAAAGVPVTEIGEIRAGSGVRAVAADGTEVPLGAGGWTHF"
     gene            11322..13355
                     /locus_tag="VGB88_00355"
     CDS             11322..13355
                     /locus_tag="VGB88_00355"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9645915.1"
                     /translation="MSLALVFALACGAAAIAYGIFTTTRVMAAATGNARMQEIAAAIQ
                     EGARAYLNRQYSAIAVAGIVIGAVLWWILGLHVGIGYLIGAVLSGAAGYIGMNISVRA
                     NVRTAEAARQGLDPALTLAFRAGAITGMLVVGLGLLGVAGYFAVLLGVYGDQAPANMR
                     HILEALVALSFGASLISIFARLGGGIFTTGADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTIIATMLLGSLVLPSFGNAVMYPLVLGGVSILASIL
                     GTFTVKVSKKEKIMNGLYRAVITSGVLAAIAFWFVTDKMMAGNGSYSTMQIYGAALIG
                     LALTTAMVVITEYYTATQYSPVRGIAQASTTGHGTNVIAGLGLSMKATALPVLAVCAS
                     IWGAYELAELYGIAIAATSMLSMTGVIVALDAYGPVTDNAGGIAEMAELPKKVRDVTD
                     PLDAVGNTTKAVTKGYAIGSAGLAALVLFADFTHALEAHVGQAVEFSLSNPNVIIGLF
                     IGGLIPYLFGALAMEAVGRAAGSVVVEVRRQFKTIKGIMEGKAKPDYSRAVDMLTKAA
                     IKEMIIPSILPVLVPIVVGFTLGKEALGGVLLGSIVTGIFVAISMTTGGGAWDNAKKY
                     IEDGKHGGKGSDAHKAAVTGDTVGDPYKDTAGPAINPMIKIINIVALLIVPLL"
     gene            13557..>14223
                     /locus_tag="VGB88_00360"
     CDS             13557..>14223
                     /locus_tag="VGB88_00360"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEX9645916.1"
                     /translation="MVDTSRRPPLFTPHRRALTVLYADLESHALGQHEVFVGTAGSVI
                     ERKNASGFRFYAHQFYDGEGRKRERYLAGPVGDPKADAAAADLRERIKELKEIVPSLR
                     LLGREGFNLVDARTYATLASLHNHGVFGAGGMLIGSHAYGVLLNRLGVRATPYATEDI
                     DVARREALAFEKQPEQGLLDMLKDSGISFVEVPSLNRKKPPTSFKQRGRSRFHVDLLV
                     PSAN"
BASE COUNT         2076 a         4996 c         4939 g         2212 t
ORIGIN      
        1 gatcgcggcc gacgtggtcg cgcgcttcct ggcccagggc tacaagcccg agggctacac
       61 gctctacacc tatgccgcgg tccaggtgtt cgccgcggcg gccgagaagg cgggcagcgt
      121 cgacctcgac gccctgctgc cagtgatgcg cggcaccacc ttccagaccg tgctcggcga
      181 tatcagcttc gatgccaagg gcgacgtgac cgcgccgggc tatgtgttct acgagtggct
      241 ggacggcaac tacgactacg ccaacctgtg atccgccgcc gccgcggcgc gtaagagggg
      301 cctaacggcc ggcgaccagc gccgccggcg ggccgacgac cctgagcggg tcgatgcgtc
      361 ggcccgccac ctcgaacgag aagtcgaggt gcgggccggt ggcgcgcccg gtgctgccga
      421 cgccgccgat gcgctggccg cgcgcgaccg catcgccggt cgcgaccgcg acgtcgcgga
      481 ggtggccgta ggtcgccaac gtcccgtcgc cgaagtccag catgacgtag cggccgaggc
      541 gcgggtgcgc gccgatcgcc ttgaccaccg ccggggcggt ggcgaccgcc gcggtgccgg
      601 cggccgcggc gatgtcgagg gcggcgtgga aggcaggctc gccggtgaac ggatcggccc
      661 gccagccgaa gcgcgaggtg atccggctgt ccgccgccgc atccaccggc caggcatgtc
      721 gcgggccgtg gccggcggcg ggcgcgcgat cgactggggc tgcggcgacc gcggcggggt
      781 gcgccggggc acggcgggcg acggcgtggc cgggcaggcc gaaaaagtcg cggtcggtgc
      841 gaatgaccgc ggccgtcgcg ccgggacggg cgccgaggat ggtcgtggca ggggcatggg
      901 tggcggcgaa ctcgggcatc gcctggcggc gaaccgggaa gaagcggcta tcgtcggccg
      961 gcacaatttg cccggccgcg gccggggaat gcgcgaccgg caggaagcgc gcctcggggg
     1021 tgcgcgcgat cggaaagaag ccctggtccg cgcgcgcagg ttgcaggtcc gcggcaatgg
     1081 cggcgaacgt aatcgccatc gacagggcgg cgtaggctcg cgcgattgcc gtcacggccg
     1141 gctccgcaac gcggtttcgg ttcggtcaag gtaccggaag caactcccgt gccgacttca
     1201 ccgcgggccg aaaagacgcc gcgcgaacgc gcggtcggac gacattccgg caatgattcg
     1261 gggtgcccga tcacgggttt gtaacggacg ccctcaacga tgaccgatgc ctgcccgggc
     1321 cttctgccgt aaccgaccga ttcgccgccg gcaaaggcct cgattgacgc gcgatggcat
     1381 ttgagcggag cttccgccat gtcgcctctc gaccccttcc tcggccgcac gcacatcgcc
     1441 tacttctcga tggagctggc cattcggccc gagatgcgca gctacgccgg cgggctcggc
     1501 attctcgccg gcgacaccgc gcgctcctgc gccgacctgg agctgcctgt ggtgttcgtc
     1561 accctggtca gccgggccgg gtatttccgc cagacgatcg atcgggacgg ccggcaggtc
     1621 gaggcccccg actggtggcg gccgggcgaa tggtgcacgc cgctggacgc catgatcgcg
     1681 gtggcgatcg acggccggcc ggtgtggatc cggccctggc tctatatgct gagctgcccg
     1741 cacgggcatc gcatcccgat cctgctgctc gataccgacc tcgaccagaa tgccgctgcc
     1801 gaccgcgaga tcacccactt cctgtatggc ggcgacgacg cctatcgcct caagcaggag
     1861 atcgtgctcg ggctcggcgg catcaggctg ttgcgggcgc tcggtttcga cgtgcacacc
     1921 taccacctca acgagggcca tgcggcgctg ctgactctcg acctgctgaa ccgctggaag
     1981 atcgcgccgg aggaccgcat cgccggcgag ccgccgtacg acatcgccga ggtgcgcgag
     2041 cgctgcgtgt tcaccaccca caccccggtc gaggcgggac aggatcgctt tccctaccag
     2101 ctgttcgcgc agctgctgcc cgatttcgtc gacctcgatg cgctcaaggg cctcgccggc
     2161 gccgaccggc tcaacatgac gcggctcgcc ttcaacctct cgggctacgt caacggcgtg
     2221 gcgcgccgcc atgccgagac gaccgagcgc atgttcccag gctaccggat ccatgccgtc
     2281 accaacggcg tgcatgtcgg cacctggacg cacgagagct tcgcccgcct ctatcaggcc
     2341 cacttcccgc attggcagca tgagccggag atcctggtgc gcgccctgca gctggcggag
     2401 gacgaggttt ggcgctgcca catggaggcc aagcacgacc tgatcagccg ggtcaagttg
     2461 tccgccggca cggacctcga tcccgaggtg ccgatcctgg gcttcgcgcg ccgcatgacc
     2521 ggctacaagc gcccgctgtt gctgctcagc gaccttgagc ggctggccgc gatcgcatcg
     2581 cgccatccgt ttcagatcgt gatggcgggc aaggcgcacc cgcgtgacga cggcggcaag
     2641 gactcgatcc gccagattca cgatcacctg cggcgtctta cgggcacggt ccgcggcgcc
     2701 tttctggcgg attacgacat ggatctcgcc aagaccctgg tcgccggatc ggacgtctgg
     2761 ctgaacacgc cgctgccccc gctcgaggcg tcgggcacca gcggcatgaa ggcggcgctc
     2821 aacggcgtcc tgaacctgag cgtgctcgac ggctggtgga tcgaagcctg cgtcgagggg
     2881 atcaacggct gggcgatcgg cgcggacggc gacggcggct cggatgacca ccacgccgcc
     2941 gcgctctatg acaagctgga cggcaccgtg ctaccgctct tttacgccga tccggggcgg
     3001 tggcgctgga tgatgaagca ggcgatcggc aacatcgcct actacttcaa cagccagcgc
     3061 atgatgcgcc gctacgcgag cgaggcgtat ctgcgctgag gcttcaacgg ccccgccctc
     3121 aggccagcct caaagccggc cgcgcaatcc ccgactaagc gccattgccg tcggcgctgg
     3181 tcagcgggga aagcagtaac cggggctcgg aagctctttg cgagtcgacc cgcgaggcgg
     3241 tcggtattac gccttcgccc ggcgccggcg ccggccggcc gccggccgct tgcggccgag
     3301 gccgatcttc ttggcgaact ccgagcgctg cttggcgtaa ttgggcgcga ccatcggata
     3361 gtcggcttgc agtccccact tggcccggta ctcctcgggc gacatattgt agttggtgcg
     3421 cagatgccgc ttgagcatct tgagtttctt gccgtcttcc agacagatga tgtaatcggg
     3481 cgtcaccgac cgcttgaccg agaccattgg cttttgcggc tccctgggca gggcggcggc
     3541 aggtccgcgc gtgtcggtca gcgacttgaa tacggtatga atcagttccg gtatttgggc
     3601 cgcaggtagc gcgttgtggc tcaaataggc ggacacaacg ctcgccgtca tcgcgagcaa
     3661 ggattgttca ctggcattat tttcagcttc tgcggtcatc tttgtacgcc tgattaaagt
     3721 tgcggatcgc gacactgact gacagggctc acacagaatg tcaacgggta aattcgaatt
     3781 tcggcgcgac gagttttgtc gccgaccgtg ggcaatgctc ttaaccagta tccggattac
     3841 tgttcctgcg gtaagcgatg ccttggaact agagattcgc tggcgcgccg atgctcgttt
     3901 tgcgtgtaca tggccgggct gatcccgttc gggcgggccg ccggcatgtc ctggcacgcg
     3961 tcaccaacat gtggtaatca gcggcccagt tcacacaact tgtcgagctt ggcagggatc
     4021 gcgaggcagg ccatggcgga ccggcccgaa tcggatgtta ttgcggtggc cgcggaccat
     4081 gcggggtttc cgctcaagca gtcgctggcg ggcgagctgc aagcgtccgg ctatcgggtt
     4141 ctcgacctcg gcaccaacaa ctccgactcg gtcgactacc cggagtacgg cgccaggatg
     4201 gcgctcgcgc tgcttgaggg ccgggcggcg cgcggggtgc tgatctgcgg caccggcatc
     4261 ggggtcagca tcgccgccaa ccgccaccgg ggaatcaggg ccgcggtctg ccacaatgtc
     4321 gagaccgcgc ggctcgcgcg ggcgcacaac gacgccaacg tcttggtgct cggcgggcgg
     4381 atcgtcgacg aagttaccgc caagtcttgc ctgcgcacgt tcctcgacac caagttcgaa
     4441 ggtggccggc acgaacgccg tgtctccatg ctcgattgat ccggggcgtt tctcagctat
     4501 ttgttgcacg gatgccctta ttttcaaccg tctacgacta accaggccga cgggaccgat
     4561 attgatgctg tcacagtctt cccccgctca ccaccgcagc gccgcgaccg gcggcttggc
     4621 cgccagcgat cccgagctcc atgccgccat ttccggggag ctcaggcgcc agcgcgagca
     4681 gatcgagctg atcgcctcgg agaacatccc cagcgaggcg gtgctcgccg cccagggctc
     4741 ggtgctgacc aacaagtatg ccgagggcta tccagggcgg cgctactacg gcggctgcga
     4801 gttcgtcgac caggccgagc ggctggcgat cgagcgctgc tgccggctgt tcgactgcgc
     4861 cttcgccaac gtccagcccc actccggagc gcaggccaac caggcggcga tgctggcgct
     4921 ggtcaagccg ggcgacaccg tcctcggcat gtcgctcgcc gcgggcggcc atctcagcca
     4981 cggcgccaag cccaacctgt ccggcaagtg gttccacgcc gtccagtacg gggtgcgccg
     5041 cgacgacggc ctgatcgact atgacgaggt cgcccgcctc gccgagaccg agcgtcccaa
     5101 gctgatcatc gccggtgcct cggcctatcc gcgccggatc gacttcgccc gcttccgcca
     5161 gatcgccgac gcggtcggcg cctacctgat ggtcgacatg gcccatttcg ccggcctggt
     5221 cgcgggcggc gcgcatccga gcccgctgcc atacggccac gtcgtcacca gcaccaccca
     5281 caagacgctt cgcggcccgc gcggcgggct gatcgtcacc accgacgagg acatcggcaa
     5341 gctggtcaat tcggcggtgt tcccgggcct gcaaggcggc ccgctgatgc acgtgatcgc
     5401 ggccaaggcg gtggcgttcg gcgaggctct ccagccgtcg ttccgcgact atgcgcacgc
     5461 cgtggtcgcc aacgcccagg cgctcgcggc caccctcaag gcgcgcggcg tcgacatcgt
     5521 cagcggcggc accgacaccc atctgatcct ggtcgacctg cggccgaagg gcctgaccgg
     5581 caaggtcgcc gaggtggcgc tcgagcgcgc cgggctcacc tgcaacaaga acgcggtgcc
     5641 gttcgatccc gagaagccga ccgtcacctc gggcgtgcgc atcggcacgc cggccggcac
     5701 cagccgcggc ttcggcttgg ccgagttccg ccgcgtcggc gagctgatcg gcgacgtcat
     5761 cgacggcctg gtcgacaagg gcgaggccgg cgaccgcgag gtcgagcagc gcgtccgcgc
     5821 ccaggtgctc gagctgtgcc ggcggtttcc catctacacc gggctcgacg ccggctgagg
     5881 gcggcgcgca cggggagtaa gcgatgcgct gtccgttctg cggcgatgaa gacacccagg
     5941 tcaaggactc gcgcccgacc gaggacaaca cggtcattcg ccggcgccgc ttctgccagt
     6001 cctgcggctc gcgtttcacc accttcgagc gcgtccagct acgcgagctg acggtggtca
     6061 agaaggacgg cgagcgcacc ccgttcgacc gcgagaagct cgcgcgctcg atcgagatcg
     6121 cgctgcgcaa gcgcccggtc gacgccgagc gggtcgagcg ggtggtcagc ggtatcgtcc
     6181 gccgcctcga gagctcgggc gagtccgaga tcccgtcgtc ggtgatcggc gagatggtaa
     6241 tggacgcgct cgccgcgctc gaccaggtcg cctatgtccg cttcgcctcg gtctaccgca
     6301 acttccgcga ggcgcgcgac ttcgagaagt tcgtcggcag cctggccaac gaccaggagg
     6361 actgagtgcg ccatggcgcc cgagcgcttc atgcgcgcgg cgctggcgct cgcccggagg
     6421 gggctcggta acacctggcc caatcccgcg gtcggctgcg tgctggtgcg ggacgaggta
     6481 gtggtcgggc gcggctggac ccagccgggc gggcgcccgc acgccgagac cgaggccctg
     6541 cgccgtgccg gtcccgcggc ccgcggcgcc accgccttcg tcaccctcga gccgtgcgcg
     6601 cataccggcg ccaccgcgcc gtgcgccgag gcgcttgtcg cggccggcat cgcccgctgc
     6661 gtggtggcac tcgaggaccc cgacccacgc gtcgccggcc gaggcctcgc ccatctcggt
     6721 gacgccggcg tcgaggtcgc gctcggctgc tgcgcggatg aggccgccgc gctcaacgcc
     6781 ggctacctca tgcaccgccg ccaggggcgg ccaatggtga cctggaaaat cgcgagcagc
     6841 ctcgacggct gcatcgccac cgccgccggc gacagccgct ggatcaccgg cgacgcggcg
     6901 cgcgcgcgtg cgcacctgct gcgcgcccgc cacgatgcgg tgatggtcgg ggcgcggacc
     6961 gcgatcgccg accagccccg gctcacctgc cgcctggcgg ggctggaagc ggcctcgccg
     7021 gtgcgcgtga tcgccgacag ccgcctgcgc ctgccgctga ccgacccgct ggtcaccgag
     7081 gcgggcgccg tgccgagctg ggtgctggcg ctcgccggcg ccgagcgcgg gcgcaagcgg
     7141 gcgttcaccg atgccggcgt cgaggtaatc gaggtcgcgg cgggggaagg ggggctcgac
     7201 atggcaacgg cactcgccgc gttggccagg cgcggcgtca cccggctgat ggtcgaaggc
     7261 ggcggccggc ttgccgccag cctgctgcgc gccgacctgg tcgaccgcat cgcctggttc
     7321 cgggcgccga tcgtgctcgg cggcgacggc gtgcccgccg cggcggcgct ggggctcgcg
     7381 gagatcgcgg ccgggccgcg gtttcggcgc acgcggctgc tgccgctcga cgacgacgtg
     7441 ctcgaggact acgtgcgcct gcgcgacggc gatcacgttg cgccggccgg ggaaagtgag
     7501 tactagctgt cgcacatgtt caccggcatc gtcaccgatg tcggacgggt ccatgaggtc
     7561 gtgcgcggcg gcgaggtgcg cctggccatc gacacccgtt acgacaccac caggatcgcg
     7621 ccgggcgcct cgatcgcctg cgccggcgtc tgcctgaccg tggtcgaggc cggcgccggc
     7681 cggttcgcgg tcgaggtctc ggcggagacc ctggcgcgga ccacgctcgg cggctggcgg
     7741 gtcggcaccc gggtcaacct cgagcgcgcg ctcagcgtcg gcgacgagct tggcgggcat
     7801 ctggtcagcg gccatgccga cggcaccgcc gaggtgctcg cgcgcacccc catcggcgac
     7861 tcgctggtgg tctggctcgc gctcccggac gtccttgcgc cctacgtcgc ggccaaggga
     7921 tcggtggcgc tggacggagt ctcgttgacc gtcaacgaca cccgccccga ccggttcgcg
     7981 gtcaacctga tcccctacac ccgggccaac accaccttgg gcgacctcgc cgccggcgac
     8041 cgggtcaatg tcgaggccga catgctcgcg cgctatgttc gccgggcgct cgcttatgcg
     8101 ggcagggaga cggagccggc gtgatggcca aggcggcgga caacatctcc gacgcacgct
     8161 cggtgttcgc ctcgatcgcc gacatcatcg ccgacgcccg cgccggccgc atattcgtcc
     8221 tggtcgatga cgagcgccgc gagaacgagg gcgacctggt gatcccggcg gccacggcga
     8281 ccgcggaggc ggtcaacttc atggcccgct acggtcgcgg cctgatctgc ctggcgctgt
     8341 ccgccgagcg cgccgcgcag ctcaagctcg agccgatgca gcggcgcaac gccgcccggt
     8401 tcgataccgc gttcacggtc tcgatcgagg ccaaggaggg catctccaca ggcatatcgg
     8461 cagccgaccg cgcgcgcacc atcgccaccg ccatcgacga gcgcgccggg cctgaggacc
     8521 tggcgacacc cgggcacgtg ttccccgtgg tcgcgcgcga cggcggcgtg ctggtccgtg
     8581 ccggccatac cgaggccgcg gtcgatatcg cgcgcctcgc cggcctgccc ccggccgggg
     8641 tgatctgcga gatcatgaac gacgacggca ccatggcccg gcgcaacgac ctgatcggct
     8701 attgccgccg ccacggcctg cgcatcggca ccatctccga gctcatcgcc tatcgccggc
     8761 gccacgaccg gctggtcgag cggcggctcg agcgcagctt cgacagccac tacggcggca
     8821 ccttccggat ggtggtctac gccaacaccc tcgacggcac cgagcacgtg gcgctggtca
     8881 agggcgacgt cgccggcggc gggccggtgc tggtgcgggt ccacaagctc gacgtggtcg
     8941 ccgacctgct gggcgaggcg gccgacgggc gcgggcgcga gctgcacgcg gcgatggagc
     9001 tggtcgggcg cgagggccgc ggcgtgatcg tgctgatcgg cgaggaccgg ccgcgggcgc
     9061 tcagcgaccg gctgtcgagc accgacaccg gcgccggcag cgagctccgg gacgtcggcc
     9121 tcggctcgca gatcctgctc gacctcgggg tgcgcgcgat gtgcgtgctc agcaactccg
     9181 agcgcaccat cgtcggcctc gacggcttcg gcctcgaggt cgccggctgg cggccgatcc
     9241 cgaccgggaa gggctgaggt ggccgcggcc gggccgcgcg tcctggtcgt ggtcgccagg
     9301 ttctacgacg acctcgcgga tgcgcttgtc gccggcgcgc gggcggcgct cgaggcggcc
     9361 ggcgccgaga tcgagatcgt cgaggttccg ggcgcgttcg aggtcccggg cgcgatcgcc
     9421 ttcgcccacc ggagcggccg cttcgacggc ttcgtcgcgc tcggttgcgt gatccgcggc
     9481 gaaacctcgc attatgacct catctgccgc gagagcgcgc gcgggcttgg cgacctcgcg
     9541 gtccatcacg gcgccgcgct cggatatggc atattgacca ccgatacgcg cgagcaggcg
     9601 cgggtgcggg cggcggtcga cggcggcaac aagggcggag atgccgcgca cgcctgcctg
     9661 cgcatgatcg agatcaggaa tcggttcgta acgcaatcat gagtgcacct gagattttgc
     9721 ctgaggccag cgcccgcgag caccgccgca aggcgcgctc gctgcgccgg cgcgcgcgcg
     9781 tcgcggcggt ccaggcgctc tatcaggtcg agctggtggg cgcggccccg gcggctgtca
     9841 tcggcgagtt cgccgaccgg ctggcgggcg gcccggcgat ggatttcgag ttcttccgga
     9901 cgctggtcac cggcgcctgc gccgcacgcg agctgatcga tcagcggatt tcggccaacc
     9961 tggccaaggg ctggcggatc gggcggctat cgatcccgat gcgcgcgctg ttgcgcgcgg
    10021 ccgcgttcga gctcgaccgc tgccgcgaca tcccgcccaa ggtgaccgtg agcgagtatg
    10081 tcgacatcgc gcgcgagttc ttcgaccggt ccgagaccgg cttcgtcaac ggcgtggtcg
    10141 agaccctggc ccgcagcctg cgggccgacg actggccgcc aagcgagcgg ctgcgcgatg
    10201 gcggaacgcc cgggtgagtt cgagctgatc gagcgcctgt tccgcccgtt ggcggacggc
    10261 ttcgagggcg ccttcggtct cacggacgac gccgcggtga tcccgctcgg cgccggacgg
    10321 gtcgcggtcg cgacccagga cgcgctggtc gcgggcgtcc acttcccggc cggcgaggcg
    10381 cccgaggcgg tcgccgccaa ggcgctcagg gtgaacctga gcgacctcgc cgcgatgggc
    10441 gccgagccgg tcgcctatct gctcgcgctc gccttgcccg cggaggtcga tctcgcctgg
    10501 ctcgagcggt tcgcggacgc gcttgggcgc gaccagcgcc gctacgcgat cgccctcgcc
    10561 ggcggcgaca cggtcgcgac cccggggccg ctgatgatcg cgatcaccgc catcggcgcc
    10621 gccgagcccg gccgcgtgct gcggcgcaac ggcgcgcgcg ccggcgatgc cgtgttcgtc
    10681 tcgggcacca tcggcgatgc cgcgctcggg ctcgattcgc tcgccggccg cctgcccgca
    10741 gccgtgccgg cggaagttcg cgaggcattg gtcgcgcggc tgcgctatcc ggagccgcgc
    10801 ctcgccttgg gacgggcgct ggtcggcgtg gcctccgccg cgatcgacgt ctccgacggc
    10861 ctcgccgccg atctcggcca tgtcgccgcc gcatcggcgg tggcggccga gctgcgcgcg
    10921 gcgtcggtgc cgctgtcggc ggcggcgcgc gcggtactcg ccgcggcgcc cgcgctcatc
    10981 acccgcgtgc tcgccggcgg cgacgactac gagctcttgt tcaccgcgcc agcgaaggcg
    11041 cgcgacgcag tcttgcgcgc cgccgctgcg gccggcgtgc cggtcactga gatcggcgaa
    11101 atacgcgccg gcagcggcgt gcgcgcggtc gccgccgacg gcaccgaggt ccctctcggg
    11161 gccggcggct ggacccattt ctaggcccgg cccgccggcg ccgggcgctg cccgcgctga
    11221 ttgcattctc gcccgcgctc tggcattttt ccgcgcgcat cggcgccaat gctcggggac
    11281 aggcattggc gcatttgatt ggagcaaggg gaatcgcata gatgtcgctt gcattggtgt
    11341 tcgcgctcgc ctgcggcgcc gccgccatcg cctacgggat cttcaccacc acccgggtga
    11401 tggccgccgc caccggcaac gcgcgcatgc aggagatcgc cgccgcgatc caggagggcg
    11461 cgcgcgccta cctcaaccgc cagtacagcg ccatcgcggt cgccggcatc gtgatcgggg
    11521 ccgtgctgtg gtggatcctc ggcctgcatg tcggcatcgg ctacctgatc ggcgcggtgc
    11581 tgtcgggcgc cgcaggctat atcggcatga acatctcggt gcgcgccaac gtgcgcaccg
    11641 ccgaggccgc ccgccagggg ctcgatccgg cgctgaccct cgccttccgc gcgggcgcca
    11701 tcaccggcat gttggtggtc ggtctcggcc tgctcggggt cgccggctat ttcgcggtct
    11761 tgctcggcgt ctacggcgac caggcgcccg cgaacatgcg ccacatcctg gaagcgctgg
    11821 tcgcgctcag cttcggcgcc tcgctgatct cgatcttcgc ccggctcggc ggcggcatct
    11881 tcaccacggg cgccgacgtc ggcgccgacc tggtcggcaa ggtcgaggcc ggcattcccg
    11941 aggatgaccc gcgcaacccg gcggtgatcg ccgacaacgt cggtgacaac gtcggcgact
    12001 gcgccggcat ggccgccgac ctgttcgaga cctacgccgt gaccatcatc gcgaccatgc
    12061 tgctcggcag cctggtgctg ccgtccttcg gcaacgccgt gatgtatccg ctggtgctcg
    12121 gcggcgtgtc catcctcgcc tccatcctcg gcaccttcac cgtcaaggtc agcaagaagg
    12181 aaaaaatcat gaacggcctg taccgcgccg ttatcacctc gggcgtgctg gccgccatcg
    12241 ccttctggtt cgtgacggac aagatgatgg ccggcaacgg ctcctacagc accatgcaga
    12301 tttacggcgc ggcgctcatc gggctggcgc tcaccaccgc catggtggtc atcaccgagt
    12361 actacaccgc gacccagtac agcccggtgc gcgggatcgc ccaggcctcg accacgggcc
    12421 atggcaccaa cgtcatcgcg gggctcggcc tgtccatgaa ggccacggcg ctgccggtgc
    12481 ttgcggtgtg cgccagcatc tggggcgcct atgagctcgc cgagctctac ggcatcgcca
    12541 tcgcggccac ctcgatgctc tcgatgaccg gcgtcatcgt ggcgctcgat gcctacggcc
    12601 cggtcaccga caacgccggt ggtatcgccg aaatggcgga actgccgaaa aaggtgcgcg
    12661 acgtcaccga tccgctcgac gccgtgggca acaccaccaa ggccgtcacc aagggctacg
    12721 ccatcggctc ggccggtctc gcggcgctgg tgctgttcgc ggatttcacg cacgcgctcg
    12781 aagcgcacgt gggtcaggcg gtggaattca gcctgtccaa tcccaacgtg atcatcggcc
    12841 tgttcatcgg tggcctcatc ccctacctct tcggcgccct cgccatggag gcggtcggcc
    12901 gcgccgccgg cagcgtggtg gtggaagtac gccgccagtt caagaccatc aagggcatca
    12961 tggaaggcaa ggccaagccg gattattcca gggccgtgga catgctgacc aaggcggcga
    13021 tcaaggaaat gatcatcccc tcgatactgc cggtgctggt gccgatcgtg gtcggcttca
    13081 cgctcggcaa ggaggcgctg ggcggcgtgc tgctcggctc catcgtcacc ggcattttcg
    13141 tcgccatctc catgaccacc ggcggcggcg cctgggacaa cgccaagaaa tacatcgagg
    13201 acggcaagca cggcggcaag ggctcggacg cgcacaaggc cgccgtcacc ggcgacaccg
    13261 tgggcgaccc ctacaaggac acggccggcc cggcgatcaa ccccatgatc aagatcatca
    13321 atatcgtggc gctgttaatc gtccctctct tataagagat tttcaccaac tccccctctc
    13381 ccgcttgcgg gagagggcaa ggggagaggg ttacatcgtc aagaagggct ggctttgccg
    13441 gcccttttgt tttatccacc gagtcatgaa cctggccggc tgccggtgct ctcaccggcc
    13501 agtatccgtc attatctgat gttgacggat actgtagtat ccactaggat ttcctcatgg
    13561 tggataccag ccgacgaccg cccctgttca ccccgcaccg gcgcgcgctg acggtgctgt
    13621 acgccgacct ggagagccac gcgcttggcc agcacgaggt cttcgtcggc accgccggct
    13681 cggtgattga gcgcaagaat gcctccgggt ttcgctttta cgcccaccag ttctatgacg
    13741 gggagggtcg caagcgcgag cgctatctgg ccgggcccgt gggtgacccg aaggccgatg
    13801 ctgccgccgc cgatctgcgc gagcgtatca aggaactcaa ggagatcgta ccctcgctgc
    13861 gcctgctggg gcgggaggga ttcaatctgg tcgacgccag gacttatgcc accctggcca
    13921 gcctgcacaa tcacggggtg ttcggggccg gcgggatgct catcggctcg cacgcctacg
    13981 gggtactgct gaaccgcctg ggcgtccgtg cgacgccgta tgccacggaa gacattgatg
    14041 tcgcccggcg cgaagcgctc gcatttgaaa agcagcccga acaaggcctg ctcgatatgc
    14101 tgaaggattc cggaatctcc tttgtcgaag tcccatcact taatcgcaag aaaccgccga
    14161 cttctttcaa acagcgcggc cgatcgcggt ttcatgtcga tctgctggtg ccgtcggcaa
    14221 acg
//
LOCUS       DASWHU010000010         9955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2220712, whole genome shotgun sequence.
ACCESSION   DASWHU010000010 DASWHU010000000
VERSION     DASWHU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220712"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..458)
                     /locus_tag="VGB88_00365"
     CDS             complement(132..458)
                     /locus_tag="VGB88_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645917.1"
                     /translation="MAIANVRQDFDRAMMGVYRSAKAECGYNARLFLRMLHEHGGLGT
                     AKRLLAVDHAQYGFEKLWECGRLDLTVECLVLGPKYRDLFDAADLDRARKRLRDHGFD
                     PARCEG"
     gene            complement(480..2795)
                     /locus_tag="VGB88_00370"
     CDS             complement(480..2795)
                     /locus_tag="VGB88_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9645918.1"
                     /translation="MVTFGIGQPLKRKEDLRLLTGRGRFIADLDRAGEAHAWFVRSAH
                     AHGTVRAIDTRAARAAPGVLAVYTAADIAGALGPLAVTARPKGRDGVAARAPNVLMLT
                     DGRVRYVGDTVAMVVAECAEAASAAAELVTVDIAPLPVVTDAAAALAPGAPQLWDEVP
                     GNLCLDWEAGDAGAVEAGFANAARIVALEAANNRVVIAAMETRGALGWVDPDSDRYVL
                     HTASQMPHPLRRELAAIFGEPEARFHVLVEDVGGGFGIKNSVYREHALVLWAARRLGR
                     PVKWIGERTDAFLTDYHGRDRVTRAELALDADGHFLAIRIEVVANLGAHLAARGALPP
                     TANTPALSGVYRTPALYARVRAVFTNTAPTEVYRGAGRPELLHLLERLVDVAAFDTGI
                     DRIELRRRNTLRPEELPFATPLGLTYDAGRYAETLDLGLARADHAGFAARRAESQRRG
                     LLRGFGLANFVERCGHGVDDSAELRIEADGTATVLTGTMSNGQGHETAFAQIAGDFLG
                     IEPARVRVVQGDTDLVREGKGTGGSRSISLGGACLTVAAAALIAKAKPLAGHLLEAAS
                     ADIEFADGRFRIAGTDRALDWAALAAAAADGGYPDDLDAKLDAIGRFSPQNHTYPNGC
                     HCCEVEVDPETGAVRILRYTVVHDFGRVLNPLLLAGQVHGGVAQGLGQAVFEHVAYDP
                     ESGQLLTGSFMDYCVPRADQLPAIDLETVATPSPSNPAGFKGCGEAGAAGAPPAVVNA
                     VVDALAAYGVRHLDMPLTPERVWRAIRAARQ"
     gene            complement(2809..3957)
                     /locus_tag="VGB88_00375"
     CDS             complement(2809..3957)
                     /locus_tag="VGB88_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX9645919.1"
                     /translation="MADVESPNRDLIGSPGSRWRLSTPALVLDLDVMERNIARMAAHC
                     RATGQALRPHGKSHKSAAIARAQAAAGAIGICCATLREAEAMVGAGLAGVLITSPPTG
                     AAKLARLMVLHAAADEATVVTDTPEHVDALAAAVSAAGTGRPLKVVVDFDVGSHRTGA
                     ASEADVVALARRIDAAPALAFAGVQAYYGHLQHIEDYGRRAGEVRAQAARLKSAVAAL
                     GEAGLAPAIVTGGGTGTHDLDHREGVFTELQAGSYLFTDVQYNQVALRAGEPRPFEPS
                     LFVHATVVNNVHRAHAIIDAGLKSFATDGPPPEFSAGTPVGASYRFFGDEHGAVVYGE
                     ANARLEVGAPVACLVPHCDPTVNLYDAYHCVRGDTLVDIWPVDARGNP"
     gene            4197..4541
                     /locus_tag="VGB88_00380"
     CDS             4197..4541
                     /locus_tag="VGB88_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX9645920.1"
                     /translation="MAWTEETVKVLAAMWAEGRSARDIAERVGTTRNAVIGKANRLGL
                     SHMSKPRPAKIEAKPRAPGSLDLNERMCRWPIGHPGDQDFHFCGNPRIAGRPYCEAHC
                     AMAYRGKAVEAA"
     gene            complement(4611..5438)
                     /locus_tag="VGB88_00385"
     CDS             complement(4611..5438)
                     /locus_tag="VGB88_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645921.1"
                     /translation="MASAEPREGYRRVVVALDARPASRGALDAAAALAARFQAELVGL
                     FIEDLNLLNMSALPFTRLAGAGPLSSGVDRATVERLLRRASGEMRAMVARLAEQAPVP
                     WSFRVVRELAGRALIAAAGEAGDLVVVEETALAGQLRPFVAGARASVLCLRTGPAATG
                     AEVILPWRADTESAHALRAAAALAAASRRPLAVLLPGDPAAARAAESRIREALAGTGI
                     EARLIRPEAGRRTLAEIARRHPGSTIVWPRRGAVEAGADIELEGIIELDHCSVLVVR"
     gene            complement(5425..7860)
                     /locus_tag="VGB88_00390"
     CDS             complement(5425..7860)
                     /locus_tag="VGB88_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9645922.1"
                     /translation="MARPKPLKPAALRRRCNRSLLKFRTTGELAGLEQVIGQPRALEA
                     LAFGIGIRRPGFNMFAFGPADTGLREVVRSSIEVQAAHEPVAEDWVYVFNFDDPGKPR
                     AISLPPGRGGAFRADMDRLVEDLRSAIRSTFETDEYRARAQAIEDEVKEQHQTALEQI
                     EGRARDNSIALVRTPLGMGFAPVRGDEVIDPEVFAKLPAEERERITRTIGELQEQLQA
                     ALQQLPRLLHQAREKVRALNREVTEFAVGHLIDALRVKYGDIDEVVEHLNSVQRDVVA
                     NAALFRDGGDGGVPAAAAQAQAQAQPFASASLRRYQVNLLVDHGADGGAPIVEEPDPS
                     YQNLIGRVEHMAELGTLVTDFSLIRPGALHLANGGYLVLDARKLLLRPYAWEGLKEAL
                     RAGEVRIESLGQIFSLISTVSLEPEHIPIRVKVVLMGEPLLYYLLQYYDPEFGELFKV
                     AVDFDNRMPRDDDTTLMYARMIASEARKAGLKALDREAVALVIEESARLAGDGERLST
                     LIRQVTDMLCEADHWAAKRRARAIGADDVRAAIEARRRRLDRLSERMREEIERGTVLI
                     ETDGTAVGRINGLAVMQLGEFMFGKPSRITARVRMGRGEVIDIEREVKLGGATHSKGV
                     LILSNFLAARYSGDYPLSLSASLAFEQSYGGVEGDSASSTELYALISALAEAPVDQGL
                     AVTGSVNQYGEVQAIGGVNEKIEGFFELCRARGLTGRQGVLIPASNVKHLMLREEVVA
                     ACAEGRFHVFPVATVDQGIEILTGVAAGRRGDDGSYPDGTINRRVVDRLREYADKRRA
                     FAIPERDRDDGER"
     gene            8041..8529
                     /locus_tag="VGB88_00395"
     CDS             8041..8529
                     /locus_tag="VGB88_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9645923.1"
                     /translation="MATRLPSLWGRSWLTGEGRDEPFAAIHRDIDRMLDDFTRGWRLP
                     AVAGRAPMLAPAVDVSETEDAIEVSAELPGIAEKDIEVEVAGNTLTIKGEKKEEKEEK
                     KKDYHLVERTFGSFQRTLPMPFDIDPDRIKANFADGVLTVSVPKPAAAKEKVKKIKLT
                     RG"
     gene            8665..9195
                     /locus_tag="VGB88_00400"
     CDS             8665..9195
                     /locus_tag="VGB88_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645924.1"
                     /translation="MGGLSTQEAGGGRLEQAFAAEERAGLRLAAVGRAAALAVIAIWL
                     PIAVPTPAVYYIEAVVLAIAASGLANWRLATRYPRQRWLGYAFAALDAALTAAGLVLV
                     PAAVIGDWPAQMTLRNGTFDYYFVYLALVALSYAPRLMLWAGACSALAWVLAVQWALA
                     QPGTRLDQPAEMCGIM"
     gene            9257..>9955
                     /locus_tag="VGB88_00405"
     CDS             9257..>9955
                     /locus_tag="VGB88_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645925.1"
                     /translation="MNTSHTAKLAAGRILSIFFVVGGWLIAGGRAVLDLIGYATLGED
                     AKIAEGLLARFLDWLISLPWWAPWGFALTATVLLIWASWPKSAATSSLATTPTQPDPP
                     AATIAAITDLIKQIRLQEKQNLTRRDTEKTSDENKYDAQGLGYLILLQQVGTNILHKW
                     GFKNDVEYGIWVHQQDGWIKNAEYVLRKYFKDEDYNWFSTLGVFDAPSCQNPYNADHS
                     HKYSMHWERLRRIGK"
BASE COUNT         1499 a         3480 c         3544 g         1432 t
ORIGIN      
        1 cgacaggccg cagcaacagg ccgaatacat gaccgctcca gactctttgc caaaaccgct
       61 tctcgcccct tgccagaggg gggccgtcca tacatgtcaa tgaagatgcc gcccctgcca
      121 tccgtggccc gtcacccctc gcagcgggcg gggtcgaagc cgtggtcgcg gagccgcttg
      181 cgcgcgcggt cgaggtcggc ggcgtcgaac aggtcacggt acttcgggcc tagcaccagg
      241 cactcgacgg tgaggtcgag gcggccgcac tcccacaact tctcgaagcc gtattgggca
      301 tggtccacgg cgagcagccg cttggcggtg ccgaggccgc cgtgctcgtg caacatgcgc
      361 aggaacagcc ttgcattgta accgcactcg gccttggccg agcggtaaac gcccatcatg
      421 gcgcggtcga agtcctggcg gacgttggct atagccaccc ccgcactgcg cgtgctcact
      481 tactgcctgg ccgcgcggat cgcgcgccag acgcgttcgg gggtgagcgg catgtcgagg
      541 tggcgcacgc cgtaagcagc cagcgcatcg accaccgcat tcaccaccgc cggcggcgcg
      601 ccggccgcgc ccgcctcgcc gcagcccttg aacccggccg ggttcgacgg cgagggcgtc
      661 gccaccgtct cgaggtcgat cgccggcagc tggtcggcgc gcggcacgca gtagtccatg
      721 aacgagccgg tgagaagctg cccggactcg ggatcgtagg cgacgtgctc gaacaccgcc
      781 tggccgaggc cctgggcgac gccgccatgg acctggccgg cgagcagcag cgggttgagc
      841 acccggccga agtcgtgcac cacggtgtag cgcaggatgc gcaccgcgcc cgtttcgggg
      901 tcgacctcga cctcgcagca gtggcagccg ttgggatagg tgtggttctg cggcgagaac
      961 cggccgatcg cgtcgagctt ggcgtcgagg tcgtcgggat agccgccgtc ggcggccgcg
     1021 gcggcgagcg ccgcccagtc gagggcgcgg tcggtgccgg cgattcggaa gcgcccgtcg
     1081 gcgaactcga tgtcggcgga cgccgcctcg agcaggtggc cggcgagcgg cttggctttg
     1141 gcgatcagcg ccgccgccgc caccgtcagg caagcgccgc cgagcgaaat cgagcgcgag
     1201 ccgccggtgc ccttgccctc gcgcaccagg tcggtgtcgc cctggacgac gcgcacccgc
     1261 gccggctcga tgccgaggaa gtcgccggcg atctgggcga aggcggtctc gtggccctgg
     1321 ccgttcgaca tggtgccggt gagcacggtg gcggtgccgt cggcctcgat gcgcagctcc
     1381 gcgctgtcgt cgacgccgtg gccgcagcgc tcgacgaagt tggcgaggcc gaagccgcgc
     1441 agcaggccgc ggcgctggga ctcggcgcgc cgtgcggcga agcccgcgtg gtccgcgcgg
     1501 gcgaggccga ggtcgagcgt ctcggcgtag cggccggcgt cgtaggtaag cccgagcggg
     1561 gtcgcgaacg gcagctcctc gggccggagc gtgttgcgcc ggcgcagctc gatgcggtcg
     1621 atgccggtat cgaaggcggc gacgtcgacc aggcgctcga gcaggtgcag cagctcgggc
     1681 cgcccggcac cgcgatagac ctcggtcggc gcggtattgg tgaacacggc gcgcacccgg
     1741 gcataaagtg ccggcgtgcg gtagacgccg ctcagcgccg gggtgttggc ggtcggcggc
     1801 agcgccccgc gcgcggcgag gtgcgcgccc agattggcca ccacctcgat gcggatggcg
     1861 aggaagtggc cgtcggcatc gagcgcgagc tcggcccggg tgacgcggtc gcggccgtga
     1921 tagtcggtga ggaaggcatc cgtgcgctcg ccgatccact tcaccggccg gcccaggcgg
     1981 cgtgccgccc acagcaccag cgcgtgctcg cggtagaccg agtttttgat cccgaagccg
     2041 ccgccgacgt cctcgaccag cacgtggaag cgcgcctcgg gctcgccgaa gatggcggca
     2101 agctcgcggc gcagcgggtg cggcatctgc gaggcggtgt gcagcacgta gcggtcgctg
     2161 tcgggatcga cccagccgag cgcgccgcgg gtctccatcg ccgcgatcac gacccggttg
     2221 ttggcggcct cgagcgcgac aatgcgcgcg gcgttagcga acccggcctc gaccgcgccg
     2281 gcatcgcccg cctcccagtc gaggcacagg ttgcccggca cctcgtccca cagctgcggc
     2341 gcgcccggcg cgagcgcggc ggcggcgtcc gtgaccaccg gcaagggcgc gatgtcgacc
     2401 gtgaccagct ccgccgccgc actcgccgcc tcggcgcact cggcgaccac catcgccacc
     2461 gtgtcgccga cgtagcgcac ccgcccgtcg gtgagcatca gcacgttcgg cgcgcgcgcg
     2521 gccacgccgt cgcggccctt ggggcgcgcg gtgaccgcga gcgggccgag cgcgccggcg
     2581 atgtcggccg cggtgtagac cgccagcacc cccggcgccg cgcgcgccgc gcgggtatcg
     2641 atcgcgcgca ccgtgccgtg ggcatgcgcc gagcggacga accaggcatg cgcctcgccc
     2701 gcccggtcga gatcggcgat gaaccggccg cggccggtga gcaggcgcaa atcctccttg
     2761 cgcttaagcg gctggccgat gccgaacgtc accatcgccg cctcaggctc aggggttgcc
     2821 gcgcgcgtcc accggccaga tgtcgaccag ggtgtcgccg cgcacgcagt gataggcgtc
     2881 gtacaggttc acggtcgggt cgcagtgcgg caccaggcag gcgaccggcg cgccgacctc
     2941 gagccgggcg ttggcctcgc cgtagaccac cgcgccgtgc tcgtcgccga agaagcggta
     3001 gctcgccccg accggggtgc cggcgctaaa ctcgggcggc gggccgtcgg tggcgaagct
     3061 cttgaggccg gcgtcgatga tcgcgtgcgc gcggtggaca ttgttgacca cggtggcgtg
     3121 gacgaacagc gacggctcga acgggcgcgg ctcgccggcg cgcagcgcga cctggttgta
     3181 ctggacgtcg gtgaacaggt aggagcccgc ctgcagctcg gtgaatacgc cttcgcgatg
     3241 gtcgaggtcg tgggtgccgg tgccgccgcc ggtgacgatc gcgggcgcga gcccggcctc
     3301 gccgagcgcg gccaccgccg acttgagccg cgccgcctgg gcgcgcacct cgcccgcgcg
     3361 ccggccgtag tcctcgatgt gctggagatg gccgtaatag gcctggacgc cggcaaaggc
     3421 gagcgcgggc gcggcatcga tgcggcgggc gagcgcgacc acgtcggcct cgctggcggc
     3481 tccggtgcgg tggctgccga cgtcgaagtc gaccaccacc ttgagcggcc ggccagtacc
     3541 cgccgcgctg accgcggccg caagcgcatc gacatgctcg ggcgtgtcgg tgaccaccgt
     3601 cgcctcgtcc gccgccgcgt gcagcaccat cagccgcgcg agcttggccg cgccggtcgg
     3661 cggcgaggtg atcagcaccc cggcgagccc cgcgccgacc atcgcctcgg cctcgcgcaa
     3721 ggtggcgcag cagatgccga tcgcgcccgc cgcggcctgg gcgcgggcga tggcggcgga
     3781 cttgtggctc ttgccgtggg gcctcagcgc ctggccggtg gcgcggcaat gcgccgccat
     3841 gcgggcgatg ttgcgctcca tgacgtcgag gtcgagcacc agcgccggcg tgctcagccg
     3901 ccagcggctg ccgggcgacc cgatcaggtc ccggttgggc gactcgacgt cggccatggc
     3961 gtgcctcccc ccagccggaa cggcgatccg ccgcgcgtgc cggtgcgtgt ggttagcagc
     4021 ccgcccgccc gcccgcaagc aggccggcgc cccagccggc cggggcggtg catggaccgg
     4081 tggacaagct gtgcaaaagc cgaattcctt gtggataacc acaaaatcta gtagatcgtg
     4141 gggccgattt tcactacatg taccagaagt ttcaactcgg cagggggatc ggtgcgatgg
     4201 catggaccga ggaaacggtc aaggtgctgg cagccatgtg ggccgagggc cgcagcgcgc
     4261 gcgacatcgc ggaacgcgtg gggacgaccc gcaacgccgt gatcggcaag gcaaatcggc
     4321 tcggcctgtc gcacatgtcg aagccgcggc cggcgaaaat tgaggccaag ccgcgagccc
     4381 ccggctcgct cgacctcaac gaacgcatgt gccggtggcc gatcggccat cccggcgacc
     4441 aggacttcca cttctgcggc aacccgcgca tcgccgggcg gccctattgc gaggcgcact
     4501 gcgcgatggc ctatcgcggc aaggcggtcg aagccgcctg atccgccgcc gcggcgccgt
     4561 cgccgggcgg gagcgcgcgc cgcgccccgc ccgcggtctc gcccggccgc tcagcggacc
     4621 accagcaccg agcagtggtc gagctcgatg atcccctcga gctcgatgtc cgcgccggcc
     4681 tcgaccgcac cccggcgcgg ccagacgatg gtcgatcccg ggtgccgccg ggcgatctcg
     4741 gcgagggtcc gccggccggc ctcgggccgg atgaggcgcg cctcgatgcc ggtgccggcc
     4801 agcgcctcgc ggattcgaga ctccgccgcg cgcgccgccg ccggatcgcc cggcagcagc
     4861 accgccagcg gtcgccggga cgccgcggcg agcgcggctg ccgcgcgcag cgcgtgcgcc
     4921 gactcggtgt ccgcgcgcca gggcaggatg acctcggcgc cggttgcggc gggtcccgtg
     4981 cgcaggcaca ggaccgaggc gcgcgcgccg gccacgaacg ggcgcagctg gccggcgagc
     5041 gcagtttcct cgaccaccac caggtcgcct gcctcgcccg cggcggcgat cagcgcccgg
     5101 ccggcgagct cgcgcacgac gcggaacgac cacggcaccg gcgcctgctc ggccaggcgc
     5161 gcgaccatgg cgcgcatctc gcccgaggcg cggcgcagca gccgctcgac cgtcgcccgg
     5221 tcgacgccgc tcgacagcgg ccccgccccg gccaggcgcg tgaacggtag cgcgctcatg
     5281 ttgagcaggt tgaggtcctc gataaacagc ccgaccagct cggcctgaaa gcgcgccgcg
     5341 agcgccgccg cggcatcgag cgcgccgcgg ctggcggggc gcgcgtcgag cgccaccacc
     5401 acccggcgat agccctcgcg cggctcagcg ctcgccatcg tcccggtccc gttcggggat
     5461 ggcgaaggcg cgccgcttgt cggcgtattc gcgcagccgg tcgaccacgc gccggttgat
     5521 ggtgccgtcc ggatagctgc cgtcgtcgcc gcgccggccg gcggccacgc cggtcaggat
     5581 ctcgatcccc tggtcgacgg tcgccaccgg gaagacgtgg aagcggccct cggcgcaggc
     5641 ggcgaccacc tcctcgcgca gcatcaggtg cttgacgttc gaggcaggga tcagcacgcc
     5701 ctggcggccg gtcagcccgc gcgcacggca cagctcgaag aagccctcga tcttctcgtt
     5761 gaccccgccg atcgcctgca cctcgccgta ctggttgacc gagccggtga ccgcgagccc
     5821 ctggtcgacc ggcgcctcgg cgagcgccga gatcagcgca tagagctcgg tggacgaggc
     5881 gctgtcgccc tcgacgccgc cgtaggactg ctcgaacgcc aggctcgcgc tcaacgacag
     5941 cggatagtcg cccgagtagc gggcggcgag gaagttggac aggatcagca cgcccttgga
     6001 atgggtggcg ccgccgagct tgacctcgcg ctcgatgtcg atcacctcgc cgcggcccat
     6061 gcgcacccgg gcggtgatcc gcgacggctt gccaaacatg aactcgccga gctgcatcac
     6121 tgccaggccg ttgatccgcc cgaccgcagt gccgtcggtc tcgatcagga ccgtgccgcg
     6181 ctcgatctcc tcgcgcatcc gctccgacag ccggtcgagg cggcgccggc gcgcctcgat
     6241 cgcggcgcgc acgtcgtcgg cgccgatggc gcgcgccctg cgcttcgctg cccagtggtc
     6301 ggcctcgcac agcatgtcgg tgacctggcg gatcagcgtc gacagccgct cgccgtcgcc
     6361 ggcgagcctg gcgctctcct cgatcaccag cgccaccgcc tcgcggtcga gcgccttcag
     6421 ccccgccttg cgcgcctcgc tggcgatcat gcgcgcatac atgagcgtgg tgtcgtcgtc
     6481 gcgcggcatg cggttgtcga agtcgaccgc gaccttgaac agctccccga actctgggtc
     6541 atagtactgc aacaggtagt acagcaacgg ctcgcccatc agcaccacct tgacccggat
     6601 cgggatgtgc tcgggctcaa gcgagacggt gctgatgagg ctgaagatct ggcccagcga
     6661 ttcgatccgc acctcgccgg cgcggagcgc ctccttcagc ccctcccagg catagggccg
     6721 caacagcagc ttgcgcgcat cgagcaccag atagccgcca ttggcgagat gcagcgcgcc
     6781 cgggcggatc aggctgaagt cggtgaccag ggtgccgagc tcggccatgt gctcgacgcg
     6841 gccgatcagg ttctggtagc tggggtcggg ctcctcgacg atcggcgcgc cgccgtcggc
     6901 gccgtgatcg accagcaaat tgacctggta gcgcctgagc gaggcggacg cgaacggctg
     6961 cgcctgcgcc tgcgcctgcg ccgccgccgc gggcacgccg ccgtcgccgc cgtcgcggaa
     7021 cagcgccgcg ttcgcgacca cgtcgcgctg gacgctgttc aggtgctcga ccacctcgtc
     7081 gatgtcgcca tacttgaccc tgagcgcgtc gatcaggtgg ccgaccgcga actcggtcac
     7141 ctcgcggttg agcgcgcgca ccttctcgcg cgcctgatgc aacagccgcg gcagctgctg
     7201 gagcgccgcc tgcagctgct cctgaagctc gccgatggtg cgggtgatgc gctcgcgctc
     7261 ctcggccggg agcttggcga acacctcggg gtcgatcacc tcgtcgccgc gcaccggcgc
     7321 aaagcccatg ccgagcggcg tgcgcaccag tgcgatgctg ttgtcgcgcg cgcggccctc
     7381 gatctgctcg agcgcggtct ggtgctgctc cttgacctcg tcctcgatcg cctgggcgcg
     7441 ggcgcgatac tcgtcggtct cgaaggtcga gcggatcgcg ctgcgcaggt cctcgaccag
     7501 gcggtccatg tcggcgcgga acgcgccgcc gcgcccgggg ggcagcgaga tcgcgcgcgg
     7561 cttgcccggg tcgtcgaagt tgaacacgta gacccagtcc tcggccaccg gctcgtgcgc
     7621 ggcctggacc tcgatcgagc ttcgcaccac ctcgcgcagc ccggtatcgg cgggcccgaa
     7681 agcgaacatg ttgaagccgg ggcggcggat gccgatgccg aaggcgagcg cctcgagcgc
     7741 gcgcggctgg ccgatcacct gctcgaggcc ggcgagctcg ccggtggtcc ggaacttgag
     7801 cagggaccgg ttgcagcgcc gcctgagcgc cgccggcttc aagggcttgg gccgcgccat
     7861 gcagtctgct cctcgtcgct agccggggca tcatgtggga ccgcgccgcg ctgtcaagcg
     7921 agaccgggcg cgccgcgcac gcatcactga tctgcatcaa ggactggcaa tcgggcgccg
     7981 cctaaccatg tcggccggtg cggaaacgca atccgatcgt ttcatccagg aggagtcgcc
     8041 atggcaacac gattgccgtc cctctggggc cgctcgtggc tgacgggcga aggccgcgac
     8101 gagccctttg ccgctatcca ccgcgacatc gaccgcatgc tcgacgactt cacccgcggc
     8161 tggcgcctgc cggcggtggc aggccgcgcg ccgatgctcg cgccggcggt cgacgtgagc
     8221 gagaccgagg atgcgatcga ggtcagcgcc gagcttcccg gcatcgccga gaaggacatc
     8281 gaggtcgagg tggccggcaa cacgctgacc atcaagggcg agaagaagga ggaaaaggag
     8341 gagaagaaga aggactacca cctggtcgag cgcacgttcg gctcgttcca gcgaaccctg
     8401 ccgatgccct tcgacatcga tcccgaccgg atcaaggcca acttcgccga cggcgtgctg
     8461 acggtgagcg tgcccaagcc ggcggcggcc aaggagaagg tcaagaagat caagctgacg
     8521 cgcggctgag ccgcgcgccg cggcccgccg gacggcctct cccctccgcc ggcgggccgc
     8581 cgctgcgcct tgattcgggc gcccgcccgg cgtctgctgc gccgaccgtg acggggcacg
     8641 gcgcggcagg cggaggcacg ggcgatgggc ggactctcga ctcaggaggc cggcggcggg
     8701 cggctggagc aggcgttcgc ggccgaggaa cgagccggtc tcaggctggc cgcggtgggc
     8761 cgcgcggcgg cgctggcggt gatcgcgatc tggctgccga tcgcggtgcc gacgcctgcg
     8821 gtctattaca tcgaggcggt ggtccttgcc atcgccgcga gcggcctggc caactggcgg
     8881 ttggccacgc gctatccgcg gcagcgttgg ctgggctacg ccttcgccgc gctcgacgcc
     8941 gcgctgaccg ccgccggact ggtgctggtt ccggcggcgg tgatcggcga ctggccggcg
     9001 cagatgacgc tccgcaacgg caccttcgac tactacttcg tttacctcgc cctggtcgcg
     9061 ctgagctacg cgccgcggct gatgctgtgg gcgggcgcgt gctctgcgct cgcctgggtg
     9121 ctggcggtgc aatgggccct cgcccagccc ggcacgcggc tcgaccagcc ggcggagatg
     9181 tgcggaatta tgtagcaagc acacctagtg ccaggggact cggcaacaat gcgattttcg
     9241 ggcatcctcg ccggtcatga atacaagcca tacggccaag ctggcggctg gccgtatcct
     9301 gagcattttc tttgtagtgg gcggctggct aatagctggc ggcagagccg tgctcgactt
     9361 gatcggctac gcgacgttgg gggaagatgc caaaatcgcc gaaggtttat tggcgcgatt
     9421 cctcgattgg ctgatatcgc tcccatggtg ggcaccatgg ggattcgcgc taacagcaac
     9481 ggttttgctc atctgggctt catggcccaa aagtgccgct acttcatctt tggcaacgac
     9541 gcctactcag cctgatccgc ctgccgccac tattgcggca atcaccgatc taattaaaca
     9601 aatccgttta caagaaaagc aaaacctaac tcgccgtgac actgaaaaaa catcagatga
     9661 aaacaagtat gatgcacaag gtttaggtta tttaattcta ttgcaacagg tcggaactaa
     9721 tattctccat aaatggggct ttaagaatga tgtggaatat ggaatatggg tgcatcaaca
     9781 ggatggatgg attaagaatg cggaatatgt attaaggaaa tatttcaaag atgaggatta
     9841 taattggttt tctacattag gcgtctttga cgcgcctagt tgccagaatc catataatgc
     9901 tgatcatagc cacaagtata gtatgcattg ggaacggttg agaagaatag ggaaa
//
LOCUS       DASWHU010000011         6436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2220780, whole genome shotgun sequence.
ACCESSION   DASWHU010000011 DASWHU010000000
VERSION     DASWHU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220780"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VGB88_00410"
     CDS             complement(<1..1724)
                     /locus_tag="VGB88_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEX9645926.1"
                     /translation="MASVTDTARVERSRARSRAGGRAARVPAPVPEVKARPKPQDGGP
                     GEGRPARRARRRFSALTQRILAVNLLALGIVVGGVFYLDQYQRGLIEGRLEALVTQGL
                     IVAGALGEAALPRDPTALQGMDPAVAGELLQRLITPLKTRARLFGNDGELLVDSRRLS
                     SASAGVRIRELPPPELPGRLDPLVDALSDTVDRLLMGDLPHYVEGSYQRAEHYAEVVH
                     ALHGDIDAEARRDSDGKIVLNVAVPVRRFKAVQGALMLTAGTEDIDAKVRSVRGDILK
                     LCALAVAITVLLSLYLAGTIARPIRQLAAGAERVAGGRGGPEELPDLSRRDDEVGELS
                     VALRAMTDALHWRLDAIARFAADVAHELKNPLSSLRSAVDTIARIDDPATRERLLSIL
                     ADDIERMDRLISDISAASRLDAELSRATMAPVDLAALLEALVSLYRDACTGPEITLEV
                     AAAGAIDVRGVEDRLAQVFRNLIDNAISFSPPAGAITVRLERRRDGAVVTVEDQGPGI
                     PEGKTGAIFERFYSERPSGEKFGTHSGLGLSISKQIVDAHHGTIRAENRRGDGPGTAA
                     GARFIVWLP"
     gene            complement(1693..2400)
                     /locus_tag="VGB88_00415"
     CDS             complement(1693..2400)
                     /locus_tag="VGB88_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9645927.1"
                     /translation="MKPTIAIVDDDQNILTSVSIALDAEGFQTRTYTDGERAINGLLK
                     EPANLVVLDIKMPRMDGHEVLTRLRRESTVPVIFLTSKDDEVDELLGLKLGADDYITK
                     PFSVRLLIERIRALLRRVELGQAPADGAAEGQPPVLRRGELVLDPARHLCTWKGREVQ
                     LTVTEFLILQALATRPGHVKSRNQLMDAAYGDQVYVDDRTIDSHIKRMRRKFRDVDAD
                     FAHIETLYGIGYRYSEG"
     gene            2563..3312
                     /locus_tag="VGB88_00420"
     CDS             2563..3312
                     /locus_tag="VGB88_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX9645928.1"
                     /translation="MTATVAAQARRLVRACDRAALATAQHDADGWPYASLVMCASDFA
                     GAPLMLLSDLAEHVRNLKRDGRAALLFDATADLDEPLAGARLTVLGRIERCAEPAARA
                     RYLARHPSAAGYAGFADFGLWRLEPVRAHLVAGFGAIHWVDGDALLVDAAGAEALAAA
                     EGEIVAHMNTDHGDALSAYAAGLLGRGGGGWTMTGIDPEGLDLRRGAETARIAFAAPV
                     DGPGAARAELVRLAGVARAGLAGDGAAGGAR"
     gene            3309..4400
                     /locus_tag="VGB88_00425"
     CDS             3309..4400
                     /locus_tag="VGB88_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX9645929.1"
                     /translation="MTEPVIVDYRSFRPRIGRGARFGEGSALIGRVTLGEGVTLGRLT
                     TLRADGERIDVGRGCTFLDRATVHISDGLLPSVVGDRVTVGRYALVHACTVGDDCVLG
                     DGAVVMDAARLGAGAVIAAGALVPPAKELAGGTLYAGNPARPVRALDARERDALRAAV
                     LAGTPDAALGACGLPPLDMAPFGPPGALAGRAPAIDDAAYLAPTALVAGAVSVAAHAS
                     IWFATACRAEGAEIRIGARSNVQDNSILVARDGGDIVIGKDVTVGHNVRMGACRIGDG
                     CLIGMGSEIGDGVVVEDGGAIGARALVAPGTVIEAGWIWAGRPARAFRPLKPEEVEFF
                     SRGTAVYVGYAATYLAEAQNVGKDEHSDR"
     gene            complement(4590..5771)
                     /locus_tag="VGB88_00430"
     CDS             complement(4590..5771)
                     /locus_tag="VGB88_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019277300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645930.1"
                     /translation="MSCIGKRVLLSVAMAACVVAPTGIRADSVPHTGEQSPPVLLGTS
                     GSTQAGVVIDGIEYCYAGTLGGGVEDANGNPHILSNYHVLAHPNALFPGEPIVHPALL
                     DNDCSTESADYVTVGHLSNYVPIKFCKAVGPLFMKCSNNTVDAAVASFEGGQVNPDGE
                     VLDIGFIGTAAKSANLGMEVQKSGRTTGHTVGTVAAVNVTVLVCYDEPCSDPANVAQF
                     VNQVRVDGSFSAPGDSGSLVVECVDNPPDGCDDFPAPVGLLFAGGTNTTFFNRFEDVA
                     SALAVAPLGCDSACFDAGDGGGGGGGGGKGPGGGGPPGRSSSTDLGVERAREVQERHT
                     PELLRRRGVLGTGLAVDDSGEPVIEVYIRAPMVAMDDPIPSDLDGIQLRVIETGQIRA
                     Y"
     gene            complement(6073..>6436)
                     /locus_tag="VGB88_00435"
     CDS             complement(6073..>6436)
                     /locus_tag="VGB88_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645931.1"
                     /translation="GAFLTIFAIAVPLMALAVAFQIAIALASRSMKEAQIYLGLLPLV
                     PALPGMVMVFSPLRSTLGVASVPLMGQLAQFNQLVGGLAVPLGQIALCAAVTLALTAL
                     IFWQAVRLFQREKLLFAS"
BASE COUNT          950 a         2295 c         2256 g          935 t
ORIGIN      
        1 ggcagccaga cgatgaagcg ggcgccggcg gccgtgccgg gcccgtcccc gcggcggttc
       61 tcggcgcgga tggtgccgtg atgggcatcc acgatctgct tggagatgct caggcccaac
      121 cctgagtggg taccgaactt ctcgcccgag ggccgctcgc tgtagaagcg ctcgaagatg
      181 gcgccggtct tgccctcggg gatgcccggg ccctggtcct cgaccgtgac cacggcgccg
      241 tcgcgccggc gctcgagccg cacggtgatg gcgccggcgg gcgggctgaa cgagatcgcg
      301 ttgtcgatca ggttgcggaa cacctgggcc aggcggtcct cgaccccgcg cacgtcgatc
      361 gcgcccgcgg ccgcgacctc gagcgtgatc tcgggcccgg tgcaggcgtc gcgatagagc
      421 gagaccagcg cctcgagcag cgcggcgagg tcgaccggcg ccatggtcgc gcgcgacagc
      481 tcggcatcga gccgcgaggc cgccgagatg tcgctgatca gccggtccat gcgctcgatg
      541 tcgtcggcga ggatcgacag caaccgctcg cgggtcgccg ggtcgtcgat gcgggcgatg
      601 gtatcgacgg cgctgcgcag gctcgacagc gggttcttga gctcatgcgc cacatcggcg
      661 gcgaagcgcg cgatcgcgtc gagccgccag tgcagggcat cggtcatcgc ccgcagcgcc
      721 accgacagct cgccgacctc gtcgtcgcgc cggctcaaat cgggcagctc ctcgggcccg
      781 ccgcggccgc ccgccacccg ttccgcgccg gcggcgagct ggcggatcgg ccgggcgatg
      841 gtgccggcga ggtacagcga cagcagcacc gtgatcgcga ccgcgagcgc gcacaattta
      901 aggatgtcgc cgcgcacgct gcgcaccttg gcgtcgatgt cctcggtgcc tgcggtcagc
      961 atcagcgcgc cctggaccgc cttgaaccgg cgcaccggca cggcgacgtt gagcacgatc
     1021 ttgccgtccg aatcgcggcg cgcctcggcg tcgatgtcgc cgtgcagcgc gtggaccacc
     1081 tcggcgtagt gctcggcgcg ctggtagctg ccctccacat agtgcggcag gtcgcccatc
     1141 agcagccggt cgaccgtgtc cgacagcgcg tcgaccagcg ggtcgaggcg gccgggaagc
     1201 tcgggcggcg gcagctcgcg gatgcgcacc ccggcgctgg cgctcgacag ccgccggctg
     1261 tcgaccagca gctcgccgtc gttgccgaac agccgcgcgc gcgtcttgag cggggtgatc
     1321 aggcgctgga gaagctcgcc cgcgaccgcc ggatccatgc cctggagcgc ggtcgggtcg
     1381 cgcggcagcg cggcctcgcc gagcgcgccg gcgacgatca ggccctgggt gaccagcgct
     1441 tccagccggc cctcgatcag gccgcgctgg tactggtcga gatagaacac gccgccgacg
     1501 acgatgccga gggcgagcag gttgaccgcc aggatgcgct gggtcagcgc cgagaaccgg
     1561 cgccgcgcgc ggcgcgccgg gcggccctcg cccggcccgc cgtcctgggg cttggggcgc
     1621 gccttcacct ccggcaccgg cgccggcacg cgcgccgcgc gcccgccggc acggctgcgc
     1681 gcgcggctgc gctcaaccct cgctgtatcg gtaaccgatg ccataaagcg tttcgatgtg
     1741 cgcgaagtcg gcatcgacgt cgcggaactt gcggcgcatg cgcttgatgt ggctgtcgat
     1801 ggtgcggtcg tcgacataga cctggtcgcc gtaggcggca tccatcagct ggttgcggct
     1861 cttgacgtgg cccgggcggg tggcgagcgc ttgcaggatc aggaactcgg tcaccgtcag
     1921 ctgcacctcc cggcccttcc aggtgcacag atggcgcgcc gggtcgagca ccagctcgcc
     1981 gcggcgcagc accggcggct ggccctccgc cgcgccgtcg gccggcgcct ggccgagctc
     2041 gacccggcgc agcagcgccc ggatgcgctc gatcaggagc cgcaccgaga acggcttggt
     2101 gatgtagtcg tcggcgccga gcttgaggcc caggagctcg tcgacctcgt cgtccttcga
     2161 ggtcaggaag atcaccggca ccgtggattc gcggcgcagc cgggtcagca cctcgtggcc
     2221 gtccatgcgc ggcatcttga tgtcgagcac gaccagattc gccggctcct tgagcaggcc
     2281 gttgatcgcg cgctcgccgt cggtataggt gcgggtctgg aagccctcgg cgtcgagggc
     2341 gatcgacacc gaggtgagga tgttctggtc gtcgtcgacg atcgcgatgg tcggtttcac
     2401 gcctcgtccc tcacgcgcag cccgccgggc cgcgggcgcc gcccgcgacc gtcccgtgcc
     2461 accatatggt gtcggcccgg ggattgtaca cgcgcgcccc cgatgcgggt tttgccgccc
     2521 gccgagacgc gctagggtcg gcccgggatg aacgggagcg agatgaccgc aacggtggcg
     2581 gcacaggcgc gccggctggt gcgggcgtgc gaccgggcgg cgctggccac cgcccagcac
     2641 gacgccgacg gctggcccta cgcctcgctg gtgatgtgcg cctcggactt cgccggcgcg
     2701 ccgctgatgc tgctgtcgga cctcgccgag catgtccgca acctcaagcg ggacgggcgc
     2761 gcggcgctct tgttcgacgc caccgccgac ctcgacgagc cgctggccgg ggcccgcctg
     2821 accgtgctcg gacgcatcga gcggtgcgcc gagccggcgg cgcgcgcgcg ctacctggca
     2881 cgccacccct cggccgcggg ctacgccggt ttcgccgact tcggcctgtg gcgcctcgag
     2941 ccggtgcgcg cgcacctggt tgccggcttc ggcgccatcc attgggtcga cggcgacgca
     3001 cttctggtcg atgcggccgg ggccgaggcg ctggccgccg ccgagggcga gatcgtcgcg
     3061 cacatgaaca ccgaccacgg cgacgcgctg tcggcctatg ccgcgggcct gttgggccgc
     3121 ggcggcggcg gctggaccat gaccgggatc gaccccgagg gcctcgacct caggcgcggg
     3181 gcggagaccg cgcgcatcgc cttcgccgcg ccggttgacg ggcccggggc ggcgcgcgcc
     3241 gagctggtgc gcctggccgg cgttgcgcgc gccgggctgg ccggcgacgg cgccgccggc
     3301 ggcgcccgat gaccgagccg gtgatcgtcg actaccggtc gtttcgcccg cgtatcggtc
     3361 gcggcgcgcg cttcggcgag ggcagcgcgc tgatcgggcg ggtgactctc ggcgagggcg
     3421 tcacgctcgg ccggctgacg acccttcgcg ccgacggcga gcgcatcgac gtcggccgcg
     3481 gctgcacctt cctcgaccgc gccaccgtcc acatctcgga cggcctgctg ccgagcgtgg
     3541 tcggcgaccg ggtcacggtc ggccgctacg cgctggtcca tgcctgcacc gtgggcgatg
     3601 actgcgtgct cggcgacggc gcggtggtca tggacgcggc gaggctcggc gccggcgcgg
     3661 taatcgccgc cggcgcgctg gtgccgcccg ccaaggagct cgccggcggc acgctgtatg
     3721 ccggcaaccc ggcgcgcccg gtgcgcgcgc tcgacgcccg cgagcgcgac gcgctgcgcg
     3781 ccgcggtgct tgccggcacg ccggacgcag cgctcggcgc ctgcggcttg ccgccgctcg
     3841 acatggcgcc gttcggcccg ccgggagcgc tcgccggccg ggcgccggcg atcgatgacg
     3901 cggcctattt ggcgccgacc gcgctggtcg cgggcgcggt gtcggtggct gcgcacgcca
     3961 gcatctggtt cgccaccgcc tgccgggccg agggcgccga gatccggatc ggtgcgcgca
     4021 gcaacgtcca ggacaactcg atcctggtcg cgcgcgacgg cggcgacatc gtcatcggca
     4081 aggacgtgac cgtcggccac aacgtccgca tgggcgcctg ccggatcggc gacggctgcc
     4141 tgatcggcat gggctcggag atcggcgacg gcgtggtggt cgaggacggc ggcgcgatcg
     4201 gcgcccgcgc cctggtcgcg cccggcaccg tcatcgaggc gggctggatc tgggccggcc
     4261 gcccggcgcg cgcgttccgc ccgctcaagc ccgaggaggt cgagttcttc agccgcggca
     4321 ccgcggtcta tgtcggctac gccgcgacct atctcgccga ggcccagaac gtcggcaaag
     4381 acgagcacag cgatcggtaa cgttgcgcct tcagaccggc aagactccga acgcattcgt
     4441 tggtcgtgat acaggctgtg gcagcaaacc caaccacgac tgtcctctgc cgaccagacc
     4501 tgcaccgggc ctggccggac ttgccgcgaa cagatgcgct catcgacagg cgaagtggcg
     4561 acgcgcgccc gtccgaagcc gagctccgtt caataggcgc gaatctgtcc cgtctcgatc
     4621 acgcgcagct gaatgccgtc caagtcggag gggatggggt cgtccatagc gaccatcggg
     4681 gcccggatat agacctcgat aaccggctct cccgaatcgt caaccgcaag cccggttccg
     4741 aggacgcccc gacgacgaag cagttccggc gtgtgtctct cctggacctc gcgcgcccgt
     4801 tccacaccca gatcggtgga actgctgcga cccggaggac cgcctccccc tggtcctttg
     4861 ccaccaccac caccgcctcc gcctccgtcg cccgcgtcaa agcatgccga gtcgcaaccc
     4921 aacggggcga cggcaagggc cgacgccacg tcctcgaacc tgttgaagaa agtggtattg
     4981 gtgccgccgg caaacagcag tccgaccggt gcgggaaaat catcgcagcc gtcgggaggg
     5041 ttgtccacgc actcgacaac caatgacccc gaatcgcccg gcgcgctgaa cgagccgtcg
     5101 acgcgcacct gattaacgaa ctgggccacg ttcgcgggat cgctgcacgg ctcgtcgtaa
     5161 catacgagca cggtcacgtt gactgcggct accgtgccaa ccgtatggcc ggtcgtgcga
     5221 ccgctctttt gcacttccat gcccagattg gcggacttgg cggcagtccc aataaagccg
     5281 atgtcgagaa cttcaccgtc aggattcact tggccgccct cgaaggacgc aacggctgca
     5341 tcgacggtgt tgttgctgca cttcatgaac agcggcccga cggccttgca gaacttgatc
     5401 gggacgtagt tgctcaagtg accgacggtg acatagtccg ccgactccgt cgagcagtcg
     5461 ttgtccagca gcgccggatg gacgatcggc tcgcccggaa acagcgcgtt gggatgagcg
     5521 agcacatgat agttgctaag aatgtgagga tttccgttgg cgtcctcgac gcctccgccg
     5581 agggtgccgg cgtagcagta ttcgatcccg tcaatgacca caccggcttg ggtgctgcct
     5641 gaagtgccaa gcagtaccgg cggactctgc tcaccagtat gaggaacgga gtcggcacgg
     5701 atcccggtgg gcgcgacgac gcaagctgcc atcgcaacgg acagcaaaac ccttttgcca
     5761 atgcacgaca tcactttact ccatcggacc cgctgggcag cggacgcttg ccacgacgac
     5821 cgaaaccgtc cgcgccgcat ccagatcggt tgttaacatg gcacaagcaa cgctctcttg
     5881 ccggtccgga gcccatttcc gggtccggac gccgcagatg gccgtttcag gccgacctcg
     5941 cttcccatta ttaatacaat taaccacatc acccccggcc ttgcataatg caaatagact
     6001 attgcgccat cggccggggc ccttcgccaa ccggcaggat aggtgccagc gggaatcggt
     6061 tcggctcttg tctcagctcg cgaacagcag cttctcgcgc tggaacaggc gcaccgcctg
     6121 ccagaagatc agcgccgtca gcgcgagggt caccgccgcg cacagcgcga tctggccgag
     6181 cggcaccgcg agccccccga ccagctggtt gaactgggcg agctggccca tcagcggcac
     6241 cgacgcgacg ccgagcgtcg atcgcaaggg cgagaacacc atcaccatcc ccggtagcgc
     6301 cggcaccagc ggcagcagcc cgagatagat ctgcgcctcc ttcatcgacc gcgaggcgag
     6361 cgcgatggcg atctggaacg ccaccgccag cgccatcagc ggcaccgcga tcgcgaagat
     6421 ggtcaggaac gcgccg
//
LOCUS       DASWHU010000012         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_743012, whole genome shotgun sequence.
ACCESSION   DASWHU010000012 DASWHU010000000
VERSION     DASWHU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743012"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..424)
                     /locus_tag="VGB88_00440"
     CDS             complement(65..424)
                     /locus_tag="VGB88_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645932.1"
                     /translation="MPSPVVLTVGANRLDARLSPFFGLAPWVMVVEPLVAAPVWVRNT
                     GGNIAHVVDLIRAQAPALAICGHIPADAALLMVEAGIELRIGPCSVPATSLIPLAATL
                     PTPNIAPMRSTRGPGSA"
     gene            593..1360
                     /locus_tag="VGB88_00445"
     CDS             593..1360
                     /locus_tag="VGB88_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9645933.1"
                     /translation="MAKPNSRTVLKSQLASPCSACPVRDWALCQALPEGDLAVVERFM
                     TGHRTLSPGTDFYHQGEPSEELFTVLEGWGFQYRLLEDGRRQIVRVLLPGDFVGFQPD
                     LFAPVAHSAQALTEMRLCIFPRQNLLDLFRDQPQLALRLTWMLARDDSRAQDWLVNVG
                     RRSARERVAHLLLELYYRVRMRAEEPLGSSIPLPLTQEHIGDALGLTSVHVNRTLRDL
                     RQSGLLVVSNRVLNIVDPDGLAEVAGFDSETLQYLQP"
     gene            complement(1373..2218)
                     /locus_tag="VGB88_00450"
     CDS             complement(1373..2218)
                     /locus_tag="VGB88_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEX9645934.1"
                     /translation="MARERIELSRKKLRLDKVAMRRRFKSKDDYLARESQAQKDMLAL
                     QQAYFHQGLRGVIVFEGWDASGKGGAIRRLTARLDPRGTKVWPIGPPGADEQGRHYLY
                     RFWARLPTPGTIAVFDRSWYGRVLVERVDELVEAAAWQRAYDEINQFEHLLLADGVRL
                     AKLFLHITPDEQLRRFQERLHNPFKRWKLTADDLRNRAHWDAYKTAYEDMFDRTSTRE
                     APWTVIAGDHKWTARVQVLEEVTAQLGRGIDVAPPPLDPGVVAAAESALGLRIEPATD
                     TAPDS"
     gene            complement(2257..2541)
                     /locus_tag="VGB88_00455"
     CDS             complement(2257..2541)
                     /locus_tag="VGB88_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645935.1"
                     /translation="MAGTTTLCADQSAPRGWAVVAKSRNFDCGTNAYASWQWVDLRNS
                     APGRTAMVCLNPTIDRNLLPPGWVATGAGDTSIKCGPWGDNERIEVSYRP"
     gene            complement(2795..>3836)
                     /locus_tag="VGB88_00460"
     CDS             complement(2795..>3836)
                     /locus_tag="VGB88_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645936.1"
                     /translation="RHGRVIIEAYRSQEREVMDGAERNGMSAAALVRRGLADDGWGWS
                     LGRYGAIAEFARMPGDGAEVRLGPEGGEVAGRLGAIRITLTQDVVPLAYETLSRDPAR
                     WRHGLAFCLPAAAAQGEPETLTEIGPDGEAVCARDRDAVLFDMGVGSAAVRACVRTGD
                     AELIAILRASAGRSLLDPSHRAMAAILARSPHRVFASALGRIEVYQGIASERTPDGPH
                     THLLPRLLAAGRDHSANIPVPGGMVAPLIAYPPNPVTDSAGRPRPFDRAAHAAFQDML
                     AAWGAPAALAAKRNAEAAIARGAPPPVPGEPMARAERTARRVALRQLCCLRGPSPALD
                     AWRQAFEPARRA"
BASE COUNT          530 a         1406 c         1357 g          543 t
ORIGIN      
        1 gagaacggcc acgcgctcat ccccgaccgt ccgggcagcg gcatcgactg ggacgaggcc
       61 gcggtcaagc gctaccaggt cccctcgtag agcgcatcgg cgcgatgttc ggcgtcggca
      121 aggtcgcggc gagcgggatc aagctggtgg cgggcaccga gcacggcccg atgcgcagct
      181 cgataccggc ctcgaccatc agcagcgcgg catcggcggg gatatggccg cagatggcga
      241 gcgcaggggc ctgtgcccgg atcaggtcga cgacgtgcgc aatgttgccg cccgtattcc
      301 gcacccatac gggcgcggcc accagtggct cgaccaccat cacccacggc gccagaccga
      361 agaacggcga taggcgtgca tccagacggt tagcgccgac cgtcaacact accggcgacg
      421 gcatgggcgg tcagccccgc ccggcgaaca ggttgtggtt ggctcgcata acgaaatctg
      481 acgccaagcg cttccgtcgc gctctgatcg agattaaagt cgcgacctgg ccagggcgcc
      541 gaaggggcac ggcgtgcggg gtctaggggg cggagaggga gacttgacgg caatggcgaa
      601 gccaaacagc cgcaccgtcc tgaagtcgca gctggcgagc ccgtgcagcg cctgcccggt
      661 gcgcgattgg gcgctgtgcc aggcgctgcc cgaaggcgat ctcgcggtcg tcgagcggtt
      721 catgaccggc caccggacgt tgtctcccgg caccgacttc taccatcagg gcgagccgag
      781 cgaagagctg ttcaccgttc tcgagggctg gggcttccag taccgcctgc tcgaggacgg
      841 gcggcgccag atcgtccgcg tcctgctgcc cggcgacttc gtcggcttcc agcccgacct
      901 gttcgcgccg gtcgcgcaca gtgcccaggc gctgaccgag atgcggctgt gcatctttcc
      961 gcgccagaac ctcctcgacc tgttccgcga ccagccccag ctcgcgctgc gcctgacctg
     1021 gatgctggcg cgcgacgaca gccgcgccca ggactggctg gtcaacgtcg gccgccgctc
     1081 ggcgcgcgag cgcgtcgccc atttgctgct cgagctgtat taccgcgtgc gcatgcgtgc
     1141 cgaggagccg ctcggctcga gcatcccgct gccgctgacc caggagcaca tcggcgacgc
     1201 gctcggcctg acctcggtgc acgttaaccg gacgctgcgc gacctgcgcc agagcggcct
     1261 gctggtggtg tccaaccggg tgctcaacat cgtcgacccc gacggcctgg ccgaggtcgc
     1321 gggcttcgat tccgaaacgc tccagtatct gcagccgtaa ccgccgcgcg ccttacgaat
     1381 cgggcgccgt gtcggtcgcc ggctcgatcc ggaggccaag cgccgactcc gccgcggcca
     1441 ccacccccgg gtcgagcggc ggcggcgcca cgtcgatgcc gcggccgagc tgggccgtga
     1501 cctcctccag cacctgcacc cgggcggtcc acttgtggtc gccggcaatc accgtccacg
     1561 gcgcctcccg ggtcgaggtg cggtcgaaca tgtcctcgta cgccgtcttg taggcgtccc
     1621 aatgcgcccg gttgcgcaag tcgtcggcgg tcagcttcca acgcttgaac gggttgtgca
     1681 gccgctcctg gaaccggcgc agctgctcgt cgggcgtgat gtgcaggaac agcttcgcca
     1741 gccgcacgcc gtcggcgagc agcaggtgct cgaactggtt gatctcgtca taggcgcgct
     1801 gccaggcggc ggcctcgacc agctcgtcga cgcgctcgac gaggacccgg ccgtaccacg
     1861 agcggtcgaa cacggcgatg gtgccgggcg tcggcaggcg cgcccagaag cggtacaggt
     1921 agtggcgccc ctgctcgtcg gcgcccggcg ggccgatcgg ccacaccttg gtgccgcgcg
     1981 ggtcgaggcg cgcggtcagc cggcggatcg cgccgccctt gcccgacgcg tcccagccct
     2041 cgaacacgat caccccgcgc aggccctggt ggaaatatgc ctgctggagc gcgagcatgt
     2101 ccttctgcgc ttggctctcg cgcgccagat agtcgtcctt cgacttgaac cggcggcgca
     2161 tcgccacctt gtccagtcga agcttcttgc gcgagagctc gatccgctct cgggccatgg
     2221 cgaggtcgcg ggccgatcag taagccggcg cgccggtcac ggccggtagc tgacctcgat
     2281 gcgctcgttg tcgccccacg ggccgcactt gatcgacgtg tcgcccgcgc ccgtcgccac
     2341 ccatccgggc ggcagcaggt tgcggtcgat ggtcgggttg aggcagacca tcgccgtccg
     2401 ccccggcgcg gagttgcgca ggtcgaccca ttgccagctg gcataggcgt tggtgccgca
     2461 gtcgaaattg cggctcttgg ccaccaccgc ccagccgcgc ggcgcgctct ggtcggcgca
     2521 cagcgtggtc gtcccggcca tctgtgcgcc gggcgccatt tgcgcgcagc cgccgacggt
     2581 cgcgacgcat aggcccgcaa ccagcagtgc ccgtctcgcg ccgtccctgg cgcgtgccgg
     2641 aagctcggaa ctcatggcgt gcccccattt gcttatgatt tgccgtgacg tgccgtgcgc
     2701 acgcaggtgg cccggacatc tcggttgccg catcttaggc gaaagcccga ccggcgagaa
     2761 acccaacaat tgcggccccg gcggcggcgg ctcgtcaggc gcgccgggcg ggctcgaacg
     2821 cctgccgcca ggcgtcgagc gccgggcttg ggccgcgcag gcagcagagc tggcggagcg
     2881 cgaccctgcg cgcggtgcgc tcggcgcgcg ccatcggctc gccgggcacg ggcggcggcg
     2941 cgccgcgcgc gatcgcggcc tcggcgttgc gcttggcggc gagcgcggcc ggcgctcccc
     3001 atgccgccag catgtcctgg aacgcggcgt gggcggcgcg gtcgaacggc cgcggccggc
     3061 cggccgaatc ggtcaccggg ttgggcgggt aggcgatcag cggcgcgacc atgccgcccg
     3121 gcaccgggat gttggccgag tggtcgcgcc cggccgcgag cagcctcggc agcaggtggg
     3181 tatgcggccc gtcgggggtg cgctcggagg cgattccctg gtagacctcg atccgaccga
     3241 gggccgaggc gaacacccgg tgcgggctgc gggcgaggat cgcggccatc gcccggtggc
     3301 tggggtcgag cagcgaccgc ccggccgagg cgcgcaggat cgcgatcagc tccgcatcgc
     3361 cggtgcgcac gcaggcgcgg accgcggccg agccgacccc catgtcgaac aacactgcgt
     3421 cccggtcgcg cgcgcacacc gcctcgccgt cggggccgat ctcggtcagc gtctccggct
     3481 cgccctgcgc tgcggccgcg ggcaggcaga aggcaagccc gtgccgccag cgcgccgggt
     3541 cgcgcgacag cgtctcgtag gcgagcggca ccacgtcctg ggtgagcgtg atccggatcg
     3601 cccccaggcg gcccgccacc tcgccgccct caggcccgag ccggacctcg gcgccgtcgc
     3661 cgggcatgcg cgcgaactcg gcgatcgccc catagcggcc gaggctccag ccccagccgt
     3721 catcggcgag cccgcgccgg accagcgccg ccgccgacat cccgttccgc tccgcgccgt
     3781 ccatgacctc ccgctcctgg ctgcgatacg cttcgattat aacgcggcca tgccga
//
LOCUS       DASWHU010000013         8218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2960965, whole genome shotgun sequence.
ACCESSION   DASWHU010000013 DASWHU010000000
VERSION     DASWHU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2960965"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGB88_00465"
     CDS             <1..496
                     /locus_tag="VGB88_00465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970305.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX9645937.1"
                     /translation="GGASPGIEPNAANSFTHKTLSGSFHVRNRYLERLLEERGRNDAD
                     VWSSIIAHEGSVQHLDFLDDDDKATFRTAFELDQRWLVELAADRAPYICQSQSVNLFL
                     PANVHKRDLHRIHFLAWKKGVKSLYYCRSKSLQRADVVASPHVVAAMTEQPAAEAEEC
                     LACQ"
     gene            526..780
                     /locus_tag="VGB88_00470"
     CDS             526..780
                     /locus_tag="VGB88_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645938.1"
                     /translation="MLMRHRVWRIVEKICVGALIVLIVQSEGGTIALIGKEGGTFRIV
                     HPAVAEIESLPASRLSYQHPIRVEEVTVSTYASSTSSFKA"
     gene            780..1739
                     /locus_tag="VGB88_00475"
     CDS             780..1739
                     /locus_tag="VGB88_00475"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX9645939.1"
                     /translation="MSLLDASPVYKPFHYPWAYDAWLIQQRIHWLPEEVPLADDIKDW
                     HRHLAPEERNLLTQIFRFFTQSDVEVNTCYMRHYGRVFQPTEVQMMLAAFSNMETVHI
                     AAYSHLLDTIGMPESEYSAFLHYKEMKDKYDFMRQFGTATKADIATTLATFGAFTEGL
                     QLFASFAVLLNFPRFNKMKGMGQIVSWSVRDETLHTHSMIRLFHTLIEENPEIWTDAL
                     SRNLYRICASVVDHEDAFIDRAFELGPVEGLGPDDVKSYIRYIADRRLMQLHLQPIYR
                     VARNPLPWLEHLLNGAEHTNFFENRATEYSRAATQGTWEEAFD"
     gene            1886..3001
                     /locus_tag="VGB88_00480"
     CDS             1886..3001
                     /locus_tag="VGB88_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9645940.1"
                     /translation="MQPMQGIRVLEFGDGVAVAMTGLILAEAGAEVIKVESPRSGAAA
                     RAGEPRLGAVALDFALLNAGKKSVAVDLDADGAAARLRPLLASADVLIDGLRPGTMGE
                     RGLDYDAVAAVNDRLLYCSLSGWGQDGPLAREAGRDLNYLGATGLLRLGADETGAPVL
                     PPAEIADIAAGAYPAVINILLALMARAQSGKGARLDLAICDGLFALAYWAIGRAELTG
                     RWPVPGGETLTGGSPRYQLYATADGRFVAVAALEPDHWDNLCERLELDEELRDDQRDP
                     EATRQALAAIIAARTARYWHDKFENRDLCCSVVETLEQALANPQFRDRGLFERKLRAG
                     GQAITAVVVPVAASTRESDRVRAAPALGADNALLDQA"
     gene            3185..3427
                     /locus_tag="VGB88_00485"
     CDS             3185..3427
                     /locus_tag="VGB88_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645941.1"
                     /translation="MKVIFPAAVLACLLAAGTAVADEASSATSLQVIGPHQTRELATQ
                     TAGQVSAAAAEATRQAQAESAQAGDADSTTHTASTR"
     gene            3793..4758
                     /locus_tag="VGB88_00490"
     CDS             3793..4758
                     /locus_tag="VGB88_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX9645942.1"
                     /translation="MGPHQLRVLGGMVANGIANRVGEATRGTGLVERAAGEEPSTVAG
                     SEPGTGLSAGEQMTGMSAGSNAGRLGIWVSGGWTQYEDDLASTAYHGDAYNVLAGGDY
                     QFNDRFIAGLALGYESSDVDTIFNNGNLNSDGFTIAPYAGYVLNRYFSIDVSGGYSWI
                     DYGQRRTAAGGTVSGENDSNRWFGSANLNGFYAVDRVVLSGKVGYLYTEEEQEGYVES
                     DGTVNTANTVDLGQLRAGGQVGYDLGKVQPYVTGSYVYDIQHELLKVGPGQAQPANDR
                     SGFDVGGGIRFALSDRVSGGIQGTKHLGRENFDSTAVNGNLRIKF"
     gene            4814..5644
                     /locus_tag="VGB88_00495"
     CDS             4814..5644
                     /locus_tag="VGB88_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9645943.1"
                     /translation="MACASLAALAAALGACSTGLLEDGRDRAIRIATDNGFTPAHVDG
                     GQFTLFSASRARPGGGDRLVVYVEGDGRAWANRYVLASDPTPLEPMALKLAARDPAPL
                     VAYLARPCQYTRAEADRACPSRYWSGHRYAPEVIAATGRVIDHYKSLLGAREVELVGY
                     SGGGAVAALLAAERDDVVALTTVAAPLDHRAWTAHHGLSPMAGSLNPADFAAALAKVP
                     QVHLVGTDDSVVPPAVAEAYLAHLGDRSHSRLVRIEDFGHTCCWERRWPELRAAYHAS
                     "
     gene            complement(5667..6416)
                     /locus_tag="VGB88_00500"
     CDS             complement(5667..6416)
                     /locus_tag="VGB88_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645944.1"
                     /translation="MPLLPRRPAHPSEIEVQPLHRLARVPVHLTRRAARLALLALVAA
                     DLALVGAFLSTGSPEYPRPASLELGGPATIPWSFDVLKLWAAAALAALMALSYPYDRR
                     ADPFWWLAAAVAALLGLADMAGLHRVWVAAVAPAVLGGPVDARLELVSQGAPLAVLYL
                     AAAARLPAWSRAGTGAVAASAAAFVAAWLGTPEVLAAPWTAAVGADAVAAAWHHGLIG
                     LSRSLLLGGLMMTVAEVQARAVRYVYSGDAP"
     gene            complement(6425..7420)
                     /gene="pip"
                     /locus_tag="VGB88_00505"
     CDS             complement(6425..7420)
                     /gene="pip"
                     /locus_tag="VGB88_00505"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX9645945.1"
                     /translation="MTGAGELHRAVEPFDRRRLEVDSRHSLYLEQFGRPDGVPAVMLH
                     GGPGSGCQRDHARLFDPARFRAVLFDQRGAGMSTPALSLVDNTTQHLVADIERLRDAL
                     GIERWLVVGGSWGSTLAVAYAEAHPDRVLGLVLRAVFLGSRDETEWAFAGAARRFWPE
                     LWRRFVGLLSEAERADPVAAYGARLEHAEARIHVPAARAWGAYERALSELRPGAAGLP
                     ESLDDPTATAGARAPRSPYVEWHYIKHDFFLEPGQLLADARRLAAIPGVIAQGRYDLL
                     CPPVTAARLAAAWGNVELRIAEASGHAASEPGLRAALLEAVAAFGDRFAGTERVR"
     gene            complement(7417..>8218)
                     /locus_tag="VGB88_00510"
     CDS             complement(7417..>8218)
                     /locus_tag="VGB88_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9645946.1"
                     /translation="AAPAHPVPLPKPAQGAIAFQAVTFFYPSRPDAASLTELTLDIAP
                     GERVALVGPSGAGKTTLFQLLLRFYDPHAGRITFDGIDLCDLAPAELRARLALVPQEP
                     VLFAADAWENIRYGRPEAGDDEVRAAAAAAAADGFLDALPDGFATFLGERGVRLSGGQ
                     RQRIAIARAVLRDPAVLLLDEATSALDSESERLIQQALERLMAGRTTLVIAHRLATVQ
                     NVDRIVVLDRGRIVASGSHHRLMAEGGLYARLAELQFADSAPRLRASS"
BASE COUNT         1237 a         2923 c         2918 g         1140 t
ORIGIN      
        1 cggcggcgcc tcgcccggca tcgagccgaa cgcggccaac agcttcaccc acaagacgct
       61 gtcgggctcg ttccacgtcc gcaaccgcta tctcgagcgc ctgctcgagg agcgcgggcg
      121 caacgacgcc gacgtgtggt cgtcgatcat cgcccacgag ggctcggtgc agcacctcga
      181 cttcctcgac gacgacgaca aggcgacctt caggaccgcg ttcgagctcg accagcgctg
      241 gctggtcgag ctggcggccg accgcgcgcc ttacatttgc cagtcgcagt cggtcaacct
      301 gttcctgccc gccaacgtcc acaagcgcga cctgcaccgc atccacttcc tggcctggaa
      361 gaagggggtg aagagcctct actactgccg ctcgaagtcg ctgcagcgcg ccgacgtggt
      421 ggcgagtccg cacgtcgtgg ccgcgatgac ggagcagcca gccgccgagg cagaggagtg
      481 cctggcgtgc caataagaat tattgttgac gcggcgagtc attcgatgct catgcggcat
      541 cgtgtttggc gtatcgtcga aaaaatttgt gtaggggcat tgattgtcct catcgtgcag
      601 tcggaaggtg gcacaattgc tttgatcggg aaggaaggcg gcacattccg aattgttcat
      661 cctgccgttg ccgaaatcga gtcgttgccc gcttcgagac tgagttatca gcatccaatc
      721 agggtcgaag aagtgaccgt ttctacttat gcgtcttcaa cttcatcgtt taaggcgtga
      781 tgtcgctgtt ggacgcgagc ccggtctaca agccgttcca ctacccgtgg gcatacgacg
      841 cctggctcat ccagcagcgc atccactggc tgcccgagga ggtgccgctg gccgacgaca
      901 tcaaggactg gcaccgccac ctggcgcccg aggaacgcaa cctgctgacc cagatcttcc
      961 gcttcttcac ccagtcggac gtcgaggtca acacctgcta catgaggcac tacgggcggg
     1021 tgttccagcc gaccgaggtg cagatgatgc tggccgcgtt ctccaacatg gagaccgtgc
     1081 acatcgccgc ctactcgcat ctcctcgaca ccatcggcat gcccgagagc gagtactcgg
     1141 ccttcctcca ctacaaggag atgaaggaca agtacgactt catgcgccag ttcggcaccg
     1201 cgaccaaggc cgacatcgcc accacgctcg ccaccttcgg cgccttcacc gagggcctgc
     1261 agctgttcgc gagcttcgcg gtgctgctca acttcccgcg cttcaacaag atgaagggga
     1321 tgggccagat cgtctcctgg tcggtgcgcg acgagaccct gcacacgcac tcgatgatcc
     1381 gcctgttcca caccctgatc gaggagaacc cggagatttg gaccgacgcg ctgagccgca
     1441 acctgtaccg gatctgcgcc agcgtggtcg accacgagga cgcgttcatc gaccgcgcct
     1501 tcgagctcgg cccggtcgag ggactgggcc cggacgacgt caagagctac atccgctaca
     1561 tcgccgaccg gcgcctgatg cagcttcacc tgcagccgat ctatcgcgtg gcgcgcaatc
     1621 cgttgccgtg gctcgagcac ctgctcaatg gcgccgagca caccaacttc ttcgagaacc
     1681 gcgccaccga atactctcgc gccgctaccc aaggtacctg ggaagaggcg tttgactagc
     1741 cgcgtcgccc gctaccctcc cggcgggcgc gcggcctttg ttttcctccc tgatccgatc
     1801 ggccgcgccg gttgcggcgg agaacgcttg agtaccggcc gggggccctc ataatcccgg
     1861 gacgttggcg gagcaggagt ggcgcatgca gccgatgcag ggaatccggg tgctcgagtt
     1921 cggcgacggc gtggcggtgg cgatgaccgg cctgatcctc gccgaggccg gcgccgaggt
     1981 gatcaaggtc gaaagcccgc gctcgggcgc cgcggcgcgc gccggcgagc cccgcctcgg
     2041 cgcggtcgcc ctcgacttcg cgctgctcaa tgccggcaag aagagcgtcg cggtcgacct
     2101 cgacgcggac ggcgccgcgg cccgcctgcg cccgttgctc gccagcgccg atgtcctgat
     2161 cgacgggctg aggcccggga ccatgggcga gcgcgggctc gactacgacg cggtcgcggc
     2221 ggtcaacgac cggctgctgt actgctcgct gagcggctgg ggacaggacg gcccgcttgc
     2281 ccgggaggcc gggcgcgatc tcaactatct cggcgccacc ggcctgctgc gcctcggcgc
     2341 cgacgagacc ggcgccccgg tgctgccccc ggccgagatc gccgacatcg ccgccggcgc
     2401 ctatccggcg gtgatcaaca tcctgctcgc gctgatggcg cgcgcgcagt cgggcaaggg
     2461 cgcgcggctc gacctcgcga tctgcgacgg gctgttcgcg ctcgcctact gggcgatcgg
     2521 gcgcgccgag ctgaccgggc gctggccggt gccgggcggc gagaccctga ccggaggctc
     2581 gccgcgctat cagctctatg ccaccgccga cggcaggttc gtcgccgtcg ccgcgctcga
     2641 gccggaccat tgggacaatc tgtgcgagcg gctcgagctc gacgaggagc tgcgcgacga
     2701 ccagcgcgac ccggaagcga cccgccaggc gctggcagcg atcatcgccg cacgcaccgc
     2761 caggtactgg cacgacaagt tcgagaaccg ggacctgtgc tgctcggtgg tcgaaacctt
     2821 ggagcaggcg ctggccaatc cccagttccg cgaccgcggc ctgttcgagc gcaagctgcg
     2881 cgcgggcggc caggcgatca cggccgtggt ggtgccggtg gcggcgagca cccgggaatc
     2941 ggatcgcgtc cgcgcggcgc ccgcgctcgg cgccgacaat gcgctgctcg accaggcgtg
     3001 atctcgcggc cttactcgac cgttcggtcc aaaagcccgc ccgcgcggtg gccgcgaccg
     3061 tcggcaaggc ctgtgtggcg gccccggccg ggcgtgccga tttgactcgt ccggccggcg
     3121 gcaaggggcc atattataag gattaacgct ccgatcgtta cttgatccgg ctcaggagga
     3181 attcatgaaa gtcattttcc cggcagccgt tctcgcctgc ctgctcgccg ccggcacggc
     3241 ggtcgccgac gaggcgtcga gcgccacctc gctccaggtc atcgggccgc accagacccg
     3301 tgagctcgcc acccagaccg ccggccaggt ctcggccgcg gcggccgagg ccacccgcca
     3361 ggcccaggcg gagtcggcgc aagccggcga cgcggactcc accacccata cggcaagcac
     3421 ccgataggcc gtccgagccc gaccgggcgc gcagccgccg cgccgggggc gaccgcgctc
     3481 cgtttgccgc gctgttgtag aactgcaaca ggggcctcga atttcccgcc gggacggcgc
     3541 tcaattcctg gcaagtgttt gactgcactg gtcttgatcc tgtagcagat tacctcagct
     3601 ttacggtcgc cggccggcgg ggcgaggctg gttgtacgcc gcggtttgac gcccgagtga
     3661 caggggtagt cgatgtcgaa ttttcgcgcg attgggcttg tcgcggccgc ggtgttggcc
     3721 gcgtcgccgg catgggcgac gtccgatacg gaggacaagt cccaaaccac ctttcagcag
     3781 ggcgaaaagg tcatggggcc gcaccagctc cgggtgctcg gcggcatggt cgcgaacggg
     3841 atcgccaacc gggtaggcga ggccactcgc gggaccggct tggtcgagcg ggcggccggc
     3901 gaggagccct cgaccgtggc cggctccgag cccggcaccg gcctgtcggc cggcgagcag
     3961 atgaccggca tgtcggccgg cagcaatgcc gggcgtctcg gcatctgggt gtcgggcggc
     4021 tggacgcagt acgaggacga cctggcgtcg accgcctacc acggcgacgc ctacaacgtg
     4081 ctcgccggcg gcgattacca gttcaacgac cggttcatcg ccggtcttgc gctcggttac
     4141 gagtcgagcg atgtcgacac gatcttcaac aacggcaacc tgaacagcga cggcttcacc
     4201 atcgcgccct atgccggcta cgtgctgaac cgctacttct cgatcgacgt cagcggcggc
     4261 tacagctgga tcgactacgg gcagcggcgc actgcggccg gcggcacggt gtccggcgag
     4321 aacgattcca accgctggtt cgggtcagcc aacctcaacg gcttctacgc cgtcgaccgg
     4381 gtcgtcctca gcggcaaggt cggctacctg tacacggagg aggagcagga gggctacgtc
     4441 gagagcgacg gcaccgtgaa cacggcaaac accgtcgatt tgggccagct ccgggccggc
     4501 ggccaggtcg gttacgatct cggcaaggtt cagccgtacg tcaccggcag ctacgtctac
     4561 gacattcagc acgagctgct caaggtcggc ccgggtcagg ctcagccggc gaacgaccgc
     4621 tcgggcttcg atgtcggcgg cggcatccgg ttcgcgctca gcgaccgcgt cagcggcggc
     4681 atccagggca ccaagcatct gggccgcgag aatttcgact cgaccgcggt gaacggcaac
     4741 ctgcgcatca agttctgatc cgcgggtgga cgcgcgcgca atcgaagggg cccggcggcg
     4801 atgccgggcc cctttggctt gtgcctcgct cgccgccctg gccgcggcgc tcggcgcctg
     4861 ctctaccggg ctccttgagg acggccgcga ccgggccatc cgcatcgcca ccgacaacgg
     4921 tttcacgccg gcccatgtcg acggcggcca gttcacgctg ttttccgcct cgcgcgcccg
     4981 gcccggcggc ggcgatcggc tggtggtcta tgtcgagggc gacgggcggg cgtgggcgaa
     5041 tcgctacgtg ctggcgtccg acccgacccc gctcgagccg atggcgctca agctggcggc
     5101 gcgcgacccc gcgccgctgg tcgcctacct ggcgcggccg tgccagtaca cccgcgccga
     5161 ggccgaccgg gcatgcccgt cgcgctactg gtcgggccac cgctacgcgc ccgaggtgat
     5221 cgcggcgacc gggcgggtca tcgaccacta caagtcgctg ctcggggcgc gcgaggtcga
     5281 gctggtcggc tactcgggcg gcggcgcggt cgccgcgctg ctcgccgccg agcgcgacga
     5341 cgtggtcgcg ctgaccacgg tggcggcacc gctcgaccat cgggcgtgga cggcgcatca
     5401 cggcctgagc ccgatggccg gctcgctcaa cccggccgac ttcgccgccg cgctcgccaa
     5461 ggtgcctcag gtgcacctgg tcggcaccga cgactcggtg gtgccgccgg cggtcgccga
     5521 ggcctacctg gctcacctgg gcgaccgctc gcacagccgc ctggtgcgca tcgaggactt
     5581 cggccacacc tgctgctggg agcgccgctg gcccgagctg cgcgccgcct accacgcctc
     5641 gtgagggtgc ggccggcggg cgccgctcac ggcgcgtcgc cgctatagac gtagcgcacg
     5701 gcgcgcgcct ggacctcggc cacggtcatc atcaggccgc cgaggagcag gctgcgcgac
     5761 agcccgatca ggccgtggtg ccaggcggcg gcgaccgcat cggcgccgac cgccgcggtc
     5821 cagggcgcgg ccaggacctc aggcgtgccc agccacgcgg ccacgaacgc cgccgcggag
     5881 gcggcgaccg cgcccgtgcc cgcccgcgac cacgccggca ggcgcgccgc cgcggccagg
     5941 tacagcaccg ccagcggcgc gccctggctg accagctcga ggcgcgcatc gaccgggccg
     6001 ccgagcaccg ccggggccac ggccgcgacc cagacccggt gcagccccgc catgtcggcg
     6061 aggccgagca gcgcggcgac ggccgcggcc agccaccaga acgggtccgc ccggcggtcg
     6121 taggggtagc tcagcgccat cagggccgcg agcgccgccg ccgcccacag cttgagcacg
     6181 tcgaagctcc aggggatggt cgccggcccg ccgagctcga ggctcgccgg ccgcgggtac
     6241 tcgggcgacc cggtcgaaag aaaggcgccg accaacgcca ggtcggcagc gaccagggcc
     6301 agcagcgcga ggcgagcggc gcgacgggtg aggtggaccg gcacccgcgc cagccggtgc
     6361 agcggctgga cctcgatctc gctggggtgc gccgggcggc gcggcaaaag cggcatggcg
     6421 cgtgctatcg gacgcgctcg gtgccggcga agcggtcgcc gaaggcggcg acggcctcga
     6481 gcagcgccgc gcgcaggccg ggctccgagg cggcgtggcc gctggcctcg gcgatgcgca
     6541 gctcgacgtt gccccaggcg gcggcgagcc gcgccgcggt caccggcggg cacaacaggt
     6601 cgtagcggcc ctgggcgatg acgcccggta tcgccgccag ccgccgggcg tcagcgagca
     6661 gctggccggg ctcgagaaaa aagtcgtgct tgatgtagtg ccactcgaca taggggctgc
     6721 gcggcgccct cgcgccggcc gtggcggtgg ggtcgtcgag gctctccggc aggccggcgg
     6781 cgcccgggcg gagctcggac agcgcccgct cgtaggcgcc ccaggcgcgc gccgccggga
     6841 cgtggattcg cgcctccgcg tgctcgaggc gggcgccgta ggcggccacc gggtcggcgc
     6901 gctcggcctc ggacaggagc ccgacgaagc gccgccacag ctcgggccag aaccgccgcg
     6961 ccgcgccggc gaatgcccac tcggtctcgt cgcggctgcc gaggaagacc gcgcgcagca
     7021 ccagccccag cacccggtcg ggatgggcct cggcataggc gacggcaagc gtcgagcccc
     7081 acgagccgcc gaccaccagc cagcgctcga taccgagcgc gtcgcgcagc cgctcgatgt
     7141 cggcgaccag gtgctgggtg gtgttgtcga ccagcgacag cgcgggcgtg ctcatgccgg
     7201 cgccgcgctg gtcgaacagc acggcgcgga accgcgccgg gtcgaacagg cgcgcatggt
     7261 cgcgctggca gccgctgccc gggccgccgt gcagcatcac cgccggcacg ccgtcggggc
     7321 ggccgaactg ctcgagatag aggctgtgcc gcgaatcgac ctcgagccgg cggcggtcga
     7381 acggctccac cgcgcggtgc agctcgcccg ccccggtcat gagctcgccc gcaggcgcgg
     7441 cgcgctgtcc gcgaactgca gctcggcgag gcgcgcgtag agcccgccct cggccatcag
     7501 ccggtggtgg ctgcctgagg cgacgatgcg gccgcggtcg aggaccacga tgcggtcgac
     7561 gttctggacc gtggccaggc ggtgggcgat caccaaggtg gtgcgcccgg ccatcagccg
     7621 ctcgagcgcc tgctggatca gccgctcgct ttcggaatcg agcgcgctgg tcgcctcgtc
     7681 gagcaacagc accgcgggat cgcgcagcac cgcgcgcgcg atcgcgatgc gctggcgctg
     7741 gccgcccgac aggcgcacgc cgcgctcgcc gaggaaagtc gcgaagccgt cgggaagtgc
     7801 gtcgaggaag ccgtcggcgg cggcggctgc ggcggcggcg cggacctcgt cgtcgccggc
     7861 ctcggggcgg ccgtagcgga tattctccca ggcatcggcg gcgaacagca ccggctcctg
     7921 cggcaccagc gcaaggcggg cgcgcagctc cgccggcgcg aggtcgcaca ggtcgatgcc
     7981 gtcgaaggtg atgcgtccgg cgtgcgggtc gtagaacctg agcaggagct ggaacaaggt
     8041 ggtcttgccg gcgccggacg ggccgaccag ggcgacgcgc tcgccgggcg cgatgtcgag
     8101 cgtgagctcc gtgagcgagg cggcgtcggg gcggctcggg tagaagaacg tcaccgcctg
     8161 aaaggcgatc gcgccctgtg ccggcttggg cagcggcacc ggatgcgccg gcgccgcg
//
LOCUS       DASWHU010000014        10034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_743218, whole genome shotgun sequence.
ACCESSION   DASWHU010000014 DASWHU010000000
VERSION     DASWHU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743218"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGB88_00515"
     CDS             <1..343
                     /locus_tag="VGB88_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9645947.1"
                     /translation="REITPDEVLLFRYSALTFNGHRIHYDYPYVTGEEGYEGLIVHGP
                     LTATLLLDLVGREAPEARIAGVRFRAHHPLYAGRKLTLEAAPESGAIAAWALDSAGRL
                     AMHARIETTGH"
     gene            365..1375
                     /locus_tag="VGB88_00520"
     CDS             365..1375
                     /locus_tag="VGB88_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9645948.1"
                     /translation="MEYRRLGTSGVTVSRLCLGTMMFGDQADETAAKRIADMAWDAGI
                     NFIDTADVYAGGASEEVTGKVIRRRRDRWVLATKLGQAARANDANAGGLSRRWITAEL
                     EASLKRLGTDHVDILYFHREDLACALEESLRAVGDAIRAGKVRYLGLSNFRAWRHAEV
                     ARLCDSLGMDRPVASQPYYNAFNRMPETEVLKACQHYGMGVVPYSPIARGVLTGKYPP
                     GKAPARGTRAGRKDRRMMQTEFREESLVLAQRMVDYAKRRGMTPVLFAVRWVLENPIV
                     TATIAGPRTPEQMQGYLGCFDHDWTADDEAFVDSLVAPGHPSTPGYNDPGYVIEGRPA
                     RV"
     gene            complement(1545..2297)
                     /locus_tag="VGB88_00525"
     CDS             complement(1545..2297)
                     /locus_tag="VGB88_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004547249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9645949.1"
                     /translation="MQYLFDLAASPGAWAALATLTAMEVVLGIDNLIFISILTNRLPE
                     HQRALGRRVGIGLALILRLALLGSVAYIARLTEPIVTLFDHAFSWRDLIMLAGGVFLL
                     WKATIEIHHRVDPTVEPSAAAQAAAGFAVTVAQILVFDLVFSIDSILTAVGMTTHFPI
                     MVVAVLIAVAVMMFAATPVADFIHANPTVVMLALGFLMMIGMLLLADGFGVHVPKGYV
                     YAAMAFSALVEALNMLARRARQARARGTAAAP"
     gene            complement(2525..4321)
                     /locus_tag="VGB88_00530"
     CDS             complement(2525..4321)
                     /locus_tag="VGB88_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9645950.1"
                     /translation="MATGYAAPLADMRFVLADMIGLEALAALPGLDQVTADLVDQVLG
                     EAGRFAADVLAPLNQPGDAQGAEFVNGVVRMPAGFAEAYARWVEAGWGAAGADPAHGG
                     MGLGMTVTTALNEMWQSANMALADGLMLTQGAVDAIESHGSDEQKRLYLPKLVSGAWS
                     GTMNLTEPNAGSDVGALRTRAVRTNGEWRITGSKIFITHGDQDMTENVVHLVLARTPG
                     SPPGTRGISLFLVPKVLVNDDGSLGARNDLRVVSIEHKMGHHASPTCVMSYGDDEGAL
                     ATLIGPERHGMQCMFTMMNNARLNVGVQGLAIAERACQAARDWARQRIQGAMIGERSG
                     EPVAIIRHPDVRRMLMDMRSQTEAMRLLCYSVAAALDRAKREPDEAARARAQGYADLM
                     TPVAKGWCTDQGFEVASLGVQVHGGMGYIEETGAAQHLRDARITMIYEGTNGIQALDL
                     VRRKLQQDGGEAVVRLIEDMRATAERLSQADHDGLRAIAAELAPAVDAFAHTTEWMQT
                     TFRDDPVAAAAGATPYMRMMGFTAGGWLMAEAAAKATEHLEHFTADEAFYTAKVDTAR
                     YFAAQYLAPAAALARPIMCASETVMALPEDAL"
     gene            complement(4371..5801)
                     /locus_tag="VGB88_00535"
     CDS             complement(4371..5801)
                     /locus_tag="VGB88_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9645951.1"
                     /translation="MTALAIKDLNDLAGRLRAIVGDKGLVTDAEDIAPHLLDQRGKYR
                     GKAAALVRPASTEEVAAVVRLCADLGVAVVPQGGNTSMCGGSVPHGHGREIIVNLSRM
                     NAIRDIDRLNYTMCAEAGCILADLQAAAAAAGRMLALSLAAEGSCEIGGNLSTNAGGT
                     NVLRYGMAREQVLGLEVVLADGRVWDGLRSLRKDNTGYDLKQLFVGAEGTLGIITAAV
                     LKLMPKPRSVLTAMVAVRDPDAAIELLAQAREETGDSVTGFELMARICLDFVLAHMPA
                     AQDPLADRHDWYVLAELSSPAADAGLEAAMERLLERALADGLVLDGTIAASEAQARNL
                     WALREHVSEAQKPEGGSIKHDISVPVSKVPELIRRAVALTERTIPGSRPVPFGHVGDG
                     NVHFNVSQPPGADREDFLARWDELNEVIHDLVAELGGSFSAEHGIGQLKRAALVKYRS
                     VVEVALMRTLKAALDPQGIMNPGKVV"
     gene            complement(5878..6840)
                     /locus_tag="VGB88_00540"
     CDS             complement(5878..6840)
                     /locus_tag="VGB88_00540"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9645952.1"
                     /translation="MSAARVVPASAQAIARAAEILRSGGLVAFPTETVYGLGADATSE
                     HAVARIFAAKARPSFNPLIIHLRDTEAAARHAKLDARARALARRFWPGPLTLVLAKRD
                     DSVLSGLALCGLRTVALRVPGHPVAHDLLAAAGRPIAAPSANRSGQLSPTTAGHVADG
                     LGDAVDLILDGGPTPVGIESTILDLTVAPPALLRPGAVTRAEIEAEIGRVAVGTEGAQ
                     PKAPGQLSRHYAPRTPVRLDATSVRPDEALLVFGPGAPPGARTTLNLSAAGDVTEAAA
                     NLFAMLHALDEAGAAAIAVMPIPETGLGAAINDRLRRAAETAAP"
     gene            complement(6837..8060)
                     /locus_tag="VGB88_00545"
     CDS             complement(6837..8060)
                     /locus_tag="VGB88_00545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9645953.1"
                     /translation="MRAAKGSADAAGAPRDAALPIRREVAELQPSGIRAIAHAGMGQD
                     GVIPLWFGEPDLPTPAFIVAASDAALSAGHTFYSHNRGVPELRETLADYTSRLYRKAI
                     GIERITVTAAGMNALMQVAELLVDPGDNIVIATPLWPNFFRCIEIMGGEARQVPMTIA
                     GDPRVQPGSWRLDLDRLFDACDARTRAITINSPNNPTGWMMDSETQAAVLDFCRDRGL
                     WLIADEVYARIVYDRPVAPSFLDHASDDDLVLVVNSFSKSWAMSGWRLGWITHPPALG
                     EAFEKLSEYNVASPGTGTQYAGVVAVRDGEPFVAEMVERYRIARDMVQQRLGAMRRVR
                     LSRPEAAFYGFFAVDGIDDSMAFAMQVLAETKVGLAPGIAFGAGGETYLRICYAKTAA
                     LLGEALDRLEPLLDR"
     gene            complement(8060..8551)
                     /locus_tag="VGB88_00550"
     CDS             complement(8060..8551)
                     /locus_tag="VGB88_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645954.1"
                     /translation="MIGRTSGSDTPTPDLRAKATEGGVRAGGIDPAAVEAARAVVRAR
                     GARYLDSLFADIAALEAELGVVAARGSAAALAAIARRARAMRAHGTTYGYGLVTRLAG
                     SLYDFCGSATGREDEIEVIEMHIQALKAVAGGRMTGDGALAGRDLVHAVEEAVSATAG
                     ARG"
     gene            complement(8548..9060)
                     /locus_tag="VGB88_00555"
     CDS             complement(8548..9060)
                     /locus_tag="VGB88_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9645955.1"
                     /translation="MAGAYWRLDRLSVLVVDDDPEITALLCDLFAALGFARTVAAPNG
                     EVARTMLADRKAQPIDLVVADVAVAPDRRATLTDWIRRHPRSPNRYLPVIVLADATDL
                     EAALAVRARGATEVLVKPVTVDSLCAVLEAIIEAPRQFVDTRSYFGPDRRRHEMPYRG
                     GERRDEAGAP"
     gene            complement(9084..>10034)
                     /locus_tag="VGB88_00560"
     CDS             complement(9084..>10034)
                     /locus_tag="VGB88_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX9645956.1"
                     /translation="ANFAGILAIGLGCEVNQIASMVERYGWQTGKMLSTMTIQDTGGT
                     RKTVAEGVARIKEMLPEANRVSRTTVPASHLILGLECGGSDAYSGITANPALGAAADL
                     LVRHGGTAVLAETPEIYGAEHLLTRRAVSKAVADKLIKHILWWEEYTAKNGAEMNNNP
                     SPGNKAGGLTTILEKSLGAVAKGGTTNLVDVYQYAEPVTAKGFCFMNTPGYDVTSVNG
                     MVAGGANVVCFTTGRGSAFGCKPTPSIKLATNTPMYRRMEEDMDVNCGLVLDGEASVQ
                     EMGARIFQTILDVASGQKTKSELLGYGDEEFVPWQIGAVM"
BASE COUNT         1426 a         3558 c         3584 g         1466 t
ORIGIN      
        1 gcgcgagatc acgcccgacg aggtgctgct gttccgctac tcggcgctca ccttcaacgg
       61 ccaccgcatc cactacgact acccgtacgt caccggcgag gagggctatg agggcctgat
      121 cgtccacggc ccgctcaccg ccaccctgct gctcgacctg gtcgggcgag aagcgccaga
      181 ggcgcgcatc gccggggtgc gcttccgcgc ccaccacccg ctctatgccg ggcgcaagct
      241 caccctcgag gccgcgcccg agtccggcgc catcgcggcc tgggcgctcg attcggccgg
      301 ccgtctcgcg atgcacgcac gcattgagac gaccggacac tgaaaaaagg gggaggctcg
      361 atccatggag tatcgcaggc tcggcaccag cggcgtcacc gtgtcacggc tgtgcctcgg
      421 caccatgatg ttcggcgacc aggcggacga gacggccgcg aagcgcatcg ccgacatggc
      481 gtgggacgcg ggaatcaact tcatcgacac cgccgacgtc tatgccggcg gggcgtcgga
      541 ggaggtcacc ggcaaggtga tccgccgccg ccgcgaccgc tgggtgctgg cgaccaagct
      601 cgggcaagcc gcgcgcgcga acgatgccaa cgccggcggg ctcagccggc gctggatcac
      661 cgctgagctc gaggcgagcc tgaagcggct cggcaccgac catgtcgaca tcctctactt
      721 ccaccgcgag gacctcgcct gcgcgctgga ggagtcgctg cgcgcggtgg gcgatgcgat
      781 ccgcgccggc aaggtgcgct atctcggcct ctccaacttc cgcgcctggc gccacgccga
      841 ggtcgcgcgc ctgtgcgatt cgctcggcat ggaccgaccg gtggcgagcc agccctacta
      901 caacgccttc aaccgcatgc ccgagaccga ggtgctcaag gcctgccagc attacggcat
      961 gggcgtggtg ccctacagcc cgatcgcgcg cggcgtgctc acgggcaagt acccgcccgg
     1021 caaggcgccc gcccgcggca cccgcgccgg gcgtaaggac cggcgcatga tgcagaccga
     1081 gttccgcgag gagtcattgg tgctcgcgca acggatggtg gactatgcga agaggcgcgg
     1141 catgacaccg gtcctcttcg ccgtgcgctg ggtgctcgag aaccccatcg tcaccgcgac
     1201 catcgccggc ccgcgcacgc ccgagcagat gcagggctat ctcggctgct tcgaccacga
     1261 ctggaccgcc gacgacgagg cgttcgtcga ctccctcgtc gcccccggcc acccgtccac
     1321 ccccggctac aacgaccccg gctacgtcat cgagggccga ccggcgcgcg tctgagcggc
     1381 gccggcgcgc ttgaagcgtc ttacgctcag attgattcag ttccccctca cccagctccg
     1441 ggtaaggcgc ggctgacgcc gcaccaaccc tacgcaaccc tctcccccgc gttgcggggg
     1501 agagggaacg gtgaaggggc gatcaataca aattcgaaca tactctatgg ggctgcggcc
     1561 gtcccgcgcg cccgagcttg gcgcgcgcgg cgcgccagca tgttgagggc ctcgaccagc
     1621 gccgagaacg ccatcgccgc gtagacgtag cctttcggca cgtggacgcc gaaaccgtcg
     1681 gcgagcagca gcatgccgat catcatcagg aagccgagcg ccagcatgac cacggtcggg
     1741 ttggcgtgaa taaagtcagc cacgggcgtc gcggcgaaca tcatgaccgc gaccgcgatc
     1801 agcaccgcca ccaccatgat cgggaaatgc gtcgtcatgc cgaccgcggt caggatgctg
     1861 tcgatggaga acacgaggtc gaacaccagg atctgggcga ccgtgacggc gaagcccgcc
     1921 gccgcctgcg ccgcggccga gggctcgacg gtggggtcga cgcggtggtg tatctctatg
     1981 gtcgccttcc acagcaggaa gacgcccccc gccagcataa tcaggtcgcg ccacgagaag
     2041 gcgtgatcga acagcgtcac gatcggctcg gtcagccggg cgatgtaggc gacggacccg
     2101 agcagtgcga gccgcaggat cagggcgaga ccgatcccga ccctacggcc gagcgcgcgc
     2161 tgatgctccg gcaacctgtt ggtcaggatc gagatgaaga tcaggttgtc gatgccgagc
     2221 accacctcca tcgcggtgag cgtggcgagc gccgcccacg cccctgggct ggcggcgaga
     2281 tcaaacaggt actgcatgtc cggaaaaact cccgtcttcg ctgttggcgc gggttacgcg
     2341 gaccggccag tgcgcgaccg actacgagat cgtcaccccg gaagctgcgc agcggctgtc
     2401 cggggtccat ggtccctagc aattggcacg atggatgccg gctctcgctc gcgctcggcc
     2461 ggcatgacgg cgggagcggt gcggcgccat gattcgtgca cgaatcgttg ccgcgggcgg
     2521 tggcctaaag cgcgtcctcc ggcagcgcca tgaccgtctc ggaggcgcac atgatcgggc
     2581 gcgcgagcgc cgcggcgggg gccaggtact gggcggcgaa gtagcgcgcg gtgtccacct
     2641 tggcggtgta gaacgcctcg tcggcggtga agtgctcgag gtgctcggtt gccttggccg
     2701 cggcctcggc catcagccag ccgccggcgg tgaaccccat catgcgcatg tacggcgtcg
     2761 cgcccgccgc cgccgccacc gggtcgtcgc ggaaggtggt ttgcatccac tcggtggtgt
     2821 gcgcgaacgc gtccacggcg ggcgcgagct cggcggcgat cgcgcgcaag ccgtcgtggt
     2881 cggcctgcga gagtcgttcg gcggtcgcgc gcatgtcctc gatcagccgc actaccgcct
     2941 cgccgccgtc ctgttgcagc ttgcgccgca ccaggtcgag cgcctggatg ccgttggtgc
     3001 cctcgtagat catggtgatg cgcgcgtcgc gcaggtgctg ggcggcgccg gtctcctcga
     3061 tatagcccat gccgccgtgc acctggacgc cgagcgaggc gacctcgaag ccctggtcgg
     3121 tgcaccagcc cttggcgacc ggcgtcatca ggtcggcata gccttgcgcg cgcgcgcgcg
     3181 cagcctcgtc aggctcgcgc ttggcgcggt cgagagcggc ggcgaccgag tagcacaaca
     3241 ggcgcatggc ctcggtttgg ctgcgcatgt ccatcagcat gcggcgcacg tcgggatggc
     3301 ggatgatcgc caccggctcg cccgagcgct cgccgatcat ggccccctgg atgcgctggc
     3361 gcgcccagtc gcgcgccgcc tggcaggcgc gctcggcgat ggcgaggccc tggacgccga
     3421 cattgagccg ggcgttgttc atcatcgtga acatgcactg catgccgtgg cgctcgggcc
     3481 cgatcagggt ggcgagcgcg ccctcgtcgt cgccgtagga catgacgcag gtcgggctcg
     3541 catggtggcc catcttgtgc tcgatcgaga ccacgcgcag gtcgttgcgc gcacccagcg
     3601 agccgtcgtc gttgaccagg accttgggca ccaggaacag cgagatgccg cgcgtgccgg
     3661 gcggcgagcc gggcgtgcgc gccagcacca ggtggacgac gttctcggtc atgtcctggt
     3721 cgccgtgggt gatgaagatc ttgctgccgg tgatgcgcca ctcgccgttg gtgcgcaccg
     3781 cgcgggtgcg cagcgcgccc acgtccgagc ccgcattggg ctcggtcagg ttcatggtcc
     3841 ccgaccacgc gccgctcact aatttgggga gatagagtct tttttgctcg tccgagccgt
     3901 ggctctcgat cgcgtccacc gcgccctggg tcagcatcag cccgtcggcg agcgccatgt
     3961 tcgccgactg ccacatctcg ttgagcgcgg tggtgaccgt catgccgagg cccatgccgc
     4021 catgggccgg atcggcgccg gcggcgcccc agccggcctc gacccacctg gcgtacgctt
     4081 cggcgaagcc cgcgggcatg cgcaccacgc cgttgacgaa ctcggcgccc tgcgcatcgc
     4141 ccggctggtt gagcggcgcg agcacgtcgg cggcgaaccg cccggcctcg cccagcacct
     4201 ggtcgaccag atccgccgtg acctggtcga gccccggcag cgccgccagc gcctcgagcc
     4261 cgatcatgtc ggcgagcacg aagcgcatgt cggctaaggg cgcagcgtag cccgtggcca
     4321 tgtctgggtc ctccctgcct gcgcgaaggc aggccgaatc cccgcggcgg tcagaccacc
     4381 ttgccggggt tcatgatgcc ctgggggtcg agcgcggcct tgagcgtgcg catcagcgcg
     4441 acctcgacca ctgagcggta cttcaccagc gccgcgcgct tgagctggcc gatgccgtgc
     4501 tcggcgctga acgagccgcc gagctcggcg accaggtcgt ggatcacctc gttgagctcg
     4561 tcccagcggg cgaggaagtc ctcgcggtcg gcaccgggcg gctggctgac gttgaagtgg
     4621 acgttgccgt cgccgacatg gccgaagggg accggccggc tgcccgggat ggtacgctcg
     4681 gtcagcgcga ccgcgcggcg gatgagctcg ggcaccttgc tcaccggcac ggagatgtcg
     4741 tgcttgatgc tgccgccctc gggcttctgc gcctcgctca catgctcgcg cagcgcccac
     4801 aggttgcgcg cctgcgcctc gctcgcggcg atggtgccgt cgagcaccag gccgtccgcc
     4861 agcgcgcgct cgagcaggcg ctccatcgcc gcctccagcc cggcatccgc cgccggcgag
     4921 ctgagctcgg ccagcacgta ccagtcgtgg cggtcggcga gggggtcctg ggcggcgggc
     4981 atgtgcgcga gcacgaagtc gaggcagatg cgcgccatca gctcgaagcc ggtgaccgaa
     5041 tcgccggtct cctcgcgcgc ttgcgcgagc agctcgatcg cggcgtccgg gtcgcgcacc
     5101 gcgaccatcg cggtcagcac cgagcgcggc ttcggcatca gcttgagcac cgccgcggtg
     5161 atgatgccga gcgtgccctc ggcgcccacg aacaattgct tgaggtcgta gccggtgttg
     5221 tccttgcgca agctcctgag cccgtcccag acgcggccgt ccgccagcac cacctcgagg
     5281 cccagtacct gctcgcgcgc catgccgtag cgcagcacgt tggtgccgcc ggcgttggtc
     5341 gacagattgc cgccgatctc gcagctccct tccgcggcga ggctgagggc gagcatgcgc
     5401 ccggccgcgg cggcggcggc ctgcaggtcg gcgagaatgc agccggcctc ggcgcacatc
     5461 gtgtagttga gccggtcgat gtcgcggatc gcgttcatgc gcgagagatt gacgatgatc
     5521 tcgcggccgt ggccgtgcgg caccgagccg ccgcacatgc tggtgttgcc gccctggggg
     5581 acgacggcga cgccgagatc ggcgcacagg cgcaccacgg ccgccacctc ctcggtgctc
     5641 gccgggcgca ccagcgccgc cgccttgccg cggtacttgc cgcgctggtc gagcaggtgc
     5701 ggcgcgatgt cctcggcgtc ggtgacgagc cccttgtcgc cgacgatcgc gcgcagccgt
     5761 cctgccaggt cgttgaggtc cttgatcgcc agtgcggtca cgaagccatg ccttgtcgca
     5821 cccgatttcg cggatgtagc ccatcgggcc gggcccgaca aggccgcccg ccggcggcta
     5881 gggcgcggcc gtctccgccg cccggcgcag gcggtcgttg atcgccgcgc cgaggcccgt
     5941 ctcggggatc ggcatcaccg cgatcgcggc ggcgccggcc tcgtcgagcg cgtgcagcat
     6001 ggcgaacagg ttggccgccg cctcggtgac gtcgcccgcc gcactcaggt tgagggtggt
     6061 ccgggcgccg ggcggtgcgc ccgggccgaa gacgagcagc gcctcgtccg ggcgcacgct
     6121 ggtggcatcg agccgcaccg gcgtgcgcgg cgcgtagtgg cgcgaaagct ggcccggcgc
     6181 cttcggctgc gcgccctcgg tgccgacggc aacgcgcccg atctcggcct cgatctcggc
     6241 gcgcgtcacc gcgccggggc ggagcagggc ggggggcgcg acggtcaggt cgaggatggt
     6301 ggactcgatg ccgaccgggg tcggcccgcc gtcgaggatc aggtcgaccg catcgcccaa
     6361 cccgtcggcg acgtggccgg cggtggtcgg gcttagttgg cccgagcggt tggcgctcgg
     6421 tgccgcgatc ggccggcccg cggcggcgag caggtcgtgc gcgaccgggt ggccgggaac
     6481 gcgcagcgcc acggtccgga gcccgcacag cgcgagcccc gacagcacgc tgtcgtcgcg
     6541 cttcgccagc accagggtga gcgggccggg ccagaagcgc cgggcaagcg cccgcgcgcg
     6601 cgcatccagc ttcgcgtgtc gcgcggccgc ctcggtgtcg cgaagatgga tgatcagcgg
     6661 gttgaagctg ggccgcgcct tggccgcgaa gatgcgcgcg accgcgtgct cgctggtagc
     6721 gtcggcgccg agcccgtaga cggtctcggt cgggaacgcg accaggccgc cggagcgcag
     6781 gatttcggcg gcgcgcgcga tcgcctgcgc cgaagcgggg acgacccgcg ccgcgctcac
     6841 cggtcgagca agggttcgag ccggtcgagc gcctcgccca gcaacgccgc ggtcttggcg
     6901 tagcagatgc gcaagtaggt ctcgccgccg gcgccgaacg cgatcccggg cgccaggccg
     6961 accttggtct cggccagcac ctgcatggcg aacgccatgc tgtcgtcgat gccgtcgacc
     7021 gcgaagaagc cgtagaacgc cgcctcgggg cgcgacaggc gcacgcggcg catggcgccg
     7081 aggcgctgct ggaccatgtc gcgcgcaatc cggtagcgct cgaccatctc ggcgacaaac
     7141 ggctcgccgt cgcgcaccgc cacgacgccg gcatactggg tgccggtgcc gggcgaggcg
     7201 acgttgtact cgctgagctt ctcgaacgcc tcgccgagcg cgggcggatg ggtgatccac
     7261 cccaatcgcc agccgctcat cgcccacgac ttggagaagc tgttgaccac cagcaccagg
     7321 tcgtcgtcgc tcgcgtggtc gaggaacgac ggcgccaccg ggcggtcgta gacgatgcgc
     7381 gcatagacct cgtcggcgat cagccacagc ccgcggtcgc ggcagaagtc gagcaccgcg
     7441 gcctgggtct cggagtccat catccagccg gtcgggttgt tgggcgagtt gatggtgatc
     7501 gcgcgggtgc gcgcatcgca ggcgtcgaac aggcggtcga gatcgagccg ccagctcccg
     7561 ggttgaaccc gggggtcgcc cgcgatcgtc atcggcacct ggcgcgcctc gccgcccatg
     7621 atctcgatgc agcggaagaa gttgggccac aagggcgtgg caatgacgat gttgtcgccg
     7681 gggtcgacca gcagctcggc gacctgcatc agcgcgttca tgccggcggc ggtcaccgtg
     7741 atccgctcga tgccgatcgc cttgcggtac agccggctgg tgtagtcggc gagcgtctcg
     7801 cgcagctcgg gcacgccgcg attgtggctg tagaaggtgt ggccggcgct gagcgccgcg
     7861 tccgaggcgg cgacgatgaa cgccggggtg gggaggtcgg gctcgccgaa ccacagcggg
     7921 atcaccccgt cctggcccat gccggcgtgg gcgatggcgc gaatcccgga cggctgcagc
     7981 tcggcgacct cgcggcggat cggcagcgcc gcatcgcgcg gcgcgccggc cgcgtcggca
     8041 ctccccttcg ccgcccgcat cagccgcgcg cccctgccgt cgcgctcaca gcctcctcga
     8101 ccgcgtgcac caggtcgcgc ccggcgagcg cgccgtcgcc ggtcatgcgc ccgccggcca
     8161 ccgccttcag cgcctggatg tgcatctcga tcacctcgat ctcgtcctcg cggcccgtgg
     8221 ccgagccaca gaagtcatat agcgagccgg caaggcgcgt gaccagcccg tagccgtaag
     8281 tggtgccgtg ggcgcgcatg gcgcgtgcgc gacgggcgat tgcggcgagt gcggcggcac
     8341 tgccgcgcgc cgcgacgacg ccgagctccg cctcgagcgc ggcgatgtcg gcgaacagcg
     8401 agtcgaggta gcgcgcgccg cgcgcgcgca ccaccgcccg cgcggcctcg accgccgccg
     8461 ggtcgatgcc gccggcacgc acgccgcctt cggtcgcctt ggcgcgcagg tcgggcgtcg
     8521 gcgtatcgct gccggacgtg cgcccgatca tggcgcgccc gcctcgtcgc gccgctcgcc
     8581 gccgcgatac ggcatctcgt ggcggcgccg gtcggggccg aaataggagc gggtgtcgac
     8641 gaactgccgc ggcgcctcga tgatcgcctc cagcaccgcg cacaggctgt ccactgtcac
     8701 cggcttgacc agcacctcgg tcgcgccgcg cgcgcgcacg gcgagcgcgg cctccagatc
     8761 ggtcgcatcg gcgagcacga tcaccggcaa atagcggttg ggcgagcgcg gatggcggcg
     8821 gatccagtcg gtcaaggtcg cccgccggtc aggcgcgacg gcgacgtcgg cgaccaccag
     8881 gtcgatcggc tgggccttgc ggtcggcgag catggtccgt gccacctcgc cgttgggcgc
     8941 ggcgaccgtg cgcgcgaagc cgagcgcggc gaacaggtcg cacagcagcg cggtgatctc
     9001 gggatcgtcg tcgaccacca gcaccgacag ccggtcgagc cgccaatatg cgccggccac
     9061 cgtaccctgc gcgcggccgg cgatcacatc accgcgccga tctgccaggg cacgaactcc
     9121 tcgtcgccgt agcccagaag ctcgctcttg gtcttctgcc cggaggcgac gtcgaggatg
     9181 gtctggaaga tgcgcgcgcc catctcctgg acgctcgcct cgccgtccag caccaggccg
     9241 cagttgacgt ccatgtcctc ttccatgcgc cggtacatgg gcgtgttggt ggcgagcttg
     9301 atcgacggcg tcggcttgca gccgaacgcc gagccgcggc cggtggtgaa gcagaccacg
     9361 ttggcgccgc cggcgaccat gccgttgacc gaggtcacgt cgtagcccgg cgtgttcatg
     9421 aagcagaagc ccttggcggt gaccggctcg gcgtactggt agacgtcgac caggttggtg
     9481 gtgccgccct tggcgaccgc gcccaaggac ttctcgagga tggtggtcag cccgccggcc
     9541 ttgttgccgg gcgaggggtt gttgttcatc tcggcgccgt tcttggcggt gtactcctcc
     9601 caccacagga tatgcttgat cagcttgtcg gcgaccgcct tcgagaccgc gcggcgggtg
     9661 agcaggtgct cggcgccata gatctcgggg gtctcggcga gcaccgcggt gccgccgtgg
     9721 cggaccagca ggtcggccgc ggcgccgaga gcaggattgg cggtgattcc cgaataggcg
     9781 tccgagccgc cgcattcgag cccgaggatc aggtggctcg ccggcaccgt ggtgcggctc
     9841 acccggttgg cctcgggcag catctccttg atccgcgcga cgccctcggc gaccgtcttg
     9901 cgggtgccgc cggtgtcctg gatggtcatg gtcgacagca tcttgccggt ctgccagccg
     9961 tagcgctcga ccatgctcgc gatctggttg acctcgcagc ccaatccgat cgcgagaatc
    10021 ccggcgaagt tggc
//
LOCUS       DASWHU010000015         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2592608, whole genome shotgun sequence.
ACCESSION   DASWHU010000015 DASWHU010000000
VERSION     DASWHU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592608"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1454
                     /locus_tag="VGB88_00565"
     CDS             192..1454
                     /locus_tag="VGB88_00565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9645957.1"
                     /translation="MIGLRVGMPAAAAVLCAVAALAEPLPSAAPEEVGLSGARLARIG
                     EVMGAGIEAGEIPGAVVLVARHGRVAYYEAFGWRDRANGAPMARDAIFRIYSMTKPLI
                     SVGAMMLHEQARLYPSEPLAKYVPAFETMAVGVESFDPVTGRQGFHTEPARRAITIHD
                     LLRHTSGLTYGFFGTSRVKDMIKEAGLDPFTLDMDLAEFSDRLAALPLAYQPGEVWDY
                     SLSADVLGRVVEVASGLPLDQYLRERLFAPLGMVDTDFWVPPAKQDRIAEAQAGDPAD
                     GLIDVTRAPKMFAGGHGLVSTAMDYARFCQMMLARGSLEGTRILGRKTVEYMTSDHLG
                     DISRAGTAYLPGPGYGFGLGFAVRTAAGESPWPGSVGEFFWGGYAGTYFWIDPAEQLV
                     VVYMMQSVAQRQHYRMLLRNLVTQAIVD"
     gene            complement(1560..1766)
                     /locus_tag="VGB88_00570"
     CDS             complement(1560..1766)
                     /locus_tag="VGB88_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645958.1"
                     /translation="MTIQTPLKFAVGVIGLSLLIAAAAPGAPALACAPQIKFDPVTGL
                     ALPDPDLKTALEKINAGLHVADAR"
     gene            complement(1978..2187)
                     /locus_tag="VGB88_00575"
     CDS             complement(1978..2187)
                     /locus_tag="VGB88_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645959.1"
                     /translation="MTATGVARSLFAAAVLSAAAFSLLDAGAARACMPEIVVDPVTGQ
                     AQGQAELEAALAELTRKVELAQAGR"
     gene            2226..>2558
                     /locus_tag="VGB88_00580"
     CDS             2226..>2558
                     /locus_tag="VGB88_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645960.1"
                     /translation="MTAHRPTMRCEPPHTRADAQAGPRRRAPRNRTLDLDAGDAARLR
                     ARLVRAGDVPRLVAAMGAAPLDAVVHGDCREVLPALPHAFADLLILDPPYSSGRRFAG
                     RRFARLSDA"
BASE COUNT          355 a          888 c          953 g          362 t
ORIGIN      
        1 atccggacgg gcgccactac gcggtctcgg gctactggca cgacatcgcg gtcatcgagc
       61 gcggatcgca ccggccggtg cccgggcgcg cgcagcacgc caccctgttc ggccacagcc
      121 acatgaccgc ggcggcggcg aaacgcggct aagctgcacc ggttcgcggg gcgggaaaag
      181 ggaggagaga gatgatcgga cttcgcgtcg gcatgccggc ggcggcggcg gtgctgtgtg
      241 cggtcgccgc gctcgccgag ccgctgccgt cggctgcgcc cgaagaggtc ggcctgtcgg
      301 gcgcgcgcct ggcgcggatc ggcgaggtga tgggcgccgg catcgaggcc ggcgagatcc
      361 cgggcgcggt ggtgctggtc gcgcgccacg ggcgcgtcgc ctactacgag gcgttcggct
      421 ggcgcgaccg cgccaatggt gcgccgatgg cgcgcgatgc gatcttccgc atctactcga
      481 tgaccaagcc gctgatcagc gtcggcgcga tgatgctgca cgagcaggcg cggctgtacc
      541 cgtccgagcc gctggccaag tacgtgcccg cgttcgagac catggcggtc ggggtggaga
      601 gcttcgaccc ggtcaccggc cgccagggct tccataccga gcccgcgcgc cgcgccatca
      661 ccatccacga cctgctccgc catacctcgg gcctgaccta cggcttcttc ggcacctcgc
      721 gggtcaagga catgatcaag gaggcgggcc tcgatccgtt caccctcgac atggacctcg
      781 ccgagttttc cgaccggctg gccgcgctcc cgctcgccta ccagccgggc gaggtgtggg
      841 actacagcct gtcggccgac gtgctgggac gggtggtcga ggtcgcctcg gggctgccgc
      901 tcgaccaata cctgcgcgag cgcctgttcg cgccgctggg catggtcgac accgacttct
      961 gggtgccgcc ggccaagcag gaccgcatcg ccgaggccca ggccggcgac ccggccgacg
     1021 gcctgatcga cgtcacccgc gcgccgaaga tgttcgccgg cggccacggc ctggtgtcga
     1081 cggcgatgga ctatgcccgc ttctgccaga tgatgctggc gcgcggcagc ctggagggga
     1141 cgcgcatcct gggccgcaag acggtcgagt acatgacctc ggaccacctc ggcgatatca
     1201 gccgcgccgg caccgcctat ctgcccggcc ccggctacgg cttcgggctc ggcttcgcgg
     1261 tccgcaccgc ggccggcgag tcgccgtggc cgggctcggt cggcgagttc ttctggggcg
     1321 gctacgccgg cacctatttc tggatcgacc cggcggagca gctggtggtg gtgtacatga
     1381 tgcaaagcgt cgcccagcgc cagcactacc ggatgctgct caggaacctg gtcacccagg
     1441 cgatcgtcga ttgaccgccg cagcgcgcgc aaaaagttcg gccgccgggt ggcggcggaa
     1501 agggagggga gaacggacga tggcagtgtc ggcggagcgg tcgggacggc aggctccgct
     1561 cacctcgcgt cggcgacgtg caggccggcg ttgatcttct ccagcgccgt cttgagatcc
     1621 ggatcgggca gggcgagccc ggtcaccgga tcgaacttga tctggggcgc gcaggcgagg
     1681 gccggggcgc ccggggcggc agcggcgatc agcaacgaca gaccgatgac gccaaccgcg
     1741 aacttcaacg gggtctgaat ggtcatcgct cgggactttc aaagtcttgc tttcggggag
     1801 cgtggtgatc acagcaccaa tcgtgcggca acgatgtgaa ccgcttcaca gggcgagtca
     1861 cgatcccgtg aagcgaccgt tcgccgggca tcgagtctcg ggctcgggcg cggccgccgc
     1921 acgggcggcc gcgagatatc ggggaacgtg gtcgcgatga cgggcggcgc gcggcggtca
     1981 gcggccggcc tgggccagct cgactttgcg agtgagctcg gccagcgccg cctcgagctc
     2041 ggcctggccc tgggcctggc cggtcaccgg gtcgaccacg atctcgggca tgcaggcgcg
     2101 cgccgcgccg gcatcgagca ggctgaaggc ggccgccgac agcacggcgg cggcgaacag
     2161 ggagcgggca actccggtgg cagtcatggt catgctcctc gcctcggggc tcctgaactg
     2221 cggtcatgac ggcacatcgg ccgacgatgc gctgtgaacc gcctcacacg cgggcggacg
     2281 ctcaggcggg gccgcgccgg cgcgcgccgc gcaaccgcac cctcgacctc gatgcgggcg
     2341 atgccgcgcg gctgcgcgcg cgcctggtgc gggcgggcga tgtcccgcga ctggtcgcgg
     2401 ccatgggcgc ggcgccgctc gatgcggtgg tgcacggcga ctgccgcgag gtgctgccgg
     2461 cgctgccgca cgcgttcgcc gacctgttga tcctcgaccc gccctacagc tcgggccgcc
     2521 ggttcgccgg ccggcgcttc gcccgcctgt cggacgcg
//
LOCUS       DASWHU010000016         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2961911, whole genome shotgun sequence.
ACCESSION   DASWHU010000016 DASWHU010000000
VERSION     DASWHU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2961911"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2174)
                     /locus_tag="VGB88_00585"
     CDS             complement(<1..2174)
                     /locus_tag="VGB88_00585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX9645961.1"
                     /translation="MVAYAELQVTTNFSFLRGASHPQELVHAAAALGHAAIAVTDRNS
                     LAGVVRAHAAAKQAGIRLVVGARLEVTDGPSALCLPTNRAAYARLARLLTLGKRRTVK
                     GQCELAWADVEAHAEGQVLVVLPPEAGLPDGAFAEHLAAIARRHPGRSYLAASCRFAG
                     EDAARLAVLDALAAACATPLVATGDVLYHHPGRRRLADVLACIREHCTIDEAGLRLAA
                     NAERYLKPGTEMARLFAHYPNAIARTVEIADACQFSLDELAYDYPDEVVPAGEGAQSW
                     LATLVAEGAAARYPHAVPEKVRAQLDHELKLIGELAYAPYFLTVHDIVRFANSRAILC
                     QGRGSAANSAVCYCLGITAVDPERNDVLFERFVSAARNEPPDIDVDFEHERREEVIQY
                     VYAKYGRDRVGLTATVISYRARSAIREVGKALGLSVDMVGALARGIWGWSREGLDEEQ
                     VRELGLDPGEGRLALALELAGELIGFPRHLSQHVGGFVITRGLLSEMVPIENAAMADR
                     TVIEWDKDDIDALGMLKVDVLGLGMLTCIRKAFALIARHHGRALSLATVPGEDPAVYD
                     MLCRADSIGTFQVESRAQMSMLPRLKPRCFYDLVIEVAIVRPGPIQGDMVHPYLRRRN
                     GEERVDYPSEELERVLGKTLGVPLFQEQAMRIAVVAAGFTPAEADQLRRAMATFRKTG
                     IIHTFRRKLVDGMVARGYEAAFAERCFNQIEGFGEYGFPESHA"
     gene            complement(2451..>3892)
                     /locus_tag="VGB88_00590"
     CDS             complement(2451..>3892)
                     /locus_tag="VGB88_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX9645962.1"
                     /translation="AAVSPAAAGAGLAPGMTLADARALVPGLRVRPAEPLRAARALEA
                     LADWALRFTPWVATSGADGLMLDVSGCAHLFGGEEALLARLVAGVTGQGVAARAALAD
                     TPGAAWACARFGPGLPSPKRSSGFAQAGGVVPAGGSAQALAPLPVAALRLEAATVAAL
                     ERLGLRCIGDLAGKPRAPLAARFGAALVTRLDQAEGRLREPVSPRRPPARHQARRAFA
                     EPVADAPSILAALEAVCAELCASLAHAGLGARRLALTLFRIDGGARTIPVGTSRPVRE
                     ARPLVRLFAEHLDGFDTGAGIEVIALAARTTEALGARQLVSGLEPGIAGGSDDGTALA
                     GLVDRIANRLGPGRIKRLGPRPSHLPDRAVSPLPAVERAPEAGWPARPPRPLRLLSPP
                     LPVEAVAVVPDGPPVLFRWRGRVHRVARASGPERIADEWWHERAPTRDYYRIEDQAGA
                     RFWLYREGLYEDPAAAAPAPRWYLHGLFG"
BASE COUNT          517 a         1417 c         1470 g          488 t
ORIGIN      
        1 gcgtggctct cggggaagcc gtactcgccg aaaccctcga tctggttgaa gcagcgctcg
       61 gcgaaggcgg cctcgtagcc gcgcgcgacc atgccgtcca ccagcttgcg gcggaaggtg
      121 tggatgatgc ctgtcttgcg aaaggttgcc atggcgcggc gcagctggtc ggcctcggcc
      181 ggggtgaagc cggcggcgac cacggcgatg cgcatcgcct gctcctggaa cagcggcacg
      241 ccaagcgtct tgccgagcac gcgctcgagc tcctccgagg gatagtcgac ccgttcctcg
      301 ccgttgcgcc gacgcaggta ggggtggacc atgtcgccct ggatcgggcc cgggcggacg
      361 atcgcgacct cgatcacgag gtcgtagaag catcgcggct tgagcctcgg cagcatcgac
      421 atctgggcgc ggctctcgac ctggaaggtg ccgatcgagt ccgcccggca cagcatgtcg
      481 tagaccgccg ggtcctcccc cggcacggtg gcgagcgaga gggcccggcc gtggtggcgg
      541 gcgatcagcg cgaacgcctt gcggatgcag gtcagcatgc cgaggccgag cacgtcgacc
      601 ttgagcatgc caagggcgtc gatgtcgtcc ttgtcccatt cgatgacggt acggtcggcc
      661 atcgccgcgt tctcgatcgg caccatctcg gacaacagcc cgcgggtgat gacgaagccg
      721 ccgacatgct gcgacaggtg gcgcgggaag ccgatcagct cgccggcgag ctcgagcgcg
      781 agtgcgagcc ggccctcgcc gggatcgagg ccgagctcgc gcacctgctc ctcgtcgagc
      841 ccctcgcggc tccagcccca gatgcccctg gcgagcgcgc cgaccatgtc caccgacagc
      901 cccagtgcct tgccgacctc gcggatcgcg ctcctcgcgc ggtacgagat cacggtcgcg
      961 gtcagcccga cccggtcgcg gccgtacttg gcgtagacgt actggatcac ctcctcgcgg
     1021 cgctcgtgct cgaagtcgac gtcgatgtcg ggcggctcgt tgcgcgccgc cgagacgaag
     1081 cgctcgaaca gcacgtcgtt gcgctcgggg tcgaccgcgg taatcccgag gcagtagcac
     1141 accgccgagt tggccgccga cccccgcccc tggcacagga tggcgcgcga gttggcgaag
     1201 cggacgatgt cgtggacggt gaggaagtag ggcgcgtagg cgagctcgcc gatcagcttg
     1261 agctcgtggt cgagctgggc gcgcaccttc tccggcacgg cgtgcggata gcgcgccgcg
     1321 gcgccctctg cgaccagggt ggcgagccag ctctgcgcgc cctcgcccgc cggcaccacc
     1381 tcgtccgggt agtcgtaggc gagctcgtcg aggctgaact ggcacgcgtc cgcgatctcg
     1441 accgtgcgcg cgatcgcatt tggatagtgt gcgaacaggc gcgccatctc ggtgcccggc
     1501 ttgaggtagc gctcggcgtt ggcggcgagc cgcaagcccg cctcgtcgat ggtgcagtgc
     1561 tcgcggatgc aggcgagcac gtcggcgagc cgcctgcggc ccgggtggtg gtagagcacg
     1621 tcgccggtcg cgaccagcgg cgtagcacac gctgcagcga gcgcgtcgag cacggcgagc
     1681 cgggccgcgt cctcgccggc gaagcggcag ctcgcggcaa gataagagcg tccgggatgg
     1741 cggcgggcga tggcggcgag gtgctcggcg aaggcgccgt cgggcaggcc cgcctccggc
     1801 ggcagcacga ccagcacctg gccctcggca tgagcctcga cgtcggccca ggcgagctcg
     1861 cattggccct tgaccgtccg cctcttgccg agggtgagca ggcgggcgag ccgggcatag
     1921 gccgcgcggt tggtcggcag gcacagcgcg ctcggcccgt cggtcacctc gagccgcgcg
     1981 ccgaccacga gccggatgcc cgcctgcttg gccgcggcgt gcgcgcgcac caccccggcc
     2041 agcgagttgc ggtcggtgac cgcgatcgcg gcatggccga gcgcggcggc agcgtggacc
     2101 agctcctgcg ggtgcgaggc gccgcggagg aagctgaagt tggtcgtcac ctgaagctcg
     2161 gcgtaggcaa ccattgccat ggaatgcttc ttgctggccc tcatgccatt aagcgtatga
     2221 tccgactcag gtcgtcgacg cgacgttgcc gccgatactt tcgtcatgcc ggccttgagc
     2281 cggcatccat cggactgtgc cggcggcacg atggaccccg gattcgccct gcgggcgctc
     2341 cggggtgacg ggaggagaag ccgcggctgc ccagtcagcc ggcgcgcgta cggttcggct
     2401 catcggctcg ctggccatga cgttctcccg atctcgctcg cccttgtccc ttacccgaac
     2461 aggccgtgca ggtaccagcg cggcgcgggg gcggcggcgg cggggtcctc gtacaggccc
     2521 tcgcggtaga gccagaagcg ggcaccggcc tggtcctcga tgcggtagta gtcgcgggtc
     2581 ggcgcccgct cgtgccacca ctcgtcggcg atgcgctcgg gccccgaggc gcgcgccacc
     2641 cggtgcaccc ggccgcgcca gcggaacagc accggcggcc cgtcggggac caccgccacc
     2701 gcctccaccg gcagcggcgg gctgagcagg cgcagcggcc tgggcggccg ggcgggccag
     2761 cccgcttccg gcgcgcgctc caccgccggc agggggctga ccgcgcggtc gggcaggtgg
     2821 ctcggccgcg gccccagccg cttgatgcgc cccggcccga gccggttggc gatgcggtcg
     2881 accaggccgg cgagcgcggt gccgtcgtcg ctgccgccgg cgatccccgg ctctaacccg
     2941 gagacgagct ggcgcgcgcc cagggcctcg gtggtgcgcg cggcgagcgc gatcacctcg
     3001 atcccggcgc cggtatcgaa gccgtcgaga tgctcggcga acaggcgcac cagcggccgc
     3061 gcctcgcgca ccggccggct ggtgccgacc gggatggtgc gcgcgccgcc gtcgatccgg
     3121 aacagggtca gcgcgagccg gcgcgcgccg agcccggcat gggcgaggct tgcgcacagc
     3181 tcggcgcaga ccgcctcgag cgcggcgagg atcgagggcg catccgcgac cggctcggcg
     3241 aaggcgcggc gggcctggtg gcgcgccggc gggcgcctgg gcgagaccgg ctcgcgcagc
     3301 cgcccctcgg cctggtcgag gcgggtgacg agggcggcgc cgaagcgcgc ggcgagcggc
     3361 gcccgcggct tgccggcgag atcgccgatg cagcgcaggc caaggcgctc gagcgcggcg
     3421 acggtggcgg cctccagcct cagcgccgcg accggcagcg gcgccagcgc ctgcgccgag
     3481 ccccccgccg gcaccacgcc gcctgcctgc gcgaagccgg agcttcgctt cggcgaaggc
     3541 aggccggggc cgaagcgcgc gcacgcccac gccgcgccgg gggtgtcggc gagcgcggcg
     3601 cgcgccgcca ccccctggcc cgtcactccc gcgacgaggc gggcgagcag cgcctcctcg
     3661 ccaccgaaca ggtgcgcgca gcctgagacg tcgagcatca gcccgtctgc gccgctggtc
     3721 gccacccatg gagtgaagcg cagcgcccag tcggccagcg cctcgagcgc gcgcgccgcg
     3781 cgcagcggct cggccgggcg cacgcggagg cccgggacca gcgcgcgcgc gtccgccaag
     3841 gtcatgccgg gggcgagccc ggcgcccgcc gccgccggcg agaccgcggc ca
//
LOCUS       DASWHU010000017         8021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2962364, whole genome shotgun sequence.
ACCESSION   DASWHU010000017 DASWHU010000000
VERSION     DASWHU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962364"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGB88_00595"
     CDS             complement(<1..485)
                     /locus_tag="VGB88_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9645963.1"
                     /translation="MAVNRQITLAARPEGYPKPSDFKLVEGPVPEPKDGQMLVRALWL
                     SLDPYQRGRMSAARSYAASIEIGAPIIGGIVGRVETSHNPRFAAGDIVEGWLGWQDYA
                     LSDGHDVRKVDPQLAPIQTALGVLGMPGMTAYFGLLEIGRPRPGDTVVVSAASGAVGS
                     LV"
     gene            complement(538..831)
                     /locus_tag="VGB88_00600"
     CDS             complement(538..831)
                     /locus_tag="VGB88_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645964.1"
                     /translation="MRALAALVAAALAAPAAAEEAGQAPLEPMLERAARIVITECRPD
                     DATLVYDASKDHVVAGNVLAQRFDARCPDSDQTITIICFSPMVGRHTPECMRR"
     gene            complement(828..1835)
                     /gene="thiS"
                     /locus_tag="VGB88_00605"
     CDS             complement(828..1835)
                     /gene="thiS"
                     /locus_tag="VGB88_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX9645965.1"
                     /translation="MHITVNGERRDIPAPITVARLIDQLGLDARKIAVERNRELVRRS
                     AFAETPLGDGDALEIVHFIGGGAGALAELAPTAAADDDGWTVAGRRLTSRLIVGTGKY
                     QSLEQTAAAIEASGAEMVTVAVRRVNVTDPKAPMLMDHVDPRKYVYLPNTAGCFTAED
                     ALRTLRLAREAGGWTLVKLEVLGDQKTLYPNMIETLSAAETLIGDGFEVMVYCNDDPI
                     MAKRLEEIGCSAIMPLAAPIGSGLGIQNPVHIRIIVENASVPVIVDAGVGTASDAAEA
                     MELGCDAVLMNTAIAGARDPILMARAMRHAVQAGRLAYRAGRMAKKLYADPSSPLAGL
                     I"
     gene            2064..2507
                     /gene="aroQ"
                     /locus_tag="VGB88_00610"
     CDS             2064..2507
                     /gene="aroQ"
                     /locus_tag="VGB88_00610"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX9645966.1"
                     /translation="MARTVLVLNGPNLNMLGTRQPDIYGAETLADVEALCHQAARALD
                     MTVSFRQTNLEGELVTWIQEARGDFDGLLINAGGYTHTSIAIMDALLASEVPAVEVHM
                     SNIHAREPFRRRSYVALAARGSICGFGADGYRLGLQALHGMLAEA"
     gene            2510..2968
                     /locus_tag="VGB88_00615"
     CDS             2510..2968
                     /locus_tag="VGB88_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9645967.1"
                     /translation="MADKLTVDDDLIRRLARLLEETGLSEIEIGEGQQHVRVARADGG
                     GALGAVPLPADTVAALPEDSQPAATEVDASHPGAVVSPMVGTLYLAPEPGAPPFAALG
                     STIREGDTMFIIEAMKTMNPVRAPRDGKVTRVLVSNETPVEYGEVLALVE"
     gene            2991..4346
                     /gene="accC"
                     /locus_tag="VGB88_00620"
     CDS             2991..4346
                     /gene="accC"
                     /locus_tag="VGB88_00620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9645968.1"
                     /translation="MFEKVLIANRGEIAMRIHRACHEMGIRTVAVHSTADAEAMHVRL
                     ADESVCIGPPPARASYLNVPSIISACQVTGAEAIHPGYGFLSENARFAEIVAEHGFTF
                     IGPEPAHLRLMGDKVAARRAMRELGIPLVPGTEEAVRDAKSARAFAERIGYPVLIKAA
                     CGGGGRGMKVARDGRELEAMLDLAAREAFEAFGDGTVFLEKYLTGPRHIEIQVLADQH
                     GHVMHFGERDCSLQRRYQKLVEEAPSPALDPATRADIGGRAAEAVRKLGYHGLGTLEF
                     LFEDGAFYFIEMNTRLQVEHPVTEMITGIDLVREQIRVAAGVPLGFSQGDIRFSGHAI
                     ECRITAEDPVTFMPRPGRVTDYHPPGGLGVRVDSALYAGYTVPPHYDSMVAKLIVHGS
                     NRTECLMRLRRSLEEFVIDGIPTTLPLHRRLVEAPDFADGLYDIGWLERFVAQPAEAA
                     S"
     gene            4372..5070
                     /gene="aat"
                     /locus_tag="VGB88_00625"
     CDS             4372..5070
                     /gene="aat"
                     /locus_tag="VGB88_00625"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX9645969.1"
                     /translation="MRPLQITADTLLRAYQWGLFPMGENRDDPTIYWVDPEQRGVLPL
                     DRFHLPRSLRRSVRRKSFALRVDSAFDEVIRACAQPYPGRYTTWINTTIERLYLDLFE
                     MGYAHSVECWRDGALAGGLYGVAIGGAFFGESMFSRESDASKVALAHLVVRLRIGGYD
                     LLDTQFVTEHLARFGAVEVPRARYLRMLARALKIKGDFYRLDSPKSSDSSGASGAASS
                     ADWTALALSPPAED"
     gene            complement(4965..5498)
                     /locus_tag="VGB88_00630"
     CDS             complement(4965..5498)
                     /locus_tag="VGB88_00630"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX9645970.1"
                     /translation="MAALLAAAAPACAQPAGRDATFLFGDDWLKSEMAKLEALDKVTG
                     RVSEIEVGVDRPARFGTLDIVVRACHRRPPELPPDSAAFLQIAERRYPGDPPETLFSG
                     WMFASSPGLSALEHPVYDVIVLDCLRAPEGEEGTATAADAAQSSAGGDSASAVQSADD
                     AAPEAPEESLDFGESSR"
     gene            complement(5536..5802)
                     /locus_tag="VGB88_00635"
     CDS             complement(5536..5802)
                     /locus_tag="VGB88_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645971.1"
                     /translation="MSEKSVEVGRKPGQGAGAPADLLPTWLAPDGAPIACVEKIKMLN
                     DNILEIRQLCRDALEDAVLMGCDETQIRKVLLRLVRSLEEPFRD"
     gene            complement(5822..6268)
                     /gene="mlaD"
                     /locus_tag="VGB88_00640"
     CDS             complement(5822..6268)
                     /gene="mlaD"
                     /locus_tag="VGB88_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX9645972.1"
                     /translation="MRRNIVETLIGAVVLVVAIGFFTYAYSTADVGAVAGYPVLAKFT
                     SVGSLAPGADVRISGIKVGSVVRQRLDPETYMAEVTMSLRRDIRLSADTTAAVGMEGL
                     LGGAYVTLEPGGEEEMIAPGGEILFTQAAPDIIALVTQLVFSQKEK"
     gene            complement(6332..7141)
                     /locus_tag="VGB88_00645"
     CDS             complement(6332..7141)
                     /locus_tag="VGB88_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9645973.1"
                     /translation="MTVWAILFAPFVLILLIAVTHVYFGLHVLARGIIFVDLALAQIA
                     AVGASLAFLLGYDGHGAWAQAAALAAALLASLAFAQLRRVPDTVTREVVIGCAYVVAV
                     ALSIMILSRSVTGMEELKTLLNGNILWVGWRDIALLAAVYAAVGAIHVVWRHRFLELS
                     RAGERAGGRALAWETLFFTSFAVVITLAVMVAGVLVVFALLIIPAFSATLLARGFARR
                     LAAGWALAALGCGVGLALAYVADLPVGAAVVSVLGLVPVTAAVLSRRSGPA"
     gene            complement(7143..>8021)
                     /locus_tag="VGB88_00650"
     CDS             complement(7143..>8021)
                     /locus_tag="VGB88_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX9645974.1"
                     /translation="GLLAVGRPADAALSVLTTTTDLAAIAQAVGGELVDAESLTPGTR
                     DPHFAEARPSMIRKIYGADLLLVVGAQLEIGWLPAALQAGRNARLTPGNPGYLDLSAT
                     VRLLDVRTGEISRAMGDVHAAGNPHYWLDPRNGRPVARAIAARLVELDPDNAGAYQQR
                     LGAFEQQLDQGLGRWRAAMAPFAGRKLLSYHRSMIYLADAFEFELVGEIEPLPGISPT
                     ASHLADLVNGVRADNVAGILIEPFYDPKPAEFVGRQTGAPVIVMPQSVGALPGIATYF
                     DLFDAIVAAFARAGTT"
BASE COUNT         1190 a         2877 c         2816 g         1138 t
ORIGIN      
        1 accagcgagc cgaccgcgcc tgaggccgcg gacaccacca cggtgtcgcc gggccgcggt
       61 cggccgatct ccaagagccc gaaataggcg gtcatgcccg gcatgccgag caccccgagc
      121 gcggtctgga tcggcgccag ctgcggatcg accttgcgca cgtcgtggcc gtccgacagc
      181 gcgtagtcct gccagccgag ccagccctcg acgatgtcgc cggccgcgaa ccgcgggtta
      241 tggctcgtct ccacccggcc gacgatgccg ccgatgatcg gcgcgccgat ctcgatgctc
      301 gccgcgtagg acctggcggc gctcatccgc ccgcgctggt aggggtcgag cgacagccac
      361 aaggcgcgga ccagcatctg cccgtccttg ggctccggca ccgggccttc gaccagcttg
      421 aagtcgctcg gcttcgggta gccctcgggg cgcgccgcga gcgtgatttg cctgttgacc
      481 gccatccctc atgccctccc cggcgcgatc aggctgagct tgcgcccgcc cgcgatatta
      541 gcggcgcata cactccggcg tgtgccggcc gaccatcggc gagaagcaga tgatggtgat
      601 ggtctggtcg ctgtccggac agcgtgcgtc gaagcgttgc gcgagcacgt tgccggccac
      661 cacgtggtcc ttgctcgcat cgtagaccag ggtggcgtcg tcggggcggc actcggtgat
      721 cacgatccgg gcggcgcgct ccagcatcgg ctcgagcggc gcctggcccg cctcctctgc
      781 ggcggccggc gcggcgagcg cggccgcgac cagcgccgcc agcgccctca tatcaggccg
      841 gcgagcggcg aggacgggtc cgcgtagagc ttcttcgcca tccgcccggc gcggtaggcg
      901 agccggccgg cctggaccgc gtggcgcatg gcgcgggcca tcaggatcgg gtcgcgggcg
      961 ccggcgattg cggtgttcat cagcaccgcg tcgcagccga gctccatcgc ctcggccgcg
     1021 tccgaggcgg tgccgacgcc ggcgtcgacg atcaccggca ccgaggcgtt ctcgacgatg
     1081 atccggatgt ggaccgggtt ctggatgccg agccccgagc cgatcggcgc cgccagcggc
     1141 atgatcgcag agcagccgat ctcctccagc cgcttggcca tgatcgggtc gtcgttgcag
     1201 tagaccatca cctcgaagcc gtcgccgatc agcgtctcgg cggcgctcag ggtctcgatc
     1261 atgttgggat agagcgtctt ctggtcgccc agaacctcga gcttgaccag ggtccagccg
     1321 cccgcctcgc gcgccagccg caaggtgcgc agcgcgtcct cggcggtgaa gcagccggcg
     1381 gtgttcggca gatagacgta tttgcgcggg tccacatggt ccatcagcat cggcgccttg
     1441 ggatcggtga cgttgacccg gcgcacggcg acggtgacca tctcggcgcc cgaggcctcg
     1501 atcgcggccg cggtctgctc gaggctctgg tacttgccgg tgccgacgat caggcgcgag
     1561 gtaagcctgc gcccggcgac cgtccagccg tcgtcgtccg ccgccgcggt cggcgcaagc
     1621 tccgccagcg cgcccgcgcc gccgccgatg aagtggacga tctccagcgc atcgccgtcg
     1681 ccgagcgggg tctcggcgaa ggccgagcgg cgcaccagct cgcggttgcg ctcgaccgcg
     1741 atcttgcgcg catccagccc gagctggtcg atcaggcggg cgaccgtgat cggcgctggg
     1801 atgtcgcggc gttcgccgtt gacggtgatg tgcataccgt gcctctcgcg ggacgtggct
     1861 gcccggctct tgcccagagc agcttttgct cagagtatgg gagccgggcg cgggcgattc
     1921 aaccgagccg cccgccgccc gtgcggcgcg cgttgcgcag ccggcgccgc gccatgctat
     1981 aaggcgggcg gggcgatggt cgcccgagcg gcgccgacgc gcgccgccgg gcggtgttgc
     2041 cgcgatcgat cctggccctg gcaatggcgc gcaccgtcct ggttctcaac ggccccaacc
     2101 tcaacatgct gggcacccgc cagccggata tctacggcgc cgagaccttg gccgacgtcg
     2161 aggcgctgtg ccaccaggcc gcgcgtgcgc tcgacatgac cgtgtccttc cgccagacca
     2221 acctcgaggg cgagctggtg acctggatcc aagaggcgcg cggcgatttc gacggcctgc
     2281 tgatcaatgc cggcggctac acccacacct cgatcgcgat catggacgcg ctgctcgcca
     2341 gcgaggtgcc ggcggtggag gtccacatgt cgaacatcca cgcgcgcgag ccgtttcgcc
     2401 gccggtccta tgtcgcgctc gccgcgcgcg gctcgatctg cggcttcggc gccgacggct
     2461 accggctggg cctgcaggca ctccacggca tgctggcgga ggcctgatga tggccgacaa
     2521 gctgaccgtc gacgatgacc tgattcgccg cctcgcccgc ctgctcgagg agacggggct
     2581 gagcgagatc gagatcggcg agggccaaca gcacgtgcgg gtggcgcgcg ccgacggcgg
     2641 cggcgcgctg ggcgcagtgc cgctgccggc cgataccgtc gccgcgctcc ccgaggattc
     2701 gcagccggcc gcgacggagg tcgacgccag ccatccgggc gcggtggtct cgcccatggt
     2761 cggcaccctc tacctcgcgc ccgagccggg cgcgccgccg ttcgccgccc tcggcagcac
     2821 catccgggaa ggcgacacga tgttcattat agaggcgatg aagaccatga atcccgtgcg
     2881 cgcgccgcgc gacggcaagg tcacccgcgt gctggtgagc aacgagacgc cggtcgagta
     2941 cggcgaggtc ctcgcgctcg tcgagtagca cgcgagccgc ccccccgccc atgttcgaaa
     3001 aggtgctgat cgccaatcgc ggtgagatcg ccatgcgcat ccatcgcgcc tgccacgaga
     3061 tgggcatccg cacggtcgcc gtgcattcca ctgccgacgc cgaggcgatg cacgtgcgcc
     3121 tcgccgacga atcggtgtgc atcggcccgc cgccggcgcg cgccagctac ctcaacgtgc
     3181 cctcgatcat ctccgcctgc caggtgaccg gggccgaggc gatccatccg ggctacggct
     3241 tcctgtcgga gaacgcgcgc ttcgccgaga tcgtcgccga gcacggtttc accttcatcg
     3301 ggccggagcc cgcgcatctg cgcctgatgg gcgacaaggt cgcggcgcgg cgggcgatgc
     3361 gcgagctcgg catcccgctg gtgccgggca ccgaggaggc ggtccgcgac gccaagtcgg
     3421 cgcgcgcctt cgccgagcgg atcggctatc cggtgctgat caaggcggcg tgcggcggcg
     3481 gcgggcgcgg catgaaggtg gcgcgcgacg ggcgcgagct cgaggcgatg ctcgacctcg
     3541 ccgcgcgcga ggcgttcgag gcgttcggcg acggcaccgt gttcctcgag aagtacctca
     3601 ccggcccgcg ccacatcgag atccaggtgc tcgccgacca gcacggccac gtcatgcact
     3661 tcggcgagcg cgactgctcg ctgcagcgcc gctaccagaa gctggtcgag gaggcgccct
     3721 cgcccgcgct cgatccggcg acccgcgcgg acatcggcgg ccgcgccgcc gaagcggtgc
     3781 ggaagctcgg ctatcacggc ctcggcacgc tcgagttcct gttcgaggac ggcgcgttct
     3841 atttcattga gatgaacacc cgcctgcagg tcgagcaccc ggtcaccgag atgatcaccg
     3901 gcatcgacct ggtgcgcgag cagatccggg tcgcggccgg ggtgccgctc ggcttctcgc
     3961 agggcgacat ccgcttcagc ggccatgcca tcgagtgccg gatcaccgcc gaggacccgg
     4021 tcacgttcat gccccgcccg gggcgggtga ccgactacca tccgccgggc gggctcggcg
     4081 tgcgcgtcga ttcggcgctt tacgccggct acacggtgcc gccccattac gacagcatgg
     4141 tcgccaagct gatcgtgcac gggtcgaacc gcaccgagtg cctgatgcgc ctgcgccgca
     4201 gcctggagga gttcgtcatc gacggcatcc cgaccacgct gccgctgcac cgccggctgg
     4261 tcgaggcgcc ggacttcgcc gacggcctgt acgacatcgg ctggctcgag cggttcgtcg
     4321 cccagccggc cgaggccgcg tcctagggga gttgcccgcg tcatcgaccc gttgcgcccg
     4381 cttcagatca ccgccgacac cctgttgcgc gcctaccaat gggggctgtt cccgatgggc
     4441 gagaaccggg acgatccgac catctactgg gtcgacccgg agcagcgcgg ggtgctaccg
     4501 ctggaccgct tccacctgcc gcgcagcttg cgccgcagcg tgcggcggaa gtcgttcgcg
     4561 ctgcgcgtgg actcggcgtt cgacgaggtc atccgcgcct gtgcgcagcc ctatccgggc
     4621 cgctacacga cctggatcaa caccaccatc gagcggctct atctcgacct gttcgagatg
     4681 ggctatgcgc actcggtcga gtgctggcgc gacggcgcgc tcgccggcgg cctgtacggg
     4741 gtggcgatcg gcggcgcgtt cttcggcgag agcatgttca gccgcgagag cgacgccagc
     4801 aaggtcgcgc tcgcccacct ggtggtgcgc ttgcgcatcg gcggctacga cctgctcgac
     4861 acccagttcg tgaccgagca cctggcccgc ttcggggcgg tcgaggtgcc gcgggcgcgc
     4921 tatctgcgca tgctcgcccg cgcgctcaag atcaagggcg acttctaccg gctggactcg
     4981 ccgaaatcga gcgactcctc gggcgcctcc ggcgcggcgt cgtccgcgga ctggacggcg
     5041 ctggcgctgt cgccgccggc cgaggactga gccgcgtccg ccgccgtcgc cgtgccctcc
     5101 tcgccctccg gcgcgcgcag gcagtcgagc acgatcacgt catagaccgg gtgctcgagc
     5161 gccgacaggc ccgggctcga ggcgaacatc cagccgctga acagggtctc gggcggatcg
     5221 cccggatagc ggcgctcggc aatctgcagg aacgcggcgc tgtccggcgg cagctcgggc
     5281 gggcgccggt ggcaggcgcg caccacgatg tcgagcgtgc cgaaccgtgc cggccggtcg
     5341 acgccgacct cgatctccga cacccggccg gtgaccttgt cgagcgcctc gagcttcgcc
     5401 atctcggact tgagccagtc gtcgccgaac agaaaggtcg cgtcccgccc ggcgggctgg
     5461 gcgcaggccg gcgccgcggc ggcgagcagc gcggccacgc aggccgcaag cgcgggccgg
     5521 tggcgacgcg ggcggtcagt ccctgaacgg ttcctcaagc gagcgcacca ggcgcagcag
     5581 caccttgcgg atctgcgtct cgtcgcagcc catcagcacc gcgtcctcga gcgcgtcccg
     5641 gcacagctgg cgaatctcga ggatgttgtc gttgagcatc ttgatcttct cgacgcaggc
     5701 gatcggcgcg ccgtcgggcg cgagccaggt cggcagcagg tcggcgggcg cgccggcgcc
     5761 ttgccccggt ttcctgccaa cttcaactga tttttcagac attgtagata agtctctgat
     5821 tttatttttc tttctggctg aacaccagct gcgtcaccaa ggcgatgatg tcaggtgcgg
     5881 cctgggtgaa caggatctcg ccgccgggcg cgatcatctc ctcctcgccg cccggctcaa
     5941 gcgtaacata ggcaccgccg agcagccctt ccatgccgac ggccgcggtg gtgtcggcgg
     6001 acaggcggat gtcgcgccgg aggctcatgg tgacctcggc catgtaggtc tcggggtcga
     6061 ggcgctggcg gaccaccgag ccgaccttga tgccgctgat gcgcacgtcc gcgcccggcg
     6121 ccaacgagcc gacgctggtg aacttggcca acaccggata gcccgcgacg gcgccgacgt
     6181 cggcggtcga gtaggcatag gtgaagaagc cgatggcgac caccagcacc accgcgccga
     6241 tcagagtctc gacgatgttg cgtctcatgg cgcccccatc gcgcggcggt tgatgccgcg
     6301 cgcgccaata tcggccaagg ccggacccaa ttcaagccgg cccggaccgg cgggacaaga
     6361 ccgcggcagt caccggcaca aggccgagca ccgacacgac cgcggcgccg accggcaggt
     6421 ccgcgacata ggcgagggcg agcccgacgc cgcagccgag cgccgccagc gcccaccccg
     6481 ccgccagccg ccgcgcgaac ccgcgcgcca gcagcgtcgc gctgaacgcg ggaatgatga
     6541 gcagcgcgaa caccaccagc accccggcga ccatcaccgc gagcgtgatc accaccgcga
     6601 acgaggtgaa gaacagcgtc tcccacgcga gcgcccgtcc gcccgcgcgc tccccggcgc
     6661 ggctgagctc gaggaaccgg tgccgccaga cgacatgaat cgcgccgacc gccgcataga
     6721 ccgcggcgag aagcgcgatg tcgcgccagc cgacccacag gatattgccg ttgagcaggg
     6781 tcttgagctc ctccatgccg gtgacggacc ggctgaggat catgatcgac agcgcgaccg
     6841 cgaccacgta ggcgcagccg atcacgacct cgcgcgtgac cgtgtccggg actcggcgca
     6901 gctgcgcgaa ggcgagcgag gcgagcagcg ccgcggccag cgccgctgcc tgcgcccacg
     6961 cgccgtggcc gtcgtagccg agcaggaaag cgaggctggc ccctaccgcc gcgatctggg
     7021 ccagcgccag atcgacgaag atgatgccgc gggcgagcac gtgcagcccg aaatagacgt
     7081 gcgtcacggc gatcaggagg atcagcacga acggcgcgaa caggatcgcc cagaccgtca
     7141 tgtcacgtgg tcccggcacg cgcgaacgcg gcgacgatgg cgtcgaacag gtcgaaatag
     7201 gtcgcgatgc cgggcagcgc gcccaccgac tgcggcatca cgatcaccgg cgcgccggtc
     7261 tggcggccga cgaactcggc cggcttgggg tcgtagaacg gctcgatcag gattccggcg
     7321 acgttatccg cgcggacccc gttgaccagg tccgcgaggt gcgacgccgt cggcgagatg
     7381 ccgggcaagg gctcgatctc gccgaccagc tcgaactcga acgcgtcggc gagatagatc
     7441 atcgaccggt ggtaggatag caacttccgg ccggcgaacg gcgccatggc cgcccgccag
     7501 cggccgagcc cctggtcaag ctgctgctcg aacgcgccga gccgctgctg atatgcgccg
     7561 gcgttgtccg ggtcgagctc gaccagccgg gcggcgatgg cccgcgcgac cggccggccg
     7621 ttgcgcgggt cgagccagta gtgcgggttg ccggcggcat gcacgtcgcc catggcgcgg
     7681 gagatctcgc cggtccgcac gtcgagcagc ctcaccgtcg ctgacaggtc gagatagccc
     7741 gggttgcccg gcgtgagccg cgcattgcgc ccagcctgga gcgccgccgg cagccatccg
     7801 atctcaagct gcgcgccgac cacaagcagc agatcggcgc cgtagatctt acggatcatg
     7861 ctcggcctgg cctcggcgaa atgcgggtcg cgggtgcccg gggtgaggct ttccgcatcg
     7921 accagctcgc cgccgaccgc ctgggcgatg gcggcgaggt cggtggtggt ggtgagcacg
     7981 gagagcgcgg catcggccgg ccggccaacc gccagcaggc c
//
LOCUS       DASWHU010000018         7546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2962375, whole genome shotgun sequence.
ACCESSION   DASWHU010000018 DASWHU010000000
VERSION     DASWHU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962375"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2369)
                     /gene="mutS"
                     /locus_tag="VGB88_00655"
     CDS             complement(<1..2369)
                     /gene="mutS"
                     /locus_tag="VGB88_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9645975.1"
                     /translation="MLAQYLAIKRAHGDALLFYRMGDFYELFFDDAVKAAEALDITLT
                     RRGRLDGEDVPMCGVPVHSHEVYLERLIEKGFKVAICEQTEDPAEARKRGPKALVARA
                     VARIVTPGTLTEDGLLDARAHNHLAALARASGGLGLAWLDISTGDVQVASPEPGTLAS
                     LLAQVSPGELLVAEPLLADPQLSQALADWRAVLAPEPQARFDSAAGERRLCRAFAVAT
                     LDAFGAFARAEVAACGALLDYVELTQQGRLPRLSPPRRQEPGAVMAIDAATRRNLELT
                     QAAQGGREGSLLAVIDRTRTGAGARLLAQRLAAPLTDPAAIGRRLDAVARFADDDGLR
                     RAVREALHRAPDIARALARLSLGRGGPRDLAAVRDGAARARAVRALLERAAGTGLAPL
                     PDELAGCAAALGEHGVLIDRLERALAQDLPPGARDGGVIARGYAPALDEQRSLRDESR
                     RLVAALQLRYREETGIASLKVRHNNVLGYFVEVTASHGDKLLGRAGFIHRQTLASAVR
                     FATEELARLEDQIARAADRALALELELFDDLVGEVRARERELAPAARALAELDVAAAL
                     AELARVRSYVRPHVDQSLDFRIAKGRHAVVEAALGERAFVANDCELSGAGRLWLLTGP
                     NMAGKSTFLRQNALIAVLAQAGSFVPAEACEIGVVDRLFSRVGAADDLARGRSTFMVE
                     MVETAAILNQAGPRALVILDEIGRGTATYDGLSIAWATIEHLHDQNRCRGLFATHYHE
                     LTGLSGRLDALACHTMRVREWQGAAVFLYEVAPGAADRSYGIHVAKLAGL"
     gene            2559..4886
                     /locus_tag="VGB88_00660"
     CDS             2559..4886
                     /locus_tag="VGB88_00660"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9645976.1"
                     /translation="MRTGGRAMAQASSSYHTTDAEALAFHAGGKPGKLEIRATKPLTT
                     SRELSLAYSPGVAVPCLEIKRDPSLSFDYTSRGNLVAVISNGTAVLGLGNLGALASKP
                     VMEGKAVLFKRFADIDGIDLEVATADVDEFVNCVRFLGNSWGGINLEDIKAPECFIIE
                     QRLRECMDIPVFHDDQHGTAIIVAAGLVNALHLTGRRLEDARLVVNGAGSAGIACLEL
                     LKRMGLPHDHGLLCDTKGVIYVGREAGMNQWKSAHAVDTELRTLAEAVAGADVFFGLS
                     AKGAMTKDMVRAMADAPIIFALANPDPEITPEEVAEVRGDAIVATGRSDYPNQVNNVL
                     GFPYIFRGALDVRATTINDEMKVAAVHALASLAREDVPDEVAAAYSSQHLQYGPSYIL
                     PTPFDPRLIVALPVAVARAAMGSGVAQKPIADFEEYAHQLEARLDPTATHMHMVQARL
                     QGRACRMVFAEGEEEKIIRAAVQWQGNGFGTPVLIGREDRILETMKRIGLRQPEGLEM
                     HNARLSKDNTTYTEFLYARLQRKGYLYRDCQRLVNQDRNVFGACMVAHGHADAMITGL
                     TRSYYVALEDICRVIDPNPGEITFGLSIMISRERTVFIADSTVNELPTPEQLADIACQ
                     SATKAREMGHEPRVALLSFSNFGNPPLEAARRVREAVDVLDSRRVGFEYDGELAADVA
                     LNPALRALYPFCRLSGPANVLIMPALHSANIATKLLQELGGGTLVGPLLMGLEKPAQI
                     VPMGATVSDVVHMATFAAHEAIRNQAADPVRAAAS"
     gene            4923..6326
                     /locus_tag="VGB88_00665"
     CDS             4923..6326
                     /locus_tag="VGB88_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9645977.1"
                     /translation="MRGVRARLLGAAQAALAVSIPYGIVVLVLAAFGRLPLDWGLLAW
                     ALGAAGAGAVLWPLVQGLARVVLYLEAVVEGGAAGAPSFAWLGLGRALSAAMRHLDAG
                     WRSQSRALADSLAARRTLLDALPDPLLLLSRERRVVFANAAARAMIDADPQGRDLAEV
                     LRVPGVLAAAERVWGGEAASEVSFQTGPEPRRHYIGRLQALRAPPPQDSAVALVLHDV
                     TALERGQRQRADFVANASHEIRTPLAAIVGAIETVQGPARDDAEARARFLAMIAEHAE
                     RITRLVDDLLSLSRIEMAEHEPPAGAVALAPVLDQARTALDWRARAREVAIVLDLSPG
                     LPEVQGDADELRQLFQNLIDNAIKYGNPNAEVTVSARPVAEGTRVDSWRAAPGAVAVT
                     VADRGPGIAPEHLPRLTERFYRVDRGRSRQMGGTGLGLAIVKHIVNRHRGALQVTSEL
                     GVGSRFTVCLNGAPEGE"
     gene            6476..6679
                     /locus_tag="VGB88_00670"
     CDS             6476..6679
                     /locus_tag="VGB88_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645978.1"
                     /translation="MVATFMTGHFELFGLDQVIRHFRGREPAVPRSATPGFQRYVRFP
                     IQAGFIVAFWASPMKTTAGECQC"
     gene            complement(6751..7122)
                     /locus_tag="VGB88_00675"
     CDS             complement(6751..7122)
                     /locus_tag="VGB88_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010319432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9645979.1"
                     /translation="MDVTPIKSKRDYRRALKEIEGLMDAKRGTPEGDRLDVLVTLVEA
                     WEAKHYPIDLPDPIEAIKYHMEQNALKPRDLIPFIGSRNRVYEVLNRRRPLTLQMVWR
                     LHAGLGIPAESLIKPADGQAA"
BASE COUNT         1057 a         2672 c         2770 g         1047 t
ORIGIN      
        1 aggccggcga gcttcgccac gtggatgccg taggagcggt cggcggcgcc gggggcgacc
       61 tcgtagagga acaccgccgc gccttgccac tcgcggaccc gcatggtgtg gcaggcgagc
      121 gcatcgagcc ggccgctgag cccggtgagc tcgtggtagt gggtcgcgaa caggccgcgg
      181 cagcggttct gatcgtgcag gtgctcgatc gtcgcccagg cgatcgagag gccgtcatag
      241 gtcgccgtgc cgcgcccgat ctcgtccagg atcaccagcg cgcgcgggcc ggcctggttg
      301 aggatcgccg cggtctcgac catctcgacc atgaaggtcg agcgcccgcg ggcgaggtcg
      361 tcggcggcgc cgacccggct gaacaggcgg tcgacgacgc cgatctcgca cgcctctgcg
      421 ggcacgaacg agccggcctg cgcgagcacc gcgatcagcg cgttctggcg gaggaaggtg
      481 ctcttgcccg ccatattggg gccggtcagc agccacagcc gccccgcccc cgacagctcg
      541 cagtcgttgg cgacgaacgc gcgctcgccg agcgccgcct cgaccaccgc gtgccggccc
      601 ttggcgatgc ggaagtcgag gctctgatcc acatgcgggc gcacgtagct gcggacgcgg
      661 gcgagctcgg cgagggcggc ggcgacgtcg agctcggcga gcgcgcgcgc ggcgggcgcg
      721 agctcacgct cgcgggcgcg cacctcgccc accaggtcgt cgaacagctc gagctcgagc
      781 gcgagcgcgc ggtcggcggc gcgcgcgatc tggtcctcga gccgggcgag ctcctcggtc
      841 gcaaaccgca ccgcgctcgc gagcgtttgg cggtggatga agcccgcgcg cccgagcagc
      901 ttgtcgccgt ggctcgcggt cacctcgacg aagtagccga gcacgttgtt gtggcgcacc
      961 ttgagcgagg cgatgccggt ctcctcgcga tagcggagct ggagcgcggc aaccagccgg
     1021 cggctctcgt cgcgcaagct ccgctgctcg tcgagcgccg gcgcatagcc gcgggcgatg
     1081 acgccgccgt cgcgcgctcc gggcggcagg tcctgggcga gcgcgcgctc gagccggtcg
     1141 atgagcaccc cgtgctcgcc gagcgcggcg gcgcatcccg caagctcgtc gggcaacggc
     1201 gcgaggccgg tgccggcggc gcgctccagc agcgcccgga cggcgcgcgc gcgcgccgcc
     1261 ccgtcgcgca ccgcggcgag gtcgcgcggg ccgccgcgcc cgaggctcaa gcgggcgagg
     1321 gcgcgggcga tgtcgggcgc gcggtggagc gcctcgcgca ccgcccggcg caggccgtcg
     1381 tcgtccgcga agcgcgccac cgcatcgagc cggcgcccga tcgcggccgg gtcggtgagc
     1441 ggcgcggcga gacgctgggc gagcagccgc gcgccggcgc cggtgcgggt gcggtcgatg
     1501 accgcaagca aggagccctc gcgcccgccc tgggcggcct gggtgagctc gaggttgcgc
     1561 cgggtcgccg cgtcgatcgc catcaccgcc cccggctcct ggcggcgggg cgggctcagg
     1621 cgcggcagcc ggccctgctg ggtgagctcg acatagtcga gcagcgcgcc gcacgccgcg
     1681 acctcggcgc gggcgaaggc gccgaaggca tcgagggtgg cgacggcgaa ggcgcggcac
     1741 aggcggcgct cgccggcggc cgaatcgaag cgcgcctggg gctccggcgc cagcaccgcg
     1801 cgccagtcgg cgagcgcctg ggagagctgc gggtccgcca gcagcggctc ggcgaccagc
     1861 agctcacccg gcgacacctg ggcgagcagg ctggcgagcg tgccgggctc ggggctcgcg
     1921 acctggacgt cgccggtcga gatgtcgagc caggcgaggc cgaggccgcc ggacgcgcgc
     1981 gcgagcgcgg ccaggtggtt gtgggcgcgg gcgtccagca ggccgtcctc ggtcaaggtg
     2041 ccgggggtga cgatgcgcgc caccgcgcgc gcgaccagcg ccttgggccc gcgcttgcgc
     2101 gcctccgccg gatcctcggt ctgctcacag atcgcgacct tgaagccctt ctcgatcaaa
     2161 cgttcgagat agacctcatg gctgtgcacc ggcaccccgc acatcggcac gtcctcgccg
     2221 tcgagccggc cgcgccgggt gagcgtgatg tcgagcgcct cggccgcctt gaccgcgtcg
     2281 tcgaaaaaca gctcgtaaaa gtcgcccatg cggtagaaca gcagcgcatc gccatgcgcc
     2341 cgcttgatcg ccagatactg cgccaacatc ggggtcgggg cagcgcgaag ggcgccgtcg
     2401 gggcggtcgg gggcggggcg ggaactcggg tcggcggtca tgggcgctgg caggatgtgg
     2461 ccgggaaagc gcgccggcgc gccggcaccg gccgcgagcc gcgctttacg ccgccgcgac
     2521 gggactctag agtggatcgc ctcacaagaa cagggccgat gagaaccggc ggccgcgcca
     2581 tggcgcaagc gagttccagc taccacacca ccgacgccga ggcgttggcg ttccacgccg
     2641 gcggcaagcc cggcaagctc gagatccggg cgaccaagcc gctgaccacc tcgcgcgagc
     2701 tgtcgctcgc ctactcgccg ggggtggcgg tgccgtgcct cgagatcaag cgcgacccgt
     2761 cgctgtcctt cgactacacc tcgcgcggca acctggtggc ggtgatctcg aacggcaccg
     2821 cggtgctcgg cctcggcaat ctcggcgcgc tcgcctcgaa gccggtgatg gaaggcaagg
     2881 cggtgctgtt caagcgcttc gccgacatcg acggcatcga cctcgaggtc gccaccgcgg
     2941 acgtcgacga gttcgtcaac tgcgtccgct tcctcggcaa cagctggggc ggcatcaacc
     3001 tcgaggacat caaggcgccc gagtgcttca tcatcgagca gcgcctgcgc gagtgcatgg
     3061 acatcccggt gttccacgac gaccagcacg gcaccgcgat catcgtcgcg gccgggctgg
     3121 tcaacgcgct gcacctgacc gggcgcaggc tcgaggacgc gcgcctggtg gtcaacggcg
     3181 cgggctccgc cggaatcgcc tgcctggagc tgttgaagcg catgggcctg ccccacgacc
     3241 acggcctgct gtgcgacacc aagggcgtga tctatgtcgg gcgcgaggcg ggcatgaacc
     3301 agtggaagtc ggcgcacgcg gtcgataccg agctgcgcac gctggccgag gcggtcgccg
     3361 gcgccgacgt cttcttcggc ctgtcggcga aaggcgcgat gaccaaggac atggtccgcg
     3421 cgatggctga tgcgccgatc atcttcgcct tggccaaccc cgaccccgag atcacgcccg
     3481 aggaggtggc cgaggtgcgc ggtgacgcga tcgtggccac cggccggtcg gactatccga
     3541 accaggtcaa caacgtgctc ggcttcccct acatcttccg cggcgcgctc gacgtgcgcg
     3601 cgaccaccat caacgacgag atgaaggtcg ccgcggtgca cgcgctcgcc agcctcgcgc
     3661 gcgaggacgt gcccgacgag gtggcggcgg cctattcctc gcagcacctg caatacggcc
     3721 cgtcctacat cctgcccacg ccgttcgacc cgcgcctgat cgtggcgctg ccggtggcgg
     3781 tggcgcgcgc ggcgatgggc tcgggcgtcg cccagaagcc gatcgccgac ttcgaggagt
     3841 atgcgcacca gctcgaggcg cggctcgacc cgaccgcgac ccacatgcac atggtccagg
     3901 cgcgcctcca ggggcgcgcg tgccggatgg tgttcgccga gggcgaggag gagaagatca
     3961 tccgcgccgc cgtccagtgg cagggcaacg gcttcggcac gccggtgctg atcgggcgcg
     4021 aggaccgcat cctggagacc atgaagcgca tcgggctgcg ccagcccgag gggctggaga
     4081 tgcacaacgc gcgcctcagc aaggacaaca cgacctatac cgagttcctg tacgcgcgct
     4141 tgcagcgcaa gggctatctg taccgcgact gccagcggct ggtgaaccag gaccgcaacg
     4201 tgttcggcgc gtgcatggtc gcccacggcc acgccgacgc catgatcacc gggctgacgc
     4261 gcagctacta cgtcgcgctc gaggacatct gccgggtgat cgaccccaac ccgggcgaga
     4321 tcaccttcgg gctgtcgatc atgatcagcc gcgagcgcac cgtgttcatc gccgattcga
     4381 cggtcaacga gctgccgacc ccggaacagc tcgccgacat cgcctgccag agcgcgacca
     4441 aggcgcgcga gatgggccac gagccgcggg tggcgctgct gtcgttctcg aacttcggca
     4501 acccgccgct ggaggccgcg cgccgggtgc gcgaggcggt cgacgtgctg gactcgcgcc
     4561 gggtcggctt cgagtatgac ggcgagctcg ccgccgacgt ggctctcaac ccggcgctgc
     4621 gcgcgctgta tccgttctgc cgcctgtcgg gcccggccaa cgtgctgatc atgcccgcgc
     4681 tgcacagcgc caacatcgcg accaagctgt tgcaggagct cggcggcggc accctggtcg
     4741 gcccgctgtt gatggggctc gagaagccgg cgcagatcgt gccgatgggg gcgacggtgt
     4801 ccgacgtggt ccacatggcc acgttcgccg cccatgaagc gattcggaac caggcggcgg
     4861 acccggtccg ggccgcggcg agctgagacg cgcgcgccgc gcacgcatcg gcctgagccg
     4921 gcgtgagggg cgtgcgcgcg cgcctgctcg gggccgccca ggcggcgctg gcggtgtcca
     4981 ttccgtacgg catcgtcgtg ctcgtcctgg ccgccttcgg ccggctgccg ctcgactggg
     5041 ggctgctcgc ctgggcgctc ggcgccgccg gcgcgggcgc ggtgctgtgg ccgctggtgc
     5101 agggtctggc gcgcgtcgtc ctgtacctgg aggccgtggt cgagggcggc gccgcgggcg
     5161 cgccgagctt cgcctggctc gggctcgggc gggcgctgtc ggcggcgatg cgccatctcg
     5221 atgcgggctg gcgcagccag agccgcgcgc tggctgactc gcttgccgcc cggcgcacct
     5281 tgctcgacgc gctgcccgac ccgttgctgc tgctgtcgag ggagcgcagg gtggtgttcg
     5341 ccaacgccgc cgcgcgggcg atgatcgacg ccgacccgca ggggcgcgac ctcgccgagg
     5401 tgctgcgcgt gccgggggtg ctcgccgccg ccgagcgggt atggggcggc gaggcggcga
     5461 gcgaggtgtc gttccagacc gggcccgagc cccggcgcca ctacatcggc cgcctgcagg
     5521 cgttgcgcgc gccgccgccc caggacagcg cggtagcgct ggtgctccac gacgtcaccg
     5581 cgctcgagcg cggccagcgc cagcgggccg acttcgtcgc caatgccagc catgagatcc
     5641 gcaccccgct cgccgccatc gtcggcgcga tcgagaccgt ccagggaccg gcccgcgacg
     5701 atgcggaggc gcgcgcgcgc ttcctcgcca tgatcgccga gcacgccgag cgcatcacgc
     5761 gcctggtcga cgacctgctg agcctgtcgc ggatcgagat ggccgagcac gagccgccgg
     5821 cgggcgcggt cgcgctcgcc ccggtgctcg accaggcgcg caccgcgctc gactggcggg
     5881 cgcgcgcgcg cgaggtcgcc atcgtcctcg acctcagccc agggctgccc gaggtccagg
     5941 gcgatgccga cgagctgcgc cagctgttcc agaacctgat cgacaacgcg atcaagtacg
     6001 gcaaccccaa cgccgaggtg acggtgagcg cgcgtcccgt ggccgagggc acccgggtcg
     6061 acagctggcg ggcggcgccc ggcgcggtcg cggtcacggt cgccgaccgc ggcccgggca
     6121 tcgcgcccga gcacctgccc aggctcaccg agcgcttcta ccgggtcgac cgcggccggt
     6181 cgcgccagat gggcggcacc gggctcggcc tcgccatcgt caagcacatc gtcaaccgcc
     6241 accgcggcgc gctccaggtc acgagcgagc tcggggtcgg cagccgcttc accgtctgcc
     6301 tcaacggcgc gccagagggg gagtagccgc cgccgcaaac ctatcgacgc gcgctcgaat
     6361 cgcccccgcg gcggtttcct ggcagcgttc ccggcgtggc ggaaacgccg ccggggaagc
     6421 ctctgtcgac gggggaaagc ttccgaaacg gttcgagttc ggttggggca ttgtggtggt
     6481 cgcgaccttc atgaccggcc acttcgagct gttcgggctc gatcaggtga ttcgtcattt
     6541 ccggggccgc gaacccgccg tgccacgatc ggcgacgccg ggtttccaga ggtacgtccg
     6601 tttcccgatt caggcaggct tcatcgtggc gttctgggcg agccctatga agacgaccgc
     6661 cggcgagtgc caatgctgag tcccggcctc aagcgcaaca gggcagcccc gtcgtccgcg
     6721 gaggattgac cgcggcggcg atccccgcgc tcaggccgcc tggccgtcgg cgggcttgat
     6781 cagcgattcg gccgggatgc cgagcccggc gtggaggcgc cagaccatct gaagagtcag
     6841 cggacggcgg cggttgagca cctcgtacac ccggttgcgg ctgccgataa aggggatcag
     6901 gtcgcgcggc ttcaacgcgt tctgctccat gtggtacttg atcgcctcga tcgggtcggg
     6961 caggtcgatc gggtaatgct tggcttccca cgcctcgacc aaggtgacca gcacgtcaag
     7021 ccggtcgcct tccggagtac cgcgcttcgc gtccatgagc ccctcgatct ccttgagcgc
     7081 ccgacggtaa tctcttttgc tcttgatcgg cgtcacgtcc atgggctggc tcctcaggtg
     7141 ctgtgaagaa accgctgagc cctcccttgt tcgtcattcc cgcgaaagcg gtaatccaga
     7201 cttttcaacg agttactgga ctcccgcttc cgcgggagtg acgacatggg gtcaaggccg
     7261 gggattcttt cacgagcttt catgttgcgg tggccaatat ttgggtccgc tgtgtttgtc
     7321 acgaaaccgt catcgaagct tcacaggatg gtggcggggg ctccttaagc acggaggttg
     7381 gcggcgccgc ccggccgagg cgcgcctgcc gtccccgtcc atcggcccca agttgcgagg
     7441 agaggcaatg gtgaagtcca gaactctggc gcttgctgcg ctcgccgcga tcgcggccgc
     7501 gggcgcggcc gaggcccgcg atcagattcg agtggtcggg tcgtcg
//
LOCUS       DASWHU010000019         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2962630, whole genome shotgun sequence.
ACCESSION   DASWHU010000019 DASWHU010000000
VERSION     DASWHU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962630"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VGB88_00680"
     CDS             <1..1068
                     /locus_tag="VGB88_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645980.1"
                     /translation="ALAILAGIERDVAAVEARGERQEIATALAGARARAGDIDGALEA
                     ATATPVLEARAASLAYVAAARAARGDAAGAQATAGEAYRTYLQRPSAGAWFADATEGM
                     APGAGAVVDGVDGEGAFAAAGIGVGEVVVQFDGIVVGSADQLERLVGSRPAGTPIEVA
                     VASDAGTRLARLVLGRADSAEQPTLSVLAAARAASGDVAGAVETADRIADSGDRIATH
                     SMAAAMRASAGDGPGATVAADAACANAERLDATDSGSWSTAAARCAEARMAAGDGGGA
                     IAQARRMTARYDAYETAWIENIALLLSGRARLAFLRLSGDPLASFEVEAPPAYLEAAL
                     LAESGRIEESLEAADRLLTNR"
     gene            complement(1098..>4311)
                     /locus_tag="VGB88_00685"
     CDS             complement(1098..>4311)
                     /locus_tag="VGB88_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645981.1"
                     /translation="GGRQAGEELPVVGVRVGEHEAFGRVVFDWSREVGYRVERDGDTA
                     VLRFELPARLDLADARSNLPPEIVSIEEIVGGPPAVAIGLAAGAHLRDYRVGLKVVVD
                     VFPAIPPETAEVEPEEAPDRSDTAVVVVEPVEPEPPAPAAAEAAAPPADAPAPEPETS
                     AAVAAEEPPSAPLPLVARAPRHDTSPWQALRSLDKPDAPAAAQAAPEAVAEPAAEPAP
                     ESVAEPAVAEPEPVLPADPEAPGLAAAMRAEAPAPGAGDVTLLVGFDVLPDGLDLVFD
                     WPADVRAAVFARAGHLWVVFDAPAEFNFRLLREDLPAIAKAEQVPLVGAAAARFVLSG
                     RFRAQVMRDGDRWRVRLGPKGGAAAALGVTVEGDSEVGARLIVAEPAPGNAFVLTDPE
                     VGDQLTVVPVAGAGRGITPPRHLVDLELLETVQGVVVRPISDTVAVRSLRDVIEITDL
                     AGLELASLAEDVEAGEERGPLTPPPVIEPPPLARPPSPDLDPTAMMLAADDQPVFDFA
                     AWRNVSGAGFLAAKHRLNDRLAALDAEGRGAGRLALARFHLADAQAAEALGWLALALA
                     EDPRLEQDRNVRALRGAANLLQGRLTEARSDLFDPAFDLSREMRLWRAAWLAEQGDMA
                     SANQAFLAGGEVPAGYPRRVASRFRLLAAEAAARAGDVDRAHLMLDALQRDPRERAAV
                     DRISYVRGLAYANAGDTERALDMLARAGSGSDREVRARAERDRVELMLAKGLVTREQA
                     VEALDGLRFAWRGDALELDLLQRIAELQIGVGNYAAGLATYRQAIAVFPDSPDVRMIA
                     SVMNDTFKRLFLGGLGDELSPLEALAIYYDFRELTPVGAVGDQMIYRLADRLIEVDLL
                     EQAATVLQHQVDHRLRGPDLARVGARLASIYLLDKKPALAVRALKASEATAMADELAR
                     QRRELLVRALTGTGDYDRALDLLYGDPGFEADRMRAEIYWRKQDWLSTATVTARMLAR
                     RPPEDTLTASESDDLMRMAVALVLTNNRGALIELKDRYGEALAHSGRAEAFKAIASYA
                     DAAPVSPRAISAAVAEIDSYEAFLASLRKEVSENELAPAE"
BASE COUNT          579 a         1615 c         1593 g          524 t
ORIGIN      
        1 gcactggcga tccttgcagg catcgaacgg gatgtggccg cggtcgaggc gcggggcgag
       61 cgccaggaga tcgcgacggc gctcgccggg gcgcgcgcgc gcgcgggcga catcgacggc
      121 gctctcgagg ccgcgacggc gacgcctgtg ctcgaggcgc gcgccgcgag cctcgcctac
      181 gtcgccgcgg cacgggcggc acgcggggac gccgcaggcg cgcaagccac ggcgggcgag
      241 gcgtatcgga cctatctcca gcgcccgtcg gcgggggcat ggttcgccga cgcgaccgaa
      301 ggcatggcgc cgggcgcggg cgcggtggtc gatggcgtcg atggcgaagg cgcgttcgcc
      361 gcggccggca tcggcgtcgg cgaggtcgtc gtccagttcg acggcatcgt ggtcggctcg
      421 gccgatcagc tcgagcgact ggtcgggtca aggccggccg gaaccccgat cgaggtcgcg
      481 gtcgcgagcg atgcggggac gcgcctcgcg cgcctggtcc tcggtcgggc ggactcggcc
      541 gagcagccga cattgtccgt gctcgcggcc gcgcgcgcgg cgagcggaga cgtggccgga
      601 gcggtcgaga ccgccgaccg gatcgccgat tcgggtgacc ggatcgcgac ccattccatg
      661 gccgcggcca tgcgggcttc agccggcgac gggcccggcg cgacagtcgc ggccgatgcc
      721 gcctgcgcca atgccgagcg cctcgatgca accgacagcg ggagctggag taccgcggcc
      781 gcgcgctgcg ccgaggcccg gatggcggcg ggcgacggcg gcggcgcaat cgcgcaggca
      841 cgccggatga ccgcgcggta cgacgcctat gagactgcgt ggatcgagaa catcgccctg
      901 ttgctgtccg gccgggccag gctcgccttc ctgcgcctga gcggcgatcc gttggcaagt
      961 ttcgaggtcg aggcgccccc cgcctatctc gaagcggccc tgctcgccga aagcgggcgg
     1021 atcgaggaat cgctcgaggc ggccgacagg ctgctcacaa accgttagcg acgcaaggat
     1081 cgatgcgccg ccgcggctca ctccgccggt gcgagctcgt tctcggacac ctccttgcgc
     1141 aacgaggcaa ggaaggcctc gtaagaatct atctcggcga ccgcggccga gatcgcgcgc
     1201 gggctcacgg gcgcggcatc ggcatagctc gcgatcgcct tgaacgcctc ggcccggccc
     1261 gagtgcgcca gcgcctcgcc gtagcggtcc ttgagctcga tcagcgcgcc gcggttgttg
     1321 gtcagcacca gcgccaccgc catgcgcatc aggtcgtcgc tctcggacgc ggtaagcgtg
     1381 tcctccggcg gccggcgcgc gagcatgcgc gcggtcacgg tcgcggtcga cagccagtcc
     1441 tgcttgcgcc agtagatctc ggcgcgcatg cggtcggcct cgaagcctgg gtcgccatac
     1501 aagagatcga gcgcgcggtc gtagtcgccg gtcccggtca gcgcgcgcac caacagctcc
     1561 cggcgctggc gggcaagctc gtccgccatg gccgtcgcct cgctcgcctt cagcgcgcgc
     1621 accgcaagcg ccggcttctt gtcgagcaga tagatcgagg cgagccgggc gccgacccgc
     1681 gcaaggtcag gcccgcgcag gcgatggtcg acctgatgct ggagcaccgt cgccgcctgc
     1741 tcgagcaggt cgacctcgat cagccggtcg gcgaggcgat agatcatctg gtcgccgacc
     1801 gcgccgaccg gggtgagctc gcgaaagtcg taatagatcg ccagcgcctc gagcgggctc
     1861 agctcgtcgc cgaggccgcc caggaacagc ctcttgaagg tgtcgttcat gacgctggcg
     1921 atcatgcgca cgtcgggcga gtcggggaac accgcgatcg cctggcgata ggtggcgagc
     1981 ccggcggcgt aattgccgac gccgatctgg agctcggcga tgcgctggag caggtcgagc
     2041 tcgagcgcgt ccccgcgcca ggcgaagcga agcccgtcga gtgcctcgac cgcctgctcg
     2101 cgcgtcacca gacccttggc cagcatcagc tcgacccggt cgcgctcggc gcgcgcgcgc
     2161 acctcgcggt cgctgccgct gccggcgcgg gcgagcatgt cgagagcgcg ctcggtatcg
     2221 ccggcattgg cgtaggcgag gccgcggaca tagctgatgc ggtcgacggc ggcgcgctcg
     2281 cgcgggtcgc gctgcagcgc gtcgagcatg aggtgcgcgc ggtccacgtc gcccgcccgc
     2341 gccgcggcct cggcggcgag caggcggaac ctcgacgcga cccggcgcgg atagcccgcc
     2401 ggcacctcgc ccccggcaag gaacgcctgg ttggccgagg ccatgtcgcc ctgctcggcc
     2461 agccacgccg cgcgccacag ccgcatctcc cggctgagat cgaaggcggg gtcgaacagg
     2521 tccgagcgcg cctcggtgag ccggccttga agcaggttcg ccgcgccccg caacgcgcgc
     2581 acgttgcggt cctgctcgag ccgcggatcc tcggcgagcg cgagcgcgag ccagcccagc
     2641 gcctcggccg cctgcgcgtc ggcgagatgg aaccgcgcca gcgccagccg gccggcgccg
     2701 cgcccctcgg cgtcgagcgc ggcgagccga tcgttgagcc ggtgcttggc ggcgaggaat
     2761 ccggcgcccg agacgttccg ccaggcggcg aagtcgaaca ccggctgatc gtcggcggcc
     2821 agcatcatcg ccgtcgggtc gagatccggc gacggcggcc gggcgagagg cggcggctcg
     2881 atcaccggcg gcggggtcag cggcccgcgt tcttcgccgg cctcgacatc ctcggcgagc
     2941 gaggcgagct cgagcccggc aaggtcggtg atctcgatca cgtcgcgcaa gctgcgcacc
     3001 gccacggtat ccgagatcgg gcgcacgacg acgccctgga cggtctcgag caactcgagg
     3061 tcgaccaggt gccgcggcgg cgtgatgccg cgcccggcgc cggccaccgg caccacggtc
     3121 agctggtcgc cgacctcggg atcggtgagc acgaacgcgt tgcccggcgc cggctcggcg
     3181 acgatcaggc gcgcaccgac ctcgctgtcg ccctcgaccg tgacccccag cgccgccgcc
     3241 gcgccgcctt tcgggccgag ccgcacccgc cagcgatcgc cgtcgcgcat gacctgcgcc
     3301 cggaagcgtc cgctgagcac gaaccgcgcc gccgcggcgc cgacgagcgg cacctgctcg
     3361 gccttggcga tcgccggcag gtcctcgcgc agcaggcgaa aattgaactc ggccggcgca
     3421 tcgaacacca cccacagatg gccggcgcgg gcgaacaccg cagcccgcac gtcggccggc
     3481 cagtcgaaca ccaggtcgag gccgtcgggc aggacatcga acccgaccag caaggtgacg
     3541 tcgcccgcgc cgggcgccgg tgcctcggcg cgcatggctg cggccaggcc cggcgcctcc
     3601 ggatcggccg gcagcaccgg ctccggctcc gccacagccg gctcggccac cgattcgggc
     3661 gcgggctccg ccgccggctc cgcgactgcc tctggcgcgg cctgtgccgc cgccggcgcg
     3721 tcgggcttgt ccagggaccg aagcgcctgc cacgggctgg tatcgtggcg cggcgcgcgc
     3781 gcgaccaggg gcagcggcgc cgacggcggc tcctcggccg cgaccgccgc gctcgtctcc
     3841 ggctcgggcg cgggcgcatc cgccggcggg gccgcggcct ccgccgccgc gggcgcgggc
     3901 ggttccggct cgaccggctc gaccaccacc actgcggtgt cgctccggtc cggtgcctcc
     3961 tcgggttcga cctcggcggt ctcgggcggg atcgccggga acacgtcgac caccaccttc
     4021 aggccgaccc ggtagtcgcg cagatgcgcc cccgctgcca gaccgatggc gacagcgggc
     4081 ggcccgccca cgatctcctc gatcgagacg atctccggcg gcaggttgga ccgtgcgtcg
     4141 gcgaggtcga gccgcgccgg aagctcgaag cgcagcaccg cggtgtcgcc gtcgcgctcg
     4201 acccgataac cgacctcgcg cgaccagtcg aacaccaccc ggccgaacgc ctcgtgctcg
     4261 ccgacccgca cgcccaccac cggcagctcc tcgcccgctt ggcggccgcc t
//
LOCUS       DASWHU010000020         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_6438, whole genome shotgun sequence.
ACCESSION   DASWHU010000020 DASWHU010000000
VERSION     DASWHU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6438"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VGB88_00690"
     CDS             <1..734
                     /locus_tag="VGB88_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX9645982.1"
                     /translation="AGGRRDLVVVCDHASNRVPAELAGLGLDAALLERHIGWDIGAGA
                     LARRLADLLDAPAVLAAFSRLVIDPNRALDHPQSIPPESDGVPVPGNQGLDAAARAHR
                     VACYFDAYHGAIEARISAALEVGVPALLSIHSFTPVMNGSARPWHAAVLHDDELRLAA
                     PVLEAFRRYPDLIVGDNVPYTGTSELTYTVPHHASRRGLPNVVIEVRQDLIDTPQGVE
                     RWAYLLGDVLAGPLADDRHRRIAAS"
     gene            811..1098
                     /locus_tag="VGB88_00695"
     CDS             811..1098
                     /locus_tag="VGB88_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX9645983.1"
                     /translation="MDDAKRTEIEAAAFRRLVQHLRERTEVQNLDLMELAGFCRNCLS
                     KWYRAEAETRGEDISYEDAREIVYGMPYESWKAKYQAEQPKARLDNLAHDH"
     gene            complement(1129..2022)
                     /gene="folD"
                     /locus_tag="VGB88_00700"
     CDS             complement(1129..2022)
                     /gene="folD"
                     /locus_tag="VGB88_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX9645984.1"
                     /translation="MTEARIIDGKALAARLRAHTARQVAALAAAGGPTPGLAVVLVGE
                     DAASEVYVRNKGRAAEAAGMASVSRRLHASASTGDVLAEVAALNADRAVHGILVQLPL
                     PPQVDAGAVTEAVDPAKDVDGFHPLNAGRLLAGRPTLVPCTPRGCMALLDEALGELAG
                     AEALVIGRSTIVGKPMALLLLAANCTVTLAHSRTRDLPAVCRRADIVVAAVGRPEMVR
                     GDWIKPGAAVIDVGVNRVAAADGSARLVGDVAFAEALKVAGAITPVPGGVGPMTIAML
                     LSNTLQAACAQAGVAVPAPAD"
     gene            2141..2214
                     /locus_tag="VGB88_00705"
     tRNA            2141..2214
                     /locus_tag="VGB88_00705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2173..2175,aa:Gln,seq:ttg)
     gene            complement(2335..2526)
                     /locus_tag="VGB88_00710"
     CDS             complement(2335..2526)
                     /locus_tag="VGB88_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645985.1"
                     /translation="MMATIGSTGRTSMPRKPNYRFERMERERVKAAKKAARAKVKAEK
                     AEKKKEGQAEQVTTTESEA"
     gene            2726..3988
                     /locus_tag="VGB88_00715"
     CDS             2726..3988
                     /locus_tag="VGB88_00715"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9645986.1"
                     /translation="MGELFDVARRRLDEAAQLIDIDPDVLEKLRYPKETLAASLAVRM
                     DDGARKSFKAWRCRYDDTRGPTKGGIRYHPAVNIDEVMALAFWMTFKCAVVDLPFGGA
                     KGGVAVDPKRLSRAELERLSRAFAHAFANFIGPERDIPAPDVYTNAMVMGWIADEYSS
                     IVGRPSPAVVTGKPIVLGGSRGRDSATGRGGYVTLRHLEHEFGFRPETTRVAIQGFGN
                     AASHCARLLHGDGYKVVGVSDSRSALYDPDGLDPAAVLDHKRRTGAVAGAPGRSRPRE
                     LSNQELLEAECDVLIPAALENQITAANAAAVQARVVLEIANGPTTPDADAELARRGVA
                     LVPDILANAGGVTVSYFEWVQNRSGLYWSEDEVARRLGEFMLPNVDAVWGIAREREVS
                     LRTAAYVHALGRIAEAVAAHGTKAYFMS"
     gene            4201..>4317
                     /locus_tag="VGB88_00720"
     CDS             4201..>4317
                     /locus_tag="VGB88_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9645987.1"
                     /translation="MHAITIGLDIAKSVFQVHGEDAQGRIVLQKRLRRSQVVP"
BASE COUNT          617 a         1542 c         1548 g          610 t
ORIGIN      
        1 atgcgggcgg ccggcgcgac ctggtggtgg tgtgcgatca tgccagcaac cgcgtgcccg
       61 cggagctcgc cggcctcggc ctggatgcgg cgctgctcga gcgccatatc ggctgggaca
      121 tcggcgccgg cgcgctcgcc cgcaggctcg ccgacctgct cgacgcgccg gcggtgctgg
      181 ccgcgttctc gcgcctggtg atcgatccca accgcgcgct cgaccacccg cagtcgatcc
      241 cgccggagag cgacggggtg cccgttcccg gcaaccaggg cctcgatgcc gcggcgcgcg
      301 cgcaccgcgt cgcctgctac ttcgacgcct atcacggcgc catcgaggcg cgcatctccg
      361 ccgcgctcga ggtcggcgtg ccggcgctcc tctccatcca cagcttcacg ccggtcatga
      421 acggcagcgc gcggccgtgg cacgcggcgg tgctgcacga cgacgagctc aggctggcgg
      481 cgccggtgct ggaggcgttc cgccgctacc ccgacctgat cgtcggcgac aacgtgccct
      541 ataccggcac cagcgagctc acctacaccg tcccccacca cgccagccgg cgcggactgc
      601 ccaacgtggt gatcgaggtc cgccaggacc tgatcgacac gccgcagggt gtcgagcggt
      661 gggcgtacct gctcggcgac gtgctcgccg ggccgctcgc cgacgaccgc caccgccgga
      721 tcgcggcgag ctgaggccgt gcgcctggaa cgcgcgcgcc cgggcgacta gagtcgcgta
      781 tggagcgccg agaccaggga gacgagcgag atggatgacg ccaagcgcac cgagatcgag
      841 gccgccgcgt tccgccggct ggtccagcat ttgcgcgagc gcaccgaggt ccagaacctg
      901 gacctgatgg agctcgccgg cttttgccgc aactgcctgt ccaagtggta ccgggctgag
      961 gccgagacgc gcggcgagga catcagctac gaggacgcgc gcgagatcgt ctacggcatg
     1021 ccctacgagt cgtggaaggc gaagtaccag gccgagcagc caaaagcgag gctcgacaac
     1081 ctcgcccacg accactgacg gctgctcgca agcggcgtcc tgcgctggtc agtcggccgg
     1141 cgcggggacc gcgacgccgg cctgggcgca ggcggcctgg agcgtgttcg acagcagcat
     1201 cgcaatcgtc atcggcccga caccgcccgg caccggggtg atcgcgcccg cgaccttgag
     1261 cgcctcggcg aaggcgacgt cgccgaccag ccgcgcgctg ccgtccgccg cggcgacgcg
     1321 gttgacgccg acgtcgatga cggcggcgcc gggcttgatc cagtcgcccc tgaccatctc
     1381 gggccggccc accgcggcga ccacgatgtc ggcgcggcgg cacaccgccg gcaggtcgcg
     1441 cgtccgcgaa tgggcgaggg tgacggtgca gttcgcggcg agcagcagca gcgccatcgg
     1501 cttgccgacg atggtcgagc ggccgatcac cagcgcctcg gcgccggcga gctcaccgag
     1561 cgcctcgtcg agcagcgcca tgcagccccg cggcgtgcac ggcaccagcg tcggccggcc
     1621 ggcgagcagc cgcccggcat tgagcggatg gaagccgtcg acgtccttgg ccggatcgac
     1681 cgcctcggtt accgcgccgg catcgacctg cggcggcagc ggcagctgga ccaggatgcc
     1741 gtgcaccgcg cggtcggcat tgagcgccgc gacctcggcc agcacgtcgc cggtcgaggc
     1801 ggaggcgtgc aggcgccggc tcacgctcgc catgccggcg gcctcggcgg cgcggccctt
     1861 gttgcgcaca tagacctcgc tcgccgcatc ctcgccgacc agcaccaccg caagcccggg
     1921 cgtgggcccg cccgcggcgg cgagcgcggc gacctggcgc gcggtgtggg cgcgcaggcg
     1981 cgcggccagc gccttgccgt cgatgatcct ggcctcggtc atggccgcgc ctggatagcg
     2041 caccgggcgg cgctgtcaac cgcgcccgat cggccggctg ccggtcccgg gccggcttgc
     2101 cggtcaaggg cctttcggat aggtagggat cgcgcgctcc tggggcgtag ccaagcggta
     2161 aggcaacggg ttttggtccc gtgaccgcag gttcgaatcc tgccgcccca gccacgccag
     2221 aggtgtttgg tcgatgcggc cggcggcgac catcgcgagg ccgccgggcg gaaggccggc
     2281 gtcagcgcat cggccgcagg cctcgccggc ggcggcgcgc cgggcgcgcg gcgctcacgc
     2341 ctccgactcg gtggtcgtga cctgctcggc ctggccctcc ttcttcttct cggccttttc
     2401 cgccttgacc ttggcgcggg cggccttctt ggcggccttg acccgctcgc gttccatacg
     2461 ttcgaaccgg taatttggct tgcgcggcat ggatgtcctt cctgttgatc cgatggtggc
     2521 catcatcacg cccgtccggg accccggtcc ggaccacgca atcatgacgc aagagcttga
     2581 ggcacggcac ggagccatga cggctgcgtc ggctgcgata gtggcaatcc ggtgccgatt
     2641 tgtcacggac tcatgccgcc ggcgccattg cgcggcaccg ggcgcgggac tacaagcggg
     2701 gacggcaggg ccggggaggg tggccatggg tgagctgttc gatgtcgcgc gccggcggct
     2761 cgacgaggcc gcgcagctta tcgacatcga ccccgacgtg ctggagaagc tccgttaccc
     2821 gaaagagacg ctggcggcga gcctggcggt gcgcatggac gacggcgcgc gcaagtcgtt
     2881 caaggcctgg cgctgccgct acgacgacac ccgcggcccg acaaagggcg gcatccgcta
     2941 tcacccggcg gtcaacatcg acgaggtgat ggcgctggcg ttctggatga ccttcaagtg
     3001 cgcggtggtg gacctgccgt tcggcggcgc caagggcggc gtcgcggtcg accccaagcg
     3061 cttgtcgcgc gccgagctcg agcgactgtc gcgcgccttc gcccatgcct tcgcaaactt
     3121 tatcggccca gagcgcgaca tccccgcgcc cgacgtctac accaacgcca tggtcatggg
     3181 ctggatcgcc gacgagtaca gctcgatcgt cggccggccg tcgccggcgg tggtcaccgg
     3241 caagccgatc gtgctcggcg gctcgcgcgg gcgcgacagc gccaccgggc gcggcggcta
     3301 tgtcacgctc cgccatctcg agcacgagtt cggcttcagg cccgagacca cgcgggtcgc
     3361 gatccagggc ttcggcaacg ccgccagcca ctgcgcgcgg ctgttgcacg gcgacggcta
     3421 caaggtggtc ggcgtgtccg attcgcgctc ggcgctgtac gacccggacg ggctcgaccc
     3481 ggccgcggtg ctcgaccaca agcggcgcac cggcgcggtc gccggcgcgc ccgggcgcag
     3541 caggccgcgc gagctgtcga accaggagct gctcgaggcc gagtgcgacg tgctgatccc
     3601 ggccgcgctc gagaaccaga tcaccgccgc caacgccgcc gcggtccagg cgcgcgtggt
     3661 gctcgagatc gccaacggcc cgaccacgcc ggacgccgac gccgagctcg cccggcgcgg
     3721 ggtggcgctg gtgcccgaca tcctggccaa tgccggcggg gtgaccgtgt cctatttcga
     3781 gtgggtccag aacaggtccg gcctgtactg gtccgaggac gaggtcgcca ggcggctggg
     3841 cgagttcatg ctgcccaacg tcgatgcggt ctggggcatt gcgcgcgagc gcgaggtgtc
     3901 gttgcgtacc gcggcctatg tgcacgcgct ggggcgcatc gccgaggcgg tcgccgccca
     3961 cggcaccaag gcctacttca tgtcctgaga tgtcctgata ccagttcgcg ctgatcttaa
     4021 cccatcgcca gcagcgtcat cgcgagaccg cgaagcggtc gtggcgatcc agaaccgcag
     4081 gcgacgcaac gagaattctg gatcgcttcg ctttgctcgc gatgctgggt ggacggcccc
     4141 ctgacggcat cgatgtgcca gactggggtc attgacccac cagcagagga gaccgtccag
     4201 atgcacgcta tcacgatcgg gttggatatc gcgaagtcgg tgtttcaggt ccacggcgag
     4261 gatgcgcagg gcaggattgt tctgcagaag cggctcaggc gctcgcaggt agtgccc
//
LOCUS       DASWHU010000021         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2593770, whole genome shotgun sequence.
ACCESSION   DASWHU010000021 DASWHU010000000
VERSION     DASWHU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593770"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1725
                     /locus_tag="VGB88_00725"
     CDS             289..1725
                     /locus_tag="VGB88_00725"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX9645988.1"
                     /translation="MTTLVPVLLAGGLGTRLWPLSREVYPKQLMALTGPESLLQQAAR
                     RALLCAPAERVVTVTTESHYFPVRDQLDAVDPRLTGHLLLEPAGRNTAAAVAVAALYA
                     ETLAKDATLFVAPADHVVRDADVVAGAVRLASGFPDHLVTFGIKPDRAETGYGYIECG
                     ERIDDKLAVNAVSSFIEKPDAETAQRLIDDRPVLWNSGMFVFRARLLIEELMRHAPAI
                     GAAAEAAFAARRAEAGAVRFPADRYAAIPAAPIDTAVMERSDKVAVVPIDPGWSDVGS
                     WLKLWQASERDAAGNAVVGDALVEDSRNCLVRSTTRLVACAGLDELVVTETADAVLVA
                     ALGADGAIRRIVERLREAGREEAVRHLEEMRPWGSFRVLLAGPRFKIKEIVVKPGARL
                     SLQAHQRRSEHWVVVAGTARITSGEWTGTIGPNRSAYVPVGARHRIENPGEEPLHIVE
                     VQCGDYVGEDDIVRFEDAYGREHMETTS"
     gene            1722..2243
                     /locus_tag="VGB88_00730"
     CDS             1722..2243
                     /locus_tag="VGB88_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9645989.1"
                     /translation="MTAVDWPLDLHRALAEAEVRLVGVVPDAGHARLIELCEADPDMR
                     MVALTTEEEGIGLAAGAWLGGVRAALLMQSSGVGNCVNAIASVSRACQFPLLMLVTMR
                     GEWGEGNPWQVPMGRATPRVLDDLGVQVARADRPEDAAPALEAMAGLAFKSQMACAVL
                     LSQRLIGAKSFQD"
     gene            2403..2993
                     /locus_tag="VGB88_00735"
     CDS             2403..2993
                     /locus_tag="VGB88_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9645990.1"
                     /translation="MPRNATTDNALDRRSVVADLLAGRGELLVVCGLGGTTWDVMSAG
                     DNPLNFYSWGGMGSAAMTGLGLALARPERTVPVVTGDGEMLMGMGSLATIAAQAPENL
                     AVAVIDNELYGETGRQPTHTALGADLALIAKGCGIARAETISDAAGVAAFRARVHGQP
                     GPHFAAIKVGRAEHAMVLPERDGTAIKTRFRRALLG"
     gene            3078..>3412
                     /locus_tag="VGB88_00740"
     CDS             3078..>3412
                     /locus_tag="VGB88_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9645991.1"
                     /translation="MQRFQMYIDGKFSDAASGEWFETQNPYTGKTWALVARGGAPDAE
                     RAVEAAHRAFTSPDWRKLTATQRGALLRKLGDLVAERAEEFAEYEVRDNGKLIAEMAV
                     QLRYVPQWY"
BASE COUNT          492 a         1215 c         1237 g          468 t
ORIGIN      
        1 aggtcgcctt cgacggcctg cctgcgcgaa gccgaagcgg cgtcgcttcg gcgaaggcag
       61 gcggcggcgc gaccctgatc gacgcgcgca tcgacgccgg cggctaccgc gcgatgttcg
      121 aggcgatccg cggcgcaccc ggcgcggcgt cgaacacatg actcgagtgc ccgtcgacca
      181 gccttgtttg agtcacgcgg agacgcggag gacatggagg attcgtgatc gagtccgccg
      241 cgccttccgc gcctccgcgt gccccaaact gccttcgccc ggcggccgat gaccacgctg
      301 gtccccgtgc tgctcgccgg cggcctgggc acgcgcctgt ggccgctcag ccgcgaggtc
      361 tatcccaagc agctgatggc gctgaccggg cctgagtcgc tgttgcagca ggcggcgcgc
      421 cgcgcgctgt tgtgcgcgcc ggccgagcgg gtggtgacgg tgaccaccga gagccactac
      481 ttcccggtcc gcgaccagct cgacgcggtc gacccgaggc tcaccgggca cctgctgctc
      541 gagcccgcgg gccgcaacac cgccgccgcg gtggcggtcg cggccctgta cgccgagacg
      601 ctcgccaagg atgcgacgct gttcgtcgcc cccgccgacc acgtggtccg cgacgccgac
      661 gtggtggccg gcgcggtgcg cctggcatcc gggttcccgg accacctggt gaccttcggc
      721 atcaagcccg accgcgccga gaccggctac ggctacatcg aatgcggcga gcggatcgac
      781 gacaagctgg ccgtgaacgc ggtgtcgtcg ttcatcgaga agcccgacgc cgagaccgcg
      841 caacgcctga tcgatgatcg gccggtgctg tggaacagcg gcatgttcgt gttccgcgcg
      901 aggctgctga tcgaagaact catgcgccat gcacccgcga tcggcgccgc cgccgaggcg
      961 gcgttcgcgg cgcggcgcgc agaggccggc gcggtgcgtt tcccggccga ccgttacgcc
     1021 gcgatcccgg ccgcgccgat cgacaccgcg gtgatggagc gctcggacaa ggtggcggtg
     1081 gtgccgatcg accccggctg gtcggacgtc ggctcgtggc tcaagctgtg gcaggcgagc
     1141 gagcgcgacg cggccggcaa cgccgtggtc ggcgacgccc tggtcgagga cagccgaaat
     1201 tgcctggtgc gcagcaccac gcgcctggtc gcctgcgccg ggctcgacga gctggtggtg
     1261 accgagaccg cggatgcggt cctcgtcgcc gcgctcggcg ccgacggcgc catccgccgg
     1321 atcgtcgagc ggctgcgcga ggccgggcgc gaggaggcgg tgcgccacct cgaggagatg
     1381 cgcccgtggg gctcgttccg ggtgctgctt gccgggccgc ggttcaagat caaggagatc
     1441 gtggtcaagc ccggcgcccg gctcagcctc caggcgcacc agcgccgcag cgagcactgg
     1501 gtggtggtcg ccggcaccgc ccgcatcacc tcgggcgagt ggaccggcac catcggcccc
     1561 aaccgctcgg cctatgtgcc ggtcggcgcg cgccaccgga tcgagaaccc cggagaagag
     1621 ccgttgcaca tcgtcgaggt ccagtgcggc gactatgtcg gcgaggacga catcgtccgc
     1681 ttcgaggacg cttacgggcg cgagcatatg gagacaacgt cgtgactgcg gtggactggc
     1741 cgcttgacct gcaccgcgcg ctcgccgagg ccgaggtgcg cctggtcggt gtggtgcccg
     1801 acgccgggca cgcgcgcctg atcgagctgt gcgaggccga cccggacatg cggatggtgg
     1861 cactgaccac cgaggaggaa ggcatcggcc ttgccgccgg cgcctggctc ggcggcgtgc
     1921 gcgcggcgct gctgatgcag tcgagcgggg tcggcaactg cgtcaacgcc atcgcctcgg
     1981 tcagccgcgc ctgccagttc ccgctgctga tgctggtgac catgcgcggc gagtggggcg
     2041 agggcaaccc gtggcaggtg ccgatgggcc gcgccacccc gcgcgtgctc gacgacctcg
     2101 gcgtccaggt cgcgcgcgcc gaccggcccg aggacgctgc gcccgcgctc gaggcgatgg
     2161 cggggctcgc cttcaagagc cagatggcgt gcgcggtgct tctgtcgcag cgcctgatcg
     2221 gcgccaagag cttccaggac tgaggaccta aatgagtccg ttatcgtcct tcgagacgct
     2281 cgctgcgctc gctcctcagg acgaggaggt tttactcact aatgatttgc ctcaccctga
     2341 ggagcgcccg aagggcgcgt ctcgaagggt gtgccgaggg agtcgggaac gctgagcgcg
     2401 ccatgccgag aaacgccacc acggacaacg cactcgaccg ccgttcggtg gtcgccgatc
     2461 tgctcgccgg gcgcggcgag ctgctggtcg tgtgcggcct cggcggcacc acctgggacg
     2521 tgatgagcgc cggcgacaac ccgctcaact tctacagctg gggcggcatg ggcagcgcgg
     2581 cgatgaccgg cctcgggctc gcgctggcgc ggcctgagcg caccgtgccg gtggtcaccg
     2641 gcgacggcga gatgctgatg ggcatgggct cgctcgccac catcgccgcg caggcgccgg
     2701 agaacctcgc ggtcgccgtg atcgacaacg agctctacgg cgagaccggc cgccagccga
     2761 cccacaccgc gctcggcgcc gacctcgcct tgatcgccaa gggctgcggc atcgcgcgcg
     2821 cggaaaccat ctccgatgcc gccggcgtcg ccgcgttccg cgcccgcgtc cacggccagc
     2881 ccggccccca cttcgccgcg atcaaggtcg ggcgcgcgga gcacgcgatg gtgctgcccg
     2941 agcgcgacgg caccgcgatc aagacccgct tccgccgcgc gctgctcggc tgacgcagtc
     3001 ccgcggcttt acctgagccg ccgccggcgg gacaatcgtc cacaagttag gccgacacga
     3061 gagagggagg ccacgccatg caacggttcc agatgtacat cgacggcaag ttctccgacg
     3121 ccgcatccgg cgagtggttc gagacccaga acccctatac cggcaagacc tgggcgctgg
     3181 tcgcgcgcgg cggtgcgccc gacgcagagc gcgcggtcga ggcggcgcac cgggccttca
     3241 cctctcccga ctggcgcaag ctcaccgcga cccagcgcgg cgcgctgctg cgcaagctcg
     3301 gcgacctggt tgccgagcgc gccgaggagt tcgccgagta cgaggtgcgc gacaacggca
     3361 agctgatcgc cgagatggcg gtgcagctcc gctacgtgcc gcagtggtac ta
//
LOCUS       DASWHU010000022        10293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2593786, whole genome shotgun sequence.
ACCESSION   DASWHU010000022 DASWHU010000000
VERSION     DASWHU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593786"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1307)
                     /locus_tag="VGB88_00745"
     CDS             complement(3..1307)
                     /locus_tag="VGB88_00745"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9645992.1"
                     /translation="MAAQRIVIVGGGAGGLELAIRLGDSLGRRERAEVTLVDTSLTHL
                     WKPLLHEVAAGTLDSHEDAIEFLAQARAHRFRFRLGRLEGLDRGAKQVRLGAITDRDG
                     IELAPARSLAYDILVIAVGGTVNDFGVPGAKEHCTRLDGQEEAERFHRAFLGLHLHGQ
                     AADGFAQAGWLGVGIVGAGATGVELAAELHAASRQLATYGLGGGGQARDLRLVLIEAA
                     GRVLPGLPERVSRATAARLGEIGVELRTGEQVTRVTAEAIETTAGVLPCRLKVWAAGV
                     KAPDFLATLDGLEANRANQLLVRPTLQTTADDAVFALGDCAACPWPGHERTVPPRAQA
                     AHQQAALLARSLARRLDGRPLAAFVYKDRGSLVSLSRYTTLGNLMGNLLGEVTFEGWL
                     ARLAYRSLYRMHQRAVYGLPRMVLILLADWFRRGVGPALKLH"
     gene            complement(1367..1777)
                     /locus_tag="VGB88_00750"
     CDS             complement(1367..1777)
                     /locus_tag="VGB88_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX9645993.1"
                     /translation="MESETALTADLHWLVYSTLLLLVLWIPYIVAASQARGLTRAMGY
                     PTGNYADLPDWAQRAQRAHMNMVENLAPFAALVLVAHLSGAANATTAAAAQVFFWARL
                     VHAGILIAGIPWLRTIVWTIGWIACLVILYEILV"
     gene            complement(1898..2272)
                     /locus_tag="VGB88_00755"
     CDS             complement(1898..2272)
                     /locus_tag="VGB88_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9645994.1"
                     /translation="MIAVDTSTLIAYLADDAGRDVDALDLALAQREVCLPPAVVTEIL
                     SAPGDSEGLARLILALPALEISDGFWERAGRVRRELRARKLRAPLADALICQSCLDHG
                     VPLLTRDGDFQAFATHCGLQLA"
     gene            complement(2269..2475)
                     /locus_tag="VGB88_00760"
     CDS             complement(2269..2475)
                     /locus_tag="VGB88_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645995.1"
                     /translation="MSRTRKITAHVPVELLQRAQEATGKGVTETVRRGLDLLVAAEAA
                     DELRRLRGKVRLDIDLAELRRDRR"
     gene            complement(2539..2976)
                     /locus_tag="VGB88_00765"
     CDS             complement(2539..2976)
                     /locus_tag="VGB88_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX9645996.1"
                     /translation="MKRLHVSLSVDDLAASIRFYSGLFGSPPTVLKPDYAKWLLDDPR
                     VNLSLVARGRIPGLDHLGIQAETPAELAELAGRLEAAGLAVQDRSAVTCCYARSDKAW
                     ARDPQGIRWEAFHTFAASDSFDDPAGAPCCPPKPEIETTAPCC"
     gene            complement(2957..3328)
                     /locus_tag="VGB88_00770"
     CDS             complement(2957..3328)
                     /locus_tag="VGB88_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9645997.1"
                     /translation="MEIQSAITALAALAQDSRLRVFRLLVAHGRAGLAAGEIARSLGV
                     PHNTMSAHLGVLARAGLVRSRRDGRSVIYAADFAGVRGLLGFLMEDCCRARAELCAPL
                     LDAALPDPDLMEDCCDEAPAR"
     gene            complement(3381..3590)
                     /locus_tag="VGB88_00775"
     CDS             complement(3381..3590)
                     /locus_tag="VGB88_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645998.1"
                     /translation="MAAFLFFGARTGRGLAGDGRSGPGSTAEVPVRGRSSEIEAGTRR
                     IAPARIWLFLRRNHILVQCNRGVQA"
     gene            3589..3876
                     /locus_tag="VGB88_00780"
     CDS             3589..3876
                     /locus_tag="VGB88_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9645999.1"
                     /translation="MRKVWGLGLATGLALAAAPAEAGCAPREVVVKALERDYGEYATF
                     VGVQRDGGSLIEVFVSDDGSWTVIESFADGRACSRAFGESWVHLPKAGPDA"
     gene            3955..4734
                     /locus_tag="VGB88_00785"
     CDS             3955..4734
                     /locus_tag="VGB88_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9646000.1"
                     /translation="MDLGIAGRKAIVCASSKGLGRACALALAREGVAVTINARDPEAL
                     EKTAAEIRSQTGAEVTAVAADVTTAAGQAKLLAACPAPDILVNNAGGPPPGDFRDWSR
                     ADWITAVVANMLTPIELIKATVDGMIGRRFGRIVNITSAATKNPIPDLGMSNGARTGL
                     AGFVAGLARQTVRHNVTINCLLPGRFVTDRMRQHVGFGARAAGQDYDSYAKALAAQEP
                     AGRFGDPAEFGDACAFLCSAQMGYVTGQHFLMDGGNFRGTF"
     gene            complement(4741..6807)
                     /locus_tag="VGB88_00790"
     CDS             complement(4741..6807)
                     /locus_tag="VGB88_00790"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9646001.1"
                     /translation="MMDTARAPERAAASEATGGALVVRLIDRLSALTGALAVAGLLVM
                     TSIVCFEVFSRYVLNAPTYWVTEIATYILVGMTFIALAAAHVSGSHIRVELLLGLLRP
                     APRASVELVATWLGLAFVAFAAWQMIAFNHQEYVHDTRDWGLLATPQWLPELPVSVGL
                     VAFALSILADIRRARPPASARAHWTVPVLGVALALTLVALGPLPVAVAGTRLDWGSIA
                     IAAALAGAVLAWSGPRIALAVCALIAALAVAFWAARGWPLLPVASLLVAALLVLLLIG
                     VRVALALGTVGALGLYFLLPQPQLSVLAERSWNSVNSFTLTAVPMYVLMGGLLLRSGA
                     SSDMFDALMRWFGRLPGGIAHASVGASTIFAAVSGSSLATAATMGTIACPEMVRRGYS
                     ARLSYGVVAGGATLGILIPPSIAMIIYGTTVGAPVTVLFIAGIVPGLALMLAFMVGVL
                     VWSVVQPGAAPRGDAYAWRDKLAALGGVAPFALLIVVVLGSLYAGIATPTESGGVGAA
                     VALVLCALRRRLSWTLLRETAAETVRVTAFLLLIIVGASILSWVFDYLRLSASLVEEV
                     EAAGLAPWTVMLAIGLVYVALGMFIDPISMMLMTLPVTFPIVTALGFDAVWFGIALVL
                     MIEVALITPPVGMVLFVLRGLSGEVALGEIVAGAVPFVAIILAFLVVIYLFPGIVAWL
                     PGRMAP"
     gene            7023..7652
                     /locus_tag="VGB88_00795"
     CDS             7023..7652
                     /locus_tag="VGB88_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9646002.1"
                     /translation="MTLRSDDPPGVPAAHAPEVVDLEERTWLVRHCRGDSSAFPALIA
                     AYRRPVYSYLVRTGVAEADRDDVFQTIFLNCHAAAASYDSSRPLAPWLFTVVANAVRS
                     HFRARPTHLAVVAADPPADAPDPDPGPERIAEGRESVARLEAALDSLPPAQRQVLLLV
                     TIAGLRQQDVAACLKLPLNTVKTHLRRARLALAAGLARAGGPGESDDHL"
     gene            7639..10140
                     /locus_tag="VGB88_00800"
     CDS             7639..10140
                     /locus_tag="VGB88_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646003.1"
                     /translation="MTICDAIQKRLAEDGTAVADRDAEVRRHIDGCADCRRVLAELGR
                     LDTALAALPVYDAPDALVAATLDAVRRAPGAASAPARPRVLRGYLAGGLAASVVALGL
                     LGVTYSVLGPTWQRSPVVTELADGNSTVEAPAATATGDDVFAVGPQFQRAAPEREAYD
                     RDGPELAELADERREQGAYRTLDDREWDVIAQLDEQAAAGAREQRQAAGQNAEDAGER
                     RQLEESLTSHAMAKAAPAKPDSEPAENRAAARSVDRLGADEGARMRAVPSAPPEAAER
                     KDRADERQEMSAAAGASVRAAVVGGEIGEPADPRRRARDFLAGWRAQDELLVQPARGY
                     WANTYIPGDPEMRLLAARLRDWNRAALGRDTRLEQLVRPVGQPFDAPRDAAMAVYLDA
                     DAAAIAGPTRLRVQVGLKGVERQGGHRPAMTIGVVVDLRDAVDPGKGARIRALIAALD
                     RARQPEDSFSLTVAGPAGGLLVPPEQFRHGPLQVAMARMFDPSQRSASSALDLAQALA
                     LAEEAVRQGDDPDAVLGSSLVLLVTDSALAGEAAALEPLVHRNAVDGVALSVVGLGGQ
                     GDLAPIERLVAAGQGNRRILDSAQAADGLVDRELHAASRSVARALRLRVRLAPGVELI
                     DILGSRPLGEPQAQRVREAEQAIDRRLARNLGIEADRGRDEEGIQIVIPNFYAGDTHV
                     ILLDVLADRPGPIADVTLRYKDVVYLRNGVAGASLAIDDGEAAPGPLQANVAKNLVAW
                     EFARDMREVGRMLAAGEVQAAVARIADLRGLIAGLRLEVPGWSGDPDLAADESLLDAY
                     LAVLQSPAAGDAVQRDYLADSLHYAAFRKLQAAAR"
BASE COUNT         1572 a         3713 c         3748 g         1260 t
ORIGIN      
        1 cctcagtgca gcttgagcgc ggggccgacg ccgcggcgga accagtcggc gagcaggatc
       61 agcaccatgc gcggcagccc gtacaccgcg cgctggtgca tgcggtagag cgagcggtag
      121 gcaagccgcg cgagccagcc ctcgaaggtg acctcgccca gcaggttgcc catcaggttg
      181 ccgagcgtcg tgtagcggct gagcgagacc agcgagccgc ggtccttgta gacgaatgcg
      241 gccagcggcc gcccgtccag ccggcgcgcg agcgacctcg cgagcagcgc cgcctgctgg
      301 tgcgcggcct gggcgcgcgg gggcacggtc cgctcgtgcc ccggccacgg gcaggcggcg
      361 cagtcgccga gcgcgaacac ggcgtcgtcg gcggtggtct gaagtgtcgg gcgcaccaga
      421 agctgattgg cgcggttggc ctcgaggccg tcgagggtgg cgaggaagtc cggcgccttg
      481 actcccgcgg cccacacctt gagccggcat ggcagcaccc cggccgtggt ctcgatcgcc
      541 tcagcggtca cccgcgtgac ctgctcgccg gtgcgcagct cgaccccgat ctcgccgaga
      601 cgcgccgcgg ttgcgcgcga gacccgctcg ggcaagcccg gcagcacgcg cccggccgcc
      661 tcgatcagca ccagacgcag gtcgcgcgct tggccgccgc cgccgaggcc gtaagtggcc
      721 agctggcggc tcgccgcgtg cagctcggcg gcgagctcga ccccggtggc gccggcgccg
      781 acgatgccga ccccgagcca gcctgcctgc gcgaagccgt ctgccgcctg gccgtgcaga
      841 tgcaggccga ggaaggcgcg gtggaagcgc tcggcctcct cctggccgtc gaggcgggtg
      901 cagtgctcct tcgccccggg cacgccgaag tcattcaccg tgccgccgac cgcgatcacc
      961 aggatgtcgt aggcgaggct gcgcgccggc gcgagctcga tcccgtcgcg gtcggtgatg
     1021 gcgccgagcc gaacctgctt ggccccgcgg tcgagcccct ccaggcggcc gagccggaac
     1081 cggaagcgat gggcgcgcgc ctgggccagg aactcgatcg cgtcctcgtg ggaatcgagc
     1141 gtgccggcgg cgacctcgtg cagcagcggc ttccacagat gggtgagcga ggtgtcgacc
     1201 agggtgacct cggcccgctc ccgccgcccg agcgagtcgc cgaggcggat ggcgagctcg
     1261 agcccgccgg cgccgccgcc gacgatgacg atgcgctgcg cggccatgcc cgatccccct
     1321 ccgccgccgg caccggcccc cgacgccggc ggccgggggg tcgggctcag acgaggattt
     1381 cgtacaggat caccaggcag gcgatccagc cgatcgtcca cacgatggtg cgcagccagg
     1441 gtatgccggc gatgaggatg cccgcatgga ccaggcgcgc ccagaagaac acctgcgccg
     1501 cggcggcggt ggtcgcattg gccgcgccgg aaaggtgggc gacgaggacc agcgccgcga
     1561 acggcgccag gttctcgacc atgttcatgt gcgcgcgctg ggcgcgctgc gcccagtcgg
     1621 gtaggtcggc gtagttgccg gtcggataac ccatcgcgcg cgtcagcccg cgcgcctgcg
     1681 acgccgcgac gatgtagggt atccaaagca ccagcagtaa cagcgtgctg tagaccagcc
     1741 aatgcaggtc ggcagtcagc gcggtctcgc tctccatgtc acccactcca ttgacgccgg
     1801 cgcgaacccc cgccgcgccg attcaaataa tggtagcacc agtgggctac caatacgaca
     1861 tttggggaga ctcggtattc attggcgcgt gttgggttca ggcgagctga aggccgcaat
     1921 gcgtcgcgaa ggcttggaaa tcgccgtcgc gcgtgagcaa tgggacgccg tgatcgagac
     1981 acgactgaca gatcagggcg tcggcaaggg gggcacggag cttgcgcgcc cgcaactcgc
     2041 gtcggacgcg acccgcacgc tcccagaacc cgtccgaaat ctcgagcgcc ggcagcgcga
     2101 ggatcaggcg cgccaggcct tcgctgtcgc ccggcgcgct gaggatttcg gtcacgaccg
     2161 ccggcggcag gcagacctcc ctctgcgcaa gcgcgaggtc gagcgcgtcc acgtcacggc
     2221 ccgcatcgtc agcgaggtac gcgatcagag tgctggtatc gaccgcaatc accggcggtc
     2281 gcggcgcagc tccgcaaggt cgatgtccaa gcgcaccttg ccgcgcaggc gacgcagttc
     2341 gtcagcggcc tccgccgcga cgagcaggtc gagtccgcgc cgcaccgttt cagtgacgcc
     2401 cttgcccgtc gcctcctgtg cgcgctggag cagctcgacg ggaacgtgcg ccgtaatttt
     2461 tcgcgtccga ctcatacggt accgtatacc gtaaattcgc gccgggatca aactccggca
     2521 agggaacggc cgcgccggtc agcagcacgg ggcggtcgtc tcgatttcgg gcttgggcgg
     2581 gcagcagggg gcgccggccg ggtcgtcgaa gctgtcgctg gcggcgaagg tgtggaaggc
     2641 ctcccagcgg atgccttggg ggtcgcgcgc ccacgccttg tcggacctgg cgtagcagca
     2701 ggtgaccgcg ctccggtcct gcactgccag gccggcggcc tcgagccggc cggcaagctc
     2761 ggcgagctcc gcgggagtct ccgcctggat gcccagatgg tcgaggccgg ggatgcgccc
     2821 gcgcgcgacc agcgacaggt tgaccctcgg gtcgtcaagc agccacttgg cgtagtcggg
     2881 cttcagcacc gtcggcgggc tcccgaacag gccggagtag aatcggatcg aggcggcgag
     2941 atcatcgacc gaaaggctaa cgtgcaggcg cttcatcgca gcagtcctcc atgaggtccg
     3001 ggtcgggcag ggcggcgtcg agcagcggcg cgcacagctc ggcacgcgcc cggcagcagt
     3061 cctccatcag gaatccgagc aggccgcgca cgccggcgaa gtcggcagcg tagatgaccg
     3121 agcggccgtc gcgccggctg cggaccaggc cggcgcgggc gagcacgccg agatgcgccg
     3181 acatggtgtt gtgcggcacg ccaaggctgc gcgcgatctc gcccgcggcc aggccggcgc
     3241 ggccgtgcgc caccagcagc cggaacacgc gcaaccggct gtcctgggcg agcgccgcga
     3301 gcgcggtgat ggcggattga atttccatat gtctggatat atcgacattt gaggcgaacg
     3361 tcaaacgtga cgaccgcttg tcaagcctgg actcctctgt tgcactgcac caaaatatgg
     3421 ttgcggcgca aaaagagcca gatccgggcc ggcgcgatgc gacgcgtgcc cgcctcgatt
     3481 tcggaggagc gcccgcggac gggcacttcg gcggtcgaac cggggcctga ccggccatcg
     3541 ccggccaatc cgcgccccgt gcgcgcgccg aaaaagagga acgcggccat gcgaaaggtt
     3601 tggggtctgg gtctcgccac aggattggct ctggcggcgg cacccgccga ggcggggtgc
     3661 gcgccccgcg aggtagtggt caaggcgctc gagcgcgact acggcgagta cgccaccttc
     3721 gtcggcgtgc agagggacgg cggctcgctg atcgaggtct tcgtctccga cgacggcagc
     3781 tggacggtga tcgagagctt cgccgatggc cgcgcctgca gccgcgcctt cggcgaaagc
     3841 tgggtgcacc tgcccaaggc cgggccggac gcctgatccg gcgcccgcgg cttcctaccg
     3901 gcaccggccg atgctagaca ggggccggcc gcacggcatc agggaggggg acgggtggat
     3961 ctcggcatcg ccggccgcaa ggccatcgtc tgcgcatcga gcaagggcct gggacgcgcc
     4021 tgcgcgctgg cgctcgcgcg cgaaggggtg gcggtgacca tcaacgcgcg cgaccccgag
     4081 gcgctggaga agaccgccgc cgagatccgc agccaaaccg gagccgaggt gaccgcggtc
     4141 gccgccgacg tgaccacggc ggcgggccag gccaagctgc tcgccgcctg cccagcgcca
     4201 gacatcctgg tcaacaacgc cggcgggccg ccgcccggcg acttccgcga ctggtcgcgc
     4261 gcggactgga tcacggccgt ggtcgccaac atgctgaccc cgatcgagct gatcaaggca
     4321 accgtcgacg gcatgatcgg gcgccgcttc gggcgcatcg tcaacatcac ctcggcagcg
     4381 accaagaacc cgatccccga cctcggcatg tcgaacggcg cgcgcaccgg gcttgccggc
     4441 ttcgtcgccg gcctcgcccg ccagacggtg cgccacaacg tcaccatcaa ctgcctgctg
     4501 ccgggccggt tcgtcaccga ccgcatgcgc cagcatgtcg gcttcggcgc gcgcgcggcg
     4561 ggccaggact acgacagcta cgccaaggcg ctggccgcgc aggagccggc cgggcggttc
     4621 ggcgatccgg cggagttcgg cgacgcctgc gcgttcctgt gctcggcgca gatgggctac
     4681 gtcaccggcc agcacttcct gatggacggc ggcaacttcc gcggcacgtt ctgagccggc
     4741 tcacggagcc atccggcccg gcagccaggc gacgatgccg gggaacaggt agatcaccac
     4801 caggaaggcg aggatgatgg cgacgaacgg caccgcgccg gcgacgatct cgccgagcgc
     4861 gacctcgccc gaaaggccgc gcagcacgaa cagcaccatg cccaccggcg gggtgatcag
     4921 cgccacctcg atcatcagca ccagcgcgat gccgaaccac accgcgtcga agccgagcgc
     4981 ggtgacgatc gggaaggtca ccggcaaggt catcagcatc atcgagatcg ggtcgatgaa
     5041 catgccgagc gcgacgtaga ccagcccgat cgccagcatc accgtccacg gcgcgagccc
     5101 cgcggcctcg acctcctcga ccaggctcgc ggacaggcgc aggtagtcga acacccagct
     5161 caggatcgac gcgccgacga tgatcagcag caggaacgcg gtcaccctga ccgtctcggc
     5221 cgcggtctcg cgcagcaagg tccagctcag cctgcggcgc agcgcgcaca gcaccagcgc
     5281 cacggcggcg ccgaccccgc cggactcggt cggcgtggcg atgccggcgt agagcgagcc
     5341 gagcaccacg acgatcagca gcgcgaacgg cgccaccccg cccagcgccg cgagcttgtc
     5401 gcgccacgcg taggcatcgc cccgcggcgc cgcccccggc tggaccaccg accacaccag
     5461 cacgccgacc atgaacgcga gcatcagcgc gagccccggc acgatgccgg cgatgaacag
     5521 cacggtgacc ggcgcgccga ccgtggtgcc gtagatgatc atggcgatgc tgggcgggat
     5581 caggatgccg agggtggcgc cgccggcgac gacgccgtag ctgagccgcg cgctgtagcc
     5641 gcgccggacc atctcggggc aggcgatggt gcccatggtc gccgccgtcg ccaggctcga
     5701 gccggagacc gcggcgaaga tggtcgaggc gccgacgctg gcatgggcga tgccgccggg
     5761 cagccgcccg aaccagcgca tcagagcatc gaacatgtcg ctgctcgcgc cgctgcgcag
     5821 cagcagcccg cccatcagca cgtacatcgg caccgcggtc agcgtgaacg agttgacgct
     5881 gttccacgac cgctcggcga gcaccgaaag ctgcggctgc ggcagcagga aatagagccc
     5941 gagcgcaccg acggtgccga gcgcgagcgc gacccgcacc ccgatcagca gcagcaccag
     6001 cagcgccgcg accagcaggc tcgcgaccgg cagcagcggc cagccgcgcg ccgcccagaa
     6061 agcgacggcg agcgcggcga tgagcgcgca cacggcgagc gcgatgcgcg gcccgctcca
     6121 ggcgagcacg gcgcccgcca gcgccgcagc gatcgcgatc gagccccagt ccaggcgcgt
     6181 gccggcgacc gcgaccggca gcggcccgag cgcaaccagg gtgagcgcga gcgcgacgcc
     6241 gaggaccggc accgtccagt gcgccctggc gctcgccggc gggcgcgcgc gccggatgtc
     6301 ggcgaggatc gagagcgcga acgcgaccag gccgaccgag accggcagct cggggagcca
     6361 ttgcggtgtc gccagcagcc cccagtcgcg cgtgtcgtga acgtattcct ggtggttgaa
     6421 cgcgatcatc tgccaggcgg cgaaggcgac gaaggcgagg ccgagccagg tcgcgaccag
     6481 ctcgacgctc gcccgcggcg cggggcgcag cagaccgagc aagagctcga ccctgatgtg
     6541 gctgcccgat acgtgcgccg cggccagcgc gatgaaggtc atgccgacca ggatgtaggt
     6601 ggcgatctcg gtcacccagt aggtcggcgc gttcagcacg tagcgcgaga acacctcgaa
     6661 gcagacgatc gaggtcatga ccagcaggcc ggcgacggcg agagcgccgg tgagcgcgct
     6721 cagccgatcg atcaggcgga ccaccagcgc accgccggtc gcctccgagg ccgcggcccg
     6781 ctcgggcgcc cgcgccgtat ccatcatcgc cccccacgcc ccgcccggcg tcgccgcgga
     6841 cggtgccgag actaggcgat cggccggcaa agctgtagcg atgcgtggct ccccatcccc
     6901 ggcgcggcgg acaccgcggc aaaaaaatgc gctccccgcg ccggcgcgtg aaacctttga
     6961 cgggctcccg cgtacggggg atcagtaggc gatcccggag cgcgtgaaag gtgcatggac
     7021 tggtgactct ccgatcggac gatccgcccg gcgtcccggc cgcgcacgcg ccggaggtgg
     7081 tggatctcga ggagcgaaca tggctggtgc gccactgccg gggcgactcg agcgcgtttc
     7141 ccgccctgat cgcggcctat cgacggcccg tctacagcta tctggtgcgg accggcgtgg
     7201 ccgaagcgga tcgtgacgac gtgtttcaga ccatcttcct caactgccat gccgcggccg
     7261 cgagctacga ttccagccgg ccgctggcgc cgtggctgtt caccgtcgtc gccaacgcgg
     7321 tgcgcagcca ttttcgcgcc cggcctacgc acctcgcggt tgtcgccgcg gacccgccgg
     7381 cggatgcgcc cgaccccgat cccggccccg agcgcatcgc cgaggggcgc gagtccgtcg
     7441 cccgcctgga ggcggcgctc gactccttgc cgccggcgca gcgccaggtg ctgctgctgg
     7501 tgacgatcgc aggcctccgc cagcaggacg tggccgcgtg cctcaagctg ccgctgaaca
     7561 ccgtcaagac gcatctgcgc cgcgcccgcc tggcgctggc ggcgggcctg gcacgggccg
     7621 gcggcccggg agagagcgat gaccatctgt gacgccatcc aaaagcggct ggccgaggac
     7681 ggcaccgcgg tcgcggaccg cgacgccgag gtccgccgcc atatcgacgg ctgcgccgac
     7741 tgccggcggg tgctggccga gctcggccgg ctcgatacgg cgctcgccgc gctgcccgtg
     7801 tacgatgcgc cggacgccct ggtggcggcg accctcgacg ccgtccgtcg ggcgccaggc
     7861 gccgcctcgg cgccggcgcg gccgcgcgtc ctgcgcggat atctcgccgg cggcctggcc
     7921 gcgtcggtgg tcgcgctcgg cctgctcggc gtgacctaca gcgtgctcgg cccgacctgg
     7981 cagcggtcgc cggtggtgac cgagctcgcc gacggcaaca gcaccgtcga ggcgcccgcg
     8041 gcaaccgcga ccggcgacga cgtcttcgcc gtcggtccgc aattccagcg cgcggcgccg
     8101 gagcgcgagg cctacgaccg cgacggtcct gagttggccg agcttgccga cgagcggcgc
     8161 gagcagggcg cgtaccggac tctcgacgac cgcgaatggg acgtcatcgc gcagctcgac
     8221 gagcaggcgg cggcgggcgc gcgcgagcaa cggcaagcgg ccgggcaaaa cgccgaggac
     8281 gcaggcgagc gccggcagct cgaagagtca ctgacgagcc acgcgatggc caaggccgcg
     8341 ccggccaagc ccgacagcga gccggcggag aaccgggcgg cagcaaggtc tgtggaccgg
     8401 ctcggcgcgg acgaaggcgc caggatgcgg gcggtgccgt ccgcgccgcc ggaggccgcc
     8461 gagcgcaagg accgggccga cgagcggcag gagatgagcg ccgcggccgg tgcgtccgtg
     8521 cgcgcggccg tggtcggcgg ggagattggc gagccggcgg acccgcgccg ccgcgcccgg
     8581 gacttcctcg ccggctggcg cgcgcaggac gaactcttgg tccagccggc gcgcggctac
     8641 tgggccaaca cctatatccc gggcgatccg gagatgcggc tgctggcggc gcgcctgcgc
     8701 gactggaacc gcgcggccct cgggcgggac acccgcctgg aacagctggt gcggccggtg
     8761 ggtcagccgt tcgatgcccc gcgcgatgcg gccatggccg tctatctgga cgcggatgcg
     8821 gcggcgatcg ccggccccac ccggctgcgc gtccaggtcg gcctcaaggg cgtcgagcgc
     8881 cagggcgggc atcgcccggc gatgaccatc ggcgtggtcg tcgatctcag ggacgccgtc
     8941 gaccccggca agggcgcgcg catccgcgcc ctgatcgcgg cgctggaccg cgcccgccag
     9001 ccggaggaca gcttctcgct caccgtcgcc ggccccgccg gcgggctgct ggtgccgccc
     9061 gagcagttcc ggcacggccc cttgcaagtg gcgatggcgc gcatgttcga cccgtcgcag
     9121 cggtccgcat cgtccgcgct cgacctggcg caggcgctcg cgctcgccga ggaggcggtg
     9181 cgccaaggcg acgaccccga cgccgtcctt ggctcgagcc tggtgctcct ggtcactgat
     9241 tccgcgctcg cgggcgaggc cgccgcgctc gagccgctgg tccaccgcaa cgccgtcgac
     9301 ggcgtcgcgt tgagcgtcgt cggcctcggc gggcagggcg acctcgcgcc gatcgagcgg
     9361 ctggtcgcgg ccggccaggg caaccggcgg attctcgact cggcgcaggc ggccgacggc
     9421 ctggtcgacc gcgagctgca cgcggccagc cgctcggtcg cgcgcgcgct gaggctgcgg
     9481 gtgcgcctcg cccccggcgt cgagctgatc gacatcctgg gctcccggcc cttgggcgag
     9541 ccgcaggccc agcgcgtgcg cgaggccgag caggcgatcg accggcggct ggcgcgcaat
     9601 ctcggcatcg aggccgaccg cggccgggac gaggaaggca tccagatcgt catccccaac
     9661 ttctacgccg gcgatacgca cgtgatcctg ctcgacgtgc tcgccgaccg gccggggccg
     9721 atcgccgacg tgacgctgcg ctacaaggac gtggtctatc tgcgcaacgg cgtggccggc
     9781 gccagcctcg cgatcgacga cggcgaggcc gcgccgggcc cgctgcaggc caacgttgcc
     9841 aagaacctgg tcgcctggga gttcgcgcgc gacatgcgcg aggtcggacg catgctcgcc
     9901 gcgggcgagg tgcaagcggc ggtcgcacgg attgccgacc tcaggggctt gatcgccggc
     9961 ctgcgcctcg aggttcccgg ctggtcgggc gatcccgacc tcgccgccga cgagtcgttg
    10021 ctggacgcct atctcgcggt cctgcaatcg cccgccgccg gcgatgctgt gcaacgggac
    10081 tatctggcgg actccctgca ctacgccgcc ttccgcaagc ttcaagccgc ggcgcgatag
    10141 gtcatcccgc gtccggcccg gaggatccga ccatgaaaat ggtgcttgcc ttgctcgccc
    10201 tggtgctcgc gttgccggca ctcgcggcgc agcctgagga gctggtgccc gatgaaacgg
    10261 gcgaggtgcg cgtgccgctg gcggcgtaca cgg
//
LOCUS       DASWHU010000023         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1855471, whole genome shotgun sequence.
ACCESSION   DASWHU010000023 DASWHU010000000
VERSION     DASWHU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855471"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGB88_00805"
     CDS             <1..710
                     /locus_tag="VGB88_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9646004.1"
                     /translation="AAQAGQPPFKAPITDMRGILNLNVAGVLHPIFLNDTGITSLADI
                     AAREVPLRVDTGPRGTGGELAASRLLELYGAGYDNIRAWGGSITHSSYREALDRMKDG
                     HIDAFINDDIVGQPLFVDIALSRDVVFLPMTPEVVTEMVERFGYARASIPANTYKGQD
                     AEIPSTAQLFAFTCHKDLPEDLVYAMTRLTFEYRDELIATHKVFSDLSLEAGPTHFPI
                     PLHPGAERYYREVGVLP"
     gene            730..2649
                     /locus_tag="VGB88_00810"
     CDS             730..2649
                     /locus_tag="VGB88_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX9646005.1"
                     /translation="MVELSGQRRLVGLGGLVLRWGGVGIILYHLAAAQFGAPEVMKFR
                     PTHVAMYLGLIFLAFGWRRSDTRTSVPWYDWLLFALSWVPLAYIYANYEYVVERYAYI
                     TPLRALDWAMGLLALGLTVEACRRTVGLTLVALLGVFVVQALFGPYFPAPFDSSAVPP
                     MRLVDHLFLTTSGLYGSITGISATYVLMFVLLGAVLEQARGGDLFMNIATSVMGRAKG
                     GPAKAAVLASALFGSISGAAVANVYATGTFTIPLMIRTGFKPRFAAAVEAVASASGQL
                     VPPIMGSAAFLIADFTRTQYVDVAAAAALPAFLYLAAVYFMVHLETRRYGLSGMSAEL
                     VAKARREVWRDIHMLLVLAIVVAMLVERRTPFFAAYVGVVATLVVAQLRGHTRVGVKR
                     IMDGFEIGARRIAPIAAALFVAGLVVGVIELSGLGLRFTSILLSVTGGDLLATLLLVM
                     LSCIVLGMGLPTAAAYMIVAIFGAPALIKLGIEPLAAHFFVFYYAIISAITPPVAVAA
                     YAAATIAETPLQATGMSAMKLGAAVYLVPFVIAYSPALLLIGDAWQVVQAVATALVGI
                     VALAACVQGWLVRALSVPERVLAAAAALALLHGGWHTDLAGAVALAALVAWQLARGPR
                     AAPAQSGAGDEGASG"
     gene            complement(2646..>3673)
                     /locus_tag="VGB88_00815"
     CDS             complement(2646..>3673)
                     /locus_tag="VGB88_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9646006.1"
                     /translation="DRLALIRLMQTCSMLQALALFALTALDAISIGLLLVLVLINGIV
                     IGVNQPARLALVTALVPRADIAAAVAINSVTFNLARFIGPAVAGVLIVWVDISAAFAA
                     NALSFIAFLIVLARLRLAPEAPRAAAPRGLAGLVGDAVAGMRYVLGHPGVATLLGLML
                     ALALAVRPFIELLPGFAAEVFAGGAETLATLSATVGIGAVVAGAWLAQRGDPRGLTRI
                     TFAATALAAVAVLGFAASDRLWLALACVAVAGFALVAAGVCTQTLIQLSVDGAVRGRV
                     LSLYGLIFRGGPALGALAMGAASELAGLRLPLAAGALAMLALAVHAWRRRLGASAALE
                     ADAAPAD"
BASE COUNT          504 a         1355 c         1279 g          535 t
ORIGIN      
        1 tcgccgcgca agcgggccag ccgccgttca aggctccaat caccgacatg cgcggcatcc
       61 tcaacctcaa cgtcgccggc gtgctgcacc cgatcttcct caacgacacc ggcatcacct
      121 cgctggcgga catcgccgcc cgcgaggtcc cgctgcgcgt cgacaccggc ccgcgcggca
      181 ccggcggcga gctggcggcg agccggctgc tcgagctgta cggcgcgggc tacgacaaca
      241 tccgcgcctg gggtggctcg atcacccact cgtcctaccg cgaggcgctc gaccggatga
      301 aggacgggca catcgacgca ttcatcaacg acgacatcgt cggccagccg ctgttcgtcg
      361 acatcgcact gtcgcgcgac gtggtgttcc tgccgatgac gccggaggta gtcacggaga
      421 tggtcgagcg cttcggctat gcgcgggcga gcattcccgc caacacttat aagggacagg
      481 acgccgagat cccctcgacc gcccagcttt tcgccttcac ctgccacaag gacctgcccg
      541 aggacctggt ctacgccatg acccggctga ccttcgagta tcgcgacgag ctgatcgcaa
      601 cgcacaaggt gttcagcgac ctcagcctcg aggcgggacc gacgcacttc cccatcccgt
      661 tgcatccggg ggccgagcgc tactaccgcg aggtcggcgt gctgccatag gcgcgcccgg
      721 ggcccggcca tggtcgagct gagcgggcaa cgcaggctgg tcggcctcgg cgggctggtg
      781 ctgcgctggg gcggggtcgg catcatcctc taccacctgg ccgcggcgca gttcggcgcg
      841 cccgaggtga tgaagttccg ccccacccat gtcgccatgt atctggggct gatcttcctc
      901 gccttcggct ggcggcggag cgacacccga acctcggttc cgtggtacga ctggctgctg
      961 ttcgcgctgt cctgggtgcc gctcgcctac atctacgcga actacgagta cgtggtcgag
     1021 cgctacgcct acatcacgcc gctccgcgca ctcgactggg cgatgggcct gctcgcgctg
     1081 ggtctgacgg tcgaggcctg ccggcgcacg gtcgggctga ccctggtcgc gctgctcggc
     1141 gtgttcgtgg tccaggcgct gttcgggccc tacttcccgg caccgttcga ctcgagcgcg
     1201 gtgccgccga tgcgcctggt cgatcatctg ttcctgacca cctcgggcct ttacgggtcg
     1261 atcaccggca tctcggcgac ctacgtgctg atgttcgtgc tgctgggcgc ggtgctcgag
     1321 caggcgcgcg gcggcgactt gttcatgaac atcgccacct cggtgatggg ccgcgccaag
     1381 ggcgggccgg ccaaggcggc ggtgctggcg agcgccctgt tcggctcgat ctcgggcgcg
     1441 gcggtggcca acgtctacgc caccggcacc ttcaccatcc cgctgatgat caggaccggc
     1501 ttcaagcccc ggttcgccgc cgcggtcgag gcggtggcct cggccagcgg ccagctggta
     1561 ccgccgatca tgggctcggc ggcgttcctg atcgccgact tcacccgcac ccagtatgtc
     1621 gacgtcgccg cggcggcggc gctgcccgcg ttcctgtacc tggccgcagt ctacttcatg
     1681 gtccatctcg agacccgccg ctacggcctg tccggcatgt ctgccgagct ggtcgccaag
     1741 gcccggcgcg aggtctggcg cgacatccac atgctgctgg tgctggcgat cgtggtggcg
     1801 atgctggtcg agcggcgcac accgttcttc gccgcctatg tcggcgtggt cgccaccctg
     1861 gtcgtcgccc agctgcgcgg ccatacccgc gtcggcgtca agcggattat ggacggcttc
     1921 gagatcggcg cccggcgcat cgccccgatc gcggcggcgc tgttcgtcgc cggcctggtg
     1981 gtcggcgtga tcgagctcag cgggctgggg ctgcgcttca cctcgatcct gctcagcgtc
     2041 accggcggcg acctgctggc gaccctgctg ctggtgatgc tgagctgcat cgtgctcggc
     2101 atgggcctgc cgaccgccgc cgcctacatg atcgtcgcca tcttcggcgc gccggcgctg
     2161 atcaagctcg gcatcgagcc gctcgccgcg cacttcttcg tgttctacta cgcgatcatc
     2221 tcggcgatca cgccgccggt cgcggtcgcc gcctacgccg ccgcgaccat cgccgagacg
     2281 ccgctgcagg cgaccgggat gagcgccatg aagctcggcg ccgcggtcta cctggtgccg
     2341 ttcgtgatcg cctacagccc ggcgctgctg ctgatcggcg acgcctggca ggtggtccag
     2401 gcggtggcca ccgcgctggt cggcatcgtc gccctggcgg cgtgcgtgca gggctggctg
     2461 gtgcgcgcgc tgagcgtgcc cgagcgcgtg ctcgcggccg ccgccgcgct ggcgctgctg
     2521 cacggcggct ggcataccga ccttgccggc gccgtcgcgc tggccgcgct ggtcgcctgg
     2581 cagctcgcgc gcggcccgcg cgccgcgccc gcgcaaagcg gcgccggcga cgagggcgcc
     2641 tccggctagt cggccggcgc ggcgtcagcc tcgagcgcgg ccgaggcgcc gagccgccgc
     2701 cgccaggcat gcaccgccag cgccagcatc gccagcgcgc ccgcggccag cggcagcctg
     2761 agcccggcga gctcggaggc agcgcccatg gcgagcgcgc cgagcgccgg cccgccgcgg
     2821 aagatcagcc cgtacaggct cagcacccgc ccgcgcacgg cgccgtcgac cgagagctgg
     2881 atcagggtct gggtgcacac ccccgccgcg accagggcga agcccgccac cgcgacgcag
     2941 gcgagcgcga gccacagccg gtcgctggcg gcgaagccga gcacggcgac ggcggcgagc
     3001 gcggtggccg cgaacgtgat ccgcgtgagg ccgcgagggt cgccccgctg ggcgagccac
     3061 gcgccggcga cgaccgcgcc gatgccgacc gtggcgctga gggtggcaag ggtctcggcg
     3121 ccgcccgcga acacctccgc cgcgaagccc ggcagcagct cgatgaacgg ccgcaccgcg
     3181 agcgcaagcg cgagcatcag cccgagcagg gtggcgacgc ccggatggcc gagcacgtag
     3241 cgcatgccgg ccacggcatc gccgaccagg ccggcgaggc cgcgcggcgc ggccgccctc
     3301 ggcgcctccg gtgccagccg caggcgcgca agcacgatca ggaaggcgat gaagctgagc
     3361 gcattggcgg cgaacgcggc ggaaatgtcg acccacacga tcagcacccc ggccactgcc
     3421 gggccgatga accgtgccag gttgaacgtc accgagttga ttgccaccgc ggcggcgatg
     3481 tcggcgcgcg gcaccagcgc cgtcaccagc gcgagccgcg ccggctggtt gaccccgatc
     3541 acgatgccgt tgatcagcac cagcaccaac agcaggccga tcgagatcgc atccagtgcg
     3601 gtgagcgcga acaacgccag cgcctgaagc atcgagcagg tttgcatcag ccggatcagg
     3661 gccagccggt cga
//
LOCUS       DASWHU010000024         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2596194, whole genome shotgun sequence.
ACCESSION   DASWHU010000024 DASWHU010000000
VERSION     DASWHU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596194"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VGB88_00820"
     CDS             <1..1098
                     /locus_tag="VGB88_00820"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX9646007.1"
                     /translation="QYLTLAGEPLLRRSVRAFAGHPAVEAVRVVIHPADRAIYDQAVA
                     DLAVLDPVAGGEARQDSVRNGLESFAAEPPHRVLIHDAARPLVGAATITRTLEALAEF
                     AGAIAAVPVRDTLKRASGDDRLIAETVTRAGLWRAQTPQAFRFADILDAHRAHAGLDL
                     TDDAQVAERHGLRVVLVEDSEDNLKVTTAADLVRAERLLAGVRTDQVRIGTGFDVHRF
                     AADGDHVMLCGVKVPHARGLTGHSDADVGLHALVDAVLGALAAGDIGTHFPPGEERWK
                     GADSAAFVEHGRRLAVAAGARIENVDITVICERPRIGPYRQAMRARVALLLGIDAGRV
                     SIKATTTEGLGFAGRGEGIAAQAVASLRVGP"
     gene            1095..1574
                     /locus_tag="VGB88_00825"
     CDS             1095..1574
                     /locus_tag="VGB88_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX9646008.1"
                     /translation="MTARDRRRAAWRPTTLIATWFGTGFLPVAPGSWGSLAALPFAWG
                     LEWLGGQALLLAAALAAFALGWWAAGAYAARTGDDDPGAVVIDEVAGQWLALAFVPAS
                     VWSYALGFVLFRIADIAKPWPASLAERRLAGGLGVMADDMVAGAYAGLGLLVVERLI"
     gene            1571..2068
                     /locus_tag="VGB88_00830"
     CDS             1571..2068
                     /locus_tag="VGB88_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX9646009.1"
                     /translation="MSPFPAHLVEAAASLLDDYRARGVKLATAESCTGGLLAGCLTEV
                     AGSSDVVERGFVSYSNEAKIELLGVPPALIAAHGAVSEPVARAMAEGALARSRADVAL
                     SVTGVAGPGGGSAEKPVGLVHFAVAGRGRTTTAHVETFAGDRATIRLAAVARALALLS
                     AALES"
     gene            2343..3281
                     /locus_tag="VGB88_00835"
     CDS             2343..3281
                     /locus_tag="VGB88_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9646010.1"
                     /translation="MSRRGVVKGAGALAAAAAMGSAMTGASSTARAAAPGKVGHVTTS
                     DGVSLHYLEAGSGKPLLMIPGWSQTAEQFKHQVAGLSDRYHVIALDMRGHGESDKPDH
                     GYKIDRLAKDVRDVIYALNLTDVNILGHSMGCSVIWNYWDLWGPDRLSKLLLIDQMPM
                     ITSNPAWSEQEIAEAGCIFTPESLYATVNDLAGPNGVEVTRGFIGNMVTKTASEEVKN
                     WIIERNLRMPRQHAATLLYDHSTTDWRDTIPRLDRPSLVVGGRVSVVPWKSQVWINQN
                     VAGSRLEIFEENEGGNHFMFIEAYQKFNDILADFIA"
     gene            complement(3380..3862)
                     /locus_tag="VGB88_00840"
     CDS             complement(3380..3862)
                     /locus_tag="VGB88_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX9646011.1"
                     /translation="MPVHHERRRLPYTAAQVFDIVAAVDRYPEFLPWCLSARITRREA
                     DSFEADLVIGFKMVRERFASRVLLERPHRIVARETSGPFRRLNNLWHFTDLGEGGCEV
                     DFHIDFEFRSRLLNKLIGLLFHEAVRRLVAAFDGRARALYGTGQGTLARAASDQLTSS
                     "
     gene            complement(3865..4791)
                     /gene="lipA"
                     /locus_tag="VGB88_00845"
     CDS             complement(3865..4791)
                     /gene="lipA"
                     /locus_tag="VGB88_00845"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9646012.1"
                     /translation="MTGTAQLHATAPGARPRKPEWIRVKAPTSPGYHATRKLMRALSL
                     NTVCEEAACPNIGQCWARGHATVMILGSVCTRKCAFCNVASGRPDRLDPHEPDHLAAA
                     VAELGLGHVVITSVDRDDLDDGGAGQFVRCIERIRATSPGTTIEVLTPDFLRKPGAVE
                     AVVAARPDVYNHNLETVPRLYKTVRPGARYEQSLWLLGRVRELDPAIFTKSGLMVGLG
                     ETEDEVLAVMDDMRARDIDFLTVGQYLQPTPGHHPVIRFVTPEEFTGLARAATAKGFL
                     MVSASPLTRSSYLADDDFQKLRRARRAAAVRG"
     gene            complement(4795..>4939)
                     /locus_tag="VGB88_00850"
     CDS             complement(4795..>4939)
                     /locus_tag="VGB88_00850"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491181.1"
                     /note="E3 component of alpha keto acid dehydrogenase
                     complexes LpdC; forms a homodimer; binds one molecule of
                     FAD monomer; catalyzes NAD+-dependent oxidation of
                     dihydrolipoyl cofactors that are covalently linked to the
                     E2 component; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9646013.1"
                     /translation="ELIQGFTVAKTMEGTELDLIHSIFPHPTLSETMHEAVLDAYGRV
                     IHI"
BASE COUNT          723 a         1728 c         1767 g          721 t
ORIGIN      
        1 caatatctga ccctcgcggg cgagccgctt ctgcgccgct cggtgcgcgc cttcgccggc
       61 cacccggcgg tcgaagcggt gcgggtggtg atccacccgg ccgatcgcgc aatctatgac
      121 caggcggtcg ccgacctcgc cgtgctcgac ccggtcgcgg gcggcgaggc gcggcaggat
      181 tcggtgcgca acggcctgga atcgttcgcc gcggagccgc cacaccgggt gctgatccac
      241 gacgcggcac ggcctctggt cggcgcggcg accatcacgc gcaccttgga ggcgctggcc
      301 gagtttgccg gcgcgatcgc cgcggtgccg gtgcgcgata cgctcaagcg tgcctcgggc
      361 gacgaccgcc tgatcgccga gaccgtaaca cgtgccgggc tgtggcgcgc gcagacgccg
      421 caggcgttcc gcttcgccga catcctcgac gcccaccgcg cccatgccgg cctcgacctc
      481 accgacgacg cccaggtcgc cgagcgccac ggcctgcgtg tggtcctggt cgaggactcg
      541 gaggacaacc tcaaggtgac cacggccgcg gacttggtcc gcgccgagcg cctgctcgcc
      601 ggcgtgcgca ccgaccaggt ccggatcggc accggcttcg acgtccaccg cttcgccgcg
      661 gacggcgatc acgtcatgct gtgcggggtc aaggtcccgc acgcgcgcgg cctcaccggc
      721 cactcggatg ccgatgtcgg cctgcacgct ctggtcgacg cggtgctcgg cgcgctcgcc
      781 gccggcgaca tcggcaccca ctttccgccc ggcgaggagc gctggaaggg cgccgactcc
      841 gcagccttcg tcgagcacgg gcgcaggctc gcggtcgctg ccggggcgcg catcgagaac
      901 gtcgacatca ccgtgatctg cgagcgcccg cgcatcggtc cctatcgcca ggcgatgcgc
      961 gcgcgcgtcg ccctgctgct cggcatcgac gcgggccggg tcagcatcaa ggcgaccacc
     1021 accgaaggcc tcggcttcgc cggccgcggc gaaggcatcg ccgcccaggc ggtggcgagc
     1081 ctgagggtcg gcccgtgacc gcgcgcgacc gtcgtcgcgc cgcctggcga ccgacgaccc
     1141 tgatcgccac ctggttcggc accggcttcc tgccggtggc gccggggagc tggggctcgt
     1201 tggcggcgct gccgttcgcc tgggggctcg aatggctggg cggccaggcg ctgctgctgg
     1261 ccgcggcatt ggccgcgttc gccctcggct ggtgggcggc cggcgcctat gcggcgcgca
     1321 cgggcgacga cgacccgggc gcggtggtga tcgacgaggt tgccggccag tggctcgcgc
     1381 ttgcgttcgt gcccgcctca gtttggagct acgcgctcgg cttcgtgctg ttccgcatcg
     1441 ccgacatcgc caagccgtgg ccggcctcgc tcgccgagcg ccggctcgcg ggcggcctcg
     1501 gggtgatggc cgacgacatg gtcgccggcg cctatgccgg cctcgggctg ctggtcgtgg
     1561 agcggctgat atgagcccct tccccgccca cctggtcgag gcggcggcga gtctgctcga
     1621 cgactatcgt gcgcgcggcg tcaagcttgc cacggcggag tcgtgcaccg gcggcctcct
     1681 ggccggatgt ctcaccgagg tcgccggctc ctccgacgtg gtcgagcgcg gctttgtcag
     1741 ctactcgaac gaggccaaga tcgagctcct cggcgtgccg ccggcgctga tcgccgccca
     1801 cggcgcggtc agcgagccgg tcgcgcgcgc gatggccgaa ggcgcgctcg cccgttcccg
     1861 cgccgatgtc gcgctgtcgg tgacaggcgt cgcgggcccc ggcggcggca gcgcggagaa
     1921 gccggtcggc ctggtccact tcgccgtcgc cggccgcggc cgcacgacca cagcgcatgt
     1981 cgagactttc gccggcgacc gcgccaccat ccgcctcgcc gccgtcgccc gcgcgctcgc
     2041 gctgctgtcc gccgcgctcg agagctgagc ggtcacgcgc gcacgccccg atcacgcgcc
     2101 cggcagtgcg gtctcgggca cctcgaatgg ctcctcgtta aaccaagccg gcccgtacgc
     2161 ttgctgggcg accccccgag tggcactatc gccgcgacgg acgaccatct gggaacgtcc
     2221 gagaaacata gggagccgtt ggccgaagag ttcgaccgtt cccgaactgg gaggaaatct
     2281 aggtgacgac gagaggggaa gatcagttca cgacgcgtga ccgtgccaat gatcggctga
     2341 ggttgagccg ccggggcgtg gtcaaggggg ccggcgctct cgccgccgcc gcggcgatgg
     2401 ggtcggcgat gaccggtgcg agctcaaccg cgcgcgcggc cgctccgggc aaggtcggtc
     2461 acgtaacgac cagcgacggc gtcagcctcc attatctgga ggccggttcc ggcaagccgt
     2521 tgctgatgat acccggctgg tcgcagaccg ccgagcagtt caagcaccag gtcgcggggc
     2581 tcagcgaccg ctaccatgtc atcgccctcg acatgcgcgg ccacggcgaa tccgacaagc
     2641 cggaccacgg ctacaagatc gaccgtctcg ccaaggacgt tcgcgacgtc atctatgcgc
     2701 tcaacctgac ggacgtcaac atcctcggcc attcgatggg ctgctcggtg atctggaact
     2761 actgggacct gtggggaccg gatcgtctgt ccaagctgct cctgatcgac cagatgccga
     2821 tgatcaccag caacccggcg tggtccgagc aggagatcgc cgaagccggg tgcatcttca
     2881 cgccggagtc gctctacgcc acggtcaacg acctggccgg ccccaacggc gtcgaggtga
     2941 ctcgcggctt catcggcaac atggtgacca agacggcctc ggaagaggtc aagaactgga
     3001 tcatcgagcg caacctgcgc atgccgcgcc agcacgcggc gacgctgctc tacgaccaca
     3061 gcacgaccga ttggcgcgac acgatcccgc gcctcgacag gccctcgctg gtggtcggcg
     3121 gccgggtcag cgtggtgccg tggaagtcgc aggtgtggat caaccagaat gtcgccggct
     3181 cgcgcctcga gatcttcgag gagaacgagg gcggcaatca cttcatgttc atcgaggcct
     3241 accagaagtt caacgacatc ctcgccgact tcatagcctg agccacgcgg acgaacgggg
     3301 cgcgcgatcg cccggcggcg acatcgatcg ccgggtggcc gcgcgccctg gatcgaagcc
     3361 ggggcgcgcc ggagctcctc tacgacgacg tgagctgatc ggaggccgcg cgcgcgagcg
     3421 tgccctggcc cgtgccgtag agcgcgcgcg cgcggccgtc gaacgccgcg accaagcgcc
     3481 ggacggcctc gtggaacagg aggccgatca gcttgttgag caagcgcgag cggaactcga
     3541 agtcgatgtg gaagtcgacc tcgcagccgc cctcgcccaa gtcggtgaag tgccacaggt
     3601 tgttgaggcg gcggaacggg cccgaggtct cgcgcgcgac gatgcggtgc gggcgctcga
     3661 gcaggacccg cgaggcgaag cgctcgcgga ccatcttgaa gccgatcacg agatccgcct
     3721 cgaacgagtc cgcctcgcgc ctggtgatgc gcgcagacag gcaccagggc aggaactccg
     3781 ggtagcggtc gaccgcggcg acgatgtcga acacctgcgc cgcggtatag ggcagcctgc
     3841 gccgttcgtg gtggacgggc atgatcagcc gcggacggcg gccgcccggc gcgctctgcg
     3901 cagcttctgg aagtcgtcgt cggcgaggta cgacgagcgc gtcaagggcg aggccgagac
     3961 catcaggaag cccttggcgg tggccgcgcg cgcaagcccc gtgaactcct ccggggtgac
     4021 gaagcggatc accgggtggt ggccgggcgt gggctgcagg tactgaccga cggtgaggaa
     4081 gtcgatgtcg cgcgcgcgca tgtcgtccat cacggcgagc acctcgtcct cggtctcgcc
     4141 gaggccgacc atcaggccgg acttggtgaa aattgcgggg tcgagctcgc gcacgcggcc
     4201 cagcagccac agcgactgct catagcgcgc gcccgggcgc accgtcttgt acaggcgcgg
     4261 cacggtctcg aggttatggt tgtagacgtc gggacgcgcc gccaccaccg cttccaccgc
     4321 gcccggcttg cgcaggaaat cgggcgtcag cacctcgatg gtggtgcctg gcgaggtcgc
     4381 gcggatgcgc tcgatgcagc gcacgaactg gccggcgccg ccgtcgtcga ggtcgtcgcg
     4441 gtcgaccgag gtgatcacca cgtggcccag gccgagctcc gccaccgccg cggcgagatg
     4501 gtcgggctcg tgcgggtcga gccggtcggg ccggccgctg gcgacgttgc agaacgcgca
     4561 tttgcgcgtg cacacgctgc ccaggatcat caccgtcgca tggccgcgcg cccagcactg
     4621 gccgatgttg gggcatgccg cctcctcgca cacggtattg agcgacagcg cgcgcatcag
     4681 cttgcgggtg gcgtgatagc ccggcgaggt cggcgccttg acccggatcc attccggctt
     4741 gcgcggacgc gcgccgggcg cggtggcgtg gagctgcgcg gtaccggtca tggattagat
     4801 gtggatgacc cggccatagg cgtcaagcac cgcctcgtgc atggtctcgg acagcgtcgg
     4861 gtgcgggaag atcgaatgga tcaggtcgag ctcggtgcct tccatggtct tggcgaccgt
     4921 aaatccttgg atcagctcg
//
LOCUS       DASWHU010000025         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1116351, whole genome shotgun sequence.
ACCESSION   DASWHU010000025 DASWHU010000000
VERSION     DASWHU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116351"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /gene="dnaE"
                     /locus_tag="VGB88_00855"
     CDS             complement(<1..1277)
                     /gene="dnaE"
                     /locus_tag="VGB88_00855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9646014.1"
                     /translation="MPHADFVHLRVHTAYSLSEGAIRIGELVELCRSQAMPAVAITDS
                     GNLFGALEFSLACADAGIQPIIGCQLALGGGREAAPRPERGAADHLVVLVQDQAGYGN
                     LLKLVSKAYLESDGGAPRLVLDDLDGLSDGLLALTGGAQGGVGRLLAEGQVDAAESLI
                     ERLKCLFPDRLYVELQRHGSDLERRIEPHLIALAHSHDLPLVATNEAFFTGPEMHEAH
                     DALLCIANSTYLSQAERPRVTPEHSFKSAPAMRALFADLPEAVDNTLAVARRCAFMVA
                     ARPPLLPASPKVAPGQSEAEALREIAEAGLEDRLARHVLRPGTDETARAAAAKPYRER
                     LAYELGVISEMGFAGYFLIVADFIQWAKAQGIPVGPGRGSGAGSVAAWALKITDLDPL
                     RFGLLFERFLNPARISMPDFDIDFCQDRRDEVI"
     gene            1394..>1785
                     /locus_tag="VGB88_00860"
     CDS             1394..>1785
                     /locus_tag="VGB88_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEX9646015.1"
                     /translation="MAAESSTATVTSEDALRALYDPPLERAVRKQLSRLDDYCKRFIA
                     LSPFVCLATADADGRIDVSPRGDAPGFVEVVDDRTLLIPDRRGNNRLDSLANIIANPQ
                     VGLLFMVPGIDETVRVNGRAAIVRDPDML"
BASE COUNT          262 a          632 c          639 g          252 t
ORIGIN      
        1 tggatcacct cgtcgcgccg atcctggcag aagtcgatgt cgaagtcggg catcgagatg
       61 cgcgccgggt tgaggaagcg ctcgaacagg agtccgaagc gcagcgggtc gaggtcggtg
      121 atcttgagcg cccaggcggc gaccgagccc gcgcccgagc cgcgtccggg gccgaccggg
      181 attccttgcg ccttggccca ttggatgaag tcggcgacga tcaggaagta gccggcgaac
      241 cccatctcgc tgatcacgcc gagctcgtag gcgagccgct cgcgataggg cttggccgcg
      301 gcggcgcgtg cggtctcgtc cgtgccgggg cgcagcacgt gcctggcgag cctgtcctcc
      361 agcccggcct cggcgatttc gcgcagcgcc tcggcctcgc tctgcccggg cgccaccttg
      421 ggcgaggccg gcagcagcgg cgggcgcgcc gcgaccatga atgcgcagcg cctggccacc
      481 gcgagcgtgt tgtcgaccgc ctcgggcaaa tccgcgaaca gcgcccgcat cgccggcgcg
      541 gacttgaagg agtgctcggg cgtcacccgc gggcgctcgg cctgcgacag gtaggtgctg
      601 ttggcaatgc acagcagcgc atcgtgcgcc tcgtgcatct cggggccggt gaagaacgcc
      661 tcgttggtcg ccaccagcgg caggtcgtgg ctgtgcgcaa gcgcgatcag atgcggctcg
      721 atgcgccgct cgaggtcgct gccgtggcgc tgcagctcga catagagccg gtccggaaac
      781 aggcacttga gccgctcgat caggctttcg gcggcgtcga cctggccctc ggcaagcagc
      841 cgcccgacgc cgccctgcgc gccgccggtg agcgcgagca ggccgtcgct caagccgtcg
      901 aggtcgtcga ggacgagcct gggcgcgccg ccgtcgcttt cgagataggc cttgctgacc
      961 agcttgagca ggttgccgta gccggcctgg tcctggacca gcaccaccag gtgatcggcg
     1021 gcgccgcgct cgggcctcgg ggcggcctcg cgcccgccgc ccagcgccag ctggcagccg
     1081 atgatcggct ggatgccggc gtcggcgcag gcgagcgaga actcgagcgc gccgaacagg
     1141 ttgccggaat ccgtgatcgc caccgccggc atcgcctggc tgcggcacag ctcgaccagc
     1201 tcgccgatgc ggatcgcgcc ttccgacagc gaataggcgg tgtggacgcg aagatggacg
     1261 aagtctgcgt ggggcatggc gctcaggtct ggtgacgcgc aaacatggca cggggcgggc
     1321 gccgtgtcag ccgatggacg cggcccccca cctcgcctaa agtcgcggcc ggacgcccgc
     1381 gcggagaggc ctcatggccg ccgaatcatc gaccgcgacc gtgaccagcg aggatgcgct
     1441 gcgcgccctc tacgacccgc cgctcgagcg cgcggtgcgg aagcagctca gccgtctcga
     1501 cgattactgc aagcgcttca tcgcgctgtc gccgttcgtg tgcctggcca ccgccgacgc
     1561 cgacggccgg atcgacgtga gcccgcgcgg cgacgccccc ggattcgtcg aggtggtcga
     1621 cgaccgcacc ctcttgatcc ccgaccggcg cggcaacaac cgcctcgatt cgctcgccaa
     1681 catcatcgcc aacccgcagg tcggcctgct gttcatggtg ccggggatcg acgagacggt
     1741 gcgggtcaac ggccgcgccg cgatcgtccg cgacccggac atgct
//
LOCUS       DASWHU010000026         6656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_747511, whole genome shotgun sequence.
ACCESSION   DASWHU010000026 DASWHU010000000
VERSION     DASWHU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747511"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="VGB88_00865"
     CDS             complement(<1..86)
                     /locus_tag="VGB88_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX9646016.1"
                     /translation="MAVTIYHNPRCSTSRRTLELLRARGIEP"
     gene            complement(77..520)
                     /locus_tag="VGB88_00870"
     CDS             complement(77..520)
                     /locus_tag="VGB88_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646017.1"
                     /translation="MAIDDQMQPQGQHDLLDSFIRLIAAAIDATARYTGGHCAGARDH
                     GNAGGRRLCLDHAAEEREQVRACAGCRAAALAAYRARRWTDREHILRLYGERACGWPI
                     ARFYDMLSGRIAGVRARSPGADGDGVTVAATKWPARTTREDEPWP"
     gene            800..1633
                     /locus_tag="VGB88_00875"
     CDS             800..1633
                     /locus_tag="VGB88_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9646018.1"
                     /translation="MTTEQARTGAGRGGFGPVQRSFGAVNWIGLWTLYVKEVRRFLKV
                     PGQTLLAPCATTLLYLAIFALALGGAVREIHGIPFLAFLGPGLIAMAIVQNAFANTVT
                     SVMISKVQGNIVDMLVPPLSPGELTLAFALGGLTRGVAVGVAVAVAIFAFVPTAVHDW
                     AAVVCYGLGASLMLSLVGLATAVWADKFDQTAAITNFVVTPLAFLSGTFYSIDRLPEP
                     WHAISLFNPFFYMIDGMRYGFTGHADGSIAVGAAVLVAADLALYGLCYWMFATGYKLK
                     E"
     gene            1755..2924
                     /locus_tag="VGB88_00880"
     CDS             1755..2924
                     /locus_tag="VGB88_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9646019.1"
                     /translation="MSAVMPTYARIDIAFERGEGPYLYATDGRRYLDFATGIAVNTLG
                     HCHPHLVAALGEQAAKLWHCSNLYRIPEQERLAERLCAHSFADKVFFCNSGAEAMEGV
                     IKIARRYHHQSGHPERWRLIAFEGAFHGRTLATLAAGGNQKALAGFDPPVEGFDHVAF
                     GNANEVRAAITGHTGAILVEPIQGEGGIRPADTRFLKDLRAIADEYGLLLLFDEVQTG
                     VGRTGKLFAYEWHGVAPDVMGLAKGLGGGFPVGAVLATDAAAAAMTPGTHGSTFGGNP
                     LAVAAANAVLDVLLEDGFLKRVGALGKALAKRVGDVAARHGDVIELVRGQGLMLGMRV
                     RATPADLVAAMRERGVLVVAAADNVVRMLPPLNIEQSHVEEAAGALEAACAGLGK"
     gene            2921..3832
                     /gene="argF"
                     /locus_tag="VGB88_00885"
     CDS             2921..3832
                     /gene="argF"
                     /locus_tag="VGB88_00885"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9646020.1"
                     /translation="MNAPRHFLDLDAFDRDTLRAILDRAVAAKRAARGARGPGALAGR
                     VLAMIFEQPSTRTRTSFEVAMIQLGGAAITLNAGESQLGRGETIADTARVLSRYVDAI
                     VLRALRHASLIELARHAAVPVINGLTDRSHPCQLMADVMTVEERRGAIEGQVVAWSGD
                     ANNVAASWVHAAVRLGFELRLACPAALAPDPSLIAWAKGEGGRVSVTESAAAAVAGAD
                     CVVTDTWVSMYDRPGDRAALLAPYRVDRALMAAAKPDALFLHCLPAHRGQEVTGEVID
                     GPQSAVWDEAENRLHAQKAVLLWCFGQ"
     gene            3829..4782
                     /locus_tag="VGB88_00890"
     CDS             3829..4782
                     /locus_tag="VGB88_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX9646021.1"
                     /translation="MSADANQPGHPLAPADDLIQPFQVGALDANGRLVRLGAAVDRIL
                     SQHDYPEPVSILLGEAMAMTAALAASLKFEGTFSTQIQGDGPVRLMIADVRTDGAMRA
                     YAGYDAERVAAEAQAAAPGAVGDAGLFGAGHMAFTIDQGGDRDRYQGLVELEGATLAD
                     CAQAYLTRSEQLRADISTAAVRWPERTGGASWRAGCLLVQQVPAGDGRGGDDLARAAN
                     WTETRALVGRLRRVDLADPLAVPPALIAPLFPGRDIRLFDPRPLRFECRCTAARAARV
                     LQVLGPEERAELMIDGRIEVTCDYCNTRYLFDPDGKLIADA"
     gene            4955..5563
                     /locus_tag="VGB88_00895"
     CDS             4955..5563
                     /locus_tag="VGB88_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX9646022.1"
                     /translation="MPRLAAVPWRSALPVLLILLILSGCTGRTARQASPVLPETLPAV
                     GSELRPQMVAVRRPVQCVAYARALSGLPIRGDAWTWWPAADGRYLRNRTPRPGALLVL
                     ERTRRLRHGHLAMVTHVLGPRQILVDHANWLNQGRIHRHTPVVDVSPGNDWSLVRVWY
                     IPGNRLGSNSYPAYGFVHPEALTAAAPSPARRNASFPPARKS"
     gene            5698..6285
                     /locus_tag="VGB88_00900"
     CDS             5698..6285
                     /locus_tag="VGB88_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646023.1"
                     /translation="MTPLRLPIAVVLVALAAACAPPPAEPRFPEVTFRDQAPFRFDAA
                     TVEVVEAFQPPLKVPHVEHEVPVPPAAMLKRWVADRVVAAGAEGRVRATIVDASIVET
                     PLATNQTIAAGLTTEQAVRFDARVELELEYLDARGVPKGAVRGEARRSRTVPEDATLR
                     ERDEGLFQMTEDLVNDLDRVLSANIPRYLGTHLVQ"
     gene            6288..>6656
                     /locus_tag="VGB88_00905"
     CDS             6288..>6656
                     /locus_tag="VGB88_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646024.1"
                     /translation="MPRKAGLCVLAAVTLAACAPLPPELQFPEISFADRAPFRFDAAA
                     VEVVEAYRPPLESPHVEHEAPVAPGTALRHWVDQRVAAAGSRGRVRVTIEDASIVETP
                     LATNRDLEASLTTERAARFEG"
BASE COUNT          874 a         2384 c         2405 g          993 t
ORIGIN      
        1 ctgggctcga tgccgcgcgc gcgcagcagc tccagggtcc ggcgcgaggt gctgcagcgc
       61 gggttgtggt agatggtcac ggccatggct cgtcctctcg tgtcgtgcgc gccggccact
      121 tggtcgcggc gacggtgacc ccgtccccgt ccgctcccgg cgagcgggcg cggacgccgg
      181 caatgcgacc cgacagcatg tcgtagaaac gcgcgatcgg ccagccgcaa gcacgctcgc
      241 catacaacct taggatgtgc tcgcggtcgg tccagcgccg ggcgcgatag gcggcgaggg
      301 cggcggcacg gcatccggcg caggcacgga cctgttcgcg ctcctcggcg gcgtggtcga
      361 ggcacaggcg gcgtccgcca gcatttccgt gatcacgggc acccgcgcaa tggccgccgg
      421 tgtagcgggc cgtggcatcg attgccgcag caatcaatcg gatgaacgag tcgagcaggt
      481 cgtgctgccc ttgcggctgc atctggtcgt cgatcgccac ccccgccggt ccgactcatt
      541 gcggccgatc accggcacgg cggcgacgcg ctcgctcacc tgccacctcc tcgccggggc
      601 gcgcggcccg gcctcgcggt ccgcgcgcgc cgcccgcagc cggacttcgc gtcctccgca
      661 acgacgcagc cgagggccct tgtcagccag atctcgcgtc ggcgcgggcc ggcatggaca
      721 gggaggggaa tatattgcag gttcggggcg agcaaagcgc gggccgccgg cccgcgccgg
      781 cacgcgaagg gacccctcga tgaccacgga acaggcgcgc accggcgcgg gcaggggcgg
      841 cttcggcccg gtccagcgat cgttcggcgc ggtcaactgg atcggcctgt ggacgctcta
      901 cgtcaaggag gtgcggcgct tcctcaaggt gccggggcag acgctgctgg cgccgtgcgc
      961 gaccacgctg ctgtacctcg ccatcttcgc gctcgccttg ggcggtgccg tgcgcgagat
     1021 ccacggcatc ccgttcctcg ccttcctcgg tcccgggctg atcgcgatgg cgatcgtcca
     1081 gaacgcgttc gccaacaccg tcacctcggt gatgatctcg aaggtgcagg gcaacatcgt
     1141 cgacatgctg gtgccgccct tgagcccggg cgagctcacg ctcgccttcg ctctcggcgg
     1201 gctgacgcgc ggggtcgcgg tcggggtggc ggtcgcggtc gcgatcttcg ccttcgtccc
     1261 gaccgcggtc cacgactggg cggcggtggt ctgctacggc ctcggcgcgt cgctgatgct
     1321 gtcgctggtc ggcctcgcca ccgcggtctg ggccgacaag ttcgaccaga ccgcggcaat
     1381 caccaacttc gtggtcacgc cgctcgcgtt cctgtccggc accttctact cgatcgaccg
     1441 gctgcccgag ccgtggcacg cgatcagcct gttcaacccg ttcttctata tgatcgacgg
     1501 catgcgctac ggcttcaccg gccacgccga cggctcgatc gcggtcggcg cggcggtgct
     1561 ggtggcggcc gacctggcgc tctacgggct gtgctactgg atgttcgcga ccggctacaa
     1621 gctcaaggaa tagcggcccg ccggggcgcc cgcgcccggc cattgacaag gtgggccgga
     1681 aaccgctact gccgcgcttt cccgacggtg cggacgggcc accgggcgcc gtcgcgagcg
     1741 aaccggagag tgccatgtcc gcggtcatgc cgacttacgc acgcatcgac atcgcgttcg
     1801 agcggggcga gggcccctat ttgtacgcga ccgacgggcg gcgctatctc gacttcgcca
     1861 ccggcatcgc ggtcaacacg ctcggccatt gccacccgca tctggtggcg gcgctgggcg
     1921 agcaggcggc gaagctctgg cattgctcca acctgtaccg catcccggag caggagcgcc
     1981 tggccgagcg gctgtgcgcg cacagctttg ccgacaaggt gttcttctgc aactcgggcg
     2041 ccgaggcgat ggagggcgtg atcaagatcg cgcgccgcta ccatcaccaa tccggacatc
     2101 ccgagcgctg gcggttgatc gcgttcgagg gcgccttcca cggccgcacg ctcgcaaccc
     2161 tcgccgccgg cggcaaccag aaggccttgg cgggcttcga tccgccggtc gagggcttcg
     2221 accacgtcgc gttcggcaac gccaacgagg tgcgcgccgc gatcaccggg cacaccggag
     2281 cgatcctggt cgagccgatc cagggcgaag gcggaatccg cccggcggac acccggttcc
     2341 tcaaggacct gcgtgcgatc gccgacgagt acggcctgct gctgctgttc gacgaggtgc
     2401 agaccggggt ggggcggaca ggcaaactgt tcgcctacga atggcatggc gtggcgcccg
     2461 acgtgatggg cctggccaag ggcctgggcg gcggcttccc ggtcggcgcg gtgctggcga
     2521 ccgacgccgc ggcggcggcg atgacgcccg gcacccacgg ctcgaccttc ggcggcaacc
     2581 cgctggcggt ggcggcggcg aacgccgtgc tcgacgtgct gctggaggac gggtttctga
     2641 agcgcgtcgg cgcgctcggc aaggcgctcg ccaagcgcgt cggcgatgtc gccgcccgcc
     2701 acggcgacgt catcgagctg gtgcgcggcc aggggctgat gctcggcatg cgcgtgcgcg
     2761 ccaccccggc cgacctggtc gcggcgatgc gcgagcgggg cgtgctggtg gtcgccgcgg
     2821 ccgacaacgt ggtgcgcatg ctgccgccgc tcaacatcga gcagagtcat gtcgaggaag
     2881 cggccggcgc cctcgaggcg gcgtgcgccg ggctcgggaa atgaatgcgc cacgtcattt
     2941 cctcgacctc gacgccttcg accgcgacac gctgcgcgcg atcctcgacc gggcggtcgc
     3001 ggccaagcgc gcggcgcgcg gcgcgcgcgg cccgggcgcg ctcgcgggcc gggtgctggc
     3061 gatgatcttc gagcagccct cgacgcggac ccgcacctcg ttcgaggtgg cgatgattca
     3121 gctcggcggc gccgcgatca cgctcaatgc cggcgagagc cagctcggcc gcggcgagac
     3181 catcgccgat accgcgcgcg tgctgtcgcg atatgtcgat gcgatcgtgc tgcgcgcgct
     3241 gcgccacgcg agcctgatcg agcttgcccg ccacgccgcg gtgccggtga tcaacgggct
     3301 gaccgaccgc agccacccgt gccagctgat ggccgacgtc atgacggtgg aggagcgccg
     3361 cggcgcgatc gagggccagg tggtggcgtg gagcggcgat gccaacaacg tcgcggcgtc
     3421 gtgggtccac gccgcggtca ggctcggctt cgagctcagg ctcgcctgcc cggccgcgct
     3481 cgcccccgat ccctcgctca tcgcctgggc gaagggcgag ggcgggcggg tgagcgtgac
     3541 cgagtccgcc gccgcggcgg tcgccggcgc cgattgcgtg gtcaccgaca cctgggtctc
     3601 gatgtacgac cggccgggcg accgggcggc gctgctcgcc ccttaccggg tcgaccgcgc
     3661 gctgatggcc gcggccaagc ccgacgccct gttcctgcac tgcctgccgg cgcatcgcgg
     3721 ccaggaggtg acgggcgagg tgatcgacgg cccgcaatcg gcggtctggg acgaggccga
     3781 gaaccgcctg cacgcgcaga aggcggtgct gctgtggtgc ttcggccaat gagcgcggac
     3841 gccaaccaac ccgggcatcc gctggcgccc gccgacgacc tgatccagcc gttccaggtc
     3901 ggcgcgctcg acgccaacgg ccggctggtg cgcctcggcg ccgcggtcga ccgcatcttg
     3961 agccagcacg actatccgga gccggtgtcg atcctgctcg gcgaggcgat ggcgatgacc
     4021 gcggcgctcg ccgcctcgct caagttcgag ggcaccttca gcacccagat ccagggcgac
     4081 ggcccggtca ggctgatgat cgccgacgtg cgtaccgacg gcgcgatgcg cgcctatgcc
     4141 ggctacgacg ccgagcgggt ggcggcggag gcgcaggcgg cggcgccggg cgcggtcggc
     4201 gacgccggcc tgttcggcgc cggccacatg gccttcacca tcgaccaggg cggcgaccgc
     4261 gaccgctacc agggcctggt cgagctcgag ggcgcgacct tggccgactg cgcgcaggcg
     4321 tatctgacgc gctccgagca gctccgcgcc gacatctcaa ccgcggcggt gcgctggcct
     4381 gagcgcaccg gcggcgccag ctggcgcgcc ggctgcctct tggtccagca ggtgcccgcg
     4441 ggcgacggcc gcggcggcga cgatctcgcg cgcgccgcca actggaccga gacccgggcg
     4501 ctggtcgggc ggctgcgccg cgtcgacctc gccgatccgc tggcggtgcc gccggcgctg
     4561 atcgcgccct tgtttccggg ccgcgacatc cggctgttcg atccgcgccc gctgcgcttc
     4621 gagtgccgct gcacggcggc gcgcgccgcg cgcgtgttgc aggtgcttgg gcccgaggag
     4681 cgcgccgagc tgatgatcga cggccgcatc gaggtcacct gcgactactg caacacgcgc
     4741 tacctgttcg accccgacgg caagctgatc gctgacgcct gaggccggag tttccgctcg
     4801 caggatcact tgccgtcatc gcatcggggc agctcgcgag tcggcaacgg gcggtatagt
     4861 ttcgctttcg gtctatgccc gatcgagtta tcgtttgggt tgcttggaag gggttgtccg
     4921 gcaagtctca gcctacgccg gcggaggggg ggacatgcca cgcctggctg ctgttccgtg
     4981 gcgctctgcg ctgcctgtac tcctgatctt gctgatttta tcgggctgca ccgggcgtac
     5041 cgcgcgccag gcaagcccgg tgctacccga gacgctgccg gccgtggggt cggagctgcg
     5101 gccgcagatg gtggcggtcc ggcggccggt gcagtgtgtg gcctacgccc gggcgctgag
     5161 cggcctgccg atacgtggcg atgcctggac ttggtggccg gcggcagacg gccgctatct
     5221 gcgcaatcgg acgcctcggc cgggcgcctt gttggtcctg gagcggaccc gccgcctacg
     5281 gcacggccat ctggccatgg tcacccatgt gctggggccg cggcaaattc tcgtcgatca
     5341 cgccaactgg ctgaaccaag gccgaatcca tcggcacacg ccggtggtgg atgtgtcgcc
     5401 ggggaacgac tggtcgctgg tgcgggtctg gtatatcccc ggcaaccgcc tgggcagcaa
     5461 cagctatccc gcttacgggt tcgttcaccc cgaggcgttg accgccgcgg caccctctcc
     5521 ggcgaggcgc aacgcctcgt ttccacctgc ccggaaatcg tagccgaggg accggttcgc
     5581 gccggcgggc gcgctggacg cgcgccgacg acatctcgac atgatcgcgc gatcttgtgg
     5641 cgttgatccg gagcaaggcg ttttcccccc gcgtgcccgc agtctatact gcgtccgatg
     5701 acgcccttgc gcctgcccat tgccgtcgtc cttgtcgcgc tcgccgcggc gtgcgcgccg
     5761 ccgcccgccg agccgcgctt ccccgaggtc accttccgcg accaggcgcc gttccgcttc
     5821 gatgccgcga ccgtcgaggt ggtcgaggcg ttccagccgc cgctcaaggt cccccatgtc
     5881 gagcacgagg tgccggtgcc gccggcggcg atgctcaagc gctgggtcgc cgaccgcgtg
     5941 gtcgccgcgg gcgccgaggg gcgggtgcgc gcgaccatcg tcgatgccag catcgtcgag
     6001 acgccgctcg ccaccaacca gaccatcgcc gccggcctga ccaccgagca ggcggtgcgg
     6061 ttcgacgccc gggtcgagct cgagctcgaa tatctggatg cgcgcggcgt gcccaagggc
     6121 gcggtacgcg gcgaggcgcg gcgctcgcgc acggtgcccg aggacgcgac cttgcgcgag
     6181 cgcgacgagg gcctgttcca gatgaccgag gacctggtca acgacctcga ccgcgtgctc
     6241 tcggccaaca tcccgcgcta tctcggcacc cacctggtgc agtgagcgtg ccgcggaagg
     6301 ccggcctgtg cgtgctcgcc gccgtcacat tggcggcgtg cgcgccgctg ccgcccgagc
     6361 tgcaatttcc cgagatcagc ttcgccgacc gggcgccgtt ccgcttcgac gccgcggcgg
     6421 tggaggtggt cgaggcctat cgcccgccgc tcgaatcgcc ccatgtcgag cacgaggcgc
     6481 cggtcgcacc cggcaccgcg ctcaggcact gggtcgacca gcgcgtcgcc gccgccggct
     6541 cgcgtgggcg ggtgcgggtg acgatcgagg acgcaagcat cgtcgagacg ccgctcgcca
     6601 ccaaccggga cctcgaggcg agcctgacca ccgagcgcgc ggcgcgcttc gagggc
//
LOCUS       DASWHU010000027         6652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1857969, whole genome shotgun sequence.
ACCESSION   DASWHU010000027 DASWHU010000000
VERSION     DASWHU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857969"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VGB88_00910"
     CDS             complement(<1..899)
                     /locus_tag="VGB88_00910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018632728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9646025.1"
                     /translation="MTQPAQMRAYGALAGLKVIDLTRILAGPFCTQMLGDHGAEVIKV
                     EPPSGDDTRAWGPPFEGGQSAYFAGVNRNKESLALDLARAEGKAVLLRLLDGADVLVE
                     NFKAGQMERWGLGYEQELKARFPRLVYAEITGFGATGPMAGMPGFDAVAQVMSGLVSI
                     NGAADGAPFRIGVPISDLATGLYAAIAILMALAERTRSGQGQKVEVSLLDCSVSLLHP
                     HAANYLMSGERPRRTGNAHPNISPYDLFATRTAPVFAAIGNDRQFARFTAALGAPALA
                     EDARFRTNADRVINRDALRDALAP"
     gene            1051..2637
                     /locus_tag="VGB88_00915"
     CDS             1051..2637
                     /locus_tag="VGB88_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX9646026.1"
                     /translation="MAAAQREPRGARRGIAVVGADGRLIDCDAAFLAALGRDQIAPGV
                     ALADLVPELAGADFGHRGSLACAGGDGRPAVAVDVRPLDGGAAVLFVAPSAGPPVAGV
                     GPTPERAAFLGMVARSLAGAHAEPAGGVALMIVAVEGGLGLRLRAETAGDPVIAACAE
                     RLRRALGPGRTVAALGGAELAVLFEPGAGESAPEALAQAVIGACAAPFTIDGAEVHLA
                     ARVGVAVAAAGEASAATLMQHADAALGAARIEARPAFRIFDRALGERLREQVALRTEI
                     RRAIARGEFELFYQPVVALDSGRLAGFEALVYWRHPERGLIGPDRFIAAAEAAGLIVP
                     LGNWTLQAACRQLALWRKAGLAGIEAVTIGVNVSGGQFATHELIDHARAALRATGAPA
                     QAITLEITETLLLEDPDFAAEALADLKALDFRLAMDDFGSGHSALGYLLRYPFDTLKV
                     DREFVRGLDRDPQRRAVVRVLALLADALGMTIVAEGVETGAELDALRALGYDYGQGFY
                     FARPLDAAAAERLIAERPTW"
     gene            2667..3659
                     /locus_tag="VGB88_00920"
     CDS             2667..3659
                     /locus_tag="VGB88_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646027.1"
                     /translation="MARELLFGIQTNGIKHSHADPMPDIDTRFRMVKEAGVFDYVDKT
                     PAPDEVEQFLRARDRYGVPVRAGGWFYTLGRDEALFEHNLAVARELGSLVHNTQIMAN
                     HAAGRPVTDAEVVDAYLRFSDLGDEVGVTPCFEVHVNMWSEDFRRVETVARAVEARGA
                     PFNMTLDHSHVIFKIDNPEEQDIGGIRADVEAGRLVLDPFAPGNVCALWIEAGWVRHC
                     HARAAIPNNPKNVDARDAQGRPGRGIQYPFRKPWSGEYHADWDEAALEPWKEVVRALM
                     RHHAGDANGKLGQISTEFIPNLDYGEGCKYSLFEQGIACAKWLRATWAEISAEA"
     gene            complement(3670..4683)
                     /locus_tag="VGB88_00925"
     CDS             complement(3670..4683)
                     /locus_tag="VGB88_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9646028.1"
                     /translation="MTTERPRVSRRRALTIVGAAAGIVLAPALLQGRGARAGPLWTWR
                     GVALGAPARIVLAHPDGPEAERLFEACAGEIERLEAQFSLQRADSALSRLNRDGALAG
                     PDADMVRLMGEAERFARLTDGAFDVTVQPLWRLYAEHFRRQPEDRAGPAPEAIDQALA
                     RVGFGDLEVAPARIAFRRAGMAATLNGIAQGYITDRVTELLRAHGIAQVLVNLGEYRA
                     LGAHPDGRAWRVGLADPFDSTQIAATIDLIDRALATSGGYGTRFSADGRFHHLFDPRT
                     GLSANHHASLTVIADRATVADALSTALYVAPDAVALRVAAALPGIEVHITAADGTLRR
                     LGA"
     gene            4850..6406
                     /locus_tag="VGB88_00930"
     CDS             4850..6406
                     /locus_tag="VGB88_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9646029.1"
                     /translation="MTADVATAEAGRHLGARELLAFFGMTLGMFLAVLNIQIVGSSFA
                     EIQAGLAAGPEEISWVLTAALIAEVIMIPLSGWLSRLLSTRVLFTLSALGFSAASIAC
                     ASAWNIESMIVFRAAQGFSGGAMAPMVFATVFSAFPVRYQTHLIAIVSMLGTGAVALG
                     PSLGGWISEYLSWPWLFLFNVPFGLLAALLVSTLVDYDRPDWRLARRIDFAGIALLAV
                     FFLCLLTVLEEGRREDWLESSKIATLAAVTAVAGVLLLWRELSCAHPVVDLGVFANRN
                     FAIAAFYIMVFGAGLFVPLYFLPLFLARIIGLDTWQIGTWVFVLGLAMMIAGFLAPTL
                     LRVFRRRVVAAAGFALLALGTWLQGRLSADTDFMSLLWPQIIRGFATQLCFLSMVGLA
                     IGLLPVHQVKNGTALFQLTMRLGAAVAVAIANSYLVVRAGVHYHDLRERLPAGHPYAG
                     DVLGVLEGVLGPNLGGAPATDVAGVQMLVRMMEQEAMIRAFNEITLVIALGVAASLAF
                     MPLVRSVRTR"
     gene            complement(6412..>6652)
                     /locus_tag="VGB88_00935"
     CDS             complement(6412..>6652)
                     /locus_tag="VGB88_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9646030.1"
                     /translation="ILAGIKTSAVINVGTATLGALIGAGGYGQPILTGIRLDDTGLIL
                     EGAVPAALLALAVQGAFELAERLVVPRGLTLKAEA"
BASE COUNT          830 a         2353 c         2453 g         1016 t
ORIGIN      
        1 ggcgcgaggg cgtcgcgcag cgcgtcgcgg ttgatcaccc ggtcggcgtt ggtgcggaag
       61 cgcgcgtcct cggcgagtgc gggcgcgccg agcgcggccg tgaagcgcgc gaactggcgg
      121 tcgttgccga tcgccgcgaa caccggcgcg gtgcgggtcg cgaacaaatc gtagggcgag
      181 atgttggggt gcgcgttgcc ggtgcgccgc ggccgctcgc cgctcatcag gtagttggcg
      241 gcgtgcgggt gaaggaggct caccgagcag tcgagcagcg acacctcgac cttctggccc
      301 tgtcctgagc gcgtgcgctc ggccaaggcc attaagatcg cgatcgccgc gtacagcccg
      361 gtggcgaggt cgctgatcgg cacgccgatg cggaacggcg cgccgtcggc ggcgccgttg
      421 atgctgacca gcccgctcat cacctgggcg acggcatcga acccgggcat cccagccatc
      481 ggcccggtcg caccgaagcc ggtgatctca gcgtagacca ggcgcgggaa gcgcgctttc
      541 agctcttgct cgtagccgag cccccagcgc tccatctgcc cggccttgaa gttctcgacc
      601 agcacgtcgg cgccgtccaa gaggcgcagc agcaccgcct tgccctcggc ccgggcgagg
      661 tcgagcgcca ggctctcctt gttgcggttc acccctgcaa agtaggcgct ctggccgccc
      721 tcgaacggcg gcccccaggc gcgggtgtcg tcgccgctcg gcggctcgac cttgatcacc
      781 tcggcgccgt ggtcgccgag catctgggtg cagaacgggc cggcgaggat gcgcgtgagg
      841 tcgatcacct tgaggccggc gagcgcgcca tatgcgcgca tctgggctgg ttgggtcatg
      901 gcggcgcgca gtctggcgtc tgcggcggcc gctgtcagcc cgccggtgtg acaaatttat
      961 tcggaatcgc ttgcgccgcg cccttggtgg catagtggcg cggtgaacag gcaggtcccg
     1021 ccggccccgc accgtcatca atcggaggct gtggcggcgg cgcagcgcga gccgcgcggc
     1081 gcgcgccggg gcatcgccgt ggtcggcgcc gacggcaggc tgatcgactg cgatgcggcg
     1141 ttcctcgccg ccctcggccg cgaccaaatc gcgccgggcg tggcgctggc cgacctggtg
     1201 ccggagcttg ccggcgccga tttcggccat cgcggctcgc tcgcctgcgc gggcggcgac
     1261 ggccggccgg cggtggcggt cgacgtgcgc ccgctcgacg gcggcgccgc ggtgctgttc
     1321 gtcgcgccga gcgcgggccc gccggtcgcc ggcgtcggcc cgacgccgga gcgggccgcg
     1381 ttcctgggca tggtcgcgcg ctcgctcgcc ggcgcccacg ccgagcccgc cggcggggtc
     1441 gcgctgatga tcgtcgcggt cgagggcggg ctcgggctta ggctgcgcgc cgagaccgcc
     1501 ggcgatccgg tgatcgctgc ctgcgccgag cgtctcaggc gcgcgctcgg gcccgggcgc
     1561 acggtcgccg cgctcggcgg cgccgagctt gcggtgctgt tcgagccggg cgcgggcgag
     1621 agcgcgcccg aggccctggc acaggcggtg atcggggcgt gtgccgcgcc gttcaccatc
     1681 gacggcgccg aggttcatct ggcggcgcgg gtgggcgtcg cggtcgccgc cgccggcgag
     1741 gcgagcgcgg cgacgctgat gcagcacgcc gacgccgcgc tcggcgccgc ccgcatcgag
     1801 gcccgcccgg cgtttcgcat cttcgaccgc gcgctcggcg agcgcctgcg cgagcaggtc
     1861 gcgctcagga ccgagatccg ccgcgccatc gcgcgcggcg agttcgagct gttctaccag
     1921 ccggtggtcg cgctcgactc gggccgcctc gccggcttcg aggcgctggt ctactggcgc
     1981 catcccgagc gcgggttgat cggccccgac cggttcatcg ccgccgccga ggccgccggg
     2041 ctgatcgtgc cgctcggcaa ctggacgctg caggccgcgt gccgccagct cgcgctttgg
     2101 cgcaaggccg ggctcgccgg catcgaggcg gtgactatcg gggtcaacgt gtcgggcggc
     2161 cagttcgcca cccacgagct gatcgaccac gcgcgcgccg cgctccgcgc caccggcgcg
     2221 ccggcgcagg cgatcacgct cgagatcacc gagaccctgc tgctcgaaga tcctgacttc
     2281 gcggccgagg cgctcgccga cctgaaggcg ctcgacttcc gcctggcgat ggacgatttc
     2341 ggttccggcc actcggcgct cggttacttg ctgcgctacc cgttcgacac gttgaaggtc
     2401 gaccgcgagt tcgtgcgcgg gctcgaccgc gacccgcagc ggcgcgcggt ggtgcgcgtg
     2461 ttggcgctgc tcgccgatgc gctcggcatg accatcgtcg ccgagggggt cgagaccggt
     2521 gccgagctgg acgcgctgcg cgcgcttggc tacgactacg gccagggctt ttatttcgcc
     2581 cgcccgctcg atgccgcggc cgccgagcgg ctgatcgccg agcggccgac ctggtaggcc
     2641 ggcgcgggcg aacggaggag ggagcggtgg cgagggagct tctgttcggc atccagacca
     2701 acggcatcaa gcacagccac gccgatccga tgcccgacat cgacacccgc ttccgcatgg
     2761 tcaaggaggc gggcgtgttc gactatgtcg acaagacccc ggcgccggac gaggtcgagc
     2821 agttcctgcg cgcccgcgac aggtacggcg ttccggtccg cgccggcggc tggttctaca
     2881 cgctcgggcg cgacgaggcg ctgttcgagc acaatctcgc ggtcgcgcgc gagctcggct
     2941 cgctggtcca caacacccag atcatggcca accacgccgc gggccgcccg gtgaccgacg
     3001 ccgaggtggt cgacgcttac ctgcgcttct ccgaccttgg cgacgaggtc ggcgtgacgc
     3061 cctgcttcga ggtccacgtc aacatgtggt ccgaggactt ccgcagggtc gagacggtcg
     3121 cgcgcgcggt cgaggcgcgg ggcgcaccct tcaacatgac cctcgaccat agccacgtca
     3181 tcttcaagat cgacaacccg gaagagcagg acatcggcgg catccgcgcc gacgtcgaag
     3241 cgggcaggct ggtgctcgac ccgttcgcgc ccggcaatgt ctgcgcgctg tggatcgagg
     3301 cgggctgggt gcgccactgc catgcgcgcg cggcgatccc caacaacccg aagaacgtcg
     3361 atgcgcgcga cgcacagggc cggcccgggc gcggcattca gtacccgttc cggaagccat
     3421 ggtccggcga gtaccacgcc gactgggacg aggcggcgct cgagccgtgg aaggaggtgg
     3481 tgcgcgcgct gatgcgccac cacgccggcg atgcaaacgg caagctcggc cagatttcga
     3541 ccgagttcat ccccaacctc gattacggcg agggctgcaa gtactcgctg ttcgagcagg
     3601 gcatcgcctg cgccaagtgg ctgcgcgcga cctgggccga aattagcgcc gaggcttaag
     3661 cgccgcggct taggcgccga ggcgccgcag cgtgccgtcg gcggcggtga tgtgcacctc
     3721 gatgccgggc agggcggcgg cgacgcgcag ggcgactgca tcgggcgcga cgtagagcgc
     3781 ggtcgacagc gcatccgcaa ccgtcgcccg gtcggcgatg acggtgagcg aggcgtggtg
     3841 gttggcgctc aatccggtgc gcgggtcgaa caggtggtgg aagcggccgt cggcgctgaa
     3901 ccgggtgccg tagccgcccg aggtggcgag cgcgcggtcg atcaggtcga tggtcgccgc
     3961 gatctgggtc gaatcgaacg ggtcggcgag cccgacccgc cacgcccgcc cgtcgggatg
     4021 ggcgccgagc gcgcggtact cgcccagatt gaccagcacc tgggcgatgc cgtgcgcgcg
     4081 caacagctcg gtgacgcggt cggtgatgta gccctgggcg atgccgttca aggtcgccgc
     4141 catgccggcg cggcggaacg cgatgcgcgc cggcgcgacc tcgaggtcgc cgaagccgac
     4201 ccgcgccaag gcctggtcaa tcgcttcggg cgcgggcccg gcgcggtcct ctggctggcg
     4261 ccggaagtgc tcggcgtaga gccgccacag cggctgcacg gtgacatcga acgcgccgtc
     4321 ggtgagccgg gcgaagcgct ccgcctcgcc catcagccgg accatgtcgg cgtcgggccc
     4381 ggcgagcgcg ccgtcgcggt tgaggcggga gagcgcgctg tccgcgcgct gcaggctgaa
     4441 ctgcgcctcg aggcgctcga tctcgcccgc acaggcctcg aacagccgct cggcctcggg
     4501 cccgtcgggg tgggcgagca cgatccgcgc cggcgcgccg agcgccaccc cgcgccaggt
     4561 ccacagcggg cccgcgcgcg cgccgcggcc ctgcaacagc gccggcgcca gcacgatgcc
     4621 cgcggccgcg ccgacgatgg tcagcgcgcg ccggcgcgag acgcggggac gttcagtcgt
     4681 catgtcgggc cgggcctttg cctcggttcg ggtctcggtt cgtgtcaggt agcctatcat
     4741 ccagattgcg cgcgccgcgt ccagccgcgc aacgccagcc ctcggatgct accttgcccc
     4801 ggggcggcgc ccggcggcgc ggccagggac aggatgtcgg cgaggcgcca tgaccgcgga
     4861 cgttgcgaca gccgaggccg ggcgccatct cggcgcgcgc gagctgctgg cgttcttcgg
     4921 catgaccttg ggcatgttcc tggcggtgct caacatccag atcgtcggca gctcgttcgc
     4981 cgagatccag gccgggcttg cggccgggcc cgaggagatc agctgggtgc tgaccgcggc
     5041 gctgatcgcc gaggtgatca tgatcccgct gtcgggatgg ctgtcgcggc tgctgtcgac
     5101 gcgggtgctg ttcaccctgt ccgcgctcgg cttcagcgcc gccagcatcg cctgcgccag
     5161 cgcctggaac atcgagtcga tgatcgtgtt ccgcgccgcc caggggttca gcggcggcgc
     5221 catggcgccg atggtgttcg ccaccgtgtt ctcggcgttt ccggtgcgct accagaccca
     5281 cctgatcgcg atcgtctcga tgctcggcac cggcgcggtc gcgctcgggc cgagcctcgg
     5341 cggctggatc agcgagtacc tgtcgtggcc gtggctgttc ctgttcaacg tgccgttcgg
     5401 gctgctcgcg gcgctgctgg tctcgaccct ggtcgactac gaccgtcccg actggcggct
     5461 ggcgcgccgc atcgacttcg ccggcatcgc gctgctggcg gtgttcttcc tgtgcctgct
     5521 gaccgtgctc gaggagggcc ggcgcgagga ctggctcgag tcctcgaaga tcgccacact
     5581 cgccgcggtc accgcggttg ccggcgtgct gctgttgtgg cgcgagctca gctgcgccca
     5641 tccggtggtc gacctcggcg tgttcgccaa ccgcaacttc gccatcgccg cgttctacat
     5701 catggtgttc ggcgccggcc tgttcgtgcc gctctatttc ctgccgctgt tcctcgcccg
     5761 catcatcggc ctcgatacct ggcagatcgg cacctgggtg ttcgtgctcg gcctggcgat
     5821 gatgatcgcc ggtttcctgg cgccgaccct cttgcgcgtg ttccgccggc gcgtggtcgc
     5881 ggccgccggc ttcgcgctgc tcgccctcgg cacctggctg caaggccggc tgtcggcgga
     5941 taccgacttc atgtcgctct tgtggccgca gatcatccgc ggcttcgcta cccagctctg
     6001 cttcctgtcg atggtcgggc tcgccatcgg cctgttgccg gtccaccagg tcaagaacgg
     6061 caccgcgctg ttccagctga ccatgcggct cggcgcggcg gtggcggtgg cgatcgccaa
     6121 cagctatctg gtggtccgcg ccggcgtcca ttaccacgat ttgcgcgaac ggctgccggc
     6181 cggccaccca tacgccggcg acgtgctcgg ggtattggag ggcgtgctcg gccccaacct
     6241 cggcggcgcg ccggcgaccg acgttgccgg cgtccagatg ctggtccgga tgatggagca
     6301 ggaagccatg atccgggcgt tcaacgagat cacgctggtg attgcgctcg gtgtcgccgc
     6361 ctcgctggcg ttcatgccgc tggtgcgcag cgtgcgcacg cgctgagcgc ctcaggcctc
     6421 ggccttaagg gtcaggccgc gcggcaccac caggcgctcg gcaagctcga acgcgccctg
     6481 gacggcgagc gcgagcagcg ccgccggcac cgcgccctcg aggatcaggc cggtgtcgtc
     6541 gaggcggatg ccggtcagga tcggctggcc gtagccgccg gcgccgatca gcgcgccgag
     6601 ggtggcggtg ccgacattga tcaccgccga ggtcttgatt ccggccagga tc
//
LOCUS       DASWHU010000028         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1117537, whole genome shotgun sequence.
ACCESSION   DASWHU010000028 DASWHU010000000
VERSION     DASWHU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117537"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGB88_00940"
     CDS             complement(<1..522)
                     /locus_tag="VGB88_00940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9646031.1"
                     /translation="MPAYRSHRTTQGAAQAAARALWRATGTAEGDLDKPIVAVANSFT
                     QFVPGHVHLKDLGQLVAREVKKAGAVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIAD
                     AVEYMVNAHCADALVCISNCDKITPGMLMAALRLNLPTIFVSGGPMEAGKMGGDGGAI
                     IDVVDALVAGTEPD"
     gene            complement(662..1009)
                     /gene="grxD"
                     /locus_tag="VGB88_00945"
     CDS             complement(662..1009)
                     /gene="grxD"
                     /locus_tag="VGB88_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX9646032.1"
                     /translation="MTTTHTSALNERIEKELSSHPVVLFMKGTPIFPQCGFSALVVQV
                     LSHVGVKFKSIDVLSEPDIREGIKAYSNWPTIPQLYVKNEFVGGADIVREMYESGELV
                     ELLNSRGIEVTPS"
     gene            complement(1052..1291)
                     /locus_tag="VGB88_00950"
     CDS             complement(1052..1291)
                     /locus_tag="VGB88_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX9646033.1"
                     /translation="MAMNASEIERLIRAALPDADVTIEDLRGDGDHYAAFVSSPAFKG
                     KTRVQQHQMVYDALKGNVGGALHALALQTAVPEDA"
     gene            complement(1320..>1695)
                     /locus_tag="VGB88_00955"
     CDS             complement(1320..>1695)
                     /locus_tag="VGB88_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX9646034.1"
                     /translation="ERRNGDFVRALIAAGRVSCCHDLSDGGLYVALAEMAIAGGVGAD
                     LAGPEDDAWLFGEDQARYLLAVEAAETGAVLAEAEAAGVPAQRVGTTGGVALTRSGAD
                     AISVGELRSLHASWLPAYMARA"
BASE COUNT          239 a          604 c          582 g          270 t
ORIGIN      
        1 gtcgggctcg gtaccggcga ccagcgcgtc gaccacgtcg atgatcgcgc cgccgtcgcc
       61 gcccatcttg cccgcctcca tcggcccgcc ggagacgaag atcgtcggca ggttgagcct
      121 gagcgcggcc atcagcatgc ccggcgtgat cttgtcgcag ttcgagatgc acaccagcgc
      181 atccgcgcag tgggcgttga ccatgtactc gaccgcgtcc gcgatcagct cgcgcgaggg
      241 caggctgtag agcatgccgt catggcccat ggcgatgccg tcgtcgaccg cgatggtgtt
      301 gaactccttg gccaccgcgc ccgccttctt gacctcgcgc gcgaccagct ggccgaggtc
      361 cttgaggtgg acgtggccgg gcacgaactg ggtgaacgag ttggcgaccg cgacgatcgg
      421 cttgtcgaga tcgccctcgg cggtgccggt ggcgcgccac agcgcgcgcg ccgcggcctg
      481 ggcggcaccc tgggtggtgc gatgcgaacg ataggcgggc acggtgctct cctcaggtaa
      541 cggtcggcgc ccctgtctcg taccggcagc cgaaaaaatc aagggcggcg cggcgcggcg
      601 ctccaaccaa aaaggccgcc cggtgggggc ggccttccgg acttggagac ttgcgccgcg
      661 ctcaggacgg cgtgacctcg atgccgcggc tgttgagcag ctcgaccagc tcgcccgact
      721 cgtacatctc gcgcacgatg tcggcgccgc ccacgaactc gttcttgacg tagagctgcg
      781 ggatcgtcgg ccagttgctg taggccttga tgccctcgcg gatgtcgggc tcggacagca
      841 cgtcgatgct cttgaacttg acccccacgt ggctcagcac ctggaccacc agcgccgaga
      901 agccgcactg gggaaagatc ggcgtgcctt tcatgaacag caccaccgga tgggacgaga
      961 gctccttctc gatccgctcg ttcagcgcgc tggtgtgtgt cgtcgtcatc gcggtgtctc
     1021 ctggctggat tggtagccgc cggcgccggg ctcacgcgtc ctcgggcacg gcggtctgca
     1081 gcgccagcgc gtgcagcgcg ccgccgacgt tgcccttgag cgcatcatag accatctggt
     1141 gctgctggac gcgggtcttg cccttgaacg ccggcgagct gacgaacgcc gcgtagtggt
     1201 cgccgtcgcc gcgcaggtcc tcgatggtga cgtcggcgtc gggcagcgcg gcgcggatca
     1261 gccgctcgat ctcgctcgcg ttcatcgcca tcaagtcggc tcctcggcgg ccttcggcct
     1321 cacgcccggg ccatgtaggc gggcagccac gatgcgtgca gcgaccgcaa ttcgcccacc
     1381 gatatggcat ccgcgccaga gcgtgtcaat gcgaccccgc cggtggtgcc gacccgttgg
     1441 gcgggcacgc cggcggcctc ggcctcggcc aggaccgcgc cggtttccgc cgcctcgacc
     1501 gccagcaggt agcgcgcctg gtcctcgccg aacagccagg cgtcgtcctc gggcccggcc
     1561 aggtcggcgc cgactccgcc cgcgatcgcc atctcggcga gcgccacgta gagcccgccg
     1621 tccgacaggt cgtggcagca ggaaacccgc ccggccgcga tcagcgcgcg cacgaagtcg
     1681 ccgttgcgcc gctcg
//
LOCUS       DASWHU010000029         5085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1858370, whole genome shotgun sequence.
ACCESSION   DASWHU010000029 DASWHU010000000
VERSION     DASWHU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858370"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..2215
                     /locus_tag="VGB88_00960"
     CDS             275..2215
                     /locus_tag="VGB88_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX9646035.1"
                     /translation="MRSSRRVVAWSAVVIAALLFIALNVLSQTLLRSARLDLTEGRLY
                     TLSDATRAVLSGLGEPITVRFFFSESLAADLPTISQYARRVQELLETYADESAGKITL
                     QVIEPEPFSEAEDEAVAFGLQGVPVSNAGDRVYFGMAATNTTDDQEIIPFFQDQREAF
                     LEYDLTRAFYKLAQPKRPVVGLISTLPLAGGFDPAQGFSQPWVVAQQIQQLFELKDLS
                     INVAAVPDDVGVLMVVHPKDLPEGTRYAIDQYVLKGGHAMVFVDPFSEVAAGRPDPQD
                     PVRLHNSSLPELFEAWGVKLEPGMVVGDRMAARRVRTGPRDRPEAVDYVAWLALDQSH
                     MNQDEVVTSQLGVINLATAGALTPVEGATTSFVPLLTSSPVSELIERLQVQFVPDPKK
                     ILAEFKPTGAQYVMAARVTGPVKSAFPDGEPGKGDADQDAGADAGEVAAEPEPGGHLS
                     ESAGPVNLILVADSDLLDDRTWVQVQDFLGRSFAVPAADNGTMVVNALDVLAGSSDLI
                     SLRSRGTSARPFVVVEDLERQAEDRFRETEQTLQQRITDTEKRLAELQTGAGEGAGAA
                     GARVVLSPEQSQAIGEFREQLVATRKQLRDVQLALRRDIESLGTLLKFLNIALVPLII
                     GVLAVAISVVRRQRRKRVMGMA"
     gene            2259..3515
                     /locus_tag="VGB88_00965"
     CDS             2259..3515
                     /locus_tag="VGB88_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX9646036.1"
                     /translation="MKTRNLTILAIVTAVVVIIAGAAVVWRQSESRRTVVESALFPGL
                     IDRLDDVQAIEITAGADSFRIARAGENWVVPAKADFPARFDLVRKALIQIGELKTIEA
                     KTADPERHPDLGLAAPAADVEGAGRRVRLLGADDAELAALVVGLRARGARDMVYVRRA
                     GDDQAWLARSEIDPGAKPMDWIDSLVVKLPFERIRGGVIRHADGEVVEFSKDVPDATA
                     FELADLPEGAKVASALGIDSLARAIAMLRFDDVAARENAPVTGEPAAVDSYRTFDGMT
                     VEVKAWANAAAGEEPDAMPTFWVEIAASYDPAAAAPAPAPDSGASEQDQDEADAEGEE
                     EEPFDPAAETERMVARTGRWAFQVPDFKGEQFTMRRADLIEMPTPEPATAEGGGADEG
                     GAAAPPEPGAGAAETPLEGDGEPTTR"
     gene            3565..4260
                     /locus_tag="VGB88_00970"
     CDS             3565..4260
                     /locus_tag="VGB88_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9646037.1"
                     /translation="MTDRAPIAIDYFSDLLCIWAYVAQARLDEVRRRFAERVLVRARF
                     CPIFGDTARRIGEGWRERGGYDGFNAHVREVAGRFDHVAVHPRLWLDVRPASSAGAHL
                     YVKAMQVAEAGAAHDDGGAPPASDRLAWAFRLAFFRDCRDIARLEIQDDVARGLGLPL
                     AAIHDAIASGQAHAALADDLEARDALRIEGSPTLVLNDGRQKLYGNVGYRVIEANIQE
                     LLKEPDAGQASWC"
     gene            4446..4661
                     /locus_tag="VGB88_00975"
     CDS             4446..4661
                     /locus_tag="VGB88_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646038.1"
                     /translation="MAASIAGGYMLHAAVERTRATSGLDQGTSAVSAVVRGWFNVDPI
                     LAAILAPAVIVAVLSLGAMCIWARTDR"
     gene            4714..4893
                     /locus_tag="VGB88_00980"
     CDS             4714..4893
                     /locus_tag="VGB88_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646039.1"
                     /translation="MWTYLLAALLGAGIGLATGDLIFPTAHAHLGIELFNYAKALGGG
                     AIGLWAWALAQRQRV"
     gene            complement(4912..4987)
                     /locus_tag="VGB88_00985"
     tRNA            complement(4912..4987)
                     /locus_tag="VGB88_00985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4952..4954),aa:Thr,seq:tgt)
BASE COUNT          782 a         1756 c         1811 g          736 t
ORIGIN      
        1 ctgttcgcca acgtggtcgc ggtcgagcac atcaaggcgg cctagagcgc gatgatatta
       61 agtcggatcc ctgagcctgt tttggtcgtc accccggact ccgcgaagcg gatatccggg
      121 gtccatacgc ctttgccacg cgctcgacca cccgtgcaag cggcgggatg gaccccggct
      181 cgcggcgccg cgcgccttgg ccggcgtgac gggtgatgga tcaccatgtt tcaaatcatc
      241 aggctctagg cgtgtccgcg cgcaggggga ggcaatgagg tcatcgcgca gggtcgtcgc
      301 ctggtcggcg gtcgtgatcg cggcgctgct gttcatcgcg ctcaacgtgc tcagccagac
      361 cctgttgcgc agcgcccgcc tggaccttac cgagggccgg ctgtacacgc tctcggacgc
      421 gacccgcgcg gtgctgtccg gcctcggcga gccgatcacg gtgcggtttt tcttcagcga
      481 gtcgctggcc gcggacctgc cgaccatcag ccagtacgcg cgcagggtcc aggagttgct
      541 cgagacctat gccgacgagt ccgcgggcaa gatcacgctt caggtgatcg agcccgagcc
      601 gttctcggag gccgaggacg aggcggtggc gttcgggctt cagggcgtgc cggtgagcaa
      661 cgccggcgac cgcgtctact tcggcatggc ggcgaccaac accaccgacg accaggaaat
      721 catcccgttc ttccaggacc agcgcgaggc gttcctcgag tacgacctga cgcgcgcgtt
      781 ctacaagctc gcccaaccca agcgcccggt ggtcggcctg atcagcaccc ttcccctggc
      841 cggcggcttc gaccccgcac aaggcttcag ccagccctgg gtggtggccc agcagatcca
      901 gcagctgttc gagctcaagg acctcagcat caacgtcgcc gcggtgcccg acgacgtcgg
      961 cgtgctgatg gtggtccacc ccaaggacct gcccgagggg acccgctacg ccatcgacca
     1021 gtacgtgctc aagggcgggc atgcgatggt gttcgtcgac ccgttctccg aggtcgccgc
     1081 cggccggccc gatccccagg atccggtgcg gctgcacaac tcgagcttgc ccgagctgtt
     1141 cgaggcgtgg ggggtgaagc tcgagccggg catggtggtc ggcgaccgca tggccgcccg
     1201 gcgcgtgcgc accggcccgc gcgaccgccc cgaggcggtc gactacgtgg cctggctggc
     1261 gctcgaccag agccacatga accaggacga ggtggtgacc agccagctcg gggtgatcaa
     1321 tctcgccacc gccggcgcgc tgaccccggt cgagggcgcg accaccagct tcgtgccgtt
     1381 gctcacttcc tcgccggtgt ccgagctgat cgagcggctg caggtgcagt tcgtgcccga
     1441 cccgaagaag atactggccg agttcaagcc gaccggcgcg cagtacgtga tggcggcgcg
     1501 ggtcaccggc ccggtcaaga gcgccttccc ggacggtgag ccgggcaagg gcgatgccga
     1561 ccaggatgcc ggcgcggacg cgggcgaggt tgcggccgag cccgagccgg gcggccacct
     1621 gagcgagtcg gcgggacccg tgaacctgat cctggtcgcc gattccgacc tgctcgacga
     1681 ccgcacctgg gtccaggtcc aggacttcct ggggcggtcg ttcgccgtgc cggcggccga
     1741 caacggcacc atggtggtca acgcgctcga cgtgctggcg ggctcgagcg acctgatcag
     1801 cctgcgcagc cggggcacct cggcgcggcc gttcgtggtc gttgaggacc tggagcggca
     1861 ggcggaggat cgcttccgcg agaccgagca gacgcttcag cagcgcatca ccgacaccga
     1921 gaagaggctc gccgagctgc agaccggcgc cggcgaaggc gccggcgcgg ccggcgccag
     1981 ggtggtgctg agccccgagc agagccaggc gatcggcgag ttccgcgagc agctggtggc
     2041 gacccgcaag cagcttcgcg acgtccagct ggcgctgcgc cgcgacatcg agtcgctcgg
     2101 caccctgctc aagttcctca acattgccct ggtgccgctg atcatcggcg tgctcgcggt
     2161 ggccatctcc gtggtccgcc ggcagcggcg caagcgggtc atgggcatgg cctgagacgc
     2221 cccggcgcgg cgcggcacaa ccacacgaaa ccgagtccat gaagaccaga aacctgacta
     2281 tcctcgccat cgtcaccgcc gtggtcgtga tcatcgccgg tgcggcggtg gtctggcgcc
     2341 agtccgagag ccggcgcacc gtggtcgaga gcgcgctgtt ccccggcctg atcgaccggc
     2401 tcgacgacgt ccaggcgatc gagatcaccg ccggcgccga cagcttccgc attgcgcgcg
     2461 cgggcgagaa ctgggtggtg ccggccaagg ccgacttccc ggcgcggttc gacctggtgc
     2521 gcaaggccct gatccagatc ggcgagctga agacgatcga ggccaagacc gccgatcccg
     2581 agcggcaccc tgatctgggc ctcgccgccc ccgccgccga cgtcgagggc gcgggccggc
     2641 gcgtgcgcct gctcggcgcc gacgatgccg agctcgccgc gctggtggtc ggcctgcgcg
     2701 cgcgcggcgc ccgcgacatg gtctatgtgc gccgggccgg cgacgaccag gcttggctcg
     2761 cgcgctccga gatcgacccc ggggcgaagc cgatggactg gatcgacagc ctggtggtca
     2821 agctgccgtt cgagcgcatc cggggcgggg tcatccgcca cgccgacggc gaggtggtcg
     2881 agttctccaa ggacgtcccg gacgcgaccg cgttcgagct ggccgacctg ccggaagggg
     2941 ccaaggtggc ctcggcgctc ggcatcgaca gcctggcgcg cgcgatcgcg atgctgcgct
     3001 tcgacgacgt cgccgcccgc gaaaatgcgc cggtcaccgg cgagccggcg gcggtggaca
     3061 gctatcggac cttcgacggc atgaccgtcg aggtcaaggc gtgggccaat gccgcggcgg
     3121 gcgaggagcc cgacgccatg cccacttttt gggtggagat cgcggccagc tacgacccgg
     3181 ccgccgccgc gccggcaccg gcgccggact ccggggcgag cgaacaggac caggacgagg
     3241 cggatgccga gggggaagag gaggagccgt tcgaccccgc ggccgagacc gagcggatgg
     3301 tcgcgcgcac cggccgctgg gcgttccagg tgcccgactt caagggcgag cagttcacca
     3361 tgcgccgcgc cgatctgatc gagatgccga cacccgagcc cgccaccgcc gagggcgggg
     3421 gagccgacga aggcggcgcc gcggcgccgc ccgagccggg cgccggcgcg gccgagacac
     3481 cgctcgaagg cgacggcgag ccgacgacgc ggtagcttcc cgcgcccggc gccggcggca
     3541 ggtgagcggc gagggagggc gggcatgacc gatcgggcac cgatcgcgat cgattacttc
     3601 tcggacctgt tgtgcatctg ggcctatgtc gcccaggcgc gcctcgacga ggtgcgccgc
     3661 cggttcgccg agcgcgtgct ggtgcgcgcc cggttctgcc cgatcttcgg cgacaccgcc
     3721 aggcggatcg gcgagggctg gcgtgagcgc ggcggctacg acgggttcaa tgcccatgtg
     3781 cgcgaggtcg ccgggcgctt cgaccacgtc gcggtccacc cgcgcctgtg gctcgatgtg
     3841 cggccggcgt cctcggccgg cgcccatctc tacgtcaagg cgatgcaggt cgccgaagcg
     3901 ggggcggcgc acgacgacgg cggcgccccg ccggcctccg accggctggc ctgggcgttc
     3961 aggctcgcct tcttccgcga ctgccgcgac atcgcccggc tcgagatcca ggacgacgtc
     4021 gcccgcggtc tcggcctgcc gctcgcggcg atccacgatg cgatcgccag cggccaggcg
     4081 cacgcggcgc tggccgacga cctcgaggca cgcgatgcgc tgcgtatcga gggcagcccg
     4141 accctggtgc tcaacgacgg ccgccagaag ctgtacggca acgtcggcta ccgggtcatc
     4201 gaggccaaca tccaggagct gctgaaggag cccgacgccg gccaggcaag ttggtgctga
     4261 gtcattcgcc gccgccggcg acgcaaaaga agtgttgcag cggtcgcaag attttactat
     4321 tgtccttagg gaaatcccta agaaggatgt gcctggaaaa ggcaccgacg accatgcaag
     4381 cgctaagaca gaagattaga aattttgcag tatccattag caagtatgcg cttttgctca
     4441 ctgctatggc cgccagcatc gccggcggct acatgctaca tgccgccgtc gagcggaccc
     4501 gggccacatc cgggctcgac cagggcacct cggccgtgag cgcggtggtg cggggctggt
     4561 tcaacgtcga cccgatcctc gccgcgatcc tggctccggc cgtgatcgtc gccgtgctgt
     4621 cgctcggcgc gatgtgcatc tgggcgcgca ccgaccggtg acgcgcggtt cggcgacggg
     4681 cactgcagag gggcataagc gcggagtgcg gccatgtgga cttacctgct ggcagcgctg
     4741 ctgggcgcgg ggataggact cgccaccggc gacctgatct tccccaccgc tcacgcgcat
     4801 ctcggaatcg agctgttcaa ctacgccaag gccctcggcg gcggcgcgat cggcctctgg
     4861 gcctgggcac tggctcagcg ccaaagggtg tgacccgcgg ccactggcgg ctggtgccgg
     4921 ttgcaggatt cgaacccgcg accccctgat tacaaatcag gtgctctacc agctgagcta
     4981 aaccggcccg accgcgggcg ggcggcccgc cgccgcataa gcgtaagatg gcatgatggc
     5041 acggcggcga gtggattttt tccgccgcat agtccgtttg catca
//
LOCUS       DASWHU010000030        16254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2598390, whole genome shotgun sequence.
ACCESSION   DASWHU010000030 DASWHU010000000
VERSION     DASWHU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16254
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598390"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1586)
                     /locus_tag="VGB88_00990"
     CDS             complement(45..1586)
                     /locus_tag="VGB88_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9646040.1"
                     /translation="MAPRTPAPGGARPLLRGSVGLWAAVAVVLIAASCNSHGLSSRPL
                     GFSHASFDSTAKDYPELAEVSAAFDRNVAPRPDADLRQRQLDLFAEVLDITRGDYVEP
                     VAMPHMIGLAVEGFDQVGIEADLRERADAPATDAEDEDDELTADNLMSAGLDRMLSKL
                     DSHSGYLSPERYREMKLRTRGEFGGIGIEVTVEDGLVKVVAPIDESPGQRAGLQAGDL
                     ITHVDGTSVFGMSLEQAVNLMRGPAGSRVVLELRRPPATETFRVAIVRDTVRIQAVRA
                     HAEGDIGYVRITTFNEQAEEALRRAVKDLRGELGKRMAGLVIDLRNNPGGLLDQALGV
                     SDAFLSGGEIVSTRGRERNTVRRFSAEGGDIADGLPIVVLINGGSASASEIVSGSLQD
                     HQRAVVIGTRSFGKGSVQTIIPVSGGGAVRLTTARYFTPSGRSIQLSGIVPDIVVGVD
                     EAGMRREADLENPLPSDSADVTGTAKLTADQACPTSGQREDPILACAVELLESRATVA
                     AGLSN"
     gene            complement(1586..2380)
                     /locus_tag="VGB88_00995"
     CDS             complement(1586..2380)
                     /locus_tag="VGB88_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9646041.1"
                     /translation="MLESARGVMGGGRAARAAAAIAVVVAALALGWATAVPPARADEA
                     LSDAQQAEVEALIERYLHEHPEVILDSVRALRDREETSARERAQTNLIALGDQIRYDP
                     ATPVAGNPDGDVTVVEFFDYRCGYCKRSLDTVMALIKSDPNIRMVFKEFPILTPESER
                     ASRAALASVSQGKYLQFHYALMSSRGTLSDRAIMDIAAEVGLDVERLAQDMESAAIAA
                     QIDANHALARELDINGTPTFIVEDRMFPGAVDAEVLERAIAERRSG"
     gene            complement(2560..3906)
                     /locus_tag="VGB88_01000"
     CDS             complement(2560..3906)
                     /locus_tag="VGB88_01000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9646042.1"
                     /translation="MAAAQVAATTAWAQNLPLIRDSEIEDTIRGWADPLFVAAGLNPA
                     DIRILLVNDEALNAFVAGGQNLFIHTGLLTATDFPGQLIGVIAHETGHIAGGHLARAG
                     DAIREAQNLGLFATLLGIGLIAVGAASGAATARAGGAVIAGGQSVGLRTFLAYTRAQE
                     SAADQAALNYLDRTKQSAAGLLSFLERLQDQELLVTSRQDPYVRTHPLSRDRIEHIAE
                     HVRASRWTSQAASEAEMERHARMKAKLIGYLERPQRVMKSFYPETDTSLAARYARAVA
                     YLRLPDRDKALAEIDSLIAERPDDPYFVELKGQIMFEHAKLREAVTYYQQAVNMRPDD
                     PLLLTGLGQAQVETGDASLVAPAIENLERANRRDHDNPSTWRLLATAYHQVGDVGASS
                     LAAGEYALLIGQVENASVHAGRAEREYAEGTPGWLRAQDIKTEAEQLKALQERRER"
     gene            4046..5191
                     /locus_tag="VGB88_01005"
     CDS             4046..5191
                     /locus_tag="VGB88_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9646043.1"
                     /translation="MTLKASERSAIAPFYAMEVLRAANRRAAEGAHVLHLEVGEPGAG
                     APEPVLAAARAAIGRVRLGYTEALGTPALRARIARHYRDRYALAVDPARVAVTIGASG
                     AFLLAFLAAFDVGDRVAVAEPCYPAYRNILSALGIEVVALQAGPEHRFQPTVELLDQA
                     GPLDGLVVASPSNPTGTMLAPDQLARLAGWCEAHGVRLISDEIYHGITYGMAESCALA
                     HSERALVVNSFSKYFAMTGWRLGWMVMSEDVAGAVDRLAQNLFVSPAALPQHAALVAF
                     DCRDQLDAYVAGYARNRKMLLERLPEAGFDRLAPADGAFYLYADVSRLANDSQELCAR
                     MLDEIGVAATPGVDFDPRRGHRYVRFSFAGGEAEIAAAAERIVAWRP"
     gene            complement(5220..7616)
                     /locus_tag="VGB88_01010"
     CDS             complement(5220..7616)
                     /locus_tag="VGB88_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX9646044.1"
                     /translation="MAKRMLIDATHTEETRVVVVDGKKLEEFDFESSTKRQLKGNVYL
                     AKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQIPVSDRKALIAQQRKEAAADDD
                     ERGAPREPAAAEDDDAEPVETVSGQEQEEIARRPATLPRYRIQEVIRRRQILLVQVTK
                     EERGTKGAALTTYISLAGRYCVLMPNTTRGGGISRKIQSGEARKRLRSVVASLSLSEG
                     MAVILRTAGQERTKAEIRRDFEYLMRLWDEIRESTLKATAPALIHEEANLIRRSIRDL
                     YDSKMVEVLVEGDEGYRVAKDFMRTLMPSHAKRVQPYKDGLPLFTRYQVDSQLDTMHN
                     ATVELPSGGYVVFDPTEALVAIDVNSGRSTRERNIEETATATNLEAADEIARQLRLRD
                     LAGLIVIDFIDMEEHRNVRAVEKRLKEAVQSDRARIQMGRISSFGLLELSRQRLRPSL
                     LEASSVNCQHCAGTGRVRSVESIALHALRVIEDEGIRGRAAGIQATMPSPVALYLLNN
                     KRAALAEIESRYGISVRIATDDSPAPPTCRIELVTPRTEPVVVPAVLAEAVAAEMTAE
                     TAEKVAEEPGRRRRRRRAEEPARPAGAEADEAEAAEEEAEAETAEFAGEAEAEAEAGE
                     GEARPRRRRRGRRGGRRRSRTRETEAAAEPQHATADGGEPGAAEPAGEAEGAVEPVAA
                     ADAEASIVKPKRRRRTRKTARTGDGEAGESAPVAAAALQAVEPVAASAPEAADGGRAP
                     TGNGANGGHRAEVRPAQQAVAFAGPAAGGGESGAEGKAEPDDDDTARRRGWWQRLTGV
                     "
     gene            8168..9451
                     /locus_tag="VGB88_01015"
     CDS             8168..9451
                     /locus_tag="VGB88_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9646045.1"
                     /translation="MLPVDALTDNADARPSETSTAVSGGRHARFALACAVGLVVASGL
                     AGAAHAVPEASEARAWRHPDYTRLVIELSEQVEFSIFTLAEPRRMVVDFPELDFRLPP
                     RPFGDGAVGVIATMRYGLFEPGVSRLVLDLARPARVRDSFLMPPSEGKPYRFVLDLEE
                     TDAADFAAAAQANPPPRPVPGPVIEPPAATVAPRSDGKRVIVVDAGHGGVDPGAISAS
                     GLYEKQIALVAARELAERLQATGRYQVVMTRDRDIFLSLRSRVSVARRAGADLFVSLH
                     ADSIADKGVRGSHVYSLSKTASDAAAAALAAAENKSDVIAGLDLAEYTPEVHTILLDL
                     SQRETNNTSAEVASRLVDELQRNGIRSPNRAHRQAGFAVLKAPDVPSVLVELGYLSNR
                     GDEELLATGGGRAKIIDAIVRAVDRHFATRTAESH"
     gene            9601..12048
                     /locus_tag="VGB88_01020"
     CDS             9601..12048
                     /locus_tag="VGB88_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX9646046.1"
                     /translation="MLRALTSLILFAAALAVVAAVAVLWGLHHYGRGLPPYAQLSDYE
                     PPTLTRLHAGDGRLLAEFATEQRVFVPIEAMPDMLVKAFVAAEDQNFYRHPGIDVIGI
                     VRAAVQNLRNLGTTRRLIGASTITQQVAKNFLLTNEVSLERKIKEALLALRVERALSK
                     ERILELYLNEIYLGQRAYGAAAAALNYFDKSLDELTLSEMAFLAGLPKAPNNYQPERN
                     PEAARSRRDYVIGRMREDGYITAAAARQAVAEPLVMHQPDPARSTDAPYFAEEVRREI
                     GRRYGDSALYQGGLSVRTTLDPRLQEIARQALRDGLAAYDRSHGWRGPVARLELGDGW
                     PARLAEVPAPAGLAPWRLAVVREVAAAEAGIGFADGSPGVVPFEHMQWARPWRKDQRI
                     GPEPKAAGDVLAVGDVVAVEAAAKPGADDGAETDPALFALRQIPDVEGALVALDPHTG
                     RVLAMVGGWSFEESEFNRATQAARQPGSAFKPFVYLTALENGYTPADIVLDAPVVIDQ
                     GPGLPKWRPKNYSAEFSGPSTLRRGVEKSRNLMTVRLAHAVGIEKVVAAARRFGVGDF
                     PPVLSMALGAGETTLLRLATGYAMLVNGGRRIEPALIERIQDRHGQTIYRRDARACDG
                     CQDLAAPGDQPPALPDPRERVTDAATAFQITWILKGVVDRGTGARIARLGRPLGGKTG
                     TTNDSNDAWFMGFAPDLVAGVFVGFDAPRTLGPRQTGASVAAPIFQEFMGRALEGQPP
                     VPFRIPPGVRMVRVDADTGLLPTPASEHVLVEAFKLGTEPSSATSASISRPMIGGGGE
                     VIVAPATAPARFDSGLY"
     gene            12104..13226
                     /gene="prfB"
                     /locus_tag="VGB88_01025"
     CDS             join(12104..12172,12174..13226)
                     /gene="prfB"
                     /locus_tag="VGB88_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_142898939.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9646047.1"
                     /translation="MRAELQAAAEDIEQSLALLRRHLDPDASRQRLTDLNARAEDPAL
                     WHDPERAQALMRERNRLERALGDYDAIVKEFTDTCELVELAEADGDEALLEETAQSLA
                     SLHRRAERIEFETLLSGEADDNDCFVEIHAGAGGTESQDWAEMLLRMYSRWADSHGYK
                     VEFIEESPGEEAGLKSVTFKVVGENAYGWLRTESGVHRLVRISPFDSSARRHTSFASV
                     WVYPVVDEAIEIEINDKDLRIDTYRSSGAGGQHVNKTDSAVRMTHLPTGIVVQCQNER
                     SQHKNRAAAMSMLRSRLYELELQRRRDEQQAQEAMKADIGWGHQIRSYVLQPYQMVKD
                     LRTDVQTSNTAAVLDGDIDRFISAALAAQVGNQRAAKGA"
     gene            13240..14280
                     /locus_tag="VGB88_01030"
     CDS             13240..14280
                     /locus_tag="VGB88_01030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9646048.1"
                     /translation="MTGDAKQDANGKLFQAVLGSEQVWIVDIGLKAGLLAAIDAAGAG
                     GVGADELAAELGFDRHYTAVWLRAAYAFELLDCDDGRRFRLAAHMRELLLAEDDPMFI
                     GGRMQLVAALYEDFRAYPSHLRSGATWLRSYHDPWLLEALKNTTKPDPVMITRHVLPQ
                     CAGAIARLEEGGSIVDIGAGGGFAVTHYAERFPAAQVIGLEFDAPSVELMRRTIAAAG
                     LDARIDVRQADANRIEEVDAWDVVTLNITLHETGAEPEWRNVLARAHRALKRGGAIVV
                     SELPYPDALAAYRASPVYRALAGVQIHEAIVGCGMITQSQLGALLRDAGFAAARVADQ
                     PMATRFVMIAEK"
     gene            complement(14329..15246)
                     /locus_tag="VGB88_01035"
     CDS             complement(14329..15246)
                     /locus_tag="VGB88_01035"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HEX9646049.1"
                     /translation="MTGRITGLACAACALAVATGSVQAMPLQMRDTGPAMGARQSNTY
                     HGPGVGALRGGQLERGGQAGASESTTAAVPAAAPAWMVEQEVMSVRDGGTEFVVRIEN
                     VSPADAMRYSDGSSDGAAFSHVAYAVHDAGWPIFEPGEATLPASGLEQLAEDGNVRPI
                     QANLEQHADVVESGIVYRPVGDDKDNELWPGHVYEFHLTADAGENLSFAAMLIQSNDV
                     VVGPKDGYLPLFDSNGRPMSGDVTDRIAFFDAGTEVNEDPKFGPNVGVNQKWLNAGET
                     ETKPVRAPDDGFAWPAPAKVLKVTIQPVG"
     gene            15453..16235
                     /locus_tag="VGB88_01040"
     CDS             15453..16235
                     /locus_tag="VGB88_01040"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9646050.1"
                     /translation="MSAAAATATKGLLTPGELDGFRRDGFLVQRRLLAGRIGEISRWV
                     DELAALPEAAGRHWVYYEDSVTDPGQRVLSRIEYFTRFHDRLSALMHGPELRGRAGEL
                     LGEPALLFKEKVNFKMPGGQGFAPHQDVQAGWDDYGALHISAMLTVDRATEENGCLEL
                     AAGHHKRGLIGAKWKPLEGAELDGIEFVKVPTEPGDVVFFDSFTPHRSAPNLSPHRRR
                     ILYITYGRASDGDQRERYFADKHKSFPPDIERDPDRTYEYKV"
BASE COUNT         2231 a         5923 c         5695 g         2405 t
ORIGIN      
        1 gggtcgccgc cgcctgaggc gcggccggcg gctcagctcg acactcagtt cgacaggccg
       61 gccgcgaccg tcgcgcgcga ctccagaagc tcgaccgcgc aggccaggat cgggtcctcg
      121 cgctggcccg aggtcgggca cgcctggtcg gcggtcagct tggcggtccc ggtaacgtcg
      181 gccgaatcgc tcggcagcgg gttctcgagg tcggcctcgc gccgcatccc ggcctcgtcg
      241 accccgacca cgatgtccgg cacgatcccg gacagctgga tcgagcgccc ggacggcgtg
      301 aagtagcgcg ccgtcgtcag ccgcaccgcg ccgccgcccg acaccgggat gatggtctgc
      361 accgagccct tgccgaacga gcgcgtgccg atcaccaccg cgcgctggtg gtcctggagc
      421 gagcccgaga cgatctccga ggccgaggcc gagccgccgt tgatcagcac cacgatcggc
      481 aggccgtcgg cgatgtcgcc gccctcggcc gagaaccggc gcaccgtgtt ccgctcgcgc
      541 ccgcgcgtcg acacgatctc gccgcccgac aggaaggcat ccgagacgcc gagcgcctgg
      601 tcgagcagcc cgcccgggtt gttgcgcagg tcgatcacca gtccggccat ccgcttgccg
      661 agctccccgc gcaggtcctt gaccgcacgg cgcagcgctt cctcggcctg ctcgttgaac
      721 gtggtgatgc ggacatagcc gatgtcgcct tcggcgtgcg cgcgcaccgc ctggatgcgg
      781 acggtgtcgc gcacgatcgc cacccggaag gtctccgtcg cgggcggccg gcgcagctcc
      841 agcaccaccc gcgagccggc cgggccgcgc atcaggttca ccgcctgctc caaggacatg
      901 ccgaacaccg aggtgccgtc gacatgggtg atcaggtcgc cggcctgaag gccggcccgc
      961 tggcccgggg actcgtcgat cggcgccacc accttgacca ggccgtcctc gaccgtgacc
     1021 tcgatgccga tgccgccgaa ctcgccgcgc gtcctcagct tcatctcgcg gtagcgttcc
     1081 ggcgacaggt agcccgagtg cgaatcgagc ttggacagca tgcggtcgag gccggccgac
     1141 atcaggttgt cggcggtgag ctcgtcgtcc tcgtcctcgg cgtcagttgc cggcgcgtcc
     1201 gcgcgctcgc gcaggtccgc ctcgatcccg acctggtcga agccctcgac cgcgaggccg
     1261 atcatgtgcg gcatcgccac cggctcgaca tagtcgccgc gggtgatgtc gagcacctcg
     1321 gcaaacaggt cgagctggcg ctggcgcagg tcggcgtcgg ggcgcggggc gacgttgcga
     1381 tcgaacgccg ccgacacctc ggcgagctcc gggtaatcct tggccgtcga gtcgaagctg
     1441 gcatggctga agccgagcgg ccgtgagctg agcccgtgcg agttgcagct cgccgcgatc
     1501 aggaccaccg cgaccgcggc ccacagcccg acgctgccgc gcagcagcgg ccgtgcgccg
     1561 cccggagcgg gtgtgcgcgg agccatcagc cggaccgacg ctcggcgatc gcacgctcca
     1621 ggacctcggc gtcgaccgcg cccgggaaca tgcggtcctc gacgatgaag gtgggcgtgc
     1681 cgttgatgtc gagctcgcgc gcgagcgcgt ggttggcatc gatctgcgcc gcgatcgctg
     1741 cggactccat gtcctgcgcc aggcgctcga cgtcgaggcc cacctcggct gcgatgtcca
     1801 tgatcgcccg gtcgctgagg gtgccgcgcg aggacatcag cgcataatgg aactgcaggt
     1861 acttgccctg gctgaccgac gccagcgccg ccctggaggc gcgctcggac tcgggcgtca
     1921 ggatcgggaa ctccttgaac accatgcgga tgttcggatc gctcttgatc agcgccatga
     1981 ccgtgtcgag cgaccgcttg cagtagccgc aacgataatc gaagaactcg accacggtga
     2041 cgtcgccgtc ggggttgccg gcgaccggcg ttgccgggtc gtaccggatc tggtcgccga
     2101 gcgcgatcag gttggtctgg gcgcgctcgc gcgcggacgt ctcctcgcga tcgcgaaggg
     2161 cgcgcacgga atcgaggatc acctcggggt gctcgtggag atagcgctcg atcagcgctt
     2221 cgacctcggc ctgctgggcg tcgctcagcg cctcgtcggc gcgcgccggc ggcaccgccg
     2281 tcgcccaccc cagcgccagc gccgcgacca ccacggcgat cgctgccgcc gcacgcgccg
     2341 cacggccgcc gcccatcacg cctctcgccg attccagcat ggtcaactca caacaattcg
     2401 atttcgagac aatgcctttg cgcttgaggc tcgggccctg gacggggcat cgcgccatcc
     2461 taattaggat ggtcgcaaac gaaaccgcga acaacctttt tccctttccg ccgccgaggt
     2521 gcacaggaat gtgagcgcgg ccgcgcgatc gcgtcgcgct caccgctcgc gccgctcctg
     2581 cagcgccttg agctgctcgg cctcggtctt gatgtcctgg gccctgagcc agcccggcgt
     2641 gccctcggcg tattcgcgct cggcgcgccc ggcgtgcacg ctcgcgttct ccacctggcc
     2701 gatcagcagc gcgtactcgc ccgcggcgag cgacgaggcg ccgacgtcgc cgacctggtg
     2761 ataggcggtg gcgagcaggc gccaggtcga cgggttgtcg tggtcccggc ggttggcgcg
     2821 ttccaggttc tcgatcgccg gcgccaccag cgacgcgtcc ccggtctcga cctgcgcctg
     2881 gccaaggccg gtcagcagca agggatcgtc gggcctcatg ttcaccgcct gctggtaata
     2941 cgtgaccgcc tcgcgcagct tggcgtgctc gaacatgatc tggcccttga gctcgacgaa
     3001 gtacgggtcg tcgggccgct cggcgatcag gctgtcgatc tcggccaacg ccttgtcgcg
     3061 gtcgggcagg cgcaggtacg ccaccgcgcg cgcgtagcgc gccgccaggc tggtgtcggt
     3121 ctcggggtag aagctcttca tcacccgctg cgggcgctcg aggtagccga tcagctttgc
     3181 cttcatgcgc gcgtgccgtt ccatctccgc ctcggatgcg gcctgcgacg tccagcgcga
     3241 ggcgcgcaca tgctcggcga tatgctcgat gcggtcgcgg ctcagcgggt gggtgcgcac
     3301 ataggggtcc tggcgcgagg tgaccagcag ctcctggtcc tggagccgct ccaggaagct
     3361 gagcagaccc gccgccgact gcttggtgcg gtcgaggtag ttgagcgcag cctggtcggc
     3421 ggcgctttcc tgggcgcggg tgtaggcgag gaaggtgcgc aggccgaccg actggccgcc
     3481 ggcgatcacg gcgccgccgg cgcgcgcggt cgcggcaccg gacgcggcgc cgaccgcgat
     3541 caggccgatg ccgagcaagg tggcgaacag gccgaggttc tgggcctcgc ggatggcatc
     3601 gccggcgcgc gccagatggc cgccggcgat gtggccggtc tcgtgcgcga tcaccccgat
     3661 cagctgcccc ggaaagtcgg tggcggtcag cagcccggtg tggatgaaca ggttctggcc
     3721 gccggcgacg aacgcgttca gcgcctcgtc gttgaccagc aggatgcgga tatcggccgg
     3781 gttcaggccc gccgccacga acagtggatc ggcccagccg cggatggtat cttcgatctc
     3841 ggaatcgcgg atcaggggca gattctgggc ccacgccgtg gtcgccgcga cctgcgctgc
     3901 cgccacgcat gcggcgagaa gaagtctgaa caggcgagag cccaccggcg ttccttccga
     3961 gttaccattt caagctaggt accggcatcg atcaggtcaa tggacgcccg cgcgcctgca
     4021 tcgagccgtg gacccgggcc gggtgatgac gctcaaggca tctgagcgca gcgcgatcgc
     4081 gccgttctac gcgatggagg tgctgcgcgc cgccaatcgg cgggcggcgg agggggcgca
     4141 cgtgctgcac ctcgaggtcg gcgagcccgg cgccggcgcg cccgagccgg tgctcgccgc
     4201 ggcgcgggcg gcgatcggcc gggtcaggct aggctacacc gaggcgctgg gcacgccggc
     4261 gctgcgcgcg cgcatcgcgc gccattaccg ggaccgctac gccctcgccg tcgatccggc
     4321 gcgcgtcgcg gtgaccatcg gcgcctcggg ggcgttcctg ctcgccttcc tagccgcctt
     4381 cgacgtcggc gaccgggtgg cggtcgccga gccctgttat ccggcctatc gcaacatcct
     4441 gagcgcgctc ggcatcgagg tggtggcgtt gcaggccggc cccgagcacc ggttccagcc
     4501 gaccgtcgag ctgctcgacc aggcgggccc gctcgacggc ctggtcgtgg ccagcccgtc
     4561 gaacccgacc gggaccatgc tcgcgcccga ccagctggcg cgcctggcgg ggtggtgcga
     4621 ggcgcacggc gtgcgcctga tctcggacga gatctatcat ggcatcacct acggcatggc
     4681 cgagagctgc gcgctcgccc acagcgagcg tgcgctggtg gtcaacagct tctccaagta
     4741 cttcgccatg accggctggc gcctgggctg gatggtgatg agcgaggacg tcgccggcgc
     4801 agtcgatcgc ctggcccaga acctgttcgt ctcgcccgct gccctgccgc agcacgcggc
     4861 gctggtggcg ttcgactgcc gcgaccagct cgatgcctac gtcgcgggct acgcgcgcaa
     4921 ccgcaagatg ctgctcgagc gcctgcccga ggccgggttc gaccgcctgg cgcccgccga
     4981 cggcgccttc tacctgtatg ccgacgtctc gcgcctggcc aacgactcgc aagagctgtg
     5041 cgcgcggatg ctcgacgaga tcggcgtcgc cgccaccccg ggggtcgact tcgacccccg
     5101 gcgcggccac cgctatgtgc ggttctcgtt cgccggcggc gaggccgaga tcgccgccgc
     5161 cgccgagcgc atcgtcgcct ggcgcccgta gcgcccgggc cggcccgaaa cgcgccggct
     5221 cagactccgg tcaggcgctg ccaccagccg cgccggcgcg cggtgtcgtc gtcgtcgggc
     5281 tcggccttgc cctcggcgcc ggattcgccg ccgcccgcag ccgggccggc gaaggcgacc
     5341 gcctgctggg ccggtctcac ctcagcgcgg tggccgccgt tcgccccgtt gcccgtgggc
     5401 gcgcggccgc cgtcagccgc ctcgggcgcg gatgccgcga ccggctcgac ggcctggaga
     5461 gccgcggctg ccaccggcgc ggattcaccg gcttcgccat cgcccgtccg ggccgtcttg
     5521 cgggtgcgcc ggcgccgctt gggcttgacg atcgacgcct cggcgtccgc ggctgcgacc
     5581 ggctcgacgg cgccttcggc ttcgcccgca ggctcggcgg ctcccggctc gccgccgtcg
     5641 gcggtggcgt gctgcggctc tgccgccgcc tccgtctcgc gagtgcgcga gcggcggcgg
     5701 ccgccacgcc ggccgcgacg gcggcggcgc gggcgcgcct cgccctcgcc ggcctcggcc
     5761 tcggcctcgg cctcgccggc aaattcggcg gtctcggctt cggcttcctc ctcggcggcc
     5821 tctgcctcat cggcctccgc cccggccggc ctcgccggct cttcggcgcg ccgccgccgg
     5881 cgccgccgcc ccggttcctc cgcgaccttc tcggccgtct ccgccgtcat ctcggccgcc
     5941 accgcctcgg ccaagaccgc gggcaccacg accggctcgg tgcgcggcgt gaccagctcg
     6001 atccggcagg tcggcggcgc cgggctgtcg tcggtggcga tcctgaccga gatgccgtag
     6061 cggctttcga tctcggccag tgccgcgcgc ttgttgttga gcagatacag cgcgaccggc
     6121 gagggcatgg tcgcctggat cccggccgcc cggccgcgga tgccctcgtc ctcgatcacc
     6181 ctcagcgcgt gcagcgcgat cgactcgacc gagcgcacgc ggccggtgcc ggcgcagtgc
     6241 tggcagttga ctgagctcgc ctcgagcagg ctcgggcgca ggcgctggcg cgacagctcg
     6301 agcaggccga agctcgagat gcggcccatc tggatgcgcg cgcgatcgga ctgcaccgct
     6361 tccttcaggc gcttctcgac cgcgcgcacg ttgcggtgct cctccatgtc gatgaaatcg
     6421 atcacgatca ggccggcaag gtcgcgcagg cgcagctggc gcgcgatctc gtcggcggcc
     6481 tcgaggttgg tcgcggtcgc ggtctcctcg atgttgcgct cgcgcgtcga gcggcccgag
     6541 ttgacgtcga tggcgaccag cgcctcggtc ggatcgaaca ccacatagcc gcccgacggc
     6601 agctcgacgg tagcgttgtg catggtgtcg agctggctgt cgacctggta gcgggtgaac
     6661 agcggcagcc cgtccttgta cggctggacc cgcttggcgt ggctcggcat cagggtacgc
     6721 atgaagtcct tggcgacccg atagccctca tcgccctcga ccagcacctc gaccatcttc
     6781 gaatcgtaca ggtcgcggat cgagcgcctg atcaggttgg cctcctcgtg gatcagcgcc
     6841 ggggcggtcg ccttcagcgt gctctcgcga atctcgtccc acaggcgcat gaggtactcg
     6901 aagtcgcgcc ggatctcggc cttggtgcgt tcctggccgg cggtgcgcag gatcaccgcc
     6961 atgccctcgg acaacgacag cgaggcgacc accgagcgca ggcgcttgcg cgcctcgccg
     7021 gactggatct tgcggctgat gccgccgccg cgcgtggtat tgggcatcag cacgcagtag
     7081 cgcccggcaa gcgagatgta ggtggtgagc gcggcgccct tggtgccccg ctcctccttg
     7141 gtcacctgga ccagcaggat ctgccggcgc cggatcacct cctggatgcg gtagcgcggc
     7201 aaggtcgccg gccgcctggc gatctcctcc tgttcctggc cgctgaccgt ctccaccggc
     7261 tcggcgtcgt cgtcctcggc cgccgccggc tcgcggggcg cgccccgctc gtcgtcgtcc
     7321 gccgccgcct ccttgcgctg ctgggcgatc agcgccttac ggtcggagac cgggatctga
     7381 tagtagtcgg ggtggatctc gttgaaggcg aggaagccgt gccggttgcc gccgtattcg
     7441 acgaacgccg cctgcagcga cggttcgacg cgggtaacct tggccagata aacgttgccc
     7501 ttcagctgcc tcttggtact ggactcgaaa tcgaactctt cgagcttctt tccgtcgacc
     7561 accaccacgc gggtctcttc cgtgtgggtg gcgtcgatga gcatgcgttt tgccatctag
     7621 tgtctttcct tgtcggctgc ggcgctgcgg cggatagccg cgccacgcgc gaacgctgcg
     7681 cgccggtcga cgttgggggt gctgccgcga ccctggcgcg cacgaatcgc cggcggcggg
     7741 cgtcgccgcc cgggcgcacg acgtgcccgc gggacggcgt cgcccgactg ccctgatcag
     7801 ggcttgccgg ggtgcgacca aaggtgggga ctgccatcat tcagagaact cctggggccg
     7861 cggaatgccg tttggcggct cttgagcaga ggacgccggt ttcgcggacc aatgccaggc
     7921 gggcgaatct acggttcgaa tccgggacgg cgaccgccgc cccaaccgcc accacgagcc
     7981 cggcgaccaa tcgcgcaacg cgacgggcgg ccggcccgcg gctcggcagg cccgtggatc
     8041 atacggcacc gccggcgcat cgacaatgcc cgaatgcgcc gccgccacgg ccggcggcaa
     8101 tcggttgtta atcctcgcgc gccaggattg cctgggccgt tgccaaggct actataatgg
     8161 aatgcgcgtg ctgcccgttg acgccctgac ggacaacgcc gacgcccgcc cgtcggaaac
     8221 ctcgaccgcg gtttccggag ggcggcatgc gcgctttgcg ctcgcctgcg ccgtcggctt
     8281 ggtggtggcc tcaggcctcg ccggcgcggc acacgccgtg cccgaggcca gcgaggcgcg
     8341 ggcgtggcgg catccggact acacccgcct ggtgatcgag ctgagcgagc aggtcgagtt
     8401 cagcatcttc accctggccg agccgaggcg catggtggtc gacttccccg agctcgactt
     8461 ccgcctgccg ccgaggccgt tcggcgacgg cgcggtcggc gtgatcgcga ccatgcgcta
     8521 cggcctgttc gagcccggcg tctcgcgcct ggtgctcgac ctcgcccgcc cggcgcgggt
     8581 gcgcgactcg ttcctgatgc ctcccagcga gggcaagccg taccgcttcg tgctcgacct
     8641 cgaggagacc gacgccgccg acttcgccgc cgccgcccag gccaatccgc cgccgaggcc
     8701 cgtcccgggg ccggtgatcg agccgcccgc ggccaccgtg gcgccgcgca gcgacggcaa
     8761 gcgggtgatc gtggtcgatg ccggccacgg cggcgtcgat ccgggcgcga tcagcgcctc
     8821 gggcctgtac gagaagcaga tcgcgctcgt cgccgcgcgc gagctcgccg agcggctgca
     8881 ggcgaccggc cgctaccagg tggtcatgac ccgcgaccgc gacatcttcc tgtccttgcg
     8941 ctcccgggtc tcggtggcgc gccgcgccgg cgccgacctg ttcgtgtcgc tgcacgccga
     9001 ctcgatcgcc gacaagggag tgcgcggcag ccacgtctac tcgctgtcca agaccgcctc
     9061 cgacgccgcg gcggcggcgc tggcggcggc cgagaacaag tccgacgtga tcgccggcct
     9121 cgacctcgcc gaatacacgc ccgaggtcca taccatcctg ctcgacctca gccagcgcga
     9181 gaccaacaac acctccgccg aggtggcgag ccgcctggtc gacgaattgc agcgcaacgg
     9241 catccgctcg cccaatcgcg cgcaccggca ggccgggttc gcggtgctca aagcgcccga
     9301 cgtgccgtcg gtgctggtcg agctcggcta cctgtccaac cgcggcgacg aggagctgct
     9361 ggcgaccggt ggcggccggg cgaagatcat cgacgcgatc gtgcgcgccg tcgaccgtca
     9421 tttcgccacc cgcacggccg agtcgcattg accgacggcg gcgcgccggg ggcggcgcca
     9481 catcgtcgcc acattgctcg cctaaattga tatcttgcca atgcttcctc gacgaacgca
     9541 cgggccgccg atgtcgccga cgaaggcggg ctttcgtgcc ctgaggccat tcggttgccg
     9601 atgctgcgcg cactcacctc actgatcctg ttcgccgcgg cgctggcggt cgtggcggcg
     9661 gtcgcggtgc tttgggggct gcaccattac ggccgcggcc tgccgcccta tgcccagctc
     9721 tccgactacg agccgccgac cctgacccgg ctgcacgccg gcgacggccg cctgctcgcc
     9781 gagttcgcca ccgagcagcg cgtgttcgtg ccgatcgagg cgatgcccga catgctggtc
     9841 aaggcgttcg tcgccgccga ggaccagaac ttctatcgcc atcccggcat cgacgtgatc
     9901 ggcatcgtgc gcgccgcggt ccagaacctg cgcaatctcg gcaccacccg ccgcctgatc
     9961 ggcgcctcca ccatcaccca gcaggtggcc aagaacttcc tgctcaccaa cgaggtctcg
    10021 ctcgagcgca agatcaagga ggcgctgctc gcgctacgcg tcgagcgggc gctgagcaag
    10081 gagcggatcc tcgagctgta cctcaacgag atctatctcg gccagcgcgc ctatggcgcc
    10141 gccgccgcgg cgctcaacta tttcgacaag tcgctcgacg agctgaccct gtccgagatg
    10201 gcgttcctcg ccggcctgcc caaggcgccc aacaactacc agccggagcg caatccggag
    10261 gccgcgcgca gccggcgcga ctacgtcatc ggccggatgc gcgaggacgg ctacatcacc
    10321 gccgccgcgg cccgccaggc ggtggccgag ccgctggtca tgcaccagcc cgatcccgcg
    10381 cgcagcaccg acgcgcccta tttcgccgag gaggtgcggc gcgagatcgg ccggcgctac
    10441 ggcgacagcg ccctctacca gggcgggttg tcggtgcgca ccacgctcga tccgcgcctg
    10501 caggagatcg cccgccaggc gctgcgcgac ggcctggccg cctatgatcg cagccacggc
    10561 tggcgtgggc cggtggcgcg cctggagctc ggcgacggct ggccggcgcg cctcgccgag
    10621 gtgccggcgc cggcgggcct cgcgccgtgg cggctggccg tggtgcgcga ggtcgccgcc
    10681 gccgaggccg gcatcggctt cgccgacggc agcccgggcg tggtgccgtt cgagcacatg
    10741 cagtgggcgc gcccctggcg caaggaccag cgcatcgggc ccgagcccaa ggccgcgggc
    10801 gacgtgctgg cggtcggcga cgtggtcgcg gtcgaggccg cggccaagcc cggcgccgac
    10861 gacggggccg agaccgatcc ggcgctgttc gcgctgcgcc agattcccga cgtcgagggc
    10921 gcgctcgtcg cgctcgaccc ccacaccggg cgggtgctgg cgatggtcgg cggctggagc
    10981 ttcgaggaaa gcgagttcaa ccgcgccacc caggccgccc gccagccggg ctcagcgttc
    11041 aagccgttcg tctacctgac cgcgctcgag aacggctaca ccccggccga catcgtgctc
    11101 gatgcgccgg tggtgatcga ccagggcccg ggcctgccca agtggcggcc gaagaactac
    11161 tcggccgagt tctcgggccc gtcgaccttg cgccgcgggg tcgagaagtc gcgcaacctg
    11221 atgaccgtgc gcctggcgca cgcggtcggc atcgagaagg tggtcgccgc cgcgcgccgg
    11281 ttcggtgtcg gcgactttcc gccggtgctg tcgatggcgc tcggcgccgg cgagacgacg
    11341 ttgctcaggc tggccaccgg ctacgccatg ctggtcaacg gcggccggcg catcgagccg
    11401 gccttgatcg agcgcatcca ggaccgccac ggccagacca tctatcggcg cgacgcgcgc
    11461 gcctgcgacg gctgccagga tctcgccgcc cccggcgacc agccgccggc gctgccggat
    11521 ccgcgcgagc gcgtgaccga tgccgcgacc gcgttccaga tcacctggat cctgaagggc
    11581 gtggtcgatc gcggcaccgg cgcgcgcatc gcccgcctcg gccggccgct gggcggcaag
    11641 accgggacca ccaacgacag caacgacgcc tggttcatgg gcttcgcgcc ggacctggtg
    11701 gccggggtgt tcgtcggctt cgacgccccg cgcacgctcg ggccgcgcca gaccggcgcc
    11761 agcgtcgcgg cgccgatctt ccaggagttc atgggccgcg cgctcgaggg ccagccgccg
    11821 gtgccgttcc gcatcccccc gggcgtgcgc atggtgcgcg tcgacgccga caccgggctg
    11881 ttgcccacgc ccgccagcga gcacgtgctg gtcgaggcat tcaagctcgg caccgagccg
    11941 agctccgcca cctcggccag catcagccgg ccgatgatcg gcggcggcgg cgaggtgatc
    12001 gtggcgccgg cgaccgcgcc ggccaggttc gacagcggcc tgtactgacg ctatatgtcg
    12061 ctcggcccgt gcgggcctcg cgtgacccga cccaggagac gccatgcgcg ccgagctgca
    12121 agcggccgcc gaggacatcg agcagtcctt ggccctgctg aggaggcatc tttgaccccg
    12181 atgcctcgcg tcagcgcctc actgacctga atgcgcgcgc cgaggacccg gcgctgtggc
    12241 acgatcctga gcgcgcccag gcgctgatgc gcgagcgcaa tcggctggag cgcgcgctcg
    12301 gcgactacga cgccatcgtc aaggagttca cggacacctg cgagctggtc gagctggccg
    12361 aggccgacgg cgacgaggca ctgctcgagg agacggccca gtcgctcgcc tcgctgcacc
    12421 ggcgcgccga gcgcatcgag ttcgagaccc tgctgtcggg cgaggccgac gacaacgact
    12481 gcttcgtcga gatccatgcc ggcgccggcg gcaccgaaag ccaggactgg gccgagatgc
    12541 tgctgcgcat gtacagccgc tgggccgaca gccacggcta caaggtcgag ttcatcgagg
    12601 agagcccggg cgaggaagcg ggcctgaaga gcgtcacctt caaggtggtg ggcgagaacg
    12661 cctatggctg gctgcgcacc gaaagcgggg tccatcggct ggtgcgcatc tcgccgttcg
    12721 attcctcggc ccgccggcac accagcttcg ccagcgtctg ggtttatccg gtggtcgacg
    12781 aggcgatcga gatcgagatc aacgacaagg acctgcgaat cgatacctac cgctcgtccg
    12841 gcgccggcgg ccagcacgtc aacaagaccg actcggcggt gcgcatgacc cacctgccca
    12901 ccggcatcgt ggtccagtgc cagaacgagc gctcgcagca caagaaccgg gcggcggcga
    12961 tgagcatgct gcgctcgcgc ctgtacgagc tcgagctgca gcgccggcgc gacgagcagc
    13021 aggcccagga ggccatgaag gcggacatcg gctggggcca ccagatccgc tcctacgtgc
    13081 tgcagcccta ccagatggtc aaggacctca ggaccgacgt ccagaccagc aacaccgccg
    13141 cggtgctcga cggcgacatc gaccgcttca tcagcgccgc gctcgccgcc caggtcggca
    13201 accagcgcgc ggccaagggc gcctgagggt gcggcggcga tgaccggcga cgccaagcag
    13261 gacgccaacg gcaagctgtt ccaggcggtg ctcggcagcg agcaggtgtg gatcgtcgac
    13321 atcgggctga aggccggcct gctggcggca atcgacgccg ccggcgcggg cggcgtcggc
    13381 gccgacgagc ttgccgccga gctcggcttc gaccggcact acaccgcggt gtggctgcgc
    13441 gcggcctacg ccttcgagct gctcgactgc gacgacggcc gccgcttccg cctggcggcg
    13501 cacatgcgcg agctgctgct ggccgaggac gacccgatgt tcatcggcgg gcgcatgcag
    13561 ctggtggcgg cgctgtacga ggacttcagg gcgtatccca gccatctcag gagcggcgcg
    13621 acctggctgc gcagctacca cgacccgtgg ctgctggagg cgctcaagaa caccaccaag
    13681 cccgacccgg tgatgatcac ccgccacgtg ctgccgcaat gcgcgggcgc catcgccagg
    13741 ctcgaggagg gcggctcgat cgtcgacatc ggcgccggcg gcggcttcgc ggtcacccac
    13801 tacgccgagc gcttcccggc ggcgcaggtg atcgggctcg agttcgacgc cccgagcgtc
    13861 gagctgatgc gccggaccat cgccgccgcc ggcctcgacg cgcgcatcga cgtgcgccag
    13921 gcggacgcca accggatcga ggaggtcgac gcctgggacg tggtgacctt gaacatcacc
    13981 ctgcacgaga ccggcgccga gcccgagtgg cgcaacgtgc tggcgcgcgc ccaccgggcg
    14041 ctcaagcgcg gcggggcgat cgtggtctcg gagctgccct atcccgacgc gctcgccgcc
    14101 taccgcgcct cgccggtcta ccgggcgctc gccggcgtcc agatccacga ggcgatcgtc
    14161 ggctgcggca tgatcaccca gagccagctc ggcgccctgc tgcgcgacgc cggcttcgcc
    14221 gcggcgcgcg tcgccgacca gccgatggcc acgcgcttcg tgatgatcgc ggagaaatga
    14281 gcccggcgcg gcgggggcag gccaggccgc ccccgccggc ccggaccgtc agccgaccgg
    14341 ctggatggtg accttcagca ccttggccgg cgccggccaa gcgaagccgt cgtccggcgc
    14401 gcgcaccggc ttggtctcgg tctcgccggc gttaagccac ttctggttga ccccgacatt
    14461 ggggccgaac ttgggatcct cgttgacctc ggtgccggcg tcgaagaacg cgatgcggtc
    14521 ggtcacgtcg ccgctcatcg gccggccgtt cgagtcgaac agcggcagat agccgtcctt
    14581 cggcccgacg accacgtcgt tcgactggat cagcatggcc gcgaacgaca agttctcgcc
    14641 ggcgtcggcg gtgaggtgga actcgtagac atgccccggc cacagctcgt tgtccttgtc
    14701 gtcgccgacc ggccggtaga cgattcccga ctcgaccacg tcggcgtgct gctccaggtt
    14761 ggcctggatc gggcggacgt tgccgtcctc ggccagctgc tcgaggccgc ttgccggcag
    14821 ggtcgcctcg ccgggctcga agatcggcca gccggcgtcg tggacggcgt aggcgacgtg
    14881 gctgaacgcg gcgccgtcgc tgctgccgtc ggagtatctc atcgcgtcgg cgggcgagac
    14941 gttctcgatc cgcaccacga actcggtgcc gccgtcgcgc acgctcatca cctcctgctc
    15001 gaccatccac gccggtgccg cggcgggcac cgccgcggtc gtgctttccg aggccccggc
    15061 ctgcccgccg cgctcgagct ggccgccgcg cagggcgccc acgccgggcc cgtgataggt
    15121 gttggattgc ctggccccca tcgccggccc ggtatcgcgc atctgcagcg gcatcgcctg
    15181 gacgctgccg gtcgccaccg ccagcgcgca ggcggcgcaa gcgagaccgg tgattctgcc
    15241 tgtcatgatt tgaacctcca tcgcgtgaca gcttccgccc gtggtgcggc ggatgttggg
    15301 cacagaattg ttcgccgcgc gcgtcagcgc aagtaaccga gtcaaatttc cttgtgatcg
    15361 ggcgtgatcg ggcccggtcg ttgggccttg cccggcgggc gcgcttcggt taggtgtctc
    15421 gcccaacacc aacgccggcg ggatgggacg cgatgtcagc agcagccgca accgcgacca
    15481 aaggattgct tacgccgggc gagctcgacg gcttccggcg cgacgggttc ctggtccagc
    15541 gccggctgct cgccgggcgc atcggcgaaa tctcgcgctg ggttgacgag ctcgcggcgc
    15601 tgcccgaggc ggccggccgg cactgggtgt attacgagga cagcgtcacc gatcccggcc
    15661 agcgggtgct gtcgcggatc gagtatttca cccgctttca cgaccgcttg agcgcgctga
    15721 tgcacggccc cgagctgcgc gggcgggcgg gcgagctgct cggcgagccg gcgctgctgt
    15781 tcaaggagaa ggtcaacttc aagatgcccg gcggccaggg cttcgcgccg catcaggacg
    15841 tgcaggcggg ctgggacgac tatggcgcgc tgcatatctc ggcgatgctc accgtcgatc
    15901 gcgcgaccga ggagaatggc tgcctggagc tcgccgccgg ccaccacaag cgcggcctga
    15961 tcggcgccaa atggaagccg ctggagggcg cggagctcga cggcatcgag ttcgtcaagg
    16021 tcccgaccga gcccggcgac gtggtgttct tcgattcctt caccccccac cgctcggcgc
    16081 cgaacctgag cccgcatcgc cggcgcatcc tctacatcac ctatggccgg gcgtccgacg
    16141 gcgatcagcg cgagcgctac ttcgccgaca agcacaagag cttcccgccc gacatcgagc
    16201 gcgacccgga ccgcacctac gagtacaagg tctgacgggg cgtccgagca tgcc
//
LOCUS       DASWHU010000031         6038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2227963, whole genome shotgun sequence.
ACCESSION   DASWHU010000031 DASWHU010000000
VERSION     DASWHU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227963"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGB88_01045"
     CDS             <1..213
                     /locus_tag="VGB88_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEX9646051.1"
                     /translation="APVLESYARARSFDTVSMSLVTDGLNRLFSNDSAPLRLARDLGL
                     AAVNRAPPLKRLFMRHAMGLIGTQSS"
     gene            complement(470..1111)
                     /locus_tag="VGB88_01050"
     CDS             complement(470..1111)
                     /locus_tag="VGB88_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9646052.1"
                     /translation="MTETILFGNPASTYVRTVRLALEEKGAKYRIEEIDLKDPAYAAR
                     HPFRRMPSFEHDGVRLFETQAICRYVDEAVAGPALQPGDALGRARMEQWVSAVCDYLY
                     PTAIRGYVLHYVFARRDGREPDRAAIAQSLPTVKERLGIFDAALGKGTYLAGDKLSIA
                     DLFLAPIAFYVCRAMPEKDQMIAGRANLARWLAAMESRPSFAATMPPLAQAAE"
     gene            complement(1136..1606)
                     /locus_tag="VGB88_01055"
     CDS             complement(1136..1606)
                     /locus_tag="VGB88_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9646053.1"
                     /translation="MTGDATVAGDTAHGLVLTVRRMIAAPPARVFAAFTDKDLFAQWF
                     GPHAGKAVVEQMDVRPGGRYRVLLLPDGGETYRLSGSYREVVAGERLAFTWLWDWAAG
                     VETLVSIEFRAVKGGTELVITHSGFPDETLAGEHRKGWLAGLDALAAMLPETRK"
     gene            complement(1596..1931)
                     /locus_tag="VGB88_01060"
     CDS             complement(1596..1931)
                     /locus_tag="VGB88_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9646054.1"
                     /translation="MSATFAALVDPTRRAILQRLAAGETTVGALAKPFDMSLPAVSKH
                     LRVLESAGLIDRRRVGRERHCRLRPAPLRSAADWLDFYRKFWDERLDSLARLFETPPA
                     DAKGQTDDR"
     gene            complement(2009..3082)
                     /locus_tag="VGB88_01065"
     CDS             complement(2009..3082)
                     /locus_tag="VGB88_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9646055.1"
                     /translation="MVGYPKGAGDRAVAPGALTDVVVAIFRACGMGAGDAALLADTMV
                     TADLRGVHSHGCLRVPDYVAKLSGGGADARGRPRVLSARGAAVVVDGGNAMGQIAAAF
                     AIDRAIERAARVHVGVAAVGGSNHCGAMDYYAMRALPHDMIGIATTNALPTMAPWGGR
                     DKILGINPLAIAVPAGAEAPLVIDTAFSASSHGKIRVFRQKGLALPEGWASDAEGRPT
                     TDPSVAADGLLQPIGGYKGTGFALMMGILSTVLSGAAFGTRLGSMATGPKPGRDGHFL
                     MAINIAGFVPPATFKAEVDGIIRELRASRPAPGHERVMLPGEREREAAEAYARDGIPL
                     SAETIVGITAAAARLGVDATALT"
     gene            complement(3111..4169)
                     /locus_tag="VGB88_01070"
     CDS             complement(3111..4169)
                     /locus_tag="VGB88_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9646056.1"
                     /translation="MTTAGAVRKAVATVICAAALAATGGTAGAQEKVTFKLASSYSST
                     LDVVGENIFRVIENIDTMSGGNFKIRFFEPGALVPALEVFDSVATGAVDMSYTTTGFH
                     AGKFPGLVFFSSVPFGPAVNEYLAWIHFGGGKEIYDREYNARGVQGFQCGVVVAESSG
                     WFRDEITSLDQLKGLKMRFFGLGARVMEKFGVSTQLLAGGDIYPALELGTIDATEFSY
                     PRLDRTLGFYQIAKHYYFPGWHQQASLVEVIVNLEKFRALPKAYQKMIEVACGEANMW
                     LMAKGDATQGDALEFLKGHGVQIHKWTDDQIDQFRRAWDEVAEAAAGGDPLFREVYDS
                     YKTFRASYAVWRDLGYLK"
     gene            complement(4275..5285)
                     /locus_tag="VGB88_01075"
     CDS             complement(4275..5285)
                     /locus_tag="VGB88_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646057.1"
                     /translation="MGIELTYGDRQRFRFRTRDVTIPGRVIDARHPVRRAEWRLNGGA
                     PVPFYVEAISDEGVDWVQGYKASPAELRCKEQGDFTIEVPAAASPLNPGDNALDIEVE
                     DAGGQGVRRTLRFTWEPAPVPLPLDLSDLSRIAGIQDVGQVVAGAFDLDRAANLIRSR
                     APVVPDSLLVLGGPHSSQEATYRVRFTSFEGVKWLGPSDFFVGFEDREPPIAIKTGWS
                     SAGMMALNPKGEARCFIAWGDHSNSAREWVVVTRPPAAVALAPKVAYRVRHQVLFADS
                     VDRCRFRIWAEGAPEPDAWLCAESDAGVEPGRTRHRAASFGLFQHSGAAIEWSDIRVR
                     AP"
     gene            5608..>6038
                     /locus_tag="VGB88_01080"
     CDS             5608..>6038
                     /locus_tag="VGB88_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX9646058.1"
                     /translation="MVWLVLGIVLWSLVHAFPSLAPAGRAELVARLGEGPYKGLFALA
                     AVAAVVVMVIGWRAAAPVPVYAPPAGGRELAGIVMLVAFVLFPTARLPSNIRRWVRHP
                     QLTAVALWAAAHLIANGDSRSVLLFGALGLWALIEMVLINRR"
BASE COUNT          858 a         2126 c         2142 g          912 t
ORIGIN      
        1 gccccggtgc tcgagtctta tgcgcgcgcg cgcagcttcg acacggtcag catgagcctg
       61 gtgaccgacg ggctcaaccg cctgttcagc aacgacagcg cgccactccg cctggcccgc
      121 gacctcggcc tcgccgcggt caaccgcgcc ccgccgctca agcgcctgtt catgcgccac
      181 gccatgggtt tgatcggcac tcagtcgagc taacggttgg ccgactaata ccggatcgca
      241 ttgataagga ctcaggccac tgttctcgtt gtcatcgcga gcaaagcgaa gcgatccaga
      301 attctcgttg cgtcgcctgc ggttctggat cgccacgacc gcttcgcggt ctcgcgatga
      361 cgctgccggc gatgggtcaa gatcagtgcg aactggtatt agtatcatcc cgctctagcc
      421 tcgccctgcc tcccccgccg gcgcgggcgt tgccaaggac gtgcaatgcc tactcggcgg
      481 cctgggccag cggcggcatg gtggcggcga agctcggccg cgactccatg gcggcgagcc
      541 agcgggcgag gttcgcccgg cccgcgatca tctggtcctt ctccggcatc gcccggcaga
      601 catagaacgc gatcggcgcc aggaacaggt ccgcgatcga gagcttgtcg ccggcgagat
      661 acgtgccctt cccgagcgcc gcatcgaaga ttccgagccg ctccttgacc gtcggcagcg
      721 actgcgcgat cgccgcccgg tcgggctcgc gcccgtcgcg gcgggcgaac acgtagtgca
      781 gcacatagcc gcggatcgcg gtcggataga ggtagtcgca gaccgcgctc acccactgct
      841 ccatgcgcgc acgcccgagc gcgtcgccgg gctgcagcgc cggcccggcg acggcctcgt
      901 cgacatagcg gcagatcgcc tgggtctcga acaggcgcac gccgtcatgc tcgaaggacg
      961 gcatcctgcg gaacggatgg cgcgcggcat aggccgggtc cttgaggtcg atctcctcga
     1021 tgcggtactt ggcgcccttc tcctcgagcg cgaggcgcac ggtgcgcaca taggtgctgg
     1081 ccggattgcc gaacaggatt gtctcggtca tggtctgggt cctccctcat tcgggttact
     1141 ttcgggtctc gggcagcatg gccgccaggg cgtcgagccc ggcgagccac cccttccggt
     1201 gctcgccggc gagcgtctcg tccgggaagc cggaatgcgt gatcacgagc tcggtgccgc
     1261 ccttgacggc gcggaactcg atgctcacca gggtctcgac cccggccgcc cagtcccaca
     1321 gccaggtgaa cgcgagccgc tcgccggcca cgacctcgcg gtagctgccg gagaggcggt
     1381 acgtttcgcc gccgtcgggc agcagcagca cgcggtagcg gccgcctggg cgcacgtcca
     1441 tctgctcgac caccgccttg ccggcgtggg ggccgaacca ctgggcgaac aggtccttgt
     1501 cggtgaatgc cgcgaacacg cgcgccggcg gcgcggcgat catccggcgg acggtcagca
     1561 ccaggccgtg ggccgtgtcg ccggccaccg tggcgtcacc ggtcatcggt ctgtcctttc
     1621 gcatcggcgg gcggggtctc gaacaggcgg gcgagcgaat cgagccgctc gtcccagaac
     1681 ttgcggtaga agtcgagcca gtcggcggcg ctcctgagcg gcgccggccg caggcggcag
     1741 tgccgttcgc gccccacccg gcggcggtcg atcaggccgg cgctctcgag cacgcgcagg
     1801 tgcttggaga ccgccggcag cgacatgtcg aacggcttgg cgagggcgcc gacggtggtc
     1861 tcgccggccg ccaggcgctg caggatcgcg cggcgcgtcg ggtcgaccag cgcggcgaag
     1921 gtggcgctca tcgcctgctc ggaattgtta accatatggt taagtgttac cgaagacggt
     1981 cagccggtca aacgcgctcc tgtcacgatc acgtgagcgc ggtcgcgtcg actccgaggc
     2041 gggcggcggc ggcggtgatg ccgacgatgg tctcggcgct caaggggatg ccgtcgcggg
     2101 cgtaggcctc ggccgcctcg cgctcgcgct cgcccggcag catcacgcgc tcgtggccgg
     2161 gcgcggggcg gctcgcgcgg agctcgcgga tgatgccgtc gacctcggcc ttgaaggtcg
     2221 cgggcggaac aaagcccgcg atgttgatcg ccatcaggaa gtggccgtcg cgcccgggct
     2281 tgggcccggt cgccatcgag ccgaggcggg tgccgaacgc cgcgcccgac agcaccgtcg
     2341 agagtatccc catcatcagc gcgaagccgg tgcccttgta gccgccgatc ggctgcaaca
     2401 ggccgtcggc ggcgacggag gggtcggtgg tcgggcggcc ctcggcgtcg ctcgcccagc
     2461 cctcgggcag cgccaggccc ttctgccgga acaccctgat cttgccgtgc gaggaggcgc
     2521 tgaacgcggt gtcgatcacc agcggcgcct ccgcgcccgc cggtaccgcg atcgccagcg
     2581 ggttgatgcc gaggatcttg tcgcgcccgc cccacggcgc catggtcggt agcgcgttgg
     2641 tggtggcgat gccgatcatg tcgtggggaa gcgcgcgcat ggcgtaatag tccatcgccc
     2701 cgcaatggtt gctgccgccg accgcggcga ctccgacatg gacccgggcc gcgcgctcga
     2761 tcgcccggtc gatcgcgaag gcggcggcga tctggcccat ggcgttgccg ccgtcgacca
     2821 ccaccgccgc gccccgggca ctgagcacgc gcgggcgccc gcgcgcatcg gcgccgccgc
     2881 cgctgagctt ggcgacgtag tcgggcacgc gcaagcaacc gtggctgtgg acgccgcgca
     2941 ggtccgcggt gaccatggtg tcggcaagca gcgccgcatc gcccgcgccc atgccgcagg
     3001 cgcggaagat ggcgaccacg acgtccgtga gcgcgcccgg ggcgacggcg cggtcgcccg
     3061 cgcccttggg atagcccacc attgcccggc cggcgccggc gcgcgccggg ttacttcagg
     3121 tagccgaggt cgcgccacac ggcgtaggac gcgcggaacg tcttgtagct gtcatagacc
     3181 tcgcggaaca gcgggtcccc gcccgccgcc gcctcggcga cctcgtccca ggcgcggcgg
     3241 aactggtcga tctggtcgtc ggtccatttg tggatctgca ccccgtggcc cttgaggaac
     3301 tcgagcgcgt cgccctgggt ggcgtcgccc ttggccatca gccacatgtt ggcctcgccg
     3361 caggcgacct cgatcatctt ctggtaggcc ttgggcagcg cccggaactt ctccaaattc
     3421 acgatcacct cgaccagcga cgcctgctga tgccagcccg ggaagtagta gtgcttggcg
     3481 atctggtaga agccgagcgt gcggtcgagc cggggatagg agaactcggt ggcgtcgatg
     3541 gtgccgagct cgagcgccgg atagatgtcg ccgccggcca acagctgggt ggagaccccg
     3601 aacttctcca tcacccgcgc gccgaggccg aagaagcgca tcttgagccc cttgagctgg
     3661 tcgagcgagg tgatctcgtc gcggaaccag cccgacgact ccgccaccac cacgccgcac
     3721 tggaagccct ggacgccgcg cgcgttgtac tcgcggtcgt agatctcctt gccgccgccg
     3781 aagtggatcc acgccaggta ctcgttgacc gccgggccga acggcaccga gctgaagaac
     3841 accaggcccg ggaacttgcc ggcgtggaag ccggtggtgg tgtaggacat gtcgaccgcg
     3901 ccggtggcca ccgagtcgaa cacctcgagc gcgggcacca gcgcgcccgg ctcgaagaag
     3961 cggatcttga agttgccgcc cgacatggtg tcgatgttct cgatcacccg aaagatgttc
     4021 tcgccgacca cgtcgagcgt gctcgaatag gacgaggcga gcttgaacgt caccttctcc
     4081 tgggccccgg ccgttccccc cgtcgccgcc agcgcggcgg cgcagatgac ggttgccacg
     4141 gccttgcgga ctgcgcctgc cgtcgtcatg gcgtcctccc ctgctgatgg atgtggttcg
     4201 cgccgggcgc gcccggcggt cccgactcta tcatcggcga cgcgcgtgtg gggcggaaat
     4261 ggcggcgagg cgcgctacgg cgcgcgcacg cggatgtccg accactcgat cgccgcgccc
     4321 gagtgctgga acaggccgaa cgaggcggca cggtggcgcg tgcggcccgg ctcgacgccg
     4381 gcgtcgctct ccgcgcacag ccacgcatcg ggctcgggcg cgccctcggc ccagatgcgg
     4441 aaccggcagc ggtcgacgct gtccgcgaac agcacctggt ggcggacccg gtaggcgacc
     4501 ttgggcgcga gcgcgaccgc cgccggcggc cgggtcacca ccacccattc gcgcgcgctg
     4561 ttcgagtggt cgccccaggc gatgaagcag cgcgcctcgc ccttcgggtt gagcgccatc
     4621 atgccggcgc tcgaccagcc ggtcttgatc gcgatcggcg gctcccggtc ctcgaagccg
     4681 acgaagaagt cgctcgggcc gagccacttg acaccctcga aggaggtgaa gcggacgcgg
     4741 taggtcgcct cctggctcga atgcggcccg cccagcacga gcagcgaatc gggcaccacc
     4801 ggcgcgcgcg agcggatcag gttggcggcg cggtcgaggt cgaaggcgcc ggcgaccacc
     4861 tggcccacgt cctggatgcc agcgatgcgc gagaggtccg aaagatcgag cggcagcggt
     4921 accggcgccg gctcccaggt gaagcgcagc gtgcgccgca cgccctgccc gcccgcgtcc
     4981 tcgacctcga tgtcgagggc gttgtcgccg ggattgagcg ggctcgccgc ggccgggacc
     5041 tcgatcgtga aatcgccctg ctccttgcag cgcagctccg ccggcgaggc cttgtagcct
     5101 tgcacccagt cgacgccttc gtccgagatc gcttcgacgt agaacggcac cggcgcgccc
     5161 ccgttcagcc gccactcggc gcggcgcacc gggtgccgcg catcgatcac gcgcccggga
     5221 atcgtgacgt cgcgggtgcg gaagcggaac cgctggcggt cgccgtatgt cagctcgatg
     5281 cccatcctcg cgcgccattt gcgccgcccc aagtcggcgc gtcaagcgcc ggtccattgg
     5341 catgcgagaa ttgaccgaag ctgcgaaaga gcttcggcca cgcggagacg cggagggcgc
     5401 ggtggcggag tgagaagcac ggcgaccttg ccagccgaga ccgccagcga cggaaattcg
     5461 tcattttcaa tcatgcggtt ttgcatcgtc cgcctcggga cctccgcgcc tccgcgtgag
     5521 tcaaacgacg ggcgatcgca tggggccggg cgtcccggga cgtgctagtc ttggtcgatt
     5581 cggctgcggc gacagcgggg gcgcgtcatg gtctggctgg tactcggcat tgtcctgtgg
     5641 agcctggtcc acgcgttccc gagcctggcg ccggcggggc gggcggagct ggtggcgagg
     5701 ctcggcgagg gcccctacaa gggcctgttc gcgctggcgg ccgtggcggc ggtggtggtg
     5761 atggtcatcg gctggcgcgc ggccgcgccg gtgcccgtct acgcgccgcc ggcgggcggg
     5821 cgcgagctcg ccggcatcgt catgctggtc gccttcgtcc tgttcccgac cgcgcgcctg
     5881 ccgtccaaca tccggcgctg ggtccgccat ccccagctca ccgcggtggc gctgtgggcg
     5941 gcggcgcacc tgatcgccaa cggcgactcg cggtccgtgc tcctgttcgg cgcactcggc
     6001 ctgtgggcgc tcatcgagat ggtgctgatc aaccgccg
//
LOCUS       DASWHU010000032        15056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1118699, whole genome shotgun sequence.
ACCESSION   DASWHU010000032 DASWHU010000000
VERSION     DASWHU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15056
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118699"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VGB88_01085"
     CDS             complement(<1..1059)
                     /locus_tag="VGB88_01085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9646059.1"
                     /translation="MSRLDRCHSIADLRRIARRRLPRGVFDYIDGGADDEVSLANNSA
                     AFAHLQLVPQVLTDVAEVDSSTEVMGRRIDMPLVLAPTGLSRLFHHLGERAVARAAAR
                     AGTVYSLSSASTVSIEDIGALTDGPKWFQIYVWRDRALLSDFMARCRAAGFDALCLTV
                     DVQVAGNRERDLRNGMALPPRLTARLALDVLRHPLWWANLLARPPLTLANVATSMQVE
                     GTGLTTMVDYVNAQFDRSVTWDDARWMIDQWGGPFAIKGILDPADAQRAVAAGATGII
                     VSNHGGRQLEHAPAPIEVLPEIAAAVAGRADVILDGGVRRGTDVLKAVALGAKACMAG
                     RPYLYGLAAGGEAGVDRAL"
     gene            1240..2310
                     /locus_tag="VGB88_01090"
     CDS             1240..2310
                     /locus_tag="VGB88_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9646060.1"
                     /translation="MSLALRELSADGAPAFDGHDRVVLAEHPESGLRAIIALHNTSRG
                     PAFGGCRMRPYRDLAEALDDALRLSRAMTYKSAICELPFGGGKAVIVGDPRTDKTPAL
                     LHAMGRVVDALAGAFLVAEDLGTSLRDMAAMRAVSAHVRGVADADGAPTPATAYGVFM
                     ALKAALAHRFGTADIAGRTVAVQGLGSVGARLCGYLAAAGATLVVADIDPAAVARVVA
                     KHPARPVDPDSIIAVPADIFAPCALGGVIDRRAVARLGAPIVVGAANNQLAAPELAEQ
                     LADAGIVWVPDYLANAGGVIDVAHEGPGYDPRAVLAACERLYDITGHVLDRAAAEGAT
                     PSRIADRLAEQRFTRAPRRRVA"
     gene            2390..2896
                     /locus_tag="VGB88_01095"
     CDS             2390..2896
                     /locus_tag="VGB88_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9646061.1"
                     /translation="MRGTGKLDRLDIAILAALQGDGRISNRALAQRVNLSPSACHART
                     RRLIEAGYVRRFGAEIDLDRVCANVEVHVEVTLEGHRHDEIAKFEKAVLALPEILECT
                     SVGGGHDYVLKVACADVRAYEALSQGLIDGGLGIKQLFSYITIRKVKRSEGYPLARLT
                     GATAGGRE"
     gene            complement(3066..4040)
                     /gene="speB"
                     /locus_tag="VGB88_01100"
     CDS             complement(3066..4040)
                     /gene="speB"
                     /locus_tag="VGB88_01100"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX9646062.1"
                     /translation="MADQGDRFQPIAGTVLPRFAGIATFMRLPHVPVEEAAGEVDIGF
                     VGVPWDGGTTNRPGARHGPRQMRDASTMMRKVHHATRIKPYELCRCADLGDVPVNPIN
                     VADTLGRVERFFDQVAAAAITPITAGGDHLVTLPILRALARARPVGLVHFDAHTDTWD
                     RYFGDERYTHGTPFRRAVEEGLLEPTRMIQIGIRGSMNADDEKDWTGAQGIRVIEIEE
                     YAELGPRKVVEEARRIVGDGPVYVSFDVDALDPVYAPGTGTPEIGGLSTFDCQVMLRG
                     LRGLDLVGGDVVEVSPPFDPSGNTALVGVTMMFEMLCLFAEAIAARRG"
     gene            4095..5135
                     /locus_tag="VGB88_01105"
     CDS             4095..5135
                     /locus_tag="VGB88_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9646063.1"
                     /translation="MKAAVFKAPGAPLAIEDVADPEPGPGDLILKVRACGICGTDIHW
                     SENRDHDVGWRVLEPGAVMGHEFAGEVVAVGREARAAWRVGDRVCAQPFIGCGTCAAC
                     RAGRGYCCAQVANRASLALPGAYAEYTRAGAAFSLRLPESVSFEQGALVEPLAVGLNA
                     VNKARLEAGDTALIVGAGPVGLSVALWCRFFGARHVVVSDLVARRAEAAHAFGASAAI
                     DASREDVPSRFEALTGGPPQVVFDCVGVPGSLQLAIDYAPVDARVVVVGLCMAEDRIF
                     PAKAITKELDLAFAFVYRRRDFDIVLDLLGRGRIDASALVTDHVGIDAFPAAFEALKA
                     PSDQIKVMLAPA"
     gene            5297..5599
                     /locus_tag="VGB88_01110"
     CDS             5297..5599
                     /locus_tag="VGB88_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9646064.1"
                     /translation="MKKRGVVYILASRPNGTLYVGVTSDLVRRVQQHKQGETGGFTSR
                     YGVTRLVYFEIYDDIEAAIVWEKRLKRWLRPWKIALIERTNPDWSDLWPNIAGEVE"
     gene            complement(5804..6733)
                     /locus_tag="VGB88_01115"
     CDS             complement(5804..6733)
                     /locus_tag="VGB88_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9646065.1"
                     /translation="MPARANQRVVYLNGEFVPESEALVPFRDRGFRYGDAVFDMTRTF
                     GHRIFKLKEHIERFYRSLRYVRIDPGLSPAEMTDITEEVLARNLHLLADDEDYWVGQR
                     ISRGVDVPGGDISAHAGPTVVVECTPLPLKPRARLFRDGIDVITPAVRRTPPDALSPN
                     AKTHNYLNLIMGDLEVKSRDAQAWAVTLDVNGNLCEGMGSNIFIVRAGELETPKVNYV
                     LPGVSRQTVIELAGTLDIEVKEHDISLFDAYIADEVFLTSTSLCICGVRSLNGARIDS
                     GAVPGPITRKLTDAYVDLVGFDFVNQYLSRLGD"
     gene            6933..8351
                     /locus_tag="VGB88_01120"
     CDS             6933..8351
                     /locus_tag="VGB88_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX9646066.1"
                     /translation="MTGRSLRLRWLLLGVAGVAIAAAALRAQEPGEARPVAVLSDLEG
                     PIGPAATRHVAKTLEAARERGAEVVILRINTPGGLAISMREIIAEILASPVPVVGFVA
                     PSGAHAASAGTYILYATHLAAMAPGTNLGAATPIMIGGGLPGLPSPAGEDKDGGKGDD
                     GEEGAPHGSLEAKAVEDAAALIRGLAEMHGRNAEWAEKAVREAASLSSSEALAAHVID
                     VVAPDVESLLAAIDGRTVTVGKTQHRLATAGLQLDHIEPDFLTKALGILANPNVAFML
                     MMLGVYGLIFEFANPGSIAPGVIGSISLILGLYALNQLPLDYAGLALVVLGIALMLAE
                     AFTPSFGVLGVGGIAAFVIGSAMLFDTDVPAYQLSWWVVGGTAGTSALVLMLMLHFVF
                     MSHRKDVVSGAEQMVGGEARVVDWTGETGYVRIEGERWRARSRRAFTPGQTVRVHGMD
                     GLTLLVDAAGERQGEDTEAEGG"
     gene            8354..9115
                     /locus_tag="VGB88_01125"
     CDS             8354..9115
                     /locus_tag="VGB88_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9646067.1"
                     /translation="MPLNYFVYAVVLLLLIIFFAAAIKIMREYERAVVFTLGRFTGVK
                     GPGLMIIIPIIQQIVRTDLRTVVEDVPTQDVISRDNVSVKVNAVIYFRIVDPQRAIIN
                     VEDYMSATSQLAQTTLRSVLGKHELDEMLAEREKLNADIQQILDEQTDAWGIKVANVE
                     IKHVDIDESMIRAIAKQAEAERLRRAKIINAEGEQQAAEKLVEAGEILAREPNAMLLR
                     YFAALHDIAGERSSTIVFPIPWGIMSRMFGAADAG"
     gene            complement(9131..10240)
                     /locus_tag="VGB88_01130"
     CDS             complement(9131..10240)
                     /locus_tag="VGB88_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9646068.1"
                     /translation="MNRSAWAMGIAGLAVGLVIGALVAGPGRAPGTTETADAVPGAPP
                     ADAGPVRWKMASTFPGTLTLLGESGKRLESRIALLSGGEIEIKFYEPGALVPAFEVFD
                     AVSAGSVDAGWSASGYWAGKMPAAQVFNSVPFGPLFQELMAWMRYGGGQKLYEELYEP
                     HNVHPIVCFFVPQDGSGWFRAPIASTADFAGLKIRFLGFGARVLEKFGASTQIIPGAD
                     IYTALELGTIDATEFSMPAIDLDLGFDQIAKHYYLPGWHQPANMGELIVNLARWRALD
                     ARRQALIEAACGESVVDSLASGEAAQADALTTIRAHGVTIHRWPPEMLEAFRKQWLVV
                     ADEVAAEDAEFASVFTSLQDFRAHYRIWHEYGRFE"
     gene            10468..11625
                     /locus_tag="VGB88_01135"
     CDS             10468..11625
                     /locus_tag="VGB88_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646069.1"
                     /translation="MTTYVRLATAVAVALSLPAAAQAAAMVTALGAGEGAVDIVAWPG
                     YIERGETDKAYDWVTDFEARTGCQVRVKTAGTSDEMVALMNEGGFDLVTASGDASLRL
                     VSGNRVQPVNTDLIPSWSAIDARLQNAPWHTVDGVHYGVPYQWGSNVLMYNTETFKEP
                     PASWAVVFKEAALPDGKPNKGRVQAFDGPIYIADAALYLMHTQPELGIKDPYELTEDQ
                     YQAALNLLRGQRQIVSRYWHDPFIQIDDFTNEGVVASSSWPFQVNLMVSQGKPIASTI
                     PAEGATGWADTTMMHANAPHPNCAYMWLEHSINPKLQGDLAAWFGSVPVNLAACQGNE
                     LLGDGGCATNGLGNFDQIQFWRTPVAQCATQGQCVPYYRWVTDYIAVLGGR"
     gene            11645..12706
                     /locus_tag="VGB88_01140"
     CDS             11645..12706
                     /locus_tag="VGB88_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017995225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646070.1"
                     /translation="MTRPRNAGARGAGGAGGLAPAVAVRFRAVSRHFGPVKAVDAVDL
                     EIREGEFFAMLGPSGSGKTTCLRLIAGFDQPSAGHIEIFGQTAEGVPPYRRAVNTVFQ
                     DYALFPHMNVLDNVGYALMVRGVPRAERERRARASLELVKLGGLEARRPAQLSGGQRQ
                     RVALARALVNEPRALLLDEPLGALDLKLREQMQDELKALQQTLGITFVFVTHDQGEAL
                     SMADRVAIFDAGRIVQVGSPEDVYERPRTRFVADFVGSSNVLAPDFAAAHGGARAWTS
                     LRPEKIRLVAPDGTAAASLSGTVAAVQYHGATTRLAVAVEGATLAVTTASAGCGVRPG
                     EAVSLAWEPAAMHVMEGEA"
     gene            12751..13644
                     /locus_tag="VGB88_01145"
     CDS             12751..13644
                     /locus_tag="VGB88_01145"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9646071.1"
                     /translation="MSDALILRPRLFLLILLAPPLLWLGIVYLGSLSALLLQSFFSID
                     EFSGVVRHQLTLKTYGELLQRANLDIILRSVLMAAAVTAAAVVIAFPVSYYAARHARG
                     RIKAAFYLAVMLPLWSSYLVRVYAWKLILAKEGILTWVVDRLGLGWALDALLAVPVIG
                     GPSLSISYIGTFLVFLYVWLPFMILPVQAALERVPASLLEASADLGARPRQTFRTVVL
                     PLALPGVVAGSIFTFSLTLGDYIIPQVIGSSRLFIGQAVFTHQGTAGNIPLAAAFTVV
                     PIVIMALYLWLAKRMGAFDAL"
     gene            13634..14446
                     /locus_tag="VGB88_01150"
     CDS             13634..14446
                     /locus_tag="VGB88_01150"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008883192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9646072.1"
                     /translation="MRSDRTPARLALKLAAAGGILFLHLPLALILLYAFTTEDKSYQF
                     PPPGLTAKWFAVAWGRPDIWQALWLSVRVAAISTAAALVLGTLAAAAVSRARFFGRET
                     ISLLIILPIALPGIITGISLRAAFNLIDIPFSFWTIVLGHATFCVVVVYNNALARFRR
                     TSASLIEASMDLGADGFQTFRHVVLPNIATALLAGGMLAFALSFDEVIVTTFTAGQQS
                     TLPIWMLSELVRPRQRPVTNVVAVFVIAVTFLPILAAYYLTRGGGEGAEQAK"
     gene            14463..>15056
                     /locus_tag="VGB88_01155"
     CDS             14463..>15056
                     /locus_tag="VGB88_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008883052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9646073.1"
                     /translation="MQTGMLIAGELVAGAGAPERVLNPRTGEAIVEVPEASADQVEAA
                     VGAAAKAFATWSRTTPGERAGLLLKLADRIEAEADAFAALEALNCGKPKHLVLADEIP
                     AIVDCLRFFAGAARCLPGSAAGEYLPGYTSMIRRDPVGVVASVAPWNYPLMMAAWKIA
                     PALAGGNTVVIKPSEQTPLTTLRLAECAAGILPEGVLN"
BASE COUNT         2143 a         5304 c         5333 g         2276 t
ORIGIN      
        1 gagggcgcgg tcgacgcccg cctcgccgcc ggccgcgagg ccgtacagat agggcctgcc
       61 ggccatgcac gccttggcgc cgagcgccac cgccttgagc acgtcggtgc cgcgccgcac
      121 cccgccgtcg aggatcacgt cggcgcgccc ggccaccgcg gcggcgatct cgggcagcac
      181 ctcgatcggc gccggcgcgt gctcgagctg gcggccgccg tggttggaga cgatgatgcc
      241 ggtggcgccg gcggcgaccg cgcgctgggc gtcggcggga tcgaggatgc ccttgatcgc
      301 gaacggcccg ccccactggt cgatcatcca tcgggcgtcg tcccaggtga ccgagcggtc
      361 gaactgggcg ttgacgtaat cgaccatggt ggtgaggccc gtgccctcga cctgcatgct
      421 ggtcgcgacg ttggcgagcg tgagcggcgg gcgggcgagc aggttcgccc accacagcgg
      481 atgccgcagc acgtcgagcg caagccgcgc ggtgagccgc ggcggcagcg ccatgccgtt
      541 gcgcaggtcg cgctcgcggt tgcccgccac ctgcacgtcg accgtgaggc acagcgcatc
      601 gaacccggcg gcgcggcagc gggccatgaa gtcgctgagc agcgcgcggt cgcgccagac
      661 atagatctgg aaccattttg ggccgtcggt cagcgcgccg atgtcctcga tcgacacggt
      721 cgaggccgag gacagcgagt agaccgtgcc cgcgcgcgcc gcggcccgcg ccaccgcccg
      781 ctcgcccaaa tggtggaaca ggcggctgag cccggtcggc gccagcacca gcggcatgtc
      841 gatgcgccgg cccatgacct cggtcgagct gtcgacctcg gcgacgtcgg tcagcacctg
      901 cggcaccagc tggagatgcg cgaacgcggc gctgttgttg gcgaggctca cctcgtcgtc
      961 ggcgccgccg tcgatgtagt cgaacacgcc ccgcggcagc cggcgcctgg cgatgcggcg
     1021 caggtcggcg atcgaatggc aacggtccag tcggctcaac gctcggctcc ttcccggtcg
     1081 cggcggcggc tcaccttata gcagagaccg cggctcgcca cccggcaatg gtgagcggcg
     1141 aacttcgtct cgaatcggac gcgccgggac gtacgaactg cggccctgcg ggcccgcttc
     1201 ggtgccctat ttcgcgcctt cggtgagata attttcccca tgagccttgc actccgagag
     1261 ctgtcggcgg acggcgcgcc cgcgttcgac ggccacgatc gggtggtgct cgccgagcac
     1321 cccgagagcg gcttgcgcgc gatcatcgcg ctccacaaca cgagccgcgg cccggcgttc
     1381 ggcggctgcc gcatgcgccc ctaccgggac ctggccgagg cgctcgacga cgcgctcagg
     1441 ctgtcgcggg cgatgaccta caagtcggcg atctgcgagc tgccgttcgg cggcggcaag
     1501 gcggtgatcg tgggcgatcc gcgcaccgac aagacgccgg cgctgctgca cgcgatgggc
     1561 cgggtcgtcg acgcgctcgc cggcgccttc ctggtcgccg aggacctcgg cacctcgttg
     1621 cgcgacatgg cggcgatgcg cgcggtctca gcccatgtgc gcggcgtcgc cgacgccgac
     1681 ggcgcgccca ccccggccac cgcctacggc gtgttcatgg cactcaaggc cgcgctcgcg
     1741 caccgcttcg gcaccgccga catcgccggc cgcacggtcg cggtgcaggg tctcggcagc
     1801 gtcggcgcgc gcctgtgcgg ctacctcgcc gcggccggcg cgactctggt ggtcgccgac
     1861 atcgacccgg ccgcggtcgc gcgcgtggtc gccaaacatc cggcgcgccc ggtcgatccc
     1921 gacagcatca tcgccgtccc ggccgacata ttcgcgccgt gcgcgctcgg cggggtcatc
     1981 gaccgccggg ccgtggcccg gctcggcgcg ccgatcgtgg tcggcgccgc caacaaccag
     2041 ctcgccgcgc ccgagctcgc cgagcagctc gccgacgccg gcatcgtctg ggtgccggat
     2101 tacctggcca atgccggcgg cgtgatcgac gtcgcccacg agggcccggg ctacgacccg
     2161 cgggcggtgc tcgcggcctg cgagcggctg tacgacatca ccggccatgt tctcgaccgt
     2221 gccgcggccg agggcgccac gccgagccgg atcgccgacc ggctggccga gcagaggttc
     2281 acgcgcgctc cccgccggcg cgtcgcctga tcgacgagcc ccgccgccgt ttgccggcgc
     2341 agccggcgcg gtctagtttg agcagggcga cgtttcagga gcggaacacg tgcgggggac
     2401 cggcaagctc gacaggctgg acatcgccat cctggctgcg cttcagggcg atgggcgcat
     2461 ctcgaaccgc gcgctcgccc agcgcgtcaa cctgtcgccg tcggcgtgcc acgcgcgcac
     2521 ccggcgcctg atcgaggccg gctatgtccg ccgcttcggc gccgagatcg acctcgaccg
     2581 cgtctgtgcc aatgtcgagg tccatgtcga ggtgacgctc gagggccacc gccacgacga
     2641 gatcgccaaa ttcgagaaag cggtgctcgc gctgccggag atcctcgaat gcaccagcgt
     2701 cggcggcggc cacgactacg tgctgaaggt ggcgtgcgcc gacgtgcgcg cctacgaggc
     2761 cctcagccaa gggctgatcg acggcggtct cggcatcaag cagctgttca gctacatcac
     2821 catccgcaag gtcaagcgat ccgagggcta tccactggcg cgcctgaccg gggccacggc
     2881 cggcgggcga gaatgagcgc gcgtgcctcg tactcgactg cgcaagaacc gcactcacgc
     2941 gacgaccctg ccggcctcct cagcgtaagg ccgttgttct gcaatgaata tcctcgtcct
     3001 gaggagcgag cggagcgagc gtctcgaagg acggcagcca catcacccgt ttgcgattcc
     3061 tgggcctagc cgcgccgtgc cgcgatcgcc tcggcgaaca ggcacagcat ctcgaacatc
     3121 atggtcacgc cgaccagcgc ggtgttgccg ctcgggtcga acggcggcga cacctcgacc
     3181 acgtcaccgc cgaccaggtc gaggccgcgc aggccgcgca gcatcacctg gcagtcgaag
     3241 gtgctgagcc cgccgatctc gggcgtgccg gtgcccggcg cgtagaccgg gtcgagcgcg
     3301 tcgacgtcga agctcacata gaccggcccg tcgccgacga tccggcgcgc ctcctcgacc
     3361 accttgcgcg ggccgagctc ggcatattcc tcgatctcga tcacccggat gccctgggcg
     3421 ccggtccagt ccttctcgtc gtccgcgttc atgctgccgc ggatgccgat ctggatcatg
     3481 cgcgtgggct cgagcaggcc ttcctcgacc gcgcgccgga acggcgtgcc gtgggtgtag
     3541 cgctcgtcgc cgaaatagcg gtcccaggta tccgtgtggg cgtcgaaatg gaccaggccg
     3601 acgggccgcg cgcgggccag cgcgcgcagg atcggcaagg tcaccagatg gtcgccgccg
     3661 gcggtgatcg gcgtgatcgc cgccgccgcg acctggtcga agaagcgctc gacgcggccc
     3721 aaggtgtcgg cgacgttgat cgggttgacc ggcacgtcgc cgagatcggc gcagcggcac
     3781 agctcgtacg gcttgatgcg ggtcgcgtga tgcaccttgc gcatcatggt cgaggcgtcg
     3841 cgcatctggc gcgggccgtg gcgcgcgccc gggcggttgg tggtgccgcc gtcccagggc
     3901 acgccgacga agccgatgtc gacctcgccc gcggcctcct cgaccggcac gtggggcagg
     3961 cgcatgaagg tggcgatgcc ggcgaagcgc ggcagcaccg tgcccgcgat cggctggaag
     4021 cggtcgccct ggtcggccat cgccggctat tctatcgcga ttggcggcgt cgccaagacg
     4081 gacgggaggc gaggatgaag gcagcggtgt tcaaggcgcc tggcgcgccg ctcgcgatcg
     4141 aggacgtggc cgaccccgag ccggggccgg gcgacctgat cctcaaggtg cgcgcctgcg
     4201 gcatctgcgg caccgacatc cactggtcgg agaaccgcga ccacgacgtc ggctggcggg
     4261 tgctggagcc gggtgcggtg atgggccacg aattcgcggg cgaggtggtg gcggtcgggc
     4321 gcgaggcgcg cgcggcctgg cgggtcggcg acagggtctg cgcccagccg ttcatcggct
     4381 gcgggacgtg cgccgcgtgc cgggcggggc gcggctactg ctgcgcgcag gtggccaacc
     4441 gcgccagcct cgccttgccc ggcgcctatg ccgagtacac ccgcgccggc gcggcgttca
     4501 gcctgcgcct gcccgaatcg gtgagcttcg agcagggcgc gctggtcgag ccgctcgcgg
     4561 tcgggctgaa cgcggtcaac aaggcgaggc tcgaggccgg cgacaccgca ctcatcgtcg
     4621 gcgccggccc ggtcgggctg tcggtggcgc tgtggtgccg gttcttcggc gcccgccacg
     4681 tcgtggtcag cgacctggtc gcgcgccgcg ccgaggccgc gcacgcattc ggcgcctcgg
     4741 ccgcgatcga cgcctcgcgc gaggacgtgc cgagccgctt cgaggcgctc accggcggcc
     4801 cgccgcaggt ggtgttcgac tgcgtcggcg tgcccggcag cctgcagctc gccatcgact
     4861 acgcgccggt cgacgcgcgc gtggtggtgg tcgggctgtg catggccgag gaccgcatct
     4921 tcccggccaa ggcgatcacc aaggagctcg acctcgcctt cgccttcgtc taccgccggc
     4981 gcgacttcga catcgtcctc gacctgctcg ggcgcgggcg catcgacgcc tcggcgctgg
     5041 tcaccgacca tgtcggcatc gacgccttcc ccgccgcctt cgaggcgctg aaggccccgt
     5101 ccgaccagat caaggtgatg ctcgcgcccg cctgacccga caccgcctgc gcgtcatgcc
     5161 ggccgagcgt aagcgagagc cggcatccat cgcgccgctg gcacgggcgc gtggaccccg
     5221 gataaccgct gcgcggtttc cggggtgacg gagagaggga gggccggcgg gactcgccgg
     5281 taggacttac cgggccatga agaagcgagg cgtcgtttac atattggcga gccgacctaa
     5341 cggtacgctc tacgtgggcg tgacctcaga cctcgtaagg cgcgtccaac agcacaagca
     5401 gggtgagact ggcggcttca caagtcggta tggagtgact cgcctcgttt acttcgagat
     5461 ttacgacgat atcgaagctg cgatcgtatg ggaaaagcga ctcaaacgat ggctccgacc
     5521 ttggaagatt gcgctgatcg agcgaaccaa tcccgactgg tccgacttgt ggccaaacat
     5581 cgcgggcgag gttgagtgag acctcgctgc gacggccggc gcgacagtat gcaatgcccc
     5641 ctcagcgggt cgtccaccca cctttgtcat gccggccgag cgtgagcgag agccggcatc
     5701 catcgcgccg ctggcacggg cgcgtggacc ccggataacc gctgcgcggt ttccggggtg
     5761 acgcgacaaa ttggggcggg agactgcgcg gccggcggct gtgtcagtcg ccgaggcggc
     5821 tcaggtactg gttgacgaag tcgaagccga ccaggtcgac ataggcgtcg gtcagcttgc
     5881 gggtaattgg tcccggcacc gcgcccgagt cgatcctggc gccgttgagg ctgcgcacgc
     5941 cgcagatgca caggctggtc gaggtcagga acacctcgtc ggcgatatag gcgtcgaaca
     6001 ggctgatgtc gtgctctttc acttcgatgt cgagcgtgcc ggcgagctcg atcaccgtct
     6061 ggcggctcac ccccggcagc acgtagttga ccttcggcgt ctccagttcg ccggcgcgga
     6121 cgatgaagat gttgctgccc atgccctcgc acaggttgcc gttgacgtcg agggtcaccg
     6181 cccacgcctg cgcatcgcgc gacttcacct cgaggtcgcc catgatcagg ttcaggtagt
     6241 tgtgggtctt ggcgttgggg ctgagcgcat cgggcggcgt gcggcgcacc gccggcgtga
     6301 tcacgtcgat gccgtcgcgg aacaggcgcg cgcgcggctt gagcggcagc ggcgtgcact
     6361 cgacgaccac ggtcggcccg gcgtgggcag agatgtcgcc gcccgggacg tcgacgccgc
     6421 gcgagatacg ctggccgacc cagtagtcct cgtcgtcggc gagcaagtgc aggttgcgcg
     6481 ccaacacctc ttcggtgata tccgtcatct ccgccgggct caggccggga tcgatgcgca
     6541 cgtagcggag cgaccggtag aagcgctcga tatgctcctt gagcttgaag atgcggtggc
     6601 cgaaggtccg ggtcatgtcg aacaccgcgt cgccgtagcg gaagccgcga tcgcggaacg
     6661 gcaccagcgc ctcgctttcg ggcacgaact cgccgttgag gtagacgact cgctggttgg
     6721 cgcgcgcagg catggccgga ctccttgtga ccgagggggc tcggcgccga tgctatgcgg
     6781 cagcgacgga tgcttgcaac ccgcgcggcg gctcggccca cctcacggcg gggtgatttt
     6841 cccgaacgcg gcaagatcgt gggcgctata ctcgcccccg cggggctccg cggcgggccg
     6901 tcggcgggcc tcgcgccggg aaggaggcgc cggtgaccgg ccggtcactt cgtcttcgtt
     6961 ggctgctgct cggggtcgcg ggcgtcgcga tcgcggccgc cgccctgcgc gcccaggagc
     7021 cgggcgaggc gcggcccgtg gcggtgctca gcgacctcga gggcccgatc ggtccggccg
     7081 cgacccgcca cgtcgcaaag accctggagg cggcacgcga gcgcggcgcc gaggtggtga
     7141 tcctgcgcat caacaccccg ggtgggctcg ccatcagcat gcgcgagatc atcgccgaga
     7201 tcctcgcctc gccggtgccc gtggtcggct tcgtcgcacc ctccggggcg cacgcggcaa
     7261 gcgccggcac ctacatcctc tacgccaccc accttgccgc gatggcgccg ggcaccaacc
     7321 tcggcgccgc caccccgatc atgatcggcg gcggcctgcc cggcctgccc tcgcccgccg
     7381 gcgaggacaa ggacggcggc aagggggatg acggcgagga gggggcgccg cacggctcgc
     7441 tcgaggccaa ggcggtcgag gacgccgccg ccttgatccg cggcctcgcc gagatgcacg
     7501 ggcgcaacgc cgagtgggcg gagaaggcgg tgcgcgaggc cgccagcctg tcgtcgagcg
     7561 aggcgctcgc tgcgcacgtc atcgacgtgg tcgcgcccga cgtggagtcg ctcctggcgg
     7621 cgatcgacgg gcgcacggtc acggtcggca agacgcagca caggctcgcc accgccgggc
     7681 tgcagctcga ccacatcgag cccgacttcc tgaccaaggc gctcggcatc ctcgccaacc
     7741 ccaacgtcgc cttcatgctg atgatgctcg gggtctacgg cctgatcttt gagttcgcca
     7801 accccggcag catcgcgccg ggcgtgatcg gctcgatctc gctgatcctc ggcctgtacg
     7861 cgctcaacca gctgccgctc gactatgccg ggctcgcgct ggtggtgctc ggcatcgcgc
     7921 tgatgctggc cgaggcgttc acgccctcgt tcggcgtgct cggggtgggc ggcatcgccg
     7981 cgttcgtgat cggctcggca atgctgttcg acaccgacgt gcccgcctac cagctgtcgt
     8041 ggtgggtggt cggcggcacc gccgggacca gtgcgctggt gctgatgctg atgctgcact
     8101 tcgtgttcat gagccaccgc aaggacgtgg tgagcggcgc cgagcagatg gtgggcggcg
     8161 aggcgcgggt ggtcgactgg acgggggaga ccggctatgt gcgcatcgag ggcgagcgct
     8221 ggcgcgcgcg cagccggcgc gcgttcacgc ccggccagac cgtgcgcgtc cacggcatgg
     8281 acgggctgac cttgctggtc gatgcggcgg gcgagcgcca aggggaagac accgaggccg
     8341 agggagggta gccatgccac tcaactattt cgtctatgcg gtcgtcctgc tgctgctgat
     8401 catcttcttc gccgcggcga tcaagatcat gcgcgagtat gagcgcgcgg tggtgttcac
     8461 cctcggccgc ttcaccggcg tcaaggggcc ggggctcatg atcatcatcc cgatcatcca
     8521 gcagatcgtg cgcacggatc tgcgcaccgt ggtcgaggac gtgccgaccc aggacgtgat
     8581 ctcgcgcgac aacgtgtcgg tcaaggtgaa cgcggtgatc tatttccgca tcgtcgaccc
     8641 gcagcgcgcg atcatcaacg tcgaggacta catgtcggcg accagccagc tcgcccagac
     8701 caccttgcgc tcggtgctcg gcaagcacga gctcgacgag atgctggccg agcgcgagaa
     8761 gctcaatgcc gacatccagc agatcctcga cgagcagacc gacgcctggg ggatcaaggt
     8821 cgccaacgtc gagatcaagc acgtcgacat cgacgagagc atgatccgcg cgatcgccaa
     8881 gcaggccgag gccgagcggc tgcgccgggc caagatcatc aacgccgagg gcgagcagca
     8941 ggcggccgag aagctggtcg aggcgggcga gatcctggcg cgcgagccga acgcgatgct
     9001 gctgcgctac ttcgccgcgc tccacgacat cgccggcgag cggtcgtcga cgatcgtgtt
     9061 cccgatcccg tgggggatca tgagccgcat gttcggcgcc gccgacgccg gctgaggccg
     9121 gagcgccagc tcactcgaac ctgccgtatt cgtgccagat gcggtagtgg gcgcggaagt
     9181 cctggagcga cgtgaacacg ctcgcgaact cggcgtcctc ggccgcgacc tcgtccgcca
     9241 ccaccagcca ctgcttgcgg aacgcctcca gcatctcggg cggccagcga tggatggtga
     9301 cgccgtgcgc ccggatcgtc gtcagcgcgt cggcctgcgc cgcctcgccg gaggcgagcg
     9361 agtcgaccac cgactccccg cacgcggcct cgatcagcgc ctgccggcgc gcatcaagcg
     9421 cgcgccagcg cgcgaggttg acgatcagct cgcccatgtt ggccggctgg tgccagcccg
     9481 gcaggtaata gtgcttggcg atctggtcga agccgaggtc gaggtcgatc gccggcatcg
     9541 agaactcggt ggcatcgatg gtgccgagct cgagcgcggt gtagatgtcg gcgcccggga
     9601 tgatctgggt cgaggcgccg aacttctcga gcacgcgcgc gccgaagccg agaaagcgga
     9661 tcttgaggcc ggcgaagtcg gcggtcgagg cgatcggggc gcggaaccag cccgagccgt
     9721 cctggggcac gaagaagcac acgatcggat gcacgttgtg cggctcgtac agctcctcgt
     9781 agagcttctg tccgccgccg tagcgcatcc acgccatcag ctcctggaac agcggcccga
     9841 aggggaccga gttgaacacc tgggccgccg gcatcttgcc ggcccagtag cccgacgccg
     9901 accagccggc gtcgaccgag ccggccgaga ccgcgtcgaa cacctcgaat gccggcacca
     9961 gcgcgccggg ctcgtagaac ttgatctcga tctcgccgcc ggacagcagc gcgatgcggc
    10021 tttccagccg cttgcccgac tcgccgagca gggtcagcgt gcccggaaag gtgctcgcca
    10081 tcttccagcg caccgggccg gcatcggccg gcggcgcgcc cgggaccgcg tcggcggttt
    10141 ccgtcgtgcc cggcgcccgg ccggggcccg cgaccagcgc cccgatcacc agccccacag
    10201 ccaggcccgc gatccccatt gcccaagccg accggttcat ggccatcccc cgtcgcagtc
    10261 gttaccgctc cgaacaagag tgtccgacca accgtcgggg tcaaggacgc tgcgcccgac
    10321 ccgatcgcgc cggctcgttt cgcgggccgc ggccggcgat cttttgattg gcggacccgg
    10381 gccgagttgc ggcacactcc ccgccttgtg cgcaggccgc gtcgtgtgcg gcgcaggcat
    10441 tgcgcgggcg aaggggaggg taccgtcatg acaacctatg tccgtctcgc gaccgcggtg
    10501 gcggtcgccc tgtcgttgcc ggccgcggca caggccgcgg ccatggtgac cgcgctcggc
    10561 gccggcgagg gcgcggtcga catcgtcgcc tggcccggct acatcgagcg cggcgaaacc
    10621 gacaaggcct acgactgggt caccgacttc gaggccagga ccggctgcca ggtccgggtc
    10681 aagaccgccg gcacctcgga cgagatggtg gcgctgatga acgagggcgg cttcgacctg
    10741 gtcaccgcct cgggcgacgc gtcgctcagg ctggtctccg gcaaccgcgt gcagccggtc
    10801 aataccgacc tgatcccgtc ctggagcgcg atcgacgcgc ggctgcagaa cgcgccctgg
    10861 cacacggtcg acggcgtcca ttacggcgtg ccgtaccagt ggggctcgaa cgtgctgatg
    10921 tacaacaccg agacgttcaa ggagccgccg gcgagctggg cggtggtgtt caaggaggcg
    10981 gcactgcccg acggcaagcc gaacaagggc cgggtgcagg cattcgacgg gccgatctac
    11041 atcgccgacg ccgcgctcta cctgatgcat acccagcccg agctcggcat caaggacccc
    11101 tacgagctca ccgaggacca gtaccaggcg gcgctcaacc tgctgcgcgg ccagcgccag
    11161 atcgtcagcc gctactggca cgacccgttc atccagatcg acgacttcac caacgagggc
    11221 gtggtggcat cgtcgtcatg gccgttccag gtcaacctga tggtcagcca aggcaagccg
    11281 atcgcatcca cgatcccggc cgagggcgcc accggctggg cggacacgac catgatgcac
    11341 gccaacgcgc cgcatccgaa ctgcgcctat atgtggctcg agcactcgat caatcccaag
    11401 ctgcagggcg acctcgcggc atggttcggc tcggtgccgg tcaacctcgc cgcatgccag
    11461 ggcaacgagc tgctcggcga cgggggctgc gcgaccaacg gcctcggcaa cttcgaccag
    11521 atccagttct ggcggacgcc ggtcgcgcag tgcgcgacgc aggggcaatg cgtgccgtac
    11581 taccggtggg tgaccgacta catcgcggtg ctcggcggcc gctgagcggc cgccaggctg
    11641 cggcgtgaca cgcccccgga acgccggcgc gcgcggcgcc ggcggcgccg ggggccttgc
    11701 gccggcggtc gcggtacgct tccgggcggt ctcgcgccat ttcgggccgg tcaaggcggt
    11761 cgatgcggtc gacctcgaga tccgcgaggg cgagttcttc gccatgctcg gcccgtcggg
    11821 ctcgggcaag accacctgcc tgcgcctgat cgccggcttc gaccagccgt ccgccggcca
    11881 tatcgagatc ttcggccaga ccgccgaggg cgtgccgccc taccggcgcg cggtcaatac
    11941 ggtgttccag gactacgcgc tgttcccgca catgaacgtg ctcgacaacg tcggctacgc
    12001 gctgatggtg cgcggggtac cgcgagccga gcgcgagcgg cgggcgcgcg cctcgctcga
    12061 gctggtcaag ctcggcgggc tcgaggcgcg gcgcccggcg cagctgtcgg gcggccagcg
    12121 ccagcgggtg gcgctggcgc gcgcgctggt caacgagccg cgcgcgctgc tgctcgacga
    12181 gccgctcggc gccctcgacc tcaagctgcg cgagcagatg caggacgagc tcaaggcgct
    12241 ccagcagacg ctcggcatca ccttcgtgtt cgtcacccac gaccagggcg aggcgctgtc
    12301 gatggccgac cgggtggcga tcttcgatgc cgggcgcatc gtccaggtgg gcagcccgga
    12361 ggacgtctac gagcgcccgc gcacccgctt cgtcgccgac ttcgtcggct cgtcgaacgt
    12421 gcttgcgcct gatttcgccg ccgcccacgg cggcgcgcgc gcctggacca gcctcaggcc
    12481 ggagaagatt cgcctggtcg cgccggacgg cacggccgcg gcgagcctgt cggggacggt
    12541 cgccgcggtc cagtatcacg gcgcgacgac gcggctcgcg gttgcggtcg agggcgcgac
    12601 gctcgcggtc accaccgcct cggccggctg cggcgtgcgc ccgggcgagg cggtgagcct
    12661 ggcctgggag cctgcggcga tgcacgtcat ggagggcgag gcgtgattcg cccggcgttc
    12721 gcaggcgccg gcggcggcag gctcagcggc ctgtcggacg cgctgatcct ccgcccgcgg
    12781 ctgttcctgc tcattctgct cgcgccgccg ctgttgtggc tcggcatcgt ctatctcggc
    12841 tcgctgtcgg cgctgctgct gcaatcgttc ttctcgatcg acgagttctc gggcgtggtc
    12901 cgccaccagc tcacgctcaa gacctacggc gagctcctcc agcgcgccaa cctcgacatc
    12961 atcctgcgct cggtgctgat ggccgcggcg gtgacggcgg cggcggtggt gatcgcgttc
    13021 ccggtttcct attacgccgc ccgccatgcg cgcgggcgga tcaaggcggc gttctacctc
    13081 gcggtcatgc tgccgctgtg gtcgagctac ctggtgcggg tttatgcctg gaagctgatc
    13141 ctggccaagg aggggatcct gacctgggtg gtcgaccgcc tcggcctcgg ctgggcgctg
    13201 gacgcgctgc tcgcggtgcc ggtgatcggc gggccgtcgc tgtcgatcag ctacatcggc
    13261 accttcctgg tgttcctcta cgtctggctg ccgttcatga tcctgccggt ccaggccgcg
    13321 ctcgagcgtg tgccggcgag cctgctcgag gcctcggccg atctcggcgc acgcccgcgc
    13381 cagaccttcc gcaccgtggt gctgccgctc gcgcttccgg gcgtggtcgc gggctcgatc
    13441 ttcaccttct cgctgaccct tggcgactac atcatcccgc aggtgatcgg ctcctcgcgc
    13501 ctgttcatcg gccaggcggt gttcacccat cagggcaccg ccggcaacat cccgctcgcc
    13561 gccgcgttca cggtggtgcc gatcgtgatc atggcgctgt atctgtggct ggccaagcgc
    13621 atgggggcgt tcgatgcgct ctgaccggac gccggcgcgc ctcgcgctca agctcgccgc
    13681 cgcgggcggg atcttattct tgcacctgcc gctcgccctg atcctgctct acgccttcac
    13741 caccgaggac aagagctacc agttcccgcc gcccgggctg accgccaagt ggttcgcggt
    13801 cgcctggggg cgcccggaca tctggcaggc gctgtggctt tcggtcaggg tcgccgcgat
    13861 ctcgaccgcc gcggcgctgg tgctcggcac actcgccgcc gcggcggtgt cgcgggcgcg
    13921 attctttggc cgggagacga tttccctgct catcatcctg ccgatcgcgc tgcccgggat
    13981 cataaccggc atctcgctgc gcgctgcgtt caacctgatc gacatcccat tctcgttctg
    14041 gaccatcgtg ctggggcacg cgacgttctg cgtggtggtg gtctacaaca acgcgctcgc
    14101 ccgtttccgg cgcacctcgg cgtccttgat cgaggcctcg atggaccttg gcgccgacgg
    14161 tttccagacc ttccgccacg tcgtgctgcc gaacatcgcc accgcgttgc tcgccggcgg
    14221 catgctcgcc ttcgcgctct ccttcgacga ggtcatcgtc accaccttta ccgccgggca
    14281 gcagtcgacc ttgccgatct ggatgctgag cgagctggtg cgcccgcgcc agcgcccggt
    14341 caccaacgtc gtcgcggtgt tcgtcattgc cgtcaccttc ctgccgatcc ttgcggccta
    14401 ctacctgacg agaggcggcg gcgagggcgc cgagcaggcc aaataagggg aggatgtgcg
    14461 ccatgcagac cgggatgctg atcgcgggcg agctggtcgc cggcgcgggc gcgcccgagc
    14521 gggtgctgaa cccgaggacc ggggaggcga tcgtcgaggt gcccgaggcg tcggccgacc
    14581 aggtcgaggc cgcggtcggc gcggcggcga aggccttcgc gacctggtcc aggaccacgc
    14641 cgggcgagcg cgcgggcttg ctgctcaagc tcgccgatcg gatcgaggcc gaggccgacg
    14701 ccttcgccgc gctcgaggcg ctcaactgcg gcaagcccaa gcatctcgtg ctcgccgacg
    14761 agattccggc gatcgtcgat tgcctgcgct tcttcgccgg cgccgcgcgc tgcctgccgg
    14821 gcagcgccgc gggcgaatac ctgccaggct acacgtcgat gatccgccgc gacccggtcg
    14881 gggtggtggc ctcggtcgcg ccgtggaact acccgctgat gatggcggcg tggaagatcg
    14941 ccccggcgct cgccggcggc aacacggtgg tgatcaagcc gtccgagcag acgccgctga
    15001 ccacgctcag gctcgccgag tgcgccgccg gcatcctgcc cgaaggcgtg ctcaac
//
LOCUS       DASWHU010000033         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1488256, whole genome shotgun sequence.
ACCESSION   DASWHU010000033 DASWHU010000000
VERSION     DASWHU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488256"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="modC"
                     /locus_tag="VGB88_01160"
     CDS             complement(<1..1028)
                     /gene="modC"
                     /locus_tag="VGB88_01160"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEX9646074.1"
                     /translation="MISLAVRSAIGTFSLDIRLDLPEAGITALFGRSGAGKTSLLDII
                     AGLRRPDCGHIAVHGRVLYDSDAGIDVPAERRRVGYIFQDGRLFPHLSVRANLVYGMK
                     RTPPGERQVGFDQVVGLLGIASLLERRPRGLSGGEKQRVAIGRALLTSPRLLLLDEPL
                     AALDPARKAEVLPFIERLHTELGVPMVYVSHALDEVLAIADTMALIDDGRVRAVGPVE
                     QIASSLELVADTGFAEAGTVIAATVARHDEGHGVTELDSAAGVLRVGRLDAAVGARIR
                     LRVRARDVALALQPPRDISVLNILPGTVARIAARDSGAVEVAVEVAPGLLLWSEITRL
                     AAARLALAP"
     gene            complement(1032..1724)
                     /gene="modB"
                     /locus_tag="VGB88_01165"
     CDS             complement(1032..1724)
                     /gene="modB"
                     /locus_tag="VGB88_01165"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX9646075.1"
                     /translation="MPPLGPEEWQALALSLRVGAWSVAVSLPLGIALAWLLCRRSFPG
                     KSLVDGLVHLPLVLPPVVVGYALLVLLGRKGVIGEPLYDLLGITVAFKWQGAAVASAV
                     MGFPLMVRAIRLSLEAVDRGLEDAARTLGAGPMSVFLTVTLPLIMPGVLTGAILSFAR
                     SLGEFGATITFVSNIPGETRTLPVALYTYTQVPGGDAPALRLAAISLVLALGALLASE
                     LLARRIQVRLGR"
BASE COUNT          285 a          641 c          644 g          191 t
ORIGIN      
        1 ggcgcgagcg cgaggcgggc ggcggcgagc cgcgtgatct cggaccacag aagcagcccc
       61 ggcgcgacct cgacggcgac ctcgacggcg ccggaatcgc gcgccgcgat gcgcgccacc
      121 gtgccgggga ggatgttgag cacgctgatg tcgcgcggcg gctgcagcgc gagcgccacg
      181 tcgcgcgcac gcacgcgcag gcgtatgcgc gcgccaacgg cggcatcgag ccgcccgacc
      241 ctgagcacgc ccgcggccga gtcgagctcg gtgacgccgt ggccctcgtc gtggcgcgcc
      301 accgtggcgg cgatcaccgt gccggcctcg gcaaagccgg tgtcggcgac cagctcgagg
      361 ctgctcgcga tttgttcaac gggcccgacc gcgcgcaccc ggccgtcgtc gatcagcgcc
      421 atggtgtcgg cgatcgcaag cacctcgtcg agcgcgtggc tgacatagac catgggcacg
      481 ccgagctcgg tgtgcaggcg ctcgatgaac ggcagcacct ccgccttgcg ggcgggatcg
      541 agcgccgcca gcggctcgtc gagcagcaac agccgcgggc tggtgagcag cgcccggccg
      601 atcgccaccc gctgcttctc gccccccgac agccccctcg gccggcgctc gagcaggctg
      661 gcgatgccga gcagcccgac cacctggtcg aagccgacct ggcgctcgcc gggcggcgtc
      721 cgcttcatgc cgtagaccag gttggcgcgc accgacaaat gcgggaacag gcgcccgtcc
      781 tggaagatgt agccgacgcg acggcgctcg gccggcacgt cgatgccggc gtccgagtcg
      841 tagaggacgc ggccgtggac cgcgatatgg ccgcaatcgg ggcgcctgag gccggcgatg
      901 atatcgagca gcgaggtctt gccggcgcca gagcggccga acagggcggt gatcccggcc
      961 tcgggcagat cgaggcggat gtcgagcgag aacgtcccga tcgcgctccg gaccgccagc
     1021 gagatcacgt ctcagcgccc gagccgcacc tggatgcgcc gcgccagcag ctccgaggca
     1081 agcagcgcgc cgagcgccag caccagcgat atcgccgcca ggcgcagcgc cggcgcatcc
     1141 ccgcccggga cctgggtgta ggtgtagagc gccaccggca gggtgcgcgt ctcgcccggg
     1201 atgttggaga cgaaggtgat ggtggcgccg aactcgccca ggctgcgcgc gaacgacagg
     1261 atcgcgccgg taagcacgcc cggcatgatc agcggcaggg taacggttag gaagacactc
     1321 atcggccccg cgccgagggt gcgcgcggcg tcctctaggc cgcgatcgac cgcttccagc
     1381 gacagccgga tcgcgcgcac catgagcggg aagcccatca ccgccgaggc caccgccgcg
     1441 ccctgccact tgaacgcgac cgtgatgccg agcagatcgt agagcggctc gccgatcacg
     1501 cccttgcgcc cgagcaggac caggagcgcg tagccgacca ccaccggcgg cagcaccagc
     1561 ggcaggtgga cgaggccgtc gaccaggctc ttgccgggaa agctccgccg gcacagcagc
     1621 caggcgaggg cgatgccgag cggcaggctc acggcgacgc tccaggcgcc gacccgcagg
     1681 ctgagcgcga gcgcctgcca ctcctccggc ccgagcggcg gcaaggctca gtcgaccgcg
     1741 aagccgtagc gggcgaagat c
//
LOCUS       DASWHU010000034        10890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2599379, whole genome shotgun sequence.
ACCESSION   DASWHU010000034 DASWHU010000000
VERSION     DASWHU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599379"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="ccmB"
                     /locus_tag="VGB88_01170"
     CDS             <1..622
                     /gene="ccmB"
                     /locus_tag="VGB88_01170"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX9646076.1"
                     /translation="LRRAGDTVAVLAFFVIAIVLFPLGVGPEAELLARIAAGTIWVAA
                     LLAAMLSLDRLFAADHDDGSLDLLLLVPLPLPAVVLAKCAAHWLTTGLPLMVAAPLLA
                     VLMSLPLAGFAALLAAMALGTPTLSLIGAIGAALTVGVRRGGVLVPLLVLPLVIPVLI
                     FGVAAVDGAVAGTGAGGQLKVLGALLAGALALAPWAAAAGLRLAAE"
     gene            652..1419
                     /locus_tag="VGB88_01175"
     CDS             652..1419
                     /locus_tag="VGB88_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX9646077.1"
                     /translation="MHRFANPSRFMRLADAVLPWAVGVAALLVPLGLYLALIASPPDY
                     QQGESVRIMYIHVPAAWMALFVYVLMAAASAAGLIWRHPLADIAARAASPIGAGFTFV
                     ALCTGALWGQPMWGTWWVWDARLTSVLILFFLYLGHMALYDAFDDATKGARAAAILAL
                     VGVVNVPIIHYSVEWWNTLHQPASILRGGGPSIDASMLWPLFVMMGAFKAYFLAVLLV
                     RMKSELLAARIRGLRVSQVQAAELAPGAAPVSGAETG"
     gene            1416..1604
                     /gene="ccmD"
                     /locus_tag="VGB88_01180"
     CDS             1416..1604
                     /gene="ccmD"
                     /locus_tag="VGB88_01180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX9646078.1"
                     /translation="MSALAGFLAMGGYAQFVWPAYAVAAAVLAGLALAAARRLRARRA
                     ELAALEARVGTARRRGRA"
     gene            1601..2062
                     /gene="ccmE"
                     /locus_tag="VGB88_01185"
     CDS             1601..2062
                     /gene="ccmE"
                     /locus_tag="VGB88_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX9646079.1"
                     /translation="MRTPFKRKTLRLYAVLAGLVLLGTAAALALSAFEDNLVFFYGPT
                     ELAELGIGPGRTVRIGGLVEEHSVRRLDDGLSVEFRVTDLRTTLPVRFTGVLPDLFRE
                     GQGVVAEGVLGGDGTFVARQVLAKHDETYMPPEVAETLRKSGHWQGEEAAR"
     gene            2059..4038
                     /locus_tag="VGB88_01190"
     CDS             2059..4038
                     /locus_tag="VGB88_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX9646080.1"
                     /translation="MIVETGHFALALALVVALVQASLPLIGAQRGEAAWMALARPAAA
                     VQLAAVAAAFAALTHAYVVSDFSVANVVANSHTAKPLLYKLTGVWGNHEGSLLLWVLI
                     LALFGAAVAAFGGNLPPALRARVLAVQAMIAVGFLAFMLFTSNPFARAFPPPLDGNDL
                     NPLLQDPGLAFHPPFLYLGYVGFSITFAFAIAALIEGRVDATWARWVRPWALAAWSFL
                     TVGIALGSWWSYYELGWGGWWFWDPVENASFMPWLAGTALLHSSIVCEKREALKSWTV
                     LLAIVAFSFSLLGTFLVRSGVLTSVHAFATDPARGVFILAFLVLVIGGSLALYAVRAP
                     RLRAGGLFAPLSREGLLVLNNLLFATAAATVLLGTLYPLFVDALGAGKVSVGPPYFNA
                     VFIPLMAPLIALAGLTPLMPWKRGDLAGVLGRLKAAVAAAGLAALAVLVLADRGGLLA
                     ALGIALAAWLAASVAVEWASRVRLLRAPLAESLGRARALPRAAYGMSLAHLGAAIMVA
                     GITASSSWQVESFQVMRPGESVTVAGYDWRFHGVEALDGPNYSITRARLTVSRDGREV
                     AEMLPEKRFYPVARMPTTEAAIHTTWLADLYAVIGDGDGEGGFSTRLYYNPLVPWIWL
                     GAIVMALGGATSLTDRRHRVGVPRRAQAHAAGARA"
     gene            4043..4582
                     /locus_tag="VGB88_01195"
     CDS             4043..4582
                     /locus_tag="VGB88_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX9646081.1"
                     /translation="MAARHWLYVVPAAVFATLAGLLYWGLFQDPSQVPTVMAGRTLPA
                     FDLPPVDGATPGLASSDVKTGEISVINVFASWCVPCRAEHPQLTEIAALEGVHLYGID
                     YKDTPEDVRAFLDDLGDPYARIGADADGRVGIDLGVYGVPETYFVGRDGLIHYKHIGP
                     IAPEHMTKRIRPLIERLKQ"
     gene            4579..5064
                     /locus_tag="VGB88_01200"
     CDS             4579..5064
                     /locus_tag="VGB88_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX9646082.1"
                     /translation="MTRVARRLAGMVLAALMVGVAATAALAFQTDTPLADPAQEARAV
                     ALAKELRCLVCQNQSIMESDADLAKDLRGIVRERIAAGDSDAQVKQYVVERYGDFVLL
                     RPPFKAKTWALWLGPALIAAAALAALAAVWRRRASRAQGPAPLTEAERARLRDLLGDD
                     A"
     gene            5061..6461
                     /gene="ccmI"
                     /locus_tag="VGB88_01205"
     CDS             5061..6461
                     /gene="ccmI"
                     /locus_tag="VGB88_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX9646083.1"
                     /translation="MSAAAWALFGALLVAAVAVLARAALAGARRGGGEARAAYDLQIY
                     RDQLAELDNDLDRGVVSPEQAQAARTEIERRVLALGTAPDRGPARAGRRTGGVTAGAL
                     AIAVPALTALIYALVGTPGAPDMPLAARESVAPPEMPADVEEAVGRLAARLESEPDDL
                     QGWLLLGRSYAFLERYDQAAVAFGTAAALVPDDTEVAAAYGEALLFAADGVVTPAARE
                     QFEAVLARDAKHEGARLYLGMAAAQAGELERAFDIWRALAEDADGSEPWLAALRAQLM
                     ALSRELGVEAPAVALEAAPTAPAAEAPGPGPEDLEMAAGMSPDERMAAIDSMVARLEG
                     RLIEHPDDPDGWKRLGRAKRVLGDLAGARAAYGEAVKRAPADGAALAGLAMVMVEQTG
                     PETVTPEAADLFRRALALEPANGEALWFLGLLAAQSGDADQAVAMWRRLLGQLAPGSD
                     EHRMLSRRIDELAAKE"
     gene            6581..7462
                     /locus_tag="VGB88_01210"
     CDS             6581..7462
                     /locus_tag="VGB88_01210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9646084.1"
                     /translation="MSVARAADPADPGRDFADFWNRVLAPKFIRYKHIMVDGLTHHSE
                     AIFPSLPVRQGDAVLDVGCGFADTAIRLARTVGARGRVAGIDCCAAFLDVGRADVAAA
                     GLSNVTLILGDAQSYPFEPEYDFVFSRFGTMFFENPVWGLRNMRTALKPGAAMTMIVW
                     RTIDDNPWLGLPKKVVLDFLPPPGESAETCGPGPFSMANRDTVTGMLKAAGYLEPAFK
                     RVDAPVRVGKTVKDSTDFQLALGPAGEVFREAGELAERRRAEIEAALGEAIAPYQTDE
                     GVVMQSSSWVVTARNPG"
     gene            complement(7511..9436)
                     /locus_tag="VGB88_01215"
     CDS             complement(7511..9436)
                     /locus_tag="VGB88_01215"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9646085.1"
                     /translation="MDIVAMADTGTKRKLAAILSADVAGYSRLMASDEAATVRALNQA
                     RARFRERIAARGGRVVDTAGDSVLAEFASVVEAVECALEVQAAQAESNAPLPADRRML
                     FRIGVNLGDVIAQDDGTIYGDGVNIAARLEALAEPGTVVVSESAHMQVRGKLDVGFLD
                     GGTHKVKNIAEPVRAWRVTDAADGTGGRPASAARGRRRLRAGAAALVAVVAAVAALWA
                     IERPTPTAGRNAGPDTVPAAASALALTVPDGPSIAVLPFTNMSEEAEQEYFSDGLTED
                     IITELSRIHGLFVIARNSTFQYKGRSVDVPEVARAFGVRYVVEGSVRRAGEQVRVTAQ
                     LIDAESGGHVWAERYDRELTDIFAVQDDITRQIVAALELELAGPASPAATRKLTDNVE
                     AYDYFLRGRAYKDRTTRETNRLAEEMLDKAIALDPGFAGAYAELAYVFFRDWAYSWTD
                     DADTLNKAAAAAEKAVALDPDLPLALARLAWVKSWQRQHDVAIAAGLKAIALDPNYAD
                     AYIMLSGIYVFAGEPENAIAMAEKAMRLDPNVRFDLLMQVGHAHFLAGRHQQAVEFLK
                     RSLERNPDFPAAHQILAAAYSELGRDEDGRAELAEVQRLSPKGGIDPERVPYKDPATL
                     RRVWAAIDRLGARPPTD"
     gene            complement(9693..10274)
                     /locus_tag="VGB88_01220"
     CDS             complement(9693..10274)
                     /locus_tag="VGB88_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646086.1"
                     /translation="MVWPIFGDHDVKFEAITHLMSSADDIVVAIIGGTFLEEAVERTL
                     KERMRDNKDVSDRLFDLTKPLSSTSAQIDVLYMLWGIESHVRETMLGICRIRNFFAHN
                     MGVNFDSDDPKFTKHLPKLNLHERHETYPDARFGTPTSKKIKTPKNNRETFIVNLQLC
                     LNYLMQDRCKHIAYSTQLVSYEELRKKVGKPWV"
BASE COUNT         1466 a         3763 c         3873 g         1788 t
ORIGIN      
        1 gctcaggcgg gcgggcgaca cggtggcggt gctcgccttc ttcgtcatcg ccatcgtgct
       61 gttcccgctc ggcgtcgggc ccgaggccga gctgctggcg cggattgccg ccggcaccat
      121 ctgggtggcg gcgctgctcg cggcgatgct gtcgctcgac cgcctgttcg ccgccgacca
      181 cgacgacggc agcctcgacc tgctgctgct cgtgcccctg ccgctgccgg cggtggtgct
      241 ggccaagtgc gccgcccatt ggctgaccac ggggctgccg ctgatggtcg cggcgccgct
      301 gttggcggtg ctgatgagcc tgccgctcgc cggcttcgcc gccctcctcg cggcgatggc
      361 gctcggcacg cccacgctca gcctgatcgg cgccatcggc gcggcgctga cggtgggggt
      421 gcgccgcggc ggggtgctgg tgccgctgct ggtcttgccc ttggtgatcc cggtgctgat
      481 attcggggtg gccgcggtcg acggcgcggt cgcgggcacc ggcgcgggcg gccagcttaa
      541 ggtgctgggc gcgctgctcg ccggcgcgct tgcgctcgcc ccgtgggcgg cggcggccgg
      601 cctgcgcctg gccgcggaat gagacgagga gaggcccgga tggcgtccac cctgcaccgg
      661 ttcgccaacc ccagccggtt catgcgcctg gcggatgcgg tgctgccgtg ggccgtgggc
      721 gtggccgcgc tcttggtccc gctcggtctg tacctggcgc tgatcgcctc gccgcccgac
      781 taccagcagg gcgagtcggt gcgcatcatg tatatccacg tgccggccgc gtggatggcg
      841 ctgttcgtct acgtgctgat ggcggctgcg agcgcggccg ggctgatctg gcggcacccg
      901 ctcgccgaca tcgcggcgcg cgccgcctcg ccgatcggcg ccggcttcac cttcgtcgcg
      961 ctgtgcaccg gcgcgctgtg gggccagccg atgtggggca cctggtgggt gtgggacgcg
     1021 cggctgacct cggtgctgat cctgttcttc ctctatctcg gccacatggc gctgtacgac
     1081 gccttcgacg acgccaccaa gggcgcgcgc gcggcggcga tcctggcgct ggtcggcgtg
     1141 gtcaacgtgc cgatcatcca ctactcggtg gagtggtgga acacgctcca tcagccggcg
     1201 agcatcctgc gcggcggcgg gccgtcgatc gatgccagca tgctgtggcc gctgttcgtg
     1261 atgatgggcg cgttcaaggc ttatttcctc gccgtgctgc tggtgcgcat gaagtcggag
     1321 ctgctcgccg cgcgcatccg cggcctgcgc gtgagccagg tgcaggcggc ggagctcgcg
     1381 cccggggcgg cgccggtatc gggagccgag acgggatgag cgcgctggcc gggttcctgg
     1441 cgatgggcgg ctacgcccag ttcgtgtggc cggcctacgc ggtggcggcg gcggtgctcg
     1501 ccgggctcgc gctggcggcg gcaaggcgcc tgcgcgcgcg ccgcgccgag ctcgccgcgc
     1561 tcgaggcgcg ggtgggcacg gcgcgccgca gggggcgggc gtgaggacgc ccttcaagcg
     1621 caagacgctc cgcctgtacg cggtgctcgc cggcctggtc ctgctcggga ctgcggcggc
     1681 gctggcgcta agcgcgttcg aggacaacct ggtgttcttc tacggcccca ccgagctcgc
     1741 cgagctaggc atcgggcccg gccggacggt gcgcatcggc ggcctggtcg aggagcacag
     1801 cgtccgccga ctcgacgacg ggctgtcggt cgagttccgg gtcaccgacc tccggaccac
     1861 attgccggtg cgattcaccg gggtgctgcc cgacctgttc cgcgaaggcc agggcgtggt
     1921 cgccgagggc gtgctgggtg gcgacggcac cttcgtcgcc cgccaggtgc tggccaagca
     1981 cgacgagacc tacatgccgc ccgaggtcgc cgagacgctc aggaagtccg gccactggca
     2041 gggcgaggag gcggcgcgat gatcgtcgag accggacatt tcgcgctcgc gctcgcgctg
     2101 gtggtggcgc tggtgcaggc gagcctgccg ctgatcgggg cgcagcgcgg cgaggcggcg
     2161 tggatggcgc tggcgcggcc cgcggcggcg gtgcagctcg cggcggtggc ggcggcgttc
     2221 gccgcgctca cccacgccta cgtcgtctcc gacttctcgg tcgccaacgt ggtcgccaac
     2281 tcgcacaccg ccaagccgtt gctctacaag ctcactggcg tatggggcaa ccacgagggc
     2341 tcgctgctct tgtgggtgct gatcctggcg ctgttcgggg ctgcggtcgc ggccttcggc
     2401 ggcaacctgc cgccggcgtt gcgggcgcgc gtgctcgccg tccaggcgat gatcgcggtg
     2461 ggcttcctcg cctttatgct gttcacctcg aacccgttcg cgcgcgcctt cccgccgccg
     2521 ctcgacggca acgacctcaa cccgctgctg caggatcccg gcctcgcctt ccacccgccg
     2581 ttcctctatc tcggctatgt cggcttctcg atcaccttcg cgttcgcgat cgcggccctc
     2641 atcgaaggcc gggtcgacgc cacctgggcg cgctgggtga ggccgtgggc gctggccgcg
     2701 tggagcttcc tcaccgtcgg catcgcgctc ggcagctggt ggtcctatta cgagctcggc
     2761 tggggcggct ggtggttctg ggacccggtc gagaacgcct cgttcatgcc ctggctcgcg
     2821 ggcaccgcgt tgctccactc ctcgatcgtg tgcgagaagc gcgaggcgct caagtcgtgg
     2881 accgtgctgc tcgccatcgt cgcgttctcg ttcagcctgc tcggcacctt cctggtgcgc
     2941 tcgggcgtgc tgacctcggt ccacgccttc gccaccgacc cggcgcgcgg ggtgttcatc
     3001 ctcgccttcc tggtgctggt gatcggcggc tcgctcgccc tgtacgcggt gcgcgcgccg
     3061 cggctgcgcg ccggcggcct gttcgcgccg ctcagccgcg agggcctgct ggtgctcaac
     3121 aacctgttgt tcgcgaccgc cgccgcgacc gtgcttctcg gcacgctcta cccgctgttc
     3181 gtcgacgcgc tcggcgccgg caaggtgtcg gtcggcccgc cctacttcaa cgccgtgttc
     3241 atcccgctga tggcgccgct gatcgcgctc gcggggctga cgccgctgat gccgtggaag
     3301 cgcggcgacc tcgccggcgt gctcggccgg ctcaaggccg cggtcgccgc cgcgggcctg
     3361 gccgcgctcg ccgtcctcgt gctcgccgac cgcggcgggc tcttggccgc gctcggcatc
     3421 gcgcttgcgg cgtggcttgc ggcctcggtc gccgtcgaat gggcatcgcg cgtccgcctg
     3481 ttgcgcgccc cgctcgccga gtcgctcggc cgggcgcgcg ccttgccgcg cgccgcctac
     3541 ggcatgagcc tcgcccatct cggcgccgcg atcatggtcg ccggcatcac cgcgtcctcg
     3601 tcgtggcagg tcgagagctt ccaggtgatg cgcccgggcg agtcggtcac ggtggccggc
     3661 tacgactggc gcttccatgg tgtcgaggcc ctggacggcc ccaactattc gatcacgcgc
     3721 gcgcgcctga cggtcagccg cgacggccgc gaggtcgccg agatgctgcc cgagaagcgc
     3781 ttctatccgg tcgcgcgcat gccgaccacc gaggccgcga tccacaccac ctggctcgcc
     3841 gacctgtacg cggtgatcgg cgacggcgac ggcgagggcg gcttcagcac gcgcctctac
     3901 tacaacccgc tggtgccgtg gatctggctc ggcgcgatcg tgatggcgct gggcggggcg
     3961 acctcgctca ccgaccgccg ccaccgggtc ggcgtgccgc ggcgggcgca ggcgcacgcg
     4021 gccggcgcgc gcgcctaagg cgatggcggc gcggcactgg ctctacgtcg tcccggcggc
     4081 ggtgttcgcg actcttgcgg gcctgctcta ctggggcttg ttccaagacc cgagccaggt
     4141 gccgacggtc atggcgggcc gcacattgcc ggccttcgac ctgcctccgg tcgacggcgc
     4201 aaccccgggc ctagccagca gcgacgtcaa gaccggcgag atcagcgtga tcaacgtgtt
     4261 cgcctcgtgg tgcgtgccgt gccgcgccga gcacccgcag ctcaccgaga tcgccgcgct
     4321 cgagggggtg catctctacg gcatcgacta caaggacacg cccgaggacg tgcgcgcgtt
     4381 cctcgacgac ctcggcgatc cctatgcgcg catcggcgcg gatgcggacg ggcgggtcgg
     4441 catcgacctc ggcgtctacg gcgttcccga gacctacttc gtcggccgcg acggcctgat
     4501 ccactacaag catatcggcc cgatcgcgcc ggagcacatg accaagcgca tccggccgct
     4561 gatcgagcgc ctgaagcaat gactcgcgtc gcgcggcgtc tcgccggcat ggtccttgcc
     4621 gcgctcatgg ttggcgtggc ggcgacggcg gcgctcgcct tccagaccga cacgccgctt
     4681 gccgatcctg cgcaggaggc gcgcgcggtg gcgctggcca aggagctgcg ctgcctggtc
     4741 tgccagaacc agtcgatcat ggaatcggac gcggatctgg ccaaggacct gcgcggcatc
     4801 gtgcgcgagc gcattgccgc cggcgattcc gacgcgcagg tcaagcagta cgtggtcgag
     4861 cgctacggcg acttcgtgct gctgaggccg ccgttcaagg ccaagacctg ggcgctgtgg
     4921 ctcggcccgg cgctgatcgc ggcggcggca ctcgcggcgc tcgccgcggt ctggcgccgg
     4981 cgcgcgtcgc gggcccaggg cccggcgccg ctcaccgagg ccgagcgggc gcggctgcgt
     5041 gatttgcttg gggatgacgc gtgagtgcag ccgcgtgggc gctgttcggc gcgttgctgg
     5101 tcgccgcagt cgcggtgctc gcgcgcgccg cgctcgccgg cgcacgccgc ggcggcggcg
     5161 aggcgcgcgc cgcctacgac ctgcagatct accgcgacca gctcgccgag ctcgacaacg
     5221 atctcgaccg cggcgtggtc agtcccgagc aggcacaggc cgcgcgtacc gagatcgagc
     5281 gccgggtact ggcgctcggc acggcgccgg accgggggcc ggcgcgggcg gggcggcgca
     5341 cgggcggggt cacggcgggc gcgctggcga tcgcggtgcc ggcgctcacg gccctgatct
     5401 acgcgctggt gggaacgccc ggcgcgcccg acatgccgct ggctgcgcgc gaaagcgtgg
     5461 ccccgcccga gatgccggcc gacgtcgagg aggcggtcgg gcgcctggcg gcgcggctcg
     5521 agtccgagcc cgacgacctg caaggctggc tgctgctggg gcgctcctac gccttcctcg
     5581 agcgctacga ccaggcggcg gtcgcgttcg gcaccgccgc cgcgctcgtg cccgacgaca
     5641 ccgaggtcgc cgccgcctac ggcgaggcgc tgctctttgc cgccgacggg gtggtgacgc
     5701 cggctgcgcg ggagcagttc gaggccgtgc tcgcgcgcga tgccaagcat gagggcgcgc
     5761 ggctctatct cggcatggcg gcggcgcagg cgggcgagct cgagcgcgcg ttcgacatct
     5821 ggcgcgcgct cgccgaggac gccgacggct cggagccgtg gctcgccgcg ctgcgcgcgc
     5881 agctgatggc actcagccgc gagctcggcg tcgaggcccc ggcggtggcg ctggaggcgg
     5941 cgccgaccgc gccggcagcg gaggctccgg ggcccgggcc cgaggacctc gagatggcgg
     6001 ccgggatgtc gccggacgag cgcatggcgg cgatcgacag catggtcgcg cggctcgaag
     6061 ggcggctgat cgagcatccg gacgacccgg acggctggaa gcggctcggg cgcgcgaagc
     6121 gcgttctcgg cgacctcgcc ggcgcgcgcg cggcctacgg cgaggcggtc aagcgcgcgc
     6181 ccgcggacgg cgcggcactc gccggtctcg ccatggtcat ggtcgagcag acggggcccg
     6241 agaccgtgac cccggaggcg gcggacctgt tccggcgcgc gctcgccctc gagcccgcca
     6301 acggcgaggc gctctggttc ctcggcctcc tcgccgccca gagcggcgat gccgaccagg
     6361 cggtggcgat gtggcgccgc ctgctcggcc agctcgcgcc cggcagcgac gagcaccgca
     6421 tgctcagccg gcgcatcgac gagctggcgg cgaaggagtg agcggcgcac gtgtccgcgc
     6481 cgcctcggat tggattggcg cgtgccgccg ggtgcgctag tttttcgccg acaggatcac
     6541 ggcggccggc accgcctgcg gccgcagaaa ggattccgcc atgagcgtcg cacgcgccgc
     6601 cgacccggcc gaccccggcc gcgatttcgc cgacttctgg aaccgggtcc tggcgccgaa
     6661 gttcatccgc tacaagcaca tcatggtcga cggcctgacc catcacagcg aggcgatctt
     6721 cccgtcgctg ccggtcaggc agggcgacgc ggtgctcgac gtcggctgcg gcttcgccga
     6781 caccgcgatc cggctggcga gaacggtcgg cgcgcgcggc cgagtcgccg gcatcgattg
     6841 ctgcgcggcg tttctcgacg tcggccgcgc cgacgtcgcc gccgccgggc tgtcgaacgt
     6901 caccctcatc ctgggcgacg cccagagcta tccgttcgag ccggagtacg atttcgtgtt
     6961 ctcgcgcttc ggcacgatgt tcttcgagaa ccccgtgtgg gggctgagga acatgcgcac
     7021 cgcgctcaag cccggcgcgg ccatgaccat gattgtctgg cgaacgatcg acgacaatcc
     7081 ctggctcggc ctgcccaaga aggtcgtgct cgacttcctg ccgccgccgg gcgagagcgc
     7141 cgagacctgc ggccccgggc cgttctcgat ggccaaccgg gacacggtca ccggcatgct
     7201 caaggccgcc gggtacctgg agcccgcctt caagcgcgtc gacgccccgg tgcgggtcgg
     7261 caagacggtc aaggactcga cggatttcca gctcgcgctc ggcccggcgg gcgaggtgtt
     7321 ccgcgaggcg ggcgagctgg cggagcggcg ccgtgccgag atcgaggccg cgctcggcga
     7381 ggcgatcgcg ccctaccaga ccgacgaagg cgtggtgatg cagtcgagct cctgggtggt
     7441 caccgcgcgc aatcccggat agcggccccc gcgccgcccg cccgcccgcg cgcgtcggcg
     7501 cgggtgcaac tcaatcggtc ggcgggcgcg cgccgagcct gtcgatcgcg gcccataccc
     7561 ggcgcagggt cgccgggtcc ttgtacggca cgcgttcggg gtcgatgccg cccttggggc
     7621 tcaacctctg cacctcggcg agctcggcgc gcccgtcctc gtcgcggccg agctccgagt
     7681 aggccgcggc caggatctgg tgcgccgcgg ggaaatccgg gttgcgctcg agcgagcgct
     7741 tgaggaactc gaccgcctgc tggtggcggc cggcgaggaa atgcgcgtgg ccgacttgca
     7801 tcagcaggtc gaagcggacg ttggggtcga gccgcatcgc cttctcggcc atcgcgatcg
     7861 cgttctcggg ctcgcccgcg aacacgtaga tgcctgaaag catgatgtag gcgtcggcgt
     7921 agttgggatc gagcgcgatc gccttgagcc cggcggcgat ggcgacatcg tgctggcgct
     7981 gccaggactt gacccaggcg agccgggcaa gggcgagcgg caaatcgggg tcgagcgcga
     8041 ccgccttctc ggccgccgcc gcggccttgt tcagcgtgtc ggcgtcgtcg gtccaggaat
     8101 aggcccagtc gcggaaaaag acgtaggcga gctcggcata ggcgccggcg aatccgggat
     8161 cgagcgcgat cgccttgtca agcatctcct cggccaggcg attggtctcg cgcgtggtgc
     8221 ggtccttgta ggcgcgcccg cgcaggaagt agtcgtaggc ctcgacgttg tcggtcagct
     8281 tgcgggtggc cgccggggac gccggcccgg ccagctcgag ctcgagcgcg gcgacgatct
     8341 ggcgggtaat gtcgtcctgg accgcgaaga tgtcggtcaa ctcgcggtcg tagcgctccg
     8401 cccagacatg gccgccggac tccgcgtcga tcagctgggc ggtgacccgc acctgctcgc
     8461 cggcgcgccg cacgctgccc tcgaccacgt agcgcacgcc gaaggcgcgc gccacctcgg
     8521 gcacgtcgac cgaccggccc ttgtactgga aggtcgagtt gcgcgcgatc acgaacaggc
     8581 cgtggatgcg cgacagctcg gtgatgatgt cctcggtcag cccgtcgctg aagtactcct
     8641 gctcggcctc ctcgctcatg ttggtgaacg gcagcacggc gatcgacggc ccgtcgggca
     8701 cggtcagggc gagcgcgctc gcagccgccg gcaccgtgtc cgggcctgca ttgcgcccgg
     8761 ccgtcggcgt cgggcgctcg atcgcccaca gcgccgccac cgccgccacc accgcgacca
     8821 gcgccgccgc gccggcccgg agccggcggc gtcccctcgc cgccgacgcg ggccgcccgc
     8881 cggtgccatc cgcggcgtcg gtcacccgcc aggcgcgcac cggctcggcg atgttcttga
     8941 ccttgtgggt gccgccatcg aggaagccga catcgagctt gccgcgcacc tgcatgtgcg
     9001 ccgattccga gaccaccacc gtccccggct cggcgagagc ctcaaggcgc gcggcgatgt
     9061 tgacgccgtc gccgtagatg gtgccgtcgt cttgggcgat gacgtcgccc aggttgacgc
     9121 cgatgcggaa cagcatgcgc cggtccgccg gcagcggcgc gttgctctcg gcctgcgccg
     9181 cctgcacctc gagcgcgcac tcgaccgcct cgaccacgct cgcgaactcg gccagcacgc
     9241 tgtcgccggc agtgtcgacg acccgcccgc cgcgcgccgc gatgcgctcg cggaagcgtg
     9301 cccgcgcctg gttcagcgcg cgcacggtcg ccgcctcgtc cgaggccatc agtcggctgt
     9361 agccggcgac gtccgcactg aggatcgcgg ccagcttgcg cttggtgccg gtgtccgcca
     9421 tcgcgacgat gtccatcgtc aacctgcccg aaagtctaga ccaagccgtg gcggggcgga
     9481 aattggggga ttatccgctt gacgtactat cgcgccttgc aatagggtga gttcgcatga
     9541 caggccagag agttaccgat gacattgaga tcactccaga aatgatcgaa gcaggagcct
     9601 cgattctttg ccatttccag acggagacgg caggtgaaga ttactgggca gaagaagtgt
     9661 acaaagcaat ggcctctcgg aaaccgcata gatcaaaccc atggtttccc cacctttttg
     9721 cgaagttcct catagctaac aagttgtgtt gaatacgcta tgtgcttaca tctatcttgc
     9781 attagataat ttaggcaaag ttgaagatta acaataaatg tttcgcgatt gttctttggt
     9841 gttttaattt ttttgcttgt aggagtgccg aatcgtgcat ctggataagt ttcatgtcgt
     9901 tcgtggagat ttagtttagg caaatgtttg gtaaatttag gatcatcgga atcgaaatta
     9961 acacccatgt tgtgggcaaa gaaattgcga attctacata tacccagcat tgtttcgcga
    10021 acatggcttt cgattcccca aagcatatag agaacatcaa tttgtgctga cgtgctggaa
    10081 aggggcttgg ttagatcaaa gagacggtcc gagacatctt tgttgtcgcg cattcgttcc
    10141 ttaagtgttc gctccacggc ttcttcgagg aatgttccgc cgatgatagc gacgacaata
    10201 tcatcggctg acgacatgag atgcgttata gcttcgaatt tgacatcatg atctccaaag
    10261 ataggccaaa ccatggtaga ctctccatat ggcaaacaag cgatcaattg gcacagccaa
    10321 agaagcgtca gcggaacaac gcgatacgct gctactcaag ctcttgcaaa cggcacccca
    10381 gcctcgacca aaacgaaaac gggatcataa gaatgaggga ccggacacgg agcccggccc
    10441 ctcggggtct acgtcaacaa tgtcacgatc acccaagcga tcgcccagac gatcgcggcc
    10501 acgttgacgt gtaccctgac aaccagaatg gtcgtcttca taacaagacg gcctttctgg
    10561 cccacggcgc cgcgaaccaa aaaggttggc ggtatcgcgc ccgtcgaaca agcttaaccg
    10621 ctgttcacag gaagcatcgg cccactcttc acgttgtaca cccatgtcga gcggtcccac
    10681 ggtcggtagc ccggccatta ctggatgggg cctcgtgggt cacggagtat cccggccaga
    10741 gtagccgcgc cgattcgcgg ggctggttgg ggtccgcttg tagagggtgg gccgattctc
    10801 tactcagagt cgagttgatt tttgcgccag cgcaagaagg ttcgcgcctt ctgtttaggc
    10861 gtgcgcggct tcgtcagtcc gccgataggt
//
LOCUS       DASWHU010000035         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3336558, whole genome shotgun sequence.
ACCESSION   DASWHU010000035 DASWHU010000000
VERSION     DASWHU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3336558"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGB88_01225"
     CDS             <1..724
                     /locus_tag="VGB88_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9646087.1"
                     /translation="PVAFGQRIAHLANHFIWYGTRKPFATLTQSFRETADGAFNAHHY
                     RYAPDMSTFLVECEAATFARAGFARASEADIKGYCERLFADELDGHELLSNRSVWRNF
                     PQITNDRWFAGNRVLVGDALHTAHFSIGSGTRLALEDAIALAGALRAHDYDIQPALAA
                     YQAARRPVVDKMVRAADASATWYQDFATHMKHEPWRFAYSYIQRSGRIDGARLRRIAP
                     AFARELARRGILDEADNVESTR"
     gene            721..>1575
                     /gene="dusA"
                     /locus_tag="VGB88_01230"
     CDS             721..>1575
                     /gene="dusA"
                     /locus_tag="VGB88_01230"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX9646088.1"
                     /translation="MIDRRLSVAPMMDRTDRHGRFFLRQLSRRVLLYTEMVPAAALVH
                     GDAERWLAFDASERPLALQVGGGDPGPLAACARLAHARGFDEINLNVGCPSERVRRGC
                     FGAALMAEPERVAACVAAMRAAAPLAVTVKTRIGIDDHDDYGFLAGFVATVAAAGCAA
                     FIVHARKAVLGGLSPRRNREVPPLRYDRVWQLKRDFPALEIVVNGGVRTLEAVHEQLC
                     HVDGVMIGRAAYDDPYLLADADRAIFGAPAHAPDRHQVVDRMLPYIAREQGGAVPLQA
                     ITRHMLGLF"
BASE COUNT          219 a          572 c          549 g          235 t
ORIGIN      
        1 cccggtcgcg ttcgggcaac ggatcgcgca cttggctaac catttcatct ggtacgggac
       61 gcgcaagccg ttcgcgacgc tcacccagtc gttccgcgag actgccgacg gggcgttcaa
      121 cgcccatcat taccgctacg cgcccgacat gagcacgttc ctggtcgagt gcgaggcggc
      181 cacgttcgcc cgcgccggct tcgcgcgcgc gagcgaggcc gacatcaagg gctattgcga
      241 gcggctgttc gcggacgagc tcgacggtca cgagctgctg tcgaacaggt cggtctggag
      301 aaacttcccg caaataacca acgatcgctg gttcgccggc aaccgggtgc tggtcggcga
      361 cgcgctgcac accgcgcatt tctcgatcgg ctcgggcacg cggctcgcgc tggaagacgc
      421 gatcgcgctg gccggcgcgc tcagggcgca cgattatgac atccagccgg cgctcgcggc
      481 ctatcaggcg gcgcgccggc ccgtcgtcga caagatggtg cgcgccgccg acgccagcgc
      541 cacttggtac caggacttcg ccacccatat gaagcacgag ccatggcgct tcgcctacag
      601 ctacatccag cgctccgggc gcatcgacgg cgcccgcctg cgtcggatcg cgccggcctt
      661 cgcgcgcgag ctcgcgcggc gcggcatact ggacgaggct gacaacgtgg agtccacgcg
      721 atgatcgacc gcaggctttc ggtcgcgccg atgatggacc ggaccgaccg gcacggccgg
      781 ttcttcctgc gccagctctc gcgccgcgtg ctgctctata ccgagatggt gccggcggcg
      841 gccctggtgc acggcgacgc cgagcgctgg ctcgcgttcg acgccagcga gcggccgctc
      901 gcgctgcagg tgggcggcgg cgatcccggg ccgctcgcgg cttgcgcgcg gcttgcgcat
      961 gcgcgcggct tcgacgagat caacctcaat gtcggctgcc cgagcgagcg ggtgcggcgc
     1021 ggctgcttcg gcgccgcctt gatggccgag cccgagcggg tcgcggcgtg cgtcgccgcg
     1081 atgcgcgccg ccgcgccgct ggcggtcacg gtcaagaccc ggatcggcat cgacgaccac
     1141 gacgactacg gctttctcgc cggcttcgtg gcgacggtcg cggcggccgg ctgcgccgcc
     1201 ttcatcgtgc acgcgcgcaa ggcggtgctc ggcgggctca gcccgcgccg gaaccgcgag
     1261 gttccgccgc tgcgctacga ccgcgtctgg caactcaagc gcgacttccc ggcactcgag
     1321 atcgtggtca acggcggcgt gcgcacgctg gaggcggtgc acgaacagct ttgccatgtc
     1381 gacggcgtga tgatcggccg cgcggcctac gacgacccgt atctgctggc cgacgccgac
     1441 cgggcgatct tcggcgcccc ggcgcacgcc ccggaccgcc accaggtggt cgaccggatg
     1501 ctgccctaca ttgcgcgcga gcaaggcggc gcggtgccgc tccaggcgat cacccgccac
     1561 atgctcggcc tgttc
//
LOCUS       DASWHU010000036        10358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2968899, whole genome shotgun sequence.
ACCESSION   DASWHU010000036 DASWHU010000000
VERSION     DASWHU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968899"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /gene="purH"
                     /locus_tag="VGB88_01235"
     CDS             complement(<1..1473)
                     /gene="purH"
                     /locus_tag="VGB88_01235"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9646089.1"
                     /translation="MSDDLRAIGRALLSVSDKTGLAAFAGALSARGAALVSTGGSARA
                     LREAGLAVTEVAELTGQPEMMGGRVKTLHPLVHGGILARRDLEADCAAMAAHGICAID
                     LVAVNLYPFERAVAGGADEATCIETIDIGGPALIRSAAKNHAYVTVVTDPADYAAVID
                     ELARHDGKVGGALRRELAARAFARTAAYDAAIAQWFAGQATGGLPARLTVSMPRRQGL
                     RYGENPHQRAAFYVDTSPRPGVATATQLQGKELSYNNIADTDAAYELVAELERPAAAI
                     VKHANPCGVAVGKTSLDAYRRALACDPLSAFGGIVAVNRALDAALARAVCELFTEVVI
                     APEADAEARAVFAERKNLRLLVAGALPDPQAPALALRSVAGGVLVQDRDARRIGPADL
                     KVVTRRAPDEAEIADLTFAFTVAKHVKSNAIVYAKGGATVGIGAGQMSRVDAVRLAVA
                     KAGDAARAAGESGRRTDGAAVASDAFFPFTDGLIAAAEAGA"
     gene            complement(1470..3413)
                     /locus_tag="VGB88_01240"
     CDS             complement(1470..3413)
                     /locus_tag="VGB88_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX9646090.1"
                     /translation="MDIRLHRIKGSRAWLALGHDTTMAAVSFVGALWLRLGDDFLPQT
                     AGFLVSATVLFTAIAAATFVGMGLYRGVWRYASINDLIAIARAVTVTVLLFLTAMFLL
                     TRLELMPRSALAIDWLLLLALLGGPRFAYRLAKDGNLIGIAESGYDARIPVLLVGAGE
                     SGEAFLRAMRRVGARYRVVGILDDDPRRAERRIHGVDVLGPSSGLVEAVARLLRAGQR
                     PRRILIGDERLAGAAIGALLEQADALGMTLARVPRATDFRPGAEAGNGGNGIEVRPVA
                     LEDLLGRAQARLDQRAMSALIAGRPVLVTGAGGTIGAELVRQIARRAPALLVLLDAGE
                     HNLYTIDREVAGQWPELAREAVLADVRDAAAIAAAFARARPELVFHAAALKHVPIAEA
                     HASETTLTNVLGTRIVADAARAHGARAMVLISTDKAVNPAGVMGATKRLAESYCQALD
                     LAAGAGETRFLTVRFGNVLGSTGSVVPLFQSQLAAGGPLTVTHPDMSRYFMTAREAVE
                     LVLQASAMGLDDDAAAPAGGIYVLDMGEPVRIVDLARQMIRLAGLHPDADVRIAFTGP
                     RPGEKLHEQLFHPGEHAVATAHDAIRLARPRAADAETLARAISAIGEVAARHDDAAVR
                     QRLAQLVPDYAGSEPGEARAAAR"
     gene            complement(3434..4447)
                     /locus_tag="VGB88_01245"
     CDS             complement(3434..4447)
                     /locus_tag="VGB88_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9646091.1"
                     /translation="MQAVTPAAVALACAACAWAATGLVLRLLLRAAVLDHPNPRSSHD
                     RPTPRGGGIAVVAVTLAAWALLAAFGAVAWTLVVPIAGLAVGLAMVSWADDLRGLGPL
                     PRFAAQLAAIGVALATVPLGPQFPGAWPAGLAFAAAALAWLWFVNLYNFMDGIDGLAG
                     AETVAVAAGVALVAGLAGMADGTPLLAAALAGAAAGFLVWNWQPARLFLGDVGSVPMG
                     FLLGWLLLSLAAHGDWAAALILPGYFLADASLTLARRALRGEAVWRAHRDHVYQRAVR
                     AGASHGRIAGTVSAANLALVACALASRAGPNAAWPALAGAGLIVALLLWYLGRGAPAA
                     WAR"
     gene            complement(4435..5802)
                     /locus_tag="VGB88_01250"
     CDS             complement(4435..5802)
                     /locus_tag="VGB88_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9646092.1"
                     /translation="MAQAEGAVGPAHPGRVNWRALAVVAGLALVVRLAAVVALDALSG
                     ADAFLIADSAAFLHAAHGARELQFMPLYPAFLAAHAALLGPDPLWPVLSQAVVDSATC
                     VVVARIAAELAPALELPAGVFAALNPTQVVMATLVLTETLFVFFAALALWAALAWLRR
                     PGWRNAAGLGLVLGLGALTRVMLLPWAVGGTVLLVVLAAALRRPLRRAIAQAALALGL
                     ALVQLGPVLAANYARTGGWGLTNQGGGHALYWVVPLVIEARDGTPHADGARRMAERFE
                     PAVRAAPDPFSRSRALGRAALAMLAELGPGPVVEAWAIGAAINLAAPAVILATPVRAL
                     PRTGFYATPGAAKLDKVWAFLFENDNPLYGWILVVSAAGTIACRAAQGFGLWRGVRAA
                     LAGNRALGAALVLLLAWIGFILLVNGPVASAKYRAPIEPALAALAALAVRRPGAAAIT
                     APCKP"
     gene            complement(5778..7250)
                     /locus_tag="VGB88_01255"
     CDS             complement(5778..7250)
                     /locus_tag="VGB88_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HEX9646093.1"
                     /translation="MADAAMTESGAGRALTPRKRAIIELADRVAPERRRWIARNRYFY
                     DDHYHYMRFLVGENARVLDLGCGTGELLAALAPRRGVGVDISPRMIEIAQQQFPDYRF
                     VLGDIEDTHVLDELDGPFDVIVLSEAIGSLEDIETTLRALHRLCTRDTRLIVAYYSRA
                     WQPVLTLAEMLGSKQRQLPLNWLSTDDIASLLGLADFEMIKREWRQLLPKRLLGLGPL
                     VNRSLGTLPGLRRFSLRSYVIARPRAGLWPWPSAPSATVLIPCRNERGNIEPAIRRMP
                     RFAPEQEILFVEGHSADGTFEEIERAAAAHPEWTIRALRQPGRGKGDAVRTGFAAARG
                     EVLMILDADLTVEPEALPKFYDALVAGKGEFINGTRLVYPTERQAMRPLNLIANRAFS
                     LLFSWLLNQRLTDTLCGTKVLRRAHYDRIAANRGYFGDFDPFGDFDLIFGAAKLNLKL
                     VEIPIRYQARAYGETQISRFADGWLLLKMVLFAWRKLKAL"
     gene            complement(7254..8450)
                     /locus_tag="VGB88_01260"
     CDS             complement(7254..8450)
                     /locus_tag="VGB88_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX9646094.1"
                     /translation="MAVAAELRPRLAMYAIALRRKLAILLTGDFDPYPPPPAGISLSP
                     YERDRAEAEAAPCALSWANSGGDVRAWQDAARAKLAGLAGWRRQGALPARSRSGFASA
                     EAGPPEAIVRDELAPARHGLNHVRSYLRVRASADVPVHVLWHPAQRWPAPAMICLQGT
                     NAGAHLGWGEARMPPDPVKIAAGLDFALQAAARGFVALCIEQRCFGERRERILAPRSA
                     DPCVDAVMHALLLGRTLIGDSAGDVSAVIDWVDAGGPSLDLDRARIYALGHSWGGTVA
                     LYAAALDTRIAGVIASGCVGPIRDTVAARRAPSGQNVIPGLLDWLELADVLALCAPRP
                     LLAVSGRADHIFPFKGAERAVAEALPVYRALDAGRRLRALALPGGHRFAPEAIWPAFE
                     SLIADG"
     gene            complement(8450..9460)
                     /locus_tag="VGB88_01265"
     CDS             complement(8450..9460)
                     /locus_tag="VGB88_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646095.1"
                     /translation="MADYAGVRVLVTGADGFIGSHLVERLAAGGAKVTALALYNAFDS
                     HGWLDELPAPMRASVRIERGDVRDAALVLRLCRDQEVVFHLAALIAVPYSYGAAQSYI
                     DVNVTGAVNLLEAARAHAPRRVVMTSTSEVYGTARRTPIDEDHPLRGQSPYAASKIAA
                     DAIAEAYALSFALPVVVLRPFNTYGPRQSERAVIAAAIRQALDPDCAEIRLGDLSPSR
                     DFTFIADTVAAFAAAGSAEGLAYGVPYNAGTGVALSIEQVVEVIRRLVGTNKPVVEEA
                     VRRRPAGSEVLALLADSTRFSAAAGWRAETGLDQGLALTVEWWRERLARGAVRAASGF
                     IT"
     gene            complement(9546..>10358)
                     /locus_tag="VGB88_01270"
     CDS             complement(9546..>10358)
                     /locus_tag="VGB88_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9646096.1"
                     /translation="PAAEALLEAAWQGGIRWFDTARAYGEAEARIGRWLRARGRAPHL
                     VTKLAPVESAAGVAAGLDAATAALGRRPDIVLAHRGSDITRPAVAGALAEAVAAGRIS
                     AFGASVYSPDEARRALAVAGIGALQLPLSLADRRALDEGVIDQACAQGVRLFARSVFL
                     QGALVMPAAALPEHLAALRAPLSRLARIADEAGCTVAALALAGVLTVAAVERVVIGAE
                     RPEQIAADLAAAARPPAPDAVEAAIAALAALPLAVLEPPRWRQGAPAARGFG"
BASE COUNT         1441 a         3876 c         3880 g         1161 t
ORIGIN      
        1 cgcgcccgcc tcggcggcgg cgatcaggcc gtccgtgaac gggaagaacg cgtccgaggc
       61 gaccgcagcg ccgtcggtgc gccggccgct ctcgcccgcc gcgcgcgcgg catcgcccgc
      121 cttggcgacg gcgaggcgga ccgcatccac ccggctcatc tgccccgcgc cgatgcccac
      181 ggtcgcgccg cccttggcgt agacgatggc gttcgacttg acgtgcttgg cgaccgtgaa
      241 cgcgaaggtg aggtcggcga tctcggcctc atccggcgcg cgccgggtga cgaccttgag
      301 gtcggcgggc ccgatccgcc gcgcgtcgcg gtcctggacc agcacgccgc ccgcgaccga
      361 gcgcagcgcg agcgcgggcg cctgcgggtc gggcagcgcg cccgcgacca gcaggcgcag
      421 gttcttgcgc tcggcgaaca ccgcgcgcgc ctcggcatcc gcctcaggcg caatcaccac
      481 ctcggtgaac agctcacaga ccgcgcgcgc gagcgccgca tcgagcgcgc ggttgaccgc
      541 gacgatgccg ccgaaggcgc tcaacggatc gcaggcgagc gcgcgccggt aggcatcgag
      601 cgaggtcttg ccgaccgcca ccccgcacgg gttggcgtgc ttgacgatcg ccgccgccgg
      661 ccgctcgagc tcggccacca gctcgtaggc cgcatcggtg tcggcgatgt tgttgtagct
      721 cagctccttg ccctggagct gggtggcggt ggcgacaccc gggcgcgggc tggtgtcgac
      781 atagaacgcc gcccgctggt gcgggttctc gccatagcgc aagccctggc ggcgcggcat
      841 cgagacggtg aggcgcgcgg gcagcccgcc ggttgcttgg ccggcgaacc attgcgcaat
      901 ggcggcatcg tacgccgcgg tgcgggcgaa ggcgcgcgcg gcgagctcgc ggcgcagcgc
      961 gccgcccacc ttgccgtcgt ggcgggcgag ctcgtcgatc accgcggcgt agtcggcggg
     1021 atcggtgacc acggtgacgt aggcgtggtt cttggccgcc gagcggatca gcgccgggcc
     1081 gccgatgtcg atggtctcga tgcaagtcgc ctcgtcggcg ccgccggcga cggcgcgctc
     1141 gaacgggtac aggttgaccg ccaccaggtc gatcgcgcag atgccgtgcg cggccatcgc
     1201 ggcgcagtcg gcctcgaggt cgcggcgcgc caggatgccg ccgtggacca gcgggtgcag
     1261 ggtcttgacc cgcccgccca tcatctcggg ctggccggtg agctcggcga cctcggtgac
     1321 cgcgaggccc gcctcgcgca acgcgcgcgc cgagccgccg gtggagacga gcgccgcgcc
     1381 gcgcgcgctc agcgcgccgg cgaaggcggc cagcccggtc ttgtccgaga ccgacagcag
     1441 cgcgcgcccg atagccctca ggtcgtcact caccgggcgg cggcccgcgc ctcgccgggc
     1501 tcgctgccgg catagtctgg caccagctgg gccaggcgct ggcgcacggc ggcgtcgtcg
     1561 tggcgggcgg cgacctcgcc gatggcggaa atcgcgcgcg ccagcgtctc ggcgtcggcc
     1621 gcgcgcgggc gggcgagccg gatggcgtcg tgcgcggtcg ccaccgcgtg ctcgcccggg
     1681 tggaacagct gctcgtgcag cttctcgccc gggcgcgggc cggtgaaggc gatgcgcacg
     1741 tcggcatcgg ggtgcaggcc ggcgaggcgg atcatctggc gggcgaggtc gacgatgcgc
     1801 accggctcgc ccatgtccag cacgtagatg cccccggccg gcgcagcggc gtcgtcgtcg
     1861 aggcccatcg ccgaggcctg gagcaccagc tccaccgcct cgcgcgcggt catgaagtag
     1921 cggctcatgt cggggtgggt gacggtcagc ggcccgccgg cggcgagctg gctctggaac
     1981 agcggcacca ccgagcccgt cgagcccagc acgttgccga agcgcacggt caggaaccgg
     2041 gtctcgcccg cgcctgcggc gaggtcgagc gcctggcagt agctctcggc gaggcgcttg
     2101 gtcgcgccca tgactccggc cgggttgacc gccttgtcgg tcgagatcag taccattgcg
     2161 cgcgcgccgt gggcgcgggc cgcgtcggcg acgatgcggg tgccgagcac gttggtcagc
     2221 gtcgtctcgc tggcatgcgc ctcggcgatc ggcacgtgct tcagcgccgc ggcgtggaac
     2281 accagctccg ggcgcgcgcg ggcgaaggcg gcggcgatcg cggccgcgtc gcggacgtcg
     2341 gccagcaccg cctcgcgcgc gagctccggc cactgcccgg cgacctcgcg gtcgatggtg
     2401 tagaggttgt gctcgccggc atcgagcagc accagcagcg ccggcgcgcg gcgcgcgatc
     2461 tggcgcacca gctccgcccc gatggtgccg ccggcgccgg tcaccagcac cgggcggcct
     2521 gcgatcagcg cgctcatcgc ccgctggtcg aggcgcgcct gggcgcggcc gagcaggtcc
     2581 tcgagcgcca ccggccggac ctcgatgccg ttgccgccgt tgccggcctc ggcgcccggg
     2641 cggaagtcgg tcgcccgcgg cacccgggcc aaggtcatgc cgagcgcgtc ggcctgctcg
     2701 agcagcgcgc cgatggcggc gccggcgagc cgctcgtcgc cgatcaggat gcggcgcggg
     2761 cgctggccgg cccgcagcag ccgcgccacc gcctcgacca ggcccgacga cggccccagc
     2821 acatcgacgc cgtggatgcg ccgctccgcc cggcgcgggt cgtcgtcgag gatgccgacc
     2881 acgcggtagc gggcgccgac cctgcgcatg gcgcgcagga acgcctcgcc cgactcgccg
     2941 gcgccgacca gcagcaccgg gatgcgcgcg tcgtagccgc tctcggcgat gccgatcagg
     3001 ttgccgtcct tggccagccg gtaggcgaag cgcggcccgc ccaacagcgc cagcagcagc
     3061 agccagtcga tcgccagcgc cgagcgcggc atcagctcga gccgggtgag caggaacatc
     3121 gcggtcagga acaggagcac cgtcaccgtg accgccctgg cgatcgcgat caggtcgttg
     3181 atcgaggcgt agcgccacac cccccggtac aggcccatgc cgacgaaggt ggcggcggcg
     3241 atggcggtga acagcaccgt cgccgagacc aggaagcccg cggtctgggg caggaaatca
     3301 tcgcccaggc gcagccacag cgcgccgacg aacgagaccg cggccatggt cgtgtcgtgg
     3361 cccagcgcca gccacgcccg gctgcccttg atccggtgca gtctgatgtc caagcccgcc
     3421 cccgccttcc cggtcagcgc gcccaggcgg cgggcgcgcc gcggccaagg taccacagca
     3481 gcagggcgac gatcagcccc gcgccggcca gcgccggcca cgcggcgttc gggccggcgc
     3541 ggcttgccag cgcgcaggcc accagcgcca ggttggcggc gctcaccgtg ccggcgatgc
     3601 ggccgtggct cgcgccggcg cggaccgcgc gctggtagac gtggtcgcgg tgggcgcgcc
     3661 acaccgcctc gccgcgcagc gccctgcggg caagggtgag gctcgcgtcg gcgaggaagt
     3721 agccgggcag gatcagcgcc gccgcccagt cgccgtgggc ggcgagcgac agcagcagcc
     3781 agccgagcag gaagcccatc ggcacgctgc cgacatcgcc caggaacagc cgcgccggct
     3841 gccagttcca gaccaggaac ccggccgccg cgccggccag cgccgcggcc agcagcggcg
     3901 tgccgtcggc catgccggcg aggcccgcga ccagggcgac gccggccgcc accgccaccg
     3961 tctcggcgcc cgccaggccg tcgatgccgt ccatgaagtt gtacaggttg acgaaccaca
     4021 gccacgccag cgccgccgcg gcgaaggcga ggccggcggg ccaggcgccg gggaactggg
     4081 ggccgagcgg cacggtggcc agcgccacgc cgatcgcggc gagctgggcg gcgaagcgcg
     4141 gcaggggccc gagcccgcgc aggtcgtcgg cccacgacac catggcgagg ccgacggcga
     4201 gcccggcgat cggcaccacc agggtccagg cgaccgcgcc gaaggcggcg agcagcgccc
     4261 acgcggcgag cgtcaccgcg accacggcga tgccgccgcc gcgcggggtc gggcggtcgt
     4321 ggctcgagcg cgggttgggg tgatccagca ccgccgcccg cagcaacagg cgcagcacca
     4381 gcccggtcgc cgcccacgca caggccgcgc aggccagcgc cacggccgcg ggagtcacgg
     4441 cttgcatggt gccgttatag ccgccgcgcc cggccggcgc accgccagcg cggccaatgc
     4501 cgcgagcgcg ggctcgatcg gcgcccggta cttggccgat gccaccgggc cgttgaccag
     4561 caggatgaac ccgatccagg cgagcagcag caccagcgcc gcgccgagcg cccgattgcc
     4621 ggccagcgcc gcgcgcacgc cccgccacag cccgaacccc tgggccgccc ggcaggcgat
     4681 ggtgccggcc gccgacacca ccaggatcca gccatagagc gggttgtcgt tctcgaacag
     4741 gaaggcccac accttgtcga gcttggcggc gcccggggtg gcgtagaagc cggtgcgcgg
     4801 cagcgcgcgg acgggcgtgg ccaggatcac cgccggcgcg gcgaggttga tcgcggcgcc
     4861 gatcgcccac gcctcgacca ccggccccgg gccgagctcg gcgagcatgg caagcgccgc
     4921 gcggccgagg gcgcgcgagc ggctgaacgg gtccggcgcc gccctgacgg cgggctcgaa
     4981 gcgctcggcc atgcgccgcg cgccgtccgc gtgcggggtg ccgtcccgcg cctcgatcac
     5041 cagcggcacc acccagtaca gcgcgtggcc gccgccctgg ttggtcaggc cccagccgcc
     5101 ggtgcgcgcg tagttggcgg ccagcaccgg gccgagctgg accagggcca gccccagtgc
     5161 cagcgccgcc tgggcgatcg cgcgccgaag cgggcggcgc agcgccgccg ccagcaccac
     5221 cagcagcacc gtcccgccga ccgcccaagg cagcagcatc acccgggtga gggcgccgag
     5281 gccgaggacg agcccgagac ccgccgcgtt ccgccagccc ggccgcctga gccaggcgag
     5341 cgcggcccac aacgcgagcg cggcgaagaa cacgaacagc gtctcggtga gcaccagggt
     5401 cgccatcacc acctgggtcg ggttgagcgc ggcgaacacg ccggccggca gctcgagcgc
     5461 cggcgcaagc tcggccgcga tgcgcgccac caccacgcag gtggcggagt cgaccactgc
     5521 ctggctcagc accggccaca gcgggtccgg gccgagcagc gccgcatgcg cggcgaggaa
     5581 cgccggatag agcggcatga actgcagctc gcgcgcgccg tgcgcggcgt gcaggaacgc
     5641 cgccgagtcg gcgatcagga acgcgtccgc gcccgacagc gcgtcgagcg ccaccaccgc
     5701 ggcgaggcgg accaccagcg ccaggcccgc gaccacggcg agcgcgcgcc agttcacccg
     5761 gccggggtgc gccgggccta cagcgccttc agcttgcgcc atgcgaacag caccatcttg
     5821 agcagcagcc agccgtcggc gaagcgcgag atctgggtct cgccataggc gcgcgcctgg
     5881 tagcggatcg ggatctcgac cagcttcagg ttcagcttgg cggcgccgaa gatcaggtcg
     5941 aagtcgccga acgggtcgaa gtcgccgaaa tagccgcggt tggcggcgat ccggtcgtaa
     6001 tgcgcccggc gcagcacctt ggtgccgcac agcgtgtcgg tcaggcgctg gttcagcagc
     6061 cacgagaaca acagcgagaa ggcgcggttg gcgatcaggt tcaagggccg catcgcctgc
     6121 cgctcggtcg gatagacgag cctggtgccg ttgatgaact cgcccttgcc cgcaaccagg
     6181 gcgtcgtaga acttgggcag cgcctcgggc tcgaccgtga ggtcggcatc gaggatcatc
     6241 agcacctcgc cgcgggcggc ggcgaagccg gtgcgcaccg catcgccctt gccgcggccc
     6301 ggctggcgaa gcgcgcggat ggtccactcg gggtgggcgg cggcggcgcg ctcgatctcc
     6361 tcgaaggtgc cgtccgcgga gtggccctcg acgaacagga tctcctgctc gggcgcgaac
     6421 cgcggcatgc gccggatcgc cggctcgata ttgccgcgct cgttgcggca cgggatcagc
     6481 accgtcgccg acggcgccga gggccacggc cacagccccg cgcgcggcct cgcgatcacg
     6541 tagctgcgca ggctgaagcg gcgcaggccc ggcaaggtgc cgaggctgcg gttgaccagc
     6601 ggcccgagcc ccaacagccg cttcggcaac agctggcgcc actcgcgctt gatcatctcg
     6661 aagtcggcaa ggccaaggag tgaggcgatg tcgtcggtgc tcagccagtt gagcggcagc
     6721 tggcgctgct tgctgcccag catctcggcc aaggtgagca ccggctgcca ggcgcgcgag
     6781 tagtaagcga cgatcaggcg ggtgtcgcgc gtgcacagcc gatggagcgc gcgcaaggtg
     6841 gtctcgatgt cctcgagcga gccgatcgcc tccgacagca cgatcacgtc gaacggtccg
     6901 tccagttcgt cgagcacgtg cgtgtcctcg atgtcgccga gcacgaaccg atagtcgggg
     6961 aactgttgtt gcgctatctc gatcatccgc gggctgatgt cgacgccgac tccgcgccgg
     7021 ggcgccagcg ccgccagcag ctcgccggtg ccgcagccga gatcgagcac gcgcgcgttc
     7081 tcgcccacca ggaagcgcat gtagtggtag tggtcgtcgt agaagtagcg gttgcgggcg
     7141 atccagcgcc ggcgctcggg cgcgacccgg tcggcgagct cgatgatcgc gcgcttgcgc
     7201 ggcgtcagcg cccgccccgc gccgctttcg gtcattgccg cgtcagccat tgctcagccg
     7261 tccgcgatga gggactcgaa cgccggccag atcgcctcgg gcgcgaaccg gtggccgccg
     7321 ggaagcgcga gcgcgcgcaa tcggcgcccg gcatcgagcg cccggtagac cggcagcgcc
     7381 tcggcgaccg cgcgctcggc gcccttgaag gggaagatgt gatcggcgcg gccggagacc
     7441 gcgagcagcg ggcgcggcgc gcacagcgcc agcacgtcgg cgagctcgag ccagtcgagc
     7501 aggccgggga tgacgttctg gccgctcggc gcgcggcgcg cggcgacggt gtcgcggatc
     7561 ggcccgacgc agcccgaggc gatgaccccg gcgatgcgcg tgtccagcgc cgccgcatac
     7621 agcgccaccg tgccgcccca ggaatggccg agcgcgtaga tgcgcgcacg gtcgagatcg
     7681 aggctggggc cgccggcatc gacccagtcg atcaccgccg agacgtcgcc ggcgctgtcg
     7741 ccgataagcg tgcggcccag cagcagcgcg tgcatgaccg catcgacgca cgggtccgcg
     7801 gagcggggcg cgagaatgcg ctcgcggcgc tcgccgaagc agcgctgctc gatgcacagc
     7861 gcgacgaagc cgcgcgcggc ggcctgcagc gcgaagtcga gcccggcggc gatcttgacc
     7921 gggtcgggcg gcatgcgcgc ctcgccccag cccagatgcg cgccggcatt ggtgccctga
     7981 aggcagatca tcgccggcgc cggccagcgc tgcgccggat gccacagcac gtgcaccggc
     8041 acgtcggcgc tcgcccggac gcgcaaatag ctgcgcacgt ggttgagccc gtggcgcgcg
     8101 ggcgcgagct cgtcgcgcac gatcgcctcc ggcggccctg cctccgccga agcgaagccg
     8161 cttcggcttc gcgcaggcag ggccccctgc cgccgccagc cggcgagccc ggcaagcttg
     8221 gcgcgcgccg catcctgcca ggcgcgcacg tcgccgccgc tattcgccca cgacagcgcg
     8281 cacggcgccg cctcggcctc cgcccggtcg cgctcatacg ggctcagcga gataccggcc
     8341 ggcggcggcg gataggggtc gaagtcgccg gtcaacagga tcgcgagctt gcgccggagc
     8401 gcgatcgcgt acatcgccag acgtggtcga agctccgccg ccaccgccat caggtgatga
     8461 agccgctcgc cgcccgcacc gcgccgcgcg ccagccgctc gcgccaccac tcgacggtga
     8521 gcgcgaggcc ttggtcgagc ccggtctcgg cccgccaccc ggccgccgcg ctgaagcggg
     8581 tggaatcggc gagcagcgca agcacctccg agccggccgg cctgcggcgc accgcctcct
     8641 cgaccaccgg cttgttggtg ccgaccagcc ggcggatgac ctcgaccacc tgctcgatcg
     8701 acagcgcaac gccggtgccg gcattgtacg gcacgccgta ggccaagccc tcggcgctgc
     8761 ccgcggccgc gaacgccgcc accgtgtcgg cgatgaaggt gaagtcgcgg ctcgggctca
     8821 ggtcgccgag cctgatctcc gcgcagtcgg ggtcgagcgc ctgtcggatc gcggccgcga
     8881 tcaccgcccg ctcgctctgg cgcgggccgt aggtattgaa cggccgtaac accaccaccg
     8941 gcagggcgaa gctcagcgca taggcctcgg cgatcgcgtc ggcggcgatc ttcgaggccg
     9001 cgtagggcga ctggccgcgc agcggatggt cctcgtcgat cggggtgcgc cgggcggtgc
     9061 cgtagacctc gctggtcgag gtcatcacca cccgcctggg cgcgtgcgcg cgcgccgcct
     9121 cgagcagatt gaccgcgccg gtgacgttga cgtcgatata gctctgggcc gcgccatagg
     9181 agtagggaac cgcgatcagc gcggcgaggt ggaacaccac ctcctggtcc cggcacaggc
     9241 gcaggaccag ggcggcgtcg cgcacgtcgc cgcgctcgat ccgcaccgag gcgcgcatcg
     9301 gcgccggcag ctcgtcgagc cagccgtggc tgtcgaaggc gttgtagagc gccagcgctg
     9361 tcacctttgc gcccccggcc gccagccgct cgaccagatg cgagccgatg aacccgtcgg
     9421 caccggttac cagcaccctg acgcccgcgt agtccgccat cccccgcctt cgcgctgcga
     9481 ttcggtgggc ccgcccgccg gagcttcagc gaaggcgggc atccaatagc gtccgcgtgc
     9541 gcgggtcaac cgaaaccccg cgccgccggc gcgccctggc gccagcgcgg cggctcgagc
     9601 accgcgagcg gaagtgcggc cagcgccgcg atcgccgcct cgaccgcatc gggagccggc
     9661 ggccgggcgg cggcggcgag gtcggcggcg atctgctcgg gtcgctcggc gccgatcacc
     9721 acccgctcga ccgcggcgac cgtaagcacg ccggccaagg ccagcgccgc cactgtgcat
     9781 ccggcctcgt cggcgatccg cgccaggcgc gacagcggcg cgcgcagcgc cgcgagatgc
     9841 tcgggcagcg ccgccgcggg catcaccagc gcgccttgca ggaagacgct gcgcgcgaac
     9901 aggcggacgc cctgcgcgca tgcctggtcg atgacgccct cgtcgagcgc gcgccggtcg
     9961 gcgaggctga gcggcagctg caacgcgccg atgccggcga cggcgagcgc gcggcgcgcc
    10021 tcgtccgggg aatagaccga ggcgccgaaa gcgctgatcc ggccggcggc gacagcctcg
    10081 gcgagcgcgc cggcgactgc gggccgggtg atgtcgctgc cgcggtgggc gagcacgatg
    10141 tcggggcggc ggccgagcgc ggcggtggcg gcatcgagcc cggcggcgac cccggcggcg
    10201 ctctcgaccg gggcgagctt ggtcaccaga tgcggcgcgc ggccgcgcgc gcgcagccag
    10261 cggccgatgc gcgcttccgc ctcgccatag gcgcgggcgg tgtcgaacca gcggatgccg
    10321 ccctgccagg ccgcctcgag cagcgcctcg gccgccgg
//
LOCUS       DASWHU010000037         5649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2970108, whole genome shotgun sequence.
ACCESSION   DASWHU010000037 DASWHU010000000
VERSION     DASWHU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970108"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VGB88_01275"
     CDS             <1..419
                     /locus_tag="VGB88_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX9646097.1"
                     /translation="VRIVLDTNVLIGALITKGTPPDRLYQAWLRGEIDLVTCTAQIVE
                     VADVLSRPRLLKYVDADEAAAIVENIDTRAVILDDPPDVDLSPDPKDNPILAAAIAGK
                     VDLIVSGDKKHMLAFGEVEGIPIVTARDALDRLSKE"
     gene            complement(523..2097)
                     /gene="serA"
                     /locus_tag="VGB88_01280"
     CDS             complement(523..2097)
                     /gene="serA"
                     /locus_tag="VGB88_01280"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9646098.1"
                     /translation="MAKVLIADSLSPSAADVFRERGIEVDVAPGLAADELIARIAGYD
                     GLAVRSATKVTAAVLDAGRRLKVVGRAGIGVDNIDVAAATARGVVVMNTPFGNSVTTA
                     EHTLAMIFALARQIPSADRSTRAGKWEKSRFMGVELAGKTLGLIGCGNIGSIVAERAQ
                     GLKMRVIVYDPYLSPERAGDLNVEKVELDDLLARAHVISLHTPLNDATRGMINAAALA
                     KTRPGVRIVNCARGGLVVETDLKAALLSGHVAGAALDVFESEPARANPLFGLEQVIAT
                     PHLGASTSEAQEKVAVQVAEQMADYLSSGAVTNAVNMPSLTAEEAPRLMPYMKLADQL
                     GSFAGQVIETGLRAVSVQFEGHAATLNCRPLTAVVLRGLLKSQLDSVNMVNAPLVAKE
                     RDIEVRETLYDGPCDYHTLIRLNLVTEQHTRTLAGTLLGAHPRITEIEGIAVDATLGP
                     SMLYINNRDTPGMIGAFAKMLGDAGINIATFHLGRRAPGGDAIALVEVDQPVPEALVG
                     AVRALPGVVRVKPLRF"
     gene            complement(2111..3280)
                     /locus_tag="VGB88_01285"
     CDS             complement(2111..3280)
                     /locus_tag="VGB88_01285"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX9646099.1"
                     /translation="MAAMKPSRRPANPNFSSGPCAKRPGWSAAVLEAALVGRSHRSGA
                     GKARLGEVIRSSRDLLGLPGDWRLAIMAGSDTGAFEAAMWSLLGARGVDVLAWESFGK
                     GWLTDVVEQLKLADVRSFEADYGSLPDLGAIDCDRDVVFTWNGTTSGVRVPDGDWIAA
                     DRSGLTLCDATSAAFAMALPWDKLDVATWSWQKVLGGEAQHGMLALSPRAAARLEGTT
                     PARPLPKIFRLTKGGKLIEGTFEGETINTPSMLCVEDALDGLRWAASIGGVPALIARS
                     QANLAAIAAWVRATPWVAFLAEDAAIRSSTSVCLKIVDPWFGALPEPAQRATAKRVAK
                     ALEDEGVAFDIAAYRDAPPGFRLWAGATVEAGDIEAVLPWLDWAYGGVRAEARAA"
     gene            3683..4663
                     /locus_tag="VGB88_01290"
     CDS             3683..4663
                     /locus_tag="VGB88_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9646100.1"
                     /translation="MTATEPPMLVNFVLWLMRRSPAARRLAWRGFHELLGHRYGGLDW
                     WTFMNYGFAELGEDPRPIPLAPEDEAERYCAQLYAHVTGAVDLEGRDVLEVGCGRGGG
                     VSYVARYLNPRRATGLDAARTQVDFCRRVHGGPKVEFVHGEAERLPFDDDSFDAVVNV
                     ESSFCYRDMDAFLAEVRRVLRPGGHFLFADVRLAFEADALVDQLARAGFTVIGARDIS
                     GNVCRALELDAERRRKVAATLVPAYLRHVLYAFVGVEGTRIPTLLSSGLLRYLSVTMQ
                     KDGAQLAAPIRGTVAARTSEPRELVGAEQAARQGARVAGFELTQRDSPAA"
     gene            complement(4665..5489)
                     /gene="thiD"
                     /locus_tag="VGB88_01295"
     CDS             complement(4665..5489)
                     /gene="thiD"
                     /locus_tag="VGB88_01295"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX9646101.1"
                     /translation="MTGRVLIVAGSDSGGGAGIQADIKTVTALGGYAACAITALTAQN
                     TLDVYGIHEVPADFVAEQMVVVLDDIGADALKTGMLHRRDVIEAVARVIAERASDVPL
                     VVDPVMVAKSGTRLITEDAQGALADRLLPLARLVTPNLPEAARLAGMDIEAEADMAEA
                     ARRILALGPKAVLVKGGHLEGETLVDLLVDGEGARRFTGPRIASRNTHGTGCTLASGI
                     ATGLAQGMALADAVARARAYVAAAIRTAPGLGAGHGPLDHAHPLAGCTGARKGDAA"
BASE COUNT          786 a         2019 c         1994 g          850 t
ORIGIN      
        1 gcgtgcggat cgtccttgat accaacgtcc tgatcggcgc cctgatcacc aaaggcacgc
       61 cgccggaccg gttgtaccag gcatggctgc gcggcgagat cgaccttgtc acgtgcacgg
      121 ctcaaatcgt cgaggtcgcc gatgttctca gccggccgcg cctgctgaag tatgtcgacg
      181 ccgacgaggc ggcggcgatc gttgagaaca tcgacacacg cgccgttatc cttgacgacc
      241 cgccggacgt ggacctctcg ccggatccca aggacaaccc gatcttggcc gcggcgatcg
      301 ccggcaaggt tgacttgatc gtctcgggcg acaagaaaca catgctcgcg ttcggcgagg
      361 tcgaaggcat ccccatcgtg acggcccgtg acgccttgga ccgcctgagc aaggaataat
      421 tggcctgcgc tgcggcttgg tcgcccgtcg tccaacgact ccttcttgct aatccgcgac
      481 gtgcatggcg ctccgccaga aggctcgatt taggttccgg gctcagaacc tgagcggctt
      541 gacgcgcacc acgccgggca gcgcgcgcac cgcgccgacc agcgcctcgg gcaccggctg
      601 gtcgacctcg accagggcga tggcgtcgcc gcccggcgcc ctgcggccga gatggaaggt
      661 cgcgatgttg atgccggcgt cccccagcat cttggcgaac gcgccgatca tgcccggggt
      721 gtcccggttg ttgatgtaga gcatgctcgg cccgagcgtc gcgtcgaccg cgatgccctc
      781 gatctcggtg atgcgcgggt gcgcgccgag cagcgtgccg gcgagcgtgc gcgtgtgctg
      841 ctcggtgacc aggttgagcc ggatcagcgt gtggtagtcg catggcccgt catagagggt
      901 ctcgcgcacc tcgatgtcgc gctccttggc gaccagcggc gcattgacca tgttgaccga
      961 gtcgagctgg gacttgagca ggccccgcag gaccaccgcg gtcagcggcc ggcagttgag
     1021 ggtggccgcg tggccctcga actggaccga caccgcgcgc aggccggtct cgatcacctg
     1081 gccggcgaag ctgccgagct ggtcggcgag cttcatgtac ggcatcagcc gcggcgcctc
     1141 ctcggcggtg agcgagggca tgttgaccgc attggtcacc gcgccgctcg aaagatagtc
     1201 ggccatctgt tcggccacct gcaccgccac cttctcctgc gcctcgctgg tcgaggcgcc
     1261 gagatgcggg gtcgcgatca cctgctcgag gccgaacagc ggattggcgc gcgccggctc
     1321 ggactcgaac acgtcgagcg cggcgccggc gacatgcccg gacagcagcg ccgccttgag
     1381 gtcggtctcg accaccagcc cgccgcgggc gcagttgacg atgcgcaccc cgggccgggt
     1441 cttggccagc gcggcggcat tgatcatgcc gcgggtggcg tcgttgagcg gcgtgtgcag
     1501 gctgatgacg tgggcgcgcg caagcagatc gtcgagctcg accttctcaa cgttgaggtc
     1561 gcccgcgcgc tcgggcgaca gatacgggtc gtagacgatc acccgcatct tcaggccctg
     1621 cgcgcgctcg gcgacgatcg agccgatgtt gccgcagccg atcaggccga gcgtcttgcc
     1681 ggcgagctcg acgcccatga agcgcgactt ctcccacttg ccggcccggg tcgagcggtc
     1741 ggcgctcggg atctggcgcg ccagcgcaaa gatcatggcg agcgtgtgct cggcggtggt
     1801 caccgagttg ccgaacgggg tgttcatcac caccaccccg cgcgcggtcg ccgcggcgac
     1861 gtcgatgttg tcgacgccga tgccggcgcg cccgaccacc ttgagccggc ggccggcgtc
     1921 gagcaccgcg gcggtgacct tggtcgccga acgcaccgcg agcccgtcat agccggcgat
     1981 gcgcgcgatc agctcatcgg ccgcgagccc gggcgccacg tcgacctcga tgccgcgctc
     2041 gcggaacaca tcggccgcgc tcgggctcaa cgaatccgcg atcaggacct tggccatggc
     2101 ggcggcggcg tcaggcggcc cgggcctcgg cgcgcacgcc gccataggcc cagtcgagcc
     2161 acggcagcac tgcctcgatg tcgccggcct cgacggtcgc gccggcccac agcctgaagc
     2221 cgggcggggc gtcgcgatag gcggcgatgt cgaaggcgac gccttcgtcc tcgagcgcct
     2281 tggcgacccg cttcgcggtc gcgcgctgcg ccggctcggg aagcgcgccg aaccacggat
     2341 cgacgatctt gaggcacacc gaggtcgacg agcggatcgc cgcgtcctcg gccaggaagg
     2401 cgacccacgg ggtcgcccgc acccaggccg cgatcgcggc gaggttggcc tggctgcgcg
     2461 cgatcagcgc cggcacgccg ccgatcgagg cggcccagcg caggccgtcg agcgcgtcct
     2521 cgacgcacag catcgacggc gtgttgatgg tctcgccctc gaaggtgccc tcgatcagct
     2581 tgccgccctt ggtgagtcgg aagatcttgg gcagcggccg ggccggggtg gtgccctcga
     2641 ggcgggcggc ggcgcgcggg ctcaaggcca gcatgccgtg ctgggcctcg ccgccgagca
     2701 ccttctgcca cgaccaggtg gcgacgtcga gcttgtccca gggcagcgcc atggcgaagg
     2761 cggccgaggt cgcgtcgcac aacgtgagcc ccgagcggtc ggcggcgatc cagtcgccgt
     2821 cgggcacgcg cacgcccgag gtggtgccgt tccaggtgaa caccacgtcg cggtcgcagt
     2881 cgatggcgcc gaggtcgggc agcgacccgt agtcggcctc gaagctgcgc acgtcggcga
     2941 gcttgagctg ctcgaccacg tcggtgagcc agcccttgcc gaagctctcc caggcgagca
     3001 cgtcgacgcc gcgcgcgccc aggagcgacc acatcgccgc ctcgaaggcg ccggtatcgg
     3061 agcccgccat gatcgcgagc cgccagtcgc cgggcaggcc gagcaggtcg cggctcgagc
     3121 gaatcacctc acccagccgc gccttgccgg cgcccgagcg gtgcgagcgc ccgaccagcg
     3181 ccgcctccag cacagccgcc gaccaccccg gccgcttggc gcacgggccg gatgagaagt
     3241 tgggattggc cgggcggcgg ctcggcttca tggcggccat cgttttgctt atggtgcagt
     3301 gcaaaaagcg gcgcggagaa taggcgcgca ccccggccgc gtcaacatgc cggcgccgcg
     3361 cccgccgacc gccgccggcg agagttcgac cctgaacaaa tcttccgctc gcgggccgcg
     3421 ccggcttgac cgcgcgcctg ttcgacgcta tgcggtcttc gctgtcgact cgcggggtag
     3481 taaagccgag cctggatcgc gaccgaaata tcccgcggca cctcgtgttc ccagatcctt
     3541 gtgacttttg acggggtttg acgtgttgtt gacgcggtcg cggcgcgcga cgaacggcca
     3601 tctgacgccc gcgccgtaga ctgcgacctg ttgcaatcgc cgcggtcggc cgtcacggcg
     3661 ccgccgcact cggacgcttg ttgtgaccgc gacggagccg cccatgctgg tcaatttcgt
     3721 tctttggctg atgcgccgtt cgccggccgc gcgccggctc gcctggcgcg gattccacga
     3781 gctgctgggg caccgctatg gcgggctcga ttggtggacg ttcatgaact acggctttgc
     3841 cgagctcggc gaggacccgc ggccgatccc gctcgcgccg gaggacgaag ccgagcgcta
     3901 ttgcgcgcag ctctacgccc acgtgaccgg ggccgtcgac ctcgaaggtc gcgacgtgct
     3961 cgaggtcggc tgcgggcgcg gcggcggcgt ctcctacgtc gcccgctatc tcaacccgcg
     4021 ccgcgccacc gggctcgatg cggcgcgcac gcaggtcgac ttctgccgcc gcgtccatgg
     4081 cgggccgaag gtcgagttcg tgcacggcga ggccgagcgc ctgccgttcg acgacgacag
     4141 cttcgatgcc gtggtcaacg tcgagtcgtc gttctgctac cgcgacatgg acgccttcct
     4201 ggccgaggtc cggcgcgtgc tcaggcccgg cggccatttc ctgtttgccg acgttcgcct
     4261 cgccttcgag gccgacgcgc tggtcgatca gctcgcgcgc gcggggttca ccgtgatcgg
     4321 ggcgcgcgac atctcgggca atgtctgccg ggcgctcgag ctcgacgcgg agcgccggcg
     4381 caaggtggcg gcgacgctgg tgccggccta cttaaggcac gtcctttatg ccttcgtggg
     4441 cgtcgagggc acgcgcatcc cgaccctgct gtcgtccggc ctgctgcgct atctgagcgt
     4501 gaccatgcag aaggacggcg cgcagctggc ggcgccgatc cgcggcacgg tggcggcgcg
     4561 tacgagcgag ccgcgcgagc tggtcggcgc cgagcaggcc gcccgccagg gcgcgcgcgt
     4621 ggccggattc gagctaaccc agcgcgactc gcccgccgcc tgactcacgc ggcatcgccc
     4681 ttgcgtgcgc cggtgcagcc ggcgagcgga tgcgcgtggt cgagcggccc gtggccggcg
     4741 ccgaggccgg gggcggtgcg gatcgccgcg gcgacgtagg cgcgcgcgcg cgcgaccgcg
     4801 tcggcgagcg ccatcccttg ggcgaggccg gtggcgattc ccgaggcgag cgtgcagccg
     4861 gtgccgtggg tgttgcggct ggcgatccgc ggcccggtga accggcgcgc gccctcgccg
     4921 tcgaccagca ggtcgaccaa ggtctcgcct tccagatgcc cgcccttgac caggaccgcc
     4981 ttcggcccga gcgcaaggat gcgccgcgcc gcctccgcca tgtcggcctc ggcctcgatg
     5041 tccatgccag caaggcgcgc cgcctcgggg aggttcgggg tgaccaggcg ggcgagcggc
     5101 agcagccggt cggcgagggc gccttgcgcg tcctcggtga tcaggcgcgt gccgcttttc
     5161 gccaccatca ccgggtcgac caccagcggc acgtcactcg cgcgttcggc aatcacgcgc
     5221 gccaccgcct cgatcacgtc gcggcggtgg agcatgccgg tcttgagcgc atccgcgccg
     5281 atgtcgtcga gcaccacaac catttgctcg gcaacgaaat cggccggcac ctcgtggatg
     5341 ccgtagacgt ccagggtgtt ctgggcggtg agcgcggtga tcgcgcacgc ggcatagccg
     5401 ccgagcgcgg tgacggtctt gatgtcggcc tggataccgg cgccgccgcc cgagtcggac
     5461 ccggcgacga tgaggacccg gcccgtcacg atgcgtcgcc ggtcatgccg cgccccccca
     5521 tcgcccgccc acccggtctc agtgcgcgac gctctcgatc gcgtcgacgc ccaggacaat
     5581 cccctcacca atatctacaa cttcggcgtc cacctccacc accgctacat cacgctctcc
     5641 gggcatttc
//
LOCUS       DASWHU010000038         7021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_12318, whole genome shotgun sequence.
ACCESSION   DASWHU010000038 DASWHU010000000
VERSION     DASWHU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12318"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGB88_01300"
     CDS             complement(<1..343)
                     /locus_tag="VGB88_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9646102.1"
                     /translation="MEARVEWVGERTFVGISGSGHGLVMGARYQGRPAPGPSPMEMLL
                     LGLAACTAFDVVHILERGREPVTGCTVAVEAERAETDPQVFTRIHLSYVVTGKGLSRA
                     KVERAVQLSEEK"
     gene            complement(364..1341)
                     /locus_tag="VGB88_01305"
     CDS             complement(364..1341)
                     /locus_tag="VGB88_01305"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9646103.1"
                     /translation="MAIATRLTARLGLEHPIILAPMAFAGGGRLAAAVSRAGGLGLIG
                     GGYGDGDWLEAEFAAAGNTPVGCGFITWSLARAPHLLDAVLARAPRAVMLSFGDPAQF
                     APAIRAAGATLICQVQSLAHARAALDAGAEVIVAQGAAAGGHSAVRATVTLVPEVADL
                     CAAVAPETLVVAAGGIADGRGLAAALVLGADGVLMGSRFVAAEEALVTRAFQDAAVEA
                     CGDDTLRTRTVDIVRRRDWPAEFDIRVLRNAFVDRWHGNETALAAAVEVEAPRYQEAL
                     ARGDTERAAVVVGEAVGLIREVMPAAEIIRRVVVAAEARLEAGAKLARR"
     gene            complement(1507..2313)
                     /locus_tag="VGB88_01310"
     CDS             complement(1507..2313)
                     /locus_tag="VGB88_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX9646104.1"
                     /translation="MSDSGGYGDKRFSLIAALGLLVALASAVALAVGAYGYREQMFDV
                     RFALLTVNQYASYAGAVAAALGLIAIGATLPGGRRRGFVIALLALVIGGGAAGYIGYL
                     YWQVETLPYIHDITTDLENPPQFVAATAVRVREGAPNPPEYDGPDAALQQRYGTLRGR
                     EGYTGLEPLVVAVPAAEVFDKALALIEQRGWEVIESDGTAGRIEATDTSRFYLFKDDV
                     VIRLTPSGEGTAIGTRIDMRSKSRIGRSDLGVNAQRIAEFLGALRNAVGG"
     gene            2590..2766
                     /locus_tag="VGB88_01315"
     CDS             2590..2766
                     /locus_tag="VGB88_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646105.1"
                     /translation="MTATEIKDSSRRTEALQSQLASVLFQFYNGELADDDEYRRVVNA
                     LCKALLDLDTARKN"
     gene            complement(2773..4974)
                     /gene="ligA"
                     /locus_tag="VGB88_01320"
     CDS             complement(2773..4974)
                     /gene="ligA"
                     /locus_tag="VGB88_01320"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX9646106.1"
                     /translation="MSRPRPADKPAEALTEAEAAAELARLAKEIAHHDRLYYQQDRPE
                     VSDAAYDALRLRNQAVEARFPALIRADSPSRRIGAAPAAGFAKVRHRVPMLSLDNAFD
                     ADDVARFFARVRRFLKLGPDDPVEVVGEPKIDGLSIAVRYEHGRFVQGATRGDGREGE
                     DVTRNLATVGDLPKRLKGAAPAVLEVRGEVYMRKPEFAALNERRRGAGEPEFANPRNA
                     AAGSLRQLDPTVTAGRPLHLFAYSYGEVEGAALGETHWQFLARLRDWGLPTNPLARLC
                     RAPEDALALHDEIEGERANLPYDIDGVVYKINRFDYQERLGFVSRAPRWAIAHKFPAE
                     QAETVLDDIEINVGRTGTLTPVAILRPVTVGGVVVSRATLHNEDEIARKDIRIGDHVV
                     VQRAGDVIPQVVRVIASKRPKGARPYIFPTICPCPLKTPAVRKEGEAARRCTGELACP
                     FQQVERLIHFASRDAFDIEGLGEAYIQLFFDRGLVTDAADIFTLEARSGEVNRVVAEW
                     HERQSAARQAETGRAKAAKRKKQDDYKSVRNLFAAIEARRSIDLHRFIYALGIRQVGE
                     ATARLLAGTYLTLKDWQTGMIDAAKERAARADAGKPDQVGPAYARLCETEGVAMSVAD
                     DICEFFSEPHNLKVIKALESEITVTPAEAPAAGGSPVAGKTVVFTGSLATMTRDEAKA
                     QARALGAKVAGSVSAKTDYVVAGEEAGSKLAKARELGVAVLSEDEWRRLIG"
     gene            complement(4971..6659)
                     /gene="recN"
                     /locus_tag="VGB88_01325"
     CDS             complement(4971..6659)
                     /gene="recN"
                     /locus_tag="VGB88_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9646107.1"
                     /translation="MLVRLSIRNVVLIERLDIDFGPGLCVLTGETGAGKSILLDALGL
                     ALGRRAEAGLVGAAPERASAAASFEIDAGHPARRWLAEHGLDDEGALVLRRALGADGR
                     SRAFINDQPVTVAALAELGALLVEVYGQHDRLGLMDPATHRRALDSFGGLEGARVHTA
                     AAHRARVAAEQALAVAEADAAAARARREEIEAVVADLRALAPVAGEEQRLAEERAALM
                     ARGRIGELLGQAAELVAGSGRAADEALRGAQRLVAQAAAQAAGRLEALAAAFERAVVE
                     TAEALALLDAAGAGLDAEPGRLDAVEERLFALRAAARRHGVAVDALPELARTLADRLE
                     ALAEGDAARRDLAEAAVAARAVYDEAAAALSRGRAKAAKRLDRAMGAELAGLKLAAAG
                     FATRLEPLAEARRGATGAERISFEVATNPGLAPGALSDIASGGELSRFMLALCVVLAD
                     SRSGGALIFDEIDAGIGGAVADAVGRRLGRLAGSRQVLVVTHQPQVAALADRHLRVVK
                     SVEAGRTLTRIEALDSEARREEIARMLAGAEITDQARAAADSLLDGRAERAAAT"
     gene            complement(6882..>7021)
                     /locus_tag="VGB88_01330"
     CDS             complement(6882..>7021)
                     /locus_tag="VGB88_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9646108.1"
                     /translation="EAQAAAAVLGHNFPGSAWYADAYELLQERELEPRLSESSWLARL
                     F"
BASE COUNT          954 a         2675 c         2468 g          924 t
ORIGIN      
        1 acttctcctc ggacagctgg accgcgcgct cgaccttggc gcgcgacagg cccttgccgg
       61 tgaccacgta gctcaagtgg atgcgggtga acacctgcgg gtcggtctcg gcgcgctcgg
      121 cctcgaccgc gaccgtgcac cctgtcaccg gctcgcggcc gcgctcgagg atgtgcacca
      181 cgtcgaaagc ggtgcacgcg gcgagcccga gcagcagcat ctccatcggg ctcggcccgg
      241 gggcggggcg gccctggtag cgcgcgccca tgaccagccc gtggccgctg cccgagatgc
      301 cgacgaaggt ccgttcgccc acccattcga cccgcgcttc catcgtccag cctccccggg
      361 aacctagcgg cgcgcgagct tcgcgcccgc ctcgagccgc gcctcggccg cgacgaccac
      421 gcggcggatg atctcggccg ccggcatcac ctcgcggatc aggccgaccg cctcgccgac
      481 caccaccgcc gcccgctcgg tgtcgcctcg cgccagcgcc tcctgatagc gcggcgcctc
      541 gacctcgacc gccgcggcca gcgccgtttc attgccgtgc caacgatcga cgaaagcgtt
      601 gcgcaggaca cggatatcga actccgccgg ccagtcgcgg cggcgcacga tgtcgaccgt
      661 gcgggtgcgc agcgtgtcgt cgccgcaggc ctcgacggcg gcgtcctgga acgcgcgcgt
      721 caccagcgcc tcctcggcgg cgacgaagcg cgagcccatc agcacgccgt cggcgccgag
      781 caccaaggcg gcggcgaggc cgcggccgtc ggcgatgccg ccggcagcga ccacgagcgt
      841 ctcgggcgcg accgcggcgc acaggtcggc gacctcgggc accagggtca cggtagcgcg
      901 cacggcactg tggccgccgg ccgccgctcc ttgcgcgacg atgacctcgg cgccggcgtc
      961 gagcgccgcg cgcgcatgcg ccaggctctg cacctggcag atcagcgtcg cgccggcggc
     1021 gcggatcgcg ggcgcaaatt gcgccgggtc gccgaacgac agcatcaccg cccgcggcgc
     1081 gcgggcgagc acggcatcga gcaggtgcgg cgcgcgggcg agcgaccagg tgatgaagcc
     1141 gcagccgacc ggcgtgttgc cggcggcggc gaactcggcc tccagccagt cgccgtcacc
     1201 gtagccgccg ccgatcagcc cgagcccgcc ggcgcgcgag accgccgccg ccagccggcc
     1261 gccgccggcg aacgccatcg gcgccaggat gatcggatgc tcgagcccga gccgggcggt
     1321 cagccgggtc gcgatcgcca cgtccgcgcc gctcagagcc ggaaaatctc tcgcctcacc
     1381 cttcgagacg cgcccagtgg gcgctcctca gggtgaggta cctttttgat tttcaacatg
     1441 tcctcgtcct gaggagcgag cgcagcgagc gtctcgaagg accaaacccg gttcattcac
     1501 gcgctctcag cccccgaccg cgttcctcag cgcgcccagg aactcggcga tgcgctgggc
     1561 gttgaccccg aggtcggagc ggccgatgcg gcttttcgag cgcatgtcga tgcgggtgcc
     1621 gatcgcggtg ccctcgccgc tcggcgtcag ccggatcacc acgtcgtcct tgaacaggta
     1681 gaaccggctg gtgtcggtgg cctcgatccg accggccgtg ccgtccgatt cgatgacctc
     1741 ccagccgcgc tgctcgatca gcgccagcgc cttgtcgaac acctcggccg ccggcaccgc
     1801 gaccaccagc ggctcgaggc cggtatagcc ctcgcgcccg cgcagcgtgc cgtagcgctg
     1861 ctgcagcgcc gcatccgggc cgtcatattc gggcgggttc ggcgcgccct cgcgcacacg
     1921 caccgcggtc gcggcgacga actgcggcgg gttttcgaga tcggtggtga tgtcgtggat
     1981 gtagggcagg gtctccacct gccagtagag atagccgata tagcccgccg cgccgccgcc
     2041 gatgaccagc gccaagagcg cgatgacgaa gccgcgccgc ctgccccccg gcagggtggc
     2101 gccgatcgcg atcagcccga gcgcggccgc caccgcgccg gcataggacg cgtactggtt
     2161 gacggtgagc agcgcgaacc ggacatcgaa catctgctcg cggtagccgt aggcgccgac
     2221 ggcgagcgcg accgccgacg ccaatgccac cagaaggccg agcgcggcga tgagcgagaa
     2281 ccgcttgtcg ccataaccac cggagtcgga catgcctgct tctccccttc ccccgccggc
     2341 gccgaagagc gccgggcccc ggggccatgt ttccaccgcc tcgacgacaa atcaaccgag
     2401 actggaatcc cgccgcccgc cacccgcgcc atggctgcaa cggcggcgcc cggcagcggc
     2461 cggcgagcgg gcgccggggc cattttgggc cgcgggtgtg accggcatca ctgcgacttg
     2521 cgcgcccccg gaccatcctc actgggacgg atcgggagaa cccgatcgag gttcgccgga
     2581 gaccgaagca tgaccgcaac cgagatcaag gacagctcgc gtcgaaccga ggcgctgcag
     2641 agccagctcg ccagcgtgtt gtttcagttc tacaacggcg agctcgcgga cgacgacgag
     2701 taccggcgcg tggtcaacgc cttgtgcaag gcgctgctcg acctcgacac cgcgcgtaag
     2761 aactgagcgg cctcagccga tcagcctgcg ccactcgtcc tcgctgagca ccgccacccc
     2821 cagctcgcgc gccttggcga gcttggagcc cgcctcctcc cccgccacca cgtaatcggt
     2881 cttggccgac accgagcccg cgaccttggc gccgagcgcg cgcgcctgcg ccttggcctc
     2941 gtcgcgggtc atggtcgcca ggctgccggt gaacaccacg gtcttgcccg cgaccggcga
     3001 gcccccggcg gcgggcgcct cggccggcgt gacggtgatc tcggactcga gggccttgat
     3061 caccttcagg ttgtggggct ccgagaagaa ctcgcagatg tcgtcggcga ccgacatggc
     3121 cactccttcg gtctcgcaca ggcgcgcata ggcgggcccg acctggtccg gcttgcccgc
     3181 gtccgcccgc gccgcgcgtt ccttggccgc atcgatcatg ccggtctgcc agtccttgag
     3241 cgtcaggtac gtgccggcca ggagccgcgc cgtcgcctcg ccgacctggc ggatgccgag
     3301 cgcgtagatg aaccggtgga gatcgatcga gcgccgcgcc tcgatggcgg cgaacaggtt
     3361 gcgcacgctc ttgtagtcgt cttgcttttt ccgcttggcc gccttggccc ggcccgtctc
     3421 ggcctggcgc gccgcgctct ggcgctcgtg ccactccgcg accacccggt tgacctcgcc
     3481 ggagcgcgcc tcgagggtga agatgtcggc agcgtccgtg accagccccc ggtcgaagaa
     3541 cagctggata tacgcctcgc cgagaccttc gatgtcgaag gcgtcgcggg aggcgaagtg
     3601 aatcagccgc tcgacctgct gaaaggggca ggcgagctcg cccgtgcagc gccgcgcggc
     3661 ctcgccctcc ttgcgcaccg cgggcgtctt gagcgggcac gggcaaatgg tcgggaaaat
     3721 atagggtctt gcgcccttcg gccgcttgct cgcgatcacg cgcaccacct gcgggatcac
     3781 gtcgccggcg cgctggacga cgacgtggtc gccgattcgg atgtccttgc gcgcgatctc
     3841 gtcctcgttg tgcaaggtcg cgcggctgac cacgacgccg ccgaccgtga ccgggcgcag
     3901 gatggcgacc ggggtcagcg tgccggtgcg cccgacgttg atctcgatgt cgtcgagcac
     3961 cgtctccgcc tgctcggcgg ggaacttgtg ggcgatcgcc cagcgcggcg cgcggctgac
     4021 gaagccgagc cgctcctgat aatcgaagcg gttgatcttg tagaccacgc cgtcaatgtc
     4081 gtagggcagg ttggcgcgct cgccctcgat ctcgtcgtgg agcgcgagcg cgtcctcggg
     4141 cgcgcggcac aggcgcgcca atgggttggt cggcaggccc cagtcgcgca ggcgggcgag
     4201 gaactgccaa tgggtctcgc ccagcgccgc gccctcgacc tcgccgtaag aataggcgaa
     4261 caggtgcagc gggcgcccgg cggtgaccgt cgggtcgagc tggcgcaggc tgcccgcggc
     4321 ggcgttgcgc gggttggcga actcgggctc gcccgcgccc ctgcgccgct cgttgagcgc
     4381 ggcgaactcg ggcttgcgca tatagacctc gccgcgcacc tcgagcaccg ccggcgccgc
     4441 gcccttgagg cgcttgggca ggtcgccgac ggtggcaagg ttgcgggtca cgtcctcgcc
     4501 ctcgcggccg tcgccgcggg tcgcgccctg gacgaagcgg ccatgctcgt agcgcaccgc
     4561 gatcgacagc ccgtcgatct tgggctcgcc caccacctcc accggatcgt ccgggccgag
     4621 cttgagaaag cggcggacgc gggcgaagaa gcgcgccacg tcgtccgcgt cgaaggcgtt
     4681 gtcgagcgac agcatcggca cgcgatggcg aaccttggcg aacccggccg ccggcgccgc
     4741 gccgatccgc cgcgacgggc tgtcggcgcg gatgagcgcg gggaagcgcg cctcgaccgc
     4801 ctggttgcgc aggcgcagcg cgtcgtacgc ggcgtccgag acctcgggcc ggtcctgctg
     4861 gtagtacagg cggtcgtgat gcgcgatctc cttggccagc cgggcaagct cggcggccgc
     4921 ctcggcctcg gtcagcgcct ccgccggctt gtcggcgggc cgcggccggc tcatgtcgcc
     4981 gcggcgcgct cggcgcgtcc gtcgagcagg ctgtcggcgg cggcgcgcgc ctggtcggtg
     5041 atctcggcgc cggccagcat gcgcgcgatc tcctcgcgcc gggcttccga atcgagcgcc
     5101 tcgatgcggg tcagggtccg tcccgcctcg acgctcttga ccacgcgcaa atgccggtcg
     5161 gcgagcgccg ccacctgcgg ctggtgggtg accaccagca cctggcgcga gccggccagg
     5221 cggcccagcc ggcggccgac cgcgtcggcg accgcgccgc cgatgccggc gtcgatctcg
     5281 tcgaagatca gcgcgccgcc gctgcggctg tcggcgagca cgacgcacag cgcgagcatg
     5341 aaccgcgaca gctcgccgcc cgaggcgatg tcgctcagcg cccccggcgc aagccccgga
     5401 ttggtcgcca cctcgaagct gatgcgctcg gcgccggtgg cgccccggcg cgcctcggcc
     5461 aacggctcga gccgggtggc gaagccggcg gcggcgagct tcagcccggc aagctcggcg
     5521 cccatagccc ggtcgagccg cttcgccgcc ttggcgcggc cacgcgacag cgccgcggcc
     5581 gcctcgtcat agacggcgcg cgccgccacc gcagcctcgg ccaggtcccg gcgcgccgcg
     5641 tcgccctcgg cgagcgcctc gagcctgtcg gcgagggtgc gggcaagctc gggcaaggca
     5701 tcgaccgcga cgccgtgccg gcgtgccgcg gcgcgcagcg cgaacagccg ttcctcgacc
     5761 gcgtccagcc gcccgggctc ggcgtcgagc ccggcgcccg cggcatcgag cagggcgagc
     5821 gcctcggccg tctccaccac cgcccgctcg aacgccgcgg ccagcgcctc gagccgcccc
     5881 gccgcctggg cggccgcctg ggcgaccagc cgctgggcgc cgcgcagcgc ctcgtcggcg
     5941 gcgcgccccg agcccgcgac cagctctgcc gcctggccga gcagctcgcc gatccgcccg
     6001 cgcgccatca aggccgcgcg ctcctctgcc aggcgttgct cctcgccggc gaccggcgcg
     6061 agcgcacgca ggtccgcgac cacggcctcg atctcctcgc gccgcgcgcg cgcggcagcg
     6121 gcgtccgcct cggcaacggc caacgcctgc tcggcggcga cgcgggcgcg gtgcgcggcc
     6181 gcggtgtgta cgcgcgcgcc ctccaggccg ccgaacgaat cgagcgcgcg gcgatgggtc
     6241 gccgggtcca tcaggccgag ccggtcgtgc tggccgtaga cctcgaccag cagggcgccg
     6301 agctcggcga gcgccgccac cgtcaccggc tggtcgttga tgaaggcgcg gctgcggccg
     6361 tccgcgccca aggcgcggcg cagcaccagc gcgccctcgt cgtcaaggcc gtgctcggcg
     6421 agccagcggc gggcgggatg gccggcgtcg atctcgaacg acgccgccgc gctcgcccgc
     6481 tcgggcgccg cccccaccag ccccgcctcg gcgcgccggc cgagcgccag gccgagcgcg
     6541 tcgagcagga tcgacttgcc ggcaccggtc tcgccggtca gcacgcacag cccgggcccg
     6601 aaatcgatat cgagccgctc gatcaagacc acgttgcgga tcgacaggcg taccagcatg
     6661 gctgacgtcc gcgccgcgtg cgccgtcacc ggcgcgggcg cgctcgtatc cgggaaccca
     6721 aattgcggct gcatgcccca tccttcgaga cgggccttcg gccctcctca ggatgaggct
     6781 ccgagcatgc gtatccgatg acaaacctca tgctaaggag cccgcagagc gggcgtctcg
     6841 aagcatgcgg cctgcgactc atgcgccaac gatagtcggc gctagaacag tcgcgccagc
     6901 cacgagcttt cgctcagccg cggctccagc tcgcgctcct gcagcagctc gtaggcgtcg
     6961 gcgtaccacg cgctgccggg aaaattgtgg ccgagcacgg ccgcggccgc ctgcgcctcg
     7021 c
//
LOCUS       DASWHU010000039         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1489363, whole genome shotgun sequence.
ACCESSION   DASWHU010000039 DASWHU010000000
VERSION     DASWHU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489363"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VGB88_01335"
     CDS             complement(<1..1073)
                     /locus_tag="VGB88_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9646109.1"
                     /translation="MDFRLSDEQRALQEAARKFARGELEGVARELERDNRPLPDGVRR
                     RYAELGFLGINVAHVYGGLGLGNLEALLVVEEFAKVSSAVAFPVFESCVGPVKAIEAF
                     GSDSLRRRVLPAVCRGEVVVAVSMSEPDAGSALTDLTTRARREGDEIVVSGHKRWTSG
                     GGHAHGYLVYCRLSNQPGAKGIGAVYVEKDRAGVSFGPNETLMGFRGVPSADIHLDEV
                     RVPADNLVVDAGGFARLMQAFDLERCGNATMALGQAQAALDDALAYVQERRQFGKPLV
                     EFQAVQLKLAEMAMRVDAARLLIHRAAVNSGQGLPSLKESSLAKCYANEIAREVCGAA
                     LQLMGGYGYSTEFAIERRFRDSWG"
     gene            1204..1689
                     /locus_tag="VGB88_01340"
     CDS             1204..1689
                     /locus_tag="VGB88_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9646110.1"
                     /translation="MHAFARIAVVVLCALSFAAVTGAASFAADEPANIIKYRRNVMKA
                     IGANIANIAMVVKGEVTYSANVPFNARSIRDALNLVDPLFPPGTDTGDTNALPKIWED
                     AAGFNDVHDKAQAAADAMVQAAESGDAANIAAALGDLGKACGNCHKPYRVDSSSAAWR
                     P"
     gene            1677..2606
                     /locus_tag="VGB88_01345"
     CDS             1677..2606
                     /locus_tag="VGB88_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9646111.1"
                     /translation="MAAIGAAAGIGVGLAAAGAFALAAAAADEAVARGAYLARAGGCF
                     ACHTDVKGGGAPLAGGGRIETPFGTFFGPNLTPHPDHGLGRWAEADFVAAMTEGRAPD
                     GARYYPAFPYTSFSATARADLADLWSYLRSLAPVARANTPHELKWPFGWRFLNRIWQW
                     LFFRPQTFAPDPAHDAHWNRGAYLSEALLHCKECHSPRSRLGALKISLAYAGNRDGPG
                     DTTVPNITADAETGIGRWSEIDVVWLLKTGFKPDGNDVQGIMAELIEHGSSHLSDDDL
                     TAVAAYIRSLEPIRYQVRKPRAESGTPGGEFNY"
     gene            2850..>3859
                     /locus_tag="VGB88_01350"
     CDS             2850..>3859
                     /locus_tag="VGB88_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9646112.1"
                     /translation="MTELALCRRPAALLLALAVVAAPTLADARPVPDSFADLVERLSP
                     AVVAVSTEEVVATGGQLPFQFPPGSPFEEFFRRYFGAPPEDQGGAPEQRRMSAGSGFI
                     VDSAGYIVTNNHVIADADTITVTLADESEFEATLIGSDPSTDVALIKIDAGHPLATVV
                     WGDSDQVRVGDWTLAIGNPFGLYGSVTAGIISARARTLNAGPFDDFLQTDASINPGNS
                     GGPLFNLAGEVIGINTAIFSPSGGNIGIGFAIPSALAKPVVDQIRDHGRAIRGWLGVQ
                     IQGVTKELAAGLGVKEAYGALVVKVSDDSPAARAGLKTGDLIVGYRGQRVDDPRALAR
                     MVA"
BASE COUNT          568 a         1454 c         1286 g          551 t
ORIGIN      
        1 ccccagctgt cgcggaaccg ccgctcgatc gcgaactcgg tcgagtagcc gtagccgccc
       61 atcagctgca gcgccgcccc gcacacctcg cgcgcgatct cgttcgcata gcacttggcg
      121 aggctcgact ccttgagcga gggcagtccc tggcccgagt tcaccgccgc ccggtggatc
      181 agcagccgcg cggcgtccac ccgcatcgcc atctcggcga gcttgagctg gaccgcctgg
      241 aactcgacca gcggcttgcc gaactggcgc cgctcctgga cgtaggcgag cgcgtcgtcg
      301 agcgcggcct gcgcctggcc gagcgccatg gtcgcattgc cgcagcgctc caggtcgaac
      361 gcctgcatca ggcgcgcgaa gccgccggcg tcgacgacca ggttgtcggc cggcacgcgc
      421 acctcgtcga gatggatgtc ggcgctgggc acgccgcgaa agcccatcag ggtctcgttc
      481 gggccgaaac tgacgcctgc gcggtccttc tccacataga ccgcgccgat gcccttggcg
      541 cccggctggt tggacaggcg gcagtagacc aggtagccgt gggcgtggcc gccgccgctg
      601 gtccagcgct tgtggccgct gaccacgatc tcatcgccct cgcgccgcgc gcgcgtggtg
      661 aggtcggtga gcgccgagcc cgcgtcgggt tcggacatcg acaccgccac caccacctcg
      721 ccccggcaca ccgccggcag cacgcgccgg cgcaaggagt cggagccgaa ggcctcgatc
      781 gccttgaccg gcccgacgca gctctcgaac accgggaagg cgaccgcgga gctgaccttg
      841 gcgaactcct cgaccaccag cagcgcctcg aggttgccga ggccgaggcc gccatatacg
      901 tgcgcgacgt tgatgccgag aaagccgagc tcggcgtagc gccggcgcac gccgtcgggc
      961 agcgggcggt tgtcgcgctc gagctcgcgc gccacgccct cgagctcgcc gcgggcgaac
     1021 ttccgcgccg cctcctggag cgcgcgctgc tcgtcgctga gcctgaagtc cacgccgatc
     1081 ctccccgaat cccgtcgcac cctagcccaa accgcgccgc agcgcgagcg gtcgcggttg
     1141 catgttgcgg ctgcgccggt caacattcgc gggcgagttc caccatgtgg aagggaggct
     1201 tacatgcacg cattcgccag gatcgcggtc gtcgtcttgt gcgccctgtc gttcgctgcg
     1261 gtcaccggcg ccgccagctt cgccgccgac gagccggcca acatcatcaa gtaccgccgc
     1321 aacgtgatga aggcgatcgg cgccaacatc gccaatatcg ccatggtggt gaagggcgag
     1381 gtcacctatt ccgccaacgt gccgttcaac gcgcgctcga tccgcgacgc cctcaacctg
     1441 gtcgacccct tattcccgcc gggcaccgac accggcgaca ccaacgccct gcccaagatc
     1501 tgggaggacg ccgccggctt caatgacgtg cacgacaagg cgcaagccgc cgccgacgcc
     1561 atggtccagg ccgccgagtc gggcgatgcc gccaacatcg ccgctgcgct cggcgacctc
     1621 ggcaaggcat gcggcaactg ccacaagccc taccgcgtgg attccagctc ggcggcgtgg
     1681 cggccatagg cgccgccgcc gggatcgggg tgggactggc cgcggcgggt gcctttgcgc
     1741 tcgccgccgc ggccgcggac gaggcggtcg cgcgcggcgc gtatctggcg cgcgcgggcg
     1801 gctgcttcgc ctgccacacc gacgtcaagg gcggcggcgc cccgctcgcc ggcggcgggc
     1861 ggatcgagac cccgttcggc accttcttcg gccccaatct caccccgcat cccgaccacg
     1921 ggctcgggcg ctgggccgag gccgacttcg tcgctgcgat gaccgagggc cgcgcccccg
     1981 acggcgcgcg ctactacccg gccttcccct acacatcgtt cagcgcaacc gcgcgcgccg
     2041 acctcgccga cctgtggtcg tacctgcgct cgcttgcgcc ggtggcgcgt gccaatacgc
     2101 cgcacgagct caagtggccg ttcggttggc ggttcctcaa ccgcatctgg cagtggctgt
     2161 tcttccgccc gcaaaccttc gcgccggacc ccgcgcacga tgcacactgg aaccgcggcg
     2221 cgtacctgtc cgaggccctg ctgcactgca aggagtgcca cagcccgcgc agccggttgg
     2281 gcgcactgaa gatctcgctc gcctatgccg gcaaccgcga cgggccgggc gataccacgg
     2341 tgcccaacat caccgccgac gccgagaccg gcatcggccg ctggagcgag atcgacgtcg
     2401 tgtggctgct caagaccggc ttcaagcccg acggcaacga cgttcagggg atcatggccg
     2461 agctgatcga gcacggctcg agccacctca gcgacgacga cctgacggcg gtcgccgcct
     2521 atatccgctc gctcgagccg atccgctacc aggtgcgcaa gccgcgcgcc gagagcggga
     2581 ccccgggcgg cgagttcaat tactagaacg gcggcgggca agctcccgtc agccctcacc
     2641 cggccgcttg cgcggccgac ctctccccga ggagaggtgg atcgcgccgg cgccggtgcg
     2701 ttccctctcc caccgggaga gggacagggt gaggagctgt tcacaatttc gtgatcggag
     2761 tttaaccgga tcgggcgccg gcatacgtcg gaggcgccgg ccatcccggc cgggctcgaa
     2821 ttccgcttcg caccgaggaa tggcaggcca tgaccgaact cgccctctgc cggcggcccg
     2881 cggcgctgtt gcttgcgctc gccgtcgtgg cagcgccgac gctcgccgac gcgcgcccgg
     2941 ttcccgacag cttcgccgac ctggtcgagc gcctgtcgcc cgccgtggtc gcggtctcga
     3001 ccgaggaagt ggtcgcgacc ggcggccagc tgcccttcca gttcccgcca ggctcgccgt
     3061 tcgaggagtt cttccggcgc tatttcggcg cccctccgga agaccagggc ggcgcgcccg
     3121 agcagcggcg gatgagcgcg ggctcgggct tcatcgtcga ttcggccggc tacatcgtga
     3181 ccaacaacca cgtcatcgcg gacgccgaca cgatcacggt cacactcgcc gatgagtccg
     3241 agttcgaggc gacgctgatc ggcagcgatc cctcgaccga cgtcgccctg atcaagatcg
     3301 acgccggcca cccgctcgcc acggtcgtct ggggcgactc ggaccaggta cgcgtcggcg
     3361 actggacgct cgccatcggc aatccgttcg ggctgtacgg ctcggtcacc gccggcatca
     3421 tctcggcgcg cgcgcggacc ttgaacgcgg gcccgttcga cgacttcctg cagaccgacg
     3481 cctcgatcaa ccccggcaac tcgggcgggc cgctgttcaa cctggccggc gaggtgatcg
     3541 gcatcaacac cgcgatcttc tcgccctcgg gcggcaacat cggcatcggc tttgccatcc
     3601 cctcggcgct ggccaagccc gtggtcgatc agatccgcga ccacgggcgt gcgatccgcg
     3661 gctggctcgg cgtgcagatc cagggcgtca ccaaggagct cgccgccggc ctcggcgtca
     3721 aggaggcgta cggcgcgctg gtggtcaagg tcagcgacga cagcccggcc gcgcgcgccg
     3781 gcctcaagac cggcgacctg atcgtcggct accgcggcca gcgggtggac gacccgcgcg
     3841 cgctcgcgcg catggtcgc
//
LOCUS       DASWHU010000040         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_752631, whole genome shotgun sequence.
ACCESSION   DASWHU010000040 DASWHU010000000
VERSION     DASWHU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752631"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VGB88_01355"
     CDS             <1..308
                     /locus_tag="VGB88_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646113.1"
                     /translation="LGQCHYALGNYQTAKEMFAERIRRNPSTDASRVMMAAACGMLGE
                     ADEAQRQWREVFRINPSYSLAQRRRVLPYREPGDFDRIVEGLRRAGIKTDAAGGSGR"
     gene            305..1477
                     /locus_tag="VGB88_01360"
     CDS             305..1477
                     /locus_tag="VGB88_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9646114.1"
                     /translation="MTAAALALPRTQRPALAAALALAAVLFGAAAMIGARNAALFVLA
                     LGIGVTLYHASFGFTGAWRRVFVAGDISGVSAQIVMLAAASLLFAPVLAHGGAFGREI
                     AGAVAPVSVSMAFGAFLFGIGMQLGGGCASGTLYTAGGASLRMMLVLAFFCLGTFLGS
                     LDLHRWQQLPGIGPVSLGTALGWEVALPLQLALLGAIYLALRRAGGRNVVPLWPRAGV
                     GRRELVHGPWPLLLGAGLLALFNWLTLLIAGHAWSITWGFSLWTAKIAAALGWEPLSS
                     PFWRGGFQQAALARPVLADTVSVMNVGILVGAFAAAALAGRIAPRLDYTPRQLLAAVI
                     GGLAMGYGARLAYGCNIGAFFSGVASASLHGWVWILAAIPGNAIGIRLRPFFRLSR"
     gene            complement(1461..2420)
                     /locus_tag="VGB88_01365"
     CDS             complement(1461..2420)
                     /locus_tag="VGB88_01365"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9646115.1"
                     /translation="MPLPPSLEGRLSLPAVAAPMFLVSGPELVIAASEAGVVGAFPSL
                     NARPAEEYVRWLGQIERALAASPGAAPFAVNLIVHRSNKRLEQDLAATVAHRVPIVIT
                     SVGHPGEVVARVHAYGGLVWHDVIHLHHAHKAAAAGVDGLILVAAGAGGHAGTLSPFA
                     FLRQVRAVFDRTIVLAGAITDGAGIAAARVLGADLAYLGTRFIASRESRAPEGYKRMI
                     VEASAADIIYTPAISGIPGNFLRQSLERAGLDPDNLPPRPEIDMDKEGGARRAWRDVW
                     SAGQGVGGIDDVAPAAALVARLGAEYRAALAAAGAGPAVSATA"
     gene            complement(2685..>3056)
                     /locus_tag="VGB88_01370"
     CDS             complement(2685..>3056)
                     /locus_tag="VGB88_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9646116.1"
                     /translation="PARGRELWRRSDSLPFYAGALATAAGLVFTGDLHGRALALDADT
                     GALLWAHQTGAAIAGSPISYLLDAVQYVLFPSGMGGGEAHSPGAARTERPRLTAYALD
                     RAAERRAGAGERLMEAPAALP"
BASE COUNT          362 a         1125 c         1129 g          440 t
ORIGIN      
        1 tgctcggcca gtgccactac gcgctcggca actatcagac cgccaaggag atgttcgccg
       61 aacgcattcg gcgcaatccg agcaccgatg ccagccgggt gatgatggcg gccgcgtgcg
      121 gcatgctcgg cgaggcggac gaggctcaac ggcaatggcg ggaggtattt cggatcaatc
      181 cgtcatactc tctggcccag cgtcgccggg tcctgccgta tcgcgagccg ggcgacttcg
      241 accggatcgt cgagggtttg cgccgcgccg gcatcaagac cgacgccgcc ggcggctcgg
      301 gccgatgacc gcggccgcac tcgccctgcc ccgcacccag cgcccggcgc tcgccgcggc
      361 gctggcactc gccgccgtgc tgttcggcgc agcggccatg atcggcgcgc gcaacgcggc
      421 gctgttcgtg ctcgcgctcg gcatcggcgt cacgctgtat cacgcgagct tcggcttcac
      481 cggcgcctgg cggcgggtgt tcgtcgcggg cgacatctcc ggcgtcagcg cccagatcgt
      541 gatgctcgcc gcggcgagcc tcttgttcgc gccggtgctc gcgcacggcg gcgccttcgg
      601 ccgcgagatc gcgggcgcgg tggcgccggt gagcgtgtcg atggcgttcg gcgcgttcct
      661 gttcggcatc ggcatgcagc tcggcggcgg ctgcgcctcg ggcacgctgt acacggccgg
      721 cggcgccagc ctgcgcatga tgctggtgct cgcgttcttc tgcctcggca cgttcctcgg
      781 cagcctcgac ctgcaccggt ggcagcagct gcccggaatc gggccggtgt cgctgggcac
      841 ggcgttgggc tgggaggtcg cgctgccgct ccagctcgcg ctgctcggcg cgatctatct
      901 ggcgctgcgc cgcgccggcg ggcgcaacgt cgtgccgttg tggccgcgcg cgggcgtcgg
      961 ccggcgcgag ctggtccacg ggccgtggcc gctgctgctc ggcgcggggc tgctggcgct
     1021 gttcaactgg ctgaccctct tgatcgcggg ccacgcctgg tcgatcacct gggggttctc
     1081 gctgtggacc gccaagatcg ccgcagcgct cggctgggag cccttgtcga gcccgttctg
     1141 gcggggcggc ttccagcagg cggcgcttgc ccggccggtg ctcgccgaca ccgtctcggt
     1201 gatgaacgtc ggcatcctgg tcggcgcgtt cgccgccgcg gcgctcgccg ggcggatcgc
     1261 gccccggctc gactacacgc cgcgccagct cctcgccgcg gtgatcggcg ggctggccat
     1321 gggctacggc gcgcgcctcg cctacggctg caacatcggc gcgttcttca gcggcgtcgc
     1381 ctcggccagc ctgcacggct gggtgtggat cctggctgcg atcccgggca acgccatcgg
     1441 catccggctc aggccgttct tcaggctgtc gcgctgaccg cggggccggc gccggcggcc
     1501 gccagcgccg cccggtactc ggcgccgagc cgcgccacca gggccgccgc cggtgccacg
     1561 tcgtcgatgc cgccgacgcc ctggccggcg ctccacacgt cgcgccaggc gcgccgggcg
     1621 ccgccctcct tgtccatgtc gatctcgggc ctgggcggca ggttgtcggg atcgaggccg
     1681 gcccgctcca ggctctggcg caggaagttg ccgggaatgc ccgagatcgc cggcgtgtag
     1741 atgatgtcgg cggcgctcgc ctcgacgatc atgcgcttgt agccctccgg cgcgcggctt
     1801 tcgcggctcg cgataaagcg ggtgccgaga taggcgaggt cggcgccgag cacgcgcgcg
     1861 gcggcgatgc cggcgccgtc ggtgatcgcg cccgcgagca cgatggtccg gtcgaacacc
     1921 gcgcgcacct ggcgcaggaa cgcgaacggg ctgagcgtgc cggcatggcc gcccgccccg
     1981 gccgcgacca ggatcaggcc gtcgaccccg gcggcggcgg ccttgtgcgc gtgatggaga
     2041 tggatcacgt cgtgccacac caggccgcca taggcgtgca cgcgcgcgac cacctcgccc
     2101 ggatggccga ccgaggtgat cacgatcggc acccggtgcg cgacggtcgc cgccaggtcc
     2161 tgctcgagcc gcttgttcga gcgatggacg atcaggttga ccgcaaaggg cgccgcgccc
     2221 gggcttgcgg cgagcgcgcg ctcgatttgc ccgagccagc gcacatactc ctcggccggg
     2281 cgcgcattga ggctcgggaa ggcgccgacc acccccgcct cggacgccgc gatcaccagc
     2341 tcggggcccg agaccaggaa catcggcgcc gcgaccgcgg gcaggctcag gcgtccctcg
     2401 agcgacggcg gcaacggcat cggcgaacct ttcgggacgg ggccggcggc gggcgcaaat
     2461 cctcgacgcg cctgtggacc gagtcaaggc cgcgcccccg actccctgat cgagatcaag
     2521 gcggcctcgc acggccatgg aatgatcgcc gcgatcgaga cgatcggatc gcccgatgac
     2581 aagcgttgat gcggcgtacc tgatctacgt catcgtggcg ttcgccgcgt tcatgggcgt
     2641 gctcgcctgg gcgatgcacc aggcgccggg cacgcgctag gcgctcaggg tagcgccgcc
     2701 ggcgcctcca tcaggcgctc acccgcgccc gcccgccgct cggccgcccg gtcgagggca
     2761 taggcggtca ggcgcggccg ctcggtgcgc gcggcgccgg ggctgtgcgc ctcgccgccg
     2821 cccattcccg aggggaacag cacgtactgg acggcgtcga gcaggtagct gatcgggctg
     2881 ccggcgatcg ccgccccggt ctggtgcgcc cacaacagcg cgccggtgtc cgcatcgagt
     2941 gccagcgcgc gcccgtgcag gtcgccggtg aacaccagcc cggccgcggt ggcgagcgca
     3001 ccggcgtaga acgggagcga atcgctccgc cgccatagct cgcgcccgcg cgcggg
//
LOCUS       DASWHU010000041        23185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3338542, whole genome shotgun sequence.
ACCESSION   DASWHU010000041 DASWHU010000000
VERSION     DASWHU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 23185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23185
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338542"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VGB88_01375"
     CDS             complement(<1..184)
                     /locus_tag="VGB88_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HEX9646117.1"
                     /translation="MPEGERRRRLAAILSADVVGYSRLMADDDRATLDTLQRYRAAIG
                     RIVDKHGGRVVNAPGDA"
     gene            complement(345..1040)
                     /locus_tag="VGB88_01380"
     CDS             complement(345..1040)
                     /locus_tag="VGB88_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9646118.1"
                     /translation="MIDLYFWPTPNGFKITIMLEEVALPYTVIPVDIGKGDQFKPEFL
                     KISPNNKIPALVDSDGPDRKPIALFESGAMLIYLADKTGQLLPTAPRARYQVLQWLMF
                     QMGTVGPMLGQTHHFRNYAPEKLDYAINRYTREAGRIYRVIDERVAERDYLAGDYSIA
                     DIATWPWILGERQGQNFDDYPNLSRWRDRIKERPAVIKGREVMKDTRPVGDPAKDKER
                     FEILFGDRQYARR"
     gene            1153..3300
                     /locus_tag="VGB88_01385"
     CDS             1153..3300
                     /locus_tag="VGB88_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX9646119.1"
                     /translation="MERSFTKEIRQLALGAGETFHGEGILAVTKALLQSGVSYVGGYQ
                     GSPISHLLDVMVQASDLLDELGVHLETPTGEAAAAAMLGASINYPMRGAVTWKSTVGT
                     NVASDALSNLASAGVKGGALIILGEDYGEGASIIQERSHAFAMKSSMCLVDPRPHLPT
                     IVNLVEKGFELSEASNMPVMLELRIRACHLFGRFECKDNRAPAISRRHTLAAPDFDYA
                     RICLPPATYAQEIHKVAHRLPAAVRFVAEHGLNEVLAGDLDEIGIITQGGLAHTVLRA
                     LAEAGLADAFARTRVPVYVMNVTYPLVPDEITAFCAGKRAVLMVEEGYPDYLEQAVNV
                     ALRRADLQTRVIGKDALPKVGEITAEVMARGLKAFLEDTKPAGVALADSRTPVDACDA
                     LKARAAALIGEPVPARPPSFCTGCPERPVFSAMKIVEKELGPTHVAADIGCHTFSTLA
                     PFNIGNTVLGYGLGLASSTGVAPAFAKRVISVMGDGGFWHNGLTTGVGSASFNRDDGV
                     LLIFKNGYSSATGWQVLPSSATDRTGRPSGMSIQKALRGLGVRWIRTVRTYDVARMVR
                     TLREALTTGAKGLKVIVADGECQLARQRRIRPQIRKRLDAGGRVVRTRYGVDDEVCTG
                     DHSCIRLSGCPSLTVKPNPDPLRTDPVATVIESCVGCGLCGEVAHAAVLCPSFYRLDV
                     IQNPNRLDRLLMRVRERVIGALGGRPAPDARAA"
     gene            3297..4847
                     /locus_tag="VGB88_01390"
     CDS             3297..4847
                     /locus_tag="VGB88_01390"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEX9646120.1"
                     /translation="MSDPAPTRPLTVLIAALGGEGGGVLASWLVGAATAAGLPVQATS
                     IPGVAQRTGATTYYVEVWPEPHSALGGREPVFGLYPAPGGVDVMVASELVEAGRALGN
                     GYVAPERTLLIASTHRVFAMAEKTAMADGRFDGARVAEAARALAARAVLADLDAIARA
                     AGAPINAVLFGAIAGAEVLPIPDAAWREAIRGEGKAVESNLAGFEAGLGLVRGAAAEV
                     PSDEAEAAPRTALPPVPARLQARLDAFGAPKLQEFATAGVRRLIDFQDTAYARRYLDR
                     VERVCAADPSPDKRIAAEAARQLALRMSFEDVIRVAQLKVRPERIARIRAEAGVAAGQ
                     PVRVVEVLKPGIEELCAILPGFVARPVLAWARRRNLSDRLNVGLKISSTSVGGFLLLK
                     ALAAMRPLRRLGHRFGEERRLIDDWLGAVGDACRIDAGLALEVADCARLIKGYGETHR
                     RGLANYQRIREAAIAPALAGRIAPEEAAELVRRAREAALADPDGRRLDAVLAAPVPGT
                     SETRAAAE"
     gene            4856..5269
                     /locus_tag="VGB88_01395"
     CDS             4856..5269
                     /locus_tag="VGB88_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9646121.1"
                     /translation="MAGEANRRLVEAFYERLWNRGELDAADELLAADFAFRGSLGYQA
                     HGPDGFRTYVGMVRAAFPDFHNALEETIAEGERIAARLTWTGTHRGPFLGIAPTGRRI
                     SYQGVGLFTVAGGRLASAWVVGDGYALLRQLGALG"
     gene            complement(5580..6479)
                     /locus_tag="VGB88_01400"
     CDS             complement(5580..6479)
                     /locus_tag="VGB88_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9646122.1"
                     /translation="MTGKDGEVQRVLVAGYGVMGRGIALSFARAGFETLVLSRDPARL
                     GLLPHGMRAVAEPPETPPDLVIESIIEQLAPKQALFKRLEAAYGSGDDGPILATNTSG
                     LALADIAAPLTARARFLAVHYMQPAETLPMVEVARLPETRADVLERTVRALARTGKES
                     IVLNRAVTGFLINRLQHAVLHEAYHLIETGVASVADVDNFARKLFGPRMCITGLIEQK
                     DLSGLDTHALAQQSIVPDLYHSPEPCRILQDKFEAGELGIKTGKGFYDWAGRDTAAFK
                     ADRQAKLDRLLAFLDELWADEST"
     gene            6538..7635
                     /locus_tag="VGB88_01405"
     CDS             6538..7635
                     /locus_tag="VGB88_01405"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEX9646123.1"
                     /translation="MRAFVYNAQPGRVVFGAGAWAKVPDEVDRLGVRRVLITTSRGNR
                     PRAEELAARLGARSAGVLDDARAHVPFEQAEDARATAAERRADGVVSVGGGTPIGLAK
                     AIAHALDIPVIAVPTTYSGSEMTGLSGITRDGVKRVIAAPAMLPRTVIYDPELTYDLP
                     AAATAATGMNAVAHCVEALYVPAANPVTSLIAEAGLAALVRGLPRAVAAPRGIEGRAE
                     ALYGACLAGMAVATAGIAIHHKLCHVLGGTYGLGHGEANAVVLPHAVAFNAPAAVGPI
                     ARVARAIGAAEPGLDDASAAARAAPFLFDFDTAIGAPTSLEALGLAHDALDEAARIAL
                     ELITDNPRPVDEAAVRALLEDAWHGRRPAAA"
     gene            7666..8376
                     /locus_tag="VGB88_01410"
     CDS             7666..8376
                     /locus_tag="VGB88_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX9646124.1"
                     /translation="MADDLVSAATLAALKTFDTPSVCNAIELVEPERRAYGFTTATLH
                     CVRPELGAMVGYARTVAVRASRPATADAEAVRATRLAYLDYVATGAKPTISVVHNLDH
                     GEEGFGSFWGEVNSAIHSALGCIGTVTNGSVRDIDALAPGFQVLAGSVGPSHGFVHVE
                     QFGDTATIAGMVVRSGDLVHADRHGAVVLPRGKADEVVEAAAKIARREAVVLKAARAK
                     GATLEAITRAMNESAKVS"
     gene            complement(8389..9966)
                     /locus_tag="VGB88_01415"
     CDS             complement(8389..9966)
                     /locus_tag="VGB88_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9646125.1"
                     /translation="MAPLRDMLSSKELIERTAISRSTLNNYISLGLLPKPVVRPATGK
                     DGRAPRIGYFPKQSLEALKRIAGLKQKGHSMAEIADMMATAGTAARAAARSETRAAAQ
                     RPAEEPPAAPPLPAAPASDAELGPLPSITDVRHPAYMVSNTFAVTWANRAAEREIFGR
                     PDGLPDDPGERSLFRLFSSARQRRDASWQAPLRFHVAIAKRRLSKAALLSTAVGGDAE
                     AAIELGRLYDEVEPAAPRPLFHAEVDLVTEPEGSRRWDLFATVFREGVLFAYVPRAEP
                     SYGLLNILSRRELAIRDLMLDLRPYLTPLAVLAAELRDAAKIRAELAADEYFELINQV
                     WAATAPLLRKYHGTAGQHAGDGFACFFFPQPDRHYIINALRCAHEANVLTRQLSHAWQ
                     ARKNWDNDLGLNVGIDEGEAWVGSCRTPAHLELTALGEAVERSARLSGFARDGSVWVT
                     KNLLGKLPARMRAGVRYGIRRRSGTGETMIVESSFARLSSLIDPGDARMGAFQDIAAL
                     PVTQVLDVRAEASDPDG"
     gene            10343..10729
                     /locus_tag="VGB88_01420"
     CDS             10343..10729
                     /locus_tag="VGB88_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9646126.1"
                     /translation="MMITKGFKRMLAEANAEIGAVSPVAAIEALSSGEAVFVDVRESA
                     EQATGTIPGAVAAPRGFLEFIADPEGPMHRPELVAGRPLIIYCASGGRSALAAKTLRD
                     MGIANVSNLVGGIGAWKQAGGPIEGA"
     gene            complement(11070..12224)
                     /locus_tag="VGB88_01425"
     CDS             complement(11070..12224)
                     /locus_tag="VGB88_01425"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEX9646127.1"
                     /translation="MAKHTGHASGGNEPPGPGLLLTPGPLTTDPRTRAAMMRDWGSRD
                     RDFIELTARVRRRLADLCNLGDDYACVPIQGSGTFAVEAMIATLVPAGGRLVVAVNGA
                     YGQRIATIARRLGRQVETADSPEHLPTDLARLAQVLDRAPRASHLALVHCETTSGVLN
                     PLADVAALARERNIRLLVDAMSSFGALELDGAALGLEAVAASANKCLEGVPGVAFVIA
                     RRDALAAAAGNCASLSLDLHDQWRALEANGQWRFTPPTHVIAALDCALDQLDGEGGPA
                     ARRVRYRQNCAALVAGMRRLGFETLLDDRDQAPIIVTFHLPADPGFSFERFYDALHRR
                     GVVIYPGKVTAAATFRIGCIGAIGQAAIETALAAVEDALAELGIASGAPA"
     gene            12422..13135
                     /locus_tag="VGB88_01430"
     CDS             12422..13135
                     /locus_tag="VGB88_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9646128.1"
                     /translation="MDDNPSAQLAGIRLLSHLGAEALKPVAQSCMWRRYRRNEQIVNR
                     ESESRDLFLVIKGSVEVANYSSTGREIAFATIPSGEYFGELSAIDDLPRSATVSAAED
                     CSIAIMPRMIFLELLRNDSRVATAVMRRLASIVRTCDDRIMDLSTLGAVQRVYVELLR
                     ICGPDPAVTNMWSVYPMPTQSDIAARASTTRETVARVLSQLTQDGLIQRKGRTLYLND
                     RDRLRELAEHLDTGAGPAR"
     gene            complement(13205..14278)
                     /locus_tag="VGB88_01435"
     CDS             complement(13205..14278)
                     /locus_tag="VGB88_01435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9646129.1"
                     /translation="MRSRGKLCQPPRRPKRADFLRIPTPYIDWHTTVFAPASKGLAMG
                     TSPSWVPASVSSLLPGFGPSGFETPILDALPASIAVLDPHGSVLFVNQAWSAFSGANG
                     GLEGEAAIGANFVEAGFWASDEDAPCDDIARACAGIRAVLNGTIDSFVLDYQCHTPIQ
                     EHWLELRVAPLAMATGIGAVLLQISITDRKRSADRAWRRANFDALTDLPNQSLLRDRL
                     TQAIALARRQGGGGAVLVVDIDHLREINRLYGHESGDEVLQQVAERLRGNVRDSDSVA
                     RIGDDEFAVLAPRLGQADALGTLKDKLDGALAAPYRWAGGQLRARAHIAVAVFPGAGE
                     TASEVLEAAAEALVKSKRDADNR"
     gene            14317..14748
                     /locus_tag="VGB88_01440"
     CDS             14317..14748
                     /locus_tag="VGB88_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646130.1"
                     /translation="MTDRRRPWLAALTGCAALVLSLAVAGGAADARPKRLDRDEVTAL
                     ITGNTVRGFNPSDDSTYTIFHSGNGEARAVLRNVNGQVSRSDGRWWVNDLGKLCVKWD
                     NFRWVNSCAVISRDEDAITFLDDNGRIVSFGEVVTGNPDDI"
     gene            complement(14788..15342)
                     /locus_tag="VGB88_01445"
     CDS             complement(14788..15342)
                     /locus_tag="VGB88_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9646131.1"
                     /translation="MAEIPPICDFGWKAPDFRLKGTDGAWHTLQELRGPKGTLVVFMC
                     NHCPYVKAILPRLIRDAKELAALGVTTVAISANDPGSYPDDSFDNMARIAREKQFPFP
                     YLYDESQGVARAYDAVCTPDFFGFNAALELQYRGRLDESKTNPVPNARRELYDAMRQV
                     AETGRGPRQQNASMGCSIKWRQAA"
     gene            15491..16306
                     /locus_tag="VGB88_01450"
     CDS             15491..16306
                     /locus_tag="VGB88_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646132.1"
                     /translation="MAGRIEDKVALVVGAGSIGPGWGNGKAAALLYAREGARLFAVDI
                     DGTAAEQTRAIIAAEGGRCESFVADAADARQVATAVDACRARFGRIDILHNNVGIAVV
                     GGVADTDEADWDRINAVNLKSVFLACRAVIPSMVAQGGGAIVNISSISGIRSVGVPLA
                     AYAATKAGILGLTMSIATSYARAGVRCNAVLPGLINTPLIHASLAEHYAGGDVERMIA
                     IRDAQVPRGRMGDAWDVAYASLFLASDEARFITGQALAVDGGQTAMCVTPPPG"
     gene            16424..17107
                     /locus_tag="VGB88_01455"
     CDS             16424..17107
                     /locus_tag="VGB88_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9646133.1"
                     /translation="MDQDIRPYPVAPGEPAPDFDLPMVSADGRATLGDYRGRSPLLIA
                     MFRRFECAFCRRLLAALKQTGEDLEPLGIETLAITGSPVRAARLYTKYRPPGLALASD
                     PMFAIHRRYGTPVCALTADGPSDWPRTANMSDAMKIVVNPNGELPEALPAPLAGEALD
                     KLDGFEEVVTDEPKAPPGNLALAGYFLIDREGIVRWRYIEAYDDPVTYGRPPSVDELM
                     VAARALARA"
     gene            17131..18156
                     /locus_tag="VGB88_01460"
     CDS             17131..18156
                     /locus_tag="VGB88_01460"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9646134.1"
                     /translation="MLRTTISGSLPKPSWLAEPGRLWAPWRPGDPDALAEAKHDAVRL
                     ALADQEEAGIDIVTDGEQSRQHFVHGFLEHIEGIDFAHKQRIGIRADRYRADCPTVVG
                     APRRLRPVHVEEVAFARAHTRRKLKFTLPGPMTIVDTLADAHFGDRARLAMAFARVLN
                     EEARDLVAAGADVIQFDEPAFNVYLDEVKGWGIDSLHAAIDGLDCTTAVHICYGYGIE
                     ANIRWKSTLGEAWLQYADTFPTLAASRIDQVSLECANSRVPMSVLDLLAGKDLLVGVV
                     DVATDRIESADEVAAVIRAALEHAPAERVFPCTNCGMVPLSRAVARGKLEAIGAGAAI
                     VRSELGG"
     gene            complement(18181..18867)
                     /locus_tag="VGB88_01465"
     CDS             complement(18181..18867)
                     /locus_tag="VGB88_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX9646135.1"
                     /translation="MRPRLLVVWGLLVVLVGAIVLHEGTGLFDPEPPAPTGRIPMFAF
                     TEPELGGVEVIYQGRQGALMRDPSGQWFLHDSSHSHSGTGTPGPQPAAGETHLADPGR
                     AAAITERLEVAARMLADRRVRPEGGLGDYGLANPQTMIAFYGRTPDGADYSRPLEVLY
                     VGDFLPGDYTYYAMRDGDQELSLIPRYFVAQLLATIYGEDQAPSLTPERQGTESDASA
                     PVESGPARRF"
     gene            complement(18879..20537)
                     /locus_tag="VGB88_01470"
     CDS             complement(18879..20537)
                     /locus_tag="VGB88_01470"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX9646136.1"
                     /translation="MPAICNGGGEVDRSFRAGLILLAGIASLASALAIQAILLETVLW
                     TVLLAVAGALLSAWGAFALRRELGAMMRRRRGEIALYTLGMIGVLVALGYFSARYPLR
                     FDMTEAGLYSLSEQTTTMLERLEQPVHIVFFHDPMMRETVELYELMAAQTDKVTVEFY
                     DPTLNPAQARMLGVQFAGTAILESEHRKLQINGDTETDIANGILRVSQGKTQVACFLD
                     GHQEADPFSMESHTHAEGAGPGHSHGLGTQYVLYERHGMSKARSGLEALNYEVKKISL
                     VMGEDALSDCSVLVVAGPKAALLPGEVEAIRAYLAAGGNAYFMLDPFVATGLDPVVGE
                     YGIVLDDTIVIDGASHYWTDASSPAVTEYNRHQITRDLPLTFYPGVRSLSPTPERVPG
                     TSVSPVVSSTGNSFAETSPDRAEFDEGIDLSGPATLMVVATRRPVSADDASAIQFGPQ
                     DQLAPVAANDDSVTGRSRIAVIGDSDFATNSFFHFLGNGNLFLNTVNYLAAQENLIGL
                     EPRSYELPRLSLTNRQVKGTFVLSVILVPALLALIGAGVWWKQR"
     gene            complement(20509..21216)
                     /locus_tag="VGB88_01475"
     CDS             complement(20509..21216)
                     /locus_tag="VGB88_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9646137.1"
                     /translation="MSSVLALYQKELWHYFRSPIAYFVVAVFMLGTGYFFTYNIFYTG
                     IASMAGTFQNMGILLLLVIPVVSMRLFSGEYSAGTMELLQTLPLRPWQVVLGKYLGAV
                     TILLLMTSATLIDLVPLYLFGDPETTTIAAGYIGFVLLGMACLAIGQFFSALTENQIV
                     AALITVSVLVAFWFVGHLQSFQTSYVLYALFGYLSFSGQFGAFVQGLVRSEAVLFYLI
                     VCAIALTLNASYLQWRR"
     gene            complement(21213..22169)
                     /locus_tag="VGB88_01480"
     CDS             complement(21213..22169)
                     /locus_tag="VGB88_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646138.1"
                     /translation="MIALESVTKRYGAKTIIHDMSFTVEKGEIVGFLGPNGAGKTTTI
                     RMIAGYTTATSGRVTVAGFDMATQNVEAAREIGYLPEHPPLYDILDVAAYLRFVAKVK
                     GVSRTEMAAELERVTAACHLEDVFRREIFKLSKGYRQRVGLAQAMLGKPQVLLLDEPT
                     IGLDPAQIHETREAIRSFGEGHAVLLSTHILPEVTMICRRVAIINHGRLLAIDTPEGL
                     RQAVERTNQVALQATAPADALRQELLSVDGVNAVQMDPAPGDAGRLSVECKVDARDGI
                     EAAIARVVAGRWELHGLERREPTLENVFLHYVHAAPVVEKAA"
     gene            complement(22254..23102)
                     /locus_tag="VGB88_01485"
     CDS             complement(22254..23102)
                     /locus_tag="VGB88_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9646139.1"
                     /translation="MKKLGIAAALLAGLCMAGASTASAQKLYWTASGQFGPFNIQGLI
                     PSYPEARDIKIPINMWVLDHPKGLIVYDTGNNVAISDGNCESHWTKGLCDFLKPGQTR
                     ADVIDKQLEKLGYKTSDVKIVITSHSHLDHIGNIEMFPNAIHVIQQIELYQAWWPEKF
                     QRGGAHVMADYDDAREFNYLALKGDYDLFGDGTVKILTTPGHTFGHQSVKLQLPETGT
                     VVLTQDAVWMEENMEGYPAGLNYSILDYTNSINRLKMIGDLENAKLWFGHSIKQYSEM
                     GGKWYK"
BASE COUNT         3441 a         8005 c         8240 g         3499 t
ORIGIN      
        1 gcgcgtcgcc gggcgcattc accacccgtc cgccatgctt gtcgacgatg cgcccgatgg
       61 cggcgcggta gcgctggagg gtgtcgaggg tggcgcggtc gtcgtcggcc atcagccgcg
      121 agtagccgac cacgtcggca ctcaggatcg ccgccaggcg tcggcgtctc tcgccctccg
      181 gcatcagccg cccttccagg acaatcgcga gtttacgggg tcggcgcaga cacccgaagt
      241 gtgccccaac gcggtgtccg tgtcatcttc gcgggcgcgc cgaccgcccc gccaacatgc
      301 ctgttcctgg ctgttccttg ccgtcgcggc cttggcgccg gtggtcagcg ccgcgcgtac
      361 tggcggtcgc cgaacaggat ctcgaaccgc tccttgtcct tggccgggtc gccgacgggc
      421 ctggtgtcct tcatcacctc gcggcccttg atcaccgccg ggcgctcctt gatccggtcg
      481 cgccagcgcg agaggttcgg atagtcgtcg aagttctggc cctggcgctc gcccaggatc
      541 cacggccagg tggcgatgtc ggcgatcgag tagtcgccgg cgagatagtc gcgctcggcg
      601 acccgctcgt cgatcacccg gtagatgcgc ccggcctcgc gggtgtagcg gttgatggcg
      661 tagtcgagct tctcgggcgc atagttgcgg aagtggtgcg tctgaccgag catcggcccg
      721 accgtgccca tctggaacat cagccactgc agcacctggt agcgcgcgcg cggcgccgtg
      781 ggcagcagct ggccggtctt gtcggcgaga tagatcagca tcgcgcccga ctcgaacagc
      841 gcgatcggct tgcggtcggg gccgtccgaa tccaccagcg ccgggatctt gttgttcggg
      901 ctgatcttga ggaactcggg cttgaactgg tcgcccttgc cgatgtcgac cgggatcacg
      961 gtatagggga gggcgacctc ctcgagcatg atggtgatct tgaagccgtt gggggtcggc
     1021 cagaagtaga gatcgatcac ctgaacctcc tgctttatat ccatgattgc cgggatcggt
     1081 ggaacccggc ttggcgcggc acgcgcggcg ggcgtatgct cgggccggcg caaggacgcg
     1141 gcaggataga cgatggaacg cagcttcacg aaggagattc gccagctcgc cctcggcgcc
     1201 ggcgagacct tccacggcga gggcatcctc gccgtcacca aggccctgtt gcagtcgggg
     1261 gtgagctatg tcggcggcta tcagggctcg ccgatctcgc acctgctcga cgtgatggtc
     1321 caggcctcgg acctcctcga cgagctcggc gtgcatctgg agacgccgac cggcgaggcg
     1381 gcggcggcgg cgatgctcgg cgcctcgatc aactacccga tgcgcggcgc ggtgacctgg
     1441 aagtcgacgg tcggcaccaa cgtcgcctcc gacgcgctgt cgaacctcgc ctcggccggg
     1501 gtcaagggcg gcgccctgat catcctcggc gaggactacg gcgagggcgc gagcatcatc
     1561 caggagcgca gccacgcctt cgccatgaag tcgtcgatgt gcctggtgga cccgcgcccg
     1621 catctgccga ccatcgtcaa cctggtcgag aagggcttcg agctgtcgga agcctcgaac
     1681 atgccggtga tgctggaatt gcgcatccgc gcctgccacc tgttcggccg cttcgaatgc
     1741 aaggacaacc gtgcgccggc aatctcgcgc cgccacacgc tcgccgcgcc cgacttcgac
     1801 tacgcgcgca tctgcctgcc gcccgcgacc tatgcccagg agatccacaa ggtcgcgcac
     1861 cggctgccgg cggcggtgcg cttcgttgcc gagcacgggc tcaacgaggt gctcgccggc
     1921 gacctcgacg agatcggcat catcacccag ggcgggcttg cgcacaccgt gctgcgcgcg
     1981 ctcgccgagg ccgggcttgc cgacgccttc gcacgcacgc gggtgccggt ctacgtcatg
     2041 aacgtcacct atccgctggt gcccgacgag atcaccgcgt tctgcgccgg caagcgcgcg
     2101 gtgctgatgg tcgaggaggg ctatcccgac tatctcgagc aggcggtcaa cgtcgcgctg
     2161 cggcgcgccg acctgcaaac ccgcgtgatc ggcaaggacg ccctgcccaa ggtcggcgag
     2221 atcaccgccg aggtgatggc gcgcgggctc aaggccttcc tcgaggacac caagccggcg
     2281 ggcgtcgcgc tcgcggacag ccggacgccg gtcgatgcgt gcgatgcgct gaaggcgcgc
     2341 gcggccgcgc tgattggcga gccggtgccg gcgcgcccgc cgagcttctg caccggctgc
     2401 cccgagcggc ccgtgttcag cgccatgaag atcgtcgaga aggagcttgg gccgacccac
     2461 gtcgccgccg acatcggctg ccacaccttc tcgaccttgg cgccgttcaa catcggcaac
     2521 accgtgctcg gctacgggct cgggctggcg agctcgaccg gggtcgcgcc cgccttcgcc
     2581 aagcgcgtga tcagcgtcat gggcgacggc ggcttctggc acaacgggtt gaccaccggg
     2641 gtcggcagcg cgagcttcaa ccgcgacgac ggcgtgctgc tgatcttcaa gaacggctac
     2701 agctcggcga ccggctggca ggtgctgccg tcgagcgcta ccgaccgcac cggccggccg
     2761 tcgggaatgt cgatccagaa ggcgcttcgc ggcctcggcg tgcgctggat acgcaccgtg
     2821 cgcacctacg acgtcgcgcg catggtgcgc acgctgcgcg aggcgctgac caccggcgcc
     2881 aagggcctca aggtgatcgt cgccgacggc gagtgccagc tcgcccgcca gcggcgcatc
     2941 cggccccaga tccgcaagcg gctcgacgcc ggcgggcgcg tggtgcgcac ccgttacggg
     3001 gtcgacgacg aggtctgcac cggcgaccat tcgtgcatcc ggctgtcggg ctgcccgtcg
     3061 ctgaccgtca agcccaaccc cgacccgctg cgcaccgacc cggtcgccac ggtgatcgag
     3121 tcctgcgtcg gctgcggcct gtgcggcgag gtggcgcatg ccgcagtgct gtgcccgtcg
     3181 ttctaccggc tcgacgtgat ccagaacccg aaccggctcg accggttgtt gatgcgggtg
     3241 cgcgagcggg tgatcggcgc gctcggcggc cggccggccc cggacgcgcg cgcggcatga
     3301 gcgatcccgc gccgacgcgg ccgctcacgg tgctgatcgc cgcgctcggc ggcgagggcg
     3361 gcggcgtgct ggcgagctgg ctggtcggcg ccgccacggc cgccgggctg ccggtgcagg
     3421 cgacctcgat cccgggcgtc gcgcagcgca ccggcgccac cacctattac gtcgaggtct
     3481 ggcccgagcc ccattccgcg ctcggcgggc gcgagccggt gttcggcctc tacccggcgc
     3541 cgggcggggt cgatgtgatg gtcgcgagcg agctggtcga ggccgggcgc gcgcttggca
     3601 acggctatgt cgcgcccgag cgcacgctgc tgatcgcctc cacccaccgc gtgttcgcga
     3661 tggccgagaa gacggcgatg gccgacggcc gcttcgatgg cgcccgggtg gccgaggccg
     3721 cacgcgcgct cgccgcccgc gcggtgctcg ccgacctcga cgcgattgcg cgcgccgccg
     3781 gcgcgccgat caacgcggtc ctgttcggcg cgatcgccgg cgccgaggtg ctgccgattc
     3841 cggacgcggc ctggcgcgag gcgatccggg gcgagggcaa ggcggtcgag tccaacctcg
     3901 ccggcttcga ggcgggtctc ggtttggtcc gcggcgcggc ggccgaggtg ccctcggacg
     3961 aggccgaggc cgcgccgcgc acggccctgc cgccggtgcc ggccaggctg caagcgcgcc
     4021 tcgatgcgtt cggcgcgccg aaattgcagg agttcgccac tgccggcgtc cgccggctga
     4081 tcgacttcca ggacaccgcc tacgcgcggc gctatctcga ccgcgtcgag cgcgtgtgcg
     4141 ctgccgaccc gagccccgac aagcgcatcg cggcggaggc cgcgcgccag ctcgcgctgc
     4201 gcatgagttt cgaggacgtg atccgcgtcg cccagctcaa ggtgcggccc gagcggattg
     4261 cgcgcatccg cgccgaggcg ggggtcgcgg cgggccagcc ggtgcgcgtg gtcgaggtgc
     4321 tcaagccggg catcgaggag ctgtgcgcga tcctgcccgg cttcgtcgcc aggcccgtgc
     4381 tcgcctgggc gcggcggcgc aacctgagcg accggctcaa tgtcggcctc aagatatcga
     4441 gcaccagcgt cggcgggttc ctgttgttga aggcgctcgc cgcgatgcgg ccgctgcgcc
     4501 ggctcggcca ccggttcgga gaggagcggc ggctgatcga cgactggctc ggcgcggtgg
     4561 gcgacgcgtg ccggatcgac gccggcctcg cgctcgaggt cgcggactgc gcgcggctga
     4621 tcaagggcta cggcgagacc catcgccgcg gcctcgccaa ctaccagcgc atccgcgagg
     4681 ccgcgatcgc gccggcgctc gccgggcgga tcgcgcctga ggaggcggcg gagctggtgc
     4741 ggcgcgcccg cgaggcggcg ctcgccgacc cggacggccg ccgcctcgac gccgtgctcg
     4801 ccgcgcctgt gcccggcaca tccgagacgc gggcggcggc cgagtagccg cacccatggc
     4861 cggcgaggcg aaccggcggc tggtcgaggc gttctacgag cggctgtgga accggggcga
     4921 gctcgacgcg gccgacgagt tgctcgccgc cgatttcgcc ttccgcggct cgctcggcta
     4981 ccaggcgcac ggtccggacg gcttccgcac ctatgtcggc atggtgcgcg cggcgttccc
     5041 cgacttccac aacgcgctcg aggagacgat cgccgagggc gagcgcatcg ccgcgcgcct
     5101 gacctggacc ggcacccacc gcgggccgtt cctcggcatc gcgccgaccg ggcggcgcat
     5161 ttcctaccag ggggtcggcc tgttcaccgt cgccggcggg cggctcgctt cggcatgggt
     5221 agtcggcgac ggctacgcgc tgctgcgcca gctcggcgcg ctcggatgac gggaccggac
     5281 ctcacgcgcc accaatgttc tcgtctgttc atgccgcacg cgtcaccccg gcgaggcgaa
     5341 gccgggaccc ggggtccata ctcttttgca accaaccggc cgctcgtgga ggcggcgaga
     5401 tggaccccgg ctcgcggcgc ttcgcgcctt ggccggggtg acggccgacg gatgatcggg
     5461 tctcaaatca tcccgcttaa gccgctggat tccggctctc cgctccgctc cggccggaat
     5521 gacggagacg ggtggtccgg cagcatcata ataactagct gaataatggt cggatcgttt
     5581 catgtgctct cgtccgccca cagctcgtcg aggaaggcga gcaggcggtc gagcttggcc
     5641 tggcggtcgg ccttgaaggc ggccgtgtcg cgcccggccc agtcgtagaa gcccttgccg
     5701 gtcttgatgc cgagctcgcc ggcctcgaac ttgtcctgga ggatgcggca cggctcgggc
     5761 gagtggtaca ggtcggggac gatgctctgc tgggcgagcg cgtgggtgtc gagcccgctc
     5821 aggtccttct gctcgatcag gccggtgatg cacatgcgcg ggccgaacag cttgcgcgcg
     5881 aagttgtcga cgtcggcgac cgaggccacc ccggtctcga tcaggtgata ggcctcgtgc
     5941 aggaccgcgt gctgcaggcg gttgatcagg aagccggtca ccgctcggtt gagcacgatc
     6001 gactccttgc cggtgcgggc gagcgcgcgc acggtgcgct cgagcacgtc ggcgcgggtc
     6061 tcgggaaggc gcgccacctc gaccatcggc agggtctcgg ccggctgcat gtagtggacg
     6121 gcgaggaagc gggcgcgggc ggtgagcggc gcggcgatgt cggcgagcgc gaggcccgag
     6181 gtgttggtgg cgaggatcgg cccgtcgtcg cccgagccgt aggcagcctc gaggcgcttg
     6241 aacagcgcct gcttcggcgc gagctgctcg atgatcgatt cgatcaccag gtcgggcggc
     6301 gtctccggcg gctcggcgac ggcgcgcatg ccgtgaggga gcaagccgag ccgggccggg
     6361 tcgcggctca gcaccaaggt ctcgaacccg gcgcgggcga agctgagcgc gatgccccgg
     6421 cccatcacgc cgtagcccgc caccagcacc cgctggacct cgccgtcctt gcctgtcatc
     6481 gcactccccc ctttgcgctc cctcgccggg cgctatgatg ccccggggag gatcagcatg
     6541 cgcgcgttcg tttacaacgc ccagcccggg cgggtggtgt tcggcgccgg cgcctgggcc
     6601 aaggtgccgg acgaggtcga ccggctgggc gtccgccggg tgctgatcac gaccagccgc
     6661 ggcaaccgcc cgcgcgcgga ggagctggcg gcgcggctcg gcgcgcgctc ggccggcgtg
     6721 ctcgacgacg cccgcgccca cgtgccgttc gagcaggccg aggacgcgcg cgcgaccgcg
     6781 gccgagcgtc gcgccgacgg cgtcgtctcg gtcggcggcg gcaccccgat cgggctcgcc
     6841 aaggcgatcg cgcacgcgct cgacatcccg gtgatcgccg tgccgaccac ctattcgggc
     6901 tcggagatga ccgggctctc cggcatcacc cgtgacggcg tcaagcgcgt gatcgccgcg
     6961 ccggcgatgc tgccgcgcac tgtgatctac gacccggagc tgacctacga cctgcccgcg
     7021 gcggcgaccg ccgccaccgg catgaacgcg gtcgcgcact gcgtcgaggc gctgtacgtg
     7081 ccggccgcca acccggtcac ctcgctgatc gccgaggccg gcctcgccgc ccttgtgcgc
     7141 ggcctgccgc gcgcggtcgc cgcgccgcgc ggcatcgagg gccgcgccga ggcgctgtac
     7201 ggcgcctgcc tcgccggcat ggcggtcgcc accgccggca tcgccatcca ccacaagctc
     7261 tgccacgtac tcggcggcac ctacggcctt ggccacggcg aggccaacgc ggtggtcctg
     7321 ccgcacgcgg tcgcgttcaa cgcgcctgcc gcggtgggcc cgatcgcccg cgtcgcgcgc
     7381 gccatcggcg cggcggagcc cggcctcgac gatgcctcgg ccgcggcccg ggcggcgccg
     7441 ttcctgttcg acttcgacac cgcgatcggc gcgccgacca gcctcgaggc gctcggcctt
     7501 gcccacgacg cgctcgacga ggcggcgcgg atcgcgctcg agctgatcac cgacaacccg
     7561 cgcccggtcg atgaggcggc ggtgcgcgcc ctgctcgagg acgcctggca cggccgccgt
     7621 cccgccgccg cctgaatcgc aacgcaaaca aggagacgtc ccgccatggc cgacgacctg
     7681 gtgtccgcgg caacgcttgc cgcgctcaag accttcgaca cgccgagcgt gtgcaacgcc
     7741 atcgagctgg tggagcccga gcgccgggcc tatggcttca ccacggccac gctgcactgc
     7801 gtgcggcctg agctcggcgc catggtcggc tatgcgcgca cggtggcggt gcgcgcctcc
     7861 cgcccggcga cggcggacgc ggaggcggtc cgcgccacgc ggctcgccta tctcgactac
     7921 gtcgccacgg gcgccaagcc gacgatcagc gtggtccaca acctcgacca cggcgaggag
     7981 ggcttcggat cgttctgggg cgaggtcaac agcgccatcc acagcgcgct cggctgcatc
     8041 ggcacggtca ccaacggcag cgtccgcgac atcgatgcgc tcgccccggg cttccaggtg
     8101 ctggccggct cggtcgggcc gagccacggc ttcgtccacg tcgagcagtt cggcgatacc
     8161 gccaccatcg ccggcatggt ggtgcgctcg ggcgacctgg tccacgccga ccgccacggc
     8221 gcggtggtgc tgccgcgcgg caaggcggac gaggtggtcg aggcggcggc gaagatcgcc
     8281 cggcgcgagg cggtggtgct gaaagccgcg cgcgccaagg gcgcaaccct ggaggcgatc
     8341 acgcgggcga tgaacgagtc ggcgaaggtg agctgagcgc gtgagctttc agccgtcggg
     8401 gtcgctcgcc tccgcccgga cgtcgaggac ctgagtcacc ggcagggccg cgatgtcctg
     8461 gaacgcgccc atgcgggcat cgcccggatc gatcaggcta gagaggcgcg cgaagctcga
     8521 ctcgacgatc atcgtctcgc cggtcccgga ccggcgccgg atgccgtagc gcacgcccgc
     8581 gcgcatccgc gccggcagct tgccgagcag gttcttggtc acccacaccg agccgtcgcg
     8641 ggcgaagcca ctcagccgcg cgctgcgctc gaccgcctcg ccgagcgcgg tgagctctag
     8701 gtgcgcgggc gtgcggcaac tgccgaccca cgcctcgccc tcgtcgatgc cgacgttgag
     8761 accgaggtcg ttgtcccagt tcttgcgcgc ctgccaggcg tggctcagtt gacgcgtcag
     8821 cacgttcgcc tcgtgcgcgc agcgcagcgc gttgatgatg tagtggcggt cgggctgggg
     8881 aaagaagaaa catgcgaagc cgtcgccggc gtgctggccg gcggtgccgt gatacttgcg
     8941 cagcagcggc gcggtcgccg cccagacctg gttgatcagc tcgaagtact cgtccgccgc
     9001 cagctcggcg cggatcttgg ccgcgtccct gagctcggcg gccagcaccg cgagcggcgt
     9061 cagatatggc ctgagatcga gcatcaggtc gcggatcgcc agctcgcggc gagacaggat
     9121 gttgagcagg ccgtaactcg gctcggcccg cggcacatag gcgaacagca cgccctcgcg
     9181 gaacaccgtg gcgaacaggt cccagcggcg gctgccctcg ggctcggtga ccagatcgac
     9241 ctcggcgtgg aacaagggcc gcggcgcggc cggctcgacc tcgtcgtaca gtcggccgag
     9301 ctcgatcgcc gcctcggcgt cgccgcccac cgcggtcgaa agcaacgctg ccttggacag
     9361 ccgccttttg gcgattgcga catggaagcg gagtggcgcc tgccaactgg cgtcccggcg
     9421 ttggcgggcg cttgagaaca gcctgaacag gctgcgctcg cccggatcgt cgggcaggcc
     9481 gtcgggcctt ccgaaaatct cgcgctccgc ggcccggttg gcccaggtga ccgcgaacgt
     9541 gttgctgacc atgtaggccg ggtggcggac atcggtgatg ctcggcagcg ggcccagctc
     9601 ggcatcgctc gccggcgcgg ccggcagggg cggcgccgcg ggcggttcct cggcggggcg
     9661 ctgcgcggct gctcgggtct cgctccgcgc agcggcgcgg gcggcggtgc ccgcagtcgc
     9721 catcatgtcg gcgatctcgg ccatgctgtg gcccttctgc ttgaggccag cgatgcgctt
     9781 gagcgcctcc agcgactgct tggggaagta gcctatgcgc ggcgcgcggc cgtccttgcc
     9841 ggtcgccggc cggaccaccg gcttgggcag caggccgagc gagatgtaat tgttcaaggt
     9901 cgagcgcgag atggcggtgc gctcgatcag ttccttgctc gacagcatgt cccgcaaagg
     9961 cgccatggct tggggcggag ccggcccgtt cggcgacgcg gactcgcgcc gcttattgtc
    10021 caaaaacgta gacgcaaact gtcagggaag caacgattcg cggcctttgg catcaggttg
    10081 ctcagatatg ttgccggaat gtgtccaagc tcctggcaca atcgccggca gggcgcgcaa
    10141 gccggcctgc ggcaaaagga ccgctcgcga aacgatcggg aaaggccatc gccttgcgtg
    10201 cgcgcccccg ccgtccgccg gcggcgcgcc ggcgccatcg ccgggatgga cgcggggcgt
    10261 cgcgatgggc taactcggga cgcgcgccgc cggtcgggcc gcggcggctc gcaccatctg
    10321 gatcgcggtt cgcggagaga cgatgatgat taccaagggc ttcaagagga tgttggccga
    10381 ggccaatgcc gagatcggcg cggtctcgcc ggtcgcggcg atcgaggcgc tgtcgagcgg
    10441 cgaggccgtg ttcgtcgacg tgcgcgaatc ggcagagcag gcgaccggaa ccatccccgg
    10501 cgcagtggcc gcgccgcgcg gcttcctgga gttcatcgcc gacccggaag gcccgatgca
    10561 ccggcccgag ctggtcgcgg gccggccgct gatcatctat tgcgccagcg gcggccgctc
    10621 ggccctggcg gcgaaaacgc tgcgcgacat gggcatcgcc aacgtcagca atctggtcgg
    10681 cggaatcggc gcctggaagc aggcgggcgg cccgatcgag ggcgcgtgac cgcgcccgca
    10741 aagcggcggc gatgcgcggt gcaaaaacaa acccggcgcc ggctgaaaat cctagccggc
    10801 gccgggcggg gtaatcgcgg aaccggtgag gagcggggaa tgcaccggtt ccgctttcgg
    10861 cctaataatt taggccgagc tgtggttctt tcggcggctc gccacccggg ttccgacttt
    10921 gcagccgcgt ccgcgggcgg agggtcgcca gggcccaaca gggcacgtcc tagtgtcatt
    10981 tgggggaaag ccggcgcgcc gacagtgact gccgtcacag ttgctgcgga acggcgccgc
    11041 ggtcgcctgt cgccgtggcg gcgggcccct caggccgggg cgccggaggc gatgccgagc
    11101 tcggcgagcg cgtcctcgac cgcggccagc gcggtctcga tcgcggcctg accgatcgcc
    11161 ccgatgcagc cgatgcgaaa cgtcgccgcc gcggtcacct tgccgggata gatcaccacg
    11221 ccgcggcggt ggagcgcgtc gtagaagcgc tcgaagctga agccggggtc cgccggcaga
    11281 tggaaggtga cgatgatcgg cgcctggtcg cggtcgtcga gcaaggtctc gaagccgagc
    11341 cgcctcatgc ccgcgaccag ggctgcgcaa ttctgccgat agcgcacccg gcgggccgcc
    11401 ggcccgcctt cgccgtcgag ctggtcgagg gcgcaatcga gggccgcgat cacgtgggtg
    11461 ggcggggtga accgccactg gccgttggct tccagcgccc gccactggtc gtggaggtcc
    11521 aggctgagcg aggcgcagtt gccggcggcg gcggcgagcg cgtcccggcg cgcgatcacg
    11581 aacgccaccc cgggcacccc ttccaggcac ttgttggccg acgccgcgac cgcctccagg
    11641 ccgagtgccg cgccgtcgag ctcgagcgcg ccgaacgagc tcatggcgtc gaccagcagg
    11701 cgtatgttgc gctctcgcgc cagggcggca acgtcggcga gcgggttgag cacgcccgag
    11761 gtggtctcgc aatggaccag ggcgagatgg cttgcgcggg gcgcccgatc gagcacctgc
    11821 gccaggcgcg ccaggtcggt cggcaggtgc tccgggctgt cggccgtctc gacctgccgg
    11881 ccgaggcggc gggcgatggt cgcgatgcgc tggccgtagg cgccgttgac cgcgaccacc
    11941 aggcggccgc cggcggggac cagggtggcg atcatcgcct cgaccgcgaa cgtgccgctg
    12001 ccctggatcg gtacgcaggc gtagtcatcg ccaaggttgc acagatccgc caggcgccgg
    12061 cgcacgcgcg cggtcagctc gatgaagtcg cggtcgcgcg aaccccagtc gcgcatcatt
    12121 gccgcgcgcg ttcgcgggtc ggtggtcagc ggccccgggg tcagcagcaa tcccggcccc
    12181 ggcggctcgt taccgccgct cgcgtgaccc gtgtgtttcg ccatcgctgt tcgcctccgt
    12241 tgcgactttc tatcgccaag ctgcgggccg cacaacgccg gccgccgagc gcgctgccgg
    12301 ggcagtggac gcgccccgcg ctccgtctac attaccttgc agggctggtg tgtaagcgtc
    12361 gtgggagggg ccggacgtgt cggaccgggc gcgccatcgg ttgagccgga gcgaaccgac
    12421 aatggacgac aatccgtcgg cgcagttggc cgggatcagg ctgttgtcgc atttgggcgc
    12481 cgaggcgctc aagccggtgg cccaaagctg catgtggcgc cggtaccggc gcaacgagca
    12541 gatcgtcaat cgcgaaagcg agagccgcga cctgttcctg gtgatcaagg gatcggtcga
    12601 ggtcgccaac tactcgtcca ccggccgcga gatcgcgttc gcgaccattc cctcgggcga
    12661 gtatttcggc gagctgtcgg cgatcgacga cctgccccgg tcggcgacgg tgtcggccgc
    12721 cgaggactgc tcgatcgcca tcatgccgcg gatgatcttc ctcgagctgc tgcggaacga
    12781 ctcgagggtg gcaacggcgg tgatgaggcg cctggccagc atcgtacgca cctgcgacga
    12841 ccggatcatg gacctgagca ccctcggcgc cgtccagcgc gtctatgtcg agttgctgcg
    12901 catctgcggg cccgacccgg cggtgaccaa catgtggtcg gtctatccga tgccgaccca
    12961 gtccgacatc gccgcgcgcg ccagcacgac gcgcgagacg gtggcgcggg tgctcagcca
    13021 gctgacccag gacgggctga tccagcgcaa ggggcggacg ctctatctca acgaccgcga
    13081 ccgcctgagg gagctggccg agcatctgga caccggcgca gggccggcgc gctgaggccg
    13141 gccggtcgaa gcctatcagg cgggcagtcg tcggggtcgc gatcgcgcgg cgagatgcat
    13201 gcggctaccg gttgtcggcg tcgcgtttcg acttgaccaa cgcctcggcc gcggcctcga
    13261 gcacctccga cgcggtctcg ccggcgccgg gaaagacggc gaccgcgata tgggcgcgcg
    13321 cgcgcagctg cccgcccgcc caccggtagg gcgcggcgag cgccccgtcc agcttgtcct
    13381 tgagcgtgcc gagcgcgtcc gcctggccga gccggggcgc cagcaccgcg aactcgtcgt
    13441 cgccgatccg ggcgacggaa tccgaatcgc gcacgttgcc gcgcaggcgc tcggcgacct
    13501 gctgcagcac ctcgtcgccg ctctcgtggc cgtagagccg gttgatctcg cgcaagtggt
    13561 cgatgtcgac gaccagcacc gctccgccgc cgccctggcg gcgcgcgagc gcaatcgcct
    13621 gggtcagccg gtcgcgcagc agcgactggt tcggcagatc cgtcagcgcg tcgaagttcg
    13681 cccgccgcca ggcgcgatcc gccgaacgct tgcgatcggt gatcgagatc tgcagcaaca
    13741 ccgcgccgat gcccgtcgcc atcgccagcg gcgccacgcg gagctcgagc caatgctcct
    13801 ggatcggagt atggcactga taatcaagca caaatgagtc gatggtgccg ttgagcaccg
    13861 cccggatgcc ggcgcaggca cgggcgatgt cgtcgcacgg cgcgtcctcg tccgaagccc
    13921 agaagccggc ctcgacgaag ttcgcgccaa ttgcggcctc gccctcgaga ccgccgtttg
    13981 cgcccgagaa cgcggaccac gcctggttga cgaacagcac cgagccgtga ggatcgagca
    14041 ccgcgatgct cgccggcagc gcgtcgagaa tcggcgtctc gaaaccggac gggccgaaac
    14101 cgggaagcag cgacgacacg gaagcgggca cccacgatgg cgacgtcccc atcgccaacc
    14161 ctttcgaagc cggggcaaaa acggtcgtgt gccagtcaat atagggcgtg gggattcgaa
    14221 ggaaatcggc ccgcttgggg cggcggggcg gttgacagag cttgccccgg ctcctcaaat
    14281 catcgctctg aggaggcggc aatgaggtgc gaagctttga cggacaggcg gcggccttgg
    14341 cttgcggcgc tcaccggctg cgcggcgctg gtgctgtcgc tcgccgttgc cggcggggcc
    14401 gccgatgcca ggccgaagcg cctcgaccgc gacgaggtca cggcgctgat caccggcaac
    14461 accgtgcgcg gcttcaatcc cagcgacgac agcacctaca ccatattcca ctcgggcaac
    14521 ggcgaggccc gggccgtgct gcgcaacgtc aacggccaag tctcgcgctc ggacgggcgc
    14581 tggtgggtga acgatctcgg caagctgtgc gtcaaatggg acaacttccg ctgggtgaac
    14641 tcgtgcgcgg tgatcagccg cgacgaggac gcgatcacct tcctcgacga caacggccgc
    14701 atcgtctcat tcggcgaggt cgtcaccggc aatccggacg acatttgagc gggcgggccg
    14761 gcgcggccac gccggcaggc ccgccgctca ggccgcctgc cgccacttga tcgagcagcc
    14821 catgcttgcg ttctgctggc gcgggcctcg gccggtttcg gcgacctggc gcatcgcgtc
    14881 atacagctcg cggcgcgcgt tcggcaccgg gttggtcttc gactcgtcca gccggccgcg
    14941 atattgcagc tcgagcgcgg cgttgaagcc gaagaagtcg ggcgtgcaca ccgcgtcgta
    15001 ggcgcgggcg acgccctggc tctcgtcgta caggtagggg aacgggaact gcttctcgcg
    15061 cgcgatccgc gccatgttgt cgaagctgtc gtccgggtac gacccgggat cgttggcgct
    15121 gatcgcgacg gtcgtcacgc cgagcgcggc aagctccttc gcgtcccgga tcaggcgcgg
    15181 caggatcgcc ttcacgtacg ggcaatggtt gcacatgaac accaccaggg tgcccttcgg
    15241 cccgcgcagc tcctggagcg tatgccacgc gccgtcggtg cccttgaggc ggaagtccgg
    15301 cgccttccag ccaaaatcgc agatcggggg aatctcggcc atcggtcatg ttcctggtcg
    15361 ggtttcgggg catgaggcga cggtggcaag gccccgccgc ctgcgctacc ggttatacga
    15421 aatccggcgg cgcgaggcca tgtgttggcg cacgcgccgg ccggccccgg tcgcaggcag
    15481 ggaggcgagc atggcgggac gcatcgagga caaggtggcg ctggtcgtcg gagccggctc
    15541 gatcgggcct ggctggggca acggcaaggc ggcggcgttg ctgtatgcgc gcgagggcgc
    15601 gcgtctgttc gcggtcgaca tcgacggcac ggcggccgag cagacgcggg cgatcatcgc
    15661 cgccgagggc ggccgctgtg agagcttcgt cgccgacgcc gcggacgcgc gccaggtcgc
    15721 gacggcggtc gatgcctgcc gcgcccgctt cgggcgcatc gacatcctcc acaacaacgt
    15781 cggcatcgcc gtggtcggcg gcgtcgccga caccgacgag gcggactggg accggatcaa
    15841 tgcggtcaac ctgaagagcg tgttcctggc ctgccgggcg gtgatcccgt cgatggtcgc
    15901 ccagggcggc ggcgcgatcg tcaacatctc gtcgatctcg ggcatccgct cggtcggcgt
    15961 gccgctcgcc gcctacgccg ccaccaaggc gggcattctc gggctgacca tgagcatcgc
    16021 cacgagttac gcgcgcgccg gcgtgcgctg caacgcggtg ctgccgggcc tgatcaacac
    16081 gccgctgatc cacgcctcgc tggccgagca ctatgccggc ggcgatgttg agcggatgat
    16141 cgcgatccgc gatgcgcagg tgccgcgcgg acgcatgggg gacgcctggg atgtcgccta
    16201 tgcgtcgctg ttcctggcct ccgacgaggc ccggttcatc accggccagg cgctcgccgt
    16261 cgacggcggc cagaccgcca tgtgcgtgac cccgccgccg ggctgagccc agggcgctgc
    16321 caaagaggtg gactcggcgc gtgcgcgcgg ccgtgatagc ttacggcttg aggcgccggg
    16381 cggcccaagc ggtggccgtc ggccgatgcg cggaggcgta acgatggacc aggacattcg
    16441 cccttatccg gtggcgccgg gcgagccggc gccggacttc gacctgccga tggtctcggc
    16501 ggacgggcgc gcgacgctcg gcgactaccg cggccggtcg ccgctgctga tcgccatgtt
    16561 ccgccgcttc gaatgcgcgt tctgccggcg cctgttggcg gcgctgaagc aaaccgggga
    16621 agacctggag ccgctgggga tcgagacgct cgccatcacc ggctcgccgg tgcgggcggc
    16681 ccggctgtac accaagtacc ggccgccggg actggccttg gcctcggatc cgatgttcgc
    16741 catccaccgc cgctatggca cgccggtgtg cgccctgacc gcggacgggc cgtccgactg
    16801 gccgcggacg gcgaacatgt ccgacgcgat gaagatcgtg gtcaatccca acggcgagct
    16861 gcccgaggcg ctgccggcgc cgctggcggg agaggcgctc gacaagctcg acgggttcga
    16921 ggaagtggtc accgacgagc ccaaggcgcc gcccggcaac ctggccctgg ccggctactt
    16981 cctgatcgac cgcgagggaa tcgtgcgctg gcgctacatc gaggcgtatg acgatcccgt
    17041 cacctacggc cggccgccga gcgtggacga gctcatggtc gcggcgcgcg cgctcgcccg
    17101 agcctgaccg accgcgtcgt cggccacggc atgttgcgga ccacgatcag cggcagcctg
    17161 cccaagccga gctggctcgc cgagccgggc cggctgtggg cgccgtggcg gccgggcgat
    17221 cctgacgcgc tcgccgaggc caagcacgac gccgtgcgcc ttgcgctcgc cgaccaggag
    17281 gaggccggca tcgacatcgt caccgacggc gagcagtcgc gccagcactt cgtccacggc
    17341 tttctcgagc acatcgaggg catcgacttc gcccacaagc agcgcatcgg catccgcgcc
    17401 gaccgctacc gggccgactg cccgacggtg gtcggcgcgc cccgccgcct gcgcccggtg
    17461 cacgtcgagg aggtggcctt tgcccgcgcc cacacgcggc gcaagctcaa gttcaccttg
    17521 cccgggccga tgaccatcgt cgacacgctc gccgatgcgc atttcggcga ccgggcgagg
    17581 ctcgccatgg cgttcgcgcg ggtgctcaac gaagaggcgc gcgacctggt cgccgccggc
    17641 gccgacgtga tccagttcga cgagccggcg ttcaacgtct atctcgacga ggtcaagggc
    17701 tggggcatcg acagcctgca cgccgcgatc gacgggctgg actgcacgac cgcggtccac
    17761 atctgctacg gctacggcat cgaggcgaac atccgctgga aatcgaccct cggcgaggca
    17821 tggctgcaat atgccgacac cttcccgacg ctggccgcaa gccggatcga ccaggtgtcg
    17881 ctcgaatgcg ccaactcgcg ggtgccgatg tcggtgctcg acctgctcgc cggcaaggac
    17941 ctgctggtcg gcgtggtcga cgtcgccacc gaccgcatcg agagtgccga cgaggtcgcc
    18001 gcggtgatcc gcgctgcgct cgagcacgcg ccggcggaac gagttttccc gtgcaccaac
    18061 tgcggcatgg tgccgctgtc gcgcgcggtg gccaggggca agctggaagc gatcggcgcc
    18121 ggcgccgcga tcgtgagaag cgagctcggc ggctgaagcc cggcgggtgc gcgcgcctcg
    18181 ctagaaccgt cgtgcaggac ctgactcgac cggcgccgac gcatcgctct ccgtgccttg
    18241 gcgctccggc gtgagcgacg gcgcctggtc ctcgccgtag atcgtcgcca agagctgtgc
    18301 gacgaagtag cgcgggatca gcgacagctc ctgatcgccg tcgcgcatgg cgtaatacgt
    18361 gtagtcgccg ggcaagaagt cgcccacgta aagcacctcc agcggccgcg aatagtcggc
    18421 accgtcgggc gtgcgcccgt agaacgcgat catggtctgc gggttggcga gtccataatc
    18481 gccgagtccg ccttcgggcc gcacgcgccg gtcggcgagc atgcgtgcgg ctacctcgag
    18541 tcgctcggtg atcgccgcgg ctcggccagg atcggcaaga tgtgtctcgc cggcggcggg
    18601 ttgtgggccg ggcgtccctg tgccgctgtg gctgtgactc gagtcgtgca ggaaccactg
    18661 gccgctggga tcgcgcatca gggcgccctg gcggccctga tagatcacct cgaccccgcc
    18721 cagctcgggc tcggtaaagg cgaacatcgg gatgcgcccg gtcggggccg gcggttcggg
    18781 gtcgaacagg ccggtgcctt cgtgcaggac gatcgccccg accaacacca ccagcagccc
    18841 ccagacgacc agcagccgcg gacgcatccc gcccacgctc agcgctgctt ccaccacacg
    18901 ccggcgccga tcaaggccag taacgccgga accaggatca cggacaggac gaaggtgccc
    18961 ttcacctggc ggttggtgag actgaggcgg ggcagttcgt agctgcgtgg ctcgaggccg
    19021 atcaggttct cctgggcggc aagataattg accgtgttca ggaacaggtt gccgttgccg
    19081 aggaagtgga agaaagagtt ggtggcgaag tccgagtcgc cgatcaccgc gatccgcgac
    19141 ctgccggtga cggaatcatc gttcgccgcg accggcgcaa gctggtcctg cgggccgaac
    19201 tggatcgccg acgcatcgtc ggcgctcacc ggtcggcggg tggcgaccac catcaacgtc
    19261 gccgggccgg ataggtcgat gccttcgtcg aactctgcgc ggtcggggct ggtctcggcg
    19321 aagctgttcc cggtcgagct gaccaccggg gaaacgctgg tgcccgggac tcgctcgggc
    19381 gtcggcgaca gggaacgaac gccggggtag aaggtcagcg gcaggtcgcg ggtgatctga
    19441 tggcgattgt actcggtcac cgcgggcgac gaggcgtcgg tccagtagtg gctggcgccg
    19501 tcgatcacga tcgtgtcgtc caacacgatg ccgtattcgc ccacgaccgg atcgagaccg
    19561 gtcgccacga acggatcgag catgaagtac gcgttgccgc cggcggcgag ataggcgcgg
    19621 atggcctcga cctcgccagg cagcagcgcc gccttcggcc ctgccacgac caggaccgag
    19681 caatcggaca gtgcgtcttc gcccatcacc aacgagatct ttttcacttc gtaattcaag
    19741 gcttcgagac cactgcgcgc cttcgacatc ccgtggcgct cgtagagcac gtattgggtg
    19801 ccgaggccgt ggctgtggcc gggaccggca ccttccgcgt gcgtgtgcga ttccatgctg
    19861 aacggatcag cctcctggtg gccgtcgagg aagcacgcta cctgcgtctt gccctggctg
    19921 acgcgcagga tgccgttggc gatatcggtc tcggtgtcgc cgttgatctg cagcttgcga
    19981 tgctcgcttt ccaggatcgc ggtaccggcg aactggacgc ccagcatcct cgcctgggcc
    20041 gggttgaggg tcggatcgta gaactcgacg gtgaccttgt cagtctgggc ggccatcagc
    20101 tcatacagct cgactgtctc gcgcatcatc gggtcgtgga agaagacgat gtgcaccggc
    20161 tgctccagcc gctcgagcat ggtcgtcgtc tgctcggaca gcgagtacag gcccgcctcg
    20221 gtcatgtcga agcgaagcgg atagcgcgcc gagaagtacc cgagcgcgac caggacgccg
    20281 atcatgccca gcgtgtaaag cgcgatctcg ccgcgccgcc ggcgcatcat cgcgccgagc
    20341 tcgcggcgca acgcaaacgc gccccaggcg gatagcagcg cgcccgccac tgccagcagc
    20401 accgtccaca gcaccgtctc gagcaggatc gcctggatcg cgagagcgct cgcgagcgag
    20461 gcgatcccgg caagcaggat caggccggcg cggaaggaac ggtccacctc accgcctcca
    20521 ttgcagatag ctggcattga gggtcagcgc gatcgcgcac acgatcaggt agaagagcac
    20581 cgcctcgctg cgcaccagtc cttgcacgaa ggcgccgaac tggccggaaa aagacagata
    20641 cccgaacaga gcatacagga catacgatgt ctggaaactc tgcagatggc cgacgaacca
    20701 gaacgcgacc agcaccgaga cggtgatcag ggcggcgacg atctggttct cggtgagcgc
    20761 tgagaagaac tggccgatgg cgaggcacgc catgcccagc agtacgaagc cgatatagcc
    20821 cgcagctatg gtcgtcgttt ccgggtcgcc gaacagataa agcggcacca ggtcgatcag
    20881 ggtcgccgag gtcatcagca gcaggatggt gacggcgccc agatactttc cgagcaccac
    20941 ctgccacggc cgcagcggca aggtttgcag caactccatc gtcccggcgc tgtactcgcc
    21001 gctgaacagg cgcatcgaga ccacgggtat caccagtagc aacaagatgc ccatgttctg
    21061 gaacgtcccg gccatcgagg cgatgccggt gtagaagatg ttgtaggtaa agaagtagcc
    21121 cgtccccagc atgaacacgg cgacgacgaa gtaggcgatc ggcgagcgga aatagtgcca
    21181 gagctccttc tggtacagcg ccagcacgct gctcatgcgg ccttctccac caccggggcc
    21241 gcgtgcacgt agtgaaggaa cacgttctcc agcgtcggct cccgccgctc caggccgtgc
    21301 agctcccacc ggccggccac cacccgtgcg atcgcggcct cgatgccgtc gcgcgcgtcg
    21361 accttgcact cgacggacag ccggcccgca tcgcccgggg ccgggtccat ctgtacggcg
    21421 ttcacgccgt cgaccgacag gagctcctgg cgcagcgcgt ccgcgggggc ggtcgcctgc
    21481 agcgcaacct ggttggtccg ctcgaccgcc tgccgcaggc cctcgggcgt gtcgatcgcg
    21541 agcaagcggc cgtgattgat gatcgccacc cgccggcaga tcatcgtcac ctcgggcagg
    21601 atgtgagtgc tcaacagcac cgcgtgaccc tcgccgaacg aacgtatcgc ctcgcgggtc
    21661 tcatggatct gggccgggtc gagcccgatg gtgggctcgt ccagcagcag cacttggggc
    21721 ttgcccaaca tcgcctgggc gagcccgacg cgctgccggt agcccttcga cagcttgaag
    21781 atctcgcgcc ggaacacgtc ttcgaggtgg caggcggcgg ttacccgctc gagctcggcg
    21841 gccatttccg tacgggacac gcccttgacc ttcgccacga agcgcagata ggcggcgacg
    21901 tcgaggatat cgtagagcgg cgggtgctcg ggcaggtagc cgatctcgcg cgccgcctcc
    21961 acgttctgcg tagccatgtc gaaaccggcc accgtgaccc gccccgaggt ggcggtggtg
    22021 tagccggcga tcatccggat ggtggtcgtc ttgccggcgc cgttgggccc gaggaagccc
    22081 acgatctcgc ccttctccac ggtgaaggac atgtcgtgga tgatggtctt ggcgccgtag
    22141 cgcttggtga cggactcgag cgcgatcatt actttgtgcg cgtaatgcgc cggatcagaa
    22201 acattcggga ggccccgttg cgggggcctc ccgaaaatcg gcgtaaggat gggctattta
    22261 taccacttgc cgcccatctc gctgtactgc ttgatgctgt gtccgaacca cagctttgca
    22321 ttctcgagat cgccgatcat cttcagccga ttgatcgagt tggtgtagtc gaggatgctg
    22381 tagttgaggc cggccggata gccttccatg ttctcttcca tccagaccgc gtcctgggtc
    22441 aggaccaccg tgccggtctc gggcagctgc agcttcaccg actggtggcc gaaggtatgg
    22501 cccggggtgg tcaggatctt gacggtgccg tcgccgaaca ggtcgtagtc gcccttgagc
    22561 gcgaggtagt tgaactcgcg cgcgtcgtca tagtcggcca tgacatgcgc gccgccgcgc
    22621 tggaacttct ccggccacca cgcctgataa agctcgatct gctggatcac gtggatcgcg
    22681 ttcgggaaca tctcgatgtt gccgatgtgg tccaggtgcg agtgcgaggt gatgacgatc
    22741 ttcacgtcgc tggtcttgta gccgagcttt tcgagctgct tgtcgatcac gtcggcccgc
    22801 gtctggccgg gcttcaggaa gtcgcacagc cccttggtcc aatgcgactc gcagttgccg
    22861 tcggagatcg cgacattgtt gccggtgtca tacacgatca ggcccttcgg gtggtcgagg
    22921 acccacatgt tgatcgggat cttgatgtcg cgcgcttccg gatagctcgg gatcagcccc
    22981 tggatgttga acgggccgaa ctgcccgctc gccgtccagt aaagcttctg cgccgatgcc
    23041 gtcgacgcgc cggccatgca cagtccggca agcagcgccg cggcgatacc gagtttcttc
    23101 atgactgtcg tatcctccct acgatgatgg cagcgccccg tcgggcgcgc gtcccgcctt
    23161 cgtcccgccg aattggagct cccgg
//
LOCUS       DASWHU010000042        37451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1122993, whole genome shotgun sequence.
ACCESSION   DASWHU010000042 DASWHU010000000
VERSION     DASWHU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 37451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37451
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122993"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VGB88_01490"
     CDS             <1..174
                     /locus_tag="VGB88_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX9646140.1"
                     /translation="FEVAMAALATLRTPIDAFFDRVTVNTEDARLRENRLRLLSQIRA
                     SLASVADFTRLEG"
     gene            178..2850
                     /gene="ppdK"
                     /locus_tag="VGB88_01495"
     CDS             178..2850
                     /gene="ppdK"
                     /locus_tag="VGB88_01495"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9646141.1"
                     /translation="MVDKWVYRFGDGSAEGHAGMRDLLGGKGANLAEMSRLGLPVPPG
                     FTITTAVCGRFYDNERSYPDGLEAEVDAALSRLEQLTGRRFGDAADPLLLSVRSGAAA
                     SMPGMMDTVLNLGLNDRTVDGLAARANDPRFAFDSYRRFIQMYGDVVLGVAHHHFEDI
                     LESYKDRRGISLDTELCADDWRDVTARYKDVVAKMRGEPFPQDERVQLWGAVGAVFGS
                     WMNQRAVTYRRLHEIPDRWGTAVTVQSMVFGNMGGDCATGVAFTRNPSTGAREVFGEY
                     LINAQGEDVVAGIRTPLPLTRAGRDPGGAGQRSMEETMPEAFAELSGVFEALERHYRD
                     MQDIEFTVQQNRLYMLQTRAGKRTAKAALKIAVDMAEEGLIERAEAIRRVDPASLEQL
                     LHPSLDPDAPRTVLARGLAASPGAATGIMVLSADDAEARAQHGEDVILVRIETSPDDI
                     HGMHAARGILTARGGMTSHAAVVARGMGRPCVAGAGELRIDMRAGTLSAGGVTVRAGE
                     RITIDGSTGEVMLGEVATVKPELGPDFERLMGWVDETRTLKVRANAETPADARTARAF
                     GAEGIGLCRTEHMFFEDERIMAIRQVIMADSEDSRRAALARIEPMQRADFVELFQIMA
                     GLPVTVRLLDPPLHEFLPKGEDEIADMAEATGATIDEVTRRASLLHELNPMLGHRGCR
                     LGLSYPEIYEMQARAIFEAVVEVARDADLSVVPEIMIPLAATGRELELLKERIDRVAG
                     EVAAESGIALDYLVGTMIELPRACLCAGELAATAQFFSFGTNDLTQTAFGISRDDSAA
                     FLNTYQQLRILEADPFVRLDVDGVGELIELAVARGRAARPGLKLGICGEHGGDPASIR
                     FCHRVGLDYVSCSPYRVPVARLAAAQAALESSAT"
     gene            2850..3191
                     /locus_tag="VGB88_01500"
     CDS             2850..3191
                     /locus_tag="VGB88_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646142.1"
                     /translation="MSVRRAVLAVMLAAALHGAPAGAASLEDEMGPELIALADEVVAK
                     VVYGIEYENLKPQQYARVIRAWVDHYGEQGRITKRQGALIQTYVTKMALAHRRGEPYV
                     PYAERTAPGDD"
     gene            complement(3158..3784)
                     /locus_tag="VGB88_01505"
     CDS             complement(3158..3784)
                     /locus_tag="VGB88_01505"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX9646143.1"
                     /translation="MGDAGDGRGRCGWGDGAPDYLAYHDREWGAPLHDERRLFEMLVL
                     EGQQAGLSWLTILRKRDGYRAAFDEFDAGRIAAYDARKQAALLADARIIRNRSKIEAV
                     IANARATLAVRAERGGLDSYLWDFVDGTPLVNRRRRLADIPARTPLSERISKDLRRRG
                     FKFVGPTITYAFMQSIGMVNDHLVGCFRHAELAGRERGQSSPGAVRSA"
     gene            complement(3784..4653)
                     /locus_tag="VGB88_01510"
     CDS             complement(3784..4653)
                     /locus_tag="VGB88_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX9646144.1"
                     /translation="MSERSYAILETRGVVRLAGVDDARGFLQGLVTNDVSAVARGRAV
                     YAGLLSPQGKYLHDFLVFELGDGIALDCERARIGDLIRRLTIYRLRARIDIADVTESY
                     LVAALMDRGTPELAHLDGAAFPDPRLAALGWRAVLPRATAGVRLEAAGYAPAGAHAYE
                     SLRIALGVPDGSRDMVVDKSFLLESNFEELHGVDFAKGCYVGQENTARQKHRGTVRKR
                     LMPVAIDGPPPAPGTPILFAGGDAGTMRSSIDGKGIALLRLEVVDKAAETSQSLVAGD
                     ARLTPLRPGWADA"
     gene            4767..5837
                     /locus_tag="VGB88_01515"
     CDS             4767..5837
                     /locus_tag="VGB88_01515"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9646145.1"
                     /translation="MLAIAQAQSYNAAMLVTFIDDSLPYDGHTPTSQPLGGAEKSVVG
                     LATSLAGRGHVVRVFNGCTFPIVAHGVSWQPLQECRTSRTDWLVVHRKPALLARIADA
                     DRRALLLSTPAGYLLNRDELGAVNRSKPVLVVQGIAHAFTIPVPLQSYSSVTLQLGVG
                     ASYLTSAPMAPNAPPHAVATTHPARGLDWLLTLWCEKIQPQVPAAELHLYSAVLDKGA
                     LGAAVPDKVAPVLAQAQAAAAQGVRILRPAADPEMADAYRKARVHLYPGSARDVLCST
                     LAESQAVGLPAVARPLGAADERLRNGVTGYLAPDDDAFANLAVRMLDDRETFDRLSAQ
                     ARMATRGPDWARAAEELEHALK"
     gene            5834..6796
                     /locus_tag="VGB88_01520"
     CDS             5834..6796
                     /locus_tag="VGB88_01520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9646146.1"
                     /translation="MKLLFITATRIGDAVLSSGVLDALIARHPGARVTVACGPAPAPL
                     FAALPGLDRLLVLEKRPLAAHWLALWLAVAPTHWDLLVDLRDSAVSRLVRARARRILR
                     ARANGVHQVERLADAVGLETPPAPRLWLAPGHRRAAAELLGAGAPVLALGPTANWRGK
                     LWPAERFVELGRRLTAAGGLIPGARIAVLGAPAECVLAQPVVDGLARERVIDLVGRVD
                     LPTAGAVLERCDLFIGNDSGLMHIAAAAGIATLGLFGPSRDEHYAPWGAACAAVRTPE
                     SFDELIGTPGYDHRTTESLMGSLGVEAVEAAAGRLWQRRRAAAS"
     gene            6793..7569
                     /locus_tag="VGB88_01525"
     CDS             6793..7569
                     /locus_tag="VGB88_01525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9646147.1"
                     /translation="MSAAPAPRLSAVVTARNEADQLADCLACLAPADELVVVLDRCTD
                     ASPEIARGFTDRIVEGAWEIEGDRRNAGIDAARGDWLLEVDADERVPAALFDEIRDVI
                     ATAAPGYFLVPFDNYIGDRLVKYGWGASWGVGAAPRLFSRGAKRWGAQRVHPRLELQG
                     PKRRLRTPIAHHVDRNLNDMLDRLKRYTDARAADLRASGAPLPPLRSVARRSLGRFVK
                     CYVQRKGYREGGWGVAIAVMAALYPLLSHLKATLEGDRDR"
     gene            7575..8627
                     /locus_tag="VGB88_01530"
     CDS             7575..8627
                     /locus_tag="VGB88_01530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9646148.1"
                     /translation="MRLLQAIAGARHGGAEIFFARLVAGLARAGVTQRVLSRADEARS
                     AAFRALGIDPVHLAFGGLLDRATGPRFRREIEDFRPDVVLTWMNRATSFCPRVTPAMR
                     FVHVGTPRGYYNAKHYRRCDRLICATEDIAGFYLARGWAAEKVCYIPNFVCPQATAPV
                     AREELETPRGVPLVLALGRLHQNKAFDVLLEAMTRLPRHWLWLAGAGPLERSLKRQAE
                     RLGIADRARFLGWRTDAPALLAAADVLACPSRHEPFGNIIIEAWAQGVPVVAAASEGP
                     GRIVTSGVDGVLVPVEDAHAFARGIESVTGDADLAGRLVAAGRAAHAAAYSEPVVVGK
                     YVAYLANLAEARTCAA"
     gene            8615..10399
                     /gene="asnB"
                     /locus_tag="VGB88_01535"
     CDS             8615..10399
                     /gene="asnB"
                     /locus_tag="VGB88_01535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX9646149.1"
                     /translation="MCGVGGVFTRAVEGPSEAVLDALAEALAHRGPDGRGRYRARGLG
                     LVHTRLAVIDLQTGDQPIIEAGARAGGAAIIANAEIYNYLELREQLDGIAFATASDCE
                     PPLHLYRRHGLDFTHALRGMYALAIHDPAANALVLARDPFGIKPLYYVERAGYFAFAS
                     EPRALLAAGLAERAVDAGARDETLQLQFSTGRDTAFRDIKRVLPGETIVVSDGRIIDR
                     RRLAALPEGGPVAIEEGAALAGLDAMLEETVRHHQRADVPFGLFFSGGIDSTVVLAVM
                     ARLNDRPVRAYTAAFPGTGVADERDQARSLARALGAEHVEVEVTEDAFWRSLPAIAAA
                     VDDACADYAVVPSFELARVAARDLKVVLCGEGGDEILAGYGRYRSVLRPRLLGGRGMR
                     MRGMLDGLDLLRGPPAAWRDGIVAAEATAALPGRSRLQVAQASDCADWLPNDLLVKLD
                     RCLMAHGLEGRTPFLDRPFAEFAFRLPDRLKIRHGRGKWLLRRWLESHLPAAHPFARK
                     RGFTVPVAEWIARQGDRLGPLVAAQPGIAELCRPDAVQALFRRPGKRPGFAAWTLLFH
                     ALWHGIHIQGLETEGDVFEVLAASARGD"
     gene            10502..11947
                     /gene="otsA"
                     /locus_tag="VGB88_01540"
     CDS             10502..11947
                     /gene="otsA"
                     /locus_tag="VGB88_01540"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HEX9646150.1"
                     /translation="MVGSKTQATTKRGSRLVVVSNRVTPVTARKALAGGLAAGVLAAL
                     RDSGGIWFGWSGAVSERPASEPAAHSHDKITYLTTDLSPREFEEYYNGFSNRTLWPLF
                     HYRLDLSVFDRVQYETYLRVNRHLASRLLPYLESDDLIWVHDYHLIPFAEELRKMGVG
                     APIGFFLHTPLPPADLLVALPRHDKLLRSLFAYDLVGFQTDNDVRNFRDYIQREANGR
                     LGEDGLCTAFGRTIRVGAFPIGIDAEDFAALADTPEAVRQRRILEKSVVARDVIIGVD
                     RTDYSKGLPSRLKAFELLLENYPVNRGRVHLLQITPPSREHVPEYGEILAEIEGLSGH
                     INGRFAEFDWVPIRFIHKAYNRRILSGFFRASRVGLVTPLRDGMNMVAKEYVAAQDPD
                     DPGVLVLSRFAGAARQMDRALIVNPYDLSQVADAIQEALEMPLDERRSRWRAMMDDLK
                     RNDIERWRASFVEALQSASAELDRPEETAAE"
     gene            complement(11956..12798)
                     /locus_tag="VGB88_01545"
     CDS             complement(11956..12798)
                     /locus_tag="VGB88_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX9646151.1"
                     /translation="MSLGDVKGYYAALGITPEASAAEVKRAYRARAKEMHPDTGGAGD
                     GTTFRTLTEAYHVLSDAHRRGEYDALCRTLESSARPAAETKAEAPRAGPARPARARET
                     GYAPVRCRRCATVSAQPRYVRLRKVTGRLTRSQVEVIEGAFCRSCADRVALWASLHTW
                     LLGWWSLPRGPLDTLRALATNLAGGDKPAAPNAALLAHQARAFAARGDAALAAGLALQ
                     ANEFRYDAALEPLIAAGGGRRLRDRWRVGGLAFVIQALPVGLGLAWLVLWLGRRALSS
                     TIGG"
     gene            complement(12823..13563)
                     /locus_tag="VGB88_01550"
     CDS             complement(12823..13563)
                     /locus_tag="VGB88_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646152.1"
                     /translation="MFDFSDKVMVMSGANGGITRAIARAFHGCGARMVLSDLDRDGIA
                     AFAAGLDPAGKTVVATGCDVTDSAAVEATVALCEQRFGSADFLITGAGLYLDQLTAGM
                     TDAQWRQTIAINLDGAFHFCRAIQRIIRDGGAIVNIASMAAHRGSFQHAHYSAAKGGV
                     LSLTRSIAIELAPRNVRVNAVSPGLIDTPLIQPLLKVQGDLLMQLTPMKRLGRPEEVA
                     NVILFLCSDLASFVTGETIHINGGLYIS"
     gene            complement(13621..14502)
                     /locus_tag="VGB88_01555"
     CDS             complement(13621..14502)
                     /locus_tag="VGB88_01555"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9646153.1"
                     /translation="MSAAARPPITGKTRVLAIFGRPIGHALSPLIHNAALEAAGLDVV
                     FVPFSPAAEDLERALTGVVAAGCIGLCITIPFKPQVVAMLDELAGSARATGAVNTVVV
                     GAGRRLVGHNTDVDGVLRCIATLPARARGRAVLLGAGGAGRAAAVALVRAGYGELVIA
                     NRTPARGEALAGDLEHGATTVTVAGAERESLAAALAGAALLVNATPVGMYPDNDSTPV
                     PADLLRADLDVIDAIYRPEPTRLCREARAAGAAACTGVDWIIAQGVEALRLWLGFDPD
                     ERAMRAALEAFLGEERG"
     gene            14699..16453
                     /locus_tag="VGB88_01560"
     CDS             14699..16453
                     /locus_tag="VGB88_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646154.1"
                     /translation="MPGSSQQSWSLIYFAIGGGFALAFLVAILALSRARARPYWLFAV
                     LAVPVVALFANPDWRLYSAHALYHYSIVYQIFEHGVPPSLPLAAGVDLKYFYAIHFVI
                     AQLMRLFAFPPAWAFATLGVACLLAMVLVLDRLARRVSDDIPYRWLVLTVILFGSAVL
                     VGDPIGRALANTGVIVREGRLDLLHKFIDINNNQLGLVLYALAFLGLVNLVGEGGRER
                     AGLVQFATGYVLAGVFYPPAWLGVCIAAAVAVGQMVALTRPIPWRKIAQLSAIGVVGL
                     AVLAPWLLSLTVGKSADSAIRPTLGLLARNAPILAAALAVPVLLALYKSELLVRHFRE
                     RPRLFGFLALSMIVLQAAFLLVYLPTDAEYKMINFASVPLGLMFALVLRQMFDQVRVL
                     ATVVLFLVLLPFSQKIGTYLVFGWPVSDPATTAGRYIVDRVPAQQALYQWIAAESPDD
                     AVFIDSYVTVPAFARRPLLIGTEFRRERGELHVAGEMGLRKDGWGPVIDLMLQINGVD
                     PDLAARRLAIAGEFLAETDRRLSDEVFAALRGEVPGRPVYVVARHGAVITRLARDRRL
                     TSVFQNRAATLFRIAAAG"
     gene            16463..18076
                     /locus_tag="VGB88_01565"
     CDS             16463..18076
                     /locus_tag="VGB88_01565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9646155.1"
                     /translation="MKPDTLARFAAPQGIGVGRAARPALALAGVVALAAVARIWGLDR
                     HSLWLDEAFSYHYSGLSLHDIWRAMPLYESHPPVYFTILKAWRAAFGDGEAAMRGLSV
                     AAGTATVALVYLLGRLAGGRRDGHRIGAFAALLFAISPLHLDFAQQARPYALIILATA
                     TTLCGVAWLLRHPAVARSPWLGLAAMRAHPRRAPLLRRGLAAWAATALGVAAALWLHD
                     TVLVYAAALGLFGLWLAWRWGWPRSFVANLMIAGAAALALWLPFLPTFAVQLAGVSRA
                     FWMPDLTWQAALAPVGFLMDVRFVRPLTAAPVLVLLALAGARGVAGRSGAPMAALLIG
                     VAVAGYGLMLLFCLLVQTVLVERTLIWMSVPLLVLVAAGVVGLERRALRAASAVVVLG
                     FSIWGALVFQLAYQREPWRDIAARIGGQAVAGDVVVVMPNSLALPLAYYRDRLGDQVA
                     VHAMPAAFPEISPDRAYPTSVLAEPAFVRADLPALDAAIAGAGSVWVITRLDDIFDPD
                     RLVLGHMRQVRTETEHWPVSGYLHVHRFR"
     gene            18093..19667
                     /locus_tag="VGB88_01570"
     CDS             18093..19667
                     /locus_tag="VGB88_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646156.1"
                     /translation="MAEIVTAGASARRTTVALAALVAVAAALRAWGLERQSLWIDEAY
                     AYWFSSQSLHDLWQVFPAHHNHPPLFMTMMKGWRWLLGDGEAAMRSLSVVPSVLTVPV
                     VFVMGRLAGGAARGAQTGLLAALVFALSPLHIDYAQQAYVYTWFTLGAGLSLAGAFWL
                     LVHARAAERPWLDLRRDTHRAGEARATGAWAATVIGSALALWMHNVAVVYVAALGLSA
                     AWIGWRDGRPRWYFANLVLAGLAVLAVWSPFAAKFAAQVGVVSGNFWVEDVTLASALQ
                     FIPFLFEIRFTWPIKGSVLLVLLAALGLFAISRWGRRDLTRLMLGVAGLAYALLLLYS
                     LLVQSILVDRLLIWVSLPFYVAVAAGLVAMRRRWLRVLLVVAIVALSLQGYVNYQRGF
                     EREPWRNIAAHLAEHAGPDDVVVNLPNDNVIPLAYYLDAGGGSLKLVGLPVPYPSFDE
                     SRLSPVGVIFTPAVEAADLARLDEVTEGAAAVWYVARETRLFDPDQIVLHHLQKTRRL
                     AWHWPVRQHIDVYCFE"
     gene            complement(19652..21013)
                     /locus_tag="VGB88_01575"
     CDS             complement(19652..21013)
                     /locus_tag="VGB88_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX9646157.1"
                     /translation="MARAVFLIGLCALAFIAGAATTTTFEVFPYRQFRDARLALRALL
                     PALGVGQPTVFMGYDKDYSQPTVIAHGNATENDHLILVGGGPYELMSECPEQGCLAWI
                     IDRQGRVVHAWPLDLDLLWGERAREAGAGPLDRLVPMGLHLFANGDLLVSYQSSSAFP
                     YAIGIAKLDKAGNLLWRQQNLSHHWFSLDEGGRIYVPSQRLAPAPVALGGGVEITCET
                     GFVYDDEIKVLDADGRLLRELPVLKALLAADYPGLLHHAEALASEQCDPTHLNDVRVL
                     SPRLASEYPGLAAGDLLVSLRTSNALAVLDGTSGRVKWLLAGRTLAQHSPRFSGANKV
                     LAFDNKGGLDPENLSRLVEIDLATNAVRTVYPVPGGSGAGRFATVFAGHIDLHPDGRR
                     ALVALADQGQIVEIDLASGTVVWEYVNTHPVPGEERPVRMAAMSAYYVPEAGFAAEAH
                     SKQ"
     gene            complement(21039..22145)
                     /locus_tag="VGB88_01580"
     CDS             complement(21039..22145)
                     /locus_tag="VGB88_01580"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9646158.1"
                     /translation="MSETPPNAIEMSASRHRFGALVERAHGLLTRLAALESRWYAARL
                     EAIPIVGPIYVTGLARAGSTILLEVLAAHPEVATHSYRDVPPIQVPLWWNAFLELVPR
                     RAAEPVERAHKDGLLVTPESPEAFEEVLWNQRFADRDAAFDRFYRDHLRKILLVRGGR
                     RYVAKNNYHVARLGYLYELFPDARFVIPVRHPEAHVASLVKQHRLFCREEQRDARILA
                     HMQRLRHFEFGLDRRALELGDRGAADEVRRLWAAGEDAAGYALQWAQVYGFVLDRIGA
                     HPGLAEATMIVRYEDLCDRPETTLRAVYAHCRLEAGAALVEAQARRLHRPDYYRPDFS
                     DHERQAIAAATDAVARRLGYAADAAHAAGDARAV"
     gene            22296..22583
                     /locus_tag="VGB88_01585"
     CDS             22296..22583
                     /locus_tag="VGB88_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9646159.1"
                     /translation="MAAYLIAQIEVTDQEAFDAYRAKVPATIAKYGGRYLVRGGAITT
                     LEESPPKPRVIVLEFESVAAARRWYESPEYRPLIPMRRRAADGPVMIVEGA"
     gene            complement(22671..23135)
                     /locus_tag="VGB88_01590"
     CDS             complement(22671..23135)
                     /locus_tag="VGB88_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX9646160.1"
                     /translation="MRTFDLTPLFRTTVGFDRLNRMLEEASQAFDGGTGYPPYNIEKL
                     DEDTYRITMAVAGFGEDELDVTVTDEMLVVSGRIAGEDAGHTFLHRGIATRAFERRFQ
                     LADHIKVSGAKLENGLLHVELSREIPVEKRPRKIAIGAEAGTHKVIEGGKAA"
     gene            23534..24343
                     /locus_tag="VGB88_01595"
     CDS             23534..24343
                     /locus_tag="VGB88_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9646161.1"
                     /translation="MARPLRAMIRRREPAFGTFVGEFDTPGMGHILAAAGCDFAFLDM
                     EHSGLTLASVKGAVRHYEAAGVALIVRPPSKAYDHIARVLDTGAEGVCLPCVGSAEEA
                     RAIVASAKYPPAGERGIGLQLAHDNYVRGEVAAKLAAANRRSCVVLQIESAEGAENAD
                     AIAAVPGVDALWIGHYDLSASLGIAGQFAHPDFLAAVRRAIDACKRHRRGLGMVVGSP
                     EQGLDMIGAGFNLIAYSGDVWLLRDALSAGIDAMRDGARVRLGAETGRGKA"
     gene            complement(24473..24694)
                     /locus_tag="VGB88_01600"
     CDS             complement(24473..24694)
                     /locus_tag="VGB88_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646162.1"
                     /translation="MITLSVALLCACAGDGAKPHYCEQGILSTAANQIFNPELFGVML
                     VVRTAMEPWCIDAPDRGRAPDGSGRRIDR"
     gene            complement(24791..25129)
                     /locus_tag="VGB88_01605"
     CDS             complement(24791..25129)
                     /locus_tag="VGB88_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646163.1"
                     /translation="MIQLFTTTRFGLSILASALMVSGCASTEPNTASIAADSPQQAES
                     CGRVEAAAEFSMGFFATMDDMAEACANGDVFACNPANYPLMAIAGVFFAPMGFLIGLA
                     SQDVEDHVCG"
     gene            complement(25296..25628)
                     /locus_tag="VGB88_01610"
     CDS             complement(25296..25628)
                     /locus_tag="VGB88_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646164.1"
                     /translation="MTRLSCGIAVVLTAYAVSGCAFARPAGDERFSYSPPEDISEVHK
                     SECQARARRAAFDRVAEMSDEAETIAILTGGLGAWLTLEYAASAEDRAYEETFKDCLR
                     EKGHDISE"
     gene            complement(26313..27005)
                     /locus_tag="VGB88_01615"
     CDS             complement(26313..27005)
                     /locus_tag="VGB88_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646165.1"
                     /translation="MSRQTNVLAVALVILAAPFLHACATVVKGSTQPFTVATDPGRAK
                     CEISRDGAMVGAVSSTPGTIQISKSANGLEIACTRDGYLDTRQTVASGTSGWAAGNIL
                     FGLLGGGVGLVVDAASGSMSEYPETVRFALYPASFPTAEARDRFFATRAEDIEAKFRL
                     VEEQFDRSCAQASAGRQTRNDCEDNKAALKAASDRSLAELEQQKQSAVIADDQIAAEL
                     RTSDRPYGGAQP"
     gene            complement(27002..28567)
                     /locus_tag="VGB88_01620"
     CDS             complement(27002..28567)
                     /locus_tag="VGB88_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9646166.1"
                     /translation="MGKRVLVVNAFFDEYRRTAGSPHRVPRAMGHVFLAGAFERSTTE
                     VRLYNEQYSGPLHDAALLGSPDLLVLTGVTSSFDRMLHLAAYARTLNPRVIVAAGGPA
                     IRALPRRAARFFDYPCTGDIEALQEVARDALGPAHVAEEMFPRFDLADPHRLVAYVES
                     SRNCNFRCGFCSLTGEGGRYRHYEIDYLKRQIEAVGKKQIVLIDNNFYGNDRRFFLDR
                     VALLRDFYRSGRIDGWSALVTGDFFARRENLDLVREAGCQSLFSGVESLDAETLRAHN
                     KRHSTVVPQLEMIRTCLEAGVLFSYGIMLDPSVRRLEKLRAEIGLLLDTPTITLPAYF
                     TLSIPLLGTPYFYDCLAQHRLLPHVRLRDLDGVTLALRSLDRLEEAVGFARDLPSLRG
                     YRTRVLRHAVRFLRHYRRRLTPLQLFAAMVTSALISNETFASSPLRLRLRRPRRTYLA
                     STEELDPLYRPFMRLPARFEAYFRPTMVSDAHGALHDDVADDVDPDARSERRHFGIPS
                     VACGPSDNRRPTS"
     gene            28917..30971
                     /locus_tag="VGB88_01625"
     CDS             28917..30971
                     /locus_tag="VGB88_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX9646167.1"
                     /translation="MAQAFLGYLAAHSELAEGRETLAGLFWGDRFRDQARHSLRQTVL
                     VLRKAFEDAGAQALAADAHSVALDPAVVTVDVQRFRALAASEQRADLDEACALYRGEL
                     LADVSVRADGFTTWLDREREQLRQLAADAFMRLARLEAAEGAHDAAIAAATRALAIEP
                     ALEDCHRELMRLYRLQGNRSAALRQYQACVEALRRELDVDPDPATVRLADEIRAARDT
                     PPPTATGGSTQPAQPSQWRTWLPSRRWRRLSPYLAGGLLLLLGAIGGVALLTGVERVR
                     EEARQERMLFPLPAQPSIVVLPFKNLNRDPAYQVMVDGMTEEITAALSMVSEMFVIAR
                     TTALTYRDRAVDVREVAEELGIRYVLDGAVQVEEDRLRVLVELVDAIENRQVWTHRYE
                     REMTSLFEINDDITLEVVTGLQVQITEGEQDRISLVHGTDNLQAWLVAGRALQHLRRL
                     TREDNARARGLYRQAIALDPSYPGAYDGLSWTYFIDARFGWTERPDEAIRQAGELAQR
                     TLELDPERPATYALLGGVQLMLGNHDQAVALAEKAVELSPSGADVTAILALILTYVGD
                     YRRSVALFQRAMRLSPSYPDWYRWSLGRAYRLLGRHRDAEATLAFEATEAGGSIAPLV
                     ELAATYAEFGKMREARQVARRVAAVAPGFSATAWTRMPPHRDQATAAAERAALIRAGL
                     PE"
     gene            31140..32180
                     /locus_tag="VGB88_01630"
     CDS             31140..32180
                     /locus_tag="VGB88_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646168.1"
                     /translation="MSKFRVAISGDLLKDDGTPAIPEFDLSRLEREADVEYAYVGRGP
                     VLDAGELEDFDALILMMPRFAPASVPKSGRLAVVARGGVGYDTVDVAACTEAGIALVI
                     TPDAVRRPVAVMVITFMLALSGHLFALDRITRKGPWRARTDYMGVGLIGRTFGQIGIG
                     NIGAETLRLARPFGLELIAHDPYVDPSIAKDLGVRLVDMETVFRDSDFLSVSCPLNDE
                     TRGLVNARRLALMKPTAFLINTSRGPVVDQRALTDALRRRAIAGAGLDVLEKEPPDPA
                     DPILTLDNVIVTPHALCWTDQMFATMAEKDIDACLAVMRGRAPQNVVNKAVLDSPRFT
                     DKLTAYRNAFGK"
     gene            32368..34503
                     /locus_tag="VGB88_01635"
     CDS             32368..34503
                     /locus_tag="VGB88_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9646169.1"
                     /translation="MSGLRDLTPLLKPRSVAIIGATPDARRPGGRPLDYLRRFGFAGP
                     IYPVNPRYREIAGVRCYASVADIAEAPDMAVVSVPAAATLDAVRDCQRAGVPAMTIYT
                     SGFGETGAEGKALEAELIALAEAKGTLICGPNCQGVANFHDRMVANFTSALANEGIDA
                     GGIGFVSQSGLFAGIVTAEFRRRGLGLGYLVSSGNEAVVDFADVLAFMARDERIRVVA
                     GYLEGVRDGRKLAEAAAVARELAKPVVLIKVGRSQDSAAAAASHTGALAGAYAVYAAA
                     LAQWGILEADSIPELFDLIETFAVGARPARGDRVGVITNSGGIGVYCADKVRECGLTM
                     ASFASGTVEALKARLPAFGSARNPVDFTLQAYTDPESVGAHLRNVVLDDGVDVALAFF
                     GIQLLNVDAVVDQVAAANALNDKPVLVGWMLGDASGPPRLRAAGIPCFDDPLAAIKAA
                     KALVRQGRIAATAAATRTPHAMDAAAALVAGHAPGALSESEARAVLAAAGIAPVPGAR
                     AADAEAAVAVAREIGYPVVLKVDSPDIAHKTEAGGVALDVRDDDGVRAAFAAITAAAR
                     AHVPKARIRGVGVYRMVRGGVEMICGLSRDPAFGAVVLVGSGGILVEVIEDTALGVAP
                     LEEADAERMIASLKGAALLAGVRGRPPADTRALTEVLLRLSDLARACPAIAELDINPL
                     LVLPEGKGALAADALITLGGEAGRKADSK"
     gene            complement(34685..35074)
                     /locus_tag="VGB88_01640"
     CDS             complement(34685..35074)
                     /locus_tag="VGB88_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9646170.1"
                     /translation="MRPARAGGARGGRRAGSRAGPRRCGPGSRRCRKRRRNEAGLARP
                     MSKEPATLTERRARMAGEDGKAVNRRRRRIETVNADFKNHGLHRFRPRGLDKVRCAAL
                     LHAIAHDIRRGIALGWRRWLPRPLVAA"
     gene            35120..35206
                     /locus_tag="VGB88_01645"
     tRNA            35120..35206
                     /locus_tag="VGB88_01645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35154..35156,aa:Leu,seq:taa)
     gene            35496..36146
                     /locus_tag="VGB88_01650"
     CDS             35496..36146
                     /locus_tag="VGB88_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX9646171.1"
                     /translation="MAAAVEIVAGFRALATSTIGNVLDELGVGGLIVNLRPLVAKRPF
                     CGPAVTAQETHGPFGSYAPEDFRVGAILDAAGAGDVLVIANDGAQVSTWGGLASLAAK
                     RKGIAGAVIDGGARDADEIEACGFSVYARHLVATGGRKRIRVDAIGVPVVVDGVAVSP
                     GDIIVADSTGIACVPAAAAEQVLRLARQFDANDRASAKLLDQGLSLADAMRRYDTI"
     gene            complement(36255..36692)
                     /locus_tag="VGB88_01655"
     CDS             complement(36255..36692)
                     /locus_tag="VGB88_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9646172.1"
                     /translation="MMSPLHRAFAAAAALILIPTASASAQDVRMNLEPSAGEFRNSCA
                     SCHGVDGKGAGFLMRLFKGMDPGDLTQLAKNNGGVFPFDRVLQVIDGRAEVAAHGDRD
                     MPVWGDRYMRQYVSEYGPDESNEFWVRARVLELVYYIQSIQQP"
     gene            36871..37002
                     /locus_tag="VGB88_01660"
     CDS             36871..37002
                     /locus_tag="VGB88_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646173.1"
                     /translation="MPASAAEDVLNLARRFDANDRTSAKPLDEKLSLAEPMRRFDAI"
     gene            complement(36999..>37451)
                     /locus_tag="VGB88_01665"
     CDS             complement(36999..>37451)
                     /locus_tag="VGB88_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9646174.1"
                     /translation="GVAAGAGYQLALLCDLRIGHAGVRMGQPEINAGLASVIGVHLMS
                     LTLGHAQTRDLALTGRLVEAEEALRLGLLTRLVPAAEVLDEARAAVRELAAKPPNALR
                     LTKRRLRQMTQAGFDEVFEAAVPLQRAAYESGEPQAAMAKFFARTKRG"
BASE COUNT         5227 a        13164 c        13273 g         5787 t
ORIGIN      
        1 ttcgaggtcg cgatggcggc gctcgcaacc ctgcgcacgc cgatcgacgc gttcttcgac
       61 cgggtcacgg tcaacaccga ggacgcgcgc ctgcgcgaga accggctgag gctcttgtcg
      121 cagattcgcg cctcgctggc gagcgtcgcc gacttcaccc gcctcgaggg ctgactcgtg
      181 gtcgacaagt gggtctaccg gttcggcgac ggcagcgcgg aggggcacgc gggaatgcgc
      241 gacctcctcg gcggcaaggg cgccaacctc gccgagatga gccgtctcgg cctgcccgtg
      301 ccgcccgggt tcaccatcac caccgcggtc tgcggccgct tctacgacaa cgagcgcagc
      361 tatccggacg ggctcgaggc cgaggtcgac gcggcgctgt cgcggctcga gcagctgacc
      421 gggcggcggt tcggcgatgc cgccgatccg ctgctactgt cggtgcgctc gggcgcggcg
      481 gcgtcgatgc cgggcatgat ggacacggtg ctcaacctcg gcctcaacga ccgcaccgtc
      541 gacggccttg ccgcgcgcgc caacgacccg cgcttcgcct tcgacagcta ccgccgcttc
      601 atccagatgt acggcgacgt ggtgctcggc gtcgcccatc atcacttcga ggacatcctc
      661 gaaagctaca aggatcggcg cggcatttcg ctcgataccg agctttgcgc cgacgactgg
      721 cgcgacgtca ccgcccgtta caaggacgtg gtcgcgaaga tgcggggcga gccgttcccg
      781 caggatgagc gcgtccagct gtggggcgcg gtcggcgcgg tgttcggaag ctggatgaac
      841 cagcgcgcgg tcacctatcg gcggctgcac gagattcccg accgctgggg caccgccgtc
      901 accgtccaga gcatggtgtt cggcaacatg ggcggggact gcgccaccgg ggtcgcgttc
      961 acccgcaacc cctcgaccgg cgcgcgcgag gtgttcggcg agtacctgat caacgcccag
     1021 ggcgaggacg tggtcgccgg catccgcacg ccgttgccgc tgacccgcgc cggccgcgat
     1081 cccggcggcg ccggccagcg gtcgatggaa gagaccatgc ccgaggcctt cgccgagctg
     1141 tcgggcgtgt tcgaggcgct cgagcgccac taccgcgaca tgcaggacat cgagttcacg
     1201 gtccagcaga accggctcta catgctgcag acgcgcgccg gcaagcgcac cgccaaggcg
     1261 gcgctgaaga tcgcggtcga catggccgag gaagggctga tcgagcgcgc cgaggcgatc
     1321 cggcgcgtcg atccggcctc gctcgagcag cttctccatc cgagcctcga ccccgacgcg
     1381 ccgcgtaccg tgctcgcgcg cgggcttgcc gcctcgcccg gcgccgcgac cgggatcatg
     1441 gtgctcagcg ccgacgacgc ggaggcgcgc gcgcagcacg gcgaggacgt gatcctggtc
     1501 cgcatcgaga ccagccccga cgacatccac ggcatgcacg cggcgcgcgg catcctgacc
     1561 gcgcgcggcg gcatgaccag ccacgcggcg gtggtcgcgc gcggcatggg ccggccgtgc
     1621 gtggcgggcg cgggcgagct gcgcatcgac atgcgcgccg gcaccctcag cgccggcggc
     1681 gtcaccgtgc gcgccggcga gcggatcacc atcgacggct ccaccggcga ggtcatgctc
     1741 ggcgaggtgg cgacggtgaa accggaactc ggccccgatt tcgagcggct gatgggctgg
     1801 gtcgacgaga cgcgcacgct caaggtgcgc gccaacgccg agacgccggc ggacgcccgc
     1861 accgcgcgcg ccttcggcgc cgagggcatc ggcctgtgcc gcaccgagca catgttcttc
     1921 gaggacgagc ggatcatggc catccgccag gtgatcatgg ccgacagcga ggacagccgg
     1981 cgcgcggcgc tcgcccgcat cgagccgatg cagcgcgccg acttcgtcga gctgttccag
     2041 atcatggccg gcctgccggt gaccgtgcgc ctgctcgatc cgccgctgca cgagttcctg
     2101 cccaagggcg aggacgagat cgccgacatg gccgaggcca ccggcgccac catcgacgag
     2161 gtcacgcgcc gggcgagcct gctgcacgag ctcaacccga tgctcggcca tcgcggctgc
     2221 cggctcgggc tcagctaccc tgagatctac gagatgcaag cgcgggcgat cttcgaggcg
     2281 gtggtcgagg tcgcgcgcga tgcggacctt tccgtggtgc ccgagatcat gatcccgctc
     2341 gccgccacgg ggcgcgagct cgagctgctc aaggaacgca tcgaccgcgt cgccggcgag
     2401 gtcgcggccg agtccgggat cgcgctcgac tatctggtcg gcaccatgat cgagctgccg
     2461 cgggcctgcc tctgcgccgg cgagctcgcc gcgacggcgc agttcttcag cttcggcacc
     2521 aacgacctca cccagaccgc gttcggcatc agccgtgacg attcggcggc gttcctcaac
     2581 acctatcagc agctccgcat cctggaggcc gatccgttcg tcaggctcga cgtcgacggg
     2641 gtcggcgagc tgatcgagct tgccgtcgcg cgcgggcgcg cggcgcgccc cggcctcaag
     2701 ctcggcattt gcggcgagca tggcggcgat ccggcctcga tccggttctg ccaccgcgtc
     2761 ggcctcgact acgtgtcgtg ctcgccctac cgggtgccgg tggcgcgcct cgccgcggcg
     2821 caggccgcgc tcgagtcgtc cgccacctga tgtcggtccg gcgcgccgtc ctcgccgtca
     2881 tgctcgcagc ggcgctgcac ggcgccccgg cgggcgcggc gagcctcgag gacgagatgg
     2941 ggccggagct gatcgcgctc gccgatgagg tcgtggcgaa ggttgtctac ggcatcgagt
     3001 acgagaacct caagccgcag cagtacgcgc gcgtgatccg cgcctgggtc gaccactacg
     3061 gcgagcaggg gcggatcacc aagcgccagg gtgcgctgat ccagacctac gtcaccaaga
     3121 tggcgctcgc ccaccgccgc ggcgagcctt acgttcccta cgccgagcgc acggctccgg
     3181 gtgacgactg accacgctcg cgcccggcga gctcggcgtg gcggaagcag ccgaccaggt
     3241 ggtcgttgac catgccgatc gactgcatga acgcgtacgt aatggtcggg ccgacgaact
     3301 tgaagccgcg ccggcgcagg tccttggaga tgcgctccga cagcggcgtg cgcgccggga
     3361 tgtcggcgag ccggcgccgg cggttgacca acggcgtgcc gtcgacgaag tcccacagat
     3421 acgagtcgag gccgccgcgc tcggcgcgca ccgccagggt cgcgcgcgcg ttggcgatca
     3481 cggcctcgat cttgctgcgg ttgcggatga tgcgcgcgtc ggccagcagc gccgcctgct
     3541 tgcgcgcgtc gtaagccgcg atgcgcccgg catcgaactc gtcgaacgcg gcgcgatagc
     3601 cgtcgcgctt gcgcaggatc gtgagccagc tcagcccggc ctgctggcct tcgagcacca
     3661 gcatctcgaa cagccgccgc tcgtcgtgca ggggtgcgcc ccattcgcgg tcgtgatagg
     3721 cgagatagtc gggcgcgccg tcaccccagc cgcagcggcc gcgcccgtcg ccggcgtcgc
     3781 ccatcaggcg tcggcccagc cgggcctgag cggggtgagg cgggcatcgc ccgcgaccag
     3841 cgactggctg gtttcggcgg ccttgtcgac gacctcgagg cgcagcagcg cgatgccctt
     3901 gccgtcgatc gacgagcgca tggtgccggc gtcgccgccg gcgaacagga tcggcgttcc
     3961 cggcgccggc ggcgggccgt cgatggcgac cggcatcagg cgcttgcgca ccgtgccgcg
     4021 gtgcttctgg cgggcggtgt tctcctggcc gacgtagcag cccttggcga aatcgacgcc
     4081 gtgcagctcc tcgaagttgc tttccagcag gaacgacttg tcgaccacca tgtcgcggct
     4141 accgtcgggc acgccgagcg cgatcctgag cgattcgtag gcgtgtgcgc cggccggcgc
     4201 gtagcccgcg gcctcgaggc gcacgccggc ggtcgcgcgc ggcagcaccg cgcgccagcc
     4261 gagagcggcg aggcgagggt ccgggaaggc ggcgccgtcc aggtgcgcga gctcgggcgt
     4321 gccgcggtcc atcaaggcgg ccaccaggta gctctcggtg acgtcggcga tgtcgatcct
     4381 ggcgcgcagg cggtagatcg tgagccggcg gatcaggtcg ccgatgcgcg cgcgctcgca
     4441 gtcgagcgcg atgccgtcgc cgagctcgaa taccaggaag tcgtgcagat acttgccctg
     4501 cgggctcagc aggccggcgt agacggcgcg gccgcgcgcc accgcggaca cgtcgttcgt
     4561 gaccaggccc tggaggaagc cgcgggcgtc gtccaccccc gccaggcgca ccacgccgcg
     4621 cgtttccagg atcgcatagc ttcgctcgct catgcccgct gccgccgtcc gcgcgccgcg
     4681 ctccgccgtg acctcggccg gccggccgcc cggtctagat aggccctaga taggccgcag
     4741 agccggcgcg cgcaagcgcg tggcggttgc tggcaatcgc ccaggcgcag tcgtataacg
     4801 ccgccatgct ggtcacgttc atcgacgact cgcttcccta tgacggccat acccctacca
     4861 gccaaccgct cggcggcgcc gagaagtcgg tggtcgggct cgccacctcg ctcgccgggc
     4921 gcggccacgt ggtgcgggta ttcaatggct gcacattccc gatcgtcgcc cacggcgtca
     4981 gctggcagcc gctccaggag tgccggacgt cgcggaccga ctggctggtc gtccatcgca
     5041 agcccgcgct gctcgcccgc attgcagacg cggaccggcg cgcgctgctg ttgtcgacgc
     5101 ccgccggcta cctgctcaac cgcgacgagc tcggcgcggt caaccgctcg aagccggtgc
     5161 tggtggtgca gggcatcgcc catgccttca ccatcccggt gccgttgcag agctacagct
     5221 cggtgacctt gcagctcggc gtcggcgcct cgtacctgac cagcgcgccg atggcgccga
     5281 acgcgccgcc gcacgccgtc gccaccaccc atccggcgcg cggcctcgac tggctgctca
     5341 cgctgtggtg cgagaagatc cagccgcagg tgccggcggc cgagctgcac ctctactcgg
     5401 cggtgctcga caagggtgcg ctcggcgcgg cggtgcccga caaggtcgca ccggtgctcg
     5461 cccaggcgca ggccgcggcg gcgcaaggcg tgcgcatcct gcggccggcg gcggacccgg
     5521 agatggccga tgcctatcgc aaggcgcgcg tccacctcta tccgggcagc gcgcgcgacg
     5581 tgctgtgcag cacgctcgcc gagtcgcagg cggtcgggct gccggcggtg gcgcggccgc
     5641 tgggcgcggc cgacgagcgc ctccgcaatg gcgtcaccgg ctacctggcg cccgacgatg
     5701 atgcgttcgc caaccttgcg gtgcgcatgc tcgatgaccg cgagaccttc gaccggctga
     5761 gcgcgcaggc gcggatggcc acgcgcgggc cggactgggc gcgcgccgcc gaggagctgg
     5821 agcacgcgct caagtgaagc tgttgttcat caccgccacc cgcatcggcg acgcggtgct
     5881 gtcgtcgggc gtgctcgacg cgctgatcgc gcgccatccg ggcgcgcgcg tgacggtggc
     5941 ttgcggcccg gcgccggcgc cgttgttcgc cgcgctgccc gggctcgatc gcctgctggt
     6001 gctggagaag cggccgctcg cggcgcactg gcttgcgctg tggctggccg ttgcgccgac
     6061 gcattgggat ttgctggtcg acctgcgcga ctcggcggtc tcgcggctgg tgcgtgcgcg
     6121 cgcgcgccgg atccttcggg cgcgcgcgaa cggcgtccac caggtcgagc ggctggcgga
     6181 tgcggtcggc ctcgagacgc cgccggcgcc gcggctgtgg ctcgcgcccg ggcaccggcg
     6241 cgccgcggca gagctgctcg gcgccggcgc gcccgtgctc gctctcgggc cgaccgccaa
     6301 ctggcgcggc aagctgtggc cggcggagcg gttcgtcgag ctcggccgcc ggctgaccgc
     6361 ggccggcggc ctcattcccg gcgcgcgcat cgcggtgctc ggcgcgccgg cagagtgcgt
     6421 gctcgcccag ccggtggtcg acggcctggc gcgcgagcgc gtcatcgacc tggtcggccg
     6481 cgtcgacctg ccgaccgcgg gcgccgtgct cgagcgctgc gatcttttca tcggcaacga
     6541 ttccggcctg atgcacatcg ccgccgcggc ggggattgcg acgctcggcc tgttcgggcc
     6601 gagccgcgac gagcactacg cgccgtgggg agcggcgtgc gcggcggtgc gcacgcccga
     6661 gagcttcgac gagctgatag gcacgcccgg ctacgaccac cgcaccaccg aaagcctgat
     6721 gggctcgctc ggcgtcgagg cggtggaggc tgccgcgggg cggctgtggc agcgccggcg
     6781 cgcggcggcg tcgtgagcgc cgcaccggcc ccgcgtttgt cggcggtggt caccgcgcgc
     6841 aacgaggcgg accagctcgc cgactgcctc gcctgtctgg cgccggccga cgagctggtg
     6901 gtggtgctcg accgctgcac cgatgcctcg cccgagatcg cccgcgggtt caccgaccgg
     6961 atcgtcgaag gcgcgtggga gatcgagggc gatcggcgca acgccggcat cgacgccgcg
     7021 cgcggcgact ggctgctcga ggtcgatgcc gacgagcgcg tgccggcggc cctgttcgac
     7081 gagatccggg acgtgatcgc gaccgcggct ccgggctact tcctggtccc gttcgacaac
     7141 tatatcggcg accgcctggt caagtacggc tggggcgcat cgtggggagt gggtgcggcg
     7201 ccccgcctgt tctcgcgcgg cgccaagcgc tggggcgcgc agcgtgtgca tccccgactc
     7261 gagcttcagg ggccgaagcg gcggctccga accccgatcg cccatcacgt cgaccgcaac
     7321 ctcaacgaca tgctcgaccg cctgaagcgc tacaccgacg cccgcgccgc tgatttgcgc
     7381 gcatccggcg cgccgctgcc gccgctgcgc tcggtcgccc ggcgcagcct cgggcggttc
     7441 gtcaagtgct acgtccagcg caaaggctat cgcgagggcg gctggggggt tgcgatcgcg
     7501 gtgatggcgg cgttgtatcc gctgctctcg cacctcaagg cgacgctcga aggcgaccgg
     7561 gaccgatagc gaccatgcgt ctgttgcagg ccatcgccgg cgcgcgccac ggcggcgcgg
     7621 agatcttctt cgcccggctg gtcgccggcc tcgcccgcgc cggtgtgact cagcgcgtgc
     7681 tcagccgcgc cgacgaggcg cgctcggcgg catttcgcgc gcttggcatc gatccggttc
     7741 acctcgcctt cggcggcctg ctcgaccgtg ccaccggtcc ccggttcagg cgcgagatcg
     7801 aggacttccg gcccgacgtg gtgctgacct ggatgaaccg tgcgacctcg ttctgcccgc
     7861 gcgtgacgcc cgcgatgcgg ttcgtccatg tcggcacccc gcgcggttac tacaatgcca
     7921 agcattatcg ccgctgcgac cggctgatct gtgccaccga ggacatcgcc ggcttctacc
     7981 tcgcgcgcgg ctgggcggcg gagaaggtgt gctacatccc caacttcgtg tgcccgcagg
     8041 cgacggcgcc ggtcgcgcgc gaggagctgg agacgccccg gggcgtgccg ctggtcctcg
     8101 ccctcggccg gctgcaccag aacaaggcct tcgacgtgct gctcgaggcg atgacccgtc
     8161 tgccgcgcca ctggctttgg ctcgccggcg cgggaccgct ggaaaggtcg ctgaagcgcc
     8221 aggcggagcg gctcggcatc gccgatcggg cgaggttcct cggctggcgc accgatgccc
     8281 cggcgctgct cgccgcggcc gacgtgctcg cctgcccgtc gcgccacgag ccgttcggca
     8341 acatcatcat cgaggcctgg gcccagggcg tgccggtggt ggcggcggcg agcgaaggtc
     8401 cggggcggat cgtgacctcg ggcgtcgacg gcgtgctggt gccggtggag gacgcgcatg
     8461 cattcgcccg cggcatcgag tcggtcaccg gcgacgcgga cctcgccggc cggctggtcg
     8521 cggccgggcg cgccgcgcac gccgcggcgt acagcgagcc ggtcgtggtc ggcaagtacg
     8581 ttgcgtatct cgccaacctc gccgaggcgc ggacatgtgc ggcgtagggg gcgtgttcac
     8641 acgcgccgtc gagggcccgt cggaggcggt cctcgatgcg ctcgccgaag cgcttgccca
     8701 tcgcgggccc gacggccgcg gccgctatcg cgcgcgcggc ctcggcctgg tgcacacgcg
     8761 gcttgcggtc atcgaccttc agaccggcga ccaaccgatc atcgaggcgg gcgcgcgcgc
     8821 cggcggcgcg gcgatcatcg ccaatgccga gatctacaac tatctggagc tgcgcgagca
     8881 actggacggc atcgcgttcg cgaccgcctc ggactgcgag ccgccgctgc acctgtaccg
     8941 acgccacggc ctcgacttca cccacgcctt gcgcggcatg tacgcgctcg ccatccacga
     9001 ccccgccgcc aacgcgctgg tgctggcgcg cgatccgttc ggcatcaagc cgctctatta
     9061 tgtcgagcgt gcgggctatt tcgccttcgc ctccgagccg cgggcgctgc tcgccgccgg
     9121 gctcgccgag cgcgccgtcg atgcaggggc gcgcgacgag accttgcagc tccagttctc
     9181 gaccgggcgc gacacggcct tccgcgacat caagcgggtg ctgccgggcg agacgatcgt
     9241 ggtcagcgac ggccggatca tcgaccgccg ccggctggcg gcgctgcccg agggcgggcc
     9301 ggtggcgatc gaggaggggg cggcgctcgc cggtctcgac gccatgctcg aggagaccgt
     9361 ccgccaccac cagcgcgcgg acgtgccctt cggcctgttc ttctcgggcg gcatcgactc
     9421 gaccgtggtg ctggcggtga tggcgcggct caacgaccgg ccggtgcgcg cctacacggc
     9481 ggcctttccg ggcaccggcg tcgccgacga gcgcgaccag gcgcgcagcc tcgcgcgcgc
     9541 tctcggcgcc gagcatgtcg aggtcgaggt caccgaggac gcgttctggc gaagcctgcc
     9601 cgcaatcgcg gccgcggtcg acgatgcctg cgccgactac gcggtggtgc cgagcttcga
     9661 gcttgcccgc gtcgccgcgc gcgacctcaa ggtggtgctg tgcggcgagg gcggcgacga
     9721 gattctcgcc ggctatggcc gttaccgcag cgtgcttcgc ccgcgcctcc tgggcgggcg
     9781 cggcatgcgc atgcgcggca tgctcgacgg gctcgacctg ttgcgaggcc cgcccgcggc
     9841 ctggcgcgac ggcatcgtcg ccgccgaggc gaccgcggcg ctgccgggac gcagccggct
     9901 gcaggttgcc caggccagcg attgcgccga ctggctgccc aacgacctgc tggtcaagct
     9961 cgaccgctgc ctgatggcgc acgggctcga aggccgcacg ccgttcctcg accggccgtt
    10021 cgccgagttc gcgttccggc tgccggaccg gctgaagatc aggcacggcc gcggcaagtg
    10081 gctgctccgg cgctggctcg aatcccacct gccggcggcg cacccgttcg cgcgcaagcg
    10141 cggcttcacc gtgccggtgg ccgagtggat cgcgcgccag ggcgaccggc tcggcccgct
    10201 ggtcgccgcc cagccgggca tcgccgagct gtgccggccc gacgcggtgc aggcgctgtt
    10261 ccgccgcccc ggcaagcgcc cgggcttcgc cgcctggacg ctgctgttcc acgccttgtg
    10321 gcacggcatc cacatccaag gccttgaaac ggaaggcgat gtgttcgagg tgctggccgc
    10381 ttccgcacgc ggcgactaag cgtttcgatc gccggggcga cagcggcggc gaaagggttt
    10441 atgctcgccg gaccggccgg gagggtattg gcgcccgccg gccggccgcg gcggggaaag
    10501 catggtcggt agcaagacgc aggcgacgac gaagcgcggt tcgcgcctgg tcgtggtctc
    10561 caaccgcgtc accccggtga ccgcgcgcaa ggcgttggcc ggcggcctcg ccgcgggcgt
    10621 gctcgccgcg ctgcgcgaca gcggcggcat ctggttcggc tggagcggcg cggtcagcga
    10681 gcggcccgcc tcggagccgg ccgcccacag ccacgacaag atcacctacc tgaccaccga
    10741 cctgtcgccg cgcgagttcg aggagtacta caacggcttc tccaaccgca ccttgtggcc
    10801 gctgttccac taccggctgg acctgagcgt cttcgaccgc gtccagtacg agacctatct
    10861 gagggtcaac cgccatctcg ctagccgcct gctgccctat ctcgagtccg acgacctgat
    10921 ctgggtgcac gactaccacc tgatcccgtt cgccgaggag ctgcgcaaga tgggggtggg
    10981 cgcgccgatc gggttcttcc tgcacacgcc gctgccgccg gccgacctgc tggtcgcgct
    11041 gccgcgccac gacaagctgc tgcgctcgct gttcgcctac gacctggtcg gcttccagac
    11101 cgacaacgac gtgcgcaact tccgcgacta catccagcgc gaggccaacg gacggctcgg
    11161 cgaggacggc ctgtgcacgg ccttcggccg caccatccgc gtcggcgcgt tcccgatcgg
    11221 catcgacgcc gaggatttcg cggccctcgc cgacacgccc gaggcggtgc gccagcgccg
    11281 catcctcgag aaaagcgtcg tcgcccgcga cgtcatcatc ggcgtcgacc gcaccgacta
    11341 ctccaagggc ctgccgagcc ggctgaaggc gttcgagctc ctgctcgaga actatccggt
    11401 caatcgcggg cgcgtccatc tgctccagat cacgccgccg tcgcgcgagc acgtgcccga
    11461 gtacggcgag atcctggccg agatcgaggg cctgtcgggt cacatcaacg gccgcttcgc
    11521 ggagttcgac tgggtgccga tccgcttcat ccacaaggcg tacaaccggc gcatcctgtc
    11581 cggcttcttc cgcgcctcgc gggtcgggct ggtgacgccg ttgcgcgacg gcatgaacat
    11641 ggtcgccaag gagtacgtcg ccgcccagga cccggacgac ccgggcgtgc tggtgctgtc
    11701 gcgtttcgcc ggcgccgcgc gccagatgga ccgcgccctg atcgtcaatc cctatgacct
    11761 cagtcaggtc gcggatgcga tccaggaagc gctcgagatg ccgctcgacg agcgccggtc
    11821 gcggtggcgg gcgatgatgg acgacctcaa gcgcaacgac atcgagcgct ggcgcgcgag
    11881 cttcgtcgag gcgctgcagt cggcatcggc cgagctcgac cggcccgagg agacggcggc
    11941 cgagtagctc atcgctcagc cgccgatggt cgacgacagc gcccgccggc cgagccacag
    12001 cacgagccac gccaacccca acccgaccgg cagcgcctgg atcacgaacg ccagcccgcc
    12061 gacgcgccag cggtcgcgca gccggcgccc gccgccggcc gcgatcagcg gctcgagcgc
    12121 cgcgtcgtag cggaactcgt tggcctgcaa ggcgaggccc gcagcgagcg cggcatcgcc
    12181 ccgggcggcg aacgcgcgcg cctggtgggc gagcagcgcc gcgttgggcg cggccggctt
    12241 gtcgccgccg gcgaggttgg tggctagcgc gcgcagggtg tcgagcggcc cgcgcggcag
    12301 gctccaccag ccgagcagcc aggtgtggag gctcgcccac agcgccaccc ggtcggcgca
    12361 gctgcgacaa aaggcgcctt cgatgacctc gacttgcgac cgcgtcagcc tgccggtcac
    12421 cttgcgcagc cggacgtaac gcggctgggc gctgaccgtg gcgcagcgcc ggcaacgcac
    12481 cggcgcgtat ccggtctcgc gcgcccgcgc ggggcgggcc gggccggcgc gcggcgcttc
    12541 cgccttggtc tcggcggccg ggcgcgcgct cgactcgagg gtgcggcaca gcgcgtcata
    12601 ctcgcctcgc cgatgcgcat cgctgagcac gtgataggcc tcggtgagcg tgcggaaggt
    12661 ggtgccgtcg ccggcgccgc cggtgtcggg atgcatttcc ttggcgcgcg cgcgataggc
    12721 gcgcttgacc tccgccgcac tcgcctccgg cgtgatgccg agcgccgcat agtagccctt
    12781 gacgtcgccg aggctcatgc gcgcggcgcg cgcggccgcg gctcacgaga tgtacaggcc
    12841 gccattgatg tggatcgtct cgccggtgac gaagctcgcg agatcgctgc acaggaacag
    12901 gatcacgttg gccacctcct cgggccggcc gaggcgcttc atcggcgtca gctgcatcag
    12961 caggtcgcct tggaccttga gcagcggctg gatcagcggc gtgtcgatca ggccgggcga
    13021 gaccgcgttc acgcgcacgt tgcgcggcgc gagctcgatc gcgatcgagc gcgtcaggct
    13081 gagcacgccg cccttggcgg ccgaataatg agcatgctgg aacgagcccc gatgcgccgc
    13141 catcgaggcg atgttcacga tcgcgccgcc gtcgcggatg atgcgctgga tcgcgcggca
    13201 gaagtggaac gcgccgtcga ggttgatggc gatggtctgg cgccattgcg cgtcggtcat
    13261 gccggcggtg agctggtcga gatagaggcc ggcgccggtg atcaggaagt cggcgctgcc
    13321 gaaccgctgc tcgcacagcg cgacggtggc ctcgaccgcg gccgagtcgg tgacgtcgca
    13381 cccggtcgcg accacggtct tgcccgcagg gtcgagcccg gcggcgaaag cggcgatgcc
    13441 gtcccggtcg aggtcgctca gcaccatgcg cgcgccgcag ccgtggaacg cgcgcgcgat
    13501 cgcgcgggtg atgccgccgt tggcccccga catcaccatc accttgtcgc tgaaatcgaa
    13561 catcctgcct tgccctccct tgaatatgga tgcctctgcg agccgtgaca gggcgccgcc
    13621 tcagccgcgc tcctcgccga ggaacgcctc cagggcggcg cgcatcgcgc gctcgtcggg
    13681 atcgaagccg agccacagcc gcaaggcttc gacgccctgg gcgatgatcc agtcgacgcc
    13741 ggtgcaggcc gccgcgccgg cggcgcgcgc ctcgcggcac agccgggtcg gctcgggccg
    13801 gtagatggcg tcgatcacgt cgaggtcggc gcgcagcagg tctgcgggca ccggggtcga
    13861 gtcgttgtcg gggtacatgc cgaccggggt ggcgttgacc aacagggcgg cgccggcgag
    13921 cgcggcggcg agcgactcgc gctcggcgcc cgcgaccgtc accgtcgtcg cgccatgttc
    13981 aaggtccccg gccaatgcct cgccgcgcgc gggcgtgcgg ttggcgatga ccagctcgcc
    14041 atagcctgcc cggaccagcg ccaccgccgc cgcccgcccg gcgccgccgg cgccgagcaa
    14101 tactgcgcgc ccacgcgccc gcgcgggtag cgttgcgatg cagcgcagca cgccgtccac
    14161 gtcggtgttg tggccgacca gccgccggcc agcgccgacc accaccgtgt tgaccgctcc
    14221 ggtggcgcgc gccgagccgg cgagctcatc gagcatcgcc accacctgcg gcttgaacgg
    14281 gatggtgatg cacagcccga tgcagcccgc ggcgaccacg cccgtgagcg cgcgctcgag
    14341 gtcctcggcc gcgggcgaga atggcacgaa caccacgtcg aggcccgccg cctcgagcgc
    14401 ggcgttgtgg atcagcggcg acagcgcatg gccgatcggc cggccgaaga tcgccagcac
    14461 gcgggtcttg ccggtgatcg gcgggcgcgc cgcggcgctc atgtcattat ccggccgccg
    14521 gtgaaggggc gcttgatccg gtcgctgacg ggatcgaagg cgtgatcggg gttccgcggc
    14581 atggcgcgcg gactgtgcca ccgatcgggg agtatgtcat ccggtccgcc cgacgcgggc
    14641 cggtcgccgg tcgccggtcc gatccgccac gctatattgt gctcacgcgg agctcgccat
    14701 gcccggttcg tcgcagcaat cctggagcct gatctatttc gccatcggcg gcgggttcgc
    14761 gctcgcgttc ctggtcgcca tcctcgcgct cagccgggcg cgcgcccggc cgtattggct
    14821 gttcgcggtg ctcgcggtgc cggtcgtcgc cctgttcgcc aatcccgact ggcgcctgta
    14881 ttccgcccac gcgctgtacc actacagcat cgtctaccag attttcgagc atggcgtgcc
    14941 gccgtcgctg ccgctggccg cgggcgtcga cctcaagtat ttctatgcca ttcatttcgt
    15001 catcgcccag ctgatgaggc tgttcgcctt cccgcccgcc tgggcgttcg cgaccctggg
    15061 cgtggcgtgc ctgttggcga tggtgctggt gctcgaccgc ctggcgcgac gcgtctcgga
    15121 cgacattccc taccgctggc tggtgctgac cgtgatcctg ttcggctcgg cggtgctggt
    15181 cggcgatccg atcggccgcg ccctggcgaa cacgggcgtg atcgtacgcg aggggcgcct
    15241 cgacctgctg cacaagttca tcgacatcaa caacaaccag ctcggcctgg tgctgtacgc
    15301 gctggcgttc ctgggcctgg tcaacctggt gggcgagggc gggcgcgagc gcgccggcct
    15361 ggtccagttc gccacgggct acgtgctcgc cggcgtgttc tatccgccgg cctggctcgg
    15421 ggtgtgcatc gcggcggcgg tcgcagtcgg ccagatggtc gcgctcacgc ggccgatccc
    15481 gtggcgcaag atcgcgcaac tgtcggcgat cggcgtggtc ggcctggcgg tgctcgcgcc
    15541 gtggctcctg tcgctgaccg tcggcaagtc cgccgacagc gcgatccgcc cgaccctcgg
    15601 cctgctcgcg cgcaacgcgc cgatcctggc cgcggcgctc gccgttcccg tgctgctcgc
    15661 gctttacaag tcggagcttc tggtcaggca ctttcgggag cggccgcggc tgttcgggtt
    15721 tctcgcgctg tcgatgatcg tgctgcaggc ggcgttcctg ctcgtctacc tgccgaccga
    15781 tgccgagtac aagatgatca acttcgcctc ggtgccgctg ggcttgatgt tcgcgctggt
    15841 gctgcgccag atgttcgacc aggtgcgcgt gttggcgacc gtggtgctgt tcctggtgct
    15901 gctgccgttc agccagaaga tcggcaccta tctggtgttc ggctggccgg tgtcggaccc
    15961 ggcgaccacg gcgggacgct acattgtcga ccgggtgccc gcgcagcagg cgctctacca
    16021 gtggatcgcc gccgagtcgc ccgacgacgc ggtgttcatc gacagctacg tgaccgtgcc
    16081 ggcgttcgcc aggcggccgc tgctgatcgg caccgagttc cgccgcgagc gcggcgagct
    16141 ccacgtcgcc ggcgagatgg gcttgcgcaa ggacggctgg ggacctgtga tcgacctcat
    16201 gttgcagatc aacggcgtcg atcccgacct cgccgcgcgg cggcttgcca tcgccggcga
    16261 gttcctggcc gagaccgacc ggcggttgtc cgacgaggtg ttcgccgcct tgcgcggcga
    16321 ggtcccgggc cgccccgtct acgtcgtcgc gcgccacggc gcggtgatca cccggctcgc
    16381 ccgcgaccgg cgcttgacct cggtgttcca gaaccgggcg gcgacgctgt ttcggatcgc
    16441 cgccgccggc tgacgggcga acgtgaagcc cgacacgctc gcgcgtttcg ccgcgccgca
    16501 ggggatcgga gtcggccgtg ccgcccgccc ggcgctcgcg ctcgccggcg tggtcgccct
    16561 tgccgcggtc gcccgcatct ggggtctcga ccggcattcg ctgtggctcg acgaggcgtt
    16621 cagctatcac tactcgggcc tgtcgctgca cgacatctgg cgggcgatgc cgctctacga
    16681 gagccacccg ccggtctact tcacgatcct caaggcctgg cgggcggcgt tcggcgacgg
    16741 cgaggcggcg atgcgcggcc tgtcggtggc ggccggcacg gccacggtgg cgctggtcta
    16801 tctgctcggc cgccttgccg gcgggcggcg agacgggcac cggatcgggg cgttcgcggc
    16861 gctgctgttc gcgatctcgc cgctgcacct cgacttcgcc caacaagcgc ggccctatgc
    16921 cctgatcata cttgccacgg caaccacctt gtgcggggtc gcctggctgc tgcgacatcc
    16981 ggcggtcgcc cgctcgccgt ggctcggcct cgcggcgatg cgcgcccacc cgcggcgcgc
    17041 gccgctgctg cgccgcggcc tcgccgcctg ggcggcgacc gcgctcggcg ttgcggcggc
    17101 actgtggctg cacgacaccg tgctcgtgta tgcggccgcc ctgggtttgt tcgggctgtg
    17161 gctcgcctgg cgctggggct ggcccaggtc cttcgtcgct aacctgatga tcgcgggcgc
    17221 cgccgcgctg gcgctgtggc tgccgttcct gcccaccttc gccgtccagc tcgccggcgt
    17281 gtcgcgtgcg ttctggatgc ctgacctgac ctggcaggcg gcgctggcgc cggtcggctt
    17341 cctgatggac gtccgcttcg tccgcccgct caccgcggcg ccggtgctgg tgttgctggc
    17401 gctcgccggc gcccgcggcg tcgccggccg gtcgggggcg ccgatggccg cgctgctgat
    17461 cggcgtcgcg gttgccggct acggcctgat gctgcttttc tgcctgctcg tgcagacagt
    17521 gctggtcgag cggaccctga tctggatgag cgtgccgttg ctggtgctgg tcgcggcggg
    17581 cgtggtcggc ctcgagcggc gggcgctgcg cgccgcctcg gccgtggtcg ttctcggctt
    17641 ctcgatctgg ggcgcgctgg tctttcagct tgcctatcag cgcgagcctt ggcgcgacat
    17701 cgccgcccgc atcggcggcc aagccgtcgc cggcgacgtg gtggtcgtga tgcccaacag
    17761 cctggcgctg ccgctcgcct actatcgcga ccggctgggc gatcaggtcg cggtgcacgc
    17821 catgcccgcg gcgttccccg agatcagccc cgaccgcgcc tatccgacca gcgtgctggc
    17881 cgagcccgcg ttcgtgcgcg cggacctgcc cgcgctcgat gcggcgatcg cgggcgccgg
    17941 cagcgtctgg gtgatcacgc gcctggacga tatcttcgat cccgaccggc tggtgctcgg
    18001 ccacatgcgc caagtcagga ccgagaccga gcactggccg gtcagcggct atctgcacgt
    18061 ccacaggttc agataggcgc ctggtgggcg gcatggccga gatcgtgaca gcgggcgcaa
    18121 gcgcgcgccg gaccaccgtc gcgctggcgg ccctggtcgc cgtcgcggcg gcgctgcgcg
    18181 cgtgggggct cgagcggcag tcgctgtgga tcgacgaggc ttacgcgtac tggttcagca
    18241 gccagtccct gcacgatttg tggcaggtct ttcccgccca ccacaaccac ccgccgctgt
    18301 tcatgaccat gatgaagggc tggcgctggc tgctcggcga cggcgaggcg gcgatgcgca
    18361 gcctgtcggt ggtgccgagc gtgctgaccg tgccggtggt cttcgtcatg ggccggctcg
    18421 ccggcggggc cgcgcgcggc gcgcagaccg ggctgctcgc ggcgctggtc ttcgcgctgt
    18481 cgccgttgca catcgactac gcgcagcagg cctacgtcta cacctggttc accctcggcg
    18541 ccggcctgtc gctcgccggc gcattctggc tgctggttca tgctcgcgcg gcggagcgcc
    18601 cgtggctcga cctgcggcgg gacacgcacc gtgccggcga ggcgcgggca accggcgcct
    18661 gggcggcgac ggtgatcggc tcggcgctcg cgctgtggat gcacaatgtc gccgtcgtct
    18721 acgtcgccgc gctcggcctg tcggcggcct ggatcggctg gcgcgacggc aggccgcgct
    18781 ggtatttcgc caacctcgtc ctggccgggc ttgccgtgct ggcggtctgg tcgccgttcg
    18841 cggccaagtt cgcggcccag gtcggggtcg tctcgggaaa cttctgggtc gaggacgtca
    18901 ccctcgcctc ggcgctgcag ttcatcccgt tcctgttcga gatccgcttc acctggccga
    18961 tcaagggctc ggtgctgctg gtcctgctcg ccgcgctcgg cctgttcgcg atctcgcgct
    19021 gggggcggcg cgacctgacc cggctgatgc tcggcgtggc cggcctcgcc tacgcgctgc
    19081 tgctcctgta ctcgctgctg gtgcagtcga tcctggtcga ccggctgctg atctgggtca
    19141 gcctgccgtt ctatgtcgcg gtggccgcgg gattggtcgc gatgcgccgg cgctggctga
    19201 gggtgctgct ggtggtcgcg atcgtcgccc tcagcctgca gggctacgtc aactaccaga
    19261 ggggattcga gcgcgagccg tggcgcaaca tcgccgctca cctcgccgag catgcaggcc
    19321 ccgacgacgt ggtcgtgaac ctgcccaacg acaacgtgat tccgctcgct tactatctcg
    19381 acgccggcgg tggctcgctc aagctcgtcg ggctgccggt gccgtacccc tccttcgatg
    19441 agagccggtt gagcccggtc ggcgtgatct tcaccccggc ggtcgaggcg gcggacctgg
    19501 cgcggctcga cgaggtgacc gagggggcgg cggcggtgtg gtacgtcgcg cgcgagaccc
    19561 gcctgttcga ccccgaccag atcgtgctcc accatttgca gaagacgcgc cggctcgcct
    19621 ggcattggcc ggtgcgccag cacatcgacg tctactgctt cgagtgagcc tccgccgcga
    19681 acccggcctc cggcacgtag taggcggaca tcgccgccat gcgcacggga cgctcctcgc
    19741 ccgggaccgg gtgggtgttg acgtactccc agacgaccgt gccggaggcg aggtcgatct
    19801 cgacgatctg gccctggtcg gcgagcgcaa ccagggcgcg gcggccgtcc gggtgcaggt
    19861 cgatatgccc ggcgaacacc gttgcgaacc ggcccgcgcc gctgccgccc ggcaccggat
    19921 agacggtgcg caccgcattc gtcgccaggt cgatctcgac caggcggctc aggttctcgg
    19981 ggtcaagccc gcccttgttg tcgaaggcga ggactttgtt cgcgccactg aatcgcgggc
    20041 tgtgctgcgc cagcgtccgc ccggcgagca gccacttgac ccggcccgag gtgccgtcga
    20101 gcaccgccag cgcgttgctg gtccgcagcg aaaccaacag gtcgcccgcg gcgaggcccg
    20161 gatattcgga cgcgagccgg ggggaaagca cgcgcacgtc gttgaggtgg gtcgggtcgc
    20221 actgctcgga ggcgagcgcc tccgcgtgat gaagcaggcc cggataatcg gccgcgagca
    20281 gggccttcag gaccggcagc tccctgagca gccgcccgtc ggcgtcgagc accttgatct
    20341 cgtcgtcgta gacgaagccg gtctcgcagg tgatctcgac gccgccgccg agcgctaccg
    20401 gcgccggcgc caatcgttgc gacggcacat aaatgcgccc cccttcgtcg agcgagaacc
    20461 agtgatggct gaggttctgc tgccgccata gcagattccc ggccttgtcc agcttggcaa
    20521 tgccgatggc gtaggggaat gcgctcgagc tctggtagga gacgagcagg tcgccgttcg
    20581 cgaacaggtg caagcccatc ggcaccagcc ggtcgagcgg cccggccccg gcctcgcgcg
    20641 cgcgctcgcc ccacagcagg tcgaggtcga gcggccaggc gtgcacgacg cgcccttgcc
    20701 ggtcgatgat ccaggcgagg cagccctgct ccgggcactc gctcatgagc tcgtaagggc
    20761 cgcccccgac caggatcagg tgatcgtttt cggtagcatt tccgtgtgca atgacagttg
    20821 gttgtgagta gtccttatca tatcccatga acacagtcgg ctgcccgacg ccgagcgcgg
    20881 gaagaagcgc ccggagcgcc aggcgggcgt cccggaactg ccggtacggg aacacctcga
    20941 aggtcgtggt cgtggccgcg ccggcgatga aggcgagcgc gcacaggccg atcaggaaca
    21001 ccgcgcgcgc catccgccct cgctcgtttc cggcggcgtc acacagcgcg cgcgtcgccg
    21061 gccgcgtgcg cggcatcggc ggcgtatccg agccgccgcg cgaccgcgtc cgtcgccgcg
    21121 gcgatcgcct gccgctcgtg gtccgagaag tcgggccggt aatagtccgg gcgatgcagc
    21181 cggcgggcct gggcctcgac cagtgccgcg ccggcctcca gccggcaatg cgcatacacc
    21241 gctcgcagcg tcgtctcggg ccggtcgcac aggtcctcgt agcggacgat catggtcgcc
    21301 tcggccagac ccgggtgggc gccgatgcgg tcgaggacga agccgtacac ctgcgcccat
    21361 tgcagcgcgt agccggcggc gtcctcgccc gcggcccaca ggcggcgcac ctcgtccgcc
    21421 gcgccgcgat cgccgagctc gagcgcgcgc cggtcgagcc cgaactcgaa atgccgcagc
    21481 ctctgcatgt gcgcgaggat gcgcgcatcg cgctgctcct cgcggcagaa caggcggtgc
    21541 tgcttgacca gtgacgcgac atgggcctcg gggtggcgga cggggatgac gaagcgcgca
    21601 tcggggaaca gctcgtagag gtagcccagc cgggcgacgt ggtagttgtt cttcgccacg
    21661 taccggcggc cgccgcgcac cagcaggatc ttgcgcaggt ggtcgcggta gaagcggtcg
    21721 aacgcggcat cccggtccgc gaagcgttgg ttccagagca cctcctcgaa cgcttccggg
    21781 ctttcgggcg tgaccagcag gccgtccttg tgcgcccgct cgacgggctc cgccgcgcgc
    21841 cgcgggacca gctccaggaa cgcgttccac cacagcggca cctggatcgg cgggacgtcg
    21901 cggtagctgt gggtcgccac ctcgggatgc gctgccagca cctccaagag gatcgtcgat
    21961 ccggcgcgcg cgagcccggt cacgtagatc gggccgacga tggggatcgc ctcgagccgg
    22021 gccgcgtacc atcggctttc cagcgccgcg agacgcgtca ggaggccgtg cgcgcgctcg
    22081 acgagggcgc cgaaccggtg gcggctcgcc gacatctcga tggcgttcgg tggcgtttcg
    22141 gacatcgacc gcgtgcgtgg gacggtgccg gcggcaaccg tgggccggac ctagagccca
    22201 atctagcccg gtcccggccg atgtccacgc atgctgggcc gcgcggtcct cggcggctat
    22261 catcgcggcc ggcgagcgac aacggggagg agacgatggc ggcctatctg atcgcgcaga
    22321 tcgaggtgac cgaccaggag gcgttcgatg cttaccgggc caaggtgccg gcgacgatcg
    22381 cgaagtatgg cgggcgttac ctggtccggg gcggggcgat cacgacgctg gaggaaagcc
    22441 cgcccaagcc gcgcgtgatc gtgctcgagt tcgagtcggt ggcggcggcg aggcgctggt
    22501 acgagtcgcc ggagtatcgg ccattgatcc cgatgcgccg gagggccgcc gacgggccgg
    22561 tgatgatcgt cgagggcgcc tgatcggcag caacgagggg cgcttcccgg cttgagggaa
    22621 gcgccccgtt ccgctgcgcc cgggccctgg cccggcgctt ggttccgtgc tcaggcggcc
    22681 ttgccgccct cgatcacctt gtgcgtgccg gcctcggcgc cgatggcgat cttgcgcggc
    22741 ctcttctcca cgggaatctc gcgcgacagc tcgacatgga gcagcccgtt ctcaagcttg
    22801 gcgccgctga ccttgatgtg gtcggcaagc tggaacctgc gctcgaacgc gcgcgtcgcg
    22861 atgccgcggt gcaggaaggt gtgcccggcg tcctcgcccg cgatgcggcc ggagaccacc
    22921 agcatctcgt cggtgacggt gacgtcgagc tcgtcctcgc cgaagccggc caccgccatg
    22981 gtgatccggt aggtgtcctc gtcgagcttc tcgatgttgt agggcggata gccggtgccg
    23041 ccgtcgaacg cctgcgacgc ctcctcgagc atccggttga ggcggtcgaa gccgacggtg
    23101 gtgcggaaca ggggggtaag gtcgaaggta cgcatcacat atcctccagt gagcaatatg
    23161 ttgcctgggg cccgccgacg gcgcagcccc ttacgatcat tgcccgcggg cccgaattgg
    23221 cgcccgccgg cgatcttcag ataagcatct cggcgtcgta ttcaagggtt cggacgggcc
    23281 aattcgggcc gcttccgccg gcctcttcaa aacggagtac aatcgccatt acattgtgta
    23341 attatataag aatataaggc gtatttaata tattagaata agttaaatcc gagcggtttc
    23401 tccgctccac ccgaggccgt gcggcaacgt accggccgga ccggagttgt cggcctggac
    23461 acggactccg cgtgcgggca aggtggcgcg ccccgacgtc ggggccaaga ccggaacttg
    23521 cgggaggggg cagttggcaa gaccgttgcg cgccatgatc cgccggcgcg agcccgcgtt
    23581 cggcaccttc gtcggcgagt tcgacacccc gggcatgggc cacatcctcg ctgccgcggg
    23641 ctgcgacttc gcgttcctcg acatggagca ttccgggctg accttggcct cggtcaaggg
    23701 cgcggtgcgc cattacgagg ccgccggcgt ggcgctgatc gtgcgcccgc cgtcgaaggc
    23761 ctacgaccac atcgcgcggg tgctcgacac cggcgccgag ggggtgtgcc tgccctgcgt
    23821 cggcagcgcc gaggaggcgc gcgcgatcgt cgcttccgcc aaatacccgc ccgcgggcga
    23881 gcgcggcatc ggcctccagc tcgcccacga caactacgtc cgcggcgagg tcgccgccaa
    23941 gctcgccgcc gccaaccggc ggagctgcgt ggtgctgcag atcgagtcgg cagaaggcgc
    24001 cgagaacgcc gacgccatcg ccgcggtgcc gggggtcgat gcgttgtgga tcggccacta
    24061 cgacctcagc gcctcgctcg gcatcgccgg ccagttcgcg catcccgatt tcctggccgc
    24121 ggtgcgccgc gccatcgacg cctgcaagcg gcaccggcgc gggctcggca tggtggtcgg
    24181 ctcgcctgag cagggcctcg acatgatcgg cgccggcttc aacctgatcg cctattcggg
    24241 cgacgtgtgg ctgttgcgcg acgcgctcag cgccggtatc gatgcgatgc gcgacggcgc
    24301 ccgcgtgcgc ctgggcgcgg aaaccggcag gggcaaggca tgagcaagtt ccgggtcgcc
    24361 atctcgggcg actcccttgc ggtcgcgctc cccggcgatc ccgtgaaata ggcgccggcc
    24421 gcacgcgtca gtcgcgacaa tgatcgagcg accgcgtcac ggcgccagcg gttcagcgat
    24481 cgatccgccg tccgcttcca tcaggcgcgc ggccgcgatc gggtgcatcg atgcaccagg
    24541 gctccatcgc cgtgcgcacc acgagcatta ccccgaacag ctccgggttg aagatctggt
    24601 tggccgcggt ggacaggatc ccctgctcgc agtagtgcgg cttcgcgccg tctccggcgc
    24661 aggcgcaaag gagtgctacc gacagagtga tcaagacgcg acaattcatc gcccgcgcgc
    24721 tgctgcacga ccggtccgcg acctcgaccg gcgggcctgc ggcacacgcg cgccggactc
    24781 ccaaggcgcg ctacccgcat acatgatcct cgacgtcctg ggaggcgagg ccgatcagga
    24841 atcccatcgg cgcgaaaaac accccggcga ttgccatcag ggggtagttg gcaggattgc
    24901 atgcgaacac gtcgccgttc gcacacgcct cggccatgtc gtccatggtc gcgaagaaac
    24961 ccatcgagaa ctcggccgcg gcctcgaccc ttccgcagga ctccgcctgt tgcggggagt
    25021 cggccgcaat gctcgcggtg ttaggctctg tgctggcgca ccccgagacc atcaaagccg
    25081 aggcgagaat tgaaaggcca aagcgagtcg tcgtgaaaag ctgaatcatg gcagtcctcc
    25141 atttcaactt ggatcgccgg gttgcactgc aatgcaggac tcatccagca catcgcgttc
    25201 tccgccgtcc tcccccatct catgccgcac gccggccgcg gctacaagcc atagcgaaga
    25261 gaccatcgaa ctgtcatcgt caagccgggt cggtgttatt cgctgatgtc atggcctttc
    25321 tcgcgaagac agtccttgaa cgtctcctca taagcccgat cttccgcgga cgccgcatac
    25381 tctaacgtga gccaggcgcc gaggccgccg gtcagaatcg caatcgtctc tgcctcgtct
    25441 gacatttcgg caaccctgtc gaaggcggct ctacgggctc gcgcttggca ttcgctcttg
    25501 tgaacttcag agatgtcttc cgggggtgaa taggagaacc gctcatcccc ggcgggccgc
    25561 gcaaaggcgc aaccgctcac ggcataggca gtcagaacaa ctgcaatgcc acagctaagt
    25621 cgcgtcatgc catgaccaac cgaggaacgc aagcgcagcg ctcggggatg tcccgcgcag
    25681 cctgaacgca actgcttggg ttgactctcg tgacattgca acatgtttgc tacatccttt
    25741 tcccgaggac gacttctccg gtatccgcgt cctccatcca tacgaacgac gtgtacccag
    25801 ggtgggacca acagaaaatc ggaaagcacg tgtcctcctc aacgttgtag gttcggcccg
    25861 ccaataggtt gacttcacca actgccaagg cagtggtact ctttcgtccc ctgtgtcgaa
    25921 ggctgatgta gtatgtaccc ggcagaagca taatcttatt ggggaaatca aacgtgctgg
    25981 ccgccccgga cggtgccgaa taaatttttt tgctctcgga atttatccat gtcacgcaac
    26041 tccagcaccc cgcaccctga tcgatgattg cgacctcgtt ctcggggcga ggtgtgccgt
    26101 cgtaggcgct caactccggg ccggcgtgat tccagtacac tccgcaaccc gacacgcaca
    26161 gggtcgcgag gataagaacg ctccttgaaa gggccgagcg aagcattatt cacctcctga
    26221 cagtcgccgg gccgtcggcg ctcgaagcgg cttcgcgagc gatgagcggc cggcgattaa
    26281 ggcgatggcc aagggccttg cgatcgagcg gttcacggct gcgccccacc ataaggtcgg
    26341 tcgctggtgc gcaattccgc cgcgatctgg tcgtcggcga tcacggcgga ctgcttctgc
    26401 tgctcgagct cggcgagact ccggtccgag gccgccttca gggcggcctt gttgtcctcg
    26461 cagtcgttcc tggtctggcg gccagccgag gcttgcgcgc agctccggtc aaattgctcc
    26521 tcgacgagcc tgaacttggc ttcgatgtcc tcggcgcgcg tggcaaagaa ccggtcgcgg
    26581 gcctccgcgg tggggaagct cgcaggatac aaggcaaatc gaaccgtctc cggatactcg
    26641 ctcatcgagc ccgaggccgc gtcgacgacg aggccgactc cgccgccgag gagcccgaac
    26701 agaatgttcc ccgccgccca gccggaggtg cccgaggcaa cggtctggcg ggtatccagg
    26761 tagccgtcgc gggtgcaggc gatctcaagg ccattggcgc tcttgctgat ctggatcgtg
    26821 ccgggcgtgc tgctcacggc gccgaccatc gctccgtcgc gcgagatctc gcatttggcg
    26881 cggcccgggt cggtggcgac cgtaaagggc tgcgtcgacc ctttgaccac cgtcgcgcag
    26941 gcgtgcagga acggggcggc aaggatgacg agcgcgacgg cgaggacatt ggtttgccgg
    27001 ctcatgatgt cggcctcctg ttgtcggacg gcccgcaggc cacactggga atcccgaaat
    27061 ggcgccgctc gctgcgcgcg tccggatcga cgtcgtcggc cacgtcgtca tgcagggcgc
    27121 cgtgcgcgtc gctgaccatg gtcgggcgga agtacgcctc gaagcgcgcc ggcaggcgca
    27181 tgaacggccg gtacagcgga tcgagctcct cggtgctggc gaggtaggtc cggcgcgggc
    27241 ggcgcagccg cagcctgagc ggcgaggacg cgaacgtctc gttgctgatc aacgcgctcg
    27301 tgaccatggc ggcgaacagc tgcagcggcg tcagccggcg ccgatagtgg cggaggaaac
    27361 ggaccgcgtg tctcagcacg cgcgtgcgat agccgcgcaa ggacggcagg tcgcgggcga
    27421 accctaccgc ctcctcaagc cggtcgagcg accgcagcgc caaggtcact ccgtcgagat
    27481 cgcgcaacct gacatgcggc aggagccgat gctgagccaa gcaatcgtaa aagtacggcg
    27541 tgccgagcag agggatggac agggtgaagt acgccggcag cgtgatcgtc ggcgtgtcca
    27601 gcagcagccc gatctcggcc ctcagcttct cgaggcggcg tacgcttgga tcgagcatga
    27661 tgccgtagct gaacagcacg cccgcctcga ggcaggttcg gatcatctcc agctggggca
    27721 ccacggtgct gtggcgcttg ttgtgggcgc gcagggtctc cgcgtcgagg gactcgacgc
    27781 cgctgaacag cgactggcat cccgcctcgc gcaccagatc gaggttctcg cggcgggcga
    27841 agaagtcgcc cgtgaccagc gccgaccagc cgtcgatccg tccgctgcga tagaagtcgc
    27901 gaagcagggc gacgcggtcg aggaagaacc tgcggtcgtt gccatagaaa ttgttgtcga
    27961 tcaggacgat ctgctttttg cctaccgcct cgatctggcg cttcaggtag tcgatctcgt
    28021 agtgccggta acggccgcct tcgcccgtga gcgagcagaa gccgcagcgg aagttgcagt
    28081 tgcggctcga ctcgacgtaa gcgaccaggc ggtgcgggtc ggcgagatcg aagcggggga
    28141 acatctcctc ggcgacgtgg gcggggccga gcgcatcgcg cgccacctcc tgaagggcct
    28201 cgatgtcgcc ggtgcacgga tagtcgaaga agcgcgccgc ccgccggggc agggcgcgga
    28261 tcgccggccc gccggcggcg acgatcacgc gcggattgag tgtccgggcg taagcggcca
    28321 gatgcagcat ccggtcgaac gacgaggtca cgcccgtgag caccagcaga tcgggcgagc
    28381 cgagtagggc ggcgtcgtgg agcgggccgc tgtattgctc gttgtagaga cggacctcag
    28441 tggtcgagcg ctcgaacgcg cccgccagga agacgtgtcc catcgcgcgc ggcacgcggt
    28501 gcgggctgcc ggccgtccgc cggtactcgt cgaagaaggc gttgaccacc agaacgcgct
    28561 tgcccatcgc acctcacccc agttcgcgcg tcgcccatgc ccggccggcg ccacttgcca
    28621 cgtgcggaga gtcttcccgg gcgccgtcat ggtggcgtac gctggcgccg ccgcacggcg
    28681 tcaaatcctc gtcagggcgg cgcaaatccg ggtcaccgcc gcgcagcgtc ggcactcgcg
    28741 ggccgagaaa ccccgtttgc ccaagccccg cccgggggta agcttgagcg ccgtcgccga
    28801 agggcgcgag acaaggggtg gcgaatcacg tggcgatagc gggcgtttcc gtacggctgc
    28861 tcggcaggct cgaggtccgt tcatgcgagg gggccgagat ccccgtgccc ggtcgaatgg
    28921 cccaggcgtt tctcggttac ctcgccgcgc attcggagct ggctgagggc cgcgagaccc
    28981 ttgccggctt gttctggggc gaccgctttc gtgaccaggc gcgccacagc ctgcgccaga
    29041 ccgtgctggt gttgcgcaag gctttcgagg atgcgggagc gcaggcgctg gcggccgacg
    29101 cccatagcgt cgcgctcgac cccgcagtcg tcacggtcga cgtgcagcgt tttcgggcgc
    29161 tcgctgccag cgagcaacgc gccgacctcg acgaggcctg cgccctctat cgcggtgaac
    29221 tcttggccga cgtgtcagtg cgagcggacg gcttcaccac ttggctcgac cgcgagcgcg
    29281 agcagctgcg acagctggcc gcggacgcgt tcatgcgcct cgcccggctc gaggccgcgg
    29341 aaggcgccca cgatgccgcc attgccgccg ccactagggc gctcgcgatc gagccggcac
    29401 ttgaggactg tcatcgcgag ctgatgcgcc tctaccggct tcagggcaac cgatcggcgg
    29461 ctttgcgcca gtaccaggca tgcgtcgagg cgttgcggcg cgagctcgat gtcgaccctg
    29521 acccggccac ggtccgtctg gccgacgaga tccgggccgc cagggacacg ccgccgccga
    29581 ccgcgaccgg cgggtcgacg cagcccgccc agccgtcgca gtggcgcact tggctgccgt
    29641 cgcgccgttg gcggcggctg tcgccctacc tcgccggcgg cctgctgctg ctgctcggcg
    29701 cgatcggcgg agtggcgcta ctgaccggcg tcgagcgcgt gcgcgaggag gcgcgccagg
    29761 agcggatgct tttcccgctg ccggcgcagc cctcgatcgt ggtcttgccg ttcaagaacc
    29821 tgaaccgcga cccggcctat caggtgatgg tcgacggcat gaccgaagag atcaccgccg
    29881 cgctgtcgat ggtctcggag atgttcgtga tcgcgcgcac caccgctctc acctaccgcg
    29941 accgggcggt cgacgtgcgc gaggtggccg aggagctcgg catccgctac gtgctcgacg
    30001 gtgcagttca ggtcgaggag gaccgcttgc gcgtgctggt cgagctggtc gacgcgatcg
    30061 agaaccgcca ggtgtggacg caccgctacg agcgtgagat gaccagcctg ttcgagatca
    30121 acgacgacat taccctcgag gtggtcaccg gccttcaggt ccagattacc gagggcgagc
    30181 aggaccggat ctccttggtg cacggcaccg acaacctgca agcctggctg gtcgccgggc
    30241 gcgccctcca gcaccttagg cgcctgacgc gcgaggacaa cgcgcgcgcc cgcggcctgt
    30301 ataggcaggc gatcgccctc gacccgtcct atcccggcgc ctacgacggg ctgtcctgga
    30361 cctatttcat cgacgcccgc ttcggctgga cggaacggcc cgacgaggcc attcgccagg
    30421 ccggcgagct cgctcagcgc acgctcgagc tggacccgga gcggccggcg acttacgccc
    30481 tgctgggggg cgtccagctg atgctcggca accacgacca ggcggtggcg ctggccgaga
    30541 aggcggtcga actcagcccg agcggcgccg acgtgaccgc gatcctcgcc ttgatcctga
    30601 cctatgtcgg cgactaccgg cgcagcgtcg cgctgtttca acgcgcgatg cggctcagcc
    30661 cctcctatcc cgactggtat cgctggtcgt tggggcgggc ctatcggctg ctcgggcgcc
    30721 atcgcgatgc cgaggccacg ctcgctttcg aggcgacgga agccggcggc tcgatcgcgc
    30781 cgctggtcga gctcgccgcg acctatgccg agttcggcaa gatgcgcgag gcgcgccagg
    30841 tcgcccggcg ggtggcggcg gtggcgcccg gcttttcggc gaccgcctgg acgcgcatgc
    30901 cgccgcaccg cgaccaggcg accgccgccg ccgaacgcgc cgccctgatt cgcgccggac
    30961 ttcccgagta gtcctagcag gccgggggct tggcgatccc atcgcccttc gagacgccgc
    31021 cacacgaatc gtcacatgca cggtgctcgg ctgttgcgcg acgcgcccgg cgccggtatc
    31081 gatgcgatgc gcgacggcgc ccgcgtgcgc ctgggcgcgg aaaccggcag aggcaaggca
    31141 tgagcaagtt ccgggtcgcc atctcgggcg accttctcaa ggacgacggc actccggcga
    31201 tccccgagtt cgacctgtcc cggctcgagc gcgaggccga cgtcgagtat gcctacgtgg
    31261 ggcgtggccc ggtgctcgat gcgggcgagc tcgaggactt cgacgccctg atcctgatga
    31321 tgccgcgctt cgcgccggct agcgtgccta agagcggccg cctggcggtg gtcgcgcgcg
    31381 gcggcgtcgg ctacgacacg gtcgacgtcg ccgcctgcac cgaggccggg atcgcgctgg
    31441 tcatcacgcc cgatgcggtg cgccggccgg tggcggtgat ggtgatcacc ttcatgctgg
    31501 cgctgtccgg gcacctgttc gcgctcgacc gcatcacccg caaggggccg tggcgggcgc
    31561 gcaccgacta catgggggtg gggctgatcg ggcgcacctt cggccagatc ggcatcggca
    31621 acatcggcgc cgagaccttg cggctggcga ggccgttcgg gctcgagctg atcgcccacg
    31681 acccgtatgt cgacccgtcc atcgccaagg acctcggcgt caggctggtc gatatggaga
    31741 ccgtgttccg cgacagcgac ttcctgtcgg tgagctgccc gctcaacgac gagacccgcg
    31801 gcctggtcaa cgcccggcgg ctcgcgctga tgaagccaac cgcgttcctg atcaacacct
    31861 cgcgcgggcc ggtggtcgac cagagggcgc tgaccgacgc gctcaggcgg cgggcgatcg
    31921 ccggcgccgg gctcgacgtg ctggagaagg agccgccgga ccccgccgac ccgatcctca
    31981 ccctcgacaa cgtcatcgtc accccgcacg cgctgtgctg gaccgaccag atgttcgcga
    32041 ccatggcgga gaaggacatc gacgcctgcc tcgccgtgat gcgcgggcgg gcgccgcaga
    32101 acgtggtcaa caaggcggtg ctggattcgc cgcgttttac cgacaaactc acggcctacc
    32161 ggaacgcatt cggcaagtag ggcgccggct ctcgttgagg cacgagttgc aaacctcatg
    32221 cttcgagacg cccgctttgc gggctcctca gcatgaggtg aattggagtc aagggtgttt
    32281 agagcctcat cctgaggagg gcctcaggcc cgtctcgaag gatgaggcgg gcaaccgcat
    32341 tttgtgattc gaagtatcgt ggtgcagatg agcggcctgc gcgacctgac gccgttgctg
    32401 aagccgaggt cggtggcgat catcggcgcg acgcccgacg cgcgccgccc gggcggccgg
    32461 ccgctcgact acctgcgccg gttcggcttc gccgggccga tctatccggt caacccgcgc
    32521 tatcgcgaga tcgccggcgt gcgctgctac gcgagcgtcg ccgacatcgc cgaggcgccc
    32581 gacatggcgg tggtctcggt gccggcggcg gcgaccctgg acgcggtgcg cgactgtcag
    32641 cgcgcaggcg tgccggcgat gaccatctac acctcgggct tcggcgagac cggcgcagaa
    32701 ggcaaggcgc tcgaggccga gctgatcgcg ctcgccgagg ccaaaggcac gctgatctgc
    32761 gggcccaact gccaaggggt ggccaacttc cacgaccgca tggtcgccaa cttcacctcg
    32821 gcgctcgcca acgaggggat cgatgccggc ggcatcggct tcgtcagcca gagcggcctg
    32881 ttcgccggca tcgtcaccgc cgagttccgc cgccgcgggc tcggcctcgg ctatctggtg
    32941 tcgagcggca acgaggcggt ggtcgacttc gccgacgtgc tcgcgttcat ggccagggac
    33001 gagcgcatcc gggtggtcgc cggctacctc gagggcgtgc gcgacgggcg caagctcgcc
    33061 gaggcggcgg cggtggcgcg cgagttagcc aagccggtgg tgctgatcaa ggtcggccgc
    33121 tcgcaggaca gcgccgcggc tgcggcctcg cacaccggtg cgctcgccgg cgcctacgcg
    33181 gtctacgccg ccgcgctcgc ccagtggggc atcctcgagg ccgactcgat cccggagctg
    33241 ttcgacctga tcgagacctt cgcggtcggc gcgcggccgg cgcgcggcga ccgggtcggc
    33301 gtgatcacca actcgggcgg catcggcgtc tattgcgccg acaaggtgcg cgagtgcggc
    33361 ctcacgatgg cgagcttcgc cagcggcacg gtcgaggcgc tgaaggcgcg cctgcccgcc
    33421 ttcggctcgg cgcgcaaccc ggtcgacttc acgctgcagg cctacaccga ccccgagtcg
    33481 gtcggcgcgc atctgaggaa cgtggtgctc gatgacggcg tcgacgtggc gctcgccttc
    33541 ttcggcatcc agctgctcaa cgtcgatgcg gtggtcgacc aggtcgcggc ggcgaatgcg
    33601 ctcaacgaca agccggtgct ggtcggctgg atgctgggcg atgcgtccgg cccgccgcgc
    33661 ctgcgcgccg ccggcatccc gtgcttcgac gacccgctgg cggcgatcaa ggcggccaag
    33721 gcgctggtcc gtcaggggcg gatcgccgcg accgcagccg cgacgcgaac gccccatgcg
    33781 atggatgcgg cagcggcgtt ggtcgccggg cacgcgcccg gcgcgctcag cgaaagcgag
    33841 gcgcgcgcgg tgctcgcggc ggccgggatc gcgccggtgc cgggcgcgcg ggcggccgac
    33901 gccgaggccg cggtggcggt cgcgcgcgag atcggctacc cggtggtgct caaggtcgac
    33961 tcgcccgaca tcgcccacaa gaccgaggcc ggcggcgtcg cgctcgatgt ccgcgacgac
    34021 gacggcgtgc gcgcggcctt tgcggcaatc acggcggccg cccgcgcgca tgtgcccaag
    34081 gcgcgcatcc gcggcgtcgg cgtctaccgc atggtgcggg gcggggtcga gatgatctgc
    34141 gggctgagcc gcgatccggc gttcggggcg gtggtcctgg tcggcagcgg cggcatcctg
    34201 gtcgaggtga tcgaggacac cgccctcggg gtcgcgccgc tggaggaggc cgatgccgag
    34261 cgcatgattg cctcgctcaa gggtgccgcg ctgctcgccg gcgtgcgcgg ccgcccgccc
    34321 gccgacacgc gcgcgctgac cgaggtgctg ctcaggctgt ccgacctcgc gcgcgcctgt
    34381 ccggccattg ccgagctcga catcaacccg ttgctggtgc tgcccgaggg caagggcgcg
    34441 cttgccgccg acgcgctgat caccctcggc ggcgaggccg gtcgaaaggc cgactccaaa
    34501 tgaatgatta acaatcaaac gatcacggca ccccacgtca cgtcttgtca gtcccgcttt
    34561 cgagcgtgtg aaggaatcgg gagattgccc ggatttgtgg ccagcccgag cacggggggt
    34621 aaggatgagg agactttgag tttcggttcg cggctggatc atgggcgttg cctcgcgggc
    34681 gtgatcatgc tgcgacgagc gggcgcggca gccagcgccg ccagccgagc gcgatgccgc
    34741 ggcggatatc gtgggcgatg gcgtgcaaca gcgccgcgca tcggactttg tccagtccgc
    34801 gcggacggaa gcgatgcagg ccgtggttct tgaagtcggc attgacggtc tcgatccggc
    34861 ggcgccgccg gttgacggct ttgccgtcct cgccggccat gcgcgcccgc ctctccgtca
    34921 gggttgccgg ctccttgctc atcggcctcg ccagtccggc ctcgttgcgc cgtctcttgc
    34981 ggcaacgccg cgagcctggt ccgcaccgcc gcggcccggc tctcgacccc gcgcgccggc
    35041 cacctcgcgc gccgcccgcc cgggccggtc gcagcgggcg gtaccggcac cggccggtcc
    35101 tggtgtaagg tccatccgtg cccgcttggc ggaactggta gacgcacggg acttaaaatc
    35161 ctgaggccct cgggccgtgt cggttcgaat ccggcagcgg gcaccagcgc aggcgaccgg
    35221 tcggagacac tgccatcacc tgacttttgc accacaccgg cggggtgcct tgacgggtca
    35281 gtgcctgacc agggcacagg cggtcgattt cgaaaggaga gctcgccgcc gaaccgcctt
    35341 tctcgggatg ccgtgcaggc cgagcaggtg acggtgatcg cggacgccgg ctactccaac
    35401 ggcaagcggg ggtgacgatg aaggtggcgc gacgggccgt cttctttcca agatgaagcc
    35461 ccattttggg ccaggctcta ggcgggccag agggtatggc agcagcagtg gagatcgtcg
    35521 ccgggttccg ggcgctcgcg accagcacca tcggcaacgt cctcgacgag ctcggggtgg
    35581 gcggcctgat cgtcaatttg cggccgctgg tcgcgaagcg cccgttctgc ggcccggcgg
    35641 tcaccgcgca ggagactcac ggcccgttcg ggagctatgc gcccgaggac ttccgggtcg
    35701 gcgcgatcct cgatgccgcc ggcgcgggcg atgtgttggt gatcgccaac gacggcgcgc
    35761 aggtctcgac ctggggcggc ctcgcctcgc tcgccgccaa gcgcaagggc attgccggcg
    35821 cggtgatcga cggcggcgcg cgcgacgcgg acgagatcga ggcgtgcggc ttctcggtct
    35881 atgcccgcca cctggtggcg acgggcgggc gcaagcgcat ccgtgtcgac gcgatcggcg
    35941 tgcccgtcgt ggtcgacggc gtcgcggtca gccccggcga catcatcgtc gccgactcga
    36001 ccggcatcgc ctgcgtgccg gccgccgccg cggaacaggt cctgcgcctc gcgcgccagt
    36061 tcgacgccaa cgaccgcgcc tcggcgaagc tgctcgatca gggcctcagc ctcgccgacg
    36121 ccatgcgccg ctacgacacg atctagcgca cgccgccggc cgcgtacgcc ttcctgcccg
    36181 gatcacgtca agggaaagag gtcaggctcg gaaaacgggt tcgttcgacg gcaagaccgt
    36241 cgcaacctcc cgcgtcaggg ctgctggatc gactggatgt agtacacgag ctccagcacg
    36301 cgcgcccgca cccaaaattc gtttgattcg tcgggtccat attcggacac atactgccgc
    36361 atgtagcggt cgccccagac cggcatgtct cgatcgccat gcgcggccac ctcggcgcgc
    36421 ccgtcgataa cttgcaacac ccgatcgaac ggaaatacgc ccccgttgtt cttggcgagt
    36481 tgggtcagat cgccgggatc catgccttta aagagcctca tcagaaaacc cgcgcccttg
    36541 ccgtcgacgc cgtggcacga cgcgcaggag tttctgaact cgcctgcgct cggttcgagg
    36601 ttcatccgca cgtcctgcgc gctcgccgaa gccgtcggga tcaggatcag cgcggccgcc
    36661 gccgcaaaag cgcgatgtaa cgggctcatc atgtttgggc accttacgac atgctcatca
    36721 tcgttgcgct taaggctcct cgcaatcgca aggagtgtcc ttaatgtgtg ttaatcaggc
    36781 attccccaac tggctttccc ttggtgatgc aatggccggc acgcggggat agccagggct
    36841 ctcttgtctc cggttcgcgt cggatgccaa gtgccggcct ctgcggcaga ggatgtgctc
    36901 aacctcgcgc gtcggttcga cgccaatgat cgcacctcgg cgaagccgct cgatgagaag
    36961 ctcagcctcg ccgagcccat gcgccgcttc gacgcgatct agccccgctt ggtgcgggcg
    37021 aagaacttag ccatggcggc ttggggctcg cccgactcgt aagccgcgcg ctggaggggg
    37081 acagcggcct cgaacacctc gtcgaagccg gcctgggtca tctggcgcag gcgccgcttg
    37141 gtgagccgca gcgcattcgg cggcttggcg gcgagctcgc gcaccgcggc gcgcgcctca
    37201 tccagcacct ccgcggccgg caccagccgg gtcaacaggc cgagccgcaa ggcctcctcg
    37261 gcctcgacca ggcggccggt cagcgccaag tcgcgggtct gggcgtggcc caaagtgagg
    37321 ctcatcaggt gcacgccgat gacgcttgcc aggccggcgt tgatctcggg ctggcccatg
    37381 cgcaccccgg catggccgat gcgcaggtcg cacagcagcg cgagctggta gcccgcgccc
    37441 gcggcgacgc c
//
LOCUS       DASWHU010000043         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_383482, whole genome shotgun sequence.
ACCESSION   DASWHU010000043 DASWHU010000000
VERSION     DASWHU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383482"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGB88_01670"
     CDS             complement(<1..572)
                     /locus_tag="VGB88_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646175.1"
                     /translation="MSDGAAPALRLEAVVRSFVQGERRLEVLRGASLTVAGGEMVALV
                     GPSGSGKSTLLHIAGLLERPDAGEVEIAGRPCGRLGDRERTAVRRRALGFVYQFHHLL
                     PEFTALENVMMPQMVAGVPRARARARAAALLERVGLAERAGHRPGQLSGGEQQRVATA
                     RALANAPRLLLADEPTGNLDPETAGRVLGEL"
     gene            complement(565..1812)
                     /locus_tag="VGB88_01675"
     CDS             complement(565..1812)
                     /locus_tag="VGB88_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX9646176.1"
                     /translation="MFGAFERWVAWRYLRARRREGFISVVAGFSLVGIALGVATLIIV
                     LSVMNGFRAELLTRLLGINGHISVQAQQAGEGLADFDTLAASLRALPRVTRATPLIEA
                     QVMATANGKARGALVRGTRADDLRARELVAGSASEGALERFGNEDGVLVGYRLAGGLA
                     VEPGDSITLISPEGRSTVFGTVPRARAYPVLGTFNLGMSEYDGVLVFMPLAQAQVHFR
                     LPDMVTEIELVTTDANTAWLTAQEITGAFPDVYASAWQQRFQHLANALQVERNVMFLI
                     LTLIILIAAFNIIASLVMLVHDKGKGIAILRTMGATRGAVLRIFFLTGAAIGVVGTVA
                     GLVLGLLFTDNIQTLQGWLDALLGTELFSAEIYYLSHLPAKIDPGEVTTVVIMALALS
                     FLATVYPAWRAARLDPVEALRYE"
     gene            complement(1815..3149)
                     /locus_tag="VGB88_01680"
     CDS             complement(1815..3149)
                     /locus_tag="VGB88_01680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9646177.1"
                     /translation="MRHGYSARLTQYFLPTLKETPAEAQIVSHRLMLRAGMIRQSSAG
                     IYSWLPLGLRVLRKIEQVVRREQDRIGHEMLMPTIQPADLWRESNRYDAYGPEMLRVS
                     DRHQRDMLYGPTNEEQITDIFRGSVKSYRDLPKMLYHIQWKFRDEVRPRFGVMRGREF
                     LMKDAYSFDLDLAGARHAYNRQFVAYLRTFAQMGLKAIPMRAETGPIGGDMSHEFVIL
                     ADTGESEVHCDAALLDFDLPGEEAYDRADLSALVERWTGVYAATDDKHDPAEFAARVP
                     AERRRAARGIEVGHIFYFGTKYSEAMGAQVTGPDGEAVTVEMGSYGIGVSRLVGAIIE
                     ASHDQRGIIWPQSVAPFGIGLVNLKSGDAACDAACADLFQRLARAGCDVLYDDRDERA
                     GVKFADMELIGLPWQLRVGPRGLKDGVVELHVRATGKTETMTPDAAVTRLAG"
     gene            complement(3221..3655)
                     /locus_tag="VGB88_01685"
     CDS             complement(3221..3655)
                     /locus_tag="VGB88_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646178.1"
                     /translation="MAALLGAAFCPIAGATAARAEDRAGTVIIIAPADCAALTAHVPD
                     ESVDYVPGVDVQGRPVAPADLPGHEPLSLDAAEIGIDPRIPLTEYYDPPASLAPVIGE
                     AEVEIGRITVQGGVAYLGRQALGDPQQAAIARACAERLRRRP"
     gene            complement(3917..4201)
                     /locus_tag="VGB88_01690"
     CDS             complement(3917..4201)
                     /locus_tag="VGB88_01690"
                     /inference="COORDINATES: protein motif:HMM:NF018978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX9646179.1"
                     /translation="MSGVEVAVVFALLWWVVLFTTLPFGVRRTEQPDPGTEVGAPEHA
                     HIGRKLLATTLITGAITAAIYVAAEAGLLPVRDWLAPERPLAGRTGEISK"
BASE COUNT          648 a         1500 c         1521 g          598 t
ORIGIN      
        1 agctcgccga gcacccgccc cgcggtctcc gggtcgaggt tcccggtcgg ctcgtcggca
       61 agcagcagcc gcggggcgtt ggcgagcgcg cgggcggtgg ccacccgctg ctgctcgccg
      121 ccggacagct ggccgggccg atggccggcg cgctcggcga gcccgacccg ctcgagcagg
      181 gcggcggcgc gggcccgcgc gcgggcccgg ggcacgcccg cgaccatctg cggcatcatc
      241 acgttctcca gcgccgtgaa ctcgggcagc aggtgatgga actggtagac gaagccgagc
      301 gcgcgccggc gcaccgcggt gcgctcgcgg tcgccgagcc gcccgcacgg gcgtcccgcg
      361 atctcgacct cgccggcgtc gggccgctcg agcagcccgg cgatgtgcag caaggtcgac
      421 ttgcccgatc ccgacggccc caccagcgct accatctcgc cgccggcgac ggtgagcgag
      481 gcgccgcgca gcacctcgag ccggcgctcg ccctggacga agctgcgcac gaccgcctcg
      541 agacggagcg cgggcgcagc cccgtcactc atagcgcagc gcctcgaccg gatcgagccg
      601 cgccgcccgc caggcgggat agacggtggc gaggaacgac agcgccagcg ccatgatcac
      661 cacggtggtg acctcgccgg ggtcgatctt ggccggcaga tgcgacaggt aatagatctc
      721 ggccgagaac agctcggtcc cgagcagcgc gtcgagccag ccctgaaggg tctggatgtt
      781 gtcggtgaac agcagcccca gcaccagccc ggcgacggtg ccgaccacgc cgatcgcggc
      841 gccggtgaga aagaagatgc gcagcaccgc gcccctggtc gcgcccatgg tgcgcaggat
      901 cgcgataccc ttgcccttgt cgtggaccag catcaccagg ctggcgatga tattgaacgc
      961 ggcgatcagg atgatcaggg tgaggatcag gaacatgacg ttgcgctcca cctggagcgc
     1021 attggcgagg tgctggaagc gctgctgcca ggcgctggcg tagacgtcgg gaaacgcgcc
     1081 cgtgatctcc tgcgcggtga gccacgctgt attggcatcg gtggtgacca gctcgatctc
     1141 ggtcaccatg tcgggcaggc ggaagtggac ctgggcctgg gcgagcggca tgaacaccag
     1201 cacaccgtcg tactccgaca tgccgaggtt gaaggtgccg agcaccggat aggcgcgggc
     1261 gcgcggcacg gtcccgaaca cggtcgagcg gccctcgggc gagatcaggg tgatgctgtc
     1321 gcccggctcg acggcgagcc cgccggccag ccggtagccg accagcaccc cgtcctcgtt
     1381 gccgaagcgc tcgagcgcgc cctcggaggc gctgccagcc accagctcgc gcgcgcgcag
     1441 gtcgtccgcg cgggtgccgc gcaccagcgc gccgcgcgcc ttgccgttgg cggtggccat
     1501 gacctgggcc tcgatcagcg gcgtcgcgcg ggtgacccgc ggcagtgcgc gcagcgaggc
     1561 ggcgagcgtg tcgaagtcgg cgagtccctc gcccgcctgc tgggcctgga ccgagatgtg
     1621 gccgttgatg ccgagcaagc gggtgagcag ctcggcccgg aagccgttca tcaccgacag
     1681 cacgatgatc agcgtcgcga ccccgagcgc gatgccgacc agcgagaagc cggcgaccac
     1741 cgagatgaag ccctcgcggc ggcgcgcccg caggtagcgc caggcgaccc agcgttcgaa
     1801 cgcgccgaac acgctcagcc cgcgagccgg gtgaccgccg cgtccggcgt catggtctcg
     1861 gtcttgccgg tggcgcgcac atgcaactcg accacgccgt ccttgagccc gcgcgggccg
     1921 acccgcagct gccacggcag gccgatcagc tccatgtcgg cgaacttgac cccggcgcgc
     1981 tcgtcgcggt cgtcgtagag cacgtcgcag ccggcgcgcg cgagccgctg gaacaggtcg
     2041 gcgcaggcgg cgtcacaggc ggcatcgccg ctcttgaggt tgaccaggcc gatgccgaac
     2101 ggcgccacgc tttgcggcca gatgatgccg cgctggtcgt ggctcgcctc gatgatcgcg
     2161 ccgaccaggc gcgagacgcc gatgccgtag gagcccatct cgaccgtaac cgcctcgccg
     2221 tccgggccgg tcacctgggc gcccatggcc tctgagtact tggtgccgaa gtagaagatg
     2281 tggccgacct cgatgccgcg cgcggcgcgc cggcgctccg ccggcacgcg ggccgcgaac
     2341 tccgcgggat cgtgcttgtc gtcggtcgcc gcgtagaccc cggtccagcg ctcgaccaga
     2401 gccgagaggt cggcccggtc gtaggcctcc tcgccgggca ggtcgaaatc gagcagcgcc
     2461 gcgtcgcaat ggacctcgct ctcgccggtg tccgccagga tgacgaactc gtggctcatg
     2521 tcgccgccga tcgggccggt ctcggcgcgc atcgggatcg ccttcagccc catctgcgca
     2581 aaggtgcgca agtacgccac gaactgtcgg ttgtaggcgt gccgggcgcc ggcgaggtcg
     2641 aggtcgaagg agtaggcgtc cttcatcagg aactcgcgcc cgcgcatcac gccgaagcgc
     2701 ggccgcacct cgtcgcggaa cttccactgg atgtggtaga gcatcttcgg caggtcccga
     2761 tagctcttga ccgagccgcg gaagatgtcg gtgatctgct cctcgttggt cgggccgtaa
     2821 agcatgtcgc gctggtggcg gtcgctgacg cgcagcatct ccggcccgta ggcgtcgtag
     2881 cggttgctct cgcgccacag gtccgccggc tgaatggtcg gcatcagcat ctcgtggccg
     2941 atcctgtcct gctcccgacg caccacctgc tcgatcttcc gcagcacccg caagccgagc
     3001 ggcagccagc tgtagatgcc ggcgctcgac tggcggatca tgccagcgcg cagcatcagc
     3061 cggtgcgaga cgatctgggc ctcggccgga gtctccttca gggtgggaag gaaatactgg
     3121 gtaagacgcg cggaatagcc gtgtctcatc tggcctggcg ccggtccggt tgacggtgcg
     3181 gcaatctatg aaatggccgg ggtctaggca acgccgtcgg tcacgggcgc cggcgcaggc
     3241 gctcggcgca ggcgcgcgcg atcgccgcct gctgcgggtc gccgagcgcc tggcggccga
     3301 gataggcgac gccgccctgc accgtgatcc ggccgatctc aacctccgcc tcgccgatca
     3361 ccggcgcgag cgatgccggg ggatcgtaat actcggtcag cgggatgcgc gggtcgatgc
     3421 cgatctccgc cgcatcgagg ctgagcggct cgtgcccggg gaggtccgcc ggcgcgaccg
     3481 gccggccctg gacatcgacc cccggcacgt agtcgaccga ctcgtccggg acatgggcgg
     3541 tgagcgcggc gcagtccgcg ggcgcgatga tgatgaccgt gccggcgcga tcctcggccc
     3601 gcgcggcggt ggcgccggcg atcgggcaga acgccgcccc gagcagcgct gccacaacgg
     3661 ccggttgaat cctgcggtgg acccaaacgg acagttcaag gctttgtcgc ataatgtttt
     3721 ttcgtgaccc ctcctggccg tgaggctata gtctactgat ctggacggga cggcgcgagg
     3781 gagccgttcg gcccaggttt ggggaggcaa cgataacaag gactcgggcg cgatccgtcg
     3841 catgccggcg gctcgcggtc aacggtcagg gaacgacgac tatcgaggag caggacttac
     3901 ggtcttgctt ctttttttac ttcgagatct cgcccgtccg gccagccagc gggcgctccg
     3961 gcgccagcca gtcgcgcacc ggcagcaagc ccgcctcggc cgcgacgtag atcgcggcgg
     4021 tgatcgcgcc ggtgatcagg gtcgtcgcca gcagcttgcg cccgatgtgc gcgtgctcgg
     4081 gcgcgccgac ctcggtcccc ggatcgggct gctcggtccg ccgcaccccg aacggcaagg
     4141 tggtgaacag caccacccac cacaggagcg cgaacacgac cgcgacctcg acccccgaca
     4201 tggccggggc tcacgcctgc tcgatctcga ccagggtccc gcacaggtcc ttcgggtgca
     4261 ggaacag
//
LOCUS       DASWHU010000044         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_385517, whole genome shotgun sequence.
ACCESSION   DASWHU010000044 DASWHU010000000
VERSION     DASWHU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385517"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGB88_01695"
     CDS             <1..257
                     /locus_tag="VGB88_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9646180.1"
                     /translation="IAGRDAADWAGVFAGEDCCCSIVADLRAALDDAHFRARGLFDHL
                     LESAHGERIAALPVPVAPEFRAAPGTPQRAPALPDDDGAR"
     gene            254..1249
                     /locus_tag="VGB88_01700"
     CDS             254..1249
                     /locus_tag="VGB88_01700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX9646181.1"
                     /translation="MRAVVVERFAPLAAARMGELPEPQPGPGEVLIRVHAAEANYPDI
                     LVIEGAYQVKPPLPFAPGKAAAGVVEATGAGVDAFAAGDRVAAQVEYGAYAEKLVAPA
                     ASCFRMPDAMPFVDGAALGLVYQTAHFALRERASFARGEVVLVLGASGGIGTAAVQLA
                     KAFGARLVIGGVLGADNAAVARAAGADLTVDLGAGDLRDSLRAAVHDATDGHGADIVI
                     DPVGGAANAAALRALAWRGRMVIIGFASGEIPTIKANYLLVKNIAVSGLQWSDYRDRD
                     PAWVRRAQDEIYALYLDGKVKPRIGGTFPLARFAEALELLRAGKAQGKLVLTLDG"
BASE COUNT          211 a          548 c          547 g          211 t
ORIGIN      
        1 tcattgccgg gcgcgacgcc gcggactggg ccggggtgtt cgccggcgag gactgctgct
       61 gctcgatcgt cgccgacctg cgcgccgcgc tcgacgatgc gcatttccgc gcccgcggcc
      121 tgttcgacca cctgctcgag agcgcacatg gcgagcgcat cgccgccttg ccggtgccgg
      181 tggcgcccga gttccgtgcc gcgcccggca cgccgcaacg cgcgcccgcc ttgcccgacg
      241 acgacggcgc gcgatgaggg cggtggtcgt cgagcgcttc gcgccgctcg ccgcggcgcg
      301 catgggcgaa ctgcccgagc cgcaacccgg accgggcgag gtgctgatcc gggtccatgc
      361 cgccgaggcc aactatcccg acatcctggt catcgagggc gcctatcagg tcaagccgcc
      421 gctgccgttc gcgcccggca aggcggcggc cggggtggtc gaggcgacgg gcgcaggggt
      481 cgatgcgttc gcggccggcg accgggtcgc agcgcaggtc gagtacgggg catatgccga
      541 gaagctggtg gcgcccgcgg cgagctgctt ccgcatgccc gatgcgatgc cgttcgtcga
      601 cggcgcggcg ctgggcctcg tctaccagac cgcgcacttc gcgctcaggg agcgggcgag
      661 cttcgcccgc ggcgaggtgg tgctggtgct gggcgcctcg ggcggcatcg gcaccgcggc
      721 ggtccagctc gccaaggcgt tcggcgcgcg cctggtgatc ggcggcgtgc tcggcgcgga
      781 caacgccgcg gtcgcgcgcg cggccggcgc cgacctaacc gtcgatctcg gcgccggcga
      841 ccttcgggac tccttgcgcg cggcggtgca tgacgcgacc gacggccatg gcgccgacat
      901 cgtcatcgac ccggtcggcg gcgcggccaa tgcggcggcg ctgcgcgcgc tcgcctggcg
      961 cgggcggatg gtgattatcg gcttcgcctc gggcgagatc ccgaccatca aggccaacta
     1021 tttgctggtc aagaacatcg ccgtctcggg cctgcaatgg tcggactacc gcgaccgcga
     1081 cccggcctgg gtgcgccgcg cccaggacga gatctacgcg ctctacctcg acggcaaggt
     1141 caagccccgg atcggcggca ccttcccgct cgcgcgcttc gccgaggcgc tggagctgct
     1201 gcgcgccggc aaggcgcagg gaaaactcgt cctcacgctc gacggctgag cgccggcgcg
     1261 tgctccggga acaggccctc gaggccggcg cgcgaggcgg cgcacacgcc gcgctcggtg
     1321 atcagcccgc tcaccaggcg cgctgttaat cgtcgactgc aaccacagcc cgcgcatcga
     1381 cgtcatcgac gttaacggaa aggatggccc ggccaattac ggtgatacgc gacgacagcc
     1441 attacaaaga gtatcgatac tacaactaac gccccggacg ttgcccatcc gcccggcagc
     1501 aaatcagagg tcgcgtt
//
LOCUS       DASWHU010000045        26536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3341633, whole genome shotgun sequence.
ACCESSION   DASWHU010000045 DASWHU010000000
VERSION     DASWHU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 26536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26536
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341633"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VGB88_01705"
     CDS             <1..160
                     /locus_tag="VGB88_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX9646182.1"
                     /translation="APARVRALGSGRAEVVFESPQKAIAPGQAAVVYQGSRLMGGGWI
                     ERPAAPAA"
     gene            231..307
                     /locus_tag="VGB88_01710"
     tRNA            231..307
                     /locus_tag="VGB88_01710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:265..267,aa:Met,seq:cat)
     gene            complement(363..1163)
                     /gene="soxA"
                     /locus_tag="VGB88_01715"
     CDS             complement(363..1163)
                     /gene="soxA"
                     /locus_tag="VGB88_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX9646183.1"
                     /translation="MRRPAGIAAAVVAVAATLARAVAADAPTPPIAPDELRSGYHFQS
                     PATRAMQDDEFDNPGLLWVGRGESLWREAAGATGQACADCHGDPAAGMAGVAARYPAV
                     DAGTGELLNLEARINRCRAERQGAPALAYDSAELLALSALVAYQSRGLPIRVAIDGPA
                     RPFFEAGRSAYTTRQGQLNLACSHCHDDHWGERLRGELLSQGHPTGYPIYRLEWQGMG
                     SLHRRLRSCDIGVRAQPGPLGAPAYVALELYLAWRAQGLAIETPAVRR"
     gene            complement(1160..1480)
                     /gene="soxZ"
                     /locus_tag="VGB88_01720"
     CDS             complement(1160..1480)
                     /gene="soxZ"
                     /locus_tag="VGB88_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX9646184.1"
                     /translation="MANTRLAVPAQAKKGEVIEIRTMIQHPMETGHRPDSVGRAVPRD
                     IIRSFVCTYNGVEVFRAELFPAIAANPLLKFFTVATESGELAFAWTDDHDVTHTETAA
                     ITVT"
     gene            complement(1491..1979)
                     /locus_tag="VGB88_01725"
     CDS             complement(1491..1979)
                     /locus_tag="VGB88_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HEX9646185.1"
                     /translation="MTARPARTRPRPAGATRRQVLAGAAGLAAVALLPGAAGGAPESM
                     AAAIAEVAGDSPVTPGGMVLEVPQLSENGNAVPLTVTVDSPMTDADHVASIHVFTEHN
                     PLPNVAHFYLGARAGRAQVSTRIRLAGSQRVVALARLSDGSLRSAEVEVVVALAACIE
                     PA"
     gene            complement(1976..2434)
                     /gene="soxX"
                     /locus_tag="VGB88_01730"
     CDS             complement(1976..2434)
                     /gene="soxX"
                     /locus_tag="VGB88_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX9646186.1"
                     /translation="MLAGLGAALGGGPAGSAESVAAYTVAEDEIPRPLTAAPGDPARG
                     EDIVRDRELGNCLICHALPIADEPFQGTVGPDLAGLAGRLTAGQMRLRVVDATLVNAD
                     SAMPAYHRIDGLERVADEYRGKAVLSAAQVEDVVAYLLTLRAPRAGEDRQ"
     gene            complement(2470..4641)
                     /locus_tag="VGB88_01735"
     CDS             complement(2470..4641)
                     /locus_tag="VGB88_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9646187.1"
                     /translation="MAVAPELSRRSFIVGSAAAVGGLSLGFHVPLPGKAADAMADEVN
                     AWVVVKPDDTVVIRIARSEMGQGTLTGLAQLVAEELECDWSKVTTEYPTPGQNLARDR
                     VWGNFSTGGSRGIRESHDYVRRGGAAARQMLIQAAAAEWQVPASECSAANSVITHGPS
                     GRTTTFGKVARAAAAIPPPTEIALKDPMQWKIAGKSVKRLDTAPKLTGAQVYGIDLKQ
                     PGMLNAAIKDCPVFGGWLRSFDAAKVADMPGVRRVVRVGESAVAVVADTWWHAKTALD
                     ALPVVWDEGENLGVSSATIAAILEQALDAEEAFVGHEVGDARAALAGAAKTVAAVYSY
                     PFQNHATMEPMNATALFTGDRCEVWTPTQNGEAALAAATAASGLPIEKCDVHKIHLGG
                     GFGRRGAFHDYVHQAVAIAKEMPGTPIKLIWSREEDMLHGQYHPVTQAKLIGGFDTDG
                     NLTALHMRISGQSILAAVFPQRLEDGRDPVVFQGLMPSGDGTFGYTIPNLLIDHAMRN
                     THVPPGFWRGVNHNQNAIYLECFMDELAHAAGQDPLAFRRKLMANHPKHLAVLEAVAD
                     KAGWGTPAPAGVYRGLCQHMGFASYTASCAEVSIAADGALRIHRIVAATDPGHAVNPD
                     QIAAQVEGSFVYGLSAMLYGECTVKDGRMEQDNFDSYPVLRMAEMPKVETIVMPSGGF
                     WGGVGEPTISVAAPAVLNAIFAASGKRVRSFPLRHHDLAAV"
     gene            complement(4698..5147)
                     /locus_tag="VGB88_01740"
     CDS             complement(4698..5147)
                     /locus_tag="VGB88_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9646188.1"
                     /translation="MVNLNLNGQARAVDVDPETPLLWVLREQLGLTGTKYGCGIGQCG
                     ACTVHVDGQATLSCLTPVAAVENAAITTIEGLSPDASHPVQKAWLALDVPQCGYCQSG
                     MIMAAAALLWRAPAPTDADIDAGITNICRCGTYNRVRAAIHLAAAGG"
     gene            5515..6369
                     /locus_tag="VGB88_01745"
     CDS             5515..6369
                     /locus_tag="VGB88_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX9646189.1"
                     /translation="MASLRFGGMGWERDIPDFRDYSADSADVKSVLAKSKPLKAAAKA
                     APAKVDLREWCPPIEDQGNIGSCTANAGVGMLEYFERRAFGKHLDGSRLFLYKATRNL
                     LGWEGDQGAYLRDTMKAMCLFGVPPESYWPYRTQRFDDEPTPFCYAFAQSYKAMQYYR
                     LDPAGSPAAQVLADVKTYLAAKLPAMFGFTVYSSMPGIGAGTGDIPFPSSGDRREGGH
                     AVLAVGYDDNRRIASEKGALLIRNSWGEEWGDKGYGWIPYTYVQRGLAVDFWSLVRAE
                     FVDTDLFK"
     gene            complement(6377..7483)
                     /locus_tag="VGB88_01750"
     CDS             complement(6377..7483)
                     /locus_tag="VGB88_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9646190.1"
                     /translation="MHREIALYTLDGVKDARITTHYVPTADKLSISMLRFWKADCDDV
                     VVVIHGLTTSSDMFIMPEHQNLVSWLHTQGFGDVFTLDFRMSNRHVYNLAPHRYSMDD
                     CALYDHPAAIEAVRRISGARRIHVICHCLGSMSFVMSLFAKQVDGIASVIANSVALTP
                     RVPAWSRIKGAMFPFLLENVLSVPYVSPRWADDPTLTFGKALNWAVSRFHRECDVPAC
                     HMLSLMWGTGWPALYEHDNLLPVTHARGGDLYGGTSLNYHRHVLKMVRAGNTAVRMYP
                     DKDAHAALPDNYLAHAREIGTPVLFMTGRNNRVFTDSNILCFNRLMELAPGNRHELLI
                     LDGYGHQDPFMGKDAAGDVFPRFLPFLAKHAGRA"
     gene            complement(7491..9080)
                     /locus_tag="VGB88_01755"
     CDS             complement(7491..9080)
                     /locus_tag="VGB88_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9646191.1"
                     /translation="MVRRYDAIVVGSGFGGAPVACRLAEAGRKVLVLERGREWRAEDY
                     PRAPGDAWLWDDDHPERHNGWIDARFFGHMTVIQGAGVGGGSLIYANVSVEAEPWAFE
                     RGWPPGISYESLAPHYAEVGRMLDVHELPDGQLTERTKLMRDGAEALGHGDRVRKLPL
                     AVTFSDDWHYGLEDAHDAKWSKRWTNAHGQVQGTCIHCGNCDIGCRVGAKNTLDLNYL
                     AAARASGAELRPLHVVRSIAPLDDGYSVGVDRIEDGRLIADDCAARIVVLAAGSLGST
                     ELLLRCRDDYRTLPNVSRRLGLGWSSNGDFLTPAVYRDRRVSPTRGPTITAAIDFLDG
                     SRDGRRFFVEDGGFPDAIGNTIGELAQSGRRLGGVLGKAFARLAKEARGGDPLDCVMP
                     WFGQAADASNGRLYLGRRWWWPFGPRKILLDWDVADSEATIQAMVDMHKALSRATGGV
                     PIEPLYWRWAKDLITPHPLGGCALGSGPADGVVGAGGEVFGHPGLYVADGAILPKAIG
                     LNPSRTIAALAEHVAQGILSG"
     gene            complement(9087..9710)
                     /locus_tag="VGB88_01760"
     CDS             complement(9087..9710)
                     /locus_tag="VGB88_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646192.1"
                     /translation="MRGITFSETMAGPFALDEQDPERGRATGARRGTRLTMNARVAIA
                     DLDAFVADPDHAGELAGSVDFAPLGVALTAHDGVFNLFSPSGQPELKLMVYELAISAA
                     GKPYYVAGRKEVRDDRGFDLWSDTTTLYTTLHQGAGKDGPVVGAGILGLGVRDLGDLL
                     ATVRVTGAGSPLEIAETLTKFGVFFMGELWHSYGPEMLKSFTGRGRR"
     gene            complement(9727..11475)
                     /locus_tag="VGB88_01765"
     CDS             complement(9727..11475)
                     /locus_tag="VGB88_01765"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX9646193.1"
                     /translation="MSILRFPGLGPIVGHTTPTSCRIWIRAGDPEDQGAQLASARRTI
                     GVIALCERNGKPIAKPDVYYFRLHREFDRTGTLNLGDEVGIVPDWDPHTRRGRSKGPA
                     TLAPATRHAAVVGTLVIDDPFADDETVTDDYLAHRLPDPDNLRSDLETLLKRDRSRCV
                     AEFFTFPKAEAAAADELSFILGSCRYPGLLWKAKEADTIFGPLRAEAAGEVGDPKRRR
                     AEFALMVGDQIYADMLNRHVPLGLADTFEEFQQRYLTAFGSPNMSRLLRGMPTYMILD
                     DHEIEDNWTQDRLSKAAARRVFNIAIGAYMSYQWSHGPRSFGRRLYCTFDCGGYPFFV
                     LDTRTQRFIDDVEDSLDDNHMLGRPSMGGEEPSQLERLLRWLVERQQQGGNLPKFVVS
                     SSVFVPSPMSARTGRLDAPHLHEARHQMIQWMQASDSWPAFPVTRRAILRCLIDNAVQ
                     NVVFLSGDIHCSNVARMRFAGAPTAEALRAFSITSSAFYWPFFFADGDPSNYVHDSTQ
                     ADQRDTFLISEAETMEYQAWNFTQDDNYCRIDVDRASASITVSPFNTEGELIRKRDWL
                     NRPTDRLIAKLELAPW"
     gene            11966..12280
                     /locus_tag="VGB88_01770"
     CDS             11966..12280
                     /locus_tag="VGB88_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646194.1"
                     /translation="MANIDAVRWIEVTDRSTPVGYREFLDPVSGSRCVIRAHTVDEDG
                     RPTGAAHGNDPAGGVGPGLDQASGANGARFFIYSVVATGLALAGIAITVWQALKMFGG
                     DS"
     gene            complement(12313..12885)
                     /locus_tag="VGB88_01775"
     CDS             complement(12313..12885)
                     /locus_tag="VGB88_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646195.1"
                     /translation="MAALAAVLLLVTGSAGADPARTPTDFVSDLGDRLLTELKRDLPR
                     GELQGRLQELFTEAFDVPVVAKFVLGRHWKAATESQQHEYLGLFGRYVGGIYATQFSD
                     YSGETFEIRAQTQLSGGDVLVRLDIIRPDGPPLPVDFRVRREQETLRIVDVAVEGLSL
                     IVTKRDEFGSLVQREGMEGLLEALRRTSAG"
     gene            complement(13106..16183)
                     /locus_tag="VGB88_01780"
     CDS             complement(13106..16183)
                     /locus_tag="VGB88_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9646196.1"
                     /translation="MKISELCIRRPVLATVMSVVLTLVGIVSYQRLNVREYPDIDAPV
                     VNVQTSYRGADAKIVESQVTKVLEDSLAGIEGIDYMTSISRQEQSQITLTFGLDREPD
                     SAAADVRDRVGRVRAQLPDDIDEPVIQKVEADAQAMMYLAFTSDRHSPPQITDYADRF
                     VKDRLQILPGVAEVSIFGERRYSMRIWLDPGRLAGLGLTPVDVEDALRSQNVEIPAGR
                     IESVDREFSVLSETDLQTPAEFDDLVLKRSGGYLVRLADVGHAEIGAQEERRVVRYNG
                     ETAIALGVIKQATANPLEVSNAIYGALPSIRETLPEGMKVAVAYDTTIFINASIDNVY
                     ATIGEAVVLVVLIIFLFLRSVRATLVPIVTIPISLIGACALMALFGFTINTLTLLSMV
                     LAIGLVVDDAIVMLENIYRHVERGQPPLRAALDGSREIGFAIVAMTLTLVAVYAPIGF
                     MTGNTGRLFTEFAWALAGAVAVSGFVALTLSPMMCSLLLKPHGVRHGLLFRLGEAALN
                     GLTAGYRATLRLGLRVRPLILLIGLGVAGAGVPAYMSLTSELAPYEDQGIIRLFYLAP
                     EGATIGYTDRYARRFEPIVESIPEVAHYFVVSGYPVVSQGITFLNLKPWDERERSAGE
                     IAAEVGPKASQIPGVIAFPILPPPLGQARHSKPVEIVIGTIRSYDELAGWVDQVMARA
                     EDYPGLANIDTDLKLNTPQLRVDVKRDKVASLGIDVAQLGRTVETLLGGRQVTRYKQD
                     GEQYDVIVQVADVDRTNPDDLHRIYVKSDGGQLVQLSNLVSIEETVAPKELNHFNQLR
                     AAKVTANLAPGTSLAEGLGFLETAAREIVPPDTQIDYSGQSREFKRSSADIYLTFALA
                     LAFIYLVLAAQFESFVDPFVIMLTVPLSMTGALVALHLTGGTLNIYSQVGLVTLIGLI
                     TKHGILIVEFANQIRARGRETREAVLEAATLRFRPILMTTGAMVLGAVPLALAEGAGA
                     QSRQDIGWVIVGGLLVGTFFTLFVIPCVYTYVTGKRSDAVAELPVLRQPSAAE"
     gene            complement(16180..17244)
                     /locus_tag="VGB88_01785"
     CDS             complement(16180..17244)
                     /locus_tag="VGB88_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9646197.1"
                     /translation="MAAAAACLAVLLSGAPGARAQDAKPAVAVESAIIAPTRLVESIS
                     AVGSLLSNESVVVSPEIDGRLTEIGFDEGRPVATGQMLIRLDSSIFRAELAQAEARQE
                     LSRHTYDRAQALFEKGHGSGQTLDESLAQMRADEAAAALARARLEKSTITAPFAGLVG
                     LRGVSVGDYVRAGQAIVNLESVDPLKLDFALPERYLRVIRVGQAVEVDLDAFPDESYV
                     GTVYALDPLIEPGGRSVSVRATLPNPQGHLRPGLFARVRLIVDEREQALVVPEEALIP
                     RGDRRYVYRIIDGVAVLTEVRLGLRQTGRVEVVDGLEAGDEIVVAGHAKIRDGAKVRV
                     IETRGAPDQEPAPGVVDASS"
     gene            17401..18033
                     /locus_tag="VGB88_01790"
     CDS             17401..18033
                     /locus_tag="VGB88_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9646198.1"
                     /translation="MSLALSAPRELSRSDQKKAAIIEAATQVFLRDGYGRANMDSIAQ
                     VAGVSKQTIYHHFGNKSDLFGAIIRAKCDRLLEPLEMPTESAMDLEGALASVARQFID
                     IMLAPSSLALHRVLMGEVHRFPELGRMSYQIGPARIVATIAAYLSRQGAAGRLDLPDA
                     KFAAEQFYAMLTGHLQLRALLGVEADPSPGQIERTIRHAVDTTLKIYAPH"
     gene            18166..18471
                     /locus_tag="VGB88_01795"
     CDS             18166..18471
                     /locus_tag="VGB88_01795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9646199.1"
                     /translation="MTRFVVLAQFRVKPGKRDSFVALAETDATAAVANEPGCRRFDVL
                     VPHDSTERVVLYEIYDDKAAFDAHLKSPHLAAFRKGIADLIEDRRIETFEVHEHADP"
     gene            complement(18518..18859)
                     /locus_tag="VGB88_01800"
     CDS             complement(18518..18859)
                     /locus_tag="VGB88_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646200.1"
                     /translation="MRVWFGMAAAALLAVAGALGAAAKDTIHVRLNNPTDMEWKVEVR
                     DMVCDNAVVWQGRMKPGEAAKVTVCADGNGAARLATAVIAGCASAKRAVYQDVADGAT
                     IDVAVAEDEDE"
     gene            complement(18938..20428)
                     /locus_tag="VGB88_01805"
     CDS             complement(18938..20428)
                     /locus_tag="VGB88_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646201.1"
                     /translation="MAVARRSRRSLRLALAGVLAAGPAQAHGFGQRYDLPLPLSYWLA
                     AAALVVGLSFALAAVAARRRAGPGRRRPWPRLPVGAAAKTMVPLLRALGLAVLVLVVA
                     AGLAGVQSPIRNIAPTLVWVLWWVGLVFVCVLVANPWPALNPWATLYDLGAWAWTRLA
                     RRPPPAPPLRYPRALGAWPAVALFAGFAWIELIWKHGEVPAALAAVILAYSVLTLAAM
                     VAFGRDAWLGGGEAFTLAFSVFGRFAPIAPAPGGGAELELRPYGAGLLVDAPASRSMV
                     CFVVLMLSTVTFDGLMETPLWSATVDAAFSCETLAPALFVMRDVFGNAAKGIETVALV
                     LFPIAVLAFFLVFVWIMARLAPLAAAPGGPAHYDLWALAGRFAFTLVPIAVGYHFAHY
                     LSFLAIAGQLAIPLASDPFGFGWDLLGTARYRIDISLINARVVWYVAVTAIVTGHVIG
                     VVLAHVAALRLFASRAAAILSQVPMLVLMVCYTMLSLWILAQPIVQ"
     gene            complement(20434..20844)
                     /locus_tag="VGB88_01810"
     CDS             complement(20434..20844)
                     /locus_tag="VGB88_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646202.1"
                     /translation="MTLPLSKWAGRRRDRRLAGAYLVAGLLLICVAGSTRAEGATVTR
                     DVTIADRAVVGDQVVRVTEGEHVVLRWTGDEPAEIHLHGYDLPARLVPGEVTAIEFDA
                     NATGRFPVTVHGFGDDHGEEHDESALLYVEVHPR"
     gene            complement(20848..22050)
                     /locus_tag="VGB88_01815"
     CDS             complement(20848..22050)
                     /locus_tag="VGB88_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9646203.1"
                     /translation="MFKLREALAVVTVALAAAPAAARADMLAIANYETKAEESLQSLQ
                     LQSATPVRREGIAIIELDRASPDYGKILIDMPMPPDTVLHHVFYNRDLTKAYVTALGK
                     EVLHVIDLGEFPYRARPIATPGCKVQEDIIFSDDNSRWFVTCMGSSNVAVGDAVKDEM
                     TGLIELPGAYPHGIALDESIDRLLVVNCIKPDMSAVGTTVEVIRASTGEHLASLPASD
                     KGSAAPVEIVFVPDSDPPVAYFTNMMAHTLAAAVWNPETESFDIRELFDFSTEGAAMP
                     LEMYFNKAVDRLYVTTADPGMFHIFDISAGPLAPKLLKTLPAAGGAHHVALTQDEKFA
                     FVQNSLLNLPGMSDGSITVVDLERQQVVDSIDAFKSQNLNPNSLTLLPAWYHPMGHFN
                     NGPGPGSM"
     gene            22291..24954
                     /locus_tag="VGB88_01820"
     CDS             22291..24954
                     /locus_tag="VGB88_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX9646204.1"
                     /translation="MAAAPARLMNARAPAAAIADAHAVPAAGVLSRLGVDPARGLGWR
                     EVAARRRRYGRNRLPAQPARSLWSILADQFKSLVVVLLAAAAALSWTLREWTEGTAIM
                     VVLLINGAIGFVTELRAVRSMEALRRLATVATRVRRAGRVRMRPAQALVPGDVVVIEG
                     GDIVTADLRLVEAARLQCDESTLTGESVPVDKSTEALPPEAPVTERANMLYRGTAVTR
                     GSGIGVVVATGAATELGQISALAQAAAPETSPLEKRLDRLGGQLVWATVALVAVVGGL
                     GVLRGNEPLLMIETAIALAVAAVPEGLPIVATMALARGMWRMARRNVLIRRLSAVETL
                     GATTVVLTDKTGTLTENRMTVTWLELDRLAVEVGGAGEAAFTAGGAALDARADPRAAM
                     ALRIAVACNNAALEPVGEDGAARGVGDPMEVALLLAAAKAGLDRATVLRDWPEQREEA
                     FSSDVKMMATVHRAGAGYMVAVKGAPEAVLAAATAVLGADGPIALDAARRAHWTRRID
                     RLAADGLRVLALAMKETADADDSPYRDLTLVGLVALIDPPRGEVPAAVAACRRAGVQV
                     VMVTGDHAETARAIARAVGIGDPGDATGGGGVFARVTPETKLDLVARYQAQGEIVAMT
                     GDGVNDAPALRKADIGVAMGRRGSDVAREAADMVLRDDSFASIVAAMHQGRVIFGNIR
                     KFVCYLMSCNLSEILVIGAGAVSGLPLPLLPLQILYLNLVTDVFPAFALGAGEGEADI
                     MERPPRDPAEPILGRRHWAGVIGYGALIAAATLGAFLIALARPEGGEEAAVTVAFLTL
                     AFAQLWHVFNMREAGSPLFANEITRNGYVWGALALCVGLMAVAVGVPPVAEVLGLAAP
                     DLRGWLLALALSLVPLAAGQLLKLAPRRRRR"
     gene            complement(24923..>26536)
                     /locus_tag="VGB88_01825"
     CDS             complement(24923..>26536)
                     /locus_tag="VGB88_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX9646205.1"
                     /translation="RLLGAHARTWRYLWEQFGIDVDVDGSPSNRMVATLRLHLFHLLQ
                     TASLNTIGLDAGVPARGLTGEAYRGHVFWDELFIFPTLTLRMPEITRALMMYRYRRLG
                     EAREAAREAGFAGAMFPWQSASDGRETSQLHHLNPKSGRWTADNSALQRHVNAAIAYN
                     IWQYLQVTGDVEFLAFYGAEVIYEIARFWTSIARLDEESGRYHIRGVMGPDEYHEAYP
                     DAERAGLDDNAYTNVMAAWVLCRALELRDILPADQQRRLCDKLGLSADEFETWDAISR
                     NMFVPFHDDGIISQFAGYEDLAEFDWDGYRQRYGNIQRLDRILEAENDSANRYKASKQ
                     ADVLMLFYLFSSEELRELFERQGYPFVYETIPRNIEYYLQRTSDGSTLSRVVHSWVLA
                     RWDRPGSWRLFHEALNSDIEDIQGGTTAEGVHLGAMAGTVDLVQRCYSGIEPRAGVLH
                     FNPRLPSTLTRLRFRIRYRGHALALEITHRRLRIAAVECAAEPITVGFKDQRHRLAGG
                     GTLNLDLDGAEEPDSVRDPVSGAGAAAQA"
BASE COUNT         4041 a         9088 c         9487 g         3920 t
ORIGIN      
        1 tgcgccggcg cgggtgcggg cactcggatc ggggcgcgcc gaggtggtgt tcgagtcgcc
       61 gcaaaaggcg atcgcgcccg gccaggcggc ggtggtctac cagggctcgc ggctcatggg
      121 cggcggctgg atcgagcgcc ccgcggcgcc ggccgcctga gcccgcccgc gccttgacac
      181 tgcccgggcc ccgccgtaga agcgggctcc cggggcgcga ccccactggt ggcggggtag
      241 ctcagttggt tagagcagcg gaatcataat ccgcgtgtcg ggggttcaaa tccctccccc
      301 gctaccaatc agatcaagtg cattagagat tgccgaccgg ccgctgcgcg ggcgcctttc
      361 tctcagcggc ggaccgccgg ggtctcgatc gcgaggccct gcgcgcgcca cgcgagataa
      421 agctcgagcg cgacataggc cggcgcgccg agcgggcccg gctgggcgcg caccccgata
      481 tcgcatgagc gcagccgccg gtgcagcgag cccatcccct gccactccag gcgatagatc
      541 ggatagccgg tggggtggcc ctggctgagc agctcgccgc gcaggcgctc gccccaatgg
      601 tcgtcatggc agtggctgca ggcgaggttg agttgaccct ggcgcgtggt gtaggcgctg
      661 cggccggcct cgaagaacgg ccgcgccggg ccgtcgatgg cgacccggat cggcaggccg
      721 cgagattggt acgcgaccag cgccgaaagc gcgagcagct ccgccgagtc gtacgccagc
      781 gcgggcgcgc cctggcgctc ggcccggcag cggttgatcc gcgcctcgag gttgagcagc
      841 tcgccggtcc cggcatcgac cgccgggtag cgcgcggcga cgccggccat gccggcggcg
      901 gggtcgccat ggcagtcggc gcaggcctgt ccggtcgccc cggccgcctc gcgccacaga
      961 ctctcgccgc gcccgaccca caacagcccc ggattgtcga actcgtcgtc ctgcatggcc
     1021 cgggtcgccg gcgactggaa atgatagccc gagcgcagct cgtcgggtgc gatcggcggc
     1081 gtcggcgcat cggcggcgac cgcgcgggcg agggtcgccg caaccgcgac cacggcggcg
     1141 gcgatgccgg ccggccgcct catgtgacgg tgatcgcggc cgtctcagtg tgggtcacgt
     1201 cgtgatcgtc ggtccaggcg aaggcgagct cgccggactc ggtcgccacg gtgaaaaact
     1261 tgagcaacgg attggccgcg atcgccggaa acagctcggc acggaacacc tcgacgccgt
     1321 tgtaggtgca gacgaagctg cggatgatgt cgcgcggcac cgcccgcccg acgctgtccg
     1381 ggcgatggcc ggtctccatc ggatgctgga tcatcgtgcg gatctcgatg acctcgccct
     1441 tcttcgcctg ggcaggcacg gcaagccggg tgttcgccat ggcccccgcc ctacgccggc
     1501 tcgatgcacg ccgcgagcgc caccacgacc tcgacctcgg cgctgcgcaa gctgccgtcg
     1561 gagagacgcg ccagggcgac cacccgctgg ctgccggcga gccggatgcg ggtcgagacc
     1621 tgggccctgc cggcgcgcgc gccgagatag aagtgcgcga cgttgggcag cgggttgtgc
     1681 tcggtgaaca catggatcga ggcgacgtgg tcggcatcgg tcatcgggct gtcgaccgtg
     1741 accgtcagcg gcaccgcgtt gccgttctcg gagagctgcg gaacctcgag caccatgccg
     1801 cccggcgtca ccggcgaatc gcccgccacc tccgcgatcg ccgcggccat gctttccggg
     1861 gccccgccgg ccgcgcccgg aagcagcgca accgcggcca gcccggccgc gccggccagc
     1921 acctgccggc gggtcgcccc ggccggccgc ggacgggttc gtgcgggccg cgcggtcatt
     1981 gcctatcctc gcctgcgcgc ggcgcgcgca gggtaagcaa gtacgcgacg acatcctcga
     2041 cctgcgcggc cgacagcacc gccttgccgc ggtactcgtc ggccaccctt tccagaccgt
     2101 cgatgcggtg ataggccggc atcgcgctgt cggcattgac cagggtcgca tcgaccacgc
     2161 gcaggcgcat ctgcccggcg gtcagccgcc cggccaggcc ggcgagatcc gggccgacgg
     2221 tgccctgaaa cggctcgtcg gcgatcggca gcgcatgaca gatcagacag ttgccaagct
     2281 cacgatcgcg cacgatgtcc tccccgcgcg cggggtcgcc gggcgcggcg gtgagcggcc
     2341 gtgggatctc gtcctcggcc acggtgtagg cggcgaccga ttccgccgag cccgccggcc
     2401 cgccgcccag agccgcgccg aggccggcca gcaacagacc gcggcgaacc ggcctgccgc
     2461 cgcgcggcac tataccgccg ccagatcgtg gtggcgcaac gggaacgacc ggacacgctt
     2521 gccggaggcg gcgaagatcg cattaagcac cgccggcgcg gcgaccgaga tggtcggctc
     2581 gccgacaccg ccccagaagc cgccagacgg catcacgatg gtctcgacct tcggcatctc
     2641 ggccatgcgc agcaccgggt agctgtcgaa gttgtcctgc tccatgcggc cgtccttgac
     2701 cgtgcactcg ccgtagagca tggccgacag cccgtagacg aacgagccct cgacctgggc
     2761 cgcaatctgg tccgggttga ccgcgtggcc ggggtcggtc gcggcgacga tgcggtggat
     2821 cctgagagcg ccatcggccg cgatagagac ctcggcgcac gacgcggtgt agctcgcgaa
     2881 ccccatgtgc tggcacaggc cgcgatagac gcccgccggc gccggcgtgc cccagccggc
     2941 cttgtccgcc accgcctcga ggaccgcgag atgcttcggg tggttcgcca tcagcttgcg
     3001 ccggaatgcg agcggatcct ggccggccgc gtgggcgagc tcgtccatga agcattcgag
     3061 atagatggcg ttctggttgt ggttgacgcc gcgccagaag cccggcggca catgggtgtt
     3121 gcgcatggca tggtcgatca gaaggttcgg gatggtgtag ccgaaggtcc cgtcgccgct
     3181 cggcatcagg ccctggaaca ccaccggatc gcgcccgtct tcgaggcgct gcgggaacac
     3241 cgcggcgagg atcgactggc ccgatatgcg catgtgcagc gcggtcaggt tgccgtccgt
     3301 atcgaagccg ccgataagct tggcctgcgt gaccgggtga tattggccgt ggagcatgtc
     3361 ttcctcgcgc gaccagatca gcttgatcgg cgtgcccggc atctccttgg caatggccac
     3421 cgcctgatgg acatagtcgt ggaacgctcc ccggcggcca aagccgccgc cgagatggat
     3481 cttgtgcacg tcgcacttct cgatcggcag gccggaggcg gcggtcgccg cggcgagcgc
     3541 cgcctcgccg ttctgcgtcg gcgtccacac ctcgcagcga tcgccggtaa acagcgcggt
     3601 cgcgttcatc ggctccatgg tggcgtggtt ctggaacgga tagctgtaga ccgccgcgac
     3661 cgtcttcgcg gcgccggcaa gcgcggcgcg cgcgtcgccc acctcatggc cgacgaacgc
     3721 ctcctctgca tcgagggcct gctccaatat cgcggcgatg gtcgcgctcg agacgccgag
     3781 gttctcgccc tcgtcccaca ccaccggcaa tgcatcgagc gcggtcttgg cgtgccacca
     3841 ggtgtcggcg accaccgcca ccgcggactc gcccacgcga acgaccctcc gcacccccgg
     3901 catgtccgcg accttggccg cgtcgaagct gcgcagccag ccgccgaaca ccgggcaatc
     3961 cttgatcgcc gcgttgagca tgccgggctg cttgaggtcg atgccgtaga cctgtgcgcc
     4021 ggttagcttc ggcgcggtgt cgagccgctt gaccgacttg ccggcgatct tccattgcat
     4081 tgggtccttg agcgcgatct cggtcggcgg cggaatcgcg gccgcggccc gggcgacctt
     4141 gccgaaggtg gtcgtgcgcc cgctcgggcc gtgcgtaatg acactgttgg cggcgctgca
     4201 ctcggacgcc ggcacctgcc actcggcggc ggccgcctgg atcagcatct gccgcgccgc
     4261 ggcgccgccc cggcgcacgt agtcgtggga ttcgcggatg ccgcggctgc cgccggtcga
     4321 gaaattgccc cacacgcggt cgcgcgcaag gttctggccc ggcgtcggat actcggtggt
     4381 gaccttcgac caatcgcact cgagctcctc ggcgacgagc tgggcgaggc cggtgagcgt
     4441 cccttgcccc atttccgagc gggcgatgcg gatcaccacg gtgtcgtcgg gcttgacgac
     4501 cacccaggcg ttgacctcgt cagccatggc gtcggcggcc ttgccgggca gcggcacgtg
     4561 aaatccgagc gagaggccgc cgaccgcggc ggcggagccg acgatgaagc tgcggcgtga
     4621 aagttcgggg gcgaccgcca tcattccctc ctgcaaatcg aaaccgtcga caagtccggc
     4681 acagtggccg gcaccgctca gccgcccgcg gcagccagat gaatcgcggc gcgcacgcga
     4741 ttgtaggtgc cgcagcggca gatgttggtg atgccggcgt cgatgtcggc atcggtcggt
     4801 gccggcgcgc gccacagcag cgccgcggcc gccatgatca tgcccgactg gcaatagccg
     4861 cattgcggca cgtcgagcgc gagccaggcc ttctgcaccg ggtggctggc gtccggcgac
     4921 agcccctcga tggtggtgat cgcggcgttc tcgaccgccg ccaccggggt gaggcaggac
     4981 agtgtggcct ggccgtcgac atggacggtg caggcgccgc actggccgat gccgcagccg
     5041 tatttggtgc cggtcagtcc gagctgctcg cgcagcaccc agagcagcgg cgtttcgggg
     5101 tcgacatcga cggcgcgcgc ttggccgttg aggttgaggt tcaccatgcg aatatcctcc
     5161 cggcggcgag ggcaacgagg cccggatggc tgtgcgtcgg cggccacgtt cgcagccgag
     5221 gcgcagcccg gcatccgagc ccagcagttg ttcgctcagt acccattttc aaaatccgtc
     5281 accgcgtcgt catcgtcgac ggcgcaaatc agtatagcgc gattcagcaa gagtgtccta
     5341 cccagctgaa ttccgacgcc gcagaacgct atcagaattc ggcccgatcg gcgaccaaaa
     5401 acggactcgg cgcgcgcttc gacgccgcag gcatgggcac ggggcggcgc cttttcgccc
     5461 gcgggcggtt cgtggtatcg tgactgcccc gacaatattt cacaggagtg cgccatggcg
     5521 tcgctgcggt tcggtggcat ggggtgggag cgcgacatcc ccgacttccg ggactactcg
     5581 gcggactctg ccgacgtcaa atccgtgctg gcgaagtcga agcccttgaa ggcggccgcc
     5641 aaggccgcgc cggccaaggt ggacctgcgc gaatggtgcc cgccgatcga ggatcagggc
     5701 aacatcggct cgtgcaccgc caatgccggg gtgggcatgc tcgaatattt cgagcgccgc
     5761 gcgttcggca agcacctcga cggctcgcgc ctgttcctct acaaggcgac ccgcaacctg
     5821 ctcggctggg agggcgacca gggcgcgtac ctgcgcgaca cgatgaaggc catgtgcctg
     5881 ttcggcgtgc cgccggaaag ctactggccg taccgcacgc agcgtttcga cgacgagccg
     5941 acgccgttct gctacgcgtt cgcgcaaagc tacaaggcga tgcagtacta ccgcctcgac
     6001 ccggcggggt cgccggccgc gcaggtgctg gccgacgtca agacctacct ggcggcgaag
     6061 ctgccggcga tgttcggctt caccgtctac agctcgatgc cggggatcgg cgccggcacc
     6121 ggcgacatcc cgtttccgag ctcgggcgac cggcgcgagg gcggccacgc ggtgctcgcg
     6181 gtcggctacg acgacaaccg gcgcatcgcc agcgagaagg gtgcgctcct gatccgtaac
     6241 tcgtggggcg aggagtgggg cgacaagggc tacggctgga ttccctacac ctacgtccag
     6301 cgcgggctcg ccgtggactt ctggtcgctg gtgcgggccg agttcgtcga caccgacctg
     6361 ttcaagtaac gccgggtcac gcccggccgg cgtgcttggc gaggaacggc aggaagcgcg
     6421 gaaacacgtc gccggccgcg tccttgccca tgaacgggtc ctggtggccg tagccgtcga
     6481 ggatcaacag ctcgtggcgg ttgccggggg cgagctccat gagccggttg aagcacagga
     6541 tgttggaatc ggtgaacacg cggttgttcc ggccggtcat gaacaagacc ggagtgccga
     6601 tctccctggc atgggcgagg tagttgtcgg gcagggcggc gtgggcgtcc ttgtccgggt
     6661 acatcctgac cgcggtgttg ccggcgcgca ccatcttgag cacgtggcgg tggtagttga
     6721 ggctggtgcc gccgtacagg tcgccgccgc gcgcgtgggt gaccggcaac aagttgtcgt
     6781 gctcataaag cgccggccaa ccggtgcccc acatcaggct cagcatgtgg caggcgggca
     6841 cgtcgcactc gcggtggaac cgcgagaccg cccagttgag cgccttgccg aaggtcagcg
     6901 tcgggtcgtc ggcccagcgc gggctgacat agggaacgct gagcacgttc tccaagagga
     6961 acggaaacat cgcgcccttg atgcgcgacc aggccggcac gcgcggggtc agcgcgaccg
     7021 agttggcgat cacgctggcg atgccgtcga cctgcttggc gaacaggctc atcacgaacg
     7081 acatcgagcc gaggcagtgg cagatcacgt ggatgcgcct ggcgccgctg atccggcgca
     7141 ccgcctcgat tgcggcgggg tggtcgtaga gggcgcagtc gtccatcgag tagcggtgcg
     7201 gcgccaggtt gtagacgtgc cggttgctca tgcggaaatc gagcgtgaac acgtcgccga
     7261 agccctgggt gtgcagccag ctgaccaggt tctggtgctc gggcatgatg aacatgtcgg
     7321 acgatgtggt gagcccgtgg atcaccacca ccacgtcgtc gcagtcggcc ttccagaagc
     7381 ggagcatgct gatgctgagc ttgtccgccg tcggcacgta atgggtggtg atgcgggcgt
     7441 ccttgacgcc gtcgagggtg tagagcgcga tctcgcggtg catggccggc ctatccgctg
     7501 agtattccct gggcgacgtg ctcggcgagc gcggcgatgg tgcgcgaggg gttgagcccg
     7561 atcgccttgg gcaggatggc gccgtcggcg acgtagagcc cggggtggcc gaacacctcg
     7621 ccgcccgcgc cgaccacgcc gtcggccggc ccgctgccga gcgcgcagcc gcccaaggga
     7681 tggggcgtga tcaggtcctt ggcccagcgc caatagagcg gctcgatggg cacgccgccg
     7741 gtcgcgcgcg acagcgcctt gtgcatgtcg accatggcct ggatggtcgc ctccgagtcg
     7801 gcgacgtccc agtcgagcag gatcttcctc ggcccgaacg gccaccacca gcgccggccc
     7861 agatacagcc gcccgttcga ggcgtcggcc gcctggccga accacggcat gacgcagtcg
     7921 agcgggtcgc cgccgcgcgc ctcctttgcg agccgcgcaa acgccttgcc gagcacgccg
     7981 ccgaggcgtc gcccgctctg tgccagctcg ccgatggtgt tgccgatggc gtcgggaaag
     8041 ccgccgtcct cgacgaagaa ccgccgccca tcccggctac cgtcgaggaa gtcgatggcc
     8101 gcggtgatgg tcggcccgcg cgtcggcgag acgcggcggt cgcggtagac ggcgggggtg
     8161 aggaaatcgc cgttggagct ccacccgagc cccagccgcc ggctgacatt gggcaaggtg
     8221 cgatagtcgt cgcggcagcg cagcaacagc tcggtcgagc cgagcgagcc ggcggcgaga
     8281 acgacgatgc gggcggcgca gtcgtcggcg atcaggcggc cgtcctcgat gcggtcgacg
     8341 ccgaccgagt agccgtcgtc gagcggcgcg atcgagcgca cgacgtgcag cgggcgcagc
     8401 tcggcgccgc tcgcgcgcgc cgccgccaga tagttgaggt cgagcgtgtt cttggcgccg
     8461 acccggcagc cgatgtcgca gttgccgcag tggatgcagg tgccctggac ctggccgtgg
     8521 gcattggtcc agcgcttcga ccacttggca tcgtgcgcat cctcgaggcc gtagtgccag
     8581 tcgtcgctga aggtgacggc gagcggcagc ttgcgcaccc ggtcgccatg cccgagggcc
     8641 tcggcgccgt cgcgcatcag cttggtgcgc tcggtgagct ggccgtcggg cagctcgtgg
     8701 acgtcgagca tccgcccgac ctcggcgtaa tgcggcgcca gcgactcgta gctgatgccg
     8761 ggcggccagc cccgctcgaa cgcccacggc tcggcctcga ccgagacgtt ggcgtagatc
     8821 agcgagccgc cgccgacgcc cgcgccctgg atcacggtca tgtggccgaa gaagcgcgcg
     8881 tcgatccagc cgttgtgccg ctccggatgg tcgtcgtccc agagccaggc gtcgccgggg
     8941 gcgcggggat agtcctcggc gcgccattcg cgaccgcgct cgagcaccag caccttgcgc
     9001 ccggcttcgg ccagccggca cgccaccggc gcgccgccga accccgagcc gaccacgatc
     9061 gcgtcgtagc gccggaccat ggccggttat cgccggccgc ggccggtgaa cgacttcagc
     9121 atctcgggcc cgtagctgtg ccacagctcg cccatgaaga agacgccgaa cttggtgagc
     9181 gtctcggcga tctcgagcgg cgagccggcg ccggtcaccc gcaccgtcgc aagcagatcg
     9241 ccgagatcgc gcaccccgag gccgagaatg ccggcaccga ccaccggccc gtccttgccc
     9301 gctccctggt gcaaggtggt gtagagggtg gtggtgtccg accacaggtc gaagccgcgg
     9361 tcgtcgcgga cttccttgcg cccggccacg taatacggct tgccggcggc gctgatcgcg
     9421 agctcgtaga ccatcagctt gagctcgggc tggccggacg gcgagaacag gttgaacacc
     9481 ccgtcatgcg ccgtgagcgc gacgccgagc ggcgcgaagt cgaccgagcc cgcaagctcg
     9541 cccgcatggt cgggatcggc cacgaacgcg tcgagatcgg cgatcgcgac gcgcgcgttc
     9601 atggtcagcc gggtgccgcg ccgggcaccg gtcgcgcggc cgcgctccgg gtcctgctcg
     9661 tcgagcgcga acggcccggc catggtctcg ctgaacgtga tcccgcgcat cacccggacc
     9721 tcccggtcac cagggcgcga gctcaagctt ggcgatcagc cggtcggtcg gccggttgag
     9781 ccagtcgcgc ttgcggatca gctcgccctc ggtgttgaac gggctgacgg tgatgctggc
     9841 gctcgcgcgg tcgacgtcga tgcggcagta gttgtcgtcc tgggtgaagt tccaggcctg
     9901 gtactccatc gtctcggcct ctgagatcag gaacgtgtcg cgctggtcgg cctgcgtcga
     9961 gtcgtggacg tagttggacg ggtcgccgtc ggcgaagaag aacggccaat agaacgccga
    10021 ggaggtgatc gagaaggcgc gcagcgcctc ggccgtcggc gcgccggcga agcgcatccg
    10081 ggcgacgttc gagcaatgga tgtcgcccga caggaacacc acgttctgga ccgcgttgtc
    10141 gatcaggcat ctgaggatcg cgcgccgggt cacggggaag gccggccagg aatcgctcgc
    10201 ctgcatccac tggatcatct ggtgccgcgc ctcatgcaag tgcggcgcat ccaagcgtcc
    10261 ggtgcgcgcg ctcatcgggc tcggcacgaa cacgctcgag ctgaccacga acttgggcag
    10321 gttgccgccc tgctgctgtc gctcgaccag ccagcgcagc agccgttcga gctggctcgg
    10381 ctcctcgccg cccatcgagg ggcggcccag catgtggttg tcgtcgagac tgtcctcgac
    10441 gtcatcaatg aagcgctggg tgcgcgtgtc gagcacgaag aacggatatc cgccgcagtc
    10501 gaaggtgcaa tagaggcgcc ggccgaagct gcgcgggccg tggctccact ggtagctcat
    10561 ataggcgccg atcgcgatgt tgaagacgcg gcgcgccgcc gccttgctga gcctgtcctg
    10621 ggtccagttg tcctcgatct cgtggtcgtc gaggatcatg taggtcggca tgccgcgcaa
    10681 cagccggctc atgttgggcg agccgaacgc ggtcaggtag cgttgctgga actcctcgaa
    10741 ggtatcggca aggcccaacg ggacgtgccg gttgagcatg tcggcgtaga tctggtcgcc
    10801 gaccatcagc gcgaactccg cccgccggcg cttcggatcg cccacctcgc ccgcggcttc
    10861 cgcgcgcagc ggcccgaaga tggtatcggc ctccttggcc ttccacagca gtcccggata
    10921 gcggcatgag ccgaggatga aggaaagctc gtcggcggcc gccgcctcgg ccttggggaa
    10981 ggtgaagaac tcggcgacac aacggctgcg gtcgcgcttc agcaacgtct cgaggtcgga
    11041 cctcaggttg tccggatcgg gcagccgatg tgccaggtag tcgtcggtga ccgtctcgtc
    11101 gtcggcgaac ggatcgtcga tgaccagggt gccgaccacc gccgcatgcc gcgtcgccgg
    11161 cgcgagcgtc gccggcccct tgctccgccc gcgccgcgta tgcgggtccc agtcgggcac
    11221 gatgccgacc tcgtcaccga gattgagggt gccggtgcgg tcgaactcgc ggtgcaggcg
    11281 gaagtagtag acgtcgggct tcgcgatcgg cttgccgttc cgctcgcaaa gggcgatgac
    11341 gccgatggtg cggcgggcgg aagccagctg cgcgccctga tcctccggat cgccggcgcg
    11401 aatccagatg cggcaggatg tcggcgtggt gtgtccgacg atggggccga gcccgggaaa
    11461 ccgaaggatg ctcatgcctc gcccccgccg cggaacacgc gatccagcag atcactttca
    11521 ctgccaaatt gcaaactcgc cggtcgcgat cacattttgt tgctgtgctc ctctatacgt
    11581 gcacaagcag ttggcgtgac ccgggatgca gacgtcgttc gagcgctgcc tccgccaggg
    11641 aaaacccgga cgtcgctcat gatcgcacgt gccgcaccgt ggactgccag ggcctgtctc
    11701 cgccacggtt cgcgtcagcg attcgacccc gccgcgggcg gtgtgacgca cgtcatcgac
    11761 agggccagga cggcgatata tcgaaggggc gtcgcgcaat ccaggtccgc cactgcggga
    11821 gtggcggcta agcggggacc gagtgcggac tgatacacgt cgaaccgttg gcagcatgcc
    11881 ccagagaggc acctacgtgc cgcccgagga cacggaagcg cgaacggatc atggcgatcc
    11941 ggcgcgacgc cggagggccg atcgcatggc gaacatcgac gccgtccgct ggatcgaggt
    12001 gaccgatcga tcgacgccgg tcggctaccg cgagttcctg gatccggtct ccgggtccag
    12061 atgcgtgatc cgtgcccaca cggtcgacga ggatggccgg ccgacgggag ccgcccacgg
    12121 caacgaccct gccggcggag tcggccccgg gctggaccag gcctcgggcg cgaacggcgc
    12181 gcgatttttc atctacagtg tggtggcaac cggcctggcc ctggccggca tcgcgatcac
    12241 ggtgtggcag gcgctcaaga tgttcggcgg cgattcgtga tcgactgccg ccggcaggcg
    12301 gccgcggcgg gctcagccgg cgctcgtgcg ccgcagcgcc tcgagcagtc cctccatgcc
    12361 ctcgcgctgt accagtgagc cgaactcgtc gcgcttggtc acgatcaggc tcaggccctc
    12421 gaccgcgacg tcgacgatgc gcagcgtctc ctgctcccgg cgcacgcgga agtcgaccgg
    12481 cagcggcggg ccgtcggggc ggatgatgtc gagccggacc agcacgtcgc cgccggacag
    12541 ttgtgtctgg gcgcggatct cgaacgtctc gcccgagtaa tccgaaaatt gcgtcgcgta
    12601 gatgccgccc acatatcgtc cgaacagccc gagatattcg tgctgctggc tttcggtggc
    12661 ggccttccag tgccggccga gaacgaactt ggccaccacc ggcacgtcga aggcttccgt
    12721 gaacagctcc tgcagccggc cttgcagctc gccgcgcggc aggtcccgtt tgagttccgt
    12781 cagcaggcgg tcgccgaggt cgctgacgaa gtccgtcggc gtgcgcgccg ggtcggcgcc
    12841 ggcggagccg gtcaccaaca gcaacaccgc cgccagcgcg gccacgaatt gccttaccat
    12901 tacgggtacc cccgcaacaa ccttggcggt ccggcgcacg aggctcagcc gccaccgccg
    12961 gccggcgccg ccgagaccga cgattcccaa attggacctc gggcgtgccg cttcaagtgc
    13021 gaatcgggcc gtaccgtagc gtcgcccggc cggcgtcgcc agcgacaagg tcgtgacatt
    13081 ttcggggctc gggatcggcg ggcgatcact cggcggcgct gggctgcctc agcaccggca
    13141 actctgccac cgcgtccgag cgcttgccgg tgacgtaggt gtacacgcac ggaatcacga
    13201 acagggtgaa gaacgtgccg accagaagcc cgccgacgat cacccagccg atgtcctggc
    13261 ggctctgggc gccggcgccc tcggcgagcg ccagcggcac cgcgccgagg accatggcgc
    13321 cggtggtcat caggatcggc cggaagcgga gcgtcgccgc ctcgagcacc gcttccctgg
    13381 tctcgcgccc acgcgcgcgg atctgattgg cgaactcgac gatcaggatg ccgtgcttgg
    13441 tgatcaggcc gatcaaggtg accaggccga cctggctgta gatgttgagc gtgccgccgg
    13501 tcaggtggag cgcgaccagc gccccggtca tcgacagcgg cactgtcaac atgatcacga
    13561 acggatcgac gaagctctcg aactgcgcgg cgagcaccag gtagatgaac gcgagggcga
    13621 gcgcgaaggt caggtagatg tccgcgctcg agcgcttgaa ctcgcgcgac tggcccgaat
    13681 agtcgatctg cgtgtcgggc ggcacgatct cgcgcgccgc cgtctcgagg aagccgagcc
    13741 cttccgccag tgaggtgccg ggcgccaggt tggcggtcac cttggccgcg cgcagctgat
    13801 tgaaatggtt gagctccttc ggcgccaccg tctcctcgat cgagaccagg ttggacagct
    13861 gcaccagctg gccgccgtcg ctcttgacat agatgcggtg caggtcgtcg gggttggtgc
    13921 ggtcgacgtc ggcgacctgg acgatgacgt cgtactgctc tccgtcctgc ttgtagcggg
    13981 tgacctggcg cccgccgagc agggtctcga cggtgcgtcc gagctgggcg acgtcgatgc
    14041 cgagcgaggc gaccttgtcg cgcttgacgt cgaccctgag ctgcggcgta ttgagcttga
    14101 ggtcggtgtc gatgttggcg aggccgggat agtcctccgc ccgcgccatg acctggtcga
    14161 cccatcccgc cagctcgtca taggagcgga tcgtcccgat cacgatctcg accggcttgg
    14221 aatggcgggc ctgcccgagc ggcggcggca ggatcgggaa cgcaattacc ccggggatct
    14281 gcgacgcctt gggcccgacc tcggcagcga tctcgcccgc cgagcgctcg cgctcgtccc
    14341 acggcttgag attgaggaag gtgatccctt gcgagaccac gggatagccc gagacgacga
    14401 agtaatgcgc gacctcgggg atgctctcga cgatcggctc gaagcggcgc gcgtagcggt
    14461 cggtgtagcc gatggtcgcg ccctcgggcg cgaggtagaa caggcggatg atgccctggt
    14521 cctcgtaggg cgcgagctcg gacgtcaggc tcatataggc cggcacgccg gcgccggcga
    14581 cgccgaggcc gatcagcaat atcagcgggc gcacccgcaa gcccaggcgc agtgtcgcgc
    14641 gatagccggc ggtgagccca ttgagggcgg cctcgccgag gcgaaagagc aggccgtgcc
    14701 ttacgccatg cggcttgaga agaagtgagc acatcatcgg cgacaaggtc agcgcgacga
    14761 atcccgagac cgcgacggcg cccgccagcg cccaggcgaa ttcggtgaac agccggccgg
    14821 tgttgccggt catgaatccg atcggggcgt agaccgcgac cagggtcaat gtcatggcaa
    14881 cgatcgcgaa gccgatctcg cggctgccat ccagcgccgc ccgcagcggc ggctggccgc
    14941 gctcgacatg gcggtagatg ttctcgagca tgacgatggc gtcgtcgacc accagcccga
    15001 tggccagcac catcgacaat agggtcaagg tgttgatggt gaatccgaac agcgccatga
    15061 gcgcgcaggc gccgatcagc gagatcggga tggtcacgat cggcaccagc gtcgcgcgca
    15121 ccgagcgcag gaacaggaag atgatcagga ccacgaggac caccgcctcg ccgatggtgg
    15181 cgtagacgtt gtcgatcgat gcgttgatga agatcgtggt gtcgtaggcg acggcgacct
    15241 tcatgccctc cggcaaggtc tcgcggatcg agggcagcgc gccgtagatc gcgttcgaga
    15301 cctcgagcgg attggcggtc gcctgcttga tcacgccgag cgcgatcgcg gtctcgccgt
    15361 tgtagcggac tacgcggcgt tcttcctggg cgccgatctc ggcgtggccg acgtcggcga
    15421 ggcgcaccag ataaccgccc gagcgcttga gcaccaggtc gtcgaactcc gccggggtct
    15481 gcagatcggt ctcggagagc accgagaact cgcggtcgac gctctcgatc cgcccggcgg
    15541 gaatctccac gttctggctg cgcagcgcat cctcgacgtc gaccggggtc aggccgaggc
    15601 cggccagccg gccggggtcg agccagatgc gcatcgagta gcggcgctcg ccgaagatgc
    15661 tcacctcggc gacgccgggc aggatctgca ggcggtcctt gacgaagcgg tcggcatagt
    15721 cggtgatctg gggcggcgag tgccggtcgc tggtgaaggc gagatacatc atcgcctggg
    15781 cgtcggcctc gaccttctgg atcaccggct cgtcgatgtc gtcgggcagc tgggcgcgca
    15841 cccggccgac ccggtcgcgg acgtcggcgg cggccgagtc cggctcacgg tcgagaccga
    15901 aggtgagcgt gatctggctc tgctcctggc ggctgatcga ggtcatgtag tcgatgccct
    15961 cgatgccggc cagcgagtcc tcgagcacct tggtgacctg ggattcgacg atcttggcgt
    16021 cggcgccgcg gtacgaggtc tggacgttga ccaccggcgc gtcgatgtcc gggtactcgc
    16081 ggacattgag gcgctggtag ctgacaatgc cgaccagggt cagcacgacg ctcatcaccg
    16141 tcgccagcac cggccggcgg atgcacagct cggagatctt cacgacgatg cgtcgacgac
    16201 gccgggcgcc ggctcctggt cgggcgcgcc gcgggtctcg atcacccgca ccttggcgcc
    16261 gtcgcgaatc ttggcatggc cggcgaccac gatctcgtcg cccgcctcga ggccgtcgac
    16321 cacctcgacc cggccggtct ggcgcaggcc gagcctgacc tcggtcaata ccgccacccc
    16381 gtcgatgata cggtagacat agcggcggtc gccgcgcggg atcagcgcct cctcgggcac
    16441 caccagcgcc tgctcgcgct cgtcgacgat caggcggacg cgggcgaaca gcccggggcg
    16501 cagatgtccc tgcgggttgg gcagggtggc gcgcaccgac acgctgcgcc cgcccggctc
    16561 gatcagcggg tcgagcgcgt agacggtgcc gacatagctc tcgtcgggaa aggcgtcgag
    16621 atcgacctcg accgcctggc cgacccggat cacccgcagg tagcgctcgg gcagggcgaa
    16681 gtcgagcttg agcgggtcca cgctctcgag gttgacgatc gcctggccgg cgcgcacgta
    16741 gtcgccgacg ctgaccccgc gcaggccgac cagcccggcg aagggcgcgg tgatggtgga
    16801 tttctcgagc cgcgcccggg caagcgcggc ggcggcctcg tcggcgcgca tctgggcgag
    16861 cgactcgtcg agcgtctggc cgctgccatg tcctttctcg aacaacgcct gcgcccggtc
    16921 ataggtgtgc cggctcagct cctggcgcgc ttcggcctgg gcgagctcgg cgcggaagat
    16981 cgagctgtcg aggcggatca gcatctggcc ggtggcgacc ggccggccct cgtcgaagcc
    17041 gatctcggtc aggcgaccgt cgatctcggg agacacgacg accgactcgt tcgacagcag
    17101 gctgccgacc gcggagatcg actcgaccag gcgcgtcggg gcaatgatgg cggactcgac
    17161 cgccaccgcc ggcttggcgt cctgggcgcg cgcgcccggg gcgccgctga gcagcacggc
    17221 aaggcaggcc gccgccgcgg ccattattcg cttgtcaggc acggtgcctc gtttgctaga
    17281 ctggaccgta cagtatgaat tggactcacc cggcccctcg ccgtcaagca gaatcgcggc
    17341 gcggcaccgg cgccggatgc ggcctcgaca aaggccggcc aacgcctaga gtgaggcatc
    17401 atgtcgcttg ccctgtccgc gccgcgcgag ctgtcacgct ccgaccagaa gaaggcagcc
    17461 attatcgagg ccgcgacgca ggtgttcctg cgcgacggct acggccgggc gaacatggac
    17521 tcgatcgccc aggtcgccgg ggtctcgaag cagacgatct atcaccactt cggcaacaag
    17581 tccgacctgt tcggcgcgat catccgcgcc aagtgcgacc gcctgctcga gccgctggag
    17641 atgccgaccg agtcggccat ggacctggaa ggggcgctgg cctcggtcgc ccgccagttc
    17701 atcgacatca tgctggcgcc atcctcgctc gcgctccacc gcgtgttgat gggcgaggtc
    17761 caccggttcc cggagctcgg ccgcatgtcg tatcagatcg ggccggcgcg gatcgtcgcc
    17821 acgatcgcgg cctatctcag ccgccagggc gcggccggcc ggctcgacct gcccgacgcc
    17881 aagttcgcgg ccgagcagtt ctacgccatg ctcaccggcc acctccagct cagggcgctg
    17941 ctcggcgtcg aggccgaccc gtcgccgggt cagatcgagc gcaccatcag gcacgccgtc
    18001 gacaccacgc tgaagatcta cgcgccgcac tagggttcgc gccggtgcga tcacggcgcc
    18061 gcgcgggatc gaccgcgacc ggccgtgtcg caggctcgcc acgccgccgc ccagacatta
    18121 gagtagcgcc gacgcggccc gaggttgacc aaggggaggg gacgaatgac tcggtttgtc
    18181 gtgctggcgc agtttcgggt caagcccggc aagcgggaca gcttcgtcgc gctggccgag
    18241 accgacgcca cggcggcggt ggccaacgag cccggctgcc gccggttcga cgtgctggtc
    18301 ccccacgaca gcaccgaacg cgtcgtgctg tacgagatct acgacgacaa ggcggcgttc
    18361 gacgcgcacc tgaagagccc gcatctggct gcgttccgca agggcatcgc cgacctgatc
    18421 gaggaccggc ggatcgagac cttcgaggtg cacgagcacg ccgacccgtg accgccgagc
    18481 gggcgggggc gcgggcagcg tccgagcggc cggccggtta ctcgtcctcg tcctcggcca
    18541 cggccacgtc tatggtcgcg ccgtcggcca cgtcctggta gaccgccctc ttggcgctgg
    18601 cgcagccggc gatgacggcg gtcgccagcc tggcggcgcc gttgccgtcg gcgcagaccg
    18661 tcaccttggc ggcctcgccg ggcttcatgc gcccctgcca gaccaccgcg ttgtcgcaga
    18721 ccatgtcgcg cacctcgacc ttccattcca tgtcggtcgg gttgttgagg cggacgtgga
    18781 tcgtgtcctt ggccgcggcg ccgagcgcgc cggcaaccgc cagcagcgcg gcggccgcca
    18841 tcccgaacca gactctcacc atcgctctcc ccgagtggtt gtgccttggt tatgggacag
    18901 cataggacac cggcggcggc cggtacagcg ccggcgctca ctggacgatc ggctgggcca
    18961 ggatccacag gctgagcatg gtgtagcaga ccatcagcac cagcatcggc acctggctca
    19021 ggatcgccgc ggcgcggctc gcgaacaggc gcagcgcggc aacgtgcgcc agcaccaccc
    19081 cgatcacgtg gccggtgacg atggcggtca ccgccacgta ccagaccacg cgcgcgttga
    19141 tcaggctgat gtcgatccgg tagcgcgcgg tgccgagcag gtcccagccg aacccgaacg
    19201 ggtcggaggc gagcgggatc gcaagctggc ccgcgatcgc caggaacgac agatagtggg
    19261 cgaagtgata gccgaccgcg atcggcacca gggtgaacgc gaaccggccg gcgagcgccc
    19321 acaggtcgta atgcgcgggc ccgcccggcg cggcggcgag cggcgcgagg cgcgccatga
    19381 tccagacgaa gaccagaaag aacgcgagca cggcgatcgg gaacagcacc agcgcgacgg
    19441 tctcgatccc cttggcggcg ttgccgaaca catcgcgcat cacgaacagc gccggcgcca
    19501 gcgtctcgca cgagaacgcg gcgtcgaccg tggcgctcca cagcggcgtc tccatcaggc
    19561 cgtcgaaggt caccgtcgac agcatcagca cgacgaagca aaccatcgag cgcgacgccg
    19621 gcgcatccac caaaagcccg gcgccgtagg gacgcagctc gagctccgcg ccgccgcccg
    19681 gcgccggggc gatcggcgcg aagcggccga acacggaaaa ggcgagggtg aacgcctcgc
    19741 cgccgccgag ccaggcgtcg cggccgaagg caaccatcgc cgccaaggtc agcacgctgt
    19801 aggcgaggat caccgccgcc agtgccgccg gcacctcgcc gtgcttccag atcagctcga
    19861 tccacgcgaa gccggcgaac agcgccaccg ccggccacgc gccgagcgcg cgcgggtagc
    19921 ggagcggcgg cgccggcggc ggccggcggg ccaaccgcgt ccatgcccag gcgccgagat
    19981 cgtagagggt cgcccacggg ttgagcgccg gccacgggtt ggcgaccagc acgcacacga
    20041 acaccagccc gacccaccac agcacccaca ccagggtcgg ggcgatgttg cggatcgggc
    20101 tctggacgcc ggcgagtccg gcggcgacga ccagcacgag caccgccagg ccgagcgcgc
    20161 gcagaagagg aaccatggtc ttggcggccg cgccgaccgg aaggcgcggc cagggccgcc
    20221 tccggccggg gccggcgcgc cggcgcgcgg cgacggcggc gagggcgaat gacaggccga
    20281 ccaccagcgc cgccgcggcc agccagtacg acagcggcag cggcagatcg tagcgctggc
    20341 cgaagccgtg cgcctgcgcc gggccggcgg cgaggacgcc ggcgagcgcg agccgcaggc
    20401 tacggcgcga ccggcgcgcg accgccacgg cagctagcgg ggatggacct cgacatagag
    20461 cagggcggac tcgtcgtgct cctcgccgtg gtcgtcgccg aagccgtgca cggtgaccgg
    20521 gaagcgaccg gtggcattgg cgtcgaactc gatcgccgtg acctcgcccg gcaccagccg
    20581 ggcgggcagg tcgtagccgt gcaggtggat ctcggccggc tcgtcgccgg tccagcgcag
    20641 gaccacgtgc tcgccctcgg tgacgcgcac cacctggtcg ccgaccaccg cgcggtctgc
    20701 aatcgtgacg tcgcgcgtca cggtcgcgcc ctcggcgcgg gtactgcccg ccacgcagat
    20761 cagcagcagc ccggcgacga gataggcacc ggcgaggcgg cggtcacggc gccgccccgc
    20821 ccacttcgac agcggcaagg tcatcgctca catgctgccg gggcccgggc cgttgttgaa
    20881 gtggcccatc gggtggtacc aggcgggcaa cagggtcaac gagttggggt tgaggttctg
    20941 gctcttgaac gcgtcgatcg agtcgaccac ctgctggcgc tccaggtcga ccaccgtgat
    21001 cgagccgtcg ctcatccccg gcaggttgag cagcgagttc tggacgaagg cgaacttctc
    21061 gtcctgggtc agggcgacgt gatgggcgcc gcccgccgcc ggcagggtct tgagtagctt
    21121 cggcgccagc ggcccggcgc tgatgtcgaa gatgtggaac atgccgggat cggcggtggt
    21181 gacgtagagc cggtcgaccg ccttgttgaa gtacatctcg agcggcatcg ccgcgccctc
    21241 ggtcgagaag tcgaacagct cgcggatatc gaagctctcg gtctcggggt tccagaccgc
    21301 ggcggcgagg gtgtgcgcca tcatgttggt gaagtatgcg accggcggat cgctgtcggg
    21361 cacgaacacg atctcgaccg gcgccgcgga gcccttgtcg gaggcgggta ggctcgccag
    21421 atgctcgccg gtgctggccc ggatcacctc gacggtggtg ccgaccgcgc tcatgtcggg
    21481 cttgatgcaa ttgaccacca gcagccggtc gatgctctcg tcgagcgcga tgccgtgcgg
    21541 atacgcgccg ggaagctcga tcagaccggt catctcgtcc ttgaccgcat cgcccaccgc
    21601 gacgttgctc gatcccatgc aggtgacgaa ccagcgcgag ttgtcgtcgg agaagatgat
    21661 gtcctcctgt accttgcagc cgggggtcgc gatcggcctg gcgcggtagg gaaactcgcc
    21721 gaggtcgatg acgtgcagga cctccttgcc gagcgcggtg acgtaggcct tggtcaggtc
    21781 ccggttgtag aacacgtgat gaagcaccgt gtccggcggc atcggcatgt cgatcaggat
    21841 cttgccgtaa tcgggcgagg cccggtcaag ctcgatgatg gcgatgccct cgcgccgcac
    21901 cggcgtcgcg gactgcagct gcagcgactg cagcgactcc tcggccttgg tctcgtagtt
    21961 ggcgatcgcc agcatgtcgg cgcgcgccgc ggccggcgcg gcggcaagag ccaccgtgac
    22021 gacggcgagc gcctctcgca acttgaacat ggtgcaattc ctcccttggc cttgactcgc
    22081 ccgtgccctc ccacggcctc gggctggcct ggtgacaacg gtgataggta atgagtatgc
    22141 catcacggcg ccggcgcctg tgagctagtt cacagccgca ttcacgcggc cggccgacac
    22201 cagagcatag gcgagcggga ccatcttgga tacagcatcg aacacggccc ctgatgccgt
    22261 cgttgggcct gatggtcggg atcgccgcct gtggccgcgg cgccggcgcg gctgatgaac
    22321 gcgcgcgcgc cggcggccgc gatcgccgat gctcatgccg tgcccgcggc cggggtgctg
    22381 agccggctcg gggtcgatcc ggcgcgcggc ctcggctggc gcgaggtcgc ggcgcggcgc
    22441 cggcgctacg ggcgcaaccg gctgccggcg caacctgcgc gcagcctctg gtcgatcctc
    22501 gccgaccagt tcaagagcct ggtggtggtc ctgctcgccg cggcggcggc gttgtcgtgg
    22561 acgctgcgcg aatggaccga gggcacggcg atcatggtcg tgctgctgat caacggcgcg
    22621 atcggcttcg tcaccgagct gcgggcggtg cgctcgatgg aggcgctgcg ccggctcgcg
    22681 accgtcgcca ccagggtcag gcgcgcgggc cgggtgcgca tgcgcccggc gcaagccctg
    22741 gtccccggcg acgtggtggt gatcgagggc ggcgacatcg tcaccgccga cctccgcctg
    22801 gtcgaggccg cgcgcctgca atgcgacgaa tcgacgctca ccggcgagtc ggtgccggtc
    22861 gacaagtcga ccgaggcgct gccgcccgaa gcgccggtga ccgagcgcgc gaacatgctc
    22921 taccgcggca ccgcggtgac ccgcggctcg ggcatcggcg tcgtcgtcgc caccggcgcg
    22981 gccaccgagc tcggccagat ctcggcgctt gcgcaggccg cggcgccgga gacctcgccg
    23041 ctcgagaagc ggctcgaccg cctcggcggg cagctggtgt gggcgacggt ggcgctggtc
    23101 gcggtggtcg gcggcctcgg cgtgctgcgc ggcaacgagc cgctgctcat gatcgagacc
    23161 gcgatcgcgc tcgccgtcgc cgcggtgccc gagggcctgc cgatcgtggc gaccatggcg
    23221 ctggcccggg gcatgtggcg gatggcgcgg cgcaacgtcc tgatcaggcg cctgtcggcg
    23281 gtcgagacgc tcggtgcgac cacggtcgtg ctcaccgaca agaccggcac cttgaccgag
    23341 aatcggatga cggtgacctg gctcgagctc gaccggctcg cggtcgaggt cgggggcgcc
    23401 ggcgaggcgg cattcactgc cggcggcgcc gcgctcgatg cgcgcgccga cccgcgcgcc
    23461 gccatggcgc tgaggatcgc cgtcgcctgc aacaacgccg ccctcgagcc ggtcggcgag
    23521 gacggcgcgg cgcgcggcgt cggcgacccg atggaggtcg cgctcctcct cgccgccgcc
    23581 aaggccggac tcgaccgcgc gacggtgctc cgcgactggc ccgagcagcg cgaggaggcg
    23641 ttcagctccg acgtcaagat gatggcgacg gtgcaccggg ccggcgcggg ctacatggtc
    23701 gcggtcaagg gcgcacccga agcggtgctc gccgccgcga ccgcggtgct gggcgctgac
    23761 ggcccgatcg cgctcgatgc cgcccggcgc gcgcactgga cgcgccgcat cgaccggctg
    23821 gcggcggacg ggctcagggt gctggcgctg gccatgaagg agacggcgga cgccgacgac
    23881 tcgccctacc gcgacctcac cctggtcggg ctggtggcgc tgatcgaccc gccgcgcggc
    23941 gaggtgccgg cggccgtcgc cgcctgccgg cgggcgggcg tgcaggtggt catggtcacc
    24001 ggcgaccatg ccgagaccgc gcgcgccatc gcgcgagcgg tggggatcgg ggacccgggc
    24061 gacgccaccg gcgggggcgg ggtgttcgcg cgggtcacgc ccgagaccaa gctcgatctg
    24121 gtcgcccgct accaggccca aggcgagatc gtcgccatga ccggcgacgg cgtcaacgac
    24181 gccccggcgc tcaggaaagc cgacatcggg gtggcgatgg ggcggcgcgg cagcgacgtc
    24241 gcgcgcgagg ccgccgacat ggtgctgcgc gacgactcgt tcgcctcgat cgtcgccgcc
    24301 atgcatcagg gacgggtcat cttcggcaac atcaggaagt tcgtctgcta tctgatgtcc
    24361 tgcaacctga gcgagatatt ggtgatcggg gcaggagcgg tgtcggggct gccgctgccg
    24421 ttgctgccgc ttcagatcct ctatctcaac ctggtcaccg acgtctttcc ggcgttcgcg
    24481 ctgggcgcgg gcgagggcga ggccgacatc atggagcggc cgccgcgcga cccggcggag
    24541 ccgatcctcg gccggcggca ttgggcgggc gtgatcggct acggggcgct gatcgcggcg
    24601 gcgaccctcg gcgcgttcct gattgcgctc gcccgccccg agggcggcga ggaggccgcg
    24661 gtcacggtcg ccttcctcac cctggcattc gcccagctgt ggcacgtgtt caacatgcgc
    24721 gaggccggca gcccgctctt cgccaacgag atcacgcgca acggctacgt ctggggcgcg
    24781 ctcgcgctgt gcgtcgggct gatggcggtc gcggtcgggg tgccgccggt ggccgaggtg
    24841 ctggggctcg cggcgcccga tctgcgcggc tggctgctcg cactcgcctt gagcctggtg
    24901 ccgttggcgg cgggtcagct gctcaagctt gcgccgcggc gccggcgccg ctgaccgggt
    24961 cgcgaaccga gtcaggctcc tcggcgccgt cgaggtcgag gttgagagtg ccgccgcctg
    25021 cgaggcggtg gcgctggtcc ttgaagccga ccgtgatcgg ctccgccgcg cactcgaccg
    25081 cggcgatgcg cagccgtcgg tgggtgatct cgagggccag cgcatggccc cgatacctga
    25141 tccggaacct gaggcgcgtg agcgtgctcg gcaggcgcgg gttgaagtgc agcacgcccg
    25201 cgcgcggctc gatccccgaa tagcagcgct ggaccaggtc gacggtgccg gccatggcgc
    25261 ccagatgcac gccctcggcc gtggtgccac cctggatgtc ctcgatgtcg ctgttcagcg
    25321 cctcgtggaa caggcgccac gaccccggcc ggtcccagcg cgccagcacc caggaatgca
    25381 ccacccggct caaggtcgag ccgtcggagg tgcgctggag gtagtattcg atgttacgcg
    25441 ggatcgtttc gtagacgaat ggatagccct gccgctcgaa cagctcgcgc aactcctcgg
    25501 acgagaacag gtagaacagc atcagcacgt cggcctgctt cgacgccttg tagcggttgg
    25561 cgctgtcgtt ctcagcctcg aggatgcggt cgaggcgctg gatgttgccg tagcgctgcc
    25621 ggtagccgtc ccagtcgaac tcggccaggt cctcgtagcc cgcgaactgg ctgatgatgc
    25681 cgtcgtcgtg gaacggcacg aacatgttgc ggctgatcgc gtcccaggtc tcgaactcgt
    25741 ccgcgctcag gccgagcttg tcgcacaggc gccgctgctg gtcggcgggc aggatgtccc
    25801 tgagctccag cgcccggcac agcacccacg ccgccatgac gttggtatag gcgttgtcgt
    25861 cgaggcccgc gcgctccgcg tccggatagg cctcgtggta ctcgtccggg cccatcacgc
    25921 cgcggatgtg gtagcggccc gactcctcgt ccaggcgcgc gatcgaggtc cagaagcggg
    25981 cgatctcgta gatcacctcg gcgccgtaga aggccaggaa ctcgacgtcg cccgtgacct
    26041 ggaggtactg ccagatgttg taggcgatcg cggcgttgac gtggcgctgc agcgcactgt
    26101 tgtccgcggt ccatcgcccc gacttcgggt tgaggtggtg gagctggctg gtctcgcggc
    26161 cgtcgctggc gctctgccag ggaaacatgg cgccggcgaa gcccgcctcg cgcgccgcct
    26221 cgcgcgcctc gcccaagcgc cggtagcggt acatcatcag cgcgcgcgtg atctccggca
    26281 tgcggagcgt cagcgtcgga aagatgaaca gctcatccca gaacacgtgg ccgcgatagg
    26341 cctcgccggt caggccccgc gccggcaccc cggcgtcgag cccgatggtg ttgagggacg
    26401 cggtctggag cagatggaac aggtgcagcc tcagcgtcgc caccatccgg ttgctcgggc
    26461 tgccgtcgac gtcgacatcg attccgaact gctcccacaa atagcgccag gtgcgcgcgt
    26521 gcgcgccgag caggcg
//
LOCUS       DASWHU010000046         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_387313, whole genome shotgun sequence.
ACCESSION   DASWHU010000046 DASWHU010000000
VERSION     DASWHU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387313"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /gene="atpA"
                     /locus_tag="VGB88_01830"
     CDS             <1..1282
                     /gene="atpA"
                     /locus_tag="VGB88_01830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9646206.1"
                     /translation="EGDTVKRTGAIVDVPVGKALLGRVVDALGNPIDGKGPIATSERR
                     RVDVKAPGIIPRRGVHEPMQTGLKAIDSLVPIGRGQRELIIGDRQTGKTAVAVDTIIN
                     QRAVNQSGDESKKLYCVYVAIGQKRSTVAQIVKTLEDNGALDYSIVVSATASEPAPLQ
                     FLAPYSGCAMGEYFRDNGMHALIIYDDLSKQAVSYRQMSLLLRRPPGREAYPGDVFYL
                     HSRLLERAAKMNADNGAGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETELFYQ
                     GIRPAINVGLSVSRVGSAAQIKAMKQVAGRIKLELAQYREMAAFAQFGSDLDAATQRL
                     LNRGARLTELLKQPQYSPQPVEQQVVSIFAGVRGYLDGIDVAQVTRFEGMLLGEVRAK
                     HADLLKTIREQAELSEESEQRLHAIMKDFAKTFA"
     gene            1291..2184
                     /locus_tag="VGB88_01835"
     CDS             1291..2184
                     /locus_tag="VGB88_01835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX9646207.1"
                     /translation="MATLKSLKIRINSVRATQKITSAMKMVAASKLRRAQDAAVAARP
                     YAERMERMVSSLAASAADNESAPALLRGTGAEATHLLVVATSDRGLCGGFNSSIVRAV
                     RRRIALLADDGRAVKLFMVGRKGRDQLRREHGQRIVEAIEGVGQRGVNFAEAADIAGN
                     LSKRFDAGEFDVCTIYYNRFRSAVSQTVTAQQLIPFAPAAERRDGDDSGPAGLAVYEF
                     EPSEEAILANLLPRNMAVQIFHALLENGASEQGARMMAMDNATRNAGEMIDSLTLRFN
                     RTRQAAITKELIEIISGAEAL"
     gene            2218..>3140
                     /gene="atpD"
                     /locus_tag="VGB88_01840"
     CDS             2218..>3140
                     /gene="atpD"
                     /locus_tag="VGB88_01840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9646208.1"
                     /translation="MSNSVGRITQVIGAVVDVQFDGELPAILNAIETDNHGNRLVLEV
                     AQHLGESTVRTIAMDSTEGLVRGGEVSDTGGPITVPVGRETLGRIINVIGEPIDERGP
                     VETKRRAPIHAPAPAFTDQSTEAEILVTGIKVIDLLAPYAKGGKIGLFGGAGVGKTVI
                     IQELINNIAKAHGGVSVFAGVGERTREGNDLYHEMIESGVIDLDGAESKVALIYGQMN
                     EPPGARARVGLSGLTVAEYFRDQEGQDVLFFVDNIFRFTQAGSEVSALLGRIPSAVGY
                     QPTLSTDMGNLQERITTTRKGSITSVQAIYVP"
BASE COUNT          542 a         1084 c         1080 g          434 t
ORIGIN      
        1 cgagggcgac acggtcaagc gcaccggcgc catcgtcgac gtgccggtcg gcaaggcgct
       61 gctcggccgg gtggtcgatg cgctcggcaa cccgatcgac ggcaagggcc cgatcgcgac
      121 cagcgagcgc cgccgggtcg atgtcaaggc gcccggcatc atcccgcgcc ggggcgtgca
      181 cgagccgatg cagaccgggc tcaaggcgat cgattcgctg gtgccgatcg ggcgcggcca
      241 gcgcgagctg atcatcggcg accgccagac cggcaagacc gcggtggcgg tggacacgat
      301 catcaaccag cgcgcggtca accaaagcgg cgacgagtcg aagaagctct actgcgtcta
      361 cgtcgccatt ggccagaagc gctcgaccgt cgcccagatc gtcaagacgc tcgaggacaa
      421 cggcgcgctc gactactcga tcgtggtctc ggcgaccgcg tccgagccgg cgccgctgca
      481 gttcctcgcg ccctattcgg gctgcgcgat gggcgagtac ttccgcgaca acggcatgca
      541 cgcgctgatc atctacgacg acctgtcgaa gcaggcggtg tcctaccgcc agatgtcgct
      601 cttgctgcgc cgcccgcccg ggcgcgaggc ctatccgggc gacgtgttct atctgcactc
      661 gcgcctgctc gagcgcgcgg ccaagatgaa cgccgacaac ggcgccggct cgttgaccgc
      721 gctgccgatc atcgagaccc aggcgggcga cgtgtccgcc tacatcccga ccaacgtcat
      781 ctcgatcacc gacggccaga tcttcctcga gaccgagctg ttctaccagg gcatccgccc
      841 ggcgattaac gtcggcctgt cggtgagccg ggtcggctcg gcggcgcaga tcaaggccat
      901 gaagcaggtt gccgggcgga tcaagctcga gctcgcccag taccgcgaga tggcggcgtt
      961 cgcccagttc ggctccgacc tcgacgccgc cacccagcgc ctgctcaacc gcggcgcgcg
     1021 cctgaccgag ctgttgaagc agccgcagta cagcccgcag ccggtcgagc agcaggtggt
     1081 gtcgatcttc gccggcgtgc gcggctatct ggacggcatc gacgtcgccc aggtgacccg
     1141 gttcgagggc atgctgctgg gcgaggtgcg cgccaagcac gccgacctgc tgaagacgat
     1201 ccgcgagcag gccgagctca gcgaggagag cgagcagcgg ctgcacgcga tcatgaagga
     1261 cttcgccaag accttcgcgt aagcctgccg atggccaccc tcaaaagcct caagatccgg
     1321 atcaacagcg tccgggcgac gcagaagatc accagcgcga tgaagatggt cgcggcgtcc
     1381 aagctgcgcc gcgcgcagga cgcggccgtg gccgcgcgcc cctacgccga gcgcatggag
     1441 cggatggtgt cctcgctagc ggcgtcagcg gcggacaacg agtcggcgcc ggcgttgctg
     1501 cgcggcaccg gcgccgaggc cacgcatctg ttggtggtgg cgacctccga ccgcggcctg
     1561 tgcggcggct tcaacagctc gatcgtgcgc gcggtgcgcc ggcgcatcgc cctgctcgcc
     1621 gacgacgggc gtgcggtgaa gctgttcatg gtcgggcgca agggccgcga ccagcttcgc
     1681 cgcgagcacg gccagcgcat cgtcgaggcg atcgaggggg tcggccagcg cggcgtcaac
     1741 ttcgccgagg cggccgacat cgccggcaac ctgtcgaagc ggttcgatgc cggcgagttc
     1801 gacgtctgca ccatctatta caaccgcttc cgctccgcgg tctcgcagac ggtcaccgcg
     1861 cagcagctga tcccgttcgc ccccgcggcg gaacgccgcg acggcgacga cagcggaccg
     1921 gcgggcctgg cggtctacga gttcgagccc agcgaggaag ccatcctggc taatcttttg
     1981 ccgcgcaaca tggcggtgca gatcttccac gccctgctcg agaacggcgc cagcgagcag
     2041 ggtgcgcgca tgatggcgat ggacaacgcc acgcgcaacg ccggcgagat gatcgacagc
     2101 ctgaccttgc gcttcaaccg cacccgccag gcggcgatca ccaaggagct gatcgagatc
     2161 atctccggcg ccgaggcgct gtagaatgcg gcgcgcacgc gacatgggag caggagcatg
     2221 agcaacagcg tcggacgaat cacccaggtc atcggcgcgg tggtcgacgt gcagttcgac
     2281 ggcgagctgc cggcgatcct gaacgcgatc gagaccgaca accacggcaa ccggctggtg
     2341 ctcgaggtcg cccagcatct gggagagagc acggtgcgca ccatcgccat ggactcgacc
     2401 gagggcctgg tgcgcggcgg cgaggtgagc gacaccggcg gcccgatcac ggtgccggtc
     2461 gggcgcgaga cgctcgggcg gatcatcaac gtgatcggcg agccgatcga cgagcgcggc
     2521 ccggtcgaga ccaagaggcg ggcgccgatc cacgcgcccg cgcccgcctt caccgaccag
     2581 tcgaccgagg ccgagatcct ggtcaccggc atcaaggtga tcgacctgct cgcgccgtac
     2641 gccaagggcg gcaagatcgg cctgttcggc ggcgccggcg tcggcaagac cgtgatcatc
     2701 caggagctga tcaacaatat cgccaaggcc catggcggcg tctcggtgtt cgccggcgtc
     2761 ggcgagcgca cccgcgaggg caacgacctc tatcacgaga tgatcgagtc cggcgtcatc
     2821 gacctcgacg gggccgagtc caaggtggcg ctgatctacg gccagatgaa cgagccgccc
     2881 ggcgcgcgcg cgcgcgtcgg cctgtcgggg ctgaccgttg ccgagtactt ccgcgaccag
     2941 gaaggccagg acgtgctgtt cttcgtcgac aacatcttcc gcttcaccca ggcgggctcc
     3001 gaggtctcgg cgctgctggg ccgcatcccg agcgcggtcg gctaccagcc gacgctgtcg
     3061 accgacatgg gcaatttgca ggagcggatc accaccacgc gcaagggctc gatcacctcg
     3121 gtgcaggcga tctacgtgcc
//
LOCUS       DASWHU010000047         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1126854, whole genome shotgun sequence.
ACCESSION   DASWHU010000047 DASWHU010000000
VERSION     DASWHU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126854"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /gene="thrC"
                     /locus_tag="VGB88_01845"
     CDS             <1..997
                     /gene="thrC"
                     /locus_tag="VGB88_01845"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9646209.1"
                     /translation="VLGATSGDTGSAAIHAFRGRPNVDIFILHPEGRVSEVQRRQMTC
                     VADSNVHNIAVRGTFDDCQALVKAMFNDPSFRRAHRLSSVNSINWARVMAQAVYYCTA
                     AAKLGLPAQGVAFAVPTGNFGDVYAGYVAHRMGLGVWRLMVATNRNDILARALRRGDY
                     HLGPVHATISPSMDIQVSSNFERLLFELYDRDGAAVAGLMDGLARHGGFEIPQARLAR
                     LRALFDAASIGEDETLATIGRVHAECGRLIDPHTAVGVAAARRLAPPEGVPVVMLATA
                     HPAKFPDAVARATGRRPPPPPALAALMDASERFAVLDRDLGQIQDYIRAHAAAAA"
     gene            1021..>2231
                     /locus_tag="VGB88_01850"
     CDS             1021..>2231
                     /locus_tag="VGB88_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9646210.1"
                     /translation="MAKTRTSNGIDVRLTTLEGGLRVISQHMPGFATCSAGVWVGAGA
                     RNESATLNGISHLIEHMAFKGTERRDAYAIAAEIEAVGGHLNAYTSREHTAFYAKVLK
                     DDLPLALDILGDILCHSTFADDELAREKEVVIQEIGQAHDTPDDIVFDYFQETAYPEQ
                     SIGRPVLGSADGVSQLTRDQLFEYMASHYTAPDMVLAAAGHVDHERLVALVGEAFATL
                     PALSGPTFDKARYLGGEFRSDRALEQLHLLIGLDGFAYQDDDFYALQVLSTLLGGGMS
                     SRLFQEVREKRGLAYSVYSFAGSYVDGGLFGIYAGAAPEDAAELAGVLCDETRKVADG
                     VSDEEVARARAQLKSGLLMSLESSYTRCETLARQLLIYGRAKTIEEMVAEIDAVGPEH
                     VRACMTRLLGTA"
BASE COUNT          333 a          812 c          768 g          318 t
ORIGIN      
        1 cgtgctcggc gccacctcgg gcgacaccgg ctcggccgcg atccacgcct tccgcggccg
       61 gcccaacgtc gacatcttca tcctccaccc cgaaggaagg gtgtcggagg tccagcgccg
      121 gcagatgacc tgcgtcgccg actcgaacgt ccacaacatc gccgtgcgcg gcacgttcga
      181 cgactgccag gcgctggtca aggcgatgtt caacgatccg tccttccggc gcgcgcaccg
      241 cctgtcgtcg gtcaactcga tcaactgggc gcgggtgatg gcgcaggcgg tctactattg
      301 cacggcggcg gcgaagctcg gcctgcccgc gcagggcgtc gcgttcgcgg tgccgaccgg
      361 caacttcggc gatgtctacg ccggctacgt cgcgcaccgg atggggctcg gggtgtggcg
      421 gctgatggtc gcgaccaacc gcaacgacat cctggcccgc gcgctccggc gcggcgacta
      481 tcacctcggc ccggtgcatg cgaccatctc gccgagcatg gacatccagg tgtcgagcaa
      541 cttcgagcgc ctgctgttcg agctctacga ccgcgacggc gccgcggtcg caggcctgat
      601 ggacgggctc gcgcggcacg gcggcttcga aattccgcag gcgcggctcg cccggctgcg
      661 ggcactgttc gacgccgcca gcatcggcga ggacgagacc ttggccacca tcgggcgcgt
      721 ccacgccgag tgcgggcggc tgatcgaccc gcacaccgcg gtcggggtcg ccgccgcccg
      781 ccggctcgcg ccgcccgagg gggtgccggt ggtgatgctg gcgaccgcgc acccggccaa
      841 gttcccggac gcggtcgcgc gcgcgaccgg ccgccgcccg ccgccgccgc cggcgctcgc
      901 cgccctgatg gacgcatccg agcgctttgc cgtgctcgac cgtgacctcg gccaaatcca
      961 ggactatatt cgcgctcacg ccgccgccgc ggcatgaacg gcaacaggtc cggatccggc
     1021 atggccaaga ctcgcacatc gaacggtatc gacgtccgcc tgaccacgct cgagggcggc
     1081 ctcagggtga tcagccagca catgcccggc ttcgccacct gctccgccgg cgtgtgggtg
     1141 ggggccggcg cgcgcaacga gagcgcgacg ctcaacggca tatcgcacct gatcgagcac
     1201 atggcgttca agggcaccga gcggcgcgac gcctatgcca tcgcggccga gatcgaggcc
     1261 gtcggcgggc acctcaacgc ctacacctcg cgcgagcaca ccgcgttcta cgccaaggtg
     1321 ctcaaggacg acctgccgct cgcgctcgac atcctgggcg acatcctgtg ccactcgacg
     1381 ttcgcggacg acgagctcgc gcgcgagaag gaggtggtga tccaggagat cggccaggcc
     1441 cacgacaccc ccgacgacat cgtcttcgac tacttccagg agaccgccta tcccgagcag
     1501 tcgatcggcc ggccggtgct cggctcggcg gacggggtct cccagctgac ccgcgaccag
     1561 ctattcgaat acatggcgag ccattacacc gcgcccgaca tggtgctggc ggcggcgggc
     1621 cacgtcgacc acgagcgcct ggtggcgctg gtcggcgagg cgttcgcgac cctgcccgcg
     1681 ctcagcggcc cgacgttcga caaggcgcgc taccttggcg gcgagttccg ctccgaccgc
     1741 gcgctcgagc agctccactt gctgatcggc ctggacggat tcgcctacca ggacgacgac
     1801 ttctatgccc tgcaggtgct gtcgaccctg ctcggcggcg gcatgtcgtc gcggctgttc
     1861 caggaggtgc gcgagaagcg cgggctggcc tactcggtgt acagcttcgc cggcagctac
     1921 gtcgacggcg gcctgttcgg gatttacgcc ggcgccgcgc ccgaggacgc ggccgagctc
     1981 gccggcgtgc tgtgcgacga gacgcgcaag gtcgccgacg gcgtcagcga cgaagaggtc
     2041 gcgcgcgccc gcgcacagct caagtcgggc ctcttgatgt cgctcgagag cagctacacg
     2101 cgctgcgaga cgctggcccg ccagctgctg atctacggcc gggcgaagac gatcgaggag
     2161 atggtggccg agatcgacgc ggtcgggcct gagcacgtgc gcgcgtgcat gacgcgcctg
     2221 ctcggcaccg c
//
LOCUS       DASWHU010000048         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3343243, whole genome shotgun sequence.
ACCESSION   DASWHU010000048 DASWHU010000000
VERSION     DASWHU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343243"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VGB88_01855"
     CDS             <1..1587
                     /locus_tag="VGB88_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEX9646211.1"
                     /translation="KFHGVYQQVDRDTATALKQDGRAKTHRLMVRMKIPGGCLTPEQY
                     LVADDLAGACADGSLRITTRQDFQLHGVIKGDLAPTVAEINTALITTFGACGDVVRNV
                     VTTPAPVADAVHRRLRDDAVRLSNHFLPATRAYHEIWLDGSPLPESAGAETEPLYGAT
                     YLPRKFKIGLATPDDNSADVLTNDLAVIAFFEGERLLGYDFALGGGLGMTHNKPETYP
                     RLATPIAFVEPDGLLTAAEAVVCLQREHGDRSNRRHARLKYLVAEKGAEWVKAALERI
                     LGRPLAPPLPLPRLSVPDHLGWHPQGDGKWWLGVPVASGRIADRGGVRLRSALREIVT
                     EHAPGVVLTPDQNILLAGLPRGARRRIEAALGRHGVALREHISEVERWALACPALPTC
                     GLALAEAERVRAPLVAAIEAALARHGLGEERLSVRITGCPNGCARPYAGDIGIVGRMP
                     GHYAVYVGGDFAGTRLSFRLLDKVPLAGVATALEPLFALFAARRRPGEGFGDVCHRLG
                     AEALGQAIGGAAGTLVREVA"
     gene            1587..2327
                     /locus_tag="VGB88_01860"
     CDS             1587..2327
                     /locus_tag="VGB88_01860"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9646212.1"
                     /translation="MEIAGGGAAAVEQAHALARAHGHLGGAALLRVMIERVFAGRIAV
                     VTSFGAESAVLLHMVAEVDPATPVIFLDTLKHFPETLAYRDALAARLELCDVRTVRPD
                     ARYLAGPDPDGELWRADPDLCCRLRKVLPLERALAGFAAWVTGRKRYQGGVRGSLAPI
                     EAVDGRIKINPLARWPRAAVRAALARHRLPRHPLAGRDLISIGCAPCTALVARGEEPR
                     AGRWQGTDKTECGIHLPLPAQASRTRAP"
     gene            2455..3606
                     /locus_tag="VGB88_01865"
     CDS             2455..3606
                     /locus_tag="VGB88_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9646213.1"
                     /translation="MLSLASALALAAAPGRADMMLAGVDNKVEFNDQGPVFVAPGNDL
                     VSVIDIGTDPEAPRIVANLPLINSVFGPPTNLAITPDGRLALVANAMNWIADGDGWKP
                     DPDNRIHVIDLTLDPPQAIGEVEVGRQPSGLAISRQGDLALVAHRADNSVGVLAIEDK
                     TVRLVQIVDMGEQVAAVAIAPDGRRALVAKFPGHKIAVLDIAGGRATYDQKQDMPVGL
                     WPYNVKISPDGGLALTADNGNAGQSDGHVDTVSVIDLEASPPRVIDRIAVGDAPEGLA
                     ISPTGRVAVAVLLKGSAGIPPDRWYYHRNGTVVVLAIDGKSVRRVAEVEVRGLPEGVV
                     FSADGKYLYVGNFVDGDISILAVDGTTVTNTGKTLALPGHPGSMGASTP"
     gene            complement(4008..>4322)
                     /locus_tag="VGB88_01870"
     CDS             complement(4008..>4322)
                     /locus_tag="VGB88_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646214.1"
                     /translation="VSAGGQSFPIGVETVVQMFEPRLLRMIEMDHYQALQDFLAPFVA
                     RYDDLAKIKQQALANIPRFLGAAESAALKGDIASASNVFEALETYLSYVGRKQLKHIF
                     PV"
BASE COUNT          609 a         1569 c         1517 g          627 t
ORIGIN      
        1 aagttccacg gcgtctacca gcaggtcgac cgcgacaccg cgaccgcgct caagcaggac
       61 ggccgcgcca agacccaccg gctgatggtg cgcatgaaga tccccggcgg ctgcctgacg
      121 ccggagcaat atctggtcgc cgacgacctg gccggagcct gcgccgacgg cagcttgcgc
      181 atcaccaccc gccaggactt ccagctgcac ggcgtgatca agggcgatct ggcgccgacc
      241 gtcgccgaga tcaacaccgc gctgattacc accttcggcg cgtgcggcga cgtcgtgcgc
      301 aacgtcgtca ccacgccggc gccggtcgcc gacgcggtgc accgccggct gcgcgacgac
      361 gcggtgcggc tgtccaacca cttcctgccc gcgacccggg cctaccacga gatctggctc
      421 gacggcagcc cgctgcccga atccgccggc gcggaaaccg agccgctcta cggcgcgacc
      481 tatctgccgc gcaagttcaa gatcggcctc gccacgccgg acgacaacag cgccgacgtc
      541 ctcaccaacg atctcgccgt catcgccttc ttcgaaggcg aacggttgct cggctacgat
      601 tttgcgctcg gtggcggtct tgggatgacc cacaacaagc ccgagaccta tccgcgcctc
      661 gcgaccccga tcgcgttcgt cgagcccgac ggcctgctca ccgccgcaga ggccgtggtg
      721 tgcctgcagc gcgagcacgg cgaccgctcg aaccgcaggc acgcgcggct gaagtacctg
      781 gtcgcggaga agggggcgga gtgggtcaag gccgcgctcg agcgaattct gggacgaccg
      841 ttggcgccgc cgctgccgct gccgcgcctc tcggtccccg accatctggg ctggcatccc
      901 cagggcgacg gcaagtggtg gctcggcgtg ccggtggcga gcgggcggat cgccgaccgc
      961 ggcggggtgc ggctgcgctc cgcgctccgc gagatcgtca ccgaacatgc gcccggggtc
     1021 gtcttgactc ccgaccagaa catcctgctc gccggcctgc cgcgcggcgc gcgtcggcgc
     1081 atcgaggcgg cgctcggccg ccacggcgtc gccttgcgcg agcacatctc ggaggtcgag
     1141 cgttgggcgc tcgcctgtcc ggcgctgccg acctgcggcc tggcgctggc cgaggccgag
     1201 cgggtgcgcg caccgctggt cgcggcgatc gaggcggcgc ttgcccgcca cggtctcggc
     1261 gaggagcgcc tcagcgtccg catcaccggc tgccccaacg gctgcgcgcg gccctatgcc
     1321 ggcgacatcg gcatcgtcgg gcgcatgccg ggccattacg cggtctatgt cggcggcgat
     1381 ttcgccggca cgcggttgag cttccgcctg ctcgacaagg tgccgttggc gggggtggca
     1441 accgcgctcg agccgctgtt cgcgctgttc gccgcccgcc gccggccggg cgaggggttc
     1501 ggcgacgtct gccaccgcct cggcgccgag gctctcggcc aggcgatcgg cggggcggcc
     1561 ggcacactgg tccgggaggt ggcgtgatgg agatcgccgg cggcggcgcg gcggcggtcg
     1621 agcaggcgca tgcgctcgcg cgcgcccacg gccatctggg cggcgccgcg ctgctgcggg
     1681 tgatgatcga gcgggtgttt gccggccgga tcgcggtggt gacctcgttc ggcgccgagt
     1741 ccgcggtgct gctgcacatg gtcgccgagg tcgatccggc gacgccggtg atctttctcg
     1801 acacgctcaa gcatttcccg gaaacgctcg cctatcgcga cgcgctggcc gcgcgcctcg
     1861 agctttgcga cgttcgcacc gtgcggcccg acgcgcgcta cctcgccggc ccggaccccg
     1921 acggcgagct gtggcgcgcc gaccccgacc tctgctgccg cctgcgcaag gtgctgccgc
     1981 tcgagcgcgc gctcgccggg ttcgccgcct gggtgaccgg gcgcaagcgt taccagggcg
     2041 gcgtgcgggg cagtcttgcg ccgatcgagg cggtcgacgg gcggatcaag atcaacccgc
     2101 tcgcccgctg gccgcgggcc gcggtgcgcg cggcgctcgc ccgccaccgc ctgccgcgcc
     2161 acccgctcgc cggccgcgac ctgatctcga tcggctgcgc gccgtgcacg gcgctggtgg
     2221 cgcggggcga ggagccgcgc gccggccgct ggcaggggac cgacaagacc gagtgcggca
     2281 ttcacctgcc gctgcccgcg caggcgtcgc gcacgcgcgc tccgtgatcg cccgggccgg
     2341 tcccggcttg cgcccgccgg cgcgcctggg gcatgcttct cgcggcggcg ccgtcgcgcg
     2401 ccccgagggg aggacaggga catgctgcac gatcggccgg cacggcgatg ggccgtgctc
     2461 tcgttggcgt ccgcgctggc gctcgccgcc gctccgggac gcgccgacat gatgctcgcc
     2521 ggggtcgaca acaaggtcga gttcaatgac caggggccgg tgttcgtcgc cccgggcaac
     2581 gacctcgtct cggtgatcga catcggtacc gatcccgagg cgccgcgcat cgtcgccaac
     2641 ctgccgttga tcaactcggt gttcgggccg ccgaccaacc tcgccatcac ccccgacggg
     2701 cgactggctc tggtcgccaa cgcgatgaat tggatcgccg acggcgacgg ctggaagccc
     2761 gacccggaca accgcattca cgtcatcgac ctgaccctcg atccgccgca ggcgatcggc
     2821 gaggtcgagg tcggccgcca gccctcgggc ctggcgatca gccgccaggg cgacctggcg
     2881 ctggtcgcgc accgcgccga caactcggtc ggcgtgctcg cgattgagga caagaccgtg
     2941 cgcctggtcc agatcgtcga catgggcgag caggtcgccg cggtcgcgat cgcgcccgac
     3001 ggccgccgcg cgctggtcgc caagttcccg ggacacaaga tcgcggtgct cgacatcgcg
     3061 ggcgggcgcg cgacctacga ccagaagcaa gacatgccgg tcgggctgtg gccgtacaac
     3121 gtcaagatca gccccgacgg cggcctcgcg ctcaccgccg acaacggcaa cgccggccag
     3181 tccgacggcc acgtcgatac ggtcagcgtc atcgaccttg aggcgagccc gccgcgggtg
     3241 atcgaccgca tcgcggtcgg cgacgcgccc gagggcctcg ccatcagccc gaccgggcgg
     3301 gtggcggtgg cggtgctgct caaaggcagc gccggcatcc cgccggaccg ctggtactac
     3361 caccgcaacg gcaccgtggt ggtgctcgcg atcgacggca agtcggtgcg ccgcgtggcc
     3421 gaggtcgagg tccgcggcct gcccgaaggc gtcgtgttca gcgccgacgg caagtacctc
     3481 tatgtcggca actttgtcga cggcgacatc tcgatcctcg cggtcgacgg caccacggtc
     3541 accaacaccg gcaagactct cgcgctcccc ggccaccccg gctcgatggg cgccagcacg
     3601 ccgtgacggc gttccaaccc ggatcgcaag ggtgatcgcc aaggagagaa atgcagcgat
     3661 cgtccatagt ggtcctcgcg ctatgcctgc cgttggttcg cgaggccgat gccggagagc
     3721 tggcgccggg caatagcggc gcctcggata ccgcgacagt catccagttg atggacccgc
     3781 acccggtcgg cagcgggctt acagtggcgc aggcttgttg caaggtctgc cgaaagggca
     3841 gggcctgcgg cgactcctgc atcgcccgcg gcaagacctg caccaggccg ccggggtgcg
     3901 cctgtgacgc gcaatgaacc gtggcggtcg gctcggttac ttcaccactt ccttgcgcgg
     3961 ccggccggtg tcgtgagccg tcagcaagcc gtaacggtct cggttggtca cactggaaag
     4021 atgtgcttga gctgcttgcg gccgacgtag ctcaggtagg tctcgagcgc ctcgaataca
     4081 ttgctggcgc tggcgatatc acccttcagc gccgcgcttt ccgcggcacc caaaaatctt
     4141 gggatattgg cgagcgcctg ctgcttgatc tttgccaagt catcgtatcg cgcgacgaac
     4201 ggggccagga aatcctgcag tgcctggtag tggtccatct cgatcatgcg cagcagacgt
     4261 ggctcgaaca tctggaccac cgtctcgacg ccgatcggga agctctgacc gccggccgat
     4321 ac
//
LOCUS       DASWHU010000049         4617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1867493, whole genome shotgun sequence.
ACCESSION   DASWHU010000049 DASWHU010000000
VERSION     DASWHU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867493"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..2114)
                     /gene="asnB"
                     /locus_tag="VGB88_01875"
     CDS             complement(246..2114)
                     /gene="asnB"
                     /locus_tag="VGB88_01875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX9646215.1"
                     /translation="MCGIAGFTNPAADAPAILDDMLEAMAWRGPDGRGVHVDGDLGLG
                     HVRLAVIDLDGGAQPRVDQASGDALVFNGEIYGYRAHAAALHQAGVALADDSDTEVLF
                     QMLRRHGVAETLRRIDGMFAFAFRDGAGGRLYLARDRFGEKPLFYGVRDGALAFASEP
                     GALRRHPGYRSAGLDAHAVAQFLAFDFVPGPRSGFDGIAKLGAGSVLCFAGGTAEVER
                     FWRPAVEPGDGWTGDPLDRLETLLDRSVRERLIADVPVGVFLSGGIDSSLVAAVAARH
                     APAITAYTVSFADQSYDEAPFAAEVARHLGLRHEVAELATGDLLAGLDAVEARISEPL
                     ADPSILPTWLVCRHARAGATVALGGDGADELFAGYPNFAVRRWAPLMAAMPRALGRLL
                     AAAVGALPASEGYMSLDFRLRQLAQGLGAPADLQSFLWMAPFAGRDHAALFSDAFPAR
                     AADPLAPAAEAVAALDVRGGLARLQRLFLRFYLAEDILAKVDRASMFHSLEVRSPFLD
                     RDLAAFALSLPARLKLGAGGGKHLLRRLARKLLPARVAERRKHGFAPPIAALMRGPLR
                     ARVTDVVLDPGNALAPWFDRGTLEALVTRHMSGRFDHRKQLWALYVLFSWARRAAA"
     gene            complement(2123..2851)
                     /locus_tag="VGB88_01880"
     CDS             complement(2123..2851)
                     /locus_tag="VGB88_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9646216.1"
                     /translation="MDPSHYRLDEELAERHWWWVARRNILASLVAHAIAPAGDARQIL
                     EVGCSTGSNLALLGRFGTVTGLEMHAPTAERCRARHPGTEVLCAAIPAPLARRFDAIC
                     AFDVLEHIGDERGAVDWLADHLKRPGQLFVAVPAFAFLWSRHDNLAHHRRRYTRAGLA
                     RALAPRFDVTYATYFNFHLFPAIAAVRLAQRALGVGSGRDDKRVGGGWANGLLRAVFD
                     AERLWLPRVPLPVGVSLFMAATAR"
     gene            complement(2859..3806)
                     /locus_tag="VGB88_01885"
     CDS             complement(2859..3806)
                     /locus_tag="VGB88_01885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9646217.1"
                     /translation="MGRSLSIVVPVYNEAGNVEELYRQIASVVAEQGYDAEIVLVDDA
                     STDGSAEVLDRLARAHGEAKVIHLARNYGQTAALMAGLDHSTAAVVVAIDGDLQNDPA
                     DIPRLLAKLEHGFDVVSGWRRARREGLARTLPSRLANWLIARTTGVRLHDFGCTLKAY
                     RRDVLGDVRLYGEMHRFIPVYAAWAGGRIAELEVSHRPRRAGASKYGLGRTGRVLLDL
                     VVVLFLHRALDRPVQFFGRIGLWSFGLGILAGAYALYLKVVEDVSFILTPLPLLISLL
                     WLTGLLCVLLGVVAEMLTRIYFESRNSPFYRIRSTLNLE"
     gene            complement(3875..>4617)
                     /locus_tag="VGB88_01890"
     CDS             complement(3875..>4617)
                     /locus_tag="VGB88_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646218.1"
                     /translation="AACPGLRTIVFMGTGASSYIDLDAARRRGIRVHTISGYGDRSVA
                     EHALALMLAGGRKIATMDRAIRAGEWATLDGVELAGKTLGVVGTGGIGKEMVRLGAAL
                     AMRVIAWNRSGVPADLPAEACALDELLASADVISLHLALNDQTREIIDRRRIGLIRPQ
                     AMLVNTARGGLVDEPALVEALQAGRLAHAALDVFADEPLAAGHPLARLDNVTLTAHAG
                     FMTREASERLLRRAFEIVARESAAPPRP"
BASE COUNT          718 a         1663 c         1673 g          563 t
ORIGIN      
        1 acgggctcac gtccggccat tgctcgcgcg cgccgtcgag cacgtactga aagctgtccc
       61 ggcatcccgc catgccttgc gcacgagctg cgagcggcgc cgccgacatg acgagcgcga
      121 ggagactccc gatcagccta cagttcatga tcgtcccgtc gtcgtctgca tcggcgcggc
      181 gcacgagcgt tgcgagtctg tgccacggcc ggtgaaaaca caagcgcgcc cggttgtccc
      241 ggttctcagg ccgcggcgcg gcgcgcccag ctgaacaaca cgtacaacgc ccatagctgc
      301 ttgcggtggt cgaagcgtcc gctcatgtgg cgtgtaacca gcgcctcgag cgtcccgcgg
      361 tcgaaccacg gcgccagcgc attgccggga tcgagcacca cgtcggtgac gcgcgcgcgc
      421 aacggcccgc gcatgagggc cgcgatcggc ggcgcgaagc cgtgctttcg ccgctcggcc
      481 acgcgcgccg gcagcagctt gcgcgcaagc cgcctgagca ggtgcttgcc gccgccggcg
      541 ccgagcttca agcgcgccgg cagcgacagc gcgaatgccg caaggtcgcg gtcgagaaac
      601 gggctgcgca cctcgagact gtggaacatc gacgcccgat cgaccttggc caggatgtcc
      661 tcggccagat agaagcgcag gaacaggcgc tgcaggcgcg cgagcccgcc gcggacgtcg
      721 agcgcggcca ccgcctcggc ggccggcgcc agcgggtcag cggcgcgcgc cggaaaggcg
      781 tcggagaaca gcgccgcgtg gtcgcggccg gcgaacggcg ccatccacag gaacgattgc
      841 aggtcggcgg gcgcgccgag accttgggcg agctggcgca gcctgaagtc gaggctcata
      901 tagccctcgg acgcgggcag cgcgccgacc gcggcggcca gcaggcggcc gagcgcgcgc
      961 ggcatcgccg ccatcagcgg cgcccaccgc cggaccgcga agttcggata gccggcgaac
     1021 aattcgtcgg cgccgtcgcc gccgagcgcg accgtggcgc cggcgcgggc gtgccggcag
     1081 accagccagg taggcaggat cgagggatcg gcgagcggct cgctgatccg cgcctcgacc
     1141 gcatcgagcc ctgcgagcag gtcaccggtg gcgagctcgg cgacctcgtg cctgagcccg
     1201 agatggcgcg ccacttcggc cgcgaacggc gcctcgtcgt agctctggtc ggcgaagctg
     1261 acggtgtagg cagtgatcgc gggcgcgtgg cgggcggcga ccgccgcgac caggctcgag
     1321 tcgatgccgc cggacaggaa cacgccgacc ggaacgtcgg cgatcagccg ctcgcgcacc
     1381 gaccggtcga gcagcgtctc gagccggtcg agcgggtccc ccgtccaacc atcgccgggc
     1441 tccaccgccg gccgccagaa ccgctcgacc tcggcggttc cgcccgcgaa gcacagcacc
     1501 gagccggcgc cgagcttggc gatgccgtcg aagcccgagc gcgggccggg gacgaagtcg
     1561 aaagcaagga actgcgccac cgcgtgcgca tcgaggcccg cgctccgata ccccgggtgg
     1621 cggcgcagcg cgcccggctc ggaggcgaag gcgagcgcgc cgtcgcgcac gccgtagaac
     1681 agcggcttct cgccgaagcg gtcgcgggcg agatacaggc ggccgccggc gccgtctcga
     1741 aaggcgaaag cgaacatgcc gtcgatccgc ctgagcgtct cggcgacgcc gtggcggcgc
     1801 agcatctgga acaggacctc ggtgtcggag tcgtcggcaa gcgcgacgcc ggcctggtgc
     1861 aaggcggcgg cgtgcgcgcg atagccgtag atctcgccgt tgaacaccag cgcgtcgccg
     1921 ctcgcttggt cgacgcgcgg ttgggcgccg ccgtcgaggt cgatcaccgc aaggcgcaca
     1981 tgcccgaggc cgagatcgcc atcgacgtgg acgccgcgcc cgtccgggcc gcgccaggcc
     2041 atcgcctcca gcatgtcgtc gaggatcgcc ggcgcgtccg ccgcggggtt tgtgaacccg
     2101 gcaatgccgc acaccggcgc cgttacctcg cggttgcggc catgaacagc gacaccccga
     2161 ccggcagcgg cacgcgcggc agccacaggc gctcggcgtc gaagaccgcg cgcagcaggc
     2221 cgttcgccca gccgccgccg accctcttgt cgtcgcggcc gctgcccacg ccgagcgcgc
     2281 gctgggccag gcgcaccgcc gcgatcgccg ggaacaggtg gaagttgaag taggtcgcat
     2341 aggtgacgtc gaagcgcggc gccagcgcac gcgcgaggcc cgcgcgcgtg tagcggcggc
     2401 ggtggtgggc gaggttgtcg tgacgcgacc acaggaaggc gaacgccggc accgcgacga
     2461 acagctggcc gggccgcttc agatggtcgg cgagccagtc gaccgcgcct cgctcgtcgc
     2521 cgatgtgctc gagcacgtcg aacgcgcaga tggcgtcgaa ccggcgcgcg agcggcgccg
     2581 ggatcgccgc gcacagcacc tcggtccccg ggtggcgggc gcggcagcgc tcggcggtcg
     2641 gcgcgtgcat ctcaagcccg gtgacggtgc cgaaccgccc cagcagcgcc aagttggaac
     2701 cggtgctgca gccgacctcg aggatttggc gcgcatcgcc ggcgggggcg atcgcgtggg
     2761 cgacgaggct ggcgagaata tttcggcggg cgacccacca ccagtggcgc tcggccagct
     2821 cctcgtcgag ccgataatga ctcgggtcca ccgcgacgtc actccaggtt gagcgtgctg
     2881 cggatacgat agaagggcga gttgcgcgac tcgaaataga tccgggtcag catctcggcg
     2941 accacgccca acagcacgca caagagcccg gtcagccaca gcagcgagat cagcaacggc
     3001 agcggcgtca ggatgaacga gacgtcctcg accactttca ggtacagcgc ataggcgccg
     3061 gcgaggatgc cgaggccgaa gctccacaac ccgatccggc cgaagaactg caccggccgg
     3121 tcgagcgccc ggtgcaggaa cagcaccacc accaagtcca ggagcacgcg gccggtgcgc
     3181 cccagcccgt atttcgacgc acccgctcgg cgcgggcggt ggctgacctc gagctcggcg
     3241 atgcgcccgc cggcccaggc cgcatagacc gggatgaacc ggtgcatctc gccgtacagg
     3301 cgcacgtctc ccaggacgtc gcgccggtag gccttcagcg tgcaaccgaa gtcgtgcagg
     3361 cgcacgccgg tggtgcgcgc gatcagccag ttggcgagcc gcgacggcag ggttcgcgcc
     3421 aacccttcgc ggcgcgcccg ccgccagccc gagacgacgt cgaagccgtg ttcgagcttg
     3481 gcaagaaggc gcggaatgtc ggcgggatcg ttctgcaggt cgccgtcgat ggcgacgacg
     3541 accgcggcgg tgctgtggtc gagtcccgcc atcagcgccg cggtctggcc gtagttgcgc
     3601 gccaggtgga tgaccttggc ctcgccgtgc gcgcgcgcca gccggtcgag aacttccgcc
     3661 gagccgtcgg tgctggcgtc gtcgaccagc acgatctccg cgtcgtagcc ctgctcggcc
     3721 acgacactgg caatctgtcg atacagctcc tcgacgttgc cggcctcgtt gtagaccgga
     3781 accacgatcg acagcgaccg acccatgcct tcctccgcgc ccccgccggc aggcatcccc
     3841 cgccggcggc gagtatagca gcgcggccgg ggaatcaggg ccgaggcgga gccgcgcttt
     3901 cccgggcgac gatctcgaac gcgcggcgca gcaggcgctc cgacgcctcg cgggtcatga
     3961 acccggcgtg ggcggtgagc gtgacgttgt cgagccgggc caggggatgg cccgccgcca
     4021 gcggctcgtc ggcgaacacg tcgagcgccg cgtgggcgag ccggcccgcc tgtaatgcct
     4081 cgaccagggc tggctcgtcg accaggccgc cgcgcgccgt gttgaccagc atcgcctgcg
     4141 gccggatcaa gccgatccgg cgccggtcga tgatctcccg cgtctggtca ttaagcgcga
     4201 ggtggagcga gatcacgtcg gcgctcgcca gcagctcgtc gagcgcacag gcctcggccg
     4261 gcaggtccgc gggtaccccg ctccggttcc aggcgatcac gcgcatggcc agcgccgcgc
     4321 cgaggcgcac catctccttg ccgatgccgc cggtgccgac cacgcccagc gtcttgccgg
     4381 cgagctcgac gccgtcgagg gtggcccact cgccggcgcg aatcgcccgg tccatggtcg
     4441 cgatcttgcg cccgccggcg agcatcagcg ccagcgcgtg ctcggcgacg ctgcggtcgc
     4501 cgtagccgct gatcgtgtgc acccggatgc cgcggcgccg ggcggcgtcg aggtcgatat
     4561 agctggatgc gccggtgccc atgaacacga tggtccgcag gccgggacag gcggcga
//
LOCUS       DASWHU010000050        11069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_388703, whole genome shotgun sequence.
ACCESSION   DASWHU010000050 DASWHU010000000
VERSION     DASWHU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388703"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1325)
                     /gene="recF"
                     /locus_tag="VGB88_01895"
     CDS             complement(129..1325)
                     /gene="recF"
                     /locus_tag="VGB88_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX9646219.1"
                     /translation="MTTAAAAVLGPARDRIAAAAPPPSWVERLGVSEFRGYPRLDLHT
                     GPGPVVLTGANGAGKTNLLEALSLLAPGRGLRGARLGELARRGGDGRWAVSARLCTPG
                     GAVAVGTGLDPQGAGERRVVRIDGQGARGQAALAETCSVLWLTPAMDRLFSDAPAARR
                     RFLDRLVAGLEPGHGRELAAFERALRERARLLAERADGRWIDAVEETLAGHAVAVAAA
                     RREAVARIAGALAETRAPWPEARLALAGEVEGWLDEMPAVDAEQRLRARLGAGRRADA
                     EAGRAGCGPHRSDLAVTHLESGLPAAQCSTGEQKALLISIVLANARLHAARRGTTPLV
                     LLDEVAAHIDRVRRAALFEALAALGAQAWLSGTDAQLFEGLRGRAQFFVLADGRARAA
                     AGAARW"
     gene            complement(1322..2440)
                     /gene="dnaN"
                     /locus_tag="VGB88_01900"
     CDS             complement(1322..2440)
                     /gene="dnaN"
                     /locus_tag="VGB88_01900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9646220.1"
                     /translation="MRLTIERSHLLKSLQHVQSVVERRNTIPILSNLLISSADSQLSL
                     TATDMDIAVVDSSPSEVGQAGATTAPAHMLYDIVRKLPEGAQVEIATEDGNDRLSLRT
                     GRSKFSLHCLPTEDFPQVADGELGHQFALPAKDLRRLIDKSRFAISTEETRYYLNGIY
                     LHAVENGNQPVLRAVATDGHRLARVEVALPAGAEGMPGVIVPRKAIGELRKLLEDFDE
                     EVQVGLSEVKIRFVLGTATLSSKLIDGTFPDYARVIPEGNDKIMTVPGKAFADAVDRV
                     STIASDKSRAIKLSIADDKVVLSAHSPDQASATEELAVDYGGEAIDIGFNSRYVLDMT
                     QQIEGDSIKFEMADAASPTVVCDSDDPLALYVLMPMRV"
     gene            complement(2661..4049)
                     /gene="dnaA"
                     /locus_tag="VGB88_01905"
     CDS             complement(2661..4049)
                     /gene="dnaA"
                     /locus_tag="VGB88_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9646221.1"
                     /translation="MEGGLTNQRKECWQRVRGRLRDELGSTTFERWIESVELEAIERT
                     RIILSVPTRFLREWVAPRYGDRLRVLWQAELGGERTVEVVVRPGLAPELVDGADEDED
                     AAETRNDVHSLGEPSGDDVIGGRLDRRYSFENFVVGPPNEFAHAAARRVAESDNVPFN
                     PLFIYGGAGLGKTHLMHAIALRLRALRPERRVIYLSAEKFMYQFIRALRAKDVMGFKE
                     LYRSVDMLLIDDVQFIGGKDSTQEEFFHTFNALVDQNKQVVVSGDRSPADLEYMDERM
                     RSRLSWGLVAEVHPTSYELRLGILLAKGEQLGAREVPPKVLEFLAHKITSNVRELEGA
                     LTRVVAHSTLIGTPITLEGTQAVLHDLLRANDRRVSIEDIQKRVAEYYNIKVTEMSSA
                     RRAQMVARPRQVAMYLCKQLTQRSLPEIGRRFGNRDHTTVMHAVRKIEQLRQADTGFA
                     EDVELLRRMLES"
     gene            complement(4531..4797)
                     /gene="rpsT"
                     /locus_tag="VGB88_01910"
     CDS             complement(4531..4797)
                     /gene="rpsT"
                     /locus_tag="VGB88_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9646222.1"
                     /translation="MAYHKSAKKRVLRNEARRQVNRARVSRLRTFLKKVEAAIAGGDA
                     AAAQGALRAAQPEIMRGAGTGVIHKNTARRKLSRLSARIKALGG"
     gene            complement(4911..5684)
                     /locus_tag="VGB88_01915"
     CDS             complement(4911..5684)
                     /locus_tag="VGB88_01915"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9646223.1"
                     /translation="MVYESIKVEVRGKVGLITLDRPQALNALNAQLVEELNTALDAFE
                     SDPEIGAVLITGSDKAFAAGADIKEMQSKSYMDAYFGDFIGQWERIGSCRKPVIAAVA
                     GYALGGGCELAMMCDIVIAAEDARFGQPEIKLGTFPGSGGTQRLPRAIGKAKAMDLIL
                     TGRMMDADEAERAGLVSRVVPASELLARALEVAAGVAAMSLPAATMAKEAVNRAFESP
                     LREGVLFERRLFYSTFALDDQKEGMAAFVEKRPAKFTNR"
     gene            complement(5708..6112)
                     /locus_tag="VGB88_01920"
     CDS             complement(5708..6112)
                     /locus_tag="VGB88_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646224.1"
                     /translation="MATCNQPMLALRPIRRVAFALLCALAQPAAGAEAGEPAFIDGFD
                     DLPLMDGLEVIEGAGVLFDTPQGRIVERYATGALDAAEVGGFYLATLPALGWQPAGPL
                     RFVREGEALSITLYPPEASGRITVRFSLAPGA"
     gene            complement(6115..6945)
                     /gene="mutM"
                     /locus_tag="VGB88_01925"
     CDS             complement(6115..6945)
                     /gene="mutM"
                     /locus_tag="VGB88_01925"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX9646225.1"
                     /translation="MPELPEIETIRRGLAPRLIGRRITAVTVRRGDLRRPLARDFARR
                     LAGRAVIEVARRAKYLLIGLDSGETLIAHLGMSGRLYLRPGGTCAAQPHEHVVFAIDD
                     GSEVVFNDARRFGLFDLAPSRALARHPLLRHLGPDPLGPDFDAEVLSAALCGRRAPIK
                     ALLLDQRLVAGIGNIYACESLYRAGISPRRSGAGVAGVRAARLAAAIGAVLEEAIAAG
                     GSSLRDYVQANGELGYFQHNFSVYDRAGEACPRAARGHIVRRIVQSNRSTFYCPACQR
                     "
     gene            7019..7807
                     /locus_tag="VGB88_01930"
     CDS             7019..7807
                     /locus_tag="VGB88_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9646226.1"
                     /translation="MSARLGPKAPAAGPPETVPFGRLDVAPADKPALVARLFSGVAAR
                     YDLMNDLMSAGVHRLWRRALVAALELKPGAHVLDLAGGTGDIARRILEADDGARGVAV
                     TVCDLTPAMLAEGRRRAWDRGRASGLAWVCAEAARLPFAARSFDACTLAFGLRNMTRP
                     EPALAEVRRVLRPGARFLCLEFSPDVAPALAPVYDAVTGRVIPALGEIVAGDREAYQY
                     LVDSIRRFPTSARLAEMIAEAGFAGVRARAFAGGIAALHSAWRA"
     gene            7829..9352
                     /gene="ubiB"
                     /locus_tag="VGB88_01935"
     CDS             7829..9352
                     /gene="ubiB"
                     /locus_tag="VGB88_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX9646227.1"
                     /translation="MIRTARNLSRLAAIAVTLARHDALAPLEALGVAPWLVWLVRRLA
                     RRAAREARIGQRLAGAAVALGPGFIKLGQALSTRADLLGTEVAEDLAMLRDRLPPFPA
                     AEARAVVARELGVAVDEAFATFDDVPVAAASIAQVHFATTADGREVAVKVQRPGIEQA
                     FRRDLDLMVWLARLIERTSARWRRLRPVESVRTFAEMVAIEMDLSLEGAAASELGANF
                     ADDPSFNVPAVDWQRTARRVLTTDRVPGIAIDRREALIAAGHDPAELLAKAARATFLQ
                     VFRDGFFHGDPHPGNLFVDAAGNLWVVDFGIMGRLDIATRRFLGEMLTGFLNGDYEAV
                     ADVHFRAGFVPAGRSRGAFVQALRAIGEPILGLPANRISVGRLLGRLFQVTETFGMQT
                     QPHLLLLQKVMVVAEGVGRVLYPDANMWQLARPLIEDWMAAQSGLEARLGAAAEAVGE
                     IARRLPRIALQAERALDRLAAGPEPEAARARRADARIALAFALGLAAGALVALAALD"
     gene            9439..10665
                     /gene="coaBC"
                     /locus_tag="VGB88_01940"
     CDS             9439..10665
                     /gene="coaBC"
                     /locus_tag="VGB88_01940"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9646228.1"
                     /translation="MQPFEGKRILLIVAGGIAAYKSLELVRRLRERGAAVRTVLTAGG
                     AEFVTPLSLAALSEDKVYRDLFSLTDESEMGHIRLSREADLVVVAPASADLIAKMAAG
                     LADDLASTVLLATDTPVLIAPAMNHMMWQHPATRRNIATLARDGVRRIGPEAGDLACG
                     EVGAGRMAEPAAILAEIERLLAADADRPLHAVRALVTSGPTHEPIDPVRYLANRSSGK
                     QGHAIARAIGALGAEVTLVTGPTALADPACARVVRVETAQEMLAACEAALPVDVAVCA
                     AAVGDWRPARPANRKIKRDGGGPPALDLAENPDILATLAGRKRGRPTLVIGFAAETER
                     VVENATAKRAAKGCDWILANDVSAGTGVFGGDETTVHVIDSAGAETWAKASKDEVAER
                     LARRIAAAIGERGITA"
     gene            10662..>11069
                     /gene="dut"
                     /locus_tag="VGB88_01945"
     CDS             10662..>11069
                     /gene="dut"
                     /locus_tag="VGB88_01945"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX9646229.1"
                     /translation="MTAGAAVAVSVRRLDHALDGPLPGYETEHSAGVDLAAAVAEELV
                     IAPGGRALVPTGLALALPPGFEAQVRPRSGLALRHGITVLNSPGTIDADYRGEIGVVL
                     VNHGGEPFTVRRGMRVAQLVVAPVSRLAWQEVAL"
BASE COUNT         1471 a         4058 c         3977 g         1563 t
ORIGIN      
        1 gccgtcgtcg gtatcgccga tatacatgcc cgggcgcttg cgcaccgcgt ccaggccgcg
       61 cagcaccttg atcgactcgg cgccgtactc gccggcatcg gcgcgcggcg ggttctggat
      121 gtgcgtgctc accatcgcgc cgcacccgcg gccgcgcgcg cccggccgtc ggcgaggacg
      181 aagaactggg cgcggccccg caagccctcg aacagctggg catcggtgcc gctgagccac
      241 gcctgagcgc cgagcgccgc gagcgcctcg aacagcgccg cacgccgcac ccggtcgata
      301 tgggccgcga cctcgtccaa gagcaccagc ggcgtggtgc cgcgccgcgc cgcatgcagg
      361 cgcgcgttgg ccagcacgat ggagatcaag agcgccttct gctcgccggt cgagcactgc
      421 gccgcgggca gcccggactc gaggtgggtg acggcgaggt cgctgcgatg cgggccgcag
      481 ccggcacgcc cggcctcggc atcggcgcgc cggccggcgc caaggcgcgc gcgcaggcgc
      541 tgctcggcat cgaccgcggg catctcgtcg agccacccct cgacctcgcc ggcgagcgcg
      601 aggcgggcct cgggccacgg tgcccgcgtc tcggcgagcg cgccggcgat gcgcgccacc
      661 gcctctcggc gtgccgccgc caccgccacc gcgtgcccgg cgagggtctc ctcgaccgcg
      721 tcgatccagc gcccgtcggc gcgctcggcc aacagccgcg cccgctcgcg caaggcgcgc
      781 tcgaaagccg cgagctcgcg cccgtggccg ggctcgaggc cggcgaccag gcggtcgagg
      841 aagcgccggc gcgccgctgg cgcatcgctg aacaaccggt ccatggccgg ggtcagccac
      901 agcaccgagc aggtctcggc gagcgccgcc tggccgcgcg cgccctggcc gtcgatgcgc
      961 accacccgcc gctcgcccgc gccctgcggg tcgagcccgg tgccgactgc gaccgccccg
     1021 cccggggtgc agagccgcgc cgagaccgcc cagcggccgt cgccgccccg gcgcgcaagc
     1081 tcgccgagcc gcgcgccgcg caggccgcgg ccaggcgcga gcagggacag cgcctcgagc
     1141 aggttggtct tgccggcgcc gttggcgccg gtcagcacca ccggcccggg cccggtatgc
     1201 aggtcgaggc gcggatagcc gcggaactcg ctcacgccca gccgctcgac ccagctcggc
     1261 ggcggcgcgg ccgcggcgat gcggtcacgg gccgggccca ggacagccgc agcggccgtc
     1321 gtcatacgcg catcggcatc agcacgtaca gcgcgagcgg gtcgtcgctg tcgcagacca
     1381 ccgtcggcga ggcggcgtcg gccatctcga acttgatgct gtcgccctcg atctgctggg
     1441 tcatgtcgag gacgtaacgc gagttgaagc cgatatcgat cgcctcgccg ccatagtcga
     1501 ccgcgagctc ctcggtggcg ctggcctggt ccgggctgtg cgcggacagc accaccttgt
     1561 cgtcggcgat gctgagcttg atcgcccgcg acttgtcgct ggcgatggtc gagacccgat
     1621 cgaccgcgtc cgcgaacgcc ttgccgggca cggtcatgat cttgtcgttg ccctcgggga
     1681 tgacgcgcgc gtagtcgggg aaggtgccgt cgatcagctt cgacgacagg gtggcggtgc
     1741 cgagcacgaa ccggatcttg acctcggaca gcccgacctg gacctcctcg tcgaagtcct
     1801 ccagcagctt gcgcagctcg ccgatcgcct tgcgcggcac gatcacgccg ggcatgcctt
     1861 cggcacccgc cggcagcgcc acctcgaccc gcgccaggcg atggccgtcg gtcgccaccg
     1921 cgcgcagcac aggctggttg ccgttctcga ccgcgtgcag gtagatgccg ttcaggtagt
     1981 agcgcgtctc ctcggtcgag atcgcgaacc gcgacttgtc gatcagccgg cgcaggtcct
     2041 tggccgggag cgcgaactgg tggccgagct cgccgtccgc gacctgcggg aagtcctcgg
     2101 tcggcaggca gtggagcgag aacttggacc gcccggtgcg cagcgacagc cggtcgttgc
     2161 cgtcctcggt ggcgatctcg acctgggcgc cttcgggcag cttgcgcacg atgtcgtaga
     2221 gcatgtgcgc cggcgccgtg gtcgcgcccg cctggccgac ctcggacggg ctcgaatcca
     2281 ccaccgcgat gtccatgtcg gtcgcggtca gcgacagctg gctgtccgcg gacgagatca
     2341 gcaggttgga caggatcggg atggtgttgc ggcgctcgac cacgctctgg acatgctgga
     2401 gcgacttgag cagatgcgag cgctcgatgg tcagcctcat ggcgcggcgc gtcctcgtcg
     2461 aaaccgtcgt cgtgtgacgt gtattttgcc gccggcgcaa gggccgacgg gcccgccggc
     2521 ggcggcgagg cccccggggt ccgaactata gcagactcgg ccccggtcca caggattttg
     2581 tgcccgcgcc gcgcccccgg aggagccgcc caccaacgct tgggcggcgc ctgatcgggg
     2641 cgatttggcc ggtatcccgc ctaggactcg agcatgcgcc tgagcagctc gacgtcctcg
     2701 gcaaagcccg tgtcggcctg gcgcagctgc tcgatcttgc gcaccgcgtg catcaccgtg
     2761 gtgtggtcgc ggttgccgaa cctgcgcccg atctcgggca gcgagcgctg ggtgagctgc
     2821 ttgcacaggt acatggccac ctggcgcggc cgggcgacca tctgcgcgcg cctggccgag
     2881 ctcatctcgg tgaccttgat gttgtagtac tccgcgaccc gcttctggat gtcctcgatg
     2941 ctgacgcgcc ggtcgttggc gcgcaacagg tcgtggagca cggcctgggt gccctcgagc
     3001 gtgatcgggg tgccgatcag ggtcgagtgg gcgaccaccc gggtcagcgc gccctcgagc
     3061 tcgcgcacgt tcgaggtgat cttgtgggcc aggaactcga gcaccttggg cggcacctcg
     3121 cgcgcgccga gctgctcgcc cttggccagc aggatgccga ggcggagctc gtaggacgtg
     3181 ggatggacct cggccaccag cccccagctc aggcgcgagc gcatgcgctc gtccatgtat
     3241 tcgaggtcgg ccggcgagcg gtcgccggac accaccacct gcttgttctg gtcgaccagc
     3301 gcgttgaagg tgtggaagaa ctcctcctgg gtcgagtcct tgccgccgat gaactgcacg
     3361 tcgtcgatca gcagcatgtc gaccgagcgg tacagctcct tgaaccccat cacgtccttg
     3421 gccctgagcg cgcggatgaa ctggtacatg aacttttccg cggacaggta gatcacccgc
     3481 cgctcgggcc tgagcgcgcg caggcgcaac gcgatcgcgt gcatcaggtg ggtcttgccg
     3541 aggccggcgc cgccgtagat gaacagcggg ttgaacggca cgttgtcgga ctcggccacg
     3601 cgccgcgcgg cggcgtgcgc gaactcgttc ggcgggccga cgacgaagtt ctcgaagctg
     3661 tagcgccggt ccagccgccc gccgatcacg tcatcgcccg acggctcgcc caggctgtgc
     3721 acgtcgttgc gggtctcggc ggcgtcctcg tcctcgtcgg cgccgtcgac cagctcgggc
     3781 gcgagccccg gccgcaccac cacctcgacg gttcgctcgc cgcccagctc cgcctgccac
     3841 agcacgcgca gccggtcgcc atagcgcggc gccacccact cgcgcaggaa acgcgtcggc
     3901 acgctcaaga tgatgcgcgt ccgctcgatc gcctcgagct cgacggattc gatccagcgc
     3961 tcgaaggtgg tgctgcccag ctcgtcgcgc agccgtccgc gcacgcgctg ccaacactcc
     4021 ttccgctggt tcgtgaggcc cccttccacg gtcgcctggt cccccttcgg ccccgggcct
     4081 gcggctaagt ccgaggcgac ctcccgccgg ctattgcaac cgggcgggaa acatcatccc
     4141 agtcctgatc ggcctcacgg aaacttttaa gtccgtcccc tcgccgccgg gccgaataga
     4201 actgcaagct ctgcgcctgg aagtttccag attttacggg tctgtacgcc ggaacccttg
     4261 agccagtctc tcaagctcgc ggcgattgcc cgcttttttc gtctatccag acgaagaact
     4321 accgaggttc tgtttgcatg ccaataatcg gacagcgggg gcagagtacc ggcagcgacc
     4381 ggtccttgca acaacatcta catagtgagc gagcaaattt tttgcctgca tcggcgattc
     4441 tgcaacgccc gacattgacc acaggggctt agcggcaaac gtgcaatcgc gatggcagaa
     4501 aaataatcgc cgcgcgcgcg gcgcgaaggg tcagccgccg agcgccttga tccgtgccga
     4561 cagtcgcgac agcttgcgcc gcgcggtgtt cttgtgaatg acgccggtgc cggcgccgcg
     4621 catgatctcc ggctgcgccg cgcgcaacgc gccctgcgcc gccgccgcgt cgccgcccgc
     4681 gatcgcggcc tcgaccttct tgaggaacgt gcgcagccgg ctcacgcgcg cgcggttgac
     4741 ctgccgccgc gcctcgttgc gcagcacgcg cttttttgcc gacttgtgat acgccatggc
     4801 tcgatccaac cagatgcgcc agcgggcgcg caacgcccca tggcgggagc gcgcggttat
     4861 agccaacggt cccgccgccg tcaacaacgc cgccaagggc ggcgaatcgg tcagcggttg
     4921 gtgaacttcg ccggccgctt ctcaacgaag gcggccatgc cctccttctg gtcgtcgagc
     4981 gcgaaggtcg agtagaacag ccgccgctcg aacagcacgc cttcgcgcaa cgggctctcg
     5041 aaggcgcggt tgaccgcctc cttggccatg gtggccgcgg gcagcgacat cgcggcgacg
     5101 ccggccgcga cttcgagcgc gcgcgcgagc agctcggatg ccggcaccac gcggctgacc
     5161 agaccggcgc gctcggcctc gtccgcgtcc atcatccgcc cggtcaggat caggtccatt
     5221 gccttcgcct tgccgatcgc gcgcggcagg cgctgggtgc cgcccgagcc cgggaaggtg
     5281 ccgagcttga tctcgggctg gccgaagcgc gcgtcctcgg cggcaatcac gatgtcgcac
     5341 atcatcgcca actcgcagcc gccgcccagc gcgtagcccg cgaccgcggc gatcaccggc
     5401 ttgcggcagc tcccgatgcg ctcccactgg ccgatgaagt cgccgaaata ggcatccatg
     5461 tagctcttcg actgcatctc cttgatgtcg gcgccggcgg cgaacgcctt gtccgagccg
     5521 gtgatcagca ccgcgccgat ctcggggtcg gattcgaagg catcgagcgc ggtgttcagc
     5581 tcctcgacca gctgggcgtt gagcgcattg agcgcctgcg ggcggtcgag ggtgatcagg
     5641 cccaccttgc cccggacctc gaccttgatc gactcgtaga ccatgacgga cccccgtgtt
     5701 gctggcttca ggcgccgggc gcgagcgaga agcgcaccgt gatccgcccc gacgcctcgg
     5761 gcgggtagag ggtgatcgac agcgcctcgc cctcgcgcac gaagcggagc ggccccgccg
     5821 gctgccagcc gagcgcgggc aaggtcgcca ggtagaagcc gccgacctcg gccgcatcga
     5881 gggcgccggt cgcgtaacgc tcgacgatcc gcccttgcgg ggtgtcgaac aggacgcccg
     5941 cgccctcgat cacctcgagc ccgtccatca gcggcaggtc gtcgaagccg tcgatgaacg
     6001 ctggctcgcc ggcctctgcg cccgccgccg gctgggccag cgcgcacagc agggcgaacg
     6061 cgacacgtcg aatcgggcgg agcgcgagca tcggctggtt gcaggttgcc acgatcagcg
     6121 ctggcaggct gggcaataga aggtcgagcg gttgctctgc acgattcggc gcacgatgtg
     6181 gccgcgcgcc gcgcgcgggc aggcctcgcc ggcccggtcg tagacgctga aattgtgctg
     6241 gaagtagccg agctcgccgt tcgcctgcac gtagtcgcgc aacgacgagc caccggccgc
     6301 gatcgcctcc tccagcaccg ccccgatcgc cgccgcgagg cgcgcggcgc gcacgccggc
     6361 gaccccggcg ccgctccggc gcggcgagat gccggcccgg tacaggctct cgcaggcata
     6421 gatgttgccg atgccggcga ccaggcgctg atcgagcagc agcgccttga tcggcgcccg
     6481 gcgcccgcac agcgccgcgc tcaacacctc ggcgtcgaag tcggggccga gcgggtcggg
     6541 cccgagatgg cggagcagcg gatggcgggc gagcgccctg ctcggcgcga gatcgaacag
     6601 gccgaaccgg cgggcgtcgt tgaacaccac ctcgctgccg tcgtcgatcg cgaacaccac
     6661 gtgctcgtgc ggctgggccg cgcacgtccc gcccgggcgc aggtacagcc gccccgacat
     6721 gccgagatgg gcgatcaggg tctcgcccga gtcgagcccg atcagcaggt atttggcccg
     6781 ccgcgccacc tcgatgaccg cgcgcccggc aagtcggcgg gcgaagtcgc gggcgagcgg
     6841 ccggcgcagg tcgccgcggc ggacggtcac ggcggtgatc cgccggccga tcagccgcgg
     6901 cgccagcccg cggcggatgg tctcgatttc gggcagctcg ggcatggcgc ggaatgaggc
     6961 tgttggcggg cggcggcgac tgtagcgtgg cctcgggcgc tgcgctatgg tcgccgccat
     7021 gtccgcgcga ctcgggccga aggcgccggc ggcagggcca ccggaaaccg tgccgttcgg
     7081 ccgcctggac gtggcgccgg ccgacaagcc ggcgctggtc gcccgcctgt tctcgggcgt
     7141 cgcggcacgc tacgacctga tgaacgacct gatgagcgcc ggcgtccatc ggctgtggcg
     7201 gcgcgcgctg gtggcggcgc tcgagctcaa gccgggcgcg catgtcctcg acctcgccgg
     7261 cggcaccggc gacatcgcgc ggcgaatcct cgaggccgac gacggcgcgc gcggcgtcgc
     7321 ggtcacggtc tgcgacctga ccccggcgat gctcgccgag ggccggcggc gggcgtggga
     7381 ccgcggccgg gcctcgggtc tcgcctgggt ctgcgccgag gcggcgcggc tgccgttcgc
     7441 ggcgcgcagc ttcgatgcct gcacgctcgc cttcgggctg cgcaacatga cccggccgga
     7501 gccggcgctg gccgaggtcc ggcgcgtgct gaggccgggc gcgcgtttcc tgtgcctaga
     7561 gttcagcccc gacgtggcac cggcgctggc gcccgtctac gacgccgtca ccgggcgcgt
     7621 gatcccggcg ctcggcgaga tcgtcgccgg cgaccgcgag gcctaccagt acctggtcga
     7681 cagcatccgc cgcttcccga cctcggcacg cctcgccgag atgatcgccg aggccgggtt
     7741 cgccggcgtg cgcgcgcgcg ccttcgccgg cggcatcgcg gcgctccatt cggcgtggcg
     7801 cgcctgaggc cgggccggtg atcggggggt gatccgcacc gcgcgcaacc tgtcgcggct
     7861 ggccgcgatc gcggtcacgc tcgcccgcca cgacgcgctc gcgccgctcg aggcgctggg
     7921 ggtggcgccg tggctggtat ggctggtccg ccgcctcgcc cgccgcgcgg cgcgcgaggc
     7981 gcgcatcggc cagcgcctgg ccggcgccgc ggtcgcgctc ggcccgggct tcatcaagct
     8041 cggccaggcg ctgtcgaccc gcgccgacct gctcggcacc gaggtcgccg aggacctggc
     8101 gatgctgcgc gaccggctgc cgccgttccc ggcggcggag gcgcgcgcgg tggtcgcgcg
     8161 cgagctgggg gtggcggtgg acgaggcgtt cgccaccttc gacgacgtcc cggtcgccgc
     8221 ggcgtccatc gcccaggtcc atttcgccac caccgcggac gggcgcgagg tcgcggtcaa
     8281 ggtgcagcgc ccgggcatcg agcaggcgtt ccggcgcgac ctcgacctga tggtctggct
     8341 cgcgcgcctg atcgagcgca cgagcgcgcg ctggcgccgc ctgcgcccgg tcgagtccgt
     8401 gcgcaccttc gccgagatgg tggcgatcga gatggacctg agcctcgagg gcgcggcggc
     8461 gtccgagctc ggcgccaact tcgccgacga tccgagcttc aacgtgcccg cggtcgactg
     8521 gcagcgcacc gcgcgccggg tgctgaccac cgaccgggtg ccgggcatcg ccatcgaccg
     8581 gcgcgaggcg ctgatcgccg ccggccacga cccggcggag ctcttggcca aggcggcgcg
     8641 ggcgaccttc ctgcaggtgt tccgcgacgg cttcttccac ggcgatcccc acccgggcaa
     8701 cctgttcgtc gacgccgccg gcaatttatg ggtggtcgat ttcggcatca tgggccggct
     8761 cgacattgcc acccgcaggt ttttgggcga gatgctgacc ggcttcctca acggcgacta
     8821 tgaggcggtc gccgacgtcc acttccgcgc cggattcgtc cccgccgggc gctcgcgcgg
     8881 cgccttcgtc caggcactgc gcgcgatcgg cgagccgatc ctgggcctgc ccgcgaaccg
     8941 catctcggtc ggccgcctgc tcggccggct gttccaggtc accgagacct tcggcatgca
     9001 gacccagccg cacctgctgc tgctgcagaa ggtgatggtg gtcgccgagg gggtcgggcg
     9061 cgtgctctat ccggacgcca acatgtggca gctcgccagg cccttgatag aggactggat
     9121 ggccgcgcag tcggggctgg aggcacgcct gggcgccgcc gccgaggccg tcggcgagat
     9181 cgcgcgccgg ctgccccgga tcgccctcca ggccgagcgc gcgctcgacc gcctggcggc
     9241 gggcccggag cccgaggcgg cgcgcgcgcg ccgggcggat gcgcgcatcg cgctcgcctt
     9301 cgccctcggc ctcgccgccg gggcgctggt cgcgctcgcc gcgttggatt gaccgatcgg
     9361 ttgccaaagg ggcgggccgt gcctagcttt acagcaccgg cacggcggcc cggcggcgcc
     9421 gcctgaaggc tccccgcgat gcagcccttc gaaggcaagc gcattctgtt gatcgtcgcg
     9481 ggcggcattg cggcctacaa gagccttgag ctggtccgcc gcctgcgcga gcggggcgcg
     9541 gcggtgcgca cggtgctgac cgcgggcggc gccgagttcg tgaccccctt gagcctcgcc
     9601 gccctcagcg aggacaaggt ctatcgggac ctgttctcgc tcaccgacga gagcgagatg
     9661 gggcatatcc gcctctcgcg cgaggccgac ctggtggtgg tcgcgccggc gagcgccgac
     9721 ctgatcgcca agatggccgc cgggcttgcc gacgacctcg cctcgacggt gctgctggcg
     9781 accgacaccc cggtgctgat cgcgccggcg atgaaccaca tgatgtggca gcacccggcc
     9841 acccggcgca acatcgccac ccttgcccgc gacggcgtgc gccggatcgg gcccgaggcc
     9901 ggcgacctcg cctgcggcga ggtcggcgcc gggcggatgg ccgagccggc ggcgatcctg
     9961 gccgagatcg agcgcctctt ggccgcggat gcggaccggc cgctgcatgc ggtgcgggcg
    10021 ctggtcacca gcggcccgac ccatgagccg atcgatccgg tgcgctacct cgccaatcgt
    10081 tcgtcgggca agcagggcca tgcgatcgcg cgcgccatcg gcgcgctcgg cgccgaggtg
    10141 accctggtca ccggcccgac cgcgctcgcc gacccggcgt gcgcgcgcgt ggtccgggtc
    10201 gagaccgccc aagagatgct ggccgcatgc gaggcggcgc tgccggtcga cgtcgcggtg
    10261 tgcgccgcgg cggtcggcga ctggcggccc gcgcgcccgg ccaaccggaa gatcaagcgc
    10321 gacggcggcg gcccgccggc gctcgatctg gccgagaacc ccgacatcct cgccaccctc
    10381 gccgggcgca agcgcggccg cccgaccctg gtcatcggct tcgccgccga gaccgagcgg
    10441 gtggtcgaga acgccaccgc caagcgcgcc gccaagggct gcgactggat cctggccaac
    10501 gacgtgtccg caggcaccgg cgtgttcggc ggcgatgaga ccaccgtcca cgtcatcgac
    10561 tcggccggcg ccgagacctg ggccaaggcg tcgaaggacg aggtcgccga gcggctcgcg
    10621 cgccggatcg cggcggcgat cggggagcgg gggatcaccg catgaccgcc ggcgcggcgg
    10681 tcgcggtcag cgtgcgccgc ctcgaccacg ccctcgacgg gccgttgccg ggctatgaga
    10741 ccgagcacag cgcgggcgtc gatctggccg cggcggtggc cgaggagctg gtcatcgccc
    10801 ccggcggacg cgcgctggtg ccgaccgggc tcgcgctcgc gctgccgccg gggttcgagg
    10861 cgcaagtcag gccgcgctcg gggctcgcgc tccgccacgg aattaccgtg ctcaacagcc
    10921 cgggcacgat cgatgccgac taccgcggcg agatcggcgt ggtgctggtc aaccacggcg
    10981 gcgagccgtt caccgtccgc cgcggcatgc gcgtcgccca gctggtggtg gcgccggtca
    11041 gccggctcgc ctggcaggag gtggcgctg
//
LOCUS       DASWHU010000051         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2236966, whole genome shotgun sequence.
ACCESSION   DASWHU010000051 DASWHU010000000
VERSION     DASWHU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236966"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGB88_01950"
     CDS             complement(<1..287)
                     /locus_tag="VGB88_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646230.1"
                     /translation="MAGPGGTSSRGRIAALLFLLALALLALVVLRAYESHRGETVVAE
                     VPLLRADPSPVRERPADPGGMQVPYQDSLILENLGEETPAPTVERLLPPPE"
     gene            complement(288..2030)
                     /gene="argS"
                     /locus_tag="VGB88_01955"
     CDS             complement(288..2030)
                     /gene="argS"
                     /locus_tag="VGB88_01955"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9646231.1"
                     /translation="MDIFKRFGGHIQEALAELAAEGAVPGGLDAGGVAVEPPRDPAHG
                     DLATNAALVLAKPAGMRPRELAGLIAPRLARIEDVVAAEVAGPGFINLRLATRAWTRV
                     LGDILEEGGDYGRSQVGASRKVNVEFVSANPTGPMHVGHSRGAVFGDALAALLEFTGH
                     QVTREYYVNDAGAQIDALARSTWLRYREALGEAIGEIPSGLYPGDYLRPVGEALAARD
                     GARWREAGEAERLEACREAALKAMMAEIADDLAALGVRMDRFTSERALIAAGRVDQVL
                     ATLQARGLIYTGVLDAPKGKLVEDWEPRPQTLFRSSQFGDDVDRPLKKSDGSWTYFAS
                     DIAYHLDKVERGFADMIDVWGADHAGYVKRMKAAVAAVSEGRAALDVKICQLVNLLDG
                     GRPVKMSKRAGTFVTLREVVDTVGKDVVRFIMLTRRNDAPLDFDFARVTEKSRDNPVF
                     YVQYAHARIRSVERKAAALPGLAAAAGEDDRARLEHPDELALIRMLAGWPRVVEAAAL
                     AHEPHRLAFYLYDLASAFHALWNKGNDDPGLRFMVEDDLQLTRARLALIGGVKQVIAA
                     GLGVIGVAPAEEMR"
     gene            complement(2030..3193)
                     /locus_tag="VGB88_01960"
     CDS             complement(2030..3193)
                     /locus_tag="VGB88_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9646232.1"
                     /translation="MSRVPGAPFATSPDSSRGRLHPEAESPSRTPFQRDRDRIVHSAA
                     FRRLEHKTQVFVHHEGDHYRSRLTHSLEVAQIARSISRELGLNEDLAEGLALAHDLGH
                     TPFGHAGEDALSEAMTPFGGFDHNSQTLRIVTRLERRYAGFDGLNLTWETLEGVVKHN
                     GPMDPGHLPETVAAYLADHDLEVATWPGPEAQVAALSDDIAYNNHDIDDALRAGLFEI
                     ADLAAVPLAGPVFAEVEARYPGLERPRLIHESVRRLINAMVCDLVAESRRRVAEYAPK
                     SVEAVRVLGAPLVGFSASMRADSEALKGFLFANMYRHFRVNRMASKARRVVSDLFALF
                     MAEPDCLPTQWQRRAGAPGSRECARLVADYVAGMTDRYALKEHARLFDVSAQI"
     gene            3344..3703
                     /gene="erpA"
                     /locus_tag="VGB88_01965"
     CDS             3344..3703
                     /gene="erpA"
                     /locus_tag="VGB88_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9646233.1"
                     /translation="MTEIKPRSAADFGMTMTARAAERIKALRTAEGESTRLRLEVVGG
                     GCSGFQYTFSLADHDNDDDLRIERDGATIVVDETSVSLLQGSEVDFHEGLDGSMFTVS
                     NPNATASCGCGTSFAVG"
     gene            3876..>4342
                     /locus_tag="VGB88_01970"
     CDS             3876..>4342
                     /locus_tag="VGB88_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9646234.1"
                     /translation="MIVATWNVNSVKARLANLLDWLDEARPDVALLQETKTVDEAFPR
                     LEVEERGYNCALAGQKTYNGVAILSKYPLEDVRGALPGDRKDDQARYVEAWVDAAGAG
                     VRVASIYLPNGNPVDSDKYPYKLAWMDRLRRHAAALLAPEEAVVLGGDYNVIPA"
BASE COUNT          631 a         1573 c         1521 g          617 t
ORIGIN      
        1 tgctccggcg gcggcagcag ccgctcgacg gtcggggcgg gcgtctcctc gccgaggttc
       61 tccaggatca gcgagtcctg gtagggcacc tgcatgccgc cgggatcggc cgggcgctcg
      121 cgcaccggcg aggggtcggc gcgcagcagc ggcacctcgg cgaccacggt ctcgccgcgg
      181 tgcgactcgt aggcgcgcag cacgaccagc gcgagcagcg ccagcgccag caggaacaac
      241 agcgccgcga tccgccctcg gctcgacgtc ccgcccggcc ccgccactca gcgcatctcc
      301 tccgccggcg ccaccccgat cacgcccagc cccgcggcga tcacttgctt gacgccgccg
      361 atcagggcga ggcgcgcgcg ggtgagctgg aggtcgtcct cgaccatgaa ccgcaggccg
      421 gggtcgtcgt tgcccttgtt ccacagcgcg tggaacgccg aggccaagtc ataaagatag
      481 aacgccagcc ggtgcggctc gtgcgccagc gccgccgctt cgaccacgcg cggccagccg
      541 gcgagcatcc ggatcagcgc aagctcgtcc gggtgctcga ggcgcgcccg gtcgtcttcg
      601 cccgcggcgg cggcgagccc gggcagcgcg gccgccttgc gctcgaccga gcggatgcgc
      661 gcatgggcgt attggacgta gaacaccggg ttgtcgcgcg acttctcggt cacccgcgcg
      721 aagtcgaagt cgagcggcgc gtcgttgcgc cgggtcagca tgatgaaccg aaccacatcc
      781 ttgccgacgg tgtcgaccac ctcgcgcaag gtgacgaagg tgccggcgcg cttcgacatc
      841 ttcaccggcc ggccgccgtc gagcaggttg accagctggc agatcttgac gtcgagcgcc
      901 gcccgcccct cgctcaccgc cgccaccgcc gccttcatgc gcttgacgta gccggcgtgg
      961 tcggcgcccc agacgtcgat catgtcggcg aagccgcgct cgaccttgtc cagatgatag
     1021 gcgatgtcgg aagcgaagta ggtccagctg ccgtcggact ttttgagcgg gcggtcgacg
     1081 tcgtcgccga actggctgga gcggaacagg gtttgcggcc tgggctccca gtcctctacc
     1141 agcttgccct tgggcgcatc gagcacgccg gtgtagatca gcccgcgcgc ctggagcgtg
     1201 gccagcacct ggtcgacccg cccggccgcg atcagcgcgc gctcggacgt gaaccggtcc
     1261 atgcgcacgc cgagcgccgc caggtcgtcg gcgatctctg ccatcatcgc cttgagcgcg
     1321 gcctcgcggc acgcctccag ccgctccgcc tcgcccgcct cgcgccagcg cgcgccgtcg
     1381 cgcgccgcca gcgcctcgcc caccgggcgc aggtagtcgc ccggatagag cccggacggg
     1441 atctcgccga tcgcctcgcc gagcgcctcg cggtagcgca gccaggtcga gcgcgccagc
     1501 gcatcgatct gggcgccggc gtcgttgacg tagtactcgc gcgtcacctg gtggccggtg
     1561 aactccaata gcgcggcaag cgcatccccg aacaccgcgc cgcggctgtg cccgacatgc
     1621 atcggcccgg tcgggttggc cgagacgaac tcgacattga ccttgcgcga agccccgacc
     1681 tggctccgcc cgtagtcccc gccctcttcc agaatgtcgc cgaggacgcg cgtccaggcg
     1741 cgcgtcgcca ggcgcaggtt gatgaatccc ggccccgcga cctcggccgc gaccacgtcc
     1801 tcgatgcgcg cgagcctcgg cgcgatcagg ccggcaagct cgcgcgggcg catcccggcc
     1861 ggcttcgcca gcaccagggc ggcgttggtg gcaaggtcgc catgggccgg gtcgcgcggc
     1921 ggctcgaccg ccaccccgcc ggcatcgagc ccgccgggga ccgcgccctc ggcggcgagc
     1981 tcggcaagag cttcctggat gtggccgcca aaccgcttga agatgtccat cagatctgtg
     2041 ccgagacatc gaatagccgg gcatgctcct tgagcgcgta gcggtcggtc atgccggcga
     2101 cgtaatcggc gaccagccgc gcgcactcgc gcgacccggg tgctccggcg cggcgctgcc
     2161 actgggtcgg caggcagtcg ggctcggcca tgaacagcgc gaacaggtcg ctcaccaccc
     2221 ggcgcgcctt gctggccatc cggttgacgc ggaagtggcg gtacatgttg gcgaacagga
     2281 accccttcag cgcctcgctg tcggcgcgca tcgaggccga gaacccgact agcggcgcgc
     2341 ccaatacgcg caccgcctcg accgatttgg gcgcgtattc cgccacccgg cgccggctct
     2401 cggcgaccag gtcgcagacc atggcgttga tcaagcggcg gaccgattcg tggatcaggc
     2461 gcgggcgctc gaggccggga tagcgcgcct cgacctcggc gaacaccggc ccggcgagcg
     2521 gcaccgccgc gaggtcggcg atctcgaaca ggccggcacg cagcgcgtcg tcgatgtcgt
     2581 ggttgttgta ggcgatgtcg tcgctgagcg ccgccacctg ggcctccggc ccgggccagg
     2641 tcgcgacctc gaggtcgtgg tcggcgaggt aggccgcgac cgtctcgggc aggtgcccgg
     2701 gatccatggg accgttgtgc ttgaccaccc cttcgagtgt ctcccaggtc aggttgaggc
     2761 cgtcgaaccc ggcgtagcgg cgctcgagcc gggtgacgat gcgcagggtt tgagagttgt
     2821 ggtcgaagcc gccgaacggc gtcatcgcct cgctcaaggc gtcctcgccc gcgtgcccga
     2881 agggtgtatg ccccagatca tgggccagcg ccagaccctc ggccaggtcc tcgttcaggc
     2941 ccagctcgcg gctgatcgag cgcgcgatct gcgccacctc caggctgtgg gtgagccggc
     3001 tgcggtagtg gtcgccctcg tgatggacga acacctgggt cttgtgctcg agccggcgga
     3061 acgccgccga atgcacgatc cggtcgcgat cgcgctggaa cggcgtccgc gacgggcttt
     3121 cggcttccgg atggaggcgg ccccggctcg agtccggact ggtcgcgaac ggcgcgcctg
     3181 ggacgcggct catggcgcga acctagccat cgggccgtcc ggattcaacg ccgccgcggg
     3241 ccaaggcggg cgccgattga caaggcccgg cgcgggacca aaataggctt caaggacggc
     3301 cgggcgcccg cgcccgtggc cggccgcgaa aatcgaggtt cgtatgacgg agatcaagcc
     3361 caggtcggcg gccgatttcg gcatgaccat gaccgcgcgc gcggccgagc gaatcaaggc
     3421 cctgcgcaca gctgagggcg aatccacccg gctgcgcctg gaggtggtcg gcggcggctg
     3481 ctcgggcttc cagtacacct tctcgctcgc cgaccacgac aacgacgacg acctcaggat
     3541 cgagcgcgac ggcgccacga tcgtggtcga cgagacctcc gtcagcctcc tgcagggctc
     3601 ggaggtcgat ttccacgagg gtctcgacgg gtcgatgttc acggtgagca accccaacgc
     3661 cacggcgtcc tgcggctgcg gcacctcctt cgcggtcggc tgagcgccca ggtggccagc
     3721 gcaggcgcag cctccgctct cgtgcagtcg cacccccacc caagccctcc ctcgcccgcg
     3781 ggggagggtt ttgctttttt gaacctccgc ccgcttgcgg ggggaggcag gaggggggcc
     3841 gatggtcgtg cccgggccgg gcgtttgcgc gcgcgatgat cgtcgccacc tggaacgtca
     3901 actcggtcaa ggcgcggctc gccaacctgc tcgactggct ggacgaggcc aggcccgatg
     3961 tcgccctgct ccaggagacc aagaccgtcg acgaggcgtt cccgcggctc gaggtcgagg
     4021 agcgcggcta caactgcgcg ctggccggcc agaagaccta taacggcgtc gccatcctgt
     4081 ccaagtaccc gctggaggac gtgcgcggcg cgctgcccgg cgatcgcaag gacgatcagg
     4141 cccgctacgt cgaggcgtgg gtcgacgccg ccggcgccgg cgtgcgcgtg gcctcgatct
     4201 acctgcccaa cggcaacccg gtcgacagcg acaagtaccc ctacaagctc gcctggatgg
     4261 accggctgcg ccgacacgcc gccgcgttgc tcgcgcccga ggaggcggtg gtgctgggcg
     4321 gcgactacaa tgtcatcccg gc
//
LOCUS       DASWHU010000052         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_389678, whole genome shotgun sequence.
ACCESSION   DASWHU010000052 DASWHU010000000
VERSION     DASWHU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389678"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VGB88_01975"
     CDS             complement(<1..970)
                     /locus_tag="VGB88_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9646235.1"
                     /translation="MAIRVRAEPLRDLAADIFTAAGCPAEEGARIARYLVEANLTGHD
                     SHGVIRVPRYVHWLKQGYVVPGQTVEVVVDAGALAVVDGRYGFGQTVGEQAVDLGLDK
                     ARAHGVSVIGLRNSGHLGRVGDWPERAAACGFASVHFVNTTGLGMLVAPFGGRERRMS
                     TNPFAVGVPAGDGPPVILDFATSIVAEGKILVAVNGGKPLPRDAFVDADGSFTTDPEV
                     IYGKLDGTQPLDARTGAGAIRAMGEHKGSGLSIMCELLGGALTGGGCARPGETRIVNG
                     MLSIYIDPARLDAAGSFAGEVARYLDFARSAKPIEADGTVMMPGDPE"
     gene            complement(972..2042)
                     /locus_tag="VGB88_01980"
     CDS             complement(972..2042)
                     /locus_tag="VGB88_01980"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX9646236.1"
                     /translation="MGIGSDGLKALTAAIFSNTGSGGAEAEAIARHLVEANLVGHDSH
                     GVIRVPAYVQWVGEGKLVPNQSISVAFENDSVAIVDGNSGFGQVIGEQAVDLGIDKCR
                     TRGLAMVGLRNAGHIGRIGDWAIRAADAGLVSIHFVNTSGGGILVAPFGGTDRRLSAN
                     PIAAGVPLPGRRPIVVDMSTCVIAEGKIKVALNKGVQVPENAIVDGYGKPTTDPAAFY
                     REPPGAILTIAAHKGYTLSVLCEVLAGAITGGGCSNPANPTARKIVNGMLSIYIDPAT
                     VAGAGFAGEIERLIDWVKASPKATPGGTILMPGEIEDATKAERLKGGIPLDDMTWRQM
                     VETARTVGLADSDIERLSGRGV"
BASE COUNT          302 a          803 c          783 g          343 t
ORIGIN      
        1 tctcggggtc gccgggcatc atcaccgtgc cgtccgcctc gatcggcttg gcgctgcgcg
       61 cgaagtcgag gtaacgcgcg acctcgccgg cgaagctgcc ggcggcatcc aggcgcgcgg
      121 ggtcgatgta gatcgacagc atgccgttga cgatccgcgt ctcgcccggc cgcgcgcagc
      181 cgccgccggt cagcgcgccg cccaacagct cgcacatgat cgacagcccc gagcccttgt
      241 gctcgcccat cgcgcggatc gcgcccgcgc cggtgcgcgc atcgagcggc tgggtgccgt
      301 cgagcttgcc gtagatcacc tcggggtcgg tggtgaagct gccgtcggcg tcgacgaagg
      361 catccctcgg cagcggcttg ccgccgttga ccgcgaccag gatcttgccc tcggcgacga
      421 tcgaggtggc gaagtcgagg atcaccggtg gcccgtcccc ggccggcacc ccgaccgcga
      481 acgggttggt cgacatgcgc cgctcgcggc cgccgaacgg cgccaccagc atgccgaggc
      541 cggtggtgtt gacgaagtgg accgaggcga agccgcacgc cgcggcgcgc tccggccagt
      601 cgccgacccg cccgagatgg cccgagttgc gtaagccgat caccgacact ccgtgcgccc
      661 gcgccttgtc gaggccgagg tcgaccgcct gctcgccgac cgtctggccg aagccgtagc
      721 ggccgtcgac cactgccagc gcgccggcgt cgaccaccac ctcgacggtt tggccgggca
      781 ccacgtaacc ctgcttgagc cagtgcacgt agcgcggcac gcggatcacg ccgtggctgt
      841 cgtggccggt gaggttggcc tcgaccaggt aacgcgcgat gcgtgcgcct tcctcggccg
      901 ggcagccggc ggcggtgaag atgtccgcgg cgaggtcgcg cagcggctcg gcccgcaccc
      961 ggatggccat gtcagacgcc gcgccccgac agccgctcga tgtcggagtc ggcgaggccc
     1021 acggtgcgcg cggtctcgac catctgccgc caggtcatgt cgtcgagcgg gatgccgccc
     1081 ttgagccgct cggccttggt cgcgtcctca atctcgcccg gcatcaggat ggtcccgccc
     1141 ggggtcgcct tgggcgatgc cttgacccag tcgatcaggc gctcgatctc gccggcgaag
     1201 cccgcgccgg cgaccgtggc cgggtcgatg tagatcgaca gcatgccgtt gacgatcttc
     1261 ctggcggtcg ggttggcggg gtttgagcag ccgccgccgg tgatcgcgcc ggccagcacc
     1321 tcgcacagca ccgacagcgt atagcccttg tgggcggcga tggtcaggat cgccccgggc
     1381 ggctcgcggt agaacgccgc cgggtccgtg gtcggcttgc cgtagccgtc gacgatagcg
     1441 ttttcaggaa cttgcacgcc cttgttaagc gctaccttga tcttgccttc ggcgatcacg
     1501 caggtcgaca tgtcgaccac gatcgggcgc cggccgggga gtggcacacc ggcggcgatc
     1561 gggttcgccg acagccggcg gtcggtgccg ccgaacgggg ccaccaggat gccgccgccc
     1621 gaggtattaa cgaagtggat cgacaccagg ccggcatcgg cggcgcggat cgcccagtcg
     1681 ccgatgcgcc cgatatgccc ggcattgcgc agtccgacca tcgccagccc gcgggtccgg
     1741 cacttgtcga tgccgaggtc gaccgcctgc tcgccgatca cctggccgaa gcccgagttg
     1801 ccgtcgacga tcgccaccga gtcgttctcg aacgccaccg agatcgactg gttgggcacc
     1861 agcttgccct cgccgaccca ctggacgtag gccgggaccc ggatcacgcc gtggctgtcg
     1921 tggccgacca ggttggcctc gaccaggtgc ctggcgatgg cctcggcctc ggcgccgccg
     1981 ctgccggtgt tggaaaagat cgccgccgtc agggccttca gcccgtcgct cccgatgccc
     2041 atgcctgtcg accccgcatg cttagttgtt ggttggtcgt tgcgcgcgct ctcttagccg
     2101 tctcaccctg cacgggcaag ccgacaatgc cgggatcacc cggcccgggc cttgcgcgcg
     2161 cggcgcagca gccggctggc gatggcgtgg cggtggacct cggacgggcc gtcgtagatt
     2221 cggaatgggc g
//
LOCUS       DASWHU010000053         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2604452, whole genome shotgun sequence.
ACCESSION   DASWHU010000053 DASWHU010000000
VERSION     DASWHU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604452"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VGB88_01985"
     CDS             complement(<1..1196)
                     /locus_tag="VGB88_01985"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9646237.1"
                     /translation="MTELRELAMHSNAWPFLEARTLLDRIAAMARPPAQVLFETGYGP
                     SGLPHIGTFAEVARTTMVRRAFELISDVPTRLFAFSDDMDGLRKVPDNIPNRELVAAH
                     LGKPLTAIPDPFGSHDSFGAHNNARLRAFLDDFGFDYDFKSATECYRTGTFDATLLEV
                     LKHYDAVTEVILPTLGPERRATYSPFLPICARTGRVLQVPVLARDADAGTITYQDEDG
                     GRVEVPVTGGRCKLQWKADWAMRWRALGVDYEMSGKDLISSVELSGRICRILGGAPPL
                     GFTYELFLDEQGQKISKSKGNGLTIDEWLAYGSRASLSLYMYHSPRKAKRLTFDVIPR
                     HVDEYLSQLARYREQEPAERLQNPVWHVHAGAPPADDVPLTYNVLLNLASVCNTEDKQ
                     VLWGFIA"
     gene            1288..4062
                     /locus_tag="VGB88_01990"
     CDS             1288..4062
                     /locus_tag="VGB88_01990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9646238.1"
                     /translation="MAARRRPASIAALIAGAPTLVAGTQGAAWLGPDGELAELTLKGA
                     AARARDGPAPIVCHLPATARRLGIGPFPALDALELLAFTRPAQFCVPTARGLAAAAGL
                     DAPEGLADQAASLREAALTLLAELARADYPDAAEAAAVARRLAEAGWSWGPAVVGALG
                     IEARASRLDVWAKLPEWSEAAPEPAPGHFPVDPDEARTRLAALLGAGAEPRPQQADYA
                     SAATFAFAPRARRGRPRVVLAEAGTGVGKTLGYIAPASVWADKNGGAVWLSTYTKNLQ
                     RQIDRELDRLYPDRALKARKVVVRKGRENYLCLLNFEEAVARLGEASLPGFGGAAVAL
                     GLLARWARHSRDGDMVGGDFPGWLAEVIGPARGRGLTDRRGECIYAACPHWRRCFIER
                     ASRRAHAAELVIANHALVMAQAAARDNEPYLPMRYVFDEGHHLFDAADGAFSVHLSGQ
                     ETRELRRWLRGAEAGRRTRARGLRRRIGDLVEGDDTAAGALQEVLAAARALPGDGWLR
                     RIAEGRADGPAEGFLAHVHQQVAARAQDARGFSLETDARPPVDGLIEAADALESALSL
                     LARPMERLQQALARRLDAEADTLETATRTRLEAAARTLRRRREMAVAPWRAMLKSLAG
                     ALPEAFVDWLSIERRDGQVLDVGMHRHWVDPTEPFAAAVLAPAHGALITSATLRDGTG
                     DVEADWAAAEARTGAAHLAEPALRAAVPSPFDYAAQTRALVVTDVDRDDPDVTAAAYR
                     ELFLAAGGGALGLFTAIWRLRRIHARIAGPLEEAGLVLLAQHVDAMDTGTLVDIFRAE
                     RNACLLGTDALRDGVDVPGEALRLIVFDRVPWPRPDILHKARRARFGGRAYDDMITRL
                     RLRQAFGRLVRRADDYGVFVMLDAATPSRLYGAFPDGVEVTRAGLAEAVQTTRTFLAE
                     HARTRPPSVA"
BASE COUNT          508 a         1548 c         1526 g          568 t
ORIGIN      
        1 gcgatgaacc cccacaggac ctgcttgtcc tcggtgttgc acacgctggc gaggttgagc
       61 agcacgttgt aggtgagcgg cacgtcgtct gcgggcgggg cgccggcgtg gacgtgccag
      121 accgggttct gcaggcgctc cgccggctcc tgctcgcggt acctggcgag ctggcttaag
      181 tattcgtcga catggcgcgg gatgacgtcg aaggtgagcc gcttcgcctt gcgcgggctg
      241 tgatacatgt acagcgacag gcttgcgcgc gaaccgtaag ccagccactc gtcgatggtc
      301 agcccgttgc ctttggactt cgagatcttc tggccctgct cgtcgaggaa cagctcgtag
      361 gtgaagccga ggggcggcgc gccgccgagg atgcggcaga tgcgcccgga cagctcgact
      421 gacgagatca ggtccttgcc cgacatctcg tagtcgacgc cgagcgcgcg ccagcgcatc
      481 gcccagtcgg ccttccattg cagcttgcag cgcccgccgg tgaccggcac ctcgacgcgg
      541 ccgccgtcct cgtcctggta ggtgatggtg ccggcgtccg catcgcgcgc gaggaccggc
      601 acctggagca cccgcccggt gcgcgcgcag atcggcagga acgggctgta ggtcgcccgc
      661 cgctcgggcc cgagggtggg caggatcacc tcggtcaccg cgtcgtagtg cttgagcacc
      721 tccagcaagg tggcgtcgaa ggtgcccgtg cgatagcact cggtcgcgct cttgaaatcg
      781 tagtcgaagc cgaagtcgtc gaggaacgcg cgcaggcggg cgttgttgtg ggcgccgaag
      841 ctgtcgtggc tgccgaacgg gtcggggatc gcggtcagcg gcttgccgag atgggccgcc
      901 accagctcgc ggttggggat gttgtcgggc accttgcgca gcccgtccat gtcgtcggag
      961 aacgcgaaca gccgggtcgg cacgtcgctg atcagctcga acgcgcgccg gaccatggtg
     1021 gtgcgcgcga cctccgcgaa ggtgccgatg tgcggcagcc ccgacggccc gtagccggtc
     1081 tcgaacagca cctgggccgg cgggcgcgcc atcgccgcga tgcggtcgag cagcgtgcgc
     1141 gcctcgagga acggccaggc gttgctgtgc atggcgagct cgcgcaattc ggtcatggtg
     1201 agcgccccgg gatgcgcgcg accctaggtc gcacggccat ccccgtcaat gcgcgccgcc
     1261 gcccgccgcg gctatactcc gccggccatg gccgcccgcc gcagacccgc ctccatcgcc
     1321 gccctgattg cgggcgcgcc gaccctggtc gcgggcacgc agggcgccgc ctggctcggg
     1381 cccgacggcg agctcgccga gctcacgctc aagggcgcgg cggcgcgcgc ccgcgacggc
     1441 ccggcgccga tcgtctgcca tctgccggcg accgcgcgca ggctcggcat cgggccgttc
     1501 ccggcgctcg atgcgctcga gctgctggcg ttcacccgcc cggcgcagtt ctgcgtgccg
     1561 accgcgcgcg ggctcgccgc cgcggccggg ctggacgcgc ccgagggtct cgccgaccag
     1621 gcggcgagcc tgcgcgaggc ggcactcacc ctgctcgccg agctcgcccg cgccgactac
     1681 ccggacgccg ccgaggcggc cgcggtcgcc cgccgcctcg ccgaggcggg ctggtcgtgg
     1741 gggccggcgg tggtcggcgc gctcggcatc gaggcgcgcg cctcgcggct cgacgtgtgg
     1801 gcgaagctgc ccgagtggag cgaagccgcg cccgagcccg cgcccggcca tttcccggtc
     1861 gatcccgacg aggcgcgcac gcgcttggcc gcgctcctcg gcgccggcgc cgagccgcgc
     1921 ccgcagcagg ccgactacgc ctcggccgcg accttcgcct tcgccccgcg cgcgcgccgc
     1981 ggccgcccgc gcgtggtcct cgccgaggcc ggcaccgggg tcggcaagac cttgggctac
     2041 atcgccccgg cgagcgtgtg ggcggacaag aacggcggcg cggtgtggct gtcgacctac
     2101 accaagaacc tgcagcgcca gatcgaccgc gagctcgacc ggctgtaccc cgaccgggcg
     2161 ctgaaggcgc gcaaggtggt ggtgcgcaag ggacgagaga actacctgtg cctgctcaat
     2221 ttcgaggagg cggtggcgcg cctcggcgag gcgtcgctgc cgggattcgg cggcgcggcg
     2281 gtggcgctcg gcctgctcgc gcgctgggcg cgccacagcc gcgacggcga catggtcggc
     2341 ggcgacttcc cgggctggct ggccgaggtg atcgggccgg cgcgcgggcg cggcctcacc
     2401 gaccggcgcg gcgagtgcat ctacgccgcc tgcccgcact ggcggcgctg cttcatcgag
     2461 cgcgcgagcc ggcgcgcgca cgccgccgag ctggtgatcg ccaaccacgc gctggtgatg
     2521 gcccaagccg cggcgcgcga caacgagccg tacctgccga tgcgctacgt gttcgacgag
     2581 ggccaccacc tgttcgatgc cgccgacggc gcgttctcgg tgcacctgtc ggggcaggag
     2641 acgcgcgagc tcaggcgctg gctgcgcggc gccgaggcgg ggcggcgcac ccgcgcgcgc
     2701 ggcttgcgcc ggcgcatcgg cgacctggtc gagggcgacg acacggcggc gggcgctctc
     2761 caagaggtgc tcgccgcggc gcgcgcgctg cccggcgacg gctggctcag gcgcatcgcc
     2821 gagggccgcg ccgacggccc ggctgagggc tttctcgccc atgtccacca gcaggtcgcg
     2881 gcgcgcgccc aggacgcgcg cggcttcagc ctggagaccg atgcccgccc gccggtcgac
     2941 ggcctgatcg aggccgccga cgcgctcgag tccgcgctca gcctcctggc gcggccgatg
     3001 gaacgcctcc agcaggcgct ggcccgccgg ctcgacgccg aggccgacac gctggagaca
     3061 gcgacccgca cccgcctcga ggccgccgcg cgcacgctgc ggcgccggcg cgagatggcg
     3121 gtcgcaccct ggcgggcgat gctcaagtcg ctcgccggcg cgctgcccga ggcgttcgtc
     3181 gactggctgt cgatcgagcg ccgcgacggc caggtcctcg acgtcggcat gcaccgccac
     3241 tgggtcgacc cgacggagcc gttcgccgcc gcggtgctgg cgcctgccca cggcgcgctg
     3301 atcacctcgg cgaccttgcg cgacggcacc ggcgatgtcg aggccgactg ggccgcggcc
     3361 gaggcgcgca ccggcgccgc gcatctcgcc gagcccgcgc tgcgtgccgc cgtgccctcg
     3421 ccgttcgact acgccgccca gacccgcgcc ctggtggtca ccgacgtcga ccgcgacgac
     3481 cccgacgtga ctgcggccgc ctatcgcgag ctgttcctgg ctgcgggcgg cggcgcgctt
     3541 ggcttgttca ccgcgatctg gcggctgcgc cggattcatg cgcgcatcgc cggcccgctc
     3601 gaggaggcgg gcttggtgtt gctcgcccag catgtcgacg cgatggacac gggcacgctc
     3661 gtcgacatct tccgtgccga gcgcaacgcg tgcctgttgg gcaccgacgc gctccgcgac
     3721 ggcgtcgacg tgccgggcga ggcgctgcgc ctgatcgtgt tcgaccgcgt gccctggccg
     3781 cgcccggaca tcctgcacaa ggcgcggcgc gcgcgcttcg gcggccgcgc ctacgacgac
     3841 atgatcaccc gcctgcgctt gcgccaggcc ttcggccgcc tggtgcggcg cgccgacgac
     3901 tacggcgtgt tcgtgatgct cgacgccgcc accccctcgc gcctctacgg cgccttcccc
     3961 gacggcgtcg aggtcacgcg cgccggcctc gccgaggccg tccaaaccac ccgcaccttc
     4021 ctcgccgaac acgcgcgcac gcgtccgccc agcgtcgcgt gagttcgtag gtacatcatt
     4081 gatggtcaaa tgcgaacgaa gatgccgacg gggtacgagc ttcctctctg tcgtacggat
     4141 gtcccctttt
//
LOCUS       DASWHU010000054         5122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1872235, whole genome shotgun sequence.
ACCESSION   DASWHU010000054 DASWHU010000000
VERSION     DASWHU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872235"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..86
                     /locus_tag="VGB88_01995"
     CDS             <1..86
                     /locus_tag="VGB88_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9646239.1"
                     /translation="GAVRAALWTRGRAPGLYGMREVLGLAG"
     gene            587..2023
                     /locus_tag="VGB88_02000"
     CDS             587..2023
                     /locus_tag="VGB88_02000"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HEX9646240.1"
                     /translation="MKSMVGLEIQSSPQGAPAAVDRQPQETLVAAVLPSLESGQPAQI
                     DITLAADLLAGQAAKELSLVDFDPGVLDEAFPIPEAAIVETGPASTQDGASGPDVVYS
                     GGTSFEPSTPDEELVGEPRLAVEGLSRPDVGEHDRRDRLDDGNVRRGGPDSAAITVTA
                     AGVNQANGAIADPADAPYARDDAAPTAVDAVVTIDVLSNDGDPNGDPVAVAAVTQGAS
                     GTVVINPDDTVSYTPNGGFSGVDSFTYTADDGNGGTDSATATVYVGIATVGGSGKDTI
                     SGTNGDDVIFGQEGNDRLSGGKGDDTLYGGDGNDVLNGQNGDDEIFGNAGNDTLNGNQ
                     GDDYLDGGAGSDSLSAGNGDDILVWDFADRSINGGAGSDTLLVDHGDADIAGFAGTIR
                     RIEQVDLDSDDKANTLTLSAQDVLDMSDTNVVKIFGDSNDAVEAGMGWTDGGIDGNGY
                     QIFTQTVGGSLATLLVDPDIAVNPDVLA"
     gene            complement(2047..2931)
                     /locus_tag="VGB88_02005"
     CDS             complement(2047..2931)
                     /locus_tag="VGB88_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9646241.1"
                     /translation="MGARELALLAALALMWAASFTFIKIAVATVPPATIVAGRLAVAA
                     VLLWAYLALTGRRLPRPGRAWRLYGVLAVTGNTLPFLLIAWAETRVDSGLAAILIGTV
                     PVITVLLAHRLSPDEPLGAHTWAGVALGLAALAVLAGPHALAGLGGTLLAQVALVGAA
                     ASYALNGLLVRRIRADVVSASAAVLVASAALALPISLALDRPWQLAPSWAAVWAVLAQ
                     GAFSTALATIAFFHLVTVAGAAATSFVNYLIPAVAVALGVVWLGERPGWPELAALALI
                     LAGLALVNRRRASRPPRT"
     gene            complement(2967..3470)
                     /locus_tag="VGB88_02010"
     CDS             complement(2967..3470)
                     /locus_tag="VGB88_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX9646242.1"
                     /translation="MADAPAAARNELLFDAVLHPHRSLSRGGFMALMAALGAVSFTAG
                     MVFLLAGAWPVFGFFGLDVALVWLAFRLNYRSGRLVERVRLTCQALEVERIAASGRAS
                     RWRFQPYWLRVVLEDRGEHHSRLALRSHGKELVIGAFLSPPERASLCAALTAALARCR
                     CLPAAIG"
     gene            3798..4472
                     /gene="nth"
                     /locus_tag="VGB88_02015"
     CDS             3798..4472
                     /gene="nth"
                     /locus_tag="VGB88_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9646243.1"
                     /translation="MRKAEIEQMFERFAAADPAPETELDYLDPYSLLIAVVLSAQATD
                     TSVNKATPALFALADSPAKMVALGEERVRELIKTIGLYRTKAKNVVALSRALVERHGG
                     AVPRDREALEALPGVGRKTANVVLNAAFGEPTIAVDTHLFRLANRTGLAPGKTPRAVE
                     DKLMEVVPERFRRHAHHWLILHGRYVCTARRPNCPECPIRDLCGYQAKTPGVAAPARR
                     RQRSTT"
     gene            complement(4412..>5122)
                     /locus_tag="VGB88_02020"
     CDS             complement(4412..>5122)
                     /locus_tag="VGB88_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX9646244.1"
                     /translation="AGMLDDLGKSIGQLFEPELAAVLWKILGATILVFAGLWLGVGWL
                     LGSVTVADIGWLDRLAGWLVSLLGGVLAVVLTLVLFAPVATMVAALFQDQVADAVEAR
                     HYPDLAKAPGQPIGRQIAAGLRLLIWTVVVNLLCLPLYLLLPGANIVLFFAVNGWLLS
                     REYFEAVALRRLERRPARAFRRRHRLSLWLAGVALAVVFWVPVVNLVAPVLGTALFVH
                     VVERCRRRAGAATPGVLA"
BASE COUNT          893 a         1825 c         1785 g          619 t
ORIGIN      
        1 ccggcgcggt gcgcgcggcg ctgtggacca gggggcgcgc gcccgggctc tacggcatgc
       61 gcgaggtgct cggcctcgcc ggttaggccg tgcccggaac ctgaaggccc gcgtccggcc
      121 tcgcgacagg cctgcggagg atcggccggg aaccacccga gggaaagcca cccggcgagg
      181 agtggcgggt cggcttgccg ttgcctccgg ttcgtcgcgg gtcatggccg cgcggcgcag
      241 ccgccgttgg acggcctcct cacctggtgg ctccaagact ccagcctggc acatcgatgc
      301 cgtcaggggg ccgtccaccc ggcattgctc gaccggaaca aatggcccat cggcgcgtca
      361 ccgatcgacc gtggcaacgg ccgaaaccgg tcatccgcat ccaatcaagt ctgcctgtcc
      421 gtccgatcgc atcgtgatga tcgtcacaat gactgccggt tctgagggga attgcttcgt
      481 tgatacggcg cttggttgcc gccgctctaa ataggttact gggtgctagg agattcccta
      541 gccgtcttag gacgatccca acatcataac cgtaaggagc ggtgagatga aaagcatggt
      601 cgggctcgaa atccagagtt ctccgcaggg cgcacccgcc gcggtcgacc ggcagccgca
      661 ggagacgctc gtcgcggctg ttcttccgtc acttgaatcc gggcaacctg ctcaaatcga
      721 tatcacgttg gcggccgatc ttcttgcggg ccaggcggcg aaggagctgt cgctggtaga
      781 cttcgatccc ggagtcctgg acgaagcatt tcccattccc gaggcggcga tcgttgagac
      841 gggaccggcc tcgactcaag acggcgcctc gggaccggat gtcgtctaca gcggcggtac
      901 cagcttcgag ccttcgacgc cggacgagga gctcgtcggc gagcctcggt tggcggtcga
      961 aggcctgtca cggccggacg tcggtgagca cgacaggcgc gaccgtcttg acgacggcaa
     1021 tgtcaggcgc ggcggccccg acagtgccgc catcaccgtc accgccgccg gtgtcaacca
     1081 ggcgaacggc gcaatagccg accccgccga cgcgccgtac gcccgagacg atgcggcgcc
     1141 caccgccgtc gatgcggtcg tcaccattga tgtgctgagc aacgacggcg acccgaacgg
     1201 cgatcccgtg gccgtggcgg ctgtgactca gggcgcaagc gggaccgtcg tcatcaaccc
     1261 cgacgacacg gtcagctata cgccgaacgg cggcttcagc ggcgtcgaca gcttcaccta
     1321 cacggccgac gacggcaacg gcggcacgga tagcgcgacg gcgacggtct atgtcgggat
     1381 cgcgaccgtc ggcgggtcag gcaaggacac gatcagcggc accaatggcg atgacgtgat
     1441 cttcggccaa gaaggcaatg acagactgtc gggcgggaaa ggcgacgaca cgttgtacgg
     1501 cggcgacggc aacgacgtgc tgaacggcca aaatggcgac gacgagatct tcggcaacgc
     1561 tggcaatgac accctcaacg gcaaccaggg cgacgactac ctcgacggcg gcgcggggag
     1621 cgacagcctg agcgccggca acggcgacga catcttggtc tgggacttcg ccgaccgctc
     1681 catcaacggc ggtgccggta gcgacacgtt gctggtcgac cacggcgacg ccgacatagc
     1741 cggcttcgcg ggcacgatcc ggcgcatcga acaggtcgac ctcgactcgg acgacaaggc
     1801 caatacgctc acgttaagcg cacaagatgt gctcgacatg tccgatacca acgtcgtcaa
     1861 gatctttggc gacagcaacg atgcggtcga ggcggggatg gggtggaccg acggcggcat
     1921 cgacggcaac ggctaccaga tcttcaccca gaccgtcggc ggcagcttgg cgacattgct
     1981 tgtcgacccc gacatcgcgg ttaacccgga tgtcttggcg taggccgagg ggcgcgcgcg
     2041 cctgacctat gtccgcggcg gccgacttgc ccggcggcgg ttgaccaaag cgaggccggc
     2101 gaggatcagg gcaagcgccg cgagctcggg ccagcccggc cgctcgccga gccacaccac
     2161 cccgagcgcc accgccaccg ccgggatcag gtaattgacg aacgatgtcg ccgccgcgcc
     2221 cgccaccgtg accaggtgga agaaggcgat ggtggcgagc gcggtgctga aggccccctg
     2281 ggcgagcacc gcccacaccg ccgcccagct cggcgcgagc tgccacggcc ggtcgagggc
     2341 gagcgagatc ggcagcgcga gcgccgccga ggcgaccagc acggcggcgg acgccgacac
     2401 gacgtcggcg cggatccgcc gcaccagcag accgttcagc gcgtaggacg ccgccgcgcc
     2461 gaccagcgcc acttgggcca acagcgtgcc gccgaggccg gcgagcgcat gcggcccggc
     2521 gagcaccgcc agcgccgcga ggccgagcgc gactcccgcc caggtgtgcg cgccgagcgg
     2581 ctcgtccggg ctcaggcgat gggccagcag caccgtgatc accggcacgg tgccgatcag
     2641 gatcgccgcc agccccgaat cgacgcgcgt ctccgcccac gcgatcagca ggaacggcag
     2701 cgtgttgccg gtcaccgcga gcacgccgta cagccgccag gcgcgtcccg ggcgcggcag
     2761 ccggcgcccg gtcagggcga gatacgccca cagcagcacg gccgcgaccg cgagcctgcc
     2821 ggcgacgatg gtcgcgggcg gcacggtggc gaccgcgatc ttgatgaagg tgaacgacgc
     2881 cgcccacatc agcgccagcg ccgccagcaa ggcgagctcg cgcgcgccca tccgccgcag
     2941 tgcgccgggg agggcggcgg gccggctcag ccgatcgcgg cgggcaggca gcggcagcgg
     3001 gcgagcgcgg cggtgagcgc ggcgcacagg ctcgcccgct cgggcgggct caggaacgcg
     3061 ccgatcacca gctccttccc gtgcgaacgc aatgcgaggc ggctgtggtg ctcgccccgg
     3121 tcctcgagca cgaccctcag ccagtagggc tggaaccgcc agcggctggc ccgcccggag
     3181 gccgcgatcc gctcgacctc gagcgcctgg caggtcagcc gcacgcgctc gaccagccgg
     3241 cccgagcggt agttgaggcg gaaggcgagc cagaccagcg ccacgtccag cccgaagaag
     3301 ccgaacaccg gccacgcgcc ggcgagcagg aacaccatcc cggcggtgaa gctgaccgcg
     3361 cccagcgccg ccatcagggc catgaaaccg ccccggctca acgaccggtg cgggtgcagg
     3421 acggcgtcga acaacagctc gttgcgggcg gcggcgggag catcggccat ggccggatga
     3481 tgggcacgct gcgaccgctt gtcaaaatcg cccgcggccg cgccggccac cgcctcggtg
     3541 ttgcgctcac gcggagtcgc ggaggacgcg gagacctcgg acccggctct catggctcac
     3601 ccccgcgcaa gcgggatcaa gtaacatatt gaaaagcccg acttccgcgt tcgcggcagt
     3661 gacgacgcag gtagcgcggc ggaggtgggt gccctccgcg tcctctgcgc ctccgcgtgg
     3721 gcacgaccta tcccgccgcc ttgacgcacg ccttcggcgg tgtctaatcg gcgacgccgg
     3781 cgcgccgggg ggaggcgatg aggaaggccg agatcgagca gatgttcgag cgcttcgccg
     3841 ccgccgaccc ggcgccggag accgagctcg actacctcga cccctacagc ttgctgatcg
     3901 cggtggtgct gtcggcgcag gcgaccgaca cgagcgtcaa caaggcgacc ccggcgctgt
     3961 tcgcgctcgc cgattcgccc gccaagatgg tcgcgctggg cgaggagcgg gtgcgcgagc
     4021 tgatcaagac catcggcctc taccgcacca aggcgaagaa cgtggtcgcg ctcagccggg
     4081 cgctggtcga gcgccacggc ggcgcggtgc cgcgcgatcg cgaggcgctc gaggcgctgc
     4141 ccggggtcgg gcgcaagact gccaacgtgg tcctcaacgc ggccttcggc gagcccacca
     4201 tcgccgtcga cacccatctg ttccgcctcg ccaaccgcac cgggctcgcg cccggcaaga
     4261 ccccgcgcgc ggtcgaggac aagctgatgg aggtggtgcc cgagcgcttc cgccgccacg
     4321 cccatcactg gctgatcctg cacggccgct acgtgtgcac ggcgcgccgg ccgaactgcc
     4381 ccgaatgccc gatccgcgac ctgtgcggct atcaggccaa gaccccgggg gtcgccgcgc
     4441 cggcgcgccg ccggcagcgc tcgaccacgt gaacgaacag ggcggtgccg agcaccggcg
     4501 cgaccaggtt caccaccggc acccagaaca cgaccgcgag cgcgacgccg gcgagccaca
     4561 ggctcaagcg atggcggcgg cggaacgccc gcgccgggcg ccgctcgaga cggcgcagcg
     4621 ccaccgcctc gaaatactcg cggctcagca gccagccgtt gaccgcgaag aacaggacga
     4681 tgttggcccc cggcaacagc aagtacagcg gcaggcacag caggttgacc accacggtcc
     4741 agatcagcag ccgcagcccc gcggcgatct gccggccgat cggttgcccc ggcgccttgg
     4801 ccaggtcggg gtagtgccgc gcctcgaccg catcggcgac ctggtcctgg aacagcgccg
     4861 ccaccatggt cgccacgggc gcgaacagca ccagggtcag caccacggcg agcacgccgc
     4921 ccaggagact gacgagccac ccggccagtc ggtcgagcca gccgatgtcg gccacggtga
     4981 cgctgccgag cagccagccg acaccgagcc acaggccggc gaacaccagg atcgtcgcgc
     5041 cgaggatctt ccacagcacg gccgcgagct caggctcgaa gagctggccg atcgacttgc
     5101 cgagatcgtc gagcattccg gc
//
LOCUS       DASWHU010000055         9103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2240712, whole genome shotgun sequence.
ACCESSION   DASWHU010000055 DASWHU010000000
VERSION     DASWHU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240712"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1857)
                     /gene="groL"
                     /locus_tag="VGB88_02025"
     CDS             complement(220..1857)
                     /gene="groL"
                     /locus_tag="VGB88_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567757.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9646245.1"
                     /translation="MAAKEVKFGMDARARMLRGIDVLADAVKVTLGPKGRTVVLDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMVREVASKTNDIAGDGTTTATVLAQAIVRE
                     GLKSVAAGMNPMDLKRGIDLAVEAVVDNLAKQAKTVKTNDEIAQVGTISANGDREVGR
                     IIASAMERVGNEGVITVEEAKGLETELEVVEGMQFDRGYLSPYFITNAEKMQCELEDA
                     YILLHEKKLANLQALLPLLEAVVQSSRPLLVIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDMAVLTGGQVISEDLGIKLESVTLDMLGRAKRISITKDDTTVID
                     GAGKRAAIEGRCTQIRQQIDDTTSDYDREKLQERLAKLAGGVAVIKVGGATEVAVKER
                     KDKVDDALHATRAAVEEGIVIGGGVALLYAAKALDKIKTENHDQQVGIDIVRKALQTP
                     VRQIAENAGVDGSVVVGKLLDQKKETWGFDAQLETYVDMFKAGIVDPAKVVRIALQDA
                     ASVAGLLVTTEAMVGEKPERKSGGGAPNMPDMGGMDF"
     gene            complement(1892..2212)
                     /gene="groES"
                     /locus_tag="VGB88_02030"
     CDS             complement(1892..2212)
                     /gene="groES"
                     /locus_tag="VGB88_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9646246.1"
                     /translation="MKFRPLHDRVVVRRLEEDERTTGGIIIPDTAKEKPSQGEVVAVG
                     PGARDESGEIVALDVKKGDRVLFGKWSGTEVKIDGQELLIMKESDIMGVIEETVRKAS
                     RKAA"
     gene            2480..2758
                     /locus_tag="VGB88_02035"
     CDS             2480..2758
                     /locus_tag="VGB88_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEX9646247.1"
                     /translation="MTVHAHRVTELQLRGYRLVTAEILYRMPDHPALLQTFVWQDYDI
                     APRFPVLNQFLDFWERNLDGPLHSVRVASTELIGPSRWRRAGGVFTIQ"
     gene            2778..3581
                     /locus_tag="VGB88_02040"
     CDS             2778..3581
                     /locus_tag="VGB88_02040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9646248.1"
                     /translation="MTAERDLAAGSPERFGYAWHRFSLPSAAQEEQFRRWTSLIDPAT
                     GWRGMTFLDAGCGAGRNAYWAMSWGAAGGVAVDVDERALAAARGNLAAFPSVEVRHRS
                     LYDLDLDNAVDIAFSIGVVHHLSDPDRALAALVRAARPGGRVLVWLYGVENMELYLKL
                     FAPVRRALFSRLPMPALAALAHMPCAALWLALRLGLGRIEYVRLLRGFPYRHLHAIVL
                     DQMLPRIATHYTRARAEALLARAGLTDVRSCWVNQMSWQVVGTKPAGSS"
     gene            3659..4597
                     /locus_tag="VGB88_02045"
     CDS             3659..4597
                     /locus_tag="VGB88_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646249.1"
                     /translation="MGEAVRAAVAEAPVADTRYTLRARYFDSATAFNLHLPPVPAHSF
                     TDEARAALDAATGTALIALDLSQAMALDYPATTPLVLARYGRIRAGETLVLEAKATVE
                     IYYVIAGAGRSVKGADSIAWAEGDVFALPGGAPSEHRAEGGDAVLWIVTNEPELAFEG
                     AEAPAPARARVEAVHFPAAEIDRRLAVVERKLAGTRTPGLAVVFSSAAQESRRNISPS
                     LTVAMNQLQPGGSQIPHRHNSVAVSLPISFARCYSMVDGVRYDWQPYATTVTPPDSVH
                     SHHNDGERHGRWLIVQDGGLHYHCRTMGFAYADPAP"
     gene            4814..6685
                     /locus_tag="VGB88_02050"
     CDS             4814..6685
                     /locus_tag="VGB88_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX9646250.1"
                     /translation="MIEAIPLAILIGAGLVVISILSSFISFRVGAPLLLVFLGVGLAA
                     GEDGIGGIEFENARAAYFVGTVALAIILFDSGIGTRVQIFRVAVAPAIVLATVGVLLT
                     AVLVAVPVRVLLGFDWLQAFLLGAIVSSTDAAAVFFLLRVGGIQVRERVRATLEIESG
                     SNDPMAIFLTLTLVELVASGATGLRDISWDFLAAFGAEMGLGLLIGLGAGYLIVLGLN
                     RVRLEPVLFSIVVLGLALCVFAVTSLLGGSGFLAAYVVGVAMGNLPLRGVIALRRFHE
                     GFTWLAQITMFLVLGLLATPSEFLAIGWQAVAIGLVLMLVARPIAVWACLLPFGFQRN
                     ETTFAAWIGLRGAVSILLAILPIIGGLEHGQMMFNAAFIIVLTSLLIQGWTIRPMAKW
                     LGLVVPPHIGPVEKVELELPGDARHELLSYRVAPESPVAKGERIPRWARPSLVVRDGR
                     SMRIHDAGRPQPGDYVYIFAVPRFIPLLDRLFASPVELTDKDLEYFGEFTVDPAHTIG
                     EVARTYGFEVAAEDAEVPIGDFVRRRLGTPLGRGDRLPLPPIELIVREADDDGGPLSI
                     GLSVEPGPASIPRLPLFQNYREIMAGVRAWLKRRSAARLNPSRSPRSSRRVRRRRPP"
     gene            complement(6627..7154)
                     /locus_tag="VGB88_02055"
     CDS             complement(6627..7154)
                     /locus_tag="VGB88_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646251.1"
                     /translation="MASQEILLVSYSLGGHTEAAARAIAGRLGSAHEAIDAPAIGRGL
                     AGVVRALWSALRRTRPPLAPARHDPASYSLVVVAAPIWAGRPATPALSYLATHAAALP
                     AVAFVLTHGGSDPTRAFEMLAAAAGKAPLARLALSEADRKSGADAAKVEDFVAELTAA
                     AGGARGASCEDSGSG"
     gene            complement(7158..8900)
                     /gene="nirK"
                     /locus_tag="VGB88_02060"
     CDS             complement(7158..8900)
                     /gene="nirK"
                     /locus_tag="VGB88_02060"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEX9646252.1"
                     /translation="MMFSFRSLAGIGLLLALAFGAISAAATEDAEDGARVFRKCAACH
                     SLEAGKHKIGPSLAGVMGRAAAAAGGFRYSDALAQSGLTWDAATLEAFLAGPMKLVPG
                     TRMVFPGLKKAEDRAAVIAFLSAGAPAAAAAAPAAPLPAAGAESSALPAGYIPDVRYT
                     LETGIAQGRMVYIGFGGDIDGIVNPDLSAGEGDVVQITLINGEGAEHDIVIAEHGVAS
                     QHVLDKGAATTVAFRARAAGTTPYFCSLPGHREAGMEGRFTVTTAVAEAAPVAASIVM
                     DPAAVPAPIGDRPPQRVRVELNAVELDGELADGTTYPYWTFNGTVPGPFIRVRVGDTV
                     EVVLANDADSSMIHSVDFHATTGPGGGAAHLQVDPGDSKRLDFKALVPGIFVYHCATP
                     MVAHHIANGMYGLILVEPEGGLPPVDKEFYVMQGEIYTHAPFGTPGSQEFSVEALLNE
                     TPEYFVFNGAVGALTTQRPLRAQVGETVRLFVGVGGPNFISSFHVIGEMFDRLYNWGD
                     LVSPPARGVQTVAVPPGGAVVTEFALEVPGRYILVDHALSRLERGLVGFLYAEGEPNP
                     EVYKTYDGSPGGLD"
BASE COUNT         1240 a         3239 c         3141 g         1483 t
ORIGIN      
        1 tgccgttgcg cccgaccagg cacagccggt cgcgcgcgcc gagcaccagg tccgcgtccg
       61 cgaacacggg ctcaggcccg aagcgcaccg caaccgacct gagcgttaag atcggggctg
      121 ccatcgccgc atcctagcgc cccgcggcgg gccgaaaaat atggcgggcc gcatgacgcg
      181 gcccgccggt cgtatgggac gtcgcccgcg cggcgcggat cagaagtcca tgccgcccat
      241 gtcgggcatg ttcggcgcgc cgccgcctga cttcctctcc ggcttctcgc cgaccatcgc
      301 ctcggtggta accagcagcc ccgccaccga cgcggcgtcc tggagcgcga tgcgcaccac
      361 cttggccggg tcgacgatgc cggccttgaa catgtcgaca taggtctcga gctgggcgtc
      421 gaagccccag gtctccttct tctggtccaa gagcttgccg accaccaccg agccgtcgac
      481 gccggcgttc tcggcgatct ggcggaccgg ggtctgcaac gccttgcgca cgatgtctat
      541 gccgacctgc tggtcgtggt tctcggtctt gatcttatcg agcgccttgg cggcataaag
      601 cagcgccacg ccgccgccga tcacgatgcc ctcctcgacc gcggcgcggg tggcatgcag
      661 ggcgtcgtcg accttgtcct tgcgctcctt gaccgcgacc tcggtggcgc cgccgacctt
      721 gatcacggca acgccgccgg cgagcttggc gaggcgctcc tgcagcttct cgcggtcgta
      781 gtcggaggtg gtgtcgtcga tctgctggcg gatctgggtg cagcggccct cgatcgcggc
      841 gcgcttgccg gcgccgtcga tgacggtggt gtcgtccttg gtgatgctga tccgcttggc
      901 ccggccgagc atgtcgaggg tgaccgactc gagcttgatg ccgaggtcct ccgagatcac
      961 ctggccgccg gtcagcaccg ccatgtcctc gagcatcgcc ttgcggcggt cgccgaagcc
     1021 cggcgccttg accgcggcga ccttcaagcc gccgcgcagc ttgttgacca ccagggtggc
     1081 gagcgcctcg ccctcgacgt cctcggcgat caccaacagc ggccggctcg actgcaccac
     1141 ggcctcgagc agcggcagca gcgcctggag gttggcgagc ttcttctcat gcagcaggat
     1201 gtaggcgtcc tcgagctcgc actgcatctt ctcggcgttg gtgatgaagt agggcgacag
     1261 gtagccgcgg tcgaactgca tgccctcgac cacctcgagc tcggtctcca ggcccttggc
     1321 ctcctcgacc gtgatcacgc cctcgttgcc gacccgctcc atggcgctgg cgatgatcct
     1381 gcccacctcg cggtcgccgt tggcggagat ggtgccgact tgggcgatct cgtcattggt
     1441 cttcaccgtc ttggcctgct tggcgaggtt gtcgaccacc gcctcgaccg ccaggtcgat
     1501 gccgcgcttg aggtccatcg ggttcatgcc cgcggcgacc gacttgagcc cctcgcggac
     1561 gatcgcctgg gcgagcacgg tggcggtggt ggtgccgtcg ccggcgatgt cgttggtctt
     1621 cgatgccacc tcgcgcacca tctgcgcgcc catgttctcg aacttgtcgg cgagctcgat
     1681 ctccttggcg acggtcaccc cgtccttggt gatccgcggc gcgccgaacg acttgtcgag
     1741 caccacggtg cggcccttcg gcccgagcgt caccttgacc gcgtccgcga gcacgtcgat
     1801 gcctctcagc atccgcgcgc gggcgtccat gccgaactta acttcctttg cagccatttc
     1861 agatcactcc cattggattg cctcggcgcg cctaggcggc cttcctgctg gctttgcgga
     1921 cggtttcctc gatcacgccc atgatgtcgg actccttcat gatcaacagc tcctggccgt
     1981 cgatcttgac ctcggtgccg gaccacttgc cgaacaggac ccggtcgccc ttcttgacat
     2041 cgagggcgac gatctcgccg ctctcgtcgc gggcgcccgg cccgaccgcc accacctcgc
     2101 cctggctcgg cttctccttg gcggtatccg ggatgatgat gccgcccgtg gtccgctcgt
     2161 cttcctcgag cctgcggacc acgacacggt cgtgcaaagg acggaatttc atcgcatttg
     2221 tccccttcgc gatagaggtt ggctgaacac tgaccacgaa ccccctaagc actcgacggt
     2281 atcgagtgct aacaggccca tggcgcatgt ggcccaagcc cgcgcgacag tcaagggggc
     2341 ttggccgaat ttgtcagcaa tccctctgtg cggctgctaa aggattgttt ttattgagtt
     2401 cttagacgat ggggcttccc ctaatgcgcg cgacgtgaga tgatgaaaca tgtgacccag
     2461 gccaaccgtg cgtcctgtga tgactgtcca cgcccaccga gtgaccgagc ttcagctcag
     2521 gggataccgg ctggtcaccg ccgagatcct ctaccggatg ccggaccatc cggcgctgct
     2581 ccagaccttc gtttggcagg actacgacat cgcgccccgc tttccggtgc tcaaccagtt
     2641 tctcgacttc tgggagcgca acctggacgg tcccctgcac tcggtgcgcg tcgcctcgac
     2701 cgagctgatc gggccgagcc gctggcgccg cgccgggggc gtctttacca tacagtaggc
     2761 gcggggcgcg ggcggcgatg accgccgagc gcgacctcgc cgccggctcg ccggagcggt
     2821 tcggctacgc ctggcaccgc ttcagcctgc cgagcgccgc ccaggaggag cagttccggc
     2881 gctggaccag cctgatcgac cccgccaccg gctggcgcgg gatgaccttc ctcgacgccg
     2941 gctgcggggc ggggcgcaat gcctactggg cgatgagctg gggcgccgcg ggcggcgttg
     3001 cggtcgacgt ggacgagcgc gccctcgccg cggcgcgggg caacctggcg gcgttcccgt
     3061 cggtcgaggt ccgccaccgc agcctctacg acctcgacct cgacaatgcg gtcgacatcg
     3121 cgttctcgat cggcgtcgtc caccatctgt ccgaccccga ccgggcgctc gccgcgctgg
     3181 tgcgcgccgc caggccgggc gggcgggtgc tggtgtggct gtacggggtc gagaacatgg
     3241 agctctatct caagctgttc gcgccggtga ggcgggcgct gttcagccgg ctgccgatgc
     3301 cggccctcgc cgcgctcgcc cacatgccgt gtgcggcgct gtggctcgcg ctcaggctcg
     3361 gcctgggccg gatcgagtat gtccgcctct tgcgcggctt cccctaccgc catctgcacg
     3421 cgatcgtgct cgaccagatg ctgccgcgca tcgccaccca ttacacccgt gcccgggccg
     3481 aggcgctact cgcgcgcgcc gggctcaccg acgtccgctc gtgctgggtc aaccagatgt
     3541 cctggcaggt ggtcggcacc aagcccgcgg ggtcgagttg accgccgccc gaggccgctc
     3601 ctacactcgc cggagcggtc gccggagcaa ccgcggcatg gtccgggagg ggatacgaat
     3661 gggcgaggca gtgcgggcgg ccgtggcgga ggcgccggtc gcggacacgc gttacacgct
     3721 gcgcgcgcgc tatttcgatt cggccaccgc gttcaacctc catttgccgc cggtgcccgc
     3781 gcacagcttt accgacgagg cccgcgccgc gctcgatgcc gccaccggca cagcgctgat
     3841 cgcgctcgac ctgagccagg ccatggcgct cgactacccg gcgacgacgc cgctggtgct
     3901 ggcgcgctac ggccgcatcc gcgccggcga gacactggtg ctcgaggcga aggcgacggt
     3961 cgagatctac tacgtcatcg ccggcgccgg gcgcagcgtc aagggcgccg acagcatcgc
     4021 ttgggccgag ggcgacgtgt tcgcgctgcc cggcggtgcg ccaagcgagc atcgcgccga
     4081 gggcggcgat gcggtgctgt ggatcgtcac caacgagccc gagctcgcct tcgagggcgc
     4141 cgaggcgccg gccccggcgc gcgcgcgcgt cgaggcggtg cacttcccgg cggccgagat
     4201 cgaccgccgg ctcgcggtcg tcgagcgcaa gctcgccggc acgcgcaccc ccggcctcgc
     4261 ggtggtattc tcgagcgccg cgcaggaatc gcggcgcaac atctcgccgt cgctgaccgt
     4321 ggcgatgaac cagctgcagc cgggcggcag ccagatcccg caccgccaca actcggtcgc
     4381 ggtgtcgctg ccgatctcgt tcgcgcgctg ctactcgatg gtcgacgggg tgcgctatga
     4441 ctggcagccc tatgcgacca cggtcacccc gcccgacagc gtccactcgc accacaatga
     4501 cggcgagcgc cacggccgct ggctgatcgt ccaggacggc ggcctgcact accactgccg
     4561 caccatgggc ttcgcctacg ccgatccggc gccgtaaccc gcggcgagcg cgggcgcgcc
     4621 ggctcgccaa tagatcggga aagggaagca tgctaaggag cttggcgccg atgctggcgc
     4681 ccggcggcgg ccggatgcgg tcggcgccca ggacatagcg gagaatccgc ccgacctccc
     4741 tcgccgccga ccgggacgcc ggatatttcg gccaccgacg agcctctccc cggcagcgtg
     4801 aaggaacatc tctatgatcg aggcgatccc gcttgcgatc ctcatcggcg cggggctggt
     4861 ggtcatctcg atcctttcca gcttcatctc gttccgcgtc ggcgcgccgc tgctgctggt
     4921 gttcctcggc gtcgggctcg cggcgggcga ggacggcatc ggcgggatcg agttcgagaa
     4981 cgcccgggcc gcctacttcg tcggcacggt cgcgctcgcc atcatcctgt tcgacagcgg
     5041 catcggcacg cgcgtccaga tcttccgggt agcggtggcg ccggcgatcg tgctcgccac
     5101 cgtgggcgtg ctgctcaccg cggtactggt cgcggtgccc gtgcgcgtcc tgctcgggtt
     5161 cgactggctg caggcgttcc tgctcggcgc gatcgtcagc tcgaccgacg cggcggcggt
     5221 gttctttctg ttgcgcgtcg gcggcatcca ggtccgcgag cgggtgcgcg ccacgctcga
     5281 gatcgaatcg ggatcgaacg atccgatggc gatcttcctg accttgaccc tggtcgagct
     5341 ggtcgccagc ggggcgactg ggctgcgcga catctcctgg gacttcctcg ccgcgttcgg
     5401 tgccgagatg ggtctcggcc tgctaatcgg gctcggcgcc ggctatctga tcgtgctcgg
     5461 cctcaaccgg gtcaggctcg agccggtgct gttctcgatc gtcgtcctcg ggctcgcgct
     5521 atgcgtgttc gccgtgacct cgctgctcgg cggcagtggc ttcctcgccg cctacgtggt
     5581 cggcgtggcg atgggaaatc tgccgctgag gggggtgatc gcgctcaggc gcttccatga
     5641 aggattcacc tggctcgcgc agatcaccat gttcctggtg ctcgggctgc tggcgacgcc
     5701 gtccgagttc ctggcgatcg gctggcaggc ggtggcgatc ggactcgtct tgatgctggt
     5761 cgcgcggccg atcgcggtct gggcctgcct gctgccgttc ggcttccagc gcaacgagac
     5821 cacgttcgcc gcctggatcg gcctgcgcgg cgcagtgtcg atcctgcttg cgatcctgcc
     5881 gatcatcggc ggcctcgagc acggccagat gatgttcaac gccgccttca tcatcgtgct
     5941 cacctcgctc ctgatccagg gctggacgat ccggccgatg gccaagtggc tcggcctggt
     6001 cgtgccgccc cacatcgggc cggtggagaa ggtggaactc gagctgcccg gcgacgcccg
     6061 ccacgagctg ctcagctacc gggtcgcgcc ggagagcccg gtcgcgaagg gcgagcgcat
     6121 cccgcgctgg gcgaggccgt ccctggtcgt gcgcgacggc cggtcgatgc gcatccacga
     6181 cgccgggcgc ccgcagccgg gggattacgt ttacatcttc gctgtgccgc gcttcatccc
     6241 gctgctcgac cggcttttcg cgagccccgt ggagctgacc gacaaggacc tggagtattt
     6301 cggcgagttc accgtcgatc cggcgcatac catcggcgag gtcgcccgga cctacggctt
     6361 cgaggtcgcc gccgaggacg ccgaggtgcc gatcggcgac ttcgtcagga ggcggctcgg
     6421 cacgccgctc gggcgcggcg accggctgcc cctgccgccg atcgagctga tcgtgcgcga
     6481 ggccgacgac gacggcgggc cgctgtcgat cgggctctcc gtggagccag gcccggcgtc
     6541 gatccccagg ctcccgctgt tccagaatta ccgggagatc atggccgggg ttcgcgcttg
     6601 gttgaagcgg cgttcggcgg cgcgcctcaa cccgagccgg agtcctcgca gctcgcgccg
     6661 cgtgcgccgc cggcggccgc cgtgagctcg gccacgaaat cctcgacctt ggcggcgtcg
     6721 gcgccgctct tgcggtcggc ttcggagagc gcgaggcgcg cgagcggcgc cttgccggcg
     6781 gcggcggcga gcatctcgaa cgcgcgcgtc ggatcgctgc cgccgtgggt gaggacgaag
     6841 gcgaccgccg gcagggcggc ggcgtgggtc gcgagatagc tcagcgccgg tgtcgccggc
     6901 cgcccggccc agatcggcgc ggcgaccacc accaggctat agctcgccgg gtcatggcgg
     6961 gcaggcgcga gcggcgggcg ggtgcgccgc agcgcgctcc acagcgcgcg caccacgccg
     7021 gcgagcccgc ggccgatcgc cggcgcatcg atcgcctcgt gcgcggagcc gaggcgcccg
     7081 gcgatggcgc gcgccgccgc ctcggtatgg ccgccgagcg agtaggacac cagcaggatc
     7141 tcttggctcg ccatgcgcta gtcgagcccg ccgggggagc cgtcgtaggt cttgtagacc
     7201 tccgggttgg gctcgccctc ggcatagagg aagccgacca gcccgcgctc gagacggctc
     7261 agcgcgtggt cgaccaggat gtagcggccg gggacctcga gcgcgaactc cgtgaccacg
     7321 gcgcccccgg gcgggacggc gaccgtctgc acgccccgcg ccggcgggct gaccaggtcg
     7381 ccccagttgt agagccggtc gaacatctcg ccgatgacgt ggaacgacga gatgaagttc
     7441 ggcccgccga cgccgacgaa cagccgcacc gtctcgccga cctgggcgcg cagcggacgc
     7501 tgggtcgtca acgcgccgac cgcgccgttg aagacgaagt actcgggcgt ctcgttgagc
     7561 agcgcctcga cgctgaactc ctggctgccg ggcgtgccga acggcgcgtg ggtatagatc
     7621 tcgccctgca tgacgtagaa ctccttgtcg accggcggca ggccgccctc gggctcgacc
     7681 aggatcaggc catacatgcc gttggcgatg tggtgggcga ccatcggcgt cgcgcaatga
     7741 tagacgaaga tccccggcac cagcgccttg aaatccaggc gcttgctgtc gccgggatcg
     7801 acctgcaagt gcgccgcgcc gccgccgggg ccggtcgtcg catgaaagtc gaccgaatgg
     7861 atcatcgatg aatcggcgtc gttggcgagc accacctcga ccgtgtcgcc gacgcgcacc
     7921 ctgatgaacg gccccggcac ggtgccgttg aaggtccagt acggataggt cgtgccgtcg
     7981 gcgagctcgc cgtcgagctc gaccgcattg agctcgacgc gcacgcgctg gggcgggcgg
     8041 tcgccgatcg gcgccggcac cgccgccggg tccatgacga tgctcgcggc aacgggcgcc
     8101 gcttccgcga cggcggtcgt aacggtgaac ctcccctcca tgccggcctc gcggtggccg
     8161 ggcagcgagc agaagtaggg cgtggtgccg gcggcgcggg cgcggaaggc gacagtggtc
     8221 gccgcgccct tgtcgagcac gtgctgcgag gcgacgccgt gctcggcaat gacgatgtcg
     8281 tgctccgcgc cctcgccgtt gatcagcgtg atctggacca cgtcgccctc gccggccgac
     8341 agatcgggat tgacgatgcc gtcgatgtcg ccgccgaagc cgatgtagac cattcgtccc
     8401 tgggctatcc cggtttccaa agtgtagcgc acgtccggga tgtagccggc cggcagcgcc
     8461 gacgactccg cgccggccgc cgggagcggc gccgccgggg cggcggccgc ggccgcgggt
     8521 gcgccggcgc tcaggaaagc gatgacggca gcgcgatcct cagccttctt gaggccggga
     8581 aagaccatcc tggtgccggg caccagcttc atcggcccgg cgaggaacgc ctcgagcgtc
     8641 gccgcgtccc aggtcaggcc cgactgggcg agcgcgtccg agtagcggaa gccgccggcc
     8701 gcggcggcgg cgcgccccat caccccggcg aggctcggcc cgatcttgtg ctttccggcc
     8761 tcgaggctgt gacacgcggc gcacttgcgg aacacccgcg cgccgtcctc ggcgtcctcc
     8821 gtcgccgcgg cggagatcgc gccgaaggcg agtgcgagca gcagcccgat gcctgccagg
     8881 ctccgaaaac tgaacatcat ggccgaggcc tccctccaaa tgaggatgcg catgaaatcg
     8941 acgccggccc cgtctagcgc gccctcggcg cgccagccat gatctccatc aaggcagggc
     9001 gatcagaccg cgaagtagca ggcgcgcggg tggtggagga cgatcgccga ggtcgactgc
     9061 tcggggtgga gctgcgcctc ctcgcccagc accacgccga tcc
//
LOCUS       DASWHU010000056         6241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_23294, whole genome shotgun sequence.
ACCESSION   DASWHU010000056 DASWHU010000000
VERSION     DASWHU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23294"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VGB88_02065"
     CDS             <1..129
                     /locus_tag="VGB88_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9646253.1"
                     /translation="ARGVAEEAPGAYKDAGAVCEAAERAGLARRVARLKPLVCVKG"
     gene            complement(158..295)
                     /locus_tag="VGB88_02070"
     CDS             complement(158..295)
                     /locus_tag="VGB88_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646254.1"
                     /translation="MADFFNIEVSEDQAADENGHCEKRSDEAIQALAKAWIATALRAS
                     R"
     gene            complement(319..1761)
                     /locus_tag="VGB88_02075"
     CDS             complement(319..1761)
                     /locus_tag="VGB88_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9646255.1"
                     /translation="MTRIEPSRGLRPWLLIAAGAGLLLAGALAPAAAQEAPPSSPEPA
                     QPAGQPGAGRGEHDVYETRRDREYDPETTVFGPLPRLRLDDGNINLGAGALMQFDAAT
                     YDQNAQGGPDTAQALYTDLDTGLAGRRGIFLVNGIFYKDFIAFVSHDVFDPGTREIEG
                     LRSALLAWRGLDPLWLIVGQQNLPDPLDAATFSSQRTFMEESMSSGAFGYATGTPSLG
                     AAVLHRTKNHYVRVGLWGVPVDEYGHDSEGWGVHGRATYAPISERDRALHFGVSGYWR
                     KPTVRQGDVGGSERFASRPELHVDDLFFVDTGNIPRIDSFVFGGFEMLANRGPWNVQA
                     EYHRVWVDRYDGPHDAPFRDLTFDGYYVQTSYYLTGESQNFLSNFATPWRVQPNQEFD
                     LETGGWGAVEVAARFSHIDLDSGTGDLAHGGIRGGVADNYSLALNWYPTALTRVSVNY
                     VHSNIDNLSDSDLHEGGIIDAVGVRLQWEF"
     gene            2057..4351
                     /locus_tag="VGB88_02080"
     CDS             2057..4351
                     /locus_tag="VGB88_02080"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9646256.1"
                     /translation="MSTSKRHGGKRGTRTGVGLVSALLAGCSLLTPLPAPSTTAERLA
                     AFPTRGLPLERGVAIYWNDYQVPFIEAETDRDAAVALGLVHAHLRLGQMEMMRRISQG
                     RLAEMAGPLAADIDQGLHILDFGRAVADIERDLAPESRAWLEGFVAGVNHYLATVDKL
                     PHEFAVLGIAREPWTVRDVLTIGRLAGTDVNWLVWGGILSLRGRPDWPELWARLVANG
                     SASMPSFDAPGELSRAIEALAALSRSGSNSMAIASARTGTGSAIMASDPHVGIFVPDL
                     WLLVGLKSPSYHAVGLMGPALPVIAIGRNPWIAWGGTNMRAASSELYDLSGIERSEIS
                     ARTETIRVRWWFDRTITVRETRFGPVISDAPLFDDYDLPDLALRWTGFESSDEIGAML
                     AVMRARDFAAFRAAFESFAVPGQNMLYADSGGNIGQVMAVRLPARDGAPPPDLLADPA
                     EREPHWQEMRGVAELPFSYNPAAGFLVSGNNRPAETDIHVGYFFSPDDRVERMAELIR
                     AHNPVGVADVESFQQDVYMPSAVALRDVLLAKLEETGLAAVAEARAREAIRRLAEWDG
                     QYRVDSQGAVAFEQFRAAFTARFYALTFGGTDWLAFANVARIKALLIEDIERARAEDL
                     RPALAAGLARAAEGLDAFAGWGDMHRMELSHPLANVPLVGRRYRFDEFASGGSTDTLM
                     KTSHRATADRHAVGYGSNARHVSDLSDPDANFFVLLGGQDGWLNSANFLDQVPLWRDG
                     AYIRMPLTPKAVREGFARRIDLAP"
     gene            4362..5909
                     /locus_tag="VGB88_02085"
     CDS             4362..5909
                     /locus_tag="VGB88_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX9646257.1"
                     /translation="MLDVTPPFRLYAARRRRALEGLDAAAIQQATLRRLVKAAEQTRF
                     GRDHGFADIGSVAEFQARVPLRDYDRFWDEYWQAPFPRLVDCTWPGTMPYFAVSSGTT
                     RGVTKYIPCSHAMNRSNRRAAVDLLVHHVANRPHSRILGGRSFMLGGSTALIEQAPGI
                     YSGDLSGIAAKVMPRWARLRYFPPRELETIADWERKIECLARRSLREDIRALSGAPSW
                     LLLFLDRLAAIEGLDEARIAALYPRLELLVHGGVSFVPYRDRFALLLAGSGAETREVY
                     PASEGFFAIADRGDGEGLRLTLDIGLFYEFVPVDELESDAPSRRWIADVEPGVNYALV
                     VSTCAGLWAYVVGDTVRFVDLDPPRVLVTGRTAYTLSAFGEHLIGEELETAVAEAAGE
                     IGAEVVDFSAGSLFPRARGERGGHLYVVEFAAAIDDAARLAGFAAKLDAVLMRLNVDY
                     KDHRAEGFGLNPPRVHPVPPGTFAAWMRRRGKLGGQHKVPRVINDQALFEDLRRFAGD
                     LGAAEET"
     gene            5909..>6241
                     /locus_tag="VGB88_02090"
     CDS             5909..>6241
                     /locus_tag="VGB88_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9646258.1"
                     /translation="MRRDVILALAVLVLAAAAPAGATERELDFALFDAAGAGDAQRVA
                     ELLRDGASPAARDRFGNSALMFAVRSRDAATVRTLLAAGADPNLANLSRISALTTAAY
                     NGDAEIARL"
BASE COUNT          854 a         2213 c         2251 g          923 t
ORIGIN      
        1 gcgcgcggcg tcgccgagga ggcccccggc gcctacaagg acgcgggcgc ggtgtgcgaa
       61 gcggccgaac gcgccggcct cgcccgccgc gtcgcccgcc tcaagccgct ggtgtgcgtg
      121 aagggatgag gcgcaagccg cctcaccgtc gcccgcgtca tcgcgaggcg cgaagcgccg
      181 tggcgatcca ggccttggcg agtgcctgga ttgcttcgtc gctacgcttc tcgcaatgac
      241 cgttctcgtc ggccgcctga tcctccgaaa cttcgatgtt aaaaaaatcg gccatgcgcg
      301 ccgttcggcc tgcttcgctc aaaactccca ctgcaggcgg acgccgacgg cgtcgatgat
      361 gccgccctcg tgaaggtcgg agtcgctcag gttgtcgatg ttggagtgga cgtagttgac
      421 cgagacgcgg gtgagcgcgg tcgggtacca gttgagcgcg agcgaatagt tgtcggccac
      481 cccgccgcgg atgccgccgt gggcgaggtc gcccgtgcca ctatcgaggt cgatgtggct
      541 gaagcgcgcg gcgacctcga ccgcgcccca gccgccggtc tcgaggtcga actcctggtt
      601 cggctgcacc cgccacgggg tggcgaagtt ggacaggaag ttctgcgatt cgccggtcag
      661 gtaatagctg gtctggacgt agtagccgtc gaaggtgagg tcgcggaacg gcgcgtcgtg
      721 ggggccgtcg tagcggtcga cccatacccg gtggtactcg gcctgcacgt tccacggccc
      781 ccggttggcg agcatctcga acccgccgaa gacgaacgag tcgatgcgcg ggatattgcc
      841 ggtgtcgacg aagaacaagt cgtcgacgtg gagctcgggg cgcgaggcga agcgctcgct
      901 gccgccgacg tcgccttgcc tcaccgtcgg cttgcgccag tagcccgaga cgccgaaatg
      961 gagcgcgcgg tcgcgctcgg agatcggcgc ataggtggcg cgcccgtgca cgccccagcc
     1021 ctcgctgtcg tggccgtact cgtcgaccgg caccccccac aggccgacgc gcacgtagtg
     1081 gttcttggtc cggtgtagga ccgcggcgcc gagcgagggc gtgccggtgg cgtagccgaa
     1141 cgcgcccgac gacatcgatt cttccatgaa cgtgcgctgg ctcgagaagg tcgcggcatc
     1201 gagcgggtcg ggcaggttct gctggccgac gatcagccac agcgggtcga ggccgcgcca
     1261 ggcgagcagc gccgagcgca ggccctcgat ctcgcgcgtc cccggatcga agacgtcgtg
     1321 cgagacgaag gcgatgaagt ccttgtagaa gatgccgttg accaggaaga tgccgcgccg
     1381 tccggccaag ccggtgtcga ggtcggtgta gagcgcctgc gcggtatcgg gcccgccttg
     1441 cgcgttctgg tcgtaggtgg ccgcgtcgaa ctgcatcagc gcgccggcgc cgaggttgat
     1501 gttgccgtcg tcgaggcgca ggcgcggcag cggcccgaac accgtggtct cggggtcgta
     1561 ctcgcggtcg cggcgggtct cgtagacgtc gtgctcgccg cgcccggcac cgggctggcc
     1621 ggccggttgc gccggctcgg gcgaagaggg cggcgcctcc tgggccgcgg ccggcgccag
     1681 cgcgccggca agcaacaagc cggcgccggc ggcgatcagc agccagggcc gcaggccgcg
     1741 agagggttcg atacgggtca tcggtcggtt gcgaggcccc ccttcgacgc agtgtcatcc
     1801 ggcatcgcgc cgcgccccgt cgcgcgcccg atccagctgt ccccgtgcga tgacaatatc
     1861 ccgtgccgcg cgccggcacg ccccgaccgg ccttcagtcg gccgaagcag gtccccgggt
     1921 cgaaccccag ggatcggtcg gcgaaggccg gcggggtttg ccgccacgcg cccgcatcgg
     1981 taccttgatc gcgccgcacg ggtcaagccg cgcggcccat gtcgcggccc ggatcgccaa
     2041 ggacagcgag gcggaagtga gcacgagcaa gcgccacgga ggcaagcggg ggacgcgtac
     2101 cggcgtcggc ctggtgagcg ccctgctcgc cggctgcagc ctgctcaccc cgctgcccgc
     2161 gccatcgacc accgccgagc ggctggcggc cttcccgacc cgggggctgc cgctcgagcg
     2221 gggggtggcg atctactgga acgactacca ggtgccgttc atcgaggccg agaccgaccg
     2281 cgacgcggcg gtcgcgctcg gccttgtgca cgcgcatctg cgcctcggcc agatggagat
     2341 gatgcgccgc atctcgcagg ggcgcctcgc cgagatggcc ggccccttgg ccgccgacat
     2401 cgaccagggt ttgcacatcc tcgactttgg ccgcgcggtc gccgacatcg agcgcgacct
     2461 tgcgcccgag tcgcgcgcct ggctggaagg cttcgtcgcc ggcgtcaacc actaccttgc
     2521 gaccgtcgac aagctgccgc acgagttcgc cgtgctcggg atcgcgcgcg agccgtggac
     2581 ggtgcgcgac gtgctgacca tcggccggct cgccggcacc gacgtcaact ggctggtgtg
     2641 gggcggcata ctgagcctgc gtgggcgccc cgactggccc gagctgtggg cgcggctggt
     2701 cgccaacggc agcgcctcga tgccgagctt cgacgccccg ggcgagctca gccgggcgat
     2761 cgaggcgctc gccgccctgt cgcgctcggg cagcaacagt atggcgatcg cctccgcgcg
     2821 caccggcacc ggcagcgcga tcatggccag cgacccccat gtcggcatct tcgtgcccga
     2881 tttgtggctg ttggtcgggc tcaagtcgcc gtcgtaccac gcggtcgggc tgatgggccc
     2941 ggcgctgccg gtgatcgcga tcgggcgcaa cccctggatc gcgtggggcg gcaccaacat
     3001 gcgcgccgcc tcgagcgagc tctacgacct gagcggcatc gagcggagcg agatcagcgc
     3061 gcgcaccgag accatccgcg tgcgctggtg gttcgaccgc acgatcaccg tgcgcgagac
     3121 tcgcttcggc ccagtaatct cggacgcgcc gctgttcgac gactacgacc ttcccgacct
     3181 cgcgctgcgc tggaccggct tcgagtcgag cgacgagatc ggcgccatgc tggcggtgat
     3241 gcgggcgcgc gatttcgccg ccttccgcgc cgcgttcgag agcttcgccg tgcccggcca
     3301 gaacatgctc tatgccgact cgggcggcaa tatcggccag gtgatggctg tgcgcctgcc
     3361 ggcgcgcgac ggcgcgccgc cgcccgacct gctggccgac ccggccgagc gcgagccgca
     3421 ctggcaggag atgcggggcg tcgccgagct gccgttcagc tacaacccgg cggcggggtt
     3481 cctggtctcg ggcaacaacc ggccggccga gaccgacatc catgtcggct acttcttctc
     3541 gcccgacgac cgggtcgagc ggatggcgga gctgatccgc gcccacaacc cggtcggcgt
     3601 ggccgacgtc gagagcttcc agcaggacgt ctacatgccg tctgcggtgg ccttgcgcga
     3661 cgtgctgctg gccaagctcg aggagacggg gcttgcggcg gtcgccgagg cgcgcgcacg
     3721 cgaggcgatc cgccggctcg ccgagtggga cgggcagtat cgcgtcgact cgcagggcgc
     3781 ggtggcgttc gagcagttcc gcgccgcctt caccgcccgc ttctatgcgc tcaccttcgg
     3841 cggcaccgac tggctcgcct tcgccaacgt ggcgcggatc aaggcgctct tgatcgagga
     3901 catcgagcgg gcgcgggccg aggacctgcg cccggcgctc gccgccgggc tcgcgcgcgc
     3961 cgccgagggg ctcgatgcgt tcgcgggctg gggcgacatg caccgcatgg agctgtccca
     4021 tccgctcgcc aacgtgccgc tggtcggccg ccgttaccgg ttcgacgagt ttgccagcgg
     4081 cggctcgacc gacacgctga tgaagacctc gcaccgcgcc accgccgacc gccacgcggt
     4141 gggctacggc tcgaacgccc gccacgtctc cgacctgtcc gaccccgacg ccaacttctt
     4201 cgtcctgctc ggcgggcagg acggctggct caacagcgcc aacttcctgg accaggtgcc
     4261 gctgtggcgc gacggcgcct acatccggat gccgctcacg cccaaggcgg tgcgcgaggg
     4321 gttcgcgcgc cgcatcgacc tcgccccctg acgggggttg catgctcgac gtcacgccgc
     4381 cgttcaggct ctacgccgcg cgccgccggc gcgcactcga gggcctggac gcggcggcga
     4441 tccagcaagc aaccttgcgc cggctggtga aggcggcgga gcagacccgg ttcggccgcg
     4501 accacggctt cgccgacatc ggctcggtcg cggagttcca ggcgcgggtg ccgctgcgcg
     4561 actacgaccg cttctgggac gagtactggc aggcgccgtt cccgcgcctg gtcgactgca
     4621 cctggcccgg caccatgccc tacttcgcgg tctcgtccgg gaccacgcgc ggggtcacca
     4681 aatatatccc gtgcagccac gcgatgaacc gctcgaaccg gcgcgcggcg gtcgaccttc
     4741 tggtccacca cgtcgccaat cgtccgcaca gccgcatcct cggcgggcgc agcttcatgc
     4801 tcggcggcag cacggcgctg atcgagcagg cacccggtat ttacagcggc gacctgagcg
     4861 gcatcgcggc caaggtgatg ccgcgctggg cgcggctgcg ctacttcccg ccgcgcgagc
     4921 tcgagacaat cgccgactgg gagcggaaga tcgagtgcct ggcgcggcgc tcgctgagag
     4981 aggacatccg cgcgctcagc ggcgcgccga gctggctgct gctgttcctc gaccgcctgg
     5041 ccgcgatcga gggccttgat gaggcgcgca tcgccgcgct ctacccgcgc cttgagctcc
     5101 tggtccacgg cggggtgagc ttcgtgccct atcgcgaccg cttcgccctg ctgctcgccg
     5161 gcagcggcgc cgagacccgc gaggtctatc cggcgagcga gggcttcttc gccatcgccg
     5221 accgcggcga cggcgagggc ctcaggctca ccctcgacat cggcctgttc tacgagttcg
     5281 tgccggtgga cgagctcgaa agcgacgcgc cgtcgcggcg ctggatcgcc gacgtcgaac
     5341 ccggggtcaa ctacgcgctc gtggtctcga cctgcgccgg gctgtgggcc tatgtggtcg
     5401 gcgataccgt ccgcttcgtc gacctggatc cgccgcgggt gctggttacc gggcgcaccg
     5461 cctacacgct gtcggcgttc ggcgagcacc tgatcggcga ggagctggaa acggcggtcg
     5521 ccgaggcggc gggagagatc ggcgccgagg tcgtcgattt ctcggccggc agcctgttcc
     5581 cgcgcgcgcg gggcgagcgc ggcggccacc tgtacgtggt cgagttcgcc gcggcgatcg
     5641 acgacgcggc gcgcctggcg ggtttcgccg ccaagctcga tgcggttctc atgcgcctca
     5701 atgtcgatta caaggatcac cgggccgaag gcttcgggct gaacccgccg cgcgtccacc
     5761 ccgtgccgcc gggcacgttc gcggcgtgga tgaggcggcg cggcaagttg ggcggccagc
     5821 acaaggtgcc gcgggtgatc aacgaccaag cgctgttcga ggatctcagg cgcttcgccg
     5881 gcgacctcgg ggccgcggag gagacgtgat gcgacgtgac gtgatcctgg cgcttgccgt
     5941 gcttgtgctc gcggccgcgg cgccggcggg cgcgaccgag cgcgagctcg acttcgcctt
     6001 gttcgacgcc gccggcgcgg gcgacgcgca gcgggtggcg gagctgctgc gcgacggcgc
     6061 ctcgcccgcg gcgcgcgacc ggttcggcaa cagcgcgcta atgttcgcgg tgcgcagccg
     6121 cgatgccgcc acggtgcgga cattgctcgc tgccggcgcc gatcccaacc tggccaacct
     6181 gagccgcata tcggcgctca cgaccgccgc ctacaacggc gacgcggaga tcgcgcgcct
     6241 g
//
LOCUS       DASWHU010000057         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1134141, whole genome shotgun sequence.
ACCESSION   DASWHU010000057 DASWHU010000000
VERSION     DASWHU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134141"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VGB88_02095"
     CDS             complement(<1..411)
                     /locus_tag="VGB88_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9646259.1"
                     /translation="MLFEVSELDPKEQEVIGRIDEMRRRLGAQARHPRRWVAALRRTT
                     FARAIQGSNSIEGYAVSLEDAVAVASDEEPVEAAEETRRAVIGYRNAMTYILQLADDP
                     HFAYSEQLLRALHFMMLGYDLSKHPGRWRPGWVGV"
     gene            complement(511..897)
                     /locus_tag="VGB88_02100"
     CDS             complement(511..897)
                     /locus_tag="VGB88_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9646260.1"
                     /translation="MHITGLDHIVLTVADLERTCDFYRRVLGLEVVVFGEGRRALRFG
                     DQKINLHQAGAEITPNAPAAGPGTGDICFTTDTPIAEVVATLDSFLVEVELGPVAQTG
                     ARGPMTSVYFRDPDGNLVEVARYDQG"
     gene            1020..1772
                     /locus_tag="VGB88_02105"
     CDS             1020..1772
                     /locus_tag="VGB88_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9646261.1"
                     /translation="MFSLHGVRRGFDGVALFEHLDLELAAGRTTVLIGPSGCGKSTLI
                     RLLLGLLWPEAGEVRFAGTPLSPDTVLGVRRRMGYVVQGGGLFPHLSARGNATLMARH
                     IGWPRARIEARLAELAALTRFPADALDRYPLQLSGGQRQRVALMRALMLDPDVLLLDE
                     PFGALDPMVRHRLQTELREVFRGLAKTVVMVTHDLGEAGYFGDQVVLLRAGRIVQRGT
                     LSDLVERPAEAFVDDFVSAQRTLLDSLGRAAS"
     gene            1769..>3168
                     /locus_tag="VGB88_02110"
     CDS             1769..>3168
                     /locus_tag="VGB88_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9646262.1"
                     /translation="MRPAAILATLAVCAVARAAAAEVVIGSKAFTESVVLGELVARFA
                     EASGVAARHRRQLGGTRVLWQALKAGEIDLYAEYTGTIGHEILAGEGGEALADAAAMR
                     AALARHGVAMTGDLGFNNSYALGMRAETAARLGIAAISDLGAHPELRLGFSNEFMDRA
                     DGWPALSAAYRLAPASVRGLDHDLAYRGLEAGSIDVVDLYSTDAEIAYYGLAVLADDR
                     GHFPAYRAVILYREELARRAPALIERLALLAGAIDEAAMSALNARVKIGGETEARVAA
                     DFAAARFAIAGEVAADGWGTRLWRNSLDHLALVAISLSAAIVIAIPLGVLAARRARAG
                     HVILAAAGVVQTIPSLALFVFMIPLLGIGGPPAVMALFLYSLLPIIRNTHAGLAGIPA
                     DIIESAAAIGLAPAARLRLVELPLAGRAILAGIKTSAVINVGTATLGALIGAGGYGQP
                     ILTGIRLDDTGLILEGA"
BASE COUNT          412 a         1152 c         1112 g          492 t
ORIGIN      
        1 cactccgacc cagcccggcc gccagcggcc gggatgtttg ctcaggtcgt agccgagcat
       61 catgaagtga agcgcgcgca acagctgctc gctgtaggcg aagtgggggt cgtcggcgag
      121 ctgcaggatg taggtcatcg cgttccggta gccgatgacc gcgcgccgcg tctcctccgc
      181 cgcctcgacc ggctcctcgt cactggcaac cgccaccgcg tcctcgagac tgacggcgta
      241 accttcgatc gagttcgacc cctgaattgc gcgcgcgaac gtcgttcggc gcaacgctgc
      301 gacccagcgg cgtggatggc gcgcctgagc gcccagccgg cgccgcatct cgtcgatgcg
      361 cccgatgacc tcctgctctt tcggatcgag ttcggatacc tcgaatagca tcgcgctctt
      421 ttaaataaca ttcgacttaa gcgcaataat taattgcgtt tttcgcaagt taaaaataga
      481 tatttgcgcc gggcgggggc cggcaaggac ttacccctga tcgtagcggg cgacctcgac
      541 caggttgccg tcggggtcgc ggaagtagac cgaggtcatc ggcccccgcg ccccggtttg
      601 cgccaccggg ccgagctcga cctcgaccag gaagctgtcg agcgtcgcca ccacctccgc
      661 gatcggggtg tcggtggtga agcagatgtc gccggtgccg ggcccggcgg cgggcgcgtt
      721 cggcgtgatc tcggcgccgg cctgatgcag gttgatcttc tggtcgccga acctgagtgc
      781 ccggcggcct tccccgaaca ccaccacctc gaggccgagc acgcggcggt agaagtcgca
      841 ggtccgctcc aggtcggcga ccgtgagcac gatgtggtcg aggccggtga tgtgcatcgc
      901 cgccgctccg ctgtagcccg cgccacgccg gccatgatcg ccgcatcggc gcgcgcgagg
      961 caatcgccgc gtcaccaccc ggtgacggcg cgtatgagag ctgggctatg ctggcggcca
     1021 tgttctcctt gcacggcgtc aggcgcggct tcgacggcgt ggcgctgttc gagcacctcg
     1081 acctcgagct tgccgccggc cgcaccacgg tgctgatcgg gccctcgggc tgcggcaagt
     1141 cgaccctgat ccgcctgctg ctcgggctgc tgtggccgga ggcgggcgag gtgcgcttcg
     1201 ccggcacccc gctcagcccc gacaccgtgc tcggcgtgcg ccggcggatg ggctacgtgg
     1261 tccagggcgg cggcctgttc ccgcacctca gcgcgcgcgg caacgcgacg ctgatggcgc
     1321 gccacatcgg ctggccgcgg gcgcgcatcg aggcgcgcct cgccgagctc gccgcgctca
     1381 cacgctttcc cgccgacgcg ctcgaccgct atcccttgca gctctcgggc ggccagcgcc
     1441 agcgcgtcgc gctgatgcgc gcgctgatgc tggatcccga cgtgctgctg ctcgacgagc
     1501 cgttcggggc gctcgacccg atggtccgcc accgcttgca gaccgagctg cgcgaggtgt
     1561 tccgcggcct cgccaagacc gtggtcatgg tcacccacga cctcggcgag gccggctatt
     1621 tcggcgacca ggtggtgctg ctgcgcgccg ggcgcatcgt ccagcgcggc acgctcagcg
     1681 acctggtcga gcggccggcg gaagcgttcg tcgacgactt cgtctccgcc cagcgcacct
     1741 tgctcgacag cctcgggcgg gcggcgtcat gagaccggcg gctatcctgg cgacgcttgc
     1801 ggtctgtgcc gtggcgcgcg ccgcggcggc ggaggtggtg atcggctcga aggcgttcac
     1861 cgagtcggtg gtgctcggcg agctggtcgc gcgcttcgcc gaggcgagcg gcgtggcggc
     1921 gcgccaccgc cgccagctcg gcggcacgcg ggtgctgtgg caggcgctga aggcgggcga
     1981 gatcgacctc tatgccgaat ataccggcac catcggccac gagatcctgg ccggcgaggg
     2041 cggcgaggcg ctcgccgacg cggccgcgat gcgtgccgcg ctggcccgcc acggcgtcgc
     2101 catgaccggc gacctcggct tcaacaacag ctacgcgctg ggcatgcgcg ccgagaccgc
     2161 cgcacgcctc ggcatcgccg ccatctcgga cctcggcgcg catcccgagc tcaggctcgg
     2221 cttcagcaac gagttcatgg accgcgccga cggctggccg gcgctcagcg cggcctatcg
     2281 gctcgcgccc gcgagcgtga ggggcctcga ccacgacctc gcctatcgcg gcctcgaggc
     2341 gggcagcatc gacgtcgtcg acctgtactc gaccgacgcc gagatcgcct attacgggct
     2401 cgcggtgctg gccgacgacc gcggccactt cccggcctac cgggcggtga tcctgtaccg
     2461 ggaggagctc gcccgccgcg cgccggcatt gatcgagcgc ctcgcgctgc tcgcgggcgc
     2521 gatcgacgag gccgcgatga gcgcgctcaa tgcccgggtc aagatcggcg gcgagaccga
     2581 ggcgcgggtc gccgccgact tcgccgccgc ccgcttcgcc atcgcgggcg aggtcgccgc
     2641 ggacggctgg ggcacgcggc tgtggcggaa cagcctcgac cacctggcgc tggtcgccat
     2701 ctcgctgtcg gccgcgatcg tgatcgcgat ccccttgggc gtgctcgccg cgcggcgtgc
     2761 gcgcgccggc cacgtcatcc tcgccgccgc cggcgtcgtc cagaccatcc cctcgctcgc
     2821 cctgttcgtg ttcatgatcc cgctgttggg aatcggcggg ccgccggcgg tgatggcgtt
     2881 gttcctgtac agcctgctgc cgatcatccg caacacccat gccgggctcg ccggcatccc
     2941 ggcggacatc atcgagtcgg cggcggcgat cgggctcgcg ccggcggcgc ggctgcgcct
     3001 ggtagagctg ccgctcgcgg ggcgggcgat cctggccgga atcaagacct cggcggtgat
     3061 caatgtcggc accgccaccc tcggcgcgct gatcggcgcc ggcggctacg gccagccgat
     3121 cctgaccggc atccgcctcg acgacaccgg cctgatcctc gagggcgc
//
LOCUS       DASWHU010000058         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1134167, whole genome shotgun sequence.
ACCESSION   DASWHU010000058 DASWHU010000000
VERSION     DASWHU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134167"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2739)
                     /locus_tag="VGB88_02115"
     CDS             complement(<1..2739)
                     /locus_tag="VGB88_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9646263.1"
                     /translation="MTLHVALNHQTDYRYDRLVRMGPQVVRLRPAPHARTPIVSYALN
                     IEPTGHFLNWQQDPHGNYLARIVFPDKVRAFHVEVDLVADMVVHNPFDFFLEPEAEQY
                     PFRYEPALMADLAPYLKTEAAGPLLTAWLEPYRDREPLRTVDVLVELNARLQSDVEYV
                     IRMEPGVQALEETLSLGRGSCRDTGWLLVQVLRHLGIASRFISGYLIQLVADQKALDG
                     PSGTDRDFTDLHAWAEAYLPGAGWVGLDPTSGLFTGEGHIPLAATPHPATAAPISGAL
                     DECEIDFSHRMSVTRVAESPRVTKPYSDDQWREIDAFGRRLDAELQVNDVRLTIGGEP
                     TFVAVDDPDAPEWNTAAVGPTKRRLAAALIKRLRDRFAPGGLLHFGQGKWYPGEPLPR
                     WAFALYWRRDGRPLWHDDALIADDAHDYRVSIDHARSFAEGIARRVEVEVASVMPAYE
                     DPWHFIEQERKLPENLDPARNKLDDPLARARLAKVFERGLGQPSGFVLPLQRWNARDG
                     SRRWRSQPWSTRARHLFLVPGDSPLGLRLPTDSLPYVTPSNYPYIVPADPFAERAELP
                     AIDPRRQPFLRAHGGPGPGFEPPQRVVGQVAEVGGRRVVRAALSVEPRDGRLCVFLPP
                     TETVEDYLDLVAAVEDAAAEHATPVHLEGYTPPPDPRLEVIKVTPDPGVLEVNIHPAR
                     DWPEMAAITAGLYEDARASRLATEKFMLDGRHTGTGGGNHVVVGGPTPGDSPFLRRPD
                     LLRSLIAYWQNHPALSYLFSGLFIGPTSQAPRIDEARHDSLYELELAFTQVPDPDAGA
                     SVPPWLVDRIFRNLLIDVSGNTHRAEICIDKLYSPDGPAGRLGLVEFRAFEMPPHARM
                     SLAQQLLLRALIARFWKSPYRAGLVRWGTGLHDRFMLPHFVWQDLCEVIADMTRAGY"
     gene            complement(2829..3314)
                     /locus_tag="VGB88_02120"
     CDS             complement(2829..3314)
                     /locus_tag="VGB88_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9646264.1"
                     /translation="MTRRGRRAWTLAAGATVAAAGLAWWLASPPAADARIALRPDDRE
                     TVARGGEIYGRECASCHGGTLEGQSGWRGRRPDGRLPAPPHDETGHTWHHPDAQLFAL
                     TKVGPAALIGGGYASDMPAFEGVLSDPDIIAVLSFIKSTWPRPIRDRHDEINRRARAG
                     E"
     gene            complement(3311..3715)
                     /gene="cueR"
                     /locus_tag="VGB88_02125"
     CDS             complement(3311..3715)
                     /gene="cueR"
                     /locus_tag="VGB88_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEX9646265.1"
                     /translation="MNIGEAEKQSGLPAKTIRYYEEVGLVRPARGANRYRSYSDHDLH
                     KLRFLQRARGLGFSVGDCRTLLSLYEDRRRSSADVKAIGERHLARIDAKIAELASLRA
                     ALAGLVEACRGDHRPDCPILDDLAGATGDGEP"
     gene            complement(3728..>4576)
                     /locus_tag="VGB88_02130"
     CDS             complement(3728..>4576)
                     /locus_tag="VGB88_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9646266.1"
                     /translation="AAGLERASEHPLAEAIVAGAEDRGLSLAAPTDFRSVTGKGVVGR
                     IGGAEVALGNSALMAELGVDGSALAAKAEELRADGATVMLVAVAGRAAGLIAVADPIK
                     ETTPTALSALRAEGLRVVMLTGDNRTTAEAVARRLGIDEVKAEVLPDRKNQVVRELRA
                     AGRIVAMAGDGVNDAPALAQADVGVAMGSGTDVAIESAGVTLVKGDLNGIVRARRLSR
                     ATMRNIRQNLFFAFIYNALGVPIAAGVLYPFFGLLLSPVIAAAAMSLSSVSVVGNALR
                     LRRVRI"
BASE COUNT          605 a         1602 c         1733 g          636 t
ORIGIN      
        1 gtagccggcg cgggtcatgt cggcgatcac ctcgcagagg tcctgccaga cgaagtgggg
       61 cagcatgaac cggtcgtgca gcccggtgcc ccagcgcacc aggccggcgc ggtaggggct
      121 tttccagaag cgcgcgatca gcgcgcgcag caacagctgc tgggcgaggc tcatgcgcgc
      181 gtgcggcggc atctcgaagg cgcggaactc gactaggccg agccggccgg cgggcccgtc
      241 gggcgagtac agcttgtcga tgcagatctc ggcccggtgg gtgttgcccg agacgtcgat
      301 cagcaggttg cggaagatgc ggtcgaccag ccacggcggc acgcttgcgc cggcatcggg
      361 gtcgggcacc tgcgtgaatg cgagctcgag ctcgtagagc gagtcgtggc gcgcctcgtc
      421 gatgcgcggc gcctggctgg tcgggccgat gaacaggccc gagaacaggt aggacagcgc
      481 cggatggttc tgccagtagg cgatcaggct gcgcagcagg tcggggcggc gcaggaacgg
      541 gctgtcgccg ggggtcggcc cgccgaccac cacgtggttg ccgccgccgg tgccggtgtg
      601 gcggccgtcg agcatgaact tctcggtcgc gagccggctc gcgcgcgcgt cctcgtagag
      661 gccggcggtg atcgccgcca tctccggcca gtcgcgggcg gggtggatgt tgacctcgag
      721 caccccgggg tcgggcgtca ccttgatcac ctcgagcctg ggatcgggcg gcggcgtgta
      781 gccttccagg tgcaccgggg tcgcgtgctc ggcggcggcg tcctcgaccg cggcgaccag
      841 gtcgagatag tcctcgaccg tctcggtcgg cggcaggaac acgcacagcc gcccgtcgcg
      901 cggctcgacc gatagcgcgg cgcggaccac gcgccggccg cccacctcgg ccacctgccc
      961 gaccacgcgc tgcggcggct cgaagcccgg gccgggcccg ccgtgggcgc gcaggaacgg
     1021 ctggcggcga gggtcgatgg cgggcagctc ggcgcgctcc gcaaacgggt cggccgggac
     1081 gatgtaggga tagttgctcg gcgtcacata tggcagcgag tcggtcggca ggcgcaagcc
     1141 gagcggcgaa tcgccgggca ccaggaacag gtggcgcgcg cgcgtcgacc acggctggct
     1201 gcgccagcgg cgcgagccgt cgcgcgcgtt ccagcgctgc aacggcagga cgaaaccgga
     1261 cggctgcccc aggccgcgct cgaacacctt ggccaggcgc gcgcgcgcca gcgggtcgtc
     1321 gagcttgttg cgcgccgggt ccaggttctc cggcagcttg cgttcctgct cgatgaaatg
     1381 ccaggggtcc tcgtaggcgg gcatcaccga ggcgacctcg acctcgaccc ggcgcgcgat
     1441 gccttcggcg aagctgcggg cgtggtcgat cgagacccgg tagtcgtgcg cgtcgtccgc
     1501 gatcagggcg tcgtcgtgcc acagcggccg gccgtcgcgc cgccaataga gcgcgaacgc
     1561 ccagcgcggc aacggttcgc ccggatacca tttgccttgc ccgaaatgca gcagcccgcc
     1621 cggcgcgaag cggtcgcgca ggcgcttgat cagggcggcg gcgagcctgc gcttggtcgg
     1681 cccgaccgcg gcggtgttcc attcgggcgc atcggggtcg tcgaccgcga cgaaggtcgg
     1741 ctcgccgccg atggtgagcc ggacgtcgtt cacctgcagc tcggcgtcga gccggcggcc
     1801 gaaggcgtcg atctcccgcc actggtcgtc gctatagggc ttggtcacgc gcggcgattc
     1861 ggcgacgcgg gtgaccgaca tccggtggct gaagtcgatc tcgcactcgt ccagcgcgcc
     1921 gctgatcggc gccgcggtcg cggggtgcgg ggtcgcggcg agcggtatat ggccctcgcc
     1981 cgtgaacagg cccgaggtcg ggtcgaggcc aacccagccc gcgcccggca ggtaggcctc
     2041 cgcccaggca tgcaggtcgg tgaagtcgcg gtcggtgccc gacggcccgt cgagcgcctt
     2101 ctggtcggcg accagctgga tcaggtagcc ggagatgaac cggctggcga tgccgagatg
     2161 cctcaagacc tggaccagga gccagcccgt gtcgcggcac gagccgcggc cgagcgacag
     2221 cgtctcttcc agtgcctgta cgcccggctc catgcggatc acgtactcga cgtcggactg
     2281 caggcgggcg ttgagctcga ccaggacgtc gaccgtgcgc aggggctcgc ggtcgcgata
     2341 cggctccagc cacgccgtca gcagcggccc ggcggcctcg gtcttcagat acggcgcgag
     2401 gtccgccatc agcgccggct cgtagcggaa cgggtactgc tcggcctcgg gctcgaggaa
     2461 gaagtcgaac gggttgtgga ccaccatgtc ggcgaccagg tcgacctcga cgtggaacgc
     2521 gcgcaccttg tcggggaaca cgatgcgcgc gaggtagttg ccgtgcgggt cctgctgcca
     2581 gttgaggaaa tgcccggtgg gctcgatgtt gagcgcgtag ctcacgatcg gggtgcgggc
     2641 gtgcggcgcc ggccgcaggc gtaccacctg cgggcccatg cgcacgagcc ggtcgtagcg
     2701 atagtcggtc tggtggttga gggcgacgtg caatgtcatc ggtcgggccg gtcctggggg
     2761 cgccgggatg gtagcgcaaa tcggccatcg accaagaccc cggtggcgcc ggcgcggccc
     2821 ctggtatcct attcgcccgc ccgagcgcgc cggttgatct cgtcgtggcg gtcgcggatc
     2881 ggccgcggcc aggtgctctt gatgaacgac agcacggcga tgatgtccgg gtcgctgagc
     2941 acgccctcga acgccggcat gtcgctcgca tagccgccgc cgatcagcgc cgccggcccg
     3001 accttggtga gggcgaacag ctgcgcgtcg ggatgatgcc aggtgtggcc ggtctcgtcg
     3061 tgaggcggcg ccggcagccg gccgtcgggg cgcctgccgc gccagccgga ctggccctcg
     3121 agggtgccgc cgtggcagct cgcgcattcg cggccataaa tctcgccgcc gcgcgccacc
     3181 gtctcgcggt cgtcgggcct gagcgcgatc cgcgcatcgg cggccggcgg gctcgcgagc
     3241 caccaggcaa ggcccgccgc cgccacggtc gcgccggcgg ccagcgtcca cgcgcggcgc
     3301 ccccggcggg tcacggctcg ccgtcgccgg ttgcgcccgc gaggtcgtcg aggatcgggc
     3361 agtcggggcg gtggtcgccg cggcacgcct cgaccaggcc ggcgagcgcc gcgcgcaggc
     3421 tcgccagctc ggcgattttg gcgtcgatgc gcgccaggtg gcgctcgccg atcgccttga
     3481 cgtcggccga gctgcgccgg cggtcctcat agagcgacag cagcgtgcgg cagtcgccga
     3541 ccgagaagcc gaggccgcgc gcgcgctgca ggaagcgcag cttgtgcagg tcgtggtcgc
     3601 tgtagctgcg gtagcggttg gcgccgcgcg cgggcctgac caggccgacc tcctcgtagt
     3661 agcggatggt cttggccggc agccccgact gcttctccgc ctcgccgatg ttcatcgcaa
     3721 cccctcctca gatgcggacc cgcctcagcc tgagcgcatt gcccaccacc gacaccgagc
     3781 tcaggctcat cgccgcggcg gcgatcaccg ggctcagcag caggccgaag aacgggtaca
     3841 gcacgccggc cgcgatcggc acgccgagcg cattgtagat gaaggcgaag aacaggttct
     3901 ggcggatgtt gcgcatggtc gcgcggctca gccggcgcgc acgcacgatg ccgttgaggt
     3961 cgcccttgac cagggtgact ccggcgctct cgatggcgac gtcggtgccg ctgcccatgg
     4021 cgacgccgac gtcggcctgg gcgagcgccg gggcgtcgtt gaccccgtcg ccggccatgg
     4081 cgacgatgcg cccggccgcg cgcagctcgc gcaccacctg gttcttgcgg tcgggcagca
     4141 cctcggcctt gacctcgtcg atgccgagcc ggcgggcgac cgcctcggcg gtggtgcggt
     4201 tgtcgccggt cagcatcacc acccgcaggc cttccgcacg cagcgcgctg agcgccgtgg
     4261 gcgtggtctc cttgatcggg tcggcgaccg cgatcaggcc ggcggcgcgc ccggccaccg
     4321 ccaccagcat gaccgtggcg ccgtcggcgc gcagctcctc ggccttggcc gcgagcgccg
     4381 agccgtcgac gccgagctcc gccatcagcg cgctgttgcc gagcgcgacc tcggccccgc
     4441 cgatgcgccc gaccacgccc ttgccggtca ccgagcggaa gtcggtcggc gcggcgaggc
     4501 tgaggccgcg gtcctcggcg ccggcgacga tcgcctcggc gagcgggtgc tcgctcgccc
     4561 gctcgaggcc ggcggc
//
LOCUS       DASWHU010000059         9272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2241355, whole genome shotgun sequence.
ACCESSION   DASWHU010000059 DASWHU010000000
VERSION     DASWHU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241355"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..939
                     /locus_tag="VGB88_02135"
     CDS             172..939
                     /locus_tag="VGB88_02135"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX9646267.1"
                     /translation="MAVAAVHDPHAEELRRAREDLAAAYRLCQRFGLNEGIDNHLTLA
                     VPGGDDRFLLIPYGLHWSEVTASALLIVDADGNTVEGHGFVEPTAFFIHAAIHKARRD
                     ARCVMHTHMPYALALAMIEGGRLAMAEQNALRFFGRIAYDDSYGGGAYGWDEGGRIAA
                     ALGKADALLMANHGVTVAGPTVAAAWDDLYYLEQACRAQVLAMSTGRPLRRVPDAIAA
                     EVAASVRAEATGRSANIERHFEALKRVLIRDGADFRA"
     gene            complement(1065..1856)
                     /locus_tag="VGB88_02140"
     CDS             complement(1065..1856)
                     /locus_tag="VGB88_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9646268.1"
                     /translation="MDDRPRFSVMEMFRLDGRVAVVTGAGKGLGRIAALALAEAGARL
                     AVLDLDAGTAAQTVREIAECGGTARAFTCDVGEEAQVTATFDAVAEAFGGIDILVNNA
                     GIARREPAEDMPTATWDEVIRINQTAVFLCTREAAKRMLAKGRGAIVNVASIMGVTGG
                     GYYPNLPYHASKGAVVNMTRALAAEWGRRGVRVNAIGPTFTETDLTASLRANKQVMAY
                     VEERTPMGRFAKPEEMAGAILYLASPAASAVTGITLLVDCGWTAI"
     gene            complement(1869..2516)
                     /locus_tag="VGB88_02145"
     CDS             complement(1869..2516)
                     /locus_tag="VGB88_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX9646269.1"
                     /translation="MADLAARLERLYTGVVYDTMSDLGLAGQALPAAIKPLDQAMRLA
                     GPVWTLSGVRTPSVGRHESLLAWTGFLSAAPAGHVVVCQPNDSEIALMGELSAETLKS
                     RGVLGYVADGGCRDVDFIVRLGFPVYCRFVTPADIAGRWRVDEMGTAVRIGGLDVHTG
                     DWLVADRDGVVIVPADRAEEVVAKAEQAAGAENKIRAAILSGTDPRDAYLKYGKF"
     gene            complement(2599..2826)
                     /locus_tag="VGB88_02150"
     CDS             complement(2599..2826)
                     /locus_tag="VGB88_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646270.1"
                     /translation="MGSPRSRAAAVALALACAAVLSACQDQRNEFNPVRILCPGNFDA
                     RTNRCVIPTGRNPARDYFNGTDAAPGAPPPP"
     gene            complement(2886..3320)
                     /locus_tag="VGB88_02155"
     CDS             complement(2886..3320)
                     /locus_tag="VGB88_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX9646271.1"
                     /translation="MSAPAAAETGAVTRGAVRLLESLGYRTLTEMRLASGRRVDAIGL
                     DGRGRFAVVEVKTSLTDLRADDKWRDYLPYCDLFYFAVPPGFRLEAVPDEVGLIIADR
                     FAGEVARASPLVPMAALRRRSQTLLFAHVASARLYRFVESGV"
     gene            complement(3463..3591)
                     /locus_tag="VGB88_02160"
     CDS             complement(3463..3591)
                     /locus_tag="VGB88_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX9646272.1"
                     /translation="MTYDPNQYLQSHRNTWRGFTRLVIYSAASVALTLIVMALFLL"
     gene            complement(3670..4314)
                     /gene="maiA"
                     /locus_tag="VGB88_02165"
     CDS             complement(3670..4314)
                     /gene="maiA"
                     /locus_tag="VGB88_02165"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX9646273.1"
                     /translation="MKLYGYYRSSAAYRVRIVLNLKGLAYEQVFVHLRRGGGEQFRPD
                     YVKLNPQALVPTLDDDGVVLTQSLAIINYLEETRPEPRLVPADPAARARVRALALAVA
                     CDIHPLNNLRVLQYLKDELDIDEDARNRWYRHWVELGLGAMEQMVAGHPATGRFCHGD
                     FPGIADACLVPQLANARRFGCDVGVCPTLVTIEGACAELPAFRAAAPENQPDAE"
     gene            complement(4322..5320)
                     /locus_tag="VGB88_02170"
     CDS             complement(4322..5320)
                     /locus_tag="VGB88_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9646274.1"
                     /translation="MKLGSLKEGGRDGTLVVVARDLGIAVRVPHIAPTLQSAIEAWQA
                     VAPELEAVARGLDAGRRDDAFALDPAALASPLPRAYQWVDASAYLNHVELVRRARGAE
                     MPASFLDDPLMYQGGSDAFIGPCDPILAASEDWGIDLEGEVAAILDDVPMGAMPAEAG
                     RHVKLLMLVNDVSLRGLIPVELAKGFGFFQGKPATAFSPVAVTPDELGAAWDGAKVNL
                     PLIVRLNGKQVGRANAGVDMQFDFPRLIAHAARTRVLTAGTILGSGTISNRDRAAGWS
                     CIAEVRMIETIERGAPITPFMKFGDRVRIEMLDHAGASIFGAIDQVVERYRGPGED"
     gene            complement(5320..6471)
                     /locus_tag="VGB88_02175"
     CDS             complement(5320..6471)
                     /locus_tag="VGB88_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX9646275.1"
                     /translation="MKHWISHPWLEGTARRQAHCDLPEGSYERELGREGFFGPSTQMI
                     HRHPPTGWSDFSGPLRPRLFDLNRLAGTAEMPWQAQEVLANASLRIRFWRAEGNMPAL
                     ARNADGDDILFVHDGRGRLFCDYGRIDLSDGDYLVLPRGTMWRTEFDGAAELLLIEAT
                     NDAFRLPEKGLVGRHAIFDPAVLEAPHIDDAFRAQQDERPWRVEIKKGNAVSTATFPY
                     NPLDALSWHGDLAPVRLNWRDIRPIMSHRVHLPPSAHTTFLAGRFVVCTFVPRPFETD
                     PGALKVPFFHSNDDYDEVIFYHRGNFFSRDNIDRGMITFHPSGFTHGPHPKALEHALV
                     QPKPATDEVAVMIDARDSLEVAALPAGVENPDYVRSWQGARVPAHAAAE"
     gene            6571..7764
                     /locus_tag="VGB88_02180"
     CDS             6571..7764
                     /locus_tag="VGB88_02180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX9646276.1"
                     /translation="MAARALIQLSAARARRRALIRTTRGCAGGGGVAEPSDESRKLIA
                     LIRSLRQALERIETPACDAAASALATVATEIAAAGPGADLLVVADQLPAARAGRAQDP
                     ADGVLDVRNRQFRDILSACPLGVAVISRRGWKRLFINHYLVDMFKAPSMEAFQRQELI
                     DSWVDKAEFARAARNIAADVDHVDFEAMRRCFDGSMIWVLMNSLPIVFEGEPARVFWH
                     IDITERKRMEDELRLLATTDVLTGVANRGHFLATVDREVERQRRYGPPCGLLMLDIDH
                     FKSVNDSFGHACGDQVLRHVARLCLENIRGIDTLGRMGGEGFAVVVPETDDAGVLRLA
                     ERLRLAVAGSVLDSETGASVRVTLSVGATLHRLHDDAVEVTFARADKALYEAKHAGRN
                     RVIFY"
     gene            7945..9267
                     /locus_tag="VGB88_02185"
     CDS             7945..9267
                     /locus_tag="VGB88_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9646277.1"
                     /translation="MDRSRAATILSLALPIIGGMASQNLFNLVDTAMVGRLGPAALAG
                     VGIASFANFTCVAVVVGLSAGVQAMSARRKGEGRIAETAVPLNGGLIIALGLSLPLSA
                     ALIWAVPRLFPLLNADPAVIAEGVPYFEARLVAMAAVGINFAFRGFWNGIGQSGVYLR
                     TLLVMHACNIVLDYVLIFGAFGAPRLGTLGAGIGTAVATYVGAGTYLWLAWRRARANG
                     FLHRVPRRATMLTMLRLAIPSSTQQLFFSAGFTVLFWIVGLVGTAEVAAANVLINVML
                     VAILPGLGFGLAAASLVGQALGAGDAADARRWGWDVVKVAAATVAVLGLPMLAFPDAI
                     LGVFLREPETLALARLPLRLVGATIAIEAVAMVLMNALLGAGAARQVMVVSITLQWLA
                     FLPLAWLVGPGLGYGLLGIWALQVVYRAVQALVLTGLWRRGRWAEIVV"
BASE COUNT         1251 a         3252 c         3388 g         1381 t
ORIGIN      
        1 gacgaaggct agaacatggt ccgctcaggt tggatcaacc cccctcaccc ttccctctcc
       61 acccgcgagg tggggggaga gggttgcgaa gggttggcgc ggcaccgccg cgccttaccc
      121 ggagccgggt gaggggggcc acgccggcgc ggccggctga gggttcgggc aatggcggtc
      181 gccgcggttc acgaccccca tgccgaggag ctgcggcggg cgcgcgagga cctggccgcg
      241 gcctaccggc tgtgccagcg cttcgggctc aacgagggca tcgacaatca cctgaccctc
      301 gccgtgccgg gcggcgacga ccgcttcctg ctgattccct acgggctgca ctggtcggag
      361 gtgacggcga gcgcgctcct gatcgtggac gcggacggca acacggtcga aggccacggc
      421 ttcgtcgagc cgaccgcgtt cttcatccat gccgcgatcc acaaggcgcg ccgggacgcg
      481 cgctgcgtga tgcacaccca catgccgtac gcgctcgccc tcgccatgat cgagggcggc
      541 cggctcgcga tggcggagca gaacgcgctc cgcttcttcg gccgcatcgc ctacgacgac
      601 agttacggcg gcggcgccta tggctgggac gagggcgggc gcatcgccgc cgcgctcggg
      661 aaggccgacg cgctgctgat ggccaaccac ggcgtcaccg tcgccgggcc gacggtcgcg
      721 gcggcgtggg acgacctcta ctacctcgag caggcgtgcc gcgcccaggt gctcgccatg
      781 tcgacgggaa ggccgctcag gcgcgtcccc gacgcgatcg ccgccgaggt cgccgccagc
      841 gtgcgtgccg aggccaccgg gcgctccgcc aacatcgagc gccacttcga ggcgctgaaa
      901 cgcgtgctga tccgcgacgg cgccgacttc cgcgcgtgag ccgcgctttc gacaccgtcg
      961 tcatgccggc cttgagccgg catccatgcg accgggccgg cggcacgatg gaccccgaat
     1021 ccgcctgcgg cgctccgggg tgacggaaga ggaagagcca gtcctcagat cgcggtccag
     1081 ccgcagtcga cgaggagggt gatgccggtc accgcggagg cggcggggga ggcgaggtag
     1141 aggatcgcgc cggccatctc ttcgggcttc gcgaaccggc ccatcggcgt gcgctcctcg
     1201 acataggcca tcacctgctt gttcgcgcgc aacgatgcgg tgaggtcggt ctcggtgaag
     1261 gtcggcccga tcgcgttcac gcgcaccccg cgccggcccc actcggcggc gagcgcgcgg
     1321 gtcatgttga ccaccgcgcc cttcgatgcg tggtagggca ggttcgggta gtagccgccg
     1381 ccggtgaccc ccatgatcga ggcgacgttg acgatcgcgc cgcggccctt ggcgagcatg
     1441 cgcttcgccg cctcgcgcgt gcacaggaac accgccgtct ggttgatgcg gatgacctcg
     1501 tcccaggtcg cggtgggcat gtcctcggcc ggctcgcggc gggcgatgcc ggcgttgttg
     1561 accaggatgt cgatgccgcc gaaggcctcg gcgacggcgt cgaaggtggc ggtcacttgc
     1621 gcttcctcgc ccacgtcgca ggtgaacgcg cgcgccgtgc ccccgcactc ggcgatctcg
     1681 cgcacggtct gggcggcggt gccggcgtcg aggtcgagca cggcgaggcg cgcgccggcc
     1741 tcggcgagcg cgagggccgc gatccgcccg agccccttgc cggcgccggt gacgaccgcg
     1801 accctcccgt cgaggcggaa catctccatc accgagaagc gcggccggtc gtccatcttc
     1861 gggctccttc agaacttgcc gtatttgagg taggcgtctc tggggtcggt gcccgacagg
     1921 atcgccgcgc gaatcttgtt ctcggcgccc gccgcctgct cggccttggc caccacctcc
     1981 tcggcgcggt cggcgggcac gatcaccacc ccgtcgcggt cggcgaccag ccagtcgccg
     2041 gtatgcacgt cgaggccgcc gatgcgcacg gccgtgccca tctcgtcgac ccgccagcgg
     2101 ccggcgatgt cggccggggt gacgaaccgg cagtagaccg ggaagccgag cctgacgatg
     2161 aaatcgacgt cgcggcagcc gccgtcggcg acgtagccca gcaccccgcg tgacttgagc
     2221 gtctccgccg acagctcgcc catcagcgcg atctccgagt cgttcggctg gcacaccacc
     2281 acgtggcccg ccggcgccgc cgacaggaac ccggtccagg cgagcagcga ctcgtggcgg
     2341 ccgaccgagg gcgtgcgcac gccgctcaac gtccacaccg gccccgccag ccgcatcgcc
     2401 tgatccagcg gcttgatcgc cgccggcagc gcctggccgg caagcccgag gtcgctcatg
     2461 gtgtcgtaga ccacgcccgt gtacaggcgc tcaagccgtg ccgcgaggtc ggccatcggg
     2521 tccctcaagt gctgcgcgca ggatgcgccg atggcgaggt tgtagcggct cggggcgcag
     2581 ccgtcacgcc cgctgcggtc agggcggcgg gggcgcgccg ggcgccgcgt cggtgccgtt
     2641 aaagtagtcg cgcgccgggt tcctgccggt cgggatgacg cagcggttgg tgcgtgcatc
     2701 gaagttgccc gggcacagga tgcggaccgg attgaactcg ttgcgctggt cctggcacgc
     2761 cgacagcacg gccgcgcatg ccagcgccag cgcgaccgcc gccgcgcggc ttctcgggct
     2821 tcccatggct cggggctgct cctggctcgg gggtgagtgt ggggacgaag gcgccacgcg
     2881 cgccactata cccccgattc gacgaaccgg tacaggcggg cactcgcgac gtgcgcgaac
     2941 agcagcgtct ggctgcgccg gcgcagggcc gccatcggca cgagcggact tgcccgcgcc
     3001 acctcgccgg caaagcggtc ggcgatgatc aggccgacct cgtcggggac cgcctcgagg
     3061 cggaacccgg gcggcacggc gaagtagaac aggtcgcagt agggcagata gtcgcgccac
     3121 ttgtcgtccg cgcgcaggtc ggtgaggctg gtcttgacct cgaccacggc gaagcggccg
     3181 cggccgtcga ggccgatcgc gtcgacccgc cggcccgaag ccaggcgcat ctcggtgagc
     3241 gtgcgatagc cgaggctctc gagcaggcgc accgcgccgc gggtcaccgc gcccgtctcg
     3301 gccgcggcgg gcgcgctcac caggtcgggg cggcttcccg gaatgcgcgc ccgcgccggc
     3361 cgcgccggcg tcgtcggcgc cggcggtccg gggaaaaagg gaagcgcgtg ctgcatggcg
     3421 ccgcccggcc gcgctcgctc gacgtcctgc ggcctcggcg cctcagagca ggaacaacgc
     3481 catcacgatc agggtcagcg ccaccgaggc agcgctgtag atgaccagcc gggtgaaccc
     3541 gcgccacgtg ttgcggtggg actggaggta ctggttcgga tcgtaggtca tggccgccgg
     3601 gtccccgcct tggtcgacac cggcggtact gatagccaag cccgcgccca ggcacaagcc
     3661 cggcggcgct cactcggcgt cgggctggtt ctcgggggcg gcagcgcgga acgcgggcag
     3721 ctcggcacag gcaccctcga tcgtgaccag ggtcgggcag accccgacgt cgcagccgaa
     3781 gcggcgcgca ttggcgagct gcggcaccag gcaggcgtcg gcgattcccg ggaaatcgcc
     3841 gtggcagaag cggccggtcg ccgggtggcc ggcgaccatc tgctccatcg ccccgaggcc
     3901 gagctcgacc cagtgccggt accagcggtt gcgcgcgtcc tcgtcgatgt cgagctcgtc
     3961 cttgagatat tgcaggactc gcaggttgtt gagcgggtgg atgtcgcagg cgaccgccag
     4021 cgccagcgcg cgcacccgcg cgcgtgccgc gggatcggcc ggcaccaggc gcggctcggg
     4081 ccgggtctcc tccaggtagt tgatgatcgc cagcgactgc gtcaggacga cgccgtcatc
     4141 gtcgagcgtc ggcaccagcg cctgcgggtt gagcttgacg tagtcggggc ggaactgctc
     4201 gccgccgccg cgccgcagat ggacgaacac ctgctcgtag gcgaggccct tgaggttgag
     4261 cacgatgcgc acccggtagg ccgccgagga ccggtaatat ccgtacagct tcaacatcgt
     4321 ctcagtcctc ccccggcccc cggtagcgct cgaccacttg gtcgatggcg ccgaagatcg
     4381 aggcgccggc gtggtcgagc atctcgatcc gcacccggtc gccgaacttc atgaacggcg
     4441 ttatgggcgc gccgcgctcg atggtctcga tcatgcgcac ctcggcgatg cacgaccagc
     4501 cggcggcgcg gtcgcggttg ctgatcgtgc ccgagccgag gatggtgccg gcggtgagca
     4561 cgcgggtgcg cgcggcatgc gcgatcaggc gcgggaagtc gaactgcatg tccaccccgg
     4621 cattggcccg gcccacctgc ttgccgttga gacggacgat cagcggcagg ttgaccttgg
     4681 cgccgtccca ggccgcgccg agctcgtccg gggtcacagc gaccggcgag aacgcggtcg
     4741 cgggcttgcc ttggaagaag ccgaagccct tggcgagctc gaccgggatg aggccgcgca
     4801 gggacacgtc gttgaccagc atcaggagct tgacgtggcg cccggcctca gccggcatcg
     4861 cccccatcgg cacgtcgtcg aggatcgccg cgacctcgcc ctccaggtcg attccccagt
     4921 cctcgctcgc cgccaggatc gggtcgcacg ggccgatgaa cgcgtccgag ccgccctggt
     4981 acatcagcgg gtcgtcgagg aagctcgccg gcatctcggc gccgcgcgcg cggcgcacca
     5041 gctcgacgtg gttgaggtag gcgctggcgt cgacccattg ataggcgcgc ggcagcggcg
     5101 aggcgagcgc ggccgggtcg agcgcgaacg cgtcgtcgcg ccggccggcg tcgaggccgc
     5161 gcgcgaccgc ctcgagctcg ggcgcgaccg cctgccacgc ctcgatcgcg ctctgcaggg
     5221 tcggcgcgat gtggggcacg cgcaccgcga tgccgaggtc gcgcgcgacc accaccagcg
     5281 tcccgtcgcg gccgccctcc ttgaggctgc cgagcttcat tactccgcgg ccgcgtgcgc
     5341 gggcacccgc gcgccctgcc acgagcgcac gtagtccggg ttctcgaccc cggcgggcag
     5401 cgccgcgacc tcgagcgaat cgcgcgcatc gatcatcacc gccacctcgt cggtcgcggg
     5461 cttgggctgg accagcgcgt gctcgagcgc cttcgggtgc ggcccgtggg taaagcccga
     5521 cggatggaag gtgatcatgc cgcggtcgat gttgtcgcgg ctgaagaagt tgccgcggtg
     5581 gtagaagatg acctcgtcgt agtcgtcgtt gctgtggaag aacggcacct tgagggcgcc
     5641 cgggtcggtc tcgaacgggc gcggcacgaa ggtgcagacg acgaagcggc cggcgaggaa
     5701 ggtggtgtgc gccgacggcg gcaggtgcac gcggtggctc atgatcgggc ggatgtcgcg
     5761 ccagttgagc cgcaccggcg cgaggtcgcc gtgccaggac agcgcgtcga gcgggttgta
     5821 ggggaaggtc gcggtcgaga cggcgttccc cttcttgatc tcgacccgcc acgggcgctc
     5881 gtcctgctgg gcgcggaagg cgtcgtcgat gtgcggcgcc tcgagcaccg ccggatcgaa
     5941 gatcgcgtgc cgccccacca ggcccttctc gggcaggcgg aaagcgtcgt tggtcgcctc
     6001 gatcaacagc agctccgcgg cgccgtcgaa ctcggtccgc cacatggtgc cgcgcggcag
     6061 gaccaggtag tcgccgtcgc taaggtcgat gcggccgtag tcgcagaaca gccggccgcg
     6121 gccgtcgtgg acgaacagga tgtcgtcgcc gtcggcgttg cgggcgagcg cgggcatgtt
     6181 gccctcagcg cgccagaacc tgatccgtag gctcgcgttc gccagcacct cctgcgcctg
     6241 ccacggcatc tcggccgtgc ccgcgaggcg gttgaggtcg aacagccgcg gcctgagcgg
     6301 cccgctgaaa tcgctccagc cggtcggcgg atggcggtgg atcatctggg tcgagggccc
     6361 gaagaagcct tcgcggccga gctcgcgctc atagctgccc tcgggcaggt cgcagtgcgc
     6421 ctggcgccgg gcggtgccct cgagccaggg gtgcgagatc cagtgcttca tggcgacggt
     6481 ctcctgactg cgcatcggtc ggctccggct ccaacctata tcacagtcgg gtgagcgcga
     6541 ccgcgcgcga gtcgcgcccg aagttggacc ttggcggcga gagccctaat tcaactatcg
     6601 gcggctcgag ccaggcggcg ggcgctgata cggacgacgc gcgggtgtgc cgggggcggc
     6661 ggggtggccg aaccatcgga cgaaagtcgt aagctgatcg cactgatccg gtcgctccgg
     6721 caggcgttgg agcgcatcga gaccccggca tgcgacgccg cggcctcggc gttggcgact
     6781 gtcgcgaccg agatcgccgc ggccggtccc ggtgccgacc tgctggtcgt agccgatcag
     6841 cttcctgcgg cccgcgcggg ccgggcgcag gatcccgccg atggcgtgct cgatgtccga
     6901 aatcggcagt tccgcgacat cttgagtgcc tgtccgctgg gcgtggcggt gatctcgcgc
     6961 cgggggtgga aacgcctgtt catcaaccac tacctggtcg acatgttcaa ggcgccgtcg
     7021 atggaggcgt tccagcggca ggagctgatc gacagctggg tcgacaaggc ggagttcgcc
     7081 cgcgccgcgc gcaacatcgc cgccgacgtc gatcacgtcg atttcgaggc catgcgacgg
     7141 tgcttcgacg gctccatgat ctgggtgctg atgaactcgc taccgatcgt tttcgagggc
     7201 gagccggcgc gggttttctg gcacatcgac atcactgagc gcaagcgcat ggaggacgag
     7261 ctgcgcctgc tggcgacgac ggacgtgctc acgggcgtcg ccaaccgcgg gcatttcctc
     7321 gccaccgtgg accgcgaggt cgaacgccaa cggcgctacg gacccccgtg cgggctgctg
     7381 atgctggata tcgaccactt caagagcgtc aacgactcgt tcggccatgc ctgcggcgac
     7441 caggtgcttc gccacgtcgc ccggctgtgc ctcgagaaca tccgaggcat cgatacgctg
     7501 gggcgaatgg gcggcgaggg gttcgcggtg gtggtgcccg aaacggacga cgccggcgtg
     7561 ctgcgcctgg cggagcgcct tcgcctggcg gtggccggct cggtgctgga ctccgagacc
     7621 ggggccagcg tgcgcgtgac cttgagcgtc ggcgcgacac tccaccgctt gcacgacgac
     7681 gccgtcgagg tgaccttcgc gcgtgccgac aaggcgctct acgaggccaa gcatgccggg
     7741 cggaatcggg tcattttcta ttgatcccgg cgcgcgcccc cggcggacgg ccgggcgcgc
     7801 cggcgccttg ccgtgtagga tcacggccaa acgcggtctc gaccggatcg cccggtaaga
     7861 cgcgtcgctg aacggggcgt cggggacccg cggcttcgag ccgccggcgc ccgcccggcc
     7921 cgccgccatg aggattccgc gccgttggac cggtctcgcg ccgccacgat cctgagcctc
     7981 gccctgccga tcatcggcgg catggcgtcg cagaacctgt tcaacctggt cgacaccgcg
     8041 atggtcgggc ggctgggccc cgccgcgctc gccggcgtcg gcatcgcgag cttcgccaac
     8101 ttcacgtgcg tcgccgtggt cgtcggcctg tcggccgggg tgcaggcgat gtcggcgcgg
     8161 cgcaagggcg agggccggat cgcggagact gcggtgccgc tcaacggcgg gctgatcatc
     8221 gcgctcggcc tgtcgctgcc gctgtcggcg gcgctgatct gggcggtgcc gcgcctgttc
     8281 ccgctgctca atgccgatcc ggcggtgatc gccgagggcg tgccctattt cgaggccagg
     8341 ctggtcgcca tggccgcggt cggcatcaac ttcgcgttcc gcggcttctg gaacggcatc
     8401 ggccagtcgg gtgtctacct gcgcacgctc ctggtcatgc acgcgtgcaa catcgtgctc
     8461 gactacgtgc tgatcttcgg cgccttcggc gcgccgcgcc tcggcaccct cggcgccggc
     8521 atcggcaccg ccgtcgccac ctatgtcggt gccggcacct atctctggct cgcctggcgg
     8581 cgggcgcgcg ccaacggctt cctgcaccgc gtgcccaggc gcgcgaccat gctcaccatg
     8641 ctcaggctgg cgatcccgtc gtcgacccag cagctgttct tctcggccgg cttcacggtg
     8701 ctgttctgga tcgtcggcct ggtcggcacg gccgaggtcg cggccgccaa cgtgctgatc
     8761 aacgtcatgc tggtggcgat cctgcccggg ctcggcttcg gcctcgccgc ggcctcgctg
     8821 gtcggccagg cgctcggcgc gggcgatgcc gcggacgcgc gccgctgggg ctgggacgtg
     8881 gtcaaggtgg cggcggcgac cgtcgccgtg ctcggcctgc cgatgctcgc atttcccgat
     8941 gcgatcctcg gcgtcttttt gcgcgagccc gagacgctgg cgctggcgcg cctgccgctc
     9001 aggctggtcg gcgcgaccat cgccatcgag gcggtcgcca tggtgctgat gaacgccctc
     9061 ctcggcgcgg gcgcggcgcg ccaggtgatg gtggtgtcga tcaccctcca gtggctcgcc
     9121 ttcctgccgc tcgcctggct ggtcgggccc ggcctcggct acgggctgct cggcatctgg
     9181 gcgctccagg tggtctaccg ggcggtgcag gcgctggtgc tcaccggcct ctggcgccgc
     9241 ggccgctggg ccgagatcgt ggtgtaggcc gg
//
LOCUS       DASWHU010000060        10173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3348866, whole genome shotgun sequence.
ACCESSION   DASWHU010000060 DASWHU010000000
VERSION     DASWHU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3348866"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1060
                     /locus_tag="VGB88_02190"
     CDS             56..1060
                     /locus_tag="VGB88_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX9646278.1"
                     /translation="MGTTALDSEAAAGAHSARNDIAAARRVLDIEAAALRKLAESLDD
                     DFSRALDLLASARGRVVTTGMGKSGHVARKIAATLASTGTPAMFVHPGEASHGDLGMI
                     TRDDAVLALSNSGETAELDDIVEHAARVHIPLVAIVGRAGSTLAQRAAVALVLPSAPE
                     ACPNGMAPTTSTTMALALGDALAVALVDRSGFTGDAFRMLHPGGSLGARLRTVRDLMH
                     GGERMPLVGPDTAMSDALIEMTAKSFGCVGVVDATDGRLVGLITDGDLRRHMAPALLA
                     RRAGEVMTAAPKVIAPSHLAAEALWIMSNCAITSLFAVEDGRPVGILHLHDCLRAGIA
                     "
     gene            1057..1758
                     /locus_tag="VGB88_02195"
     CDS             1057..1758
                     /locus_tag="VGB88_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646279.1"
                     /translation="MSAQAPSSGRGEASRAHLVQRWRQRRLGGEALRAEVGYRRRLGL
                     LRLVMPLAVAGLLAVVVAWPIVTRPDTSFRLDFADIEFEVNGRDEMLSPHFVGADDKN
                     QPYSVTAEVAMRPATGGDLIYLIMPKADITLGGSDWLMISAEQGLYDRDGQTLDLVGS
                     VSLFSDSGLEVHTDKARFDLDGGGAESDVAVRSQGPWGWLDSTGFRYQPDGEVFYFTG
                     RPKLTLYPRAADGEG"
     gene            1761..2291
                     /locus_tag="VGB88_02200"
     CDS             1761..2291
                     /locus_tag="VGB88_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX9646280.1"
                     /translation="MLRPLLAAALLVAAAMPAPAQFEADSNAPVEITSDAMEWLNTQR
                     IAIARGNADAIQGRYHLHAEVLTAHLNQASGETLDRIRLIEAEGNVRLTTPQETASGK
                     TGVYDVERKVATLEGEVVLTQGDNVLRGERLVMNLETGRSTLVGAPEAAPVTAAGPGG
                     GRVKAIFTPDNQDPGQ"
     gene            2288..3091
                     /gene="lptB"
                     /locus_tag="VGB88_02205"
     CDS             2288..3091
                     /gene="lptB"
                     /locus_tag="VGB88_02205"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX9646281.1"
                     /translation="MTPPADDPRARAAGGPAAAASPRLVAESEGLVARHLGKRYRRRP
                     IVHDVSLSVRRGEAVGLLGPNGAGKTSCFYLITGLLRPDYGRISLDGHDISDLPMYRR
                     ARLGIGYLPQEASIFRGMTVEDNIRAVLEVAEPIQERRETMLDELLAEFSISHLRRAP
                     ALALSGGERRRVEIARALASQPSFMLLDEPLAGIDPIAVGEIRDLVAHLKHRGIGVLL
                     TEHNVRETLDMVDRAYILHDGRVLMEGEPSEIVAHEGVRQVYLGERFRL"
     gene            3105..4604
                     /gene="rpoN"
                     /locus_tag="VGB88_02210"
     CDS             3105..4604
                     /gene="rpoN"
                     /locus_tag="VGB88_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX9646282.1"
                     /translation="MVVGHRLEQRLGQALVMTPQLQQAIKLLQLSNLDLAAYVEQEVE
                     SNPLLERVPGDDTGPAEAGPAAGGEEPAGAPDPAEMTGSESLAGAGEAALGGDDGELY
                     DGGAPAPAERELGIEVYGRGGGTGFDDGGGFEDSLTRPRTLREHLEEQLDLDVADPTD
                     RVVCRHLIDALDEAGYVVGDCGQIAHALGCPPARVERALALMQRCEPAGVFARSLGEC
                     LALQLADQGRLTPPMQALVANLDLVAGHALDRLAELCAVAPEEVIEMVAELRRLDPKP
                     GLVFDSEPTQTVIADVIVRPHAGGWSVELNSDTLPKVLVNNRYYSLVGRAARTKQDRA
                     YLSERLQTANWLVKALEQRANTILKVATELVRQQDGFLRKGIMHLRPLTLRDIADAVS
                     MHESTISRVTTNKYMATPRGIFELKYLFSSAIPSSNGGASHSSEAIRHRIKELIDNEP
                     ADDVLSDDRLVAVLEASGIDIARRTVAKYRESMRIPSSVQRRRARAARI"
     gene            4737..5312
                     /gene="raiA"
                     /locus_tag="VGB88_02215"
     CDS             4737..5312
                     /gene="raiA"
                     /locus_tag="VGB88_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9646283.1"
                     /translation="MQLLVSGKQIDVGEALRQHVEDRLTAGVSKYFDRAIEAQVQFSK
                     ARHLYRADISAHVGRGVSLQAHAEADDIYPAFDAAAARIEKRLRRFKRRLNDHHGRKT
                     EPAPAETPARQLVLAPEGESEPAPEQEPEGPVTIAETTTSVHTLTVSEAVMRLELAEL
                     AVLVFRNSGHGRLNVVYRRDDGNIGWIDPTE"
     gene            5318..5785
                     /gene="ptsN"
                     /locus_tag="VGB88_02220"
     CDS             5318..5785
                     /gene="ptsN"
                     /locus_tag="VGB88_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX9646284.1"
                     /translation="MEIERLLSPDAVIANLKATSKKQALQELAKHAAGLTGLAERRIF
                     DVLLERERLGSTGVGHGVAIPHARMAELDRLFGLFARLEAPIDFDAVDEQPVDLIFLL
                     LAPESAGADHLKALARISRLLRDKASCAKLRGADNADALYAVLVEPAASHDAA"
     gene            5839..6294
                     /locus_tag="VGB88_02225"
     CDS             5839..6294
                     /locus_tag="VGB88_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9646285.1"
                     /translation="MPTVHASCVALDGAGVLLRGRPGSGKSDLALRLVDAGARLVADD
                     RVRVRAAAGRLLASAPAATAGLIEVRGLGILRLAHQGEVALALVVDLDAGAAPERLPA
                     PSACEIAGIVLPRIVLDGFAASAAARIRLAVGVLAAGGSIAGALGDLPP"
     gene            6291..7157
                     /gene="rapZ"
                     /locus_tag="VGB88_02230"
     CDS             6291..7157
                     /gene="rapZ"
                     /locus_tag="VGB88_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9646286.1"
                     /translation="MSPRARLRLVTGLSGAGKSTALRALEDLGYEVVDNLPIKLVANL
                     LGDAPQPGTALAVGVDARTRDFDSRTLEEALDALAARPDLETTLLFLDCDDEVLMRRY
                     TETRRRHPLADDRPVSAGIRHERRLMAPVRARADHAIDTSEIAAAELGRILAGHYGLE
                     ARPGLRLFVTSFGFRHGLPREADLVLDVRFLRNPHYVAALRPLTGLDARVAAYVADDP
                     EFAPFFDRVGALLVPLLPRYAAEGKAYLTIAIGCTGGRHRSVVVAARLAQRLGEAGIA
                     AALHHRDLGRER"
     gene            7230..7637
                     /locus_tag="VGB88_02235"
     CDS             7230..7637
                     /locus_tag="VGB88_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9646287.1"
                     /translation="MIGIVLVTHGRLAAEFVAATEHVVGPQQAIRSVCIGPDDDMEQR
                     RADIVDAVAAVDEGDGVVVLTDMFGGTPSNLAISIMDRSKVEVIAGINLPMLIKLASV
                     RADRSLDEAVACAQDAGRKYINVASRLLADEPK"
     gene            7634..7936
                     /locus_tag="VGB88_02240"
     CDS             7634..7936
                     /locus_tag="VGB88_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9646288.1"
                     /translation="MMRRVTRAATRRVRARTVTVVNQRGLHARAAARFVKLAEQFDAE
                     VTVRRDATTVSGRSIMGLMMLAAGPGCALELRASGRDARDALEALGRLIAGRFDED"
     gene            7926..9707
                     /gene="ptsP"
                     /locus_tag="VGB88_02245"
     CDS             7926..9707
                     /gene="ptsP"
                     /locus_tag="VGB88_02245"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX9646289.1"
                     /translation="MRTELSRQARASRTHSGIGASAGVCIGPARLIEASGLDVAERTL
                     PQRAVAAEQERFQAAVERARAEVEGLAQRAGPLPESARQELAFLLEAQLQMLAGSRLV
                     RGVYRRIAETRVNAEAAVKTELSAIAEAFAAMHDPYLSARAQDVREVALRLIRQLTAE
                     PERGFADLPEGAVLIAEELTPADIALLARGRIAGFATEFGGPQGHTAIMARSLALPAV
                     VGVPALMHRVKAGETVVIDGTRGQVIVDPDAATVAAVRRRRSARKRDQRVLAKLRDLP
                     AETRDGQVISLQANLELPAELAQATAAGAEGIGLLRTEFLYMNRDTVPDADEQYAALS
                     RIVTAMGGRPVTVRTLDIGTDKLADALARDAATDVGANPALGLRAIRLSLRHRKLLED
                     QLEAMLRAAAHGPVRILLPMITVGSEVRQTRAVLRRVARRLARRGVALPESLPPLGAM
                     VEIPAAALAAEALVRDCDFISIGTNDLTMYTLAVDRGDEHVAELYDPLHPAVLRLVQY
                     AAEAAKRARVPVNLCGEIAGDVRYTALLIGLGLEDLSMAAASLPAVKRRIRALDAGKA
                     RSLAAQVMRRNEARQIATLIDAFNDAL"
BASE COUNT         1407 a         3743 c         3712 g         1311 t
ORIGIN      
        1 acatgcaaga acggggccgc ttcggcggcg ggcgcgggcg gcacgattga gccaggtggg
       61 gacgacggca ctggacagcg aggcggcggc cggcgcgcat tcggcgcgta acgacatcgc
      121 cgcggcgcgg cgcgtgctcg acatcgaggc cgcggcgctg cgcaagctcg ccgaaagcct
      181 cgacgacgat ttctcgcgcg ccctcgacct gctggcgtcg gcgcgcgggc gcgtggtcac
      241 caccggcatg ggcaagtcgg gccacgtcgc gcgcaagatc gcggccacgc tcgcctcgac
      301 cggaaccccg gcgatgttcg tccaccccgg cgaggcgagc cacggcgacc tcggcatgat
      361 cacccgcgac gacgcggtgc tggcgctgtc gaactcgggc gagaccgccg agctcgacga
      421 catcgtcgag cacgcggcgc gcgtccacat cccgctggtg gcgatcgtcg ggcgcgccgg
      481 cagcacgctg gctcagcgcg cggcggtggc gctggtgctg cctagcgcgc ccgaagcctg
      541 ccccaacggc atggcgccga ccacctcgac caccatggcg ctggcgctgg gcgacgcgct
      601 ggcggtggcg ctggtcgacc gctcgggctt caccggcgat gcgttccgga tgctccatcc
      661 gggcggcagc ctgggcgcgc ggctgcgcac cgtgcgcgac ctgatgcatg gcggcgagcg
      721 catgccgctg gtcggcccgg acacggcgat gtcggacgcc ctgatcgaga tgaccgccaa
      781 gagcttcggc tgcgtcggcg tggtcgatgc aacggacggg cggctggtcg ggctgatcac
      841 cgacggcgac ctcaggcggc acatggcgcc cgcgctgctg gcgcgccggg ccggcgaggt
      901 catgaccgcc gcgcccaagg tgatcgcgcc cagccacctc gccgccgagg cgctgtggat
      961 catgagcaac tgcgccatca cctccctgtt cgcggtcgag gacggccgcc cggtcggcat
     1021 cctgcacctg cacgactgcc tgcgcgcggg gatcgcgtga gcgcgcaggc gccgagcagc
     1081 gggcggggcg aggcgtcgcg cgcgcacctg gtccagcgct ggcgccagcg ccggctcggc
     1141 ggcgaggcgc tgcgtgccga ggtcggctac cggcgccgtc tcgggctgtt gcgcctggtc
     1201 atgccgctgg cggtggcggg tctgctggcg gtggtcgtcg cctggccgat cgtgactcgt
     1261 cccgacacca gcttccggct cgacttcgcc gacatcgagt tcgaggtcaa cggccgcgac
     1321 gagatgctga gcccgcactt cgtcggcgcc gatgacaaga accagcccta ttcggtgacc
     1381 gccgaggtgg cgatgcgccc ggccaccggc ggcgacctga tctacctgat catgcccaag
     1441 gccgacatca cgctcggcgg cagcgactgg ctgatgatca gcgccgagca gggcctgtac
     1501 gaccgcgacg gccagacctt ggacctggtc ggctcggtca gcctgttctc cgacagcggc
     1561 ttggaggtcc ataccgacaa ggcgcgcttc gacctcgacg gcggcggcgc cgagagcgac
     1621 gtggcggtgc gcagccaggg gccgtggggc tggctcgact cgaccggctt ccgctaccag
     1681 cccgacggcg aggtgttcta tttcaccggc cggccgaagc tgacgctcta tccgcgcgcc
     1741 gccgacggcg agggctagcg atgctgcgcc ccctgcttgc ggccgcgctg ctggtcgccg
     1801 cggcgatgcc cgccccggcc cagttcgagg ccgattcgaa cgccccggtc gagatcacct
     1861 cggacgccat ggagtggctg aatacgcagc gcatcgccat cgcccgcggc aatgccgacg
     1921 ccatccaggg ccgctaccac ctgcatgccg aggtgctcac cgcccatctc aaccaggcca
     1981 gcggcgaaac gctcgaccgc atccgcctga tcgaggccga gggcaacgtc cgcttgacga
     2041 caccgcagga gacggcgagc ggcaagaccg gcgtctacga cgtcgagcgc aaggtggcca
     2101 ccctcgaggg cgaggtggtg ctgacccagg gcgacaacgt gctgcgcggc gagcgcctgg
     2161 tcatgaacct ggagaccggc cgctcgacgc tggtcggggc gcccgaggcc gcgccggtga
     2221 ccgcggccgg gccgggcggc ggccgggtta aggcgatctt caccccggac aaccaggatc
     2281 ccggccaatg accccgcccg ccgacgaccc gcgcgcgcgc gccgccggcg gaccggcagc
     2341 ggccgcctcg ccgcgcctgg tggcggagag cgagggcctg gtcgcgcgcc atctcggcaa
     2401 gcgctaccgg cgccggccga tcgtgcacga cgtgagcctg tcggtgcgcc gcggcgaggc
     2461 ggtcggcctg ctcggcccga acggcgccgg caagaccagc tgcttctacc tgatcaccgg
     2521 cctgctgcgc ccggactacg gccgcatcag cctcgacggc cacgacatca gcgacctgcc
     2581 catgtaccgg cgtgcccgcc tcggcatcgg ctatttgccg caggaggcgt cgatcttccg
     2641 cggcatgacg gtcgaggaca acatccgcgc cgtgctcgag gtggcggagc cgatccagga
     2701 gcggcgcgag accatgctcg acgagctgct cgccgagttc tcgatcagcc acctgcgccg
     2761 ggcgccggcg ctggcgctgt cgggcggcga gcgccggcgg gtcgagatcg cgcgcgcgct
     2821 cgcctcgcag cccagcttca tgctgctcga cgagccgctg gccggcatcg acccgatcgc
     2881 ggtcggcgag atcagggacc tggtcgccca tctcaagcac cgcggcatcg gcgtgctgct
     2941 caccgagcac aacgtgcgcg agacgctcga catggtcgac cgcgcctaca tcctgcacga
     3001 cggccgcgtc ctgatggagg gcgagccgag cgagatcgtc gcccacgagg gcgtccgcca
     3061 ggtctatctg ggcgagcgct tccgtctctg agcggagacc ggcgatggtg gtcgggcaca
     3121 gactcgagca acgtctcggc caggcgctgg tgatgacgcc gcagctgcag caggcgatca
     3181 agctgctgca gctgtcgaac ctcgatctgg ccgcctatgt cgagcaggag gtcgaaagca
     3241 accccttgct cgagcgcgtg ccgggcgacg acaccgggcc ggcggaggcg ggcccggcgg
     3301 ccggcggcga ggagccggcg ggggcgcccg acccggccga gatgaccggt tccgagtcgc
     3361 ttgccggggc cggcgaggcg gcgctcggtg gcgacgacgg cgagctctac gacggcggcg
     3421 cgccggcgcc ggccgagcgc gagctcggga tcgaggtcta cggccgcggc ggcggcaccg
     3481 gcttcgacga cggcggcggc ttcgaggaca gcctgacccg gcccaggacc ctgcgcgagc
     3541 atctcgagga gcagctcgac ctcgacgtcg ccgacccgac cgatcgggtg gtctgccgcc
     3601 atctgatcga cgccctcgac gaggccggat acgtcgtcgg cgactgcggg cagatcgcgc
     3661 acgcgctcgg ctgcccgccg gcgcgggtcg agcgggcgct ggcgctgatg cagcgctgcg
     3721 agccggccgg ggtgttcgcg cgctcgctgg gcgaatgcct ggcgctgcag ctcgccgacc
     3781 aaggccggct gacgccgccg atgcaggcgc tggtcgccaa cctcgacctg gtcgccggcc
     3841 atgccctcga ccggctcgcc gagctgtgcg cggtcgcgcc ggaagaggtg atcgaaatgg
     3901 tcgccgagct caggcgcctc gaccccaagc ccggcctggt gttcgacagc gagccgaccc
     3961 agaccgtgat cgccgacgtc atcgtccgcc cccacgccgg cggctggagc gtcgagctga
     4021 actcggacac cttgccgaag gtgctggtca acaaccgcta ttactccctg gtcgggcgcg
     4081 cggcgcgcac caagcaggac cgcgcctatc tcagcgagcg gctgcagacc gccaactggc
     4141 tggtcaaggc gctggaacag cgcgccaaca ccatcctcaa ggtggcgacc gagctggtcc
     4201 gccagcagga cgggttcctg cgcaagggca tcatgcacct caggccgctg accctgcgcg
     4261 acatcgccga cgcggtcagc atgcacgaga gcacgatcag ccgggttacg accaacaagt
     4321 acatggccac gccgcggggc atcttcgagc tcaagtacct gttctcgtcg gcaatcccca
     4381 gtagcaacgg cggcgccagc cactcctcgg aggcgattcg ccatcgcatc aaggagctga
     4441 tcgacaacga gccggccgac gacgtgctgt ccgacgaccg cctggtggcg gtgctcgagg
     4501 cgagcggcat cgacatcgcc cggcgcaccg tcgccaagta ccgcgagtcg atgaggatcc
     4561 cgtcatcggt ccagcgccgc cgggcgcggg ccgcccgcat ctgaccgcgc gccggcggcg
     4621 ccggcggcgt tgacaggggc gggggcgtta actaaggtcc gcccccttcg cgcgggcggc
     4681 cgggcgggac ctgagcgccg ccgccgaccc ggcccatcat cggacaatcg cgccgcatgc
     4741 agctgttggt aagtggcaag caaatcgacg tgggcgaggc gttgcgccaa catgtcgagg
     4801 atcggctgac cgcgggcgtg agcaagtatt tcgatcgggc gatcgaggcg caggtgcagt
     4861 tctcgaaggc gcggcacctg taccgggcgg acatctcggc ccatgtcgga cgcggcgtct
     4921 cgctgcaggc ccacgccgag gccgacgaca tctatccggc gttcgatgcc gccgccgcca
     4981 ggatcgagaa gcggctgcgc cggttcaagc gtcggctcaa cgaccatcac gggcgcaaga
     5041 ccgagccggc gcctgcggag accccggccc gccagctggt gctcgcgccc gaaggcgaga
     5101 gcgagcccgc gcccgagcag gagcccgagg gcccggtgac catcgccgag acgaccacca
     5161 gcgtgcatac gctcacggtc agcgaggcgg tgatgcgcct cgagctggcc gagcttgcgg
     5221 tgctggtgtt ccgcaacagc ggccacgggc gcctcaacgt ggtctaccgg cgcgacgacg
     5281 gcaacatcgg ttggatcgac ccgacggagt agcagcgatg gagatcgagc gcctgctgtc
     5341 accggacgcg gtgatcgcca acctcaaggc gaccagcaag aagcaggccc tgcaggagct
     5401 ggccaagcac gcggccgggc ttaccgggct ggccgaacgg cgcatcttcg acgtgctgct
     5461 cgagcgcgag cggctgggca gcaccggcgt cggccacggc gtcgccatcc cgcatgcgcg
     5521 catggccgag ctcgaccgac tgttcggtct gttcgcccgc ctggaggcgc cgatcgactt
     5581 cgacgcggtc gacgagcagc cggtggacct gatcttcctg ctgctggcgc cggaatcggc
     5641 cggcgccgat cacctcaagg cgctggcgcg catctcgcgg ctgctgcgcg acaaggcgag
     5701 ctgcgccaag ctgcgcggcg ccgacaacgc cgatgcgctg tacgcggtcc tggtcgagcc
     5761 ggccgcgagc cacgacgccg cctgagtgcc cgccgggcgc gattgacagc gcccgggttc
     5821 gaccccagcc tcttggccat gccaaccgtg cacgcgagct gcgtcgcgct cgacggcgcc
     5881 ggcgtgctgc tgcgcggccg gccgggcagc ggcaagtcgg acctggcgct gaggctggtc
     5941 gacgccggcg cgcggctggt cgccgacgac cgggtgcggg tgcgcgccgc cgccggccgg
     6001 ctgctggcct cggcgccggc cgcgaccgcc ggcctgatcg aggtccgcgg cctcggcatc
     6061 ctgcgcctgg cgcatcaggg ggaggtggcg ctggcgctgg tcgtcgatct cgatgccggc
     6121 gccgcgcccg agcgcctgcc ggcgccctcg gcgtgcgaga tcgccggcat cgtgttgccg
     6181 cgcatcgtgc tcgatgggtt cgccgcctcg gcggcggcgc gcatccggct cgcggttggc
     6241 gtgctcgccg ccggcggcag catcgccggc gcgctcggcg acctgccgcc gtgagcccgc
     6301 gcgcgcgcct gcggctggtc accgggctgt cgggggcggg caagagcacc gcgctcaggg
     6361 cgctcgagga cctcggctac gaggtggtcg acaacctgcc gatcaagctg gtcgccaacc
     6421 tgctcggcga tgcgccgcag cccggcaccg cgctcgcggt cggggtcgat gcccgcaccc
     6481 gcgacttcga cagccgcacg ctggaggagg cgctcgatgc gctggccgcg cgccccgacc
     6541 tcgagaccac gctcctgttc ctcgactgcg acgacgaggt gctgatgcgc cgctacaccg
     6601 agacccggcg ccgccacccg ctggccgacg accgcccggt cagcgccggc atccgccacg
     6661 agcgccggct gatggcgccg gtgcgcgcac gcgccgacca cgccatcgac acctcggaga
     6721 tcgcggcggc cgagctcggc cgcatcctcg ccggccatta cgggctcgag gcgcgccccg
     6781 gcttgcgcct gttcgtcacc tcgttcggct ttcgccacgg cctgccgcgc gaggccgacc
     6841 tggtgctcga cgtgcgcttc ctgcgcaacc cgcactacgt cgccgcctta aggccgctga
     6901 ccgggctcga tgcgcgggtc gccgcctatg tcgcggacga cccagagttc gcgccgttct
     6961 tcgaccgggt cggcgcgctg ctcgtgccgc tgctgccgcg ctatgccgcc gagggcaagg
     7021 cgtacctgac catcgccatc ggctgcaccg gcggccgcca ccgctcggtg gtggtcgccg
     7081 cccgcctcgc ccagcgcctc ggcgaggccg ggatcgccgc cgccctccac caccgcgacc
     7141 ttggccgcga gcgctgagcc cttggcaagc ggcggcgaat gtcgtgaagt gcggcccgcg
     7201 ccccggcgcc cggccaagga gacgctcaca tgatcggcat cgtgctcgtc acccacggcc
     7261 gcctggctgc ggagttcgtc gccgcgaccg agcacgtggt cgggccgcag caggcgatcc
     7321 gcagcgtctg catcggcccc gacgacgaca tggagcagcg gcgcgccgac atcgtggacg
     7381 cagtcgcggc ggtggacgag ggcgacggcg tggtggtgct gaccgacatg ttcggcggca
     7441 ccccgtcgaa cctggcgatc tcgatcatgg accgctccaa ggtcgaggta atcgccggga
     7501 tcaacctgcc gatgctgatc aagctcgcca gcgtgcgcgc ggaccggagc ctggacgagg
     7561 cggtcgcctg cgcccaggac gccgggcgca agtacatcaa cgtcgccagc cggctgctgg
     7621 cggacgagcc caaatgatgc ggcgggtgac gcgcgccgcc acccgccggg tccgtgcgcg
     7681 caccgtcacc gtggtcaacc agcgcggcct gcacgcgcgc gccgcggcgc ggttcgtcaa
     7741 gctggccgag cagttcgacg ccgaggtcac ggtccgccgc gacgccacca ccgtctccgg
     7801 gcgctcgatc atgggcctga tgatgctggc ggcgggaccg ggctgcgcgc tcgagctgcg
     7861 cgccagcggg cgcgacgcgc gggacgcgct cgaggcgctc ggccgcctga tcgccggccg
     7921 cttcgatgag gactgagctg tcccggcagg cgcgcgcgag ccgcacccat tccggcatcg
     7981 gcgcctccgc cggggtgtgc atcggcccgg cgcggctgat cgaggcgagc ggcctcgacg
     8041 tcgccgagcg caccttgccc cagcgcgcgg tggctgccga gcaggagcgc ttccaggccg
     8101 cggtcgagcg cgcccgcgcc gaggtcgagg gcctggccca gcgcgccggc ccgctgcccg
     8161 agtcggcgcg ccaggagctt gccttcctgc tcgaggccca gctgcagatg ctggccggct
     8221 cgcgcctggt gcgcggcgtc taccggcgta tcgccgagac ccgggtcaac gccgaggcgg
     8281 cggtcaagac cgagctcagc gccatcgccg aggcgttcgc ggcgatgcac gacccgtatc
     8341 tgtcggcccg cgcccaggac gtgcgcgagg tggcgctgcg cctgatccgc cagcttaccg
     8401 cggaacccga gcgcggcttc gcggacctcc ccgagggcgc ggtgctgatc gccgaggagc
     8461 tgaccccggc cgacatcgcg ctgctcgcgc gcggccgcat cgccggcttc gccaccgagt
     8521 tcggcggccc gcagggccac accgcgatca tggcgcgctc gctggcgctg ccggcggtgg
     8581 tcggcgtgcc cgcgctgatg caccgggtca aggccggcga gacggtggtc atcgacggca
     8641 cccgcggcca ggtgatcgtc gaccccgatg cggccaccgt cgccgcggtg cgccgccggc
     8701 gcagcgcgcg caagcgcgac cagcgcgtgc tcgccaagct ccgcgacctg ccggcggaaa
     8761 cccgcgacgg gcaggtgatc tcgctgcagg cgaacctgga gctgccggcg gagctggcgc
     8821 aggcgaccgc cgccggcgcc gaaggcatcg gcctgctgag gaccgagttc ctgtacatga
     8881 accgcgacac cgtgcccgac gccgacgagc agtacgcggc gctcagccgt atcgtcaccg
     8941 ccatgggcgg ccggccggtg accgtgcgca ccctcgacat cggcaccgac aagctggccg
     9001 atgcgctggc gcgcgacgcc gccaccgacg tcggcgccaa cccggcgctc ggcctgcgcg
     9061 cgatccggct gtcgctcagg caccgcaagc tgcttgaaga tcagctcgag gcgatgctgc
     9121 gcgccgccgc ccacggcccg gtgcgcatcc tgttgccgat gatcacggtc ggcagcgagg
     9181 tgcgccagac ccgcgccgtg ttgcggcgcg tcgcccgccg gctcgcccgc cgcggcgtcg
     9241 ccctgcccga gtccctgccg ccgctcggcg cgatggtcga gattcccgcc gccgcgctcg
     9301 ccgccgaggc gctggtgcgc gactgcgact tcatctcgat cggcaccaac gacctgacca
     9361 tgtacaccct cgccgtcgac cgcggcgacg agcatgtcgc cgagctctac gacccgctcc
     9421 acccggcggt gctgcggctg gtccagtacg ccgccgaggc cgcgaagcgc gcgcgggtgc
     9481 cggtcaatct gtgcggcgag atcgccggcg acgtgcgcta caccgcgctc ctgatcgggc
     9541 tcgggctcga ggacctgtcg atggccgcgg ccagcctgcc ggcggtcaag cggcgcatcc
     9601 gcgcgctcga cgccggcaag gcgcggagcc tcgccgcgca ggtcatgcgc cgcaacgaag
     9661 cgcgccagat cgcgaccctg atcgacgcct tcaacgacgc cttgtaggac ctgcctccgg
     9721 tggacgggta catagctttc agccggtgaa cgtacgaatt ggccgggaat agctctgatc
     9781 cgacgggatt tggtgggatc ggggacggcg gcggcgggcg tcagtgcgac ggagtggaag
     9841 ccacgtcgaa ggccgaataa agggccgcca aacgggcctt gcacgacggg cagatgcggt
     9901 tgccggcgtg ctcagagtgg aagcgccgcc ggccgttaca ccacgggccg aggcaggccc
     9961 ggctcgcggg ccggttgcgg gggtcggacc accagcggcg catcgcggcg tagaagccct
    10021 cgccttcgtc ccagggattc ggagggaccg tcgtcatcgc ctcggcagct tggcgaagcg
    10081 gccgccgcgg cgggcgtcgg cctcgatcgc gtccatggtg gcgcgggcga tgtcggcggc
    10141 ggcggcgcgc aggcgcgcgg cctcgccggg cgc
//
LOCUS       DASWHU010000061         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1135560, whole genome shotgun sequence.
ACCESSION   DASWHU010000061 DASWHU010000000
VERSION     DASWHU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135560"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGB88_02250"
     CDS             <1..230
                     /locus_tag="VGB88_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646290.1"
                     /translation="DRKLTVTYVEGEPTTRKVGMQEITVSPDAKVVGFFTADRGALAT
                     GQKVFVLAVDTDAGIAVPAMVIGRNGVEPPM"
     gene            329..913
                     /gene="pyrE"
                     /locus_tag="VGB88_02255"
     CDS             329..913
                     /gene="pyrE"
                     /locus_tag="VGB88_02255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9646291.1"
                     /translation="MPVAAATKQSAAGGARARLADIVRARSLITGRAVTLTSGATSGF
                     YFDMKQTTFDAEGATLIGDLILGALAGTEVDYVAGLEMGALPVVMAVVMQSQARGRPV
                     AGFCVRKEAKGHGTKRLIERDFAAGAKVVVVDDVTTTGGSVLKAAAAVRAAGGVVDTV
                     VTVVDRLEGAAANLAAEGLTLIPLLTARDFDLAA"
     gene            966..1595
                     /locus_tag="VGB88_02260"
     CDS             966..1595
                     /locus_tag="VGB88_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646292.1"
                     /translation="MTPLHHLLAPVLAAAMACVPAPAHSQGADREQADPGGDSLMMAA
                     PEGWVPVGQQRTDQLYELAYLPEGQSLEDWQASLQVQIFFNLAKQRPDLTPADFTENM
                     VRVWSTSCEEARASPISSFHERGYPAAVRMLTCPRSRGGETGSVSMIKVMQGRASMYL
                     LERAWRGPVFAAGDMPVAQETLDEWAEFLAATSLCNPDNAEAPCGGAPR"
     gene            1592..>1963
                     /locus_tag="VGB88_02265"
     CDS             1592..>1963
                     /locus_tag="VGB88_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646293.1"
                     /translation="MRLCVLAAALALAACFESEEERAVEAYQGHDYAAAHALGVRLAA
                     GGNARGHELLALMAAQGLGAPVDLDAALDHIDRAVALDPDYASSREALRDLIEARGEA
                     ARDAFEAGEFARAGALAAPLAA"
BASE COUNT          263 a          701 c          746 g          253 t
ORIGIN      
        1 aggaccgcaa gctgacggtg acctatgtcg agggcgagcc gaccacgcgc aaggtcggca
       61 tgcaggagat caccgtctcg cccgacgcca aggtggtcgg cttcttcacc gccgaccgcg
      121 gcgcgctggc caccggccag aaggtgttcg tgctggcggt cgacaccgac gccggcatcg
      181 ccgtgccggc gatggtgatc gggcgcaacg gcgtcgagcc gccgatgtag gccgcgcgcc
      241 acgcgaaccg ggccgtggcc agacgcctgc gcgggtgcta gatagccccg cggcgcgagg
      301 ggctgcgccc aagcgcgagg agcggcacat gccggtagcg gcggcaacca agcaatcggc
      361 ggcagggggc gcgcgcgcgc ggcttgccga catcgtccgc gcgcgctcgc tgattaccgg
      421 ccgggcggtg acgctcacct ccggcgcgac cagcggcttc tacttcgaca tgaagcaaac
      481 caccttcgat gccgagggcg cgaccctgat cggcgatctc atcctcggcg cgctcgccgg
      541 gacagaggtg gactacgttg ccggcctcga gatgggcgcg ctgccggtgg tgatggcggt
      601 ggtgatgcag agccaggcgc ggggccgccc ggtcgccggc ttctgcgtgc gcaaggaggc
      661 caagggccac ggcaccaagc gcctgatcga gcgcgacttc gcggcggggg cgaaggtggt
      721 ggtggtcgac gacgtcacca ccaccggcgg ctcggtgctc aaggcggcgg cggcggtgcg
      781 cgcggcgggg ggcgtggtcg acaccgtggt caccgtggtc gaccggctgg aaggggccgc
      841 ggccaacctc gccgccgagg gtctgacgct gatcccgctg ctcaccgcgc gcgacttcga
      901 tctcgcggcc tgagagcgcg cgcacgggtt ccgcgcgcgc ccggtttcgg tataggctgg
      961 gcgcgatgac gccgctgcac cacctgctcg ccccggtcct cgccgccgcc atggcgtgcg
     1021 tgccggcccc cgcccacagc cagggcgccg accgcgagca ggccgacccc ggcggcgaca
     1081 gcctgatgat ggccgcgccc gagggctggg tgccggtcgg ccagcagcgc accgaccagc
     1141 tctacgagct cgcctacctg cccgagggcc agagcctcga ggactggcag gcctcgctgc
     1201 aggtccagat cttcttcaac ctggccaagc agcgccccga tctgaccccc gccgacttca
     1261 ccgagaacat ggtgcgcgtc tggagcacgt cgtgcgagga ggcgcgcgcg agcccgatct
     1321 cgtcgttcca cgagcgcggc tacccggcgg cggtgcgcat gctgacctgc ccgcgcagcc
     1381 gcggcggcga gaccggctcg gtgtcgatga tcaaggtcat gcaggggcgg gcgagcatgt
     1441 atctgctcga acgggcgtgg cgcggcccgg tgttcgcggc cggcgacatg ccggtggcgc
     1501 aggagacgct cgacgaatgg gccgagttcc tggcggcgac ctcgctgtgc aacccggaca
     1561 acgccgaggc gccctgcggc ggcgcgccca ggtgaggctg tgcgtgctgg cggcggcgct
     1621 ggcgctcgcc gcctgtttcg agtccgagga ggagcgcgcg gtcgaggcct atcagggcca
     1681 cgactacgct gcggcgcacg cgctcggggt gcgtcttgcc gccggcggca acgcgcgcgg
     1741 ccacgagctg ctggcgctga tggccgccca ggggctcggc gcgccggtcg atctcgacgc
     1801 ggcgcttgac catatcgatc gagcggtggc cctcgatccc gactacgcct cgagccgcga
     1861 ggcgctgcgc gacctgatcg aagcgcgcgg cgaggctgcc cgcgacgcgt tcgaggccgg
     1921 cgagttcgcg cgcgccggcg ccctcgccgc gccgctcgcc gcc
//
LOCUS       DASWHU010000062        15740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1136301, whole genome shotgun sequence.
ACCESSION   DASWHU010000062 DASWHU010000000
VERSION     DASWHU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15740
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136301"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGB88_02270"
     CDS             <1..309
                     /locus_tag="VGB88_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX9646294.1"
                     /translation="DVILDGGVRRGTDVLKAVALGAKACMAGRPYLYGLAAGGEAGVD
                     RALGLLKDEIRRDMALLGVRDLGQLGPARIRARPEALSHPPARAQAAPPPRLDVVKSG
                     "
     gene            complement(521..1420)
                     /locus_tag="VGB88_02275"
     CDS             complement(521..1420)
                     /locus_tag="VGB88_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9646295.1"
                     /translation="MSKVYLFSVGPDTPPLMTVDPGAEFTVEVEGAFDDIDDITAVPT
                     PFTSESEGHPLAPITGPIEVRGAEPGDAVVIELRRITPHGVGRNAILRAFGVLREEFP
                     DPRIIACPIRDGMAWFGDRIPVAIEPNLGTVSTMPPAGYKPAYAGAYGGDLDQRHVEA
                     GSRLHLPVLMPGAGVFFADPHAAIKDGIVSGTGIECAVTLEASIELDKGRAVARPLIE
                     RADSIQVVGFGETVEAATEDATRHAVDYLARTTDLGREQAYMLLGAIGDLRVGTSPRP
                     VMAARLIIPRAPLAAAGWTGAAG"
     gene            1598..2053
                     /locus_tag="VGB88_02280"
     CDS             1598..2053
                     /locus_tag="VGB88_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646296.1"
                     /translation="MVSNQRQARTGWRLAAFGALAFALAGCLMSRAYDQPMGQDLTVA
                     KRNVMISGYENRVEVEGETFVFEGPLAHEQHAYAVRYMGLDSRGNPVLQVIATPLART
                     DVPVELAETDRMHLSGYGIPPITVQVVNATDELLTYRLRADGPFKATVQ"
     gene            2099..2386
                     /locus_tag="VGB88_02285"
     CDS             2099..2386
                     /locus_tag="VGB88_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646297.1"
                     /translation="MVQNSTHGTLEVISFGLAGGVCAALITLVLGFSAMVFGWGVAAV
                     QVLSSLLVGFGPSFVGLAAGVVWAFFDGFVASLLLAWLYNRFVLRRRRHII"
     gene            complement(2400..3944)
                     /locus_tag="VGB88_02290"
     CDS             complement(2400..3944)
                     /locus_tag="VGB88_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9646298.1"
                     /translation="MIPNSAAIAAAAGRATVGGLLRDRAQSQPDRIAVEDRRRSWTYR
                     ELNRRVNRLAHALAARGIGRGDRVAILSENRAEYLELEFAAAKLGAIVPALNWRLGAD
                     ELDYCVALTTPKAALVSPRHAAALAALDARPATVVELGEPYEAMLAAAPETEPVEVAD
                     PEDGLVILYTSGTTGRPKGAVVSQRAFISRVLVFCADYGIGRDELFPAWAPMFHMAST
                     DLAIGTLLIGGRVALIDGFDLDHIAEVLAGHPVGWLILMPGVIDRLLERHRAAPLAVK
                     GVRMTGAMADLVPRHVIAEVTRALAAPYLNSFGATETGIPPASGALIPPGEVPERLSK
                     RQSSMCELRLVDEADNDVPDGTPGEVAIRGPTLFSGYWNAPEANARDFRGGWFHMGDV
                     FVRNADGSLDFVDRAKYLIKTGGENVYPAEIEQVLLADPRVADAVVVRRADARWGEVP
                     VAVVARRDDSLGADELMARCRERLAGYKRPKEIRFVALDELPRSTTGKIQRHEIEARL
                     AAEDAG"
     gene            4058..4132
                     /gene="pqqA"
                     /locus_tag="VGB88_02295"
     CDS             4058..4132
                     /gene="pqqA"
                     /locus_tag="VGB88_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEX9646299.1"
                     /translation="MWSTPVVREIAVGLEINCYACADL"
     gene            4205..5134
                     /gene="pqqB"
                     /locus_tag="VGB88_02300"
     CDS             4205..5134
                     /gene="pqqB"
                     /locus_tag="VGB88_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEX9646300.1"
                     /translation="MMRIRVLGSAAGGGFPQWNCNHPNSRRARTGDPAAKRRTQSSIA
                     ISADGRRWFLCNASPDLRQQILDNDVLHPRNGLRNSPIQGVVLTNADVDHIAGLLTLR
                     ESQPLAIYGTARVLAVLAANNVFNVLNPELVARRPLALDAPNRLVCPDGGDSGLEVVP
                     FPVPGKVALWLEDASKGANFGTVAEDTVALEIREDGATKAFYVPGCASMPPELAERLR
                     GAPLVFFDGTLWTDDEMIRGHVGVKTGQRMGHMSVSGADGTIAAFAGLDVTRKVFIHI
                     NTTNPILLDDSAERAKAEAAGWEVSYDGMEVSA"
     gene            5131..5931
                     /gene="pqqC"
                     /locus_tag="VGB88_02305"
     CDS             5131..5931
                     /gene="pqqC"
                     /locus_tag="VGB88_02305"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX9646301.1"
                     /translation="MSDAALARVKPTQWYGIEVDEDRLLTPDEFSEAMQQIGRERYHN
                     LHPFHKLLHGGALDHGQVQAWALNRYYYQASIPRKDLTLMARLPSAELRRAWRSRVLD
                     HDGVDVETGGIARYLQLTERLGLDRDYVTSTRGILPGTRFAVDAYVHFVAERSELEAI
                     ASSLTELFAPSIHKERISGLSQHYDWADDYALAYFKKRLGEAPRDALFGRQYVIDHAR
                     TRAEQEAVLNAIRFKTDVLWAQLDALYLAYVEPGMIPPGAFVPGAAGR"
     gene            5928..6215
                     /gene="pqqD"
                     /locus_tag="VGB88_02310"
     CDS             5928..6215
                     /gene="pqqD"
                     /locus_tag="VGB88_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX9646302.1"
                     /translation="MIERLIIDGAARPKLPRHAKLRFDKPRDKWLILAPERVFELDQI
                     AYEIVSLCDGDRALSDVVDTLAAKFEAPREVIDKDVTDLLQGLADKGFIVS"
     gene            6212..7348
                     /gene="pqqE"
                     /locus_tag="VGB88_02315"
     CDS             6212..7348
                     /gene="pqqE"
                     /locus_tag="VGB88_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX9646303.1"
                     /translation="MTEVIAHAHGAEAKAPDRLVPYAVLAELTHRCPLQCPYCSNPLE
                     LERASGELSTAEWRRVMDEAVDMGVLHVHFSGGEPTVRKDLEDLVAHAAKVGLYSNLI
                     TSAVLLSEDRVTRLADLGLDHVQISFQDSTPELGDRIAGYKGAIDKKTQAARWVRKAG
                     IPLTVNAVMHRQNLHHLEDMIEMAVALDAERIEVAQVQYYAWAFRNRAAFLPTRAQLD
                     RAVEIVAEARERLKGVLVFDFVVPDYYAQRPKSCMGGWGRQFINISPSGKVLPCHAAE
                     IIPDLHFETVRDKPLEWIWEHSEAFNRFRGTAWMPEPCRSCDRREIDWGGCRCQALML
                     TGRADVTDPACALSPDRALLEEPLKEALGPPPPFVYRRYGNADA"
     gene            7366..8214
                     /locus_tag="VGB88_02320"
     CDS             7366..8214
                     /locus_tag="VGB88_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9646304.1"
                     /translation="MPVVDLPPLDKAEVARNWAGRAPAWNRWAEQIEKLAERFNRPLI
                     EAARIAPGMTVLDLASGTGEPALSIARLVGPDGRVVATDLVDDMLAGTRRRAEAAGLA
                     NLDCRIADMEALGFADASFDRVTCRFGIMFVPDPVTAMAEARRVLKPGALSAWMVWGP
                     MPDTTLFEVIQREVRAELGLAPEPDLPQFRYARAGLLAEVMRAGGLAEVAEAELRFTA
                     SPPAGSRFWEANVEMSFGEQLAGLTDAQRSRLDARVERAFDAYLDGDRYRIEVHVRIG
                     TGVRPG"
     gene            complement(8216..10084)
                     /locus_tag="VGB88_02325"
     CDS             complement(8216..10084)
                     /locus_tag="VGB88_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX9646305.1"
                     /translation="MPTHRDHPPRRQRDFRTIATLLPYLWPPRRADLKLRVVVAMAFL
                     IGAKVAVVQVPLLLGQVVDALDPAGAAALAAVPVALVLAFGVARLASVAFAELRDAVF
                     ARVAQQAVREVALRTFRKLHALSLRFHLDRRTGGLSRIIERGTSGIEFLLTFMLFNVL
                     PTLLEIGLVVGILLWRYEAPFAAITLASVVFYIGFTLWVTEWRTRFIRRMNDADEQAN
                     TKAIDSLLNYETVKYFGNEEHEAQRFDRSLQRYQNAAVTTKVSLSLLNIGQGFVVSVG
                     MIAALLLAARGVAAGGLKLGDLVAINAFMLQLYQPLGFLGFVYRQIKQSLVDMERMFD
                     LLDEAAEIEDRPDAVALHVTGGVVEFDRVSFGYTPARPILHDLSFTVPARRTVAIVGP
                     SGAGKSTISRLLFRFYEVGGGAIRIDGQDLREVTQDSLRAAIGIVPQDTVLFNDTIRY
                     NIAYARPGCSDAEVEEAARLAQIDEFIASLPDGYDSMVGERGLKLSGGEKQRVAIARA
                     LLKRPKIMLFDEATSALDSATEKEIQANLAELSRGYTTLVIAHRLSTVVDADEILVLD
                     RGRLVERGRHLDLLTKGGLYAEMWRRQRETRRVAERLAELEEVAGAPATTAVPAGE"
     gene            complement(10144..11715)
                     /locus_tag="VGB88_02330"
     CDS             complement(10144..11715)
                     /locus_tag="VGB88_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9646306.1"
                     /translation="MRRLVTLSAATVLLVAIAVLGYVWWSRGDDDVDGVDVVTAAGAP
                     VDVPEAVAESEPPAQPEVDRSVPAPPPSELVSPGLLLEDAPPRLTGPPARLAVSEQAA
                     GAGASLAAPAATARPSDAGPPEQALVPPPAPRAAAREAMPAAPPEVPETAAGPGSSGP
                     GIQAPVTIPAPPAPAAGEALPGGALAVPETAAGPGSAGPGGQRLAAAVPAPAPAREEA
                     AASAPLAAPESRPQAVSNPPGVRAPSFDIVRISPQGGAVIAGRAEPGAEVAVVEHEQL
                     IGAAVADARGEWVVLAERPIAPGTRELGIVARSPSGLEARSESVVVLVVPEPESTPGA
                     PAAPAEAGAEPLAVLMPRGDEGVVRLLQGGEPSAGLEAPGGLRLESLTYDDAGDFDIA
                     GQALPGSDIAAYLDDRLIGIASAGAEGRWLLSPGHTVAPGLYTLRIDQQGEGGAVVAR
                     IETPVSIADLKDWAVAEGLVVVQPGNSLWRIARRVYGRGVRHTLIFEANRDQIRDPDL
                     IYPGQIFLVPGAAEG"
     gene            complement(11820..12404)
                     /locus_tag="VGB88_02335"
     CDS             complement(11820..12404)
                     /locus_tag="VGB88_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9646307.1"
                     /translation="MTRTRLLTASLAAAAVLAAPAALAEPATYAVDPAHTGLYFRIDH
                     VGYSRVFGRFDSMTGSILFDPDAPAAGRIELAIDPASVDTNHAARDDHLRSPDFFNAA
                     EFPELTFRSTAIEVTGDNRATVTGDFTMLGVTMPVSLDVTFNRLAPYPFAEGQVRVGF
                     SATGTVRRSDHGMSYALDALGDEVELLLEVEAFR"
     gene            complement(12439..13308)
                     /locus_tag="VGB88_02340"
     CDS             complement(12439..13308)
                     /locus_tag="VGB88_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646308.1"
                     /translation="MEAWLADPRIQSTVVPFAVAAAVAGVARAAAGTRAEAWAALAVI
                     AGFAAAYALILGAPPLPPRSSAHKIAYLGAAAAALGLGLDLARARPPLVRLVAAGGAG
                     AAALWLLGARLDLDDPATLLWPALPLVAAWLVVMLRLDSQAGAGLTAAVVIMVAAAAL
                     ALVALLGRSASISQLAAALAAAMAGLALWNWPVARIAGGRAATFAGGALLLALASQAL
                     LFTEAAAAALAVLLLVPFADRPAGWLLARLVAPGGRIGQALAPVVLALAAAVPAAVAV
                     AIAHIAAGPLRPF"
     gene            13470..14036
                     /locus_tag="VGB88_02345"
     CDS             13470..14036
                     /locus_tag="VGB88_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9646309.1"
                     /translation="MCVYCGSQTGVEPAHAALARGLGRALAARDIELVFGGGAVGLMG
                     VLADAALGAGGRVTGVIPHFLEQPEIAHPGVTRMIVVDSMHARKQRMFEAADAFAALP
                     GGLGTLDETVEMITWRLLGLHDKPVVLIDHRGYWRPLLALVEHMIAAGFARASVRDLC
                     TIVESVDGLFDALAARPAPHLAADSERL"
     gene            14109..15332
                     /locus_tag="VGB88_02350"
     CDS             14109..15332
                     /locus_tag="VGB88_02350"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9646310.1"
                     /translation="MPKITVANPVVELDGDEMTRIMWRFIKDKLILPYLDVELVYYDL
                     GIESRDATDDAITVAAAEAIKRHRVGVKCATITPDEARVREFNLKRMYRSPNGTIRNI
                     LGGTVFRQPIICRNVPRLVPGWTKPIVIGRHAYGDQYRATDFLVPGKGRLTMRFEPAG
                     GGEAIEREIFDFPGSGIALGMYNLDDSIQGFARACLNYALGLGWPLYMSTKNTILKAY
                     DGRFKDLFQELFEAEFADRYAAAGITYEHRLIDDMVACALKWEGGFVWACKNYDGDVQ
                     SDIVAQGFGSLGLMTSVLLTPDGEVVEAEAAHGTVTRHYRQYQQGKETSTNPIASIFA
                     WTRALWYRGKFDDTPEVQRFAEALERVCVDTVEAGSMTKDLAILIGPDQGWLNTTQFL
                     DALDRRLKAEMAKAA"
     gene            complement(15304..>15740)
                     /locus_tag="VGB88_02355"
     CDS             complement(15304..>15740)
                     /locus_tag="VGB88_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9646311.1"
                     /translation="SAAYALSAFPAGLIADRAGRKLPFALGCAALVAADLVLAAAGGV
                     AGALAGVALWGLHMGLTQGVLATLVADTAPAPLRGSAFGLLNLVTGVIALAASVLAGV
                     LWDRIGPPAPFIAGAAFTVIALAGLALVRPAGRLMPPLPSRP"
BASE COUNT         2301 a         5714 c         5587 g         2138 t
ORIGIN      
        1 gacgtgatcc tcgacggcgg ggtgcggcgc ggcaccgacg tgctcaaggc ggtggcgctc
       61 ggcgccaagg cgtgcatggc cggcaggccc tatctgtacg gcctcgcggc cggcggcgag
      121 gcgggcgtcg accgcgccct cggcctgctc aaggacgaga tccgccgcga catggcgctg
      181 ctcggggtgc gcgacctcgg ccagctcggc cccgcccgca tccgagcgcg ccccgaggca
      241 ctctcgcatc cccccgcgcg cgcccaagcc gcgccgccgc cgcgcctcga cgtggtcaag
      301 tcaggctagg agccggcaag acgactctgc gacaggggaa cagcgtcgtc gcgaggcggc
      361 aatgccgccg acgcgatcca gtccgatctt gctaacagcc cctggattgc tgcgcggcgc
      421 tcgcaatgac gagctgggct cgggttgacc cgttcgtgaa caactctcga cgtcggtatc
      481 ctgatagttg gcatggcatc ggtttgaacg cgaacgatgc ctagcccgcg gcaccggtcc
      541 agccggctgc ggccagcggg gcgcgcggga tgatcaggcg cgccgccatc accggccgcg
      601 gcgaggtgcc gaccctcaag tcgccgatcg cgccgagcag catgtaggcc tgctcgcggc
      661 cgaggtcggt ggtccgcgcc aggtagtcga cagcgtggcg ggtggcgtcc tcggtcgccg
      721 cctcgaccgt ctcgccgaag ccgacgacct ggatcgagtc ggcgcgctcg atcagcggcc
      781 gggcgacggc gcgccccttg tcgagctcga tgctcgcctc gagggtgacc gcgcactcga
      841 ttccggtgcc gctgacgatg ccgtccttga tcgcggcgtg cgggtcggcg aagaacacgc
      901 ccgcgccggg catcagcacc ggcaagtgca ggcgcgaccc ggcctcgacg tggcgctggt
      961 cgaggtcgcc gccataggcg cccgcatagg ccggcttgta gcccgcgggc ggcatggtcg
     1021 agacggtgcc gagattgggc tcgatcgcga ccgggatgcg gtcgccgaac caggccatgc
     1081 cgtcgcggat cgggcaggcg atgatgcgcg ggtcggggaa ctcctcgcgc agcaccccga
     1141 aggcgcgcag gatcgcgttg cgccccaccc cgtggggggt gatgcgcctg agctcgatca
     1201 ccaccgcatc gcccggctcg gcgccgcgga cctcgatcgg gccggtgatc ggcgccagcg
     1261 ggtggccctc cgattcggag gtgaacgggg tcggcacggc ggtgatgtcg tcgatgtcgt
     1321 cgaacgcgcc ctcgacctcg accgtgaact ccgcgcccgg atcgacggtc atcagcggcg
     1381 gggtgtcggg ccccaccgag aacagataga ccttgctcat cgctttcctc ccgcgccaag
     1441 acgccttggc atcctatatt gtttcgatgg acggcgggtc tgccgacagc ccgcaaccca
     1501 ccgccgcccg ggcggcatgg tttgcgcgca atccgggcat ggggtagtct cgccggccac
     1561 ttggagcgct gcggggactc tgcgcaaagg gggacagatg gtctcgaacc agcgacaggc
     1621 ccggacgggc tggcggctcg ccgcattcgg cgcgctcgcc ttcgccctcg ccggctgcct
     1681 gatgtcacgc gcctacgacc agccgatggg ccaggacctg acggtggcga agcgcaacgt
     1741 catgatttcc ggatacgaga accgggtcga ggtcgaaggc gagaccttcg tcttcgaggg
     1801 cccgttggcg catgagcagc acgcctacgc ggtccgctac atggggctcg attcgcgcgg
     1861 caatccggtg ctgcaggtga tcgccactcc gctcgcgcgc accgacgtgc cggtcgagct
     1921 ggccgagacc gaccgcatgc acctgagcgg ctacggcatc ccgccgatca ccgtgcaggt
     1981 ggtcaacgcc accgacgagc tgctcaccta ccggctgcgc gcagacgggc cgttcaaggc
     2041 cacggttcag tagccggcgc cggtcgccgc gccgcacgct tgccgccggg gcatcggcat
     2101 ggtccagaac tcgacccacg gcacgctcga ggtgatcagc ttcgggctgg ccggcggggt
     2161 ctgcgccgcc ctgatcaccc tggtgctcgg gttcagcgcg atggtgttcg gctggggggt
     2221 ggccgcggtc caggtgctgt cctcgctgct ggttggattc ggcccgagct tcgtcgggct
     2281 cgccgcgggc gtggtgtggg cgttcttcga cggcttcgtc gcctccctgc tgctggcctg
     2341 gctgtacaac cgcttcgtgc tcaggcgccg gcggcacatc atctgaggct ggcgatcctc
     2401 tagcccgcgt cctcggcggc gagccgcgcc tcgatctcgt ggcgctggat cttgccggtg
     2461 gtcgagcgcg gcagctcgtc gagcgcgacg aagcggatct ccttcggccg cttgtagccg
     2521 gccaggcgct cgcggcagcg cgccatcagc tcgtcggcgc cgagcgagtc gtcgcggcgc
     2581 gcgaccaccg ccaccggcac ctcgccccag cgcgcgtcgg cgcggcgcac caccaccgcg
     2641 tcggcgaccc tcgggtcggc cagcagcacc tgctcgatct cggccggata gacattctcg
     2701 ccgccggtct tgatcaggta cttggcgcgg tcgacgaagt cgaggctgcc gtcggcgttg
     2761 cgcacgaaca catcccccat gtggaaccag ccgccgcgga agtcgcgggc gttggcctcg
     2821 ggcgcgttcc agtagcccga gaacagggtc gggccgcgga tcgcgacctc gcccggggtg
     2881 ccgtcgggga catcgttgtc cgcctcgtcg accaggcgca gctcgcacat cgagctctgc
     2941 cgcttcgaca gccgctcggg aacctcgccg ggcgggatca gcgcgcccga ggcgggcggg
     3001 atgccggtct cggtggcgcc gaagctgttg agatagggcg ctgcgagcgc gcgcgtgacc
     3061 tcggcgatca cgtggcgcgg caccaggtcg gccatcgccc cggtcatgcg cacgcccttg
     3121 accgcgagcg gcgcggcccg gtggcgctca agcagccggt cgatcacccc cggcatgagg
     3181 atcaaccagc cgaccgggtg gccggccagc acctcggcga tgtggtcgag gtcgaagccg
     3241 tcgatcagcg ccacccggcc gccgatcaga agcgtgccga ttgcgaggtc ggtggacgcc
     3301 atgtggaaca tcggcgccca ggccgggaac agctcgtcgc ggccaatccc gtagtcggcg
     3361 cagaacacca gcacccggct gatgaacgca cgctggctga ccaccgcgcc cttgggccgg
     3421 ccggtggtgc cgctggtgta gaggatcacc aggccgtcct cggggtcggc gacctcgacg
     3481 ggctcggtct cgggcgcggc ggcgagcatc gcctcgtaag gctcgcccag ctcgaccacc
     3541 gtcgccgggc gcgcatcgag cgcggccagc gccgccgcgt ggcgcggcga gaccagcgcc
     3601 gccttgggcg tggtcagggc gacgcagtag tcgagctcgt ccgcgccgag ccgccagttg
     3661 agcgcgggca cgatcgcgcc gagcttggcg gcggcgaact cgagttccag gtattcggcg
     3721 cggttctcgg acaggatcgc gacccggtcg ccgcggccga tgccgcgcgc ggcgagcgcg
     3781 tgcgccaggc ggttgacccg ccggttgagc tcccgatagg tccacgaccg ccggcggtcc
     3841 tcgaccgcga tccggtcggg ctggctttgc gcccggtcgc gcagcagccc gccgacggtt
     3901 gcccggcctg cggcggccgc gatggcggcg ctattgggaa tcatgatcgc tgctataggt
     3961 ttttttgaat ggttcaaaac tctatcttcc gccggtcgct tccggagcat ccggaggaga
     4021 cagtacagaa accatccaca atgtggagga tgacatcatg tggagtaccc ctgtcgttcg
     4081 tgagatcgcc gtcggtctcg agatcaactg ctacgcctgc gccgacctct aaggcacagg
     4141 ccacgagctg gaatggtaaa cctctccgtc ggcggagacg gggccgtgac cactcggagg
     4201 cgatgtgatg cgaatccgcg tgttgggctc agccgccggc ggagggtttc ctcagtggaa
     4261 ctgtaatcat cccaacagcc gccgcgcccg caccggcgat ccggcggcca agcgacgcac
     4321 ccagtcctcg atcgcgatca gcgccgacgg ccggcgctgg ttcctgtgca acgcctcgcc
     4381 cgacctgcgc cagcagatcc tcgacaacga cgtcctccat ccccgcaacg gcctgagaaa
     4441 cagcccgatc cagggcgtgg tgctgaccaa cgccgacgtc gaccatatcg ccgggctgct
     4501 gaccttgcgc gagtcgcagc cgctcgcgat ctatggcacc gcgcgcgtgc tcgcggtgct
     4561 cgccgccaac aacgtgttca acgtgcttaa ccccgagctc gtcgcgcgcc ggccgctcgc
     4621 gctcgacgcg cccaaccggc tggtctgccc ggacggcggc gacagcggcc tcgaggtggt
     4681 gccgttcccg gtgccgggca aggtggcact ctggctcgag gacgcgtcca agggcgccaa
     4741 cttcggcacc gtcgcggagg acactgtggc gctcgagatc cgcgaggacg gcgcgaccaa
     4801 ggcgttctac gtgcccggct gcgcgtcgat gccgccggag cttgccgagc gcctgcgcgg
     4861 cgcgccgctg gtgttcttcg acggcacgct ttggaccgac gacgagatga tccgcggcca
     4921 tgtcggcgtc aagaccggcc agcgcatggg ccatatgagc gtctccggcg ccgacggcac
     4981 gatcgccgcc ttcgccggtc tggacgtcac gcgcaaagtg ttcatacata tcaacaccac
     5041 caacccgatc ctgctcgacg acagcgccga gcgtgccaag gccgaggcgg cgggctggga
     5101 ggtgtcatat gacggcatgg aggtatcggc atgagcgacg cagccctcgc ccgcgtcaag
     5161 ccgacgcaat ggtacggcat cgaggtcgat gaagaccgcc tgctcacgcc cgacgagttc
     5221 agcgaggcga tgcagcagat cgggcgcgag cgctaccaca acctgcaccc gttccataag
     5281 ctgctgcacg ggggtgcgct cgaccacggc caggtccagg cctgggcgct caatcgctac
     5341 tactaccagg ccagcatccc gcgcaaggac ctgaccctga tggcgcgcct gcccagcgcc
     5401 gagctcaggc gcgcatggcg cagccgggtg ctcgaccacg acggcgtcga cgtcgagacc
     5461 ggcggcatcg cgcgctatct gcagctcacc gagcggctcg ggctcgaccg cgactacgtc
     5521 acatcgaccc gcggcatcct gcccggcacg cggtttgcgg tcgatgccta tgtccacttc
     5581 gtcgccgagc gctcggagct cgaggcgatc gcctcgtcct tgaccgagct gttcgcgccg
     5641 tcaatccata aggagcgcat ctcggggctg tcgcagcatt acgactgggc cgacgactac
     5701 gcgctcgcct acttcaagaa gcgcctcggc gaggcgccgc gcgacgcgct gttcggccgc
     5761 cagtacgtga tcgaccacgc tcgcacccgc gccgagcagg aagcggtgct gaacgcgatc
     5821 cggttcaaga ccgacgtgct gtgggcgcag ctcgacgcgc tgtatctggc ctatgtcgag
     5881 ccgggcatga tcccgccggg cgcgttcgtg cccggcgccg cggggcgatg atcgagcggc
     5941 tgatcatcga cggcgcagcg cggcccaagc tgccgcgcca cgccaagctg cgcttcgaca
     6001 agccgcgcga caaatggctg atcctggcgc ccgaacgggt gttcgagctc gaccagatcg
     6061 cctacgagat cgtctcgctg tgcgacggcg accgcgcgct cagcgacgtc gtcgatacgt
     6121 tggcggcgaa attcgaggcc ccgcgcgagg tgatcgacaa ggacgtcacc gacctgctcc
     6181 agggcctggc cgacaagggg ttcatcgtgt catgacagag gtaatcgcgc acgcccacgg
     6241 cgccgaggcg aaggcgccgg accggctggt gccctatgcg gtgctggcgg agctgaccca
     6301 ccgctgcccg ctgcagtgcc cgtactgctc gaacccgctc gagctcgagc gggcgagcgg
     6361 cgagctcagc accgcggagt ggcggcgggt gatggacgag gcggtcgaca tgggcgtgct
     6421 ccacgtccac ttctcgggcg gcgagccgac cgtgcgcaag gacttggaag acctggtcgc
     6481 gcacgccgcc aaggtcggcc tgtacagcaa cctgatcacc tcggcggtgc tgctgagcga
     6541 ggaccgggtc acgcgcctcg ccgacctcgg cctcgaccac gtccagatca gcttccagga
     6601 ctcgacgccc gagctcggcg accggatcgc cggctacaag ggcgccatcg acaagaagac
     6661 ccaggccgcg cgctgggtgc gcaaggccgg catcccgctg accgtgaacg cggtcatgca
     6721 ccgccaaaac ctgcaccacc tcgaggacat gatcgagatg gcggtcgcgc tcgacgccga
     6781 gcggatcgag gtcgcccagg tccagtacta tgcctgggcg ttcaggaacc gcgccgcgtt
     6841 cctgcccacc cgcgcccagc tcgaccgcgc ggtcgagatc gtcgccgagg cgcgcgagcg
     6901 gctgaagggc gtgctcgtct tcgacttcgt cgtgcccgac tactacgccc agcggccgaa
     6961 gtcgtgcatg ggcggctggg gccgccagtt catcaacatc tcgccgtccg gcaaggtgct
     7021 gccgtgccac gccgccgaga tcatcccgga cctccacttc gagaccgtgc gcgacaagcc
     7081 gctcgagtgg atctgggaac actctgaggc gttcaaccgc ttccgcggca ccgcctggat
     7141 gcccgagccg tgccgcagct gcgaccggcg cgagatcgac tggggcggct gccgctgcca
     7201 ggcgctgatg ctcaccggcc gcgccgacgt caccgatccg gcctgcgcgc tgtcgcccga
     7261 ccgcgcgctg ctcgaggagc cgctcaagga ggcgctcggc ccgccgccgc cgttcgtcta
     7321 ccgccgctac ggcaacgccg acgcctgagc ccgcgcgggg gactgatgcc ggtcgtcgac
     7381 ctgccgccgc tcgacaaggc cgaggtcgcg cgcaactggg cggggcgcgc gccggcctgg
     7441 aaccgctggg cggagcagat cgagaagctg gccgagcgct tcaaccggcc gctgatcgag
     7501 gccgcgcgga tcgcccccgg catgaccgtg ctcgatctcg cctcgggcac cggcgagccg
     7561 gcgttgagca tcgcgcgcct ggtcgggccc gatgggcggg tggtggcgac cgacctggtc
     7621 gacgacatgc tcgccggcac ccggcgccgc gccgaggccg cgggcctcgc caacctcgac
     7681 tgccggatcg ccgacatgga ggcgctcggc ttcgcggacg cgagcttcga ccgggtcacc
     7741 tgccggttcg ggatcatgtt cgtgcccgac ccggtcacgg cgatggccga ggcgcggcgc
     7801 gtgctcaagc cgggcgcgtt gagcgcctgg atggtgtggg ggccgatgcc cgacaccacc
     7861 ctgttcgagg tcatccagcg cgaggtgcgc gccgagctcg gcctcgcgcc cgagcccgac
     7921 ctgccccagt tccgttacgc ccgcgccggc ctgctggcgg aggtgatgcg cgccggcggc
     7981 ctggccgagg tcgccgaggc cgagctcagg ttcaccgcca gcccgccggc cggctcgcgc
     8041 ttctgggagg ccaatgtcga gatgtcgttc ggcgagcagc tcgccggcct gacggacgcg
     8101 caacgcagcc ggctcgacgc gcgtgtcgaa cgcgccttcg acgcctatct cgacggcgac
     8161 cgctaccgca tcgaggtcca cgtccgcatc ggcaccgggg tacgcccggg ctgagctact
     8221 caccggcggg cactgccgtc gtcgcgggcg cgcctgcgac ctcctcaagc tcggcgagcc
     8281 gctcggcgac ccggcgcgtc tcgcgctggc gccgccacat ctcggcatag agcccgccct
     8341 tggtgagcag gtccaggtgg cggccgcgct cgaccagccg gccgcggtcg agcaccaata
     8401 tctcgtcggc gtcgaccacg gtcgacaggc gatgggcgat gacgagggtg gtgtagccgc
     8461 gcgacagctc ggcgaggttc gcctggatct ccttctcggt ggccgaatcc agcgccgagg
     8521 tggcctcgtc gaacagcatg atcttcggcc gcttgagcag cgcgcgcgcg atcgccaccc
     8581 gctgcttctc gccgcccgac agtttcagcc cgcgctcgcc gaccatgctg tcgtagccgt
     8641 cgggcaggct ggcgatgaac tcgtcgatct gggcgaggcg cgccgcctcc tcgacctcgg
     8701 cgtccgagca tccgggccgc gcataggcga tgttgtagcg gatggtgtcg ttgaacagca
     8761 ccgtgtcctg ggggacgatg ccgatcgcgg cgcgcagcga gtcctgggtg acctcgcgca
     8821 ggtcctggcc gtcgatccgg atcgcgccgc cgccgacctc gtagaaccgg aacaagagcc
     8881 gcgagatggt agacttgccg gcaccgctcg ggccgacgat cgccaccgtc cgccgcgcgg
     8941 gcacggtgaa gctgagatcg tggaggatcg ggcgggcggg cgtgtagccg aacgagacgc
     9001 ggtcgaactc gaccaccccg ccggtgacgt gcaacgccac cgcgtccggc cggtcctcga
     9061 tctcggcggc ctcgtccagc agatcgaaca tgcgctccat gtcgaccagc gactgcttga
     9121 tctggcggta gacgaagccg aggaagccga gcggctggta gagctggagc atgaacgcgt
     9181 tgatcgccac caggtcgccg agcttgaggc cgcccgcggc caccccgcgc gcggccagca
     9241 gcagcgccgc gatcatgccg acggaaacca cgaacccctg gccgatgttg agcagcgaca
     9301 gcgacacctt ggtcgtgacc gcggcgttct ggtagcgctg cagggacctg tcgaagcgct
     9361 gggcctcgtg ctcctcgttg ccgaaatact tcaccgtctc atagttgagc aggctatcga
     9421 tcgccttggt gttggcctgc tcgtcggcgt cgttcatgcg ccggatgaag cgggtccgcc
     9481 attcggtgac ccacagcgtg aagccgatat agaacaccac cgaggcgagc gtgatcgccg
     9541 cgaacggcgc ctcgtagcgc cagagcagga tgccgaccac caggccgatc tcgagcaggg
     9601 tcggcagcac gttgaacagc atgaaggtga gcaggaactc gatgccgctg gtgccgcgct
     9661 cgatgatccg gctcaagccc ccggtgcgcc ggtcgagatg gaagcggagc gacagcgcgt
     9721 gcagcttgcg gaaggtgcgc agcgccacct cgcgcaccgc ctgctgggcg acgcgcgcga
     9781 acaccgcgtc gcgcagctcc gcgaacgcga ccgaggcgag ccgggcgacg ccgaaggcga
     9841 ggaccagcgc gaccggcacc gcggcgagcg cggcggcgcc ggccggatcg agcgcgtcga
     9901 ctacctggcc gagcaacagc ggcacctgga ccaccgccac cttggcgccg atcaggaacg
     9961 ccatcgccac caccacccgc agcttcaggt ccgcgcgccg cggcggccac agatagggca
    10021 gcagcgtggc gatggtcctg aagtcgcgct gtcggcgcgg cgggtggtcg cggtgtgtcg
    10081 gcattccggt cgctgcggtc atggctcgtt accgccgacc gtgcccggtc ggcgccgcgc
    10141 cggtcagccc tccgccgcgc cgggcacgag gaagatctgg ccgggataga tcaggtcggg
    10201 atcgcggatc tgatcgcgat tggcctcgaa gattagcgtg tgccgcacgc cgcggccata
    10261 gacccggcgc gcgatccgcc acaggctgtt gcccggctgg accaccacca gcccctcggc
    10321 caccgcccag tccttgaggt cggcgatcga gaccggggtc tcgatccgcg ccaccaccgc
    10381 cccgccctcg ccctgctggt cgatcctgag cgtgtacagg cccggcgcga cggtgtggcc
    10441 cgggctcagc agccagcgcc cttcggcgcc ggcgctggcg atgccgatca ggcgatcgtc
    10501 gagataggcg gcgatgtcgc tgccgggcag cgcctggccg gcgatgtcga agtcgccggc
    10561 atcgtcgtag gtcagcgact cgagacggag gccgccgggc gcctcgagcc ccgccgacgg
    10621 ctcgccgccc tggagcaggc gtaccacgcc ctcgtcgccg cgcggcatca gcaccgccag
    10681 cggctcggcg ccggcctcgg ccggcgccgc aggggcgccc ggcgtcgact cgggctcggg
    10741 caccaccagc accaccacgc tttccgagcg cgcctcgagg ccggatggcg agcgcgcgac
    10801 aatgccgagc tcgcgcgtgc ccggcgcgat cggccgctcg gccagcacca cccactcgcc
    10861 gcgggcgtcg gcgacggcgg cgccgatcag ctgctcgtgc tcgaccacgg cgacctcggc
    10921 gccgggctcg gcgcgtccgg cgatcacggc gccgccttgc ggcgatatgc gcacgatgtc
    10981 gaagctcggc gcgcgcacgc cgggcgggtt gctcaccgcc tggggccgcg attcgggtgc
    11041 cgcgagcgga gcgctcgccg cggcttcctc gcgggcaggc gcgggcgcgg gcaccgccgc
    11101 agcgagcctc tggccgccgg ggcctgcact gcccggcccg gcggcagtct ccggcaccgc
    11161 cagcgccccg cccggcagtg cctcgcccgc ggcgggcgca ggcggcgccg ggatggtgac
    11221 cggcgcttga attccaggcc cggagctgcc cgggccggcg gcggtctccg gcacctccgg
    11281 cggcgcggcc ggcatggcct ctcgggcggc agccctgggc gccggcggcg gcaccagcgc
    11341 ctgctccggc ggccccgcgt ccgacggccg tgcggttgcc gccggcgccg ccagcgatgc
    11401 gcccgcgccc gcggcctgct cgctgaccgc aagtcgcgcc ggcggcccgg tcagtcgcgg
    11461 cggcgcgtcc tccagcagca gcccgggcga gaccagctcc gagggcggcg gcgccggcac
    11521 gctgcggtcg acctccggct gtgccggcgg ctccgattcg gcgaccgcct ccggcacgtc
    11581 caccggcgcg cccgcggcgg tgaccacgtc gactccgtcc acgtcgtcat cgccgcggct
    11641 ccaccagacg tagcccagca ccgcgatggc caccagcagc accgtcgccg cgctcaacgt
    11701 caccagccgc cgcacggccc aactccttca gcccgttggc ttttcggcgg aacagtaata
    11761 ccagcgacac ggaaccgcaa tcggcgcgtg ggcaaccggc gacaatcggc cgcggcggct
    11821 cagcggaacg cctcgacctc gagcaacagc tcgacctcgt cgccgagcgc gtcgagcgca
    11881 taggacatgc cgtggtcgga gcggcgcacc gtgccggtgg ccgagaagcc gacccgcacc
    11941 tgaccttccg cgaacgggta cggcgccagg cggttgaagg tcacgtccag cgacaccggc
    12001 atggtgacgc cgagcatggt gaagtcgccg gtcacggtcg cccggttgtc gccggtcacc
    12061 tcgatcgcgg tgctgcggaa ggtcagctcg gggaactcgg cggcgttgaa gaagtcgggc
    12121 gagcgcaggt ggtcgtcgcg cgcggcgtgg ttggtgtcga cgctcgccgg gtcgatggcg
    12181 agctcgatac ggcccgccgc gggcgcgtcg ggatcgaaca ggatcgagcc cgtcatggaa
    12241 tcgaagcgcc cgaacacgcg cgaatagccg acatggtcga tgcggaagta gaggccggtg
    12301 tgcgcgggat cgacggcgta ggtcgccggc tccgccagtg cggccggcgc cgccagcacg
    12361 gcggcggcag ccagcgatgc ggtcagcagt cgggtccggg tcatgggtcc tcccctcggt
    12421 tggttcgagt tcgatcggtc aaaacggcct gagcggcccg gcggcgatgt gggcgatcgc
    12481 caccgcgacc gcggcgggca cggcggcggc cagcgccagc accaccggcg cgagcgcctg
    12541 gccgatgcgc ccgcccggcg ccaccaggcg ggcgagcagc cagccggccg ggcggtcggc
    12601 gaacggcacc agcagcagca ccgccagcgc cgccgccgcg gcctcggtga acagcaacgc
    12661 ctggctggcg agcgcgagca gcagagcgcc gccggcgaag gtcgcggcgc gcccgccggc
    12721 gatgcgcgcc accggccagt tccacagcgc gaggccggcc atcgccgccg ccagcgccgc
    12781 ggcgagctgg gaaatcgagg cgctgcgccc gagcagcgcg accagcgcca gcgccgccgc
    12841 ggcgaccatg atcaccaccg ccgcggtcag gccggcgccg gcctgggagt cgagcctgag
    12901 catcaccacc agccaggccg cgaccagcgg caatgccggc cacagcaggg tcgccgggtc
    12961 gtcgaggtcg aggcgcgcgc cgagcagcca cagcgccgcc gcgcccgcgc cgccggccgc
    13021 gaccaggcgg accagcggcg gccgggcgcg ggcgaggtcg agcccgagcc cgagcgccgc
    13081 cgccgccgca cccagatagg cgatcttgtg cgcgctcgac cgcggcggca gcggcggcgc
    13141 gccgaggatc agcgcatagg ccgcggcgaa gcccgcgatc acggcgagcg cggcccacgc
    13201 ctcggcgcgc gtgccggccg ccgcgcgcgc cacgccggcg accgccgccg cgaccgcgaa
    13261 cggcaccacc gtgctctgga tcctgggatc ggcgagccac gcctccaccg ccggcctccg
    13321 catgcgcagt tgccggcgca tgtaccacgg cgcgaccggc gcgtcatccg gccgcggccg
    13381 cggccacggg cttgtgatcg gccggcccgc ctttcgtgct accgtgtgcc ggcaaccggc
    13441 gagggagagc ggtggccgag attcgtgcaa tgtgcgtcta ttgcggctcg cagaccggcg
    13501 tcgagccggc gcatgccgcg ctcgcgcgcg ggctcgggcg cgcgttggcc gcgcgcgaca
    13561 tcgagctcgt gttcggcggc ggcgcggtcg ggctgatggg cgtgctcgcc gacgccgcgc
    13621 tcggcgccgg cggccgggtc accggggtga tcccgcattt cctcgagcag cccgagatcg
    13681 cgcatccggg cgtcacccgg atgatcgtgg tcgacagcat gcacgcccgc aagcagcgca
    13741 tgttcgaggc ggcggacgcc ttcgccgcgc ttccgggagg tctcggcacg ctcgacgaga
    13801 cggtcgagat gatcacctgg cggctgctcg gcctgcacga caagccggtg gtgctgatcg
    13861 accatcgcgg ctactggcgg ccgctgctgg cgctggtcga gcacatgatc gcggccggct
    13921 tcgcgcgcgc ctcggtgcgc gatctctgca cgatcgtcga gtcggtggac gggctgttcg
    13981 atgcgctcgc cgcccggccg gcgccgcacc ttgccgccga cagcgagcgc ctgtagcggc
    14041 ctttgcaagg ccggggccgc ttgttatacc ccgtccgcgc caccgacgcc gccgaggagc
    14101 ttccggccat gcccaagatc acggtcgcca acccggtcgt cgagctcgac ggcgacgaga
    14161 tgacgcgcat catgtggcgc ttcatcaagg acaagctgat cctgccctat ctcgacgtcg
    14221 agctggtcta ttacgacctc ggcatcgagt cgcgcgacgc caccgacgat gcgatcacgg
    14281 tcgcggccgc ggaggcgatc aagcgccacc gggtcggcgt caagtgcgcc accatcaccc
    14341 ccgacgaggc gcgggtgagg gagttcaacc tcaagcgcat gtaccgctcc cccaacggca
    14401 ccatccgcaa catcctgggc ggcaccgtgt tccgccagcc gatcatctgc cgcaacgtgc
    14461 cgaggctggt gccgggctgg accaagccga tcgtgatcgg ccgccacgcc tacggcgacc
    14521 agtaccgggc gaccgacttc ctggtccccg gcaagggcag gctgaccatg cgcttcgagc
    14581 ccgccggcgg cggcgaggcg atcgagcgcg agatcttcga cttcccgggc tcaggcatcg
    14641 cgctcggcat gtacaatctc gacgactcga tccagggctt cgcgcgcgcc tgcctcaact
    14701 atgcgctcgg gctcggctgg ccgctctaca tgtcgaccaa gaacaccatc ctcaaggcct
    14761 atgacgggcg cttcaaggac ctgttccagg agctgttcga ggccgagttc gccgaccgct
    14821 acgccgccgc cgggatcacc tacgagcatc gcctgatcga cgacatggtc gcctgtgcgc
    14881 tcaaatggga gggcggcttc gtgtgggcgt gcaagaacta tgacggcgac gtgcagtcgg
    14941 acatcgtggc ccagggcttc ggctcgctcg ggctgatgac ctcggtgctg ctgaccccgg
    15001 acggcgaggt cgtggaggcc gaggccgcgc acggcaccgt cacccgccac taccgccagt
    15061 accagcaggg caaggagacc tcgaccaacc cgatcgcctc gatcttcgcc tggacccggg
    15121 cgctgtggta tcgcggcaag ttcgacgaca cccccgaggt gcagcgcttc gccgaggcgc
    15181 tcgaacgcgt gtgcgtagac acggtcgagg ccggttccat gaccaaggac ctcgcgatcc
    15241 tgatcggccc cgaccagggc tggctcaaca ccacccagtt cctcgatgcg ctcgaccggc
    15301 gcctcaaggc cgagatggca aaggcggcat aagccggccc gcgggtcgaa cgagcgccag
    15361 cccggcaagc gcgatcacgg tgaacgcggc gccggcgatg aacggcgcgg gcgggccgat
    15421 ccggtcccac aggacgccgg caagcacgct cgccgcgagc gcgatcacgc ccgtgaccag
    15481 gttgagcagg ccaaaggccg agccgcgcag cggcgccggc gcggtgtcgg cgaccagggt
    15541 cgcgagcacg ccctgggtga gccccatatg caggccccac aaggcgacgc cggcgagcgc
    15601 gcccgccacc ccgcccgcgg ccgcgagcac caggtcggcc gccaccagcg cggcgcagcc
    15661 gagcgcgaac ggcagcttgc ggccggcgcg gtcggcaatg agcccggcgg ggaacgccga
    15721 cagcgcgtag gccgcgctca
//
LOCUS       DASWHU010000063        14274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_394282, whole genome shotgun sequence.
ACCESSION   DASWHU010000063 DASWHU010000000
VERSION     DASWHU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394282"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /gene="groL"
                     /locus_tag="VGB88_02360"
     CDS             complement(<1..1630)
                     /gene="groL"
                     /locus_tag="VGB88_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382650.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9646312.1"
                     /translation="MAAKDVRFSTDARTRMARGVDILANAVRVTLGPKGRNVLLDKSF
                     GAPRITKDGVTVAKEIELSDKFENMGAQMVREVATKTSDIAGDGTTTATVLAQAIFRE
                     GLKSVAAGMNPMDLRRGVDMAVEAVVQHLTKQAKAIKTSEEIAQVATISANGERAIGD
                     FIARAMDKVGKEGVITVEEAKGLETELEVVEGMQFDRGYLSPYFITNAEKMECELDNP
                     YLLIHEKKLSSLQALLPLLEAVVQSSRPLLVIAEDVEGEALAALVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAVLGGGQVISEDLGIKLESVTLDMLGSAKRVNITKDDTTIVG
                     GAGKRAAIEGRCTQIRQQIDDTTSDYDREKLQERLAKLAGGVAVIKVGGATETEVKER
                     KDRVEDALHSTRAAVEEGIVPGGGVTLIYAARVLDGLNPENEDQKVGVDIVRRALGQP
                     LRQIAENAGVDGSVVVGKVRESKNQRFGFDAQSEVYCDLVQAGIVDPAKVVRIALQDA
                     ASVAGLLITTEAMVAERPEKREAAPMPGGGMGGMG"
     gene            complement(1642..1929)
                     /locus_tag="VGB88_02365"
     CDS             complement(1642..1929)
                     /locus_tag="VGB88_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9646313.1"
                     /translation="MNVRPLQDRVLVRRIEEDERTAGGIIIPDTAKEKPSQGKVLAVG
                     AGARDQNGKLVEPDIKKGDRILFGKWSGSEVKIKGEELLIMKESDIMGVME"
     gene            complement(2054..3343)
                     /gene="hflX"
                     /locus_tag="VGB88_02370"
     CDS             complement(2054..3343)
                     /gene="hflX"
                     /locus_tag="VGB88_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9646314.1"
                     /translation="MASGRARQPGASTRSGRARLDEAVGLARAIDLEVALAEVVPVRT
                     PRPSTLVGRGKVAEIAERLRAADGRPPDVVVVDAALTPVQHRNLEKAWSCKVLDRTAL
                     ILEIFGARARTAEGRLQVELAALTYQRSRLVRSWTHLERQRGALGFIGGPGESQLEAD
                     RRLIDGRIVRIRRALAEVVRTRGLQRAARRRVPYPLVALVGYTNAGKSTLFNRLTGAD
                     VAAADRLFATLDPTMRAAVLPSGRTVILSDTVGFISDLPTDLIAAFRATLEEVILADL
                     IVHVRDAAHADAGAQADDVRAVLRTLDIDDQDRARVVEVHNKIDLLDAGQRQALEARC
                     AGNADAVAVSALAGTGRAALLALIGRKLSHGRRVLDLDVDLGDGAALAWLYRHGEVLT
                     RRDGTAAVHVRVALDPADVARFGDRFGSPQDRPAASR"
     gene            complement(3434..3685)
                     /gene="hfq"
                     /locus_tag="VGB88_02375"
     CDS             complement(3434..3685)
                     /gene="hfq"
                     /locus_tag="VGB88_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX9646315.1"
                     /translation="MAADKTQNVQDVFLNHVRKNKTPLTIFLVNGVKLQGVITWFDNF
                     CVLLRRDGISQLVYKHAISTVMPGTPVQLFEPDKTSEEA"
     gene            complement(3869..4726)
                     /locus_tag="VGB88_02380"
     CDS             complement(3869..4726)
                     /locus_tag="VGB88_02380"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX9646316.1"
                     /translation="MSRIAYVNGRYLPHGGAEMHIEDRATQFSDGVYEVIAVLGGRLI
                     DRALHHERLERSLGEMRIAAPMGRAALDAVLDEVVRRNRVAEGIVYLQIGRGRAPRNH
                     AFPVGVRPSVVVTARRARPATAAALTEGVAVITVPDLRWRRRDIKSVSLLANVLAKQA
                     AAEAGAYEAWQVDDRGLVTEGAQSNAWIVDTDGTILTRPESNDILSGITRRRLIGLAR
                     GSGLAVAERAFTPDHARRAREAFLTSTSSFVVPVIRIDDHPVANGKPGSTTERLRSLY
                     LDFASRTAA"
     gene            complement(4740..6197)
                     /locus_tag="VGB88_02385"
     CDS             complement(4740..6197)
                     /locus_tag="VGB88_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9646317.1"
                     /translation="MAAATILALLGALLVGLAAAELAPLAVALALGEAADVRAFGLGA
                     GTALFFGVVLLLVGRGTWRRVTRRDALVLAALGWAGLSLFAALPFALAGVAPNAPGAV
                     LEAVSGLTTTGLSVIADPAGAGRSVVLWRALLQWIGGAATLVFAATLIPQVGFATRAR
                     RAGASSPERLAEPFRPRFFQAAQAVLVPYAVLTVLCFMALAATGLTSFHAACYALSTV
                     STGGFSPDRGGPLALGNGMAEMVLILFMAAGAINFTVHWAALCGRPRAYGADPETGFM
                     LLVVATAAVLLAVLGVAEGAGAIGAELRRALFQAVSAATTTGYGAGADSGPDPVFVVL
                     VLAGLVLVGGASTSTAGGIKLGRALLLLRQSVRELERLAHPHGVVVIKLGRTSITDSA
                     MEAVWAYFVLFLACLITLALGLAAVGLDAERSMLLALSAIANTGPLLTGAAGLGGSLA
                     SLPETAHWLLALGMLVGRLELFAVLMLLTPEFWQR"
     gene            complement(6228..7604)
                     /gene="trkA"
                     /locus_tag="VGB88_02390"
     CDS             complement(6228..7604)
                     /gene="trkA"
                     /locus_tag="VGB88_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX9646318.1"
                     /translation="MRVLVCGAGEVGFNIARHLAAEDNDVVVIDQAPERIEKVGEALD
                     VQAMLGFASHPDVLKLAGAEDADMLIAVTQSDEINIVACQVAHSLFNVPTKIARIRHQ
                     AYLDPAWADLFSRAHMPIDVVISPEVEVANSIGRRLEVPGALDMTSFADDRVRLVGVR
                     LGESCPVVNTPLGQLNELFPDLNITIVCIVRNEVTLFPERMEQLVAGDEVYFVAETAQ
                     LARAMVLFGHEEQAARRLVIIGGGNIGLLLARQIEKTNPNINLKLIEFDKERAEFVAD
                     RLERTIVINGDALDTDILKEANIANTETVLAVTANDEVNVLASVLAKRHGCARAVTLV
                     NSSTYAPLISPLGIDASVSPRGITVSSILQHIRRGRIRSVYSLRGGEAEVIEAEAMAT
                     SPLVGPPLKDVKLPKGVLVGAIVRGEEVIIPRAANSVKEHDRVIIFAPHDAVKKVERM
                     FAVALGFF"
     gene            complement(7662..9047)
                     /locus_tag="VGB88_02395"
     CDS             complement(7662..9047)
                     /locus_tag="VGB88_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9646319.1"
                     /translation="MTSEVLIVDDERDIRSLIAGILADEGFQTREAWDADSAASEIGA
                     RLPGLVLLDIWLQGSRLDGIALLQQIRARYPELAVVMISGHGNIETAVKAIRLGAYDF
                     IEKPFEADRLLLSVERALEASRLRRENAELRQRAVTESVLIGDSHAMSVLRQAVHKVA
                     PTGSRVLISGPAGSGKEVVARLLHGRSRRTNGPFVVINAAAMTPERMEEELFGVEGAG
                     ENETGPRKIGVFEQAHGGTLLLDEVADMPLQTQGKILRVLQEQTFRRVGGSRNIQVDV
                     RVLASTSKDLPAEITNKKFREDLFYRLNVVPIRVPPLRDRREDIPQLVSHFVSKAGAY
                     GLAGRALAGDAMAVLQTCDWPGNVRQLRNLVDWILIMAPGDIAEPVTADMLPPELLVA
                     TPAAMRSENGAEIMTLPLREAREMFERQYLQAQVTRFGHNISRTASFVGMERSALHRK
                     LKSLGITGDER"
     gene            complement(9044..11314)
                     /locus_tag="VGB88_02400"
     CDS             complement(9044..11314)
                     /locus_tag="VGB88_02400"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9646320.1"
                     /translation="MSIDSSPAAPAGWRRLAPRWPLRPSVTRRLAIAVAIAAGIFGIA
                     TYVLLTGSMSPDGGTVTTVLIMLNIDLVLLLLLGALVAWHLVRLWIERRQGSVGSRLH
                     ARIVAMFSLVAVAPAIVVAVFSATFFNLGIQTWFNDRVRTALDRSLAVSEAYLKDHRE
                     FIRADILSMVATLNRQDSAVLGSPEELNRLLDAMAGERSLSEAIIVRGDGKILARSRL
                     SFVLEFDPIANAALQRAAEGELVIMTSDESDRIRALARIDGMVGAFLYVGRFVDAEVL
                     NHMVRLSSAVSDYKALEARRSGIQITFTLLYVVVALLLLLAAVWIGLVFTNRLIRPIS
                     ALVGATERVRAGDLSVRVAEGHVGDEIAALSRAFNRMTGQLESQRTELIEANTQIDNR
                     RRFTEAVLYGVSAGVLGLDRDGRIFLPNQTALDLLAVSADSLVGQPLERAVPEMADLM
                     ADLRAGSAGHVQDKVSIIRDNRARELLVRIGAQRTHGRITGYVVTFDDITDLVHAQRA
                     AAWADVARRIAHEIKNPLTPIQLSAERLRRKYRARLGEGEQVFSDCVDTIVRQVGAIR
                     GMVDEFSAFARMPAPVLVEADLTGIVAAAVALQRHAHPEIGFEVEAPVPPVALHCDAA
                     QVGQAMTNMLQNAIDAIDGRAANAAALPPGRVAVRIDASSAERLTVAIEDNGRGLPQD
                     LEQRSRLTEPYVTTRERGTGLGLAIVKKIMEDHGGEVQLTDGSMGGARALLVFPRRSS
                     TRASQGPGVDAVEPVR"
     gene            complement(11342..12796)
                     /gene="ntrC"
                     /locus_tag="VGB88_02405"
     CDS             complement(11342..12796)
                     /gene="ntrC"
                     /locus_tag="VGB88_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX9646321.1"
                     /translation="MTEPATILIADDDRSIRTVLGKALGRLGYTVRGAGTAATLWRWV
                     NGGEGDLVITDVVLPDDDGLELLPRLKSVRPDLPVVVMSARNTLLTAIRANERGAYEY
                     LPKPFDLNELIAVVRRALAEPPEGELARPAAAADQPDEGLPLVGRSPAMQEVYRMLAR
                     LMGTDLTVMISGESGTGKELFARALHDYGKRRGMPFVAINMAAIPRELIESELFGHER
                     GAFTGASGRSIGRFEQANGGTLFLDEIGDMPPEAQTRLLRVLQQGEYTTIGGRQPIRT
                     DARIIAATHRDLTQLIERGLFREDLFYRLNVVPLRLPPLRERADDVPILVKHFLARAQ
                     DEGLPAKSVTPEAMARLKSYGWPGNVRELENLVRRLAALYAEEVITEPIVAAELSDGR
                     SGPRTGAKIDADNLTASVDRHIAQYFSAHKGTLPAAGLYGRILREVERPLILRTLAAT
                     RGNQIKAAKVLGVNRNTLRKKIRELEIQVVRSPK"
     gene            complement(12789..13907)
                     /locus_tag="VGB88_02410"
     CDS             complement(12789..13907)
                     /locus_tag="VGB88_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9646322.1"
                     /translation="MSLQNVPAMAPEARAILDALPIAVVVIGRDHRILAANPAAEQLL
                     GAGAGYLSRQRLTEIVAPDSQLVALVDQVGERGHTVSDYAVVVSGPRIGELTVDVQVA
                     PLGDDPSVIVVCLEERSMAWKLDRQLTYRGAARSVAGMAAVIAHEVKNPLSGIRGAAQ
                     LLEQFLDPPEHELTQLICQETDRIRALVERMDVFHDERPPDRERINIHTVLDHVRRLM
                     ASAAGEGVRFAEHYDPSLPPVLGNRDQLIQAFLNLVKNAVEAVPPEAGEIVISTRYQH
                     GLKFADAGGRKRQDLPILVSIQDNGPGIPEDLRNHLFDPFVTTKRDGTGLGLSLVAKI
                     VGDHGGLVEVDTRHRRTIFNVRLPAAVPGILEETWRDD"
     gene            14065..>14274
                     /locus_tag="VGB88_02415"
     CDS             14065..>14274
                     /locus_tag="VGB88_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9646323.1"
                     /translation="MFETVALIVAAGRGQRLGGAVPKQYLTLAGEPLLRRSVRAFAGH
                     PAVEAVRVVIHPADRAIYDQAVADLA"
BASE COUNT         2101 a         5089 c         4945 g         2139 t
ORIGIN      
        1 cgcccatgcc gcccatgccg ccgcccggca tcggcgcggc ctccctcttc tccgggcgct
       61 cggcgaccat cgcctcggtg gtgatcaaga gccccgccac cgaggccgcg tcctgaagcg
      121 cgatgcgcac caccttggcc gggtcgacga tgccggcctg gaccaggtcg cagtagacct
      181 ccgactgcgc gtcgaagccg aagcgctggt tcttggactc gcggaccttg ccgaccacca
      241 ccgagccgtc gacgccggcg ttctcggcga tctggcgcag cggctggccg agcgcgcgcc
      301 ggacgatgtc gacgccgacc ttctggtcct cgttctcggg attgaggccg tcgagcacgc
      361 gcgcggcgta gatcagggtc acgccgccgc ccggcacgat gccctcctcg accgcggcgc
      421 gggtcgaatg cagcgcgtcc tcgacccggt ccttgcgctc cttgacctcg gtctcggtgg
      481 cgccgccgac cttgatcacg gcgacgccgc cggcgagctt ggccaggcgc tcctgcagct
      541 tctcgcggtc gtagtcggag gtggtgtcgt cgatctgctg gcggatttgg gtgcagcggc
      601 cctcgatcgc cgcgcgcttg ccggcgccgc cgacgatggt ggtgtcgtcc ttggtgatgt
      661 tgacccgctt ggcgctgccc agcatgtcga gggtgacgct ctcgagcttg atgccgaggt
      721 cctcggagat cacctggccg ccgccgagga cggcgatgtc ctcgagcatc gccttgcgcc
      781 ggtcgccgaa acccggcgcc ttgaccgcgg cgaccttcaa gccgccgcgc agcttgttga
      841 ccaccagcgc cgccagcgcc tcgccctcga catcctcggc gatcaccaac agcggccggc
      901 tcgactgcac caccgcctcc aacagcggca gcaacgcctg caggctcgac agcttcttct
      961 cgtggatcaa cagatacgga ttgtcgagct cgcactccat cttctcggcg ttggtgatga
     1021 agtagggcga gagatacccg cggtcgaact gcatgccctc gaccacctcg agctcggtct
     1081 ccaggccctt ggcctcctcg accgtgatca cgccctcctt gccgaccttg tccatcgccc
     1141 gggcgatgaa atcgccgatc gcgcgctcgc cgttggccga gatggtggcg acctgggcga
     1201 tctcctcgct ggtcttgatc gccttggcct gcttggtgag gtgctggacc accgcctcga
     1261 ccgccatgtc gacgccgcgc ctgaggtcca tcgggttcat gcccgcggcg accgacttga
     1321 gcccctcgcg gaagatcgcc tgcgccagca cggtggcggt ggtggtgccg tcgccggcga
     1381 tgtcgctggt cttggtcgcc acctcgcgca ccatctgcgc gcccatgttc tcgaacttgt
     1441 cggacagctc gatctccttg gcgaccgtca ccccgtcctt ggtgatccgc ggcgcgccga
     1501 acgacttgtc gagcaagacg ttgcggccct tcggccccag cgtcaccctg accgcattgg
     1561 ccagaatgtc gaccccgcgc gccatgcgcg tgcgcgcatc ggtactgaac cgtacgtcct
     1621 tcgctgccat tgcctgcttc cctactccat cacgcccatg atgtcggact ccttcatgat
     1681 cagcagctcc tcgcctttga tcttgacctc ggagcccgac cacttgccga acaggatgcg
     1741 gtcgcccttc ttgatgtccg gctcgaccag cttgccgttc tggtcgcgcg ccccggcgcc
     1801 gaccgcgagc accttgccct ggctcggctt ctccttggcg gtatccggga tgatgatgcc
     1861 gccggcggtc cgctcgtctt cctcgatcct gcgcaccagc acgcgatctt gaagcggcct
     1921 gacattcatg ttgcgcctcc ctccccgata gctgccgacg cccggggccg cgggtcgggg
     1981 gaccggcctt ggcactcccg cgctcggact gctaaccatg tgccccggca tatacgaccg
     2041 ggcgtgccag gtgtcaacgg gacgccgccg ggcggtcctg cgggcttccg aaccggtcgc
     2101 cgaagcgggc gacgtccgcc ggatcgaggg cgacgcgcac gtggaccgcg gccgtgccgt
     2161 cgcgtcgcgt cagcacctcg ccgtggcggt acagccaggc cagcgccgcg ccgtcgccga
     2221 ggtcgacatc aaggtcgagc acccgccggc catgcgacag cttgcggccg atcagcgcca
     2281 gcagcgccgc gcggccggtg ccggcgagcg ccgagacggc caccgcgtcc gcgttgccgg
     2341 cgcaccgtgc ctcgagcgcc tgcctttggc cggcgtcgag caggtcgatc ttgttgtgga
     2401 cctcgaccac gcgcgcgcga tcctggtcgt cgatgtcgag ggtgcgcagc accgcccgca
     2461 cgtcgtcggc ctgcgcgccc gcgtccgcgt gggccgcatc gcgcacgtgc acgatcaggt
     2521 cggccaggat cacctcttcg agggtggcgc gaaacgcggc gatcaggtcg gtcggcaggt
     2581 cggagatgaa gccgaccgta tcggacagga tgaccgtccg cccggacggc agcacggcgg
     2641 cgcgcatggt cgggtcgagg gtcgcgaaca gccggtccgc cgcggcgacg tcggcgccgg
     2701 tgagccggtt gaacaaggtc gacttgccgg cattggtgta gccgaccaga gccaccaacg
     2761 gatagggcac gcgccggcgc gcggcgcgct ggaggccgcg ggtgcgcacc acctcggcga
     2821 gcgcgcggcg gatgcgcacg atgcgcccgt cgatcaggcg ccggtcggcc tcgagctggg
     2881 attcgccggg gccgccgatg aagccgaggg cgccgcgctg gcgctcgagg tgcgtccagg
     2941 accgtaccag gcggctgcgc tggtaggtga gcgcggcaag ctcgacttgc aggcgcccct
     3001 cggcggtgcg cgcgcgcgcg ccgaagatct cgaggatcag ggcggtgcgg tcgagcacct
     3061 tgcacgacca cgccttttca aggttgcggt gctggaccgg ggtcagcgcg gcgtcgacca
     3121 ccaccacgtc gggggggcgg ccgtcggcgg cgcgcaggcg ctcggcgatc tcggccacct
     3181 tgccccgccc gaccagggtc gacggccgcg gcgtgcgcac cggcaccacc tcggccagcg
     3241 caacctcgag gtcgatcgcg cgcgccagcc ctaccgcctc gtcgagccgc gctcgcccgc
     3301 tgcgcgtgct ggccccgggc tgccgcgccc gcccgctggc catcgccggg tgcaagaccc
     3361 acgcccgccc tgtcggaccg acgatggcgg ccgcggcgtg gccgttaacg ggtgcggtgc
     3421 gcgtcacttc gattcaggct tcctcgctcg tcttgtcagg ctcgaacaat tggaccggcg
     3481 ttcccggcat caccgtggat atggcgtgct tgtagaccag ttgcgagatg ccgtcgcgac
     3541 ggagcagcac gcagaaattg tcgaaccagg tgataacccc ttgcagcttg accccgttga
     3601 ccaggaatat ggtcaacggc gtcttgttct tgcgaacgtg gttcaggaac acgtcctgca
     3661 cgttctgggt cttgtcggcc gccatctgtg tgttcccccc tcctggttct cgctcggatc
     3721 gacgcggccc gcgcgtggcc ttccctccag gcccgcgtcg tagctatccg tcgaggaacg
     3781 cctatagcac ccgttttcac ccccccgcgc aagcccgcgc gcgcggcggc ggcgcgcgat
     3841 tccggccgcg gagctggaag acggttgctc aagcagctgt tctggaagcg aaatcgaggt
     3901 agagcgaccg caaacgctcg gtcgtggacc cgggcttgcc gttggccacc ggatgatcgt
     3961 cgatccggat caccggcacc acgaacgagc tggtgctggt caggaacgcc tcgcgcgcgc
     4021 gcctggcgtg gtcgggcgtg aacgcgcgct cggcgaccgc gaggcccgag ccgcgggcga
     4081 gcccgatcag ccgccggcgg gtgatgccgc tgaggatgtc gttgctttcg ggccgggtca
     4141 ggatggtgcc gtcggtgtcg acgatccagg cgttcgattg cgcgccttcg gtgaccaggc
     4201 cccggtcgtc gacctgccag gcctcgtacg cgccggcctc ggccgccgcc tgcttggcca
     4261 gcacgttggc caacagcgac accgacttga tgtcgcgccg gcgccagcgc aggtcgggca
     4321 cggtgatcac cgccacgccc tcggtcagcg ccgccgcagt cgccggccgc gcccggcgcg
     4381 cggtgaccac caccgacggc cgcacgccga ccgggaaggc atggttgcgg ggcgcccggc
     4441 cgcggccgat ctggagatag acgatgccct cggccacccg attgcgccgg accacctcgt
     4501 cgagcacggc gtcgagcgcg gcgcggccca tcggcgcggc gatccgcatc tcgccgagcg
     4561 agcgctccag ccgctcgtgg tgcagcgcgc gatcgatcag tcgaccgccg agcacggcga
     4621 tcacttcgta gaccccgtcc gagaactggg tcgcgcggtc ctcgatatgc atctcggcgc
     4681 cgccgtgggg caggtagcgg ccgttgacat aggcgatgcg ggacatcgca gccctcgttt
     4741 cagcgttgcc agaactccgg cgttaatagc atcagcaccg cgaacagctc cagccgcccg
     4801 accagcatgc cgagcgcgag cagccagtgc gcggtctcgg gcaaggaggc gagcgagccg
     4861 ccgagacccg cggcgccggt gagcagcggc ccggtgttgg cgatcgcgct gagcgcaagc
     4921 agcatcgacc gctcggcatc gaggccgacc gcggcgaggc cgagcgcaag cgtgatcagg
     4981 caggccagga acaggacgaa gtaggcccac accgcctcca tcgccgaatc ggtgatcgag
     5041 gtgcggccga gcttgatcac caccaccccg tgcgggtgcg ccagccgctc gagctcgcgc
     5101 acgctctggc gcagcagcag cagggcgcgc cccagcttga tgccgccggc ggtcgaggtc
     5161 gacgcgccgc cgaccagcac cagcccggcc agcaccagga cgacgaacac cggatcgggc
     5221 cccgagtccg cgccggcgcc gtagccggtg gtagtggccg cggagacggc ctgaaacagc
     5281 gcgcgacgca gctcggcgcc gatcgcgcct gcgccttccg cgacgccgag cacggcgagc
     5341 agcacggcgg cggtggccac caccagcagc atgaacccgg tctcgggatc ggcgccatag
     5401 gcgcgcgggc ggccgcacag cgccgcccaa tggacggtga agttgatcgc acccgcggcc
     5461 atgaacagga tcagcaccat ctcggccatg ccgttgccga gcgcgagagg cccgccgcgg
     5521 tcggggctga agccgccggt tgacacggtg ctcagcgcgt agcatgcggc gtggaagctg
     5581 gtcaggccgg tggcggcgag cgccatgaag cacaggacgg tgaggaccgc atagggcacc
     5641 agcaccgcct gcgcggcctg gaaaaagcgc gggcggaacg gctcggccag ccgctcgggc
     5701 gacgacgcgc cggcgcggcg ggcgcgcgtg gcgaacccga cctgggggat cagggtggcg
     5761 gcgaacacca acgtcgctgc gccgccgatc cattgcagaa gggcgcgcca caataccacg
     5821 ctgcggccgg cgccggccgg gtcggcgatg acgctgagcc cggtcgtggt caggccggag
     5881 accgcttcga gcaccgcccc cggcgcgttc ggcgcgaccc cggcaagcgc gaacggcagc
     5941 gccgcgaaca gcgacaggcc ggcccagccg agcgccgcca gcaccagcgc gtcgcgccgg
     6001 gtcacccgcc gccaggtgcc gcgcccgacc agcagcaaga ccacgccgaa gaacaacgcc
     6061 gtgcccgcgc ccagaccgaa ggcgcgcaca tccgccgcct cgccgagcgc aagcgccacc
     6121 gcgagcggcg caagctcggc cgcggcgagg ccgaccagca atgccccgag cagggccagt
     6181 atcgtggccg cggccatgat ccgagcccct gcccgccgac gccggcctca gaagaagccg
     6241 agggcgaccg cgaacatccg ctcgaccttc ttcaccgcat cgtggggggc gaagatgatc
     6301 acccggtcgt gctctttgac cgagtttgcg gcgcgcggaa tgatcacctc ctcgccgcgc
     6361 acgatcgccc cgaccagcac gcccttcggc agcttgacgt ccttcaaggg cgggccgacc
     6421 agcggcgagg tcgccatcgc ctcggcctcg atcacctccg cctcgccgcc gcgcaacgaa
     6481 tagaccgagc ggatgcgccc gcggcggatg tgctggagga tgctggacac ggtaatgccg
     6541 cgcgggctta ccgaggcgtc gatgccgagc ggcgagatca gcggcgcata ggtgctcgag
     6601 ttgaccaggg tcacggcgcg ggcgcagccg tgccgcttgg ccagcaccga ggcgagcaca
     6661 ttgacctcgt cgttggcggt gacggcaagc acggtctcgg tattggcgat gttggcttct
     6721 ttgaggatgt cggtgtccag cgcgtcgccg ttgatcacga tggtgcgctc gagccggtcg
     6781 gcgacgaact cggcgcgctc cttgtcgaac tcgatcagct tcaggttgat gttgggattg
     6841 gtcttctcga tctggcgtgc cagcagcagc ccgatattgc cgccgccgat gatgaccagc
     6901 cgccgcgccg cctgttcctc gtggccgaac aggaccatgg cgcgggcgag ctgcgcggtc
     6961 tcggcgacga agtagacctc gtcgcccgcg accagctgct ccatgcgctc gggaaacagg
     7021 gtcacctcgt tgcgcacgat gcacacgatg gtgatgttga ggtcggggaa cagctcgttg
     7081 agctggccga gcggcgtgtt gaccaccggg caggactcgc caagccgtac gcccaccagc
     7141 cgcacccgat cgtcggcgaa cgaggtcatg tcgagggcgc cggggacctc gagccgccgg
     7201 ccgatcgagt tggccacctc gacctcgggc gagataacca cgtcgatcgg catatgcgcg
     7261 cggctgaaca ggtcggccca ggccggatcg agataggcct gatggcggat gcgcgcgatc
     7321 ttggtcggca cgttgaacag cgagtgcgcg acctggcagg cgacgatgtt gatctcgtcc
     7381 gactgggtca ccgcgatcag catgtcggcg tcctcggcgc cggcgagctt gagcacgtcg
     7441 gggtgcgagg cgaaaccgag catcgcctgc acgtcgagcg cctcgccgac cttctcgatg
     7501 cgctcgggcg cctggtcgat gaccacgacg tcgttgtcct ccgccgccag atgccgggcg
     7561 atgttgaagc cgacctcacc ggcgccgcag acgagcactc tcaactcaaa cgctccgatc
     7621 gggtgttgcg ggggcggtgc cgggcgggct cgcgccccgc cctacctttc gtcgccggtg
     7681 atgccgagcg acttgagctt tcggtgcagc gccgagcgct ccatgccgac gaacgacgcg
     7741 gttcgcgaga tgttgtgccc gaaccgggtc acttgcgcct gcaaatattg ccgttcgaac
     7801 atctccctcg cctcgcgcag cggcagggtc atgatctcgg ccccgttctc cgatcgcatc
     7861 gccgccggtg tcgcgaccag cagctcgggc ggcaacatgt cggcggtcac cggctcggcg
     7921 atgtcgccgg gcgccatgat caggatccag tcgaccaggt tgcgcagctg gcgcacgttg
     7981 cccggccagt cgcaggtctg cagcaccgcc atcgcgtccc cggccagtgc gcgcccggcc
     8041 aggccgtagg cgccggcctt ggagacgaaa tggctgacca gctgcgggat gtcctcgcgc
     8101 cgatcgcgca acggcggcac ccggatcgga accacgttga gccgatagaa caggtcctcg
     8161 cggaacttct tgttggtgat ctcggccggc aggtccttgc tggtcgaggc cagcacgcgc
     8221 acgtcgacct gtatgttgcg gctgccgccg acgcggcgga aggtctgctc ctgaagcacg
     8281 cgcaggatct tgccctgggt ctgcaacggc atgtcggcga cctcgtcgag cagcagcgtg
     8341 ccgccatggg cctgctcgaa taccccgatc ttgcgcggac ccgtctcgtt ttcgcccgcg
     8401 ccctcgaccc cgaacagctc ctcttccatc cgctccggcg tcatcgccgc ggcgttgatc
     8461 accacgaacg ggccgttggt gcgccgggac cggccgtgga gcaggcgcgc caccacctcc
     8521 ttgcccgagc cggcgggacc cgagatgagc acgcggctgc cggtcggcgc gaccttgtgg
     8581 accgcctggc gcagcaccga catggcgtgg ctgtcgccga tcagcacgga ctcggtgacc
     8641 gcacgctggc gcagctcggc gttctcgcgc cgcaggcgcg aggcctcgag cgcgcgctcg
     8701 acgctgagca gaagccgatc cgcctcgaac ggcttctcga tgaagtcgta ggcaccgagc
     8761 cggatcgcct tgaccgcggt ctcgatattg ccgtggccgc tgatcatcac caccgcgagc
     8821 tcgggatagc gcgccctgat ctgctgcaac agcgcaatgc cgtccagccg gctgccttgc
     8881 agccagatgt cgagcagcac caggcccgga agccgggcgc cgatctcgct cgccgcgctg
     8941 tcggcgtccc acgcctcgcg cgtctggaag ccctcgtcgg cgaggatgcc ggcgatcagg
     9001 gaacggatgt cccgctcgtc gtcgacgatc aatacttcac tggtcatcga accggttcga
     9061 ccgcgtccac accagggcct tgcgaggctc ttgtcgagga tcggcgcgga aacacgagga
     9121 gtgcgcgggc gccacccatt gagccgtcgg tcagctgcac ctcgccacca tgatcctcca
     9181 tgatcttctt gacgatggca agaccgagcc cggtcccgcg ctcgcgcgtg gtgacgtagg
     9241 gctcggtcag ccggctgcgc tgctccagat cctgcggcag ccccctgccg ttgtcctcga
     9301 tcgccaccgt cagccgctcg gccgaggacg catcgatgcg caccgcgacc ctgcccgggg
     9361 gcaaggcggc ggcgttggcg gcgcgcccgt cgatggcgtc gatcgcgttc tgaagcatgt
     9421 tggtcatcgc ctggccgacc tgcgcggcgt cgcaatgcag cgccaccggc ggcaccggcg
     9481 cctcgacctc gaagccgatc tcgggatggg catgccgttg caaggcgacc gccgccgcga
     9541 cgatcccggt caggtcggcc tcgaccagca ccggcgccgg catgcgggca aaggccgaga
     9601 actcgtcgac catgccgcgg atcgcgccca cctggcgcac gatcgtgtcg acgcagtccg
     9661 agaacacctg ctcgccctcg cccagccgtg cccggtactt gcggcgcagg cgctccgccg
     9721 acagctggat cggggtcagc gggttcttga tctcgtgggc gatacggcgg gcgacgtccg
     9781 cccaggccgc ggcgcgctgg gcatggacca ggtcggtgat gtcgtcaaaa gtgacgacgt
     9841 agccggtgat gcgcccgtgc gtgcgctggg cgccgatccg caccagcagc tcgcgcgccc
     9901 ggttgtcgcg gatgatcgac accttgtcct gcacgtggcc ggccgagccg gcgcgcaggt
     9961 cggccatcaa gtcggccatc tccggcacgg cacgctcgag cggctggccg acgagcgagt
    10021 ccgcgctgac cgccaggagg tcgagcgcgg tctggttggg caggaagatc cggccgtcgc
    10081 ggtcgagccc cagcacgccg gccgagacgc cgtacagcac cgcctcggtg aaccggcggc
    10141 ggttgtcgat ctgcgtgttg gcctcgatca gctcggtacg ctggctttcg agctggccgg
    10201 tcatgcggtt gaacgcccgg ctcagcgccg cgatctcgtc gccgacatgg ccctcggcga
    10261 cgcgcacgga aaggtcgccc gcgcgcaccc gctcggtcgc gccgaccagc gccgagatcg
    10321 gccggatcaa tcggttggtg aacaccagcc cgatccatac cgcggccagc agcagcagca
    10381 gggcgacgac gacatagagc agggtgaagg tgatctggat tcccgaccgc cgggcctcga
    10441 gcgccttgta gtcggacacc gcgctggaca ggcgcaccat gtggttgagc acctccgcgt
    10501 cgacgaagcg gccgacatag aggaacgcgc cgaccatgcc gtcgatgcgc gccagcgcgc
    10561 ggatgcgatc gctctcgtcg ctggtcatga tcaccagctc gccctcggcg gcgcgctgca
    10621 acgccgcatt ggcgatcggg tcgaactcga gcacgaagct cagccggctg cgcgccagga
    10681 tcttgccgtc gccgcgcacg atgatcgcct cggacagcga gcgctcgccg gccatcgcat
    10741 cgagcaggcg gttgagctct tccggcgagc cgagcaccgc ggagtcctgg cggttgagcg
    10801 tcgcgaccat cgacaggatg tcggcgcgga tgaactcgcg gtgatccttg agatatgcct
    10861 ccgacacggc cagcgaccgg tcgagcgcgg tgcgcacgcg gtcgttgaac caagtctgga
    10921 tgccgaggtt gaagaacgtc gccgagaaca cggcgacgac gatcgccggc gccaccgcga
    10981 ccaggctgaa catggcgacg atgcgcgcgt gcaggcgcga tccgaccgag ccctggcgcc
    11041 gctcgatcca cagccgcacc agatgccagg cgaccagggc gccgagcagc agcagcagca
    11101 ccaggtcgat gttgagcatg atcaggacgg tcgtcaccgt gccgccgtcg ggcgacatcg
    11161 acccggtcag cagcacgtaa gtggcgatgc cgaagatgcc cgccgcgatc gcgaccgcga
    11221 tcgccagcct gcgagtcacg ctcgggcgca gcggccagcg gggcgcgagc ctgcgccagc
    11281 cggcgggcgc cgcgggcgag gagtcgatgc tcatggcgat gcctcggtca cgggccgggg
    11341 cctatttcgg cgaccgcacg acctggatct cgagctcgcg gatcttcttg cgcagggtat
    11401 tccgattgac gccaaggact ttcgccgcct tgatctggtt gccgcgggtc gccgccaagg
    11461 tgcgcagtat caggggtcgt tcgacctcgc gcaggatgcg accgtacagg cccgctgcgg
    11521 gcagtgtacc cttgtgggcg gaaaaatact gcgcgatgtg gcggtcgacc gaggcggtca
    11581 ggttgtcggc gtcgatcttg gcgccggtgc gcggcccgct gcggccgtcg gatagctcgg
    11641 cggcgacgat cggctcggtg atgacctcct cggcgtaaag cgccgccagc ctgcgcacca
    11701 ggttctcgag ctcgcgcacg ttgccgggcc agccatacga cttgagccgc gccatcgcct
    11761 ccggcgtcac cgacttcgcc ggcaggccct cgtcctgggc gcgggcgagg aagtgcttga
    11821 ccaggatcgg cacgtcgtcg gcccgctcgc gcagcggcgg caggcgcagc ggcaccacgt
    11881 tgaggcgata gaacaggtcc tcgcggaaca ggccgcgctc gatcagctgg gtcaagtcgc
    11941 ggtgggtggc ggcgatgatg cgcgcgtcgg tgcggatcgg ctggcggccg ccgatggtgg
    12001 tgtactcgcc ctgctggagc acccgcagca ggcgcgtctg cgcctcgggc ggcatgtcgc
    12061 cgatctcgtc gaggaacagg gtgccgccgt tggcctgctc gaagcggccg atcgagcgcc
    12121 cgctggcacc ggtgaacgcg ccgcgctcgt ggccgaatag ctcgctctcg atcagctcgc
    12181 gcgggatcgc cgccatgttg atcgccacga acggcatgcc gcgccgcttg ccgtagtcgt
    12241 gcagcgcgcg cgcgaacagc tccttgccgg tgcccgactc gccgctgatc atgaccgtga
    12301 ggtcggtgcc catcaggcgc gccagcatgc ggtagacctc ctgcatcgcc ggcgagcggc
    12361 cgaccagcgg caggccctcg tcgggctggt cggcggccgc agccggccgg gcgagctcgc
    12421 cctcgggcgg ttcggccagc gcgcggcgga caaccgcgat cagctcgttg aggtcgaacg
    12481 gcttgggcag gtactcgtag gcgccgcgct cgttggcgcg gatcgcggtc agcagcgtgt
    12541 tgcgcgcgct catcaccacc accggcaggt cggggcggac gctcttcagt cgcggcagca
    12601 gctcgagccc gtcgtcgtcg ggcaggacca cgtcggtgat caccagatcg ccctcgccgc
    12661 cgttgaccca gcgccacaaa gtcgccgccg tgccggcgcc gcgcaccgta tagccgagcc
    12721 tgccgagcgc tttgccgagc acggtgcgga tggagcgatc gtcgtccgcg atcagaatcg
    12781 tcgccggctc agtcatcgcg ccaggtctcc tcaaggatgc ccggcaccgc cgcaggcagg
    12841 cggacgttga agatggtccg gcggtgccgg gtgtcgacct cgaccaggcc gccgtggtcg
    12901 ccgacgatct tggccaccag cgacagcccg agcccggtgc cgtcgcgctt ggtggtcacg
    12961 aacgggtcga acaggtggtt gcgcaggtcc tccgggatgc ccggcccgtt atcctgtatc
    13021 gacaccagga tcggcaggtc ctgacgcttg cgcccgccgg cgtcggcgaa cttgaggccg
    13081 tgctgatagc gggtcgagat cacgatctcg ccggcctccg gcggcactgc ctcgacggcg
    13141 ttcttgacca ggttcaggaa cgcctggatc agctggtcgc ggttgccgag caccggcggc
    13201 agcgacggat cgtaatgctc ggcgaagcgc acgccctcgc ccgccgcgct cgccatcagc
    13261 cggcggacat ggtcgagcac ggtatggatg ttgatgcgtt cgcggtccgg cggccgctcg
    13321 tcatggaaca cgtccatgcg ctcgaccagc gcccggatgc ggtcggtctc ctggcagatc
    13381 agctgggtca gctcgtgctc gggcgggtcc aagaactgct ccagcagctg ggccgcgccg
    13441 cgaatcccgg acaacgggtt cttgacctcg tgggcgatca ccgccgccat gccggcgacc
    13501 gagcgcgccg cgccgcgata ggtcagctgg cggtcgagct tccacgccat cgaccgttcc
    13561 tcgaggcaga ccacgatcac cgagggatca tcgccgagcg gcgccacctg gacgtcgacc
    13621 gtaagctcgc cgatgcgcgg ccccgacacc accaccgcgt agtcgctgac cgtatggccg
    13681 cgctcgccga cctggtcgac cagcgccacc agctggctgt cgggggcgac gatctcagtc
    13741 agccgctggc ggctcagata gccggcgccg gcaccgagca gctgctcggc cgccggattg
    13801 gcggcgagaa tccggtggtc gcggccgatc accaccaccg cgatcggcag cgcatcgagg
    13861 atcgcccgcg cctcgggcgc catcgcgggg acgttttgca gcgacatggg acgactttgc
    13921 ttccggcctg cccaaaagtt aggcattact taaaggcaag cgccggcgcg tgcaacggga
    13981 atgcgccacg ccggcctccc cgggcgcgcc ttggcaaccg gatcggcgcg gagtaccgtg
    14041 ccgccggccg atggacgctg cgccatgttc gagaccgtcg ccctgatcgt cgctgcggga
    14101 cgcggccagc ggctgggcgg ggcggttccc aagcaatatc tgaccctcgc gggcgagccg
    14161 cttctgcgcc gctcggtgcg cgccttcgcc ggccacccgg cggtcgaagc ggtgcgggtg
    14221 gtgatccacc cggccgatcg cgcaatctat gaccaggcgg tcgccgacct cgcc
//
LOCUS       DASWHU010000064         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2242884, whole genome shotgun sequence.
ACCESSION   DASWHU010000064 DASWHU010000000
VERSION     DASWHU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242884"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /gene="pbpC"
                     /locus_tag="VGB88_02420"
     CDS             complement(<1..1335)
                     /gene="pbpC"
                     /locus_tag="VGB88_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX9646324.1"
                     /translation="MGVWCLALVLAGLDGLDRAYPPPLAQMWPQSVMVVDRDGGLLRA
                     FATPAGRWRLPVRLAEVDADLLRMLIAYEDSRFHAHPGVDPLAMLRAAGQLAWNGRIV
                     SGGSTLTMQVARLLEPRQHRSFDAKIRQAVRAVQLEWALGKDEILGLYLTLAPYGGNL
                     EGLRAASLAYFSKEPRRLTLGEAALLVALPQSPEYRRPDRYPEAAKRARDRVLDRIAG
                     HGLFSDAEIAHAKDEKVPTARQPMPLIAPHAADQAHTGAPNERELKLTIDAGLQKILE
                     SLARDRAQALGPDMSVAMVVVDNATGEVMARVASPDYFDERRAGQVDLTQAVRSPGSA
                     LKPFIYGLGFEDGLIHPESLIEDRPIRYAGYQPENFDLTFQGTVTVRRALQLSLNVPA
                     VAVLDEVGPSRLIARLAEAGAPLVLPRREAPGLALGLGGVGVRLTDLTMLYAG"
     gene            complement(1404..>1602)
                     /locus_tag="VGB88_02425"
     CDS             complement(1404..>1602)
                     /locus_tag="VGB88_02425"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646325.1"
                     /translation="FRSDRFIAAFDRSAEDERSFALAYMVRAVTPGRYVHPAAQVEDM
                     YRPHLFARTEEGRAEVVGPQQ"
BASE COUNT          242 a          566 c          565 g          229 t
ORIGIN      
        1 gcccgcatag agcatggtga ggtcggtgag cctcacgccc acgccgccaa ggccgagcgc
       61 gagccctggc gcttcgcggc gtggcagcac gagcggcgct ccggcctcgg cgaggcgcgc
      121 gatgaggcgg ctcggtccca cctcgtcgag cacggcgacg gccggcacgt tcagcgacaa
      181 ttgcagcgcg cggcgcaccg tgacggtgcc ctggaaggtg aggtcgaaat tctccggctg
      241 atagccggca tagcggatcg gcctatcctc gatgagactc tccgggtgga tgagcccgtc
      301 ctcgaagccg agcccataga tgaacggctt cagcgccgag cccggcgaac gcacggcttg
      361 cgtcagatcg acctgaccgg cgcgccgttc gtcgaaataa tcgggcgagg cgacccgcgc
      421 cataacctcg ccggtggcgt tgtcgaccac caccatcgcc accgacatgt cgggcccaag
      481 cgcttgcgcc cggtcgcgcg cgaggctctc caggatcttc tgcaggccgg catcgatggt
      541 cagcttgagc tcgcgctcgt tgggggcgcc ggtatgcgcc tggtcggcgg catggggcgc
      601 gatgagcggc atgggctggc gcgcggtcgg caccttctcg tccttggcgt gcgcgatctc
      661 ggcgtcgctg aacaggccgt gccctgcgat gcggtcgagc acgcggtcgc gcgcgcgttt
      721 tgccgcttcg gggtagcggt cgggcctgcg gtattccggc gactggggca acgccacgag
      781 cagcgccgct tcgccgagcg tgagacggcg cggctccttg ctgaaatagg ctaacgacgc
      841 cgctctcaac ccttcgagat tgccgccata cggcgcgagc gtgagataga gcccgagaat
      901 ctcgtccttg ccgagcgccc attcgagctg cacggcgcgg accgcttgcc tgatcttggc
      961 gtcgaaagat cgatgctgac gcggctcgag caggcgcgcg acctgcatgg tgagggtgga
     1021 gccgcccgag acgatgcggc cgttccaggc gagctggccg gccgccctca gcatcgccag
     1081 aggatcgacg ccgggatggg catggaagcg gctgtcctcg taggcgatca gcatgcgcaa
     1141 caggtcagcg tcgacctcgg cgagcctgac cggcagccgc cagcgcccgg cgggcgtcgc
     1201 aaaggcgcgc agcagcccgc cgtcgcggtc gaccaccatc accgattgcg gccacatctg
     1261 cgcaagcggc ggtggatagg cgcggtcgag cccgtcgagc ccggccagca caagggcgag
     1321 gcaccacacc cccatgcccg ggcccaccac ccaacgccgg ggccagcccc cggccccggc
     1381 gcgccccggc tcgctgctgg cgctcactgc tgcggtccca ccacctccgc gcgaccctcc
     1441 tcggtgcgcg cgaacaggtg cggacggtac atgtcctcca cctgggcggc cgggtgcaca
     1501 tagcgcccgg gtgtcactgc gcgcaccatg tacgccagcg cgaagcttcg ctcgtcctcg
     1561 gcgctgcggt cgaaggcggc gatgaagcgg tcgctgcgga ac
//
LOCUS       DASWHU010000065         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1498130, whole genome shotgun sequence.
ACCESSION   DASWHU010000065 DASWHU010000000
VERSION     DASWHU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498130"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGB88_02430"
     CDS             <1..282
                     /locus_tag="VGB88_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9646326.1"
                     /translation="RMDEVIFEEFKGTGNSEIVLDRKLADKRTFPAIDIGKSGTRKEE
                     LLVQKGELSKMWVLRRILTPMGVTDAMEFLIDKLKGSKSNQDFFDSMNQ"
     gene            complement(401..1381)
                     /locus_tag="VGB88_02435"
     CDS             complement(401..1381)
                     /locus_tag="VGB88_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX9646327.1"
                     /translation="MKCHAIQADKTGGPEVLAWREVEVGDPGPGEILVRHQAVGLNYI
                     DTYQRSGLYPPATKPFTLGLEGAGVVEKLARDVTGLKAGDRIAYASPPMGAYAQLRLM
                     PADRVVKVPDGIDHDTAAAMMLKGMTAEYLLRRTYPVKKGDVILFHAAAGGVGLIACQ
                     WAKAIGATVIGTVGTDQKAELARAHGCDHPIVYTREDLVARVKEITGGEGVAVVYDSV
                     GKDTFERSLDCLSPRGLLALFGQSSGPVGPFDPAVLAAKGSLYLTRPSLMVYNAKRAD
                     LEASAAALFDMVGSGKVKIEINNRYPLAKAAQAHRDLEGRKTTGSTIFEP"
     gene            complement(1461..1931)
                     /locus_tag="VGB88_02440"
     CDS             complement(1461..1931)
                     /locus_tag="VGB88_02440"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9646328.1"
                     /translation="MMSSAVYDCPVGRLGVAADGDVVCAIDWVSADRSVGPLTPVLRA
                     ALDQLDAFFAGRLTRFDLPLAPAPTAFARNLRAAMTAIPFAATASYGELARALASTAR
                     AIGAGCGANPLPIVVPCHRVVGAGGRLGGYSGGGGAVTKRALLAHEQAQAALLS"
     gene            complement(1928..3976)
                     /locus_tag="VGB88_02445"
     CDS             complement(1928..3976)
                     /locus_tag="VGB88_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9646329.1"
                     /translation="MAENLLARETSPYLLQHKDNPVAWMAWGPAAFARARHENKPVLL
                     SIGYAACHWCHVMAHESFEDADIARLMNELFVSIKVDREERPDLDTIYQTALAMLGEH
                     GGWPLTMFLNAAGEPFWGGTYFPPAPRYGRPGFADVLRTMHRVYTEDSPRVQGNVEAL
                     RGGLARLGRSHPGAPLETARLDALAERLAGEVDHTLGGLQGAPKFPQVPLFALLWRAY
                     LRTGEPAYARAVLVTLDRICQGGIYDHLGGGFARYSVDPLWLVPHFEKMLYDNALLIE
                     LLTLVWQQAQTPLFEARVRETVGWVLAEMVTAGGAFASSLDADSEGVEGRYYVWTEDE
                     IDAALGADARAFKAAYDVSAGGNWEGVNILNRLRVPRLADEADEARLAGLRATLRAVR
                     ARRPRPGFDDKVLADWNGLMIAALAEAGFAFAQAEWLAAARRAFEFVSADMAQGDRLC
                     HAHRLGRAGEAGFLDDYANMARAALALYEVEGDVRDLDRARAWTAVADAHFWDDEAGG
                     YFFVADDGEALIARAKTAADTATPSGNGTMLGVLARLHYLTGDAAYRDRADRLVEAFT
                     GQVDSASAAMATVLNSFELLDAACQIAVIGDRNSSRAKAFLDIVRTSSVPGRVLQVVA
                     PGDNLPIGHPARGKAQLDNKPTVYVCVGQTCSLPIIEPEALRAALARPQAGNGAAAAP
                     "
     gene            4087..4422
                     /locus_tag="VGB88_02450"
     CDS             4087..4422
                     /locus_tag="VGB88_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX9646330.1"
                     /translation="MSRDDFYYRQHVFCCTNQRDPSDPRGCCQARGAIELRNYMKAKA
                     KKMGLEDVRINASGCLDRCELGPTMVIYPDGVWYSCKTREDVDEVLQAHLVEGRVVAR
                     LRLPLRAPG"
     gene            4419..>4714
                     /locus_tag="VGB88_02455"
     CDS             4419..>4714
                     /locus_tag="VGB88_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9646331.1"
                     /translation="MSAAIVSDETIFALSSARGAAGVAVVRVSGARARGAVEALTAKA
                     PPPPRRATLARLHAPGGEALDSALVLWFPGPASFTGEDVAELHLHGGRAVVAGVL"
BASE COUNT          669 a         1654 c         1728 g          663 t
ORIGIN      
        1 cgcatggacg aggtgatctt cgaggagttc aagggcaccg gcaactccga gatcgtgctc
       61 gaccgcaagc tcgccgacaa gcgcaccttc ccggcgatcg acatcggcaa gtccggcacc
      121 cgcaaggagg agctcctggt gcagaagggc gagctgtcga agatgtgggt gctgcgccgg
      181 attctcaccc cgatgggggt gaccgacgcg atggagttcc tgatcgacaa gctcaagggg
      241 tccaagtcga accaggactt cttcgactcg atgaaccagt agcgcgcggc gcgggccggg
      301 ctcgcgcgca gccgcggagg cgcggcagtc gccgtgccgc gccttccgcg tcgccgcgtg
      361 aaagcctgcg cctctgcgtg ccgcgtgacc gcggcggcga tcagggctcg aagatggtcg
      421 agccggtggt cttgcgtccc tcgaggtcgc gatgcgcctg ggcggccttg gccagcgggt
      481 agcggttgtt gatctcgatc ttgaccttgc ccgagccgac catgtcgaac agcgccgccg
      541 cgctcgcctc aaggtccgcg cgcttggcgt tgtagaccat caggctcggg cgggtgagat
      601 agagcgagcc cttggcggcg agcaccgccg ggtcgaacgg ccccaccggc cccgaggact
      661 ggccgaacag cgccagcagc ccgcgcgggc tgaggcagtc gagcgagcgc tcgaaggtgt
      721 ccttgccgac cgagtcgtag accaccgcca cgccctcgcc gccggtgatc tccttgaccc
      781 gggcgaccag gtcctcgcgg gtgtagacga tcgggtggtc gcagccgtgg gcgcgcgcga
      841 gctcggcctt ctggtcggtg ccgacggtgc cgatcacggt ggcgccgatc gccttcgccc
      901 actggcaggc gatcaggccg acgccgccgg cggcggcgtg gaacaggatg acatcgccct
      961 tcttgaccgg ataggtgcgc cggagcaggt actcggcggt catgcccttg agcatcatcg
     1021 ccgccgcggt atcgtggtcg atgccgtcgg ggaccttgac cacccggtcg gcgggcatca
     1081 ggcgcagctg cgcgtaggcg cccatcggcg gcgaggcgta ggcgatgcgg tcgcccgcct
     1141 tcagccccgt gacgtccctg gccagcttct cgaccacgcc ggcgccctcg aggccgaggg
     1201 tgaacggctt ggtcgcgggc ggatagagcc ccgagcgctg gtaggtgtcg atgtagttga
     1261 gcccgaccgc ctggtggcgg accaggatct cccccggacc ggggtcgccg acctcgacct
     1321 cccgccaagc cagcacctcg ggaccgccgg tcttgtctgc ctgaatcgcg tggcacttca
     1381 tggcttgctg atctccctcc cggttggatc gaacctggcc cctggggcac ggcccactct
     1441 agcgggcgcc cgcgggcgtg tcatgacagc agcgcggcct gcgcctgctc gtgggcgagc
     1501 agcgcgcgct tggtgacggc gccgccaccg ccgctgtagc cgccgaggcg gccgccggct
     1561 ccgaccacgc ggtggcacgg caccacgatc ggcagcgggt tcgcgccgca gccggcgccg
     1621 atcgcgcgcg cggtgctggc gagcgcgcgg gcaagctcgc cgtagctcgc ggtcgcggcg
     1681 aacgggatcg ccgtcatcgc cgcgcgcagg ttgcgggcga acgcggtcgg cgccggcgcg
     1741 agcggcaggt cgaaccgggt cagccgaccg gcgaagaagg cgtcgagctg gtcgagcgcc
     1801 gcgcgcaaca ccggcgtgag tggcccgacc gagcgatcgg ccgagaccca gtcgatggcg
     1861 cagaccacgt cgccgtcggc ggccacgccc aggcgcccga ccgggcagtc gtaaacggcc
     1921 gacgacatca tggtgcggcc gccgcgccgt tgccggcctg cggccgggcg agtgccgcgc
     1981 gcagcgcctc gggttcgatt atcggcagcg agcaggtctg gccgacgcaa acatacacgg
     2041 tcggcttgtt gtccagctgc gccttgccgc gagccggatg accgattggc aggttgtccc
     2101 caggcgccac cacctgaagc acccgaccgg gcaccgagct ggtccggacg atgtcgagaa
     2161 aagcctttgc gcggctggag ttgcggtcac cgatcacggc gatctggcag gcggcgtcaa
     2221 gcagttcaaa actgttcaag actgtcgcca tcgccgcgct cgcgctgtcc acctgtccgg
     2281 taaaggcctc gaccaaacga tcggcccggt cgcggtaggc ggcatcgccg gtcaggtaat
     2341 ggaggcgcgc gagcacgccg agcatggtgc cgttgccgga cggcgttgcc gtgtccgccg
     2401 cggtcttggc gcgcgcgatc agggcctcgc cgtcgtcggc gacgaagaag tatccgcccg
     2461 cctcgtcgtc ccagaagtgc gcgtcggcga ccgcggtcca ggcgcgtgcg cggtcgaggt
     2521 cgcgcacatc gccctcgacc tcgtagagcg ccagcgcggc gcgcgccatg ttggcgtagt
     2581 cgtcgaggaa gccggcctcg cccgcacgcc ccagccggtg ggcatggcac agccggtcgc
     2641 cctgcgccat gtcggcagag acgaactcga acgcgcgccg ggcggcggcg agccactcgg
     2701 cttgcgcgaa cgcgaacccg gcctcggcga gcgccgcgat catcagtccg ttccagtcgg
     2761 cgagcacctt gtcgtcgaag cccgggcgcg ggcgccgcgc gcgcaccgcg cgcaaggttg
     2821 cgcgcaatcc ggcgagccgc gcctcgtcgg cctcgtcggc cagccgcggc acgcgcagcc
     2881 ggttgaggat gttgaccccc tcccagttgc cgccggcgga gacgtcgtag gcggccttga
     2941 aggcgcgcgc gtcggcgccc agcgcggcgt cgatctcgtc ctcggtccag acgtagtacc
     3001 tgccctcgac gccctcgctg tcggcatcga ggctggaggc gaaggcgccg ccggcggtga
     3061 ccatctcggc cagcacccag ccgacggtct cgcgcacccg cgcctcgaac aagggcgtct
     3121 gcgcctgctg ccagaccaag gtcagcagct cgatcagcag cgcgttgtcg tagagcatct
     3181 tctcgaaatg cggcaccagc cacagcgggt cgaccgagta gcgcgcgaag ccgccgccca
     3241 gatggtcgta gatgccgccc tggcagatgc ggtcgagcgt caccagcacg gcgcgcgcgt
     3301 aggccggctc gccggtgcgc aggtacgccc gccagagcag cgcgaacagg ggaacctgcg
     3361 ggaacttggg cgcgccctgg aggccgccga gcgtgtggtc gacctcgccc gccaggcgct
     3421 cggcgagggc atcgagccgc gccgtctcca gcggcgcgcc ggggtgcgag cggccgagcc
     3481 gggcgaggcc gccgcgcaac gcctcgacat tgccctgcac ccggggcgag tcctcggtgt
     3541 agacccggtg catggtgcgc agcacgtcgg cgaagccggg ccggccgtag cgcggcgccg
     3601 gcgggaagta ggtgccgccc cagaacggct cgccggcggc gttcaggaac atggtcagcg
     3661 gccagccgcc gtgctcgccg agcatcgcca gcgcggtctg gtagatggtg tcgaggtccg
     3721 ggcgctcctc gcggtcgacc ttgatgctga cgaacagctc gttcatcagc cgggcgatgt
     3781 cggcgtcctc gaagctctcg tgggccatta cgtggcacca gtggcaggcg gcatagccga
     3841 tcgacagcag gaccggcttg ttctcgtgcc gcgcgcgcgc gaacgccgcc ggcccccagg
     3901 ccatccaggc gacagggttg tccttgtgct ggagcaagta cgggctggtc tcgcgggcga
     3961 gcaggttctc ggccatcgcc tcccctcggg cctggttgcg ccggcccccg gcaccgacta
     4021 acgtgcgcgc ggccgcgcag cgggcggcct ctgggtatac agcctcggcc ccacgggaga
     4081 aaaggcgtgt cgcgggacga tttctactac cgccagcacg tgttctgctg caccaatcag
     4141 cgcgatccga gcgacccgcg cggctgctgc caggcgcggg gcgccatcga gcttcgtaac
     4201 tatatgaaag caaaggcaaa aaagatgggg ctcgaggacg tgcggatcaa cgcctcgggc
     4261 tgcctcgacc gctgcgagct ggggccgacc atggtcatct atccggacgg ggtgtggtac
     4321 agctgcaaga ccagggaaga cgtcgacgag gtgctccagg cgcatctggt cgagggccgg
     4381 gtcgtcgcgc ggctgcggtt gccgctgcgc gcgccgggct gagcgccgcc atcgtttcgg
     4441 acgaaaccat cttcgcgctg tcgtcggcgc gcggcgccgc cggcgtggcg gtggtgcgcg
     4501 tctccggcgc gcgcgcgcgg ggcgcggtcg aggcgctcac tgcgaaagcc ccgccgccgc
     4561 cgcggcgcgc cacactcgcc cggctgcacg ccccgggcgg cgaggcgctc gatagcgcgc
     4621 tggtgctgtg gttcccgggc ccggcgagct tcaccggcga ggacgtggcc gagttgcacc
     4681 ttcacggcgg ccgggcggtg gtcgccggcg tgct
//
LOCUS       DASWHU010000066         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3350375, whole genome shotgun sequence.
ACCESSION   DASWHU010000066 DASWHU010000000
VERSION     DASWHU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3350375"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1077)
                     /locus_tag="VGB88_02460"
     CDS             complement(133..1077)
                     /locus_tag="VGB88_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX9646332.1"
                     /translation="MTAAGARTADLANAVAAWRRWLADEKRAAAHTLAAYGRDLDAFL
                     SFLADHLGGAPALADLERLSAADFRAWLARRAMAGSARSSTARALSVVRGFFRFLDRR
                     GLARNAALKGLRTPKVPRSVPRPLTPADAKAVLREVGQVAEDAWVAARDVALLTVLYG
                     CGLRIGEALALDLGDVAARPEAIVVTGKGAKQRLVPVLPAVWRAIDDYLALRPHALLA
                     VSPLFVGVRGGRLNAAVAQRQMRAARGALGLGDTATPHALRHSFATHLLGASGELRTI
                     QELLGHASLSTTQRYTEVAPEQMLEVYRRAHPRARARD"
     gene            complement(1085..1765)
                     /gene="fsa"
                     /locus_tag="VGB88_02465"
     CDS             complement(1085..1765)
                     /gene="fsa"
                     /locus_tag="VGB88_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX9646333.1"
                     /translation="MKFFVDTADIDEIRDLAATGLLDGVTTNPSLISKTARPFADVIA
                     DICAAVSGPVSAEVTATDTDGMVAEGRRLAEIAGNVTVKVPLTWDGLKACRRLADQGV
                     MVNVTLCFAPAQALLAAKAGATFVSPFIGRLDDLGQDGMALVADICQIFRQYPQTLTT
                     EVLVASIRSPRHVVDAAKLGAHVATIPPKVLRQLVQHPMTDKGLKAFLDDWRKTGQTI
                     LEQRQAAE"
     gene            1829..>2635
                     /locus_tag="VGB88_02470"
     CDS             1829..>2635
                     /locus_tag="VGB88_02470"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX9646334.1"
                     /translation="MTPAPASGGGVAVLLPLPLAGTYDYLVGDRPAPPPGSFVRVPLG
                     PRRATGVVWGAAGGDVAPQRLRAIEAVLEVPAMGEALRRFIDWVAGYNLAAPGSVLRM
                     AMSVPAALAPPRPVELLVRGAATPDRLTARRHAVLQAAARRGPLTRAALMRETGAGAG
                     VVQGLVKCGALGVVARTAETDWPAPDPSRPGHVLSHAQAEAARALVAEVAERRFAPTL
                     LDGVTGAGKTETYLEAVAAALAAGRQVLVLLPEIALGAQWLARFETRFGAP"
BASE COUNT          315 a          975 c         1008 g          337 t
ORIGIN      
        1 cgtcgtcacc agcgcgtccg agcgcaacct ccggcaggag ctcgaggtca cactcaccat
       61 tcacaacttc accgcgatac gccgcgccgc gcgcgccggc gtgctcgcca ggcgcctcgc
      121 ccccaccgtg cgctagtcgc gcgcgcgggc gcgggggtgg gcgcgacggt agacctcgag
      181 catctgctcg ggcgcgacct cggtgtagcg ctgggtggtc gagaggctgg cgtggccgag
      241 cagctcctgg atggtgcgca gctcgccgct cgcgcccagc aagtgggtgg cgaagctgtg
      301 gcggagcgcg tgcggggtcg cggtgtcgcc gaggccgagg gcgccgcgcg cggcgcgcat
      361 ctggcgctgg gcgaccgccg cattgagccg gccgccgcgc acgccgacga acaagggcga
      421 gacggcaagg agcgcgtgcg gtcgcagcgc caggtagtcg tcgatcgccc gccacaccgc
      481 cggcagcacc ggcaccaggc gctgcttggc gcccttgccg gtgaccacga tcgcctcggg
      541 gcgcgccgcg acgtcgccca agtcgagcgc cagcgcctct ccgatgcgca gcccgcagcc
      601 gtacaggacc gtcaagagcg ccacgtcgcg cgccgccacc caggcgtcct cggcgacctg
      661 gccgacctcg cgcagcaccg ccttggcgtc ggcgggggtg agggggcgcg gcaccgagcg
      721 cggcaccttg ggcgtgcgca agcccttgag cgccgcattg cgggcgagcc cgcgccggtc
      781 gaggaagcgg aagaacccgc gcacgacgct gagcgcgcgc gcggtcgagc tgcgcgcgct
      841 gcccgccatc gcgcgccggg cgagccaggc gcggaagtcg gccgcactca gccgctcgag
      901 gtcggcgagc gccggcgcac cccccagatg gtcggcgagg aagctgagga acgcgtcgag
      961 gtcacggccg tacgcggcca aggtatgcgc cgcggcacgc ttctcgtcgg cgagccagcg
     1021 ccgccaggcc gcgaccgcgt tcgcgaggtc ggcggtgcgc gcgccggccg cggtcacggc
     1081 cgcatcactc ggccgcctgg cgctgctcca ggatggtctg cccggtcttg cgccagtcgt
     1141 cgaggaacgc cttgaggccc ttgtcggtca tcgggtgctg gacgagctgg cgcagcacct
     1201 tgggcgggat ggtggcgacg tgggcgccga gcttggccgc atcgaccacg tggcgcgggc
     1261 tgcggatcga ggcgaccagg acctcggtgg tcagggtctg cggatactgc ctgaagatct
     1321 ggcagatgtc ggcgaccagc gccatgccgt cctggccgag gtcgtcgagg cggccgatga
     1381 acggcgacac gaaggtcgcc cccgccttgg ccgcgagcag cgcctgcgcc ggcgcgaagc
     1441 agagcgtgac gttgaccatc acgccttggt ccgcgagccg ccggcaggcc ttgaggccgt
     1501 cccaggtgag cggcaccttg accgtgacgt tgccggcgat ctcggcgagc ctgcggccct
     1561 cggcgaccat gccgtcggtg tcggtggcgg tgacctcggc gctgaccggc ccgctgaccg
     1621 ccgcgcagat gtccgcgatc acgtccgcga acgggcgtgc ggtctttgat ataagggacg
     1681 ggttggtggt caccccgtcg agcagcccgg tcgccgccag gtcgcgaatc tcgtcaatgt
     1741 cggcggtatc gacgaagaac ttcatggccc tcgctgcctc cgcttgcaga gcgcccgacg
     1801 cgcgcgcgca acgctccgag tctagccgat gacgccggcc cccgccagcg gcggcggcgt
     1861 cgcggtgctg ctgccgctgc cgctcgccgg cacttacgac tatctggtcg gcgaccggcc
     1921 ggccccgccg cccggcagct tcgtgcgggt gccgctcggg ccccgccggg cgaccggggt
     1981 ggtctggggc gcggccggcg gcgatgtcgc gccccagcgc ctgcgcgcga tcgaggcggt
     2041 gctggaagtg ccggcgatgg gtgaggcgct gcgccggttc atcgattggg tggccggcta
     2101 caatctcgcc gcgcccggct cggtgctgcg catggcgatg agcgtgccgg cggcgctcgc
     2161 cccgccgcgc ccggtcgagc ttctggtgcg cggcgccgca acccccgatc ggctcaccgc
     2221 gcgccggcat gccgtgctcc aggcggcggc gcggcgcggg ccgctgacgc gcgccgcgct
     2281 gatgcgcgag accggcgccg gcgcgggcgt ggtgcagggc ctggtcaaat gcggcgcgct
     2341 cggcgtggtc gcgcgcacgg ccgagaccga ctggccggcg cctgatccct cgcgcccggg
     2401 ccacgtgctg tcacacgccc aggccgaggc cgcgcgcgcg ctggtcgccg aggtcgcaga
     2461 gcgccgcttc gcgcccaccc tgctcgacgg cgtcaccggc gccggcaaga ccgagaccta
     2521 tctcgaggcg gtggccgccg cgctcgccgc cggccgccag gtgctggtgc tgctgcccga
     2581 gatcgcgctc ggcgcccagt ggctcgcccg cttcgagacc cgcttcggcg cgccg
//
LOCUS       DASWHU010000067         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_395329, whole genome shotgun sequence.
ACCESSION   DASWHU010000067 DASWHU010000000
VERSION     DASWHU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395329"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1529)
                     /locus_tag="VGB88_02475"
     CDS             complement(<1..>1529)
                     /locus_tag="VGB88_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX9646335.1"
                     /translation="GASGTKTATATAAADTGNAHILALRPAVTVIGLVSATLVCGTPN
                     QVEVLFSAPVTAATAQNIANYALDGGLAITSAVLGTDARIVTLTTSPDLQSQLYTLTV
                     NNVAGAGGGMVAPNSQTTFFAEGGFLSGLRGTYYDQNGVQRAYFTGNTVVRVDGPLDF
                     DWGAGAPVAGIGADDFSVVWEGFVTPTVTGSYTFRTRSDDGIRLYIDGNLVIDNWTDH
                     GPTNDDSAPIALTADQRYSVRMEFYERGGGAVAQLSWSGPATGGFQFIPRSNLSHFCG
                     LPRPVAFYKMDEAGWAGAGDVADSSGNALHGTALGGAIPVPARVCNGAQLDGVARYLE
                     VADDNRLDMAGSFTVTAWINANALGAELKTIVSKDENFEFHLDNTGQINWWWQDSGGT
                     AQTFTTTGTAIVPGNWVHVAVVYAPGSQVIYIDGVSRGTATFAGGLLNNADPLQIGAD
                     QYPGQPQRVFDGLIDEVRVYGQALTQADIALIIADTRPCAVTLSHYEVAYPNGATGVT
                     C"
BASE COUNT          208 a          502 c          568 g          251 t
ORIGIN      
        1 cgcaggtgac gccggtcgcg ccgttcgggt aggcgacctc gtagtgcgag agcgtgaccg
       61 cgcaggggcg cgtgtcggcg atgatcagcg cgatgtcggc ttgcgtgagc gcctggccgt
      121 agacgcgcac ctcgtcgatc aggccgtcga acacgcgctg cggctggccc gggtactggt
      181 cggcgccgat ctgcagcggg tcggcgttgt tgagcagccc gccggcgaaa gtcgccgtgc
      241 cgcgcgacac gccgtcgatg tagatgacct ggctgcccgg tgcgtagacc acggcgacgt
      301 ggacccagtt gccgggaacg atggcggtgc cggtcgtggt gaaggtttgc gcggtgcccc
      361 cgctgtcctg ccaccaccag ttgatctggc cggtgttgtc gaggtggaat tcgaaattct
      421 cgtccttgga aacgatggtc ttcagctccg cgccgagcgc gttggcgttg atccaggcgg
      481 tgacggtgaa gctgccggcc atgtcgagcc gattgtcgtc ggcgacctcc aggtagcggg
      541 cgacgccgtc cagctgcgcg ccgttgcaca cccgggcggg caccgggatc gcgccgccta
      601 acgcggtgcc atgcagcgcg ttgccgctcg agtcggcgac gtcgccggcg ccggcccagc
      661 ccgcctcgtc catcttgtag aaggccacgg gccgcggcag gccgcagaaa tgcgacaggt
      721 tcgagcgcgg gatgaactgg aagccgccgg tggcggggcc gctccaggag agctgcgcca
      781 ccgcgccgcc gccgcgctcg tagaactcca tgcgcacgct gtagcgctgg tcggcggtga
      841 gcgcgatcgg cgcgctgtcg tcgttggtcg gcccgtgatc ggtccagttg tcgatcacca
      901 ggttgccgtc gatgtagagc cggatcccgt cgtcgcttct ggtgcggaag gtgtagctgc
      961 cggtcaccgt gggcgtgacg aagccttccc acacgacgct gaagtcgtcc gcgccgatgc
     1021 cggccaccgg ggcgccggcg ccccagtcga agtcgagcgg accgtctacg cgcaccacgg
     1081 tattgcccgt gaaataggcg cgctgcacgc cgttctggtc gtagtaggtg ccgcgcagcc
     1141 ccgagagaaa gccgccctcg gcgaagaacg tggtctgcga gttgggcgcg accatgccgc
     1201 cgcccgcgcc cgcgacgttg ttcacggtca gcgtgtagag ctggctctgc aggtcggggc
     1261 tggtggtgag cgtgacgatc ctcgcgtcgg tgccgagcac cgccgaggtg atcgcgaggc
     1321 cgccgtcgag cgcgtaattg gcgatgttct gcgccgtcgc cgccgtgacc ggcgccgaga
     1381 acaggacttc gacctggttc ggcgtcccgc agacgagggt ggcggagacc aggccgatca
     1441 cggtgactgc cggacgcagc gcgaggatgt gcgcgttgcc cgtatcggcc gccgccgtcg
     1501 cggtcgcggt cttcgtgccg ctcgcgccc
//
LOCUS       DASWHU010000068         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2986908, whole genome shotgun sequence.
ACCESSION   DASWHU010000068 DASWHU010000000
VERSION     DASWHU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986908"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VGB88_02480"
     CDS             <1..1007
                     /locus_tag="VGB88_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9646336.1"
                     /translation="DRLPARAPALYLIDIQRDQAEAVAALIAATPGARLIEQVPMLRG
                     RITRLDGVPVAEAEIDPGGRWLVRGDRGVTFAALPPERAPIVAGRWWPADYAGPPLVS
                     LDADSARDLHLDVGGRIALSVLGRTIEAEVAALRRIDWDDFGINFVVVLSPGVLDAAP
                     YTLIASIAADPAAEAALVSALGADLANVSAIPVRGILARIRAAIDAVAAALAATAGVA
                     LAAGVLVLTGVIAAARQARIYEAVVLEVLGASRRQLLAAYLVEHALLGAAAGTVALAA
                     GTLAAWAFVTQLAGEAWAFDAARALAVVLGCIAITTALGFAATWRALGQKPIQVLRAP
                     "
     gene            1116..1835
                     /locus_tag="VGB88_02485"
     CDS             1116..1835
                     /locus_tag="VGB88_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9646337.1"
                     /translation="MAFERRTVMGRAGAATTVAIDEGLRAFMLKVYNYMGLGLAVTGA
                     VALFTASSETMMQAIYGSPLAWLVMLSPLAFVLVLSFGINRLSVGATQALFWAFAVVM
                     GLSMSTIFVVYTGTSIARVFFITAATFAAMSLYGYTTRRSLASMGAFLFMGLIGLIIA
                     MVVNLFLASSALQFAVSIIGVLIFTGLTAWDTQRLKLMYVAGEGGTAVAEKKAIYGAL
                     SLYLDFINLFYMLLSLLGNRQ"
     gene            1972..2244
                     /locus_tag="VGB88_02490"
     CDS             1972..2244
                     /locus_tag="VGB88_02490"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9646338.1"
                     /translation="MAVKETSAPREFAFTAVFEPAEEGGYVVSFPGIRDLMTEGETLE
                     EARRMAADCLRCYLEALEKEGAEFPQSESAELQPIVEKVSIRLKAG"
     gene            2241..2471
                     /locus_tag="VGB88_02495"
     CDS             2241..2471
                     /locus_tag="VGB88_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9646339.1"
                     /translation="MTGRLPALRPRQVIAALERSGFFVHHATGSHYALRHPDNPQLRV
                     TVPYHNRDLKRAVLRAILRQAGLSPQAFLEHL"
     gene            complement(2740..3705)
                     /locus_tag="VGB88_02500"
     CDS             complement(2740..3705)
                     /locus_tag="VGB88_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646340.1"
                     /translation="MRGARAWLAAAVIAAVPALAHAQQEAPRYRSWTAGPAGGTQDMV
                     TQLRALVQEAERANAADPRFLTDLEALADAYDESAPVELLHDDFRDGDYTEGVAWTVT
                     RGRWWVEPAVGLRSLVAAPAASPAPTTQQQPYRRPTGEDLAAAVLGNLLGQVTGPQQQ
                     QSQQTQQTTTQTGQAAGADATPAEIQVASAIPNAFSARFEITSRARAGEFRAGVYQRS
                     LRDGWGYQIAYAPGAAQGLRLMAVSRGQATVIGTHDGVLNLEDGRLHVIDWSRSLAGE
                     TAVGVDGKEMIRVTDARLRDPFEGLGIANLGGDFALRDVSVKRGR"
BASE COUNT          564 a         1459 c         1430 g          651 t
ORIGIN      
        1 gcgaccggct gcccgcgcgc gccccggcgc tgtacctgat cgacatccag cgcgaccagg
       61 ccgaggcggt ggccgcgctg atcgccgcga cccccggcgc gcgcctgatc gagcaggtgc
      121 cgatgctgcg cgggcggatc acccgcctcg acggggtgcc ggtggccgag gccgagatcg
      181 acccgggcgg ccgctggctg gtgcgcggcg accgcggagt caccttcgcg gcgctcccgc
      241 ccgagcgcgc gccgatcgtc gccggccgct ggtggcccgc cgactacgcc ggcccgccgc
      301 tggtctcgct cgacgccgac agcgcgcgcg acctccacct cgacgtcggc ggccgcatcg
      361 cgctgtcggt gctcgggcgg accatcgagg ccgaggtcgc ggcgctcagg cgaatcgact
      421 gggacgactt cggcatcaac ttcgtggttg tgctgtcgcc gggcgtgctc gacgcggccc
      481 cctacaccct gatcgcgagc atcgcggccg atcccgccgc cgaggcggcg ctggtgagcg
      541 cactcggcgc ggacttggcc aacgtctcgg cgatcccggt gcgcgggatc ctggcgcgca
      601 tccgcgccgc gatcgacgcg gtcgcggcgg cgctggcggc gaccgcgggc gtcgcactcg
      661 ccgccggcgt gctggtgctc accggcgtga tcgccgccgc ccgccaggcg cgaatttacg
      721 aggcggtggt gctcgaggtg ctcggcgcca gccgccgcca gctgcttgcc gcctacctgg
      781 tcgagcacgc gctgctcggc gcggcggcgg gcacggtggc gctcgccgcc ggcacgctcg
      841 ccgcctgggc gttcgtcacc cagctcgccg gggaggcgtg ggcgttcgac gcagcgcgcg
      901 cgctcgcggt cgtgctcggc tgtatcgcca tcaccaccgc gctcggcttc gccgccacct
      961 ggcgcgcgct cgggcagaag ccgatccagg tgctgcgcgc gccgtaggcc gtgcgccggt
     1021 gcccgaacga ggttaggatg gcgcgttgat tcgcgcgccc gaaatcccat ataggatgcg
     1081 ggtctggaac cattctcagg tcgaaaggac acgacatggc attcgagcgc agaaccgtga
     1141 tggggcgcgc cggcgcagcg accacggtcg cgatcgacga aggcctgcgc gccttcatgc
     1201 tcaaggtcta caactacatg ggcctgggcc ttgcggtgac cggcgcggtg gcgctgttca
     1261 ccgcctcgtc cgagaccatg atgcaggcga tctacggctc gccgctggcg tggctggtga
     1321 tgctgtcgcc gctcgccttc gtcctggtgc tgagcttcgg catcaaccgg ctgagcgtgg
     1381 gcgcgacgca ggcgctgttc tgggcgttcg cggtggtcat ggggctgtcg atgtcgacca
     1441 tcttcgtggt ctacaccggc acctcgatcg cgcgcgtgtt cttcatcacc gccgccacct
     1501 tcgccgcgat gagcctgtac ggctacacca ccaggcgcag cctggccagc atgggcgcgt
     1561 tcctgttcat gggcctgatc gggctgatca tcgcgatggt ggtcaacctg ttcctcgcct
     1621 cgagcgcgct ccagttcgcg gtctcgatca tcggcgtgct gatcttcacc gggctgaccg
     1681 cgtgggacac ccagcggctc aagctgatgt acgtcgccgg cgagggcggc accgcggtgg
     1741 ccgagaagaa ggcgatctac ggcgcgctgt cgctctatct cgacttcatc aacctgttct
     1801 acatgctgct gtcgctgctc ggcaaccgcc agtagcgcac cgccgcgcag gagcccatct
     1861 ccggaccccc ggcggcaccg tccgccgggg gttttgcgtc cttaaccctc gcgggcgatg
     1921 cctgtggtat aaggcgggac ctcgtcacac accgagtcaa ttgtgggcag gatggccgtc
     1981 aaggaaacca gcgcgcctcg ggaattcgcc tttaccgcgg tcttcgagcc ggccgaggaa
     2041 ggcggctatg tggtgtcgtt tccgggcatc cgggacctca tgaccgaggg cgagacgttg
     2101 gaggaggcgc gccgaatggc cgccgattgc ctgcgctgct atctcgaagc gctggagaag
     2161 gaaggcgcgg agttcccgca gagcgagtcc gcggagcttc agccgatcgt cgaaaaggtc
     2221 agcatccggc tcaaggccgg atgaccggcc ggctgccggc gctcaggccg cggcaggtta
     2281 ttgccgcact cgagcgcagc ggcttcttcg tgcaccacgc caccggcagc cattatgccc
     2341 tacggcaccc agacaacccg cagctcaggg tcaccgtccc gtatcacaac cgtgacctca
     2401 aacgggccgt ccttcgcgcc atcctgcgcc aggccgggct gtcgcctcag gccttccttg
     2461 aacaccttta gtcgtgtgtg acgagcaagc ccctattcag cacgccttcg cgctcaatgc
     2521 gctcattgta ggcctcgatc gcctcggcgt tgcgccgcag ccactccctc gccccggcct
     2581 ctcgtgccgt tgcgaccagc gcgctcagta ctgtttcgga aaggccggtg tgacgcgcca
     2641 tcgcgtcagc ggcgacgtcg aaatcgacgg gggcgtccgt gattcggcgg caaccgcctt
     2701 cgattgcgcg cgaacgtctt ctggaccgcc ccgccggcat taccgcccgc gcttgaccga
     2761 gacgtcgcgg agcgcgaaat cgccgccgag gttggcgatg ccgaggccct cgaacgggtc
     2821 gcgcaggcgc gcatcggtga cccggatcat ctcctttccg tcgacgccga ccgcggtctc
     2881 gcccgccagc gagcggctcc agtcgatcac gtgcaggcgc ccgtcctcga ggttgagcac
     2941 gccgtcgtgg gtgccgatca cggtggcctg gccgcgtgag acggccatca gccgaagccc
     3001 ctgcgccgca cccggcgcat aggcgatctg gtagccccag ccgtcgcgca gcgagcgctg
     3061 gtagacgccc gcgcggaact cgccggcgcg tgcccgcgag gtgatctcga agcgtgccga
     3121 gaaggcgttc gggatcgcgc tcgcgacctg gatctcggcc ggggtggcat cggcgccggc
     3181 cgcctggccg gtctgggtgg tggtctgctg ggtctgttgg ctctgttgct gctgcgggcc
     3241 ggtcacctgg ccgagcaggt tgccgagcac ggcggcggcg aggtcctcgc cggtcgggcg
     3301 ccggtacggt tgctgctggg tggtcggggc cgggctcgcc gccggcgccg cgaccagcga
     3361 gcgcaagccg accgcgggct cgacccacca gcgcccgcgg gtcactgtcc aggcgacgcc
     3421 ctcggtgtag tcgccgtcgc ggaagtcgtc gtgcagcagc tcgaccggcg cgctctcgtc
     3481 ataggcgtcg gcgagcgcct cgaggtcggt gaggaagcgc gggtcggcgg cattcgcgcg
     3541 ctctgcctcc tggaccagcg cacgcagctg cgtgaccatg tcctgggtgc cgccggcggg
     3601 gcccgcggtc cacgaccggt agcgcggcgc ctcctgctgg gcgtgcgcaa gcgccggcac
     3661 cgccgcgatc accgccgcgg ccagccacgc tcgcgcgcct cgcaccccgc tccccgtcat
     3721 cgcccgcgag actaccacgc cgcgcccgcc gcggaagtca caatgcggac cgccgacgct
     3781 ggatcgtggc gcaggtcgag atgaatatca acttcttgcg caaccaaaac acctaggatt
     3841 ccgaccagtg acatgacagc tgaggcaaag gctggcgccc cgaccaatgc catcgcgcgc
     3901 aagatcgacg cgctccagcg gaagggtgcg atgcggtcgg tggatatcgc caacctgctg
     3961 ggagcgggcg cgtggcctgc tgggagaata gcgccgcgtc ggcgcgctgt gctcggcgac
     4021 ctctcgattg cataatgccg cgatgcggct aaatttaacc acatggcaca gaaggaaacc
     4081 gttgccgcca gggagttgac ctac
//
LOCUS       DASWHU010000069         8102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2987645, whole genome shotgun sequence.
ACCESSION   DASWHU010000069 DASWHU010000000
VERSION     DASWHU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987645"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1719)
                     /locus_tag="VGB88_02505"
     CDS             complement(379..1719)
                     /locus_tag="VGB88_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9646341.1"
                     /translation="MTARRPASLFGRATAVLVGGLVLTHLISLAIHYGDESHRAALTG
                     EDALVTRIASAARALASIPAEQRAAMARELSDPGVAVGWPGPDRPAAEAAAGDAHAAR
                     VRDRLAAALPAGFAVGRAAILACSPTELCGEAGERLEAVVGLPDGSTLSIVTGLGGFG
                     AVTMRRIALEILIMAAGIVALSLWAAGWLTRPLSTFALAADRLGRDLRAPPIAQAGPR
                     EIRQLARAFNGMQSRLRRFLDDRTQMLAAVSHDLRTPITRLRLRAEFIDDQDAHARML
                     ADLDEMERMIDSALAFARDEAGGETRRRVDLTALVESICHDAEDAGQRVSCTGSAPAP
                     VEGNRLALKRAIANLIDNAVKHGGAADVALGGDDGEARITVDDRGPGLPDRELEEVFR
                     PFYRAAGRGGDAAGSGLGLAIARSILRGHGGEVRLENRPGGGLRATANLPCPRS"
     gene            complement(1716..2435)
                     /locus_tag="VGB88_02510"
     CDS             complement(1716..2435)
                     /locus_tag="VGB88_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9646342.1"
                     /translation="MSRPEHLLVVDDDPEIRDLLARYLARNGYRVSVAEDADSLRRAL
                     AADRVDLVILDIMLPGRDGLDITRDLRASSAIPIIMLTARGEDTDRIVGLEIGADDYL
                     AKPFNPRELLARIKTVLRRVSAAPARADAPRAKRLRFAGWTLDLAARELSSADGVEVA
                     LTAGEFDLLAAFAEHPQHVLSRDRLLDLTRGRLAGPFDRSIDVQVGRLRRRIEADPRE
                     PKLVKTVRGGGYMFAVQVERE"
     gene            complement(2592..3212)
                     /locus_tag="VGB88_02515"
     CDS             complement(2592..3212)
                     /locus_tag="VGB88_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646343.1"
                     /translation="MMGPGMMGPGMMGGGMMGPGMMGPGMMGPGMMGGGMMGGHGMMG
                     GRGMGGMMGQGGAPAVVINIYPGGGMGMMGPGMGMMGPGMGGMMGPGMGMMGTPQGED
                     EPGQGSAVPGMGMMGSGMMGPGMMGGGMGMMGAPGDLAESGLDLSAADVRARLEGWLG
                     ERSGLSVGEVVEIDDDLIGAQLLDADGKPVRRLVVARHTGASMAVR"
     gene            complement(3567..4292)
                     /locus_tag="VGB88_02520"
     CDS             complement(3567..4292)
                     /locus_tag="VGB88_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX9646344.1"
                     /translation="MSIRAVIRFCAVAISLAAAPVFADEEGEHEDEGGMHAGHWMAPA
                     EAQQRRNPVPVTPEALAEAAEVYAETCSICHGPEGKGDGDAAEGLDPKPADFSRMVPI
                     HPDGDLAWKIEHGRGTMPAWGEMLDEVTIWQLVNYLRHEIAATTASAETGAPATATRA
                     TVNLSHEAITALGWPAMTMDMALLGGADARGLEPGQEVDFELARGPDGVYGVSAIWPA
                     GKAPPPGADAVRGHGTLNAIAGE"
     gene            complement(4321..5679)
                     /locus_tag="VGB88_02525"
     CDS             complement(4321..5679)
                     /locus_tag="VGB88_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX9646345.1"
                     /translation="MLTRRGLFTTGGVLAGGAVAAAVPRQTLAAAGPGGGVWDKSYSG
                     GPVDRPALSPGLPGRDYMPVVTPNGGPLPVKVVDGVKVFHLVAEEIEHEFAPGLVATC
                     WGYGGRVNATTIEAVEGDRVRIYLTNRLSVPTTVHWHGIYLPNGMDGVSGLSQRPIRP
                     GETFRYEWTLRQSGTFMYHSHHDTMTQEAMGLIGMLVIHPRDPAPEQRVDRDFALLLS
                     EWFIDPGTHRPNPFEMTDFNVLTINGRCFPGTAPLVVRTGDRVRIRFGNLSATDHHPI
                     HLHGYRFRVAATDGERIPLSAQWSETTVLVAVGQTREIEFVADAPGDWAMHCHMTHHV
                     MNQMGHAIPNMVGIDPGDLDRSVGSLLPGYMTMGQGGMSGMGGMPMAVPENSIPMVGA
                     GGPFDYIDMGGMFTVLKVRDDLASYTEDPGWYDHPPGTVADLAGPEELARDGVDPGAK
                     PA"
     gene            complement(5690..7117)
                     /locus_tag="VGB88_02530"
     CDS             complement(5690..7117)
                     /locus_tag="VGB88_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9646346.1"
                     /translation="MGVCARILVSVMAAGAVAGCAAVTEEAGFGDVRQTVAERTGEDI
                     RWRQGTAEDRALDEAVAKLLADGLTADEAVQVALLNNRRLQATYQDLGVAQAALVQAG
                     LLANPIFDAAVMFPLTGGAPDLELSVVQDFLGIFYLPLRTRVAESEFDATKHRVAGAV
                     IDLAGRTRAAFYRAQADVQQLELREQVTRATAVSLEAMQRLRAAGNVTRLDVDNEQAL
                     HERARLDLAAGEAGVLASRERLNRLMGLWGAETEWAVAPRLPDLPDTETDLETVERRA
                     VEASLDLAAMRSELEAFGRQLGLTEASALLPFADAGAAAERDDRDWNVGPVLALPIPL
                     FDLGQARVATAQAEFRRRHQRYWALAVEIRSATRAAREDLRAARDMARHYRDVMLPLS
                     ERIVEETQKRYNAMQLGVFQLLLAKQQQIATGAAYIEALRAYWVARAELDQILQGRLS
                     DMAPEIDARAGASMSPGMARPGADG"
     gene            complement(7181..7441)
                     /locus_tag="VGB88_02535"
     CDS             complement(7181..7441)
                     /locus_tag="VGB88_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646347.1"
                     /translation="MSRKLLTIVTVMLLVSACGTGAVLPPPGPDHPASPDAAVAPLPS
                     RMSALDGDAAGLFEGEGDAMAHDRPADAGGAMEGQHSMESME"
     gene            complement(7793..>8102)
                     /locus_tag="VGB88_02540"
     CDS             complement(7793..>8102)
                     /locus_tag="VGB88_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9646348.1"
                     /translation="VDGHLAFLRTELAITPAQEPLWNDFAATIRGNAEQMRTTHHWEH
                     IYGAGTLPERLARYEEHLSARLDMLHKAQTAVSPLYAALDEAQKARADQILFGHMEHM
                     "
BASE COUNT         1133 a         2960 c         2783 g         1226 t
ORIGIN      
        1 cgtcggtatc gacgcccgcg cccagatagg cggtatccac cggcagcgcc cggctcatct
       61 tcgctcctcg gcccgtctcc gcgaccggcc cgtcaaatgc cgactccccg gccccgtatg
      121 tggcccgcca cccgaagccg aaagcgtagg gtggcggccg gcgggtccat acgaattctc
      181 gccggccgtg tcaccggcac gtcgcgatac cgagcgcgta tatcagggaa cccttagtcc
      241 ccgtgccgcg ggctcgcaag gagaagctga agcgagccag gccccgtcgc cccggggcac
      301 cgctcccatc cgaggcgact cgaaagtctg tcggcggttg tcctcggcag ggcgcacgag
      361 ccatccatcg ggcgcgactt aagacctagg gcacggcagg ttggcggtcg cccgcaggcc
      421 gccgccgggg cggttctcca gcctgacctc gccgccatgt ccgcgcagga tcgagcgtgc
      481 gatcgccagg ccgagcccgg accccgcggc gtcgccgccg cgaccggcgg cgcggtagaa
      541 cggccggaac acctcctcga gctcgcggtc cggcagaccc ggcccgcggt cgtcaaccgt
      601 gatccgggcc tcgccgtcgt cgccgccgag ggcgacatcg gcggcgccgc cgtgcttgac
      661 cgcgttgtcg atcaggttcg cgatcgcgcg cttgagcgca agccggttgc cctcgaccgg
      721 cgccggtgcg ctgccggtgc agctcacccg ctggccggcg tcctcggcgt cgtggcagat
      781 gctctcgacc agggcggtga ggtcgacccg cctgcgggtc tcgccgccgg cctcgtcgcg
      841 ggcgaaggcg agcgcgctgt cgatcatccg ctccatctcg tcgaggtcgg cgagcatgcg
      901 cgcatgcgcg tcctggtcgt cgatgaactc ggcgcgcagg cgcaggcgcg tgatcggggt
      961 cctcaagtcg tgcgagaccg cggccagcat ctgggtgcgg tcgtcgagga accggcgcag
     1021 gcggctctgc atgccgttga acgcgcgcgc gagctggcga atctcgcgcg gcccggcctg
     1081 cgcgatcggc ggcgcgcgca ggtcgcgccc gagccggtcg gcggcaaggg cgaaggtgct
     1141 gagcgggcgg gtcagccagc ccgccgccca cagcgacagg gcgacgatgc cggcggccat
     1201 gatcaggatc tcgagcgcga tccgccgcat cgtcaccgcg ccgaagccgc ccaggccggt
     1261 gacgatcgac agcgtcgagc cgtcgggcag cccgaccacg gcctcaagcc gctcgccggc
     1321 ctcgccgcaa agttcagtgg gactgcacgc gaggatcgcc gcgcggccga cggcgaagcc
     1381 cgcgggcagc gcggcggcca gccggtcgcg cacccgcgcc gcgtgcgcgt cgccggccgc
     1441 cgcctccgcc gccggccggt cggggcccgg ccaaccgact gcgacgccgg ggtccgacag
     1501 ctcccgcgcc atggccgcac gctgctccgc agggatcgac gccagcgccc gcgccgccga
     1561 ggcgatgcgc gtcaccagcg cgtcctcgcc ggtcagcgcg gcgcggtgcg actcgtcgcc
     1621 atagtggatc gcgaggctga tcagatgggt caggaccagg ccgccgacca gcaccgcggt
     1681 cgcccgtccg aacaggctgg cgggccggcg ggcggtcatt cacgctcgac ctggaccgca
     1741 aacatgtagc cgccgccgcg caccgtcttg accagcttcg gctccctggg gtcggcctcg
     1801 atccgccgcc gcaaccgccc gacctggacg tcgatgctgc ggtcgaacgg gccggcgagg
     1861 cggccgcgcg tcaagtcgag cagccggtcg cggctgagca cgtgctgcgg atgctcggcg
     1921 aacgccgcca gcaggtcgaa ctcgcccgcg gtgagcgcga cctcgacgcc gtccgcggag
     1981 ctcagctcgc gcgccgccag atccagcgtc cagccggcga agcgcaagcg cttcgcgcgc
     2041 ggcgcgtccg cgcgcgccgg ggcggcgctc acccgcctca gcacggtctt gatccgcgcc
     2101 agcagctcgc gcgggttgaa cggcttggcc agatagtcgt cggcgccgat ctcgaggccg
     2161 acgatgcgat cggtatcctc gccgcgcgcg gtcagcatga tgatcggtat cgcggagctt
     2221 gcgcgcaggt cgcgggtgat gtcgaggccg tcacgcccgg gcagcatgat gtcgaggatg
     2281 accaggtcga cccggtccgc cgcgagcgcg cggcgcaggc tgtcggcgtc ctcggcgacg
     2341 ctcacccgat agccgttgcg ggcgagatag cgggcgagga gatcgcgaat ctccgggtcg
     2401 tcgtcgacca ccagcaaatg ctcgggcctg ctcatggcgc aacatggacc gttcgggcgc
     2461 gtggggccgg tcgttggtgt catagcccat cgaccggccg cgcgccaagc ggcggctaaa
     2521 gaccgtcaca tgccgctaaa gggacaccgg cacacccctc gccgtgccgc cgccacagga
     2581 gctgcgccga ctcagcgcac cgccatgctg gcgccggtat gacgggcgac gaccaggcgg
     2641 cggaccggct tgccgtcggc gtcgaggagc tgggcgccga tcaggtcgtc gtcgatctcg
     2701 accacctcgc cgaccgacag gccgctgcgc tcgccgagcc agccttccag gcgggcgcgc
     2761 acgtcggccg ccgacagatc caggccgctc tccgcgagat cgcccggcgc tcccatcatg
     2821 cccattccgc cgcccatcat gccggggccc atcatgccgg agcccatcat gcccatgcct
     2881 ggcacggccg aaccctggcc cggctcatcc tcgccttgcg gcgtgcccat catgcccatg
     2941 ccggggccca tcatgccgcc catgccgggg cccatcatgc ccatgccggg tcccatcatt
     3001 cccatgccac cgccgggata gatgttgatc accaccgccg gcgcgccgcc ctggcccatc
     3061 atgccgccca tgccgcggcc gcccatcatg ccgtggccgc ccatcatgcc gccgcccatc
     3121 atgccagggc ccatcatgcc ggggcccatc atgccggggc ccatcatgcc gccgcccatc
     3181 atgccgggtc ccatcatgcc ggggcccatc atgcccatgc cgccgccggc cggggcctgc
     3241 tcaggcgctg cctgaggcgt cgcggctccg ggctccgatt cgccgtggtg ttcctcctgg
     3301 gcaagcgcaa gagccggagc aagcgccatt gcgacgccaa tcatcgctac cccaattaca
     3361 gtgtgcctca tgacaatctc ctattcgttg ttatctcgtt ttcctgcgac cggccaatgc
     3421 gcaatacgtg ctacgcccgc ggggcgtatc tccatagtca ggcccggctt tggcgcacgt
     3481 ccagctggtc ggcccagccc gctgcagtcc atccgatgcg cgccaccgca gccacaacct
     3541 ggatcaatca cacgggcagc cgtgacctac tcgcccgcga tagcgttcag ggtgccgtgc
     3601 ccgcgcaccg cgtcagcccc ggggggcggc gccttgccgg cgggccagat cgcgctcact
     3661 ccgtacaccc cgtcggggcc gcgggcgagc tcgaagtcga cctcttgtcc gggctcgagg
     3721 ccgcgcgcgt cggcaccgcc gagcagcgcc atgtccatgg tcatcgccgg ccagccgagg
     3781 gcggtaatcg cctcgtggct caggttcacc gtcgcccgcg tcgcagtcgc cggagccccg
     3841 gtttccgcgc tcgccgtcgt cgcggcgatc tcgtggcgca ggtagttgac cagctgccag
     3901 atggtcacct cgtccagcat ctcgccccag gcgggcatgg tgccgcggcc gtgctcgatc
     3961 ttccaggcga ggtcgccgtc cgggtggatg ggcaccatcc ggctgaagtc cgcgggcttc
     4021 ggatcgagac cttcggcggc gtcgccgtcg cccttgccct cgggcccgtg gcatatcgag
     4081 caggtctcgg cgtacacctc ggccgcctcg gcgagtgcct ccggggtcac cggcacgggg
     4141 ttgcggcgct gttgcgcctc ggccggcgcc atccagtggc cggcgtgcat cccgccctcg
     4201 tcctcgtgct cgccctcctc gtcggcgaac accggcgcag ctgccagcga gattgcgacc
     4261 gcgcaaaacc gaatgactgc gcgaatgctc atcggtgcga tccctcaatt cgacgacgca
     4321 tcacgccggc ttggcgccgg ggtcgacgcc gtcgcgtgca agctcctcgg ggccggcgag
     4381 atcggcgacg gttcccggcg gatggtcgta ccagccggga tcttcggtgt agctcgccag
     4441 gtcatcgcgc accttgagca cggtaaacat gccgcccatg tcgatgtagt cgaacgggcc
     4501 gccggcgccg accatgggga tgctgttctc cggaacggcc atcggcatcc cgcccatgcc
     4561 ggacattccg ccctggccca tggtcatgta gccgggcagc agcgagccca cgctgcggtc
     4621 gaggtcgccg ggatcgatgc cgaccatgtt cgggatcgcg tggcccatct ggttcatcac
     4681 gtggtgggtc atgtggcaat gcatcgccca gtcgcccggg gcgtcggcga cgaactcgat
     4741 ctcgcgcgtc tggccgacag cgaccagcac ggtggtctcc gaccattgag ccgatagtgg
     4801 gattcgctcg ccgtcggtgg cggcaacgcg gaatcgatag ccgtgcaaat ggatcggatg
     4861 atggtccgtg gcgctcaggt tgccgaagcg gatgcgcacc ctgtcgcccg tacgcaccac
     4921 cagcggcgcg gttcccggga agcaccggcc gttgatggtc agcacgttga agtcggtcat
     4981 ctcgaacggg ttcgggcggt gcgtgccggg gtcgatgaac cattcgctca gcagcagcgc
     5041 gaagtcgcga tcgacgcgct gctcgggcgc ggggtcgcgc gggtggatga ccaacatccc
     5101 gatcagcccc atggcctcct gggtcatggt gtcgtggtgc gagtggtaca tgaaggtgcc
     5161 cgactggcgc agcgtccact cgtacctgaa ggtctcgccg ggccggatcg ggcgctggct
     5221 gagtccgctc accccgtcca tgccgttagg cagatagatg ccgtgccaat ggaccgtggt
     5281 cggcacgctg aggcggttgg tgaggtagat gcgcacgcgg tcgccctcga ccgcctcgat
     5341 cgtggtcgcg ttcacccgcc cgccgtagcc ccagcacgtc gccaccagcc cgggcgcgaa
     5401 ctcgtgctcg atctcctcgg cgaccaggtg gaacaccttg acgccgtcga cgaccttcac
     5461 gggcagcggc ccgccgtttg gggtcaccac cggcatgtag tcccggcccg gcagcccagg
     5521 gctgagtgcc gggcgatcga ccgggccgcc gctgtagctc ttgtcccata cgcctccgcc
     5581 cggtcctgcc gcagccaagg tctgccgcgg cacggcggcg gcgacggcac ctccggcaag
     5641 cacgccgccg gtcgtgaaca agcctcgtcg cgtgagcatc tccgcctcct cagccgtcgg
     5701 cgccgggccg ggccatgccg ggtgacatcg atgcgcccgc gcgcgcatcg atctccggcg
     5761 ccatgtcgct cagtcgtccc tgaaggattt gatcgagctc ggcgcgcgcc acccaatagg
     5821 cccgcagcgc ctcgatatat gcagcgccgg tggcgatctg ctgctgcttt gccagcagca
     5881 gctggaaaac gcccaattgc atggcgttgt agcgcttctg cgtttcctcg acgatgcgct
     5941 cgctcaacgg caacatcacg tcgcgatagt gccgggccat gtcgcgcgct gcgcgcaggt
     6001 cctcgcgcgc cgcgcgcgtc gcggagcgga tttcaacggc gagcgcccaa tagcgttggt
     6061 ggcggcggcg gaactctgcc tgggcggtgg caacgcgcgc ctggccgagg tcgaacagcg
     6121 ggatcggcag cgcgagcacg gggccgacgt tccagtcgcg atcgtcgcgc tcggcggccg
     6181 cgccggcatc ggcaaagggg agcaatgcgc tcgcctcggt caggcccagc tggcggccga
     6241 atgcctcgag ctcgctgcgc atcgccgcga ggtcgaggct cgcctccacc gcccgccgct
     6301 ccaccgtctc gagatcggtc tcggtgtcgg gcaggtcggg aagccgcggc gcgaccgccc
     6361 actcggtctc tgcgccccac aggcccatca ggcggttgag ccgctcgcgg cttgccagaa
     6421 cgccggcctc gccggcggca aggtcgagcc gggcgcgctc gtgaagcgcc tgctcgttgt
     6481 cgacgtcgag ccgggtgacg ttgcccgccg cgcgcaaccg ctgcatcgcc tcgagcgaga
     6541 ccgccgtcgc gcgcgtcacc tgctcgcgca gctcgagctg ctggacatcg gcctgggcgc
     6601 ggtagaacgc ggcgcgtgtc cgcccggcga ggtcgatgac cgcgcccgcg acccgatgct
     6661 tggtcgcgtc gaactccgac tcggccaccc gcgtgcgcag cggcaggtag aagatgccga
     6721 ggaagtcctg gacgacgctg agctccaggt cgggagcgcc gccggtgagc gggaacatca
     6781 ccgcggcgtc gaagatcggg ttggcgagca gccccgcctg gaccagcgcc gcctgggcga
     6841 caccgaggtc ctgatacgtc gcctgcaggc gccggttgtt aaggagcgcg acctggaccg
     6901 cctcgtccgc ggtcaggccg tcggcaagca gtttcgcgac cgcctcgtcg agagcccggt
     6961 cttctgccgt cccctgacgc cagcggatgt cctcgccggt gcgctcggcg accgtctggc
     7021 ggacgtcgcc gaatcccgcc tcctcggtca ccgcggcgca gcccgcaacg gcgcctgccg
     7081 ccatcaccga gaccaggatg cgcgcacaaa cgcccatcgg acggctagct ggatttggcg
     7141 ccatagccgg cgttgttaat gcctcgccgg cgacaccgca tcattccatc gactccatcg
     7201 agtgctgtcc ctccatggcg ccgcccgcat cggcgggccg gtcgtgcgcc atcgcatcgc
     7261 cctcgccctc gaacagcccg gccgcgtcgc catccaaggc cgacatgcgg gacggcagcg
     7321 gcgcgacggc cgcgtcggga ctcgccgggt ggtcgggccc cggcggcggc agcacggccc
     7381 cggtgccgca ggccgagacc agaagcatta ccgtcacgat ggtcaacaac ttgcggctca
     7441 tcaacatccg ctcccgtggg cacagtttcc gatcgggctt attcttggca tcgttcggcg
     7501 cgatcgcata gcaaccgatt gtaaccgcac gatgagtgcc ggcccttgaa ccgcgaataa
     7561 tctgtaaccg aatgtaacgt tttgttacgc cggtgagtca gcgccacacg gtgccatgct
     7621 atgaaacgcc gctggcggtt tgcccggtct gcttgcgacc gggcttcgcc gagcggccgg
     7681 agttctgagc cggtaccgga ggtccgccgg gcaggcgcat cgatggctcg atatgcctgc
     7741 ccggcgcgtc gaagaggctc gttgcgagcc cgatctgttg gtccgatggc ccctacatgt
     7801 gctccatatg gccgaacagg atctggtcgg cacgcgcctt ctgggcttcg tcgagcgcgg
     7861 catagagcgg gctcactgcg gtttgtgcct tgtgcagcat gtcgaggcgc gccgacaggt
     7921 gctcttcgta ccgggcgagc ctctcgggca gggtgcccgc gccgtagatg tgctcccagt
     7981 gatgagtggt gcgcatctgc tcggcgttgc cgcggatggt ggcggcaaag tcgttccaca
     8041 gcggctcttg cgcgggcgtg atcgcgagct cggtgcgcag gaaggcgagg tggccgtcga
     8101 cg
//
LOCUS       DASWHU010000070        10405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2609586, whole genome shotgun sequence.
ACCESSION   DASWHU010000070 DASWHU010000000
VERSION     DASWHU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609586"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGB88_02545"
     CDS             <1..902
                     /locus_tag="VGB88_02545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9646349.1"
                     /translation="RVREVSVTVVSMTGFARRQGQDARHGWTWELKSVNHRAFDLRCR
                     LPQGLDALEPELRRRTAARIRRGSVTASLALAHGAGGAGVALNRPLLDQVLAIAARLG
                     EEITLRPAAIDGLLAIPGMIQPVEEDAEAVAARDAAILASFEDGLEALCGARAAEGAR
                     LEPVLARLLDDMAALADQARAAAAAQPAAIRARLEAQIGELLAVAPALPEERLAQEVA
                     LLAVKGDVREEIDRLCAHVGAAQGLLGDAEPVGRQLDFLCQELNREANTLCSKAADVA
                     LTRIGLALKATIDQLREQVQNIE"
     gene            899..1561
                     /gene="gmk"
                     /locus_tag="VGB88_02550"
     CDS             899..1561
                     /gene="gmk"
                     /locus_tag="VGB88_02550"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9646350.1"
                     /translation="MSDSRDQPPAAPAELKRRGLALVLSSPSGAGKTTISRRLLAQDE
                     NLRLSVSVTTRPKRPDEAAGVDYRFVDRRAFQAMVEAGELLEHARVFDHDYGTPRAPV
                     ETALAEGRDVLVDIDWQGAQQLSEAMGEDVVKVFILPPSTAELARRLTLRAQDPPEVV
                     QKRMAKAAGEMTHWAEYDYVVINRDLERAVAEVAAVLCAERLRRDRYLDLSRFVGGLT
                     AG"
     gene            complement(1577..2440)
                     /gene="rsmA"
                     /locus_tag="VGB88_02555"
     CDS             complement(1577..2440)
                     /gene="rsmA"
                     /locus_tag="VGB88_02555"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9646351.1"
                     /translation="MSQGRFDASALPPIREVIARHGLRAEKRLGQHFLHDGNLCARIV
                     RAAAPLDGAEVIEVGPGPGGLTRALLASPARAVIAIERDARCIAALDELAAAAGARLR
                     LIAADALAVDVLALAQPPRAVVANLPYNIATALLVRWLEAVARDAGALRSMTVMLQKE
                     VADRLAAAAGGRTYGRLSVLAQWLCEVRPAFTVPARAFTPPPKVTSTLLRLDPRPRPL
                     CPADAGALQRVCAAAFGQRRKMIRTSLKQLPVDAARLLDAAGIAGELRAEQVGVAGFC
                     ALARALEQAER"
     gene            complement(2457..3800)
                     /locus_tag="VGB88_02560"
     CDS             complement(2457..3800)
                     /locus_tag="VGB88_02560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9646352.1"
                     /translation="MSEYPGITVETAREPMVRVTGRERLAWFARRSALAAVLLGLASV
                     GAGAEDLMRIAAVVNDKVISVYDLDSRVAMALASSKLPDTAEVRQQVTGPVLRNLIDE
                     ALKVQEADRQGVRVLDSEFAAAMDEVARNNGVAREALPDFLSKVGVSIGALESQVRAQ
                     IAWGKFVSQRLRPTIDVSDEDIQAEIDRLEANRGQPEYLVSEIDLYLDPQTSEQDLIA
                     TANGLVGQIRNGTDFAAIARQFSQGAMAPSGGNVGWIREGQSRPEIDQALRTMEVGSV
                     SDPIRSLDGLHIISLRDKRAVMAEETADSDAEVYLSQILLPAAADEPAGAADDRLARA
                     NQVRAGLAGCKAMAARASELESSLSGDIGWLKMQDLPEEFRATVAGLEVDQPSAPIVT
                     SAGVHVLMVCDRREPGGARDIRDLIYDRLAAQRLAILERRLLRDLRRNAFVDLRN"
     gene            complement(3912..6065)
                     /gene="lptD"
                     /locus_tag="VGB88_02565"
     CDS             complement(3912..6065)
                     /gene="lptD"
                     /locus_tag="VGB88_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX9646353.1"
                     /translation="MTRASRIAAALAAVVVASLAHLAQPAPALAQTEDDVVLLADQVG
                     YDERLGLVTASGSVELARGGRVLRSDRLTYNKRSDLVTASGNVALLETDGTVVFADYV
                     ELTGDLKSGAIRGLGILLTDGSRLAASGGRMTADGRTALSKVVYSPCELCEDDPDRAP
                     LWQLKAYRVVHDKRLHTIFYQDAFLELFGIPVLYTPFFSHPDPTVERKTGFLPPTFGR
                     NSTLGITYTQPYFFNISPSRDATIAPTMTEREGPVLTGEYREATDSGYYELAGSITRG
                     SRLAEDTRPGDRTRGHGSAHGKFSINPYWRIGFDAERSTDATYLARYGFQRGLTTLVQ
                     DAYLQGRRGREHADIDLLYFQSLDPDVDEGTVPYVLPLFDYGWRSAPGIAGGVWLFDG
                     NARVMGRSEGTDSRRLSVKGGWQLPHIGRMGDIYELELSLRGDMYHVNGQQAEAGTRP
                     PEDGFTGRVVPQASLGWRWPWVGEYGSLRPIVEPIGQLVFSPNGGNSTDIPNEDSLSF
                     EFDDANLFSPTRFPGLDRVESGFRANYGVRFALYGEGDAWSELFLGQAYRQRDDDTFE
                     AGTGLSRRFSDYVGRLTVAPSDFVDLNLRFRLDRENLAARRASVSATAGPEWLRLSMS
                     YVTLSDTPTTGVPSSVEQISAAASYRFLDNWRLFASHVHDLAVGGGSLVTAGGLAYGD
                     ECIDVSVRASRHFTRELDIEPDTTFTFTVRLRNLG"
     gene            complement(6062..7168)
                     /gene="lptG"
                     /locus_tag="VGB88_02570"
     CDS             complement(6062..7168)
                     /gene="lptG"
                     /locus_tag="VGB88_02570"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX9646354.1"
                     /translation="MRVSATLSTYLARQFLVGMAIAFVALATLIFLVDLVELGRRASG
                     AATAGVWLVAEMASLHLPYLAQRVVPYAVLIGTMLTLTRLTRTNELAVARAAGVATWQ
                     ILLPGLGLAVALGVFMMAVFNPLASAMLSRYELLDARNLRGQESKLALSSSGLWLRQA
                     DDFGEAVIHAQRISQPGLVLHDVIIFRYQGTDRFVERIDAATARLEHGYWLLSEALIT
                     GPGRPSERRADYEFETQLTDSQIQESFGSPETLSFWRIPGFIALLERAGFSALKHRLH
                     WHSVLSLPVLLAGMVLVGATFSSRLPRRGASGLMVGVGVLVGFALYVFSDVVTALGLS
                     ASIPVLLAAWAPAAVALLAGAAMLLHARMLEGAP"
     gene            complement(7173..8297)
                     /gene="lptF"
                     /locus_tag="VGB88_02575"
     CDS             complement(7173..8297)
                     /gene="lptF"
                     /locus_tag="VGB88_02575"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX9646355.1"
                     /translation="MHRHTRYIITQLIGPFVVVTLSLTGVVWLTQSLRFVDLIVNKGL
                     SIGLFLTLTVLITPSLLGIIMPIALFASTLYVYHRLTIDSEIVVLRAMGLSNWQLAAP
                     ALALGIMVMAAVLAINLYLMPAGFRVFKDRQFEIRNSYASVLLQEGVFNTPVEGLTIY
                     VRERRSSGELSGIMVHDERDPVEPATVMAERGTLVATPEGPRFVLANGSRQQIDVANG
                     SLSMLYFDSYAFDFGPMPEFGPARFREAKERYFHELLYPGPGAEERHVREFMAEAHAR
                     LIGCLQPLALALIATAFLLPGEFNRRGEGWRILAAVGAAIAFLAVVHSLQGMVSREPW
                     LVPSYYLASFAAGAAAAFALISDGRRRRRPPAEPMPVRIG"
     gene            8492..9994
                     /locus_tag="VGB88_02580"
     CDS             8492..9994
                     /locus_tag="VGB88_02580"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9646356.1"
                     /translation="MNLSFSGTSLDGTGALALGVFEGRALAGRAGELDRATGGAIRRA
                     LDHARFEGKTGQSLTILAPARIKAGRVVLLGLGDPKDFGTLAAAKAGGAAVAQLAMSG
                     ETAAALVVETPKQAKVDEATAAAHAAHGARLRSYRFDKYRTTEKKDQKPSLARMTVMC
                     AQPAKARALYRPLDALGDGVFLTRNLVSEPANVLNPPSYAKEIRKLAGEGLKVEVLAE
                     PRLKRMGMGALLAVGAGSAHDSQVVVIRWNGAPQRARRQRPIAFIGKGVTFDTGGISI
                     KPAQGMEDMKWDMGGSAVVVGLMLALARRKAKVDAVGVVGLVENMPSGTAQRPGDVVT
                     SYSGQTIEVLNTDAEGRLVLADVLWYAQKRFKPRFMVDLATLTGAIIVSLGNVRAGLF
                     SNDDALSKRLVAAGDEVGELVWPMPLDEPYDKLLKSDIADVKNIGGRGAGSITGAKFL
                     QRFVNKLPWAHLDIAGVTWTQEDSELVPKGGSAFGVRLLDRLVASHYEAT"
     gene            10005..>10405
                     /locus_tag="VGB88_02585"
     CDS             10005..>10405
                     /locus_tag="VGB88_02585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004347.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX9646357.1"
                     /translation="MAGAGTTDIGFYQLNRSPLERALPRLVERALDGGFRVLIRCGSR
                     PRLDQLNGVLWTYDPGSFLPHGGPDDGNAADQPVYLSDGPDNPNGARLLVLVDGVEAA
                     DLDRFERCLDLFDGTDPEALEAARRRWRALK"
BASE COUNT         1608 a         3633 c         3760 g         1404 t
ORIGIN      
        1 tgcgggtccg ggaggtgtcc gtgaccgtcg tcagcatgac cgggttcgcc cgccgccagg
       61 gacaagacgc gcggcacggc tggacctggg agctgaaaag cgtcaaccac cgcgccttcg
      121 acctgcgctg ccgcctgccg cagggcctgg atgcgctgga gcccgagctg cgccggcgca
      181 cggcggcgcg gattcgccgc ggcagcgtca ccgccagcct cgcgctcgca catggcgcag
      241 gcggcgccgg ggtggcgctg aaccgcccac tgctggacca ggtgctggcg atcgccgcgc
      301 gcctcggcga ggagatcacg ctcaggccgg cggcgatcga cgggctgctc gccatccccg
      361 gcatgatcca gccggtcgag gaggacgccg aggcggtggc ggcgcgcgat gccgcgatcc
      421 tggcgagctt cgaggacggg ctggaggcgc tgtgcggcgc gcgcgcggcg gaaggggcaa
      481 ggctcgagcc ggtgctcgcg cgcctgctcg acgacatggc cgcgctcgcg gatcaggcgc
      541 gcgcggccgc cgccgcccag cccgcggcaa tccgcgcccg cctcgaggcc cagatcggtg
      601 agctgctggc ggtcgcgccg gcgctgcccg aggagcggct ggcgcaggag gtggcgctgc
      661 tcgcggtcaa gggcgacgtg cgcgaggaga tcgaccggct gtgcgcccat gtcggcgccg
      721 cccagggcct gctcggcgac gccgagccgg tcggccggca gctcgacttc ctgtgccagg
      781 agctcaaccg cgaggccaac accttgtgct cgaaggcggc cgacgtggcg ctcacccgca
      841 tcggcctggc gctgaaggcg accatcgacc agctgcgcga gcaggtccag aacatcgaat
      901 gagcgactct cgcgaccagc caccggccgc gccggccgag ctcaagcgcc gcgggctcgc
      961 gctggtgctg tcctcgccgt cgggggccgg caagaccacc atctcgcgcc gcctgctcgc
     1021 ccaggacgaa aatctgcggc tgtcggtgtc ggtgaccacg cgcccgaagc ggcccgacga
     1081 ggcggcgggc gtcgactacc ggttcgtcga ccggcgcgcg ttccaggcca tggtcgaggc
     1141 cggcgagctg cttgagcacg cgcgtgtgtt cgaccacgac tacggcaccc cgcgcgcgcc
     1201 ggtcgagaca gcgctcgccg aagggcgcga cgtgctggtc gacatcgact ggcagggcgc
     1261 gcagcagctc agcgaggcga tgggcgagga cgtggtcaag gtgttcatcc tgccgccctc
     1321 gaccgcggag ctcgcccgcc gtctcacgct ccgcgcccag gatccgcccg aggtggtgca
     1381 aaagcgcatg gccaaggcgg ccggcgagat gacccattgg gcggagtacg actacgtggt
     1441 gatcaaccgc gacctcgagc gagcggtcgc cgaggtcgcg gccgtgctgt gcgccgaacg
     1501 gctgcggcgc gaccgttacc tcgacctcag ccggttcgtg ggcgggctga cggcgggctg
     1561 agctggcgca gggcgctcac cgctcggcct gctcgagcgc gcgggcgagg gcacagaagc
     1621 cggcaacgcc gacctgctcg gcgcgcagct cgccggcgat gccggccgcg tccagcagcc
     1681 gggcggcgtc gaccggaagc tgcttgaggc tggtgcggat cattttccgg cgctggccga
     1741 acgccgccgc gcacacccgc tgcaatgcgc cggcatccgc cggacacagc ggcctggggc
     1801 gcggatcgag ccggagcaga gtcgaggtca ccttgggcgg cggcgtgaac gcgcgcgccg
     1861 gaaccgtgaa cgccgggcgc acctcgcaca gccactgggc gagaaccgac aggcgcccgt
     1921 aggtgcgccc gcccgcagcc gcggccagcc ggtcggcgac ctccttctgg agcatcaccg
     1981 tcatcgagcg gagcgcgccc gcgtccctcg ccaccgcctc gagccagcgg accagcagcg
     2041 cggtggcgat gttgtagggc aggttggcga ccaccgcgcg cggcggctgg gcgagcgcca
     2101 gcacatcgac cgcgagcgca tcggccgcga tcaggcgcag gcgcgcgccg gcggcggcgg
     2161 caagctcgtc caatgccgct atgcagcgcg catcgcgctc gatcgcgatc accgcccgcg
     2221 ccgggctcgc caggagcgcc cgcgtcaggc cgcccgggcc cggcccgacc tcgatcacct
     2281 cggcaccgtc gagcggcgcc gcggcgcgca cgatgcgcgc gcacaagttg ccgtcgtgca
     2341 ggaaatgctg tcccaaccgc ttctcggccc gcaagccgtg gcgggcgatg acctcgcgga
     2401 tcggcggcag cgcggaggcg tcgaaccggc cctggctcaa gggacggcgt cgccggtcaa
     2461 ttgcgcaggt cgacgaaggc gttgcggcgc aggtcgcgca acaggcgccg ctccaggatc
     2521 gccaggcgct gggccgccag gcggtcgtag atgaggtcgc ggatgtcgcg cgctccgccg
     2581 ggctcgcgcc ggtcgcagac catcagcaca tggaccccgg ccgaggtcac gatcggcgcg
     2641 ctcggctggt cgacctcgag cccggcgacg gtggcgcgga actcctcggg caggtcctgc
     2701 atcttgagcc agccgatgtc tcccgacagc gaggactcga gctcggaggc gcgcgccgcc
     2761 atcgccttgc agccggcaag cccggcgcgc acctggttcg cgcgggcgag cctgtcatcg
     2821 gccgccccgg ccggctcgtc ggccgccgcc ggcagcagga tctgcgacag gtagacctcg
     2881 gcatcgctgt ccgcggtctc ctctgccatc accgcgcgct tgtcgcgcaa cgagatgatg
     2941 tgcagcccgt cgagcgagcg gatcgggtcg gacacgctgc ccacctccat ggtgcgcagc
     3001 gcctggtcga tctccggccg gctctggcct tcgcggatcc agcccacatt gccgcccgag
     3061 ggcgccatcg ctccctggct gaactgacgc gcgatggcgg cgaagtcggt gccgttcctg
     3121 atctgcccga ccagcccgtt ggcggtcgcg atcaggtcct gctcgctggt ctgcggatcc
     3181 aggtagagat cgatctcgga caccaggtat tccggctgcc cgcggttggc ctcgaggcgg
     3241 tcgatctcgg cctggatgtc ctcgtcgctg acgtcgatgg tcggacgcag ccgctggcta
     3301 acaaacttgc cccaggcgat ctgggcgcga acctggcttt cgagcgcgcc gatgctgacg
     3361 ccgaccttgg acaggaagtc cggcaaggcc tcgcgcgcga ccccgttgtt gcgcgccacc
     3421 tcgtccattg ccgccgcgaa ctccgagtcg agcacgcgca cgccctggcg gtccgcttcc
     3481 tggaccttga gcgcctcgtc gatcagattg cgcagcaccg gcccggtgac ctgctggcgc
     3541 acctcggccg tatcgggaag cttcgagctc gccagcgcca tcgcgacccg ggaatcgagg
     3601 tcgtagaccg aaatcacctt gtcgttgacc accgcggcga tgcgcatcag gtcctcggca
     3661 cctgcgccga cggacgccag gccgagcagg acggcggcca acgcactgcg gcgggcgaac
     3721 cacgccaggc gctcgcgccc ggtcacgcga accatcggct cccgcgccgt ctcgaccgtg
     3781 atacctgggt actcggacat gggttcgatc cgctcaggcg gcggctcacg aagtgtcgac
     3841 tggcgccggt cggggccggc gccgtctggt atatggtgtc gacttcgacg ctgcaaccgg
     3901 tggcaggcgt ctcaacccag attgcgcagg cgcacggtga aggtgaacgt ggtatcgggc
     3961 tcgatatcga gctcgcgcgt gaagtggcgg ctcgcgcgca cgctcacgtc gatgcactcg
     4021 tcgccatatg ctaagccacc ggcggtgacc aggctgccgc cgccgaccgc gaggtcgtgg
     4081 acgtggctcg cgaacagtcg ccagttgtcg aggaacctgt aactcgcggc ggccgaaatc
     4141 tgctcgactg aactcggcac cccggtggtc ggcgtgtcgc tgagggtgac gtaggacatg
     4201 ctgagccgca gccattccgg cccggcggtt gcgctcaccg acgcccgacg cgccgccagg
     4261 ttctcgcggt ccaggcggaa cctcagattg agatcgacga aatcgctggg cgccaccgtc
     4321 agccggccga catagtcgga gaagcggcgc gacaagccgg tgccggcctc gaaggtgtcg
     4381 tcgtcgcgct ggcgataggc ctggccgagg aacagttccg accaggcatc gccttcgcca
     4441 taaagcgcga agcggacgcc gtagttggcg cgaaagccgc tctcgacgcg gtcgaggccg
     4501 ggaaagcgcg tcgggctgaa caggttggcg tcgtcgaact cgaatgacag gctgtcctcg
     4561 ttcgggatgt cggtggaatt gccgccgttc ggactgaaca ccaactgtcc aatcggctcg
     4621 acgatcgggc gcagggagcc gtactcgccg acccacggcc agcgccagcc gagcgaggcc
     4681 tggggcacca cgcggccggt gaagccgtcc tcgggtggcc gcgtgcccgc ctccgcctgc
     4741 tggccgttga cgtgatacat gtcgccgcgc agcgacagct cgagctcgta gatgtcgccc
     4801 atgcgcccga tgtggggcag ctgccagccg cccttcaccg acaatcttcg gctgtcggtg
     4861 ccttcgctgc ggcccatcac ccgggcgttg ccgtcgaaca gccagacgcc gccggcgatc
     4921 ccgggcgcgc tgcgccagcc atagtcgaac agcggcagca cgtagggcac cgtgccttcg
     4981 tcaacgtccg gatcgaggct ctggaagtag agcaggtcga tatcggcgtg ctcgcgcccg
     5041 cgacggccct gcaaataggc gtcctggacg agcgtggtga gcccgcgctg gaagccgtag
     5101 cgggcgaggt aggtggcatc ggtcgagcgc tcggcatcga agcctatgcg ccaatacggg
     5161 ttgatcgaga acttgccgtg ggcgctgccg tggccgcgcg tccgatcgcc cgggcgcgta
     5221 tcctcggcca gccgcgagcc gcgggtgatg ctgccggcca gctcgtaata gccgctgtcg
     5281 gtcgcctcgc ggtactcgcc ggtcaggacc gggccctcgc gctcggtcat ggtcggcgcg
     5341 atggtcgcgt cccggctggg cgagatgttg aagaaatagg gctgggtgta agtgatgccg
     5401 agcgtcgaat tgcggccgaa ggtcggcggc aggaagccgg tcttgcgctc caccgtcggg
     5461 tcggggtggc tgaagaacgg cgtgtacagg accggaatcc cgaacagctc gaggaaggca
     5521 tcctggtaga agatcgtgtg caggcgcttg tcgtggacca cgcggtaggc cttgagctgc
     5581 cacaacggcg cccggtccgg gtcgtcctcg cacagctcgc acgggctgta gaccaccttg
     5641 ctcaaggcgg tgcggccgtc cgccgtcatc cgcccgccgc tggcggccag gcgcgagccg
     5701 tcggtgagca ggatgccgag gccccggatc gcgccgctct tgaggtcgcc ggtcagctcg
     5761 acatagtcgg caaacaccac cgtgccgtcg gtctcgagca gggcgacgtt gcccgaggcg
     5821 gtgaccaggt cgctgcgctt gttgtaggtc aacctgtccg agcgcagcac gcgcccgccg
     5881 cgggcaagct cgaccgagcc gctcgccgtg accagcccga ggcgctcgtc gtagccgacc
     5941 tggtcggcca gcagcaccac gtcgtcctcg gtctgggcaa gcgcgggcgc gggctgggcg
     6001 aggtgggcga gagacgccac gaccacggcg gcgagcgcgg ccgcgatccg actcgcccgg
     6061 gtcatggcgc gccttcgagc atgcgcgcgt ggagcagcat cgccgcgccc gccagcagcg
     6121 ccaccgcggc cggcgcccag gcggcgagca gcaccgggat gctggccgaa aggccgagcg
     6181 ccgtgaccac gtcggagaac acgtacagcg cgaatccgac cagaaccccg accccgacca
     6241 tcaggccgct ggcgccgcgc cgcggcaggc gcgaggagaa ggtggcgccg accaggacca
     6301 tgccggcgag cagcaccggc agcgacagca cggagtgcca gtggaggcgg tgcttgagcg
     6361 ccgagaaccc ggcccgctcc agcagtgcga tgaagcccgg aatgcgccag aacgacagcg
     6421 tctcgggcga gccgaagctt tcctgaatct ggctgtcggt aagctgggtc tcgaactcat
     6481 agtccgcgcg ccgctcggac gggcgcccgg ggccggtgat cagtgcctcg ctcagcaacc
     6541 agtagccgtg ctcgaggcgg gcggtcgcgg cgtcgatccg ctcgacgaac cggtccgtgc
     6601 cctggtagcg gaagatgatc acgtcgtgca gcaccaggcc gggttgcgag atgcgctggg
     6661 cgtggatcac cgcttcgccg aaatcgtcgg cctgcctgag ccacaggccc gagctggaca
     6721 gcgcgagctt cgattcctgg ccgcgcagat tgcgggcgtc gagcagctcg tagcgcgaca
     6781 gcatcgccga ggcgagcgga ttgaacaccg ccatcatgaa caccccgagc gcgacggcga
     6841 ggccgagccc gggcaacagg atctgccagg ttgcgacgcc ggcggcgcgc gccaccgcaa
     6901 gctcgttggt ccgggtcagc cgggtgagcg tgagcatggt gccgatcagc acggcatacg
     6961 gcacgacgcg ctgggcaaga tagggcaagt gcaggctcgc catctcggcg accagccaga
     7021 ccccggcagt cgcggcgccg ctcgcccgcc ggcccagctc gaccaggtcg accaggaaga
     7081 tcagcgtcgc cagcgcgacg aaggcgatcg ccatcccgac caggaactgg cgggcgagat
     7141 aggtggacag ggtggcggag acgcgcaccg cctcagccga tccgcaccgg catcggctcg
     7201 gccggcgggc gccggcggcg gcgcccgtcg ctgatcagcg cgaaggcggc ggcggcgccg
     7261 gccgcgaagc tcgccaggta atagctcggc accagccagg gctcgcggct gaccatgcct
     7321 tgcaggctgt ggacgaccgc caggaatgcg atcgcggccc cgaccgcggc caggatgcgc
     7381 cagccctcgc cgcggcggtt gaactctccg ggcaacagga acgcggtcgc gatcaacgcc
     7441 agcgccagcg gctgcaggca gccgatgagg cgcgcatgcg cctcggccat gaactcgcgc
     7501 acatgccgct cctcggcgcc ggggccgggg tacagcagct cgtggaagta gcgctccttg
     7561 gcctcccgga accgggcggg cccgaactcg ggcatcggcc cgaaatcgaa ggcgtaggag
     7621 tcgaaataca gcatcgacag gctgccgttc gcgacgtcga tctgctggcg gctgccgttg
     7681 gcgagcacga agcgcggacc ctcgggcgtg gcgaccaacg tgccgcgctc ggccatcacc
     7741 gtcgccggct cgaccgggtc gcgctcgtca tggaccatga tgccgctgag ctcgccgctc
     7801 gaccggcgct cgcggacata gatcgtcagg ccctcgaccg gggtgttgaa cacgccctcc
     7861 tgaagcagca ccgaggcgta ggagttgcga atctcgaact gccggtcctt gaacacgcgg
     7921 aagcccgccg gcatcaggta aagattgatc gccagcaccg cggccatgac catgatcccg
     7981 agcgccagcg ccggcgccgc cagctgccag ttcgacagcc ccatcgcgcg cagcacgacg
     8041 atctcggaat cgatggtcag gcggtgatag acgtaaagcg tgctggcgaa cagcgcgatc
     8101 ggcatgatga tgccgagcag cgacggggtg atcagcacgg tcagggtcag gaacagcccg
     8161 atcgacaggc ccttgttgac gatcaggtct acgaagcgca gcgactgggt cagccagacg
     8221 accccggtga ggctcaaggt gacgaccacg aacgggccga tcagctgggt gatgatatag
     8281 cgcgtgtggc ggtgcatccg tcccccggca gccgaaccgg tgcggcgacg aaagccccga
     8341 gcggggcgcg gcgccgccta gccgcggaat taaactaaat ctttttttgt ccgcaacaat
     8401 cgctaaagtg cgcgcccggc cgcggggcct ccgactggtc gccgatcgtc gcccggcgcg
     8461 gctcccgcgc gatgtcatcg tagggagacc catgaacctg agcttttccg ggacctcgct
     8521 cgacggcaca ggcgcattgg cgctcggcgt gttcgagggg cgcgcgctgg cgggccgcgc
     8581 cggcgagctc gaccgggcca cgggcggggc gattcgccgc gccctcgacc atgcgcgatt
     8641 cgagggcaag accgggcagt cgttgacgat cctggcgccg gcgcggatca aggccggccg
     8701 cgtggtgctg ctgggcctgg gcgacccgaa ggacttcggc acgctcgccg ccgccaaggc
     8761 gggcggcgcc gcggtcgcgc aactggcgat gtcgggcgag accgcggcgg cgctggtggt
     8821 cgagaccccg aagcaggcga aggtcgacga ggccaccgcc gccgcccacg ccgcccatgg
     8881 cgcaaggttg cgcagctatc gattcgacaa gtaccggacc acggagaaga aggaccagaa
     8941 gccgtcgctc gcgcgcatga cggtgatgtg cgcgcagccg gccaaggcgc gcgcgctgta
     9001 ccggccgctg gatgccctcg gcgacggagt gttcctgacc cgcaacctgg tgtcggagcc
     9061 cgccaacgtc ctcaacccgc cgagctacgc caaggagatc cgcaagctgg cgggcgaggg
     9121 cctcaaggtc gaggtgctgg cggagccgcg cctgaagagg atgggcatgg gcgcgctgct
     9181 cgcggtcggc gccggcagcg cgcacgactc gcaggtggtg gtgatccgct ggaacggcgc
     9241 gccgcaacgc gcccggcgcc agcgcccgat cgcgttcatc ggcaagggcg tcaccttcga
     9301 caccggcggc atctcgatca agcccgccca gggcatggag gacatgaagt gggacatggg
     9361 cgggtccgcg gtggtggtcg ggctgatgct ggcgctcgcc cggcgcaagg ccaaggtcga
     9421 tgcggtcggc gtggtcggcc tggtcgagaa catgccgtcg ggaaccgcgc agcggccggg
     9481 cgacgtggtc acctcgtact ccggccagac gatcgaggtg ctcaacaccg atgccgaggg
     9541 ccggctggtg ctcgccgacg tgctgtggta cgcgcaaaag cggttcaagc cgcggttcat
     9601 ggtcgacctc gccacgctca ccggcgccat catcgtctcg ctcggcaacg tccgcgcggg
     9661 cctgttctcg aacgacgacg ccttgagcaa gcgcctggtc gccgccggcg acgaggtcgg
     9721 cgagctggtc tggccgatgc cgctcgacga gccctacgac aagctgctca agtccgacat
     9781 cgctgacgtc aagaacatcg gcggccgcgg cgcgggcagc atcaccggcg ccaagttcct
     9841 gcagcgtttc gtcaacaagc tgccgtgggc gcatctcgac atcgccggcg tgacctggac
     9901 gcaggaggac tcggagctgg tgcccaaagg cggctcggcg ttcggcgtgc gcctgctcga
     9961 ccggctggtg gcgagccact acgaggcgac gtagcgggct tcgcgtggcc ggcgcgggca
    10021 ccaccgacat cggcttctat cagctcaacc gctcgccgct tgagcgcgcc ttgccgcgcc
    10081 tggtcgagcg cgcgctggac ggcggcttcc gggtgctgat ccgctgcggc tcgcgcccgc
    10141 gcctcgacca gctcaacggc gtgctatgga cctacgaccc cggcagcttt ttgccgcatg
    10201 gcggccccga cgacggcaac gccgccgacc agccggtcta tctgagcgac gggcccgaca
    10261 accccaacgg cgccaggctg ctggtcctgg tcgacggcgt cgaggcggcc gacctcgacc
    10321 gcttcgagcg ctgcctcgac ctgttcgacg gcaccgaccc ggaggcgctg gaggcggcgc
    10381 gccggcgctg gcgcgcgctc aagga
//
LOCUS       DASWHU010000071         5434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_26205, whole genome shotgun sequence.
ACCESSION   DASWHU010000071 DASWHU010000000
VERSION     DASWHU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26205"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VGB88_02590"
     CDS             <1..706
                     /locus_tag="VGB88_02590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9646358.1"
                     /translation="HQTSFFTSHEALLLGYEQALTRVDSTTGDWYDCSAHMLWIGDRT
                     RDPDEAHVEFLRGVGNPLGIKVGPSCDADTLIRLIDILNPDNQAGRLTLIARMGTDKV
                     AKLLPPLVRRVAREGRTVAWACDPMHGNTIKSSSGYKTRPFDRILAEVEDFFAVHEAE
                     GSYAGGVHFEMTGRDVTECIGGAQAITEDGLSDRYHTHCDPRLNASQSLELAFLIAAE
                     LKSRRLVRRADVAAAS"
     gene            766..1962
                     /locus_tag="VGB88_02595"
     CDS             766..1962
                     /locus_tag="VGB88_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX9646359.1"
                     /translation="MASPGEPVAPGDSDFEAELGRRTDHYFKRTRDIVRRYGDIRATY
                     ALFMRRPVIFCPRLAVEWLERAASERATRFDIELCFEEGAWVGAGEPLMYVTGPLSEL
                     VDLETIYLQLLGPPCVAAYNAFTMCADMPGVAFLAMDARHCAGAEMAEMMAYAASVGS
                     TKARREANAIGFIGNSNDETAHYFGNRVGLGTMPHALLGFAGSTVRAAEMFHDTYPDE
                     PLTVLVDYFGCEVSDSIAVGERFPDLARDGRLRVRVDTHGGRFVEGLDTARSYDVLER
                     HAPQAVRTYRSETELRWLVGTGVTAASIFHLRDALDQAGFGRIGIVASSGFNPGKCKV
                     FASVAAPVDMIGTGSYLPERWSETYATADIVAYDGRPSVKVGREFLLRRPGQAEGEGE
                     GEDAES"
     gene            complement(1959..2636)
                     /locus_tag="VGB88_02600"
     CDS             complement(1959..2636)
                     /locus_tag="VGB88_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEX9646360.1"
                     /translation="MPKATDVVTAFATPILVRDIADATAVNAALKATILKRESASRGV
                     HQSNAGGWHSRPDLLSWTNPEVTQLRRWLLAGIADITTASGKVAANAITVRTLIAWAN
                     VSRRGNYNKLHEHPRSDWSGVYYVAAGSPPKPGQESGNIEFLDPRGAVGTRGTPGQGF
                     GVKLKFQPRDGMMLIFPSWVWHLVQPYDGAEERISIAFNADVERASARRRIVATASPG
                     QRPKRLH"
     gene            2762..4426
                     /locus_tag="VGB88_02605"
     CDS             2762..4426
                     /locus_tag="VGB88_02605"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX9646361.1"
                     /translation="MPDTLSLALAQVNPVVGDLAGNARMVREARAEAGRLGADLVVLP
                     ELVVTGYPPEDLVLRRAFQVAVAREVERLAAETADGGPAVLLGAPWRDGDALYNAALL
                     LDQGRIAARRYKHKLPNYGVFDEVRVFRAGPVPGPVPFRGLRLGVMVCEDMWTADVAE
                     TLEECGAQVLVVINGSPYERGKEDERLSLAVARVTETGLPLAYVNQVGGQDELVFDGA
                     SFVLDGDRSLRVQAPSWAECVRLSAWTAGAGERLTCADGVRDPQPEGSEQIYTALATG
                     LRDYVNKNGFPGVIIGLSGGIDSALTAAVAVDALGLERVRGVRMPSRYSSGHSLADAA
                     ACAAALGIPCETVPIEPAHAAFEQMLAPAFEGYQPDTTEENIQARVRGVILMALSNKT
                     GAMLVTTGNKSEMSVGYATLYGDMCGGYSVLKDVYKTTAYELARWRNRHRPAAGLGPE
                     AAVIPDHVITKAPSAELKPDQTDQDSLPPYPVLDDILESLVENEMSLDEIVARGHERA
                     LVKRVRRMLYVAEYKRRQAPPGVKITRRAFGRDRRYPITNGFIALE"
     gene            4602..>5434
                     /gene="cysS"
                     /locus_tag="VGB88_02610"
     CDS             4602..>5434
                     /gene="cysS"
                     /locus_tag="VGB88_02610"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9646362.1"
                     /translation="MQLRLTNTASGIKEPFTPLDPGHVRMYVCGPTVYDRAHIGNARP
                     VVVFDVLYRLLKRHYPRVTYVRNITDIDDKIIAAAKETGEPIPELTRRTTEMFHADMA
                     ALGALDPDAEPRATDNIPQMIAMIEALLASGHAYPADGHVLFHVPSQADYGALSRRHR
                     EEMIAGARIEPAPYKKDPADFVLWKPSSPDQPGWDSPWGRGRPGWHLECSAMSERYLG
                     ETFDIHGGGQDLIFPHHENERAQSIAAHGGKPFVRYWVHNAYVIVNGEKMSKSLGNFF
                     TV"
BASE COUNT          821 a         1929 c         1880 g          804 t
ORIGIN      
        1 ccaccagacc agcttcttca ccagccacga ggcgctgttg ctcggctacg agcaggcgct
       61 gacccgcgtc gacagcacca ccggcgactg gtacgactgc tcggcgcaca tgctgtggat
      121 cggcgaccgc acgcgcgacc cggacgaggc ccatgtcgag ttcctgcgcg gggtcggcaa
      181 cccgctcggg atcaaggtcg ggccgagctg cgacgcggac acgctgatcc gcctgatcga
      241 cattctcaat cccgacaacc aggccggacg gctgaccctg atcgcgcgaa tgggcaccga
      301 caaggtcgcc aagctgctgc cgccgctggt ccggcgcgtg gcgcgcgagg ggcgcacggt
      361 ggcgtgggca tgcgacccga tgcacggcaa caccatcaag tcctcctcgg gctacaagac
      421 ccgcccgttc gaccgcatcc tcgccgaggt cgaggacttc ttcgccgtcc acgaggccga
      481 gggcagctat gccggggggg tccatttcga gatgaccggg cgcgacgtga ccgagtgcat
      541 cggcggcgcc caggcgatca ccgaggacgg cctgtcggac cgctaccaca ctcattgcga
      601 cccgcgcctg aacgcctcgc aaagcctcga gctcgcgttc ctgatcgcgg ccgagctgaa
      661 aagccgccgg ctggtgcggc gggcggacgt ggctgccgcc tcgtgacccg cgcgcggggg
      721 cacggcgtgg acccgcacgt cggcgcctcc cgcaggcgtc ctgagatggc ctcgcccggc
      781 gagccggtgg cgcccggcga cagcgatttc gaggccgagc ttggccgccg taccgaccat
      841 tacttcaagc gcacccgcga cattgtccgc cgctacggcg acatccgcgc cacctacgcc
      901 ctgttcatgc gccggccggt gatcttctgc ccgcggctgg cggtggagtg gctcgagcgc
      961 gccgccagcg agcgcgcgac caggttcgac atcgagctct gcttcgagga aggcgcctgg
     1021 gtcggcgccg gcgagccgct gatgtacgtc accgggccgc tgtcggagtt ggtcgacctg
     1081 gagaccatct atctgcagct gctggggccg ccctgcgtcg ccgcctacaa cgcgttcacc
     1141 atgtgcgccg acatgcccgg ggtcgccttc ctggcgatgg acgcgcgcca ctgcgccggt
     1201 gccgagatgg ccgagatgat ggcctacgcc gccagcgtcg gctcgactaa ggcacggcgc
     1261 gaggccaacg ccatcggctt catcggcaat tccaacgacg agaccgcgca ctacttcggc
     1321 aaccgcgtcg ggctcggcac catgccgcac gcgctgctcg gcttcgccgg ctcgaccgtg
     1381 cgcgcggccg agatgttcca cgacacctac ccggacgagc cgctgaccgt gctggtcgac
     1441 tatttcggct gcgaggtaag cgactcgatc gcggtcggcg agcgctttcc cgacctcgcc
     1501 cgcgacggcc ggctcagggt gcgcgtggat acccatggcg ggcgcttcgt cgagggcctc
     1561 gataccgccc gctcctacga cgtgctcgag cgccacgcgc cccaggcggt gcgcacctat
     1621 cgcagtgaga ccgagctgcg ctggctggtc ggcaccgggg tcaccgcggc gtcgatcttc
     1681 catttgcgcg acgccctcga ccaggccggc ttcggcagga tcgggatcgt cgccagctcc
     1741 ggcttcaacc cgggcaagtg caaggtgttc gcgtccgtcg cggcgccggt cgacatgatc
     1801 ggcaccggct cgtacctgcc cgagcggtgg tcggagactt acgccaccgc cgacatcgtc
     1861 gcctatgacg gccggccctc ggtcaaggtc gggcgcgagt tcctgctcag gcggccgggg
     1921 caggccgagg gcgagggcga gggcgaggac gccgagtcct aatgcaggcg tttcggcctc
     1981 tggccgggcg aggccgtggc gacgattcgg cgccgggccg aggcccgctc cacgtccgcg
     2041 ttgaaggcga tcgagatgcg ctcctcggcg ccgtcatagg gctggacgag gtgccacacc
     2101 catgacggga agatcagcat catgccgtcg cgcggctgga acttgagctt gacgccgaag
     2161 ccttgccccg gcgtgccccg cgtgccgacc gcgccgcgcg ggtccaggaa ctcgatgttg
     2221 ccgctctcct ggccgggctt gggcgggctg cccgcggcca cgtagtagac ccccgaccag
     2281 tccgagcgcg ggtgctcgtg cagcttgttg tagttgccgc ggcggctgac attggcccag
     2341 gcgatcagcg tgcgcaccgt gatggcgttg gccgcgacct tgccggaggc ggtggtgatg
     2401 tcggcgatgc cggccagcag ccaacgcctg agctgggtca cctccgggtt ggtccacgac
     2461 aacaggtcgg gccgcgaatg ccagccgccg gcattgctct ggtgcacgcc ccgcgaggcg
     2521 ctctcgcgct tgaggatggt cgccttgagc gccgcgttca ccgccgtggc gtcggcgatg
     2581 tcgcgcacca ggatcggggt ggcgaaggcg gtgacgacgt cggtggcctt gggcacggac
     2641 ttgggtcctg ctgtttggtc aagcgattgc gcagggctaa gaaaatgcgc gccttggccg
     2701 cgttgacaag ggcaggcggg cggcactaaa gctccgccaa ccgcgccgga ggctgcctgt
     2761 catgcccgat accctgtcgc tcgctctcgc ccaggtcaat ccggtggtcg gcgacctcgc
     2821 cggcaatgcc cggatggtcc gcgaggcgcg cgccgaggcc ggccggctcg gcgccgacct
     2881 ggtggtactg cccgagctgg tcgtcaccgg ctatccgccc gaggacctgg tgctcaggcg
     2941 cgcgttccag gtggcggtgg cgcgcgaggt cgaaaggctc gccgccgaga ccgccgacgg
     3001 cggcccggcg gtgctgctgg gcgcgccgtg gcgggacggc gatgcgctct acaacgccgc
     3061 cctgctgctc gaccagggcc gcatcgccgc gcgccgctac aagcacaagc tgcccaacta
     3121 cggcgtgttc gacgaggtgc gggtgttccg cgccggcccg gtgcccgggc cggtgccgtt
     3181 ccgcggcctc aggctcggcg tgatggtgtg cgaggacatg tggaccgccg acgtcgccga
     3241 gacgctcgag gaatgcggcg ctcaggtcct ggtggtgatc aacggctcgc cctacgagcg
     3301 cggcaaggag gacgagcggc tgtcgctcgc ggtcgcgcgg gtcaccgaga ccggcctgcc
     3361 gctcgcctac gtcaaccagg tcggcggcca ggacgagctg gtgttcgacg gcgcctcgtt
     3421 cgtgctcgac ggcgaccgca gcttgcgggt gcaggcgccg tcatgggccg aatgcgtgcg
     3481 cctgtccgcg tggaccgccg gcgccggcga gcgcttgacc tgcgccgacg gcgtgcgcga
     3541 cccccagccc gagggcagcg agcagatcta caccgcgctc gccaccggcc tgcgcgacta
     3601 cgtcaacaag aacggcttcc cgggcgtgat catcggcctg tcgggcggca tcgactccgc
     3661 cctcaccgcc gcggtcgcgg tcgatgcgct ggggctggag cgggtgcgcg gcgtacgcat
     3721 gccgtcccgc tactcctccg gccacagcct cgccgacgcc gcggcctgcg cggcggcgct
     3781 cggcatcccc tgcgagacgg tgccgatcga gcccgcgcac gccgctttcg agcagatgct
     3841 cgcgcccgcg ttcgagggct atcagccgga caccaccgag gagaacatcc aggcgcgcgt
     3901 gcgcggcgtg atcctgatgg cgctgtcgaa caagaccggc gcgatgctgg tcaccaccgg
     3961 caacaagtcc gagatgtcgg tcggctacgc caccctctac ggcgacatgt gcggcggcta
     4021 ctcggtgctc aaggacgtct acaagaccac cgcctatgag ctcgcccgct ggcgcaaccg
     4081 gcaccggccc gcggccggcc tcgggcccga ggcggcggtg atcccggatc acgtcatcac
     4141 caaggcgccg tcggccgagc tcaagcccga ccagaccgac caggattcgt tgccgcccta
     4201 tccggtgctc gacgacatcc tcgaatccct ggtcgagaac gagatgtcgc tggacgagat
     4261 cgtcgcccgc ggccacgagc gcgcactggt caagcgcgtg cgccggatgc tctatgtcgc
     4321 cgagtacaag cgccgccagg cgccgcccgg ggtcaagatc acccggcgcg cgttcggccg
     4381 cgaccgccgc tacccgatca ccaacggctt catcgccctc gaatagcccc gcgcatgttg
     4441 ccgtcattcc ggccggaccc cggcgtcaag ccggggggag agccggaatc catgcttcga
     4501 tggaccccgc tttcgcgggg gtgacgactg gggtaggagc ttgtggtcaa gttggaaacg
     4561 actctgcaat cgaggaatca ggcgctaaaa gagacaacac catgcagctt cgtctcacca
     4621 acaccgcgag cgggatcaag gagccgttca cgccgctcga tcccgggcac gtgcggatgt
     4681 acgtgtgcgg gccgacggtc tatgaccgcg cgcatatcgg caacgcccgc ccggtggtgg
     4741 tatttgacgt gctctaccgc ttgctcaagc gccattaccc gcgcgtcacc tatgtgcgca
     4801 acatcaccga catcgacgac aagatcatcg ccgcggcgaa ggagaccggt gagccgatcc
     4861 cggagctgac ccggcgcacg accgagatgt tccacgccga catggccgcc ctcggcgcgc
     4921 tcgatcccga cgcggagccg cgcgcgaccg acaacatccc gcagatgatc gcgatgatcg
     4981 aggctctgct ggcgtccggt cacgcctacc cggctgacgg ccacgtgctg ttccatgtgc
     5041 catcgcaggc ggactacggc gcgctgtcgc ggcgccaccg cgaggagatg atcgccggcg
     5101 cgcgcatcga gccggcgccc tacaagaagg acccggccga cttcgtgctg tggaagccct
     5161 cgagcccgga ccagccgggc tgggactcgc cctgggggcg ggggcggccg ggctggcacc
     5221 tggaatgctc ggcaatgagc gagcgctatc tcggcgagac cttcgacatc cacggcggcg
     5281 gccaggacct gatctttccc catcacgaga acgagcgcgc ccagagcatc gccgcccacg
     5341 gcggcaagcc gttcgtacgc tactgggtgc acaacgccta cgtcatcgtc aacggcgaga
     5401 agatgtcgaa gagcctcggc aacttcttca ccgt
//
LOCUS       DASWHU010000072         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_768966, whole genome shotgun sequence.
ACCESSION   DASWHU010000072 DASWHU010000000
VERSION     DASWHU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768966"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VGB88_02615"
     CDS             <1..1233
                     /locus_tag="VGB88_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9646363.1"
                     /translation="LALLGGLILNLMPCVLPVLSLKLLGVIGHGGGERRRVTLSFVAS
                     AAGIVVSFLVLAGAAVAVKLAGGAVGWGMQFQQPGFLVFVILVLGLFAYNLIGLYQVR
                     LPGWLGDLGLAAGDRAERLGGGLAGHFMTGAFATLLATPCSAPFLGTAVSFALGRGAG
                     EILAVFAALGLGMSAPYLVVAAFPGLATRLPRPGRWMVWVLWALAIALLGTAAWLVTV
                     LAAVAGWPAAGTVAGVLLLAAPAIRIARAQGALRPLGAGALAVLLAAAFAAPAALESP
                     APQSRPGATAEAVDWRPFDADAIAPAVAAGQVVFVDVTADWCVTCKVNKRVVLDSDEV
                     AARLTAPWVVRMRADWTRPDQRIADYLASFDRYGIPFNAVYGPGVPDGRALSELLTRV
                     EVSHALDAARGPVAAAID"
     gene            1382..1864
                     /locus_tag="VGB88_02620"
     CDS             1382..1864
                     /locus_tag="VGB88_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9646364.1"
                     /translation="MAIKVGDRIPTVNLRYMAADGIKEMSTKELFGGKKVVVFALPGA
                     FTPTCSAKHLPGFVQHADEIKAKGVDAIACIAVNDAFVMGAWGKNQNAGDKVIMLADG
                     NADFTKAVGLELDATGFGMGLRSQRYAMVVDNGVVKTLAVEDGPGLDVSAAEKILAKL
                     "
BASE COUNT          212 a          659 c          707 g          287 t
ORIGIN      
        1 ctggcgctgc tcggcgggct gatcctcaac ctgatgccgt gcgtgctgcc ggtgctgtcg
       61 ctcaagctgc tcggcgtcat cggccacggc ggcggcgagc ggcggcgggt gacgctgagc
      121 ttcgtcgcct cggcggccgg gatcgtggtc tcgttcctgg tcctggccgg ggccgcggtc
      181 gcggtcaagc tcgccggcgg cgcggtcggc tggggcatgc agttccagca gccggggttc
      241 ctggtgttcg tgatcctggt gctgggcctg ttcgcctaca acctgatcgg gctctaccag
      301 gtccgcctgc ccggctggct cggcgacctc gggcttgccg ccggcgatcg cgccgagcgc
      361 ctcggcggcg ggctcgccgg ccacttcatg accggcgcct tcgccaccct gctcgccacc
      421 ccgtgctcgg cgccgttcct cggcaccgcg gtgagcttcg cgctcggccg cggtgcgggc
      481 gagatcctcg ccgtcttcgc cgccctcggc ctcggcatgt cggcgccgta cctggtggtg
      541 gcggcgtttc ccggcctcgc cacccggctg ccgcggccgg ggcggtggat ggtctgggtg
      601 ctctgggcgc tcgcaatcgc gctgctcggc acggcggcgt ggctggtcac cgtgctcgcc
      661 gccgttgccg gctggccggc tgcgggcacg gtcgccgggg tgctgctgct cgccgcgccg
      721 gcgatccgca tcgcgcgcgc ccaaggcgcg ctgcgcccgc tcggcgccgg ggcgttggcg
      781 gtgctgctgg cggcggcgtt cgcggcaccg gcggcgctcg aatccccggc accgcaaagc
      841 cggcccggcg ccaccgccga ggcggtcgac tggcgcccgt tcgatgcgga cgcgatcgcg
      901 ccggcggtgg ccgcgggcca ggtggtgttc gtcgacgtca ccgccgactg gtgcgtgacc
      961 tgcaaggtaa acaagcgcgt ggtgctcgac tccgacgagg tcgcggcgcg gctcacggcg
     1021 ccgtgggtgg tgcgcatgcg cgccgactgg acccgccccg accagcgcat cgccgactat
     1081 ctggcgagct tcgaccgcta cggcatcccg ttcaacgcgg tctacggccc cggcgtgccc
     1141 gacggccggg cgctgtccga gctgctcacc agggtcgagg tgtcgcacgc gctcgacgcc
     1201 gcgcgcgggc cggtcgcggc ggcgatcgac tgaccgcgta gcggccgtcg cctgccgggc
     1261 gggcgctgcg atttcgcttg gcctcggccg cgcaacgggt atttatctgg cggacgcgag
     1321 cgctcggggg cgcccggcgg gcgggtctcg gcgaccggaa ggcgagtgag gaggagcggc
     1381 aatggcgatc aaggtgggag acaggatccc gaccgtcaac ctgcgctaca tggcggcgga
     1441 cgggatcaag gagatgtcga ccaaggagct gttcggcggc aagaaggtgg tcgtgttcgc
     1501 gctccccggc gcgttcaccc cgacctgctc ggcaaagcac ctgcccggct tcgtccagca
     1561 tgccgacgag atcaaggcca agggcgtcga cgccatcgcc tgcatcgcgg tcaacgacgc
     1621 cttcgtgatg ggcgcctggg gcaagaacca gaacgccggc gacaaggtga tcatgctcgc
     1681 cgacggcaac gccgacttca ccaaggcggt cgggctcgag ctcgacgcga ccggattcgg
     1741 catgggcctg cgctcgcagc gctatgcgat ggtggtcgac aacggcgtgg tcaagacgct
     1801 cgcggtcgag gacggccccg gcctcgacgt ctcggcggcg gagaagatcc tggccaagct
     1861 gtagc
//
LOCUS       DASWHU010000073        16798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_26459, whole genome shotgun sequence.
ACCESSION   DASWHU010000073 DASWHU010000000
VERSION     DASWHU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26459"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VGB88_02625"
     CDS             complement(<1..118)
                     /locus_tag="VGB88_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9646365.1"
                     /translation="MIGRLNHVAIAVPDLEAARALYRDTLGAQVSAPVAMPEH"
     gene            complement(222..1868)
                     /locus_tag="VGB88_02630"
     CDS             complement(222..1868)
                     /locus_tag="VGB88_02630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9646366.1"
                     /translation="MTAQGELLFVPLGGAGEIGMNLNLYGLDGKWLMLDCGVSFADEW
                     LPGVDLVMPDPAFIEARRDALLAIVLTHAHEDHLGAVPHLWPRLGCPVYATPFAAAVL
                     RRKLAEVGLEEAVDLRIVPLKSRFEIGPFDLELITVTHSILEPNAVALRTRLGTILHT
                     GDFKIDPDPLIGEETDAAALAALGEEGVLAMVCDSTNVFRPGESGSEGELRRTLDELI
                     RGREGRVVVTSFASNAARIETVAKCALRNGRHVALAGRSLWRIVEAAREAGYLRDLPP
                     LLPDNEAGFLPRDKVLVLATGCQGEPRAAMAKIAADEHPHIALDPGDLVVFSSKIIPG
                     NEKAIFRMHNRLVGRGIEVVTEADHFVHVSGHPAREELARMYRWVRPRIAVPVHGERR
                     HIAEHAAFARSLQVPQAIEVENGCVVRLAPGEAGIVDHVESGRLALDGNTLLPVDSEV
                     MRGRRRLMVAGVVFVTLVMDRAGKLQAAPTVRAEGVFADGAEAGEELSELVRDAVEAL
                     PRARRGDDETVRETARLSMRREIQRRHDKRPMVAVELVRL"
     gene            complement(1865..2641)
                     /locus_tag="VGB88_02635"
     CDS             complement(1865..2641)
                     /locus_tag="VGB88_02635"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9646367.1"
                     /translation="MSAPKLLAIDAGNTNIVFAVFDGDRLLHQWRAATVTNRTADEYA
                     VWLTQLMGLKGLASAEVGAAIIACVVPQALHELKQLCRRYFACEAQVVGEGLDIGIEV
                     RVERPGDVGADRLVNAIAAHHRHAGWLIVIDFGTATTFDVVADDGGYEGGVIAPGVNL
                     SLEALDRAAAKLPRIAVQKPQRVIGSGTVTAMQSGVYWGYVGLVEGLVRRIKGEIDRP
                     AMTIATGGLAPLFAGATEAIDVVEPDLTLHGLSRLHLLNR"
     gene            complement(2638..3399)
                     /locus_tag="VGB88_02640"
     CDS             complement(2638..3399)
                     /locus_tag="VGB88_02640"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9646368.1"
                     /translation="MTRAPKLPPPFSLVALESVDSTNSEARRRAEAGAPDGTVVWARS
                     QSAGRGRRGRAWQSPPGNLYVSVLLRPDLDAADCARLTFLVAVALGDALAGLLPPLAT
                     IAHKWPNDVLVNGRKCAGILIESATSGGALVDWLVIGVGVNLAHHPGDAEFPATDLAF
                     EGAGEVTPEAALEAFARHLLRWLDVWGQDGFAPVRAAWLARAWRIGEPIEVRLDSRRL
                     SGRFERLDDDGALIVAHGDGSRTRIEAGAVFPPTP"
     gene            complement(3396..4835)
                     /gene="nuoN"
                     /locus_tag="VGB88_02645"
     CDS             complement(3396..4835)
                     /gene="nuoN"
                     /locus_tag="VGB88_02645"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX9646369.1"
                     /translation="MTAFPDLSPALAEVVLLATALVLLMAGAFRKGEGEGPASLIALA
                     GMIIAFLAVAFSPAGETTTFAGLFVADAFARFIKALILLGAVLTMAIGQGYRARARML
                     QFEYPVLIVFATLGMLMMVSANDLIALYVAIELHSLPLYVIAAFRRDMRKSTEAGLKY
                     FILGALSSGMLLYGCSLVYGFTGATGFGAIAEAVAGGAPSLGLLVGLVFIAAGLAFKA
                     SAVPFHMWTPDVYEGAPTVVTAFLTAAPKVAAVALILRVLVEPFGGLVGEWRQIVVFI
                     SIASMALGAFAAIWQTNIKRLMAYSSIGNVGYALVGLAAGTEQGVQAVIIFMAIYLVM
                     TIGTFACILAMRVQGEMVESIANLAGLGRTRPMMALALGIFMFSLAGIPPLAGFFGKL
                     YVFLAAVQAGLYTLAVIGVLTSVVGAFYYVRIVKLMYFDDPAEAFDRPMERELSLVIG
                     GTSVAILLFFFYLAPIVAGAAGAAAALFP"
     gene            complement(4832..6343)
                     /locus_tag="VGB88_02650"
     CDS             complement(4832..6343)
                     /locus_tag="VGB88_02650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9646370.1"
                     /translation="MTSWPLLSLIVFLPLVGAMLVFLIRGEAEVVARNARAVALWTSL
                     MTLALALMLWAAFDPATAAFQFVERAEWMPGLGVSYYLGVDGISLLFVVLSSFLVPIC
                     VLASWRAITERVKEYMVAFLVMGSMMNGVFCSLDIVLFYVFFEGILIPMFLIIGVWGG
                     PRRVYAAFKFFLYTLAGSVLSLVAILAMYFTAGTAEIPMLMSAGFDPALQYWLWLALF
                     ASFAVKVPMWPLHTWLPDAHVEAPTAGSVILAGVLLKLGGYGFIRFSLPMLPEASAFF
                     TPFVYGLSVIAVVYTSLVALMQTDMKKLIAYSSVAHMGFVTIGLFTLTTQGVEGAMMV
                     MLSHGVVSAALFLCVGVVYDRRHSRDIASYGGLVHRMPAYALVFMVFTLASVGLPGTS
                     GFVGELLVLIGAFQANTWVAALAATGVILGAAYMLWLYRRVFFGQLTKDELKAILDLD
                     RREVAVFAPLVAVVLWMGIYPDSFLNVMHASVDNLLAQSQPIAATAPLDLAGQ"
     gene            complement(6346..8271)
                     /gene="nuoL"
                     /locus_tag="VGB88_02655"
     CDS             complement(6346..8271)
                     /gene="nuoL"
                     /locus_tag="VGB88_02655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9646371.1"
                     /translation="MIVLTVLLPLAAALIAGLLGRVIGARGAQAVTCGALVLSAVLGT
                     VIFARAIGGEAPYTVELFTWMSSGGLDFSWALYVDQLTAIMVFVVTVVSALVHVYSVG
                     YMAHDPHVPRFMAYLSLFTFAMLALVTADNFVQLYFGWEGVGLCSYLLIGYWYTRPAA
                     NAAAIKAFVVNRVGDFGFGLGIFAIFMVFDSLDYATVFASAPALDGTTVGFLGVEVDS
                     LTLICLLLFMGAMGKSAQIGLHTWLPDAMEGPTPVSALIHAATMVTAGIFMVARTSPL
                     FELSETALAVVTVVGAATAFFAATIALVQNDIKRVIAYSTCSQLGYMFLACGVSAYAA
                     GIFHLMTHAFFKALLFLAAGSVIHAMSDEQDMRKMGGIWRMIPVTYGLMWVGSLALAG
                     IPPFAGFFSKDAILEAAFAAGSVVGDLAYWLGAGAAAMTAFYSWRLLFMTFHGAARAD
                     EKVMAHVHESPPVMIVPLAGLALGAVVAGFLGLGLVDPEGGYWGASLSVAAGHGALAA
                     AHHAPVWVKGLPLALALGGIAVAWQLYIRRPEIPARLAASARGVYLFLLNKWYFDELY
                     DRLFVAPAKRIGLGLWKSGDGEVIDGLGPDGIAAAVVDLARRASRLQTGYVYHYAFVM
                     LVGVALLVTWYMIGAGG"
     gene            complement(8278..8586)
                     /gene="nuoK"
                     /locus_tag="VGB88_02660"
     CDS             complement(8278..8586)
                     /gene="nuoK"
                     /locus_tag="VGB88_02660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9646372.1"
                     /translation="MAVTLTHYLVVAAILFTLGVFGIFLNRKNVIVILMSIELMLLAV
                     NVNLVAFSTHLGDMVGQVFAMFVLTVAAAEVAIGLAILVVYNRVRGTIEVDDINLMKG
                     "
     gene            complement(8586..9200)
                     /locus_tag="VGB88_02665"
     CDS             complement(8586..9200)
                     /locus_tag="VGB88_02665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9646373.1"
                     /translation="MILPTICFYLFAGVMVAAGVMVISARNPVHSVLFLILAFLNAAG
                     LFVLMGAEFLAMILAIVYVGAVAVLFMFVVMMLDIDLAQMREGFLQYLPIGGMIGLVL
                     LVELGFALAAWRASAGDGAEPLVPTPPAGEVTNTEALGRLLYTDYFYLFQAAGVILLI
                     AMVGAIVLALRHREGVRRQSIARQVARRREDAVELRDVTPGSGI"
     gene            complement(9233..9721)
                     /gene="nuoI"
                     /locus_tag="VGB88_02670"
     CDS             complement(9233..9721)
                     /gene="nuoI"
                     /locus_tag="VGB88_02670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX9646374.1"
                     /translation="MSFVERGTRALLLTELVSGLWLTLGYLFRRKVTLNYPYEKGPLS
                     PRFRGEHALRRYANGEERCIACKLCEAICPALAITIEAEPREDGSRRTTRYDIDMTKC
                     IYCGYCQEACPVDAIVQGPNYEFATETREELFYDKDKLLANGDRWEKQIAANLEADAP
                     YR"
     gene            complement(9750..10760)
                     /gene="nuoH"
                     /locus_tag="VGB88_02675"
     CDS             complement(9750..10760)
                     /gene="nuoH"
                     /locus_tag="VGB88_02675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9646375.1"
                     /translation="MDELWTSYLWPGAVLVGKVLLIVVPLLLAVAYLTYAERKVIGAI
                     QLRRGPNVVGPFGLLQPIADGVKLLVKETILPARANVGVFVLAPMITFTLSFVAWAVI
                     PFDDGWVLADINVGVLYLFAISSLGVYGVLMAGWASNSRYAFLGALRSAAQMVSYEVS
                     IGFVIVTVLICAGSLRLSDIVTAQRDLWFFIPLFPMFVIFFISALAETNRLPFDLPED
                     ESALVAGYFVEYSSMLFALFFLGEYAAMILMSGMTVVLFLGGWLPPFDIAPFTWIPGV
                     IWFGLKLSLVLFAFLWVRATFPRYRYDQLMRLGWKVFLPMSLLWVAVTAGAVVAFDLY
                     PG"
     gene            complement(10771..12834)
                     /gene="nuoG"
                     /locus_tag="VGB88_02680"
     CDS             complement(10771..12834)
                     /gene="nuoG"
                     /locus_tag="VGB88_02680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX9646376.1"
                     /translation="MPTLTIDGREITVEPGTTVLQACELAGKEIPRFCYHERLSIAGN
                     CRMCLVEMERSPKPIASCAMPAGDGMVIHTDTPGVKRAREGVMEFLLINHPLDCPICD
                     QGGECDLQDQAMAYGRDRSRFHEHKRAVEDKDFGPLIKTFMTRCIHCTRCVRFMSEVA
                     GVPELGALNRGEDMEIASVIARGLGSELSGNIIDLCPVGALTSKPYAFSARSWELRKT
                     ESIDVLDAVGSNIRVDSRGRQVMRILPRLNEAVNEEWISDKTRFAYDGLRHQRLDRPY
                     LREADGRLRPASWQEAFAGIARRLDGLTGDRFGAIAGDLADCESMCALKDLTAALGSP
                     NIDCRQDGARLRAAPRVSYLFNTTIAGIDAADACLLVGTNPRLEAPIINQRIRRRWLA
                     GGLRIGVIGERADLTYGTDHLGAGPATLAEVAGGAHDFCAALDKAERPMIVLGMGALA
                     HPDGGVVLGLARALAERFGLVGEDWNGFNVLHTAAARVGGLDLGLVPGDGGLDVAGMV
                     QAAGRGDLDVLYLLGADEIDTGALGRAFVVYQGHHGDRGAQRADVVLPGAAYTEKSAT
                     YVNTEGRAQRTRLAVFPPGDAREDWAILRALSGALGRPLPYDSIAALRARMAEQSPHL
                     NGLDQVARATRAPFGSEGAYDQAPFASPIANFYMTDPIGRASETMAQCSAAFAPRLAR
                     TGTGG"
     gene            complement(12845..14125)
                     /gene="nuoF"
                     /locus_tag="VGB88_02685"
     CDS             complement(12845..14125)
                     /gene="nuoF"
                     /locus_tag="VGB88_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9646377.1"
                     /translation="MLADSDRIFTNLYGTHGAGLKDARARGDWDDTKALLARGREAIV
                     DEVKASGLRGRGGAGFPTGLKWSFMPKQSDGRPRFLVVNADESEPGTCKDRDIIRHEP
                     HKLVEGTLLAAFAMGANTAYIYIRGEFFNEARILQRAIDEAAEAGLVGRDACGSGFDC
                     DIHLHRGAGAYICGEETALLESLEGKKGMPRLKPPFPASAGLYGCPTTVNNVETIAVA
                     PTILRRGAGWFTGIGRENNTGTKVFCISGHVETPCNVEEAMGVPLRELIERHAGGVRG
                     GWDKLLAVIPGGSSVPCLPRAICDHQIMDFDALRDAKSGLGTAAVIVMDTATDIVAAI
                     ARLSHFYKHESCGQCTPCREGTGWMWRVMERLVEGRAEIAEIDLLLEVSRQVEGHTIC
                     ALGDAAAWPIQGLIRHFRPELERRILARKTRAAA"
     gene            complement(14125..14742)
                     /gene="nuoE"
                     /locus_tag="VGB88_02690"
     CDS             complement(14125..14742)
                     /gene="nuoE"
                     /locus_tag="VGB88_02690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9646378.1"
                     /translation="MSARAFAADQLQPESFAFTPENERQAQAAIAKFPPGRQQSAVIP
                     LLDLAQRQHGGWLPRAAIRTVAEMLHMPEMRGLEVATFYTMFNLAPVGRHLVQLCRTT
                     PCWLRDSEGVKRACLAHLGVGLGGTTADGAFTVVEVECLGACVNAPVVQINDDFYEDL
                     DPAAMVRVLDDLTAGREVAPGSQDARRQLAAAVTGPAALVAERDG"
     gene            complement(14739..15917)
                     /locus_tag="VGB88_02695"
     CDS             complement(14739..15917)
                     /locus_tag="VGB88_02695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011256326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9646379.1"
                     /translation="MATTDIRTFTFNFGPQHPAAHGVLRLVLEMDGEVVDRADPHIGL
                     LHRGTEKLIEYKTYLQALPYFDRLDYVSIMAQEHCFSLAVEKLLGIEVPERAKYIRVL
                     FDEITRILNHCLQVSANGLDIGAMTPFLWLFEQREMLMEFYERVSGARMHAAYVRPGG
                     VHQDMPAGLPEDIAAWADGFPKVLDDVEELLTGNRIWKQRNVDIGVVTAEQALDWGFS
                     GPMLRGSGVPWDLRKAQPYAVYERMDFDIPVGKNGDCYDRYLVRLEEMRQSLRIIAQC
                     LDQMPDGPVVVEDNKIAPPKREQMKRSMEALIHHFKLYTEGYHVPAGETYTAVEAPKG
                     EFGVYLIADGTNRPYRCKIRAPGFAHLQGIDFMAKGHQLADLVACIATMDIVFGEVDR
                     "
     gene            complement(15917..16534)
                     /locus_tag="VGB88_02700"
     CDS             complement(15917..16534)
                     /locus_tag="VGB88_02700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9646380.1"
                     /translation="MNQALIDLGDYIVSAVPELAASPTVALDELCVRSTPAGVVRLLT
                     FLRDDPECRFRQLTDICGVDWPERVPRFDVVYHLLSHTQNQRVRVKVPVDDEAPVPSA
                     CAVFSSAGWYEREVWDLYGIFFSDHPDLRRILTDYGFDGHPLRKDFPLTGYVEVRYDE
                     EQKRVVYEPVRLTQEFRTFDFISPWEGARYVLPGDEKAEEGPKGG"
     gene            complement(16569..>16798)
                     /gene="nuoB"
                     /locus_tag="VGB88_02705"
     CDS             complement(16569..>16798)
                     /gene="nuoB"
                     /locus_tag="VGB88_02705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002719665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX9646381.1"
                     /translation="RKVYDQMAEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDI
                     YVPGCPPTAEALLYGILQLQKKIRRTGTLAR"
BASE COUNT         2837 a         5840 c         5859 g         2262 t
ORIGIN      
        1 cgtgctcggg catcgccacc ggcgccgaca cctgggcgcc gagcgtatcg cgatagagcg
       61 cgcgcgccgc ctcgagatcg ggcaccgcga tcgccacgtg attgagcctg ccgatcatgt
      121 gatccctgcc gcctggtatc cagcgacgcg ccgggcgccg cacgcccgat atagcatggc
      181 cgttcgattg ccggggcgcg agacgacctg agcgcgggcg cctacagccg caccagctcg
      241 accgcgacca tggggcgctt gtcgtggcgg cgctggatct cccggcgcat ggataaacgc
      301 gcggtctcgc gcacggtctc gtcgtcgccg cggcgcgcgc gcggcagcgc ctcgaccgcg
      361 tcgcggacca gctcggaaag ctcctcgccg gcctcggcac cgtccgcgaa cacgccctcg
      421 gcgcgcaccg tgggcgccgc ttgcagcttg cccgcccgat ccatgaccag ggtcacgaac
      481 accacgccgg ccaccatcag ccggcggcgg ccgcgcatca cctcgctgtc gaccggcagc
      541 agcgtgttgc cgtcgagcgc gaggcggccg ctctcgacat ggtcgacgat ccccgcctcg
      601 cccggcgcca ggcgcaccac gcagccgttc tcgacctcga tcgcctgcgg cacctgcagc
      661 gagcgcgcga aggcggcgtg ctcggcgatg tggcggcgtt cgccgtgcac cggcacagcg
      721 atgcgcgggc gcacccagcg gtacatgcgc gcgagttcct cgcgcgccgg atggcccgag
      781 acgtggacga agtggtcggc ctcggtgacc acctcgatgc cgcgcccgac cagccggttg
      841 tgcatgcgga agatcgcctt ctcgttgccg ggaatgatct tcgacgagaa caccaccagg
      901 tcgcccggat cgagcgcgat gtgcggatgc tcgtcggcgg cgatcttggc catcgccgcg
      961 cgcggctcgc cctggcagcc ggtggccagc accagcacct tgtcgcgcgg caggaacccg
     1021 gcctcgttgt cgggcagcag gggcggcagg tcgcggagat agccggcctc gcgcgccgcc
     1081 tcgacgatcc gccacaggct gcgcccggcg agcgcgacgt ggcggccgtt gcgcagcgcg
     1141 cacttggcca cggtctcgat gcgcgccgcg ttggaggcga agctggtcac caccacccgc
     1201 ccctcgcggc cccggatcag ctcatcgagc gtgcgccgca gctcgccctc cgagcccgac
     1261 tcgccggggc ggaagacgtt ggtcgaatcg cagaccatgg ccagcacccc ctcctcgccg
     1321 agcgcggcga gcgccgccgc gtcggtctcc tcgccgatca gcgggtcggg gtcgatcttg
     1381 aagtcgccgg tatgcaggat ggtgccgagc cgggtgcgca gcgcgaccgc gttgggttcg
     1441 aggatcgagt gcgtcaccgt gatcagctcg aggtcgaacg ggccgatctc gaaccggctc
     1501 ttgagcggca cgatgcgcag gtccaccgct tcctcgaggc cgacctcggc gagctttcgg
     1561 cgcagcacgg cggcggcgaa cggagtcgcg tacaccgggc agccgaggcg cggccacaga
     1621 tgcggcaccg cgcccagatg gtcctcatgg gcgtgggtga gcacgatcgc gagcagggcg
     1681 tcgcggcgcg cctcgatgaa cgcgggatct ggcatcacca ggtcgacgcc gggcaaccat
     1741 tcgtcggcga acgagacgcc gcagtcgagc atcagccact tgccgtcgag gccgtacagg
     1801 ttgaggttca tgccgatctc gccggccccg ccgagcggca cgaacaacag ctcgccctgg
     1861 gcggtcatcg gttcaggaga tgcagccggc tcaacccgtg cagcgtcagg tcgggctcga
     1921 ccacgtcgat ggcctcggtg gcgccggcga acagcggcgc caggccgccg gtggcgatgg
     1981 tcatcgccgg gcggtcgatc tcgcccttga tgcgccggac cagcccctcg accaggccga
     2041 catagcccca gtagacgccc gactgcatgg cggtgacggt gcccgagccg atcacccgct
     2101 gcggcttctg caccgcgatc cgcggcagct tggcggcggc gcggtcgagc gcctcgagcg
     2161 acaggttcac ccccggcgcg atcacgccgc cctcgtagcc gccgtcgtcg gcgaccacgt
     2221 cgaaggtggt ggcggtgccg aagtcgatca cgatcagcca gccggcatgc cgatggtggg
     2281 cggcgatcgc gttgaccagc cggtcggcgc cgacgtcgcc cgggcgctcg acgcgcacct
     2341 cgatgccgat gtcgagcccc tcgccgacca cctgggcctc gcacgcgaag tagcgccggc
     2401 acagctgctt gagctcgtgc agcgcctggg gcacgacgca ggcgatgatc gccgcgccga
     2461 cctcggccga cgccaggccc ttgaggccca tcagctgggt gagccagacc gcgtactcgt
     2521 cggcggtgcg gttggtcacg gtcgccgcgc gccattgatg cagcaggcgg tcgccgtcga
     2581 acaccgcgaa cacgatgttg gtgttgccgg cgtcgatcgc gagcagcttg ggcgcgctca
     2641 tggcgttggc gggaacaccg cgccggcctc gatccgcgtc cggctgccgt cgccgtgggc
     2701 gacgatcagc gcgccgtcgt cgtcgagccg ctcgaaccgg ccgctgagcc ggcgcgagtc
     2761 gagacgcacc tcgatcggct cgccgatgcg ccaggcgcgg gcgagccagg cggcgcgcac
     2821 cggcgcgaac ccgtcctgcc cccagacgtc gagccagcgc aacaagtggc gcgcgaacgc
     2881 ctcgagtgcc gcctccggcg tcacctcgcc cgcgccctcg aacgcgagat cggtcgccgg
     2941 gaactcggca tcgcccggat ggtgcgccag gttgacgccg acgccgatca ccagccagtc
     3001 gaccaacgcg ccgccgctgg tcgcggactc gatcaggatg ccggcgcact tgcggccgtt
     3061 gaccagcacg tcgttgggcc atttgtgggc gatggtggcg agcggcggca ggagccccgc
     3121 gagcgcatcc ccgagcgcga ccgcgaccag gaaggtgagc cgggcacagt cggcggcatc
     3181 gaggtcaggg cgcagcagga ccgaaacgta caggttgccg ggcggcgact gccaggcgcg
     3241 accgcggcgc ccgcgccccg cgctctggct tcgcgcccag accacggtgc cgtcgggcgc
     3301 gccggcctcg gcccgccggc gcgcttccga gttggtacta tcgaccgact cgagcgcgac
     3361 cagcgaaaac ggcggcggca gcttgggcgc gcgggtcacg gaaacagcgc cgcggcggcg
     3421 cccgccgcgc ccgcaacaat cggcgcgagg tagaagaaga acagcaggat cgccacgctg
     3481 gtgccgccga tcaccagcga cagctcgcgc tccatcggcc ggtcgaacgc ctcggccggg
     3541 tcgtcgaagt acatcagctt gacaatgcgc acatagtaga acgcgccgac cacgctggtc
     3601 agcacgccga tcacggccag cgtgtacagc cccgcctgga ccgccgcaag gaacacgtac
     3661 agcttgccga agaagccggc cagcggcggg atgccggcca gcgagaacat gaagatgccg
     3721 agcgcgagcg ccatcatcgg ccgggtgcgt cccagccccg caaggttggc gatggattcg
     3781 accatctcgc cctggacgcg catcgccagg atgcaggcga aggtgccgat ggtcatgacc
     3841 aggtagatcg ccatgaagat gatcaccgcc tggacgccct gctcggtgcc ggcggcgagc
     3901 ccgaccaggg catagccgac attgccgatc gagctgtacg ccatcagccg cttgatgttg
     3961 gtctgccaga tggcggcgaa ggcgccgagc gccatcgagg cgatcgagat gaataccacg
     4021 atctggcgcc actcgccgac caggccgccg aacggctcga ccaggacgcg caggatcagc
     4081 gccaccgccg ccaccttcgg cgccgcggtc aggaacgcgg tgaccacggt cggcgcacct
     4141 tcgtagacgt cgggcgtcca catgtggaac ggcaccgccg aggccttgaa cgcaagcccg
     4201 gcggcgatga acaccagccc gaccaggaga cccagcgaag gggccccgcc cgcgacggcc
     4261 tcggcgatcg cgccgaagcc ggtcgcgccg gtgaagccgt agaccagcga gcagccgtag
     4321 agcagcatgc cggacgacag cgcgccgagg atgaagtatt tgagcccggc ctcggtcgac
     4381 ttgcgcatgt cgcggcggaa cgcggcgatc acgtagagcg gcaggctgtg cagctcgatc
     4441 gcgacataga gcgcgatcag gtcgttggcc gagaccatca tcagcatgcc gagggtggcg
     4501 aacacgatca gcaccgggta ctcgaactgg agcatgcgcg cgcgcgcgcg gtagccctgg
     4561 ccgatcgcca tcgtcagcac cgcgccgagc aggatcagcg ccttgatgaa gcgggcgaag
     4621 gcgtcggcca cgaacaggcc ggcgaaggtg gtggtctcgc cggcgggcga gaacgcgacc
     4681 gcgaggaagg cgatgatcat gccggcgagc gcgatcaggc tcgccgggcc ctcgccctcg
     4741 cccttgcgaa aggcaccggc catcagcagc accagcgcgg tcgccagcag gacgacctcg
     4801 gccaaagccg gcgacaggtc gggaaacgcg gtcattgccc ggcgaggtcg agcggcgccg
     4861 tggccgcgat cggctgcgac tgcgccagca ggttgtcgac cgaggcgtgc atcacgttga
     4921 gaaagctgtc cggatagatg cccatccaca gcaccaccgc gaccagcggc gcgaacaccg
     4981 ccacctcgcg ccggtcgaga tcgaggatcg ccttgagctc gtccttggtg agctggccga
     5041 agaacacgcg ccggtacagc catagcatgt aggcggcgcc gaggatcacg ccggtcgcgg
     5101 cgagcgcggc gacccaggtg ttggcctgga acgcgccgat cagcaccagc agctcgccga
     5161 cgaagccgct ggtcccgggc aggccgacgg atgcgagcgt gaacaccatg aacaccagcg
     5221 cataggccgg catgcggtgg accagcccgc catagctcgc gatgtcgcgc gagtgccggc
     5281 ggtcgtagac cacgcccacg cacaggaaca gcgcggccga gaccacgccg tggctcagca
     5341 tcaccatcat cgcgccttcg acgccctggg tggtgagcgt gaacaggccg atggtgacaa
     5401 agcccatatg ggcgaccgac gagtaggcga tcagcttctt catgtcggtc tgcatcagcg
     5461 cgaccagcga ggtgtagacc accgcgatca cgctgaggcc gtacacgaac ggcgtgaaga
     5521 acgcgctcgc ctcgggcagc atcggcagcg agaaacggat gaagccgtag ccgccgagct
     5581 tgagcagcac gccggccagg atcaccgagc ccgcggtcgg cgcctcgaca tgggcgtccg
     5641 gcagccaggt gtgcagcggc cacatcggca ccttgaccgc gaacgaggcg aacaaggcga
     5701 gccacagcca gtattgcagg gccggatcga agcccgcgct catcaacatc gggatctcgg
     5761 cggtgccggc ggtgaagtac atggccagga tcgcgaccag cgacagcacc gatccggcga
     5821 gcgtgtagag gaagaacttg aacgcggcgt acacccgccg cggcccgccc cacaccccga
     5881 tgatcaggaa catcgggatc aggatgccct cgaagaagac gtagaacagc acgatgtcga
     5941 gcgagcagaa caccccgttc atcatgctgc ccatgaccag gaacgcgacc atgtactcct
     6001 tgacgcgctc ggtgatcgcc cgccagctgg ccagcacgca gatcggcacc aggaacgacg
     6061 acagcaccac gaacagcaac gagatgccgt cgacgccgag gtagtacgag accccgagcc
     6121 cgggcatcca ctcggcccgc tcgacgaact ggaaggcggc ggtcgccggg tcgaacgcgg
     6181 cccacagcat caacgccagc gccaacgtca tcagcgaggt ccacagcgcc accgcgcgcg
     6241 cgttgcgcgc caccacctcg gcctcgccgc ggatcaggaa caccagcatc gcgccgacca
     6301 gcggcaggaa cacgatcagg ctgagcagcg gccagctggt cacggtcagc ccccggcgcc
     6361 gatcatgtac caggtgacga gcagcgcgac tccgaccagc atgacgaaag catagtggta
     6421 gacgtaaccg gtctgcagcc ggctcgcgcg ccgggcaagg tcgaccaccg cggcggcgat
     6481 gccgtcggga ccgaggccgt cgatcacctc gccgtcgccc gacttccaca ggccgaggcc
     6541 gatgcgcttc gccggcgcga cgaacaggcg gtcgtagagc tcgtcgaagt accacttgtt
     6601 gagcaggaac aggtagacgc cgcgcgccga cgccgcgagc cgcgcaggga tctcgggccg
     6661 gcggatgtag agctgccagg cgacggcgat gccgccgagc gcaagcgcca gcggcagccc
     6721 cttgacccag accggcgcgt ggtgggcggc ggcgagcgcg ccgtggccgg cggcaacgct
     6781 taaggacgcg ccccagtagc cgccctcggg atcgaccagg ccgaggccga ggaatcccgc
     6841 gaccaccgcg ccgagcgcga ggcctgccag cggcacgatc atcaccggcg gcgattcatg
     6901 gacatgcgcc atcaccttct cgtcggcgcg cgcagcgccg tggaaggtca tgaacagcag
     6961 ccgccaggaa tagaacgcgg tcatcgccgc cgcgcccgcc cccagccagt aggcgaggtc
     7021 gccgaccacg ctgccggcgg cgaacgcggc ctccaggatc gcatccttgg agaagaagcc
     7081 ggcgaacggc gggatgccgg caagcgccag cgagcctacc cacatcagcc cgtaggtcac
     7141 cgggatcatg cgccagatgc cgcccatctt gcgcatgtcc tgctcgtccg acatggcgtg
     7201 gatcaccgag ccggcagcga ggaacaagag cgccttgaag aacgcatggg tcatcaggtg
     7261 gaagatgccg gcggcatatg cggagacgcc gcaggcgagg aacatgtagc cgagctggct
     7321 gcaggtcgag taggcgatca cgcgcttgat gtcgttctgc accagggcga tggtggcggc
     7381 gaagaatgcg gtcgcggcgc cgaccacggt gaccacggcg agcgcggtct cggacagctc
     7441 gaacaagggc gacgtgcgcg cgaccatgaa gatgcccgcg gtgaccatgg tcgcggcgtg
     7501 gatcagggcc gagaccggcg tcgggccctc catggcatcg ggcagccagg tgtgcagccc
     7561 aatctgcgcc gacttgccca tcgcgcccat gaacaagagc aggcagatca aggtcagcga
     7621 gtcgacctcg acgccgagga agccgaccgt ggtgccgtcg agcgcgggcg cgctcgcgaa
     7681 cacggtcgcg tagtcgagcg agtcgaagac catgaagatg gcgaagatgc cgaggccgaa
     7741 gccgaagtcg ccgacccggt tgacgacgaa cgccttgatc gcggcggcgt tcgccgccgg
     7801 gcgcgtgtac cagtagccga tcagcaggta gctgcacagc ccgacccctt cccagccgaa
     7861 atagagctgg acgaagttgt cggcggtgac cagcgccagc atggcgaagg tgaacagcga
     7921 caggtaggcc atgaaccgcg gcacgtgcgg atcatgcgcc atgtagccga ccgagtagac
     7981 gtggaccagc gccgagacca cggtgaccac gaacaccatg atcgcggtca gctggtcgac
     8041 gtagagcgcc cacgagaagt cgagcccgcc cgagctcatc caggtgaaca gctcgaccgt
     8101 gtacggcgcc tcgccgccga tcgcgcgcgc gaagatgacg gtgccgagga cggcggagag
     8161 caccagcgcg ccgcaggtca ccgcttgcgc gccgcgggcg ccgatcacgc gcccgagcag
     8221 gcccgcgatc agcgccgcgg ccagcggcag caggacggtg agaacgatca cctggcctca
     8281 acccttcatc aggttgatgt cgtcgacctc gatggtgccg cgcacccggt tgtagacgac
     8341 caggatggca agcccgatcg cgacctcggc ggcggcaacg gtgagtacga acatggcgaa
     8401 cacctgcccg accatgtcgc cgagatgggt cgagaacgcg accaggttga cgttgaccgc
     8461 aagcagcatc agctcgatcg acatcaggat cacgatcacg ttcttgcggt tgaggaagat
     8521 gccgaacacg ccgagcgtga acaggatcgc ggcgaccacc aggtaatggg tgagcgtcac
     8581 cgccatcaga tgccgctccc cggggtcacg tcgcgcagct ccaccgcgtc ctcgcgccgg
     8641 cgcgcgacct ggcgggcgat gctttgccgg cgcacgccct cgcggtggcg caacgccagc
     8701 acgatcgcgc cgaccatggc gatcagcagg atcacgcccg cggcctggaa caggtagaag
     8761 tagtcggtgt acagcagccg cccgagcgcc tcggtgttgg tcacctcgcc cgccggcggc
     8821 gtcggcacca gcggctcggc gccgtcgccg gcggacgccc gccaggcggc gagcgcgaag
     8881 ccgagctcga ccagcagcac cagcccgatc atgccgccga tcggcaggta ctggagaaag
     8941 ccctcgcgca tctgggcgag gtcgatgtcg agcatcatga ccacgaacat gaacagcacc
     9001 gccaccgcgc cgacatagac gatcgccagg atcatcgcca ggaactcggc gcccatcagc
     9061 acgaacagcc cggccgcgtt gaggaaggcg aggatcagga acagcaccga atgcaccggg
     9121 ttgcgggccg agatcaccat cacgcccgcc gccaccatga cgcccgcgaa caggtaaaag
     9181 cagatcgtcg gcaatatcat gatcggtccc gttccaccgc ccgtcgcgca gcctagcggt
     9241 acggcgcgtc ggcctccaga ttggcggcga tctgcttctc ccagcggtcg ccgttggcga
     9301 gcagcttgtc cttgtcgtag aacagctcct cgcgggtctc ggtggcgaac tcgtagttcg
     9361 gtccctggac gatggcgtcg accgggcacg cttcctggca gtagccgcag tagatgcact
     9421 tggtcatgtc gatgtcgtag cgggtggtcc gccggctgcc gtcttcgcgc ggctcggcct
     9481 cgatggtgat cgccagcgcc gggcagatcg cctcgcacag cttgcaggcg atgcaccgct
     9541 cctcgccgtt ggcatagcgc ctgagcgcgt gctcgccgcg gaagcgaggg ctcagcggcc
     9601 ccttctcgta cgggtagttg agcgtcacct tgcgccggaa caggtagccg agcgtgagcc
     9661 acagccccga gaccagctcg gtcagcagca gtgcgcgcgt gccacgttcg acgaacgaca
     9721 tcctcggcct ctcccgcgtc cccgcccggt cagcccggat acaggtcgaa cgccaccacc
     9781 gcgccggcgg tgaccgcgac ccacaacagc gacatcggca ggaacacctt ccagcccaac
     9841 cgcatcagct ggtcgtagcg gtagcgcggg aacgtcgccc gcacccacag gaaggcgaac
     9901 aggaccagcg acagcttgag cccgaaccag atcacgcccg gaatccaggt gaacggcgcg
     9961 atgtcgaacg gcggcagcca gccgcccagg aacagcacca cggtcatgcc gctcatcagg
    10021 atcatggccg cgtactcgcc gaggaagaac agcgcgaaca gcatcgagga gtactcgacg
    10081 aagtagcccg ccaccagcgc cgactcgtcc tcgggcaggt cgaacggcag gcggttggtc
    10141 tcggccagcg ccgagatgaa gaagatcacg aacatcggga acagcgggat gaagaaccac
    10201 aggtcccgct gcgcggtcac gatgtcgctg agcctgagcg agccggcgca gatcagaacg
    10261 gtgacgatga cgaagccgat cgagacctcg taggacacca tctgcgcggc cgagcgcagc
    10321 gcgccgagga aggcgtagcg cgagttgctc gcccagcccg ccatcagcac gccgtagacg
    10381 ccgagcgagg agatcgcgaa caggtacagc acgccgacat tgatgtcggc gagcacccag
    10441 ccgtcgtcga acgggatcac cgcccaggcg acgaagctca aggtgaaggt gatcatcggc
    10501 gccagcacga acaccccgac attggcgcgc gccggcagga tggtctcctt gaccagcagc
    10561 ttgaccccgt cggcgatcgg ctgcagcagc ccgaacggcc cgaccacgtt gggcccgcgg
    10621 cggagctgga tcgccccgat caccttgcgc tcggcatagg tcagataggc gacagcgagc
    10681 agcagcggca ccacgatcag cagcaccttg ccgaccagga ccgcgccggg ccacagatag
    10741 ctggtccaca gctcgtccat cgccggcccc tcagccgccg gtgccggtgc gcgcgaggcg
    10801 cggcgcgaac gcggcgctgc attgggccat ggtttcggac gcccggccga tcgggtcggt
    10861 catgtaaaag ttcgcgatcg gcgaggcgaa cggcgcctgg tcatatgcgc cctcgctgcc
    10921 gaacggcgcc cgggtggcgc gcgccacctg gtcgagcccg ttgaggtgcg gcgactgctc
    10981 ggccatgcgc gcgcgcagcg ccgcgatcga gtcgtagggc agcggcctgc cgagcgcgcc
    11041 cgacagcgcg cgcaggatgg cccagtcctc gcgcgcatcc ccgggcggga agaccgcgag
    11101 tcgcgtgcgc tgggcgcggc cctcggtatt gacgtaggtc gcgctcttct cggtgtaggc
    11161 ggcgccgggc agcacgacgt cggcgcgctg ggcgccgcgg tcgccgtgat ggccctgata
    11221 gacgacgaag gctcggccga gcgcgccggt gtcgatctcg tcggcgccga gcagatagag
    11281 cacgtcgaga tcgccccggc ccgccgcctg gaccatgccg gcgacgtcca agccgccatc
    11341 gcccggcacc aggccgaggt cgaggccgcc gacccgcgcg gcggccgtgt gcagcacgtt
    11401 gaagccgttc cagtcctcgc cgaccagccc gaaacgctcg gcgagcgcgc gcgcgagacc
    11461 cagcaccacg ccgccgtcgg ggtgcgcgag cgcgcccatg ccgagcacga tcatcggccg
    11521 ttcggccttg tccagggcgg cgcagaaatc gtgcgccccg cccgcgacct cggccaaggt
    11581 cgccggcccg gcgccgagat ggtcggtgcc gtaggtgagg tccgcgcgct cgccgatcac
    11641 gccaatgcgc aagccgccgg cgagccagcg ccggcggatg cgctggttga tgatcggcgc
    11701 ctcgaggcgc gggttggtgc cgaccagcag gcacgcatcg gcggcgtcga tgccggcgat
    11761 ggtggtgttg aacaggtagc tcacgcgcgg cgccgcccgg aggcgggcgc cgtcctggcg
    11821 gcagtcgatg ttgggcgagc cgagcgccgc ggtcaggtcc ttgagcgcgc acatcgactc
    11881 gcagtcggcg aggtcgccgg cgatggcgcc gaagcggtcg ccggtaaggc cgtcgaggcg
    11941 ccgggcgatg ccggcaaacg cctcctgcca gctcgccggg cgcaagcggc cgtccgcctc
    12001 gcgcaggtac ggccggtcga ggcgctggtg cctgagcccg tcataggcga agcgggtctt
    12061 gtcggagatc cactcctcgt tcaccgcctc gttcagccgc ggcaggatgc gcatcacctg
    12121 gcggccgcgc gaatcgacgc ggatgttgct gcccaccgcg tcgagcacgt cgatggattc
    12181 ggtcttgcgc agctcccagg agcgggcgct gaacgcatag ggcttcgagg tcagcgcgcc
    12241 gaccgggcac aggtcgatga tgttgcccga cagctccgag ccgagcccgc gcgcgatcac
    12301 gctcgcgatc tccatgtcct cgccgcggtt gagcgcgccg agctcgggca ccccggcgac
    12361 ctcgctcatg aaccggacgc aacgcgtgca atggatgcag cgggtcatga aggtcttgat
    12421 cagcgggccg aagtccttgt cctcgaccgc gcgcttgtgc tcgtggaagc ggctgcggtc
    12481 gcggccatag gccatcgcct ggtcctgcag gtcgcactcg ccgccctggt cgcagatcgg
    12541 gcagtcgagc ggatggttga tcagcaggaa ctccatcacc ccttcgcgcg cgcgcttgac
    12601 ccccggcgtg tcggtgtgaa tgaccatgcc gtcgccggcc ggcatggcgc aggacgcgat
    12661 cggcttcggc gagcgctcca tctcgaccag gcacatgcgg cagttgccgg cgatcgacag
    12721 gcgctcgtga tagcagaagc gcgggatctc cttgccggcg agctcgcagg cctggagcac
    12781 ggtcgtgccc ggctcgaccg tgatctcgcg gccgtcgatg gtcagggtcg gcatggcgct
    12841 tcgatcatgc cgcggcgcgc gtcttgcgcg cgaggatgcg gcgctcgagc tcgggccgga
    12901 agtgccggat caggccttga atgggccacg ccgcggcatc gccgagcgcg cagatggtat
    12961 ggccctcgac ctggcggctg acctccagca acaggtcgat ctcggcgatc tcggcgcgcc
    13021 cctcgaccag gcgctccatc acccgccaca tccagccggt gccctcgcgg cacggggtgc
    13081 actggccaca gctctcgtgc ttgtagaagt gcgacaggcg cgcgatcgcg gcgacgatgt
    13141 cggtggcggt gtccatcacg atcaccgctg cggtgccgag ccccgacttg gcgtcgcgca
    13201 gcgcgtcgaa gtccatgatc tgatggtcgc agatcgccct cggcaggcag ggaacggacg
    13261 agccgccggg aatcaccgcc aacagcttgt cccagccgcc gcgcacgccg cccgcgtggc
    13321 gctcgatcag ctcgcgcagc ggcacgccca tcgcctcctc gacgttgcac ggagtctcga
    13381 cgtggcccga gatgcagaac accttggtgc cggtgttgtt ctcgcgcccg atgccggtga
    13441 accagccggc gccgcggcgc aggatggtcg gcgccacggc gatggtctcg acattgttga
    13501 ccgtggtcgg gcagccgtac agcccggcgc tggcggggaa cggcggcttc agccgcggca
    13561 tgcccttctt gccctcgagg ctctcgagca gcgcggtctc ctcgccgcag atataggcgc
    13621 cggcgccgcg atggagatgg atgtcgcagt cgaagcccga gccgcacgcg tcgcggccca
    13681 cgagccccgc ctcggcggcc tcgtcgatcg cgcgttgcag gatgcgggcc tcgttgaaga
    13741 actcgccgcg gatatagatg taggcggtgt tcgcgcccat cgcgaaggcg gcgagcaggg
    13801 tgccctcgac cagcttgtgc ggctcgtggc ggatgatgtc gcggtccttg caggtgccgg
    13861 gctcgctttc gtcggcgttg accaccagga agcgcggccg gccgtcggac tgcttgggca
    13921 tgaacgacca tttgagcccg gtcgggaagc cggcgccgcc gcgcccgcgc aggcccgagg
    13981 ccttgacctc gtcgacgatc gcctcgcggc cgcgggcgag caatgccttg gtgtcgtccc
    14041 agtcgccgcg cgcccgcgcg tccttcagcc cggcaccgtg cgtgccgtaa aggttggtga
    14101 agatgcggtc gctgtcggcc agcatcagcc gtctctctcg gcgaccaggg cggccgggcc
    14161 ggtgaccgcg gccgcgagct gccggcgcgc gtcctgcgag cccggcgcca cctcgcgccc
    14221 cgccgtgagg tcgtcgagca cgcggaccat ggccgccgga tcgaggtcct cgtagaagtc
    14281 gtcgttgatc tggaccaccg gcgcgttgac gcacgcgccc aggcactcga cctcgaccac
    14341 ggtgaaggcg ccgtcggcgg tggtcccgcc gagcccgacg ccgagatggg cgaggcaggc
    14401 gcgcttgacg ccctcgctgt ccctcagcca gcacggcgtc gtgcggcaga gctgcaccag
    14461 atgccggccg accggggcga ggttgaacat ggtgtagaag gtcgccacct cgaggccgcg
    14521 catctcgggc atgtgcagca tctcggccac ggtgcggatt gccgccctcg gcagccagcc
    14581 gccgtgctgg cgctgggcga ggtcgagcag cgggatcacc gcgctctgct ggcggccggg
    14641 cgggaacttg gcgatcgcgg cctgcgcctg gcgctcgttc tcgggcgtga acgcgaacga
    14701 ctcgggctgc agctggtccg cggcgaaggc gcgcgcgctc accggtcgac ctccccgaac
    14761 acgatgtcca tggtcgcgat gcaggcgacc aggtcggcga gctgatggcc cttggccatg
    14821 aaatcgatgc cctgcaaatg agcgaatccg ggcgcgcgga tcttgcaccg gtagggccgg
    14881 ttggtgccgt cggcgatcag gtagacgccg aactcgccct tgggcgcctc gaccgcggta
    14941 taggtttcgc cggcgggcac gtggtagccc tcggtataga gcttgaagtg gtggatcagg
    15001 gcctccatcg agcgcttcat ctgctcgcgc ttgggcgggg cgatcttgtt gtcctcgacc
    15061 accaccgggc catcgggcat ctgatccagg cactgggcga tgatgcgcag gctctggcgc
    15121 atctcctcga gccgcaccag gtagcggtcg tagcagtcgc cgttcttgcc caccgggatg
    15181 tcgaagtcca tgcgctcgta gaccgcgtag ggctgcgcct tgcgcaggtc ccagggcacg
    15241 cccgagccgc gcagcatcgg gcccgagaag ccccagtcga gcgcctgctc agccgtgacc
    15301 acgccgatgt cgacgttgcg ctgcttccag atgcggttgc cggtcagcag ctcctcgacg
    15361 tcgtcgagca ccttggggaa cccgtccgcc caggccgcga tgtcctcggg caggcccgcc
    15421 ggcatgtcct gatgcacccc gcccgggcgg acataggccg cgtgcatgcg cgcgcccgag
    15481 acccgctcgt agaactccat cagcatctcg cgctgctcga acagccacag gaacggcgtc
    15541 atcgcgccga tgtcgaggcc gttggccgag acctgcaggc agtggttgag gatgcgggtg
    15601 atctcgtcga acagcacgcg gatgtacttg gcgcgctcgg gcacctcgat gccgagcagc
    15661 ttctcgaccg ccaggctgaa gcagtgctcc tgggccatga tcgacacgta gtcgaggcgg
    15721 tcgaaatagg gcagcgcctg caggtaggtc ttgtactcga tcagcttctc ggtgccgcgg
    15781 tgcaagaggc cgatatgcgg gtcggcgcgg tccaccacct cgccgtccat ctccagcacc
    15841 aggcgcagca cgccgtgcgc cgccggatgc tgggggccga agttgaaggt gaaggtcctg
    15901 atgtcggtgg tcgccatcag ccgcccttgg gcccctcctc ggccttctcg tcgccgggca
    15961 ggacgtagcg cgcgccttcc catgggctga tgaagtcgaa tgtgcggaac tcctgggtca
    16021 ggcgcaccgg ctcgtagacc acccgcttct gctcctcgtc gtagcgcacc tcgacatagc
    16081 cggtgagcgg gaagtccttg cgcagcgggt ggccgtcgaa gccgtagtcg gtgaggatcc
    16141 gcctgaggtc cggatggtcg gagaagaaga tgccatagag gtcccagacc tcgcgctcgt
    16201 accagccggc ggagctgaac accgcgcacg ccgagggcac gggcgcctcg tcgtcgaccg
    16261 gcaccttgac gcggacgcgc tggttctggg tgtggctcag cagatgatag acgacgtcga
    16321 agcgcggcac gcgctctggc cagtcgacgc cgcagatgtc ggtgagctgc ctgaaccggc
    16381 actccgggtc gtcgcgcagg aaagtgagca ggcgaacgac gcccgcggga gtcgatcgca
    16441 cgcacagctc gtccagcgcc acggtcgggg aggcggcgag ctcgggcacg gccgagacga
    16501 tgtagtcgcc gagatcgatg agcgcctggt tcatgctcgt ggtcggtcgg tcccgctcca
    16561 gcccgccatc agcgcgccag ggtgccggtg cggcggatct tcttttgcag ctggaggatt
    16621 ccgtacagca gcgcctcggc ggtgggcggg catccgggca catagatgtc caccggcacg
    16681 atgcggtcgc agccgcgcac caccgaatag gagtagtgat agtagccgcc gccgttggcg
    16741 cagctgccca tcgagatgac gtagcgcggc tcggccatct ggtcgtagac cttgcgca
//
LOCUS       DASWHU010000074         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_769942, whole genome shotgun sequence.
ACCESSION   DASWHU010000074 DASWHU010000000
VERSION     DASWHU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769942"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VGB88_02710"
     CDS             complement(<1..851)
                     /locus_tag="VGB88_02710"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9646382.1"
                     /translation="MAGNGRKLRSSGWFGGRDMHGFIHRSWMKNQGFGPENFDGRPVI
                     GIANSASEVTPCNAHLDRVAEAVKRGVWKAGGFPLEFPTMSLGEPVMRPTTMLFRNLM
                     AMDVEESIRANPFDGVVLLCGCDKTTPAQVMGAASVDLPAIVVTGGPMLNGKFRGRDI
                     GSGTDVWRFTDDLRAGRMSLEDYLEAESCLSRSAGHCMTMGTASTMASMVEALGLQPP
                     GGAAIPAVDSRRYALAEYAGTRIVEMVGEDLRLSKVLTRDAFENAIKVNAAIGGSTNA
                     IVHLLAIA"
     gene            complement(959..1129)
                     /locus_tag="VGB88_02715"
     CDS             complement(959..1129)
                     /locus_tag="VGB88_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646383.1"
                     /translation="MRRTESDFDDLDEPRDDGARGPRQRACLMCGRSFESEGAHNRIC
                     RRCKASQTYRSS"
     gene            1336..1932
                     /locus_tag="VGB88_02720"
     CDS             1336..1932
                     /locus_tag="VGB88_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX9646384.1"
                     /translation="MSEAPMALAGALWRVESERRLLRGAILAVAGGAFLVACSKVNVP
                     LWPVPITLQTYAVLLIGAAYGLRLAVGTILLYLAVGLAGVPVFAGPTGGPGVLLGPTG
                     GYLVGWVGAVAVVGWLAERGWTRTLPKALAALLAGNAAIYLLGLPWLALFVAPEQVLA
                     TGLTPFLVGDGAKLVLAAITSTQGWRLARGLANRPRER"
     gene            1956..3365
                     /locus_tag="VGB88_02725"
     CDS             1956..3365
                     /locus_tag="VGB88_02725"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9646385.1"
                     /translation="MADDLTFLSATDLVELYRARKLSPVEVTRAVLGRIERTREALNC
                     YVMVDADSALASARASEARWVRGEPKGLVDGVPTSIKDLVLTRGWPTLRGSLTVDPDQ
                     PWDEDAPATARLREHGAVLLGKTTTPEYGHKGVTDSPLTGITRNPWDRTRTPGGSSGG
                     ASAAVAAGLGQLAIGTDGGGSIRIPSSFAGVFGIKPSYGRVPSYPASPFGTISHVGPM
                     TRTVADAALMLTVIAGPDWRDWHALPCDGADYRHDLDRPLDGLRVAYSRTLGMPEVPL
                     EAEVGAIVDAAARRFEELGASVEEADPDWPHEPGDLFMIYWSIGAARLVSTMTADQID
                     KLDRTILTFAEAGRRYSGLDIRAAQLARGDNGLALNRFFERHQLLLSPTMPMAAFDAG
                     IAYPSEVFAGRPLRWTPYTFPINLTKHPAASIPCGFTAAGLPVGLQVIGPSFEEAVVL
                     RACRAFETANPLHRRHPEL"
     gene            3424..>4574
                     /locus_tag="VGB88_02730"
     CDS             3424..>4574
                     /locus_tag="VGB88_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX9646386.1"
                     /translation="MDMARIGATAKGGVCRLALTEVDRAGRDTFVRWAEAAGCAVTVD
                     GIGNIFARRAGRDEALAPVLVGSHLDSQPTGGKFDGAYGVLAALEVVRTLNDARLETL
                     RPIEAVSWTNEEGSRFAPAMMGSGAFAGALPLADMLARTDAEGSITVGAALASIGYDG
                     AAPAAPRPVHAYFEAHIEQGPILEAAHTTIGVVTAIQGIRWFDCRIEGMEAHAGPTPM
                     AARRDALLGAARLVERVRAIALDHAPDGRGTVGEFRVHPGSRNVIPGQVELSVDLRHP
                     DAGALAAMAAALDEAVAAVLADSGLGGALEEVWYSPPTAFDAACVDTVRRAAAEVGAA
                     HRDITSGAGHDAKYLADICPAAMVFIPCKGGISHNEIEDADPAHVAAGC"
BASE COUNT          599 a         1656 c         1654 g          665 t
ORIGIN      
        1 gcgatcgcca gcaggtggac gatggcgttg gtcgagccgc cgatcgcggc gttgaccttg
       61 atcgcgttct cgaacgcgtc gcgggtgagc accttcgaca gcctgaggtc ctcgcccacc
      121 atctcgacga tgcgcgtccc ggcatattcg gcgagcgcgt agcggcgcga atccaccgcc
      181 gggatcgccg ccccgccggg cggctgcagg cccagcgcct cgaccatgct ggccatggtc
      241 gaggcggtgc ccatggtcat gcaatggccg gcgctgcggc tgaggcacga ttcggcctcg
      301 agatagtcct cgagcgacat gcgccctgcg cgcaggtcgt cggtgaagcg ccagacgtcg
      361 gtgcccgagc cgatgtcgcg gccgcggaac ttgccgttga gcatcggccc gccggtgacc
      421 acgatggccg gcaggtcgac gctggccgcg cccatcacct gcgccggcgt ggtcttgtcg
      481 cagccgcaca gcagcaccac gccgtcgaac gggttggcgc gaatcgactc ttccacgtcc
      541 atcgccatca gattgcggaa cagcatcgtg gtcgggcgca tcaccggctc gcccagcgac
      601 atggtcggga actcgagcgg gaagccgccg gccttccaca cgccgcgctt gaccgcctcg
      661 gcgacgcggt cgagatgggc gttgcagggg gtgacctcgg acgccgagtt ggcgatgccg
      721 atcaccggcc ggccgtcgaa gttctcaggc ccgaagccct ggttcttcat ccacgagcga
      781 tggatgaagc cgtgcatgtc gcggccgccg aaccagccgg aactcctgag ctttctgccg
      841 ttgcccgcca tttctgcctc cctgccgatg ggcacgccga acggcgcgcc ggcctttcat
      901 agaccggtgc cgagggcagg taaagcgcgt cgctccgcgc ctgcgcgcgc gctcgcgctc
      961 aggagctgcg gtaggtctgg ctcgccttgc agcgccggca gatgcggttg tgggcgccct
     1021 cggattcgaa gctgcggccg cacatcaggc aggcgcgttg gcgaggcccg cgcgccccgt
     1081 cgtcgcgggg ctcgtcgagg tcgtcgaaat cgctctcggt ccgccgcacc ggcgccggct
     1141 cactcttggt ccaatcaccc atggctgccc acataatgac cgaaaatgtc aaaaaggtgg
     1201 caaaataccg gtcctggctt agcggaccgc gcatcctccc ggagcgggcc gatgatgcgt
     1261 cgtcgccgat tgccgatcgg gcgggtttgg ctcattgtgg ccgccactgg gcaacagcct
     1321 cgatgggggt ccgagatgag cgaagcaccg atggcgctgg caggcgcgct gtggcgggtc
     1381 gagtccgagc gccgcctgct gcgcggcgcg atcctggccg tggccggcgg cgccttcctg
     1441 gtcgcctgct cgaaggtcaa cgtgccgctg tggccggtgc cgatcacgct ccagacctac
     1501 gcggtgctgc tgatcggcgc ggcctacggc ctcaggctcg cggtcggcac gatcctgctc
     1561 tacctggccg tcgggctcgc cggggtgccg gtgttcgccg ggccgaccgg cgggccgggc
     1621 gtgctgctcg ggccgaccgg cggctatctg gtgggctggg tgggcgcggt cgcggtggtc
     1681 ggctggcttg ccgagcgcgg ctggacccgg accctgccga aggcgctggc ggcgctgctc
     1741 gcgggcaatg cggccatcta cctgctcggg ctgccgtggc tcgcgctgtt cgtcgcgccc
     1801 gagcaggtgc tggcgacggg cctcaccccg ttcctggtcg gcgacggggc caagctcgtg
     1861 ctcgcggcga tcacctccac ccagggctgg cggctcgccc gcgggctggc gaacaggccg
     1921 cgcgagcgct agcggacgcc atcggcggcc gggcgatggc ggatgatctg acgttcctgt
     1981 cggcgaccga tttggtcgag ctctaccggg cgcgcaagct gtcgccggtc gaggtcaccc
     2041 gggcggtgct cgggcgcatc gagcggacgc gcgaagcgct gaactgctac gtcatggtcg
     2101 acgccgacag cgcgctggcc agcgcgcggg cctccgaggc gcgctgggtg cggggcgagc
     2161 cgaaggggct ggtcgacggc gtgccgacat cgatcaagga cctggtgctc acccgcggct
     2221 ggcccacttt acgcggctcg ctgaccgtcg atcccgacca gccctgggac gaggacgcgc
     2281 cggccacggc gcggctgcgc gagcacgggg cggtgctgct cggcaagacc acgacccccg
     2341 aatacggcca caagggagtg accgacagcc cgctcaccgg catcacccgc aacccctggg
     2401 accggaccag gaccccgggc ggctccagcg gcggcgccag cgcggcggtg gcggcgggcc
     2461 tcggccagct cgccatcggc accgacggcg gcggctcgat ccgcatcccg tcgtcgttcg
     2521 ccggcgtctt cggcatcaag ccgagttacg gccgggtgcc gtcctatccg gcgagcccgt
     2581 tcggcaccat ctcccatgtc gggccgatga cccgcacggt cgccgacgcc gcgctgatgc
     2641 tgaccgtgat cgccgggccc gactggcgcg actggcatgc gctaccctgt gacggcgccg
     2701 actaccgcca cgacctcgac cgcccgctcg acggcttgcg cgtcgcctac agccgcaccc
     2761 tcggcatgcc cgaggtgccg ctcgaggccg aggtcggcgc catcgtcgat gccgccgcgc
     2821 gccggttcga agagctcggc gccagcgtcg aggaggccga ccccgactgg ccgcacgagc
     2881 ccggcgacct gttcatgatc tactggagca tcggcgccgc gcggctcgtc tcgaccatga
     2941 ccgccgacca gatcgacaag ctcgatcgga ccatcctgac cttcgccgag gccggccggc
     3001 gttacagcgg gctcgacatc cgcgcggcgc agcttgcgcg cggcgacaac ggcctggcgc
     3061 tcaaccgctt tttcgagcgc caccagctgc tgttgtcccc gaccatgccg atggcggcgt
     3121 tcgacgcggg catcgcctat ccgtccgagg tgttcgccgg ccgcccgctc aggtggacgc
     3181 cctacacctt tccgatcaac ctgaccaagc atccggcggc ctcgatcccg tgcggcttca
     3241 ccgccgccgg gctgccggtc ggcctgcagg tgatcgggcc gagcttcgag gaggcggtgg
     3301 tgctgcgcgc gtgccgcgcc ttcgagaccg ccaacccgct ccaccggcgc cacccggagc
     3361 tgtgaggtgg cggaccgggc gaacgagctc aaggtcgacg ggacgcgcct gtgggcgtcg
     3421 ctgatggaca tggcccgcat cggcgcgacg gccaagggcg gggtgtgccg gctggcgttg
     3481 accgaggtcg accgcgccgg gcgcgacacg ttcgtgcgct gggccgaggc cgcgggatgc
     3541 gcggttaccg tcgatggcat cggcaacatc ttcgcccgcc gcgcgggccg cgacgaggcg
     3601 ctcgcgccgg tgctggtcgg cagccacctc gacagccagc cgaccggggg caagttcgac
     3661 ggcgcctatg gcgtgctcgc cgcgctcgag gtggtgcgca ccctcaacga cgcgcgcctg
     3721 gagaccttgc gcccgatcga ggcggtgtcg tggaccaacg aggaagggtc gcggttcgcg
     3781 ccggcgatga tgggctcggg cgcgttcgcc ggcgccttgc cgctcgccga catgctcgcc
     3841 cgcaccgatg ccgagggcag cattacggtc ggcgccgcgc tggcctcgat cggctacgac
     3901 ggcgcggcgc ccgcagcccc gcgcccggtc cacgcctatt tcgaggccca tatcgagcag
     3961 gggccgatcc tcgaggccgc gcacaccacc atcggcgtgg tcaccgcgat ccagggcatc
     4021 cgctggttcg actgccgcat cgagggcatg gaggcccatg ccgggccgac gccgatggcg
     4081 gcgcggcgcg atgcgctcct gggcgcggcg cggctggtcg agcgggtgcg tgcgatcgcg
     4141 ctcgaccatg ccccggacgg gcgcggcacg gtcggcgagt tccgcgtcca cccgggctcg
     4201 cgcaacgtga tcccgggaca ggtcgagctc agcgtcgatc tgcgccaccc cgacgcgggc
     4261 gcgctcgccg ccatggccgc ggcgctggac gaggcggtcg ccgcggtgct tgccgactcg
     4321 ggcctcggcg gcgcgctcga ggaggtctgg tactcgccgc cgaccgcgtt cgatgcggct
     4381 tgcgtcgata cggtgcgccg ggccgcggcc gaggtcggcg ctgcgcaccg cgacatcacc
     4441 tcgggcgccg gccacgacgc caagtacctg gccgacatct gtccggcggc gatggtgttc
     4501 atcccgtgca agggcggcat cagccacaac gagatcgagg acgccgaccc cgcccacgtc
     4561 gccgccggct gcga
//
LOCUS       DASWHU010000075         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2610663, whole genome shotgun sequence.
ACCESSION   DASWHU010000075 DASWHU010000000
VERSION     DASWHU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610663"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VGB88_02735"
     CDS             complement(<1..275)
                     /locus_tag="VGB88_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646387.1"
                     /translation="MVPWLLTGIALLLALLVLARLFAAANPRTLVQVLKWSAIVLALL
                     LAVVLVFRGQAAIAAVLGGLAALLWRAARFVPPWAWFRMWQSGRGWWR"
     gene            complement(290..1000)
                     /locus_tag="VGB88_02740"
     CDS             complement(290..1000)
                     /locus_tag="VGB88_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9646388.1"
                     /translation="MASNKDKLPAPSSDAEVQAFLEQMASLPAAGPRGRMGRLIFALD
                     ATASREPTWDRACHIQAEMFTETAALGTLAVQLVYFRGFGEFDSSPFEVASEALLKRM
                     VAVFCLGGRTQIARVLRHGLRESAREKVDALVYVGDCCEESVDELCALAGELGLHGVP
                     AFMFHEGHDAIAERAYRQIARLTGGVYCRFDASSADTLRQLLSAVAVYAVGGRAALED
                     FNRRAGRAVLSLPGPRGR"
     gene            complement(1024..1872)
                     /locus_tag="VGB88_02745"
     CDS             complement(1024..1872)
                     /locus_tag="VGB88_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX9646389.1"
                     /translation="MAARVIVVGNEKGGSGKSTVAVHLALGLLAAGRSVATVDADVRQ
                     QTMTRFFARRAAYAEAHGLALATPAHRVLAAADDGDGQAAEDAVAVTVRAAAAESDVV
                     VIDCPGSDTLLARAAHAAADVLVTPVNDSFVDLDVLVEVGAEDFAAGRPSRYCEMVWE
                     QKKRRAARDRSAIDWIVIRNRLSHLDALNKRRVQAALEQLAPRVGFRVQPGLAERVIF
                     RELFPRGLSVLDGAATGRRMTMSHVAARDEIRELLGAIFRPRWLPAAPAAVDARPGTS
                     LGGANL"
     gene            2013..3215
                     /locus_tag="VGB88_02750"
     CDS             2013..3215
                     /locus_tag="VGB88_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX9646390.1"
                     /translation="MVKERVRTVDTDSLRPAPLLFTPLELRGVRLPNRIVISPMCQYS
                     AHEGFPADWHFVHLGKFAAGGAGIVFVEATAVEKRGRITHGDTGLWSDEHIAPLAHIV
                     QFLKRQGAVAAIQLAHSGRKAAMQRPWFGNGPMDDADIARGDLPWQPVGASALPVDTG
                     WQVPRALETSEVPAIVEAFAAAARRADEAGFDVVEIHSAHGYLLHSFLSPLSNRRNDA
                     YGGDRTGRMRLPLEVVEAVRAVWPEHKPLFYRISSTDGVDGGWTIEDTVALAKELKPR
                     GIDVIDCSSGGISGPATAASVKSKRAPGWQVPFAETVRREAGIKTQAVGLITHPFQAE
                     AILARGQADLIAIGREALVDAFWPLNAAAMLGADATFAEWPEQYGWWLVRRASSSEFY
                     RPEAEAAE"
BASE COUNT          481 a         1209 c         1162 g          406 t
ORIGIN      
        1 cgccaccagc cgcgccccga ctgccacatc cggaaccacg cccacggcgg cacgaagcgc
       61 gccgcccgcc acaacagcgc cgccaggccg ccgagcacgg ccgcgatcgc ggcctggccg
      121 cggaacacca gcaccaccgc gagcagcagc gcgagcacga tagcgctcca tttgagcacc
      181 tggaccaagg tccgcggatt ggcggcggcg aacaggcgcg cgagcaccag cagcgccagc
      241 aacagcgcga tgccggtcaa cagccagggg accaccggct gcgcctcctt cagcgccccc
      301 gcggcccggg caggctgagc accgcccgcc cggcacgccg gttgaagtcc tcgagcgccg
      361 cccgcccgcc gaccgcgtac accgccaccg ccgacaagag ctggcgcagc gtgtcggcgc
      421 tcgatgcatc gaaccggcag tagacgccgc cggtcagccg cgcgatctgg cggtaggcgc
      481 gctcggcgat cgcgtcatgg ccctcgtgga acatgaacgc gggcacgccg tgcaggccga
      541 gctcgccggc gagcgcgcac agctcgtcga cactctcctc gcagcagtcg ccgacataga
      601 ccagcgcatc gaccttctcg cgcgcgctct cgcgcaggcc atggcggagc acgcgcgcga
      661 tctgggtgcg cccgcccagg cagaacaccg cgaccatgcg cttgagcagc gcctcgctcg
      721 ccacctcgaa ggggctcgaa tcgaactcgc cgaagccgcg gaaatagacc agctggaccg
      781 cgagcgtgcc gagcgccgcg gtctccgtga acatctcggc ctggatgtgg cacgcccggt
      841 cccaggtcgg ctcgcggctg gcggtggcgt cgagggcgaa gatcagccgc cccatgcgcc
      901 cgcgcggccc ggccgcgggc agcgacgcca tctgctcgag gaacgcctgc acctcggcgt
      961 ccgaggacgg ggcgggcagc ttgtccttgt tgcttgccat gggctcaagc gattctccag
     1021 gccctataga ttggccccgc cgagcgaagt tccaggccgc gcatcgaccg ccgcgggggc
     1081 ggcgggcaac cagcgcggcc ggaagatcgc gcccaagagc tcgcgaatct cgtcgcgcgc
     1141 ggcgacgtgc gacatggtca tgcgccggcc ggtggcggcg ccgtcgagca cgctcagccc
     1201 gcgcgggaac agctcgcgga agatcacccg ctcggcgagc cccggctgca cgcggaagcc
     1261 gacccggggc gcgagctgct cgagcgccgc ctggacgcgg cgcttgttga gcgcgtcgag
     1321 atgcgacagc cggttgcgga tcacgatcca gtcgatggcc gagcggtcgc gcgcggcgcg
     1381 gcgcttcttc tgctcccaca ccatctcgca gtagcggctc gggcggccgg cggcgaagtc
     1441 ctcggcgccc acctcgacca gcacgtcgag gtcgacgaag ctgtcgttga ccggcgtgac
     1501 cagcacgtcg gcagcggcat gggcggcgcg cgcgagcagg gtgtcgctgc ccgggcagtc
     1561 gatcaccacc acgtcgcttt ccgccgccgc cgcgcgcacg gtcacggcga ccgcgtcctc
     1621 ggcggcttgc ccatcgccgt cgtcggcggc ggccagcacg cggtgcgcgg gcgtcgccag
     1681 ggccaggccg tgggcctcgg cataggccgc gcgccgggcg aagaaccggg tcatggtctg
     1741 ctggcggacg tcggcgtcga cggtcgccac gctccgcccg gcggcgagca ggccgagggc
     1801 gagatggacc gcgacggtcg acttgccgga gccgcccttc tcgttgccga ccacgatcac
     1861 gcgcgccgcc atggcgatgc tcctgggacg aaggcggggg tcaaagccgg gccggcgcca
     1921 gggatagcac gattcccgcc gccgcggcgc cattgacgcg attgcggccc ggcaccgcat
     1981 tcggcatagt cgggcgcgcc gatccccgat tgatggtgaa agagagagtc cgcaccgtgg
     2041 ataccgattc cttgcgccct gccccactgc tgttcacgcc gctcgagctg cgcggcgtgc
     2101 gcctgcccaa ccgcatcgtc atctcgccga tgtgccagta ctcggcccat gagggcttcc
     2161 cggccgactg gcacttcgtc catctcggca agttcgccgc cggcggcgcg gggatcgtgt
     2221 tcgtcgaggc gaccgcggtc gagaagcgcg ggcgcatcac ccacggcgat accgggctgt
     2281 ggagcgacga gcacatcgcg ccgctcgccc acatcgtcca gttcctcaag cgccagggcg
     2341 cggtggcggc gatccagctc gcccattccg ggcgcaaggc ggcgatgcag cggccgtggt
     2401 tcggcaacgg cccgatggac gacgccgaca tcgcccgcgg cgacctgccg tggcagccgg
     2461 tcggcgccag cgcgctgccc gtggacaccg gctggcaggt gccgcgcgcg ctcgagacaa
     2521 gcgaggtgcc ggcgatcgtc gaggcgttcg ccgccgccgc ccggcgcgcc gacgaggccg
     2581 ggttcgacgt ggtcgagatt cacagcgccc acggctacct gctgcacagc ttcctgagcc
     2641 cgctgtcgaa ccggcgcaac gacgcctatg gcggcgaccg caccgggcgc atgcgcctgc
     2701 cgctcgaagt ggtcgaggcg gtgcgcgccg tctggcccga gcacaagccg ctgttctacc
     2761 gcatctcgtc gaccgacggc gtcgacggcg gctggacgat cgaggacacg gtggcgctgg
     2821 cgaaagagct caagccgcgc ggcatcgacg tgatcgactg ctcgtcgggc ggcatctccg
     2881 ggccggcgac cgcggcctcg gtcaagagca agcgcgcgcc cggctggcag gtgccgtttg
     2941 ccgagaccgt gcgccgcgag gcgggcatca agacccaggc ggtcgggctg atcacccatc
     3001 cgttccaggc cgaggcgatc ctcgcccgtg gccaggctga cctgatcgcg atcgggcgcg
     3061 aggcactggt cgacgcattc tggccgctca acgcggcggc gatgctgggc gccgacgcca
     3121 ccttcgccga gtggcccgag cagtacggct ggtggctggt gcgccgcgcc tcgtcgagcg
     3181 agttctaccg gcccgaggcc gaagccgcgg agtgagggcg cgtaaatgaa ccggctgccg
     3241 tccttcgaga cgctcgct
//
LOCUS       DASWHU010000076         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2611904, whole genome shotgun sequence.
ACCESSION   DASWHU010000076 DASWHU010000000
VERSION     DASWHU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611904"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VGB88_02755"
     CDS             complement(<1..654)
                     /locus_tag="VGB88_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9646391.1"
                     /translation="MTGGACRLERGFTLLELLVAMTLLGLMLALLFGGLRTGTQVWRA
                     GDQRSDALARIEAVHGFLRRQVGALYPMAEPGDAGEAPEIAFEGEAERLRFVGMLPAH
                     FGAGGFQTVEVATDEHDGRRDLRVAWWPYRPGERAPEQIDPDSTSVLVEDVEEVEFGY
                     FGTTDQRSPPDWTDRWLERSEPPDLVRLRLSFGAGDARYWPELVIHPAIDRTVPAGTP
                     "
     gene            complement(651..1124)
                     /locus_tag="VGB88_02760"
     CDS             complement(651..1124)
                     /locus_tag="VGB88_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9646392.1"
                     /translation="MGKATMSCSTGSPVRSRSSSNARRAPRCSRGFTLIEIVVALVVL
                     MIGLGTLLRLFSTGMGATAAAESRALAVLLAQSRLAAIGIETPLEAGIEDGAFDDRFR
                     WTAEITPIEEAGEAQDLGTGFTLWRVAVTVSWGAAPRGGSVTLTSARLAAAEDAQ"
     gene            complement(1063..1500)
                     /locus_tag="VGB88_02765"
     CDS             complement(1063..1500)
                     /locus_tag="VGB88_02765"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEX9646393.1"
                     /translation="MAQRGFTLIELLVVLVVIGLILAAAPVALRRGAPAIEARVAAGE
                     VAAVLRRARSEAILGNRDVAVAFDTDAGAYALGEDAPWQALPGRVTLSVETARSELRD
                     AHVAGVRFFADGSATGGRITVARDGQSYHVMLDWLTGQVSVIE"
     gene            complement(1511..1966)
                     /gene="gspG"
                     /locus_tag="VGB88_02770"
     CDS             complement(1511..1966)
                     /gene="gspG"
                     /locus_tag="VGB88_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019570495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX9646394.1"
                     /translation="MTTLAQRRRGPRARGTRRDGGFTLLELLVVLAILALIATFAAPR
                     VLKYLGGARTDSARLQIESLATVLDLYRLEVGHYPSEDEGIEALIERPSGVEKWNGPY
                     VRKREQLIDPWGRAFEYHYPGEHAEYDLYSLGADGQDGGEGEDQDVTSW"
BASE COUNT          296 a          769 c          695 g          255 t
ORIGIN      
        1 cggggtgccg gccggcacgg tgcggtcgat cgccgggtgg atgaccagct cgggccaata
       61 gcgcgcatcg cccgccccga agctcagcct gaggcgcacc aggtccggag gctcgctccg
      121 ctccagccag cggtcggtcc agtccggcgg cgagcgctgg tcggtggtgc cgaaatagcc
      181 gaactcgacc tcttcgacgt cctcgaccag caccgacgtc gagtccgggt cgatctgctc
      241 cggcgcgcgc tcgccgggcc ggtacggcca ccacgccacc ctgaggtcgc gccgcccgtc
      301 gtgctcgtcc gtcgcaacct cgacggtctg gaagccgccg gcgccgaaat gggccggcag
      361 catgccgacg aagcggagcc gctcggcctc gccttcgaag gcgatctcgg gcgcctcgcc
      421 ggcgtcgccc ggctcggcca tcggatagag cgcacccacc tggcggcgca ggaagccgtg
      481 caccgcctcg atccgggcga gcgcgtccga gcgctgatcc cccgctcgcc acacctgcgt
      541 gccggtgcgc aggccgccga acaggagcgc cagcatcagc ccgagcaggg tcatcgccac
      601 caacagctcg agcaaggtga agccgcgctc caggcgacac gcgccgccgg tcattgggcg
      661 tcctcggcgg cggcgagacg ggcgctggtc aaggtcaccg agccgccgcg cggcgcagct
      721 ccccacgaca ccgtgaccgc gacccgccac aaggtgaatc cggtgccgag gtcctgcgcc
      781 tcgcccgcct cctcgatcgg cgtgatctcg gccgtccagc ggaagcggtc gtcgaacgcg
      841 ccgtcctcga tcccggcctc gagcggcgtc tcgatgccga tcgcggcgag gcgcgactgc
      901 gcgagcagca ccgccagcgc ccgcgactcg gcagccgcgg tcgcgcccat gccggtcgag
      961 aacagccgca gcagcgtgcc gagcccgatc atcagcacca ccagcgcgac cacgatctcg
     1021 atcagcgtga agccgcggct gcatcgcggc gcccgccgtg cgttactcga tgaccgagac
     1081 ctgaccggtg agccagtcga gcatgacatg gtagctttgc ccatcgcgcg cgaccgtgat
     1141 gcgcccgccg gtggcgctgc cgtcggcgaa gaaccgcacg ccggcgacgt gcgcgtcgcg
     1201 cagctcggag cgcgcggtct cgaccgacag cgtcacccgt ccgggcagcg cctgccacgg
     1261 cgcgtcctcg ccgagcgcat aggcgccggc atcggtgtcg aaggcgacgg cgacgtcgcg
     1321 gttgccgaga atcgcctcgc tgcgtgcgcg ccgcagcacc gcggcgacct cgcccgccgc
     1381 aacccgcgcc tcgatcgccg gcgcgccgcg cctgagcgcc accggcgccg ccgccaggat
     1441 cagtccgatc accaccagca ccaccagcag ctcgatcagc gtgaagccgc gctgggccac
     1501 cggcgcccgc ctaccagctg gtgacgtcct ggtcctcgcc ctcgccgccg tcctggccgt
     1561 cggcgccgag cgagtagagg tcgtactcgg cgtgctcgcc gggatagtgg tactcgaagg
     1621 cgcgccccca cgggtcgatc agctgctcgc gcttgcgcac gtaggggccg ttccatttct
     1681 cgaccccgga cggccgctcg atcagtgcct cgatgccttc gtcctcgctc ggataatggc
     1741 cgacctcgag acggtagagg tcgagcacgg tggccaggct ctcgatctgc aggcgggccg
     1801 agtcggtgcg cgccccgccc agatatttga gcacccgcgg cgcggcgaag gtcgcgatca
     1861 gggcgaggat cgccagcacc accagcagct cgagcagcgt gaagccgccg tcacgccggg
     1921 tgccgcgggc gcgcggacca cgacggcgtt gcgccagggt cgtcacagcg ggatgtcgta
     1981 gacgctgaac atggcgctca ggatcgagcc gatga
//
LOCUS       DASWHU010000077         6002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_27753, whole genome shotgun sequence.
ACCESSION   DASWHU010000077 DASWHU010000000
VERSION     DASWHU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27753"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VGB88_02775"
     CDS             <1..302
                     /locus_tag="VGB88_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEX9646395.1"
                     /translation="TARAAKVILDRLGAREVVLVTTPAHIARMAAALRHAGLVVRALP
                     ARAAAPGRPAFGVLTEFIPSAGGLAQSANAVREYVAILWYLGQGYFRPSDLWADG"
     gene            334..834
                     /locus_tag="VGB88_02780"
     CDS             334..834
                     /locus_tag="VGB88_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX9646396.1"
                     /translation="MRHWYAVFARAHAEQRAELNLGRQRFEVWLPRYRKMRRHAGRSE
                     QVLRPLFPRYLFVALDVAHERWRAVLSTYGVAGLVCSGEAPVAVPEAVIEGLRARADA
                     DGTFTLERAARLRRGDRVRIEAGPMRDLEGIFEAATDAERVVVLLELMGRGVRVTVPA
                     EHVERA"
     gene            827..2062
                     /locus_tag="VGB88_02785"
     CDS             827..2062
                     /locus_tag="VGB88_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9646397.1"
                     /translation="MPDPGPAPPAPELKRGLNLALLTLYGLGTTIGAGIYVLVGEVAA
                     VAGALAPASFLIAACLAATTGLSFGELGARYPKSAGEAVYVDAGFGLAWLAALVGLMV
                     AGAGVISSAAIANGFVGYARQFVDAPPWALMSAMVAALAAAAVWGIVESVLMAALVTV
                     IEIAGLVLVVVVGLASIDVGEAAAAAAAGLDASLVPGAFAGAVLAFYAFLGFEDMVNV
                     AEEVQAPERTLPRAIVLTLAVTGVLYVAVAVAAVLVVPRADLAGAEAPLALVWQHATG
                     REPALISAIGALAVVNGALIQIIMAARVVYGLASQGWLPAVLGRVQPRTRTPALATLV
                     VASLVLALALALPLVRLAEATAFVILIVFALVNLALLRVKRRAPLPPGARAVPRAVPA
                     AGFAVSAGVALFQAWRYVA"
     gene            complement(2016..2837)
                     /gene="cysQ"
                     /locus_tag="VGB88_02790"
     CDS             complement(2016..2837)
                     /gene="cysQ"
                     /locus_tag="VGB88_02790"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9646398.1"
                     /translation="MTGPQRPAPAALADQVAEIARAAGAVILAVRARGPAVRTKADRT
                     PVTDADEAANALISARLAALTPDVPIVAEESAGGAPVPGARFWLVDPLDGTREFIAGR
                     DEFTVNIALIEDGQPVLGVVALPAQDELYVGVAGAGAWRRRGDGAPAPIRARAVPAQG
                     ALALASRSHGSARTDRWLARAGVGRIERAGSALKFCLIAAGEADLYPRFGRTMEWDTA
                     AGHAVLAAAGGSVRTVSGPDLAYGKPGFANPDFIARGADALRPRSARPGTGRRRR"
     gene            complement(2834..4714)
                     /gene="cysC"
                     /locus_tag="VGB88_02795"
     CDS             complement(2834..4714)
                     /gene="cysC"
                     /locus_tag="VGB88_02795"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX9646399.1"
                     /translation="MPQEPRERLSVVIVGHVDHGKSTLVGRLFHDTGSLPEGRLEAIR
                     EMCARRGMPFEWAFLTDALQGERDQGVTIDTAYIWFRTGKRDYVLIDAPGHKEFLRNM
                     VTGAAQADAALLLIDAAEGVREQSRRHGHLLHLLGIAQVIVVINKIDRVAYARERILA
                     IEAEYARFLSELGVVPRFVIPISAREGDNVAALSGRTPWYEGPSVLEALDHFEPSPAP
                     VDMALRLPVQDVYKFDDRRIIAGRVETGLLRTGDLVLFSPSNKTAHVASLEAWHAEPA
                     MEARAGQSVGLTLDEPLFVERGEVISHTDSPPIESDVFRARLFWLGRTPLRAGASYKL
                     KLATVEAGVTVQSIESVTEASTLSAASTDEVGQNAVAEAVLRADRMIALDEYATNPAL
                     GRCVLVEATQIVGGGTLNMAGYADQRALVSVRSTNITAHPHAVGAGERVARNRHRGGV
                     LWFTGLSGAGKSTLAGAVERALFERGFQVYVLDGDNVRAGLNANLGFAPEERAENIRR
                     VGEAAALFGDAGMVVITAFISPYRSDRTRARSAAERMLGPEAFYEIFVKADLATCEAR
                     DPKGLYARARRGEIAEFTGVSAPYEEPERPDLVVDTATAPVEACVERIVGFVTGRFAR
                     RP"
     gene            complement(4738..5532)
                     /gene="cysD"
                     /locus_tag="VGB88_02800"
     CDS             complement(4738..5532)
                     /gene="cysD"
                     /locus_tag="VGB88_02800"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9646400.1"
                     /translation="MTPLDRLESQSIYILREAFNRIDRLAMLWSLGKDSNVMVWLAKK
                     AFFGHVPFPVAHVDTGRKFPEMYAFRDRYAAEWGLDLVVGECPPIEDIDPSLPPAARA
                     AARKTAGLKALIAAHGYSGIIAGIRRDEEGTRAKERVFSPRGPGARWDVRNQPPEFWD
                     QYNTDFPPGTHVRIHPLLHWTEIDVWRYIARERIPVVELYFARDGKRYRSLGDSDITN
                     PIASTAATVDEIIAELMTTRAPERAGRAMDHEAEDSFERLRSDGYM"
     gene            5660..>6002
                     /locus_tag="VGB88_02805"
     CDS             5660..>6002
                     /locus_tag="VGB88_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646401.1"
                     /translation="MTGAGEQSDAGRRLEDRPALLAAMLADSQSAGPLWRPGAYWRPY
                     CARITRELARTGLSDVRTNQRLLKGYALGGVPAPTLPRAAWKRAVWTLAERAPVVALI
                     VGEYRRLVAAEH"
BASE COUNT          721 a         2133 c         2311 g          837 t
ORIGIN      
        1 agaccgcgcg cgcggccaag gtgatcctcg accggctggg cgcgcgcgag gtggtgctgg
       61 tgaccacgcc ggcgcacatc gcgcgcatgg cggcggcgct gcgccacgcc ggcctcgttg
      121 tgcgcgcgtt gccggcgcgc gcagcggcgc ctgggcgccc tgccttcggc gtgctcaccg
      181 agttcatccc gagcgccggc ggcctcgccc agagcgccaa cgcggtgcgc gagtacgtgg
      241 ccatcctgtg gtatctaggg caaggctatt tccgtccgtc cgacctgtgg gccgacggct
      301 gagcgcgacc aaggccggag tggacctcgc atcgtgagac attggtacgc cgtcttcgcc
      361 cgggcccatg ccgagcagcg ggccgagctc aacctggggc gccagcggtt cgaggtctgg
      421 ctgccgcggt accgcaagat gcgccgccat gccgggcgca gcgagcaagt gctcaggccg
      481 ctgttcccgc gctacctgtt cgtcgccctc gacgtggccc atgagcgctg gcgggcggtc
      541 ctgtcgacct atggcgtcgc cgggctggtg tgctcgggcg aggcgccggt ggcggtgccg
      601 gaagcggtga tcgagggcct gcgcgcgcgc gccgatgccg acggcacctt caccctggag
      661 cgcgccgccc ggctcaggcg cggcgaccgg gtgcggatcg aggccgggcc gatgcgcgac
      721 ctggaaggca tcttcgaggc ggcgaccgac gccgagcggg tggtggtgct gctcgagctg
      781 atgggccgcg gcgtgcgcgt gaccgtgccg gccgagcacg tcgagcgtgc ctgacccggg
      841 gccggcgccg ccggcgccgg agctgaagcg cgggctcaac ctggcgctgc tcaccctcta
      901 cggcctcggc accaccatcg gcgcgggcat ctacgtgctg gtcggcgagg tggcggcggt
      961 cgcgggcgcg ctggcgccgg cctcgttcct gatcgccgcc tgcctggcgg cgaccaccgg
     1021 cctcagcttc ggcgagctcg gcgcgcgcta cccgaaaagc gccggcgagg cggtctacgt
     1081 cgacgccggc ttcgggctgg cgtggctcgc ggcgctggtc gggctgatgg tcgccggcgc
     1141 cggcgtcatc tcctcggccg cgatcgccaa cggcttcgtc ggctatgcgc gccagttcgt
     1201 ggatgcgccg ccgtgggcgc tgatgtcagc gatggtggcc gccctcgccg ccgccgcggt
     1261 ctggggcatc gtcgagtcgg tgctgatggc ggcgctggtg acggtgatcg agatcgccgg
     1321 cctggtgctg gtggtcgtgg tcgggctggc cagcatcgat gtcggcgagg ccgcggcggc
     1381 ggcggcggcc gggctcgatg cctcgctggt gccgggcgcc ttcgccggcg ccgtgctcgc
     1441 cttctacgcc ttcctcggct tcgaggacat ggtcaacgtc gccgaggagg tgcaggcgcc
     1501 cgagcgcacc ttgccgcgcg cgatcgtgct gaccctggcg gtgaccgggg tgctctacgt
     1561 cgcggtcgcc gtggccgcgg tgctggtggt gccgcgcgcc gatctcgccg gcgccgaggc
     1621 gccgctggcg ctggtctggc agcacgccac cggccgcgag cctgcgctga tcagcgcgat
     1681 cggcgcgctg gcggtggtca acggcgcgct gatccagatc atcatggccg cgcgcgtggt
     1741 ctacggcctg gcaagccagg gctggctgcc ggcggtgctg ggccgggtgc agccgcgcac
     1801 gcgcacgccg gcgctcgcga ccctggtggt agcgtcgctg gtgctcgcgc tggcgctggc
     1861 gctgccgctg gtgcggctcg ccgaggcgac cgcgttcgtc atcctgatcg tgttcgccct
     1921 ggtcaacctg gcgctgttgc gggtgaagcg gcgcgcgccg ttgccgccgg gcgcccgggc
     1981 ggtgccgcgg gcggtgccgg cggcgggatt cgcggtcagc gccggcgtcg ccctgttcca
     2041 ggcctggcgc tacgtggcct gagcgcgtcc gcgccgcggg cgatgaagtc ggggttggcg
     2101 aagccgggct tgccgtaggc gaggtccggg cccgagaccg tgcgcacgct gccccccgcg
     2161 gcggcgagca cggcgtggcc ggcggccgtg tcccactcca tggtccgccc gaaacgcggg
     2221 tagaggtccg cctcgccggc ggcgatcagg cagaacttga gcgccgagcc ggcgcgctcg
     2281 atgcgcccga ccccggcgcg cgccagccac cggtcggtgc gcgcgctgcc gtggctgcgg
     2341 ctggcgagcg cgagggcgcc ctgcgccggc accgcccgcg cgcggattgg cgcgggcgcg
     2401 ccgtcgcccc gccggcgcca ggcgccggcg ccggcgacgc cgacatagag ctcgtcctgc
     2461 gccggcaggg cgaccacgcc gagcaccggc tggccgtcct cgatcagggc gatgttgacc
     2521 gtgaactcgt cgcgcccggc gatgaactcg cgcgtgccgt cgagcgggtc gaccagccag
     2581 aagcgcgcgc caggcaccgg cgccccgccc gcgctttcct cggcaacgat cgggacatcg
     2641 ggggtgagcg cggcgaggcg cgccgagatc agcgcgttgg ccgcctcgtc ggcgtcggtg
     2701 accggggtgc ggtcggcctt ggtgcgcacc gcagggccgc gcgcgcgcac cgcgaggatc
     2761 acggcgccgg cggcgcgcgc gatctcggcc acctggtccg ccagcgccgc gggcgcgggg
     2821 cgctgcgggc cggtcatggc cggcgcgcga agcggccggt gacgaagccg acgatgcgct
     2881 cgacgcaggc ctcgaccggg gcggtcgcgg tgtcgaccac caggtcgggg cgctcaggct
     2941 cctcgtaagg cgccgagacg ccggtgaact cggcgatctc gccgcggcgc gcgcgcgcat
     3001 acaggccctt ggggtcgcgc gcctcgcagg tggcgaggtc ggccttgacg aagatctcgt
     3061 agaacgcctc gggccccagc atgcgctcgg ccgcactgcg ggcgcgggtg cggtcggagc
     3121 ggtagggcga gatgaaggcg gtgatcacca ccatgccggc gtcgccgaac agcgccgccg
     3181 cctcgccgac ccggcggatg ttctcggcgc gctcctcggg cgcaaacccg agattggcat
     3241 tcagcccggc gcgcacgttg tcgccgtcga gcacgtagac ctggaagccg cgctcgaaca
     3301 gcgcccgctc gaccgcgccg gcgagcgtcg acttgcccgc ccccgacagg ccggtgaacc
     3361 acagcacgcc gccgcggtgg cggttgcgcg cgacgcgctc gcccgcaccg accgcgtgcg
     3421 gatgcgcggt gatgttggtc gagcgcaccg acaccagcgc gcgctggtcg gcgtagcccg
     3481 ccatgttgag ggtgccgccg ccgacgatct gggtggcctc gaccagcacg cagcgcccga
     3541 gcgcggggtt ggtcgcgtac tcgtcgagcg cgatcatgcg gtcggcgcgc agcaccgcct
     3601 cggcgaccgc gttctggccg acctcatcgg tcgaggcggc gctcagggtc gaggcctcgg
     3661 tcaccgactc gatcgactgc acggtgacgc cggcctcgac ggtcgcgagc ttgagcttgt
     3721 agctggcgcc cgcgcgcagc ggcgtgcgcc cgagccagaa caggcgggcg cggaacacgt
     3781 cgctctcgat cggcggcgag tcggtgtggc tgatcacctc gccgcgctcg acgaacagcg
     3841 gctcatcgag ggtgagcccg accgactggc cggcgcgcgc ctccatcgcc ggctcggcgt
     3901 gccaggcctc gagcgaggcg acgtgcgcgg tcttgttcga cggcgagaac agcaccaggt
     3961 cgccggtgcg caacagcccg gtctcgacgc gcccggcgat gatccgccgg tcgtcgaact
     4021 tgtagacgtc ctggaccggc agcctgagcg ccatgtcgac cggcgccggc gagggctcga
     4081 aatggtcgag cgcctcgagc acgctcgggc cctcgtacca cggcgtgcgc ccggacagcg
     4141 cggcgacgtt gtcgccctcg cgcgcggaga tcgggatcac gaacctcggc accacgccga
     4201 gctcgctcag gaagcgcgcg tactcggcct cgatcgccag gatgcgctcg cgcgcatagg
     4261 ccacgcggtc gatcttgttg atcaccacga tcacctgggc gatgcccagc aggtgcagca
     4321 ggtggccgtg gcgccgcgac tgctcgcgca ccccttcggc ggcgtcgatc aagagcagcg
     4381 ccgcgtccgc ctgggcggcg ccggtgacca tgttgcgcag gaactccttg tgacccggcg
     4441 cgtcgatcag cacgtagtcg cgcttgcccg tgcggaacca gatataagcg gtgtcgatgg
     4501 tcacgccctg gtcgcgctcg ccttgcagcg cgtcggtcag gaacgcccac tcgaacggca
     4561 tgccgcggcg ggcgcacatc tcgcggatcg cctcgaggcg gccctcgggc aggctgccgg
     4621 tgtcgtggaa cagccgcccg accagggtcg acttgccgtg gtcgacatgg ccgacgatga
     4681 ccacggacag gcgttcgcgc ggctcttgcg gcatcgcggc agcgggcgtg gacgtgctca
     4741 catgtagccg tccgagcgca ggcgctcgaa cgagtcctcg gcctcgtggt ccatggcgcg
     4801 acccgcgcgc tcgggcgcgc gggtggtcat cagctcggcg atgatctcgt cgacggtggc
     4861 ggcggtgctg gcgatcgggt tggtgatatc cgagtcgccg agcgagcggt agcgcttgcc
     4921 gtcgcgcgcg aagtagagct cgaccaccgg gatgcgctcg cgcgcgatgt agcgccagac
     4981 gtcgatctcg gtccagtgca gcagcggatg gatgcgcaca tgggtgccgg gcgggaagtc
     5041 ggtgttgtac tggtcccaga actcgggcgg ctgattgcgc acgtcccagc gcgcgcccgg
     5101 cccgcgcggg ctgaacaccc gctctttcgc ccgcgtgccc tcctcgtcgc ggcggatgcc
     5161 ggcgatgatc ccgctatagc cgtgggcggc gatcagcgct ttcaggcccg cggtcttgcg
     5221 cgcggcggcg cgcgccgccg gcggcaagga ggggtcgatg tcctcgatcg gcgggcactc
     5281 gccgaccacc aggtcgaggc cccactcggc ggcgtagcgg tcgcggaagg cgtacatctc
     5341 ggggaacttg cgcccggtat cgacgtgcgc caccgggaac ggcacgtggc cgaagaacgc
     5401 cttcttggcc agccacacca tgacgttcga gtccttgccc agcgaccaca gcatcgccag
     5461 ccggtcgatg cggttgaacg cctcgcgcag gatatagatg ctctggctct ccaggcggtc
     5521 gagcggggtc atgccggggc cgggatcaag gggcaagaag ggaaccggga cgcgcgccgg
     5581 ctgaatacgg gaatttgcca gtcccggcaa cagcgtcctg cgctatcttg cgcaccggcg
     5641 cagggcgaag ggggcggcgg tgacgggggc aggcgagcaa tcggacgcgg ggcgccggct
     5701 cgaggaccgg cccgccctgc ttgccgcgat gctcgccgac tcgcagtcgg ccgggccgct
     5761 gtggcggccg ggcgcgtact ggcggcccta ctgcgcccgg atcacgcgcg agctcgcccg
     5821 cacgggcctc agcgacgtgc gcaccaacca gcgcctgctc aagggctacg cgctcggcgg
     5881 ggtgccggcg ccgaccttgc cgcgcgccgc ctggaagcgc gccgtgtgga ccctggccga
     5941 gcgcgcgccg gtggtcgccc tgatcgtcgg cgagtaccgg cggctggtgg cggccgagca
     6001 cg
//
LOCUS       DASWHU010000078         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1883253, whole genome shotgun sequence.
ACCESSION   DASWHU010000078 DASWHU010000000
VERSION     DASWHU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883253"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGB88_02810"
     CDS             complement(<1..383)
                     /locus_tag="VGB88_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9646402.1"
                     /translation="MPEVIFNGPEGRLEGRYHHGTGTSPPIAIVLHPHPQRGGTMNNK
                     VVYTLYHAFAQMGHSVLRFNFRGVGRSQGRYDHGQGELSDAASALDWLQSYNPDARAC
                     WIAGFSFGAWIALQLLMRRPEINGF"
     gene            600..1331
                     /gene="cysE"
                     /locus_tag="VGB88_02815"
     CDS             600..1331
                     /gene="cysE"
                     /locus_tag="VGB88_02815"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX9646403.1"
                     /translation="MFKRLGEDITSIIARDPAARSRLEVVLCYPGFHAVIYHRVAHAL
                     WQAGLKLAGRFVSHIGRFLTGIEIHPGAKIGRRLFIDHGMGVVIGGTAEIGDDVTLYH
                     GVTLGGTSLERGVKRHPTLEDGVIVGSGANVLGPITIGAGARIGANAVVVAPVHPGAT
                     MVGVPAKQVAQRKGKAKEKFLAYGTPDTIPDPVQRALDGLLDQVSTLTARLQELERQL
                     GERGELPAGSAEAEAGDLRRRTGSG"
     gene            1372..1833
                     /locus_tag="VGB88_02820"
     CDS             1372..1833
                     /locus_tag="VGB88_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9646404.1"
                     /translation="MRLSTKARYAVMAMVDLAQRGGARPVCLSDIARRQEISLPYLEQ
                     LFAKLRRGALVRSVRGPGGGYLLARRAEEVRIADIVVAVNGPIRATRCPPDSSSGCMG
                     DSSRCLTHDLWAELGNQIHLYLSSVSLADVCARRVLGTSMLRRPADLAAAE"
BASE COUNT          293 a          689 c          705 g          266 t
ORIGIN      
        1 atgaagccgt tgatctcggg ccggcgcatc agcagctgga gcgcgatcca ggcgccgaac
       61 gagaagccgg caatccagca cgcgcgcgcg tccgggttgt aggactgcag ccagtcgagc
      121 gccgaggcgg catcgctcag ctcgccctgg ccgtggtcgt agcggccctg gctgcgcccc
      181 accccgcgga agttgaagcg cagcaccgag tgccccatct gcgcgaatgc gtggtagagg
      241 gtgtagacca ccttgttgtt catagtgccg ccgcgctggg gatggggatg cagcacgatc
      301 gcgatcggcg gcgaggtgcc ggtaccgtga tggtagcgcc cctcgagccg cccctcgggg
      361 ccgttgaaga tcacctcggg cacggcattc ctccgccggc ggtcacgggc acggcccaat
      421 aacccagtcc ggagggcgga gataacgggc cggctctccg atcggggacg ggcaaagctt
      481 gaccaaaata ctcggatttt ctatacccag gacacgggat cgcgccctgc gccgccccgc
      541 gaccccggat cgcgcgcccg ggctcggcag cgagcaagga taacataacc ggttcagcca
      601 tgttcaagcg acttggagag gacatcacca gcatcatcgc ccgcgacccg gcggcccggt
      661 cgcggctcga ggtggtgcta tgttatccgg gtttccatgc ggtcatctat caccgcgtcg
      721 cccacgcgct gtggcaggcg ggcctcaagc tcgccggccg gttcgtgtcg cacatcgggc
      781 ggttcctgac cggcatcgag atccaccccg gggccaagat cggccggcgg ctgttcatcg
      841 accacggcat gggcgtggtc atcggcggca ccgccgagat cggcgacgac gtcaccctct
      901 atcacggggt gaccctgggc ggcacctcgc tggagcgcgg ggtgaagcgc cacccgacgc
      961 tcgaggacgg ggtgatcgtc ggctccggcg ccaacgtgct cggcccgatc acgatcggcg
     1021 ccggcgcgcg catcggcgcc aacgccgtgg tcgtcgcccc ggtgcacccc ggcgcgacca
     1081 tggtgggcgt gccggccaag caggtcgcgc agcgtaaggg caaggccaag gagaagttcc
     1141 tggcctacgg cacgcccgac acgattccgg acccggtgca gcgcgcgctc gacggcctgc
     1201 tcgatcaggt cagcaccctg accgcgcgcc tccaggagct cgagcgccag ctcggcgagc
     1261 ggggcgagct gccggccggg tccgcggagg cggaggcggg cgatctcagg cgccggaccg
     1321 gcagcggctg agccgcgccc gggcccgcag cggcaacaag gatatttggc cgtgcgattg
     1381 agcaccaagg cgcgctacgc ggtgatggcg atggtcgacc tcgcccagcg cggcggcgca
     1441 cggccggtgt gcctgtccga catcgcccgc cgccaggaga tctcgctgcc ctatctggag
     1501 cagctgttcg ccaagctgcg ccgcggcgcg ctggtgcgca gcgtgcgcgg gcccggcggc
     1561 ggctacctgc tggcgcgccg ggcggaggag gtgcgcatcg ccgacatcgt agtcgcggtc
     1621 aacgggccga tccgcgccac ccgatgtcca ccggactcct cgagcggctg catgggcgat
     1681 tccagccgct gcctgaccca cgacctgtgg gcggagctcg gcaaccagat tcatctctat
     1741 ctgagctcgg tctcgctcgc cgacgtgtgc gcgcggcggg tgctcgggac cagcatgctg
     1801 cgccggccgg cggacctggc cgcggcggag tgagcggccg gcgacggccg cgcccgcgga
     1861 cgccatgacc acgacgatca gctactgcga ctacaacgcc agcgcgccgg tgcgccccga
     1921 ggcggcggcg gcggtggccg cggcgctcgc cgc
//
LOCUS       DASWHU010000079         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_29190, whole genome shotgun sequence.
ACCESSION   DASWHU010000079 DASWHU010000000
VERSION     DASWHU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29190"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGB88_02825"
     CDS             <1..351
                     /locus_tag="VGB88_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646405.1"
                     /translation="AVYASIIVAEERYLHDRFGAEYAAYCARVPRLWPDFSLYRAAAR
                     DMTFNWRRAIQKDYSSIGAWIIAFVAIEAVEDWLSKAGGFAVTDLAGPGAAILAVLLV
                     LLAVRTWKKRDPAR"
     gene            complement(569..1075)
                     /locus_tag="VGB88_02830"
     CDS             complement(569..1075)
                     /locus_tag="VGB88_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9646406.1"
                     /translation="MAETHPGPVVVCGPDRGQSYWQPVPANGYSEVKVSKHDSPHIRG
                     FSSGIQVIAPGGHVREHRHGGEDELLFFFEGRGRVVINGVAHPVEPGTSAYLGRWNRH
                     TIINDGTGELKMMWVLMPGGLEDFFAAIGRARTPGEPAPAPFPRPDNVAEIERRTVFA
                     ALDTSPQD"
     gene            complement(1175..1492)
                     /locus_tag="VGB88_02835"
     CDS             complement(1175..1492)
                     /locus_tag="VGB88_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646407.1"
                     /translation="MTHCLKAKAAVAAVLLMAAGGGAAQAEPDVSLCVPHDEAVAKLD
                     HRYGERQVGIGLEQQGETVIELFVAATGTWTVLVTRTDGLSCVAAAGESWMTTPKFAG
                     DPT"
     gene            1735..2781
                     /locus_tag="VGB88_02840"
     CDS             1735..2781
                     /locus_tag="VGB88_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HEX9646408.1"
                     /translation="MPEARALPLLPTTVVGSYPQPDWLIDRDALSHRSPPRVRAAGLW
                     RVAEPWLAEAQDDATRLAIADMERAGIDIITDGEIRRESYSNRFATALGGIDPARAGT
                     AQSRRGRPEPVPLVSGPIRRLRPVETADVAFLRANTARTIKATLPGPFTLAQQAEDAY
                     YRDPERLAMAYAEAVNAELLDLFAAGADIVQLDEPYLQARPEAARAFAVAAIDRALAG
                     APGTTALHLCMGYAALVRDKPPDYAFLAELADSAVDQVSVEAAQPHVDVARFADLGAK
                     TVILGVLDLADAAVETPETVAARIERALDFVPAERLVPAPDCGMKYLPRAVALGKLGA
                     LAAGTAMVRARLAG"
     gene            complement(2789..3175)
                     /locus_tag="VGB88_02845"
     CDS             complement(2789..3175)
                     /locus_tag="VGB88_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646409.1"
                     /translation="MTNIQAILAGAALIAAAALLGGGPAPQAAGTDGRFALAVAASHP
                     IAFVTDTATGETMVCSQSGCRRLEIAEASSVPGIDPARAQPGPPAQPSTLDTILQGPP
                     PAELQLPPPSTAPGGGSALDQMRGNF"
BASE COUNT          441 a         1249 c         1213 g          484 t
ORIGIN      
        1 gccgtctacg cctcgatcat cgtcgccgag gagcgctacc tgcacgacag gttcggcgcc
       61 gagtacgccg cctattgcgc gcgcgtgccc cgcctgtggc ccgacttctc gctgtaccgg
      121 gcggcggcgc gcgacatgac cttcaactgg cggcgcgcga tccagaagga ctactcgagc
      181 atcggcgcct ggatcatcgc cttcgtcgcc atcgaggcgg tcgaggactg gctcagcaag
      241 gcgggcggct tcgccgtaac cgacctcgcc ggaccggggg cggcgatcct tgccgtcctg
      301 ctggtcctgc tcgccgtccg cacctggaag aagcgcgacc cggcccgctg acccgcaggg
      361 cgcgctagag cgggatgata ttaagtcgga tccctgagcc tgttttggtc gtcaccccgg
      421 aatccgcgaa gcggatatcc ggggtccata cgcctttgcc acgcgctcga ccacccgtgc
      481 aagcggcggc atggatcccg gctcgcggcg ctgcgcgcct tggccggggt gacgggtgat
      541 ggatgaccat gtttcaaatc atcaggctct agtcttgggg gctggtgtcg agggcggcga
      601 acacggttcg gcgctcgatc tcggcgacgt tgtcggggcg cgggaacggc gccggcgcag
      661 gctcgcccgg agtgcgcgca cgcccgatcg cggcgaagaa gtcctcgagc ccgcccggca
      721 tcagcaccca catcatctta agctcgccgg tgccgtcgtt gatgatcgtg tgccggttcc
      781 agcggccgag ataggcgctg gtgccgggct cgaccgggtg cgcgacgccg ttgatcacca
      841 cccggccgcg gccctcgaag aagaacagca gctcgtcctc gccgccgtgc cggtgctcgc
      901 ggacgtggcc gccgggcgcg atcacctgga tgcctgacga gaagccccgg atgtggggcg
      961 aatcgtgctt cgagaccttg acctcggaat agccgttggc cggcaccggc tgccagtagc
     1021 tctggccccg gtcggggccg cacacgacca ccgggcccgg atgcgtctcg gccatgtcgc
     1081 aaaccctccg tgcgtcgcgc ccgggcgtcg ggcacaccga gtcatacacc gcccggcggg
     1141 ccgaatgaag cccgcggccg ggcgagggca cggctcaggt ggggtcgccg gcgaacttgg
     1201 gggtggtcat ccagctctcg ccggcggcgg cgacgcagct caagccgtcg gtgcgggtga
     1261 ccaggaccgt ccaggtgccg gtcgcggcga cgaacagctc gatcaccgtc tcgccttgct
     1321 gttcgaggcc gatcccgacc tgccgctcgc cgtaacgatg gtcgagcttg gcgaccgcct
     1381 cgtcgtgggg cacgcacagc gatacgtccg gctcggcctg cgcggcaccg ccgcctgccg
     1441 ccatcagcag caccgccgcc acagcggcct tcgccttcag gcaatgggtc atctctacct
     1501 ccgctcaggc gcttccgccg tgcctgatga cgtaagctct agcggctccg ccacggcggc
     1561 gctgtgatct ccgtcactgc cgccgttacg tccgcgcaac ggcccggctt gccggcaggc
     1621 cgcgacctgg cgcactgtgg cggctcgagt ctcgatcgga gtcctttcat gtcggtaaag
     1681 ctggtggcgg caatcgtgct cgccgcggtg tcggcggcgg gcgggtagcg gccggtgccc
     1741 gaggcgcgcg ccctgccgct gctgccgacc accgtggtcg gcagctaccc gcagcccgac
     1801 tggctgatcg accgcgacgc gctatcgcac cgctcgccgc cgagggtgcg cgccgcaggg
     1861 ctgtggcggg ttgccgagcc gtggctcgcc gaggcgcagg acgacgccac ccgcctcgcc
     1921 atcgccgaca tggagcgggc cggcatcgac atcatcaccg acggcgagat ccggcgcgaa
     1981 agctactcca accggttcgc caccgcgctc ggcggcatcg accccgcgcg cgccggcacc
     2041 gcacagtcgc gccgcggcag gcccgagccg gtgccgctgg tgagcggccc aatccggcgc
     2101 ctgcgcccgg tcgagaccgc ggacgtggcg ttcctgcgcg ccaacaccgc gcgcaccatc
     2161 aaggcgacgc tgcccggccc gttcaccctg gcgcagcagg cggaggacgc ctattaccgc
     2221 gaccccgagc ggctcgccat ggcctatgcc gaggcggtca acgccgagct gctcgacctg
     2281 ttcgccgccg gcgccgacat cgtccagctc gacgagccct atctccaggc gcggccagag
     2341 gcggcgcgcg cgttcgccgt cgccgccatc gaccgggcgc tcgccggcgc ccccggcacc
     2401 accgcgctgc acctttgcat gggctatgcg gcgctggtcc gcgacaagcc gcccgactat
     2461 gcgttcctgg ccgagcttgc cgacagcgcg gtcgaccagg tctcggtcga ggcggcgcag
     2521 ccgcatgtgg atgtcgcccg cttcgccgac ctcggcgcca agacggtcat cctcggcgtc
     2581 ctcgacctcg ccgacgcggc ggtcgagacg cccgagacgg tggccgcgcg catcgagcgc
     2641 gcgctcgact tcgtgccggc cgagcggctg gtgccggcgc ccgactgcgg catgaagtac
     2701 ctgccgcgcg cggtggcgct gggcaagctc ggcgcgctcg ccgccggcac cgccatggtg
     2761 cgcgcgcgcc tggccgggtg agcggcgctc agaagttgcc gcgcatctga tcgagcgcgc
     2821 tgcccccgcc cggcgccgtg gacggcggcg gcagctgaag ctccgccggc ggcgggcctt
     2881 gcaggatcgt gtcgagcgtc gagggttgcg ccggcggccc gggctgggcc cgcgccggat
     2941 cgatccccgg cacgctcgat gcctcggcga tctccagacg ccggcagccg gactggctgc
     3001 acaccatggt ctcgccggtg gcggtgtcgg tgacgaaggc gatcggatgg ctcgcggcca
     3061 ccgccagcgc gaagcggccg tcggtgccgg ccgcctgcgg cgcggggccg ccgccgagca
     3121 acgccgccgc cgcgatcagc gcggcgccgg caaggatcgc ttggatgttg gtcatggcac
     3181 tctccaaagc ctggtcgctg cccgatcccg ggcgcgctag cggtgcgcgc tcaggaagcg
     3241 gacgccggcc gggccgacgc gctcggcatg cgcgatgccg gcgtcgagcg agaagctgtc
     3301 gcccgcgcgg caggtggtgg tgccggcggc ggtctcgacc gtgatctcgc cgtcgagcac
     3361 caggatcgcg gcgccgaagt cgtggct
//
LOCUS       DASWHU010000080        11279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_29399, whole genome shotgun sequence.
ACCESSION   DASWHU010000080 DASWHU010000000
VERSION     DASWHU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29399"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGB88_02850"
     CDS             complement(<1..276)
                     /locus_tag="VGB88_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9646410.1"
                     /translation="MKIVLKGIHAAANEAVLRRKLALAADIVACDGSEPAGALAEALA
                     EADVAVAMNWRAPLPPVPRLKLLQLPGAGTDGIDPALVPAGAALCNVY"
     gene            complement(377..922)
                     /locus_tag="VGB88_02855"
     CDS             complement(377..922)
                     /locus_tag="VGB88_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646411.1"
                     /translation="MTDSGFRRAVLAAAVLMGGATAAAAEDEQDLTIEFSAPPAVTVN
                     EIEGGEEQFFGTHGGVVDAESPSPLAGAEVTCEFDGYTFPARSFSCGFTTAEAVAGRC
                     LFTTPEGDTAIAEWQCETAATMTSDARCEGRATWIEGTGRFAGIQGTARFHTDLFLHP
                     TEGYARWRGNWGVPRMAALSD"
     gene            complement(1011..2672)
                     /locus_tag="VGB88_02860"
     CDS             complement(1011..2672)
                     /locus_tag="VGB88_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9646412.1"
                     /translation="MTATEGLRHQRDRFVAFAFAGADVLVEVDAGGIVRYAAGALARL
                     DLGGADALVGRPFEDLVEAADRDALRTYLADIGPGVVRRTRWLKLRDASGAPLSVQLS
                     GYRLPDMNDHAFLSIAFADTRRASRGIEPPISTESFTARAAERLSQAAKQEGDFALTV
                     LDLGGFDELVERADAARVDQFLDDVVGRLRSRADGADAVSRFRPDRFGVIHDRQVSLD
                     ADVDWIEADTRDLDPVHRGVSVAQASLALRPEGLSEGDAVRALIYTISQISLGGALDI
                     STLRAGYEAMLDDTLGRLQSFRRLMGATNFGVAFQPIVRLADRKIHHYEALARFAGAA
                     SGVSPFDLISFAEDTGLIAEFDLAMAARVLDTVKEAGPRAPAVAINVSMRSIDSPAFI
                     EKLRALTDGCHRPAGWLMFEITESFKIIDLTRADDALQRLRRAGFAVCLDDFGVGASN
                     LDYLRRLQVDYVKIDGSYVMNLATTPHAKAFLAAIVQLCARLGIKTIAECVESDEVAR
                     LLRDAGVDFAQGFLFGRPEGGLPGALPPARAGADARARAPEPAAP"
     gene            2874..3539
                     /locus_tag="VGB88_02865"
     CDS             2874..3539
                     /locus_tag="VGB88_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9646413.1"
                     /translation="MTAFQRRWHDYYSEKRIVHQWMQVHLLRDLPVRRVLEIGPYLGL
                     VTAMLASAGYRAVTLDVDPGARAGLASHADASVQGDVRDLDSDALAARGFDAVLCCET
                     LEHLPYAQVPAVLARLAAIGAPYLVLSVPYMGPQLSLSLYLNRHLVRARTALKKLAPF
                     KRFPAPAEDGAWEPHKWEVGYRDYPLAGFRALVEAHFRILRTEFTGGCRSVFFLCENR
                     HKR"
     gene            3561..5288
                     /locus_tag="VGB88_02870"
     CDS             3561..5288
                     /locus_tag="VGB88_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9646414.1"
                     /translation="MLILGVHPGYHDASAILCQDYRILAAVQLERLTRRKADGGRFPG
                     EAVDECLAIAGARRADIDVAVLGRGAYPWRYYTHFRGGRWLEGRVREALGRDKMKSME
                     RELVLARRTDSLAIFDAGLFRAEQGLRPDAEVRFYNHHLAHALPTLFHSDWQDALLYT
                     SDGGGDNVQYSHRVFRDGHITTLYGDDSGLVQPLRIDSLGLAYGYATQALGYRINRHE
                     GKLTGLAAYGEPVLYEQFAAHYRVDELGQVHSDFDSYRALRRWVLEIADGRKPADVAA
                     SVQTLLEEVTLRAVKALLARHPVRHLGLSGGVFANVRLNQRLGEECGVDEVFVYPAMS
                     DSGLAAGGVLDYLLARDGLDRWLDRRYRLDSLYFGRDWGAQADAAFAADARLERIAGE
                     PVETAVGLIADGAQVAIFTAGMEYGPRALGARSILAAATDAAINDALNARLGRSEFMP
                     FAPVVAEADAAEVFELAAPLAYAARFMTVTCRVRPAWRERIPAVVHVDGTARPQTLRR
                     DWNPLYYDILNAYKRRTGIPVLINTSFNAHEEPIINTPAECAQALADDRVDYVVTRAA
                     VYRRRAGAA"
     gene            complement(5285..6391)
                     /locus_tag="VGB88_02875"
     CDS             complement(5285..6391)
                     /locus_tag="VGB88_02875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9646415.1"
                     /translation="MHGPNPASENQALILVISSTADPDCVLERLLGDADIGRIRRARS
                     LDAAMPLLGIPEPGPDTVPVELVLIDLGPGAGDAVAACRLIKGSQRHAETPVVVVSDR
                     DDRATLEASFQVGASDYVNRPVDRIELGARVRSVLALKRTLDDRGARAAALERRERDL
                     VEVTRLLEETNERLRHMSTLDALTGISNRRRAMEYLDQEWRRAVRDGTWLSLLMIDVD
                     HFKAYNDALGHQAGDDCLWLVANCLKRCLNRASDLVGRYGGEEFIIALPETPVEGAAS
                     VAEAIRTGIRGLAIDHPASATAPLVTASIGVAGCIPDKLLSVSKLIQYADRALYQAKR
                     QGRNRTVVGRPHGGPSVPVQPGAAALMGTGRATG"
     gene            6659..7834
                     /locus_tag="VGB88_02880"
     CDS             6659..7834
                     /locus_tag="VGB88_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX9646416.1"
                     /translation="MPIVNRIAAFHKDMREWRHDIHQHPELAYQEARTASRVAALLGE
                     FGVDEVVEGIGRTGVVGVIRNGDGPMIGLRADMDALPIVENTGLPYRSRHDGVMHACG
                     HDGHTAMLLGTSRYLAETRNFSGTVVLIFQPAEEGQAGARAMIEDGLFERFPVDAVYG
                     VHNMPGLPAGSIAVSPGAVMAAADGFKITVQGRGGHAAMPHLADDPVIAGCAIVGALQ
                     TLVSRNLDPARTLVISVTQFHGGEAFNVIPDAVELGGTVRYFEAAVGEMARRRMAEVI
                     EGVARAHGVSAAMTYEEGYPPTRNHEAETAFARGVAAEVTGGPAHEQPRIMGSEDFSF
                     FLEAKPGAYAFVGNGDRDGFVCVHNPHYDFNDEILPVGASYFARLVETALPRRRSAA"
     gene            7871..8677
                     /gene="otnI"
                     /locus_tag="VGB88_02885"
     CDS             7871..8677
                     /gene="otnI"
                     /locus_tag="VGB88_02885"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX9646417.1"
                     /translation="MPRFDANLSMLFTELAFLDRFQAAANCGFAGVEVLFPYDWPAGE
                     IAERLLANGLTQVLINVPPGDLEAGERGLGAVPGRESEFRDGLARALDYAEALRCGQV
                     HVMAGMLADDGAAARARARSCFVGNLAWAAAAAAERGVRTLIEPLNTKRDAPGYFLAT
                     VAEARAVIEEVGSNNLLLQCDLYHAQIMTGNLAETIEANLPLIGHFQIAGVPGRHEPD
                     SGEINYPFLFNLIDSLGYRGWVGCEYRPAGATADGLAWAFDYGISGHGGG"
     gene            8749..9786
                     /locus_tag="VGB88_02890"
     CDS             8749..9786
                     /locus_tag="VGB88_02890"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9646418.1"
                     /translation="MAGERDFRSLAPAVQAELRRRAVAMVASGITRVAAAAAVGVSRR
                     YVGKWLKARAAGGDAALEGGQRGRRPGEQLVLKDKAAARIRRLITDKCPDQLKLPFAL
                     WTREAVRALIERECGVRLSLQAVSVYLKRWGFTAQRPMRRASERREPEVARWLAEDYP
                     AIAARAKTEGAEIHWADETGLSNQANYGRSFAPRGRTPVIAHKAERISQSMISSLTNR
                     GKLRFMIYDGAINARIFLAFLKRLVRSCDRKVFLIVDNLRPHRAKLVTAWLEAHTDRI
                     ELLYLPPFAPQHNPDEFVNNDVKQSMARRKPPADKAELRRNLASTMHSLQKRPAKVRA
                     FFQAPTVRYAA"
     gene            10116..10406
                     /locus_tag="VGB88_02895"
     CDS             10116..10406
                     /locus_tag="VGB88_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646419.1"
                     /translation="MHHAKTRQDVGHVLARLAGQAFGLTLVAACAATGYLMFNLTTDS
                     EFAGSIVGFFSAVGRVLGNLFNTNDRVMITVLPALVIAGLLLGGLYVWRKRS"
     gene            complement(10433..>11279)
                     /locus_tag="VGB88_02900"
     CDS             complement(10433..>11279)
                     /locus_tag="VGB88_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9646420.1"
                     /translation="GTAKSPRAAPEIVVEGVHKAFNGNQVLSGIDLEIGCGDIVAIVG
                     DSGCGKTVLMNLMLGLLAPDRGRVRVADHSRPEAPLVDIADLDDTELAGIHTHCGVVF
                     QRNALFGGSVLDNIALWLREVKELEAAAIAPLARKVLAAVDLPTDEAFLDTPSGKLSG
                     GMAKRLAVARALAMDPAVIFFDEPTTGLDPTSAAHVQDLVYAAHYEERREAGARTTVI
                     ITHDKDLLTRLQPRIVMLDEGRVFFDGPFDRFEASTSPTIRPYFDLMPVLHRRAVADR
                     AAGAR"
BASE COUNT         1635 a         3958 c         3992 g         1694 t
ORIGIN      
        1 gtagacgttg cacagcgcgg cgcccgccgg caccagcgcg ggatcgatgc cgtcggtgcc
       61 ggcgccgggc agctggagca gcttcaggcg cggcaccggc ggcagcggcg cgcgccagtt
      121 catcgccacc gccacatcgg cctcggccag cgcctcggcc agcgccccgg ccggctcgct
      181 gccgtcgcag gcgacgatat ccgccgcgag cgcgagcttg cgcctcagca ccgcttcgtt
      241 ggccgcggcg tggatgccct tgagcacgat cttcatggtc gggtacggat cgccgagctc
      301 gtttcaaaaa cggaacggcg gccccgtctc cgggaccgcc gccaggcgga tcggcggctg
      361 ccggcgcggc gccgcctcag tcgctcagcg ccgccatcct cggcacgccc caattgcccc
      421 gccagcgggc atagccctcg gtcgggtgca ggaacaggtc ggtgtggaag cgcgccgtgc
      481 cttggatgcc ggcgaacctg ccggtgccct cgatccaggt cgcccggcct tcgcagcgcg
      541 cgtccgaggt catggtcgcg gcggtctcgc actgccactc ggcgatcgcg gtatcgccct
      601 cgggcgtggt gaacaggcag cgccccgcga ccgcttcggc ggtggtgaag ccgcagctga
      661 agctgcgcgc gggaaaggtg tagccgtcga actcgcaggt gacctcggcg ccggcgagcg
      721 gcgatggcga ctcggcatcg acgacgccgc catgagtgcc gaagaactgc tcctcgccgc
      781 cctcgatctc gttgacggtg accgcgggcg gcgccgagaa ctcgatggtc aggtcctgct
      841 cgtcctcggc cgccgccgcc gtggccccgc ccatgagcac ggccgcggca aggacggcac
      901 gcctgaagcc gctgtcggtc atgttcgctc ccgcgcggtg gttcaacctc tgatcgccat
      961 gatagcgccg aaggcggccg gcggcaaatc gggcggtccg cggtgcgcag tcagggcgcg
     1021 gccggctcgg gggccctggc ccgggcatcg gcgccggccc gcgccggcgg cagcgcgccg
     1081 ggaaggccgc cctcgggcct gccgaacaag aagccctggg cgaagtcgac gccggcgtcc
     1141 ctgagcaggc gcgcaacctc gtcgctttcc acgcattcgg cgatggtctt gatgccgagc
     1201 cgtgcgcaca gctgcacgat cgcggccagg aacgccttgg cgtgcggcgt ggtcgccagg
     1261 ttcatcacgt agctgccgtc gatcttgacg taatccacct gcagccggcg caagtagtcg
     1321 agattgctgg cgccgacccc gaaatcgtcg agacacaccg cgaacccggc ccgtcgcagc
     1381 cgctgcaggg catcgtcggc gcgcgtcagg tcgatgatct tgaaggattc ggtgatctcg
     1441 aacatcagcc acccggccgg gcgatggcag ccgtcggtca gtgcgcgcag cttttcgatg
     1501 aaggccggcg agtcgatcga gcgcatcgac acgttgatcg ccaccgcagg cgcccgcggt
     1561 cccgcctcct tgaccgtgtc gagcacccgg gccgccatcg ccaggtcgaa ctcggcgatc
     1621 aggccggtgt cctcggcgaa gctgatcagg tcgaacgggg aaacgccgga cgccgcgccg
     1681 gcaaatcgcg ccagcgcttc gtaatggtgg atcttgcggt cggcgagcct gacgatcggc
     1741 tggaaggcaa cgccgaaatt ggttgcgccc atcagccggc ggaacgactg caagcgcccg
     1801 agtgtgtcat ccagcatcgc ctcatagccc gcgcgcaggg tgctgatgtc gagcgccccg
     1861 ccaagggaaa tctggctgat ggtatagatc agggcgcgca cggcgtcgcc ttcgctcagc
     1921 ccttcgggcc gcaacgccag gctcgcttgc gcgaccgaca cgccgcggtg cacgggatcg
     1981 aggtcgcgcg tgtcggcctc gatccagtcg acgtcggcgt cgagcgagac ctggcggtcg
     2041 tggatcacgc cgaaacggtc gggacggaac ctgctgaccg cgtcggcgcc gtcggcgcgg
     2101 ctgcgcaggc gcccgaccac gtcgtcgagg aactggtcga cgcgcgccgc gtcggcccgc
     2161 tcgacgagct cgtcgaagcc gccaagatcg agcacggtga gggcgaaatc gccctcctgc
     2221 ttcgccgcct gcgaaagcct ctcggcggcg cgcgcggtga agctttcggt cgagatcggc
     2281 ggctcgatgc cccggctcgc gcggcgggta tcggcgaagg cgatcgacag gaaggcgtgg
     2341 tcgttcatgt ccggcagccg gtagccggac agctggacgc tgagcggcgc accggaggca
     2401 tcgcggagct tgagccagcg cgtccgccgc accacgcccg ggccgatgtc ggcgagatac
     2461 gtccgcaatg cgtcgcggtc cgccgcctcg accaggtcct cgaacggccg tccgaccagg
     2521 gcatcggccc cgcccaggtc gagtcgcgcc agcgcgccgg cggcatagcg cacgatcccg
     2581 ccggcatcga cttcgaccag cacgtcggcg ccggcgaaag cgaaggcgac gaaccggtcg
     2641 cgctgatgcc gcagtccttc ggtggcggtc atgttgttgc gccggcccgc ctacgaagtg
     2701 cgggcgtgcg ggctcgccgg cgcgtcctta ttcttatgca cataagcaac tatcacgttc
     2761 cgtcggccgg gcaacggcag ccgccacgcg cgttgcctgc gggtgcgaaa tttggcaaga
     2821 acggcgggtc ttggccccac agtttgccga tcatgccgga tcagcctcgc gatttgaccg
     2881 ccttccagcg ccgctggcac gactactaca gcgagaagcg catcgttcat cagtggatgc
     2941 aggtgcatct gctgcgcgac ctgccggtgc gccgggtgct cgagatcggg ccctatctcg
     3001 gcctggtcac cgcgatgctg gcctcggccg gctaccgggc ggtgaccctc gacgtggatc
     3061 caggcgcgcg cgccgggctg gcgagccatg ccgatgccag cgtacaaggc gacgtgcgcg
     3121 acctggacag cgacgcgctg gcggcccggg ggttcgatgc ggtgctgtgc tgcgagaccc
     3181 tggagcacct gccctatgcc caggtgccgg cggtgctcgc ccgcctggcc gcgatcggcg
     3241 cgccctacct ggtgctgtcg gtgccctata tgggcccgca gctcagcctg tcgctgtatc
     3301 tcaaccggca cctggtccgc gcccgcacgg cgctcaagaa gctcgcgcca ttcaagcgct
     3361 tcccggcacc ggcggaagac ggggcgtggg agccgcacaa gtgggaggtc ggctatcgcg
     3421 actatccgct ggccggcttc cgggcgctgg tcgaggccca tttccggatc ctgcgcaccg
     3481 agttcaccgg cggctgccgc tcggttttct tcctgtgcga gaaccggcat aagcgctgac
     3541 gtgccgccgc cggcaccgcc atgctgatcc tcggcgtcca tccgggctat cacgacgcct
     3601 ctgcgatcct gtgccaggac taccgcatcc tggccgcggt ccagctcgag cggctgaccc
     3661 ggcgcaaggc cgacggcggc cgctttcccg gcgaggcggt cgacgagtgc ctggccatcg
     3721 ccggggcgag gcgcgcggac atcgacgtgg cggtgctcgg tcgcggcgcg tacccgtggc
     3781 gctactacac ccacttccgc ggcgggcgct ggctcgaggg acgggtgcgc gaggcgctcg
     3841 ggcgcgacaa gatgaagagc atggagcgcg agctggtgct ggcgcggcgc accgactcgc
     3901 tggcgatctt cgatgccggc ctgttccgcg ccgagcaggg cctgcgcccg gacgccgagg
     3961 tcaggttcta caaccaccac ctggcccacg ccctgccgac cctgttccac agcgactggc
     4021 aggacgcgct gctctacacc tcggacggcg gcggcgacaa cgtccagtac agccatcgcg
     4081 tgttccgcga tggccacatt acaaccctgt atggcgacga ttccgggctg gtccagccgc
     4141 tgcgcatcga cagcctgggg ctcgcctacg gctacgccac ccaggcgctc ggctatcgca
     4201 tcaaccggca cgagggcaag ctcaccgggc tcgcggccta tggcgagccg gtgctctacg
     4261 agcagttcgc cgcccactac agggtcgacg agctcggcca ggtgcacagc gacttcgaca
     4321 gctaccgggc gctgcgccgc tgggtgctgg agattgccga cgggcgcaag ccggcggacg
     4381 tggcggcctc ggtccagacg ctgctggagg aggtcaccct gcgcgcggtc aaggcgttgc
     4441 tcgcccgcca cccggtgcgc catctggggc tttcgggcgg ggtgttcgcc aacgtccggc
     4501 tcaaccagcg tctcggcgag gaatgcgggg tcgatgaggt gttcgtctac ccggcgatga
     4561 gcgacagcgg cctcgccgcc ggcggggtgc tcgactacct gctcgcgcgc gacgggctcg
     4621 accgctggct cgaccggcgc tatcggctgg actcgctcta tttcggccgc gactggggcg
     4681 cgcaggcgga cgccgcgttc gcggcggacg cgcgccttga gcggatcgcg ggcgagccgg
     4741 tcgagaccgc ggtcgggctg atcgccgacg gcgcccaggt cgcgatcttc accgccggca
     4801 tggagtatgg gccgcgggcg ctcggcgcgc gcagcatcct ggccgcggcg accgatgccg
     4861 cgatcaacga cgccctcaac gcccgcctcg ggcgcagcga gttcatgccg ttcgcgccgg
     4921 tggtcgccga ggcggatgcg gccgaggtgt tcgagctcgc ggcgccgctc gcctatgcgg
     4981 cgcggttcat gaccgtcacc tgtcgggtgc ggcccgcgtg gcgcgagcgc atcccggcgg
     5041 tggtccacgt tgacggcacc gcccgcccgc agaccctccg gcgcgactgg aacccgctct
     5101 attacgacat cctcaacgcc tacaagcggc ggaccggcat cccggtgctg atcaacacca
     5161 gcttcaatgc ccatgaggag ccgatcatca acacgccggc ggaatgcgcc caggcgctgg
     5221 ccgacgaccg ggtcgactac gtggtcaccc gcgccgcagt ctatcgccgg cgcgcgggcg
     5281 cggcttagcc ggtcgcgcgg ccggtgccca tcaacgccgc ggcgcccggt tgcacaggca
     5341 cgctcgggcc gccatgcggc cgtcccacca ccgtgcggtt gcggccctgg cgcttggcct
     5401 ggtagagcgc gcggtcggcg tattggatca gcttcgagac gctcaacagc ttgtcgggga
     5461 tgcagcccgc gaccccgatg ctggcggtca ccaacggcgc agtcgccgag gcgggatggt
     5521 cgatggcaag cccgcggatg ccggtgcgaa tcgcctccgc gacgctcgcc gcgccctcga
     5581 caggcgtctc gggcagggcg atgatgaact cctcgccgcc atagcgtcca accagatccg
     5641 atgcccggtt gagacagcgc ttgaggcagt tggcgaccag ccacaggcaa tcgtcgccgg
     5701 cctgatggcc aagcgcgtcg ttgtaggcct tgaagtggtc gacatcgatc atcagtagcg
     5761 acagccaggt gccgtcgcgc acggcgcgcc gccattcctg gtcgaggtac tccatcgccc
     5821 ggcgccggtt cgagatgccg gtgagcgcgt cgagcgtcga catgtgcctg agccgctcgt
     5881 tggtctcctc gagtagccgc gtcacctcga cgaggtcgcg ctcgcgccgc tcgagcgccg
     5941 ccgcgcgcgc gccacggtcg tcgagggtgc gcttgagcgc cagcaccgag cgaacccgcg
     6001 cgccgagctc gatgcggtcg accggccggt tgacgtagtc ggaagcgccg acctggaacg
     6061 aggcctcgag cgtggcgcgg tcatcgcggt cgctgaccac gaccaccggc gtctcggcgt
     6121 gccgttggct gcccttgatc aggcggcagg cggcgaccgc gtcgccggcg ccgggcccga
     6181 ggtcgatcag gaccagctcg accggcaccg tgtcgggccc gggctcgggg atgccgagca
     6241 gcggcatcgc ggcgtcgagc gatcttgcgc ggcggatgcg gccgatatcg gcgtcgccaa
     6301 gcaagcgctc gagcacgcag tcaggatcgg ccgtggacga aatgaccagg atcagcgcct
     6361 ggttctcgga tgctggattc ggaccgtgca ctgcggtctc caagctctct gtggtgggtc
     6421 gcacccgggg acgtgcggcc gccggttctg gccgcggaac ccccgaccgg cggctcttcg
     6481 agccagggtg cggttcggtc ggcggggccc aattgaaata gggtgcgccg gcggtcgcgg
     6541 cgagcgatga ttcgatgtcg ttatttgtgt tgagcgctgc cactccgcgt gccgtggcag
     6601 ggcgcccgcg cgcatgctaa cgtcgccgct cgccggcccc ggcgcggagg aggacgcgat
     6661 gccgatcgtc aaccgaatcg ccgcatttca caaggacatg cgcgaatggc ggcacgacat
     6721 ccaccagcat ccggagctcg cctaccagga agcgcggacc gcgtcgaggg tggccgcgct
     6781 gctcggcgag ttcggcgtcg acgaggtggt cgagggcatc gggcggaccg gcgtggtcgg
     6841 cgtgatccgc aacggcgacg gcccgatgat cgggctgcgc gccgacatgg acgcgctgcc
     6901 gatcgtcgag aacacggggc tcccgtaccg ctcgcgtcac gacggcgtga tgcacgcctg
     6961 cggccacgac ggccacaccg ccatgctgct cggcacctcg cgctatctgg ccgagacgcg
     7021 caacttcagc ggcaccgtgg tgctgatctt ccagccggcc gaggagggcc aggccggcgc
     7081 ccgggcgatg atcgaggacg ggctgttcga gcgcttcccg gtcgacgcgg tctacggggt
     7141 gcacaacatg ccggggctgc cggccgggtc gatcgcggtc tcgcccggcg cggtcatggc
     7201 cgcggcggac gggttcaaga tcacggtcca gggccgcggc ggccacgccg ccatgccgca
     7261 cctcgccgac gatccggtga tcgccggctg cgccatcgtc ggcgcgttgc agaccttggt
     7321 ctcgcgcaac ctcgacccgg cccggaccct ggtcatctcg gtgacccagt tccacggcgg
     7381 cgaggcgttc aacgtgattc cggacgcggt cgagctcggc ggcaccgtgc gctatttcga
     7441 ggccgcggtg ggcgagatgg cacgccggcg catggccgag gtgatcgagg gcgtggcgcg
     7501 ggcccatggg gtgagcgccg ccatgaccta cgaggagggc tacccgccga cccgcaacca
     7561 cgaggccgag accgccttcg cccgcggcgt cgccgccgag gtgacgggcg gccccgcgca
     7621 cgagcagccg cggatcatgg gctcggaaga cttctcgttc ttcctcgagg ccaagcccgg
     7681 cgcctatgcc ttcgtcggca acggcgaccg cgacggcttc gtctgcgtcc acaacccgca
     7741 ttacgacttc aacgacgaga tcctgccggt cggcgcgagc tacttcgccc gcctggtcga
     7801 gaccgcgctg ccgcgccggc ggtcggcggc ctaggcggcg gccgggcgcc cgcccgcggg
     7861 gagccgcgcg atgcccaggt tcgacgccaa cctgtcgatg ctgttcaccg agctcgcgtt
     7921 tctcgaccgc ttccaggcgg cggccaattg cggcttcgcc ggggtcgagg tcctgttccc
     7981 ctacgactgg ccggccgggg agatcgccga gcggctgctc gccaacggcc tgacccaggt
     8041 gctgatcaac gtgccgccgg gcgatctgga ggccggcgag cgcgggctcg gggcggttcc
     8101 cgggcgcgag agcgagttcc gcgacgggct ggccagggcg ctcgactacg ccgaggcgct
     8161 gcgctgcggg caggtccacg tcatggccgg gatgctcgcc gacgacggcg cggctgcgcg
     8221 cgcgcgggcg cggtcatgct tcgtcggcaa tctcgcctgg gcggcggcgg cggccgcgga
     8281 gcgcggggtg cgcaccctga tcgagccgct caataccaag cgcgacgcgc cgggctattt
     8341 cctggccacc gtcgccgagg cgcgggcggt gatcgaggag gtcggaagca acaacctgtt
     8401 gctccagtgc gacctctacc acgcccagat catgaccggg aacctggccg agacgatcga
     8461 ggccaacctg ccgctgatcg gccatttcca gatcgccggc gtgcccgggc gccacgagcc
     8521 cgacagcggc gagatcaact accctttcct gttcaacctg atcgactcgc tcggctaccg
     8581 gggctgggtc ggctgcgagt accgtcccgc cggggcgacc gccgacggcc tggcctgggc
     8641 cttcgactac ggcatcagcg gccacggcgg aggctagagc gggatgatat tactaacccg
     8701 ccacgcaaat aggttgcatt tggagtagga ctctgctagt tttgtggcat ggctggagag
     8761 cgggattttc gctcgttggc gcctgcggtg caggcggagc tgcgccggcg cgcggttgcc
     8821 atggtcgctt cggggattac gcgtgttgcg gcggcggcgg cggtgggcgt gagtcggcgc
     8881 tatgtcggca aatggctgaa ggcgcgcgcc gccggcggtg atgcggcgct cgagggtggg
     8941 caacgcgggc gccgaccggg cgagcagctg gtgctgaagg acaaggcggc ggcgcgcatc
     9001 cgccggctga tcaccgacaa atgccccgac cagctgaagc tgccgtttgc gctgtggacg
     9061 cgcgaggcgg tgcgcgcgct gatcgagcgc gagtgcggtg tgcggctgtc cttgcaggcg
     9121 gtcagcgtct atctcaagcg ctgggggttc accgcccaac ggccgatgcg ccgcgccagc
     9181 gagcggcgcg agcccgaggt cgcgcgctgg ctggcggagg attatccggc catcgccgcg
     9241 cgcgccaaga ccgagggcgc ggagatccac tgggccgacg aaaccgggct gtcgaaccag
     9301 gccaattacg gccgcagctt tgccccgcgc gggcgcacgc cggtgatcgc gcacaaagcc
     9361 gagcgcatct cccagagcat gatctcgagc ctgaccaacc gcggcaagct ccgcttcatg
     9421 atctacgacg gcgcgatcaa cgcgcgcatc ttcctcgcct tcctcaagcg tctggtccgc
     9481 agctgcgatc gcaaggtctt cctgatcgtc gacaacctgc gcccgcaccg cgccaagctg
     9541 gtcaccgcct ggctcgaggc gcacaccgac cgcatcgagc tgctctacct gccgccgttc
     9601 gcgcctcagc acaatcccga cgagttcgtc aacaacgacg tcaagcagtc gatggcgcgg
     9661 cgcaagccac ccgccgacaa ggccgagctc cggcgcaacc tcgcctctac catgcatagc
     9721 ctgcagaagc gcccggccaa ggtgcgcgcc ttcttccagg cccccaccgt ccgctatgcc
     9781 gcttgaccag gacatgatac ctatttgaat gtcgagttaa taagtcggat ccctgagcct
     9841 gtttcggtca tcaccccgga ctccgcgaag cggatatccg gggtccatac gcccttgcca
     9901 cgcactcgac cacccgtgca agcggcggga tggaccccgg ctcgcggcgc tgcgcgcctt
     9961 ggccggggtg acgggtgacg gatgaccatg tttcaaatca acaggctcta acctggccga
    10021 tcgcaactgt gacgcctatc actggcatgg accccaaccg gcggttatgt tggtgcctgg
    10081 ctgcgctgca gtgaaggctc gagggaaggc ccccaatgca ccacgcaaag acccgccagg
    10141 acgtaggcca cgtgctcgcc cgcctcgccg ggcaggcgtt cggcctgacg ctcgtcgccg
    10201 cctgcgccgc gaccggctac ctgatgttca acctgaccac cgattcggag ttcgccggtt
    10261 cgatcgtcgg gttcttctcg gctgtcgggc gcgtgttggg caacctgttc aacaccaacg
    10321 accgggtgat gatcacggtg ttgccggcgc tggtcatcgc cggcctcctg ctcggcggcc
    10381 tgtatgtgtg gcgcaagcgg agctgagccc gacccggcgc tgaggccgta gcctatcggg
    10441 cgccggcggc gcggtcggcg accgcgcgcc ggtgcagcac cggcatcagg tcgaaatagg
    10501 gccggatggt cggcgaggtc gaggcctcga accggtcgaa cggcccgtcg aagaacacgc
    10561 ggccctcgtc gagcatgacg atgcgcggct gcaggcgggt cagcaaatcc ttgtcgtggg
    10621 tgatgatcac cgtggtccgc gccccggcct cgcgccgttc ctcgtagtgg gcggcgtaga
    10681 ccaggtcctg gacgtgcgcg gcgctggtcg ggtcgaggcc ggtggtcggc tcgtcgaaga
    10741 agatcaccgc cgggtccatc gccagggcgc gcgcgaccgc gagccgcttg gccatgccgc
    10801 cggacagctt gcccgacggg gtgtccagga acgcctcgtc ggtcggcagg tcgaccgccg
    10861 ccagcacctt gcgcgccagc ggcgcgatcg ccgcggcctc gagctccttc acctcccgca
    10921 gccacagcgc gatgttgtcg agcacgctgc cgccgaacag cgcattgcgc tggaacacga
    10981 ccccacaatg ggtgtggatg ccggcgagct cggtgtcgtc gagatcggcg atgtcgacca
    11041 gcggcgcctc ggggcggctg tggtcggcaa cccggacgcg gccgcggtcg ggcgccagca
    11101 gcccgagcat caggttcatc agcaccgtct tgccgcagcc cgagtcgccg acgatggcga
    11161 cgatgtcgcc gcatccgatc tcgaggtcga tgccggacag cacttggttg ccgttgaacg
    11221 ccttgtgcac cccctcgact acgatctcgg gcgccgccct gggcgatttt gccgtgccg
//
LOCUS       DASWHU010000081         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1506831, whole genome shotgun sequence.
ACCESSION   DASWHU010000081 DASWHU010000000
VERSION     DASWHU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506831"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VGB88_02905"
     CDS             <1..1470
                     /locus_tag="VGB88_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9646421.1"
                     /translation="ADEIMCAEEVADDGAEAEAEVILDQFGNVVPLFAAHHGALNTSS
                     TQTSGYGLGLQGAIDEDLFGHTNQLVVGGSIDLGETEFHSESGIGVLNLNRSVTAGPE
                     PFENTAIFEFETDGVTPEGEVARGEIGPARAVAENTYYGIYVSDTFDVTDNLAVTLAG
                     RFNHADIKITDELDSYFPREVTLAGDHSFSRFNPAAGATYTFPQLRTTAFASYAEANR
                     APSPAELTCAEPTAPCRLPNSFVADPDLEQVVARTFEAGVRGNLPDDWLGAATSLDWA
                     LSFFHSENSNDIIFVSAGPALGTGFFQNVDKTRRRGVEAGLGGNAGRARWFVNYSFIE
                     ATFESGFTVASPNHPRAVDGEIMIEAGDDIPGIPQHNFGLGVDVEVLDGLTVGTSVVA
                     KSGVYLRGDEANLLERTDSYAVVNFRTSYRIAEWLEVFGRIENLFNTDYETFGVLGEA
                     EGTDGAVPIYELPGGVTDPRFLSPGQPFAAFIGMRIRLN"
     gene            1555..2217
                     /locus_tag="VGB88_02910"
     CDS             1555..2217
                     /locus_tag="VGB88_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9646422.1"
                     /translation="MIDQPEWLARSLELLLKGGPVMMVLLAVSVVALAIILVKWGQFA
                     RAGLRNTRFVEPAVDALGRGDREAATTTLRRARHPAARVMEAAIRAAANPRLSADDRD
                     AEIGRVGTAEIRNLESYLRGLEVIANLSPLLGLLGTVLGMIEAFAELEAAGSKVDPAL
                     LAGGIWEALLTTAFGLVIAIPALAAFYLFEGRVDGVRAAMKDGVTRVLGHVLPSRQAA
                     AA"
     gene            2242..2643
                     /locus_tag="VGB88_02915"
     CDS             2242..2643
                     /locus_tag="VGB88_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9646423.1"
                     /translation="MEFATHDRPRPRLSIAPLIDVVFLLLVFFMLASSFIEPAAIDLA
                     MSRPQAAPQAAAEPLVVDVRAGGEVRLNGLALALDQLGPEIAARPGAAGDRPVTVRAE
                     AEVPVALLVAVMDRLRGAGFANLRLATPEAR"
     gene            2657..3070
                     /locus_tag="VGB88_02920"
     CDS             2657..3070
                     /locus_tag="VGB88_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9646424.1"
                     /translation="MRAPEFEGRRRLKGEINLVPLINVVFLLLIFFMLSSTLATPDRF
                     DVDLPESAQARASESEPIVVLVRPDGGVAVNNRTLSIGELEPALSAARAAAPGAGVLL
                     KADARATTADVVSVLRRARAAGIERVALATREAAP"
     gene            3067..3963
                     /locus_tag="VGB88_02925"
     CDS             3067..3963
                     /locus_tag="VGB88_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9646425.1"
                     /translation="MTPIRPIELALALVLAGAVHGAALVGLAVRAPEPAREETEPLVY
                     LQLAALDASAEAPDGVEPQVAATPSPPPPADAPPPVPEALPLPETPELVPPPDLAPVE
                     IAPAPVEVAALEPPADAPPPLPEALPRPEPPVRLPVEIDRPRAKPAPPVPAIEAPPPA
                     QPAVAQTPVRAPMVTPRQQVASLPAVVPPQAVPRNDGRGDALAAYAGELYKLINRTAV
                     TDYPRQAARRGDQGVVPVRLTVGARGELVAVTVVDESAASSPLVRAAVAAVEKAAPFP
                     AFAAAMDSEAETFEVSIVYKLR"
     gene            complement(3977..4720)
                     /locus_tag="VGB88_02930"
     CDS             complement(3977..4720)
                     /locus_tag="VGB88_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646426.1"
                     /translation="MRAHLSVFGAVAFALFALDAAADGLEAAQAAFDHGRFLEAAELA
                     EAEAGADGLALAAEALTVEGYFRSPEDQRQPLLTRAVALAGRAIEADPANARAHLASA
                     RAMGRYAQTIGVLEALNEGYAGRIRAALDQALALDPDLVAALLSLGAWHAEIVATLGI
                     MAGVVYGASEKEALAHYERALALAPDSAAANLEYAIGLLKLDDDEETVARARARALLA
                     HALAVPVEDAYGEIVRERAARRLAQLDGG"
     gene            complement(4724..>4906)
                     /locus_tag="VGB88_02935"
     CDS             complement(4724..>4906)
                     /locus_tag="VGB88_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX9646427.1"
                     /translation="PEHVVKVTVFLTDVADRARINPLRQHYFGDHRPASTLVEISRLV
                     LPELKVEIEAEAVVTD"
BASE COUNT          665 a         1772 c         1787 g          682 t
ORIGIN      
        1 gccgacgaga tcatgtgcgc cgaggaggtc gcggacgacg gcgccgaggc cgaggccgag
       61 gtaatcctcg accagttcgg caacgtggtg cccctgttcg ccgcccacca cggcgcgctg
      121 aacaccagtt cgacccagac cagcggctac ggcctcggcc ttcagggcgc aatcgacgag
      181 gacctgttcg ggcacaccaa ccagctggtc gttggcggca gcatcgacct cggcgagacc
      241 gagttccact cggagtcggg gatcggcgtg cttaacctca accgcagcgt caccgccggg
      301 cccgagccgt tcgagaacac cgccatcttc gagttcgaga ccgacggcgt gacgccggag
      361 ggcgaggtcg cgcgcggcga gatcggcccg gcgcgggcgg tggcggagaa cacctattac
      421 ggcatctacg tctccgacac cttcgacgtg accgacaacc tggcagtgac cctggcgggg
      481 cgcttcaacc acgccgacat caagatcacc gacgagctcg actcgtactt cccgcgcgag
      541 gtcacgctgg ccggcgacca cagcttctcg cgcttcaacc cggcggccgg cgccacctac
      601 accttccccc agctcaggac cacggcgttc gcaagctatg ccgaggccaa ccgcgcgccg
      661 tcgcccgcgg agctgacctg cgccgaaccg accgcgccgt gccggctgcc aaattcattc
      721 gtcgccgacc cggacctcga gcaggtggtc gcccgcacct tcgaggcggg cgtgcgcggc
      781 aacttgcccg acgattggct gggggcggcg acgtcactcg actgggcgct gtcgttcttt
      841 cacagcgaga acagcaacga catcatcttc gtcagtgccg gcccggcgct cggcaccggg
      901 ttcttccaga acgtcgacaa gacccgccgc cgcggcgtcg aggccgggct gggcggcaat
      961 gccgggcgcg cgcgctggtt cgtcaactac agcttcatcg aagcgacctt cgagtcgggc
     1021 ttcaccgtcg cctcgcccaa ccatccgcgc gcggtcgacg gcgagatcat gatcgaggcc
     1081 ggcgacgaca ttcccggcat cccgcagcac aacttcggcc tcggcgtcga cgtcgaggtc
     1141 ctcgacggcc tcaccgtcgg cacctcggtg gtcgccaagt cgggagtcta cctgcgcggc
     1201 gacgaggcca acctgcttga gcggaccgac agctatgcgg tggtcaattt ccgcaccagc
     1261 taccgcatcg ccgagtggct ggaggtgttc gggcggatcg agaacctgtt caataccgac
     1321 tacgagacct tcggcgtcct gggcgaggcc gagggcacgg acggcgcggt gccgatctat
     1381 gagctgccgg gcggggtcac cgacccgcgg ttcctgtcgc cggggcagcc gttcgcggcg
     1441 ttcatcggca tgcgcatccg gttgaactga gcgtgcgccc ttggattctt tgccgcgaat
     1501 cggtacagga ggcagcagcg ggggcacgat cgccggccta gggagggccc gaccatgatc
     1561 gatcagccgg agtggctggc gcggtcgctt gagctgctgc tcaagggcgg gccggtgatg
     1621 atggtgctgc tggcggtctc ggtggtggcg ctggcgatca tcctggtcaa gtggggccag
     1681 ttcgcgcgcg cgggcctgcg caacacccgc ttcgtcgagc cggccgtcga tgcgctcggc
     1741 cgcggcgatc gcgaggcggc gacaacgacc ttgagacgcg cacgccaccc ggcggcgcgg
     1801 gtgatggaag cggcgatccg cgccgcggcg aacccgcggc tgagcgcgga cgaccgcgac
     1861 gccgagatcg gccgggtcgg caccgccgag atccgcaacc tggaaagcta tctgcgcggg
     1921 ctcgaggtga tcgccaacct gagccccctc ctcggcctgc tcggcaccgt gctcggcatg
     1981 atcgaggcgt tcgccgagct cgaggcggcg ggctccaagg tcgacccggc gctgctcgcc
     2041 ggcggcatct gggaggcgct gctgaccacc gccttcggcc tggtcatcgc gatcccggcg
     2101 ctggccgcgt tctacttatt cgagggccgc gtcgatggcg tgcgcgcggc catgaaggac
     2161 ggggtgacgc gggtgctcgg ccacgtcctg cccagccgcc aggcggccgc ggcctgaggt
     2221 ctgcgcagcc ggagcgagtc gatggagttc gccacgcacg atcgcccgag gcctcggctc
     2281 agcatcgcgc cgctgatcga cgtggtgttc ttgctgctgg tgttcttcat gctcgccagc
     2341 tcgttcatcg agccggcggc gatcgacctg gcgatgtcgc gcccgcaggc ggcgccgcaa
     2401 gcggcggcgg agcccctggt ggtcgatgtc cgtgccggcg gcgaggtgcg cctcaacggc
     2461 ctcgcgctgg cgctcgatca gctcgggccg gagatcgccg cgcgccccgg cgccgccggc
     2521 gaccgcccgg tgaccgtgcg cgccgaggcc gaggtgcccg tggcgctgct ggtcgcggtg
     2581 atggaccgat tgcgcggcgc gggcttcgcc aacctccggc tggcgacccc cgaggcgcgc
     2641 tgacggaggc cgggcgatgc gcgcgcccga gttcgagggc aggcggcggc tcaagggcga
     2701 gatcaacctg gtgccgctga tcaacgtcgt gttcctgctg ctgatcttct tcatgctgtc
     2761 gagcaccctc gccacccccg accggttcga cgtcgacctg cccgagtcgg cacaagccag
     2821 ggcgagcgag tccgagccga tcgtggtgct ggtgcgcccg gacggcggcg tcgcggtcaa
     2881 caaccgcacg ctctcgatcg gcgagctgga gccggcgctg agcgcggcgc gcgcggccgc
     2941 gcccggggcc ggggtgctgc tcaaggcgga tgcgcgcgcg accacggccg acgtggtcag
     3001 cgtgctgcgc cgcgcccgcg ccgccggcat cgagcgggtc gcgctcgcca cccgtgaggc
     3061 ggcgccgtga cccccatccg cccgatcgag ctggcgctgg cgctggtgct ggcgggcgcg
     3121 gtgcacggcg cggcgctggt cgggctcgcc gtgcgcgcgc ccgagcctgc tcgcgaggag
     3181 accgagccat tggtctacct ccagctcgcc gcgctcgacg cgagcgcgga ggcgccggat
     3241 ggcgtcgagc cgcaggtcgc ggccacgccc tcgccgccgc cgccggccga cgcgccgcct
     3301 cctgtgcctg aagcgctgcc cctgcccgag acgccggagc tggtgccgcc gccggacctc
     3361 gcgcccgtcg agatcgcgcc ggcgccggtc gaggtcgcgg cgctcgagcc gccggccgac
     3421 gcgccgccgc ccctgccgga ggcgttgccg cggcctgagc cgccggtgcg cctgccggtc
     3481 gagatcgacc gtccgcgcgc caagccggcg ccgcccgtcc cggcaatcga ggcgccgccg
     3541 ccagcgcaac ccgccgtcgc gcaaacgccg gtcagggcac cgatggtaac gccgcgtcaa
     3601 caggtcgcga gcctgcccgc ggtggtcccg ccccaggctg taccgcgcaa cgacggccgc
     3661 ggcgacgcgc tcgccgccta tgcgggcgag ctctacaagc tgatcaaccg gaccgcggtc
     3721 accgactatc cgcgccaggc cgcgcggcgc ggcgaccagg gcgtcgtgcc ggtgcgcctc
     3781 accgtcggcg cgcgcggcga gctggtcgcg gtcacggtgg tcgatgagtc cgccgccagt
     3841 agcccgctgg tgcgcgcggc ggtggccgcg gtcgagaagg ccgccccctt ccccgccttc
     3901 gccgcggcga tggacagcga ggccgagacc ttcgaggtca gcatcgtcta caaactgcgc
     3961 tgagctggcc gccggctcaa ccgccgtcga gctgcgcgag ccgccgcgcg gcgcgttcgc
     4021 gcacgatctc gccgtaagcg tcctcgaccg gaaccgcgag cgcgtgcgcg agcagggcgc
     4081 gggcgcgggc gcgggcgacg gtctcttcgt cgtcgtcgag cttgagcagg ccgatcgcat
     4141 attccaggtt ggccgcggcg ctgtccggcg ccagcgcgag cgcgcgctcg tagtgggcga
     4201 gcgcctcttt ttcgctggcg ccatacacca cgccggccat gatcccgagc gtggcgacga
     4261 tctcggcgtg ccaggcgccg aggctcagaa gcgccgctac caggtcggga tcgagcgcga
     4321 gcgcttgatc gagggcggcg cggatgcgcc cggcgtagcc ttcgttgagt gcttcgagca
     4381 cgccgatggt ctgggcgtag cggcccatcg cgcgcgccga ggcgagatgc gcgcgcgcat
     4441 tggccggatc ggcctcgatc gcccggccgg caagcgcgac cgcgcgggtg agcagcggct
     4501 ggcgctggtc ctcgggcgat cggaagtagc cttcgacggt cagcgcctcg gcggcgagcg
     4561 caagtccgtc ggcgccggcc tcggcctccg cgagctcggc ggcctcgagg aagcgcccgt
     4621 ggtcgaacgc cgcctgcgcc gcctcgagcc cgtctgcggc ggcgtcgagg gcgaagagcg
     4681 cgaacgcaac cgcgccaaag accgaaagat gcgcgcgcat ggctcagtcg gtgaccaccg
     4741 cttcggcctc gatctcgacc ttgagctcgg gcagcaccag ccggctgatc tcgaccaggg
     4801 tcgaggccgg ccggtggtcg ccgaaatagt gctggcgcag cgggttgatg cgcgcgcggt
     4861 cggcgacgtc ggtgaggaac acggtgacct tgacgacgtg ctcggg
//
LOCUS       DASWHU010000082         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2252425, whole genome shotgun sequence.
ACCESSION   DASWHU010000082 DASWHU010000000
VERSION     DASWHU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252425"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VGB88_02940"
     CDS             complement(<1..716)
                     /locus_tag="VGB88_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9646428.1"
                     /translation="MLIGVPREIKDQEYRVGLVPGSVRELTHHGHKVLVESGLGHDIG
                     LDDDDYEAAGAEVVAGPEPVFRRAELLVKVKEPQAGERKWLGEGQVLFTYLHLAADPE
                     QAKDLLESGATAIGYETVTDAAGRLPLLSPMSEVAGRMAIQAGAHCLERAQGGRGVLL
                     GGVAGVQAADVVILGGGVVGTNAARMAMGMEARVTVIDKSLPRLQQLDLQFGAKLNTI
                     FATVEAIERHVLAADLVVGAA"
     gene            complement(742..2190)
                     /locus_tag="VGB88_02945"
     CDS             complement(742..2190)
                     /locus_tag="VGB88_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9646429.1"
                     /translation="MTPSPAAPPRPRAPLDAGAAGRAPRPLRSWRQSLEIYLDRRVVG
                     MLFLGFSAGLPFLLVFSTLTAWLRQAEVSLAAIGFFSWVGITYSIKVFWAPVVDRMPL
                     PLLTRRLGRRRGWMLLAMVGIAAGLLGMAGTDPAQDLTRMALCALLVAFASATQDVAV
                     DAYRIEAVEQSLQGAMAATYQLGYRIALLAAGGGALALADAAGWPLAYRLLASLTLVG
                     MVTVLVIREPAPAVDRAAFGREQRVVRFVERNAHLPRALRGAGAWVIGAVVCPFTDFA
                     ARYRTLAVAVLAFVGLFRISDVLMGAMANPFYLDLGFTLTEIAAIVKVYGTVATIAGA
                     LLGGLAVARYGIFRPLLAGALLLPATNLLFAGLGVIGPEQWMLYVTISADNLSAGFAG
                     TAFIAYLSSLTSAAYTATQYALFSSLMTLPGKLLAGFSGVAVEASGYVAFFVSTAIAG
                     APAVLILLYLMTRARAGAAGSAGARGEAAEDA"
     gene            complement(2234..3211)
                     /locus_tag="VGB88_02950"
     CDS             complement(2234..3211)
                     /locus_tag="VGB88_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX9646430.1"
                     /translation="MPEHRFDVVGIGNAIVDVLSHADDAFLDRHRLAKGAMTLIDNAR
                     ARALYRDMGPAVEMSGGSAANTIAGLASFGAACAFVGKVRDDQLGEVFAHDIRSLGVH
                     FATPPASDGPGTARCLILVTPDAQRTMGTFLGACVELGPEDVTQELIGAARVTYLEGY
                     LWDPPRAKDAFRRAIALAHDAGREVALTLSDAFCVGRWRAEFQALVAGEVDILFANEA
                     ELTSLYQVARFDDALQAVRGKCRLAALTRSEKGSVVLSGEEVHVIDRAPVAHVVDTTG
                     AGDLYAAGFLYGYTRGRSPADCGRLGSLAAAEVISHLGARPEVLLDSLL"
     gene            complement(3310..3717)
                     /locus_tag="VGB88_02955"
     CDS             complement(3310..3717)
                     /locus_tag="VGB88_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9646431.1"
                     /translation="MTRYLLDTDVVSDLVRNPQGLVTQRIREVGEAQVCTSIIVAAEL
                     RYGAAKKGSPRLTAQLEAVLGALEVLPFEAPADAAYGQLRTRLEQAGRPIGGNDLLIA
                     AQAVALNHTLVSGNEREFSRIDGLACENWLRDA"
     gene            complement(3714..3947)
                     /locus_tag="VGB88_02960"
     CDS             complement(3714..3947)
                     /locus_tag="VGB88_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9646432.1"
                     /translation="MAAERHVKLFKNGRNQAVRIPREFELPGDEAIMRKDGDRLIIEP
                     VPSKSLLAALATLTPLDEEFPPIVDVPLEPVEL"
     gene            complement(4025..>4535)
                     /locus_tag="VGB88_02965"
     CDS             complement(4025..>4535)
                     /locus_tag="VGB88_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9646433.1"
                     /translation="HGYEGASLAMLAKASGLQKASLYHHFPGGKQAMAEAVLDEVAAR
                     FERHLFAPLRGDGPPRQRVADMAELLAAYHHDGTEPGLFALLAQGPSRARFAARIAAF
                     YGRWIASLGAALADGGLAAEDSVRRAADMVMAIEGALVLSRALADPGPFRRALRHLVD
                     SLAEPVAAE"
BASE COUNT          674 a         1627 c         1668 g          566 t
ORIGIN      
        1 gccgcgccga ccacgaggtc ggccgcgagc acgtggcgct cgatcgcctc gaccgtggcg
       61 aagatggtat tgagcttggc gccgaactgg aggtcgagct gttgcaggcg cggcagcgac
      121 ttgtcgatca cggtcacccg cgcctccatg cccatcgcca tgcgcgcggc gttggtgccg
      181 accacgccgc cgccgaggat gaccacgtcg gccgcctgga cgcccgcgac cccgcccagg
      241 agcacgcccc gcccgccttg tgcgcgctcg aggcagtggg cgccggcttg gatcgccatc
      301 cggcccgcga cctcgctcat cggcgagagc agcggcaggc ggccggccgc gtcggtcacg
      361 gtctcgtagc cgatcgcggt cgcgcccgac tcgaggaggt ccttagcctg ctcagggtcg
      421 gcggcgaggt gcaggtaggt gaacagcacc tggccctcgc cgagccattt gcgctcgccc
      481 gcctgcggct ccttgacctt gaccagaagc tccgcacgcc ggaacaccgg ctcggggccc
      541 gccaccacct ccgcgcccgc ggcctcgtaa tcgtcgtcgt cgaggccgat gtcgtggccg
      601 aggcccgact cgaccagcac cttatggccg tggtgggtga gctcgcgcac gctccccggc
      661 accagcccga cccggtactc ctggtccttg atctccctcg gcacgccgat cagcatggac
      721 ggtcccccgc tcggtcccgg ttcaggcgtc ttccgccgct tcgccccgcg cgcccgcgga
      781 gccggccgcg ccggcgcgcg cgcgggtcat caggtacaat aagatcagca ccgccggcgc
      841 gcccgcgatc gcggtgctga cgaagaacgc cacatagccg ctcgcctcga ccgcgacgcc
      901 agagaagccg gccagcagct tgccgggcaa ggtcatcagc gagctgaaca gcgcgtactg
      961 ggtcgcggtg taggcggcgc tggtcaggct cgacagatag gcgatgaagg cggtgccggc
     1021 gaagcccgcg ctcaaattgt cggcgctgat ggtgacgtaa agcatccact gctcgggccc
     1081 gatcacgccg aggccggcga acaacaggtt ggtcgccggc agcagcagcg cgccggccaa
     1141 cagcgggcgg aagatgccgt agcgggcgac ggcgaggccg cccaagagcg cgccggcgat
     1201 ggtcgccacc gtgccgtaga ccttcacgat cgccgcgatc tcggtcaggg tgaagccgag
     1261 gtcgagatag aacgggttcg ccatcgcccc catcagcacg tcgctgatcc ggaacagccc
     1321 gacgaaggcg aggaccgcga ccgcgagcgt gcggtagcgc gcggcgaagt cggtgaacgg
     1381 gcagaccacg gcgccgatga cccaggcgcc ggcgccgcgc aaggcgcgcg gcaggtgggc
     1441 gttgcgctcg acgaagcgga ccacgcgctg ctcgcgcccg aacgcggccc ggtcgaccgc
     1501 cggcgcgggc tcgcggatca ccagcacggt gaccatcccg accagggtga ggctggcgag
     1561 cagccggtag gcgagcggcc agccggccgc gtccgcgagc gcgagcgcgc cgccgcccgc
     1621 ggccagcagc gcgatccggt agccgagctg ataggtcgcc gccatcgccc cttgcaggct
     1681 ctgctcaacc gcctcgatgc gataagcgtc gaccgccacg tcctgggtcg ccgaggcgaa
     1741 cgcgaccagg agcgcgcaca gcgccatgcg ggtgaggtct tgcgccgggt cggtcccggc
     1801 catgcccaac aggccggcgg cgatgccgac catggcgagc agcatccagc cgcgccggcg
     1861 cccgagccgg cgggtgagca gcggcagcgg catgcggtcg accactggcg cccagaacac
     1921 cttgatcgag taggtgatgc cgacccagct gaagaagccg atcgcggcca ggctgacctc
     1981 ggcctggcgc agccacgcgg tcagggtcga gaacacgagc aggaacggca gccccgccga
     2041 gaagccaagg aacagcatcc ccaccacccg ccggtcgagg tagatctcga gcgactggcg
     2101 ccacgacctg agcggccgcg gcgcgcggcc cgcggcgccg gcgtcgagcg gcgcccgggg
     2161 gcggggcggt gcggcggggg acggcgtcac ggcggcgcct ttctaccacc gcgccgcgcg
     2221 ccgcgcctac acgctacagc aggctgtcga gcagcacctc ggggcgggcg cccaggtggc
     2281 tgatcacctc ggcggcggca aggctgccga gccggccgca gtcggcgggc gaacgcccgc
     2341 gggtatagcc gtagaggaaa ccggcggcgt agaggtcgcc ggcgccggtg gtgtcgacca
     2401 cgtgggcgac cggggcgcgg tcgatgacgt gcacctcctc gccgctcagg accaccgagc
     2461 ccttctcgct ccgggtcagc gccgccagcc ggcacttgcc gcgcaccgcc tgcagcgcgt
     2521 cgtcgaaccg cgccacctgg tagagcgagg tgagctcggc ctcgttggcg aacaggatgt
     2581 cgacctcgcc ggcgaccagc gcctggaact cggcgcgcca gcgcccgacg cagaacgcgt
     2641 ccgagagggt cagcgcgacc tcgcgcccgg cgtcatgggc gagggcgatg gcgcgccgga
     2701 aggcatcctt ggcgcgcggc gggtcccaca aatagccttc gagataggtc accctggcgg
     2761 cgccgatcag ctcctgggtc acgtcctcgg gcccgagctc gacgcaggcg ccgaggaagg
     2821 tgcccatggt gcgctgggcg tcgggagtga ccaggatcag gcagcgcgcg gtgcccgggc
     2881 cgtcgctcgc cggcggggtc gcgaagtgga cgccgagcga gcggatgtcg tgcgcgaaca
     2941 cctcgccgag ctggtcgtcg cggaccttgc cgacgaacgc gcaggcggcg ccgaacgagg
     3001 ccagcccggc gatggtgttg gccgccgagc cgcccgacat ctccactgcc gggcccatgt
     3061 cgcggtacag cgcgcgcgcc cgggcgttgt cgatcagggt catcgcgccc ttggccagcc
     3121 ggtggcggtc gaggaacgcg tcgtcggcat ggctgagaac gtcgacgatg gcgttgccga
     3181 tgccgacgac gtcgaaccgg tgttcgggca tgggcctcct cgctggctgg aacgggcggg
     3241 cggaagataa cgagccgggc ggcgaccggt tacgccgacg cggtcggccg gtggcgggcg
     3301 cacggccggt caggcatcac gcagccagtt ctcgcaggca aggccgtcga tccgcgagaa
     3361 ctcgcgctca ttgcccgaga ccagggtgtg gttgagggca acggcctgcg ctgcgatcag
     3421 caggtcgttg ccgccgatcg gccggccggc ctgctcgagg cgggtgcgga gctggccgta
     3481 ggcggcatcg gccggcgcct cgaacggcag gacctcgagc gcgccgagca ccgcctcgag
     3541 ctgggccgta agccgtggcg accccttctt ggcggcgccg tagcggagtt ccgcggcaac
     3601 gatgatgctg gtgcagacct gcgcctcgcc gacctcgcga atgcgctgcg tcaccaggcc
     3661 ctgcgggttg cggacgaggt ccgagacgac gtcggtatcg agcaggtagc gcgtcacagc
     3721 tcgacgggct cgagcggcac gtcgacaatc ggcggaaact cctcgtcgag cggcgtcagc
     3781 gtagccagcg ccgccagcag ggatttcgac gggacgggct cgatgatcag ccggtcaccg
     3841 tccttgcgca tgatcgcctc gtcgccggga agctcgaact cgcgtggaat ccgcaccgcc
     3901 tgattgcggc cgttcttgaa cagcttgacg tgccgttcgg cggccattgc agtttccttg
     3961 cgcataggcc acagcatatg ccacccgcgc gccgccgtcc attgcctcga acgggtgggg
     4021 tcggctactc ggcggcgacc ggctcggcga gcgaatcgac caggtggcgg agcgcgcgcc
     4081 ggaacggccc cgggtcggcg agcgcgcgcg acagcaccag cgcgccctcg atcgccatca
     4141 ccatgtcggc ggcccggcgc accgagtcct cggcggcgag cccgccgtcg gcgagcgccg
     4201 cacccaggct ggcgatccag cggccgtaga acgcggcgat gcgggcggcg aagcgcgccc
     4261 ggctcgggcc ctgggcgagc agggcgaaca gcccaggctc ggtgccgtcg tggtggtagg
     4321 cggccagcag ctcggccatg tcggcgacgc gctggcgcgg cggcccgtcc cccctgagtg
     4381 gcgcgaacag atgccgctcg aagcgcgccg cgacctcgtc gagcaccgcc tcggccatcg
     4441 cctgcttgcc gccggggaag tggtggtaca ggctcgcctt ctgcagcccg ctcgccttgg
     4501 ccagcatggc caggctggcg ccctcgtagc cgtgc
//
LOCUS       DASWHU010000083         8064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_31002, whole genome shotgun sequence.
ACCESSION   DASWHU010000083 DASWHU010000000
VERSION     DASWHU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31002"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VGB88_02970"
     CDS             complement(<1..914)
                     /locus_tag="VGB88_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9646434.1"
                     /translation="MIDRSQRDWYKDAVVYQLHTKSFFDSNADGFGDFAGLERKLDYI
                     SYLGVSVIWLLPFYPSPLRDDGYDISDYRNVHRNYGTLRDFRRFLRAAHERGVRVLTE
                     LVINHTSDQHPWFQRARRAKPGSRQRAFYVWSDTNQRYEGTRIIFLDTEISNWAWDSA
                     ANAYYWHRFYSHQPDLNYDNPRVLRAILDAMHFWLGAGVDAMRLDAVPYLCEREGTNN
                     ENLPETHEVLKRLRGALDQRHVNRMFLAEANQWPEDVQHYFGDGDECHMAYHFPLMPR
                     MYMAIAQEDRHPLVEIMQQTPEIPDICQ"
     gene            complement(946..4335)
                     /locus_tag="VGB88_02975"
     CDS             complement(946..4335)
                     /locus_tag="VGB88_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX9646435.1"
                     /translation="MTARHATPRASTPTDTAATGARIYNLFPLLAGPIECWADHLARI
                     AGMGFDWLYLNPFHYPGFSGSLYAVKDPARLHPVVQGDSDQPADVLVGRFVERAAAAG
                     LSVMIDLVAVGVAKDALLVAAHPAWFVRDESGELESPSVGDPSDPRTHTVWGDLARID
                     YGQRRHRRAQLAYWAGYVRRFASLGVRGFRCDAAYQLPAEVWQALIAEARADFDHILF
                     AAETLGCTPKEAEALAPCGFAYLFNSAKWWDFRGDWLLDQYESQRRIAPTIAFPESHD
                     TPRLRAGLAGLSVPEVERWYRLRYAFAAAFSTGVLMPMGYEFGFDQRLDVVRTRPGDW
                     DWQRGQAPFDLSAWIAEVNALKAATPALNVEGGQWRVSAPDADGVALLRADRADRRRA
                     GQVVACVINPRMTGRWSLDAGPLVQAAGGAIGGFDDVTPGGAGGEVGPGAVLSLAPIE
                     LRLLRGRAAPVAAHAPSERDSRRRLDELAARRLAIESVTPQIDGGRHPVKRCIGDVLV
                     VEADILCDGHDQLGARVLYRRGGERRRREAPMARLDNDRWRARAPLAAIGRYSFTIEA
                     WRDLFASWRVEVAKKVDAGQSVVLELTEGQALVETAAGAARGADADALRQALARLADA
                     KGSDGARLGVLMSEELHALMARCGERTNLTRSERELEVVVDRPTARLSAWYEMFPRSQ
                     AGDGKRHGTFDDVIARLPYVRDMGFDVLYLTPIHPIGATNRKGRNNSLVAARRDPGSP
                     YAIGAADGGHDAIHGELGSLDDFRRLVAAAGEHGLEIALDFAIQCSPDHPWLKAHPEW
                     FDWRPDGTIKYAENPPKTYEDIVNAHFYRQALPGIWYALREVVLFWVGQGVRIFRVDN
                     PHTKPLPFWEWLIREVQDRDPQVIFLSEAFTRPKMMMRLAKIGFTQSYTYFTWRTHKQ
                     ELVDYLTELAQGDASECFRPNFFTNTPDINPPVLHGGKRGAFMMRATLAATLSGSWGI
                     YSGFELCEGTPIPGREEYLNSEKYELKAWDWERAGNIRDYIARLNRIRRDNPALHDHT
                     NVRFVNAWNDNVLAYVKMTAAKDNAVLVAVNLDPHAPQGAHFEVPLWEFGLPDAASID
                     VVDLLSGHRFAWHGKVQHVSLDPATAPCALWRLVPPGSASE"
     gene            complement(4414..6564)
                     /locus_tag="VGB88_02980"
     CDS             complement(4414..6564)
                     /locus_tag="VGB88_02980"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9646436.1"
                     /translation="MDRLKRHADIWLALVVLALAVLVRIDEPRLVVDLRNGVFDSYQR
                     WQPRPYLPSPVRIVDIDDDSLARAGQWPWPRTRVAKLVQRLSEMGAAVIVFDILFAEP
                     DRTSPAQILPLWGGDSGLADLARALPDHDQVLADAVAGANVVTGFVLLNQPTESPPPA
                     AKAGFVHSGDDPRPYLAPFAGAVTSLPALEAAAVGNGALNFIPDADGIIRKVPLLVRL
                     GDDLYPTLAAAALGVAQGAGTFVVKASGASDTAGLGAHRGIAAIRVGRIPVPTDANGR
                     MFVHYTGPVPERYLPAWKVLDGSLPDEAVRGMIAIIGTSAAGLMDLRSTPLEPVAPGV
                     MLHAQIMEQVIHQAWLERPDWANGAEVMFMVVLGAAVILLTSRLGAGPTAAVAGAALA
                     GAGALSWYAYGQAHLLFDPVFPALMVAAVYLVSSLVRHMKAERQRRFVERAFSSYVSP
                     NLVQHFIDHPDELRLGGERRDCSFVLTDLAGFTSLVETSDPARLVAVLNEYIDEMTRI
                     AFTHDGTLDRIVGDAVAVIFSAPVIQPDHARRAVACALAMDDFSESFCRAKRADGVPL
                     GHTRIGVNTGPVIIGNVGGSSHSDYRALGDAINTASRLEGVNKYLGTRICIAATTVAQ
                     CDDFIGRPVGTLVLKGKTQGIEAFEPLGRQASSSHRITAYRQAFAKLDRADGEALAEF
                     TGLAERYPDDPLVAFHLARLKSGETGRVVVMAEK"
     gene            complement(6661..7182)
                     /locus_tag="VGB88_02985"
     CDS             complement(6661..7182)
                     /locus_tag="VGB88_02985"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9646437.1"
                     /translation="MNADDLRALQAPLKARYRDHPEAAVITLKAEGKIDAADVACSVE
                     TGRAMVAAGLHPAGGGTGLLACSGDMLLQALVACAGVTLKAVATALGIDLRDARVRAQ
                     GDIDMRGTLGVDRAAPVGFRSIRLAFDIDSDAAPEALDKLLALTERYCVVYQTLTRTV
                     PVTISRYGPSGAA"
     gene            complement(7262..>8064)
                     /locus_tag="VGB88_02990"
     CDS             complement(7262..>8064)
                     /locus_tag="VGB88_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9646438.1"
                     /translation="ARFGGDPAESFTVVAPSLPGYGLSFAPGQTRFAVAEIADCFAEL
                     MTDVLGYRRYAAQGGDWGSFIVSLLAHAHPERLIGIHLNLLPLRRDPKMVADPSPEER
                     AYLDQLAGFLKEETGYQWIQGTRPQTLAFALTDSPAGLAAWIVEKFRTWSDCGGDLDS
                     VFTKDQLLANIALYWFTGAIGSSFWPYYARMHGPWPIPDGATIDVPCGYAEFPREILH
                     PPRSLAARVYSDIRRWSVMARGGHFAAMEQPAALAEEVRAFLRPLRGS"
BASE COUNT         1211 a         2758 c         2950 g         1145 t
ORIGIN      
        1 cactggcaga tgtcgggaat ctccggcgtc tgctgcatga tctcgaccag cgggtggcgg
       61 tcctcctgcg cgatcgccat gtacatgcgc ggcatcagcg ggaagtgata ggccatgtgg
      121 cactcgtcgc cgtcgccgaa atagtgctgg acgtcctccg gccactggtt ggcttcggcc
      181 aggaacatgc ggttgacgtg gcgctggtcg agcgcgccgc gcagacgctt gaggacttca
      241 tgggtctcgg gcaggttctc gttgttggtg ccctcgcgct cgcacaggta gggcaccgcg
      301 tcgagccgca tggcatcgac gcctgcgccc agccagaagt gcatggcgtc gagaatcgcg
      361 cgcagcaccc gcggattgtc gtaattgagg tcgggctggt gcgaatagaa gcggtgccag
      421 taataggcat tcgccgccga gtcccatgcc cagttcgaga tctcggtgtc gaggaagatg
      481 atgcgcgtgc cctcatagcg ctggttggtg tcgctccaga cgtagaacgc gcgctggcgc
      541 gagcccggct tggcgcgccg cgcgcgctgg aaccacgggt gctggtcgga ggtgtggttg
      601 atcaccagct cggtcagcac ccgcacgccg cgctcgtgcg ccgcgcgcag gaaccggcgg
      661 aagtcgcgca aggtgccgta attgcgatgg acgttgcgat agtcggagat gtcgtagccg
      721 tcgtcgcgca gcggcgaggg atagaacggc agcagccaga tcaccgacac gcccagatag
      781 gagatgtagt cgagcttccg ctcgagcccg gcgaagtcgc cgaaaccgtc ggcgttcgag
      841 tcgaagaacg acttggtgtg cagctggtag acgacggcgt ccttgtacca gtcgcgctgg
      901 ctgcgatcga tcatgtcccg ctccggctcg ccgactcgcc gactcttact cgctcgcgga
      961 gccgggcggg accaggcgcc acagggcgca tggcgcggtc gccggatcga gcgagacatg
     1021 ctgcaccttg ccgtgccagg cgaagcgatg cccggacagc aggtcgacga cgtcgatcga
     1081 ggcggcatcg ggcaggccga actcccacaa cggcacctcg aaatgggcgc cctgaggcgc
     1141 gtgcggatcg aggttgacgg cgaccaggac ggcgttgtcc ttcgccgccg tcatcttgac
     1201 ataggccagg acattgtcgt tccaggcgtt gacgaaacgg acgttggtat ggtcgtggag
     1261 cgccgggttg tcgcgccgga tgcgattgag gcgggcgatg tagtcgcgga tgttgcccgc
     1321 acgctcccaa tcccaggcct tgagctcgta cttctcggaa ttgagatact cctcgcgccc
     1381 gggaatcggc gtgccctcgc acagctcgaa gccgctgtag atgccccagc tgccggacaa
     1441 ggtcgccgcc aaggtggcgc gcatcatgaa tgcgccgcgc ttgccgccgt ggaggaccgg
     1501 cggattgatg tcgggcgtgt tggtgaagaa gttgggccgg aagcactcgc tcgcgtcgcc
     1561 ttgggcgagc tcggtgaggt agtcgacgag ctcctgcttg tgggtgcgcc aggtgaaata
     1621 cgtgtaggac tgggtgaagc cgatcttggc caggcgcatc atcatcttcg ggcgggtgaa
     1681 cgcctccgac aggaagatca cctgcggatc gcggtcctgg acctcgcgga tcagccactc
     1741 ccagaacggc agcggcttgg tgtgcgggtt gtcgacccgg aagatgcgca ccccctggcc
     1801 gacccagaac agcaccacct cgcgcagcgc ataccagatc cccggcaacg cctggcggta
     1861 gaaatgggca ttgacgatgt cctcgtacgt cttgggcggg ttctcggcat atttgatggt
     1921 gccgtccggg cgccagtcga accattccgg gtgcgctttc agccacgggt gatcgggcga
     1981 gcactggatg gcgaagtcga gggcgatctc gaggccgtgc tcgcccgccg ccgcgaccag
     2041 gcggcggaag tcgtcgaggc tgccgagctc gccgtggatg gcgtcgtggc cgccgtcggc
     2101 ggcgccgatc gcataggggc tgccgggatc gcgcctcgcc gccaccaggc tgttgttgcg
     2161 gcccttgcgg ttggtggccc cgatcgggtg gatgggcgtg aggtagagca cgtcgaagcc
     2221 catgtcgcgg acatagggaa ggcgcgcgat cacgtcgtcg aaggtgccgt ggcgcttgcc
     2281 gtcgccggcc tgcgagcgcg gaaacatctc gtaccaggcg gagaggcgcg cggtcgggcg
     2341 gtcgaccacg acctcgagct cgcgctcgct ccgggtcagg ttggtgcgct cgccgcagcg
     2401 cgccatcagc gcgtgcagct cctccgacat gagcacgccg agacgcgcgc cgtcgcttcc
     2461 tttggcgtcg gcgaggcgcg ccagcgcctg cctgagcgcg tcggcatcgg ccccgcgcgc
     2521 ggcgcccgcc gccgtctcga ccagggcctg gccctcggtc agctcgagca cgaccgactg
     2581 cccggcatcc accttcttcg ccacctcgac ccgccatgac gcgaacaggt cgcgccacgc
     2641 ttcgatggta aaggaatagc gcccgattgc cgccaggggt gcgcgcgcgc gccagcggtc
     2701 gttgtcgagc ctcgccatcg gcgcctcgcg ccggcgccgc tcgcccccgc ggcggtacag
     2761 cacgcgcgcg ccgagctggt cgtggccgtc gcacaggatg tcggcctcga ccacgagcac
     2821 atcgccgatg cagcgcttga ctgggtggcg gccgccgtcg atctgagggg ttacgctctc
     2881 gatcgccagt cgccgggcgg cgagctcgtc gagccggcgc cggctgtcgc gctcggacgg
     2941 cgcgtgcgcg gccacgggcg cggcgcggcc gcgcaggagg cgcagctcga tcggtgcgag
     3001 cgacagcacg gcgccggggc cgacctcgcc gccggcgccg cccggggtca cgtcgtcgaa
     3061 gccgccgatc gccccgccgg cggcctggac cagcggcccg gcgtccaggc tccagcggcc
     3121 ggtcatgcgc gggttgatca cgcacgccac cacctgcccg gcgcgccgcc ggtcggcgcg
     3181 gtccgcgcgc agcagggcga cgccgtcggc gtcgggcgcg ctcacccgcc actggccgcc
     3241 ctcgacgttg agcgcggggg tcgcggcctt gagcgcgttg acctcggcga tccaggccga
     3301 caggtcgaac ggcgcctggc cgcgctgcca gtcccagtcg cccggccggg tgcgcaccac
     3361 gtcgaggcgc tggtcgaagc cgaactcgta gcccatcggc atcagcacgc cggtcgagaa
     3421 cgcggcggcg aaggcgtagc gcaggcggta ccagcgctcg acctcgggga ccgacagccc
     3481 ggcgagcccg gcgcgcaggc ggggcgtgtc gtggctctcg gggaaggcga tggtcggcgc
     3541 gatccgccgc tggctctcat attggtcgag cagccagtcg ccgcggaagt cccaccactt
     3601 ggccgagttg aacaggtagg cgaagccgca cggcgccagc gcttcagctt ccttcggagt
     3661 gcagccgagc gtctcggcgg cgaacaggat gtggtcgaaa tcggcgcgcg cctctgcgat
     3721 cagcgcctgc cagacctccg ccggcagctg gtaggcggca tcgcagcgga agccccgcac
     3781 gccgagcgac gcgaaccggc gcacataccc cgcccaatag gcaagctgcg cgcggcggtg
     3841 gcggcgctgg ccgtagtcga tgcgcgcaag gtcgccccac accgtatgcg ttcgcgggtc
     3901 ggacgggtcg ccgaccgacg gcgattcgag ctcgcccgac tcgtcgcgca cgaaccatgc
     3961 gggatgggcc gcgaccaaca gcgcgtcctt ggccaccccg accgcgacca ggtcgatcat
     4021 caccgacagc ccggccgcgg cggcgcgctc gacgaagcgc ccgaccagca cgtccgccgg
     4081 ctggtccgaa tcgccctgga ctaccgggtg caggcgcgcc gggtccttga ccgcatacag
     4141 gctgccggag aaccccgggt agtggaacgg gttcaggtac agccagtcga agcccatgcc
     4201 ggcgatgcgc gccaggtggt ccgcccagca ctcgatcggc ccggcgagga gcgggaacag
     4261 gttgtagatg cgcgcgcccg tggccgcggt gtcggttggc gtgctcgctc tcggtgttgc
     4321 gtggcgcgcg gtcatgtccc cggtgacggc aattgggctt cgatgcctaa cccaacatgt
     4381 cgtcgcgccg agcgcaagac cagccgccgc cggtcatttt tcggccatca ccaccacccg
     4441 cccggtctcg ccgctcttga gccgggcgag gtggaaggcg accagcgggt cgtcggggta
     4501 gcgctcggcc aggcccgtga actccgccag cgcctcgccg tcggcgcggt cgagcttggc
     4561 gaacgcctgc cggtaggcgg tgatccggtg cgaggacgac gcctggcggc cgagcggctc
     4621 gaacgcctcg atgccctggg tcttgccctt gagcaccagc gtgccgaccg ggcggccgat
     4681 gaagtcgtcg cactgcgcga ccgtggtggc ggcgatgcag atgcgcgtgc ccagatactt
     4741 gttgacgcct tccaggcgcg aggcggtgtt gatcgcgtcg cccagcgccc ggtagtccga
     4801 gtgcgagctg ccgccgacat tgccgatgat caccgggccg gtgttgaccc cgatgcgggt
     4861 gtggccgagc ggcacgccgt cggcccgctt ggcgcggcag aacgactcgg agaagtcgtc
     4921 catggccagc gcgcaggcga ccgcgcgccg ggcatggtcg ggctggatca ccggcgccga
     4981 aaagatcacc gccaccgcgt cgccgacgat gcggtcgagg gtgccgtcgt gggtgaacgc
     5041 gatccgggtc atctcgtcga tatactcgtt gagcacggcg accagccgcg ccgggtccga
     5101 ggtctcgacc agcgaggtga agccggcgag gtcggtgagc acgaagctgc agtcgcgccg
     5161 ctcgccgccg agcctgagct cgtcggggtg gtcgatgaag tgctgcacca ggttgggcga
     5221 gacgtaggac gagaacgcgc gctcgacgaa ccggcgctgg cgctcggcct tcatgtgcct
     5281 gaccagcgac gagacgagat agaccgccgc caccatcagc gccgggaaga cgggatcgaa
     5341 caacaggtgc gcctggccgt aggcgtacca cgacagcgcg cccgcgcccg ccagcgccgc
     5401 gccggcgacc gccgcggtcg ggccggcgcc gagccgtgac gtgagcagga tcaccgccgc
     5461 gccgagcacg accatgaaca tcacctcggc gccgttcgcc cagtccgggc gctcgagcca
     5521 cgcctggtgg atcacctgct ccatgatctg ggcgtgcagc atcacgcccg gcgccaccgg
     5581 ctcgagcggc gtcgagcgca ggtccatcag gcccgcggcc gaggtgccga tgatcgcgat
     5641 catgccgcgc accgcctcgt ccggcagcga gccgtcgagc actttccacg ccggcagata
     5701 gcgctccggc accgggcccg tgtagtggac gaacatgcgc ccgttggcgt cggtcggcac
     5761 cgggatgcgg ccgacccgga tcgccgcgat tccccggtgc gcgccgagcc cggcggtgtc
     5821 gctggcgccc gaggccttga ccacgaaggt gccggcgcct tgcgccacgc cgagcgccgc
     5881 ggccgcgagc gtcggataga ggtcgtcgcc gagccgcacc aacagcggca ccttgcggat
     5941 gatgccgtcg gcgtcgggga tgaagttgag cgcgccgttg ccgaccgcgg ccgcctcgag
     6001 cgccggcagg ctggtgaccg cgcccgcgaa cggcgccaga tagggacgcg ggtcgtcgcc
     6061 ggaatggacg aagcccgcct tggccgcggg cggcggcgac tcggtcggct ggtttagcag
     6121 cacgaagccg gtgaccacat tggcgccggc gaccgcgtcg gccagcacct ggtcatggtc
     6181 gggaagcgcg cgggcgaggt cggcgaggcc gctgtcgccg ccccacaagg gcaagatctg
     6241 cgccggcgat gtgcggtcgg gctcggcgaa caggatgtcg aacacgatca ccgccgcgcc
     6301 catctcgctc agccgctgca ccagcttggc gacgcgggtg cgcggccacg gccactggcc
     6361 ggcacgggcg aggctgtcgt cgtcgatgtc gacgatgcgc accggcgagg gcaggtaggg
     6421 ccgcggctgc cagcgctggt agctgtcgaa cacgccattc cgcaggtcga ccaccagccg
     6481 cggctcgtcg atgcgcacca gcaccgccaa cgccagcacg accagcgcga gccagatgtc
     6541 ggcgtgccgc ttcagtcggt ccatgccacc ctccggcgac ggcgcacgcg cacgtcgggc
     6601 cgccgatggt agcagcgcac ggcgccttga ccaggggcga gatcgccccg cggccggtgc
     6661 ttacgctgcg ccggagggcc cgtagcggct gatcgtgacc ggcacggtgc gggtgagggt
     6721 ctgatagacg acgcagtagc gctcggtcag cgcgagcagc ttgtcgagcg cctcgggggc
     6781 tgcgtcgctg tcgatgtcga aagcgagccg gatgctgcgg aagccgaccg gcgcggcgcg
     6841 atcgacgccc aaggtgccgc gcatgtcgat gtcgccttgg gcgcgcaccc gggcgtcgcg
     6901 cagatcgatc ccgagcgcgg tggcgactgc cttcaaggtc accccggcgc acgcgaccag
     6961 ggcctggagc agcatgtcgc ccgagcaggc gagcagcccg gtgccgccgc ccgcggggtg
     7021 cagcccggcg gcgaccatcg cccggccggt ctcgaccgag caggcgacgt cggcggcatc
     7081 gatcttgccc tcggccttga gcgtaatcac ggccgcctcg gggtggtcgc ggtagcgggc
     7141 cttgagcggc gcctgcagcg cccgcaggtc atcggcattc atggtgcgcc tcgcagcggt
     7201 tggcgcggcg cactgttcca tggcaagcgg cgcgattcaa cccgcgcccg gtgccgatgc
     7261 gtcacgaccc gcgcaacggc ctgaggaacg cgcgcacctc ctcggccagt gccgcgggct
     7321 gctccatggc ggcgaaatgt ccgccgcgcg ccatcacgct ccagcgtcgg atgtcggagt
     7381 acacgcgcgc cgccagcgag cgcggcgggt gtaggatctc gcgcgggaac tcggcgtagc
     7441 cgcagggcac gtcgatcgtc gcgccgtcgg gaatcggcca cggcccgtgc atgcgcgcat
     7501 agtacggcca gaacgacgag ccgatcgcgc cggtgaacca gtagagcgcg atattggcca
     7561 gcagctggtc cttggtgaac accgagtcga ggtcgccgcc gcagtcggac caggtgcgga
     7621 acttctcgac gatccaggcg gcgaggccgg ccggggaatc ggtgagcgcg aaagcgagcg
     7681 tctgcggccg cgtgccctgg atccactggt agccggtctc ctccttgagg aacccggcga
     7741 gctggtcgag gtaggcgcgc tcctcgggcg aggggtcggc gaccatctta gggtcgcggc
     7801 gcagcggcag caggttgaga tggatgccga tcaggcgctc gggatgcgcg tgggcaagca
     7861 gcgagacgat gaacgagccc cagtcgccgc cctgcgccgc gtagcgccga tagccgagca
     7921 cgtcggtcat cagctcggcg aagcagtcgg cgatctcagc gaccgcgaag cgcgtctggc
     7981 cgggcgcgaa cgacaggccg tagccgggca gcgacggcgc caccacggtg aacgactcgg
     8041 ccggatcgcc gccgaagcgt gccg
//
LOCUS       DASWHU010000084         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2994469, whole genome shotgun sequence.
ACCESSION   DASWHU010000084 DASWHU010000000
VERSION     DASWHU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2994469"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGB88_02995"
     CDS             <1..303
                     /locus_tag="VGB88_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9646439.1"
                     /translation="SALVVAYEPVWAIGTGRTPTTAEVAEVHAFIRDRLAATPGIAAA
                     AVRVLYGGSVKPDNAARLFAVEGVDGGLVGGASLNINDFWTICLSCGKSRRRDARG"
     gene            316..648
                     /gene="secG"
                     /locus_tag="VGB88_03000"
     CDS             316..648
                     /gene="secG"
                     /locus_tag="VGB88_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9646440.1"
                     /translation="MTAVFLVLHVMLAISLVIVVLLQRSDGGALGGLGGGSFGGVMSG
                     RQSANLLTRTTAVLAAGFMGTSLVLAILASGGGAPSSILDTPASQTESAPPAPVEDSG
                     TPSAPLAR"
     gene            729..2357
                     /locus_tag="VGB88_03005"
     CDS             729..2357
                     /locus_tag="VGB88_03005"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9646441.1"
                     /translation="MTRFIFVTGGVVSSLGKGLSAAALGALLQARGYTVRLRKLDPYI
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVPARLSDNVTTGRIYSSVIARE
                     RRGDFLGATVQVIPHVTDEIKSFITADIGGADFVLCEIGGTVGDIEGQPFLEAIRQLG
                     WELGRERTLYVHLTLVPYIRSAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPRG
                     QRQKIALFCNVREHEVIPAIDVDNIYQVPITYHEAGLDTQVLLKFGLDVSRPPDLDRW
                     RDIVERAKGPEGEVSIAVVGKYTGLLDAYKSLSEALTHGGIANNVRVNLDWIDSETFE
                     DGNAVQALEGVHGVLVPGGFGERGAQGKVAAVEFARTRRVPYFGICFGMQLAVIEAAR
                     NLAELPGASSTEFGPCQDPVVGLMSEWTRDGKVERRNTSGELGGTMRLGAYEAVLAEG
                     SRIAGIYGALRISERHRHRYEVNVNYKERLEDAGLVFAGLSPDGTLPETVEIPSHPWF
                     IGVQFHPELKSKPFAPHPLFASFVEAAVHQSRLV"
     gene            2371..3210
                     /gene="kdsA"
                     /locus_tag="VGB88_03010"
     CDS             2371..3210
                     /gene="kdsA"
                     /locus_tag="VGB88_03010"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX9646442.1"
                     /translation="MTAPSHVAIGPVTLGNDLPLAVIAGPCQMESRAHALEMASALKE
                     IAARLGIGLIYKSSYDKANRTSLGGKRGMGLEGALKVFGEIRETLGLPVVTDVHETAH
                     CAIVAPVVDVLQIPAFLCRQTDLLVAAAATGKPVNIKKGQFLAPWDMRHVVAKVTASG
                     NSKVLVTERGASFGYNTLVSDMRSLPILAETGCPVVFDATHSVQQPGGQGGSSGGERR
                     FVPVLARAAVAVGVAGVFIETHPDPDNAPSDGPNMVPLAEFEALIGELQALDRVAKGR
                     AGR"
     gene            3299..>3735
                     /gene="eno"
                     /locus_tag="VGB88_03015"
     CDS             3299..>3735
                     /gene="eno"
                     /locus_tag="VGB88_03015"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909327.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9646443.1"
                     /translation="MSAIIDIVGREILDSRGNPTVEVDVILDDGSHGRAGVPSGASTG
                     THEAVEKRDGGARYRGKGVLQAVAAVNGEIMDALGGVDATDQRHVDRMLIELDGTGNK
                     GRLGANAVLGVSLATAKAAAMAVGMPLYRYLGGANANVLPVPM"
BASE COUNT          568 a         1319 c         1277 g          571 t
ORIGIN      
        1 tcggcgctgg tggtcgccta cgagccggtg tgggcgatcg gcaccggccg caccccgacc
       61 acggccgagg tggccgaggt gcacgcgttc atccgcgacc gcctcgccgc cacgccgggt
      121 atcgccgcgg ccgcggtacg cgtgctatat ggcggctcgg tcaagccgga caacgccgcc
      181 cggctgttcg cggtcgaggg agtcgacggc ggcctggtcg gcggcgccag cttgaacatc
      241 aacgacttct ggaccatctg cctaagctgt ggcaagagcc ggcgacgcga cgcccggggc
      301 tgagggatcg atccgatgac cgcggttttc ttggttttgc acgtgatgct ggcgatctcg
      361 ctggtgatcg tcgtgctgtt gcagcgcagc gacggcggcg cgctcggcgg tctcggcggc
      421 ggcagcttcg gtggcgtcat gagcgggcgc cagtcggcca acctcttgac ccgcaccacg
      481 gccgtgctcg ccgccggctt catgggcacc agcctggtcc tcgcgatcct cgcctcgggt
      541 ggcggcgcgc cgtcctcgat cctggatacg ccggccagcc agaccgaatc agccccgccg
      601 gcgccggtcg aggacagcgg cacgccgagc gcgccgctcg cgcgatagcc gcactccgga
      661 acgccttggc ggccgggtcg cacgggaccc tttgcggcgc ccgttcggga cgagtaggtt
      721 agccgtccat gacccggttc atcttcgtta ccggcggcgt ggtctcatcc ctcggcaagg
      781 gcctgtcggc ggcggcgctg ggcgcgctgc tgcaggcgcg cggctatacc gtgcgcctgc
      841 gcaagctcga cccctacatc aacgtcgacc cgggcaccat gagcccctac cagcacggcg
      901 aggtctacgt caccgacgac ggcgccgaga ccgacctcga cctcggtcat tacgagcgct
      961 tcaccggcgt gcctgcgcgc ctgtccgaca acgtcaccac cgggcgcatc tattcgagcg
     1021 tgatcgcgcg cgagcggcgc ggcgactttc tcggcgcgac cgtgcaggtg atcccccacg
     1081 tcaccgacga gatcaagagc ttcatcacgg ccgatatcgg cggcgccgac ttcgtgctgt
     1141 gcgagatcgg cggcaccgtc ggcgacatcg agggccagcc gttcctcgag gcgatccgcc
     1201 agctcggctg ggagctcggc cgcgagcgga ccttgtacgt gcacctgacc ctggtgcctt
     1261 acatccggtc ggcgggcgag ctcaagacca agccgaccca gcactcggtc aaggagctgc
     1321 gcagcatcgg catccagccc gacatcctgc tgtgccggtg cgaccgcgag atcccgcgcg
     1381 gccagcgcca gaagatcgcg ctgttctgca acgtgcgcga gcacgaggtg atcccggcga
     1441 tcgacgtcga caacatctac caggtgccga tcacctatca cgaggccgga ctggataccc
     1501 aggtgctgct caagttcggc ctcgacgtct cgcgaccgcc cgacctcgac cgctggcgcg
     1561 acatcgtcga gcgcgccaag ggacccgagg gcgaggtgtc gatcgccgtg gtcggcaagt
     1621 acaccggcct gctcgacgcc tacaagtcgc tgtccgaggc gctgacccac ggcggcatcg
     1681 ccaacaacgt gcgggtgaac ctggactgga tcgactcgga gacgttcgag gacggcaacg
     1741 cggtccaggc gctggaaggg gtgcatgggg tcctggtccc cggcggcttc ggcgagcgcg
     1801 gcgcccaggg caaggtcgcg gcggtcgagt tcgcgcgcac ccgccgcgtg ccctatttcg
     1861 gcatctgctt cggcatgcag ctcgcggtga tcgaggccgc gcgcaacctc gccgagctgc
     1921 cgggggcgtc atcgaccgag ttcggcccgt gccaggaccc ggtggtcgga ttgatgagcg
     1981 agtggacgcg cgacggcaag gtcgagcgcc gtaacacgag cggcgagctg ggcggcacca
     2041 tgcggctcgg cgcctacgag gcggtgctcg ccgagggctc gcgcatcgcc ggcatctatg
     2101 gcgcgttgcg gatcagcgag cgccaccgac accgctacga ggtcaacgtc aactacaagg
     2161 agcggctcga ggacgccggc ctggtgttcg ccggcctgtc gcccgacggt acgctgcccg
     2221 agacggtcga gattccgagc cacccctggt tcatcggcgt ccagttccat cccgagctca
     2281 agtccaagcc gttcgccccg caccccctgt tcgcctcgtt cgtcgaagcc gccgtgcacc
     2341 agtcgcggct ggtctgagac cggagtgcgc atgaccgcgc cgagccatgt cgcgatcggg
     2401 ccggtcaccc tcggcaacga cttgccgctg gcggtgatcg ccgggccctg ccagatggaa
     2461 agccgcgcgc acgcactcga aatggcaagc gcgctcaagg aaatagcggc tcgcctcggc
     2521 atcggattga tctacaagtc ttcgtatgac aaggccaacc gcacgtcgct cggcggcaag
     2581 cgcggcatgg ggctcgaagg cgcgctgaag gtcttcggcg agattcgcga gacgctgggc
     2641 cttcccgtcg tcaccgacgt tcacgagacg gcccattgcg ccattgtcgc accggtcgtg
     2701 gacgtactgc agatcccggc gttcctgtgc cgccagaccg atctgctcgt tgccgcggca
     2761 gcgaccggca agcccgtcaa catcaagaag ggccagtttc tcgccccctg ggacatgagg
     2821 cacgtcgtcg ccaaggtgac cgccagcggc aattcgaagg tgctcgtgac cgagcgcggc
     2881 gcgagcttcg gctacaacac gctcgtttcc gacatgcgct cgctgccgat cctcgccgag
     2941 accggctgcc cggtggtgtt cgacgcgacc cattcggtgc agcagccggg cgggcagggc
     3001 ggatcgagcg gcggcgagcg gcgcttcgtt ccggtgctgg cgcgcgccgc cgtcgcggtc
     3061 ggcgtcgccg gcgtcttcat cgagacccat cccgatcccg acaacgcgcc gtcggacggg
     3121 cccaacatgg tgccgctcgc ggaattcgag gcgctgatcg gcgagcttca ggcgctcgac
     3181 cgcgtggcca agggccgggc cggccgatga ggttcgccga cgctttcgaa ccttcatggt
     3241 gacgtgctag agcctgaccg ggctccggcc cgaccattgc ctcgtggaga caccccgcat
     3301 gagcgccatc atcgatatcg tcggccgcga aattctcgac agccgcggca accccaccgt
     3361 cgaggtcgac gtcatcctcg atgacggctc gcatggccgc gcgggcgtcc cctccggcgc
     3421 ctcgaccggg acccacgagg cggtcgagaa gcgcgatggc ggcgcgcgct accgcggcaa
     3481 gggcgttctt caggcggtcg ccgcggtcaa tggcgagatc atggacgcgc tgggcggcgt
     3541 cgatgccacc gaccagcgtc acgtcgaccg gatgctgatc gagctcgacg gcaccggcaa
     3601 caagggccgg ctcggtgcca acgcggtgct cggggtctcg ctcgccaccg ccaaggccgc
     3661 cgcgatggcc gtcggcatgc cgctctaccg ctatctcggc ggcgccaacg ccaacgtcct
     3721 gccggtgccg atgat
//
LOCUS       DASWHU010000085         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2616659, whole genome shotgun sequence.
ACCESSION   DASWHU010000085 DASWHU010000000
VERSION     DASWHU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616659"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="tpiA"
                     /locus_tag="VGB88_03020"
     CDS             complement(<1..627)
                     /gene="tpiA"
                     /locus_tag="VGB88_03020"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9646444.1"
                     /translation="MASMRRPLVAGNWKMNGLRADGVARAAALAAKAAAGAAECDIVL
                     CPPATLITEIAAALAPAEGRLMLSGQDCAPAPGGAFTGDISAPMLADAGCKFVIVGHS
                     ERRAGHGESDAVVRAKAEAAVTAGLVPIVCVGETDTERDAGRALDVVAAQLAGSLPKA
                     AGSALVVAYEPVWAIGTGRTPTTAEVAEVHAFIRDRLAATPGIAAAAVR"
     gene            809..2695
                     /locus_tag="VGB88_03025"
     CDS             809..2695
                     /locus_tag="VGB88_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9646445.1"
                     /translation="MLEALRKRTGSWVVKGFMALLALSFGIWGIGDIFRRPQDPTVAE
                     VGSVKIHASDLQKEYTRTLESLRRMTGGQIDAEQARALGAVGQALDSLVARAAMRQEI
                     DRLSLGVPDDVVRRAITEAPSFRSQTGTFDRMQYLAVLRQNQLSEDEYVGLLRRDLAQ
                     NQLLGSLSAGLVAPRPLVRTLYDFREQRRIADVVTVKSESFSDLSEPDEAALTAYYDA
                     HQDQFTAPEFRRVTFVTIRPADLLDEIVVDDEEVREEYEYRLEEFTTPERRDLDQVVF
                     DDLDTATAAAGRIKAGEDFFAVGSALTGQAEPDMKLGQVTRDALIGEVAAVAFALDAG
                     ALSPPIETPLGWYLVRVNAVSPGGTKSFEDAAEEIRGELKIARAAEATFELGNRFEDE
                     RAGGAKLEDSARPLGLAVETVGPIDRNGYQPDGKTADALPPIQDFLGDAFAAAEGAES
                     ELKEAAGSIFYVLRVDGITPPALRPLAEVRDKVADAWRAEQQRARARASAETLAGRAA
                     EGGLAAAAAAMGLAVETTAAFARDGRDLEISLSNDIITAIFALDPGGVTEAMGLGPDR
                     FVVAQVKELVPAPAPSEEDSATAQIARQLELGMQSDLMAALDDSLRQKYRVRVNNALV
                     ESLF"
     gene            2717..>2876
                     /locus_tag="VGB88_03030"
     CDS             2717..>2876
                     /locus_tag="VGB88_03030"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643682.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX9646446.1"
                     /translation="MQTLPAYDGFEATYQAGAAQVVWTRLVADLETPVSAMLKLADGR
                     PNSFLLESV"
BASE COUNT          432 a         1013 c         1025 g          406 t
ORIGIN      
        1 gcgtaccgcg gccgcggcga tacccggcgt ggcggcgagg cggtcgcgga tgaacgcgtg
       61 cacctcggcc acctcggccg tggtcggggt gcggccggtg ccgatcgccc acaccggctc
      121 gtaggcgacc accagcgccg accccgcagc cttcggcagc gacccggcaa gctgggcggc
      181 gacgacatcc aaggcgcgac cggcgtcgcg ctcagtgtcc gtctcgccga cgcagacgat
      241 cggcaccagg ccggcggtca ccgccgcctc cgccttcgca cgcacgacgg catcgctctc
      301 gccgtggccc gcgcgccgct ccgaatggcc gacgatcacg aacttgcagc cggcatcggc
      361 gagcatcggc gcgctgatgt cgccggtgaa ggcgccgccc ggcgcgggcg cgcagtcctg
      421 gccgctgagc atgagtcggc cctcggcggg ggccaaggcg gcggcaatct cggtgatcag
      481 cgtcgccggc gggcacaaca cgatgtcgca ctccgcggcg ccggccgcgg ccttggcggc
      541 gagcgccgcc gcccgcgcca cgccgtcggc gcgcaacccg ttcattttcc agttgccggc
      601 gaccaacggc cgccgcatcg acgccatggg tgtctccgga cgggatcggg gcgtacgcgc
      661 gcgggcacgg ccgatgtagc acaggtatcg cgcccggcaa aggcgcatga cattgccagt
      721 tgccgcgccg caggccggct cttatcatgc ctgtgcgtcg gccgcggaga tacaattctt
      781 tcaatcactt agggcgaagg ctgcgactat gctcgaagct ttgcgaaagc ggacgggatc
      841 gtgggtcgtc aagggcttca tggcgttgct ggcgctgagc ttcggcatct ggggcatcgg
      901 cgacatattc cgccggccgc aagaccccac ggtcgccgag gtcggctcgg tcaagattca
      961 cgcctccgac ctgcagaagg aatatacccg caccctcgaa tccctgcgcc ggatgaccgg
     1021 cggccagatc gatgccgagc aggcgcgcgc gctcggcgcg gtcggccagg cgctcgacag
     1081 cctggtggca cgcgcggcga tgcgccagga gatcgaccgg ctgtcgctgg gcgtgccgga
     1141 cgacgtggtc cgccgggcga tcaccgaggc gccctcgttc cgcagccaga ccggcacctt
     1201 cgaccgcatg caatacctgg cggtcttgcg ccagaaccag ctgagcgagg acgagtatgt
     1261 cggcctgctg cgccgcgacc tcgcccagaa ccagttgctc ggcagcctgt ccgcggggct
     1321 ggtggcgccg cggccgctgg tgcgcacgct gtacgatttt cgcgagcagc ggcggattgc
     1381 cgacgtggtc acggtcaaga gcgaatcgtt ctcggacttg tccgagcccg atgaggccgc
     1441 gctcaccgcc tattacgacg cccatcagga ccagttcacc gcccccgagt ttcgccgcgt
     1501 caccttcgtc accattcgcc ccgccgatct tctggacgag atcgtggtcg acgacgagga
     1561 ggtgcgcgag gaatacgagt accgtctgga ggagttcacc actcccgagc gccgcgacct
     1621 cgaccaggtg gtgttcgacg acctggatac ggcgacggcc gccgccgggc ggatcaaggc
     1681 gggcgaggac tttttcgcgg tcggcagcgc gctgaccggg caggcagagc ccgacatgaa
     1741 gctcgggcag gttacccgcg acgccctgat cggcgaggtc gccgcggtcg cgttcgcgct
     1801 cgacgccggc gcgctcagcc cgccgatcga gaccccgctc ggctggtacc tggtgcgcgt
     1861 caacgcggta tccccgggcg gcaccaaatc ttttgaggat gccgccgagg agatccgcgg
     1921 cgagctcaag atcgcgcgcg ccgcggaggc gaccttcgag ctcggcaacc ggttcgagga
     1981 cgagcgcgcc ggcggcgcca agctcgagga ttcggcgcgc ccgctcggcc tcgcggtcga
     2041 gaccgtcgga ccaatagacc gcaacgggta tcagcccgac ggcaagacgg cggacgcgct
     2101 gccgccgatc caggactttc tcggcgatgc tttcgccgcc gccgagggcg ccgagtccga
     2161 gctcaaggag gcggccggct cgatcttcta cgtgctgcgt gtcgacggga tcacgccgcc
     2221 ggcgctccgg cccttggccg aggtgcgcga caaggtggca gacgcgtggc gtgccgagca
     2281 gcagcgcgca cgtgcgcgcg cgagcgcgga gacgctggcc gggcgcgcgg ccgagggcgg
     2341 cctggcggcg gcggcggcgg ccatggggct cgcggtcgag accacggcgg cgtttgcccg
     2401 cgacggccgc gacctcgaga tatcgctgtc gaacgacatc atcaccgcga tcttcgcgct
     2461 cgacccgggc ggcgtgaccg aggccatggg gctcggcccc gaccggttcg tggtcgccca
     2521 ggtcaaggag ctggtgccgg cgccggcgcc gagcgaggag gattcggcga ccgcgcagat
     2581 cgcccgtcaa ctcgagctgg gcatgcagtc ggacctgatg gccgcgctcg acgacagctt
     2641 gcgccaaaaa taccgggtgc gggtcaacaa cgcccttgtc gaatcgctgt tctaaagcgc
     2701 gctccaggga cggccgttgc agaccctgcc cgcctatgac ggctttgagg caacctacca
     2761 ggcgggcgcg gcgcaggtcg tctggacgcg cctggtcgcc gacctggaga cgccggtctc
     2821 ggcgatgctc aagctggcgg acgggcggcc caacagcttc ctcctcgaat ccgtcg
//
LOCUS       DASWHU010000086         9934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3355673, whole genome shotgun sequence.
ACCESSION   DASWHU010000086 DASWHU010000000
VERSION     DASWHU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3355673"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGB88_03035"
     CDS             <1..333
                     /locus_tag="VGB88_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9646447.1"
                     /translation="VWERCLSIPGLHGKVPRYPRIAIRYLTLAGKAVGHDAHGSWAAL
                     LQHECDHLDGILYPMRMTDMSTLAFNDSPGPLAEDAAAEAERIDPLFLDLVAHWPGRE
                     RWLAGGAG"
     gene            complement(381..902)
                     /locus_tag="VGB88_03040"
     CDS             complement(381..902)
                     /locus_tag="VGB88_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9646448.1"
                     /translation="MSGARELSSPARLEPHHDLSSFDSGTPVLDDWLRRRALPNQASG
                     ASRTYVVCAEGRVVGYYALAAGAVAHAEATGRARRDMPDPVPVMVIGRLAIDSNYQGC
                     GLGRALLRDAVLRTMQAAEIAGIRAVLVHAISEDARRFYERCGFRASAVDPMTLMLTM
                     QDARHVLIGANRT"
     gene            complement(899..1192)
                     /locus_tag="VGB88_03045"
     CDS             complement(899..1192)
                     /locus_tag="VGB88_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEX9646449.1"
                     /translation="MPNTRTAGRIPRDVSINLRASRTQRSLIDRAAAALGKTRSDFML
                     EASCREAEAVLLDRRYFALDAEAFRDFTAALDAPPSTSPRLRRLLTEKSPWER"
     gene            complement(1279..1767)
                     /locus_tag="VGB88_03050"
     CDS             complement(1279..1767)
                     /locus_tag="VGB88_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX9646450.1"
                     /translation="MDDLASMSGPAAELLKARRQTVAVAESSAGGLISAALLAQPGAS
                     AYFRGGGVIYTAAARAAFLPDPGPSPGWERAATEPYALWLARTLRAHLGADWGLAETG
                     AAGPAGNRYGDPAGHSCIAVAGAIERALTIETGHGRREENMHAFAAAALALLAEALGA
                     RG"
     gene            1833..3023
                     /locus_tag="VGB88_03055"
     CDS             1833..3023
                     /locus_tag="VGB88_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9646451.1"
                     /translation="MRGVVRPLAATMLVQAMVAMAVSAVPVLSPLLAESYGLKASLVG
                     LYASLVFAGAIAFTLLGGVLVRRLGAVRCSQAALAIAAAALAATALGPLAGVVAFAVV
                     TGMGYGLATPAASHILARVTPADRRGVVFSLKQSAVPAGGLLAGLAAPPVAAAFGWQA
                     ALVAVALVVGAAAAVIAPLRAGLDADRNPRAPVSLGAPFAAVARVLGDRSLRALALAT
                     LAFACMQSSLFAIFPAFLVERGGLALIAAGQAFATLQCAGVVARIAMGWASDKLFPAR
                     TLLAGLGFAMAAAGALAAQLDAAWPVAAVFAMAGLIGFVATGWPGIYLAEIVRLVPAD
                     EVGVATGGTVAFSFLGIVAGPALFSAIVAATGSYAPAFYAFGALAAAAGALLWLAARR
                     ARQL"
     gene            3078..3851
                     /locus_tag="VGB88_03060"
     CDS             3078..3851
                     /locus_tag="VGB88_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9646452.1"
                     /translation="MFDLGLAGRVAVVTGGSDGLGRATAERLAAEGCKVAICARRKDH
                     LERAAHDIAEATGAELLAHAADVTKAADIEAFVAATLARFGGVDILVNNAGTSAAASL
                     EEVSDDAWHADIELKVMAAVRLCRRVIPVMRTRGGGSIVNAAIVAAKAPPARALPTSV
                     TRAAGINLTKSLANEYAADNIRVNAICIGLIKSAQWERRAGNRPVEELYAEIAKRVPL
                     GRIGESADYADLVAFLVSERARFITGAAINLDGGMSAVV"
     gene            3859..3990
                     /locus_tag="VGB88_03065"
     CDS             3859..3990
                     /locus_tag="VGB88_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646453.1"
                     /translation="MIAKVAARAQSCPLKGAPVRLDLDLAMLESDPAIPGPCYIPGR"
     gene            4060..4917
                     /locus_tag="VGB88_03070"
     CDS             4060..4917
                     /locus_tag="VGB88_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9646454.1"
                     /translation="MIAAATLCWSMGGILVRLVGADAWTIVFWRSLTLALVLGAYLGL
                     RHGRRTGEMFRAMGPAGALSGLLLAASFVLFILAVTHTLVANALVIMSAAPLVSAVLG
                     RIALGERIRPQTVAAMAAALAGIGVMMWGAGRPGAAPASLLGSLFAFGVALSFGANIV
                     VVRASRHLDMLPAMCLGGLFAALAALPFAAPAEVSARDLGLLALMGSVQLALGLFLFL
                     RGTPHLTAAEVGLLTLLETVLAPIWVWVGVGEEPGPATLAGGAIVLAALVAHSALGIR
                     RSKPPVGMA"
     gene            5006..6343
                     /locus_tag="VGB88_03075"
     CDS             5006..6343
                     /locus_tag="VGB88_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646455.1"
                     /translation="MTEPSTDGGGERPGTETTRARRASLRTLLQTEKTANRVLLAGPV
                     AIAVALLVVVAYSAHVADAGAFLALIGTLFALALASLAAGGFIGFLFGIPRVAPAASE
                     ARTEPAPQAAARPEPGGEAAPARAADAATSRDSGHRVNTNLEEVSDWLTKIIVGVSLV
                     EFKSIAEFVDSLSLDIAGIFQPACTGAVANCFLGVRVFSAAEIILFFLIGIHFGFLWT
                     RLLVQGAIKDADLSLVAKFEQQREELDERVQQDKADADAIHAVGRVLDGSESIAKEEL
                     KRALGNATQRTRAVAFYRARNFRQEHDRTERESIGRVIPIFEALIELDVERTGGRPIY
                     HRNYAQLAYVRKDKTPPDYAGAIEALDAAIRTRPADEAVLYALYEFNRAVCRMQLDKQ
                     FAAGAATDDEEMKQLIVSDLSAACRNEWIRDNVIPSDDEVQKWLKLNKITLRT"
     gene            6395..6865
                     /locus_tag="VGB88_03080"
     CDS             6395..6865
                     /locus_tag="VGB88_03080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9646456.1"
                     /translation="MAEADACPFCSPAEQRFAFAARGGFRAYYNVAPILPGHVLVVPE
                     RHVQSLMGLDDDELGNFFRFARTVTAFVLAAFEGSGFDWSIQEGEDAGQSVDHLHLHV
                     IPRHAGDLPSPGDWYPRLVESRSVRIDDATRPRLTDSERTAIVAQLKERWVDCP"
     gene            7137..8354
                     /locus_tag="VGB88_03085"
     CDS             7137..8354
                     /locus_tag="VGB88_03085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9646457.1"
                     /translation="MVGAGIVGMACALWLKRDGWKVLVLDPRAPGEGCSFGNAGIVAV
                     TQMVPPSTSALLAEVPRMLFDPRWSISIRLRYLPRFAPWLIRFLAASRPARVAEIAQA
                     MAALLGGAMDSHRALLAAAGAEDLLRLQGWLAVYRRATPSLKCDLALRRAHGVRADEL
                     GGAEIRQLVPALARDLDYGVFYPDVGHVRDPFALVQAYARRHLADGGEIARMSVTGFA
                     VRDRRVEAVRTADGELACELAVVAAGAWSRALAAGLGARVPLDTERGYHVTLPEAGVE
                     LPLPVMAGDARFGLTPMIPGLRLAGTAEFAGLDAPPNPARHAAMIERAQRIVPGLGAA
                     RATTWMGFRPSTPDSMPVIGRAPAADNAYLAFGHGHLGLTAGPVTGRAIADLAAGRMP
                     AFDIAPFRAERFH"
     gene            8423..>9934
                     /locus_tag="VGB88_03090"
     CDS             8423..>9934
                     /locus_tag="VGB88_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9646458.1"
                     /translation="MPAQSDRLARPLGGVRVLNLGGAWAGRIAGLLLADQGAEVIELV
                     RPGRAPHRADALVDRGKRVVEIDLKATGGRDRAWALAADADIVLDNMRAGATDRLGLG
                     WRALAAGNPALVYVALPGFAAGDALADAPAWEGVIGARTGVFTDIPPLARVIGGRPVY
                     TAIPMASAYGGVHGALAGTLGLLHARRTGAGQRIEVPLADAVMSAMALLAADIEGQPP
                     RYNIPPIDTTMMEVAAPILRDLADRLAPAHIQRIAAYLKTFRLPLFADYPCGDGRRLF
                     VNATDHVHQTRALLEVLGLYDQLIAEGLVAGNPYADSGAGNNVNDARSMSPAWRERIG
                     AGMAARLKTRPARDWETALRAAGVPATVVQTSAEWLAHPQLHDAGVTADVDDPTLGRT
                     RQPGRFVTIEGAHLASPPLAPRQQLPGEATWRPRAESAPTQRDGAPAGPILAGIRVLD
                     LSNVVAGPAAGRTLAELGAEVIRIDPPAPQAGPRMTMWFGIDVNQGKRAVILDL"
BASE COUNT         1275 a         3577 c         3625 g         1457 t
ORIGIN      
        1 gtgtgggagc gctgcctgtc gatccccggc ctgcacggca aggtaccgcg gtacccccgc
       61 atcgccatcc gctacctgac gctcgccggc aaggcggtcg gccacgacgc ccacggcagc
      121 tgggcggcgc tgctgcagca cgagtgcgac cacctcgacg gcatcctcta cccgatgcgc
      181 atgaccgaca tgtccacgct cgccttcaac gactcgcccg gcccgctggc cgaggacgcc
      241 gccgccgagg cggagcgcat cgatcccctg ttcctcgatc tcgttgcgca ctggcccggg
      301 cgcgagcgct ggctcgccgg cggcgccggc tgagcaagaa ggcccgatcc gagatacacg
      361 cctgcgtgct gtgccggcga tcaggtccgg ttcgcgccga tcagcacatg ccgcgcgtcc
      421 tgcatcgtca gcatcagtgt catgggatcg acggccgagg cccgaaatcc gcaccgttca
      481 taaaaccgtc tggcgtcttc cgagatggca tgcaccagga ccgccctgat acccgcgatc
      541 tcggccgcct gcatcgtgcg cagaaccgcg tcgcgcagca gcgcgcggcc gaggccgcat
      601 ccctggtaat tgctatcgat ggcgaggcgg ccgatcacca tgacgggcac cgggtccggc
      661 atgtcgcgcc gtgcccgccc cgtcgcctcg gcatgcgcga ccgccccggc cgccagtgcg
      721 taatagccga cgacgcgccc ttcagcacag actacgtatg tccgcgacgc gccgctcgct
      781 tggtttggca gggcgcgccg ccgcagccag tcgtcgagca ccggggtgcc cgaatcgaaa
      841 gatgaaagat cgtgatgcgg ttccagcctt gccggtgaac ttagctctcg cgcgccgctc
      901 atcgctccca gggcgatttt tcggtcaaca gccgcctcag cctcggagag gtcgaaggcg
      961 gcgcgtccag cgccgccgtg aagtccctga acgcctcggc gtcgagcgcg aaatatcggc
     1021 ggtcgagcag aaccgcctcg gcctcccggc aggaggcttc gagcatgaag tcggaacgag
     1081 tcttgccgag cgccgctgcc gcccggtcga tcagggacct ttgtgtccgg ctggcgcgca
     1141 aattgatgct cacgtcccgc gggatgcggc ctgccgtgcg cgtgttcggc atgtaagcct
     1201 cctttgtcat cacattcaat atacaagaat gtgtgcacat tgccaatacg atgctcaagg
     1261 cacgtcgcgg cgcgccgttc agccgcgcgc gccgagcgcc tcggcgagca acgccagcgc
     1321 ggcggcggcg aaggcgtgca tattctcctc gcgccggccg tggccggtct cgatggtcag
     1381 cgcgcgctcg atggcgccgg ccaccgcgat gcagctgtga cccgccggat cgccgtagcg
     1441 gttgccggcg ggcccggcgg cgccggtctc ggcgagcccc cagtcggcgc cgagatgggc
     1501 gcggagcgtg cgcgcgagcc acaaggcata cggctcggtc gcggcgcgtt cccagcccgg
     1561 cgaagggccg gggtcgggca gaaaagccgc gcgtgccgcc gcggtgtaga tgacgccgcc
     1621 gccccggaag taggccgagg cgccgggctg ggcgaggagg gccgccgaga tcagcccgcc
     1681 ggccgaggac tcggcgaccg cgaccgtttg ccggcgcgcc ttcaggagct cggccgccgg
     1741 ccccgacatg ctggcgaggt cgtccatcaa ccccgcctac tccccgatcg ccggcgcgtc
     1801 aaacggcgcg tggcgcgcgg cggctgaggg ccatgcgcgg cgtcgtccgt cccttggcgg
     1861 cgaccatgct ggtgcaggca atggtggcga tggcggtgag cgcggtgccg gtgctgtcgc
     1921 cgctgctcgc cgagagctac gggctgaagg cgagcctggt cgggctctac gcgtcgctgg
     1981 tgttcgccgg cgcgatcgcg ttcacgctgc tcggcggcgt gctggtgcgc cggctcggcg
     2041 cggtgcgctg cagccaggcg gcgctcgcca tcgccgcggc ggcgctggcc gcgaccgcgc
     2101 tcggcccgct cgccggcgtg gtcgccttcg ccgtggtcac cggcatgggc tacggcctgg
     2161 cgacgccggc ggcgagccac attctcgcgc gcgtcacccc ggccgaccgg cgcggcgtgg
     2221 tgttctcgct caagcagagc gcggtgccgg cgggcggcct gctcgccggg ctggccgcgc
     2281 cgccggtcgc ggcggcgttc ggctggcagg cggcgctggt cgcggtcgcg ctcgtggtcg
     2341 gggcggcggc ggcggtgatc gcgccgctgc gcgcagggct cgatgccgac cgcaacccgc
     2401 gcgcgccggt ctcgctcggc gcgccgttcg ccgcggtcgc gcgcgtgctc ggcgatcggt
     2461 ccttgcgcgc gttggcgctc gccaccctcg cattcgcttg catgcagtcg tcgctgttcg
     2521 cgatcttccc ggcgttcctg gtcgagcgcg gcggcctcgc gctgatcgcc gccggccagg
     2581 ccttcgccac cttgcagtgc gcgggcgtgg tcgcgcgcat tgccatgggc tgggcgagcg
     2641 acaagctgtt tcccgcgcgc accctgctcg ccgggctcgg cttcgcgatg gcggcggcgg
     2701 gcgcgctcgc cgcccagctc gacgccgcct ggccggtggc ggcggtgttc gcgatggcgg
     2761 gcctgatcgg cttcgtcgcc accggctggc cgggaatcta cctggccgag atcgtgcgcc
     2821 tggtaccggc ggacgaggtc ggcgtcgcca ccggcggcac ggtcgcgttc agctttctcg
     2881 gcatagtcgc cgggccggcg ctgttcagcg ccatcgtcgc cgcgacgggc agctacgcgc
     2941 ccgcgttcta cgccttcggc gccctcgccg ccgccgccgg tgccctgttg tggctcgccg
     3001 cccggcgagc gcgccagctt tgagcgaccg cgcccgaccg gtataaggtt ggtgcctgcg
     3061 acgggcgagg aggggacatg ttcgatctcg gactggcagg gcgggtggcg gtcgtgaccg
     3121 gcggcagcga cgggctgggg cgggcgaccg ccgaacggct cgcggcggag gggtgcaagg
     3181 tcgcgatctg cgcgcggcgc aaggaccatc tggagcgggc ggcgcacgac atcgccgagg
     3241 cgaccggcgc cgagctgctc gcgcacgccg ccgacgtgac caaggccgcc gacatcgagg
     3301 cgttcgtggc ggcgacgctc gcgcgcttcg gcggcgtcga catcctggtc aacaacgccg
     3361 gaacctcggc cgcggcatcg ctggaagagg tcagcgacga cgcctggcac gccgacatcg
     3421 agctcaaggt aatggcggcg gtgcgcttgt gccgacgcgt gatcccggtg atgcggacgc
     3481 gcggcggcgg cagcatcgtc aatgccgcga tcgtcgccgc caaggcgccg ccggcgcgcg
     3541 cgctgccgac ctcggtgacg cgcgccgccg gcatcaactt gaccaagtcg ttggccaacg
     3601 agtacgccgc cgacaacatc cgggtgaacg cgatctgcat cggcctgatc aagagcgcgc
     3661 agtgggagag gcgcgccggc aaccgcccgg tcgaggagct gtacgccgag atcgccaagc
     3721 gcgtgccgtt gggccgcatc ggcgagtcgg cggactacgc ggacctggtc gcgttcctgg
     3781 tgtccgagcg cgcgcgcttc atcaccggcg ccgcgatcaa cctcgacggc ggcatgtcgg
     3841 cggtggtctg acatgccgat gatcgccaag gtcgcggcgc gcgcgcagtc gtgcccgctc
     3901 aaaggcgcgc cggtgcgcct cgacctcgat ctcgccatgc tcgagtcgga cccggcgatc
     3961 cccggcccct gctacatccc gggccgctag gccgcacgcc gccgtccgga ggatcgcgcc
     4021 atagcgacgt cgatcacgcc gcgactgcgc ggcgcgctgc tgatcgcggc cgcgaccttg
     4081 tgctggagca tgggcgggat tctcgtgcgc ctggtcggtg ccgacgcctg gaccatcgta
     4141 ttctggcgct cgctcacgct cgcgctcgtg ctcggcgcct atctcggctt gcgccacggg
     4201 cggcggacgg gcgagatgtt tcgcgccatg ggcccggccg gtgccttgtc cgggctgctg
     4261 ctcgcggcgt cgttcgtgtt gttcatcctc gcggtcacgc acaccctggt cgccaacgcg
     4321 ctggtgatca tgagcgcggc gcccctggtc tcggccgtgc tcgggcggat cgcgctcggc
     4381 gagcgcatca ggccgcagac ggtggcggcg atggcggcgg cgctggcggg catcggcgtc
     4441 atgatgtggg gcgcgggccg gccgggcgcg gcgccggcgt cgttgctcgg cagcctgttc
     4501 gcgttcggcg tcgcgctcag cttcggcgcc aacatcgtgg tggtgcgcgc ctcgcgccac
     4561 ctcgacatgc tgccggcgat gtgcctgggc gggctgttcg ccgcgctcgc cgcgctgccg
     4621 ttcgcggcac cggcggaggt gtctgcgcgc gacctcggcc tgctcgcgct gatgggcagc
     4681 gtgcagctcg cgctcggcct gttcctgttc ctgcgcggca ccccgcacct gacggccgca
     4741 gaggtcggcc tgctcacctt gctcgagacg gtgctggcgc cgatctgggt gtgggtggga
     4801 gtgggcgagg agccggggcc tgcgacgctc gccggcggcg cgatcgtgct cgccgcgctc
     4861 gtcgcgcatt ccgcgcttgg cattcgccgc agcaagcctc cggtcggcat ggcttgagcg
     4921 cggcgcgccg gaacggccgc gacaggtcgg cgcatgtggt ctacgatgcc gcgcttcggc
     4981 acgggcgcgg gaggaggggt gagacatgac cgagccctcg acggacggcg gcggcgaaag
     5041 gcccggcacg gagacgactc gcgcgcgcag ggcgagcctg cggacgctgc tgcaaaccga
     5101 gaagaccgcc aaccgggtgc ttctcgccgg accggtcgcg atcgcggtcg ctctcctggt
     5161 cgtcgtcgcc tattcggcgc acgtcgccga cgccggcgcg ttcctcgcct tgatcggcac
     5221 gctgttcgcg cttgcgctcg cgtcgctggc cgcgggcgga ttcatcggct tcctgttcgg
     5281 tatcccgagg gtcgcgccgg cagcgagcga ggcgcggacg gaaccggcgc cgcaagccgc
     5341 cgccaggccg gagcccggcg gcgaagcggc gcccgcccga gcggcggacg cggctacctc
     5401 cagggacagc ggccaccgcg tcaacaccaa cctggaagag gtgtccgact ggctgaccaa
     5461 gatcatcgtc ggcgtgtcgc tggtcgagtt caagtcgatc gccgagttcg tcgacagctt
     5521 gtctttggac atcgccggca tctttcagcc cgcttgcacc ggagcggtcg cgaactgctt
     5581 cctcggcgtc agggtgttct cggcggcgga gatcatcctg ttctttctca tcggcatcca
     5641 tttcggcttc ctgtggaccc ggctgctggt ccagggcgcg atcaaggacg ccgacctgtc
     5701 gctggtggcg aagttcgagc agcagcgcga ggagctggac gagcgcgtgc agcaggacaa
     5761 ggccgacgcc gatgcgatcc atgccgtggg ccgggtgctc gacgggtcgg aaagcatcgc
     5821 caaggaggag ctcaagcgcg cgctggggaa cgccacccag cgcacgcgcg cggtggcatt
     5881 ctatcgggca cgcaacttcc gccaggagca cgatcgcacc gagcgggaaa gcatcgggcg
     5941 cgtgataccg atcttcgagg cgctgataga gctcgacgtc gagcgaaccg gcgggcggcc
     6001 gatctatcat cgcaactacg cgcagctcgc ctatgtgcgc aaggacaaga cgccgcccga
     6061 ctatgcgggc gcgatcgagg cgctggacgc ggcgatcagg acccggccgg cggacgaggc
     6121 ggtgctctac gccctgtacg agttcaaccg ggcggtgtgc cgcatgcaac tcgacaagca
     6181 gtttgcggcc ggcgcggcaa ccgatgacga ggagatgaag cagctgatcg tgagcgacct
     6241 ttcggccgca tgccggaacg aatggattcg agacaacgtg attccgtccg acgacgaggt
     6301 ccagaagtgg ctcaagctga acaagataac gctccgcacg taagcctcgc gcgtggtgcc
     6361 ggcggcgccc tgcggcgagc cgcagccgac gcgcatggcc gaggctgacg cttgcccgtt
     6421 ctgctcgccc gcggagcaga gattcgcgtt cgccgcgcgc ggcggctttc gtgcctacta
     6481 caacgtcgcg ccgatcctgc ccgggcacgt cctggtggtc cccgaacgac acgtccagag
     6541 cctgatgggg ctcgatgacg atgagctcgg caacttcttc cgcttcgcgc gcacggtgac
     6601 cgcattcgtg ctcgcggcgt tcgaggggag cgggttcgac tggtcgatcc aggagggcga
     6661 ggacgcgggg cagagcgtcg accacctcca tcttcacgtc atcccgcggc atgccggcga
     6721 cctgccgtcc ccgggcgact ggtatccgcg cctggtcgaa tctcggtcgg tgcggatcga
     6781 cgatgccacg cgccctcggc taaccgatag cgagcgaacg gcaatcgtgg cccaactcaa
     6841 agagcgttgg gtggactgcc catgatgaag ccggccagcc acgcggaggc gcggaggacg
     6901 cggagggtgc ggagaagagt gaaaccgcgg aggacacgga ggacacagag agcacagagt
     6961 gtgtcattct ctatgatctc tgcgcgctct gcggttgatc cttttcgctc acctccgcgt
     7021 cctccgcgcc tccgcgtgat ccgaataacg gcgcgcggca cattgtccgt gaagcggtga
     7081 gtgaaacaag cgcaggggct gccggcgaca gccgccgtgg gcgcgggcgg gcggtggtgg
     7141 tcggcgccgg catcgtcggc atggcgtgtg cgctgtggct caagcgtgac ggctggaagg
     7201 tcctggtctt ggacccgcgc gcgccgggcg aagggtgctc gttcggcaat gccggcatcg
     7261 tcgcggtcac ccagatggtg ccgccgtcga cctccgcact cctcgccgag gtgccgcgca
     7321 tgctgttcga cccgcgctgg tcgatctcga tccgcctgcg ctacctgccg cgcttcgccc
     7381 cgtggctgat ccgcttcctc gccgcgagcc ggccggcacg cgtcgccgag atcgcgcaag
     7441 cgatggccgc gctcctcggc ggtgcgatgg acagccaccg cgcgctgctc gccgccgccg
     7501 gggccgagga cctgctccgg ctgcagggct ggctcgccgt gtaccggcgc gcgacgccgt
     7561 cgctcaagtg cgacctcgcg ctcaggcgcg cccacggcgt gcgcgcggac gagctcggcg
     7621 gggcggagat tcgccagctc gtgccggcgc tcgcgcgcga cctcgactac ggcgtcttct
     7681 accccgacgt cggccacgtg cgcgacccgt tcgccctcgt ccaggcctat gcccgccggc
     7741 acctcgccga cggcggggag attgcgcgca tgtccgtgac cgggttcgcg gtccgcgacc
     7801 gcagggtcga ggcggtgcgg acggcggacg gcgagctggc gtgcgagctg gcggtggtcg
     7861 cggccggcgc ctggtcgcgc gcgcttgccg ccgggctcgg cgcgcgcgtg ccgctcgata
     7921 ccgagcgcgg ctaccacgtc accctgccgg aggccggcgt cgagctgccg ctgccggtga
     7981 tggccggcga cgcgcgcttc gggctgaccc cgatgatccc cggcctgcgc ctcgccggca
     8041 ccgccgagtt cgcaggtctc gatgcgccgc ccaaccccgc ccgccacgcg gcgatgatcg
     8101 agcgcgcgca aagaatcgtg ccgggcctcg gcgccgcgcg ggcgacgacc tggatgggct
     8161 ttcgcccctc gacgcccgac tcgatgccgg tgatcgggcg cgcgccggcg gccgacaacg
     8221 cctatctcgc tttcggccac ggccatctgg gcctgaccgc gggccctgtg acggggcgcg
     8281 cgatcgccga cctcgccgcc gggcgcatgc ccgccttcga catcgccccg ttccgcgccg
     8341 agcgtttcca ctgagccggt ttgggcttgt ggggccggcc cgcgcggggc acccttcggc
     8401 gtcacgcgag gggagtgcga cgatgccggc tcaatcggac cgccttgccc gcccgctcgg
     8461 cggcgtgcgc gtgctcaacc tgggcggcgc ctgggcgggg cggatcgcgg gcctgttgct
     8521 cgccgaccag ggggcggagg tgatcgagct cgtccggccg gggcgcgcgc cgcaccgtgc
     8581 cgatgccctg gtcgaccgcg gcaagcgggt ggtcgagatc gacctcaagg ccacgggcgg
     8641 gcgcgaccgg gcgtgggcgc tcgccgccga cgccgacatc gtgctcgaca acatgcgcgc
     8701 cggcgccacc gaccgactcg gcctcggctg gcgcgcgctc gccgcgggca atccggccct
     8761 ggtttatgtc gcgctgccgg gcttcgccgc gggcgatgcg ctcgccgacg cgcccgcctg
     8821 ggagggcgtg atcggcgccc gcaccggcgt tttcaccgac atcccgccgc tcgcgcgagt
     8881 gatcggcggc cgcccggtct ataccgcgat cccgatggcc tcggcctatg gcggcgtcca
     8941 cggcgcgctc gccgggacac tcgggctgct gcacgcacgg cgcaccggcg ccggccagcg
     9001 catcgaggtg ccgcttgcgg acgcggtgat gtcggcgatg gcgctgctcg ccgccgacat
     9061 cgagggccag ccgccgcgct acaacatccc gccgatcgac accaccatga tggaggtcgc
     9121 ggcgccgatc ctgcgcgatc tcgccgaccg gctcgcgccc gcgcacatcc agcgcatcgc
     9181 cgcctacctc aagaccttcc gcctgccatt gttcgccgac tatccgtgcg gcgacggccg
     9241 gcgcctgttc gtcaacgcca ccgaccacgt ccaccagacg cgggcgttgc tcgaggtgct
     9301 cggcctctac gaccagctga tcgccgaagg gctggtcgcc ggcaacccct atgccgacag
     9361 cggcgccggc aacaatgtca acgacgcgcg ctcgatgtcg ccggcctggc gcgagcgcat
     9421 cggcgccggc atggcggcgc gcctcaagac ccgcccggcg cgcgactggg agacggcgct
     9481 gcgtgccgcc ggcgtgccgg cgacggtggt ccagaccagc gccgagtggc tggcgcaccc
     9541 gcagctccac gacgccgggg tgaccgccga cgtcgacgac ccgacgctcg ggcgcacccg
     9601 ccagccgggg cggttcgtga ccatcgaggg cgcgcacctc gcctcgccgc cgctcgcgcc
     9661 gcgccaacag ttgccgggcg aagcgacctg gcggccccgc gcggagagcg caccgacgca
     9721 gagggacggc gcgccggcgg ggccgatcct cgccggcatt cgcgtgctcg acctgtcgaa
     9781 cgtggtcgcc gggccggcgg cggggcgcac gctcgccgag ctcggcgccg aggtgatccg
     9841 catcgacccg ccggcgcccc aggccggccc gcgcatgacc atgtggttcg gcatcgacgt
     9901 caaccagggc aagcgcgcgg tgatcctgga cctg
//
LOCUS       DASWHU010000087         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1886806, whole genome shotgun sequence.
ACCESSION   DASWHU010000087 DASWHU010000000
VERSION     DASWHU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886806"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VGB88_03095"
     CDS             complement(<1..780)
                     /locus_tag="VGB88_03095"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9646459.1"
                     /translation="MAAPDVTDIAGIWPMLYAFFDTGGGLDRAAIAAEVDALAASGVH
                     GIAALGLASEVNKLDVNERRAFLDCVAEALGGRLPLAVTVAEPSVAGQRAFAAAAEAA
                     GARWLILQPPPAPGASEAELVRFFGAVADTTPLPVAIQNAPQYIGVGLGNQALATLGR
                     RHPNLRLLKAEGPPVYLKRLIAETEGRFRVFNGRAGLELTESLRLGCAGLIPGAESAD
                     VQAAIYDLARAGQWDRAEAMFRDLLPLIAFLMDSIPHLVCYG"
     gene            869..1516
                     /locus_tag="VGB88_03100"
     CDS             869..1516
                     /locus_tag="VGB88_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646460.1"
                     /translation="MGLASPTLTLFEDRLAPGAVLGPAPAAGHRVIYVAEGAATVDGA
                     AAAEDEARYGAADASIAAGRSGAVLWRWQVADGDVPAGAGSTGKLAVALARHGIGRGR
                     WLLRCDSVWFPPGGCAYLHTHRGPGIRCLRTGSIRIDTAGTSTRYRPGEAWFEPGPEP
                     VFAQGDAEHETRFIRVMVLPPALAGQPSITYVRSEDRDRPKSQRYRGYCDIPVDL"
     gene            complement(1559..>1676)
                     /locus_tag="VGB88_03105"
     CDS             complement(1559..>1676)
                     /locus_tag="VGB88_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017998776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9646461.1"
                     /translation="TKYRPCPLLVKYVEAGWIGRKAGRGFYDYSGDKPVPTR"
BASE COUNT          216 a          620 c          621 g          219 t
ORIGIN      
        1 gccgtagcac accaggtgcg gaatcgagtc catcaggaac gcgatcagcg gcagcaggtc
       61 gcggaacatc gcctcggcgc ggtcccattg gccggcgcgg gcaaggtcgt agatcgccgc
      121 ctgcacgtcg gcgctctcgg cgcccgggat caggccggcg cagccgagcc tgagcgactc
      181 ggtgagctcg agcccggcgc ggccgttgaa cacgcggaac cgcccctcgg tctcggcgat
      241 caggcgcttg agatacactg gcggcccctc ggccttgagc aggcgcaggt tcgggtggcg
      301 gcggccaagc gtcgcgagcg cctggttgcc gagcccgacg ccgatatatt gcggcgcgtt
      361 ctggatcgcg accggcaacg gcgtggtgtc ggcgacggcg ccgaagaagc gcaccagctc
      421 cgcctcgctc gcgcccggcg ccggcggcgg ctgcaggatc agccagcgcg cgcccgcggc
      481 ctcggcggcg gcggcgaacg cgcgctggcc ggcgacgctc ggctcggcga cggtcaccgc
      541 cagcggcagg cgcccgccga gcgcctcggc gacgcagtcg aggaaggcgc ggcgctcgtt
      601 cacatcgagc ttgttgacct cgctggcgag cccgagggcg gcgatgccgt gaaccccgct
      661 cgccgcgagc gcatcgacct cggccgcgat cgcggcgcgg tcgaggccgc cgccggtgtc
      721 gaagaacgca tacagcatcg gccagatgcc ggcgatgtcg gtcacgtccg gcgccgccat
      781 gcgcgctccc ccgttcgtcg tggcgcacga ttatgcccgt gcgcgcgcga ttgcgcgact
      841 cgcgccccgc gcctagagtt gcccgcccat gggccttgcg tcccccacgc tcaccctgtt
      901 cgaggaccgg ctggcgccgg gcgcggtcct tggcccggcg ccggccgccg gccatcgcgt
      961 gatctacgtc gccgagggcg cggccaccgt cgacggcgcg gccgcggccg aggacgaggc
     1021 gcggtacggg gcggcggacg cctcgatcgc cgccggacgc tcgggcgcgg tgctgtggcg
     1081 ctggcaggtc gcggacgggg acgtgccggc aggggccggc tcgaccggga agctcgcggt
     1141 cgcgctcgcg cgccacggca tcggccgcgg gcgctggctg ctgcgctgcg acagcgtctg
     1201 gttcccgccc ggcggctgcg cctatctcca cacccaccgg ggacccggca tccggtgcct
     1261 gcgcacgggc tcgatccgca tcgacaccgc cggcacgtcg acccggtacc ggccgggcga
     1321 ggcgtggttc gagccgggcc cggagccggt gttcgcgcaa ggcgacgccg agcacgagac
     1381 ccggttcatc cgggtgatgg tgctgccgcc cgctctcgcc ggccagccgt cgatcaccta
     1441 tgtgcgcagc gaggaccgcg accggcccaa gtcgcagcgc taccgcggct actgcgacat
     1501 tccggtcgat ctataaggca gcaatgattg ccgcggcaac ggtctccttg cgaccgtgtc
     1561 agcgcgtcgg caccggcttg tcgcccgagt agtcgtagaa gccgcgcccg gccttgcgcc
     1621 cgatccagcc ggcctcgaca tacttaacca gcagcgggca gggacggtac ttggtg
//
LOCUS       DASWHU010000088         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_779383, whole genome shotgun sequence.
ACCESSION   DASWHU010000088 DASWHU010000000
VERSION     DASWHU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779383"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VGB88_03110"
     CDS             complement(<1..714)
                     /locus_tag="VGB88_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9646462.1"
                     /translation="MTEPQTIARLLAAGADDSTAIAAPGRAPLTNKDLRRLAGEVRAA
                     LNAHGIGRGDRVAIVLANGPEMAAAFLTVACAATTAPLNPAYRDEELDFYLSDLGARA
                     LMVEAGADTPARAVAARLGVPICELTADARAAGLFTLAGPAQPRAIEAGAAEADDVAL
                     VLHTSGTTSRPKIVPLSHANLCASARHIGATLKLGAGDLCLNVMPLFHIHGLVAGVLS
                     SLAAGAAVACTPGFNALRFY"
     gene            839..2188
                     /locus_tag="VGB88_03115"
     CDS             839..2188
                     /locus_tag="VGB88_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9646463.1"
                     /translation="MSVTDIETAETLPGVPNNLQPLWKPFTANRQFKAAPRLLVAAEG
                     MYYRSIDGRQILDGAAGMWCVNAGHGRREIAEAVKRQVAVMDYAPAFQMGHPLEFDLA
                     ARLAALLPGDLDHLFFTNSGSESVDTALKIALAYHQARGEPTRTRLIGRERGYHGVGF
                     GGMSVGGIAANRRQFRNLIAEVDHLPHTHAPEHNAFSRGQPEWGAHLADQLEALVQLH
                     GAGTIAAVIVEPVAGSTGVLVPPMGYLHRLRELCDRHGILLIFDEVITGFGRLGASFA
                     SEYFDVLPDIATMAKGLTNATVPMGAVAVRKGIYDAFMTGPEHVIELFHGYTYSGHPL
                     ACAAGLATLSLYGEEDLFERAAEMAPHFEDAVHGLRGHRHVIDVRNLGLMAGIELEPR
                     PGAPGTRALDVFRDCFARGVLMRITGDTLALSPPLIIERAEVDRLVETIAASLAGTA"
     gene            2185..3381
                     /locus_tag="VGB88_03120"
     CDS             2185..3381
                     /locus_tag="VGB88_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646464.1"
                     /translation="MNIGAPNYAEEQLGATLRNIAGNPLGWQAVHLHLSGLAQRHRRE
                     GTVRIALKGLQELVHAHEGRIFVLFNFDIVLLVRGALVSEVQALVDATRALFTDDRLS
                     REARLFSTWYDLSITLGRLEREVRRLALDKLRLREGRRSESTRFFNLEPLDPGRLYKL
                     QAALATLDISGYVRHQPVCALLPHAMPRRVFEEIYVHIADLQKPLMPNVNLAGNLWLF
                     QHLTRSLDQRVLAMLTRQPEDLKSRPISLNMNIDTILSQSFLAFDDSLKQGLQRRIVI
                     EVQPVDVFADVKAFQLGREFVRRKGYRLCLDGLKPETLPLCGRASLGVDLIKLHWDDS
                     MAGSGSEAAAGDLGRAIREADSKRVILTRCDDQEAVEFGFALGINLFQGRHIDEMLRP
                     GSHSRN"
     gene            complement(3599..>3853)
                     /locus_tag="VGB88_03125"
     CDS             complement(3599..>3853)
                     /locus_tag="VGB88_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646465.1"
                     /translation="QMRQQVQGAFGDLFPVRQMQELSKQNMEMFRQALAMFTPLAGTG
                     GAATAAKPERGEAGHDREISELRDQLARMQKQLDSLAEGD"
BASE COUNT          556 a         1366 c         1367 g          564 t
ORIGIN      
        1 gtagaagcgg agcgcgttga accccggcgt gcacgccacc gcagccccgg cggcaagcga
       61 ggacagcacg cctgcgacca gcccgtggat gtggaacagc ggcatgacgt tgaggcacag
      121 gtcgcccgcc ccgagcttga gcgtcgcgcc aatgtgccgc gccgaggcgc acaaattggc
      181 gtggctcaag ggcacgatct tgggccgcga ggtggtgccc gaggtatgga ggaccagcgc
      241 cacgtcgtcg gcctcggccg cgccggcctc gatcgcgcgc ggttgcgccg ggccggcgag
      301 ggtgaacagg ccggcggcgc gcgcatccgc ggtgagctcg cagatgggaa cgccgaggcg
      361 cgcggccacc gcccgcgccg gggtgtcggc gccggcctcg accatcagcg cgcgcgcgcc
      421 gagatcggac aggtagaaat cgagctcctc gtcgcggtag gcgggattga gcggcgcggt
      481 ggtcgcggcg caggcgacgg tcaggaacgc cgccgccatc tcggggccgt tggcgagcac
      541 gatcgcgacc cggtcgccgc ggccgatgcc gtgggcgttg agcgcggcac ggacctcgcc
      601 ggcgaggcgg cgcaagtcct tgttggtgag gggagcgcgc ccgggcgcgg cgatggcggt
      661 ggagtcgtcg gcgccggcgg cgagcagccg ggcaatggtc tggggctcgg tcatgggcgg
      721 gttttagcgc ggaacgggcg cgccgccaat cgccggcgcc ttcgccacgg ggcacgcgat
      781 cgtttaccat ccggaaagcg ttgcgggcgg acaatcacag cagcgggaga gaccagcgat
      841 gagcgtcacc gacatcgaga ccgccgagac cctgccgggg gtgcccaaca acctccagcc
      901 gctgtggaag ccgttcaccg ccaaccgcca gttcaaggcg gcgccgcggc tgctggtcgc
      961 ggccgagggc atgtactacc gctcgatcga cggccgccag atcctcgacg gcgccgccgg
     1021 catgtggtgc gtgaatgccg gccacggccg gcgcgagatc gccgaggccg tcaagcgcca
     1081 ggtcgcggtg atggactacg cgccggcgtt ccagatgggc catccgctcg agttcgacct
     1141 cgcggcgcgc cttgccgcgc tgctgcccgg cgacctcgac cacctgttct tcaccaactc
     1201 gggctccgaa tcggtcgaca ccgcgctcaa gatcgcgctc gcctaccacc aggcgcgcgg
     1261 cgagccgacc cgcacccgcc tgattgggcg cgagcggggc tatcacggcg tcggcttcgg
     1321 cggcatgtcg gtgggcggca tcgccgccaa ccggcgccag ttccgcaacc tgatcgccga
     1381 ggtcgaccac ctgccgcaca cccacgcgcc cgagcacaac gccttcagcc gcggccagcc
     1441 ggagtggggc gcgcacctcg ccgaccagct cgaggcgctg gtccagctcc acggcgccgg
     1501 caccatcgcg gcggtgatcg tcgagccggt ggccggctcg accggcgtgc tggtgccgcc
     1561 gatgggctat ctgcaccgcc tgcgcgagct ctgcgaccgg cacggcatcc tgctgatctt
     1621 cgacgaggtg atcaccggct tcggccggct tggcgcgagt tttgctagcg agtatttcga
     1681 cgtcctgccg gacatcgcga ccatggccaa ggggctgacc aacgccaccg tgccaatggg
     1741 cgcggtggcg gtgcgcaagg ggatctacga cgcgttcatg accgggcctg agcacgtgat
     1801 cgagctgttc cacggctaca cctattccgg ccatccgctc gcctgcgccg ccgggctcgc
     1861 gaccctgtcg ctgtacggcg aggaggacct gttcgagcgc gccgccgaga tggcgcccca
     1921 cttcgaggac gcggtgcacg gactgcgcgg ccacaggcac gtgatcgacg tgcgcaacct
     1981 cggcctgatg gcgggaatcg agctcgagcc gcgccccggc gcgccgggca cgcgcgcgct
     2041 cgacgtgttc cgcgactgct tcgcgcgcgg ggtgctgatg cgcatcaccg gcgatacgct
     2101 ggccctgtcg ccgccgctga tcatcgagcg cgccgaggtc gaccggctgg tcgagacgat
     2161 cgcggcctcg ctcgccggga cggcatgaac atcggcgcgc ccaactacgc cgaggagcag
     2221 ctcggcgcca ccctcaggaa catcgccggc aacccgctcg gctggcaggc ggtgcacctg
     2281 cacctgtccg ggctcgccca gcgccatcgc cgcgagggca ccgtgcgcat cgctctcaag
     2341 ggcttgcagg agctggtgca cgcgcacgag gggcgcatct tcgtgctgtt caatttcgac
     2401 atcgtgctgc tggtcagggg cgcgctggtc agcgaggtgc aggcgctggt cgacgccacc
     2461 cgcgccctgt tcacggacga tcggctgagc cgcgaggcgc gcctgttctc gacctggtac
     2521 gacctgtcga tcaccctcgg ccggctcgag cgcgaggtcc gccggctggc gctcgacaag
     2581 ctgcgcctgc gcgaggggcg caggtccgag agcacgcgct tcttcaacct cgagccgctc
     2641 gatccgggcc ggctgtacaa gctccaggcc gcgcttgcga ccctcgacat ctcgggctat
     2701 gtgcgccacc agccggtgtg cgcgctgctg ccccacgcca tgcccaggcg cgtgttcgag
     2761 gagatctacg tgcacatcgc ggacctgcag aagccgctga tgccgaacgt caacctggcc
     2821 ggcaacctct ggctgttcca gcacctgacg cgctcgctcg accagcgggt gctggcgatg
     2881 ctgacccgcc agccggagga cctcaagtcg cggccgatca gcctcaacat gaacatcgac
     2941 accatcctgt cgcagagctt cctcgccttt gacgacagcc tcaagcaggg cctccagcgc
     3001 cggatcgtga tcgaggtgca gccggtcgac gtgttcgccg acgtgaaagc gttccagctc
     3061 ggccgcgagt tcgtgcgccg caagggctac cggctgtgcc tcgacgggct caagcccgag
     3121 accttgccgc tgtgcggtcg cgcgagcctc ggcgttgacc tgatcaagct gcattgggac
     3181 gactcgatgg ccggcagcgg cagcgaggcg gcggcgggcg atctcggccg ggcgatccgc
     3241 gaggccgatt cgaagcgcgt gatcctcacc cgctgcgacg accaggaggc ggtcgagttc
     3301 ggcttcgcgc tcggcatcaa cctgtttcag ggccgccaca tcgacgagat gctgcgcccg
     3361 ggcagccaca gccgcaactg accggcgggc gcgcgctcga agtttgtcga tttgccgcct
     3421 cacccttcga gacgcgccct gcgggcgctc ctcagggtga ggtaaccgta tgatcttaaa
     3481 gaattcctcg tcctgaggag gtcgcgaccc gtcctccgca gcagcggcgg cgccgctgcc
     3541 gctgcggagg gtggacgcgg ccgtctcgaa ggacggcggc cgtgtcgttc gcgcgcgctc
     3601 agtcgccttc ggcgagcgag tcgagctgtt tctgcatgcg cgcgagctgg tcccggagct
     3661 cgctgatctc gcggtcgtgg ccggcctccc cgcgctccgg cttcgccgcc gtcgccgcgc
     3721 cgccggtgcc ggcgagcggc gtgaacatgg ccagggcctg gcggaacatc tccatgttct
     3781 gcttgctcag ctcctgcatc tggcggaccg ggaacaggtc gccgaacgcg ccctggacct
     3841 gctggcgcat ctg
//
LOCUS       DASWHU010000089         9093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_35073, whole genome shotgun sequence.
ACCESSION   DASWHU010000089 DASWHU010000000
VERSION     DASWHU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35073"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VGB88_03130"
     CDS             complement(<1..1202)
                     /locus_tag="VGB88_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9646466.1"
                     /translation="MATQPGVKRKLAAILSADVVGYSRLMQADEAATVAALKTCREVI
                     AGLVARHGGRVVNAPGDALLADFASAVEAVAAAVEIQQAVEGRNVELAPERRMRLRIG
                     VNLGDVIEEADGTVYGDGVNIAARLEALAEAGGICVSGAVHDAVEGKLDCGFDFLGER
                     PVKNIAKPVRVYAVGAAGRPRADAAAAGHQDIRFCSARDGTSIAYAVAGAGPPLVKTA
                     NWLNHLEYDWTSPIWRHLLAELAHDHMLVRYDARGNGLSDWTVEDISFEAFVSDLETV
                     VDACRLDRFPLLGISQGCAVSIAYAVRHPERVSKLVLYGGFARGRRKRGSQADIDQAE
                     AMITLIRHSWGQDNPAIRQLFATLFLPDGTPAQVDWFNDLQRITTSPENAARIRTATN
                     DIDVTALLA"
     gene            complement(1240..2700)
                     /locus_tag="VGB88_03135"
     CDS             complement(1240..2700)
                     /locus_tag="VGB88_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9646467.1"
                     /translation="MYDQAVAAHKRAQGQAQAVRMLIGGAWRDGADSYQDRDPYRGEV
                     VATVPVSTAADVDAAVEAAVAAKDRAAAVPGYQRAALLRRVGTLLAERTGDIARAMAR
                     ETGKAIRDCAAEVERSAETIALSAEEAVRITGEQVPLEASAMGAGRIAFTLRVPVGVV
                     AAITPFNAPFNLACHKVAPAIAAGNAVVLKAPPQAPLSVHKLAELFVDAGAAAGLLNV
                     LYGEAAGPALVAHPKVDMISFTGSSATGARIRAACGLKRVALELGGDGPTIVHADADI
                     DAAATMCARNAMRLAGQSCISVQKVFVHRAVADSFTHRLVAAVDALKVGDPLEEATDV
                     GTCIDEDAARRVESWVAEALAGGARRLAGGHRVGAQYAPCVLADVEASMKVVCEEVFG
                     PVVSVIPYDDIDQAFARINASRYGLQCGLFSASLAVALSAIRRIRTGGVIINGTSTWR
                     TDQLAYGGVKDSGLGREGPHYAIREMTEERLVVFNG"
     gene            2797..3639
                     /locus_tag="VGB88_03140"
     CDS             2797..3639
                     /locus_tag="VGB88_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX9646468.1"
                     /translation="MVESVRYFGINTYSYIYSHAAGPCLEALAARGHTHFELMMYPGH
                     AWPQDMDARARQRLRRAIDAGGLTLCTLNMPNIDVNIAAAAPAMRELSRPHLKRVISL
                     AGDLGAAGVVVGPGKPNPLFPGPVDQMTGWFYQALDVLVPLAERAGTRVLVENMPFAF
                     LPRADRLMAALDGYGSDAIGVVYDVANAVFAREDPVAGLRRVKARLALVHLSDTGTDS
                     YRHAPVGTGAVPFDRVARALDDIGYRDVAMLEIVSPAPDADIPASIDRLLADGAWRAL
                     AAAA"
     gene            3648..4076
                     /locus_tag="VGB88_03145"
                     /pseudo
     CDS             3648..4076
                     /locus_tag="VGB88_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(3962..4432)
                     /locus_tag="VGB88_03150"
     CDS             complement(3962..4432)
                     /locus_tag="VGB88_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9646469.1"
                     /translation="MFQSAKILRTLVAVLALSIAAGCAKQASPPPTQAAAASGPELAG
                     AWYQIYFDTNSTEINARGQTIIKTVAHVAANAGPTRVTVIGRTDRVGAPPDNMALAHR
                     RANVVRDALITAGVPAAQIDTTWTGEGDQTVATSDDLAEPRNRVVDVTVVKQSP"
     gene            complement(4477..4719)
                     /locus_tag="VGB88_03155"
     CDS             complement(4477..4719)
                     /locus_tag="VGB88_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX9646470.1"
                     /translation="MKRITLAAVLLFPLFALGACGQTTGERAISGGGIGAGVGAVGGL
                     LVGAPLEGALIGGAVGAATGALTDEQQISLGRPIWK"
     gene            complement(4957..5267)
                     /locus_tag="VGB88_03160"
                     /pseudo
     CDS             complement(4957..5267)
                     /locus_tag="VGB88_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5243..5944
                     /locus_tag="VGB88_03165"
     CDS             5243..5944
                     /locus_tag="VGB88_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646471.1"
                     /translation="MIYLDSPATGVNGVAIGIALLIDSNGEMGIYRYATINPSVGMRA
                     EYPSRSAWQSPLSPLMYCWELLGGNRPGAIGRESVMGTRGTFARGAGLAGAIIALQMV
                     STAPAAAQEAATPAEAAAMRVRVVQTEMMVAALTCDLHAQYNTVIEVHGSELKSHAAV
                     LKSMFRRLHGGAAQPRLDSYVTELANDASMRSIRARRSFCEAAGEMFKTALDDGMTLT
                     EVSGRLEQQAALPTP"
     gene            complement(6071..7189)
                     /locus_tag="VGB88_03170"
     CDS             complement(6071..7189)
                     /locus_tag="VGB88_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9646472.1"
                     /translation="MQMYTRNTLAKLSATALAAVAMSSPATAEDMTYDSSGAYIFGSA
                     GIHQTQRSKLSGGGLDTSVTHDPGFAALAGAGYDFGNIRLELEGGYRRADVDDVGGVD
                     GNGRVRTYSVMGNVFYDLDFGGGFKPYIGGGLGAAHVRAKASAPVGPFAGIGIDDNST
                     RPALQGGAGVAIALAERLDLTIDYRYLFAGPWRLEFSTNAGQDVRTRLNDHAIFVGLR
                     FSLNPPPAPPPPAPAPVAEPRPAPPAPPPPPAPQPQPPAAVEPEPVREFLVFFDWDRS
                     DLTSEAREIIAAAATEAKRISPVRIVATGHADRSGPSPYNVGLSQRRADSVKAELIRQ
                     GISANEIATIARGETDPLVSTADGVREPRNRRVLIVLE"
     gene            7415..8917
                     /locus_tag="VGB88_03175"
     CDS             7415..8917
                     /locus_tag="VGB88_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9646473.1"
                     /translation="MSGARDYDVVVVGGGGAGLAAAIEARSLGRSVALLEKNPALGGT
                     TGLSVGSITATNTPHQLAKGIKDSPAHHFDDMPGFAGPLANRDNPALRRLLTDNVPDT
                     FRWLLGLGLTFYGPMPEPPHRKPRMHTVLPNSRAYVWHLARRARALGAEVFTGAAVEE
                     LMRHDGRVTGVVARIDGARETFRARGGVVLASGDYSASGALKERYMSPAHAKVDPINP
                     ASTGDGHALALALGARVVNGDLSLGPELRFVPPARETLVRRLPPHRALARFMAWSMDH
                     MPERLLRPFVMSFLTTWLAPSPRLFAEGAVLVNRNGRRFADELDAPALALPDQPGKIG
                     YILFDAAVARTFSAWPHFISTAPGIAYAYLADYRRNRTDITHSAPTLAGLAARLGMDG
                     AALEASVAEHNAALDGGRPRLAEPPFWALGPVRSAIIFTDGGLAVSERLEVLGADDRP
                     IPGLYAAGSAGQGGLLLEGHGHHLGWAFTSGRIAARHAAWPVTSKDLPAD"
     gene            complement(8931..9092)
                     /locus_tag="VGB88_03180"
     CDS             complement(8931..9092)
                     /locus_tag="VGB88_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9646474.1"
                     /translation="MLDGEGVIELAGARRVVRRHDVIFIPPGVEHSIMNTGLDNLVFL
                     VITVPPTDE"
BASE COUNT         1371 a         3192 c         3106 g         1424 t
ORIGIN      
        1 gccagcaacg cggtgacgtc gatgtcgttg gtcgcggtgc ggatgcgcgc ggcgttctcg
       61 ggcgatgtgg tgatgcgctg caagtcgttg aaccagtcga cctgcgccgg cgtgccatcg
      121 ggcagaaaca gggtcgcgaa cagctggcgg atcgccgggt tgtcctggcc ccaggagtgg
      181 cggatcaagg taatcatggc ctcggcctgg tcgatgtccg cctgactgcc gcgcttgcgc
      241 cggccgcggg cgaagccgcc atagaggacc agcttgctga cgcgctcggg gtggcggacg
      301 gcataggcga tcgagaccgc gcagccctgc gagatgccga gcagcgggaa gcggtcgagg
      361 cggcaggcat cgaccaccgt ctcgaggtcg ctgacgaacg cctcgaacga gatgtcctcg
      421 acggtccagt cggacaggcc gttgccgcgc gcgtcgtagc ggaccagcat gtgatcgtgc
      481 gcgagctcag ccagcaggtg gcgccagatc gggctggtcc agtcatattc gaggtggttg
      541 agccagttcg ccgtcttgac cagcggcggg ccggcgccgg cgaccgcata ggcgatcgag
      601 gtgccgtcgc gcgcgctgca gaaccggatg tcctgatgac cggcggccgc ggcatcggcg
      661 cgcggccggc ccgccgcgcc gacggcgtag acgcgcaccg gcttggcgat gttcttgacc
      721 ggccgctcgc cgagaaaatc gaagccgcag tcgagcttgc cctcgaccgc gtcatggacc
      781 gcgcccgaga cgcagatgcc gcccgcctcg gcgagcgcct ccaggcgcgc ggcgatgttg
      841 accccgtcgc cgtagacggt gccgtcggcc tcctcgatca cgtcgccgag gttgacgccg
      901 atgcgcaagc gcatgcgccg ctcgggcgcg agctcgacgt tgcgcccctc gacggcctgt
      961 tgaatctcga ccgccgccgc gaccgcctcg accgcgctcg cgaagtcggc gagcagcgca
     1021 tcgcccggcg cgttcaccac ccgcccgccg tggcgcgcga ccagtcccgc aatgacctcg
     1081 cggcaggtct tgagcgcggc caccgtcgcc gcctcgtcgg cctgcatcag gcgcgaatag
     1141 ccgaccacgt ccgccgacag gatcgccgcc agcttccgct tgacgcccgg ctgcgttgcc
     1201 accgcaatgc ccgttcgcgt gcgtggaccg gtggccgttt cagccgttga acaccaccag
     1261 gcgctcctcg gtcatctcgc ggatcgcgta gtgcgggccc tcgcggccga gcccgctgtc
     1321 cttgaccccg ccataggcga gctggtcggt gcgccaggtc gaggtgccgt tgatgatcac
     1381 gccgccggtg cggatgcgcc ggatcgcgct cagcgcgacc gcgagcgagg cgctgaacag
     1441 cccgcactgg agcccgtagc ggctggcgtt gatgcgggcg aacgcctggt cgatgtcgtc
     1501 atagggaatc acgctgacca cgggcccgaa cacctcctcg cacaccacct tcatcgacgc
     1561 ctcgacgtcg gcgagcacgc acggcgcata ttgcgcgccg acgcgatggc cgccggcgag
     1621 ccgccgggcg ccgccggcga gcgcctcggc gacccacgat tcgacgcgcc gcgcggcgtc
     1681 ctcgtcgatg cacgtgccga cgtcggtggc ctcctccagc gggtcgccga ccttgagcgc
     1741 gtccaccgcc gcgaccaggc gatgcgtgaa cgagtccgcg accgcgcggt ggacgaagac
     1801 cttctgcacc gatatgcagc tctggccggc aaggcgcatc gcgttgcgcg cgcacatcgt
     1861 cgccgcggcg tcgatgtcgg catcggcatg gacgatggtc gggccgtcgc cgccgagttc
     1921 gagcgcgacc cgcttgagcc cgcacgcggc gcggatgcgc gcgccggtgg cactcgagcc
     1981 ggtgaagctg atcatgtcga ccttggggtg ggcaaccagc gccggcccgg ccgcctcgcc
     2041 gtagagcacg ttcagcagcc ccgccgccgc gcccgcgtcg acaaacagct cggcgagctt
     2101 gtggacgctg agcggcgcct gcggcggcgc cttcagcacc accgcattgc cggccgcgat
     2161 cgccggcgcg accttgtggc aggcgaggtt gaacggcgcg ttgaacgggg tgatcgcggc
     2221 gactacgccg acgggcacgc gcagcgtgaa cgcgattcgg cccgcgccca tcgcgctcgc
     2281 ctcgagcggc acctgctcgc cggtgatgcg caccgcttcc tcggccgaca gcgcgatggt
     2341 ctcggccgag cgctcgacct cggccgcgca gtcgcggatc gccttgccgg tctcgcgcgc
     2401 catcgcccgg gcaatgtcgc cggtgcgctc ggccagcaag gtgcccaccc ggcgcagcag
     2461 cgccgcgcgc tgatagcccg gcaccgccgc cgcccggtcc ttggccgcga ccgccgcctc
     2521 gaccgcggcg tcgacgtcgg cggcggtgga caccggtacc gttgccacca cctcgccgcg
     2581 ataggggtcg cggtcctggt aagagtcggc gccgtcgcgc caggcgccgc cgatcagcat
     2641 gcgcaccgcc tgcgcctggc cctgggcccg cttgtgcgcg gcaaccgcct ggtcgtacat
     2701 tgccctctcc tttttccgca cgcgccgctt gcccgtgcgg ccacaattta gaaagctaag
     2761 tttcgcgttc cgccgctgtc aactcggggc cgggcgatgg tcgaaagcgt gcgctatttc
     2821 gggatcaaca cctattccta catctacagc cacgccgccg ggccgtgcct ggaggcgctg
     2881 gcggcgcgcg gccacaccca tttcgagctg atgatgtatc ccgggcacgc ttggccccag
     2941 gacatggacg cgcgtgcccg ccaacgcctc cggcgcgcca tcgacgccgg cgggctcacg
     3001 ctctgtaccc tcaacatgcc caacatcgat gtcaacatcg ccgctgccgc gcccgcgatg
     3061 cgcgagctca gccgcccgca tctcaagcgc gtgatttcgc tcgccggcga tctcggcgcc
     3121 gccggggtgg tggtcggccc gggcaagccg aacccgctgt ttccgggccc ggtggaccag
     3181 atgacgggct ggttctacca ggcgctcgat gtcctggtgc cgctggcgga gcgcgcgggc
     3241 acccgggtgc tggtcgagaa catgccgttc gcgttcctgc cccgcgccga ccggctgatg
     3301 gctgcgctcg atggttacgg cagcgacgcg atcggggtgg tctacgacgt cgccaatgcg
     3361 gtgtttgcgc gcgaggaccc ggtggcgggg ctgcgccggg tcaaggcgcg gcttgcgctc
     3421 gtgcacctgt cggacaccgg caccgacagc taccggcacg ccccggtcgg caccggcgcg
     3481 gtgccgttcg accgcgtcgc gcgcgcgctt gacgacatcg gctatcgcga cgtggcgatg
     3541 ctcgagatcg tctcgcccgc gccggacgcc gacattccgg cgagcatcga ccggctgctc
     3601 gccgacggcg cctggcgcgc gctcgcggcg gcggcatgac ggcgatggcg gcggggcggc
     3661 cttacgacgt ggtcgtggtc ggcggcggcg gcgccgggct cgccgcggcg atcgaggcgc
     3721 gcagcctcgg gcgcagcgtc gcgctcttgg agaagaaccc ggcgctgggc ggcaccaccg
     3781 gcgacaagcg cggcctagga ccatttcgac ctgtcgtcct gcgacggtcc actcgcgctt
     3841 tgatcacgaa ctctgcatcg cggtgtgcga gcaggcggct cgagtggcga gtgccgcagc
     3901 gcattcgcga gtgcgatcgg cgccgaccgt ctgaaccgac attacatgcc gccgatcacg
     3961 gtcaaggaga ctgcttgact acggtgacat cgaccacacg gttgcgaggt tcagcgagat
     4021 cgtccgaggt agcgaccgtt tggtcgcctt cgccagtcca ggtcgtgtcg atttgagcgg
     4081 ccggcacccc ggcggtgatg agcgcatcgc ggaccacgtt cgcccgtcga tgcgccagtg
     4141 ccatgttgtc gggtggggcg ccgacgcgat cagtccggcc gatcaccgtc acgcgcgtcg
     4201 ggccggcgtt tgctgcgaca tgggccacgg tctttatgat cgtctgccct ctcgcgttga
     4261 tctcagtgct gttggtatcg aagtaaatct ggtaccaggc gccagccagc tcaggaccgc
     4321 tcgcggcggc agcctgtgtc ggcggcggtg atgcttgttt cgcgcatcca gctgctatgg
     4381 aaagcgccaa gacagcaacg agcgttcgta ggattttcgc agattgaaac atttttaatt
     4441 ccatcgtctg ccgacattgc cttggacgtg tgcggcctac ttccatatcg gtcggccgag
     4501 actgatctgt tgctcatcgg tgagggcgcc ggtggcagcg ccgacggcac caccgatcag
     4561 cgcgccctca aggggagcgc cgaccagcaa tccgccgacc gcaccgactc cggcgccgat
     4621 tccgccgccg cttatggcgc gttcaccagt ggtctggccg cacgcgccga gcgcgaatag
     4681 agggaacaaa aggaccgccg ccaaagtaat tcgtttcatg atttgcctcc acgcgggtag
     4741 ttcatccatt ccccctcgcc caggtcgacc aatgccgcag cacgcatcac gacaaccggt
     4801 cttgtctcat agcttcttgc ctaaccttgg caccaacgct tattattcct agggccgcta
     4861 tatcaagtat gcattgatct gcatcaagag ggggcgacaa agccccagcc ggagtggaac
     4921 gtggaatcgt gagcgccggc cgacctcagc gccacgaccg acaatgctgc gcatgacccg
     4981 atcgagcgac agccggtagg cgccgttcac gtcctcatag ccggccaggc atccaaacac
     5041 ggactgccgg aacaggcaag taacgtgtgt tgggtattca gacccgtacg ccggtcgcgc
     5101 aggtccgaag tcgcaatctc ggtcaagccg agggcagcag cgaacgcccg gaacagcacc
     5161 agcccgccgt cggaacttag cttggcgcca tggaaatgca gcttgatccg ccggtcgaaa
     5221 ctgatcttga aagagccgct ctttgattta cctcgattca cccgcaacag gcgtgaacgg
     5281 cgtggcgatt ggcatcgccc ttctcattga ttccaatggc gaaatgggga tctatcggta
     5341 tgcaactatc aacccatctg tggggatgcg ggctgaatat ccgagccgat ccgcttggca
     5401 atcgccgtta tcgccgttaa tgtattgttg ggaattgctg ggcggtaatc ggcctggagc
     5461 catcggtcgg gagagcgtga tggggacgag ggggacgttc gcgcgggggg ccggcctggc
     5521 cggtgcgatc atcgcgttgc agatggtgtc gacagcaccc gcggccgcgc aggaagcggc
     5581 cacgccggcc gaagcggcgg cgatgcgggt gcgcgttgtc cagacggaaa tgatggtcgc
     5641 ggcgctgacc tgcgacctgc atgcacaata caacacggtc atcgaagtcc acggcagcga
     5701 gctgaaaagt cacgcagccg ttctgaagag catgttccgg cgcctgcacg gtggtgcagc
     5761 ccaacccagg ctcgacagct atgtgaccga acttgccaac gacgcctcga tgcgcagcat
     5821 ccgcgcgcgc cgctcgttct gcgaggctgc gggcgagatg ttcaagacgg cgcttgacga
     5881 cggcatgaca ctgacggagg tcagcggcag gctggagcag caggccgccc ttcccacgcc
     5941 ctgatccgcc ggcgcaggat ccgctgcccg gcctgccaca gcgtgcgaaa cgcaaaaaag
     6001 cggcggcccg ctactcacgg gccgccgccg gtcatttagt tggcgcgtcg gcgcgcgccg
     6061 tggtgccgat ctattcgagc acgatcagga cgcggcggtt gcgcggctcg cgcacgccgt
     6121 ccgccgtcga cacgagcgga tcggtctcgc cccgggcgat cgtcgcgatc tcgttcgccg
     6181 agatgccttg ccggatcagc tcggccttca cgctgtcggc gcgccgctgg gaaagcccca
     6241 cattgtacgg cgaggggccc gaccggtcgg cgtgaccggt ggccacgatc cgcaccgggc
     6301 tgatgcgctt ggcctcggtc gcggcagccg cgatgatctc gcgcgcctcg gacgtcaggt
     6361 cggagcggtc ccagtcgaag aagacgagga attcgcgcac cggttccggc tccacggcag
     6421 cgggtggctg cggttgcggc gcgggcggcg gcgggggcgc gggcggcgcc ggcctcggct
     6481 ctgccacggg ggccggcgcg ggcggcggcg gggccggcgg cgggttcagc gagaaccgca
     6541 ggcccacgaa tatcgcgtgg tcgttcaatc gggtacgaac gtcttggccc gcgttggtgc
     6601 tgaactccag cctccacggg cccgcgaaca ggtagcggta gtcgatagtc aagtccagac
     6661 gctcggcaag cgctatcgcc acgccggcgc cgccctgcaa agcgggccgg gtgctgttgt
     6721 cgtcgatccc gatcccggcg aacggaccga caggcgcgga ggctttcgcc ctgacatgcg
     6781 cggcgccgag gccgccgccg atgtagggct tgaacccgcc accgaagtcg agatcataga
     6841 agacgttgcc catcacgctg tacgtgcgga ctctgccgtt gccatccaca ccgccgacat
     6901 cgtccacgtc ggcccggcga tagccgcctt cgagctcgag tcggatgttg ccaaaatcgt
     6961 agccggcgcc cgccagggcg gcgaaacccg ggtcgtgcgt gaccgacgta tcgaggccgc
     7021 cgccgcttaa cttggaacgc tgggtctgat ggatgccggc cgaaccgaag atgtaggcgc
     7081 cagacgaatc gtacgtcatg tcctcggcgg tggccggaga gctcatggca acggcggcca
     7141 gggcggtcgc cgagagcttt gcgagggtgt tccttgtgta catctgcatt tttttcctcc
     7201 gtttgattgg gttaatttcc cccaatgcag catgcaagac atggatcccg cccatcatgc
     7261 ggcgtaaatt gctaactagc acaggtcagg gtccgatccg cgtgtctccg ccgtcttctg
     7321 cacaacaatc aagcaactgt tgcaatttta tcacaattgt atctagcatc ctgggcgccg
     7381 accccgcctg gcgctggcgc gcggaggcgc gctgatgtcg ggcgcgcgcg actacgacgt
     7441 ggtcgtggtc ggcggcggcg gcgccgggct cgccgcggcg atcgaggcgc gcagcctcgg
     7501 gcgcagcgtc gcgctcttgg agaagaaccc ggcgctgggc ggcaccaccg ggctgtcggt
     7561 gggctcgatc accgccacca acacgcccca tcagctggcc aagggcatca aggactcgcc
     7621 cgcgcatcat ttcgacgaca tgcccgggtt cgccgggccg ctcgccaacc gcgacaatcc
     7681 ggcgctgaga cggctcttga ccgacaacgt gcccgacacc ttccgctggc tgctcggcct
     7741 cggcctcacc ttctacgggc cgatgcccga gccgccgcat cgcaagccgc gcatgcacac
     7801 cgtgctgccc aactcgcgcg cctacgtctg gcacctcgcg cgccgggcgc gcgcgctcgg
     7861 cgccgaggtc ttcaccggcg cagccgtcga ggagctgatg cggcacgacg gacgggtgac
     7921 cggcgtcgtc gcccgcatcg acggcgcgcg cgagacattt cgcgcccgtg gcggcgtggt
     7981 gctggcaagc ggcgactaca gcgcgagcgg cgcgctgaag gagcgctaca tgtcgccggc
     8041 acatgccaag gtcgacccga tcaacccggc cagcaccggc gacgggcacg cgctcgcgct
     8101 ggcgctcggc gcgcgggtgg tcaacggcga cctgtcgctc ggccccgagc tccgcttcgt
     8161 gccgccggcg cgcgagaccc tggtccgccg cctgccgccg caccgcgcgc tggcgcgctt
     8221 catggcctgg tcgatggacc acatgcccga gcggctcttg cgcccgttcg tgatgagctt
     8281 cctcaccacc tggctcgcgc cctcgcccag gctgttcgcg gagggcgcgg tgctggtcaa
     8341 ccgcaacggc cggcgcttcg ccgacgagct cgacgcgccc gcgctcgccc ttcccgacca
     8401 gccgggcaag atcggctaca tcctgttcga cgccgccgtg gcgcggacgt tctcggcctg
     8461 gccccacttc atctcgaccg cgcccggcat cgcctacgcc tacctcgccg actaccggcg
     8521 gaaccgcacg gacatcaccc acagcgcgcc gacactcgcc ggcctcgccg cgcggctcgg
     8581 catggacggg gccgcgctcg aggcctcggt cgcggagcac aacgcggcgc tcgacggcgg
     8641 ccgccctcgg ctcgccgagc cgccgttctg ggcgctcggc ccggtccgaa gcgcgatcat
     8701 cttcaccgac ggcgggcttg cggtgagcga gcgactcgaa gtcctcggcg cggacgatcg
     8761 cccgattccc ggcctctacg ccgcgggctc ggccggccag ggcgggctgt tgctcgaagg
     8821 ccacggccac cacctgggct gggcgttcac ctcgggccgc atcgccgccc gccacgccgc
     8881 gtggcccgtg acctccaagg acctgccggc ggactgaggc gcggcaggcc ctattcgtcc
     8941 gtcggcggca ccgtgatgac caggaacacg aggttgtcga ggccggtgtt catgatcgag
     9001 tgctcgacgc cgggcgggat gaagatcacg tcgtggcggc ggaccacgcg gcgcgcaccc
     9061 gcgagctcga tcacgccctc gccgtcgagc acg
//
LOCUS       DASWHU010000090         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2620400, whole genome shotgun sequence.
ACCESSION   DASWHU010000090 DASWHU010000000
VERSION     DASWHU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620400"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..811)
                     /locus_tag="VGB88_03185"
     CDS             complement(2..811)
                     /locus_tag="VGB88_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9646475.1"
                     /translation="MTARFRTFAARGFAAFAWIALAACSGGDPDPYVERPVEDLYNEA
                     MDQMLAGNYQPAARGFEEVERQHPYSVWATKAELMAAFSFYQNSSYDDAVIAAQRFIE
                     LHPGNPDAAYAHYLVGLSYYEQISDVGRDQRMTELALDSFEEVSKRYPDSRYARDASL
                     KISLVRDHLAGKEMEVGRTYQDLEFYLAAINRFRNVISNYQTTSHVPEALLRLTECFL
                     ALGVQGEAQAAAAVLGHNFPGSAWYADAYELLQERELEPRLSESSWLARLF"
     gene            919..1740
                     /gene="hisN"
                     /locus_tag="VGB88_03190"
     CDS             919..1740
                     /gene="hisN"
                     /locus_tag="VGB88_03190"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9646476.1"
                     /translation="MASAPAGDALADLLAFADTLADAARAVLVRHFRSDLAVDHKDSA
                     SPRGQPVTVADRDAEAAIRALIEARYPDHGIVGEELGSRHPDADWQWLIDPIDGTRAF
                     IAGLPLFGTLIGLGHLGRPVLGVIDQPVLGERFVGSRAGAFLNGAPIRTRACPRLERA
                     VFALTDPDMMAGPAERAAYRTLRRGAAVTQLGGNCYAYAMLAAGAIDLVVEADLKPWD
                     IYALVPVVEAAGGVIADWSGGPAAAASRVIACGDAALYDQVQPLLAGAAPADDAA"
     gene            complement(1737..2531)
                     /gene="pcaD"
                     /locus_tag="VGB88_03195"
     CDS             complement(1737..2531)
                     /gene="pcaD"
                     /locus_tag="VGB88_03195"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEX9646477.1"
                     /translation="MTDTIHANGIDIAYRLDGPQAAPLVTLSNSLASTMAMWDAQVPA
                     LTPDWRVLRYDTRGHGGTSAPPAPYRMETLAADLIGLWDALGVARSAFVGLSLGGMIG
                     QAIGIDHAHRVSALVLCDTLARWPQGAGTIWDGRIRVVQTQGVKALVDGTIERWFTAD
                     FRASGSPEIARIGAMIGATPAPGYAGCSYAIKGIDFLDRLKAISVPTLVMVGADDPAT
                     PPSAAREIQQKIPGAKLVVIENAAHLANVEQPKAFNRALRDFLDAL"
     gene            2614..>3434
                     /locus_tag="VGB88_03200"
     CDS             2614..>3434
                     /locus_tag="VGB88_03200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9646478.1"
                     /translation="MERESMDYDVVIVGAGPAGLACAIRLKQLCAEHDAELGVCVLEK
                     GSEVGSHILSGAVLEPRALDELIPDWRERDAPVRTAVAEERFMVLSERGGFAIPTALL
                     PAAMRNHGNYIVSLGNLCRWLAGQAEALGVEIYPGFAAAEVLYGKAGAVTGVATGELG
                     VTKAGEHGANWQPGVELHGKYTVFAEGCRGSLGKELKRRFALDAGADPQTYGIGLKEL
                     WEVEPARHRTGLVMHTVGWPLDRATYGGSFVYHLDEALVAIGFVVGLDYRNPYLDP"
BASE COUNT          474 a         1208 c         1247 g          505 t
ORIGIN      
        1 gctagaacag tcgcgccagc cacgagcttt cgctcagccg cggctccagc tcgcgctcct
       61 gcagcagctc gtaggcgtcg gcgtaccacg cgctgccggg aaaattgtgg ccgagcacgg
      121 ccgcggccgc ctgcgcctcg ccctgaacgc cgagcgcgag aaagcattcg gtcaggcgca
      181 acagcgcctc gggcacgtgg ctggtggtct gatagttgct gatcacgttg cggaaccggt
      241 tgatcgccgc caggtagaac tcgaggtcct ggtaggtacg gccgacctcc atctccttgc
      301 ccgccaggtg gtcgcggacc agcgagatct tgagcgaggc gtcgcgcgcg taacgcgagt
      361 cgggatagcg cttggagacc tcctcgaacg aatcgagcgc gagctcggtc atgcgctggt
      421 cgcgcccgac gtcggagatc tgctcgtaat agctgagccc gacaagataa tgggcgtagg
      481 ccgcgtccgg attgcccggg tgaagctcga tgaagcgctg ggcggcgatg accgcgtcgt
      541 cgtaggagct gttctggtag aacgagaatg ccgccatcag ctccgccttg gtcgcccaca
      601 ccgagtaggg gtgctggcgc tcgacctcct cgaagccgcg ggcggcgggc tggtagttgc
      661 cggcgagcat ctggtccatg gcctcgttgt agaggtcctc gaccggccgc tcgacatagg
      721 ggtcggggtc gccgcccgag cacgcggcga gcgcgatcca ggcgaaggcg gcgaagccgc
      781 gcgccgcgaa ggtccggaag cgagctgtca tgaccattgc actgcgcgcg gagtatagca
      841 tggcgggtga agccgcctag cgccgacgcc gcagccgccg cggttgaccg ggcgtgccgg
      901 ccctggcata tgcgcgccat ggcctcagcc cccgccggcg acgcgctcgc cgacctgctc
      961 gccttcgccg acaccctcgc cgacgccgcg cgcgccgtcc tggtcaggca tttccgcagc
     1021 gacctcgcgg tcgatcacaa ggactccgcc tcgccgcgcg gccagccggt gacggtcgcc
     1081 gaccgcgacg ccgaggcggc gatccgcgcc ttgatcgagg cgcgctaccc cgaccacggc
     1141 attgtcggcg aggagctggg gagccggcac ccggacgccg actggcagtg gctgatcgac
     1201 ccgatcgacg gcacccgcgc gttcatcgcc gggctgccgc tgttcggcac cctgatcggc
     1261 ctcggccacc tgggccggcc ggtgctcggc gtgatcgacc agccggtgct gggcgagcgc
     1321 ttcgtcggca gccgcgcggg ggcgttcctc aacggcgcgc cgatccgcac ccgggcgtgc
     1381 ccgcgcctgg agcgggcggt gttcgcgctc accgaccccg acatgatggc cggccccgcc
     1441 gagcgcgccg cctatcggac cttgcgccgg ggcgcggcgg tgacccagct cggcggcaac
     1501 tgctacgcct atgccatgct cgccgccggc gccatcgacc tggtggtcga ggccgacctc
     1561 aagccgtggg acatctacgc gctggttccc gtggtcgagg ccgcgggcgg ggtgatcgcc
     1621 gactggtcgg gcgggccggc ggcggcggcg agccgggtga tcgcctgtgg cgacgcggcg
     1681 ctctacgacc aggtccagcc gctgctcgcc ggcgccgccc ctgccgacga cgccgcctag
     1741 agcgcgtcga ggaagtcgcg cagcgcccgg ttgaacgcct tcggctgctc gacgttggcg
     1801 aggtgcgcgg cgttctcgat caccaccagc ttggcacccg gaatcttctg ctggatctcg
     1861 cgcgccgccg acggcggcgt cgccgggtcg tcggcgccga ccatgaccag cgtcggcacc
     1921 gagatcgcct tcagccggtc gaggaagtcg atgcccttga tggcatacga gcagccggcg
     1981 tagccgggcg cgggggtggc gccgatcatg gctccgatgc gcgcgatctc gggcgagccg
     2041 gaggcgcgga agtcggccgt gaaccagcgc tcgatggtgc cgtcgaccag cgccttgact
     2101 ccttgcgtct ggaccacgcg gatgcgcccg tcccagatcg tgccggcacc ttgcggccag
     2161 cgcgcgagcg tgtcacacag taccagcgcg ctgacccggt gcgcgtggtc gatgccgatg
     2221 gcttgcccga tcatgccgcc gagcgacagg ccgacgaagg ctgagcgcgc cacaccgagc
     2281 gcgtcccaca ggccgatcag gtcggcggcg agcgtctcca tcctgtacgg cgccggcggc
     2341 gcgctggtgc cgccgtggcc gcgggtgtcg tagcgcagca cgcgccagtc gggcgtgagc
     2401 gccggaactt gcgcgtccca catggccatg gtcgaggcca ggctgttgct caaggtcacc
     2461 agcggcgcgg cttgcgggcc gtcgaggcgg taggcgatgt cgatgccgtt ggcgtggatg
     2521 gtgtcggtca tgcgggtcct ccctggtccc cgtttgcaga cgcggccgtc gcggccgtgg
     2581 tatcacggcc cgcgagacta gcccggaggc gagatggaac gcgagtcgat ggactacgac
     2641 gtggtcatcg tcggcgccgg ccccgccggg ctcgcctgcg cgatccggct caagcagctg
     2701 tgcgccgagc acgatgccga gctcggcgtg tgcgtgctcg agaagggctc ggaggtcggc
     2761 tcccatatcc tgtcgggcgc ggtgctcgag ccgcgggcgc tcgacgagct gatcccggac
     2821 tggcgcgagc gcgatgcgcc ggtgcgcacc gcggtcgccg aagagcgttt catggtgctg
     2881 tccgagcgcg gcggattcgc gattcccacc gcgctgctgc cggcggcgat gcgcaaccac
     2941 ggcaactaca tcgtcagcct cggcaacctg tgccgctggc tcgccggcca ggccgaggcg
     3001 ctcggggtcg agatctatcc cggattcgcc gccgccgagg tgctgtatgg caaggccggc
     3061 gcagtgaccg gcgtcgccac cggcgagctg ggcgtgacca aggcgggcga gcacggggcg
     3121 aactggcagc cgggagtcga gctgcacggc aagtacaccg tgttcgccga aggctgccgg
     3181 ggcagcctgg gcaaggagct caagcgccgc ttcgcgctcg acgccggcgc cgatccgcag
     3241 acctacggca tcggcctcaa ggagctgtgg gaggtcgagc ccgcccgcca ccgcacgggc
     3301 ctggtcatgc acaccgtcgg ctggccgctc gaccgcgcca cctatggcgg ctcgttcgtc
     3361 taccacctcg acgaggccct ggtcgcgatc ggcttcgtcg tcggcctcga ctaccgcaac
     3421 ccctatctcg accc
//
LOCUS       DASWHU010000091         7109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2620745, whole genome shotgun sequence.
ACCESSION   DASWHU010000091 DASWHU010000000
VERSION     DASWHU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620745"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGB88_03205"
     CDS             <1..550
                     /locus_tag="VGB88_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX9646479.1"
                     /translation="AARREAVARIAGALAETRAPWPEARLALAGEVEGWLDEMPAVDA
                     EQHLRARLGAGRRADAEAGRAGCGPHRSDLAVTHLESGLPAAQCSTGEQKALLISIVL
                     ANARLHAARRGTTPLVLLDEVAAHIDRVRRAALFEALAALGAQAWLSGTDAQLFEGLR
                     GRAQFFVLADGRARAAAGAARW"
     gene            544..2997
                     /gene="gyrB"
                     /locus_tag="VGB88_03210"
     CDS             544..2997
                     /gene="gyrB"
                     /locus_tag="VGB88_03210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX9646480.1"
                     /translation="MVSTHIQNPPRADAGEYGAESIKVLRGLDAVRKRPGMYIGDTDD
                     GSGLHHMVYEVVDNSIDESLAGYCDRIEVTLNVDGSVTVTDNGRGVPTDIHEEEGVSA
                     AEVIMTHLHAGGKFDHNAYKVAGGLHGVGVSVVNALSETLEMTVWRAGREHFMRFHDG
                     EPEAPLAVVGEADSRTGTKVTFLPSKRTFTNTEFDFARLEHRLRELAFLNSGVRIALT
                     DKRLLEPLTVEMHYEGGIEAFVAFLDRNKQALHSPPIMLSGERDGIIVDAALQWNDSY
                     HENVLCFTNNIPQSDGGTHLAGFRAALTRTINGYAGETGLLKREKVQLTGDDTREGLT
                     CVLSVKVPDAKFSSQTKDKLVSSEVRPVVEGVVADRLGQWFEEHPSEARAIVGKVIEA
                     AAAREAARKARELTRRKGALDITTLPGKLADCQERDPAKSELFIVEGDSAGGSAKQAR
                     NRAFQAILPLRGKILNVERARFDKMLSSAEIGTLIAALGTGIGGDDFSVERIRYHKVI
                     IMTDADVDGAHIRTLLLTFFYRHMREIIQRGYLFIAQPPLFKVTRAGKSVYKKDEREL
                     DDYLIDEGLRDARLLVGRPSAPEVRAGADLRELVVRARDQAAAMRALARREHRGLIEQ
                     AAVAGALDPELIRAPESAAAAADYIVQRLNALAGPGEDGWTRRIADDGALLFRLEARG
                     EVSNFRIDEPLVQSNEARRLTAAREELQALYAHPAVLEAKDRRIEPVRSPIDLFEAVL
                     ELGRKGTSIQRYKGLGEMNPEQLWETTLDPNVRTLLQVKISHAGDADDLFSTLMGDLV
                     EPRRNFIQEHALDVANLDV"
     gene            3055..4911
                     /locus_tag="VGB88_03215"
     CDS             3055..4911
                     /locus_tag="VGB88_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX9646481.1"
                     /translation="MGRSRGDRRRAKAGGTLRAAHRRPQATTARRGLLARFARWAAYW
                     GAVAGIWLALAAGAAVAWYARDLPDPAAAAARPRLAGITVEAANGIVLANYGEVYGEH
                     LDTAELPGALVEAVVATEDRRFFRHFGVDPWGLARAAVANLGAGRLVQGGSTITQQIA
                     KNVFLTPERSVKRKVQELLLALWLERRFTKHELLTIYLNRVYLGAGAYGVDAAARRYF
                     GKSARDLTLAEAAMIAGLPKAPSRYAPTRDLALARARAAVVLEAMVAAGSLAAGEAAR
                     AKAAPATVTSATVAAGGSRYFADWVVDQLPDYVGPAARDLIVVTTLSPWAQRAGERAV
                     ADALDGAGRDLGARQAALVAMTPDGGVRAMVGGRAYRDSQFNRVTQARRQPGSAFKIA
                     VYAAALEAGWSPDDMVADSPIRLAGWSPSNYGEAYLGDITLRRAFALSSNSVAVQLSE
                     RVGRRHVIEAARRLGIGAPLPDHPSLALGVGELSPLELTAAYATVANGGRGVIPYGIV
                     EIRDRAGRVLYRRTGSGPGRALAAPVAAALDGLLRAAVAEGTGKRAALPGPAAGKTGT
                     SQDFRDAWFVGYRPDLVAGVWLGNDDGAPMRNVTGGGLPAALWHDFMTRTAP"
     gene            complement(4928..5617)
                     /locus_tag="VGB88_03220"
     CDS             complement(4928..5617)
                     /locus_tag="VGB88_03220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX9646482.1"
                     /translation="MARTDSPLLGALPIRRRTSARARRLSLKLDAATGMVELVIPQGC
                     DARKADRFVAGNLGWISARLAELPPRIPFAHGAELPVLGRWLRLHHAARAARAPARRN
                     GVLVIGGPGHQLDARVRAWLRETARMALYQRAHDHAAEVGRRVSGVRIGDPRSRWGSC
                     SPGGVLSFSWRLVLAPPHVLDYVVAHEAAHLRYMSHGPRFWALVRRLDPDFESAREWL
                     ARDGAQLHRYG"
     gene            complement(5604..6074)
                     /locus_tag="VGB88_03225"
     CDS             complement(5604..6074)
                     /locus_tag="VGB88_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX9646483.1"
                     /translation="MTRPPPASRGAILVPIWLLWAALAHAAEVEVSDGWARASAGAGG
                     VAAAYLTLTNRGGETALTGVETPRAGHAGLHATSEEGGVMRMRMVDRIPLAPGASVTL
                     QPGGLHVMLMGLAEPLVEGDRLALTLIFEDGGRIDTEVPVLAPGASGSEAHGAH"
     gene            complement(6099..6704)
                     /locus_tag="VGB88_03230"
     CDS             complement(6099..6704)
                     /locus_tag="VGB88_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9646484.1"
                     /translation="MTAKALAFAFAIAATVAFAFVLAWGPDAPVREAGRTGTPAIGGP
                     FTLVDGDGRTVTDADFRGRTLVVYFGYSFCPDVCPTTLAIMARAIDLLGADGEAVVPV
                     FITVDPARDTPAVVGAYVANFHPRMVGLTGSAEQVEAAAKAYKVYYAKAETEAAAGSD
                     EYLMDHSSIIFLIGPDGRYLDHFTGGTGADQLAAGLRAHLG"
     gene            6804..>7109
                     /locus_tag="VGB88_03235"
     CDS             6804..>7109
                     /locus_tag="VGB88_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9646485.1"
                     /translation="MARASLLILSLLCLALAAPAVAGAREDSRLLTGARGQLIAVVEA
                     ALEAGADLEARDAGGRTALIWAAFHDHAHMLEFLLARGADVNAQDAHGRTALIWAATA
                     "
BASE COUNT          968 a         2645 c         2596 g          900 t
ORIGIN      
        1 ggcggcacgc cgagaggcgg tggcgcgcat cgccggcgcg ctcgccgaga cgcgggcacc
       61 gtggcccgag gcccgcctcg cgctcgccgg cgaggtcgag gggtggctcg acgagatgcc
      121 cgcggtcgat gccgagcagc acctgcgcgc gcgcctcggc gccggccggc gcgccgatgc
      181 cgaggccggg cgtgccggct gcggcccaca tcgcagcgac ctcgccgtca cccaccttga
      241 atccgggcta cccgcggcgc agtgctcgac cggcgagcag aaggcgctct tgatctcgat
      301 cgtgctggcc aacgcgcgcc tgcatgcggc gcggcgcggc accacgccgc tggtgctctt
      361 ggacgaggtc gcggcccata tcgaccgggt gcgccgtgcg gcgctgttcg aggcgctcgc
      421 ggcgctcggc gcccaggcgt ggctcagcgg caccgatgcc cagctgttcg agggcttgcg
      481 gggccgcgcc cagttcttcg tcctcgccga cggccgggcg cgcgcggccg cgggtgccgc
      541 gcgatggtga gcacgcacat ccagaacccg ccgcgcgccg atgccggcga gtacggcgcc
      601 gagtcgatca aggtgctgcg cggcctggac gcggtgcgca agcgcccggg catgtatatc
      661 ggcgataccg acgacggctc gggcctgcac cacatggtct acgaggtggt cgacaactcg
      721 atcgacgagt cgctggccgg ctattgcgac cgcatcgagg tcacgctcaa cgtcgatggc
      781 tcggtgaccg tcaccgacaa cggccgcggc gtgccgactg acatccacga ggaggagggc
      841 gtgtcggcgg ccgaggtgat catgacccac ctgcacgccg gcggtaagtt cgaccacaac
      901 gcctacaagg tcgcgggcgg gttgcacggg gtcggcgtgt cggtggtcaa cgcgctgtcg
      961 gagaccctcg agatgaccgt ctggcgcgcc ggccgggagc atttcatgcg cttccacgac
     1021 ggcgagcccg aggcgccgct cgccgtggtc ggcgaggccg attcgcgcac cggcaccaag
     1081 gtcaccttcc tgccgtccaa gcgcaccttc accaacaccg agttcgattt cgcccggctc
     1141 gagcaccgct tgcgcgagct cgcgttcctg aactccgggg tgcgcatcgc gctcaccgac
     1201 aagcgccttt tggagccgct gacggtcgag atgcactacg agggcggcat cgaggcgttc
     1261 gtcgccttcc tcgaccgcaa caagcaggcc ctgcacagcc cgccgatcat gctcagcggc
     1321 gagcgcgacg gcatcatcgt cgacgccgcg ttgcagtgga acgacagcta ccacgagaac
     1381 gtgctgtgct tcaccaacaa catcccgcag agcgacggcg gcacccatct ggcgggcttc
     1441 cgcgcggcgc tgacgcgcac catcaacggc tatgccggcg agaccgggct gctcaagcgc
     1501 gagaaggtgc agctgaccgg cgacgacacc cgcgaggggc tgacctgcgt gctgtcggtc
     1561 aaggtgcccg acgccaagtt ctcgtcccag accaaggaca agctggtcag ctccgaggtc
     1621 cgcccggtgg tcgagggcgt ggtcgccgac cgcctcggcc agtggttcga ggagcacccg
     1681 agcgaggccc gcgccatcgt tggcaaggtg atcgaggcgg cggccgcgcg cgaggcggcg
     1741 cgcaaggcgc gcgagctgac gcggcgcaag ggcgcgctcg acatcaccac gctccccggc
     1801 aagctcgccg actgccagga gcgcgacccg gccaagagcg agctgttcat cgtcgagggc
     1861 gattccgccg gcggctcggc caagcaggcg cgcaaccgcg cgttccaggc gatcctgccg
     1921 ctgcgcggca agatcctcaa cgtcgagcgc gcgcgcttcg acaagatgct gtcctcggcc
     1981 gagatcggca ccctgatcgc ggcgctcggc accggcatcg gcggcgacga cttctcggtc
     2041 gagcgcatcc gctaccacaa ggtcatcatc atgaccgacg ccgacgtcga cggcgcccac
     2101 atccgcaccc tgctgctgac cttcttctac cggcacatgc gcgagatcat ccagcgcggc
     2161 tacctgttca tcgcccagcc gccgctgttc aaggtcaccc gcgccggcaa gtcggtctac
     2221 aagaaggacg agcgcgagct cgacgactac ctgatcgacg agggcttgcg ggacgcgcgg
     2281 ctgctggtcg ggcggccgag cgcgccggag gtgcgcgccg gcgccgacct gcgcgagctg
     2341 gtggtgcgcg cgcgcgacca ggcggcggcg atgcgggcgc ttgcccggcg cgagcatcgc
     2401 ggcctgatcg agcaggcggc ggtggccggg gcgctcgacc ccgagctgat ccgcgcgccc
     2461 gagagcgcgg cggcggccgc ggactatatc gtccagcgcc tgaacgcgct cgccggcccc
     2521 ggcgaggacg gctggacgcg ccgcatcgcc gacgacggcg ccctgctgtt caggctcgag
     2581 gcgcgcggcg aggtgtcgaa ctttcgcatc gacgagccgc tggtgcagag caacgaggcg
     2641 cgccggctga ccgcggcgcg cgaggagctg caggcgctgt acgcgcaccc ggcggtgctc
     2701 gaggccaagg accggcgcat cgagccggtg cgctcgccca tcgacctgtt cgaggcggtg
     2761 ctcgagcttg gccgcaaggg caccagcatc cagcgctaca aggggctcgg cgagatgaac
     2821 cccgagcagc tgtgggagac caccctcgac cccaacgtgc gcacgctgct ccaggtcaag
     2881 atcagccacg ccggcgacgc cgacgacctg ttctcgaccc tgatgggcga cctggtcgag
     2941 ccgcggcgca acttcatcca ggagcacgcg ctcgacgtcg ccaacctcga cgtctgagcc
     3001 cgccggccgc ctcgcggctg gtcgccggcc cgccccgcgc cccatattga gggcatgggg
     3061 cggagtcggg gcgacaggcg gcgggcgaag gcgggcggca ccctcagggc cgcacaccgc
     3121 aggccgcagg cgaccaccgc ccgccgcggg ttgctcgccc gcttcgcccg ttgggccgcc
     3181 tattggggcg cggtcgccgg catctggctc gcgcttgccg ccggcgcggc cgtcgcctgg
     3241 tacgcccgcg acctgcccga ccccgcggcc gccgccgccc gcccgcgcct cgccggcatc
     3301 accgtcgagg ccgccaacgg catcgtgctc gccaactacg gcgaggtcta cggcgagcac
     3361 ctcgacacgg ccgagctgcc gggcgcgctg gtcgaagccg tggtcgcgac cgaggatcgc
     3421 cgcttcttcc gccatttcgg cgtcgatccg tgggggctcg cgcgcgccgc ggtggccaac
     3481 ctcggcgccg gccggctggt ccagggcggc agcacgatca cccagcagat cgccaagaac
     3541 gtgttcctca cgcctgagcg cagcgtcaag cgcaaggtcc aggagctgct gctggcgctg
     3601 tggctggagc ggcgcttcac caagcacgag ctgctgacca tttacctcaa ccgggtctat
     3661 ctgggcgccg gcgcctacgg cgtcgatgcc gccgcgcgcc gctacttcgg caagtccgcg
     3721 cgcgacctca ccctggctga ggcggcgatg atcgcgggcc tgcccaaggc gccgagccgc
     3781 tatgcgccga cccgcgacct ggcgctggcg cgcgcccgcg ccgcggtggt gctcgaggcc
     3841 atggtcgccg ccggcagcct cgccgcgggc gaggcggcgc gggccaaggc cgccccggcc
     3901 acggtcacca gcgcgaccgt cgccgcgggc gggtcgcgct acttcgccga ctgggtggtg
     3961 gaccagctgc ccgactacgt cggcccggcg gcgcgcgacc tgatcgtcgt caccacgctc
     4021 agcccgtggg cgcagcgggc gggggagcgc gcggtggccg atgcgctcga cggcgccggc
     4081 cgcgacctcg gcgcgcgcca ggcggcgctg gtggcgatga ccccggacgg cggcgtgcgc
     4141 gccatggtcg gcggccgcgc ctatcgcgat agccagttca accgcgtcac ccaggcgcgg
     4201 cgccagcccg gatcggcgtt caagatcgcg gtctacgccg ccgcgctgga ggccggctgg
     4261 tcgcccgacg acatggtcgc cgacagcccg atccggctcg ccggctggag cccgagcaac
     4321 tacggcgagg cgtatctcgg cgacatcacc ctcaggcgcg cgttcgcgct gtcgagcaac
     4381 agcgtcgcgg tccagctcag cgagcgggtc gggcgcaggc atgtgatcga ggccgcgcgc
     4441 cggctcggca tcggcgcgcc gttgccggac cacccgagcc tcgcgctcgg cgtcggcgag
     4501 ctgagcccgc tcgagctcac cgccgcctac gccacggtcg ccaacggcgg gcgcggcgtc
     4561 atcccctacg gcatcgtcga gattcgcgac cgcgccgggc gggtgctcta ccggcgcacc
     4621 ggctcaggtc ccgggcgggc gctcgcggca ccggtggcgg cggcgctcga cggcctgttg
     4681 cgcgccgcgg tcgccgaggg caccggcaag cgcgcggcgc tgcccggccc ggccgccggc
     4741 aagaccggca ccagccagga cttccgcgac gcgtggttcg tcggctaccg gcccgacctg
     4801 gtcgccgggg tgtggctcgg caacgacgac ggtgcgccca tgcgcaatgt caccggcggc
     4861 ggcctgcccg cagccctgtg gcacgacttc atgacccgca ccgcgccgtg agcgccgcgc
     4921 ggccggctca gccgtaacgg tggagctgcg cgccgtcgcg cgccagccac tcgcgcgcgc
     4981 tctcgaagtc ggggtcgagc cggcggacca gcgcccagaa gcgcggaccg tggctcatat
     5041 atctgagatg cgcggcctcg tgcgcgacga cgtagtcgag cacgtggggc ggcgccagca
     5101 ccagccgcca cgagaacgac agcacgcccc ccggcgagca gctgccccag cggctgcgcg
     5161 ggtcgccgat gcgcacgccg gagacgcggc ggccgacctc ggcggcatgg tcgtgggcgc
     5221 gctggtagag cgccatgcgc gcggtctcgc gcagccaggc acgcacccgg gcgtcgagct
     5281 gatggcccgg gccgccgatc accagcacgc cgttgcggcg cgccggcgcg cgcgcggcgc
     5341 gggcggcgtg gtgcaggcgc agccacctgc ccagcaccgg cagctcggcg ccatgggcga
     5401 acgggatgcg cggcggcagc tcggccaggc gggcgctgat ccagcccagg ttgccggcga
     5461 cgaaccggtc ggccttgcgc gcgtcgcagc cctgcgggat gaccagctcg accatgcctg
     5521 tggcggcgtc gagcttgagg ctcagccgcc gcgcccgcgc cgatgtccgg cgccggatcg
     5581 gcagcgcgcc gagcagcggg ctgtcagtgc gcgccatgag cctcggaacc gctggcgccc
     5641 ggcgccagca caggcacctc ggtgtcgatg cgcccgccgt cctcgaagat gagcgtgagc
     5701 gccagccggt cgccctcgac cagcggctcg gcgagaccca tcagcatgac gtggagcccg
     5761 cccggttgca gggtcacgct ggcgcccggg gcgagcggga tgcgatcgac catgcgcatg
     5821 cgcatgaccc cgccctcctc gctggtggcg tgcaggccgg cgtggccggc gcgcggcgtc
     5881 tcgaccccgg tcagcgccgt ctcgccgccg cgattggtga gggtgagata ggcggcggcg
     5941 acaccgcctg cgcccgcgct cgcccgcgcc cagccgtcgc tgacctcgac ctcggcggcg
     6001 tgcgccaggg ccgcccagag gagccatatc gggaccagga ttgcgccacg cgaggccggc
     6061 ggcggtcggg tcatggtcgg gctcccccag gtcgtcgatc agccgagatg ggctcgtagt
     6121 ccggcggcca gctgatcggc gccggtgccg ccggtgaagt ggtcaagata gcgcccgtcg
     6181 ggcccgatca ggaagatgat cgagctgtga tccatcaagt attcgtcgct gccggccgcg
     6241 gcctcggtct cggccttggc gtaatacacc ttgtaggcct tggccgccgc ctcgacctgc
     6301 tcggcgctgc cggtcagccc gaccatgcgc gggtggaaat tggcgacata cgcgccgacc
     6361 accgccgggg tatcgcgcgc cgggtcgacg gtgatgaaca ccggcaccac ggcctcgccg
     6421 tccgcgccca gcaggtcgat cgcccgggcc atgatcgcga gcgtggtcgg gcacacgtcg
     6481 gggcagaagc tgtagccgaa atagaccacc agggtgcggc cgcggaagtc ggcatcggtg
     6541 accgtccgcc cgtcgccgtc gaccagggtg aacggcccgc cgatcgcggg cgtgccggtc
     6601 cgcccggcct cgcgcaccgg cgcgtccggg ccccaggcca ggacgaacgc gaacgcgacg
     6661 gtggccgcga tcgcgaacgc gaaggcgagg gccttcgccg tcacccgcgc ctccccgcgc
     6721 gcctgccccg gccggcgttc gcacgcgggc ggggctggtc tatagtcgcg cggccccctc
     6781 gaccgtgccg gagacggatc gccatggccc gtgcctcgct gctcatcctg tcgctgctat
     6841 gccttgcgct ggcggcgccc gcggtcgccg gcgcgcgcga ggattcgcgc ctgctcaccg
     6901 gcgcccgcgg ccagctgata gcggtggtcg aggccgcgct cgaggccggc gccgatctcg
     6961 aggcgcgcga cgcaggcggg cggacggcgt tgatctgggc agcgtttcat gaccatgcgc
     7021 acatgctcga atttctgctc gctcgcggcg ccgacgtcaa tgcacaggac gcgcatggcc
     7081 gcaccgcgct gatctgggcc gcgaccgcc
//
LOCUS       DASWHU010000092        13145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3359052, whole genome shotgun sequence.
ACCESSION   DASWHU010000092 DASWHU010000000
VERSION     DASWHU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359052"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..735
                     /locus_tag="VGB88_03240"
     CDS             166..735
                     /locus_tag="VGB88_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9646486.1"
                     /translation="MSHIAQEIIEANHRYAQSFGEKSKLAMPPARRFAILTCMDARLD
                     PAAFAGLAEGDAHVIRNAGGRASDDAIRSLVISYKLLGTREWFVIHHSDCGMETFTND
                     VMRTLLASSLETAKFDGGAWRDLGKGPGSPEAGYIDWLTIADQRQSVTDDVHRIHRHP
                     LVPKDIPITGYIYDVRSGELIQVEGAAAP"
     gene            778..1413
                     /locus_tag="VGB88_03245"
     CDS             778..1413
                     /locus_tag="VGB88_03245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9646487.1"
                     /translation="MALQLGDTAPNFVQDSTLGPIDFYDWAGDSWVVLFSHPKDFTPV
                     CTTELGRVAKLKGEFDRRDVKVIGLSVDAVPDHKAWAGDIADTQGAALNFPLVADADR
                     RVSDLYGMIHPKANDTLTVRSVFVIDPKRKVRLTITYPASTGRNFDEILRVIDSLQLT
                     DRYSVATPVDWRRGDDVIIVPSLGAEEARKKFPAGWTEIRPYLRVVADPSA"
     gene            complement(1373..2440)
                     /locus_tag="VGB88_03250"
     CDS             complement(1373..2440)
                     /locus_tag="VGB88_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9646488.1"
                     /translation="MAGAARLSAAGAPVAFPRFVPRAPWWGGDLQTVRNTMLGARDDL
                     ARWPGERVSFAMPDGSGDRLSAMLHRPDGEACGPLVVILHGLTGCEDSVHVRASAAYF
                     LARGWPALRLNLRGAGPSRKSCRGLYHAGRTDDLRAVLDVLAAEPGAGDIVVLGYSLG
                     GNVALKLLGEAGAAPLPGRVRAAASVSAPIDLAATWRNFRRPRNRLYQAWLLARMKEE
                     AVAPGAAVSEAERRAVAGARTIFEFDDRFVAPRNGFAGADDYYARCSSAHVLARIGLA
                     TLLISARDDPWVPVAPYMAHDWQATPALTPLLPRRGGHVGFHGRGGRAAWHDLCAERF
                     FAAALAHPSRRTGPRPRASTA"
     gene            complement(2419..3282)
                     /locus_tag="VGB88_03255"
     CDS             complement(2419..3282)
                     /locus_tag="VGB88_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9646489.1"
                     /translation="MLERKAHTLDDGGGRRRLSYLEAGAGQDGVPVLCVHGMTRNARD
                     FERLAPALARTRRVICVDLPGRGDSDRLADSTRYQIPTYAGDLLALLDALGIAEVDWV
                     GTSLGGMIGMAVAGAEDSAVRGVIRRLVLNDIGPFIPKAALARIGEYVVRTWRFESLA
                     HAEAHVRKAYAPFGLRSDSDWRRLTELSVQPDADGGLIQNYDPAIGDDMRERAPTDID
                     LWPAWDRIRCPVLVLRGADSDVLPAEVAAEMTRRGPGAELAAFAGIGHAPALLDDDQI
                     SVVAEWLARPA"
     gene            3381..4544
                     /locus_tag="VGB88_03260"
     CDS             3381..4544
                     /locus_tag="VGB88_03260"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9646490.1"
                     /translation="MPESTPPPGPLSGVTVLDLTRVLAGPYCTMVLSDLGARVIKVEP
                     PAGDDARAYGPWLDGRSTYFMSLNRGKESVALDLKDDADRALFERLLARADVLVENFR
                     PGTMAKLGYGWEALQARHPRLIYAACSGFGHTGPLARRPAYDLVVQGMGGIMSLTGHD
                     GGPPTRVGTAIGDIAAGMFTAIGVNAALFDRARTGRGQFIDIAMLDCQLAILENAIAR
                     YFTTGETPGPSGARHPAIAPFECYRSGDGHIILAAGNETLFRRLCAALGVPELADDPR
                     FRTNPERMSHVEALKAALEAVLARGTTAQWLEKLEAAGVPCGPLNSVDMAVSQPQAQA
                     RNMVVSLDDPRAGPLRIAGNPIKLSAYPDPATRGPVPELGEHRARIERELDEG"
     gene            4725..7052
                     /locus_tag="VGB88_03265"
     CDS             4725..7052
                     /locus_tag="VGB88_03265"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9646491.1"
                     /translation="MRWLARILVVVAVLAVAAAGAAWLVLERTLPKVDGEITLAGIGG
                     PIEVVRDAAGVPHVFAATERDAYFALGFVHAQDRLWQMELARHAAAGRLGELFGTLAL
                     PHDRYFRTLGFGRVAARIVDTLPAAARALLDAYAAGVSAYIASHRGPPGVEFALLGQE
                     PAPWRPEDSILVVKIMATDLAGNAAGELLRAKLASHLSAAQVAALWPPYPGDQAPPPP
                     GHAALDEELVGRALALLPPARPPGIGSNNWAVAPGRSASGAAILANDPHLGLAVPAVW
                     YLAHLHADGLDVVGGTIPGIPAVIVGRNRSLAWGVTNTGPDAQDMFVVADDDTLTART
                     ETIALKGADAVDIVVRESPFGPIVSDATERYAAVTGGRALALSWTALETRDDTVQAAF
                     ALARARTVDDGMAALADFDDPQQNFLLADGGGHIGFVAAGRVPVRAGTDGWTPTDASG
                     GGAGWRGYLPYDALPRARDPQSGVLFTANQRIVPDDYPSFITRDWAEPYRARRIAEML
                     AARERHSVADFMAMQTDIVSLAAREMLPLLLAVPLSARGERLRPQLAAWDSAMAADRP
                     EPLIFHAWLRALMVRLLADELGDDFAGYQGHRPLVIEAILDHDHAWCDDVRTDAAEDC
                     PSQVAAALDDALDWLEARYGADPARWRWGAAHQATSRHALLSSLPVIGGWFGVVRAHG
                     GGPHTVMQANTRPGDAAAPFAEVHGASLRAIFDLADPDSTRLIVHAGQSGHWWSRHYD
                     DLADRWAAGAYLTVPLGRAAVEAAAAHRLTLRPAS"
     gene            7049..8269
                     /locus_tag="VGB88_03270"
     CDS             7049..8269
                     /locus_tag="VGB88_03270"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX9646492.1"
                     /translation="MTVTIDDIRAARARLGDDVVVTPCLVSRTLSAITGATVVLKFEN
                     HQFTASFKERGARNRLLQLDGAARRRGVIAVSAGNHAQGVAYHAQLLAIPAIIVMPRG
                     TPFIKIEQTSHFGPEVRLEGDSLAEAEDFARALAERERLTLIHPYDDEAIIAGQGTIA
                     LEMLEAAPDIDVLVVPVGGGGLIAGNAIAAKAVKPGIEVVGVETELYPAMYQALHGEP
                     VRAGGATIAEGIAVSRPGKLTLPVIRKLVAEVMVVPESEIERAVLLLLEIEKTVVEGA
                     GAAGLAALLARPERFKGRTVGLILSGGNIDVRLLANIVMRGLVREGRLERIMVEIADA
                     PGNLARVADIIGRAGANIVEVYHERAFTRMPIKTAALVVVMEIRNRDHGRAILEALVA
                     AGFPARPLELPAPG"
     gene            8316..8753
                     /gene="mscL"
                     /locus_tag="VGB88_03275"
     CDS             8316..8753
                     /gene="mscL"
                     /locus_tag="VGB88_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9646493.1"
                     /translation="MLSEFRAFALRGNAIDMAVGIIIGAAFGRIVSSLVNDVIMPPVG
                     LLLGGVDFTNLFVTLSAGSYPTLTEAQAAGAATINVGVFINAVIDFVIVAFAIFLVVR
                     QMNRLKRQPPPAEPTEKDCPFCLARIPIKATRCGHCTSELARA"
     gene            complement(8768..9538)
                     /locus_tag="VGB88_03280"
     CDS             complement(8768..9538)
                     /locus_tag="VGB88_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9646494.1"
                     /translation="MSDEDRRRFLKGLVALPLATVLAYPDLARAAAARLEPVAVDTRS
                     GRTAHGALALPDRVPAPAILLIHEWWGLNDQIKSVAQELAEEGYIALAADLYDGTVAT
                     TGEEAAALVGSFDSAAGLDKLAALVDWLKRRDDGTGKVGTIGWCFGGGWSLNVSLAAP
                     VDATVIYYGNVAKSPAQLASLSSPVLGHFAAQDARINTDMVAGFEAAMAAAGKTELSV
                     HWYDADHAFANPTGARYDADDARLAWSRTLDFFRRHLG"
     gene            complement(9663..10907)
                     /locus_tag="VGB88_03285"
     CDS             complement(9663..10907)
                     /locus_tag="VGB88_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9646495.1"
                     /translation="METVSTQCCIAGGGPAGMMLGLLLARAGVRVLVLEKHADFLRDF
                     RGDTIHPSTLAVIDELGWLDEFLSLPHQEIPEVTLLFGDTEISLADFRHLPTRCGFIA
                     FMPQWDFLEFLSAHAAAYPTFALRMSAEVTGLVEFGGEIRGVRARTAAGEMEVAAELT
                     VAADGRGSILRAQSGLRVCELGAPMDVLWFRLERTPGHADQPLGRFGAGEVLIMIDRG
                     AYWQCGYVIAKGTQESIRARGFDEFRRRVARFAPFLNDQLNALKGWDEVSLLTVRANR
                     LERWWRPGLLCIGDAAHAMSPVAGVGINLAIQDAVAAANLLAAPLARSAVTLDHLRGV
                     ERRRMWPTIVTQRLQSLIQDRVIAEALDENARLTPPLAFRLLDRYPVLRRLPGRAIGL
                     GIRPEHVRSLEAAQASISARDN"
     gene            complement(10925..12106)
                     /gene="chrA"
                     /locus_tag="VGB88_03290"
     CDS             complement(10925..12106)
                     /gene="chrA"
                     /locus_tag="VGB88_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX9646496.1"
                     /translation="MPRTPLVTLFLVFLKLGLTAFGGPVAHLGYYRREFVLRRGWLDE
                     QGFAELVAIGQFLPGPSSSQVGIAVGAQRAGLAGAILAWVGFTLPAAALMTLFAYGVG
                     HLDPARAGGWIAGLKIAALAVVANALLGMARSLTPDAPRAGLALAAAAVAVLLPGTAG
                     QLGAIGLGALAGTMTTPASEPPRHAPAGGIGRRTAIVCAVLFVALLVGLPVAAAVWQA
                     PWLTVAAGFFRAGSLVFGGGHVVLPLLESVVVPPGWVDRETFLAGYGAAQAIPGPMFA
                     ITAYLGAAMVPEPNGVAGGLLCLVALFLPGFLLVLAALPAWRAVSRIARLRGAMAGVN
                     AAVVGLLGAALYDPVFTSAVHAAPDLVIAAAGFVALARWRAPPWAVVVVLAAGRAALG
                     Q"
     gene            complement(12109..13038)
                     /locus_tag="VGB88_03295"
     CDS             complement(12109..13038)
                     /locus_tag="VGB88_03295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9646497.1"
                     /translation="MACAARLAGLVAATRPASLAPQAAWAETARRAYGFDGYRLLDRG
                     EIRREVASERYHGGLIEATSRHLDPLAYLAGLADAAQGQGARLYEESPAIRVAFGGRP
                     QVWTARGTVRARALVLAGNAYLGALVPELSARVMPVASYVCATEPLGEARARALIPNR
                     VAVCDDRAALDYYRLTGDGRLLFGGGASFAARTPNDLRRALRRRLLRTFPQLADVAIA
                     HAWSGLVAITRNRLPDIGRLAPNVYYAQGFSGQGLVLTGVAGRAIAEAITGRTDDLDL
                     LARIRHAPFPGGRLLRTPLLVAGRAWHHLKDLL"
BASE COUNT         1945 a         4793 c         4694 g         1713 t
ORIGIN      
        1 agcgggcgaa aaaaattctc ctgatcgtcc gcttccggag aacacaaacc taagaacagc
       61 gcgaacattc aaaagtcctt tcgcgggcgc acgacggcag ttgccaagca ctccggacaa
      121 tgcctatttg cgccgccaca agcacaattt cgccaggagt cgaccatgag ccacatcgcg
      181 caggagatca tcgaggccaa tcatcgctac gcgcagagct tcggcgagaa gtcgaagctc
      241 gccatgccgc cggcgcgccg gtttgcgatc ctcacctgca tggatgcccg cctcgacccg
      301 gcggcgttcg ccggcctcgc ggaaggcgac gcccatgtca ttcgcaacgc cggcggccgc
      361 gccagcgacg atgccatccg gtcgctggtg atctcgtaca agctgctcgg cacccgcgaa
      421 tggttcgtca tccaccacag cgactgcggc atggagacct tcaccaacga cgtcatgcgc
      481 acattgctgg cgagcagcct cgagaccgcc aagttcgacg gcggcgcgtg gcgcgacctc
      541 ggcaaggggc ccggctcgcc cgaggccggc tacatcgact ggctgaccat cgccgaccag
      601 cgccagtcgg tgaccgacga cgtgcacagg attcaccgcc acccgctggt gcccaaggac
      661 atcccgatca ccggctacat ttacgacgtg cgctcgggag agctgatcca ggtcgagggt
      721 gccgcggcac cgtgatctct tctgacgcca ttcgataggg aacgaaagga aaaaaccatg
      781 gccctgcagc tcggcgacac cgcgcccaac ttcgtccagg actcgacctt gggcccgatc
      841 gatttttacg actgggccgg cgactcctgg gtggtcctgt tctcgcatcc caaggacttc
      901 accccggtct gcaccaccga gctcggccgg gtagcgaagc tcaagggcga gttcgaccgg
      961 cgcgacgtca aggtgatcgg gctgtcggtg gacgcggtgc cggaccacaa ggcgtgggcc
     1021 ggcgacatcg ccgacaccca gggcgcagcg ctcaatttcc cgctcgtcgc cgatgccgac
     1081 cggcgggtct cggacctcta cggcatgatc catccgaagg ccaacgacac gctcaccgtg
     1141 cgctcggtgt tcgtgatcga ccccaagcgg aaggtgcggc tgaccatcac ctatccggcg
     1201 agcacggggc gcaacttcga cgagatcctg cgcgtcatcg actcgctgca gctcaccgac
     1261 agatatagcg tggcgacgcc ggtcgactgg cgccgcggcg acgacgtgat catcgtgccc
     1321 tcgctcggcg ccgaggaggc gcggaagaag ttccccgccg gctggaccga gatcaggccg
     1381 tacttgcgcg tggtcgcgga cccgtccgcc tagacgggtg cgcgagggcg gcggcgaaga
     1441 accgctcggc gcacaggtcg tgccaggcgg cgcgcccgcc gcggccgtgg aagccgacat
     1501 ggccgccgcg ccgcggcagc agcggcgtca gcgccggcgt cgcctgccag tcgtgggcca
     1561 tgtagggcgc gaccggcacc cagggatcgt cgcgggcgct gatcagcagg gtggcgaggc
     1621 cgatgcgggc gaggacgtgc gccgatgagc agcgcgcgta gtagtcgtcg gcgccggcga
     1681 agccgttgcg cggggcgacg aagcggtcgt cgaactcgaa gatcgtgcgc gcccccgcca
     1741 ccgcccgccg ctcggcctcg gagaccgcgg cgccgggcgc gaccgcttcc tccttcatgc
     1801 gcgcgagcag ccaggcctgg tagaggcggt tgcgcgggcg cctgaagttg cgccaggtcg
     1861 ccgcgaggtc gatcggcgcc gagaccgatg cggctgcgcg gacccgtccc ggcaggggcg
     1921 ccgcgccggc ctcgcccagc agcttgagcg cgacgttgcc gccgagcgag tagcccaaca
     1981 cgacaatgtc gccggcgccc ggctcggcgg cgagcacgtc gagcaccgcg cgcaggtcgt
     2041 cggtgcggcc ggcatggtag aggccgcgac aggacttgcg cgacggccca gcgccgcgca
     2101 ggttgaggcg cagcgccggc cagccgcggg cgagaaaata ggcggcgctc gcgcgcacgt
     2161 gcacgctgtc ctcgcaaccg gtgagcccgt gcaggatcac caccagcggc ccgcacgcct
     2221 cgccgtcggg gcgatggagc atcgcgctca gccggtcgcc cgagccgtcg ggcatggcga
     2281 agctcacccg ctcgccgggc cagcgggcga ggtcgtcgcg cgcgccgagc atggtgttgc
     2341 gcaccgtctg caggtcgccg ccccaccacg gcgcccgtgg cacgaagcgg ggaaaggcga
     2401 ccggcgcgcc ggcggcgctc aggcgggccg cgccagccat tccgccacca cactgatctg
     2461 gtcgtcatcg aggagcgccg gcgcatggcc gatgccggcg aacgcggcca gctcggcgcc
     2521 gggtccgcgg cgggtcatct cggccgcgac ctcggcgggc agcacgtccg agtcggcgcc
     2581 gcgcagcacc agcaccgggc aacgaatccg gtcccaggcc ggccacaggt cgatgtccgt
     2641 cggcgcgcgt tcgcgcatgt cgtcgccgat cgcgggatcg tagttctgga tcaggccgcc
     2701 gtcggcgtcg ggctggaccg acagctcggt cagccgccgc cagtcggagt cagagcgcag
     2761 cccgaaaggc gcataggcct tgcgcacatg ggcctcggcg tgcgccaggc tctcgaaccg
     2821 ccaggtgcgc accacgtact cgccgatccg cgccagcgcc gccttcggga tgaacgggcc
     2881 gatgtcgttg agcaccaggc ggcggatgac accgcgcacc gccgagtcct cggcgccggc
     2941 gacggccatg ccgatcatcc cgccgagcga ggtgccgacc cagtcgacct cagcgatgcc
     3001 gagcgcgtcg agcagcgcga gcaggtcgcc ggcataggtc gggatctggt agcgggtcga
     3061 gtcggccagg cggtcgctgt cgccgcgccc gggcaggtcg acgcaaatca cccgccgcgt
     3121 gcgcgccagc gccggcgcca ggcgctcgaa gtcgcgcgcg ttgcgggtca tgccgtggac
     3181 gcacagcacc ggcaccccgt cctggccggc gcccgcttcg agataggaca ggcggcgccg
     3241 gccgccgccg tcgtcgagtg tgtgcgcttt ccgttcgagc atccgccacc ggcccgccgt
     3301 ctgatcgccg cgcgcggctt gcgccggccc cggcgctggg ttatccgaac cgcacgcttc
     3361 cagaatcatg gaccgcgccg atgccagagt cgactccgcc gcccggcccg ctgtccgggg
     3421 tcaccgtcct cgacctcacc cgggtgctcg ccggcccgta ctgcaccatg gtgctgagcg
     3481 acctcggcgc gcgggtgatc aaggtcgagc cgccggcggg cgacgacgcg cgcgcctacg
     3541 ggccgtggct ggacgggcgg tcgacatact tcatgtcgct caaccgcggc aaggagtcgg
     3601 tcgccctcga cctcaaggac gacgccgacc gggcgctgtt cgagcgcctg ctcgcgcgcg
     3661 ccgacgtgct ggtcgagaac ttccgcccgg gcacgatggc caagctcggc tacggctggg
     3721 aggcgctgca ggcgcgccat ccgcgcctga tctacgccgc ctgctcgggc tttgggcata
     3781 ccgggccgct ggcccggcgc ccggcctatg acctggtcgt ccagggcatg ggcggaatca
     3841 tgagcctgac cggccatgac ggcgggccgc cgacccgggt cggcaccgcc atcggcgaca
     3901 tcgccgcggg catgttcacc gccatcgggg tgaatgccgc gctgttcgac cgcgcgcgca
     3961 ccgggcgcgg ccagttcatc gacatcgcca tgctcgactg ccagctcgcg atcctggaga
     4021 acgcgatcgc gcgctacttc accaccggcg agactcccgg gccgagcggc gcccgccacc
     4081 cggcgatcgc gccgttcgag tgctaccgga gcggcgacgg ccacatcatc ctggccgccg
     4141 gcaacgagac cctgttccgc cgcctgtgcg cggcgctcgg cgtgcccgag ctggccgacg
     4201 acccgcggtt tcgcaccaac cccgagcgca tgagccatgt cgaggcgctc aaggccgcgc
     4261 tcgaggcggt gctggcgagg ggcaccaccg cgcaatggct cgaaaaactt gaagcggccg
     4321 gcgtgccctg cggcccgctc aactcggtcg acatggcggt ctcccagccg caggcgcaag
     4381 cgcgcaacat ggtggtttcg ctcgacgacc cgagagccgg gccgctcagg atcgccggca
     4441 acccgatcaa gctgtcggcc tatcccgacc cggcgacgcg cgggccggtg cccgagctcg
     4501 gcgagcaccg cgcgcgcatc gagcgcgagc tcgacgaagg ctagaacatg gtccgctcag
     4561 gttggatcag cccccctcac ccttcccccg cgccccgcga ggcgggggga gagggttgcg
     4621 aagggttggc gcggcacctg ccgcgcctta ccccggagct gggtgagggg ggactgattc
     4681 atggccggcg gagaatgcat tagccgggcc cggacgcggc acccgtgcgc tggctcgccc
     4741 gcatcctggt ggtcgtcgcg gtgcttgccg tggcggccgc cggtgcggcg tggctggtgc
     4801 ttgagcggac cctgcccaag gtcgacggcg agatcacgct ggccgggatc ggcggcccga
     4861 tcgaggtggt gcgcgacgcc gccggcgtac cccacgtctt cgccgccacc gagcgcgacg
     4921 cgtacttcgc gctcggcttc gttcatgccc aagaccgcct gtggcagatg gagcttgccc
     4981 gacacgccgc cgccgggcgc ctcggcgagc tgttcggcac cctggcactg ccgcacgacc
     5041 gctatttccg caccctcggc ttcggccgcg tcgcggcgcg gatcgtcgac accctgccgg
     5101 cggcggcgcg tgcgctgctc gacgcctacg cggcgggggt gagcgcgtac atcgcgtcgc
     5161 atcgcgggcc gccgggagtc gagttcgccc tgctcggcca ggagccggcg ccgtggcggc
     5221 cggaggactc gatcctggtg gtcaagatca tggccaccga cctcgccggc aatgccgccg
     5281 gtgagctgct gcgggcgaag ctcgccagcc atctgagcgc ggcgcaggtg gcggcgttgt
     5341 ggccgcccta tcccggcgac caagcgccgc cgccgccggg ccacgccgca ctggatgaag
     5401 agctcgtcgg gcgcgccctc gccttgctgc cgcccgcgcg cccgccgggc atcggctcga
     5461 acaactgggc ggtggcaccg ggccgctcgg ccagcggcgc agcgatcctc gccaacgacc
     5521 cgcatctcgg cctcgcggtg ccggcggtgt ggtacctcgc ccacctgcac gccgacggcc
     5581 tcgacgtggt cggcggcacg attcccggca tcccggcggt gatcgtcggc cgcaaccgga
     5641 gcctcgcctg gggcgtgacc aataccgggc ccgacgccca ggacatgttc gtcgtggccg
     5701 acgacgacac gctcacggcg cgcaccgaga ccattgcgct caagggcgcc gatgcggtcg
     5761 acatcgtggt gcgcgagtcg ccgttcgggc cgatcgtctc ggacgcgacg gagcggtacg
     5821 cggcggtgac aggcgggcgg gcgctggcgc tgtcctggac cgcgctcgag acccgcgacg
     5881 acaccgtgca ggcggcgttc gcgctggcgc gcgcgcgcac cgtcgacgac ggcatggccg
     5941 cgctcgccga tttcgacgac ccgcagcaga acttcctgct cgccgacggc ggcgggcata
     6001 tcggcttcgt cgccgccggc cgggtgccgg tgcgcgcggg caccgacggc tggacgccga
     6061 ccgacgcgag cgggggcggc gcaggctggc gcggctacct gccctacgac gccctgccgc
     6121 gcgcgcgcga tccgcaatcg ggcgtgctgt tcaccgccaa ccagcggatc gttcccgacg
     6181 actacccgtc cttcatcacc cgcgactggg ccgagcccta tcgcgctaga cgcatcgccg
     6241 agatgctggc cgcgcgcgag cgccattcgg tggccgattt catggcgatg cagaccgata
     6301 tcgtgtcgct ggcggcgcgc gagatgttgc cgctgctgct ggcggtgccg ctgtcggcgc
     6361 gcggcgagcg cctgcgcccg cagcttgccg cgtgggacag cgccatggcg gccgaccgcc
     6421 ccgagccgtt gatcttccat gcctggcttc gcgcgctcat ggttcgcctg ctcgccgacg
     6481 agctcggcga cgacttcgcc ggctaccagg gccaccggcc gctggtgatc gaggcgatcc
     6541 tcgaccacga ccacgcctgg tgcgacgacg tgcgcaccga cgcggccgag gattgcccat
     6601 cgcaggtggc ggcggcgctg gacgacgcgc tcgactggct cgaggcgcgc tacggcgccg
     6661 atcccgcgcg ctggcgctgg ggcgcggcgc accaggcgac cagccgccac gccctgctgt
     6721 cgagcctgcc cgtgatcggc ggctggttcg gcgtggtgcg ggcccatggc ggcggcccgc
     6781 acacggtgat gcaggccaac acccgccccg gcgacgcggc ggcgccgttc gccgaggtcc
     6841 acggcgcgtc cttgcgcgcg atcttcgacc tcgccgaccc ggactcgacc cggctgatcg
     6901 tccatgccgg ccagtcggga cattggtggt cgcgccatta cgacgacctc gccgaccgct
     6961 gggccgccgg ggcgtacctg accgtgccgc tcggccgcgc cgcggtcgag gcggcggccg
     7021 cgcaccggct caccttgagg ccggcgtcgt gaccgtcacc atcgacgaca tccgcgcggc
     7081 gcgcgcccgg ctcggcgacg acgtggtggt gactccgtgc ctggtctcgc gcaccttgtc
     7141 cgcgatcacc ggcgcgaccg tggtgctcaa gttcgagaac caccagttca ccgcctcgtt
     7201 caaggagcgc ggcgcgcgca accgcctgct ccagctcgac ggcgcggcgc ggcggcgcgg
     7261 cgtgatcgcg gtctcggccg gcaaccacgc ccagggggtc gcctatcacg cccagctgct
     7321 cgccatcccg gcgatcatcg tgatgccgcg cggcaccccg ttcatcaaga tcgagcagac
     7381 cagccatttc ggccccgagg tgaggctcga aggcgacagc ctggccgagg ccgaggactt
     7441 cgcgcgcgcc ctcgccgagc gcgagcgcct gaccctgatc catccctatg acgacgaggc
     7501 gatcatcgcc ggccagggca ccatcgcgct cgagatgctc gaggcggccc ccgacatcga
     7561 cgtgctggtg gtgccggtcg gcggcggcgg cctgatcgcc ggcaacgcga tcgcggccaa
     7621 ggcggtcaag cccgggatcg aggtggtggg agtcgagacc gagctctacc cggcgatgta
     7681 ccaggcgctg cacggcgagc cggtgcgcgc gggcggggcg accatcgccg aaggcatcgc
     7741 cgtcagccgc cccgggaaac tcaccttgcc ggtgatccgc aagctggtcg cagaggtcat
     7801 ggtggtgccc gagtccgaga tcgagcgcgc ggtgctgctg ctgctcgaga tcgagaagac
     7861 cgtggtcgag ggcgccggcg ccgccgggct tgcggcgctg ctcgcccgcc ccgagcggtt
     7921 caaggggcgc accgtcggcc tgatcctgtc gggcggcaac atcgacgtcc gcctgctcgc
     7981 caacatcgtc atgcgcgggc tggtgcgcga gggccggctc gagcggatca tggtcgagat
     8041 cgccgatgcg cccggcaacc tcgcccgcgt cgccgacatc atcggccgtg ccggcgccaa
     8101 catcgtcgag gtctatcacg agcgcgcgtt cacccgcatg ccgatcaaga ccgcggcgct
     8161 ggtggtggtg atggagatcc gcaaccgcga ccacggccgc gcgatcctcg aggcgctggt
     8221 cgccgccggc tttcccgcgc gcccgctcga gctgccggcg ccgggctgag gccggggccg
     8281 gcgaagaccc tcaccctggg caagaggagg cttgaatgct gagcgagttt cgtgccttcg
     8341 cgttgcgcgg caacgccatc gacatggccg tcggcatcat catcggggcg gccttcggca
     8401 ggatcgtctc gtcgctggtg aacgacgtca tcatgccgcc ggtcggcctc ctgctcggcg
     8461 gggtcgactt caccaacctg ttcgtcaccc tgtcggcggg gtcgtatccg accttgaccg
     8521 aggcgcaagc cgcgggcgcc gcgacgatca atgtcggggt gttcatcaac gcggtgatcg
     8581 acttcgtcat cgtcgcgttc gcgatcttcc tggtggtgcg ccagatgaac aggctgaagc
     8641 gccagccgcc gccggcggag ccgaccgaga aggactgccc gttctgcctg gcgcgaatcc
     8701 cgatcaaggc caccaggtgc gggcactgca cgtccgagct ggcgcgggcg taggccgctg
     8761 ccgcggatca gccgaggtgg cggcggaaga agtcgagggt gcgcgaccag gcgaggcgcg
     8821 catcgtccgc gtcgtagcgc gcgccggtcg ggttggcgaa ggcgtggtcc gcgtcgtacc
     8881 agtggacgct gagctcggtc ttgccggccg ccgccatcgc cgcctcgaag ccggcgacca
     8941 tgtcggtgtt gatccgcgcg tcctgggcgg cgaaatggcc gagcaccggg ctcgacagcg
     9001 aggccaactg cgcgggcgat ttcgccacgt tgccgtagta gatcacggtc gcatcgaccg
     9061 gcgccgccaa cgacacgttg agcgaccagc cgccgccgaa gcaccagccg atggtgccga
     9121 ccttgccggt gccgtcgtca cggcgcttga gccagtcgac cagcgccgcg agcttgtcga
     9181 ggccggcggc ggagtcgaag ctcccgacca gcgccgcggc ctcctcgccc gtggtcgcga
     9241 cggtgccgtc atagaggtcg gcggcaaggg ctatatatcc ctcttctgca agctcttggg
     9301 cgaccgactt tatctgatcg ttgagccccc accattcatg gatcaagagg atcgccggcg
     9361 ccggcacgcg gtcgggcaaa gccagagcgc cgtgcgccgt gcgccctgaa cgggtgtcga
     9421 ccgcgaccgg ctcgaggcgg gcggcggcgg cacgcgccag gtccggatag gcgagaacgg
     9481 ttgccagcgg cagcgcgacc aggcccttga ggaaccgcct ccggtcctcg tccgacaccg
     9541 ccggcaagcg acctgccgtt gcgccggttt ggcaagattt caggtcacac ataccggtcc
     9601 tccccgagag tatgccatcg gttgcacggc gaagtgtagg acatggccgg cggcatggcc
     9661 cgtcaattgt cgcgcgccga gattgaggcc tgagcagcct caagcgaccg gacgtgctcg
     9721 ggccgtatgc cgagcccgat cgcgcgtccg ggcagccgcc gcaggaccgg gtagcggtcg
     9781 agcaggcgaa aggccaaggg cggcgtgagc cgcgcgttct cgtcaagggc ctcggcgatc
     9841 acccggtcct ggatcagcga ctggagacgc tgggtgacga tcgtcggcca cattcgccgc
     9901 cgttcgacgc cacgcagatg atcgagcgtc accgcggagc gcgcgagcgg ggcggcgagc
     9961 aggttggcag ccgccaccgc gtcctggatc gccaggttga tgccgacgcc ggcgaccggc
    10021 gacatcgcat gcgcggcatc gccgatgcac agcaggccgg gacgccacca gcgctccagg
    10081 cggttggccc ggacggtaag caggctcacc tcgtcccacc ccttgagcgc gttcaactgg
    10141 tcgttcagga acggcgcgaa gcgggcgacg cggcggcgga actcgtcgaa gccgcgcgcg
    10201 cggatgctct cctgcgttcc cttcgcgatc acgtagccgc actgccaata cgcgccgcga
    10261 tcgatcatga tcaggacctc gccggcgccg aaccgcccga gcggctggtc ggcgtgtccc
    10321 ggcgtccgct cgagcctgaa ccagagcacg tccatgggcg cgccgagctc gcacacgcgc
    10381 aggcccgact gcgcgcgcag gatcgagccg cgcccgtcgg cggcgacggt gagctccgcc
    10441 gcgacctcca tctctccggc cgcggtgcgc gcgcgaactc ctcgaatctc gccaccgaat
    10501 tcgaccagac cggtgacctc ggccgacatg cgcagcgcga aggtcgggta cgccgccgca
    10561 tgcgccgaca ggaactcgag aaagtcccat tgcggcatga acgcgatgaa gccgcatcgg
    10621 gtcggcaggt gacggaaatc ggcgagcgag atctcggtat cgccgaacaa cagcgtcact
    10681 tcgggaatct cctggtgcgg cagcgacagg aactcgtcga gccatcccag ctcgtcgatc
    10741 accgccaggg tcgagggatg aatggtgtcg ccgcggaagt ccctcaggaa gtccgcgtgc
    10801 ttttccagga ccaggacgcg cacgccggcg cgggccaaca gaaggccgag catcatgccc
    10861 gcgggtccgc cgccggcgat gcagcattgg gtcgagaccg tttccattcc cgccaccggc
    10921 tgtctcattg cccgagcgcc gcgcggccgg cggcgagcac gaccaccacc gcccagggcg
    10981 gcgcccgcca gcgcgcgagc gcgacgaagc ccgccgccgc gatcaccagg tcgggggcgg
    11041 cgtggaccgc cgaggtgaac accggatcgt acagcgccgc gccgagcagg ccgaccacgg
    11101 cggcgttcac ccctgccatc gcgccgcgca ggcgggcaat gcggctcacg gcgcgccacg
    11161 ccggcagcgc cgccagcacc agcaggaagc cgggcaggaa cagcgccacc aggcacagca
    11221 ggccgccggc gacgccattg ggctcgggca ccatggccgc gccgagatag gcggtgatcg
    11281 cgaacatcgg gcccgggatc gcctgcgccg cgccgtagcc ggcgaggaac gtctcgcggt
    11341 cgacccagcc cggcggcacg accaccgact cgagcagcgg cagcaccaca tggccgccgc
    11401 cgaacaccag cgagcccgcc ctgaagaagc cggcggcaac cgtcagccac ggcgcctgcc
    11461 agaccgccgc ggcaaccggc agcccgacca gcagggcgac gaaaagcacc gcgcaaacga
    11521 tcgcggtgcg ccgcccgatg ccgcccgccg gcgcgtgccg cggcggctcg cttgcgggcg
    11581 tcgtcatggt gccggccagg gcgccgagtc cgatcgcgcc gagctggccc gcggtgccgg
    11641 ggagcagcac cgcgaccgcc gcggcggcga gcgccaagcc ggcgcgcggg gcgtctggag
    11701 tgagcgaacg cgccatgccg agcaacgcgt tggcgactac cgccagcgcc gcgattttga
    11761 gcccggcgat ccagccgccg gcgcgcgccg ggtcgagatg accgactccg taggcgaaca
    11821 gggtcatcag cgccgccgcc ggcagggtga agccgaccca ggcgaggatc gcgccggcga
    11881 gccccgcgcg ctgcgccccg accgcgatgc cgacctggct cgacgacggc ccgggcagga
    11941 actgcccgat cgcgaccagc tcggcaaagc cctgctcgtc gagccagccc cggcgcagca
    12001 cgaactcgcg ccggtagtag ccgagatggg cgaccgggcc gccgaacgcg gtcagtccga
    12061 gcttgaggaa caccaggaac agcgtcacca acggcgtgcg cggcatcgtc acagcaggtc
    12121 cttcaggtgg tgccaggcgc ggccggcgac cagcagcggc gtgcgcagca gccgcccgcc
    12181 cgggaacggc gcgtggcgga tgcgcgcgag caggtcgagg tcgtcggtcc ggcccgtgat
    12241 cgcctcggcg atggcccggc cggcgacccc ggtcagcacc agcccctggc ctgagaaacc
    12301 ctgtgcatag tagacgttgg gcgccagccg tccgatgtcc ggcaggcggt tgcgggtgat
    12361 cgccaccagc ccggaccagg cgtgggcgat ggcaacgtcg gcaagctgcg ggaaggtgcg
    12421 gaggagtcgg cgccgcaacg cgcgccgcag gtcgtttggg gtgcgcgcgg caaagctcgc
    12481 cccgccgccg aacaagagcc ggccgtcgcc ggtcagccgg tagtagtcga gcgcggcgcg
    12541 gtcgtcgcag accgcgactc ggttcgggat cagcgcccgc gcccgcgcct cgcccaacgg
    12601 ttcggtggca catacatagc tcgctaccgg catcacgcgg gccgagagct cgggcaccag
    12661 cgcgcccaga taggcgttgc ccgccagcac caacgcccgt gcccgcaccg tcccgcgcgc
    12721 cgtccagacc tgtgggcggc cgccgaacgc gacgcggatt gcgggcgact cttcgtaaag
    12781 ccgcgcgccc tgcccttgcg cggcgtcggc gaggccggcg agataggcga gcgggtcgag
    12841 atggcgcgag gtcgcttcga tcaggccgcc gtgatagcgc tcgctcgcga cctcgcgccg
    12901 gatctcgccg cggtcgagca gccggtagcc gtcgaagccg taggcgcgcc gcgccgtctc
    12961 cgcccaggcg gcttgcgggg ccaggctggc gggcctggtc gcggcgacca ggccggccag
    13021 cctggccgcg caagccacct gggcggagac ggcgcggcgc gcctacggct tcgacggcta
    13081 ccggctgctc gaccgcggcg agatccggcg cgaggtcgcg agcgagcgct atcacggcgg
    13141 cctga
//
LOCUS       DASWHU010000093         6670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2999268, whole genome shotgun sequence.
ACCESSION   DASWHU010000093 DASWHU010000000
VERSION     DASWHU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999268"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VGB88_03300"
     CDS             <1..1311
                     /locus_tag="VGB88_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9646498.1"
                     /translation="ADQGYGEAAYQLGGMYERGIGVAADLAAAGGWYRQAATAGSVPA
                     AFRLGRMFAAGEGVEADFGLARDWYLQAATQGHAGAQNALGELYDDGLGGEEDVAEAL
                     RWYRAAAEQGDTDARANIALLHYLGRGVEQDYAAAFRGFRETANAGHVAAQYNLGVMF
                     ELGEGVPANLEQAVQWYLRAAEGGDADAQTRLGDMALAGRGVPQDQLAARNWFEDAAG
                     QGHAEAQVKLATLLYDGINGITDMGRALFWFREAARAGNAEAQYRLGMMAERGLGMAP
                     EPAQAAEWYALAAEAGHADAQFGLALMYELGAGVPANDQAAASWYAKAAALGHAGAQA
                     RLGALYRSGRGLARDVDQARGWLTRAAEQGEASALYNLGLLDANGEGGPRNPVRGLMW
                     LSLAADKGHEDAARDQARLAASMSEQEVDTARRLAGMWRDQHWR"
     gene            complement(1487..2782)
                     /locus_tag="VGB88_03305"
                     /pseudo
     CDS             complement(1487..2782)
                     /locus_tag="VGB88_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            2952..3608
                     /locus_tag="VGB88_03310"
     CDS             2952..3608
                     /locus_tag="VGB88_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9646499.1"
                     /translation="MIFTGLNHLAFITKDLEKTIRFYRDLLEMDLFAGIGHDGFRHYF
                     FKCGEGSIAFFEYDIAKPMEYDKFHGTPTDRPIGFDHVALTVPSRQDLFALKDRLEGA
                     GIEVTGPVDHGVMWSIYFFDPTNNLPLEASWDCMELLQAPAIEDDEPMAIAAEGAGPQ
                     PGHWPSPTRATPPDEMRAHSGNGYAMRESFLRRGLARNKPDLDIALAEGAAPDKQAAE
                     "
     gene            complement(3640..4764)
                     /locus_tag="VGB88_03315"
     CDS             complement(3640..4764)
                     /locus_tag="VGB88_03315"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9646500.1"
                     /translation="MTEQLVLADPSSIDFQALVRLAATRSEQGRTAVFQTISEFLVED
                     DWLHSDRERNLASDILRNLLGEVEVKLRLELACRLASRNDMPEALIADLASEPIEVAE
                     IVLRQSPVLSDQTLIEIIRERTEEHQLAIAQRRSLSTPVSGALIETDNDRVVTAVIEN
                     TGAQLTESMLRELVEDSRANETYQLALARRKDLPESLARKLCAWVAAPLRERICQRYH
                     IDPAVLDAEIAAAVDTAASAPAADLRRPSRARLIVETLAAKGSLDLRFLQGALFRGSV
                     DVFVEGLALLAGVDPRLVERALADDGPRTLAVVCKLASADHACFESLYRMSRGRNSLQ
                     ARSSHAERVRAQAAYQAVTVDQARKLADAWSAGKHAEPAG"
     gene            complement(4938..5642)
                     /locus_tag="VGB88_03320"
     CDS             complement(4938..5642)
                     /locus_tag="VGB88_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEX9646501.1"
                     /translation="MSCAPERFAVYFVPPAETALAAFGKAWFGAPHATAQRRAMADRL
                     GLAESVLAAITGAPRRYGFHATLKAPFRLRAACEPSALARATRRLAAATPRLAGPPLR
                     PALIGGFVALVPSVPCPALDRLADSCLTALDRFRAPLTSAEWRKRGPADLSAVERRHL
                     RRWGYPYVRDRFRFHMTLTGRLDRRLAERIRAALSADCLPYDGTPLAVDGLTLVRQSE
                     PGAMFESFGGFPLRTL"
     gene            complement(5639..6403)
                     /locus_tag="VGB88_03325"
     CDS             complement(5639..6403)
                     /locus_tag="VGB88_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646502.1"
                     /translation="MTALRLAVALALALVAGAPAAAVAAEDALARAESLYRDGAFADA
                     ADAGAALGTADGLALAARASNVRAIYLAGGEQRRALLSDAERFARRALAADRGHVEAA
                     LQLVVSLGEQARAMDAFQAFLAGLADESGRLLDGLARKADDNAYYHAVAGAWHGELVR
                     RGGAFLAGAFYGADLDEATRHFERALALAPASALIPTEYAKQLLALHGDTARARALLA
                     QATASPPKDAQRELVRREAARLLAELDTVAGAPSAP"
     gene            complement(6400..>6670)
                     /locus_tag="VGB88_03330"
     CDS             complement(6400..>6670)
                     /locus_tag="VGB88_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646503.1"
                     /translation="AAGYRLVPAEELRSLAAPGGGRRSLYRPDGLVAGSERLERFLLW
                     PMGIASAGAMRQFGTHATAFVGRRHFDDPRLFDDAFEWRRDRAVR"
BASE COUNT          971 a         2356 c         2476 g          867 t
ORIGIN      
        1 gccgaccagg gctacggaga ggccgcgtat cagctcggcg gcatgtacga gcgcggtatc
       61 ggggtcgccg cagacctcgc cgccgccggc ggctggtatc gacaggccgc caccgcgggc
      121 agcgtgccgg cggccttccg gctcgggcgg atgttcgccg ccggcgaagg ggtggaggcc
      181 gacttcggcc ttgcccgcga ctggtacctc caggccgcga cgcagggcca tgccggcgcc
      241 cagaacgcgc tcggcgagct ctacgacgac ggcctcggcg gcgaggagga cgtggccgag
      301 gcgctgcgct ggtatcgggc cgccgccgag cagggcgata ccgacgcgcg cgccaacatc
      361 gccctgctgc actacctcgg ccgcggcgtc gagcaggact acgccgccgc cttccgcggc
      421 tttcgcgaga ccgcgaacgc cggccacgtc gccgcccaat acaacctcgg agtcatgttc
      481 gagctcggcg agggggtgcc ggcgaacctt gagcaggcgg tgcagtggta cctgcgcgcg
      541 gccgagggcg gcgatgccga cgcccagacc aggctcggcg acatggccct cgccgggcgc
      601 ggcgtgcctc aggaccagct cgctgcgcgc aactggttcg aggacgcggc gggccagggc
      661 catgccgagg cgcaggtgaa gctcgccacg ttgctctatg acgggatcaa cggcatcacc
      721 gacatgggcc gcgccctgtt ctggttccgc gaggcggcgc gcgccggcaa cgccgaggcg
      781 cagtacaggc tggggatgat ggccgagcgt ggcctgggca tggcgccgga gcccgcgcag
      841 gcggcggaat ggtacgcact cgccgccgag gcggggcacg ccgacgccca gttcggccta
      901 gcgctgatgt acgagctcgg cgcgggcgtg ccggcgaacg atcaggccgc ggccagctgg
      961 tacgccaagg cggcggcgct cggccatgcc ggcgcccagg ccaggctcgg cgcgctctac
     1021 cggtccgggc gcggccttgc ccgcgacgtc gaccaggcgc gcggctggct cacccgtgcc
     1081 gccgagcagg gcgaggcgag cgcgctgtac aatctcggcc tgctcgacgc caacggcgag
     1141 ggcggcccgc gcaatccggt gcgcgggctg atgtggctgt cgctcgccgc cgacaagggc
     1201 cacgaggacg ccgcccgcga ccaggcgcgc ctcgccgcca gcatgtccga gcaggaggtc
     1261 gacaccgccc gccggctggc cgggatgtgg cgcgaccagc actggcgctg agcgcccgcc
     1321 ggagcgcacc ctacatggcg ctgccgccgg ccgcgcgcgg gcccgcccgc cgccgccccg
     1381 gccgggcgcc ctgggcgggg cgaggcgcga cgatgtcggg gacggtggcc tcgatgcggt
     1441 cgagggcaac cgtcttcagc gacgcgcggt gatggacata ctcgaacggg aacctgcggg
     1501 tggcgtcgaa gccgaccttg gcgccgaggt agtcggggaa gctcgggttg agcccgtggc
     1561 cgaaggtccg gtcgatgacc acgatgccgt gcttcgggtc gaggcgcgtc gccatcgccc
     1621 actcgacgtc ggtggcattg cggatgtcga cgtcgtcgtc gaccacggtc accatcttga
     1681 gcggcgggaa cgcggcgaag gcggcgagga tcgcctgctt cgaccagccg gcgcgcttct
     1741 gggcgatctg caccacggcg tggtagaagc cgcagccgcc gtggctgaaa tagacgtcgc
     1801 gcacgccggg cacctggcgc tggagcaaat tgagcacgtt cgcctcgccc agcagcccga
     1861 ccgagttgaa cacctcgcgg cccgagatga tggtctggaa taccggctcg cggcggtgat
     1921 ggagcgcgtg cacgtggacc accggccggg ggctgcgcag ggcgtagtag ccggtgacct
     1981 ccgcgaacgg cccctcgtcg gcgagctcgc ccggcgtgat ctcgcactcg agcgcaatca
     2041 ttgcatgggc aaccatctcc accggcgagc gcgtgcccgc caccaactcg agcggcgcgc
     2101 cgtggaactc gctggcgatg ccgagctcgt cggtctccgc cggcgccgcc tcggccgggg
     2161 tggcggcggc gaagtgcagg ccgggcccga cgccgacatt gaacgtgaac ggcaggcttt
     2221 cgccgcgcgc ggcggccttg tccaggtaca gctcgagatg ccgcccggcg tcgatgttga
     2281 cggtcatggt gttgcggccg gtgaccatga agcgctggat cgaggcgttg cgcacccccg
     2341 tctccgggtc gcgcgcgatc accacgccgg cgtcgaaata gggcccgccg tcgtcgagcg
     2401 catgggtcgg cacgggcagc cgggcgaggt cgacgcgcgt ctcggtcacc tccagcaccg
     2461 ggccgtccgc gaccaccacc ggcgggcgcg ggcgcctctg ccagcgcgcg atgcagccgg
     2521 cgacgtattg cggcagggtg cgctcgtcgc ggcgcatcag cgtcgccagc agctcgcgcg
     2581 accagtacag gccggtgaag acgggcatga cgtgcccctt gacctgctcg aacagcaccg
     2641 cgcgcgggcc gccctcgtgc gcggccgcga tcgcggcgag gtcgtggacc gggtcgacct
     2701 cggagtggac gcggatcagg cggccctcgg catcaagctg gtcgatgatc cgggcgaggt
     2761 cgctcaagta gttggcggcc atcggtttcc tccccatgcg cgcggtccgg cgccatggta
     2821 ggagcggccg ggagcgcttg ccaagtcggg agcgcggttg aacctgcccg cgatcgcacc
     2881 aaagtgtgtt gcacgcctgc acggggcgtg gtcttgtttc ctcaaggacg tgcacgtaac
     2941 gggagcacgc gatgattttc accggcctca accatctggc gttcatcacc aaggacctgg
     3001 agaagaccat ccgcttctac cgcgacctgt tggagatgga cctgttcgcc ggcatcggtc
     3061 acgacggctt tcgccactac ttcttcaagt gcggcgaggg ctcgatcgcc tttttcgagt
     3121 acgacatcgc caagccgatg gagtacgaca agttccacgg cacgccgacc gacaggccga
     3181 tcggcttcga ccacgtcgcg ctgaccgtgc cctcgcgcca ggacctgttc gcgctcaagg
     3241 accgtctcga gggcgctggc atcgaggtca ccggcccggt cgaccacggc gtgatgtggt
     3301 cgatctactt cttcgacccg accaacaacc tgccgctcga ggcgagctgg gactgcatgg
     3361 agctgctgca agcgccggcg atcgaggacg acgagccgat ggcgatcgcg gcggaaggcg
     3421 cgggcccgca gcccggccat tggccgagcc cgacccgcgc caccccgccc gacgagatgc
     3481 gcgcgcattc ggggaacggc tatgcgatgc gcgagagctt cctcaggcgc gggcttgcgc
     3541 gcaacaagcc cgatctcgac atcgcgctgg ccgaaggggc ggcaccggac aagcaggccg
     3601 ccgagtaact gccgccggca ccggccgcag aggcagccgt cagcccgccg gctcggcgtg
     3661 cttgccggcc gaccaggcgt cggcgagctt gcgcgcctgg tcgacggtga cggcctggta
     3721 ggcggcctgg gcgcgcaccc gctccgcatg cgacgagcgg gcctgcaggg agttccggcc
     3781 gcggctcatg cggtagaggc tctcgaagca ggcgtggtcg gcgctggcca gcttgcagac
     3841 cacggcgagc gtgcgcgggc cgtcgtcggc gagcgcgcgc tcgaccagcc gcggatcgac
     3901 accggcgagc aacgccaggc cctcgacgaa cacgtcgacg ctgccgcgaa acagcgcccc
     3961 ttgcaggaac ctgaggtcga gcgagccctt ggccgccagc gtctcgacga tcaggcgcgc
     4021 ccgcgacggc cggcgcaggt cggccgccgg tgcgctggcg gcggtgtcga ccgccgccgc
     4081 gatctcggca tcgagcaccg cggggtcgat gtggtaacgc tggcagatgc gctcgcggag
     4141 cggcgcggcc acccaggcac agagcttgcg cgccaggctc tcgggcaggt ccttgcggcg
     4201 cgcgagcgcc agctgatagg tctcgttggc tcgcgagtcc tcgaccagct cgcgcagcat
     4261 cgactcggtc agctgggcgc cggtgttctc gatcaccgcc gtgaccacgc ggtcgttgtc
     4321 ggtctcgatc agggcgccgc tcaccggcgt gctcagcgac cgtcgctggg caatcgcgag
     4381 ctggtgctcc tcggtgcgct cgcgaatgat ctcgatcagc gtctggtcgg acaacaccgg
     4441 gctctggcgc agcacgatct cggcgacctc gatcggctcg ctggcaaggt cggcgatcaa
     4501 tgcctcgggc atgtcgttgc ggctggcgag gcgacaggcg agctcgaggc gcagcttgac
     4561 ttcgacctcg cccagcaggt tacgcaggat gtcgctggca aggttgcgct cgcggtcgct
     4621 gtgcaaccag tcgtcttcga ccaggaattc ggagatggtt tggaagaccg ccgtgcgccc
     4681 ctgctcggag cgggtagcgg cgagccggac cagtgcctgg aagtcgatcg aggacgggtc
     4741 ggcgaggaca agctgctcgg tcatggtcgg cctttcgccg cattcgtgcg gctactgttc
     4801 ccttctggta ccggctccct cgccggacgg ccggcggagg aagcgcctgc gaggatcaag
     4861 ataagacggg ggcggccgat gtctcgcccg ccgagatgcc atcgcaacga agtgtgagcg
     4921 ccgtaacacg tggaaaatca gagagttcgc aaggggaaac ccccaaatga ctcgaacatc
     4981 gcgccgggtt cggactggcg caccaaagtg agcccatcga cggcgagcgg cgtaccgtcg
     5041 tagggcaggc aatcggcgct cagcgcggcc cgaatgcgct ccgccagccg ccggtcgagg
     5101 cgcccggtca gcgtcatgtg gaagcggaac cggtcgcgca catacggata gccccaccgg
     5161 cgcaagtggc ggcgctcgac cgcggacagg tcagccggcc cccgcttgcg ccattcggcc
     5221 gaagtcagcg gcgcccgaaa ccggtcgagg gccgtcaggc agctgtcggc caggcggtcg
     5281 agcgcggggc aaggcacgct cggcaccagg gcgacgaagc cgccgatcag cgccggcctg
     5341 agcggcgggc cggcgaggcg cggcgtcgcg gcggcgagcc gccgggtggc gcgcgccagg
     5401 gcgctcggct cgcacgcggc acgcaaccgg aacggtgcct tgagggtggc atggaagccg
     5461 tatcggcgcg gcgccccggt gattgccgcg agcacgcttt cggcgagccc cagccggtcg
     5521 gccatcgccc ggcgctgcgc ggtggcgtgc ggggcgccga accacgcctt gccgaaggcg
     5581 gcgagggcgg tttcggcggg cggcacgaaa taaaccgcga accgctccgg ggcgcagctc
     5641 atggcgccga cggcgcgccg gcgacggtgt cgagctcggc cagcagccgc gccgcctcgc
     5701 ggcggaccag ctcgcgctgg gcgtccttcg gcgggctcgc cgtcgcctgt gcgagcagcg
     5761 ctcgcgcgcg cgcggtgtcg ccgtggagcg cgaggagttg cttcgcgtat tccgtcggga
     5821 tcaacgccga cgccggcgcc agcgcgagcg cgcgctcgaa atgccgggtc gcctcgtcga
     5881 ggtcggcgcc gtagaacgcg ccggcgagga acgcgccgcc gcggcgcacc agctcgccgt
     5941 gccaggcgcc ggccaccgcg tgatagtagg cgttgtcgtc cgccttgcgc gcgaggccgt
     6001 ccagcaaccg gcccgattca tccgccagcc cggccaggaa cgcctggaag gcgtccatcg
     6061 cgcgcgcctg ctcgcccagg ctcaccacca gctgcaaggc ggcctcgaca tgtccccgat
     6121 cggcggcgag cgcacgcctg gcgaagcgtt cggcgtcgga cagcagcgcg cggcgctgct
     6181 cgccgccagc gagatagatc gcccgcacat tgctcgcccg cgccgccagc gccagcccgt
     6241 ccgcagtgcc gagcgccgcg ccggcgtcgg ctgcgtccgc gaaggcgccg tcgcggtaca
     6301 gcgactcggc ccgtgcgagc gcatcctcgg cggcaacagc ggccgccggc gcgcctgcga
     6361 ccagcgccag cgcgagcgcc acggccaggc gcagggcggt caccggacgg cgcggtcgcg
     6421 gcgccattcg aaggcgtcgt cgaacaggcg cgggtcgtcg aaatgacgcc ggccgacgaa
     6481 ggccgtggca tgggtgccga actggcgcat cgcgccggcg ctggcgatcc ccattggcca
     6541 cagcaggaag cgctcgagcc gctcgctgcc cgcgaccagc ccgtcgggcc gatagagcga
     6601 gcgccgcccg ccgcccgggg cggccaggct gcgcagctcc tcggccggga ccaggcgata
     6661 gccggccgcc
//
LOCUS       DASWHU010000094         6582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2258545, whole genome shotgun sequence.
ACCESSION   DASWHU010000094 DASWHU010000000
VERSION     DASWHU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258545"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..918
                     /gene="mdh"
                     /locus_tag="VGB88_03335"
                     /pseudo
     CDS             1..918
                     /gene="mdh"
                     /locus_tag="VGB88_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388965.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
     gene            1137..2306
                     /gene="sucC"
                     /locus_tag="VGB88_03340"
     CDS             1137..2306
                     /gene="sucC"
                     /locus_tag="VGB88_03340"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX9646504.1"
                     /translation="MNIHEYQAKALLARFHVAVPRGQVAYTAHEAADAARALGGTLWA
                     VKAQIHAGGRGKAGGIKVARSIEEVAKAAGAMIGSRLVTRQGGPEGKEVKRVYVEQGC
                     DIARELYLGLLLDRATARVTVMASAEGGVDIEEVAAASPEKILRVAIDPASGMQPFHA
                     RRIAFGLGLEDGQVASAVKFMLAIYTAYVETDASLIEINPLVVTGAGEVVALDAKMNF
                     DDNALYRHGDIADLRDESEEDPRELEAQRHDLSYIKLDGNIGCMVNGAGLAMATMDII
                     KLSGGAPANFLDVGGGASGERVSEAFKLILSDPDVEGVLVNIFGGIMRCDVIAEGIVE
                     AARMVSLHVPLVVRLEGTNVDLGKKILADSGLAIVAAADLADAAEKIVAAVREAA"
     gene            2306..3181
                     /gene="sucD"
                     /locus_tag="VGB88_03345"
     CDS             2306..3181
                     /gene="sucD"
                     /locus_tag="VGB88_03345"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9646505.1"
                     /translation="MAVLVDRDTRVICQGFTGRQGSFHSEQAIAYGTRMVGGVTPGKG
                     GTRHLDLPVFDTVHDAVAATGADASVIYVPPPFAADAMLEAVDAGVALVVCITEGIPV
                     ADMIRVKRVLAGSETRLIGPNCPGVITPGQCKIGIMPGHIHKPGRIGIVSRSGTLTYE
                     AVAQTTAAGLGQSSCIGIGGDPVNGTSFTDCLELFLADPETEGIIMIGEIGGSAEEEA
                     ADFLRASNRTKPVVGFIAGRTAPPGRRMGHAGAIIAGGVGGAAEKMAALRAAGATVAD
                     SPASLGTTMLSVLQG"
     gene            3241..6030
                     /locus_tag="VGB88_03350"
     CDS             3241..6030
                     /locus_tag="VGB88_03350"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX9646506.1"
                     /translation="MALGLDDLSFLTGANIGFIEELYERYRADPAALDPSWRDFFGEL
                     DRAPAALAAGAGRAAGAIAEDDLRGLIRDSLRMLMMIRAYRVRGHLIANLDPLGLEGR
                     KHHAELDPASYGFTDADYDRQFFIDGVLGHETASLRQILEILKQVYCSKTGIEFMHIQ
                     HPQEKAWIQQRVENTLNRTAYTPTERLEILRQLDIAEGFERFLHLKYPATKRFSIEGA
                     ESLVPALEAIIRCSVGHGVDEIVLGMPHRGRINVLTNILGKSYRLVFAEFQGESTHPG
                     DVLGSGDVKYHLGTVADRELADGRRVHLSLTANPSHLEWVDPVVVGRVRAKQTMRGDT
                     ARKRVMSVLMHGDAAFAGQGIVAETLGMSELRGHRTGGTVHVIVNNQIGFTTSPGYAR
                     SSPYPSDVAKTVQAPIIHVNGDDPEAVVHGATLAADFRHEFGRDVVLDIFCYRRHGHN
                     ETDEPAFTQPLMYKRIGELATARQLYTERLVAEGVLTPKDAEHRLETFQGMLADAYER
                     ARSFKPQKADWLQGQWAGLEQAPVGDRRGTTAVALETLREVGAGLCRVPEGFNAHRKA
                     LRFLDQRRQAIESGSDIDWATAEALAFASLLVEGHPVRLSGQDSGRGTFSQRHSVLVD
                     QKTEDRYVPLDHLGDGQARFEVTDSMLSEAAVLGFEYGYSLADPNALVLWEAQFGDFA
                     NSAQAIIDQFICAGESKWLRMSGLVLLLPHGYEGQGPEHSSGRLERFLQLYGQDNIQV
                     ANCTTPANYFHVLRRQLNRSFRKPLVVMTPKSLLRHKRCVSPLDDMGPGSSFHRVLWD
                     PARLVARTKVKRVVLCTGKVFYDLMEARAERAIKDVALVRIEQLAPFPDGPVAEALAM
                     YPKAEVVWCQEEPRNMGAWTFVAPRIEEVLAGLKIAGVRPRYVGRPEAASPATGLIKT
                     HLAEQAALLDQALTA"
     gene            6190..>6582
                     /locus_tag="VGB88_03355"
     CDS             6190..>6582
                     /locus_tag="VGB88_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9646507.1"
                     /translation="MTTDIIVPQLGESVTEATVAKWFKAAGDAVAADEILVELETDKA
                     NLEVTAEAAGVLAEILVGENESVDVGAVLGRIEEGAAAAARPGNGAAKAEPEPEAPAP
                     AAAPAPPSAPPAALSPAVRRLAAESGIDA"
BASE COUNT          993 a         2340 c         2341 g          908 t
ORIGIN      
        1 aatatcggcg gcactctcgc ccacctggtc gggctgggcg agctcggcga cgtgctgatg
       61 ttcgacatcg tcgacgggct ggcggagggc aagagcctgg acatcgcgca gtcctcgccg
      121 atcagcggct tcgacgcggc gttcaagggc gcctcgagct acgtcgcgat ccgcgacgcc
      181 gatgtggtga tcgtcactgc gggggtggcg cgcaagccgg gcatgagccg cgacgacctg
      241 ctcgacatca actgccgggt gatgcaggcg gtcggcgccg gcatcaagac ctattgcccg
      301 gacgcgttcg tcatctgcat caccaacccg ctcgacgcca tggtctgggt gctgcgcgag
      361 gcctcgggcc tggcgcatca ccgggtggtg ggcatggccg gggtgctcga ttcggcgcgc
      421 ttccggtact tcctcgccga ggagctcgac gtctcggtcg aggacgtgag cgcgttcgtg
      481 cttggcggcc acggcgacag catggtgccg ctggcgcgct actcgacggt cgcgggaatc
      541 ccgctgccgg acctggtcaa gatgggctgg accaccgccg agcggatcgg ggagatcatc
      601 cagcgggtgc gcgacggcgg cgccgagatc gttggcttgc taaagaccgg ctcggcgtat
      661 tatgcgccgg ccgcggcggc gatccagatg gccgaatcct atctcaagga caagaagcgc
      721 gtgctgccct gcgccgccta tctgaccggc gagtacgggg tcgacgggct ctatgtcggg
      781 gtgccggcgg tgatcggcgc caagggtgtg gagcgggtgg tcgagatcgc gctcaaccgc
      841 accgagaagg cccgcttcgc ccgctcggtc aaggcggtca aggagctggt cgacatctcg
      901 cgccggatgc tgcgccgcca ggccgcggcc aaggcgcgcg cggccaaggc gaagtcgccg
      961 gcccccgcgc gcggcggccg tcgcgggccc cagcgcgggt cgaggcgggc gggcgggcgc
     1021 acgcgccggc gcgtgcgttg agccggccgc gctcacggcg ggggatcgag ccggatgacc
     1081 ggcgccggcc gcgtcctgcc gcccggcgcg cctgatcgcg gcacgggcgc gcggcgatga
     1141 acatccacga gtaccaggcc aaggcgctgc tcgcccgctt tcacgtcgcg gtgccgcgcg
     1201 ggcaggtcgc ctataccgcg catgaggcgg cggacgccgc gcgcgcgctc ggcggcacgc
     1261 tgtgggcggt gaaggcccag atccatgccg gcgggcgcgg caaggccggc ggcatcaagg
     1321 tggcgcgctc gatcgaggag gtcgccaagg ccgccggcgc catgatcggc tccaggctgg
     1381 tcacccgtca gggcggcccc gagggcaagg aggtcaagcg ggtctatgtc gagcagggct
     1441 gcgacatcgc gcgcgagctt tatctcggcc tcttgctcga ccgcgccacc gcccgggtga
     1501 cggtgatggc gtccgccgag ggcggcgtcg acatcgaaga ggtggcggcc gcgagcccgg
     1561 agaagatcct gcgcgtcgcc atcgacccgg cctccggcat gcagccgttc catgcccgcc
     1621 gcatcgcctt cgggctcggg ctcgaggacg gccaggtggc ctcggcggtc aagttcatgc
     1681 tcgccatcta caccgcctat gtcgagaccg acgccagcct gatcgagatc aacccgctgg
     1741 tggtgaccgg cgccggcgag gtggtggcgc tcgacgccaa gatgaacttc gacgacaacg
     1801 cgctctaccg ccacggcgac atcgccgatc tgcgcgacga gagcgaggag gacccgcgcg
     1861 agctcgaggc gcagcgccac gacctcagct acatcaagct cgacggcaac atcggctgca
     1921 tggtcaacgg cgcgggcctg gcgatggcga ccatggacat catcaagctg tccggcggcg
     1981 cgccggcgaa cttcctcgac gtcggcggcg gcgccagcgg cgagcgggtc agcgaggcgt
     2041 tcaagctgat cctgtccgac cccgacgtcg agggcgtgct ggtcaacatc ttcggcggca
     2101 tcatgcgctg cgacgtgatc gccgaaggca tcgtcgaggc cgcgcgcatg gtcagcctgc
     2161 acgtgccgct ggtggtccgg ctcgagggca ccaatgtcga cctcggcaag aagatcctgg
     2221 ccgattcggg gctcgcgatc gtcgccgccg ccgacctcgc cgacgccgcc gagaagatcg
     2281 tggccgcggt cagggaggcc gcctgatggc cgtgctggtc gaccgcgaca cgcgggtcat
     2341 ctgccagggc ttcaccggcc gccagggctc gttccattcc gagcaggcga tcgcctatgg
     2401 cacgcgcatg gtcggcggcg tcacccccgg caagggcggc acccggcacc tcgacctgcc
     2461 ggtgttcgac accgtccacg acgcggtcgc ggcaaccggc gccgacgcct cggtgatcta
     2521 cgtgccgccg ccattcgccg ccgatgcgat gctggaggcg gtcgacgccg gggtggcgct
     2581 ggtggtgtgc atcaccgaag gcatcccggt cgccgacatg atccgggtca agcgcgtgct
     2641 cgccggctcc gagacccgcc tgatcgggcc caactgcccc ggcgtgatca cccccggcca
     2701 gtgcaagatc ggcatcatgc cgggccacat ccacaagccc gggcggatcg gcatcgtctc
     2761 gcgctcgggc acgctcacct acgaggcggt cgcccagacc accgccgccg gcctcggcca
     2821 gtcgagctgc atcggcatcg gcggcgaccc ggtcaacggc accagcttca ccgactgcct
     2881 ggagctgttc ctggccgacc ccgagaccga gggcatcatc atgatcggcg agatcggcgg
     2941 cagcgccgag gaggaggcgg ccgacttcct gcgggcatcg aaccgcacca agccggtggt
     3001 agggttcatc gccggccgca ccgcgccgcc cggcaggcgc atgggccatg ccggcgcgat
     3061 catcgccggc ggcgtcggcg gcgcggcgga gaaaatggcg gcgctgcgcg cggccggggc
     3121 cacggtcgcg gactcgccgg caagcctcgg caccaccatg ttgagcgtat tgcaggggta
     3181 gccaaggacc gccggagagg ttggcgcgcg tccccggcgg cgccttgggg aagacggaaa
     3241 atggcgctag gactcgacga cctgtcgttc ctcaccgggg ccaatatcgg tttcatcgag
     3301 gagctgtacg agcgttaccg ggcggacccg gcggcgctcg acccgagctg gcgcgacttc
     3361 ttcggcgagc tcgaccgcgc gccggcggcg cttgccgcgg gtgccgggcg cgcggcgggc
     3421 gcgattgccg aggacgacct gcgcggcctg atccgcgatt ccttgcgcat gctgatgatg
     3481 atccgcgcct atcgcgtgcg cggccacctg atcgccaacc tcgatccgct cgggctcgag
     3541 ggccgcaagc accacgccga gctcgatccc gcgtcctacg gcttcaccga cgccgactac
     3601 gaccgccagt tcttcatcga cggcgtgctc ggccacgaga ccgcgagcct gcgccagatc
     3661 ctggagatcc tgaagcaggt ctactgctcg aagaccggca tcgagttcat gcatatccag
     3721 cacccgcagg agaaggcgtg gattcagcag cgggtcgaga acacgctcaa ccgcaccgcg
     3781 tacaccccca ccgagcggct cgagatcctg cgccagctgg acatcgccga gggcttcgag
     3841 cgcttcctgc acctcaaata tccggcgacc aagcggttct cgatcgaggg cgcggagagc
     3901 ctggtgccgg cgctggaggc gatcatccgc tgctcggtcg gccacggcgt cgatgagatc
     3961 gtgctcggca tgccgcaccg cggccggatc aacgtgctga ccaacatcct gggcaagtcc
     4021 tatcggctgg tgttcgccga gttccagggc gagtccacgc atcctgggga cgtgctcggc
     4081 tcgggcgacg tcaagtacca tctcggcacc gtcgccgacc gcgagctcgc cgacggccgc
     4141 cgggtgcacc tgtcgctgac cgccaacccc tcgcacctgg aatgggtcga cccggtggtg
     4201 gtcgggcggg tgcgcgccaa gcagaccatg cgcggcgata ccgcgcgcaa gcgggtgatg
     4261 agcgtactga tgcacggcga cgccgcgttc gccggccaag gcatcgtcgc cgagaccttg
     4321 ggcatgtcgg agctgcgcgg ccaccgcacc ggcggcaccg tgcacgtcat cgtcaacaat
     4381 cagatcgggt tcaccaccag ccccggctac gcccgctcct cgccctatcc ctcggacgtc
     4441 gccaagaccg tccaggcgcc gatcatccac gtcaacggcg acgaccccga ggcggtggtc
     4501 cacggcgcga ccctggccgc cgacttccgc cacgagttcg gccgcgacgt ggtgctcgac
     4561 atcttctgct accgccgcca cggccacaac gagaccgacg agccggcgtt cacccagccg
     4621 ctgatgtaca agcgcatcgg cgagctcgcg accgcgcgcc agctctatac cgagcgcctg
     4681 gtcgccgagg gcgtgctcac gcccaaggac gccgagcacc ggctcgagac cttccagggc
     4741 atgctcgccg acgcctacga gcgcgcgcgc tcgttcaagc cgcagaaggc cgactggctg
     4801 cagggccaat gggccgggct cgagcaggcg ccggtcggcg accggcgcgg caccaccgcg
     4861 gtcgcgctcg agaccttgcg cgaggtcggc gccggcctgt gccgcgtgcc cgagggattc
     4921 aacgcccacc gcaaggcgct gcgcttcctc gaccagcgcc gccaggcgat cgagtccggc
     4981 agcgacatcg actgggcgac cgccgaggcg ctggcgttcg ccagcctgct ggtcgagggc
     5041 catccggtgc ggctcagcgg ccaggactcg ggccgcggca ccttctcgca gcgccactcg
     5101 gtgctggtcg accagaagac cgaggaccgc tacgtgccgc tcgaccacct cggcgacggc
     5161 caggccaggt tcgaggtcac cgacagcatg ctgtcggaag ccgcggtgct cggcttcgag
     5221 tacggctaca gccttgccga ccccaacgcg ctggtgctgt gggaggccca gttcggcgac
     5281 ttcgccaaca gcgcccaggc gatcatcgac cagttcatct gcgccggcga gagcaagtgg
     5341 ctgcgcatgt cgggcctggt gctgctgctg ccgcacggct acgagggcca gggccccgag
     5401 cacagctcgg gccggctcga gcgctttctc cagctttacg gccaggacaa catccaggtg
     5461 gcgaactgca ccacgccggc caactacttc cacgtcctgc gccgccagct caaccgcagc
     5521 ttccgcaagc cgctggtggt gatgacgccg aagtcgctgc tcaggcacaa gcgttgcgtc
     5581 tcgccgctcg acgacatggg gccgggctcg agcttccacc gggtgctgtg ggaccccgcg
     5641 cgcctggtcg cgcgcaccaa ggtcaagcgc gtcgtgctgt gcaccggcaa ggtgttctac
     5701 gacctcatgg aggcgcgcgc ggagcgcgcc atcaaggacg tggccctggt gcggatcgag
     5761 cagctggcgc cgttcccgga cggcccggtg gccgaggcgc tggccatgta cccgaaggcc
     5821 gaggtggtgt ggtgccagga ggagccccgc aacatgggcg cctggacgtt cgtcgcgccg
     5881 cgcatcgagg aggtgctggc cgggctcaag atcgccggcg tgcggccgcg atatgtcggc
     5941 cggcccgagg cggcctcgcc cgccaccggg ctgatcaaga ctcatttggc cgagcaggcg
     6001 gcactgctcg accaggcgct cacggcctag ccatgtcgct accggcccgc cgcccccctc
     6061 acccttccct ctccccccgc gaatcgcggg gggagagggt tgcgcagggt tggcgcggcc
     6121 accgccgcgc cttacccgga gctgggtgag ggggggctcc ggggcacgca ttcaagacgg
     6181 gttgacgtca tgaccaccga catcatcgtc ccccagctcg gcgaatcagt gaccgaggcg
     6241 accgtcgcca agtggttcaa ggccgcgggc gacgcggtcg ccgcggacga gatcctggtc
     6301 gaactcgaga ccgacaaggc caacctcgag gtgaccgcgg aggccgcggg cgtgctggct
     6361 gagatcctgg tcggcgagaa cgaaagcgtc gacgtgggcg cggtgctcgg gcgcatcgag
     6421 gaaggcgcgg cggccgcggc caggcccggc aacggcgccg ccaaggccga gcccgagccc
     6481 gaggcgccag cgcccgccgc cgcgcccgcg ccgccgtcgg cgcctcctgc cgcgctctcg
     6541 ccggcggtgc gccggctcgc ggccgagagc ggcatcgacg cc
//
LOCUS       DASWHU010000095         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3001084, whole genome shotgun sequence.
ACCESSION   DASWHU010000095 DASWHU010000000
VERSION     DASWHU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3001084"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VGB88_03360"
     CDS             complement(<1..1503)
                     /locus_tag="VGB88_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9646508.1"
                     /translation="MSAAARAAVCALLLPGAAIAQSPDFGELLAADPASWLQYGHDYG
                     AHRFVRLADIDRGNVSYLRPVWSVETGGDNPRLEATPLVHDGVVYMAAGDSRVLALDA
                     QTGARRWLFAPELPDAVGRVLCCGANNRGVALLDGLVFVGTVDARLIALDARTGVPAW
                     QAVVADWEQGYSITGAPLAVRDMVVTGVAGGEYGIRGFVAALDARTGALRWRTHVIPG
                     PGEAGHESWAGEAWRTGGGATWATGSYDAELDLVFWNTGNPAPWDCRLRPGDNKWTAG
                     TVALEAATGAIAWGFQYTPGDCWDRDSVSTPVLAELTINGRALKALLHYDKNGYFYVL
                     DRAHGRFVRGTAIVAGMNWADGLDPASGRPIVNPDMVPGAGGGEIGPVLTGNQGGVSW
                     QPLAWDPDRGVAYVLSNTLATSFDYLGADALAGAGPGDWYTAAVDEQYEVARVPQGGF
                     VAFDPARGRELWRRSDSLPFYAGALATAAGLVFTGDLHGRALALDADTGAL"
     gene            complement(1500..1907)
                     /locus_tag="VGB88_03365"
     CDS             complement(1500..1907)
                     /locus_tag="VGB88_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008958267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646509.1"
                     /translation="MIPFLAHFLFVLAGWTLVIKFAFPVAYALAEGAPAASYVLWDFW
                     WVVHLWLGWALLNWQRYSYLFAVTVSAVEIAIVTVKFALFLGAPEWTIWSTNWFVNKL
                     FVLACFALMLGYFTFARGRVRSYAQAGTAAAGG"
     gene            complement(1914..3122)
                     /locus_tag="VGB88_03370"
     CDS             complement(1914..3122)
                     /locus_tag="VGB88_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9646510.1"
                     /translation="MLDRRSFLGLGAAATVGLGGIDALAGRGEAGGVTPGIRSYVTLG
                     RTGLTISDISFGSSHSADPALVRHALTRGITYFDTAESYRFGTAEEAIGEGLRGVRDR
                     VVLATKTKAGAGDRRDDMMRALEGSLRRLGTDHVDIYFNHAVNDVARLANPEWREFTE
                     RAREQGKIRFRGVSGHGTRLVECLHYALDHDLADVVLCAYSFAQDPDFYDRLRHSLSY
                     VALQPELPKVLDKATAKGVGVIVMKTLMGGRLNDLREFETGGATFAQAAFRWVLASPR
                     VDALIVSMTSHAQIDEYVGGSGAAGLRGEDLRLLARYAGLNGAGYCQHGCNICAGACP
                     AGVEIAEVLRCRMYDVDYRDRDLARADYARLGTDATACLGCAHRACLGACPNGIPIAD
                     WTRDAARRLG"
     gene            3232..>3713
                     /locus_tag="VGB88_03375"
     CDS             3232..>3713
                     /locus_tag="VGB88_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646511.1"
                     /translation="MADKQGALIVGAGNGLSASLARLFAREGYAIALAARRIGKLDRL
                     CQETGAQAQACDAADPDQVAALFAWLDGALGVPAVVVYNPSFRTRGPLVELDAGDVAK
                     ALAITAFGGFLVSQQAARRMIEAGGGAILFTGASASVKGYAGSAPFAMGKFALRGLAQ
                     "
BASE COUNT          549 a         1314 c         1370 g          480 t
ORIGIN      
        1 cagcgcgccg gtgtccgcat cgagtgccag cgcgcgcccg tgcaggtcgc cggtgaacac
       61 cagcccggcc gcggtggcga gcgcaccggc gtagaacggg agcgaatcgc tccgccgcca
      121 tagctcgcgc ccgcgcgcgg gatcgaaggc gacgaacccg ccctgcggca cccgcgcgac
      181 ctcgtactgc tcgtcgaccg ccgccgtgta ccagtcaccg ggccctgcgc cggcgagcgc
      241 gtccgcgccg agatagtcga aagacgtggc gagcgtgttc gacaggacat aggcgacgcc
      301 gcggtcgggg tcccaggcga gcggctgcca cgagacgccg ccctggttgc cggtcagcac
      361 cggcccgatc tcgccgccgc ccgcgcccgg aaccatgtcc gggttgacga tcggccggcc
      421 gctcgcggga tcgaggccgt cggcccagtt catgccggcg acgatcgcgg tgccgcggac
      481 gaaccggccg tgagcgcgat cgaggacgta gaagtatccg ttcttgtcgt aatgcagcag
      541 cgccttgagc gcgcgcccat tgatggttag ctcggcaagc accggggtgc tgacgctgtc
      601 gcggtcccag cagtcgccgg gcgtgtactg gaagccccag gcgatcgccc cggtcgccgc
      661 ctcgagcgcg accgtgcccg cggtccactt gttgtcgccc ggacgcagcc ggcagtccca
      721 cggcgccgga ttgccggtgt tccagaacac gaggtcgagc tcggcatcgt aggacccggt
      781 cgcccaggtg gcgccgccgc cggtgcgcca ggcctcgccc gcccaggact cgtgcccggc
      841 ctcgccgggg ccggggatca cgtgggtgcg ccagcgcagg gcgccggtgc gcgcatcgag
      901 cgcggcgacg aagccgcgga tgccgtactc gccgccggcg acgcctgtca ccaccatgtc
      961 gcgcaccgcg agcggcgcgc cggtgatgct gtagccctgc tcccagtcgg cgaccacggc
     1021 ctgccacgcg ggcacgccgg ttcgggcgtc gagcgcgatc aggcgcgcgt ccaccgtgcc
     1081 gacgaacacc agcccgtcga gcagggcgac gccgcgattg ttggcgccgc agcacagcac
     1141 gcgcccgacc gcgtcgggaa gctccggcgc gaacagccag cggcgcgcgc cggtctgcgc
     1201 atcgagcgcg agcacgcgcg agtcgcctgc ggccatgtag acgacgccgt catgcacgag
     1261 cggcgtcgcc tcgaggcgag gattgtcgcc gccggtctcg accgaccaca ccgggcgcag
     1321 gtacgaaaca ttgccgcggt cgatgtcggc aaggcggacg aacctgtgcg caccgtagtc
     1381 gtggccgtac tggagccagg acgcggggtc ggcggcgagc agctctccga agtccggcga
     1441 ctgggcgatg gccgcgccgg gcagcaggag cgcgcacacg gccgcccggg cggcggcgct
     1501 cacccgccgg cggccgccgt ccccgcttgg gcgtaactgc gcactcggcc gcgggcgaag
     1561 gtgaaatagc ccagcatcag cgcgaagcag gcgagcacga acagcttgtt gacgaaccag
     1621 ttggtgctcc agatggtcca ctccggcgcg cccaggaaca gcgcgaactt gaccgttacg
     1681 atggcgatct cgaccgccga gacggtgact gcaaagagat atgaatagcg ttgccaattg
     1741 agcaacgccc agcccagcca caaatgaacg acccaccaga agtcccagag gacgtagctg
     1801 gccgccggcg cgccctcggc gagcgcgtag gcgaccggga aggcgaactt gatcaccagg
     1861 gtccagcccg ccagcacgaa caggaaatgg gcgaggaacg ggatcacgcg gcctcagccg
     1921 aggcggcggg cggcgtcgcg cgtccagtcc gcgatcggga tgccgttcgg gcaggcgccg
     1981 aggcaggcgc ggtgggcgca gccgagacac gccgtggcat cggtgccgag gcgggcgtag
     2041 tcggcgcgcg cgagatcgcg gtcgcggtag tcgacgtcgt acatgcggca gcgcagcacc
     2101 tcggcgatct cgaccccggc cgggcacgcg cccgcgcaga tgttgcagcc gtgctggcag
     2161 tagccggcgc cgttgaggcc ggcgtaacgg gcgagcaggc gcaggtcctc gccgcgcagg
     2221 cccgccgcgc ccgagccgcc gacatactcg tcgatctggg cgtggctggt catcgacacg
     2281 atcaaggcat cgacgcgcgg gctcgccagc acccagcgga acgcggcctg ggcgaaggtc
     2341 gcgccgccgg tctcgaactc gcgcagatcg ttgagccggc cgcccatcag ggtcttcatc
     2401 acgatcacgc cgacgccctt ggccgtggcc ttgtccagca ccttgggcag ctcgggctga
     2461 agcgcgacgt agctcaagct gtggcgcagc cggtcgtaga agtcgggatc ctgggcgaag
     2521 ctgtaggcgc acagcaccac gtcggcgagg tcgtggtcga gcgcataatg caggcactcg
     2581 accaggcggg tgccgtggcc ggagacgccg cggaagcgaa tcttgccctg ctcccgggcg
     2641 cgctcagtga actcccgcca ctccgggttc gcgagccggg cgacgtcgtt gacggcgtgg
     2701 ttgaagtaga tgtcgacgtg gtcggtgccg aggcgccgga ggctgccttc gagcgcgcgc
     2761 atcatgtcgt cccgccggtc gccggcgccg gccttggtct tggtcgcgag cacgaccctg
     2821 tcgcgcaccc cgcgcaggcc ctcgccgatc gcctcctcgg cggtgccgaa gcggtagctc
     2881 tcggcggtgt cgaaataggt gatgccgcgg gtgagcgcgt gacgtaccag cgcggggtcc
     2941 gcggagtggc tcgagccgaa cgagatgtcc gagatcgtga gcccggtgcg cccgagggtg
     3001 acgtagctgc ggatgcccgg cgtcacgccg ccggcctcgc cgcggccggc cagcgcatcg
     3061 atcccgccga gcccgacggt cgccgccgcg ccgaggccca ggaagctgcg tcggtcgagc
     3121 atctcggact cgcccctcga accgctttcg caaggccatg gtacggggcg cgccgcgctt
     3181 gttcaatgcc cgccggtcga gtctaatggc cgcggaaggt gacggcaggt catggcggac
     3241 aagcaaggcg cgctcatcgt cggcgcgggc aacggcctca gcgcctcgct ggcgcggctg
     3301 ttcgcgcgcg agggctacgc gatcgcgctc gccgcgcggc gcatcggtaa gctcgaccgc
     3361 ctgtgccagg agaccggtgc gcaggcgcag gcgtgcgatg ccgccgaccc cgaccaggtc
     3421 gcggcgctgt tcgcgtggct cgacggcgcg ctcggcgtgc ccgcggtggt ggtctacaac
     3481 ccgagcttcc gcacccgcgg gccgctggtc gagctcgatg ccggcgacgt cgccaaggcg
     3541 ctcgcgatca ccgcgttcgg cggcttcctg gtcagccagc aggcggcgcg gcggatgatc
     3601 gaggcgggcg gcggcgcgat cctgttcacc ggcgcctcgg cgagcgtcaa gggctatgcc
     3661 ggctcggcgc cgttcgcgat gggcaagttc gcgctgcgcg gcctcgctca gag
//
LOCUS       DASWHU010000096         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3002528, whole genome shotgun sequence.
ACCESSION   DASWHU010000096 DASWHU010000000
VERSION     DASWHU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3002528"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VGB88_03380"
     CDS             complement(<1..798)
                     /locus_tag="VGB88_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9646512.1"
                     /translation="MLTTRLLRTSSFQIALLYLTLFGATLLALLAFVYWSTAGLIDRQ
                     MSDTVEAEIRGLGEQYRDEGLSRLVEVIGERSGRNGDPSNVYLLADPGLKPLAGNLSR
                     WPEVAAGADRWLEVRLHRFGDEQGNPHTIRGRMIDLPGGLHLFVGRDTEERGDFRDIV
                     MGAMAWALIPALVLGLVGGVLIGRYSLRRVDMVRATGQEIINGDLSRRVPLSGSGDEF
                     DRLALTINEMLDQIETLMAGMRAVTDSLAHDLRSPLTRAKGSIEQALR"
     gene            complement(785..1462)
                     /locus_tag="VGB88_03385"
     CDS             complement(785..1462)
                     /locus_tag="VGB88_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9646513.1"
                     /translation="MRILVIEDDDKTADYIVKGLAECGYVADRAADGGDGLHLATGGH
                     YDALVVDRMLPGLDGLAVVRALRAGEISVPVLILSALAHVDERVTGLRAGGDDYLTKP
                     FAFSELQARLEALLRRPRALAQATVLEVGDLSVNLLARKVMRAGRPIELRPQEYKLLE
                     YLMRHAGEVVTRTMLFEGVWDFHFDPQTNVVDVHISRLRQKIDKGFERPLIHTHRGGG
                     YSISADD"
     gene            complement(1640..>2300)
                     /locus_tag="VGB88_03390"
     CDS             complement(1640..>2300)
                     /locus_tag="VGB88_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9646514.1"
                     /translation="HGKVERGWLGVMIQKVTDEMASALGLDGAAGAIVTEVKPDGPAA
                     AAGLRQGDVILAFDGTRVSDARTLARLVAARPAGTAAPITVWRDRSEHALSVVTGEQP
                     ARVAELEPAESQGQGGAWHSEALGADLAALTPERRAETGIAEDVAGVLVTDVKSGSVF
                     EQGLRPGDVIKEVARTPVTSPEQVEALVEKARSQAKGAVLMLVSRQGHDLFLGLKLGV
                     A"
BASE COUNT          310 a          845 c          812 g          333 t
ORIGIN      
        1 gcgcagcgcc tgctcgatgc tgcccttggc gcgggtcagc gggctgcgca ggtcgtgcgc
       61 caggctgtcg gtgaccgcgc gcatgccggc catcagggtc tcgatctggt cgagcatctc
      121 gttgatggtc agcgccaggc ggtcgaactc gtcgccgctg ccggacagcg gcacccgccg
      181 cgacaggtcg ccgttgatga tctcctggcc ggtggcgcgc accatgtcga cccggcgcag
      241 cgagtagcgg ccgatcagca cgccgccgac caggcccagc accagtgccg ggatcagcgc
      301 ccaggccatc gcgcccatca cgatgtcgcg gaagtcgccg cgctcctcgg tatcgcggcc
      361 gacgaacaga tgcagccctc cgggcaggtc gatcatgcgc ccgcggatgg tgtgcgggtt
      421 gccctgctcg tcgccgaagc ggtgcaggcg cacctcgagc cagcgatcgg cgccggccgc
      481 gacctcgggc cagcgggaca ggttgccggc cagcggcttg agcccgggat cggcgagcag
      541 atagacgttg gacggatcgc cgttgcggcc gctgcgctcg ccgatcacct cgaccaggcg
      601 gctcaggccc tcgtcgcggt actgctcgcc gaggcccctg atctcggcct cgaccgtgtc
      661 gctcatctgg cggtcgatca ggccggcggt ggaccagtag acgaacgcga gcagcgccag
      721 cagggtggcg ccgaacaggg tcaggtagag cagcgcgatc tggaagctcg aggtgcgcag
      781 cagcctagtc gtcagcactg atcgaatacc cgccgccgcg atgggtgtgg atcagcggcc
      841 gctcgaagcc cttgtcgatc ttctgcctga gccggctgat atggacgtcg acgacgttgg
      901 tctgcgggtc gaagtggaag tcccacacgc cctcgaacag catcgtgcgg gtgaccacct
      961 cgccggcatg gcgcatcagg tactcgagca gcttgtactc ctgcggcctg agctcgatcg
     1021 gccggccggc gcgcatcacc ttgcgcgcca gcaaattcac gctcaggtcg ccgacctcga
     1081 gcacggtcgc ttgggcgagc gcgcgcggcc ggcgcagcag cgcttccagc cgcgcctgaa
     1141 gctcggagaa cgcgaacggc ttggtcaggt agtcgtcgcc gccggcgcgc agcccggtga
     1201 cccgctcatc gacatgggcg agcgcgctca ggatcagcac cggcaccgag atctcgccgg
     1261 ccctgagcgc gcgcaccacc gccagcccgt cgaggccggg cagcatgcgg tcgacgacca
     1321 gcgcgtcgta gtggccgccg gtggcgaggt gcaggccgtc gccgccgtcc gcggcccgat
     1381 cggcgacgta gccgcattcg gcgaggccct tgacgatgta gtcggcggtc ttgtcgtcgt
     1441 cctcgatgac caggatgcgc atgccttcgc cgctggccag cctcggccag gtctcctcga
     1501 gccggtcgcc gccgttatat ggtgcgcgcg cggtcatcgc cgcaatcccg tgacgtttcg
     1561 ccggcggcgc aggaacggca ggcgggccgc ccgctgcgat tccggacggc ccgcccgtcg
     1621 gcgccatggc ctcgccgtgt caggcgacgc cgagcttgag gccgaggaac aggtcgtgcc
     1681 cctggcggct gaccagcatc agcacggcgc ccttggcctg cgacctggct ttctcgacca
     1741 gcgcctcgac ctgctcgggc gaggtgaccg gggtgcgggc gacctccttg atcacgtcgc
     1801 ccggcctcag gccctgctcg aacaccgagc ccgacttgac gtcggtcacc agcacgccgg
     1861 cgacgtcctc ggcgatgccg gtctcggcgc ggcgctcagg cgtcagcgcc gcgaggtccg
     1921 cgccgagcgc ctcggagtgc caggcgccac cctggccctg gctctcggcc ggctcgagct
     1981 ccgccacccg tgccggctgc tcgccggtga ccaccgacag ggcgtgctcg gaccggtcgc
     2041 gccagaccgt gatcggcgcc gcggtgccgg ccgggcgcgc cgcgaccagg cgcgccagcg
     2101 tgcgcgcatc gctcacccgc gtgccgtcga aggcgaggat cacgtcgccc tgcctgaggc
     2161 cggcggccgc ggccgggccg tcaggcttga cctcggtgac gatcgcgccc gcggcgccgt
     2221 cgaggccgag cgcgctcgcc atctcgtccg tcaccttctg gatcatgacc ccgagccagc
     2281 cgcgctcgac cttgccgtga
//
LOCUS       DASWHU010000097         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3363526, whole genome shotgun sequence.
ACCESSION   DASWHU010000097 DASWHU010000000
VERSION     DASWHU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3363526"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="eno"
                     /locus_tag="VGB88_03395"
     CDS             complement(<1..650)
                     /gene="eno"
                     /locus_tag="VGB88_03395"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423033.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9646515.1"
                     /translation="MSAITDIRAREILDSRGNPTVEVEVELETGVVGRAAVPSGASTG
                     KHEAVELRDRDAARYGGKGVLHAVESVNGEIFDALSGRDAGEQGVLDRLLVELDGTED
                     KRRLGANAILGASLAVAHAAADELELPLYAYIGGCYAHLLPVPMMNIVNGGVHADNSL
                     DLQEFMVVPHGAASFAEALRWGAEVFHALKRLLAADGHATAVGDEGGFAPNLASNDA"
     gene            complement(669..1541)
                     /gene="kdsA"
                     /locus_tag="VGB88_03400"
     CDS             complement(669..1541)
                     /gene="kdsA"
                     /locus_tag="VGB88_03400"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX9646516.1"
                     /translation="MPDDRTPPGRSRHVAVGGLTLGNDLPLVLIAGPCALESRAHALE
                     MSQALVELAVALGIGLIYKTSFDKANRTSLDSPRGLGLEASLPIFVELRETYGCPMLT
                     DVHTEEQCARAAEAVDVLQIPAFLCRQTDLLVAAARTGRAVNVKKGQFLAPWDMVNVV
                     RKIATSGNARILVTERGASFGYNTLVSDMRALPTLAETGCPVVFDATHSVQQPGGLGG
                     ASGGQREYVPVLARAAVAVGVAAVFMETHQDPDHAPSDGPNMVPLKHVRGLLDTLKAF
                     DSIAKQRPLASVAP"
BASE COUNT          220 a          576 c          589 g          235 t
ORIGIN      
        1 gcgtcgttgc tcgccaggtt gggcgcgaag ccgccctcgt cgccgaccgc ggtcgcgtgg
       61 ccgtcggcgg cgagcaggcg cttgagcgca tggaacacct cggctcccca gcgcagcgcc
      121 tcggcgaagc tggcggcgcc gtgcggcacg accatgaact cctgcaggtc gagcgagttg
      181 tcggcatgga cgccgccgtt gacgatgttc atcatcggca ccggcaacag gtgcgcgtaa
      241 cagccgccga tataggcgta gagcggcagc tcgagctcgt cggccgcggc gtgcgcgacg
      301 gcgaggctgg cgccgaggat ggcgttggcg ccgagccggc gcttgtcctc ggtgccgtcg
      361 agctcgacca gcagccggtc cagcaccccc tgctcgccgg cgtcgcgccc cgacaaggca
      421 tcgaagatct cgccgttgac cgactcgacc gcgtggagga cgcccttgcc gccgtaacgc
      481 gcggcatcgc ggtcgcgcag ctcgaccgcc tcgtgcttgc cggtcgaggc gccggacggc
      541 accgccgcgc gcccgaccac gccggtctcg agctcgacct cgacctcgac cgtggggttg
      601 ccgcggctgt ccagtatctc gcgggcgcgg atgtcggtga tcgctgacat tgcggtttgc
      661 aggccggctc agggcgcgac cgaggcgagc ggtcgttgct tggcgatcga atcgaacgcc
      721 ttgagcgtgt cgagcaggcc gcgcacgtgc ttcagcggca ccatgttcgg gccgtcgctc
      781 ggggcgtgat ccgggtcctg gtgggtctcc atgaacaccg ccgcgacgcc gaccgccacc
      841 gccgcccgtg ccagcaccgg cacgtactcg cgctggccgc cgctcgcccc gccgagtccg
      901 cccggctgct ggaccgagtg ggtggcgtcg aacaccaccg gacagccggt ctcggcgagc
      961 gtcggcagcg cgcgcatgtc cgagaccagc gtgttgtagc cgaagctcgc gccgcgctcg
     1021 gtgaccagga tgcgcgcgtt ccccgacgtg gcgatcttgc gcaccacgtt gaccatgtcc
     1081 cagggcgcga ggaactgccc cttcttgacg ttgaccgcgc gcccggtgcg cgccgccgcc
     1141 accagcaggt cggtctggcg gcacaggaac gccgggatct gcagcacgtc gaccgcttcg
     1201 gccgcgcgcg cgcactgctc ctcggtgtgc acgtcggtca gcatcgggca gccataggtc
     1261 tcgcgcagct cgacgaagat cggcaggctg gcctcgagcc ccagcccgcg cgggctgtcg
     1321 aggctggtgc ggttggcctt gtcgaacgag gtcttgtaga tcagcccgat gccgagggcc
     1381 acggccagct cgaccagcgc ctggctcatc tccagggcat gggcccggct ctccagcgcg
     1441 cacgggccgg caatcagcac cagcggcaga tcgttgccga gggtgaggcc gccgacggcg
     1501 acgtgccggc tgcggccagg aggcgtgcgg tcgtcgggca tgggcggcct acatcaggcg
     1561 cgactgctgc acggcggccg cgacgaacga ggtgaacagc gggtgcgggt cgaacggctt
//
LOCUS       DASWHU010000098         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1517023, whole genome shotgun sequence.
ACCESSION   DASWHU010000098 DASWHU010000000
VERSION     DASWHU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517023"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGB88_03405"
     CDS             <1..249
                     /locus_tag="VGB88_03405"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX9646517.1"
                     /translation="RRWLVCGGGRHNPALMAALAARLGAAVAPVEAVGWQGDALEAQA
                     FAYLAVRSLRALPLSLPSTTGVPAPTCGGVLVRARDVA"
     gene            complement(246..1505)
                     /gene="metZ"
                     /locus_tag="VGB88_03410"
     CDS             complement(246..1505)
                     /gene="metZ"
                     /locus_tag="VGB88_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX9646518.1"
                     /translation="MSASTSSVPPPSGPARARAGQAQAGARVWRRQTQCVRGGLERSP
                     HGETCEALYLTSGFRYDSAEAAEDRFSGEVPGFVYSRYANPTVAMFESRLALIEGAEA
                     CSATASGMAAVTAALMCQLEAGDRVVCARTMFGSCNYVLTELLPRFGIDVELVDGPDI
                     EGWRRALAGPARCVFFETPANPTLVLVDIAAVSALAHAAEARVVVDNALATPLLQSPL
                     ALGADVVVYSATKHIDGQGRCLGGAILGSESFRDDLLRPFLRHTGPALSPFNAWLLLK
                     GLETLELRLERQCRTAARLADLLAGHRAVRRLYYPGRADHPQAGLAARQMTGGGTLVS
                     FEVAGGRAGAFALMNALELIDISNNLGDSKSLITHPASSTHRSLTPIERQRIGVGDTL
                     LRLSVGLEDVHDLADDLARALDAALAA"
     gene            complement(1795..>2212)
                     /locus_tag="VGB88_03415"
     CDS             complement(1795..>2212)
                     /locus_tag="VGB88_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9646519.1"
                     /translation="LSDAASALDWLQSYNPDARACWIAGFSFGAWIALQLLMRRPEIN
                     GFIAVAPPARIYDFSFLAPCPVSGQVIQGSADDIVPVDHVRKLVTKLSSQRDVKVDFR
                     IVDGADHFFNGHLEQVASAVTGYIGERMRHIKVDEA"
BASE COUNT          306 a          790 c          833 g          283 t
ORIGIN      
        1 cggcgctggc tggtgtgcgg cggcgggcgc cacaacccgg cgctgatggc cgcgctcgcc
       61 gcgcgcttgg gcgcggcggt ggcgccggtc gaggcggtcg gctggcaggg cgacgcgctc
      121 gaggcgcagg ccttcgccta cctcgcggtg cgcagcctgc gcgcgctccc cttgagcctg
      181 ccctcgacca ccggcgtgcc cgcgccgacc tgcggcgggg tgctggtccg cgcccgcgac
      241 gtggcctagg cggccagggc ggcgtcaagc gcgcgcgcca ggtcgtcggc gaggtcgtgg
      301 acgtcctcga gcccgaccga cagcctgagc agggtgtcgc cgaccccgat ccgctggcgc
      361 tcgatcggcg tcagcgagcg gtgcgtgctg ctcgccgggt gggtgatcag gctcttggag
      421 tcgccgaggt tgttcgagat gtcgatcagc tcgagcgcgt tcatcagcgc gaacgcgccc
      481 gcgcgcccgc cggcgacctc gaagctcacc agggtgccgc cgccggtcat ctggcgcgcg
      541 gcgagcccgg cctgggggtg gtcggcgcgg cccgggtagt acagccggcg caccgcccga
      601 tgcccggcca gcaggtcggc gaggcgggcg gcggtgcggc actggcgctc aagcctgagc
      661 tcgagggtct cgaggccctt gagcagcagc caggcgttga acgggctcag cgccggcccg
      721 gtgtggcgca ggaacgggcg caacaggtcg tcgcggaacg actcgctgcc gaggatcgcc
      781 ccgcccaggc agcggccctg gccgtcgatg tgcttggtcg ccgagtagac gaccacgtcg
      841 gcgccgagcg cgagcgggct ctgcagcaac ggcgtcgcca gcgcgttgtc gaccaccacc
      901 cgcgcctccg cggcatgcgc gagcgcggac acggcggcga tgtcgaccag gacgagcgtc
      961 gggttggcag gggtctcgaa gaacacgcac cgcgcgggcc cggcgagcgc gcgccgccag
     1021 ccctcgatat cggggccgtc gaccagctcg acatcgatcc cgaaccgcgg cagcagctcg
     1081 gtcagcacat agttgcacga gccgaacatg gtgcgcgcgc acaccacccg gtcgccggcc
     1141 tcgagctggc acatcagcgc cgcggtcacc gccgccatgc cgctcgccgt cgcggagcac
     1201 gcctccgcgc cctcgatcag cgcgaggcgg gactcgaaca tggcgacggt cgggttggcg
     1261 tagcgcgagt agacgaagcc gggcacctcg ccggagaacc tgtcctcggc ggcctcggcg
     1321 ctgtcgtagc ggaagcccga ggtcaggtac agtgcctcgc aggtctcgcc gtggggcgag
     1381 cgctcgagcc cgccgcgcac gcactgggtc tggcgccgcc agacgcgcgc gcccgcctgc
     1441 gcctggccgg cgcgcgcgcg tgccggccca gaaggcggcg gaacggatga ggtggatgcg
     1501 gacatgggac ctctcctcca acgtggcggg cccacggaga gatccccgtg gcgctttagc
     1561 gttggtgacg cggcgcaagc tgcccgatca aatcccgcgg gtccgtcggg gtggaccagt
     1621 cccggatggc cgcaggcgcg acgccccact gtgcggctgc ggcggtgccg ccacgacgct
     1681 cagaagcccg tggggtgcgc acccccgagc cgtcgcccaa ttggcacacc gccgatgatg
     1741 cacgcgcgcc ccaggctgtc aagcgggccc cccgaatgat ttccggcgcg gctctcaggc
     1801 ctcgtcgacc ttgatgtggc gcatgcgctc gccgatgtag cccgtaaccg cgctcgccac
     1861 ctgctcgagg tggccgttga agaagtggtc ggcgccgtcg acgatgcgga aatcgacctt
     1921 gacgtcgcgc tggctcgaca gcttggtgac cagcttgcgc acgtggtcga ccggcacgat
     1981 gtcgtcggcc gagccctgga tcacctgccc cgagaccggg cagggggcga ggaacgagaa
     2041 gtcgtagatg cgcgccggcg gcgccacggc gatgaagccg ttgatctcgg gccggcgcat
     2101 cagcagctgg agcgcgatcc aggcgccgaa cgagaagccg gcaatccagc acgcgcgcgc
     2161 gtccgggttg taggactgca gccagtcgag cgccgaggcg gcatcgctca gc
//
LOCUS       DASWHU010000099        11029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1895523, whole genome shotgun sequence.
ACCESSION   DASWHU010000099 DASWHU010000000
VERSION     DASWHU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895523"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="trpS"
                     /locus_tag="VGB88_03420"
     CDS             <1..843
                     /gene="trpS"
                     /locus_tag="VGB88_03420"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9646520.1"
                     /translation="QLTANTREVTAGLVAAGIDPVRDIVFNQSQVPAHAELAWIFNCV
                     ARLGWLNRMTQFKEKAGKHRENATVGLFAYPNLMAADILLYKATHVPVGDDQKQHLEL
                     ARDIANAFNSMFGVDFFPAPEPMILDAAARVMSLRDGTKKMSKSDPSDYSRLNMTDSA
                     DQIALKIRKARTDPDPLPATPDGLASRPEAANLVGIYAALADRSVAEVCAEFAGAPFS
                     DFKARLTDLAVATLGPIGAEMKRLAADPGAIDSVLRDGAHRARALAAPVLAEAKRIVG
                     FLSP"
     gene            910..1938
                     /locus_tag="VGB88_03425"
     CDS             910..1938
                     /locus_tag="VGB88_03425"
                     /inference="COORDINATES: protein motif:HMM:NF021600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEX9646521.1"
                     /translation="MFDDDADLPDRTGTAATIPAEAPPAPAAEAGAGRPRGARRRAAR
                     TAAALLIAALVLYYPVGMVLTHRIDDDPDFTFAPETFPESGSRAVALAAALMEHQVDD
                     AGWIPNNPFFYASAALDNMPNFQQGLRASVFRFAIEMTDQIGRTRGSSQVDPDLDIAA
                     GEFKFPGDVWIWDPGVSLLPQATSEQRYRRAIRHLRAYNQRLGAGTATFDSRADNLLA
                     TLDRFASDLGSASAEIDQHIAAYTGLFDLTADDLFYNVKGRVYGYYMILTELGGDFEA
                     VLAERQLQAAWAQMLASMRTAATLQPWVVLNGAPDGQLVPNHLAAQGFWLLRGRTQLR
                     EITNILLK"
     gene            2049..2963
                     /locus_tag="VGB88_03430"
     CDS             2049..2963
                     /locus_tag="VGB88_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9646522.1"
                     /translation="MDIYLPVAEMSVNVLVLLGMGGVIGFLSGLFGVGGGFLLTPLLI
                     FIGVPPPVAVASQANQIAAASVSGLLAHWRRGNVDFRMGWVLLAGGALGSSVGVWLFR
                     VLREIGQIDAAISISYVVFLGSVGLLMAVESANTILRRRRGAPRRKLHQHIWLHRLPL
                     KLRFRRSKLYISAISPALIGLLVGVLAAVMGVGGGFVMVPAMIYLLGMPTSVVIGTSL
                     FQIMFVTAIVTVLHAATNQTVDMVLALILLAGAVIGAQFGSRYVAALRGEQLRGLLAL
                     MVLVVCAKLAFDLVVMPDDPYSLTPVVQ"
     gene            2960..3733
                     /locus_tag="VGB88_03435"
     CDS             2960..3733
                     /locus_tag="VGB88_03435"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX9646523.1"
                     /translation="MTGGAAVRHGVAGIAALAALAALVALAAGRAGADYLVADLSDHR
                     IAITSGFTGSEVLLFGTRSGRGDVVVVLRGPKAPSVVWRKLRTLGMWLNRVQVRFDDA
                     PGYYAVAASRPLDRLAPAKLLEDNEIGFANLRLAASRVDPRANLADFREALLRSMERA
                     RLYRAAISEVVFIDDILFRATFAFPANVPTGLYNAHVYLIDDGVLVSKRTAELEISKT
                     GFEAAMFRFANHQPLAYGLVAVLVAVVAGWSGGMMFRGR"
     gene            3730..4227
                     /locus_tag="VGB88_03440"
     CDS             3730..4227
                     /locus_tag="VGB88_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9646524.1"
                     /translation="MTAMAVAEQHVAGRVKFLVCVDDSKDCRVALRFASLRARNTEGY
                     LVLLYVIEPGDFQHWLAVGKLMEEEKRAEAEQILNALAAEVHEFAGIHPVLVVREGRK
                     ADEILRLIDEDPTINILVLGAAPEGEGSNELVRKLSGELTKRLSIPLTIVPGNLTDAQ
                     LAMLT"
     gene            4336..4902
                     /locus_tag="VGB88_03445"
     CDS             4336..4902
                     /locus_tag="VGB88_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9646525.1"
                     /translation="MFIQTEPTPNPATMKFLPGEPVLADGTANFTDAEAAGRSPLAGR
                     LFQIPGIEGVFFGSDFVTVTKSDAAEWQLLKPSILGVIMEHYVSGAPIMVAGAGGEAG
                     ARGPEAAEADAEIVAQIKELLDTRVRPAVAQDGGDIIFHGYESGIVLLEMHGSCSGCP
                     SSTATLKHGIENMLRHYIPEVVEVRAIM"
     gene            complement(4931..5995)
                     /locus_tag="VGB88_03450"
     CDS             complement(4931..5995)
                     /locus_tag="VGB88_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9646526.1"
                     /translation="MSAAGRRVGRSGLARRLGRGALGCALALAATGAAGWAQAQDSAV
                     ILTYQRFAAVGGPLTTTLAELDAHLAHLAGGGYAVLPLPRVVAALAGGAALPERTVAI
                     VIEGGHDSVHRLAWPRLREAGIPATILITTDMVDDGEPGQMGWGELRELASAGAAIGT
                     NGAVYRSLLGRARPLVAGDIGRARRRIVDELGAPPEIFAYPYGLHDAALRLAVAEAGF
                     IAALGQQSGAAYRASELFALPRFSVFDRFATPERFRVVVDALPLPVSELVPAEPLVEA
                     NPPDIGFTLAEGLGPAERLSCYVGGIGPVALDRLGARRIELVLPRPLPAGRTRVNCTL
                     PGPDGRWRWLGLQLLVPGGG"
     gene            6150..6740
                     /locus_tag="VGB88_03455"
     CDS             6150..6740
                     /locus_tag="VGB88_03455"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX9646527.1"
                     /translation="MSATLDHGALDIIFRNARSHSAWLGEGPDEADLRAIFELMKLGP
                     TSANSCPARFLFLRSRAAKERLRPHVSKTNETKTMTAPVIAVIGYDLAFYQHLDRLFP
                     HQNARPWFEGKDEHIRITAFRNGTLQGAYFMVAARALGFDCGPMSGFDNAGVDRTFFA
                     GTTVRSNFLCCLGRGDPAGLRPRGPRFDFAEVCTLL"
     gene            complement(6741..7307)
                     /gene="ddpX"
                     /locus_tag="VGB88_03460"
     CDS             complement(6741..7307)
                     /gene="ddpX"
                     /locus_tag="VGB88_03460"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEX9646528.1"
                     /translation="MHLVAIVPPAFDLDLDLAYARPDNFTGAAVYARAACYLHPDAAL
                     RLERAVALAAGLGLRLRIFDGFRPAEAQWALWRHTPDPDFLADPRRGSPHSRAVAVDL
                     TLARGDGTALAMGTPFDAFTPLAHHGADGLAPEARHNRLLLLGLMTDAGWDFYRNEWW
                     HYQLFESRRYPVLGDRAAGTRLIAPARP"
     gene            7391..8059
                     /gene="tsaB"
                     /locus_tag="VGB88_03465"
     CDS             7391..8059
                     /gene="tsaB"
                     /locus_tag="VGB88_03465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9646529.1"
                     /translation="MKLLALDAAAGACSVALWRDGAVLAHRYCVRARGHAEVLVPMVE
                     EVMAAAGLAYAELDGFAVTRGPGTFTGIRIGLACARGLALAAHLPLVGLTTLEVLAAG
                     VPDALAEGAVLAALEARRGEVYAQAFGPGLVPLSEPAALLPEVALALVADRDAALTGD
                     AAPRLARLLARPDLVVPGDGQPDARVLARLAAARPLPAPGVAVAPLYLRPPDARKLSE
                     STRC"
     gene            complement(8069..8341)
                     /locus_tag="VGB88_03470"
     CDS             complement(8069..8341)
                     /locus_tag="VGB88_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX9646530.1"
                     /translation="MTGTAPVGAARPAAGAERQLDITHLVCPMTFVRTKLTLEAMAPG
                     ETLSVRLNAGEPLDNVPRSVREMGHEVLSLEPAEDGHSHILRIAVR"
     gene            8459..8923
                     /locus_tag="VGB88_03475"
     CDS             8459..8923
                     /locus_tag="VGB88_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9646531.1"
                     /translation="MVTERVSELTKALSHVEQVCLDKGLKMTEQRRVIARVLSDSEDH
                     PDANAVYRRATRIDPRISMATVYRTVRLFEEAGVLERHDFGDGRSRYEEVSEEHHDHL
                     IDVDSGRVIEFQNDEIEKLQREVARKLGYRLVDHRLELYGVPADKPEDADED"
     gene            8983..9804
                     /locus_tag="VGB88_03480"
     CDS             8983..9804
                     /locus_tag="VGB88_03480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX9646532.1"
                     /translation="MTAAETFDDPFAGIHAGSLEVRLARTPEEVDAAQALRYRVFYDE
                     MGAVPTAAMRACRRDVDVHDASFDHLLVIDHERCHPTDSVVGTYRLNRRSVALRCGGF
                     YSAGEYDLSDLLAVEGEIMELGRSCVDTELRNRATMALLWRGIAAYVFYYDIGLMFGC
                     ASMSGTDPDRLALPLSYLHHHHLAPAGLRPRALPGLYTAMDRLAAAELDVREARLALP
                     PLIKGYLRLGGFVGDGAVIDRQFRTIDVCVVVKTDWVTSKYRHHYGADTYRPVPV"
     gene            9831..10682
                     /locus_tag="VGB88_03485"
     CDS             9831..10682
                     /locus_tag="VGB88_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9646533.1"
                     /translation="MTGLPAMLDPLPHLRAALLAVALALWTAAMIPVQAMVRALGSRL
                     AWVVPGVYHRGVCRLIGLEVLSHGAPIRDRPALYVANHASYLDISVLLTLVKGSFVAK
                     AEVAGWPVFGLLARLAGTVFVDRRPSRSHAPRTQMRARLEAGDRLILFPEGTSTDGNR
                     VLPFNSTFFGVVEQPIAGRVVPVQPISIAYTRYNNLPMRRRERPFLAWYGGMGMIGHL
                     WWLLCDGPATVEVAFHAPLTIADHPSRKILAADCQRRVHDGVSRLLAGRPLDPEAGAP
                     AAGRCPN"
     gene            10826..>11029
                     /locus_tag="VGB88_03490"
     CDS             10826..>11029
                     /locus_tag="VGB88_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646534.1"
                     /translation="MDVSDITIYIAKTERNTFLAATNESPYFCVEADSEEELLRLAAY
                     ALRFYRNNKDTPSQRTSSSQTIEG"
BASE COUNT         1586 a         3938 c         3893 g         1612 t
ORIGIN      
        1 cagctcacgg ccaacacccg cgaggtgacc gccggcctgg tcgccgccgg catcgacccg
       61 gtgcgcgaca tcgtgttcaa ccagagccag gtgccggccc atgccgagct cgcctggatc
      121 ttcaactgcg tcgcccggct cggctggctc aaccgcatga cccagttcaa ggagaaggcc
      181 ggcaagcacc gcgagaacgc caccgtcggg ctgttcgcct atcccaacct gatggccgcc
      241 gacatcctgc tctacaaggc gacccacgtg ccggtcggtg acgaccagaa gcagcacctg
      301 gagctcgccc gcgacatcgc caacgccttc aacagcatgt tcggggtcga cttcttcccg
      361 gcgcccgagc cgatgatcct ggacgccgcg gcgcgggtga tgagcctgcg cgacggcacc
      421 aagaagatga gcaagtcgga cccgtccgac tactcgcgcc tcaacatgac cgattcggcg
      481 gaccagatcg cgctcaagat ccgcaaggcg cgcaccgacc ccgacccttt gcccgccacc
      541 cccgatgggc tcgcaagccg gcccgaggcg gccaacctgg tgggcatcta cgcggccctc
      601 gccgaccgtt cggtggcgga ggtgtgcgcg gagttcgcgg gcgcgccgtt ctcggacttc
      661 aaggcccggc tgaccgatct cgcggtggcg acgctggggc cgatcggcgc cgagatgaag
      721 cgcctggccg ccgaccccgg ggcgatcgat tcggtgctgc gcgacggcgc gcatcgggcg
      781 cgggcgctgg ccgcgccggt gctcgccgag gccaagcgga tcgtcggctt cctcagcccg
      841 tgatcccggc ttgatcgcgg cgccggggcg gaccacctga ggcggcgagc ccgggacacg
      901 gagcggcgca tgttcgacga cgacgcggac cttccggacc ggaccggcac cgccgcgacg
      961 atccccgccg aggcgccgcc ggcgccggcg gcggaggcgg gcgcgggccg cccgcgcggg
     1021 gcacggcggc gcgcggcgcg caccgccgcc gcacttctga tcgccgccct ggtgctctac
     1081 tacccggtcg gcatggtgct gacccaccgc atcgacgacg accccgactt caccttcgcg
     1141 cccgagacct ttcccgaaag cggcagccgc gcggtggcgc tggcggcggc gctgatggag
     1201 caccaggtcg acgacgccgg ctggatcccc aacaacccgt tcttctatgc ctcggcggcg
     1261 ctcgacaaca tgcccaactt ccagcagggg ctgcgcgcgt cggtgtttcg tttcgcgatc
     1321 gagatgaccg accagatcgg gcgcacgcgc ggctcgagcc aggtcgaccc cgacctcgac
     1381 atcgccgccg gcgagttcaa gttcccgggc gacgtgtgga tctgggaccc gggcgtctcg
     1441 ctgctgccgc aggcgacgtc cgagcagcgc taccggcgcg cgatccgcca cctgcgcgcc
     1501 tacaaccagc gcctcggcgc cggcaccgcg accttcgaca gccgcgccga caacctgctc
     1561 gcgacgctcg accggttcgc ctccgacctc ggctcggcgt cggccgagat cgaccagcac
     1621 atcgccgcct acaccgggct gttcgacctt accgccgacg acctgttcta caacgtcaag
     1681 ggccgcgtct acggctacta catgatcctg accgagctcg ggggcgactt cgaagccgtc
     1741 cttgccgagc gccagctgca ggcggcgtgg gcgcagatgc tggcctcgat gcgcaccgcc
     1801 gccacgctcc agccgtgggt ggtgctgaac ggcgccccgg acggccagct ggtgcccaat
     1861 cacctcgccg cgcaaggctt ctggctgctg cgcgggcgca cacagttgcg cgagatcacc
     1921 aacatcctgc tcaaatagcg actgccgccg cggcgcgcgc gaaaagccaa gaaattcctt
     1981 gatcgacgcc aatcgaggcg caataaccct aaaaaaacaa aggttttatc gcggccgggc
     2041 cgatctggat ggatatctac ctgcccgtcg ccgagatgtc ggtcaacgtg ttggtgttgc
     2101 tcggcatggg cggtgtgatc ggattcctgt ccgggctgtt cggtgtcggc ggcggcttcc
     2161 tgctgacgcc gctcctgatc ttcatcggcg tgccgccgcc ggtggcggtg gccagccagg
     2221 ccaaccagat cgccgccgcc tcggtctcgg gcctgctcgc ccactggcgg cgcggcaatg
     2281 tcgacttccg catgggctgg gtgctgctcg ccggcggcgc gctgggctcg agcgtcggcg
     2341 tctggctgtt ccgcgtcctg cgcgagatcg gccagatcga tgccgcgatc tcgatctcct
     2401 acgtcgtgtt ccttggctcg gtcggcctgt tgatggcggt ggaaagcgcc aacaccatcc
     2461 tgcggcggcg gcgcggcgcg ccccggcgca agctgcacca gcacatctgg ctgcaccggc
     2521 tgccgctcaa gctccgcttc cggcgctcga agctctacat cagcgccatc tcgccggcgc
     2581 tgatcgggct cctggtcggc gtgctcgccg cggtcatggg ggtcggcggc ggcttcgtca
     2641 tggtgccggc gatgatctac ctcttgggca tgccgacctc ggtggtgatc ggcacctcgc
     2701 tgttccagat catgttcgtc accgccatcg tcaccgtgct gcatgcggcg accaaccaga
     2761 ccgtcgacat ggtgctggcg ctgatcctgc tcgccggcgc ggtgatcggg gcccagttcg
     2821 gcagccgcta cgtcgccgcg ctgcgcggcg agcagctgcg cggcctgctg gcgctgatgg
     2881 tgctggtggt gtgtgcgaag ctcgccttcg acctggtggt catgcccgac gacccctact
     2941 cgctgacccc ggtggtccaa tgaccggcgg ggcggcagtg cggcacggcg tcgccggcat
     3001 cgcggcgctg gcggcgctgg cggcgctggt ggcgctggcc gcggggcgcg ccggggccga
     3061 ctacctggtc gccgacctca gcgaccaccg gatcgccatc acctcgggct tcaccggctc
     3121 cgaggtgctc ctgttcggca cccgcagcgg ccggggcgac gtggtggtgg tgctgcgcgg
     3181 gcccaaggcg ccgtcggtgg tctggcgcaa gctgcgcacc ctcggcatgt ggctcaaccg
     3241 ggtccaggtg aggttcgacg acgcgcccgg ctattacgcg gtcgccgcca gccggccgct
     3301 cgaccggctc gcgcccgcca agctgctcga ggataacgag atcggcttcg ccaacctgcg
     3361 cctggcggca tcccgtgtcg acccgcgcgc caatctcgcc gacttccggg aggcgctgct
     3421 gcgcagcatg gagcgggcgc ggctttaccg cgccgcgatc agcgaggtgg tgttcatcga
     3481 cgacatcctg ttccgcgcca ccttcgcctt tcccgccaac gtgccgaccg ggctctacaa
     3541 tgcccatgtc tacctcatcg acgacggcgt cctggtcagc aagcggaccg ccgagctcga
     3601 gatctccaag accggcttcg aggcggcgat gttccgcttc gccaaccacc agccgctcgc
     3661 ctacggcctg gttgccgtgc tcgtcgcggt ggtggcagga tggtcggggg gaatgatgtt
     3721 ccgcggcaga tgacggcgat ggcagtggcc gagcagcacg tcgccggccg ggtcaagttc
     3781 ctggtctgcg tcgacgacag caaggactgc cgggtggcgc tgcgcttcgc gtcgctgcgc
     3841 gcgcgcaaca ccgagggcta tctggtgctg ctgtacgtga tcgagccggg cgacttccag
     3901 cactggctcg cggtcggcaa gctgatggag gaggagaagc gcgccgaggc cgagcagatc
     3961 ctcaacgcgc tcgccgccga ggtccacgag ttcgccggca tccacccggt gctggtggtg
     4021 cgcgaggggc gcaaggccga cgagatcctc aggctgatcg acgaggaccc gaccatcaac
     4081 atcctggtgc tcggcgccgc gcccgagggc gagggctcga acgagctggt gcgcaagctg
     4141 tcgggcgagc tgaccaagcg gctcagcatc ccgctgacca tcgtccccgg caacctgacc
     4201 gatgcccagc tcgccatgct gacctgaccg gcagggcggt tgacccggcg ccggcaatac
     4261 ccaagtaagt cattgggccc tggccgtggc gtgcgctaga ctggcgcgcg acagcgattt
     4321 ggaggcgcga ggacgatgtt tatccagacc gagccgacgc cgaacccggc aaccatgaag
     4381 ttcctgccgg gcgagccggt gctggcggac ggcaccgcca acttcaccga cgccgaggcg
     4441 gccgggcgct cgccgctggc cgggcgcctg ttccagatcc cgggcatcga gggcgtgttc
     4501 ttcggctcgg acttcgtcac cgtgaccaag tcggacgcgg ccgagtggca gctgctgaag
     4561 ccgtcgatcc tgggcgtgat catggagcac tacgtctcgg gcgcgccgat catggtcgcc
     4621 ggcgccggcg gcgaggccgg cgcccggggc cccgaggcgg ccgaggccga cgccgagatc
     4681 gtcgcccaga tcaaggagtt gctcgatacc cgggtgcgcc cggcggtggc ccaggacggc
     4741 ggcgacatca tcttccacgg ctacgagtcg ggaatcgtgc tgctggagat gcacggctcc
     4801 tgctcgggct gcccgagctc gaccgccacc ctcaagcacg gcatcgagaa catgctccgc
     4861 cactacatcc ccgaagtggt cgaggtgcgc gcgatcatgt agcgcgcagc gcgccgggcc
     4921 cgccccgcgc tcagccgccg cccgggacca ggagctgcag gccgagccag cgccagcgcc
     4981 cgtccgggcc gggcagggtg cagttgacgc gggtgcgccc cgccggcagc ggccggggga
     5041 gcacgagctc gatccgcctg gcgccgagcc ggtcgagcgc caccgggccg atgccgccga
     5101 catagcacga caggcgctcg gccggcccca ggccttcggc cagggtgaag ccgatgtcgg
     5161 gcgggttggc ctcgaccagc ggctcggccg gcaccagctc gctgaccggc agcggcaggg
     5221 catcgaccac cacccggaat cgctcaggcg tggcgaagcg gtcgaacacc gagaacctgg
     5281 gcagcgcgaa cagctcgctc gcccgatacg ccgcccccga ctgctggccg agggcggcga
     5341 tgaagccggc ttcggcaacc gccagcctca gcgcggcgtc gtgcagcccg tacgggtagg
     5401 cgaagatctc gggcggcgcg ccgagctcgt cgacgatgcg ccggcgcgcg cgcccgatat
     5461 cgccggcgac cagcggccgg gcgcggccaa ggaggctgcg gtagacggcg ccgttggtgc
     5521 cgatcgccgc ccccgccgag gccagctcgc gcagctcgcc ccagcccatc tgcccgggct
     5581 cgccgtcgtc gaccatgtcg gtggtgatca ggatggttgc ggggatgccc gcctcgcgca
     5641 ggcggggcca ggccagccgg tggaccgagt cgtggccgcc ctcgatcacg atcgcgaccg
     5701 ttcgctcggg cagcgccgcg ccgccggcga gcgcggcgac cacgcgcggc agcggcagca
     5761 ccgcatagcc gccgccggca agatgggcga gatgggcgtc gagctcggcg agcgtggtgg
     5821 tcagcggccc gcccacggcg gcgaagcgct gataggtgag gatgaccgcc gagtcctgcg
     5881 cctgcgccca gcccgcggcg ccggtcgccg cgagcgccag cgcacagcca agggcaccgc
     5941 gcccgaggcg ccgcgcaagt ccggaacggc caaccctgcg accggcggct gacatggcgt
     6001 tatcgaggcc ggctccagtg ccgaccgggc aatacttagc cagccatcga cggcgtgccc
     6061 aagtggcccg gttttcgcgc ctcatgccga tagtgtatca atagcgcggc gatgcgcgga
     6121 cgattctgcg cgcggcatgg aggcgagcga tgagcgcgac actcgaccac ggcgccctcg
     6181 acatcatctt ccgcaacgcc cgcagccata gcgcctggct cggcgagggg cccgacgagg
     6241 cggacctgag ggcgatcttc gagctgatga agctcggccc gaccagcgcc aactcgtgcc
     6301 cggcgcggtt cctgttcctg cgctcgcgcg cggccaagga acggctcagg ccgcacgtgt
     6361 cgaagaccaa cgagaccaag accatgaccg cgccggtgat cgccgtgatc ggctacgacc
     6421 tggcgttcta ccagcacctc gaccggctgt tcccgcacca gaacgcgcgc ccgtggttcg
     6481 agggcaagga cgagcacatc cggatcaccg ccttccgcaa cggcaccctt cagggcgcct
     6541 acttcatggt cgcggcgcgc gcgctcggct tcgactgcgg gccgatgtcg gggttcgaca
     6601 acgccggcgt cgaccgcacc ttcttcgccg gcaccacggt caggtcgaac ttcctgtgct
     6661 gcctcggccg cggcgatccc gccggcttgc gcccgcgcgg cccgcggttc gacttcgccg
     6721 aggtgtgcac gctgctgtga tcagggccgg gcgggcgcga tcaggcgggt gccggcagca
     6781 cggtcgccga gcaccggata gcggcgggac tcgaacagtt ggtaatgcca ccactcgttg
     6841 cgatagaaat cccagccggc gtcggtcatc agcccgagca gcagcagccg gttgtgccgc
     6901 gcctcgggcg ccaggccatc ggcgccgtga tgggcgagcg gcgtgaaggc atcgaacggc
     6961 gtgcccatcg ccagcgccgt gccgtcgccc cgggccaagg tcaggtcgac cgcgaccgcg
     7021 cgcgaatgcg gcgagccgcg gcgcggatcg gcgaggaagt cgggatcggg ggtatggcgc
     7081 cacagcgccc actgcgcctc cgccgggcgg aagccgtcga agatgcgcag cctgagcccg
     7141 aggccggcgg ccagcgccac cgcgcgttcg agcctgagag ccgcgtccgg gtgcaggtag
     7201 caggcggcgc gggcatagac ggctgcgccg gtgaagttgt cgggccgggc ataggcgagg
     7261 tcgaggtcga gatcgaacgc gggcgggacg atggcaacca ggtgcacggc gcgggcgggg
     7321 gctgggctcg gtcggggccg gccacactac accgcccgcc gtgcccggcg atagctgcgc
     7381 gtcgccggcc atgaagctgc tcgcgctcga tgccgcggcc ggcgcctgct cggttgcgct
     7441 gtggcgcgac ggcgccgtcc tcgcccaccg ctattgcgtg cgcgcgcgcg gccatgccga
     7501 ggtgctggtg ccgatggtcg aggaggtgat ggcggcggcc gggctcgctt atgccgagct
     7561 cgacggcttc gccgtgaccc gcggccccgg caccttcacc ggcatccgca tcgggctcgc
     7621 ctgcgcgcgc gggctcgcgc tcgccgcgca cctgccgctg gtcgggctga ccactctcga
     7681 ggtgctcgcc gccggcgtgc ccgacgctct tgccgagggt gcggtgctgg ccgcgctcga
     7741 ggcgcgccgg ggcgaggtct atgcccaggc gttcggcccc ggcctcgtgc cgctgtcgga
     7801 gcccgcggcg ctcttgccgg aagtggcgct ggcgctggtc gcggaccgcg atgcggcgct
     7861 caccggcgac gccgcgccga ggctcgcgcg cctgctcgcc cgaccggacc tggtggttcc
     7921 cggcgacggc cagcccgatg cccgggtgct ggcgaggctc gcggcggcgc gcccgctgcc
     7981 ggcgcccggg gtggcggtgg cgccgctcta cctgcgcccg cccgacgcac ggaagctgtc
     8041 cgaatcgacg cgctgttgag cgcgccgctc agcgcacggc gatgcgcagg atgtggctgt
     8101 gaccgtcctc cgcgggctcg agcgacagca cctcgtggcc catctcgcgc accgagcgcg
     8161 gcacgttgtc gagcggctcg ccggcattga ggcgcaccga cagcgtctcg cccggggcca
     8221 tcgcctcgag cgtgagcttg gtgcgcacga aggtcatcgg gcacaccaga tgggtgatgt
     8281 cgagctgccg ctcggccccc gccgcgggcc gcgcggcgcc cacgggcgcg gtgccggtca
     8341 tggcgccatg gtgggaagcc gcaacggcgc ggtcaagggc ccgcccgcgg cggcgcgccg
     8401 ggtgcggttg ttcggggccg tgcggtaaat tatactatcg gcgtgcgggt cctctggcat
     8461 ggtcaccgag cgggtgtctg agctcaccaa ggcgctctcc cacgtcgagc aggtgtgcct
     8521 cgacaagggg ctgaaaatga ccgagcagcg acgggtcatc gcacgcgtgc tgtccgactc
     8581 ggaggaccac ccggacgcca acgcggtcta ccggcgggcg acccgcatcg acccgcgcat
     8641 cagcatggcg acggtctatc gcaccgtgcg cctgttcgag gaggcggggg tgctcgagcg
     8701 ccacgacttc ggcgacggcc ggtcgcgcta cgaggaggtg agcgaggagc atcacgatca
     8761 cctgatcgac gtcgacagcg gccgggtgat cgagttccag aacgacgaga tcgagaagct
     8821 gcagcgggaa gtggcgcgga agctgggcta ccggctggtc gatcaccggc tcgagctgta
     8881 cggcgtcccg gccgacaagc cggaagacgc ggacgaggac tgacgggtgc gccagccggc
     8941 gggccggtcc catcacgcga caatcgggag acgggcgggt cgatgaccgc ggctgaaacc
     9001 ttcgacgacc ccttcgccgg catccacgcc ggcagcctcg aggtgcggct cgcgcgcacg
     9061 cccgaggagg tggacgcggc ccaggcgctg cgctaccggg tgttctacga cgagatgggc
     9121 gcggtgccga cggccgcgat gcgcgcctgc cgccgcgacg tcgacgtcca cgacgcgagc
     9181 ttcgaccacc tgctggtgat cgaccacgag cgctgccacc ccaccgactc ggtggtcggc
     9241 acctaccgcc tcaaccggcg cagcgtcgcg ctgcgctgcg gcgggttcta ctcggccggc
     9301 gagtacgacc tgtccgacct gctggcggtc gagggcgaga tcatggagct cggacgctcc
     9361 tgcgtcgaca ccgagctgcg caaccgcgcg accatggcgc tgctgtggcg cggcatcgcg
     9421 gcctacgtgt tctattacga catcgggctg atgttcggct gcgccagcat gtccggcacc
     9481 gaccccgacc ggctggcgct gccgctgtcc tatctgcacc accatcatct ggcgccggcg
     9541 ggcctgcgcc cgcgcgcctt gcccgggctg tatacggcga tggaccggct ggctgccgcc
     9601 gagctcgacg tgcgcgaggc gcggcttgcg ctgccgcccc tgatcaaggg ctatctgagg
     9661 ctcggcggct tcgtcggcga cggcgcggtc atcgaccgcc agttccggac catcgacgtg
     9721 tgcgtcgtgg tcaagaccga ctgggtgacg tcgaagtacc gccatcacta cggggccgac
     9781 acctaccgcc cggtgccggt ctagggcggc gcgccgggcc tgcggcggcg gtgaccggat
     9841 tgcccgccat gctcgatccc ttgccccatc tgcgcgccgc cctgctggcg gtggcgctcg
     9901 cgctgtggac cgcggcgatg atcccggtcc aggcgatggt gcgcgcgctc ggctcgcgcc
     9961 tcgcctgggt ggtgcccggc gtctaccacc ggggcgtctg ccgcctgatc ggcctcgagg
    10021 tgctcagcca cggcgccccg atccgcgacc ggccggcgct gtacgtcgcc aaccacgcct
    10081 cctatctcga catctcggtg ctgctgaccc tggtcaaggg ctcgttcgtg gccaaggccg
    10141 aggtcgccgg ctggccggtg ttcgggctgc tggccaggct cgccggcacc gtgttcgtcg
    10201 accgccgccc gagccgcagc cacgcgccgc gcacccagat gcgcgcccgg ctcgaggccg
    10261 gcgaccggct gatcctgttc cccgagggca ccagcaccga cggcaaccgc gtgctgccct
    10321 tcaacagcac cttcttcggc gtcgtcgagc agccgatcgc cggccgcgtg gtgccggtgc
    10381 agccgatctc gatcgcctat acccgttaca acaacctgcc gatgcgccgg cgcgagcgcc
    10441 cgttcctcgc ctggtacggc ggcatgggca tgatcggcca tctgtggtgg ctgctgtgcg
    10501 acgggccggc cacggtcgag gtcgccttcc atgcgccgct cacgatcgcc gaccacccct
    10561 cgcgcaagat cctggccgcg gactgccagc ggcgggtcca cgacggcgtc tcgcgcctgc
    10621 tcgccggccg cccgctcgac cccgaagccg gcgcgcccgc cgccgggcgc tgtccgaatt
    10681 agggacaggg cgtcctagtt tggagtgtgg agcgtcctta tatggattca ccgtatatgt
    10741 ggaaaatatg tcgatatatg ggatagaatg tggatagcta gaggcgtgcg ggagagagtc
    10801 tctgccaacc tgagtggaga acaccatgga cgtgtcagat atcaccatct atatcgccaa
    10861 aacagagcga aatacatttt tggcagccac aaatgaatcg ccatacttct gtgtagaggc
    10921 ggacagtgag gaagagctat tgcgccttgc ggcgtatgct ttgagatttt atcgaaacaa
    10981 taaagacacg ccatcacagc gtacatcgag ttctcaaact attgaggga
//
LOCUS       DASWHU010000100        16964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2626176, whole genome shotgun sequence.
ACCESSION   DASWHU010000100 DASWHU010000000
VERSION     DASWHU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16964
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626176"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1884
                     /gene="recQ"
                     /locus_tag="VGB88_03495"
     CDS             76..1884
                     /gene="recQ"
                     /locus_tag="VGB88_03495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX9646535.1"
                     /translation="MAAAERVLEGVFGFRAFRPGQAAVVEAVLAGRDVLAVMPTGSGK
                     SLCYQVPALVLGGLTVVVSPLVALMQDQVAALRLAGVAADAINSSRARADNVAAWRRA
                     AAGETRLLYMAPERLMTERMLAALAELPVTLFAVDEAHCVSQWGPAFRPEYEALGRLR
                     ELFAGAPIAALTATADEITRGDIAAKLLRPDARVFVSGFDRPNIRLSVAMKRDWKGQL
                     VRYVEQRKGASGIVYCLSRKKTEAAAELLAAAGVTALPYHAGMDKAAREANQDRFMSE
                     AGVVMVATIAFGMGIDKADVRFVLHTDLPGNVEAYYQEIGRAGRDGAPADAHMLYGLD
                     DIRMRRRFIEQDGGGDERLRREHKRLDALIGYCESPRCRRRLLLAYFGEDAGDCGNCD
                     MCLDPVELVDGAEDAAKVLDAVLRTGQLFGAAHITDILCGARTEKVVRSRHDRLAAFG
                     AGADHGKEDWRSIIRQMVAAGLLLVDIAGHGGLSATGQGRAVVDGRTGFRYRRDAMPQ
                     PARRRAKAAPAEAPAAGAPSRADEALLQALKALRLRLARERGVPAYVIFPDRSLVDMV
                     RRRPGTAAEFAVVDGVGEVKLRAFADAFLAAIAVHG"
     gene            2066..2905
                     /gene="rpsB"
                     /locus_tag="VGB88_03500"
     CDS             2066..2905
                     /gene="rpsB"
                     /locus_tag="VGB88_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9646536.1"
                     /translation="MAVPTFTMRQLLEAGVHFGHQTHRWNPKMEPYLYGVRNGVHIID
                     LQQTVPLLYRALEFVEQVVGRGGRVLFVGTKRQAQEPLAEAAKRCGQYFVNHRWLGGM
                     LTNWRTISNSIGRLRTLDEQLAGEQSGLTKKELLRLTRERDKLERTLGGIREMGGLPD
                     VVFVVDTNKEEIAVQEAVKLEIPVVAVLDSNSNPAGITYPIPGNDDATRSVTLYCELI
                     AQSVLNGLQAEQTARGVDIGEAEEAPAENLAEAVPAVPETAATQPAAGEDEPKAETGQ
                     PSA"
     gene            2939..3862
                     /gene="tsf"
                     /locus_tag="VGB88_03505"
     CDS             2939..3862
                     /gene="tsf"
                     /locus_tag="VGB88_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9646537.1"
                     /translation="MAQITASLVKELRDKTGAGMMDCKRALTETGGDLEQAVDWLRKK
                     GLSAAARKAGRVAAEGLIGISVSDAAGAVVEVNAETDFVARNEEFQDFVRTVAEVAGT
                     VGGDLERLKAAPYPGGASVGERLTEMIGRIGENLVVRRAAAVTVGTGVVASYVHNAAA
                     PGLGKIGVLVGLDSAGDKEALAAFGKQLAMHIAAARPEAVSRDDVDPALLARERDILA
                     AQARASGKPEAIIDKMVEGRLRKFYEDVVLTEQTFVIDGESRVGDAIAKAADAAGAPI
                     TVAAMVRFELGEGIEKEASDFAADVAAQARR"
     gene            3992..4741
                     /gene="pyrH"
                     /locus_tag="VGB88_03510"
     CDS             3992..4741
                     /gene="pyrH"
                     /locus_tag="VGB88_03510"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9646538.1"
                     /translation="MPSKEAVQAVPAARYGRVLLKLSGEALMGEVAYGIDPQAVARIA
                     HDVRAVHDKGVEVCLVVGGGNIFRGLAGVAAGIDRATADGMGMLATIINALALQSTLE
                     GLGLTTRVVSAIPMQTICEPYIRRRAVRHMEKGRVVICAAGTGNPYFTTDTAAALRAA
                     ELGCQGLFKGTQVDGVYSSDPKSDRAAVRFDRISYSRVLAEDLKVMDATAISLARDNG
                     IPVIVFSIHSPGAFAEVVCGQGRFTMVSEEE"
     gene            4746..5303
                     /gene="frr"
                     /locus_tag="VGB88_03515"
     CDS             4746..5303
                     /gene="frr"
                     /locus_tag="VGB88_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9646539.1"
                     /translation="MPAFDMDDLRRRMDGALHVLKKEYAGLRTGRASVSLLEPVTVAA
                     YDSEMPLSQLATISAPEPRLLSVQVWDRSLAKAVETAIRASGLGLNPIGEGQLIRVPI
                     PELTEERRKELAKIAHKYAEEARVAVRNVRRDGMDSLKALEKSGDISQDEHHERGVEV
                     QELTDAMIAEIGQVMEEKEKEILQV"
     gene            5374..6048
                     /locus_tag="VGB88_03520"
     CDS             5374..6048
                     /locus_tag="VGB88_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX9646540.1"
                     /translation="MDGNGRWAVSRGLPRITGHQRGAEAVRVTVRACRELGIEYLTLY
                     AFSSENWKRPADEVEDLMALLRLYLQSELDELAANGVRLRVIGDLSALHRDIQDLIAR
                     AQAATRDNDALTLTVALNYGGRNEIVAAVRDIADEVAGGRLRPDQITAELFAHHLQTV
                     GIPDPDLIVRTSGEHRLSNFLLWQCAYAEFVFTPVLWPDFGRRELEQSIAEYRRRERR
                     YGGAGA"
     gene            6029..6859
                     /locus_tag="VGB88_03525"
     CDS             6029..6859
                     /locus_tag="VGB88_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9646541.1"
                     /translation="MAVPAPDPSTGGDFWRRLATAAVAAPLALGGVWLGGLALAALVA
                     AAAAIMAHEWWQLAGVEPAPAALAVLTAAVVGAVVLAALGLYGFGVVMLAGAAAMGGL
                     LAGGGWRRRLGAATAAAYVGLPCLALLWLRARPDDGLALVLWLLALVWATDIAAYVTG
                     RLVGGPKLAPRLSPAKTWAGLAGGLAAAVAITGVVGGLAGRGDLAAVLAAAAGLAAIA
                     QGGDLFESWLKRRRGMKDSGTLLPGHGGLLDRVDGLIAAAPAMTMLVIASGGEVRPWP
                     "
     gene            6850..8046
                     /locus_tag="VGB88_03530"
     CDS             6850..8046
                     /locus_tag="VGB88_03530"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9646542.1"
                     /translation="MAMIAAREAAPPRRVTILGSTGSVGCNTVSLIEANPGAYQVEAL
                     TAQRNVELLAAQARRLRPRLAVIGEERLYGALKAALEGTNVRAAAGPGALVEAAMLPA
                     AWVMAAIVGAAGLSPTLAAVRRGAVVALANKECLVCAGKIMVAEVTRHGATLLPVDSE
                     HNAIFQVFDFTHPERVERIILTASGGPFRQLDRAAMARVTPAQAVLHPNWAMGAKISV
                     DSATMMNKGLEMIEAYHLFPIGAEQIEVLVHPQSVVHSMVAYCDGSVLAQLGAPDMRT
                     PIAYSLAWPDRIDAPVPRLDLAALYQLTFEAPDFERFPALALARHALSNGAAAATVLN
                     AANEVAVHGFLDGRIGFLDIVATVERTLEQVTPGPPANLDDVVEIDRSAREVASRLIG
                     ARGSGS"
     gene            8079..9212
                     /gene="rseP"
                     /locus_tag="VGB88_03535"
     CDS             8079..9212
                     /gene="rseP"
                     /locus_tag="VGB88_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX9646543.1"
                     /translation="MDTLISFGHTILSFLVVFTVVVFVHEFGHYWVARRCGVRVEVFS
                     IGFGRELFGITDRAGTRWKLGLVPLGGFVRFFGDENAASRPALGLDRMTPEDRSVSFH
                     YKPLGQRALIVAAGPLANFVLSIVLLTSLFVTVGRPITPAVVNEVLPDSAAMAAGFQP
                     GDRVVSIGDTAIERFEELREIVMFSPGVAMAMVVERAGKMVPIQVTPEDVEITDSLGN
                     PHHIGRLGISVAAREFVRLDPLRGVAAAVVDTYDLVARSLQGLGEILIGKRDSAELGG
                     PIMIAQMSGERAEAGALALIEFVVILSATLGLINLFPIPLLDGGHLAFYAFEAVRGRP
                     LGMRAQELGYMVGLAVVVTLMVFATVNDLSRPAVIDFFTRLVG"
     gene            9217..11517
                     /gene="bamA"
                     /locus_tag="VGB88_03540"
     CDS             9217..11517
                     /gene="bamA"
                     /locus_tag="VGB88_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX9646544.1"
                     /translation="MVGGRTAAAARALVWLAAVALGVLAAAPARAQQPGLGDVIAAIR
                     VEGNQRIEDATVVSYMKVVAGDRFDPARVNESLKNLFETGLFADVALAREGNTLVVRV
                     VENPIINRLAFEGNRRIEDDVLSGEVQLKPRTVYTQTRVQGDVARILEIYRRAGRFAA
                     TVDPKVIQLPQNRVDLVFEINEGPLTGVQRIVFIGNRNFGNATLREVIQTKQTRWWRF
                     FSTTDSYDPDRLSFDRELLRRFYLKEGFADFRVNSAVAELTPDRSAFFVTFTVEEGER
                     YRFGDIDVVNELPGFDPETVRDDVTTDSGDWYNADEVEETVQDLTERIGALGYAFVDV
                     APEVRRRREDLAIDVTYKIAEGERIYVQRIDITGNVRTLDRIIRRNMRLAEGDAFNAA
                     RIRRSRTLIQGLDFFSRVDINNRPGDSPDQTIVTVDVEEQSTGEVTFGGGFSSTEGPT
                     GSIGVRERNLLGRGQDLSLQFLISGVTQDLDLSFTEPYFLNRDFAAGIDVFRREREFQ
                     NASFDRRDLGFSLRGTYPLSEYLSQTVRYTLAQEEILPFFGASNVVQEQAGERLISRV
                     DQSLLYDRRDNRIEPTDGYFGRWSVGIAGLGGDKHFLKQSVDAGYYYSFWDDWVASTL
                     AQAGYVFSLQSDEKVDISNRFFLGGNNFRGFAPAGVGPRELSDNTALGGKITYTGTLE
                     VAFPIGLPNELGVKGRLFTIAGSLSELDNETPTTIDTGSLRMSVGFGVSWASPFGPVR
                     VDLATAVVKEDFDETELISFGFGSFF"
     gene            11675..12151
                     /locus_tag="VGB88_03545"
     CDS             11675..12151
                     /locus_tag="VGB88_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9646545.1"
                     /translation="MIAIGVVDLQKIMRESVAAQKVRSEIEAKQTAYREQISARENEL
                     RTQQQELERQRTILSAEAFAEREKEFYKKVEGLQRDVAERNKQLEESLAYARDQVQGT
                     ALRIIAEIADQRKLGLVLDKSQLLLVAKGLEFSDQALERLNAELPSVSTTPPPRED"
     gene            12279..13313
                     /gene="lpxD"
                     /locus_tag="VGB88_03550"
     CDS             12279..13313
                     /gene="lpxD"
                     /locus_tag="VGB88_03550"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9646546.1"
                     /translation="MADPRFYRSRGPFALGELAQSIGATLAPGADADLKLADVAALDE
                     AGPEHLSFFDNRKYLQSLRQTRAGAVVLAESARGQAPSGAALLFSAQPYYAFALAARA
                     FHPIVAVSRAGISPASHLDPSARLGEGCQVDAGAVIAAGASIGSRCRIRANAAIGEKV
                     VIGDDCDIGAGASLSHCLIGDRVILHPGVCIGQDGFGFARGGEGHLKVPQLGRVIVEN
                     DVEIGANSSIDRGSGPDTIIGPGCKIDNLVQIAHNVRLGAGCVIVAQVGISGSTQLGD
                     YAVLAGQVGIAGHLKIGKEAVLAAKSGVHSDVPAGATYGGIPARPIRQWRRMVAAMAR
                     LGRNGRGEGG"
     gene            13314..13781
                     /gene="fabZ"
                     /locus_tag="VGB88_03555"
     CDS             13314..13781
                     /gene="fabZ"
                     /locus_tag="VGB88_03555"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX9646547.1"
                     /translation="MAKAAAVAGASLIDVRRIMEMIPHRYPFLLVDRIVEVVEGVSAT
                     GVKNVTFNEPHFTGHFPGTPVMPGVLIIESMAQTAALFVVHTLGPDAEGKLVYFMTID
                     GARFRKPVVPGDTMTIRVEKLQARRNVWKFACKVKVGDILVAEATIAAMIMDM"
     gene            13781..14605
                     /gene="lpxA"
                     /locus_tag="VGB88_03560"
     CDS             13781..14605
                     /gene="lpxA"
                     /locus_tag="VGB88_03560"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9646548.1"
                     /translation="MPMPADQIEPRIHPTAIIAAGAEIGAGVAVGPYSCIGPHVELDD
                     GVDVHAHVVIEGHTQVGAGTSIFPFASIGHRPQDLKYRGEPSSLVIGRNTVIREYVTM
                     HPGTEGGGMVTRIGDNCLFMVAAHVAHDCKIGNGVVLVNNATLGGHVAIDDHAILGGL
                     SAVHQFVRIGKHAMVGGMSGVEGDVIPYGSVVGNRANLSGLNIIGLKRRGFSRDVIHD
                     LRKAYRLLFAEEGTMAERLEDVAEEFGRVEPVMEIVAFIRSDSSRAICQPKLDRAA"
     gene            14595..15455
                     /gene="lpxI"
                     /locus_tag="VGB88_03565"
     CDS             14595..15455
                     /gene="lpxI"
                     /locus_tag="VGB88_03565"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974227.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX9646549.1"
                     /translation="MPPDPASLPVAPAAGRLGVLAGGGRLPRVVLEACRRQGREVFAV
                     AFEGSTDPETVTDMPHQWVRLGAVGQTIALLKANRVEELVMAGPVARPSWAGMRPDLR
                     ALKLLPKVLAGGQGDDSILSLAIRELEAEGFRVVGAHAVVPELLAPAGPLGARVPDRG
                     ARADIARGVEVAMALGAVDVGQAVVVQQGVVLGIEAVEGTDALLGRAATLRRDGPGGV
                     LVKCRKPSQDRRADLPSIGADTVRRAREAGLRGIAVEAGETLVLDRALVIEAADAGGL
                     FVYGFEGGPG"
     gene            15491..16432
                     /locus_tag="VGB88_03570"
     CDS             15491..16432
                     /locus_tag="VGB88_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX9646550.1"
                     /translation="MTQPWPELPYAAWRDTLETLHLWTQIVGKYRLAHTPWLNHSWHA
                     TFYVSARGLSTSTIPMGERALELEFDFIDHALVARASDGARAEIGLAPISVADFHARF
                     VAMLRQLDADTFLHGVPNEIADPIPFDQDTRHASYDAGAAHRFWQSLVRIDRVFRLFR
                     TGFLGKSSPVHFFWGSFDLAVTRFSGRPAPEHPGGIPALPDPITREAYSHEVSSAGFW
                     PGGGAIDFAAFYAYAYPAPDGFAAADVAPAEAYFDAALGEFLLPYDAVRGSDRPDRAL
                     LTFLQATYAAAADLGNWDRAALDCPLGEPRVPRPLSR"
     gene            16565..>16964
                     /gene="gloA"
                     /locus_tag="VGB88_03575"
     CDS             16565..>16964
                     /gene="gloA"
                     /locus_tag="VGB88_03575"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX9646551.1"
                     /translation="MAETDGFRMLHTMVRVKDLDASLDFYTRLLGMRLLRRRDYPDGK
                     FTLAFVGYGPEESSAVLELTYNWDQEKPYDLGSGYGHVAIGVPDIYGTCDRLKEAGAK
                     VVRAPGPMKHGSTVIAFVEDPDGYKIELIER"
BASE COUNT         2507 a         5759 c         6134 g         2564 t
ORIGIN      
        1 cgtccccggc gtgatcgacg tccacgacct gtgacgcgcg cggcggcagt ctctgtgcgg
       61 tccgagcccg ccaatatggc ggcggcggag cgggtgctgg agggcgtgtt cggcttccgc
      121 gcgttccggc ccgggcaggc ggcagtggtc gaggcggtgc tcgccgggcg cgacgtgctc
      181 gcggtgatgc cgaccgggtc gggcaagtcg ctctgctatc aggtaccggc gctggtgctc
      241 ggcgggctca ccgtcgtcgt ctcgccgctg gtcgcgctga tgcaggacca ggtcgctgcg
      301 ctccgcctcg ccggggttgc ggcggacgcg atcaactcct cgcgcgcccg cgccgacaac
      361 gtcgccgcct ggcggcgtgc cgccgccggc gagacgcggt tgctgtacat ggcgcccgag
      421 cggctgatga ccgagcggat gctggccgcg ctggccgagc tgccggtgac gctgttcgcg
      481 gtcgacgagg cgcattgcgt gtcgcagtgg gggcccgcgt tccggcccga gtacgaggcg
      541 ctcggccgcc tgcgcgagct gttcgccggc gcacccatcg ccgcgctgac cgcgaccgcc
      601 gacgagatca cccgcggcga catcgcggcc aagctgctgc gcccggacgc gcgcgtgttc
      661 gtgtcgggct tcgaccggcc caacatccgc ctgtcggtgg cgatgaagcg cgactggaaa
      721 gggcagctcg tgcggtacgt cgagcaacgc aagggcgcgt ccggaatcgt ctactgcctg
      781 tcgcgcaaga agaccgaggc cgcggcagag ctgctcgccg ctgcgggcgt cacggcgctg
      841 ccctatcacg ccggcatgga caaggcggcg cgcgaggcga accaggaccg gttcatgagc
      901 gaggccggcg tggtgatggt ggcgaccatc gccttcggca tgggcatcga caaggcggac
      961 gtgcggttcg tgctgcacac cgacctgccg ggcaacgtcg aggcctacta tcaggagatc
     1021 ggccgcgccg ggcgcgacgg cgcgccggcg gacgcgcaca tgctctacgg cctcgacgac
     1081 atccgcatgc gccggcggtt catcgagcag gacggcggcg gcgacgagcg cctgcgccgc
     1141 gagcacaagc gtctcgacgc cctgatcggc tactgcgagt cgccgcgctg ccggcgccgc
     1201 ttgctgctgg cctatttcgg cgaggacgcc ggcgactgcg gcaattgcga catgtgcctc
     1261 gacccggtcg agctggtcga cggcgccgag gacgcggcca aggtgctcga cgcggtgctg
     1321 cgcaccggcc agctcttcgg cgccgcccac atcaccgaca tcctgtgcgg cgcgcgcacc
     1381 gaaaaggtgg tgcggtcgcg tcacgaccgg ctggcggcat tcggcgccgg cgccgatcac
     1441 ggcaaggagg actggcgctc gatcatccgc cagatggtcg cggcgggact tctgctggtg
     1501 gacatcgccg gccacggcgg cctgagcgcg accggccagg gccgggccgt cgtcgacggc
     1561 cggaccggct tccgctaccg gcgcgatgcg atgccgcaac ccgcgcgccg gcgcgccaag
     1621 gcggcaccgg ccgaggcccc ggcggccggc gcgccgtcgc gggcggacga ggcgctgctc
     1681 caggcgctca aggcgctgcg tctccgcctc gcgcgcgagc gcggcgtgcc cgcctacgta
     1741 atctttcccg accgttcgct ggtcgacatg gtgcgccggc ggcccggcac agccgccgag
     1801 ttcgccgtcg tggacggcgt cggcgaggtc aagctgcgcg ccttcgccga cgccttcttg
     1861 gccgcgatcg cggtccacgg ctagccgcgc gccagcgcct gcgggcttgt aaaggtcgcg
     1921 atttggcgct atctcgcgcc cggtagcaca acctcacacg cgggccttgc ggcaggcgtt
     1981 gccgtttgcc gttccggtgc ctcggtcgac ccccggtcgg cccgggcctc cgcccgcggc
     2041 ggctcaaccg gagaaggatc agaccatggc cgtgcccacc ttcaccatgc gccagctgct
     2101 cgaggccggc gttcatttcg gtcaccagac ccaccgctgg aaccccaaga tggagccgta
     2161 cctctacggg gtgcgcaacg gggtccacat catcgacctg cagcagaccg tgccgctgct
     2221 ctaccgcgcg ctcgagttcg tcgagcaggt ggtggggcgc ggcgggcggg tgctgttcgt
     2281 cggcaccaag cgccaggcgc aggagccgct ggccgaggcc gccaagcgct gcggccaata
     2341 tttcgtcaac caccgctggc tgggcggcat gctgaccaac tggcgaacga tctcgaactc
     2401 catcggccgc ctgcgcacgc tcgacgagca gctcgccggg gagcagtcgg ggctgaccaa
     2461 gaaggagctg ctccgcctga ctcgcgagcg cgacaagctg gagcgcaccc tgggcgggat
     2521 ccgcgagatg ggcgggctgc ccgacgtggt gttcgtggtc gataccaaca aggaggagat
     2581 cgcggtccag gaagcggtca agctcgagat cccggtggtc gcagtgctcg acagcaacag
     2641 caatccggcc ggcatcacct atccgatacc gggcaacgac gacgccaccc gctcggtcac
     2701 gctctactgc gagctgatcg cgcagtcggt gctcaacggg ttgcaggccg agcagacggc
     2761 gcggggcgtc gacatcggcg aggccgagga ggccccggct gagaatctgg ccgaggccgt
     2821 gccggcggtg ccagagacgg ccgcgaccca gcctgccgcc ggcgaggacg agcccaaggc
     2881 cgagaccggc caaccctccg cctgacgccc gacacggacg accgaccaga ggaagatcat
     2941 ggcacagatc acggcatcgc tggtgaagga gctgcgcgac aagaccggcg ccggcatgat
     3001 ggactgcaag agggcgctga ccgagaccgg cggcgacctc gagcaggcag tcgactggct
     3061 gcgcaagaag gggctgtcgg cggcggcgag gaaggccggc cgggtggcgg cggaaggcct
     3121 gatcgggatc tcggtgagcg atgccgccgg cgccgtggtc gaggtcaatg cggagaccga
     3181 cttcgtcgcc cgcaacgagg agttccagga cttcgtgcgc acggtcgccg aggtcgccgg
     3241 caccgtcggc ggcgatctgg agcggctgaa ggccgcgccc tatcccggcg gcgcgtcggt
     3301 cggcgaacgg ctgaccgaga tgatcgggcg gatcggcgag aacctcgtgg tgcggcgggc
     3361 ggcggcggtt acggtcggca ccggcgtggt ggcgagctac gtccacaacg ccgcggcgcc
     3421 gggcctcggc aagatcggcg tgctggtcgg gctcgactcg gccggcgaca aggaggcgct
     3481 ggcggccttc ggcaagcagc ttgccatgca catcgccgcc gcgcgccccg aggcggtcag
     3541 ccgcgacgac gtcgacccgg cgctgctcgc gcgcgagcgc gacatcctgg ccgcgcaggc
     3601 gcgcgcctcc ggcaagcccg aggcgatcat cgacaagatg gtcgagggcc ggttgcgcaa
     3661 gttctacgag gacgtggtgc tgaccgagca gaccttcgtc atcgacggcg aaagccgggt
     3721 cggcgacgcc atcgccaagg ccgccgacgc cgccggggcg ccgatcacgg tcgccgccat
     3781 ggtccgcttc gagctcggtg agggaatcga gaaggaggcg agcgacttcg ccgccgacgt
     3841 cgccgcccag gcacggcgct gacgcaccgc cgccccgtcc gcccggcgag cgtgcatcga
     3901 tcggcacaag gcgccgacat tcgatgcgaa ctcgcctata gtcgtcgcgg gcgcggtggc
     3961 gcgcatggtg cccgggaacc aatggccgaa catgccgtct aaggaagccg tccaggcggt
     4021 cccggccgcg cgctacggcc gggtcctgct caagctgtcg ggcgaggccc tgatgggcga
     4081 ggtcgcctac ggcatcgatc cccaggcggt ggcgcgcatc gcgcacgacg tgcgtgcggt
     4141 ccacgacaag ggggtcgagg tgtgcctggt ggtcggcggc ggcaacatct ttcgcggcct
     4201 cgccggtgtc gcggccggga tcgaccgcgc cactgccgac ggcatgggca tgctggcgac
     4261 gatcatcaat gcgctggcgc tgcaaagcac gctcgagggc ctcggtctga ccacgcgcgt
     4321 ggtctcggcg atcccgatgc agacgatctg cgagccctac atccgccggc gcgcggtgcg
     4381 ccacatggag aaggggcggg tggtgatctg cgccgccggc accggtaacc cgtacttcac
     4441 caccgatacc gcggccgcgt tgcgcgccgc cgagctcggc tgccagggcc tgttcaaggg
     4501 cacccaggtc gacggcgtct attcgtcgga cccgaaaagc gaccgcgccg cggtgcgctt
     4561 cgacaggatc agctactctc gggtgctggc cgaggacctg aaggtgatgg acgccaccgc
     4621 catctcgctg gcgcgggaca acggcattcc ggtcatcgta ttctcgatcc acagccccgg
     4681 ggccttcgcc gaggtggtct gcggccaggg ccggtttacg atggtaagcg aagaggaatg
     4741 aggcgatgcc ggccttcgat atggacgatc tcaggcggcg catggacggg gcgcttcacg
     4801 tccttaagaa ggagtatgcg ggcctgcgca ccgggcgggc gtcggtcagc ctgctcgagc
     4861 cggtgacggt cgcggcctac gactcggaga tgccgctcag ccagctcgcc acgatcagcg
     4921 cgcccgagcc gcggctgctg tcggtgcagg tgtgggacag gtcgctggcc aaggcggtcg
     4981 agaccgcgat ccgcgcgagc gggctcggcc tcaatccgat cggcgagggc cagctgatcc
     5041 gggtgccaat cccggagctg accgaggagc ggcgcaagga gctggccaag atcgcgcaca
     5101 aatacgccga ggaggcgagg gtcgcggtgc gcaacgtccg tcgcgacggg atggacagcc
     5161 tgaaggcgtt ggagaagtcg ggcgacatct cgcaggacga gcatcacgag cgtggcgtgg
     5221 aggtccagga gctgaccgac gccatgatcg ccgagatcgg gcaggtgatg gaggaaaagg
     5281 aaaaggagat ccttcaggtc tgaccccatg gaatcggccg catcccgaca cgcgtcgccc
     5341 ccgccgccac cgccgcgaca cattgcgatc atcatggacg gcaacggccg ctgggccgtc
     5401 agccgggggc tgccgcgcat caccggccat cagcgcggcg ccgaggcggt gcgggtgacg
     5461 gtgcgggcct gccgcgagct cggcatcgag tacctgaccc tgtacgcgtt ctcgtccgag
     5521 aactggaagc ggccggcgga cgaggtcgag gacctgatgg cgctgctgcg cctctatctg
     5581 cagagcgaac tcgacgagct tgccgccaac ggcgtgcggc tcagggtgat cggcgatctg
     5641 tccgcgctcc accgcgacat ccaggacctg atcgcccgcg cccaggccgc gacccgcgac
     5701 aacgacgcgc tcaccctgac cgtcgcgctc aactatggcg gccgcaacga gatcgtcgcc
     5761 gcagtgcgcg acatcgccga cgaggtcgcg ggcggccggc tgcggcccga ccagatcacc
     5821 gccgagctgt tcgcgcacca cctccagacc gtcggcatac cggacccgga cctgatcgtc
     5881 cgcaccagcg gcgagcaccg gttgagcaat ttcctgctgt ggcagtgcgc ctatgccgag
     5941 ttcgtcttca cgccggtgct gtggcccgac ttcgggcgcc gggagctcga gcaatcgatc
     6001 gccgagtatc ggcggcgcga acggcgctat ggcggtgccg gcgcctgatc cttccaccgg
     6061 cggcgacttt tggcgccgac tggcgacggc cgcggtcgcg gcgccgctgg cgctgggcgg
     6121 ggtgtggctg ggcgggcttg cgctggccgc gctggtcgcg gcggcggcgg cgatcatggc
     6181 ccatgaatgg tggcagctcg ccggagtcga gccggcgccg gcggcgctgg ccgtgctgac
     6241 ggccgcggtg gtcggcgctg tggttcttgc cgcgctcggg ctatatggtt ttggagtggt
     6301 aatgctcgcg ggggcggcgg caatgggcgg gttgctcgcc ggcggcggct ggcggcggcg
     6361 cctcggcgcg gcgaccgccg cggcctatgt cggcctgccg tgcctggcgc tgttgtggct
     6421 gagggcacgc ccggacgacg gcttggcgct ggtgctgtgg ctgctggcgc tggtttgggc
     6481 gaccgacatc gcggcttacg tcacgggccg cctggtgggc gggccgaagc ttgcgccgcg
     6541 gttgagcccg gccaagacct gggccggcct cgccggggga ctcgcggccg cggtcgcgat
     6601 caccggcgtg gtcggcggcc tcgccggccg cggcgatctg gcggcggtgc tggcggcggc
     6661 cgccgggctc gccgcgatcg cccagggcgg ggacctgttc gaatcgtggc tcaagcgacg
     6721 gcgcggcatg aaggattcgg gaacgttgct gccgggacac ggtggtctgc tcgatcgggt
     6781 cgacggattg attgcggcgg ccccggctat gactatgttg gtgatcgcat ctgggggtga
     6841 ggttcggcca tggccatgat cgcagcgcgc gaggcggcac cgccgcgccg ggtgacgatc
     6901 ctaggatcga ccggctcggt cggctgcaac accgtcagcc tgatcgaggc caacccgggc
     6961 gcctatcagg tcgaggcgct caccgcgcag cgcaacgtcg agctgctggc cgcccaggcg
     7021 cgccggttgc gcccgcggct cgcggtgatc ggcgaggagc gcctttacgg cgcgctgaag
     7081 gcggcgctcg aagggaccaa cgtgcgcgcc gccgctgggc ccggcgcgct ggtcgaggcg
     7141 gcgatgctgc cggctgcgtg ggtgatggcg gcgatcgtcg gcgcggcggg gctgtcgccg
     7201 accctggcgg cggtgcgccg cggcgcggtg gtggcgctgg ccaacaagga atgcctggtg
     7261 tgcgcgggca agatcatggt cgccgaggtg acgcgccacg gcgccaccct gctgccggtc
     7321 gattccgagc acaacgcgat cttccaggtg ttcgacttca cccatcccga gcgggtcgag
     7381 cgaatcatcc tgaccgcgtc cggcgggcca ttccgccaac tcgaccgcgc cgccatggcc
     7441 cgggtcaccc cggcgcaagc ggtgctgcat cccaattggg cgatgggcgc caagatctcg
     7501 gtcgactcgg cgaccatgat gaacaagggc ctcgagatga tcgaggccta tcacttgttc
     7561 ccgatcggcg ccgagcagat cgaggtcctg gtccacccgc agtcggtggt gcacagcatg
     7621 gtcgcctatt gcgacggctc ggtgctggcg cagctcggcg cgcccgacat gcgcacgccg
     7681 atcgcctaca gcctggcctg gccggaccgg atcgatgcgc cggtcccgcg gctggacctg
     7741 gcggcgctct accagctcac cttcgaggcg cccgatttcg aacgcttccc cgcgctcgcc
     7801 ctggcgcgcc acgcgctgag caatggcgcc gccgcagcca ccgtgctgaa cgccgccaac
     7861 gaggtggcgg tgcacggttt cctggacgga cggatcggtt ttctcgatat agtcgccacg
     7921 gtcgagcgca ccctcgagca ggtgaccccc ggcccgcccg ccaatctcga cgacgtggtc
     7981 gagatcgacc gctcggcgcg agaggtcgca agccgcttga tcggcgcgcg cggctcgggc
     8041 agctagacgg aaacggcaca agaacgacgt cgcgcggcat ggacacactg atcagcttcg
     8101 ggcacacgat cctttcgttc ctggtggtgt tcaccgtcgt ggtgttcgtc cacgagttcg
     8161 gccattactg ggtggcgcgc cggtgcgggg tccgggtcga ggtgttctcg atcggcttcg
     8221 gccgcgagct gttcggcatc accgacaggg ccggcacgcg ctggaagctg ggcctggtgc
     8281 cgctcggcgg cttcgtccgc ttcttcggcg acgagaacgc ggccagccgc ccggcgctcg
     8341 gcctcgatcg gatgactccc gaggaccggt cggtgtcgtt ccattacaag ccgctgggcc
     8401 agcgcgcgct gatcgtcgcc gccggaccgc tggccaactt cgtgctgtcg atcgtcctgc
     8461 tgaccagcct gttcgtcacg gtcggccggc cgatcacgcc cgcggtggtc aacgaggtgc
     8521 tgccggattc ggcggccatg gcggcgggct tccagcccgg cgaccgcgtg gtcagcattg
     8581 gcgacaccgc gatcgagcgg ttcgaggagc tgcgcgagat cgtcatgttc agccccggcg
     8641 tggccatggc catggtcgtc gagcgcgccg gcaagatggt gccgatccag gtcacgccgg
     8701 aggacgtcga gatcaccgac agcctcggca acccccacca tatcgggcgc ctcggcatca
     8761 gcgtcgcggc gcgcgagttc gtccgcctcg atccgttgcg cggggtcgcg gcggccgtgg
     8821 tcgacaccta cgacctggtg gcgcggtcgc tgcagggcct gggcgagatc ctgatcggca
     8881 agcgcgattc ggccgagctc gggggcccga tcatgatcgc ccagatgtcg ggcgagcggg
     8941 ccgaggcggg cgccctggcc ctgatcgagt tcgtggtcat cctgtcggcg acgttgggat
     9001 tgatcaacct gttcccgatt cctctgctcg acggcggcca tctggcgttc tatgccttcg
     9061 aggcggtgcg cggccggccg ctcggcatgc gcgcccagga gctcggatac atggtcggct
     9121 tggcggtggt ggtgacgttg atggtgttcg ccacggtcaa cgatctcagc cggccggcgg
     9181 tgatcgactt cttcaccaga ctcgtgggct agtctcgtgg tcggggggcg gacggcggca
     9241 gcggcgcggg cgctggtttg gctcgcggcc gtggcgttgg gcgtgctggc ggcggcgccg
     9301 gcgcgcgcgc agcagcccgg gctcggcgac gtgatcgcgg cgatccgggt cgagggcaac
     9361 cagcggatcg aggacgcgac cgtcgtctcg tacatgaagg tggtggccgg cgaccggttc
     9421 gacccggcgc gggtcaatga gtccctgaag aacctgttcg agaccgggct gttcgccgac
     9481 gtggcgctgg cgcgcgaggg caacacgctg gtggtgcggg tggtcgagaa cccgatcatc
     9541 aaccgcctgg cgttcgaggg caaccggcgc atcgaggacg acgtgctcag cggcgaggtc
     9601 cagctcaagc cgcgcacggt ctacacccag actcgggtgc agggcgacgt cgcccgcatt
     9661 ctcgagatct accggcgcgc cggcaggttc gcggcgaccg tcgatccgaa agtgattcag
     9721 ctgccgcaga accgcgtcga cctggtgttc gagatcaacg aggggccgct gaccggggtg
     9781 cagcggatcg tgttcatcgg caaccgcaac ttcggcaacg ctaccctgcg cgaggtcatc
     9841 cagaccaagc agacccggtg gtggcggttc ttctcgacca ccgacagcta cgaccccgac
     9901 cggctgagct tcgaccgcga gctgttgcgg cgcttttacc tgaaggaagg cttcgccgac
     9961 ttccgggtca acagcgccgt cgccgagctc actcccgacc ggtcggcgtt cttcgtcacc
    10021 ttcacggtcg aggaaggcga gcgctatcgg ttcggcgaca tcgacgtggt caacgagctg
    10081 cccgggttcg acccggagac cgtgcgcgat gacgtaacca ccgattccgg ggactggtac
    10141 aacgccgacg aggtcgaaga gacggtccag gacctgaccg agcggatcgg cgcgctgggg
    10201 tacgcgttcg tcgacgtcgc gcccgaggtc aggcgccggc gcgaggacct ggcgatcgac
    10261 gtcacctaca agatcgccga gggcgagcgc atctacgtgc agcgcatcga catcaccggc
    10321 aacgtccgca ccctcgaccg catcatccgc cgcaacatgc ggctggcgga gggcgatgcc
    10381 ttcaatgccg cccgcatccg ccgctcgcgc accctgatcc agggcctcga cttcttctcg
    10441 cgggtggaca tcaacaaccg gccgggcgat tcgcccgacc agaccatcgt caccgtcgac
    10501 gtggaagagc agtctaccgg agaggtgacg ttcggcggcg gcttctcctc gaccgaaggc
    10561 ccgaccggct cgatcggggt tcgcgaacgc aacctgcttg ggcgcggcca ggacctgtcg
    10621 ctgcagttcc tgatctcggg cgtgacccag gacctggacc tcagcttcac cgagccgtac
    10681 ttcctcaatc gcgacttcgc cgcgggcatc gacgtgttcc gtcgcgagcg cgagtttcag
    10741 aacgcctcgt tcgaccgccg cgacctgggc ttttcgctgc gcggcaccta tccgttgtcg
    10801 gagtacctgt cgcaaacggt gcgctacacg ctggcccagg aggagatcct gccctttttc
    10861 ggggcatcga acgtggtcca ggagcaggcc ggggagcggc tcatatcgcg cgtcgaccag
    10921 tccctgctct atgaccgccg cgacaaccgc atcgaaccca ccgacggcta tttcgggcgc
    10981 tggtcggtcg gcattgccgg gctgggcggc gacaagcatt tcctgaagca gtccgtcgac
    11041 gccggctact actactcgtt ttgggacgac tgggtggcgt cgacgctggc ccaggccggc
    11101 tacgtattct cgctgcagag cgacgagaaa gtggacatct cgaaccgttt ctttctcggc
    11161 ggaaacaact tccgcggctt cgcgcccgcc ggcgtcggcc cgcgcgagct gagcgacaat
    11221 accgcactgg gcggcaagat cacctacacc ggcaccctcg aggtggcgtt cccgatcggg
    11281 ctgcccaacg agctcggcgt caagggccgg ctgttcacca tcgccggcag cctgtcggaa
    11341 ctggacaacg agacgccgac cacgatcgat accggctcgt tgcgcatgtc ggtcgggttc
    11401 ggggtatcct gggcgtcgcc gttcggcccg gtccgggtcg acctcgcgac cgccgtggtg
    11461 aaggaggact tcgacgagac cgagctaatc agtttcggct tcgggtcgtt cttctgagtc
    11521 ggcaagcagc gccggtgagg ccagggcacg ccgggttcga aagggtgggg ttcgcatgag
    11581 ccgattgcgg cgggcagcgt tcgggattct gggatttgcg ctgctgttcg cggtgcagtc
    11641 cgcacggacc gagggtcggg cccaggatga gggtgtgatc gcgatcggcg tggtcgattt
    11701 gcagaagatc atgcgcgagt cggtggcggc gcaaaaggtg aggagcgaga tcgaggcgaa
    11761 gcagaccgcc taccgggagc agatcagcgc gcgcgagaac gagctgcgca cccagcagca
    11821 ggagctcgag cgccagcgca ccatactttc cgccgaggcg ttcgccgagc gcgagaagga
    11881 gttctacaag aaggtcgagg ggctgcagcg cgacgtcgcc gagcgcaaca agcagctgga
    11941 ggagtcgctc gcctatgccc gcgatcaggt gcagggtacg gccttgagga tcatcgccga
    12001 gatcgccgac cagaggaagc tcggcctggt gctcgacaag tcgcagctgc tgctggtggc
    12061 gaaaggcctt gaattctcgg accaggcgct ggagcgactc aacgccgaac tgccgtcggt
    12121 ttcgaccacg cccccgccga gagaggacta gtcccggact ccgaatgccc gcgcgcccgc
    12181 gcccacgccc gaccgagccc gatgaccgcg ccgcgccgcg ccggcgaaag gcgagttccg
    12241 cgtgccggcg ttgcccgcgg aggcagtgat acgcggccat ggccgaccct cgcttctatc
    12301 gatcgagggg accgttcgcg ctcggcgagc tcgcgcagag catcggggcg acgctggcgc
    12361 cgggagcgga cgcggacctg aagctcgccg acgtcgctgc gctggacgag gccggtcctg
    12421 agcatctgag cttcttcgac aaccgcaaat atctccagtc gttgcgacag acgcgcgcgg
    12481 gcgcggtggt gctcgccgaa agcgcgcgcg gccaggcgcc gtccggcgcc gcgctgctct
    12541 tctccgctca gccctattac gcctttgcgc ttgcggcgcg cgcattccat ccgatcgtgg
    12601 cggtgagccg cgccggcatc tcgccggcca gccacctcga tccgagcgcg cggctgggcg
    12661 agggttgcca agtcgatgcc ggcgcggtga tcgccgccgg cgcctcgatc ggcagccggt
    12721 gccggatccg ggccaatgcg gcgatcggcg agaaggtggt gatcggcgac gactgcgaca
    12781 tcggcgccgg cgcctcgctc agccactgcc tgatcggcga ccgggtgatc ctgcaccccg
    12841 gcgtctgcat cggtcaggac ggcttcggct tcgcccgtgg cggcgaaggt cacctgaagg
    12901 taccgcagct tggccgggtg atcgtcgaga acgacgtcga gatcggcgcc aacagctcga
    12961 ttgatcgggg cagcgggcct gatactatca tcggcccggg gtgcaagatc gacaacttgg
    13021 tccagatcgc acacaacgtg cgccttggcg ccggatgcgt gatcgtggcg caggtcggaa
    13081 tctcaggcag tacgcagctc ggcgactacg ccgtcctcgc cggccaggtg ggcatcgccg
    13141 gccatctgaa gatcggcaag gaagcggtgc tggcggcgaa atcgggtgtc cacagcgacg
    13201 tgcccgcggg cgcgacctat ggcgggattc cggcccgtcc gatccgccaa tggcggcgca
    13261 tggtcgcggc gatggcccgg ttggggagga acggccgagg ggagggcggc tgaatggcca
    13321 aggcggcggc ggtcgcgggg gcgagcctga tcgacgtgcg gcggatcatg gaaatgatcc
    13381 cccaccgcta tccgttcctg ctggtcgacc gcatcgtcga ggtggtcgag ggggtcagcg
    13441 caaccggggt caagaacgtc accttcaacg agccccactt caccggccac tttccgggca
    13501 cgccggtcat gcccggggtg ctgatcatcg aatcgatggc ccagaccgcg gcgctgttcg
    13561 tggtccacac cctcggcccc gacgccgagg gcaagctggt ctatttcatg accatcgacg
    13621 gcgcccgctt ccgcaagccg gtggtgcccg gcgacaccat gacgatccgg gtcgagaagc
    13681 tgcaggcccg gcgcaacgtc tggaagttcg cctgcaaggt caaggtcggg gatatattgg
    13741 ttgcggaagc gaccatagcg gcgatgatca tggacatgtg atgccgatgc ccgcagacca
    13801 gatcgagccc cggattcacc cgaccgcgat catcgccgcc ggcgccgaga tcggcgccgg
    13861 ggtggccgtc gggccctatt cctgcatcgg tccgcacgtc gagctggacg acggggtcga
    13921 cgtgcatgcg cacgtggtca tcgaaggcca tacccaggtc ggcgcaggca ccagcatctt
    13981 tcccttcgcc tcgatcggcc accgcccgca ggacctgaag taccggggcg agccgtcgag
    14041 cctggtgatc gggcgcaaca ccgtgatccg cgaatacgtc accatgcacc ccggtaccga
    14101 gggcggcggc atggtgacgc ggatcggcga taactgcctg ttcatggtcg cggcgcatgt
    14161 cgcgcatgac tgcaagatcg gcaacggcgt ggtcctggtc aacaacgcca ccctcggcgg
    14221 ccatgtcgcg atcgacgacc acgccatcct cggcggcctc agcgcggtgc accagttcgt
    14281 gcgcatcggc aagcatgcca tggtcggcgg catgagcggg gtcgagggcg acgtcatccc
    14341 ctacggctcg gtggtcggca accgggcgaa cctgtcgggc ttgaacatca tcgggctcaa
    14401 gcgccgcggc ttctcgcgcg atgtcatcca cgatttgcgc aaggcctacc ggctgctgtt
    14461 cgccgaggaa ggcaccatgg ccgagcgcct ggaggacgtg gccgaggaat tcgggcgggt
    14521 cgagccggtg atggagatcg tcgccttcat ccgctccgac agctcgcgcg cgatctgcca
    14581 gcccaagctc gaccgtgccg cctgacccgg cgagcctgcc ggtggcgccg gcggcgggcc
    14641 gcctgggggt gctcgccggc ggcggccggc tgccgcgggt ggtgctcgag gcgtgccggc
    14701 ggcaggggcg cgaggtgttc gcggtcgcct tcgagggcag taccgacccc gagaccgtca
    14761 ccgacatgcc gcaccaatgg gtcaggctcg gcgcggtcgg ccagacgatc gcgctgttga
    14821 aggccaaccg ggtcgaggag ctggtcatgg ccgggccggt ggcacggccg tcctgggccg
    14881 gcatgcgccc cgatctgcgc gcgctcaagc tgctgcccaa ggtgctcgcc ggcggccagg
    14941 gcgacgattc gatcctgtcg cttgccatcc gcgagctcga ggcggaaggc ttccgcgtgg
    15001 tcggcgcgca cgcggtcgtg cccgagctgc tcgcacctgc ggggccgctc ggcgcgcgcg
    15061 tgcccgaccg cggcgcacgc gccgacatcg cgcgcggcgt cgaggtggcg atggcgctcg
    15121 gcgcggtcga tgtcggccag gcggtggtgg tccagcaggg cgtggtgctc ggcatcgagg
    15181 cggtcgaggg caccgacgcc ctgctcggcc gcgccgccac cttgcgccgc gacgggccgg
    15241 gcggggtgct ggtcaagtgc cgcaagccga gccaggaccg ccgcgccgac ctgccctcga
    15301 tcggcgccga cacggtgcgg cgcgcgcgcg aggccggcct gcgcgggatc gcggtcgaag
    15361 cgggcgagac gctggtgctc gaccgggcgc tcgtgatcga ggccgcggac gccggcggcc
    15421 tgttcgtcta cggcttcgaa ggcgggcccg gctgaggccc gccggcggcg acagtctcgg
    15481 gcacaaacag ttgacccagc cctggcccga gctgccctac gccgcgtggc gggacacgct
    15541 cgagacgctg cacctgtgga cccagatcgt cggcaagtat cgcctcgcgc acacgccgtg
    15601 gctcaaccac tcgtggcacg cgacgttcta tgtttccgcc cgcggcctgt ccacgtcgac
    15661 gattccgatg ggcgagcgcg cgctcgagct cgagttcgac ttcatcgacc atgcgctggt
    15721 cgcgcgggcc agcgacggtg cccgcgccga gattggcctg gcaccgatct cggtcgccga
    15781 ctttcacgcg cgtttcgtcg ccatgctgcg tcagctcgac gccgacacct tccttcatgg
    15841 cgtgcccaac gagatcgctg atcctatccc gttcgatcag gatacgcgcc acgcctccta
    15901 cgacgcgggc gcggcgcacc gtttctggca gtcgctggtg cgcatcgatc gggtgttcag
    15961 gctgtttcgc accggctttc tcggcaagag cagcccggtc catttcttct ggggcagctt
    16021 cgacctggcg gtgacgcggt tttcgggccg ccctgcgccc gagcatcccg gcggcattcc
    16081 ggcgcttccc gacccgatca cgcgcgaggc ctactcccac gaggtcagct cggcgggctt
    16141 ctggccgggc ggcggcgcaa tcgactttgc cgcgttctac gcctatgcct atccggctcc
    16201 cgatggcttc gctgccgccg acgtggcgcc ggccgaggcg tactttgatg ccgcactggg
    16261 cgagttcctg ttgccctacg acgccgtgcg cggctcggat cgaccggacc gcgcactcct
    16321 gacgtttcta caggccacct acgccgccgc cgccgatctc ggcaactggg atcgcgcggc
    16381 cctcgactgt cccttgggcg agccgcgcgt cccgcgcccg ttgtcccgct gagcaaaggc
    16441 gggcacgagc gggggcccgg cacgcggtcc cgatcgtgcg cgcggcgaac acccccttgc
    16501 gcgccgggcg cccgccgccc agactcgccc gcgcccgcaa tcgagacgga gtgagagacg
    16561 gacaatggcg gagaccgacg gctttcgcat gcttcatacc atggtccggg tcaaggacct
    16621 ggacgcctcg ctcgacttct acacccgcct gctcggcatg cggctgctgc gcaggcgcga
    16681 ctacccggac gggaagttca cccttgcctt cgtcggctac gggcccgagg agagcagcgc
    16741 cgtgctcgag ctgacctaca actgggacca ggagaagccg tacgacctgg gctcgggcta
    16801 cggccacgtc gcgatcggcg tgcccgacat ctacggcacc tgcgatcggc tgaaagaggc
    16861 cggcgccaag gtggtgcgcg caccggggcc gatgaagcac ggcagcacgg tgatcgcgtt
    16921 cgtcgaggat cccgacggct acaagatcga gctgatcgag cgcg
//
LOCUS       DASWHU010000101         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_409393, whole genome shotgun sequence.
ACCESSION   DASWHU010000101 DASWHU010000000
VERSION     DASWHU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409393"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..822
                     /locus_tag="VGB88_03580"
     CDS             73..822
                     /locus_tag="VGB88_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9646552.1"
                     /translation="MKVLVAVKRVIDFNVKVRVKSDRSGVETANVKMSMNPFDEIAVE
                     EAVRLKEAGKADEIVAVSMGPQQCQETLRTAFAMGADRGILVVSEAELQPLAVAKLLK
                     AICQAETPDLVIMGKQAIDDDSNQTGQMLAALLGWGQGTFASALTPGDGKVEVTREVD
                     GGLETVSLALPAVVTTDLRLNEPRYASLPNIMKAKKKPIDQKTPADFGIDVSPRLTTL
                     KVEEPAKRAGGVKVADAAALVAKLRDEAGVI"
     gene            822..1763
                     /locus_tag="VGB88_03585"
     CDS             822..1763
                     /locus_tag="VGB88_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9646553.1"
                     /translation="MAALVVAEHDNAELKPATLNTVAAAAEIGSEIDLLVAGHGCGAV
                     AEAAAGIAGLRKVLVADAPALAYHLAENMAPLIVRLATDYGHVLVPAGTFGKNFMPRA
                     AALLDVQQISDIVAVEAADTFKRPIYAGNAIATVRSSDAIKLITVRATAFKAAATGAG
                     PAPIEALDGGGDAGLSSFVGRELSKSERPELTAARVIVSGGRGMGSGENFKMLEALAD
                     RLGAAVGASRAAVDAGFVPNDYQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDAEAPIFQVADYGIVGDLFKVVPELTDALAKTK"
     gene            1800..2675
                     /locus_tag="VGB88_03590"
     CDS             1800..2675
                     /locus_tag="VGB88_03590"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9646554.1"
                     /translation="MVDRIAVVGAGQMGSGIAHVCALAGIEVALIDVAEAQLDKALAT
                     IGRNLDRQVARGKIAEADKRAALKRITTAASLAAAASADLVIEAASEDEAVKKAIFKE
                     LCPRLERADALIATNTSSISITRLAAATDRPGRFIGMHFMNPVPVMQLVELIRGIATE
                     EETFAVVRDLAVRLGKTPVSAEDFPAFIVNRILLPMINEAVYTLYEGVGSVEAIDTAM
                     RLGANHPMGPLELADFIGLDVCLAVMQVLHEGLADTKYRPCPLLVKYVEAGWIGRKAG
                     RGFYDYSGDKPVPTR"
BASE COUNT          447 a          915 c          954 g          382 t
ORIGIN      
        1 gcacaatggc ttggtcccgc cgccgtgccc gcggcgcccg atcacggcag tgcgaatccg
       61 gggacggcgc tgatgaaggt tctggtcgcg gtcaagcggg tcatcgactt caacgtcaaa
      121 gtgagggtca agagcgaccg ctcgggagtc gagaccgcca acgtcaagat gtcgatgaac
      181 ccgttcgacg agatcgcggt cgaggaggcg gtgcgcctga aggaagcggg caaggccgac
      241 gagatcgtcg ccgtctcgat gggcccccag cagtgccagg agaccttgcg caccgccttc
      301 gccatgggcg ccgaccgcgg catcctggtg gtctccgagg ccgagcttca gccgctggcg
      361 gtggccaagc tgctcaaggc gatctgccag gccgagacgc ccgacctggt gatcatgggc
      421 aagcaggcga tcgacgacga ttccaaccag accgggcaga tgctggcggc gctgctcggc
      481 tggggccagg gcaccttcgc ctcggcgctg acgccgggcg acggcaaggt cgaggtgacg
      541 cgcgaggtcg acggcgggct cgagaccgtt tcgctcgcgc tgccggcggt ggtgaccacg
      601 gatctccggc tgaacgagcc gcgctacgcc tcgctgccca acatcatgaa ggcaaagaag
      661 aagccgatcg accagaaaac gcccgccgac ttcggcatcg acgtgagccc gcggctgaca
      721 acgctcaagg tcgaggagcc ggcgaagcgc gcgggcggtg tcaaggtggc ggacgcggcc
      781 gcgctggtcg ccaagctccg cgatgaggcg ggggtgatct gatggccgcg ctcgtcgtcg
      841 ccgagcacga caacgccgaa ctcaagcccg cgaccctgaa cacggtcgcc gccgcggccg
      901 agatcgggag tgagatcgac ctgctggtcg ccgggcacgg ctgcggcgcg gtggccgagg
      961 ccgccgccgg catcgccggg ctgcgcaagg tactggtcgc ggatgcgccc gcgctcgcct
     1021 accatctggc cgagaacatg gcgccgctga tcgttcgcct ggcaacggat tatggccacg
     1081 tgctggtccc ggccggcacg ttcggcaaga acttcatgcc gcgggcggcg gcgctgctcg
     1141 acgtccagca gatctcggac atcgtcgcgg tcgaggcggc ggataccttc aagcgcccga
     1201 tctacgccgg caacgccatc gccaccgtgc gctcgagcga cgcgatcaag ctgatcacgg
     1261 tgcgcgcgac cgcgttcaag gcggcggcga cgggcgccgg gccggcgccg atcgaggcgc
     1321 tcgacggcgg cggcgacgcc gggctgtcgt cgttcgtcgg ccgcgagctg tccaagtccg
     1381 agcggccgga gctcaccgcc gcgcgggtga tcgtctccgg cggccgcggc atgggctcgg
     1441 gcgagaactt caagatgctg gaggcgctcg ccgaccgcct cggcgccgcg gtcggcgcct
     1501 cgcgcgcggc ggtcgacgcg ggcttcgtgc ccaacgacta ccaggtcggc cagaccggca
     1561 agatcgtcgc ccccgacctc tacatcgcgg tcggcatctc gggcgcgatc cagcacctcg
     1621 ccgggatgaa ggactccaag gtcatcgtcg cgatcaacaa ggacgcggag gcgccgatct
     1681 tccaggtcgc cgattacggc atcgtcggcg acctgttcaa ggtggtgccg gagttgaccg
     1741 acgccctggc caagaccaag tagcagcgga cgggcgcctg cgggggaaac cgggaaatca
     1801 tggtcgacag gatagcggtc gtcggcgccg gccagatggg ctctggcatc gcccatgtct
     1861 gtgcccttgc cggcatcgag gtggccttga tcgatgtcgc cgaggcgcag ctcgacaagg
     1921 cgctcgccac catcggccgc aacttggacc gccaggtcgc gcgcggcaag atcgccgagg
     1981 ccgacaagag ggcggcgctg aagcgcataa ccacggccgc aagcctcgcc gccgcggcgt
     2041 ccgccgacct cgtgatcgag gcggcgagcg aggacgaggc ggtcaagaag gcgatcttca
     2101 aggagctgtg cccgaggctc gagcgcgcgg atgcgctgat cgcgaccaac acctcttcga
     2161 tctcgatcac ccgcctcgcc gccgccaccg accgcccggg ccggttcatc ggcatgcact
     2221 tcatgaaccc ggtgccggtg atgcagctgg tcgagctgat ccgcggcatc gccaccgagg
     2281 aagagacgtt tgccgtggtc cgcgatcttg ccgtccgcct cggcaagacg ccggtcagcg
     2341 ccgaggactt ccccgccttc atcgtcaacc gcatccttct gccgatgatc aacgaggcgg
     2401 tgtacacctt gtatgaaggc gtcggctcgg tcgaggcgat cgacaccgcg atgaggctcg
     2461 gcgccaacca cccgatgggg ccgctcgagc tcgccgactt catcggcctc gacgtgtgcc
     2521 tggcggtgat gcaggtgctc cacgagggcc tcgccgacac caagtaccgt ccctgcccgc
     2581 tgctggttaa gtatgtcgag gccggctgga tcgggcgcaa ggccgggcgc ggcttctacg
     2641 actactcggg cgacaagccg gtgccgacgc gctgacacgg tcgcaaggag accgttgc
//
LOCUS       DASWHU010000102         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2626679, whole genome shotgun sequence.
ACCESSION   DASWHU010000102 DASWHU010000000
VERSION     DASWHU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626679"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGB88_03595"
     CDS             complement(<1..230)
                     /locus_tag="VGB88_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9646555.1"
                     /translation="MTFPDVPEAITFGSDRENALANAVDALSTALAEYARRGLAAPRP
                     SRPGPGQAMIAADAVIALKLALAEAMREQRIK"
     gene            complement(461..619)
                     /locus_tag="VGB88_03600"
     CDS             complement(461..619)
                     /locus_tag="VGB88_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9646556.1"
                     /translation="MPVSVRQRRGKGSHLLLDFGDRWTTIPNHPGDLPPGTLRAIRRQ
                     LGIDPDEL"
     gene            892..1290
                     /locus_tag="VGB88_03605"
     CDS             892..1290
                     /locus_tag="VGB88_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9646557.1"
                     /translation="MSGSEAQGEPGPEAAYFAHLARGEFAIQHCDGCARWVFYPRIAC
                     PHCGGRALTFQRPSGRGVVYATSVVRPRPEQGAPYNVALIDLDEGPRMMGRIEGVAPE
                     AVGIGLKVVAEVDTGGATPVVVFRAAKAER"
     gene            1287..2438
                     /locus_tag="VGB88_03610"
     CDS             1287..2438
                     /locus_tag="VGB88_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX9646558.1"
                     /translation="MSAALRGEVAIAGAATHALGEAPGRSEMEITAGAALAALDDAGL
                     KPGDVDGLFCTLMRHNMASLSLGEYLGIRPALTDSSMIGGSSNLAHLILAQLAIHAGL
                     CRVALIAYGSNQRSAAGRLVSTSVPNPYEAPYRPRNPITAYALAASRHMHQYGTTRIQ
                     LAEVAVAARAWAALNDEAFMRAPLSVDEVLSAHPVSDPFTVRDCCLVTDGGGAVVLVA
                     AERARDLRKPPVAVLGAGLAHWHRDISNMPDLTVTAATESGARAYAMAGIQAGDVDVV
                     ELYDAFTINPIVFLEDLGFCPKGDGGRFVADGAIAPGGRLPVNTNGGGLSCCHPGMYG
                     IFPLIEAVRQLRGEAGARQVQGAEIALAHGNGGLFSSQVTVVLGTAETV"
     gene            complement(2788..>3634)
                     /locus_tag="VGB88_03615"
     CDS             complement(2788..>3634)
                     /locus_tag="VGB88_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9646559.1"
                     /translation="LMADLQLGLHIFGRYGVTIFALSGLDIALWDIAGKAAGAPLHRL
                     LGGLARESVPAYASLLKYQDAAVVAELTTEALGKGYRLVKLHEVSAREVAAARAAAGD
                     RVPIMLDVNCVWSPMRAAAMAEELFDYDLHWLEEPIWPPENYDALAELRAACGMRLAA
                     GENACTAWQFRAMIEAGAVDYAQPSVTKVGGISEFVKIAALAETGNVALAPHSPYFGP
                     GLLATLQLLAAYPAIESIERFYVDLDADLYDGALTPKDGEIAVPTGPGLGLDPNPDFI
                     ARYRS"
BASE COUNT          529 a         1268 c         1252 g          585 t
ORIGIN      
        1 ttcttgatcc gttgctcccg catcgcttcg gccagcgcca gtttcagcgc gatcacggca
       61 tcagccgcga tcatcgcctg gcccgggccg gggcgcgacg ggcgcggcgc agccaagccg
      121 cgccgagcgt attccgcgag cgccgtgctc aaggcgtcga ccgcgttggc aagcgcgttt
      181 tcgcggtccg agccgaaggt gatcgcttcc ggaacgtcgg gaaaggtcac cgtataacgc
      241 ccgtccgctt cctcactcag tcgtgcggga tacacgaaag tccatgctgc tgttgccatg
      301 gcactcttga ttgacttggt tctgacgttc gcacttgggc cgctgatctc agttctcccg
      361 cgccgtttgg tcctcgcggt tcatacagat cactacattc tctcgatgcg ttcagagatt
      421 tcggcttgtc aatccgcccg gcaaccgacc tgggactgcg ctacagctcg tcaggatcga
      481 tgccgagctg tcggcggatc gcccgcaagg tgcctggtgg caggtcgccg gggtggttcg
      541 ggatcgttgt ccagcggtcg ccgaagtcga gcagcaagtg gctgcccttt cctctccgtt
      601 gccggaccga aaccggcatc ccccgctttc gccccagtcg ccgaatcctg cgaacaagct
      661 cgcttgcggt catcgcgttc cgacatgctt taatgggcat tcttgtccgg acagtcaagg
      721 acaattcgga cacgaatgac cggagccacg gaaatcctat ccctgcgagt caaagcttcc
      781 cggtggttct tcgatagcct tcacggctcg caattggttc gtccgtccct ataatcgcgc
      841 gccttgccgg gcgagtgccc ggcgccaggg cgacaaggcg gggagggacg gatgagcggc
      901 agcgaagctc agggtgagcc ggggccggaa gcggcttact tcgcgcacct cgcgcgcggc
      961 gagttcgcca tccagcactg cgacggctgc gcgcgctggg tgttctaccc gcgcatcgcc
     1021 tgcccgcact gcggcgggcg cgcgctgact ttccaacgcc cgtcggggcg cggggtggtc
     1081 tatgccacca gcgtcgtccg cccgcggccc gagcagggcg cgccgtacaa cgtcgccctg
     1141 atcgacctgg acgagggccc gcgcatgatg gggcggatcg aaggcgtggc gccggaggcg
     1201 gtgggcatcg gcctcaaggt ggtcgccgag gtcgataccg gcggcgcgac gccggtggtg
     1261 gtgttccgcg ccgcgaaggc agagcggtga gcgcggcctt gcgcggcgag gtcgccatcg
     1321 ccggggcggc cacccacgcg ctcggcgagg caccggggcg ctcggagatg gagatcaccg
     1381 ccggcgcggc gctggcggcg ctcgacgacg ccggcctcaa gcccggcgac gtggacggcc
     1441 tgttctgcac cctgatgcgc cacaacatgg cctcgctcag cctcggcgag tatctcggca
     1501 tccgcccggc gctgaccgac tcctcgatga tcggcggctc gtcgaacctc gcccacctga
     1561 tcctcgccca gctcgccatc cacgccggcc tgtgccgggt ggcgctgatc gcctacggca
     1621 gcaaccagcg cagcgcggcc gggcgcctgg tgtcgacctc ggtccccaat ccgtacgagg
     1681 cgccctaccg gccgcgcaac ccgatcacgg cttacgcgct ggccgcgtcg cggcacatgc
     1741 accagtacgg caccacccgc atccagctcg ccgaggtggc ggtggcggca cgcgcctggg
     1801 cggcgctcaa cgacgaggcg ttcatgcgcg ctccgctcag cgtcgacgag gtgctgtcgg
     1861 cgcatccggt gagcgacccg ttcacggtcc gcgactgctg cctggtcacc gacggcggcg
     1921 gcgcggtggt gctggttgcg gccgagcgcg cgcgcgactt gcgcaagcct ccggtcgcgg
     1981 tgctcggcgc ggggctcgcc cactggcacc gcgacatctc caacatgccc gacctgacgg
     2041 tgaccgcggc gaccgagtcg ggcgcgcgcg cctacgccat ggccggcatc caggccggcg
     2101 acgtcgacgt ggtcgaattg tatgacgcct tcaccatcaa cccgatcgtg tttctcgagg
     2161 acctcggctt ctgtcccaag ggagacggcg ggcggttcgt cgccgacggc gcgatcgcgc
     2221 ccggcggccg gctgccggtc aacaccaacg gcggcgggct gtcgtgctgc catccgggca
     2281 tgtacggcat cttcccgctg atcgaggcgg tccgccagct gcgcggcgag gccggcgcgc
     2341 gccaggtgca gggcgccgag atcgcgctgg cacacggcaa cggcgggctg ttctcgagcc
     2401 aggtcacggt cgtgctcggc accgccgaga cggtgtagcg gactggtgcc ggcggccctg
     2461 gcgggaattt gacacgaacg ccccgccacc atcttgaatc atcggcgggg gtgcttatgt
     2521 cagcgttaat ctggccagat tgcgcagcta ctgcgcggcg cccctgagct cagcccccca
     2581 ctcgctcagg ggcgcttttc atttgccggc cgccttgcta tctagccagc cggcgccgac
     2641 agcgggccca gcaagcgggg acgggcatgg ccggagacaa gacgcacagg gattacagcc
     2701 tggtctgggt gacgatcggc acgatcgcgt tcgccgcgat cgccatctac atcatccaga
     2761 gcgtgctgcc ttgagcctgt cgccggccta gctcctgtac cgcgcgatga agtccgggtt
     2821 cgggtcgagg ccgagcccgg ggccggtcgg caccgcgatc tcgccgtcct tcggcgtgag
     2881 cgcgccgtcg tagaggtcgg cgtcgaggtc gacgtagaag cgctcgatcg actcgatggc
     2941 cggataggcg gcgagcagct gcagggtcgc gagcaagccc ggcccgaaat agggcgagtg
     3001 cggggcgagc gcgacgttgc cggtctcggc cagggccgcg atcttgacga actcgctgat
     3061 ccctccgacc ttggtcacgc tcggctgggc atagtcgacc gcgccggcct cgatcatcgc
     3121 ccggaactgc caggcggtgc aggcgttctc accggcggca aggcgcatgc cgcaggccgc
     3181 gcgcagctcg gccagcgcgt cgtagttctc gggcggccag atcggctcct cgagccagtg
     3241 caggtcgtag tcaaacagct cctcggccat cgccgcggcc cgcatcgggc tccacacgca
     3301 gttgacgtcg agcatgatcg gcacgcgatc gccggccgcc gcgcgcgccg cggccacctc
     3361 gcgcgcgctt acctcgtgca gcttgaccag gcgatagccc ttgccgagcg cctccgtggt
     3421 gagctccgcg acgaccgccg catcctggta tttgagcagg ctcgcatagg ccggcacgga
     3481 ctcgcgggca agcccgccga gcaggcggtg gagcggtgcg ccggccgcct tgccggcgat
     3541 gtcccacaac gcgatgtcga gcccgctcaa ggcgaagatg gtgacgccgt aacggccgaa
     3601 gatgtgcagc ccgagctgca ggtcggccat caag
//
LOCUS       DASWHU010000103        13378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2627900, whole genome shotgun sequence.
ACCESSION   DASWHU010000103 DASWHU010000000
VERSION     DASWHU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627900"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGB88_03620"
     CDS             <1..438
                     /locus_tag="VGB88_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9646560.1"
                     /translation="VLSRQIAELGIYPAVDPLDSTSRMLDPRVVGEHHYKVARRVQEI
                     LQRYKSLQDIIAILGMDELSEEDKLTVARARKIQRFLSQPFHVAEVFTGTPGTLVSLE
                     DTIAGFEAICAGEGDDMPEASFYMVGAFDQARDKARQMAAEAA"
     gene            449..877
                     /gene="atpC"
                     /locus_tag="VGB88_03625"
     CDS             449..877
                     /gene="atpC"
                     /locus_tag="VGB88_03625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9646561.1"
                     /translation="MAEADGKVAFELVSPERLLISKPVEMVVVPGDEGDFGVLPGHAP
                     LISELRSWVLEVYDAGQVSERVFVAGGFAEVTAERCTVLAEEAVALAEASAADAEARL
                     KRAAQAVDDARDEDERAPAEAERARAQALVEALAYYGAGR"
     gene            1032..1844
                     /locus_tag="VGB88_03630"
     CDS             1032..1844
                     /locus_tag="VGB88_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9646562.1"
                     /translation="MKGWTLDDVPWQRFAPEKVDPGLLAAVKAAALVEHNGRDYETYL
                     HHVFADDPEFRAAVSTWAQEEVRHGLALAAWAKRADPGFDFEARFRRFRDGYRLPLDA
                     TSSVRGSRSGELIARCVVEAGTSSFYTALSTASDEPVLKEICRLIAADEFRHYKLFYD
                     TLGRYLKSERIGRARRLWVAVGRVLESEDDELSFAYYCGNDLAGPYRRKDCARAYERR
                     VGPVYRPGHLARGVAMVLKAGGVRPQGWVGRNLSRLAWYAFRWRSATPAAAG"
     gene            complement(1851..2369)
                     /locus_tag="VGB88_03635"
     CDS             complement(1851..2369)
                     /locus_tag="VGB88_03635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX9646563.1"
                     /translation="MSEPAPPTDALPYRPCVGIMLLDRHGRAFVGQRADQPGDAWQMP
                     QGGIDIERGEAPRAAALRELSEEIGIEAVEVLAEGRGWHRYDLPEALAGRAWGGRFRG
                     QEQKWFAMRFLGGDEDIDLGAHEREFSRWRWVEPERLPALVVPFKRAVYEAVVAEFRP
                     LFERLRAAPAGG"
     gene            complement(2366..3334)
                     /locus_tag="VGB88_03640"
     CDS             complement(2366..3334)
                     /locus_tag="VGB88_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX9646564.1"
                     /translation="MAAIGGLLGWLASQPPLPDPLPYYVAVPRADAPAALGAADPDAP
                     ISVTPLAEPEVAEATASPEAAPVEDGRYGPVPVVTGDGRQSWQVYARPYDASDARARI
                     AVVVVGLGRGQADTNKALELPGEVTLAFSPYGEALDDWARQARAAGHEVLLEVPMEPR
                     NFPRDDPGPRALLTSLGPNQNLDRLEWLLARFTGYVGVVDLAGDRFTASEPHLHPLLD
                     ELKRRGLVYLDRFGAGSLAERIAGDVGLMRLSADLVLDDEPSRAEIGRRLVAAEAYAR
                     RAGSVVLIARTYPVTFDMLQRWFTELDGDALALAPLTAALARRAEG"
     gene            complement(3483..4826)
                     /locus_tag="VGB88_03645"
     CDS             complement(3483..4826)
                     /locus_tag="VGB88_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9646565.1"
                     /translation="MAALAGLALAVTVPNVEAASSETYRQLNLFGDVFERVRAEYVEE
                     VSDEKLIEAAINGMLHDLDPHSSYLPPRDFKDMQVQTRGEFGGLGIEVTMENGLVKVV
                     SPIDDTPAFRAGMQPGDLISHIDGEQVLGMTLAEAVEMMRGPIDSKITITVLRESEQA
                     PFDVTLVRALIKIRSVRSRAEGNIGYVRITSFNEQTEPGLEEAIKSLRAELGENMKGV
                     VLDLRNNPGGLLDQAVAVSDAFLEQGEIVSTRGRDPENVQRYNAKAGDLSVGLPMVVL
                     INGGSASASEIVAGALQDHRRAILLGTKSFGKGSVQTIVPLPGNGAMRLTTARYFTPS
                     GRSIQATGIDPDIVVERTTIEEVAAAKQSREADLPKALDTDKGGQALPESDVAPAPAP
                     EPEPEPEPGAEVEPGPEAEPGPEAEKAEGEPELIDYQLRRALDLLEGIALFDKQA"
     gene            complement(4841..6184)
                     /locus_tag="VGB88_03650"
     CDS             complement(4841..6184)
                     /locus_tag="VGB88_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9646566.1"
                     /translation="MPPSSARAAPILAAAALAAHAVCASAAGVTSGDPQARLDVIESS
                     LAARRAEQSALERRAGDLAAEIDELREQLVDAAAAVQDFELRLHRQEDDLALLEARIE
                     EARGDLALTRGGLAATLGALVLIARHPPEALLVRPGKWSDTVHGVRLLEQSATEFGER
                     TIGLAEDLAALRALRAEQTSRQAALVELRTDVADRRAVLRQLLERKSSLQATLMADRR
                     NLARDIGALGAQARDVRDLIEVIRKARAEISARRSVEHHALAEAAERSAAEVAEADRL
                     NEIEPVASPAITAAVAKPDPPAPRPAEGARRLAALPEVPTGSSLPYPVHGDIVRRFGE
                     ARGAGEPSQGLGIAARAGAEVVAPRAGEVVFAGPFRSYGQLLILEHAGGYHTLLSGFS
                     RLHCEVGQRVRAGEPVGRMAEDAGEATILYVEIRHNSQPIDPVPWLMSGSRKVSG"
     gene            complement(6171..7742)
                     /gene="gpmI"
                     /locus_tag="VGB88_03655"
     CDS             complement(6171..7742)
                     /gene="gpmI"
                     /locus_tag="VGB88_03655"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9646567.1"
                     /translation="MTAFAGTRPVVLCILDGWGVRRAADDNAIELADTPTWDRLWAGC
                     PHARIDASAGEVGLPAGQMGNSEVGHMNIGAGRVVMQDLPRIDAALADGSLARAPALA
                     RLIDRLKATGGACHLLGLMSPGGVHSHQRHIAALARTVAAAGVPVRVHAFLDGRDTPP
                     RSGRDYLARFDADLAGAGGVAVATVGGRYYAMDRDRRWERTGRAYRVLVDAAGEHAQS
                     AVAAVEASYAAGIGDEFVAPVAIAGYGGMADRDGLVMANFRADRVRQILAALLDPEFG
                     GFARARTVAFAAALGMVSYSARLDRFLDVIFPPLALTDTLGEVVARAGLRQLRLAETE
                     KYAHVTYFLNGGREETFDGEERILVPSPKVATYDLKPEMSAFEVTDRLVEAVAAGRHE
                     LIVVNYANTDMVGHTGKLAAAIAAVEAVDACLGRLVEAVTAAGGALLITADHGNAEQM
                     SDRASGQAHTAHTTNPVPVVLVGGPAGARLDHGRLADIAPTVLELLGLARPAAMTGRS
                     LIAGAGQAAAGRAAE"
     gene            complement(7789..8154)
                     /gene="rsfS"
                     /locus_tag="VGB88_03660"
     CDS             complement(7789..8154)
                     /gene="rsfS"
                     /locus_tag="VGB88_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9646568.1"
                     /translation="MTDADQSLRVDALTRIACNSLEEDKAEDVTVIDLAGKTNISDYM
                     IIASGRSQRHVAAIAEHLVQRLKRRGSAPLSVEGLAQADWVLIDAGDVIVHVFRPEVR
                     SFYNLEKMWAVPLPEHAVH"
     gene            complement(8151..8783)
                     /locus_tag="VGB88_03665"
     CDS             complement(8151..8783)
                     /locus_tag="VGB88_03665"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX9646569.1"
                     /translation="MLPPGRRRPRIGLLGGSFNPAHDGHRHVSRRAQALLGLDVVWWL
                     VSPQNPLKPTAGMAPLAARLSRARAVAGHGAIRVTAIERILGTRYTVDTVAALKRRLP
                     QARLVWLMGADNLIQMPRWHRWPALFHALPIAIFDRPTYSHSALLCPATRRFARYRRR
                     LRDPARLVDCPPPAWAWIGGGLHPASATRIRDSGGWGRPDGRMAEGRSET"
     gene            complement(8810..10093)
                     /locus_tag="VGB88_03670"
     CDS             complement(8810..10093)
                     /locus_tag="VGB88_03670"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX9646570.1"
                     /translation="MSLARQTTEGDIHAQMLAIGRAARAAAAQLARVETAAKNAALNA
                     AAGAVRAGANELLAANADDLAAARKSNQTEAFLDRLALDAARIEAMAEGLEDVAGLPD
                     PVGQVIGEWTRPNGLRITRVRVPLGVIGIIYESRPNVTADAGGLCLKSGNAVILRGGS
                     ESFHSSRAIHRCLVHGLKVAGLPEAAIQLVPTRDRAAVGEMLTMTDYIDVVVPRGGKS
                     LVARVAADSRVPVIKHLDGICHVYIDRAADPAMAEEVSFNAKMRRTGICGAAETVLVH
                     RDAAGAVLPRLVRRFLDGGCEVRGDDAVQRIDARVKPATPADWDTEYLDAIISIKTVA
                     DLDEAIAHVERHGSHHTDSIVTADVAAAERFLNEVDSGIVLHNASTQFADGGEFGMGA
                     EIGISTGKLHARGPVGLEQLTSYKYVVHGSGQTRP"
     gene            complement(10090..11229)
                     /gene="proB"
                     /locus_tag="VGB88_03675"
     CDS             complement(10090..11229)
                     /gene="proB"
                     /locus_tag="VGB88_03675"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX9646571.1"
                     /translation="MTERLAKARRVVIKIGSSLLTEGDDGRLRDSWLAALADDVAAMR
                     ARGQQVALVSSGAIAVGRRRLGLGRRELRLEDKQACAAVGQIRLAHAWQQALDRHGIT
                     VAQLLLTLEDSEDRRRYLNARATLESLLKLGALPVINENDTVATAEIRFGDNDRLAAR
                     VAQMASAEVLILLSDIDGLYTADPRLDASARRIDEIDEITPEVERMAGASLSDSGSGG
                     MITKLAAARNAVRAGCHMVIAEGMAAHPIERLLAGAPCTWFLAHASPLTARKHWIAGT
                     LQPAGALVVDDGAARALGQGNSLLPAGVLAAEGSFRRGDLVLVRDRAGTELGRGLSAY
                     ACADARRILGHKSREIEQILGYRGREEMIHRDDLVLVREADREQK"
     gene            complement(11226..12248)
                     /gene="obgE"
                     /locus_tag="VGB88_03680"
     CDS             complement(11226..12248)
                     /gene="obgE"
                     /locus_tag="VGB88_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9646572.1"
                     /translation="MRFLDQAKIYVKAGDGGNGCVAFRREKRVAYGGPSGGNGGRGGD
                     VVAVAADDLNTLIDFRYRQHFRARPGANGSGSERTGASADVLVIRVPVGTEILAEDKA
                     TVIADMTEPGERRVLAEGGRGGRGNAAFKTSTNQAPREAEPGRAGEERWLWLRLKLIA
                     DVGLVGLPNAGKSTFLAAASRARPKIADYPFTTLTPQLGVARVGEREIVIADIPGLIE
                     GAHAGSGLGDRFLGHIERCAAILHLVDGAGEDVAAAYRTIRGELGAYGHGLAGKPALV
                     GLNKCDLLDAPAERAAERALAAAGAERVLALSAATGEGVPEVIGALMDLVAAARAPAA
                     EEPRQP"
     gene            complement(12370..12627)
                     /gene="rpmA"
                     /locus_tag="VGB88_03685"
     CDS             complement(12370..12627)
                     /gene="rpmA"
                     /locus_tag="VGB88_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9646573.1"
                     /translation="MAHKKAGGSSRNGRDSAGRRLGVKRFGGQIVVPGNILVRQRGTK
                     FHPGDHVGIGTDHTLFALSPGRVQFRHGRLGRTFVSVEPAG"
     gene            complement(12634..12984)
                     /gene="rplU"
                     /locus_tag="VGB88_03690"
     CDS             complement(12634..12984)
                     /gene="rplU"
                     /locus_tag="VGB88_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9646574.1"
                     /translation="MYAVIRTGGKQYRVARNDVIAVEKLAGAAGGEVAFEDVLLVAGE
                     GGVQAGAPTVAGARVTATVLEQALGEKIIVFKKKRRKNYRRTRGHRQAQTVVRIADIT
                     LATAPAAPAAKTEE"
     gene            13189..13276
                     /locus_tag="VGB88_03695"
     tRNA            13189..13276
                     /locus_tag="VGB88_03695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13223..13225,aa:Ser,seq:cga)
BASE COUNT         1735 a         4896 c         4884 g         1863 t
ORIGIN      
        1 gtgctcagcc gccagatcgc cgagctcggc atctacccgg cggtggaccc gctcgattcg
       61 acctcgcgca tgctcgaccc gcgggtggtc ggcgagcacc actacaaggt cgcgcgccgg
      121 gtccaggaga tcttgcagcg ctacaaatcc ctgcaggaca tcatcgccat cctgggcatg
      181 gacgagctgt cggaggagga caagctgacg gtggcgcgcg cgcgcaagat ccagcgcttc
      241 ctgtcgcagc cgttccacgt cgccgaggtg ttcaccggca ccccgggcac cctcgtcagc
      301 ctcgaggaca ccatcgccgg cttcgaggcc atctgcgccg gcgagggcga cgacatgccc
      361 gaggcgtcgt tctacatggt cggcgcgttc gaccaggcgc gcgacaaggc gcgtcaaatg
      421 gcggccgagg ccgcctgagg cgccggccat ggccgaggcc gacggcaagg tcgccttcga
      481 gctggtctcg ccagagcggc tgctgatctc gaagccggtc gagatggtgg tggtgccggg
      541 cgacgagggc gacttcggcg tgctccccgg ccacgcaccc ctgatctcgg agctgcgcag
      601 ctgggtgctg gaggtctacg acgccggcca ggtgagcgag cgggtgttcg tcgccggcgg
      661 cttcgccgag gtcacggccg agcgctgcac cgtgctcgcc gaggaggcgg tcgcgctcgc
      721 cgaggcgagc gcggcggacg cggaggcgcg gctcaagcgc gccgcgcagg cggtggacga
      781 cgcccgcgac gaggatgagc gcgcccccgc cgaggccgag cgcgcccgcg cccaagccct
      841 ggtcgaggcg ctcgcctact acggcgcggg gcgctgagcg cgcggcgcgc ggcccagccc
      901 gcagcggatc gggcgacgcg cagcctgcgg tcaagcgccg ccacggtctt actttgaaat
      961 cgggcggcgt tgtcttatcc ttgcggcaag gaagagggga cagggacgca agggtgattt
     1021 gaggctcggc tatgaaaggt tggacgctgg acgacgttcc atggcagcgc ttcgcgcctg
     1081 agaaggtcga ccccggtctt ctggccgcgg tcaaggcggc ggcgctggtc gagcacaacg
     1141 ggcgcgacta cgagacctat ctgcaccacg tgttcgccga cgaccccgag ttccgcgccg
     1201 cggtcagcac ctgggcgcag gaagaggtcc gccacggcct cgcgctcgcc gcgtgggcga
     1261 agcgcgccga ccccggcttc gacttcgagg cacggttccg gcgcttccgc gacggctatc
     1321 gcctgccgct cgatgccacc agctcggtgc gcggctcgcg gagcggcgag ctgatcgcgc
     1381 gctgcgtggt cgaggcgggg acctcgtcgt tctacaccgc gctcagcacc gcctccgacg
     1441 agccggtgct caaggagatc tgccggctga tcgcggccga cgagttccgc cactacaagc
     1501 tgttctacga caccctcggc cgctacctga agagcgagcg catcggccgc gcacgccggc
     1561 tgtgggtggc ggtcggacgg gtgctcgaaa gcgaggacga cgagctgtcg ttcgcctact
     1621 actgcggcaa cgacctggcc gggccgtacc ggcgcaagga ctgcgcgcgc gcctacgagc
     1681 gccgggtggg cccggtctac cgcccggggc acctggcgcg cggcgtggcg atggtgctca
     1741 aggccggcgg ggtgcgcccg cagggctggg tcgggcgcaa cttgagcagg ctcgcctggt
     1801 atgcgtttcg ctggcgcagc gcgacgccgg cggccgcggg gtgaggccgg tcagccgccc
     1861 gcgggcgcgg cgcgcaggcg ctcgaacagg ggccggaact cggccaccac cgcctcgtag
     1921 accgcgcgct tgaacggcac caccagcgcc ggcagccgct cgggctcgac ccagcgccag
     1981 cgactgaact cgcgctcatg ggcgccgaga tcgatgtcct cgtcgccgcc gaggaagcgc
     2041 atcgcgaacc atttctgctc ctggccccgg aagcggccgc cccaggcccg accggccagc
     2101 gcctcgggca ggtcgtagcg gtgccagccg cggccctcgg cgagcacctc gaccgcctcg
     2161 atgccgatct cctcgctgag ctcgcgcaac gccgccgcgc gcggcgcctc gccccgctcg
     2221 atgtcgatgc cgccctgcgg catctgccag gcgtcgcccg gctggtcggc gcgctggccg
     2281 acgaaggcgc gcccgtgccg gtcgagcagc atgatgccga cgcacggccg atagggcagc
     2341 gcgtcagtcg gcggcgcggg ctcgctcatc cctcggcgcg tcgggccagc gcggcggtca
     2401 gcggcgccag cgccagcgca tcgccgtcga gctcggtgaa ccagcgctgg agcatgtcga
     2461 aggtgaccgg ataggtgcgc gcgatcagca ccacgctgcc ggcgcgccgc gcataggcct
     2521 cggcggcgac cagccggcgc ccgatctcgg cgcgcgacgg ctcgtcgtcg agcaccaggt
     2581 cggcgctcag gcgcatcagg ccgacgtcgc cggcgatgcg ctcggcaagg ctgccggccc
     2641 cgaaacgatc gagataaacc agcccgcggc gcttgagctc gtccagcagc gggtggagat
     2701 ggggctcgga ggcggtgaac ctgtcgcccg ccaggtcgac caccccgaca tagccggtga
     2761 accgggccag cagccactcc agccggtcga ggttctggtt cgggccgagc gaggtcaaga
     2821 gcgcccgcgg tcccgggtcg tcgcggggga agttgcgcgg ctccatcggc acctcgagca
     2881 gcacctcgtg gcccgccgcg cgcgcctggc gcgcccagtc gtcgagcgcc tcgccatagg
     2941 gcgagaacgc gagcgtgacc tcgccgggca gctcgagcgc cttgttggtg tcggcctggc
     3001 cgcggccgag gccgaccacc acgaccgcga tccgggcgcg cgcatcgctc gcgtcgtagg
     3061 ggcgggcata gacctgccag gactggcggc cgtcgcccgt caccaccggc accggcccgt
     3121 agcggccatc ctcgaccggt gcggcctcgg ggctcgcggt tgcctcggca acctcgggct
     3181 cggcgagggg ggtcacggag attggcgcat ccgggtcagc cgcgccgagc gccgccggcg
     3241 cgtccgcgcg cggcacggcg acgtagtagg gcagcgggtc gggcagcggc ggctggctcg
     3301 ccagccagcc gagcaggccg ccgatcgcgg ccaccgacac cgccgcgagc agccccgagg
     3361 cgcgctgccg gccgcggccc gcgcgagctc gccgcggcgc ccgtgccgcc gctaggccca
     3421 ttcccccacg gtaacgcgcc gcgcggcggc ggcaatcggt cgggccgcgg ccggcatccg
     3481 cctcacgcct gcttgtcgaa cagggcgatg ccttcgagca ggtcgagcgc gcgccggagc
     3541 tggtagtcga tcagctcagg ctcgccttcg gctttctccg cctcggggcc gggctccgcc
     3601 tcagggccag gctccacctc cgcacccggt tccggctcgg gctcgggctc gggcgcgggc
     3661 gcgggcgcga catcgctctc cggcagcgcc tgcccgccct tgtcggtgtc gagcgctttc
     3721 ggcaggtcgg cctcgcggct ctgcttggcc gccgccacct cctcgatggt ggtgcgctcg
     3781 acgacgatgt ccggatcgat gccggtcgcc tggatcgacc gccccgaggg cgtgaaatag
     3841 cgcgccgtgg tcagccgcat cgcgccgttg cccggtagcg gcacgatggt ctggaccgag
     3901 cccttgccga acgacttggt gcccagcagg atcgcccggc ggtggtcctg gagcgcgccc
     3961 gcgacgatct cggaggccga ggccgagccg ccgttgatca gcaccaccat cggcaggccg
     4021 acactcaggt cgccggcctt ggcgttgtag cgctggacgt tctccgggtc gcggccgcgg
     4081 gtggacacga tctcgccctg ctcgaggaag gcgtcgctca ccgccaccgc ctgatcgagc
     4141 agcccgccgg gattgttgcg cagatccagc accacgccct tcatgttctc gccgagctcg
     4201 gcgcgcagcg acttgatcgc ctcctcgagc ccgggctcgg tctgctcgtt gaacgaggtg
     4261 atgcgcacat agccgatgtt gccttcggcg cgcgagcgca ccgagcggat cttgatcaag
     4321 gcgcgcacca gggtgacgtc gaacggcgcc tgctcggact cgcgcagcac ggtgatcgtg
     4381 atcttggaat cgatcggccc gcgcatcatc tcgaccgcct cggccagggt catgccgagc
     4441 acctgctcgc cgtcgatatg gctgatcagg tcgcccggct gcatgccggc gcgaaaggcg
     4501 ggcgtgtcgt cgatcggcga gaccaccttg accagcccgt tctccatcgt cacctcgatg
     4561 ccgaggccgc cgaactcgcc gcgggtctgg acctgcatgt ccttgaagtc cctgggcggc
     4621 aggtagctgg agtgcgggtc gagatcgtgc agcatgccgt tgatcgcggc ctcgatcagc
     4681 ttctcgtccg aaacctcctc gacatattcc gcgcgcacgc gctcgaacac gtcgccgaac
     4741 aggttgagct ggcggtaggt ttccgagctt gccgcctcga cgttcgggac ggtcaccgcc
     4801 agcgccagac cggcgagcgc ggccacaaca agattgcgta tcatccactg acctttcgac
     4861 tgcccgacat cagccacggc accggatcga tcggctggct gttgtggcgt atctccacat
     4921 acaagatagt agcctcgccg gcgtcttccg ccatacggcc gacgggttct ccggcccgga
     4981 cacgctgtcc cacctcgcaa tgcaggcggg aaaagccgga tagcaaagta tgatatccgc
     5041 ctgcgtgctc aaggatcaat agctggccat agctgcgaaa cggtccggcg aacaccacct
     5101 cgcctgcgcg cggcgccacc acttccgccc ccgcccgggc ggcgatccca agtccctggc
     5161 tgggctcgcc ggccccgcgc gcctcgccga accggcggac gatgtcgcca tgcaccggat
     5221 agggcaggct cgagccggtc ggcacctcgg gcaacgcggc gagccggcgc gcgccttcgg
     5281 cgggccgcgg cgccggcgga tcgggcttgg cgacggcggc ggtgatcgcc gggctcgcca
     5341 ccggctcgat ctcgttcaga cggtccgcct ctgcgacctc cgcggcggac cgctccgcgg
     5401 cctcggcgag cgcgtgatgc tcgaccgagc gccgcgccga gatctcggcc cgggccttgc
     5461 ggatgacctc gatcaggtcg cgcacgtcgc gcgcctgcgc gcccaacgcc ccgatgtcgc
     5521 gggcgaggtt gcgccggtcc gccatcagcg tcgcctgcag gctcgacttg cgctcgagca
     5581 gctggcgcag caccgcgcgc cggtcggcga cgtcggtgcg cagctcgacc agcgcggcct
     5641 ggcgcgaggt ctgctcggcg cgcagcgcgc gcaatgcggc gaggtcctcg gcgaggccga
     5701 tggtgcgctc gccgaactcg gtcgccgact gctccagcag gcgcacgccg tggaccgtgt
     5761 ccgaccactt gccgggccgc accagcagcg cctcgggcgg atggcgggcg atcagcacca
     5821 gcgcccccaa ggtcgccgcc aggccgccgc gcgtaagcgc gaggtcgcct ctcgcctcct
     5881 cgatccgcgc ctcgagcagc gccagatcgt cctcctggcg atgcagccgc agctcgaagt
     5941 cctggaccgc agcggcggcg tcgaccagct gctcgcgcaa ctcgtcgatt tcggcggcga
     6001 gatcgccggc acgccgctcg agtgcgctct gctcggcacg gcgcgcagcg agcgagcttt
     6061 cgatcacgtc gaggcgcgcc tgcgggtcgc ctgaggtcac gcccgcggcg ctcgcgcaca
     6121 cggcgtgcgc ggcaagcgcg gccgccgcca ggatcggggc tgcgcgcgcg ctactcggcg
     6181 gcacggcccg cggccgcctg gccggcgccg gcgatcagcg accggccggt catcgccgcc
     6241 ggccgggcga ggccgagcag ctcgagcacg gtcggcgcga tgtcggcgag ccggccgtgg
     6301 tcgaggcgcg ccccggccgg gccgccgacc agcaccaccg gcaccgggtt ggtggtatgg
     6361 gcggtgtgcg cctggcccga cgcccgatcg gacatctgct cggcgttgcc gtggtcggcg
     6421 gtgatcagca gcgcgccgcc ggcggcggtg accgcctcga ccagccggcc aaggcaggca
     6481 tcgaccgcct cgaccgcggc gatcgcggcg gcgagcttgc cggtgtggcc gaccatgtcg
     6541 gtgttggcgt agttgaccac gatcagctcg tggcgcccgg ccgccaccgc ctcgaccagc
     6601 cggtcggtca cctcgaacgc tgacatctcg ggcttgaggt cgtaggtcgc caccttgggc
     6661 gagggcacca ggatgcgctc ctcgccgtcg aaggtctcct cgcggccgcc gttgaggaag
     6721 taggtgacgt gggcgtactt ctcggtttcc gccagcctga gctggcgcag gcccgccctc
     6781 gccaccacct cgcccagcgt gtcggtgagc gcgagcggcg gaaagatgac gtcgaggaag
     6841 cggtcgaggc gggcagagta cgagaccatg ccgagcgcgg cggcgaaggc gacggtgcgc
     6901 gcgcgcgcaa agccgccgaa ttcggggtcg agcagcgcgg ccaggatctg gcgcacgcgg
     6961 tcggcgcgga agttggccat caccagcccg tcacggtcgg ccatgccgcc ataaccggcg
     7021 atcgccaccg gcgccacgaa ctcatcgccg atgcccgcgg cgtagctcgc ctcgaccgcg
     7081 gcgacggcgc tctgcgcatg ctcgccggcg gcgtcgacca gcacccgata ggcgcgcccg
     7141 gtgcgctccc agcgccggtc ccggtccatg gcgtagtagc gcccgccaac cgtcgccacc
     7201 gcaaccccgc cggcgccggc aaggtccgcg tcgaaccgcg ccagatagtc gcgcccgctc
     7261 cgcggcggcg tgtcgcggcc gtcgaggaag gcgtggaccc gcaccggcac gccggcggcg
     7321 gcaaccgtgc gcgccagggc ggcgatgtgg cgctggtggg aatggacgcc gcccggcgac
     7381 atcaggccga gcaggtggca ggcgccgccg gtggccttga ggcggtcgat caggcgggcg
     7441 agcgccggcg cgcgcgccag gctgccgtcg gcgagcgcgg cgtcgatgcg cggcagatcc
     7501 tgcatcacca cccggccggc gccgatgttc atgtgcccga cctcggagtt gcccatctgc
     7561 cccgccggca ggccgacctc gccggccgag gcgtcgatcc gcgcgtgcgg gcagccggcc
     7621 cacagccggt cccaggtcgg cgtgtcggcg agctcgatcg cgttgtcgtc ggcggcccgc
     7681 cggacgcccc agccgtcgag gatgcacagc accacgggac gcgtaccggc gaatgcggtc
     7741 atggcttgcc tgttatggcg cgctgcgccg gtgttcggtc cgcccggact agtggaccgc
     7801 gtgctcgggg agcggtaccg cccacatctt ctccagattg tagaaactgc gcacttcggg
     7861 ccggaacacg tggacgatca cgtcgccggc gtcgatcagc acccaatcgg cctgggccag
     7921 gccctcgacg ctcaacggcg ccgagccgcg ccgcttgagc cgctggacca ggtgctcggc
     7981 gatcgcggcg acgtggcgct gcgaccggcc gctggcgatg atcatgtagt ccgagatgtt
     8041 ggtcttccca gccaggtcga tgacggtgac atcctcggcc ttgtcttcct caagcgagtt
     8101 gcaggcgatg cgtgtcaacg catcgacccg cagcgattgg tcggcgtcgg ttatgtctcg
     8161 ctcctccctt ctgccatgcg cccatcgggc cgcccccagc cgccgctgtc gcgaatgcgg
     8221 gtagcggatg ccgggtgcag cccaccgccg atccaggccc atgccggcgg cggacagtcg
     8281 accagacggg ccggatcgcg caaccgcctg cgatagcggg caaatcggcg cgtcgccggg
     8341 cacagcagcg cgctgtgaga ataagtcggg cggtcgaata tcgcaatggg cagggcatgg
     8401 aacagggccg gccagcggtg ccagcgcggc atctggatca ggttgtcggc gcccatcagc
     8461 cagaccaggc gggcctgcgg cagccggcgc ttgagcgccg cgacggtatc gaccgtgtag
     8521 cgcgtgccga ggatgcgctc gatcgcggtg acccggatcg cgccgtggcc ggcgaccgcg
     8581 cgcgcgcgcg acaggcgcgc cgccagcggc gccatgcctg cggtcggctt gagcgggttc
     8641 tgcggcgaga ccagccacca gaccacgtcc agcccgagca gcgcctgcgc ccgccggctc
     8701 acgtggcggt ggccgtcatg ggccgggttg aacgagccgc ccagcaggcc gatgcgcggc
     8761 cgccgccggc cgggcggcag caacggcgca agctcgctgg cgagcgattt cagggccgcg
     8821 tctgcccgct gccgtggacg acgtacttgt agctggtcag ctgctcgagg ccgaccgggc
     8881 cgcgcgcgtg cagcttgccg gtcgagatgc cgatctcggc gcccatgccg aactcgccgc
     8941 cgtcggcgaa ctgggtcgag gcgttgtgca gcacgatgcc gctgtcgacc tcgttgagga
     9001 agcgctcggc cgcggccaca tccgcggtga cgatgctgtc ggtgtggtgc gagccgtgac
     9061 gctcgacatg ggcgatcgcc tcgtcgaggt cggcgaccgt cttgatcgag atgatcgcgt
     9121 ccaggtactc ggtgtcccag tccgcaggcg tcgccggctt gactcgcgca tcgatgcgct
     9181 gcaccgcgtc gtcgccgcgc acctcgcagc cgccgtcgag gaaccggcgc accaggcgcg
     9241 gcagcaccgc gccggcggca tcgcggtgga ccagcacggt ctcggccgcg ccgcagatgc
     9301 cggtgcgccg catcttggcg ttgaagctca cctcttccgc catcgccggg tcggcggcgc
     9361 ggtcgatgta gacgtggcag atgccgtcca ggtgcttgat caccggcacc cggctgtcgg
     9421 cggcgacgcg cgcgaccagc gacttgccgc cgcgcggcac caccacgtcg atgtagtcgg
     9481 tcatggtcag catctcgccg accgcggcgc ggtcgcgggt gggcaccagc tggatcgcgg
     9541 cctcgggcag acccgccacc ttgagcccgt ggacgaggca gcggtggata gcgcgcgagg
     9601 agtggaagct ctccgagccg ccgcgcagga tcacggcatt gcccgacttc aggcacagcc
     9661 cgccggcatc cgcggtgacg ttggggcgtg attcgtagat gatcccgatc acccccagcg
     9721 gcacccgcac gcgcgtgatc ctgaggccgt tgggccgggt ccactcgccg atcacctggc
     9781 cgaccgggtc gggcagcccg gcgacgtcct cgagcccctc cgccatcgcc tcgatgcgcg
     9841 ccgcgtccag cgccagccgg tcgaggaagg cctcggtctg gttgcttttg cgcgccgccg
     9901 ccaggtcatc ggcattggcc gcgagcagct cgttcgcgcc ggcgcggacc gcgccggcgg
     9961 cagcattgag ggcggcgttc ttggccgcgg tctcgacgcg cgcgagctgc gccgcggcgg
    10021 cgcgcgcggc gcggccgatg gccagcatct gggcgtggat gtcgccctcc gtcgtctgcc
    10081 gtgcgaggct catttttgct cccggtccgc ctcgcggacc agcaccaggt cgtccctgtg
    10141 gatcatctcc tcgcggccac gatagccaag gatctgctct atttcacggc ttttgtggcc
    10201 gaggatgcgg cgggcgtcgg cgcaggcata ggcgctgagg ccgcggccga gctcggttcc
    10261 cgcccggtcg cgcaccagca ccaggtcgcc gcgccggaag ctgccctcgg cggccagcac
    10321 gcccgccggc agcaggctgt tgccctggcc gagcgcgcgc gcggcgccgt cgtcgaccac
    10381 cagcgcgccg gccggctgca gggtgccggc gatccagtgc ttgcgcgcgg tgagcgggct
    10441 ggcatgggcg aggaaccagg tgcacggcgc gccggcgagc aggcgctcga tcgggtgcgc
    10501 cgccatgcct tccgcgatca ccatgtggca gccggcgcgg accgcgttgc gcgccgcggc
    10561 gagcttggtg atcatgccgc ccgagccgct gtccgacagg ctggcgccgg ccatgcgctc
    10621 gacctcgggc gtgatctcgt cgatctcatc gatccggcgc gcgctcgcat cgaggcgcgg
    10681 gtcggcagtg tacagcccgt cgatgtccga cagcagtatc agtacctcgg cgctcgccat
    10741 ctgggcgacg cgcgcggcca ggcggtcgtt gtcgccgaac cggatctcgg cggtggcgac
    10801 ggtgtcgttc tcgttgatca ccggcagtgc gccgagcttg agcagggatt cgagcgtggc
    10861 gcgggcgttg aggtagcgcc ggcggtcctc ggagtcctcg agggtcagca acagctgggc
    10921 gacggtgatg ccgtggcggt cgagcgcctg ctgccaggcg tgggcgaggc ggatctggcc
    10981 gaccgcggcg caggcctgct tgtcctcgag cctgagctcg cgccggccga ggccgaggcg
    11041 gcggcggccg accgcgatcg cgcccgagga caccagcgcg acctgctggc cgcgcgcgcg
    11101 catggcggcg acgtcgtcgg cgagcgccgc cagccaggag tcgcgcaggc gcccgtcgtc
    11161 gccctcggtg agcagcgacg agccgatctt gatcaccacc cggcgcgcct tcgccagccg
    11221 ctcggtcatg gctgccgggg ctcctccgct gccggcgcgc gcgccgcggc gaccaggtcc
    11281 atcagcgcgc cgatcacctc gggcacgccc tcgccggtcg ccgccgacag cgccagcacc
    11341 cgctcggccc cggcggcggc gagcgcgcgc tcggcggcgc gctcggccgg cgcatccagc
    11401 aggtcgcact tgttgagccc gaccagcgcc ggcttgccgg cgagcccgtg gccataggcc
    11461 ccgagctcgc cgcggatggt gcggtaggcc gcggccacgt cctcgccggc gccgtcgacc
    11521 aggtgcagga tcgcagcgca gcgctcgatg tggccgagga agcggtcgcc gaggccgcta
    11581 ccggcatgcg cgccctcgat caggccgggg atgtcggcga tcacgatctc gcgctcgccc
    11641 acgcgcgcca cgccgagctg cggggtcagc gtggtgaacg ggtagtcggc gatcttgggc
    11701 cgggcgcggc tcgccgcggc gaggaaggtc gacttgccgg cattgggcag cccgaccagg
    11761 ccgacatcgg cgatcagctt gaggcggagc cacagccagc gctcctcgcc cgcacgcccc
    11821 ggctcggcct cgcgcggcgc ctggttggtc gaggtcttga aggcggcgtt gccgcgcccg
    11881 ccgcgcccgc cctcggcgag cacccgccgc tcgccgggct cggtcatgtc ggcgatcacg
    11941 gtcgccttgt cctcggccag gatctcggtg ccgaccggca cccggatcac cagcacgtcc
    12001 gcgctcgccc cggtgcgctc gctgcccgag ccattggcgc cgggccgggc gcggaagtgc
    12061 tggcggtagc ggaagtcgat cagggtgttg aggtcgtccg cggcgaccgc caccacgtcg
    12121 ccgccgcggc cgccgttacc gcccgatggc ccgccgtagg cgacgcgctt ctcgcgccgg
    12181 aaggcgacgc agccgttgcc gccgtcgccc gccttcacgt agatcttggc ctggtcgaga
    12241 aaccgcatcg cccggacatc cggagtaggc gccgctgctg cggaggacgg gtcggctgcg
    12301 agggccggat acgaaaaagg gaatggcccg gcgccattcc ccgcccgcaa cggcgcccgg
    12361 cgtggccgct cagccggccg gctccaccga cacgaaggtc cgcccgagcc gcccgtggcg
    12421 gaactgcacc cggcccggcg acagcgcgaa cagggtgtgg tcggtgccga tgccgacatg
    12481 gtcgcccggg tggaacttgg tgccgcgctg gcggaccagg atgttgccgg gcaccacgat
    12541 ctggccgccg aaccttttca cccccagcct gcggccggcg gaatcgcggc cgttgcgcga
    12601 gctgcctccg gctttcttgt gggccatcgc ggtctactcc tcggtctttg cggccggcgc
    12661 ggccggcgcc gtggcgagcg tgatgtcggc gatccggacc acggtctgcg cctggcggtg
    12721 gccgcgggtg cggcggtagt ttttgcgccg cttcttcttg aacacgatga tcttctcgcc
    12781 cagcgcctgc tcgagcacgg tggcggtgac gcgggcgccg gcgacggtcg gcgcgccggc
    12841 ctgcaccccg ccctcgcccg cgaccaggag cacgtcctcg aacgcgacct cgccgcccgc
    12901 cgcgccggcg agcttctcga ccgcgatgac gtcgttcctg gccacccggt actgcttgcc
    12961 gccggtgcgg atgactgcgt acatggcgcg ctccttggcc gcgggactgc tcagtgccgc
    13021 gggagtcgag gagccggcac tatagccgcc gcgcctcgcc tgtcaacggc gcgggccggc
    13081 gcggcgcggc gtccgtggcc ggcttgacgg ccggccgtcg cttgcccgcc gccgggcctt
    13141 gggctagtgt ccgccgccgt cgcgcccggg ccgccgccgg gcccgcgcgg aggggtgccg
    13201 gagtggtcga tcggggcggt ctcgaaaacc gttgtacctt cgggtaccgt gggttcgaat
    13261 cccaccccct ccgccagccg gccgcgttac gtttgacgtc cgcgccgtgc gctttcgcga
    13321 tgtccgcgtg aagcgggggg cggtggctca tctgcggcgc gcgcgccggc cttgactc
//
LOCUS       DASWHU010000104         8617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1897476, whole genome shotgun sequence.
ACCESSION   DASWHU010000104 DASWHU010000000
VERSION     DASWHU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897476"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..3095
                     /locus_tag="VGB88_03700"
     CDS             21..3095
                     /locus_tag="VGB88_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646575.1"
                     /translation="MKMVLALLALVLALPALAAQPEELVPDETGEVRVPLAAYTAMLN
                     QLTAQPRPAPAAYAIGQAEISVAVSERDGRVTATVDATFQIETFEPEWTLVQVLPPGA
                     ALRAASVNGAPVRLVEGPDGLGWSTDRAGTVTMRLAYGVDATRHDSGYALPLPMPRAA
                     AANFTLTYPGTGIDLAVIPSADMTVEELDGATRLTAAVPATSSVLVTWRAPDQRPFAI
                     SRAHYQGELSDEALHWTAELEVEAFTGERITLPVMPNSVTLSEVRVDGQPTTVLDEDG
                     SFAVRLQGRGMHRIAVAFQAPLAGADGAPQARLAIPRIPVSRFDLTLPGRKQVTVVPA
                     AAVMTTELDEATKATAYIPMGDSVLFTWTEAIPEELRVELRANASLYHRLHADEGVLH
                     GLATIVYEITHGEAGVLELDIPGDAQVNRIDAPAGGVSDWAVVESDVPGLKAITVFLD
                     RPVTGEYVLEVSYERLLGARPDAEAPIAVPLLSARNVQRQRGMVALLSGPELTLEPVT
                     EASLSKVGENQLPAFIRDQITMTVAHTYKYTEARPELTVAAVAPERKQGVFDAQVDTL
                     ISLGDVTMRGSATVEIDVKSGTIQELALRVPAQVNVLGVSGPSLRTHQVRAADGAQAI
                     DLAFTREMEGQFRIEVNYERIMDGDAPESGVATVSVDGAEVEHGRIAIEALTAVEVQA
                     ATVDQLTGLDINELPQQLVLKTTNPILLAYRYVRAGVPVRLGLKITRHREIDVQVAVI
                     ETAEYGSLFTGDGLAVTSARLTVRNSRKQFLRLALPAGSEVWSVFVDGKPEKPAYAGN
                     GAAGANESVLIKMINSAEGFPVDIVYATPVASIDHFGALASRLPRPDMVVTRTRWDVF
                     LPVGPSYFTPDSTMDITVEGVPANPRATPVNAVALASDALRAQMGQPLRIVVPTQGIR
                     FAFEKLYADQSSDEAEFTIRYVSEDANSVGLALSAAGAVLLWIGVVAIGGRRLRLNRA
                     ATIACVASGAALVALSIGYLGADPLLASGVALLIAVALAAWWGVERWRTRAA"
     gene            3201..4220
                     /gene="dctP"
                     /locus_tag="VGB88_03705"
     CDS             3201..4220
                     /gene="dctP"
                     /locus_tag="VGB88_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9646576.1"
                     /translation="MTRLLGTAVAGIALLLVGAAAGAQDYELTLAMLPAPGTTYFAMT
                     STVPERIAKATDGRVKVTLNDSLVGGPQIAAAVRDGRVPMSAAIHTYLAAEEPRMGLF
                     NLPGLIDDMAEYKFVCDAFWCDDLAKLWREKWNAVVLASGAWCTQQLFSKEPIHSVED
                     FKNKRLRVHNPQTAELMDALGAKPTPLPLSEVMPALERGVIDGLFTSTCYGWRQEYWR
                     LAKNVQNWMLGPITGWAIIVNADSWAEIPADLQDKIRATMKELEHDAFYGYYDFVRTA
                     MADMESKGVAFWVAPRAEHDRIFDPQYTAPVFAAWVARAKEVGFDGEAYLKRVREVLG
                     KDLDR"
     gene            complement(4227..5009)
                     /locus_tag="VGB88_03710"
     CDS             complement(4227..5009)
                     /locus_tag="VGB88_03710"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX9646577.1"
                     /translation="MLNGRCALVTGSTAGLGRAIAESLAGAGCDIVLNGFGERDDIEA
                     QRRALEADHGVKALHHGADLSKPGEIEDLMACAADAFGAVDVLVNNAVVRHFAPVDEF
                     PVARWDEAIAVNLSAAFHTIRLAVPAMRARNWGRIVNIASVYGLIAVRHRVDYVTTKT
                     ALIGLTRAVALDVVGHDITCNAICPGAMLTPAIEARIAETAAADGVTQDEAIARFLAS
                     RQPTGRFIAPENVAALVCFLCGPAGRDITAAALPVDSGWTAT"
     gene            complement(5060..6442)
                     /locus_tag="VGB88_03715"
     CDS             complement(5060..6442)
                     /locus_tag="VGB88_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9646578.1"
                     /translation="MASPAGALAAWAGELAFADLPADVVETTKLRLLDVIGLALAGLG
                     TDFGESVRAGACALDAGGPARILGSGDAVGTATAALANGALAQALEFDDTHNESIVHI
                     SAPSVAAALALAEARPVSGRELIAAVAVGNEISCRVGSVAPGQFHRRGFHPTGLFAAF
                     GATYLAGRVLGLSAAQMANAAGIAGSFAAGILQCWVDGTQSKYLHPGWAAHGGLVAAH
                     LAQAGTTGPEAVFEGRFGLFASHLQDEAVACDFARITGGLGTLWESRKASFKPYPAAH
                     VIHPYIDALLRLRRAHAIDAAKVREIVVPVASYIVGIVCEPAAEKRRPRSDSHARVSL
                     PYTLAEALARGRLARDAYARDALADPEILRLADAVRIEVDPDFPGPERFKGQVRIVMA
                     DGTIFEEVEEHNHGSAENPMTEGEIIAKFDDNVADVLTPDRRRRLVETALAAELLDSA
                     RVLTDLSIRS"
     gene            complement(6460..7704)
                     /locus_tag="VGB88_03720"
     CDS             complement(6460..7704)
                     /locus_tag="VGB88_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646579.1"
                     /translation="MASRWHDALRGAGILAAAAALALAAGTTGAAAAGTVQVGLLVPL
                     SGPWARQGQVMREGAELAVEHINAAGGIAALDGARLELVVFDAGDSVERAKNAAQRMV
                     AEAPDLVAACGSFLSSFTLAVTEVTERAALPVITLSYSDQITERGYAFVFQSSATGAT
                     QAAQALPVLVKLAEDASGTRPKTVAIIKDNTAASLAFVKPMQAGGLESLGLELVVDET
                     YTPPLADATPLIQRLRAARPDLLLLLPTVISDAKLLLEKMAEFGLGRGHLPTISNGTA
                     IADPDLLNTMNVDLLEGVMSIVANWGAKGQEQLIEAFKAKSGEPWMIQNPLSTYGHIW
                     IVKQALESAGIADRRAVARAIREMDLTDGPAAYFPGHRVAWDEKGRRIGAGLLVVQWQ
                     GGEPVTVYPPESAVATPAWPQR"
     gene            complement(7762..8469)
                     /locus_tag="VGB88_03725"
     CDS             complement(7762..8469)
                     /locus_tag="VGB88_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646580.1"
                     /translation="MLEIERLSAGYGGRAVLDEVSLEVGRGEFVAIVGPNGAGKTTLF
                     KTISGILRPSSGAIRFQGADLLAVAPARRAHLAIAHVPEGRQVFPSLTVIENLEMGAY
                     TARRRAEWPSTLAQIFDWFPILAERRRQLAGTLSGGQQQMLAIGRGLASAPALLMLDE
                     PSMGLAPVVADEIFERIVDIHRGAGVTVLLVEQRVAEALHFADRGYVLEAGRIVLSGA
                     RAELESDDRIRRAYLGM"
     gene            complement(8462..>8617)
                     /locus_tag="VGB88_03730"
     CDS             complement(8462..>8617)
                     /locus_tag="VGB88_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646581.1"
                     /translation="IEHTMGAMVRLVDRFVVLDHGAVLAEGAPAAITSDARVIEAYLG
                     KKWITHA"
BASE COUNT         1261 a         3085 c         3057 g         1214 t
ORIGIN      
        1 ccggcccgga ggatccgacc atgaaaatgg tgcttgcctt gctcgccctg gtgctcgcgt
       61 tgccggcact cgcggcgcag cctgaggagc tggtgcccga tgaaacgggc gaggtgcgcg
      121 tgccgctggc ggcgtacacg gcgatgctca accagctcac cgcccagccg cgccccgcgc
      181 ccgccgccta cgccatcggc caggccgaga tttcggtcgc ggtgagcgag cgcgacggcc
      241 gggtcacggc gacggtcgat gcgaccttcc agatcgagac cttcgagccc gagtggacgc
      301 tggtccaggt gctgccgccc ggcgcggcgt tgcgcgccgc gagcgtcaac ggcgcgccgg
      361 tgcgcctggt cgaggggccg gacggcctcg gctggagcac cgaccgcgcc ggcacggtga
      421 ccatgcggct cgcctacggc gtcgatgcga cccgccacga cagcggctac gcgctgccct
      481 tgccgatgcc gcgcgccgcg gccgccaact tcaccctgac ctatccgggg accggcatcg
      541 atctggcggt gatcccgtcg gccgacatga cggtggagga acttgacggc gcgacccgcc
      601 tcaccgcggc ggtgccggcg acatcgtcgg tgctggtcac ctggcgggcg cccgaccagc
      661 gcccgttcgc gatcagccgc gcccactacc agggcgagct cagcgacgag gcgctgcatt
      721 ggaccgcgga gctcgaggtc gaggccttca ccggcgagcg catcaccttg ccggtgatgc
      781 cgaacagcgt caccttgagc gaggtccgcg tcgacggcca gcccacgacc gtgctcgatg
      841 aggacggcag cttcgcggtg cgcctgcaag ggcgcggcat gcaccggatc gccgtcgcct
      901 tccaggcgcc gctcgccggc gcggacggtg ccccgcaggc acgcctggcg attccgcgca
      961 tccccgtctc caggttcgac ctgaccctgc cggggcgcaa gcaggtgacg gtcgtgcccg
     1021 ccgccgcggt gatgacgacc gagctcgacg aggcgaccaa ggcgaccgcc tacatcccga
     1081 tgggcgacag cgtgctgttc acctggaccg aggcgatccc cgaggagctg cgcgtcgagc
     1141 tgagggcgaa cgcgagcctc tatcatcggc tccacgccga cgagggcgtg ctgcacggcc
     1201 tggcgacgat cgtctacgag atcacccacg gcgaggccgg cgtgctcgag ctcgacatcc
     1261 cgggcgacgc gcaggtcaac cgcatcgacg cgccggcggg gggcgtgtcg gactgggcgg
     1321 tggtcgagtc ggacgtcccg gggctgaagg cgatcaccgt cttcctcgac cgcccggtca
     1381 ccggcgagta cgtcctcgag gtgtcgtatg agcgcctgct cggcgcgcgg cccgatgccg
     1441 aggcgccgat cgcggtgccg ctgctgagtg cgcgcaacgt ccagcgccag cgcggcatgg
     1501 tggcgctgct gtcgggaccc gagctcacgc tcgagccggt gaccgaggcg agcctgtcca
     1561 aggtcggcga gaaccagctg cccgccttca tccgcgacca gatcaccatg accgtcgccc
     1621 acacctacaa gtacaccgag gcgcggcccg agctcacggt tgcggcggtc gcgccggagc
     1681 gcaagcaggg cgtgttcgac gcccaggtcg acaccctgat ctcgctcggc gacgtgacca
     1741 tgagaggctc ggcgacggtc gagatcgacg tcaagtccgg caccatccag gagctcgccc
     1801 tcagggtccc ggcgcaggtc aacgtgctcg gcgtatccgg cccgtcgctc cgcacccatc
     1861 aggtgcgcgc ggccgacggc gcgcaggcaa tcgatctcgc gttcacgcgc gagatggagg
     1921 ggcagttccg catcgaggtc aactacgagc ggatcatgga cggcgacgcg cccgagtcgg
     1981 gcgtcgccac ggtctcggtc gacggcgccg aggtcgagca cggccgtatc gcgatcgagg
     2041 cgctgaccgc ggtcgaggtc caggcggcca ccgtcgacca gctcaccggc ctcgacatca
     2101 acgagctgcc gcagcagctg gtgctcaaga ccaccaaccc gatcctactc gcctaccgct
     2161 acgtgcgcgc gggcgtaccg gtgcggctcg ggctcaagat cacccgccac cgcgagatcg
     2221 acgtccaggt cgcggtcatc gagaccgccg agtacggctc gctgttcacc ggcgacggcc
     2281 tggcggtgac cagcgcgcgg ctgaccgtgc gcaacagccg caagcagttc ctgcgcctcg
     2341 ccctgccggc gggctcggag gtgtggtcgg tgttcgtcga cggcaagccg gagaagccgg
     2401 cctatgccgg caacggcgcc gccggcgcca acgaatccgt gctaatcaag atgatcaatt
     2461 ccgcggaagg cttcccggtc gatatcgtct acgccacgcc ggtggcgagc atcgaccact
     2521 tcggtgcgct cgcaagccgc ctgccgcgcc ccgacatggt ggtcacccgc acccgctggg
     2581 acgtgttcct gccggtggga ccgagctact tcacgcccga cagcaccatg gacatcaccg
     2641 tggagggcgt ccccgccaat ccgcgcgcga ccccggtcaa cgcggtggcg ctcgcctcgg
     2701 acgcgctgcg cgcccagatg ggccagccct tgcgcatcgt cgtgccgacg cagggcatcc
     2761 ggttcgcgtt cgagaagctc tacgccgacc agtcctccga cgaagccgag ttcacgatcc
     2821 gctacgtctc ggaggacgcc aatagcgtcg gcctggcgtt gagcgccgcc ggtgcggtgc
     2881 tgctgtggat cggggtggtc gcgatcggcg gccgccggct caggctgaac cgggccgcga
     2941 ccatcgcctg cgtggcctcg ggcgcggcgc tggtggcact gtcgatcggc tatctgggcg
     3001 cggacccgct gctcgcatcc ggcgtcgcgc tgctgatcgc ggtcgcgctc gcggcgtggt
     3061 ggggagtcga gcgctggcgc acgcgcgccg catagccgat gcgcggagct tccggggccg
     3121 ccccctgtca ttgccgccgc cgcctgtcgt atgatcgacg gcgaagggtc gcaagaaggc
     3181 ccgcagggag gaatcgttta atgaccagac tgctcggcac cgccgtcgcc ggcatcgccc
     3241 tgctgctcgt gggcgcggcg gcgggcgcgc aggactacga gctcacgctg gcgatgctgc
     3301 cggcgcccgg caccacctat ttcgccatga cgtcgaccgt tcccgagcgg atcgccaagg
     3361 cgaccgacgg gcgggtcaag gtgaccttga acgattcgct ggtcggcggc ccgcagatcg
     3421 ccgccgcggt gcgcgacggc cgcgtgccga tgtcggccgc cattcacacc tacctcgccg
     3481 ccgaggagcc gcgcatgggc ctgttcaacc tgccgggcct gatcgacgac atggcggaat
     3541 acaagttcgt gtgcgacgcc ttctggtgcg acgacctcgc caagttgtgg cgcgagaagt
     3601 ggaacgcggt ggtgctcgcc tcgggcgcgt ggtgcaccca gcagctgttc tcgaaggagc
     3661 cgatccactc ggtcgaggac ttcaagaaca agcggctgcg cgtccacaac ccgcagaccg
     3721 ccgagctgat ggacgcgctc ggcgccaagc cgacgccgct gccgttgtcc gaggtgatgc
     3781 cggcgctcga gcgcggcgtc atcgacggcc tgttcacctc cacctgctac ggctggcggc
     3841 aggagtactg gcggctggcc aagaacgtcc agaactggat gctcggcccg atcaccggct
     3901 gggcgatcat cgtcaacgcc gacagctggg ccgagattcc cgccgacctg caagacaaga
     3961 ttcgcgcgac catgaaggag ctcgagcacg acgccttcta cggttactac gacttcgtgc
     4021 gcacggcgat ggccgacatg gaatccaagg gcgtcgcctt ctgggtcgcg ccgcgcgccg
     4081 agcacgaccg catcttcgat ccgcaatata ccgcgccggt gttcgcggcg tgggtggcgc
     4141 gggcgaagga ggtcggcttc gacggcgagg cgtacctgaa gcgcgtgcgc gaggtgctgg
     4201 gcaaggacct cgaccgttag gctcggtcag gtcgcggtcc agcctgaatc gaccggcagc
     4261 gccgcggcgg tgatgtcgcg ccccgccggg ccgcacagga agcagaccag cgcggcgacg
     4321 ttctccggtg cgatgaagcg ccccgtaggc tggcgcgagg caaggaagcg ggcgatcgcc
     4381 tcgtcctgcg tcacgccgtc cgccgcggcg gtctcggcga tgcgtgcctc gatcgcgggc
     4441 gtcagcatcg cgcccgggca gatcgcgttg caggtgatgt cgtggccgac cacgtccaac
     4501 gccaccgcgc gggtcaatcc gatcagcgcg gtcttggtgg tcacgtagtc gacccggtgc
     4561 ctgaccgcga tcaggccgta gaccgaggcg atgttgacga tccgccccca gttgcgcgcg
     4621 cgcatcgccg gcacggcgag gcggatggtg tggaacgcgg ccgacaggtt gactgcgatc
     4681 gcctcgtccc agcgcgcgac cgggaactcg tcgacgggcg cgaaatggcg cacgaccgcg
     4741 ttgttgacca ggacgtcgac cgcgccgaag gcatcggcgg cgcacgccat caggtcctcg
     4801 atctcgccgg gcttggagag atcggcgccg tggtggagcg cctttacgcc gtgatcggcc
     4861 tcgagcgcgc gacgctgcgc ctcgatgtcg tcgcgctcgc cgaagccgtt cagcacgatg
     4921 tcgcagcccg cgcccgccag gctttccgcg atcgcgcggc cgaggcccgc ggtcgatccc
     4981 gtgaccagcg cgcaccgccc gttcagcatc gggatgctcc tctaaccatg atcatgttcg
     5041 ctggctaacg attagtgcat caggagcgga tcgacaggtc ggtgaggacg cgcgcgctgt
     5101 cgagcaactc ggcggcgagc gccgtctcga ccagccgtcg gcgccggtcg ggcgtgagca
     5161 catcggccac gttgtcgtcg aacttggcga tgatctcgcc ctccgtcatc gggttctcgg
     5221 ccgagccgtg gttgtgctcc tcgacctctt cgaagatggt gccgtcggcc atgacgatgc
     5281 gcacttggcc cttgaaccgc tcggggcccg ggaagtcggg gtcgacctcg attctcacgg
     5341 cgtcggcaag gcgcaggatt tccgggtccg cgagcgcgtc ccgcgcatag gcgtccctgg
     5401 cgaggcggcc gcgcgccagc gcctcggcga gcgtgtaggg caggctcacc cgggcgtggg
     5461 aatcgctccg cggccggcgc ttctccgccg ccggctcgca cacgatcccg acgatgtagc
     5521 tcgccaccgg caccacgatc tcgcgcacct tggcggcatc gatcgcgtgc gcacgccgca
     5581 acctcagcag cgcgtcgata taagggtgga tgacgtgcgc cgcggggtag ggcttgaacg
     5641 acgccttgcg gctctcccac agcgtgccga ggccgccggt gatgcgggcg aagtcgcacg
     5701 cgacagcctc gtcctgaagg tgcgaggcga acagcccgaa gcggccctcg aacaccgcct
     5761 cggggccggt ggtgccggcc tgggcgagat gggccgcgac cagcccgcca tgcgccgccc
     5821 agccgggatg gagatacttc gactgggtgc cgtcgaccca gcactgaagg atgccggcgg
     5881 cgaagctgcc ggcgatgccg gcggcgttgg ccatctgtgc cgcgctcagg ccgagcacgc
     5941 gcccggccag ataggtcgcg ccgaaggcgg cgaacagccc ggtcgggtgg aagccgcggc
     6001 ggtggaactg gcccggcgcg acgctgccca cccggcacga gatctcgttg ccgaccgcga
     6061 ccgcggcgat cagctcgcgc ccgctcaccg gccgcgcctc ggcgagggcg agggcggcgg
     6121 cgaccgaggg ggcgctgata tggacgatcg actcgttgtg ggtgtcgtcg aactcgagcg
     6181 cctgtgcgag cgcgccgttg gcgagcgcgg cggtggccgt gccgaccgca tcgcccgacc
     6241 cgagaatgcg cgccggcccg cccgcatcga gcgcgcacgc cccggcgcgc accgactcgc
     6301 cgaagtccgt gccgagcccg gcgagcgcga ggccgatcac gtcgagcagc ctgagcttgg
     6361 tggtctcgac cacgtccgcc ggcaggtcgg cgaaggccaa ctcgccggcc cacgccgcca
     6421 gcgcgccggc ggggctcgcc atggcgcggg cctccggccc tagcgctgcg gccaggccgg
     6481 cgtggcgacc gccgactcgg gcgggtagac cgtgaccggc tcgccgccct gccattgcac
     6541 caccaagagc ccggcgccga tccgccgccc cttctcgtcc cacgccaccc ggtggcccgg
     6601 gaagtaggcg gccgggccgt cggtcaggtc catctcgcgg atcgccctgg ccaccgcgcg
     6661 ccggtcggcg atgcccgcgc tctcgagcgc ctgcttgacg atccagatgt ggccgtaggt
     6721 cgacagcggg ttctggatca tccacggctc gcccgacttc gccttgaacg cctcgatcag
     6781 ctgctcctgg cccttggcgc cccagttggc gacgatcgac atcacgccct cgagcaggtc
     6841 gacgttcatg gtgttgagca ggtcgggatc ggcgatcgcg gtgccgttgg agatggtcgg
     6901 caggtggccg cggccgaggc cgaactcggc catcttctcg agcagcagct tggcgtccga
     6961 gatcacggtc ggcagcagca gcagcagatc cgggcgcgcc gcgcgcaggc gctggatcag
     7021 gggcgtcgcg tcggcgagcg gcggggtgta ggtctcgtcg accaccagct cgaggccgag
     7081 agactcgagc ccgcccgcct gcatcggctt gacgaaggcc agcgaggcgg cggtgttgtc
     7141 cttgatgatc gcgaccgtct tgggccgcgt gccggacgcg tcctcggcga gcttgaccaa
     7201 caccggcagc gcctgcgcgg cctgggtggc gccggtcgcc gaggactgga acacgaaagc
     7261 atagccgcgc tcggtgatct ggtccgagta ggacaaggtg atcaccggca gggcggcgcg
     7321 ctcggtgacc tcggtcaccg cgagcgtgaa cgagctcagg aacgagccgc acgccgcgac
     7381 caggtccggg gcctcggcga ccatgcgctg ggcggcgttc ttggcccgct cgacgctgtc
     7441 gccggcatcg aacaccacca gctcgagcct ggcgccgtcg agcgcggcga tgccgcctgc
     7501 cgcattgatg tgctcgaccg cgagctcggc gccctcgcgc atcacctggc cctggcgcgc
     7561 ccacggtccc gacagcggca ccaagaggcc gacctgcacc gtccccgccg ccgccgcccc
     7621 ggtcgtgccc gcggcgagcg cgagtgccgc cgccgccgcc aggatgcctg cgccgcgcaa
     7681 cgcatcgtgc caccttgacg ccatgatctc ctcccctcga ttcggatttc cggcagactc
     7741 gtaacagatg ccgccgtgcc gtcacatacc caggtaggcg cggcggatgc ggtcgtcgct
     7801 ctcgagctcg gcgcgcgcgc ccgacagcac gatccgcccg gcctcgagca catagccgcg
     7861 gtcggcgaaa tggagcgcct cggcgacccg ctgctcgacc agcagcacgg tgacgccggc
     7921 gccgcggtgg atgtcgacga tgcgttcgaa gatctcgtcg gcgaccaccg gcgcgagacc
     7981 catcgacggc tcgtcgagca tcagcagcgc cggcgccgag gcaagcccgc gcccgatcgc
     8041 gagcatctgc tgctggccgc ccgacagcgt gccggcaagc tggcggcggc gctcggcaag
     8101 gatcgggaac cagtcgaaga tctgcgccag cgtgctcggc cactcggcgc gccggcgcgc
     8161 ggtgtaggcg cccatctcga ggttctcgat caccgtgagc gaggggaaca cctggcgccc
     8221 ctcgggcacg tgggcgatgg cgaggtgggc gcggcgcgcc ggcgccaccg ccagcaggtc
     8281 cgcgccctgg aagcggatcg cgccggaact cgggcgcagg atgcccgaga tggtcttgaa
     8341 cagggtggtc ttgccggcgc cgttggggcc gacgatggcg acgaactcgc cgcgcccgac
     8401 ctcgagcgac acctcgtcca gcaccgcgcg gccgccatag cccgccgaca ggcgctcgat
     8461 ctcaagcatg cgtgatccac ttcttgccga gataggcctc gatcacgcgc gcgtcgctgg
     8521 tgattgcggc gggcgcgccc tcggcgagca ccgcgccgtg gtcgagcacc acgaagcggt
     8581 cgaccaggcg caccatggcg cccatggtgt gctcgat
//
LOCUS       DASWHU010000105         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3006096, whole genome shotgun sequence.
ACCESSION   DASWHU010000105 DASWHU010000000
VERSION     DASWHU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006096"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..556
                     /locus_tag="VGB88_03735"
     CDS             116..556
                     /locus_tag="VGB88_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX9646582.1"
                     /translation="MSGREAGSDAVQWEEARRWFAQADDDIRVAWACLAAAPPIVGAA
                     ALHCQQAAEKTLKGLLIAARQKTGKTHNLGELTTAVAAQYAPFRDDLDWLRELTEWYL
                     GMRYPDIEIERPPAAAAVSTALESVSRLLERARSLDPTRTATPK"
     gene            complement(584..832)
                     /locus_tag="VGB88_03740"
     CDS             complement(584..832)
                     /locus_tag="VGB88_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9646583.1"
                     /translation="MAKVVLPASVGQQFAGGETEIEVAGATVRAIVRALEARHPGLGA
                     AIEQGMAIAIDGEIFQDPYLEPVAADSEVFILPKIGGG"
     gene            complement(837..3107)
                     /locus_tag="VGB88_03745"
     CDS             complement(837..3107)
                     /locus_tag="VGB88_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008280775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9646584.1"
                     /translation="MNDVSNGYKWVGKRTIRPDGPDKVTGRAKFGADLRLPGMLWGKV
                     LRSPHAHARIISIDTAKAKALPGVKAVITGADLPPPPAPKWEAAGEAQVNFYDMARNV
                     MARDKVLYQGHALAAVAATTSAIAREALSLIEVKYEVLAHVIDPVAAMAKDAPLLHDD
                     LYTDGVSPKPDQPSNVAKRVEFALGDVAAGFKKAEVVVELDFTTKPVHQGYIEPHAVV
                     ADASEDGQVQVWCSSQGQFMVRSYVARLLGMDVADIRVTPAEIGGGFGGKTVVYLEPL
                     AVLLARRSGRPVKMMMSREEVFLASGPTSGAAGKIKIGATRDGRITAAEATLMYQAGA
                     FPGSPIGPGCMTAFAPYDLENVRVVGYDVVVNRPKVAAYRAPGAPISAFGVESALDEL
                     AKKLGMDPLDLRQKNGAHEGTKAAYGPKFGPIGFIETLEAARSHPHYAAPLGPNQGRG
                     VASGFWFNIGGESAATVNVNEDGTVTVVEGNPDIGGSRAAMANMVAEVLGVDVTKVRP
                     VVADTASVGFNFLTGGSRVTFASGKVAVDATRKVVAELKARAAKTWEIDPDAVEWADG
                     AAHPAGPNAGKFKPLSLAEIAAKSARTGGPIVGQATENVKGAGPGFGTHICDVEVDRE
                     TGRVTILRYTAIQDVGRAIHPGYVEGQLQGGAAQGIGWALNEEYIYGEDGRMQNPGFL
                     DYRVPVASDLPMIDTVLVEVPNPNHPFGVRGVGEVPIIPPMAAVANAIEAATGVRLHD
                     LPMSPPKVLAALRNGH"
     gene            complement(3111..3587)
                     /locus_tag="VGB88_03750"
     CDS             complement(3111..3587)
                     /locus_tag="VGB88_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9646585.1"
                     /translation="MARHHVTTTVNGDDCEFLCDTEQTLLDALRDELGLTGSKEGCAS
                     GDCGACSVIFDGRLVCACLVLGVEAEGRTVETIEGMARGEHLHPLQQKFLEEAALQCG
                     FCTPGFLIAAKALLDRNPDPTETEVRYWLAGNLCRCTGYDKIVRAVMDTAAEMRGA"
     gene            complement(3587..>4256)
                     /locus_tag="VGB88_03755"
     CDS             complement(3587..>4256)
                     /locus_tag="VGB88_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9646586.1"
                     /translation="FRIGAAVSGAVLGEREDVRAAWPGVVEAHELIGSTQIQGRASLG
                     GNLCNASPAADSVPAMVAAAARCTIAGPNGRREVAVGEVGTGPGHTSLGRGEFVVDIR
                     LPRRPPRSSDAYLRLIPRTEMDIAVVGAGVSLTIDEGGTCTDARVALGAVAPTVVLVP
                     EAARALIGTRLGDNALAALDAAARAACRPIDDKRGTVEYRTTVAGVLARRAAKIALER
                     ARSH"
BASE COUNT          584 a         1514 c         1504 g          654 t
ORIGIN      
        1 aagggctatc atgggcccgt cgacatcatt ccctgtcgcg actcggtatt gaccgaaagg
       61 gcgaaggcga tcggctcgtt cgcgcatacc attctggaag agggggcagt cgtctatgag
      121 cgggcgtgaa gccggttcgg acgctgttca gtgggaagag gcgcggcgct ggtttgccca
      181 ggccgacgac gatattcgcg tcgcatgggc gtgccttgcg gcggcaccgc caattgtcgg
      241 ggcggcggct ttgcactgtc agcaggcagc cgaaaagacg cttaaagggc tgctcatcgc
      301 cgctaggcaa aagaccggga agactcacaa tctcggcgaa ttgacgaccg ccgtggcggc
      361 ccaatatgcg ccttttcgcg atgacctcga ctggcttcgc gagctgaccg agtggtacct
      421 cgggatgcgt tatcccgaca tcgagattga gcgaccgccc gccgccgcgg cggtgagcac
      481 cgcgcttgaa agtgtcagcc gactcctcga gcgcgcacgc tcgctcgatc cgacgaggac
      541 ggccacgccg aagtaacgtc gaccgtgacc gcccgcgggc ggcctatccg ccgccgatct
      601 tgggcaggat gaacacctcg ctgtcggcgg ccaccggctc gagatagggg tcctggaaga
      661 tctcgccgtc gatcgcgatc gccatgccct gctcgatcgc ggcgccgagg ccggggtggc
      721 gggcctcgag cgcgcgcacg atcgcgcgca cggtggcgcc cgccacctcg atctcggtct
      781 cgccgccggc gaactgctgg ccgacgctgg ccggcagcac caccttggcc atcgcctcag
      841 tgcccgttgc ggagcgcggc cagcaccttg ggcggcgaca tcggcaggtc gtggagccgg
      901 accccggtcg cggcctcgat ggcgttcgcc accgccgcca tcggcgggat gatcggcacc
      961 tcgccgacgc cgcgcacgcc gaacgggtgg ttggggttcg gcacctcgac cagcacggtg
     1021 tcgatcatcg gcaggtccga ggccaccggc acgcggtaat cgaggaagcc cgggttctgc
     1081 atccggccgt cctcgccgta gatgtactcc tcgttcagcg cccagccgat gccctgcgcg
     1141 gcgccgccct ggagctggcc ctcgacatag ccgggatgga tggcgcggcc gacgtcctgg
     1201 atcgcggtgt agcgcaggat ggtgacccgc ccggtctcgc ggtcgacctc gacgtcgcag
     1261 atatgggtgc cgaagccggg cccggcgcct ttcacgttct cggtcgcctg gccgacgatc
     1321 gggccgccgg tgcgcgccga cttggccgcg atctcagcca gcgacaaggg cttgaacttg
     1381 ccggcgttgg gccccgcggg gtgcgcggcg ccgtcggccc actcgaccgc gtccgggtcg
     1441 atctcccagg tcttcgccgc ccgcgccttg agctcggcca ccaccttgcg cgtcgcgtcc
     1501 acggcgacct tgccgctggc gaaggtcacc cggctgccgc cggtaaggaa gttgaagccg
     1561 accgaggcgg tgtcggcgac caccgggcgc accttggtca cgtcgacgcc gagcacctcg
     1621 gcgaccatgt tcgccatcgc cgcgcgcgag ccgccgatat cggggttgcc ctcgaccacg
     1681 gtgacggtgc cgtcctcgtt gacgttgacc gtcgcggccg actcgccgcc gatgttgaac
     1741 cagaagcccg aggcgacgcc gcgcccctgg ttgggcccga gcggcgccgc gtaatgcggg
     1801 tggctgcgcg cggcctccaa ggtctcgatg aatccgatcg ggccgaactt cgggccgtag
     1861 gccgccttgg tgccttcgtg cgcgccgttc ttctggcgca ggtcgagcgg gtccatgccg
     1921 agcttcttgg ccagctcgtc gagcgcgctc tcgaccccga atgccgagat cggcgcgccc
     1981 ggcgcgcgat aggccgcgac cttgggccgg ttgacgacca cgtcgtagcc gaccacgcgc
     2041 acgttctcca ggtcgtaggg cgcgaacgcg gtcatgcagc cggggccgat cggcgagccc
     2101 gggaacgcgc cggcctggta catcagggtc gcctcggcgg cggtgatcct gccgtccctg
     2161 gtggcgccga tcttgatctt gccggcggcg cccgaggtcg ggcccgaggc caggaacacc
     2221 tcctcgcggc tcatcatcat cttcaccggc cggcccgacc tgcgcgccag cagcaccgcc
     2281 agcggctcga ggtagaccac ggtcttgccg ccgaagccgc cgccgatctc ggccggggtg
     2341 acccggatgt cggcgacatc catgccgagc aggcgggcga cgtaggagcg gaccatgaac
     2401 tggccctggc tcgagcacca gacctgcacc tggccgtcct cggaggcgtc ggcgaccacc
     2461 gcgtgcggct cgatgtagcc ctggtggacc ggcttggtgg tgaagtcgag ctcgaccacc
     2521 acctcggcct tcttgaaccc ggccgcgacg tcgccgagcg cgaactcgac ccgcttggcg
     2581 acgttcgagg gctggtcggg cttgggcgag acgccgtcgg tgtagaggtc gtcgtgcaac
     2641 agcggcgcat ccttggccat cgccgcgacc gggtcgatga cgtgcgccag cacctcatat
     2701 ttcacctcga tcagggacag cgcctcgcgc gcgatcgcgg acgtggtcgc ggcgacggcg
     2761 gcaagcgcgt ggccctggta gagcaccttg tcgcgcgcca tcacgttgcg cgccatgtcg
     2821 tagaagttga cctgcgcctc gcccgcggcc tcccatttcg gcgccggcgg cggcggcagg
     2881 tcggcgccgg tgatcaccgc cttgacgccg ggcagcgcct tcgccttggc ggtgtcgatc
     2941 gagatgatcc gggcatgggc gtgcgggctg cgcagcacct tgccccacag catgccgggc
     3001 aggcgcaggt cggcgccgaa cttggcccgg ccggtcacct tgtcgggccc gtcggggcgg
     3061 atggtgcgct tgccgaccca cttgtagccg ttgctcacgt cattcatggc tcatgcccct
     3121 ctcatctcgg cggccgtatc catcacggcg cggacgatct tgtcgtagcc ggtgcaccgg
     3181 cacaggttgc cggccaacca gtagcggacc tcggtctcgg tcggatcggg gttgcggtcg
     3241 agcagcgcct tggccgcgat caggaacccc ggcgtgcaga agccgcactg cagcgccgcc
     3301 tcctcgagga acttctgctg cagcggatgc agatgttcgc cccgggccat gccctcgatg
     3361 gtctcgaccg tgcggccctc ggcctcgacg ccgagcacca ggcaagcgca caccaggcgg
     3421 ccgtcgaaaa tgacgctgca cgcgccgcag tcgccggagg cgcagccctc cttgctgccg
     3481 gtcaggccga gctcgtcgcg gagtgcgtcg agcaaggtct gctctgtgtc gcacaggaac
     3541 tcgcagtcat cgccgttgac ggtggtggtg acatggtgtc tggccatcag tggctccttg
     3601 cgcgttcgag agcgatcttg gcggcgcggc gcgccagcac gccggccacc gtggtgcggt
     3661 attcgaccgt gccgcgcttg tcgtcgatcg gccggcatgc cgcgcgcgcg gcggcgtcga
     3721 gcgcggcgag cgcattatcg ccgaggcggg tgccgatcag cgcccgcgcg gcctcgggca
     3781 ccaggaccac ggtcggcgcc accgcgccga gggcgacccg ggcgtcggtg caggtgccgc
     3841 cctcgtcgat ggtcaggctg accccggccc cgaccacggc gatgtccatc tcggtgcgcg
     3901 ggatgaggcg cagataggcg tcgctcgagc gcggcggccg cctgggcaga cggatgtcga
     3961 ccacgaactc gccgcgcccg agcgaggtgt gcccgggccc ggtcccgacc tccccgaccg
     4021 caacctcgcg ccgcccgttc ggcccggcga tggtgcagcg cgccgcggcg gcgaccatgg
     4081 cgggcacgct gtcggcggcc ggcgaggcgt tgcacaggtt gccgccgagc gaggcacgac
     4141 cctggatctg ggtcgagccg atcagctcgt gcgcctcgac cacgcccggc caggccgcgc
     4201 gcacgtcctc gcgctcgccg agcacggcgc cgctcaccgc cgcgccgatg cggaag
//
LOCUS       DASWHU010000106         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3367579, whole genome shotgun sequence.
ACCESSION   DASWHU010000106 DASWHU010000000
VERSION     DASWHU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367579"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1774)
                     /locus_tag="VGB88_03760"
     CDS             complement(62..1774)
                     /locus_tag="VGB88_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9646587.1"
                     /translation="MTSPSDSGPAGRQGHPGRGRGKGRYLARGRPVEPAALDQVRALL
                     GERPRRADLLIEHLHLIQDRFGHLSAAHLAALAEEMRLAQAEVYEVATFYAHFDVVRE
                     GEAPPPAVTVRVCDGLPCALAGAEALLADLARALGAEARVVRAPCVGRCECAPVAVAG
                     HNPLEHATAEAVLAAVAARDLAAPMPAAPDLASYRADGGYQLLEACLAGRLSAEALIE
                     AVEASALCGLGGAGFPVGRKWRAVRAQPAPRLLAVNADEGEPGTFKDRYCLETAPHRV
                     LEGMLVAAWAVGAEAAYIYLRDEYPGLRALLEAALAEVAAAGLDRGLRIELRRGAGAY
                     ICGEESAMLESIEGRRGLPRHKPPFPSEVGLFARPTLIQNVETLYWLPEILDRGPDWF
                     AGEGLNGAKGLRLFSVSGRVREAGVKRAPAGITARDLIDEHCGGIAPGHAFKAYLPGG
                     ASGGILPASLADLPLDFGTLDAHGCFVGSGAVIVLGEADSIAAAVLNLMRFFEDESCG
                     QCTPCRLGTEKAAKLLAREAWDAPLLEELCRVMADASICGLGQAAPNPLRSALRHFRA
                     ELPA"
     gene            complement(1852..>2240)
                     /locus_tag="VGB88_03765"
     CDS             complement(1852..>2240)
                     /locus_tag="VGB88_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646588.1"
                     /translation="RLIGSLLALVMSAAPLAARAQGMAGCRDSFQYVLDGAREQWPDV
                     SPFLLSGEHVRYFMLAYNAEVEPDQRVLADRIVVVPLPPDLSSTWWYFGFDADCLSFY
                     AELTAHKGEHLIESGYAMLNPEQRLQ"
BASE COUNT          310 a          844 c          819 g          267 t
ORIGIN      
        1 ggccgggctg cgacctcgcg cccgtcgagg ctgaaggtga tcgcgtccgc gccgttgccg
       61 ctcatgccgg cagctccgca cggaaatggc gcaacgcgga cctgagcggg ttcggcgccg
      121 cctggccgag gccgcagatc gaggcgtcgg ccatcacccg gcacagctcc tcgagcaagg
      181 gtgcgtccca ggcctcgcgc gccagcagct tcgccgcctt ctcggtgccg agccggcacg
      241 gcgtgcactg gccgcagctc tcgtcctcga agaagcgcat caggttgagc accgcggcgg
      301 cgatcgaatc ggcctcgccg agcacgatca ccgcgcccga gccgacgaag cagccatggg
      361 cgtcgagcgt gccgaagtcg agcggcaggt cggccaagga cgcgggcagg atgccgcccg
      421 aggcgccgcc cggcagatac gccttgaagg cgtggccggg ggcgatgccg ccgcaatgct
      481 cgtcgatcag gtcgcgcgcg gtgatgccgg caggcgcccg cttgaccccc gcctcgcgca
      541 cccgcccgga caccgagaac aggcgcagtc ccttggcgcc gttgagccct tcgccggcga
      601 accagtcggg gccgcggtcg aggatctcag gcagccagta cagggtctcg acgttctgga
      661 tcagggtcgg gcgggcgaac aggccgacct cggacgggaa cggcggcttg tggcgcggca
      721 gtccgcggcg gccctcgatg ctttccagca tcgccgactc ctcgccgcag atataggcgc
      781 cggcgccgcg gcggagctcg atgcgcaggc cgcggtcgag gccggcggcg gcgacctcgg
      841 cgagcgccgc ctcgagcagc gcgcgcagcc ccggatactc gtcgcgcaga tagatgtagg
      901 ccgcctccgc gccgaccgcc caggccgcca ccagcatgcc ctcgagcacg cggtgcgggg
      961 cggtttccag gcaatagcgg tccttgaagg tgcccggctc gccctcgtcg gcgttgaccg
     1021 cgagcagccg cggcgccggc tgggccctga ccgcgcgcca cttgcgcccg accggaaagc
     1081 cggcgccgcc gagcccgcag agcgcgctcg cctccaccgc ctcgatcagc gcctccgctg
     1141 acagccggcc ggcgaggcac gcctcgagca gttggtagcc gccgtcggcg cggtaggacg
     1201 cgagatccgg ggcggccggc atgggcgcgg cgaggtcgcg cgcggcgacg gccgccaaga
     1261 ccgcctcggc agttgcgtgc tcgagcgggt tgtggccggc caccgccacc ggcgcgcact
     1321 cgcagcgccc gacgcacggc gcgcgcacca cccgcgcctc ggccccgagt gcgcgcgcga
     1381 ggtcggcgag cagcgcctcg gcgccggcca gcgcgcaggg caagccgtcg cagacgcgca
     1441 ccgtcaccgc gggcggcggc gcctcgccct cgcgcaccac gtcgaagtgg gcgtagaacg
     1501 tcgccacctc gtagacctcg gcctgcgcca ggcgcatctc ctcggcgagc gcggcgaggt
     1561 gggcggcgct gaggtggccg aagcggtcct ggatcaggtg caggtgctcg atcagcaggt
     1621 cggcgcgccg cggccgctcg ccgagcagcg cgcgcacctg gtcgagggcc gcaggctcga
     1681 ccgggcggcc gcgggcgagg tagcgcccct tgccccggcc cctgcccggg tggccctgcc
     1741 ggcccgccgg gccgctgtcg gatggcgacg tcatgtcacc gcacgcggta gcaccgctga
     1801 ccggccgcgt ccagggtggc cggcgccggc gcccgcgcgg cgagcttcgc cttattgcaa
     1861 ccgctgctcc gggttgagca tcgcatagcc gctctcgatc aggtgctccc ccttgtgcgc
     1921 ggtcagctcc gcatagaagc tcaggcagtc ggcgtcgaag ccgaaatacc accaggtgct
     1981 cgacaggtcc ggcggcagcg gcaccaccac gatgcggtcg gccaggaccc gctggtccgg
     2041 ctcgacctcg gcgttgtagg ccagcatgaa atagcggacg tgctcgcccg acagcaggaa
     2101 cgggctcacg tccggccatt gctcgcgcgc gccgtcgagc acgtactgaa agctgtcccg
     2161 gcatcccgcc atgccttgcg cacgagctgc gagcggcgcc gccgacatga cgagcgcgag
     2221 gagactcccg atcagcctac
//
LOCUS       DASWHU010000107         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1521042, whole genome shotgun sequence.
ACCESSION   DASWHU010000107 DASWHU010000000
VERSION     DASWHU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521042"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /gene="argC"
                     /locus_tag="VGB88_03770"
     CDS             complement(<1..738)
                     /gene="argC"
                     /locus_tag="VGB88_03770"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9646589.1"
                     /translation="MAKTAETIRVAVLGASGYTGAETLRLAVRHPAMRIVALTADRRA
                     GADIDGVFPHLGGLGLPRLSRIEDVAWDGVDAAFCCLPHATTQEVVAGLPERLKVIDL
                     SADFRLRDAAVYAEWYGHEHRAPALQAQAVYGLTEWARPALGQARLVACPGCYPTSAL
                     LPLIPLVRDGVVEAGDIIIDAKSGVSGAGRGLKEGSLYAEVAEGVHAYGIAAHRHAPE
                     IDQELSAAAGRALTVSFTPHLMPMNRGL"
     gene            complement(832..1317)
                     /gene="rpsI"
                     /locus_tag="VGB88_03775"
     CDS             complement(832..1317)
                     /gene="rpsI"
                     /locus_tag="VGB88_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9646590.1"
                     /translation="MAEEKRSLESLKQLVETPAEAEAEAAPVPEIDRHGRAYATGKRK
                     NAVARVWVKRGNGKVTVNGRGAETYFARPVLRMILNQPFEAAARTGQYDVLCTVSGGG
                     LSGQAGAVRHGISRALVKFEPALRAPLKQGGFLTRDARVVERKKYGKPKARKSFQFSK
                     R"
     gene            complement(1320..1784)
                     /gene="rplM"
                     /locus_tag="VGB88_03780"
     CDS             complement(1320..1784)
                     /gene="rplM"
                     /locus_tag="VGB88_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9646591.1"
                     /translation="MKTYSAKPAEVRQAWCLIDAEGVVLGRLAVLVANRLRGKHKPTY
                     TPHIDCGDHIVVVNADKVRLTGAKREDKLFRWHTGYPGGLKSRSYGQILESGEPERVV
                     RLAVERMVPRGPLGRAQLRKLHVYAGPSHPHQAQAPATLDLAAMSPKNKRSA"
     gene            2041..>2469
                     /locus_tag="VGB88_03785"
     CDS             2041..>2469
                     /locus_tag="VGB88_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9646592.1"
                     /translation="MAAIPAEQTAQPLVLRADTAEGITTLTLNRPEARNALSVALMSA
                     LQDQLDAVRAERAVKVVVIAANGPAFCAGHDLKEMRANPGRQTYEALFAQCSRLMVSI
                     THLPKPVIARVHATATAAGCQLVATCDLAVASSAARFATPG"
BASE COUNT          322 a          926 c          852 g          369 t
ORIGIN      
        1 caggccgcgg ttcatcggca tcaggtgcgg cgtgaagctg accgtgagcg cgcgcccggc
       61 ggcggcgctc agctcctggt cgatctcggg cgcgtgccgg tgggcagcga tgccgtaggc
      121 gtggacgcct tcggcgacct cggcatagag cgagccctcc ttgagcccgc gcccggcgcc
      181 cgacaccccc gacttcgcgt cgatgatgat gtcgcccgcc tcgaccacgc catccctgac
      241 cagcgggatc agcggcagca gcgccgaggt cggatagcag ccggggcacg ccaccagccg
      301 ggcttggccg agcgccggcc gcgcccattc ggtgagcccg tagaccgctt gcgcctggag
      361 cgccggcgcg cggtgctcgt ggccgtacca ctcggcgtag accgctgcgt cgcgcaagcg
      421 gaagtccgcc gacaggtcga tcaccttgag gcgctcgggc aggcccgcca ccacctcctg
      481 ggtggtggcg tgcggcaggc agcagaaggc ggcgtcgacc ccgtcccagg cgacgtcctc
      541 gatccgcgac agcctgggca ggccgaggcc gccgagatgg gggaacacgc cgtcgatgtc
      601 ggcgcccgcg cgccggtcgg cggtgagcgc gacgatgcgc atcgccggat ggcgcacggc
      661 gagcctgagc gtctccgcgc ccgtgtagcc gctcgcgccc agcaccgcga cccggatcgt
      721 ctcagccgtc ttggccatgt ccctgcctcg tcactctatc ctataacgaa aagggccggt
      781 cctcgaccag ccctcccgcc tgctgccgat ttgccgccgg ggtgccggcg ctcaccgctt
      841 ggagaactgg aagctcttgc gcgccttggg cttgccgtac ttctttcgct ccaccacgcg
      901 cgcgtcgcgg gttaggaacc cgccctgctt cagcggtgcg cgcagggccg gctcgaactt
      961 gaccagcgcg cggctgatgc cgtggcgcac cgcgcccgcc tggcccgaca gcccgccgcc
     1021 agagaccgtg cacagcacgt cgtactggcc ggtgcgcgcc gccgcctcga acggctggtt
     1081 gaggatcatg cgcagcaccg ggcgggcgaa gtaggtttcc gccccgcggc cgttgaccgt
     1141 gaccttgccg ttgccgcgct tgacccacac ccgcgcgacc gcgttcttgc gcttgccggt
     1201 ggcgtaggcg cgcccgtggc ggtcgatctc gggcacgggc gcggcctcgg cctccgcctc
     1261 cgccggcgtc tcgaccagct gcttgaggga ctcgaggctg cgtttttcct cggccatgct
     1321 tacgcgctcc gcttgttctt cgggctcatc gccgcaaggt cgagcgtcgc cggggcttgc
     1381 gcctggtgcg ggtgcgaggg cccggcatag acgtgcagct tgcgcagctg ggcgcgcccc
     1441 agcggcccgc gcgggaccat ccgctcgacc gccaggcgca ccacccgctc cggctcgccg
     1501 ctctcgagga tctggccgta gctgcggctc ttgagcccgc ccggatagcc ggtgtgccag
     1561 cggaacagct tatcctcacg cttggcgccg gtcagccgca ccttgtccgc gttgaccacg
     1621 acgatgtggt cgccgcagtc gatgtgcggg gtgtaggtcg gcttgtgctt gccgcgcagg
     1681 cggttggcga ccagcaccgc gagccgcccc agcaccacgc cctcggcgtc gatcaggcac
     1741 caggcctgcc tcacctcggc cggcttggcg gaataggtct tcatcgcaac cccgttgcgg
     1801 cccgcgaccg ctgctccgcc ggcggacccg gcgtgcggaa ggcgcgcagt atgcgcacgc
     1861 cgagccggct gtcaacgggg atttgcgccg cgatcgcaaa ggattagcgt tgtggtatca
     1921 taataccgca aggtccggcc cacggccggc gcaacgcggc cgggcgcatt tcgcccctgc
     1981 caaaccatgg gcgtgacccg tatactccgc gctccgcctg tgcgacacgg gagagacgcc
     2041 atggccgcca ttccggccga gcagaccgcc cagccgctgg tgctgcgcgc ggataccgcc
     2101 gagggcatca ccacgctcac cctcaaccgg cccgaggcgc gcaacgcgct gtcggtggcg
     2161 ttgatgtcgg cgctccagga ccagctcgac gcggtccgcg ccgagcgcgc ggtcaaggtg
     2221 gtggtaatcg cggccaacgg gccggcgttc tgcgccggcc acgacctgaa ggagatgcgc
     2281 gccaatcccg gccggcagac ctatgaggcg ctgttcgccc agtgcagtcg gctgatggtc
     2341 tcgatcaccc acctgcccaa gccggtgatc gccagggtcc acgcgaccgc caccgccgcc
     2401 ggctgccagc tggtcgcgac ctgcgacctc gcggtcgcct cgagcgccgc gcgcttcgcc
     2461 accccgggg
//
LOCUS       DASWHU010000108         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2629975, whole genome shotgun sequence.
ACCESSION   DASWHU010000108 DASWHU010000000
VERSION     DASWHU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629975"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGB88_03790"
     CDS             complement(<1..256)
                     /locus_tag="VGB88_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX9646593.1"
                     /translation="MEARSRFHGDLQALRGRVVAMAALARSMLTDGVASLVALDTAKA
                     QTVLARKDDLAAMDDEIEEEALLLVARQQPMARDMREIAAA"
     gene            complement(256..1065)
                     /gene="pstB"
                     /locus_tag="VGB88_03795"
     CDS             complement(256..1065)
                     /gene="pstB"
                     /locus_tag="VGB88_03795"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9646594.1"
                     /translation="MAIAALDEAGAPQPEPAAAAKITTRGVNVFYGDKQALFDVDIDI
                     NRNQVTALIGPSGCGKSTFLRCLNRMNDIIDGCRVEGEIRIDGEDIYDRALDVVQLRS
                     RVGMVFQKPNPFPKSIYENVAYAPRIHGLAQSRAELDEIVEQSLRRAGLWDEVKDRLD
                     QSGTGISGGQQQRLCIARAIAINPEVVLMDEPCSALDPIATAKIEELMDELRTQYTII
                     IVTHNMQQAARVSDRTAFFYLGELVEFGPTDQMFEAPTEQRTEAYITGRFG"
     gene            complement(1068..2345)
                     /gene="pstA"
                     /locus_tag="VGB88_03800"
     CDS             complement(1068..2345)
                     /gene="pstA"
                     /locus_tag="VGB88_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX9646595.1"
                     /translation="MDQAPGGRRAGASVARRLRRRHAANLRLQVYGIAAIAFAIALLG
                     VLFVSLLWTGASSFVQTHVTFAVTVPAEAVDPAAPAKANWRGFVRDSFAALFPEVTGR
                     AAQRALLDIVSNGAQFVVRDRVVADPGLIGTSFEITVPVADAFDQFHKGVIPRDVPEA
                     QRKVKDGQIAWWDALVAAGAVSMPFNWALFFATDSRFPELAGLAGALSGSFYALLVCF
                     LISFPTGIAAAIYLEEFAPKNRWTDLIEVNINNLAAVPSVVFGLLGLAVFLNFFGLPR
                     SAPLVGGMVLALMTLPTIIITGRAALTAVPPSIREAALGVGASAHQVVTHHVLPLAMP
                     GVLTGTIIGLAQALGETAPLLLIGMNAFITAEPGGLLDPATALPTQIFIWADSPERGF
                     VARTSAAILVLIGFLVVMNATAVLLRRRFERRW"
     gene            complement(2359..3738)
                     /gene="pstC"
                     /locus_tag="VGB88_03805"
     CDS             complement(2359..3738)
                     /gene="pstC"
                     /locus_tag="VGB88_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9646596.1"
                     /translation="MFTTTLLCILGLAAFGYLAARLRARRLAAAGGVRLHSLPGYHGA
                     FVALWVGIPSIVLVLIWVVLQGPVIDRLILDSMPDALTADVGSGRQQLLVSEVKSVAA
                     GRIFTEPSAAVLAAADRYNQWQGLARWAMFAAALTVALLALVWARARLAPAFRARHGV
                     ERILNGVMIVCSLIAILTTLGIVLSLLFEALRFFDRVPWYEFFFGLNWEPQMPIREDQ
                     VAGLGAFGAVPVFTGTLLAAALAMLVAVPVGLLSAVYMAEYATARFRATVKPVLEILA
                     GIPTVVYGFFAVLTVAPALRQFGAALGLDIAPNSAFAAGAVMGIMLIPFVSSLSDDAL
                     AAVPWAMREGAYGLGATKAETVVSVLLPAALPGIVGGILLALSRAIGETMIVVMAAGL
                     IATLTANPFEAVTTVTVQIVTLLIGDTEFDSPKTLAAFALGLVLFAITLALNVIALRV
                     VQKYREKYD"
     gene            complement(3818..>4141)
                     /locus_tag="VGB88_03810"
     CDS             complement(3818..>4141)
                     /locus_tag="VGB88_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9646597.1"
                     /translation="TALGIFGYSFLYENLDTLKAVKVAGIEPNFDTIADNSYPIARPI
                     FFYVKNAHRGVIPGLNEFLEEYVSDAAMAPGGYLSERGLVPLSDAMRREVQDAVLGAK
                     TYSMK"
BASE COUNT          675 a         1421 c         1480 g          565 t
ORIGIN      
        1 gggctgccgc gatctcgcgc atgtcgcgcg ccatcggctg ctgccgcgcc acgaggagca
       61 acgcctcctc ctcgatctcg tcgtccatgg ccgccaggtc gtccttgcgc gcgagaaccg
      121 tctgggcctt ggccgtgtcc aacgccacga gggacgctac accgtccgtg agcatgctgc
      181 gggccagggc ggccatggcc acgacgcggc cccgcaacgc ctgcaagtcc ccgtggaacc
      241 gactgcgtgc ctccatcagc cgaaccttcc cgtgatgtag gcttccgtgc gctgctcggt
      301 cggggcctcg aacatctggt ccgtgggtcc gaactcgacg agttcgccga ggtagaagaa
      361 ggcggtgcgg tccgagaccc gcgccgcctg ctgcatgttg tgggtgacga taatgatggt
      421 gtactgggtg cgcagctcgt ccatcagctc ctcgatcttg gcggtcgcga tcgggtccag
      481 cgccgagcac ggctcgtcca tcagcaccac ctcggggttg atggcgatcg cgcgcgcgat
      541 gcacagccgc tgctgctggc cgcccgagat gccggtgccc gactggtcga gccggtcctt
      601 gacctcgtcc cacagcccgg cgcggcgcag gctctgctcg acgatctcgt cgagctcggc
      661 gcggctctgc gcgagcccgt ggatgcgcgg cgcataggcg acattctcgt agatggattt
      721 ggggaacggg ttgggctttt gaaagaccat gccgacgcgg ctcctgagct ggaccacgtc
      781 gagcgcgcgg tcgtagatgt cctcgccgtc gatgcggatc tcgccctcga cgcggcagcc
      841 gtcgatgatg tcgttcatcc ggttgaggca gcggagaaac gtcgacttgc cgcagcccga
      901 ggggccgatc agggcggtca cctggttgcg gttgatgtcg atatcgacgt cgaacagcgc
      961 ctgcttgtcg ccgtaaaaga cgttgacccc gcgggtggtg atcttggccg ccgccgccgg
     1021 ctcgggctgg ggggcgccgg cttcgtccag tgccgcgatc gccatctcta ccagcgcctc
     1081 tcgaacctgc gccgcagcaa caccgcggtc gcgttcatga ccaccaggaa accgatcagc
     1141 accaggatcg cggccgaggt gcgggcgacg aagccgcgct ccgggctgtc cgcccagatg
     1201 aagatctggg tcggcagcgc cgtcgccggg tccagcaggc cgcccggctc ggcggtgatg
     1261 aacgcgttca tgccgatcag cagcagcggc gcggtctcgc ccagcgcctg ggcgaggccg
     1321 atgatggtgc cggtgaggac gccgggcatg gccagcggca gcacgtgatg ggtgaccacc
     1381 tgatgcgcgg acgcgccgac ccccagcgcc gcctcgcgga tcgagggcgg caccgccgtc
     1441 agcgccgcgc gcccggtgat gatgatggtc ggcagggtca tcagcgccag caccatgccc
     1501 ccgaccagcg gcgccgagcg cggcaggccg aagaagttga ggaacacggc caagcccagc
     1561 aggccgaaca ccaccgatgg caccgccgcc aggttgttga tgttgacctc gatcaggtcg
     1621 gtccagcggt tcttgggcgc gaactcctcg aggtagatgg cggcggcgat gccggtcggg
     1681 aagctgatca ggaagcacac cagaagcgcg tagaacgagc ccgagagcgc gccggcgagg
     1741 ccggcgagct cggggaagcg gctgtcggtg gcgaagaata gcgcccagtt gaacggcatg
     1801 ctcacggcgc cggcggcgac cagggcgtcc caccaggcga tctggccgtc cttgaccttg
     1861 cgctgcgcct cgggcacgtc gcgcgggatc acgcccttgt ggaactggtc gaaggcgtcc
     1921 gcgaccggca ccgtgatctc gaagctggtg ccgatcagcc cggggtcggc gaccacccgg
     1981 tcgcggacca cgaactgggc gccgttcgag acgatgtcga gcagcgcgcg ctgcgcggcc
     2041 cgcccggtga cctcggggaa cagggcagcg aagctgtcgc gcacgaagcc gcgccagttg
     2101 gccttcgccg gcgcggccgg gtcgaccgcc tccgccggca ccgtgaccgc gaaggtgaca
     2161 tgggtctgca cgaacgacga cgccccggtc cacagcagcg acacgaacag cacgccgagc
     2221 agcgcgatgg cgaaggcgat cgcggcgatg ccgtagacct gcaggcgcag attcgcggcg
     2281 tggcgccggc gcaggcggcg ggccacggat gcgccggcgc gccggccgcc cggcgcctgg
     2341 tccatcgccg aggtcgcgct agtcatactt ttcccgatac ttctgcacga cgcgcaaggc
     2401 gatcacgttg agcgccagcg tgatcgcgaa cagcaccagg ccgagggcga aggcggccaa
     2461 ggtctttggg ctgtcgaact cggtgtcgcc gatcaagagg gtaacgatct gcacggtcac
     2521 cgtggtcacc gcctcgaatg ggttggcggt caaggtcgcg atcagcccgg cggccatgac
     2581 cacgatcatg gtctcgccga tcgcccggct gagcgcgagc aggatgccgc cgacgattcc
     2641 cggcagcgcc gccggcagca gcaccgagac caccgtctcg gccttggtcg cgccgaggcc
     2701 gtaggcgcct tcgcgcatcg cccacggcac cgcggccagc gcgtcgtcgg acagggacga
     2761 gacgaacggg atcagcatga tgcccatcac cgcgccggcg gcgaaggcgc tgttgggcgc
     2821 gatatcgagg ccgagcgcgg cgccgaactg gcgcagcgcc ggcgccacgg tcagcaccgc
     2881 gaagaagccg tagaccacgg tcgggatgcc ggccaggatc tcgagcaccg gcttgaccgt
     2941 ggcgcggaac cgcgccgtgg cgtactcggc catgtagacc gccgacagca gcccgaccgg
     3001 caccgccacc agcatcgcca gcgcggcggc cagcagcgtg ccggtgaaca ccggcaccgc
     3061 gccgaacgcg ccgaggccgg cgacctggtc ctcgcggatc ggcatctgcg gctcccagtt
     3121 gaggccgaag aagaactcgt accacggcac ccggtcgaag aagcgcagcg cctcgaacag
     3181 gagcgacagc acgatgccga gggtggtcag gatcgcgatc agcgagcaga cgatcatcac
     3241 cccgttgagg atgcgctcga cgccgtggcg ggcgcggaac gcgggcgcga ggcgcgcgcg
     3301 cgcccacacc agcgcgagca gcgcgacggt gagcgccgcg gcgaacatcg cccagcgcgc
     3361 gagcccctgc cattggttgt agcggtcggc ggcggccagc acggcggcgc tcggctcggt
     3421 gaagatgcgc ccggcggcga ccgacttgac ctcgctgacc aggagctgct ggcggccgct
     3481 gccgacatcg gcggtgagcg cgtcgggcat cgagtcgagg atcaggcgat cgatcaccgg
     3541 gccctgcagg acgacccaga tcagcaccag gacgatcgag ggaatgccca cccagagggc
     3601 gacgaacgcg ccgtgatagc cgggcagtga atgcaggcgc accccgccgg ccgccgccag
     3661 ccgccgggcg cgcagccgcg cggcgaggta gccgaacgcg gcgagcccca gaatgcacag
     3721 cagcgtggtc gtgaacatgc cgctcccgtc cgggcaggtg cgccgtcgcg gggaaccggc
     3781 ggcgtcgctg accgccgggc cccgtcacgg caccgtgtta cttcatcgag taggttttcg
     3841 cgcccagcac cgcgtcctgg acctcgcgcc gcatggcatc cgacagcggc accaggccgc
     3901 gctcgctcag atacccgccc ggcgccatcg ccgcgtcgct cacgtactcc tcgaggaact
     3961 cgttgaggcc ggggatcacg ccgcgatgcg cgttcttgac gtagaagaag atcggccgcg
     4021 cgatcgggta ggagttgtcg gcgatggtgt cgaagttggg ttcgatgccg gcgaccttga
     4081 ccgccttgag cgtgtccagg ttctcgtaca ggaacgagta gccgaagatg ccgagcgcgg
     4141 t
//
LOCUS       DASWHU010000109         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3368802, whole genome shotgun sequence.
ACCESSION   DASWHU010000109 DASWHU010000000
VERSION     DASWHU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368802"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGB88_03815"
     CDS             complement(<1..359)
                     /locus_tag="VGB88_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9646598.1"
                     /translation="MAAKGAGKAEAVVVNIAEVDWRELPDHFGGAYSKLLFGPETGRG
                     RLIDHRISSYLPRAYVKAHSHKVQEQIYHVLDGEGVIELAGARRVVRRHDVIFIPPGV
                     EHSIMNTGLDNLVFLVIT"
     gene            complement(368..2167)
                     /locus_tag="VGB88_03820"
     CDS             complement(368..2167)
                     /locus_tag="VGB88_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9646599.1"
                     /translation="MPRMKGGELIVEFLVKEQVPYVFGICGHGNVGLLDALYDARDRI
                     RLVSPRHEQTAGHMADAFFRVRHQPAATLTSCGPGSSNLIMALACALADSSAFLAITA
                     NVPTQQFNRGPFQELNRHNQADFPSVARPVVKRSFQPTRVEMLPLALDQAMTTMTTGR
                     PGPVQLDVPFNLFQEEAEVELRTCAGNHISRRGGASPEDVARATDMLLAADRPVLFIG
                     HGVTLSEAGAELTALARRLRVPVISSPNGMGCLDMADPLSLGFIGRNGAYPANQAGRH
                     ADLVVAVGARFDDRSASSWLPGYSWNFPPTKLIHVDVDVAEIGRNYAPTLGIVADART
                     FLRQVLAEVEARTLDGGGRLQAWHAEIDTWRAEWDAYTAPNFDLDTSPLRPERVVKGL
                     RKTLPEDGIITLDSGVHHNWFMQFWCARRPQTMLNTWGFSSMGFGPSGILGAKLAAPE
                     RACVSVVGDGGFTMTPHVLCTAVEYDIPAVWVVWNNFAWAAIRDIQYGLFAGREIGTG
                     FYRGDNQQPYNPDFAALARACGVEAQTVTRTADLDGALDHAIRSGRPYLLDVHVDAEV
                     RPPATGTWALPPIPPKEPVFGERWQPAPERAAE"
     gene            2301..2537
                     /locus_tag="VGB88_03825"
     CDS             2301..2537
                     /locus_tag="VGB88_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646600.1"
                     /translation="MPEPASESTPRAAVRQCAAAMIDRHGAGALGLAALSASRSLSAA
                     APEDAAFWLCVCDLIRARNLAVKAQAAALRRGVH"
     gene            2624..2998
                     /locus_tag="VGB88_03830"
     CDS             2624..2998
                     /locus_tag="VGB88_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX9646601.1"
                     /translation="MLTVYGLATCDRCRKARQWLGKARIAYRFHDLRKDGLAAKTLAR
                     WSRGLGWERLLNRRSATWRTLPEAERAPLDETRALALMARLPALVSRPVIEGSGPALL
                     LAGFGAAEERALIDWAKEHDDD"
     gene            2991..>3244
                     /locus_tag="VGB88_03835"
     CDS             2991..>3244
                     /locus_tag="VGB88_03835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX9646602.1"
                     /translation="MTEGTEKKMSLSDAEWRQRLTPEQYQVARQKGTERAFSGAYWQT
                     KTPGTYRCACCGEALFGSGDKYDSGTGWPSFTRPLDAARVA"
BASE COUNT          463 a         1121 c         1217 g          443 t
ORIGIN      
        1 gtgatgacca ggaacacgag gttgtcgagg ccggtgttca tgatcgagtg ctcgacgccg
       61 ggcgggatga agatcacgtc gtggcggcgg accacgcggc gcgcacccgc gagctcgatc
      121 acgccctcgc cgtcgagcac gtggtagatc tgctcctgga ccttgtggct gtgcgccttc
      181 acataggcgc gcggcaggta gctggagatg cggtggtcga tcagccggcc gcggccggtc
      241 tcgggcccaa acaggagctt ggaataggcg ccgccgaaat ggtcgggcag ctcccgccag
      301 tcgacctcgg cgatgttgac caccaccgcc tcggccttgc ccgcaccctt cgccgccatc
      361 tgcgcgccta ctcggccgcg cgctcgggcg cgggctgcca gcgctcgccg aacaccggct
      421 ccttgggcgg gatcggcggc agcgcccagg tgccggtcgc cggcgggcgc acctcggcgt
      481 cgacatggac gtcgagcagg tacgggcgtc ctgagcggat cgcgtggtcg agcgcgccgt
      541 cgaggtcggc ggtccgggtg acggtctggg cctcgacccc gcaggcgcgg gcgagtgcgg
      601 cgaagtccgg gttgtagggc tgctggttgt cgccgcgata gaaaccggtg ccgatctcgc
      661 gcccggcgaa caggccgtac tggatgtcgc ggatcgctgc ccaggcgaag ttgttccaca
      721 ccacccacac cgccgggatg tcgtactcga ccgcggtgca cagcacgtgc ggggtcatgg
      781 tgaagccgcc gtcgccgacc accgagacgc aggcgcgctc gggcgcggcg agcttggcgc
      841 ccaggatgcc gctcggcccg aagcccatcg aggagaagcc ccaggtattg agcatggtct
      901 gcggccggcg cgcgcaccag aactgcatga accagttgtg gtggacgccg gaatcgagcg
      961 tgatgatgcc gtcctcaggc agggtcttgc gcaggccctt gaccacccgc tcaggccgca
     1021 aggggctggt gtcgaggtcg aagttgggcg cggtgtaggc gtcccactcg gcgcgccagg
     1081 tatcgatctc cgcgtgccag gcctggagcc ggccgccgcc gtcgagggtg cgtgcctcga
     1141 cctcggccag gacctggcgc aggaaggtgc gcgcgtcggc gacgatgccg agagtcggcg
     1201 cgtagttgcg cccgatctcg gccacgtcga cgtcgacgtg gatcagcttg gtcggcggga
     1261 agttccacga gtagccgggc agccatgacg acgccgagcg gtcgtcgaag cgcgcgccca
     1321 ctgccaccac caggtcagcg tggcggcccg cctggttggc cggataggcg ccgttcctgc
     1381 cgataaagcc gagcgacagc ggatcggcca tgtcgaggca gcccatgccg ttgggcgagc
     1441 tgatcaccgg cacgcgcaag cgccgggcga gcgcggtgag ctcggcgccg gcctcggaca
     1501 gagtgacgcc gtggccgatg aacagcaccg gacggtcggc ggcgagcagc atgtcggtgg
     1561 cgcgcgcgac gtcctcgggc gaggcgccgc cccgccggct gatgtggttg ccggcgcagg
     1621 tgcgcagctc gacctcggcc tcctcctgga acaggttgaa cggcacgtcg agctgcaccg
     1681 gcccgggccg gccggtggtc atggtggtca tcgcctggtc gagagcgagc ggcagcatct
     1741 cgacccgggt cggctggaag ctgcgcttga ccaccggccg cgccaccgag ggaaaatctg
     1801 cctggttgtg gcggttgagc tcctggaacg ggccgcggtt gaactgctgg gtcggcacgt
     1861 tggcggtgat cgcaaggaac gccgaggaat cggccagcgc gcaggccagc gccatgatca
     1921 ggttggacga ccccggcccg cagctggtca aggtcgccgc cggctggtgg cgcacccgga
     1981 agaacgcgtc ggccatgtgg ccggcggtct gctcgtggcg cggcgagacc agccggatgc
     2041 ggtcgcgcgc gtcgtacagc gcatcgagca ggccgacatt gccgtggccg cagatgccga
     2101 acacgtacgg cacctgctcc ttgaccagga actcgacgat cagctcgccg cctttcatgc
     2161 gcggcatgtc gctttgccct tcctgcgaaa ccggtccgcc cccgggctgt agcagcggcc
     2221 acgcccggcc gcaagggcgg agcgggcgcg gttggcacga ttgctgctgg ggaagtggcc
     2281 gccgacccag gaggcccgtc atgcccgaac ccgcatccga gtccaccccg cgcgccgccg
     2341 tgcgccaatg cgcggcggcg atgatcgacc gccacggcgc cggggcgctc ggcctcgcgg
     2401 cgctgtccgc gagccgcagc ctcagcgccg ccgcgcccga ggacgcggcg ttctggctgt
     2461 gcgtgtgcga cctgatccgc gcccgcaacc tggcggtgaa ggcgcaggcc gccgcgctcc
     2521 gccgcggcgt ccactgagcc ccagccggcg cgcgccttga ccgcgcgccg ccgccgccgc
     2581 taaatcgcgc gtaccccggc tttcgccggc ggcgggaggg ccgatgctga ccgtctacgg
     2641 gctggctacc tgcgaccgct gccgcaaggc gcggcagtgg ctcggcaagg cgcgcatcgc
     2701 ctaccgcttc cacgacctgc gcaaggacgg gctggccgcc aagacgctcg cgcgctggag
     2761 ccgcgggctc ggctgggagc ggctgctgaa ccggcgcagc gccacctggc ggacgctgcc
     2821 ggaggccgag cgggcgccgc tcgacgagac gcgggcgctg gcgctgatgg cgcgcctgcc
     2881 ggcgttggtc tcgcggccgg tgatcgaggg cagcggcccg gcgctgctgc tggccgggtt
     2941 cggcgccgcc gaggagcggg cgctgatcga ttgggcgaag gagcacgacg atgactgagg
     3001 gcacggaaaa aaagatgtcg ctgagcgacg ccgaatggcg ccagcggctg acgcccgagc
     3061 aataccaggt cgcgcggcag aagggcaccg agcgcgcgtt ttccggcgcc tactggcaga
     3121 ccaagacccc cggcacctac cgctgcgcct gctgcggcga ggcgctgttc ggttcgggcg
     3181 acaagtacga ctcgggcacc ggctggccga gcttcacccg cccgctcgat gcggcgcgcg
     3241 tcgc
//
LOCUS       DASWHU010000110         7032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_44447, whole genome shotgun sequence.
ACCESSION   DASWHU010000110 DASWHU010000000
VERSION     DASWHU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44447"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1746
                     /locus_tag="VGB88_03840"
     CDS             49..1746
                     /locus_tag="VGB88_03840"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX9646603.1"
                     /translation="MPASQHQNPKSDIDIAQAAKMRPIIDIAREKLGIAPENLQPYGH
                     YKAKLSMDFIKSLQSRPNGKLILVSAITPTPAGEGKTTTTVGLTDALNAIGKKAMLCL
                     REPSLGPCFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMIDNHIYWG
                     NELGIDPRRIGWRRVVDMNDRALRHIAVGLGGVANGFPREDGFDITVASEVMAIFCLA
                     TSLADLRARLGAIIVGYTRARAPVRAADLGAAGAMSALLAGAFKPNLVQTLEGNPAFV
                     HGGPFANIAHGCNSVIATRTALKLADYVVTEAGFGADLGAEKFFDIKCRKAGLTPASA
                     VVVATVRALKMHGGVAREALAGENVEALRKGVANLARHVENVRRFGVPVVVSINRFTA
                     DTKAELDALFQACAELEVEAVLADHWAQGSAGAAELARAVVRSANGARGAFRLLYPDD
                     MKLWDKVRTVAQAMYGAQGIIADQRVRDQFRELDAAGFGSFPVCIAKTQFSFSTDPNL
                     KGAPSNHVVPVREVRLAGGAEFVVAICGDIMTMPGLPRRPAAQAIDLDRDGRIVGLF"
     gene            2172..5651
                     /locus_tag="VGB88_03845"
     CDS             2172..5651
                     /locus_tag="VGB88_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX9646604.1"
                     /translation="MTLAAVSLDDKYALESGRVFVTGTQALVRLPLMQRRRDAAAGLN
                     TAGFISGYRGSPLGTYDQALWQASRFLARAGVEFKPAVNEDLAATALWGTQQLNLIAP
                     SRYDGVFGIWYGKGPGVDRSGDAFRHANLAGSARHGGVLVLLGDDHTAKSSTLPHQSD
                     FAMVDAMIPVLSPAGVQEFLDLGLHGIALSRFAGSWCAFKCVTATVDSSASVYIDPHR
                     VEIATPEDFALPAGGLGIRWPDHRLDQERRVHEARLPAAQAYARANRLDRVVTDSPRA
                     RIGIVAAGKSYLDVVQALDDLGIDRAAAAALGVRLYKVALVWPLEPEGLRRFADGLEE
                     IVVIEEKRGLIEDQVKGLLYNQAGRPARVVGKRDELGEVLFPPHYELTPEGIAQALAA
                     RLAPLDEAGRLARRAAEIAERHGPALRPSSVTRVPYFCSGCPHNTSTRVPEGSRALAG
                     IGCHFLAQYMERATDTFTHMGAEGASWLGQAPFTDTGHVFVNMGDGTYFHSGSLAIRA
                     AIASGANMTYKILYNDAVAMTGGQPVDGALSVAEITAQLHGEGVRRIAVVTDQPDKYP
                     RGTGFAPGTTIHHRRDLDRVQRELRETAGVSVLVYDQVCATEKRRRRKRGRLADPPRR
                     AVINELVCEGCGDCSRASNCVSVEPLETEFGRKRRINQSTCNKDFSCVDGFCPSFVTI
                     EGGELRARADRSAVAGEDDAALAGLPEPSRPALDAPYNILVTGIGGTGVVTIGAIVGM
                     AAHLEGKGCSVLDMVGLAQKGGAVLSHIRIAAAPEALHAVSIGTGRADLLLGCDLVVA
                     AAGEALARLAPGRTRAVVNGHDTPVASFVRDPDLDFAGAANRRLVAEACGEGAAQFID
                     ATALATALFGDSIATNMFMLGLAWQQGLVPVSAQAIGRAIELNGTAAAMNRAAFAWGR
                     LAAVEPERVAGEAARLAPGAACAAEDQDLEAIIDRRADFLAGYQDAAYGVRYRALVER
                     VRAAEAARVPGTEALAEAVARSAFKLMAYKDEYEVARLHADPAFLARLRATFSGDFRI
                     VYHLAPPLLAPRDARTGHLQKRAFGPWMLTVFRLLARLRGLRGTPFDPFGYSRERRAE
                     RQLIAGYLAVVEELLAGLAPANHALAVDIAALPERIRGFGHVKAASIAESAAAQARLV
                     QAFRAPASRLAAE"
     gene            complement(5943..>7032)
                     /locus_tag="VGB88_03850"
     CDS             complement(5943..>7032)
                     /locus_tag="VGB88_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEX9646605.1"
                     /translation="TPLSTSLVNALELLQGYVAITAGGLIADGSKTEVKAGRLTLDMG
                     SRRLVGRAGFRNTINAFTGIDARWTGAGGGKLRAFFTLPVDRRPNTRAALSDNRVAFD
                     DESWSQRFWSVYYAEPELPWGDRGEVYLFGLDEYDSSRRATADRTLYTPGFRLYRPAA
                     GGAFDYQVEAIVQFGESRASTAATDVAVLDHLAHFEHFEVGYSFATHWSPRLIAQYDY
                     ASGDDDPADGRNNRFDPLFGARRFDFGPTDIYGPFARSNLSSPGLRLEVKPAPGVDGM
                     LAGRSFWLASDRDTWTGAGVRDATGASGGFLGTQVEARLRWQVVPGNLQLEAGYAHLF
                     AGSFADRAPNATGEGDTDFAYGQMALTF"
BASE COUNT         1009 a         2576 c         2420 g         1027 t
ORIGIN      
        1 ctccactcct tccgttcccg cggctctctc agattggact agtcaaccat gcccgcctcg
       61 cagcatcaga atcccaagtc cgacatcgac atcgcccagg ccgccaagat gcggccgatc
      121 atcgacattg cgcgcgagaa actcggcatc gcgccggaga acctccagcc ctatggccac
      181 tacaaggcca agctgtcgat ggatttcatc aagtcgctgc aaagccggcc gaacggcaag
      241 ctgatcctgg tttcggcgat cacgccgacg ccggcaggcg agggcaagac gacgaccacc
      301 gtcgggctca ccgacgcgct caacgcgatc ggcaagaagg cgatgctgtg cctgcgcgag
      361 ccgtcgctcg ggccgtgctt cggcgtcaaa ggcggcgccg ccggcggcgg ctatgcccag
      421 gtggtgccga tggaggacat caatctccac ttcaccggcg acttccacgc catcggcgcc
      481 gccaacaacc tgctcgcggc gatgatcgac aaccacatct actggggcaa cgagctcggc
      541 atcgacccgc gccggatcgg ctggcggcgg gtggtcgaca tgaacgaccg ggcgctgcgc
      601 catatcgcgg tcgggctcgg cggggtggcc aacggcttcc cgcgcgagga cggcttcgac
      661 atcacggtcg ccagcgaggt gatggcgatc ttctgcctgg ccaccagcct cgccgacctg
      721 cgcgcccgcc tcggcgcgat catcgtcggc tacacccgcg cgcgcgcgcc ggtgcgggcc
      781 gccgatctcg gcgccgcggg cgcgatgagc gcgctgctcg ccggtgcctt caagcccaac
      841 ctggtccaga ccctcgaagg caacccggcc ttcgtccacg gcggcccgtt cgccaacatc
      901 gcccatggct gcaactcggt gatcgccacc cgcaccgcgc tcaagctcgc cgactacgtg
      961 gtcaccgagg ccggcttcgg cgcggacctt ggcgccgaga agttcttcga catcaagtgc
     1021 cgcaaggccg ggctcacccc ggcgtctgcg gtggtggtgg cgaccgtgcg cgcgctcaag
     1081 atgcacggcg gcgtggcgcg cgaggcgctg gcgggcgaga acgtcgaggc gctcaggaag
     1141 ggcgtcgcca acctcgcccg ccacgtcgag aacgtgcgcc ggttcggggt gccggtggtg
     1201 gtgtcgatca accgcttcac cgccgacacc aaggccgagc tcgacgccct gttccaggcc
     1261 tgcgccgagc tcgaggtcga ggcggtgctc gccgaccact gggcgcaggg cagcgccggc
     1321 gcggccgagc tggcgcgcgc ggtggtgcgc agcgccaacg gcgcgcgcgg cgcgttccgc
     1381 ctgctctatc ccgacgacat gaagctgtgg gacaaggttc gcaccgtcgc ccaggcgatg
     1441 tacggcgcgc aaggaatcat cgccgaccag cgggtgcgcg accagttccg cgagctcgac
     1501 gcggccggct tcggcagctt tccggtgtgc atcgccaaga cccagttcag cttctccacc
     1561 gacccaaacc tcaagggcgc gccgagcaac cacgtggtgc cggtgcgcga ggtgcggctc
     1621 gccggcggcg ccgagttcgt ggtcgcgatc tgcggcgaca tcatgaccat gccgggcctg
     1681 ccccgccgcc ccgcggcgca agcgatcgac ctcgaccggg acgggcgcat cgtcggcctg
     1741 ttctgaccgg cccggggcgc accccgcggc cggccttcct tgtggaaaag tagtggataa
     1801 tttcgctgtc gaaataatcg ccgcgagcga gtgaagacct ctcccggccc cgattcgtac
     1861 tgccaacgca atttgttcct ggtttgtcta agcatcgaac tgtcacaaat tccggccgga
     1921 cattgcaaat cggtgttcgt tttatgaacg gcacggagcg ctctccgtca tgatccgggc
     1981 cttcaaatgt tatccccatg attcataacg caggtttaga aagcctgtgg ataacctccg
     2041 ggcgcggggc ttgtgacagc gcctgtcatg cgaccgtgac cggcgacgat tgccaagtcg
     2101 ggccatctcg gctacctgct taggcggccg gcccgcccgg ctcgccgtcg ccgcgcggaa
     2161 ggatgcccgc gatgaccctc gccgccgtca gcctggacga caagtacgcg ctcgagtcgg
     2221 gccgggtgtt cgtcaccggc acccaggcgc tggtccgcct gccgctgatg cagcgccggc
     2281 gcgacgccgc cgccggcctc aacaccgccg gcttcatctc cggctatcgc ggctcgccgc
     2341 tcggcaccta cgaccaggcg ctgtggcagg ccagccggtt cctcgcccgc gccggcgtcg
     2401 agttcaagcc ggcggtcaac gaggacctgg cggcgaccgc gctgtggggc acccagcagc
     2461 tcaacctgat cgcgccgtcg cgctacgacg gcgtgttcgg catctggtac ggcaagggcc
     2521 cgggggtcga ccgctcgggc gatgccttcc gccacgccaa cctcgccggc agcgcccgcc
     2581 acggcggggt gctggtgctg ctcggcgacg accacaccgc caagtcctcg accctcccgc
     2641 atcagagcga cttcgccatg gtcgacgcga tgatcccggt gctgagcccc gccggggtgc
     2701 aggagttcct cgacctcggc ctgcacggca tcgcgctgtc gcgatttgcc ggcagctggt
     2761 gcgcgttcaa atgcgtgacc gcgaccgtcg attcatcggc ctcggtctac atcgacccgc
     2821 accgggtgga gatcgcgacg ccggaagatt tcgcgctgcc cgcgggcggc ctcggcatcc
     2881 gctggcccga ccatcgcctc gaccaggagc ggcgcgtgca cgaggcgcgc ctgccggcgg
     2941 cccaggccta tgcgcgcgcc aaccgcctcg accgcgtggt caccgactcg ccgcgcgcgc
     3001 gcatcggcat cgtcgccgcg ggcaagtcct atctcgacgt cgtccaggcg ctcgacgacc
     3061 tcggcatcga ccgcgccgcg gcggccgcgt tgggcgtgcg cctgtacaag gtggcgctgg
     3121 tctggccgct cgagcccgag ggactcaggc gtttcgccga cggcctcgag gagatcgtcg
     3181 tcatcgagga gaagcgcggc ctgatcgagg accaggtcaa gggcctgctc tacaaccagg
     3241 cggggcggcc ggcgcgcgtg gtcggcaagc gcgacgagct gggcgaggtg ctgttcccgc
     3301 cccattacga gctcaccccc gaaggcatcg cccaggcgct cgcggcgcgc ctcgcgccgc
     3361 tcgacgaggc gggcagactc gcgcgccgcg cggcagagat cgcggagcgc cacgggcccg
     3421 cgctgcgccc ctcgagcgtg acccgcgtgc cctatttctg ctcgggctgc ccgcacaaca
     3481 cctcgacccg ggtgcccgag ggcagccgcg ccttggccgg catcggctgc cacttcctgg
     3541 cccagtacat ggagcgcgcc accgacacct tcacccacat gggcgccgag ggcgcgagct
     3601 ggctcggcca ggcgccgttc accgacaccg ggcacgtgtt cgtcaacatg ggcgacggca
     3661 cctacttcca ctccggcagt ctcgccatcc gcgccgcgat cgcctcgggc gcgaacatga
     3721 cctacaagat cctctacaac gacgcggtgg caatgaccgg cggccagccg gtcgacggcg
     3781 cgttgagcgt ggccgagatc accgcccagc tccacggcga gggcgtgcgc cggatcgcgg
     3841 tggttaccga ccagccggac aagtatccgc gcggcaccgg cttcgccccc ggcaccacca
     3901 tccaccaccg ccgcgacctc gaccgcgtcc agcgcgagct gcgcgagacg gcgggggtct
     3961 cggtgctggt ctacgaccag gtctgcgcga ccgagaagcg ccgccggcgc aagcgcgggc
     4021 gcctggccga cccgccccgg cgcgcggtca tcaacgagct ggtgtgcgag ggctgcggcg
     4081 actgctcgcg cgcctcgaac tgcgtctcgg tcgagccgct cgaaaccgag ttcgggcgca
     4141 agcgtcggat caaccagtcg acctgcaaca aggacttctc gtgcgtcgac ggcttctgcc
     4201 cgagcttcgt caccatcgag ggcggcgagc tgcgcgcccg cgcggaccgc tcggcggtcg
     4261 ccggcgagga cgacgcggcg ctcgccggcc tgcccgagcc gtcgcgcccg gcgctggatg
     4321 cgccctacaa catcctggtc accggcatcg gcggcaccgg cgtggtcacc atcggcgcga
     4381 tcgtcggcat ggccgcgcat ctcgagggca agggctgctc ggtgctcgac atggtcgggc
     4441 tcgcccagaa gggcggcgcg gtgctcagcc acatccgcat cgcggcggcg cccgaggcgc
     4501 tgcatgcggt ctcgatcggc accggccggg ccgacctgct gctcggctgc gacctggtgg
     4561 tcgcggccgc gggcgaggcg ctggcccggc tcgcgccggg gcgcacccgc gccgtggtca
     4621 acggccacga caccccggtc gcgagcttcg tgcgcgaccc cgacctcgac ttcgccggcg
     4681 ccgccaaccg ccggctggta gccgaggcct gcggcgaggg cgcggcgcag ttcatcgacg
     4741 ccaccgccct cgccaccgcc ctgttcggcg actcgatcgc gaccaacatg ttcatgctcg
     4801 ggcttgcctg gcagcagggc ctggtcccgg tctcggcgca ggcgatcggg cgcgcgatcg
     4861 agctcaacgg caccgcggcg gcgatgaacc gcgccgcctt cgcctggggc cggctcgccg
     4921 cggtcgagcc cgagcgggtg gcgggcgagg ctgcgcgcct cgcccccggc gcggcgtgcg
     4981 cagcggagga ccaggacctc gaggcgatca tcgaccggcg tgcggatttc ctcgccggct
     5041 accaggacgc cgcctacggc gtgcgctacc gggcgctggt cgagcgggtg cgcgccgccg
     5101 aggccgcgcg cgtcccgggc acggaggcgc tcgccgaggc cgtcgcccgc tcggccttca
     5161 agctgatggc ctacaaggac gagtacgagg tcgcccgcct gcacgccgac ccggccttcc
     5221 tcgcccgcct gcgcgcgacc ttcagcggcg atttccggat cgtctaccac ctggcgccgc
     5281 cgctgctagc gccgcgcgat gcgcgcaccg ggcatctgca gaagcgcgcg ttcgggccgt
     5341 ggatgctgac cgtgttccgg ctgctggcgc gcctgcgcgg cctgcgcggc acgccgttcg
     5401 acccgttcgg ctacagccgc gagcggcgcg ccgagcgcca gctgatcgcc ggctacctcg
     5461 ccgtggtcga ggagctgctg gccgggctcg cgcctgccaa ccacgcgctc gcggtcgaca
     5521 tcgccgcgct gcccgagcgg attcgcggct tcggccacgt caaggccgcg agcatagccg
     5581 agtccgcggc cgcgcaggcg cgcctggtgc aggcgttccg ggccccggcg tcgcgcctcg
     5641 ccgccgagtg aggcgaccgg ccgtcccggc ccgtgccgcg ggccagactg gtgcgccgca
     5701 gcagacttaa ggcagacctc ctccctgtct tttcaatgac ttacaatttc cgcctccgcg
     5761 gagtgtgcgg cgcagaaaaa ttattgcatc gcaaaatacg gcactttata tttgcccttg
     5821 gcactcggtg ggtcccccct cgccgatctg cccgcccttg ttgggcgttt cctccctgaa
     5881 cttgggccgt ggctgtccag ccacggcctt ttttcgtgcc gaggctcgcg ggccagccgg
     5941 cctcagaagg tcagcgccat ctggccgtag gcgaaatcgg tatcgccctc gcccgtggcg
     6001 ttgggcgcgc ggtcggcgaa ggagccggcg aacagatggg cgtagccggc ctcgagctgc
     6061 aggttgccgg gcaccacctg ccagcgcagc cgcgcctcga cctgggtgcc gagaaagccg
     6121 cccgatgcgc cggtggcatc gcgcacgccg gcccccgtcc aggtgtcgcg gtcggaggcg
     6181 agccaaaagc tgcgcccggc gagcatgccg tcgacgccgg gcgccggctt gacctcgagc
     6241 ctgagaccgg gcgagctcag attgctgcgc gcgaacgggc catagatgtc ggtcgggccg
     6301 aagtcgaagc ggcgcgcgcc gaacagcggg tcgaagcggt tgttgcggcc gtcggccggg
     6361 tcgtcgtcgc cgctcgcata gtcgtattgg gcgatcagcc gcggcgacca gtgcgtggcg
     6421 aacgaatagc cgacttcgaa atgctcgaaa tgagcgaggt ggtcgaggac ggcgacatcg
     6481 gtcgcggcgg tggacgctcg cgattcgccg aactggacga tcgcttcgac ctggtaatcg
     6541 aacgcgccgc ccgccgccgg ccgatagagc ctgaagccgg gcgtgtacag ggtccggtcg
     6601 gcggtggcgc ggcgcgagct gtcatactcg tcgaggccga ataggtagac ctcgccgcgg
     6661 tcgccccagg gcagctccgg ctcggcgtag tagacgctcc agaacctctg gctccagctt
     6721 tcgtcgtcga acgcgacgcg gttgtcgctc agcgccgccc gggtgttcgg ccggcggtcg
     6781 accggcaacg tgaagaacgc gcgcagcttg ccaccgccgg cgccggtcca gcgcgcatcg
     6841 atgccggtga atgcgttgat cgtgttgcga aacccggccc gcccaaccag ccgccggctg
     6901 cccatgtcga gggtcagccg cccggccttg acctcagtct tgctgccgtc cgcgatcagg
     6961 ccgcccgcgg tgatggcgac gtagccctga agcagctcaa gcgcattcac caggctggtg
     7021 ctgagcgggg tg
//
LOCUS       DASWHU010000111         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1523050, whole genome shotgun sequence.
ACCESSION   DASWHU010000111 DASWHU010000000
VERSION     DASWHU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523050"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VGB88_03855"
     CDS             complement(<1..851)
                     /locus_tag="VGB88_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9646606.1"
                     /translation="MAEVIETVAARVQESDLSRFVRDLLAAAGADVRSAAAVTRAVVE
                     ASARGTDTHGVRLVPIYVKAVETGQVNGSPNIAFRQTAPAAGMVDADRGFGHPASFHA
                     VEHAMALARASGIAVVAVTNSSHFGAAGVYVREAAGAGMIAIGLSHADALVAPHGGIA
                     PFFGTNPFAFACPVAGAEPVVLDMATSAIPFNRVHLCKALGKALPAEVAVDAAGAPTR
                     DPDRVSALMPLGGADYSYKGAGLAAMIDLVCAGLTGMAHGFRQPNFVATGTIPVPIGH
                     CFIVIDPS"
     gene            complement(865..1716)
                     /locus_tag="VGB88_03860"
     CDS             complement(865..1716)
                     /locus_tag="VGB88_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9646607.1"
                     /translation="MKLVRYGRRGREKPGLIDADGKLRDLSGKIADITPETLSEKGLA
                     RLARLSPRRLPLVRGRPRLGVPWTGIGKVMCIGLNYADHAKEAGQPIPAEPILFLKAN
                     SAINGPFDEVMLPRASKKGDWEVELGVVIGKLASNVSKRAALDHVAGYTIVNDVSERE
                     FQLERGPTWTKGKSCDTFGPIGPWLVTRDEIPNPQRLGMWLDVNGVRRQTGNTRTMIF
                     GCAELISYLSAMITLYPGDVISTGTPPGVGAGFKPPVFLKPGDVMALGIDGLGAQRQK
                     VVAYKKP"
     gene            complement(1784..>1910)
                     /locus_tag="VGB88_03865"
     CDS             complement(1784..>1910)
                     /locus_tag="VGB88_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9646608.1"
                     /translation="RQPMGRLARPEEVAALAVYLASDESAFMTGQAIVIDGGISI"
BASE COUNT          275 a          660 c          695 g          280 t
ORIGIN      
        1 gacgggtcga tgacgatgaa gcagtggccg atcggcaccg ggatggtccc ggtggcgacg
       61 aagttgggct ggcgaaagcc gtgcgccatg ccggtcagcc cggcgcagac gaggtcgatc
      121 atcgccgcga gccccgcgcc cttgtaggag tagtcggcgc cgcccaacgg catcagcgcg
      181 cttacccggt cgggatcgcg ggtcggcgcg cccgcagcgt cgaccgccac ctcggcgggc
      241 agcgccttgc ccagcgcctt gcacagatgc acgcggttga acgggatcgc gctggtcgcc
      301 atgtcgagca ccaccggctc cgcccccgcc accgggcagg cgaaggcaaa cgggttggtg
      361 ccgaagaacg gcgcgatgcc gccgtgcggc gcgaccagcg cgtcggcgtg ggagaggccg
      421 atcgcgatca tgccggcgcc cgccgcctcg cgcacgtaga cgccggcggc gccgaaatgg
      481 gacgagttgg tcaccgcgac cacggcaatg ccggacgcgc gcgcgagcgc catcgcatgc
      541 tcgaccgcgt gaaagctcgc cgggtggccg aagccgcggt cggcgtcgac cattccggcg
      601 gcgggcgcgg tctgccggaa cgcgatgtta gggctgccgt tgacctgccc ggtctcgacc
      661 gccttgacgt agatcggcac caggcggacg ccgtgggtgt cggtgccgcg ggccgaggcc
      721 tcgacgactg cgcgcgtgac cgccgccgcc gagcgcacat cggcgccggc ggcggcgagc
      781 aggtcgcgaa cgaagcggct gaggtcggac tcttgcaccc gcgccgccac ggtctcgatg
      841 acctcggcca tcggccgcgg ccggtcaggg cttcttgtag gcgacgacct tctgccgctg
      901 ggcgccgagc ccgtcgatgc cgagcgccat cacgtcgccg ggcttgagga agaccggcgg
      961 cttgaagccg gcaccgacgc cgggcggggt gccggtggag atgacgtcgc cgggatagag
     1021 ggtgatcatc gcgctcagat agctgatcag ctcggcgcag ccgaagatca tcgtgcgggt
     1081 gttgccggtc tggcggcgca cgccgttgac gtcgagccac atgccgagcc gctgcgggtt
     1141 gggaatctcg tcccgcgtca ccagccacgg gccgatcggg ccgaaggtgt cgcagctctt
     1201 gcccttggtc caggtgggcc cgcgctcgag ctggaactcg cgctcggaga cgtcgttgac
     1261 gatggtgtag ccggcgacgt ggtcgagcgc ggcgcgcttg gagacgttcg aggccagctt
     1321 gccgatcacc acgccgagct cgacctccca gtcgcccttc ttggatgcgc gcggcagcat
     1381 cacctcgtcg aacgggccgt tgatcgcgct gttcgccttg aggaacagga tcggctccgc
     1441 agggatcggc tggccggcct ccttggcgtg gtcggcgtag ttgagcccga tgcacatcac
     1501 cttgccgatg ccggtccacg gcacccccag ccgcggccgg cccctgacca gcggcaagcg
     1561 gcgcgggctg aggcgggcga gccgcgccag ccccttctcg gacaaggtct caggagtgat
     1621 gtcggcgatc ttgccgctca ggtcgcgcag cttgccgtcg gcgtcgatca ggcccggctt
     1681 ctcgcggccg cgccgtccat agcgtacgag cttcatgggt cctcctctgt gctccctccc
     1741 cggggaaccc gggacgaatg atgagactgc taacgactgc gtctcaaatg ctgatgccgc
     1801 cgtcgatcac gatcgcctgc ccggtcatga acgccgactc gtcggaggcg aggtagaccg
     1861 cgagcgcggc gacctcctca ggcctcgcaa ggcggcccat cggctggcgg
//
LOCUS       DASWHU010000112        18139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2268133, whole genome shotgun sequence.
ACCESSION   DASWHU010000112 DASWHU010000000
VERSION     DASWHU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18139
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268133"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGB88_03870"
     CDS             complement(<1..242)
                     /locus_tag="VGB88_03870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553632.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9646609.1"
                     /translation="MSLPGLCRDCGARLAQAAALRCPACGGPRLVRHGELNQLAIAHL
                     DCDAFYAAVEKRDNPALQDKPVIVGGGRRGVVSTAC"
     gene            complement(357..1034)
                     /locus_tag="VGB88_03875"
     CDS             complement(357..1034)
                     /locus_tag="VGB88_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646610.1"
                     /translation="MAGPPADTAAAHARIAALDLAPDRPLIVSDADEVLFVFMAAFEG
                     FLDRRGHYFDWMSFALDGNVRSRADDTALPRAAVAGLLDAFFEHHTASLEPVPGAAAA
                     LAGLSARAQILVLSNIPAAQAPIRRAALSRHGMDYPLIANTGPKGPVVEALAEAVAAP
                     VFFLDDGPRHHASVARHAPRVRRLHLIADTRLADLLGPVSEAHHAVRHWPEARALIES
                     ELAAAGY"
     gene            complement(1035..1307)
                     /locus_tag="VGB88_03880"
     CDS             complement(1035..1307)
                     /locus_tag="VGB88_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007204239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HEX9646611.1"
                     /translation="MTPEAAETIALQALAHVVGETEARERFLALSGADLATLRAEAGN
                     AAFLAGVLDFVLGDEALLLAFCDAAGLAPETPARARAALPGAAMSD"
     gene            complement(1370..1897)
                     /locus_tag="VGB88_03885"
     CDS             complement(1370..1897)
                     /locus_tag="VGB88_03885"
                     /inference="COORDINATES: protein motif:HMM:NF021602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEX9646612.1"
                     /translation="MNEQPEPPDTDDKKPTKDALPVPAEDEDAQRLKAALRNILRRAA
                     IDPDDPDAQLIISAAHSWTYRGPIPPPGMLKQYDAVIPGLADRMVGGWDDERRHRHSL
                     EDRAFEGDESRMNRAQYHALTLGLLGIVAAGVIAILDGWEAAAVVAIVAIGGPTAATA
                     LSRLVSLPRLDRNSD"
     gene            complement(2311..2988)
                     /locus_tag="VGB88_03890"
     CDS             complement(2311..2988)
                     /locus_tag="VGB88_03890"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9646613.1"
                     /translation="MGAIGELPQARQLRTGSSPRHDRRAPRTSPRNPRFRRTAVFLPA
                     VIRNRAGDIACQVLNISASGALIRIPSGAEPANAFAFRIERHPDLYAEVVRNHGDQFG
                     IAFIDRHGQVQAIIDRLLSDTASNRDLRRFPRRLVLLSGSFYLGDQLVPFSVHNLSAG
                     GAFLAAPARLGRGHEIRLRVDRFGTMPAAVVWSSRAGMGASFWDEPTAVLDRIGHLLP
                     PTPRDNL"
     gene            complement(3247..3414)
                     /gene="rpmG"
                     /locus_tag="VGB88_03895"
     CDS             complement(3247..3414)
                     /gene="rpmG"
                     /locus_tag="VGB88_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9646614.1"
                     /translation="MAKPNSLKIRLNSTADTGHFYVTKKNPRTMTGKLEIKKYDPVAR
                     KHVLYKEGKIK"
     gene            complement(3490..4668)
                     /locus_tag="VGB88_03900"
     CDS             complement(3490..4668)
                     /locus_tag="VGB88_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9646615.1"
                     /translation="MTVQEPAARPGPVRGLVAAISATTLLGTTIGLGLPLFALVLEAR
                     GTGETLIGVNSAAQFLGIMLCAPLAPGVIRRAGLVGAMNAGILGCAACLALLAAFDSF
                     AAWAVVRFAFGGAEGLLFVAGETWINAAIDDRVRGRLVAVYTTSLAAGFALGPLIITA
                     TGADGPLPFLVGALVVLSGVVPVLIGARSAPTIAGVPSGGALRLLRAMPVAAAAAALF
                     GVLDGGMVALLAVYGLSLDLEAPAAARLVTTLAVGAIVLQLPLGWLADRTDRRAAVAG
                     SAAAGTVLLLAVAAFGASPIALHVLLFAIGGLLGSFWMFAMTLLGERFRGADLAAGNV
                     GLTLAYGVGSVAGPALGGVAMELWSPHGLMAAMAAATGAFALSVEIGRRRTARPAAGA
                     "
     gene            complement(4665..6884)
                     /gene="rnr"
                     /locus_tag="VGB88_03905"
     CDS             complement(4665..6884)
                     /gene="rnr"
                     /locus_tag="VGB88_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX9646616.1"
                     /translation="MAKASGPKQRRAEGLPSREQILEFLETADHKVGKREIARAFKLK
                     GADRAALKEVLRELADAGLIVRGQRKSLAAGGALPAVCVVEVTGLDLDGDPLARPVDW
                     RGEAPPPSVTVLPALRPRTHAPGVGDRVLVRTRKIAEDLYEGRVIRVLGGRPGRLVGV
                     YRPAGDGAGGRIKPVERRARSEFFVAQGDARDARAGDLVIAETLPAHRLGLPQARVID
                     VLEGMDSPRSIGLLVINQHGIPSHFPPEAVARAEAARPLGVRGRADLRKLPLVTIDDE
                     DARDFDDAVFAEPDPDSRGGWHLVVAIADVGHYVRPGDAVDREARRRGNSVYLPDMVV
                     PMLPEALSNGLCSLRPGEDRGCVAAHLWIDGDGGLVRHRFERALMRSAARLTYRGVQA
                     AIDGRAAAEVEPLAEPVIRPLDGAFRRLLAARRKRGTLDLDLPERRVLFDDAGAVRAI
                     ETRPRYDSHRIIEEFMILANVAAAETLVARRAPCMFRIHDQPPLDKLEALRNYLSSLG
                     YRLARGRHATTAQFNHILDKARGTPEEESVALTVLRSQAQAEYSPDNIGHFGLNLRRY
                     AHFTSPIRRYADLLVHRSLIGALGLGAGALPKDADQDFAVVGAEISATERRAATAERD
                     ALDRFATRFLAERVGARFAARITGVTRFGLFVQLADTGADGLIPIRDFGEYMEHDPGR
                     HRLTGRDSGRAYALGEMLEVRLAEADLVTGTLRFELEHAAGTKTQVARGRTRAKRGR"
     gene            complement(6897..9515)
                     /gene="topA"
                     /locus_tag="VGB88_03910"
     CDS             complement(6897..9515)
                     /gene="topA"
                     /locus_tag="VGB88_03910"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9646617.1"
                     /translation="MNEKTTDTTNVVIVESPAKARTIGKYLGPGYRVVATYGHVCDLP
                     SKDGSVRPDEDFAMDYVADPKSAKQLKQITTEAKGAGRLLLATDPDREGEAISWHVLN
                     HLRAKGALEGKTAERVVFYEVTRDAVREALAHPRDIDMDLVNAQQARRALDYLVGFTL
                     SPVLWRKVPGSRSAGRVQSVALRLIVDREMEIEAFKARQYWTVDAELARASGERFSAR
                     LVTLDGAKLDKFDLADEAAATRAAQAVAASALRVTAVERKQVRRTPPPPFTTSTLQQE
                     AARKLGFTAQRTMRVAQRLYEGVDLAGETVGLISYMRTDSVTLSNEALTAARAAIERL
                     YGGTYLPESPRRYTNRAKNAQEAHEAIRPTDAARHPRELAIGEDEGRLYELIWKRTVA
                     SQMEPAVLDQVGVTIASADAKVALRATGSVVAFDGYYRLYTEGRDEPAADDEGEGRRL
                     PAVSEGEALSLGQVTPAQHFTEPPPRYSEASLVKRLEELGIGRPSTYANIITVLQDRD
                     YVRLEKKRFVPEDRGRLVTAFLESFFERYVQYDFTARLEDRLDLISNGKLDWKDVLRE
                     FWSGFSLAVDDIGHLRVRDILDKLDSFLGPHIFPANGSGRDPRACPACADGRLSLKLG
                     KFGAFVGCSNYPDCRYTRPLVVVNGGNGEGAGLSEGPRALGSAPDGAEVTLRSGPYGL
                     YVQREGAGDGDKPKRVSLPKGMDPEAVDLAMAIALLDLPREVGTHPETGAPITAGVGR
                     YGPYIKHQDSFKSLKDGDDVLTIGLNRAVALLAEAGGARRGSTRLRVLGRHPDDDREV
                     ALYSGRYGPYVRHNSVIANLPKDADPAAVTLADALPLLAEKAAKKTKGRKRAKPASTA
                     RKSTGKARAAAVTDPA"
     gene            complement(9538..10671)
                     /gene="dprA"
                     /locus_tag="VGB88_03915"
     CDS             complement(9538..10671)
                     /gene="dprA"
                     /locus_tag="VGB88_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9646618.1"
                     /translation="MHGARSGLAASERLDWLRLWRSENVGPRTFFDLLRATGSAAAAL
                     DMLPELARRGGLRRRIRACSRDDAAREADALAAIGGHLLAWGEPGFPRALAAIPDPPP
                     IISALGHLDLLDRNSVAVVGARNASGIGRRFARGLAAELGAGGLVVVSGLARGIDAEA
                     HAGALAHGTVAAQAGGIDIVYPAENQALYESIRDAGALVSEQPLGLAPQARHFPRRNR
                     IVSGLALGVVVVEASRRSGSLITARLALEQGREVFAVPGSPLDPRHGGTNLLIRQGAV
                     LTESAEDVLSSLAEMTSRRLGETPEAPAGIGISSKEISELDVTDADRERVMAALGPTP
                     LLVDELVRQCQLTAAEVMIVMLELELAGRVIRHPGGSISISDQ"
     gene            complement(10704..11357)
                     /gene="plsY"
                     /locus_tag="VGB88_03920"
     CDS             complement(10704..11357)
                     /gene="plsY"
                     /locus_tag="VGB88_03920"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX9646619.1"
                     /translation="MPDLLGGLAYTWPYYAAIPVCYLIGSIPFGLIVARVAGLGDIRR
                     IGSGNIGATNVLRTGHTALALLTLALDLGKGTAAVAAAGFYGPDFEYIAGLSVVLGHM
                     FPVWLRFQGGKGVATAFGVLFAVSWPVALAAGAGFAIVAAATRIVSVASMIAMIAAPA
                     MLWAMLALQRQETLPYWLPGLPQHVDLMAVIAVLVLVRHHANIRRLMSGKEPRIGKA"
     gene            complement(11360..12685)
                     /gene="pyrC"
                     /locus_tag="VGB88_03925"
     CDS             complement(11360..12685)
                     /gene="pyrC"
                     /locus_tag="VGB88_03925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9646620.1"
                     /translation="MSTQASAARPGRVAYVNARLLDPASGRDLRGALLTDARRIADLG
                     PGLFADGVPEGIDVVDCAGHCLAPGLVDMRAHLREPGAEHKETLATASRAAAAGGVTS
                     IACMPNTDPPIDQIALIEFIARRARETSLVKIYPVAAITRGLEGRELTEIGMLAEAGA
                     VGFSDADRAVMDSRVMLRALTYAKPFGRLVMQHPEDRALTEGGMMNDGELASRLGLAG
                     MPAEAETVIIERDLRLAARAEAPYHVAHVSTAAAIDVVRRAKARGEPVTCATAPPYFA
                     LNETAVDDYRTFAKVRPPLRSEADRAAVVAGLADGTIDAIASDHAPHDQDSKRTTFAQ
                     AAFGIIGLETMLPLALELVHAGALSLVEVLARMTAAPARLLGLEQGRLAVGAPADLVV
                     FDPDRPWRIDTSALRSKSKNAPYDERPVQGRVLRTVVGGETVFRLDGAP"
     gene            complement(12682..13653)
                     /locus_tag="VGB88_03930"
     CDS             complement(12682..13653)
                     /locus_tag="VGB88_03930"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9646621.1"
                     /translation="MTGPASNRPFARHHLLGIADMTAEEIGYLLDLAESYVALNRRVD
                     KKQTLLQGQTLINLFFESSTRTRTSFELAGKRLGADVINMQVQASSIKKGETLIDTAL
                     TLNAMRPDILVVRHNDSGAVNLLSEKVECAVINAGDGSHEHPTQALLDALTIRRRRGR
                     IDGLTVAICGDVRHSRVARSNIHLFNIMGARVRLIAPPTLLPTAAERFGVEVFHDMRA
                     GLKDCDIVMMLRLQTERMQGSYVPSIREYFHFWGLDHDKLALARPDALVMHPGPMNRG
                     VEIDSELADDLTRSVILDQVELGVAVRMACLDALARRRGPALTEAVA"
     gene            complement(13706..14170)
                     /gene="ruvX"
                     /locus_tag="VGB88_03935"
     CDS             complement(13706..14170)
                     /gene="ruvX"
                     /locus_tag="VGB88_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9646622.1"
                     /translation="MTTDDLRAFAALLPAGGALLGLDPGTKSIGVAASDARRVVASPV
                     ATLARARFADTAEAVERLCRERDVAGLVVGLAVNMDGSEGPRAQSARALAANLEARLG
                     LPVLLWDERLSTSAVTRTLIEADLSRRRRRQVIDKLAATYILQGALDALARV"
     gene            14283..15053
                     /locus_tag="VGB88_03940"
     CDS             14283..15053
                     /locus_tag="VGB88_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646623.1"
                     /translation="MRKSLLICLLVLALGHVPLPAAGAGAGGDGYRAWANELVADWDG
                     QGSHERGTDVTGFGLAGLSLFMTHDEVIERLEAGGTAFDISETQRVTCVDDRIEDIRS
                     DAGKGANCVDYVIFTLQYGDGDAAHVKAEFKEDVYYEPGSSIVIKIEYTRDHAQRSVD
                     LDAAYDAVTSEYGYQSKYAHLKYYIGHFKKLKSYPYVDSEWQLPYLQMYLGSVSPISR
                     YSLVLSAGEALRKDLEARADQMVRDATEIREFRLPWGM"
     gene            15156..15425
                     /locus_tag="VGB88_03945"
     CDS             15156..15425
                     /locus_tag="VGB88_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9646624.1"
                     /translation="MTRDHAVARTEYRFTALFEPDEDGGYTVSFPALPGCLTHGATKD
                     EARRMAAEALEGYLETLQEDRMPIPVEHDVELPICEPVSVQLKAV"
     gene            15630..15917
                     /gene="gatC"
                     /locus_tag="VGB88_03950"
     CDS             15630..15917
                     /gene="gatC"
                     /locus_tag="VGB88_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9646625.1"
                     /translation="MALDRATVARVARLARIRIGEDELDRLAGELDNILNWIEQLNEV
                     DVAGVEPMTSVVAFELPERADRVTEGGRAEDIVANAPETAGSYFVVPKVVE"
     gene            15950..17404
                     /gene="gatA"
                     /locus_tag="VGB88_03955"
     CDS             15950..17404
                     /gene="gatA"
                     /locus_tag="VGB88_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX9646626.1"
                     /translation="MRTRLAAGETSAVELARAHLVAAEAARPLNAYITLAPERALAAA
                     AESDARRAHGAARPLDGVPIAVKDVYCTEGVLTTAGSHILDGFVPRYESTVTAKLAAA
                     GAIVVGKTNMDEFAMGSSNMTSYYGAVENPWRRAGDNRPLVPGGSSGGSAALVAARAV
                     PAALGTDTGGSIRQPAAFCGVVGLKPTYGRCSRWGIVAFASSLDQAGPFARTVRDAAI
                     VLGAIAGHDAKDSTSVPRPVPDYEAALTGDIRGLKVGVPKEYRIDGMAGEIEALWRQG
                     ADWLAGAGAEMVDISLPHTRYALPTYYIIAPAEASSNLARYDGVKYGLRVAGADLLDQ
                     YERTRAAGFGAEVRRRIMVGTYVLSAGYYDAYYLRAQRVRSLIARDFARAFEAVDVIL
                     APTAPFTAFAIGDKLDDPVAMYINDVFTVPASLAGLPAISVPAGLAGDGLPLGLQVIG
                     KPFDEETVLNAAHALEAEAGFTAVPAFTVDGAGA"
     gene            17401..>18139
                     /gene="gatB"
                     /locus_tag="VGB88_03960"
     CDS             17401..>18139
                     /gene="gatB"
                     /locus_tag="VGB88_03960"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013138.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX9646627.1"
                     /translation="MTDSTLMEGRTGPWEVIVGLEVHAQVVSESKLFSGAATAFGAEP
                     NSQVSPIDAAMPGMLPVINGRVVDQAIRTGLALGAEINRTSVFDRKNYFYPDLPPGYQ
                     ISQYSRPIVGRGAIAVDLADGGVRTVGITRLHLEQDAGKSLHDQHPSRTYVDLNRAGV
                     ALMEIVSEPDLRSAEEAALYLAKLRAILRTVGSCDGNMEQGSLRADANVSVRRPGEGL
                     GTRCEIKNLNSIRFLRRAIEVEARRQIG"
BASE COUNT         2637 a         6337 c         6606 g         2559 t
ORIGIN      
        1 tagcaggcgg tgctgaccac cccgcgccgg ccgccgccga cgatcaccgg cttgtcctgc
       61 aaggccgggt tgtcgcgctt ctcgaccgcg gcatagaacg cgtcgcagtc gagatgggcg
      121 atggcaagct ggttcagctc gccatggcgg accaggcgcg ggccgccgca ggccgggcag
      181 cgcagggctg cggcctgcgc cagccgggcg ccgcagtcgc ggcacaggcc ggggaggctc
      241 attgcccggc ctcggggtcg ggccacagcc acgccgcgcc gcgcacgccg tcgcgcgcga
      301 tcgccgccgc ctcgatcttg tggccgccga ggtccacccc gatgcgcatg gcgcgctcag
      361 tacccggccg cagccagctc ggactcgatc agcgcccgcg cctcgggcca gtggcggacc
      421 gcatgatggg cctcggacac cgggccgagc aggtccgcga gcctggtatc ggcgatcagg
      481 tggaggcggc gcacgcgcgg cgcgtgacgc gcgaccgagg cgtggtggcg cggcccgtcg
      541 tcgaggaaga acaccggtgc cgcgaccgcc tcggcgagcg cctcgaccac cggtcccttg
      601 gggccggtgt tggcgatcag cggatagtcc atgccgtggc gcgacagcgc ggcgcgccgg
      661 atcggcgcct gcgccgccgg gatgttgctc agcaccaata tctgggcgcg cgcgctgagc
      721 ccggcgagtg ccgccgccgc ccccggaacc ggctccagcg acgcggtgtg atgctcgaag
      781 aacgcgtcca gcaggccggc gaccgcggcg cgcggcagcg ccgtgtcgtc ggcgcggctg
      841 cggacgttgc cgtcaagcgc gaaggacatc cagtcgaagt agtggccgcg gcggtcgagg
      901 aagccctcga aggcggccat gaacacgaac agcacctcgt ccgcgtccga gacgatcagc
      961 gggcggtcgg gggcgaggtc gagcgcggcg atgcgcgcgt gcgcggccgc cgtgtcggcg
     1021 ggcgggccgg ccatctagtc tgacatcgcc gcgcccggca gcgccgcgcg ggcacgggcc
     1081 ggggtctccg gcgcgagccc ggcggcgtcg cagaaagcga gcagcagcgc ctcgtcgcca
     1141 agcacgaagt cgagcacgcc ggcaaggaag gcggcattgc cggcctcggc gcgcaacgtc
     1201 gcgaggtcgg cgccggacag cgccaggaac cgctcgcgcg cctccgtctc gccgaccacg
     1261 tgcgccaggg cctgcagggc gatggtctcg gccgcctcgg gtgtcatgag gcgattttag
     1321 aacaagcgcc ggcggcgtcc aggccccgcg gtggcggatg ctgggctatc taatcggagt
     1381 tccgatccag gcgaggaaga gaaaccagcc gactaagcgc ggttgccgca gtaggaccac
     1441 cgatcgccac aatcgcgacg actgcagcgg cttcccagcc atctagaatt gctatgaccc
     1501 cggccgccac gattcctagt aatcccagcg tcaaggcgtg atattgggcg cgattcattc
     1561 ggctttcgtc gccctcgaag gccctgtcct ccaggctatg gcgatgcctt ctctcgtcat
     1621 cccacccgcc taccatccgg tcggccaagc ccggaatgac tgcgtcgtac tgcttcagca
     1681 ttccaggcgg cgggatcggc cctcgatagg tccaactgtg agcggcgctg ataataagtt
     1741 gggcgtccgg gtcatcagga tcgatagccg cccgcctgag aatattcctt agcgccgcct
     1801 taagcctttg ggcgtcctcg tcctcggcag gaactggtaa cgcgtccttt gtcggcttct
     1861 tgtcgtcggt gtcaggcggc tcgggctgtt cgttcatccc gacgcaaccg atcgagcgaa
     1921 gcggtcatat catttccgac ggcctgccag tccgcccaga atgcgtcagc tgttgaccga
     1981 tgtaggatcg gtcggcgccg gattggcaga ggatcaaagc ccaaggaaaa tcccacccaa
     2041 tacgctcgcg ctggcgagtc taacgtgaac gcataatgtg gtagattttt caaccacttg
     2101 gccatcttga tcacgctcgt gaggcgatta tgttggtatc ccttcgtcaa tgtcaattgc
     2161 ccttgctacc ctcaccgatc cattcccaac cgttctcaac tgtaccttcc tcgcaacaag
     2221 gtcatcacca agaatgtcaa tctaggacaa ctttgttgcc ggattaggac accccgcccc
     2281 gatttagggc agtgcccggc ggtgcgacgg ttacaggttg tcgcgcggcg tcggcggcag
     2341 caggtggccg atgcggtcga gcaccgcggt cggctcgtcc cagaacgacg ctcccatgcc
     2401 ggcgcgcgac gaccagacca cggcggcggg catggtgccg aaccggtcga ccctgagcct
     2461 gatctcgtga ccgcggccga ggcgcgccgg ggcggcaagg aacgcgccac ccgcggacag
     2521 gttatggacc gagaacggga ccagctggtc gccgagatag aacgagccgg agagcagcac
     2581 caggcggcgc ggaaaccggc gcaggtcgcg attgctcgcg gtgtcgctca ggagccggtc
     2641 gatgatcgcc tggacctggc cgtggcggtc gatgaaggcg atgccgaact ggtcgccgtg
     2701 gttgcgcacg acctcggcgt agaggtcggg atggcgctcg atccggaacg cgaatgcgtt
     2761 cgccggctcg gcaccggacg ggattcggat cagggcgccg ctggcggaga tgttgagcac
     2821 ctggcaggcg atgtcgccgg cgcggttgcg gatcaccgcc ggcaggaaca ccgcggtgcg
     2881 ccgaaaccgc gggttgcggg gcgacgtgcg cggcgcgcgc cggtcgtgcc gcgggctcga
     2941 ccccgtccgc agctgtctcg cctggggaag ttccccgatc gctcccatcg cctgccccct
     3001 ccgcaactgg taaagctagg ggcgaattca cgcgcgccca gtgggcccga cgtttttccg
     3061 acatttcaag gagtggcggg ccgggcctca ccttgcccgc gagccggaaa ggttaacgtt
     3121 cgagcgccgc gcgcacgcaa tacgtgcgcg tatgccgcac gctttcaacc catgctacag
     3181 caacgcttgc cggccttccg gggccggcgg cgaagggggg acgccgcgct acaccgcggc
     3241 gcggcgctat ttgatcttgc cttccttgta cagcacgtgc ttgcgcgcga ccgggtcgta
     3301 cttcttgatc tcgagcttgc cggtcatggt gcgcgggttc ttcttggtga cgtagaagtg
     3361 cccggtgtcg gcggtgctgt tgaggcggat cttgagcgag ttcggcttgg ccatggtggt
     3421 gtccgtgggc gcggggcgcc gctcgaggtt gcgaggtcgg gcgcgacgat agctgccgcg
     3481 ccggcgctgt caagccccgg cggcggggcg cgccgtgcgc cggcgcccga tctcgaccga
     3541 cagggcgaag gcgccggtcg ccgcggccat cgccgccatc aggccgtgcg gggaccacag
     3601 ctccatcgcc accccgccga gcgccggtcc agcgacgctg ccgacgccgt acgcaagcgt
     3661 cagcccgaca ttgccggccg cgaggtcggc gccgcggaag cgctcgccaa gcagggtcat
     3721 ggcgaacatc cagaagctgc cgagcaggcc gccgatcgcg aacagcagca cgtggagcgc
     3781 gatcgggctc gcaccgaacg cggcgacggc gagcagcagc accgtccccg ccgcggcgct
     3841 gccggcaacc gccgcgcgcc ggtcggtgcg gtcggccagc cagccgagcg ggagctgaag
     3901 gacgatggcg ccgacggcca atgtggtcac cagccgggcg gcggcgggcg cctccagatc
     3961 gaggctgagc ccgtagaccg cgagcagcgc gaccatgccg ccgtcgagca ccccgaacag
     4021 ggccgcagcc gcggccgcca ccggcatcgc ccgcagcagc ctgagcgccc cgccggacgg
     4081 cacgccggcg atggttggcg cgctgcgcgc gccgatcagc accggcacga ctcccgacag
     4141 caccacgagc gcgccgacca ggaacggcag cgggccgtcg gcgccggtcg cggtgatgat
     4201 cagcggcccg agcgcgaagc cggcggcgag cgaggtggtg tagacagcga ccaggcgccc
     4261 gcgcacccgg tcgtcgatgg cggcgttgat ccaggtctcg ccggcgacga acagcaaccc
     4321 ctccgcgccg ccgaaggcga accggaccac cgcccaggcg gcgaacgagt cgaacgctgc
     4381 gagcaaggcg aggcacgcgg cgcagccgag gatgccggcg ttcatggcgc cgaccagccc
     4441 ggcgcgcctg atcacgccag gcgccagcgg cgcgcacagc atgatgccga ggaactgcgc
     4501 cgccgagttg acgccgatca gcgtctcgcc ggtgccgcgt gcctcgagca ccagggcgaa
     4561 caagggcagc cccagcccga tggtggtgcc gagcagcgtg gtcgcgctga tcgccgccac
     4621 caggccgcga accgggcccg ggcgcgcggc gggctcttgc accgtcatcg accgcgcttg
     4681 gcgcgtgtgc gcccgcgcgc gacctgggtc ttcgtgccgg cggcgtgttc gagctcgaag
     4741 cgcaaggtgc cggtgaccag gtcggcctcg gccaggcgga cctcgagcat ctcgccgagc
     4801 gcataggcgc ggccgctgtc gcgcccggtc aggcggtggc gccccgggtc gtgctccatg
     4861 tactcgccga agtcgcggat cgggatcagc ccgtcggcgc cggtgtcggc gagctggacg
     4921 aacagcccga accgggtcac cccggtgatg cgcgcggcga accgggcgcc cacccgctcg
     4981 gcgaggaagc gggtggcgaa gcggtcgagc gcatcgcgtt ccgccgtcgc cgcgcgtcgc
     5041 tcggtggccg agatctcggc gccgaccacg gcgaagtcct gatcggcgtc cttgggcagc
     5101 gcgcccgcgc caagaccgag cgcgccgatc aggctgcggt ggaccagcag gtcggcgtag
     5161 cgccggatcg gcgaggtgaa gtgggcatag cggcgcaggt tgagcccgaa atggccgatg
     5221 ttgtccgggc tgtactcggc ctgcgcctgg ctgcgcagga cggtcagcgc gacgctctcc
     5281 tcctcgggcg tgccgcgcgc cttgtcgagg atgtggttga actgcgccgt ggtcgcgtga
     5341 cgcccgcggg cgaggcgata gccgaggctc gacaggtagt tgcgcagcgc ttcgagcttg
     5401 tccagcggcg gctggtcgtg gatgcggaac atgcacggcg cgcggcgggc gaccagggtc
     5461 tcggcggcgg cgacgttggc caggatcatg aactcctcga tgatccggtg gctgtcgtag
     5521 cgcggccggg tctcgatggc ccggaccgcg ccggcgtcgt cgaacagcac ccgccgctcg
     5581 ggcaggtcga ggtcgagcgt gccgcgcttg cgccgcgccg cgagcaggcg gcggaaggcg
     5641 ccgtcgagcg gccggatcac gggctcggcg agcggctcga cctcggccgc tgcccggccg
     5701 tcgatcgccg cctgcactcc gcggtacgtg agccgcgccg ccgagcgcat cagcgcgcgc
     5761 tcgaagcggt ggcgcaccag cccgccgtcg ccgtcgatcc acaggtgtgc cgccacgcag
     5821 ccgcgatcct cgcccgggcg cagcgagcac agcccgttgg acagcgcctc gggcagcatc
     5881 ggcacgacca tgtcgggcag gtacaccgag ttgccgcgcc ggcgcgcctc gcggtcgacc
     5941 gcatcgcccg ggcgcacgta gtggccgacg tcggcgatcg ccaccaccag gtgccagccg
     6001 ccgcggctgt ccgggtccgg ctcggcgaac accgcgtcgt cgaagtcgcg cgcgtcctcg
     6061 tcgtcgatgg tgaccagcgg cagcttgcgc aagtcggcgc ggccgcgcac gccgagcggg
     6121 cgcgcggcct cggcccgggc caccgcctcg ggcgggaagt gggacgggat gccgtgctgg
     6181 ttgatcacca gcaggccgat cgagcgcggc gaatccatcc cctccagcac gtcgatcacc
     6241 cgtgcctggg gcaggccgag gcggtgcgcg ggcagggtct cggcgatcac caggtcgccc
     6301 gcgcgggcgt cgcgggcatc gccctgggcg acgaagaact cgctgcgcgc gcgccgctcg
     6361 accggcttga tgcggccgcc ggcgccgtcc ccggcgggcc gatagacgcc gaccagccgg
     6421 cccggccggc cgccgagcac ccggatcacg cgcccttcgt agaggtcctc ggcgatcttg
     6481 cgggtgcgca ccagcacccg gtcgccgacg cccggcgcgt gcgtgcgcgg gcgcagcgcc
     6541 ggcagcacgg tcaccgacgg cggcggcgcc tcgccgcgcc agtcgaccgg gcgggcaagc
     6601 gggtcgccgt cgaggtcgag gccggtgacc tcgaccacgc acacggcggg cagggcgccg
     6661 ccggcggcca gcgacttgcg ctgcccgcgc acgatcaggc cggcatccgc gagctcgcgc
     6721 agcacctcct tgagcgccgc gcggtccgcc cccttgagct tgaacgcgcg cgcgatctcg
     6781 cgcttgccga ccttgtggtc ggcggtttcg aggaattcga gtatctgctc gcgggacggc
     6841 agcccttcgg cgcggcgctg tttcggaccc gaagccttgg ccacggcgct cccgattcag
     6901 gcggggtcgg tgacggccgc ggcgcgcgcc ttgccggtgc tcttgcgtgc cgtcgaggcg
     6961 ggcttggcgc gcttgcgtcc cttggtcttc ttcgccgcct tctcggccag cagcggcagc
     7021 gcgtccgcga gcgtgacagc cgccgggtcg gcgtccttgg gcaggttggc gatcacgctg
     7081 ttgtgccgga cataggggcc gtagcggccg ctgtacagcg ctacctcgcg gtcgtcgtcg
     7141 ggatggcggc cgagcacccg cagccgggtc gagccgcggc gcgcgccgcc ggcctcggcg
     7201 agcaaggcga ccgcgcggtt gaggccgatg gtcagcacgt cgtcgccgtc cttgagcgac
     7261 ttgaagctgt cctggtgctt gatgtagggg ccgtaacggc cgacgccggc ggtgatcggc
     7321 gcgccggtct cgggatgcgt gccgacctcg cgcggcaggt cgagcaaggc aatcgccatc
     7381 gcgagatcga cggcctcggg gtccatgccc ttgggcagcg acacccgttt cggcttgtcg
     7441 ccgtcgccgg caccctcgcg ctggacatag aggccgtagg ggccgctgcg cagagtgacc
     7501 tcggcgccgt ccggcgcgct gccgagggcg cgcgggcctt ccgaaaggcc ggcgccttcg
     7561 ccgttgccgc cgttgaccac gaccagcggc cgggtgtagc ggcagtcggg gtaattggag
     7621 cagccgacga acgcgccgaa cttgccgagc ttgaggctca gccgcccgtc ggcgcaggcg
     7681 ggacacgcgc gcggatcgcg gcccgagccg ttcgccggga agatgtgcgg cccgaggaag
     7741 gaatcgagct tgtccaggat gtcgcgcacg cgcaggtggc cgatgtcgtc gaccgccagc
     7801 gagaacccgc tccagaactc gcgcagcacg tccttccagt cgagcttgcc gttcgagatc
     7861 aggtcgagcc ggtcctcgag ccgggcggtg aagtcgtact gcacgtagcg ctcgaagaag
     7921 ctctccagga acgcggtcac cagccggccg cggtcctcgg gcacgaaccg cttcttctcc
     7981 aggcggacgt agtcgcggtc ctggagcacc gtgatgatgt tggcgtaggt cgacggccgg
     8041 ccgatgccga gctcctccaa cctcttgacc agcgacgcct cgctgtagcg cggtggcggc
     8101 tcggtgaagt gctgcgcggg cgtgacctgg ccgaggctca gcgcctcgcc ctcggacacc
     8161 gccggcaggc ggcggccttc gccctcgtcg tcggcggcgg gctcgtcgcg gccctcggtg
     8221 tagagccggt aatagccgtc gaacgcgacc accgagccgg tcgcgcgcaa ggccaccttg
     8281 gcgtcggcgg aggcgatggt gaccccgacc tggtcgagca ccgcgggctc catctggctc
     8341 gccacggtgc gcttccagat cagctcgtac aggcgccctt cgtcctcgcc gatggcaagc
     8401 tcgcgcggat gccgggcggc gtcggtcggg cggatcgcct cgtgcgcctc ctgggcgttc
     8461 ttcgcccggt tggtgtagcg gcgcggcgac tcgggcaggt aggtgccgcc atagagccgc
     8521 tcgatcgcgg cgcgggccgc ggtcagcgcc tcgttcgaca aggtcaccga gtcggtgcgc
     8581 atgtagctga tcaggccgac cgtctcgccg gccaggtcga cgccttcgta caggcgctgg
     8641 gcgacgcgca tggtgcgctg ggcggtgaag ccgagcttgc gcgccgcctc ctgctgcaag
     8701 gtcgaggtcg tgaacggcgg cggcggggtg cggcggacct gcttgcgctc gaccgcggtc
     8761 accctgagag cgctcgccgc cactgcctgc gccgcgcgcg tcgccgcggc ctcgtcggcg
     8821 aggtcgaact tgtcgagctt ggcgccgtcg agggtgacca gccgcgcgct gaacctctcg
     8881 cccgatgcgc gcgcgagctc ggcatcgacg gtccagtact ggcgcgcctt gaacgcctcg
     8941 atctccatct cgcggtcgac gatcaggcgc agcgccaccg actggacgcg cccggccgag
     9001 cggctgcccg gcaccttgcg ccacagcacc ggcgacaggg tgaagccgac cagatagtcg
     9061 agcgcccggc gcgcctgctg ggcgttgacc aggtccatgt cgatgtcgcg cggatgcgcc
     9121 agcgcctcgc gcacagcatc gcgggtgacc tcgtagaaca ccacccgctc ggccgtcttg
     9181 ccctccaggg cgcccttggc gcgcaagtgg ttgagcacgt gccacgagat cgcctcgccc
     9241 tcgcggtcgg ggtcggtggc gagcagcagg cggcccgcgc ccttggcctc ggtggttatc
     9301 tgcttgagct gcttcgccga cttggggtcg gcgacatagt ccatggcgaa gtcctcgtcg
     9361 ggacgcaccg agccgtcctt cgacggcagg tcgcagacat ggccgtaggt ggcgacgacc
     9421 ctgtagccgg ggccgaggta cttgccgatc gtgcgcgcct tcgccggcga ctcgacgata
     9481 acgacgttgg tggtgtcggt ggtcttctcg ttcatcgctc gagataacgc ccttaattca
     9541 ttgatcagag atggatatcg agccccccgg atggcggatg acgcggccgg cgagctcgag
     9601 ctcgagcatc acgatcatga cctcggcggc agttaactgg cattggcgga ccagttcgtc
     9661 aaccaacagc ggcgtcggac cgagtgccgc catcacccgc tcccggtccg cgtccgtgac
     9721 gtccaactcg ctgatctcct tggacgaaat cccgatcccg gcgggcgcct ccggggtctc
     9781 gccgagccgt cttgaggtca tctcggcgag cgaggagagc acgtcctcgg ccgactcggt
     9841 gagcaccgcg ccctggcgaa tcagcaggtt ggtgccgccg tggcggggat cgagcggcga
     9901 gccgggaacc gcgaacacct cgcggccctg ctcgagcgca agacgcgccg tgatcaggct
     9961 gcccgagcgc cggctcgcct cgaccaccac cacgcccaga gcgaggcccg agacgattcg
    10021 gttgcggcgc gggaagtggc gcgcctgggg cgcgaggccg agcggctgct ccgagaccag
    10081 cgcgccggcg tcgcggatcg actcgtacag cgcctggttc tcggccgggt agacgatgtc
    10141 gatgccgccc gcctgggcgg cgacggtgcc gtgggcgagc gcgccggcat gggcctcggc
    10201 gtcgatgccg cgggcgaggc cggagaccac caccaggcca ccggcgccga gctcggcggc
    10261 gagcccgcgc gcgaaccgcc ggccgatgcc ggaggcattg cgcgcgccga ccacggcaac
    10321 cgaattgcgg tcgagcaggt cgagatggcc gagcgccgag atgatcggcg gcgggtcggg
    10381 gatcgccgcc agggcgcgcg gaaagccggg ctcgccccag gcgagcagat ggccgccgat
    10441 cgccgccagc gcgtccgcct cgcgcgccgc atcgtcgcgg ctgcaggcgc ggatgcgccg
    10501 gcgaaggccg ccgcggcggg cgagctcggg cagcatgtcg agcgccgccg ccgcgctgcc
    10561 ggtcgcgcgc aagagatcga agaacgtccg cgggccgacg ttctcgctgc gccacagccg
    10621 cagccaatcg agacgttcgc tcgcggcaag cccgcttcgc gccccatgca tcggcgcacc
    10681 atgggggtgg cggcgcaaac cggtcaagct ttcccgatgc gcggctcctt gccggacatc
    10741 aggcggcgga tgttggcgtg atggcggacc agcaccagca ccgcgatcac cgccatcagg
    10801 tcgacatgct ggggcagacc gggaagccag tagggcaaag tctcctggcg ctgcagcgcg
    10861 agcatggccc acagcatcgc cggtgccgcg atcatcgcga tcatcgaggc caccgagacg
    10921 atgcgcgtgg ccgcggcgac gatcgcgaat ccggcgccgg cggcgagcgc gaccggccag
    10981 ctgaccgcga acagcacccc gaacgcggtc gccacgccct tgccgccctg aaagcgcagc
    11041 cacaccggga acatgtggcc gagcaccacc gacaggccgg cgatgtactc gaagtcgggg
    11101 ccgtagaacc cggccgcggc gaccgccgcc gtgcccttgc cgaggtcgag cgccagcgtc
    11161 agcagggcga gagccgtgtg gccggtgcgc agcacgttgg tcgcgccgat gttgcccgaa
    11221 ccgatgcgcc ggatgtcgcc gagcccggcg acgcgggcga cgatcagccc gaacgggatc
    11281 gagccgatca ggtagcagac cgggatcgcg gcgtagtacg gccaggtgta ggcgaggccg
    11341 ccgagcaggt cgggcatggt cagggcgcgc cgtcgagcct gaacacggtc tcgccgccga
    11401 ccaccgtgcg cagcacccgg ccctgcaccg ggcgctcgtc atagggcgcg ttcttcgact
    11461 tgctccgcag cgccgaggtg tcgatccgcc acggccggtc gggatcgaac accaccaggt
    11521 cggcgggcgc gccgaccgcc aggcggccct gctcgaggcc gagcaggcgc gccggcgccg
    11581 cggtcatccg cgccagcacc tcgaccagcg acagcgcgcc ggcgtgcacc agctcgagcg
    11641 ccagcggcag catggtctcc agcccgatga tgccgaacgc cgcctgggcg aacgtggtgc
    11701 gcttcgagtc ctggtcgtgg ggcgcgtggt cgctcgcgat cgcgtcgatg gtgccgtcgg
    11761 cgagtccggc gaccacggcg gcgcggtcgg cctcgctgcg cagcggcggg cgcaccttgg
    11821 cgaaggtgcg gtagtcgtcg accgcggtct cgttcagcgc gaagtagggc ggcgcggtgg
    11881 cgcaggtcac cggctcgccg cgcgccttgg cccggcgcac gacatcgatc gccgccgcgg
    11941 tcgagacatg ggcgacgtga tagggcgcct cggcgcgcgc ggcgagccgc aggtcgcgct
    12001 cgatgatcac cgtctcggcc tccgcgggca tgccggcaag gcccagccgg ctcgccagct
    12061 cgccgtcgtt catcatgccg ccttcggtca gcgcccggtc ctccgggtgc tgcatcacca
    12121 gccggccgaa cggcttggcg taggtcagcg cgcgcagcat cacgcggctg tccatcaccg
    12181 cccggtcggc gtcgctgaag ccgaccgcgc cggcctcggc gagcatcccg atctcggtca
    12241 gctcccggcc ttcgaggccc ctggtgatgg ccgcgacggg atagatcttg actaggctgg
    12301 tctcgcgcgc gcgccgggcg atgaactcga tcagcgcgat ctggtcgatc ggcgggtcgg
    12361 tgttgggcat gcacgcgatc gaggtcaccc cgcccgcggc cgcggcgcgg ctggcggtgg
    12421 ccagcgtctc cttgtgctcg gcgccgggct cgcgcagatg cgcgcgcatg tcgaccagcc
    12481 cgggggcgag gcagtgcccg gcgcagtcga ccacgtcgat gccctccggc acgccgtcgg
    12541 cgaacagccc cgggccgagg tcggcgatgc ggcgcgcatc ggtcagcagg gcaccgcgca
    12601 ggtcgcgccc cgaggccgga tcgagcaggc gcgcattgac gtaggcgacg cgacccgggc
    12661 gcgcggccga cgcctgggtg ctcacgccac cgcctcggtc agcgccgggc cgcgccggcg
    12721 ggcgagcgca tcgaggcacg ccattcgcac tgcgacgccg agctccacct gatcgaggat
    12781 gacgctccgg gtcaggtcgt cggcgagctc gctgtcgatc tcgacgccgc ggttcatcgg
    12841 ccccgggtgc atgaccagcg cgtcgggcct ggccagcgcg agcttgtcgt ggtcgagccc
    12901 ccagaaatgg aagtactcgc ggatcgaggg cacgtagctg ccctgcatgc gctcggtctg
    12961 cagcctcagc atcatgacga tgtcgcagtc cttgaggccc gcgcgcatgt cgtggaacac
    13021 ctcgacgccg aagcgctcgg cggcggtcgg cagcagcgtc ggcggcgcga tcaggcgcac
    13081 ccgcgcgccc atgatgttga acaggtggat gttcgagcgc gccacccggc tgtggcggac
    13141 gtcgccgcag atcgccaccg tcagcccgtc gatgcggccg cgccggcggc ggatggtcag
    13201 ggcgtcgagc agcgcctggg tcgggtgctc gtggctgccg tcgccggcgt tgatcaccgc
    13261 gcactcgacc ttctccgaca gcaggttcac cgcgcccgag tcgttgtggc gcacgaccag
    13321 gatgtcgggg cgcatggcat tgagggtgag cgcggtgtcg atcagggtct cgcccttctt
    13381 gatcgagctc gcctgcacct gcatgttgat tacgtcggcg ccgaggcgct tgccggccag
    13441 ctcgaacgag gtccgggtgc gggtcgagct ctcgaagaac aggttgatca gggtctggcc
    13501 ctggagcagg gtctgcttct tgtcgacccg ccggttgagc gcgacgtagg actcggcgag
    13561 gtcgagcaga tagccgattt cctcggcggt catgtcggcg atgccgagca gatggtgccg
    13621 cgcgaacgga cggttggatg cggggccggt catcaaagga tcatcatacc gccgatgccc
    13681 gcagcggcaa gaccgcttcg cccggtcaga cgcgggcgag cgcgtcgagc gcgccttgga
    13741 ggatataggt cgcggcgagc ttgtcgatca cctggcgccg gcgccggcgc gacaggtcgg
    13801 cctcgatcag ggtacgggtg acggcactgg tcgacagccg ctcgtcccac agcagcacgg
    13861 gcaggccgag ccgggcctcg aggttggcgg cgagcgcgcg cgcggactgg gcgcgcggcc
    13921 cctcgctgcc gtccatgttg accgcgaggc cgaccaccaa cccggcgacg tcccgctcgc
    13981 ggcacaggcg ctccactgcc tccgcggtat cggcgaagcg cgcgcgcgcg agcgtcgcca
    14041 ccgggctcgc gaccacgcgc cgggcgtcgg aggcggcgac gccgatcgac ttggtgcccg
    14101 gatcgaggcc gagcagcgcg cccccggccg gcagcagggc ggcgaacgcg cgcaggtcgt
    14161 cggtggtcat ggcgcgctta tgaccggcac gcgcacgcgc cgcaagcgcg tccccgagcc
    14221 cggcgggcga ttcgccaccg aagtcgcagc ctgttagcat gctgctcgaa tgagactgcg
    14281 atatgcgcaa gtccctcttg atctgcttgt tggttcttgc cctgggtcat gtgccgctgc
    14341 cggcggcggg cgccggcgcg ggcggtgacg gctaccgggc gtgggcgaac gagctggtcg
    14401 ccgattggga cgggcagggc agccacgagc gcggcaccga cgtcaccggc ttcgggctcg
    14461 ccggcttgtc gctgttcatg acccatgacg aggtcatcga gcggctggag gccggcggca
    14521 ccgcgttcga catctcggaa acacagcgcg tgacctgcgt cgacgaccgc atcgaagaca
    14581 taaggagcga cgcgggcaaa ggcgcgaact gcgtcgatta cgtgatcttc accttgcaat
    14641 acggagacgg cgacgcggcg cacgtcaagg cagagttcaa ggaagacgtt tattatgagc
    14701 cgggctccag cattgtcatc aagattgagt acacccgaga ccacgcccaa agatcggtcg
    14761 acctcgatgc cgcctacgac gcggtgacga gcgagtacgg ctatcagtcc aaatatgccc
    14821 atctcaagta ttacattggg catttcaaga agttgaaatc ctacccttac gtcgactcgg
    14881 aatggcaact gccgtacctc cagatgtatc tgggcagcgt gagcccgatc agtcgctact
    14941 cgctggtgct gtcggcgggc gaggcgctgc gcaaggacct cgaggccagg gccgaccaga
    15001 tggtccgcga tgccaccgag atccgggagt tccggctgcc ctggggcatg tgaccgggca
    15061 ccggcgttcg cgtttccgcg ccctggcatg cgtcgtggca cccgatgcgg tcgcctgtta
    15121 tcttacggtc ccggaaagcg agaggtttgt gcaccatgac gcgtgatcat gcggttgcga
    15181 ggaccgagta ccggttcacg gcgctgttcg agccggacga ggacggcggc tacaccgtgt
    15241 cgttcccagc cctgcccggg tgcctgacgc atggcgcgac caaggacgag gcgcgccgga
    15301 tggcggccga ggcgctggaa gggtatctgg agaccctgca ggaagaccgc atgccgatcc
    15361 cggtcgaaca cgatgttgaa ttgccgattt gcgagccagt gagtgttcaa ctgaaggccg
    15421 tatgagccga cggctgccgc aggtcacgat tcatcaggga cgccgggctg acggtcgagc
    15481 aattcctcga tctcctttag cgtcgcagat ggcccggaaa ggattgccgg gcgcgccggc
    15541 gcggtggtag gtaggagggg cgccgcgagg cgcgcatccc acaacagcgg tccggcaggg
    15601 aggcgcccga ggcgtgacgg gaccaggcaa tggcgcttga tagggcgacg gtggcacgtg
    15661 tcgcgcggct tgcgcgcatc cgcatcggcg aggacgagct cgaccggctt gccggcgagc
    15721 tcgacaacat cctcaattgg atcgagcagc tcaacgaggt cgacgtggcc ggcgtcgagc
    15781 cgatgaccag cgtggtcgcg ttcgagctgc ccgagcgcgc ggaccgggtc accgagggcg
    15841 gccgcgccga ggacatcgtc gccaacgcgc ccgagaccgc cggctcctac ttcgtcgtgc
    15901 ccaaggtggt cgagtgaccg cggcatcggc cgggctgggt gtcgccgaga tgcggacacg
    15961 cctggccgcg ggcgagacca gcgcggtcga gcttgcgcgc gcgcacctcg tggcggccga
    16021 ggcggcgcgg ccgttgaacg cctacatcac cttggcgccc gagcgggcgc tcgcggcggc
    16081 ggcggagtcg gacgcgcggc gggcgcacgg cgcggcgcgc ccgctcgacg gcgtgccgat
    16141 cgcggtcaag gacgtgtact gcaccgaggg cgtgctgacc acggcgggct cgcacatcct
    16201 cgacggcttc gtgccgcgct acgagtcgac ggtcaccgcc aagctcgccg ccgccggcgc
    16261 gatcgtcgtc ggcaagacca acatggacga gttcgcgatg ggctcgtcga acatgaccag
    16321 ctattacggc gcggtcgaga acccgtggcg gcgcgccggc gacaaccggc cgctggtgcc
    16381 gggcggaagc tcgggcggct cggcggcgct ggtcgcggcg cgagcggtgc cggcggcgct
    16441 cggcaccgac accggcggct cgatccgcca gccggcggcg ttctgcggcg tggtcgggct
    16501 caagccgacc tacggccgct gctcgcgctg gggcatcgtc gcgttcgcaa gctcgctcga
    16561 ccaggccggg ccgttcgcgc gcactgtccg cgacgccgcg atcgtgctcg gcgcgatcgc
    16621 cggccacgat gccaaggact cgacctcggt gccgcgcccg gtccccgact acgaggcggc
    16681 gctgaccggc gacatccgcg gcctcaaggt gggcgtgccg aaggagtacc ggatcgacgg
    16741 catggccggg gagatcgagg cgctgtggcg ccagggcgcc gattggctcg cgggcgccgg
    16801 cgccgagatg gtcgacatct cgctgccgca cacgcgctac gcgctgccca cctattacat
    16861 catcgcgccc gccgaggcgt cgtcgaacct cgcccgctac gacggcgtca agtacgggct
    16921 gcgcgtcgcc ggcgccgacc tcctggacca gtacgagcgc acccgcgccg ccggcttcgg
    16981 cgccgaggtc cgccggcgga tcatggtcgg cacctacgtg ctgtcggccg gatactacga
    17041 cgcctattac ttgcgcgcgc aacgggtgcg cagcctgatc gcgcgcgact tcgcccgcgc
    17101 gttcgaggcc gtcgacgtga tcctggcgcc gaccgcgccg ttcaccgcgt tcgcgatcgg
    17161 cgacaagctc gacgacccgg tcgcgatgta catcaacgac gtgttcacgg tgcccgccag
    17221 cctcgccggg ctgccggcga tctcggtgcc ggcggggctc gcgggcgacg gcctgccgct
    17281 cggcctgcag gtcatcggca agccgttcga cgaggagacg gtgctgaacg cggcccacgc
    17341 gctggaggct gaggccggat tcaccgccgt gcccgcgttc accgtcgatg gagccggcgc
    17401 atgaccgaca gcacgctgat ggagggccgg accggcccgt gggaggtgat cgtcgggctc
    17461 gaggtccacg cccaggtggt ctccgagtcc aagctgttct cgggcgcggc gaccgcgttc
    17521 ggcgccgagc ccaactccca ggtctcgccg atcgacgccg ccatgcccgg catgctgccg
    17581 gtgatcaacg gccgcgtcgt cgaccaggcg atccgcaccg ggctcgcgct cggcgccgag
    17641 atcaaccgga cctcggtgtt cgaccgcaag aactacttct atcccgacct gccgcccggc
    17701 tatcagatct cgcaatactc gcgcccgatc gtcggccgcg gcgcgatcgc cgtcgacctc
    17761 gcggacggtg gcgtgcgcac cgtcggcatc acccggttgc atctcgagca ggacgcgggc
    17821 aagagcctgc acgaccagca tccgagccgc acctatgtcg acctcaaccg cgccggcgtc
    17881 gcgctgatgg agatcgtctc cgagcccgac ctcagaagcg ccgaggaggc ggcgctgtat
    17941 ctcgccaagc ttcgcgccat cctgcgcacc gtcggcagct gcgacggcaa catggagcag
    18001 ggctcgctgc gcgccgacgc caacgtctcg gtgcgccggc cgggagaggg gctcggcacc
    18061 cgctgcgaga tcaagaacct caactcgatc cgcttcctca ggcgcgcgat cgaggtcgag
    18121 gcccggcgcc agatcggga
//
LOCUS       DASWHU010000113         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2632356, whole genome shotgun sequence.
ACCESSION   DASWHU010000113 DASWHU010000000
VERSION     DASWHU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632356"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1844)
                     /gene="mutL"
                     /locus_tag="VGB88_03965"
     CDS             complement(3..1844)
                     /gene="mutL"
                     /locus_tag="VGB88_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX9646628.1"
                     /translation="MSLRLLPETLVNRIAAGEVIERPAAALKELLENALDAGARNIEA
                     ELRDGGRSYLSVSDDGCGMTPEDLSLAVERHATSKLPEDDLVHIATLGFRGEALPSIA
                     AVSRMTVTSRAAGAGEAWRLAVEGGRKGAVEPAALGRGTRVEVRDLFYATPARLKFLK
                     SARAEQMQARDALTRLAMAHHGVGFVLGDGQRDLLNLPAAQGELFESRLRRLAAILGR
                     EFADNALPIDAEREGVRLTGHIGLPTLNRASAQAQYLFVNGRPVRDKLLYGAVRGAYH
                     DVLAHDRHPMLALFLDLSPDLVDVNVHPAKTEVRFRDPALVRGLIVSAAKHALAEAGH
                     RASTTVSTAALGAFAPGAMRPGRGSGHVYGTGGGATLPRGLAEAAAAYHAPLPGLAQA
                     PAARTAHAAPRDGMGGPEANGADEALAEAYPLGAARAQLHGTYIVAQNSDGIVIVDQH
                     AAHERLVYERMKEQLAAGRVASQLLLLPEVVELDEPAAERLAARAEELAEIGLRLEAF
                     GPGAVVVRETPALLGEVDIQGLVRDLADELGELGEALALKSGLEAVCSTMACHGSVRA
                     GRLLNAAEMNALLRQMEATPHSGQCNHGRPTYVELKLADIERLFGRR"
BASE COUNT          260 a          739 c          734 g          273 t
ORIGIN      
        1 gctcaccgcc ggccgaacag gcgctcgatg tcggccagct tcagctcgac ataggtcggc
       61 cggccgtggt tgcactggcc ggagtggggc gtcgcctcca tctggcgcag gagcgcgttc
      121 atctccgccg cgttcagcag ccggccggcg cgcaccgagc cgtggcaggc catggtggag
      181 cagaccgcct cgaggccgga cttgagcgcc agcgcctcgc ccagctcgcc cagctcgtcg
      241 gcgagatcgc gcaccaggcc ctgaatgtcg acctcgccca acagggccgg ggtctcgcgc
      301 accaccaccg cgccggggcc gaaggcctcg agccggaggc cgatctccgc cagctcctcg
      361 gcccgcgcgg cgagccgctc cgcggcgggc tcgtcgagct ccaccacctc cggcaacagc
      421 agcagctgcg aggcgacccg gccggccgcg agctgctctt tcatgcgctc gtagaccagg
      481 cgctcgtggg ccgcatgctg gtcgacgatg acgatgccgt cgctgttctg cgccacgatg
      541 taggtgccgt gcagctgggc gcgcgctgcg cccaggggat aggcctcggc cagcgcctcg
      601 tcggcgccgt tggcctccgg tccgcccatt ccgtcgcgcg gcgcggcatg cgccgtcctc
      661 gcggccgggg cttgggcgag gcccggcagg ggcgcgtggt aggccgccgc cgcctcggcg
      721 aggccgcgcg gcagggtcgc accgccgccg gtgccataga cgtgcccgct gcccctgcca
      781 ggcctcattg cgccgggcgc gaaggccccc agcgccgcgg tcgagaccgt ggtcgaggcg
      841 cggtgccccg cctcggcgag cgcgtgcttg gcggcgctga cgatcaggcc gcgcaccagg
      901 gcgggatcgc gaaagcgcac ctcggtcttc gccgggtgca cgttgacgtc gaccaggtcc
      961 ggcgacaggt cgaggaacag ggccagcatg gggtggcggt cgtgcgccag gacgtcatga
     1021 taggcccccc tgaccgcgcc atagagcagc ttgtcgcgca ccggccggcc gttgacgaag
     1081 agatactgcg cctgggcgct ggcgcggttg agggtcggca ggccgatatg gccggtgagg
     1141 cgcacgccct cgcgctcggc gtcgatcggc agcgcgttgt cggcaaactc gcggccgagg
     1201 atcgccgcca ggcgcctcag gcgggactcg aacagctcgc cctgggcggc cggcaggttg
     1261 aggagatccc gctggccgtc gccgagcacg aaaccgaccc cgtggtgcgc catggcgagc
     1321 cgggtgagcg cgtcgcgcgc ctgcatctgc tcggctcgcg cggacttgag gaacttgagc
     1381 cgcgccgggg tcgcgtagaa cagatcgcgc acctcgaccc gggtgccgcg gccgagcgcc
     1441 gccggctcga ccgcaccctt gcgcccgccc tcgacggcaa ggcgccacgc ctccccggcg
     1501 ccggcggcgc ggctggtgac ggtcatgcgg ctgaccgccg cgatcgacgg cagcgcctcg
     1561 ccgcggaagc cgagggtcgc gatgtggacc agatcgtcct cgggcagctt cgaggtggcg
     1621 tggcgctcga cggcgagcga caggtcctcc ggcgtcatgc cgcagccgtc gtcgctcacc
     1681 gagagatagc tccgcccgcc gtcgcgcagc tccgcctcga tgttccgcgc gccggcatcg
     1741 agggcgttct ccagcaattc cttgagggcg gccgccggcc gctcgatcac ctcgccggcg
     1801 gcgatgcggt tgaccagggt ttccggcagc aggcgcaggc tcatggcaac gactcgtcgg
     1861 ctcgcgccgc cgtctgcaga tagtcccgcg ccaggcgctt gccctcgtcc acggccggct
     1921 ggtcgaaggg gttcacgccg agcagatcgg cggcaatcac cgtttctagc atgaagtgca
     1981 tcatcagggc acccagctgg tactcg
//
LOCUS       DASWHU010000114         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1155612, whole genome shotgun sequence.
ACCESSION   DASWHU010000114 DASWHU010000000
VERSION     DASWHU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155612"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGB88_03970"
     CDS             <1..193
                     /locus_tag="VGB88_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEX9646629.1"
                     /translation="LDHVCATMACHGSVRAGRRLTVAEMNALLREMEQTPHAGQCSHG
                     RPTYVELRLADIERLFGRR"
     gene            complement(324..1148)
                     /locus_tag="VGB88_03975"
     CDS             complement(324..1148)
                     /locus_tag="VGB88_03975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9646630.1"
                     /translation="MRRLFRVAAMVAAATVWAQAAVAAALELHGRPIQGGLMRGHVAP
                     GCVVTLDSAEVRVADDGEFVIGFGHDHGEWSIVKALCEHGPSARRFLRIEQRSYPEQR
                     IDGLPEAMVTFDEETLARIRTDQARVDAARAADSDLAGHRAALLWPATGPITGTFGAR
                     RVLNGEPRQPHFGIDIEAAPGTSVRAAAAGRVTLAEELYLSGKTVILDHGHGLGTSYL
                     HLGEIAVAVGEEVAQGDVLGTVGMSGRATGAHLDWRITWYRARLDPALLAGPMPGQ"
     gene            complement(1145..2620)
                     /gene="xseA"
                     /locus_tag="VGB88_03980"
     CDS             complement(1145..2620)
                     /gene="xseA"
                     /locus_tag="VGB88_03980"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9646631.1"
                     /translation="MTDAPDLAGNVPEYTVSELSSALKRTVEGAFARVRVRGEISGYH
                     AHGSGHLYFALKDESAVLDAVVWRTTAARLRVAPEDGLEVVCEGRLSTYAQRSKYQLV
                     VDSIQVAGAGALLALVEERRRKLAAEGLFDAARKQPLPFLPEVIGVVTSPTGAVIRDI
                     LHRLADRFPRTVLVWPVRVQGEGAAEQVAAAIRGFNALPEGGAVPRPELLIVARGGGS
                     IEDLWAFNEEVVVRAAAESRIPLISAVGHETDTTLIDFAADVRAPTPSAAAEMAVPVR
                     AELAVTLRAHDHRLAAAAARLLRELGDRIEGLGRGLPRPEALLGEAGQRLDERAATLA
                     RVMGALIERRHAKLVGAAAGLRSPGERLAEARGRLAVAHRALAAAARTRLREARIGLD
                     RALGEARIARALARLAGDRHKRLERAAALLETLGYRNVLARGFAVVYGADGALVPRAE
                     AARRAHRLEIEFHDGRVGAQVLAPRSPAKRGAGDGGQGSLL"
     gene            2720..>3996
                     /gene="purD"
                     /locus_tag="VGB88_03985"
     CDS             2720..>3996
                     /gene="purD"
                     /locus_tag="VGB88_03985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9646632.1"
                     /translation="MKVLVIGGGGREHALCWAIAASPLCDALFCAPGNGGIAEEARCV
                     AIDPLAFDDIVAFCRKQKIEFVVVGPEAPLVAGLVDRLEAEGIAAFGPTRKAARLEGS
                     KAFAKTFCLDNRIPTAGFETFTDADRAKDYILAKNRPMVVKADGLAAGKGVILGLTVQ
                     ASARAVDVIFGPQFSVAGQRVVIEEFLEGEEASFFALVDGAHALPLASAQDHKAVGEG
                     DTGANTGGMGATSPAPVIDAAMAARVMGEIVTPTVRAMAAAGTPYKGVLYAGLMITDD
                     GPKLLEYNVRFGDPECQVLMMRLRSDLLPALISARDGELEHFDLRWRDEAALCVVMAA
                     NGYPGAYAKGGEIRGLDALAADERQGRLKVFHAGTKRADGRLVAAGGRVLGVTALGKT
                     VKEAQARAYGAVDRIDWPGGFCRRDIGWRAVER"
BASE COUNT          511 a         1480 c         1499 g          506 t
ORIGIN      
        1 tctcgaccac gtctgtgcga ccatggcctg ccacggcagc gtgcgcgccg gtcgccggct
       61 gacggtcgcc gagatgaacg cgctcctgcg cgagatggaa cagacccccc acgccggcca
      121 gtgcagccac ggccgcccga cttatgtcga gctccgcctc gccgacatcg agcgcctctt
      181 cggccggcgg tagacgaccg gccgccccag cggccctttt cgtcatgccg gccggcgagc
      241 cggcatccag tagacggcgc gtgccgaccc acggaccccg gatacgccct gcgggcgttc
      301 cggggtgacg ccgaggtccg cgcctactgc cccggcatcg ggccggcgag gagggcgggg
      361 tcgaggcggg cgcggtacca ggtgatgcgc cagtcgagat gggcgccggt ggcgcgcccg
      421 ctcatcccca ccgtgccgag cacgtcgccc tgcgcgactt cctcgccgac cgcgaccgcg
      481 atctcgccga gatggaggta gctggtgccg agcccgtggc cgtggtcgag gatcaccgtc
      541 ttgccgctca gataaagctc ctcggcgagc gttacccggc cggccgcggc ggcgcgcacc
      601 gacgtgcccg gggcggcctc gatgtcgatg ccgaaatggg gctggcgcgg ctcgccgttg
      661 agcacccggc gggcgccgaa ggtgccggtg atcgggccgg tcgccggcca cagcagcgcc
      721 gcgcggtggc cggcaaggtc gctgtcggcg gcgcgcgcgg cgtcgacgcg ggcctggtcg
      781 gtgcggattc gcgccagcgt ctcctcatcg aaggtgacca tcgcctcggg cagaccgtcg
      841 atgcgctgct cggggtagct ccgctgctcg atgcgcagga accggcgcgc cgaggggccg
      901 tgctcgcaca gcgccttgac gatcgaccat tcgccgtggt cgtggccgaa gccgatgacg
      961 aactcgccgt cgtcggcgac gcgcacctcg gcactgtcga gggtgaccac gcagcccggc
     1021 gcgacgtggc cgcgcatcag cccgccctgg atcgggcggc cgtgcagctc gagcgcggcg
     1081 gccaccgccg cctgcgccca gaccgttgcc gcggcaacca tcgccgcgac acggaacagg
     1141 cgcctcacag caggctgccc tggccgccgt cgccggcgcc gcgcttggcg gggctgcgcg
     1201 gcgcgagcac ctgggcgccg acgcggccgt cgtggaactc gatctccagc cggtgcgcgc
     1261 gccgcgccgc ctcggcgcgc ggcaccagcg cgccgtcggc gccgtagacc acggcgaagc
     1321 cgcgcgcgag cacgttgcgg tagccgaggg tctcgagcag cgccgcggcg cgctcgaggc
     1381 gcttgtgccg gtcgcccgcg agccgcgcga gagcgcgcgc gatgcgggcc tcgccgagcg
     1441 cccggtcgag gccgattcgc gcctcgcgca agcgcgtgcg cgccgccgcg gccagcgcac
     1501 ggtgcgccac cgcgagccgg ccgcgcgcct cggcgagccg ctcgccgggg ctcctgaggc
     1561 cggcggcggc gcctaccagc ttggcgtggc ggcgctcgat gagcgcgccc atcacccgcg
     1621 ccaaggtcgc ggcgcgctca tccagccgct ggccggcctc gccgagcagc gcctcgggcc
     1681 gcggcaggcc gcgccccagg ccctcgatcc ggtcgccgag ctcgcgcagc agccgcgccg
     1741 ccgccgccgc cagccggtgg tcgtgcgcgc gcagggtgac cgcgagctcg gcgcgcaccg
     1801 gcaccgccat ctcggccgcc gcgctcggcg tcggcgcgcg cacgtcggcg gcgaagtcga
     1861 tcagggtggt gtcggtctcg tggccgaccg cgctgatcag cgggatgcgg ctttcggccg
     1921 cggcgcgcac caccacctcc tcgttgaacg cccacaggtc ctcgatgctg ccgccgccgc
     1981 gggcgacgat cagcagctcc gggcgcggca ccgcgccgcc ctcgggcagc gcattgaagc
     2041 cgcggatcgc ggcggcgacc tgctcggccg cgccctcgcc ctgcacccgc accggccaca
     2101 ccagcacggt gcgcgggaag cggtcggcga gccggtgcag gatgtcgcgg atcaccgccc
     2161 cggtcggcga ggtgaccacg ccgatcacct cgggcaggaa cggcagcggc tgcttccgcg
     2221 ccgcgtcgaa caggccctcg gcggcgagct tgcgccggcg ctcctccacg agcgcgagca
     2281 gcgcgccggc gccggcgacc tggatcgagt cgaccaccag ctggtacttc gagcgctggg
     2341 cataggtcga cagccggccc tcgcacacca cctcgagccc gtcctcaggc gccaccctga
     2401 gccgcgcggc ggtcgtgcgc cagaccaccg cgtcgagcac cgcggactcg tccttgagcg
     2461 cgaagtacag gtggcccgag ccgtgggcgt gatagccaga gatctcgccg cgcacgcgca
     2521 cccgcgcgaa cgcgccctcg accgtgcgct tgagcgcgct cgaaagctcg ctcaccgtgt
     2581 attcggggac gttgccggcg aggtcggggg cgtcggtcat ggcggcaggg cgatcatagc
     2641 cgaggccccg ccggagcgtc cattgacagg gccggcgcca tcgggtgcag tcctgcccga
     2701 tggcttcggg tgcgggcgca tgaaggttct ggtgatcggc ggcggcgggc gcgagcacgc
     2761 gctgtgctgg gcgatcgccg cctcgccgct gtgcgacgcg ctgttctgcg cgcccggcaa
     2821 cggcggcatc gccgaggagg cgaggtgcgt cgcgatcgac ccgctcgcct tcgacgatat
     2881 cgtcgccttc tgccgcaagc agaagatcga gttcgtcgtc gtcgggcccg aggcgccgct
     2941 ggtcgcggga ctcgtcgacc ggctcgaggc ggagggcatc gccgcgttcg ggccgaccag
     3001 gaaggcggcg cggctcgagg gctcgaaggc gttcgccaag acgttctgcc tcgacaaccg
     3061 catcccgacc gcgggtttcg agacctttac cgacgccgat cgggccaagg actacatcct
     3121 ggcgaagaac cggcccatgg tggtcaaggc cgacgggctc gccgccggca aaggggtgat
     3181 cctcgggctc accgtccagg cttcggcccg ggccgtcgac gtcatcttcg gcccgcagtt
     3241 ctccgtcgcc ggccagcggg tggtgatcga ggagtttctc gaaggcgagg aggcgagctt
     3301 tttcgccctg gtcgacggcg cgcacgcgct gccgctcgcc tccgcccagg accacaaggc
     3361 ggtgggcgag ggcgataccg gcgccaacac cggcggcatg ggcgccacct cgccggcgcc
     3421 ggtgatcgat gcggcgatgg cggcgcgggt gatgggcgag atcgtcaccc ccaccgtgcg
     3481 cgcgatggcc gctgccggca cgccgtataa gggcgtgctt tatgccgggc tgatgatcac
     3541 cgacgacggg ccgaagctgc tggaatacaa cgtccgcttc ggcgaccccg agtgccaggt
     3601 gctgatgatg cggctgcgct ccgacctgct gccggcgctg atctcggcgc gcgacggcga
     3661 gctcgagcat ttcgacctgc gctggcggga cgaggcggcg ctgtgcgtgg tgatggcggc
     3721 gaacggctat ccgggcgcct acgccaaggg cggcgagatc cggggactcg atgcgctcgc
     3781 cgcggacgag cgccaaggca ggctcaaggt gttccacgcc ggcaccaagc gcgcggacgg
     3841 gcgcctggtc gccgccggcg ggcgcgtgct cggcgtgacc gcgctcggca aaacggttaa
     3901 ggaagcgcag gcgcgcgcct acggggccgt cgaccggatc gactggccgg gcggcttctg
     3961 ccggcgcgac atcggctggc gggcggtcga gcgcga
//
LOCUS       DASWHU010000115        16188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1524517, whole genome shotgun sequence.
ACCESSION   DASWHU010000115 DASWHU010000000
VERSION     DASWHU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524517"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..2038)
                     /gene="mrdA"
                     /locus_tag="VGB88_03990"
     CDS             complement(158..2038)
                     /gene="mrdA"
                     /locus_tag="VGB88_03990"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9646633.1"
                     /translation="MNQQQRARQRLFARRTVLLAAGKLALLSVLGGRLYYLQVVQGHQ
                     YRLLAEDNRINIKLLSPPRGRIFDRFGAELAVNRQNYRLVLVPEQAGDTDATLAAVGG
                     YVPLGEDELERVRAEIARKRAFVPVVLREHLSWRQVAAIEINAPYLPGISIEVGQLRQ
                     YPHAAALAHVLGYVAAVSESELTGDPLLELPDFRIGKNGVEKIHDLSLRGRAGTQQVE
                     VNAHGRVIRELSRQEGRPGDDVKLTLDLGLQEFAAARLGAESAAAAVLDIATGEVLAL
                     TSTPGFDPNLFNQGLTRKQWNELVENPRAPLINKAIAGQYPPGSTFKVVAALTALDSG
                     LLGPGHSVYCPGHLQLGNRRFHCWKKGGHGRLAMVEAISRSCDVYFYDVALKVGVDRL
                     AEMAHRLGLGARLGLDLPGEAAGLMPTQAWKRATYGEPWHKGETLNTGIGQGYVLTTP
                     LQLAVACARIAGGRAVVPRLALDVRHDGAEAPAAAPAAALLGIDPAALEIVRAGMNKV
                     VNDRTGTAWRARIEDEALAMAGKTGTSQVRRISKREHETGVVKNEDKPWAERDHALFI
                     AFAPVAAPRYACAVVVEHGGGGSAVAAPMVRDILIEAQGRDSARTAPPVATRPPADPG
                     RA"
     gene            complement(2035..2571)
                     /gene="mreD"
                     /locus_tag="VGB88_03995"
     CDS             complement(2035..2571)
                     /gene="mreD"
                     /locus_tag="VGB88_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX9646634.1"
                     /translation="MTEENELWHRIESQLRAMLPFVLTLFLAVLCAVPLGVPGLAPVT
                     PLLPAIAVFYWTVYRPDLLPMTATLLVGLVHDALTGAPFGLTALVLLVLQAVAGTQRR
                     FFTAKALPAAWLGFAVIGTGAVALWWLAASALALRPMPISPSLFQAILTVALYPVIAW
                     PLGTVQRELLGDLEARAR"
     gene            complement(2568..3512)
                     /gene="mreC"
                     /locus_tag="VGB88_04000"
     CDS             complement(2568..3512)
                     /gene="mreC"
                     /locus_tag="VGB88_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9646635.1"
                     /translation="MQRRPPGSISRVAVPVRALLHRFGLLLVIGLSVTLMVVGKAEPE
                     AMGRVRAAVVDVFAPVLDGLSRPVETVNQMIAEVRGLLTLRTENAELTEAVARLRRWE
                     QAARRLEHENAALRALLGLRLEAKPAFVSARVIGDSASAFVRTVLVNVGAGDGIAAGQ
                     AALTGEGLAGRVVEVGRRASRVLLVTDLNSRVPVVIEGSGERAIAAGDNSDTLRLEYL
                     PLEYAPALGARVVTSGHGGVLPPGLPVGVVVAVGEAGVRVEPAADLARLEFVRLVAWD
                     GPRFEPLPEAGEDGEEPVAGAPGAPEMASGQGGGASAQ"
     gene            complement(3601..4641)
                     /locus_tag="VGB88_04005"
     CDS             complement(3601..4641)
                     /locus_tag="VGB88_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9646636.1"
                     /translation="MFSRLMGLMSNDMAIDLGTANTLVYVAGRGIVLNEPSVVALIDQ
                     RGRKQVLAVGHEAKEMLGRTPGNIEAIRPLRDGVIADFEVAEEMIKHFIRKVHNRRSF
                     ASPLIVVCVPSGSTAVEQRAIQESAESAGARRVYLIEEPMAAAIGAGLPVTEATGSMV
                     VDVGGGTTEVAVLSLGGIVYAKSVRVGGDKMDEAIIGYIRRNHNLLIGESSAERIKMT
                     IGGAMPPEDGNGVTMEIKGRDLMNGVPKEIVISERQIAESLAEPVGAIIETVKVALEN
                     TPPELAADIVDKGIMLTGGGGLLRNLDHVLRQATGLPVSIAEEALTCVALGTGRALEE
                     MRTLKHVLHSAY"
     gene            complement(4749..6308)
                     /locus_tag="VGB88_04010"
     CDS             complement(4749..6308)
                     /locus_tag="VGB88_04010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9646637.1"
                     /translation="MTRTDDRERVIIFDTTMRDGEQSPGASMTLEEKLQIAKVLEEMG
                     VDVIEAGFAIASKGDFEAINEISKVVTGSVVCSLARAGRKDIDRAAEALVPAKRKRIH
                     TFISTSPLHMKHKLQMTPEDVHQAVIDSVTRARNLCDDVEWSPEDGTRTEHDFLCRTV
                     ESAIKAGAGTINVPDTVGYTTPEEFTALIRMLFERVPNIDRAVLSVHCHNDLGLAVAN
                     SLAAIRAGARQVECTVNGLGERAGNAAMEEIVMAIRTRNDLLPFHTNIRTEHIMRASR
                     LVSAVTGFTVQPNKAIVGANAFAHESGIHQDGMLKHAGTYEIMTPESVGLTKSTLVMG
                     KHSGRHAFKTKLSELGYEVGDNALNDAFARFKDLADKKKDIYDEDIVALVDDAVSHQH
                     ERIKLVSLAVHCGTGMRPRAALTLEVDGVEGATEAEGDGPVDAVFRAIGELFPHHAKL
                     QLFQINAVTEGTDAQAEVSVRLEENGRSVNGQSADTDTMVASARAYVNALNKLLVKRE
                     KSAPAALVARA"
     gene            complement(6656..7675)
                     /gene="ilvC"
                     /locus_tag="VGB88_04015"
     CDS             complement(6656..7675)
                     /gene="ilvC"
                     /locus_tag="VGB88_04015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9646638.1"
                     /translation="MRVYYDRDADLNLIKAKNVVVIGYGSQGHAHAANMRDSGVPQVA
                     VALREGSASAKKAKDAGFKVMGVTEAAKWADVMMMATPDELQAGIYRDHLRGNMKDGA
                     ALAFAHGLNIHFGLIEPRADLDIFMIAPKGPGHTVRSEYVRGGGVPCLVAVDRDASGN
                     ALEIALSYASAIGGGRAGVIETSFREECETDLFGEQVVLCGGLTSLIAAGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMRYSISNTAEYGDYTRGPRLIDDHVRAEMK
                     RVLDDIQSGRFTREWMLECQVNQPSFKALRRRAAQHPIEEVGAKLRAMMPWIAEHRLV
                     DKTKN"
     gene            complement(7725..8234)
                     /gene="ilvN"
                     /locus_tag="VGB88_04020"
     CDS             complement(7725..8234)
                     /gene="ilvN"
                     /locus_tag="VGB88_04020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9646639.1"
                     /translation="MSEIIETHTIACIVDNEFGVLARVVGLFSGRGYNIESLTVAEVD
                     PERNLSRITLVTSGTPMVIEQIKAQLGRLVPVHAVIDLTEGGPHVSRELALIKVCGTG
                     DKRVESLRIADVFRARVVDSTIESFVFELTGTTDKIEAFIALMRPLGLTEVARTGAAA
                     IARGAGGPQ"
     gene            8374..9831
                     /locus_tag="VGB88_04025"
     CDS             8374..9831
                     /locus_tag="VGB88_04025"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9646640.1"
                     /translation="MPRALRFLICALLLAAWPAAAQQAPSAAEVGRAEARNRTTLEAF
                     QAGDAEAAVAAARGAAEANLAALGPGHEQTAHAWFNLATILSRQGDYDQAEALHRRAL
                     AAREAALGADHVDVADSLLALGQLNRNRGHYADAEQQFARALAIRTAALGDGHPEVAQ
                     VHGELARLYRVQDRFAEAEANQAAALAIFERELGPDHPLVADALSELALIHHNAGAYD
                     QAEPSYLRVLKMRETRFGPEHVLVARSLANLGALYRSKGDLARAEDYYRRALAAKRAA
                     LGPAHPDVAVTLRDLGVVMHEAGDPEAAERLHREAISLLEGALGPEAPAVAGTLGGLA
                     ELYIADGRLDEAAALLRRAADIWQAALDPDHPAVARSLSSLAMIELHRGRLDEAGPLQ
                     ERAVAILEAALGADHPDLATALGNLAMIRAGAGAPDQAAELYARAIAIRERAFGADSR
                     RVAVLLGGYAQALREGGHEPEAAAAEARAAALTPD"
     gene            complement(9855..11603)
                     /locus_tag="VGB88_04030"
     CDS             complement(9855..11603)
                     /locus_tag="VGB88_04030"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEX9646641.1"
                     /translation="MNMLGAEIVIRSLTDQGVEVVFGYPGGAVLPIYDALFQQNRLRH
                     VLVRHEQGAVHAAEGYARSTGRVGAVLVTSGPGATNAVTGLTDALMDSIPIVCLTGQV
                     PTHLIGNDAFQEADTVGITRPCTKHNYLVKRVEDLAPTIAEAFYVARSGRPGPVVVDL
                     PKDVVNASCDYLPVAGVEHKTYRPRLRADAERVAAAVDLVRAAKRPVIYAGGGVINSG
                     PRAAELLRELAHLTGQPVTQTLMGLGCFPASDPQSLGMLGMHGTYEANLAMHHCDMLL
                     AVGARFDDRVTGRLSDFSPGSKKIQIDIDPSSINKNVRVDVALVGDVAHVLADMVALW
                     KSRKLKPERAALKKWWQQIDQWRAVDCLNYEPSETTIKPQYALQRLQERTQGRDVYFT
                     TEVGQHQMWAAQFLKIEQPMRWMTSGGLGTMGYGLPAAIGVQVAHPEALVIDVAGEAS
                     FLMNMQELSTARQYRLPVKVFILNNEYLGMVRQWQEFFHGGRYAESYSDSLPDFVALA
                     EAFGMKGLRARTPGEVDGVIREMIDHDGPVIADICVAKHENVFPMIPAGAAHHEMKLS
                     AGDTVPPRAEEGMVLV"
     gene            complement(11663..12643)
                     /gene="miaA"
                     /locus_tag="VGB88_04035"
     CDS             complement(11663..12643)
                     /gene="miaA"
                     /locus_tag="VGB88_04035"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9646642.1"
                     /translation="MNAGIIVIAGPTAGGKSALALELAERLAADRLGAVLINADSMQV
                     YRELRVLSARPDAAAELRVPHRLYGILPASEPCSAARWRALALAEIAVARDAGRLAIV
                     VGGTGLYLHALLCGLAPVPEVPWAVRARARALYRELGADAFRARLAALDPDAAGRLAA
                     GDRLRLIRAWEVAEATGVTLAAWQARAPAPLGLPALKVLVMPAREALYRACDARFEAM
                     VAAGALAEVRALVAAGLDAELPAMKALGVPALARHLAGESTLEQAAEAAQRATRNYAK
                     RQLTWFRHRFAADLTLAPGPEAGLRLYRAVGCFLAGAAPGSPVDPAPRRD"
     gene            complement(12684..13244)
                     /locus_tag="VGB88_04040"
     CDS             complement(12684..13244)
                     /locus_tag="VGB88_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646643.1"
                     /translation="MKRQPPIPQRKLAFVGCEGDGELAYVALLNHIATARNLHIWFRA
                     EALNPGAGDHCAVVERAAARLAHRKRQGTRYLATAVLLDRDQWGTSKARDTRSVDVAK
                     RHGLLLVWQRPDHEALLLRHLDGCQALQPPATVSLNELKKRWPEYDKSGMTAARLDAR
                     VGYQQILQACTVEAELRQFLKRMGMI"
     gene            complement(13241..14455)
                     /locus_tag="VGB88_04045"
     CDS             complement(13241..14455)
                     /locus_tag="VGB88_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9646644.1"
                     /translation="MIYQLELENFYAIRERQVIDLRVAAKVPPEPHRFGPIYTGSPER
                     VPKVVAVFGANGSGKTTVLRALAFLVWFIRDSFHLKPEAPLPLEPFRSAESQAQVTRL
                     AVSLGGPTNLEGLNGSATTHGIYRYELQIAHRLGRSSAVISESLTHRLHGGRKPYRIF
                     LRTGDGKVVPGKSFSLTKYGSVVDKIRSNASVISTLAQFGHAPALALRDAAQSVTTNI
                     LIFKQEYTDAQVLEIYAGRPQLLEALNREIPRIDTGARQMHVLARPQGPLLEFDHEGL
                     SYPVPWLLESHGTQSFVRSFPLLWQPLANGGVAVIDELDASLHPLLLPEIARWFYDPR
                     RNPSNAQLWLTCQNASFLEELAKEEVLFCEKDNFGRSRVYGLQDIKYVRRVDNFYRKY
                     LSGTYGAVPNIG"
     gene            complement(14830..>16188)
                     /locus_tag="VGB88_04050"
     CDS             complement(14830..>16188)
                     /locus_tag="VGB88_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX9646645.1"
                     /translation="ADRLVPAVVNISTTQEVRGLGSGQAPMPQFPPGSPFEEFFRDFF
                     DRRGDEQQPAPQPRPRTTSLGSGFIVDASGYIVTNNHVIADAQEITVTLHDDSSYAAE
                     LVGHDPKTDVAVLKVVADKPLPAVQWGDSDSVKVGQWVVAIGNPFGLGNTVTAGIISA
                     RARDINAGPFDDFLQTDASINRGNSGGPLFNMNGEVIGINTAIYSPSGGSVGIGFAVP
                     ANLARNVVYQLQEFGETRRGWLGVRIQTVTDDLAESLGLDSARGALVASVSEESPAAA
                     AGLKVGDVILTFDGKDVQTMRRLPRIVAETEIGREVRVELWREGAPQTVGVTVGRLEE
                     GEVEVASAVQPPSEPLTESVPELGLKLSTITDDLRKEFQLGAEASGVMVVDVDAESVA
                     AEKGVRRGDVIVEVGQEEVASPADVQAKIKKHRELNKNTVLLLLDRQGDLQFVAVRLL
                     DS"
BASE COUNT         2354 a         5765 c         5579 g         2490 t
ORIGIN      
        1 gccgttggcc gccgagtaga gcatgaccag gccgaagccg gcgatcgcca ccaccagcaa
       61 gatcagcgcc cagttgatct ggacgacctt ctggccgagg ctcatctcgg gctggcgcag
      121 gtggccgatg gtggtcatcg cgggcttctc ccccgactca ggcccggccc ggatcggcgg
      181 gcgggcgggt cgcgaccggc ggcgccgtgc gcgccgagtc gcggccctgc gcctcgatca
      241 ggatgtcgcg caccatcggc gccgccaccg ccgagccgcc gccgccgtgc tcgaccacca
      301 ccgcgcaggc atagcgcggc gccgccaccg gggcgaaggc gatgaacagc gcgtggtcgc
      361 gctcggccca cggcttgtcc tcgttcttga ccacgccggt ctcgtgctcg cgcttggaga
      421 tgcgccgcac ctggctggtg ccggtcttgc ccgccatcgc cagcgcctcg tcctcgatgc
      481 gcgcgcgcca ggcggtcccg gtgcggtcat tgaccacctt gttcatgccc gcccggacga
      541 tctcaagcgc cgcggggtcg atgccgagca gcgcggcggc cggcgcggcc gcgggcgcct
      601 cggcgccgtc gtgtcgcaca tcgagggcga gcctcggcac caccgcccgc ccgccggcga
      661 tgcgcgcgca cgccactgca agctgcaacg gcgtcgtcag cacgtagcct tggccgatgc
      721 cggtgttcaa ggtctcgccc ttgtgccacg gctcgccgta ggtcgcgcgc ttccaggcct
      781 gggtcggcat cagccccgcc gcctcgcccg gcaggtcgag gccgagccgc gcgccgaggc
      841 cgagccggtg cgccatctcg gccaggcggt cgacgccgac cttgagcgcc acgtcgtaga
      901 aatacacgtc gcacgagcgc gagatcgcct cgaccatcgc caggcgcccg tggccgccct
      961 tcttccagca gtggaagcgc cggttgccga gctgcaaatg gcccgggcag taaacgctgt
     1021 ggccggggcc gagcaggccc gaatccagcg cggtcagcgc cgccaccacc ttgaaggtcg
     1081 agcccggcgg gtactggccg gcgatcgcct tgttgatcag cggcgcgcgc gggttctcga
     1141 ccagctcgtt ccactgcttg cgggtcaggc cctggttgaa caggttggga tcgaagccgg
     1201 gcgtggaggt cagcgccagc acctcgccgg tggcgatgtc gagcaccgcg gccgcggccg
     1261 actcggcgcc gaggcgggcg gcggcgaact cctgcaggcc gaggtcgagg gtcagcttga
     1321 catcgtcgcc cggccggccc tcctggcggc tgagctcgcg gatcacccgg ccgtgggcgt
     1381 tgacctcgac ctgctgggtg ccggcacgcc cgcgcaagct gaggtcgtgg atcttctcga
     1441 ccccgttctt gccgatccgg aagtcgggca gctcgagcag cgggtcgccg gtgagctcgc
     1501 tttccgacac cgccgcgaca tagcccagca catgtgcgag tgccgccgca tgcgggtatt
     1561 ggcggagctg gccgacctcg atcgagatgc ccggcagata gggcgcgttg atctcgatcg
     1621 cggccacctg gcgccacgac aggtgctcgc gcagcaccac cggcacgaag gcgcgcttgc
     1681 gcgcgatctc ggcgcgcacg cgctcgagct cgtcctcgcc gagcggcacg tagccgccga
     1741 ccgcggccaa ggtggcgtcc gtgtcgccgg cctgctcggg caccagcacc agccggtagt
     1801 tctggcgatt gaccgcgagc tcggcgccga agcggtcgaa gatgcgcccg cgcggcgggc
     1861 tcaggagctt gatgttgatg cggttgtcct cggccagcag gcggtactgg tggccctgga
     1921 cgacctggag gtagtaaagc ctcccgccga gcaccgacag gagcgcgagc ttgcccgcgg
     1981 cgagcagcac cgtgcgccgc gcgaacagcc gctggcgggc gcgttgctgc tggttcatcg
     2041 cgcccgcgcc tcgaggtcgc ccaacagctc gcgctggacc gtgccgagcg gccaggcgat
     2101 caccgggtac agcgccacgg tcaggatcgc ctggaacagg ctcgggctga tcggcatcgg
     2161 cctgagcgcg agcgccgagg cggcaagcca ccacagcgcc accgcgccgg tgccgatgac
     2221 ggcgaagccg agccacgccg ccggcagcgc cttggcggtg aagaaccgcc gctgggtccc
     2281 ggccaccgcc tgcagcacca gcaacaccag ggcggtcagg ccgaacggcg ccccggtcag
     2341 cgcgtcatgg accagcccga ccaacagggt cgcggtcatc ggcagcaggt cggggcggta
     2401 gacggtccag tagaacacgg cgatcgccgg cagcagcggc gtcaccgggg caaggccggg
     2461 cacgccgagc ggcaccgcgc acagcacggc gaggaacagg gtgagcacga acggcagcat
     2521 cgccctgagc tgcgactcga tgcggtgcca gagctcgttc tcttcggtca ttgtgccgat
     2581 gccccgccgc cctggccgga ggccatctcc ggcgcgcccg gcgcccccgc caccggctcc
     2641 tcgccgtcct cgcccgcctc gggcagcggc tcgaagcgcg gcccgtccca cgccaccagg
     2701 cggacgaact cgagccgtgc caggtccgcg gcgggctcga cgcgcacgcc ggcctcgccc
     2761 accgccacca ccaccccgac cggcaggccc ggcggcagca cgccgccgtg gcccgaggtc
     2821 acgacgcggg cgccgagcgc cggcgcgtac tcgagcggca ggtattcgag cctgagagtg
     2881 tccgagttgt cgccggcggc gatcgcgcgc tcgccgctgc cctcgataac caccggcacg
     2941 cgcgagttga ggtcggtgac cagcagcacc cgcgatgccc gccgcccgac ctcgaccacc
     3001 cgtcccgcca ggccctcgcc ggtgagcgcc gcctggccgg cggcgatgcc gtcgccggca
     3061 ccgacattga ccagcacggt gcgcacgaag gcgctggccg aatcgccgat cacccgcgcc
     3121 gagacgaacg ccggcttggc ctccagcctg agcccgagca gggctctcag cgccgcgttc
     3181 tcgtgctcga gccggcgggc cgcctgctcc cagcgcctga gccgggcaac cgcctcagtc
     3241 agctcggcgt tctcggtgcg cagcgtcagc aggccgcgca cctcggcgat catctgattg
     3301 accgtctcca ccggccgcga caggccgtcg agcaccggcg cgaacacgtc gaccaccgcc
     3361 gcgcgcaccc ggcccatggc ctcgggctcg gccttgccga ccaccatcag cgtcaccgac
     3421 aggccgatca cgagaagcag gccaaagcgg tgcagcagcg cgcggaccgg caccgcgacg
     3481 cggctgatgc tacccggcgg ccgccgctgc atgtcgtccg cccctggcct tagtcagtca
     3541 tgattggcac gctatccgat gactcccgag gaaacgccgt gcccccgttc ggtatgaagc
     3601 tcagtatgca ctgtgcagca cgtgcttcaa ggtccgcatc tcctcgagcg cacgcccggt
     3661 gccgagcgcg acgcaggtca gcgcctcctc ggcgatcgac accggcaggc cggtcgcctg
     3721 gcgcagcacg tggtcgaggt tgcgcagcag gccgccgccg ccggtgagca tgatgccctt
     3781 gtcgacgatg tcggcggcga gctccggcgg agtgttctcg agcgcgacct tgaccgtctc
     3841 gatgatcgcg ccgaccggct cggccaggct ctcggcgatc tggcgctcgc tgatcacgat
     3901 ctccttgggc acgccgttca tcaggtcgcg gcccttgatc tccatggtca cgccgttgcc
     3961 gtcctcgggc ggcatggcgc cgccgatggt catcttgatg cgctcggccg aggactcgcc
     4021 gatcaagagg ttgtggttgc ggcggatgta gccgatgatc gcctcgtcca tcttgtcgcc
     4081 gccgacgcgc accgacttgg cgtagacgat gccgccgagc gacagcaccg cgacctcggt
     4141 ggtgccaccg ccgacgtcga ccaccatcga gccggtcgcc tcggtcaccg gcaggccggc
     4201 gccgatcgcc gccgccatcg gctcctcgat caggtacacc cggcgcgcgc ccgcgctctc
     4261 cgccgactcc tggatcgcgc gctgctcgac cgcggtcgag cccgaaggca cgcagaccac
     4321 gatcagggga ctggcgaacg agcgccggtt gtgcaccttg cggatgaagt gcttgatcat
     4381 ctcctcggcg acctcgaagt cggcgatgac cccgtcgcgc agcggccgga tggcctcgat
     4441 gttgccgggc gtgcgcccga gcatctcctt ggcctcgtgg cccacggcca gcacctgctt
     4501 gcggccgcgc tggtcgatca gggccaccac cgagggctcg ttgagcacga tgccgcggcc
     4561 cgccacgtag accagcgtgt tggcggtgcc gaggtcgatg gccatgtcgt tcgacatcag
     4621 gcccatgagg cgcgagaaca tgttgttcta tttacactcc cgcactagcc gaaaaagacc
     4681 cgccagccac cttatcgcga gtcgtcgccg cgacgcggtg gcgtggtcgg ggtctcccta
     4741 aggtccgctc aggcacgcgc gaccagcgcc gccggcgcgc tcttctcgcg cttgaccagc
     4801 agcttgttga gcgcattcac gtaggcgcgg gcgctcgcga ccatggtatc ggtgtccgcg
     4861 ctctggccgt tgaccgagcg gccgttctcc tcgagccgca ccgagacctc ggcctgtgcg
     4921 tcggtgccct cggtcacggc gttgatctgg aacagctgca gcttggcgtg gtgcggaaac
     4981 agctcgccga tggcgcggaa caccgcgtcc accgggccgt cgccctcggc ctcggttgcg
     5041 ccctcgacgc cgtcgacctc gagggtcagt gcggcgcgcg ggcgcatgcc ggtgccgcag
     5101 tgcaccgcca gcgacaccag cttgatccgc tcatgctggt ggctgaccgc atcgtcgacc
     5161 agcgccacga tgtcctcgtc gtagatgtcc ttcttcttgt cggccaggtc cttgaaccgg
     5221 gcgaaggcgt cgttgagcgc gttgtcgccg acctcgtagc cgagctcgct gagcttggtc
     5281 ttgaacgcgt ggcgaccgga atgcttgccc atcaccaggg tcgacttggt caggccgacc
     5341 gactcgggag tcatgatctc gtaggtgccg gcgtgcttga gcatgccgtc ctgatggatg
     5401 cccgactcgt gggcgaaggc gttggcgccg acgatcgcct tgttgggctg gaccgtgaac
     5461 ccggtcaccg ccgagaccag ccgcgaggcg cgcatgatgt gctcggtgcg gatgttggtg
     5521 tggaacggca acaggtcgtt gcgggtgcgg atcgccatca cgatctcttc catcgccgca
     5581 ttgccggcgc gctcgccgag cccgttgacc gtgcactcga cctggcgcgc gccggcccgg
     5641 atcgcggcca gcgagttcgc caccgccagc cccaggtcgt tgtggcaatg gaccgagagc
     5701 acggcccggt cgatgttcgg cacgcgctcg aacagcatcc ggatcagcgc cgtgaactcc
     5761 tcgggcgtgg tgtagccgac tgtgtccggg acgttgatgg tgccggcgcc ggccttgatc
     5821 gcgctttcca ccgtccggca caggaagtcg tgctcggtgc gggtgccgtc ctcggggctc
     5881 cattcgacgt cgtcgcacag gttgcgcgcc cgggtgacgc tgtcgatcac cgcctggtga
     5941 acgtcctcgg gcgtcatctg aagcttgtgc ttcatgtgca gcgggctggt cgagatgaag
     6001 gtgtggatgc gcttgcgctt ggcgggcacc agcgcctcgg cggcgcggtc gatgtccttg
     6061 cgcccggcgc gcgccagcga gcacaccacc gacccggtga ccaccttgga gatctcgttg
     6121 atcgcctcga agtcgccctt cgaggcgatc gcgaacccgg cctcgatcac gtcgactccc
     6181 atctcctcga ggaccttggc gatctgcagc ttctcctcga gcgtcatgga cgcgcccggc
     6241 gactgctcgc cgtcgcgcat ggtggtgtcg aagatgatga cccgttcgcg gtcgtcggtt
     6301 cgtgtcatgg ctctgctctc ttggtacggg taatgggttg actcgggtac tcgactcccc
     6361 tgagcagccg ccaggacggc agctcagggg ccgctaagtc gaagcccgtt cccgcacagg
     6421 agcatgcgcg cacccgccgc gccggcctgg gggccgggcg cctcaatcga cgcatccagg
     6481 aagcggcggg tggtcggcat cggtcacgaa atggttcaga tgatcacagg ttaagataag
     6541 gtgccggcgg gccggcggca atgaccgcac cgccggctcg gcgtcgctcg ccaagcggat
     6601 tcatctttac gccccaaggc cggcgtcccg caaccggtat ttggcgcgca accgctcagt
     6661 tcttggtctt gtcgaccagg cggtgctcgg cgatccacgg catcatcgcg cgcagcttcg
     6721 cgccgacctc ctcgatcggg tgctgcgccg cgcgccggcg cagcgccttg aagctcggct
     6781 ggttgacctg gcactcgagc atccactcgc gggtgaagcg gcccgactgg atgtcgtcga
     6841 gcacccgctt catctcggcc cggacgtggt cgtcgatcag cctcgggccg cgggtgtagt
     6901 cgccgtattc ggcggtgttc gagatcgagt agcgcatgtt ggcgatgccg ccctcgtaga
     6961 tcaggtcgac gatcagcttg acctcgtgca ggcattcgaa ataggccatc tcgggcgcgt
     7021 agccggcctc gaccagggtc tcgtagccgg ccgcgatcag cgaggtgagg ccgccgcaca
     7081 gcaccacctg ctcgccgaac aggtcggtct cgcactcctc gcggaagctt gtctcgatca
     7141 ccccggcgcg tccgccgccg atcgccgagg cataggacag cgcgatctcg agcgcgttgc
     7201 ccgaggcgtc gcggtcgacc gcgaccaggc acggcacgcc gccgccgcgg acatattccg
     7261 agcgcaccgt atggcccggg cccttgggcg cgatcatgaa gatgtcgagg tcggcgcgcg
     7321 gctcgatcaa cccgaaatgg atgttgaggc cgtgggcgaa ggcgagtgcc gcgccatctt
     7381 tcatgttgcc ccggagatgg tcgcggtaga tgccggcctg gagctcgtcc ggggtcgcca
     7441 tcatcatcac gtcggcccac ttggcggcct cggtgacgcc catcaccttg aagcccgcgt
     7501 ccttggcctt ctttgccgag gccgagccct cgcgcagcgc caccgccacc tgggggacgc
     7561 cgctgtcgcg catgttggcg gcatgggcat gcccttggct gccgtagccg atgacgacca
     7621 cgttcttggc cttgatcagg ttgaggtcgg cgtcgcgatc gtaatagaca cgcatggtcg
     7681 gtttccttcc gggctgtggc gccggcggcc ggctcagccg agcctcattg ggggccgccc
     7741 gcgccgcggg caattgcggc ggcgccggtg cgcgccacct cggtcaggcc gagcgggcgc
     7801 atcagcgcga tgaacgcctc gatcttatcg gtcgtgccgg tcagctcgaa cacgaatgac
     7861 tcgatggtgc tgtcgaccac gcgcgcgcgg aacacgtcgg cgatgcgcag cgactcgacc
     7921 cgcttgtcgc cggtgccgca caccttgatc agcgccagct cgcgcgatac gtgtggtccg
     7981 ccctcggtca ggtcgatcac cgcgtgcacc ggcaccagcc ggccgagctg cgccttgatc
     8041 tgctcgatca ccatcggcgt gcccgaggtc accagcgtga tccgcgacag gttgcgctcg
     8101 ggatcgacct cggcgacggt caggctttcg atgttgtagc cccgccccga gaacaagccg
     8161 accacccgcg ccagcacccc gaactcgttg tcgacgatgc aggcgatggt atgggtctcg
     8221 atgatctcgc tcaccgccgc cccgtcgcgc gaattgaagt gaaagctgcc gggccccgaa
     8281 cgaggccggc actttagcga attgcgcttg attgtaaatg gcctagcccc cgcctaagct
     8341 gcccgcttgg cggccctgtt cgagacgcac accatgcccc gagcgctccg gttcctgatc
     8401 tgcgcgctgc tgcttgcagc ctggcccgcg gccgcccagc aagcgccgtc ggcggccgag
     8461 gtcggccgcg ccgaggcgcg caaccggacc accctcgagg ccttccaggc gggcgatgcc
     8521 gaggcggcgg tggcggcggc gcgcggcgcg gccgaggcca acctcgccgc gctcgggccg
     8581 ggccacgagc agaccgcgca cgcctggttc aacctcgcca ccatcctgtc gcggcagggc
     8641 gactacgacc aggccgaggc gctccaccgg cgcgcgctgg cggcccgcga ggcggcgctc
     8701 ggcgccgacc acgtcgacgt cgccgacagc ctgctcgcgc tcggccagct caaccgcaac
     8761 cgcggccact acgccgacgc cgagcagcag ttcgcgcgcg cgctcgcgat ccgcaccgcc
     8821 gcgctcggcg acggccaccc cgaggtcgcc caggtccacg gcgagctggc gcggctgtac
     8881 cgggtccagg accgcttcgc cgaggccgag gccaaccagg ccgcggcgct cgccatcttc
     8941 gagcgcgagc ttggccctga ccacccgctg gtcgccgacg cgctcagcga gctggcgctg
     9001 atccaccaca acgccggcgc ctacgaccag gccgagccct cctacctgcg ggttctcaag
     9061 atgcgcgaga cgcgcttcgg gcccgagcac gtgctggtgg cgcgcagcct cgccaacctc
     9121 ggcgcgctct accgcagcaa gggcgacctc gcccgcgccg aggactatta ccgccgcgcg
     9181 ctggcggcca agcgcgcggc gctcgggccc gcgcatccgg acgtcgcggt gaccttgcgc
     9241 gacctgggcg tggtgatgca cgaggcgggc gatcccgagg ccgccgagcg gctgcaccgc
     9301 gaggcgatct cgctgctcga aggcgcgctc gggcccgagg cgccggcggt ggcgggcaca
     9361 ctcggcggcc tggccgagct gtacatcgcc gacggccggc tggacgaggc cgcggcgctg
     9421 ctcaggcgcg ccgccgacat ctggcaggcg gcgctcgatc cggaccaccc ggcggtcgcc
     9481 cggtcgctgt cgagcctggc catgatcgag ctgcatcgcg gccgcctcga tgaggcgggg
     9541 ccgctgcagg agcgggcggt ggccattctc gaggccgcgc tcggcgccga ccatcccgac
     9601 ctcgccaccg ccctcggcaa cctggcgatg atccgcgccg gcgcgggcgc gcccgaccag
     9661 gcggcggagc tgtatgcgcg cgcgatcgcg atccgcgagc gcgcgttcgg cgccgacagc
     9721 cggcgggtgg cggtgctgct cggcggctac gcccaggcgc tgcgcgaggg cggccacgag
     9781 cccgaggcgg cggcggccga ggcgcgtgcc gccgcgctca ccccagatta ggggctttgg
     9841 cgccgccggc gccgctacac caggaccatg ccctcctcgg cgcgcggcgg cacggtgtcg
     9901 ccggcggaca gcttcatctc gtggtgcgcg gcgccggcgg ggatcatcgg gaacacgttc
     9961 tcgtgcttgg cgacgcagat gtcggcgatc accgggccat catggtcgat catctcgcgg
    10021 atcacgccgt cgacctcgcc aggcgtgcgc gcgcgcagtc ccttcatgcc gaacgcctcg
    10081 gcgagcgcca cgaaatcggg cagcgagtcg ctatagctct cggcgtagcg cccgccgtgg
    10141 aagaactcct gccactggcg caccatgccg agatactcgt tgttgaggat gaacaccttg
    10201 accggcagcc ggtactggcg cgcggtcgac agctcctgca tgttcatcag gaacgacgcc
    10261 tcgccggcga cgtcgatcac cagcgcctcg gggtgggcga cctggacccc gatcgcggcc
    10321 ggcaagccgt agcccatggt gccgagcccg cccgaggtca tccagcgcat cggctgctcg
    10381 atcttgagga actgcgccgc ccacatctgg tgctggccga cctcggtggt gaagtagaca
    10441 tcgcggccct gggtccgctc ctgcaggcgc tggagcgcgt attgcggctt gatcgtggtc
    10501 tcggacggct cgtagttcag gcagtcgacc gcgcgccact ggtcgatctg ctgccaccac
    10561 ttcttgagcg cggcgcgctc gggcttgagc ttgcgcgact tccacagcgc gaccatgtcg
    10621 gcgagcacgt gggccacgtc gccgaccagc gccacgtcga cgcgcacgtt cttgttgatc
    10681 gaggacgggt cgatgtcgat ctggatcttc ttggagccgg gcgagaagtc gctgagccgc
    10741 ccggtgaccc ggtcgtcgaa gcgcgcgccc accgccagca acatgtcgca gtgatgcatg
    10801 gcgaggttcg cctcgtaggt gccgtgcatg ccgagcatgc cgagcgactg agggtccgac
    10861 gccgggaagc agccgagccc catcaaggtc tgggtgaccg gctggccggt gaggtgggcg
    10921 agctcgcgca gcagctcggc ggcgcgcggg cccgagttga tcaccccgcc gccggcatag
    10981 atcaccggcc gcttggcggc gcgcaccagg tcgaccgccg ccgccacccg ctcggcgtcg
    11041 gcgcgcagcc gcggccggta ggtcttgtgc tcgaccccgg ccaccggcag gtagtcgcag
    11101 gaggcgttga ccacgtcctt gggcaggtcg accaccaccg gccccgggcg gcccgagcgg
    11161 gcgacgtaga acgcctcggc gatggtcggc gccaggtcct cgacccgctt gaccaggtag
    11221 ttgtgcttgg tgcacgggcg ggtgatgccg accgtgtcgg cctcctggaa ggcgtcgttg
    11281 ccgatcaggt gggtcggcac ctggccggtc aggcagacga tcgggatcga gtccatcagc
    11341 gcgtcggtga gcccggtcac ggcgttggtc gcgcccggcc ccgaggtcac cagcaccgcg
    11401 cccaccctgc cggtcgagcg ggcatagccc tcggccgcgt gcaccgcgcc ctgctcgtgg
    11461 cggaccagca cgtggcgcag ccggttctgc tggaacagcg cgtcgtagat cggcagcacc
    11521 gccccgcccg gatagccgaa tacgacctcc accccctggt cggtcaggga gcggatgacg
    11581 atttcggcac cgagcatgtt catggtcgcc tcccgtggct cgcccgcccg cggcgatcgc
    11641 cggcgggcag ggaccgcaac tcctagtccc ggcgcggggc cgggtcaacc ggcgagcccg
    11701 gcgccgcgcc ggcgaggaag cacccgaccg cacggtacag gcgcagcccc gcctcgggcc
    11761 ccggggcgag cgtgaggtcg gcggcgaacc ggtggcggaa ccaggtcagc tggcgcttgg
    11821 cgtagttgcg ggtcgcgcgc tgggcggcct cggccgcctg ctcgagcgtc gactcgccgg
    11881 ccaggtggcg ggcgagcgcc ggcacgccca gcgccttcat cgccggcagc tccgcatcga
    11941 gcccggcggc gaccagcgcc cgcacctcgg cgagcgcgcc ggccgcgacc atcgcctcga
    12001 agcgcgcatc gcaggcgcga tagagcgcct cgcgcgccgg catgaccagg accttcaagg
    12061 ccggcaggcc cagcggcgcg ggcgcgcgcg cctgccaggc ggcgagcgtc actccggtcg
    12121 cttccgccac ctcccaggcc cggatcaggc gcagccggtc gccggcggca aggcgcccgg
    12181 ccgcgtccgg gtcaagtgcg gcgaggcggg cgcggaacgc gtcggcgccg agctcgcgat
    12241 atagcgcgcg ggcgcgcgca cgcaccgccc aaggcacctc gggcaccggc gccaggccgc
    12301 acagcagggc gtgcaggtac aggccggtgc cgccgaccac gatcgccagc cggccggcat
    12361 cacgggccac ggcgatctcg gcaagggcga gcgcccgcca gcgggcggcg gagcacggct
    12421 cggatgcggg caggatgccg tacaggcggt gcggcaccct gagctcggcg gcggcgtccg
    12481 ggcgcgcgct cagcaccctg agctcgcgat agacctgcat gctgtcggca ttgatcagca
    12541 ccgcgccgag gcggtccgcc gccagccgct cggcgagctc gagcgccagc gccgacttgc
    12601 cgcccgcggt cgggccggcg atcacgatga tgcccgcgtt caccgccggc tccagccctg
    12661 ttcggacctg ttcccctgtg atcctagatc atgcccatcc gctttaggaa ctgcctcagc
    12721 tccgcctcga ccgtgcaggc ttgtaagatc tgttggtaac cgacgcgtgc gtcgagtctg
    12781 gccgcagtca ttccgctctt gtcgtattcg ggccatcgtt tcttgagctc gttcaggctc
    12841 actgttgccg gtggttgcag agcttggcaa ccatccagat gccgcagtag cagagcttca
    12901 tggtctggcc gctgccatac cagcaggagt ccgtgcctct tggcgacgtc gacggaacgc
    12961 gtgtcgcggg ccttcgatgt cccccattga tccctatcaa ggagcacggc cgtcgccaaa
    13021 tacctcgtcc cttgacgctt acgatgcgcg agacgagcgg ccgcacgttc gaccacggcg
    13081 cagtggtcgc cggcgccggg attgagtgct tcagcccgga accagatgtg gaggttgcgt
    13141 gccgtcgcga tgtgattcag caacgcgaca taggccagct cgccgtcgcc ctcgcaaccg
    13201 acgaaggcga gtttgcgttg tggaattggc ggctgccgct tcatccgatg ttgggaaccg
    13261 cgccataagt gcccgacagg tacttacgat agaagttgtc gacccggcgc acgtacttga
    13321 tgtcctggag cccatagacg cgcgaacggc caaagttgtc cttctcgcag aacaacactt
    13381 cctctttggc caattcctca agaaaggacg cattctggca tgtaagccac aactgtgcgt
    13441 tgctggggtt gcgtcgcggg tcgtagaacc atcgcgcgat ttccggcagg aggagcggat
    13501 gcaaggaggc gtcgagctca tcgatgaccg cgacgccacc gttggcgagc ggctgccaaa
    13561 gcagcggaaa cgaccgaacg aacgattgcg ttccgtggct ctcgagcagc cacggcaccg
    13621 ggtagcttag gccctcgtga tcgaactcca gcagcggccc ctgtgggcgt gctaagacgt
    13681 gcatttgccg ggcgccggta tcgatgcggg ggatttcccg gttgagtgcc tccagcaatt
    13741 gcggacgacc ggcatagatt tcgagtacct gggcgtcggt gtactcttgc ttgaagatga
    13801 gaatgttcgt cgtgaccgac tgggcggcgt cgcgcaacgc tagcgccggt gcatggccga
    13861 attgagccaa ggttgagatg acgctggcgt tggaacggat cttgtcgacg accgacccgt
    13921 atttcgtcag tgagaacgac ttgccgggca cgactttacc atcgccagtg cgcaggaata
    13981 tgcggtaggg cttccggccg ccgtgcaatc ggtgggtcag ggactcactg atgacggcgc
    14041 tggagcggcc cagccggtgc gcaatctgca attcgtagcg gtagattccg tgggttgtcg
    14101 ccgagccatt cagaccctcc agattggtgg ggccgccaag tgacacagcc agtcgtgtga
    14161 cttgcgcctg gctctcggcg ctgcggaacg gctcaagcgg cagcggcgcc tccggtttca
    14221 agtgaaagct gtccctgatg aaccaaacca ggaacgcgag cgcccgcagc accgtcgtct
    14281 taccggaccc gttcgcgcca aagactgcga cgaccttcgg cacgcgttcc ggcgatcccg
    14341 tatagatggg accgaagcgg tgcggctcgg gcggcacctt ggcggcgacc cgcaggtcga
    14401 tcacttggcg ctcgcgaatc gcgtagaagt tctcaagctc aagttggtag atcatgtggc
    14461 gttcttatcc gccgcaaggc cattacgtat caaggcgatt tcagcaaaaa atctctgaaa
    14521 acgcaagaaa acgaacggaa aggcgctcga tagcttgcaa atccgagcct agcagccctg
    14581 cagaagcgcg gtgtttctcg ggtcgcgagg gtcggtcttg cctaggtcaa cgccgtgtct
    14641 gccgcgtcac cagccgagtg agtccgacct gctattgggc cgccagctgg tgcccccgcg
    14701 cagggtctac acgagatctg gccggcgatc accactgcgc ccctggtcac gcgccgcccc
    14761 aagaaccgca agggggcccg ccggccccct tgccactggt cgcaaattcc gggccgggcc
    14821 gggccgggct cagctgtcga gcaggcgcac ggcgacgaac tggaggtcgc cctggcggtc
    14881 gagcagcagc agcacggtgt tcttgttcag ctcgcggtgc ttcttgatct tggcctgcac
    14941 gtcggccggg ctggccactt cctcctggcc gacctcgacg atcacgtcgc cgcggcgcac
    15001 gcccttctcg gcggcgacgc tctcggcgtc gacatcgacc accatcacgc cgctggcctc
    15061 ggcgccgagc tggaactcct tgcgcagatc gtcggtgatg gtcgacagct tgaggccgag
    15121 ctcgggcacc gactcggtca gcggctcgct cggcggctgg accgcggacg cgacctcgac
    15181 ctcgccctcc tcgagccggc cgacggtgac gccgaccgtc tgcggggcgc cctcgcgcca
    15241 cagctcgacc cggacctccc tgccgatctc ggtctcggcg acgatgcgcg gcaggcggcg
    15301 catggtctgc acgtccttgc cgtcgaaggt gagaatcacg tcgccgacct tgagtccggc
    15361 cgccgcggcc gggctctcct cgctgacgct ggcgaccagc gcgccgcgcg cgctgtcgag
    15421 gcccaggctt tccgccaggt cgtcggtgac cgtctggatg cgcacgccaa gccagccgcg
    15481 ccgcgtctcg ccgaactcct gcaactggta gacgacgttg cgggcaaggt tcgccggcac
    15541 cgcgaagccg atgccgacgc tgcccccgga cggcgagtag atggcggtgt tgatgccgat
    15601 cacctcgccg ttcatgttga acagcggccc gcccgagttg ccgcggttga tcgaggcgtc
    15661 ggtctgcagg aagtcgtcga acgggccggc gttgatgtcg cgcgcgcgcg ccgagatgat
    15721 gcccgcggtg acggtattgc cgagcccgaa cgggttgccg atcgcgacca cccactggcc
    15781 caccttgacg ctgtccgaat cgccccactg caccgccggc agcggcttgt cggcgaccac
    15841 cttgagcacc gcgacgtcgg tcttgggatc atggccgacc agctccgccg cgtacgagct
    15901 gtcgtcgtga agcgtgaccg tgatctcctg ggcgtcggcg atgacatggt tgttggtgac
    15961 gatgtagccg ctcgcatcga cgatgaagcc cgagccgagc gaggtcgtgc gcgggcgcgg
    16021 ctggggcgcc ggctgctgct cgtcgccgcg acggtcgaag aagtcgcgga agaactcctc
    16081 gaacggcgag cccggcggga actggggcat cggcgcctgg ccgctgccca ggccgcggac
    16141 ctcctgggtg gtcgagatat tcaccaccgc cggcaccaaa cggtcggc
//
LOCUS       DASWHU010000116         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_48765, whole genome shotgun sequence.
ACCESSION   DASWHU010000116 DASWHU010000000
VERSION     DASWHU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48765"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VGB88_04055"
     CDS             complement(<1..903)
                     /locus_tag="VGB88_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9646646.1"
                     /translation="MSLLTGGCAGTELAAHALKSAGAGQGSDRGKSGAGADYKLGEPY
                     VVDGVAYVPRDDPFYDNVGMASWYGTEFHGRRTANGDTYDMNALTAAHTTLPMPSKVR
                     VTNLDNGRELVVTVNDRGPFVDGRIIDVSRRAAQLLGFERQGTAKVRVRALAEQAPAD
                     KVYAGAAPPEVTVTEARADRGEAVAAVSSRVHGDVTMAAMAPPAPVPAAQAGGGFQLI
                     KSAAAAEPLPAVTAYYVQGGAFRDQDNAVRLERSLASFGPTRVIAVETGRQILYRVRL
                     GPIYAPETAQSLLHRVVAAGHPGAR"
     gene            complement(1007..1984)
                     /locus_tag="VGB88_04060"
     CDS             complement(1007..1984)
                     /locus_tag="VGB88_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9646647.1"
                     /translation="MVRAIAILSCCLAAAVVARPADAQRPMPFDDWVTGLRADALAAG
                     IGAATFDTAFAGIQPIARIIELDRSQPESRLTAAQYLDRVVSPERVAAARERLAGHRP
                     LLEEVAARYRVQPRFIVALWGIETDFGRFTGGFGVIEALATLAYDGRRADYFRGELLD
                     ALRILDQGHIEVSAMRGSWAGAMGQSQFMPSSFVAYAVDYDGDGRRDIWTSLADVFAS
                     IANYLASAGWRDDQTWGRAVRLPAGFDPALVDLAVVRPIGDWQALGVRRDDGAALPGR
                     PLPASIVRPDGPGAQAYLVYDNFRTTMKWNRSTYFALAVGRLADAIGEP"
BASE COUNT          343 a          817 c          840 g          309 t
ORIGIN      
        1 ccgcgcgccg ggatggccgg cggcgaccac ccggtggagc agggactggg cggtctccgg
       61 cgcgtagatc gggccgagac ggacccgata caggatctgc cggcccgtct cgaccgcgat
      121 gacccgggtc ggcccgaacg acgccaggct gcgctcgagg cggaccgcgt tgtcttggtc
      181 gcggaaggcg ccgccctgga cgtagtacgc ggtgaccgcg ggcaacggtt ccgccgccgc
      241 ggccgacttg atcagctgga acccgccgcc ggcttgggcg gcgggcacgg gcgcgggcgg
      301 cgccatcgcc gccatggtga cgtcgccgtg gacgcgagag gagaccgccg ccaccgcctc
      361 gccgcgatcg gcccgcgcct cggtgacggt cacctccggc ggggcggcgc cggcatagac
      421 cttgtcggcg ggagcctgct cggcgagcgc ccgcacccgc accttggcgg tgccttgcct
      481 ctcgaagccg agcagctggg cggcgcgccg cgagacgtcg atgatccgcc cgtcgacgaa
      541 cgggccgcgg tcgttgaccg tgaccaccag ctcacggccg ttgtcgagat tggtcacccg
      601 caccttgctc ggcatcggca aggtggtgtg cgccgcggtc agcgcgttca tgtcgtaggt
      661 gtcgccgttg gcggtacgcc ggccgtgaaa ctcggtcccg taccacgagg ccatgccgac
      721 attgtcgtag aacgggtcgt cgcgcggcac gtaggcgacg ccgtcgacaa cgtagggctc
      781 gccgagcttg tagtcggcgc cggcgccgga cttgccccgg tcggacccct gcccggctcc
      841 cgccgacttg agcgcgtggg cggcaagctc ggtgccggcg cagccgccgg tgagcaagct
      901 caccgccaga agcgcgagag tcagtggcgc gcggtatcgt cgcactgctc gctccccctc
      961 ctcgggcccg gccgtccccc gctaggacgg cccggaccta aatgacctag ggctcgccga
     1021 tcgcgtcggc aagccggccg acggcgagcg cgaaataggt cgagcggttc cacttcatgg
     1081 tggtgcggaa gttgtcgtag accaggtagg cctgcgcccc gggcccgtcg gggcggacga
     1141 tcgaggccgg cagcggccgt cccggaagcg cggcgccgtc gtcgcggcgc acgccgagcg
     1201 cctgccagtc gccgatcgga cgcaccaccg ccagatcgac cagcgccggg tcgaagccgg
     1261 ccggcaggcg caccgcccgc ccccaggtct ggtcgtcgcg ccagccggcg ctggccaggt
     1321 agttggcgat cgaggcgaac acgtcggcaa gcgaggtcca gatatcacgc cggccgtcgc
     1381 cgtcgtagtc gacggcgtag gcgacgaagc tcgacggcat gaactggctc tggcccatcg
     1441 ccccggccca tgacccgcgc atcgccgaga cctcgatgtg gccctgatcg aggatgcgca
     1501 gcgcgtccag cagctcgccc cggaagtagt cggcgcgccg gccgtcatag gcgagcgtcg
     1561 ccagcgcctc gatcacgccg aagccgccgg tgaagcggcc gaagtcggtt tcgatgcccc
     1621 acagcgcgac gatgaagcgc ggctgcacgc gatagcgcgc ggcgacctcc tcgagcaagg
     1681 ggcgatggcc ggcgaggcgc tcgcgcgccg ccgccacgcg ctcgggcgag accacgcggt
     1741 cgagatattg ggcggcggtg agccggctct ccggctggct gcggtcgagc tcgatgatgc
     1801 gcgcgatcgg ctggatgccg gcgaaggcgg tgtcgaacgt cgcggcgccg atcccggccg
     1861 cgagcgcatc ggcgcgcagg ccggtgaccc agtcgtcgaa cggcatcggc cgctgggcat
     1921 cggccggccg tgcaaccact gcagcggcga gacagcagct cagaatggcg atcgcccgga
     1981 ccatcggcct tcccgcaatc aatcacggcc gctcgatctt tgccacagca tcgatgtgcg
     2041 tacaatggcc gaggtcggca cggcgcagcg cgcccggtta tcacccgatt tctcaacaat
     2101 cggccaaact tgcccaggat catccggctg gcgggatgtc ctgtacttaa actagaatct
     2161 caagtagcat ccggccgctg cggcggcaac tgacacgaga ggcggcgatg cgcatcggcg
     2221 gcacgccctg gcgcaccatc tggctggccg aggacggctg cacggtcgag gtgatcgacc
     2281 agacccggct gccgtgggag gtcgcggtg
//
LOCUS       DASWHU010000117         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2635606, whole genome shotgun sequence.
ACCESSION   DASWHU010000117 DASWHU010000000
VERSION     DASWHU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635606"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VGB88_04065"
     CDS             <1..1639
                     /locus_tag="VGB88_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DM9 repeat-containing protein"
                     /protein_id="HEX9646648.1"
                     /translation="ARHGVLLVSAALAAAAYAPAPGHAMRDTEQRLLTDLLAAPAFRQ
                     RVPPHSAVTRLEFTGAVFPGDLVYGADPREIAVVPRALKNDLAVNCDRSQTRISGGTI
                     GGAYTNSSSFTTTSGWEIGTSLSFTANWPAASVNWTISGSVSGSTADMTGASETWEVS
                     QTWSAPLLPRTEMDVQMQVIQQKVDGQPYSMDLELRGKADIHYRPVLGWVDSTGGVPA
                     DAIIGGRETSPTSGRERRLPICRAGHRGAWHPGKVVAGNCNITWGGVEYEKRRFQVLT
                     GDSRLYSWRAYSGGGTDPENGVVAGTESRDDKHGGKLYVCRADYRGGTHPGKLVINDC
                     MIGYGGSEIQIGNYEILIFDKNQDRSFAINIEDYLPLERRSFRIEGQFEGVAALSTLT
                     LYSDSRPVSEARCPSAPVPAVRAAASERGAPLGFEAQRVPTAQIARAARAAEVALAPL
                     ARGTPLVETTVPSADPAAAAPAARLVSRLLRLERPNLFGADVLVMQQALNAAGWPMRA
                     DGFFGPRTDRALRHFQRANRLSADGIFGPRSQARMGL"
     gene            complement(1680..2207)
                     /locus_tag="VGB88_04070"
     CDS             complement(1680..2207)
                     /locus_tag="VGB88_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX9646649.1"
                     /translation="MTRIVAIDFETADYGADSACALGMARVVDGRIVATKAFLIRPPR
                     KRFAFTYIHGLTWADVEDAMTFAERIPEIERFVSGANYLAAHNAPFDRAVLAAGYAAA
                     RRRRPEVPFLCTVRMARAAWGVRPTKLPDVCRYLGIALRHHDAASDALACARIAAAAI
                     ADGFPIETSRLRSPT"
     gene            complement(2216..2911)
                     /locus_tag="VGB88_04075"
     CDS             complement(2216..2911)
                     /locus_tag="VGB88_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646650.1"
                     /translation="MADKQGALIVGAGDGLSASLARLFAREGYAIALAARRIGKLDRL
                     CQETGAQAQACDAAEPDQVAALFAWLDDTLGVPAVVAYNPSFRTRGPLVELDAGDVAK
                     ALAITAFGGFLVGQQAARRMIEAGGGAILFTGASASVKGYAGSAPFAMGKFALRGLAQ
                     SMARELQPKNIHVAHFVIDGAIGNPGVRTPAGQADSLLDPDAIARSYLDIVRQHRSAW
                     TWEVELRPWVERF"
     gene            3021..>3601
                     /locus_tag="VGB88_04080"
     CDS             3021..>3601
                     /locus_tag="VGB88_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9646651.1"
                     /translation="MLDRRSFLGLGAAATVGLGGVEVLAGRGEAGGEAPGIRSYVTLG
                     RTGLTISDISFGSSHSADPALVRHALTRGITYFDTAESYRFGTAEEAIGEALQGKRDR
                     VVLSSKTKADTGDRRTDMMRALEGSLERLGTDHVDIYFNHAVNDVDRMQNQEWWEFTE
                     LAKRQGKIRFRGMSGHGSRLPECLDYAIDHDLV"
BASE COUNT          535 a         1299 c         1289 g          478 t
ORIGIN      
        1 ggcacggcac ggcgtcctgc tcgtgtcggc ggcgttggcg gcggcggctt acgcgcccgc
       61 gccgggccac gccatgcgcg acaccgagca acggctgctc acggaccttc tcgccgcccc
      121 tgccttccgc cagcgcgtgc cgccgcacag cgccgtcacc cggctcgagt tcaccggcgc
      181 cgtgtttccc ggcgatctcg tctacggcgc cgacccgcgc gagatcgcgg tggtgccgcg
      241 ggcgctgaag aacgacctcg ccgtcaactg cgaccgcagc cagacgcgca tctccggtgg
      301 caccatcggc ggcgcctaca cgaattcgtc atccttcacc accaccagcg gctgggagat
      361 cggcacctcg ctgtcgttca ccgccaactg gccggccgcc tcggtgaact ggaccatctc
      421 cggcagcgtc agcggctcga ccgccgacat gaccggcgcg agcgagacct gggaggtgtc
      481 gcagacctgg agcgcgccgc tgctgccgcg caccgagatg gacgttcaga tgcaggtgat
      541 ccagcagaag gtcgacggcc agccctacag catggacctc gagctgcgtg gcaaggccga
      601 cattcactac cgcccggtgc tcggctgggt ggactcgacc ggcggcgtcc ccgccgacgc
      661 gatcatcggt gggcgcgaga cctcgccgac cagcgggcgc gagcggcggc tgccgatctg
      721 ccgcgccggc caccgcggcg cttggcatcc gggcaaggta gttgccggca attgcaacat
      781 cacctggggc ggcgtcgagt acgagaagcg ccgcttccag gtgctgaccg gcgacagccg
      841 gctgtacagc tggcgcgcct acagcggcgg cggcaccgac ccggaaaacg gcgtcgtcgc
      901 cggcaccgag tcccgcgacg acaagcatgg cggcaagctc tatgtctgcc gcgccgacta
      961 ccgcggcggc acccaccccg gcaagctggt catcaacgac tgcatgatcg gctacggcgg
     1021 cagtgagatc cagatcggca attatgagat cctgatcttc gacaagaatc aggatcgcag
     1081 ctttgcgatc aatatcgagg actatctgcc gctcgagcgg cggtcgttcc gcatcgaggg
     1141 ccagttcgag ggcgtcgccg ccctgtcgac gctgaccctc tacagcgatt cgcgcccggt
     1201 gagcgaagcg cgctgcccgt cggcgcccgt gccggcggtg cgcgcggcgg caagcgaacg
     1261 gggggcgccg ctgggcttcg aggcgcagcg cgtcccgacg gcacagatcg cccgtgccgc
     1321 gcgcgcagcc gaggttgcgc tcgccccgct cgcccggggg acgccgctgg tcgagacgac
     1381 cgtgccgagc gccgacccgg cggcggcggc gcccgcggcg cgcctggtct cgcgcctgct
     1441 ccggctcgag cgaccgaacc tgttcggcgc cgacgtgctg gtcatgcagc aggcgctgaa
     1501 cgcggccggc tggccgatgc gcgcggacgg cttcttcggg ccacggaccg accgcgcgct
     1561 caggcatttc cagcgcgcca accggctgtc cgccgacggc atcttcggcc cccgcagcca
     1621 ggcgcggatg ggactgtagc gcgggcgcgc gaacggccgt ggcgcgcgcg ccggttccgt
     1681 caggtcggcg aacgcaggcg gctggtctcg atcgggaagc cgtcggcgat cgcggcggcg
     1741 gcgatccgcg cgcaggcgag ggcgtccgag gcggcgtcgt ggtggcgcag cgcgatgccg
     1801 agataccggc acacgtcggg cagcttggtc gggcgcacgc cccacgccgc gcgcgccatc
     1861 cgcaccgtgc acaggaacgg cacctcgggc cgccgccgcc gcgccgccgc atagcccgcg
     1921 gcgagcaccg cccggtcgaa gggcgcatta tgagccgcca gatagttggc acccgaaaca
     1981 aacctctcga tctcggggat gcgctcggcg aaggtcatcg cgtcctcaac gtccgcccag
     2041 gtcaggccat ggatgtaggt gaacgcgaag cgcttgcgcg gcgggcggat caggaacgcc
     2101 ttggtcgcga cgatgcggcc gtcgacgacc cgtgccatgc ccagcgcgca ggcgctgtcg
     2161 gcgccgtagt cggcggtctc gaagtcgatg gcgacgatcc tggtcacgcg gcgcgtcaga
     2221 agcgctcgac ccagggcctg agctcgacct cccaggtcca ggcgctgcgg tgctggcgga
     2281 cgatgtcgag atagctccgc gcgatcgcgt cgggatcgag caggctgtcg gcctggccgg
     2341 cgggcgtgcg cacgccgggg ttgccgatcg cgccgtcgat gacgaagtgg gcgacgtgga
     2401 tgttcttggg ctggagctcg cgcgccatgc tctgggcgag gccgcgcagc gcgaacttgc
     2461 ccatcgcgaa cggcgccgag ccggcgtagc ccttgacgct cgccgaggcg ccggtgaaca
     2521 ggatcgcgcc gccgcccgcc tcgatcatcc gccgcgccgc ctgctggccg accaggaagc
     2581 cgccgaacgc ggtgatcgcg agcgccttgg cgacgtcgcc ggcatcgagc tcgacgagcg
     2641 gcccgcgggt gcggaagctc gggttgtagg ccaccaccgc gggcacgccg agcgtatcgt
     2701 caagccacgc gaacagcgcc gcgacctggt cgggctcggc ggcatcgcac gcctgcgcct
     2761 gcgcaccggt ctcctggcac agccggtcga gcttaccgat gcgccgcgcg gcgagcgcga
     2821 tcgcgtagcc ctcgcgcgcg aacagccgcg ccagcgaggc actgaggccg tcgcccgcgc
     2881 cgacgatgag cgcgccctgc ttgtccgcca tgacctgccg tcaccttccg cggccattag
     2941 actcgaccgg cgggcattga acaagcgcgg cgcgccccgt accatggcct tgcgaaggcc
     3001 gttcgagggg cgagtccgag atgctcgacc gacgcagctt cctgggcctc ggcgcggcgg
     3061 cgaccgtcgg gctcggcggc gtcgaggtgc tggccggccg cggcgaggcc ggcggcgagg
     3121 cgccgggcat ccgcagctac gtcaccctcg ggcgcaccgg gctcacgatc tcggacatct
     3181 cgttcggctc gagccactcc gccgaccccg cgctggtacg ccacgcgctc acccgcggca
     3241 tcacctattt cgacaccgcc gagagctacc gcttcggtac cgccgaggag gcgatcggcg
     3301 aagcgctgca gggcaagcgc gatcgggtgg tgctgtccag caagaccaag gcggacacgg
     3361 gcgaccggcg cacggacatg atgcgcgcgc tggagggcag tctcgagcgc ttgggcaccg
     3421 accatgtgga catctacttc aaccacgcgg tcaacgacgt ggaccgcatg cagaaccagg
     3481 agtggtggga gttcaccgag ctggccaagc ggcagggcaa gattcgcttc cgcggcatgt
     3541 cgggccacgg cagccggctg ccggagtgcc tggactacgc catcgatcac gatctggtgg
     3601 a
//
LOCUS       DASWHU010000118        14183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1528174, whole genome shotgun sequence.
ACCESSION   DASWHU010000118 DASWHU010000000
VERSION     DASWHU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528174"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VGB88_04085"
     CDS             complement(<1..142)
                     /locus_tag="VGB88_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9646652.1"
                     /translation="MIPRYSRPEMAAIWAPETRFRIWLDIERFACEAQEQLGVIPRGV
                     AKA"
     gene            complement(328..2379)
                     /locus_tag="VGB88_04090"
     CDS             complement(328..2379)
                     /locus_tag="VGB88_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEX9646653.1"
                     /translation="MDNLWSAAAARAAVARYAAQGIGADLALRVYTTRLLGGEPRLVQ
                     HGGGNTSVKTRLTDLVGEEVEALCVKGSGWDMATIEPAGLPAMRLAAVRPLLALDALS
                     DEDMVNAQRASLLDSTAPNPSVEALLHAFLPHKFVDHTHAAAVLALTDQPAGAAICAE
                     VYGERAALVPYVMPGFALAKAAHETLREHPNVEGLILLKHGIFTFADTAEEAYRRMIE
                     LVSLAEARIAAAGRRLLAGAALPPAVAPLAEVAPILRGLASRPPGGADEPVRWMLEAR
                     TGPEILAYVSGAELARYARAGVVTPDHAIRTKGWPLILPAPEAGALGSFHDDAARAFA
                     AFRDDYDSYFERNDAAQATRRKRLDAVPRVILVPGLGLFALGKSAKEARIAADLAETT
                     VRVVTDAEAVGRFESIGERDLFDIEYWSLEQAKLGKATERRLARHVLVITGAAGAIGA
                     ACARAFAAEGAEVAALDRDEAGAQSVAAEVGGLGLGCDVTESHAVRAAFARVCETFGG
                     LDIVVSNAGAAWQGRIGEVDEAVLRQSFELNFFAHQSVAQAAVRVMRAQGIGGVLLFN
                     ASKQALNPGRDFGPYGLPKAATLALSRQYALDHGGEGIRANAVNADRIRSGLLTEDMI
                     AARAAARGVSEATYMAGNLLGREVTAADVAEAFVALAVARKTTGAVLTVDGGNIAAAP
                     R"
     gene            2678..3178
                     /locus_tag="VGB88_04095"
     CDS             2678..3178
                     /locus_tag="VGB88_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9646654.1"
                     /translation="MFNKTDDPQDAAGPSQAHPTRATNSGGKADQSQISAGLKVVGNL
                     ESDGDVVISGVVEGDIASRGLTIAQGATVQGSIQADNVQIMGSVQGQVRARTVKIART
                     GEMTGDVNYESLSIEEGAMLDGQCRRIQSGRAAGDAKVSKLTAVEGGAKKEEAKPSGT
                     GGSKAV"
     gene            3266..3622
                     /locus_tag="VGB88_04100"
     CDS             3266..3622
                     /locus_tag="VGB88_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9646655.1"
                     /translation="MPAADGEPALIAHGLRITGSMTGNVDVVIDGALAGEVRGRIVTI
                     GPRADVDGSLKAHAVDIAGRMQGRVEAMLVHIGPGAEVDATIYHHKLDVERGAVVRGL
                     RPWRPLSDMERRRSAW"
     gene            3616..4191
                     /locus_tag="VGB88_04105"
     CDS             3616..4191
                     /locus_tag="VGB88_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9646656.1"
                     /translation="MVRPRAFAEAEPRLGAGRRAGLGLAAALALLFAAIAPAQAGEAA
                     APGPCLGPAVGVAVPLCRQIAAHQPNGPWIPSGRRLTYASGRPREGEVRRLDRFGLEV
                     AEITGALAARYRITQSVAGVVVVGIAPAGPAAGKGLAVGDVIVELDQQPVAGPGDLSR
                     RLEAVGRAPATSALLLLYRDYEHRFQVLALD"
     gene            complement(4204..4917)
                     /gene="radC"
                     /locus_tag="VGB88_04110"
     CDS             complement(4204..4917)
                     /gene="radC"
                     /locus_tag="VGB88_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX9646657.1"
                     /translation="MAGNDAEERMRVVPRATAAGHRQRLRKRFLAQGGESMADYELLE
                     LALFAALPRRDVKPLAKSLLARFGTFAEVIGAEPARLRDVRGVDEAVVATLKVIEASA
                     HRMLRDRILDRPVIASWAALLDYCAAAMQRNATEQFRVLFLDRKNVLIADELQQRGTV
                     DHAPVYPREVVRRALDLGASALILVHNHPSGDPTPSRQDIDTTRQVVEAAKALGIAVH
                     DHLVIGRKGHASFKTLGLL"
     gene            complement(4940..5758)
                     /gene="map"
                     /locus_tag="VGB88_04115"
     CDS             complement(4940..5758)
                     /gene="map"
                     /locus_tag="VGB88_04115"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9646658.1"
                     /translation="MDDNDWTSGPGRSVKIHGPEAFEGMRSAGRLAAEVLDFIAPQVV
                     AGVTTGELDRLCHDFIVDHGAVPAPLNYRGFPRSICTSVNHVVCHGIPDERKRLQPGD
                     IVNIDITVILDGWHGDTSRMFWLDSVPRKAARLTDVTFEAMWCGIRAVRPGATLGDIG
                     HAIQAYAENHHCSVVRDFCGHGIGQVFHDAPSVLHYGRPGAGLALREGMFFTIEPMIN
                     LGRYETKILADGWTAVTKDRSLSAQFEHTVAVTADGHEVFTASPAGLDRPPYTA"
     gene            6129..6893
                     /locus_tag="VGB88_04120"
     CDS             6129..6893
                     /locus_tag="VGB88_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9646659.1"
                     /translation="MNQAASSQPTACVLIIGNEILSGRTQDVNLNYIAKRLAECGVRL
                     AEARVIPDQEAAIIDAVNACRAAYDWVFTTGGIGPTHDDITAECIAKAFGVGLEQNAE
                     ARALLERQYRERNVELNEARLRMANMPAGALLVDNPVSGAPGFQVGNVFVLAGVPSIM
                     QAMFESAVEHIPGGVPLSSRTVVAYLPEGTLAGPLKELQGEYPDIDMGSYPFYRVGRF
                     GSNLVLRAADEGRLEEAVNKLVACVDRLGGEPQLEG"
     gene            7063..7620
                     /locus_tag="VGB88_04125"
     CDS             7063..7620
                     /locus_tag="VGB88_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX9646660.1"
                     /translation="MQVPIEVSFKNMDSSEAIAADIRQRVAKLDHFFEHVTGCHVSVE
                     APHKHHRKGRHWRVRIVINAPGRQLVVSNEPGDLNAHEQLAVALRDAFNAATRQLQDH
                     ARTMRGDVKSHQPAQVEGSIARVFADQGYGFIDAGPLGEVYFHRHSVVGDRFAELAAG
                     SRVRFVVAEGESEHGAQASTVHPLD"
     gene            complement(7917..8186)
                     /locus_tag="VGB88_04130"
                     /pseudo
     CDS             complement(7917..8186)
                     /locus_tag="VGB88_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8455..9762)
                     /locus_tag="VGB88_04135"
     CDS             complement(8455..9762)
                     /locus_tag="VGB88_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9646661.1"
                     /translation="MIDVARGAPLPGATVVVEDGLITAVGADTAVPGSARVVALAGKW
                     LIPGLIDGHVHLFQSGGLYARPDVIDLRAVVPYEAEIARVRSDLDQTLARIFAAGVTG
                     ILDLGGPAWVAPLRARAEAAAMPAPRIAWAGQLLGSWSPAALAAAEPAVVLVETPEQA
                     RAEVRRQAGLGADLIKVWFIQALGDQDFATTRRWVIAAIDEAHALGLGAAVHATELAT
                     AKAALAAGADLLVHSVDDAAVDDAFLAAMRARDVPYVPTLGVYDGYWRVLRQKLALTD
                     LDRRYGARRVLDSLDDLAALPRETLPAWLDFDQPVPPVNQVMIDNLARVHAAGVTVVA
                     GSDSGNIGTVHGAALHKELRLMARAGLSPAEILRSATLAGARAMGRGEELGLIAPGRR
                     ADMVALDADPLADTANLARIHRVIKDGRVWDPDEVLERTRSDD"
     gene            10327..11721
                     /locus_tag="VGB88_04140"
     CDS             10327..11721
                     /locus_tag="VGB88_04140"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX9646662.1"
                     /translation="MPFHPGIVSGLATLADGASVFGTGLVVYMLYVGWQGHSLPLYLS
                     FLTLDTLFVLAAFGLAGLYHFESLSHPERQLHKIFMICGLSFMIMTVLAFALKISEEF
                     SRVWAFAWFFAATAGICLVRVFGHAQLSEWAVKGRLTRNVAVVGAGIQGQRLVEALQA
                     SEDPWLNVIGVFDDRTTRVPSEVGKVPVIGGLGQLVDFVRQNRIDDIVIALPWSAESR
                     QLGIVEKLSLLPAHVQLAPDMIGFHFPVSRYDRLGAVSCLKVSARPLDGWSAVLKAVE
                     DWLVASVILFAVLPLMLMIAAAIKLGSRGPVFYRQERYGFNNQLIEVFKFRTMYDDPD
                     PDPEVLQARRNDPRVTRVGAFLRRTSLDELPQLLNVLRGEMSVVGPRPHAVAHDEKYG
                     RVIDRYLGRFRVRPGITGWAQVNGWRGETDTLAKMRARIEHDLSYIENWSVALDLRIL
                     LWTVGIVVRGRNAY"
     gene            11877..13400
                     /gene="xrtD"
                     /locus_tag="VGB88_04145"
     CDS             11877..13400
                     /gene="xrtD"
                     /locus_tag="VGB88_04145"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HEX9646663.1"
                     /translation="MGAAGLLLILASWDGLVEMAQSWSREEYSHGYLIPIVVAFLLAR
                     GLARAEAVPEGRRWPGPAIVALGGAMAVLGQASMIRDLSIYGFFVGLVGLAVSVLGHR
                     RVVKVWAPVAYLLFMVPLPQFVYLKLSAEMQLLSSELGAGMVRMLGIPVFLDGNVIDL
                     GVYTLQVAEACSGLRYLFPLMSFGFLFAMLFSGARWQKVVLFLSSLPIAVIMNSVRIA
                     VIAVLANDYGIAEAEGFLHFFEGWVIFLACVALLFAAAWLLDRLGRWAGRQRAAIDLR
                     PPDARALRRLAGSIRGVSATLVASTVLALSAGVGQLAPFGDAHANLVRDDLSAFPAEI
                     GTWRGQPEALEREIRQVLAADDYLVSGFVANDSGAPVSLFIAYYATQTDGRAAHSPEI
                     CIPGGGWEIVSLSSRSVDLESASEAGLRVNRAIIQKGRYRQLVYYWFEQRGRRLVNEY
                     VVKWYILWDALTRKRTDGALVRLVTPLIIGEDEPAADARLARFLRGAYPVFGRFIPA"
     gene            complement(13491..>14183)
                     /locus_tag="VGB88_04150"
     CDS             complement(13491..>14183)
                     /locus_tag="VGB88_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9646664.1"
                     /translation="LLVLLGDVESQLGDGDAAIQAYRRAVELAVSTPSAYLRLAQLYA
                     KRETLDQAIDVLQAAHASNPGVEDIGLALGMALQNAERFTEAIALYEQILESSPRSTV
                     AANNLAALIADHDNQDTTRLQRALDLAEGFQTSDNPLLIDTLGWAHYRLGSISQAILF
                     LKQAVQLSPSNAELNYHLGMAYLADNQTESARAYLTNALGDGGTSYFTADVRQVLAEL
                     DARTDGKEGAGP"
BASE COUNT         2050 a         4836 c         5068 g         2229 t
ORIGIN      
        1 gcgccttggc cacgccgcgc gggatcacgc cgagctgctc ctgcgcttcg caggcgaacc
       61 tttcgatgtc gagccagatc cggaagcgcg tctcgggcgc ccagatggcg gccatctccg
      121 gccggctgta gcgggggatc atcgtgcgca ccagcactcg atcgagcacg agctgcaggc
      181 acgcaacggc ctcgcgcttc gagacggccc tgcgggcctc ctcagcgtga ggccgttgta
      241 ctccaacgag aaacctcgtc ctgaggagcg agcggagcga gcgtctcgaa ggacggcgac
      301 cggctcagcc atctggcatt cccgaaccta gcgcggcgcc gcggcgatgt tgccgccgtc
      361 gacggtgagc accgcgccgg tggtcttcct ggccacggcg agggcgacga acgcctcggc
      421 gacgtcggcc gcggtcacct cgcgcccgag caggttgccg gccatgtagg tcgcctcgct
      481 caccccgcgc gccgccgccc gcgcagctat catgtcctcg gtcaggaggc ccgagcggat
      541 gcggtcggcg ttgaccgcgt tggcgcggat tccttcgccg ccgtggtcga gcgcgtattg
      601 gcgggacagc gccagggtcg ccgccttggg cagcccgtag gggccgaagt cgcgccctgg
      661 gttgagcgcc tgcttcgagg cgttgaacag cagcaccccg ccgatgccct gcgcgcgcat
      721 cacccgcacc gccgcctggg cgacggactg atgggcgaag aagttgagct cgaagctctg
      781 gcgcagcacc gcctcgtcga cctcgccgat gcgcccctgc cacgccgcgc cggcgttgga
      841 gacgacgatg tcgaggccgc caaaggtctc gcagacgcgc gcgaacgcgg cgcgcaccgc
      901 gtgcgactcg gtgacgtcgc agccgaggcc gagcccgccg acctcggccg ccacactctg
      961 cgcgccggcc tcgtcgcggt cgagcgccgc cacctcggcg ccctcggcgg caaaggcgcg
     1021 cgcgcacgcc gcgccgatcg cgcccgcggc gccggtgatc acgagcacgt ggcgggccag
     1081 gcgcctctcg gttgccttgc cgagcttcgc ctgctcgagc gaccaatatt cgatgtcgaa
     1141 caggtcgcgc tcgccgatgc tctcgaatct gccgaccgcc tcggcgtcgg tgaccacgcg
     1201 cacggtggtc tcggcgaggt cggcggcaat gcgcgcctcc tttgccgact tgccaagcgc
     1261 gaacaggccg agaccgggca ccaggatcac ccgcggcacc gcatcgaggc gcttgcgccg
     1321 tgtcgcctgg gcggcgtcgt tgcgctcgaa ataggagtcg tagtcgtcgc ggaaggcggc
     1381 gaaggcgcgc gcggcgtcgt cgtggaagct gccgagcgct ccggcctcgg gcgccggcag
     1441 gatcagcggc cagcccttgg tgcggatggc gtggtcgggg gtgaccacgc ccgcgcgggc
     1501 gtagcgggcg agctcggcac cgctgacgta agcaaggatc tcaggcccgg tgcgcgcctc
     1561 cagcatccag cgcacgggct cgtccgcgcc gccgggaggc cggctcgcca gcccgcgcag
     1621 gatcggcgcg acctccgcga gcggcgccac cgccggcggc agcgcggcac cggcgagcag
     1681 cctgcgcccg gcggccgcaa tccgcgcctc ggccagtgac accagctcga tcatccgccg
     1741 gtacgcctcc tcggcggtgt cggcgaaggt gaagatgccg tgcttgagca aaatcagccc
     1801 ctcgacgttt gggtgctctc tcagcgtctc gtgcgccgcc ttggcaagcg cgaatccggg
     1861 catcacgtaa ggcaccagcg ccgcgcgctc gccatagacc tcggcgcaga tcgccgcgcc
     1921 cgcgggctgg tcggtcagcg ccagcaccgc cgcggcgtgg gtatggtcga cgaacttgtg
     1981 cggcaggaac gcgtgcagca gcgcttccac cgacgggttc ggcgcggtgc tgtcgagcag
     2041 gctcgcgcgc tgggcgttga ccatgtcctc gtccgacagc gcgtccagcg cgagcagcgg
     2101 gcggaccgcg gcaaggcgca tggccggcag ccccgccggc tcgatggtcg ccatgtccca
     2161 gccgctgccc ttgacgcaca gcgcctcgac ctcctcgccg accaggtcgg tgaggcgcgt
     2221 cttgaccgag gtgttgccgc cgccgtgctg gaccaggcgc ggctcgccgc cgagcaggcg
     2281 cgtggtgtag accctgagcg ccaggtccgc gccgatgccc tgcgcggcgt agcgcgcgac
     2341 cgccgcgcgc gccgccgccg ccgaccacag gttgtccatg ccgccggcct ccgctcgcgc
     2401 gccggcgctc ggcgcgcgcc aaaccgagcc ctcaataggg ccgcgccgcg agccggtcaa
     2461 ccggcgcctc gggcggatgc ggactcgccg gcccgatctc ggatcgccgt cgaccgggcg
     2521 ggtgattcgg tgctgcgccg tgcggcctcg ccgctgttgc gcggccggcg gcgggcctct
     2581 gcggtcagtg catggccggc ggctggaacg gcgcttgcgt tggcgaccgg tcggggctct
     2641 ataatcgcgc ggggactggt tcaggaggac aagccacatg tttaacaaga ccgacgaccc
     2701 gcaggacgct gcgggaccat cccaggcgca cccaacccgc gccaccaact ccgggggcaa
     2761 ggccgatcag tcgcagatca gcgccggcct gaaggtggtc ggcaacctgg aaagcgacgg
     2821 tgacgtggtg atcagcggcg tcgtcgaggg cgacatcgcc agccgcgggc tgaccatcgc
     2881 acagggcgcc accgtccagg gctcgatcca ggccgacaac gtccagatca tgggctcggt
     2941 ccagggccag gtgcgggcgc gcacggtcaa gatcgcgcgc accggcgaga tgaccggcga
     3001 cgtgaactac gagtcgctga gcatcgagga gggagcgatg ctcgacggcc agtgccggcg
     3061 catccagtcc ggtcgcgccg ccggggatgc caaggtctcc aagctgaccg cggtcgaggg
     3121 cggggccaag aaggaggagg ccaagccatc cggcacgggc ggctcgaagg cggtctgagg
     3181 ccgcgctcac cgcggcagct atcgatccgg gcggcacatg cgactcagtc tgtccggcgc
     3241 tccggccacc aacggtgcgg gcgggatgcc cgctgccgac ggtgagccgg cgttaatcgc
     3301 ccatggcctc aggatcacgg gctcgatgac cggcaatgtc gatgtggtca tcgacggcgc
     3361 cttggccggc gaggtgcgcg ggcggatagt caccatcggc ccgcgcgccg acgtcgacgg
     3421 ctcgctgaag gcgcacgcgg tcgacatcgc gggacggatg caggggcggg tcgaggcgat
     3481 gttggtgcac atcggccccg gcgccgaggt cgacgccacc atctatcacc acaagctcga
     3541 cgtcgagcgg ggggcggtgg tgcgaggcct gcggccgtgg cggccgctgt ccgacatgga
     3601 acgccggcgc tcggcgtggt gaggccgcgg gccttcgccg aagcggagcc tcggcttggc
     3661 gccggcaggc gggccggcct cggcttggcg gcggcgctgg cgctgctgtt cgcggccatc
     3721 gctccggcgc aagccggcga ggcggcggcg ccgggcccgt gcctggggcc ggcggtcggc
     3781 gtcgcggtgc cgctgtgccg gcagatcgcc gcgcaccagc ccaacggccc gtggatcccc
     3841 tcgggccggc ggctcaccta tgcctccggc cggccgcgcg agggcgaggt gcgccgcctc
     3901 gaccggttcg ggctcgaggt cgcggagatc accggcgcgc tcgccgcgcg ctaccggatc
     3961 acccaatcgg tcgccggcgt ggtggtggtc ggcatcgcgc cggccggccc ggctgccggc
     4021 aaggggcttg cggttggcga cgtgatcgtc gagctcgacc agcagccggt ggcggggccc
     4081 ggcgacctgt cacggcggct cgaggcggtc gggcgcgcgc ccgcgacctc ggcgctgctg
     4141 ctgctctatc gcgactacga gcaccgcttc caggtgctcg ctctcgactg acgcgagcgg
     4201 cggctacagc agccccagag tcttgaacga ggcgtggccc ttgcgcccga tcaccaggtg
     4261 gtcgtggacc gcgatgccga gcgccttcgc cgcctcgacc acctggcggg tggtgtcgat
     4321 gtcctggcgc gagggcgtcg ggtcgccgct tgggtggttg tggaccagga tcagcgcgct
     4381 ggcgccgagg tcgagcgcgc ggcgcaccac ctcgcgcgga tagaccggcg cgtggtcgac
     4441 cgtgccgcgc tgctgcagct cgtcggcgat cagcacgttc ttgcggtcga ggaacagcac
     4501 ccggaactgc tcggttgcgt tgcgctgcat cgccgccgcg cagtagtcga gcagcgccgc
     4561 ccacgaggcg atcaccggcc ggtcaaggat tcggtcgcgc aacatgcggt gggctgacgc
     4621 ctcaatcacc ttgagcgtcg ccaccaccgc ttcgtcgacc ccgcgcacgt cgcgcagccg
     4681 cgccggctcg gcgccgatca cctcggcgaa cgtgccgaag cgggcgagca acgacttggc
     4741 caggggcttc acgtcgcgcc gcggcagggc cgcgaacagg gcgagctcca gaagctcgta
     4801 gtcggccatc gactcgccgc cctgggcgag gaaacgcttg cgcaggcgct ggcggtggcc
     4861 ggcggcggtg gcacgcggga ccacccgcat gcgctcctcc gcgtcgttcc cggccatgcc
     4921 cccagccgcc gcgcgacggt caggccgtgt agggcggccg gtcgaggccc gccggcgagg
     4981 cggtgaacac ctcgtggccg tccgccgtga ccgcgaccgt gtgctcgaac tgcgccgaca
     5041 gcgagcggtc cttggtcacc gcggtccagc catcggccag gatcttggtc tcgtagcggc
     5101 ccaggttgat catcggctct atggtgaaga acatgccctc gcgcaacgcc agcccggcgc
     5161 ccggccggcc gtagtggagc acgctcggcg catcgtggaa cacctggccg atgccgtggc
     5221 cgcagaagtc gcgcaccacc gagcagtgat ggttctcggc gtaggcctgg atggcgtggc
     5281 cgatgtcgcc cagcgtggcg ccgggacgca ccgccctgat cccgcaccac atcgcctcga
     5341 aggtgacgtc ggtcagccgc gcggccttgc gcggcacgct gtccagccag aacatgcggc
     5401 tggtatcgcc gtgccagccg tcgaggatga cggtgatgtc gatgttgacg atgtcgcccg
     5461 gctgcagccg cttgcgctcg tcggggatgc cgtggcaaac gacgtggttg accgaggtgc
     5521 agatcgagcg cgggaagccg cgatagttga gcggcgcggg caccgcgccg tggtcgacga
     5581 tgaagtcgtg gcacagccgg tcgagctcgc ccgtggtcac ccccgccacc acctgggggg
     5641 cgatgaagtc gagcacctcg gcggccagcc gcccggcact gcgcatgccc tcgaacgcct
     5701 ccgggccgtg aatcttcacc gatcggccgg ggccggacgt ccagtcgttg tcatccacgt
     5761 ctcgcatctc ctggtgcggc gccgcaatgc cgcgcccgcc ccgcctcgcg cgcctgggcg
     5821 gccatccctt atgtagtccg atccgggcgg catttttata gggcgaatcg aggccgccgc
     5881 gccaccgccg cggtcggggc acgatcggcg gcctgcgggg gcgagggcga tctcctcggc
     5941 ggtcgcccgg caggcgtggc acagcgccac gaccccgcgc gcgcgcgccg ggtcgcgccc
     6001 accggcgggt cggggcagcg cacggcatcg ccatcgaacg gttgccgcgg cgtcggcgca
     6061 accgcggtct cgggtcttgg gcggcgccgt tggctcggct attctccgcc cccgggaggc
     6121 ccaggcccat gaaccaagcc gcgtcgtcgc aaccgaccgc ctgcgtcctg atcatcggca
     6181 acgagatcct gtccgggcgc acccaggacg taaacctcaa ctatatcgcc aagcggcttg
     6241 ccgaatgcgg cgtccgcctc gccgaggcgc gggtgatacc cgaccaggag gcggcgatca
     6301 tcgacgcggt caatgcctgt cgcgcggcct atgactgggt gttcaccacc ggcggcatcg
     6361 gcccgaccca cgacgacatc accgccgaat gcatcgccaa ggcgttcggg gtggggctcg
     6421 agcagaacgc cgaggcgcgc gcgctgctcg agcgccagta ccgcgagcgc aatgtcgagc
     6481 tcaacgaggc gcgcctgcgc atggccaaca tgcccgcggg cgcgctgctg gtcgacaacc
     6541 cggtctcggg cgcacccggg ttccaggtcg gcaacgtgtt cgtgctcgcc ggcgtgccct
     6601 cgatcatgca ggcgatgttc gagagcgcgg tcgagcacat cccgggcggg gtgccgctca
     6661 gctcgcgcac cgtggtcgcc tatctgcccg agggcacgct cgccgggccg ctcaaggagc
     6721 tgcagggcga gtacccggac atcgacatgg gcagctaccc gttctaccgg gtcggccggt
     6781 tcggctcgaa cctggtgctg cgcgcggccg acgagggccg gctcgaggag gcggtcaaca
     6841 agctggtcgc gtgcgtcgac cggctcggcg gcgagcccca gctcgagggg tgagcgcggc
     6901 tcagccgcaa caggcgccgc cgccggctcg ccgcgccggc cgccggtgtc aaattggccg
     6961 cggacggcac cccgccgccc tgatcgaggt caaggcttgg atacgcgccc ccggctacca
     7021 agatagggta atttcgggtt cggctcggca gggaggtcga ggatgcaggt gccgatcgag
     7081 gtttcgttca agaacatgga ctcgtcggag gcgatcgcgg ccgacatccg ccagcgcgtc
     7141 gccaagctcg accatttctt cgagcacgtc accggctgcc acgtcagcgt cgaggccccg
     7201 cacaagcacc accgcaaggg acgccattgg cgcgtgcgca tcgtcatcaa cgcccccggc
     7261 cgccagctgg tggtgagcaa cgagcccggc gacctcaacg cccatgagca gctcgcggtg
     7321 gcgctgcgcg atgcgttcaa cgccgccacg cgccagctcc aggaccacgc gcgcaccatg
     7381 cgcggcgacg tcaagagcca ccagccggca caggtcgagg gcagcatcgc gcgcgtcttc
     7441 gccgaccagg gctacggctt catcgacgca ggccccctcg gcgaggtgta cttccaccgc
     7501 cacagcgtgg tcggcgaccg cttcgccgag ctcgccgcag gcagccgcgt ccggttcgtg
     7561 gtcgccgagg gcgagagcga gcacggcgcc caggcctcga ccgtccaccc gctcgactga
     7621 gaggctgtgg agcaatcgcc gggcgaactc ttcctcgtca tccctgcgca agagcttgtg
     7681 acggaatcgc tgaaccgcca ccgatgatca ggtgcgcgta cagaaatgcc gcgcggggcg
     7741 agcgggggga ggcggcgccg aggtctcggc atgtgtgccg cggcgggggc ttgtgcgagc
     7801 ccgtgctgtc acgcgctggc cattctgagg gcgtgtccgc gtctgaggtt gtgggcgatg
     7861 gagtgcaaca gcgcctcgca gcgggccttg gtgagcccgc gcaagaagaa gcgacgcagg
     7921 ccgtgataat tcaccggcac gcccccgcgc agccagcggt acgccacatg cgcgcgggac
     7981 agccgctcca gctgccgcgc cgagccgacc ccctcgagcg cggcgtgggg ccacaccgcc
     8041 ggcagcacct tcgggtccgg cgtcggccgg cccgcagcct cgtcgcgcgc cttgatccga
     8101 tcgtacagcg gtgcaagctc gagcctctcg acaaacgccc aaaccacgcg cgccggatgg
     8161 tccggcgcaa tctggctgca cagatcgacc atgtcccagt agagctgctg acgatccgcc
     8221 cgcatcaccc gcggtccaga cctcattccg gcctccgtgc catgtccggg accaacagaa
     8281 tcacgattct tcacctgcgg gaatcccgac atcacggatt tcctcacagc ctctgttacc
     8341 ggaatgacag gtgtatgaag aaattcattg ccttgttgcc tgattcttaa cctctttgtg
     8401 atctctgtgt cgttggcggt tcaatcttca agcaataacg acgcgcgctg agcgtcagtc
     8461 atccgatcgg gtgcgctcca ggacctcgtc cgggtcccag acgcggccgt ccttgatcac
     8521 ccggtggatg cgggcgaggt tggccgtgtc ggcgagcggg tcggcgtcga gcgccaccat
     8581 gtcggcgcgc cggccgggcg cgatcaggcc aagttcctcg ccccggccca tcgcgcgcgc
     8641 gccggcgagc gtcgcgctgc gcaggatctc ggcgggactg agccccgcgc gggccatcag
     8701 gcgcagctcc ttgtggagtg cggcgccgtg gacggtgccg atgttgccgg aatcggagcc
     8761 ggcgacgacg gtgacgccgg cggcgtggac gcgcgcaagg ttgtcgatca tcacctggtt
     8821 gaccggcggc accggctggt cgaagtcgag ccacgccggc agcgtctcgc gcggcagggc
     8881 ggcaaggtcg tcgaggctgt cgagcacccg gcgcgcgccg taacgccggt cgaggtcggt
     8941 cagcgcgagc ttctggcgca gcacccgcca gtagccgtca tagacgccga gggtggggac
     9001 gtagggcacg tcgcgggcgc gcatcgccgc gaggaaggcg tcgtcgaccg cggcgtcgtc
     9061 gaccgagtgg accagcaggt cggcgccggc ggcaagcgcc gccttggcgg tggcgagttc
     9121 ggtcgcgtgc acggcggcgc cgaggccgag cgcgtgcgcc tcgtcgatcg ccgcgatcac
     9181 ccagcgccgg gtggtggcga agtcctggtc gccgagcgcc tggatgaacc agaccttgat
     9241 caggtcggcg ccgagcccgg cctggcggcg gacctcggcg cgcgcctgct cgggcgtctc
     9301 gaccagcacc accgcgggct ccgcggcggc gagcgcggca ggcgaccagc tgcccaagag
     9361 ctggccggcc caggcgatgc gcggtgccgg catcgccgcg gcttccgcgc gggcgcgcag
     9421 cggcgcgacc cacgccggcc cgccgaggtc gaggatgccg gtcacgccgg cggcgaagat
     9481 gcgcgccagt gtctggtcga ggtcgctgcg gacccgcgcg atctccgcct cgtaaggaac
     9541 caccgcgcgc aggtcgatca cgtcggggcg cgcgtacagg ccgcccgact ggaacaggtg
     9601 gacgtggccg tcgatcagcc ccgggatcag ccacttgccg gcgagcgcga ccacgcgcgc
     9661 cgagcctggg accgcggtgt cggcgccgac cgcggtgatc agcccgtcct cgaccaccac
     9721 cgtcgcgccc ggcagcggcg cgccgcgggc gacgtcgatc acggtcgcgc cggtgaacgc
     9781 gaccgtctcc gccgccgccg gcccggccgc aagcgcgcac gcgacggcga ggccaatcgc
     9841 ccgccattgg cgacggcatc gggacgcggt ggtggttggt aaggcaacga cacgggcgca
     9901 catgacgttt ctcccaattg gcgatggtgc ggactgcgtt actgcccggg ccggcgcgcg
     9961 ttcgcatcct catacgtgcc ggcacccgtc cggctccgaa ttccttgtga ctcggagctg
    10021 acggtgtgac atgctttgcc aattctatgc cggcttgggc cgagattgtg acgaggcctg
    10081 agttcgcgca ctccgacggg ggggggcctg ggatcggggt tctgaccaac cgcacatagg
    10141 cgttcgcggc cgtgcttcac gccgcgcgca ttacccttgg gcgaaaaggg tccgaagatg
    10201 ctcttgcgta gggaggacgc gcgggcaaaa cggcttgcgg ttacggcacc gcggaaaggg
    10261 ggggagcccg gctcggcgcc gagcatgcgg ggtccagcgc cacatccgct gccgagcgca
    10321 agaagcatgc ccttccatcc gggtatcgtc tcgggcctgg cgacgttggc cgacggggca
    10381 tcggtgttcg ggaccggcct ggtcgtctat atgctgtatg tcggctggca aggccattcg
    10441 cttcccctct atctatcctt tctcactctc gacacgctgt tcgtgctcgc ggcattcggg
    10501 ttggccgggc tctatcactt cgagtccctg agtcatccgg agcgccagct gcacaagatc
    10561 ttcatgattt gcggcttgtc gttcatgatc atgacggtcc ttgccttcgc cttgaagatc
    10621 tccgaagagt tctcgcgcgt ttgggcgttc gcctggttct tcgcggcgac ggcgggcatc
    10681 tgtctcgttc gagttttcgg ccatgcgcag ctcagcgagt gggccgtgaa agggcggctg
    10741 acgcgcaacg tggcggtggt aggggccggc atccagggac agaggctcgt ggaggcgctg
    10801 caggcgagcg aggatccatg gctcaacgtg atcggggtct ttgacgaccg cacgacgcgt
    10861 gttccctcgg aggtgggaaa ggtacccgta attggcggcc tcgggcaact tgtcgacttc
    10921 gtgcgccaga accgcatcga cgacatcgtg atcgcgctgc cgtggtcggc cgagagccgc
    10981 cagcttggga tcgtcgagaa attaagcctc ctgcccgcgc acgtgcagct tgcgccggac
    11041 atgatcgggt tccacttccc ggtctcgcgc tatgatcggc tcggcgcggt ctcctgcctc
    11101 aaggtatcgg caaggccgct cgacggttgg agcgcggtgc tgaaggcggt cgaggattgg
    11161 ctggtcgcaa gcgttatcct gttcgcggtc ctgccgctca tgctcatgat tgcagcggca
    11221 atcaagcttg gcagccgtgg ccccgtcttc tacaggcaag agcgctacgg cttcaataat
    11281 cagctgatcg aggttttcaa gttccgcacc atgtacgacg accccgaccc cgacccggag
    11341 gtcctgcagg cgaggcgcaa tgatcctcgc gtcacgcgcg tgggtgcttt cctcaggcgt
    11401 accagcctgg acgagctacc ccagctgttg aacgtgctga ggggcgagat gtcggtcgtg
    11461 ggtccgagac cgcacgccgt ggcgcacgac gagaagtacg gccgggtgat cgaccggtac
    11521 ctgggtcgat ttcgggtcag gcccgggatc accggctggg cgcaggtcaa tggctggcgc
    11581 ggcgagacgg ataccttggc gaagatgcgg gcgcggatcg agcacgacct gtcctatatc
    11641 gagaactggt cggtcgccct ggacctgcga atactcctgt ggacggtggg aatcgtcgtg
    11701 cggggcagga acgcctactg actgggtttg gcccgtcagg ctcgatcctc gctcggcgag
    11761 ccgacgtttc aaggcactga ccgccgccgt acgggttgcg aaaagagttc actgttgtgc
    11821 tcgcgcgtgc gttgaggaac ggacacccct tgcgagggag cggcgtcgcc ttgacggtgg
    11881 gtgccgccgg gctgctgctc attttggcct cttgggacgg cctggtcgaa atggcgcagt
    11941 catggtcgcg cgaggagtac agccacggct atctgatccc gatcgtggtg gcgtttctgc
    12001 tcgcgcgcgg cctggcgcgc gccgaggccg tgcctgaggg tcgcaggtgg ccgggcccgg
    12061 ccatcgtcgc gctgggtggc gcgatggccg tgctcggtca ggccagcatg atcagagatc
    12121 tttcgatcta cgggttcttt gtggggctgg tcggactcgc ggtgagcgtc cttggtcatc
    12181 gacgcgtggt caaagtgtgg gcgcctgtcg cctacctgtt gttcatggtg ccgcttcccc
    12241 agttcgttta cctgaagctc tcggcggaga tgcaactcct gtcatcggag ctcggcgccg
    12301 ggatggtccg catgctgggc atccccgtgt tcctcgacgg caacgtgatc gatctcggcg
    12361 tttatactct gcaggttgcc gaagcgtgca gcgggctccg gtatctgttt cctctgatga
    12421 gcttcggctt tctgtttgcg atgctgttct ccggcgctcg atggcagaag gtggtcctgt
    12481 tcctttcaag cctcccgatt gcggtgatca tgaacagtgt ccgcatcgcc gtgattgcgg
    12541 tcctcgccaa cgactacggg atcgccgagg ccgaaggctt cttgcacttc ttcgagggct
    12601 gggtgatctt cctcgcctgc gtggcattgc tgttcgccgc ggcctggctg cttgaccgcc
    12661 ttggccggtg ggcgggaaga caaagggcgg ccatcgacct gcgcccgccg gacgcgcgtg
    12721 ctctccgccg gctcgccggc tcgatccgag gcgttagcgc cacattggtc gctagcaccg
    12781 tcctcgcgct ctccgcggga gttgggcaat tggccccgtt cggcgatgcc catgcaaacc
    12841 tcgtacgcga tgacttgagc gcgtttccgg ccgagatcgg gacctggcgc gggcagcccg
    12901 aggcgctgga gcgggaaatc cgtcaagtcc ttgccgcgga tgattacctg gtctcgggct
    12961 tcgtcgcgaa tgactcgggc gcgccggtga gtcttttcat cgcatactat gcgacccaga
    13021 ccgatggccg cgccgcgcat tctccggaaa tctgcatccc gggcggcggc tgggagatcg
    13081 tcagcttgag tagccggagc gtggacctcg agagtgcgag cgaggcgggg ttgcgggtaa
    13141 atcgcgccat catacaaaag ggcaggtacc gtcagctggt gtattactgg ttcgagcaac
    13201 gaggacgacg gctggtgaac gagtacgtgg tgaaatggta catcctgtgg gatgcgctca
    13261 cccgcaagcg gacggacggg gcactcgtgc ggctggtaac gcccttgatc ataggggaag
    13321 acgagccggc ggctgatgcc cgcctcgcca ggttcctgcg cggcgcatat cccgtcttcg
    13381 gcaggttcat tcccgcctga gtacccggag tcggcaagcc tcggaggcgg tcgatcggca
    13441 ttgcggccgg caggcatcgg agccacggtc cgcgcagccc gaacggatcg ctagggcccg
    13501 gcgccctcct tgccgtccgt ccttgcgtcg agctcggcca gaacctggcg cacatcggcg
    13561 gtaaagtacg aagtgccgcc gtcgccaaga gcattcgtga ggtatgcgcg cgcgctttcg
    13621 gtctggttgt cggcaagata ggccatgccg aggtggtagt tgagctccgc gttcgacggg
    13681 ctgagttgca ctgcctgttt caggaacagg atggcctggc tgatcgagcc cagccgatag
    13741 tgcgcccatc ccaaggtgtc gatgagcagc ggattgtcgg aggtctggaa accctcggcc
    13801 aggtccagtg cccgttggag gcgtgtcgtg tcttgattgt cgtggtcggc gatcagcgct
    13861 gcgaggttgt tggcggcaac cgtggaccgt ggcgaactct cgagtatctg ttcgtagagc
    13921 gcgatggcct ccgtgaagcg ttcggcgttt tgcaacgcca tgcccagcgc gagcccgata
    13981 tcctcgacgc cgggattgct cgcgtgcgcc gcctgcagca cgtcgatcgc ctggtcgaga
    14041 gtctcgcgct tggcgtacag ctgggcaagc cggaggtacg cgctcggcgt ggacacggcg
    14101 agctctacgg ctcgccggta ggcctggatt gccgcgtcgc cgtcgcccag ttgcgattcg
    14161 acgtcgccca gaagcaccaa gag
//
LOCUS       DASWHU010000119        10300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_50981, whole genome shotgun sequence.
ACCESSION   DASWHU010000119 DASWHU010000000
VERSION     DASWHU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50981"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGB88_04155"
     CDS             <1..252
                     /locus_tag="VGB88_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9646665.1"
                     /translation="AGWHMSAKLDIPGNITLLPLPPRSPELNPAENVWQFLRDNWLSN
                     RVFTSYDDILDHCCDAWNKLIDQPWRIMSIGLRGWAYGF"
     gene            complement(269..1807)
                     /locus_tag="VGB88_04160"
     CDS             complement(269..1807)
                     /locus_tag="VGB88_04160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX9646666.1"
                     /translation="MIPDPDLLAVARPSVPQVHPGERRHAVVDVGSNSIRLVVFDGLK
                     RAPLPLYNEKVMCGLGRRLDASGRLDPDGVELARANLQRFADLISAMGVDSVDAVATA
                     AVRDAEDGPAFVADVRRESGLELRVLTGPEEAELSALGVLAGIPDADGVVGDLGGGSL
                     ELVQIGDGAPGAQATLPLGPLRLPALGDAAASKLSAPIDRVLAGVPWLGAAGGRTLYA
                     VGGSWRALARIHIAQADYPLRVIHRYRVRAGEFAGLARLVSRQSRESLVQMHGVTRRR
                     LDVLPQAAAVLERLIAALAPAEVAFSAYGLREGLVYGRLSAAVRRQDPLLSACRDVAA
                     RAARFPEHAAELVNWTRKLFADEPAEDTRLRHAACLLSDIAWRTHPDFRAVHAFNEIL
                     YYPALHAEHSGRAFLALAVFARYTSKEPPELLGRIRAMIGEEAAQRARIIGLAARLGE
                     TISGGVPGMIERFALRPAHNRGVLRLRYLRRDERLIGEVVVKRFEALARAMELAPELE
                     LRTD"
     gene            complement(1824..3995)
                     /locus_tag="VGB88_04165"
     CDS             complement(1824..3995)
                     /locus_tag="VGB88_04165"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX9646667.1"
                     /translation="MTEIAQQAEIRDRAAQGAEAAAFGDPRARYLNRELSWLAFNWRV
                     MAEAENKRNPLLEQVRFLSISASNLDEFFMVRVAGLMAQQRNGVTQPSLDGRTPVEQL
                     AEVSADAARLLETQQTVWRRLRRELAEAGIRVVDPGELSADDVAWLGGYFLDQVFPVL
                     TPMAIDPAHPFPFIANRGFALVMHLVAGSDGRHLRALVPVQQQIPRFVRLPGAEVRFI
                     AVEQLVELFIDQLFPGYAISGHGTFRIIRDSDIEYEEEAEDLLLLFENVLRRRRRGTI
                     VRLAVSVDTPSELREMVIDELGARRDDVTVIDGMLDLARLDEIIDPDRHDLLFPPFRP
                     RFPERIREHDNDCLAAIRHKDIIVHHPYESFDVVVQFLEQAATDPNVLAIKQTLYRTT
                     AESPIARALIAAAEAGKSVTALVELKARFDEETNVKLSRSLERAGVHVVYGFMNLKTH
                     AKVTCVVRREGGGLRSYVHFGTGNYHPVTAKVYTDLSFFTCAEPFGRDAQRVFNFTTG
                     YAVPDSFAKIAIAPFSLRKRLIELIDEEIAHARAGRPAGIWAKMNALVDGTIIDKLYQ
                     ASEEGVHIDLVVRGICCLRPGLKGLSSNIRVKSIIGRFLEHSRIVCFGNGRALPSPVA
                     KVFISSADFMPRNFDRRCEVLVPIENPTVHEQVLDQIMVANLKDEAQSWVMQGDGSYT
                     RLPVRDDSFSAHTYFMTNPSLSGRGKALQKGGGPARLQVAS"
     gene            complement(4139..4918)
                     /locus_tag="VGB88_04170"
     CDS             complement(4139..4918)
                     /locus_tag="VGB88_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9646668.1"
                     /translation="MDLGIAGRKAIVCASSKGLGRACAMALAREGVDVTINARTADVL
                     EQTAAEIRSRTGAKVTAVAADVTTADGQARLLAACPAPDILVNNAGGPPPGDFRDWSR
                     EDWLRGIVANMLTPIELIKATVDGMIARRFGRIVNITSSSTKSPIPTLGISNGARTGL
                     AGFVAGLARQTVRHNVTVNCLLPGPFETDRMRGNLRFRAEKAGKDYDGFAKAEAEANP
                     AGRFGDPAEFGDACAFLCSAQMGFVTGQHFLMDGGNYRGTF"
     gene            complement(4918..5622)
                     /locus_tag="VGB88_04175"
     CDS             complement(4918..5622)
                     /locus_tag="VGB88_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX9646669.1"
                     /translation="MTAAPARQLALALGHRPALGAGDFLVAAANRDAVAWIDRWPDWP
                     ATGLVLHGPPGCGKTHLVAVWCARTGTTALPAAALGAVGPDAALGANPALAIEDIDRG
                     VDETALFHLFNMASERGVKLLLSARTPPGRLRVALPDLDSRLCALPAVAISAPDDDLL
                     AALLVKQFADRQVTVAPEVIEFLIARIERSFAAAQRAVDALDRGAMARRRRITVALAR
                     ELLTELTTEQTDTGGT"
     gene            complement(5619..6686)
                     /locus_tag="VGB88_04180"
     CDS             complement(5619..6686)
                     /locus_tag="VGB88_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9646670.1"
                     /translation="MSPARQVLFWTAIAVAFVVVLVVFGDVLLPFVAGMAIAYFLDPV
                     ADRLEAAGLSRTVATTLVLALFFVAFVTVLLLLVPLLQGQLVELVRQLPAVFDSISAG
                     VTGLIERATSSFDEAQVGEAKRALADLKTQLVTWLLNLIGGVWASGVALVNLIGLLVI
                     TPVVAWYLLRDWDALVARIDRWLPRDHAEVIRAQVRLIDRALAGFVRGQATVCVILGA
                     GYALALELAGLRYGLAIGLVAGVISFIPYVGSLTGLLLGLGLGYAQFGLSLHLAAIAA
                     IFFAGQLIEGNYLTPKLVGGRVGLHAVWVIFALLAGGSLLGFVGILLAVPVAAVIGVL
                     TRFALGRYLDSRLYRGTGAGA"
     gene            complement(6686..7231)
                     /locus_tag="VGB88_04185"
     CDS             complement(6686..7231)
                     /locus_tag="VGB88_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9646671.1"
                     /translation="MNAPNAISLARFLCVPLVVWLIVSGRAQAAFAVFVVAGISDAVD
                     GFLAKRYGLTTALGEHLDAVADKSLIVAAYATMGIAGWLPAWLVILVLFRDVMIVGGW
                     LLLTAVAGPRRVRPAWSSKVNTAAQIVLAAAALAGQAFWPIPGWLATTLVGVVAATTV
                     ISGIAYLRRGIAQAGELERLP"
     gene            complement(7238..8458)
                     /locus_tag="VGB88_04190"
     CDS             complement(7238..8458)
                     /locus_tag="VGB88_04190"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX9646672.1"
                     /translation="MTPRQRNGRRLCRWATLGALLWPLAPAVAGDLFSVAGITVDATA
                     ENAELAQQQAIALGQRRALEAMFRKLTLDVDEARLPEATPELLTRLARGYEVVNERRS
                     GQRYIAELAVRFDPEGVREALRQAGIPYAETVSPPLVVLPVAVDAAGARLWAEPNPWL
                     AAWRNLDWRDRLVALVVPFGELADVAAITAEQALAGERAPLAAIAARYQAVASLVAAA
                     QSLPEGGVTVAVGRYGSGEPEILMREHFAPAAAATEEQLYRPMAEAVAMTLERAWKRE
                     NLLDFRVKESLPVSARLSGLDDWVKLRRSLDALSQIERLEVESLGTREARLTLHFFGP
                     RDRLAEALAARQVALSEDAAGRWRLELTAAGRAAAAGPAAVLAPAPDMPVPAAEPAAP
                     APAPRPPSESLLVE"
     gene            8624..9283
                     /gene="purN"
                     /locus_tag="VGB88_04195"
     CDS             8624..9283
                     /gene="purN"
                     /locus_tag="VGB88_04195"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9646673.1"
                     /translation="MSGPLRLAIVISGRGSNMRALVDACRAEAFPAEVGVVISNRADA
                     AGLAAARAAGVPVRVLDEATFEHREAFDAALDEAVADAGASLVCLAGFMRLLGTPFVE
                     RWRDRLINVHPSLLPAFRGLDTHARVLAAGARFSGCTVHFVRPAMDEGPIIVQAAVAV
                     HTGDSPASLARRVLAQEHRIYPLAVRWIADGRVRVVDERVEIDGALSPDMALVNPGPA
                     R"
     gene            complement(9304..9726)
                     /gene="ndk"
                     /locus_tag="VGB88_04200"
     CDS             complement(9304..9726)
                     /gene="ndk"
                     /locus_tag="VGB88_04200"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9646674.1"
                     /translation="MAVERTLSIIKPDATRRNLTGKIIATFEEASLRIVASKRMRLTR
                     DQAERFYAVHQERSFFDGLCTFMCSGPVVVQVLEGENAIERNRQLMGATNPANAAPGT
                     IRREHGESIEANSVHGSDNADNAAWEVAFFFGDVEIVG"
     gene            complement(10049..>10300)
                     /locus_tag="VGB88_04205"
     CDS             complement(10049..>10300)
                     /locus_tag="VGB88_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HEX9646675.1"
                     /translation="MQMRRYTRLTNAHSKKFENHCHMVALYTVWYNYARIHSTVRMAP
                     AMAAGISDRLWSMADIVKLIDDAAPKPGSRGPYKKRIAA"
BASE COUNT         1540 a         3696 c         3661 g         1403 t
ORIGIN      
        1 gccggctggc acatgtccgc caagctcgac atccccggca acatcaccct cttgccgctg
       61 ccgccgcgtt ccccggagct caatccggcc gagaacgtct ggcagttcct gcgcgacaac
      121 tggctgtcca accgggtctt cacctcctac gacgacatcc tcgaccactg ctgcgatgcc
      181 tggaacaagc tcatcgacca accgtggcgg atcatgtcca tcggcctccg aggctgggcc
      241 tatgggttct aatcaatgca tgttggtatc agtcggtgcg cagctcgagt tcgggcgcga
      301 gctccatggc gcgggcgagc gcctcgaagc gcttgaccac gacctcgccg atcaggcgct
      361 cgtcgcggcg caggtagcgc agcctgagca cgccgcggtt gtgcgcgggc ctgagcgcga
      421 accgctcgat catgcccggg acgccgcccg agatggtctc gccgaggcgc gccgcaagcc
      481 cgatgatgcg cgcgcgctgc gcggcctcct cgccgatcat ggcgcgaatc cgcccgagca
      541 gctcgggcgg ctccttgctg gtgtagcgcg cgaacaccgc cagcgccagg aacgcccgcc
      601 ccgaatgctc ggcatgcagc gccgggtagt agaggatctc gttgaacgcg tgcaccgccc
      661 ggaagtccgg atgggtccgc caggcgatgt cgctcaacag gcacgcggca tggcgcagcc
      721 gcgtgtcctc ggcgggctcg tcggcgaaca gcttgcgcgt ccagttgacc agctccgcgg
      781 cgtgctcggg aaagcgcgcc gcgcgggcgg cgacatcgcg gcaggcgctg agcagcgggt
      841 cctggcgcct gaccgccgcc gacaggcggc cgtagaccag gccctcgcgc aggccgtagg
      901 ccgagaacgc cacctcggcc ggcgccagcg ccgcgatcag ccgttcgagc accgccgccg
      961 cctgcggcag cacgtcgagg cggcgccggg tcacgccatg catctgtacc agcgactcgc
     1021 ggctctggcg cgacaccagg cgggcgaggc cggcgaactc gccggcgcgc acccgatagc
     1081 ggtggatgac gcgcaacgga tagtccgcct gggcgatgtg gatgcgggcg agcgcgcgcc
     1141 agctgccgcc gaccgcgtac agggtgcgcc cgcccgccgc cccgagccag ggcaccccgg
     1201 cgagcacccg gtcgatgggc gcggacagct tcgaagcggc agcgtcgccg agcgcgggca
     1261 gccgcagcgg cccgagcggc agggtcgcct gcgcacccgg cgcgccgtcg ccgatttgga
     1321 ccagctcgag actgccgccg ccgaggtcgc cgaccacgcc gtcggcgtcg gggatgccgg
     1381 cgagcacgcc gagcgccgac agctccgcct cctcgggccc ggtcagcacc ctgagctcga
     1441 gcccgctttc gcggcgcacg tccgcgacga aggccgggcc gtcctcggca tcgcgcaccg
     1501 cggcggtggc caccgcatcg acgctgtcca cgcccatcgc cgagatcaga tcggcaaacc
     1561 gctgcaggtt ggcgcgcgcg agctcgaccc cgtcggggtc cagacggccg ctggcgtcga
     1621 gccgccggcc gagcccgcac atcaccttct cgttgtagag cggcagcggc gcgcgcttga
     1681 gcccgtcgaa caccaccagg cggatcgagt tcgagccgac gtcgaccacc gcatggcgcc
     1741 gctcgcccgg atgcacctgc ggcaccgacg gccgcgccac cgcgagtagg tcgggatccg
     1801 gaatcatccg gatgcgaccg gcctcacgag gcgacctgca gccgcgccgg gccgccgccc
     1861 ttctgcaggg ccttgccgcg gcccgacagg ctggggttgg tcatgaaata ggtgtgggcc
     1921 gagaacgagt cgtcgcgcac tggcagcctg gtgtagctgc cgtcgccctg catcacccag
     1981 gactgcgcct cgtccttgag gtttgcgacc atgatctggt cgagcacctg ctcgtgcacg
     2041 gtcgggttct cgatcggcac cagcacctcg cagcgccggt cgaagttcct gggcatgaag
     2101 tcggcggacg agatgaacac cttggcgacg ggcgagggca gcgcgcgccc attgccgaag
     2161 cagacgatgc gcgaatgctc gaggaaccgg ccgatgatgc tcttgacccg gatgttcgac
     2221 gacagcccct tgaggccggg gcggaggcag cagatgccgc gcaccaccag gtcgatatgc
     2281 acgccctcct cgctcgcctg gtacagcttg tcgatgatcg tgccgtcgac cagcgcgttc
     2341 atcttcgccc agatgccggc cgggcgcccg gcgcgggcat gggcgatctc ctcgtctatc
     2401 agctcgatca gccgcttgcg cagcgagaac ggcgcgatcg cgatcttggc aaacgagtcc
     2461 gggaccgcgt agccggtggt gaagttgaac acccgctgcg cgtcgcgccc gaacggctcg
     2521 gcacaggtga agaacgagag atcggtgtag accttggcgg tcaccgggtg atagttgccg
     2581 gtgccgaagt ggacgtagga gcgcaggccc ccgccctcgc ggcgcacgac gcaggtcacc
     2641 ttggcgtggg tcttgaggtt catgaagccg tagaccacgt gcacgccggc ccgctcgagg
     2701 ctccgcgaca gcttgacatt ggtctcctcg tcgaaccgcg ccttcagctc gaccagcgcg
     2761 gtcaccgact tgcccgcctc ggcagcggcg atcagcgccc tcgcgatcgg cgactcggcg
     2821 gtcgtccggt acagcgtctg cttgatcgcc agcacgttgg ggtcggtcgc cgcctgctcg
     2881 aggaactgca ccaccacgtc gaagctctca taggggtggt ggacgatgat atccttgtgc
     2941 cggatggcag ccaggcaatc attgtcgtgc tcgcgaatgc gttccgggaa gcgcggccgg
     3001 aacggcggga acagcaggtc gtgacggtcg ggatcgatga tctcgtcaag ccgcgccaag
     3061 tcgagcatgc cgtcgatcac ggtcacgtcg tcgcgccggg cgcccagctc gtcgatcacc
     3121 atctcgcgca gctcgctcgg cgtgtcgacg ctgaccgcga ggcgcacgat ggtgccgcgc
     3181 cgccgccggc gcagcacgtt ctcgaacaac agcagcaggt cctcggcctc ctcctcgtac
     3241 tcgatgtcgc tgtcgcggat gatgcggaag gtgccgtggc cgctgatcgc gtagccgggg
     3301 aacagctggt cgatgaacag ctcgaccagc tgctcgacgg cgatgaagcg cacctcggcg
     3361 ccgggcaggc ggacgaagcg ggggatctgc tgctgcaccg gcaccagtgc gcgcaggtgg
     3421 cggccgtcgc tgcccgccac caggtgcatg accagcgcga agccgcggtt ggcgatgaac
     3481 gggaacgggt gcgccgggtc gatcgccatc ggggtgagca ccgggaacac ctggtcgagg
     3541 aagtagccgc cgagccaggc gacgtcgtcc gccgacagct cgcccggatc gacgacgcgg
     3601 attccggcct cggcgagctc gcggcgcagc cggcgccaca ccgtctgctg ggtctccagc
     3661 agccgcgccg cgtcggcgct cacctcggcc agctgctcga ccggcgtgcg gccgtcgagg
     3721 ctcggctggg tgacgccgtt gcgctgctgc gccatcaggc cggcaacgcg gaccatgaag
     3781 aactcgtcga gattgctcgc cgagatcgac aggaagcgca cctgctcgag cagcggattg
     3841 cgcttgttct cggcctcggc catgacccgc cagttgaacg ccagccaaga cagctcgcga
     3901 ttgagatagc gcgcgcgagg gtcgccgaac gccgcggcct ccgctccctg tgccgcccga
     3961 tcccggattt cggcctgctg cgcgatctcg gtcatgttcc gctcccgccc gtcctcttca
     4021 atatagcagc cccgggcatg gctttcgagc ccgggccgcc gccgccggcc gtcaccgaac
     4081 ggcaacaaag ggcgggccgg gctgcgccgg ttcgatagcc ggctagctag ccggcgggct
     4141 agaacgtgcc gcggtagttg cccccgtcca tcaggaagtg ctggccggtg acgaagccca
     4201 tctgggccga gcacaggaac gcacaggcgt cgccgaactc ggccggatcg ccgaaccgtc
     4261 cggccgggtt ggcctcggcc tcggccttgg cgaagccgtc gtagtccttg cccgccttct
     4321 cggcgcggaa gcgcaggttg ccgcgcatcc ggtcggtctc gaacggcccc ggcagcaggc
     4381 agttgacggt gacgttgtgg cgcacggtct ggcgggcgag gccggcgacg aagccggcga
     4441 gcccggtgcg cgcgccgttt gagatgccga gggtggggat cgggctcttg gtcgagctcg
     4501 aggtgatgtt gacgatgcgc ccgaagcggc gcgcgatcat gccgtcgacg gttgccttga
     4561 tcagctcgat cggggtcagc atgttggcga cgatgccgcg cagccagtcc tcgcgcgacc
     4621 agtcgcggaa gtcgccgggc ggcgggccgc cggcgttgtt gaccaggatg tctggcgctg
     4681 ggcaggcggc gagcagccgc gcctggccgt ccgccgtggt cacgtcggcg gcgaccgcgg
     4741 tgaccttggc gccggtccgg ctgcggatct ccgcggcggt ctgctcgagc acgtccgcgg
     4801 tgcgcgcgtt gatcgtcacg tcgacgccct cgcgcgcgag cgccatcgcg caggcgcgcc
     4861 ccaggccctt gctcgatgcg caaacgatgg ccttgcggcc ggcgatgccg agatccatca
     4921 cgtccctccg gtgtcggtct gttccgtcgt cagctcggtc aacaactcgc gcgccagcgc
     4981 cacggtgatc cggcgccggc gcgccatcgc gccgcggtcg agcgcgtcca ccgcgcgctg
     5041 ggcggcggcg aacgagcgct cgatgcgcgc gatcaggaac tcgatcacct cgggcgcgac
     5101 cgtgacctgg cggtccgcga actgcttgac cagcagcgcc gccagcaggt cgtcgtcggg
     5161 cgcggagatc gctaccgccg gcaacgcgca caggcgcgag tcgaggtccg gcagcgccac
     5221 ccgcaggcgc ccgggcgggg tgcgcgcgct gagcagcagc ttgacgccgc gctcgcttgc
     5281 catgttgaac aggtggaaca gcgcggtctc gtcgacgccg cggtcgatgt cctcgatcgc
     5341 gagcgccggg ttggcgccga gcgccgcgtc ggggccgacg gcgccgagcg cggccgccgg
     5401 cagcgccgtt gtgcccgtgc gcgcgcacca caccgccacc aggtgggtct tgccgcagcc
     5461 gggcgggccg tgcagcacca gcccggtcgc cggccagtcg ggccagcggt cgatccaggc
     5521 gaccgcgtcg cggttggccg ccgccaccag gaagtcgccg gcgccgagcg ccgggcggtg
     5581 gccgagcgcg agcgcgagct ggcgtgcggg cgccgcggtc acgctcccgc ccccgtgccc
     5641 cggtagagcc ggctgtcgag gtagcggccg agcgcgaacc gggtcagcac gccgatcacc
     5701 gccgccaccg gcaccgccag caggatgccg acgaagccga gcaggctgcc gccggcgagc
     5761 agggcgaaga tcacccacac cgcgtgcagc ccgacccggc cgccgaccag cttcggggtc
     5821 aggtaattgc cctcgatcag ctggccggcg aagaagatcg cggcgatcgc cgccagatgc
     5881 aggctcagcc cgaactgggc atagccgagg ccgaggccga gcagcaggcc ggtgagcgag
     5941 ccgacatagg ggatgaacga gatcaccccg gcgaccagcc cgatcgccag cccgtaacgc
     6001 agcccggcga gctcgagcgc cagcgcgtag ccggcgccga ggatcacgca cacggtcgcc
     6061 tggccgcgca cgaagccggc cagcgcgcgg tcgatcagcc gcacctgcgc gcggatcacc
     6121 tcggcatggt cgcgcggcag ccaccggtcg atgcgcgcca ccagcgcatc ccagtcgcgc
     6181 agcaggtacc acgccaccac cggcgtaatc accagcagcc cgatcaggtt gaccagcgcg
     6241 actcccgacg cccacacccc gccgatcagg ttgagcagcc aggtcacgag ctgggtcttc
     6301 aggtcggcaa gggcgcgctt cgcttcgccg acctgggcct cgtcgaagct gctggtcgcg
     6361 cgctcgatca gcccggtgac gccggcgctg atcgaatcga acacggcggg aagctggcgc
     6421 accagctcga ccagctggcc ctgcaggagc ggcaccagca acagcaggac ggtgacgaag
     6481 gcgacgaaga acagcgccag cacgagcgtg gtggcgacgg tgcgcgacag gcccgcggcc
     6541 tcgagacggt cggcgaccgg atcgaggaag taggcgatcg ccatgccggc gacgaacggc
     6601 agcagcacgt cgccgaacac caccagcacg accacgaagg cgaccgcgat cgccgtccag
     6661 aacagcactt ggcgggcggg gctcatcacg gcaggcgttc gagctcgccg gcctgggcga
     6721 tgccgcggcg cagataggcg ataccggaga tcaccgtggt tgcggcgacc acgccgacca
     6781 gcgtcgttgc cagccagccg gggatcggcc agaacgcctg gccggcgagc gccgcagcgg
     6841 cgagcacgat ctgggccgcg gtgttgacct tgctcgacca cgccgggcga acccgcctcg
     6901 gcccggcgac cgcggtcagc agcagccagc cgccgacgat catgacgtcg cggaacagca
     6961 ccaggatcac caaccacgcc ggcagccaac cggcgatgcc catggtcgcg taggcggcga
     7021 cgatcaggga cttgtcggcg accgcgtcga ggtgctcgcc gagcgccgtg gtcagcccgt
     7081 agcgcttggc caggaagccg tccaccgcat ccgagatgcc ggcgacgacg aacaccgcga
     7141 acgccgcctg ggcgcggccg ctgacgatca gccacaccac cagcggcacg cacaggaacc
     7201 gggccaggct gatggcgttc ggggcattca cgccgcgcta ctcgaccagc agcgactcgc
     7261 tcggcgggcg cggcgccggc gcgggcgcgg ccggctcggc cgccggcacc ggcatgtccg
     7321 gtgcgggcgc gagcaccgcg gccgggcccg ccgccgcggc tcggcccgcc gccgtcagct
     7381 ccagccgcca gcggccggcc gcatcctcgc tgagcgcgac ctgtcgcgcc gccagcgcct
     7441 cggccaggcg gtcgcggggg ccgaagaagt gaagggtcag ccgcgcctcg cgggtgccca
     7501 gcgactcgac ctcgaggcgc tcgatctggg acagggcgtc caggctgcgg cgcagcttga
     7561 cccagtcgtc gaggccggac aggcgcgcgc tgaccggcag cgattccttg acccggaagt
     7621 cgagcaggtt ctcgcgcttc caggcccgct cgagggtcat cgccacggcc tcggccattg
     7681 gccggtagag ctgctcctcg gtcgccgctg cggccggcgc gaagtgctcg cgcatcagga
     7741 tctcgggctc gccgctgccg tagcggccga ccgcgaccgt gaccccgccc tcgggcaggc
     7801 tctgcgccgc cgccaccagg ctggccacgg cctggtagcg cgccgcgatc gcggccagcg
     7861 gcgcgcgctc gccggccagc gcctgctcgg cggtgatcgc cgccacgtcg gcaagctcgc
     7921 cgaacggcac caccagcgcg accagccggt cgcgccagtc gaggttgcgc caggcggcga
     7981 gccatgggtt cggctctgcc cacaggcgcg cgccggcggc atcgaccgcc accggcagca
     8041 ccaccagcgg cgggctcacg gtctcggcgt aggggatgcc ggcctggcgc aaagcctcgc
     8101 gcacgccctc ggggtcgaag cggaccgcga gctcggcgat gtagcgctgg ccagagcggc
     8161 gctcgttgac cacctcgtag ccgcgcgcga gccgggtcag cagctcgggc gtcgcctcgg
     8221 gcaggcgcgc ctcgtcgaca tcgagggtga gcttgcggaa catcgcctcg agcgcccggc
     8281 gctggccgag cgcgatcgcc tgctgctggg cgagctcggc gttctcggcg gtggcatcga
     8341 ccgtgatccc ggcgaccgag aacaggtcgc cggcgacagc cggcgcgagc ggccagagca
     8401 gcgcgccgag ggtcgcccag cggcacagtc gccgcccgtt gcgttggcgg ggagtcattg
     8461 caaaccgccg tcgatcccgt aaggtctgca gccccgcgtc cgccgcaccc ggacgcaggc
     8521 gacatggcgc agcatgaccg catcatacca tgagcgataa ggccggccac ccccatcgcg
     8581 cccgcgccgc gggggtcgat ctcgacaccg gcgccgggcg gcggtgagcg ggccgctcag
     8641 gctcgcgatc gtgatctccg ggcgcggcag caacatgcgc gccctggtcg atgcgtgccg
     8701 cgccgaggcg ttcccggccg aggtcggggt ggtgatctcg aaccgcgccg acgccgccgg
     8761 gctcgcggcc gcccgcgccg cgggcgtgcc ggtgcgcgtc ctcgacgagg cgacgttcga
     8821 gcaccgcgag gcattcgatg ccgcgctcga cgaggcggtc gcggacgcgg gcgcgagcct
     8881 ggtgtgcctg gccggattca tgcgcctgct cggcacgccg ttcgtcgagc gctggcgcga
     8941 ccgcctgatc aacgtccacc cctctctgtt gcccgccttc cgcgggctcg atacccacgc
     9001 ccgcgtgctc gccgccggcg cccgcttcag cggttgcacg gtccacttcg tgcgcccggc
     9061 gatggacgag ggcccgatca tcgtccaggc ggcggtggcg gtgcataccg gggattcgcc
     9121 cgccagcctg gctcggcgcg tgctcgccca ggaacacagg atctacccgc tggcggtgcg
     9181 ctggatcgcc gacgggcggg tgcgcgtggt cgacgagcgg gtcgagatcg acggcgcctt
     9241 gagccccgat atggcgctgg tcaatccggg cccggcgcgc tgagccgccg gccgcgccgc
     9301 gcctcagccg acgatctcca cgtcgccgaa gaagaacgcg acctcccagg ccgcgttgtc
     9361 ggcgttgtcg gagccgtgca ccgagttggc ctcgatcgac tcgccgtgct cgcggcggat
     9421 ggttcccggc gccgcattgg ccgggttggt ggcgcccatc agctggcgat tgcgctcgat
     9481 cgcgttctcg ccctcgagca cctgcaccac caccgggccc gagcacatga aggtgcacag
     9541 gccgtcgaag aacgagcgct cctggtgcac ggcgtagaag cgctcggcct ggtcgcgggt
     9601 caggcgcatg cgcttgctcg cgacgatgcg caggctcgcc tcctcgaagg tagcgatgat
     9661 ctttccggtc aggttgcgtc gcgtggcgtc gggcttgatg atcgagagcg ttcgttcgac
     9721 cgccatacag gttccccttc gcggtgacgg agccggggcg ttatacagat gcccgcccgg
     9781 gccagcaacc gcgctctgac gggggtagtg cctcagtttg aaatcggtgg ggatcagcgc
     9841 gagcggccgg cccaattgcg tagaccagaa acgagcgcaa acagcgcggc accgcctatg
     9901 ccagcgccgg taaggtgccc ggcgatgggc acccaattat caattatggg atcggtgtat
     9961 atctggaaag caccataaat caggccacag agggcgccaa tcagggcaat tctcggcgtg
    10021 ttccacggct ttggcttcat cggtgccgtc aggctgcaat gcgcttcttg tacggcccgc
    10081 gcgagccggg cttgggcgcg gcatcgtcga tcagcttcac gatatcggcc atgctccaca
    10141 gccgatccga gatacccgca gccatcgccg gggccatccg cacggtgcta tggatacggg
    10201 catagttgta ccagaccgta tagagcgcga ccatgtggca gtggttttcg aatttcttgc
    10261 tgtgggcgtt cgtgagccgc gtatagcgcc tcatctgcat
//
LOCUS       DASWHU010000120        21019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2276110, whole genome shotgun sequence.
ACCESSION   DASWHU010000120 DASWHU010000000
VERSION     DASWHU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 21019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21019
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276110"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VGB88_04210"
     CDS             <1..1117
                     /locus_tag="VGB88_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9646676.1"
                     /translation="QRVDAAMAQKAAAHKLAELSWPGYRKFEIARKAAEADAIASFYL
                     VPHNRTPLPPYKPGQYLTFRFQIPGRAKPLIRCYSLSDAPDGDQYRITVKRLPPPHEP
                     AGLPPGLASSFLHDAVSHGHLLDVMAPTGHFFLDEASERPVVLIGGGIGLTPVLAMLN
                     AIARSGRTRESWLFYAVHNRRQEVMGEHLREIASGNPNIHVRIAHSRPDQDEQAGRDY
                     DYPGHLTIDVLKRELPSNNFEFYVCGPPPMMSKMIADLRAWQVPEERIHFEAFGAATI
                     QQIGAAKDGAQSFEVVFERSGKTLTWRAEAGTILDLAEKNGVAIDYGCRAGNCGTCVT
                     AVRAGEITYLGEPGASAESGTCLTCISIPKTRLVLDA"
     gene            1239..2267
                     /locus_tag="VGB88_04215"
     CDS             1239..2267
                     /locus_tag="VGB88_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9646677.1"
                     /translation="MASIMMGKLVGWTHRGALAGIALMALAATGWAGPDPAKVIGPDE
                     CGECHKAEVNVWRKTHHYTTFQELPRKDKAREIADKMDVRRLKSESLCLKCHFTEMTK
                     DDGSTDTIAGIGCESCHGGAKDWNKIHSDYGGKDVKRDSEAPEHRTQRIAASRAAGMI
                     QPSMLYDWANNCYSCHTVPEEKLVNVGGHTAGSKFELVTWTQGEIRHNVWYTDAKSNP
                     EASQEQKRLMLVVGQMLDLEHALRGVAKATERADYAVAMAGRAKAAAQRIKQISEAQT
                     TPEIDAVLAVAGSVELKLGNADQLTAAADKIGEQARALAANNDGSALAGLDGLIPGAD
                     QYKGTPSQ"
     gene            2379..4103
                     /locus_tag="VGB88_04220"
     CDS             2379..4103
                     /locus_tag="VGB88_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9646678.1"
                     /translation="MARPRVELHRPERWDNPFSPEMTAEQVERLLAMPPFDEIDASKF
                     PPTNSLADIVRNDTRIRDFAPGDIVVREADYGNSAFVILDGRVRVIQAPPLPAQALGR
                     QPPRRRSPWQALAQLWTNPRTAERRDAARYRGDAQVRLTRAGEDEFRVTLADPDGVIA
                     RHRTAQMGPGEMFGEIAAVARGARAATVFAETRATLLEIRWQGLRDMRRRDARFRDKV
                     DELYRKRALGAHMRESPLFKGLDDEPMKEVSRTTQFASFGEFDWHTSYKALAGKTAPE
                     RLEDEPIIVTEDQVADALIMVRSGFARVSERVDHGHRTVNYLGPGDVFGLAEIVYNWR
                     NDAHITYQHTLRATGSVEVLRVPAQVIREHVLPGLPRALLPPPIIPRSGARSAWQADR
                     VAEDIPPAMLEFLVDNRLINGTAAMVIDVDRCTRCDECVKACAATHDNNPRFVRHGLL
                     FDHFMVANACMHCVDPVCMIGCPTGAIHRNVQAGQVVINDDTCIGCGSCANACPYNNI
                     RLVDIRDDDGNFMLDEATNMPLEKATKCDLCADQIGGPACQRACPNDALVRINLRDLD
                     RLAEWVGR"
     gene            4100..4912
                     /locus_tag="VGB88_04225"
     CDS             4100..4912
                     /locus_tag="VGB88_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646679.1"
                     /translation="MRYFAVRLRRNLALTGAACLALLMVYAVHGASLAHENFVTGWLL
                     VAAIAVLVLYNARRGAPALPLGSSTAWMQLHVYLAIVAIFLFLLHVGIEVPRGIMEKV
                     LAGLFVAVAGSGVFGFVLARLLPARLSRRGEVMLYERIPQFRRQLLERGEALALRAVE
                     ETGSTTIADYYADRLRPFLDGPRNFWAHVLEVSRGRRDLLVEMRDLERYLDDNEKEIL
                     RELAELSGLKDDLDYQYALQSMLKRWQFVHVPLAYALIVFVLLHAVVVYAFA"
     gene            4914..7244
                     /locus_tag="VGB88_04230"
     CDS             4914..7244
                     /locus_tag="VGB88_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646680.1"
                     /translation="MPTSKRLLNFGHRESPYDRPNQSWVCGHLVDGRACAIGPDKRGR
                     CRATAECLPIRKDDRWQCARPAQHGGRCTEGPHPDGGCGRPIATCQPVRSLAARRAVA
                     VRWTLIASLGLVLLVGSGVSGREFVFSGELTFQHGEVGRCTGCHSVPDHGPISWLAAA
                     FRDRASIDDSGLCLKCHRLGEDALAAHDLPRATVSAITERQSAKAAQAPLLLKLAKLT
                     IGAPAGDQPVACGTCHREHNGRRFDLTAMSDLRCQTCHRGQFASLADGHPRFEGFPYR
                     RRTRIAFDHASHIGKHFKADSAIDAPTSCTGCHVLEPAGRAMEVKPFEVSCAACHLGQ
                     IMGEGRAGTKGYPVLILPGFDIETLYARGHEVGDWPRDADGKLTSFMNLLLANDPAHA
                     EDLFVLSGVDLMDLREATEEQLDAAARTIWSIKDLIYDLAVTGHEALRARVAGALSVE
                     VPRYESGPLMGTMPVDAVASAQQAWFPRLAGDIERHRAGEPVPPPDWIASPAGEASTP
                     VAPADAGAPAPADQDLGGDLLGGDEGGLLGGDEGGLLGGGEGGLLSEPAPEPGAGQEQ
                     VEAAEPAAVPESMMPEDIEGWMRAGGWYRQGYALLYRPIGHRDPFMRAWIDLTAGVGP
                     SPGAPHALQIFAEITSRKAPGMCGKCHSVDATADGTTVVNWRPKQRPQDVNRFTEYAH
                     APHFSLLDEKGCLTCHVIDYEADFMAGFNDLDASTFASNFKPMNKELCASCHVDSLAG
                     DACTDCHNYHIGRFPPALAAAPLAVDAGAGAPAPHD"
     gene            complement(7241..7552)
                     /locus_tag="VGB88_04235"
     CDS             complement(7241..7552)
                     /locus_tag="VGB88_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646681.1"
                     /translation="MARTRPIEQGRGRRAHVARPAVVVLAVALALAGGTAPAMPVDTK
                     ELGDVPGVTGDGELDIREVLDTSTEMDIRLTKQIMLLQRQINDLKKQIETMQAAQAPG
                     N"
     gene            7737..8858
                     /gene="corA"
                     /locus_tag="VGB88_04240"
     CDS             7737..8858
                     /gene="corA"
                     /locus_tag="VGB88_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX9646682.1"
                     /translation="MSAAKSGSQPRRRKRRAIRLRRRSLPGAAPGVLVADPEARESEV
                     NVILYGPDGLEEVRGCPPSELRGLRARAKVLWIDVTGLADLAVIAEIGAAFAIHGLAL
                     EDVVNVHQRPKAEQFEDHVFLVARMLAAEGRAETEQVSVFLGEDYLISFQERRGDCFE
                     PIRERIRRGKGRIREMGADYLAYALIDAVIDSFYPVLEGYGEDIERLEDDVIAGPSPD
                     QVDRVHTVKRDLLTLRRAIWPTREMVNGLIRDESPFVGATTRVYLRDCYDHAIQLMDI
                     VETYREIATGLLDVYLSSMTARLNEIMKVLTIIATIFIPLSFVTGIYGMNFDRAASPW
                     NMPELGWRLGYPMALAIMVAIAGGLLFYFWRRGWLAKRR"
     gene            complement(8872..11619)
                     /locus_tag="VGB88_04245"
     CDS             complement(8872..11619)
                     /locus_tag="VGB88_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX9646683.1"
                     /translation="MDSWAVLAILIAVFALLVVPGLSISALVQARAARAELAALAAET
                     RQVMGELARLRPRPPPAEAVAPPPPPPPPPPPPPPPRPASQAAPAPAGGRPGDGFEKS
                     LTSRWLVWTGAVALALAGVFLVKYSIEKGWLSPPVRCAAGVVLGALLVVAGEWLRRQP
                     LQRAIAAVQANYIPPALTAAGVATLYASMYAAHALYALIPGVVAFILLAIVSALAFGL
                     ALLQGALIAVLGVIGAMAVPALVSTGQDSALALFSFLVAVVGAALWILRYRAWWQVAW
                     LALAGAALWPLGWFMFAWHPADSPVMAIYLLAAAAMFLLLAPPQARAATPAPASGFMG
                     LAASDPVALAAALAFALLTFVLVRVDGYEAPSLIALAALSVLYMAVGRRHVRFDVLAV
                     VAFAVMLALVALWHLPQVSDTAGRTLGVAGWRYDLGRGAIVPPALLPFAIVTSLFAAL
                     FAGGGYIALWGARRPAVWAHVSAAAPVLVLAIAFWRVQKFEIDLAWAAAGLVVAGVEL
                     LAAERVARQRDAQGMAGVLAAYAMGVAAGLGLALAMALRQAWLTAALALLVPALAVIH
                     DHLPLAAIRRVAWIAAASVTVRLVLNYRILDYDPGPLPGIGWMAYGYGVPMVAFAYAA
                     IRFRRRGDGPLVHLLEAGAIAFAALFVTLEIRQLITGDISLPRYSLAERALQSAAWLA
                     LAWGLYRRHQVAPRPVLGWAWRVLAALGAAHVVLIQVLFANPLFAPAAVGDWPVMNLL
                     LLAYGVPALIAFLFAGTARGLAPAAGGEAAPPRPEARWLVPLGGGAGLVLLFVWLSLE
                     VRRAFHGSVLASGVIDGQYVTVPPTSDAEWYAYSVAWLAYAAALLALAIWREAAALRY
                     ASLALVMLTVAKLFVFDMSELTGLYRVASFLGLGLALVGIGYLYQRYVFPPRRPAVPA
                     G"
     gene            complement(11841..12779)
                     /locus_tag="VGB88_04250"
     CDS             complement(11841..12779)
                     /locus_tag="VGB88_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646684.1"
                     /translation="MTGPLRTAALAASLLALAAAAAAAGEGTPGLPNLALPTLGGKQL
                     WVDRFIYAGWRIQENALTGHHRLLDRDDVRRAWGGYGECLAGFLDLRAETSLRPRSRH
                     LVLMIHGLGRSKDSFAALEAALLAAGYEAAGFNYPSLLGGLDRHAADLVGVLDRLAGA
                     DKVSFVTHSMGALVLRAALARDGDWRRRLALGRIVLIAPPNQGSAVAALVDSIPPLEW
                     IGGHALGDLTPAAVEALPGLAGEFAIIAGGKGDGKGFNPLIAGDDDGTLAVAETRLAG
                     VADMLLVRSLHSFVQGHPDTIAAVLSFLARGRFAGE"
     gene            complement(12776..13402)
                     /locus_tag="VGB88_04255"
     CDS             complement(12776..13402)
                     /locus_tag="VGB88_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9646685.1"
                     /translation="MHSGRCSASSSTSAWAAAGLAVAAVELAAAERIARHRAACGMAG
                     TLDDVPQATFHLQDAEMIYATGRHMAHTPFGYAYSPDHIARMVAAVFKGRVAFHDGEA
                     EVAPGVTVHHIGGHTLGMQCVRVLTKGGWVVLASDASHFYENMEATSPFPIVYSVADM
                     VEGYAKMRALATSPAHIIPGHDPLVMDRYPAPKPDVGEVVVRLDVAPR"
     gene            complement(14103..14906)
                     /locus_tag="VGB88_04260"
     CDS             complement(14103..14906)
                     /locus_tag="VGB88_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9646686.1"
                     /translation="MSDPEAYEVFAIRYGRHQKRTRGENFIVTDIHDSPMPLDYYVWA
                     VVNDRRAIVVDTGFDFEEAKRRNREIVRLPREGLAMIGVDSAKVADVVVTHLHYDHAG
                     TLDDFPAATFHLQDLEMAYATGRHMAHTPFGYAYSPRHVGRMVEHVFKGRVAFHDGNG
                     EVAPGVTVHHVGGHTRGMQCVRVLTRRGWVVLASDASHFYENMEGTAPFPIVYSVADM
                     VEGYAKMRGLATSPAHIIPGHDPLVMDRYPAPKADVAGVVVRLDVAPRA"
     gene            complement(14917..16131)
                     /locus_tag="VGB88_04265"
     CDS             complement(14917..16131)
                     /locus_tag="VGB88_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646687.1"
                     /translation="MTRLCVARLASAAIALGLAAGSVAAEPKYGPGASDTEIKVGNTN
                     PYSGPASAYGAIGKAIGGYFDMVNANGGINGRKVNFISYDDGYSPPKTVEQIRRLVEQ
                     DEVLLLFQTLGTPTNSAIHKYVNSRKVPHIFIATGATKWGNPKEYPWTMGWQPPYSDE
                     SKIYAKYVLDNVDDPKIAVLYQNDDYGKDYVDGFRAGLGELADKLIVAEAPYEVTDPT
                     IDSQIVEMQASGANVFFNVTTPKFAAQAIRKVYDIGWRPLHLLNNVSNSVEAVLKPAG
                     LDKSVGLISSFYLKDPSDPKWGNDADVKTWDAWMDKYNSGANKAEVFNAYGYAVAATL
                     EATLRQCGDDLTRENVMRQAASLDHLEVPMLLPGITVTTGTDDFYPLQQLQLSRFDGT
                     SWQPIGEVMNAF"
     gene            complement(16161..17162)
                     /locus_tag="VGB88_04270"
     CDS             complement(16161..17162)
                     /locus_tag="VGB88_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9646688.1"
                     /translation="MTGAGNARRLGLAAALAAAAALAFVLSDYRLFQVTLALIYAIAI
                     LGLNVLTGRCGQISLGHGAFYALGAYTAAILMDRAGVAYYLTLPVAFVVCLAVGFLFG
                     FPAGRLRGHYLALATFALAVSVPQILKYDPLEPWTGGVQGIFIFKPEAPFGLALSADQ
                     WLYLFTLAVAVVAFALAHNLLRGRSGRAMAAIRTHLIAAEAMGVDTGLTKSLTFGVSA
                     AYTGLAGALGAIAVEFVGPDSFGFLVSILFVIGMVVGGLGSIWGSVVGGLFIVFVPNL
                     AEQVSKAAPWAIYGIFMIGMMYLMPKGAAGFVTMAGGRIARRLARRAPAGPGPAARP"
     gene            complement(17167..18051)
                     /locus_tag="VGB88_04275"
     CDS             complement(17167..18051)
                     /locus_tag="VGB88_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9646689.1"
                     /translation="MDLFIQLVVAGLAAGGIYASIALALVMIHEATELINFAQGEMAM
                     FSTYVAWTLIQAGMPFWAAFFITVAASFAGGVAIERVIIRPFEKASILTLIIVFIGLL
                     VIFNSLAGWIWTYTIKSFPSPFPEAPFALGAGVSLRMHDVGAIGVTLAVLVLLYLFFR
                     FTPLGLAMRAAAQNPTSSRLVGIRVGWMLALGWGLAAAIGAVAGMMVAHSVFLDPSMM
                     SGVLLYGFAGALLGGINSPGGAVAGGFVVGVLENLVGAYVPFIGNELKLTVALAIIVA
                     VLVFRPGGLFGRVAVTRV"
     gene            complement(18253..18966)
                     /locus_tag="VGB88_04280"
     CDS             complement(18253..18966)
                     /locus_tag="VGB88_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646690.1"
                     /translation="MAALLEARGLTAQYGPVQVLFGIDFGVAEGGISTILGANGAGKT
                     TALRALCGMVRAGGEIAFDGARIDGRATEDLVRLGIGHVPDGRGTFTALTVEENLRLG
                     AFTRRDRAGARRDAERVYDYFPRLGERRRQQAGTLSGGEQQMLAIGRALMLRPRLMLL
                     DEPSFGLAPLVVQGLFDILRTINREQRIGMLLVEQDAGLALELAQDAYLIETGRVVMA
                     GPSAALKADESVRRAYLGY"
     gene            complement(18966..19760)
                     /locus_tag="VGB88_04285"
     CDS             complement(18966..19760)
                     /locus_tag="VGB88_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646691.1"
                     /translation="MPAARGRTQDGPLLEVERLSIRFGGIVALDQVSFAVHPGQIVAL
                     IGPNGAGKTTLFNCLSRLYHPDSGQVRFAGTDLLARPAHRIARLGIGRTFQNLALFEG
                     VSVLDNVLIGAHCRCRADFLSDALRLPWVRREEAALRAQAMEILDFLDLGPLASEPVE
                     SLAFGTRKRVELARALAGRPRLLLMDEPAGGLNHEEVQRLGALIKDIRDARALTILLV
                     EHHMGLVMPISDWVVALDFGRKIAEGTPAQVQRDEAVITAYLGTGG"
     gene            complement(19854..20402)
                     /locus_tag="VGB88_04290"
     CDS             complement(19854..20402)
                     /locus_tag="VGB88_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX9646692.1"
                     /translation="MRARFALRFRPGTLAAHATGHDRLVGLWLVLAAALLGAGLFLPA
                     VTVRKMFVLAREYSLAEAVFAFATAGDWFLFLITFAFTIAFPVAKLVACLALWFAVPH
                     AGARGGQLAGALAGLSKWSMLDVFMIALVVLVVDGQLLSSADVHVGVIAFAAAVVAST
                     YGARRIAVLLARPEGAALRAGS"
     gene            20644..>21019
                     /locus_tag="VGB88_04295"
     CDS             20644..>21019
                     /locus_tag="VGB88_04295"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9646693.1"
                     /translation="MKRSAGLGAVRRALAHPSYRSYTAGNAVSLIGTWIQRIGVGWLT
                     WQLTGSPTWLGLIAFADLAPAVVVGPLGGVLADRVDRLALIRLMQTCSMLQALALFAL
                     TALDAISIGLLLVLVLINGIVIG"
BASE COUNT         3389 a         7485 c         7302 g         2843 t
ORIGIN      
        1 ccagcgggtc gacgccgcca tggcccagaa ggccgcggcg cacaagctgg ccgagctgtc
       61 gtggccgggc taccgcaagt tcgagatcgc gcgcaaggcg gccgaggccg acgccatcgc
      121 ctcgttctat ctggtccccc acaaccgcac gccgctgccg ccatacaagc ccggccagta
      181 cctgaccttc cggttccaga tcccgggccg ggccaagccg ctgatccggt gttactcgct
      241 gtcggacgcg cccgacggcg accagtaccg cattaccgtc aagcgcctgc cgccgccgca
      301 cgagcccgcc gggctgccgc cggggctggc atcgtcgttc ctgcacgatg cggtgtcgca
      361 cggccacctg ctcgacgtca tggcgccgac cggtcacttc ttcctggacg aggccagcga
      421 gcggccggtg gtgctgatcg gcggcggcat cgggctgacg ccggtactcg ccatgctcaa
      481 cgccatcgcg cggtcgggac ggacgcgcga gagctggctg ttctatgcgg tccacaaccg
      541 gcgccaggag gtgatgggcg agcacctgcg cgagatcgcc agcggcaacc ccaacatcca
      601 cgtccgcatc gcccacagcc gccccgacca ggacgagcag gcggggcgcg attacgacta
      661 ccccggccac ctcaccatcg acgtgctcaa gcgcgagctg ccgtcgaaca acttcgagtt
      721 ctacgtctgc ggcccgccgc cgatgatgag caagatgatc gcggacttgc gcgcctggca
      781 ggtgcccgag gagcgcatcc acttcgaggc gttcggcgcc gccaccattc agcagatcgg
      841 cgcggcgaag gacggtgccc agagcttcga ggtggtgttc gagcgctccg gcaagacgct
      901 gacctggcgg gccgaggcgg gcaccatcct cgacctcgcg gaaaagaacg gcgtcgccat
      961 cgactacggc tgccgcgccg gcaactgcgg cacctgcgtg accgcggtgc gcgcgggcga
     1021 gatcacctat ctcggcgagc cgggcgcgag cgccgagagc ggcacctgcc tgacctgcat
     1081 ctcgatcccc aagacccgcc tggttctgga cgcctgaggc gccttacggc gacatttgct
     1141 tcgcgcgtca ctgtgctatc ctgtggctcg acttggccaa tcgggagcac cggcggtccg
     1201 gcgccggtca ccgggattcc tgcgggaggc agggggcgat ggcatcgatc atgatgggca
     1261 agctggtcgg atggacgcac aggggggcgc tcgcgggcat cgctttgatg gcgctcgcgg
     1321 ccaccggctg ggcgggcccc gatccggcca aggtgatcgg ccccgacgag tgcggcgaat
     1381 gccacaaggc cgaggtcaac gtgtggcgca agacccacca ttacaccacc ttccaggagc
     1441 tgccgcgcaa ggacaaggcg cgtgagatcg ccgacaagat ggacgtccgc cgcctcaagt
     1501 ccgagagcct gtgcctgaaa tgccacttca ccgagatgac caaggacgac ggctcgaccg
     1561 acaccatcgc cggcatcggc tgcgagtcct gccacggcgg cgccaaggac tggaacaaga
     1621 tccacagcga ctatggcggc aaggacgtca agcgcgactc ggaagccccc gagcatcgca
     1681 cccagcgcat cgcggcatcg cgggcggcgg gcatgatcca gccgtcgatg ctctacgact
     1741 gggccaacaa ctgctacagc tgccataccg tgcccgagga gaagctggtc aatgtcggcg
     1801 gccataccgc cggcagcaag ttcgagctgg tgacctggac gcagggcgag attcgccaca
     1861 acgtctggta caccgacgcc aagtcgaacc cggaggcctc ccaggagcag aagcgcctga
     1921 tgctggtggt cggccagatg ctcgacctcg agcatgccct gcgcggggtg gcgaaagcca
     1981 ccgagcgcgc cgactacgcg gtcgccatgg ccgggcgcgc caaggccgcg gcccagcgca
     2041 tcaagcagat ctcggaggcc cagaccacgc ccgagatcga cgccgtgctc gcggtcgcag
     2101 ggtcggtcga gctcaagctc ggcaacgccg atcagctcac cgccgccgcc gacaagatcg
     2161 gggagcaggc gcgcgcgctc gccgccaaca acgacggcag cgcgctcgcc ggcctcgacg
     2221 gcctgatccc gggcgcggac cagtacaagg gtacgccgtc gcaatagccg gggcgaacgc
     2281 gatgcgaggc cggccggccg gcgatcgccg cggtcgcgcg agaggcgaca ggagcgcacc
     2341 gcgcaccgcc tgatcagtgc gcgggcgaga gcaagtacat ggccaggcca cgagtcgagc
     2401 tgcaccgtcc cgagcgatgg gacaacccgt tcagccccga gatgacggcc gagcaggtcg
     2461 agcgcctgct cgccatgccg ccgttcgacg agatcgacgc gagcaagttc ccgccgacca
     2521 actcgctcgc cgacatcgtg cgcaacgaca cccgcatccg cgacttcgcc cccggcgaca
     2581 tcgtggtgcg cgaggcggac tacggcaact cggccttcgt cattctcgac ggccgggtgc
     2641 gggtgatcca ggcaccgcct ctgccggcac aggcgctggg ccgccagccg ccgcgccggc
     2701 gcagcccgtg gcaggcgctg gcccagctgt ggaccaaccc gcgcaccgcc gagcggcgcg
     2761 acgcggcgcg ctaccgcggc gatgcgcagg tgcgcctgac ccgcgccggc gaggacgagt
     2821 tccgcgtcac cctggcggac cctgacggcg tgatcgcgcg ccatcgcacg gcgcagatgg
     2881 gccccggcga gatgttcggc gagatcgccg cggtcgcgcg cggcgcacgc gccgcgacgg
     2941 tgttcgccga gacgcgcgcg accctgctcg agattcgctg gcaggggctg cgcgacatgc
     3001 gccgccgcga tgcacggttt cgcgacaagg tcgacgagct ttatcggaag cgggcgctgg
     3061 gcgcgcatat gcgcgaatcg ccgctgttca agggcctcga cgacgagccg atgaaggagg
     3121 tctcccgcac cacccagttc gcgagcttcg gcgagttcga ctggcacaca tcgtacaagg
     3181 cactggccgg caagaccgcg ccggagcgcc tcgaggacga gccgatcatc gtcaccgagg
     3241 accaggtcgc ggacgcgctg atcatggtcc gctcgggatt cgcccgggtc agcgagcggg
     3301 tcgatcacgg ccaccgcacg gtcaactatc tggggcccgg cgacgtcttc gggctggccg
     3361 agatcgtcta caactggcgc aacgacgccc acatcaccta tcagcacacc ttgcgcgcca
     3421 ccggcagcgt cgaggtcctg cgagttccgg cgcaggtgat cagggagcac gtccttccgg
     3481 gtctgccgcg cgcgctgctg ccgccgccga ttatcccgcg ctccggcgcg cgctcggcgt
     3541 ggcaggcgga cagggtagcc gaggacattc cgcccgcgat gctcgagttc ctggtcgaca
     3601 accggctgat caacggcacc gcggcgatgg tcatcgacgt cgaccgctgc acgcgttgcg
     3661 acgagtgcgt caaggcctgc gccgcgaccc acgacaacaa cccgcgtttc gtgcgtcacg
     3721 ggctgctgtt cgatcacttc atggtcgcca atgcgtgcat gcactgcgtc gatccggtgt
     3781 gcatgatcgg ctgcccgacc ggcgccatcc accgcaacgt gcaggccggg caggtggtga
     3841 tcaacgacga tacctgcatc ggctgcggtt cgtgtgccaa cgcctgcccg tacaacaata
     3901 tccgcctggt cgacatccgc gacgacgacg gcaacttcat gctcgacgag gccaccaaca
     3961 tgccgttgga gaaggcgacc aagtgcgacc tgtgcgcgga ccagatcggc ggcccggcct
     4021 gccagcgcgc ctgccccaac gacgccctgg tgcggatcaa cctgcgcgac ctcgaccgac
     4081 tggccgagtg ggtcgggcgg tgaggtactt cgccgtccgg ctgcggcgca acctggcgct
     4141 gaccggcgcg gcgtgcctgg cgctgctcat ggtctacgcg gtccacggcg caagcctggc
     4201 gcatgagaac ttcgtcaccg gctggctgct ggtcgcggcg atcgccgtgc tggtgctgta
     4261 caacgcccgc cgcggggcgc ccgcgttgcc gctgggctcg tcgacggcct ggatgcagct
     4321 ccacgtctat ctcgccatcg tggcgatctt cctgttcctg cttcacgtcg gcatcgaggt
     4381 gccgcgcggg atcatggaga aggtcctggc cgggctgttc gtcgcggtgg cgggcagcgg
     4441 cgtgttcggc ttcgtgctcg cgcgactgct gccggcgagg ctgtcgcggc ggggcgaggt
     4501 gatgctgtac gagcgcatac cccagttccg ccgccagctg ctcgagcgcg gcgaggcgct
     4561 ggcgctgcgg gcggtggagg aaaccggctc gaccactatc gccgattact acgccgaccg
     4621 gctgcggccg tttctcgacg ggccgcggaa cttctgggcg cacgtgctcg aggtctcgcg
     4681 cgggcgccgg gacctgctgg tcgagatgcg cgatctggag cgctatctcg acgacaacga
     4741 gaaggagatc ctgcgcgagc tggccgagct cagcgggctc aaggacgacc tcgactacca
     4801 gtacgcgctg caatcgatgc tcaagcgctg gcagttcgtc cacgtgccgc tggcctacgc
     4861 cctgatcgtc ttcgtgctgc tgcacgcggt cgtggtctac gctttcgcct gacatgccga
     4921 cgtcgaagcg cctgctcaat ttcggccacc gggaaagccc ttacgaccgg cccaaccaga
     4981 gctgggtgtg cggccacctg gtcgacggcc gcgcctgcgc catcgggccc gacaagcgcg
     5041 ggcggtgccg ggccaccgcc gagtgcctgc cgatccgcaa ggacgatcgc tggcaatgcg
     5101 cgcggccggc gcaacatggc gggcgctgca ccgagggccc ccaccccgat ggcggatgcg
     5161 gccgcccgat cgcgacctgc cagccggtgc gcagcctggc cgcgcggcgc gccgtggcgg
     5221 tgcgctggac gctgatcgcc tcgctcggcc tggtgctgct ggtcggctcc ggcgtcagcg
     5281 gccgcgagtt cgttttctcg ggcgagctca ccttccagca cggcgaggtc ggccgctgca
     5341 cgggctgcca ttcggtgccc gatcacggcc cgatctcgtg gctggcggcg gcgttcaggg
     5401 accgcgcctc gatcgacgac agcggcctgt gcttgaagtg ccaccggctg ggcgaggatg
     5461 cgctggccgc gcacgacttg cctcgggcga cggtgagcgc gatcaccgag cgccagtcgg
     5521 cgaaggcggc gcaggcgccg ctgctgctca agctcgccaa gctgacgatc ggcgcgcccg
     5581 ccggcgacca gccggttgcc tgcggcacct gccatcgcga gcacaacggc cgccgcttcg
     5641 acctgaccgc gatgagcgac ctgcgctgcc agacctgcca ccgcgggcag tttgccagcc
     5701 tggccgacgg ccacccgcgg ttcgagggct tcccctaccg gcggcgcacc cgcatcgcgt
     5761 tcgaccacgc ctcgcacatc ggcaagcatt tcaaggcgga ctcggcgatc gacgcgccca
     5821 cctcgtgcac cggctgccac gtgctggagc ccgccggccg ggccatggag gtcaagccgt
     5881 tcgaggtcag ctgcgccgcc tgccacctgg gccagatcat gggcgagggc cgcgccggca
     5941 ccaaaggcta tccggtgctg atcctgcccg ggttcgatat cgagaccctg tacgcgcgcg
     6001 ggcacgaggt gggcgactgg ccgcgcgacg ccgacggcaa gctgacctcg ttcatgaacc
     6061 tgctgctggc caacgatccc gcgcatgccg aggacctgtt cgtgctgtcg ggggtggacc
     6121 tgatggacct gcgcgaggcg accgaggagc agctcgacgc cgccgcgcgc accatctggt
     6181 cgatcaagga cctgatctac gatctggcgg tcaccggcca cgaggcgtta cgcgcgcgag
     6241 tcgccggcgc gctgtcggtc gaggtgccgc gctacgagtc gggaccgctg atgggaacca
     6301 tgccggtcga cgcggtggcc tcggcccagc aggcgtggtt cccgcgtctt gcgggcgaca
     6361 tcgagcgtca tcgcgcgggc gagccggtgc cgccgcccga ctggatcgcg agccccgcgg
     6421 gcgaggcctc gacgccggtc gcgccggccg atgcgggcgc gccggcgccg gcggatcagg
     6481 acctcggcgg cgacctgctc ggcggcgacg agggcgggct gcttggaggt gacgaagggg
     6541 gtcttctcgg cggcggcgag ggcgggctgc tttcggagcc ggcacccgag ccgggtgcgg
     6601 gccaggagca ggtcgaggcc gccgagcccg ccgccgtacc ggaaagcatg atgcccgagg
     6661 acatcgaagg ctggatgcgg gccggcggct ggtaccgcca gggctatgcg ctgctgtacc
     6721 gtccgatcgg gcatcgcgac ccgttcatgc gcgcctggat cgacctcacc gccggcgtcg
     6781 ggccgtcgcc gggcgcgccg cacgcgttgc agatcttcgc cgagatcacc tcacgcaagg
     6841 cacccggcat gtgcggcaag tgccacagcg tcgacgccac cgccgacggc accacggtgg
     6901 tcaactggcg gcccaagcag cggccgcagg acgtcaatcg gttcaccgag tacgcgcacg
     6961 cgccgcattt cagcctgctc gacgagaaag gctgcctgac ctgccacgtg atcgactacg
     7021 aggccgactt catggccggg ttcaacgacc tcgacgccag caccttcgcc agcaacttca
     7081 agccgatgaa taaggagctg tgcgcatcgt gccacgtcga ctcgttggcc ggcgacgcgt
     7141 gcaccgactg ccacaactac cacatcggcc ggttcccgcc ggcgctggcg gcggcgccgc
     7201 tggcggtgga cgcgggcgca ggcgcgcctg ccccgcacga ctagttgccg ggcgcctgcg
     7261 ccgcctgcat cgtctcgatc tgcttcttca ggtcgttgat ctggcgctgc agcagcatga
     7321 tctgcttggt caggcggatg tccatctcgg tggaggtgtc gaggacctcg cgaatgtcga
     7381 gctcgccgtc gccggtgacg cctggaacgt cgcccaactc tttggtatcc acgggcatgg
     7441 caggcgcagt gccgccggcc agggcgagcg ccacggccag caccaccacc gccggccggg
     7501 cgacgtgcgc gcgtcggccc ctgccctgtt cgatcggtct cgtcctcgcc accgtgagca
     7561 ctcccctgct ggccgatgtt gccgccgggc cgcaacgccg cgcgccggcc cgaggcagtc
     7621 tagcgcgatg gcggggcgcg agaaagggcg cgcccggagc caaggccggc aacgagcgat
     7681 attgacgccg aggggccggt ccgccgaagt ttggcggcag cggacgggga caagggatga
     7741 gcgcagcgaa atcggggtcc cagccgcggc gacgcaagcg ccgcgcgata cggctcaggc
     7801 gccgcagcct gcccggcgcg gcgcccgggg tcctggtcgc cgatcccgag gcgcgcgaat
     7861 ccgaggtcaa tgtcatcctc tacggccctg acgggctgga agaggttcgc ggctgcccgc
     7921 cgtcggagct gcgcggcctg cgcgccaggg cgaaggtgct ttggatcgac gtcaccggcc
     7981 ttgccgacct cgcggtgatc gccgagatcg gcgcggcgtt cgccatccac ggcctggcgc
     8041 tcgaggacgt ggtcaacgtc caccagcggc cgaaggcgga gcagttcgag gaccacgtgt
     8101 tcctggtcgc gcgcatgctg gccgcggaag gcagggccga gaccgagcag gtctcggtgt
     8161 tcctcggcga ggactatctg atcagcttcc aggagcggcg gggcgactgc ttcgagccga
     8221 tccgcgagcg catccgccgc ggcaaggggc gcatccgcga gatgggcgcc gattatctgg
     8281 cctatgcgct gatcgacgcg gtgatcgaca gcttctaccc ggtgctggag ggctatggcg
     8341 aggacatcga gcgtctcgag gacgacgtga tcgccggacc gagcccggat caggtcgacc
     8401 gcgtgcatac ggtcaagcgc gacctgttga cgttgcgccg cgcgatctgg ccgacgcgcg
     8461 agatggtcaa cggcctgatc cgggatgaaa gcccgttcgt cggcgccacc acccgcgtct
     8521 acctgcgcga ctgctacgac cacgccatcc agcttatgga catcgtcgag acctatcgcg
     8581 agatcgcgac cggcttgctc gacgtctatc tgtcgagcat gaccgcgcgg ctgaacgaga
     8641 tcatgaaggt gctgaccatc atcgccacca tcttcatccc gctcagcttc gtcaccggca
     8701 tctacggcat gaacttcgac cgcgcggcct cgccgtggaa catgcccgag ctcggctggc
     8761 gcctcggcta tccgatggcg ctcgcgatca tggtcgcgat cgccggcgga ctgctgttct
     8821 acttctggcg caggggctgg ttggccaagc ggcgatgagc cggccctact tttagccagc
     8881 cggcaccgcg ggccggcgcg gcgggaacac gtagcgctgg taaaggtaac cgatgccgac
     8941 cagggcaagg ccgaggccaa ggaacgaggc gacgcggtag agcccggtga gctcggacat
     9001 gtcgaacacg aacagcttgg cgacggtgag catcaccagg gcgagcgagg catagcgcaa
     9061 cgccgccgcc tcgcgccaga tggcgagcgc caacagcgcc gcggcgtaag ccagccaggc
     9121 gaccgaatag gcgtaccact cggcgtcgct ggtcggcggc acggtgacgt attggccgtc
     9181 gatcacgccg ctcgccagca cgctgccgtg gaaggcgcgg cgcacctcga gcgacagcca
     9241 cacgaacagc agcaccagcc cggcgccgcc gccgagcggc accagccagc gcgcctcggg
     9301 ccgcggcggc gccgcctcgc cgccggccgc gggcgcaagg ccgcgcgcgg tgcccgcgaa
     9361 caggaaggcg atcagcgccg gcacgccgta ggcaagcagc agaaggttca tcaccggcca
     9421 gtcgccgacc gccgcgggcg caaacagcgg gtttgcgaac agcacctgaa tcaggacgac
     9481 atgcgcggcc cccagcgcgg ccagcacccg ccacgcccag cccagcaccg gccgcggcgc
     9541 cacctggtgg cggcggtaga ggccccaggc gagggcgagc caggccgccg actgcaacgc
     9601 gcgctcggct aacgaatagc gcggcagcga aatgtcgccg gtgatcagct gcctgatctc
     9661 gagggtgacg aacaaagccg cgaacgcgat cgcgccggcc tcgagcaggt gcaccagagg
     9721 gccgtcgccg cggcggcgga agcggatcgc cgcataggcg aacgcgacca tcggcacgcc
     9781 gtagccgtag gccatccagc cgatccccgg cagcgggccc gggtcgtagt cgaggatgcg
     9841 gtagttgagc accagcctga ccgtcaccga cgccgcggcg atccaggcga cgcgccggat
     9901 cgcggcgagc gggaggtggt cgtggatcac cgccagcgcc ggcaccagca gcgccagcgc
     9961 cgcggtcagc cacgcctggc gcaacgccat cgccagcgcc aggccgagcc ccgcggcaac
    10021 ccccatggca taggccgcga gcaccccggc cattccttgc gcatcgcgct ggcgcgccac
    10081 gcgctcggcc gccagcagct cgacgccggc gaccaccagc ccggccgccg cccaggcgag
    10141 gtcgatctcg aatttctgga cgcgccagaa cgcgatcgcc agcaccagca ccggcgccgc
    10201 cgccgagacg tgcgcccaca ccgccggccg gcgcgcgccc cacagcgcga tgtagccgcc
    10261 gccggcgaac agggcggcga acaggctggt gacgatcgcg aacggcagca gcgccggcgg
    10321 cacgatcgcc ccgcgcccga gatcgtagcg ccagcccgcc accccgagcg tgcgcccggc
    10381 ggtgtccgag acctggggca ggtgccagag cgcgaccagc gccagcatca ccgcgaacgc
    10441 gaccacggcg aggacgtcga agcgcacgtg gcggcgcccg accgccatgt agagcaccga
    10501 cagcgccgcg agcgcgatca gcgacggcgc ttcgtagccg tcgacccgca ccagcacgaa
    10561 ggtgagcagc gcgaacgcca gcgcggcggc gagcgcgacc gggtccgagg cggcgagccc
    10621 catgaacccc gacgccggcg ccggcgtcgc cgcgcgcgct tgcggcggcg cgagcagcag
    10681 gaacatcgcg gccgccgcca gcaggtagat cgccatcacc ggcgaatccg ccgggtgcca
    10741 ggcgaacatg aaccagccga gcggccacag ggcggcgccg gcgagcgcga gccacgccac
    10801 ttgccaccac gcccggtaac gcaggatcca caacgccgcc ccgaccacgg caaccaggaa
    10861 cgagaacagc gccagcgccg agtcctgccc ggtcgagacc agcgcaggca ccgccatcgc
    10921 cccgatcacg ccgagcacgg cgatgagcgc gccttgcagc agcgccaggc cgaaggcgag
    10981 cgccgagacg atggcgagga ggatgaacgc gaccacgccg gggatcagcg catagagcgc
    11041 gtgggcggcg tacatgctgg catagagcgt cgccacgccg gcggcggtca gcgccggcgg
    11101 gatgtagttc gcctgcaccg cggcgatggc gcgctgcaac ggttggcggc gcaaccattc
    11161 gccggcgacg accagcagcg cgcccaggac cacgcccgcg gcgcagcgca ccggcgggct
    11221 cagccagccc ttctcgatcg agtacttgac caggaacacc ccggccaacg ccagcgccac
    11281 cgccccggtc cacaccagcc agcgcgaggt caggctcttc tcgaagccgt cgcccggccg
    11341 gccgccggcg ggcgccggcg cggcctgcga ggcgggccga ggcggcggcg gtgggggcgg
    11401 cggcggaggt ggcgggggcg gcggcgcgac cgcctcggcc ggcggcgggc ggggccgaag
    11461 ccgcgcgagc tcgcccatca cttggcgcgt ctcggcggcc agcgccgcga gctcggcgcg
    11521 tgcggcgcgc gcctggacca gcgccgagat gctcagcccc ggcaccacca gcagcgcgaa
    11581 caccgcgatc aggatggcaa ggaccgccca ggaatccatg gctcgatcac cggccgcgcc
    11641 gatccctcat gccggccacg gcgccatgcg ccgatggccc ccggtgcccg ccccccgccg
    11701 aacgcgcttg ccgtcccatc tgcgccgacc cccggtcggg ttgctcacga agtcgaaact
    11761 gatcgtcgtt cagtgtcatg gtgccgcacg atccgaatgt caacatattt ttgatcaatg
    11821 ttcataatcg cccggccacc tcactcgccg gcgaagcgcc cacgggcgag gaacgacagc
    11881 accgcggcga tggtgtcggg gtgcccctgc acgaacgagt gcaggctgcg gaccaagagc
    11941 atgtccgcga cgcccgcgag gcgggtctcg gccaccgcca aggtgccgtc gtcgtcgccc
    12001 gcgatcagcg ggttgaagcc tttgccgtcg cccttgccgc cggcgatgat ggcgaactcg
    12061 ccggcgagcc cgggcagcgc ctcgaccgcc gccggcgtga ggtcgccgag cgcgtggccg
    12121 ccgatccact cgagcggcgg gatcgagtcg accagcgccg ccaccgccga gccctggttg
    12181 ggcggcgcga tcaggacgat gcgcccgagt gcaaggcgcc ggcgccagtc gccgtcgcgc
    12241 gccagcgccg cgcgcagcac cagcgcgccc atgctgtggg tgacgaacga caccttgtcc
    12301 gcgccggcca gccggtcgag cacgccgacc aggtccgccg cgtggcggtc gagcccgccg
    12361 agcaggctcg ggtaattgaa cccggcggcc tcgtagccgg ccgcgagcag cgccgcctcg
    12421 agggccgcga acgagtcctt cgagcgcccg agcccgtgga tcatcagcac cagatggcgg
    12481 ctccgcggcc gcaagctggt ttccgcccgg agatcgagga acccggccag gcactcgccg
    12541 tagccgcccc acgcgcggcg cacgtcgtcg cggtcgagca ggcggtgatg gccggtcaac
    12601 gcgttctcct ggatgcgcca gccggcatag atgaaccggt cgacccagag ctgcttgccg
    12661 cccaaggtcg gcaaggcgag gttgggcagg cctggggtcc cctccccggc cgccgccgcg
    12721 gcggcggcaa gggcgagcag cgatgcggcg agcgcggccg tccgcaacgg gccggtcatc
    12781 gcggcgcgac gtcgaggcgc accaccacct cgcccacgtc cggcttcggc gccgggtagc
    12841 ggtccatcac cagcgggtcg tggccgggaa tgatgtgcgc cggtgaggtg gcgagcgcgc
    12901 gcatcttggc atagccctcg accatgtcgg cgacactgta gacgatcggg aacggcgagg
    12961 tcgcctccat gttctcgtag aaatggctcg cgtctgaggc cagcaccacc cagccgccct
    13021 tggtgagcac gcgcacgcac tgcatgccca aagtgtggcc gccgatatgg tggacggtga
    13081 ccccgggggc gacctcggcc tcgccgtcgt ggaacgcgac ccgccccttg aacacggccg
    13141 cgaccatgcg cgcaatgtgg tccggcgagt aggcgtaacc gaacggtgtg tgcgccatgt
    13201 ggcggccggt ggcgtagatc atctccgcgt cctgcaggtg gaacgtcgcc tgcgggacgt
    13261 cgtcgagggt gccggccatg ccgcacgccg cacggtggcg cgcgatccgc tcggccgcgg
    13321 cgagctcgac ggcggcgacg gccaggccgg ccgccgccca agctgaggtc gagctcgaag
    13381 cgctgcacct gccagaatgc aatcgcgagc acgtgcaccg gagcgcccgc cgagacatgc
    13441 gcccacagcg cggggcggcg cgcgccacac agggctgcga agccgccgac ggcgaacagc
    13501 gccccgaaca ggctggtgac catcgcgaac ggcaccagcc ccggcgccag gatcggcccg
    13561 cgcccgaggt cgtagcgcca gtccgcggcc ccgagagatc gcggcggcgg gcgcgttcgc
    13621 ggctctcacg gtgcgcgtaa acggggacga ggcgccgtcg ctgatcctgc tcgcatcgct
    13681 gttggcgccc tacatggcat tcgggcgccg ctacgtgcgc tttgacttgc tcgcggtggt
    13741 ggcgtttgct gtgatgctgg cactgttcgc attctggcac ctgccgcaga tctccgacac
    13801 cgtcgggcgc aggcccagtg gctctcgatc cacggcaacc ccacgcccac ggcgaacatg
    13861 aaggcgacca gcgtcagcgc caactcctcg tgtccggtcc tggctagctt ctggaccagc
    13921 gccagcgcca ggtggagcaa caagataaag agcgtttcca tccccgtatc accggccgcc
    13981 ccggagcgtt aattgatcag cgttcagtaa catgcaatcg gcccgcgcgg aagtcaagct
    14041 ttttttgatc ggtgttcaat tttagcccgg caacaagccc ggctatgacg ccttcgcgcc
    14101 gcctaggcgc gcggcgcgac gtcgaggcgc acgaccacgc ccgcgacgtc cgccttgggc
    14161 gccgggtagc ggtccatgac cagcgggtcg tggcccggaa tgatgtgcgc cggtgaggtg
    14221 gcgaggccgc gcatcttggc gtatccctcg accatgtcgg caacgctgta gacgatcggg
    14281 aacggcgccg tcccttccat gttctcgtag aaatggctgg cgtcggaggc cagcaccacc
    14341 cagccgcgcc gggtcagcac gcgcacgcac tgcatgcccc tggtgtgacc gccgacgtgg
    14401 tggacggtca ccccgggagc gacctcgcca ttgccgtcgt ggaacgccac ccgtcccttg
    14461 aacacgtgct cgaccatgcg cccgacgtga cgcggcgagt aggcgtagcc gaacggcgtg
    14521 tgcgccatgt gccggccggt ggcgtaagcc atctcgaggt cctggaggtg gaaggtggcc
    14581 gccggaaagt cgtcgagggt gccggcgtgg tcgtagtgca gatgggtgac gaccacgtcg
    14641 gcgaccttgg ccgaatcgac cccgatcatc gccaggccct cgcgcggcag gcgcacgatc
    14701 tcgcgattgc gccgcttcgc ctcctcgaag tcgaagccgg tatcgacgac gatggcgcgc
    14761 cggtcgttga ccaccgccca gacgtagtag tcgagcggca tcggcgaatc gtggatgtcg
    14821 gtgacgatga agttctcgcc gcgcgtgcgc ttctgatggc gcccgtagcg gatcgcgaac
    14881 acctcgtaag cttcggggtc tgacatcgtc tcgctcctag aaggcgttca tgacctcgcc
    14941 gatcggctgc cacgacgtgc cgtcgaagcg cgacagctgc agctgctgca gcggatagaa
    15001 gtcgtcggtg ccggtggtga cggtgatgcc cggcagcagc atcggcacct ccaggtggtc
    15061 gaggctcgcc gcctggcgca tgacgttctc gcgcgtcagg tcgtcgccgc actggcgcaa
    15121 cgtcgcctcc agggtcgccg cgacggcgta gccgtaggca ttgaacacct cggccttgtt
    15181 ggcgccggaa ttgtacttgt ccatccaggc gtcccacgtc ttgacgtccg cgtcgttgcc
    15241 ccacttggga tcgctcgggt ccttgaggta gaacgagctg atcagtccga ccgacttgtc
    15301 gagcccggcg ggcttgagca ccgcctcgac cgagttggag acgttgttga gcaggtgcag
    15361 cggccgccag ccgatgtcgt agaccttgcg gatcgcctgg gcggcgaact tgggcgtggt
    15421 gacgttgaag aacacgttgg cgccgctcgc ctgcatctcg acgatctgcg agtctatcgt
    15481 cgggtcggtc acctcgtaag gtgcctcggc aacgatcagc ttgtccgcca gctcgcccag
    15541 gccggcgcgg aacccgtcca cgtagtcctt gccgtagtcg tcgttctggt acagcaccgc
    15601 gatcttcgga tcgtcgacgt tgtcgagcac gtacttggcg taaatcttgg attcgtccga
    15661 atagggcggc tgccagccca tcgtccacgg atactccttc gggttgcccc acttggtcgc
    15721 gccggtggcg atgaagatgt gcggcacctt gcgcgagttg acgtatttgt ggatcgccga
    15781 gttggtcggc gtgcccagcg tctggaacag cagcagcacc tcgtcctgct cgaccaggcg
    15841 gcggatctgc tcgaccgtct tgggcgggct gtagccgtcg tcgtaagaga tgaagttcac
    15901 cttgcggccg ttgatgccgc cgttggcgtt gaccatgtcg aaatagccgc cgatcgcctt
    15961 gccgatggcg ccataggccg aggccggccc gctgtacgga ttggtgttgc cgaccttgat
    16021 ctcggtgtcg ctggcaccgg gcccgtattt cggctccgcg gcgacgctgc cggccgccag
    16081 gccgagcgcg attgccgccg acgccagcct cgccacacac aaccttgtca tctcatcgtc
    16141 ctcccgttct cgttattcgg tcacggcctc gccgccggcc ccggcccggc cggcgcccgc
    16201 ctcgccagcc ggcgcgcgat ccggccgccg gccattgtaa caaagcccgc ggcgcccttc
    16261 ggcatcagat acatcatgcc gatcatgaaa atgccgtaga tcgcccacgg cgcggccttg
    16321 gagacctgct cggccaggtt gggcacgaac acgatgaaca gcccgcccac gaccgagccc
    16381 cagatcgagc cgagcccgcc caccaccatg ccgatgacga acaggatcga gaccaggaag
    16441 ccgaagctgt cggggccgac gaactcgacc gcgatcgcgc cgagcgcgcc ggccagcccg
    16501 gtataggcgg cgctgacgcc gaaggtcagc gacttggtga gcccggtatc gacgcccatc
    16561 gcctcggccg cgatcaggtg cgtgcggatc gccgccatcg cccgccccga gcggccgcgc
    16621 agcaggttgt gggcaagcgc gaaggcgacc acggcgaccg cgagcgtgaa caggtacagc
    16681 cactggtcgg cgctgagcgc gagcccgaac ggcgcctcgg gcttgaagat gaagatgccc
    16741 tggacgccgc cggtccacgg ctcgagcggg tcgtatttga ggatctgcgg caccgagacc
    16801 gcgagcgcga aggtagcgag cgcgagatag tgcccgcgca gccgcccggc cggaaagccg
    16861 aacaggaaac cgaccgccag gcagaccacg aacgcgaccg ggagagtcag gtaatacgcg
    16921 accccggccc ggtccatcag gatcgccgcg gtataggcgc cgagcgcata gaacgcgccg
    16981 tggccgagcg agatctggcc gcagcgcccg gtcagcacgt tcaggcccag gatcgcgatg
    17041 gcgtagatca gcgccagcgt gacctggaag aggcggtagt cgctgagcac gaacgccagc
    17101 gccgcggccg ccgccagcgc cgcggcgagg cccaggcgac gcgcgtttcc ggccccggtc
    17161 atcgcctcac acccgggtca ccgccacccg gccgaacagg ccgccggggc ggaacaccag
    17221 caccgcgacg atgatcgcga gcgcgaccgt gagcttgagc tcgttgccga tgaacggcac
    17281 ataggcgccg accaggttct cgagcacgcc gacgacgaag ccgccggcga ccgcgccacc
    17341 cgggctgttg atgccgccca acagcgcgcc ggcgaagccg tacagcagca ccccgctcat
    17401 catcgacggg tcgaggaaca cgctgtgcgc gaccatcatg ccggcgaccg cgccgatcgc
    17461 ggcggcgagc ccccagccga gcgcgagcat ccagccgacc cggatgccga ccagccggct
    17521 cgaggtcggg ttctgggcgg cggcgcgcat ggcgaggccg aggggggtga agcggaagaa
    17581 caggtacagg agcacgagca ccgcgagcgt tacgccgatc gcgccgacgt cgtgcatgcg
    17641 caacgacacg ccggcgccga gcgcgaacgg ggcctcgggg aacgggctcg ggaagctctt
    17701 gatggtgtag gtccagatcc atcccgccag gctgttgaag atcaccagca ggccgatgaa
    17761 gacgatgatc agggtcagga tcgaggcctt ctcgaacggc ctgatgatca cccgctcgat
    17821 ggccacgccg ccggcgaacg aggccgcgac ggtgatgaag aacgccgccc agaacggcat
    17881 ccccgcctgg atcagggtcc aggcgacgta ggtcgagaac atcgccatct cgccctgggc
    17941 gaaattgatc agctcggtcg cctcatggat catcaccagc gcaagcgcga tcgaggcgta
    18001 gattccgccc gcggcgagac cggctaccac gagctgaatg aacaggtcca tgccagtacg
    18061 ggccctcacg cgtcgcggct ggccgcctca tccttcgaga cgggccttcg gccctcctca
    18121 ggatgaggct ccaaccgatc aagccccggg gcaaacctca tcctgaggag cccgcgcagc
    18181 gggcgtctcg aaggatgagg tttccgaccg aagcggcgaa cggacatcga caccggtctg
    18241 ccccgcgccg cctcagtaac cgagatatgc ccggcggacc gactcgtccg ccttgagcgc
    18301 tgccgacggc ccggccatga ccacccgccc ggtctcgatc agataggcgt cctgggccag
    18361 ctcgagcgcg aggcccgcgt cctgctcgac cagcagcatg ccgatccgct gctcgcggtt
    18421 gatcgtgcgc aggatgtcga acagcccctg gaccaccagc ggcgccaggc cgaacgacgg
    18481 ctcgtcgagc agcatcagcc gcgggcgcag catcagcgca cgcccgatcg ccagcatctg
    18541 ctgctcgccg ccgctcaagg tgccggcctg ctggcggcgg cgctcgccga ggcgcggaaa
    18601 gtaatcatag acgcgctccg catcgcggcg cgcgccggcg cggtcccggc gcgtgaacgc
    18661 gccgagcctc aggttctcct cgaccgtgag cgcggtgaag gtgccgcgcc cgtcgggcac
    18721 gtggccgatg ccgagccgga ccagatcctc ggtggcgcgg ccgtcgatgc gggcgccgtc
    18781 gaaggcgatc tcgccgccgg cacggaccat gccgcacagc gccctgagcg cggtggtctt
    18841 gccggcgccg ttggcgccga ggatggtgga tatgccgccc tcggccacgc cgaagtcgat
    18901 gccgaacagc acctgcaccg ggccgtactg ggcggtcagc ccgcgcgcct cgagcaacgc
    18961 cgccatcagc cgcccgtgcc gagataggcg gtgatcaccg cctcgtcgcg ctgcacctgg
    19021 gcgggcgttc cttcggcgat cttgcgcccg aagtcgagcg ccaccaccca gtccgagatc
    19081 ggcatcacca ggcccatgtg gtgctcgacc aggaggatgg tcagcgcgcg cgcgtcgcgg
    19141 atgtccttga tcagggcgcc gagccgctgc acctcctcgt ggttgagccc gccggcgggc
    19201 tcgtccatca gcagcaatcg tggccgcccc gccagcgcgc gcgcgagctc gacccgcttc
    19261 cgggtgccga aagccagcga ctcgaccggc tcgcttgcca gcgggccgag atcgaggaaa
    19321 tccaggatct ccatcgcctg ggcgcgcaac gccgcctcct cgcgccgcac ccagggcagg
    19381 cgcagcgcgt cgctcaggaa gtcggcgcgg caccggcagt gggcgccgat caggacgttg
    19441 tcgagcaccg acacgccctc gaacaaggcg aggttctgga aggtgcggcc gatgcctaag
    19501 cgggcgattc gatgggcggg ccgcgcgagc aggtcggtgc cggcgaaccg cacttgcccg
    19561 gagtcggggt ggtagagccg gctcaggcag ttgaacaggg tggtcttgcc ggcaccgttg
    19621 ggaccgatca gggcgacgat ctggcccgga tgcaccgcga acgagacctg gtcgagcgcg
    19681 acgatgccgc cgaagcggat cgacagccgc tcgacctcga gcagcggccc gtcttgcgtg
    19741 cgacctcggg ctgcgggcac gcgagacctc gcgcccgtcg cctcggctgc cttcactgcc
    19801 accgccccgt gccccctccc tgaccccggc gcgatcttaa ccggcccgcg gagtcatgat
    19861 ccagcccgca gcgccgctcc ctcggggcgc gcgagcagca ccgcgatccg gcgcgcgccg
    19921 taggtcgagg cgaccaccgc agcggcgaac gcgatcaccc cgacatggac gtcggccgac
    19981 gacagcagct ggccgtccac caccagcacc accagcgcga tcatgaacac gtcgagcatc
    20041 gaccatttcg acaggccggc gagcgccccc gcgagctgcc cgccgcgcgc gccggcatgc
    20101 ggcaccgcga accacagcgc gaggcacgcc accagcttgg caaccggaaa cgcgatggtg
    20161 aatgcgaagg tgatcaggaa caggaaccag tcgccggcgg tggcgaacgc gaacaccgcc
    20221 tcggcaagcg aatactcgcg cgccagcaca aacatcttgc gcaccgtgac cgcgggcagg
    20281 aacaggccgg cgccgagcag ggcggcggcc agcaccagcc atagcccgac cagacggtcg
    20341 tggccggtgg cgtgcgcggc gagcgtaccg gggcgaaagc ggagggcgaa gcgcgcgcgc
    20401 atggcccgag tgtagcagcg tcccggcatc gccgccgcca gcgcgcaacc cacctatgtt
    20461 atgactcgcg ccggcgcatg aggtgcctgc ctgaaccatg tgccgtgcct ccctccgggc
    20521 gtcaacccgg cgacatgccg gctctgaaag gcaattttct atcaatattt tatgccgaat
    20581 tcttcaagca tagactctgc cgttcgtagc gaagccgacg cgacgcccgg cggtgcgccg
    20641 gcggtgaagc gctcggcagg cctgggcgcg gtgcgtcgcg cgctggccca tccgagctac
    20701 cggtcgtaca cggcgggcaa tgcggtgtcg ctgatcggca cctggatcca gcgcatcggg
    20761 gtcggctggc tgacctggca gctgaccgga tccccgacct ggctcggcct gatcgcgttc
    20821 gcggatctgg cgccggcggt ggtcgtcggc ccgctcggcg gcgtgctcgc cgaccgcgtc
    20881 gaccggctgg ccctgatccg gctgatgcaa acctgctcga tgcttcaggc gctggcgttg
    20941 ttcgcgctca ccgcactgga tgcgatctcg atcggcctgc tgttggtgct ggtgctgatc
    21001 aacggcatcg tgatcgggg
//
LOCUS       DASWHU010000121         8553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1909363, whole genome shotgun sequence.
ACCESSION   DASWHU010000121 DASWHU010000000
VERSION     DASWHU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909363"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..453)
                     /locus_tag="VGB88_04300"
     CDS             complement(58..453)
                     /locus_tag="VGB88_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEX9646694.1"
                     /translation="MAIERDIEEIRAAIKAGRFGSEAAVSQGVVLRLLNALGWPAYDT
                     QTVSPQFGLDERRVDYALCHPPAKPIAFIEVKKLGQSDGAERQLFEYAFHKGVQLAIL
                     TDGQEWNFFLPGEQGDYAERRVYKLVDRI"
     gene            complement(738..1436)
                     /locus_tag="VGB88_04305"
     CDS             complement(738..1436)
                     /locus_tag="VGB88_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646695.1"
                     /translation="MLEVRELNAWYGDGHVLQGVSFEVGAGTLTAILGRNGAGKTTLL
                     KAMMGLVRKRSGEVRLDGRRLDRLPAHRIARAGLAYVPETRGIFPSLSVLENLCVAAR
                     PGRGPGAWTLARALESFPRLAERRRAKGGALSGGEQQMLSLARALMTNGRALVLDEPS
                     EGLAPAILDEIEALLAALKADGTTILLVEQNLPLALRLADQVLVLGKGRLRWQGPAAQ
                     FATATEARETWIGV"
     gene            complement(1442..2179)
                     /locus_tag="VGB88_04310"
     CDS             complement(1442..2179)
                     /locus_tag="VGB88_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646696.1"
                     /translation="MLRAEGLHKSFGSLAVARHVDLAVERGARHALIGPNGAGKTTLF
                     NLLSGELRPDAGRIVFGSRDITRDPADARARAGIARSFQRNSLFEEMSARDNLVCAAL
                     LARGLGHVFWKDLRRFTELYEKAEEIASLVGLAESLDRPARSLAYGLQRQLEVGLALA
                     LDPVVLLLDEPTAGMSPEETAAMRVLLAGLPRELTLLVVEHDMDVVFGIADRITVLDA
                     GTVLFEGSPEEVRASPAVRARYLGKRQ"
     gene            complement(2166..3128)
                     /locus_tag="VGB88_04315"
     CDS             complement(2166..3128)
                     /locus_tag="VGB88_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9646697.1"
                     /translation="MTPAAERWRTATALVLAAGAIVAWLTAGIFGHELIAQIAILAIF
                     AMSLDILVGFTGMVSLGHAAFYGIGAYATAALGGVMLDWPPVAAMLGAVAIAALAALV
                     IGAFAVRLGGVFFIMVTLAVGEAIHAYLFKARAFGGDDGLAGIVRPAIAALDLSLDDP
                     RAFSAYALLIAALVYAGLEAVVRSPFGRLLVAIHQNQRRARALGCPVGRVKLAAFTLA
                     GAVAGLAGALTAQHTGFISPELLVWTTSGEVLIVVIVGGMASLVGPAAGAAAVVLASD
                     ALSGATDYWMLFMGLAFIAVVLFADRGLYGVVEGARRRLSRAAR"
     gene            complement(3147..4025)
                     /locus_tag="VGB88_04320"
     CDS             complement(3147..4025)
                     /locus_tag="VGB88_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9646698.1"
                     /translation="MLLDHSFITIQLLNALQLASLLFLLSVGLSVVFGLMNFINLAHG
                     TLYMLGAYVGLSVARATDSFWLALALSPLAVAGVGAALHVGLIGRLQRAAPMTQVLVT
                     FGLIYVGLDAVRYVWGNYSYSIELPAALAGSVEIAGETYPAYRLFLIGLGAAVLAALA
                     LALERTRLGTVVRAGVDDRETAMALGLNVDRAFFIAFCLGCGLAGLAGVAAAPVLSIY
                     PGMDMSILILTLIVVVIGGPGSLAGAALGSLLVGMIETFGQALAPEYASVVLYAAMAA
                     ILLLRPGGLAPARRPA"
     gene            complement(4061..5239)
                     /locus_tag="VGB88_04325"
     CDS             complement(4061..5239)
                     /locus_tag="VGB88_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008281970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646699.1"
                     /translation="MGKAATRLGIACAAALAAGTACAAEPIRIGVILPYSGVYASLGG
                     EITQGLELGFATFGDSFGGRAIELITEDSEVKPNVGLAKAKKLVFEDRVDLLVGPVAS
                     NVANALRDFVHAQQVPLVIPNAGNNLLTGERCSPWLIRTSFSNAQITRGMGAWMVQHG
                     YDTVYLAAADYAAGQQMMDAFRETFTAAGGTVVGESYAPLRETKDFGPFLAQIKAAAP
                     KAVFAFFAGGQAIQFVKQYDAFGLKDSIKLAGAGWLTSPLYIDAEGPAAAGVLGILNY
                     VPGIDTPENAAFQEAYQARYGRVASEFAVQGYDTAHLIAAALEATGGDTSDKAALMAA
                     MRATKFAGPRGPIEIDPATNNVVQDIHIFETVAGANGVELRVIESLPAMRDPANGCKL
                     "
     gene            complement(5420..5956)
                     /locus_tag="VGB88_04330"
     CDS             complement(5420..5956)
                     /locus_tag="VGB88_04330"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9646700.1"
                     /translation="MTAQTEISRSCTNLDAAAFAETKAAVAGDPGLGRGSFQTVTEWT
                     DGAVAVTRARSFTLATDEPRPLGGSDSAIDPMELLLASLGTCLTIGWVTHANQRGIDF
                     EDLKIVVEAPYDLRGYLQLDAGVRPGFLDLSYRVEVKSDADPTVLEEIKRAAEAGSPM
                     FDNIRNPTPIAGRIARIA"
     gene            6188..6646
                     /locus_tag="VGB88_04335"
     CDS             6188..6646
                     /locus_tag="VGB88_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9646701.1"
                     /translation="MDAIDRAILGCLQQDAAMPLAQIAARVGLSSTPCWRRIQKLEEQ
                     GVIRARVTLLDAAKVNLGVTVFVSVRTNQHTAEWLARFKEAVVDFPEVVEAYRMSGEV
                     DYLLRVVVPSIDAYDAFYKRLIAKVPLTDVSSSFAMEQMKFTTALPLDYA"
     gene            6725..7432
                     /locus_tag="VGB88_04340"
     CDS             6725..7432
                     /locus_tag="VGB88_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646702.1"
                     /translation="MESTPIMTLFRTGRGPDWPTYLGLLQRMDREWRSSRVSSQPFGV
                     FGPRPSWQDTIARAQYLFEADDWMDLDQDQCSLIAPAEFYAHGDALLGSIGRRSTAVR
                     NFLFDPDHAQDRDTVLERLKYVQRSSGAQVAARAGTILAELRSLEGMGTAFASRLLAL
                     ARPDWFVVVNNKSRKWLADATGLRLTGKTSYQNLIAWVADQAWHASPEPNDPWERSIW
                     GLRAALLDAFAYHPARG"
     gene            complement(7605..8393)
                     /locus_tag="VGB88_04345"
     CDS             complement(7605..8393)
                     /locus_tag="VGB88_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9646703.1"
                     /translation="MAELRNPVKVRLEAGEIAIGVGLRQARTVDIGKIMRTCGYDWVF
                     IDMEHNSMSIDDAAQIAVAAQDAGIAPFVRVPGTAHHHATRALDGGALGVVFPHVDTP
                     EQARELARNCRYPPRGQRSITGGLPQLGFATLPMAETVAAIDAATMVVVMLESPQAID
                     NADAIAAADGVDALLVGTNDLCLEMGMPGKIDAPEVAVAFETVIAACKKHGKAAALGG
                     VYQPDLMRRYLAMGFRMVLAGSDLSFLMSAAKERAGFLRGLNVG"
BASE COUNT         1366 a         2946 c         2961 g         1280 t
ORIGIN      
        1 atctggactg ccgatcccgg caagatcatc gccgcagtcc gcagagggcg ccaagtgtta
       61 gattcgatcc actagtttgt agacgcgacg ctcggcgtaa tcaccctgtt cgccaggaag
      121 gaagaagttc cattcctgac catcggtcaa aattgcgagc tgtacgcctt tgtgaaaggc
      181 atattcgaac aactgccgtt cggccccgtc actctgaccg agtttcttca cctcgatgaa
      241 agcgatcggc ttggccggcg gatggcacag cgcatagtcg acacgtcttt cgtcgaggcc
      301 gaattgcgga ctgactgttt gcgtgtcgta tgcgggccag cccagtgcgt ttagcaagcg
      361 gagcacgacg ccctgggaga cggccgcctc acttccaaag cggccagctt tgatggccgc
      421 gcgaatttct tcaatatctc gctctatcgc cattcccgga ttccccacat aggtacagca
      481 ccaccctctg cgtcacccgg ctcgccgcct cacgaagcgc agtataacat tttccgcgca
      541 gtaagcacgg cggcgcctgc tcgaatagcg cttttgttgt ctccaggaat tggcgccggg
      601 tcccgagccc ctgggaaatc tggttgggag aaattggcgt tcgagcagaa tactgcgatg
      661 aagtcgaaaa ccagtgtcag ccatgtgccg gcaccgggga gcaggcacgg ccgttatgcc
      721 acaaggcgca tatcgcccta caccccgatc caggtctcgc gcgcctcagt cgcggtggcg
      781 aactgggcgg cggggccttg ccagcgcagc cggcccttac cgagcacgag cacctggtcg
      841 gcgaggcgga gcgcgagcgg caggttctgc tcaacgagca ggatcgtggt gccgtcggcc
      901 ttgagcgcgg cgagcagggc ctcgatctcg tcgaggatcg cgggcgccag gccctccgac
      961 ggctcgtcga gcacgagtgc ccggccgttg gtcatcagcg cacgcgcgag gctgagcatc
     1021 tgctgctcgc cgcccgagag cgcaccgccc ttcgcgcgcc ggcgctcggc gaggcgcggg
     1081 aagctttcga gcgcgcgcgc caaagtccac gcccccggcc cgcgcccggg cctggccgcc
     1141 acgcacaaat tctcgagcac gctgagcgag gggaagatgc cgcgcgtctc gggcacgtag
     1201 gcgaggcctg cacgtgcgat ccggtgcgcg ggcagccggt cgagccggcg cccgtcgagg
     1261 cgcacctcgc cgctgcgctt ccgcaccagg cccatcatcg ccttcaggag cgtcgtcttg
     1321 ccggcgccgt tgcgcccgag gatggcggtg agcgtgcccg caccgacctc aaacgacact
     1381 ccctggagca cgtgcccgtc gccgtaccag gcattgagct cgcgcacctc gagcagcgcc
     1441 gtcattgccg cttgcccaga tagcgcgcgc gcacggcggg tgaggcgcgc acctcctcag
     1501 ggctgccctc gaacagcacc gtgccggcat cgagcacggt gatgcggtcg gcgatgccga
     1561 acaccacgtc catgtcgtgc tcgaccacca gcagggtgag ctcgcgcggc aggcccgcca
     1621 gcagcacgcg catcgccgcg gtctcctctg ggctcatgcc cgcggtcggc tcgtcgagca
     1681 agaggacaac cggatcgagc gccagcgcca agcccacctc gagctggcgc tggaggccat
     1741 aggcgaggct ccgcgccggc cggtccaggc tctcggcgag gccgaccaaa gacgctattt
     1801 cttccgcttt ttcatagagt tctgtaaatc gtctcaagtc cttccagaac acgtggccga
     1861 ggccgcgcgc gagcagcgcc gcgcacacca ggttgtcgcg cgcgctcatc tcctcgaaca
     1921 ggctgttgcg ctggaacgaa cgcgcgatgc cggcgcgggc gcgcgcgtcc gcggggtcgc
     1981 gggtgatgtc gcgcgagccg aacacgatgc gcccggcgtc gggcctgagc tcgcccgaga
     2041 gcaggttgaa caaggtcgtc ttgccggcgc cgttggggcc gatcagcgcg tggcgcgcgc
     2101 cgcgctcgac cgcgaggtcg acatggcggg cgaccgcgag cgagccgaag ctcttatgca
     2161 ggccttcagc gcgcagcacg gctcaacctc cggcgcgcgc cctcgaccac gccgtacagg
     2221 ccccggtccg cgaacagcac cacggcgatg aacgcgaggc ccatgaacag catccagtag
     2281 tcggtggcgc cgctcagcgc gtcgctcgcc agtaccaccg cggcggcgcc ggcggcgggc
     2341 ccgaccaggc tcgccatgcc gccgacgatg accacgatca gcacctcgcc cgaggtcgtc
     2401 cacaccaaga gctccggcga gatgaagccg gtgtgctggg cggtgagcgc gccggcgagc
     2461 ccggccacgg cgccggcgag ggtgaacgcg gcgagcttga cccggcccac cgggcagccg
     2521 agagcgcgcg cccgccgctg gttctggtgg atcgcgacca ggagccgtcc gaacggcgag
     2581 cgcaccaccg cctcgagccc ggcatagacc agggccgcga tcagcagcgc ataagccgag
     2641 aacgcgcgcg ggtcgtcgag cgagagatcg agcgcggcaa tcgcggggcg cacgatgccg
     2701 gccagcccgt cgtcgccgcc gaaggcgcgc gccttgaaca ggtaggcgtg gatcgcctcg
     2761 ccgaccgcga gcgtgaccat gatgaagaac acgccgccga gccggaccgc gaacgccccg
     2821 atcaccagcg ccgcaagcgc cgcgatcgcg accgcgccca acatcgcggc gaccggcggc
     2881 cagtcgagca tcaccccgcc gagcgccgcg gtggcgtagg cgccgatgcc gtagaacgcg
     2941 gcatggccga gcgagaccat gcccgtaaac ccgaccagga tgtcgaggct catggcgaag
     3001 atcgccagga tcgcgatctg ggcgatcagc tcgtggccga agattccggc cgtgagccag
     3061 gcaacgatcg ccccggcggc gagcaccagg gccgtcgccg tgcgccagcg ctcggcggca
     3121 ggagtcacgg cggcggcggc gcggcctcag gccggccggc gcgcgggcgc cagcccgccc
     3181 gggcgcagca gcaggatcgc cgccatcgcc gcgtacagca cgacgctcgc atattccggc
     3241 gccagcgcct ggccgaaggt ctcgatcatg ccgaccagga gcgagccgag cgcggcgccg
     3301 gcgaggctcc ccgggccgcc gatcacgacc actatcaggg tcaggatcag gatcgacatg
     3361 tccatgcccg gatagatcga cagcaccggc gccgccgcca cgccggcgag cccggcgagc
     3421 ccgcagccga ggcagaaggc gatgaagaag gcgcggtcga cgttaagccc gagcgccatc
     3481 gcggtctcgc ggtcgtcgac gcccgcgcgc accacggtgc cgagccgggt gcgctcgagc
     3541 gcgagcgcga gcgccgccag caccgccgcg ccgagcccga tcaggaacag ccgataggcg
     3601 ggataggtct cgccggcgat ctcgacgctg cccgcgagcg ccgccggcag ctcgatcgaa
     3661 tagctgtagt tgccccacac gtagcgcacc gcatccagcc cgacatagat caggccgaag
     3721 gtgaccagca cctgggtcat cggcgcggcg cgctgcagcc ggccgatcag cccgacatgg
     3781 agggccgcgc cgacgcccgc caccgccagg gggctgagcg cgagcgcgag ccagaacgag
     3841 tcggtcgcgc gcgccacgct gaggccgaca taggcaccca gcatgtacag cgtgccgtgc
     3901 gccaggttga tgaagttcat cagcccgaac accaccgaca gcccgaccga gagcaggaac
     3961 aataggctcg cgagctggag tgcattgagc agctggatgg tgatgaagct gtggtcgagc
     4021 agcaccgcaa cgcgccttcg cggggccggt gccggagcgg ctacagcttg cagccgttgg
     4081 ccggatcgcg catcgccggc aggctctcga tcacccgcag ctcgacgccg ttggccccgg
     4141 ccacggtctc gaagatgtgg atgtcctgga cgacgttgtt ggtcgcgggg tcgatctcga
     4201 tcgggccgcg cgggccggcg aacttggtcg cgcgcatcgc cgccatcagc gccgccttgt
     4261 cggaggtgtc gccgccggtg gcctcaagcg ccgccgcgat caggtgcgcg gtgtcgtagc
     4321 cctggaccgc gaactcggag gcgacgcggc cgtagcgcgc ctgataagcc tcttggaacg
     4381 ccgcgttctc gggcgtgtcg atgccgggca cgtagttgag gatgccgagc accccggccg
     4441 cggccgggcc ttcggcgtcg atgtacagcg gcgaggtcag ccagccggcg ccggcgagct
     4501 tgatcgagtc cttgagcccg aaggcgtcgt actgcttgac gaactggatc gcctggccgc
     4561 cggcgaagaa cgcgaacacc gccttcggcg ccgccgcctt gatctgggcc aggaacgggc
     4621 cgaagtcctt ggtctcgcgc agcggcgcgt aggactcgcc gaccaccgtg ccgccggcgg
     4681 cggtgaaggt ctcgcggaag gcgtccatca tctgctggcc ggcggcgtag tcggcggcgg
     4741 cgagataaac cgtatcgtag ccgtgctgca ccatccacgc gcccatgccg cgggtgatct
     4801 gggcgttcga gaacgaggtc cggatcagcc acgggctgca gcgctcgccg gtcaaaaggt
     4861 tgttgcccgc gttcgggatc accagcggga cctgctgggc gtggacgaag tcgcgcagcg
     4921 cgttggcgac gttcgaggcc accggcccga ccagcaggtc gacccggtcc tcgaacacca
     4981 gcttcttggc cttggcgagg ccgacgttgg gcttgacctc ggagtcctcg gtgatcagct
     5041 cgatcgcgcg cccgccaaag ctgtcgccga aggtggcgaa gccgagctcg aggccctggg
     5101 tgatctcgcc gccgaggctg gcatagacgc ccgaataggg caggatcacg ccgatccgga
     5161 tcggctccgc cgcgcacgcc gtgcccgccg ccagcgcggc cgcgcaggcg atgccgagcc
     5221 gtgttgcggc cttccccatg tcgcgcctcc ccgtccgctc gccccggtcc tctggccggg
     5281 gccggcacta tagcaaggcg cggacggcgg caatagcggg gccccgaggc ggcggtgctg
     5341 gtctgccccc ggggcccgca gtggagggga ggtgctcggc gtgcgccgac gggccccggg
     5401 ggagtggcgg tgcggcggct caggcgatgc gcgcgatgcg accggcgatc ggggtcgggt
     5461 tgcggatgtt gtcgaacatc ggcgagccgg cctcggcggc gcgcttgatc tcctcgagca
     5521 ccgtcgggtc cgcgtccgac ttgacctcga cccggtagct gaggtcgagg aagcccgggc
     5581 gcacgcccgc atcgagctgc aggtatccgc gcaggtcgta gggcgcctcg accacgatct
     5641 tgaggtcctc gaagtcgatg ccgcgctggt tggcgtgggt cacccagccg atggtgaggc
     5701 aggtgccgag cgaggcgagc agcagctcca tcgggtcgat cgccgagtcg ctgccgccga
     5761 gcggcctggg ctcgtcggtc gccagcgtga acgagcgcgc gcgcgtgacc gcgaccgcgc
     5821 cgtcggtcca ttcggtgacg gtctggaagc tgccccggcc gaggcccggg tcgccggcca
     5881 ccgcggcctt ggtctcggcg aacgcggcgg cgtcgaggtt ggtgcaggaa cggctgatct
     5941 cggtctgtgc ggtcatggcg gctgctctcg cctcgctgtt gtttgtcctc ttgcgcgccc
     6001 ggacgcgtgc cggccggccc cgccgtgcgc atgctttgct gttatcagcg ccagccgcgc
     6061 cggacaatgt cggcgaatag cccgcttgga taaaattgtt ctacaaattg cctcgtatga
     6121 gaaaaatttg ctaaatatag ctagtcaatg acatatttgt aggacgtaat tccatttcga
     6181 gggacgcatg gacgcgatcg accgggccat tctcggctgc ttgcagcagg acgcggcgat
     6241 gccgctggcg cagatcgcgg cgcgcgtcgg gctgtcgtcg acgccgtgct ggcggcgcat
     6301 ccagaagctc gaggaacagg gcgtgatccg cgcgcgcgtc acgctgctcg acgccgccaa
     6361 ggtcaacctc ggcgtgaccg tgttcgtctc ggtgcgcacc aaccagcaca ccgccgagtg
     6421 gctggccagg ttcaaggagg cggtggtcga ctttcccgag gtggtcgagg cctaccggat
     6481 gagcggcgag gtcgactacc tgctgcgcgt ggtggtgccc agcatcgacg cctacgacgc
     6541 cttctacaag cgcctgatcg ccaaggtgcc gctcaccgac gtgagctcga gcttcgccat
     6601 ggagcaaatg aagttcacca ccgccctgcc gctggactac gcgtgacata ttgccgagtc
     6661 ggcgaaactc ggtgtctgct acgatcttgc tctgccggtg taatcttcgc catggagcgc
     6721 gcgcatggaa tcgacgccga tcatgacgct gtttcgcacc ggtcgcggcc ccgactggcc
     6781 gacttatctc ggcttgctcc agcgaatgga ccgcgaatgg cggtcgagcc gcgtatcctc
     6841 gcagccattc ggcgtgttcg ggccgcggcc aagctggcag gacaccatcg cccgcgccca
     6901 gtacttgttc gaggccgacg actggatgga cctcgaccag gaccagtgtt cgctcatcgc
     6961 acccgcagaa ttctacgcgc acggggacgc tctgctcggc agcattggcc ggcgcagcac
     7021 ggccgtcagg aactttctgt tcgaccccga tcatgcacag gaccgggaca cggttctcga
     7081 acgactgaaa tatgtgcagc ggtcctccgg cgcgcaggtc gcggcgcgcg cggggacgat
     7141 tctcgcggaa ctgcgctcgc tggagggcat gggaactgcg tttgcgagcc gactgcttgc
     7201 gcttgcgcgg cccgactggt tcgtcgtcgt gaacaacaaa tcgcgcaaat ggctcgcgga
     7261 tgcgacaggg ctgcgcctga ccggaaagac aagctatcag aacctgatcg catgggtggc
     7321 ggaccaggct tggcatgcga gtcccgagcc gaacgatccg tgggagcggt cgatctgggg
     7381 cttgcgcgcc gcgttactcg acgccttcgc ctatcatccg gcccgtggtt agcgtgcggt
     7441 acagccccac tttgccgtca tcgcgagccg ccgcaggcgg cgtggcgatc ccgaaaccag
     7501 ccggacgcgc tgcccgaggt gctggattgc ttcgcccgcg atgcgggctc gcaacgacct
     7561 cgttaccgct gcgccgtttt tgacgtcgac atgcggcggc cggctcaacc gacgttgagg
     7621 ccgcgcagga agccggcgcg ctctttcgcg gcggacatca ggaaggagag gtcggagccg
     7681 gcgagcacca tgcggaagcc catggcgagg tagcggcgca tgagatccgg ctggtagacg
     7741 ccgccgagcg cggccgcctt gccgtgtttc ttgcacgcgg cgatcaccgt ctcgaacgcg
     7801 accgcgacct cgggcgcgtc gatcttgccc ggcatgccca tctcgaggca caggtcgttg
     7861 gtgccgacca agagcgcgtc gaccccgtcg gcggcggcga tcgcgtccgc gttgtcgatc
     7921 gcttgcgggg actcgagcat caccaccacc atggtcgcgg cgtcgatcgc ggcgacggtc
     7981 tcggccatcg gcagggtggc gaagccgagc tgcggcaatc caccggtgat cgagcgctgg
     8041 ccgcgcggcg ggtagcggca gttgcgcgcc agctcgcgcg cctgctcggg ggtgtcgaca
     8101 tgcgggaaca ccaccccgag cgcgccgccg tcgagcgcgc gcgtcgcgtg gtggtgggcg
     8161 gtgcccggca cgcgcacgaa cggcgctatg ccggcatcct gggcggcgac cgcgatctgc
     8221 gccgcgtcgt cgatcgacat cgagttgtgc tccatgtcga tgaacaccca gtcgtagccg
     8281 caggtgcgca tgatcttgcc gatgtcgacc gtgcgcgcct gcctgaggcc gacgccgatg
     8341 gcgatctcgc ccgcctcgag cctcaccttc accgggttgc gcagctctgc catcttcccc
     8401 ctccctggtt cgcgccagag cttgcaccgg ctccgccctt gcccgcaagg cgctttcgcc
     8461 taaactctgc cggccgcggc ggaagggaca ccatgtacga cctcttgatc aaggacgcgc
     8521 gcatccatga cgggctcggg gccgagccgg tcg
//
LOCUS       DASWHU010000122         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1532401, whole genome shotgun sequence.
ACCESSION   DASWHU010000122 DASWHU010000000
VERSION     DASWHU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532401"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGB88_04350"
     CDS             <1..466
                     /locus_tag="VGB88_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9646704.1"
                     /translation="FAAIGNDRQFARFTAALGAPALAEDARFRTNADRVINRDALRDA
                     LAPLLADKDGVALSEHLLGEGVPAGPVLEIPEVLDHAQVRARDMVVERGAYRGLGTPI
                     KMSRTPGDPRTPPPRLSEHARTVLANAGYSETEIDSLIDHGTVAGARERATP"
     gene            complement(562..954)
                     /locus_tag="VGB88_04355"
     CDS             complement(562..954)
                     /locus_tag="VGB88_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9646705.1"
                     /translation="MIVVDASAALEALLRTPAAAAVEERLFDSGETLHAPHLIDVEVA
                     QVLRRYANAGEVDAERCRAALADLADFPLNRYPHDVLLLRVWDLRANLTAYDAVYVAL
                     AEALDAPLLTRDRRLAAAPGHRARIELV"
     gene            complement(951..1181)
                     /locus_tag="VGB88_04360"
     CDS             complement(951..1181)
                     /locus_tag="VGB88_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646706.1"
                     /translation="MSRMIQIRNVPDALHHRLKARAAMAGMSLSDYLLDEIRRAAERP
                     TPDELRARLAGRAAAAPAISPAEAVRAERDRG"
     gene            1284..1523
                     /locus_tag="VGB88_04365"
     CDS             1284..1523
                     /locus_tag="VGB88_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9646707.1"
                     /translation="MDLKLMDLARITARGRMTIPKRVREAAGLHEGDVIAFEIERGHL
                     VVRKVAAGRDDDLGGLSRTVSEWASPEDEAAWRDL"
     gene            complement(1531..2697)
                     /locus_tag="VGB88_04370"
     CDS             complement(1531..2697)
                     /locus_tag="VGB88_04370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9646708.1"
                     /translation="MDFSLTPEQDQIVATVARICARFDDGYWLACDREARFPHDFAAA
                     LAADGWLGIAMPQAYGGAGLGVTEAALMLQTIAASGAAMSGCSAVHMNIFGPNPIVVF
                     GTDDQKRRFLPPLIAGKERACFAVTEPDAGLDTTRIRTRARRDGDGYVIDGRKVWTST
                     AQESDKVMLLARTTALEDCARPTEGMTLFYADLDRSAVDVRRIAKMGRHAVDSNELFI
                     DGLRVPAGDRIGEEGDGFRCLLHGLNPERILVGAEAVGIGRDALDRAARYARERIVFD
                     RPIGQNQAIQHPLAESWAELEAANLMVLKAAALYDQGRACGIEANAAKLLGARAGYRA
                     CERAVLTHGGYGYAAEFHVERLMREVWIDRLAPVSEQLILCHIAEKALGLPKSY"
     gene            2818..>3299
                     /locus_tag="VGB88_04375"
     CDS             2818..>3299
                     /locus_tag="VGB88_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX9646709.1"
                     /translation="MIAYPAAVRALCVAALATWGSAAAAGDDWTGDLSPIGAADWSYD
                     RARHLLERAGFGGTPGEIAALAAMTPEEAVRRLVDYEALDDGALPAFDESDIFDPGLV
                     DFPPSRPATTALAKETGEALGVKVKPAGNRPLQPVVDKFFYWLRASMLETRRVSYWWA
                     "
BASE COUNT          483 a         1147 c         1208 g          461 t
ORIGIN      
        1 gttcgcggcg atcggcaacg accgccagtt cgcgcgcttc acggccgcgc tcggcgcgcc
       61 cgcactcgcc gaggacgcgc gcttccgcac caacgccgac cgggtgatca accgcgacgc
      121 gctgcgcgac gccctcgcgc cactgcttgc ggacaaggat ggcgttgcgc tgtcggagca
      181 cctgctgggc gagggcgttc ccgcgggccc ggtgctcgag attcccgagg tcctcgatca
      241 cgcgcaggtg cgagcccgcg acatggtggt cgagcgcggc gcgtatcgcg gcctcggcac
      301 gccgatcaag atgagccgca cgccgggcga cccgcgcacc ccgccgccgc gcctgtccga
      361 gcacgcacgc acggtgctcg ccaatgccgg ctactccgag accgagatcg acagcctgat
      421 cgaccacggc accgtcgcgg gcgcgcgcga gcgcgcgacg ccgtaagccg cctgatccgg
      481 accccgagcc gacccggaac tcggcggcat ggccgcgccg gtcgtgtctt gaaagccgtt
      541 gaaggggccg tttcaacgcg cttaaacgag ctcgatgcgc gcgcggtgcc cgggcgcggc
      601 ggcgagccgc cggtcgcggg tgagcaaagg cgcgtccagt gcttccgcga gcgccacgta
      661 gaccgcgtcg taagccgtca ggttggcgcg caggtcccac acgcgcagca gcagaacgtc
      721 gtgcggataa cggttcaagg gaaagtcggc gaggtcggcg agggccgcgc ggcagcgttc
      781 cgcatcgacc tcgccggcgt tcgcgtatcg gcgcagcacc tgcgccacct cgacgtcgat
      841 caggtgcggt gcgtgaagcg tctcaccgga atcgaacagc cgctcttcca ctgccgctgc
      901 cgcaggcgtc cgcaggagcg cctcgagcgc cgccgaggcg tcgacgacga tcacccgcgg
      961 tcgcgctcgg cgcgcaccgc ctcggccggc gagatggcgg gcgccgccgc ggcgcggccg
     1021 gccaggcgcg cgcgcagctc gtccggcgtc ggccgctcgg ccgcgcggcg gatctcgtcg
     1081 agcaggtagt cggacagcga catgcccgcc atggccgcgc gcgccttgag ccggtgatgc
     1141 agcgcgtcgg ggacgttgcg gatctggatc atgcgcgaca tgtcataagc atgctcgcat
     1201 gtgttgagca tgtcaattga cccgacaaac ccatgccctt tcatggcaac ttgcggtagc
     1261 tcagtgtcgg atgactcaga agcgtggacc ttaagctcat ggaccttgcc aggatcacgg
     1321 cgcgcggccg gatgacgatc ccgaagcgcg tccgcgaggc ggccggccta cacgagggcg
     1381 acgtgattgc cttcgagatc gaacgcggcc atttggtcgt gcgcaaggtc gccgcgggcc
     1441 gcgacgacga cctcgggggg ctgtcgcgca ccgtgagcga atgggcgtcg ccggaggacg
     1501 aggccgcctg gcgcgacctg tgagccgcgc tcagtacgac ttgggcaggc cgagcgcctt
     1561 ttcggcgatg tggcacagga tcagctgctc actgaccggc gccaggcggt cgatccagac
     1621 ctcgcgcatc agccgctcga cgtggaactc ggcggcgtag ccgtagccgc cgtgggtcag
     1681 caccgcgcgc tcgcaggcgc ggtagccggc gcgcgcgccg agcagcttgg cggcattggc
     1741 ctcgatgccg caggcgcgcc cctggtcgta gagcgcggcc gccttgagca ccatcaggtt
     1801 cgccgcctcg agctcggccc aggactcggc gagcggatgc tggattgcct ggttctggcc
     1861 gatcgggcgg tcgaacacga tgcgctcgcg cgcatacctc gccgcgcgat cgagagcgtc
     1921 gcggccgatg ccgaccgcct cggcgccgac caggatgcgc tcggggttga ggccgtgcag
     1981 gaggcagcgg aagccatcgc cctcctcccc gatgcggtcg cccgccggca cgcgcaagcc
     2041 gtcgatgaac agctcgttcg aatcgaccgc atggcggccc atcttggcga tccggcgcac
     2101 gtccacggcg ctgcggtcga ggtcggcgta gaacagggtc atgccctcgg tcgggcgcgc
     2161 gcagtcctcg agcgcggtgg tgcgcgcgag cagcatcacc ttgtcggatt cttgcgcggt
     2221 cgaggtccac accttgcgcc cgtcgatcac gtaaccgtcg ccgtcgcggc gggcgcgggt
     2281 gcggatgcgg gtggtgtcga ggccggcgtc gggctcggtg accgcgaagc aggcgcgctc
     2341 cttgcccgca atcagcggcg gcaggaagcg gcgcttctgg tcgtcggtgc cgaacacgac
     2401 gatcgggttg gggccgaaga tgttcatgtg caccgccgag cagccgctca tcgccgcgcc
     2461 cgaggcggcg atcgtctgca gcatcagcgc cgcctcggtg accccgagcc cggcgccgcc
     2521 ataggcctgg ggcatggcaa tgccgagcca gccgtcggcg gcgagcgcgg ccgcgaagtc
     2581 gtgcggaaag cgcgcctcgc ggtcgcaggc gagccagtag ccgtcgtcga agcgggcgca
     2641 gatgcgcgcg accgtggcga cgatttggtc ctgttcggga gtgaggctaa aatccatctt
     2701 gcgcccccgc gcccggttcg agcgatcgcc caggcgaata gcacggattg gcccggcgaa
     2761 taagggcggg tgatagaatt gccgatcgag cacgtccgtc ggtaagggag agacccgatg
     2821 atcgcatatc cggccgcagt tcgggcgctg tgcgtcgccg cgctggcgac ctgggggtcg
     2881 gcggcggcgg ccggcgacga ctggaccggc gacctgagcc cgatcggtgc cgccgactgg
     2941 agttacgacc gcgcccgcca cctgctcgag cgcgccggct tcggcggcac cccgggcgag
     3001 atcgcggcgc tggcggcgat gaccccggag gaggcggtgc gccggctggt cgactacgag
     3061 gcgctcgacg acggcgccct gccggcattc gacgagtcgg acatcttcga tccggggctg
     3121 gtcgacttcc cgccgagccg ccccgccact acggctctcg ccaaggagac cggcgaggcg
     3181 ctgggggtca aggtcaagcc cgccggcaac cggccgctgc agccggtggt cgacaagttc
     3241 ttctactggc tgcgcgcgag catgctcgag acccgccggg tgtcctactg gtgggcgaa
//
LOCUS       DASWHU010000123         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1532523, whole genome shotgun sequence.
ACCESSION   DASWHU010000123 DASWHU010000000
VERSION     DASWHU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532523"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1404)
                     /locus_tag="VGB88_04380"
     CDS             complement(325..1404)
                     /locus_tag="VGB88_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9646710.1"
                     /translation="MRRRRFLTGAAAGVAAAAAGARAAPAIAQSRIEWRMVTTWPRNF
                     PAIGTGAQRIADSITELSDGRLKVTLYGAGELVPAFEVFDYVREGNAECGHDAPYYWI
                     AKHSATPFFCSVPGGLAPFEHMAWIYSGGGQALWDELYAPFGLRAFLAGTGGPNMGGW
                     FRKEINSVGDLKGLKMRIPGLGGAMLNRLGVTTVNMSGGEILPALQTGALDASEWSGP
                     YLDLAIGFHKVVKYYYGPGMHEPGPAFSLLVNIAAYDALPADLKAIVRAAAAEENGRM
                     MAELTAFNASALLTLVKEHGVEVRHFPPDVITAMFEAAAEVIGETAAATEIGGRIYAG
                     WRAFRETMIANGPYSELGFMLMREA"
     gene            complement(1451..1762)
                     /locus_tag="VGB88_04385"
     CDS             complement(1451..1762)
                     /locus_tag="VGB88_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEX9646711.1"
                     /translation="MATVLFVVKATITPDQEAAFNRWYDTEHCADLLQYRGAVSAKRY
                     KAIMGEDLFQYMAVYEFDSEKTFQAFLKSDHLEKLKADYDAHFGQVSDRARFAYVQVY
                     P"
     gene            1974..>2443
                     /locus_tag="VGB88_04390"
     CDS             1974..>2443
                     /locus_tag="VGB88_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646712.1"
                     /translation="MALGLLSGLAVLAAAGGDARAEPTAHAVAGYLYAVGDAETVHEV
                     GELALRRSSMVAAFAADDPSSALHLATFTCSFVSHTTTDASVRHQDRVGAAGACLVTD
                     RDGDTHVAEWQRPPGAELGTWTVVRGTGKWREATGTGTYDILFLSPPPKPQLRFT"
BASE COUNT          368 a          861 c          842 g          372 t
ORIGIN      
        1 accccgtcgc aatacgcccg caccgagccc tatgcgagct tcctgagggt gctgcgcgag
       61 tccggccggc agatcaagct gccggcgatc gagccgctgc cgccgctgct gaaggcgctc
      121 tagcaggctg atgatttgaa acatggtcat ccatcacccg tcaccccggc caaggcgcgc
      181 agcgccgcga gccggggtcc atcccgccgc ttgcccgggt ggtcgagcgc gtggcaaagg
      241 cgtatgggcc ccggatatcc gcttcgcgga gtccggggtg acgaccaaaa caggctcagg
      301 gatccgactt aatatcatcc cgctctaagc ctcgcgcatg agcatgaagc cgagctcgct
      361 gtacgggccg ttggcgatca tggtctcgcg gaaggcacgc cagccggcgt agatgcgccc
      421 gccgatctcg gtcgccgcgg cggtctcccc gatcacctcg gcggcggcct cgaacatcgc
      481 cgtgatcacg tcgggcggga agtggcgaac ctcgacgccg tgctccttga ccagggtcag
      541 cagggcgctg gcgttgaagg cggtcagctc cgccatcatg cggccgttct cttccgccgc
      601 cgccgcgcgc acgatcgcct tcaggtccgc cggcagcgca tcgtaggcgg cgatgttgac
      661 caggagggag aacgccgggc cgggctcgtg catgcccggg ccgtagtagt acttgaccac
      721 cttgtggaag ccgatggcga ggtcgaggta aggtcccgac cactcgctgg cgtcgagcgc
      781 gccggtctgc agcgccggca gtatctcgcc ccccgacata ttgaccgtgg tcacgccgag
      841 ccggttgagc atcgccccgc cgagcccggg gatgcgcatc ttgagtccct tcaggtcgcc
      901 gaccgagttg atctccttgc ggaaccagcc gcccatgttc ggcccgccgg tgccggcgag
      961 gaacgcgcgc agcccgaacg gtgcgtagag ctcgtcccac agcgcctggc cgccgccgct
     1021 gtagatccac gccatgtgct cgaatggcgc gagcccgccc ggaaccgagc agaagaacgg
     1081 cgtcgccgaa tgcttggcga tccagtaata gggcgcgtcg tggccgcact cggcgttgcc
     1141 ctcgcgcacg tagtcgaaca cctcgaaggc gggcaccagc tcgccggcgc cgtagagggt
     1201 gaccttgagc cggccgtcgg acagctcggt gatcgaatcc gcgatccgct gcgcgccggt
     1261 gccgatggcg gggaagttcc gcggccaggt ggtgaccatc cgccactcga tgcgcgactg
     1321 cgcgatcgcc ggcgcggcac gcgccccggc ggcggccgcg gcgacgccgg ccgccgcgcc
     1381 cgtgaggaat cggcgacgtc tcatactgcc ctccccggtt cgcccggcgg gcgcctctcg
     1441 cgccgccgcg ctacggatag acctggacgt aggcgaagcg ggcgcggtcg gacacctggc
     1501 cgaaatgggc gtcgtagtcg gccttgagct tttccaagtg gtccgacttg aggaacgcct
     1561 ggaacgtctt ctcgctgtcg aactcgtaga ccgccatgta ctggaacagg tcctcgccca
     1621 tgatcgcctt gtagcgcttg gcgctgaccg cgccgcgata ttgcagcagg tcggcgcagt
     1681 gctcggtgtc gtaccagcgg ttgaacgccg cctcctggtc cggcgtgatg gtcgccttga
     1741 cgacgaacag gaccgtggcc atgcgcattc ctccctcgtt gcagcccgcg caccataccc
     1801 tcggcagcgg catgcgcgcg gtcaagccga tcccgtctaa gaacggcagt tgacgagggc
     1861 ggagggggcg atagaatgta agcgttcgct tactcaaggt cgcggccgaa gcccgggagg
     1921 tgcgcatggc ggagggacat cgagtcgcag gccgcagcgg gcgggcggcg cacgtggcct
     1981 tgggactgct gtcgggcctc gccgtgctgg cggcggcggg cggcgatgcc cgggcggagc
     2041 cgacggcgca cgccgtcgcc ggctacctct atgcggtcgg cgacgccgag acggtgcatg
     2101 aggtgggcga gcttgcgctc aggcgctcat cgatggttgc cgccttcgcc gccgacgacc
     2161 cgtcgtcggc cctgcacctg gcgacgttca cctgcagctt cgtcagccac accaccaccg
     2221 acgcgagcgt ccgccatcag gaccgcgtcg gcgccgccgg cgcctgcctg gtgaccgacc
     2281 gcgacggtga cacccatgtc gccgagtggc agcgcccgcc cggcgccgag ctcggcacct
     2341 ggacggtggt gcgcggcacc ggcaaatggc gcgaggcgac cggcaccggc acctatgaca
     2401 tcctgttcct gagcccgccg cccaagcccc agctccgctt cac
//
LOCUS       DASWHU010000124         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3014587, whole genome shotgun sequence.
ACCESSION   DASWHU010000124 DASWHU010000000
VERSION     DASWHU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014587"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGB88_04395"
     CDS             complement(<1..587)
                     /locus_tag="VGB88_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646713.1"
                     /translation="MATAAAMAQALAASAAENVATELALRLDSESRRLEAAATLTVTG
                     GGPALLSLGESFRVSRLTVDGIDQSVPARRGARLEIALGEPGRHQVKLDYAGVLAPAT
                     GPDQGPLGPQEAVAGADGSFLPPGDWWPALDGAAGDFRLSIRVPAGQVATATGRRVSE
                     RHDGNGYAVTFEGGARQQPPAVFAGPYAIAERIAHG"
     gene            complement(625..1821)
                     /locus_tag="VGB88_04400"
     CDS             complement(625..1821)
                     /locus_tag="VGB88_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX9646714.1"
                     /translation="MCRAVAMVLAFLAASPGAHAGAGVAACAEAGTWIDPASGSTVTV
                     DRVVAAAAKRSAVLLGEVHDDADHHRWQLFMLAALHAQHPNIVIGLEMLPRRAQPVLD
                     AWVAGALTPEELVEQSEWTELWGFDAEIYLPILHFARQYRVPLVALNVERSLVAKVGE
                     IGWSAVPGAEREGVSDPTPAADGYVDMLARVYAAKQALASGAAAPVEAAEGTDLSAVR
                     ADPAFARFVEAQLTWDRAMAQAIAAAHGTAGAPLVVALVGIGHAEHRFGVAHQLADLG
                     VGETGVLLPVPQACEDLDPAVADAVFVTGDGEKAAAKPRLGVVIETAASGVSVREVAP
                     NSVAETAGIEAGDVIAMAAGVDVHTTADLIAIVRRQAPGTWLPIVVRRDGAAVELVAK
                     FPVAFE"
BASE COUNT          296 a          783 c          672 g          279 t
ORIGIN      
        1 ccgtgcgcga tgcgctcggc aatcgcatag ggacccgcga acacggccgg cggttgttgc
       61 cgggcaccgc cctcgaatgt caccgcatat ccgttgccat cgtgccgctc ggacacgcgg
      121 cggccggtcg cggtcgcaac ctggccggcc ggaacgcgga tcgacaggcg gaaatcgccg
      181 gccgccccgt cgagggccgg ccaccagtcg cctggcggca ggaagctgcc gtccgcgccg
      241 gcaacggcct cctgcgggcc aagaggcccc tggtcggggc ccgtcgccgg ggcgagcacg
      301 cccgcgtagt ccagcttgac ctggtgccgg ccgggctcgc cgagggcgat ctccagtctt
      361 gcgccgcggc gcgccggcac ggactggtcg atgccgtcga ccgtgagccg gctcacgcgg
      421 aacgactcgc cgagcgacag cagtgccggc ccgccgccgg tcaccgtcag cgtcgccgcc
      481 gcctcgagcc ggcgggactc cgaatcgagc ctcagggcga gctccgtcgc aacgttttcg
      541 gcggccgatg cagcaagagc ttgcgccatc gccgcggccg tcgccaccag cccgacaagg
      601 cagggcccga gtctcaccct cgtcctattc gaaagcgacc gggaacttgg ccacaagctc
      661 gaccgccgcg ccgtcgcggc gcaccactat cggcagccac gtcccgggcg cttggcgccg
      721 gacgatcgcg atcaggtcgg cggtcgtgtg tacgtccacg cccgcggcca tcgcgatcac
      781 gtcgccggcc tcgatgccgg cggtctcggc gacgctgttc ggggcgacct cgcgcactga
      841 cacccccgat gccgcggtct cgatcacgac gccgagccgc ggcttcgccg ccgccttctc
      901 cccgtcgccg gtcacgaaca ccgcatcggc gaccgctggg tccaggtctt cgcatgcctg
      961 cggcaccggc aaaagcaccc cggtctcgcc gaccccgagg tcggcgagct gatgggcgac
     1021 cccaaagcgg tgctcggcgt ggccaatgcc gaccagggcg acgaccagcg gcgctccggc
     1081 cgtgccgtgc gcggcggcga tcgcctgggc catcgcccgg tcccaggtca gctgggcctc
     1141 gacgaacctc gcgaatgcgg ggtcggccct gactgccgac aggtccgtgc cctcggccgc
     1201 ctccacgggc gcagccgccc ccgacgcgag cgcctgtttg gcggcataaa cacgtgccag
     1261 catgtcgaca tagccgtccg ccgcaggcgt cgggtcgctc acgccctcgc gctcggcgcc
     1321 cggcaccgcc gaccacccta tctcgccgac cttggcgacc aggctgcgct cgacgttgag
     1381 cgccaccagg ggcacccggt actgacgggc gaaatgcagg atcggcagat aaatttcagc
     1441 gtcgaagccc cacaactcgg tccattcgct ctgctcgacg agctcctcgg gggtgagcgc
     1501 gcccgccacc caggcgtcga gcacgggctg ggcgcgccgc ggcagcatct cgagcccgat
     1561 gactatgttc gggtgctgcg cgtgcagcgc cgccagcatg aacagttgcc agcggtggtg
     1621 gtcggcatcg tcgtggacct cgccgagcag cacggccgac cgcttcgccg ccgccgccac
     1681 cacgcggtcc acggtcaccg tcgagccgct cgcgggatcg atccaggtac cggcctcagc
     1741 acacgccgcg accccggcac cggcgtgcgc gcctggcgag gcggcaagga acgccagcac
     1801 catcgcgacg gcacgacaca tcgtcatcgc gactgccggg gccatcgccg cttttcgctt
     1861 cgtgtcacca tcgtcaccca ttgatcccaa acccgctccc gatcgccggc gcgcgcggtc
     1921 agttaaccat tccctcattt gtcggccgaa aaataaggat gcgatcgagc gatggcgatt
     1981 gcgagttcgc ctcgagcgcc cgatagttca tcccctcggt ggccgcgctc
//
LOCUS       DASWHU010000125         8490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1533367, whole genome shotgun sequence.
ACCESSION   DASWHU010000125 DASWHU010000000
VERSION     DASWHU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533367"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VGB88_04405"
     CDS             complement(<1..1104)
                     /locus_tag="VGB88_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9646715.1"
                     /translation="MTPAEVKARLAAADGEIALLDVRERGAHARGHPLFAASLPLSRI
                     ELDIDRLAPCRAASLVLLDDGGGDDRARRAAERLAGFGYADIALLDDGIAGWRAAGEE
                     LFDGVSVPSKAFGEYVELHCHTPHVTAPELARLKADGARIVILDSRPLEEYRRMSIPG
                     GIDVPGAELVHRVRDLAPDPDTLVVVNCAGRTRSIIGAQSLINAGLPNTVVALENGTM
                     GWALAGYELEHGREHAAPPPSADARRWARAAAARVAERFRVPRIDRAALARWQDEGAR
                     RTLYLCDVRTAEEYQAGHLPGARHAPGGQLVQASDSFIPVYGARVVLVDDDEVRAAMT
                     ASWLIQMGRREVHVLAGGIGADGLEPGPEPRTVL"
     gene            complement(1119..1739)
                     /locus_tag="VGB88_04410"
     CDS             complement(1119..1739)
                     /locus_tag="VGB88_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX9646716.1"
                     /translation="MAATADTDTRLTDAHRAAIAGCLGDVRAVIAEDGVTRKARAKVK
                     ARLLRLAARDDLFNYETFPVHSDDADKMSTIWLLAEDDDHAFALFGVAERTGKMSPPH
                     DHTTWAVIAGIEGEELNRFYDRLDDGAVPGRAEIREARQEVVAKGTGVALMPDDIHSI
                     HCVSEGPTFNFHLYGRSLRHLPERKMFNMRDGTTRPFPEQPNIRRA"
     gene            complement(1948..2982)
                     /locus_tag="VGB88_04415"
     CDS             complement(1948..2982)
                     /locus_tag="VGB88_04415"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX9646717.1"
                     /translation="MASTRGALIKLIGIAALALAVAAGGAPALAQQQPAGKDVQLLPP
                     PGGQDNDVEVWRAVRGGVTGSVSIPDKKAGVLVQSGGQAWRAIRNGPVSTYGVWLLLG
                     TVALLALFFALRGRIRIEHGRSGRRVLRFAGFERLVHWITAVSFILLALSGLNMLYGR
                     YVLKPYMGAEGFSALTMAGKVLHDYVGFVFIAGIAIMLVMWIRHNVPRVVDLAWLARG
                     GGMVGHDHPPAYKFNAGQKIIFAAVIIVGGMISYSGVSLVFPFETAATVQGMQDLQLW
                     HAVLSLVMMALIIAHIYIGTLGMEGAFEAMRSGYVDETWAREHHSLWVAELEAEGKAA
                     PEGARAAPAE"
     gene            complement(2986..3375)
                     /locus_tag="VGB88_04420"
     CDS             complement(2986..3375)
                     /locus_tag="VGB88_04420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9646718.1"
                     /translation="MNKRHNPTAVAPPIGTYSHGIEVAPGARWLHVAGEVGMAADGTV
                     SKGFEVQAEQAWRNVVAVLAAAGMGVEHLVHVNHWLVDQGHFEAYAKVRAKFLGDARP
                     ASTLMIVKALAKPEFLVEVGGVAAKVD"
     gene            complement(3401..3592)
                     /locus_tag="VGB88_04425"
     CDS             complement(3401..3592)
                     /locus_tag="VGB88_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646719.1"
                     /translation="MTRTAGSRTAAIVLALLLLLACRDEEQGRKLGYQAGVYQGAEMP
                     ELDPETRDALRQRAQNQDF"
     gene            complement(3612..4190)
                     /gene="fdh3B"
                     /locus_tag="VGB88_04430"
     CDS             complement(3612..4190)
                     /gene="fdh3B"
                     /locus_tag="VGB88_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011047528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEX9646720.1"
                     /translation="MARMKFLCDAERCIECNACVTACKNEHEVPWGINRRRVVTINDG
                     QPGERSISVACMHCSDAPCKAVCPVDCFYTTEDGVVLHSKDLCIGCGYCFYACPFGAP
                     QYPQAGNFGSRGKMDKCTFCAGGPEDSAYEYGRDRLSEGKLPLCAEMCSTKALLAGDG
                     DTLSNIYRERVIARGFGSGAWGWGTAYAGDDA"
     gene            complement(4219..7089)
                     /locus_tag="VGB88_04435"
     CDS             complement(4219..7089)
                     /locus_tag="VGB88_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX9646721.1"
                     /translation="MLIKRRSEAAEGGSRSTVRTGHGAATVDRRAFLKASGIAAGGAA
                     ALAGGLPLTRVTKSQAAAEAGGEATIVKTVCTHCSVGCTVTAEVENGVWTGQEPSFDS
                     PFNLGAHCAKGASVREHAHGERRLKYPMKKDGGAWTRIGWDQAIEEIGDRLLKIRDHA
                     GPDSVYWLGSAKHSNEQAYLFRKFAAFWGSNNLDHQARICHSTTVAGVANTWGYGAMT
                     NSYNDIHRSRAILFIGGNPAEAHPVSLLHILKGKEQNGAKVIVCDPRFTRTAAHADEY
                     VRLRPGTDVPLVWGLLWHIFGNGWEDQQFIRQRVWGMDQVRAEVAKWTPQEVERVTGV
                     PGSQVKRVAALLANNRPGTVVWCMGGTQHTVGNNNTRAYCILQLALGNMGVSGGGTNI
                     FRGHDNVQGATDFGVVCDSLPGYYGLAEGAWKHWARVWDVDYESLKSRFASQELMETA
                     GMPVSRWFDGVLEGANVAQPAPLQAMIFWGHAPNSQTRLPDMKQAMEMLDTLVVIDPY
                     PTMTAVMQDRTDGVYLLPAATQFETYGSVTASNRSLQWREKVVDPLFESRTDQEIMYR
                     FAAKFGFAEEMFKHIQVDGNEPLIEDITREFNRGMWTIGYTGQSPERLRLHMANQHTF
                     DKTTLQAVGGPCDGDYYGLPWPAWGPPEMKHPGTPILYDTSKTVAEGGLTFRARFGVE
                     RDGENLLAEGSYSAGSEIEDGYPEFTMEMLRKLGWDADLTADERAAVERVGGDAANWK
                     TDLSGGIQRVAIKHGCAPFGNAKARCVVWTFPDPVPVHREPLYSNRRDLVADYPTYED
                     KVFFRLPFLYKSIQDKDFATDYPLILTSGRLVEYEGGGDESRSNPWLAELQQHMFAEV
                     NPFDANNAGFKDGDDVWVEGPEGGRVKVMAMVTERVGRGVVFMPFHFGGHWMGEDQRA
                     KYPPGSDPYVLGEASNTAQTYGYDSVTAMQETKATLCRIYPA"
     gene            complement(7112..7327)
                     /locus_tag="VGB88_04440"
     CDS             complement(7112..7327)
                     /locus_tag="VGB88_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009605125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9646722.1"
                     /translation="MRSDGKRTRGQDGLHRRDFLKTATGGTAAGVVTAVLAGGTAPAE
                     AAAEPAAGRSRYRETEHVRRAYRLARF"
     gene            complement(7369..7998)
                     /locus_tag="VGB88_04445"
     CDS             complement(7369..7998)
                     /locus_tag="VGB88_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009817712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX9646723.1"
                     /translation="MQANQLVASQKTGAQLRPAAEELARAGWYRLLATLLGSPPGRDV
                     LDLIGTLSAGPTPLGAAAEALAAAARKAEPKALEREFTDLFIGIGEGELVPYGSYYLT
                     GFLHERPLARLRADLSALGLARAEGVYEPEDGIAGLCEVMAALIDGSLDAPVPLDRQR
                     RFFDSHLAPWADRFFADLESAQSAAFYRHVARLGRVIIKLESEAFEISG"
     gene            complement(8137..>8490)
                     /locus_tag="VGB88_04450"
     CDS             complement(8137..>8490)
                     /locus_tag="VGB88_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEX9646724.1"
                     /translation="PLAKLPPIDELGAESDYTGFLAREVPVELRRQALRKLWRSSPTL
                     ANLDGLNDYEDDYRSLGAGKLVRTVFEVGKGLARRLAGDGAARAPAPAANAGSGDEPP
                     ATQEAAQAPTSDKPA"
BASE COUNT         1197 a         2998 c         2987 g         1308 t
ORIGIN      
        1 gagcacggtg cgcggctcgg ggccgggctc gagcccgtcc gcgccgatcc cgccggcgag
       61 cacgtggacc tcgcgccggc ccatctggat cagccacgac gcggtcatgg cggcgcgcac
      121 ctcgtcgtcg tcgaccagga ccacgcgcgc gccgtagacc gggatgaagc tgtcgcttgc
      181 ctggacgagc tgtccgcccg gggcgtggcg cgcgccgggc aggtggccgg cctggtactc
      241 ctcggcggtg cgcacgtcgc acagatagag cgtgcggcgc gcgccctcgt cctgccagcg
      301 cgccaacgcg gcgcggtcga tgcgcggcac gcggaaccgc tccgcgaccc gtgccgcggc
      361 cgcgcgcgcc cagcgccgcg cgtccgcgct cggcggcggc gcggcgtgct cccggccgtg
      421 ctcgagctca tagccggcca aggcccagcc catggtgccg ttctcgagcg ccaccaccgt
      481 gttgggcagg ccggcgttga tcagcgactg ggcgccgatg atgctgcgcg tgcgcccggc
      541 gcagttgacc accaccaggg tgtcggggtc cggcgccaag tcgcggacgc ggtggaccag
      601 ctcggccccg gggacgtcga tgccgcccgg gatgctcatg cgccggtact cctcgagcgg
      661 gcggctgtcg aggatcacta tccgggcgcc gtcggccttg agccgggcga gctcgggcgc
      721 ggtgacgtgc ggggtgtggc aatgcagctc gacatactcg ccgaacgcct tgctcggcac
      781 ggaaacgccg tcgaacagct cctcgcccgc ggcgcgccag ccggcgatgc cgtcgtcgag
      841 aagggcgatg tccgcatagc cgaagccggc gaggcgctcg gctgcgcggc gcgcgcggtc
      901 gtcgccgccg ccgtcgtcga gcagcaccag cgacgcggcg cggcacggcg ccagccggtc
      961 gatgtcgagc tcgatccggc tcagcggcag gctggcggca aacagcgggt ggccgcgggc
     1021 gtgcgcgccg cgctcgcgta cgtcgagcag cgcgatctcg ccgtccgccg cggcgaggcg
     1081 cgccttgacc tcggccgggg tcatcgaccg cccgaggatt aggcgcggcg gatgttgggc
     1141 tgctcgggga acggccgcgt ggtgccgtcc ctcatgttga acatcttgcg ctcgggcagg
     1201 tggcggaggc tgcgcccgta gagatggaag ttgaaggtcg gcccttccga gacgcagtgg
     1261 atgctgtgga tgtcgtcggg catcagcgcc accccggtgc ccttcgcgac cacctcctgg
     1321 cgcgcctcgc ggatctcggc gcggccgggc acggcgccgt cgtcgagacg gtcgtagaac
     1381 cggttcagct cctcgccctc gatgccggcg atcaccgccc aggtggtgtg atcgtgcggc
     1441 ggcgacatct tgccggtgcg ctcggcgacc ccgaacaggg cgaaggcatg gtcgtcgtcc
     1501 tcggccagca gccagatcgt gctcatcttg tcggcgtcgt ccgaatgcac gggaaatgtt
     1561 tcgtagttga acaggtcgtc gcgcgcggcg agcctgagca gccgcgcctt gactttggcc
     1621 cgggccttcc gggtcacgcc gtcctcggcg atgaccgcgc gcacgtcgcc gagacagccg
     1681 gcgatcgccg cgcggtgggc gtcggtcagg cgggtgtcgg tatcggcggt cgcggccatc
     1741 ttccaatcct cccggatcca agcgtgagat ccgtttgggc ttatagcgag accggggcgc
     1801 aactagcaac gcccgaagat taagcaccga tcgtcacccc ggccgaagag ccggggtcca
     1861 ttcgatggtg caactggttc catggatgcc ggctcggcgc gccggccaag gccggcgctt
     1921 ggccggcatg acgatcgagg ttccgtatta ctccgccggc gcggccctgg cgccctcggg
     1981 ggcggccttg ccttcggcct cgagctcggc gacccacagg ctgtggtgct cgcgcgccca
     2041 ggtctcgtcg acatagcccg accgcatcgc ctcgaacgca ccctccatgc cgagcgtgcc
     2101 gatgtatatg tgggcgatga tcagcgccat catcaccagc gacaggaccg cgtgccagag
     2161 ctgcaaatcc tgcatgccct ggacggtggc cgcggtctcg aacgggaaga ccaggctgac
     2221 gccggaatag ctgatcatgc cgccgacgat gatcaccgcg gcgaagatga tcttctgccc
     2281 ggcattgaac ttgtaggccg gcggatggtc gtggccgacc atgccgccgc cgcgcgccag
     2341 ccaggcgagg tcgaccacgc gcgggacgtt gtggcggatc cacatcacca gcatgatcgc
     2401 gatgccggcg atgaacacga agccgacgta gtcgtgcagc accttgccgg ccatggtcag
     2461 cgccgagaac ccctccgcgc ccatgtaggg cttgagcacg taacgcccgt acagcatgtt
     2521 cagccccgac agcgcgagca ggatgaagct gaccgcggtg atccaatgta ccagtcgctc
     2581 gaagccggcg aagcgcagca cccgccgccc ggagcggccg tgctcgatgc ggatgcggcc
     2641 gcgcaacgcg aagaacagcg ccagcaaggc gaccgtgccg agcagcagcc ataccccgta
     2701 ggtcgacacc gggccgttgc ggatcgcccg ccacgcctgg ccacccgact ggaccagcac
     2761 gcccgccttc ttgtcgggga tcgagacaga gccggtgacg ccgccgcgca ccgcccgcca
     2821 gacctcgacg tcgttgtcct gcccgcccgg cggcggcagc agctggacgt ccttgcccgc
     2881 cggctgctgc tgggcgagcg cgggcgcacc gccggcggcg accgccagcg ccagggccgc
     2941 gatgccgatc agcttgatca gcgcgccgcg cgtcgatgcc atcggtcagt ccaccttcgc
     3001 cgccacgccg ccgacctcga ccaggaactc cggcttggcc agcgccttga cgatcatcag
     3061 cgtcgacgcc ggccgggcgt cgccaagaaa cttcgcccgc accttggcat aggcctcgaa
     3121 atggccctgg tcgaccagcc agtggttgac gtggaccaga tgctcgaccc ccatgcccgc
     3181 cgcggccagc accgcgacca cgttgcgcca cgcctgctcg gcctggacct cgaagccctt
     3241 ggacacggtg ccgtcggcgg ccatgccgac ctcgccggcg acgtgcagcc agcgggcgcc
     3301 gggcgccacc tcgatgccgt ggctgtaggt gccgatcggc ggcgcgacgg cggtcgggtt
     3361 gtggcgcttg ttcacggttg cctccggcac gaatggtcga tcagaaatcc tggttctgcg
     3421 cgcgctggcg caaggcgtca cgggtctcgg ggtcgagctc gggcatctcg gcgccctgat
     3481 agactcccgc ctggtagccg agcttgcggc cctgttcctc atcgcggcag gccagcaaca
     3541 agagcagtgc gaggacgatc gcggccgtcc ggctgccggc ggtgcgcgtc atcgtcaccc
     3601 gaccgccggc ctcaggcgtc gtcgccggcg taggccgtgc cccagcccca ggcgcccgag
     3661 ccgaagccgc gcgcgatcac ccgctcgcgg tagatgttgg atagcgtgtc gccgtcgccg
     3721 gcgagcagcg ccttggtcga gcacatctcg gcgcacagcg gcagcttgcc ttccgacagc
     3781 cggtcgcggc cgtactcgta ggccgagtcc tcgggcccgc cggcgcagaa ggtgcacttg
     3841 tccatcttgc cgcggctgcc gaagttgccg gcctgcggat attgcggcgc cccgaacggg
     3901 caggcgtaga agcagtagcc gcagccgatg cacaggtcct tggagtgcag caccacgccg
     3961 tcctcggtgg tgtagaagca gtcaacgggg cagaccgcct tgcacggcgc gtccgagcag
     4021 tgcatgcagg cgaccgagat cgagcgctcg ccgggctggc cgtcgttgat ggtgaccacg
     4081 cgccggcggt tgatgcccca cggcacctcg tgctcgttct tgcacgcggt gacgcaggcg
     4141 ttgcattcga tacagcgttc ggcgtcgcac aggaacttca ttctggccat ggttcgggct
     4201 ccttcctccg cgcgcgcgtt acgccgggta gatccggcac agcgtggcct tggtctcctg
     4261 catcgccgtg acgctgtcgt aaccgtaggt ctgggcggtg ttggacgcct cgccgagcac
     4321 gtaggggtcg ctgcccggcg ggtacttggc gcgctggtcc tcgcccatcc aatggccgcc
     4381 gaagtggaac ggcatgaaca ccacgccgcg gccgacccgc tcggtgacca tggccatgac
     4441 cttgacccgg ccgccctccg gcccctcgac ccagacgtcg tcgccgtcct tgaagccggc
     4501 gttgttggcg tcgaacgggt tgacctcggc gaacatgtgc tgctgaagct cggcgagcca
     4561 cgggttcgac cgcgactcgt cgccgccgcc ctcgtactcg accagccggc ccgaggtcag
     4621 gatcagcggg tagtcggtcg cgaagtcctt gtcctgaatc gacttgtaga ggaacggcag
     4681 gcgaaagaac accttgtcct cgtaggtcgg gtagtcggcg accaggtcgc gccggttgct
     4741 gtacagcggc tcgcggtgga ccggcaccgg atcggggaag gtccacacca cgcagcgcgc
     4801 cttggcgttg ccgaacgggg cgcagccgtg cttgatcgcg acccgctgga tgccgccgga
     4861 caggtcggtc ttccagttgg cggcatcgcc gccgacccgc tcgacggcgg cgcgctcgtc
     4921 ggcggtcagg tccgcgtccc agccgagctt cctgagcatc tccatggtga actcgggata
     4981 gccgtcctcg atctccgagc ccgccgagta cgagccctcg gcgagcaggt tctcgccgtc
     5041 gcgctcgacc ccgaagcggg cgcggaaggt gagcccgccc tcggccaccg tcttagacgt
     5101 gtcgtacagg atcggcgtgc ccggatgctt catctcgggc gggccccagg ccggccacgg
     5161 caggccgtaa tagtcgccgt cgcacggccc gcccaccgcc tgcaacgtgg tcttgtcgaa
     5221 ggtgtgctgg ttggccatgt gcaggcgcag ccgctcgggc gactggccgg tgtagccgat
     5281 cgtccacatg ccgcggttga actcgcgcgt gatgtcctcg atcagcggct cgttgccgtc
     5341 gacctggatg tgcttgaaca tctcctcggc gaagccgaac ttggcggcga agcggtacat
     5401 gatctcctgg tcggtgcgcg actcgaacaa gggatccacc accttctcgc gccattgcag
     5461 cgagcggttg gacgcggtca ccgagccgta ggtctcgaac tgggtcgccg ccggcaacag
     5521 gtagacgccg tcggtgcggt cctgcatgac cgcggtcatg gtcgggtagg gatcgatcac
     5581 gaccaaggtg tcgagcatct ccatcgcctg cttcatgtcc ggcaggcggg tctgcgagtt
     5641 gggcgcgtgg ccccagaaga tcatcgcctg gagcggcgcc ggctgcgcga cgttggcgcc
     5701 ctcgagcacg ccgtcgaacc agcgcgacac cggcatgccg gcggtctcca tcagctcctg
     5761 gctggcgaag cggctcttga gggactcgta gtcgacatcc cacacccgcg cccagtgctt
     5821 ccaggcgcct tccgccaggc cgtaatagcc gggcagcgag tcgcacacca cgccgaaatc
     5881 ggtcgcgccc tggacgttgt cgtggccgcg gaagatgttg gtgccgccgc ccgagacgcc
     5941 catgttgccg agcgcgagct ggaggatgca gtaggcgcgg gtgttgttgt tgccgacggt
     6001 gtgctgggtg ccgcccatgc accagaccac cgtgcccggg cggttgttgg cgagcagcgc
     6061 cgccacccgc ttgacctgcg agcccggcac gccggtcacg cgttcgacct cctggggcgt
     6121 ccacttggcg acctcggcgc gcacctggtc catgccccag acgcgctggc ggatgaactg
     6181 ctggtcctcc cagccgttgc cgaagatgtg ccacaacagg ccccacacca gcggcacgtc
     6241 ggtgccgggg cgcaggcgga catactcgtc ggcgtgcgcc gcggtgcgcg tgaagcgcgg
     6301 gtcgcagacg atcaccttgg cgccgttctg ctccttgccc ttgaggatgt gcagcagcga
     6361 caccggatgc gcctcggccg ggttgccgcc gatgaacaag atcgcgcgcg agcggtggat
     6421 gtcgttgtag ctgttggtca tggcgccgta gccccaggtg ttggcgacgc cggcgaccgt
     6481 ggtcgagtgg cagatgcgcg cctggtggtc gaggttgttc gagccccaga acgccgcgaa
     6541 cttgcggaac aggtaagcct gctcgttcga gtgcttggcc gagcccagcc agtacaccga
     6601 gtccgggccg gcgtggtcgc ggatcttgag cagccggtcg ccgatctcct cgatcgcctg
     6661 gtcccagccg atccgtgtcc acgcgccgcc gtccttcttc atcgggtatt tgagccgccg
     6721 ttcgccgtgg gcgtgctcgc gcacgctggc gcccttggcg cagtgggcgc cgaggttgaa
     6781 cgggctgtcg aaggacggct cctggccggt ccagactccg ttctcgacct cggcggtgac
     6841 cgtgcaaccg accgagcagt gggtacacac cgtcttgacg atggtggcct cgccgcccgc
     6901 ctcggccgcg gcctgggact tcgtgacccg ggtcagcggc aggccgccgg caagcgccgc
     6961 ggcgccgccg gcggcgatgc ccgaggcctt gaggaacgcg cgccggtcga cggtcgcggc
     7021 gccgtggccc gtgcgcacgg tgctgcgcga gccgccctcg gcggcctcgg aacgtcgctt
     7081 gatgagcatg gctcgctccc ctgaggctcg atcagaaccg cgccagccgg taggcccggc
     7141 gcacgtgctc ggtctcgcgg taacggctgc gcccggccgc gggctcggcg gccgcctcgg
     7201 ccggcgcggt gccgccggca agcaccgcgg tcaccacccc cgccgcagtg ccgccggtcg
     7261 cggtcttgag gaaatcgcgc cggtgaaggc cgtcctgccc gcgcgtccgc ttgccatcgc
     7321 tgcgcatcgt caatccccgt tccaatgcgg cgagaatact aaaaaatctc agccgcttat
     7381 ttcaaacgcc tcagattcca gcttgatgat cacgcggccc agccgggcga cgtggcgata
     7441 gaacgcggcc gactgcgcgc tctccaggtc ggcgaagaag cggtcggccc agggcgcaag
     7501 gtgggaatcg aagaaccggc gctggcggtc gagcggcacc ggcgcatcaa ggctgccgtc
     7561 gatcagtgcc gccatcacct cgcacaggcc ggcgataccg tcctcgggct cgtacacgcc
     7621 ctccgcccgc gccaagccga gcgcgctgag gtcggcgcgc agccgcgcga gcgggcgctc
     7681 gtgcaggaac ccggtcaggt agtacgaccc gtaggggacc agctcgcctt cgccgatgcc
     7741 gatgaacagg tcggtgaact cgcgctcgag cgccttcggc tcggccttgc gcgccgccgc
     7801 ggcgagcgct tcggccgccg cgccgagcgg cgtcgggccg gcggaaagcg tgccgatcag
     7861 gtcgagcaca tcccggccgg gcgggctgcc gagcaaggtc gcgagcagcc gataccagcc
     7921 ggcccgggca agctcctcag cggccggcct aagctgtgcg ccagtcttct ggcttgcgac
     7981 cagttggttt gcctgcaacg gcgccgcctc ccacccacgt cttatatgtg ccgcctgcga
     8041 tgtggccgcg gcgacacgat tatgggccac gtctcgggtg gcgacgacga ttgtggccca
     8101 gtgccgcggg ccggtcaact ggaccccaat cggcgcctac gccggcttgt ccgaggtcgg
     8161 cgcctgcgcg gcctcctgcg tcgccggagg ctcgtcgccg gacccggcat ttgcggccgg
     8221 ggctggcgcg cgggccgcgc cgtcgccggc gagcctgcgg gccagcccct tgccgacctc
     8281 gaataccgtg cggaccagct ttcccgcgcc gaggctacgg tagtcgtcct cgtagtcgtt
     8341 aaggccgtcg aggttggcga gggtcggcga gctccgccac agcttgcgca gcgcctggcg
     8401 cctgagctcg accggcacct cgcgggcgag gaacccggtg tagtcggact cggcgccgag
     8461 ctcgtcgatc ggcggcagct tggcgagcgg
//
LOCUS       DASWHU010000126         8723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3375979, whole genome shotgun sequence.
ACCESSION   DASWHU010000126 DASWHU010000000
VERSION     DASWHU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3375979"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1863
                     /gene="aepX"
                     /locus_tag="VGB88_04455"
     CDS             178..1863
                     /gene="aepX"
                     /locus_tag="VGB88_04455"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HEX9646725.1"
                     /translation="MTLPLVSPRADAGPLTKTRAFRALLASPELEFLCEAHNGLSARI
                     AEEAGFKGLWASGLAISAALGVRDSNEASWTQVLEVVEFMADATSVPIMLDGDTGYGN
                     FNNMRRLVRKLERRGVAAVCIEDKLFPKTNSFLAGEAQALADIGEFCGKIRAGKEARE
                     DPDFAIVARCEAFIAGWGLDEALRRAEAYHAAGADAILIHSAKRTAEEVLAFLDAWDG
                     RAPVVLVPTRYYTTPTEAFRAHGASLVIWANHLVRAAITAMQHAAEAIHADQNLLAVE
                     DAIAPVAEVFRLQGADELDRAEKVYLPQAPAARAVVLAAARGSELGALTEALPKAMLP
                     VADQPLLAHIAAAYRRAGIKDITVVRGFAKDRVALTGLAFVDNDAFADTQELASLACA
                     LDARADAGALVVSYGDVLFRRHLLDMLDDALAVPGGGEALAAIVVDTNWRQSRNRGAD
                     RHPDYVRCSRAFGRAAYSGPVWLEEMGERPGARTDGEWMGLVRFTPAGTAALRAAVAD
                     WRARDAAGFAAAGMSELLTRMAAAGGRILVVYTTGHWLDIDHVDDLVLAGSFV"
     gene            1860..3020
                     /gene="aepY"
                     /locus_tag="VGB88_04460"
     CDS             1860..3020
                     /gene="aepY"
                     /locus_tag="VGB88_04460"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX9646726.1"
                     /translation="MITAPGFVGAARARGFGVYSGVPCSYLTPLINHVIGARELSYVA
                     AANEGDAVAVAAGASLGGRRGVAMFQNSGLGNAVSPLTSLTWTFRVPILVIVTWRGEP
                     GGAADEPQHELMGRITPAQLELMEIPWRRFPTEDGDIASALDAAEAHMARAGRPFALV
                     LSKGSVAPFPLAAPAPPPRAPAPALPPPAAGPPRPTRNDVLAALQRASAPGDVLIATT
                     GYTGRELYALDDRANQLYMVGSMGCASSLALGVALARPDRRVIAIDGDGAVLMRLGAL
                     STIGAVAAPNLVHVVIDNAMHESTGGQATVTATTDLCAVAAACGYARLARPAASEELA
                     ALLAEPAPGPLFVHMRVRPGVPDGLPRPRIAPAEVAARLARHIAAVPVRPAT"
     gene            3017..5635
                     /locus_tag="VGB88_04465"
     CDS             3017..5635
                     /locus_tag="VGB88_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9646727.1"
                     /translation="MSADLGGLGRRLDRAAGAVIEACLRAPLLVVAVALVLAAAALAA
                     AYHGLPINTSTSDMISDETPVMRTMDALKARFPGRSDNIVVLIEADTPDQARDAAEAL
                     VERLTARADLFDQVFSPGIGPFFDRNGLLYLDRDELLEVLDGIADSSPLIQRLRDDTT
                     LRGLFAVVGLASEDALGDAEAADNLDWLYGRIARVAEARLDGAPALLSWQEIMFGREA
                     EPEDLRTTVIVRPARIDYGTLLPGADAIGELRGIVREAGLTPAAGVRVSLTGGPALEL
                     DELATVQRGIALTGVLSLVLVVAILWAGLRSARMVAATLATLVIGLIWTAGFAALAVG
                     HLNLISVAFAVLFVSLGVDFMIHLALRVQEAAAGAVPDRISVAAGTRAVASSLGLCAL
                     TTAVGFYAFLPTAYRGVAELGVIAGTGMIVALLASLTVLPALLVLWPAKVPARRAVAP
                     PAVRLPRVSAPAVRGGALAGLVLALAALPWLSFDANPLNLQDPEAESVRAVRHLMAEG
                     ERDLWAGASLAADAGEAERLAAAFDALGEVDRAVWLGDLVPPDQDQRLDVIQDIAFAA
                     EPDPFATPYPAPSADEQRAAVAALAGALGKLGASLPLGDGGRALGRALARLEAAPDAA
                     IAGVDAGLVGALPARLQALDDALGAEPFGVDDLPEALRSRYRGTDGSLRVDVYPAEDV
                     IADNGALARFVAALRSVDPAVTDDPVIVFEAGRVVVASFVQALASALVLILLLLYVLF
                     RRLVDVLVVLAPLVLAGAITGAATVVLGMSFNFANVIVLPLLLGIGVDSGIHMVHRYR
                     MEPDRPVWRTSTARAVVFSALTTIAGFGSLALSPHPGAASMGALLLIGVVITVGCTLG
                     VLPALLPFTRPAGRAG"
     gene            5632..6636
                     /locus_tag="VGB88_04470"
     CDS             5632..6636
                     /locus_tag="VGB88_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9646728.1"
                     /translation="MKRLIAVLGLAGLALATGLIVWQGAGAVGSAVLAAGWGLAIVIA
                     VEILPLGIDALAWRTLIPAGVRPGVGVALWARWIRQSVGQLLPVAQVGGDVVGARMLY
                     LRGVPGEVAGATVVVDLTLGAATQLVYALTGLALLWARPGGDGVAAGVLIGGGVLVLG
                     LAGFVIAQRNGLFRFLARRLHAVSGGLLALVGSAERLDHAVRAVYARPWDLARNAALQ
                     LLSWTTGTLEVWLALRFIGHPVGLVEAYILQSLTRMVRSAAFPVPGGLGVQEAGLMVL
                     AGLLGLNPEIGLALSLIKRVRELVLGVPGLIAWQAAEGNHLLRRRRRLAAEAPEQGAS
                     "
     gene            6641..7408
                     /locus_tag="VGB88_04475"
     CDS             6641..7408
                     /locus_tag="VGB88_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX9646729.1"
                     /translation="MRAILFAAGVGRRLAAVADGLPKALLRLGGASLLERHLRILAHC
                     GIGEVVVVTGFGAEAVAGEVARLDTGASVRLVHNPDYRAGSILSQWAARATLAAGEAV
                     LLMDADVLYDQRLMARLIGSGHDDCFLLDRDIVPGEEPVKLCVRAGQLVDFRKRPSEP
                     HDWHGESVGFFKLGPGTARELVEATQRYVEAGRTEEYYDEALRDLLVAGPPGRFGFED
                     VTGLPWIEIDVPDDLDRAEREVLPRLEPLPPRPEAGR"
     gene            7405..8031
                     /locus_tag="VGB88_04480"
     CDS             7405..8031
                     /locus_tag="VGB88_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9646730.1"
                     /translation="MSHDTWAHRMIAPAVRPLARTPVTPNQLTAVRLAFGIAAAGAFA
                     AGGPVWPQAGSALWLASMLFDRADGILARLTGKTSAWGHKFDLFADFTSTTLVFAGIG
                     IGLRGGTLGPWAPALGAVAGLSVAAIFALVYRIQGLLPAGRSAVPSAAGFDADDVLFV
                     VAPVAWLGWLQPFLVTAAVGAPLAAALALATLAQLRRAQRSNTVVGNE"
     gene            complement(7998..8543)
                     /locus_tag="VGB88_04485"
     CDS             complement(7998..8543)
                     /locus_tag="VGB88_04485"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX9646731.1"
                     /translation="MTPTAPTARGLRHRLALGMMVIAAPIVCAGGAAAEERYGEPDFI
                     TVGAGAFDFDDDMAAAELDLQVRLNTRLWVFRPQLGLFVTDDAGFYAYAGIFTDIYFG
                     RRLVLSPSIGAGGYGEGNGKDLGGAVEFRSAVELAYRFDDSSRLGLQFGHLSNAGLYD
                     DNPGEEFLILNYSLPTTVFDR"
     gene            complement(8554..>8723)
                     /locus_tag="VGB88_04490"
     CDS             complement(8554..>8723)
                     /locus_tag="VGB88_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX9646732.1"
                     /translation="PIETACDLVRAWPEAELHVVQDAGHSSMEPGIRAKLVEIMDRLR
                     DHGTGAPGPRG"
BASE COUNT          966 a         3225 c         3257 g         1275 t
ORIGIN      
        1 tcgaacccac gaccccagga ttaggaatcc tgtgctctat ccggctgagc tacgggcgcg
       61 ccgggcgccc ttcttagcat ggcgcggatg cgcctgcgag cctggccggt gggatcgccg
      121 ccggcgcgcg cccgggcgtc cttgttcgag gccggcttcc tcgtatatga agcttccatg
      181 accttgccgc tcgtgagtcc gcgcgccgac gccgggccgc tgaccaagac ccgggccttc
      241 cgcgcgctcc tcgcctcgcc cgagctcgag ttcctgtgcg aggcgcataa cggcttgagc
      301 gcgcgcatcg ccgaggaggc gggcttcaag ggcctgtggg cgtcggggct cgcgatctcg
      361 gccgcgctcg gcgtgcgcga cagcaacgag gcgagctgga cccaggtgct cgaggtggtc
      421 gagttcatgg ccgacgccac ctccgtgccg atcatgctcg acggcgacac cggctacggc
      481 aacttcaaca acatgcggcg cttggtgcgc aagctcgagc ggcgcggcgt cgccgcggtg
      541 tgcatcgagg acaagctgtt ccccaagacc aacagcttcc tggccggcga ggcccaggcg
      601 ctggccgaca tcggcgagtt ctgcggcaag atccgcgccg gcaaggaggc gcgcgaggac
      661 cccgacttcg cgatcgtcgc gcgctgcgag gcgttcatcg ccggctgggg gctggacgag
      721 gcgctgcgcc gcgccgaggc ctaccatgcc gccggcgccg acgcgatcct gatccacagc
      781 gccaagcgca ccgccgagga ggtgctcgcg ttcctggacg cctgggacgg gcgcgcgccg
      841 gtggtgctgg tgccgaccag gtactacacc acgccgaccg aggcgttccg cgcccacggc
      901 gcctcgctgg tgatctgggc caaccacctg gtgcgcgccg cgatcaccgc catgcagcac
      961 gcggccgagg cgatccacgc cgaccagaac ctgctcgcgg tcgaggacgc catcgccccg
     1021 gtcgccgagg tgttccgcct gcagggcgcc gacgagctcg accgggccga gaaggtctac
     1081 ctgccgcagg cgccggcggc gcgcgcggtg gtgctggcgg cggcgcgcgg cagcgagctg
     1141 ggcgcgctca ccgaagcgct gcccaaggcg atgctgccgg tcgccgacca gccgctgctc
     1201 gcccacatcg ccgccgccta tcggcgcgcc ggcatcaagg acatcaccgt ggtgcgcggc
     1261 ttcgccaagg accgggtggc gctgacgggc ctcgccttcg tcgacaacga cgccttcgcg
     1321 gacacccagg agctcgcctc gctcgcctgc gcgctggacg cgcgcgccga tgccggcgcg
     1381 ctggtggtct cgtacggcga cgtgctgttc cgccgccatt tgctggacat gctcgacgac
     1441 gcgctcgcgg tgccgggcgg cggcgaagcg ctggccgcga tcgtggtcga caccaactgg
     1501 cgccagagcc gcaaccgcgg cgccgaccgc cacccggact acgtccgctg cagccgcgcc
     1561 ttcgggcgcg cggcctattc gggcccggtg tggctcgagg agatgggcga gcgcccgggc
     1621 gcgcgcaccg acggcgagtg gatgggcctg gtccggttca cgcccgcggg cacggccgcg
     1681 ctcagggccg cggtcgccga ctggcgcgcg cgtgatgctg ccggcttcgc cgccgccggc
     1741 atgagcgagc tgctgacccg catggccgcg gccggcgggc gcatcctggt ggtctacacc
     1801 accggccact ggctcgacat cgaccacgtc gacgacctgg tgctcgccgg gagcttcgtt
     1861 tgatcaccgc gccgggcttc gtcggcgccg cgcgtgcgcg cggcttcggc gtctacagcg
     1921 gcgttccgtg ctcctacctg accccgttga tcaaccacgt catcggcgcg cgcgagctca
     1981 gctacgtggc ggcggcgaac gagggcgacg cggtcgcggt cgccgccggc gccagcctcg
     2041 gcggccggcg cggcgtcgcc atgttccaga actcgggcct cggcaacgcg gtcagcccgc
     2101 tcacctcgct cacctggacc ttccgcgtgc cgatcctggt catcgtcacc tggcgcggcg
     2161 agcccggcgg cgccgccgac gagccgcagc acgagctgat gggccggatc acgccggccc
     2221 agctcgagct gatggagatt ccctggcgcc ggttccccac cgaggacggc gacatcgcgt
     2281 ccgcgctcga tgccgccgag gcgcacatgg cgcgcgccgg gcggccgttc gcgctggtct
     2341 tatccaaggg atcggtggcg ccgttcccgc tcgccgcccc agcgccgccc ccgcgcgccc
     2401 cggcgccggc gctgccgccg ccggccgcgg gcccgccccg gcccacccgc aacgacgtgc
     2461 tggcggcgct gcagcgcgcc tcggcgcccg gcgacgtgct gattgccacc accggttaca
     2521 ccgggcgcga gctctacgcc ctcgacgacc gggccaacca gctctacatg gtgggctcga
     2581 tgggctgcgc ctcgagcctg gcgctcggcg tggcgctggc gcggccggat cggcgcgtga
     2641 tcgcgatcga cggcgacggc gcggtgctga tgcgcctcgg ggcgctgtcg accatcggcg
     2701 cggtggcggc gcccaacctg gtccacgtgg tgatcgacaa tgccatgcat gaatcgaccg
     2761 gcggccaggc gacggtgacc gcgaccaccg atctgtgcgc ggtcgccgcc gcctgcggct
     2821 atgcccgcct cgcccgcccg gccgcgagcg aggagctggc ggcactgctc gccgagccgg
     2881 cgccggggcc gctgttcgtg cacatgcgcg tccgccccgg ggtgcccgac ggcctgccgc
     2941 ggccgcgcat cgccccggcc gaggtggcgg cgaggctcgc ccggcacatc gccgccgtgc
     3001 cggtgcggcc ggcgacgtga gcgcggacct gggcggcctc ggccgccgtc tcgaccgcgc
     3061 cgccggcgcg gtgatcgagg cctgcctgcg cgcaccgctg ttggtcgtcg ccgtcgcgct
     3121 cgtgctcgcc gccgccgcgc tcgccgccgc ctatcatggc ctgccgatca acacctcgac
     3181 ctcggacatg atctccgacg agacgccggt catgcgcacc atggacgcgc tgaaggcgcg
     3241 cttcccgggg cgcagcgaca acatcgtggt actgatcgag gccgacaccc cggaccaggc
     3301 gcgcgacgcc gcggaggcgc tggtcgagcg gctgacggcg cgcgccgacc tgttcgatca
     3361 ggtgttcagc ccgggcatcg gcccgttctt cgaccgcaac gggctccttt acctcgaccg
     3421 cgacgagctg ttggaggtgc tcgacggcat cgccgactcc tcgcccctga tccagcgcct
     3481 gcgcgacgac accaccttgc gcgggctgtt cgcggtggtg ggcctggcga gcgaggacgc
     3541 cctcggcgac gccgaggccg ccgacaacct cgactggctg tacgggcgca tcgcgcgcgt
     3601 cgccgaggcg cgcctggacg gcgcgccggc gctgttgtcg tggcaggaga tcatgttcgg
     3661 gcgcgaggcc gagcccgagg acctgcgcac caccgtgatc gtgcgcccgg cccggatcga
     3721 ctacggcacg ctgctgcccg gggcggacgc gatcggcgag ctccgcggca tcgtgcgcga
     3781 ggcgggcctg acgcccgcgg cgggcgtgcg cgtatccttg accggcgggc cggcgctcga
     3841 gctcgacgag ctcgccaccg tccagcgcgg catcgcctta accggtgtgc tgtcgctggt
     3901 gctggtggtg gcgatcctgt gggccggcct gcgctcggcg cgcatggttg cggcgaccct
     3961 ggccaccttg gtgatcggcc tgatctggac cgccggcttc gcggcgctcg cggtcgggca
     4021 tctgaacctg atctcggtcg cgttcgcggt gctgttcgtg agtctcggcg tcgacttcat
     4081 gatccacctc gcgctcaggg tccaggaggc cgccgccggt gccgttcccg accggatctc
     4141 ggtcgccgcc gggacgcgcg cggtggcgag ctcgctcggc ttgtgcgcgc tgaccaccgc
     4201 ggtcggcttc tacgccttcc tgccgaccgc ctatcgcggc gtcgccgagc tcggcgtgat
     4261 cgccggcacc ggcatgatcg tcgcgctgct cgccagcctc accgtgctgc cggcgctgct
     4321 ggtgctgtgg ccggccaagg tgccggcgcg cagggccgtg gcgccgccgg ccgtgcgcct
     4381 gccgcgggtc tcggcaccgg cggtgcgcgg cggcgcgctg gcggggctgg tgctggcgct
     4441 ggccgccctg ccgtggctca gcttcgatgc caacccgctc aacctgcagg accccgaggc
     4501 cgagtcggtg cgcgcggtgc gccacctgat ggccgagggc gagcgcgacc tgtgggcggg
     4561 ggcgagcctg gccgccgacg ccggcgaggc tgagcgcctg gcggccgcgt tcgatgcgct
     4621 cggcgaggtc gaccgggcgg tgtggctcgg cgacctggtg ccgcccgacc aggaccagcg
     4681 gctcgacgtg atccaggaca tcgccttcgc cgccgagccg gacccgttcg cgacccccta
     4741 cccggcgcca agcgcggacg agcagcgggc ggcggtggcg gcgctcgccg gcgcgctcgg
     4801 caagctcggc gcgagcctgc cgctcggcga cggcggccgg gcgctcggcc gcgccctcgc
     4861 ccgtctcgag gcggcgcccg acgcggcgat cgccggggtc gatgcgggcc tggtcggcgc
     4921 cttgccggcg cggctgcagg cgctcgacga cgcgctcggc gccgagccgt tcggcgtcga
     4981 cgacctgccc gaggcgctgc gctcgcgcta tcgcggcacc gacggcagct tgcgcgtcga
     5041 tgtttacccg gccgaggacg tgatcgcgga caacggcgcg ctcgcccgct tcgtcgccgc
     5101 gctgcgcagc gtcgatccgg cggtcaccga cgacccggtg atcgtgttcg aggccggccg
     5161 ggtggtggtc gcctcgttcg tccaggcgct ggcctcggcg ctggtgctga tcttgctgct
     5221 gttgtacgtc ctgttccggc ggctggtcga tgtcctggtg gtgctggcgc cgctcgtgct
     5281 ggcgggtgcg atcaccggcg cggcgacggt cgtgctgggc atgtcgttca acttcgccaa
     5341 cgtcatcgtt ctgccgcttt tgctgggtat cggggtcgac agcggtatcc acatggtgca
     5401 tcgctatcgc atggagccgg atcggccggt gtggcggacc tcgaccgcgc gcgcggtggt
     5461 gttcagcgcg ctgaccacga tcgccggctt cggcagcctg gcattgtcac cgcatcccgg
     5521 cgccgccagc atgggcgcgc tgctgctgat cggcgtggtg atcacggtcg gctgcacgct
     5581 tggcgtgctg ccggcgctgc tgccgttcac ccgcccggcg ggcagggcgg ggtgaagcgg
     5641 ctgatcgccg tgctcggcct cgccgggctc gcgctcgcca ccggactgat cgtctggcag
     5701 ggcgcaggcg cggtcggctc ggccgtgctc gccgccggct ggggcctggc gatcgtgatc
     5761 gcggtcgaga tcctgccgct cggcatcgac gccctcgcct ggcgcacgct gatccccgcg
     5821 ggcgtgcgcc cgggcgtcgg cgtcgcgctg tgggcgcgct ggatccgcca atcggtcggc
     5881 caactgctgc cggtcgccca ggtcggcggc gacgtggtcg gcgcgcgcat gctgtatctg
     5941 cgcggcgtgc ccggcgaggt cgccggggca accgtggtgg tcgacctgac gctcggcgcg
     6001 gcgacccagc tcgtctacgc gctcaccggg cttgcgctgc tgtgggcgcg cccgggcggc
     6061 gacggcgtgg cggccggggt gctgatcggc ggcggcgtgc tggtgctcgg cctcgccggc
     6121 ttcgtgatcg cgcagcgcaa cggcctgttc cgattcctcg cccgccgcct gcacgcggtc
     6181 tcgggcggcc tgctggcgct ggtcggaagc gccgagcggc tcgaccacgc cgtgcgcgcg
     6241 gtctacgcgc ggccctggga cctggcgcgc aacgccgccc tgcagcttct ctcgtggacc
     6301 accggcacgc tcgaggtatg gctggcgctg cgcttcatcg gccacccggt ggggctggtc
     6361 gaggcctaca tcctgcagag cctgacgcgg atggtgcgca gcgccgcgtt cccggtgccg
     6421 ggcgggctcg gcgtccagga ggccgggctg atggtgctcg ccggcctgct cggcctcaac
     6481 cccgagatcg gcctggcgct gtcgctgatc aagcgcgtgc gcgagctggt gctcggcgtg
     6541 ccgggcctga tcgcctggca ggcggccgag ggcaatcatc tgctgcgccg gcggcgccgg
     6601 ctcgcggcgg aggcgcccga gcagggggcg tcgtagccgg gtgcgcgcga tcctgttcgc
     6661 cgccggcgtc ggccgccgcc tggcggcggt cgccgacggc ctgcccaagg ccttgctccg
     6721 tctcggcggc gcgagcctgc tcgagcggca cctgcgcatc ctcgcgcatt gcggcatcgg
     6781 cgaggtggtg gtggtcaccg gcttcggcgc cgaggcggtc gccggcgagg tcgcccggct
     6841 cgataccggc gcttcggtgc gcctggtcca caaccctgac taccgggccg gcagcatcct
     6901 cagccaatgg gcggcgcgcg cgacgctcgc cgccggcgag gcagtgctgc tgatggacgc
     6961 ggacgtgctc tacgaccagc gcctgatggc gcgcctgatc ggctccggcc atgacgactg
     7021 cttcctgctc gaccgcgaca tcgtgcccgg cgaggagccg gtcaagctgt gcgtgcgcgc
     7081 gggccagctg gtcgacttcc gcaagcggcc gagcgagccg cacgactggc acggcgagtc
     7141 ggtcggcttc ttcaagctcg gcccgggcac cgcgcgcgag ctggtcgagg ccacccagcg
     7201 ctacgtcgag gccggacgca ccgaggagta ttacgacgag gcgttgcgcg acctgctggt
     7261 ggccggcccg ccgggccggt tcggcttcga ggacgtcacc ggcctgccct ggatcgagat
     7321 cgacgtcccc gacgacctgg accgcgccga gcgcgaggtg ctgccgcggc tggagccgct
     7381 gccgccgcgg cccgaggccg ggcgatgagc cacgacacct gggcgcaccg catgatcgcg
     7441 ccggcggtcc ggccgctggc gcgcaccccg gtcaccccca accagctgac cgcggtccgg
     7501 ctggcgttcg gcatcgccgc ggcgggcgcc ttcgccgccg gcggcccggt ctggccgcag
     7561 gcgggcagcg cgctgtggct cgcctcgatg ctgttcgacc gcgccgacgg catcctcgcc
     7621 cggctgaccg gcaagaccag cgcctggggg cacaagttcg acctgttcgc cgatttcacc
     7681 agcaccacgc tggtgttcgc cggcatcggc atcggcctgc gcggcggcac gctcgggccg
     7741 tgggcgccgg cgctgggcgc ggtggcgggg ctgtcggtgg cggcgatctt cgccctggtc
     7801 taccgcatcc aaggcctgct gcccgccggt cgcagcgcgg tgccgagcgc ggccgggttc
     7861 gacgccgacg acgtgctgtt cgtggtggcg ccggtcgcct ggctcggctg gctgcagccg
     7921 ttcctggtca cggccgcggt cggcgcgccg ttggccgcgg cgctggcgct ggcgacactc
     7981 gcccagctgc gccgggctca gcggtcgaac accgtggtcg gcaacgagta gttgaggatc
     8041 aggaactcct cgccgggatt gtcgtcgtac aggccggcgt tcgacaggtg gccgaactgc
     8101 aggccgagcc tggagctgtc gtcgaaccgg taggcgagct cgaccgccga gcggaactcg
     8161 accgccccgc ccaggtcctt gccgttgccc tcgccgtagc cgccggcccc gatgctcggg
     8221 ctcagcacca ggcggcggcc gaagtagatg tcggtgaaga tgccggcata ggcgtagaag
     8281 ccggcgtcgt cggtgacgaa caggccgagc tgcgggcgga agacccacag ccgggtgttg
     8341 agcctcacct ggaggtcgag ctcggccgcg gccatgtcgt cgtcgaagtc gaaggcgccg
     8401 gcgccgacgg tgatgaaatc gggctcgcca tagcgctcct cggcggcggc gccgccggcg
     8461 cagacaattg gcgccgcgat caccatcatt cccagggcaa ggcgatgcct gaggccgcgc
     8521 gcggtcggtg ccgttggtgt catcccctgt ccttcatccc cgtggtcccg gcgcgcccgt
     8581 cccgtggtcg cgcaggcggt ccatgatctc gaccagcttg gcgcggatgc ccggctccat
     8641 cgacgagtgg ccggcgtcct ggaccacgtg gagctccgct tcgggccacg cccgcaccag
     8701 gtcgcacgcg gtctcgatcg ggc
//
LOCUS       DASWHU010000127         6768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2278875, whole genome shotgun sequence.
ACCESSION   DASWHU010000127 DASWHU010000000
VERSION     DASWHU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278875"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..731
                     /gene="efp"
                     /locus_tag="VGB88_04495"
     CDS             165..731
                     /gene="efp"
                     /locus_tag="VGB88_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9646733.1"
                     /translation="MKINGNAVRPGMVIEHKGALWRVVKTQATQPGKGGAYNQVELRN
                     LRDGSKLNERFRAAEDVERAYLEQKRYQYLYADDHLLTLMDTDTFEQIMVDVDLVEAG
                     RDFLQEGMEVEVEAFEGDVISVHLPDTVVMTVTATEPVVRGQTAASSFKPAVLENGVR
                     IMVPPHIETGTRVVVNTAEGTYAERAKE"
     gene            736..1563
                     /locus_tag="VGB88_04500"
     CDS             736..1563
                     /locus_tag="VGB88_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX9646734.1"
                     /translation="MTERLVHAGRVANRSAVVNVMVGAASRAARGLNRDFGEVQNLQV
                     SERGPDAFAAAAVARAGEVLAAELRRARRGFAYLAGGRSGGNGAGQARDSWLVEALDG
                     ADNYRHGLPWFAIVIAAREGGTITAAVIYEPLRDELFWAERGRGAYVNNQRLRVSARA
                     TLASALVATCITGGDDAESAGLRVAAAGRVAGLRETGAPALDLAAVAAGRFDGFWGAG
                     LSPATAEAGALLVREAGGLVEERGEAAGARRTLLAANPRLAAALGAMFGASDDAGHG"
     gene            1688..2842
                     /locus_tag="VGB88_04505"
     CDS             1688..2842
                     /locus_tag="VGB88_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9646735.1"
                     /translation="MVTGSRRQTAGARRACVIACAAAGLCLAGAARAQETVVVGAGLP
                     SVEVNEAALYGLGPGAGGPEARMVGVGPDGLPVYDPGLGAPRSRMLGMGPPATRSVPN
                     AESAIELRPPSRAAAAAAPPPPPVAAAPLRPSPPPAPAKAALPPAPEPAAAGGAPTPP
                     APPPVPEPAAGTKAAAAPPPLPPAPEMAKPAPPPKPQPAPEMAKAPARPEPETAVAAA
                     PPPPQPPPAAARPAPAATPPPTAAPPPPAPAPKAPEPAEQTMAALPPAETTPAEPPAS
                     AGVGVAGETVMVPFGPGAAELPDDSRPALDSVVSQLAADSAIRLQLKAYASAEDTAAS
                     AARRLSLSRALAVRSYLIDAGVSSTRIDVRALGSKYESGPPDRVDVITVR"
     gene            2951..4297
                     /locus_tag="VGB88_04510"
     CDS             2951..4297
                     /locus_tag="VGB88_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEX9646736.1"
                     /translation="MTRPRRFLFRMTVFLLLTVAIVVVLLEQLTHAFSANPPLNGLIL
                     GVLLLGIAYSFRQVLGLSPELRWIEGFRASKAAVSTQPAPRLLAPMAKMLADRQGKMM
                     LSTMATRSLLDSIAARLDESRDISRYMIGLLIFLGLLGTFWGLLDTVGSVSTVIKSLA
                     IADEDIVSTFTALKAGLDEPLRGMGTAFSSSLFGLSGSLVLGFLDLQSGQAQNQFYND
                     LEEWLSSVTRLSSGAGIAEGDQSVPVYVQALLEQTADSLEGLQRTLSRGEEDRQRATG
                     NIMQLTERLATLTDQMRAEQDLMVKLVESQMDMKPVLERLSEASERDTGIDEATRGHI
                     RNLDVYMMRLLEETVNGRSELASEIRSEIKLLARTIAASAGAAAARAGAQRQPAAAPA
                     PAGGTAPRPAAAPQPASAPQPAAPSAAESPASGGPRLSVRPTPTTIRRGGAGGGDS"
     gene            4330..5481
                     /locus_tag="VGB88_04515"
     CDS             4330..5481
                     /locus_tag="VGB88_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEX9646737.1"
                     /translation="MAGRARRASHGTDIWPGFVDALSTLLLVLIFLLVVFVLAEFFLG
                     RALSGRDEALARLNEQVMELADLLSLEKQANIDLRGDITQLSAQLQSSIADRDQLAGQ
                     VNTLLARQQELLAQIDTATADTADQETSVENLKLQLAAAMAMLEAAQATEQEAREKAN
                     AARTELSEQRRLSSTAERQVALLNQQIAQLRIQLAAVQQALGISETKVDEQKVQIADL
                     GKRLNIALASKVEELSRYRSEFFGRLREVLAGRSDVSIQGDRFVIQSGVLFDSGSADL
                     GGDGRQQIADLADLLIEISSEIPQDIAWILRVDGHTDMRPISTSQYRSNWELSAARAI
                     TVAEGLIARGVPPEHLAPAGFGEFHPLDPGDSEDAYRRNRRIELKLTER"
     gene            5525..6262
                     /locus_tag="VGB88_04520"
     CDS             5525..6262
                     /locus_tag="VGB88_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646738.1"
                     /translation="MTLIGHGSRFERRRRDRLRMAMLRLIAYATVIGAISYWAYSMGG
                     EHADQRARTLSQQMTALKEESEALRTEADAAVAARAAAVERAAQYQRRYEAEVPEGEV
                     RDIMESVRARIADGIAPARISHVVAAVENESSCEAEVTSRRFIIQTPISNGSNATVSF
                     ADNAIVVSGTGASARDGAGNAEAWFDAAQPIALDFTQPGGQASQAQGTLPLYHSVVID
                     DRDHRFTVQAADTRGFVTVTEQVCAYP"
BASE COUNT         1023 a         2427 c         2394 g          924 t
ORIGIN      
        1 acagccaagc ggtgggccgg tcggtccatg ccggtaacat gctcccgaaa gcgtccattg
       61 tccagatggg gtcccgggat cgcgctcctc cgttgccacg ggctgcgctc ggtgctagcg
      121 tccgcgccgc tttcgccaca accccggccc tgtccccgac gaacatgaag atcaacggca
      181 acgccgtgcg ccccggcatg gtgatcgagc acaagggcgc gctctggcgg gtggtcaaga
      241 cccaggcgac gcagcccggc aagggcggcg cctacaacca ggtcgagctc aggaacctgc
      301 gcgacggctc caagctgaac gagcgcttcc gcgccgccga ggatgtcgag cgcgcctatc
      361 tcgagcagaa gaggtatcag tacctgtatg ccgacgacca cctgctgacc ttgatggata
      421 cggatacatt cgagcagatc atggtcgatg tcgacctggt cgaggcgggc cgcgacttcc
      481 tgcaggaggg gatggaggtc gaggtcgagg cgttcgaggg cgacgtgatc tccgtgcacc
      541 tgccggatac cgtggtgatg acggtgaccg cgaccgagcc ggtggtcagg ggccagaccg
      601 cggcctcgag cttcaagccg gcggtgctgg aaaacggcgt gcggatcatg gtgccgcccc
      661 acatcgagac cggcacccgg gtggtggtca acaccgccga aggcacctac gccgaacgcg
      721 ccaaggagtg accgcgtgac tgagcgtctg gtccatgcgg ggcgggtggc caaccggtcc
      781 gcggtggtca atgtcatggt cggggccgcg agccgggcgg cgcgcgggct caatcgcgat
      841 ttcggcgagg tgcagaacct gcaggtgagc gagcgcggcc cggacgcctt cgccgccgcc
      901 gcggtcgccc gggcgggcga ggtgctggcg gcggagctgc gccgcgcgcg ccgcggcttc
      961 gcctatctcg cgggcgggcg cagcggcggc aacggcgccg gccaggcgcg cgattcctgg
     1021 ctggtcgagg cgctcgacgg cgccgacaac tatcgccacg gcctgccgtg gttcgcgatc
     1081 gtgatcgccg cccgcgaggg cggcacgatc accgccgcgg tgatctacga gccgctgcgc
     1141 gacgagctgt tctgggccga gcgcgggcgc ggcgcctacg tcaataacca gcggctcagg
     1201 gtctccgcgc gcgcgacgct cgcctcggca ctggtcgcga cgtgtatcac cgggggcgac
     1261 gacgccgagt ccgcgggcct gcgcgttgcg gcggccggcc gggttgccgg gctgcgcgag
     1321 accggcgcgc cggcgctcga cctggccgcc gtcgccgccg ggcgcttcga cggcttctgg
     1381 ggcgcgggct tgtcgccggc gaccgccgag gcgggcgcgc tgctggtgcg cgaggccggc
     1441 ggcctggtcg aggaacgcgg cgaggctgcc ggcgcgcggc gcacgctgct cgccgccaac
     1501 ccgcgcctcg ccgccgcgct cggcgccatg tttggcgcgt ccgacgatgc cgggcacggt
     1561 tgagagcgag gccgctaatg ctgttgttca cgagggcgcg gctggctatt gtgctcggcg
     1621 ctgatttcgg cgctgggtgg tgccgctgcg ggcgcggacg aaaggattgg acgacggcga
     1681 caggaccatg gtgacgggca gccggcggca gacggccggc gcgcgccgcg cgtgcgtgat
     1741 cgcctgcgca gcggcagggc tctgccttgc cggcgcggcg cgggcgcagg agacggtggt
     1801 ggtcggcgcc ggcctgccgt cggtcgaggt caacgaggcc gcactgtacg ggctcggccc
     1861 gggtgccggc gggccggagg cccggatggt tggggtcggc cccgacggcc tgccggtcta
     1921 cgacccgggg ctcggcgcgc cgcgctcgcg gatgctcggc atgggcccgc cggcgacgcg
     1981 atcggtgccg aacgcggagt ccgcgatcga gctgcgcccg ccgtcgcgcg cggcagccgc
     2041 cgcggcgccg ccgccaccgc cggtcgcggc ggccccgttg cgcccgtcgc cgccaccggc
     2101 gcccgccaag gccgcactgc cgccggcgcc ggagccggcc gccgccggcg gggcaccgac
     2161 cccgcccgcg ccgccgccgg tgcccgagcc cgccgccggc accaaggccg ccgcggcccc
     2221 gccgcccttg ccgccggcgc cggagatggc caagcccgcg ccgccgccga aaccgcaacc
     2281 cgcgcccgag atggcgaagg cgccggcacg gccggaaccg gaaacggccg tggctgcagc
     2341 gccgccgccg ccgcaaccgc cgccggcagc cgcgcgcccg gccccggccg caactccgcc
     2401 gccgaccgca gcgccgccgc cgcccgcgcc cgcgccgaag gcgcccgagc ccgctgagca
     2461 aacgatggcc gcgctgcctc cggccgagac gacgccggcc gagcccccgg ccagcgccgg
     2521 tgtcggcgtc gccggcgaga cggtcatggt cccgttcggg ccgggcgcgg ccgagcttcc
     2581 ggacgactcg cggccggcgc tcgattccgt ggtttcccag cttgccgcgg actcggcgat
     2641 caggctgcag ctcaaggcct acgcctcggc ggaggacacg gcggcgagcg ccgcccgcag
     2701 gctgtcgctg tcgcgggcgc tcgcggtccg ctcctacctg atcgacgccg gggtcagctc
     2761 gacgcgcatc gacgtgcgcg cgctcgggtc taaatacgaa agcggcccgc ccgaccgagt
     2821 ggacgtcatt accgttcgct aggtgcgggc gccggcgctg gcgccgagcg ccggacatgc
     2881 ccacatgcgc gggggtgctt gggctggcag cgttgcggga ggccggcagg cgcgaaggga
     2941 tgacacttag atgaccaggc cgaggcgctt cctgtttcgc atgacggtgt tcctgctgct
     3001 caccgtcgcg atcgtggtgg tgctgctcga gcagctgacc cacgcgttct cggccaaccc
     3061 gccgctgaac ggcctgatcc tcggcgtgct cttgctgggc atcgcctaca gcttccgcca
     3121 ggtgctcggg ctcagcccgg agctgcgctg gatcgagggc ttccgcgcca gcaaggccgc
     3181 ggtctcgacc cagcccgcgc ccaggctgct ggcgccgatg gcgaagatgc tcgccgaccg
     3241 ccagggcaag atgatgctgt cgacgatggc gacgcgctcg ctgctcgaca gcattgccgc
     3301 gcggctcgac gagtcgcgcg acatctcgcg ctacatgatc gggctgctga tcttcctcgg
     3361 cctgctcggc accttctggg gcctgctgga caccgtcggc tcggtcagca cggtgatcaa
     3421 gagcctggcg atcgccgacg aggacatcgt cagcaccttc accgcgctca aggcggggct
     3481 cgacgagccg ctgcgcggca tgggcaccgc gttctcctcg tcgctgttcg gcctgtccgg
     3541 atcgctggtg ctcggtttcc tcgacctgca gtcgggccag gcgcagaacc agttctacaa
     3601 cgacctcgag gaatggctgt cgagcgtcac ccggctgtcg agcggcgccg gcatcgccga
     3661 gggcgaccag tcggtgccgg tctacgtcca ggcgctgctc gagcagaccg cggactcgct
     3721 cgaaggcctg cagcgcacgc tgtcgcgcgg cgaggaggac cgccagcgcg ccaccggcaa
     3781 catcatgcag ctgaccgagc ggctggcgac gctgaccgac cagatgcgcg ccgagcagga
     3841 cctgatggtc aagctggtcg agagccagat ggacatgaag ccggtgctcg agcggctgtc
     3901 ggaagcgagc gagcgggata ccggcatcga cgaggcgacc cgcggccaca tccgcaacct
     3961 cgacgtctac atgatgcgcc tgctcgagga gaccgtgaac gggcgcagcg agctcgcctc
     4021 ggagattcgc agcgagatca agctgctcgc ccgcaccatc gccgccagcg ccggggccgc
     4081 ggctgcgcgc gcgggcgccc agcgccagcc cgccgcggcg ccggcaccgg cgggcggcac
     4141 cgcgccgaga cccgcggccg cgccgcagcc cgcatcggcg ccgcagccgg cggcgccgtc
     4201 cgcggccgag tcgcccgcgt ccggtgggcc caggctttcg gtgcgcccga ccccgaccac
     4261 catccggcgc ggcggcgccg gcggcggcga ctcgtgagcg gggctcgcgc accgtcggga
     4321 ggctaggcga tggccggccg cgcgcgccgc gccagtcacg ggaccgacat ctggccgggc
     4381 ttcgtcgacg ccctgtcgac cctgctgctg gtcctcatct tcctgctggt ggtgttcgtg
     4441 ctggccgagt tcttcctcgg ccgcgcgctg tccgggcgcg acgaggcgct ggcgcggctg
     4501 aacgagcagg tgatggagct cgccgacctg ctgtcgctcg agaagcaggc caatatcgac
     4561 ctgcgcggcg acatcaccca gctgtcggcg cagctgcaat cctcgatcgc cgatcgcgac
     4621 cagctcgccg gccaggtcaa caccctgctc gcgcgccagc aggagctgtt ggcccagatc
     4681 gataccgcga ccgccgatac cgccgaccag gagacctcgg tcgagaacct caagctgcag
     4741 ctcgccgcgg cgatggcgat gctcgaggcg gcccaggcca ccgagcagga ggcgcgcgag
     4801 aaggccaacg ccgcgcgcac cgagctgagc gagcagcgcc ggctgtcatc gaccgccgaa
     4861 cgccaggtgg cgctgctcaa ccagcagatc gcccagctgc gcatccagct cgccgccgtg
     4921 cagcaggcgc tcggcatcag cgagaccaag gtcgacgagc agaaggtgca gatcgccgat
     4981 ctcggcaagc gcctcaacat cgcgctggcg agcaaggtcg aggagctgtc gcgctatcgc
     5041 tcggagttct tcggccggct gcgcgaggtg ctcgccgggc gcagcgacgt cagcatccag
     5101 ggcgaccgct tcgtcatcca gtccggggtg ctgttcgact cgggctcggc cgatctcggc
     5161 ggcgacggcc gccagcagat cgcggacctg gccgacctcc tgatcgagat ctcgtccgag
     5221 attccccagg acatcgcctg gatcctgcgg gtcgacggcc acaccgacat gcgcccgatc
     5281 agcacctccc agtaccggtc gaactgggag ctgtcggcgg cgcgggcgat caccgtcgcc
     5341 gagggcctga tcgcgcgcgg cgtgccgccc gagcacctgg ccccggccgg gttcggcgag
     5401 ttccacccgc tcgatcccgg cgacagcgag gatgcctacc ggcgcaaccg gcgcatcgag
     5461 ctcaagctga ccgagcgcta gcagtcgcgg gctggagctt gccgcgggcc ggggactcgc
     5521 tgcgatgacg ctgattggcc acggaagtcg attcgaacgc cgccggcgcg accgcttgcg
     5581 catggcgatg ctgcgcttga tcgcctatgc cacggtaatc ggcgcgatct cgtactgggc
     5641 ctacagcatg ggcggcgagc acgccgacca gcgcgcccgc acgctgagcc agcagatgac
     5701 ggcgcttaag gaggagagcg aggcgctgcg caccgaggcc gatgccgcgg tcgccgcccg
     5761 ggcggcggcg gtggagcgcg ccgcgcagta ccagcgccgc tacgaggccg aggtgcccga
     5821 aggcgaggtg cgcgacatca tggagtcggt gcgcgcgcgc atcgccgacg gcatcgcgcc
     5881 ggcacgcatc tcccacgtgg tcgcggcggt cgagaacgag agcagctgcg aggccgaggt
     5941 gaccagccgc aggttcatca tccagacgcc gatctcgaac ggcagcaacg ccacggtgtc
     6001 gttcgccgac aacgcgatcg tggtctccgg caccggcgcg tccgcgcgcg acggcgccgg
     6061 caacgcggaa gcctggttcg atgccgccca gccgatcgcc cttgacttca cccagcccgg
     6121 cggccaggcg agccaggccc agggcacctt gccgctgtac cactcggtgg tgatcgacga
     6181 ccgcgaccac aggttcacgg tccaggccgc cgacacgcgc ggcttcgtca ccgtcaccga
     6241 gcaggtctgc gcctacccgt gacaaaacaa ttactattaa gacttatgaa tatgctttac
     6301 accctcaccg caatttgcca tacggcaaga tcgccgtatt tcgaaccgca gagtcaccag
     6361 actgcgagtt ctcacaccga cgtcgctgct accagaaata gcgggctaga gaaccggtcg
     6421 gatacgacct aaactttcct ccacaaaatc ccatcgccaa ccccaatgag ggccaacata
     6481 gtgccgaaac aaacgaataa tcttaatccc tttcgagatt ccagaaccat ctcgttaaaa
     6541 cggcaggaac tctaagatgg tcaacacaat ccaagcggat gctagtccag agaagcggct
     6601 attcatctac ttgatcacga gagacatctc gttgatcgat gctttccttg acctcatcga
     6661 caattctgta aacgctgcgc ttgtgccact cgccgaccgc cttaggaccg ctgacgatta
     6721 cgaacgtttg ttgtagctta tgcacgacgc tccggccgca cgacatgt
//
LOCUS       DASWHU010000128         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2281014, whole genome shotgun sequence.
ACCESSION   DASWHU010000128 DASWHU010000000
VERSION     DASWHU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281014"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VGB88_04525"
     CDS             complement(<1..489)
                     /locus_tag="VGB88_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9646739.1"
                     /translation="MRMPDPDRAVIEGRARLVERLRDIVPGEGVIAHADELRAFETDG
                     LTAYRALPMVVVLPETTAQVSKVLALCKQAGAKVVPRGAGTGLSGGALPLADGVTLGL
                     SKFNRILDIDLDNRCAVVQPGVTNLAISQAVAGAGFYYAPDPSSQIACSIGGNVAENS
                     GGV"
     gene            complement(618..743)
                     /gene="ykgO"
                     /locus_tag="VGB88_04530"
     CDS             complement(618..743)
                     /gene="ykgO"
                     /locus_tag="VGB88_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX9646740.1"
                     /translation="MKVRMSLKTAKRRHKDCRVVRRKGRVYVINKTNPRFKARQG"
     gene            complement(845..>1850)
                     /locus_tag="VGB88_04535"
     CDS             complement(845..>1850)
                     /locus_tag="VGB88_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9646741.1"
                     /translation="GLKEHSVRILVKSGIGAAGAFAAQIVNGPARARLAPMLGRFHAW
                     WEGEDWDPEAYPAPAEAAADEPAAAGLTVESSQSTPWSPARRQVVEGLWGAGFSAPGD
                     DAQVLALVAPLGLDATRSAIEIGAGLGGAARAIARETGAHVTGLEASPDLVEAAMALS
                     AKAGLAKRAPVTLFAPPHLDLADGALDAIVSRAALFALAAKDELFADCHRVLKPAGQL
                     LVTDYLLARDGDSSTALDDWIANELPQPRPWSVARTLACLEELGFELRASEDVGAETR
                     KLALAGWDRFVRMLSPGAVAPDVARAIVGELELWRRRLELLAAGALCCRRIHAVKRQA
                     "
BASE COUNT          238 a          702 c          667 g          243 t
ORIGIN      
        1 cacgccgccc gagttctcgg cgacgttgcc gccgatcgag cacgcgatct ggctcgacgg
       61 gtctggggcg taatagaacc cggcgcccgc caccgcctgg ctgatggcga ggttggtcac
      121 ccccggctgg accaccgcgc agcggttgtc gaggtcgatg tcgaggatgc ggttgaactt
      181 gctcaggccc agggtgacgc cgtcggcgag cggcagcgcg ccgcccgaca gcccggtgcc
      241 ggcgccgcgc ggcaccacct tggcgccggc ctgcttgcac agcgccagca ccttcgagac
      301 ctgcgccgtg gtctcgggca gcaccaccac catcggcagc gcgcggtacg cggtcagccc
      361 gtcggtctcg aacgcgcgca gctcgtccgc gtgcgcaatc acgccctcgc cggggacgat
      421 gtcgcgcagg cgctcgacca gccgggcgcg gccctcgatc acggcacggt ccgggtccgg
      481 catgcgcatg gcacgggtcc tcgagctggt ggcgcaagcg cgcgccggcg cgccgccctt
      541 ggcggcggcc cgctcgcgat ggcctcgatt gtgggaccgc gcgccggccc ggtcaatccg
      601 gcgcggcgcg cacgcgccta gccctggcgc gccttgaacc gcgggttggt cttgttgatc
      661 acgtagaccc ggcccttgcg gcgcaccacc cggcagtcct tgtgccggcg cttggcggtc
      721 ttcagcgaca tgcgcacctt catggccggt cctcgtccga tacgcggtgc cgcgccaggt
      781 ccggcgcggc ggcgcggacc ttaggggtgg cgccccccgc tgtcaatccg cgccgctgcc
      841 cgcgtcatgc ctggcgcttg accgcatgga tgcgccggca gcacagcgcg ccggccgcga
      901 gcagctcgag gcggcggcgc cacagctcga gctcgccgac gatcgcgcgg gcgacatcag
      961 gcgccaccgc gccggggctg agcatgcgca cgaaccggtc ccagccggcg agtgccagct
     1021 ttctcgtctc ggcgccgaca tcctcgctcg cgcgcagctc gaagcccagc tcctcgaggc
     1081 aggcgagcgt gcgcgcgacg ctccacggcc ggggctgcgg cagctcgttg gcgatccagt
     1141 cgtcgagcgc cgtcgagctg tcgccgtcgc gcgcgagcag gtagtccgta accaagagct
     1201 ggccggcggg cttgagcacc cgatggcagt ccgcgaacag ctcgtccttg gcggcgagcg
     1261 cgaacaacgc cgcgcgcgag acgatcgcgt cgagcgcgcc gtcggcgagg tcgagatgcg
     1321 gcggcgcgaa cagagtgacc ggcgcgcgct tggcgagccc cgccttggcc gacagggcca
     1381 tggcggcctc aaccaggtcc gggctcgcct cgaggccggt gacatgcgcc ccggtctcgc
     1441 gcgcgatcgc gcgcgccgcg ccgccaaggc cagcgccgat ctcgatcgcg ctccgcgtcg
     1501 catcaaggcc gagcggcgca accagcgcca gcacctgcgc atcgtccccg ggcgcagaga
     1561 agcctgcgcc ccacaggccc tcgaccactt ggcggcgcgc cggcgaccag ggtgtggatt
     1621 gggacgactc gacggttaag cccgctgccg ccggctcgtc ggcggcggcc tcggcgggcg
     1681 cggggtaagc ctcggggtcc cagtcctccc cctcccacca ggcgtgaaag cggccgagca
     1741 tcggcgccag gcgtgcgcgc gccgggccgt tgacgatctg cgcggcgaaa gcgccggcgg
     1801 cgccgatccc ggatttcacc aagatgcgca cgctgtgttc cttcagccct
//
LOCUS       DASWHU010000129        10656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_798768, whole genome shotgun sequence.
ACCESSION   DASWHU010000129 DASWHU010000000
VERSION     DASWHU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798768"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..2205)
                     /locus_tag="VGB88_04540"
     CDS             complement(136..2205)
                     /locus_tag="VGB88_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646742.1"
                     /translation="MGAITTVTFDPALPAWALAALGTAALALLGWGAVRRAHGLAWRA
                     LVMAALWLALLGPSVTREERRALADVVALVTDHTASQEIGARMAAADEAARAIETRLA
                     ALGGLEVRTVTVDGRGGADGTRLFTARDRLLADVPAERVAGTIMITDGQVHDAGEAKA
                     EATGPLHVLLTGARAERDRRLAVVNAPSYGIVGDKVALAVRVEDERATAAEVVLEVRG
                     DGRRLRRAPARIGERTELEVPVAHAGVTAVEIEVEPAAGELTTANNKVVVLINGVRDR
                     LRVMLVSGEPNPGLRTWRNLLKADPSVDLVHFTILRPPNKQDMTPVRELSLIPFPTNE
                     LFAANLAEFDLVIFDNYHRRGILPMVYLANVVDYVMAGGAVLDAAGPSFASPLSLAGT
                     PVGTILPGQPTGRVFREPFTPQLTPEGRRHPVTATLPGSGATAGEAGAPQWGRWFRQV
                     DVTQNGGVALMSGIGERPLLVLDRIGDGRVAQLLSDQSWLWARGYEGGGPQSDLLRRL
                     VHWLMKEPDLEEESLVARVVDDRVEVTRHSLNRIEGEVAVTGPDGTVSTLALEDQGDG
                     RATATVTADAQGLYRLDQDGHTAIAVVGAADALEMADARATDAVLRGIAEAGGGSVTW
                     LADSGVPDIRRVRPGRASAGRGWIGVVQREGYQVTGLDQMPLLPAWLMLTLGLGGLML
                     AWRAEGR"
     gene            complement(2216..4996)
                     /locus_tag="VGB88_04545"
     CDS             complement(2216..4996)
                     /locus_tag="VGB88_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX9646743.1"
                     /translation="MTGLGALSFAVPWALAALAALPVIWWLLRLNPPAPRRIAFPAIR
                     LLFGLKPAEETPTHTPLWLLLLRLALAALVIVAIAQPLLNAQRTVAVDGPVVLVIDDG
                     WAAAPGWEKRLDVARTLLEEAQRRHRPAAVAGTAPTTEGAGGLGGFAATGLVEAGEAQ
                     SFVRVLAPKPWPAARAAAAEALAASLPALGDAEVVWLSDGLADAEPGATERFAERLAD
                     IGPLTVYADAPAERAKLLLAPDAAAARMTVRVVRAAARAGAAAYLLARDGDGRVLGRQ
                     AVAFADGAVEAEVALELPGELRNEVARIELEGESSAGAVVLVDERWRRRPVGLVSGGA
                     FEQSQPFLSDLYYLDRALSPFAEVQRGDIDGLLASPLSVLMLSDVGQLPDAIRQRLAA
                     WIESGGLLVRFAGPRLAERVDDLVPVMLRAGGGRALGGALSWDKPMRLSPFDAESPFA
                     GLAIPDDVVVKRQVLAEPSLGLAERSWARLEDGTPLVTAAPRGRGWLVLFHTTANTDW
                     SSLALSGLFVDMLQRIVSLSEGVAGPEGEERLQPLTGLDGFGRLGDPLPEAAPVAARE
                     LGLTRAGPRHPPGEYGNRMQRRALNLGTPGLAHAAFGELAGAATMQGYGALRTVDLQP
                     PLLAAAFVIALADLVIALAFRGLLPVPAAARGAAVIVAAALAAAAVAPPGEAQEPGDE
                     ALAALTQDTHLAYVRTGDAEVDGTSLTGLSGLTTILQARTAIEAGEPVAVDPESDELA
                     FFPLIYWPMTDGQGPLSDGARAHVAEYLRLGGMILFDTRDQSPVERLGGDRPHNARLA
                     TLLSGLNVPPLLPVPQDHALTRSFYLIDHFPGRWDGGQVWVERYEGDVNDGVSSIVIG
                     GNDYAAAWALDGDGYPLYPVVPGGNRQRELAFRFGVNLVMYALTGNYKADQVHIPAIL
                     SRLGRARPDLTD"
     gene            complement(4993..5886)
                     /locus_tag="VGB88_04550"
     CDS             complement(4993..5886)
                     /locus_tag="VGB88_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9646744.1"
                     /translation="MAGPASAVQARERAEQAAAALPALLVAARRVAATVLQGVHGRRQ
                     AGPGETFWQFRRYQPGDAASLIDWRQSAKSVPLYVREQEWEASASVWLWRDASPSMDW
                     RSPGIDQTKAARATLLLLALAALLLRGGEQVALLGHGRRPLRGTASLERLAQTLMHAP
                     IGVAGLPGRDYLPRHAQVVMIGDFLDPVDDLHGAVNWLTGTGATGHLLQVLDPAEESL
                     PFAGRVRFDGTEGEGSMLIGRVEAVRAAYRDRLAAQRAALAALARSSGWTFLSHRTDR
                     PPETALLALYLALSGALGRKR"
     gene            complement(5907..6926)
                     /locus_tag="VGB88_04555"
     CDS             complement(5907..6926)
                     /locus_tag="VGB88_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9646745.1"
                     /translation="MTDPAPAAIKSVTPPAPLVAGVESLAGKMAEVRRQVGKRIFGQH
                     GVVDLTLITLLSGGHALVVGVPGLAKTRLTETLGQVIGLSEKRIQFTPDLMPADIIGS
                     EVLEESETGRRSFRFINGPVFCQLLMADEINRASPRTQSALLQAMQENEVSVAGIRHK
                     LPHPFHVLATQNPLEQEGTYPLPEAQLDRFLMQIDIDYPDADDERRMILATTGIEESE
                     PRQAMTADELIAAQRIVREVPVGDSVVDAILTLVRAGRPGNSDIELVRDSVSWGPGPR
                     ASQALMLASRARAILDGRLSPSIDDVLALAGPVLRHRMDITFSARADGVTIDRVIGAM
                     CQPLL"
     gene            6980..7543
                     /locus_tag="VGB88_04560"
     CDS             6980..7543
                     /locus_tag="VGB88_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX9646746.1"
                     /translation="MAGTPGDDPKQSATDPPRPAGLAPDGPHQRGDVDIRIGRDGRWF
                     YHGSPIGRKPLVKLFASVLRRDEAGDYWLQTPVEKARIRVDDAPFVAVALAVEGEGRD
                     QRLVFRSSLDDVVCADADHPIRIAVDARTGEPAPYVTVRPGLEALIARSVFYQLVDLA
                     DQCPESHILGVWSAGIWFPLGSAGEAT"
     gene            7540..8154
                     /locus_tag="VGB88_04565"
     CDS             7540..8154
                     /locus_tag="VGB88_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9646747.1"
                     /translation="MTRDLVMMKVGKRTAGRIIPASLAHGEPSASAESATIEAAVLVP
                     LIDRPDGMTVLLTQRSEHLTDHAGQVSFPGGRVEPGDRGPADTALREAEEEIGLAPPR
                     VEVVGCLDICVTGTGFRVVPVVGLIAAPFDIARDLTLDAFEVAEAFEVPLAFVLDESH
                     HERRTAIARGARREFYVLPYQDRFIWGATARMLVNLHEVLRGRS"
     gene            8151..8426
                     /locus_tag="VGB88_04570"
     CDS             8151..8426
                     /locus_tag="VGB88_04570"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEX9646748.1"
                     /translation="MRTLLTRILPLLLPVLVYLGWWYLARRRALARGETGPALSEAPW
                     TWLFVAGFALLVMTLVVLGLTWGEEPGGVYVPPRYEGGKIVPGHIER"
     gene            8437..9678
                     /locus_tag="VGB88_04575"
     CDS             8437..9678
                     /locus_tag="VGB88_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9646749.1"
                     /translation="MTPTGQIALQPWMQAPETAEVLAALQARGTTVRFVGGCVRDALL
                     GKEVGDIDIATPDPPETVLALIESAGLKAVPTGLAHGTVTAIAGGVPFEVTTLRRDVE
                     TYGRHARVAFIDDWTDDAARRDFTINAMFLDPDGTLYDPFGGRADLAAGHVRFVGEAR
                     RRIDEDVLRLLRFFRFHAWYGRPPADDEALAACRELAPRLETLSGERLQAELFKLLAA
                     PAPLPALELMADAGVMPHLLPNARAGWQARLAALVGLEDAPANGDAVRRLAAIVAAPT
                     EALAARLRLSRADGERLAALAGAAELPAPAMDARAVRAMLYRLGPELFRDLALLAWSE
                     AGGGEDDGFARLLAAAADWPSPELPVRGRDVLARGIPKGTAVGALLAEVERWWVAGDF
                     RAGRAEALAYLDQLIAGQGRG"
     gene            9671..10654
                     /locus_tag="VGB88_04580"
     CDS             9671..10654
                     /locus_tag="VGB88_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018880841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEX9646750.1"
                     /translation="MAEPACWALTTGEAGAVSQAAGLAEAVGLPWVAKRIELRRPWRW
                     LPGHLCPVALAGLGPGGDRLAPPWPELLISCGRRAVAAAIAIRRRAAGGTFAVHLQHP
                     HVPTHLFDMVVPPRHDRLAGANVHATRVALHGITAARLAEAGAHFRSRFAHLPRPLVA
                     VLVGGDSGSYRLTPETARDLADRLAALAREAGAGLIVTVSRRTGAGNTAVLRARLGAA
                     GAEVWDGSGENPYLGMLALADHIVVTADSVSMVSEACATGKPVHVFPLPGGSARFRRF
                     HDALSAEGVTRPFSGRLDSWSYPPIVEAEAVGAIVRAKLETRRAPAGAAGG"
BASE COUNT         1356 a         3999 c         3926 g         1375 t
ORIGIN      
        1 gaagcgttcg agacccaggt ccgcgagcca gtgccggagc tgcgtcacag cgcaccccgc
       61 gaccgtgttg tactctgtgc gatcaagtag accccgcccg cgcgccgggg gcaagggcgc
      121 gtgcggcgcc gcggctcagc gcccctcggc gcgccaggcg agcatcaggc cgccgaggcc
      181 gagggtcagc atcagccacg cgggcaacaa cggcatctgg tcgaggccgg tgacctggta
      241 gccctcgcgc tggaccacgc cgatccagcc gcgccccgcg ctcgcgcggc ccgggcgcac
      301 gcggcggatg tcgggcacgc ccgagtcggc gagccaggtg acgctgccgc cgccggcctc
      361 ggcgattccg cgcaggacgg catcggtcgc gcgcgcgtcc gccatctcga gcgcatcggc
      421 cgcgccgacc accgcgatcg ccgtgtgccc gtcctgatcg aggcggtaga ggccctgcgc
      481 gtccgcggtc acggtcgcgg tggcgcggcc gtcgccctgg tcctcgagcg cgagcgtcga
      541 gaccgtgccg tcagggccgg tcaccgccac ttcgccctcg atccggttca ggctgtggcg
      601 ggtgacctcg acgcggtcgt cgaccaccct ggcgactagg ctctcctcct cgaggtcggg
      661 ctccttcatt agccaatgca ccaggcggcg cagcaggtcg gactgcggcc cgccgccctc
      721 gtagccgcgc gcccacagcc atgattggtc ggacaacagt tgcgccaccc ggccgtcgcc
      781 gatgcggtcg agcaccagca gcggccgctc gccgatgccg ctcatcagcg ccaccccgcc
      841 gttctgggtg acgtcgacct ggcggaacca gcgcccccat tgcggtgcgc cggcctcgcc
      901 ggcggtggcg ccgctgccgg gaagcgtggc ggtgaccgga tggcgacggc cctcgggcgt
      961 gagctgcggg gtgaacggct cgcggaacac gcgcccggtc ggctggccgg gcaggatggt
     1021 gccgaccggc gtgccggcaa ggcttagcgg cgaggcgaac gacggcccgg ccgcgtccag
     1081 caccgcgccg cccgccatca cgtagtcgac gacgttggcg agatagacca tcggcaggat
     1141 gccgcgccgg tggtagttgt cgaagatcac caggtcgaac tcggccaggt tcgccgcgaa
     1201 cagctcgttg gtcgggaacg ggatcagcga cagctcgcgc accggcgtca tgtcctgctt
     1261 gttgggcggg cgcaggatgg tgaagtggac caggtcgacc gaggggtcgg ccttgagcag
     1321 gttgcgccaa gtccgcaggc ccgggttcgg ctcgcccgag accagcatca cccgcagcct
     1381 gtcgcgcacg ccgttgatca gcaccaccac cttgttgttg gccgtggtca gctcgcccgc
     1441 tgccggctcg acctcgatct cgaccgcggt caccccggca tgggcgaccg gcacctcgag
     1501 ctcggtgcgc tcgccgatgc gcgccggcgc gcggcgcagg cggcggccgt cgccgcgcac
     1561 ctcgagcacc acctcggccg cggtcgcgcg ctcgtcctcg acgcggaccg cgagcgcgac
     1621 cttgtcgccg acgatgccgt agctcggcgc attgaccacg gcgagccggc ggtcgcgctc
     1681 ggcccgcgcc ccggtcagca gcacgtgcag cgggccggtg gcctcggcct tggcctcgcc
     1741 ggcgtcgtgg acctggccgt cggtgatcat gatcgtgccg gcgacgcgct cggccggcac
     1801 gtcggcgagc aggcggtcgc gggcggtgaa caggcgggtg ccgtcggcgc cgccgcggcc
     1861 gtcgacggtg acggtgcgga cctcgaggcc gccgagggcg gcgagccgcg tctcgattgc
     1921 gcgcgccgcc tcgtcggccg ccgccattcg cgcgccgatc tcctggctcg cggtgtggtc
     1981 ggtgaccagc gccaccacgt cggcgagcgc gcgccgctcc tcgcgcgtca ccgacggccc
     2041 gagcagcgcc agccacagcg ccgccatcac cagcgcccgc caggcgaggc cgtgcgcccg
     2101 gcgcaccgcg ccccagccga ggagcgcgag tgcagccgtc ccgagcgccg cgagcgccca
     2161 cgctggcagc gccgggtcga aggtgacggt ggtgatcgcg cccatcgccg cggcgctagt
     2221 cggtgaggtc cgggcgcgcg cgcccgagcc ggctcaggat cgccgggatg tgcacctggt
     2281 cggccttgta gttgccggtc agcgcgtaca tcaccaggtt gacgccgaac cggaacgcaa
     2341 gctcgcgctg gcggttgccg cccggcacca ccgggtacag cgggtagccg tcgccgtcga
     2401 gcgcccaggc ggcggcataa tcgttgccgc cgatcacgat cgaggacacg ccgtcgttga
     2461 cgtcgccctc gtagcgctcg acccacacct ggccgccgtc ccagcggccg ggaaagtggt
     2521 cgatcaggta gaacgagcgg gtcagcgcgt ggtcctgcgg caccggcagc agcggcggca
     2581 cgttgaggcc gctcaagagc gtggcgaggc gcgcattgtg cgggcggtcc ccgcccaggc
     2641 gctcgaccgg cgactggtcg cgggtgtcga acaggatcat gccgccaagg cgcagatact
     2701 cggcgacgtg cgcccgcgcg ccgtccgaca gcggtccctg gccgtcggtc atcggccagt
     2761 agatcagggg aaagaacgcc aactcgtcgg actcgggatc gaccgccacc ggctcgcccg
     2821 cctcgatcgc ggtgcgcgcc tgcaggatcg tggtcaggcc ggacaggccg gtcaggctgg
     2881 tgccgtcgac ctcggcatcg ccggtgcgca cgtaagcgag gtgggtgtcc tgggtcagcg
     2941 ccgcgagcgc ctcgtcgccg ggttcctgcg cctcgcccgg gggcgcgacg gccgccgccg
     3001 ccagcgccgc ggccacgatc acggccgcgc cgcgcgccgc tgccggaacc ggcagcaggc
     3061 cgcggaaggc gagcgcgatg accaggtcgg cgagcgcgat gacgaacgcc gcggcgagca
     3121 gcggcggctg caggtcgacc gtgcgcagcg cgccgtagcc ctgcatcgtc gccgctcccg
     3181 ccagctcgcc gaacgccgca tgcgcgagtc ccggcgtgcc gagattgagc gcccggcgct
     3241 gcatccggtt gccgtactcg ccgggcgggt gccgcggccc ggcgcgcgtc agcccaagct
     3301 cgcgcgccgc caccggcgcc gcttccggca acggatcgcc gagccggccg aagccgtcga
     3361 ggccagtcag cggctgcagc cgctcctcgc cctcgggccc ggcgacgccc tcgctcaggc
     3421 tgacgatccg ctgcagcatg tcgacgaaca gcccggacag ggcgagcgag gaccagtcgg
     3481 tgttggcggt ggtgtggaac agcaccagcc agccacggcc gcgcggcgcg gcggtgacca
     3541 gcggcgtgcc gtcctcgagc cgtgcccagc tgcgctcggc gaggccgagc gacggctcgg
     3601 cgagcacctg gcgcttgacc accacgtcgt cgggtatggc gagaccggcg aacggcgact
     3661 cggcgtcgaa cgggctgagg cgcatcggct tgtcccagct cagcgcgccg ccgagtgcgc
     3721 gcccgccgcc ggccctgagc atcaccggca ccagatcgtc gacccgctcg gcgagccgcg
     3781 ggccggcgaa gcgcaccagc aggccgccgc tctcgatcca cgcggcgagg cgctggcgaa
     3841 tcgcgtccgg aagctggccc acgtccgaca gcatcagcac cgacagcggg cttgcgagca
     3901 ggccgtcgat gtcgccgcgc tgcacctcgg cgaacgggct cagcgcccgg tcgaggtaat
     3961 agagatcgga caggaacggc tggctctgct cgaacgcgcc gccggacacc aggcccaccg
     4021 ggcgtcgccg ccagcgctca tccaccagca ccaccgcgcc ggcgctggac tcgccctcga
     4081 gctcgatgcg cgcgacctcg ttgcgcagct cgccgggcag ctcgagcgcg acctcggcct
     4141 cgaccgcgcc gtcggcgaag gcgaccgcct ggcggccgag cacgcgcccg tcgccgtcgc
     4201 gcgccagcag atacgccgcc gcgcccgcgc gcgccgccgc acgcaccacc cgcaccgtca
     4261 tccgcgccgc cgccgcgtcg ggcgcgagca gcagcttcgc ccgttccgcc ggggcatcgg
     4321 cgtagaccgt gagcgggccg atgtcggcga gccgctcggc gaacctttcg gtcgcgccgg
     4381 gctcggcatc cgccaagccg tcgctcagcc agaccacctc ggcatcgccg agcgcgggca
     4441 gcgacgcggc cagcgcctcc gccgccgccg cccgcgcggc gggccacggc ttgggcgcga
     4501 gcacgcgcac gaagctctgc gcctcgcccg cctcgaccag cccggtcgcg gcgaagccgc
     4561 cgaggccgcc cgcgccctcg gtggtcggcg cggtgcccgc caccgccgcc ggccgatggc
     4621 ggcgctgggc ctcctcgagc agggtccggg cgacgtcgag gcgcttctcc cagcccggcg
     4681 cggccgccca gccgtcgtcg atcaccagca ccaccggccc gtcgaccgcg accgtgcgct
     4741 gggcgttgag cagcggctgg gcgatggcga cgatcaccag cgcggcaagc gcgagccgca
     4801 gcagcagcag ccagagcggg gtatgggtcg gcgtctcctc ggccggcttg agaccgaaca
     4861 ggagccggat tgccgggaac gcgatccgcc gcggcgccgg cgggttgagt cgcagcagcc
     4921 accagatcac cggcagcgcg gcgagcgccg ccaacgccca cggcaccgcg aagctgaggg
     4981 cgccgaggcc ggtcaccgct tgcgcccgag cgcgcccgag agcgcgagat aaagcgccag
     5041 cagcgcggtc tcgggcggcc ggtcggtgcg gtggctgagg aacgtccagc cgctgctccg
     5101 cgccagcgcc gccagcgccg cgcgctgggc ggcgagccga tcgcggtagg ccgcgcgcac
     5161 cgcctcgacc cgcccgatca gcatcgagcc ctcgccctcg gtgccgtcga agcgcacccg
     5221 gccggcgaac ggcaggctct cctcggccgg gtcgagcacc tgcagcaggt ggccggtggc
     5281 gccggtgccg gtcagccagt tcaccgcgcc gtgcaggtcg tcgaccggat cgaggaagtc
     5341 gccgatcatc accacctggg catggcgcgg caggtagtca cggccgggca gcccggcgac
     5401 gccgatcggg gcgtgcatga gggtttgcgc cagccgctcg agcgaggcgg tgccgcgcag
     5461 cggccggcgg ccgtggccaa gcagcgccac ctgctcgccg ccgcgcagca acagcgcggc
     5521 gagcgcgagc agcaacaggg tcgcgcgcgc cgccttggtc tggtcgatgc cgggcgagcg
     5581 ccagtccatc gacggcgagg cgtcgcgcca cagccagacg ctcgccgagg cctcccattc
     5641 ctgctcgcgg acgtagagcg ggaccgactt cgccgactgg cgccagtcga tcaggctggc
     5701 ggcatcgccc ggctggtagc ggcgaaactg ccagaaggtc tcgccgggcc cggcctggcg
     5761 ccggccgtgg acgccttgca gcacggtcgc cgccacccgc cgcgccgcca ccagcagcgc
     5821 cggcagcgcc gccgccgcct gctcggcgcg ctcgcgggcc tgaaccgccg acgccggccc
     5881 ggccattgcc cgccgcgccc tgccgctcag agcagcggtt ggcacatcgc gccgatcacg
     5941 cggtcgatgg tcacgccgtc cgcgcgcgcc gagaaggtga tgtccatgcg gtgcctgagc
     6001 accggcccgg ccagcgccaa cacgtcgtcg atcgacggcg acagccgccc gtcgaggatc
     6061 gcgcgcgcgc ggctggccag catcagcgcc tggctcgccc gcggcccggg cccccaagag
     6121 acgctgtcgc gcaccagctc gatgtcgctg ttccccggcc ggccggcgcg caccagggtc
     6181 aggatcgcgt cgaccacgct gtcgccgacc ggcacctcgc gcacgatgcg ctgggcggcg
     6241 atcagctcgt ccgcggtcat cgcctggcgc ggctccgatt cctcgatgcc ggtggtggcg
     6301 aggatcatgc gccgctcgtc gtcggcgtcc ggatagtcga tgtcgatctg catcaggaac
     6361 cggtcgagct gggcctcggg caatgggtag gtgccttcct gctcgagcgg gttctgggtg
     6421 gcgagcacgt ggaacgggtg cggcagcttg tgcctgatgc cggcgaccga gacctcgttc
     6481 tcctgcatcg cctgcagcag cgccgactgg gtgcgcgggc tcgcgcggtt gatctcgtcg
     6541 gccatcagca gctggcagaa caccggcccg ttgatgaacc tgaagctgcg ccggccggtc
     6601 tctgattcct ccaacacctc ggaaccgatg atgtcggcgg gcatcaggtc gggggtgaac
     6661 tggatgcgct tctcggacag gccgatcacc tggccgagag tctcggtcag ccgggtcttg
     6721 gccaggcccg gcacgccgac caccagcgca tgcccgccgg acaacagcgt gatcaaggtc
     6781 aggtcgacga ccccgtgctg gccgaagatg cgcttgccga cctggcgacg gacctccgcc
     6841 atcttgccgg ccagcgactc gacgcccgcg accaatggcg cgggcggggt gacggacttg
     6901 atggcggcgg gcgcgggatc ggtcatcggg gggcctatgc cttggtgtat cggtcggggc
     6961 tttgcgttaa cgtagtcgca tggccggcac gccaggcgac gatccaaaac agtccgccac
     7021 cgaccccccg cggccggccg gcctcgcgcc ggacggccct caccagcgtg gcgacgtcga
     7081 catccgcatc ggacgcgacg gcaggtggtt ttatcacggt tcgccgattg gccgcaaacc
     7141 gctcgtcaag ctgttcgcct cggtgctcag gcgcgacgag gcgggagatt actggttgca
     7201 gacgccggtc gagaaggcgc gcatccgggt cgacgacgcg ccgttcgtcg ccgtcgcgct
     7261 ggcggtcgaa ggcgaggggc gcgatcaacg gctggtgttc cgctcgagcc tcgacgacgt
     7321 ggtctgcgcc gacgccgacc atccgatccg catcgccgtc gatgcgcgga ccggcgagcc
     7381 cgcgccctac gtcaccgtcc gccccgggct cgaggcgctg atcgcgcgca gcgtcttcta
     7441 ccagctcgtc gacctcgcgg accagtgccc cgagtcccat atcctcggcg tgtggagtgc
     7501 cggaatctgg ttcccgctgg gctcggcggg cgaggcgaca tgacccggga cctggtcatg
     7561 atgaaggtcg gcaagcgcac ggccgggcgc atcattccgg cctcgctcgc ccacggtgag
     7621 ccgagcgcgt cggcggagtc ggcgactatc gaggcggcgg tgctggtgcc gctgatcgac
     7681 cgccccgacg gcatgaccgt gctgctgacc cagcgcagcg agcatctcac cgaccatgcc
     7741 ggccaggtca gctttccagg cgggcgggtc gagcccggcg accgcgggcc ggccgatacc
     7801 gcgctgcgcg aggccgagga ggagatcggt ctcgcgccgc cgcgggtcga ggtggtcggc
     7861 tgcctcgaca tctgcgtcac cggcaccggc ttccgcgtgg tgccggtggt cggcctgatc
     7921 gcggcgccgt tcgacatcgc ccgcgacctc accctcgacg ctttcgaggt cgccgaggcg
     7981 ttcgaggtgc cgctcgcgtt cgtgctcgac gagtcccacc acgagcgccg caccgcgatc
     8041 gcccgcggcg cacggcgcga gttctatgtg cttccctatc aggaccgctt catctggggt
     8101 gccaccgcgc gcatgctggt caatctccat gaggtgctca ggggcaggtc gtgaggacgc
     8161 tcctgacccg gatcctgccg ctgctgctgc cggtcctggt ttatctcggc tggtggtacc
     8221 tcgcccgccg gcgcgcgctg gcgcgcggcg agaccgggcc ggcgctgagc gaggcgccgt
     8281 ggacctggct gttcgtcgcc ggcttcgcgc tgctggtgat gaccttggtg gtgctcggcc
     8341 tgacctgggg cgaggagccg ggcggggtct acgtgccgcc gcgctacgag ggcggcaaga
     8401 tcgttcccgg ccacatcgag cgttgatgcc ctgcacgtga cccccaccgg ccagattgct
     8461 ctgcagccgt ggatgcaggc gccggagacc gcggaggtac tggccgcgct ccaagcgcgc
     8521 ggcaccacgg tgcgcttcgt cggcggctgc gtgcgcgatg cgctgctggg caaggaggtc
     8581 ggcgacatcg acatcgccac ccccgatccg cccgagacgg tgctggcgct gatcgaatcg
     8641 gcgggcctca aggcggtgcc gaccggcctc gcccacggca ccgtcaccgc gatcgcgggc
     8701 ggcgtgccgt tcgaggtcac cacgctgcgc cgcgacgtcg agacctacgg ccgccacgcc
     8761 cgggtcgcgt tcatcgacga ctggaccgac gacgccgcgc gccgcgactt caccatcaac
     8821 gccatgttcc tcgatcccga cggcacgctc tacgacccgt tcggcggccg ggccgacctc
     8881 gccgccggcc atgtgcggtt cgtcggcgag gcgcgccggc gcatcgacga ggacgtgctg
     8941 agattgttgc gcttcttccg cttccatgcg tggtacggcc ggccgcccgc cgacgacgag
     9001 gcgctggccg catgccggga gctggcgccc cggctcgaaa ccttatccgg cgaacgattg
     9061 caggcggaac ttttcaagct gctcgccgcg cccgcgccgt tgccggcgct cgagctcatg
     9121 gccgatgccg gtgtgatgcc gcacctgctg ccaaacgcgc gcgccggctg gcaggcgcgg
     9181 ctcgccgcgc tggtcgggct cgaggacgcg ccggcgaacg gcgacgcggt gcgccggctg
     9241 gcggcgatcg tcgctgcgcc gaccgaggcg ctggcggcgc ggctgagatt gtcgcgcgcc
     9301 gatggcgagc ggctggcggc gctcgcgggc gccgcggagc tgccggcgcc ggcgatggac
     9361 gcgcgcgcgg tgcgggcgat gctctaccgg ctggggcccg agctgttccg cgacctcgcc
     9421 ctgctcgcct ggtcggaagc cggcggcggc gaggacgacg gcttcgcccg cctgctcgcc
     9481 gccgccgccg actggccgtc gcccgagctg ccggtgcgcg ggcgcgacgt gctcgcgcgc
     9541 ggtatcccga agggcacggc ggtcggcgcg ctgctggccg aggtcgagcg ctggtgggtc
     9601 gcgggcgact tccgcgccgg gcgcgcagag gcgctcgcct atctcgacca attgatcgcc
     9661 ggccaaggcc gtggctgagc cggcctgctg ggcgctgacc accggcgagg ccggcgcggt
     9721 cagccaggcg gcaggcctgg ccgaggcggt cggcctgccg tgggtcgcga agcggatcga
     9781 gttgcgccgg ccgtggcgct ggctgccggg gcatctgtgc ccggtcgccc tcgccgggct
     9841 cgggccgggc ggcgaccggc ttgcgccgcc ctggcctgag ctcctgatca gctgcgggcg
     9901 gcgggcggtg gcggcggcga tcgccatccg gcgccgcgcc gccggcggca ccttcgccgt
     9961 ccacctccag cacccgcacg tgccgacgca cctcttcgac atggtggtgc cgccgcgcca
    10021 cgaccgcctt gccggcgcca acgtccatgc cacccgcgtc gccctgcacg ggatcaccgc
    10081 ggccaggctc gcagaggcgg gcgcgcactt ccgcagccgc ttcgcgcacc tgccccggcc
    10141 gctggtcgca gtgctggtgg gcggcgacag tggcagctat cgcctgacgc cggagaccgc
    10201 gcgcgacctc gccgaccggc tcgcggcgct cgcccgggaa gccggggcgg gcctgatcgt
    10261 caccgtctcg cgccgcaccg gggcgggcaa cacggcggtg ctgcgcgcgc gcctcggcgc
    10321 ggccggcgcc gaggtctggg acggcagcgg cgagaacccg tatctcggca tgctcgcgct
    10381 cgccgaccac atcgtggtga ccgccgactc ggtgtcgatg gtctcggagg cgtgcgcgac
    10441 cggcaagccg gtgcacgtct tcccgctccc cggcggcagc gcgcgcttcc gccgcttcca
    10501 cgatgcgctc agcgccgagg gcgtcacgcg accgttctcc gggcggctcg acagctggtc
    10561 gtacccgccg atcgtggagg ccgaagcggt cggcgcaatc gtgcgcgcga agctcgagac
    10621 gcggcgcgcg ccggccgggg ccgccggcgg ctagcg
//
LOCUS       DASWHU010000130         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1536315, whole genome shotgun sequence.
ACCESSION   DASWHU010000130 DASWHU010000000
VERSION     DASWHU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536315"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="VGB88_04585"
     CDS             complement(<1..1606)
                     /locus_tag="VGB88_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9646751.1"
                     /translation="MTAPTTRRRLAAILAADAAGYSRLMQDDEHATVAALNAARAVFR
                     ERIDGHGGRLVDTAGDSVLAVFDSALAAALCALEVQRELAERAREVPESRRMRFRIGL
                     NLGDVIEQADGSIYGDGVNFAARLESLAEPGGLCVSGSVHDMVEGKLDAAFEDIGAQT
                     VKNIARPVRAFRSTLAPVAKAPAWAPVTDRPSIVVLPFANMSADPEQEYFSDGICEDI
                     ITGLSKVSGLFVIARNSSFVYKGRAVNVPEIARELGVRHVLEGSVRKSGRRVRVTAQL
                     IDGKTGGHVWAERYDRELADIFAVQDEVTGAIVTALSVRLTAEERRRTRRHGTDNMDA
                     YDLFLRGREQIWFHSYHAIGTGKSYLEQVLRLDPDFAAAHAMLGFARVIDHVNEWGPE
                     PGSALKDAFVHAQRAIELDEQEPLGHFALALALMWDRRHDEAQARIETALALEPNFMH
                     SHAALGSILMYTGDSKRALASFETAIRLDPHYPDIILHLLGQCHYALGNYQTAKEMFA
                     ERIRRNPSTDASRVMMAAACGMLGEAD"
     gene            1733..2455
                     /locus_tag="VGB88_04590"
     CDS             1733..2455
                     /locus_tag="VGB88_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX9646752.1"
                     /translation="MTEIPAPPPAAVLDALKSISTATVSTQLLKRGLRTHYLVGVRPL
                     NGRHAAFAAPAYTLRYIPAREDRAVPEVWGDREYPQRKAIEAATPGWALVIDARGEMR
                     AGAAGDILIRRLQIRGVAAVVTDGALRDTPVLAAMDFPIFCAGAAAPASVVSLFAADL
                     QVPIGCAGAAVFPGDIVVGDGEGVVVVPRALAEEVARDGAEQERFERFVHSRIEQGRS
                     HFGLYPANEKTLAEYRGWTEDD"
     gene            2492..3478
                     /locus_tag="VGB88_04595"
     CDS             2492..3478
                     /locus_tag="VGB88_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9646753.1"
                     /translation="MNYRKFGRTGIEVSAIVFGGGWVGGILIHKDDETKRMAIRRALD
                     AGINWVDTAPSYGEGKSEEALGWLLQEVDEAPHLSTKVLLDLKRLDDIAGQVESSLAR
                     SFERLRRSSVDLVFLHNPIAPRSDDKRIGINRVLGAGGVIEAFERAREQGLVRFLGIT
                     ALGEAPAVREVIGSGRIDAAQVYYNMLNPSAGEDMPEAWTGHNLSGVIAACKQADCGV
                     MNIRAFAAGVIATDQRTGREVVITDQTRIAEEERKAHALFEALGLDYGTRAQTAIRFS
                     LANPDIDCVAVGMAEYRHLEEAITAAEMGPLPDEALAKVRALYATDFGRVKR"
     gene            complement(3510..4238)
                     /locus_tag="VGB88_04600"
     CDS             complement(3510..4238)
                     /locus_tag="VGB88_04600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9646754.1"
                     /translation="MTDHTGGEIEVRAADGGRFVAYLSVPPARPAPGLLLLPEIYNVN
                     DHIRAVADGFAAQGFVVLAPDVFWRIAPGTWLAYTPEGQAQARALNARLDVDRLVADL
                     GACIAALRARPECTGKVGVAGFCLGGKLAFLCAARHAIDAAVSYYGVKIEDYLDQADR
                     VSCPMVMHFAGNDSHVPPEAVAAITARMAGHDNVAIHVYPGAEHAFNRTGYPPYHEAH
                     AQTALARTLALFRDALAPAAVPGE"
     gene            complement(4268..>4744)
                     /locus_tag="VGB88_04605"
     CDS             complement(4268..>4744)
                     /locus_tag="VGB88_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX9646755.1"
                     /translation="VPTAAFVLGYLLVWGGFSALATGAQWGLVRAALLSPMMAASSVV
                     LGGILLIAAGAWQLTPLKHACLRHCRSPFAFVSRHWRPGAGGAVRMGVLHGAFCLGCC
                     WVLMGLLFYGGVMNPVWIIGLALYVLVEKCAPFGHAVGALSGLALIVWGGMVIAAA"
BASE COUNT          734 a         1643 c         1706 g          661 t
ORIGIN      
        1 cgtccgcctc gccgagcatg ccgcacgcgg ccgccatcat cacccggctg gcatcggtgc
       61 tcggattgcg ccgaatgcgt tcggcgaaca tctccttggc ggtctgatag ttgccgagcg
      121 cgtagtggca ctggccgagc agatgcagga ttatgtccgg gtaatgggga tcgaggcgga
      181 tcgcggtctc gaagctggcg agcgcgcgct tcgagtcgcc ggtgtacatc aggatgctgc
      241 cgagagcggc gtgcgagtgc atgaagttcg gttccagcgc gagcgccgtt tcaatgcgcg
      301 cctgcgcctc gtcgtgacgc cggtcccaca tcaacgcgag cgcaagagcg aaatgaccga
      361 gcggttcctg ctcgtcgagc tctatcgccc gctgggcatg aacgaaggcg tccttcaatg
      421 ccgatccggg ctcgggaccc cattcgttga cgtggtcgat cacgcgcgcg aatccgagca
      481 tcgcgtgcgc cgcggcgaag tcgggatcca accgaagcac ttgctcgaga taggacttgc
      541 cggtaccgat ggcgtggtag gagtggaacc agatctgctc gcggccgcgc aggaacaggt
      601 cgtaggcatc catgttgtcg gtgccgtgac gccttgtccg ccggcgctcc tcggcggtga
      661 gcctcaccga cagcgctgtc acgatcgcgc ccgtgacctc gtcctggacg gcgaagatgt
      721 cggccagctc gcgatcgtag cgctcggccc acacatgccc gccggtcttg ccgtcgatca
      781 gctgggcggt cacgcgcacg cgcctgccgg acttgcgcac gctgccctcg agcacgtggc
      841 ggacgccgag ctcgcgcgcg atctcgggca cgttgaccgc gcgccccttg tagacgaagg
      901 acgagttgcg cgcgatcacg aacaggccgg acaccttgga cagcccggtg atgatgtcct
      961 cgcagatgcc gtcggagaaa tactcctgct cgggatcggc gctcatgttg gcgaacggca
     1021 gcaccacgat cgagggacgg tcggtgaccg gcgcccaagc cggtgccttg gccacgggcg
     1081 caagcgtgct gcggaaggcg cgcacgggcc gggcgatgtt cttgaccgtc tgcgcgccga
     1141 tgtcctcgaa ggcggcgtcg agcttgcctt cgaccatgtc gtggacgctt cccgagacgc
     1201 acagcccgcc cggctcggcg aggctttcca ggcgcgcggc gaagttgacc ccgtcgccgt
     1261 agatgctgcc gtcggcctgc tcgatcacgt cgccgaggtt gagaccgatg cgaaaccgca
     1321 tgcgccgcga ctcgggcacc tcacgcgcgc gctcggcgag ctcgcgctgc acctcgagcg
     1381 cgcagagggc ggccgcgagc gcgctgtcga acaccgccag cacgctgtcg ccggcagtgt
     1441 cgaccaggcg cccgccatgg ccgtcgatgc gctcgcggaa caccgcccgc gccgcattca
     1501 gcgccgccac ggtggcgtgc tcgtcgtctt gcatcaggcg cgaatagccg gccgcgtccg
     1561 cggccaggat cgccgcgagc cgccgccggg tggtcggtgc cgtcatcggt ggcctcgcgt
     1621 ggtaagcgtc gcgcgagtct acggcgggcc gtggaaagat caatcgcgca cgcttccatc
     1681 gccgcccgcc acggggcatg atggccggcg ggacccgggg ggagtgagga cgatgaccga
     1741 gataccggcg ccgccgccgg ctgcggtgct cgacgcgctc aagtcgatca gcacagcgac
     1801 cgtgtcgacc cagctgctca agcgcggcct gcgcacccat tacctggtcg gggtcaggcc
     1861 gctcaacggc aggcatgccg cgttcgccgc gccggcttat acgctccgct acatcccggc
     1921 gcgcgaggac cgcgcggtgc ccgaggtgtg gggcgaccgc gagtacccgc agcgcaaggc
     1981 gatcgaggcg gcgacgccgg gctgggcgct ggtgatcgac gcgcgcggcg agatgcgcgc
     2041 cggcgccgcc ggcgacatcc tgatccggcg cctgcagatc cgcggcgtcg cggcggtggt
     2101 gaccgacggc gcgctgcgcg acacgcccgt gctcgcggcc atggactttc ccatcttctg
     2161 cgccggcgcc gcggcgccgg ccagcgtggt gtcgctgttc gccgccgatt tgcaggtgcc
     2221 gatcggctgc gccggcgcgg cggtctttcc cggcgacatc gtggtcggcg acggcgaggg
     2281 cgtggtggtg gtgccgcgcg cgctcgccga ggaggtggcg cgcgacggcg ccgagcagga
     2341 gcgtttcgag cgtttcgtgc attcgcgcat cgagcagggc cggtcgcatt tcggcctcta
     2401 tccggcgaac gagaagacgc tcgccgagta ccggggctgg accgaggatg actaggccgg
     2461 ccagcgacat cggcgagcgg ggaggagcag cgtgaactat cgcaagttcg gacgtaccgg
     2521 catcgaggtc tccgcgatcg tgttcggcgg cggctgggtc ggcggcatcc tgatccacaa
     2581 ggatgacgag accaagcgca tggcgatcag gcgcgcgctg gatgccggca tcaactgggt
     2641 cgacaccgcg ccgtcctacg gcgagggcaa gtccgaggag gcgctcggct ggctgctgca
     2701 ggaggtcgac gaggcgccgc acctgtcgac caaggtgctg ctggacctca agcgcctcga
     2761 cgacatcgcc ggacaggtcg agagcagcct cgcgcgcagc ttcgagcggc tccggcgcag
     2821 ctcggtcgac ctcgtcttcc tgcacaaccc gatcgcgccc aggagcgacg acaagcgcat
     2881 cggcatcaat cgggtgctgg gtgcgggcgg ggtgatcgag gcgttcgagc gcgcgcgcga
     2941 gcaggggctg gtgcgcttcc tcggcatcac cgcgctcggc gaggcgccgg cggtgcgcga
     3001 ggtaatcggc tcgggccgga tcgacgccgc ccaggtctac tacaacatgc tcaacccgag
     3061 cgccggcgag gacatgcccg aggcgtggac cgggcacaac ctgtccgggg tgatcgccgc
     3121 gtgcaagcag gccgattgcg gggtgatgaa catccgtgcc ttcgccgccg gggtcatcgc
     3181 caccgaccag cgcaccgggc gcgaggtggt gatcaccgac cagacccgga tcgccgagga
     3241 ggagcgcaag gcgcatgccc tgttcgaggc gctcggcctc gactacggca cccgcgccca
     3301 gaccgcgatc cggttctcgc tcgccaatcc cgacatcgac tgcgtcgcgg tcggcatggc
     3361 cgagtaccgc catctcgagg aggcgatcac cgccgccgag atgggcccgc tgcccgatga
     3421 ggcactggcc aaggtcaggg cgctctacgc aaccgatttc gggcgggtca agaggtagtt
     3481 gccgaaccaa gcaacagctc gcgccagggt cactcgcccg gtacggccgc cggggcgagc
     3541 gcgtcccgga acaaggccag cgtgcgcgcc agcgcggtct gcgcgtgcgc ctcgtggtag
     3601 ggcggatagc ccgtgcggtt gaaggcgtgc tcggcgcccg gatagacgtg gatcgcgacg
     3661 ttgtcgtggc cggccatgcg cgccgtgatc gcggcgaccg cctcgggcgg cacgtggctg
     3721 tcgttgccgg cgaagtgcat caccatcggg cacgacacgc ggtcggcctg gtccaggtag
     3781 tcctcgatct tgaccccgta gtagctcacc gcggcgtcga tggcgtggcg cgccgcgcat
     3841 agaaaagcga gcttgccgcc gaggcagaag ccggccacgc cgaccttgcc ggtgcactcg
     3901 gggcgcgcgc gcaaggcggc gatgcaggcg ccgaggtcgg ccaccagccg gtcgacgtcg
     3961 aggcgggcgt tgagcgcgcg cgcctgcgcc tggccctcgg gcgtgtaggc gagccaggtg
     4021 ccgggcgcga tgcgccagaa cacgtccggc gcgagcacca cgaagccttg cgcggcgaag
     4081 ccgtcggcga cggcgcggat gtggtcgttg acgttgtaga tctcgggcag cagcagcagc
     4141 cccggcgccg gccgcgccgg cggcaccgac aagtacgcga cgaagcgtcc gccgtccgcc
     4201 gccctgacct cgatctcgcc gcccgtgtgg tcggtcatgg ccgcctccct catccgcggt
     4261 gagcgcttca agccgccgcg atcaccatac caccccagac gatgagggcg agtccggaca
     4321 gcgcgccgac cgcgtggccg aatggcgcgc acttctcgac cagcacgtag agcgccaggc
     4381 cgatgatcca caccggattc atcaccccgc cgtagaacag cagccccatc agcacccagc
     4441 agcagccgag gcagaacgcg ccgtgcagca cgcccatgcg caccgcgccg cccgcgcccg
     4501 gccgccagtg gcgcgagacg aatgcgaacg gcgaccggca gtggcgcagg caggcgtgct
     4561 tgagcggagt cagctgccag gcgccggcgg cgatcagcag gatgccgccg agcaccacgc
     4621 tcgacgccgc catcatcggc gacagcagcg ccgcgcgcac gagaccccat tgcgcgccgg
     4681 tcgcgagcgc gctgaagccg ccccacacca acagatagcc gagcacgaaa gcggcggtcg
     4741 gcac
//
LOCUS       DASWHU010000131        19717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2644727, whole genome shotgun sequence.
ACCESSION   DASWHU010000131 DASWHU010000000
VERSION     DASWHU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19717
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644727"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /gene="phaZ"
                     /locus_tag="VGB88_04610"
     CDS             complement(<1..1125)
                     /gene="phaZ"
                     /locus_tag="VGB88_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX9646756.1"
                     /translation="MLYHLYELQHSALAPARLLAEHGRALLDHPFNPLGSTPPARFAS
                     AALHMFEHTTRRYGKPAFDIDHTVIDGARVAVAEHVAARKTFGDLKHFRRDVVRDDPP
                     VLLVAPMSGHYATLLRGTVRALLPDHEVYVTDWRDAREVALYEGGFDLDDYIDYLIDF
                     LCVLGPGAHVVAVCQPSVPVLAAVALMAAAGHWAMPASMVLMGGPIDSRANPTEVNTL
                     VEALPLAWFDRNVITRVPWPNRGVMRRVYPGFLQLAGFMSLNLDRHIDAHKDIFRHLV
                     AGDDEGAQAKQAFYEEYRAVMDMTAEFYLQTVELVFKEHALARGRMVSRGRKVDPAAI
                     TDTALMTVEGELDDISKPGQTRAAHLLCPNIPPAMQAHYEQ"
     gene            complement(1255..1767)
                     /locus_tag="VGB88_04615"
     CDS             complement(1255..1767)
                     /locus_tag="VGB88_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9646757.1"
                     /translation="MTQPDNIQLPLSYQIRPGRGGDIAGIQAAARASWHAAYGEIFSA
                     DSIDAFLASAYSRISLRAALANRRSTFLVALHDGRVLGYSQFGDRGAGPEMFRLYVDP
                     RWWGQGIGRRLLSHAEMQFGALGVKRYFLTLHRRNDRAKQFYTSHGFVHHAPRDRDDE
                     LFMIKVLGAP"
     gene            1921..3429
                     /locus_tag="VGB88_04620"
     CDS             1921..3429
                     /locus_tag="VGB88_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX9646758.1"
                     /translation="MPYSSLRDFMARLESLGQLRRVRTPVSPVLEMTEIQTRLLAEGG
                     PAVLFEQPVREDGTAYRMPVLVNLFGTVERVALGMDRTPDRLREVGETLAALRQPEPP
                     GGWREAVGMLPLLKSALAMRPRTVATAPVHEVVLTGADIDLGALPIQTCWPGEPAPLI
                     TWPLVVTRGPGGEREDAFNLGIYRMQVTGRDQTLMRWLRHRGGARHHARWAAEKAEPL
                     PAAAVIGADPATILAAVTPVPETLSEYQFAGLLAGRRLALVDCRTVPLKVPAEAEIVI
                     EGLVALDDQGEEGPYGDHTGYYNAVERFPRFTVTAITRRREPIYLSTFTGRPPDEPSV
                     LAEALNELFVPLIRQQFPEIVDFWLPPEGCSYRIAVVAIRKAYPGHAKRVMMAVWSYL
                     RQFLYTKIVIVVDDDIDARDWTDVMWAIATRMDPARDITVVERTPIDYLDFASPEAGL
                     GGKIGLDATTKMPPETNRPWGRRIRMSDDIIERVTRRWDEYGLPGSGTPIWR"
     gene            3426..4379
                     /locus_tag="VGB88_04625"
     CDS             3426..4379
                     /locus_tag="VGB88_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX9646759.1"
                     /translation="MMSTRQHGGRPAAWLWALAWLSFAAAAWAQDGHVNGQGVLWKVA
                     RDGAADSYLFGTMHTPDARVLALPPAARAALGGAKTVALELLIADAKAASSAGAELAG
                     SMILTDGRTLDAILGPDRFDTVAEALAKMGLPRIAAQRLKPWGAYLMLSFPQASLEPG
                     AAPPTVLDQEIERIARDRGAALVALETVAEQVEVFAGMDEADMVRLLERVIEVGEQEG
                     GLHAYVNGFFDRVTELYLAGDIGGIFEAWTDQLPAGDAALVERLIERLLISRNLTMVE
                     RMTPLLDDGASFVAVGAAHLPGERGVVNLLADLGFTVTPVR"
     gene            4482..5978
                     /locus_tag="VGB88_04630"
     CDS             4482..5978
                     /locus_tag="VGB88_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9646760.1"
                     /translation="MTKAGTDLDLLDDLIAKAKRAGADEADAMLVRATSLEAAWRMGV
                     NEGVERAEARDLGLRVLVGLPSPPCGPKPPAFAKPAPAFAKPAPAGEGRSAGEGRSGD
                     GPKRSPGFAQAGRRQAVVSATEFGAGALAELVERALAMARAVPEDPYCGLPEPARIAR
                     AVADLDLADDHEPSADELLATASEAEDAARAVAGVTNTEGAEASWSRSAVALCASNGF
                     AGSYAGTRFTVVASVIAGSDTAMERDYDYHSARHHGDLEPAGEIGKRAGERAVKRLGA
                     RKQPSGRVPVVFDRRVSGGFLRLLAMSVSGTAVARGTSFLQDKLGETLFAGDIDVIDD
                     PLRPRGLASRPFDGEGVAGARRAVVEHGKLATWLLDSRSARQLGLETTGHAVRGPSSV
                     PAPGPSNLYMAAGAPSPEALIGDIESGFYVTEMMGMSFNPTTGDYSRGAAGFWISHGA
                     IAWPVSEMTVAGNLVEMFAHMTPASDLSFRYGLDAPTLRVDGMTVAGL"
     gene            5975..6784
                     /locus_tag="VGB88_04635"
     CDS             5975..6784
                     /locus_tag="VGB88_04635"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9646761.1"
                     /translation="MSEAARAHAPAPLGTDHALLVAAVRAAGALALDYFRRGFEVREK
                     KPGDPVTDADIAVDRLLAERLCGARPDYGWLSEESPPSPGRERAPRTWIVDPIDGTKG
                     FVEGNSEWVVSAALVEGERPVAAAVFNPATDELFEALAGGGARAGGRPARVTRAASLS
                     GLRLGSSRNEQRRQLWRHLVGEAEVEAIDAIAYKLALVAAGRLDGVIALRPKSEWDIA
                     AGHLLVTEAGGVISDPDGAPLRYNQADPRLPGLIACGPVIYPALRAALARR"
     gene            6889..8664
                     /gene="msbA"
                     /locus_tag="VGB88_04640"
     CDS             6889..8664
                     /gene="msbA"
                     /locus_tag="VGB88_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX9646762.1"
                     /translation="MADAPTVRLIGRLLRGYLAPYRARLAAAVLCMVIAAASQPALAW
                     LMEPVVGEIFVNRDRTMLVLVPLAVLAVMAAGGLANYGQMLLMNWIGLRIVADLQRQV
                     FDHLIRLDLAFFQNTPTGRLIANMTSDANLVRQATSNTLTGLVKDALSVVLLMGLMFY
                     QDWRLALAVLFVFPLSYWPISRLGRRMRKVVVNTQAEIGEFASLLNETFQGARHVKAY
                     GMEDYESGRAGRVIERLFRLYMKSARTRAVMTPIMEALGGIAIALVIFYGGSQVVAGT
                     SEPGAFFAFVTALLLAYRPLKSIANLNTVLQEGLAAATRVFAVLDAKPAIVEAAGARP
                     LCIERGTVRFADVRFAYHAEAPALNGVTLEAEGGTSVALVGPSGAGKSTLLNLIPRFF
                     DVDSGAVLIDGQDVRTVTLASLRGAIALVSQEITLFNDTVRANIAYGAAHAVADQAAI
                     ERVARAAAAHDFITGLARGYDTVVGERGVKLSGGERQRIAIARAMLKDAPILLLDEAT
                     AALDTEAERQVQAALDRLKRGRTTVVIAHRLSTVREADRIYVIDRGRVAEQGRHGELY
                     AAGGLYARLYDLQLGEAPGSADAAE"
     gene            complement(8680..9147)
                     /locus_tag="VGB88_04645"
     CDS             complement(8680..9147)
                     /locus_tag="VGB88_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646763.1"
                     /translation="MRILLVIAALLLPLVSLEARAAAQGDDSVERVFSEIERQVIEEY
                     YRNRGGGDYAEDDWPGGGPKHDKHAAKGGGGLPPGLAKRDRLPPGLEKHLERWGRLPP
                     GLEKRYLPDDLIARLGPRSDQAARQVVGADVLLVDVATGVILDILRDVIAGAE"
     gene            9308..10576
                     /locus_tag="VGB88_04650"
     CDS             9308..10576
                     /locus_tag="VGB88_04650"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX9646764.1"
                     /translation="MLGFYRAATSLGAPLVRLYLHRRARRGREDRARLGERLGQASAP
                     RPDGALVWLHAASVGESASVLPLIERLHQDRSISILVTTVTVTSARLLAERLPGRALH
                     QYLPVDTPAAVRGFLDHWRPDLALWVESELWPNLLAETQARKVPAVMIQGRMSERSFR
                     RWSRWRSLIGPLIAGFRLVVAQTEADAERYRALGARAVTVCSTLKYAALPLAADTVEL
                     NALQRQIGARALWLAASTHAGEEAAAITAHLEARPRLPDLLTFIVPRHPERGPEVAAL
                     ARAQGLTVSLRTAADPITRDTDVYVADTLGELGLFYRLAPLVFVGGSLTDAGGHNPIE
                     PIQLGAATVTGPGQRNFAEVSADLEAASALRTVADGHQLATTVADLLRDPAERAELVR
                     AQKGIIDAKADALEQVMAALKPYIPRHPAA"
     gene            10593..11567
                     /gene="lpxK"
                     /locus_tag="VGB88_04655"
     CDS             10593..11567
                     /gene="lpxK"
                     /locus_tag="VGB88_04655"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9646765.1"
                     /translation="MRAPAFWQTDSAPARALAPLAWAYGVLAGLGARRRRVARAPVPV
                     ICVGNLVAGGAGKTPVALALARRLAARGWRPHLVSRGYRGRAHGPLRVDPARDDAALV
                     GDEALLLAAAAPTWVAGDRAAGAFAAAQAGAGVVVLDDGHQNHSLHKDLSLVVVDGGA
                     GFGNGRLLPAGPLREPVAAGLARAHAAVLVGADRAGVRAALPPALLLLEARLVPAAGA
                     DKVGGRAVVAFAGIGRPEKFFATLESLSCDLLGRHPFADHHRYADHEVLLLADKAVKL
                     GAVLVTTAKDFVRLPAHLRPLVTVLGVEVAWADPERLDRLLDQVAPHG"
     gene            11560..12459
                     /locus_tag="VGB88_04660"
     CDS             11560..12459
                     /locus_tag="VGB88_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl acyltransferase"
                     /protein_id="HEX9646766.1"
                     /translation="MVETRIRPTLRQYAAYALVRVLSALFAALPLDLASGLGGVIGCA
                     VGPLTPMHRRARANLARALPEIGAGARRRILRGMWAHIGRVSAEYPHLGHFRLYDGSG
                     RVEVIGAEHVDALREDGIGGIFVAGHIGNWELASLGATQRGVPLVHVYRAANNPLIER
                     LIQRWRSAVGGVHHPKSPRAVRSLVAALAAGEHLGMLVDQKYNEGIAVPFFGRDAMTA
                     PALAELALKFKVPVVAARVERLKGARFRLTVLPPLDIARTGDRAGDVRAVMTRINALF
                     EDWIRDRPEQWLWMHRRWPENRD"
     gene            complement(12575..13156)
                     /gene="rsmD"
                     /locus_tag="VGB88_04665"
     CDS             complement(12575..13156)
                     /gene="rsmD"
                     /locus_tag="VGB88_04665"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX9646767.1"
                     /translation="MRIVGGALRGRRLKAPPGLGVRPTSDRAREALFNLLAHGAALGG
                     VRLEGAVVLDAFAGTGALGIEALSRGAARAIFVESDRAALAALRANLRALDQGDRALV
                     VARDATHPGAPPAPSSIAFLDPPSGSWLAAPALAALAAAGWLAAGALAVVEHGARERL
                     EAPAGFTAIEQRRYGAAVLTFLRWDRAASPESD"
     gene            complement(13153..13911)
                     /locus_tag="VGB88_04670"
     CDS             complement(13153..13911)
                     /locus_tag="VGB88_04670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9646768.1"
                     /translation="MTKAPAEARMRIARRIARAGLCSRREAERWIADRRVAVNGRVID
                     SPALAVGAGDSIAVDGVEIAAPARARLWRYHKPRGLVTSRRDERGRATVFDSLPEGLP
                     RLISVGRLDLASEGLLLLTNDGALARHLELPATGLVRRYRVRAHGAVDDAALARLGRG
                     MRVDGVDYGPVRIALDRRGAANSWFTVSLREGRNREVRRLFAAIGLEVNRLIRIAYGP
                     FQLGNLAAGAVEEVPAKVLAEQLGRAAADRLAAS"
     gene            complement(13908..14633)
                     /locus_tag="VGB88_04675"
     CDS             complement(13908..14633)
                     /locus_tag="VGB88_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9646769.1"
                     /translation="MTGHGAGPVIGVTLDSEAPGGYSKFPWYALRQNYMATLSAAGAL
                     PVALPHEPDRAEAYLARIDGLVVTGGAFDVDPALFGASSRHASVTTKDRRTDFELAIL
                     RAAVAAEVPVLAICGGAQALNVALGGTLYQHIPDALPGALAHEQANPRDQAGHAVRVV
                     PGSRLHRITGADELMVNSAHHQAVAEPAPGAAINARAPDGVVEGIEISAARFCIGVQW
                     HPEFLISDGDRKLFEAFVAACRT"
     gene            complement(14760..15089)
                     /locus_tag="VGB88_04680"
     CDS             complement(14760..15089)
                     /locus_tag="VGB88_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX9646770.1"
                     /translation="MTISANELQAARAETVRVSGELLRVSLADGRTIVAPLAWFPRLV
                     HATPEERGNWRLIGRGKGIHWADLDEDISVEGLLAGRASGESQESLKAWLHGRSARPP
                     RRTKRRG"
     gene            complement(15144..15305)
                     /locus_tag="VGB88_04685"
     CDS             complement(15144..15305)
                     /locus_tag="VGB88_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX9646771.1"
                     /translation="MPTILRVGPYRFFFYSGDSDEPAHIHVERDDNVAKFWLDPVRLQ
                     GSGGFAPLN"
     gene            15496..16215
                     /locus_tag="VGB88_04690"
     CDS             15496..16215
                     /locus_tag="VGB88_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX9646772.1"
                     /translation="MATAKPSSPVGPFAGFPADFFAFFEELAANNSRAWFADNKQRYQ
                     TEVVDAMVRFIVAMGPLLRKVSPHFVADPRPNGGSMFRIHRDVRFSKDKRPYKEHAAC
                     HFRHAAARDAHAPGFYVHLAPDEIMFGGGIWIPPGDALDDIRAAIDRDRAGWTRVVTD
                     KRLVETFGGVTGEGLKRPPRGYNEGHPHIDDLKRKTFFVIRESGPKAARSPDFVGEVA
                     AAFRDATPLMRFIAGALGQPF"
     gene            16261..16734
                     /locus_tag="VGB88_04695"
     CDS             16261..16734
                     /locus_tag="VGB88_04695"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9646773.1"
                     /translation="MKRREGDAAMARALDEARAAGEAGEVPVGAVVTGPHGELLAAAG
                     NRTERDRDPTAHAELLALRAAAAGLGSPRLEGCDLTVTLEPCPMCAQAASFARIRRLY
                     YGAADPKGGGVDHGPRIFAQATCHHRPEVYGGIAEAEASALLRAFFRSKRARDAG"
     gene            16800..18263
                     /locus_tag="VGB88_04700"
     CDS             16800..18263
                     /locus_tag="VGB88_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX9646774.1"
                     /translation="MARNSTRIPKRKAPGHAVAPAGAAIRKAPFVPARAAPRLGAKRR
                     SSGIAWPVIPAPQHALALAMQYQLEQSQWWPADKLLAHQLRQAQSLIEHAAATVPFYR
                     DRLRPATGLALGALTLERFRDIPLLTRRDVQEAGTAMVSRDLPPGHGTPVPSRTSGSS
                     GRPIEFLNTAVTALMAAAMTLRGHFWHKRELAQKNVSIRPPRLHMPVGRGLWSPPPWN
                     GVSTLIDMRQPISRMFDRMIAEEPVYLQSHPYVILGLIQRSEDTGRRPDKLTEVRTYG
                     ETLAPWLRERCREVWGVPIADNYSAEEMGTIAHQCSGSTNLHVQAENVLVEVLDRDGA
                     PCRPGESGRVVVTALHNFATPFIREELGDIVTAGDPCDCGRGLPVLARILGRERNLVV
                     LPSGEKLFPELYGEFGAVTAVRQYQLIQKTRERIEVRLAVARPLSAEEEATIRGFIRK
                     MFGYAFELPFVYVDEIAREPSGKYQDFRSELPRADGA"
     gene            complement(18285..18914)
                     /locus_tag="VGB88_04705"
     CDS             complement(18285..18914)
                     /locus_tag="VGB88_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX9646775.1"
                     /translation="MTAAGEPTLTFWYELASTYSYPAAFRIERAAAAAGVRVLWRPFL
                     LGPIFRDQGMDTSPFITYPAKGRYMWRDVARLCAAAGVPWRQPSEMPRNSLHAARIAL
                     LGQDQAWGPAFSRAVYRANFADDADIADPEVLAGLLTGLDLPAAELIARAREPATKDR
                     LKAQTAEAAARGIFGSPSYTVSDELFWGGDRLDQALKWARAPGLAAQDP"
     gene            complement(18911..>19717)
                     /locus_tag="VGB88_04710"
     CDS             complement(18911..>19717)
                     /locus_tag="VGB88_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9646776.1"
                     /translation="AIGSQTAGSVIRPASFCGVVGFKPTYDAIALGGVLKLAPALDTL
                     GTFARTVADAGLLAAALGKAGGPLAGIAAGAAQPERVVLCRTHEWDQADEATREVLDS
                     VLARLAGAGVAVDRHDMPRAFDELVEAQNDVMVAEMAASLAEERANHAALLSEGLRAQ
                     LDKGAALGPKRLAERLSYLASCRAQADSLFDDADLLLTAAAPGPAPPAETTGDPLFNR
                     PWTALHLPCLTLPAGTGAGGLPIGAQLVGRNGGDARLVAHGAWIESVLGE"
BASE COUNT         2619 a         7216 c         7214 g         2668 t
ORIGIN      
        1 ctgctcgtag tgggcctgca tggcgggcgg gatgttgggg cacaacaggt gcgcggcgcg
       61 ggtctggccg ggcttggaga tgtcgtcgag ctcgccctcc accgtcatca gcgcggtatc
      121 cgtgatcgcc gccggatcga ccttgcgccc gcgcgaaacc atgcgcccgc gcgccagcgc
      181 gtgctccttg aacacgagct cgacggtctg caggtagaac tccgcggtca tgtccatgac
      241 cgcgcggtac tcctcgtaga acgcctgctt ggcctgggcg ccctcatcgt cgcccgcgac
      301 caggtggcgg aagatgtcct tgtgggcgtc gatgtggcgg tcgaggttga ggctcatgaa
      361 gccggcgagc tggaggaagc cgggatagac ccggcgcatg acgccgcggt tgggccacgg
      421 cacgcgcgtg atcacgttgc ggtcgaacca cgccagcggc agcgcctcga ccagggtgtt
      481 gacctcggtc gggttggcgc ggctgtcgat cggcccgccc atcagcacca tcgaggccgg
      541 catcgcccag tggcccgccg ccgccatcag cgcgaccgcg gcgagcaccg gcaccgacgg
      601 ctggcagacc gcgaccacgt gcgcgccggg gccgagcacg cacaggaagt cgatcaggta
      661 gtcgatgtag tcgtcgaggt cgaagccgcc ctcgtagagg gccacctcgc gcgcgtcgcg
      721 ccagtcggtg acatagacct cgtggtcggg caggagcgcg cgcacggtgc cgcgcagcag
      781 cgtcgcgtag tgccccgaca tcggcgcgac cagcagcacg ggcgggtcgt cgcggacgac
      841 gtcgcgccgg aagtgcttga gatcgccgaa ggtcttgcgc gccgccacgt gctcggcgac
      901 cgccacccgc gcgccgtcga tcacggtgtg gtcgatgtcg aaagcgggct tgccgtagcg
      961 ccgcgtggtg tgctcgaaca tgtgcagcgc cgccgaggcg aaacgcgccg gcggcgtgct
     1021 tccgagcggg ttgaacgggt ggtcgagcag cgcgcgcccg tgctcggcga gcagccgcgc
     1081 cggcgccagc gccgaatgct ggagctcgta gagatggtag agcatgccgg ccccccgttg
     1141 ccgctcagcg cggccgctca gcgcggccgc ggcgcgagta tgagcgcggc gggcgcggca
     1201 ggtccagcca tattgtgcgc tgcaaccaag gcgcgcggtc gcgcgccctt atgtctacgg
     1261 ggcgcctaac accttgatca tgaacaactc gtcgtcacgg tcgcggggcg catggtggac
     1321 gaatccgtgg ctggtataga actgcttggc gcgatcgttt cggcggtgca aggtaaggaa
     1381 gtagcgcttc accccgagcg ccccgaactg catctcggcg tggctcaaca ggcgccggcc
     1441 gatgccctgg ccccaccagc gcgggtcgac gtagaggcgg aacatttccg gccccgcgcc
     1501 gcggtcgccg aactggctgt agccgagcac gcgcccgtcg tgcaacgcca ccaggaaggt
     1561 cgagcggcgg ttggcgagcg ccgccctgag gctgatccgg ctgtaggcgc tggccaggaa
     1621 cgcgtcgatc gagtcggcgg agaagatctc gccgtacgcc gcgtgccagc tggcccgcgc
     1681 cgccgcctgg atgccggcga tgtcgccgcc ccgccccggc cggatctggt agctgagcgg
     1741 cagctggatg ttgtcgggct gggtcaccgg gacgctccca ccgtggtgtc tcgaatatag
     1801 catcgcgccc ggcttgcgcc tgcctgcgcg cgaccgccgc cgcgacaggt gtgacagcgg
     1861 tcacatgcgc ccgtaggatg cgccaagccg acccgtcccg caacccgcac tcagccgtcc
     1921 atgccctaca gttcgctgcg cgacttcatg gcgaggctcg agtccctcgg ccagcttcgc
     1981 cgggtcagga cgccggtctc gccggtgctc gaaatgaccg agatccagac ccggctgctg
     2041 gccgagggcg ggccggcggt gctgttcgag cagccggtgc gcgaggatgg cacggcgtac
     2101 cggatgccgg tgctggtcaa cctgttcggc accgtcgagc gcgtggcgct gggcatggac
     2161 cgcaccccgg accgcctgcg cgaggtcggc gagacgctgg ccgcgctgcg ccagcccgag
     2221 ccgcccggcg gctggcgcga ggcggtgggg atgctgccgc tgctgaagag cgcgctcgcc
     2281 atgcgcccgc gcacggtggc gactgcgccg gtgcacgagg tggtgctgac cggcgccgac
     2341 atcgacctcg gcgcgctgcc gatccagacc tgctggccgg gcgagccggc gccgctgatc
     2401 acttggccgc tggtggtcac ccgcggccca gggggcgagc gcgaggacgc cttcaacctc
     2461 ggcatctacc gcatgcaggt gaccgggcgc gaccagaccc tgatgcgctg gctgcgccac
     2521 cgcggcggcg cccgccacca cgcccgctgg gcggcggaga aggcggagcc gctgcccgcc
     2581 gcggcggtga tcggcgccga cccggcgacc atcctggccg cggtcacgcc ggtgccggag
     2641 accttgtccg agtaccagtt cgccggcctg ctcgccggcc gccgcctggc gctggtcgac
     2701 tgccggacgg tgccgctgaa ggtgccggcc gaggccgaga tcgtgatcga gggcctggtc
     2761 gcgctcgacg accagggcga ggagggcccc tacggcgacc acaccggcta ctacaacgcg
     2821 gtcgagcgct tcccgcgctt caccgtgacc gcgatcaccc gccggcgcga gccgatctac
     2881 ctctcgacct tcaccgggcg cccgcccgac gagccgagcg tgctggctga ggcgctgaac
     2941 gagctgttcg tgccgctcat ccgccagcag ttccccgaga tcgtcgactt ctggctgccg
     3001 cccgagggct gcagctaccg gatcgcggtg gtggcgattc gcaaggccta tccgggccac
     3061 gccaagcggg tgatgatggc ggtctggtcg tacctgcgcc agttcctgta caccaagatc
     3121 gtgatcgtgg tcgacgacga catcgacgcc cgcgactgga ccgacgtgat gtgggccatc
     3181 gccacccgca tggacccggc gcgcgacatc acggtggtcg agcgcacccc gatcgactat
     3241 ctcgatttcg cgagccccga ggccgggctc ggcggcaaga tcggcctcga cgcgacgacc
     3301 aagatgccgc ccgagaccaa ccggccgtgg gggcgcagga ttcgcatgag cgacgacatc
     3361 atcgagcggg tgacccggcg ctgggacgag tacggcctgc ccggcagcgg cactccgatc
     3421 tggcgatgat gagcactcgc caacacggcg gccggcccgc ggcctggctg tgggcgctcg
     3481 cctggctgtc gttcgcagcc gcggcctggg cccaggacgg gcacgtcaat ggccagggcg
     3541 tgctgtggaa ggtggcgcgc gacggcgccg ccgacagcta cctgttcggc accatgcaca
     3601 cccccgatgc gcgcgtgctc gccctgccgc cggcggcgcg cgcggcgctg ggcggggcca
     3661 agacggtcgc gctcgagctg ctgatcgccg acgccaaggc cgcgagcagc gccggcgccg
     3721 agctcgccgg ctcgatgatc ctcaccgacg gccgcaccct cgacgccatc cttgggcccg
     3781 acaggttcga cacggtcgcc gaggcgctgg ccaagatggg cctgccgcgc atcgccgcgc
     3841 agcggctcaa gccgtggggc gcctacctga tgctcagctt cccccaggcc tcgctcgagc
     3901 cgggcgccgc gccgccgacc gtgctcgacc aggagatcga gcgcatcgcc cgcgaccgcg
     3961 gcgccgcgct ggtcgcgctc gagacggtcg cggagcaggt cgaggtgttc gccggcatgg
     4021 acgaggccga catggtgcgc ctgctcgagc gggtgatcga ggtcggcgag caggaaggcg
     4081 ggctgcacgc ctatgtcaac ggcttcttcg accgggtcac cgagctgtac ctcgccggcg
     4141 acatcggcgg catcttcgag gcctggaccg accagcttcc cgccggcgac gcggcgctgg
     4201 tcgagcgcct gatcgagcgc ctgctgatca gccgcaacct gaccatggtc gagcgcatga
     4261 caccgctgct cgacgacggc gccagcttcg tcgccgtcgg cgccgcccac ctgccgggcg
     4321 agcgcggcgt ggtcaacctg ctggccgatc tcggcttcac cgtcacgccg gtgcgctgac
     4381 gcccggcctt cgggccggtc cctggtccgc ccgccgcggc cctgttatcc ggggcgcaaa
     4441 tccccctata taggacccga ccacgctctg gaggcgcgtc gatgaccaag gccggcaccg
     4501 atctcgacct gctcgacgac ctgatcgcca aggccaagcg cgccggcgcc gacgaggccg
     4561 acgcgatgct ggtgcgcgcg acctcgctgg aggcggcgtg gcggatgggg gtcaacgagg
     4621 gcgtcgagcg tgccgaggcg cgcgacctcg gcctgcgggt gctggtcggc ctgccttcgc
     4681 cgccctgcgg cccaaagcct cccgccttcg cgaagcccgc tcccgccttc gcgaagcccg
     4741 ctccggcggg cgaaggaagg tcggcgggcg aaggaaggtc gggcgacggg ccgaagcgaa
     4801 gccccggctt cgcgcaggca ggccggcgcc aggcggtggt ctcggcgacc gagttcggtg
     4861 ccggggcgct cgccgagctg gtcgagcgcg cgctggcgat ggcccgcgcg gtgcccgagg
     4921 acccctattg cggcctgccc gagccggcgc ggatcgcgcg cgcggtcgcg gacctcgact
     4981 tggccgacga ccacgagccg agcgcggacg agctgctcgc cacggcgtcc gaggccgagg
     5041 acgcggcgcg cgcggtcgcg ggcgtcacca acaccgaggg cgccgaggcg agctggtcgc
     5101 ggagcgcggt cgcgctgtgc gcctcgaacg gcttcgccgg cagctatgcc ggcacccggt
     5161 tcaccgtggt cgcgtcggtg atcgccggca gcgacaccgc gatggagcgc gactacgact
     5221 atcacagcgc ccgccaccac ggcgacctag agcccgcggg cgagatcggc aagcgcgccg
     5281 gcgagcgggc ggtcaagcgg ctgggcgcgc gcaagcagcc gagcgggcgg gtgccggtgg
     5341 tgttcgaccg gcgcgtctcg ggcggcttcc tgcgcctgct ggcgatgtcg gtctcgggca
     5401 ccgcggtcgc gcgcggcacg agttttcttc aggacaagct cggcgagacg ctgttcgccg
     5461 gcgacatcga cgtcatcgac gacccgctgc gcccgcgcgg cctcgcctcg cgcccgttcg
     5521 acggcgaggg cgtcgccggc gcgcgccgcg ccgtggtcga gcacggcaag ctcgccacct
     5581 ggctgctcga cagccgctcg gcgcgccagc tcgggctcga gaccaccggc cacgccgtgc
     5641 gcgggccgtc gtcggtgccg gcgccggggc cgagcaacct gtacatggcc gccggcgcgc
     5701 cctcgccgga ggcgctgatc ggcgacatcg agtcgggctt ctacgtcacc gagatgatgg
     5761 gcatgagctt caacccgacc accggcgact acagccgcgg cgccgccggg ttttggatct
     5821 cgcacggcgc catcgcctgg ccggtgagcg agatgacggt cgccggcaac ctggtcgaga
     5881 tgttcgcgca catgaccccg gccagcgact tgagcttccg ctacgggctg gacgcgccga
     5941 ccttgcgcgt cgacggcatg acggtggccg ggttgtgagc gaggccgcgc gggcacacgc
     6001 gccggcgccg ctcggcaccg accacgcgct gctggtcgcg gcggtacgcg ccgcgggcgc
     6061 gctggcgctc gactatttcc ggcgcggctt cgaggtgcgc gagaagaagc cgggcgaccc
     6121 ggtcaccgac gccgacatcg ccgtcgaccg gctgctcgcc gagcgcctgt gcggcgcccg
     6181 cccggactat ggctggctgt cggaggagag cccgccctcg cccgggcgcg agcgcgcgcc
     6241 gcggacctgg atcgtcgatc cgatcgacgg caccaagggc ttcgtcgagg gcaacagcga
     6301 gtgggtggtc tcggcggcgc tggtcgaggg cgagcgcccg gtggcggcgg cggtgttcaa
     6361 cccggcgacc gatgagctgt tcgaggcgct cgccggcggc ggcgcgcgcg cgggcgggcg
     6421 cccggcgcgg gtgacgcgcg ccgcctcgct cagcgggctc aggctcggct cgagccgcaa
     6481 cgagcagcgc cgccagctgt ggcggcacct ggtcggcgag gccgaggtcg aggcgatcga
     6541 cgccatcgcc tacaagctcg ccctggtcgc cgccgggcgg ctcgacggcg tgatcgcgct
     6601 caggcccaag tcggagtggg acatcgccgc cggccatctg ctggtcaccg aggcgggcgg
     6661 cgtgatcagc gaccccgacg gcgcgccgct ccgctacaac caggccgatc cgcgcctgcc
     6721 cggcctgatc gcctgcgggc cggtgatcta cccggcgctg cgtgccgcgc tcgcccggcg
     6781 ctgaggcggc ggctttggcg tcggccggcc ccatgctagg ctcggccatc gcgatgcaca
     6841 gatcgccaga cattgaccgc cggcccatcc gtccctagcg ccagcgccat ggccgatgcg
     6901 ccgaccgtgc gcctgatcgg gcggctgctg cgcggctacc tggcgcccta tcgcgcgcgg
     6961 cttgccgcgg cggtcctgtg catggtcatc gccgccgcct cgcagcccgc gctcgcctgg
     7021 ctgatggagc cggtggtggg cgagatcttc gtcaaccgcg accgcaccat gctggtgctg
     7081 gtgccgctgg cggtgctcgc ggtgatggcc gcgggcgggc tcgccaatta cggccagatg
     7141 ctgctgatga actggatcgg cctgcgcatc gtcgccgacc tgcagcgcca ggtgttcgac
     7201 cacctgatcc gcctcgacct ggcgttcttc cagaacaccc cgaccgggcg gctgattgcc
     7261 aacatgacca gcgacgccaa cctggtgcgc caggcgacct cgaacacgct caccggcctg
     7321 gtcaaggacg cgctcagcgt ggtgctgctg atggggctga tgttctatca ggactggcgg
     7381 ctggcgctgg cggtgctgtt cgtgttcccg ctcagctact ggccgatcag ccggctcggg
     7441 cggcggatgc gcaaggtggt ggtcaacacc caggccgaga tcggcgagtt cgcctcgctg
     7501 ctcaacgaga ccttccaggg cgcgcgccac gtcaaggcct acggcatgga ggactacgag
     7561 agcgggcgcg ccgggcgggt gatcgagcgc ctgttccgcc tgtacatgaa gtcggcgcgc
     7621 acgcgcgcgg tgatgacgcc gatcatggag gcgttgggcg gcatcgccat cgcgctggtg
     7681 atcttctacg gcggcagcca ggtggtcgcg gggaccagcg agccgggcgc gttcttcgcc
     7741 ttcgtcaccg cgctgctgct cgcctaccgg ccgctcaaga gcatcgccaa cctcaacacc
     7801 gtgctccagg aggggctcgc cgccgcgacc cgcgtgttcg cggtgctcga cgccaagccc
     7861 gcgatcgtcg aggcggcggg cgcccggccg ctgtgcatcg agcgcggcac ggtccgcttc
     7921 gccgacgtcc gcttcgccta tcacgccgag gcgccggccc tgaacggggt cacgctcgag
     7981 gccgagggcg gcacctcggt cgccctggtc gggccgtcgg gcgccggcaa gtcgacgctc
     8041 ctcaacctga tcccgcgctt cttcgacgtc gattcgggcg cggtgctgat cgacggccag
     8101 gacgtgcgca cggtgacgct cgcgtccttg cgcggcgcga tcgccctggt cagccaggag
     8161 atcaccctgt tcaacgacac ggtgcgcgcc aacatcgcct acggcgccgc gcacgccgtc
     8221 gccgaccagg cggcgatcga gcgcgtcgcc cgcgccgccg ccgcgcacga cttcatcacc
     8281 ggcctcgcgc gcggctacga caccgtggtc ggcgagcgcg gggtcaagct gtcgggcggc
     8341 gagcgccagc gcatcgcgat cgcgcgcgcg atgctcaagg acgcgccgat cctgctcttg
     8401 gacgaggcca ccgccgcgct cgataccgaa gccgagcgcc aggtgcaggc ggcgctcgac
     8461 cggctcaagc gcgggcgcac gacggtggtg atcgcgcacc ggctgtcgac cgtgcgcgag
     8521 gccgaccgca tctacgtgat cgaccgcggg cgggtggccg agcagggccg ccacggcgag
     8581 ctctatgccg cgggcggcct ttatgcgcgg ctctacgacc tgcagctcgg cgaggcgccg
     8641 ggctccgccg acgccgccga gtgagcgagc gagacggggc tactcggcgc cggcgatcac
     8701 gtcgcgcagg atgtctagga tcacgccggt ggcgacgtcg accaggagca cgtcggcgcc
     8761 gaccacctgg cgcgcggcct ggtcgctgcg cgggccgaga cgcgcgatca ggtcgtcggg
     8821 cagataacgc ttctcgagcc cgggcggcag ccggccccag cgctcgaggt gcttctcgag
     8881 gccgggcggc aagcggtcgc gcttggccag gcccggcggc aggccgccgc cgccctttgc
     8941 ggcatgcttg tcgtgcttcg gcccgccgcc cggccagtcg tcctcggcgt agtcgccccc
     9001 gccgcgattc cggtaatatt cctcgatgac ctgccgctcg atctccgaga acacccgctc
     9061 gaccgagtcg tcgccctggg cggccgcccg tgcctcgagc gataccagcg gcagcagcag
     9121 ggccgcgatg actaaaagaa tgcgcatctc gatcctcgtt gaatatgatt tcgcccactt
     9181 cgttaccgca ggccggctga acggcggctg aacggcgatc ggggcggggg ccacgcgcgg
     9241 gaccgattgt caacatggat cggccgcggc gaatcacaag ccgggccgag cgcggatggg
     9301 ggcgggcatg ctgggtttct acagggcggc gaccagcctc ggcgcgccgc tggttcgcct
     9361 ttatctgcac cggcgcgcgc gccgcggccg ggaggaccgg gcgcggctgg gcgagcggct
     9421 cgggcaggcc tcggcgccgc gccccgacgg cgccctggtg tggctgcacg cggccagcgt
     9481 cggcgaatcg gcgtcggtgc tgccgttgat cgagcgcctg caccaggacc gctcgatcag
     9541 catccttgtg accacggtca cggttacctc ggcgcgcctg ctcgccgagc gcttgcccgg
     9601 gcgggcgctc caccagtacc tgccggtcga cacgccggcg gcggtgcgcg gcttcctcga
     9661 ccactggcgc cccgacctcg cgctgtgggt cgagtcggag ctgtggccca acctgctcgc
     9721 cgagacccag gcgcgcaagg tgccggcggt gatgatccag ggccggatgt ccgagcgctc
     9781 gttccggcgc tggagccggt ggcggtcgct gatcgggccg ctgatcgcgg gcttccggct
     9841 ggtggtcgcg cagaccgagg ccgacgccga gcgctaccgg gcgctgggcg cgcgcgcggt
     9901 gacggtgtgc agcacgctca aatacgcggc cttgccgctc gccgccgaca ccgtcgagct
     9961 gaacgcgttg cagcgccaga tcggggcgcg ggcgctgtgg ctcgcggcga gcacccatgc
    10021 cggcgaggag gcggcggcga tcaccgccca tctcgaagcg aggcccaggc tgccggacct
    10081 gctcactttc atcgtcccgc gccatcccga gcgcgggccc gaggtcgcgg cgctcgcccg
    10141 cgcccagggg ctcaccgtga gcctgcgcac cgccgccgac ccgatcaccc gcgacaccga
    10201 cgtctatgtc gccgataccc tgggcgagct cggcctgttc taccggctgg cgccgctggt
    10261 gttcgtcggc ggctcgctca ccgacgccgg cggccacaac ccgatcgagc cgatccagct
    10321 cggcgccgcg accgtgaccg ggcccgggca gcgcaacttc gccgaggtct cggccgacct
    10381 cgaggcggcc tcggcgctgc gcaccgtcgc cgacggccat cagctcgcca ccaccgtcgc
    10441 cgacctgctg cgcgacccgg cggagcgggc cgagctggtg cgcgcccaga agggcatcat
    10501 cgacgccaag gcggacgcgc tcgagcaggt gatggcggcg ctcaagccct acatcccccg
    10561 gcacccggcg gcgtgatacg cccgcgacag cggtgagggc accggccttc tggcagaccg
    10621 acagtgcgcc cgcgcgcgcg ctcgcgccgc tcgcctgggc ctacggcgtg ctcgccgggc
    10681 tgggtgcccg gcgccggcgg gtggcgcgcg cgccggtgcc ggtgatctgc gtcggcaacc
    10741 tggtcgcggg cggcgccggc aagacgccgg tggcgctggc gctggcccgg cggctcgccg
    10801 cgcgcggctg gcggccgcac ctggtcagcc gcggctatcg cgggcgcgcc cacggaccgc
    10861 tcagggtcga ccccgcccgc gacgacgcgg cgctggtcgg cgacgaggcg ctgctgctcg
    10921 ccgcggccgc gccgacctgg gtcgccggcg accgcgccgc cggcgctttt gccgcggccc
    10981 aggcgggcgc gggcgtggtg gtgctcgacg acggccatca gaaccacagc ctgcacaagg
    11041 acctgtcgct ggtggtggtc gacggcggcg cgggtttcgg caacggccgg ctgctgccgg
    11101 cgggcccgct gcgcgagccg gtggcggcgg gcctcgcccg cgcccatgcg gcggtgctgg
    11161 tcggcgccga ccgcgccggc gtgcgcgccg ccctgccgcc cgcgctgttg ctgctggagg
    11221 cgcggctggt cccggcggcc ggcgccgaca aggttggcgg gcgggcggtg gtcgcgttcg
    11281 ccggcatcgg ccggcccgag aagttcttcg ccacgctcga gtcgttgtca tgcgatctgt
    11341 tgggccgcca cccgttcgcc gaccatcacc gctatgccga ccacgaggtc ctgctgcttg
    11401 ccgacaaggc ggtgaagctc ggcgcggtgc tggtgaccac cgccaaggac ttcgtccgcc
    11461 tgccggcgca tctcaggccg ctggtcacgg tgctcggggt cgaggtggcg tgggccgacc
    11521 cggagcggct cgaccgcctg ctcgaccagg tggcgccgca tggttgagac gcgcatccgc
    11581 ccgaccttgc gccagtacgc cgcgtacgcg ctggtgcgcg tcttgtcggc gctgttcgcg
    11641 gcgctgccgc tcgacctcgc ctcgggcctc ggcggcgtga tcggatgcgc ggtcgggccg
    11701 ctgacgccga tgcatcggcg ggcgcgcgcc aacctcgccc gggcgctgcc cgagatcggc
    11761 gccggcgcgc ggcggcgaat cctgcgtgga atgtgggcgc atatcgggcg ggtgtccgcc
    11821 gagtacccgc atctcggcca cttccgcctc tatgacggca gcggccgggt cgaggtgatc
    11881 ggcgccgagc atgtcgatgc gctgcgcgag gacggcatcg gcggcatctt cgtcgccggg
    11941 cacatcggca actgggagct cgcctcgctc ggcgccaccc agcgcggggt gccgctggtc
    12001 cacgtctacc gcgccgccaa caacccgctc atcgagcgcc tgatccagcg ctggcggagc
    12061 gccgtcggcg gcgtccatca cccgaaaagc ccgcgcgccg tgcgcagcct ggtcgcggcg
    12121 ctggcggcgg gcgagcatct gggcatgctg gtcgaccaga agtacaacga gggcatcgcc
    12181 gtgcccttct tcggccgcga cgcgatgacc gcgccggcgc tcgccgagct cgcgctcaag
    12241 ttcaaggtgc cggtggtggc ggcgcgggtc gagcggctca agggcgcgcg cttccggctc
    12301 accgtgctgc cgccgctcga catcgcgcgc accggcgacc gcgccggcga cgtgcgcgcg
    12361 gtcatgaccc gcatcaacgc gctgttcgag gactggatcc gcgaccgccc cgagcagtgg
    12421 ctgtggatgc accgccgctg gcccgaaaac cgggactgag gaaggtttaa ccacagaaga
    12481 cacagagagc acagagatag aaagtgttgc tagtcagcta actgttttct ttacagttat
    12541 cgcctttcct ctgtgacctc tgtgtcctct gtggtcaatc cgattccggc gacgcggccc
    12601 gatcccaacg caggaaggta agcacggcgg cgccgtagcg gcgctgctcg atcgcggtga
    12661 agccggccgg cgcctccagg cgctcgcgcg cgccgtgctc gaccaccgcc agcgcgcccg
    12721 cggcgagcca gccagcggcg gcgagcgccg ccagcgccgg cgcggcgagc cacgagccag
    12781 agggcgggtc gaggaaggcg atcgagcttg gcgccggcgg cgcgcccgga tgggtcgcat
    12841 cgcgcgccac caccagcgcc cggtccccct ggtcgagggc gcgcaggttg gcgcgcaacg
    12901 ccgccagcgc ggcgcggtcg ctctcgacga agatcgcgcg cgcggccccc cgcgacagcg
    12961 cctcgatccc gagcgcgccg gtgccggcga aggcgtcgag caccaccgcg ccctcgagcc
    13021 gcacaccgcc gagcgcggcg ccgtgggcga gcaggttgaa cagcgcctcg cgcgcgcggt
    13081 ccgaggtcgg gcgcacgccg agccccggcg gcgctttcag cctgcgcccg cgcaaggcac
    13141 cgccgacgat cctcacgagg ccgccaggcg gtccgcggcc gcgcgcccga gctgctcggc
    13201 gagcaccttg gccggcacct cctcgaccgc gcccgccgcc aggttgccga gctggaacgg
    13261 gccgtaggcg atgcggatca gccggttgac ctcgagcccg atggcggcga acaggcgccg
    13321 gacctcgcgg ttgcggccct cgcgcaagga caccgtgaac cagctgttgg ccgcgccgcg
    13381 gcggtcgagc gcgatgcgca ccggcccgta gtcgaccccg tccacgcgca tgccgcggcc
    13441 gagccgcgcg agcgcggcgt cgtcgaccgc gccgtgggcg cgcacccggt agcggcgcac
    13501 caggccggtc gccggcagct cgagatggcg ggcgagcgcg ccgtcgttgg tcagcagcaa
    13561 caggccctcc gaggcgaggt cgagccggcc gaccgagatc aggcgcggca ggccctcggg
    13621 cagcgagtcg aacaccgtcg cccggccacg ctcgtcgcgg cggctggtga ccaggccgcg
    13681 cggcttgtgg tagcgccaca ggcgcgcgcg cgcgggcgcg gcgatctcga cgccatcgac
    13741 cgcgatgctg tcgcccgcgc cgaccgcgag cgccgggctg tcgatcaccc ggccgttgac
    13801 ggcaacccgc cggtcggcga tccagcgctc ggcctcgcgc cgcgagcaca gaccggcgcg
    13861 cgcgatccgg cgggcgatgc gcatgcgcgc ctcggccggc gccttggtca cgtccggcag
    13921 gcggcgacga acgcctcgaa cagcttgcgg tcgccgtcgc tgatcaggaa ctcgggatgc
    13981 cactggacgc cgatgcagaa gcgcgcggct gagatctcga tgccctcgac caccccgtcc
    14041 ggggcgcggg cgttgatcgc ggcgccgggt gccggctcgg cgaccgcctg gtggtgggcg
    14101 ctgttgacca tcagctcgtc ggcgccggtg atccggtgca gccggctgcc cggcaccacg
    14161 cgcacggcgt ggcccgcttg atcgcgcggg ttggcctgct cgtgcgcgag agcgccgggg
    14221 agtgcgtccg ggatgtgctg gtagagggtg ccgccgagcg ccacgttcag ggcctgcgcg
    14281 ccgccgcaga tggcgagcac cggcacctcg gccgcgaccg cggcgcgcag gatggcgagc
    14341 tcgaagtcgg tgcggcggtc cttggtggtg acgctcgcat ggcggctgga tgcgccgaac
    14401 agcgccgggt cgacgtcgaa cgcgccgccg gtgaccacca ggccgtcgat gcgcgcgaga
    14461 taggcctcgg cgcggtcggg ctcgtggggc agcgccaccg gcagcgcgcc ggcggcactg
    14521 agcgtcgcca tgtagttctg gcgcagcgcg taccagggga acttggagta tccgccgggc
    14581 gcctcgctgt cgagggtcac cccgatcacc ggcccggcgc cgtgtccggt catggcggtg
    14641 aagataggcg cggggcgcgg ggcgcgcaac gcgggcgtcg tttgagtagc gtcccagctt
    14701 gtgccgcgca gaatattccg gtggtcacga cgccgacctc gcggcgaccg tccgcgccgt
    14761 caaccccgcc gcttggtccg ccggggggga cgcgcactgc ggccgtgaag ccacgccttc
    14821 agggactcct gactctcgcc cgaggctcgc cccgcgagca gcccctcgac gctgatgtcc
    14881 tcgtcgaggt cggcccagtg aatccctttg cctcgcccga tcaggcgcca attgccgcgt
    14941 tcctcgggag tcgcgtggac gagcctcgga aaccatgcca gcggcgcgac gatcgtccga
    15001 ccgtcggcca ggctcacgcg cagcaattcg ccgctgaccc tgacggtctc cgccctcgcg
    15061 gcctgcaatt cattcgccga aatagtcatg ccatgcctta atcaacgcct ctcggcgatc
    15121 ctgcacatgc tgctgaatcc gcctcagttc aggggcgcaa accctccgct gccctgcaag
    15181 cgcaccggat cgagccagaa cttggcgacg ttgtcatctc gctccacgtg aatatgcgct
    15241 ggttcgtcgc tgtcacctga gtagaagaag aaccggtacg gacccaccct caggatcgtc
    15301 ggcatccgcc cctccctatg ggccgttcat cggagcacga ttacgaggcc cggccccggg
    15361 tgcggtcatg tccgggaaga taggcgcagg ccccggcgcg cgcaacgcgg acccggaccg
    15421 gctgcgcgat gcgctggcgg gcgggcgccg ccggctggca tactggggcg gtccatctgc
    15481 gtgccggcat ccgtcatggc gacagccaag ccttcgtccc cggtcgggcc attcgcgggc
    15541 tttccggccg acttcttcgc cttcttcgag gagctggcgg cgaacaacag ccgcgcctgg
    15601 ttcgccgaca acaagcagcg ctaccagacc gaggtggtcg atgcgatggt gcgcttcatc
    15661 gtcgccatgg gcccgctctt gcgcaaggtc tcgccccatt tcgtcgccga tcccaggccc
    15721 aacggcggct cgatgttccg catccaccgc gacgtgcgct tctcgaagga caagcggccg
    15781 tacaaggagc atgccgcctg ccatttccgg cacgcagccg cgcgcgacgc ccatgcgccc
    15841 ggcttctacg tccatctcgc ccccgacgag atcatgttcg gcggcggcat ctggataccg
    15901 cccggcgacg ccctcgacga catccgtgca gcaatcgacc gcgaccgcgc cggctggacg
    15961 cgcgtggtca ccgacaagcg cctagttgag accttcggcg gcgtcaccgg cgagggtctc
    16021 aagcggccgc cgcgcggcta caacgagggt catccccata tcgacgacct caagcgcaag
    16081 accttcttcg tgatcaggga gagcgggccc aaggccgcgc gctcgccgga cttcgttggc
    16141 gaggtggccg cggcgttccg cgacgccacg ccgctgatgc gattcatcgc cggggcgctc
    16201 ggtcagccgt tctgagcgcc ccgcttgacc gggcgcacgc ccgccgcgca gggtgcggcg
    16261 atgaagaggc gcgagggcga cgctgccatg gcgcgcgcgc tcgacgaggc gcgggcggcg
    16321 ggcgaggccg gcgaggtgcc ggtcggcgcg gtggtcaccg gcccccacgg cgagctgctg
    16381 gcggcggccg gcaaccgcac cgagcgcgac cgcgacccga ccgcccatgc cgagctgctc
    16441 gcgctgcgcg ccgccgccgc cgggctcggc tcgccgaggc tcgagggctg cgacctcacc
    16501 gtcaccctgg agccgtgccc gatgtgcgcc caggcggcct cgttcgcccg catccgcagg
    16561 ctctactacg gcgccgccga ccccaagggc ggcggcgtcg accacggccc gcgcatcttc
    16621 gcgcaggcga cctgccacca ccgccccgag gtctatggcg gcatcgccga ggccgaggcg
    16681 tcggcgttgt tgcgcgcctt cttccgctcg aaacgcgcgc gcgacgccgg atagcggcgg
    16741 caggaggccc tatatcctaa cggttgccgg cggcccgacg ccggtgaggg cggctcgcga
    16801 tggcacgaaa cagcacccgg atcccgaagc gcaaggcccc cggacatgcc gtcgcgccgg
    16861 cgggcgcggc gatccgcaag gcgccgttcg tgccggctcg cgccgcgccc cggctgggcg
    16921 ccaagcgccg gtcgagcggc atcgcctggc cggtcattcc ggcaccgcaa cacgcgctgg
    16981 cgctggcgat gcagtatcag ctcgagcagt cccaatggtg gccggcggac aagctgctcg
    17041 cccaccagct gcgccaggcg cagagcctga tcgagcacgc cgcggcgacg gtgccgttct
    17101 atcgcgaccg gctccggccg gcgaccggcc tggcgttggg cgcgctgacg ctcgaacgct
    17161 tccgcgacat cccgctgctt acccgccggg acgtccagga ggccggcacg gcgatggtga
    17221 gccgggacct gccgccgggc cacggcacgc cggtcccgtc caggacctcg ggctcgtccg
    17281 gccggccgat cgagttcctc aacaccgccg tcaccgcgct catggcggcg gccatgaccc
    17341 tgcgcggcca cttctggcac aagcgcgagc tggcgcagaa gaacgtgtcg atcaggccgc
    17401 cgcgcttgca catgccggtc gggcgcgggc tgtggtcgcc gccgccgtgg aacggggtca
    17461 gcacgttgat cgacatgcgc cagccgatca gccgcatgtt cgaccggatg atcgccgagg
    17521 agccggtcta cctgcagtcc cacccctatg tgatcctggg cctgatccag cgctccgagg
    17581 acaccgggcg ccggcccgac aagctcacgg aggtgcggac ctacggcgag acgctggcgc
    17641 cgtggctgcg cgagaggtgc cgcgaggtct ggggcgtgcc gatcgccgac aactacagcg
    17701 ccgaggaaat gggcacgatc gcgcaccaat gctccggctc gaccaacctg cacgtccagg
    17761 cggagaacgt gctggtcgag gtcctcgacc gcgacggcgc gccgtgccgg ccgggcgaga
    17821 gcgggcgcgt ggtggtgacc gcgctgcaca acttcgccac cccgttcatc cgcgaggagc
    17881 tcggcgacat cgtcaccgcc ggggaccctt gcgactgcgg gcgcgggctg ccggtgctcg
    17941 cgcgcatcct cggccgcgag cgcaacctgg tcgtccttcc ctccggcgag aagctgtttc
    18001 ccgagctcta cggcgaattc ggcgcggtga ccgccgtccg ccagtaccag ctgatccaga
    18061 agacgcgcga gcggatcgag gtgaggctcg ccgtcgcccg cccgctcagc gccgaggagg
    18121 aggcaaccat ccgcggcttc atccgcaaga tgttcggcta tgccttcgag ctgccgttcg
    18181 tctatgtcga cgagatcgcg cgcgagccga gcggcaagta tcaggacttc cgctcggagt
    18241 tgccgcgagc ggacggcgcc tgaccgtcga cgcggcctgc accgtcacgg gtcctgtgcc
    18301 gccagccccg gcgcgcgggc ccatttgagg gcctggtcga ggcggtcgcc gccccagaac
    18361 agctcgtcgc tgacggtgta gctcggcgag ccgaagatgc cgcgcgccgc ggcctcggcg
    18421 gtctgcgctt tcagccggtc cttggtcgcg ggctcgcgcg cgcgcgcgat cagctccgcc
    18481 gccggcaggt cgaggcctgt caggagcccg gcgagcacct cggggtcggc gatgtcggca
    18541 tcgtcggcga agttcgcccg gtagaccgca cggctgaacg ccggccccca ggcctggtcc
    18601 tggccgagca gcgcgatgcg cgcggcgtgg agcgagttgc gcggcatctc gctcggctgc
    18661 cgccagggca cgcccgccgc cgcgcacagg cgcgcgacgt cgcgccacat gtagcgcccc
    18721 ttggccggat aggtgatgaa cggcgaggtg tccatgccct ggtcgcggaa gatcgggccg
    18781 agcaggaacg gccgccacag cacccggacg ccggccgcgg ccgccgcgcg ctcgatgcgg
    18841 aacgcggccg gatagctgta ggtgctggcg agctcgtacc agaaagtgag ggtgggctcg
    18901 cccgctgcag tcattcgccg agcaccgatt cgatccacgc gccgtgggcg acgagccggg
    18961 cgtcgccgcc gttgcgcccg accagctggg cgccgatcgg caagccgccc gcgccggtgc
    19021 ccgcgggcaa ggtcaggcac ggcagatgca gcgccgtcca cggccggttg aacagcgggt
    19081 cgccggtggt ctcggccggc ggcgccgggc cgggcgcggc cgcggtgagc agcaggtcgg
    19141 cgtcgtcgaa cagcgagtcc gcctgcgccc ggcagctggc gagatagctg agccgctcgg
    19201 cgagccgctt gggcccgagc gcggcgccct tgtcgagttg cgcgcgcagg ccctcgctca
    19261 acagcgccgc gtggttggcg cgctcctcgg ctagcgaggc cgccatctct gcgaccatga
    19321 cgtcgttctg cgcctcgacc agctcgtcga acgcgcgcgg catgtcgtgg cggtcgaccg
    19381 cgacaccggc gccggcgagg cgggcgagca ccgaatcgag cacctcgcga gtcgcctcat
    19441 ccgcctggtc ccattcatgg gtgcggcaca gcaccacccg ctcgggctgc gccgcgccgg
    19501 cggcgatccc ggcgagcggc ccgcccgcct tgccgagcgc ggcggcgagc aggcccgcgt
    19561 ccgcgaccgt gcgcgcgaag gtgccgaggg tgtcgagcgc cggcgcgagc ttcagaacac
    19621 cgccaagcgc gatcgcgtcg tacgtcggct tgaagccgac cacgccgcag aacgacgccg
    19681 ggcggatcac cgagcccgcg gtctggctgc cgatcgc
//
LOCUS       DASWHU010000132         8433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3020829, whole genome shotgun sequence.
ACCESSION   DASWHU010000132 DASWHU010000000
VERSION     DASWHU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020829"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1380
                     /locus_tag="VGB88_04715"
     CDS             187..1380
                     /locus_tag="VGB88_04715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9646777.1"
                     /translation="MERNPPQLSCADPALASGAASRPAAELRRLNVFEPDFGIEEEQA
                     LVAVLRSGWVAMGPKVEAFERRFADLVGTQHAVAVSSCTAALHLTQVALGIGRGDEVI
                     VPSLTFSATANSALVTGATPVFADIASADDWTLDPADAARRITPRTKAVIAVHYGGHP
                     ADMAALGAVCRDAGVTLIEDACHGLGGSLDGRAMGALGAAGCFSFYSNKVITTGEGGM
                     VTTDDADLALHIAKLRSHGMTATAIDRVRGAFDYEVTEAGFNYRLDDLRAAVGLAQLD
                     RLAVKLARRRDLVRLYCARLASIPGLSVPNFGGRGEPANYLMTVLLDGGDREAVRRRL
                     QERGIQTSVHYHPVHLFAHYRHVAERLPITESVAARTITLPLYPRLQESDVDYVAAAL
                     QDAIG"
     gene            1414..2004
                     /locus_tag="VGB88_04720"
     CDS             1414..2004
                     /locus_tag="VGB88_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX9646778.1"
                     /translation="MAAILIVGASHEALALARELGREVIAMADPKYGGDTFHGVPALA
                     SDVRALERGGFEAVLLAIDDPAARRAAYGFYGGRGIPACDLVAGHVGEGTGHGQGLFV
                     QRSAGISTDCRLGDGVRLNVGANVMHDCTLGDFVTVGPNAVVLGRVRIGRASYVGANA
                     TVLPGVAIGDGCVIGAGAVVTGDVPDNAVVKGNPAR"
     gene            2001..3020
                     /locus_tag="VGB88_04725"
     CDS             2001..3020
                     /locus_tag="VGB88_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646779.1"
                     /translation="MSGPAAKLDVLFVVDGSSATGFGHVARCLELYRLVRAARGSATA
                     AFQGSFSAPIRARIAAAAPELELAGPAAGRAARVCFVDRMADPDDPEACDRTQLDALR
                     ASCDRLVYMASGLTAPILPPGVACVGYQPGEPKAARPGLFWDLCYAPVRESVRRLAGR
                     AGEPGRALVAIGGSADLGPVRWAIRALAREPRVRAVDVLVSPAWAADASAQPSDGATV
                     HWHRAVPAEDLLAHAGLVVASQGNLGYEAMALGRAVVLVGVKDFQATIARRLAADGLA
                     VYGGHWPAATEQALAAAIGATIESAPRLVARQRQRLGDAGLHHVADLLRAMLDEVDVA
                     CASSP"
     gene            3002..3706
                     /locus_tag="VGB88_04730"
     CDS             3002..3706
                     /locus_tag="VGB88_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9646780.1"
                     /translation="MRVVAVIQARMGSSRLPGKVLAPLAGEPLLKRLIARVRAARRLD
                     GLIVATSQAPADDAVAAVCAATGTACFRGSETDVLGRFIAAADSLDADIAVRLTGDNP
                     AVDASLVDYVLDRALPHMPPAVYVHNVDDSGFPRGLYVEAATMAALRTAAGDADDTDR
                     EHVTWHLRRRPEAFPAVVVKAPGRFAEVSLTVDTHEDLRRLNDIFRSLYRSAPDFDFR
                     AFMVAADPAAAMDAKP"
     gene            3703..4713
                     /locus_tag="VGB88_04735"
     CDS             3703..4713
                     /locus_tag="VGB88_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX9646781.1"
                     /translation="MSTPFAIDGRAIGAGAPPYLVAEIGINHGGDADVAIGLVRAAKE
                     AGADAAKLQAFVPGELLARSSRYFDLLAGCALDQEAVRALAAAAKDIGLPLFSAAFDE
                     PSVDLLAALDVPAFKVASGDITHLPLLRHIGELGKPVILSTGGASIGEIEAALAALGA
                     GPGSVPVALLHCVSHYPTRPEEANLACIATMKAQFGLPVGFSDHTLGAATAIAAVALG
                     ADVIEKHFTFDRAAEGPDHALSAEPEELARIALGMQAAWRAIGRPAKAPVESGEHIAQ
                     IRRSVTARTPITKGTRLTAAMLAVKRPGTGIAPGRLDDVIGRRARRDLDADQTLSWDL
                     LD"
     gene            4726..5370
                     /gene="hisH"
                     /locus_tag="VGB88_04740"
     CDS             4726..5370
                     /gene="hisH"
                     /locus_tag="VGB88_04740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9646782.1"
                     /translation="MATRVAVIEYGVGNVGSVVNACARIGADVAIARDGDELRAQAPE
                     AIILPGVGAFGAALELIRSRGFEAAVGEQVHDAGVPFLGICVGMQALADSCEEFGEHH
                     GLGWIPGTVCRLVPPGSPVRLPHVGWNTVDASADDPVLGGLAGAHFYFVHSYAMRCDQ
                     QYVVGTTEYGGTFPSAVRIGHAWGIQFHAEKSSLAGERLLGQFLAFADACSSGG"
     gene            5400..6143
                     /locus_tag="VGB88_04745"
     CDS             5400..6143
                     /locus_tag="VGB88_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX9646783.1"
                     /translation="MVRSERFAVHQVIGNPVVHVERMVEWDVDELIVLDISEGEPTRF
                     HHERADYREPGAGDLLGFVRKIAAECRIPLTLGGRIRTVDDIAVRIRTGADKVTINTA
                     ALESPRLVGEAASRFGSQAIVVGIDYRRGADGARVYADHGRIDSGRNVVSWAREAADR
                     GAGEIFLNAVDRDGTAQGYDLESIARVADAVPVPVVACGGAGHMRHFLECFERTGASG
                     VAAGNIFHFTENAYPRAKAFLRGKRDDVR"
     gene            6147..7286
                     /locus_tag="VGB88_04750"
     CDS             6147..7286
                     /locus_tag="VGB88_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014243070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEX9646784.1"
                     /translation="MTHLNLPPIHWCARCVYPSSAAVTLVFDDAGVCSGCRVHDQKAG
                     IDWDQRLQWLLDEVEPYRRSTGYECVIGVSGGKDSYYQAHFVKEKLGLRPLLVTYDGG
                     NYLDVGWRNLMRMKEVFAADHIIVSPSVDLLMRMNRLAFRKMGDMNWQNHAGIATVPM
                     RVAVQHGIPLVFWGEHGWTDLGGMHSMHDMVEYTARYRKDQLLRGYDWYDMTGDDEDP
                     IGEGELECFKYPRDEEIDRIGLRGLFIGNYDPWDANAHAALVMERYGWQPSPVPFERT
                     YRRMSNLDDRYENGVHDYLKYVKFGYGRATDHACKDIRRGYMTRDQGIDMVRKYDHVR
                     SADLFHWLDYVGRSEDWFERIADGFRSPKVWVRSNGEWHKRNIWD"
     gene            7303..8313
                     /locus_tag="VGB88_04755"
     CDS             7303..8313
                     /locus_tag="VGB88_04755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9646785.1"
                     /translation="MTGHGLSRESIRPAAVMAGQRLAMRRDIDWLANRAGDFAPARCP
                     ACDADAPAALYEKYGMRHVRCGACATQYVSPRPDEATLARFYAESENYAYWAEHVFPA
                     SAEARRERIFRPRAATAASLVRRHGVDGATLVEIGAGFGLFCDEAAGTGAFARIIAVE
                     PTPQLARVCRERGLDTIAAPYEQIELDAPADLIAAFEVIEHLHDPARFLRWCHATLRP
                     GGGVLLTCPNIRGFETLILGRESDAVDHEHLNLFHPHSLRTLAERCGFAEVAVTTPGE
                     LDVDLVRDAVADRRIDAEALGPFLAAVIRDGDDAERATLQSFVRDAGYSSNMMMIARR
                     PQ"
BASE COUNT         1156 a         3021 c         2993 g         1263 t
ORIGIN      
        1 gcgtgcgacc gcatcacgcc gccctcggtc gagcgctggg tggtggcgac caccgccgac
       61 cccgagcagg cctatgcgcg catcgcggcg ctggtgcccg agtccaaggt cgcctacccc
      121 gacctgctcg agccggtcct ccagcgcgcc cgctcgcgcg gcacggggtg aggcgcccga
      181 gacggcatgg agcgtaaccc accccagctc tcctgcgccg acccggctct cgcctccggc
      241 gcggcgagcc ggccggccgc ggagctgcgt cgactcaacg tgttcgagcc cgacttcggg
      301 atcgaggagg agcaggcgct ggtcgcggtg ctgcgctcgg gctgggtggc gatgggcccc
      361 aaggtcgaag cgttcgagcg gcgcttcgcc gatctggtag gcacccaaca cgcggtcgcg
      421 gtcagctcgt gcacggcggc gcttcatctg actcaggtcg cgctcggcat cgggcgcggg
      481 gacgaggtga tcgtgccgtc gctgaccttc tcggcgaccg ccaactcggc gctggtcacc
      541 ggcgccaccc cggtgttcgc cgacatcgcc agcgccgacg actggaccct cgacccggcc
      601 gacgcggcgc ggcgcatcac cccgcgcacc aaggcggtga tcgcggtcca ctatggcggc
      661 catccggcgg acatggcggc gctcggcgcg gtctgccgcg acgccggcgt gacgttgatc
      721 gaggacgcgt gtcacgggct cggcggatcg ctcgacgggc gcgccatggg cgcactcggc
      781 gcggccggat gcttcagctt ctacagcaac aaggtgatca ccaccggcga gggcggcatg
      841 gtgacgaccg acgatgcgga cctcgccctc cacatcgcca agctgcgctc ccacggcatg
      901 accgcgaccg cgatcgaccg cgtgcgcggc gcgttcgact acgaggtcac cgaagccggc
      961 ttcaactacc gcctcgacga tctgcgcgcg gcggtggggc tcgcgcagct cgatcgcctc
     1021 gccgtcaagc tcgcccgccg gcgcgacctc gtgcgcctgt attgcgcgcg ccttgcgtcg
     1081 atacccgggc tgagcgtccc taatttcggc ggccggggcg agcccgccaa ctacctgatg
     1141 acggtgctgc tcgacggcgg cgaccgcgag gcggtgcgcc ggcgccttca ggagcgcggc
     1201 atccagacct cggtgcatta ccacccggtc catctgttcg cgcactaccg gcatgtcgcc
     1261 gagcgcctgc cgatcaccga atcggttgcc gcgcgaacca tcaccttgcc cctgtatccg
     1321 cgcctgcagg agtccgacgt cgactacgtc gcggccgcct tgcaggacgc gatcgggtag
     1381 ccgggcgcgg ggcggtcggg tggcggctgg tcgatggcgg cgatcctgat tgtcggcgcg
     1441 agccacgagg cgctggccct ggcgcgcgag ctcggccgcg aggtgatcgc gatggcggac
     1501 ccgaaatatg gcggcgatac gttccacggc gtgcccgcgc tcgccagcga tgtgcgtgcg
     1561 ctcgagcgcg gcggcttcga ggcagtgctg ctcgcgatcg acgaccccgc ggcgcggcgc
     1621 gcggcctacg gcttctacgg cgggcgcggc atcccggcgt gcgacctggt ggcgggccat
     1681 gtcggcgaag gcaccggaca cggccaaggc ctgttcgtgc agcgctcggc cggcatctcc
     1741 accgactgcc ggctcggcga cggcgtgcgg ctcaacgtcg gcgccaacgt catgcacgac
     1801 tgcacgctcg gcgatttcgt caccgtcggg cccaacgccg tcgtcctcgg ccgggtgcgg
     1861 atcggcaggg cgagctatgt cggcgccaat gccaccgtgc tgcccggcgt cgccatcggc
     1921 gacggctgcg tgatcggcgc cggcgcggtg gtcacgggcg acgttcccga caacgccgtg
     1981 gtcaagggca atccggcgcg atgagcggac cggcggccaa gctcgacgtc ctgttcgtgg
     2041 tcgacggctc gtccgcgacc ggcttcggcc atgtcgcgcg ctgcctggag ctgtatcgcc
     2101 tggtgcgggc cgcgcgcggg tccgcgaccg cggcgttcca gggcagcttc tcggcgccaa
     2161 tccgcgcgcg catcgccgcc gcggcaccgg agctcgagct cgccggcccg gccgccggcc
     2221 gcgccgcgcg ggtctgcttc gtcgaccgca tggcggaccc ggacgatccc gaggcctgcg
     2281 accgcacgca gctcgatgcg ttgcgcgcct cgtgcgaccg gctggtctac atggcctcgg
     2341 ggctcaccgc gccgatcctg ccgcccggcg tcgcgtgcgt tggctaccag cccggcgagc
     2401 ccaaggccgc gcgcccgggc ctgttctggg acctgtgcta tgcaccggtg cgcgagtccg
     2461 tccggcgcct cgccgggcgc gccggcgagc ccggacgcgc tttggtcgcg atcggcggca
     2521 gtgccgatct cgggccagtc cgctgggcca tccgggcgct cgcccgcgag ccgagggtgc
     2581 gcgcggtcga cgtgctcgtc tcgcccgctt gggcggcgga cgcctcggcg cagccgtccg
     2641 acggcgccac cgtgcattgg caccgggcgg taccggccga ggacctgctg gcgcacgccg
     2701 ggctggtggt cgcgagccag ggcaacctcg gctacgaggc gatggcgctg ggccgcgcgg
     2761 tcgtgctggt cggcgtcaag gacttccagg cgaccatcgc ccggcggctg gcggcggacg
     2821 gtctcgcggt ctatggcggg cattggccgg cggcgacgga acaggcgctc gcggcggcga
     2881 tcggcgccac catcgagtcg gcgccgcggc tggtcgcccg ccagcgccaa cggctcggcg
     2941 acgccggctt gcatcacgtc gccgacctgt tgcgtgcgat gctcgatgag gtggacgtgg
     3001 catgcgcgtc gtcgccgtga tccaggcgcg catgggctcg tcgcgcctgc cgggcaaggt
     3061 gctggcgccg ctcgccgggg agccgctgct caagcgcctg atcgcgcgcg tccgcgcggc
     3121 ccggcggctc gacggcctga tcgtcgccac ctcgcaggcg ccggccgacg acgcggtcgc
     3181 cgcggtgtgt gccgccacgg gcaccgcctg cttccgcggc agcgagaccg atgtgctcgg
     3241 ccggttcatc gccgccgccg acagcctcga cgccgacatc gcggtccggc tcaccggcga
     3301 caaccccgcg gtagacgcca gcctggtcga ctacgtgctc gaccgggccc tgccgcacat
     3361 gccgccggcg gtctatgtcc acaatgtcga cgacagcggc ttcccgcgcg gactttatgt
     3421 cgaggcggcg acgatggcgg cgctgcgcac cgccgcgggc gacgccgacg acaccgaccg
     3481 ggagcacgtc acctggcacc tgcgccgccg gcccgaggcg tttcccgccg tcgtggtcaa
     3541 ggcgccgggg cggttcgccg aggtctcgct caccgtcgac acccatgagg acctgcggcg
     3601 tttgaacgat atcttccgca gcctgtaccg gtcggccccc gatttcgact tccgggcgtt
     3661 tatggttgcg gccgatccgg ccgcagcgat ggacgcgaag ccgtgagcac gccattcgcc
     3721 atcgacggcc gggccatcgg cgccggcgcg ccgccctatc tggtcgccga gatcggcatt
     3781 aaccacggcg gcgacgccga cgtcgcgatc gggctggtcc gcgccgccaa agaggccggc
     3841 gccgatgccg ccaagctgca ggcattcgtg ccgggcgagc tgctcgcccg ttccagccgc
     3901 tacttcgacc tgctcgccgg ttgcgccctc gaccaggagg ccgtgcgcgc gctggcggcg
     3961 gcggcgaagg atatcgggct gccgcttttc tcggcggcgt tcgacgagcc cagcgtcgat
     4021 ctgttggcgg cactcgacgt accggcgttc aaggtcgcgt ccggcgacat cacccatctg
     4081 ccgctgctgc gccatatcgg cgagctcggc aagccggtga tcctgtcgac cggcggcgcc
     4141 agcatcggcg agatcgaggc ggcgctggcg gcgctcggtg ccggcccggg gtcggtgccg
     4201 gtggcgctgc tccattgcgt gtcgcactat ccgactcggc ccgaggaggc gaacctcgcc
     4261 tgcatcgcga ccatgaaggc gcagttcggc ctgccggtcg gcttctccga ccacacgctc
     4321 ggcgcggcga ccgcgatcgc cgcggtcgcg ctcggcgccg acgtcatcga gaagcatttc
     4381 accttcgacc gcgccgccga gggaccggat catgccttgt ccgccgagcc cgaggagctt
     4441 gcgcgcattg ccctgggcat gcaggcggcc tggcgggcga tcgggcggcc ggcgaaggca
     4501 ccggtcgaga gcggcgagca catcgcccag atccggcgca gcgtgaccgc gcgaacgccg
     4561 atcacgaaag ggacccgcct gaccgcggcg atgctcgccg tcaagcgccc cggcacgggc
     4621 atcgcgccgg ggcgcctcga cgacgtgatc ggccgccggg cgcggcgcga tctcgatgcc
     4681 gaccagacgc tgagttggga cctgctggac tgagcgcggg cagcgatggc gaccagggta
     4741 gcggtgatcg agtacggcgt cggcaatgtc ggatcggtgg tcaatgcgtg tgcgcgcatc
     4801 ggcgccgatg tcgccatcgc ccgagacggg gacgagcttc gcgcccaggc gccggaagcg
     4861 atcatcctgc cgggcgtcgg cgcgttcggc gccgcgctcg agctgatccg cagccgcggc
     4921 ttcgaggcgg cggtgggcga gcaggtgcac gatgcgggcg tgccgttcct cggcatttgc
     4981 gtcggcatgc aggcgctcgc cgactcgtgc gaggagttcg gcgagcacca tgggctcggt
     5041 tggatcccgg gcacggtgtg ccggctggtg ccgccgggct cgccggtgcg cctgccccat
     5101 gtcggctgga acaccgtcga tgcgtcggcc gacgatccgg tgctcggcgg gctcgccggc
     5161 gcgcatttct acttcgtcca ctcctatgcc atgcgctgcg atcagcaata cgtggtcggc
     5221 accaccgagt acggcgggac cttcccctcg gcggtgcgca tcgggcacgc atggggcatt
     5281 cagttccacg ccgagaagag ctcgctcgcc ggcgaacgat tgctgggcca gtttcttgct
     5341 ttcgctgacg cgtgctcaag cggcggctga taccggtcct gtatcttcgg catggctgga
     5401 tggtgcgcag cgagaggttt gccgtccacc aggtcatcgg caatcccgtg gtacatgtcg
     5461 agcggatggt cgagtgggac gtggacgagc tgatcgtgct cgacatcagc gaaggcgagc
     5521 cgacccggtt ccaccacgag cgcgcggact atcgcgagcc cggtgcgggc gacctgctcg
     5581 gcttcgtgcg caagatcgcg gccgaatgcc ggattccgct caccttgggc gggcgaattc
     5641 gcacggtcga cgacatcgcc gtgcgcatcc gcaccggcgc cgacaaggtg acgatcaaca
     5701 ccgccgcgct cgagtcgccg cggctggtgg gcgaggccgc gtccagattc ggcagccagg
     5761 cgatcgtggt cggcatcgac taccggcggg gcgccgacgg ggcgcgggtg tacgccgacc
     5821 acggccgcat cgacagcggt cgcaatgtcg tctcctgggc gcgcgaggcc gccgatcgcg
     5881 gcgccggcga gatcttcctc aatgccgtcg accgcgacgg caccgcgcag ggctacgatc
     5941 tcgaatcgat cgcccgcgtc gccgacgcgg tgccggtccc ggtggtcgcc tgcggcggcg
     6001 ccgggcacat gcgccacttc ctcgaatgct tcgagcgcac cggcgcgagc ggcgtcgccg
     6061 ccggcaacat ctttcacttc accgagaacg cttacccgcg cgccaaggcg ttcctgcgcg
     6121 gcaagcgcga cgacgttcgc taagccatga ctcacctcaa cctgccgccg atccactggt
     6181 gcgcgcgctg cgtctatccg tcgagcgcgg ccgtgacgct ggtgttcgac gacgccggcg
     6241 tgtgcagcgg ctgccgggtc cacgaccaga aggccgggat cgactgggac cagcgcctgc
     6301 aatggctgct cgacgaggtg gagccctatc gccgctcgac cggttacgag tgcgttatcg
     6361 gcgtctccgg cggcaaggac agctactacc aggcccactt cgtcaaggag aagctcgggc
     6421 tcaggccgct gctggtcacc tatgacggcg gcaactacct cgacgtcggc tggcggaacc
     6481 tgatgcgcat gaaggaggtg ttcgccgccg accacatcat cgtctcgcct tcggtcgacc
     6541 tgctgatgcg catgaaccgg ctggcgttcc gcaagatggg cgatatgaat tggcagaacc
     6601 atgccggcat cgccacggtg cccatgcgcg tcgcggtcca gcacggcatc ccgctggtgt
     6661 tctggggcga gcacggctgg accgacctcg gcggcatgca ctcgatgcac gacatggtcg
     6721 agtacaccgc gcgctaccgg aaggaccagc tgctgcgcgg ctacgactgg tacgacatga
     6781 ccggcgacga cgaggacccg atcggcgagg gcgagctcga atgcttcaag tatccgcgcg
     6841 atgaggagat agaccggatc ggcttgcgcg ggctgttcat cggcaactac gacccgtggg
     6901 acgccaacgc ccatgccgcg ctggttatgg agcgatacgg ctggcagccg tcgccggtgc
     6961 cgttcgagcg cacctatcgg cgcatgtcga acctcgacga ccgctacgaa aacggggtcc
     7021 acgactatct gaaatacgtc aagttcggct acggccgcgc caccgaccat gcctgcaagg
     7081 acattcgcag gggctacatg acccgcgacc agggcatcga catggtccgc aagtacgatc
     7141 acgtacgctc cgccgacctc tttcactggc tcgactacgt cgggcggagt gaggattggt
     7201 tcgagcggat tgccgacggc ttccgcagcc ccaaggtctg ggtccgctca aacggcgaat
     7261 ggcacaagcg caacatctgg gactaggcgc cgcgggcgca cgatgaccgg ccacggcctg
     7321 tccagggaga gcatccgccc ggccgccgtc atggccggcc agcggctcgc catgcgccgc
     7381 gacatcgatt ggctggctaa cagggccggc gacttcgcgc ccgcgcgctg tcccgcctgc
     7441 gacgccgatg cgccggcggc gctgtacgag aagtacggca tgcgccacgt gcgctgcggc
     7501 gcctgcgcca cccaatatgt gtcgccgcgc ccggacgagg ccacgctcgc tcgcttctac
     7561 gccgaatccg agaactacgc ctactgggcg gagcacgtgt ttcctgcgag cgccgaagcg
     7621 cgacgcgagc gcatcttccg cccgcgcgcg gcgaccgcgg caagcctggt ccgccgccat
     7681 ggcgtggacg gcgccacgct ggtcgagatc ggcgccggct tcgggctgtt ctgcgacgag
     7741 gccgccggca ccggcgcgtt cgcgcgcatc atcgccgtcg agccgacccc gcagctcgcc
     7801 cgggtctgtc gcgaacgcgg tctcgacacg atcgcggccc cctatgagca gatcgagctc
     7861 gacgcgcccg ccgacctgat cgccgcgttc gaggtgatcg agcaccttca cgacccggcg
     7921 cggttcctcc gctggtgcca tgcgacgttg cggccgggcg gaggcgtgct gctcacctgt
     7981 cccaacatcc gcggcttcga aacactgatt ctcggccgcg agtcggacgc cgtcgaccac
     8041 gagcacctga acctgtttca tccccactcg ctcaggacgc tcgccgagcg gtgcgggttt
     8101 gcggaggttg cggtgacgac gccgggcgag ctcgacgtgg atctggtccg cgacgcggtc
     8161 gccgaccggc gcatcgacgc tgaggccctg ggcccgttcc ttgccgcggt gatccgcgac
     8221 ggcgacgacg cggagcgcgc tacgctccag tcgttcgtgc gcgacgcggg ctactcgtcc
     8281 aacatgatga tgatcgcacg gcggccgcag tgatcgcggt cttcctgacc ggcaaactga
     8341 tctccaccga cacgatcctg ccggtgatga tggcggtgaa gcggctcgcg ccgcgccagc
     8401 ggatcgtgtt ctacctcgcc agccggcgcg cgc
//
LOCUS       DASWHU010000133        30572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2284548, whole genome shotgun sequence.
ACCESSION   DASWHU010000133 DASWHU010000000
VERSION     DASWHU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 30572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30572
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284548"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VGB88_04760"
     CDS             complement(<1..854)
                     /locus_tag="VGB88_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9646786.1"
                     /translation="MQIKDLKTYVVGNPPPHRGGPCWVFLKLTTDGGIDGFGEAYGVP
                     FDPHLVARMIEDVVARHVAGTDPFAIERLWRIVYSRGFTQHPDHALMGVLSGIEMACW
                     DIIGKALDQPVYNLIGGLVNERIRSYTYLYPSPEELEGAEDALDIANAFGDAERAPAR
                     AAAYLAQGFTAVKFDPVMPMSAFDPRQLSLESLDNAALVTRNLREAVGGACDLLVGTH
                     GQMTTSSAIRLAKRLEPYDPLWLEEPVPPENMAEMARVARATTIPIATGERLSTKYEF
                     AELLERQAA"
     gene            complement(889..1686)
                     /locus_tag="VGB88_04765"
     CDS             complement(889..1686)
                     /locus_tag="VGB88_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9646787.1"
                     /translation="MIEVPDDPGLDGKVAIVAGGGAEGEGIGNGRAAAILFARAGARV
                     LVVDLKRDLAERTVAMIDSFGGTAEAFAADVTKEDDCARMVAHALERFGRLDILDNNV
                     GIAGQATVVEETEARWERLMRVNVTAMFLACKHAIPAMIAAGGGAIVNISSISALRPR
                     GLTAYSTSKGAVIALTRAMAVDHAGDGIRVNCVAPGPVYTPMVYAGGMSAESRERRRM
                     AAPMEVEGSGWDVGNAVVFLASARARFITGQTLVVDGGATLVGRPRA"
     gene            complement(1683..2522)
                     /locus_tag="VGB88_04770"
     CDS             complement(1683..2522)
                     /locus_tag="VGB88_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9646788.1"
                     /translation="MPTENGPEHAPADRYGAFDTLTFDRPAARVLRVTMGGGERLSSV
                     DARAHAQLAELWRAIDADPEVSAVILRGAGQGFSAGGDFALIERIAEDYDTRVRVWKE
                     ARDLVYNVINCSKPIVSAMHGAAVGAGLAAGLLADISIAARDARIIDGHTRLGVAAGD
                     HAAIIWPLLCGMAKAKYYLLLCEPLSGEEAERIGLVSLAVDRAALDATALDVASRLAA
                     GAPTALRWTKYALNNWLRAAGPAFDASLALESLGFTGPDVREGVASLRAKRAPRFKPE
                     TPL"
     gene            complement(2608..3129)
                     /locus_tag="VGB88_04775"
     CDS             complement(2608..3129)
                     /locus_tag="VGB88_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9646789.1"
                     /translation="MTDRHLALLRHAKSSWQLGPERDHERPLDERGQRAAAVVGTYIA
                     QSGLVPDLVLCSTAKRARETLDAVLARLDARPEIRFERALYLAPAEAIGARLRRVGGG
                     WREVLVVGHNPGLQAFAAGLARLADPGRAARITDKFPTCALVRFSLELTRWRELRPEA
                     VRAVELVVPKDLV"
     gene            complement(3264..3686)
                     /locus_tag="VGB88_04780"
     CDS             complement(3264..3686)
                     /locus_tag="VGB88_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9646790.1"
                     /translation="MTTHLMTLVSRLLVRSLSQRLGPKGIGYGQYPVLLQLWEEDGLT
                     QKELSNRVRIEAPTMVRTLDRMEREKLVNRKRSDADRRQIHIQLTPKGRALKGQLGSL
                     SGQVDRIALSGMTRAERVRLDALIGRVVRNLEADSEAA"
     gene            3793..4308
                     /locus_tag="VGB88_04785"
     CDS             3793..4308
                     /locus_tag="VGB88_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646791.1"
                     /translation="MAVVRLPADRLAVPRRAVVRLAVVRLAVARLAAARFGAARLVAV
                     RLAVVRLVAARFGAARFAVVRLVAARFGAARFAVFRFGAALARLAVVLAPAFLGAARR
                     VAFLAVVLARAPAERVDFARVVPAFAALVRVVLVFAVLAFGAAAFLLAAMEINSPSRL
                     DGMPTPPSFRL"
     gene            complement(4614..5453)
                     /locus_tag="VGB88_04790"
     CDS             complement(4614..5453)
                     /locus_tag="VGB88_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646792.1"
                     /translation="MTATAGAPSNRAEPPLLSVNSIEVVYDHVILVLKGVSLSLPAGD
                     VVTLLGANGAGKSTTLKAISNLLRAERGEVTKGSISFDGERVDRLTANELVRRGVIQV
                     MEGRRCFQHLTVEENLLTGAYTRRDGRDRIGRDLDLVYSYFPRLKERRSGLAGYISGG
                     EQQMTAIGRALMARPRLILLDEPSMGLAPQLVEEIFEIVRRLNRDEGVSFLLAEQNAT
                     IALRYADFGYILENGRVVLDGDAAVLRENEDVKEFYLGLSESGRKSYRDVKHYRRRKR
                     WLA"
     gene            complement(5479..6822)
                     /locus_tag="VGB88_04795"
     CDS             complement(5479..6822)
                     /locus_tag="VGB88_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646793.1"
                     /translation="MGFRSNASALAAAAMVLSFGSAAFAQDESDMQFYPVNAYWTGPY
                     AAGGSAFGGGMIDYLKMLNERDGGIYGVKLFWEKCETQYQNDRIVECYERMKNRGAGM
                     PFFHPMSTGGTYAVFERAWKDEIPVLSVGYGRTDASDGRVFPYLFPMLTNYWSQNTAK
                     IRFIGMREGGMDQLKGKKIANVYHDSAYGKETIPILDKQAGIYGFEVRHYPVAHPGLD
                     QSAIWLQIARQYKPDWVILRGWGVMNPTALKEAQRIGFPRDHMVGVWWSGAEEDTVPA
                     GDAAEGYIAAGFHPSGTDFPVIQDILKYVYDKGNGELPRERVGSIYYNRAIVWAIVST
                     EALRLAMDMVGHRPVTGKEVQQALDRLALDKEHIKALGADGLIPPFALSCADHEGGAA
                     VQFQQWTDGKWVQISDWIPTDQELVRPMVEDSAAQYAKEKGISLRECQQTQAEVR"
     gene            complement(6925..7989)
                     /locus_tag="VGB88_04800"
     CDS             complement(6925..7989)
                     /locus_tag="VGB88_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9646794.1"
                     /translation="MIYRQAGQFKTSYAADQAIFPIRQDLWFVLGILAAAVVAVPLLA
                     SEYLLHSVLLPFLILSVAAMGLNILLGYCGQISLGTAGFMAVGAFSAYKLATAFPALP
                     FLATFFVAGLITALVGVFFGLPSLRIKGFYLAVTTLGAHFFIEWTITHFGWFSNYSAS
                     GVISAPAITLLGFPIESAQSRYLVTVLIVIGLALVMKNLVRSAIGRAWMAVRDMDVAA
                     QVIGIRLLRTKLLAFAVSSFYCGVAGAMYVFLYLGTVEPQGFEINRAFQVLFMVIIGG
                     LGSVMGSFFGAAFIVMLPILITNIAGQLGSVMPAEVLSNLELIVFGSLIVLFLIVEPA
                     GLARLWSIAKEKLRLWPFPY"
     gene            complement(7993..8934)
                     /locus_tag="VGB88_04805"
     CDS             complement(7993..8934)
                     /locus_tag="VGB88_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9646795.1"
                     /translation="MLDVAFSDPLFAVEVLVRGLLSGIMYSLVALGFALIYKASGVFN
                     FAQGAFVLFAALTFVSFLEMGLFGASGDLWYFGVCVVIALAVMVVYGVAVERFVLRPL
                     VNQPLIILFMATIGLNFFTEGFAQSLWGAEVHALDLGIPDVPWQEFGEATGIYLSVFE
                     LVAAGIAGVLVLGLSLFFSKTRTGRALRAVSDDHQAAMSVGIPLQHIWAITWAVAGII
                     ALIAGMLWGAKLGVQFSLSLLALKALPVLIIGGFDSIAGAIVGGLIVGASEKLAEVFI
                     GPYFGGALENWFPYVLATVFLLFRPQGLFGEKIIERV"
     gene            complement(8971..9762)
                     /locus_tag="VGB88_04810"
     CDS             complement(8971..9762)
                     /locus_tag="VGB88_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646796.1"
                     /translation="MSADGREIGDVLLSARDISIRFGAVVALAGVSVDIRSHEILAII
                     GPNGAGKTSLLNCINGVYHPQQGSIIFDGAKRRGASPAEIARQGIARTFQNVALFKGM
                     TTLDNIMTGRNTRAGGNILLQALYWGPYRKQELEHRRAVERIIDFLEIQSIRRTPVGR
                     LPYGLQKRVELGRALAAEPRLLLLDEPMAGMSVEEKEDMSRFILDVNDEFGTTIVLIE
                     HDMGVVMDISDRVVVLDYGRKIAEGTPDEVRRDQSVIDAYLGVSH"
     gene            complement(9763..11730)
                     /locus_tag="VGB88_04815"
     CDS             complement(9763..11730)
                     /locus_tag="VGB88_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9646797.1"
                     /translation="MDVPVHQGAMAALDTFPKLLIHQAEVRAPKAFLREKDYGIWQAL
                     SFADALKETRSLALGLAARGLERGDRVAIIGDNRPYLYLAIAAAQALGAVPVPIYQDS
                     IAAEMHYILDHAEVRFAVVEDQEQVDKLLQIRDRLERLELIVFHDPRGMRHYNEPGLA
                     SYQDVQRQGERLDRERPGLFMDEVAKGRGGDLAIMLYTSGTTGKPKGVMLTYDNLERT
                     SYNGIAYDKLTEADEVLAYLPMAWVGDHLFSYSQALCAGFTVNCPESGATVLADLREI
                     GPTYFFAPPRIWESILTSVMVRIEDAAAIKRRLFRFFIKLAERVGTRLLDGEPVALGD
                     RLLYWLGELLVYGPLKDNLGFSRIRVAYTAGEAIGPEIFAFYRSLGINIKQIYGMTEA
                     TALVCAQPDGEVSPDTVGTPYPGVELRVSEAGEVQFRGPGTFVGYYKNEQATAETKSA
                     DGWISSGDAGYIDDAGHLRIIDRAKDVGRLNDGTLLAPKFLENKLKFSPFILEAVAHG
                     HGRDFATAFINIDLESVGNWAERRGLAYASYTDLAARPEVYDLIAGEIGKVNAALAAD
                     PKLVGSQIRRFLILHKQLDPDDNELTRTRKVRRGYVGEKYADLIEALYGGAREVQVEA
                     RVTFENGREGTITAMLGIRDLEPVAQLAKAS"
     gene            complement(11899..12645)
                     /locus_tag="VGB88_04820"
     CDS             complement(11899..12645)
                     /locus_tag="VGB88_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX9646798.1"
                     /translation="MAAMIYLFTDFGGNGPYVGQVKAVLAAAAPGVPVIDLAHDAPVF
                     EHKASAYLLAALADRLPAAGVVLGVVDPGVGRTDRRPVVVRAGRFWYVGPDNGLFAVA
                     AKHAGDAAWWQITWRPERLSSTFHGRDLFAPVAAMLARGEDVPGRPIAAEGAAGMGWP
                     DDLGEVIYLDHYGNAMTGIRASTLPDDAILKIGDVRVQRARTFAEVGLGQAIWYENSC
                     GLVEVAMNRANAAAVMRLTVGDHVLAIALT"
     gene            12677..14038
                     /locus_tag="VGB88_04825"
     CDS             12677..14038
                     /locus_tag="VGB88_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9646799.1"
                     /translation="MPHRSAQPGPRTAQAVELVVAALGARGDGIATHDGRPVYVAGAL
                     PGERVRARLGAARGDGRAAMLERVLAPAAERVAPACRHFGTCGGCAVQHLARAAELLW
                     KRGVIADALSRRGLADAPVAPARVVAPGKRRRATFVAARAGARVTLGFHQRRSHRLVD
                     IAECPAIMPELEALAAPLRDGLAGVLRDREQIKLHAQATETGIDLVIDAARAPQLAER
                     EALAGLAERLDLARLTWRADGELEPIAWRRPARVSFAGIAVEPPPGAFLQPSADGEAA
                     IRAAVLAAMGPARTVADLFAGVGTLALPLAAQATVAAVHAVDIAGDHLDALAKAARAL
                     DRARIGVEQRDLFAAPLAGPELDRFDAVVFDPPRAGARAQAAALAASAVPAVVAVSCD
                     PATFARDARLLVDGGYRLELVTPIDQFPWSAEVELVAAFRRPGSGAGARTRTRSRRPP
                     PAG"
     gene            complement(15348..16196)
                     /gene="ccsA"
                     /locus_tag="VGB88_04830"
                     /pseudo
     CDS             complement(15348..16196)
                     /gene="ccsA"
                     /locus_tag="VGB88_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
     gene            complement(16180..16878)
                     /locus_tag="VGB88_04835"
     CDS             complement(16180..16878)
                     /locus_tag="VGB88_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9646800.1"
                     /translation="MLVGLADSGVDDELLARVVAARGFTAGEGEQAQTAAPMPDPVGH
                     GSALARIVLAHAPAARIVNAQIFGARGATSAAVAAAAVAWLAEQGAAVVNLSFGLHED
                     RAPLRAACARALAAGCALIAAAPARGAPVFPSSYAGVIRVTGDARCGFAEVSHLATAQ
                     ADFGACVQVQAGAPGAGRIAGASVAVAHASGLVAARFAETGARGAEAARAVLAAAAVY
                     HGAERKARHDARNA"
     gene            complement(16928..18664)
                     /locus_tag="VGB88_04840"
     CDS             complement(16928..18664)
                     /locus_tag="VGB88_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646801.1"
                     /translation="MSEDIGADATTAADDRRTLAWAVSFLRPHARGLALVLALAVATT
                     AVTSAQPYIMKLMIDDGVLAGDLARLAWLTALMLGLGLGNLGFGGLNRYLHVKVSGRI
                     LFAMREAVFRHLQTLSPAFFARLRTGDVLQRLDGDVAQVQRFCVDSLLAGINGAFALA
                     AALALMLWLSWQLSLVALVLLPAEYAYLRVMRPRVESRARATRARASDLSAFLVETLP
                     ATKFVQSVGAEAREAARLEGLNRRYLGDLLRLQLAEFLTAAVPNLLTSASRAGVFLAG
                     GYLIVAGDLTLGTLIAFNTYLGFATGPVHSLLGIYVGMMRARVSLARVRALTDTAPLV
                     VSPPSPAALPATAAGAIRIDSVSFAYPGTDHAVLEHASLDIPAGTKLGVLGPSGAGKT
                     TLIDLLQRHFDPDLGRITLDGIDLRALDLAELRRRIAVVAQDVVLFRGTIADNVRYAA
                     PAATEDEVRTALRRAQLDQFVGELPAGADTMIGERGARLSGGQRQRLAIARALLQDPL
                     VLVLDEATSAVDLATEARLIAAVDALFAGRTRIVISHRAETLAGADRVVEAAGGRFRD
                     ARPARAPAPGHG"
     gene            complement(18779..19909)
                     /gene="peaD"
                     /locus_tag="VGB88_04845"
     CDS             complement(18779..19909)
                     /gene="peaD"
                     /locus_tag="VGB88_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     beta"
                     /protein_id="HEX9646802.1"
                     /translation="MNRLLRAASIALLAGAGLAALAATGALAKEYVIAVNKPNNLHVI
                     DADARKVVNSCTLPGRGSPVAIATSPVDPAVAFVETNGWGEVVGVNIDSCEVFFHAVL
                     SQGTVRARSMAGVAVGLDGKELFVQVAETELLADRYQIRPMHRFLVYNTDAGLDAKPA
                     RSFDVPRGQTIMAPSHDGKTLWSYGHEVRQFDIATGKILQEIRLNSWEEDKPTYGDPD
                     GLSFWPLYDNIDVMIFPYVAPVFASREARDAGDFDQLSDFVLGITTVDLRTNEWAQRD
                     ITSFDVLIFSMVTHPKKPSEIYAVYTTLSKYDLDTGKLVDRVDLDHTYYAAAISAEGD
                     ELYIGGTFNDVAIYSTDTLKQLAKVMLPGGDQGVAGIRVIQR"
     gene            complement(19947..20279)
                     /gene="qhpC"
                     /locus_tag="VGB88_04850"
     CDS             complement(19947..20279)
                     /gene="qhpC"
                     /locus_tag="VGB88_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     gamma"
                     /protein_id="HEX9646803.1"
                     /translation="MKHLKSFNRKAKLLESALEQGKADEVRMMQTVVAGCSSTLDPGW
                     EVDPFGGVAGLCQPMEADLYGCSDPCWWPAQVPDTINTYPDWNANAPSAKDWRVLESV
                     FPSEDGTV"
     gene            complement(20276..21724)
                     /gene="peaB"
                     /locus_tag="VGB88_04855"
     CDS             complement(20276..21724)
                     /gene="peaB"
                     /locus_tag="VGB88_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase maturation
                     protein"
                     /protein_id="HEX9646804.1"
                     /translation="MSELRLVEHNLHQVRVAGRRMMFHIPTSSLFELDAVSGDLLDLF
                     DAESSGARPAVTPELVRESFDGRHAPEAVVEAIRDFIELEVLSDGRARAPERAPIRIE
                     NFPLSTIVLNTNTGCNLSCTYCYKEDLETPANGTKMEFETARKSIELLLAEGAQRERF
                     NIVFFGGEPLSNMALIRAVVDYAEARASELGKKVDFTLTTNGTLLTERLVDYFNAHRF
                     GLTISMDGPRAVHDLRRRTVGGKGSYDVVAAKARMALERYDSRPIGARVTLTAGVVDV
                     VAIWDHLVNDIGFHEVGFAPVTSGEHAIFALNGDELAELFANMKTLGGIYLEAAFAHR
                     NIGFSNMHQLMTDLSEGMSKALPCGAGLGLLAVDTGGGLNLCHRFTGSGLDTFGDVDH
                     GIDKPALGRFLESRLDRSETACNTCRIRNLCSGGCYHESYARYNDPIHPTYHYCDLMR
                     DWVDFGIGVYTRIMERNPAFFATYVEPRRAYK"
     gene            complement(21760..23364)
                     /gene="peaA"
                     /locus_tag="VGB88_04860"
     CDS             complement(21760..23364)
                     /gene="peaA"
                     /locus_tag="VGB88_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     alpha"
                     /protein_id="HEX9646805.1"
                     /translation="MSVRDWAFGWLVGAVALIALVLSTDGVRAADGEAILNARCGACH
                     VDEGDGLSRIKGQRKTPEGWLMTIVRMGIMHGVEVPADERRALVKYLADSQGLAPSET
                     TGYRYILEREPAQIEDFPPEYFYMCARCHSGARGALQRRTKDEWRWHIDFHLGQWPTT
                     EYQFYGRDREWYRIASTETVDWLYEHYPFDSQAWQDWKGHSVEMSGDWRWVGRGPGVG
                     FHNGITTFSATGEDTYSASDSEFNLDGSAVEASGSAIIYTGYELRARLTEATSGDEYL
                     SIAALSEDGNRAEGRFMLSAHDETGAHFVAVRMRDGHSEIMAVEPAHIRAGSEAEIAI
                     HGTGLNGAVDLGAGVEVVNTVSSGSTMVVVNAKAAADAALGARAIKVGDAASANDALV
                     VYDAVARVAVEPAYAFARVGEGGGTRPDQYALFDAVGFTAGPDGAAGTDDDVRIGVMP
                     ATWSVEPFDEVAAELDDVGFAGQMDPKTGIFDPADAGLNPARPFETNNAGNLKVIGTV
                     DDAGNAVAGEGQLLVTVQRWNDPPIR"
     gene            complement(23498..24874)
                     /locus_tag="VGB88_04865"
     CDS             complement(23498..24874)
                     /locus_tag="VGB88_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9646806.1"
                     /translation="MARAEVVIVGGGVAGAVAAAALARAGRRVHLITSRRRQSLIEGR
                     SERAADGFRFAGCTRALDVAGPWVERFSHWNGRRVQANGETLVDRARLDAALLDDARA
                     AGAAVTVARIAALLPIKGGWRALARGGVEVEAALLIEARGRQAPAGTARRRHGPPATA
                     LVRRLRLARAIAPQSTVASFADGWLWLAAPGDGTAAVQVVVAGEALPPRRALEAFFSE
                     RIAGVPEVAELIAGAGPAGPLVARNADAVLCAPACGPGLIRIGDAAVAIDPLSGHGQF
                     TAASTALNAVAVANTLLDRAGDRALAERFYGARIEETFLVQARVGRDFYRLERRWAAR
                     PFWAARTPWPDDAPAHPRPDAAPARIARLPVIDGGYVSEREVVVTADHARGVWQVDGV
                     ALVPLIRFLAGGWQDGGGLAAAAAAHLRCPRARVETALAWLARRGLLAARPMHPVADA
                     GPRGGASA"
     gene            complement(24903..25331)
                     /locus_tag="VGB88_04870"
     CDS             complement(24903..25331)
                     /locus_tag="VGB88_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9646807.1"
                     /translation="MLVREILKTKGTQVFTITRDQSIAELAKELVERRIGAAVVVDGE
                     GALIGVISERDIVYGVADEGSACLDRKVEDLMTADVISCTPDTTIVDVMNQMTERRIR
                     HLPVLEDGRLAGLVSIGDVVKQRIASAEREADQLREYISA"
     gene            complement(25477..27510)
                     /locus_tag="VGB88_04875"
     CDS             complement(25477..27510)
                     /locus_tag="VGB88_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9646808.1"
                     /translation="MTTKAPSAPRSVALVGPFSGGKTSLLENLLFAAGAISRKGTVKD
                     GNMVGDGSTEARARQMSVEVSVASFEFMGESFSVIDCPGSLEFLQEALDALVGVDAAI
                     VVVEPEIDRLVALAPVLNFLDARAIPYMVFISKMDRAAAPVKDLLAAMQAQAGRPLVL
                     RQVPIRDGETLTGYIDLVHERAFRYQADAASALIRIPEPMAADEKAARTELLESLADF
                     DDTLLEQLLEDVIPPNEEIFQAMSKDFGAGLVAPVLLGAGEHEHGVRRLLKALRHDLP
                     EAQSTAERVGVAGGEGTLAQVLKTYNTATSGKLSLVRVWRGTLTDGMTLNGQRVGSVN
                     KLLGQATTKVAKAVAGEVVALGRMDEVGTGDMLSSAGAAKVPDALLVAPLKPVYAFGI
                     AAEKRADEVKLSDALGKLLEEDRSLVAEHDPDTHQHLLRGQGEMHLKVALDRLRNKFN
                     LPLVTEPVRVPYKETIRKRAERVQGRHKKQTGGHGQFGDVYITVEPRPRGSGFEFKDS
                     ITGGVVPKQYIPAVETGVVDFCKKGPLGFPVVDLSVKLVFGSYHTVDSSEMAFQTAGR
                     IAMQAALPQCNPVLLEPIYRVTVFVPNEFTAQVQRLLSTRRGQILGFETRPGWQGWDQ
                     VSAQLAQAEMNDLIIELRSLTRGVGSYDYQFDHMQELSGKLADDVVAQAAAAE"
     gene            complement(27695..28537)
                     /gene="soxA"
                     /locus_tag="VGB88_04880"
     CDS             complement(27695..28537)
                     /gene="soxA"
                     /locus_tag="VGB88_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX9646809.1"
                     /translation="MARSAAAAGRFPTPSAERALRLLAAAGALIAGCGAAAADPPAEA
                     PVRADGLVSGYWFAAPETRAIQDDDFSNPGMLYVEQGAARWREGDGEAGNSCATCHGD
                     AAGMAGVGATYPKYDAGAGTLINLGQRINLCRRTHMAAAPYEPESGPLLELESFIMRQ
                     SHGLPMNVAVDGPAAPFFARGRDLYLERVGQMDLACTMCHDRRVGHYLRAEHISQGHL
                     AGFPTYMMRWGVMVSRHRRFQFCNEQARAEPLALDSADYNALELYVAWRGNGLAIETP
                     AVRR"
     gene            complement(28488..28880)
                     /gene="soxZ"
                     /locus_tag="VGB88_04885"
     CDS             complement(28488..28880)
                     /gene="soxZ"
                     /locus_tag="VGB88_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX9646810.1"
                     /translation="MALKNHLRRIKVPETARRGEIIAVKTMAEHVMEPGVRMDPATLV
                     VYPRFILNRVECHYNGQLVFWADWFSGVSANPYLTFALRATDSGTITVTWTDDYLGVT
                     SASAEITVLDPPIGEVGGGGREVSDAER"
     gene            complement(28891..29451)
                     /locus_tag="VGB88_04890"
     CDS             complement(28891..29451)
                     /locus_tag="VGB88_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX9646811.1"
                     /translation="MSKTTDHRQRRRGAPSRRAVLAGAGAAGFALAAGPRAFAEDDPA
                     LFSGNPFRDRFQSFRQTFRIEPDREEANRIVAAIIRDRPLRAGLVELTAPDIAEDGNV
                     VPVGFAVACAMAGDDYPAAVHVIAMDNPFPEIAKYHFTPACGEAQVSFRCRMRASSDL
                     VVIAEMADGTVGMATRWVSVTLGACS"
     gene            complement(29569..29982)
                     /gene="soxX"
                     /locus_tag="VGB88_04895"
     CDS             complement(29569..29982)
                     /gene="soxX"
                     /locus_tag="VGB88_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX9646812.1"
                     /translation="MRANDAVVPYRVVGDEIPEPLAGLTGDPARGRAIAAGREGNCLA
                     CHRLPIPEEEFHGDVGPDLGDVGDRLSAGQLRLRLVDPKRVKEDTVMPAFHVVDRLER
                     VAQRYRGRPILGAQQIEDVVAYLKTLGHAGEGGAR"
     gene            30204..>30572
                     /locus_tag="VGB88_04900"
     CDS             30204..>30572
                     /locus_tag="VGB88_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9646813.1"
                     /translation="MPKPGLKGRVGLAVAAACLAAAAAPSLAADEGGRVSQGCAGCHG
                     TDGAAPGATIPILGGQAATYLAESMRAYKRGERDYYVMNIIAQAFDDAQIDAIGAWFE
                     DRPWISTPVPHDAVKASDGKV"
BASE COUNT         4482 a        10893 c        10864 g         4333 t
ORIGIN      
        1 gccgcctggc gctcgagcag ctccgcgaac tcgtacttgg tcgacagccg ctcgccggtc
       61 gcgatcggga tggtggtcgc gcgcgcgacc cgcgccatct cggccatgtt ctcgggcggc
      121 accggctcct cgagccacag cggatcgtac ggctcgagcc gcttggcgag ccggatcgcg
      181 ctcgaggtgg tcatctggcc gtgagtgccg accagcaggt cgcaggcgcc gccgaccgcc
      241 tcgcgcaggt tgcgggtgac cagcgcggcg ttgtcgagcg actcgagcga gagctggcgc
      301 gggtcgaacg ccgacatcgg catcaccggg tcgaacttca ccgcggtgaa gccctgggcg
      361 aggtaggcgg cggcgcgcgc cggcgcgcgc tcggcgtcgc cgaacgcgtt ggcgatgtcg
      421 agcgcgtcct cggctccctc aagctcctcg ggcgagggat acagataggt gtaggagcgg
      481 atgcgctcgt tcaccaggcc gccgatgagg ttgtagaccg gctggtcgag cgccttgccg
      541 atgatgtccc agcacgccat ctcgatgccg ctcaacaccc ccatcagcgc gtggtcgggg
      601 tgctgggtga agccgcgcga gtagacgatc cgccacagcc gctcgatcgc gaacgggtcg
      661 gtgccggcga cgtggcgcgc gaccacgtcc tcgatcatcc gcgccaccag gtgcgggtcg
      721 aacggcacgc cgtaggcctc gccgaagccg tcgatgccgc catcggtggt gagcttgagg
      781 aacacccagc acgggccgcc gcggtgcggc ggcgggttgc ccacgacata ggtcttcagg
      841 tccttgatct gcatcgtccc tcgccgctca atgggggttg tcgggcagtt acgcgcgcgg
      901 ccgcccgacc agggtggcgc cgccgtcgac caccagggtc tggccggtga tgaagcgcgc
      961 acgcgcgctg gccaggaaca cgaccgcatt gcccacgtcc cagccgctgc cctcgacctc
     1021 catcggcgcg gccatgcgcc ggcgctcgcg cgattccgca ctcatccccc cggcgtaaac
     1081 catcggcgtg tacaccgggc cgggcgcgac gcagttgacc cggatgccgt cgccggcgtg
     1141 gtcgaccgcc atcgcccggg tcagcgcgat caccgcgccc ttggacgtcg agtaggcggt
     1201 gagcccgcgc gggcgcagcg ccgagatcga cgagatgttg acgatcgcgc cgccgccggc
     1261 ggcgatcatc gccgggatcg cgtgcttgca ggcgaggaac atggcggtga cgttgacccg
     1321 catcagcctc tcccagcgcg cctcggtctc ctcgaccacg gtcgcctggc cggcgatgcc
     1381 gacattgttg tcgaggatgt cgagccggcc gaagcgctcc agcgcatgcg cgaccatgcg
     1441 cgcgcagtcg tcctctttgg tgacgtcggc ggcgaacgcc tcggcggtgc cgccgaagga
     1501 gtcgatcatc gccaccgtgc gctcggcgag gtcgcgctta aggtcgacca ccagcacgcg
     1561 ggcgccggcg cgggcgaaca ggatcgccgc cgcccggccg ttgccgatgc cctcgccctc
     1621 ggcgccgccg ccggcgacga tggcgacctt gccgtcgagg ccgggatcgt cggggacctc
     1681 gatcatagcg gcgtctcggg cttgaaccgc ggcgcccgct tggcgcggag cgaggcgacg
     1741 ccttcgcgca cgtccggccc ggtgaagccg agcgattcga gcgcgagcga ggcgtcgaac
     1801 gcgggcccgg cggcgcgcag ccagttgttc agcgcgtact tggtccagcg cagcgcggtc
     1861 ggcgcgccgg cggcgaggcg cgaggcgacg tcgagcgcgg tcgcatcgag cgccgcgcgg
     1921 tcgaccgcga gcgagaccag gccgatgcgc tccgcctcct cgcccgacaa cggctcgcac
     1981 agcagcaggt agtacttggc cttggccatg ccgcacaaga gcggccagat gatcgcggcg
     2041 tggtcgccgg cggcgacgcc gagccgggtg tggccgtcga tgatgcgcgc gtcgcgcgcg
     2101 gcgatcgaga tgtcggcgag cagcccggcg gcgagccccg cgccgaccgc cgcgccgtgc
     2161 atcgccgaga cgatcggctt cgagcagttg atgacgttgt agaccaggtc gcgcgcctcc
     2221 ttccagacgc gcacgcgggt gtcgtagtcc tcggcgatgc gctcgatcag ggcgaagtcg
     2281 ccgccggccg agaagccctg gccggcgccc ctgaggatca ccgccgagac ctcgggatcg
     2341 gcatcgatcg cccgccacag ctcggcaagc tgggcgtggg cgcgcgcgtc gaccgacgac
     2401 agccgctcgc cgccgcccat ggtcacgcgc agcacgcgcg ccgcgggccg gtcgaaggtg
     2461 agcgtgtcga acgcgccgta gcgatccgcg ggcgcgtgct cgggaccgtt ctcggtcggc
     2521 atgagtgccc cccctggctg atggtccgag gcaacgctcc gatgcggacg cgcccgtgtc
     2581 aaccgccggc gttcggcgca ggcgacgcta gaccaggtcc ttcggcacca ccagctcgac
     2641 cgcgcgcaca gcctcggggc gaagctcgcg ccagcgcgtc agctcgaggc tgaagcggac
     2701 cagcgcgcag gtcgggaact tgtccgtgat gcgcgccgcg cggccgggat cggcgaggcg
     2761 cgcgagcccc gcggcgaagg cttgcaggcc cggattgtgg ccgaccacca gcacctcgcg
     2821 ccagccgccg ccgacgcgcc gcaggcgggc gccgatcgcc tcggcggggg cgaggtagag
     2881 cgcgcgctcg aagcggatct cggggcgggc gtcgagccgg gcgaggaccg catcgagcgt
     2941 ctcgcgcgcg cgcttggcgg tcgagcacag caccaggtcg ggaaccagcc cgctctgcgc
     3001 gatgtaggtg ccgaccaccg ccgccgcccg ctggccgcgc tcgtccagcg gccgctcgtg
     3061 gtcgcgctca ggcccgagct gccacgacga cttggcatga cgcaggagag cgagatgcct
     3121 gtcggtcacc ggcacgcctc cgtcctccgc gccggcccgg tcaagggcca tgcccggatg
     3181 cgcgcgcggc aggcgcgggc aaagcccccg cctgccgcgg taacgcgccg gtgccgcgct
     3241 gcggcggccg cgacggtgtc agcctacgca gcctcggagt cggcctccag gttgcgcacg
     3301 accctgccga tcagcgcatc caaccgcacc cgttcggccc tggtcatccc gctgagtgca
     3361 atccggtcga cctggcccga caacgatccg agctggccct tgagcgcccg gcccttgggc
     3421 gtgagctgga tgtggatctg gcgccggtcg gcgtcactgc gcttccggtt gaccagcttc
     3481 tcacgctcca tccggtcgag ggtgcggacc atggtcggcg cctcgatgcg gacccggttg
     3541 ctgagctctt tctgggtcag cccgtcctct tcccacaact ggagcaggac cggatattgg
     3601 ccatagccga tgccctttgg tccaagccgc tgcgatagcg accggaccag cagccgcgag
     3661 acaagcgtca tcaggtgggt cgtcatgccc ccgagcttgg ctggagccgc cttccgcgcc
     3721 ggcgatctgg cgcgggccaa aactcgcttg gctggggctc ctttcgcgga agatcgcttg
     3781 gcagacctcc gcttggccgt ggttcgcttg cccgccgacc gcctggccgt gccgcgccgc
     3841 gcggtcgtcc gcttggcggt cgtgcgcttg gcggtcgcgc gtttggccgc ggcccgcttc
     3901 ggcgccgccc gcctcgtcgc ggtgcgcttg gcggtggtgc gcttggtcgc ggcccgcttc
     3961 ggcgccgccc gcttcgcggt cgtccgcttg gtcgcggctc gcttcggcgc ggcgcgcttc
     4021 gcggtcttcc gcttcggcgc cgccttggcg cgcttggcgg tggtcttggc gcccgccttc
     4081 ttgggtgccg cgcgtcgggt ggccttcctc gcggtggtct tggcgcgggc tccggccgaa
     4141 cgagtcgact tcgcccgggt cgttccggcc ttcgctgcct tggtccgagt ggtcctggtc
     4201 ttcgcggtct tggccttcgg ggccgctgcc ttcctcttgg ctgccatgga aatcaattcc
     4261 ccttcgcgat tggatggtat gccgacgccg ccgagcttca ggctctagcg cgccgtgcac
     4321 cttgccgagg cgaccgatat cgcgctcgtc atgaccatgg catatagctc gcaaagcgcg
     4381 aatcgtccac ccctgtttgc gcgccggcgg tcgaatagca cgggcgcggt tagtccgcca
     4441 cccgctgccg ggggttcgat cggccacccg gactgcgagc cgcctaatgc ttggccggtc
     4501 cggaatcgaa accatccggg ctcgcagtcg agtacgaata taggtggggg cagacgatta
     4561 gcgcgctcgg taccggatcc cgcgatcgcg cgcggcgacg cgaaacgtcg aggtcacgcc
     4621 agccagcgct tgcgccggcg gtagtgcttg acgtcgcgat agctcttgcg gccgctctcg
     4681 ctgaggccga gatagaattc cttaacgtcc tcgttctcgc gcagcaccgc ggcgtcgccg
     4741 tcgagaacca cgcggccgtt ctccaggatg tagccgaagt cggcgtagcg cagcgcgatg
     4801 gtcgcgttct gctcggccag caggaagctc acgccctcgt cccgattcag ccggcgcaca
     4861 atctcgaaga tctcctcgac cagttgcggg gcgaggccca tcgacggctc gtcaagcagg
     4921 atcaggcgcg ggcgcgccat cagcgcgcgc ccgatcgcgg tcatctgctg ctcgccgccg
     4981 gatatgtagc cggcaaggcc gctgcggcgt tccttgagcc gcgggaagta gctgtagacg
     5041 aggtcgaggt cgcggccgat gcggtccctg ccgtcgcgcc gcgtataggc gccggtcagc
     5101 aggttctcct ccacggtcag atgctggaag cagcggcgcc cctccatgac ctggatcacg
     5161 ccgcggcgca cgagctcgtt cgcggtcagc cggtcgaccc gctcgccgtc gaagctgatg
     5221 ctgcccttgg tgacctcgcc gcgctctgcc cgcagcaggt tcgagatcgc cttcagggtg
     5281 gtcgacttgc cggcgccgtt ggcgccgagc agggtgacca cgtcgcccgc cggcaggctg
     5341 agcgacaccc ccttgagcac caggatgacg tggtcgtaga cgacctcgat gctgttgacc
     5401 gacagcaggg gcggctcagc gcgattgctg ggcgcccctg ccgtcgccgt catcgcaacc
     5461 gccgggccgg cgcggcggtc agcgcacctc tgcctgcgtc tgctggcact cgcgcaggct
     5521 gatgcccttc tccttggcgt actgagccgc ggagtcctcg accatcgggc ggaccagctc
     5581 ctggtcggtc gggatccagt ccgagatctg gacccatttg ccgtccgtcc actgctggaa
     5641 ctgcaccgcc gcgccgcctt cgtggtcggc gcaactcagc gcgaagggcg ggatcaggcc
     5701 gtcggcgccc agcgccttga tgtgctcctt gtcgagcgcc agccgatcga gcgcctgctg
     5761 gacctccttg ccggtgaccg ggcggtggcc gaccatgtcc atggcgagcc gcagcgcctc
     5821 ggtcgacacg atcgcccaga cgatcgcccg attgtagtag atcgagccga cccgctcgcg
     5881 cggcagctcg ccgttgccct tgtcgtagac gtatttgagg atgtcctgga tcaccgggaa
     5941 gtcggtgccg gagggatgga acccggcggc gatgtagccc tcggcggcat cacccgcggg
     6001 aaccgtgtcc tcctcggcgc cggaccacca cacgccgacc atgtggtcgc gcgggaagcc
     6061 gatgcgctgg gcctccttga gcgcggtcgg gttcatcacc ccccagccgc gcaggatcac
     6121 ccagtcgggc ttgtactggc gtgcgatctg cagccagatc gccgactggt cgaggccggg
     6181 atgggcaacc gggtagtgcc tgacctcgaa gccgtagatc ccggcctgct tgtcgaggat
     6241 cgggatcgtc tccttgccgt aggccgagtc gtgatagacg ttcgcgatct tcttgccctt
     6301 gagctggtcc atgccccctt cccgcatgcc gatgaaccgg atcttggcgg tgttctgcga
     6361 ccagtagttg gtcagcatcg ggaacaggta gggaaatacc cggccgtcgg aggcgtcggt
     6421 gcggccgtag ccgaccgaca gcaccggaat ctcgtccttc caggcgcgct cgaacaccgc
     6481 ataggtgccg ccggtgctca tcgggtggaa gaacggcatg ccggcgccgc ggttcttcat
     6541 ccgctcatag cactcgacga tgcggtcgtt ctgatactgg gtctcgcact tctcccagaa
     6601 cagcttgacc ccgtagatgc cgccgtcgcg ctcattcagc atcttgagat agtcgatcat
     6661 gccgccgccg aaggccgagc cgccggccgc gtagggcccg gtccaatagg cgttgaccgg
     6721 atagaactgc atgtcgctct cgtcctgggc gaaggctgcg gacccgaacg aaagcaccat
     6781 cgccgccgct gccagcgccg atgcgttgct acgaaagccc atgtgttggt tctccctgca
     6841 tggcagaccc atcttcggtt gtggtctgcg cggtctttgc aatgatagca tggccggcgg
     6901 ggcgttcaaa ccaccccagc ccgcctagta ggggaacggc cacagcctga gcttttcctt
     6961 ggcgatcgac cacagcctgg cgagcccggc cggctcgacg atcaggaaca acacgatcag
     7021 gctgccgaac acgatcagct cgaggttcga cagcacctcg gccggcatca ccgagccgag
     7081 ctggccggcg atgttggtga tcagtatcgg cagcatgacg atgaacgccg cgccgaagaa
     7141 cgagcccatg acgctgccga ggccgccgat gatgaccatg aacagcacct ggaaggcgcg
     7201 attgatctcg aacccctggg gctcgaccgt gcccagatag aggaacacgt acatggcgcc
     7261 ggcgacgccg cagtagaacg agctgaccgc gaacgcgagc agcttggtgc gcagcaggcg
     7321 gatgccgatc acctgcgccg ccacgtccat gtcgcgcacc gccatccagg cgcgcccgat
     7381 cgcgctgcgc accaggttct tcatcaccag cgccagcccg atgacgatca gcaccgtgac
     7441 caggtagcgg gattgggcgc tctcgatcgg gaaccccaag agcgtgatcg ccggcgccga
     7501 gatcaccccc gacgccgagt aattgctgaa ccaaccgaaa tgggtgatcg tccattcgat
     7561 gaagaaatgg gcgccgagcg tggtcacggc caggtagaag cctttgatgc gcaggctggg
     7621 caggccgaag aacacgccca ccagcgccgt gatcaggccg gcgacgaaga aggtcgccag
     7681 gaacgggagt gccggaaacg cggtcgcgag cttgtaggcc gagaacgcgc ccaccgccat
     7741 gaagcccgcc gtgccgagcg agatctggcc gcaatagccg agcaggatgt tgagccccat
     7801 cgccgcgacc gacaggatca ggaacggcag cagcaccgag tgcagcaggt actccgaggc
     7861 gagcagcggc accgcgacca cggcggcggc gaggatgccg agcacgaacc acaggtcctg
     7921 ccggatcggg aagatcgcct ggtcggcggc gtagctggtt ttgaactggc ccgcctgacg
     7981 gtagatcacg gcctagaccc tctcgatgat cttctcgccg aacaggccct gcgggcggaa
     8041 caacaggaac acggtcgcca gcacgtaggg gaaccagttc tcgagcgcgc cgccgaaata
     8101 ggggccgatg aacacctcgg cgagcttctc gctggcgccg acgatcaggc cgccgacgat
     8161 ggcgcccgcg atcgagtcga agccgccgat gatcagcacc ggcagcgcct tgagcgcgag
     8221 cagcgacagg ctgaactgca cgccgagctt ggcgccccac agcatgcccg cgatcagcgc
     8281 gatgatcccc gccaccgccc aggtgatcgc ccagatgtgc tgcagcggga tgcccaccga
     8341 catcgccgcc tgatggtcgt cggagaccgc cctgagcgcg cgcccggtgc gggtcttgga
     8401 gaagaacagg ctcaggccca gcaccagcac gccggcgatc ccggcggcga ccagctcgaa
     8461 cacgctcaga tagatgccgg tggcctcgcc gaactcctgc cagggcacgt cgggaatgcc
     8521 gagatcgagg gcgtgcacct ccgcccccca gagcgactgg gcgaagccct cggtgaagaa
     8581 gttgaggccg atggtggcca tgaacaggat gatcagcggc tggttgacga gcggcctgag
     8641 cacgaaccgc tcgaccgcca ccccgtacac gaccatgacc gcgagcgcga tcaccacgca
     8701 gacgccgaaa taccagaggt cgccgctggc gccgaacagg cccatctcga ggaagctgac
     8761 gaaggtcagt gccgcgaaca ggacgaacgc gccctgggcg aagttgaaca cgccggaggc
     8821 cttgtagatc agcgcgaagc cgagcgcgac cagcgagtac atgatccccg acaacagccc
     8881 tctgaccagg acctcgacgg cgaacaacgg atcggagaag gcgacgtcca gcatgtctct
     8941 gtctcccgcc gccgacccgc gcccgcgcgc ctaatggctg acgccgagat aggcgtcgat
     9001 gacgctctgg tcgcggcgca cctcgtccgg cgtgccctcg gcgatcttgc gcccgtagtc
     9061 gagcaccacg acccggtcgg agatgtccat caccacgccc atgtcgtgct cgatcaggac
     9121 gatggtggtg ccgaactcgt cgttgacgtc gaggatgaag cgcgacatgt cctccttctc
     9181 ctcgacgctc atgcccgcca tcggctcgtc cagcagcagc aggcgcggct cggcggcgag
     9241 cgcgcgcccg agctcgaccc gcttctgcag gccgtagggc aggcgcccga ccggggtcct
     9301 gcggatgctc tgaatctcga ggaagtcgat gatccgctcc accgcccgcc ggtgctcgag
     9361 ctcctgcttg cggtacggtc cccagtacag cgcctggagc aggatgttgc cgccggcgcg
     9421 cgtgttgcgc ccggtcatga tgttgtcgag cgtggtcatg cccttgaaca gggcgacgtt
     9481 ctggaacgtg cgggcgatgc cctggcgggc gatctcggcc gggctggcgc cgcgccgctt
     9541 ggcgccgtcg aagatgatcg acccttgctg cgggtgatag acgccgttga tgcagttgag
     9601 cagcgaggtc ttgccggcgc cgttggggcc gatgatcgcc aggatctcgt ggctgcggat
     9661 gtcgaccgag acgccggcga gcgcgaccac cgcgccgaac cggatcgaga tgtcgcgcgc
     9721 gctcagcagc acgtcgccga tctccctgcc gtcggccgac aatcagctcg ccttggccag
     9781 ctgcgccacc ggctcgaggt cgcggatgcc gagcatcgcg gtgatggtgc cctcgcggcc
     9841 gttctcgaac gtcacccggg cctcgacctg cacctcgcgg gcgccgccgt acagcgcctc
     9901 gatcaggtcg gcgtacttct ccccgacata gccgcggcgc accttgcgcg tgcgggtcag
     9961 ctcgttgtcg tcggggtcga gctgcttgtg caggatcagg aagcggcgga tctgcgagcc
    10021 gacgagcttg gggtccgccg ccagcgccgc attcaccttg ccgatctcgc ccgcgatcag
    10081 gtcgtagacc tcggggcgcg cggcgaggtc ggtatagctg gcataggcga ggccccgccg
    10141 ttcggcccag ttgccgaccg actccaggtc gatgttgatg aacgcggtgg cgaagtcgcg
    10201 gccgtggccg tgggccaccg cctccaatat gaaggggctg aacttgagct tgttctcgag
    10261 aaatttcggc gccagcaggg tgccgtcgtt gagcctgccc acgtccttgg cgcggtcgat
    10321 gatgcgcaga tgcccggcgt cgtcgatgta gccggcgtcg ccggacgaga tccagccgtc
    10381 cgcggacttg gtctcggcgg tcgcctgctc gttcttgtag tagccgacga aggtgccggg
    10441 gccgcggaac tggacctcgc cggcctcgct gaccctgagc tcgaccccgg gatacggcgt
    10501 gcccacggtg tcggggctga cctcgccgtc gggctgggcg cagaccagcg cggtcgcctc
    10561 ggtcatgccg tagatctgct tgatgttgat gccgagcgat cggtagaagg cgaagatctc
    10621 cggcccgatc gcctcgcccg cggtataggc gacgcggatg cgcgagaagc cgagattgtc
    10681 cttgagcggc ccgtacacca agagctcgcc gagccagtag aggaggcggt cgccgagcgc
    10741 caccggctcg ccgtcaagca ggcgcgtgcc gacccgctcg gcgagcttta tgaagaagcg
    10801 gaacaggcgc cgcttgatcg ccgccgcgtc ctcgatccgc accatgacgc tggtcaggat
    10861 gctctcccag atgcgcggcg gggcgaagaa gtaggtcggc ccgatctcgc gcaggtcggc
    10921 gagcacggtg gcgccgcttt cgggacagtt gacggtgaag ccggcgcaca gcgcctggct
    10981 gtaggagaac aggtggtcgc cgacccaggc catgggcaga taggcgagca cctcgtcggc
    11041 ctcggtcagc ttgtcatagg cgatgccgtt gtacgacgtg cgctcgaggt tgtcgtaggt
    11101 gagcatcacg cccttcggct tgccggtggt gccggaggtg tagagcatga tcgcgaggtc
    11161 gccgccgcgg cccttggcga cctcgtccat gaacaggccc ggacgctcac ggtcgagccg
    11221 ctcgccctgg cgctgcacgt cctggtacga ggcgagcccg ggctcgttgt agtggcgcat
    11281 gccgcgcggg tcgtgaaaga cgatcagctc gagccgctcc agccggtcgc ggatctgcag
    11341 cagcttgtcg acctgctcct ggtcctcgac cacggcgaag cgcacctcgg cgtggtcgag
    11401 gatgtagtgc atctcggcgg cgatcgagtc ctgatagatc ggcaccggca ccgcgccgag
    11461 cgcctgcgcg gccgcgatcg ccaggtacag atagggccgg ttgtcgccga tgatcgccac
    11521 ccggtcgccg cgctcgaggc cgcgggcggc gaggccgagg gcgaggctgc gggtctcctt
    11581 gagcgcgtcg gcgaagctga gcgcctgcca gatgccgtag tccttctcgc gcaggaacgc
    11641 cttgggcgcg cgcacctcgg cctggtggat caggagcttg ggaaaggtgt cgagcgccgc
    11701 catcgcgccc tggtgaaccg gcacatccat cccactcgcc cctcgcgccg gcccccgacg
    11761 gccggcagcg gatgctgttt cctccacgtt tgcccgacgc cgccgcttgg acgtgccgcc
    11821 ggggcgccga ttatcgggac atcggccgca cgactcaagc cccggcgcca tgaaagggcc
    11881 ccggaaccgg ccggattgct aggtgagcgc gatggcgagc acgtggtcgc cgaccgtgag
    11941 ccgcatcacc gccgcggcgt tggcccggtt catcgccacc tcgaccaggc cgcacgagtt
    12001 ctcgtaccag atcgcctggc cgaggccgac ctcggcgaag gtgcgcgccc gctggacgcg
    12061 cacgtcgccg atcttgagga tcgcgtcgtc ggggagcgtg cttgcccgga tgccggtcat
    12121 ggcgttgccg taatgatcga ggtagatcac ctcgccgagg tcgtcgggcc agcccatgcc
    12181 ggcggcgccc tcggccgcga tcgggcgccc cggcacgtcc tcgccgcgcg ccagcatcgc
    12241 cgccaccggg gcgaacaggt cgcggccgtg gaaggtgctc gacaggcgct cgggccgcca
    12301 ggtgatctgc caccaggcgg catcgccggc gtgcttggcg gcgaccgcga acaggccgtt
    12361 gtcgggaccg acgtaccaga acctgccggc acgcaccacc accgggcggc ggtcggtgcg
    12421 cccgaccccg gggtcgacca cgccgagcac gacgccggcc gcgggcagcc gatcggccag
    12481 cgccgcgagc agataggccg aggccttgtg ctcgaacacc ggcgcatcgt gggcaaggtc
    12541 gatcaccggc acacccggcg ccgccgccgc cagcaccgcc ttgacctggc cgacataagg
    12601 gccgttaccg ccgaaatcgg tgaacagata gatcatcgcc gccatattaa cgcgcattgc
    12661 cggcacgagg tcacgaatgc cacaccgctc tgctcaaccc ggaccgcgca cagcgcaagc
    12721 ggtcgagctc gtggtcgccg cgctcggtgc gcgcggcgac ggcatcgcca cccatgacgg
    12781 ccgcccggtc tacgtcgcgg gcgcgctgcc gggcgagcgg gtgcgtgcgc ggctcggcgc
    12841 ggcgcgcggt gacgggcgcg cggcgatgct cgagcgcgtg ctcgcgccgg ccgcagagcg
    12901 cgtggcccct gcctgccgtc atttcggcac ctgcggcggc tgcgcggtgc agcatctcgc
    12961 gcgcgccgcc gagctcctct ggaagcgcgg cgtgatcgcg gacgcgttgt cgaggcgcgg
    13021 cctcgccgat gcgccggtgg cgccggcgcg cgtggtcgct ccgggcaagc ggcgccgggc
    13081 gacgttcgtc gcggcgcgcg cgggcgcgcg cgtcacgctc ggctttcacc agcgtcgcag
    13141 ccaccgcctg gtcgacatcg ccgagtgccc ggcgatcatg cccgagctcg aggcgctggc
    13201 cgcacccctg cgcgacggcc tcgccggcgt gctccgcgac cgcgagcaaa tcaagcttca
    13261 cgcccaggcg accgagaccg gcatcgacct ggtgatcgac gccgcgcgcg cgccgcaact
    13321 cgccgagcgc gaggcgctgg cggggctcgc cgagcgcctc gacctcgccc gcctgacctg
    13381 gcgcgccgac ggcgagctcg agccgatcgc ctggcgccgg ccggcgcggg tgagcttcgc
    13441 cggcatcgcg gtcgagccgc cgccgggcgc gttcctgcag ccgagtgcgg acggtgaggc
    13501 ggcgatccgc gctgccgtgc tggccgcgat gggccccgcg cgcaccgtcg ccgacctgtt
    13561 cgcgggcgtc ggcacgctcg ccctgccgct cgccgcgcag gcgacggtgg ccgcggtcca
    13621 cgcggtcgac atcgccggcg accatctgga cgcgctcgcc aaggcggcgc gggcgctcga
    13681 ccgggcgcgg atcggcgtcg agcaacgcga cctgttcgcg gcaccgctcg ccgggcccga
    13741 gctcgaccgg ttcgatgcag tggtgttcga cccgccgcgc gccggcgcgc gcgcccaggc
    13801 cgcggcgctc gcggcaagcg cagtgccggc agtggtcgcg gtgagctgcg atccggcgac
    13861 cttcgcccgc gacgcgcggc tcctggtcga cggcggctat cgcctcgagc tcgtcactcc
    13921 gatcgaccag tttccgtggt cggcggaggt ggagctggtg gccgcgtttc gccgcccggg
    13981 ctccggcgcg ggcgcgcgaa ctcgaacgcg gtcgcggcga ccgccgccag cagggtgacc
    14041 agcaggcccg cccacatcca gctcatcatc ggatagatct tgagcaccac cggcacccgc
    14101 gacggcagcc tgagctcggc gagctcgctc cccgccgcct cggcgcggta ctggatcgcg
    14161 ggcagccaca cctggacgtc gcgccacagg ccgcggtgga tgaacgggtg gaccatctgg
    14221 gtcgcgccgc tgaccgtgcg cgcgtagcgg tagtcgacga tctcgcacat caagcgcacc
    14281 gggccgaggc cgccctcggc ctcggggcgc tggtcgcgat agacggcgct gccggtctcg
    14341 ttctgaagcg cgacgccgtc gcgcaccagc gaccagcgca cgtcggcgac cgcgtggaag
    14401 ccctcgccgc cctgggcggc gcgcacgccg tcgcgcacga acgcctcgtg gtcgaccgcg
    14461 accgtcacct cgtagcccca cggaaagcgg atcgcgcgct cgaagtcctc gggataggac
    14521 aggttgcgcc agctgtagag gtcgaacacc gaggcgacga tcgcggccat cagcgccacc
    14581 gctatgcccc catgcaccgc gccgaccggc aggcagtagg ccgaggccgc gcgccgtccc
    14641 gggtggcgca gcaccgcgac cacgccccag gcgagcgcgg cgacgccgaa ataggtcatc
    14701 gccaccgcca gcgcgtcgag ccacgggaag atcgctgcgg tgttggccga ggtcatgccc
    14761 ttgccggtaa agttgctcgc catcggttgc cacagcaacg gtacgagcac gcacgcgact
    14821 gcgaccgcgg cggtcaccgc ccagcaccag gcgcggcggc gcagcggcag gaagttgtgg
    14881 ccgccggcga gcccgacgac gatcccgacc ggggcgagca gggcgttgat ccggtagcgg
    14941 tcgacatccc actggttgaa cagctcgcgc agccgcgccg cctcggccga cgacgtccag
    15001 cgcgccagga actcgaagaa cggcttgaac ttctccggcc gcgcgaggcc gagcgcgtcc
    15061 gatatatagg cctcggcaac caccgcgccc gcagacagcg cgcaggcgag caaggtgatc
    15121 gtggcgatca ggatcaggag cgaggactcg ttccgcgccg gccgacgcgt gcgcaggcgg
    15181 accgaggccg cgaacgcgac cgcggcggac gccgcgagcg cgagtgcgag gaccagcagc
    15241 cacgggaacg aggtctcgcc gacatagcgg tgcgaggagg cgagcgccgg atggcgcgtg
    15301 accaccagcg acagcagcgc cgcgaccgcg gtggcgaggc cgagcgcggg gtggagtcgg
    15361 gcgtaggcgc cctcctggcg gtagcggcgg agcgtatgca ggtgcgcgcc ggccagcgcc
    15421 cacaccgcga acacgctggt ctggaccggg tcccagtgcc agaactggcc gaaggtgaag
    15481 tcctcgtacg cccaccacat gccggacacc agccccgccg acagcaccag ccagccggtg
    15541 cgcacccagc gcgcggcgat cacgccccag tcgccgccgc cggtggccag agcctgcagc
    15601 gcggcgcccg cagtgggcac gaacaggccg taggcggcga gcacgaacgg cggatgcagc
    15661 agcatccaca cccgcaccag atgcgcgttc atgccccgcc cgtattcggt gtccggagtc
    15721 atcacctcgg gcggcagcgc cgcgaacggc gtccacagcc cggcgccgac gccgatcgcg
    15781 gcgacgagaa gaagcgcgcc cgggccggcc cagccgcgat aggcggcgag ccgcaccgcg
    15841 gccagcgagg tcaccgccat caacatcagc agcgtgccct cgtcgcctcc ccacaggttg
    15901 gcgagcttgt agatgtcggg cagcgccgcg ttggagtaca accagacata gcgataggcg
    15961 aactggtcga ggaccagcag cagcgcgagg tcgagcacgg cggcggcgac cagcatcgcg
    16021 gcaaggcgcg ccagcgcgcg ctgtgccgcc ggccgggcaa tgccggcgag cgcgagcgcg
    16081 accgcgcccc acaccgccgc ctgggcggcg agcggcccgg ccgcgaacag cgccggcgcg
    16141 atcggcaccg ccgccggcca ccaggcgcgg ctcaggcggt caagcgtttc gggcatcgtg
    16201 ccgggccttg cgctcggcgc cgtgatagac cgcggccgcg gccaggaccg cgcgcgccgc
    16261 ctctgcccct cgggcgccgg tttccgcgaa ccgcgccgcg accaggccgc tcgcatgggc
    16321 gaccgcgacc gaggcgccgg cgatgcggcc cgcgccgggc gcgccagctt gaacctggac
    16381 acacgccccg aagtcggcct gggcggtggc gaggtgcgag acctcggcga atccgcagcg
    16441 ggcatcgccc gtgacccgga tcaccccggc ataggacgac gggaacaccg gcgcgccgcg
    16501 cgccggcgcc gccgcgatca gcgcgcagcc tgcggcgagc gcgcgtgcgc acgccgcgcg
    16561 cagcggggcg cggtcctcat gcaagccgaa gctcaggttg accaccgccg cgccttgctc
    16621 cgcaagccac gccaccgccg ccgccgcgac cgccgccgag gtggcgccgc gcgcgccgaa
    16681 gatctgggcg ttgacgatgc gcgccgccgg cgcgtgcgcg agcacgatgc gcgccagcgc
    16741 gctgccgtgg ccgaccgggt cgggcatcgg cgcggcggtc tgcgcctgct cgccttcgcc
    16801 cgccgtgaag ccgcgcgccg cgaccacgcg cgcgagaagc tcgtcgtcga cgccgctgtc
    16861 ggcgagcccg accagcacct gcctgcgcga agccgaagcg gcttcgcttc ggcgaaggcg
    16921 ggcgggctca gccatggccc ggcgccggcg cgcgcgccgg gcgcgcgtcg cggaaccgtc
    16981 cgcccgccgc ctcgaccacc cggtcggcgc cggccagggt ctcggcgcgg tggctgatga
    17041 cgatgcgcgt gcgcccggcg aacagcgcgt cgaccgcggc gatcagccgt gcctcggtgg
    17101 caaggtcgac cgccgaggtc gcctcgtcga gcaccagcac cagcgggtcc tggagcagcg
    17161 cgcgcgcgat cgcgagccgc tggcgctggc cgcccgacag ccgcgcgccg cgctcgccga
    17221 tcatggtgtc ggcgccggcg ggcagctcgc cgacgaactg gtcgagctgg gcgcggcgga
    17281 gcgccgtgcg cacctcgtcc tcggtcgccg caggtgccgc atagcggacg ttgtcggcga
    17341 tggtgccgcg gaacagcacc acgtcctggg cgaccacggc gatgcgccgg cgcagctcgg
    17401 cgaggtcgag agcgcgcagg tcgatcccgt cgagggtgat gcggccaagg tcggggtcga
    17461 aatggcgctg gagcaggtcg atcaaggtgg tcttgccagc gccggacggg ccgagcacgc
    17521 cgagcttggt gccggcggga atgtcgaggc tggcgtgctc gagcaccgca tgatcggtgc
    17581 ccggataggc gaacgacaca gagtcgatgc ggatcgcgcc ggcggcggtc gcgggcagcg
    17641 ccgcgggcga aggcggcgag accaccagcg gcgcggtgtc ggtgagcgcg cgcacgcgcg
    17701 cgagcgagac ccgcgcgcgc atcatgccga catagatgcc gagcaggctg tgcaccggcc
    17761 cggtggcgaa gccgagatag gtgttgaacg cgatcagggt gccgagggtg agatcgccgg
    17821 cgacgatcag gtagccgccg gcgaggaaca cgccggcgcg gctcgccgag gtcagcaggt
    17881 tgggcaccgc cgcggtcagg aactcggcca actgcaggcg caagaggtcg ccgagatagc
    17941 gccggttcag cccttcgagc cgcgccgcct cgcgcgcctc ggcgccgacc gactgcacga
    18001 acttggtcgc cggcaaggtc tcgaccagga atgccgacag gtcgctggcg cgcgcgcgcg
    18061 tcgcccgggc gcgcgactcg acccgcgggc gcatcacgcg cagataggca tactccgccg
    18121 gcagcagcac caaggcgacc agcgacagct gccagctcag ccacagcatc agcgccaagg
    18181 ccgccgcgag cgcgaaggcg ccgttgatgc cggcgagcag cgagtcgacg cagaagcgct
    18241 ggacctgggc gacgtcgccg tcgaggcgct ggagcacgtc gccggtgcgc agccgcgcga
    18301 agaacgcggg cgagagcgtc tgcaggtggc ggaacaccgc ctcgcgcatg gcgaacagga
    18361 tgcggccaga gaccttgacg tgcagatagc ggttcagccc gccgaagccg agattgccga
    18421 gccccaggcc gagcatcagc gcggtcagcc aggcgaggcg ggcgaggtcg ccggcgagca
    18481 cgccgtcgtc gatcatcagc ttcatgatgt agggctgggc ggaggtcacc gcggtggtcg
    18541 cgaccgcgag cgcgagcacc agcgcaagcc cgcgcgcatg cgggcggagg aacgacaccg
    18601 cccaggcgag cgtgcgccgg tcgtccgccg ccgtggtcgc gtccgcgccg atgtcctcgc
    18661 tcacccgtct cgcacccgcc aaccggaccg tcgagcctag cctagctgcc tgccatggcg
    18721 gcaaaaaaag gggccggcaa cgcgccggcc ccgcatcctg tcgagcccgg aggggcggct
    18781 agcgctggat cacgcggatg ccggccacgc cctggtcgcc gccgggaagc atcaccttcg
    18841 ccagctgctt gagcgtgtcg gtggagtaga tggcgacgtc gttgaacgtg ccgccgatat
    18901 agagctcgtc gccctcggcc gagattgccg ccgcgtagta ggtgtggtcg aggtcgaccc
    18961 ggtcgaccag cttgccggtg tcgaggtcgt acttcgacag cgtggtgtag accgcgtaga
    19021 tctcgctcgg cttcttcgga tgggtgacca tcgagaagat cagcacgtcg aacgaggtga
    19081 tgtcgcgttg cgcccactcg ttggtgcgca ggtccacggt ggtgatgccg agcacgaagt
    19141 ccgagagctg gtcgaagtcg cccgcgtcgc gcgcctcgcg gctggcgaac accggcgcga
    19201 cgtaagggaa gatcatgacg tcgatgttgt cgtagagcgg ccagaacgac agcccgtcgg
    19261 ggtcgccgta ggtcggcttg tcctcctccc agctattgag ccggatctcc tgcaggatct
    19321 tgccggtggc gatgtcgaac tggcgcacct cgtggccgta gctccacagc gtcttgccgt
    19381 cgtgcgaggg cgccatgatc gtctgcccgc gcggcacgtc gaacgagcgc gccggcttgg
    19441 cgtcgagccc ggcatcggtg ttgtagacca ggaaccggtg catcggccgg atctggtagc
    19501 ggtcggcgag cagctcggtc tcggcgacct ggacgaacag ctccttgccg tcgaggccga
    19561 ccgcgacccc ggccatgctc cgcgcgcgca ccgtgccctg gctgagcacg gcgtggaaga
    19621 acacctcgca cgagtcgatg ttgacgccga ccacctcgcc ccagccgttg gtctcgacga
    19681 acgccaccgc ggggtcgacc ggcgaggtgg cgatcgccac cggcgagccg cgcccgggca
    19741 gggtgcagct gttgaccacc ttgcgcgcgt cggcgtcgat gacgtgcagg ttgttcggct
    19801 tgttgacggc gatgacgtac tccttggcga gcgcgccggt ggcggcgagc gccgccaggc
    19861 ccgcgcccgc cagcagcgcg atgctggcgg ccctgagcag cctgttcatt gtcgtgccct
    19921 ccctttcacg ctgcccgagc gacggctcag acggtgccgt cctcgctcgg gaacaccgac
    19981 tcgagcaccc gccagtcctt ggcgctcggc gcgttggcgt tccagtcggg ataggtgttg
    20041 atggtgtccg gaacctgcgc cggccaccag cacgggtccg agcagccgta gagatccgcc
    20101 tccatcggct ggcacaggcc ggccacgccg ccgaacgggt cgacctccca gccggggtcg
    20161 agggtcgagg agcagccggc gaccacggtc tgcatcatcc gaacctcgtc cgccttgccc
    20221 tgctcgagtg ccgactcgag cagcttcgcc ttgcggttga acgacttgag gtgcttcatt
    20281 tgtaggccct ccgcggctcg acataggttg cgaagaacgc cgggttgcgt tccatgatcc
    20341 gggtgtagac cccgatgccg aagtcgaccc agtcgcgcat caggtcgcag tagtgatagg
    20401 tcgggtggat cgggtcgttg tagcgggcgt aggactcgtg atagcagccg cccgagcaca
    20461 ggttgcggat gcggcaggtg ttgcaggcgg tctcggagcg gtcgaggcgc gattcgagga
    20521 agcgcccgag cgccggcttg tcgatgccgt gatcgacgtc gccgaaggtg tccaggcccg
    20581 agccggtgaa gcggtggcac aggttgagcc cgccgccggt gtcgaccgcc agcagcccga
    20641 gcccggcgcc acagggcagc gccttgctca tgccctcgga cagatccgtc atcagctggt
    20701 gcatgttgct gaagccgatg ttgcgatgcg cgaacgccgc ctccaggtag atgccgccca
    20761 aggtcttcat gttggcgaac agctcggcaa gctcgtcgcc gttgagcgcg aatatggcgt
    20821 gctcgcccga cgtcaccggc gcgaagccga cctcgtgaaa gccgatgtcg ttgaccaggt
    20881 ggtcccagat cgcgaccacg tcgaccacgc ccgcggtcaa ggtgacgcgc gcgccgatcg
    20941 ggcgcgagtc gtatctctcg agcgccatcc gcgccttggc cgcgaccacg tcgtaggagc
    21001 ccttgccgcc gaccgtgcgc cggcgcaggt cgtggaccgc gcgcgggccg tccatgctga
    21061 tcgtcagccc gaagcgatgg gcgttgaagt agtcgaccaa cctttcggtc agcaacgtgc
    21121 cgttggtggt aagcgtgaag tcgaccttct tgccgagctc gctcgcgcgc gcctccgcgt
    21181 aatcgaccac ggcgcggatc agcgccatgt tgctgagcgg ctcgccgccg aagaacacga
    21241 tgttgaagcg ctcgcgctgg gcaccctctg cgagcagcag ctcgatgctc ttgcgcgcgg
    21301 tctcgaactc catcttggtg ccgttggccg gggtctcgag gtcctccttg tagcaatagg
    21361 tgcagctcag gttgcagccg gtattggtgt tcagcacgat ggtcgacagc gggaagttct
    21421 cgatccggat cggcgcccgc tccggcgccc gcgcccgccc gtccgacagc acctcgagct
    21481 cgatgaagtc gcggatcgcc tcgaccacgg cctcgggcgc gtggcggccg tcgaagctct
    21541 cgcgcaccag ctcgggcgtg accgccgggc gcgcgcccga cgattccgca tcgaacaggt
    21601 cgagcaggtc gcccgagacc gcatcgagct cgaacaggct cgaggtcggg atgtggaaca
    21661 tcatccgccg gcccgcgacg cggacctggt gcagattgtg ctcgaccagc ctcagctcgc
    21721 tcatcgcgcc tccgcgcgcc gccgcccgcg acccgctccc tagcggatcg gcgggtcgtt
    21781 ccagcgctgt accgtgacca gcagctggcc ttcgcccgcc accgcgttgc cggcgtcgtc
    21841 gaccgtgccg atcactttca ggttgccggc gttgttggtc tcgaacgggc gcgccgggtt
    21901 gagcccggcg tcggccggat cgaagatgcc ggtcttcggg tccatctggc cggcgaagcc
    21961 gacgtcgtcg agctcggccg cgacctcgtc gaacggctcg accgaccagg tcgccggcat
    22021 cacgccgatg cggacatcgt cgtcggtgcc ggcggcgccg tcgggcccgg ccgtgaaccc
    22081 gaccgcgtcg aacagcgcgt attggtcggg gcgggtgccg ccgccctcgc cgacgcgggc
    22141 gaaggcgtag gcgggctcga ccgcgacccg cgcgaccgcg tcgtagacca ccaacgcatc
    22201 gttggccgag gcggcatcgc cgaccttgat cgcgcgcgcc ccgagcgcgg cgtcggccgc
    22261 ggccttggca ttaaccacca ccatcgtcga gcccgaggat acggtgttga ccacctcgac
    22321 gcccgcgccg agatcgaccg cgccgttgag gccggtgccg tggatcgcga tctcggcctc
    22381 gctgccggcg cggatatggg cgggctcgac cgccatgatc tcggagtggc cgtcgcgcat
    22441 gcgcacagcg acgaagtgcg cgccggtctc gtcgtgcgcg ctcagcatga agcggccctc
    22501 cgcgcgattg ccgtcctccg acagggcggc gatcgacagg tactcgtcgc cgctggtcgc
    22561 ctcggtcagg cgggcacgga gctcgtaccc ggtatagatg atcgccgaac cgctggcctc
    22621 gaccgccgac ccgtccaggt tgaactcgct gtccgaggcc gaataggtgt cctcgccggt
    22681 tgcgctgaac gtggtgatgc cgttgtggaa gccgaccccg gggccgcgcc cgacccagcg
    22741 ccagtcgccg gacatttcga ccgaatggcc cttccagtcc tgccaagcct ggctgtcgaa
    22801 cgggtagtgc tcgtacagcc agtcgacggt ctcggtcgag gcgatccggt accactcgcg
    22861 gtcgcggccg tagaactggt actcggtggt cggccattgg ccgaggtgga agtcgatgtg
    22921 ccagcgccac tcgtccttgg tccggcgctg gagcgcgccg cgggcgcccg agtggcagcg
    22981 cgcgcacatg tagaagtatt cgggcgggaa gtcctcgatt tgcgccggct cgcgctcgag
    23041 gatgtagcgg tagccggtgg tctccgacgg cgccaggcct tggctgtcgg ccaggtactt
    23101 gaccagcgca cgccgctcgt ccgcgggcac ctcgacgccg tgcatgatgc ccatgcgcac
    23161 gatcgtcatc agccagccct cgggcgtctt gcgctggccc ttgatgcggc tgagcccgtc
    23221 gccctcgtcg acgtggcagg cgccgcagcg tgcattcagg atcgcctcgc cgtccgcggc
    23281 ccgcaccccg tccgtggata gcaccaacgc gatcagcgcc accgcgccga ccaaccagcc
    23341 gaaagcccaa tcgcgcaccg acatggcagc ggtccctccc cttggcccga caccgcaccc
    23401 ggcgaccgca gcatccccgc gacaggacgc ggctcccacc acgaccgcgt atgtatgggt
    23461 gatgtttgac cctgggcgga accgagtcaa gcgcgcttca ggccgaggcg ccgccgcgcg
    23521 ggccggcgtc cgcaaccggg tgcatcgggc gcgcggcgag caacccgcgc cgcgcgagcc
    23581 aggcgagcgc ggtttcgacc cgcgcccttg ggcagcgaag atgcgccgcc gccgccgccg
    23641 ccaggccgcc gccgtcctgc cagccgccgg ccaggaagcg gatcagcggc accagcgcga
    23701 ccccgtccac ctgccagacg ccgcgcgcgt ggtcggcggt gaccaccacc tcgcgctcgc
    23761 tgacgtagcc gccgtcgatc accggcaggc gcgcgatgcg ggcgggcgcc gcgtccgggc
    23821 gcgggtgcgc cggcgcatcg tcgggccacg gcgtgcgcgc cgcccagaac gggcgggcgg
    23881 cccagcggcg ctcgagccgg tagaagtcgc gcccgacacg cgcctggacc aggaacgtct
    23941 cctcgatgcg cgcgccgtag aaccgttcgg cgagcgcgcg gtcgcccgcc cggtcgagga
    24001 gggtgttggc caccgccacc gcattcagcg cggtcgaggc ggcggtgaac tggccatggc
    24061 ccgacagcgg gtcgatcgcc accgcggcgt cgccgatgcg gatcagaccg gggccgcagg
    24121 ccggcgcgca cagcacggcg tcggcattgc gtgcgaccaa cggccccgca ggcccggcgc
    24181 cggcaataag ctcggcgacc tcgggcacgc cggcgatccg ctcgctgaag aacgcctcca
    24241 gcgcgcgccg cggcggcagc gcctcgccgg cgaccaccac ctgcaccgcc gcggtgccgt
    24301 cgccgggggc ggccagccac agccagccgt cggcgaagct cgcgaccgtg ctttgcggcg
    24361 cgattgcgcg cgcgagcctg agccggcgca ccagcgcggt cgccggcggg ccgtggcggc
    24421 ggcgcgccgt cccggccggc gcctggcgcc cgcgcgcctc gatcagcagc gccgcctcga
    24481 cctcgacgcc accgcgcgcg agggcccgcc agccgccctt gatcgggagc agcgcggcaa
    24541 tgcgcgccac cgtcacggcg gcgccggcgg cgcgggcatc gtcgagcagc gcggcatcga
    24601 ggcgggcgcg gtcgaccaag gtctcgccgt tggcctgcac gcgccggccg ttccaatggc
    24661 tgaagcgctc gacccacggc ccggcaacat ccagcgcccg ggtgcagccg gcgaagcgaa
    24721 agccgtccgc ggcccgctcc gaccggcctt cgatcagcga ctggcggcgg cggctggtga
    24781 tcaggtggac acggcgcccg gcacgcgcca gcgccgcggc ggcgaccgcc ccggcgacgc
    24841 cgccgccgac gatcaccacc tcggcgcgtg ccatcgccgc cgaccgggcg cgagcggcgc
    24901 ggtcaggcgc tgatgtattc gcgcagctgg tccgcctcgc gctcggcgct ggcgatgcgc
    24961 tgcttgacca cgtcgccgat gctgaccagg ccggcgagcc ggccgtcttc gagcaccggc
    25021 aggtggcgaa tacggcgctc ggtcatctgg ttcatcacat cgacgatcgt ggtgtccggg
    25081 gtacagctga tgacgtcggc ggtcatcagg tcctcgacct tgcggtcgag gcacgccgac
    25141 ccctcatcgg cgacaccgta gacgatgtcg cgctccgaga tcacgccgat cagcgcgccc
    25201 tcgccgtcga ccaccaccgc cgcgccgatc cggcgctcga ccagctcctt ggccagctcg
    25261 gcgatgctct gatcgcgcgt gatcgtgaac acctgcgtgc ccttagtctt gaggatctct
    25321 ctcacgagca tgtcgcgcct ccgtgtcgga cggccacgcg gcacgggcaa cgggccacgc
    25381 tgcaagacgc gcgcggcccc ccgaccgctg tcgctccggg ggccatgata gcagaaaacg
    25441 gcgcccgcgg cacgccggct gagcgcaggc gagcggctac tccgctgccg ccgcctgggc
    25501 gacgacgtcg tcggccagct tgcccgacag ctcctgcatg tggtcgaact ggtagtcgta
    25561 cgagcccacg ccgcgggtca gcgagcgcag ctcgatgatc aggtcgttca tctcggcctg
    25621 ggcgagctgc gccgagacct ggtcccagcc ctgccagccg ggacgggtct cgaagccgag
    25681 gatctggccg cgccgcgtgc tgagcagcct ttgcacctgg gcggtgaact cgttgggcac
    25741 gaacacggtg acccggtaga tcggctcgag caggaccggg ttgcactgcg gcagcgccgc
    25801 ctgcatggcg atccggccgg cggtctggaa ggccatttcc gagctgtcga ccgtgtggta
    25861 ggagccgaac accagcttga ccgagaggtc gaccaccggg aagccgagcg gccccttctt
    25921 gcagaagtcg accaccccgg tctcgaccgc cgggatgtac tgcttgggca ccaccccgcc
    25981 ggtgatcgag tccttgaact cgaagccgct gccgcgcggg cgcggctcga ccgtgatgta
    26041 gacgtcgccg aactggccgt gaccgccggt ctgcttcttg tgccggccct ggacgcgctc
    26101 ggcgcgcttg cggatggtct ccttgtaggg cacccgcacc ggctcggtga ccagcggcag
    26161 gttgaacttg ttgcgcagcc ggtcgagcgc gaccttgaga tgcatctcgc cctggccgcg
    26221 cagcaggtgc tggtgggtgt cggggtcgtg ctcggcgacc agcgagcggt cttcctcgag
    26281 cagcttgccg agcgcgtccg acagcttgac ctcgtcggcg cgcttctcgg cggcgatgcc
    26341 gaaggcatag accggcttga gcggcgcgac caggagggcg tcgggaacct tggcggcgcc
    26401 cgcgctcgac agcatgtcgc cggtcccgac ctcgtccatc cgcccgagcg ccacgacctc
    26461 gccggcaacc gccttcgcca ccttggtcgt ggcctggccc aacagcttgt tgacgctgcc
    26521 gacgcgctgg ccgttcagcg tcatgccgtc ggtaagcgtg ccgcgccaga cgcggaccag
    26581 cgacagcttg ccgctggtcg cggtgttgta agtcttgagc acttgggcga gggtgccctc
    26641 gccgccggcg acgccgacgc gctcggcggt cgactgggcc tcgggcaggt cgtggcgcag
    26701 cgccttgagg aggcggcgca ccccgtgctc gtgctcgccg gcgccgagca ggaccggcgc
    26761 caccagcccg gcgccgaagt ccttgctcat cgcctggaag atctcctcat tcggcgggat
    26821 cacgtcctcg agcagctgct cgagcagggt gtcgtcgaag tcggcgagcg actccaagag
    26881 ctcggtgcgc gcggccttct cgtccgccgc catcggctca gggatccgga tcagggccga
    26941 ggcggcgtcg gcctggtagc ggaacgcgcg ctcgtggacc aggtcgatgt agccggtcag
    27001 ggtctcgccg tcgcggatcg gcacctggcg cagcaccagc ggccggcccg cctgcgcctg
    27061 catcgccgcc agcagatcct tcaccggcgc ggcggcgcga tccatcttgc tgatgaacac
    27121 catgtagggg atggcgcgcg catcgaggaa gttgagcacc ggcgccagcg ccaccaggcg
    27181 gtcgatctcg ggctcgacga ccacgatcgc ggcatcgacg ccgaccagcg catcgagagc
    27241 ctcctggagg aactccagcg accccggaca gtcgatgacc gagaacgact cgcccatgaa
    27301 ctcgaagctc gccaccgata cctcgacgct catctggcgg gcgcgcgcct cggtggagcc
    27361 gtcgccgacc atgttgccgt ctttcaccgt gcccttgcgc gagatcgcac cggccgcgaa
    27421 cagcaggttc tcgagcagtg atgtcttgcc gccggaaaac ggcccgacca gcgccacgct
    27481 gcgcggtgcg gaaggtgcct tcgtggtcat gttcagctgt cggccggagc tgccccggcc
    27541 gctcccgttg tggcgggcgc gcgcgtctcg gccaccgcgc gcccgggcga ttcctctctg
    27601 accgccccat tgattccagc gacggcgccc tgccgcaaca aaaagatcgg caaccgggcc
    27661 tcggcgccgc gccggcgggc aacgagtcgc ccggctatcg cctgaccgcc ggtgtctcga
    27721 tcgccagccc gttgccgcgc caggcgacgt agagctcgag cgcgttgtag tccgcggagt
    27781 cgagggcgag cggctcggcg cgcgcctgct cgttgcagaa ctggaaccgc cggtggcgcg
    27841 agaccatcac gccccagcgc atcatgtagg tgggaaagcc ggcgaggtgg ccctggctga
    27901 tgtgctcggc gcgcaggtaa tgcccgaccc ggcggtcgtg gcacatggtg caggcgaggt
    27961 ccatctgccc cacccgctca agatagaggt cgcgcccgcg cgcgaagaac ggcgccgccg
    28021 ggccgtccac cgcgacgttc atgggcaggc cgtgcgactg gcgcatgatg aaggactcga
    28081 gttccagcaa cggcccgctt tcgggctcgt agggcgccgc cgccatgtgc gtcctgcggc
    28141 acaggttgat gcgctggccg aggttgatca gggtgccggc gccggcgtcg tatttgggat
    28201 aggtggcgcc gacgccggcc atgccggcgg cgtcgccgtg gcaggtggcg cagctgttgc
    28261 cggcctcgcc gtccccctcg cgccagcgcg ccgcgccctg ctcgacatac agcatgcccg
    28321 ggttggagaa gtcgtcgtcc tggatggcgc gcgtctcggg cgccgcgaac cagtagcccg
    28381 agaccagccc gtcggcgcgc accggcgcct cggccggcgg atcggccgcc gcggcgccac
    28441 agccggcgat gagcgccccg gcggcggcga gcaggcgcag cgctcgctca gcgctcggcg
    28501 tcggaaacct cccggccgcc gccgccgacc tcgccaatcg gcggatcgag gaccgtgatc
    28561 tcggcgctgg cgctggtgac gccgaggtag tcgtcggtcc aggtgacggt gatcgtgccc
    28621 gagtcggtcg cgcgcagggc gaaggtcagg taggggttgg cggagacgcc cgagaaccag
    28681 tcggcccaga acaccagctg gccgttgtag tggcactcga cccggttgag gatgaagcgc
    28741 gggtagacga ccagggtcgc cgggtccatg cgcacgcccg gctccatgac gtgctcggcc
    28801 atggtcttga ccgcgatgat ctcgccgcgc ctggcggtct cgggcacctt gatccggcgc
    28861 aggtggttct tgagcgccat cgcccgcccc ctagctgcac gcgcccaagg tgacgctgac
    28921 ccagcgcgtg gccatgccga cggtgccgtc ggccatctcc gcgatcacca ccagatcgga
    28981 gctcgcgcgc atccggcagc ggaacgagac ctgggcctcg ccgcacgccg gggtgaagtg
    29041 gtacttggcg atctccggga acgggttgtc catggcgatg acgtgcaccg ccgcgggata
    29101 atcgtcgccg gccatcgcgc aggcgaccgc aaagccgacc ggcacgacgt tgccgtcctc
    29161 ggcgatgtcg ggggcggtca gctcgaccag gcccgcgcgc agcggccggt ccctgatgat
    29221 cgcggcgacg atgcggttcg cctcctcccg gtcgggctcg atccggaagg tctggcggaa
    29281 gctctggaag cggtcgcgga acgggttgcc cgagaacagg gcggggtcgt cctcggcgaa
    29341 cgcgcgcggc ccggccgcca aggcgaagcc ggcggcgccg gcaccggcga gcacggcgcg
    29401 ccggctgggc gcgccgcggc ggcgttggcg atggtcggtg gtcttgctca ctctctcggc
    29461 ctctgcgcga cgtccagcaa accagacccc gtgcgcggcg cgaaatcaac tcgccgtgcg
    29521 caggcaacca gcaagtgaat gtagcatttc gcgcgcccgt ccgcccgctc agcgcgcgcc
    29581 gccctcgccg gcgtggccga gcgtcttgag ataggcgacc acgtcctcga tctgctgggc
    29641 gcccaggatc ggccggccgc ggtagcgttg cgccacccgc tcaagccggt cgacgacgtg
    29701 gaacgccggc atcacggtgt cctccttgac ccgcttgggg tcgaccagcc tgagcctgag
    29761 ctggcccgcg ctcaggcggt cgccgacatc gccgagatcg gggccgacgt cgccgtggaa
    29821 ctcctcctcg gggatcggca ggcggtggca ggcaaggcag ttgccctcgc gcccggcggc
    29881 tatcgcgcgc ccgcgcgccg gatcgcccgt gagcccggcg agcggctcgg ggatctcgtc
    29941 gccgacaacc cgatacggga ccacggcgtc gttcgcgcgc acgccgccgg gcgccgccgc
    30001 gagcacggca cccagggcga tagccgcgca cgtccggtgg ccgccaggac gggatcgatc
    30061 catggggcgc gagtctgcgg cgcgcgcgcg cgcctgtaaa cggtacccgc gcagtcaata
    30121 aatcgcgatc gcgcctgttc ccggcgccgc cgcctcgact atcatgtcgg ggcacgcgcc
    30181 agaacgtgcg caggggagga gagatgccga aaccgggact gaagggccgg gtagggctgg
    30241 cggtcgccgc cgcctgcctg gccgccgcgg cggccccgtc gttggccgcc gacgagggcg
    30301 ggcgcgtgtc gcagggctgc gccggctgcc acggcaccga cggcgccgcg ccgggcgcga
    30361 cgatcccgat cctgggcgga caggccgcga cctatctggc cgagtcgatg cgcgcctata
    30421 agcgcggcga gcgcgactac tacgtcatga acatcatcgc ccaggcgttc gacgacgccc
    30481 agatcgacgc catcggcgcc tggttcgagg accggccgtg gatcagcacc ccggtgcccc
    30541 acgacgcggt caaggcgagc gacggcaagg tc
//
LOCUS       DASWHU010000134         5348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1539719, whole genome shotgun sequence.
ACCESSION   DASWHU010000134 DASWHU010000000
VERSION     DASWHU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539719"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGB88_04905"
     CDS             complement(<1..776)
                     /locus_tag="VGB88_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9646814.1"
                     /translation="MATSRPAIKTTVVGSYPVPAWLAAHPTRQALIDATRVVLHTQEQ
                     AGIDLVCDGELSRFDVNHPETNGMIDYFVRPLAGVRTEITFDEARAWRAKAGMAYRRR
                     PPGVVEGALASGALDLPAACAEARRLTKAPFKFTVTGPHMLAKTLLDAHYGDIEALAD
                     ALAGVLAEQVAQCEADVIQVDEANLPGHPEEWPWAARAINRVLAAVRGTPAVHLCFGN
                     YGGQVIQRGSWDKLIAYLNSLQVDHIVMEAAHRPAGELAA"
     gene            913..1914
                     /locus_tag="VGB88_04910"
     CDS             913..1914
                     /locus_tag="VGB88_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9646815.1"
                     /translation="MPRLLAMLAFALLLGGAARAGDYPWQPTRPITIVVPWSAGGSTD
                     QVTRVLAGELELALGQKVVVINQPGASGSIGKRNVWQAPHDGYTWAAGAPKMLGTYKL
                     VGLFDTRIEDWRLYLDAVTPTLIGVNPDTPYQDMGDLLEAMRARPGEISVATAGVGSS
                     GHTTMEAIAQATGIKYRNVSYDGGNPAVIAAVSGECEVTTQLSTEQIEMARAGRLRIL
                     AVVADHAVTLEGLGTIPPVTDWTGPIPNTLTHFGIFAPADLPAEVIETFDKVWETRIK
                     TSKALRDYAAERGTVFETAFGAAAQTAVFPTVQADAWTLYGAGRTTMSPDELGIPKP"
     gene            1940..2440
                     /locus_tag="VGB88_04915"
     CDS             1940..2440
                     /locus_tag="VGB88_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9646816.1"
                     /translation="MVKADFVTGLAFLALGIAALIESLRMPRFETLAINPYTVPGIVP
                     GLLGVVIAALGAMLALRAARSGGWRVAPALARLATLHRSEGAQRLALALALTFGYAAG
                     LLGRLPFWLASALFVFAFIALFEWRAHIEAKARWRALAFAAGEAAVASAAVTLVFRYL
                     FLVNLP"
     gene            2437..3942
                     /locus_tag="VGB88_04920"
     CDS             2437..3942
                     /locus_tag="VGB88_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9646817.1"
                     /translation="MTGGLADLVAALVGFLAPEPLFSVLWATLLGIAVGMLPGLTATL
                     GVALMTTLTFRMDAGQAILILICMYVGAIYGGSRSAILLNIPGTPASAATTLDGFPLA
                     RAGRAGGAMATATSGSVLGTLIGMVFLAALAPVLAEFALDFGAYEFFWLALFGVVISG
                     HLTALGDPLKGWLAGFLGLLLAMVGQEGIHAYERFSFGSTDLAGGLGLLPALVGAFGF
                     AEILIVMKSPAYEVVRGKVDSVIPRIGEVLRYRWTILRSGVIGTGMGLIPGVGEDMGA
                     WVSYAAARRLSRERAQFGKGSIEGLMAAETGNSAAVPGAIIPVLTLAIPGSAPSAVLL
                     AAMFIHGVRPGPLIMIEFPGFVHQVVAMVFLASLAILLWGIVLTRPLLTVLRVPRERM
                     MPVVFVLCTIGSFAIAGRLFDVWVMLGFGVLGFALRQMDYPMAPLVLGIVLGDILDKS
                     LRRGLVLSDGDPSPFFARPISAVLAAVTLATIAFSIPVVRRAVGRVLGGAT"
     gene            3939..4781
                     /locus_tag="VGB88_04925"
     CDS             3939..4781
                     /locus_tag="VGB88_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX9646818.1"
                     /translation="MNIALCNEVIRELEFPAQCGFAAALGYDAIELAPFTLGPEPHLM
                     GAGERARLRRAAADAGIAISSLHWLLVVPKGLSIHTPDDALRARTIEVMRRLIGLCAD
                     LGGTAMVHGSPMARRIPDGADAAEYRARALDTFAAVAADAEAAGVAYCIEPLAARETN
                     FVNTVAEAAAMVEAIGSPALRTMIDTCAAAQAEALPVAELIDRWLPTGLIGHIQVNDR
                     DQSGPGQGADSFAPAFAALRRNHYRGTVAVEPFVYFPDGPAAAARAIGYIRGILEALD
                     WRDQ"
BASE COUNT          689 a         1915 c         1893 g          851 t
ORIGIN      
        1 aacgcggcga gctcgcccgc cgggcggtgc gcggcctcca tcacgatgtg gtcgacctgg
       61 aggctgttca ggtacgcgat cagcttgtcc cacgagccgc gctggatcac ctggccgccg
      121 tagttgccga agcacaggtg gaccgccggc gtgccgcgga ccgcggcgag cacgcggttg
      181 atcgcccgcg ccgcccacgg ccactcctcg ggatgtcccg gcaggttggc ctcgtcgacc
      241 tggatcacgt cggcctcgca ctgggcgacc tgctcggcga gcacgccggc gagcgcatcg
      301 gcgagcgcct cgatgtcgcc gtaatgcgca tcgagaagcg tcttggccag catgtgcggg
      361 ccggtgacag tgaacttgaa cggcgccttc gtcagccggc gcgcttccgc gcacgccgcc
      421 ggcaggtcga gcgcgccgct ggcgagcgcg ccctcgacca ccccgggcgg gcgccgccgg
      481 taggccatgc ccgccttggc gcgccaggcg cgcgcctcgt caaaggtgat ctcggtacgc
      541 accccggcca gcggccggac gaagtagtcg atcatgccgt tggtctcggg gtggttgacg
      601 tcgaagcgcg agagctcgcc gtcgcacacc aggtcgatgc cggcctgctc ctgggtgtgc
      661 agcaccaccc gcgtcgcatc gatcagcgcc tggcgcgtcg ggtgggcggc aagccaggcg
      721 ggcaccggat aggagccgac gacagtggtc ttgatcgcgg gccgggacgt ggccatcgcc
      781 tgttgtccgc ctggttggcc tcgccgcgaa ccttagcgcc cggcgcgaag cctcggccag
      841 ccaattcgcc ttgacgctcg gcggacgggg cttgcatgct cggcgcgcat tcggcgagcg
      901 gaggagtctg acatgcccag gctgctggcg atgctggcgt tcgcgctgct gctcggcggc
      961 gcggcgcggg ccggcgacta tccgtggcag ccgacccggc cgatcaccat cgtcgtgccg
     1021 tggtcggcgg gcgggtcgac cgaccaggtg acgcgcgtgc tcgccggcga gctcgagctg
     1081 gcgctcggcc agaaggtggt ggtgatcaac cagcccggcg cctcgggctc gatcggcaag
     1141 aggaacgtct ggcaggcgcc gcatgacggt tacacatggg ccgcgggcgc gcccaagatg
     1201 ctcggcacct acaagctggt cggcctgttc gacacccgca tcgaggactg gcggctgtat
     1261 ctcgacgcgg tcaccccgac cctgatcggg gtcaaccccg acacgcccta tcaggacatg
     1321 ggcgatctgc tggaggcgat gcgcgcgcgt ccgggcgaga tttcggtcgc caccgccggc
     1381 gtcggctcgt cgggccacac caccatggag gcgatcgccc aggcgaccgg gatcaagtac
     1441 cgcaacgtct cctacgacgg cggcaacccg gcggtgatcg ccgccgtctc aggcgagtgc
     1501 gaggtcacca cccagctgtc gaccgagcag atcgagatgg cgcgcgccgg gcgcctgcgc
     1561 atcctggcgg tggtcgccga ccacgccgtg accctcgagg ggctcggcac catcccgccg
     1621 gtgaccgact ggaccgggcc gatccccaac acgctcaccc atttcggcat cttcgccccc
     1681 gccgacctgc cggccgaggt gatcgagacc ttcgacaagg tctgggagac gcggatcaag
     1741 acctccaagg cgctgcgcga ttacgccgcc gagcgcggca ccgtgttcga gaccgccttt
     1801 ggcgcggccg cgcagaccgc ggtgtttccg acggtccagg cggacgcgtg gacgctttac
     1861 ggcgcgggac ggaccaccat gtcgcccgac gaactcggca tccccaagcc ctgaccgccg
     1921 cgggccggcg gcggacgcga tggtcaaggc tgacttcgtc accgggctcg ccttcctcgc
     1981 gctcggcatc gcggcgctga tcgagtcgtt acgcatgccg cggttcgaga ccctggcgat
     2041 caacccctac accgtgcccg gcatcgtgcc cgggctgctg ggcgtggtga tcgcggcgct
     2101 cggcgcgatg cttgcgctgc gcgcggcgcg ctccggcggc tggcgggtgg cgcccgcgct
     2161 cgcccgcctc gccacgctgc accggagcga aggcgcgcaa aggctcgcgc tcgccctcgc
     2221 gctgaccttc ggctacgccg ccggcctgct cggccggctg ccgttctggc tggcgagcgc
     2281 gctgttcgtg ttcgccttca tcgccctgtt cgagtggcgc gcgcacattg aggcgaaggc
     2341 acgatggcgc gcgctcgcct tcgccgccgg cgaggcggcg gtcgcctccg cggcggtgac
     2401 gctggtgttc cgctacctgt tcctggtcaa cctgccgtga ccggcgggct tgccgacctg
     2461 gtcgcggcgc tcgtcggctt ccttgcgccg gaacccttgt tcagcgtgct gtgggcgacg
     2521 ctcctcggca tcgcggtcgg catgctgccg gggctcaccg ccaccctcgg cgtcgcgctg
     2581 atgaccacgc tcaccttccg catggacgcc ggccaggcga tcctgatctt gatctgcatg
     2641 tatgtcggcg cgatctacgg cggcagccgc agcgccatcc tgctcaacat cccggggacg
     2701 ccggcgagcg cagccactac cctcgacggc ttcccgctgg cgcgtgcagg ccgggccggc
     2761 ggcgccatgg cgaccgcgac ctcgggctcg gtgctcggca ccttgatcgg catggtgttc
     2821 ctggccgcgc tcgcgccggt gctggccgag ttcgcgctcg acttcggcgc ctacgagttc
     2881 ttctggctgg cgctgttcgg ggtcgtcatc tcgggccacc tgacagcgct cggcgatcca
     2941 ctgaagggct ggctcgccgg gttcctggga ctgctgctgg cgatggtcgg ccaggagggc
     3001 atccacgcct acgagcgctt cagcttcggc tcgaccgacc tcgccggcgg gctcggcctg
     3061 ctgccggcgc tggtcggcgc gttcggcttc gccgagatcc tgatcgtgat gaagagcccg
     3121 gcctacgagg tggtgcgcgg caaggtcgac tcggtgatcc cgcgcatcgg cgaggtgctg
     3181 cgctatcgct ggacgatcct gcgctcgggc gtgatcggca ccggcatggg cctgatcccg
     3241 ggggtcggcg aggacatggg cgcgtgggtc tcctacgccg ccgcgcgccg cctcagccgc
     3301 gagcgcgccc aattcggcaa gggctcgatc gagggcctga tggccgccga gaccggcaac
     3361 agtgccgcgg tccccggcgc gatcatcccg gtgctcaccc tcgccatccc gggctcggcg
     3421 ccgtcggcgg tgctgctggc ggcgatgttc atccacggcg tgcgcccggg gcccttgatc
     3481 atgatcgagt tcccgggctt cgttcaccag gtggtggcga tggtgttcct ggccagcctc
     3541 gcgatcctgt tgtggggaat cgtgctcacc cggccgttgc tcaccgtgct cagggtgccg
     3601 cgcgagcgca tgatgccggt ggtgttcgtg ctgtgcacga tcggctcgtt cgccatcgcc
     3661 gggcggctgt tcgacgtctg ggtgatgctc ggcttcggcg tgctcggctt cgccttgcgc
     3721 cagatggact atccgatggc gccgctggtg ctcggcatcg tgctcggcga catcctcgac
     3781 aagagcctgc gccgcggcct ggtgctgagc gacggcgacc cctcgccgtt cttcgcccgc
     3841 ccgatctcgg ccgtgctcgc ggcggtgacg ctggcgacca tcgcgttcag cattcccgtg
     3901 gtccgccgcg cggtcgggcg cgtgctggga ggagcgacgt gaacatcgcg ctgtgcaacg
     3961 aggtgatccg cgagctcgag ttcccggccc agtgcggctt cgccgccgcg ctcggctatg
     4021 acgccatcga gctcgcgccg ttcacgctcg ggcccgagcc gcacctgatg ggcgcgggcg
     4081 agcgcgcccg cctgcgccgc gccgccgccg atgccggcat cgccatctcg agcctccact
     4141 ggctgctggt ggtgccgaag ggcttgtcga tccacacccc cgacgacgcg ttgcgcgcac
     4201 gcacgatcga ggtcatgcgc cgcctgatcg ggctgtgcgc cgacctcggc ggcacggcga
     4261 tggtgcacgg ctcgccaatg gcgcgccgga ttcccgacgg cgccgatgcg gccgagtacc
     4321 gcgcccgcgc cctcgacacc ttcgccgccg tcgccgccga tgccgaggcc gcgggcgtgg
     4381 cctattgcat cgagccgctg gctgcgcgcg agaccaactt cgtcaatacg gtcgccgagg
     4441 ccgcggcaat ggtcgaggcg atcggcagcc cggcgctgcg caccatgatc gacacctgcg
     4501 cggcggccca ggccgaggcg ctgccggtgg ccgagctgat cgaccgctgg ctgccgaccg
     4561 ggctgatcgg ccacatccag gtcaacgacc gcgaccagtc cggcccgggc cagggggcgg
     4621 actcgttcgc gcccgctttc gccgcgctga ggcgcaatca ctatcgaggc acggtcgcgg
     4681 tcgagccgtt cgtctacttt cccgacgggc ccgcggcggc ggcgcgcgcg atcggctata
     4741 tacggggcat cctcgaggcg ctcgactggc gcgaccaata gagagcgaca tgcatgcggc
     4801 cgcaccccct cccaaccttc ccccgcccgc ggggcagggc gattgcatat ggcgaaggtc
     4861 ccgggcggtg agccgccctt aggcacgcgg aggcgcggag cacgcggaga aggcagaaag
     4921 gaaaaccaca gagtgcaaag aggccacaga agtaggtttc ctctgtgtcc tctgtgtcct
     4981 ctgtgtcctc tgtggttgat cccgaccacg ccccttccgc gtcctccgcg cctccgcgtg
     5041 aggaaaatga cacccggcca tccgacactg cccgaacaag caattgccgc atatgcgatt
     5101 gccctggcga gcggggggag gcaggtgggg ggtgcatctc ggtgccggtc atcatgataa
     5161 gtcccgatcg cgtctacgcc gcggcctcga gcgcgggcac caggtcgacg atggcgatgt
     5221 cggtccgcac gcgctgggtg gtgccgtcct ggaaggtgat ggtggtatcg acgtggaccc
     5281 ggtcgcggcc ggcgtacatc gcctcgatca gctcgccgta gcggcgcccg accacctcgc
     5341 ggcggacc
//
LOCUS       DASWHU010000135         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_426714, whole genome shotgun sequence.
ACCESSION   DASWHU010000135 DASWHU010000000
VERSION     DASWHU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426714"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1371
                     /locus_tag="VGB88_04930"
     CDS             268..1371
                     /locus_tag="VGB88_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9646819.1"
                     /translation="MVDRVDTVVVGAGAIGLAVARRLALAGREVVVLEAADSIGTGTS
                     SRNSEVIHAGIYYPTGSLKARLCVAGRRALYDFCASHGVPHRRCGKLIVATDDAQVGQ
                     LEALTRTAADNGVRDLRMIERAHALRLEPELRCAAALMSPSTGIIDSHALMLAYQGDA
                     EDRGAAIAFRTPVRRGEVRPDGLLIEAGDGTAVIAARLVNCAGLGAEALARAIAGLAP
                     ETVPTRYLARGVYFTLSGRSPFRHLIYPVPEPGGLGVHVTLDMAGQVRFGPDVEWIDE
                     VDYTVDAARAVRFYPAIRRYYPSLADGALQPGYAGIRPKVVPPGAPPGDFMIQGPDEH
                     GVAGLVNLYGIESPGLTASLAIADLVAAKLGVA"
     gene            complement(1381..2505)
                     /locus_tag="VGB88_04935"
     CDS             complement(1381..2505)
                     /locus_tag="VGB88_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9646820.1"
                     /translation="MADQAVVAALRQGAEAWNAWRGAGEPAMVDLVRADLGRCDLRRA
                     DLSGVNLAGAMLDGARLMGADLARANLAGCSLRQANLGEARLDGATAVSADFTESDLR
                     WARFEGANLTKARFYGADLTGTSLARAQMFRADLRWANLAEAVIADTNLSDADLYHAD
                     LRAARLCGVDLTGARLDLATLAEGTVSGCRVYGVSAWDVKLEGTEQADLVITPAGEPE
                     ILLDDLDAAQFVNLLLHSASLRHHVRAIETRMVLVIADRGSERAGRGKRLRHAVAAAG
                     WRPLFYDPATAEPEAAGRMCAALLRICPVAVAELTVVAPFAKSLLDAVAQQDRRLIPL
                     LPAADDDPSRLVEVAGRESLLLLAVDDTSLDRITRMLARL"
     gene            complement(2870..4939)
                     /locus_tag="VGB88_04940"
     CDS             complement(2870..4939)
                     /locus_tag="VGB88_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX9646821.1"
                     /translation="MSVIQRSAGVAVAALAAAFMAGEPAAAQELALKRVLLSSGGVAY
                     FEHEAVVEGDAELALDVRLDQVDDVLKSIVVFDDKGGVGAIRLPGREPLVQLFRDLPF
                     GPDALESPVRLLSQLKGAEVSVEGARGLSGRLVGVDAETVRLPDDGGTVTRHRVSLIT
                     ATGIRQFVLEETDSVRFDDPVLQGQVEAALEGISRHRVRDRRTLAIDAPGEGRRTVRV
                     GYVVAAPLWKASYRLSLAGDGKTGHLQGWAVIENMSGRPWTAVELTLASGNPVTFRQA
                     LYTAYYVDRPEVPVEVLGRVLPPPDTGAVVLEGGRAGGEAEAQSKTAARRAAVTESEE
                     MAVGAMADVMAEAPAPMEMPSTVAQAAITAAAEEATTQVTFRIAEPITVGAGESLLVP
                     VIDRDVPSAPLALYQPSTHPRHPLAAVRLDNATDTGLPPGVLTLYERGAGGANYIGDA
                     RLAPLPSGDSRLLSFAVDQNLLVDRESESASQIVRGSISRGLLRLSVRQRETTTYRIK
                     SAAKEARALVIEHPRRPGWDLAEPEIKEPELTETAYRIPGEIDAGGTATVAVALERTT
                     LEQVAILDLPVDRMQAYASARELSPEIRAAFERLAGLRAEVERHRSTLARIEQDRSVI
                     YQDQERLRDNLGRVPRDSDLYNRYLAKMSEEEDRLDGLAGEADTAQAALEDAEAALAD
                     AIAKLDL"
     gene            complement(5058..>5559)
                     /locus_tag="VGB88_04945"
     CDS             complement(5058..>5559)
                     /locus_tag="VGB88_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9646822.1"
                     /translation="AQRHGMVMWDEIFAEVARDFPDVATRRILVDACAAAMVLRPGDF
                     DVVVATNLHADILSDLAGALSGSLGMAASANLDPERRAPSMFEPVHGSAPDIAGRGIA
                     NPLAAVASGAMMLDHLGEAEAARRLAAAIERVAREGRALTPDLGGTATTDQLAEAVIA
                     ALEQGN"
BASE COUNT          766 a         2012 c         1999 g          782 t
ORIGIN      
        1 ccaccttcgc cgagtggccc gagcagtacg gctggtggct ggtgcgccgc gcctcgtcga
       61 gcgagttcta ccggcccgag gccgaagccg cggagtgagg gcgcgtaaat gaaccggctg
      121 ccgtccttcg agacgctcgc tccgctcgct cctcaggacg aggatgcatt gaaaatcaag
      181 aaaatacctc accctgagga gcgcccggag ggcgcgtctc gaagggtgcc ccgaaagata
      241 cgcaagctct gagagccgcg cgcgccgatg gtcgaccggg tcgacaccgt ggtggtcggc
      301 gcgggcgcga tcggtctcgc cgtcgcccgc cggctggcgc tggccgggcg ggaggtggtg
      361 gtgctggagg ccgcggactc gatcggcacc ggcaccagct cgcgcaacag cgaggtgatc
      421 cacgccggca tctactaccc gaccggcagc ctgaaggcgc ggctgtgtgt cgccggccgg
      481 cgcgcgctgt acgacttctg cgccagccac ggcgtgcccc accgccgctg cggcaagctg
      541 atcgtcgcca ccgacgacgc ccaggtcggg cagctcgagg cgctgacgcg cacggccgcg
      601 gacaacgggg ttcgcgacct gcgcatgatc gagcgcgcgc acgcgctccg gctcgagccc
      661 gagctgcgct gcgccgcggc gctcatgtcg ccctcgaccg ggatcatcga cagccatgcc
      721 ctgatgctcg cctatcaggg cgacgccgag gaccgcggcg cggcgatcgc cttccgcacg
      781 ccggtccggc ggggcgaggt gcgcccggac gggctgttga tcgaggccgg cgacggcacc
      841 gcggtgatcg cggcgcgcct ggtcaactgc gccggcctcg gcgccgaggc gctggcgcgc
      901 gccatcgcgg gcctcgcgcc cgagaccgtg ccgacgcgct atctggcgcg cggcgtctac
      961 ttcaccttgt ccgggcgcag cccgttccgc cacctgatct atccggtgcc cgagcccggc
     1021 ggcctcggcg tccacgtcac cctcgacatg gccggccagg tgcgcttcgg ccctgatgtc
     1081 gaatggatcg acgaggtcga ttacacggtg gatgcggcgc gcgccgtgcg cttctatccg
     1141 gccatccgcc gctactaccc gagcctcgcc gacggcgcgc tgcagccggg ctatgccggc
     1201 atccggccca aggtggtgcc gcccggcgcg ccgcccgggg atttcatgat ccagggcccg
     1261 gacgagcacg gcgtcgccgg cctggtcaac ctgtacggga tcgagtcgcc gggactgacc
     1321 gcctcgctcg ccatcgccga cctggtcgcg gccaagctcg gcgtcgcctg aggtacgcgc
     1381 ctacaggcgc gccagcatgc gcgtgatgcg gtcgagagag gtgtcgtcga ccgcgagcag
     1441 cagcagcgac tcgcggccgg cgacctcgac caggcgcgac gggtcgtcgt ccgccgccgg
     1501 cagcagcggg atcagccggc ggtcctgctg ggcgacggcg tcgagcaacg acttggcgaa
     1561 cggcgccacc acggtcagct cggcgaccgc gaccgggcaa atcctgagca gcgccgcgca
     1621 catccggccc gcggcctcgg gctcggccgt ggccgggtcg tagaacagcg gccgccagcc
     1681 ggccgccgcg accgcatggc gcaaccgctt gccgcgcccc gcgcgctccg atccgcggtc
     1741 ggcgatgacc agcaccatgc gcgtctcgat cgcgcgaaca tggtgcctga gcgaggcgct
     1801 gtgcagcagc aggttgacga actgggcggc gtcgaggtca tcgagcagaa tttccggctc
     1861 gccggcgggc gtgatcacca ggtccgcctg ctccgtgccc tcgagcttca cgtcccaagc
     1921 cgagacgcca tagacgcggc agccgcttac ggttccctcg gccagggtgg cgaggtcgag
     1981 ccgggcgccg gtcaggtcga ctccgcacag ccgcgccgcg cgcaggtcgg cgtggtagag
     2041 gtcggcatcg gacaggttgg tgtcggcgat caccgcctcg gccaggttgg cccagcgcag
     2101 gtcggcgcga aacatctgcg cgcgcgccag cgacgtgccg gtgagatcgg cgccgtagaa
     2161 gcgcgccttg gtcaggttgg cgccctcgaa ccgcgcccag cgcaggtctg actcggtgaa
     2221 atcggcactc accgcagtgg cgccgtcgag ccgcgcctcg ccgaggttcg cctggcgcag
     2281 cgagcagccg gcgaggttgg cgcgggcgag gtcggcgccc atcaggcgcg cgccgtcgag
     2341 catcgcgccg gccaggttga cgccgctgag gtcggcgcgg cgcaggtcgc agcggccaag
     2401 atcggcgcgc accaggtcga ccatcgccgg ctcgcccgcg ccgcgccacg cgttccacgc
     2461 ctccgcgccc tggcgcaagg ccgcgaccac cgcctgatcg gccatgtctt tgcatgcgcg
     2521 caccgactcg ccggccgcgc gctcccgagg tgtcggtcga tactgaatgt agagcgaatg
     2581 ccggggtcgt tgactcgctc cgccccacgc tccacccgcc tccccccgca agcgggaggt
     2641 caagaagatg aaaccctacc ccgcgcgcga gggagggatt gggtgggggt gacgctacga
     2701 tcgagcagac tctgagtcag tcgtgctgac accgcgccgc gacgacgccg gggcgcgcct
     2761 tccaggccgc gcgttttctt ctagtcaagt acgagaattg ttcgacaata tattgtgata
     2821 tttgtactcg tcgatcgtcg ataaattccc ttcggcgcgt tgcagctggt cacaggtcga
     2881 gcttggcgat ggcgtcggcg agggcggcct ccgcgtcctc gagcgccgcc tgcgcggtgt
     2941 cggcctcgcc ggcgagacca tcgagccggt cctcctcctc ggacatcttg gcgaggtagc
     3001 ggttgtacag gtcgctgtcc ctcggcaccc ggccgaggtt gtcgcgcagc cgctcctggt
     3061 cctgatagat gaccgagcgg tcctgctcga tgcgggcgag cgtggagcga tgccgctcga
     3121 cctcggcgcg cagaccggcc aggcgctcga aggcggcgcg gatctcgggc gacagctcgc
     3181 gcgcgcttgc ataggcctgc atgcggtcga ccggcaggtc gaggatcgcc acctgctcga
     3241 gcgtggtgcg ctcgagcgcg accgccaccg tggcggtgcc gccggcgtcg atctcgcccg
     3301 ggatgcgata ggcggtctcg gtgagctcgg gctccttgat ctcgggctcg gcgaggtccc
     3361 agcccggcct gcgcgggtgc tcgatgacca gcgcgcgcgc ctccttggcc gcggacttga
     3421 tacgataggt ggtggtctcg cgctggcgca ccgacagcct caagaggccg cggctgatcg
     3481 agccgcgcac gatctggctc gccgactcgg actcgcggtc gaccagcagg ttctggtcga
     3541 cggcgaagct cagcagccgc gaatccccgg acggcaacgg cgcgagccgc gcgtcgccga
     3601 tatagttggc gccgcccgca ccgcgctcgt agagcgtcag cacgccgggc ggcaggccgg
     3661 tgtcggtggc gttgtcgagc ctgaccgcgg cgagcgggtg gcgcgggtgg gtcgagggct
     3721 gatagagcgc gagcggcgcc gacggcacgt cgcggtcgat caccggcacc agcaatgact
     3781 cgccggcgcc gacggtgatc ggctcggcga tgcggaaggt gacctgcgtg gtcgcctcct
     3841 cggccgcggc cgtgatcgcg gcctgcgcca ccgtgctcgg catctccatg ggcgccggcg
     3901 cctcggccat cacgtcggcc atcgcgccga ccgccatctc ctcgctctcg gtgaccgcgg
     3961 cacgccgcgc tgcggtcttc gactgcgcct ctgcctcgcc gccggcgcgc ccgccttcga
     4021 gcaccacggc gccggtatcc ggcggcggca gcacccggcc cagcacctcg accggcacct
     4081 cggggcggtc gacgtaatag gcggtgtaca gcgcctggcg gaaggtgacc gggttgcccg
     4141 aggccaaggt cagctcgacc gcggtccacg gccggccgct catgttctcg atcaccgccc
     4201 agccttgcag gtggccggtc ttgccgtcgc cggcgaggct caggcggtag ctcgccttcc
     4261 acaacggcgc cgcgaccacg tagccgaccc ggaccgtgcg ccggccttcg cccggcgcgt
     4321 cgatcgccaa ggtcctgcgg tcgcgcaccc gatggcgcga gatgccttcg agcgcggcct
     4381 cgacctgccc ctggaggacc ggatcgtcga agcgcaccga gtcggtctcc tcgagcacga
     4441 actggcggat gccggtggcg gtgatcaggc tgacccgatg gcgggtgacg gtgccgccgt
     4501 cgtcgggcag gcgcacggtc tcggcatcga cgccgaccag gcggccggag agcccgcgcg
     4561 cgccctcgac cgagacctcg gcacccttga gctggctcag cagcctgacc ggggactcga
     4621 gcgcgtccgg cccgaacggc aggtcgcgga acagctgcac gagcggctcg cgcccgggca
     4681 ggcggatcgc gccgacgccg cccttgtcgt cgaacacgac gatgctcttg agcacgtcgt
     4741 cgacctggtc gaggcgcacg tcgagcgcga gctcggcatc gccctcgacc accgcctcgt
     4801 gctcgaaata ggcgacaccg cccgaggaca gcagcacccg cttcagcgcg agctcctgcg
     4861 ccgccgccgg ctcgccggcc atgaaggcgg cggccagcgc ggccaccgcg acgccggcac
     4921 tgcgttggat cacggacatt tcactgtttc ccacctggtc acgaaatcat gtcgtctaaa
     4981 tgcggtacgc agggactgtg gcgttcgggt gatcgaatcg cggcaatttc gtgccgaagg
     5041 cgccgccgcg gggcggccta gttcccctgc tcgagcgccg cgatcaccgc ctcggcgagc
     5101 tggtcggtgg tggcggtgcc gccaaggtcc ggcgtgagcg ccctgccctc gcgcgccacc
     5161 cgctcgatcg cggcggcgag ccggcgcgca gcctcggcct cgccgagatg gtcgagcatc
     5221 atcgcgccgc tggcgaccgc ggccagcggg ttggcgatgc cgcgcccggc gatgtcgggc
     5281 gccgagccgt ggaccggctc gaacatcgac ggcgcgcgcc gctcggggtc gaggttggcg
     5341 ctcgccgcca tgccgaggct gcccgagagc gcgccggcga ggtcggacag gatgtcggcg
     5401 tgcaagttag tggcgacgac cacatcgaag tcgccgggcc tgagcaccat cgccgccgcg
     5461 caggcgtcga ccaggatgcg ccgcgtggcg acatccggaa agtcgcgcgc gacctcggcg
     5521 aagatctcgt cccacatcac catgccgtga cgctgggca
//
LOCUS       DASWHU010000136        14546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_427770, whole genome shotgun sequence.
ACCESSION   DASWHU010000136 DASWHU010000000
VERSION     DASWHU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14546
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427770"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VGB88_04950"
     CDS             complement(<1..655)
                     /locus_tag="VGB88_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9646823.1"
                     /translation="MFEVRDPAEARRRYDAGTAVFAPATPPLDHVADDVLPGPAGPLP
                     IRIYRPRGLGARLLPTVAFFHGGGWVFGNRDSHDAVCRILAHQAGALVVSLDYRLAPE
                     HKFPAAFDDCVAATRWLLAQAGELGGDAARVAVAGDSAGGNLAAATCLALRDAGGPAP
                     VFQLLIYPAVDLAADSDSHRAFADGYLLTAKAIAWCTDSYLAGAEEVSDWRASPLRAA
                     "
     gene            944..1135
                     /locus_tag="VGB88_04955"
     CDS             944..1135
                     /locus_tag="VGB88_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX9646824.1"
                     /translation="MGWLAPLRYHLRRLVKDETGATAIEYGLIAALISVVIIAALTLV
                     GTELNQTFGKIGSELSSVN"
     gene            1327..1719
                     /locus_tag="VGB88_04960"
     CDS             1327..1719
                     /locus_tag="VGB88_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646825.1"
                     /translation="MSTGAAAADPLVYFDGLVDGDRQSFRMYLALTAALVAAGIALLA
                     VGILFPASDEAEFAGLQDLAVKLGGLCISSLGTIPLSQCLTRRDRLIAAQGLRAFLAA
                     RLQAAGSPEQALAELYGEIARMYQVRMS"
     gene            1729..2460
                     /locus_tag="VGB88_04965"
     CDS             1729..2460
                     /locus_tag="VGB88_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646826.1"
                     /translation="MSTQAPGLDEIQARIARQERRRLLRTVLAVAVPVAVAVAYLLYT
                     LDAIDQAETDLHATRVALVAATAEVESKRRAVAALEAQVASLETQLADVSARLSEAVG
                     MRNQILEFDWAEAKFLASQSERLAKLVFVINDLHQRGVGWGFENTPEDGFTSPGLAGY
                     ALQKIGRLPDDLEPAGALAQLPVEQGEAELGDLIFYEPSYYLFYLRDQDGQPFVLGMT
                     PFGILPLQVDFGPERLGILRTGLAQ"
     gene            complement(2488..3537)
                     /locus_tag="VGB88_04970"
     CDS             complement(2488..3537)
                     /locus_tag="VGB88_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9646827.1"
                     /translation="MARTAVVAGALGVAGRALVEHLEAEPAWRVVGLSRRAPDFASRA
                     EFIRVDLLDAADCRAKLAPLGEATHLFFAAYQAAPNPAAEVAPNLAMLVNLVEALESI
                     APGLAHVQVIHGSKWYGNHLGPYRTPAREDDARHLPPNFYYDQQDWLEAHQRGQRWTW
                     SALRPHGLLGLSIGSPMNQLTALGVFGSIVTHLGLPLCWPGKPGAFERVYQFTDAGHL
                     AQAMSWAATTPACANQAFNLTNGDFVRWRNLWPAVARFFGTEPGPVRTLPLEAVMADK
                     EPLWREIVARRGLKPYRVGELTNWRFADFVYGTEYDQMSSLTKARRAGWHHDLDSEAA
                     LLGLLARLRAERLIP"
     gene            complement(3546..3962)
                     /locus_tag="VGB88_04975"
     CDS             complement(3546..3962)
                     /locus_tag="VGB88_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9646828.1"
                     /translation="MVDLSGGCNCGATRYRITSAAKRVSHCHCTICRRAGGAVAGTFA
                     TFDAAAVTWQGAEPRLYRSSDFAFRRFCGACGSQLAFGYDALPELVSITVGTLDSPEL
                     TPAERHDFAAERIPWVQLDAQLPATPRWWDPPPGKE"
     gene            4053..5396
                     /locus_tag="VGB88_04980"
     CDS             4053..5396
                     /locus_tag="VGB88_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646829.1"
                     /translation="MTADNVVKIGPQQASRRVPPAPADLKATGVAQNFLEGLMLKTMY
                     ALGLESTPELRSHLKLPDKVLNALFEEARRKGLMTVGDMSEFVARVELRFHLTDRGRA
                     RAIEALDQSQYVGAAPVSMADFIAQTKAQSVANEQITPAGLAACFGGMSMSEQLAEQI
                     GAALNHGHSMLLYGPPGNGKSIYSEAIVRSFAQTVFIPHAVIVDGQVISLFDAAVHEP
                     IGDAQAGQPGAETAPDPRWVHCRRPAVIAGGELTMEMLELVYDPNTKSYEAPLQLKAT
                     GGVFIIDDFGRQAVDPARILNRWIVPLERKHDYLALRSGKKVAVPFDELLIFSTNLDP
                     NGFMDTAMMRRIHYKIEIGPPEREEYLAILAEECRKAGLALPADIVSYLCDEFYPQGE
                     VQIARFHPKWIVDHVTSGCAYDGVAPVLDRDRVARAMNNLFVPRAAQASRAVRAP"
     gene            5430..7961
                     /locus_tag="VGB88_04985"
     CDS             5430..7961
                     /locus_tag="VGB88_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9646830.1"
                     /translation="MQGFRRSRRARTETGQLTAVLGPTNTGKTHLAVERMLGHQSGMI
                     GCPLRLLAREIYDRAVRTRGAGQVALVTGEERIVPARPRYFVCTVESMPLERAVEFLA
                     VDEIQLIADPERGHVFTDRLLNARGLSETMFLGAATAAPMLRRLLPAAELVSRPRFSK
                     LDYAGSRKLTRLPARTAVVAFSATEVYQIAELLRRFRGGAAVVMGALSPRTRNAQVGL
                     YQAGEVDYLVATDAIGMGLNMNVDHVAFARLDKFDGREGRPLTAAEIAQIAGRAGRHT
                     RDGSFGTTHDAGPLDAALVAAVEEHRFEPITRIYWRNGQLDFASLGALIRSLDTPPPL
                     DELIRPREADDLASLRALAAMPDIARLARGWDAVALLWDVCCIPDFRQTMAEAHVRLL
                     ADIYRHLMGPSSLLPAAWVQRLVDRLDHTDGDIDTLAMRIAHIRTWNYVGHRPGWVAD
                     PAALQDRARDIEDRLSDALHDRLTQRFVDRRTTVLQRRLKQQRALVAAVKRSGEVVVE
                     GEHVGRLDGFDFVPDDAAAWSRARVLRAAVRRALEPEMRRRAGALEAAANEAFALGPD
                     GSLRWQGATVARLVAGAALAAPGLALTVGDLVEEPLRRRIEKRLGAWLRWHVGERFAP
                     LTRAREAPLAGPARGLAFQLAEALGSVPRANVAEQIAALDARARKDIARLGIRLGRES
                     AFMPAMLKPRPVALRALLWCVAERMRPVAPPAGQVSLALDPALPERFYAAIGYRVLGA
                     RALRVDMVERLAGELRRRARTGPVTPDAALLNLAGCAGEGFAGVVAGLGFKALPVAAD
                     GAVAYVPMRAPRREAAGSRPRPPRRRGPDGESPFAALAALKRRRR"
     gene            8003..8350
                     /locus_tag="VGB88_04990"
     CDS             8003..8350
                     /locus_tag="VGB88_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX9646831.1"
                     /translation="MRLDRWLWCARFFKSRTQAAKLCAGSRVRRNRRVLTKASATVRV
                     GDVLTFPQGARVRVVRVLALPGRRGPAASALALFDDLSPPPAPQPGGRPAGSGRPTKA
                     DRRALEHLRDAEP"
     gene            8418..8876
                     /locus_tag="VGB88_04995"
     CDS             8418..8876
                     /locus_tag="VGB88_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX9646832.1"
                     /translation="MIERIAGLLRRLEAGPQVPHSAADMELAACALLVEAACMDGTFA
                     PEERATIERLIGARFGLDAGAAAGLVGEAEGVVAEAPDLFRFTNAIKSSFSEAERIEL
                     IEMLWEVAYADGTVHAYEDNLLRRIAGLIYVTDRDRGLARARVLERLGRS"
     gene            8977..9318
                     /gene="fdxA"
                     /locus_tag="VGB88_05000"
     CDS             8977..9318
                     /gene="fdxA"
                     /locus_tag="VGB88_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX9646833.1"
                     /translation="MTYVVTEACIKCKYMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPAEAILPDSEGELDKWLALNSEYADKWPNITRKGPAPADADDFKGVPGKFDQ
                     YFSANPGKGSG"
     gene            9751..10251
                     /locus_tag="VGB88_05005"
     CDS             9751..10251
                     /locus_tag="VGB88_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX9646834.1"
                     /translation="MAKKLEYRPGDLVVYPCHGLGEIVGVETSEFGGVELRLYVISFE
                     KDRMILRVPVDKASGSGVRRVSTRDEMQKALTTLRGRARVKRTMWSRRAQEYEAKLNS
                     GNPISIAEVVRDLHRAADQPEQSYSERQLYEAALDRLSREYAAVENIEQSVATKRLED
                     VLKKAA"
     gene            10310..11116
                     /locus_tag="VGB88_05010"
     CDS             10310..11116
                     /locus_tag="VGB88_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646835.1"
                     /translation="MALDDRVKFGTVERARRIWAVGAVHGEFDRLSALHQRLARGFRA
                     GDRLVYLGNYLGRGARIVDTIDEMLLFRRALMARFSLFETDVQFLRGSQEEMWQKLLQ
                     LQLAQSPAQVFEWMITQGVEATLRAYGVNPDSGRMRCRDGPLALARWSLELREAIRAH
                     AGHEQLLSALRRAAYTDDGHILFVHAGIDTSRPLAAQTDSFWWGGGDFAGIVEPYEGF
                     SRIVRGFDPGHGGVDIGVVAATVDSGCGFGGPLTAICLERTGEVVEAIEG"
     gene            11783..12664
                     /locus_tag="VGB88_05015"
     CDS             11783..12664
                     /locus_tag="VGB88_05015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX9646836.1"
                     /translation="MAYVDTPQTQKANRRYINRSMKAALLTREREFELARAWREQGDE
                     RALHELVNAYGRLVIKLASRFRNYGLPMSDLVQEGNIGLLQAAARFEPEREVRFSTYA
                     AWWIRSAMQDFVLRNWSIVRTGTTAAQKSLFFNLRRLRAKHADDVDGWLSEEAKDRIA
                     DELRVRVNDVEDMEMRLAGNDQSLNACVSDASEDEWQDFMSDDRPSPEDQVRDMHDSA
                     TRSRWLQAALDELGERERTIIHERRLRDDPVTLEELGKALGISKERVRQIEHKALQKL
                     RASITRQSDEARRTTVA"
     gene            complement(12683..13498)
                     /locus_tag="VGB88_05020"
     CDS             complement(12683..13498)
                     /locus_tag="VGB88_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9646837.1"
                     /translation="MAAAAPVHAATPADALEPGGEGVVALVVDGDTLALADGTVVRLV
                     GLQAPKLALGRAGLSDWPLAEEARAALAELALGQRVTLRYGGRRSDRHGRALAHLVRA
                     DGVWIQDRMLARGMARVYSFADNRALVADMLASEAGARAALRGIWAHPFYRPRAADRL
                     DGAIGSFQLVEGTVEAAALVRGRLYLNFGADWREDFTVTVAPRLRPDFERAFAALGLD
                     SLEALAGRRVRVRGWIDRYNGPEIEATHPEQIELLADGRQTSRAAARGRGPSS"
     gene            13607..>14546
                     /gene="pip"
                     /locus_tag="VGB88_05025"
     CDS             13607..>14546
                     /gene="pip"
                     /locus_tag="VGB88_05025"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX9646838.1"
                     /translation="MRDLYPDIEPFATGRLPLEPPHVMYWEQSGAEDGVPVLFLHGGP
                     GAGATAANRRFFDSAHYRIIVYDQRGAGRSEPLGELAANTTPNLVEDIEALRDHLGIG
                     SWLVFGGSWGSTLALAYGESHPERCLALVLRGIFLCRRAEIDWFVNGIRRFFPEPWQA
                     FAGHLPEAEQGDLLAAYHRLLTDPDPAVHMPAARAWSRYEGSCSTLQPSPETVAEFGR
                     DRMALGLARIEAHYFLNDIFMPANHLLENVGRLRSIPGYIVQGRYDMVCPIETACDLV
                     RAWPEAELHVVQDAGHSSMEPGIRAKLVEIMDRLRDH"
BASE COUNT         2121 a         5145 c         5217 g         2063 t
ORIGIN      
        1 tggcggcgcg gagcggcgag gcgcgccagt ccgagacctc ctcggcgccg gcgagatagc
       61 tgtcggtgca ccaggcgatc gccttggcgg tgagcaggta gccgtcggcg aaggcgcggt
      121 gcgagtcgct atcggcggca agatcgaccg cagggtagat cagcagctgg aacaccggcg
      181 ccgggccgcc cgcgtcgcgc aacgcgaggc aggtggcggc ggcgaggttg ccgccggcgc
      241 tgtcgccggc caccgccacc cgcgccgcat cgccgccgag ctcgccggcc tgggccagca
      301 gccagcgcgt cgccgccacg cagtcgtcga acgcggccgg gaacttgtgc tcgggcgcca
      361 ggcggtagtc gagcgagacc accagcgccc cggcctggtg ggcgaggatg cggcagaccg
      421 catcgtggct gtcgcggttg ccgaacaccc agccgccgcc gtggaagaag gcgaccgtcg
      481 gcaaaaggcg ggcgccgagg ccgcgcgggc gatagatacg gatcggcaac ggtcccgccg
      541 gcccgggaag cacgtcgtcg gcgacgtgat cgagcggcgg cgtcgccggg gcgaacaccg
      601 ccgtgccggc gtcatagcgc cggcgcgcct cggccgggtc gcggacctcg aacacgcttg
      661 cgttcacggc cgtggcggcg agcaccgccc gacactgggg atcaaccggc atgcgccgag
      721 catagcagga tcggggccga tgggccgatc gcattatccc caagcgggct gccggaaatc
      781 ataagattga tgttgacagt actggtgatt tccctagaat tttcgagtca agcgccgggc
      841 aagccttggc agaatgcccg cacagcgctg gggcggtttg ggctcgagcg gtagggtttg
      901 ggtcacggcg atcaacactc ggggtgctat ggaggacttc atcatgggct ggctcgctcc
      961 gcttcgttac caccttcgtc gcctggtcaa ggacgagacc ggcgcaaccg ccatcgaata
     1021 cgggctgatc gcggccctga tctcggtcgt gatcatcgcc gcgctgaccc tggtcggcac
     1081 cgagctcaac cagaccttcg gcaaaatcgg cagcgagctg tcctcggtca actgaccgct
     1141 cgccaagccg atcgcggcgg agacacaact ccagccgccg tgcccccggg cccgccctgg
     1201 cccgggggta ttgattgcgt tcggccgtcc ccgcaggcgg gccggcgatc ggcttgaggt
     1261 cggcgcgcgt tcggaagaat atggcgggcg ttttgtgcgc gaacctgcgc ggccgaaggt
     1321 gggcccgtga gcactggggc ggcagcagcg gatccgcttg tatacttcga cgggctggtc
     1381 gatggcgacc gccagtcgtt ccgcatgtac ctggcgctga cggcggcgct ggtcgccgcc
     1441 ggaatcgcgc tgctcgccgt cggcatcctg tttccggcca gtgacgaggc cgagttcgcc
     1501 ggcctgcaag atctggcggt caagctcggc gggctgtgca tcagctcgct tgggaccatc
     1561 ccgttgagcc aatgcctgac gcggcgcgac cggctgatcg cggcccaagg cctgcgcgct
     1621 ttcctggcgg cgcgcctgca ggcggcggga tcgccggagc aagctctggc agaactctac
     1681 ggcgagattg ccaggatgta tcaggtgcgt atgtcgtgag gtcctaggat gagcacgcag
     1741 gcgcccggcc tcgacgagat ccaggcgcgc atcgcgcgcc aggaaagacg ccgcctgctg
     1801 cggaccgtgc tcgccgtggc ggtgccggtg gcggtcgcgg tggcgtacct gctgtacacg
     1861 ctggatgcga tcgaccaggc ggaaaccgat ctgcatgcga cgcgcgttgc gctggttgcg
     1921 gcgactgccg aggtcgagag caagcgccgc gccgtcgccg cgctcgaagc gcaggtcgca
     1981 agcctcgaga cgcagctcgc cgatgtcagc gcgcggctgt cggaggcggt cggcatgcgc
     2041 aaccagatac tcgaattcga ctgggccgaa gcgaagttcc tggccagcca gagcgagcgc
     2101 ctcgccaagc tggtgttcgt gatcaacgac ctgcaccagc gcggcgtcgg ctgggggttc
     2161 gagaacacgc ccgaggacgg cttcacctcg ccgggtctgg ccggctacgc gctccagaag
     2221 atcggccggc tgcccgacga cctggagccc gcgggcgcgc tggcccagct gccggtcgag
     2281 cagggcgagg cagagctcgg cgacctgatc ttctacgagc ccagctacta cttgttctac
     2341 ctgcgggacc aggacggcca gccgttcgtg ctcggcatga ccccgtttgg catcctgccg
     2401 ttgcaggtcg acttcggccc cgagcgcctg ggcatccttc gcaccggcct ggcgcagtga
     2461 gcgcccgcgg gcgatggcgg cgcgcggtca cgggatcagg cgctcggcgc gcagccgggc
     2521 gagcaggccg agcagcgccg cctcgctgtc gaggtcgtgg tgccagccgg cgcggcgcgc
     2581 cttggtcagc gacgacatct ggtcgtactc ggtgccgtag acgaagtcgg cgaagcgcca
     2641 gttggtgagc tcaccgacgc ggtacggctt gagcccgcgg cgggcgacga tctcccgcca
     2701 cagcggctcc ttgtcggcca tcaccgcctc cagcggcagc gtccgcaccg gccccggctc
     2761 ggtgccgaag aagcgcgcga ccgccggcca caggttgcgc cagcgcacga agtcgccgtt
     2821 ggtcaggttg aacgcctggt tggcgcaggc gggcgtggtc gccgcccagg acattgcctg
     2881 cgccaggtgg ccggcgtcgg tgaactggta gacgcgctcg aacgccccgg gcttgcccgg
     2941 ccagcacagc ggcaggccga gatgcgtcac gatcgagccg aatacgccga gcgcggtgag
     3001 ctggttcatc gggctgccga tcgacagccc gagcagcccg tgcggcctga gcgccgacca
     3061 ggtccagcgc tggccgcgct ggtgcgcctc cagccagtcc tgctggtcgt agtagaagtt
     3121 gggcggcagg tggcgcgcat cgtcctcgcg cgccggcgtg cggtacggtc ccagatggtt
     3181 gccataccat ttgctgccgt ggatgacctg gacgtgcgcg aggccgggcg cgattgactc
     3241 gagcgcttcg accaggttaa ccagcatggc gaggttgggc gcgacctcgg cggccgggtt
     3301 tggcgcggcc tgataggcgg caaagaacag gtgcgtcgcc tcgccgagcg gggcgagctt
     3361 ggcccggcaa tccgccgcat ccagcaggtc gacgcggatg aactcggcgc ggctggcgaa
     3421 gtcgggcgcc cgccgcgaca gcccgaccac gcgccaggcg ggctccgcct ccaggtgctc
     3481 gaccagcgcc cggccggcga cgccgagcgc acctgccacc accgcggtcc gtgccatcgc
     3541 cgcccctact ctttccccgg cggcgggtcc caccagcgcg gcgtcgccgg cagctgcgca
     3601 tcgagctgca cccaggggat cctctcggcc gcgaagtcgt gccgctctgc cggggtcagc
     3661 tcgggcgaat cgagagtgcc gacggtgatc gagaccagct ccgggagggc gtcgtagccg
     3721 aaggcgagct ggctgccgca ggcgccgcag aagcgccgga aggcgaagtc gctcgaccgg
     3781 tacagcctgg gctcggcgcc ctgccaggtc accgccgcgg catcgaaggt ggcgaaggtg
     3841 ccggccaccg ccccgcccgc ccgccggcag atggtgcaat ggcaatggct gacccgcttg
     3901 gccgccgagg taatccggta cctggtcgcg ccgcagttgc atccgccgct aaggtccacc
     3961 attttcctct ccgtgccagc cgcggcgcgg ccgcggcgcc ggcgggtcgt catcatagcg
     4021 cgcctgccgc ccacgcacag gcaggggatg acatgactgc ggacaacgtc gtcaagatcg
     4081 gcccccagca agcatcgcgg cgcgtgccgc ccgccccggc cgacctcaag gcgaccgggg
     4141 tggcgcagaa cttcctcgaa ggcctgatgc tcaagaccat gtatgcgctc gggctcgaga
     4201 gcacgccgga gctcaggtcc cacctcaagc tgcccgacaa ggtgctgaac gccctgttcg
     4261 aggaggcgcg ccgcaagggc ctgatgacgg tcggcgacat gtccgagttc gtcgcccgtg
     4321 tggagctccg cttccatctc accgaccgcg gccgggcgcg ggcgatcgag gccctcgacc
     4381 agtcgcagta tgtcggcgcg gcgccggtgt cgatggccga cttcatcgcc cagaccaagg
     4441 cccagagcgt cgccaacgag cagatcacgc cggccggcct cgccgcctgc ttcggcggca
     4501 tgtcgatgtc cgagcaactc gccgagcaga tcggcgcggc gctcaaccac ggccattcga
     4561 tgctgctcta cgggccgccc ggcaacggca agtcgatcta ctcggaagcg atcgtgcgct
     4621 cgttcgccca gaccgtgttc attccccatg ccgtgatcgt cgacggccag gtgatcagcc
     4681 tgttcgacgc cgcggtccac gagccgatcg gcgacgcgca ggcgggccag ccgggggctg
     4741 agacggcgcc cgatccgcgc tgggtccact gccggcgccc ggcggtgatc gccggcggcg
     4801 agctgaccat ggagatgctc gagctggtct acgaccccaa caccaagagc tacgaggcgc
     4861 cgctccagct caaggccacc ggcggggtgt tcatcatcga cgacttcggc cgccaggcgg
     4921 tcgacccggc gcgcatcctc aaccggtgga tcgtgccgct cgagcgcaag cacgactacc
     4981 tcgcgctccg gtcgggcaag aaggtggcgg tgccgttcga cgagctgttg atcttctcga
     5041 ccaacctcga ccccaacggc ttcatggaca cggcgatgat gcgccgcatc cactacaaga
     5101 tcgagatcgg gccgccggag cgcgaggagt acctcgccat cctcgccgag gagtgccgga
     5161 aggccggcct cgccttgccc gccgacatcg tctcgtacct gtgcgacgag ttctatccgc
     5221 agggcgaggt gcagatcgcg cgcttccacc cgaaatggat cgtcgaccac gtgaccagcg
     5281 gctgcgctta tgacggggtc gcacccgtgc tcgaccgcga ccgcgtcgcc cgggccatga
     5341 acaacctgtt cgtgccgcgc gccgcgcaag ccagccgtgc cgtccgcgca ccttgattcg
     5401 ccgcgtccat tgggcgattc tgtgaggtga tgcagggttt tcggcgctcc cgtcgtgcgc
     5461 gcaccgagac cggccagctg accgcggtcc tcgggccgac gaataccggc aagacccacc
     5521 ttgcggtcga gcgcatgctc ggccaccaga gcggcatgat cggctgcccg ctgaggctgt
     5581 tggcgcgcga gatctacgac cgcgcggtgc gcacgcgcgg cgccggccag gttgcgctgg
     5641 tcaccggcga ggagcggatc gtgccggcgc gcccgcgcta cttcgtctgc accgtcgaat
     5701 cgatgccgct cgagcgtgcg gtcgagttcc tggcggtcga tgagatccag ctgatcgccg
     5761 atcccgagcg cggccacgtg ttcaccgacc ggctgctcaa tgcgcgcggg ctgtcggaga
     5821 ccatgttcct cggcgcggcg accgcggcgc cgatgctgcg ccggctgctg cccgcggcgg
     5881 agctggtctc caggccgcgg ttctcgaagc tcgactatgc cggcagccgc aagctcaccc
     5941 gcctgccggc gcgcaccgcg gtggtcgcgt tctcggccac cgaggtctac cagatcgcgg
     6001 aactgctgcg ccgcttccgc ggcggcgcgg cggtggtgat gggcgcgctc agcccgcgca
     6061 cgcgcaacgc ccaggtcggg ctctaccagg ccggcgaggt cgactacctg gtcgccaccg
     6121 acgctatcgg catgggcctc aacatgaacg tcgaccacgt cgccttcgcc cgcctcgaca
     6181 agttcgacgg ccgcgagggc cgcccgctca ccgccgccga gatcgcccag atcgccggcc
     6241 gcgccgggcg ccatacccgc gacggcagct tcggcaccac ccacgacgcg ggcccgctcg
     6301 atgcggcact ggtggcggcg gtcgaggagc atcggttcga gccgatcacg cgcatctact
     6361 ggcgcaacgg ccagctcgac ttcgcctcgc tcggcgccct gatccgcagc ctcgacacgc
     6421 cgccgccgct ggacgagctg atccgcccgc gcgaggccga cgacctcgcc tcgctcaggg
     6481 cgctggcggc gatgcccgac atcgcccgcc tggcgcgcgg ctgggacgcg gtggcgctct
     6541 tgtgggacgt ttgctgcatc cccgacttcc gccagaccat ggccgaggcc catgtccgcc
     6601 tgctcgccga catctatcgc cacctgatgg ggccgtcatc gctgctgccc gcggcctggg
     6661 tccagcgcct ggtcgaccgg ctcgaccaca ccgacggcga catcgacacc cttgccatgc
     6721 gcatcgccca catccgcacc tggaactatg tcggccaccg cccggggtgg gttgccgacc
     6781 ccgcggcgct acaggaccgc gcgcgcgaca tcgaggaccg cctgtcggac gcgctccacg
     6841 accgcctcac ccagcgcttc gtcgaccgcc gcacgaccgt gctgcagcgc cggttgaagc
     6901 agcagcgggc gctggtcgcg gcggtcaagc gctcgggcga ggtggtggtc gagggcgagc
     6961 atgtcgggcg cctcgacggc ttcgacttcg tgcccgacga tgccgccgcg tggtcgcgcg
     7021 cacgcgtgct gcgcgccgcc gtgcgccggg cgctcgagcc cgagatgcgc cggcgcgcgg
     7081 gcgcgctcga ggcggcggcg aacgaggcct tcgcgcttgg ccccgacggc agcctgcgct
     7141 ggcagggcgc caccgtcgcg cggctggtgg cgggcgcggc gctggccgcg ccggggctcg
     7201 cgctcaccgt cggcgacctg gtcgaggagc cgctgcgccg gcgcatcgag aagcgcctcg
     7261 gcgcctggct gcgctggcac gtcggcgagc gcttcgcgcc gctgacccgg gcgcgcgagg
     7321 cgccgctggc ggggccggcg cgcggcctcg ccttccagct tgccgaggcg ctgggctcgg
     7381 tgccgcgggc gaacgtggcc gagcagatcg cggcgctcga tgcgcgcgcg cgcaaggaca
     7441 tcgcccggct cggcatccgg ctcgggcgcg agagcgcgtt catgccggcg atgctcaagc
     7501 cgcggccggt agcgttgcgg gcgctgctgt ggtgcgtggc cgagcggatg cggccggtcg
     7561 cgccgccggc cgggcaggtg tcgctggccc tcgacccggc cttgcccgag cgcttctacg
     7621 ccgccatcgg ctatcgggtg ctcggcgcgc gcgcgctcag ggtcgacatg gtcgagcggc
     7681 tggcgggcga gctcaggcgg cgtgcgcgca ccggcccggt gacgcccgac gccgcgctgc
     7741 tcaaccttgc cggctgcgcc ggcgagggct tcgccggggt ggtcgccggg ctcggcttca
     7801 aggctctgcc cgttgccgcg gacggcgccg tggcttacgt gcccatgcgc gcgccgcgcc
     7861 gcgaggccgc cgggtcgcgg ccgcggccgc cgcggcggcg cgggccggac ggcgagtcgc
     7921 cgttcgccgc gctcgccgcg ctcaagcgcc gccgacggtg agcgccgccg gacccgccgc
     7981 cgccgccgcg gcggagacgg ggatgcgtct ggaccgctgg ctgtggtgcg cccgcttctt
     8041 caagtcgcgc acccaggccg ccaagctgtg cgccggcagc cgggtgcgcc gcaaccggcg
     8101 cgtgctgacc aaggccagcg ccacggtgcg ggtgggcgac gtgctgacct ttccccaggg
     8161 cgcgcgggtg cgcgtggtcc gcgtccttgc gctgcccggc cggcgcggcc cggcggcgtc
     8221 ggcgctggcc ctgttcgacg acctgtcgcc gccgcctgcg ccccagcccg gcgggcggcc
     8281 ggccggcagc ggccggccga ccaaggccga ccggcgcgca ctcgagcacc tgcgcgacgc
     8341 cgagccctag gcgtcgcgcc ccgatgccgc cggcggtgcg gttgccggcg cgcggagggc
     8401 tgtggtaccc agcggcgatg atcgagcgaa tcgcgggcct gctgcgccgg ctggaggcgg
     8461 gtccgcaggt gccgcattcg gccgccgaca tggagcttgc ggcgtgcgcg ctgctggtcg
     8521 aggcggcgtg catggacggc accttcgcgc ccgaggagcg ggccaccatc gagcgcctga
     8581 tcggcgcccg cttcgggctc gacgccggtg ccgccgcggg cctcgtgggc gaggccgagg
     8641 gcgtggtcgc ggaggcgccc gacctgttca ggttcaccaa cgccatcaag tcgagcttca
     8701 gcgaggccga gcgcatcgag ctgatcgaaa tgctgtggga ggtggcctac gccgacggca
     8761 ccgtccacgc ctatgaagac aatctgttgc gccggatcgc cggactgatc tatgtcacgg
     8821 atcgcgaccg cgggctcgcg cgcgcgcgcg tcctggagcg gctggggcga tcgtgataga
     8881 gcagggccaa cgatcgggtg cgccggcccg ccgggacgcg cccgacaggc gagcgccggg
     8941 gcgagcgagg cccgccgtgg ggaaggagac ggacctatga cctatgtcgt gaccgaggcg
     9001 tgcataaagt gcaaatacat ggactgcgtc gaggtctgcc cggtcgattg cttctatgaa
     9061 ggcgagaaca tgctcgtcat ccatcccgac gagtgcatcg actgcggcgt ttgcgagccc
     9121 gagtgcccgg ccgaggcgat cctgcccgac agcgagggcg agctcgacaa gtggctggcg
     9181 ctaaacagcg aatacgccga caagtggccc aacatcaccc gcaagggccc ggccccggcc
     9241 gacgccgacg acttcaaggg cgtgcccggc aagttcgacc agtacttcag cgccaatccc
     9301 ggcaagggca gcggctagcg ccggcccggc accgccaagg cgcccccggc cggcgacaaa
     9361 tcggggcttt gcctggactt cgcggtttct tttgccggga atttgtgata gtttggtttg
     9421 cgctacgtga acgattgaga tcatcacgcg ccgccccgcg gggcggccgt gtgatttctg
     9481 gtagcgccgg atccggaccg ccccgtgcca gggagggctc ggagcaaccg gtgtcgcgct
     9541 ccgccgcgtg cctgctcggc ccggcctcgc accgcgtgcg cgtgcggcgg ccaccgatcg
     9601 ggtgccccgg cggcggccgg cagcgggcaa cgatgcaacg cgggagtcgt gccggcgggc
     9661 actggtgcca gtggccgcga ggggggcatg gacgacaaca acgactgccg ggaccaatca
     9721 gaacaaggac ttgtgttggg gcgaagccaa atggcgaaga aactggagta ccggccgggc
     9781 gacctggtgg tctatccctg ccatggcctg ggtgagatcg tcggtgtcga gacctctgag
     9841 ttcggcggcg tcgagctgcg gctgtacgtg atctcgttcg agaaggacag gatgatcctg
     9901 cgggtgccgg tcgataaggc gtccgggtcc ggcgtgcggc gggtgagcac gcgcgacgag
     9961 atgcagaagg ccttgaccac gctgcgcggg cgggcgcggg tcaagcgcac catgtggagc
    10021 cgccgcgccc aggagtacga ggccaagctc aactcgggca acccgatctc gatcgccgag
    10081 gtggtgcgcg acctgcaccg ggccgccgac cagcccgagc agtcctacag cgagcgccag
    10141 ctctacgagg cggccctcga ccggctgtcg cgcgagtacg ccgcggtcga gaacatcgag
    10201 cagtcggtcg cgaccaagcg gctcgaggac gtgctcaaga aggcggcctg agcgtcgcat
    10261 cgccgacagg ctcgccgccc ggctgaggcg agccagggag accgggcgcg tggccctcga
    10321 cgatcgcgtc aagttcggca ccgtggaacg cgcccggcgc atctgggcgg tgggtgcggt
    10381 ccacggcgag ttcgaccgcc tgtccgccct ccaccagcgc ctcgcgcgcg gctttcgcgc
    10441 cggcgaccgc ctggtctatc tcggcaacta tctcggccgc ggcgcgcgca tcgtcgacac
    10501 catcgacgag atgctcctgt tccggcgcgc gctgatggcg cgcttttccc tgttcgagac
    10561 cgacgtccag ttcctgcgcg gcagccagga ggagatgtgg cagaagctgc tccagctgca
    10621 gctcgcccag tcgccggcgc aggtgttcga gtggatgatc acccagggcg tcgaggcgac
    10681 cttgcgcgcc tacggcgtca accccgacag cggccgcatg cgctgccgcg acgggccgct
    10741 ggcgctggcg cgctggtcgc tggagctgcg cgaggcgatc cgcgcgcacg ccggccacga
    10801 gcagctgctg tcggcgctgc gccgggcggc gtacaccgac gacggccaca tcctgttcgt
    10861 ccatgccggc atcgacacca gccggccgct cgccgcccag accgacagct tctggtgggg
    10921 cggcggcgac ttcgccggca tcgtcgagcc ctatgagggc ttcagccgca tcgttcgcgg
    10981 cttcgatccc ggccatggcg gcgtcgacat cggcgtggtc gcggcgaccg tcgattcggg
    11041 ctgcggcttc ggcgggccgc tgaccgcgat ctgtctcgag cgcaccggcg aggtggtcga
    11101 ggcgatcgag ggctgatcgc cgccgcccgc ccccgcctgt gcgggcgccg gccattccgg
    11161 catcatcaca caaatcatta gctgaccaac catctccgcc gccggcgccg cacacgcggc
    11221 ctccgccgcg caggccgcgt cgatccgggc agggatttcc ccggccggaa ttatttcgat
    11281 cgccgccgac gggcggcgac gggcgtcgcg gcgaggcccg ggcgcgcggt gagtgggtca
    11341 tttcgccgcc atcggaccgc gttcgcggcg gctcgcgacc cggatgtcgc gctcgctctg
    11401 cgattttttc aaagcccatc ggccgctgcc ggcggccgtc cgaggcggtg ctctcgccct
    11461 cgtcggcgca ccgaatcgat gccgccggtg ccgtttcgcg gccaaaatgg cacgcggatt
    11521 gcaaagacca tttcgcgacc gtgccggctg cggtcgccga atggagtcga atttggataa
    11581 aattgtcgga aaatttccct aagttatcaa ataaaacccg agggaatttt gacaaatcgc
    11641 gaagtgaaaa aattttttac cctatggact ctagggaaag cactgtgttt aattttgcgg
    11701 cttgcgtaac gaggcgtgag ctctgcttgt tagccggcag aagagccaac gactaagaag
    11761 caatgaaagg tgcgaccgaa ccatggccta tgtagataca ccgcagacgc agaaggcgaa
    11821 tcggcggtac atcaaccggt cgatgaaggc tgccttgctg actcgtgaac gcgagttcga
    11881 gctggcgcgc gcgtggcgcg agcagggcga cgaacgggcg ctgcacgagt tggtcaacgc
    11941 ctacggccgt ctggtgatca agctcgcctc gcgattccgc aattacggcc tgccgatgag
    12001 cgatctggtg caggagggca atatcggact gttgcaggcg gccgcgcgct tcgagccgga
    12061 gcgcgaggtg cgtttctcga cctacgccgc gtggtggatc cgctcggcga tgcaggactt
    12121 cgtgctgcgc aactggtcga tcgtgcgcac cggcacgacc gctgcccaga aatcgctgtt
    12181 cttcaatttg cgccggctgc gcgccaagca tgccgacgac gtcgacggct ggctgagcga
    12241 ggaggcgaag gatcgcatcg ccgacgagct cagggtccgg gtcaacgacg tcgaggacat
    12301 ggagatgcgc ctcgccggca acgaccagtc gctcaacgcc tgcgtcagcg acgcgagcga
    12361 ggacgagtgg caggacttca tgtccgacga ccggccctcg cccgaggacc aggtgcgcga
    12421 catgcacgac agcgccaccc gctcgcgctg gctccaggcg gcgctcgacg agctcggcga
    12481 gcgcgagcgc acgatcatcc acgagcgccg gctgcgcgac gacccggtca ccctcgagga
    12541 gctcggcaag gcgctcggca tcagcaagga gcgggtgcgc cagatcgagc acaaggcgct
    12601 ccagaagctg cgcgcgtcga tcacgcgcca gagcgacgag gcacggcgca cgacggtggc
    12661 ctgagtcccc gccgcccgcg cgtcacgagg acgggccgcg gccgcgggcc gcggcccgac
    12721 tcgtctggcg gccgtcggcg agcagctcga tctgctcggg atgggtggcc tcgatctcgg
    12781 gcccgttgta gcggtcgatc cagccgcgca ccctgacccg gcggccggcc agcgcctcga
    12841 gcgagtcgag gcccaaggcg gcgaaggcgc gctcgaagtc cgggcgcagg cgcggcgcga
    12901 ccgtgaccgt gaagtcctcg cgccagtcgg cgccgaagtt gaggtacagc cgcccgcgca
    12961 ccagcgcggc cgcctcgacc gtaccctcga ccagctggaa gctgccgatc gcgccgtcga
    13021 gccgatccgc ggcgcgcggc cgatagaacg ggtgcgccca gatgccgcgc agcgccgccc
    13081 gtgccccggc ctcgctcgcc agcatgtcgg caaccagcgc ccggttgtcg gcgaagctgt
    13141 agacccgtgc catgccgcgg gccagcatcc ggtcctggat ccagacgccg tcggcgcgca
    13201 ccagatgggc gagcgcgcgc ccgtggcggt cgctgcgccg gccgccgtag cgcaatgtca
    13261 cccgctgccc gagggcgagc tcggcgagcg ccgcacgcgc ctcctcggcc agcggccagt
    13321 cgctcaagcc cgcacgcccg agcgcgagct tgggcgcctg caggccgacc aggcgcacca
    13381 cggtgccgtc ggcaagcgcg agggtgtcgc cgtcgaccac cagggcgacc acgccctcgc
    13441 cgcccggctc gagcgcgtcc gccggcgtcg ccgcgtgcac cggcgcggcg gcggccacca
    13501 aggccgcgac gacggcggca aggcataggc gcgcgggcgc aggaaaccgg cccaaacgat
    13561 ccgatgctgg tatcatggcc gcgccttcga gacggtgagc ggcgacgtgc gcgaccttta
    13621 tcccgacatc gaaccattcg caaccggacg cctgccgctc gagccgccgc acgtcatgta
    13681 ctgggagcag agcggcgccg aggacggcgt gccggtgctg ttcctgcacg gcggccccgg
    13741 cgccggcgcg accgcggcca accggcgctt cttcgactcg gcccattacc ggatcatcgt
    13801 ctacgaccag cgcggcgccg gccgctccga gccgctcggc gagctcgccg ccaacaccac
    13861 gcccaacctg gtcgaggaca tcgaggcgct gcgcgaccat ctcggcatcg gcagctggct
    13921 ggtgttcggc ggctcgtggg gctcgacgct cgcgctcgcc tacggcgaaa gccatcccga
    13981 gcgctgcctg gcgctggtcc tgcgcggcat cttcctgtgc cggcgggcgg agatcgactg
    14041 gttcgtcaac ggcatccggc gattcttccc cgagccctgg caagccttcg ccggccatct
    14101 gccggaggcc gagcagggcg acctgctggc cgcctaccac cggctgctca ccgaccccga
    14161 cccggcggta cacatgcccg cggcgcgcgc ctggagccgc tacgaagggt cgtgctcgac
    14221 gctccagccg agccccgaga cggtggccga gttcggccgc gaccggatgg cgctcggact
    14281 ggcgcggatc gaggcccatt acttcctcaa cgacatcttc atgccggcca accatttgct
    14341 cgaaaatgtc gggcggctga ggtcgatacc cggctatatc gtccagggac gttatgacat
    14401 ggtgtgcccg atcgagaccg cgtgcgacct ggtgcgggcg tggcccgaag cggagctcca
    14461 cgtggtccag gacgccggcc actcgtcgat ggagccgggc atccgcgcca agctggtcga
    14521 gatcatggac cgcctgcgcg accacg
//
LOCUS       DASWHU010000137         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_427924, whole genome shotgun sequence.
ACCESSION   DASWHU010000137 DASWHU010000000
VERSION     DASWHU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427924"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGB88_05030"
     CDS             <1..183
                     /locus_tag="VGB88_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9646839.1"
                     /translation="VRADGSLIAGEVSGSIHSVAAAIQGAPACNGWAFWFFEAKGKLV
                     PIDLLRQQVRAELAHA"
     gene            224..523
                     /locus_tag="VGB88_05035"
     CDS             224..523
                     /locus_tag="VGB88_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646840.1"
                     /translation="MNGQDMVGDDVAAAATEIVEREMGPFGLRAVTVTAAEDHDGDPI
                     LLVEVEYRAGGKPIEPKVVAGLATKLRRRLWEMGEMRFPHVRHHFSARQKVARYP"
     gene            520..981
                     /locus_tag="VGB88_05040"
     CDS             520..981
                     /locus_tag="VGB88_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646841.1"
                     /translation="MTDDLVATARRLAKASRGKPRQADLKRAISTAYFALFHALARDT
                     ADLLVGVGPSRPDKAWAQAYRALGHGFAKNACGQVRALGFPPAVCACADAFVTLQQAR
                     HDADYDPRHRVSRADAMAAIALAEQAIRDLRTAPRKYRLAFAIQLHFPKRT"
     gene            complement(1109..2194)
                     /gene="mutY"
                     /locus_tag="VGB88_05045"
     CDS             complement(1109..2194)
                     /gene="mutY"
                     /locus_tag="VGB88_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX9646842.1"
                     /translation="MAAARTSASGAAAELLAWYDRNRRSLPWRAAPGARADPYRVWLS
                     EVMLQQTTVAAVIPYFERFMAEWPTVEALAAAPRESVLAAWAGLGYYARARNLHACAQ
                     AVVARHGGAFPAEERALRALPGIGPYTAAAITAIAHQRPAAVVDGNVERVMARAFAVA
                     TPLPAAKPELRRLAQSLTPEACPGDYAQAVMDLGATVCTPTSPRCGACPWSARCLARA
                     QGIADQLPRRVAQRARPTRRGIAFWIERADGAVLLRRRPDSGLLGGMVELPSTEWGEA
                     APAPEDAAAGAPVAIRDWRLLPGTVRHTFTHFHLELAVLAAHAAEPAPAGLWCAHESL
                     HRQGLPTVMRKLAAHALSARRTRSAGS"
     gene            2265..2759
                     /locus_tag="VGB88_05050"
     CDS             2265..2759
                     /locus_tag="VGB88_05050"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX9646843.1"
                     /translation="MAVTRTRKGGPRAAGALVPALTRTAFAARGLHEAAVITDWPAIV
                     GEALARYSAPERLKGDGTLVVRVAGGAAALELQHLAPVVLARIATYFGFRAALRLALR
                     QGPLPPRPAPPTAPRTGDVPLAEPARSAVDEVAEPGLRAALARLGGALAAESRAREEG
                     DGGG"
     gene            2822..3484
                     /locus_tag="VGB88_05055"
     CDS             2822..3484
                     /locus_tag="VGB88_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9646844.1"
                     /translation="MRRAALAIVLACGLGAATAVAEEPAAVSAAPKSLGDPAAPVKII
                     EYASLSCPHCAEFHAERMPWLKQTYIDTGRVRFEFRDFPLNAPALWGSMLAHCAGPDR
                     YYAWLDLLFGRQRDWAVGSTAIGGGNADAFFESGCPKQDWPEVWHRLAGVGKLAGMGE
                     ARLQSCLCDQDLADAIIRTRSDGQKEFDIESTPSLVIDGEVFPGVPSEDELSKRIEDA
                     GG"
     gene            3619..>3927
                     /locus_tag="VGB88_05060"
     CDS             3619..>3927
                     /locus_tag="VGB88_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9646845.1"
                     /translation="MQFTRLKLSGFKSFVDPTELLIGSGLTGIVGPNGCGKSNITDAV
                     LWALGEQSPLAVRGQTMQDMIFAGGEQSSPRRHAEVEVVLDNSDGAAAGSEFSEISVK
                     R"
BASE COUNT          530 a         1399 c         1439 g          559 t
ORIGIN      
        1 gtgcgcgccg acggcagcct gatcgcgggc gaggtgtcgg gctcgatcca cagcgtcgcc
       61 gccgccatcc agggcgcgcc ggcgtgcaac ggctgggcgt tctggttctt cgaggccaag
      121 ggcaagctgg tgccgatcga cctgctgcgc caacaagtcc gcgccgagct cgcccacgcc
      181 tgagccgctg gccttcacgg aatggcgttg ataagctttg gtcatgaatg gccaggatat
      241 ggtcggggac gatgttgccg ccgccgccac ggaaatcgtc gagcgggaaa tggggccgtt
      301 cgggctgcgc gccgtcacgg tgacggcagc tgaggatcac gacggcgacc cgatcctcct
      361 ggtcgaggtc gaataccgcg ccggcggcaa gccgatcgag cccaaagtgg tcgccgggct
      421 cgccaccaag ctccggcgtc ggctgtggga gatgggggaa atgcgcttcc cgcatgttcg
      481 tcatcacttc tccgcccgtc agaaggtggc ccgctatccg tgaccgacga cctcgtcgcc
      541 acggcaaggc gtctcgcgaa agcaagccgt ggcaagccca ggcaagccga cctcaagcga
      601 gccatcagca ctgcctactt cgccctcttc cacgcgttgg cgcgggacac cgccgatctg
      661 ctcgtcggcg tcggcccgag ccggcccgac aaggcgtggg cgcaggcata ccgcgctctt
      721 ggtcacggat tcgcgaagaa tgcatgcggg caggtgcggg cgctgggctt tccgccggcg
      781 gtatgcgcgt gcgccgatgc gttcgtcacc ttgcagcagg ctcgccacga tgccgactat
      841 gatccccgcc acagggtatc gcgcgccgac gcaatggcgg cgatcgcgtt ggcggagcag
      901 gccatccgcg acttgaggac ggcgccgcga aagtatcggc tggctttcgc cattcagctt
      961 catttcccga agcggacctg agtcggacct tcgattcggg caaccgttcg tgagtcccga
     1021 agtatcggcg accggtcagg actggcagtc cacttcggct tggaatccgg catcggccgt
     1081 cgccgtccat ccggctctgt ccgcacgact atgacccggc cgagcgagtg cgccgcgcgc
     1141 tgagcgcgtg ggcggcgagc ttgcgcatga ccgtcggcag gccctggcgg tggagagact
     1201 cgtgcgcgca ccacaggccc gcgggtgcgg gctcggcggc gtgggcggcg agcacggcga
     1261 gctcgaggtg gaagtgggtg aaggtgtggc gcaccgtgcc cggcaacagg cgccagtcgc
     1321 ggatcgccac cggcgccccc gcggcggcgt cctcgggcgc cggcgcggcc tcgccccact
     1381 cggtcgaggg cagctcgacc atgccgccga gcaggcccga gtcgggccgg cgccggagca
     1441 gcaccgcgcc gtcggcgcgc tcgatccaga acgcgatgcc gcgccgggtc gggcgcgcgc
     1501 gttgcgctac gcgccgcggt aactggtcgg cgatgccttg tgcgcgcgcc aagcaccgcg
     1561 ccgaccacgg gcaggcgccg cagcgcggtg acgtgggcgt gcacaccgtc gcgcccaggt
     1621 ccatcaccgc ctgggcgtag tcgcctgggc acgcttcggg cgtgagcgat tgggcgaggc
     1681 gcctgagctc gggcttggcc gcgggcagcg gcgtcgcgac cgcaaatgcg cgcgccatca
     1741 cccgctcgac attgccgtcg accaccgccg ccggccgttg atgcgcgatc gcggtgatcg
     1801 ccgccgcggt gtagggcccg atgccgggca gcgcgcgcaa ggcgcgctcc tcagccggga
     1861 aggcgccgcc gtggcgcgcc accacggctt gcgcgcaggc gtgcaggttg cgtgcgcggg
     1921 cgtagtagcc gaggcccgcc caggcggcga gcaccgattc gcgcggcgcc gcggcgagcg
     1981 cctcgaccgt gggccattcg gccatgaaac gctcgaaata ggggatcacc gcggcgaccg
     2041 tggtctgctg cagcatcacc tcgctcagcc acacccggta gggatcggcg cgcgcccccg
     2101 gcgcggcccg ccacggcaag ctgcggcggt tgcggtcgta ccaggcgagc agctccgccg
     2161 ccgcgccgct tgcgcttgtc ctcgcagccg ccatcgagtg cctgtaccct tggtgcgtgc
     2221 ggtgacacta gcgcgcggcc ggtgccgcgc aagggagagc gcgggtggca gtgacgcgga
     2281 cccgcaaggg cgggccgaga gcggccgggg cgctggtgcc ggcgctgacc cggaccgcgt
     2341 tcgccgcgcg cgggctgcac gaggcggcgg tgatcaccga ctggccggcg atcgtcggcg
     2401 aggcgctcgc ccgctactcg gcgcccgagc ggctcaaggg cgacggcacg ctggtggtcc
     2461 gggtcgcggg cggggcggcg gcgctcgagc ttcagcacct ggcgccggtg gtgctggcgc
     2521 gaatcgcgac ctatttcggc ttccgcgccg cactgcggct ggcgctgcgc caggggccgc
     2581 tgccgccgcg gccggcgccg cccacggccc cgcgtaccgg cgatgtgccg ctcgccgagc
     2641 ccgcgcggag cgcggtggac gaggtcgccg agcccggctt gcgcgccgct ctcgcccgcc
     2701 tcggcggcgc cctcgccgcc gaatcacgag cccgcgagga gggcgacggc ggcggctaag
     2761 gcgccttgtc ccgccggccg gcccggccta tctatggccc cagcaacacg gaggtccgcc
     2821 gatgcgccgc gccgcccttg ccatcgtcct tgcctgcggg ctgggcgccg ccacggccgt
     2881 agccgaggag cccgccgcgg tctcggcggc gccgaagtcg ctcggcgatc cggcggcgcc
     2941 ggtgaagatc atcgaatatg cctcgctcag ctgcccgcat tgcgccgagt tccatgccga
     3001 gcggatgccc tggctcaaac agacctacat cgacaccggc cgggtccggt tcgagttccg
     3061 cgacttcccg ctcaatgcgc cggcgctgtg ggggtcgatg ctcgcccact gcgcgggccc
     3121 cgaccgctac tacgcgtggc tcgacctgtt gttcgggcgc cagcgcgatt gggcggtcgg
     3181 cagcaccgcg atcggcggcg gtaacgccga cgcgttcttc gagtccggct gccccaagca
     3241 ggactggccc gaggtgtggc accggctcgc cggcgtcggc aagcttgccg gcatgggcga
     3301 ggcccggctg cagtcgtgcc tctgcgacca ggacctcgcc gacgcaatca ttcgcacgcg
     3361 cagcgacggc cagaaggagt tcgatatcga atcgacgccg agcctggtca tcgacggcga
     3421 ggtgttcccg ggcgtgccga gcgaggacga gctgagcaag cggatcgagg acgcgggcgg
     3481 ctgagcgccc ccggcgcgcg cgccgcgccc ggcacgcgat tgccttgttg tgacgccgcc
     3541 ggccgattcc tataatcggt cgtcccgcgg ccgttgaccc aaggcgtcga tcgcttcccc
     3601 tctgaccggg cgcacgcatt gcagttcacc cgcctgaagc tttccggctt caagtccttc
     3661 gtcgatccga ccgagctcct gatcggttcc gggctgaccg ggatcgtcgg ccccaacggc
     3721 tgcggcaagt cgaacatcac ggacgccgtg ctgtgggcgc tcggggagca gagtccgctt
     3781 gcggttcgcg gccagacgat gcaggacatg atcttcgccg gcggcgagca gtcctcgccg
     3841 cgccgccacg ccgaggtcga ggtcgtgctg gacaactcgg atggcgcggc ggccggcagc
     3901 gagttctcgg agatctctgt caagcgg
//
LOCUS       DASWHU010000138         5551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3026253, whole genome shotgun sequence.
ACCESSION   DASWHU010000138 DASWHU010000000
VERSION     DASWHU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026253"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VGB88_05065"
     CDS             complement(<1..150)
                     /locus_tag="VGB88_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEX9646846.1"
                     /translation="MTGDVNKVVLAYSGGLDTSVILRWLQETYACEVVTFTADLGQGE
                     ELGPAR"
     gene            complement(223..462)
                     /locus_tag="VGB88_05070"
     CDS             complement(223..462)
                     /locus_tag="VGB88_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646847.1"
                     /translation="MDVLDRLDEQVLDAVPLAVLGAVLVLVLWLCARILRRAGIPGWL
                     ALLVLIPGWNLVAVWALAFLPWPAELRRGRPGRRD"
     gene            complement(468..884)
                     /locus_tag="VGB88_05075"
     CDS             complement(468..884)
                     /locus_tag="VGB88_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646848.1"
                     /translation="MNWAAMAEDIAIAYGSPLVVAAVLAIPFWRMLGRVGLNPYWTVL
                     LVAPVIGVVMVLAILAFGPWPLVEGRATIARRPARGRPARATALPAPARPWGEPPRPA
                     PNAAAPQPSAPQPARATGVPRHSWRRAGTVVRRRRD"
     gene            complement(925..1299)
                     /locus_tag="VGB88_05080"
     CDS             complement(925..1299)
                     /locus_tag="VGB88_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646849.1"
                     /translation="MSGSDGPLGLRAAGPEDLAQLEAWCHALGGERPPFVSVTLSTFL
                     RAPERGELLIVVDGGAERGFVVLARLWSNRLRAEVAVIDDWLLEPGVDEELALFEVSR
                     HARALGAAEVAVRGADGSLGAR"
     gene            complement(1296..2402)
                     /gene="rlmN"
                     /locus_tag="VGB88_05085"
     CDS             complement(1296..2402)
                     /gene="rlmN"
                     /locus_tag="VGB88_05085"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9646850.1"
                     /translation="MTAAPRESRRDLVGLSREEMAAALAEFGAKPFRARQLWHWIYFR
                     GARDFADMTNIPKGLGAELAAAFRIARPAVSALQHSADGTIKWLARLDDGREVECVHI
                     PEDDRGALCVSSQVGCTLSCSFCHTGTQRLVRNLTAGEIVGQMLIARDALGEWPAPRA
                     GRMISNVVMMGMGEPLYNYDNVAAALRIFMDSGGISLSRRRITLSTSGVVPMIARVGR
                     ELGVNLAVSLHAVRDSLRDELVPINRKWPIAELLDACRSYPRASNARRITFEYVMLKG
                     VNDSDSDARELVRLIAGIPAKVNLIPFNPWPGAPYECSSNNRIHAFAEIVNAAGYSSP
                     VRTPRGRDIMAACGQLRSASERARRAPAGAQPVA"
     gene            complement(2399..2911)
                     /locus_tag="VGB88_05090"
     CDS             complement(2399..2911)
                     /locus_tag="VGB88_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX9646851.1"
                     /translation="MIARTVPFAFACLALLLPAARAAEGDEILGQFRDWYAVAYDEQG
                     TKICYMVSRPTNSEGNYDQRGAVYVQVTRRSAEPAPDVVSFEAGYAFLDGAKIEVAID
                     GKNHDLFTKGQTAWAYDADGDKTLVEAMAKGTRMVVKGVSARGTDTTDTYSLLGFTAA
                     QRAMAEACGK"
     gene            3136..4113
                     /locus_tag="VGB88_05095"
     CDS             3136..4113
                     /locus_tag="VGB88_05095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX9646852.1"
                     /translation="MRAVVCKQLGGPEALAVEEVRAPALGPGEVRIAVHAAGLNFADT
                     LIIQGKYQERPEPPFTPGMELAGVVAECAGDVAGLAPGTRVLAMCGTGAFAEEAVVAA
                     DRVVAIPDSMTMVQAAGFPVAYGTSHLALDHRGRLQAGEVLLVLGAAGGVGLTAVEIG
                     KAMGAVVIAAASSADKLAVARERGADHLIDYTAEDLRARVKDIAGGADVIFDPVGGDL
                     FQAALRCINWEGRMLVIGFASGTIPQIPANYLLVKNAAAVGVFWGAYRKNDPARMMGS
                     LDTLLGWFAAGRLRPHISHELPLESAADAFALMIGRKSTGKVVLTTGRA"
     gene            4274..5533
                     /locus_tag="VGB88_05100"
     CDS             4274..5533
                     /locus_tag="VGB88_05100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9646853.1"
                     /translation="MTRTRQAQHTDARVRAQYEDYPYPARKPEDEARRLVTGSPSHLA
                     EIIHYLYAGRAPYGPGKRFRALIAGGGTGDGAIMLAQQLADAGADAEVVHLDVARAAA
                     RIAKARARARGLSNIRFVDGSLLDLEALGLGGFDYIDCCGVLHHLADPAAGLRALVRA
                     LGPGGGIGLMVYGTLGRTGVYHVQDALRLLADAGAPAKDRVALARSLLADLPETNWLR
                     RNPFVADHLHGDDAGLHDLLLHARDRAYTVPEVFALCAGAGLAITSFIEAARYEPATY
                     LHDADLVARAGALAWPERAALAELLSGAIKRHVVYAVTGSAVGRVAAPDSPDMVPVLR
                     EGDGQAYARALAATGRLTAQLENLTLSFSFGPLAPAILSRIDGVKSLAEIHAALTTDT
                     DWAAFKAAFDDLHAKLNGINLLLLRAR"
BASE COUNT          764 a         2020 c         2002 g          765 t
ORIGIN      
        1 ccgcgccggg ccgagctcct cgccctggcc gaggtcggcg gtgaacgtga ccacctcgca
       61 ggcgtacgtc tcctgcagcc agcgcaggat caccgaggtg tcgagcccgc ccgaataggc
      121 gagcacgacc ttgttcacgt ctccggtcat ggtgcactcc acgcatcgaa aagcgccccg
      181 cgaatagccg ggggccggtg cgctgtcaac gagaccgggg gctcagtcgc gccggcccgg
      241 gcggccgcgc ctgagctcgg ccggccaggg caggaacgcg agcgcccaca ccgcgaccag
      301 gttccagccc gggatcagca ccagcagggc gagccagccg gggatgccgg cccggcgcag
      361 gatgcgcgcg cacagccaca gcacgaggac cagcaccgcg ccaaggaccg cgagcggcac
      421 cgcatcgagc acttgttcgt caagccggtc gaggacgtcc atcgcactca atcgcgccgc
      481 cggcgcacca ccgtgccggc gcgccgccac gagtgccggg gcacgccggt ggcgcgcgcg
      541 ggctgaggcg cggacggctg cggtgccgcc gcgttgggcg cggggcgcgg cggctcgccc
      601 cacggccgcg ccggcgccgg cagtgccgtg gcccgcgccg gccgccctcg ggcgggcctg
      661 cgcgctatcg tcgcccgtcc ctcgaccagc ggccacggcc cgaaggcgag gatcgccagc
      721 accatcacca ccccgatcac cggcgcgacc agcagcaccg tccagtacgg gttgaggccg
      781 acccgcccga gcatccgcca gaacgggatc gcgagcaccg ccgcgaccac cagcggcgag
      841 ccgtaggcga tggcgatgtc ctcggccatg gccgcccagt tcacggcaat ccttgccgct
      901 gtcaaggaca accgccgggc cggctcaccg cgcgccgagg ctgccgtccg cgccgcgcac
      961 cgccacctct gccgcgccga gcgcgcgggc gtggcgcgaa acctcgaaca gcgccagctc
     1021 ctcatcgacg ccgggctcga gcagccagtc gtcgatcacc gcgacctcgg cgcgcaggcg
     1081 gttcgaccac agccgcgcga gcaccacgaa gccgcgctcg gcgccgccgt cgaccacgat
     1141 caggagctcg ccgcgctcgg gcgcgcgcag gaacgtgctc aaggtgaccg agacaaaggg
     1201 cgggcgctcg cccccgagcg cgtggcacca ggcctcgagc tgcgccaggt cctcgggccc
     1261 cgccgcgcgc aagccgagcg ggccgtcgct cccgctcaag cgaccggctg agcgccggcg
     1321 ggggcgcgcc gcgcgcgctc gctcgcgctt cggagctggc cgcacgcggc catgatgtcg
     1381 cgcccgcgcg gcgtgcgcac cggcgatgag tagccggcgg cgttgacgat ctcggcgaag
     1441 gcgtggatgc gattgttgct cgagcactcg tagggcgcgc cgggccacgg gttgaacggg
     1501 atcaggttga ccttggccgg gatgccggcg atcagccgca ccagctcgcg cgcgtccgag
     1561 tccgagtcgt tgacgccctt gagcatgacg tactcgaagg tgatgcggcg cgcgttgctc
     1621 gcccgcgggt aactgcggca cgcgtcgagc agctcggcga tcggccattt gcggttgatc
     1681 ggcaccagct cgtcgcgcaa ggagtcgcgt accgcgtgca gcgacacggc gaggttgacg
     1741 ccgagctcgc gcccgacccg cgcgatcatc ggcaccacgc ccgaggtcga caaggtgatg
     1801 cgccgcctgg acaatgagat tccgcccgag tccatgaaga tgcgcagcgc cgcggcgacg
     1861 ttgtcgtagt tgtagagcgg ctcgcccatg cccatcatga ccacgttcga gatcatccgc
     1921 cccgcgcgcg gcgccggcca ctcgccgagt gcgtcgcgcg cgatcagcat ctggccgacg
     1981 atctcgcccg cggtcaggtt gcgcaccagc ctctgggtgc cggtgtggca gaacgagcag
     2041 cttaaggtgc agccgacctg cgaggagacg cacagcgcgc cgcggtcgtc ctcggggatg
     2101 tgcacgcact cgacctcgcg gccgtcgtcg agccgggcga gccacttgat ggtgccgtcg
     2161 gcggagtgct gtagcgcgct caccgccggc cgggcgatgc ggaacgcggc ggcgagctcg
     2221 gcgccgagcc ccttggggat gttggtcatg tcggcgaagt cgcgcgcgcc gcggaaatag
     2281 atccagtgcc acagctggcg ggcgcggaac ggcttggcgc cgaactcggc cagcgccgcc
     2341 gccatctcct cgcggctgag cccgaccagg tcgcgccggc tctcgcgtgg cgccgcggtc
     2401 atttgccgca cgcctcggcc atcgcccgct gggcggcggt gaagcccagc agcgagtagg
     2461 tgtcggtggt gtcggtgccg cgcgcggaga cccccttgac caccatcctg gtgcccttgg
     2521 ccatggcctc gaccagggtc ttgtcgccgt cggcgtcgta ggcccaggcg gtctgtccct
     2581 tggtgaacag gtcgtggttc ttgccgtcga tggccacctc gatcttggct ccgtcgagga
     2641 aggcgtagcc ggcctcgaag ctgaccacgt cgggggcggg ctcggcgctg cgccgggtga
     2701 cctggacgta gaccgcgccg cgctggtcgt agttgccttc gctgttggtc ggccggctga
     2761 ccatgtagca gatcttggtg ccctgctcgt cataggcgac cgcataccag tcgcggaact
     2821 ggccgaggat ctcgtcgcct tcggccgcgc gcgcggccgg cagcaggagc gccagacaag
     2881 cgaaagcaaa cgggactgtt cgggcaatca ttgcgcgggc gtctatttcg aggctgcggg
     2941 cacgagccgg cccgacccgc acgggcgcgg cccgggcgga ttgcccatcc aggccggctt
     3001 tgatatcggt tcggcgccga tggcgagtcg acctgtcacc agttcgggca tggatcgggc
     3061 ggtgttcctt cgcatcatcc cggcccgccc gacgcgtggc ggcgggcgcg gccgggccag
     3121 acaggggaga taaccgtgcg cgcggtggtt tgcaagcagc tgggcgggcc cgaggcgctg
     3181 gcggtcgagg aggtccgggc gccggcgctc ggccccggcg aggtacgcat cgcggtccat
     3241 gccgccgggc tcaacttcgc cgataccctg atcatccagg ggaagtacca ggagcgcccc
     3301 gagccgccgt tcactccggg catggagctc gccggggtgg tcgccgagtg cgccggcgac
     3361 gtcgccgggc ttgcgccggg cacccgggtg ctggcgatgt gcggcaccgg cgccttcgcc
     3421 gaggaggcgg tggtcgccgc cgaccgggtg gtggcgatcc cggactcgat gaccatggtc
     3481 caggcggcgg gcttcccggt cgcctacggc acctcgcatc tcgccctcga ccaccgcggc
     3541 cggctgcaag ccggcgaggt gctgctggtg ctcggcgccg cgggcggggt cgggctgacc
     3601 gcggtcgaga tcggcaaggc gatgggcgcg gtggtgatcg ccgccgcctc gagtgcggac
     3661 aagctcgcgg tggcgcgcga gcgcggcgcc gatcacctga tcgactacac ggccgaggac
     3721 ctgcgcgccc gggtcaagga catcgccggc ggcgccgacg tgatcttcga tccggtcggc
     3781 ggcgatctgt tccaggcggc gctgcgctgc atcaactggg aggggcggat gctggtgatc
     3841 ggcttcgcca gcggcaccat cccgcagatc ccggccaact acctcttggt caagaacgcc
     3901 gccgcggtcg gcgtgttctg gggcgcgtac cgcaagaacg acccggcgcg catgatgggc
     3961 tcgctggata cgctgctcgg ctggttcgcc gccggccggt tgaggccgca catctctcac
     4021 gagctgccgc tcgagtccgc ggccgacgcg ttcgccctga tgatcggccg caagtcgacc
     4081 ggcaaggtgg tgctgaccac gggacgcgcg taatttttcc aagctccctg ctcgaagccg
     4141 gacaaatgct tgtgaccgtt ttgccatgtg gcgcgaagga ttcgggcacg cggagccgcg
     4201 gagcggctga gaaacgcgtt ttcgcgtctc cgcgcctccg cgtgcatcca ttcatcgggc
     4261 gcttgcggat cgcatgacca ggacgcggca ggcgcaacac accgacgcgc gggtgcgcgc
     4321 gcagtacgag gactacccct accccgcgcg caagcccgag gacgaggcgc ggcgcctggt
     4381 caccggctcg ccgagccacc tcgccgagat catccactac ctgtacgccg ggcgcgcgcc
     4441 ctacgggccg ggcaagcgct tccgcgcgct gatcgcgggc ggcggcaccg gcgacggggc
     4501 gatcatgctc gcccagcagc tcgccgacgc cggcgccgac gccgaggtgg tgcatctcga
     4561 cgtcgcgcgc gcggccgcac gcatcgcaaa ggcgcgggcg cgcgcgcgcg gtctcagcaa
     4621 catcaggttc gtagacggct cgctgctcga cctcgaggcg ctcggcctcg gcggcttcga
     4681 ctacatcgac tgctgcggcg tcctgcacca tctcgccgat ccggccgcag gcttgcgcgc
     4741 tctggtgcgc gcgctcggcc ccggcggcgg gatcgggctg atggtctacg gcacgctcgg
     4801 gcggaccggc gtctaccacg tccaggacgc gctgcggctg ctcgccgatg ccggcgcgcc
     4861 ggcgaaggac cgggtggcgc tcgcgcgttc cctcctcgcc gatctccccg agaccaactg
     4921 gctcaggcgc aacccgttcg tcgccgatca cctgcatggc gacgatgccg ggctccacga
     4981 cctgctgctg cacgcgcgcg accgcgccta caccgtgccc gaggtgtttg cgctgtgcgc
     5041 aggcgcgggt ctcgccatca cctcgttcat cgaggccgcg cgctacgagc cggcgaccta
     5101 cctgcacgat gcggacctgg tggcgcgcgc gggcgcgctc gcctggcccg agcgcgcggc
     5161 gctcgccgag ctgctgtcgg gggcgatcaa acggcatgtg gtctacgcgg ttaccggcag
     5221 cgccgtgggc cgcgtcgccg cgcccgactc gcccgacatg gtgcctgtgc tgcgcgaggg
     5281 cgacggccaa gcctacgccc gcgcgctggc ggcgacgggg cggctcaccg cgcaactcga
     5341 gaacctgacc ctgagcttct ccttcggccc gctcgcgccc gcgatcctgt cgcgcatcga
     5401 cggcgtcaag agcctcgccg agatccacgc cgcgctgacc accgacaccg actgggcggc
     5461 gttcaaggcc gcgttcgatg accttcacgc caagctcaac ggcatcaacc tgctgctgct
     5521 gcgcgcgcgg tgacttgcag gttgccgagt c
//
LOCUS       DASWHU010000139        13103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3380711, whole genome shotgun sequence.
ACCESSION   DASWHU010000139 DASWHU010000000
VERSION     DASWHU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3380711"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGB88_05105"
     CDS             complement(<1..397)
                     /locus_tag="VGB88_05105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9646854.1"
                     /translation="MASADGSAADPENTLYMDLESGRVVIELRPDLAPKHVARIKALV
                     RQGFYDGLSFHRVIDGFMAQGGDPKGDGTGGSGQRIPAEFSPPSVAKHLRGTVSMARS
                     QHADSADSQFFICFEPAPFLDGKYTIWGQV"
     gene            complement(472..993)
                     /gene="coaD"
                     /locus_tag="VGB88_05110"
     CDS             complement(472..993)
                     /gene="coaD"
                     /locus_tag="VGB88_05110"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9646855.1"
                     /translation="MTDPRTGIYPGTFDPTTVGHLDIIRRALRVVDKVVIGVAVNAGK
                     APMFEIGDRVAMLREAVAAMDHDVASRVSVTAFDNLLIHFASQQGAGVIIRGLRAVSD
                     FEYEFQMAGMNARLAPDIETVFLMASEGIQFIASRLVKEIYLLGGDVTQFVPPNVARR
                     LDDHISRQIGGTG"
     gene            complement(986..3718)
                     /gene="gyrA"
                     /locus_tag="VGB88_05115"
     CDS             complement(986..3718)
                     /gene="gyrA"
                     /locus_tag="VGB88_05115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9646856.1"
                     /translation="MTDSAQAQPFDISPVSIEEEMKRSYLDYAMSVIVARALPDVRDG
                     LKPVHRRILFSMHENNYDWSHPYRKSARVVGDVMGKYHPHGGEAIYDAMVRMAQEFSM
                     RLPLIDGQGNFGSMDGDRAAAMRYTEARLERAAGALLEDLDKDTVDFQPNYDSTENEP
                     GVLPARFPNLLVNGAGGIAVGMATNIPPHNLREVVAACLALIDDPALSIEALMEHIPG
                     PDFPTGGIIIGRAGIRAAYHTGRGAVVVRGRVMSERDDRVIVITEIPYLVNKSRLVEQ
                     IAEAVRERRIEGVSDLRDESDRDGVRVVVELKRDASYDVVLSQIYRYTPLQTSFGVNA
                     LALVDGRPEQLDLKRMLQAFLRFRERVVTRRTKFELARARERAHILAGLAVAVANIDA
                     VIALIRAAPDPAAARVALVERPWPAAEVAPLIELIAEPDREIDADGNYPMSEAQARAI
                     LDLRLQRLTGLERDKIAADLKEVTAKIVDYLDILRSHTRLLDIVRDELRQVAEQFGDD
                     RGRLTDIEEAEFEVDIEDLIEREDMVVTVTQNGYVKRVPLSSYRAQRRGGRGRAGMAT
                     READFVAQVFVASTHTPVLFFSSRGMAYVLKVYRLPLGAPQARGKPLINLLPLKEGER
                     VATLMPLPEDESSWDGLHVMFATSLGQVRRNALSDFTNIKANGKIAMKLEDDDGASLG
                     ELIAVQPCRDDQDVLLAAASGKCIRFPVSGVRVFAGRSSTGVRGMRLGEADEVISMSI
                     LEHFDVDTETRDGYLRYAAALRRGDEPPPPHDEAYDELAAAEQFILTVTENGYGKRSS
                     AYEYRIAGRGGQGIINIETSARNGRVVASFPVEGEDQILLVTDAGQLIRCPVADIRIA
                     GRNTQGVIVFKTADGERVVSVTRLADAGVDNGADERAEHGPEPQPASGAEAEDD"
     gene            complement(3906..4469)
                     /locus_tag="VGB88_05120"
     CDS             complement(3906..4469)
                     /locus_tag="VGB88_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646857.1"
                     /translation="MIPLRQGFGGLGAHAQNGVALPVIAVALAAVGALALIAAALQPM
                     RAERAGRERLLRTFDGLEAAVVEFAMVHKRLPCPADLAAATGLEAGPPGACADLTPGA
                     VPWRTLGVAAMLARDPWGRPISYRVAAALTTDRALLAPAAGAAGEAAALTSHGPDRAA
                     DGEVARVLSQAALLDRAGLAGRQDAAP"
     gene            complement(4466..5554)
                     /locus_tag="VGB88_05125"
     CDS             complement(4466..5554)
                     /locus_tag="VGB88_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9646858.1"
                     /translation="MTVTAGAAAAISTTTFRSDLPARSRSGFASARRPKALGRRAAKA
                     GPRPASRRRPAAGPGTRLPAGFTLVEMAIVILIIGLIAGIGADLMRRIERGNRIQRTE
                     AALAGVEEALALFAMRSKRLPCPADGALPEDDRNSGREDFEGGGPGDCLRQAGGTVPH
                     VALGIGGAAARDGWNRRLTYRVDPVLTRDRSAEGRNCASGAPGEACPCPGGGIDLSCI
                     DPRLIGDGRGGVDDVLVRFHGTGLGLEVCAAAGCADGSALMTRAAGNAAAYVLIGHGR
                     GGRGAFTTGGTLDEGAAGPVPVHERPNFNATPLDDAGKGPDAGGAFVDRERNDRDEGE
                     VEAFFDDFVARPSVLSVARAARLEPQAR"
     gene            complement(5499..5957)
                     /gene="ssb"
                     /locus_tag="VGB88_05130"
     CDS             complement(5499..5957)
                     /gene="ssb"
                     /locus_tag="VGB88_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018302435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9646859.1"
                     /translation="MAGSVNKVILVGNLGRDPEIRTMQNGGRVANLSLATSETWRDRQ
                     SGERREKTEWHRVVVFDDKLVDVAEKYLRKGAKIYVEGQLQTRKWQDQSGQDRYSTEV
                     VLQRFRGGLTMLDGRRDEAAMAGEPAAAEYGGGDGDGGGRGGDFDDDIPF"
     gene            6141..9065
                     /gene="uvrA"
                     /locus_tag="VGB88_05135"
     CDS             6141..9065
                     /gene="uvrA"
                     /locus_tag="VGB88_05135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9646860.1"
                     /translation="MKHDRDMLPSAKTIAVRGAREHNLKNVDVTLPRNRLIVITGLSG
                     SGKSSLAFDTIYAEGQRRYVESLSAYARQFLELMEKPDVDSIDGLSPAIAIDQKTTSR
                     NPRSTVGTVTEIYDYLRLLFARIGIPYSPATGLPIESQTVTQMVDGVLAMGDGARLYL
                     LAPIVRGRKGEYRKEIAELVKRGFQRLKVDGTVCEIDQVPALDKKRKHDIEVVVDRVV
                     VRKGLGNRLADSIETALELSDGLLFVEDADSGARKTFSARFACPVSGFTIDEIEPRLF
                     SFNSPYGACPECGGLGTLTYFDPELVAPNGSLSLRQGAIQPWSNASTQYYQQTLQSLA
                     KHYKFSLNKPFSDIPEKIQEVILYGSDGKDVPMTYDDGLRSYTTVRPFEGVIPNLERR
                     YNDSENQWLREELDRYQSETACDACGGHRLKPEALAVRVGEAHIGQVVEKGIGDAAGW
                     FRDIEPKLSPKQREIAGRILKEINLRLGFLVDVGLEYLTLARSASSLSGGESQRIRLA
                     SQIGSGLTGVLYVLDEPSIGLHQRDNARLLQTLRNLRDLGNTVIVVEHDEEAIRSADY
                     VVDIGPGAGEHGGQVVAEGSPAEVIADSASLTGQYLAGRRKIPVPATRRTGKRIGRGA
                     RVQELAVLGARENNLCDIDVAFPLGTFTCVTGVSGSGKSSLVVDTLYPALARRIMRAR
                     LHPGDHDSIEGIEHLDKVVDIDQSPIGRTPRSNPATYTGAFTPIRDWFAGLPEAKARG
                     YKPGRFSFNVKGGRCEACQGDGVLKIEMHFLPDVYVTCDNCHGKRYNRETLEVRYKDR
                     SIADVLDMTVEEATGVFQAVPAIRDKLETLRRVGLGYIRVGQPATTLSGGEAQRVKLA
                     KELSKRATGKTLYILDEPTTGLHFEDVRKLLEVLQALVETGNTVIVIEHNLEVIKTAD
                     WIIDLGPEGGDGGGRVVAKGTPEQVAACAGSYTGQYLARHLGARPRAARPAPVRKRRR
                     AS"
     gene            9062..10318
                     /locus_tag="VGB88_05140"
     CDS             9062..10318
                     /locus_tag="VGB88_05140"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005241103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9646861.1"
                     /translation="MTAANAHGPLAGTRIIDLSAMLSGPWATMILGDQGADVIKVEPP
                     DRGDHTRALGNRRGGLPANFLNINRSKRSVTIDLKRPEGRDLLLRLTRTADVVVQNFR
                     PGVVERLRIAEPDLRAVNPTLIYMSISGFGETGPYAGKPAYDPVIQALSGLTTVQAGS
                     DDARPRLVRTVLPDKLTGITAAQAITAALLARARTGEGQHVRLSMLDAVLSFLWASDM
                     GAQTYLHDPPDNQRAASFIDLIYQTADGYMTVAVMTDKEWAGLARAFGRPELVEDPLF
                     ATPAQRDRHVDERLALTQETLRARTTAEWLDVLEREGVPCAPALTRAEVVRHPQVEAS
                     GILFETDHPAAGRLRQTRSAARFEATPASHARGAPLLGEHTDEVLAELGLTAGEIAGL
                     RAAGVIGADGAGPADDEPQPEAAQRR"
     gene            10330..10659
                     /locus_tag="VGB88_05145"
     CDS             10330..10659
                     /locus_tag="VGB88_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9646862.1"
                     /translation="MAMIADAARRHVTLEKVRQMVLDALAGVDAQAYLFGSQARGDVW
                     TLSDIDVAIDHAEPLPVGLLARLREALEESTIPVRVDVVDLAEVDEEFRANVLAEGKR
                     WTASAKG"
     gene            10635..11060
                     /locus_tag="VGB88_05150"
     CDS             10635..11060
                     /locus_tag="VGB88_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HEX9646863.1"
                     /translation="MDRLGQRLSIAAQALATLRELSDLEAPSRVERDAAIQRFEYSFE
                     AVWKAGRLFLRTYEGVEAGSPKASVRASRDAGLLSRDQAEAALAMADDRNLTLHTYNE
                     TLAKEIFRRLAGHAALMGAWMDAMTGRLGTAKASGKGAG"
     gene            11092..12450
                     /gene="trmFO"
                     /locus_tag="VGB88_05155"
     CDS             11092..12450
                     /gene="trmFO"
                     /locus_tag="VGB88_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX9646864.1"
                     /translation="MSLRPVHVIGGGLAGSEAAWQLARAGVAVVLHEMRPVRSTPAHK
                     TAALAELVCSNSFRSDDAENNAVGLLHEEMRRAGSLVLRAADRHQVPAGGALAVDRDG
                     FSAAVEASLQSEPLVEIAREEVAGLPPAEWDSVIVATGPLTSPALADAIRDLTGADSL
                     AFFDAIAPIVHRDSVDMGIAWLQSRYDKPGPAGEPAAYVNCPLDAGQYRDFVAALLAA
                     DKTEFHEWERDTPYFEGCLPIEVMAERGPETLRFGPMKPVGLTNPHRPDEKPYAVVQL
                     RQDNALGTLFNMVGFQTKLKHGEQVRIFRTIPGLAGAEFARLGGIHRNTFLNSPKLLD
                     ASLRLRADARLRFAGQVTGVEGYVESAAIGLLAGRFAAGERLGRPISPPPPETAFGAL
                     LAHITGGGDAATFQPMNVNFGLFPPLAAPVGRKQRKPALARRALAALDSWLAAQARDA
                     AA"
     gene            complement(12505..>13103)
                     /locus_tag="VGB88_05160"
     CDS             complement(12505..>13103)
                     /locus_tag="VGB88_05160"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEX9646865.1"
                     /translation="NGMTRVSCVCGDDITGRGIAVMSCKRIVSAATVLAGACAAVFGT
                     AGASHAGDGPGVEVGILTCRSVEGTRLNLIFHSSVDLDCVFETPQGSERYVGESGVGL
                     GLDINWERTEEIRFAVISTTTESGIGDHSLAGKYVGGKASITAGRGVGAQGLVGVGPK
                     SFSLQPLAIETSEGFGLAAGLAYLFLEAPATEGASPTN"
BASE COUNT         1922 a         4722 c         4597 g         1862 t
ORIGIN      
        1 ccacctggcc ccagatggtg tacttgccgt ccaggaacgg cgccggctcg aagcagatga
       61 agaactggct gtcggcgctg tcggcgtgct gcgagcgggc catcgacacg gtgccgcgca
      121 ggtgcttggc gacgctcggc ggcgagaact cggccgggat gcgctggccc gagccgccgg
      181 tgccgtcgcc cttggggtcg ccgccctggg ccatgaagcc gtcgatcacg cggtggaagc
      241 tgagcccgtc gtagaagccc tggcgcacta gcgccttgat ccgggcgacg tgcttgggcg
      301 cgaggtccgg gcgcagctcg atcaccaccc ggccgctctc gagatccatg tacagcgtgt
      361 tctccgggtc ggcggcgctg ccgtcggctg aggccatgat caagctcgcg atcgcaaggc
      421 tggtgaacag acgtcggcgc attcggacct cccggtcatg agacggcccg ttcagccggt
      481 gccgccgatc tgcctcgata tatggtcatc caggcggcgc gcaacattgg gcggcacgaa
      541 ctgggtaacg tcgccgccga gcaggtagat ctccttgacc aggcgcgagg cgatgaactg
      601 gatgccctcg ctggccatca ggaacacggt ctcgatgtcg ggcgcaaggc gcgcgttcat
      661 gcccgccatc tggaactcgt actcgaagtc cgagaccgcg cgcaggcccc ggatgatgac
      721 cccggcgccc tgctgcgagg cgaagtggat caacaggttg tcgaacgccg tcacgctgac
      781 ccggctggcc acgtcgtggt ccatggcggc gaccgcctcg cgcagcatgg ccacccggtc
      841 gccgatctcg aacatcggcg ccttgccggc gttgaccgcg accccgatca ccaccttatc
      901 gacgaccctc agcgcccggc ggatgatgtc gagatggccg accgtggtcg gatcgaaggt
      961 gcccggatag atgccggtcc gcgggtcagt catcctcggc ctccgcgccg ctcgcgggct
     1021 gcggctcggg gccgtgctcg gcccgctcgt cggcgccgtt gtcgacaccg gcgtcggcga
     1081 gccgggtcac cgacaccacc cgctcgccgt cggcggtctt gaacacgatc acgccctggg
     1141 tgttgcgccc ggcgatgcgg atgtcggcga ccgggcagcg gatcagctgg ccggcgtcgg
     1201 tgaccagcag gatctggtcc tcgccctcga ccgggaacga ggcgaccacg cggccgttgc
     1261 gcgccgaggt ctcgatgttg atgatcccct gcccgccgcg cccggcgatg cggtactcgt
     1321 aggccgagct gcgcttgccg tagccgttct cggtgacggt gaggatgaac tgctcggccg
     1381 ccgccagctc gtcataggcc tcatcgtggg gcggcggcgg ctcgtcgccg cggcgcagcg
     1441 ccgcggcgta gcgcaggtag ccatcgcgcg tctcggtgtc gacgtcgaaa tgctcgagga
     1501 tcgacatcga gatcacctcg tcggcctcgc ccaagcgcat gccgcgcacc ccggtcgagc
     1561 tccgcccggc gaacacgcgc acgccggaga cggggaagcg gatgcacttg ccgctggccg
     1621 ccgccagcaa cacgtcctgg tcgtcgcggc agggctggac cgcgatcagc tcgccgaggc
     1681 tcgcgccgtc gtcgtcctcg agcttcatcg cgatcttgcc gttggccttg atgttggtga
     1741 agtcggacag cgcgttgcgc cgcacctggc cgagcgaggt ggcgaacatg acgtgcagcc
     1801 cgtcccagct cgactcgtcc tcgggcagcg gcatcaaggt ggcgacgcgc tcgccctcct
     1861 tcagcggcag caggttgatc aggggcttgc cgcgcgcctg cggggcgccg agcggcagcc
     1921 ggtagacctt gagcacatag gccatgccgc ggctggagaa gaacagcacc ggggtgtggg
     1981 tgctggcgac gaacacctgg gcgacgaagt cggcctcgcg ggtggccatg ccggcgcgcc
     2041 cgcgcccgcc gcggcgctgg gcgcggtagc tcgacagcgg cacccgcttg acatagccgt
     2101 tctgggtgac cgtcacgacc atgtcctcgc gctcgatcag gtcctcgatg tcgacctcga
     2161 actcggcctc ctcgatgtcg gtcagccggc cgcggtcgtc gccgaactgc tccgcgacct
     2221 ggcggagctc gtcgcggacg atatccaaca ggcgcgtgtg cgaacgcagg atgtccagat
     2281 agtcgacgat cttggcggtc acctccttga ggtcggcggc gatcttgtcg cgctcgagcc
     2341 cggtcaggcg ttgcaggcgc aggtccagga tcgcgcgcgc ctgggcctcg gacatcgggt
     2401 agttgccgtc ggcgtcgatc tcgcggtcgg gctcggcgat cagctcgatc agcggcgcca
     2461 cctcggcagc cggccacggc cgctcgacca gcgcgacgcg cgccgcggcg gggtcggggg
     2521 cggcgcggat cagcgcgatc acggcgtcga tgttggcgac cgcgaccgcc agcccggcga
     2581 ggatgtgcgc gcgctcgcgt gcgcgcgcca gctcgaactt ggtgcgccgc gtgaccaccc
     2641 gctcgcggaa gcgcaggaac gcctgcagca tgcgcttgag gtcgagctgc tcggggcggc
     2701 cgtcgacgag cgccagcgca ttgaccccga agctggtctg caacggcgtg taccggtaga
     2761 tctggctcaa caccacgtcg tagctcgcat cgcgcttgag ctcgaccacc acccgcacgc
     2821 cgtcgcggtc ggactcgtcg cgcaggtccg agacgccctc gatgcgccgc tcgcgcaccg
     2881 cctccgcgat ctgctcgacc aggcgcgact tgttgaccag gtacgggatc tcggtgatca
     2941 cgatcacccg gtcgtcgcgc tcggacatga cccggccgcg gaccacgacc gcgccgcgcc
     3001 cggtgtggta ggcggcgcgg atgccggcgc ggccgatgat gatgccgccg gtcgggaagt
     3061 cggggccggg gatgtgctcc atcagcgcct cgatcgacaa ggccgggtcg tcgatcagcg
     3121 ccaggcaggc ggcgaccacc tcgcgcaggt tgtgcggcgg gatgttggtc gccatgccga
     3181 ccgcgatgcc gccggcgccg ttgaccagca ggttcgggaa cctggccggc agcacccccg
     3241 gctcgttctc ggtgctgtcg tagttgggct ggaagtcgac agtgtccttg tcgaggtcct
     3301 cgagcagggc gccggcggcg cgctcgaggc gggcctcggt gtagcgcatc gccgcggcgc
     3361 ggtcgccgtc catcgagccg aagttgccct ggccgtcgat cagcggcagg cgcatcgaga
     3421 actcctgcgc catgcgcacc atggcgtcgt agatcgcctc gccgccgtgc gggtggtact
     3481 tgcccatcac gtcgccgacc acgcgcgccg acttgcggta cggatggctc cagtcgtagt
     3541 tgttctcgtg catcgagaac aggatgcggc ggtggaccgg cttcagcccg tcgcgcacgt
     3601 cgggcagcgc gcgggccacg atcacgctca tcgcgtaatc gaggtaggag cgcttcatct
     3661 cctcctcgat cgacaccggc gagatgtcga agggctgcgc ttgggccgag tcggtcacgg
     3721 cgtcacgaac ctgtcccggg gcgggcgggc ggggcgtcgc aggggtgaat cattggaccg
     3781 gaatactacc agatatgggg tgacagcggc caagagtccc gccagatatg gccgcgggcc
     3841 gaggcgcggt gaccgccccc cttcataccc cgtcgcgggc gcctttgcca gcccggatca
     3901 gggtgttacg gcgccgcgtc ctggcgcccg gcgaggccgg cgcggtcgag cagcgcggcc
     3961 tgcgacagca cgcgcgcgac ctcgccgtcg gccgcgcggt cggggccgtg gctggtcagc
     4021 gccgccgcct cgccggccgc cccggcggcg ggcgcgagca gcgcgcggtc cgtggtcagc
     4081 gcagcggcga cgcgatagga aatcggccgt ccccaggggt cgcgcgccag catcgccgcg
     4141 acgccgagcg tgcgccacgg caccgcgccc ggcgtgagat cggcgcaagc gccgggcggt
     4201 cccgcctcga gtccggtcgc ggccgcgagg tcggccgggc acggcaggcg cttgtggacc
     4261 atggcgaact cgaccaccgc cgcctcgagc ccgtcgaacg tgcgcagaag ccgctcgcgg
     4321 ccggcgcgct cggcgcgcat cggctggagc gcggcggcga tcagcgccag cgccccgacc
     4381 gccgccagag cgaccgcgat caccggcagc gcaacgccgt tctgcgcgtg cgcgccgagt
     4441 cccccgaagc cttggcgcag ggggatcacc gcgcctgcgg ctcgagccgc gccgcgcgcg
     4501 cgacgctgag cacgctcggc cgggcgacga agtcgtcgaa gaacgcctcg acctcgccct
     4561 cgtcgcggtc gttgcgctcg cggtcgacga aggcgccgcc cgcgtccggt cccttgccgg
     4621 cgtcgtcgag cggggtggcg ttgaagttgg ggcgctcgtg caccggcacc ggccccgccg
     4681 cgccttcatc cagggtgccg ccggtggtga acgcgccgcg gccgccgcgg ccgtggccga
     4741 tcaggacgta ggcggcggca ttgccggcgg cgcgggtcat cagcgcggag ccgtccgcgc
     4801 agccggcggc ggcgcacacc tcgagaccga ggccggtgcc gtggaaacgc accagcacgt
     4861 catcgacgcc gccgcggccg tcgccgatca ggcgcgggtc gatgcagctc aggtcgatgc
     4921 cgccgcccgg gcacgggcag gcctcgccgg gggcgccgct cgcgcagttg cgcccctcgg
     4981 ccgagcggtc gcgggtcagc accgggtcga cgcgataggt gaggcgccgg ttccagccgt
     5041 cgcgggcggc ggctccgccg atgccgaggg cgacgtgcgg cacggtgccg ccggcctggc
     5101 gcaggcagtc gccgggcccg ccgccctcga agtcctcgcg cccggagttc cggtcgtcct
     5161 cgggcagcgc gccgtcggcc gggcacggca ggcgcttgct gcgcatggcg aacagcgcca
     5221 gcgcctcctc gacgccggcg agcgcggcct cggtgcgctg gatccggttg ccgcgctcga
     5281 tccgccgcat caggtcggcg ccgatgccgg cgatcaggcc gatgatcagg atcacgatcg
     5341 ccatctcgac cagggtgaag ccggccggga gtcgagtccc gggcccggcg gcgggccgcc
     5401 ggcgcgaagc cggccggggg cctgccttcg ccgccctgcg gcccaaagcc ttcgggcgac
     5461 gggccgaagc gaagccgctt cggcttcgcg caggcaggtc agaacggaat gtcgtcgtcg
     5521 aaatcgccgc cgcggccccc gccgtcaccg tcaccgccgc cgtactcggc cgcggccggc
     5581 tcgccggcca tcgccgcctc gtcgcgacgg ccgtcgagca tggtcagccc gccgcggaag
     5641 cgctgcagca ccacctcggt ggagtagcgg tcctggcccg actggtcctg ccacttgcgg
     5701 gtctgcagct ggccttcgac atagatcttg gcgcccttgc gcaggtactt ctcggcgacg
     5761 tcgaccagct tgtcgtcgaa gaccaccacc cggtgccact cggtcttctc gcgccgctcg
     5821 ccgctctggc ggtcgcgcca ggtctcgctg gtggcgagcg acaggttggc gacccggccg
     5881 ccgttctgca tggtcctgat ttccgggtcg cggccgagat tgccgaccag gatcaccttg
     5941 ttgacgctgc cggccatggc ttaatgcccc tgtcgttcca ggtcgtggac ctgggctgcg
     6001 gcggcgagta tagacggccc gccgcccgga ggctatgggc cgcagggcgg tgcccgcaac
     6061 cgcccgccag ccgtttccgg gccgggccaa gccgaggtcc gttatgtccc ggccgccgct
     6121 tgcgcttata ctcgacgccg atgaagcacg atcgcgacat gctgcccagc gccaagacca
     6181 tcgccgtccg cggcgcgcgc gagcacaacc tcaagaacgt cgacgtcacc ctgccgcgca
     6241 atcggctgat cgtgatcacc gggctgtcgg gctcgggcaa gtcctcgctc gccttcgaca
     6301 ccatctacgc cgagggccag cgccgctacg tcgagagcct gtcggcctat gcccgccagt
     6361 tcctcgagct gatggagaag cccgacgtcg attcgatcga cggcctgtcg ccggcgatcg
     6421 ccatcgacca gaagaccacc tctcgcaacc cgcgctcgac tgtcggcacc gtgaccgaga
     6481 tctacgacta cctgcggctt ctgttcgcgc gcatcggcat cccctactcg ccggccaccg
     6541 gcctgccgat cgagagccag accgtgaccc agatggtcga cggcgtgctc gccatgggcg
     6601 acggcgcgcg gctctatctg ctggcgccga tcgtgcgcgg gcgcaagggc gagtaccgca
     6661 aggagatagc cgagctggtc aagcgcggct tccagcggct caaggtcgac ggcacggtct
     6721 gtgagatcga ccaggtgccg gcgctcgaca agaagcgcaa gcacgacatc gaggtggtgg
     6781 tcgaccgggt ggtggtgcgc aagggtctcg gcaaccgcct ggccgactcg atcgagaccg
     6841 cgctcgagct gagcgacggg ctgctgttcg tcgaggacgc ggactcgggc gcgcgcaaga
     6901 ccttctcggc gcgcttcgcc tgcccggtgt cgggcttcac catcgacgag atcgagccgc
     6961 ggctgttctc gttcaacagc ccctacggcg cctgcccgga gtgcggcggc ctcggcacgc
     7021 tgacctactt cgaccccgag ctggtcgcgc cgaacggctc cctgagcttg cgccagggtg
     7081 cgatccagcc ctggtcgaac gccagcacgc agtactacca gcagaccctc cagagcctgg
     7141 cgaagcacta taagttcagc ctaaacaagc cattttcaga tattcctgag aagatacaag
     7201 aggtcatcct gtacggctcg gacggcaagg acgtgccgat gacctatgac gacgggctgc
     7261 gcagctacac caccgtgcgc ccgttcgagg gcgtgatccc gaacctggag cgccgctaca
     7321 acgactccga gaaccagtgg ctgcgcgagg agctcgaccg ctatcagagc gagaccgcct
     7381 gcgacgcctg cggcggccac cgcctcaagc ccgaggcgct ggcggtgcgc gtcggcgagg
     7441 cgcatatcgg gcaagtggtc gagaagggaa tcggcgacgc cgccgggtgg ttccgcgaca
     7501 tcgagccgaa gctctcgccc aagcagcgcg agatcgccgg gcgcatcctc aaggagatca
     7561 acctcaggct cggcttcctg gtcgacgtcg gcctcgaata cctgaccttg gcacgcagcg
     7621 cgagcagcct gtcgggcggc gagagccagc gcatccgcct cgcctcgcag atcggctccg
     7681 ggctcaccgg cgtgctctac gtgctcgacg agccgtcgat cgggctgcac cagcgcgaca
     7741 acgcgcgcct gttgcagacg ctcaggaacc tgcgcgacct cggcaacacg gtgatcgtgg
     7801 tcgagcacga cgaggaggcg atccgcagcg ccgactacgt ggtcgacata ggcccgggcg
     7861 ccggcgagca cggcggccag gtggtcgcgg agggctcgcc ggcggaggtg atcgccgaca
     7921 gcgccagcct taccggccaa tacctcgccg ggcggcgcaa gatcccggtg ccggcgacgc
     7981 gccggacggg caagcgcatc ggtcgcggcg cgcgcgtcca ggagctcgcc gtgctcggcg
     8041 cccgcgagaa caacctgtgc gacatcgacg tcgcgttccc gctcggcacc ttcacctgcg
     8101 tcaccggcgt ctcgggcagc ggcaagtcga gcttggtggt cgacacgctc tacccggcgc
     8161 tggcgcgccg gatcatgcgc gcgcgactgc acccgggcga ccatgactcg atcgagggca
     8221 tcgagcacct ggacaaggtg gtcgacatcg accagtcgcc gatcgggcgc acgccgcgct
     8281 cgaaccccgc cacctatacc ggcgcgttca cgccgatccg cgactggttc gccggcctgc
     8341 ccgaggccaa ggcgcgcggc tacaagccgg ggcggttctc gttcaacgtc aagggcgggc
     8401 gctgcgaggc gtgccagggc gacggcgtgc tcaagatcga gatgcacttc ctgcccgacg
     8461 tctatgtcac ctgcgacaat tgccacggca agcgttacaa ccgcgagacc ttggaggtgc
     8521 gctacaagga ccgctcgatc gccgacgtgc tcgacatgac ggtcgaggaa gcgaccgggg
     8581 tcttccaggc ggtgccggcg atccgcgaca agctcgagac cttgcgccgg gtcggcctcg
     8641 gctacatccg cgtcggccag ccggcgacca ccttgtcggg cggcgaggcg cagcgcgtca
     8701 agctcgccaa ggagctgtcc aagcgtgcca ccggcaagac cctctacatc ctcgacgagc
     8761 cgaccaccgg actccacttc gaggatgtgc gcaagctgct ggaggtgctc caggctctgg
     8821 tcgagaccgg caacacggtg atcgtgatcg agcacaatct ggaggtgatt aagaccgccg
     8881 actggatcat cgacctcggg cccgagggcg gcgacggcgg cggccgcgtg gtcgccaagg
     8941 gcacgcccga gcaggtcgcc gcctgcgccg gcagctatac cggccagtac cttgcccgcc
     9001 atctgggcgc caggccgcgc gccgctcggc ctgcgcccgt gcgcaagcgg aggcgcgcgt
     9061 cgtgaccgcg gcgaatgcgc acgggccgct cgccggcacc cggatcatcg acctgagcgc
     9121 gatgctgtcg gggccgtggg cgaccatgat cctcggcgac cagggcgccg acgtgatcaa
     9181 ggtcgagccg ccggaccggg gcgaccacac gcgcgcgctc ggcaaccgcc gcggcggcct
     9241 gccggcgaac ttcctcaaca tcaaccgcag caagcgctcg gtgaccatcg acctcaagcg
     9301 gcctgaaggc cgcgacctgc tgctcaggct cacgcgcacc gccgacgtgg tggtccagaa
     9361 cttccgcccc ggcgtggtcg agcgcctgcg cattgccgag cccgacctgc gcgcggtcaa
     9421 cccgaccctc atctacatgt cgatcagcgg cttcggcgag accggcccct acgccggcaa
     9481 gccggcctac gatccggtga tccaggcgct gtccgggctg accacggtgc aggcgggctc
     9541 ggacgacgcg cgccccaggc tggtgcgcac ggtgctgccg gacaagctga ccgggatcac
     9601 ggcggcccag gcgatcaccg cggcgctgct cgcgcgcgcg cgcaccggcg agggccagca
     9661 tgtgcgctta tccatgctcg atgcagtgct gtcgttcctg tgggcgtccg acatgggcgc
     9721 gcagacctac ctccatgacc cgcccgacaa ccagcgcgcg gcgagcttca tcgacctgat
     9781 ctaccagacc gccgacggct acatgaccgt ggcggtgatg accgacaagg aatgggcggg
     9841 cctggcacgc gcgttcggcc gccccgagct ggtcgaggat ccgctcttcg ccactcccgc
     9901 ccagcgcgac cgccacgtcg acgagcgcct ggcgctgacc caggagacgt tgcgcgcgcg
     9961 caccaccgcc gagtggctcg acgtgctcga gcgcgagggc gtcccctgcg cgccggcctt
    10021 gacccgcgcc gaggtcgtcc gccaccctca ggtcgaggcg agcggcatcc tgttcgagac
    10081 cgaccacccg gcggccgggc gcctgcgcca gacgcgcagc gccgcccgct tcgaggcgac
    10141 gcccgcgagc catgcgcgcg gcgcgccgct cttgggcgag cacaccgacg aggtgctcgc
    10201 cgagctcggc ctgaccgcgg gcgagattgc gggactccgc gccgccggcg tgatcggcgc
    10261 cgacggcgcc gggcccgcgg acgacgagcc gcaacccgag gcggcacagc ggaggtaacg
    10321 gaggcgagga tggcgatgat cgcggatgca gcgcgacggc atgtgaccct ggagaaggtg
    10381 cggcagatgg tgcttgacgc gctcgccggt gtggacgcgc aagcttacct gttcggctcg
    10441 caggcgcgcg gcgacgtgtg gaccttgagc gacatcgacg tcgccatcga ccacgcggag
    10501 cccttgcccg tgggcctgct cgcccgcctg cgcgaggcgc tcgaggaaag caccatcccg
    10561 gtgcgcgtcg acgtggtcga cctcgccgaa gtcgacgaag agttccgcgc caacgtgctc
    10621 gccgaaggca agcggtggac cgcctcggcc aaaggctgag catcgccgcg caggcgctgg
    10681 cgacgctgcg cgagctgtca gacctcgagg ccccgtcgcg ggtcgagcgc gacgccgcga
    10741 tccagcgctt cgagtacagc ttcgaggcgg tctggaaggc gggccgcctg ttcctgcgga
    10801 catatgaagg cgtcgaggcg ggctcgccga aggcgtcggt tcgggcgtcg cgcgatgccg
    10861 gcctcttgtc gagggaccag gccgaggcgg cgctcgccat ggccgacgac cggaacctga
    10921 ccctccacac ctacaacgag acgctggcga aggagatctt tcgccgcctc gccggtcatg
    10981 ccgctctgat gggcgcctgg atggacgcca tgaccgggcg gctgggaaca gccaaggcgt
    11041 ccggcaaggg cgccggttaa cttgagcgcg ccgagcgcct agattggggc catgagcttg
    11101 cgcccggttc acgttatcgg cggcggcctc gccggcagcg aggcggcatg gcagctcgcg
    11161 cgcgctggcg tcgccgtggt gctgcacgag atgcgcccgg tgcgcagcac gcccgcgcac
    11221 aagaccgcgg ccttggccga gctcgtgtgc tcgaactcgt tccgctcgga cgacgcggag
    11281 aacaacgcgg tcgggctgtt gcacgaggag atgcgccgcg cgggctcgct ggtccttcgc
    11341 gccgccgacc gccatcaggt gccagcgggc ggcgcgcttg cggtcgaccg cgacggcttc
    11401 tcggccgcgg tcgaggcgtc gttgcaatcc gagccgctgg tcgagatcgc gcgcgaagag
    11461 gtcgcgggac tgccgcccgc cgagtgggac tcggtgatcg tcgccaccgg gccgctcacc
    11521 tcgcccgcgc tcgctgatgc gatccgcgat ctcacgggcg cggacagcct cgccttcttc
    11581 gatgcgatcg caccgatcgt ccaccgcgac tcggtcgaca tggggatcgc gtggctgcag
    11641 tcgcgctacg acaagcccgg ccccgccggc gagcccgccg cctatgtcaa ctgtccgctc
    11701 gatgccggcc agtaccgcga cttcgtcgcc gcgctgctcg ccgccgacaa gaccgagttc
    11761 cacgagtggg agcgcgacac gccgtatttc gagggctgcc tgccgatcga ggtgatggcg
    11821 gagcgcggcc ccgagacctt gcgcttcggg ccgatgaagc cggtcgggct caccaacccg
    11881 caccggccgg acgagaagcc ctacgcggtc gtccagctgc gccaggacaa cgcgctcggc
    11941 accctgttca acatggtcgg cttccagacc aagctgaagc acggcgagca ggtgcgtatc
    12001 ttccgcacca ttccgggcct cgcgggcgcc gagttcgcgc gcctcggcgg catccaccgc
    12061 aacaccttcc tcaacagccc gaagctgctc gatgccagcc tgcggctgcg cgccgatgca
    12121 aggctgcgct tcgccggcca ggtgaccggg gtcgagggct atgtcgagag cgccgcgatc
    12181 ggcctgctcg ccggccgctt tgcggcgggc gagcggctcg gccgcccgat ctcgccgccg
    12241 ccgcccgaga ccgcgttcgg cgcgctgctc gcccacatca ccggcggcgg cgacgccgcc
    12301 accttccagc cgatgaacgt caacttcggc ctgttcccgc cgctcgccgc accggtcggg
    12361 cgcaagcagc gcaagcctgc cctcgcccgc cgcgcactcg ccgccctcga ctcctggctc
    12421 gccgcccagg cccgcgacgc cgccgcgtga gcccgcgcac gacgtccttc aggtcagccc
    12481 gcggaacggg ccttcggcag acaatcagtt ggtcggcgaa gccccctcgg tggccggcgc
    12541 ctcgagaaac agatacgcca ggccggcggc caggccaaaa ccctcgctgg tctcgatcgc
    12601 gagcggctgc aggctgaaac tcttcggccc gaccccgacc aggccctgtg cgccgacacc
    12661 ccggccggca gtgatcgagg ccttgccgcc gacgtacttg cccgcaagcg agtgatcgcc
    12721 gatgccggac tcggtggtgg tcgagatcac cgcgaaccgg atctcttccg tgcgctccca
    12781 atttatgtcg agaccgaggc cgactccgct ctcgccgaca tagcgctcgc tgccctgcgg
    12841 cgtctcgaac acgcaatcga gatcgacgga ggaatggaat atcaggttga gccgggtgcc
    12901 ttcgacgctg cggcacgtca ggatgccgac ctcgacgccg gggccgtctc cggcatggga
    12961 cgcaccggcc gtgccgaata cggcggcaca cgcgcccgcc aacacggtgg cggcactaac
    13021 aattcgcttg caagacatga ctgcaattcc tcgcccggtg atgtcgtccc cgcaaacgca
    13081 acttacccgc gtcatgccgt tca
//
LOCUS       DASWHU010000140        19418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1922092, whole genome shotgun sequence.
ACCESSION   DASWHU010000140 DASWHU010000000
VERSION     DASWHU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922092"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGB88_05165"
     CDS             <1..194
                     /locus_tag="VGB88_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646866.1"
                     /translation="LGGGRPGGEQADDGGQRNACQAGGTVAATGCRGEYSAALEAAPG
                     DGRALRDGPAGLLSVRPLF"
     gene            complement(287..1207)
                     /locus_tag="VGB88_05170"
     CDS             complement(287..1207)
                     /locus_tag="VGB88_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX9646867.1"
                     /translation="MLRAAMRSGLLHIAVIVAAYVGLPHLLEEAPTAENVIVVDMVQV
                     AEQRNLPNRMIEPEPQPEPEPEPVKQEAPKVEPPPPPPPPEAAEAPPPVPAPEPAAEP
                     TPPPEPEIAAPAPAPEPTPEPEPEPLAAEAAPLPAEIDRPIRKPEPPSPLDFEQALKS
                     LEAAASEPPQPRREPEPEPAPPAAADPIETLLAAADTPFRRDAPLSMTEIDNIREQIR
                     RNWNVPAGARDAQITEVTLRIQLAQDGTVRRVEVVDQERMRRDPFFRTMAESAVRAVN
                     KTGRIVNLSPDKYHLWRDMVVTFDPKEMFG"
     gene            complement(1212..1670)
                     /gene="tolR"
                     /locus_tag="VGB88_05175"
     CDS             complement(1212..1670)
                     /gene="tolR"
                     /locus_tag="VGB88_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX9646868.1"
                     /translation="MALPAAGARTIGPGRPRRYRPMSEINVTPFVDVMLVLLVVFMVT
                     APLLTVGVEVDLPESSANHLPGDDEPLTVTIDRTGALFLQELEIGLDELVPKLRAIAA
                     ERADMRIFVRGDKAIDYGRVMEVMGTVNAAGFTRVGLVTELPRLAEDGGR"
     gene            complement(1670..2392)
                     /gene="tolQ"
                     /locus_tag="VGB88_05180"
     CDS             complement(1670..2392)
                     /gene="tolQ"
                     /locus_tag="VGB88_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX9646869.1"
                     /translation="MEAVELQAAGVVTDLSIWGLVRQADFVVKLVLMILLLASFWCWA
                     IIFDKSRQLKRLRAKAEEFEESFWSGGSLEELYERIGGAPDHPLAMLFSVAMREWSLA
                     TARGMPIADMRSPSGLQQRIDRVMTVAFQREMEGLEQRLGFLATVGSTAPFIGLFGTV
                     WGIMNSFQSIAVTKNTSLAVVAPGIAEALLATALGLVAAIPAVVAYNKLSNDLNRYAG
                     RLETFAGEFGALLSRQLAERGA"
     gene            complement(2380..2859)
                     /gene="ybgC"
                     /locus_tag="VGB88_05185"
     CDS             complement(2380..2859)
                     /gene="ybgC"
                     /locus_tag="VGB88_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX9646870.1"
                     /translation="MTVDPGGGRVHVWPVRVYYEDTDAAGIVYYANYLKFAERARTEL
                     LRSCGIDHSRLAAEQGLAFAVRDCRIEYQAPARLDDALEVRTRVLALSGAAISIEQSV
                     HRGREALARIEMRIVCVRGDGRPGRLPGAVKAAVAPFRCGRDRAVQGNETMAGTWKR"
     gene            complement(2849..3913)
                     /gene="ruvB"
                     /locus_tag="VGB88_05190"
     CDS             complement(2849..3913)
                     /gene="ruvB"
                     /locus_tag="VGB88_05190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9646871.1"
                     /translation="MSATGSEDARLVASRFESADAAEAGLRPETLDDFIGQDELRANL
                     RVFVDAARARREALDHVLLFGPPGLGKTTLAQIVARELGVGFRASSGPVIARPGDLAA
                     LLTNLEPRDVLFLDEIHRLSPQVEEILYPAMEDFQLDLIIGEGPGARSVRIDLNPFTL
                     IGATTRSGLVGTPLRDRFGIPLRVRFYRPEELEAVVRRAARVMAMTLTEDGAHEIACR
                     ARGTPRVAVRLLRRVRDFATIAGAEEVDFAAAADALARLEVDARGLDAMDRRYLACIA
                     NHFGGGPVGVDTMAAALSEERDTIEDVIEPYLMQQGFVQRSPRGRLLTELGFRHLGLE
                     PRRDALSQLNLLGTEPADDG"
     gene            complement(3910..4512)
                     /gene="ruvA"
                     /locus_tag="VGB88_05195"
     CDS             complement(3910..4512)
                     /gene="ruvA"
                     /locus_tag="VGB88_05195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9646872.1"
                     /translation="MIARLRGRLDSNDEAGVVVEVGGVGYLVFCPRRTCESLPGPGAE
                     VDLLIETHVRDDHIHLYGFASAAERAWFRRLLTVQGVGARVAMAVLSVLSPEQLATAV
                     AAQDAAALRRVGGVGPKVAGRIVAELKDKVVAMAVAPAASAGPVPGSGASGEAVSALV
                     NLGYGRSEAFTAIAAAARRLGADGDVASLIRHGLRELASA"
     gene            complement(4509..5036)
                     /gene="ruvC"
                     /locus_tag="VGB88_05200"
     CDS             complement(4509..5036)
                     /gene="ruvC"
                     /locus_tag="VGB88_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9646873.1"
                     /translation="MRLLGLDPGLRATGWGVLDCAGTRLAFVACGTVRTDARAPLAAR
                     LATLYRGLAQVILTYAPASAAVEETFVNRNPASALKLGQARGVALLAPASAGLAVAEY
                     HNRQVKKALVGAGRADKRQVGLMVRHLLPGCRPDSDDAADALAVAICHAHHAATTNRW
                     RDDAAAVWLAAGSGS"
     gene            complement(5086..5841)
                     /locus_tag="VGB88_05205"
     CDS             complement(5086..5841)
                     /locus_tag="VGB88_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9646874.1"
                     /translation="MAGHSQFKNIMYRKGAQDAKRAKMFAKLARELTVAAKTGLPDPA
                     TNPRLRAAIAAARVANMPKDNIERAIKKGAGGGDEASFEEIRYEGYGPGGVAVIVEAL
                     TDNRNRTAAAVRSAFTKHAGSLGESGSVNFMFHRAGTIRYPAGAASADAMFEAAVEAG
                     ADDVDSGSSGHVVTCRPERLNDVREALAQRFGAPSSAGLEWLPQNTVEVDEGEAATLL
                     RMIEALEDSDDVQSVSANFEIPDEVMQRLSAGA"
     gene            complement(6045..6860)
                     /locus_tag="VGB88_05210"
     CDS             complement(6045..6860)
                     /locus_tag="VGB88_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX9646875.1"
                     /translation="MRILFCGDIVGSSGRAAIAEHLPRLKRELEIDFVVANGENAAGG
                     FGITAPIADDLFEAGVDVITTGNHVWDQREVVTLLPREPRLLRPCNYPPATTPGQGAG
                     VFNTQRGKRVLVINVMGRLFMDPLDDPFACLDQAFANHRLGADIDAAVIDVHAETSSE
                     KQAIGHFLDGRASLVVGTHTHVPTGDAMVLAGGTGFQTDAGMCGDYDSVIGSDKGMWV
                     QKFRSKMPVGRITPATGAGTLCAVFVETDEATGLARRIGPLACGGRLKPRWPL"
     gene            complement(6857..7444)
                     /locus_tag="VGB88_05215"
     CDS             complement(6857..7444)
                     /locus_tag="VGB88_05215"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX9646876.1"
                     /translation="MPLAPAAEKARLRAECLERRARIGAGARAEAAARIADNVLAAVA
                     LAPAAVVSGYWPFADEVDPRPLMIALHARGHPLCLPVAERPGTPLAFRAWAPGEALTE
                     GVFATSVPAPDRPRVVPDCLLVPLLAFDRDGYRLGFGAGYYDLTLAALEGSGRLSVGL
                     AFAAQEVSRLPREPHDMALDWIVTERGALHIGAAR"
     gene            complement(7457..7614)
                     /gene="ssrS"
                     /locus_tag="VGB88_05220"
     ncRNA           complement(7457..7614)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGB88_05220"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(7622..7957)
                     /locus_tag="VGB88_05225"
     CDS             complement(7622..7957)
                     /locus_tag="VGB88_05225"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX9646877.1"
                     /translation="MPQVKVQINGRSYAVQCEDGEQEHLRQLGAYIDAVVRNLLKRGN
                     VNLGQVGDTHLMVLAALTVADELSDAYDKTGPGGGGVGGGKTDFATALDGMAERLDRI
                     AGRLETLYV"
     gene            complement(7982..8251)
                     /locus_tag="VGB88_05230"
     CDS             complement(7982..8251)
                     /locus_tag="VGB88_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HEX9646878.1"
                     /translation="MATIEQARGRIEAALDRLEAAVEHAASAGAGSGRRAASAELAGV
                     RADRDRLAAELKALQQRYDDLKALTDTATDRLDQTIARVRGMLGG"
     gene            8460..10472
                     /gene="tkt"
                     /locus_tag="VGB88_05235"
     CDS             8460..10472
                     /gene="tkt"
                     /locus_tag="VGB88_05235"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9646879.1"
                     /translation="MTVPKASPAASDVVAAPSQRDLANAIRALAMDSVEAAKSGHPGM
                     PMGMADVATVLVHRFLAFDAAAPDWPDRDRLVLSAGHGSMLLYAILYLTGTAGMTIDD
                     IRNFRQLGSPAAGHPEYGHAPGIETTTGPLGQGLANAVGMALAERLMAARYGADLVDH
                     FTYVIASDGDLMEGISHEAASLAGHLRLAKLIVLFDDNHVSIDGPTELAVSDDQPARF
                     RALGWDVAACDGHDAEAVAAAIARARRTDRPSLIACRTTIGYGAPTKQGTAATHGAPL
                     GADEVALARARLGWPSPPFEVPAAVLGAWRTAGRRGAAARVAWQARLAAAPARARFER
                     VLAGTLPDGLAPALAALKARLASDAPRIATRKASEIALEVINAEVECTVGGSADLTGS
                     NNTRTKGMAAVAPGDFAGRYVFYGVREHAMAAVMNGIALHKGLIPYGGTFLIFSDYAR
                     PAMRLAALMGLRVIYVLTHDSIGLGEDGPTHQPVEHLASLRAMPNLWVFRPADAIECA
                     ECWELALARSSGPSVIALTRQGVPCVRAGAGPANRSAEGAYEIAAAEGRAQATILATG
                     SEVAVALAARAVLEGEGIATRVVSMPCWELFAEQPAAKQAEVLGPGTVRVAVEAASPQ
                     GWARWGVDETTMIAMRTFGASAPAERLFAHFGITAEAVAEAVRRKL"
     gene            10508..11512
                     /gene="gap"
                     /locus_tag="VGB88_05240"
     CDS             10508..11512
                     /gene="gap"
                     /locus_tag="VGB88_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9646880.1"
                     /translation="MAIRIGINGFGRIGRLVMRAAAEAGRNDVQVVGINDLGPVETNA
                     HLFRHDSVHGRFAGEVKVTGDAIDIGAGAIKVTALREPAKLPWRELGVDVALECTGRF
                     TDRAAAAAHLEAGARKVLVSAPAKGADLTVVFGVNHDRLTPEHQVVSNASCTTNCLAP
                     VAKVLNDAVGIERGFMTTIHAYTGDQPVLDTLHKDLHRARAAAMSMIPTSTGAARAVG
                     LVLPELAGKLDGTAIRVPTPNVSLIDLTFAAARKTSVDEINDAARKAAAGPMRGVLAT
                     CDAPLVSMDFNHDPASSTFDLGQTQVIDGRFVRVLAWYDNEWGFSNRMNDVAAVLGAL
                     "
     gene            11688..12887
                     /locus_tag="VGB88_05245"
     CDS             11688..12887
                     /locus_tag="VGB88_05245"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9646881.1"
                     /translation="MEKLSLLRDLDVGGRRVLLRLDLNVPVDDHHRITDDTRIRRALP
                     TLRELCERGARTVILSHFGRPKGRRVEEMSLGFLSAPLSDALARDVAVASDCIGKGPE
                     AVAAALEPGAVALFENLRFHAGEEANDAGFADALARSGDAYVNDAFSVCHRAHASVVG
                     LPARLAHAAGQALEAELEALDAALGHPARPLLAIVGGAKVSTKIGVLANLIGKVDLLA
                     IGGAMANTFLAVQGHGVGASLCEPELADAARRIMADAQAAGCEIILPVDAVVADALRA
                     GAEPAIVAVDAVPAGKMILDIGPASADAIGNRIPDCRTLLWNGPVGAFEVHPFDTSTV
                     LLARTAATMTRAGALVSVAGGGDTVAALAHAGAEAGFTYVSAAGGAFLEWLEGKTLPG
                     VAALMAD"
     gene            12900..13301
                     /locus_tag="VGB88_05250"
     CDS             12900..13301
                     /locus_tag="VGB88_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646882.1"
                     /translation="MVIVHGPDHIEACFAAAEQSGRALSALVAAWAGAAYGPLYLAAA
                     VAAARARHPRAEVRIALDCADDAGRAMAALRSGWQRLAFSGRADVRARIAGMAEAQGA
                     MLIESHTGPVLDLAGEAAPEAALARRLADCK"
     gene            13363..14283
                     /locus_tag="VGB88_05255"
     CDS             13363..14283
                     /locus_tag="VGB88_05255"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX9646883.1"
                     /translation="MRVTQRVRRILANYESDNPGTKANLARILMHGRLAGSGRLVILP
                     VDQGFEHGPARSFAPNPPAYDPHYHFQLAIDAGLSAYAAPLGMIEAGADSFAGAIPTI
                     LKLNSANSLAVTKDQAVTGSVADALRLGCAAIGFTLYPGSEDQYEMIEELRELAEEAK
                     AAGLAVVVWSYPRGGKLSKEGETAIDVTAYAAQLAALLGAHIIKVKPPTEHIEQDEAR
                     KVYQAQGIDVSSLRARIAHVVQSAFNGRRLVVFSGGAAKGTEGLLDEIREIRDGGATG
                     SIIGRNSFQRSRKDALALLDTIVRIYQGKA"
     gene            14280..14954
                     /gene="thiE"
                     /locus_tag="VGB88_05260"
     CDS             14280..14954
                     /gene="thiE"
                     /locus_tag="VGB88_05260"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX9646884.1"
                     /translation="MSRERCRLYLITPPGLGHDRAALDAFAIQLAGALAAGDVACVQL
                     RLKGADDAAVLRAAAALMPPCHRREVAVLVNDRADLAARAGADGVHIGAEDGGYARAR
                     ALVGEGRIVGVSCYDSRHLALEAAEAGADYVAFGAFFPTATKQPPAQAGLDLLRWWQE
                     MITVPCVAIGGITADNCAPLVEAGADFLAVISGVWDYPDGPAAAVRAFNAEIDRAMAR
                     ADIDSA"
     gene            complement(14964..15704)
                     /locus_tag="VGB88_05265"
     CDS             complement(14964..15704)
                     /locus_tag="VGB88_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9646885.1"
                     /translation="MDPASNIGLLTAAGAGLVSFLSPCVLPLVPGYLSYIAGRSVVDP
                     DEPRDARFRLTVVVLAFAFVLGFSTVFVLLGASATAAGQLLLAYREQLTLVGGAIVAL
                     FGIVTLGLIKPVWLLRDLRLTLPVVGGRPESAYLLGLAFAFGWTPCIGPILGAILTVG
                     ATSATVSGGVTLLGFYSLGLGVPFLISALLVERIVYWLRSARRAGMVLQLAAGGVMVA
                     IGLAMLTGQLSVLSYWFLDAVPLLGEIG"
     gene            complement(15727..16530)
                     /locus_tag="VGB88_05270"
     CDS             complement(15727..16530)
                     /locus_tag="VGB88_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9646886.1"
                     /translation="MPKYRVATTLVPVSLLAVLLAAGAAFWLWADPGFERAGGERTDV
                     AGVPEDEFDRRVRDYLMRNPQVILESVTALRDREQAEQETRAITALDERAGEILRDPD
                     SPVGGNPAGDVTLVEFFDYNCPYCRRVAPVVQEAVAADGELRVVYKEFPILGANSVTA
                     AKAALAAHRQGKYVAFHTAMMVAKRADDDAVTDIANEIGLDLEALRRDMEDPAIAAAI
                     ERNMRLALDLRITGTPGFVVGQDIIRGATDLGTLQGSIAKARGSSDVEG"
     gene            complement(16552..17283)
                     /locus_tag="VGB88_05275"
     CDS             complement(16552..17283)
                     /locus_tag="VGB88_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9646887.1"
                     /translation="MRLLRRLPILLVLSLAAVAPAAAHSLDELEGELRDREQYFQPVD
                     KPTPDFTLLGSGGERVSPADLLGKVVVLHFIYTSCPDVCPLHADRLADIQAMVNQTPM
                     TDQVRFVTITTDPGNDTPDVLRDFGVAHGLDPVNWLYLTAGPEQPEDVTRNLAEQFGH
                     KFTKTEDGYQIHGVVTHVIDSRGRWRANFHGLKFEPTSLVVFLNALVNDITNPHGHDE
                     PAGVLDWLWDLFGQLESRWSRSAKS"
     gene            complement(17345..17653)
                     /locus_tag="VGB88_05280"
     CDS             complement(17345..17653)
                     /locus_tag="VGB88_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646888.1"
                     /translation="MHKSTRTLALAAVLAGGLAAAPALYAHDSDDSRGSMMGPGMMGQ
                     GGMMGQGGMMGMMNMMGQMGRMTDQCAQMMEGTTGDRGAGRPNEQWRRDGPTAPDTKR
                     "
     gene            complement(17646..18038)
                     /locus_tag="VGB88_05285"
     CDS             complement(17646..18038)
                     /locus_tag="VGB88_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646889.1"
                     /translation="MLNGLRRHLSGRRGIMIAAGAALAIGLAANWSWLVAIGLAPLLL
                     GVLPCLAMCALGLCMNKMAGRSCAAGTARPVEPGATATSRVTPAETAVVEWQVANGDG
                     LVGEPVVFAAPGVAIDAQPQETRRNPDA"
     gene            complement(18272..18679)
                     /locus_tag="VGB88_05290"
                     /pseudo
     CDS             complement(18272..18679)
                     /locus_tag="VGB88_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(18879..>19418)
                     /locus_tag="VGB88_05295"
     CDS             complement(18879..>19418)
                     /locus_tag="VGB88_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX9646890.1"
                     /translation="PLLAFFGLAVVFAWGLGRDPSQIPSVLVGKPVPEFSLPPVQGRE
                     LGLASGDLVGEVSLVNVFASWCTACRAEHPLFMRLADEGVVPIHGLNYKDRPADAAGW
                     LDSLGDPYTRTGADIDGRVGIDFGVYGVPETFVISPDGGIAHKHIGPVTQKDLDRTIL
                     PLVARLREDGRRANASDDP"
BASE COUNT         2792 a         6966 c         6956 g         2704 t
ORIGIN      
        1 agctgggcgg aggccggccg ggcggcgagc aggccgacga tggcggccag cgcaacgcgt
       61 gccaggcggg cggaacggtc gctgcgaccg ggtgtcgggg cgagtactcg gcggcgctcg
      121 aggcggcgcc gggcgacggc cgcgcgcttc gcgacggccc ggcgggcctc ctcagcgtga
      181 ggccgttgtt ttgaaatgaa aaacctcgtc ctgaggagcg agcgaagcga gcgtctcgaa
      241 ggacgtggcc tgtaacgcgc agttgcgaat ccgggtacta gatgcatcag ccaaacattt
      301 cctttggatc gaacgtcacc accatgtcac gccacaggtg gtacttgtcg ggcgacaggt
      361 tgacgatgcg cccggtcttg ttgaccgcgc gcacggcgct ttcggccatg gtgcggaaga
      421 acgggtcgcg gcgcatgcgc tcctggtcga ccacctcgac ccggcggacg gtgccgtcct
      481 gggcgagctg gatgcgcagc gtcacctcgg tgatttgggc gtcgcgcgcg ccggcgggca
      541 cgttccagtt gcgccggatc tgctcgcgga tgttgtcgat ctcggtcatg ctcagcggcg
      601 cgtcgcgccg gaacggcgtg tcggccgcgg ccagcagcgt ctcgatcgga tcggccgcgg
      661 ccggcggcgc gggctccggc tccggctcgc gccgcggctg cggcggctcc gaggcagccg
      721 cctcgaggct tttcagcgcc tgctcgaaat cgagcggcga cggcggctcg ggcttgcgga
      781 tcggccgatc gatctccgcc ggcagcggcg ccgcctcggc cgcgagcggc tcgggctcgg
      841 gctccggcgt cggctccggc gcgggcgcgg gcgccgcgat ctcgggctcc ggcggcggcg
      901 tcggctcggc ggccggttcc ggcgcgggca cgggcggcgg cgcctcggcg gcctcgggcg
      961 gcggcggcgg cggcggtggc tcgaccttcg gcgcttcctg cttgacaggc tcgggctcag
     1021 gctcgggctg gggctcgggc tcgatcatgc ggttgggcag gttgcgctgc tcggcgacct
     1081 ggaccatgtc gaccacgatc acgttctccg ccgtcggcgc ctcctcgagc agatggggca
     1141 ggccgacata ggcggcgacg atcaccgcga tgtgcagcag gcccgatctc atcgccgcgc
     1201 gcagcatggc ctcagcgccc gccgtcctcg gccagccgcg gcagctcggt gaccagcccg
     1261 accctggtga agccggcggc attcacggtg cccatcacct ccatcacccg gccgtagtcg
     1321 atcgccttgt cgccgcgcac gaagatgcgc atgtccgccc gctcggccgc gatcgcgcgc
     1381 agcttcggca ccagctcgtc gaggccgatc tcgagctcct gcaggaacag ggcgccggtg
     1441 cggtcgatgg tgacggtcag cggctcgtcg tcgccgggca ggtggttggc ggaggattcg
     1501 ggcaggtcga cctcgacgcc gaccgtcagc agcggcgcag tgaccatgaa caccaccagc
     1561 agcaccagca tcacgtcgac gaacggcgtg acgttgatct cggacatcgg ccggtagcgc
     1621 cgcggcctgc ccgggccgat ggtgcgcgcg ccggcggcgg gcagcgccat cacgcgcccc
     1681 gctcggccag ctggcgcgag agcagcgcgc cgaactcgcc ggcgaaggtc tcgagccggc
     1741 cggcatagcg gttgaggtcg ttgctcagct tgttgtaggc gaccaccgcc gggatcgccg
     1801 cgaccaggcc gagggcggtg gcgagcagcg cctcggcgat gccgggcgcg accaccgcca
     1861 gcgaggtgtt cttggtcacc gcgatcgact ggaagctgtt catgattccc cacacggtgc
     1921 cgaacaggcc gatgaacggc gcggtcgagc cgacggtggc aaggaagccc aggcgttgct
     1981 cgaggccctc catctcgcgc tggaaggcga ccgtcatcac ccggtcgatg cgctgctgga
     2041 ggccggacgg ggagcgcatg tcggcgatcg gcatgccgcg cgcggtggcc agcgaccact
     2101 cgcgcatggc gaccgagaac agcatcgcca gcgggtggtc cggggcgccg ccgatgcgct
     2161 cgtacagctc ctcgagcgag ccgcccgacc agaacgactc ctcgaactcc tcagccttgg
     2221 cgcgcagccg cttcagctgg cgcgacttgt cgaagatgat cgcccagcac cagaacgagg
     2281 ccagcagcag gatcatcagc accagcttga ccacgaagtc ggcctggcgc accaggcccc
     2341 agatcgacag gtcggtgacc acgcccgcag cctgcagctc taccgcttcc atgtacccgc
     2401 catcgtctcg ttaccctgca ccgcccggtc gcggccgcac cggaacggcg ccaccgccgc
     2461 cttgaccgcg cccggcagcc gccccgggcg gccgtcgccg cgcacgcaga cgatgcgcat
     2521 ctcgatgcgc gccagcgcct cgcggccgcg gtgcaccgac tgctcgatgc tgattgccgc
     2581 gcccgacagg gcaaggacgc gcgtgcgcac ctcgagcgca tcgtcgaggc gcgccggcgc
     2641 ctgatactcg atccggcagt cgcgtaccgc gaacgcaagc ccctgctcgg ccgccaggcg
     2701 cgagtggtcg atgccgcagc tgcgcagcag ctcggtgcgc gcccgttccg cgaacttcag
     2761 atagttcgcg taataaacga tgccggcggc atcggtgtcc tcgtaataga cccgcaccgg
     2821 ccatacgtgg actctgccgc cgcccggatc aaccgtcatc ggcgggctcc gtgcccagca
     2881 ggttgagctg gctgagcgcg tcgcgccgcg gctcgagccc gaggtggcgg aatccgagct
     2941 cggtcaagag ccgcccgcgc gggctgcgct ggacgaagcc ctgctgcatc aggtagggct
     3001 cgatgacgtc ctcgatcgtg tcgcgctctt ccgacagcgc cgccgccatg gtgtcgacgc
     3061 ccaccgggcc gccgccgaaa tggttggcga tgcaggcgag gtagcgccgg tccatcgcgt
     3121 cgaggccgcg ggcgtcgacc tcgagccggg caagcgcgtc ggcggcggcg gcgaagtcga
     3181 cctcctcggc gccggcgatg gtggcgaagt cgcgcacccg cctgagcagg cgcaccgcca
     3241 ccctcggcgt gccgcgcgcg cggcacgcga tctcgtgcgc gccgtcctcg gtcaaggtca
     3301 tcgccatcac ccgcgccgcg cggcggacca ccgcctcaag ctcctcgggc cggtagaagc
     3361 ggaccctgag cggaatcccg aagcggtcgc gcagcggcgt gccgaccagg ccggagcggg
     3421 tggtggcgcc gatcagggtg aacgggttga ggtcgatgcg caccgagcgc gcgcccgggc
     3481 cctcgccgat gatcaggtcg agctggaagt cctccatcgc cggatagagg atctcttcca
     3541 cctgcggcga caggcgatgg atctcgtcga ggaacagcac gtcgcgcggc tcgagattgg
     3601 tcagcagcgc ggcgaggtcg ccggggcgcg cgatcaccgg gccggagctg gcgcggaagc
     3661 cgacgccgag ctcgcgggcg acgatctggg cgagggtggt cttgccgagc cccggcgggc
     3721 cgaacaagag cacgtggtcg agcgcctcgc ggcgggcgcg cgcggcgtcg acgaacacgc
     3781 gcaggttggc gcgcagctcg tcctggccga tgaagtcgtc gagggtctcg gggcgaagcc
     3841 cggcctcggc cgcgtccgcg ctctcgaacc gggacgcgac caggcgcgca tcctccgacc
     3901 cggtcgcgct catgccgacg ccagctccct caggccgtgg cggatcaggc tggccacgtc
     3961 accgtccgcg cccagcctgc gggcggcggc ggcgatggcg gtgaacgcct cgctgcgccc
     4021 gtagccgaga ttgaccagcg ccgagaccgc ctcgcccgac gcgccgctgc cgggcaccgg
     4081 cccggcggac gccgcgggcg ccacggccat ggccacgacc ttgtccttga gctcggcgac
     4141 gatgcggcct gcgaccttgg gcccgacccc gcccacgcgc cgcaacgcgg ccgcgtcctg
     4201 ggcggcgacg gcggtggcaa gctgctccgg gctgagcacc gacagcaccg ccatcgcgac
     4261 ccgcgcaccg accccttgca cggtcagcag ccggcggaac cacgcccgct ccgcggcgct
     4321 ggcgaagccg tagagatgga tgtggtcgtc gcggacatgg gtctcgatca gcaggtcgac
     4381 ctcggcgccg ggcccaggca gcgactcgca ggtacgcctg ggacagaaca ccagatagcc
     4441 gaccccgccg acctcgacca cgacgcccgc ctcgtcgttc gaatcgagcc ggccgcgcag
     4501 gcgcgcgatc acgacccgct cccggccgca agccacaccg ccgccgcatc gtcgcgccag
     4561 cggttggtgg tggcggcgtg gtgggcatgg cagatcgcca ccgccagcgc gtcggccgca
     4621 tcgtcgctgt cggggcggca gccgggcagc aggtggcgga ccatcaggcc gacctggcgc
     4681 ttgtccgcgc gcccggcgcc gaccagcgcc ttcttgacct ggcgattgtg gtattcggcg
     4741 accgccaggc cggcgctggc cggcgccagc agcgcgacgc cgcgcgcctg gccgagcttg
     4801 agcgccgagg ccgggttgcg gttgacgaag gtctcctcga ccgcggcgct ggccggggca
     4861 taggtcagga tcacctgggc gagcccgcga tacaacgtgg ccagccgcgc cgccagcggc
     4921 gcgcgcgcgt cggtgcgcac cgtgccgcag gcgacgaagg caagcctcgt tccggcgcag
     4981 tcgagcaccc cccagccggt cgcgcgcagt cccgggtcga ggccgagcag cctcatcgcc
     5041 ccgcccctcg tcccgcctcg ccgcgcgcgc ccgccgccgc catgctcagg cgcccgcgct
     5101 cagccgctgc atgacctcgt cggggatctc gaagttggcc gacaccgatt ggacgtcgtc
     5161 gctgtcctcg agcgcctcga tcatcctgag cagggtcgcc gcctcgccct cgtcgacctc
     5221 gaccgtgttc tgcggcagcc actcgagccc ggcagagctc ggcgcgccga agcgctgggc
     5281 gagcgcctcg cggacgtcgt tgagccgctc gggccggcag gtgacgacgt ggcccgagct
     5341 gccgctgtcg acgtcgtcgg cgccggcctc gaccgcggcc tcgaacattg cgtcggcgct
     5401 ggcggcgccg gccggatagc ggatcgtgcc ggcgcggtgg aacatgaagt tgacgctgcc
     5461 ggactcgccg agcgagccgg cgtgcttggt gaacgccgag cgcaccgcgg ccgcggtgcg
     5521 gttgcggttg tcggtcagcg cctcgacgat cacggcgacg ccgccggggc cgtagccctc
     5581 gtagcggatc tcctcgaagg acgcctcgtc gccgccgccg gcgcctttct tgatcgcgcg
     5641 ctcgatgttg tccttgggca tgttggcgac gcgcgcggcg gcgatcgcgg cacgcaggcg
     5701 cggattggtc gccgggtcgg gcaggccggt cttggcggcg acggtcagct cgcgcgcgag
     5761 cttggcgaac atcttggcgc gcttcgcgtc ctgcgcgccc ttgcggtaca tgatgttctt
     5821 gaactgcgaa tggccggcca tcgagcctca gaccttccct tgtcgcggcg cgctgctcac
     5881 gggcgcgcgc cgagcccccg catcctgccc gtcggcgcct gtcaacatac cagatattga
     5941 ggtccgcaac aggcctacgc cccaatgaac cagtgcctaa agcatctcgg ctaacaaacc
     6001 attatggcga caatttgtcg ccgcgcgcgt ggccgcgcgc gcgctcacag cggccaccgc
     6061 ggcttgagcc tgccgccgca cgccagcggc ccgatgcggc gggcgaggcc ggtcgcctca
     6121 tcggtctcga cgaacaccgc gcacaaggtg cccgcgccgg tcgccggcgt gatccgcccg
     6181 accggcatct tggagcggaa cttctgcacc cacatcccct tgtcgctgcc gatcaccgag
     6241 tcgtagtcgc cgcacatgcc ggcgtcggtc tggaacccgg tgccgccggc cagcaccatg
     6301 gcatcgccgg tcggcacgtg ggtgtgggtg ccgaccacca ggctcgcgcg gccgtcgagg
     6361 aagtgcccga tcgcctgctt ctcgctcgag gtctcggcat gcacgtcgat gaccgcggcg
     6421 tcgatgtcgg cgccgagccg atggttggcg aacgcctggt cgaggcaggc gaacgggtcg
     6481 tcgagcgggt ccatgaacag ccggcccatg acgttgatca ccagcacccg cttgccgcgc
     6541 tgggtgttga acacgccggc gccctgtccg ggcgtggtcg ccggcgggta gttgcacggg
     6601 cgcagcaggc gcggctcgcg cggcagcagg gtgaccacct cgcgctggtc ccagacgtgg
     6661 ttgccggtgg taatcacgtc gacccccgcc tcgaacaggt cgtcggcgat cggggcggtg
     6721 atgccgaagc cgccggcggc gttctcgccg ttggcgacca cgaagtcgat ctcgagctcg
     6781 cgcttgagcc ggggcaggtg ctcggcgatc gccgcccgcc cggagctgcc gacgatgtcg
     6841 ccgcagaaca ggatcctcat cgcgccgccc cgatgtgaag cgcgccgcgc tcggtgacga
     6901 tccagtcgag cgccatgtcg tgcggctcgc gcggcaggcg ggatacctcc tgggcggcga
     6961 aggcgaggcc gaccgacagc cgcccgctgc cctcgagcgc cgccagggtc aggtcgtaat
     7021 agccggcgcc gaagccgagc cggtagccgt cgcggtcgaa ggcgagcagc ggcaccagca
     7081 ggcagtcggg gaccacgcgc ggacgatcgg gcgccggcac gctggtggcg aacacgccct
     7141 cggtcagcgc ctcccccggc gcccaggcgc gaaaggcgag cggcgtgccg ggccgctcgg
     7201 cgaccggcag gcacaacggg tggccacggg catggagggc aatcatcaac ggcctcgggt
     7261 cgacctcgtc ggcgaacggc caatatccgg acacgaccgc ggccggcgcc agcgcaacgg
     7321 cggcaaggac attgtcggca atccgcgccg cggcctcggc gcgcgcgccg gcgccgatgc
     7381 gcgcgcggcg ctcgaggcac tccgcacgca gccgcgcctt ctcggccgcc ggcgctagcg
     7441 gcacccggac gcggtgggcg ggaccgcctc gaccgttggc gttgtgtgcc actgtggcct
     7501 gcatgtgcag gtgggtgcca tataccgacg gcaggccgcc ggcagggaca gcccccaagt
     7561 ggagctatgt tggcccaggg acatgttcgc ctgcgacgaa tcgcgcagta cccgcccgcc
     7621 gctatacgta gagcgtctcc aggcgtccgg caatacggtc gagccgctcg gccataccgt
     7681 cgagggccgt ggcgaagtcg gttttgccgc cgccgacgcc gccgccgccc ggcccggtct
     7741 tgtcgtaggc atccgacagc tcgtcggcca ccgtcagcgc cgccagcacc atcaggtggg
     7801 tatcgccgac ctggccgagg ttgacgttgc cgcgcttgag caggttcctg accacggcgt
     7861 cgatatacgc cccgagctgg cgcaggtgct cctgctcgcc gtcctcgcac tggaccgcat
     7921 acgacctgcc gttgatctgc accttgactt ggggcatggc tcctgtcccg tttggcgcgg
     7981 ctcagccgcc gagcatgccg cgcacgcgcg cgatcgtctg gtcgaggcgg tcggtggcgg
     8041 tgtcggtcag cgccttgagg tcgtcatagc gctgctgcag cgccttgagc tccgcggcca
     8101 ggcggtcgcg gtcggcgcgc acgccggcaa gctcggccga ggcggcgcgc cggccggacc
     8161 cggcaccggc gctcgccgcg tgctcgaccg ccgcctccag ccggtcgagc gcggcctcga
     8221 tgcgcccgcg cgcctgttcg atggtcgcca tccgcccccg cgtcggtgtc cgcccggcgc
     8281 gcccgcaggg cttgcgcggg ctcgccggac ggccagcata tcggccgctc gccgcgaccg
     8341 tcaacgacgc caactcgccg gttgacggcg caccgccggg cggtatgttc gcccaagctc
     8401 gcgcgcgcct cgactcaggc ggcgcggggt tgcgccaaag cttcggaaaa cgcttgccca
     8461 tgaccgtacc caaggcatcg cccgcggcct cggacgtggt cgccgcgccg agccagcgcg
     8521 acctcgccaa cgccatccgc gcgctggcga tggactccgt ggaggcggcg aagtccggcc
     8581 atccgggcat gccgatgggc atggccgacg tggccacggt gctggtccat cggttcctcg
     8641 ccttcgatgc cgcggccccc gactggcccg accgcgaccg gctggtgctg tccgccgggc
     8701 acggctcgat gctgctctac gcgatcctgt acctgaccgg caccgccggc atgaccatcg
     8761 acgacatccg caacttccgc cagctcggca gccccgcggc gggccatccg gaatacggcc
     8821 acgcgcccgg catcgagacc accaccgggc cgctcggcca ggggcttgcc aacgcggtcg
     8881 gcatggcgct cgccgagcgc ctgatggcgg cgcgctacgg cgccgacctg gtcgaccatt
     8941 tcacctacgt catcgccagc gacggcgacc tgatggaagg catcagccac gaggcggcct
     9001 cgctcgccgg ccatctccgc ctcgccaagc tgatcgtgct gttcgacgac aaccacgtct
     9061 cgatcgacgg cccgaccgag cttgcggtct ccgacgacca gccggcgcgg ttccgcgcgc
     9121 tcggctggga cgtggcggcg tgcgatggcc acgacgcgga ggcggtggcg gcggcgatcg
     9181 cgcgcgcgcg ccggaccgac cggccgtcgc tgatcgcctg ccggaccacc atcggctacg
     9241 gcgcgccgac caagcagggc accgcggcga cccacggcgc gccgctcggt gcggacgagg
     9301 tcgcgctggc gcgcgcccgc ctcggctggc cgtcgccgcc gttcgaagtg ccggcggcgg
     9361 tactcggcgc gtggcgcacg gcgggcaggc gcggggcggc ggcgcgcgtt gcctggcagg
     9421 cgcgccttgc cgccgcgccc gcgcgcgcgc gcttcgagcg cgtgctcgcc ggcacccttc
     9481 ccgacggcct cgcgccggcg ctcgcggcgc tgaaagcgcg gctggcgagc gacgcgccca
     9541 ggatcgccac ccgcaaggcg tccgagatcg cgctcgaggt gatcaacgcc gaggtcgagt
     9601 gcacggtcgg cggctccgcc gacctgaccg gctcgaacaa cacccgcacc aagggcatgg
     9661 cggcggtcgc gccgggcgac ttcgccggcc gctacgtctt ctacggcgtg cgcgagcacg
     9721 ccatggctgc ggtgatgaac ggcatcgcgc tccacaaggg cctgatcccc tatggcggca
     9781 ccttcctgat cttttccgac tacgcgcgcc cggcgatgcg gctcgcggcg ctgatggggc
     9841 tcagggtgat ctacgtgctg acccacgatt cgatcggcct cggcgaggac ggcccgaccc
     9901 accagccggt cgagcacctg gcgagcctgc gcgcgatgcc caacctgtgg gtgttccgcc
     9961 cggcggacgc catcgagtgc gcggagtgct gggagctcgc gctcgcgcgc agctcaggtc
    10021 cgtcggtgat cgcattgacc cgccagggcg tgccgtgcgt gcgcgcgggc gccgggcccg
    10081 ccaaccgctc ggccgaaggc gcctacgaga tcgccgcggc cgaggggcgg gcgcaggcga
    10141 ccatcctcgc cactggctcc gaggtggcgg tcgcgctcgc cgcgcgcgcg gtcctggagg
    10201 gcgagggcat tgccacccgg gtggtgtcga tgccgtgctg ggagctgttc gccgagcagc
    10261 ccgcggccaa gcaggccgag gtgctcggcc ccggcacggt gcgcgtcgcg gtcgaggcgg
    10321 cgtcgcccca gggctgggcg cggtggggcg tcgatgagac aacaatgatt gccatgcgaa
    10381 cattcggcgc ctcggcgccc gccgagcgtc tgttcgcgca tttcggcatc accgccgagg
    10441 cggtggcgga ggcggtccgg cgcaagctct gagcgcggcg cggacggcct gcggaaagga
    10501 ggcggacgtg gcgattcgga tcgggatcaa cggcttcggg cggatcggcc ggctggtgat
    10561 gcgcgccgcg gccgaggcgg ggcggaacga cgtccaggtg gtcggcatca acgacctcgg
    10621 gccggtcgag accaacgcgc acctgttccg ccacgactcg gtgcacgggc ggttcgcggg
    10681 cgaggtcaag gtcacgggcg acgccatcga catcggcgca ggcgcaatca aggtcaccgc
    10741 cctgcgcgag ccggcgaagc tgccgtggcg cgagctcggc gtcgacgtgg cgctcgaatg
    10801 caccggccgc ttcaccgacc gcgccgcggc ggcggcgcat ctggaggccg gcgcgcgcaa
    10861 ggtgctggtc tcggcgccgg ccaagggcgc ggacctgacg gtggtgttcg gggtcaacca
    10921 cgaccgtctc acgcccgagc accaggtggt gtcgaatgcg tcgtgcacca ccaattgtct
    10981 cgcgccggtg gccaaggtgc tcaacgacgc ggtcggcatc gagcgcggct tcatgaccac
    11041 catccatgcc tacaccggcg accagccggt gctggacacc ctgcacaagg acctgcaccg
    11101 ggcgcgcgcc gcggcgatgt cgatgatccc gacctcgacc ggcgcggcgc gggcggtcgg
    11161 cctggtgctg cccgagctcg ccggcaagct cgacggcacc gcgatccggg tgccgacgcc
    11221 gaacgtgtcc ctgatcgacc tcaccttcgc cgccgcgcgc aagaccagcg tcgatgagat
    11281 caacgacgcg gcgcgaaagg cggcggcggg gccgatgcgc ggcgtgctcg cgacctgcga
    11341 tgcgccgctg gtgtcgatgg acttcaacca cgacccggcg agctcgacct tcgacctcgg
    11401 ccagacccag gtgatcgacg gccgcttcgt gcgcgtgctc gcctggtacg acaacgagtg
    11461 gggcttctcc aaccggatga acgacgtcgc cgcggtcctg ggcgcgctgt aggaccggcg
    11521 atcgtggccg catgcatggc ggacctcatc cttcgagacg cccgctgcgc gggctcctca
    11581 ggatgaggtt tgtcgtaagt gagagcctca tcctgaggag ggccaggggc ccgtctcgaa
    11641 ggatgaggcg ggcggccgca attcgtgacg ccgggcgcta ggcgggcgtg gaaaagctga
    11701 gcctgctacg cgatctcgac gtcggcggcc ggcgggtgct gctgcgcctc gacctcaacg
    11761 taccggtcga cgaccaccac cggatcaccg acgacacccg catccggcgc gcgctgccga
    11821 cgctccgcga gctgtgcgag cgcggcgcgc gcaccgtcat cctgtcgcat ttcggccggc
    11881 ccaagggccg acgcgtggag gagatgtcgc tgggcttcct gtcggcgccg ctgagcgacg
    11941 cgctcgcgcg cgacgtcgcg gtggcgagcg actgcatcgg caagggcccc gaggcggtcg
    12001 ccgccgcact cgagccgggc gcggtggcgc tgttcgagaa cctccgcttc cacgccggcg
    12061 aggaggcgaa cgacgccggc ttcgcggacg cgctcgcgcg ctcgggcgac gcctatgtca
    12121 acgacgcgtt ctcggtctgc catcgcgcgc atgcctcggt ggtcgggctg ccggcgcgcc
    12181 tcgcgcacgc ggccgggcag gcgctcgagg ccgagctcga ggcgctcgac gccgcgcttg
    12241 gccacccggc gcggccgctg ctcgcgatcg tcggcggcgc caaggtgtcg accaagatcg
    12301 gcgtgctcgc aaacctgatc ggcaaggtcg acctgctcgc catcggcggc gccatggcca
    12361 acacctttct cgccgtacag ggccacggcg tcggcgcctc gctgtgcgag cccgagctcg
    12421 cggatgcggc acgccggatc atggccgatg cgcaggccgc ggggtgcgag atcatcctgc
    12481 cggtcgacgc ggtggtggcc gatgcgctcc gcgccggtgc cgagcccgcc atcgtcgcgg
    12541 tcgacgcggt gccggccggc aagatgatcc tcgacatcgg gccggcctcg gcggacgcca
    12601 tcggcaaccg catcccggac tgccgcacct tgttgtggaa cggcccggtc ggggcgttcg
    12661 aggtccaccc gttcgatacc tcgaccgtgc tgctcgcgcg caccgccgcg accatgaccc
    12721 gggcgggcgc gctggtcagc gtcgccggcg gcggcgatac cgtggcggca ctcgcccatg
    12781 ccggcgccga ggccggcttc acctatgtct cggccgcagg cggcgcgttc ctcgaatggc
    12841 tcgagggcaa gacgcttccc ggcgtcgccg cgctgatggc ggactagcat ggtcatgggg
    12901 tggtgatcgt ccacgggccg gaccacatcg aggcctgctt cgcggcggcc gagcagtccg
    12961 ggcgcgcgct gagcgcactg gtcgcggcgt gggcgggcgc ggcttacggg ccgctctacc
    13021 tggccgcggc ggtcgctgcc gcgcgcgccc gccatccgcg ggccgaggtg cggatcgcgc
    13081 tcgactgcgc cgacgacgcc ggccgggcca tggcggcgct gcgtagcggc tggcaacggc
    13141 tcgccttcag cggccgggcg gacgtgcgcg cgcgcatcgc cggcatggcc gaagcgcaag
    13201 gcgcgatgct gatcgagtcg cacacggggc cggtgctcga cctcgccggc gaggccgcgc
    13261 ccgaggccgc gctcgcgcgc cggctggccg attgcaaatg acccgaccct gggctaagca
    13321 ccggtcttgc gcggccggca cacgcaggca acggaggcta cgatgagagt gacccagcgg
    13381 gtgcgcagga ttctcgccaa ctacgagagc gacaaccccg gcaccaaggc caatctcgcg
    13441 cgcatcctga tgcacggccg gctggcgggc agcggccggc tggtgatcct gccggtcgac
    13501 cagggcttcg agcacggccc ggcgcgcagc ttcgcgccca atccgccggc ttacgatccg
    13561 cactaccatt tccagctcgc gatcgacgcc gggctcagcg cctacgccgc gccgctcggc
    13621 atgatcgagg cgggcgcgga cagctttgcc ggcgcgatcc cgaccatcct caagctcaac
    13681 agcgccaaca gcctcgcggt gaccaaggac caggcggtga caggctcggt cgcggacgcg
    13741 ctcaggctgg gctgcgcggc gatcggcttc accctctatc ccggctccga ggaccagtac
    13801 gagatgatcg aggagctgcg cgagctggcc gaggaggcca aggcggcggg tctcgcggtg
    13861 gtggtctggt cgtacccgcg cggcggcaag ctgtcgaagg agggcgagac cgcaatcgac
    13921 gtgaccgcct atgcggcgca gctcgcggcg ctgctcggcg cccacatcat caaggtcaag
    13981 ccgccgaccg agcacatcga gcaggacgag gcgcgcaagg tctaccaagc gcagggcatc
    14041 gacgtgtcgt ctttgcgcgc gcgcatcgcc cacgtcgtgc agtcggcgtt caacggccgc
    14101 cgcctggtcg tgttctcggg cggcgcggcc aagggcaccg agggcctgct cgacgagatc
    14161 cgcgagatcc gcgacggcgg cgccaccggc tcgatcatcg gccgcaacag cttccagcgc
    14221 tcgcgcaagg acgcgctcgc tttgctcgat acgatcgtca ggatctatca gggcaaggcg
    14281 tgagccgcga acgctgccgg ctgtacctga tcacgccacc gggtctcggc cacgaccggg
    14341 cggcgctcga cgcgttcgcc atccaactcg cgggcgcgct ggcggcgggc gacgtcgcct
    14401 gcgtccagct ccgcctcaag ggcgcggacg atgccgccgt gctgcgcgcg gccgcggcgc
    14461 tgatgccgcc ctgtcatcgg cgcgaggtcg cagtcctggt caacgaccgt gccgacctcg
    14521 ccgcgcgcgc cggcgccgat ggcgtccata tcggtgccga ggacggcggc tacgcgcgtg
    14581 cgcgcgccct ggtcggcgag gggcgcatcg tcggcgtgag ctgctacgat tcgcgccatc
    14641 tggcgctgga ggcggccgag gccggcgccg actacgtcgc cttcggcgcg ttcttcccga
    14701 ccgcgaccaa gcagccgccg gcgcaggccg gcctcgattt gttgcgctgg tggcaggaga
    14761 tgatcaccgt gccctgcgtc gccatcggcg gcatcaccgc cgacaactgc gccccgctgg
    14821 tcgaggccgg cgccgacttc ctggccgtca tctccggcgt ctgggattac ccggacggcc
    14881 cggcggcggc ggtgcgcgcc ttcaacgccg agatcgaccg ggcgatggcg cgcgccgaca
    14941 tcgattcggc ctgagccggc atgtcacccg atctctccca atagcggcac cgcatccagg
    15001 aaccagtagg acaagaccga cagctggccg gtgagcatgg cgaggccgat cgccaccatc
    15061 acgccacccg cggcaagctg cagcaccatt cccgcgcggc gcgctgaccg tagccagtag
    15121 acgattcgct cgaccagcag tgcggagatc aggaacggga cgccgagacc cagggaatag
    15181 aaaccgagca gggtcacgcc tcccgagacc gtcgccgagg tcgcgccgac agtgagaatc
    15241 gcgccgagga tcgggccgat gcacggcgtc cagccgaagg cgaaggcgag acccagcaga
    15301 taggcggact cgggccggcc gccgacgacc ggcaaggtca ggcgcaggtc cctgagcagc
    15361 caaaccggct tgatcaggcc aagagtgacg atgccgaaca gggcgacgat ggcgcccccg
    15421 accagcgtca gctgctcgcg atatgcgagc aggagctgcc cagccgcggt cgcgctggcg
    15481 ccgagcagca cgaacacggt cgagaagccg agcacgaagg caaaggcgag aacgaccacc
    15541 gtgagtcgaa accgtgcgtc gcgcggctcg tccgggtcga ccaccgagcg accggctatg
    15601 taggacagat agcccggcac cagcggcagc acgcacggcg acaggaacga gacaagaccg
    15661 gcgccagccg cggtcagcag gcctatgttg ctggccggat ccatggcggt cgccgggctg
    15721 gcgacatcag ccctctacgt cactggaacc gcgtgccttg gcgatcgagc cctggagcgt
    15781 gccgaggtcg gtcgccccgc ggatgatgtc ctggccgacg acgaagccgg gcgtaccggt
    15841 gatccgcaga tccagcgcca gcctcatgtt gcgctcgatc gccgccgcga tcgccggatc
    15901 ctccatgtcg cgcctgagtg cctcgaggtc caacccgatc tcgttcgcga tgtcggtgac
    15961 ggcgtcgtcg tccgctcgct ttgcaaccat catggccgtg tggaacgcga catacttgcc
    16021 ctgcctgtgg gcggccagcg ccgccttcgc ggccgtgacc gagttggccc ccaggatcgg
    16081 gaactccttg tagacgacgc gcagctcgcc gtcggcggcc acggcctcct ggaccaccgg
    16141 cgcgactcgg cggcagtaag ggcagttgta gtcgaagaac tcgaccagcg tgacgtcgcc
    16201 cgccggattg ccgcccaccg gactgtcggg atcgcgcaga atctcgccgg cccgctcgtc
    16261 cagggcggtg atcgcccggg tctcctgctc cgcctgctcg cggtcgcgga gcgcggtgac
    16321 ggattccagg atgacctgcg ggttgcgcat caggtagtcg cgaacacggc ggtcgaactc
    16381 gtcctcgggc acgccggcga cgtccgttcg ttcgccgccg gcgcgctcga agcccggatc
    16441 ggcccacagc cagaatgccg ccccggccgc cagcaggacc gccagcaggc tgacggggac
    16501 aagtgtcgtc gcaacgcggt acttgggcat ctcgacaatc ctcgcatccg ttcaggattt
    16561 ggccgaccgc gaccatcgac tttccaattg gccgaacagg tcccacagcc agtcgaggac
    16621 tccggccggc tcgtcatggc cgtgcgggtt tgtgatatcg ttgacgagcg cgttcaggaa
    16681 cacgaccagg ctggtcggct cgaacttcaa gccatgaaag ttggcgcgcc aacggccacg
    16741 gctgtcgatg acatgggtca ccaccccgtg gatctgatag ccatcctcgg tcttagtgaa
    16801 cttgtggccg aactgttccg ccaggttgcg cgtcacgtcc tcgggctgtt cgggaccggc
    16861 cgtgagatag agccagttga ccggatcgag cccgtgcgcc acgccgaaat ctcgcaagac
    16921 gtcgggcgta tcgttcccgg gatcggtggt gatggtcacg aaccggacct ggtccgtcat
    16981 cggcgtctgg ttgaccatcg cctggatgtc ggcgaggcgg tcggcatgga gcgggcacac
    17041 gtcggggcaa ctcgtgtaga tgaagtgcag cacgacgacc ttgccgagaa ggtctgccgg
    17101 agagacgcgc tcgccgcccg agccgagcag cgtgaaatcc ggcgtcggct tatcgaccgg
    17161 ctggaaatat tgctcccggt cgcgcagctc gccctcgagc tcgtcgagcg aatgcgcagc
    17221 cgccggtgcc acggccgcga gcgacaggac gagaaggatc ggcagccgcc tgaggagccg
    17281 catcgttcga gagctccgcc tcgccgcact ttctgcccac cttgtggact ggcgggtcag
    17341 tacttcatcg cttcgtgtcc ggggccgtgg gaccatcacg gcgccactgc tcgttcggcc
    17401 ggccagcgcc acgatcacct gtcgtgccct ccatcatctg ggcgcattgg tccgtcatcc
    17461 ggcccatctg ccccatcatg ttcatcattc ccatcatccc gccttggccc atcatgccgc
    17521 cctgtcccat catgccgggg cccatcatcg atccgcgtga gtcgtctgag tcgtgggcat
    17581 agagggccgg cgcggcggcg aggccgccgg cgagaacggc ggcgagggcg agagtccttg
    17641 tggacttatg catcgggatt tctccttgtc tcttggggtt gggcgtcgat cgcgacgccg
    17701 ggtgcagcga agacgacggg ctcgccaaca aggccgtcgc cgtttgcaac ttgccattcg
    17761 acgacggcgg tctcggcggg cgtgacccgg ctggtcgccg ttgcaccggg ctcgacgggg
    17821 cgcgcggtgc ctgccgcaca cgagcgcccg gccatcttgt tcatgcacag gccgagggcg
    17881 cacatcgcca ggcacggcaa cacgccgagc aacagcggcg cgagcccgat cgccaccagc
    17941 cagctccagt tggcggcgag gccgatggcg agcgccgcgc ccgcagcgat catgatgccg
    18001 cgacggccgc tgagatgacg tcggaggccg ttgagcacgt cgcgagccgg cccagggctg
    18061 gagatcgcgg gtaccctcaa taacgtcatg atggaggtcc ttccttgtga ctgagaggac
    18121 gtagtgcttc cggtaacggt aaggtcaagg cttgggcgta gcggacgtcc gcgccgcttg
    18181 gcggtctcgt ggcggcggca cgtcgtgggc gcgcaagcgc gggatcgctg cgctcgggcc
    18241 cggcgctcat ctggcgcgcg acgacatcgc cgaggaaggc gatcatgtcc ggcgcgtccc
    18301 actcggccgg gccgaccagg cggccgagct cgcgcccctc gcggtcgatc aggagcgtcg
    18361 tcggcagtcc gacgacgttc agcgcgggtg ctgcctgccc cgagtcgtcg atgtggagcg
    18421 caagtccgga cacgccgacc tcgtcgtaga actcgcgcac gacctccgcc ccgcctcggt
    18481 cgatcgacaa agcgatcacc tcgaagtccg ggccgccaag ctccgcctgg agccggtcga
    18541 ggctgggtat ctcgcgccgg cacgggacgc accacgtcgc ccagatgttg agcagcacga
    18601 ccttcccccg gaagtccgcg agcgatcggg cgccgccgtc ggcgccggcg aacccgacgt
    18661 tcggaacggt acccggacga tcgagcaacg ggatgaccgc ggcgcccggc gagggggacg
    18721 gggtctcggc cagcgacggc gtggcctcca agcctttgtg ctcggcgcga aaggtgagga
    18781 acgaatttcc cgcgtagccg agaagcaagg cagaagcggc ggcgatggcg acggtcgtcg
    18841 ctacaatcgc gatcttgcgt acggacggcg gcgcggactt aaggatcgtc agacgcattt
    18901 gcacgtctcc cgtcctcgcg cagccgcgcg accagcggca ggatcgtccg gtcgaggtcc
    18961 ttctgcgtca ccgggccgat atgcttgtgc gcgatcccgc cgtcgggaga gatcacgaag
    19021 gtctcgggca cgccatagac tccgaaatcg attccgacgc gcccatcgat gtcggcgccg
    19081 gtgcgggtat agggatcacc caggctatcg agccagcccg ctgcgtcggc cggccggtcc
    19141 ttgtagttga gcccgtgaat cgggacgacg ccctcgtccg cgagccgcat gaacaagggg
    19201 tgctcggcgc ggcacgccgt gcaccacgac gcgaacacgt tgaccagcga aacctcgccg
    19261 accaggtcgc cgctcgccag gccgagctcg cgcccctgca ccggcggcag gctgaattca
    19321 ggcaccggct tgccgacgag aaccgatgga atctggctcg gatcgcggcc gagcccccag
    19381 gcgaatacga cggccaggcc gaaaaacgcc aacagcgg
//
LOCUS       DASWHU010000141         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3027436, whole genome shotgun sequence.
ACCESSION   DASWHU010000141 DASWHU010000000
VERSION     DASWHU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027436"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGB88_05300"
     CDS             <1..192
                     /locus_tag="VGB88_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX9646891.1"
                     /translation="SESFLDTYLAAAGADALDSVGCYRIEGLGAQLFARIDGSQFAIM
                     GLPLLPLLGFLREHQVLGE"
     gene            189..1034
                     /locus_tag="VGB88_05305"
     CDS             189..1034
                     /locus_tag="VGB88_05305"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9646892.1"
                     /translation="MILSGGARIAGVIGWPVAHSRSPRLHGYWLDRYRIDGAYVPLAV
                     PPARLAQAIAALPALGFVGANVTVPHKAAALAAVDEADALARRIGAVNTISVRPDASL
                     FGTNTDGYGLIQNLDQGARDWPRDRPAAVLGAGGAARAVIVALADAGVPEVRLCNRTR
                     ARAEALADEIEAPIAVAGWEVRAGALDGAGLLVNTTTLGMAGAAPLTIDLGPLPADAV
                     VADLVYVPLVTPLLAQAAARGLRVVDGLGMLLHQARPGFRMWFGRDPEVSAELRRFVL
                     QDLAR"
     gene            1043..1711
                     /gene="coaE"
                     /locus_tag="VGB88_05310"
     CDS             1043..1711
                     /gene="coaE"
                     /locus_tag="VGB88_05310"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386890.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9646893.1"
                     /translation="MRRPVVVGLTGGIAMGKSVAARALRRLGVPLFDADAAVHRLLAP
                     GAKAHRPVAAAFPDALVEGRIDRARLGARVFGDDAALARLEAIIHPLVGEARQAFLRR
                     ARALGRPVAVLDVPLLFETGGEARCDYVAVVSAPRFVQRRRALARPGMTESKLAAILA
                     RQMSDGDKRRRADFVIPTGLGRRATLCAIRRMLTMVADSQPRSPPGLTRGSPRRHARG
                     RARH"
     gene            1686..2360
                     /gene="dnaQ"
                     /locus_tag="VGB88_05315"
     CDS             1686..2360
                     /gene="dnaQ"
                     /locus_tag="VGB88_05315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX9646894.1"
                     /translation="MREVVLDTETTGLDPASGHRVVEIGCLELVNRIATGRTFHHYLD
                     PERDIPPEATEIHGITAATVAGKPVFADVAGDLLAFLADSALVIHNAAFDLGFLNAEL
                     ARIERPQIPARRAVDTVQIARRKFPGAPASLDALCRRFAIDNSARTLHGALLDAELLA
                     EVYVELIGGRQAGLELVAGAAQAEALAPADALPVRPPRAHAPSEAERQAHEAFIDTLP
                     NALWRL"
     gene            complement(2446..2808)
                     /locus_tag="VGB88_05320"
     CDS             complement(2446..2808)
                     /locus_tag="VGB88_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646895.1"
                     /translation="MLKPLDVIWIFDGCIRPPGPKMVVCIEPSLGLFFRINTEPKWQT
                     PVELRKSDHPFLDWDSNLECGEPLEFDDYVIEESLRDRGVIGRVLPSLAPQIFEAVRN
                     ARTVTAADKEAIRIALGC"
     gene            complement(2814..3290)
                     /locus_tag="VGB88_05325"
     CDS             complement(2814..3290)
                     /locus_tag="VGB88_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HEX9646896.1"
                     /translation="MVDQLAGVDLHTLLKACYFADKKHFNEHGRPIFGATYRAMKFGP
                     VPMEIYEMAKGEALWLAELEADWYPWRLEGFRLRPTGNQAPDLEVLSQSDLEALQHGF
                     QKSRTLTFNARTAATHGPDWQAAKLGLMRYEDMLEDSADKAKRVAYLREAAPYIRL"
     gene            complement(3564..4106)
                     /gene="secB"
                     /locus_tag="VGB88_05330"
     CDS             complement(3564..4106)
                     /gene="secB"
                     /locus_tag="VGB88_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX9646897.1"
                     /translation="MADEAGSGKGAGQPQRAAAGDGAQPQLAIRAQYVKDLSFENPQA
                     PQALQRREPPRVELQVQVGGRALGEGNHEVELHLTARASHGEELAFLAELVYAGVFFL
                     ANIPPEQMRPVLMVECPRLLFPFARRVVADCTRDGGFPPLMLEPIDFAALYRRSEAQR
                     RAGDGGATGDGAGKSADAKA"
     gene            complement(4152..4712)
                     /locus_tag="VGB88_05335"
     CDS             complement(4152..4712)
                     /locus_tag="VGB88_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9646898.1"
                     /translation="MILMRHGQSEFNVVYGATRTDPGIRDPRLTALGHSQVADAAAGL
                     RAAGVAIRRVVASPYTRALESARILAAALAVPVAVEPLVRERFAWTCDIGTPLSELAP
                     RWPGVSFPDMAERWWPEREEHDHEVYGRATAFRRAMAGDPVHAETLVVSHWWFIRAFS
                     GAELANGALVRVRLAETVEVVTDSHP"
     gene            complement(4709..5206)
                     /locus_tag="VGB88_05340"
     CDS             complement(4709..5206)
                     /locus_tag="VGB88_05340"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX9646899.1"
                     /translation="MTVMIILLMVALPLVEIAGFVVVGERIGVLATLGLTVAGAALGL
                     AVLSRGPDRLAERARRAMARDQPPVAEALDGLAVALAGMLLIVPGFVTDLAGLLLLVP
                     PVRRAAIAAGLRTLARAADGEVVIEGEWRDVTDLQSAEADFRSAKADAPAGRRLDRDR
                     SGRGQ"
     gene            5405..6085
                     /locus_tag="VGB88_05345"
     CDS             5405..6085
                     /locus_tag="VGB88_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX9646900.1"
                     /translation="MQGFHFLDILVFAMIAAFLVLRLHRVLGRRTGHQRPPEPAPREA
                     EKGNERVVQLPDRSRARQASAEDAPPPPPGATRGAAGLTQVKIADPSFDEGQFLDGAR
                     AAFEMILGAFAGGDKRQLRQLLAPTVFEGFAAEIDRREKAGEQLETTLVSFILADIVD
                     ARMDGRKARVTVKFMTEQVNVLRDAKGEVVEGDPATVAKITDVWTFERDTRARDPNWQ
                     LAETRSDD"
BASE COUNT          867 a         2204 c         2127 g          901 t
ORIGIN      
        1 tcggagtcgt tcctcgacac ctatctcgcc gccgccggcg ccgatgcgct cgactcggtg
       61 ggctgctacc ggatcgaggg cctcggcgcc cagctgttcg cccgcatcga cggcagccag
      121 ttcgcgatca tgggcctgcc gctgctgccc ctgctcgggt tcctgcgcga gcaccaggtg
      181 ctcggcgaat gatcttgagc ggcggcgcgc gcatcgccgg ggtaatcggc tggccggtgg
      241 cgcattcgcg ttcgccgcgc ttgcacggct actggttgga ccgctatcgg atcgatggcg
      301 cctatgtgcc gctcgccgta ccgccggcgc ggcttgccca ggcgatcgcg gcgctgccgg
      361 cgctcggctt cgtcggtgcc aacgtcaccg tgccgcacaa ggcggcggcg ctggcggcgg
      421 tcgacgaggc cgacgcgctc gcgcgccgga tcggcgcggt caacaccatc tcggtgcgcc
      481 ccgatgcgag cctgttcggc accaacaccg acggctacgg cttgatccag aaccttgatc
      541 agggcgcgcg cgactggccg cgcgaccgcc ccgcggcggt gctcggcgct ggcggtgccg
      601 cgcgcgcggt gatcgtcgcg ctcgccgacg cgggggtgcc cgaggtccgg ctgtgcaacc
      661 gcacgcgcgc gcgcgcggag gcgctcgccg acgagatcga ggcgccgatc gcggtggccg
      721 gctgggaggt gcgcgcgggc gcgctcgacg gcgccgggct cctggtcaac accaccaccc
      781 tcggcatggc cggcgccgcg ccgctcacga ttgatctcgg cccgctgccg gcggacgcgg
      841 tggtcgccga cctcgtctac gtgccgctgg tcacgccctt gctcgcccag gccgcggcgc
      901 gcggcctgcg cgtcgtcgac ggcctcggca tgctgctgca tcaggcgcgc ccgggcttcc
      961 gcatgtggtt cgggcgcgac cccgaggtca gcgcggagct gcgccgcttc gtgctccaag
     1021 acctcgcgcg gtgagcaggc gcatgcggcg gccggtggtg gtcgggctca ccggcggcat
     1081 cgccatgggc aagagcgtgg cggcgcgcgc gctgcgccgg ctcggcgtgc cgctgttcga
     1141 cgccgacgcc gcggtgcacc ggctgctcgc gccgggggcc aaggcgcacc ggccggtcgc
     1201 agcggccttc cccgacgcgc tggtcgaggg ccgcatcgac cgcgcccgtc tcggcgcgcg
     1261 ggtgttcggc gacgacgcgg cgctcgcccg cctggaagcg atcatccatc cgctggtcgg
     1321 cgaggcgcgc caggcgttcc tgcgccgggc gcgcgcgctc ggccggccgg tcgccgtgct
     1381 cgacgtgccg ctcctgttcg agaccggcgg cgaggcgcgc tgcgactacg tcgccgtggt
     1441 cagcgcgccg cgcttcgtcc agcgtcggcg cgcgctggcg cgcccgggca tgaccgagag
     1501 caagctcgct gcgatcctcg cccgccagat gagcgatggc gacaagcgcc ggcgcgcgga
     1561 cttcgtgatt cccaccgggc tcggccggcg cgccaccttg tgcgcgatcc ggcgcatgct
     1621 cacaatggtc gccgattcgc aaccccgctc acccccgggt ttaacccggg ggtcaccgag
     1681 gcgccatgcg cgaggtcgtg ctcgacactg agaccaccgg cctcgacccg gcctcgggcc
     1741 accgggtggt cgagatcggc tgcctcgagc tggtcaaccg gatcgccacc gggcgcacct
     1801 tccaccacta tctcgacccc gagcgcgaca tcccgccgga agcgaccgag attcacggta
     1861 tcaccgcggc gacggtcgcc ggcaagccgg tgttcgccga cgtcgccggc gatctgctcg
     1921 ccttcctcgc cgactcggcg ctggtgatcc acaacgcggc gttcgacctg ggcttcctca
     1981 atgccgagct cgcgcgcatc gagcgcccgc agatcccggc gcggcgcgcg gtcgatacgg
     2041 tccagatcgc gcgccgcaag tttcccggcg cgccggcgag cctggatgcg ctgtgccggc
     2101 gcttcgccat cgacaactcg gcgcgcacgc tccacggcgc gctgctcgac gccgagctcc
     2161 tggccgaggt ctatgtcgag ctgatcggcg gccgccaggc ggggctcgag ctggtcgcgg
     2221 gcgcggcgca ggccgaggcg cttgcgcccg cagacgcgct gccggtgcgc ccgccgcgcg
     2281 cgcacgcccc gagcgaggcc gagcgccaag cgcacgaggc gttcatcgac accttgccga
     2341 acgcgctctg gcggttgtag caggagggta actgccgagg tcgtttcctc gccggctgga
     2401 tgtgacgagg gtggttaagc aaattcaact ttggacggct ccgcgtcagc accccaatgc
     2461 gatacgaatg gcctccttgt ccgccgcggt gactgttcgc gcgttccgga ccgcctcaaa
     2521 gatctgtgga gcaaggctcg gcaaaacgcg tccaatgacg cctctgtcgc ggagcgactc
     2581 ctcgatgacg tagtcgtcga actcaagcgg ctcgccgcat tccagattgc tgtcccagtc
     2641 caggaaagga tggtcactct tacgtaattc gaccggagtt tgccacttcg gctcggtatt
     2701 gattcgaaaa aagagaccca aactgggctc tatgcaaacg accattttgg gtcctggtgg
     2761 ccgtatacag ccgtcgaaaa tccagatcac atcgagcggc ttcagcaact tgcctaaagc
     2821 cgaatgtacg gggccgcctc gcgcagataa gcgacgcgct tggccttgtc cgccgagtcc
     2881 tccagcatat cctcgtaacg catcagtccc agtttggccg cctgccaatc cggtccgtgc
     2941 gttgccgcgg tgcgcgcgtt gaacgtgagc gttcgggact tctgaaaccc gtgctgaagc
     3001 gcttccaagt cggactgcga aaggacctcc aggtcgggtg cttggttacc agtcggacgt
     3061 aagcggaagc cctccaatcg ccacgggtac cagtcggctt ccagttcggc cagccacaaa
     3121 gcctcgccct tggccatttc atagatttcc atagggaccg gcccaaactt cattgcacgg
     3181 taggtggcgc cgaaaatcgg ccttccgtgt tcgttgaagt gcttcttgtc ggcaaaataa
     3241 caggctttca gaagcgtatg cagatcaacc cccgcaagtt ggtcgaccat ccaatgtatt
     3301 gccgcgcgac ccttcgcggg cgaaaacctg aatctgatgg atttcatttc gccaggttcg
     3361 taccgcttct acgagattca cgcgaaggtg atcaatatat ctcagttaac cttgagaatc
     3421 aacgagttaa gtcaatttag caacgccgtc cccgacaacg ggttagcgat ttggcggtgg
     3481 attggtgtcg tccacctcga atggacgaac caatcgtgga gcctatggtc gctgctgcgg
     3541 ccgagcgccg gccgcttgcc gcctcaggcc ttggcgtcgg ccgacttgcc ggcgccgtcg
     3601 ccggtggcgc cgccgtcgcc ggcccggcgc tgcgcctcgg agcggcggta gagggcggcg
     3661 aagtcgatcg gctcgagcat cagcggcggg aagccgccgt cgcgggtgca gtcggcgacc
     3721 acgcggcggg cgaacgggaa caacagccgc gggcactcga ccatcagcac cgggcgcatc
     3781 tgctcaggcg gaatgttggc caggaagaac acccccgcat agaccagctc ggccaggaac
     3841 gcgagctcct cgccgtggct ggcgcgggcg gtcaggtgca gctcgacctc gtggttgccc
     3901 tcgccgagcg cgcgcccgcc gacctggacc tggagctcga cccgcggcgg ctcgcggcgc
     3961 tgcagcgcct gcggcgcctg cgggttctcg aacgacaggt ccttgacgta ctgggcgcgg
     4021 atcgcgagct gcggctgggc gccgtcaccg gccgccgcgc gctgtggctg tccggcgccc
     4081 ttgccgctgc ccgcctcgtc cgccattccc gtgctcgcct gtcgcgccgc cctcgcccgc
     4141 gcaggtggct atcacggatg ggaatcggtg acaacctcga ccgtctcggc caggcgcacg
     4201 cgcaccagcg cgccgttggc gagctcggcg ccggagaagg cgcggatgaa ccaccagtgg
     4261 ctcaccacca gcgtctcggc atgcaccggg tccccggcca tggcgcggcg gaatgccgtc
     4321 gcccggccat agacctcgtg gtcgtgctcc tcgcgctcgg gccaccagcg ctcggccatg
     4381 tccgggaagc tcaccccggg ccagcgcggg gcgagctcgg acagcggcgt gccgatgtcg
     4441 caggtccagg cgaagcgctc gcgcaccagc ggctccaccg ccaccggcac cgccagcgcc
     4501 gcggcgagga tgcgcgcgga ttcgagcgcg cgggtgtacg ggctcgccac cacgcgcctg
     4561 atcgcgacgc cggcggcacg cagcccggcg gcggcatcgg ccacctggct gtggccgagc
     4621 gcggtcagcc tggggtcgcg gatgccgggg tcggtgcggg tcgcgccgta gacgacgttg
     4681 aactcggact ggccgtggcg catcaggatc attgcccgcg cccgctccgg tcccggtcca
     4741 gtcgccgccc ggccggcgcg tcggccttcg ccgaccgaaa gtcggcttcg gccgactgaa
     4801 ggtcggtgac gtcgcgccac tcgccctcga tcaccacctc gccgtcggcg gcgcgtgcga
     4861 gcgtccgcag gccggccgcg atcgccgccc ggcgcaccgg cggcaccagc agcagcaggc
     4921 cggccaggtc ggtgacgaag ccgggcacga tcagcagcat cccggcgagc gcgaccgcca
     4981 ggccgtcgag cgcctcggcg accggcggct ggtcgcgcgc catcgcccgg cgtgcgcgct
     5041 cggccagccg gtcgggcccg cggctcagca cggcgaggcc gagcgccgcg ccggcgaccg
     5101 tgaggccaag ggtggcgagc acgccgatcc gctcgccgac gaccacgaat ccggcgatct
     5161 cgaccagcgg caacgccacc atcaacagga tgatcatcac ggtcatgctt gttccggcgc
     5221 cgccctgatc ccatgtggga acctgagccc ggtttcgcca ccgtctggac ccggcgcggt
     5281 gaacgcacta cagtccgagt ccaaccgggt ggcgcccagg cgggcgccgg gcgcgactct
     5341 agggtgggaa gccggggggc gcggacccga attcggcggg gcggcccgtc gcggcgaaca
     5401 agacatgcag ggatttcatt tcctcgacat cctcgtcttc gcgatgatcg cagcgttcct
     5461 ggtgctgcgc ctgcatcgcg tgctcggccg caggaccggt caccagcgcc cgcccgagcc
     5521 cgcgccgcgc gaggcggaga agggcaacga gcgggtggtc cagctgcccg accgcagccg
     5581 cgcccgccag gcgagcgccg aggacgcgcc gccgccgccg ccgggcgcca cgcgcggcgc
     5641 cgccggcctc acccaggtca agatcgccga cccctcgttc gacgagggcc agttcctcga
     5701 cggcgcgcgc gccgccttcg agatgatcct cggcgccttc gccggcggcg acaagcgcca
     5761 gctcaggcag ctgctggcgc cgacggtgtt cgagggcttc gccgccgaga tcgaccggcg
     5821 cgagaaggcc ggcgagcagc tcgagaccac gctggtgtcg ttcatcctcg ccgacatcgt
     5881 cgatgcgcgc atggacgggc gcaaggcgcg cgtgacggtc aagttcatga ccgagcaggt
     5941 caacgtgctg cgcgacgcca agggcgaggt ggtcgagggc gacccggcga cggtggccaa
     6001 gatcaccgac gtgtggacct tcgagcgcga cacccgcgcg cgcgacccga actggcagct
     6061 tgccgagacc cgcagcgacg actgaacgcg ggcggcgcg
//
LOCUS       DASWHU010000142         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_431573, whole genome shotgun sequence.
ACCESSION   DASWHU010000142 DASWHU010000000
VERSION     DASWHU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431573"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VGB88_05350"
     CDS             <1..163
                     /locus_tag="VGB88_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9646901.1"
                     /translation="HCESVQDYVSRDLLRRILAGEEEHVDWLETQFGLIERIGIETYI
                     QLQSAPAED"
     gene            complement(582..785)
                     /locus_tag="VGB88_05355"
     CDS             complement(582..785)
                     /locus_tag="VGB88_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9646902.1"
                     /translation="MYVCLCNGFRDSEIRAAARAGSGSPHAAYAALGGGVCCGRCVEL
                     AQDVIDEARAANQAAASVAVSAG"
     gene            complement(882..2741)
                     /locus_tag="VGB88_05360"
     CDS             complement(882..2741)
                     /locus_tag="VGB88_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646903.1"
                     /translation="MIDRGDKRRRRLAGAALLLVLCGLAAGARADGAAPVPLMSLDDR
                     GADLPPVGRSLFDFLVAKQVDGAWVLDVPYPFEALIEKLNAGLRSGYREPVKRVLHPL
                     GRSLHRHAAAPDYFRFPRATAAVDTEPAPQAGESGPMLKDRLYLGYQPLTDSIELIAY
                     NEAAGRFEYQVVTDYRDGGTPRVTYADRGVCTACHHNQALIYATQPWAESNANQRIAA
                     LLRAQADSFYGIPAQVPFDIPESFDEATDRANLFAAYQRAWRDGCGAAGERADAVACR
                     RGGLIAALRYRLTGGYQLGEAGDGARRAFAQGVAKGWRALWPGGVEIPNPNLADFDPL
                     LESGYGTGRFDAAEGLSRLVALADTDLLQFDERTEPLYERPPLETWRVAAPLAGVGLV
                     EPAWINQVIAGLGDFLAAADIERLDARLVAAAGPGRTESFACEVTPGAAEAGALAVLF
                     RCDDTAALGLLLKGRLRANADGAAEGVIDRVQSTSAAPGGLALARATVARDGESWHAR
                     FALTDSITGLNARLADGALLRRVELSWPAEAADGTVAAAATLTVADDFALLAAAVDRL
                     AAATLAGDSDALDDAPFRRVSVLRALFADLGIAPLDWCCLDADHLPPPRLLME"
     gene            complement(2853..3404)
                     /locus_tag="VGB88_05365"
     CDS             complement(2853..3404)
                     /locus_tag="VGB88_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9646904.1"
                     /translation="MPALIGDAGDVLIVAGLAGTAKDVAALTGDGAGAFALAGAMGGA
                     AMIGLGLALAQPRRRVLVVTGDGELLMNLGALATIAVINAPNLAILCVDNGHYGETGW
                     QESHTSRGVDLERIAAGAGIAAARTVGTADQIPAAAALLRRGNGTRFVVVRVAPTEPP
                     AAKRLMDAALCRSRFRQALLGRP"
     gene            complement(3466..4002)
                     /locus_tag="VGB88_05370"
     CDS             complement(3466..4002)
                     /locus_tag="VGB88_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX9646905.1"
                     /translation="MAPARARNPARPAWPDRLYAELRAGGVSQFAYVPDAGHAVLIAR
                     ALADRDVQAIALTTEEEGVALLAGADLGGARGVLLMQSSGVGNCVNMLSMIKSGRFPF
                     LTLVSMRGDFGEGNPWQVPMGQATETVLGAMGLVCLRVDAPGAVVPTARAALTMAFGA
                     GNAVAVLLTQKLIGAKKF"
     gene            complement(4014..4727)
                     /locus_tag="VGB88_05375"
     CDS             complement(4014..4727)
                     /locus_tag="VGB88_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX9646906.1"
                     /translation="MPGSRRAIRGPALRLSRRRMIGGLAAGVAVSAAGLVPARAFAAA
                     CGATPAQMTGPFYPLVAGEQDADLTRIAAGGSRALGEVIEVTGRVLGTNCEPLADAVI
                     EIWQANAAGRYDHPRDPSPRPLDPNFQGYARLAVDGEGRYRFTTIKPGAYPVDFGPVE
                     NWTRPPHIHFKVHQTFAPTATTQMYFAGEALNDTDLLLNAVPAGQRSALIVDFADTRA
                     DGVARGSFDIVLAGTMPSG"
     gene            4911..>5326
                     /locus_tag="VGB88_05380"
     CDS             4911..>5326
                     /locus_tag="VGB88_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX9646907.1"
                     /translation="MTYRPRDWGSHPPLIHPPYKSTVKRGPTKPLVPLAQSLSELTGP
                     VFGHDSLGELDNDLTRNARVNGEPIGERIVVTGRVMDEDARPLSDTLVEVWQTNAAGR
                     YVHRADQHHAPLDPNFLGAGRCITDDDGVYKFTSIMP"
BASE COUNT          750 a         1949 c         1966 g          661 t
ORIGIN      
        1 ccactgcgag agcgtccagg actacgtgtc gcgcgacctg ctgcgccgga tcctcgccgg
       61 cgaggaggag catgtcgact ggctcgagac ccagttcggc ctgatcgagc gcatcggcat
      121 cgagacctac atccagctcc aaagcgcgcc cgccgaagac tgatagcgtg tgaagcaatc
      181 gccggacgat cccttcctcg tcattcccgc gcaagagact gtgaaggaat caccggacgg
      241 ccccttcatc gtcatgcccg cgaaagcgga catccagact tttcaatgag ttactggacc
      301 tccgcttgcg cgggggtgac gccattgggc catggccggg gattccttca cgggctctga
      361 gggggcgccg ggcaggcgcg ctcagcgggc ggtcgaggcc tgcgccttgg ccggggccgc
      421 cgtcggctcc gagaccgctg acggcgggcc gtcgccgccg gacgcggatg gacgctcctc
      481 ggccgggccg ggggcgcgga cggcgccccg gaccgcgcgc gccagcgagg cgatccgccg
      541 ggggtcgagg ccacgccagg cgtacatcgc cgcccccgcc ctcagcccgc ggacacggcg
      601 acagaggccg ccgcctgatt tgcggcgcgc gcctcgtcga tcacgtcctg ggcgagctcg
      661 acgcagcgcc cgcagcacac cccgccgccg agcgcggcgt aggccgcatg cggcgagccg
      721 ctgccggcgc gcgccgccgc gcggatctcc gaatcgcgaa agccgttgca cagacagacg
      781 tacacgcgcg cctccttgcc cggccgtgag tatggtgcga gtgagaacga ttgtcaaccg
      841 catatccgtc gcgggcgcgc ggccgggcgc tatgggcggc gtcactccat cagcaggcgc
      901 ggcggcggca ggtggtcggc gtcgaggcag caccagtcga gcggggcgat gccgaggtcg
      961 gcgaacagcg cccgcagcac ggaaacccgc cggaacgggg cgtcgtcgag ggcgtcgctg
     1021 tcgccggcca gggtcgccgc ggccaggcgg tcgaccgcgg cggcgagcaa ggcgaaatcg
     1081 tcggcgacgg tgagcgtcgc ggccgccgcg accgtcccgt cggcggcctc ggccggccag
     1141 gacagctcca ccctgcgcaa cagggcgccg tcggcgaggc gggcattgag cccggtgatt
     1201 gagtcggtga gggcaaagcg cgcgtgccag gactcgccgt cacgcgcgac cgtggcccgc
     1261 gcgagcgcca gcccgccggg agccgccgag gtcgactgca cgcggtcgat gacgccctcc
     1321 gccgcgccat ccgcgttggc gcgcagccgg cccttgagca acaggccgag cgcggcagtg
     1381 tcgtcgcagc ggaacagcac ggccaaagcg ccggcctcgg ccgcgcccgg cgtgacctcg
     1441 caggcgaagc tctccgtgcg gcccggtccc gccgccgcga cgaggcgggc gtcgagccgc
     1501 tcgatgtcgg cggcggcgag gaagtcgccg agcccggcga tcacctgatt gatccaggcc
     1561 ggctcgacca gccccactcc ggcgagcggc gccgccaccc gccaggtctc gagcgggggg
     1621 cgctcgtaca agggctcggt gcgctcgtcg aactgcaaca ggtcggtgtc ggcaagcgcg
     1681 accagccggc tgaggccctc ggcggcatcg aacctgccgg tgccgtaacc cgactcgagc
     1741 agtgggtcga agtcggcgag gttcgggttc ggaatctcga cgccgcccgg ccacagcgcg
     1801 cgccagccct tggccacccc ttgggcgaag gcccggcgcg cgccgtcgcc cgcctcgccg
     1861 agctggtagc cgccggtcag ccggtagcgc agcgccgcaa tcaggccgcc gcgccggcag
     1921 gcgacagcgt cggcgcgctc gcccgccgcc ccgcagccgt cgcgccaggc gcgctggtag
     1981 gcggcgaaca ggttggcgcg gtcggtcgcc tcgtcgaagg attcggggat gtcgaacggc
     2041 acctgcgccg ggatgccgta gaagctgtcg gcctgcgcgc gcagcagcgc ggcgatgcgc
     2101 tggttggcgt tgctctcggc ccagggctgg gtggcgtaga tcagcgcctg attgtggtgg
     2161 caggcggtgc acacgccgcg gtcggcgtag gtgacgcggg gcgtgccgcc gtcgcggtag
     2221 tcggtgacca cctggtactc gaagcgcccc gcggcctcgt tgtaggcgat cagctcgatc
     2281 gagtcggtca gcggctgata gccgaggtag agccggtcct tgagcatcgg cccggactcg
     2341 ccggcctgcg gcgccggctc ggtgtcgacc gcggcggtcg cgcgcgggaa gcggaagtag
     2401 tccggcgccg cggcgtggcg gtggagcgag cggccgagcg gatggagcac gcgcttgacc
     2461 ggctcgcgat agccggacct gagaccggcg ttgagcttct cgatcagcgc ctcgaacgga
     2521 tagggcacgt ccagcaccca ggcgccgtcg acctgtttcg ccaccaggaa gtcgaacagc
     2581 gagcgcccga ccggcggcag gtcggcgccg cggtcgtcga gcgacatcag cggcaccggc
     2641 gccgccccgt ccgcccgcgc cccggccgcg aggccgcaca ggacgaggag cagggccgcc
     2701 ccggccagcc gccgccggcg cttgtcaccg cgatcgatca cgccgcctct cccaagtccg
     2761 cgcgccaaaa aagtccgcca ccatagccgc aggcgcaggg ctctacctag acctcgatca
     2821 gccctcggcc atgcgcctgg cgcgcggcag ggtcacggcc ggccgaggag ggcctggcgg
     2881 aagcggctgc ggcagagggc ggcgtccatc agccgcttgg ccgccggcgg ctcggtcggg
     2941 gccacccgga cgaccacgaa gcgggtgccg ttgccgcgcc gcagcagcgc tgccgcggcc
     3001 gggatctggt ccgcggtccc gaccgtgcgc gcggccgcga tgccggcacc ggcggcgatc
     3061 cgctccaggt cgacgccacg gctggtgtgg ctctcctgcc agccggtctc gccgtaatgg
     3121 ccgttgtcga cgcacaggat ggcgaggttg ggcgcattga tcacggcgat ggtggcgagc
     3181 gcgccgaggt tcatcagcag ctcgccgtcg ccggtgacca ccagcacccg ccgccggggc
     3241 tgggcgagcg cgagcccgag gccgatcatc gccgccccgc ccatggcgcc ggcaagcgcg
     3301 aaggcgcccg cgccgtcgcc ggtcaaggcg gccacgtcct tggcggtgcc ggcgagcccg
     3361 gcgacgatca ggacgtcgcc ggcatcgccg atcagcgccg gcacggcgtc gcgccgatcg
     3421 agggtgggac gagcaggtgc gggtgcgcgg cgcctcgcca tcggctcaga acttcttggc
     3481 cccgatcagc ttctgggtca gcagcacggc gaccgcgttg ccggcgccga acgccatggt
     3541 cagcgcggcg cgcgcggtgg gcacgacagc gcccggcgca tcgacgcgca ggcacaccag
     3601 ccccatcgcg ccgagcaccg tctcggtcgc ctggcccatc gggacttgcc acgggttgcc
     3661 ctcgccgaag tcgccgcgca tgctgaccag ggtcaggaac gggaaccgcc ccgacttgat
     3721 catcgacagc atgttgacgc agttgccgac gccgctcgac tgcatcagca gcaccccgcg
     3781 cgcaccgccc aagtcggcgc cggcgagcag cgccacccct tcctcctcgg tggtcagcgc
     3841 gatcgcctgc acgtcccggt cggcgagcgc gcgcgcgatc agcaccgcgt ggccggcgtc
     3901 gggcacgtag gcgaactggc tgacgccgcc cgcgcgcagc tcggcgtaca gccggtccgg
     3961 ccaggccggc cgggcggggt tgcgcgcgcg tgcgggcgcc acgccggcga ccgtcagccg
     4021 gacggcatgg tgccggcaag cacgatgtcg aagctgccgc gcgcgacgcc gtcggcgcgc
     4081 gtgtcggcaa aatcgacgat cagcgccgaa cgctgcccgg ccggcaccgc gttgagcaac
     4141 aggtcggtgt cgttgagcgc ctcgcccgcg aagtacatct gggtggtcgc ggtcggcgcg
     4201 aaggtctggt ggaccttgaa gtggatgtgc ggcggccggg tccagttctc gaccgggccg
     4261 aaatcgaccg gataggcgcc gggcttgatg gtggtgaacc ggtagcgccc ctcgccgtcg
     4321 acggcgaggc gggcatagcc ctggaagttg gggtcgagcg ggcgcgggct cgggtcgcgc
     4381 ggatggtcgt agcgcccggc ggcgttggcc tgccagatct cgatcaccgc gtcggcaagt
     4441 ggctcgcagt tggtgccgag cacgcgcccg gtgacctcga tcacctcgcc gagcgcgcgg
     4501 ctgccgccgg ccgcgatccg ggtcaggtcg gcatcctgct cgccggcgac gagcggatag
     4561 aacgggccgg tcatctgcgc cggcgtcgcc ccgcaggcgg cggcgaaggc gcgcgcggga
     4621 accagtcccg ccgcactcac cgcaacgccg gcggcgaggc cgccgatcat gcgccgccgc
     4681 gacagccgca gcgcgggtcc cctgatcgct ctcctgctgc ccggcatggc gtcctcccct
     4741 tttgtcttga caagcggctc tgcgtccaga cccggcgcga actgttccag aaagcgttcg
     4801 ggcccgtcaa tcgccttgcc gcgcgcgcac gccccctttc cgcgcttgac gcggacggcc
     4861 gggcaggctg gcatgtgcag cgcgcgcagc gatcggggag ggcgacacgc atgacctatc
     4921 ggccacggga ctggggctcg cacccgccgc tgatccaccc accctacaag tcgacggtca
     4981 agcgcggacc gaccaagccg ctggtgccgc tcgcccagtc gctgtcggag ctcaccgggc
     5041 cggtgttcgg ccacgacagc ctgggcgagc tcgacaacga cctgacccgc aacgcgcgcg
     5101 tcaacggcga gccgatcggc gagcgcatcg tggttaccgg gcgggtgatg gacgaggacg
     5161 cgcgcccgct cagcgacacc ctggtcgagg tctggcagac caacgccgcg gggcgctacg
     5221 tccaccgtgc cgaccagcac cacgcgccgc tcgatcccaa cttcctgggc gccgggcgct
     5281 gcatcaccga cgacgacggc gtctacaagt tcacctcgat catgcc
//
LOCUS       DASWHU010000143         5919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3028968, whole genome shotgun sequence.
ACCESSION   DASWHU010000143 DASWHU010000000
VERSION     DASWHU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3028968"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VGB88_05385"
     CDS             complement(<1..869)
                     /locus_tag="VGB88_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9646908.1"
                     /translation="MADDAFRFADELGPISIFHIHRPAAGLRAIVVVDNVACGPSIGG
                     ARMAPDVSLEECVRLARAMTFKSAAAGLPHGGGKSVIFADPRMAPAQKERLMRAFAHA
                     IADVRDYIVGPDMGTDERCMAWAKDEIGRAVGLPRELGGIPLDEIGATGFGLVAAIEV
                     AVEHLAMPLTGARVVIQGFGSVGRHAARFLAQQGAVLVATSDTQGTLADETGLDVAAL
                     IALKAEGRALRDHPHGRKLGGDAIVDIPCDIWIPAARPDVIHAGNVARLRTRVMAQGA
                     NIPCSPEAEAALA"
     gene            complement(902..1882)
                     /locus_tag="VGB88_05390"
     CDS             complement(902..1882)
                     /locus_tag="VGB88_05390"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9646909.1"
                     /translation="MPELTLVEAVRAALGRAMGEDERVVLFGEDIGIDGGVFRATDGL
                     YQRFGSARVRDTPLSEVLIAGLAVGLAAQGMRPVAEVQFMGFLYPTIDQLANHASRLR
                     TRTRGRLSCPMVLRAPFGGGIHAPEHHSESAESMLAHIPGLRVVIPSSPKRAYGLLLA
                     AIRDPDPLVFLEPKRLYRAAREQVDDDGAALPLDRCFVLREGGDVTLVSWGAMITETL
                     AAAEALAKANVAAEVIDVATLKPPDMATILASVGKTGRCAIIHEAPLTGGYGGEIAAR
                     LAEQAILELVAPIRRVAGYDTVMPLSRLERHYMPSPARIAAAARDLLAYQ"
     gene            complement(1875..2957)
                     /gene="pdhA"
                     /locus_tag="VGB88_05395"
     CDS             complement(1875..2957)
                     /gene="pdhA"
                     /locus_tag="VGB88_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX9646910.1"
                     /translation="MAETVARFEVARTAYIDADGRALGELPAFARDAEAMAALYRAMV
                     LTRAFDDKAIALQRTGRLGTYASSLGQEAVAVGTAAAMAPGDVLLPSFREHGAQLWRG
                     VSLFELLLYWGGDERGSDFKGPREDFPACIPVASHFPHGAGVALAFKMRGEARAAVCI
                     AGDGATSKGDFYEALNLVGVWRLPAVFVINNNQWAISMPRAEQTAAETLAQKAIAAGM
                     PGEQVDGNDVVAVQDAVARALVRARAGDGGTLIEALSYRLGDHTTADDASRYREDSEV
                     APHRKLEPLLRLRAFMTDAGQWDDARDRALREACAAEVDAAAADYLASVPEPPQAMFD
                     HLFEHLPEDVQAQRAAVIAAHGKGDA"
     gene            complement(2950..4653)
                     /locus_tag="VGB88_05400"
     CDS             complement(2950..4653)
                     /locus_tag="VGB88_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9646911.1"
                     /translation="MASERLSYVHGACETALLGLTVGQAFDRAVARWPERESLVVRHQ
                     DIRWTWVELARRVDEFAAGLVALGLEPGSRIGIWSPNNAEWVVTQFASAKAGLVLVNV
                     NPAYRLPELDYALNTVEVGALVTAPSLKTSDYIAMLGELCPELATAAPGRLSAQRVPS
                     LRHVIRLGPEASAGMHSFDDIAAGATDADRRRIAALADLLQFDDAINIQFTSGTTGRP
                     KAATLTHHNIVNNAYFTGLRMRLSERDRLCIPVPMYHCFGMVLGTLCCVTHGATMVFS
                     SAGFDAGAVLETIEAERCTALHGVPTMFIAALDHPDFARRDLSSLRTGIMAGAPCPVE
                     LMKRVMDEMHLAEITIAYGMTETGPVSFQTAVDDPLERRVETVGRVLPHLEVKIVDDH
                     GRIVPRGVAGELMTRGYSVMPKYWNDPERTAEAIDQAHWIASGDIAVIDDAGYCRIVG
                     RLKDMLIRGGENVFPREIEEFLYRHPKVEEVEVFGVPDPKYGEEVCAWIKLRAGESAS
                     EAEIRGFCEGKLAHFKIPRYIRFVDAFPMTVTGKVQKFVMREEMARALEREGTERAAR
                     G"
     gene            4792..5790
                     /locus_tag="VGB88_05405"
     CDS             4792..5790
                     /locus_tag="VGB88_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX9646912.1"
                     /translation="MGTEDAMRICVIGAGAMGSIYGGLLAHSGEDVTLIDTWPEHVAA
                     IARGGLRLEGIGGALTVKVAAATEAAGDRLFDLALIQTDTNNTAAAAATAGRVLTPDG
                     FAVTLQNGIGNLEILCAALGAARVAGGLTYHSGAMRGPGHVAHTHAGPTWLGELDGRG
                     SARIADLAARLAKAGFTPHVVDNIVGTIWTKFIHNCAVNPVCAASGLRVGEIPRTPAA
                     DALQTRIIAEALEVVAAKGVALADADPMATIKAFCRKKFNKPSMLQHMEAGRRTEIDA
                     LNGALVREGRALGLATPYNQAVVWIIEAMQSQRMQAKSGPPVDYDALEREAQRQAS"
BASE COUNT          868 a         2145 c         2073 g          833 t
ORIGIN      
        1 tcggccaacg ccgcctcggc ctcggggctg cacgggatgt tggcgccctg cgccatcacc
       61 ctagtgcgca gccgcgcaac attgccggcg tggatcacgt cgggccgcgc cgccggtatc
      121 cagatgtcgc aaggaatatc gacaatcgca tcgccgccga gtttgcgccc gtgcgggtga
      181 tcgcgcagcg cccgcccctc ggctttcagc gcgatcagcg cagcaacatc gagcccggtc
      241 tcgtcggcga gggtgccctg cgtgtcgctt gttgccacca gcaccgcgcc ttgttgcgcc
      301 aggaatcggg cggcgtgcct gcccaccgac ccgaagccct ggatgaccac gcgagcgccg
      361 gtgagcggca tagcgagatg ttccacagcc acttctatcg cggcgacaag cccgaagccg
      421 gtggcgccga tctcgtcgag cggtatgccg ccgagctcgc gcggcaggcc gaccgcgcgc
      481 ccgatctcgt ccttcgccca cgccatgcag cgctcgtcgg tgcccatgtc ggggccgacg
      541 atgtagtcgc ggacgtcggc gatggcatgc gcgaaggcgc gcatcaggcg ctccttctgc
      601 gccggcgcca tgcgcgggtc ggcgaagatc accgacttgc cgccgccgtg gggaagcccg
      661 gcggccgcgc tcttgaaagt catcgcgcgc gccagccgca cgcattcctc gaggctcacg
      721 tcgggcgcca tgcgcgcgcc gccgatcgac ggcccgcagg cgacgttgtc gaccaccacg
      781 atcgcgcgca gccccgccgc cggccggtgg atgtgaaaga tgctgatcgg gccgagctcg
      841 tcggcgaagc ggaatgcgtc gtcagccatg cccgatcagc ctcctcgtgc tgcggcgcgg
      901 atcactgata ggcaagcagg tcgcgcgccg cggccgcgat gcgtgcgggc gaaggcatgt
      961 agtggcgctc gagccgcgac agcggcatca cggtatcgta gccggcgacg cggcggatcg
     1021 gcgccaccag ctcgaggatg gcctgctcgg ccaggcgcgc cgcgatctcg ccgccatagc
     1081 cgccggtgag cggtgcctcg tggatgatcg cgcagcggcc ggtctttcct accgaggcga
     1141 ggatcgtcgc catgtcgggc ggcttgagcg tggcgacgtc gatcacctcg gcggcgacat
     1201 tcgccttcgc cagcgcctcg gctgccgcga gggtctcggt gatcatcgcg ccccagctga
     1261 ccagggtcac gtcgccgccc tcgcgcagca cgaagcagcg gtcgagcggc agcgcagcgc
     1321 cgtcgtcgtc gacctgctcg cgcgcggcgc ggtagagccg cttgggctcg aggaacacca
     1381 gcgggtcggg atcgcggatc gccgctaaca gcaagccgta agcgcgcttc ggcgaggacg
     1441 ggatcaccac ccgcaggccg gggatgtggg cgagcatcga ctcggcactc tccgaatggt
     1501 gctcgggcgc gtggatgccg ccgccgaacg gcgccctcag caccatcggg cagctcagcc
     1561 gcccgcgggt gcgcgtgcgc agccgcgagg catggttggc gagctgatcg atggtcgggt
     1621 agaggaagcc catgaactgg acctcggcca ccgggcgcat gccctgggcg gcaaggccca
     1681 ccgccagccc ggcgatcagc acctcggaca gcggcgtgtc gcgcaccctg gccgagccga
     1741 accgctgata gaggccgtcg gtggcacgga acacgccgcc gtcgataccg atgtcctcgc
     1801 cgaacaggac gacgcgctcg tcctcgccca tcgcgcggcc gagcgcggcg cgcaccgcct
     1861 cgaccagcgt cagctcaggc atcgcccttg ccgtgcgccg cgatcacggc ggcgcgctgg
     1921 gcttgcacgt cctcgggcag gtgctcgaac agatggtcga acatcgcctg cggcggctcg
     1981 ggcacgctcg ccagatagtc cgcggcggcg gcgtcgacct cggccgcgca cgcctcgcgc
     2041 agcgcccggt cgcgcgcatc gtcccactgg ccggcgtcgg tcatgaacgc gcgcaggcgc
     2101 agcagcggct cgagcttgcg gtgcggcgcg acctccgagt cctcgcggta gcggctggcg
     2161 tcgtcggcgg tggtgtggtc gccaagccgg tagctgagcg cctcgatcag ggtgccgccg
     2221 tcgccggcgc gcgcgcgcac gagcgcccgc gccaccgcgt cctgcaccgc gaccacgtcg
     2281 ttgccgtcga cctgctcgcc cggcatgccg gccgcgatcg ccttctgggc gagggtctcg
     2341 gccgcggtct gctcggcgcg cggcatcgag atcgcccact gattgttgtt gatcacgaac
     2401 accgccggca gccgccatac gcccaccagg ttgagcgcct cgtagaagtc gcccttgctg
     2461 gtcgcgccgt cgccggcgat gcacaccgcg gcgcgcgcct cgccccgcat cttgaacgcc
     2521 agcgcgaccc cggcgccgtg ggggaagtgg ctggccaccg ggatgcacgc cgggaagtcc
     2581 tcgcgcgggc ccttgaagtc gctgccgcgc tcgtcgccgc cccagtagag caacagctcg
     2641 aagaggctca ccccgcgcca cagctgcgcg ccgtgctcgc gaaacgacgg cagcagcacg
     2701 tcgcccggcg ccattgccgc cgccgttccg accgcgaccg cctcctggcc gagcgaggac
     2761 gcataggtgc cgagccggcc ggtgcgctgg agcgcgatcg ccttgtcgtc gaaggcgcgg
     2821 gtcagcacca tggcgcggta gagcgccgcc atcgcctcgg cgtcgcgggc gaaggcgggc
     2881 agctcgccca gcgcccggcc gtcggcatcg atataggccg tgcgcgcgac ctcgaaccgc
     2941 gcgacggtct cagccacgcg ccgcgcgctc cgtgccctcg cgctcgagcg cgcgcgccat
     3001 ctcctcgcgc atcacgaact tctggacctt gccggtcacg gtcatcggga aggcatcgac
     3061 gaagcggatg tagcgcggaa tcttgaagtg cgcgagcttg ccctcgcaga agccgcggat
     3121 ctcggcctcg ctcgcgctct cgcccgcgcg cagcttgatc caggcgcata cctcctcacc
     3181 gtatttggga tcgggaacgc cgaacacctc gacctcctcg accttcgggt gccggtatag
     3241 gaactcctcg atctcgcgcg ggaagacgtt ctcgccgccc cggatcagca tgtccttgag
     3301 gcggccgacg atgcggcagt aaccggcgtc gtcgatcacc gcgatgtcgc cgctcgcgat
     3361 ccagtgcgcc tggtcgatcg cctccgccgt gcgctccggg tcgttccagt atttgggcat
     3421 caccgagtag ccgcgggtca tcagctcgcc cgcgaccccg cgcggcacga tccggccgtg
     3481 gtcgtcgaca atcttgacct cgaggtgcgg cagcacccgc cccacggtct cgacccgccg
     3541 ctcgagcgga tcgtcgaccg cggtctggaa gctcaccggc ccggtctcgg tcatgccgta
     3601 ggcgatggtg atctcggcca agtgcatctc gtccatcacc cgcttcatca gctccaccgg
     3661 gcacggcgcg ccggccatga tgccggtgcg caggctggag aggtcgcggc gggcgaagtc
     3721 ggggtggtcg agcgcggcga tgaacatggt cggcaccccg tgcagcgccg tgcagcgctc
     3781 ggcctcgatc gtctccagca ccgcgccggc gtcgaacccg gccgacgaga ataccatcgt
     3841 tgccccgtgg gtgacgcagc acaaggtgcc gagcaccatg ccgaagcaat ggtacatggg
     3901 caccgggatg cacagccggt cgcgctcgct gagccgcatc cgcaggccgg tgaaataggc
     3961 gttgttgacg atgttgtggt gggtcagcgt cgccgccttc ggccggccgg tggtgccgct
     4021 ggtgaactgg atgttgatcg catcgtcgaa ctggagcagg tcggcgagcg cagcgatgcg
     4081 ccgccggtcc gcgtccgtcg cgccggccgc aatgtcgtcg aagctgtgca tgccggcgct
     4141 cgcctcgggc ccgagccgga tgacgtggcg gaggctcggc acgcgctgcg cgctgagccg
     4201 ccccggcgcg gcggtggcaa gctcggggca cagctcgccg agcatcgcga tgtagtcgct
     4261 ggtcttcagg ctcggcgcgg tgaccagcgc gccgacctcg accgtattga gcgcgtagtc
     4321 gagctcgggc aggcggtagg cgggattgac gttgaccagc accaacccgg ccttggcgct
     4381 ggcgaactgg gtgaccaccc actcggcgtt gttgggcgac cagatgccga tgcgcgagcc
     4441 cggctcgagg ccgagcgcga ccagcccggc ggcgaactcg tcgacccgcc gggcgagttc
     4501 cacccacgtc caacggatgt cctggtggcg cacgaccagc gactcgcgct ccggccagcg
     4561 cgcgaccgca cggtcgaagg cctggcccac ggtcaagccg agcagcgccg tctcgcaggc
     4621 gccgtggacg tagctcagcc gttcgctcgc catcatcggt accggtcgat cgttttgcaa
     4681 attgtcccgc gcccgcctgg ccgggcctta aaatgcatca agcgccacgc cggcgcactt
     4741 gcgatttcgc cgccgccgtc ccagcatccg gcgggccgcg accggcggcc gatgggcacg
     4801 gaggacgcca tgcgcatctg cgtgatcggc gccggggcca tgggatcgat ctatggcggg
     4861 cttctggcgc acagcggcga ggacgtgacg ctgatcgaca cctggccgga gcatgtcgcg
     4921 gcgatcgcgc gcggcggcct caggctcgag ggcatcggcg gcgcgcttac cgtcaaggtc
     4981 gcggcggcga ccgaggccgc gggcgaccgc ctgttcgacc tcgcgctgat ccagaccgac
     5041 accaacaaca ccgccgccgc cgccgcgacc gcagggcgcg tgcttacgcc ggacggcttc
     5101 gcggtcaccc tgcagaacgg catcggcaac ctcgagatcc tgtgcgcggc gctcggcgcg
     5161 gcgcgggtcg cgggcgggct gacctatcac agcggcgcaa tgcgcggccc cggccatgtc
     5221 gcccacaccc atgccgggcc gacctggctc ggcgagctcg acggccgggg gagcgcgcgc
     5281 atcgcggatc tggccgcgcg gctcgccaag gccggcttca cgccccacgt ggtcgacaac
     5341 atcgtcggca ccatctggac caagttcatc cacaactgcg cggtcaaccc ggtatgcgcg
     5401 gcgagcggcc tgcgcgtcgg cgagatcccg cgcacgccgg cggcggacgc gctccagacc
     5461 aggatcatcg cggaagcgct tgaggtggtc gcggccaagg gcgtggcact cgccgacgcc
     5521 gacccgatgg cgacgatcaa ggcgttctgc cggaagaagt tcaacaagcc gtcgatgctc
     5581 cagcacatgg aggccggccg gcgcaccgag atcgacgcgc tcaacggcgc gctggtgcgc
     5641 gagggccgcg cgctcgggct ggcgacgccc tacaaccagg ccgtggtgtg gatcatcgag
     5701 gctatgcaga gccagcgaat gcaggccaag agcggcccgc cggtcgacta cgacgcgctc
     5761 gaacgcgagg cgcagcggca ggcaagctag gccgaaaccc aagccgggaa acgaatcggc
     5821 acacgaatcg gacccgcttg agtcgcccaa actccctttc cgggccgcgc ggcaagcgat
     5881 tttcaggtgc cttcgttcgg ccgtggactc aatgccgcc
//
LOCUS       DASWHU010000144         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2291948, whole genome shotgun sequence.
ACCESSION   DASWHU010000144 DASWHU010000000
VERSION     DASWHU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291948"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VGB88_05410"
     CDS             complement(<1..1137)
                     /locus_tag="VGB88_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9646913.1"
                     /translation="MTGAAAAARDPLARGLAEHRAGRLDPAEGWYRDALTVDPDNARA
                     LHLLGLLRLQQGDAQAAMPLLRRAAGRDPADADCRNRLGAAYAALGNRIEAARAYRGA
                     LALDPDHAEAHSNLGMLLAESGDVDAAVEEYQRAIAAAPDFAEAHNNLAVALAGIGRV
                     EDAERHFRTALGLRPGYADARANLGDLMLRLGDLARAKAELRAAIDIAPDHLQARLLM
                     ADALMRQGALEGALDEAQIALSVAPESARAHNCYGMICQQTGGLHRGLAHLDHALALD
                     PGLADAHGNRAIALTALGRLDEAEGAARRAVELAPEFAVHHLNLALILLLRGKLEEGF
                     AEYRWRFRTGLPWLEPRRFDQAAWHGEPLDGKAVLAWGEQGVGEE"
     gene            complement(1134..3497)
                     /locus_tag="VGB88_05415"
     CDS             complement(1134..3497)
                     /locus_tag="VGB88_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9646914.1"
                     /translation="MAGASVATRTRAGEAHRLVGAALALHRRGQLAAAEPLYRQAIET
                     DPDAAEPLHLLGMLALQTGRPCDAARLLERAAELDPDNAVIRNRLGVACAQAGRRDEA
                     RAHYRAALARAPRLAEAHNNLGVLLADDGALDEAMAHYRRAIEADPGSAEACNNLGVA
                     LARQGDGEAAERAYRQALAANPDYPDAHNNLGALWRERGDDAAAAEQFRTALALAPDF
                     AEAHNNLGICLTRLGDTERATACFARAVALAPHLVTSRFHLGNLLLARGDGAGAAAAF
                     AAVLEREPAHYDALVGLGRAELECDRDEVALGHFRTALGVRAEPVAALNGAALASRRL
                     GRPKEAEALHRRALALYPDGVDTLIDLANLLLAEDRPEEAMALYARVLALEPESTHAH
                     NNLGLALGQLGRIDEAVAAFRRAIAIKPDYAQAHNNLGNVLNAAARFDDAFASYRTAL
                     DLDPGLASARLNLGMAMLLHGDFGAGFDAYEGRWRVPETPRRKFAPPHWQGEPLAGRT
                     ILVWGEQGAGDEIMLASVLPDLIERAGRCVIECDRRLAPVFARSFAEAEVVARAEPAD
                     ARTLDAAIEVQSPLGALCRWLRRDQDAFLRSRRAYLVADAARVDALRARTDALGAGLK
                     VGIAWRSKTPRWGRVKTASLELWEPLLTRPGVQFVNLQYGDVDDELDQIERRCGVRVH
                     RDPFVDQMASLDDFASQVSALDLVISISNTTVHMAGALGVPVWTLLSHVPDWRWQLGR
                     ADALWYPTMRLFRQRRLGDWQGVIAEVAERLSRVVAGKVPPAEGSAR"
BASE COUNT          473 a         1302 c         1353 g          454 t
ORIGIN      
        1 ctcctcgccc accccctgct cgccccacgc gagcaccgcc ttgccgtcga gcggctcgcc
       61 gtgccaggcg gcctggtcga agcggcgcgg ctcgagccac ggcaggccgg tgcggaagcg
      121 ccagcgatac tcggcgaaac cctcctcgag cttgccgcgc aacagcagga tcagcgccag
      181 gtttaggtgg tgcacggcga actcgggcgc gagctcgacc gcgcgcctgg ccgcgccctc
      241 ggcctcgtcg agccgcccga gcgcggtcag cgcgatcgcc cggttgccgt gggcgtcggc
      301 gagccccgga tccagcgcca acgcgtgatc tagatgggcg agaccgcgat gcaggccgcc
      361 ggtctgctgg cagatcatgc catagcaatt atgagcccgc gcggactcgg gcgccaccga
      421 cagggcgatc tgggcctcgt ccagcgcgcc ctcgagcgcg ccctggcgca tcaacgcatc
      481 cgccatcaac agccgcgcct gcaagtggtc cggcgcgatg tcgatggcgg cgcgaagctc
      541 ggccttggcg cgcgcaagat cgccgagacg gagcatcagg tcgcccagat tggcgcgcgc
      601 gtcggcatat ccgggcctga ggcccagcgc ggtgcggaaa tgccgctcgg cgtcctcgac
      661 ccggccgatc ccggccagcg ccaccgccag gttgttgtgc gcctcggcga aatcgggcgc
      721 ggccgcgatc gcgcgctggt actcttcgac cgcggcgtcg acatcgccgg actcggccag
      781 cagcatgccg aggttgctgt gcgcctcggc gtggtcggga tcgagcgcga gggcgccgcg
      841 ataggcgcgc gccgcctcga tgcggttgcc gagcgcggcg taggcggcgc cgaggcggtt
      901 gcggcagtcg gcgtcggcgg gatcgcgccc ggccgcccgc cgcaacagcg gcatcgcggc
      961 ttgggcgtcg ccctgctgga ggcggagcag ccccagcagg tggagcgccc gcgcattgtc
     1021 gggatcaacg gtgagcgcgt cgcggtacca cccctccgcc gggtccagcc ggccggcgcg
     1081 gtgctcggcg agaccgcgcg ccagcggatc gcgcgcggcc gccgcggcgc cggtcatcgg
     1141 gcggagcctt ccgccggcgg gaccttgccg gcgaccacgc gcgaaagccg ctcggcgacc
     1201 tcggcgatga cgccctgcca gtcgccgagg cgccgctggc ggaacaggcg catggtcgga
     1261 taccaaagcg cgtcggcgcg gccgagctgc cagcgccagt cggggacgtg gctgagcaag
     1321 gtccacaccg gcacgccgag cgcgccggcc atgtgcacgg tggtattgga gatcgagatc
     1381 accaggtcga gggcggagac ctgcgaggcg aaatcgtcga ggctcgccat ctggtcgacg
     1441 aacgggtcac ggtggactcg cacgccacat cggcgctcga tctggtcgag ctcgtcatcg
     1501 acatcgccat attgcaggtt gacgaactgc acgcccggcc gggtcagcag cggttcccac
     1561 agctcgagcg aggcggtctt gacccgcccc cagcggggcg tcttgctgcg ccaggcgatg
     1621 ccgaccttga gccccgcacc gagcgcgtcg gtgcgtgcgc gcaaggcgtc cacgcgcgcc
     1681 gcgtcggcga ccagataggc gcgccggctg cgcaggaagg cgtcctggtc gcggcgcagc
     1741 cagcggcaga gcgcgccgag cgggctttgc acctcgatcg ccgcatcgag cgtgcgcgca
     1801 tccgccggct cggcgcgcgc caccacctcg gcctccgcga acgagcgggc gaacaccggc
     1861 gcgagccgcc ggtcgcactc gatcacgcag cgcccggcgc gctcgatcag gtcgggcagc
     1921 accgaggcga gcatgatctc gtcgcccgcg ccctgctcgc cccacaccag gatggtgcgg
     1981 cccgcaagcg gctcgccctg ccagtgcggc ggcgcgaact ttcggcgcgg cgtctccggc
     2041 acccgccagc gtccctcgta ggcgtcgaag ccggcgccga agtcgccgtg cagcagcatc
     2101 gccatgccga gattgaggcg cgcgctggcg agccccgggt cgaggtcgag cgcggtgcgg
     2161 tagctggcga aggcgtcgtc gaaccgggcc gcggcgttga gcacgttgcc gaggttgttg
     2221 tgcgcctgcg cgtagtcggg cttgatcgcg atggcgcggc ggaatgccgc caccgcctcg
     2281 tcgatccgcc cgagctggcc gagcgcgagg ccgagattgt tgtgcgcgtg ggtggactcg
     2341 ggctcgagcg caagcacgcg ggcgtagagc gccatcgcct cctcgggccg gtcctcggcg
     2401 agcagcaggt tggcgaggtc gatcagggtg tcgacgccat caggatagag cgcgagcgcg
     2461 cgccggtgca gcgcctcggc ctctttcggc cggccgaggc ggcgcgatgc gagtgccgcg
     2521 ccgttgagcg ccgcgaccgg ctccgcgcgc acgcctagcg cggtccggaa atgtccgagc
     2581 gcgacctcgt cccggtcgca ttcgagctcc gcgcggccga ggccgaccag ggcgtcgtag
     2641 tgtgccggct cgcgctcgag cacggccgcg aacgcggcgg cggcaccggc gccgtcgccg
     2701 cgcgcgagca aaaggttgcc aagatggaac cggctcgtaa ccaggtgcgg cgccagcgcc
     2761 accgcgcgcg cgaagcacgc ggtcgcgcgc tcggtgtcgc caagccgggt caggcagatg
     2821 ccgagattgt tgtgcgcctc ggcgaagtcg ggcgccagcg cgagcgcggt gcggaattgc
     2881 tcggcggccg cggcatcgtc gccgcgctcg cgccacaagg cgccgaggtt gttgtgcgcg
     2941 tccggataat ccgggttggc ggcgagcgcc tgacggtagg cacgctccgc cgcctcgccg
     3001 tcgccctggc gggcgagcgc gacgccgaga ttgttgcacg cctcggccga gcccgggtcg
     3061 gcctcgatcg cacgccgata atgcgccatc gcctcgtcca gcgcgccgtc gtcggcgagc
     3121 aggacgccga gattgttgtg cgcctcggca agccgcgggg cgcgcgccag tgccgcacgg
     3181 taatgcgcgc gcgcctcgtc gcgccggccg gcctgggcgc aggcgacgcc gagccggttg
     3241 cggatcaccg cgttgtcggg atcgagctct gccgcgcgct cgagcaggcg cgctgcgtcg
     3301 caaggccggc cggtctgcag cgccagcatg ccgagcagat gcagcggctc cgcggcatcg
     3361 ggatcggtct cgatcgcctg gcggtaaagc ggctcggcgg cggcgagctg gccgcgccgg
     3421 tgcaacgcca atgccgcgcc gaccaggcgg tgcgcctcgc ccgcacgagt ccgggttgcg
     3481 acgctcgccc ccgccatcgt cgcggcgcgc ccgtcggcgc tccgccccgc ggccggaccg
     3541 ccggagcggt gcgcgggcaa agcgcggccg ggttgactgg ga
//
LOCUS       DASWHU010000145        32289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3029587, whole genome shotgun sequence.
ACCESSION   DASWHU010000145 DASWHU010000000
VERSION     DASWHU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 32289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32289
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029587"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /gene="tuf"
                     /locus_tag="VGB88_05420"
     CDS             <1..210
                     /gene="tuf"
                     /locus_tag="VGB88_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390500.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX9646915.1"
                     /translation="NYRPQFYFRTTDVTGSVKLPEGTEMVMPGDNIAMEIELIAPIAM
                     DQGLRFAIREGGRTVGAGVVAEIVE"
     gene            240..548
                     /gene="rpsJ"
                     /locus_tag="VGB88_05425"
     CDS             240..548
                     /gene="rpsJ"
                     /locus_tag="VGB88_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX9646916.1"
                     /translation="MESQNIRIRLKAYDHRVLDQSTLEIVNTAKRTGAAVRGPIPLPT
                     HIERFTVLRSPHIDKKSREQFEIRTHKRLLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            580..1329
                     /gene="rplC"
                     /locus_tag="VGB88_05430"
     CDS             580..1329
                     /gene="rplC"
                     /locus_tag="VGB88_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX9646917.1"
                     /translation="MRTGVLARKLGMSRVFLDDGRHLPVTVLGIEDCRVVAQKTAERD
                     GYTALQVGAGRAKPKNTSKAMRGHFAKARVEPKLRLAEFRVTPDCLVEVGAELGAGHF
                     VSGQFVDVAGTSIGKGFAGAMKRHGFRGLGASHGVSLSHRAHGSTGQCQDPGRVFKGK
                     RMAGQMGAKRVTEQNLYVVEADAGRGLIVVMGGVPGPVGGYLRVSDAKKMALPEGVPM
                     PAGVRGAAVAAEVTEGEALAGDAGGTPDEKD"
     gene            1335..1955
                     /gene="rplD"
                     /locus_tag="VGB88_05435"
     CDS             1335..1955
                     /gene="rplD"
                     /locus_tag="VGB88_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9646918.1"
                     /translation="MKCDVITLDNGKAGDIELADEVFAVPVRADILQRVVVWQLAKRR
                     AGTHKVKGRSEVRATGVKLYRQKGTGRARHGSRKVNLFRGGGRSFGPVVRSHAIELPK
                     KVRRLALKCALASKRADGKLVVLDSATLEAPRTAELARRIAALGWHSALVIDGAEVND
                     NFRRAARNLPGLDVLPSQGANVYDILRSDLLVLTRAAVAALEARLR"
     gene            1952..2245
                     /locus_tag="VGB88_05440"
     CDS             1952..2245
                     /locus_tag="VGB88_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9646919.1"
                     /translation="MNAERACDIILGPVITEKATALSEHNQFAFRVRKDATKGEIRAA
                     IEALFGVKVTGVNTINVKGKVKRFRGHLGRRSDVKKAIVTLAEGQSLDYTAGI"
     gene            2265..3107
                     /gene="rplB"
                     /locus_tag="VGB88_05445"
     CDS             2265..3107
                     /gene="rplB"
                     /locus_tag="VGB88_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019297986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX9646920.1"
                     /translation="MALKSYRPITPSQRQLVLVDRSDLYKGKPVKKLTEGLTGSGGRN
                     NLGRMTVRHRGGGHKRRYRLIDLKRRKLGVAAVVERLEYDPNRSAFIALIRYDDGELA
                     YILAPQRLRPGDKVVADSRADIKPGNAMPMKNMPVGTIIHNVEMKPGKGGQIARAAGN
                     YAQLIGKDAGYALLRLSSGEQRMVHAECMATVGAVSNPDNQNINFGKAGRMRWLGRRP
                     KVRGVAMNPVDHPHGGGEGRTSGGRHPVTPWGVPTKGKRTRNNKATDRLILRSRHRAK
                     KKKR"
     gene            3119..3397
                     /gene="rpsS"
                     /locus_tag="VGB88_05450"
     CDS             3119..3397
                     /gene="rpsS"
                     /locus_tag="VGB88_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX9646921.1"
                     /translation="MARSVWKGPFVDGYLLKKAEVVHGSGRKEVIKTWSRRSTILPQF
                     VGLTFGVHNGHKFIPVLVTENMVGHKLGEFAPTRTYHGHSGDKKARRA"
     gene            3402..3779
                     /gene="rplV"
                     /locus_tag="VGB88_05455"
     CDS             3402..3779
                     /gene="rplV"
                     /locus_tag="VGB88_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX9646922.1"
                     /translation="MGKRTPERRLAENEAMAMARSLRTSARKLNLVAESIRGLSAETA
                     LATLGFSRRRIARDVRKVLQSAIANAENNHNLDVDRLVVAEAFVGKAITIRRWRPRAR
                     GRVGRIVKPFSRLTVVVREREED"
     gene            3787..4467
                     /gene="rpsC"
                     /locus_tag="VGB88_05460"
     CDS             3787..4467
                     /gene="rpsC"
                     /locus_tag="VGB88_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9646923.1"
                     /translation="MGQKVNPIGLRLGVNRTWDSRWYADNDYAALLHEDLKIRNFLEQ
                     RLAQAGVARIVIERPAKKARITIHTARPGVVIGKKGADIEKLRTDLSKLTASEVAINI
                     VEIRKPEIEAQLVAENISQQLERRVAFRRAMKRAVQSAMRLGALGIRITCAGRLGGAE
                     IARTEWYREGRVPLHTLRADVDYGTATARTAYGTCGVKVWVFKGEIMEHDPTAQDRRR
                     AELQGARV"
     gene            4495..4914
                     /gene="rplP"
                     /locus_tag="VGB88_05465"
     CDS             4495..4914
                     /gene="rplP"
                     /locus_tag="VGB88_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX9646924.1"
                     /translation="MLSPKRTKYRKAHKGRVHGAAKGGTTLNFGSYGLKASEPARVSA
                     RQIEAARRAITRHIKRAGRMWIRIFPDVPVSKKPTEVRMGKGKGAPEFWMARVKPGRI
                     MFELDGVPAQLARAAFARGAAKLPMRSRFVARIGEEA"
     gene            4919..5131
                     /gene="rpmC"
                     /locus_tag="VGB88_05470"
     CDS             4919..5131
                     /gene="rpmC"
                     /locus_tag="VGB88_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX9646925.1"
                     /translation="MRARELIAKGRDELSVQLRDLKKEQFNLRFQRATGQLENTARFR
                     QVRRDIARLKTVLGQQAAKPARESES"
     gene            5135..5371
                     /gene="rpsQ"
                     /locus_tag="VGB88_05475"
     CDS             5135..5371
                     /gene="rpsQ"
                     /locus_tag="VGB88_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX9646926.1"
                     /translation="MPRRILRGTVISDGADKTVVVRVERRFRHPLYKKFIRKSKKYAV
                     HDPDNRHKVGDVVRIRECRPVSKSKHWEVLGEGA"
     gene            5405..5773
                     /gene="rplN"
                     /locus_tag="VGB88_05480"
     CDS             5405..5773
                     /gene="rplN"
                     /locus_tag="VGB88_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9646927.1"
                     /translation="MIQMQTSLDVADNSGARRVQCIKVLGGTGRKTASIGDVIVVSVK
                     EAIPRGRVKKGDIHRAVIVRTAKEIRRPDGSAIRFDRNAAVLITPQGEPIGTRIFGPV
                     TRELRAKRFMKIVSLAPEVL"
     gene            5773..6096
                     /gene="rplX"
                     /locus_tag="VGB88_05485"
     CDS             5773..6096
                     /gene="rplX"
                     /locus_tag="VGB88_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9646928.1"
                     /translation="MANKLKIRKGDTVVVLCGKEKGRRGEVLRVIPDLRRVVVRGLNM
                     VKRHTRPSAVTPGGILEKEAPLHASNLALVDPKDGKPTRVGYRVLEDGRKVRFAKRSG
                     EVIDR"
     gene            6108..6647
                     /gene="rplE"
                     /locus_tag="VGB88_05490"
     CDS             6108..6647
                     /gene="rplE"
                     /locus_tag="VGB88_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9646929.1"
                     /translation="MARLREHYQTTVKPALMAQFGYRNPMQVPRLDKIVVNMGVGEAA
                     RDNKVIQGAEADLAALSGQKPLITRARKSIANFKLREGMTVGCKVTLRRERMYEFLDR
                     LINIALPRVRDFHGVSPASFDGRGNYALGLKEQIVFPEIDYDAVDAVRGLDVVIVTTA
                     ATDNEARALLRGFNMPFVG"
     gene            6685..6990
                     /gene="rpsN"
                     /locus_tag="VGB88_05495"
     CDS             6685..6990
                     /gene="rpsN"
                     /locus_tag="VGB88_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX9646930.1"
                     /translation="MAKRSAIERNNKRKRMVARYAAKRAALKAMANDESLAMEDRFAA
                     RLKLAEQPRNGAPSRVRNRCELSGRPRGYYRKLKLSRIALRDLASAGQIPGMVKSSW"
     gene            7004..7402
                     /gene="rpsH"
                     /locus_tag="VGB88_05500"
     CDS             7004..7402
                     /gene="rpsH"
                     /locus_tag="VGB88_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9646931.1"
                     /translation="MTMSDPLGDMLTRIRNGQRVHKAIVRCPASRLRVNVLEVLRKEG
                     YIRGFRRLDEEAVQPLLEIELKYQEGTPVIQEISRVSRPGQRVYSSIRDLARVYNGLG
                     ISILSTPRGVMSDADARAANVGGEVLCKVF"
     gene            7412..7945
                     /gene="rplF"
                     /locus_tag="VGB88_05505"
     CDS             7412..7945
                     /gene="rplF"
                     /locus_tag="VGB88_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9646932.1"
                     /translation="MSRIGKHPVSVPSGVEVAIAGQTLTVKGKRGALSRTLANEVEVR
                     RDGDQIWIKPRAEDRRSRAMWGLSRSLVNNMVLGVSAGFTRMLEISGVGFRAAVDGRY
                     LNLQLGYSHEVKLAVPPDVEITCPRPTAITITGADRQRVGQIAAEIRALRKPEPYKGK
                     GIRYAEEHVRRKEGKKK"
     gene            7958..8320
                     /gene="rplR"
                     /locus_tag="VGB88_05510"
     CDS             7958..8320
                     /gene="rplR"
                     /locus_tag="VGB88_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9646933.1"
                     /translation="MIKVKKLSERRTRRNRFHIRKAAAGRPRLSVFRSERHIYAQVIR
                     DDEGRTVAAASSLEAALRGQLKTGADVAAAKAVGKLIAERAIAAGVVQVVFDRGGYLY
                     HGRVRALADAAREGGLKL"
     gene            8346..8924
                     /gene="rpsE"
                     /locus_tag="VGB88_05515"
     CDS             8346..8924
                     /gene="rpsE"
                     /locus_tag="VGB88_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9646934.1"
                     /translation="MARTEGRRGGGRRDRDTRRQEAESDLFDKLVSINRVAKVVKGGR
                     RFGFAALVVVGDGRGRVGYGTGKAREVPEAIRKATEHAKRRLIRVPLREGRTLHHDIE
                     GHYGAGRVVLRAAPAGTGIIAGGPMRAVFETLGVADVVTKSIGTSNPHNMVRATFHAL
                     TRIQSPRAVAGKRGKKVGELVGRRDDAAAATE"
     gene            8944..9141
                     /gene="rpmD"
                     /locus_tag="VGB88_05520"
     CDS             8944..9141
                     /gene="rpmD"
                     /locus_tag="VGB88_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9646935.1"
                     /translation="MGAGAAGRVRVTQIGSPIGRHRIQRQILVGLGLNKMHRSRELED
                     TPAVRGMIAKVSHLVRVEDLP"
     gene            9138..9644
                     /gene="rplO"
                     /locus_tag="VGB88_05525"
     CDS             9138..9644
                     /gene="rplO"
                     /locus_tag="VGB88_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9646936.1"
                     /translation="MRLNQIQDKGGARRDRVRVGRGSASGKGRTAGRGMKGQKSRSGV
                     ALGGFEGGQMPLNRRLPKRGFRNTFSKTYATVNVGRLQQAVDAGKLDPKAPIDAGVLK
                     AAGVIRRPLDGVRLLGKGELKAKLTIEVAGASKGAVAAVEKAGGKVVITEPVEAPEAA
                     EAAAGDGD"
     gene            9682..11025
                     /gene="secY"
                     /locus_tag="VGB88_05530"
     CDS             9682..11025
                     /gene="secY"
                     /locus_tag="VGB88_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9646937.1"
                     /translation="MASAAEQLAAQFNFGALAKATELKKRLWFTLGALIIYRLGTYVP
                     IPGIDPAVLDDVFRQQAGGILGMFNMFAGGALERMTIFALNIMPYISASIMIQLLTTV
                     SPHLEQLKKEGESGRRKMTQYTRYGTVILATLQAFGIAVGLEGMTSSAGSAVIDPGLA
                     FRITTVITLVGGTVFLMWLGEQITARGVGNGISLIIMAGIVAQLPRSIASTLELGRTG
                     ALSATFIVILLVMAAAVVTFIVFIERAQRRLVVQYPKRQVGNRMFGGESSHLPLKLNT
                     AGVIPPIFASSLLLLPITALSFSAGQGPDWLTSVTAYLGRGQPLYLLIYVGLIVFFAY
                     FYTAVVFNPADTADNLRKHGGFIPGIRPGRNTAEYIDYVLTRLTTVGALYLAAVCVLP
                     EVLIARYAVPFYLGGTSLLIVVNVTMDFVAQVQSHLLAHQYEGLLRKSRLRGRRR"
     gene            11022..11678
                     /locus_tag="VGB88_05535"
     CDS             11022..11678
                     /locus_tag="VGB88_05535"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9646938.1"
                     /translation="MNLILFGPPGCGKGTQAERLHERLGLAHLATGDMLRAAVAAGTE
                     VGRRAQAIMAAGELVPDKLVVDIIAGRLDQADARDGFILDGFPRTLAQARALDDLLAE
                     KGRRIDRVVELEVDEDALVERISGRFACATCGAGYHDRFKPTKVEGVCDQCGGTDFVR
                     RQDDNAETVRERLGAYRRQTAPLLPYYRERGLLTSVDGMAGIDQVTAQVEAAVDGAGH
                     "
     gene            11767..12135
                     /gene="rpsM"
                     /locus_tag="VGB88_05540"
     CDS             11767..12135
                     /gene="rpsM"
                     /locus_tag="VGB88_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9646939.1"
                     /translation="MARIAGVNIPTNKRVQIALTYIYGIGRTKAREICAQAAIPSERR
                     VNDLTEGELARVRELIDGQYRVEGDLRRDTAMNVKRLMDMGCYRGLRHRRGLPVRGQR
                     THTNARTRKGRARPIAGKKK"
     gene            12196..12588
                     /gene="rpsK"
                     /locus_tag="VGB88_05545"
     CDS             12196..12588
                     /gene="rpsK"
                     /locus_tag="VGB88_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9646940.1"
                     /translation="MAKDTAGRLKRRERKNIISGVAHVNATFNNTLITISDVQGNTIA
                     WSSAGAQGFKGSRKSTPFAAQMAAEDAGRKAMEHGMKTLEVEVKGPGSGRESALRALQ
                     AVGFTITAIRDVTPIPHNGCRPPKRRRV"
     gene            12711..13727
                     /locus_tag="VGB88_05550"
     CDS             12711..13727
                     /locus_tag="VGB88_05550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9646941.1"
                     /translation="MIQKNWEELIKPSKLEIISGADPSRVATVVAEPLERGFGLTLGN
                     ALRRILLSSLRGSAITSLRIDNVLHEFSSIPGVHEDVTDIVLNIKSVAIRMHGDGPKR
                     VSLKATGPGAVTAGQIETGHDIEILSPEVVICTLDKGTSIHFEMTVESGKGYVAASQN
                     RPTDAPIGLMPVDALYSPVRKVSYKVENTRVGQVTDYDKLSMHVETDGTVKPEDAVAL
                     AARILTDQMNLFINFEEPERKTEEAEARQPAFNPNLLRKVDELELSVRSANCLKNDNI
                     IYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLGQLGLHLGMQIPEWPPENIEEMVKRL
                     EEPF"
     gene            13750..14184
                     /gene="rplQ"
                     /locus_tag="VGB88_05555"
     CDS             13750..14184
                     /gene="rplQ"
                     /locus_tag="VGB88_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX9646942.1"
                     /translation="MRHRMSGRKLGRKASHRKAMFSNLAAALIKHEQITTTLPKAKDL
                     RPVVERLITLGKRGTLHARRQALAKLGGDATLVDKLFTALSERYAERAGGYARVLRAG
                     FRYGDSAPMAVIELVDRDPAAKGQDSGPRPEAAAAAEEADAA"
     gene            14305..15729
                     /locus_tag="VGB88_05560"
     CDS             14305..15729
                     /locus_tag="VGB88_05560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX9646943.1"
                     /translation="MPRPRDPIIPLLAAGAVGLAALLAVPGAHAAERALPESRQAVQL
                     SYAPVVKQVAPAVVNIYTRRVVETGWRSPLFGDPLFERFFGQLFPGPPQERIQNSLGS
                     GVIVAGDGLIVTNNHVIEGAQEITVALADRREFEARLVGTDEASDLAVLRIDTGGEQL
                     PWLELGDSDAVEVGDIVLAIGNPFGVGQTVTSGIVSATARTAAIGRGGGAFIQTDAAI
                     NPGNSGGALVTLDGRLIGINTAIFSRSGGSIGIGFATPSNLVRVVVDTVVGGGRMVQP
                     WVGVDVQPVTADIARSLGLDRPAGVLIQAIHPASPAHDAGLRVGDVVLAIDGHEVFDT
                     DGFRFRLRMRASGATAVFTVLTRGGRREVEVKLIAPPETPPRDVRRLAGRSPLAGATV
                     ANLSPALADELDLDRNLTGVIVLDLEPGPAARLGLRPGDIVVEVAGVEIDEVATLARV
                     LQAERDEWTIAIRRNGEVRRVVVR"
     gene            15729..17054
                     /locus_tag="VGB88_05565"
     CDS             15729..17054
                     /locus_tag="VGB88_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9646944.1"
                     /translation="MATLFEAAGLDERVLRPLADRLRPQRLDQVVGQDHLLGPDGPIG
                     RMVAAHGLASIILWGPPGSGKTTIARLLADAVGLDFEPLSAVFSGVADLRKVFARAEQ
                     RRAAGRGTLLFVDEIHRFNRAQQDGFLPYVESGAVVLVGATTENPSFELNAALLSRCQ
                     VLVLNRLDRAALTELLARAEADAGRPLPLDGDAREALFGMADGDGRFLLNMAEAVYQL
                     PAGEALDTAALVRTVQRRAPVYDKAQEGHYNLISALHKSLRGSDADAALYWLARMLVA
                     GEDPLYIVRRLVRFASEDIGLADPQALVQALAAKDAYHFVGTPEGELAIAQAVLYLAT
                     APKSNAAYRALGAAVRAARATGSLMPPKHILNAPTRLMRELGYGAGYAYDHDTDDAFS
                     GQSYWPEGVEGERFYVPAERGFEREIAKRLKYWQDLRARLAAGDSDGDG"
     gene            17051..18082
                     /locus_tag="VGB88_05570"
     CDS             17051..18082
                     /locus_tag="VGB88_05570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9646945.1"
                     /translation="MTTLTTVEVAPDEAALRLDRWFQRHFPGLGRATLHKLLRTGQVR
                     VDGRRAAASLRLAPGQRVRVPPQAHGAPAPPRPAPAPAPTAAEVEALRARVLYRDDDI
                     IAIDKPAGLSVQGGSGQRRHLDALAAALQFDAAEPPRLVHRLDKDTSGVLVLARAARA
                     AARLAGLFRSGEVAKTYWAVVVGVPRPAAGVVTQALTKAGGPGREKVTAADGGGRPDS
                     AEAKPFRLRAGRPATTRYRTIARAGDRAAWLELRPLTGRTHQLRVHCLALGTPIAGDG
                     KYGGKAAALEIAPGKVVRRLQLHARELVIPQAPGGNLRLHAPVPAHMLATFRALGFDP
                     DSPAAEAGP"
     gene            18079..18780
                     /locus_tag="VGB88_05575"
     CDS             18079..18780
                     /locus_tag="VGB88_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9646946.1"
                     /translation="MTPPRAGSTPTLVIFDCDGTLVDSQDAIVRAMATAFAGAGRAAP
                     AAADVRRVVGLSLPEAMARLVPDGCGEDHAALAAHYREAFTAQHADGAPHGTLFPGAA
                     EAVARLDARGYLLGIATGKSRRGLATTLAGHGLEHLFATVQTADDAPSKPHPAMVAQA
                     IAATGARPETTVLIGDTAFDMAMALAAGAGALGVGWGYHPPEELRAAGAHRVVDSFDQ
                     IDDAVEALIALRRER"
     gene            18780..20327
                     /locus_tag="VGB88_05580"
     CDS             18780..20327
                     /locus_tag="VGB88_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9646947.1"
                     /translation="MRLRLWLMRGLALVVVVLCLAAVAIAGVIAAMPPEALAARALAQ
                     AAAALGRPIHAARAEVALWPAPLVSLHEVIAARAPGLARPGLLTARRVDIALAPWPLL
                     AGMVRIARIAIVQPRLTVDSELGALSSWHLDPIAGSGPVVTVADGSGMFRVRIGEGSL
                     AVALDRLDASWPGRGPVSVEAAGRIGAGTEVSLSGLWRARADGQTFDIAGGSLGGGAM
                     SGTVGLEPGCGRPRIAARLALGALALDGALRRLAGIDWGLDLDAADGDALVPLFSDRR
                     LALAGLRAVDLDLSLTAETASLGDVETQGARVKLSLSDGVLEVTRAEARLGDGQIALG
                     ARLDWIERRYRLQVDARDVPARLLGLGNPLDSLPGNLSLALDLEANGRSAHEIAASMT
                     GSLDLRLADAWLAGESSALVTAVFPWTRDRMGLVIEGAEARAQVVDGMIEAGAGRITT
                     PEVELALAGMVDLATESLYVDLVPSPRDTEAAGMMVPLIIAGPMAQPALLPNPAPIGA
                     EPDGDEP"
     gene            20324..21022
                     /locus_tag="VGB88_05585"
     CDS             20324..21022
                     /locus_tag="VGB88_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX9646948.1"
                     /translation="MRRRYRRVEVLTESAGFRVALDDRPLRTPAKHVLALPSRALAEA
                     VAGEWRAQETEILPATMPLTRLSNTAIDRIADRPGEAAAEIAAYAETDLLCYRADHPQ
                     ALARRQHLAWQPVLDWLAGRHHARLRVTHGVVPVAQDACALAALGRAVEAHDAFRLAA
                     LGAATRAAGSIALALALIEGEIDAETAAAASQLDERFQVEQWGDDDQASARRAALAAD
                     IASAAAFASLLAAG"
     gene            21126..22658
                     /locus_tag="VGB88_05590"
     CDS             21126..22658
                     /locus_tag="VGB88_05590"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9646949.1"
                     /translation="MDSIIRQLEERRAAARLGGGERRIETQHAKGKLTARERIELLVD
                     EGSFEEFDMFVEHRSTDFGMDRQRIPGDGVVTGHGTINGRKVFLFSQDFTVFGGSLSE
                     THAEKICKVMDLAMRVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVLASGVVPQI
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPDVVKTVTHEAVTAEDLGGAVSHS
                     TRSGVADLALDNDVEALAMARRLFDFLPLNNREPPPRIATDDPGGRTEPSLSSLVPPD
                     PHKPYDIKELILKVVDEGDFFELQPNYGANIVIGFARMEGRTVGIVANQPMVLAGCLD
                     IATARKAGRFVRFCDCFNIPILTFVDVPGFLPGTDQEYGGIITHGAKLLFAYAEATVP
                     KVTVIVRKAYGGAYDVMSSKHLRGDVNYAWPSAEIAVMGPKGAVEIIFRGSLADPEEV
                     ERKTEEYRAKFANPFIAASRGYIDDVILPPNTRPRICGALRLLLTKRLENPWKKHDNI
                     PL"
     gene            22853..24832
                     /locus_tag="VGB88_05595"
     CDS             22853..24832
                     /locus_tag="VGB88_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX9646950.1"
                     /translation="MFRKLLIANRGEIACRIIRTARRLGIGTVAVYSEADAGALHVRM
                     ADEAVAIGPAPSSDSYLALERVVDAAKRAGADAVHPGFGFLAENAAFARALAEAGIVF
                     VGPGPAAIAAMGDKLASKKLAERAGVNIIPGHAHAIADVDEAARVAAGLGYPVMIKAS
                     AGGGGKGMRAARDEAELRDGFDATRREARSAFGDDRILIERFIAEPRHVEIQVLADSY
                     GNAIHLNERECSIQRRHQKVIEEAPSPLVGAELRRVMGAQAVALARAVDYVSAGTVEF
                     VVGPDRQFYFLEMNTRLQVEHPVTEMTAGLDLVEQMIRIAAGERLAITQDQVPLQGWA
                     IEARVYAEDPSRGFLPSVGRLTRFIPPAEGAGVRVDAGVAEGDEITRFYDPMIAKLIV
                     HDASREAAIARMRRALDRFVVHGVRTNIAFLAAVLAHPRFADGRLSTGFIAEVFGAGY
                     DAAEAPAAVADVLAAVAAFADRRTAERAAAIGGLVPARECRLAIAIGAQRIDAAVSAI
                     EAGYRVALDSRILTVASDSWRPGLDLFEGRVDGREVAVQIRRGARGTWQLVHGGAEVE
                     VLVRGARAAELAARMPAKQAADLSKLLLSPMPGLMVSLAVADGDQVKAGEPLCVIDAM
                     KMENVLRAERDGTVAEIKVRPGDSVAVDQVLMTFE"
     gene            complement(24839..25327)
                     /locus_tag="VGB88_05600"
     CDS             complement(24839..25327)
                     /locus_tag="VGB88_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9646951.1"
                     /translation="MDFVALTEQFCAAATAGTGADLAALFAEDGVYHDGFYGAFHGRA
                     AIADMIDNHFRRDAEDFAWKMYDPALSDHVGYAHYVFAYTAKFNGAKGRRVVFEGIGR
                     FRLAAGLIAAYDEIFDAGIAMVQLGFAAERIVKRAERAAGILRTQTELARFMMPAPDA
                     GG"
     gene            25389..25682
                     /locus_tag="VGB88_05605"
     CDS             25389..25682
                     /locus_tag="VGB88_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9646952.1"
                     /translation="MALPVGHKAERVRISGRVQGVWFRGWTVDEARRRGLDGWVRNLR
                     DGSVEALFSGPAEAVDDMVAACRRGPPAARVDNLVRRAAAAVPGTGFTAKPTV"
     gene            complement(25691..25873)
                     /locus_tag="VGB88_05610"
     CDS             complement(25691..25873)
                     /locus_tag="VGB88_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX9646953.1"
                     /translation="MALEMRPACETCEAPLGHQADAFICSYECTFCAGCAGAMAFTCP
                     NCGGELVSRPRRRPKA"
     gene            complement(25885..26559)
                     /gene="lipB"
                     /locus_tag="VGB88_05615"
     CDS             complement(25885..26559)
                     /gene="lipB"
                     /locus_tag="VGB88_05615"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9646954.1"
                     /translation="MAPVRWTLSQAPVPYPAAVAEMEAEVAAIRAGRAAERVWLLEHP
                     PLYTRGASAKDSELLEPLRLPVHRTGRGGRFTYHGPGQRVGYVMLDLQALGRRADVRA
                     FVSGLEAWLIDTLADFGVRAGRRAGRIGVWVARPDGSEAKIGALGVRVRRWVTYHGVS
                     LNVAPELANFRGIVPCGLAGYGVTSLAALGVAATMAEVDARLRLQFERQFGATVDDAE
                     PARAAS"
     gene            26666..26752
                     /locus_tag="VGB88_05620"
     tRNA            26666..26752
                     /locus_tag="VGB88_05620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26700..26702,aa:Leu,seq:gag)
     gene            26944..28347
                     /gene="mgtE"
                     /locus_tag="VGB88_05625"
     CDS             26944..28347
                     /gene="mgtE"
                     /locus_tag="VGB88_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9646955.1"
                     /translation="MTDAPVDDARRPAAEDNGDPARPVTPEFVRDIAEALAAGRTADA
                     VERARTLHSADLADLVEALKPDERVALIGALGPHLDPEVLVDLDEHVRDLVLESIEPE
                     AIAAHAAELDTDDATHLIEDLDAADQQKVLEALPASERELIENALAYPENSAGRLMQR
                     DYVAVPSYWDVGQVIDWLRETDELPDEFYEIFVVDPRHRPVGTVPLNRAMRTKRPVSV
                     AEIMDPEPRLIPVDMDQEEVAYQFDQYGLASAAVVAPDGRLLGQITVDDVVDVIQEEA
                     EEDIHRLGGVAAQGDLYLSPLATVRRRWIWLLVNLLTAVIASLTIALFTDTIQQIVAL
                     AILMPIVASMGGNAGTQTLTVAVRALATRELTAANAARMLWKECVVGGTNGILFAIVT
                     GVVAALWFESPIIGVVIGLAMIVNLLVAGAAGMLIPLGLQRAGVDPAISGGIVLTTVT
                     DVVGFFAFLGLAKLIIL"
     gene            28587..29639
                     /locus_tag="VGB88_05630"
     CDS             28587..29639
                     /locus_tag="VGB88_05630"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9646956.1"
                     /translation="MADIRTRTVPGFEDVVLCDDADAGLKAVIAIHDTALGPALGGVR
                     MWRYENERAALDDALRLARGMTSKAAVAGLPLGGGKAVILGDPRRDKSEALFRAFGRM
                     VERLRGRYITAEDVGTSVEDIATVARETRHVAGLADGSGDPSPVTAAGTFHGIRAAAR
                     HRFGSDDVAGLRVAVQGVGHVGYHLCRLLHGAGADLVVSDVSATMVARAAVEFGAATV
                     APDAIYDADVDLFAPCALGGAINRDTVPRLKAAIVAGAANNQLAEPMDGRALHRRGIL
                     YAPDYVINAGGLINVSWDVLHRGEPYDRAEVLDQVARIDPVLTAIFARAAAEGRPANE
                     VADALARGRLRPAAAA"
     gene            complement(29725..30240)
                     /locus_tag="VGB88_05635"
     CDS             complement(29725..30240)
                     /locus_tag="VGB88_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9646957.1"
                     /translation="MHTMSKLVAAAGAAVVVLGLGAGAQALDQKPVISLEMANMMADA
                     CVAKAAASGWRPINVAVFDDGGNLKVFKRMEGAFLGSIQIAQMKGHSSTMFPFPSRVF
                     GELAFGKDGEPGRIPGIANIPGIASFPGGLPIMTAGGQQIGGIGVSGASADEDEMCAQ
                     AGIDAVTDMLK"
     gene            complement(30349..30792)
                     /locus_tag="VGB88_05640"
     CDS             complement(30349..30792)
                     /locus_tag="VGB88_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX9646958.1"
                     /translation="MTVHLLKMCVGAGSVDQLRALQGGRLARQAAAGEVPALRHWTRN
                     FPRRAGEIAGKGSLYWIIKGSVRVRQRILALERADDAAALKRCAIVLDPDLVETAPRR
                     CRPMQGWRYLEPADAPPDARPRGGDDAQLRMPAEMVEELRDLGLL"
     gene            31118..32038
                     /locus_tag="VGB88_05645"
     CDS             31118..32038
                     /locus_tag="VGB88_05645"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9646959.1"
                     /translation="MADDATRPRFAGVIPPVLTPFRADLSPACARFTAHARWLLDEGA
                     TGLAPFGTTGEANSLSVDERLVLLESLLGAGIDPAVVMPGTGCCALTDSVRLTEAAVR
                     AGCGGVLMLPPFYYKGVSEDGVFASIAEVVERVGDTRLHVYLYHIPPVAQVGFSLSLI
                     ERLIGAYPETVVGLKDSSGDWANTKAVIDAFPGFATFSGSEVFLLDNLRAGGAGCITA
                     TGNVNAAAIRALYDRWQGADADSLQADITALRKTIQAFPMIPALKAILARHLGDADWS
                     RTRPPLRDLTAEQARALTEALRTRAFALAA"
BASE COUNT         4865 a        11405 c        11614 g         4405 t
ORIGIN      
        1 aactaccggc cgcagttcta cttccggacc accgacgtga ccgggtcggt gaagctgccg
       61 gaggggacgg agatggtgat gccgggcgac aacatcgcga tggagatcga gctgatcgcg
      121 ccgatcgcga tggaccaggg cctgcggttc gcgatccgcg agggcgggcg cacggtcggc
      181 gccggcgtcg tcgccgagat cgtcgagtag cggcaggcgc gcgcggcaag gatcggacga
      241 tggaaagcca gaacatccgc attcgcctca aggcgtacga tcatcgggtg ctcgaccagt
      301 cgacgctcga gatcgtcaat accgccaagc gcaccggcgc ggcggtgcgc gggccgatcc
      361 cgctgccgac ccatatcgag cggttcacgg tgctgcgctc gccgcacatc gacaagaagt
      421 cgcgcgagca gttcgagatc cggacccaca agcggctgct cgacatcgtc gacccgacgc
      481 cgcagacggt cgacgcgctg atgaagctcg acctcgccgc cggcgtcgac gtcgagatca
      541 agctgtagcc gcgggccggg gccgaggaag ggatcggaca tgcgcaccgg agtgctggca
      601 cgcaagctcg gcatgtcccg cgtctttctc gacgacgggc ggcacctgcc ggtgaccgtg
      661 ctcgggatcg aggactgccg cgtggtggcg cagaagaccg cggagcgcga cggctacacc
      721 gcgctgcagg tcggcgccgg ccgggccaag cccaagaaca cctcgaaggc gatgcgcggg
      781 catttcgcca aggcgcgggt cgagcccaag ctcaggctgg ccgagttccg ggtgaccccg
      841 gactgcctgg tcgaggtcgg cgccgagctc ggcgccgggc atttcgtctc gggccagttc
      901 gtcgacgtgg ccggcacctc gatcggcaag ggcttcgccg gcgcgatgaa gcgccacggc
      961 ttccgcggcc tgggcgcgag ccacggcgtg tcgctcagcc atcgcgcgca cggctccacc
     1021 ggccagtgcc aggacccggg gcgggtgttc aagggcaagc ggatggccgg ccagatgggc
     1081 gccaagcggg tgaccgagca gaacctgtac gtggttgagg cggacgccgg gcgcggcctg
     1141 atcgtggtca tgggcggggt acccgggccg gtgggcgggt atctgcgcgt cagcgacgcc
     1201 aagaagatgg cgctgcccga gggcgtgccg atgccggccg gcgtgcgcgg cgcggcggtg
     1261 gcggccgagg tcactgaggg cgaggcgctg gccggcgacg ccggcggcac gcccgacgag
     1321 aaggactgag cgcggtgaag tgcgacgtga tcacgctgga taacggcaag gcgggcgaca
     1381 tcgagctcgc cgacgaggtg ttcgccgtac cggtgcgcgc cgacatcctg cagcgcgtgg
     1441 tggtctggca gctggccaag cgccgcgccg gcacccacaa ggtcaagggc cgcagcgagg
     1501 tgcgcgcgac cggggtcaag ttgtaccggc agaagggcac cggccgcgcc cgccacggct
     1561 cgcgcaaggt caacctgttc cgcggcggcg ggcgctcgtt cggacccgtg gtgcgcagcc
     1621 acgcgatcga gctgccgaag aaggtgcgcc ggctggcgct gaaatgcgcg ctggcgtcga
     1681 agcgtgccga cggcaagctg gtggtgctcg actcggcgac gctggaggcg ccgcgcaccg
     1741 ccgagcttgc ccgccgcatc gccgcgctcg gctggcactc ggcgctggtc atcgacggcg
     1801 ccgaggtcaa cgacaacttc cgccgcgcgg cgcgcaacct gcccggcctc gacgtgctgc
     1861 cgagccaggg cgccaacgtc tacgacatcc tgcgcagcga cctcttggtg ttgacccgcg
     1921 cggcagtggc ggcgcttgag gcgcgcctgc gatgaacgcc gaacgcgcct gcgacatcat
     1981 cctcggcccg gtgatcaccg agaaggcgac cgcgctcagc gagcacaacc agttcgcctt
     2041 ccgggtgcgc aaggacgcca ccaagggcga gatccgggcc gcgatcgagg ccctgttcgg
     2101 ggtcaaggtg accggcgtca acacgatcaa cgtcaagggc aaggtcaagc gcttccgcgg
     2161 ccacctgggc cggcgctcgg acgtcaagaa ggcgatcgtg accctcgccg agggccagag
     2221 cctcgactac acggcgggca tctgaggagc gggagagacg cgccatggca ctgaagagct
     2281 atcgcccgat cacgccgtcg cagcgccagc tggtgctggt cgaccgctcc gacctgtaca
     2341 agggcaagcc ggtcaagaag ctgaccgagg gcctgaccgg cagcggcggg cgcaacaatc
     2401 tcggccggat gaccgtgcgc catcgcggcg gcggccacaa gcggcgctac cggctgatcg
     2461 acctgaagcg ccgcaagctc ggcgtggcgg cggtcgtcga gcgcctggag tacgacccga
     2521 accgcagcgc gttcatcgcc ctgatccgct acgacgacgg cgagctcgcc tacatcctgg
     2581 cgccgcagcg cctgcgcccc ggcgacaagg tggtcgcgga ctcgcgcgcc gacatcaagc
     2641 ccggcaacgc catgccgatg aagaacatgc cggtcggcac gatcatccac aacgtcgaga
     2701 tgaagccggg caagggcggg cagatcgcgc gcgcggcggg caactatgcc cagctgatcg
     2761 gcaaggacgc gggctacgcg ctgctcaggc tgtcgtcggg cgagcagcgc atggtccatg
     2821 ccgagtgcat ggccacggtc ggcgcggtgt ccaacccgga caaccagaac atcaatttcg
     2881 gcaaggccgg gcgcatgcgc tggctgggcc ggcggcccaa ggtgcgcggc gtggccatga
     2941 acccggtcga ccatccccat ggcggcggcg agggccggac ctcgggcggg cgccacccgg
     3001 tgacgccgtg gggcgtgccg accaagggca agcgcacgcg caacaacaag gccaccgacc
     3061 ggctgatcct gcgcagccgt caccgggcca agaagaagaa gcggtaggga ccacagccat
     3121 ggcgcgttcc gtctggaaag ggccgttcgt cgacggctac ttgctcaaga aggccgaggt
     3181 cgtgcacggc tccgggcgca aggaggtgat caagacctgg tcacggcgct cgaccatcct
     3241 gccgcagttc gtcgggctca cctttggcgt gcacaacggc cacaagttca tcccggtgct
     3301 ggtgaccgag aacatggtcg gccacaagct gggcgagttc gcgccgactc gcacctatca
     3361 cggccactcc ggcgacaaga aggcgcgcag ggcctgagac catgggcaag cggacaccag
     3421 aacggcggct ggccgagaac gaggcgatgg cgatggcgcg cagcctgcgg accagcgcgc
     3481 gcaagctcaa cctggtcgcc gagtcgatcc gcgggctgtc ggcggagacg gcgctggcga
     3541 cgctcggctt ctcgcgccgg cgcatcgccc gcgacgtgcg caaggtgttg cagtcggcga
     3601 tcgccaacgc cgagaacaac cacaacctcg acgtcgaccg cctggtggtc gccgaggcct
     3661 tcgtcggcaa ggcgattacc atacgccgct ggcggccgcg cgcgcgcggg cgggtcgggc
     3721 gaatcgtcaa gccgttcagc cgcctgaccg tggtcgtgcg cgaacgagaa gaggactgag
     3781 ctgagcatgg gacagaaggt caatccgatc gggctgcgcc tcggcgtcaa tcgcacctgg
     3841 gactcgcgct ggtacgccga caacgactat gccgccctgc tgcacgagga cctgaagatc
     3901 cgcaacttcc tcgagcagcg gctggcccag gcgggcgtcg cccggatcgt gatcgagcgg
     3961 ccggccaaga aggcgcggat aaccatccac accgcccgcc cgggggtggt gatcggcaag
     4021 aagggcgccg acatcgagaa gctgcgcacc gacctgtcga agctcaccgc cagcgaggtg
     4081 gcgatcaaca tcgtcgagat ccgcaagccc gagatcgagg cccagctggt ggccgagaac
     4141 atcagccagc agctcgagcg gcgggtggcg tttcggcgcg cgatgaagcg cgcggtccag
     4201 tcggcgatgc gcctcggcgc gctcggcatc cgcatcacct gcgccgggcg cctcggcggc
     4261 gccgagatcg cgcgcaccga gtggtaccgc gagggccggg tgccgctgca cacgctcagg
     4321 gccgacgtcg attacggcac cgccaccgcg cgcaccgcct acggcacctg cggggtcaag
     4381 gtgtgggtgt tcaagggcga gatcatggag cacgacccga ccgcgcagga ccggcgccgg
     4441 gccgagcttc agggcgcgcg cgtctgagcg cggccggatc gaggaccgaa agccatgctg
     4501 agcccgaagc gaaccaagta ccgcaaggcc cacaagggcc gcgtccacgg cgccgccaag
     4561 ggcggcacca cgctcaactt cggctcctac gggctgaagg cctcggagcc ggcgcgcgtc
     4621 agcgcccgcc agatcgaggc ggccaggcgc gcgatcaccc gccacatcaa gcgcgccggg
     4681 cggatgtgga tccgcatctt ccccgacgtg ccggtgagca agaagccgac cgaggtgcgc
     4741 atgggcaagg gcaagggcgc gcccgagttc tggatggccc gtgtcaagcc gggtcggatc
     4801 atgttcgagc tcgacggcgt gccggcgcag ctcgcccgcg cggcgttcgc gcgcggcgcg
     4861 gcgaagctgc cgatgcgcag ccgcttcgtc gcccggatcg gcgaggaggc ctgaggcgat
     4921 gcgcgcgcgc gagctgattg ccaagggccg ggacgagctg tcggtccagc tccgcgacct
     4981 caagaaggag cagttcaacc tgcgctttca gcgggccacc ggccagctcg agaacaccgc
     5041 acggttccgc caggtgcgcc gcgacatcgc gcgcctcaag acggttctcg ggcaacaggc
     5101 ggccaagccc gcgcgcgagt cggagagcta gacgatgccc aggcgaatcc tgcggggaac
     5161 cgtgatctcg gacggcgccg acaagaccgt cgtggtgcgg gtcgagcggc gcttccgcca
     5221 cccgctgtac aagaagttca tccgcaagtc gaagaagtac gcggtgcacg acccggacaa
     5281 ccggcacaag gtcggcgacg tggtgcgcat ccgcgaatgc cggccggtgt cgaagtccaa
     5341 gcactgggaa gtgctgggcg agggcgcgta agcgcccgcc gccgcacgcg agaggacgcc
     5401 tgccatgatc cagatgcaga ccagcctgga cgttgccgac aattcgggcg cgcgacgggt
     5461 gcagtgcatc aaggtgctgg gcggaaccgg gcgcaagacc gcctcgatcg gcgacgtgat
     5521 cgtggtctcg gtcaaggagg cgatcccgcg cggccgggtc aagaagggcg acatccaccg
     5581 cgcggtgatc gtgcgcacgg ccaaggagat ccgccgcccc gacggcagcg cgatccgctt
     5641 cgaccgcaac gcggcggtgc tgatcacgcc gcagggcgag ccgatcggca cgcgcatctt
     5701 cggcccggtc acccgcgagc tcagggccaa gcggttcatg aagatcgtgt cgctggcgcc
     5761 cgaggtgctg tgatggcgaa caagctcaag atccggaagg gcgacaccgt cgtcgtgctg
     5821 tgcggcaagg aaaagggccg ccgcggcgag gtgctgcggg tgatccccga cctgcggcgg
     5881 gtggtggtgc gcggcctcaa catggtcaag cgccacaccc ggccgagcgc ggtcaccccg
     5941 ggcggcatcc tggagaagga ggcgccgctg cacgcctcga acctggcgct ggtcgatccc
     6001 aaggacggca agccgacccg ggtcggctac cgggtgctgg aggacggccg caaggtccgc
     6061 ttcgccaagc gctccggcga agtgatcgat cgttgacgga gacggcgatg gcgcgcttgc
     6121 gcgagcacta ccagaccacg gtcaagccgg cgctgatggc ccagttcggc tatcgcaacc
     6181 cgatgcaggt gccgaggctc gacaagatcg tggtcaacat gggcgtcggc gaggccgcgc
     6241 gcgacaacaa ggtgatccag ggggcggaag cggatctcgc cgcgctctcg ggccagaagc
     6301 cgctgatcac gcgcgcgcgc aagtcgatcg ccaacttcaa gctgcgcgag ggcatgaccg
     6361 tgggctgcaa ggtcacgctc aggcgcgagc gcatgtacga gttcctcgac cggctgatca
     6421 acatcgcgct gccgcgggtg cgcgacttcc acggcgtctc gccggccagt ttcgacggcc
     6481 gcggcaacta cgcgctcggc ctcaaggagc agatcgtgtt tcccgagatc gattacgacg
     6541 cggtcgacgc ggtgcgcggc ctcgacgtcg tgatcgtgac cactgccgcc accgacaacg
     6601 aggcccgcgc cctgctgcgc ggcttcaaca tgccgtttgt cggctgaggt cggagcggcc
     6661 cggtgacaac tggagagtga ccatatggcg aaacgaagcg caatcgaacg caacaacaag
     6721 cgcaagcgca tggtcgccag gtacgcggcc aagcgggcgg cgctgaaggc gatggccaat
     6781 gacgagtcgc tggcgatgga ggatcggttc gccgcccggc tcaagcttgc cgagcagccg
     6841 cgcaacggcg cgccgtcgcg ggttcgcaac cgctgcgagc tgtccggcag gccgcgcggc
     6901 tactaccgca agctcaagct gtcgcgcatc gccctgcgcg acctggcctc ggcgggccag
     6961 atccccggca tggtcaagtc gagctggtga ggagtccgcg gcgatgacga tgagcgaccc
     7021 cctcggcgac atgctcaccc gcatccgcaa cggccagcgc gtgcacaagg cgatcgtgcg
     7081 ctgcccggcg tcgaggctgc gcgtcaacgt gctcgaggtg ctgcgcaagg aaggctacat
     7141 ccgcggcttc cggcgcctcg acgaggaggc ggtgcagccg ctgctcgaga tcgagctcaa
     7201 gtaccaggag ggcacgccgg tgatccagga gatcagccgc gtctcgcgcc cgggccagcg
     7261 ggtctattcg tcgatcaggg acctggcgcg ggtgtacaac gggctcggca tctcgatcct
     7321 gtcgaccccg cgcggggtga tgtcggatgc cgacgcccgc gccgccaatg tcggcggcga
     7381 ggtgttgtgc aaggtgttct aggagagggc ggtgtcgcgc ataggcaagc acccggtctc
     7441 ggtgccgagc ggcgtcgagg tcgccatcgc cggccagacg ctgaccgtca agggcaagcg
     7501 cggcgcgctc agccgcacgc tcgccaacga ggttgaagtg cgccgcgacg gggaccagat
     7561 ctggatcaag ccgcgcgccg aggaccggcg ctcgcgcgcc atgtggggcc tgtcgcgatc
     7621 cttggtcaac aacatggttc tgggcgtgtc cgcgggcttc acgcgcatgc ttgagatctc
     7681 gggagtcggg ttccgggcgg cggtggacgg gcgctatctc aacctgcagc tcggctacag
     7741 ccacgaggtc aagctggcgg tgccgccgga cgtggagatc acctgcccgc gcccgaccgc
     7801 gatcacgatc accggcgccg accggcagcg ggtcggtcag atcgcggccg agatccgcgc
     7861 gctgcgcaag cccgagccgt acaagggcaa gggcatccgc tacgccgagg agcacgtcag
     7921 gcgcaaggaa ggcaagaaga agtaaccggt ccaggcgatg atcaaggtca agaagctctc
     7981 ggaacgacgc acgcgtcgca accgcttcca catccgcaag gccgcggccg ggcggccgcg
     8041 gctgtcggtg ttccgctccg aacggcacat ctacgcccag gtcatccgcg acgacgaggg
     8101 gcgcacggtg gcggccgcct cgtcgctgga ggcggcgttg cgcggccagc tcaagaccgg
     8161 cgccgacgtc gccgcggcga aggcggtcgg caagctgatc gccgagcgcg cgatcgccgc
     8221 cggcgtcgtg caggtggtgt tcgaccgcgg cggctatctc tatcacgggc gggtgcgggc
     8281 actcgccgat gccgcgcgcg agggcggcct gaaattgtga acatgaacct gacgggacgc
     8341 gtctgatggc gaggactgag ggccgacgcg gtggtggccg ccgcgatcgc gatacgcgac
     8401 gacaagaggc cgaaagcgac ctgttcgaca agctggtcag catcaatcgg gtcgccaagg
     8461 tggtcaaggg cgggcgccgg ttcggcttcg ccgcgctggt ggtggtcggc gacggccgcg
     8521 gccgggtcgg ctacggcacc ggcaaggcac gcgaggtgcc cgaggcgatc cgcaaggcga
     8581 ccgagcacgc caagcggcgg ctgatccggg tgccgttgcg cgagggccgc acgctgcacc
     8641 acgacatcga gggccactac ggcgccgggc gggtggtgct gcgggcagcg ccggcgggca
     8701 ccggcatcat cgccggcggc ccgatgcgcg ctgtgttcga gacccttggc gtcgccgacg
     8761 tggtcaccaa gtcgatcggc acctccaacc cgcacaacat ggtgcgcgcg accttccacg
     8821 cgctcacgcg catccagtcg ccgcgggcgg tggccggcaa gcgcggcaag aaggtcggcg
     8881 agctggtcgg gcggcgcgac gacgcggccg cggcgacgga atagggcgat ggcgaccaag
     8941 gccatgggcg ccggcgccgc cggccgggtg cgcgtcaccc agatcggcag cccgatcggc
     9001 cgccaccgca tccagcgcca gatcctggtc ggcctcggac tgaacaagat gcaccgcagc
     9061 cgcgagctcg aggacacccc ggcggtgcgc ggcatgatcg cgaaagtcag tcacctggtg
     9121 cgcgtcgagg acctgccatg aggctcaacc agattcagga caaggggggg gcgcgccgcg
     9181 accgcgtccg ggtcggccgc ggcagcgcct cgggcaaggg acggaccgcc gggcgcggca
     9241 tgaagggcca gaagtcacgc agcggcgtcg ccctcggcgg cttcgagggc ggccagatgc
     9301 cgctcaaccg ccggctgccg aagcgcgggt ttcgcaacac cttttccaag acctacgcca
     9361 cggtcaacgt cgggcgcttg caacaggcgg tcgacgccgg caagctcgat cccaaggcgc
     9421 cgatcgacgc cggcgtgctc aaggcggcgg gcgtgatcag gcggccgctc gacggggtgc
     9481 gcctgctcgg caagggcgag ctcaaggcca agcttacgat cgaggtcgcc ggcgcatcga
     9541 aaggcgccgt cgcggcggtc gagaaggcgg gcggcaaggt ggtgataacc gagcccgtcg
     9601 aggcccctga ggccgccgag gccgccgccg gcgacggcga ctgaacgccg cgcaaaccgg
     9661 tcgcgccgct cgaggaagcc aatggcatca gccgcagagc aactcgccgc ccagttcaac
     9721 ttcggcgccc tggccaaggc gaccgagctc aagaagcgcc tgtggttcac cctcggcgct
     9781 ctgatcatct accggctggg cacctacgtc ccgatcccgg gaatcgaccc ggcggtcctc
     9841 gacgacgtgt tccgccagca ggccggcggc atcctgggca tgttcaacat gttcgccggc
     9901 ggcgcgctgg agcggatgac catcttcgcg ctgaacatca tgccgtacat ctcggcatcg
     9961 atcatgatcc agctgctgac tacggtctcg ccgcatctgg agcagctgaa gaaggagggc
    10021 gagtccggcc ggcgcaagat gacccagtac acgcgctacg gcacggtcat cctggcgacg
    10081 ctgcaggcgt tcggcatcgc ggtcgggctc gagggcatga cctcgagcgc gggcagcgcg
    10141 gtgatcgatc ccggcctcgc gttccgcatc accaccgtca tcaccctggt cgggggcacc
    10201 gtcttcctga tgtggctggg cgagcagatc accgcccgcg gggtgggcaa cggcatctcg
    10261 ctgatcatca tggccgggat cgtcgcccag ctgccgcgct cgatcgccag cacgctcgag
    10321 ctggggcgca ccggcgcgct gtcggcgacc ttcatcgtca tcctgctggt gatggcggcc
    10381 gccgtggtca cgttcatcgt gttcatcgag cgcgcccagc gccggctggt ggtccagtac
    10441 ccgaaacgcc aggtcggcaa ccgcatgttc ggcggcgaga gctcgcatct gccgctcaag
    10501 ctcaacaccg cgggcgtgat cccgccgatc ttcgccagct cgctcctgct gctgccgatc
    10561 accgcgctca gcttctcggc cgggcagggg ccggactggc tgacctcggt caccgcctat
    10621 ctcgggcgcg gccagccgct ctatctgctg atctatgtcg ggctgatcgt gttcttcgcc
    10681 tatttctaca ctgcggtggt gttcaacccg gcggataccg ccgacaacct gcgcaagcac
    10741 ggcggcttca tcccgggcat ccggccggga cgcaacaccg ccgagtacat cgactacgtg
    10801 ctgacccgcc tcacaacggt cggcgcgctg tatctcgcgg cggtgtgcgt gctgcccgag
    10861 gtgctgatcg cgcgctacgc ggtgccgttc tacctcggcg gcaccagtct gctgatcgtg
    10921 gtcaacgtga ccatggactt cgtcgcccag gtgcagtcgc acctcctggc ccaccagtat
    10981 gaggggctgc tgcgcaagag ccggctcagg ggccggcgac gatgaacctg atcctgttcg
    11041 gcccgcccgg ctgcggcaag ggcacccagg ccgagcggct gcatgagcgg ctggggctcg
    11101 cgcatctcgc caccggcgac atgctgcgcg ccgcggtcgc cgccggcacc gaggtcggcc
    11161 ggcgcgcgca ggcgatcatg gcggccggcg agctggtgcc cgacaaattg gtggtcgaca
    11221 tcatcgccgg gcggctcgac caggcggacg cgcgcgacgg cttcatcctc gacggcttcc
    11281 cccgcaccct cgcgcaggcg cgggcgctgg acgacctgct ggcggaaaag ggccggcgca
    11341 tcgaccgggt ggtcgagctc gaggtcgacg aggacgccct ggtcgagcgc atctcgggcc
    11401 gcttcgcctg cgccacctgc ggcgccggct atcacgaccg cttcaagccg accaaggtcg
    11461 agggggtgtg cgaccagtgc gggggcaccg acttcgtccg ccgccaggac gacaacgccg
    11521 agaccgtgcg cgagcgcctg ggggcgtacc gccgccagac cgcgccgctg ctcccctact
    11581 accgcgagcg ggggctcttg acctcggtcg acggcatggc cgggatcgac caggtgaccg
    11641 cccaggtcga ggcagcggtc gacggggccg ggcattgact tcgcatcccg ccgacctata
    11701 atcggcgacc ctcgagatta cgccgtttcg ccagcagcgg cgtcattttt ttcaggagct
    11761 taggccgtgg cgcgcatcgc gggtgtcaac atcccgacca acaagcgggt gcagatcgca
    11821 cttacctaca tctacggtat cggtcggacc aaggcccgcg agatctgcgc ccaggccgcg
    11881 attccgtcgg agcgccgggt caacgacctg accgagggcg aactggcgcg ggtgcgcgag
    11941 ctgatcgacg gccagtaccg ggtcgagggc gacctgcgcc gggacacggc gatgaacgtc
    12001 aagcgcctga tggacatggg ctgctaccgc gggctcaggc atcgccgcgg cctgccggtc
    12061 cgcggccagc gcacgcacac caacgcgcgc acccgcaagg gccgggcgcg gccgatcgcc
    12121 ggcaagaaga agtaggctcg gctgcgggct tgcgttcgcc ggcaggcggc acgaaatcag
    12181 gactgaaggc aaaggatggc aaaggatacg gccggtcggc tcaaacgccg cgagcgcaag
    12241 aacatcatct cgggcgtggc gcacgtcaac gcgacattca acaacacctt gatcaccatc
    12301 agcgacgtcc agggcaacac catcgcctgg tcgtcggcgg gtgcgcaggg cttcaagggc
    12361 tcgcgcaagt cgaccccgtt cgccgcccag atggcggccg aggacgccgg tcgcaaggcg
    12421 atggagcacg gcatgaagac gctcgaggtg gaggtcaagg gaccggggtc gggacgcgaa
    12481 tcggcgttgc gcgcactcca ggcggtcggc ttcacgatca ccgcgatccg ggacgtgacc
    12541 ccgatcccgc acaacggctg ccggccgccc aagcgacgtc gcgtgtgacg cgacccgcgg
    12601 ccggggcgcc gccgccggag tgccgtggcg cggcaggcgg cgggcgcctc gattcatagt
    12661 tttcgcgtcg gcgccgacgg gcgccggcag gtagtcgtga ggttgtggta gtgatccaga
    12721 agaactggga agagctgatc aagccgtcca agctcgagat catctcgggc gcggatccga
    12781 gccgggtcgc gaccgtggtc gccgagccgc tcgagcgcgg cttcgggctg acgctcggca
    12841 acgcgctgcg ccggatattg ctgtcgagcc tgcgcggctc ggcgatcacg tccttgcgca
    12901 tcgacaacgt gctgcacgag ttctcgtcga ttcccggcgt gcacgaggac gttaccgaca
    12961 tcgtgctcaa catcaagtcg gtggcgatca ggatgcacgg cgacggcccg aagcgggtat
    13021 cgctcaaggc gaccggcccc ggggcggtga ccgccggcca gatcgagacc ggccacgaca
    13081 tcgagatcct gtcgcccgag gtggtgatct gcaccctcga caagggcacc tcgatccact
    13141 tcgagatgac ggtcgagagc ggcaagggct acgtcgccgc cagccagaac cgccccaccg
    13201 acgcgcccat cgggctgatg ccggtcgacg cgctctacag ccccgtgcgc aaggtctcct
    13261 acaaggtcga gaacacgcgc gtcggccagg tcaccgacta cgacaagctg tcgatgcatg
    13321 tcgagaccga cggcacggtc aagcccgagg acgcggtcgc gctggcggcg cgcatcctca
    13381 ccgaccagat gaacctgttc atcaacttcg aggagcccga gcgcaagacc gaggaggcgg
    13441 aggcgcgcca gccggcgttc aatcccaacc tgctgcgcaa ggtcgacgag ctcgagctgt
    13501 cggtgcgctc ggccaactgc ctcaagaacg acaacatcat ctatatcggc gacctggtcc
    13561 agaagaccga ggccgagatg ctgcgcacgc ccaacttcgg gcgcaagtcg ctcaacgaga
    13621 tcaaggaggt cctcggccag ctcggcctgc acctgggcat gcagatcccc gagtggccgc
    13681 ccgagaacat cgaggagatg gtcaagcggc tcgaggagcc gttctgaccg cgacccgagg
    13741 gagagcccca tgcgtcatcg gatgagcggg cgcaagctcg gccgcaaagc cagccaccgc
    13801 aaggccatgt tttcgaacct ggccgcggcg ctgatcaagc acgagcagat cacgaccacc
    13861 ctgcccaagg ccaaggacct caggccggtg gtcgagcgcc tgatcacgct cggcaagcgc
    13921 ggcacgctgc acgcccgccg ccaggcgctg gccaagctcg gcggcgacgc cacgctggtc
    13981 gacaagctgt ttaccgcgct cagcgagcgc tacgccgagc gtgccggcgg ctatgcccgg
    14041 gtgctgcgcg caggcttccg ctacggcgac tcggcgccga tggcggtgat cgagctggtc
    14101 gaccgcgacc ccgccgccaa ggggcaggac tcgggcccgc gtcccgaggc cgcagcggcc
    14161 gcggaggagg ccgacgccgc ttaagccttg gcccagcgcc cgcgccgccg aagccttacc
    14221 gccggttggt tttttgcgcc accggcatga gcggcggctc cgctacaatc cgggccggcc
    14281 gccgcggcat gtcccggaac ctcgatgcct cgacctcgtg acccgatcat ccccctgctc
    14341 gcggccggcg ccgtcggcct ggcggccctg ctcgccgtgc cgggcgcgca cgcggccgag
    14401 cgcgcgctgc ccgagtcgcg ccaggcggtg cagctgtcct atgcgccggt ggtcaagcag
    14461 gtggcgccgg cggtggtcaa catctacacc cgccgcgtgg tcgagaccgg ctggcgcagc
    14521 ccgctgttcg gcgaccccct gttcgagcgg ttcttcggcc agctgtttcc gggcccgccg
    14581 caggagcgca tccagaactc gctcggctcg ggcgtgatcg tcgccggcga cgggctgatc
    14641 gtgaccaaca accacgtcat cgagggggcc caggagatca cggtggcgct cgccgaccgg
    14701 cgcgagttcg aggcgcgcct ggtcggcacc gacgaggcga gcgacctcgc cgtgctgcgc
    14761 atcgataccg gcggcgagca gctgccctgg ctcgagctcg gcgattccga cgcggtcgag
    14821 gtcggcgaca tcgtgcttgc gatcggcaac ccgttcggcg tcggccagac cgtgaccagc
    14881 ggcatcgtct cggcgaccgc gcgcaccgcc gccatcggcc gcggcggcgg cgccttcatc
    14941 cagaccgacg ccgcgatcaa tccgggcaac tcgggcggcg cgctggtcac cctcgacggc
    15001 cgcctgatcg gcatcaacac cgcaatcttc tcgcgctcag gcggctcgat cggcatcggc
    15061 ttcgccaccc cgtccaacct ggtgcgggtg gtggtcgaca ccgtggtcgg cggcgggcgg
    15121 atggtgcagc cctgggtcgg cgtcgatgtg cagccggtga ccgccgacat cgcccgctcg
    15181 ctcggcctcg accggccggc cggggtgctg atccaggcga tccacccggc gagcccggcg
    15241 cacgacgccg gcctcagggt cggcgacgtg gtgctggcga tcgacggcca cgaggtgttc
    15301 gacaccgacg gcttccgctt ccgcctgcgc atgcgcgcct cgggcgcgac ggcggtgttc
    15361 accgtgctca cccgcggcgg ccggcgcgag gtcgaggtga agctgatcgc cccgcccgag
    15421 accccgccgc gcgacgtccg ccgcctcgcc gggcgcagcc cgctcgccgg cgccaccgtc
    15481 gccaacctgt cgccggcgct ggccgacgag ctggacctcg accgcaacct gaccggtgtg
    15541 atcgtgctcg acctcgagcc cgggccggcg gcgcgcctgg gcctgcgccc gggcgacatc
    15601 gtggtcgagg tcgccggggt cgagatcgac gaggtggcga cgctggcccg ggtgctgcag
    15661 gcggagcggg acgaatggac catcgccatc cgccgcaacg gcgaggtccg ccgcgtggtg
    15721 gtgcgctgat ggcgaccctg ttcgaggcgg cggggctgga cgagcgggtg ctgcggccgc
    15781 tcgccgaccg gctgcgcccc cagcgcctcg accaggtggt cggccaggac cacctgctcg
    15841 gcccggacgg gccgatcggg cgcatggtcg ccgcccacgg cctcgcctcg atcatcctgt
    15901 gggggccacc cggcagcggc aagaccacca tcgcgcgcct gctcgccgac gccgtcggcc
    15961 tcgacttcga gccgctgtcg gcggtcttct cgggcgtcgc cgacctgcgc aaggtgttcg
    16021 cccgcgccga gcaacggcgc gccgccgggc gcggcacgct gctgttcgtc gacgagatcc
    16081 accgcttcaa ccgcgcccag caggacggct tcctgcccta cgtcgaatcg ggcgccgtgg
    16141 tgctggtcgg cgcgaccacc gagaacccgt ccttcgagct caacgccgcg ctcttgtcgc
    16201 gctgccaggt gctggtgctc aaccggctcg accgggcggc gctgaccgag ctgctggcgc
    16261 gcgccgaggc cgacgccggc cggccgctgc cgctcgacgg cgacgcgcgc gaggcgttgt
    16321 tcggcatggc cgacggcgac ggccgcttcc tgctcaacat ggccgaggcg gtctaccagc
    16381 tgccggcggg cgaggccctc gacaccgccg ccctggtgcg caccgtccag cggcgcgcgc
    16441 cggtctacga caaggcccag gaaggccact acaacctgat cagcgcgctg cacaagagcc
    16501 tcaggggctc ggacgccgac gccgcgctgt attggctcgc gcgcatgctg gtcgcgggcg
    16561 aggacccgct gtacatcgtc cgccggctgg tgcgcttcgc ctccgaggac atcggcctcg
    16621 ccgacccgca ggcgctggtc caggcgctgg cggcgaagga cgcctatcac ttcgtcggca
    16681 cgcccgaggg cgagctcgcg atcgcccagg cggtgctgta tctggccacc gcgcccaagt
    16741 cgaacgccgc ctatcgcgcg ctcggcgctg ccgtgcgcgc ggcgcgcgcg accggctcgc
    16801 tgatgccgcc gaagcacatc ctgaacgcgc ctacccggct gatgcgggag ctcggctacg
    16861 gcgcgggcta cgcctacgac cacgacaccg atgacgcgtt ctccggccag agctattggc
    16921 cggagggcgt cgagggcgag cggttctatg tcccggccga gcgcggcttc gagcgcgaga
    16981 tcgccaagcg cttgaagtat tggcaagacc tgcgcgcccg gctcgccgcc ggcgacagtg
    17041 acggcgacgg atgaccaccc tgacgacggt cgaggtcgcg cccgacgagg cggcattgcg
    17101 cctcgaccgt tggttccagc gccacttccc gggcctcggc cgcgcgacct tgcacaagct
    17161 cctgcgcacc ggccaggtgc gggtcgacgg ccggcgcgcg gccgccagcc tgcgcctcgc
    17221 ccccggccag cgggtgcggg tgccgcccca ggcgcacggc gcgccggcgc cgccgcgccc
    17281 ggcgccggcg ccggcgccga cggcggccga ggtcgaggcg ttgcgcgcgc gcgtgctgta
    17341 ccgcgacgac gacatcatcg ccatcgacaa gccggccggg ctctcggtcc agggcggcag
    17401 cggccagcgc cgccatctcg atgcgctcgc cgccgcgctc cagttcgacg ccgccgagcc
    17461 gccgcgcctg gtccaccggc tggacaagga caccagcggc gtactggtgc tggcccgggc
    17521 tgcgcgcgcg gcggcgcggc tggccggcct gttccggagt ggcgaggtgg ccaagaccta
    17581 ctgggcggtg gtggtcggcg tgccgcggcc ggccgcgggc gtggtcaccc aggcgctaac
    17641 caaggccggc gggccggggc gcgagaaggt gacagcggcc gacggcggcg gccggcctga
    17701 ttccgccgaa gcgaagccgt ttcggcttcg cgcaggcagg ccggcgacga cccgctaccg
    17761 caccatcgcc cgcgccggcg accgcgccgc gtggctcgag ctcaggccgc tgaccgggcg
    17821 cacccatcag ctgcgcgtcc attgcctggc gctcggcacc ccgatcgccg gcgacggcaa
    17881 gtatggcggc aaggccgcgg cgctcgagat cgcgccgggc aaggtagttc gccgcctgca
    17941 gctgcacgcg cgcgagctgg tgatcccgca ggcgccgggc ggcaacctgc gcctgcacgc
    18001 gccggtgccc gcgcatatgc tggccacgtt ccgggcgctc ggcttcgatc ccgactcgcc
    18061 ggcggcggag gccgggccgt gaccccgccg cgcgctggct cgacgccgac gctggtgatc
    18121 ttcgactgcg acggcaccct ggtcgatagc caggacgcga tcgtccgcgc catggcgacc
    18181 gcgtttgccg gcgccggacg ggcggcgccc gcagcagccg acgtccgccg cgtggtcggg
    18241 ctgtcgttgc ccgaggcgat ggcgagactg gtcccggacg gatgcggcga ggatcacgcc
    18301 gcgctggccg cgcactatcg cgaggcgttc accgcccagc acgccgacgg cgcgccgcac
    18361 ggcaccctgt tccccggcgc ggccgaggcg gtggcccgcc tcgatgcgcg cggctacctg
    18421 ctcggcatcg ccaccggcaa gtcgcggcgc gggcttgcca ccaccctcgc cggtcacggc
    18481 ctcgagcacc tgttcgccac cgtccagacc gccgacgacg cgcccagcaa gccgcacccg
    18541 gcgatggtcg cccaggcgat cgccgcgacc ggggcgcggc ccgagaccac ggtcctgatc
    18601 ggcgataccg cgttcgacat ggcgatggcg ctcgccgccg gcgccggcgc gctcggggtc
    18661 ggctggggct atcacccgcc cgaggagctg cgcgccgcgg gcgcgcaccg ggtggtcgac
    18721 agcttcgacc agatcgacga cgcggtcgag gcattgatcg ccctgcgccg ggagcggtga
    18781 tgcgcctgcg tctatggctc atgcgcgggc tggcgctggt cgtcgtggtg ctgtgcctcg
    18841 ccgcggtcgc gatcgccggc gtgatcgccg cgatgccgcc cgaggcactc gccgcccgcg
    18901 cgctggcgca ggcggcggcg gcgctcgggc gaccgatcca cgccgcccgc gctgaggtgg
    18961 cgctgtggcc ggcgccgctg gtcagcttgc acgaggtgat cgcggcgcgc gcaccgggcc
    19021 tggcgcggcc cggcctgctg accgcgcgcc gcgtcgacat cgcgctcgcg ccgtggcctc
    19081 tgcttgccgg gatggtacgg atcgcgcgca tcgccatcgt ccagccgcgg ttgaccgtcg
    19141 acagcgagct gggcgcgctg tcgtcctggc atctcgatcc aatcgccggc agcggccccg
    19201 tggtcacggt cgcggacggc tcgggcatgt tccgggtgcg catcggcgag ggctcgctgg
    19261 cggtggcgct cgatcggctg gacgcgagct ggcccggacg cgggccggtg tcggtcgagg
    19321 ccgcgggccg catcggcgcc ggcaccgagg tctcgctcag cggcctgtgg cgcgcgcgcg
    19381 ccgacggcca gactttcgat attgccggcg gcagcctcgg cggcggggcc atgtcgggga
    19441 ccgtcggcct ggagcccggc tgcggccggc cgcgaattgc cgcccggctc gcgctcggcg
    19501 cgctcgcgct cgacggcgcg ctccggcgct tggcggggat cgattgggga ctggacctcg
    19561 acgccgccga cggcgatgcg ttggtgccgc tgttctctga ccgccggctc gccctcgccg
    19621 gcttgcgcgc ggtcgatctc gatctttcgc tgaccgcgga aacggcctcg ctcggggacg
    19681 tcgagacaca aggcgcgcgc gtgaagctgt cgctgtccga cggggtgctc gaggtcacgc
    19741 gcgccgaggc ccgccttggc gacggccaga tcgcgctcgg cgcccggctt gactggatcg
    19801 agcgccgcta ccggctgcag gtcgacgcgc gggacgtgcc tgcgcgcctg ctcggcctcg
    19861 gcaatccgct cgacagtttg ccgggcaatc tttcgctggc gcttgatctg gaggccaacg
    19921 ggcgcagcgc gcacgagatc gcggcctcga tgaccgggtc gctcgacctg cgcctcgccg
    19981 acgcgtggct ggccggggag tcctcggcgc tggtgaccgc ggtgttcccc tggacgcgcg
    20041 atcggatggg cctggtgatc gaaggcgccg aggcgcgcgc gcaggtggtc gacggcatga
    20101 tcgaggcggg cgccgggcgg atcaccacgc ccgaggtcga gcttgcgctc gcgggcatgg
    20161 tcgatctcgc caccgaaagc ctctatgtcg acctggtacc gagcccgcgc gacaccgagg
    20221 ccgcgggcat gatggtgccg ctgatcatcg ccggcccgat ggcgcagccg gcgctgctgc
    20281 ccaacccggc ccccatcggc gccgagcccg acggggacga gccgtgagac gccgctatcg
    20341 gcgcgtggag gtgcttacgg agtcggcggg gttccgggtg gcgctcgacg accgcccgct
    20401 caggaccccg gccaagcacg tgctggccct gccgagccgc gcgctggccg aggccgtggc
    20461 cggggagtgg cgggcgcaag agaccgagat cctgccggcg acgatgccgc tcacgcggct
    20521 gtcgaatacc gccatcgacc gcatcgccga ccgccctggc gaggcggcgg cggagatcgc
    20581 cgcctacgcc gagaccgacc tgctgtgcta tcgcgccgac cacccgcagg cgcttgcccg
    20641 ccgccagcac ctggcctggc agccggtgct cgattggctg gccgggcgcc accacgcgcg
    20701 cctgcgcgtc acccacggcg tggtgccggt ggcccaggat gcgtgcgcgc tcgccgcgct
    20761 cgggcgcgcg gtcgaggccc atgacgcgtt ccgtcttgcg gcgctcggcg cggccacccg
    20821 ggcggcgggc tcgatcgcgc tcgccctggc attgatcgag ggcgagatcg acgccgagac
    20881 cgcggccgcg gcgagccagc tcgacgagcg cttccaggtt gagcagtggg gcgacgacga
    20941 ccaggcatcg gcgcggcgcg cggcgcttgc cgccgacatc gcgtcagcgg cggccttcgc
    21001 ctcgctactg gcggcgggtt gagccggcgg cgccccgcgc tggcgtcgaa tcggccgaga
    21061 tgttatgtgc aggtgcccgc ccgcggccgc gagcggccgt cctgcgggcc cgtcgcgagg
    21121 gggccatgga ttcgatcatc cgacagcttg aggagcgccg tgccgcggcg cgtctgggcg
    21181 gcggcgagcg ccgcatcgag acccagcacg ccaagggcaa gctgaccgca cgcgagcgaa
    21241 tcgagctgct ggtcgatgag ggctcgttcg aggagttcga catgttcgtc gagcaccgca
    21301 gcaccgattt cggcatggac cgccagcgca tcccgggcga cggcgtggtc accggccacg
    21361 gcaccatcaa cggccgcaag gtgttcctct tcagccagga cttcaccgtg ttcggcggct
    21421 cgctgagcga gacgcatgcc gagaagatct gcaaggtcat ggatctggcg atgcgcgtcg
    21481 gcgcaccggt gatcgggctc aacgattcgg gcggcgctcg catccaggag ggcgtcgcct
    21541 cgctcggcgg ctacgccgag gtgtttcagc gcaacgtgct cgcctcgggc gtggtgccgc
    21601 agatcagcct gatcatgggg ccgtgcgcgg gcggcgcggt ctactcgccg gcgatgaccg
    21661 acttcatctt catggtcaag gactcgtcct acatgttcgt caccggcccc gacgtggtca
    21721 agacggtgac ccacgaggcg gtgaccgccg aggacctggg cggcgcggtc agccactcga
    21781 cccggtccgg cgtcgccgac ctcgcgctcg acaacgacgt cgaggcgctg gcgatggcgc
    21841 gccggctgtt cgacttcctg ccgctcaaca atcgcgagcc gccgccgcgg atcgccaccg
    21901 acgaccccgg cgggcgcacc gagccgtcgc tctcgagcct ggtgccgccg gacccgcaca
    21961 agccttacga catcaaggag ctgatcctga aggtggtcga cgagggcgac ttcttcgagc
    22021 tgcagccgaa ttacggcgcc aacatcgtga tcggtttcgc gcgcatggag ggacgcacgg
    22081 tcggcatcgt cgccaaccag ccgatggtgc tcgccggttg cctcgacatc gccaccgcgc
    22141 gcaaggccgg ccgcttcgtc cgcttctgcg actgcttcaa catcccgatc ctgaccttcg
    22201 tcgacgtgcc cggcttcctg cccggcaccg accaggaata cggcggcatc atcacccacg
    22261 gcgccaagct gctgttcgcc tatgccgagg cgacggtgcc caaggtcacc gtgatcgtgc
    22321 gcaaggccta tggcggcgcc tacgacgtga tgagctcgaa gcatttgcgc ggcgacgtca
    22381 actatgcctg gccgagcgcc gagattgcgg tgatggggcc gaagggcgcg gtcgagatca
    22441 tcttccgcgg ctcgctcgcc gaccccgagg aggtcgagcg caagaccgag gagtatcgcg
    22501 ccaagttcgc caaccctttc attgccgcaa gccgcggcta tatcgatgat gtgatcctgc
    22561 ctccgaacac ccgcccgcgc atctgcgggg cgctcaggct gctgctcacc aagcggctcg
    22621 agaacccgtg gaagaagcat gacaacatcc cgctctaatt tcccggtact tacgatcgca
    22681 ggagaccgag tcgcgaacct catccttcga gacgcgccct cccgccttcg cgaagcccgc
    22741 ttccaccctc cgcagcggca gcggcgccgc cgctgctgcg gaggacgggt cggcgggcga
    22801 agcaagggcg ggcgctcctc aggctgagga cgtctcgtag cgcccgccct ccgtgttccg
    22861 caagctgctg atcgccaatc gcggcgagat cgcctgccgc atcatccgca ccgcgcggcg
    22921 cctggggatc ggcacggtcg cggtctactc ggaagccgac gccggcgcgc tgcacgtgcg
    22981 gatggccgac gaggcggtgg cgatcggacc ggcgccgagc agcgacagct acctcgccct
    23041 cgagcgcgtg gtcgacgcgg caaaacgggc gggtgcggac gcggttcatc ccggcttcgg
    23101 cttcctggcc gagaacgcgg ccttcgcgcg cgcgcttgcc gaggccggca tcgtgttcgt
    23161 cgggcccggg cccgcggcga tcgcggcgat gggcgacaag ctcgcctcca agaagctcgc
    23221 cgagcgcgcg ggggtgaaca tcattcccgg ccacgcgcac gccatcgccg acgtcgatga
    23281 ggcggcgcgc gtcgccgccg gcctcggcta tccggtgatg atcaaggcgt ccgccggcgg
    23341 cggcggcaag ggcatgcgcg ccgcccgcga cgaggccgag ctgcgcgacg gcttcgacgc
    23401 cacccggcgc gaggcgagga gcgcgttcgg cgacgaccgt atcctgatcg agcggttcat
    23461 cgccgagccg cgccacgtcg agatccaagt gctggcggat tcctacggca acgcgatcca
    23521 cctgaacgag cgcgaatgct cgatccagcg ccgccaccag aaggtcatcg aggaggcgcc
    23581 gagcccgctg gtcggcgccg agttgcgccg cgttatgggc gcgcaggcgg tggcgctggc
    23641 gcgcgcggtc gactacgtct cggcgggcac cgtcgagttc gtggtcggtc ccgacaggca
    23701 gttctacttc ctcgagatga acacccgcct gcaggtcgag cacccggtca ccgagatgac
    23761 cgccggcctc gatttggtcg agcagatgat ccggatcgcc gccggcgaaa ggcttgcgat
    23821 cacccaggac caggtgccgc tccaaggctg ggcgatcgag gcacgcgtct atgccgagga
    23881 cccctcgcgc ggcttcctgc cctcggtcgg ccggctgacc cggttcatcc cgcccgccga
    23941 gggcgccggc gtgcgcgtcg atgccggggt cgccgagggc gacgagatca cccggttcta
    24001 cgatccgatg attgccaagc tcatcgttca cgatgcgagc cgcgaggcgg cgatcgcgcg
    24061 gatgcgccgg gcgctcgacc gcttcgtggt acacggcgta cggaccaaca tcgcgttcct
    24121 ggccgcggtg cttgctcatc cgcgcttcgc cgacggccgg ctcagcaccg gcttcatcgc
    24181 cgaggtgttc ggcgcgggct acgacgccgc cgaggcgccg gcggccgtgg ccgatgtgct
    24241 cgccgcggtc gcggccttcg cagaccggcg caccgccgag cgcgccgccg ccatcggcgg
    24301 gttggttccg gcgcgcgagt gccggctcgc gatcgcgatc ggcgcgcagc ggatcgacgc
    24361 cgccgtcagt gcaatcgagg ccggctaccg ggtcgccctc gactcgcgaa tccttaccgt
    24421 ggcaagcgac tcctggcgcc ccggcctcga cctgttcgag ggccgcgtcg acggccgcga
    24481 ggtcgcggtc cagatccggc gcggcgcgcg cgggacgtgg cagctggtcc atggcggcgc
    24541 cgaggtcgag gtcctggtgc gcggcgcgcg cgccgccgag ctggcggcgc gcatgccggc
    24601 caagcaggcg gccgacctgt cgaagctgct gctgtcgccg atgcccgggc tgatggtctc
    24661 gctggcggtc gcggacggcg atcaggtcaa ggccggtgag cccttgtgcg tgatcgatgc
    24721 gatgaagatg gagaacgtgc tgcgtgccga acgcgacggc acggtcgccg agatcaaggt
    24781 ccgccccggc gacagcgtcg ccgtcgacca ggtgctgatg acgttcgagt gagcgcgctc
    24841 acccgcccgc gtcgggcgcg ggcatcatga accgcgcaag ctcggtctgg gtgcgcagga
    24901 tgccggcggc gcgctccgcg cgcttgacga ttcgctcggc cgcgaagcca agctggacca
    24961 tcgcgatgcc ggcatcgaag atctcgtcgt aggccgcgat cagaccggcg gccaggcgga
    25021 accggccgat tccctcgaac accacccggc ggcccttggc gccgttgaac ttcgcggtgt
    25081 aggcgaacac gtagtgggcg tagccgacat ggtcactcaa cgccgggtcg tacatcttcc
    25141 aggcgaagtc ctcggcgtcg cggcggaagt ggttgtcgat catgtcggcg atcgctgccc
    25201 ggccgtggaa cgcgccatag aagccgtcgt ggtagacacc gtcctcggcg aacaaggcgg
    25261 caaggtcggc gccggtgccg gcggtcgcgg cggcgcagaa ctgctcggtc aacgcaacga
    25321 aatccatctc cgatatcctc gctgccgtcg ggacgtggca tcatgcgcga atgaccggcg
    25381 agggggcaat ggctttgccc gtcgggcaca aggcggagcg ggtgcgcatc agcgggcggg
    25441 tccagggcgt ctggttccgc ggctggaccg tggacgaggc gcgccggcgc ggcctcgacg
    25501 gctgggtgcg caacctccgc gacggctcgg tcgaggcgct gttctcgggc cccgccgagg
    25561 cggtcgacga catggtcgcg gcgtgccggc gcgggccgcc ggcggcgcgg gtcgataacc
    25621 tggtgcggcg cgcggccgcg gccgtgcccg gcaccggctt caccgccaag ccgaccgtgt
    25681 aaccgcccgg ttacgccttc ggccgccgcc gtggccggct caccagctcg ccgccgcagt
    25741 tggggcaggt gaaggccatc gcgccggcgc agccggcaca gaaggtgcac tcgtacgagc
    25801 agatgaaggc gtcggcctgg tggccgagcg gcgcctcgca cgtctcgcag gccggcctca
    25861 tctcgagcgc catggcagtc tccctcagct tgccgcccgg gccggctcgg cgtcatcgac
    25921 cgtggcgccg aattggcgct cgaactgcag cctgaggcgc gcgtcgacct cggccatggt
    25981 cgcagcgacg ccaagggcgg cgagcgaggt gacgccgtag ccggcgagcc cgcacggcac
    26041 gatgccccgg aaattcgcaa gctccggcgc gacgttgagc gacacgccgt gataggtcac
    26101 ccagcggcgc acccgcacgc cgagcgcgcc gatcttcgcc tcgctgccgt ccgggcgcgc
    26161 cacccacacc ccgatccgcc cggcgcgccg cccggcgcgc acgccgaagt cggccagggt
    26221 atcgatcagc caggcctcga ggccgctgac gaacgcgcgc acatcggcgc gccgccccag
    26281 cgcttgcaga tcgagcatga cgtagccgac gcgctggccc gggccgtgat aggtgaaccg
    26341 gccgccgcgc ccggtgcggt ggacgggcag ccgcaagggc tccagcagct ctgaatcctt
    26401 ggcgctggcg ccgcgggtat agagcggcgg atgctccaac agccacaccc gttcggctgc
    26461 gcggccggcg cggatcgccg ccacctcggc ttccatttcc gcgaccgcgg ccggataggg
    26521 gaccggcgct tggctgagcg tccaacgcac gggcgccatg gcggcgaccc taacacgtgt
    26581 tgaactgggg cgggagattt gctatctgcc ccccgggtca acagggtcgg gtccacaggg
    26641 tcggggctcg tgccgcgtgt gccctgcggt cgtggcggaa ctggtagacg cgcagcgttg
    26701 aggtcgctgt gggggcaacc ccgtggaagt tcgagtcttc tcgaccgcac catccatatg
    26761 accaggggcg cgaattcgcg agtcgcctga cgacagagca gtgaaccgga ccaggtagcc
    26821 cgtggtgcga tcacgccggg ctcccacagg ccaggatggg aggttgcaga tgatcggcac
    26881 cccttggttc ggccgctcgc gaacgaccat ctccagtgca ccgccggcga gcgggggtcg
    26941 gccgtgaccg acgccccggt cgacgacgcc cgccgcccgg cggccgagga caacggcgat
    27001 ccggcgcgcc cggtcacgcc cgagttcgtg cgcgacatcg ccgaggcgct ggccgccggg
    27061 cgcaccgcgg acgcggtcga gcgcgcgcgc accctccact ccgccgacct cgccgacctg
    27121 gtcgaggcgc tcaagcccga cgagcgggtg gcgctgatcg gggcgctggg cccgcatctc
    27181 gatcccgagg ttctggtcga cctcgacgag catgtccgcg acctggtgct cgaatcgatc
    27241 gagcccgagg cgatcgccgc ccacgccgcc gagctcgaca ccgacgatgc gacccacctg
    27301 atcgaggacc tggatgcggc cgaccagcag aaggtgctgg aggcgctgcc ggcatccgag
    27361 cgcgagctga tcgagaacgc gctcgcctac cccgagaaca gcgccgggcg gctgatgcag
    27421 cgcgactacg tggcggtgcc gtcctactgg gatgtcggcc aggtgatcga ctggctgcgc
    27481 gagaccgacg agctgcccga cgagttctac gagatcttcg tcgtcgaccc gcgccaccgc
    27541 ccggtcggca ccgtgccgct caaccgcgcg atgcgcacca agcgaccggt atcggtggcc
    27601 gagatcatgg accccgagcc gcgcctgatc ccggtcgaca tggaccagga ggaggtcgcc
    27661 taccagttcg accagtacgg gctcgcctcg gcggcggtgg tcgcgcccga cggccggctg
    27721 ctcggccaga tcacggtcga cgacgtggtc gacgtgatcc aggaagaggc cgaggaggac
    27781 atccaccggc tcggcggcgt cgccgcccaa ggcgacctgt acctgagccc gctcgccacc
    27841 gtccgccggc gctggatatg gctgctggtc aacctgctga cggcagtgat tgcctcactc
    27901 accattgcgc tgttcacgga caccatccag cagatcgtcg cgctcgccat cctgatgccg
    27961 atcgtcgcct ccatgggcgg caatgccggc acccagaccc tgaccgtcgc ggtgcgcgcg
    28021 ctggcgacgc gcgagctgac cgcggccaat gccgcgcgca tgctgtggaa ggagtgcgtg
    28081 gtcggcggca ccaacggcat cctgttcgcg atcgtcaccg gcgtcgtcgc ggcgctgtgg
    28141 ttcgagagcc cgatcatcgg cgtggtcatc ggcctggcga tgatcgtcaa cttgctggtc
    28201 gccggcgccg ccggtatgct gatcccgctc ggcctccagc gcgccggggt cgatcccgcg
    28261 atcagcggcg gcatcgtgct gaccacggtc accgacgtgg tcggcttctt cgccttcctg
    28321 gggctggcca agctgatcat cctgtgatcg cgcgtgccgc cggccggccg tgaccaagct
    28381 cacagcgccg gcacgggttt cgaggtagca acgatccgag cgaagagcgc ccggatcgcg
    28441 gcccgttcag ggcgttcaaa tccgcttgac gacggcttgg caggctggcc gcccgcagcc
    28501 cgggtgccca tattccggtc ggcgggaaga cgcctgggaa gcgcccgcga tgcggtttcg
    28561 cggggtgccg gtcacaggag gtggccatgg ccgatattcg gacccgcacc gtccccggct
    28621 ttgaggacgt cgtgctctgc gacgacgcgg acgcgggcct caaggcggta atcgcgattc
    28681 acgacaccgc cctcgggccg gctctcggcg gggtgcggat gtggcgctac gaaaatgagc
    28741 gggcggcgct cgacgatgcg ctgaggctgg ctcgcggcat gacgtcgaag gcggcggtcg
    28801 cgggcttgcc tttgggcggc ggcaaggcgg tgatcctggg cgatccgcgg cgcgacaaga
    28861 gcgaggccct gttccgcgcc ttcggccgca tggtcgagcg cctgcgcggg cgctacatca
    28921 ccgccgagga cgtcggcacc tcggtcgagg acatcgccac ggtcgcccgc gagacccgcc
    28981 atgtggccgg cctcgccgac ggcagcggcg acccctcgcc ggtgaccgcg gcgggcacct
    29041 ttcacggcat ccgcgccgcg gcccgccacc gcttcggcag tgacgacgtt gccgggctca
    29101 gggtcgcggt ccagggcgtc ggccatgtcg gctaccacct gtgccggctg ctgcacggcg
    29161 ccggtgccga cctggtggtg agcgacgtct cggcgaccat ggtggcgcgc gcggcggtcg
    29221 agttcggcgc cgcgacggtc gcgcccgatg cgatctacga cgcagacgtc gacctgttcg
    29281 cgccgtgcgc gctgggcggc gcgatcaatc gcgacaccgt cccgcggctc aaggcggcga
    29341 tcgtcgccgg cgccgccaac aaccagctcg ccgagccgat ggacgggcgc gcgctgcacc
    29401 ggcgcggcat cctgtacgcg cccgattacg tgatcaacgc cggcgggctg atcaacgtgt
    29461 cctgggacgt ccttcaccgc ggcgagcctt acgaccgcgc cgaggtcctg gaccaggtgg
    29521 cgcgcatcga cccggtgctg acagcgatct tcgcgcgcgc cgcggccgag ggccggccgg
    29581 ccaacgaggt tgccgacgca ctcgcccgcg gccgcctgcg cccggccgcg gcagcctgac
    29641 caactcccga acgcgggccc tgaagacgca agcccgggcc gcgacgcgcg cgacccgggc
    29701 ctgactgcgg cgcggaacgc gacgctactt gagcatgtcg gtgaccgcgt cgatccccgc
    29761 ctgggcgcac atctcgtcct cgtcggcgct ggcgccgctg acgccgatgc cgccgatctg
    29821 ctggccgccg gcggtcatga tcggcaggcc gccggggaac gaggcgatgc ccggaatgtt
    29881 ggcgatgccc gggatccgcc ccggctcgcc gtccttgccg aaggcgagct cgccgaacac
    29941 gcgcgagggg aacgggaaca tggtcgagga gtggcccttc atctgcgcga tctggatcga
    30001 gccgaggaac gccccctcca tgcgcttgaa caccttcagg ttgccgccgt cgtcgaacac
    30061 cgcgacgttg atcggccgcc agcccgaggc ggcggccttg gcgacgcacg cgtcggccat
    30121 catgttcgcc atttcgaggc tgatcaccgg cttctggtcg agcgcctggg cgcccgcgcc
    30181 gaggccgaga acgaccaccg ccgcgccggc ggcggcaacg agcttggaca tggtgtgcat
    30241 gaactcctcc ctgtcggctg aggcgaaagc cgcccatccg gtgcagaggc tatccgcaag
    30301 ccgcgagcgg tgcaaacgac aaacccgtga ctggcgccgg ggccggccct aaagcaggcc
    30361 gaggtcgcgc agctcctcga ccatctccgc cggcatgcgc agctgggcgt cgtcgccgcc
    30421 gcgcgggcgc gcatcgggcg gcgcgtccgc gggctcgagg tagcgccagc cctgcatcgg
    30481 ccggcagcgc cggggcgcgg tctcgaccag gtcgggatcg agcacgatcg cgcagcgctt
    30541 gagcgccgcc gcgtcgtccg cgcgctcgag cgcgaggatg cgctggcgga cgcgcacgga
    30601 gcccttgatg atccagtaca gcgagccctt accggcgatc tcgcccgccc ggcgcgggaa
    30661 gttgcgcgtc cagtgcctga gcgccggcac ctcgccggcg gcggcctggc gcgcaagccg
    30721 gccgccctgg agggcgcgca gctgatcgac gctgcccgcg ccaacgcaca tcttcagcag
    30781 gtgcacggtc atagcccatc tgtagcgcga ttcgccgcgc gttcgaagcc ccggcgcgcc
    30841 gcgtcatggc acgcggcccg tcctccgcag cagcggcagc gccgctgccg ctgcggaggg
    30901 tggaagacgc ggagggcgcg gagaagggtt gaaccacgga gggcacagag gacacagagt
    30961 atgtccttct ctgtgaactc tgtggtctct gtggttgata ctttcctggt cctctccgcg
    31021 gcctccgcgc ctccgcgtga ccccaacggt gcgtgcggct tgcctggggc ggcggtggag
    31081 gtagactgcc ggcgacaacg ccgggaggag gcgagcgatg gccgacgacg caaccagacc
    31141 gcgtttcgcg ggcgtgatcc cgcccgttct caccccgttc cgggctgacc tgagccccgc
    31201 ctgcgcgcgc ttcaccgcgc atgcccgctg gctgctcgac gagggcgcca ccgggctcgc
    31261 gccgttcggc accaccggcg aggccaactc tctctcggtc gacgagcggc tggtgctgct
    31321 ggagtcgctc ctcggcgccg gcatcgatcc ggccgtggtg atgccgggca ccggctgctg
    31381 tgcgctcacc gacagcgtgc gcctcaccga ggccgcggtg cgcgcgggct gcggcggggt
    31441 gctgatgctt ccccccttct actacaaggg ggtgagcgag gacggcgtgt tcgcgtccat
    31501 cgccgaggtg gtcgagcggg tcggcgacac gcgcctgcac gtctacctct accacatccc
    31561 cccggtcgcc caggtcggct tctcgctcag ccttatcgag cggctgatcg gcgcctaccc
    31621 cgagaccgtg gtcgggctca aggacagctc gggcgactgg gccaacacca aggcggtcat
    31681 cgacgccttc ccgggcttcg ccaccttctc gggctccgag gtgttcctgc tcgacaattt
    31741 gcgcgcgggc ggcgccggct gcatcaccgc gaccggcaac gtcaatgccg ccgcgatccg
    31801 cgcgctctac gaccgctggc agggggcgga cgcggattcg ctccaggccg acattacggc
    31861 gttgcgcaag accatccagg cgttcccgat gatcccggcg ctgaaggcga tcctcgcccg
    31921 gcacctcggc gatgccgact ggtcccgcac ccgcccgccg ctccgcgacc tcaccgccga
    31981 gcaggcccgc gcgctcaccg aagcgctgcg cacccgcgcc ttcgcgctcg cggcgtgacg
    32041 gctcaagtcg acaagatgtt tcaaagtcaa actgattccg gtgcctcgac aaagtcccgt
    32101 cattcccgcg aaagcgggaa tccatcgtcg ggccggaact cccgccgtcg cggctgtgaa
    32161 caagacgttg gggccgctcc gaattcgccg cctccgcgca cgcgggggtc cagttacccg
    32221 ttgagacgtc tggattcccg ctttcgcggg aatgacgaga atgggctttg gggggagaac
    32281 gacagccac
//
LOCUS       DASWHU010000146         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1547490, whole genome shotgun sequence.
ACCESSION   DASWHU010000146 DASWHU010000000
VERSION     DASWHU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547490"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGB88_05650"
     CDS             <1..243
                     /locus_tag="VGB88_05650"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9646960.1"
                     /translation="RRRLERGVKLNHPEAVALITDHIVEGARDGKSVAELMSEGAAVL
                     RRVQVMEGVAEMIGEVQVEATFPDGTKLVTVHAPIR"
     gene            255..575
                     /locus_tag="VGB88_05655"
     CDS             255..575
                     /locus_tag="VGB88_05655"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX9646961.1"
                     /translation="MIPGEILPAAGEIVLNADREAITLTVTNTGDRPVQVGSHYHFFE
                     TNPALAFDRDRARGLRLDIPAGTAVRFEPGQARDVHLIPYAGRRRAVGFNRRVMGPLD
                     GAPG"
     gene            579..>1819
                     /gene="ureC"
                     /locus_tag="VGB88_05660"
     CDS             579..>1819
                     /gene="ureC"
                     /locus_tag="VGB88_05660"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX9646962.1"
                     /translation="MATPIGRRAYADMFGPTTGDKVRLADTELVIEVEDDRTTYGEEV
                     KFGGGKVIRDGMGQSQATRADGAVDTVITNALILDHWGIVKADIGLRDGRIAAIGKAG
                     NPDTQAGVDIVIGPGTEAIAAEGLIVTAGGIDAHIHFVCPQLVEAALASGVTTMLGGG
                     TGPAAGTNATTCTPGPWHIARMLQAAEAFPVNLGFFGKGNASAPAALVEQVEAGACGL
                     KLHEDWGTTPAAIDCCLAVADDHDVQVLIHTDTLNESGFVENTIGAFKGRTIHAFHTE
                     GAGGGHAPDIIRLCGEANVLPSSTNPTRPFTVNTVDEHLDMLMVCHHLDPRIPEDVAF
                     AESRIRKETIAAEDILHDLGAFSMIASDSQAMGRVGEVIIRTWQTADKMRRQRGRLPE
                     ESGDNDNFRAKRYIAKYTINPA"
BASE COUNT          303 a          683 c          606 g          227 t
ORIGIN      
        1 cgccggcggc tcgagcgcgg ggtcaagctc aaccaccccg aggcggtggc gctgatcacc
       61 gaccacatcg tcgagggcgc ccgcgacggc aagtcggtcg ccgagctgat gagcgagggt
      121 gccgcggtgc tgaggcgcgt ccaggtgatg gagggcgtgg ccgagatgat cggcgaggtc
      181 caggtcgagg cgaccttccc cgacggcacc aagctggtca ccgtccacgc gccgattcgc
      241 tgacggaggc gcccgtgatt cccggagaga tcctgcccgc cgccggcgag atcgtgctga
      301 acgcggaccg cgaggcgatc accctcacgg tcaccaacac cggcgaccgg ccggtgcagg
      361 tgggcagcca ctaccacttc ttcgagacca acccggcgct cgccttcgac cgcgaccggg
      421 cgcggggcct gcgcctcgac atcccggccg gcaccgcggt ccgcttcgag cccggccagg
      481 cgcgcgacgt tcacctgatc ccctatgccg gccgccgccg cgcggtcggc ttcaaccggc
      541 gggtcatggg cccgctcgac ggcgcgccgg gctgagccat ggccacgccg atcgggcgcc
      601 gcgcctatgc cgacatgttc ggcccgacca ccggcgacaa ggtacgcctc gccgacaccg
      661 agctggtcat cgaggtcgag gacgaccgca ccacctacgg cgaggaggtc aagttcggcg
      721 gcggcaaggt gatccgcgac ggcatgggcc agagccaggc gacgcgcgcc gacggcgcgg
      781 tcgataccgt catcaccaac gcgctgatcc tcgaccattg gggcatcgtc aaggccgata
      841 tcggcctccg cgacggccgc atcgccgcga tcggcaaggc cggcaacccg gacacccagg
      901 ccggcgtcga catcgtcatc ggccccggca ccgaggcgat cgccgccgag ggcctaatcg
      961 tcaccgccgg cggcatcgac gcccacatcc atttcgtctg cccgcagctc gtggaggcgg
     1021 cgctggcgag cggggtcacc accatgctcg gcggcggcac cgggcccgcc gccggcacca
     1081 acgccaccac ctgcaccccc gggccgtggc acatcgcgcg catgctgcag gcggcggagg
     1141 cgttccccgt caacctcggc ttcttcggca agggcaacgc cagcgcgccg gcggcgctcg
     1201 tcgagcaggt cgaggccggc gcctgcggcc tgaaattgca cgaggactgg ggcaccacgc
     1261 ccgcggcgat cgactgctgc ctcgcggtcg ccgacgatca cgacgtccag gtgctgatcc
     1321 acaccgacac cttgaacgag tcggggttcg tcgagaacac catcggcgcg ttcaagggcc
     1381 gcaccatcca cgccttccac accgagggcg ccggcggcgg ccacgcgccc gacatcatcc
     1441 ggctgtgcgg cgaggccaac gtgctgccgt cgtcgaccaa cccgacccgc ccgttcacgg
     1501 tcaacacggt cgacgagcat ctcgacatgc tgatggtctg ccatcacctc gatccgcgca
     1561 tccccgagga cgtggcgttc gccgaaagcc gcatccgcaa ggagacgatc gcggccgagg
     1621 acatcctcca cgacctcggc gcattctcga tgatcgcctc cgacagccag gcgatgggcc
     1681 gggtcggcga ggtgatcatc cgcacctggc agaccgccga caagatgcgc cgccagcgcg
     1741 gacgcctgcc cgaggagtcg ggcgacaacg acaatttcag ggctaagcgt tatatcgcca
     1801 agtacaccat caacccggc
//
LOCUS       DASWHU010000147         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3382409, whole genome shotgun sequence.
ACCESSION   DASWHU010000147 DASWHU010000000
VERSION     DASWHU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3382409"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGB88_05665"
     CDS             <1..484
                     /locus_tag="VGB88_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX9646963.1"
                     /translation="EESARGGKRRVTLGGGRSLDVAIPEGIEDGQSIRLKGQGEPGIG
                     GGPAGDALIRVQVEPHPVFTRDGSNLRMELPVTLVEAVVGAKVEVPTLDGPVTMTVPA
                     GSNTGDTLRLRGKGIRRRGASGRGDQYVRLKVVLPRKPDDELGTFVRDWAQRHPYKVR
                     "
     gene            617..1990
                     /locus_tag="VGB88_05670"
     CDS             617..1990
                     /locus_tag="VGB88_05670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9646964.1"
                     /translation="MSVLAAPALADDAPAPAEDRDTLSPAQLRIEERLRRVEAVTELF
                     NRQTRLWRLSYPLLYGAVDMCPDRIRASAGFSYANAYLFRGAARSAANALGYDHRVSV
                     VDVAPGSAAAAAGIQRRDVLVAIGDWQSPIGDGAVSLAAAMLRDTLERSGAAVLTMAR
                     GHDSYQAKIKPDYICDLQVIAAPGDMLSAYADGDRVVVFHGMMDFIADDGELAAVIAH
                     EIANSLLPGLGIAGSPSDYSLSNFFNWGMEPEATGDPVDDRARAHDRGLAADYVAAYL
                     LARAGFDHRALIGLWRRVAAAGLVDADGGYSIPDAERTARLAATIVEVDAKLASHGAL
                     APSPDGPPAAPAEASAATVEVAAPAATVEFVDLPWAAPVETVAVETEVPDIPADAPIP
                     AAGIEATGAVEVEGMPWTAPARLLPTDESMHQDVAMTALGAPEPAADVQPALDRAGFS
                     FVDWFGR"
     gene            2108..2944
                     /gene="fabI"
                     /locus_tag="VGB88_05675"
     CDS             2108..2944
                     /gene="fabI"
                     /locus_tag="VGB88_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX9646965.1"
                     /translation="MSDLTAQIAHAPAPLLAGRRGVVMGVANDRSLAWGIARTAAAHG
                     ADIAFTYQGEALAKRVHPLAASVGSTMVLACDVADEASIDAAFARVLERFGGIDFVVH
                     AIAYSDKEELKGRYLDTSRDNFARTLDISCYSFTAVCRRAALTMKPGGSLLTLTYSGA
                     ERVMPHYNVMGVAKAALEASVRYLAVDLGGAGIRVNAISAGPIKTLAASGIGDFRYIL
                     KWNEYNSPLKRNVTIDDVGGAALFLLSDLSSGVTGEVVHVDAGYHVVGMKAVDAPDIA
                     VV"
     gene            2958..4037
                     /gene="aroC"
                     /locus_tag="VGB88_05680"
     CDS             2958..4037
                     /gene="aroC"
                     /locus_tag="VGB88_05680"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9646966.1"
                     /translation="MAGNSFGALFRFTTWGESHGPEIGCVIDGVPPRIPLEESDIQPW
                     LDRRRPGQSRHTTQRREADRVRIVAGTFEGVTTGTPLALAIENVDARPKDYSDIKDKF
                     RPMHADYTYWRKYGVRDYRGGGRASARETAMRVAAGAVARKVLGESVRVRGAMIRMGR
                     HAIDRANWDWDEVARNPFWCPDAAVATAWEDELDALRRAGNSVGAEIEIVAEGVPAGW
                     GDPVYDKLDADLAKAMMSINAVKGVEIGAGFAAASMTGLDGADEMRAGRGVEAPEFLS
                     NYAGGILGGISTGQDIVVRFAVKPTSSIPAPRRTIDTEGRETEISTTGRHDPCVGIRA
                     VPVGEAMMACVLADHMLRHRAQTGG"
     gene            4628..>4965
                     /locus_tag="VGB88_05685"
     CDS             4628..>4965
                     /locus_tag="VGB88_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007118683.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX9646967.1"
                     /translation="MARADRLWALGIEPDLDALDDRMKHYFEVCRDKLGLLPNVLRAH
                     AFKPARLANFVNTYNELMLGDSPLSTLEREMIAVAVSSVNKCFYCLVAHGAAVRALSG
                     DPVLGEAIAH"
BASE COUNT          741 a         1747 c         1776 g          701 t
ORIGIN      
        1 cgaggaatcg gcgcgcggcg gcaagcggcg cgtcaccctg ggcggcgggc gcagcctcga
       61 tgtcgccatc ccggagggga tcgaggacgg ccagtcgatc cggctcaagg gccagggcga
      121 gcccggcatc ggcggcgggc cagcgggcga tgcgttgatc cgggtccagg tcgagccgca
      181 cccggtgttt acccgcgatg gtagtaattt gcgcatggag ctgccggtca ccctggtcga
      241 ggcggtggtc ggcgccaagg tcgaggtgcc caccctcgac gggccggtca ccatgaccgt
      301 gcccgccggc tcgaacacgg gcgacaccct gcgcctgcgc ggcaagggca ttcgccgacg
      361 cggcgcctcg ggccgcggcg accagtacgt ccggctcaag gtggtgctgc cgcgcaagcc
      421 cgacgacgag ctcggtacgt tcgtccgcga ctgggcgcag cgccacccct acaaggtgcg
      481 ctgagccggc ggcgggcttc cccggcttgt cacgcgagcg tgccgatact agtactggcg
      541 cggccggccg gtgggcggag gcatgaaggg gagcgcgatg cggcgagccc gatggggcgg
      601 actccttggc ctggcgatga gcgtcctggc ggcgccggcg ctggccgacg atgcgccggc
      661 gccggccgag gaccgcgata ccctgtctcc cgcccagctg cggatcgagg agcggctgcg
      721 ccgggtcgag gcggtcaccg agctgttcaa ccgccagacc cggctgtggc ggctgagcta
      781 cccgctgctc tacggcgcgg tcgacatgtg tcccgaccgc atccgcgcca gcgccgggtt
      841 ctcctacgcc aacgcctacc tgttccgcgg cgccgcgcgc agcgccgcca acgcgctggg
      901 ctacgaccac cgggtctcgg tggtcgacgt ggcgccgggc agcgccgccg ccgccgccgg
      961 catccagcgc cgcgacgtgc tggtcgcgat cggcgactgg cagtcgccga tcggcgacgg
     1021 cgcggtgtcg ctggccgcgg cgatgctgcg cgacacgctc gagcgctccg gcgccgcggt
     1081 gctgacgatg gcgcgcggcc acgacagcta ccaggccaag atcaagcccg actacatctg
     1141 cgacctgcag gtgatcgcgg cgcccggcga catgctgagc gcctatgccg acggcgaccg
     1201 ggtggtggtg ttccacggca tgatggactt catcgccgac gacggcgagc tcgccgcggt
     1261 gatcgcgcac gagatcgcca acagcctgtt gcccggcctc ggcatcgccg gcagcccgag
     1321 cgactactcg ctgtccaatt tcttcaactg gggcatggag cccgaggcga ccggcgatcc
     1381 ggtcgacgat cgcgcgcggg cgcacgaccg cggcctcgcc gccgactatg tcgccgctta
     1441 tctgctcgcc cgcgccgggt tcgaccaccg cgccctgatc gggctgtggc ggcgggtcgc
     1501 cgcggcgggc ctggtcgatg ccgacggcgg ctattcgatt cccgacgccg agcggaccgc
     1561 ccggctggcg gcgaccatcg tcgaggtcga tgccaagctc gcaagccatg gcgcgctcgc
     1621 ccccagcccc gacggcccgc ccgcggcacc ggcggaagcg tccgcggcga cggtcgaggt
     1681 ggcggcgcct gcggcgacgg tcgagttcgt ggacctgccc tgggcggcgc cggtcgagac
     1741 cgtggcggtc gagaccgagg tgccggacat tcccgcagac gcgccgatac cggcggccgg
     1801 catcgaggcc acgggcgcgg tcgaggtcga gggcatgccg tggacggcgc cggcacggct
     1861 gctgccgacc gacgagtcga tgcaccagga cgtggcgatg acggcgctgg gcgcgcccga
     1921 gccggctgcc gacgtgcagc ccgcgctcga tcgcgccggc ttttcgttcg tcgactggtt
     1981 cgggcggtga cattttccgg caccgggcgg gcgcggcttt cccgccgtcc gggcatggtg
     2041 taggttggcg ccgctcggcc ccgatcccgc tcgcgaagcc cccgtgtccg gtcaattcga
     2101 catcatcatg tccgatctta ccgcccagat cgcccacgcg cccgcgccgc tgctcgccgg
     2161 ccgcaggggc gtcgtgatgg gtgtggccaa cgaccgctcg ctcgcctggg gcatcgcccg
     2221 gaccgcggcg gcgcatggcg ccgacatcgc cttcacctat cagggcgagg cactggccaa
     2281 gcgcgtccac ccgctcgccg cctcggtcgg ctcgaccatg gtgctggcgt gcgacgtcgc
     2341 cgacgaggcg agcatcgatg ccgcgttcgc gcgcgtgctc gagcgcttcg gcggcatcga
     2401 cttcgtggtc cacgccatcg cctactcgga caaggaggag ctcaagggcc gctacctcga
     2461 caccagccgc gacaacttcg cgcgcacgct cgacatctcg tgctactcgt tcaccgccgt
     2521 ctgccggcgc gcggccctga ccatgaagcc gggcgggtcg ctgctcacgc ttacctattc
     2581 gggcgccgag cgggttatgc cgcactacaa cgtgatgggc gtggccaagg cggcgctgga
     2641 ggcgagcgtg cgctatttgg cggtcgacct gggcggcgcc ggcatccggg tgaatgcgat
     2701 ctcggccggg ccgatcaaga ccttggcggc gagcggcatc ggcgacttcc gctatatcct
     2761 caagtggaac gagtacaact cgccgctcaa gcgcaacgtc accatcgacg acgtcggcgg
     2821 cgcggcgctg ttcctgctca gcgaccttag cagcggggtg accggcgagg tggtccatgt
     2881 cgatgccggc taccacgtgg tcgggatgaa ggcggtcgac gcgcccgaca tcgcggtggt
     2941 ctgaccgccc cgcagccatg gccggcaaca gcttcggcgc cctgttccgc ttcaccacct
     3001 ggggcgagag ccacgggccc gagatcggct gcgtgatcga cggcgtgccg ccgcgcattc
     3061 cgctcgagga gtcggacatc cagccctggc tcgaccggcg ccgacccgga cagtcgcgcc
     3121 acaccaccca gcgccgcgag gccgaccggg tgcgcatcgt cgccggcacc ttcgagggcg
     3181 tgaccaccgg cacgccgctc gcgctcgcga tcgagaacgt cgatgcccgg cccaaggact
     3241 actccgacat caaggacaag ttccgcccga tgcacgccga ctacacgtac tggcgcaagt
     3301 acggcgtgcg cgactatcgc ggcggcgggc gggcgagcgc gcgcgagacc gcgatgcggg
     3361 tcgccgccgg cgcggtcgcg cgcaaggtgc tgggcgagag cgtgcgcgtg cgcggggcga
     3421 tgatccggat gggccgccac gccatcgacc gcgccaactg ggactgggac gaggtcgcgc
     3481 gcaacccgtt ctggtgcccc gatgcggcgg tcgcgacggc gtgggaggac gagctcgacg
     3541 cgctcaggcg cgcgggcaac tcggtcggcg ccgagatcga gatcgtcgcc gagggcgtgc
     3601 cggccggctg gggcgatccg gtctacgaca agctcgacgc cgacctcgcc aaggcgatga
     3661 tgtcgatcaa cgctgtcaag ggggtggaga tcggcgcggg ctttgccgcc gcctcgatga
     3721 ccgggctcga cggcgccgac gagatgcgcg ccggccgcgg cgtcgaggcg cccgagttcc
     3781 tgtcgaatta cgccggcggc atattgggcg gcatctcgac cggccaggac atcgtggtcc
     3841 gcttcgcggt caagccgacc agctcgatcc ccgcgcccag gcggaccatc gacactgagg
     3901 gccgcgagac cgagatctcg accaccggcc gccacgatcc ctgcgtcggc atccgcgcgg
     3961 taccggtggg cgaggcgatg atggcctgcg tgctcgccga ccacatgctc cgccaccgcg
     4021 cccagaccgg cggctgatgc ccaatgccta tccgaattga gtcgtcattc cgggcgcacc
     4081 gcagcgcgaa gcggtgcggt gcagacccgg aatccattag tgccgcaacg agacccgggc
     4141 tcagtgacac atcaccgcgt gccgcatcgg ttccgggacg acggagtgcg gagaatggtc
     4201 gggcgtcgtt cgcctcacgc ggaggcgcgg aggacgcgga gcagaaagaa aggcaaacca
     4261 cagagggcac agaggccaca gaggtaaggt ttctctgtgt ccgctgtggt taattccggc
     4321 caagccatct ccgcgatctc cgcgcctcgg cgtgcttcat ggctgctcac cgccggaaat
     4381 gctcgcgaaa tgcgattgct ttgcccgctc gcgggggagg gcgccatttt catggcctcc
     4441 ccccgctggc ggaaggcagg tgggagtgga tggattgacc gttatcggaa tccgctctga
     4501 agcaagctca atcgttagag aggcaactat ggcgaagtcc aagaccagcg gcaagaccgc
     4561 gcccaagcgc actgcggcaa aaaagaagtc cgacaaaaca gcgcgcgcgc cgcgcacgag
     4621 tcgcggcatg gcgcgggcgg accggctgtg ggccctcggc atcgagcccg acctggacgc
     4681 gctcgacgac cgcatgaagc actatttcga ggtctgtcgc gacaagctcg ggctgctgcc
     4741 caacgtgctc agggcgcatg cgttcaagcc cgcgaggctc gccaacttcg tcaacaccta
     4801 caacgagctg atgctgggcg actcgccctt gagcacgctc gagcgcgaga tgatcgcggt
     4861 cgcggtctcg agcgtgaaca agtgcttcta ctgcctggtc gcccacggcg ccgcggtgcg
     4921 cgcactctcg ggcgatcccg tcctcggcga ggcgatcgcg cacaa
//
LOCUS       DASWHU010000148         9749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1926484, whole genome shotgun sequence.
ACCESSION   DASWHU010000148 DASWHU010000000
VERSION     DASWHU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926484"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1059
                     /locus_tag="VGB88_05690"
     CDS             25..1059
                     /locus_tag="VGB88_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9646968.1"
                     /translation="MHAITIGLDIAKSVFQVHGEDAQGRIVLQKRLRRSQVVPVFAKL
                     PPALVGIEACGSAHHWARQLQALGHEVRLIPAAYVKPFVQRNKTDARDAAGICAALGR
                     ADMRFVPVKSVGQQASRGLERSRELLVKQHTQLMNCTRSLLAELGIVAAQGRQGFAAL
                     AALVVEGDERIPAVLRLALSHLLRQIEQLRRSIAALEEEIMAVAKADPVMRRLATIPG
                     VGGLTAYAIVAAVGDGRRFASARDFAAWCGLTPRQHSSAGKRRDKGISRQGDIRLRKL
                     FALGASTVMRNARAQPARASEWQRGILARRPVKVAVLAQAAKTARIAWAVLTSGESYR
                     APGAAVAAAA"
     gene            complement(1533..2228)
                     /locus_tag="VGB88_05695"
     CDS             complement(1533..2228)
                     /locus_tag="VGB88_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646969.1"
                     /translation="MTLAGKGVMAFWNDIAPGAEDDFNHWHVAEHIPERVGVPGFLRG
                     RRYVAHDASPKYFNFYETESLATLTSPAYLARLNDPTPWTKRAIQTFRNSNRTLCRVI
                     ASLGRGEGGAMLTVRFAPAADAAARVRDRLAGELLATLVERPGVVGAHLLRGDAEASR
                     AETEEKALRAAPDQIADWVLLVDAIEPETLAGLRAGDLADRVLSGHGLADIAAGIYRL
                     HYSLRQEDLAGAG"
     gene            complement(2256..2750)
                     /locus_tag="VGB88_05700"
     CDS             complement(2256..2750)
                     /locus_tag="VGB88_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9646970.1"
                     /translation="MDSFLTEVQYWHWWIAGVALVALEMLVPGTFFLWLGVSAGITGL
                     VLLVLPALDWQYQILVYSVLSVGSIYVGRKVWRPGAEPTDEPALNRRGEQYVGRIVRL
                     AEPIAGGRGRARIGDSLWLVAGEDMPVGTEMRVVGVKGTVLEVARLDASKAKAGTEAD
                     TGHA"
     gene            complement(2763..3716)
                     /locus_tag="VGB88_05705"
     CDS             complement(2763..3716)
                     /locus_tag="VGB88_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9646971.1"
                     /translation="MQPIGLTIFVVVVLVLAVILVFLSVKSVPQGREFTVERFGRYTR
                     TLRPGLNLIVPVIDGVGAKLNMMEQVIDVPSQEIITKDNAMVRVDGVLFFQVLEAAKA
                     AYEVADLELAIVNLTVTNLRTVMGSMDLDELLSQREVINARILHVVDEATNPWGVKIT
                     RIEVKDISPPADLVESMGRQMKAERDRRAVILVAEGDRQAQILRAEGSKQAAVLEAEG
                     RREAAFRDAEARERSAEAEARATTMVSEAISKGNVQAINYFVAQKYVEALGNIASASS
                     SKIIMMPLEAANVIGAVAGIAELAKEAIDRRGNSGAAQTSP"
     gene            complement(3841..4200)
                     /locus_tag="VGB88_05710"
     CDS             complement(3841..4200)
                     /locus_tag="VGB88_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646972.1"
                     /translation="MKPGPAAVKCAVASAAAIFAVLVALAPAARAEECTAVHEVAITY
                     TFEPGDIYVAPGDCIHFTNIHGIEHSAVGLEREFNTGILMPGGTALIEFDEETVIPYV
                     CGVHPLMSGVIIVKSPD"
     gene            complement(4317..4913)
                     /locus_tag="VGB88_05715"
     CDS             complement(4317..4913)
                     /locus_tag="VGB88_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9646973.1"
                     /translation="MKARAVLAAVVATAVLWPACGALAEDAPAASPRAFALVDHLGRQ
                     VTDRDFLGSFVLLFFGYSHCPDICPTELAVIADAIERLGAPGTRVQPLFITIDPARDT
                     AEALAAYVGSFHPRLIGLTGSPAQIAAVAAGYRVGVHKVEPEGDGGPGADDYVIDHTA
                     ASYLIGPDGRVLGLFQHGTPAADIAAAIRPLLAGTRAD"
     gene            5079..7211
                     /locus_tag="VGB88_05720"
     CDS             5079..7211
                     /locus_tag="VGB88_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9646974.1"
                     /translation="MRHLRRGRVALAVAVAAAAVGAPARAGMDEPLATRLAPELVAEM
                     FPDAESPPAVSGDPPIATVRRAGELAGYLFSTHEMVHPAGYAGNSFDIVVGLDAHGVI
                     LGNRLLEEHEPLIGPGMITPSSFRRFLGELTGVDIKTTRRFVPKGVDGVSGATVSAII
                     MGDAILDSALRAGHLTGLIGAGDEGLAIDRFSFEPESWAGLVGDGSIASLSLTNAEVR
                     AAFAEAFGADAAPELGADQAPFIALHVALATPPRIGRNLFGARAFRQIAQDARPGEQQ
                     LLIASTGPYHWLPANPYLVAAFDRLHIVQRGTVLEIRPENFNPARRLAVEDQPRFTNA
                     GRLSIAPEAGFDPLAPWTLELLVDERAQDGAAPRALAFALPYLLPARYVLGDERALED
                     AGYRTPRYVGFGLFRDSTLNGWQRVWADKAGAIAGLVALLAAVTAVMLVQHRLTRHRR
                     LYALVRTGLLAATLVWLGWIAGAQLTVLTVLSYLRLPFDAGGWRSLLFDPLLVILTGY
                     VAVSLVLWGRGVFCGWLCPFGALQELLNKAARLLGAPQLQVGEPVQRRLWAIKYVVAI
                     GIVALAAYSLSTATVAAEVEPFKTAITLGFRRSWPFVAYAGLLLAAGLSIERFFCRYL
                     CPLGALLAIGGRLHLFEWLARRAECGSPCQICRQSCPVGAIRRSGAIDMDECLQCLDC
                     QVDYHDPHRCPPLVALRKHAVEAAVAAA"
     gene            7400..8722
                     /locus_tag="VGB88_05725"
     CDS             7400..8722
                     /locus_tag="VGB88_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9646975.1"
                     /translation="MEFNFLNYFKDFGDERVSLTDPYQPGTAFFRSGGLGKKVRARMD
                     DMADKSELPRREFFKSALGFTGAMLAVNAATGMRFFEVSEAEAREFAALEEVGESIKG
                     KMGAVIDMHTHVCTRPEGYVEGVNTTKQGMWFVRLLDDLGKAFGLENGTADMNVENYG
                     KMLLEGSDTDIGVFNPFGFREDYGGKDMVPIEYQAQVKKNWPSQTIMLAGGLTPNQGV
                     SETLDRLEMYVKDYKVSGLKLYTFDSTKAKGWWFDDEERAYPIWEKCRELGIKNIGCH
                     KGIPFGQFMARYAHAEDFDKACDDFLDLNFIAFHSAWPYHGELAALKGFKPQRNNMFS
                     EVGSTFAATVTSRPVECAHVIGTLVRDLGADYVMWGTDSLLWGNPQWQIDAFRRFRIP
                     DELIDGYGYPQLTDEVKTKIFGGNAARLWGLKTAMNDKGRQVPVVDFG"
     gene            8806..9105
                     /locus_tag="VGB88_05730"
     CDS             8806..9105
                     /locus_tag="VGB88_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX9646976.1"
                     /translation="MPTYEYKCEPCRTIYKVHHGMNETPVIRCPECNTKTERMISAPN
                     LSLGGYSSPTEAKYAKMSERDEVARENELQKTYKTIWLPEEVKHAPPWADDDHHH"
     gene            complement(9378..>9749)
                     /locus_tag="VGB88_05735"
     CDS             complement(9378..>9749)
                     /locus_tag="VGB88_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9646977.1"
                     /translation="VDFAGAARTPIPCRPGALRRALRNLIDNAATYGRRARVSLDGDA
                     AELWVVIDDDGPGIAPADRERAFAPFVRLEPSRSRDTGGIGLGLAIARTIVRNHGGDI
                     TLENRAEGGLRATVHLPRAGT"
BASE COUNT         1456 a         3399 c         3406 g         1488 t
ORIGIN      
        1 ccaccagcag aggagaccgt ccagatgcac gctatcacga tcgggttgga tatcgcgaag
       61 tcggtgtttc aggtccacgg cgaggatgcg cagggcagga ttgttctgca gaagcggctc
      121 aggcgctcgc aggtagtgcc cgtcttcgcc aagctgccac cggcgctggt gggcatcgag
      181 gcctgcggct cggcgcatca ttgggcgcgg caattgcagg cgctgggcca tgaggtgcgg
      241 ctgatcccgg cggcctatgt gaagcccttc gtccagcgca acaagaccga tgcgcgcgac
      301 gcggcgggca tctgcgcggc actggggcgg gcggacatgc gcttcgtgcc ggtgaagagc
      361 gtgggccagc aggcctcgcg cggactggag cgctcgcgcg agctgctggt caagcagcat
      421 acccagctca tgaactgcac gcgcagcctg ctggccgagc tcggcatcgt cgcggcgcag
      481 ggccggcagg gcttcgccgc gctggcggcg ttggtggtcg agggcgatga gcggataccc
      541 gcggtgctgc gcctcgccct ctcccacctg ctgcggcaga tcgagcagct gcggcgctcg
      601 atcgcggcgc tggaggagga gatcatggcc gtggccaaag ccgacccggt gatgcgtcgg
      661 ctggccacca tccccggcgt cggtggcctg accgcgtacg ccatcgtcgc cgcggtcggc
      721 gacggccggc ggttcgcctc ggcgcgcgac ttcgccgcct ggtgcgggct cacgccccgg
      781 cagcattcca gcgccggcaa gcgccgcgac aagggcatct cgcgccaggg cgacatccgc
      841 ttgcgcaagc tgttcgccct cggcgccagc accgtcatgc gcaacgcccg cgcccaaccc
      901 gcgcgggcca gcgagtggca gcgcggcatc ctggcccgcc ggccggtcaa ggtcgcggta
      961 ctggcgcagg ccgccaagac cgcgcgcatc gcctgggccg tgctcacctc cggcgagagc
     1021 tatcgcgctc ccggcgcggc ggtcgccgcc gccgcctaac tcgacaattg cttcccggtc
     1081 ggcgacggcg ccaggccggc gggtcagcaa ggagcagaag gcagcgacgg caaaatggtc
     1141 aggacctgga acgggacact ccgatacgtc atcgcgcttc caagcgcgcc gcaatgctgg
     1201 gagcccgttc cgcgataccc atcggggccg gcggtcgaca ggccgcagca acaggccgaa
     1261 tacatgaccg ctccagactc tttgccaaaa ccgcttctcg ccccttgcca gaggggggcc
     1321 gtccatacat gacgacgaga acagtggcct gagtccttat caatgcgatc cggtatgaga
     1381 tgtcctgagc gccggcgcgc cgcaccagca gggccgcgat cacggcaatg ccggcggcgg
     1441 cgaactcgac catgggcatt ggagaacggg ttggcgctgg ggtccgcctg ccggtgtcgc
     1501 agtcggcggc gcgccgtcaa cccggccgct cgctagccgg cgccggcgag gtcctcctgg
     1561 cgcaggctgt agtgcaggcg gtagatgccg gcggcgatgt cggcgaggcc gtggccggac
     1621 agcacgcggt cggcgagatc gccggcgcgc aagccggcga gcgtctccgg ctcgatcgca
     1681 tcgaccagca gcacccagtc ggcgatctgg tcgggcgcgg cgcgcagcgc cttctcctcg
     1741 gtctcggcgc ggcttgcctc ggcatcgccc cgcaacaggt gcgccccgac cacgcccggc
     1801 cgctcgacca gcgtcgccag cagctcgccc gcaagccggt cgcgcacccg cgccgccgca
     1861 tcggctgccg gcgcgaagcg cacggtcagc atcgcgccgc cctcgccgcg gccgaggctc
     1921 gcgatgaccc ggcagagcgt gcggttgctg ttgcggaagg tctggatcgc gcgcttggtc
     1981 cacggcgtcg ggtcgttgag ccgcgcgaga taggccggcg aggtcaaggt cgccaggctc
     2041 tcggtctcgt agaagttgaa gtatttgggc gatgcgtcgt gcgcgacgta gcggcgcccg
     2101 cgcaggaagc ccggcacccc gacccgctcg gggatgtgct cggccacgtg ccagtggttg
     2161 aagtcgtcct cggccccggg ggcgatgtcg ttccagaacg ccatcacgcc cttgcccgca
     2221 agcgtcatcg ccggccctcc cggtttccgg cgcgctcagg cgtggccggt gtcggcctcg
     2281 gtgccggcct tcgccttgga ggcatcgagg cgcgcgacct cgagcaccgt gcccttgacc
     2341 ccgaccacgc gcatctcggt gccgaccggc atgtcctcgc cggcgaccag ccacagcgaa
     2401 tcgccgatcc gggcacgccc gcggccgccc gcgatcggct cggccaggcg cacgatgcgc
     2461 ccgacatact gctcgccgcg gcggttgagc gccggctcgt cggtgggctc ggcgcccggg
     2521 cgccagacct tgcgcccgac atagatgctg cccaccgaca gcaccgagta gaccaggatc
     2581 tggtactgcc agtcgagcgc gggcagcacc agcaggacca ggccggtgat cccggcggag
     2641 acgccgagcc acaggaagaa ggtgcccggc accagcatct cgagcgccac cagcgccacg
     2701 ccggcgatcc accaatgcca gtactggacc tcggtcaaga agctgtccat cgccaaggct
     2761 cctcagggac tcgtttgcgc cgcgccgctg ttgccgcggc gatcgatcgc ctccttggcc
     2821 agctcggcga tgccggcgac cgcgccgatc acgttcgccg cctcgagcgg catcatgatg
     2881 atcttgctgc tcgaggcgct ggcgatgttg cccagcgcct cgacatattt ctgggcgacg
     2941 aagtagttga tcgcctggac gttgcccttg gagatcgcct ccgagaccat ggtcgtcgcc
     3001 cgcgcctcgg cctcggccga gcgctcgcgc gcctcggcgt cgcggaacgc ggcctcgcgc
     3061 cggccctcgg cctccagcac cgccgcctgc ttgctgccct cggcgcgcag gatttgcgcc
     3121 tggcggtcgc cctcggcgac caggatcacg gcacgccggt cgcgctcggc cttcatctgg
     3181 cgccccatcg actcgaccag gtcggccggc gggctgatgt ccttgacctc gatgcgggtg
     3241 atcttgaccc cccacggatt ggtcgcctcg tcgaccacgt gcaggatgcg ggcgttgatc
     3301 acctcgcgct gcgacaacag ctcgtcgagg tccatcgagc ccatcacggt acgcaggttg
     3361 gtcacggtca ggttgacgat ggcgagctcg aggtcggcga cctcgtaggc cgccttggcc
     3421 gcctcgagca cctggaagaa caacacgccg tcgacccgca ccatggcgtt gtccttggtg
     3481 atgatctcct ggctcggcac gtcgatcacc tgctccatca tgttgagctt ggcgccgacg
     3541 ccgtcgatca ccggcacgat caggttgagg ccgggcctga gcgtgcgggt gtagcggccg
     3601 aaccgctcga ccgtgaactc gcggccctgc ggcaccgact tgacgctcag gaacaccagg
     3661 atcacggcga gcaccaggac gacgacgacg aagatcgtca ggccaatcgg ttgcatggcg
     3721 gcttcccccg gtttgcgcgg cagcgcgccg cggcgcgacg cgacgcggca gtctatcccg
     3781 ttactgagat agcctgcgac cgcgaatttt cgagcgccgg caagccggcc gccggggcgc
     3841 ctagtccggc gacttgacga tgatcacgcc cgacatcagc gggtggacgc cgcacacgta
     3901 ggggatcacc gtctcctcgt cgaactcgat cagcgcggtg ccgccgggca tcaggatgcc
     3961 ggtgttgaac tcgcgctcga ggccgaccgc gctgtgctcg atgccgtgga tattggtgaa
     4021 gtggatgcag tcgcccggcg cgacatagat gtcgccgggc tcgaaggtgt aggtgatcgc
     4081 cacctcgtgg accgccgtgc actcttcggc gcgcgccgcg ggcgcgagcg cgaccaggac
     4141 ggcgaagatc gccgcggccg aagcgacggc gcacttgacg gcggctggac ctggtttcat
     4201 gacgctcgtg cctgctcgac gtttcaaaac gcccgataaa gacaccaagg cggcgcgaat
     4261 cacaaccctt gccgctcata ttgccgacgg tcgtgaggcc gccgccttga tcggggtcaa
     4321 tcggcgcggg tgccagcgag cagcgggcgg atcgcggcgg cgatgtcggc ggccggcgtg
     4381 ccgtgctgga acaggccgag cacgcgcccg tcgggcccga tcagatagct cgccgcggtg
     4441 tggtcgatga cgtagtcgtc cgcgcccggc ccgccatcgc cttcgggctc gaccttgtgg
     4501 acgccgacgc gatagccggc ggcgacggcg gcgatttggg cgggcgagcc ggtcaggccg
     4561 atcaggcgcg gatggaagct gccgacatag gccgcgagcg cctcggccgt gtcgcgcgcg
     4621 gggtcgatgg tgatgaacag cggctggacg cgcgtaccgg gcgcgccgag ccgctcgatt
     4681 gcatcggcga tcaccgcgag ctcggtcgga cagatgtccg ggcaatggct gtagccgaag
     4741 aacagcagca cgaagctgcc gaggaagtcc cggtcggtga cttgccggcc aagatggtcg
     4801 accagcgcga aggcgcgcgg gcttgcggcc ggcgcgtcct cggcaagcgc gccgcaggcc
     4861 ggccacaaga ccgcggtcgc gaccacggcg gcgaggaccg cgcgagcttt catggcgtcg
     4921 gcctcctcgg ctgtcggcgc gggcgatgtc gccccgcctg ccagcctagc acggccggcg
     4981 gcgggcgaat gtggtagtgg gaggcggagc ggccttgtta ccatccgcgc ggcgcggcgc
     5041 aaccggacgg ggaagcagtg agcgagcgat ttgcggccat gcgtcacctg cgccggggtc
     5101 gggtcgcgct ggcggtggcc gtggctgcgg cggcggtcgg tgccccggcg cgcgccggca
     5161 tggacgaacc gctcgccacc cggctcgcgc ccgagctggt cgcggagatg ttcccggatg
     5221 ccgagtcgcc gcccgcggtg tcgggcgatc cgccgatcgc gaccgtgcgc cgcgccggcg
     5281 agctcgccgg ctacctgttc tcgactcacg agatggtcca tcccgcgggc tacgccggca
     5341 actcgttcga catcgtcgtc ggcctcgatg cgcacggcgt gatcctgggc aaccggctgc
     5401 ttgaggagca cgagccgctg atcgggccgg gcatgatcac gccgtcgagc ttccgcaggt
     5461 tcctgggcga gctgaccggg gtcgacatca agaccacccg ccggttcgtg cccaaggggg
     5521 tcgatggcgt ctccggcgcc acggtcagcg ccattatcat gggcgacgcg atcctcgatt
     5581 cggcgctgcg cgcgggccac ctgaccggcc tcatcggcgc cggcgacgaa ggcctggcga
     5641 tcgaccggtt cagcttcgag cccgagagct gggccgggct ggtcggcgac ggctcgatcg
     5701 cctcgctgtc gctgaccaac gccgaggtgc gtgctgcgtt cgccgaggcg ttcggcgccg
     5761 acgccgcgcc ggagctcggc gccgaccagg cgccgttcat cgccctccac gtcgcgctgg
     5821 cgacgccgcc caggatcggg cgcaacctgt tcggcgcgcg cgcgttccgc cagatcgccc
     5881 aggacgcgag gcccggcgag cagcagctgc tgatcgcctc gaccggcccc tatcactggc
     5941 tgccggccaa cccctatctg gtggcggcgt tcgaccggct gcacatcgtc cagcgcggga
     6001 ccgtgctgga gatacggccc gagaacttca atcccgcgcg ccggctcgcg gtcgaggacc
     6061 agccgcgctt caccaatgcc ggccggctca gcatcgcgcc cgaggcgggc ttcgatccct
     6121 tggcgccgtg gacgctcgag ctgctggtcg atgagcgcgc ccaggacggc gccgcgccgc
     6181 gcgcgctcgc cttcgcgctg ccctatctcc tgcccgcacg ctacgtgctc ggcgacgagc
     6241 gcgcactcga ggacgcgggc tacaggacgc cgcgctatgt cggcttcggc ctgttccgcg
     6301 acagcacgct caacggctgg cagcgggtct gggccgacaa ggcgggcgcg atcgcgggcc
     6361 ttgtggcact gctcgccgcg gtcacggcgg tgatgctggt ccagcaccgg ctgacccgcc
     6421 atcgccgcct gtatgccctg gtccgcaccg gcctgctcgc ggcgaccctg gtctggctcg
     6481 gctggatcgc gggcgcgcag ctcaccgtcc tgaccgtgct gtcctatttg cgcctgccgt
     6541 tcgatgccgg cggctggcgg tcgctgctgt tcgacccgct gctggtgatc ttgaccggct
     6601 acgttgcggt gtcgctggtg ctgtggggac ggggcgtgtt ctgcggctgg ctatgcccgt
     6661 tcggcgccct gcaggagctg ctcaacaagg ccgcgcggct gcttggcgcg ccccagctcc
     6721 aggttggcga gccggtgcag cgccggctgt gggcgatcaa atacgtcgtc gccatcggca
     6781 tcgtcgcgct ggccgcctat tcgctgagca cggcaacggt tgccgccgag gtcgagccgt
     6841 tcaagaccgc gatcacgctc ggtttccggc gctcgtggcc gttcgtcgcc tatgccggcc
     6901 tgctgctcgc ggcggggctc agcatcgagc gcttcttctg ccgctacctg tgcccgctcg
     6961 gcgcgctgct cgcgatcggc ggccggctgc acctgttcga gtggctcgcg cgccgcgccg
     7021 aatgcggcag cccgtgccag atctgccgcc agtcctgccc ggtcggcgcg atccggcgca
     7081 gcggcgcgat cgacatggac gagtgcctgc aatgcctcga ctgccaggtc gactatcacg
     7141 atccgcaccg gtgcccgccg ctggtcgcgc tccgcaagca cgccgtggag gcggcggtcg
     7201 ctgccgcata gcgcccgcca acggcggact cgtccgcccc ggcgaattct gttacgattt
     7261 tggcgatgcg gatgcgagcc ggcggtcaca ccggcgccgg ctctaccccg gggagcggcc
     7321 gtaaggtcgg gatcgggccg ctccgcaggg gtcgtttccg cgacccgtaa gcacacaaca
     7381 acggtgggga ggacgccaga tggaattcaa tttcctaaac tatttcaagg acttcggtga
     7441 tgagcgggtg agcctgaccg atccttacca gcccggcacc gccttcttcc gcagcggcgg
     7501 tctgggcaag aaggtgcgtg cgcggatgga cgacatggct gacaagtcgg agctgccgcg
     7561 gcgcgagttc ttcaagtccg cgctgggctt caccggcgcc atgctggcgg tcaacgccgc
     7621 caccggcatg cgcttcttcg aggtctccga ggccgaggcc cgggagttcg ccgcgctcga
     7681 ggaggtcggc gagtcgatca agggcaagat gggcgcggtc atcgacatgc acacccacgt
     7741 gtgcacccgc ccggaaggct atgtcgaggg tgtcaacacc accaagcagg gcatgtggtt
     7801 cgtgcgcctg ctcgacgacc tcggcaaggc gttcggtctc gagaacggca ccgccgacat
     7861 gaacgtcgag aactacggca agatgctgct cgagggcagc gacaccgaca tcggcgtgtt
     7921 caacccgttc gggttccgcg aggattacgg cggcaaggac atggtgccga tcgagtacca
     7981 ggcccaggtc aagaagaact ggccgtcgca gaccatcatg ctcgccggcg gcctgacccc
     8041 caatcagggc gtcagcgaga cgctcgaccg gctcgagatg tacgtcaagg actacaaggt
     8101 ctcgggcctc aagctgtaca ccttcgactc gaccaaggcc aagggctggt ggttcgacga
     8161 cgaggagcgc gcctacccga tctgggagaa gtgccgcgag ctcggcatca agaacatcgg
     8221 ctgccacaag ggcatcccgt tcggccagtt catggcgcgt tacgcccatg ccgaggactt
     8281 cgacaaggcg tgcgacgact tcctcgacct caacttcatc gccttccact cggcgtggcc
     8341 gtatcacggc gagctggcgg cgctgaaggg cttcaagccg cagcgcaaca acatgttcag
     8401 cgaggtcggc tcgaccttcg cggcgaccgt gaccagccgg ccggtcgagt gcgcgcacgt
     8461 gatcggcacc ctggtccgcg acctcggcgc cgactacgtg atgtggggca ccgactcgct
     8521 gttgtggggc aacccgcagt ggcagatcga cgccttccgc cgcttccgga tcccggacga
     8581 gctgatcgac ggctacggct atccgcagct gaccgacgag gtcaagacca agatcttcgg
     8641 cggcaacgcc gcccggctgt ggggcctgaa gacggcgatg aacgacaagg gacgccaggt
     8701 cccggtggtc gacttcggct gagcacgagc gatcgcgcgg gacggccggc ggccccggcc
     8761 gtcccgcatc ttttctgagg ctccagatca ggcggcggat gtccgatgcc cacctatgag
     8821 tacaaatgcg agccgtgccg gaccatctat aaggtgcatc acggcatgaa cgagacgccg
     8881 gtgatccgct gtcccgagtg caacaccaag accgagcgca tgatctcggc gcccaacctc
     8941 agcctcggcg gctattccag cccgaccgag gccaagtacg ccaagatgtc ggagcgcgac
     9001 gaggtcgcgc gcgagaacga gctgcagaag acctacaaga ccatctggct gccggaagag
     9061 gtcaagcacg ccccgccctg ggccgacgac gaccaccatc actgacccgc aaccgtcatt
     9121 cgactcatac caaatcgcgt tgatgctgac ccattcttcg actgtcatcg cgagccgcga
     9181 agcggcgtgg cgatccagga aatagtttgc attgccaaag gttctggatt gcgtcgtcgg
     9241 ctccgcctcc tcgcaatgac gatccccgac gggtcaatac ctgcgcgatt tggtattaac
     9301 cgttttccgg attgccgcgc gccggctccg cgcctgcgcg tgcctcaagt ggcgggcgac
     9361 cgttcagcga cgatcgctca ggtgccggcg cgcggcaggt ggacggtggc gcgcaggccg
     9421 ccttctgcgc ggttctccaa ggtgatgtcg ccgccgtggt tgcgcacgat ggtgcgcgcg
     9481 atggcaaggc cgaggccgat gccgccggtg tcgcggctgc gcgagggctc gagacggacg
     9541 aacggcgcga acgcgcgctc gcggtcggcg ggcgcgatgc ccggcccgtc gtcgtcgatc
     9601 accacccaaa gctccgccgc gtcgccgtcg aggcttaccc gcgcgcgccg gccgtaggtc
     9661 gcggcgttgt cgatcaggtt gcgcagcgcg cgcctgagcg cgcccggccg gcacgggatc
     9721 ggggtgcgcg cggcgccggc gaagtcgac
//
LOCUS       DASWHU010000149         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2657599, whole genome shotgun sequence.
ACCESSION   DASWHU010000149 DASWHU010000000
VERSION     DASWHU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657599"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..2213)
                     /locus_tag="VGB88_05740"
     CDS             complement(390..2213)
                     /locus_tag="VGB88_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX9646978.1"
                     /translation="MTIAFRLLTSAAVVAVTLAAPAGAQAPAEPRYQVVVERDVMVAM
                     RDGTRLAIDVWRPDAAGTFPALVERTPYDKTNSSEIVVGAHTFFAERGYVFLVEDVRG
                     RYASEGDFYPFRDSGWQDRRDGFDTLAWIAAQPWSNGKVGVIGGSHTGQTAYMLAPTQ
                     PPALAAMFVRESAADLHKHWVWRGGAFEHGFVTAWVARTFAKNVIERAYAGDRRSHAL
                     AGIQRFLDSQDNYFWYLPVNRFPAFRDLPELSFYDDWLANAADGPYWWRQNVALNHHL
                     FTVPVAHLGGWYDIFLQGTLENYLGLTARAQSALARANQKLVIGPWVHGPTNVGVTEV
                     GALTFPGADDVQYNDVRLHWFDHWLRGIDTGAAAEPPVLIYVMGENRWRAEEAWPLAR
                     ARSTKLYLHDGPSGTLASLNDGGLSATPPGAFERPDSFRYDPDRPVPTLGGNTLFIAS
                     GPQDHRAADELSLTYTSDVLAEDLEVTGPVTAVLYAMSSAVDTDWTVRLSEVYPDGRS
                     INIVDGIQRARYRDDPAAATLIEPGRLYRYDIDLWATSNLFRAGNRLRVSVHSSNFPR
                     WSRNLNVADSPEAATKPEIAYNTVLHDAAWPSHIVLPVIPR"
     gene            2460..3098
                     /locus_tag="VGB88_05745"
     CDS             2460..3098
                     /locus_tag="VGB88_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX9646979.1"
                     /translation="MTRLSRRRLIGALAAGGALAGAAPVRAASCLLTPDQATGPFYPV
                     AFGESDADLTRVAGASLRAEGKVIEVTGAVLGADCRPLAGAVIELWQANAAGRYAHPR
                     EAGNARALDPGFQGAARLTAGADGAYRLVTIKPGAYPATGSWWRPPHIHFRVVAPDGH
                     AFVTQMYFAGEELNEADRLYGSIPVELRDRVTVAFDARADGVAAGQFDIVLG"
     gene            3111..>4026
                     /locus_tag="VGB88_05750"
     CDS             3111..>4026
                     /locus_tag="VGB88_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9646980.1"
                     /translation="MAGRLRLATFNLETLAADGPGVLTLEERAALLRPQLARLRADVL
                     CLQEVNARELADGSRDLAHLDQLIEGTPYAGFERAVTAGAHGKLLDRHNLVVLSRYPI
                     AHAGQLRHDLVPPLTLRRVTGEGAPADYKIEWDRPAQQVTLQLDEATRLHLFNLHLRS
                     RLASLIPGAKTGPLSWASAGAWAEGAYFSAVKRTGQALEVRLSIERLFDAEAEPLIAV
                     AGDLNSEADEMAVEILCADTDNVANPHLADRALALLHGRGDEASNVSMLFHGRALRPD
                     HILVSRALLGWTRELEVHNELLHDESVAL"
BASE COUNT          569 a         1418 c         1447 g          592 t
ORIGIN      
        1 cgccgattat tcgattcacg cggcgacgcg gaggacgcgg cggtcgcgtt ccgtgccagg
       61 ctaacttatc gctccctaga gtctgatacc aacgagccaa ccaattgact cgccggcgcc
      121 cgtcgacacc aatgcgtcac cccgggcgag gcggagccga gacccggggt ccaggatggc
      181 gcgaagcgcc gtcggcgcct gggggtccgg ccaccggatt acgcggccct ggattccggc
      241 tctccgctcc gctccggccg gaatgacggc atgggcggcg tcgttcatat gctcgctggt
      301 atgagcctcc cgccgcttgc ggggagaggt aggaagaagc cgcacgtccg cgccctccgt
      361 ggctccgcgt gcccgatcaa gtcctgcact cagcgcggga tcaccggcag cacgatgtgc
      421 gacggccagg cggcgtcgtg gagaacggtg ttgtaggcaa tctcgggctt ggtcgcggcc
      481 tccgggctgt cggcgacgtt caggttgcgg ctccagcgcg ggaagttgct gctgtggacg
      541 ctgaccctga gccggttgcc ggcgcggaac aggttgctgg tcgcccacag gtcgatgtcg
      601 tagcgataga gtctgcccgg ctcgatcagc gtcgccgcgg ccggatcgtc gcggtagcgc
      661 gcgcgctgga tgccgtcgac gatattgatc gagcgcccgt ccgggtagac ctcgctgagc
      721 cgcaccgtcc agtcggtatc gaccgcggac gacatcgcgt acagcaccgc ggtcaccgga
      781 ccggtcacct cgaggtcctc ggccagcaca tccgaggtgt aggtcaggct gagctcgtcg
      841 gcggcgcggt ggtcctgcgg gccgctcgcg atgaacaggg tgttgccgcc gagcgtcggc
      901 accggccggt cggggtcgta gcggaagctg tcgggccgct cgaacgcgcc cggcggggtg
      961 gcgctcaatc cgccgtcgtt gagcgaggcg agggtgccgc tcggcccgtc gtggagatag
     1021 agcttggtcg agcgggcgcg ggcgagcggc cacgcctcct ccgcgcgcca ccggttctcg
     1081 cccatgacgt agatgaggac cggcggctcg gccgcggcgc cggtgtcgat gccgcgcagc
     1141 cagtggtcga accagtgcag gcgcacgtcg ttgtattgca cgtcgtcggc gcccgggaag
     1201 gtcagcgcgc cgacctcggt cacgccgaca ttggtcggcc cgtgcaccca cggcccgatc
     1261 accagcttct ggttggcgcg cgcaagcgcg ctctgggcgc gggcggtgag gccgagatag
     1321 ttctcgagcg tcccttgcag gaagatgtcg taccagccgc cgagatgcgc gaccggcacc
     1381 gtgaacaggt ggtggttgag cgcgacgttc tgccgccacc agtacggccc gtccgccgca
     1441 ttggcgagcc agtcgtcgta gaacgacagc tcgggcaggt cgcggaacgc cgggaagcgg
     1501 ttgaccggca ggtaccagaa atagttgtcc tggctgtcga ggaagcgctg gatgccggcg
     1561 agcgcatggc tgcggcggtc ccccgcatag gcgcgctcga tgacgttctt ggcgaaggtg
     1621 cgcgccaccc aggcggtgac gaagccgtgc tcgaacgcgc cgccgcgcca gacccagtgc
     1681 ttgtggagat ccgcggccga ctcgcgcacg aacatggcgg cgagcgccgg cggctgggtc
     1741 ggcgccagca tgtaggcggt ctggccggtg tgcgagccgc cgatcacgcc gaccttgccg
     1801 ttcgaccacg gctgggcggc gatccaggcg agggtgtcga agccgtcgcg ccggtcctgc
     1861 cagcccgagt cgcggaacgg gtagaagtcg ccctccgagg cgtagcgccc gcgcacgtct
     1921 tcgaccagga acacgtagcc gcgctcggcg aaaaaagtgt gagcgccgac cacaatctcg
     1981 gacgagttgg tcttgtcata gggcgtgcgc tcgaccagcg ccgggaaggt gccggcggcg
     2041 tcgggccgcc acacgtcgat cgcaagccgc gtgccgtcgc gcatcgccac catcacgtcg
     2101 cgctcgacca cgacctggta gcgcggctcg gccggcgctt gcgcgcccgc cggcgcggcg
     2161 agggtcactg caaccacggc cgcgctcgtc agaagcctaa aagcgatggt catgtccgtc
     2221 ctccctgggg acttccattc gaccattggc ggaagcggcg gcgcaaggtt ttggcacgcg
     2281 gagtcgcggc cttcgagacg ccctcttgcg agggctcctc agactgagga gcgccgaagg
     2341 cgcgtctcga agcctccgcg cctccgcgtg ggctcataaa gggccgttca agcacacggc
     2401 gctttgccgg tgcgggcggg cgtggaatca tggccgcgga ccagcaacgg gaggctcaca
     2461 tgacccgact gtcgcggcgc cggctgatcg gcgcgctcgc cgcgggcggg gcgctggccg
     2521 gcgcggcgcc ggtgcgcgcc gcgtcctgcc tgctcacccc cgatcaggcc accggcccgt
     2581 tctatccggt cgcgttcggc gaaagcgacg cggacctcac ccgcgtcgcc ggcgcgagcc
     2641 tgcgtgccga gggcaaggtg atcgaggtga cgggagccgt gctcggcgcc gactgccgac
     2701 cgctggccgg cgccgtcatc gagctgtggc aggcgaacgc cgccggccgc tacgcccatc
     2761 cgcgcgaggc cgggaacgcg cgcgcgctcg atcccggctt ccagggcgcg gcgcggctca
     2821 cggccggcgc cgacggcgcg tatcgcctgg tcacgatcaa gcccggcgcc tatcccgcga
     2881 ccggctcttg gtggcggccg ccgcacatcc acttccgcgt cgtcgcgccc gacggccacg
     2941 ccttcgtcac ccagatgtac ttcgccggcg aggagctgaa cgaggccgac cggctgtacg
     3001 gctcgatccc ggtcgagctc cgcgaccggg tgacggtggc gttcgacgcg cgcgccgacg
     3061 gcgtcgccgc cgggcagttc gacatcgtgc tcggctgacg ggccgcgcgc atggcggggc
     3121 gcctgcggct cgcgaccttc aacctcgaga ccctcgccgc cgacggcccc ggcgtgctca
     3181 cgcttgagga gcgcgcggcg ctgttgcgcc cgcagctcgc gcgcctgcgc gccgacgtct
     3241 tgtgtttgca ggaggtcaac gcccgcgaac ttgccgacgg cagccgcgac ctcgcccatc
     3301 tcgaccaact gatcgagggc acgccctatg ccggcttcga gcgcgcggtg accgcgggcg
     3361 cccacggcaa gctgctcgac aggcacaatc tggtggtctt gagccgctat ccgatcgcgc
     3421 acgccggcca gctccgccac gatctggtgc cgccgctgac cttgcgccgg gtcacaggcg
     3481 agggcgcgcc cgccgactac aagatcgagt gggaccggcc ggcgcagcag gtcacgctcc
     3541 agctcgacga ggcgacccgg ctccatctgt tcaacctgca ccttcgctcg cgccttgcga
     3601 gcctgattcc cggcgccaag accggtccgt tgagctgggc ctcggcgggc gcgtgggcgg
     3661 aaggcgcgta cttctcggcg gtcaagcgca ccggccaggc gctcgaggtg cgcctctcaa
     3721 tcgagcgcct gttcgacgcg gaggccgagc cgctgatcgc ggtcgcgggc gacctcaact
     3781 cggaggccga cgagatggcg gtcgagatcc tctgcgctga caccgacaac gtcgccaacc
     3841 cgcacctcgc cgaccgggcc ttggcgctgc tgcacgggcg gggcgatgag gccagcaatg
     3901 tctcgatgct gttccacggc cgcgcgctcc gccccgacca catcctggtg tcgcgcgcgc
     3961 tgctcggctg gacccgcgag ctcgaggtgc acaacgagct gctgcacgac gaatccgtcg
     4021 cgctcg
//
LOCUS       DASWHU010000150        10210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1550249, whole genome shotgun sequence.
ACCESSION   DASWHU010000150 DASWHU010000000
VERSION     DASWHU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550249"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VGB88_05755"
     CDS             <1..787
                     /locus_tag="VGB88_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9646981.1"
                     /translation="LGAFALTEPGAGSDAAAIRTRAEMVGNQWRLNGTKQFISSGSTA
                     DVAIVFAVTDPDAGKKGISAFLVPTDTPGWQVARIEHKLGQRASDTCQIVLDGVELAP
                     ELMLGAPGEGYRIALSNLEGGRIGVAAQAVGMARAAYEAALAYAKARESFGKPIIEHQ
                     AVAFRLADMATGIEAAHNMVLAAAARRDAGLPCLKEASMAKLFASETAERVCRDAIQV
                     HGGYGYLADYPVERIYRDVRVCTIYEGTSDIQRLVIARAIAGD"
     gene            787..1644
                     /locus_tag="VGB88_05760"
     CDS             787..1644
                     /locus_tag="VGB88_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9646982.1"
                     /translation="MPLASLVDGHFHLWRRAALRVPGIVADPRLDRDIRWPDYVAAFA
                     DLPVTAAVAVQVDGDPGDGEPEVERLEQVAGRHPALAAIVAWAPLEADDVALHLARLA
                     RHPLVRGIRRNTQDEPDPRFCARAEFVRGVRALADYGFACDVCVRHWQLDGALALARA
                     CPETTIALDHIGKPDVAHGTLDPWRERISALADLPNVACKLSVVVHGRPDDRWDEAAV
                     APFAAHVLDRFGPERVLWGSNWPVAGLVTGYRDWAALAERLCARLAAADRAAIFSATA
                     RRVYRIERA"
     gene            1717..3324
                     /locus_tag="VGB88_05765"
     CDS             1717..3324
                     /locus_tag="VGB88_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9646983.1"
                     /translation="MTVLQTVLDPRSSAFRGNADRMVALVQDLWTKIAEVEKGGGEAA
                     RERHLGRGKMLPRERVRLLLDPGSPFLELSQLAAWGMYNDEAPSAGVITGLGRIGGRE
                     CVIVANDATVKGGTYFPMTVKKHLRAQEIARDNRLPCIYLVDSGGGNLPHQDDIFPDR
                     EHFGRIFFNQATMSALGIPQIAVVMGSCTAGGAYVPAMSDETVIVRNQGTIFLGGPPL
                     VKAATGEVVSAEELGGADVHARVSGVADHYAVNDPHALALARRIVASLNRPKTVAVEV
                     SAPQAPLYDAHDIYGLVPEDLRHPFEMREVIARIVDASVFDEFKHLYGQTLLCGFARI
                     CGYPVGILANNGILFSESALKGAHFIELCCQRRIPLVFLQNISGFMVGRKYEAGGIAK
                     DGAKLVTAVACAQVPKFTVIVGGSFGAGNYGMCGRAYGPRFLWMWPNARISVMGGEQA
                     AMVLATVRREALEGRGETWTKDDEAAFRAPILEQYERQGHPYYASARLWDDGVIDPAD
                     TRMVLGLGLSAALNAPIPEPAFGVFRM"
     gene            3327..4127
                     /locus_tag="VGB88_05770"
     CDS             3327..4127
                     /locus_tag="VGB88_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9646984.1"
                     /translation="MAGEPGAGVRLERDGRGIARVTLDRARVHNAFDDSLIARLDEAF
                     AALETDPAVRAVVLAAEGDSFSAGADLTWMRRMAGLGTDDNLADAMRLGELLRRVAHL
                     PKPVIALVQGPAYGGGVGLVAAADIAIASERASFMLSEVRLGLVPAVISPYVVAAIGA
                     RQARRVFVTAERIDAAAALRLGLVHQVVPAAELEAAGARLLERLAANAPGAMAAAKEL
                     VFAVAGRAIDQALIADTARRIAEARGSREGIEGIAAFLDKRKPRWAGG"
     gene            4167..6215
                     /locus_tag="VGB88_05775"
     CDS             4167..6215
                     /locus_tag="VGB88_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX9646985.1"
                     /translation="MFERILIANRGEIACRIIDTARRLGVRTVALYSDADAGARHVRL
                     ADEAYPIGKAPAADSYLRIESVIEVARHAGAEAVHPGYGFLAENPSLAEACAVAGLAF
                     IGPSADAIRAMGAKDRAKQLMEQAGVPVVPGYHEHSRDLKVLTTQARLIGFPILVKAV
                     AGGGGRGMRTVSRELDLAEAIAAARREAQAAFGDDRVLLEKVVARARHVEIQVFADGH
                     GNIVHLYERDCSIQRRHQKVIEEAPSPALSPELRLAMGEAAVRAAEAIGYLGAGTVEF
                     LLDSSGGFHFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEPLPLAQDQIELFGHA
                     LEARLYAEDPRKEFLPSTGRLVHLRLPGVPGASPAGARVPVRIDTGVDQGDEVTPYYD
                     PLIAKIVVWGRDRAEALRRMTRALADTELVGPATNLEFLSAVVGHPAFVANEVDTGFV
                     LRHREELVAEPAPAPALALALAALYLLLDRRRRTAAEAARTADPYTPWAGADGWRLND
                     AAHETVTLIDNGAPVPVEVRTDRRGTRLVLPDGEVGARASFTADGRLSAAVGGIAVTV
                     SVIDHVDARGERQLTVIVQDATYRLGVEDPLAVAAHQDAEGGQLTAPMPGRVTQVLAE
                     DGARVARGAPLLVIEAMKMEHTVAAPASGQVTKVKYAVGDWVGEGESLLDFVAEDGGG
                     "
     gene            6212..7135
                     /locus_tag="VGB88_05780"
     CDS             6212..7135
                     /locus_tag="VGB88_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9646986.1"
                     /translation="MTAAGTLPARVRMFEVGPRDGLQNQPGEVATAVKVELIERLAAA
                     GLTEIEATSFVSPKWVPQMADNAEVMRAIRRRAGVHYPVLTPNMRGFDDALAAGADTV
                     CVFTAASETFSRRNTNCSIADSLARIEPIAEAAARNGVRMRGYISCVLGCPYEGEVAP
                     TIVADIAARLDALGAYEISLGDTVGFGTPLKARALVGAVAERVPVARLAAHFHDTYGQ
                     ALANLLAVLETGVAVIDSSVGGLGGCPYARGAKGNVATEDVLYMLDGMAIETGVDLDA
                     VVAVAWWIFDRLGRRPESRVALARGAAGESA"
     gene            7132..7461
                     /gene="nrtS"
                     /locus_tag="VGB88_05785"
     CDS             7132..7461
                     /gene="nrtS"
                     /locus_tag="VGB88_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HEX9646987.1"
                     /translation="MNLHRRLSARARRRNGQVEADRPGGKDRSENGLSMSKLSTVCRC
                     CVADGIPRRSLAVALVVGAILNSINQGDSILGRGDVDLTKAILTFLVPYGVATYGAVA
                     YRLREPR"
     gene            complement(7468..8127)
                     /locus_tag="VGB88_05790"
     CDS             complement(7468..8127)
                     /locus_tag="VGB88_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9646988.1"
                     /translation="MIQPASLTGPERPPLRGGAARHLVVLLHGLGADGNDLIGLADAW
                     AGLVPDAAFLAPDAPEPCDLAPMGRQWFSLQSRTPVTLLAGARRAAPVLEAYLERQCA
                     RFGVAARRLALVGFSQGTMMALHVALRRAQPVACVLGYSGALVGAESLAGEVRSRPPV
                     LLVHGDADQVVPIGALHGALDALTRAEVAAEWHICRGTGHGIDPQGLALGGRFLAERL
                     A"
     gene            8283..9134
                     /gene="gluQRS"
                     /locus_tag="VGB88_05795"
     CDS             8283..9134
                     /gene="gluQRS"
                     /locus_tag="VGB88_05795"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX9646989.1"
                     /translation="MTTASSPVTRFAPSPSGFMHLGHAYAALFAWHAARRGHGRFVLR
                     IEDIDRARSRQQFADAILEDLAWLGLDWDGPVRIQSEHMADYAAALGRLDELGVIYPC
                     FCTRREIRQEIKHADEAPQGPDEQVYPGTCRELSFDDRRQRIRLGTPHAWRLDVQKAL
                     EITGPIEWRDRAAGLQRAEPLSHGDVVVARKDVPTSYHLAVTIDDCLQGVTLVTRGDD
                     LFHVTGIHRLLQALLGIQAPVWHHHPLITDSSGVRLAKRNRAMTLRALRRAGHSPAQV
                     RAMVGFE"
     gene            9217..9855
                     /locus_tag="VGB88_05800"
     CDS             9217..9855
                     /locus_tag="VGB88_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9646990.1"
                     /translation="MTDVIVYGNPLSTYTRTARMALTAKGVVHTLEPVDHRAPEYRAI
                     QPFGKTPALRHGDFVLYETLAIACYVDEAFDGPPLAPADAQARARMFQWISATLDYFY
                     DAMIVKLVFERLVAKMKGRAPNEALIAEALPDITLQVGVLDKALNGAPYLAGETMSIA
                     DMFLLPILFYVNFTNEGQRILAGAPALGEWLARMSGEPCAEATMPPVAKLTA"
     gene            complement(9866..10063)
                     /locus_tag="VGB88_05805"
     CDS             complement(9866..10063)
                     /locus_tag="VGB88_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9646991.1"
                     /translation="MPRILEAFAQRGLVPARLHAVGDERGELAVDLQVGGLDAETAER
                     VAARLRALVDVRSVLTYAKRA"
BASE COUNT         1404 a         3718 c         3687 g         1401 t
ORIGIN      
        1 gctcggcgca ttcgccctca ccgagcccgg cgcgggctcg gacgccgccg ccatccgtac
       61 ccgcgccgag atggtcggca accagtggcg gcttaacggc accaagcagt tcatcagctc
      121 gggctcgacc gccgatgtcg ccatcgtgtt cgcggtcacc gacccggacg ccggcaagaa
      181 gggcatcagc gcgttcctgg tgcccaccga tacgcccggc tggcaggtcg cgcggatcga
      241 gcacaagctc ggccagcgcg cctccgacac ctgtcagatc gtgctcgacg gcgtcgagct
      301 cgcgcctgag ctgatgctcg gcgcgcccgg cgagggctac cggatcgcgc tttcgaacct
      361 cgagggcggg cgcatcggcg tcgccgccca ggcggtcggc atggcgcgcg cggcctatga
      421 ggcggcgctc gcctacgcga aagcgcgcga gagcttcggc aagccgatca tcgagcacca
      481 ggcggtcgcc ttccggctcg ccgacatggc gaccggcatc gaggccgcgc acaacatggt
      541 gctcgccgcc gccgcccgcc gcgacgccgg cctgccctgc ctcaaggagg cgtcgatggc
      601 caagctgttc gccagcgaga ccgctgagcg cgtctgccgc gacgcgatcc aggtccacgg
      661 cggctacggc tacctcgccg actacccggt cgagcgcatc taccgcgacg tgcgcgtgtg
      721 cacgatctac gagggcacca gcgacatcca gcgcctggtc atcgcgcgcg cgatcgccgg
      781 cgactgatgc cgctcgcctc cctggtcgac ggccatttcc acctgtggcg gcgggcggcc
      841 ttgcgggtgc cggggatcgt cgccgatccc cgccttgacc gcgacatccg ctggcccgac
      901 tacgtcgcgg cgtttgccga cctgccggtc accgcggcgg tcgcggtgca ggtcgacggc
      961 gaccccggcg acggcgagcc cgaggtcgaa cggctcgagc aggtcgccgg ccggcatccc
     1021 gcgctcgccg cgatcgtcgc ctgggcgcca ctcgaggccg acgacgtcgc gctccacctc
     1081 gcccgcctcg cccgccatcc gctggtgcgc ggcatccgcc gcaacaccca ggacgagccc
     1141 gacccgaggt tctgcgcgcg cgcggagttc gtccgtggcg tgcgcgcact cgccgactac
     1201 ggcttcgcct gcgatgtgtg cgtgcgccac tggcagctcg acggcgcgct ggcgctggcg
     1261 cgggcctgcc ccgagaccac catcgcgctc gaccacatcg gcaagcccga cgtcgcccac
     1321 ggcacgctcg atccctggcg cgagcggatc agcgccctcg ccgatctccc gaacgtggcc
     1381 tgcaagctgt cggtggtggt ccacggccgc cccgacgacc gctgggacga ggccgcggtg
     1441 gcgccgttcg ccgcgcatgt cctcgaccga ttcgggccgg agcgggtgct gtggggcagc
     1501 aactggccgg tggcgggcct ggtcaccggc tatcgcgact gggcggcgct cgccgagcgg
     1561 ctgtgcgcgc gcctggcggc ggccgatcgc gcggcgatct tctccgccac cgcccggcgc
     1621 gtctatcgca tcgagcgtgc ctgagcgcgg cctttgcctg tggagtgcgc gcccggcttt
     1681 gcttatagat agggccacga ttcgggggcc cggaccatga ccgtgctgca gaccgtgctc
     1741 gatccgcgct cgtccgcctt ccgcggcaac gcggaccgca tggtcgcgct ggtccaggac
     1801 ctctggacca agatcgccga ggtcgagaag ggcggcggcg aggcggcgcg cgagcgccac
     1861 ctcgggcgcg gcaagatgct gccgcgcgag cgcgtgcgcc tgctgctcga tcccggctcg
     1921 ccgttcctcg agctgtccca gctcgccgcc tggggcatgt acaacgacga ggcgccgtcg
     1981 gcgggcgtga tcaccggcct cggccggatc ggcgggcgcg aatgcgtgat cgtcgccaac
     2041 gacgccacgg tcaagggcgg cacctacttc ccgatgaccg tcaagaagca cctgcgcgcc
     2101 caggagatcg cgcgcgacaa ccgcctgccg tgcatctacc tggtggattc gggcggcggc
     2161 aacctgccgc accaggacga catcttcccc gaccgcgagc atttcgggcg catcttcttc
     2221 aaccaggcga ccatgtcggc gctcggcatc ccgcagatcg cggtggtcat gggcagctgc
     2281 accgcgggcg gcgcctatgt gccggcgatg tcggacgaga ccgtgatcgt gcgcaaccag
     2341 ggcaccatct tcctcggcgg cccgccgctg gtgaaggcgg cgaccggcga ggtggtctcg
     2401 gccgaggagc tcggcggcgc cgacgtgcat gcgcgcgtct cgggcgtcgc cgaccactac
     2461 gcggtcaacg acccgcatgc gctggcactc gcgcgccgga tcgtcgccag cctcaaccgg
     2521 cccaagacgg tggcagtcga ggtgtcggcc ccgcaggcgc cgctctacga cgcgcacgac
     2581 atctacggcc tggtccccga ggacctgcgc catccgttcg agatgcgcga ggtgatcgcg
     2641 cgcatcgtcg atgccagcgt gttcgacgag ttcaagcacc tgtacggcca gacgctgttg
     2701 tgcggcttcg cgcgcatctg cggctatccg gtcggcatcc tcgccaacaa cggcatcctg
     2761 ttctcggaat ccgcgctcaa gggcgcgcat ttcatcgagc tgtgctgcca gcggcggatt
     2821 ccactggtct tcctgcagaa catctccggc ttcatggtcg ggcgcaaata cgaggccggc
     2881 ggcatcgcca aggacggcgc caagctggtc accgcggtcg cctgcgccca ggtgcccaag
     2941 ttcacggtca tcgtcggcgg cagcttcggc gccggcaact acggcatgtg cgggcgcgcc
     3001 tatggcccgc gcttcctgtg gatgtggccg aacgcgcgca tctcggtgat gggcggcgag
     3061 caggcggcga tggtgctggc gaccgtgcgc cgcgaggcgc tcgaggggcg cggcgagacc
     3121 tggaccaagg acgacgaggc ggcgttccgg gcgccgatcc tcgagcagta cgagcgccag
     3181 ggtcatccct actacgccag cgcgcggctg tgggacgacg gcgtcatcga ccccgcggac
     3241 acgcgcatgg tgctggggct cgggctgtcg gcggcactga acgcgccgat ccccgagccc
     3301 gcgttcggcg tgttcaggat gtagcggtgg cgggcgagcc gggcgcgggc gtgcgacttg
     3361 agcgggacgg gcgcggcatc gcgcgcgtca ccctcgaccg cgcccgggtc cacaacgcct
     3421 tcgacgattc gctgatcgcg cgcctcgacg aggcgttcgc ggcgctggaa acggacccgg
     3481 ccgtgcgcgc ggtggtgctg gcggccgagg gcgacagctt ctcggccggc gccgacctca
     3541 cctggatgcg gcgcatggcc ggcctcggca ccgacgacaa cctcgccgac gccatgcgcc
     3601 tcggcgagct gctccgtcgc gtcgcccatc tgccgaagcc ggtgatcgcc ctggtccagg
     3661 gcccggccta tggcggcgga gtcgggctgg tcgccgccgc cgacatcgcg atcgccagcg
     3721 agcgcgcgtc gttcatgctg tccgaggtca ggctcggcct ggtgccggcg gtgatcagcc
     3781 cctacgtggt ggcggcgatc ggcgcccgtc aggcgcgccg cgtcttcgtc accgccgagc
     3841 gcatcgacgc cgcggcggcg ttgcggctgg gcctggtcca ccaggtggtg ccggcggcgg
     3901 agctcgaggc ggcgggcgcg cgcctgctgg agcggctggc ggccaacgcg ccgggggcga
     3961 tggcggcggc gaaggagctg gtgttcgcgg tcgccggccg cgccatcgat caggcgctga
     4021 tcgcggatac cgcgcgccgg atcgccgagg cgcgcgggag ccgcgaaggc atcgagggca
     4081 tcgccgcgtt cctcgacaag cgcaagccgc gctgggcggg cggctgaggc ccgatggtca
     4141 tcgccggcgg gcaggatggg tgaaaggtgt tcgagcggat tctgatcgcc aatcggggcg
     4201 agatcgcgtg ccggatcatc gacaccgcgc gccggctcgg cgtgcgcacg gtggcgctct
     4261 attcggacgc cgacgccggc gcccgccatg tccggctcgc cgacgaggcc tatccgatcg
     4321 gcaaggcgcc ggccgccgac agctatctga ggatcgagtc ggtgatcgag gtcgcgcgcc
     4381 acgccggcgc cgaggcggtc catccgggct acggcttcct ggccgagaac ccgtcgctgg
     4441 ccgaggcctg cgcggtcgcc ggcctcgcct tcatcggccc gagcgcggat gcgatccgcg
     4501 cgatgggcgc caaggaccgc gccaagcagc tgatggagca ggccggggtg ccggtggtcc
     4561 ccggctatca cgagcacagc cgcgacctca aggtgctgac cacccaggcg cgcctgatcg
     4621 gcttcccgat cctggtcaag gcggtggccg gcggcggcgg gcgcggcatg cgcacggtca
     4681 gccgcgagct cgacctggcc gaggcgatcg ccgccgcgcg gcgcgaggcc caggcggcct
     4741 tcggcgacga ccgcgtgctg ctggagaagg tcgtcgcccg cgcgcgccat gtcgagatcc
     4801 aggtgttcgc cgacggtcac ggcaacatcg tccacctgta cgagcgcgac tgctcgatcc
     4861 agcgccgcca ccagaaggtc atcgaggagg cgccgtcgcc ggcgctaagc cccgagctgc
     4921 gcctggcgat gggcgaggcg gcggtgcgtg cggccgaggc gatcggctat ctcggcgccg
     4981 gcacggtcga gttcctgctc gattcctcgg gcggcttcca cttcatggag atgaacacgc
     5041 gcctgcaggt cgagcatccg gtgaccgaga tgatcaccgg cctcgacctg gtcgaatggc
     5101 agctcagggt cgccgccggc gagccgctgc cgctggccca ggaccagatc gagctgttcg
     5161 gccatgcgct ggaggcgcgg ctatacgccg aggacccgcg caaggagttc ctgccctcga
     5221 ccgggcggct ggtgcacctg cgcctgccgg gcgtgccggg cgcgagcccg gccggcgcgc
     5281 gcgtgccggt gcgcatcgac accggcgtcg atcaaggcga cgaggtgacg ccgtactacg
     5341 acccgctgat cgccaagatc gtggtctggg ggcgtgaccg ggccgaggcg ctgcggcgca
     5401 tgacgcgcgc gcttgccgac accgagctgg tcggtccggc gaccaatctc gagttcctgt
     5461 cggcggtggt cggccatccg gcgttcgtcg ccaacgaggt cgacaccggc ttcgtgctgc
     5521 gccaccgcga ggagctggtg gccgagccgg cgccggcgcc agcgctggcg ctggcgctgg
     5581 cggcgctgta cctgctgctc gaccgccgcc ggcgcaccgc ggcggaggcg gcgcggaccg
     5641 ccgatcccta cacgccgtgg gcgggcgccg acggctggcg gctcaacgac gccgcgcacg
     5701 agaccgtcac cctgatcgac aacggcgccc cggtgccggt cgaggtccgc accgaccgcc
     5761 gcggcacccg gctggtgctg cccgacggcg aggtcggcgc gcgcgcgagc ttcaccgccg
     5821 acggccgcct cagcgccgcg gtcggcggca tcgcggtcac cgtgtcggtg atcgaccatg
     5881 tggacgcgcg cggcgaacgc cagctgaccg tgatcgtcca ggacgccacc tatcgcctcg
     5941 gcgtcgagga cccgctcgcc gtcgccgcgc accaggacgc cgagggcggc cagctgaccg
     6001 cgccgatgcc cgggcgggtc acccaggtgc tggccgagga cggcgcccgg gtcgcgcgcg
     6061 gcgcgccgct gttggtgatc gaggcgatga agatggagca cacggtcgcc gcgcccgcct
     6121 ccggccaggt gaccaaggtc aagtatgcgg tcggcgactg ggtcggcgag ggcgagagcc
     6181 tgctcgactt cgttgccgag gacggcggcg gatgaccgcc gcggggacgc tgccggcgcg
     6241 ggtgcggatg ttcgaggtcg gcccgcgcga cggcctgcag aaccagccgg gcgaggttgc
     6301 gaccgcggtc aaggtcgagc tgatcgagcg cctcgccgcc gccggactga ccgagatcga
     6361 ggcgaccagc ttcgtctcgc ccaaatgggt gccgcagatg gccgacaacg ccgaggtcat
     6421 gcgcgcgatc cgccgccgcg ccggcgtcca ctacccggtg ctgaccccga acatgcgcgg
     6481 cttcgacgac gcgctcgccg ccggcgccga cacggtgtgc gtgttcaccg ccgcgtcgga
     6541 gactttctcg cggcgcaaca ccaactgctc gatcgcggac agcctggcgc gcatcgagcc
     6601 gatcgccgag gcggcggcgc gcaacggcgt gcgcatgcgc ggctacatct cgtgcgtgct
     6661 cggctgccct tatgagggcg aggtcgcgcc gacgatagtc gccgacatcg cggcgaggct
     6721 cgatgcgctt ggcgcctatg agatctcgct cggcgacacg gtcggcttcg gcacgccgct
     6781 gaaggcgcgc gcgctggtcg gggcagtggc cgagcgggtg ccggtcgcgc gcctggccgc
     6841 ccatttccac gacacctacg gccaggcgct cgccaacctg ctggcggtgc tcgagaccgg
     6901 ggtggcggtg atcgacagct cggtcggcgg cctcggcggc tgcccttatg cccgcggcgc
     6961 caagggcaac gtcgccaccg aggacgtgct ctacatgctc gacggcatgg ccatcgagac
     7021 cggcgtcgat ctcgacgccg tggtcgccgt cgcgtggtgg atcttcgacc ggctcggccg
     7081 gcgtcccgaa agccgggtcg cgctcgcccg cggcgccgcc ggcgagtcgg catgaacctc
     7141 catcggcgcc tctcggcgcg agcgcgtcgc cggaacggtc aggtcgaggc cgatcgaccc
     7201 gggggaaagg atcgctcgga aaacgggttg tcaatgtcca aactatctac cgtgtgccgc
     7261 tgctgcgtcg ccgacggcat accgcggcgt tcgctcgcgg tggcgctggt cgttggcgcg
     7321 atcctgaact caatcaatca gggcgattcg atccttggtc ggggcgacgt cgatctcacc
     7381 aaggccatcc tcaccttcct ggtgccgtac ggcgtggcca cctatggtgc cgttgcctac
     7441 cgcttgagag agcctcggta ggcggcgtca cgccagccgc tcggccagga accggccgcc
     7501 gagcgcaagg ccttgcggat cgatgccgtg gccggtcccg cggcaaatat gccactcggc
     7561 cgccacctcg gcgcgggtaa gcgcatcgag cgcgccatgg agcgcgccga tcggcaccac
     7621 ctggtcggcg tcgccgtgga ccagcagcac cggcgggcgc gagcgcacct ccccggccag
     7681 cgactcggcg ccgaccagcg cgcccgagta gccgagcacg caggcgaccg gctgggcgcg
     7741 cctgagcgcg acatggagcg ccatcatcgt cccctggctg aagccgacca gcgccagccg
     7801 ccgcgccgcc acgccgaagc gcgcgcactg ccgttcgaga tacgcctcga gcaccggcgc
     7861 ggcgcggcgc gcgccggcga gcagcgtaac gggtgtccgg ctttgcagcg agaaccattg
     7921 ccggcccatc ggcgcaaggt cgcagggctc gggcgcgtcg ggggcgagga acgccgcgtc
     7981 gggcaccagc cccgcccagg cgtcggcaag cccgatcagg tcgttgccgt cggcgccgag
     8041 cccgtgcagc agcaccacca ggtggcgcgc ggcgccgccg cgcaacggcg ggcgctcggg
     8101 gccggtcagg ctggcgggct ggatcatcgg ttgtgcggcg gcgttggcgg gcgggcggcg
     8161 aggtcttggc gggcggccac tgggcaaccg gcaccgccga tgctatctca ttgtcgtggc
     8221 gcaatgatcg ccgttcaccg cgcgcgccgc aagagccgtt cccgcacccg gaccagcctg
     8281 ccttgaccac cgcttcatcg cccgtcaccc gcttcgcgcc gagcccgagc ggcttcatgc
     8341 atctgggcca cgcctatgcc gcgctgttcg cctggcacgc ggcgcggcgc ggccacggcc
     8401 gcttcgtgct gcgcatcgag gacatcgacc gcgcccgcag ccgccagcag ttcgccgacg
     8461 cgatcctcga ggacctggcc tggctcggcc tcgactggga cgggccggtg cgcatccagt
     8521 ccgagcacat ggcggattat gcggcggctc tggggcgcct cgacgagctc ggcgtgatct
     8581 atccctgctt ctgcacccgg cgcgagatcc gccaggagat caagcacgcc gacgaggcgc
     8641 cgcagggccc cgacgagcag gtctatcccg gcacctgccg cgagctcagc ttcgacgacc
     8701 ggcgccagcg tatccggctc ggcacgccgc atgcctggcg gctcgacgtg caaaaggcgc
     8761 tcgaaatcac cggcccgatc gagtggcgcg accgcgccgc cggtctccag cgcgccgagc
     8821 cgcttagcca cggcgacgtg gtggtggcgc gcaaggacgt gccgaccagc tatcatctgg
     8881 cggtcaccat cgacgactgc cttcagggcg tgaccctggt cacccgcggc gacgacctgt
     8941 tccacgtcac cggcatccac cgcctgttgc aggcactgct cggcatccag gcgccggtct
     9001 ggcaccacca cccgctgatc accgactcga gcggggtgag gctggccaag cgcaaccggg
     9061 cgatgacctt gcgcgcgctg cgccgcgccg gccacagccc ggcccaagtg cgggcgatgg
     9121 tcgggttcga gtaggcttgg gttcgcgggc gcaatccggc tagacttggc ccgaggcgaa
     9181 gccagcccgg caatcgaacg cccgcggaga cgcgccatga ccgacgtcat cgtctacggc
     9241 aacccgctca gcacctacac ccggaccgcg cgcatggcgc tgaccgcgaa gggcgtcgtg
     9301 cataccctgg agccggtcga ccaccgggcg ccggagtacc gcgcgatcca gccgttcggc
     9361 aagaccccgg cgttgcgcca cggcgacttc gtgctctacg agacgctcgc gatcgcctgc
     9421 tatgtcgacg aggccttcga cggcccgccg ctggcgcccg cggacgcgca ggcgcgcgcg
     9481 cgcatgttcc aatggatcag cgccacgctc gactacttct acgatgcgat gatcgtcaag
     9541 ctggtgttcg agcgcctggt cgccaagatg aagggccgcg cgcccaacga ggcgctgatc
     9601 gcggaagccc tgcccgacat cacccttcag gtcggcgtgc tggacaaggc gctgaacggc
     9661 gccccttacc ttgccggcga gaccatgagc atcgccgaca tgttcctgct gccgatcctg
     9721 ttctacgtca acttcaccaa cgagggccag cgcatcctgg ccggcgcgcc ggcgctcggc
     9781 gaatggctcg cgcgcatgag cggcgagccc tgcgccgagg cgaccatgcc gccggtcgcc
     9841 aagctcaccg cctgagcgcg cgcgcttacg cccgcttggc gtaggtcagc accgagcgca
     9901 cgtcgaccag cgcgcgcagc cgggccgcga cccgctctgc ggtctcggcg tcgaggccgc
     9961 caacctggag gtcgacggcg agctcgccac gctcgtcgcc gaccgcgtgc aggcgcgccg
    10021 gcaccaggcc gcgctgggcg aacgcctcga ggatgcgagg caaggtgccc gggcctgcgg
    10081 tcgcggcgac cgagaagcaa tcgacgcgcg cgcaagccgc ggcgcggcct gatcgggcgc
    10141 cgggtgcgct atcttcgggg gatggcatga cggacgtcct tgttcgagcg acgcaaaggc
    10201 ggacaggcga
//
LOCUS       DASWHU010000151         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1929802, whole genome shotgun sequence.
ACCESSION   DASWHU010000151 DASWHU010000000
VERSION     DASWHU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929802"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGB88_05810"
     CDS             <1..648
                     /locus_tag="VGB88_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9646992.1"
                     /translation="IDWQRLPDVDWVSESLSRLDRVTAGRFTVRGSHLAPGRARIELV
                     IDAGQAFGTGHHPTTAGCLEAITHLSRLRRFARPLDLGTGSGVLAMAMARLWRVAVLA
                     VDVDPIAVRAARANARRNRLGPWIEAVVAGGLRAPAVRRRAPFDLVVANILAGPLARM
                     APDLARRLAPGGIAVLSGLLADQQAGVAAAYRAQGLRLVRRDLRQGWATLTVGAR"
     gene            675..1439
                     /locus_tag="VGB88_05815"
     CDS             675..1439
                     /locus_tag="VGB88_05815"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9646993.1"
                     /translation="MDAFDLSGRVAIVTGGNGGIGLGMAAGLARAGASLLIAGRNEDK
                     SRAAVAELEALGATAAAVRVEITDRASVQAMVAAAGERFGRLDILVNNAGTNIRKQPE
                     DYTPEEWHTVIETNLTSAFHCCQAAYRPMVAAGGGKIINIGSMMSIFGASFTMPYASS
                     KGGIVQMTKALACAWAKDNIQVNAVLPGWIDTTLTRQARQQIEGLHERVVARTPAGRW
                     GMPRDFAGIAVFLAAPASDFLTGTAIPVDGGYSVQG"
BASE COUNT          209 a          581 c          618 g          223 t
ORIGIN      
        1 atcgactggc agcgcctgcc cgacgtcgac tgggtatccg agagcctcag ccgcctcgac
       61 cgggtgacgg ccgggcggtt caccgttcgc ggctcgcacc tcgcgccggg gcgcgcgcgg
      121 atcgagctgg tgatcgacgc cggccaggcg ttcggcaccg gccatcaccc gaccaccgcg
      181 gggtgcctgg aggcgatcac gcacctttcc cggctgcggc ggttcgcgcg ccccctcgac
      241 ctcggcaccg gcagcggcgt gctcgccatg gcgatggcgc ggctgtggcg ggtggcggtg
      301 ctggcggtcg acgtcgaccc gatcgcggtc agggcggcgc gcgccaatgc gcggcgcaac
      361 cgcctcggcc cctggatcga ggcggtggtc gccggcggcc ttcgcgcccc ggcggtgcgc
      421 cggcgggcgc cgttcgacct ggtggtcgcc aacatcctgg ccgggccgct ggcgaggatg
      481 gcgccggacc tcgcccgcag gctcgcgccg ggcggcatcg cggtgctgtc ggggctgctc
      541 gcggatcagc aggccggcgt cgccgccgcc tatcgcgcgc aggggctgcg cctggtgcgc
      601 cgcgacctgc gccagggctg ggcgacgctc accgtcggcg cgcgctgacc agaaaccaag
      661 ggaggacgga ggacatggac gcgttcgatc tatccgggcg ggtggcgatc gtcaccggcg
      721 gcaatggcgg catcgggctc ggcatggcgg cggggctcgc gcgcgccggc gcgtcgctgc
      781 tgatcgccgg gcgcaacgag gacaagagcc gcgccgcggt ggccgagctc gaggcgctcg
      841 gcgccacggc ggcggcggtg cgggtcgaga tcaccgaccg tgcctcggtg caggcgatgg
      901 tcgccgccgc cggcgagcgc ttcgggcggc tcgacatcct ggtcaacaac gccggcacca
      961 acatccgcaa gcagcccgag gattacacgc ccgaggagtg gcacacggtg atcgagacca
     1021 acctcacctc ggcgttccac tgctgccagg cggcgtaccg gccgatggtg gcggcgggcg
     1081 gcggcaagat catcaacatc ggctcgatga tgtcgatctt cggcgcctcg ttcaccatgc
     1141 cctatgccag cagcaagggc ggcatcgtcc agatgaccaa ggcgctggcc tgcgcctggg
     1201 ccaaggacaa catccaggtc aatgcggtgc tgcccggctg gatcgacacc acgctcaccc
     1261 gccaggcgcg ccagcagatc gagggcctgc acgagcgcgt ggtcgcgcgc acgccggcgg
     1321 gccgttgggg catgccccgg gacttcgccg gcatcgcggt gttcctggcc gcgccggcgt
     1381 cggacttcct cactggtacc gccatcccgg tcgacggcgg ctactccgtc cagggttagt
     1441 ctggccggtt gatcgtatcg ggaattcgtt ggacggctga cgcgccggcg cccgtctcgg
     1501 ggcgccggcg cgcgggcgga ctcccaggtc agccgatcag ctggcgcaag cgcgctacca
     1561 ggttgtgcag ggcctggttg aacgcgcggg cgtgcagctc gcgcccgtgg gcgaccaatg
     1621 ccgcgatctc g
//
LOCUS       DASWHU010000152        15283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2660525, whole genome shotgun sequence.
ACCESSION   DASWHU010000152 DASWHU010000000
VERSION     DASWHU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15283
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660525"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VGB88_05820"
     CDS             <1..1482
                     /locus_tag="VGB88_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9646994.1"
                     /translation="PHADLKNIDPIWTTAYITRNHGYMVYDTLFALDEKLEVRPQMVE
                     RWEVSEDGLTWRFTLREGLLWHDGTAVTAADCVASLRRWGARDGMGQKLMDATEALWP
                     ADARTVVLELKAPYGLVLESLGKISSNVPFMMPERLARTDPFEQVPEIVGSGPFKFVA
                     GEWVPGAKVVYVRNPSYVPRAEPPSYAAGGKVAKVDRVEWHYIPDAATAMNALLAGEI
                     DYFELPPHDLLPIMASSPGVVVETLDPLGQQGWLRLNHTLPPFDNPKMRQAMLWMVSQ
                     AEYLQATVGNPEYYRTCPAYFGCGTALESGVGADALMTQDLDKARALVAEAGYDGTPV
                     VVMQATDIAINNGMALVTAQLLRQIGVHVELQAMDWSTLTSRRAMSDPVDQGGWNIFH
                     TWWTGADILNPVANIGVSGGCRERAWYGWPCDEEIERLRDAFAHATDPAEQKRLAEAV
                     QARAYRIVAYVPTGQWFQPTAYRDSLVGLIRSPVPFFWNVEKR"
     gene            1716..2657
                     /locus_tag="VGB88_05825"
     CDS             1716..2657
                     /locus_tag="VGB88_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9646995.1"
                     /translation="MLAFIARRVLATLPVMAVVAVFVFLLLHLSPGDPATVIAGDYAS
                     PADVARIRAQLGLDQPLHLQFVTWIGRVLQGDLGVSIFSNLPVSTLIAQRLEPTVALA
                     LLTLALAVAVAVPLGVIAAWQAGSWIDRLVMGFAVLGFSVPVFVLGYVLIYLLAIKLG
                     LFPVQGYAPLADGIWPFLHRLALPSVTLSVIFIALIARITRASVLEVLAEDYIRTAYA
                     KGLPERLVLLRHALRNAAVPIITVIGIGFSLLIGGVVVTESVYNIPGLGRLTVDAVLR
                     RDYPVIQGIILVFSAAYVVINLVIDILYTAFDPRIRY"
     gene            2729..3544
                     /locus_tag="VGB88_05830"
     CDS             2729..3544
                     /locus_tag="VGB88_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9646996.1"
                     /translation="MLRRHPGVMFGLAMIALMVAAAAAAPLLAGGDPAALNPIERLHA
                     PSAAHWLGTDMYGRDLYTRTVYGARISLVVGLCVTLLSVAFGLAIGLVSGFVRALDAV
                     VMRIMDGLMAIPGILLAIAMISLAGASLATVVIAITIPEIPRVVRLVRSVVLTIREEP
                     YVEAAITVGTRLPWLLLRHVLPNTIAPLIVQGTYVCASAIIVEALLSFLGAGIPPEIP
                     SWGNIMAEGRTFFQISPWIIFFPGLCLALTVLAINVLGDGLRDTLDPRIARRM"
     gene            3550..5187
                     /locus_tag="VGB88_05835"
     CDS             3550..5187
                     /locus_tag="VGB88_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9646997.1"
                     /translation="MADTPRAPVLEIRGLTVRLPPHADRANAIEDVSLALGEHETLCL
                     VGESGSGKSVTAQAIMGLLPKRQLVATAGEIRLQGEDLLRVSPARLRTLRGARMSMIF
                     QEPMTALNPVLPVGRQVEEVLSAHTTLPAPARARRVVEVMDAVNLPEPAALRHSYPHQ
                     LSGGQRQRVMIAMALALEPALLIADEPTTALDVTTQAQILRLIKSIQAERRTGVLFVT
                     HDFGVVAEIADRVAVMQHGRIVESGTTAQVLRAPEHPYTRMLIAAVPGLDPPARPAKA
                     AAPVALATRSLTKDYAGGGWFRKTRAVRAVDDVTIEVRRGETLGIVGESGSGKSTIAR
                     CLLRLVDPSAGEVLLHGAEIGHLSARALRAQRRRIQIVFQDPYRSLNPRRTIGAAIVE
                     GPINFGTPRAEAWARARDLLALVGIDARAAARYPHQFSGGQRQRICVARALAMEPEVL
                     VADEPVSALDVSVQAQVLKLLDDVRARFDLAMLFITHDLRVAAQVCDRIAVMYRGEVV
                     ETGPTRDLFANPRHDYTKRLFAAAPGRGWTFGAEPGA"
     gene            complement(5200..6564)
                     /locus_tag="VGB88_05840"
     CDS             complement(5200..6564)
                     /locus_tag="VGB88_05840"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9646998.1"
                     /translation="MQVCFALANRPEARRSLEVPERAVRLLAVLGKVTRGPCETELLP
                     DDLELARITAGEQAEDQTARLLQHTVDPPLDIDELNSIPPDAAVMGQCIDNAYAAADE
                     VGYSYAIVKSGQLAYMGQGGSARAPWEADFPNVAMTPLKPMTIASISKTITAVAVMKL
                     LEQNPSINLDDPFYPLIEDKFSGQVAFDGGLIATLPGPGVAAVSIRNLLMHRSGLTPG
                     LGCGYANLAKLIASGVVGTPGATYDYENSNFCLLREVIEQVSGMDYIDFVQDNVLGPM
                     GITDMACEPEDTDPTLYYNTIGDPGYLWGDFNATCSAYGWNASAVDLAQFMANVRVNT
                     VLTPESTEEMQNGSCLGGYCLGWIRSDNAIGVHHWHNGDWIDSDPCDVPGALDDIIFQ
                     PSTCPRGFNGTVMRFQLGIDAVLLVNTRGGTGIHPGLKSEVTILRECFAEAFLDANDI
                     ARVP"
     gene            complement(7049..8320)
                     /locus_tag="VGB88_05845"
     CDS             complement(7049..8320)
                     /locus_tag="VGB88_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEX9646999.1"
                     /translation="MDQLVLFERFAVALGIGILIGLERGWQERGRPEGSRVAGIRTFA
                     LIGMLGGVAALLSEHFGALVLAVLFAAFAVVMLVGRLRAVQETRDVGHTTTVAAMLTF
                     GLGALAVAGELALAAAGAVIAALLLSIKPALHAWLERIAYDELQAVLRLLVMSVVLLP
                     VLPNRGYGPWGALNPYELWLMVVLIAGISLVGYVIVRLAGARIGLLLAGLAGGLVSST
                     AVAIGFSRMAERQPAHAPALAAGIVAAAATMFPRTLLIAGALDRSLLAPLAWPLGAAA
                     LAGFAAALLLARGAAAEAGAGVGEIEHRNPFELGLALRFGALLAVIMVLARAVEATLG
                     DAGLYLVAAVSGMADVDAITLTYARLAGADIPAKAAVTGILIAAAVNTGIKGALASYI
                     GGRRLALAVAAPLAAALAAGAVAMWIGARIG"
     gene            complement(8322..9818)
                     /locus_tag="VGB88_05850"
     CDS             complement(8322..9818)
                     /locus_tag="VGB88_05850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9647000.1"
                     /translation="MSPTAAATPAPAAAGLAPRPLLAIALLALAARLAFVLAFPFGGG
                     DWDVYATVAENILRGCGVSLSDPAGPACVPHFGGNQGPGYPVFVALVWAASGHSDMAV
                     RLVQTALAALAIAYLADAAARAAARPAVGVAAGLVLAASPLAVAWPRYTQTETLALAA
                     TIWVLAELLRSLAAGRLRIAALGAALIAATFIRLDAILLAVPVAVAALALHGVRQGLA
                     RGAAVALVLALPWAAWTARNLAAGLPRLTPTDMVMPADAPSPLGYLAWTRTWITKEYQ
                     RPGALWPINRFVYSAISIDPTAYDGPGEQARIEALLAGLARHDGAPIPAAIDDAFRAI
                     AAERAARAPLRTWLANPLRRALALWSNPFSSFGWPNELPADAASHAERLQIAEGGLWA
                     KLALAKAYPWRAATKALTGGYRYLLLVAFLAAASAAALSRGMDAPVRRLAACAAGFVV
                     ARTLVLAWIGNIETRYTAALAPAMEVAVVLVAAASLAAWQRRPRPRGA"
     gene            complement(10079..11017)
                     /locus_tag="VGB88_05855"
     CDS             complement(10079..11017)
                     /locus_tag="VGB88_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9647001.1"
                     /translation="MQDEGAAKGTGRRAAWPLAALFCALVAGAPRAHGETFAPASDMI
                     GAPRLYVSDGSQPLMYVARAHDLGLLEVMAANPGVDPWVPGAGTPVMLPTAHLLPDAP
                     RKGLVINLAELRIYFFPADGGSVQSFPIGVGREAFTTPLGETKIVRKQVDPTWRPTEG
                     TRAAAPHIPAVVPPGPSNPLGDRALYLGWPTYAVHGTNKPWGVGRRVSRGCIRLYPED
                     IKALYELVPVGTPVTVVHQQVKLGWQDAELYLEVHPDHAQLDALEATGNFVPGLEADA
                     TPLILDTAGSEAERLDWDVIRAALKQERGLPVRITR"
     gene            complement(11052..11384)
                     /locus_tag="VGB88_05860"
     CDS             complement(11052..11384)
                     /locus_tag="VGB88_05860"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HEX9647002.1"
                     /translation="MPNMVLPKIAICAFVSLAALSGCATQEDLDALRQEVEQVKAQAA
                     GAQATAQQALQAAGAAQQTANAADQKATAAAADARQAAQDAARAREEAQAASEKADRI
                     FQESLRKM"
     gene            complement(11475..12029)
                     /locus_tag="VGB88_05865"
     CDS             complement(11475..12029)
                     /locus_tag="VGB88_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9647003.1"
                     /translation="MVKVAAMAAGHGRWLALGLALGLGAALAVALAAPPAEAEGATYL
                     RADRVLVLKSERRLQLLRGRTVLHEFRVSLGFDPVGHKLAEGDGRTPEGRYLLDGFNT
                     ESSFYRSIHISYPNGVDQLRAAHRGERTGGNIMIHGLPNGVDWKWDFRGGSDWTSGCI
                     AVSNEAMDVIWVSVSPGTPIDIRP"
     gene            complement(12023..12556)
                     /locus_tag="VGB88_05870"
     CDS             complement(12023..12556)
                     /locus_tag="VGB88_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX9647004.1"
                     /translation="MRIGIGRRAVIGGLAAAALLPGARAFAAKPAPRSLGFHHLHTGE
                     SLDACYWQDGAYLPDALAAIDHVLRDHRTGEASAIDRRLLDLLVAVRERLGSAAPFDV
                     ICGYRSPASNEMLRSTSSGVAKHSLHLAGRAIDVRLPGRELAALRAAGIDLARGGVGY
                     YPKSDFVHFDTGRVRRW"
     gene            complement(12630..13784)
                     /locus_tag="VGB88_05875"
     CDS             complement(12630..13784)
                     /locus_tag="VGB88_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9647005.1"
                     /translation="MLLAVALIVAAAPARALPPEIAPLEAALARYQALAAAGGWPAVG
                     AGPKLELGSVDARVPALRARLAATGDLARARAGAGPAFDAALDAAVRRFQARHGLLVD
                     GIVGPRTQAALDLPVGARIGQLARNLARLRARPPWPAGRFVLVHIAAFEAAAIDHGRE
                     ALRMRAIVGRPSRPTPELSARIDEVVFNPYWTVPPNIARLDIAPKVRADPGYLAARTI
                     RVFADWSYGAREVDPATVDWTKPLRVRLRQDPGPGNALGRVKFLFPNDYGVYLHDTPE
                     RRLFERPVRTFSSGCVRLARALELAEWLLEGEPGWTPAATAAELAAGRDRHVRLADPV
                     ALVIVYLTAWTDGDGTVQFRDDHYRRDLGEVAAAPQPCHIQPVDERGSAD"
     gene            13882..>15283
                     /gene="hpnR"
                     /locus_tag="VGB88_05880"
     CDS             13882..>15283
                     /gene="hpnR"
                     /locus_tag="VGB88_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HEX9647006.1"
                     /translation="MKMLAVHPGPLMYTKIYLRLEPLGLELIAEAARRDGHEVRLIDL
                     QVESHARYLALVRAWRPDVVAVSCNYLANVPEIVDLAKRTKALLPASYVVVGGHSASF
                     IAHELLEHGAGAIDCVLKGEGEPGIVALLRAVADDRGAAAEVPGAVTEAGCGPQPAFT
                     HSLDDVWPARDLLRHRRKYFIGVLDPCASIEFSRGCPWDCAFCSAWTFYGRSYRVMSP
                     ERVVADLERINEPGVFIVDDVAFIQERHGFEIGEAIAQKGIKKQYYLETRGDVLLRNK
                     EVFRFWRRLGIKYMFLGLEAIDEAGLKTYRKRVTLDENFAALEFARSLGIKVAVNIIA
                     DPDWDEHRFRVVREWCMEIPEIVNISINTPYPGTETWLTESRELVSRDYRLFDIQHAV
                     LPTRLPLATFYRELVETQKVLNAKHLGWSSLAATARIAAHHVLRGQTNFVRMLFKFNS
                     VYDPRLQLADHARPVAY"
BASE COUNT         2222 a         5486 c         5403 g         2172 t
ORIGIN      
        1 ccccacgccg acctcaagaa catcgacccg atctggacca ccgcctacat cacccgcaac
       61 cacggctaca tggtctacga caccctgttc gcgctcgacg aaaagctgga ggtccgcccg
      121 cagatggtcg agcgctggga ggtgagcgag gacggcctca cctggcgctt caccctgcgc
      181 gagggcctgt tgtggcacga cggcaccgcg gtcaccgccg ccgactgcgt cgcctcgctg
      241 cgccgctggg gggcgcgcga cggcatgggc cagaagctga tggacgcgac cgaggcgctg
      301 tggccggcgg atgcgcgcac cgtcgtgctc gagctcaagg cgccgtacgg gctggtgctc
      361 gagtccttgg gcaagatcag ctcgaacgtc cccttcatga tgcccgagcg cctcgcccgg
      421 accgacccgt tcgagcaggt gcccgagatc gtcggctcgg gcccgttcaa gttcgtcgcc
      481 ggcgagtggg tgccgggcgc caaggtggtc tatgtcagga accccagtta cgtgccgcgc
      541 gccgagccgc cgagctacgc ggcgggcggc aaggtggcca aggtcgaccg ggtcgagtgg
      601 cactacatcc cggacgcggc gaccgcgatg aacgcgctgc tcgcgggcga gatcgactat
      661 ttcgagctgc cgccgcacga cctgctgccg atcatggcgt cctcgcccgg ggtagtggtc
      721 gagaccctcg acccgctcgg ccagcaaggc tggctcaggc tcaaccacac cctgccgccg
      781 ttcgacaatc cgaagatgcg ccaggcgatg ctgtggatgg tgagccaggc cgagtacctg
      841 caggcgaccg tcggcaaccc cgagtactac cgtacctgcc cggcctattt cggctgcggc
      901 accgcgctcg agtcgggcgt cggcgcggac gcgctgatga cccaagatct cgacaaggcg
      961 cgcgcgctgg tcgccgaggc gggctacgac ggcacgccgg tggtggtcat gcaggcgacc
     1021 gacatcgcga tcaacaacgg catggcgctg gtcaccgccc agctgctgcg ccagatcggc
     1081 gtgcacgtcg agctgcaggc gatggactgg agcacgctga cctcgcggcg cgcgatgagc
     1141 gatccggtcg accagggcgg ctggaacatc ttccacacct ggtggaccgg cgccgacatc
     1201 ctcaacccgg tggccaatat cggcgtctcg ggcggttgcc gcgagcgcgc ctggtacggc
     1261 tggccgtgcg acgaggagat cgagcgcctg cgcgacgcct tcgcccacgc caccgacccg
     1321 gccgagcaga agcggctcgc cgaggcggtg caggcgcgcg cctaccggat cgtcgcctac
     1381 gtgccgaccg gccagtggtt ccagccgacc gcctatcgcg actccctcgt cgggctgatc
     1441 cgctcgccgg tccccttctt ctggaacgtc gagaagcggt agggaaacgg cggatcgccg
     1501 tgccgcagcc cgcccccctc ctgcctccct ccgcacgcgg gccgagagag tcacggattt
     1561 cagtccgcgc cgttcgttta tcgcccctca ccctgtccct ctcccggtgg gagagggaat
     1621 gcggcggcgg cgccgcgaca cacctctccc tggggagagg tcggccgcgc aagcggccgg
     1681 gtgaggggcg tcgtggtcgc gagtccggct gagcgatgct cgccttcatc gcgcggcgcg
     1741 tgctcgccac cttgccggtg atggcggtgg tggcggtgtt cgtgttcctg ctcttgcacc
     1801 tgagcccggg cgatccggcc accgtgatcg ccggcgacta cgcgagcccg gccgacgtcg
     1861 cccgcatccg cgcccagctc ggcctggatc agccgctcca ccttcagttc gtgacctgga
     1921 tcgggcgcgt gctccagggc gacctcggcg tctcgatctt ctccaacctg ccggtctcca
     1981 ccctgatcgc ccagcggctc gagcccacgg tcgcgctcgc gctcttgacg cttgcgctcg
     2041 cggtcgcggt cgcggtgccg ctcggcgtca tcgcggcgtg gcaggcgggc tcgtggatcg
     2101 accggctggt gatgggcttc gcggtgctcg gcttctcggt accggtgttc gtgctcggct
     2161 acgtactgat ctatctgctg gcgatcaagc tcggcctgtt cccggtccag ggctacgccc
     2221 cgctcgcgga cggcatctgg ccgttcctgc accgcctcgc gctgccgtcc gtcaccttga
     2281 gcgtcatctt tatcgccctg atcgcgcgca tcacgcgcgc gagcgtgctc gaggtgctgg
     2341 ccgaggacta catccgcacc gcctacgcca agggcttgcc cgaacggctg gtcctgctca
     2401 ggcacgcgct caggaacgcg gcggtgccga tcatcaccgt catcggcatc ggcttctcgc
     2461 tcctgatcgg cggcgtggtg gtgaccgaga gcgtctacaa catccccggc ctcggccggc
     2521 tcacggtcga tgcggtgctg cgccgcgact atcccgtcat ccagggcatc atcctggtgt
     2581 tctccgccgc ctatgtggtg atcaacctgg tgatcgacat cctgtatacc gcgttcgacc
     2641 cgaggatccg ctattgatca ccacggtcaa gagcgtcgag ctggcccagg aggcggcgct
     2701 cagcctcggg cgcgacagcg tctggcggct gcttcgccgc catccggggg tgatgttcgg
     2761 gctggcgatg atcgcgctga tggtcgccgc cgccgcggcg gcgccgctgc tcgcgggcgg
     2821 cgacccggcg gcgctcaacc cgatcgagcg gctgcatgcg ccctcggccg cgcactggct
     2881 gggtaccgac atgtacggcc gcgacctcta tacccgcacc gtctacggcg cgcgcatctc
     2941 gctggtggtc gggctgtgcg tgacgctgtt gagcgtcgcc ttcgggcttg ccatcggcct
     3001 ggtctcgggc ttcgtgcgcg cgctcgatgc ggtggtgatg cggatcatgg acgggctgat
     3061 ggcgattccc ggcatcctgc tcgcgatcgc catgatctcg cttgccggcg cgagcctggc
     3121 gacggtggtg atcgcgatca ccatccccga gatcccgcgc gtggtccgcc tggtgcggag
     3181 cgtcgtgctg accatccgcg aggagcccta tgtcgaggcg gcgatcacgg tcggcacgcg
     3241 cctgccctgg ctgctgctcc gccacgtgct gcccaacacc atcgcgccgc tgatcgtcca
     3301 gggcacctat gtctgtgcat cggcaatcat cgtcgaggca ttgttgagct tcctcggcgc
     3361 cggcatcccg cccgagatcc cgagctgggg caatatcatg gccgagggcc gcaccttctt
     3421 ccagatctcg ccgtggatca tcttcttccc cggcctgtgc ctggcgctga cggtgctggc
     3481 gatcaacgtg ctcggcgacg ggttgcgcga cacgctcgac ccgcgcatcg cgcgccggat
     3541 gtgagcgccg tggcggacac gccccgcgcg ccggtgctcg agattcgcgg cctcaccgtg
     3601 cgcctgccgc cccatgccga ccgcgcaaac gcgatcgagg acgtgagcct cgccctgggc
     3661 gagcacgaga ccttgtgcct ggtcggcgag tcgggctcgg gcaagtcggt caccgcccag
     3721 gcgatcatgg ggctgttgcc caagcgccag ctggtggcga ccgcgggcga gatccgcctc
     3781 cagggcgagg acctgctgcg cgtgagcccg gcgcggctgc gcaccttgcg cggcgcgcgc
     3841 atgtcgatga tcttccagga gccgatgacc gcgctcaacc cggtcctccc cgtaggccgc
     3901 caggtcgagg aggtgctgag cgcgcacacg acattgccgg cgccggcgcg cgcgcgccgc
     3961 gtggtcgagg tgatggacgc ggtcaacctg cccgagccgg cggcgttgcg ccacagctac
     4021 ccgcaccagc tgtcgggcgg ccagcgccag cgggtgatga tcgcgatggc gctcgcgctc
     4081 gagcccgcgc tcctgatcgc cgacgagccg accaccgcgc tcgacgtcac cacccaggcg
     4141 cagatcctga gattgatcaa gtcgatccag gcggagcgcc gcaccggggt gctgttcgtc
     4201 acccacgact tcggcgtcgt cgccgagatc gccgaccggg tggcggtgat gcagcacggg
     4261 cgcatcgtcg aatccggcac cacggcgcag gtgctgcgcg cgcccgagca cccctatacg
     4321 cgcatgctga tcgcggccgt gcccgggctc gacccgcccg cgcgcccggc caaggccgcg
     4381 gcgccggtcg cgctcgccac ccgctcactg accaaggact atgccggcgg cggctggttc
     4441 cgcaagacgc gcgcggtgcg cgcggtcgac gacgtgacga tcgaggttcg ccgcggcgag
     4501 accctcggca tcgtcggcga gtcgggctcg ggcaagtcga cgatcgcgcg ctgcctgttg
     4561 cgcctggtcg acccgagcgc gggcgaggtg ctgctgcacg gcgccgagat cggccacctg
     4621 tccgcacgcg cgctgcgcgc ccagcgccgg cgcatccaga tcgtgttcca ggacccctac
     4681 cggtcgctga atccgcgccg gaccatcggc gcggcgatcg tcgaggggcc gatcaacttc
     4741 ggcaccccgc gcgccgaggc ctgggcgcgc gcgcgcgatc tcctcgccct ggtcggcatc
     4801 gacgcgcgcg ccgcggcgcg ctatccgcac cagttctcgg gcggccagcg ccagcgcatc
     4861 tgcgtcgcgc gtgcgctcgc catggagccc gaggtgctgg tcgccgacga gccggtctcg
     4921 gcgctcgacg tctcggtcca ggcgcaggtg ctcaagctcc tggacgatgt gcgcgcgcgc
     4981 ttcgacctcg ccatgctgtt catcacccac gacctgcgcg tcgccgccca ggtgtgcgac
     5041 cggatcgcgg tcatgtaccg gggcgaggtg gtcgagaccg gcccgacccg cgacctgttc
     5101 gcaaacccgc gccacgacta caccaagcgc ctgttcgccg ccgcccccgg ccgcggctgg
     5161 acattcggcg ccgagcccgg cgcctgagcg ccggcgtcct cagggcacgc gcgctatgtc
     5221 gttggcatcg aggaatgcct cggcgaagca ttcgcgcagg atggtgacct cgctcttgag
     5281 gcccggatgg attccagtgc cgccgcgcgt attgaccagc aacacggcgt cgatgccgag
     5341 ctggaaccgc atgaccgtgc cgttgaagcc gcgcgggcag gtcgagggct ggaagatgat
     5401 gtcgtcgagc gcgccgggca cgtcgcacgg gtcggaatct atccagtcgc cgttgtgcca
     5461 atgatggacg ccgatcgcgt tatcggaccg aatccatccc aggcagtagc cgccgaggca
     5521 ggacccgttc tgcatctcct cggtcgactc cggtgtcaga acggtgttga cacggacgtt
     5581 ggccatgaac tgggcgagat cgacggccga cgcgttccag ccgtaggcgc tacacgtcgc
     5641 attgaaatcg ccccagagat aacccggatc gccgatcgtg ttgtaataga gcgtcgggtc
     5701 ggtgtcctcg ggctcacacg ccatgtcggt gatgcccatc ggaccgagca cgttgtcctg
     5761 gacgaagtcg atgtagtcca tgccgctgac ctgctcgatt acctcgcgca gcaggcagaa
     5821 gttcgagttt tcgtagtcgt aggtcgcgcc cggcgtgccg accacgcccg acgcgatcag
     5881 cttggcgaga ttggcatagc cgcagccgag gccgggcgtc agcccgctgc ggtgcatcag
     5941 caggttgcga atcgaaaccg ccgcgacgcc cggtcccggc aaggtcgcga tcaggccgcc
     6001 gtcgaacgcg acctggccac tgaacttgtc ctcgatcaac ggatagaacg ggtcgtccaa
     6061 gttgatggac ggattctgtt cgagcagctt catcaccgcc accgccgtga tggtcttcga
     6121 gatgctggcg atcgtcatcg gcttgagcgg cgtcatggcg acgttcggga agtccgcctc
     6181 ccacggcgcg cgcgccgagc cgccctggcc catgtatgcc agctgcccgg acttgacgat
     6241 cgcgtaggaa tagccgacct cgtccgccgc tgcataggcg ttgtcgatac actggcccat
     6301 gaccgcggca tccggcggga ttgagttgag ctcgtcaatg tcgagcggtg ggtcgaccgt
     6361 gtgctgcagc agccgggcgg tctggtcctc agcctgctcg ccggcggtga tccgcgccag
     6421 ctcgaggtcg tcgggcaaga gctccgtctc gcacggcccg cgcgtgacct tgccgagcac
     6481 cgcgagcagg cgtacggcgc gttcgggaac ctcgagcgag cggcgcgcct cgggtcgatt
     6541 agccagggcg aaacagacct gcacgagccc gccgcggtgg gccgcaaacg gttgcgcaag
     6601 ggcattgccg gacgaggccg taaccagcga aacgcatgcg acgaggaggg tcgaacggag
     6661 tagccgcgcc atcgttttcc ctttcctggc ctgatcgaga ggaccaccat cgagctcccc
     6721 aacgcgccgg cgagattgag cgccaattcg cggcgggccc cgctcagacc gtggtaggca
     6781 tcaaggtcgg cctaaacgcg ctcaagcaca gcgacgatcc ggctttttcc ggtcctcccc
     6841 gggcgaccat ttccggacaa caagaatctt accataactc tgccgcactg gcgatgaagc
     6901 cttggtcggc tggccgaaca acgttcgacg tcgtcgtaca cgttgagggt cactgcggaa
     6961 atgactgcac ggcggaagga tgatctgcgc aaggtcaggt acgggagatc ccggcgctgc
     7021 gtggcagagc cgggtcagca atacgcgact aaccgatgcg tgccccgatc cacatcgcga
     7081 ccgcgccggc ggcgagcgcg gcggcgagcg gggcggcgac cgcgagcgcg aggcgccggc
     7141 cgccgatgta ggacgcgagc gcgcccttga tgccggtatt gaccgccgcc gcgatcagga
     7201 tgccggtcac cgccgccttg gccgggatgt cggcgccggc gagccgggca taggtaaggg
     7261 tgatcgcatc cacgtcggcc atgcccgaga ccgcggcgac cagatacagc ccggcatcgc
     7321 cgagcgtcgc ctcgacggcg cgcgcgagca ccatgatcac ggccaggagc gcgccgaacc
     7381 tgagcgccag gccgagctcg aacgggttgc ggtgctcgat ctcgccgacc ccggcgccgg
     7441 cctccgccgc ggcgccccgc gcgagcagca gggcggcggc gaagcccgcc aaagccgccg
     7501 cgccgagcgg ccaagcgagg ggggcgagca ggctccggtc gagcgcgccc gcgatcagga
     7561 gggtgcgcgg gaacatggtc gcggcggcgg cgacgatgcc ggcggcgagc gccggcgcgt
     7621 gcgcgggctg acgctcggcc atgcgcgaga agccgatcgc gaccgcggtc gaggacacca
     7681 ggccgccggc gagcccggcc agcaagaggc cgatgcgcgc gcccgccagc cgcacgatca
     7741 catagccgac caacgatatg ccggcgatca ggaccaccat cagccacagc tcgtaagggt
     7801 tcagcgcgcc ccacggcccg tagccccggt tcggcagcac cggcagcagc accaccgaca
     7861 tgaccagcaa tcgcaacacc gcctgcagct cgtcataggc gatgcgctcg agccaggcgt
     7921 gcagcgccgg cttgatgctg agcagcagcg ccgcgatcac cgctccggcg gcggcgagcg
     7981 cgagctcgcc cgcgaccgcc agcgcgccga gcccgaaggt gagcatcgcc gcgaccgtgg
     8041 tggtgtggcc gacgtcgcgg gtctcctgga ccgcgcgcag ccgcccgacc agcatcacca
     8101 ccgcgaaggc ggcgaacagg accgcgagca ccagcgcgcc gaagtgctcg gacaagagcg
     8161 ccgcgacccc gcccagcatg ccgatcaggg cgaaggtgcg gatgccggcg acgcgcgagc
     8221 cctcgggccg gccgcgctcc tgccagccgc gctcgagccc gatcaggatg ccgatgccga
     8281 gcgcgaccgc gaaccgctcg aacagcacca gctggtccac ctcaggcgcc cctcggtcgc
     8341 gggcgccgct gccacgccgc caggctcgcg gccgcgacca gcaccaccgc gacctccatc
     8401 gccggcgcga gcgcggcggt gtagcgggtc tcgatgttgc cgatccacgc caacacgagc
     8461 gtgcgcgcga cgacgaagcc ggcggcacag gcggcgagcc gccgcaccgg cgcgtccatc
     8521 ccgcgcgaga gcgcggcggc cgaggccgca gcaaggaacg cgaccagcag caggtagcgg
     8581 tagccgccgg tcagcgcctt ggtcgccgcc cgccacgggt aggccttggc cagcgccagc
     8641 ttggcccaga ggccgccctc ggcgatctga aggcgctcgg cgtggctcgc cgcgtccgcg
     8701 ggcagctcgt tcggccagcc gaagctcgag aacgggttcg accacaaggc gagcgcgcgc
     8761 ctgagcgggt tggcgagcca ggtgcgcagc ggcgcgcgcg ccgcccgctc ggccgcaatc
     8821 gcgcggaacg catcgtcgat cgcggccggg atcggcgcgc cgtcgtggcg ggcgagcccg
     8881 gcgagcagcg cctcgattcg cgcctgctcc cccggcccgt cataggccgt gggatcgatg
     8941 ctgatggccg agtagacgaa ccggttgatc ggccacagcg cgcccggccg ctggtactcc
     9001 ttggtgatcc aggtgcgcgt ccaggcgagg tagccgagcg gcgagggcgc atcggcgggc
     9061 atgaccatgt cggtcggcgt gagccgcggc agcccggccg cgaggttgcg cgcggtccag
     9121 gcggcccagg gcagcgccag gaccagggcg accgcggcgc cgcgcgccag gccctggcgc
     9181 acgccgtgca gcgccaacgc ggcgacggcg accggcaccg cgagcaagat ggcgtcgagg
     9241 cggatgaagg tcgccgcgat cagcgccgct cccagggccg cgatgcgcag ccgtccggcg
     9301 gcgagcgagc gcagcagctc ggcgagcacc cagatcgtcg ccgccagcgc caaggtctcg
     9361 gtctgggtat agcgcggcca ggcgacggcg agcggcgagg ccgccagcac cagcccggcg
     9421 gcgacgccga ccgcgggccg tgccgccgcg cgcgcagccg catcggccag ataagcgatg
     9481 gcaagcgcgg cgagcgcggt ctggaccagg cgcaccgcca tgtccgagtg gccggaggcg
     9541 gcccacacca gcgccacgaa caccgggtag ccggggccct gattgccgcc gaaatggggc
     9601 acgcaggcgg gccctgccgg gtccgacagc gacaccccgc agccgcgcaa gatgttctcg
     9661 gccacggtcg cgtagacgtc ccagtcgccg ccgccgaacg ggaaggcgag cacgaacgcg
     9721 agccgcgcgg cgagggcgag cagggcgatg gcgagcagcg gccgcggcgc gaggccggcc
     9781 gcggccggcg ccggcgtcgc tgcggcggtc gggctcatca ccccactcta ttgccgccgc
     9841 cgcccggcac aagggcgcca cgcggccggc ggggctagag cgggatgata ttaagtcgga
     9901 tccctgagcc tgttttggtc gtcaccccgg actccgcgaa gcggatatcc ggggtccata
     9961 cgcccttgcc acgcactcga ccacctgtgc aagcggcgcg atggaccccg gctcgcggcg
    10021 ctgcgcgcct tggccggggt gacgggcgat ggatgaccat gtgtcaaatc atcaggcgct
    10081 agcgggtgat gcgcaccggc aggccgcgct cctgcttgag ggcggcgcgg atcacatccc
    10141 agtccagccg ttcggcctcg ctgccggcgg tgtccaggat cagcggcgtg gcgtcggcct
    10201 cgaggccggg cacgaagttg ccggtggcct ccagcgcatc gagctgggcg tggtcgggat
    10261 ggacctcgag atagagctcg gcgtcctgcc agccgagctt gacctgctgg tggaccacgg
    10321 tgaccggcgt gccgaccggc accagctcgt acagcgcctt gatgtcttcc gggtacagcc
    10381 ggatgcagcc ccggctgacc ctgcgcccga ccccccacgg cttgttggtg ccgtggaccg
    10441 cataagtcgg ccagcccaga tagagcgccc ggtcgccgag cgggttgctc ggccccggcg
    10501 gcaccaccgc cgggatatgc ggcgcggccg cgcgcgtccc ctcggtcggg cgccaggtcg
    10561 ggtcgacctg cttgcgcacg atcttggtct cgccgagcgg cgtggtgaag gcctcgcgcc
    10621 cgaccccgat cgggaagctc tgcaccgacc cgccgtcggc cgggaagaag tagatgcgca
    10681 gctccgccag gttgatcacc aggcccttgc gcggcgcatc gggcaacagg tgcgcggtcg
    10741 gcagcatcac cggcgtgccc gctccgggca cccacgggtc gacgcccgga ttcgccgcca
    10801 tcacctcgag caggccgagg tcgtgggcgc gcgccacgta catcagcggc tggctgccgt
    10861 cgctgacata gaggcgcggc gcgccgatca tgtcggaggc gggcgcgaag gtctcgccat
    10921 gggcgcgcgg cgcgcccgcc accagggcgc aaaacagcgc ggcgagcggc catgccgctc
    10981 gccggcccgt gcccttggcc gcgccctcgt cctgcatcgg gccgcggccg atcgcccggg
    11041 gttccgggtc gctacatctt gcgcagcgac tcttggaaga tcctgtcggc cttttcgctt
    11101 gcagcctgag cctcctcgcg cgcgcgcgcc gcatcctgcg ccgcctgacg cgcgtcggcg
    11161 gcggcggcag tggccttctg gtcggccgcg ttggcggtct gctgggccgc gcccgcggcc
    11221 tgcagtgcct gctgcgcggt ggcctgggcg cccgccgcct gtgccttgac ctgctcgacc
    11281 tcctggcgca gcgcgtccag gtcctcctgg gtcgcgcaac ccgacaaggc ggccaacgag
    11341 acgaacgcgc agatcgcgat cttgggaagt accatgttcg gcatgtctac cgaccctccg
    11401 gttcgaagcc aatcatcagt tcgatgaacg gtcgccgtcc gggcttgtgc cctgccccgg
    11461 ccggtgcccc gccgctacgg acgaatgtcg atgggcgtgc ccgggctcac cgagacccag
    11521 atcacgtcca tcgcctcatt gctaaccgca atacaaccgg acgtccagtc cgatccgccc
    11581 cggaaatccc acttccagtc gacgccgttg ggcaggccgt ggatcatgat gttgccgccg
    11641 gtgcgctcgc cgcggtgcgc cgcccgcaac tggtcgacgc cgttgggata ggagatgtgg
    11701 atcgagcggt agaacgagct ttcggtgttg aagccgtcga gcaggtaacg tccctcgggc
    11761 gtgcgcccgt cgccttcagc gagcttgtgc ccgaccgggt cgaagccgag cgagacgcgg
    11821 aactcgtgca gcacggtgcg cccgcgcaac agctgcaggc gccgctcgga cttgagcacc
    11881 agcacccggt cggcgcgcag ataggtcgcg ccctcggcct cggccggcgg cgcggccaac
    11941 gccacggcca gcgccgcccc gaggcccagc gcgaggccca gcgcgagcca gcggccatgt
    12001 cccgccgcca tcgccgcaac cttcaccacc gccgcacgcg cccggtatcg aagtggacga
    12061 aatccgactt cgggtagtag ccgacgccgc cgcgggcgag atcgatcccg gccgcgcgca
    12121 gcgcggccag ctcgcgtccc ggcaggcgca cgtcgatcgc ccggccggcc aggtgcaggc
    12181 tgtgcttggc gacgccgctc gaggtgctgc gcagcatctc gttgctcgcc ggcgagcgat
    12241 agccgcagat cacgtcgaac ggcgcggcgc tgcccaggcg ctcgcgcacc gcgaccagca
    12301 ggtcgagcag cctgcggtcg atggcgctgg cctcgccggt gcggtggtcg cgcagcacgt
    12361 ggtcgatcgc ggccagcgcg tccggcaggt aggcgccgtc ctgccagtag caggcatcga
    12421 gcgattcgcc ggtgtgcaga tggtggaagc cgaggcttct gggggcgggc ttggcggcga
    12481 aggcgcgcgc gccgggcagc agcgcggcgg cggcgaggcc gccgatcacg gccctgcgcc
    12541 cgatcccgat ccgcatcccc gcgttcggca tgcgtccccc accgcttggt cccgacgccc
    12601 acgatcgccg ccgattcgta accgaactcc taatcggcgg acccgcgctc gtcaaccggt
    12661 tgaatatggc atggctgcgg ggcggcggcc acttcgccca ggtcacggcg gtagtggtcg
    12721 tcacggaact ggaccgtgcc gtcgccgtcg gtccaggcgg tgagatagac gatcaccagc
    12781 gccaccgggt cggcgaggcg cacatgccgg tcccggcccg cagcgagctc cgccgcggtc
    12841 gcggccggcg tccagccggg ctcgccctcg agcagccatt cggcgagctc gagcgcacgc
    12901 gcaaggcgca cgcagcccga gctgaacgtg cgcaccggac gctcgaacag ccggcgctcc
    12961 ggagtgtcgt gcaggtagac gccatagtcg ttcggaaaca ggaacttgac ccggccgagg
    13021 gcgttgccgg ggccggggtc ctggcgcagc cgcacgcgca acggcttggt ccagtcgacc
    13081 gtcgccgggt cgacctcgcg cgcaccgtag ctccagtcgg cgaacacccg gatggtgcgc
    13141 gccgccagat agccggggtc ggcgcgcacc ttgggcgcga tgtcgaggcg agcgatgttc
    13201 ggcggcactg tccagtaggg gttgaacacc acctcgtcga tgcgcgcgct gagctcgggc
    13261 gtcggacggc tcggccggcc gacgatcgcg cgcatgcgca gcgcctcccg gccgtggtcg
    13321 atcgccgccg cctcgaacgc ggcgatgtgg accagcacga agcgccccgc cggccacggc
    13381 gggcgggcgc ggagccgggc gaggttgcgc gcaagctggc cgatgcgcgc gccgacgggc
    13441 aggtcgagcg cggcctgggt gcgcggcccg acgatgccgt cgacgagcag gccgtggcgc
    13501 gcctggaagc ggcgcaccgc ggcatcgagc gcggcatcga aggcgggccc ggcgccggcg
    13561 cgcgcacgcg cgaggtcgcc ggtcgcggcc aggcgcgcgc gcaaggccgg gacgcgcgca
    13621 tcgacgctgc cgagctcgag cttcgggcct gcgccgaccg ccggccagcc gcccgccgcg
    13681 gcgagcgcct ggtagcgggc gagcgcggcc tcgagcggtg cgatctcggg cggcagggcg
    13741 cgcgccggcg ccgccgcgac gatcagcgcg acggcaagca gcacgccggc agcgcgggtt
    13801 cgtgcccggc gcacccctgt gctaggcttg tcggccggcg cgcgacgccg tgccgcgaag
    13861 cgtgagcggg ggatcgaagt catgaagatg ctggcggtcc accccgggcc gttgatgtac
    13921 accaagatct acctccggct cgagccgctc gggctcgagc tgatcgccga ggccgcgcgc
    13981 cgcgacggcc acgaggtgcg cctgatcgac ctgcaggtcg agagccatgc ccgctacctg
    14041 gcgctggtcc gggcgtggcg gcccgatgtc gtcgcggtct cgtgcaacta tctcgccaac
    14101 gtgcccgaga tcgtcgatct cgccaagcgt accaaggccc tgctgccggc gagctacgtg
    14161 gtggtcggcg gccacagcgc ctcgttcatc gcgcatgagc tgctggagca cggcgcgggc
    14221 gcgatcgact gcgtgctcaa gggcgagggc gagcccggca tcgttgccct gctgcgcgcg
    14281 gtcgcggacg accgcggcgc ggccgctgag gtgccgggcg cggtcaccga ggcgggatgc
    14341 ggcccgcagc ccgccttcac ccacagcctc gacgacgtct ggccggcgcg cgacttgctc
    14401 cgccatcgcc gcaagtactt catcggtgtg ctcgacccgt gcgcctcgat cgagttctcg
    14461 cgcggctgcc cgtgggactg cgcgttctgc agcgcgtgga cgttctacgg ccgcagctat
    14521 cgggtgatga gcccggaacg cgtggtcgcc gatctcgagc gcatcaatga gcccggcgtg
    14581 ttcatcgtcg acgacgtcgc cttcatccag gagcggcacg gcttcgagat cggcgaggcg
    14641 atcgcgcaga agggcatcaa gaagcagtac tacctggaga cgcgcggcga cgtgctgctg
    14701 cgcaacaagg aggtgttccg gttctggcgc aggctcggca tcaagtacat gttcctgggc
    14761 ctcgaggcga tcgacgaggc cgggctcaag acataccgca agcgcgtgac cttggacgag
    14821 aacttcgcgg cgctcgagtt cgcgcgctcg ctcggcatca aggtcgcggt caacatcatt
    14881 gccgatcccg actgggacga gcatcgcttc cgggtggtgc gcgagtggtg catggagatc
    14941 cctgagatcg tcaacatcag catcaacacg ccctatcccg ggaccgagac ctggctgacc
    15001 gagtcgcgcg agctcgtctc gcgcgactac cggctgttcg acatccagca cgcggtgctg
    15061 ccgacccggc tgccgctggc gaccttctac cgcgagctgg tcgagaccca gaaggtgctg
    15121 aatgcgaagc atctcggctg gtcgtcgctg gctgcgaccg cgcgcatcgc cgcgcatcac
    15181 gtgctgcgcg gccagaccaa cttcgtgcgc atgctgttca agttcaacag cgtctacgac
    15241 ccgcgtttgc agctcgccga ccacgcccgc ccggtcgcct acc
//
LOCUS       DASWHU010000153        10390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3036437, whole genome shotgun sequence.
ACCESSION   DASWHU010000153 DASWHU010000000
VERSION     DASWHU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036437"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VGB88_05885"
     CDS             <1..1197
                     /locus_tag="VGB88_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9647007.1"
                     /translation="GEIGLSQLARNAVAALTSFTLSPIPLFLLMGEILFHSGVALKAI
                     DAVERLIARVPGRLSVVSIVGGTVFASLSGSTIANTAVLGNLLLPDMLRRGYHPSIAM
                     GPIMATGGIAMLIPPSALAVLLGSLAGISISGLLIAGIVPGVIMSLLFVGYVIGRCAL
                     DRSLAPAGDDAPRTEGGRWRPFLIYVLPLFGIFAVVVGSIFAGWATPTESAALGCVAS
                     AAAAAGYRALTWRGLMTAVRETAKISTMILFIIVASVTYAQILAFSGATDGLLRLIEG
                     FGLGPLLLLVVMLLVLLMLGCFMDQVSMVLITLPFFIPLAQANHIDLLWLGVLMLIAM
                     EISFTTPPFGLLVFVMKGVAPPTITIRQVYAAALPFIGLEALVLAMLVAWPKLAVWLP
                     SAILNR"
     gene            complement(1292..3829)
                     /locus_tag="VGB88_05890"
     CDS             complement(1292..3829)
                     /locus_tag="VGB88_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9647008.1"
                     /translation="MIGAKVLRHPILVLAAALVGLPHLVPALGLTTSVATEIALFALV
                     GLGFNLLLGYSGLVSFGHGAFFGLAAYAAALLQIHVLPGALLVPLAAAVLFSALLGLV
                     IGFLVLRRRGVYFSLLTLAFTAMFFYVVYRWTSFTGGENGLGGIARPDLWGIDIDDQT
                     TFYYLVAAIVLLVAWLIWRVVQSPFGCALQAIRDNEQRASFVGYPVRRYKLIAFVLSA
                     SILGLAGSLFAFLKYFVSADLVHVMFSGEILAMSIIGGMGAFLGPALGAGFFILFREI
                     LSDYTSAWQFYFGLLFMAFILFSPKGLVGLGTRLLAPLTRHEVRAAAMAARAVPEMRA
                     EIPRFLVAGPARSERAVVFACRDVTKRFGRFRAVAGVDLELRDRELRALIGPNGAGKT
                     TLFNLISGMYPPDEGELALFGSAVAGLPADRLARLGLARSFQITNLFPTLSVHENLRL
                     GVQARHKGRLDLWRNAGSFARVHEETRALIGLLGFEGIEHVAVSDLSYGMQRLLEIGL
                     ALAARPRVLLLDEPLAGLADQERERIVGLIRALARHMSVTLVEHDIDRVFAFAEVITV
                     MNAGLVLAEGSPDEVRAHPEVQQVYLGSGRQVVTARRAAPSRASGESILRLDNVNLFY
                     GKSHILHDVSLDVRKGEVVALLGRNGAGKSSTVKGIMGIVPPRTGRIVFDGRAIEGSA
                     PEDVARLGIGLVPQGRRLFANLTVADNLALGRLRRTGGDGARWSREEMFARFPRIEER
                     FAAKADVLSGGEQQMVAIARALSGNVRLLLLDEPFEGLSPTMVEEVFAAIDRLRQDVS
                     MLIIEHDLDLVLALADRAYVLDRGAITHEGPAALLTDIAYRKEKLWV"
     gene            complement(3826..4695)
                     /locus_tag="VGB88_05895"
     CDS             complement(3826..4695)
                     /locus_tag="VGB88_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9647009.1"
                     /translation="MLIVLEHTLNGLLIGAYYVMLALGLSLIFSLGGVVNLAHGAFYA
                     LGAYLAVTVSPMLGFGGALIASPVAVAALGMAIEHVLLRRLYREDPTLSLLFTFGLAL
                     TAEQALRLVWGATGLPFAIPPALRGQLLVGDFIYSYYRLAILGVAVAAVGGTWLLLTR
                     TAFGMIVRAGVHDPEMVRALGIRLRPVLAAIFGLGVGLAALAGVVSAPLAGVQPAMGT
                     EILTAAFVTVVIGGLGSFWGVVFGGLLIGVVRGMTVLFYPPAAEASMYALMVLVLLVR
                     PRGLAGESVGRLE"
     gene            complement(4817..6100)
                     /locus_tag="VGB88_05900"
     CDS             complement(4817..6100)
                     /locus_tag="VGB88_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647010.1"
                     /translation="MPSNTKIGRRGGKVLSRRAVLRGAALGAGGAVLGQLGFPAVLKA
                     EDTIRVGHLTPRTGFLGQLGEYGFRGAAMAVDEVNAAGGVLGRPLELISEDSVNPGVA
                     VTKTQKLVERDQVQFLFGEISSASGLAIAEQANRYKIIYVNTGCNSDALRGSNCNRFT
                     FHVEGCNTMYTKTIGQWQVRQNLITGAKWYMLTADYAFGHDLYGVSSRFLQQNGGVLV
                     NNDMVATNTPDYSGYILKIREAQPDFVYINLAGVDQTTFLKQYREYGLPYPVAGGVMD
                     TVPFWAAGIESLSGYWQSLWYHGLDTAASQAFTTAYTDRHGMPPDNQAWGDHIAIKIF
                     AQAVNEVGSADTAALIEHFEKGASFDLLKSRAGSFRDWDHQLLQEMYVVRVKDPAAAR
                     DKWDIFDVVEAVPSADQPLDLIQPTRDENPCTFAG"
     gene            6361..6627
                     /locus_tag="VGB88_05905"
     CDS             6361..6627
                     /locus_tag="VGB88_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9647011.1"
                     /translation="MIVATQMRAARALLGIDQRRLAELSGLSVPTIQRMEASEGMVRG
                     NVDSLMKVVSALEAAGVELIGDGAASQDGGRGVRLKAPPAGASA"
     gene            6684..8696
                     /gene="hyfB"
                     /locus_tag="VGB88_05910"
     CDS             6684..8696
                     /gene="hyfB"
                     /locus_tag="VGB88_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX9647012.1"
                     /translation="MTIAVLLWCVAVLLGTALAALAAGRSPAGTAVVYGASLAASLAA
                     LLAALAALIAGEATAPAVTLPLGLPWIGSHFRLDALAAFFLVVVNLGGAAASLYALGH
                     GRHEPEPARVLPFYPAFLAAMNLVVLADDAFTFLFSWELMSLASWALVMAHHRDADNA
                     RAGYVYLVMASFGTLALLLCVGLLAGPDGGYAFAAIRAGAHRPWVAAAVLALALLGAG
                     SKAGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRIVFDLLGEPGWWQSMVV
                     LALGGITAVLGVLHALMQHDLKRLLAYHTVENIGIIFIGLGLALAFQANGMGWAAALA
                     LTAALFHVFNHSLFKSLLFFGAGGVLAATRERDMEHLGGLIHRMPYTSFAFLVGCVAI
                     SALPPLNGFVSEWLTFQAILQSPELPQWGLKIMVPAVGGMLALSAALAAACFVKAFGV
                     TFLGRPRTAAAAAAAEVDRFSLAAMFVLAGLCLLAGILPGIVIDALGPVSRSLLGTRM
                     MPQSGLPWLSIVPIAESRSSYNGLLVFAFIAASASVAVYAIHRWASRALRRAPAWDCG
                     FPDPSPATQYTAGSFAQPIRRVFGTLVFRAREQVEMPPPGDARPAHLTVTLRDLIWDG
                     VYAPAADAVGFAAERLNHFQFLTIRRYVSLVFLALVTLLLVLALWR"
     gene            8687..9643
                     /locus_tag="VGB88_05915"
     CDS             8687..9643
                     /locus_tag="VGB88_05915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9647013.1"
                     /translation="MAVILDLLVQGAQMALVLLLAPLLTGLVRKVKARLLRRRGPPLI
                     QPYRDLLKLLRKEVVLADSASWLFRITPYLIFATTWVAAALVPTFATGLMFSWTADLI
                     AIIALLASARFFLALAGMDVGTSFGGIGSSREVMIASLAEPAMLLIVFTLALVAGSTQ
                     LSTVAAVMASPEVGLRVSLGLALIALIMVAIAENGRIPVDNPATHLELTMVHEAMVLE
                     YSGRHLAMIEFAASLKLLLYVSLISCLFAPWGLAIADGGTLGYLIGGAAYLAKLAAGG
                     IVLALFETAIAKMRVFRVPEFLGAALMLGLLATLLLFVSRSL"
     gene            9644..10306
                     /locus_tag="VGB88_05920"
     CDS             9644..10306
                     /locus_tag="VGB88_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component E"
                     /protein_id="HEX9647014.1"
                     /translation="MDRLVFDIAHLLAGGLVLVSFLLLYQDRLYALLNIYALHALVLA
                     MSVAWQAFAQNADHLYVTAAIALAFKAILVPGALHRIIARLGIHREIETVVGIGPTML
                     AGIGLVALSMVVMLRVTMAADPLAREDLAFALSVVLLGLLMMVTRRNAVGQVVGFMSL
                     ENGLVLAATGAKGMPLVVEISVAFSVLIALIVIGIFLFRIRERFDTVDVQVIDRHRGE
                     RP"
BASE COUNT         1490 a         3650 c         3565 g         1685 t
ORIGIN      
        1 ggcgagatcg gcctcagcca gctcgcgcgc aacgcggtgg cggcgctgac cagcttcaca
       61 ttgtcgccga tcccgctgtt tttgctgatg ggcgagatcc tgttccacag cggcgtcgcg
      121 ctcaaggcga tcgatgccgt cgagcggctg atcgcgcgcg tgccgggacg gctgtcggtg
      181 gtgtcgatcg tcggcggcac cgtgttcgcg tcgctgtcgg gctcgactat cgccaacacc
      241 gccgtgctcg gcaacctgct gctgcccgac atgctgcgcc gaggctacca tccctcgatc
      301 gcgatggggc cgatcatggc caccggcggc atcgccatgc tgatcccgcc ctcggcgctg
      361 gcggtcctgc tcggcagtct cgccggcatc tcgatctcgg ggctgctgat cgccggcatc
      421 gtgccgggcg tgatcatgag cctgctattt gtcggctatg tgatcggccg ctgcgccctc
      481 gaccgcagcc tcgcgcccgc gggcgatgat gcgccgcgca ccgagggcgg gcgctggcgg
      541 ccattcctga tctacgtgct gccgctattc ggcatcttcg ccgtggtggt cggcagcatc
      601 ttcgccggct gggcgacgcc gaccgagtcg gcggcgctcg gctgcgtcgc ctcggccgcc
      661 gccgccgcgg gctaccgcgc gctgacctgg cgcggcctga tgaccgcggt gcgcgagaca
      721 gccaagatct cgaccatgat cctgttcatc atcgtcgcct cggtcaccta tgcccagatc
      781 ctcgcctttt ccggcgccac cgacggcctg ctgcggctga tcgagggctt cggcctcggc
      841 ccgctgctgc tgctggtggt catgctgctg gtgctgctga tgctcggctg cttcatggac
      901 caggtcagca tggtgctgat caccttgccg ttcttcattc cgcttgccca ggccaaccac
      961 atcgacctgc tgtggctcgg cgttctgatg ctgatcgcga tggagatcag cttcaccacg
     1021 ccgccgttcg ggctcttggt gttcgtgatg aagggggtgg cgccgccgac tatcaccatc
     1081 cgccaggtct atgccgcggc gctgccgttc atcggcctgg aggcgctggt gttggcgatg
     1141 ctggtcgcct ggcccaagct cgcggtctgg ctgccgtcgg cgatcctcaa ccgctgaggc
     1201 gctcagccgc tgccccacac cgactcggcg accgggcttg agcacccgca caaaggctcg
     1261 gcccgctacg gcggggtcaa aggacgacgc ctcacaccca cagcttctcc ttgcggtacg
     1321 cgatgtcggt gagcaacgcc gccgggcctt cgtgggtgat ggcgccgcgg tcgagcacgt
     1381 aggcgcggtc ggcgagcgcc agcaccaggt cgaggtcgtg ctcgatgatc agcatcgaca
     1441 cgtcctggcg caaccggtcg atggcggcga acacctcctc gaccatggtc ggcgacaggc
     1501 cctcgaacgg ctcgtcgagc agcagcaggc gcacgttgcc cgacaaggcg cgggcgatcg
     1561 cgaccatctg ctgctcgccc ccggacagca cgtccgcctt ggcggcgaag cgttcctcga
     1621 tgcgcgggaa acgcgcgaac atctcctccc gcgaccagcg cgcgccgtcg ccgccggtcc
     1681 gcctgagccg gcccagggcc aggttgtcgg cgacggtgag attggcgaac agcctgcgcc
     1741 cttgcggcac caggccgatg ccgaggcgcg ccacgtcctc gggcgcgctg ccctcgatgg
     1801 cgcgaccgtc gaacacgata cgcccggtgc gcggcggcac gatgcccatg atgcccttga
     1861 cggtcgagga cttgccggcg ccgttgcgcc cgagaagcgc caccacctcg cccttgcgca
     1921 cgtcgagcga gacatcgtgc aggatgtggc tcttgccgta gaacaggttg acgttgtcga
     1981 ggcgcaggat cgactccccg ctggcgcgcg acggcgccgc gcgcctggcg gtcacgacct
     2041 gccggccgct gcccagatag acctgctgga cctcgggatg ggcgcgcacc tcgtccggcg
     2101 aaccctcggc gagcaccaga cctgcgttca tcaccgtgat cacctcggcg aacgcgaaca
     2161 cccggtcgat gtcgtgctcg accagggtaa cggacatgtg cctcgccaag gcgcggatca
     2221 ggccgacgat gcgttcgcgt tcctggtcgg cgagtccggc caacggttcg tcgagcaaca
     2281 gcacccgcgg ccgggcggcc agcgccaggc cgatctcgag caggcgctgc atgccgtaag
     2341 acaggtccga gaccgcgacg tgctcgatcc cctcgaagcc gagcaggccg atcaaggcgc
     2401 gggtctcctc gtgcacgcgc gcgaagctgc ccgcgttccg ccacaggtcg aggcggccct
     2461 tgtggcgcgc ctggacgccg agacgaaggt tctcgtggac gctgagcgtc ggaaacaggt
     2521 tggtgatctg gaacgagcgc gccaggccga ggcgcgcgag gcggtccgcc ggcaggcccg
     2581 cgaccgcgct gccgaacagc gcgagctcgc cctcgtcggg cgggtacatg cccgagatga
     2641 ggttgaacag cgtcgtcttg ccggcgccgt tggggccgat cagcgcgcgc agctcgcggt
     2701 cgcgcagctc gaggtcgacc ccggccaccg cccggaaccg gccgaagcgc ttggtcacgt
     2761 cacgacaggc gaacaccacc gcccgctcgc tgcgcgccgg gccggcaacg aggaagcggg
     2821 gaatctcggc gcgcatctcc ggcaccgcgc gcgcggccat cgccgccgcc ctgacctcgt
     2881 ggcgggtgag cggggcgagc aggcgcgtgc ccagcccgac cagccccttg ggcgagaaca
     2941 ggatgaaggc cataaacagc aggccgaagt agaactgcca ggcgctggtg tagtcggaca
     3001 ggatctcgcg gaacaggatg aagaacccgg cgccgagcgc cggcccgagg aaggcgccca
     3061 tgccgccgat gatcgacatc gccaggatct cgcccgagaa catgacgtgg accaggtcgg
     3121 cggaaacgaa gtattttagg aacgcgaaca ggcttccggc caggccgagg atcgacgccg
     3181 agaggacgaa cgcgatcagc ttgtagcggc gcaccggata gccgacgaac gacgcgcgct
     3241 gctcgttgtc gcggatcgcc tgcagtgcgc agccgaacgg cgactggaca acgcgccaga
     3301 tcagccaggc gaccagcagc acgatggcgg cgaccaggta gtagaaggtg gtctggtcgt
     3361 cgatgtcgat gccccagagg tcggggcggg cgattccgcc gagcccgttt tcgccgccgg
     3421 tgaacgaggt ccagcggtag acgacgtaga agaacatggc ggtgaaggcg agcgtgagca
     3481 gcgagaaata gaccccgcgc cggcgcaaca ccaggaagcc gatcaccagg ccgagcaacg
     3541 ccgagaacag gaccgcggcc gcgagcggaa ccaacagcgc gccgggcaga acgtggatct
     3601 ggagcagcgc cgcggcgtaa gccgcgagcc cgaagaaggc gccgtggccg aacgagacca
     3661 gcccgctgta gccgagcaac aggttgaacc ccaggccgac cagcgcgaac agcgcgatct
     3721 cggtcgcgac cgaggtggtc aggccgagcg cgggcaccag atggggaaga cccacgagcg
     3781 cggcggcgag cactaggatc gggtggcgga ggaccttcgc gccgatcatt cgagccgccc
     3841 gacgctttcg cccgccaggc cgcgcgggcg gaccagcagc accagcacca tcaacgcgta
     3901 catcgacgcc tcggcggccg gcggatagaa cagcaccgtc atgccgcgca cgacgccgat
     3961 cagcagtccg ccgaacacca cgccccagaa gctgccaagc ccgccgatca ccaccgtgac
     4021 gaaggcggcg gtgaggatct cggtgcccat cgccggctgg acgccggcca acggcgccga
     4081 caccaccccg gcgagcgccg cgagacccac cccgagcccg aagatcgcgg ccagcaccgg
     4141 gcgcagccgg atgcccagcg cgcgcaccat ctccggatcg tggacgccgg cgcgcacgat
     4201 catcccgaac gcggtccggg tcagcagcag ccaggtgccg ccgaccgcgg ccaccgccac
     4261 gcccaggatc gccagccggt aataggagta aatgaagtcg ccgaccagga gctggccgcg
     4321 cagcgccggc gggatcgcga acggcagccc ggtcgcgccc cacaccaggc gcagcgcctg
     4381 ctcggcggtg agcgcgaggc cgaaggtgaa caggaggctc aaggtcgggt cctcgcggta
     4441 gagccgcctg agcaggacgt gctcgatcgc catgccgagc gccgccaccg cgaccggcga
     4501 ggcgatcaat gcaccgccga agccgagcat cgggctgacc gtgacggcga gatacgcgcc
     4561 cagcgcatag aacgccccgt gcgccaggtt gaccacgcca ccgaggctga agatcagcga
     4621 caggccgagc gccagcatca cgtaataggc gccgatcagg agcccgttca acgtgtgctc
     4681 gagaacgatc agcatcggct ggattgtacc cctcgggcca ccggcatgcg cgcgcaggcg
     4741 cggccagtcg ggcgccgacc cgcccgatcc cttgcccgac tatgcaactg acggcggggg
     4801 gccggtcagg ccaggctcag ccggcgaacg tgcacgggtt ctcgtcccgc gtcggctgga
     4861 tcaggtcgag cggctggtcg gccgagggca ccgcctcgac gacgtcgaag atgtcccact
     4921 tgtcccgagc cgcggccggg tccttgaccc gcaccacgta catttcctgc agcagctggt
     4981 ggtcccagtc gcggaacgac ccggcgcggc tcttgagcaa atcgaagctc gcccccttct
     5041 cgaaatgctc gatcaaggcc gccgtatcgg cgctgccgac ctcgttgacc gcctgggcga
     5101 agatcttgat cgcgatatgg tcgccccagg cctggttgtc cggcggcatg ccgtggcggt
     5161 cggtgtacgc cgtggtgaat gcctgggacg cggctgtgtc aaggccgtga taccagaggc
     5221 tctgccagta cccggacagc gactcgattc ccgcggccca gaacggcacc gtgtccatca
     5281 ccccgcccgc caccggatag ggcaggccgt actcgcgata ctgcttcagg aacgtcgtct
     5341 gatcgacccc ggccaggttg atgtagacga agtcgggctg ggcctcgcgg atcttcagga
     5401 tatagccgct gtagtccggc gtgttggtcg ccaccatgtc gttattgacc agcacgccgc
     5461 cgttctgctg aagaaagcgc gacgacacgc cgtagaggtc gtggccgaac gcgtagtccg
     5521 cggtcagcat gtaccacttc gcgcccgtga tcaggttctg gcgcacctgc cactggccga
     5581 tggtcttggt atacatggtg ttgcaacctt cgacgtggaa cgtgaagcgg ttgcaattgc
     5641 tgccacgcaa agcatcggag ttgcagccgg tgttgacata gatgatcttg tagcggttgg
     5701 cctgctcggc gatcgccagg cccgaggccg agctgatctc gccgaacagg aactggacct
     5761 ggtcgcgctc gaccagcttc tgcgtcttgg tcaccgcgac cccggggttg acgctgtcct
     5821 cggagatcag ctcgagcggg cggccgagca cgccgcccgc ggcattgacc tcgtccaccg
     5881 ccatggcggc gccgcggaag ccgtattcgc cgagctggcc gaggaagccg gtgcgcggcg
     5941 tcagatgccc gacccggatg gtgtcctccg ccttcaggac ggcgggaaag ccgagctggc
     6001 cgagaaccgc gccgccggcg ccgagcgcgg cgccgcgcag gacggcgcgc cgcgagagca
     6061 ccttgccgcc gcgccggccg attttggtgt tgctgggcat ggcatcctcc ccgattgcgt
     6121 ccctgtccgt gcccggtcgc gttggccgcg accgccactt ttgcgttcaa tttgtcacag
     6181 aaaccgccgg ccggatagcg tcgccctcgg ccgcgcgccc gaccggccgg tcaagcccgc
     6241 tgcgccgggt acaagtgcat ttttgatatg tgacacatcc ttattggcta ttgaatccta
     6301 attatgagag tgatatctgg caacctcgac atcacaacgt ctatttaaga tgcctgggac
     6361 atgatcgtcg ccacccagat gcgcgccgcc cgggcgctgc tcggcatcga ccagcgccgg
     6421 ctcgccgagc tgtcggggct gtccgtgccg acgatccagc gcatggaggc gagcgagggc
     6481 atggtccgcg gcaatgtcga ttcgctgatg aaggtggtgt ccgcgctcga ggccgccggc
     6541 gtcgagctga tcggcgacgg cgccgccagc caggacggcg gccgcggcgt gcggctgaag
     6601 gcgccgccgg ccggcgccag cgcctgaccg gacccctgcc cccatgacac gccaagccga
     6661 gacggcctga ccgggcaggc gcgatgacca tcgccgtgct cctctggtgt gtcgccgtat
     6721 tgctcggcac ggcgctggcg gcgttggcgg ccgggcgatc gccggccggc accgcggtcg
     6781 tctatggcgc gagcctcgcc gcctcgctcg cggcgctgct cgcggcgctc gcggcgctca
     6841 tcgccggaga ggccacggcg cccgcggtga cgctgccgct cgggctgccc tggatcggct
     6901 cgcatttccg gctcgacgcg ctggccgcgt tcttcctggt ggtggtcaat ctgggcggcg
     6961 cggcggcatc gctctatgcg ctcggccatg gccgccacga gccggagccg gcgcgggtgc
     7021 tgccgttcta cccggcgttc ctcgccgcca tgaacctggt cgtgctggcg gacgatgcgt
     7081 tcaccttcct gttttcctgg gagctgatgt cgctcgcttc ctgggcgctg gtcatggccc
     7141 atcaccgcga cgccgacaac gcgcgcgcgg gctacgtgta cctggtcatg gcgagcttcg
     7201 gcacgctcgc gctgctgctg tgcgtcggcc ttctcgccgg gcccgacggc ggctacgcgt
     7261 tcgcggcgat ccgcgccggc gcgcaccgcc cctgggtcgc cgccgcggtg ctggcgctgg
     7321 cgctgctcgg ggccggctcg aaggcgggcc tggttcccct tcacgtgtgg ctgccgctcg
     7381 cccacccggc ggcgccgagc cacgtctcgg cgctgatgag cggggtgatg accaaggtcg
     7441 ccgtctacgg cttcgtgcgc atcgtcttcg acctgctcgg cgagccggga tggtggcaga
     7501 gcatggtggt gctggcgctc ggcggcatta ccgcggtgct cggggtgctg cacgcgctca
     7561 tgcagcacga cctcaagcgc ctgctcgcct accacacggt cgagaacatc ggcatcatct
     7621 tcatcggcct cggcctggcg ctggcgttcc aggccaacgg catgggctgg gccgcggcgc
     7681 tggcgctgac cgcggcgctg tttcacgtct tcaaccactc gctgttcaag agcctgctgt
     7741 tcttcggcgc cggcggcgtg ctcgccgcca cgcgcgagcg cgacatggag catctgggcg
     7801 gcctgatcca tcgcatgccc tacaccagct tcgccttcct ggtcggctgc gtcgccatct
     7861 cggccctgcc gccgctcaac ggcttcgtct ccgagtggct gaccttccag gcgatcctgc
     7921 agagccccga gctgccgcag tgggggctca agatcatggt gccggccgtg ggcggcatgc
     7981 tggcgctgtc ggcggcgctg gccgccgcct gtttcgtcaa ggcgttcggc gtcacctttc
     8041 tcggccggcc gcggaccgcg gcggccgcgg cggccgcaga ggtggaccgc ttctcgctcg
     8101 ccgccatgtt cgtgctcgcc ggcctgtgcc tgctcgccgg catcctgccc ggcatcgtca
     8161 tcgacgccct ggggccggtg tcccgcagcc tgctcggcac gcgcatgatg ccgcagagcg
     8221 gcctgccgtg gctgtcgatc gtgccgatcg ccgagagccg gagctcgtat aacgggctgc
     8281 tggtgttcgc cttcatcgcg gcgtcggcgt cggtcgcggt ctacgccatc caccgctggg
     8341 cgtcgcgggc gttgcggcgg gcgccggcct gggactgcgg cttcccggac ccgagcccgg
     8401 ccacccagta caccgcaggc agcttcgccc agccgatccg ccgggtgttc gggacgctgg
     8461 tgttccgcgc gcgcgagcag gtcgagatgc cgccgcccgg cgatgcgcgg cccgcgcacc
     8521 tgacggtgac gctgcgcgac ctgatctggg acggcgtcta cgcgcccgcc gcagacgccg
     8581 tcggcttcgc cgccgagcgg ctcaatcact tccagttcct gaccatccgc cgctacgtca
     8641 gcctggtgtt cctcgccctc gtcaccctgt tgctggtgct cgcgctgtgg cggtgatcct
     8701 cgacctcctc gtccagggcg cgcagatggc actggtgctg ctgctggcgc cgctgctgac
     8761 cgggctggtg cgcaaggtca aggcgcggct cttgcgtcgc cgcggcccgc cgctgatcca
     8821 gccctatcgc gacctgctca agctgttgcg caaggaggtg gtgcttgccg acagcgcgtc
     8881 gtggctgttc cgcatcacgc cctacctgat cttcgccacc acctgggtgg cggcggcgct
     8941 ggtgccgacc ttcgccaccg gcctgatgtt cagctggacc gccgacctga tcgcgatcat
     9001 tgcgctgctc gccagcgcgc gcttcttcct ggcgctcgcg ggcatggacg tcggcaccag
     9061 cttcggcggc atcggctcca gccgcgaggt catgatcgcc tcgctcgcgg agccggcgat
     9121 gctgctgatc gtgttcaccc tcgccctggt cgcgggctcg acccagctgt cgacggtggc
     9181 cgcggtgatg gcctcgcccg aggtcggcct gcgggtctcg ctcggcctgg cgctgatcgc
     9241 gctgatcatg gtggcgatcg ccgagaacgg gcgcatccca gtcgacaatc cggcgactca
     9301 tctggagctg accatggtcc acgaggcgat ggtgctcgaa tattccgggc gccatctcgc
     9361 catgatcgag ttcgcggcct cgctcaagct gctgctctac gtgtcgctga tttcgtgcct
     9421 gttcgcgccc tgggggctgg cgatcgccga tggcggcacc ctcggctacc tcatcggcgg
     9481 ggccgcctac ctcgccaagc tcgccgccgg cggcatcgtg ctcgccctgt tcgagaccgc
     9541 gatcgccaag atgcgggtgt tccgagtgcc ggagttcctc ggcgccgcgc tcatgctcgg
     9601 cctgctcgcc accttgctgc tgttcgtatc gaggagcctg taggtggatc gcctggtctt
     9661 cgacatcgcc catctgctgg ccggcggcct ggtgctggtg agcttcctgc tgctctatca
     9721 ggaccgtctg tacgcgctgc tcaacatcta cgcccttcat gccctggtgc tggcgatgtc
     9781 ggtagcctgg caggcattcg cccaaaacgc ggaccacctc tatgtcacgg ctgcgatcgc
     9841 gctcgccttc aaggcgatcc tggtgccagg ggccttgcac cggatcatcg cccgtctcgg
     9901 catccatcgc gagatcgaga ccgtggtcgg catcggcccg accatgcttg ccggcatcgg
     9961 cctggtggcc ctgtcgatgg tggtcatgct gcgggtcacg atggcggccg acccgctggc
    10021 gcgcgaggac ctcgccttcg cgctgtcggt ggtgctgctg ggcctcttga tgatggtgac
    10081 ccggcgcaac gcggtcggcc aggtggttgg attcatgtcg ctggagaacg gcctggtgct
    10141 ggcggcgacc ggcgccaagg gcatgccgct ggtggtcgag atcagcgtcg cgttctcggt
    10201 cctgatcgcg ctgatcgtga tcggcatctt cctgttccgc atccgcgaac gcttcgacac
    10261 ggtcgatgtg caggtgatcg accggcaccg gggcgagcgg ccgtgaccgc gctcggcgtc
    10321 gacgcgctga ccgcggtgct cgtgatcccg cttgcgtcgg cggcgctgct ggcgctgctg
    10381 ccgggctatc
//
LOCUS       DASWHU010000154         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_67195, whole genome shotgun sequence.
ACCESSION   DASWHU010000154 DASWHU010000000
VERSION     DASWHU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67195"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VGB88_05925"
     CDS             complement(<1..238)
                     /locus_tag="VGB88_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647015.1"
                     /translation="MSEVHPQLRALLERIAEAGYPDQTKVPVEEARRITVERARRFYG
                     PPEPVDRVEELTLPGPAGPLRARLYAAASDGPMPV"
     gene            330..2525
                     /gene="scpA"
                     /locus_tag="VGB88_05930"
     CDS             330..2525
                     /gene="scpA"
                     /locus_tag="VGB88_05930"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX9647016.1"
                     /translation="MAEFPKRSLDDWTRLAADELKGEPADRLVWHTPEGIAVKPLYTA
                     ADLAALDYVDSLPGFPPYLRGPRATMYAARPWTVRQYAGFSTAEESNAFFRRNLAAGQ
                     MGLSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLERMSVSMTMN
                     GAVLPVMAGYIVAAEEQGVPPDRLSGTIQNDILKEFMVRNTYIYPPAPSMRIVADIIA
                     YAAQHMPRFNSISISGYHMQEAGATCVQELAFTLADGIEYVRAALSRGLAVDDFAPRL
                     SFFFCLGMNFFMEVAKLRAARVLWAELMAELFKPKDARSLMLRTHCQTSGVSLTAQDA
                     YNNVVRTTVEALAGVLGGTQSLHTNALDEAVALPTDFSARIARNTQLILQEETGITRV
                     VDPLAGSYYVESLTASLVREARALIDEVEALGGMTRAVADGMPKLSIEDSATRRQARI
                     DRGEEVIVGVNTYLPERDEPVEILDIDNAAVRAQQIARLDRVRHERDQAACQQALDAL
                     TRGAGDEQANLLALTVEAVRARATVGEVSDALERVFTRHRATSHSVSGVYGAAYADDG
                     GLEAVRAEVEAFARTEGRRPRMLVAKLGQDGHDRGAKVIATAFADLGFDVDVGPLFQT
                     PEEAARQAVENDVHVVGVSSQAAAHKTLVPEMIEALRAHGAGEVIVVCGGVIPPQDHA
                     MLRAAGVAAIFGPGTNIPRAASEIVGLIEARREAPPARTKPKLRLGEGR"
     gene            2522..3520
                     /gene="meaB"
                     /locus_tag="VGB88_05935"
     CDS             2522..3520
                     /gene="meaB"
                     /locus_tag="VGB88_05935"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9647017.1"
                     /translation="MSADDLHSRAEAVRAGDRRALARAITLVESTRRQDRDAALALLD
                     ALMPATGGALRLGISGAPGVGKSTFIEALGLHLIADGHRVAVLAVDPSSALGGGAILG
                     DKTRMADLAREARAFIRPSPTGGGLGGVARRTRDAILICEAAGFDVVIVETVGVGQSE
                     IAVRDMVDMFILLIAPAAGDELQGIKRGIVEIADLLVVTKADGELGREAARTRADYQS
                     ALHLLRPASAHWTPKVLSCSALEDTGIAEMWQAACAFRQAMDGAGEIAARRRAQALHA
                     MWAEVRDGLEAELKASPEVARRLPAIEGEVASGTLSPAAAATRLLDAVVGAASKSD"
BASE COUNT          505 a         1319 c         1275 g          483 t
ORIGIN      
        1 ccaccggcat cggcccgtcg ctcgccgccg catagagccg cgcgcgcaac gggcccgccg
       61 ggcccggcag ggtcagctcc tcgacccggt cgaccggctc cggcgggccg tagaagcgcc
      121 gcgcgcgctc gaccgtgatc cggcgcgcct cctcgaccgg caccttggtc tggtcgggat
      181 agccggcctc ggcgatgcgc tcgagcagcg cgcgcagctg cgggtggacc tcggacatgg
      241 cgccccgttc tccccgtggc ctgttcgcga ttgcgaaaag gctatacagt cggggcgatg
      301 cacgccagtc ccgcgcgcga ggcgacatga tggccgaatt tccgaagcgc agcctcgatg
      361 actggacgag gctcgccgcg gacgagctca agggcgagcc tgcggaccgg ctcgtctggc
      421 acacgcccga aggcatcgcg gtcaagccgc tctacaccgc ggccgacctc gcggcgctgg
      481 actacgtcga ctcgctgccc ggcttcccgc cctatctgcg cgggccgcgc gcgaccatgt
      541 acgcggcgcg cccgtggacc gtgcgccagt atgccgggtt ctcgaccgcg gaagagtcga
      601 acgcgttctt ccgccgcaac ctcgccgccg gccagatggg tctgtcggtc gccttcgacc
      661 tcgccaccca tcgcggctac gactcggacc atccgcgcgt caccggcgac gtcggcaagg
      721 ccggggtcgc gatcgactcg gtcgaggaca tgaagatcct gttcgacggc atcccgctcg
      781 agcggatgag cgtgtcgatg accatgaacg gcgcggtgct gccggtgatg gccggctaca
      841 tcgtcgccgc cgaggagcag ggagtaccgc ccgacaggct cagcggcacc atccagaacg
      901 acatcctcaa ggagttcatg gtccgcaaca cctacatcta cccgccggcg ccgtcgatgc
      961 gcatcgtcgc cgacatcatc gcctatgccg cgcagcacat gccgcggttc aactcgatct
     1021 cgatctcggg ctaccacatg caggaagcgg gcgcgacatg cgtccaggag ctcgccttca
     1081 ccctcgccga cggcatcgag tatgtgcgcg ccgcgctgtc gcgggggctc gcggtcgacg
     1141 acttcgcgcc caggctgtcg ttcttcttct gcctgggcat gaacttcttc atggaggttg
     1201 ccaagctgcg cgcggcgcgc gtgctgtggg cggagctgat ggcggagctg ttcaagccca
     1261 aggacgcgcg ctcgctgatg ctgcgcaccc actgccagac ctctggcgtc tcgctcaccg
     1321 cccaggatgc gtacaacaac gtcgtgcgca ccaccgtcga ggcgctcgcc ggcgtgctcg
     1381 gcggcaccca gtcgctgcac accaacgcgc tcgacgaggc ggtggcgctg ccgaccgact
     1441 tctcggccag gatcgcgcgc aacacccagc tcatcctgca ggaggaaacc ggcatcaccc
     1501 gcgtcgtcga cccgctcgcc ggctcctact acgtcgagtc cttgaccgcg agcctggtca
     1561 gggaggcgcg cgcgctgatc gacgaggtcg aggcgctcgg cggcatgacg cgcgcggtcg
     1621 ccgacggcat gcccaagctc agcatcgagg actcggcgac gcggcgccag gcgcgcatcg
     1681 accgcggcga ggaggtgatc gtcggggtca acacgtacct gcccgagcgc gatgagccgg
     1741 tcgagattct cgacatcgac aacgccgcgg tgcgcgcgca gcagatcgcg cggctcgacc
     1801 gcgtgcgcca cgagcgcgat caggcggcgt gccagcaggc gctcgacgcg ctgacccggg
     1861 gtgccgggga cgaacaggcc aacctgctcg cactcaccgt cgaggcggtg cgcgcgcgcg
     1921 ccaccgtggg cgaggtctcg gacgcgctcg agcgggtgtt cacccgccac cgtgcgacca
     1981 gccacagcgt ctcgggcgtc tacggcgcgg cctatgccga cgatggcggc ctcgaggcgg
     2041 tgcgcgccga ggtcgaagcg ttcgcgcgca cggaaggccg ccggccgcgc atgctggtcg
     2101 ccaagctcgg ccaggacggc cacgaccgcg gcgccaaggt gatcgccacc gccttcgccg
     2161 acctcggctt cgacgtcgac gtcgggccgc tgttccagac cccggaagaa gccgcccgcc
     2221 aggcggtcga gaacgacgtc cacgtggtcg gcgtgtccag ccaggccgcc gcccacaaga
     2281 ccctggtgcc ggagatgatc gaggccttgc gcgcgcacgg cgcgggcgag gtgatcgtgg
     2341 tctgcggcgg cgtcatcccg ccccaggacc acgccatgct gcgtgccgcc ggcgtcgccg
     2401 cgatcttcgg tcccggcacc aacatcccgc gcgccgcgag cgagatcgtg ggcttgatcg
     2461 aggcgcgccg cgaggcgcct cccgcgcgca cgaagccgaa gctccgcctc ggcgaaggca
     2521 ggtgagcgcg gacgatctcc actcccgcgc cgaggcggtg cgtgccggcg accggcgggc
     2581 gctggcgcgc gcgatcaccc tggtcgaatc gacccggcgt caggaccggg acgccgcgct
     2641 cgcgctgctg gacgcgctga tgccggcgac cggcggcgcg ctccgcctcg gcatctcggg
     2701 cgcgccggga gtcggcaagt cgaccttcat cgaggcgctc ggcctgcacc tgatcgcgga
     2761 cggccatcgc gtggcggtgc tcgcggtcga cccgtcgagc gcgctcggcg gcggcgccat
     2821 cctcggcgac aagacgcgca tggccgacct cgcccgcgag gcccgtgcct tcatccgccc
     2881 gagcccgacc ggcggcggcc tcggcggcgt cgcgcggcgg acgcgcgatg cgatcctgat
     2941 ctgcgaggcc gcgggctttg acgtggtgat cgtcgagacc gtcggcgtcg gccagtccga
     3001 gatcgcggtg cgcgacatgg tcgacatgtt catcctgctg atcgcgccgg ccgcgggcga
     3061 cgagctgcag ggcatcaagc gcggcatcgt cgagatcgcg gacctgctgg tggtcaccaa
     3121 ggccgacggc gagcttggcc gcgaggcggc gcgcacccgc gccgactacc agagcgcgct
     3181 ccacctgctg cgcccggcgt ccgcgcactg gacgcccaag gtgctgtcat gctcggcgct
     3241 cgaggacacc ggcatcgccg agatgtggca ggccgcgtgc gccttccgcc aggcgatgga
     3301 cggcgccggc gagatcgccg cgcgccggcg cgcgcaggcg ctccacgcga tgtgggcgga
     3361 ggtgagggac ggccttgagg ccgagctcaa ggcctcgccc gaggtcgccc gccgcctgcc
     3421 cgcgatcgag ggcgaggtcg cgagcggcac cttgagcccc gccgccgccg cgacccgcct
     3481 gctcgacgca gtggtcggcg cggcgtcaaa gtcggattga tcggcgcgcg ccatggccga
     3541 gcgcgcgcgg gtggtggtga tcggcgccgg catcgtcggc tc
//
LOCUS       DASWHU010000155         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3036978, whole genome shotgun sequence.
ACCESSION   DASWHU010000155 DASWHU010000000
VERSION     DASWHU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036978"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGB88_05940"
     CDS             complement(<1..222)
                     /locus_tag="VGB88_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647018.1"
                     /translation="MTVDTLARPRGRAARAAPARAPRRLHLGCGRRHIPGWHHVDIVA
                     FPHVDTVGPVEALRDIRDDSVEVIYACHVL"
     gene            complement(219..2570)
                     /locus_tag="VGB88_05945"
     CDS             complement(219..2570)
                     /locus_tag="VGB88_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9647019.1"
                     /translation="MTATRILHYGLEQHRAGRLDAAERAYGAALAEDACNADALHLLG
                     QVRLARGDLAGALDLVRRAIAEAAAVALYHNTLGEVLRQRGEAAAADAAYAAATARDP
                     GYADAWSNRGALAKSEGRLADAARYLRHAVALDPALASAHNNLGAVLLDQGDAEGAET
                     CFRAALAEAPELAEAHGNLGNALARQRRFKAAVEAYDHALALDPGRVEIEVGRGKALA
                     DAGDFAGAVAGYQRALARAPDTVEIEHALGVALFRAGRFAEAIERFERVLARAPDHVE
                     ALVNLGNALLRRKRPEDGIVALERALALDAACDRAYFSLVAALPGECRWRRLEELAGE
                     LEARLEAVRAQRPQHGGGLLPLAFTLPYFSTDIALHRRVLETVATRVEDSLRGRAQGF
                     RHPPREDPDRRLRIGYLSPDFGDHPIGHVTVPVYGLHDRAAFEVACYATVDRSQPANP
                     YYDRIRAAADHFVDIAALTPEAGAARIHADRIDILVDLCGYMLGGRPQVFALRPAPVQ
                     VYWLGHGGGLGARYIDYVIGDPFVTPSGDDGCYMESIARLPDSFSSADRAPIAAAPMR
                     RADHGLPEDGVVFCAFNNALKIDRPVFDCWMGILAAVPGSVLWLNAARAGALRARLCV
                     AAEARGVAASRLVFADRLADKAHHLARHRLADLFVDTFTFNASTTALDALWAGLPVLC
                     VAGPHFHSRIAAGYLSAVGMPELICADRAAYRARAIALANDPAAVSALTAKLDRNRLT
                     APLFDAKRFVRHLERAYRAMWRRHAAGAPPASFDVAALAREPA"
     gene            2776..>3403
                     /locus_tag="VGB88_05950"
     CDS             2776..>3403
                     /locus_tag="VGB88_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9647020.1"
                     /translation="MAGASVATRTRAGEARCLVGAALALHRGGQLAAAEPLYRQAIET
                     DPDAAEPLHLLGTLALQTGRPGEAARLLERAAELDPDNAVIRNRLGVACAQGGRRDEA
                     RAHYRAALARAPRLAEAHNNLGVLLADDGALDEAMAHYRRAIEADPGSAEACNNLGVA
                     LARLGDGEAAERAYRQALAANPVYPDAHNNLGTLWRERGDDAAAAEQFR"
BASE COUNT          469 a         1214 c         1315 g          405 t
ORIGIN      
        1 gagcacgtgg caggcgtaga tcacctcgac gctgtcgtcg cggatgtcgc gcagcgcctc
       61 gaccgggccg accgtgtcga catgcgggaa ggcgacgatg tcgacatggt gccagccggg
      121 gatgtggcgt cgcccgcagc ctagatgcag gcggcgcggc gcccgggcgg gcgcggctcg
      181 cgccgcgcgg ccgcgcgggc gggcgagggt gtcgaccgtc atgccggctc tcgcgccagc
      241 gcggcgacgt cgaagctcgc cggcggcgcg ccggccgcat gtcgccgcca catagcgcga
      301 tacgcgcgct ccagatgccg gacgaaccgt ttggcgtcaa acagtggcgc ggtgaggcgg
      361 ttgcggtcga gcttcgccgt tagcgccgag accgcggccg gatcgttggc caaggcgatc
      421 gcgcgcgcgc ggtacgccgc ccggtcggcg cagatcagct cgggcatgcc gaccgccgac
      481 agatatccgg cggcgatgcg cgagtggaaa tgcggcccgg cgacgcacag caccggcagc
      541 cccgcccata acgcatcgag cgcggtggtc gaggcgttga aggtgaaggt gtcgacgaac
      601 aggtcggcga gccggtggcg ggcgaggtgg tgcgccttgt cggcgaggcg gtcggcgaac
      661 accaggcgcg aggcggcgac gccgcgcgcc tcggccgcaa cgcacagccg cgcgcgcaaa
      721 gcgccggcgc gggcggcgtt cagccacagc acgcttcccg gcaccgcggc caggatcccc
      781 atccagcagt cgaacaccgg ccggtcgatc ttgagcgcgt tgttgaaggc gcagaacacg
      841 accccgtcct cgggcaggcc gtggtcggcg cggcgcatcg gcgcagccgc gatcggcgcc
      901 cggtcggcgc tcgagaagct gtccggcagg cgcgcgatgc tctccatgta acagccatcg
      961 tccccggacg gggtcacgaa gggatcaccg atcacgtaat cgatatagcg cgcgccgagt
     1021 ccgccgccat gacccagcca gtagacctgg accggcgccg ggcgcagcgc gaacacctgc
     1081 ggccggccgc cgagcatgta gccgcacagg tcgaccagga tgtcgatgcg gtcggcgtga
     1141 atgcgcgcgg cgccggcctc gggcgtcagg gcggcgatgt caacgaagtg gtcggcggcg
     1201 gcgcggatgc ggtcgtaata cgggttcgcc ggctgcgagc ggtcgacggt ggcgtagcag
     1261 gccacctcga acgcggcgcg gtcgtgcagc ccgtagaccg gcacggtgac gtggccgatc
     1321 gggtggtcgc cgaagtcggg cgacagatag ccgatgcgca gccgccggtc ggggtcctcg
     1381 cggggcgggt ggcggaagcc ctgcgcgcgc ccgcgcaggc tgtcctcgac gcgcgtcgcc
     1441 accgtctcca ggacccgccg gtggagcgcg atgtcggtcg agaaataggg cagggtgaag
     1501 gccagcggca gcagcccgcc gccgtgctgc gggcgctgcg cgcgcaccgc ctcgaggcgc
     1561 gcctcgagct cgccggcaag ctcttccagc cgccgccagc ggcattcccc gggcagcgcc
     1621 gcgaccagcg agaagtaggc gcggtcgcag gcggcatcga gggcgagcgc gcgctcgagc
     1681 gcgacgatcc cgtcctcggg acgcttcctg cgcagcagag cgttgccgag attgaccagc
     1741 gcctcgacgt gatcgggcgc gcgggcgagc acgcgctcga aacgctcgat cgcctcggcg
     1801 aagcggccgg cgcggaacag cgcgacaccg agcgcgtgct cgatctcgac cgtgtcgggc
     1861 gcgcgtgcga gtgcccgttg gtagccggcg acggcgccgg cgaagtcgcc ggcgtcggcc
     1921 agcgccttgc cccggccgac ctcgatctcg acccggccgg ggtcgagcgc gagggcgtgg
     1981 tcataggcct cgactgcggc cttgaagcgg cgctggcggg cgagcgcgtt gcccaggttg
     2041 ccatgggcct cggcaagctc gggcgcctcg gcgagcgccg cccggaagca cgtctccgcg
     2101 ccctccgcgt cgccctggtc gagcagcacc gcgccgaggt tgttgtgggc gctggcgagc
     2161 gcgggatcga gcgcgaccgc gtgccggagg tagcgcgcgg cgtcggcgag ccggccctcg
     2221 ctcttggcca gcgcgccgcg attcgaccag gcgtcggcat atcccgggtc gcgcgcggtc
     2281 gcggccgcat aggcggcatc cgccgccgcc gcctcgccgc gctggcgcag cacctcgccg
     2341 agcgtgttgt gatagagcgc gacggccgcg gcctcggcga tcgcccggcg caccagatcg
     2401 agggcgccgg cgaggtcgcc gcgggcgaga cggacctgtc ccaagaggtg cagggcgtcc
     2461 gcgttgcaag cgtcctcggc gagcgccgcg ccgtaggcgc gctcggcggc gtcgagccgc
     2521 ccggcgcgat gctgctcaag gccgtagtgc agaatccggg tcgcggtcac gcgcccagca
     2581 ttggccgcat cccggtaaag actatcttaa ggtgggtgcg ccagcttgcg ctcgatcggg
     2641 gtcggtgacg gtcgagcccg agacgtcaat aagggcccga cggtgaccca tcccagtcaa
     2701 cccggccgcg ctttgcccgc gcaccgctcc ggcggtccgg ccgcggggcg gagcgccgac
     2761 gggcgcgccg cgacgatggc gggggcgagc gtcgcaaccc ggactcgcgc cggcgaggcg
     2821 cgctgcctgg tcggcgcggc gttggcgttg caccggggcg gccagctcgc cgccgccgag
     2881 ccgctttacc ggcaggcgat cgagaccgat cccgatgccg cggagccgct gcatctgctc
     2941 ggaactctgg cgctgcagac cggccggccc ggcgaagcgg cgcgcctgct cgagcgcgcg
     3001 gcagagctcg atcccgacaa cgcggtgatc cgcaaccggc tcggcgtcgc ctgcgcccag
     3061 ggcggccggc gcgacgaggc gcgcgcgcat taccgggcgg cactggcgcg cgccccgcgg
     3121 cttgccgagg cgcacaacaa tctcggcgtc ctgctcgccg acgacggcgc gctggacgag
     3181 gcgatggcgc attatcggcg cgcgatcgag gccgacccgg gctcagccga ggcgtgcaac
     3241 aatctcggcg tcgcgctcgc ccgcctgggc gacggcgagg cggcggagcg tgcctaccgt
     3301 caggcgctcg ccgccaaccc ggtttatccg gacgcgcaca acaacctcgg caccttgtgg
     3361 cgcgagcgcg gcgacgatgc cgcggccgcc gagcaattcc gca
//
LOCUS       DASWHU010000156         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1555520, whole genome shotgun sequence.
ACCESSION   DASWHU010000156 DASWHU010000000
VERSION     DASWHU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555520"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..909)
                     /locus_tag="VGB88_05955"
     CDS             complement(343..909)
                     /locus_tag="VGB88_05955"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX9647021.1"
                     /translation="MTILGEAVVLAAVAVLVIGMVWGALSDLASYEIPNTVSLVVAAA
                     FVPAALAAGLPLVQLATAFAIAAACLVGGIGLYAVGLFGGGDVKLLSAGAVWAGAHGL
                     ASYLIAVAISGGLLSLVLVIFRRLPLPARLRRAAWVRGLHDRAAGVPYGVAIAAGAIA
                     MVPELDVLAPLADGALLSQLARLAGIVA"
     gene            complement(1048..1746)
                     /locus_tag="VGB88_05960"
     CDS             complement(1048..1746)
                     /locus_tag="VGB88_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9647022.1"
                     /translation="METDDIAAPDRGLDRVKLFEGLQADQLRAIEAQCRWLTLEPTQR
                     ALGGEDRTDDIYFIVSGRVRVLNHLADGSECLLAELGVGDCFGELSAVDSQAHSAEVV
                     GHETCLVAALPTATFRGLLVEFPEVALRLIDQLSAMVRAMNARVATMANLSPRQRVYA
                     ELLRLAAPSPNGDGSWVIDRVPTHDELACAASAAKQDVAMAIGALARDGLLERRHRSL
                     VLRDQPRLRVLANM"
BASE COUNT          344 a          785 c          721 g          262 t
ORIGIN      
        1 accgggcccg gaacaatggt acggggtccg ggcttcccgg ccgatatacc ccgagacccg
       61 gtcaaaatcc attgtccgca ccacggtcca ccggcatggc ccataagctc ctcgaatcct
      121 taacgtaaag cgttgagaag cggagacaac ctcggacctg gatgtcaccg ttcggagacc
      181 gcccgggcgg ccagcgccgg ctggtgccac ccgattccgg cgccgccggg cacaacggtt
      241 tccgaatcaa cgagaatttg ccgcgctcgg gcgccggtgc gcgatcgggc tcgcgaaatt
      301 cgcgcgccgg tgcgtgtgcg cttggccgtg gctctgtatc atttatgcaa caatcccggc
      361 taatcgcgcc aattgcgaca gcagagcccc gtcggccagc ggcgcgagga cgtcgagctc
      421 ggggaccatc gcgatcgcgc cggcggcgat cgccacgcca tagggcaccc cggcggcacg
      481 gtcgtgcaat ccgcgcaccc aggccgcgcg ccgcagccgg gcgggcagcg gcagccggcg
      541 gaagatcacc agcaccagcg acagcagccc gccggaaatt gcgaccgcga tcaggtacga
      601 cgccaggccg tgagcgccgg cccagaccgc cccggccgac agcagcttga cgtcgccgcc
      661 gccgaacagg cccacggcgt acagcccgat gcccccgacc aggcacgccg cggcgatggc
      721 gaaggcggtg gcgagctgca ccagcggcag gccggcggcg agcgccgccg gcacgaacgc
      781 ggccgccacc accagcgaga ccgtattggg gatctcgtag ctggcgaggt cgctgagcgc
      841 gccccacacc atgccgatga ccagcacggc cacggcggcg aggaccacgg cttcgccaag
      901 aatcgtcatg accgggcctc gcggcgggtc actccggccc gcatccgcac cccgccggcc
      961 aggcgcgggg tcggcttcgc ttcgacggct cgatgatagg acggcgaggg agccggcggg
     1021 aagcgaccgg cgtcgatccg caccgcctca catgttggcg agcacgcgca ggcgcggctg
     1081 gtcgcgcagc accaatgacc ggtgccggcg ctcgagcagg ccgtcgcggg cgagtgcgcc
     1141 gatcgccatc gccacgtcct gcttggcggc gctggccgcg caggcgagct cgtcatgggt
     1201 cggcacccgg tcgatgaccc agctgccgtc gccgttcggg ctcggtgccg ccaggcgcag
     1261 cagctcggca tagacccgct ggcgcggcga caggttcgcc atcgtggcga cgcgcgcgtt
     1321 catcgcccgc accatcgcgg acagctggtc gatcaggcgc agcgcgactt cgggaaactc
     1381 gaccagcagg ccgcggaacg ttgccgtcgg cagggccgcg accaggcagg tctcgtggcc
     1441 gacgacctcg gccgagtgcg cctggctgtc gaccgccgac agctcgccga agcagtcgcc
     1501 gacgccaagc tcggccagca ggcattcgct gccgtcggcc aggtgattga gcacccggac
     1561 ccggccggag acgatgaagt agatatcgtc ggtgcgatcc tcgccaccga gcgcccgctg
     1621 agtcggctcc agggtcagcc aacggcattg ggcctcgatc gcccgcagtt ggtccgcttg
     1681 caggccctcg aacagcttga cgcggtccag gccgcgatcg ggcgcagcga tgtcgtcggt
     1741 ctccatcttc ggcgaacgtc ccgtcctagg gcctcgtcac gacacggcac ccccggtgcc
     1801 cgcctcgaca acatggcgcc gcccggcacg cgcggtatcc gggtggggct tcaactcacc
     1861 cgacgcccgc gggacaccgc caaactggcg cctcgccaac agaaaatagg ccggccgccg
     1921 ctccggccaa gcgcgcccgg tcgctggccg cggcctcggt cgggggccct tattccacga
     1981 ccgcgcgcca gcctgtaacg tcccggcccg ggacaggacc gccgcacgcc gcctgagggc
     2041 atggatgcca ctgcacggca ccaccggccg acatcggatc tacctcatgc gccacggcga
     2101 ggtgcgggac tt
//
LOCUS       DASWHU010000157         4789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3386657, whole genome shotgun sequence.
ACCESSION   DASWHU010000157 DASWHU010000000
VERSION     DASWHU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3386657"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..889)
                     /locus_tag="VGB88_05965"
     CDS             complement(14..889)
                     /locus_tag="VGB88_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647023.1"
                     /translation="MTRLRTAIFALLFTGLAAPAASADLDVERTLTVTGIDRSYCLYR
                     PDSYREGAPVVFYFHGGLGDCHLEDADAWRDKADAEGFLLVAPNGLNKDWNACSAPPE
                     RCHAPSTLDEYLNVDDLAFFDAVSDLVDAEYLPGAHYAAGMSKGGMFVYHLLCHRAGA
                     IDAAAVMAGTELSQTCAGESVPLLHLHGLDDRYVPWLGGGLMGMEFSPVPPAIEAQAE
                     RNGCAGETVSQRETRDATSYRYQGCDSETVYWRYRCQHGLPGITTGWWWWMFGLGCTG
                     SFHAEDPIWAFFARQ"
     gene            complement(1199..2320)
                     /gene="flhB"
                     /locus_tag="VGB88_05970"
     CDS             complement(1199..2320)
                     /gene="flhB"
                     /locus_tag="VGB88_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX9647024.1"
                     /translation="MAYEDQAEKTEEPTAKKLKDARDKGQVPKSREFNTWLILLAATL
                     VILALGPSIARSVAGALAGFIDHPHEMATDFESLRLALSHTLMRVGLALGLAAIIMVA
                     AAAGAGLLQHGPVWSVESLKPETDKISLIKGFRRQFSVKSLVEFAKGIVKLVVVGAVA
                     LAVLWPHLHTLPLLVGADMEVVLERMYWLALELLAGVIAAATVIAGLDLLYQRYEFSR
                     SQRMSRRDIRDEHRQTEGDPMVRARLRSIRMEKARQRMIAAVPEADVVVTNPTHLAVA
                     LKYDEVEMEAPTLVAKGADLVAERIRAVAREHEVPIVENPPLARALFQVELDRTIPIE
                     TYKAVAEVISYVWRLKGHQRRDRPEPAAGPGRAGQAPPR"
     gene            complement(2323..3087)
                     /gene="fliR"
                     /locus_tag="VGB88_05975"
     CDS             complement(2323..3087)
                     /gene="fliR"
                     /locus_tag="VGB88_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX9647025.1"
                     /translation="MLATLLTDQVFAFALVFVRLGAALMLLPTVGESAVPARIRLLFA
                     LSFTLVLTPLLAGRLPAPPDGALELAMVVVGEVVIGLFIGAGARIVMSALHVAGMVFS
                     FHSGLAAAQMFDPNQSVQTSISGNFLNLLALMVIFVTDLHHLLLAGLVDSYAAFPAGS
                     PWPAADGAEALAHLVGDTFRVGLQIAAPVLVAGFLLYLGAGLLNRLMPQMMVFFVVLP
                     VQMVLAFAILMVSLSAALAWFTSYFDDTWLALTVPG"
     gene            complement(3119..3382)
                     /gene="fliQ"
                     /locus_tag="VGB88_05980"
     CDS             complement(3119..3382)
                     /gene="fliQ"
                     /locus_tag="VGB88_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX9647026.1"
                     /translation="MTALEVLDIGQDAIFTLIKVILPIMLVALSVGVLISLFQALTQM
                     QEVTLSFVPKIIAVFVSLTLLLPYMLEVLLDFSNRQIERIVGL"
     gene            complement(3400..3723)
                     /locus_tag="VGB88_05985"
     CDS             complement(3400..3723)
                     /locus_tag="VGB88_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX9647027.1"
                     /translation="MAEISFASAAAAYASGLAEAARARPDGPAAATEARAAGFADMVA
                     DALEVARASGQAAEQDAGRALAGEIGLGEVVTSVTNAEITLQTVVALRDRVINAYNDI
                     LRMPI"
     gene            complement(3804..4214)
                     /gene="flgC"
                     /locus_tag="VGB88_05990"
     CDS             complement(3804..4214)
                     /gene="flgC"
                     /locus_tag="VGB88_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX9647028.1"
                     /translation="MDLLKSLFIAASGMQSQGERMRVVAENLANASSVALGPDGTPYR
                     RKTVTFANELDRTLGVELVKVARIGRDSSPFTLKYEPGHPAANAAGYVAYPNVNALVE
                     MMDLREAQRSYQANLRVLETTRALASRTLDLLRG"
     gene            complement(4240..4644)
                     /gene="flgB"
                     /locus_tag="VGB88_05995"
     CDS             complement(4240..4644)
                     /gene="flgB"
                     /locus_tag="VGB88_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX9647029.1"
                     /translation="MNLDDIAVIGLIARRMTWLGRNHDVLAQNIANADTPGYQARELK
                     PQAFGQTLAAAAGPVGLAVTAPGHMAGLGLRTPEGTRPQRDPYEVTLTGNSVSIEEQA
                     VKIARNAMDYQLATTLYGKAVDMLRVVTGNRR"
BASE COUNT          749 a         1663 c         1696 g          681 t
ORIGIN      
        1 ccgggcgggg cggtcactgg cgcgcgaaga acgcccagat cgggtcctcg gcgtggaacg
       61 agccggtgca gccgaggccg aacatccacc accaccagcc ggtcgttatg ccgggcaagc
      121 cgtgctggca gcggtagcgc caatagaccg tctcgctgtc gcacccctga tatcggtagc
      181 tggtcgcatc gcgcgtctcg cgctgcgata ccgtctcgcc cgcgcagccg ttgcgctccg
      241 cctgcgcctc gatggcgggc ggaacgggtg aaaattccat gcccatgagg ccgccgccga
      301 gccacggcac gtagcggtca tcgaggccgt ggagatggag caacggcacc gactcgccgg
      361 cgcaggtctg gctcagctcg gtaccggcca tgaccgcggc ggcatcgatc gccccggcgc
      421 ggtgacagag caggtgatag acgaacatcc cgcccttcga catgccggcc gcgtagtgcg
      481 cgccgggcag atactcggcg tcgaccaggt cgctgacggc gtcgaagaac gccaggtcgt
      541 cgacgttcag gtactcgtct aaggtgcttg gcgcatggca gcgctccggc ggcgcgctgc
      601 acgcattcca gtccttgttg agcccgttgg gcgcaaccag caggaagcct tcggcgtcgg
      661 ccttgtcgcg ccaggcatcg gcatcctcca ggtgacagtc gccaaggccg ccgtggaaat
      721 agaacaccac cggggcgccc tcccggtagg agtccggccg gtacaggcag tagctccggt
      781 cgatgcccgt caccgtcagc gtgcgctcga cgtcgaggtc cgccgaggcc gccggcgccg
      841 ccaagccggt gaacagaagt gcgaagattg cagtccgtaa gcgagtcatt ccccggtcct
      901 ccggtgagcc caatccggcg acgggccgcc tacgagaacg ggagagcgag ccacctggca
      961 tgccgaaccc cgggcaaccc cgagaccggc tcgcggcctc cgccacaggg gcgcgggagc
     1021 cccctcggcg caggccggcg cgatcgtttt cgtaggcggt ccaccgcccc ggtagcagat
     1081 agggccttgc taccttttgg acactctgtc ccggcatcca cacgtgatgg tggtcacagc
     1141 cgaaacaggt cctgccgggc cgcgggaggt tggggacggg acggcgcggg cgcgaggctc
     1201 agcgcggcgg cgcctggcct gcccggccgg ggcccgccgc cggttcgggc cggtcgcggc
     1261 gctggtggcc cttgagccgc cagacgtagg agatcacctc ggcgaccgcc ttgtaggtct
     1321 cgatcgggat ggtgcggtcg agctcgacct ggaacagggc gcgggcgagc ggcgggttct
     1381 cgacgatcgg cacctcgtgc tcccgggcga ccgcgcggat gcgctcggcg accaggtccg
     1441 cgcccttggc gaccaaggtc ggcgcctcca tctcgacctc gtcatatttg agcgcgaccg
     1501 cgaggtgggt cgggttggtg accaccacgt cggcctcggg caccgccgcg atcatgcgct
     1561 ggcgcgcctt ctccatgcgg atcgagcgca gccgcgcgcg caccatcggg tcgccctcgg
     1621 tctgcctgtg ctcgtccctg atgtcgcgcc gcgacatgcg ctggctacgg ctgaactcgt
     1681 agcgctgata gagcaggtcg aggccggcga tcacggtcgc cgccgcgatc acgccggcca
     1741 gcagctcgag cgcgagccag tacatgcgct cgagcaccac ctccatgtcg gcgccgacca
     1801 gcagcggcag ggtgtggaga tggggccaca gcaccgccag cgcgaccgcg ccgaccacca
     1861 ccagcttgac gatgcccttg gcgaactcga ccagcgactt gaccgagaac tggcggcgga
     1921 agcccttgat cagcgagatc ttgtcggtct cgggcttgag cgattccacg ctccacaccg
     1981 ggccgtgctg gagcaggccc gcgccggccg ccgccgccac catgatgatc gcggcaaggc
     2041 cgagcgcgag gcccacccgc atcagcgtgt ggctgagcgc aaggcgcagc gattcgaagt
     2101 cggtcgccat ctcgtgcggg tggtcgatga agccggcgag cgcgccggcg acgctgcgcg
     2161 cgatcgaggg gcccagcgcc aggatcacca gcgtcgccgc gagcaggatc agccaggtgt
     2221 tgaactcgcg cgacttcggc acctggccct tgtcgcgcgc gtccttgagc ttcttcgctg
     2281 tcggctcttc ggttttttcg gcttggtctt cgtaggccat ggctagcccg gcaccgtgag
     2341 cgcgagccag gtgtcgtcga agtagctcgt gaaccaggcg agcgccgccg acagcgacac
     2401 catcaggatg gcgaaggcga gcaccatctg caccggcagg acgacgaaga acaccatcat
     2461 ctgcggcatc agccggttga gcaggccggc gccgagatag agcaggaagc ccgcgaccag
     2521 caccggcgcc gcgatctgga ggccgacccg gaaggtgtcg ccgaccaggt gcgcgagcgc
     2581 ttccgcgccg tccgccgccg gccacgggct gccggcggga aaggcggcgt aggagtcgac
     2641 cagtccggcg agcaagagat ggtgcaggtc ggtgacgaag atcaccatca gcgccagcag
     2701 gttgaggaag ttgccgctga tcgaggtctg gaccgactgg ttggggtcga acatctgggc
     2761 ggcggcgagg cccgagtgga acgagaacac catgcccgcg acgtgcagcg ccgacatcac
     2821 gatgcgcgcg ccggcgccga tgaacagccc gatcaccacc tcgccgacca ccaccatggc
     2881 gagctccagc gcgccgtcgg gcggcgccgg caggcggccg gcgagaagcg gcgtcagcac
     2941 caaggtgaaa gacagcgcga acaacaggcg aatgcgcgcc ggcaccgccg actcgcccac
     3001 cgtcggcagc agcatcagcg ccgcgccgag ccgcacgaac accagcgcga aggcgaagac
     3061 ctggtcggtg aggagagtgg cgagcaccgc tgtccgcccc gcccgcgccg cgcgcccgct
     3121 acaggccgac gatgcgctcg atctgccggt tggagaagtc gagcaggacc tcgagcatgt
     3181 atggcagcaa cagcgtcaac gacacgaaca cggcgatgat cttgggcacg aaggagagcg
     3241 tcacctcctg catctgggtg agcgcctgga acagcgagat caagacgccc accgacagcg
     3301 ccaccagcat gatcggcagg atcaccttga tcagggtgaa gatcgcgtcc tggccgatgt
     3361 cgaggacttc gagcgcggtc accgccggcc cccgttcgct cagatcggca tgcgcaggat
     3421 gtcgttgtag gcgttgatca cccggtcgcg cagcgcgacc acggtctgca aggtgatctc
     3481 ggcgttggtc accgaggtca ccacctcgcc caggccgatc tcgccggcga gcgcgcgccc
     3541 ggcatcctgc tcggccgcct gcccgctcgc gcgcgccacc tcgagcgcgt ccgcgaccat
     3601 gtcggcgaag ccggcggcgc gcgcttcggt cgcggcggcg ggcccgtccg ggcgcgcccg
     3661 cgcggcctcg gcaagcccgc tggcataggc ggcggcggcg gaagcgaagc tgatctcggc
     3721 catgtcgaac cctcctgtcg gtcgcgtgct cgccggccag tctcagcgcg gccggtaaac
     3781 aaacccttag gccggcgcgc cgctcagccg cgcagcaggt cgagggtgcg cgaggcgagc
     3841 gcgcgcgtgg tctcgagcac gcgcaggttc gcctggtagc tgcgttgcgc ctcgcgcagg
     3901 tccatcatct cgaccagcgc gttgacgttc ggataggcga cgtagcccgc ggcattcgcc
     3961 gccgggtggc cgggctcgta tttgagcgtg aacgggctcg aatcgcgccc gatccgcgcc
     4021 accttgacca gctcgacgcc gagcgtgcgg tcgagctcgt tggcgaaggt cacggtcttg
     4081 cgccggtacg gcgtgccgtc ggggccgagc gcgaccgagc tggcgttggc caggttctcg
     4141 gcgaccacgc gcatgcgctc gccctggctc tgcatgcccg aggcggcgat gaacagcgac
     4201 ttgagcaggt ccatggtcgg gactcctttc aggtgcacgc tagcggcggt tgccggtgac
     4261 cacgcgcagc atgtcgaccg ccttgccgta aagggtggtg gcgagctggt agtccatggc
     4321 gttgcgcgcg atcttgaccg cctgctcctc gatcgacacc gagttgccgg tgagcgtcac
     4381 ctcgtaggga tcgcgctggg gacgcgtgcc ctcgggcgtg cgcagtccga ggccggccat
     4441 gtggccgggc gcggtgaccg ccagcccgac cgggccggcc gccgcggcga gcgtctgccc
     4501 gaacgcctgc ggcttcagct cgcgcgcctg gtagcccggc gtgtcggcgt tggcgatgtt
     4561 ctgggcgagc acgtcgtgat tgcgcccgag ccaggtcatg cgccgggcga tgagcccgat
     4621 cacggcgatg tcgtcgaggt tcatggtccg ccctcctgcg ccgcggcgaa ggccaaggct
     4681 cgcgcggcgc gcttaacttt cggtaaagcg cacatgcaac ccgcttgcca accgcgcgcc
     4741 ggctcgcgtc ccgccgccct cggccgcggc ggccgcgggc ttcgaaggt
//
LOCUS       DASWHU010000158         7099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2301789, whole genome shotgun sequence.
ACCESSION   DASWHU010000158 DASWHU010000000
VERSION     DASWHU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301789"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="lptE"
                     /locus_tag="VGB88_06000"
     CDS             <1..417
                     /gene="lptE"
                     /locus_tag="VGB88_06000"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX9647030.1"
                     /translation="PARALALVAISPIEDSDLGPALRDALAENLAPRGSNVRPRYQLA
                     IQLITSRAPQITEKDSLIRRYDLIVSADYALTDPVTGAALDAGTVKSVTSYNIIEAEP
                     FASLAAEKSAGEKAAREIGREIVNRLALYFRRQAPS"
     gene            428..1462
                     /gene="holA"
                     /locus_tag="VGB88_06005"
     CDS             428..1462
                     /gene="holA"
                     /locus_tag="VGB88_06005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9647031.1"
                     /translation="MKVGARDGDRFAGKPPAGIVAVLVYGPDRGLVRERAERLARTVV
                     GDLADPFRVGELQAAALAGDPAALADEARAISLVGGRRLVRVRAAGEEVAPACRNLLA
                     DPAGDSLVIVEAGELGRSSVLRKLFESHARAAALACYLDDGATLDAVIRDALAAHSLT
                     AAPGARAYLAQHLGGDRGLTRAEIDKLALYVGEGGKVSEDDARACVGDSAEITLDHLA
                     DAVAAGDAAGCHRALERAAHEGTTPVSVLRALERHFQRLQQAKAMVAAGARADEAVKA
                     LKPPVFFKRERAVAGQVARWPADALAGALDVLLQAEIDCKTTGLPDRAVCGQAALRLT
                     VAAQRQAGRG"
     gene            complement(1662..2531)
                     /locus_tag="VGB88_06010"
     CDS             complement(1662..2531)
                     /locus_tag="VGB88_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9647032.1"
                     /translation="MSARERGLGRGLGALLGESAGQTGAGGDGGQLMVPIGALSRGPF
                     QPRRRFDAEQLEALAASIRERGIMQPILVRSDHGRAGHYQIVAGERRWRAAQLAKLHE
                     VPVIVRALHDRDALEIAIIENVQREDLGPLEEAEGYRRLIEEFRATQDDIAREVGKSR
                     SHVANMLRLLALPAAVRAQLDDRRLSAGHARALLGAADPVALASKVIARGLNVRQTER
                     LVQQEQRAPKRAAVRGKSADVAKLEHDLSEALGLHVTIADRGEAGELRIAYRSLEQLD
                     ALIALLKGPLDIR"
     gene            complement(2528..3376)
                     /locus_tag="VGB88_06015"
     CDS             complement(2528..3376)
                     /locus_tag="VGB88_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9647033.1"
                     /translation="MSIAAEPAIGPGAGARVIAVANQKGGVGKTTTAINLATALAAIN
                     KRALVVDLDPQGNASTGFGIGVAARVRTTYDVMMGEASIEDAAMATAVPGLWLVPSEV
                     DLTGVEFELVAEADRVLRLRQALSSELRRYDYILIDCPPGVTLLTINALVAAKTVLVP
                     LQCEFFALEGLSQILHTIERVRALYNPGLAIEGVVLTMFDRRNNLSEQVAADVRQFLG
                     EKVFETVIPRNVRVSEAPSHGKPVLLYDFRSAGARAYMYLVSELLRREHNKMRAGAMA
                     IGQAAP"
     gene            complement(3360..4001)
                     /gene="rsmG"
                     /locus_tag="VGB88_06020"
     CDS             complement(3360..4001)
                     /gene="rsmG"
                     /locus_tag="VGB88_06020"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9647034.1"
                     /translation="MSVAAPLDAEGFARLTGVSRETLANLETYAGLLARWQRRINLVG
                     RSTLADLWRRHMLDSAQLAPLVPAQARTMCDIGAGAGFPGLVLAIVLAERLAEVSLIE
                     ADARKGAFLGEVIRATGAPARVVTARAEQCPIAPVEVVVARACAPLDRLLGYAAPLLL
                     PGGIALLLKGREVDRELTEARNHWTMTVVRHPSRTHPGGTILEVRTLARVDRR"
     gene            complement(3998..5872)
                     /gene="mnmG"
                     /locus_tag="VGB88_06025"
     CDS             complement(3998..5872)
                     /gene="mnmG"
                     /locus_tag="VGB88_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9647035.1"
                     /translation="MRSGDSYDVIVVGGGHAGCEAAAAAARVGARTALLTHRLDTIGA
                     MSCNPAIGGIAKGTLVREVDALGGLMGLAADRAGIQFRVLNASKGPAVRGPRAQADRK
                     LYAGAVQALLAGQDGLCLGAAAVEDLVLGADGAVAGVVTAAGEALRAARVVLTTGTFL
                     CGVIHIGDERVAAGRVGEAPAVGLARTLARLGFRLARLKTGTPPRLDGRTIDWSGLEV
                     QAGDVPPKPFSFLTDSIATPQVPCHLTATTPATHAIIRANLDRSPVFSGAIAGIGPRY
                     CPSIEDKVVRFAERARHQVFLEPEGLDDDTVYPNGISTSLPRAVQAALVATIPGLGRA
                     RILRPGYANEYDFVDPRELRPTLETRKVPGLYFAGQINGTTGYEEAAAQGLIAGLNAG
                     LAAAGSPAFVLDRADALIGVLIDDLVTLGTREPYRMFTSRAEYRLSLRPDNADLRLTP
                     RGIARGCVGGARARRFEAKRRAVAAARARLAALTLTPDQAARHGLSLRFDGVVRSGLD
                     LLAHAGIGLARLAAVWPELAGLPAEVAEQVEVEARYAPYLARQAAEIAAFRKDSALAV
                     PDDLDFRAVGGLSAEVTAKLEEARPRSLAAAARLPGVTPAALTALLVHIRGRALGAQA
                     "
     gene            complement(5956..>7099)
                     /gene="mnmE"
                     /locus_tag="VGB88_06030"
     CDS             complement(5956..>7099)
                     /gene="mnmE"
                     /locus_tag="VGB88_06030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9647036.1"
                     /translation="ALVLWFPGPASFTGEDVAELHLHGGRAVVAGVLEALAALPGLRP
                     AEAGEFSRRAFVNGKLDLTAAEGLADLVAAETEGQRRQALRQLDGALGRLYDGWRERL
                     VRTLAHLEAGLDFADEPLPGGLAAAALAEAQAVAAEIAGHLDDGRRGERLRDGAEVAI
                     VGPPNAGKSSILNQLAQRDVAIVSARAGTTRDVIEVHLDLAGVPVTVADTAGLREAGG
                     EVEREGVRRARARAETADLVVLVLDASGPIDPDAQARALLARNSLVVLNKTDLVAGAL
                     PGSVCGRPALATSCKTGAGIAGLVAAIAGRCEAGSGDGPPITRARHRAALQACAEALA
                     RAQGADAAELAAEDLRHAVTALGRITGRVDVEDILDVIFAEFCLGK"
BASE COUNT          962 a         2605 c         2615 g          917 t
ORIGIN      
        1 ccggcgcgcg cgctcgcgct ggtcgcgatt tcaccgatcg aggattccga cctcggcccg
       61 gcgctgcgcg acgccctggc cgagaacctg gcgccgcgag ggtccaacgt ccggccgcgc
      121 tatcagttag cgatacaatt aattacctcg cgcgccccgc agataaccga gaaggattcg
      181 ttgattcgcc gctacgacct gatcgtctcg gcagactacg cgctgaccga cccggtcacc
      241 ggcgccgcgc tcgatgccgg cacggtgaag tcggtgacca gctacaacat catcgaggcc
      301 gagccgttcg cgagcctcgc cgccgagaag agcgcgggcg agaaggcggc gcgcgagatc
      361 ggccgcgaga tcgtcaatcg cctggcgctc tacttccgcc gccaggcgcc gtcatgagcg
      421 ggccgcgatg aaggtcgggg cgcgcgacgg cgaccgcttc gcgggcaagc cgcccgcggg
      481 catcgtcgcg gtgctcgtct acggccccga ccgcggcctg gtgcgcgagc gcgccgagcg
      541 gctcgcgcgc acggtggtcg gcgacctcgc cgatccgttc cgcgtcggcg agctgcaggc
      601 cgctgcgctc gccggcgatc ccgcggcgct cgccgacgag gcgcgcgcga tctcgctggt
      661 cggcgggcgg agactggtgc gcgtgcgcgc cgccggcgag gaggtcgcgc ccgcgtgccg
      721 gaacctgctc gccgatccgg ccggcgattc gctggtgatc gtcgaggcgg gcgagctggg
      781 taggtcctcg gtgctgcgca agctgttcga atcgcacgcc cgcgccgccg cgctcgcgtg
      841 ctatctcgac gacggggcca ccctcgatgc ggtgatccgc gacgcgctcg cggcccattc
      901 gctcaccgcc gccccgggcg cgcgcgccta tctcgcccag catctcggcg gcgaccgcgg
      961 gctgacccgg gccgagatcg acaagctcgc gctctatgtc ggcgagggtg gcaaggtgag
     1021 cgaggacgac gcgcgcgcct gcgtcggcga cagcgccgag atcaccctcg accacttggc
     1081 cgacgcggtc gccgcgggcg acgccgccgg ctgccaccgg gcgctcgagc gcgcggccca
     1141 cgaaggcacc acgccggttt ccgtgctgcg cgccctcgag cgccacttcc agcgcctgca
     1201 gcaggcgaag gcgatggtcg cggcaggcgc gcgcgccgac gaggcggtga aggcgctcaa
     1261 gccgccggtg ttcttcaagc gcgagcgcgc ggtcgccggc caggtcgcgc gctggccggc
     1321 ggatgcgctc gccggcgcgc tcgacgtcct gctccaggcc gagatcgact gcaagaccac
     1381 ggggctgccc gaccgggcgg tgtgcggcca ggccgcgctc cgcctgaccg tcgcggcgca
     1441 gcgccaggcc gggcgcggct agagcgtgat gatttgaaac ctggccatcc ttgacccgtc
     1501 acccaggcca aggcgcgaag cgccgtgagc cggggtccat ctcgccgctt gcgcgagctg
     1561 ccggttggtt gcaaaggcgt atggaacccg gataacggct tcgccgcttc cggggtgacg
     1621 accaagaaaa tcccggcgat tcgacgaaat atcatcccgc tctagcggat gtcgagcggg
     1681 cctttgagca gggcgatcag cgcatcgagc tgctcgagcg agcgataggc gatgcgcagc
     1741 tcgccggcct cgccgcggtc ggcgatggtc acgtgcaggc ccagcgcctc gctcaggtcg
     1801 tgctcgagct tggcgacgtc ggcgctcttg ccgcgaacgg cggcccgctt gggcgcgcgc
     1861 tgctcctgct gcaccaggcg ctcggtctgg cgcacgttga gcccgcgcgc gatcaccttg
     1921 cttgccagcg ccaccggatc ggcggcgccg agcagcgccc gggcgtggcc ggcgctgagc
     1981 cggcggtcgt cgagctgggc gcgcacggcc gccggcaagg cgagcaggcg cagcatgttg
     2041 gcgacgtggc tgcggctctt gccgacctcg cgcgcgatgt cgtcctgggt ggcgcggaac
     2101 tcctcgatca ggcgccggta gccttcggcc tcctcgagcg ggcccaggtc ctcgcgctgc
     2161 acgttctcga tgatcgcgat ctcgagcgcg tcgcggtcgt ggagggcgcg cacgatcacc
     2221 ggcacctcgt gcagcttggc gagctgcgcc gcccgccagc gccgctcgcc ggcgacgatc
     2281 tggtagtggc cggcgcggcc gtggtcgctg cgcaccagga tcggctgcat gataccgcgc
     2341 tcgcggatcg acgccgccag cgcctcgagc tgctcggcat cgaaccggcg ccgcggctgg
     2401 aacggcccgc ggctgagcgc gccgatcggc accatcagct ggccgccgtc acccccggca
     2461 cctgtctggc ccgcgctctc gccgagcagc gcgccgagcc cccggccgag gccgcgctcg
     2521 cgcgcgctca tggcgccgcc tggccgatcg ccatcgcgcc cgcgcgcatc ttgttgtgct
     2581 cgcggcgcag cagctcgctg accaggtaca tgtaggcgcg cgcgcccgcc gagcggaagt
     2641 cgtagagcag caccggcttg ccgtgggatg gcgcctctga cacgcgcacg ttgcggggga
     2701 tcactgtctc gaacaccttt tcgccaagaa actggcggac atctgcggcc acttgctcgg
     2761 ataagttgtt gcggcgatcg aacatcgtca gcaccacgcc ctcgatcgcg agccccgggt
     2821 tgtacagcgc ccgcacccgc tcgatggtgt gcaggatctg gctcaggccc tcgagggcga
     2881 agaactcgca ttgcagcggc accagcacgg tcttggccgc gaccagggcg ttgatggtca
     2941 gcagcgttac ccccggcggg cagtcgatta ggatgtagtc gtagcggcgc agctcggacg
     3001 acagcgcctg gcgcaggcgc agcacccggt cggcctcggc gaccagctcg aactcgaccc
     3061 cggtcaggtc gacctcggac ggcaccagcc acagtccggg caccgcggtg gccatggccg
     3121 cgtcctcgat cgaggcctcg cccatcatca cgtcgtaggt ggtgcggacg cgcgcggcga
     3181 cgccgatgcc gaagccggtg ctggcgttgc cctggggatc gaggtcgacc accagcgccc
     3241 gcttgttgat cgccgccagc gcggtggcca ggttgatcgc cgtggtcgtc ttgccgacgc
     3301 cccccttctg gttggcgacc gcaatgacgc gcgcgcccgc ccccggcccg attgcgggct
     3361 cagcggcgat cgacacgagc gagagtcctc acttcgagta tggttccccc ggggtgcgtc
     3421 cggctgggat gccgcaccac ggtcattgtc cagtgattcc tcgcctcggt caattcccga
     3481 tcgacctcgc gccctttcag caacaaggcg atgccgcccg gcaacagcag cggcgcggca
     3541 tagccgagca accggtcgag cggcgcgcag gcgcgcgcca ccaccacctc gaccggcgca
     3601 atcgggcact gctcggcgcg cgcggtgacc acccgcgccg gcgcacccgt agcgcgaatc
     3661 acttcgccga ggaaagcgcc cttgcgcgcg tcggcctcga tcagcgaaac ctcggccagc
     3721 cgctcggcga gcacgatcgc cagcaccagg cccgggaagc cggcgccggc gccgatatcg
     3781 cacatggtgc gcgcctgcgc ggggaccagc ggcgcaagct gggcgctgtc gagcatgtgc
     3841 cggcgccaca ggtcggcgag ggtgctgcgg ccgaccaggt tgatccgccg ctgccagcgc
     3901 gcgagcaatc cggcataggt ctcgagattg gccaatgttt cacgtgaaac accggtgagc
     3961 cgtgcgaacc cctcggcgtc gagcggcgcc gcgacgctca cgcctgggcg ccgagcgcgc
     4021 ggccgcggat gtgcacgagc agcgcggtca gcgccgccgg ggtcacgccg ggcaggcgcg
     4081 cggcggcggc gagcgagcgc gggcgcgcct cctcgagctt ggcggtgacc tcggccgaca
     4141 ggccgccgac ggcgcggaaa tcgaggtcgt cgggcaccgc cagcgccgag tccttgcgga
     4201 atgccgcgat ctcggccgcc tggcgggcga gatagggcgc atagcgggcc tccacctcga
     4261 cctgctcggc gacctcggcc ggcagaccgg cgagctcggg ccacaccgcc gccagccggg
     4321 cgaggccgat gccggcatgg gcgagcaggt cgaggcccga gcgcaccacg ccgtcgaagc
     4381 ggagcgaaag cccatgccgc gccgcctggt ccggggtgag cgtcagcgcc gcaagccgcg
     4441 cgcgggcggc ggcgacggcg cgccgcttgg cctcgaaccg ccgggcgcgc gcgccgccga
     4501 cgcagccgcg cgcaatcccg cgcggcgtca gccgcaggtc ggcgttgtcg ggcctgagcg
     4561 acagccggta ctcggcgcgc gaggtgaaca tgcggtaggg ctcgcgcgtg cccaaggtga
     4621 ccagatcgtc gatcagcacc ccgatcaggg cgtcggcgcg atcgagcacg aaagcgggcg
     4681 atccggccgc ggcgaggccg gcattgaggc cggcgatgag gccctgggcg gcggcctcct
     4741 cgtagccggt ggtgccgttg atctggccgg cgaaatagag gcccggcacc ttgcgcgtct
     4801 ccaaggtcgg gcgcagctcg cgcggatcga cgaagtcgta ctcgttggcg tatcccgggc
     4861 gcaggatgcg cgcgcggccg aggcccggga tggtcgcgac cagcgccgcc tggaccgcgc
     4921 gcggcagcga ggtcgagatg ccgttgggat agacggtgtc gtcgtcgagc ccctcgggct
     4981 cgaggaacac ctggtggcgg gcgcgctcgg cgaagcggac caccttgtcc tcgatcgacg
     5041 ggcaatagcg cgggccgatt cccgcgatcg cccccgaaaa gaccggcgag cggtcgagat
     5101 tggcgcggat gatggcgtgg gtcgctggcg tggtcgcggt cagatggcac ggcacctgcg
     5161 gcgtggcgat cgaatcggtc aggaacgaga acggcttcgg cggcacgtcg ccggcctgga
     5221 cctcgagccc cgaccagtcg atcgtgcggc cgtcgaggcg gggcggcgtg ccggtcttga
     5281 ggcgggcgag ccggaagccg aggcgggcga gcgttcgggc gaggcctacc gctggtgcct
     5341 cgccgacccg cccggccgcg acccgctcgt cgccgatatg gatcacgccg cagaggaagg
     5401 tgccggtggt cagcaccacg cgcgcggcgc ggagcgcctc gcccgccgcg gtgaccacgc
     5461 cggcgaccgc gccgtcggca ccgagcacca ggtcctcgac cgccgcggcg cccaagcaga
     5521 ggccgtcctg cccggcaagc agcgcctgga cggcgccggc atagagcttg cggtcggcct
     5581 gcgcgcgcgg gccgcgcacc gccgggccct tgctggcgtt gagcacccgg aactggattc
     5641 cggcgcggtc ggcggcgagc cccatcagcc cgccaagcgc atcgacctcg cgcaccaggg
     5701 tgcccttggc gatgccgccg atggccgggt tgcacgacat cgcaccgatg gtgtcgaggc
     5761 ggtgggtgag gagcgcggtg cgcgcgccca cgcgcgccgc cgcggccgcc gcctcgcagc
     5821 cggcgtggcc gccgccgacc acgatcacgt catagctgtc gccgctgcgc atggcgcgaa
     5881 atctagggcc gggccgggcg cgagtcaaag ccgcgcgggc cgggatgttt cacgtgaaac
     5941 atcgcccccg actgtctact ttccgagaca gaactcggcg aagatcacgt cgaggatgtc
     6001 ctcgacgtcg acccggccgg tgatccggcc gagcgcggtg accgcgtggc gcaggtcctc
     6061 ggcggcgagc tcggccgcgt ccgctccttg cgcgcgggcc agcgcctcgg cacacgcctg
     6121 caacgccgcg cgatggcggg cgcgggtgat cggcgggccg tcgccgctgc ccgcctcgca
     6181 gcgtccggcg atcgccgcca ccaggccggc gatgccggcg ccggtcttgc acgaggtggc
     6241 gagcgccggc cggccgcaga ccgagcccgg tagcgctccc gcaaccaggt cggtcttgtt
     6301 gagcaccacc agggagttgc gggcgagcag agcgcgcgcc tgcgcgtccg gatcgatcgg
     6361 tccgctcgcg tcgagcacca gcaccaccag gtcggcggtc tcggcgcggg cgcgggcacg
     6421 ccgcaccccc tcgcgctcga cctcgccgcc ggcctcgcgc aggccggcgg tgtcggccac
     6481 cgtcaccggc accccggcga ggtcgagatg aacctcgatg acgtcgcgcg tggtacccgc
     6541 gcgggccgag acgatggcca cgtcgcgctg tgctaactgg ttgagaatac ttgattttcc
     6601 ggcgttcggc gggcccacga tggcaacctc cgcgccgtcg cgcaggcgct cgccgcggcg
     6661 gccgtcgtcg agatggccgg cgatctccgc cgccaccgcc tgcgcctcgg ccagcgccgc
     6721 cgccgccagg cccccgggca gcggctcgtc ggcgaagtcg agcccggcct cgaggtgggc
     6781 gagggtgcgc accaggcgct cgcgccagcc gtcatagagc cgccccagag cgccgtcgag
     6841 ctggcgcaaa gcctggcggc gctggccctc ggtctcggcg gcgaccaggt cggcgaggcc
     6901 ctcggccgcg gtcaggtcga gcttgccgtt gacgaacgcg cgccggctga actccccggc
     6961 ctcggccggg cgcaggccgg ggagcgcggc cagcgcctcg agcacgccgg cgaccaccgc
     7021 ccggccgccg tgaaggtgca actcggccac gtcctcgccg gtgaagctcg ccgggcccgg
     7081 gaaccacagc accagcgcg
//
LOCUS       DASWHU010000159         7839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_68796, whole genome shotgun sequence.
ACCESSION   DASWHU010000159 DASWHU010000000
VERSION     DASWHU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68796"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2781
                     /locus_tag="VGB88_06035"
     CDS             <1..2781
                     /locus_tag="VGB88_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEX9647037.1"
                     /translation="WHRFYSHQPDLNFDNPRVLEEVTKVMHFWLETGVDGLRLDAVPY
                     LVEREGTNNENLPESHAIVKRIRAELDARYPDRMLLAEANQWPEDTQEYFGTGDECHM
                     AFHFPLMPRMYMAIAQEDRHPITDIMRQTPEIPDLCQWAVFLRNHDELTLEMVTDEER
                     DYLWRTYASDPRARLNLGIRRRLAPLMDNDRPKIELMNGLLFSMPGTPIIYYGDEIGM
                     GDNVFLGDRDGVRTPMQWSPDRNAGFSRADPARLYLPPIMDPIYGYEAVNVEAQENDR
                     SSLLNWMRRIIAVRQGHRAFGRGRLRFLYPGNRKILAYLRQHEGETVLCVANLSRAAQ
                     PVELDLSEFRGRVPVELGGRTAFPPIGELPYLLTLGGYGFYWFELTEQAAQPTWHEET
                     PQPLPEFVTLVARDGWRSLVEGREARALETEVLPRYLVNQRWFAGKASDIEHCRLVEL
                     ATLADGGDGYLLAVAEATVAGEALSHFLPLATTWAEGATAWNSPLLPFCLAKIRRGAK
                     VGAVYDVVADDGFARALIAALADGAVIAGPSATIRFTPGARFASLRVGPETEIARIAK
                     EQTNSSLRIGDQAILKIYRRLQAGTQPEIEMGRYLTDLVAFDRCAPLLGCVESVPEGG
                     GAATALAVVHGYVANQGDGWTYTVEYLERWLEEALQLREAETTDGLAERHAGYLHVVR
                     TLARRTGEMHVALAGARAPKAFAAAPVGAADLRRWRAGTMRQARTAFAALRRALKRLD
                     DDARALAARVLAEREACLERIRDLTEGPIKAVRCRVHGDYHLGQVVLVHEDFYVLDFE
                     GEPARPFAERRQKTSPLKDVAGMLRSFDYAARTSARAVVPPAPEAAVRARALVAAWCE
                     LAQSSFLDAYRQAVAGSGAIPVNGRQAKRLLDLFMLEKALYEVAYEAANRPDWLSVPL
                     SGLHRMLGGDDG"
     gene            2774..5032
                     /gene="glgB"
                     /locus_tag="VGB88_06040"
     CDS             2774..5032
                     /gene="glgB"
                     /locus_tag="VGB88_06040"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX9647038.1"
                     /translation="MAEARKSAPGRQPPGARSLASLHAPLAAAIAALVHGDHGDPFAL
                     LGAHAHDGGAVVRAFLPEAAEAWVVDRRTGQQLAAMTRLHDAGFYAALVAGRRTVDYR
                     LRMRVGDEVSEVEDAYRFPMVLGELDLHLIGEGRHLELYRRLGAHPMAIGGVAGVAFA
                     VWAPNARRVSVVGEFNFWDGRRHPMRNRIEGGVWELFVPGVRKGARYKYEIKAADGAL
                     MPLKADPVGFHTERRPATASIVHGLPDHAWGDGEWLRGRAARARRDAPVSIYECHLGS
                     WMRVPEEGNRWLSYGELAERLIPYVKELGFTHLELLPVSEHPFDGSWGYQPAGLYAPT
                     SRFGAPEDFAALVDACHRAGLGVILDWVPGHFPTDPHGLGAFDGSHLYEHADPRQGFQ
                     LDWNTLLYNYGRREVANYLLANGLFWTDIYHCDGLRVDAVASMLYLDYSRRPGEWVPN
                     IHGGRENLEAIEFVKQTNAAIAARAPGVVTVAEESTAWPGVTHAQDSGGLGFSYKWNM
                     GWMHDTLSYVARDPAYRKYHHHDLTFGLLYAFSEDFVLPLSHDEVVHGKGSLLGRMPG
                     DRWQKFANLRAYLGFMFAHPGKKLLFMGGEFGQEREWDHDSSLDWHLLDDPLHKGVQR
                     LVRDLNHAYRSLPALHQLDGDSAGFEWLVGDDADNSVLAFLRRGREATAPVLAACNFT
                     PVPRFGYRIGVPAAGVWTEVVNTDAEAYGGGNLGNLGAAATEAVPAHGRAQSVSLTLP
                     PLAALILVQRPG"
     gene            5043..7187
                     /gene="glgX"
                     /locus_tag="VGB88_06045"
     CDS             5043..7187
                     /gene="glgX"
                     /locus_tag="VGB88_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX9647039.1"
                     /translation="MATHPAPRPGHPHPLGATWDGHGVNFALFSAHAETVELCLFDPA
                     GRREIERWTLPECSGQVWHGYLPDARPGQLYGYRVHGPYDPERGHRFNPNKLLIDPYA
                     KALDGTLRWTDAHFGYRAGSPRADLSFDRRDNARGVPKSRVVETAFTWGRERAPGTAL
                     ADTIVYELHVRGQTMLHPSLPKPLHGTCAGLTTPEVVRHLCDLGVTAVELMPVAAIID
                     ERRLVEQGLRNYWGYNSIAFFAPDPRYLATGRVEEFKTMVKVLHDAGIEVLLDVVYNH
                     TGEGNELGPTLCFRGIDNASYYRLAPGAPRGYVDDTGCGNTLNLEHPRVLQLVLDSLR
                     YWVTDMHVDGFRFDLAAALGRRQGAFDAHAAFFAAVGQDPVLARAKLIAEPWDLGEDG
                     YQLGNFPPPWSEWNDRYRDGVRRFWRGDEDVLPELGRRLMGSAELFERHGRRPTTSLN
                     FITAHDGMTLHDLVSYAQKHNDANGEANNDGHAEDFSNNYGVEGPTEDAAIRALRERQ
                     KRNLLATLLLSQGVPMLLAGDELGHSQRGNNNAYCQDNHTTWIDWQAVDADGLALCRF
                     VKKLVALRKAHPVLRRQHYLHGKARDQAGLEDVTWINRDGERASHQDWHDSMRRSIGL
                     VLNGQAGVDRAADGTPLADGILLILINGRDGTAEWTLPALDRGSAWRRLLCTADPEPA
                     AEPKPSAGSRIEVPARCVQVYLLDAAAGQAGG"
     gene            7230..7532
                     /locus_tag="VGB88_06050"
     CDS             7230..7532
                     /locus_tag="VGB88_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647040.1"
                     /translation="MIENDANEARYLARDERGRPRHYVAQWNDDLHHAAHVAVTGETG
                     GHNADHAESPVARLARSLADGYVYQGEPSAFRGGAGRGTLPARGCAWFLGEDNSEP"
     gene            7529..>7839
                     /locus_tag="VGB88_06055"
     CDS             7529..>7839
                     /locus_tag="VGB88_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017179228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9647041.1"
                     /translation="MTASAPLDRLAALHRISASYRDMAGRTHATSAETKRALLAAMGV
                     AAASDAEARASLAAAREAAPILPAGLVVGCEAPARIPLRLPPRTVGADVRWRLRCEDG
                     TV"
BASE COUNT         1134 a         2828 c         2744 g         1133 t
ORIGIN      
        1 tggcaccgct tctactccca ccagccggat ctgaatttcg acaatccgcg cgtgctggaa
       61 gaggtgacga aggtcatgca tttctggctg gagacgggcg tggacggctt gcgcctcgat
      121 gcagtgccct acctcgtcga gcgcgaaggc accaacaacg agaacttgcc cgagagccac
      181 gcgatcgtga agcgcatccg ggccgagctc gatgcccgct atcccgaccg catgctgctg
      241 gccgaggcca accagtggcc ggaggacacc caggagtatt tcggcaccgg cgacgaatgc
      301 cacatggcgt tccacttccc gctgatgccg cgcatgtaca tggcgatcgc ccaggaagac
      361 cgccatccga tcaccgacat catgcgccag acgcccgaga taccggacct gtgccaatgg
      421 gcggtcttcc tgcgcaatca cgacgagctg acgctcgaga tggtgaccga cgaggagcgc
      481 gactatctgt ggcgcaccta cgccagcgac ccgcgggcac ggctcaatct cggcatccgc
      541 cgccggctgg cgccgctgat ggacaacgac cggcccaaga tcgagctgat gaacggcctc
      601 ctgttctcga tgcccggcac gccgatcatc tattacggcg acgagatcgg catgggcgac
      661 aacgtctttc tcggcgaccg cgacggcgtg cgcacgccga tgcaatggtc gccggaccgc
      721 aacgccggat tctcgcgcgc cgaccccgcc aggctctatc tgccgccgat catggacccg
      781 atctacggct acgaggcggt caacgtcgag gcccaggaga acgatcgctc gtcgctgctc
      841 aactggatgc gccggatcat cgccgtccgc cagggccatc gcgccttcgg ccgcggccgg
      901 ctgcgcttcc tctatccggg caaccgcaag atcctcgctt acttgcgcca gcacgagggc
      961 gagaccgtcc tgtgcgttgc caacctttcg cgcgccgcgc agccggtcga gctcgacctg
     1021 tcggagtttc gcggccgggt gccggtcgag ctcggcgggc gcaccgcctt cccgccgatc
     1081 ggcgagcttc cctacctgct caccctcggc ggctacggct tctattggtt cgagctgacc
     1141 gagcaggcgg cgcagccgac ctggcacgag gagacgccgc agccgctgcc cgagttcgtc
     1201 accctggtcg cgcgcgacgg ctggcggagc ctggtcgaag gccgcgaggc gcgcgcgctc
     1261 gagaccgagg tgctgccgcg ctacctggtc aaccagcgct ggttcgcggg caaggcaagc
     1321 gacatcgagc actgccggct ggtcgagctc gccaccttgg ccgacggcgg cgacggctat
     1381 ctgctggcgg tggccgaggc caccgtggcg ggcgaggcgc tcagccactt cctgccgctg
     1441 gcaacgacct gggccgaggg cgcgaccgcc tggaactcgc cgctgctgcc gttctgcctg
     1501 gccaagatca ggcgcggcgc caaggtcggc gcggtctacg acgtggtcgc cgacgacggc
     1561 ttcgcccgcg cgctgatcgc cgcgctagca gacggggcgg taatcgccgg cccgtcggca
     1621 acgatacggt tcacgccggg cgcgcgcttc gcgagcttgc gcgtcggtcc cgagaccgag
     1681 atcgcgcgca tcgccaagga gcagaccaac tcgtcgctgc gcatcggcga ccaggcgatc
     1741 ctcaagatct atcggcgcct gcaggcgggc acccagcccg agatcgagat gggccggtac
     1801 cttaccgact tggtcgcctt cgaccgctgc gcgccgctgc tcggctgcgt cgagtcggtg
     1861 cccgaaggcg gcggcgcagc gacggcgttg gcggtcgttc acggctacgt cgccaaccag
     1921 ggcgacggct ggacctacac ggtcgagtac ctcgagcgct ggctcgagga ggcgctgcag
     1981 ttgcgcgagg cggagaccac ggacgggctc gccgagcggc acgcgggcta cctccacgtg
     2041 gtgcggacgt tggcgcggcg cacgggcgag atgcatgtcg cgctggccgg cgcgcgcgcg
     2101 cccaaggcgt tcgccgccgc gccggtcggc gccgccgatc tccgccgttg gcgcgccggc
     2161 acgatgcgcc aggcgcgcac cgcgttcgcc gcgctgcggc gcgcgctcaa gcgtctcgac
     2221 gacgacgccc gggcgctggc ggcgcgcgtg ctggctgagc gcgaagcgtg cctcgagcgc
     2281 atccgcgacc tcaccgaggg gccgatcaag gcggtgcgct gccgggtcca cggcgactat
     2341 cacctcggcc aggtggtgct cgttcacgag gacttctacg ttctcgactt cgagggcgag
     2401 ccggcgcgcc cgttcgccga gcgccggcag aagacctcgc cgctgaagga cgtggccggc
     2461 atgttgcgct cgttcgacta tgccgcgcgc accagcgccc gcgcggtggt gccgcccgcg
     2521 ccggaagcgg cggtgcgcgc gcgcgcgctg gtcgcggcgt ggtgcgagct ggcgcaatcg
     2581 agcttcctcg acgcctaccg gcaggcggtc gccggctccg gcgcgattcc cgtgaacggc
     2641 cgccaagcca aacgcctgct cgacctgttc atgctcgaga aggcgctcta cgaggtcgcc
     2701 tacgaggccg ccaaccgccc ggactggctg tcggtcccgc tgtccgggct gcaccgaatg
     2761 ctcggcggcg acgatggctg aggcgcgcaa gtccgcgcca gggcggcagc cgccgggcgc
     2821 gcgctcgctc gcgtccctgc acgcgccgct cgccgccgcg atcgccgcgc tcgtccacgg
     2881 cgaccacggc gacccgttcg cgctgctcgg cgcgcacgcg cacgacggcg gggctgtggt
     2941 gcgtgcgttc ctgcccgagg ccgccgaggc ctgggtcgtc gaccggcgga ccggacagca
     3001 gcttgccgcc atgacccggc tgcacgacgc cggattctac gccgcgctgg tggcggggcg
     3061 acgcaccgtc gattaccggc tgcgcatgcg cgtcggcgat gaggtgagcg aggtcgagga
     3121 cgcctatcgc ttccccatgg tgctgggcga gctcgacctg cacctgatcg gcgaagggcg
     3181 ccacctcgag ctctatcgcc ggctcggcgc ccacccgatg gcgatcgggg gcgtcgccgg
     3241 ggtggcgttc gcggtgtggg cgccgaacgc gcggcgggtc agcgtggtcg gcgagttcaa
     3301 cttctgggac ggccgccgcc acccgatgcg caaccgcatc gagggcggcg tgtgggagct
     3361 gttcgtgccc ggggtgcgca agggcgcgcg ctacaaatac gagatcaagg cggcggacgg
     3421 ggcgctgatg ccgctcaagg ccgacccggt cgggttccat accgagcggc gcccggcgac
     3481 cgcgtcgatc gtgcacggcc tccccgacca cgcctggggc gacggcgagt ggttgcgcgg
     3541 tcgcgcggcg cgcgcgcgcc gcgatgcgcc ggtgtcgatc tacgaatgcc atctcggatc
     3601 gtggatgcgg gtgcccgagg agggcaaccg gtggctgagc tatggcgagc tcgccgagcg
     3661 gctgatcccc tatgtcaagg agctcggctt tacccatctc gagctgttgc cggtgtccga
     3721 gcacccgttc gacggctcgt ggggatacca gcccgcgggc ctgtacgcgc cgaccagccg
     3781 gttcggggcg cccgaggact tcgccgcctt ggtcgatgcc tgccatcgcg ccgggctcgg
     3841 cgtcatcctc gactgggtgc cggggcattt cccgaccgac ccgcacggcc tcggcgcgtt
     3901 cgacggcagc catctgtacg agcacgccga cccgcgccag ggcttccagc tcgactggaa
     3961 caccctgctg tacaattacg gccgccgcga ggtggccaat tatctgctgg ccaatggcct
     4021 gttctggacc gacatctacc attgcgacgg cttgcgcgtc gacgccgtgg cctcgatgct
     4081 ctatctcgac tacagccggc ggcctgggga gtgggtgccc aacatccacg gcgggcgcga
     4141 gaacctggag gcgatcgagt tcgtcaagca gaccaacgcc gcgatcgccg cgcgcgcgcc
     4201 gggcgtggtc acggtggccg aggagtcgac cgcctggcca ggcgtcacgc acgcgcaaga
     4261 ctccggcggc ctcggcttct cctacaagtg gaacatgggc tggatgcacg acacgctgtc
     4321 ctacgtcgcg cgcgatccgg cctaccgcaa gtatcaccat cacgacctca ccttcggcct
     4381 gctctacgcc ttcagcgagg acttcgtgct gccgctcagc cacgacgagg tggtccacgg
     4441 caagggctcg ctgctcgggc ggatgcccgg cgaccgctgg cagaagttcg ccaacctgcg
     4501 cgcctatctc ggcttcatgt tcgcccatcc gggcaagaag ctgttgttca tgggcggcga
     4561 gttcggccag gagcgcgagt gggaccacga ttcgagcctc gactggcacc tgctcgacga
     4621 cccgctgcac aagggcgtcc aaaggctggt ccgcgacctc aatcatgcct accggtcgct
     4681 gcccgcgctg caccagctgg acggcgactc ggcggggttc gagtggctgg tcggcgacga
     4741 cgccgacaac agcgtgctcg cgttcctgcg ccgcgggcgg gaggccacgg cgccggtgct
     4801 ggccgcgtgc aacttcaccc cggtgccacg tttcggatat cggatcggcg tgccggcggc
     4861 cggggtctgg accgaggtcg tcaacaccga cgccgaggcc tatggcggtg gcaacctcgg
     4921 caatctcggc gccgccgcca ccgaagccgt gccggcgcac ggccgcgccc agtcggtgtc
     4981 gctgaccttg ccgccgctcg ccgcgctgat cctggtccag cggcccgggt gatcctgccg
     5041 ccatggccac ccatcccgcc ccgcggcccg ggcaccccca ccccctcggc gcgacctggg
     5101 atgggcacgg ggtcaacttc gccctgttct cggcccatgc cgagacggtc gagctgtgcc
     5161 tgttcgaccc ggccgggcgg cgcgagatag agcgctggac tctgcccgaa tgcagcggcc
     5221 aggtctggca cggttatctg cccgacgccc gccccggcca gctttacggc taccgggtgc
     5281 acggccccta cgaccccgag cgcggccatc gcttcaaccc caacaagctc ttgatcgacc
     5341 cgtatgccaa ggcgctcgac ggcactctcc gctggaccga cgcccatttc ggctatcgcg
     5401 ccggcagccc gcgcgccgac ctgtccttcg accgccgcga caacgcccgc ggcgtgccca
     5461 agtcgcgggt ggtggagacc gcgttcacct ggggccggga gcgcgcgccg ggcaccgcgc
     5521 ttgccgacac gatcgtctac gagctgcatg tgcgcggcca gaccatgctg catccgtcgc
     5581 tgccgaagcc gcttcacggc acctgcgccg ggctgaccac gcccgaggtg gtccgccacc
     5641 tttgcgacct cggcgtgacc gctgtcgagc tgatgccggt cgccgcgatc atcgacgagc
     5701 gccggctggt cgaacagggc ctgcgcaact attggggcta caacagcatc gccttcttcg
     5761 cccccgaccc gcgctatctc gccaccggcc gggtcgagga gttcaagacc atggtcaagg
     5821 tgctgcacga cgccggcatc gaggtgctgc tcgacgtggt ctacaaccac accggggagg
     5881 gcaacgagct gggaccgacg ctgtgctttc gcggcatcga caacgcgagc tactaccggc
     5941 tcgcgcccgg cgcgccgcgc ggctatgtcg acgacaccgg ctgcggcaac acgctcaacc
     6001 tcgagcatcc gcgggtcctc cagctggtgc tcgattcgct gcgctactgg gtcaccgaca
     6061 tgcatgtcga cggcttccgc ttcgacctcg ccgccgcgct cgggcggcgc cagggcgcgt
     6121 tcgatgcgca cgccgcgttc ttcgccgccg tcggccagga cccggtgctg gcccgtgcca
     6181 agctgatcgc cgagccgtgg gacctgggcg aggacggcta tcagctcggc aacttcccgc
     6241 cgccgtggtc ggagtggaac gaccgctacc gcgacggcgt gcgccggttc tggcgcggcg
     6301 atgaggacgt gctgcccgag ctcgggcgcc ggctcatggg ctcggccgag ctgttcgagc
     6361 gtcacggccg ccggcccaca acctcactca acttcatcac cgcccacgac ggcatgacct
     6421 tgcacgacct ggtgtcgtat gcgcagaagc acaacgacgc caacggcgaa gcgaacaacg
     6481 acggccacgc cgaggacttc agcaataact acggcgtcga ggggccgacc gaggatgccg
     6541 cgatccgcgc gctgcgcgag cgccagaagc gcaacctgct ggcgaccctg ctgctgtcgc
     6601 agggcgtgcc gatgctgctt gcgggcgacg agctcggcca cagccagcgc ggcaacaaca
     6661 acgcctattg ccaggacaac cataccacct ggatcgactg gcaggcggtc gacgccgacg
     6721 gcctggcgct gtgccgcttc gtcaagaagc tggtggcgct acgtaaggca catccggtgc
     6781 tgcgccggca gcactacctg cacggcaagg cgcgcgacca ggcggggctc gaggacgtca
     6841 cgtggatcaa ccgcgacggc gagcgggcga gccaccagga ttggcatgat tccatgcgcc
     6901 ggtcgatcgg cctggtgctc aacgggcagg cgggggtgga ccgggcggcg gacggaacgc
     6961 cgctcgccga cggcatcctg ttgatcctga tcaatggccg ggacggcacg gcggagtgga
     7021 ccttgcctgc gctcgaccgg ggctcggcgt ggcgccggct gctgtgcacc gccgatcccg
     7081 agcccgcggc ggagcccaag ccgtccgcag gcagccgcat agaggtgccc gcgcgctgcg
     7141 tgcaggtcta cctgctcgac gcggccgccg ggcaggcggg agggtgagcg gccggcgcgg
     7201 tcgcgggccc tgagcggcac gtccacctgg tgatcgagaa cgacgccaat gaggcgcgct
     7261 acctggcgcg cgacgagcgc ggccgcccgc gccattatgt cgcgcagtgg aacgacgacc
     7321 tgcatcacgc cgcccatgtt gcggtcaccg gcgagaccgg cggccacaac gccgaccatg
     7381 cggagtcgcc ggtggcgcgg ctcgcgcgat cgctggcaga cggctacgtc taccagggcg
     7441 agccgtcggc atttcgcggc ggcgccggcc gcggcacgct gccggcgcgg ggctgcgcct
     7501 ggttccttgg cgaggataac agcgagccgt gaccgcgtcg gcgcccctcg accgcctcgc
     7561 cgcgctgcac cggatctccg cgtcctatcg cgacatggcc ggccgcacgc acgcaacgag
     7621 cgcggagacc aagcgggcgc tgctcgccgc aatgggcgtc gccgcggcga gcgacgccga
     7681 ggcccgggca tcgctcgccg cggcgcggga ggcggcgccg atcctgcccg cgggcctggt
     7741 ggtcggctgc gaggcgccgg cgcgcattcc gctgcgcctg ccgccgcgga ctgtcggggc
     7801 ggatgtgcgc tggcggctcc gctgtgagga cggcacggt
//
LOCUS       DASWHU010000160         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1934139, whole genome shotgun sequence.
ACCESSION   DASWHU010000160 DASWHU010000000
VERSION     DASWHU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934139"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..568)
                     /gene="infC"
                     /locus_tag="VGB88_06060"
     CDS             complement(32..568)
                     /gene="infC"
                     /locus_tag="VGB88_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9647042.1"
                     /translation="MARFKAPLPPQREGPRANDQITADPVRLVDQNGNMVGVVPLREA
                     LEQAGNVNLDLVEVSPNAEPPVCKVLDYGKFKYDAQKRAAEARKKQKTIEIKEIKMRP
                     GIDAHDYEVKMRSITKFLGDGDKVKVTLRFRGRELAHQDIGLRVLERVKGELEEIGKV
                     EQFPKMEGRQMTMVLAPR"
     gene            complement(682..2640)
                     /gene="thrS"
                     /locus_tag="VGB88_06065"
     CDS             complement(682..2640)
                     /gene="thrS"
                     /locus_tag="VGB88_06065"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9647043.1"
                     /translation="MPASAPAPETVAITLPDDSVRRYPRPVTAAEVAADIGPGLAKAA
                     LVARVDGELWDLKRPIEGDVHVSIVTARDEAGLEVIRHDAAHVLAEAAKELWPDIQVT
                     IGPVIADGFYYDFARAEPFTPDDLVTLEARMRDIVARDEPVTREVWDRHDAIAYFEGV
                     GERYKAQIIRDLPEDEEITIYRQGGFTDLCRGPHLPSTGKLGTAFKLLKIAGAYWRGD
                     SRNEMLQRVYGTAWADDKALAAYLHRLEEAERRDHRRLGREMGLYHFQEEAAGSVFWH
                     PKGWVLYRVVQDYMRRKLDRAGYVEVRTPQLLDRSLWERSGHWEKFREHMFTAEAEDR
                     VLALKPMSCPCHVQIFRQGLKSYRDLPLRMAEFGSCHRNEPSGALHGLMRVRAFVQDD
                     AHIFCTEDQITDETVRFCTLLMGIYRDFGFAEVKVNFADRPGVRAGADAVWDRAEAAL
                     LDACRATEIEVGTNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFVLPERLDASYVG
                     EDGAKHRPVMLHRAILGSFERFLGILIEHYAGRLPMWLAPVQAVVATITSDGDGWART
                     VHARLLAAGLRAELDIRNEKINYKVREHSLAKVPAILAIGRREAEEGTVSLRRLGGRD
                     QEVLALDEAATTLKNDAADPIARAGSAE"
     gene            complement(2682..3620)
                     /locus_tag="VGB88_06070"
     CDS             complement(2682..3620)
                     /locus_tag="VGB88_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9647044.1"
                     /translation="MAAERILVIKLGAMGDWVLATGPFAAIRAHHRDAEITLLTLPEF
                     AGWGGRCGWFDRVWTDDRPGWLRPGAWLALGRRLARAGFARVYDLQTSDRSSLCFRLW
                     PRPRPEWSGIARGCSHPHANPRRDLMHTVERQAEQLAMAGIAQVPPPSLDWLDGDLAG
                     LDVPARAALLVPGGSAHRPGKRWPADSYAALARGLGARGVTPLLIGAGPEAEVLARIA
                     AAAPGARDLGGRLGLDQLAALARRAEVAVGNDTGPMHVIATAGCPSVVLFSGDSDPAL
                     CAPRGPRVRVLRRQPLAELGVAEVAEVALATARGSA"
     gene            complement(3610..>4421)
                     /locus_tag="VGB88_06075"
     CDS             complement(3610..>4421)
                     /locus_tag="VGB88_06075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9647045.1"
                     /translation="GTYGHSGALKRRYNAVMAAGEVVIAISEHISRHLTAVYGTDPAR
                     IRVIHRGVDLGRFDPAAVAPERVAALRAAWGIAGESRVVLLPGRLTRWKGQAVLVEAM
                     ARTARADVACVLVGDAQGRERYRAELERAIRRHGLGDRVRLAGACADMPAAYLAADLV
                     VSASTDPEAFGRIAVEAQAMARPVIATAHGAALETVADGETGWLVPPGDAEALAAALA
                     RALALDPAARAAIGRAGRARAARLFTTEAMCAKTLAVYRSLLEVREARGGG"
BASE COUNT          608 a         1627 c         1565 g          621 t
ORIGIN      
        1 gatggatgac catgtttcaa atcatcaggc gctagcgcgg cgccagcacc atggtcatct
       61 ggcggccctc catcttcggg aactgctcga ccttgccgat ctcctcgagc tcgcccttga
      121 cccgctcgag cacgcgcagg ccgatgtcct gatgcgccag ctcgcggccg cggaagcgca
      181 aggtcacctt gaccttgtcg ccgtcgccga ggaacttggt gatgctccgc atcttgacct
      241 cgtagtcatg cgcatcgatg cccggacgca tcttgatctc cttgatctcg atggtcttct
      301 gcttcttgcg cgcctcggcg gcgcgtttct gggcatcgta cttgaacttg ccgtagtcga
      361 ggaccttgca caccggcggc tcggcgttgg gcgatacctc gaccaggtcg aggttcacat
      421 tccctgcctg ctccagcgcc tccctcagcg gcacgacacc gaccatgttg ccgttctggt
      481 cgaccaggcg caccggatca gcggtgatct ggtcgttcgc gcgcggcccc tcgcgttgcg
      541 ggggcagcgg ggccttgaat ctggctatct atccgactcc cttgtcgttg caccaggcgc
      601 cgccgcgcgg acgcggcggt acggacggcg gcccccatga ttccgccgtc tcaacctgtc
      661 catgacagcc ggggagccac ctcattctgc gctgccggcc cgcgcgatcg ggtcggcggc
      721 atcgttctta agtgtagttg cggcttcgtc gagcgcaagt acttcctgat cgcgcccgcc
      781 gagccggcgc agcgagacgg tgccctcctc ggcctcgcgc cggccgatcg ccaggatcgc
      841 cggcaccttg gccaggctgt gctcgcgcac cttgtagttg atcttctcgt tgcgaatgtc
      901 gagctcggcg cgcagccccg cggccagcag acgcgcgtgc accgtgcgcg cccacccgtc
      961 gccgtccgag gtgatcgtcg ccaccaccgc ctggaccggc gccagccaca tcggcagccg
     1021 gccggcataa tgctcgatca ggatgccgag gaagcgctcg aacgagccca ggatcgcgcg
     1081 gtgcagcatc accgggcgat gcttggcgcc gtcctcgccg acgtacgagg catcgagccg
     1141 ctcgggcagc acgaaatcga cctggagggt gccgcactgc cagtcgcggc cgatcgcatc
     1201 gcgcagcacg aactcgagct tcgggccgta gaacgcgccc tcgcccgggt tggtgccgac
     1261 ctcgatctcg gtcgcccggc aggcgtccaa gagcgccgcc tcggcgcggt cccagaccgc
     1321 atccgcgccc gcgcgcaccc ccggccggtc ggcgaagttg accttcactt cggcgaagcc
     1381 gaagtcgcgg taaatgccca tcagcagcgt gcagaagcgc accgtctcgt cggtgatctg
     1441 gtcctcggtg cagaagatgt gggcgtcgtc ctggacgaag gcgcgcaccc gcatcagccc
     1501 gtggagcgcc cccgacggct cgttgcggtg gcaggagccg aactcggcca tgcgcagcgg
     1561 caggtcgcgg tagctcttca agccctggcg gaagatctgc acgtggcacg ggcagctcat
     1621 cggcttgagc gccagcaccc ggtcctcggc ctcggcggtg aacatgtgct cgcggaattt
     1681 ctcccagtgg cccgagcgct cccacagcga gcggtcgagc agctggggcg tgcgcacctc
     1741 gacatagccg gcacggtcga gcttgcgccg catgtagtcc tggaccaccc ggtacaaaac
     1801 ccaccccttg gggtgccaga acacgctgcc ggcggcctcc tcctggaagt ggtagaggcc
     1861 catctcgcgc ccgagccggc ggtggtcgcg cctctcggcc tcctccagac ggtgcaggta
     1921 ggcggcgagc gccttgtcgt cggcccaggc ggtgccgtag acgcgctgca gcatctcgtt
     1981 gcgggagtcg ccccgccagt aggcgccggc gatcttgagc agcttgaacg cggtgccgag
     2041 cttgccagtc gagggcaaat gcggcccgcg gcagagatcg gtgaagccgc cttggcggta
     2101 gatcgtgatc tcctcgtcct cgggcaggtc gcggatgatc tgggccttgt agcgttcgcc
     2161 gacgccctcg aagtacgcga tcgcgtcgtg ccggtcccag acctcgcgcg tgaccggctc
     2221 gtcgcgggcg acgatgtcgc gcatccgcgc ctccaaggtg acgaggtcgt ccggggtaaa
     2281 cggctcggcg cgggcgaagt cgtagtagaa gccgtcggcg atcaccggcc cgatggtcac
     2341 ctggatgtcg ggccacagct ccttggcggc ctccgccagc acgtgggcgg cgtcgtggcg
     2401 gatgacctcg agcccggcct cgtcgcgggc ggtgacgatg ctgacatgga cgtcgccctc
     2461 gatcggccgc ttgaggtccc acagctcgcc gtcgacccgc gcgaccagcg ccgccttggc
     2521 cagccccggg ccgatgtcgg ccgcgacctc ggccgccgtc accggccgcg gatagcggcg
     2581 gaccgagtcg tcgggcagcg tgatcgccac cgtctcgggc gccggcgcgg atgcgggcat
     2641 ttccatgacg cgccggacaa tgggtggcgg gccgggagct gtcaagccga cccgcgggcc
     2701 gtcgccagcg ccacctcggc gacctccgcg accccgagct cggcgagcgg ttggcgcctg
     2761 agaacccgca cccggggccc gcgcggagcg cacagcgcgg ggtcggagtc gccactgaac
     2821 agcaccaccg acgggcagcc ggcggtggcg atcacgtgca tcgggccggt gtcgttgccg
     2881 accgcgacct cggcgcgccg cgcgagcgcg gcgagctgat cgaggccaag cctgccgccg
     2941 aggtcgcgcg cgccgggcgc ggcggcggcg attcgcgcca gcacctcggc ctcaggcccg
     3001 gcaccgatca gcagcggcgt gacgccgcgc gcgccaagcc cgcgcgcgag cgcggcgtag
     3061 gagtccgccg gccagcgctt gcccggccgg tgcgccgagc cgcccggcac cagcagcgcc
     3121 gcacgcgccg gcacatccag gccggcgagg tcgccgtcga gccagtcgag cgacggcggc
     3181 ggcacctggg cgatgccggc catggccagc tgctcggcct ggcgctcgac cgtgtgcatc
     3241 aggtcgcggc gcggattcgc gtgcgggtgc gagcagccgc gggcgattcc cgaccactcc
     3301 gggcgcgggc gcggccacag ccggaagcac aggctcgagc ggtcggaggt ctggaggtca
     3361 tagacgcgcg cgaagccggc gcgggcgagg cgccggccca gcgccagcca ggcgcccggc
     3421 cgcaaccagc ctggccggtc gtcggtccac acccggtcga accagccgca gcggccgccc
     3481 cagccggcga actcgggcag ggtcagcagg gtgatctcgg cgtcgcggtg atgggcgcgg
     3541 atggcggcga acggcccggt cgccagcacc cagtcgccca tcgcgccgag cttgatcacc
     3601 aggatgcgct cagccgccac cgcgcgcctc ccgcacctct aacagcgagc ggtagacggc
     3661 gagcgtcttg gcgcacatcg cctcggtggt gaacagccgc gcggcgcgcg cgcgtccggc
     3721 gcgcccgatc gccgcgcgcg ccgccggatc gagcgccagc gcgcgtgcga gtgccgccgc
     3781 cagcgcctcg gcgtcgcccg gcggcaccag ccagccggtc tcgccgtcgg cgacggtttc
     3841 gagcgccgcc ccatgggcgg tggcgatcac cggccgcgcc atcgcctggg cctcgaccgc
     3901 gatgcggccg aacgcctccg ggtcggtcga ggccgagacc accaggtcgg cggcgagata
     3961 ggcggcgggc atgtcggcac aggcgccggc gaggcgaacc cggtccccga ggccgtggcg
     4021 gcggatcgcc ctttcgagct cggcgcggta gcgctcgcgc ccctgggcgt cgccgaccag
     4081 cacgcaggcc acatcggcgc gcgccgtccg cgccatcgcc tcgaccagca ccgcctggcc
     4141 tttccagcgg gtgagccggc cgggcagcag cacgacgcgc gactcgccgg cgatgcccca
     4201 cgccgcgcgc agcgccgcga cgcgctcggg cgccaccgcg gccgggtcga agcggcccaa
     4261 gtcgacgccg cggtggatca cccggatgcg cgccgggtcg gtaccgtaga cggcggtgag
     4321 gtggcgggag atgtgctcgg agatcgcgat caccacctcg cccgccgcca tcaccgcgtt
     4381 gtagcgccgc ttgagagcgc ccgaatggcc gtaggtgccg t
//
LOCUS       DASWHU010000161         9913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3039458, whole genome shotgun sequence.
ACCESSION   DASWHU010000161 DASWHU010000000
VERSION     DASWHU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039458"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..77)
                     /locus_tag="VGB88_06080"
     CDS             complement(<1..77)
                     /locus_tag="VGB88_06080"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9647046.1"
                     /translation="MHLTPREKDKLLVSMAAMVARRRLER"
     gene            complement(96..974)
                     /locus_tag="VGB88_06085"
     CDS             complement(96..974)
                     /locus_tag="VGB88_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX9647047.1"
                     /translation="MYAVISPSEPAPARALRHTPRTSGVLDAVIERRAKGDVLAHVYA
                     CAPLRLMVPRVDADEPLTGIVVNTAGGIVGGDRLRAQVTLGAGSGALLIGQAAEKVYR
                     SAGDDATLACRYRLAAGAVLEVLPQGTILFDGARLRRVTEVGRHPTARLLAGEVLVLG
                     RIGMGETFARGLLHDRWVVACDSGARWIDALRLEADIARLHDAAAGLAGARALATVVY
                     AADDAPACQELARALLAAAPAGVRAAATVVAGVLLVRWLGADPAAVRAALGAFWSAFR
                     AHALGRPARLPVVWHR"
     gene            complement(949..1644)
                     /gene="urtE"
                     /locus_tag="VGB88_06090"
     CDS             complement(949..1644)
                     /gene="urtE"
                     /locus_tag="VGB88_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX9647048.1"
                     /translation="MLEVRDIDLAYGASRALRGVSLEAPIGRVTCVLGRNGVGKTSLL
                     RAVVGQHPVARGTILWDGEDVTGLPAHRRAARGIAYVPQGREIFPLLTVEENLRTGYA
                     ALKRAERSIPERVFELFPVLKTMLQRRGGDLSGGQQQQLALARALVMRPRLLVLDEPT
                     EGIQPSIIKDIERVIRALRETGTMAILLVEQYFEFARRLADSYAVLNRGEVVARGTTG
                     DLDHSDVRRHLTV"
     gene            complement(1648..2448)
                     /gene="urtD"
                     /locus_tag="VGB88_06095"
     CDS             complement(1648..2448)
                     /gene="urtD"
                     /locus_tag="VGB88_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX9647049.1"
                     /translation="MVDTALRVQPPEEVAAVAPRPGDTLLYLEGVTVSFDGFKALDDL
                     NFFAVARRMEAVIGPNGAGKTTMMDVISGRTRPDRGRIIFRDGIDLTRLDEAAIANLG
                     IGRKFQRPTVFQNHSVFDNLELALKGGRGVLASLFARLDGAQRDRIDEVLAIIALTDH
                     ARRPAGGLSHGQKQWLEIGMLLMQDPELLMVDEPVAGMTDAESEQTADLLRHIALTHS
                     VLVVEHDMDFVRALDARVTVMHEGAILAQGNLDAVQNDERVIEVYLGR"
     gene            complement(2441..3562)
                     /gene="urtC"
                     /locus_tag="VGB88_06100"
     CDS             complement(2441..3562)
                     /gene="urtC"
                     /locus_tag="VGB88_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX9647050.1"
                     /translation="MWSRIDLGGRLLLAALALAAVAVPAFNLSVPADSALHVPTYIVS
                     LLGKYLCFALLALSVDLVWGYCGILSLGHGAFFALGGYAMGMHLMRQIGTRGVYGHPE
                     LPDFMVFLNWTELPWYWHGFDGFAFAALMVVLVPGGLAFLFGWLAFRSRVTGVYLSII
                     TQALTFALMLAFFRNDMGFGGNNGLTDFKDLLGFDLQADGTRAGLFAASAAALALGYL
                     ACRALVTSRFGRVLVAVRDAESRTRFLGYRVEHYKLFAFVVSAVLAGIAGALYVPQAG
                     IINPGEFSPANSIEIVIWVAVGGRGTLFGAALGAVLVNYAKSTFTGALPEVWLYLLGA
                     LFIAVTLFLPKGVVGAVRSLRLALAGGARARPAQAAADG"
     gene            complement(3584..5224)
                     /gene="urtB"
                     /locus_tag="VGB88_06105"
     CDS             complement(3584..5224)
                     /gene="urtB"
                     /locus_tag="VGB88_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX9647051.1"
                     /translation="MVPIRRLVRVLVGGALLLGAGAACAEDIEALVTALAASGFDGKI
                     AAAEALAATGDERAGGALQAMLDGRLYLRTDDGAVVVADKAGGGYALTDPLSGAALGA
                     AEAKALDKIKVNNRLRGTLRAAIGRLTLMSPDRAKRMAAADAVFKKPDDSGLALLADA
                     LAAEADAAVRARLERALAAGRLALSKEPAARIAAVETLAGFTDSDSRALVASLLVARD
                     DGSFAEPDAEVRAAAAAALARIEARIERWQLLGSLFQGASLGSVLLLAAIGLAITFGV
                     MGVINMAHGEMVMLGAYTTFVVQELFRAYLPGAFGWSLAVAVPAAFVLVGAFGVAVER
                     GLIRFLYGRPLETLLATWGLSLVLQQFVRSVFGPTNREVGNPSWMTGAIDLAGAVVLT
                     YNRIWIIAFALAVFALLLLVIARTPFGLRMRAVTQNRAMAASMGIRTGMIDALTFGLG
                     CGVAGIAGVALSQIDNVSPNLGRGYIVDSFMVVVFGGVGNLWGTLVGALTLGVVNKFL
                     EPLAGAVLGKIFVLVAIILFIQKRPRGMFALRGRAAEA"
     gene            complement(5319..6614)
                     /gene="urtA"
                     /locus_tag="VGB88_06110"
     CDS             complement(5319..6614)
                     /gene="urtA"
                     /locus_tag="VGB88_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX9647052.1"
                     /translation="MTRITRMLGRACVAAAVAAAGFTAPAAVGADTIKIGVLHSLSGT
                     MAISETTLKDTILMLVAELNAQGGLLGKQVEAVVVDPASDWPLFAEKARELLQKDKVA
                     AVFGCWTSVSRKSVLPVFEELDGLLYYPVQYEGEESSRNVFYTGAAPNQQAIPAVEYL
                     MSEAGGGAQRWVLLGTDYVYPRTTNKILRAFLHARGVGDDDILETYTPFGHSDWQTIV
                     SEVAAFASQGKPTAVVSTINGDANVPFYKELANQGISAEDVPVVAFSVGEEELSGLDT
                     APLVGHLAAWNYFMSVDSPENAAFIDKWHAFIGNAKRVVNDPMEAHYIGFHMWAQAVA
                     QAGTTEVDAVRQAMYGQTVRSLSGYDVVMNTNHHLSKPVMIGEIQDDGQFDVVWQTDG
                     VVVGDAWSDFIPESAKLTADWTFPWVCGNCEAPLYKISY"
     gene            7113..7730
                     /locus_tag="VGB88_06115"
     CDS             7113..7730
                     /locus_tag="VGB88_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9647053.1"
                     /translation="MARTSKTTTTKPAAKTGAKPAARRKRAAPKAEVPAAARKAEVPA
                     VRSVFDQWLQLRNEVDRVFENFTAGFRFPGFASRMFDLEAWPLPRLLSQQLLMRTDLS
                     ETDKAVELRAELPGLTEADIDVTVSDDVLTVTGKKQEEREERGKEFHLRERSFGSFKR
                     SFRLPAGVDASKISAAFDKGVLTVTVPKPERPKAPRKKIAVARKA"
     gene            7758..8039
                     /locus_tag="VGB88_06120"
     CDS             7758..8039
                     /locus_tag="VGB88_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647054.1"
                     /translation="MGKQLEPLASVLDDMAEALREMSEMIYDNFSEATNARGVLRALI
                     LDGDPEEAGAELVRWAERRAGQPSNLDRAKDLADRIERLKGRINALAVD"
     gene            8076..9191
                     /locus_tag="VGB88_06125"
     CDS             8076..9191
                     /locus_tag="VGB88_06125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX9647055.1"
                     /translation="MSSADLVALLLCGDVMTGRGIDQILPRPSDPRLCESYAASAADY
                     VGLAERAHGPIPRPVAPAYVWGAALEALARARPDLGIVNLETAVTTSDDCVAKGVNYR
                     MHPANVACLAAAGIDCCVLANNHVLDWGRAGLVETLATLEGAGIKTAGAGRDRAAAAA
                     PAVLEAAGKGRVLVFAYGMAGSGVPPDWAAEADRPGINYLAEPSVDAAHRIAEAVSAV
                     RRPGDVVVVSIHWGGNWGYQVPEAQRRFAHRLIDAGAADVIHGHSSHHVKAIEVYRDR
                     LILYGCGDVLNDYEGIGGYQEYRGDLALMYLARIVVGTGELAGLALMPFRMRKFSLER
                     PPAADIAWLLEVIERECRRFGTRIAARDGYDLVLGWD"
     gene            9396..9776
                     /locus_tag="VGB88_06130"
     CDS             9396..9776
                     /locus_tag="VGB88_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647056.1"
                     /translation="MLLQVVHKPGTVPPTADEPGQELWAGGYQADRAEETDELTTSLA
                     IASEGQLEIGTWYHLSDLKAYLAPAKQDDTWTEIMVPVDRPDNRHNTHTLTQQVFAIN
                     TIINAIVALFEKLTPNEDPWIRVE"
BASE COUNT         1642 a         3487 c         3436 g         1348 t
ORIGIN      
        1 cgctcgagcc gccggcgcgc gaccatcgcg gccatcgaga ccagaagctt gtccttctcg
       61 cgtggtgtca ggtgcaccgt gtccctcccc ctcggtcaac gatgccagac caccggcagg
      121 cgcgccgggc ggccgagggc gtgggcgcgg aatgcgctcc agaacgcgcc gagcgcggcg
      181 cgcaccgccg ccgggtcggc gccgagccag cgcaccagca gcacgcccgc gaccacggtc
      241 gccgcggcgc gcacacccgc cggcgccgcg gccagcagcg cgcgggcgag ctcctggcac
      301 gccggcgcat cgtcggccgc atagaccacg gtggcgagcg cccgcgcgcc ggcgaggccg
      361 gccgcggcgt cgtgcaggcg cgcaatgtcg gcctccagcc tgagcgcgtc gatccagcgc
      421 gcaccactgt cgcacgcgac cacccagcgg tcgtgcagca ggccgcgggc gaaggtctcg
      481 cccatgccga tccgccccag caccagcacc tcgccggcga gcaggcgcgc ggtcggatgg
      541 cggccgacct cggtgacgcg cctgaggcgc gcgccgtcga acaggatggt gccttggggc
      601 aagacctcga gcaccgcgcc ggcggcgagg cggtagcggc aggcgagcgt ggcgtcgtcg
      661 ccggccgagc ggtagacctt ttccgccgcc tggccgatca gcagcgcgcc gctgccggcg
      721 ccgagcgtga cctgcgcgcg cagccggtcg ccgccgacga tgccgccggc ggtgttgacg
      781 acgatgccgg tcagcggctc gtcggcatcg acccgcggca ccatcaggcg cagcggcgcg
      841 caggcataga catgcgcgag cacgtcgccc ttcgcgcgcc gctcgatcac ggcatcgagc
      901 acgcccgagg ttcgcggcgt gtgccgcagc gcgcgcgccg gcgccggctc agacggtgag
      961 atgacggcgt acatccgaat ggtcgaggtc gcccgtggtg ccgcgcgcga ccacctcgcc
     1021 gcggttcagc accgcatagc tgtcggcgag ccggcgcgca aactcgaaat actgctcgac
     1081 cagcaggatc gccatggtgc cggtctcgcg caaggcgcgg atcacccgct cgatgtcctt
     1141 gatgatcgac ggctggatgc cctcggtcgg ctcgtcgagg accaacaggc gcgggcgcat
     1201 gaccagcgcg cgcgcgagcg ccagctgctg ctgctggccg cccgacaggt cgccgccgcg
     1261 ccgttgcagc atggtcttga gcaccgggaa cagctcgaac acgcgctccg ggatcgagcg
     1321 ctcggcgcgc ttgagcgccg cgtatccggt gcgcaggttc tcctcgaccg tgagcagggg
     1381 aaagatctcg cggccctgcg gcacgtaggc gatgccgcgc gcggcgcgcc ggtgcgcggg
     1441 cagcccggtc acgtcctcgc cgtcccacag gatggtgccg cgcgccaccg gatgctggcc
     1501 gaccacggcg cgcaacaggc tggtcttgcc gaccccgttg cggccgagca cgcaggtgac
     1561 ccggccgatg ggcgcttcca ggctcacccc gcgcagcgcc cggctcgcgc cataggcgag
     1621 gtcgatgtcg cgcacctcga gcatcgctca gcgccccaga tagacctcga tcacgcgctc
     1681 gtcgttctgg accgcgtcca ggttgccctg ggccaggatc gcgccctcgt gcatcacggt
     1741 gacgcgcgcg tccagcgcgc gcacgaagtc catgtcgtgc tcgaccacca gcaccgaatg
     1801 ggtcagggcg atgtgcctga gcaggtcggc ggtctgctcg ctctcggcat cggtcatgcc
     1861 cgccaccggc tcgtcgacca tcaacagctc gggatcctgc atcagcagca tgccgatctc
     1921 aagccactgc ttctggccgt gggacagccc gccggcggga cgccttgcgt ggtccgtgag
     1981 cgcgatgatg gcgagcacct cgtcgatgcg gtcgcgctgg gcgccgtcga ggcgggcgaa
     2041 cagcgacgcg agcacgccgc gcccgccctt gagcgcgagc tcgaggttgt cgaacacgct
     2101 gtggttctgg aacacggtcg ggcgctggaa cttgcgcccg atgccgaggt tggcgatcgc
     2161 ggcctcgtcg aggcgggtga ggtcgatgcc gtcgcgaaag atgatgcggc cgcgatcggg
     2221 acgggtgcgg ccgctgatca cgtccatcat cgtggtcttg ccggcgccgt tcggcccgat
     2281 caccgcttcc atgcgccggg cgacggcgaa gaagttgagg tcgtcgagcg ccttgaagcc
     2341 gtcgaagctg accgtcaccc cttcgagata gagcagcgtg tcgccgggac ggggcgccac
     2401 ggcggcaacc tcctcgggcg gctggacgcg gagcgcggtg tcaaccatcg gcggcggcct
     2461 gcgccgggcg cgcgcgcgca ccgcccgcga gcgccaggcg cagcgagcgg acggcgccga
     2521 ccacgccctt gggcaggaac agggtgacgg cgatgaacag cgcgccgagc aggtacagcc
     2581 agacctcggg cagggcgccg gtgaaggtgc ttttcgcgta gttgaccagg accgcgccga
     2641 gggcggcgcc gaacagggtg ccgcggccgc cgaccgcgac ccagatgacg atctcgatcg
     2701 agttggcggg cgagaactcg cccggattga tgatgcccgc ctggggcacg tacagcgcgc
     2761 cggcgatgcc ggcaagcacc gccgagacga cgaacgcaaa cagcttgtag tgctcgaccc
     2821 gatagccgag gaagcgggtc cggctttcgg cgtcgcggac cgcgaccagc acccggccga
     2881 agcgcgaggt gaccagagcc cggcaggcga ggtagccgag cgcgagcgcc gccgccgagg
     2941 cggcgaacag cccggcgcgg gtgccgtcgg cctgcaggtc gaagccgagc aggtccttga
     3001 agtcggtaag cccgttgttg ccgccgaagc ccatgtcgtt gcggaagaag gcgagcatca
     3061 gggcgaaggt cagcgcctgg gtgatgatcg acaggtagac gccggtgacc cgcgagcgga
     3121 aggcgagcca gccgaacagg aacgcaagcc cgcccggcac cagcaccacc atcagggcgg
     3181 cgaaggcgaa gccgtcgaag ccgtgccagt accacggcag ctcggtccag ttcaggaaca
     3241 ccatgaaatc gggcagctcg gggtggccgt agacgccgcg ggtgccgatc tggcgcatca
     3301 ggtgcatgcc catggcgtag ccgccgaggg cgaagaacgc gccgtggccg aggctgagga
     3361 tgccgcaata gccccacacc aggtcgacgc tgagcgcgag cagggcgaag cacaggtact
     3421 tgccgagcaa actgacaatg taggtgggca cgtgcagcgc cgaatccgcg ggcaccgaca
     3481 ggttgaacgc cggcaccgcc accgcggcca gcgcgagcgc cgcgagcagc agccggccgc
     3541 cgaggtcgat ccgcgaccac aggctgcgtg cgccgggcac ggctcaggcc tccgcggcgc
     3601 ggcccctgag cgcgaacatc ccgcgcggcc gcttctggat gaacaggatg atcgccacca
     3661 agacaaagat cttgccgagc acggcgccgg ccagcggctc gaggaacttg ttgaccacgc
     3721 caagcgtcag cgcgccgacc agcgtgcccc acaggttgcc gacgccgccg aacacgacca
     3781 ccatgaacga atcgacgatg tagccgcggc ccagattcgg gctgacgttg tcgatctggc
     3841 tcagcgccac cccggcgatt cccgccaccc cgcagccgag gccgaaggtg agcgcgtcga
     3901 tcatgccggt gcggatgccc atcgatgcgg ccatcgcccg gttctgggtg accgcgcgca
     3961 tgcgcagccc gaacggcgtg cgcgcgatca ccagcaggag cagggcaaag accgcgagcg
     4021 cgaaggcgat gatccagatg cggttgtagg tcagcaccac ggcgccggcg aggtcgatgg
     4081 cgccggtcat ccaggacggg ttgccgacct cgcggttggt cgggccgaag acgctgcgca
     4141 cgaactgctg cagcaccagg ctcaggcccc aggtggcgag cagggtctcg agcgggcggc
     4201 cgtagaggaa acggatcagg ccgcgctcga cggcaacccc gaaggcgccg accagcacga
     4261 acgccgccgg caccgccacc gccagcgacc agccgaaggc gcccggcagg taggcccgga
     4321 acagctcctg gacgacgaag gtggtgtagg cgccgagcat caccatctcg ccatgggcca
     4381 tgttgatcac gcccatgacg ccgaaggtga tggcgaggcc gatggcggcg agcagcagca
     4441 ccgagccgag gctcgcgccc tgaaacagcg agcccagtag ctgccagcgc tcgatgcgcg
     4501 cctcgatgcg cgcgagcgcg gccgcggcgg cggcgcgcac ctcggcgtcg ggctcggcga
     4561 agctgccgtc gtcgcgcgcg accagcagcg aggcaacgag ggcgcgcgag tccgaatcgg
     4621 tgaacccggc aagggtctcg accgccgcga tgcgcgccgc cggctccttg gacaatgcca
     4681 gccggccggc ggcaagcgcc cgctcgaggc gcgcgcgaac cgccgcatcg gcctcggcgg
     4741 cgagcgcgtc ggcgagcagc gcgagccccg agtcgtcggg cttcttgaac accgcgtccg
     4801 cggccgccat gcgcttggcg cggtccgggc tcatcagcgt cagccgcccg atcgccgcgc
     4861 gcaacgtgcc gcgcaggcgg ttgttgacct tgatcttgtc gagcgccttg gcctcggccg
     4921 cgccgagcgc cgcgccgctc agcggatcgg tgagcgcgta gccgccgcca gccttgtcgg
     4981 ccaccaccac ggcaccatcg tccgtgcgca agtacagccg gccgtcgagc atcgcctgca
     5041 atgcgccgcc cgcgcgctcg tcgccggtgg cggcgagcgc ctcggccgcg gcgatcttgc
     5101 cgtcgaagcc gctcgcggcg agcgccgtga ccagcgcctc gatgtcttcg gcgcacgcgg
     5161 cgccggcgcc gagcagcagg gctccgccga ccaggacacg gaccagtcgt cgaattggga
     5221 ccatggtgac gaacggctcc cggttggcgg ccgcccgagg ggcggccggg acggaccgcg
     5281 gcgccgcgtt ccgccccggc cggtcggggc cagggagatc agtagctgat cttgtaaagc
     5341 ggcgcctcgc agttgccgca cacccacggg aaggtccagt cggcggtcag cttggcgctc
     5401 tcggggatga agtcgctcca ggcgtcgccg acgaccacgc cgtcggtctg ccagacgacg
     5461 tcgaactggc catcgtcctg gatctcgccg atcatcaccg gcttggacag gtggtggttg
     5521 gtgttcatga ccacgtcgta gccgctgagc gagcgcaccg tctggccgta catcgcctgg
     5581 cgaacggcat cgacctcggt ggtgccggcc tgggcgaccg cctgggccca catgtggaag
     5641 ccgatgtagt gggcctccat ggggtcgttg accacgcgct tagcgttgcc gatgaacgcg
     5701 tgccacttgt cgatgaacgc cgcgttctcg ggcgaatcca ccgacatgaa gtagttccag
     5761 gcggcgaggt ggccgaccag gggcgcggta tcgaggccgg acagctcctc ctcgccgacc
     5821 gagaacgcga ccaccggcac gtcctcggcg ctgatgccct ggttggcgag ctccttgtag
     5881 aacggcacgt tggcgtcgcc gttgatggtc gagaccaccg ccgtcggctt gccctgggag
     5941 gcgaacgccg ccacctcgga gacaatggtc tgccagtcgg aatggccgaa cggggtgtag
     6001 gtctccagga tgtcgtcgtc gccgacgccc ctggcatgga ggaacgcgcg caggatcttg
     6061 ttggtggtgc gcggatagac gtagtcggtg ccgagcagca cccagcgctg ggcaccgccg
     6121 ccggcctcgc tcatcaggta ctcgaccgcc gggatcgcct gctggttggg cgccgcgccg
     6181 gtgtagaaga cgttgcgcga gctttcctcg ccctcatact ggaccggata gtacaaaagg
     6241 ccgtcgagct cctcgaacac cggcagcacc gacttgcgcg acaccgaggt ccagcagccg
     6301 aacaccgccg ccaccttgtc cttctggagc agctcgcgcg ccttctcggc gaacagcggc
     6361 cagtcggacg ccgggtcgac caccaccgcc tcgacctgct tgcccaacag cccgccttgg
     6421 gcgttgagct cggcgaccag catcaggatg gtgtccttga gcgtcgtctc gctgatcgcc
     6481 atcgtccccg acagcgaatg cagcacgccg atcttgatcg tgtccgcgcc cacggccgcc
     6541 ggcgcggtga agccggcggc ggcgaccgcg gcggccacgc aggcgcgccc gagcatgcgc
     6601 gtgatcctcg tcatgacaac ctccaacgcg agaaattttg caccgcacca cagggagcaa
     6661 ggcctgtgcc agccaccgcg cgccgcgcgg accggcgaat ggaatcaaga gcttggccaa
     6721 tgagtccgga ccccgggcgc gagccgtgtc cgttcgcgat gcggacattc ctgattaatc
     6781 aattatcact ttggaagtcc ctagactaaa aattaggcgg aaatcagacc cattccgcac
     6841 cgcttgacag cactcggcac gcgcatacct tggcccgccg aagcctgttg cgaccttcac
     6901 gcgaggtctt ttgcaccaca acgggcctca ggggccgcgg ggcggcgcag tccccggggc
     6961 cccgcccgag gtcgcgacgg cgacggcacc cgcccgctgg gattaaccca tgctggtgcg
     7021 aacgacggta gcatgcgcga gatttgggcg gccggaatgg ggcggttgtc ccgacggccg
     7081 caggacgcaa cagggcgaag acggaggcgc acatggcacg cacatccaag acgaccacta
     7141 ccaagcccgc cgccaagacc ggtgccaagc cggcggcacg caggaagcgc gcggcgccca
     7201 aggccgaggt gccggcagcg gcgcggaagg ccgaggtgcc ggcggtccgc tcggtgttcg
     7261 accagtggct gcagctcagg aacgaggtcg accgcgtgtt cgagaacttc accgccggtt
     7321 tccgcttccc cggcttcgcc tcgcgcatgt tcgacctgga ggcctggccg ctgccgcgcc
     7381 tgctgtcgca acagctcttg atgcgcaccg acctcagcga gaccgacaag gcggtcgagc
     7441 tgcgcgccga gcttccgggc ctgaccgagg ccgacatcga cgtgaccgtg tccgacgacg
     7501 tgctcacggt caccggcaag aagcaggagg agcgggaaga gaggggcaag gagttccacc
     7561 tgcgcgaacg gtcgttcggc tcgttcaagc gctcgttccg gctgccggcg ggggtcgacg
     7621 ccagcaagat cagcgccgca ttcgacaagg gggtgctgac cgtgaccgtg cccaagccgg
     7681 aacggccgaa ggcgccgcgc aagaagatcg ccgtcgcgcg caaggcctga gcgccgcggc
     7741 ctggaaggaa tggcgacatg ggcaagcaac tcgagccgct ggcctcggtc ctcgacgaca
     7801 tggccgaggc cctgcgcgag atgtccgaga tgatctacga caacttctcc gaggcgacca
     7861 acgcccgcgg cgtgctgcgc gcgctgatcc tcgacggcga ccccgaggag gccggcgccg
     7921 agctggtgcg ctgggccgag cggcgcgccg gccagccgag caaccttgac cgtgccaagg
     7981 acctggccga ccggatcgag cggctcaagg ggcggatcaa cgcgctggct gtcgactgag
     8041 cggctgcgcc cgccggcgcc tgtcgggcgc cggcgatgag cagcgccgac ctggtggcct
     8101 tgctcctgtg cggcgacgtc atgaccgggc gcggcatcga ccagatcttg ccgcggccga
     8161 gcgacccgcg cctgtgcgag agctacgccg catcggcggc cgactatgtc gggctcgccg
     8221 agcgcgccca cgggccgatc ccgaggccgg tggcgcccgc ctatgtctgg ggcgccgcgc
     8281 tcgaggcgct ggcccgcgcc cggcccgacc tcggcatcgt caacctggag accgcggtca
     8341 ccaccagcga cgactgcgtc gccaagggcg tcaactatcg gatgcacccg gcgaatgttg
     8401 cctgccttgc cgccgccggc atcgactgct gcgtgctcgc caacaaccac gtcctcgact
     8461 ggggccgggc cgggctggtc gagaccctgg cgacgctcga gggcgccggg atcaagaccg
     8521 ccggcgccgg acgcgaccgc gctgccgcgg cggcgccggc ggtgctcgag gccgccggca
     8581 aggggcgcgt gctggtgttc gcctacggca tggccggcag cggcgttccg cccgactggg
     8641 cggccgaggc cgaccggccc gggatcaatt acctcgccga gccgtcggtg gacgcggcgc
     8701 atcgcattgc cgaagccgtc agcgcggtgc gccggccggg agatgtggtg gtggtgtcga
     8761 tccactgggg cggcaactgg ggctaccagg ttcccgaggc gcagcgccgg ttcgcgcacc
     8821 gcctgatcga cgccggcgcc gccgacgtga tccacggcca ctcctcgcac cacgtcaagg
     8881 cgatcgaggt ctaccgcgac cggctgatcc tctacggttg cggcgatgtc ctcaacgact
     8941 acgagggcat cggcggctac caggagtacc ggggcgacct cgcgctgatg tacctcgcgc
     9001 gcatcgtcgt cggcaccggc gagctcgccg gcctcgccct gatgccgttc cggatgcgca
     9061 agttctccct cgagcgcccg ccggcggcgg acatcgcctg gctcttggag gtgatcgagc
     9121 gcgagtgccg gcggttcggc acccgcatcg ccgcgcgcga cggctacgac ctggtgctcg
     9181 gctgggactg aggccgtgcc gcgaccgcgc cctcgggcgg gcgctggcgc aacagcaggt
     9241 cgctcacctc aatcccatca cgcacgttga cccgaagctc gatctgtctg atcatcggcc
     9301 tccgccggtt ccatgtccgt ttgctccggc cccgtggccg ctgaaccatc gcatgttgcc
     9361 ccatgactcc atctcgaccc ggaaaccaac aaatcatgct gcttcaagtt gttcacaaac
     9421 ccggcacggt ccctcccaca gccgacgaac ccggccagga attatgggcc ggcggatatc
     9481 aggccgaccg cgccgaagaa accgatgaac ttaccacctc cctcgccatt gcttctgagg
     9541 ggcaactgga aataggcaca tggtaccatc tttccgacct gaaagcatat ttggcacccg
     9601 caaaacaaga cgatacgtgg accgagatca tggtgcctgt agaccgaccc gacaaccgcc
     9661 acaataccca cacgttgacg cagcaagtct ttgcaatcaa tacaatcatt aacgcaatcg
     9721 tagcgctttt cgaaaaatta acaccaaacg aagatccttg gatccgtgtc gaataattcc
     9781 gcacttgtaa ctcttactga cttttccgag cgggctttgc ttcgtgccct caccgcaggg
     9841 caatatcaca tatcttatac tgataacact cacttaaaag acaatatttg gacttcaatc
     9901 ctcgatctca ata
//
LOCUS       DASWHU010000162         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2303104, whole genome shotgun sequence.
ACCESSION   DASWHU010000162 DASWHU010000000
VERSION     DASWHU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303104"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..715)
                     /gene="tenA"
                     /locus_tag="VGB88_06135"
     CDS             complement(38..715)
                     /gene="tenA"
                     /locus_tag="VGB88_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX9647057.1"
                     /translation="MAGGGLFARLKAAAAEDWHAYCHHRFVAGIADGSLPEAAFRHYL
                     VQDYLFLIQFARAYALAAFKADGIADMRSAAAVLQAILDVEMQLHLDHCATWGLDEHD
                     MAAAPEALETTAYTRFVLDCGQRGDALDLAAALAPCVVGYGEIGRRLAADPATVGRGN
                     RYASWIETYAGAAYQEVAGAAVAQLDRLWAARGSDARLKPLARLFAQATRLECAFWDM
                     GWRAGAG"
     gene            complement(719..1051)
                     /locus_tag="VGB88_06140"
     CDS             complement(719..1051)
                     /locus_tag="VGB88_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family 2Fe-2S iron-sulfur cluster
                     binding protein"
                     /protein_id="HEX9647058.1"
                     /translation="MPKVTFVEPGGVRHEIEAPVGLTLLEIAHRNDIDIEGACEGSLS
                     CSTCHVIVDPDDYERLPVASEDEEDMLDLAFGLTPTSRLGCQIVFTEELDGLVVTLPA
                     ATRNLLLG"
     gene            complement(1079..2284)
                     /locus_tag="VGB88_06145"
     CDS             complement(1079..2284)
                     /locus_tag="VGB88_06145"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011477609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX9647059.1"
                     /translation="MTESANIRLPIYLDNQSTTPLDARVLEAMMPYLTEKFGNPHSRS
                     HAYGREAEQAVESARAEVARIIGAEPREVIFTSGATESNNLALKGVAGFYRDRRDHVV
                     TVETEHKCVLETCRRLERDGFAATYLPVGPGGLIDLDRLRAAIGERTVLVSVMGVNNE
                     IGVIQPLAEVGRICAERGAFFHTDCAQAVGKIALDVTEMGIDLMSISGHKLYGPKGIG
                     ALYVRRRPRVRLEPLIDGGGQERGLRSGTLPAPLCVGFGAACRIAAAEMAAEESRLRA
                     LRDRLHRGIVARVPDVALNGDADRRVAGNLNLSFAGVDADALMESMTEVAVSSGSACS
                     SAAIEPSYVLRALGLDAARAQCSIRFGVGRFNTEAEIDYAIEAVADHVARLRAQAPRT
                     RVRAGAAPD"
     gene            complement(2292..>2837)
                     /locus_tag="VGB88_06150"
     CDS             complement(2292..>2837)
                     /locus_tag="VGB88_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9647060.1"
                     /translation="ALVVRDGVALAPLFAGGGQERRRRAGTENLAGIAGFGVAARLAL
                     EDLATTARLAALRDRFEAEVAALAPEAPVYGRGAPRLANTSCIGMPGVAAETQVMALD
                     LDGVAVSAGAACSSGKVTRSHVLAAMGVAPAPAGEAIRVSLGWQSGEDDVRRLTEAWR
                     RLYLRTRARVRGEMPAAVTAA"
BASE COUNT          379 a         1050 c         1042 g          366 t
ORIGIN      
        1 gtcaacatgg cacccggcgc agccgttgca aggccgctca gcccgcgccg gcgcgccagc
       61 ccatgtccca gaacgcgcat tcgaggcgcg tcgcctgggc gaacaggcgc gcgagcggct
      121 tgaggcgggc gtcgctgccg cgcgccgccc acagccggtc gagctgggcg accgccgcgc
      181 cggcgacctc ctggtaggcg gcgccggcgt aggtctcgat ccagctcgcg taacgatttc
      241 cgcgcccaac cgtcgccggg tcggcggcga gccggcggcc gatctcgcca tagccgacca
      301 cgcacggcgc gagcgccgcg gcaaggtcga gcgcatcgcc ccgctggccg cagtcgagca
      361 cgaagcgggt gtaggcggtg gtctcgagcg cctcgggcgc ggccgccatg tcgtgctcgt
      421 caaggcccca ggtcgcgcag tggtcgaggt ggagctgcat ctcgacgtcg aggatcgcct
      481 gcagcaccgc cgccgcgctg cgcatgtcgg cgatgccgtc ggccttgaac gcggccagcg
      541 cataggcgcg cgcgaactgg atcaggaaca gatagtcctg caccagatag tggcggaacg
      601 ctgcctcggg caggctgccg tcggcgatgc cggcgacgaa ccggtgatgg cagtaggcgt
      661 gccaatcctc ggcggcggcg gccttgaggc gcgcgaacag gccgccgccg gccatcgctc
      721 agccgagcaa cagattgcgg gtcgcggccg gcagggtgac caccagcccg tccagctcct
      781 cggtgaacac gatctggcag ccgagccgcg aggtgggcgt gaggccgaag gcgaggtcga
      841 gcatgtcctc ctcgtcctcg ctcgccaccg gcaggcgctc gtagtcgtcc ggatcgacga
      901 tcacgtggca ggtcgagcag gacagcgagc cctcgcacgc gccctcgatg tcgatgtcgt
      961 tgcggtgcgc gatctcgagc agggtcagcc cgaccggcgc ctcgatctcg tggcgcacgc
     1021 cgcccggctc gacgaacgtc accttcggca tcgcgtccgg gcgcccgccc gcagccggtc
     1081 agtccggcgc cgcgccggcg cggacgcgcg tccgcggcgc ctgcgcgcgc aggcgggcga
     1141 cgtgatcggc gaccgcctcg atcgcatagt cgatctcggc ctcggtgttg aaccggccga
     1201 cgccgaagcg gatcgagcac tgcgcgcggg cagcgtcgag gccgagcgcg cggagcacgt
     1261 aggacggctc gatcgccgcc gagctgcacg ccgagcccga cgacaccgcg acctcggtca
     1321 tcgactccat cagcgcgtcc gcgtcgacgc cggcgaagct gaggttgagg ttgccggcga
     1381 cgcgccggtc ggcgtcgccg ttcaaggcca cgtcgggcac gcgcgcgacg atgccacggt
     1441 gcaggcggtc gcggagcgcg cgcaggcgcg actcctccgc cgccatctcc gccgcggcga
     1501 tccggcacgc cgcgccgaag ccgacgcaca gcggcgccgg cagcgtgccc gagcgcaggc
     1561 cgcgctcctg gccgccgccg tcgatcagcg gctcgaggcg gacccgcggg cgccggcgca
     1621 cgtacagcgc gccgatccct ttcggcccat acagcttgtg gcccgagatg ctcatcaggt
     1681 cgatgcccat ctcggtcacg tcgagcgcga tcttgcccac cgcctgggcg cagtcggtgt
     1741 ggaagaacgc gccgcgctcg gcgcagatgc gcccgacctc ggccagcggc tggatgacgc
     1801 cgatctcgtt gttgaccccc atcaccgaca ccagcacggt gcgctcgccg atcgcggcgc
     1861 ggagccggtc gaggtcgatc agcccgcccg ggccgaccgg caggtaggtc gccgcgaagc
     1921 cgtcgcgctc gagccgccgg caggtctcga gcacgcattt gtgctcggtc tcgacggtga
     1981 cgacgtggtc cctgcggtcg cggtagaagc cggcgacgcc cttgagcgcg aggttgttcg
     2041 actcggtggc acccgaggtg aagatcactt ccctgggctc ggcgccgatg atccgggcga
     2101 cctcggcgcg cgcgctctcg accgcctgct cggcctcgcg gccgtaggcg tggctgcgcg
     2161 aatgcgggtt gccgaatttc tcggtcaggt acggcatcat cgcctcgagc acgcgcgcgt
     2221 ccagcggcgt ggtcgactgg ttgtcgagat agatcggcag cctgatgttg gcgctctcgg
     2281 tcatctccgc ctcacgccgc ggtcaccgcg gccggcatct cgcctcgcac gcgggcgcgg
     2341 gtgcgcagat agaggcggcg ccacgcctcg gtcaagcgcc gaacgtcgtc ctcgccgctt
     2401 tgccagccga ggctgacgcg gatcgcctcg ccggcgggcg caggggcgac ccccatcgcc
     2461 gccagcacgt ggctgcgggt gaccttgccc gagctgcacg ccgcgccggc gctgaccgcg
     2521 acgccgtcga ggtcgagcgc catcacctgg gtctcggcgg cgacgccggg catgccgatg
     2581 cagctggtgt tggcgagccg gggcgcgccg cggccgtaga ccggcgcctc gggggcgagc
     2641 gccgcgacct cagcctcgaa gcggtcgcgc aacgccgcga gccgcgcggt cgtcgcgagg
     2701 tcttccagcg ccaggcgcgc ggcgacgccg aagccggcga tgccggccag gttctcggtg
     2761 ccggcgcgcc ggcgccgctc ctggccgccg ccggcaaaga gcggcgcgag cgcgacgccg
     2821 tcgcgcacca ccagcgc
//
LOCUS       DASWHU010000163        11300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_810557, whole genome shotgun sequence.
ACCESSION   DASWHU010000163 DASWHU010000000
VERSION     DASWHU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810557"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB88_06155"
     CDS             <1..379
                     /locus_tag="VGB88_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX9647061.1"
                     /translation="MCRPVVRRWIDIGMLAGAIEPPRGMDPRALYRVAWLPQRWAYIH
                     PVQDVQADRDEVRGGLSSRSQKVAQRGYDPAEIDRENAEDNARADALGLAYDSDGRQP
                     RSGGAAKTADAEEREDEREIENA"
     gene            372..1289
                     /locus_tag="VGB88_06160"
     CDS             372..1289
                     /locus_tag="VGB88_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX9647062.1"
                     /translation="MRDLSLVATRVLNAPLLAHPGKAEVVAAVLLGRNGVEVNVEASA
                     FASAPAPAMGPLQERRLVAWCDAERRPYLFDEQSGIAVIEVIGSLAHRQGSVGAYSGV
                     MGYDWLGAQLDAAIEADDARALVFDLHTPGGEVDGAFQLADRIAAARGVKPMVAVVDE
                     MAFSAGYLLAAACDEIWLASDVSQVGSVGVVWVHFSFEEFLAGAGIKPTIVQAGARKA
                     DGNPFQELPEEVRDLVQAKVDAVYDQFVSRVAAWRGMDEAAVRATEAGIYMGREALGV
                     GFADGIADPSEMFAALAAEVNPTPAVRAV"
     gene            1302..1934
                     /locus_tag="VGB88_06165"
     CDS             1302..1934
                     /locus_tag="VGB88_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647063.1"
                     /translation="MSLSDRLRGRRAAAKAQDDDERDDEESKRAQGEDDDDDKKESKR
                     AQGDDDDDEDSAKSKRSKKAKAQDDDDEDDEGMADDDDKEKAAAPGRVAQLCADAGVP
                     GLARHLIDARLGPRGVRRRIDQAVEIKALGARLRGRWPAVSAAIAEEIAVKGMSVQAA
                     KATFLDLIAANQSGDIRGHHQPGGPAPAGQSADVHGWGNAIAKATGAKNF"
     gene            1974..2363
                     /locus_tag="VGB88_06170"
     CDS             1974..2363
                     /locus_tag="VGB88_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEX9647064.1"
                     /translation="MATKTEGKHAAEFLVSEANGTLSRETATVKSGQNLVAGEVVEKD
                     VDGKLIAFSAGVDSDGAVLVEAVGIMWDNVDASATGANADVPGQVYIARLAEVNDGEI
                     TYPTETSDGGEKALAIASLEKLHIRPR"
     gene            2496..3509
                     /locus_tag="VGB88_06175"
     CDS             2496..3509
                     /locus_tag="VGB88_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEX9647065.1"
                     /translation="MAHMDIFADDAFSMVELTAALDKVPYKPQFLGDLGIFEPRPVRT
                     IHISLEKRDNVLALIQTSERGAPLDERPNEKRDIRNFNTVRIAKADALYAHEIQSIRA
                     FGAESELQQVQQELARRMERIRQDVELTHENMRLGAVQGIVTDADGSTIRNWYTDWDI
                     SQAAEIDFELDDTSTNVRGKCQEVRRAMERNAKGAMPPGTQVHALCDDVFFDAFIDHA
                     RVRDTYLNWAAAEQLRENRAFESFPFAGIIFHNYRGTDDNSTVAVGTDKAKFFPVGAP
                     GVFQVAWSPGESFDVTNTPGLPVYARMIPDRDRNAWIKVEEYSYPLFICTRPEMLQRA
                     KRA"
     gene            3532..4236
                     /locus_tag="VGB88_06180"
     CDS             3532..4236
                     /locus_tag="VGB88_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647066.1"
                     /translation="MTWRIPAEWRGETAFVLGGGESLTGFDASILRGRGRVVAINDAG
                     LILAPWADLLFWSDPRWIEWNHAELHLHTGRWKACRHSAGARTPKCPPALSMRIARAL
                     AEHKVKHLAHDRLGALSRDPGRVAGACAGAGGVNAAFLAGAARIVLLGFDMRPGHWHG
                     RHRKASRVERYPQFAAALARMARSLAAARVETLNARPAPPASAGAGLPAGTESALDCF
                     PKVRLEDVLERVRAAA"
     gene            4233..5054
                     /locus_tag="VGB88_06185"
     CDS             4233..5054
                     /locus_tag="VGB88_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX9647067.1"
                     /translation="MSGIRVFVGCAPDGQDAESQAVLEYTLRLHSSRRVEITWMRLSR
                     NPASPFYSDPALGTGWRTELWATPFSGFRWAVPALCDFEGRAIYMDSDVIVRADIAEL
                     WDQRFEPGKAVMAKRSGRFCVSLWHCGRAARWLRHIDVLGSDPGSHRAMTRLFAKHAN
                     LVQRFAGDWNCLDGERHPDLSDPAVKAIHYTSMPHQPHLARARQRLAARGRKHWFDGT
                     AAPHWRPDLTALFDRLLGEAIAAGFRPEGYEGEAFGAVAKKSLAAMSGTMPSWAR"
     gene            5051..5710
                     /locus_tag="VGB88_06190"
     CDS             5051..5710
                     /locus_tag="VGB88_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647068.1"
                     /translation="MTTVACVWTGGKYSIDYVARLRAMVARHLVVDHRFICLTDHGAV
                     PDGVERIEVPAIGLERWWAKMALFAPAVRGPGPCLYLDLDTVVVGDLGPLAAWAGTPG
                     SSAGAGFAICRNFHAKPGWCRYGSCAMTFADGWGEPVWRRFVADHQGLMARSRWGDQG
                     AIELLVPDAVILQDRVPDGYFVHYKAIPPARPDEAAVVVFGGKARPHDCTVPWVREAW
                     G"
     gene            5710..6561
                     /locus_tag="VGB88_06195"
     CDS             5710..6561
                     /locus_tag="VGB88_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9647069.1"
                     /translation="MAAAGAACVVRPGDRVDPESTWLHNHKRNVASQFGEDGIIQEVV
                     AILGLDRGSDRWCIEIGAWDGYRFSNTWNLIVNGGWHGVLIEGDTKRFAELEKRYAGM
                     ARVTTVRAWVDVKGVHSLDRILLAATKLPGDIDLLSIDIDGADWHVWKRLEAYRPKIV
                     VIEFNPSIPNDVHFVQAADCTVHQGSSLAALIALGKEKGYELVVTTTVNAVFVVADMF
                     ERFGIRDNRIDAMHTNGELESRLFQLYDGTLVLAGCKRLIWSKIDIDPERLQVLQPAE
                     RTFKGCP"
     gene            6552..7097
                     /locus_tag="VGB88_06200"
     CDS             6552..7097
                     /locus_tag="VGB88_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9647070.1"
                     /translation="MPVAPTKLHLGCGRRHIPGWHHVDLMAYRHVDRVGPAERLDHIA
                     DDSVEIIYASHVLEHFGRYEVFDVVSEWYRVLRTGGILRVSVPDLSAICRRYLACGDI
                     REVLGLLVGGQTGPLDCHKTVFDAASLGELMEDVGFAEVRHYDWRETEHHFIDDFSQA
                     YLPHMDKRKGLQMSLNVEAVK"
     gene            7094..7714
                     /locus_tag="VGB88_06205"
     CDS             7094..7714
                     /locus_tag="VGB88_06205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9647071.1"
                     /translation="MTAQPSLANRQAAAAGSIANKAQRADEMTAFLELIAAEGVRSYL
                     EIGVQRGLTLLSVGLTLAPGARLVGVDLPGAAWGVKDGTGPAQIEAACAQLRACGQSV
                     EMIWGNSRAPEVVAAATERGPYDLVFIDGDHSLEGVTADCENYGPMARMFAFHDIDTP
                     NKRGIAPDRLARYGVHMLWPRLKAVYRHREIVGAQRGMGIGVVWRV"
     gene            7702..8406
                     /locus_tag="VGB88_06210"
     CDS             7702..8406
                     /locus_tag="VGB88_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647072.1"
                     /translation="MAGIVADVRGGLGGQVACLLGQCAQAWDAGDELSAVHLNVGEPV
                     APFGRVSWLDWLFERVPRVETVKTKHKLDLEDVSIWAAVARWRGAFAERLGLVDAGPR
                     AGDMQVIHVRSGDRQPASIEALASLAAAAASAVVIGNELSAVQRVADISGHGAVNGDP
                     RSMPGAGAVDDWLLARRAGRVLGAPSYFTLTAAILEPGLEVHWLRDGGGPAPMRAETI
                     RSLEAAMAALGNVRWL"
     gene            8397..9230
                     /locus_tag="VGB88_06215"
     CDS             8397..9230
                     /locus_tag="VGB88_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647073.1"
                     /translation="MALDAMWTGAKDFGRWPELLAVHVERVQRAAAAAQLYLASVDLR
                     SRGLIAVGGLLGPDLAARVAAEVRAVRPGDAKRKDSLVANRAPESPARRAAAAMWTRI
                     GPLWRGHLIEADRLFLRNTYLQHLVNAPGDGDIQKVLHCDTFFPALKFWYFPESVGPD
                     DGPLVYVPGSPVLTAALLDWHRRQAESIAAGTVEPWRGAGHGEGSLRIGDGELAELGL
                     EAEPVTVDADTLVIANVFGFHRRGDAPTRCDRVSVHGSIRISRPLDPGLPRVEPEGRL
                     S"
     gene            9227..10123
                     /locus_tag="VGB88_06220"
     CDS             9227..10123
                     /locus_tag="VGB88_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647074.1"
                     /translation="MKVICFEPGYDEKHRTVLRALAAGIPGAEVCPLGAYEPCDVAVI
                     FGLVKRAYKATWAKQPVLDRHRGDRLLVVESAFVRRGDYWAMGWGGINGRADFRSASV
                     GDDRWRAMGVKTRPWRSPRDAKASRLGPVVVCGQLPRDTNVQDTDHPAWCRHVMAWLA
                     AERIPAVFRPHPRIDDPEVYGIAPALWDTGKIAETLAWARAAVVFNSTSGVDAALAGV
                     PVVALDRGAFCWPIASHRLDVLRDPGSSPGAGPGSSPGAGPRCPSRRAWLAGLGYSQW
                     TLEEMRAGLPWRHLTRALGDDG"
     gene            10113..10838
                     /locus_tag="VGB88_06225"
     CDS             10113..10838
                     /locus_tag="VGB88_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647075.1"
                     /translation="MTVDVAPAAIGWGEVVGTARRVAVLACGPSVRGLDLAALARATS
                     DGIQVILVNRAIGWLPMGCAAGVSWFTLDPDPAMRAIMAHPAPGVAYYAAVPDDYGQP
                     NARWLRHRAPAEPAVSYLRRLTGDGPWQCHYGLSDDPRAIHTGNSAWGGLGVAWHKVR
                     AAGDARIALFGVDGTDEPYAYGQGRPRRPLDHLPDLFASALPQLYARGVEVINGSAAS
                     LITCFPRSSTGEALAWLTAGAGP"
     gene            10854..11150
                     /locus_tag="VGB88_06230"
     CDS             10854..11150
                     /locus_tag="VGB88_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647076.1"
                     /translation="MVDFSVLNTAVEQTFAEVVHWIDAFDRATSGIFDSRHVEIETEG
                     QGVVSELVTTLGVKADEAEPIKTGERCEVRGVLYRIVDRRPDGQGMIVLLLERA"
BASE COUNT         1684 a         3803 c         4192 g         1621 t
ORIGIN      
        1 gatgtgccgc ccggtggtgc gccgctggat cgacatcggg atgctggccg gggcgatcga
       61 gccgccgcgc ggcatggacc cgcgggcgct gtaccgggtc gcgtggctgc cgcagaggtg
      121 ggcgtacatc caccccgtcc aggacgtcca ggcggaccgc gacgaggtgc gcggcggtct
      181 cagctcccgc tcccagaagg tggcgcagcg cggctacgat ccggccgaga tcgaccgcga
      241 gaacgccgag gacaacgcgc gcgccgacgc actcgggctc gcctacgaca gcgacggccg
      301 gcaaccgcgc agcggcggcg cggccaagac cgccgacgcc gaggagcgcg aggacgagcg
      361 ggagatcgag aatgcgtgat ctctcgctgg tcgcgacgcg ggtgctgaac gcgccgctgc
      421 tcgcgcatcc gggcaaggcc gaggtggtcg ccgcggtgct gctgggccgg aacggcgtcg
      481 aggtcaacgt cgaggcctcg gcgttcgcca gcgcgccggc cccggcgatg gggccgctgc
      541 aggagcggcg attggttgcg tggtgcgatg ccgagcgccg gccgtacctt ttcgacgagc
      601 agagcggcat tgcggtgatc gaggtgatcg gctcgctggc gcaccgccag ggctcggtgg
      661 gcgcttactc cggcgtgatg ggctacgatt ggctcggcgc acagctcgac gccgccatcg
      721 aggccgacga cgctcgagca ctggtcttcg acctgcacac gccgggcggc gaggtcgacg
      781 gcgcgttcca gctcgccgac cggatcgcgg cggcgcgcgg cgtcaagccg atggtggcgg
      841 tggtcgacga gatggcgttc agcgccggct acctgctggc cgcggcgtgc gacgagatat
      901 ggctcgcctc cgatgtgagc caggtcggct ctgtcggcgt ggtgtgggtg catttcagct
      961 tcgaggagtt cctggccggc gccggaatca agccgacgat cgtccaggcc ggcgcgcgca
     1021 aggctgacgg aaacccgttc caggagctgc ccgaggaggt ccgcgacctg gtgcaggcca
     1081 aggtggacgc tgtctacgac cagttcgtga gccgggtcgc ggcgtggcgc ggcatggacg
     1141 aggccgcggt gcgcgccacc gaggccggta tctacatggg ccgcgaggcg ctgggcgtcg
     1201 gcttcgccga cggcatcgcc gacccgtccg agatgttcgc ggcgctcgcc gccgaggtca
     1261 accccacgcc cgcggtgcgg gccgtttgag aggaggctgt gatgagcctg tccgaccgac
     1321 tgcgcgggcg ccgagctgcg gcgaaggccc aggacgacga cgagcgcgac gacgaggagt
     1381 ccaagcgcgc ccagggcgag gacgatgacg acgacaagaa ggagtcaaag cgcgcccagg
     1441 gcgacgacga cgatgacgag gactcggcca agtcgaagag gtccaagaag gccaaggccc
     1501 aggacgacga cgacgaggac gacgagggca tggccgacga cgacgacaag gagaaggccg
     1561 ccgcgcccgg ccgggtcgcg cagctgtgcg ccgacgccgg ggtgccgggc ctggcccgcc
     1621 acctgatcga cgcccggctg ggcccgcgcg gggtgcgccg gcgcatcgac caggcggtcg
     1681 agatcaaggc tctgggcgcg cgcctgcgcg gccggtggcc ggcggtcagc gcggcgatcg
     1741 ccgaggagat cgcggtcaag ggcatgtcgg tccaggcggc gaaggcgacg ttcctcgacc
     1801 tgatcgcggc caaccagtcg ggcgacatcc gcggccacca tcagcccggc ggacccgctc
     1861 ccgcgggcca gagcgcagac gtgcacggct ggggcaacgc gatcgccaag gcgaccggcg
     1921 ccaagaactt ctgagcgccg agcgccccgt gattcaagag taggaggcca gcgatggcta
     1981 ccaagacgga aggcaagcac gccgccgagt tcctggtcag cgaggccaac ggcacgctga
     2041 gccgcgagac cgcgacggtg aagagcggcc agaacctcgt cgccggcgag gtggtcgaga
     2101 aggacgtcga cggcaagctg atcgcgttca gcgccggcgt cgacagcgac ggcgcggtcc
     2161 tggtcgaggc cgttggcatc atgtgggaca acgtcgacgc ctcggcgact ggcgccaacg
     2221 ccgacgttcc gggccaagtc tacatcgccc ggctcgccga ggtgaacgac ggcgagatca
     2281 cctacccgac cgagaccagc gacggcggcg agaaggctct cgccatcgcc agcctcgaga
     2341 agctccacat ccggccgcgc taagcggcgg cccccccgcc ttcgcggggg caagcttgcc
     2401 ggcataggcc ggcgcccgtt ccatcatcgc gctgatccgg ccgccggcct ttagggccgg
     2461 gacggcgcgg ctcggcccgt cgataggagg ccctcatggc ccacatggac atcttcgccg
     2521 acgacgcctt ctcgatggtc gagctgaccg cggccctcga caaggtgcct tacaagccgc
     2581 agttcctggg cgatctcggc atcttcgagc cgcgcccggt gcgcaccatc catatctcgc
     2641 tcgagaagcg cgacaacgtg ctggcgctga tccagaccag cgagcgcggc gcgccgctcg
     2701 acgagcggcc gaacgagaag cgcgacatcc gcaacttcaa caccgtccgg atcgccaaag
     2761 ccgacgccct gtacgcgcac gagatccagt cgatccgcgc gttcggcgcc gagtccgagc
     2821 tgcagcaggt gcagcaggag ttggcgcggc gcatggagcg catccgccag gacgtcgagc
     2881 tgacccacga gaacatgcgg ctcggcgccg tgcagggcat tgtcaccgac gccgacggct
     2941 cgacgattcg caactggtac acggattggg acatcagtca ggcggcggag atcgacttcg
     3001 agctcgacga cacctcgacc aacgtgcgcg gcaagtgcca ggaggtgcgc cgcgcgatgg
     3061 agcgcaacgc gaaaggcgcc atgccgccgg ggacgcaggt gcatgcgctg tgcgacgacg
     3121 tgttcttcga cgccttcatc gaccacgcgc gggtccgcga cacctatctg aactgggccg
     3181 ccgccgagca gctgcgcgag aatcgcgcgt tcgaaagctt cccgttcgcc ggcatcatct
     3241 tccacaacta ccgcggcacg gacgacaact cgacggtcgc ggtgggcacc gacaaggcga
     3301 agttcttccc ggtcggcgcg cccggagtgt ttcaggtggc gtggagcccg ggcgagagct
     3361 tcgatgtcac gaacactcca ggcctgccgg tctatgcacg catgatcccc gaccgcgacc
     3421 gcaacgcctg gatcaaggtc gaggagtaca gctacccgct gttcatctgc acccgcccgg
     3481 agatgctgca gcgcgccaag cgcgcctgat tcccgcctgc gcggggacgg ggtgacctgg
     3541 cgcatccccg ccgagtggcg gggcgagacc gcgttcgtcc tcggcggcgg cgagtcgctg
     3601 accgggttcg acgcctcgat cctgcgcggg cgcgggcgcg tggtcgcgat caacgacgcc
     3661 ggcctgatat tggcgccgtg ggccgacctg ctgttctggt cggatccgcg ctggatcgag
     3721 tggaaccatg ccgagcttca cctccacacg gggcggtgga aggcgtgccg gcacagcgcg
     3781 ggggcgcgca cgcccaaatg cccgcccgcg ctgtcgatgc gcatcgcccg cgcgctggcc
     3841 gagcacaagg tgaagcacct ggcgcacgac cggctgggcg cgctcagccg cgacccgggc
     3901 cgcgtggctg gggcgtgcgc cggcgcgggc ggggtcaacg ccgcgttcct ggccggcgcg
     3961 gcgcggatcg tgctgctcgg cttcgacatg cggcccggcc actggcacgg ccgccaccgg
     4021 aaagcgagcc gggtcgagcg ctacccgcag ttcgccgccg cgctggcgcg catggcgaga
     4081 tcgctggccg cggcgcgcgt cgagacgctg aacgccaggc cggcgccccc cgcttccgcg
     4141 ggggcaggct tgccggccgg caccgagtcc gcgctcgact gctttccgaa agtccggctc
     4201 gaggacgtgc tggagcgcgt gagggcggcg gcgtgagcgg gatcagggtg ttcgtcggct
     4261 gcgcgcccga cggccaggac gcggaaagcc aggcggttct cgaatacacg ctccggctcc
     4321 actccagccg gcgtgtcgag atcacatgga tgcgactctc ccgaaatccg gcgagcccgt
     4381 tctactcgga cccggcgctg gggacgggct ggcggaccga gctgtgggca acaccgttct
     4441 cgggcttccg ctgggccgtg ccggcgcttt gcgacttcga gggccgggcg atctacatgg
     4501 actcggacgt catcgtccgc gccgacatcg ccgagctgtg ggaccagagg ttcgagcccg
     4561 gcaaggcggt gatggcgaag cggtcgggcc ggttctgcgt ctcgctctgg cactgcggtc
     4621 gcgccgcgcg ctggctgcgc cacatcgacg tgctgggcag cgacccgggc tcgcaccgcg
     4681 cgatgacgcg gctgttcgcg aagcacgcca acctggtgca acggttcgcc ggggattgga
     4741 actgcctcga cggcgagcgc catcccgacc tgtcggaccc ggcggtcaag gcgatccact
     4801 acacctcgat gccgcaccag ccccatctcg cccgggcgcg gcagcggctc gcggcgcgcg
     4861 ggcgcaagca ctggttcgac ggcacggccg cgccgcactg gcgcccggac ctgaccgcgc
     4921 tgttcgaccg gctgctcggc gaggcgattg cggccgggtt ccgccccgaa ggctatgagg
     4981 gcgaggcgtt cggcgcggtc gccaagaagt cgcttgccgc gatgagcggc acgatgccga
     5041 gctgggcgcg gtgaccaccg tcgcgtgcgt ctggaccggc ggcaaatact cgatcgacta
     5101 cgtggccagg ctgcgcgcga tggtggcgcg gcatcttgtt gtcgaccaca ggttcatttg
     5161 cctgaccgac cacggggcgg tgccggacgg cgtcgagcgg atcgaggtcc ccgcgatcgg
     5221 cctggagcgg tggtgggcga agatggcgct gttcgcgccg gcggtgcgcg gcccggggcc
     5281 ctgcctgtat ctcgacctcg acacggtggt ggtcggcgat ctcgggccgc tcgccgcgtg
     5341 ggccggcacc cccggctcgt cggccggggc aggcttcgct atctgccgga acttccacgc
     5401 caagcccggc tggtgccggt acggcagctg cgcgatgacg ttcgccgacg gctggggcga
     5461 gccggtctgg cgccgcttcg tcgccgacca ccaaggcctg atggcgcgct cgcgctgggg
     5521 cgaccagggc gcgatcgagc tgctggtgcc cgacgccgtg atccttcagg accgcgtgcc
     5581 ggacggctac ttcgtccatt acaaggcgat cccgccggcg cgcccagatg aggcggcggt
     5641 ggtggtgttc ggcggcaagg cgcggcccca cgattgcacc gtgccgtggg tgcgcgaggc
     5701 gtggggctga tggccgcggc cggcgcggcg tgcgtggtgc gcccgggcga tcgtgtcgac
     5761 ccggaaagca catggctcca caatcacaag cggaacgtcg cctcccagtt cggcgaggac
     5821 gggatcattc aagaggtggt tgccatcctc ggcctcgatc gcggatcgga ccggtggtgc
     5881 atcgagatcg gggcctggga cggctacagg ttcagcaaca catggaacct gatcgtcaac
     5941 ggcgggtggc atggcgtcct gatcgaggga gacacaaagc ggttcgcgga gcttgagaaa
     6001 agatacgccg gcatggcgcg ggtgaccacg gtccgcgcct gggtggacgt gaaaggcgtg
     6061 cactcactgg atcgcatctt gttggctgcc accaagctgc ccggggatat cgacctgttg
     6121 agcatagata tcgacggcgc ggattggcac gtctggaaaa ggctcgaggc ataccgcccg
     6181 aagatcgtgg tgatcgagtt caacccgtcg atccccaacg acgttcactt cgtgcaggct
     6241 gcggattgca cggttcatca gggatcgtcg ctcgcggcgc tgatcgcgct cggcaaggaa
     6301 aagggctacg agctggtcgt gaccacgacc gtcaacgccg tgttcgtcgt tgcggacatg
     6361 ttcgagcggt tcggcattcg agacaaccgc atcgacgcca tgcacacgaa cggcgagctc
     6421 gaaagcaggc tgtttcagct ttacgacggg acgctggtgc tggccggatg caagaggctg
     6481 atctggtcga agatcgacat cgacccggag cggctacagg tcttgcagcc ggcggagcga
     6541 acgttcaagg gatgcccgta gctccgacga agctgcacct gggctgcggc cggcggcaca
     6601 ttcccggatg gcatcacgtc gacctgatgg catatcggca cgtcgacagg gtcgggccag
     6661 cggaaaggct ggaccatatc gccgacgact cggtggagat catttacgcc tcgcacgtgc
     6721 tcgagcattt cggccgctac gaggtcttcg acgtggtcag cgaatggtac agagtgctga
     6781 ggaccggcgg gattctgcgg gtctcggttc ccgacctctc agcgatctgc aggaggtatc
     6841 tggcctgcgg cgacatccgc gaggtgctgg gcctgctggt cggcggacag accgggcctc
     6901 tcgactgtca caagacggtg ttcgacgcgg ccagcctcgg cgagctgatg gaagacgtcg
     6961 ggttcgcgga agtccggcac tacgactggc gagaaaccga gcaccacttc atcgacgatt
     7021 tcagccaggc ctacctgccg cacatggaca aacggaaggg gctgcaaatg agcctgaacg
     7081 tggaagcggt caagtgacgg cgcagccgag cctcgcgaac cgccaggcgg ctgccgccgg
     7141 ctcgatcgcg aacaaggcgc agcgcgccga cgagatgacc gcgttcctcg agctgatcgc
     7201 ggccgagggc gtgcgcagct acctcgagat cggcgtccag cgcgggctga ccttgctgtc
     7261 ggtcgggctg accctggcgc cgggcgcgcg gctggtcggc gtcgacctgc ccggcgcggc
     7321 gtggggggtg aaggacggca ccggcccggc gcagatcgag gcggcgtgcg cacagctgcg
     7381 cgcgtgcggc caatcggtcg agatgatctg gggcaatagc cgggcgcccg aggtggtcgc
     7441 cgccgccacc gaacgcggcc cctatgacct ggtgttcatc gacggcgatc actcgctcga
     7501 gggtgtcacc gccgattgcg agaattacgg accgatggcg cggatgttcg cgttccacga
     7561 catcgacacg ccgaacaagc gcggcatcgc gcccgaccgc ctggcgcgct acggcgtgca
     7621 catgctctgg ccgcgcctca aggcggtcta tcggcaccgc gagatcgtcg gcgctcagcg
     7681 cggcatgggg atcggcgtgg tgtggcgggt atagtcgccg acgtccgcgg cgggctcggc
     7741 ggccaggtcg cgtgcctgct cggccagtgc gcgcaggcct gggacgccgg cgacgagctg
     7801 agcgcggtgc atctgaatgt gggcgagccg gtggcgccgt tcggccgggt gagctggctc
     7861 gactggctgt tcgagcgcgt cccgcgagtc gagaccgtga agaccaagca caagctcgac
     7921 ctcgaggacg tgtcgatctg ggcggcggtg gcgcgatggc gcggcgcgtt cgcggagcgg
     7981 ctcggcctgg tcgacgccgg gccgcgtgcc ggcgacatgc aggtaatcca tgtgcgcagc
     8041 ggcgaccgtc agccggcgag catcgaggcg ctggccagcc tcgccgcggc cgcggcgagc
     8101 gcggtggtga tcggcaacga gttgtccgcc gtccagcgcg ttgcggacat cagcggccac
     8161 ggcgcggtaa acggcgaccc ccggtcgatg ccgggggcag gcgcggtcga tgactggctg
     8221 ctggcgcgac gtgccgggcg ggtgctgggc gcaccgtcct atttcacgct caccgccgcg
     8281 atcctggaac ccggactcga ggtccattgg ctgcgggacg gcggcggacc ggcgccgatg
     8341 cgcgccgaga cgatcaggag cctggaggcg gcgatggccg ccctcggcaa cgtgcgatgg
     8401 ctctagacgc gatgtggacc ggcgccaagg acttcggccg ctggcccgag ctcctggccg
     8461 tgcacgtcga gcgggtccag cgcgccgccg ccgcggcgca gctctatctc gccagcgtcg
     8521 atctgcgcag ccgcgggctg atcgcggtcg gcgggctgct cggcccggac cttgccgccc
     8581 gcgtcgcggc cgaggtgcgc gcggtgcgac cgggagatgc caagaggaag gactcgctgg
     8641 tcgcgaaccg tgcgccggag agcccggcgc gccgggcggc ggcggcgatg tggacacgga
     8701 tcgggccgct gtggcgcggc catttgatcg aggccgaccg gctgttcctg cgcaacacct
     8761 acctgcagca cctggtgaac gcgccgggcg acggcgacat tcagaaggtg ctgcactgcg
     8821 acacgttctt tccggcgctc aagttctggt acttcccgga gagtgtgggc cccgacgacg
     8881 ggccgcttgt ctacgtgccg ggatcgccgg tgctgacggc cgcgctgctc gactggcatc
     8941 gccgccaggc cgagagcatc gccgccggca cggtcgagcc ctggcgcggc gcgggccacg
     9001 gcgaaggctc gttgcggatc ggcgatggcg agctcgccga gctcggcctc gaggccgagc
     9061 cggtgacggt ggacgccgac accctggtga tcgccaacgt gttcgggttc caccgccgcg
     9121 gcgacgcgcc gacgcggtgc gaccgggtgt cggtgcacgg ctcgatccgc atctcgcgtc
     9181 cgctcgatcc cgggctcccc cgggtcgagc ccgagggcag gctctcatga aggtcatctg
     9241 cttcgagccg ggctacgacg agaagcaccg gactgtgctg cgcgcccttg ccgccggcat
     9301 cccgggcgcc gaggtgtgcc cgctcggggc ctacgaaccg tgcgacgtcg cggtgatctt
     9361 cggcctggtc aagcgcgcct ataaggcgac ctgggccaag caaccggtgc tcgaccggca
     9421 ccggggcgac cgactgctgg tggtcgagag cgcgttcgtg cgccgcggcg attactgggc
     9481 aatgggctgg ggcggaatca acggccgcgc cgatttccgc agcgccagcg ttggcgacga
     9541 ccgctggcgg gcgatgggcg tcaagacccg gccctggcgg tcgccccgcg acgccaaggc
     9601 gagccggctc ggcccggtgg tggtgtgtgg ccagctgccg cgcgacacca atgtccagga
     9661 caccgatcat ccggcgtggt gccggcacgt catggcgtgg ctcgccgccg agcgcatccc
     9721 ggccgtgttc cgcccccatc cgcgcatcga cgatcccgag gtctacggca tcgcgccggc
     9781 gctgtgggac accggcaaga tcgccgagac cctggcctgg gcgcgcgccg ctgtggtgtt
     9841 caactcgacc tcgggagtcg acgcggcgct cgccggggtg ccggtggtgg cgctcgaccg
     9901 cggcgcgttt tgctggccga tcgcctcgca ccgcctcgat gtgctgcgcg accccgggtc
     9961 ttcgcccggg gcaggccccg ggtcttcgcc cggggcaggt ccccgctgcc cgtcgcggcg
    10021 cgcctggctg gccggcctcg gctacagcca gtggacgctc gaggagatgc gggccgggct
    10081 gccgtggcgg cacctgaccc gggcgctggg cgatgacggt tgacgttgcc ccggccgcga
    10141 tcggctgggg cgaggtggtg gggacggcgc gtcgcgtggc ggtgctggcc tgtggaccct
    10201 cggtgcgcgg gctcgacctc gcggcgctcg cgcgcgcgac ctcggacggc atccaggtga
    10261 tcttggtcaa ccgcgcgatc ggctggctgc cgatgggatg cgccgccggg gtgagctggt
    10321 tcacgctcga tccggacccg gcgatgcgcg cgatcatggc gcatcctgcg ccaggggtcg
    10381 cctattacgc tgccgtgccc gacgattacg gccagcccaa cgcccgttgg ctgcgccacc
    10441 gcgcgccggc tgagcctgcc gtcagctatt tgcgccgcct gaccggcgat ggcccatggc
    10501 agtgccacta tggattgtcg gacgatcccc gtgccatcca caccggcaat tcggcctggg
    10561 gcgggctcgg cgtggcctgg cacaaggtgc gggccgccgg cgacgcacgg atcgcgctgt
    10621 tcggcgtcga tggaaccgac gagccctacg cctacgggca ggggcggccg cgccgcccgc
    10681 ttgaccacct gccggacctg ttcgcgtccg cgctgccgca gctctatgcg cgcggcgtcg
    10741 aggtgatcaa tggctcggcc gcctcgctca tcacctgctt cccgcgctcc tcgaccgggg
    10801 aagcgctcgc ctggctgacg gcgggagccg gaccgtaggg agggcgtagg gcgatggtcg
    10861 atttcagcgt tctcaacact gcggtcgagc agaccttcgc cgaggttgtg cactggatcg
    10921 atgcgttcga ccgcgccacc agcggcatct tcgactcgcg ccacgtcgag atcgagaccg
    10981 aggggcaggg cgtggtctcg gagctggtga ccacgctcgg cgtcaaggcc gacgaggccg
    11041 agccgatcaa gaccggcgag cgctgcgagg tgcgcggggt gctgtaccgg atcgtcgatc
    11101 gccggcccga cggccaaggc atgatcgtgc tgctgctcga gcgggcctga tcctgcttcg
    11161 cgctccgcgc ttcgcaggac agaccctcta ttcacggagt ttaggcggac ctgtccgccg
    11221 aagctccgca ggagcgtagg cggatgcaca agcgccaggc gatccgagac gaggcctttg
    11281 tccggctcgg cgccttggcc
//
LOCUS       DASWHU010000164         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2303980, whole genome shotgun sequence.
ACCESSION   DASWHU010000164 DASWHU010000000
VERSION     DASWHU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303980"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..392
                     /locus_tag="VGB88_06235"
     CDS             132..392
                     /locus_tag="VGB88_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647077.1"
                     /translation="MATPRTLDSARAGRRALVLAALLAAAGGCTPRDVTSVENGVWQM
                     VTGHDKPIILEPPPPPVYCYRTIGGPQCEDQPIERDRSAAIR"
     gene            503..2296
                     /gene="aspS"
                     /locus_tag="VGB88_06240"
     CDS             503..2296
                     /gene="aspS"
                     /locus_tag="VGB88_06240"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9647078.1"
                     /translation="MHEYRTHNCGELTLDHVGREVRLSGWVHRKRDHGNLLFIDVRDR
                     FGLTQCVVEQGGAHFEAVAAVRPESVVTVTGKAVARSEDTVNPRLATGHVEVRIADFA
                     VQSAAEVLPMQVAGEQDYSEEMRLRYRFLDLRREHMQRNIRLRADIIASIRRRMIAQG
                     FTEFQTPILTSSSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLIMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDMEMSFATQEDVFAAIEPVLHGVFEEFASWRVSPRPFPR
                     IPFAEAMLRYGTDKPDLRNPILISDVTEAFRMEGVEFKAFRTAIDRGAVVRAIPAPGA
                     AERPRSFFDKLNDWARAEGAPGLGYIVFDAEGAGKGPIAKFVPAAAQAAILEAAGAGA
                     GDAVFFVCMTPSEAAEFAGRVRTRLGDELDLLETDVFRFCWTVDFPLYEPDEETGGVK
                     FSHNPFSMPQGGLEALETSDPLDIRAFQYDIVCNGVELSSGAIRNHRPDIMIKAFAIA
                     GYAAEDVEARFGGMLRAFRFGAPPHGGIAPGIDRIVMLLAHEPNIREVILFPMNQQAE
                     DLLMGAPSEVDDKHLRELHIRVALPPKAKQE"
     gene            complement(2307..2948)
                     /locus_tag="VGB88_06245"
     CDS             complement(2307..2948)
                     /locus_tag="VGB88_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183789.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEX9647079.1"
                     /translation="MPVYDVLSGELVALASVILVDLVLAGDNAIVVGAVAARLPAELR
                     PRAILFGVLAAMVLRIAFAVVASSLLAIIGLVLAGGLLLLWVAWKMWRELEASRVPAP
                     AGAAAGAADPGAAAPARPVSSFRSAIWQIVVADVSMSLDNVLAVAGTARHHVWVLVFG
                     LALSVALMGFAANLVARLIERWRWLGYLGLAVVAVVAVSMIVEGTKQVLAYAA"
BASE COUNT          466 a         1103 c          988 g          399 t
ORIGIN      
        1 ccaccctccg ggcccggtca ccgaaccggg ccgcgagagg agccgcgacc gtcacccttt
       61 ccccttggaa tcgtcagact ggggccgaac tattagcaat tcgttaacga acgccgcgga
      121 cagtggccgc catggccacc ccgaggacct tggacagtgc gcgcgccggg cgccgggcgc
      181 tggtgctcgc ggcgctgctg gcggccgccg gcggctgcac gccgcgcgac gtcacctcgg
      241 tcgagaacgg cgtctggcag atggtcaccg gccacgacaa gccgatcatc ctcgagccgc
      301 cgccgccgcc ggtctactgc taccgcacca tcggcggccc gcagtgcgag gaccagccga
      361 tcgagcgcga ccgcagcgcg gcgattcgtt agcgccgccg actcggccct ttcctcgccg
      421 acccggcccc actatgatcc gcgcgcccgt cgttgccgcg gctttcgcgg ccccctgacc
      481 cttcccgttt gccgcgagat ccatgcacga gtatcgaacc cataattgcg gtgagctgac
      541 gctcgaccat gtcgggcggg aggtccggct gtcgggctgg gtgcaccgca agcgcgacca
      601 cggcaacctg ctgttcatcg atgtgcgcga tcgcttcggc cttacccagt gcgtggtcga
      661 gcagggcggc gcccatttcg aggcggtcgc ggcggtgcgc ccggaaagcg tggtgacagt
      721 gaccggcaag gcggtggcgc gcagcgagga cacggtcaac ccacgcctcg ccaccggcca
      781 tgtcgaggtg cgcatcgccg acttcgcggt ccagtcggcg gccgaggtgc tgccgatgca
      841 ggtcgcgggc gagcaggact attccgagga gatgcgcctg cgctaccggt tcctggactt
      901 gcgccgcgag cacatgcagc gcaacatcag gctgcgcgcc gacatcatcg ccagcatccg
      961 ccggcgcatg atcgcccagg gcttcaccga gtttcagacc ccgatcctga cctcgtccag
     1021 ccccgagggc gcgcgcgact tcctggtgcc gagccggctg cacccgggca agttctacgc
     1081 cctgccccag gcgccgcagc agttcaagca gctgatcatg gtcgcgggct tcgaccgcta
     1141 cttccagatc gcgccctgct tccgcgacga ggacgcgcgc gccgaccgca gcccgggcga
     1201 gttctaccag ctcgacatgg agatgtcgtt cgcgacccag gaggacgtgt tcgccgcgat
     1261 cgagccggtc ctccacgggg tgttcgagga gttcgcaagc tggcgcgtct cgccgcggcc
     1321 gttcccgcgc atccccttcg ccgaggcgat gctcagatac ggcaccgaca agcccgacct
     1381 gcgcaacccg atcctgatca gtgacgtgac cgaggcgttc cgcatggagg gcgtcgagtt
     1441 caaggcattc cgcaccgcca tcgacagggg cgcggtggtg cgcgcgatcc cggcgccggg
     1501 cgccgccgag cgcccgcgca gcttcttcga caagctcaac gactgggcgc gcgcggaagg
     1561 cgcgcctggg ctcggctaca tcgtgttcga cgccgagggt gccggcaagg ggccgatcgc
     1621 caagttcgtg cccgccgcgg cgcaggccgc gatcctcgag gccgccggcg ccggcgcggg
     1681 cgacgcggtg ttcttcgtct gcatgacgcc cagcgaggcg gccgagttcg ccgggcgcgt
     1741 gcgcacccgc ttgggcgacg agctcgattt gctggagacg gacgtgttca ggttctgctg
     1801 gacggtcgac ttcccgctct acgagcccga cgaggagacc ggcggggtca agttcagcca
     1861 caacccgttc tcgatgcccc agggcgggct cgaggcgctc gagaccagcg acccgctcga
     1921 catccgcgcc ttccagtacg acatcgtctg caacggcgtc gagctgtcct cgggcgcgat
     1981 ccgcaaccac cgccccgaca tcatgatcaa ggcgttcgcc atcgccggct acgcggcgga
     2041 ggacgtcgag gcgcgcttcg gcggcatgct gcgcgcgttc cgcttcggcg ccccgccgca
     2101 cggcggcatc gcgcccggca tcgaccgcat cgtcatgctt ctcgcccacg agcccaacat
     2161 ccgcgaggtg atcctgttcc cgatgaacca gcaggccgag gacctgctga tgggcgcccc
     2221 cagcgaggtc gacgacaagc acctgcgcga gctccacatc cgcgtcgccc tgccgcccaa
     2281 ggcgaagcag gagtgagcgc cgccgctcag gcggcgtagg cgagcacctg cttggtgccc
     2341 tcgacgatca tcgatacggc gaccaccgca accaccgcca ggcccagata gccgagccaa
     2401 cgccagcgct cgatcaggcg cgcgaccagg ttggctgcga agcccatcag cgccaccgac
     2461 agcgccagcc cgaacaccag cacccagacg tgatgacgcg cggtgccggc gaccgcgagc
     2521 acgttgtcga gcgacatcga cacgtcggcg accacgatct gccagatcgc cgagcggaag
     2581 ctcgacaccg gccgcgcggg tgcggccgcg ccgggatcgg ctgcgcccgc ggcggccccg
     2641 gccggcgccg gcacgcgcga tgcctcgagc tcgcgccaca tcttccacgc cacccacaac
     2701 agcagcagcc cgccggcgag caccaggccg atgatcgcga gcagggagct tgccaccacc
     2761 gcgaaggcga tcctgagcac catcgccgcg agcacgccga acaggatcgc gcgcgggcgc
     2821 agctcggccg gcaggcgcgc cgcgaccgcg ccgaccacga tcgcgttgtc gccggcgagc
     2881 accaggtcga ccaggatcac cgaggcgagg gcgaccagct cgccgctcag gacgtcgtag
     2941 accggcatgg ctcctc
//
LOCUS       DASWHU010000165         5314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1935987, whole genome shotgun sequence.
ACCESSION   DASWHU010000165 DASWHU010000000
VERSION     DASWHU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935987"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..660
                     /gene="pcaG"
                     /locus_tag="VGB88_06250"
     CDS             70..660
                     /gene="pcaG"
                     /locus_tag="VGB88_06250"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX9647080.1"
                     /translation="MGHGQTPSQTVGPFFAYCLTSDQYGYPFRGLAGPAMAGEECAGE
                     HIRLVGRVLDGDGKPIDDAVIELWQADSRGRYAHPADARGTNAPFAGFGRCGTGTDPE
                     RRFAFDTIKPGAIGDGQAPHLNLVVFMRGLLSHLYTRAYSSDVAAANAADPVLATVAA
                     ERRATLLAERAQTARGVVYRFDIRMQGEHETVFFDV"
     gene            676..1998
                     /gene="pcaB"
                     /locus_tag="VGB88_06255"
     CDS             676..1998
                     /gene="pcaB"
                     /locus_tag="VGB88_06255"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX9647081.1"
                     /translation="MTVSALESAITGMLLSDAETAALLSDGAHIDAMLRVEAALARAE
                     AEAGAIPAQAARRIAEVAATLAVAPESLAAGTAEAGVPVAALAAKLRQAVGADAADFV
                     HWGATSQDVIDTALVLRLGTVLALFETRIKGLCAALADLAEKHRDTAMAARTRSQQAV
                     PTSFGLKAAGWRAPLVRDLARLAELRPRLLVVSLGGAGGTLAALGDAGLAVEAAFARD
                     LALGVAPLPWHTQRDAICELAGWLALVTGSLGKMADDIILLAQSEVGEVRAGAGGGSS
                     TMPNKANPILPEALVALARFNAAQAGAVHQAMLQAHERAGPGWQLEWLTVPQMALATG
                     AALRHAAAILGALRVDPARMRANLDASNGLVLAEAASFALARHMPRSQARALVEDACR
                     EARAGGGHVIDILAKRCAAPVDWRALKDPANYLGAAAALIERALKETP"
     gene            1995..3650
                     /locus_tag="VGB88_06260"
     CDS             1995..3650
                     /locus_tag="VGB88_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9647082.1"
                     /translation="MTGPISGGEAIIEAVARHGADTVFGLPGAQIYPLFDALERAGGR
                     IATVTCRHEQAAGYMAFGYAASTGRPGVYAVVPGPGVLNTSAALCTAAGCNAPVLCLT
                     GEVPHEFLGQRRGHLHELPDQLATLRSLIKWAERIDKPGDAPRLVGEAFRHMLSGRRG
                     PVALEMCWDSMAATAEVSFPEPLAPDPAPEPDPDAVARAAKLLAGAAAPMIMVGGGAQ
                     AAAAEVLELAELLDAPVAAFRAGRGVVSEAHALGISSAAAYALWPETDVLLGIGTRLE
                     MPYMRWTGMMRYIARPAPPPELIRIDIDAEEMTRLVPQVGIVADAALGARALAAAVRR
                     IGRGRRGERARIAAAKAEARQRIEAVQPEIAYLDVIRDVLPRDGFFVEELCQVGFASY
                     YGFPVLRPRTYVTPGYQGTLGFGFPTALGVKVANPDKAVVSIAGDGGFMFGLQELATA
                     AQYGIAVVTLVFNNNSYGNVRRDQQRLFQGRVIGADLVNPDFVALAESFGVRAERVAS
                     PAALKPALARALDAGAPALIEVAIPKDSEASPWPFIHPEPPRR"
     gene            complement(3679..>5314)
                     /locus_tag="VGB88_06265"
     CDS             complement(3679..>5314)
                     /locus_tag="VGB88_06265"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9647083.1"
                     /translation="ERQQGRIVMSLRSWAVTRLAAAVAAAAIVAWTTVAPAQSGALDE
                     ATIADLQQGMASGKYTARSIVEAYLEQIRTVDRAGPTINSVLEVNPDALSLADQRDAE
                     RRDGKLLGPLHGIPVLLKDNIETADKLHTTAGSYALEGLPVAEDAFVAAQLRAAGAVI
                     LGKANMSVWAYFRSTRGTSGWSPRGGQTRNPYILTRSPCGSSSGSGAAVAANIVAVAI
                     GTETDGSITCPAAANSIVGIKPTMGLVGRSGVVPGAHSQDVVGPLARTVADAATVLSA
                     ITGVDERDPATMRARPANTDYTQFLDTNGLEGARIGVARNYFGYHEGIDALIERSIEA
                     MRQAGAVIVDPANVETAGKFGGCEVTTFFYELKDDLGSYLRGRSNAGPQSLADLIAFN
                     DGNKQKEMPFFQQEFFQLSEANGGPLTDQKYIDDRAECVRLAGPEGIDATLASNDVVA
                     IVAPTLGPIMPIDITLGDHLMPAWATAHWVASVAAVAGTPHISMPAGYINGIPVGISF
                     MGAAWSEPTLIKLAYAFEQATRYREPPKFLPTDPAP"
BASE COUNT          672 a         1927 c         1958 g          757 t
ORIGIN      
        1 atcgcgctcg gctacgtgtt cgacatcacc ttgcgcggcg gcgacgcgac gccgctggag
       61 ggctgagcga tggggcatgg ccagaccccg tcgcagaccg tcggcccgtt cttcgcctac
      121 tgcctgacct ccgaccagta cggctacccg ttccgcggcc tcgccgggcc ggcgatggcc
      181 ggcgaggagt gcgcgggcga gcacatccgg ctggtcgggc gcgtgctcga cggcgacggc
      241 aaaccgatcg acgacgcggt gatcgagctg tggcaggccg attcgcgcgg ccgctacgcg
      301 catcccgcgg atgcgcgcgg caccaacgcg ccgttcgccg gcttcggccg ctgcggcacc
      361 ggcaccgacc cggagcgccg cttcgccttc gacacgatca agccgggcgc gatcggcgac
      421 ggccaggcgc cgcacctcaa cctggtggtg ttcatgcgcg ggctgctgag ccacctctac
      481 acccgcgcct actcctccga cgtggcggcg gcgaacgccg ccgacccggt gctggcgacg
      541 gtggccgcgg agcgccgcgc gaccctgctc gccgagcgcg cgcagaccgc ccgcggcgtc
      601 gtctaccgct tcgacatccg catgcagggc gagcatgaga cggtgttctt cgatgtctga
      661 gcgccgccgg cgcggatgac cgtatcggct ctggagtccg cgatcaccgg catgctgctc
      721 tcggacgccg agaccgcggc gctgttgtcc gatggcgcgc acatcgatgc catgctacgg
      781 gtcgaggcgg cgctggcgcg cgccgaggcg gaggcgggcg cgatcccggc gcaagccgcg
      841 cgccggatcg ccgaggtcgc ggcgacgctc gcggtggcgc ccgagagcct cgccgccggc
      901 acggccgagg ccggagtccc ggtcgcggcg ctggcggcca agctgcgcca ggcggtcggc
      961 gcggacgcgg ccgatttcgt ccactggggc gcgaccagcc aggacgtcat cgacaccgcg
     1021 ctggtgctca ggctcggcac cgtgctggcg ctgttcgaga cgcgcatcaa gggcctgtgc
     1081 gccgcgctcg cggacctcgc cgaaaaacat cgcgatacgg cgatggcggc gcgcacccgc
     1141 tcgcagcagg cggtgccgac cagcttcggc ctcaaggccg cgggctggcg agcgccgctg
     1201 gtgcgcgacc tggcgcgcct cgccgagctc aggccgcggc tgctggtggt ctcgctcggc
     1261 ggtgcaggcg gcaccctggc ggcgctcggc gatgccgggc tcgcggtcga ggcggcgttt
     1321 gcccgcgacc tcgcactcgg cgtcgcgccg ctgccctggc acacccagcg cgatgcgatc
     1381 tgcgagctcg ccggctggct cgcgctggtc accggctcgc tcggcaagat ggccgacgac
     1441 atcatcctgc tcgcccagtc cgaggtcggc gaggtgcgcg cgggcgcggg cggcggctcc
     1501 tcgaccatgc ccaacaaggc gaacccgatc ctgcccgagg cgctggtcgc gctcgcccgc
     1561 ttcaacgccg ctcaagcggg cgccgtgcac caggcgatgc tgcaggcgca cgagcgcgcc
     1621 ggccccggct ggcagctcga gtggctgacc gtgccgcaga tggcgctcgc caccggcgcg
     1681 gcgctccgcc acgcggcggc gatcctcggc gcgctcaggg tggatccggc gcgcatgcgc
     1741 gccaatctcg acgcctcgaa cggcctggtg ctggccgagg cggcgagctt cgcgctcgcc
     1801 cgccacatgc cgcgcagcca ggcccgggcg ctggtcgagg acgcctgccg cgaggcgcgc
     1861 gcgggcggcg gccacgtgat cgacatcctg gcgaagcgat gcgccgcgcc ggtcgactgg
     1921 cgggcgctca aggacccggc caactatctc ggcgccgcgg cggcgctgat cgagcgcgcg
     1981 ctcaaggaaa caccatgacc ggtccgatca gcggcggcga ggcgatcatc gaggcggtgg
     2041 cgcgccacgg cgccgatacc gtgttcggcc tgcccggcgc ccagatctat ccgctgttcg
     2101 acgcgctcga gcgcgccggc gggcgcatcg ccaccgtcac ctgccggcac gagcaggcgg
     2161 ccggctacat ggcgttcggc tacgccgcct cgaccggccg gcccggggtc tatgcggtgg
     2221 tgccggggcc gggcgtgctc aacacctcgg cggcgctgtg caccgcggcc ggctgcaacg
     2281 cgccggtgct gtgcctgacc ggcgaggtgc cgcacgagtt cctgggccag cgccgcggcc
     2341 atttgcacga gctgcccgac cagcttgcga ccttacgctc gctgatcaag tgggccgagc
     2401 gcatcgacaa acccggggac gcgccgcggc tggtcggcga ggcgttccgc cacatgctgt
     2461 cgggacggcg cgggccggtg gcgctcgaga tgtgctggga cagcatggcg gcgacggcgg
     2521 aggtgtcgtt ccccgagccg ctcgcgcccg accccgcgcc cgagcccgat ccggacgcgg
     2581 tcgcccgggc ggcgaagctg ctcgccggcg cggccgcgcc gatgatcatg gtcggcggcg
     2641 gcgcgcaagc ggcggcggcc gaggtgctcg agctcgccga gctgctcgac gcgccggtcg
     2701 ccgcgttccg cgccgggcgc ggcgtggtct cggaagcgca cgcgctcggc atctcctcgg
     2761 ccgcggcgta cgcgttgtgg cccgagaccg acgtgctgct cggcatcggc acgcggctcg
     2821 agatgccgta catgcgctgg accggcatga tgcgctacat cgcccgcccg gcgccgccgc
     2881 ccgagctgat ccgcatcgac atcgacgccg aagagatgac gcggctggtg ccccaggtcg
     2941 gcatcgtcgc cgacgcggcg ctgggtgcgc gcgcgctcgc cgcggcggtc aggcggatcg
     3001 ggcgcggccg gcgcggcgag cgggcgcgga tcgccgccgc caaggccgag gcgcggcaga
     3061 ggatcgaggc ggtgcagccc gagatcgcct atctcgacgt gatccgcgat gtgctgccgc
     3121 gggacggctt cttcgtcgag gaactgtgcc aggtcggctt cgcgtcctat tacggcttcc
     3181 cggtgctccg cccccgcacc tacgtcaccc cgggctacca gggcacgctc ggcttcggct
     3241 tcccgaccgc gctcggggtc aaggtcgcca acccggacaa ggcggtggtg tcgatcgccg
     3301 gcgacggcgg cttcatgttc gggttgcagg agctcgccac cgcggcgcag tacggcatcg
     3361 ccgtggtgac tctcgtgttc aacaacaatt cgtacggcaa cgtccgccgc gaccagcagc
     3421 gcctgttcca gggccgggtc atcggcgccg acctggtcaa ccccgacttc gtcgccctcg
     3481 ccgagagctt cggcgtgcgg gctgagcgcg tcgcctcgcc ggccgcgttg aagcccgcgc
     3541 tcgcccgggc gctggacgcg ggcgcgccgg ccctgatcga ggtcgcgatt cctaaggaca
     3601 gcgaggcctc accgtggccg ttcatccacc ccgaaccgcc gcgccggtga gccccgcccg
     3661 gcggcggccg gcccgctatc agggcgccgg atcggtcggc aggaacttgg gcggctcgcg
     3721 gtacctggtc gcctgctcga aggcgtaggc gagcttgatc agggtcggct cgctccacgc
     3781 cgcgcccatg aacgagatgc ctacgggtat gccgttgatg tagccggccg gcatcgagat
     3841 gtgcggcgtg cccgcgaccg ccgccaccga ggccacccag tgcgcggtcg cccaggccgg
     3901 catcaggtgg tcgccgaggg tgatgtcgat cggcatgatc gggccgaggg tgggggcgac
     3961 gatcgccacc acgtcgttgc tggcgagcgt cgcgtcgatg ccttcgggcc ccgccaggcg
     4021 cacgcactcg gcgcggtcgt cgatatattt ctggtcggtc agcggcccgc cgttcgcttc
     4081 cgagagctgg aagaactcct gctggaagaa cggcatctcc ttttgcttgt tgccgtcgtt
     4141 gaacgcgatc aggtccgcga gcgactgcgg gcccgcgttc gaccggcccc tgagatagct
     4201 gccgaggtcg tccttcagct cgtagaagaa cgtggtcacc tcgcagccgc cgaacttgcc
     4261 cgcggtctcg acattggccg gatcgacgat caccgccccc gcttgccgca tggcctcgat
     4321 gctgcgctcg atcagggcgt cgatgccctc gtggtagccg aagtagttgc gcgcgacgcc
     4381 gatgcgcgcg ccctcgaggc cgttggtatc caggaactgg gtgtagtcgg tattggccgg
     4441 gcgtgcgcgc atggttgcgg gatcgcgctc atcgacgccc gtaatcgcgc tgagcacggt
     4501 cgcggcgtcg gcgacggtgc gcgcgagcgg cccgaccacg tcctggctgt gcgcgccggg
     4561 caccacgccc gagcgcccga ccaggcccat ggtcggcttg atgccgacga tcgagttggc
     4621 cgccgccggg caggtgatcg agccgtcggt ctcggtgccg atggcgaccg cgacgatgtt
     4681 ggccgcgacc gccgcgcccg agcccgagct cgacccgcac gggctgcggg tgaggatgta
     4741 ggggttgcgg gtctggccgc cgcgcgggct ccagccgctg gtgccgcggg tcgagcggaa
     4801 gtaggcccac acgctcatgt tcgccttgcc gaggatcacc gcgccggccg cgcgcagctg
     4861 ggcggcgacg aatgcgtcct ccgccaccgg cagcccctcg agcgcatacg agcccgcagt
     4921 ggtgtgcaac ttgtcggcgg tctcgatgtt gtccttgagc aggaccggga tgccgtgcag
     4981 cgggccgagc agcttgccgt cgcgacgctc ggcatcgcgc tggtcggcta ggctgagcgc
     5041 gtccgggttg acctcgagca ccgagttgat ggtcggcccc gcccggtcga ccgtcctgat
     5101 ctgctcgaga taggcctcga cgatggagcg cgcggtgtac ttgcccgacg ccatgccctg
     5161 ctgaaggtcg gcgatggtgg cctcgtcgag cgcgccggat tgcgccggcg caaccgttgt
     5221 ccaggcaacg atggccgcgg cggcgaccgc ggcggcgagc ctggtgacag cccatgaccg
     5281 taaagacatc acgatcctcc cctgctggcg ttcc
//
LOCUS       DASWHU010000166         6533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_812383, whole genome shotgun sequence.
ACCESSION   DASWHU010000166 DASWHU010000000
VERSION     DASWHU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812383"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="VGB88_06270"
     CDS             complement(<1..2011)
                     /locus_tag="VGB88_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647084.1"
                     /translation="MRQRRSAAGVALAVGLAICAALPAAANGVTVSDLLRPAPRQPVG
                     AYDQFGELLTPLAAFGRVWQEGLNPFLPASYQRLGMVRIGALQILRGEVIFFNEEIGD
                     QLGVQRLFGVRLALYDLLPELLTAIVALRGRPTTNLALVLNRDWTIGTHEFPRGTVIK
                     SGLDVEAGAWLPIGVRLDGNITCALCHVTLDARTGKRLDGVPNSDLDVAFMLAFAKNS
                     AAAFARLDIDPFDAAFLGNGKTIIDSAGQPVELPDPEAFETAFDDLILDVPPGQFESS
                     PDRINNTTQIPSLFTFRSGPYGFDGVFGVGPFAGLAALTNAVHTSEINLLAAAQTSAA
                     LLGIDPEAYLGIALQNAVDPGLRLPGGAPVRPSEWLRQVAPDLALAELEDQVEAPGAG
                     QYPALQPSLFALSGLIFSPRTASLDFATGRFMGAVNAMSAYQNSLVPPPNRSLANLIA
                     LVDGSVARGAQAFLDAGCAGCHAPPFYTDNAIHPLAAIGSNPARAMSRLGVEPLLVPP
                     MLYSFDTPVPIPADAELLEVPTAGFSATPTSLPDGLAPAGGYRTTSLRGIAFSAPYLH
                     DGGVAVRAGALSYPGDGSFAIADPAGLGVPGTLAQGINADAAASLRALLDRTLRAAVI
                     AANQASPGLVRSNLDGTGHAFWADAAAGFTPAEQTDLIDFLLALDD"
     gene            complement(2092..2694)
                     /locus_tag="VGB88_06275"
     CDS             complement(2092..2694)
                     /locus_tag="VGB88_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX9647085.1"
                     /translation="MARLARQLVIFVKAPRRGAVKTRLARDIGAGAAQRFARACARET
                     TRRLARDPRWRCWLAVTPDRFARTGRFWPNGPARLAQGPGDLGARMARPLLRLPPGPV
                     VRVGSDIPALRADHVARAFRALGRAEAVFGPAADGGYWLIGLSRRPALRIPFRGVAWS
                     TGAALAETRARLPRAARVAYLETLEDIDDAAAWRRWRAGT"
     gene            complement(2710..3387)
                     /locus_tag="VGB88_06280"
     CDS             complement(2710..3387)
                     /locus_tag="VGB88_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX9647086.1"
                     /translation="MDERAISAVIPTLNAGATLAATLDALAGAVGEVVVADGGSTDDT
                     AALARARGARVVEAPAGRGAQLAAGARAATGDWLLFLHADTVPLAGWRRQVARFIAAP
                     ANRERAAAFGYATDLAGAAGRWLEAYVAWRTRALGLAYGDQGLLISRAFYDRLGGFRP
                     LPIMEDVDLVRRIGRRRLVALDARARTSGARYRRAGVAVRGARNLACLALYFLGVPPR
                     LIVRLYG"
     gene            3614..4684
                     /locus_tag="VGB88_06285"
     CDS             3614..4684
                     /locus_tag="VGB88_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX9647087.1"
                     /translation="MAGMVPREARKGRGAASNRAGRYEPHGREAADDGWGALDEPQPP
                     LRTTVAIDASRTIIARNTSPDVPFDRSINAYRGCEHGCVYCFARPTHAFLGLSPGQDF
                     ETRLFAKPDAPRLLRDELARPGYRCEPIALGTNTDPYQPIERRFNITRGILEVLLEHR
                     HPVTITTKSALVTRDLDILGEMAELGLAKAFLSVTTLDRRLANRLEPRASTPDKRLEA
                     IRALAAAGVPVGVMVAPVIPGLTDPELESILLAAARAGAREAGWLLLRLPLEVRGLFE
                     EWLAVHAPAKAKRVLALVRETRGGRMNDPRFGHRHRGEGAYAELIGQRFAAAVRRLGL
                     NLRTQALDTTRFKPPREQMSLF"
     gene            4719..5351
                     /locus_tag="VGB88_06290"
     CDS             4719..5351
                     /locus_tag="VGB88_06290"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9647088.1"
                     /translation="MAGERGPDYAWEAAVPAPVCGVDEAGRGPLAGPVVAAAVILAPG
                     RIPAGIDDSKRLAPARRAALDAALRRDAEVALGIASVDEIDILNVLQASLLAMARAVA
                     GLPRPPASALIDGTFAPALACPARALVRGDARSLSIAAASIVAKCARDRIMAELALSF
                     PGYGWETNAGYGTPAHLAALARLGPSPHHRRGFAPVRAAAGLARGTGRPI"
     gene            5499..>6533
                     /locus_tag="VGB88_06295"
     CDS             5499..>6533
                     /locus_tag="VGB88_06295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9647089.1"
                     /translation="MAAMPAASVDLVFADPPYNLQLREGLRRPNHSLVDGVAEDWDRF
                     DGFADYDRFTRAWLAAARRVLKPTGTLWVIGTYHNIFRIGTALQDLGFWVLNDIVWRK
                     TNPMPNFRGRRFTNAHETLIWCKRSEDQARYTFNYDAMKALNDELQMRSDWLIPLCAG
                     PERLRAGGPACAEAKPLRLRASRRKAHPTQKPEALLHRVILASTNAGEVVLDPFFGSG
                     TTGAVAKRLGRRYLGIEREDAYVALAAERLKSVRAGDDEAVAVTPSKRAQPRVPFGAL
                     IERGLIRPGTVLSDATRRHAARVRADGSLIAGEVSGSIHSVAAAIQGAPACNGWAFWF
                     FEAKGKLVPID"
BASE COUNT          898 a         2421 c         2403 g          811 t
ORIGIN      
        1 cgtcgtcgag ggcgagcagg aagtcgatga ggtcggtctg ctcggccggg gtgaagccgg
       61 cggcggcgtc ggcccagaag gcgtggccgg tgccgtcgag gttgctcctg accaagcccg
      121 gcgaggcctg gttggcggcg atgacggcgg cgcgcaaggt ccggtcgagg agcgcgcgca
      181 ggctcgccgc ggcatcggcg ttgatgccct gcgccaaggt cccgggcacg ccgaggcccg
      241 cggggtcggc gatcgcgaag ctgccgtcgc cggggtagct cagcgcgccc gcgcgcaccg
      301 cgacgccgcc gtcgtggagg tagggcgcgc tgaacgcgat gccgcgcaag ctcgtcgtcc
      361 ggtagccgcc ggccggcgcg agcccgtcgg ggagcgaggt cggagtcgcg ctgaagcccg
      421 cggtcggcac ctcgagcagc tcggcgtccg ccggtatcgg caccggggtg tcgaagctgt
      481 acagcatcgg cggcaccaag agcggctcga cgccgagccg cgacatcgcc cgcgccgggt
      541 tcgagccgat cgcggccagc ggatggatgg cgttgtcggt atagaacggc ggcgcgtggc
      601 agccggcgca gccggcgtcg aggaaggcct gcgcgccgcg cgcaaccgag ccgtcgacca
      661 gcgcgatcag attggcgagc gagcggttgg gcggcggcac caggctgttc tgatacgccg
      721 acatggcgtt gaccgcgccc atgaaacggc cggtggcgaa gtcgaggctc gcggtcctcg
      781 ggctgaagat cagcccgctc aacgcgaaca ggctcggttg cagcgccggg tactgcccgg
      841 ccccgggcgc ctcgacctgg tcctcgagct cggcaagggc gaggtccggc gcgacctggc
      901 gcagccattc ggacgggcgc accggcgcgc cgccgggcag gcgcaagccg ggatcgaccg
      961 cgttctgaag cgcgatgccg agataggcct cagggtcgat gccgaggagt gcggcgctgg
     1021 tctgggccgc ggcgagcagg ttgatctcgg aggtatgcac ggcgttggtc agcgcggcga
     1081 gcccggcgaa cgggccgacg ccgaacacgc cgtcgaaccc gtacgggcct gagcggaagg
     1141 tgaacaggct cgggatctgg gtggtgttgt tgatgcggtc gggcgagctc tcgaactggc
     1201 ccggtggcac gtcgaggatg aggtcgtcga acgcggtctc gaacgcctcc gggtcgggca
     1261 gctcgaccgg ctggccggcg ctgtcgatga tcgtcttgcc gttgccgagg aaggcggcat
     1321 cgaacgggtc gatgtcgagg cgcgcgaacg ccgcggcgct gttcttggcg aaggcgagca
     1381 tgaaggccac gtcgaggtcg ctgttgggca ccccgtcgag ccgcttgccg gtgcgcgcgt
     1441 ccaaggtcac gtggcacagc gcgcaggtga tgttgccgtc gaggcgcacc ccgatcggca
     1501 gccacgctcc cgcctcgacg tcgaggccgg acttgatcac cgtgccgcgc gggaactcgt
     1561 gggtgccgat cgtccagtcc cggttcagca ccagcgcgag gttggtggtc gggcggccgc
     1621 gcagcgcgac gatcgcggtc aagagctcgg gcagcaggtc gtagagcgcg aggcgcacgc
     1681 cgaacagccg ctgcaccccc agctggtcgc cgatctcctc gttgaagaag atcacctcgc
     1741 cgcgcaggat ctgaagcgcg ccgatgcgga ccatgccgag ccgctggtag gacgcgggca
     1801 ggaacgggtt gagcccctcc tgccagacgc gcccaaacgc cgcgagcggc gtcagcagct
     1861 cgccgaactg gtcgtacgcg ccgaccggct gccgcggcgc gggccgcagc aggtccgaga
     1921 cggtgacgcc gttggcggcc gcgggcaggg cggcgcaaat cgcgagcccg accgcgagcg
     1981 cgacacctgc cgccgaccgg cgctggcgca tgtctccctc cccttgccgg tcacgcgccc
     2041 ggcatcggca tggagtgtaa tcgccgcggc gggccaagag aacacgcgcc gtcacgtccc
     2101 ggcacgccag cgccgccacg cggcggcatc gtcgatgtcc tcgagggtct ccagataggc
     2161 gacgcgggcc gcgcgcggca gcctggcgcg ggtctcggcg agcgcggcgc cggtcgacca
     2221 ggcgacgccg cggaaaggga tcctgagcgc cgggcggcgg ctgagcccga tcagccagta
     2281 gccgccgtcg gcggcgggac cgaacactgc ctcggcgcgc ccgagggcgc ggaacgcgcg
     2341 cgcgacgtgg tcggcgcgga gcgcggggat gtcgctgccc accctcacca ccgggcccgg
     2401 cggcaggcgc agaagcgggc gcgccatgcg tgcgccgagg tcgccgggac cctgggcgag
     2461 gcgcgcggga ccgttcggcc agaacctgcc cgtgcgcgcg aaccggtcgg gggtgaccgc
     2521 gagccagcag cgccagcgcg gatcgcgggc gagccggcgg gtggtttcgc gcgcgcaggc
     2581 tcgcgcgaat cgctgcgccg cgccggcacc gatgtcgcgc gcgagccggg tcttgaccgc
     2641 gccccgccgc ggcgccttga cgaagatcac gagctggcgg gcgagccgcg ccatcgccgg
     2701 ctgattcgct cagccgtaca ggcggacgat caggcgcggc ggcacgccga ggaagtagag
     2761 cgccaggcag gcgaggttgc gcgccccccg caccgctacg cccgcgcgcc gatagcgcgc
     2821 gcccgaggtg cgcgcgcgcg cgtccagcgc caccaggcgc cggcggccga tccggcgcac
     2881 caggtccacg tcctccatga tcggcagcgg ccggaagccg ccgagccggt cgtagaacgc
     2941 gcgcgagatc agcagcccct ggtcgccgta agcgagcccg agcgcgcgcg tccgccaggc
     3001 gacataggcc tcgagccagc gcccggccgc gcccgcgagg tcggtcgcgt aaccgaacgc
     3061 ggccgcgcgc tcgcgattcg ccggcgcggc gatgaaccgg gcgacctgcc gccgccagcc
     3121 ggcaagcggc accgtgtcgg catgcaggaa caacagccag tcgccggtcg ccgcgcgcgc
     3181 gccggcggcg agctgcgccc cgcgcccggc cggcgcctcg accacgcgcg cgccccgcgc
     3241 ccgcgcgagc gccgccgtgt cgtccgtcga gccgccgtcg gcgaccacca cctcgccgac
     3301 cgcgcccgcc aaagcatcga gtgtcgccgc caaggtcgcg cccgcgttca aggtcgggat
     3361 gaccgcgctg atcgcccgct cgtccatcgc ccgcgagcct acaccggccc tcgcaaggcg
     3421 catgcgccgt aatttggcac gcggaggcgc ggaggccgcg gagggcgaca ggaggagcaa
     3481 gacatctcgg acgacagtgc cggcgctgtc gtcgaaacag ttttctcttc cccctcttct
     3541 ccgcgacctc cgcgcctccg cgtgactcag aacatgttct tgttttgttc gaaacctgcg
     3601 ctatactgcc ggcatggctg ggatggtgcc gagagaggcg cgcaagggcc gcggcgcggc
     3661 gagcaaccgc gccgggcgct acgagccgca cggccgcgag gcagcggacg acggctgggg
     3721 cgcgctcgac gagccgcagc cgccgctgcg caccacggtc gcgatcgacg ccagccgcac
     3781 catcatcgcc cgcaacacct cgcccgacgt gccgttcgac cgctcgatca acgcctatcg
     3841 cggctgcgag cacggctgcg tctactgctt tgcccggccg acccacgctt tcctcggcct
     3901 gtcgccgggc caggatttcg agacccgcct gttcgccaag cccgacgcgc cgcggctgtt
     3961 gcgcgacgag ctggcgcgcc ccggctaccg ctgcgagccg atcgcgctcg gcaccaacac
     4021 cgatccctac cagccgatcg agcgccggtt caacatcacc cgcggcatcc tcgaggtcct
     4081 gctcgagcac cgccacccgg tgaccatcac caccaagtcg gcgctggtca cgcgcgacct
     4141 cgacatcctg ggcgagatgg cggagctcgg cctcgccaag gcgttcctgt cggtgaccac
     4201 cctcgaccgc cgcctcgcca accggctcga gccgcgcgcg agcacgcccg acaagcggct
     4261 ggaggcgatc cgcgcgctcg ccgccgccgg cgtgcccgtg ggcgtgatgg tggcgccggt
     4321 gatcccgggg ctaaccgacc ccgagctcga atcgatcctg ctcgccgcgg cgcgcgccgg
     4381 cgcgcgcgag gcgggatggc tgctgctgcg cctgccgctc gaggtgcgcg ggctgttcga
     4441 ggaatggctt gccgtccacg ccccggccaa ggcgaagcgc gtgctggcgc tggtgcgcga
     4501 gacccgcggc gggcgcatga acgacccgcg cttcggccac cgccaccgcg gcgagggcgc
     4561 ctatgcggag ctgatcggcc agcgcttcgc cgccgccgtg cgcaggctcg gcctgaacct
     4621 ccgcacgcag gcgctcgaca ccacccggtt caagccgccc cgcgagcaga tgtcgttgtt
     4681 ctgaccggcg cgcagggcgg cggcctatac tcgccgccat ggcgggggag cgggggcccg
     4741 actatgcctg ggaggcggcg gtgccagcgc cggtgtgcgg cgtcgacgag gccgggcgcg
     4801 gcccgctcgc cggccccgtg gtcgccgccg cggtgatcct cgcgccggga cgcatccccg
     4861 ccggcatcga cgattcgaag cgcctcgcgc ccgcgcgccg cgccgcgctc gatgccgcct
     4921 tgcgccgcga cgccgaggtc gccctcggca tcgcctcggt cgacgagatc gacatcctca
     4981 acgtgctcca ggcgagcctg ctggcgatgg cgcgcgctgt cgccgggctg ccgcggccgc
     5041 ccgcgtcggc gctgatcgac ggcaccttcg cgcccgctct cgcctgcccg gcgcgcgccc
     5101 tggtgcgggg cgacgcgcgc tcgctgtcga tcgccgccgc ctcgatcgtc gccaagtgcg
     5161 cgcgcgaccg gatcatggcc gagctcgccc tgagcttccc cggctatggc tgggagacca
     5221 acgccggcta cggcacgccg gcgcacctcg ccgccctcgc ccgcctcggg ccgagccccc
     5281 accaccgccg cggcttcgcc ccggtacggg ccgcggccgg gctcgcccgc gggaccggtc
     5341 gccctatttg atctacttgc gattcaacgg gatacgggcg gactccgaga tcgagtctcc
     5401 gccctggggg cggcggggga atggccgggc ggacccagca tgtggcagtc gacgacgcgc
     5461 cctatgggcg catcctgcac ggcgactgca tcgcctgcat ggcggcgatg cccgcggcct
     5521 cggtcgacct cgtgttcgcc gacccgccct acaacctgca gctccgcgag gggctgaggc
     5581 gcccgaacca cagcctggtc gacggcgtcg ccgaggactg ggaccgcttc gacggcttcg
     5641 ccgactacga ccgcttcacc cgcgcctggc ttgcggcggc gcgccgggtg ctcaagccga
     5701 ccggcacctt gtgggtgatc ggcacctacc acaacatctt ccgcatcggc accgcgctcc
     5761 aggacctcgg cttctgggtg ctcaacgaca tcgtctggcg caagaccaac ccaatgccga
     5821 acttccgcgg ccggcgattc accaacgcgc acgagaccct gatctggtgc aagcggagcg
     5881 aggaccaggc gcgctacacc ttcaactacg acgccatgaa ggcgctcaac gacgagcttc
     5941 agatgcgctc ggactggctg atcccgctgt gcgcggggcc tgagcggctg cgcgcgggcg
     6001 ggcctgcctg cgccgaagcg aagccgcttc ggcttcgcgc aagcaggcgc aaggcgcacc
     6061 cgacccagaa gcccgaggcg ctgctgcacc gcgtgatcct cgcctcgacc aatgccggcg
     6121 aggtggtgct cgacccgttc ttcggctcgg gcaccacagg cgcggtggcg aagcggctcg
     6181 gccgccgcta cctcggcatc gagcgcgagg acgcttacgt cgcgctcgcc gccgagcggc
     6241 tcaagagcgt gcgcgcgggc gacgacgaag cggtcgcggt gacgccgtcg aagcgcgcgc
     6301 agccgcgcgt cccgttcggc gcgctgatcg agcgcggcct gatccgcccc ggcaccgtgc
     6361 tgtccgacgc cacccgccgc catgccgcgc gggtgcgcgc cgacggcagc ctgatcgcgg
     6421 gcgaggtgtc gggctcgatc cacagcgtcg ccgccgccat ccagggcgcg ccggcgtgca
     6481 acggctgggc gttctggttc ttcgaggcca agggcaagct ggtgccgatc gac
//
LOCUS       DASWHU010000167         9435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1179813, whole genome shotgun sequence.
ACCESSION   DASWHU010000167 DASWHU010000000
VERSION     DASWHU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179813"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGB88_06300"
     CDS             <1..399
                     /locus_tag="VGB88_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647090.1"
                     /translation="EITLEVAAAGAIDVRGVEDRLAQVFRNLIDNAISFSPPAGAITV
                     RLERRRDGAVVTVEDQGPGIPEGKTGAIFERFYSERPSGEKFGTHSGLGLSISKQIVD
                     AHHGTIRAENRPGDGPGTAAGARFIVWLPY"
     gene            complement(421..642)
                     /locus_tag="VGB88_06305"
     CDS             complement(421..642)
                     /locus_tag="VGB88_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEX9647091.1"
                     /translation="MTENQKLDTAPITPAALAALGMPFLAYVRPAVVDGVACYALHRA
                     DGGAIAVAETREVAFVLARQHDLEPVSVH"
     gene            complement(683..1153)
                     /locus_tag="VGB88_06310"
     CDS             complement(683..1153)
                     /locus_tag="VGB88_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX9647092.1"
                     /translation="MPRSSVFSNPLLLGFEPFEQVLERIAKGAGDGYPPYNIEQVDER
                     RLRITIAVAGFADDELSATVEDSQLVIEGRRHDDGAERTFLYRGIAARPFRRTFVLAE
                     GLEVTGAEFKNGLLRIDIDRPLAESRARTIEIRSAGSGRTQTRGPTDLKAAGGR"
     gene            1440..2402
                     /gene="msrP"
                     /locus_tag="VGB88_06315"
     CDS             1440..2402
                     /gene="msrP"
                     /locus_tag="VGB88_06315"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX9647093.1"
                     /translation="MLIRVPRGWELPESAVSPESALASRRQVLGGLAAGIGVGLGLGA
                     LAPAWAAEGPAADLYPFPRNEAFDAGRKLTDERLVQTYNNFYEFGSHKKVWKAAQKLR
                     TDPWTVAVDGMVEAPFEIGVEDLLRRMPVEERIYRHRCVEAWAMTVPWGGFPLAALVA
                     LARPLASASYVRFECFQDRDLAPGQRQAWFPWPYTEGLTIPEATSELAFIATGLFGKP
                     LEKQNGAPIRLVLPWKYGFKSIKSIVRVTFTDQRPATFWNTINDKLYGFWANVNPDVP
                     FPDWSQATEKLLGEGREVPTRIFNGYGEQVAHLYDDVKGENVFF"
     gene            2422..3846
                     /locus_tag="VGB88_06320"
     CDS             2422..3846
                     /locus_tag="VGB88_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9647094.1"
                     /translation="MTDALTPDLCVIGAGSAGLSVAAGASRMGARVVLIEKGRMGGDC
                     LNTGCVPSKALLAAAHAAAAVRGARRFGIDAGAPAVDFAAVHRHVHGVIEAIAPNDSV
                     ERFEGLGVTVLRVPARFTAPDEVRAGDAVVRARRFVVATGSAPLVPPVPGIEQVPYLT
                     NETVFDATACPAHLLVIGAGPIGVELAQAHRRLGAAVTMVEMARALPRDDPECARFAL
                     EALTAEGVVLHEQARVTAVEATAGGVALTVEGEGGRQRLEGSHLLVAAGRRPSVEGLE
                     LEAAGIAYDRRGIQVDAGLRTTNRKVFAIGDVAGPYQFTHMASYQAGIVIRRALFRLP
                     AKVDYRAVPWVTYTDPEVAQVGLTESEARARHGEVRVVRWPLADNDRAQAEARTDGLI
                     KVMTTCRGRVLGASIAGAHAGELVLPWGLAIARRLGIGAVASLIAPYPTLSEASKRAA
                     GEWYAPALFGPRTRVLVRLLAKLG"
     gene            3926..5326
                     /locus_tag="VGB88_06325"
     CDS             3926..5326
                     /locus_tag="VGB88_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9647095.1"
                     /translation="MTSTTSTRVPFWRSLSGRLLLFTALFVLIGEVLIFVPSIARFRL
                     VFLEERLDAGKLATLALEAAPDGHLDEALEQRLLAHAMVEVVVLRTPDRSMLMLSTKM
                     PPPVDHTYDLRRAQAMDLIAAAFRVLIEGPSPVIRVLNRSRVEPGTVVEVVMTQHELY
                     RAMVDFSGRILTLSIVLSLMVAGLVYLALHGLLVRPMRAITDNLIRFQGNPEDASTQI
                     EETRRRDEVGVVHRTLAEMQRALRGSLLQKTRLAALGAAMGKINHDLRNTLASAMVVS
                     DRLAESEDPTVRKVTPRLMAAIDRAVTLCRRTLDYAQSAEPELKPERLSLKKLVDDVG
                     DIVAMDVGESALRWANQVPDGLEVVADHVQLFRVLQNIAQNSVEAMAGGGEVRIEGRR
                     RAGRVEIDIADTGPGIPPRVREHLFQPFHGSARPGGSGLGLAIARENMRLHGGDIELV
                     ETGAAGTRFRLVLPDR"
     gene            complement(5334..7115)
                     /gene="ggt"
                     /locus_tag="VGB88_06330"
     CDS             complement(5334..7115)
                     /gene="ggt"
                     /locus_tag="VGB88_06330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9647096.1"
                     /translation="MTGPAPAPRRRSVCNGLALALALLGALPAAADQGRVQPEPGTGA
                     AARGPATAAHEMIAAANPYAAEAGAAVLAQGGSAVDAAIAAQMVLNLVEPQSSGVGGG
                     AFLVTYDAARGMVETFDGRETAPAAAAPALFVRPDGTLPGYLEALVGGRSVGVPGLLR
                     MLEAAHRAHGRLAWRTLFEPAIALAEQGFPISPRLAALAGRVPTLARFADTAGYFLGP
                     DAMPKPAGTVLRNPELAATFRHIAEGGAEAFYHGDIAADIAAAVAGAPVNPGLLAESD
                     LAAYRARQRPPVCLTYRGYRVCGMGPPSSGGVAVLQILGLLSRFDLAALAPDSVEAVH
                     LFAEASRLAFADRDRYLADPDFVAVPTWGLLDPGYLAARAALIDTRRAAPGRAAGTPP
                     GARQAWQSGAAPEFAATSHLSVVDRDGNAAALTTSIEFAFGSALMVRGFLLNNQLTDF
                     SFQPERDGRPVANRVEAGKRPLSSMAPTVVFDPEGRLALVVGSPGGSRIICYVAKTIV
                     AVIDWGADVQRAIDQPNRCNRGGATELEADTALAALSEALAARGHEVEVVDMNSGLHA
                     IAVVRAGAVPALAGGADSRREGRAVGR"
     gene            7219..8124
                     /gene="panC"
                     /locus_tag="VGB88_06335"
     CDS             7219..8124
                     /gene="panC"
                     /locus_tag="VGB88_06335"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9647097.1"
                     /translation="MAGHKAATVGSGRELNEVATVAELRRRIAAWREAGETVALVPTM
                     GFLHRAHLSLIGIAHARCTRVITSIFVNPAQFGAGEDFASYPRDPEGDIAQLRAAAVD
                     IAFVPALEEVYPPGFATKVAVAGLTDGLCGPRRPGHFDGVATVVAKLLIQSLLDVAVF
                     GEKDYQQLQMIRRMTRDLDLPVAIAAAPTVREADGLALSSRNVYLSAAERALAPALFR
                     ALGDSQAALARGMPVEDACRDAAARLAASGFDPVDYVEVCDSETLEPVERLDRPARIL
                     AAAWLGRTRLIDNVPAAGARAGQRT"
     gene            complement(8112..9287)
                     /locus_tag="VGB88_06340"
     CDS             complement(8112..9287)
                     /locus_tag="VGB88_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647098.1"
                     /translation="MLKICRPAAPVVAALLLGGCSFAEEGLWPSLTGEEPSGAAQAPA
                     AESIEIAPSPAEASGQPLVAPSQTRMAAAGPATSQAVPLSMPAGTAVPNTGTFVGQKV
                     VSLSSELETLKGQIAAQSGEYQSVGNSAAQGSQRYHALVGDINARLQAGTTPGNPVMV
                     SQWQSAQQELDTVSSDSSKLTELSNRIAANSDMINYLFESTRAAYSIQGAVEEDHRQL
                     AILEDDLDRVRVSNDRLLNEINATISRHNSYLFGERRNLTALALAISSGRAYGPSLAG
                     MASPLPPSQPMAGAATGPALVVIRFDRPDVEYEQALYEAVTAALDRRSDARFELVAVT
                     AMSGSPSEVAANSGEAKRNAERVMRSLMNMGLPADRVTMASTASDAVRTNEVQLYVR"
BASE COUNT         1249 a         3373 c         3506 g         1307 t
ORIGIN      
        1 gagatcacgc tcgaggtcgc ggccgcgggc gcgatcgacg tgcgcggggt cgaggaccgc
       61 ctggcccagg tgttccgcaa cctgatcgac aacgcgatct cgttcagccc gcccgccggc
      121 gccatcaccg tgcggctcga acgccggcgc gacggcgccg tggtcacggt cgaggaccag
      181 ggcccgggca tccccgaggg caagaccggc gcgatcttcg agcgcttcta cagcgagcgg
      241 ccctcgggcg agaagttcgg tacccattca gggttgggcc tgagcatctc caagcagatc
      301 gtggatgccc atcacggcac catccgcgcc gagaaccgcc ccggggacgg gcccggcacg
      361 gccgccggcg cccgcttcat cgtctggctg ccgtattgaa gcgcgggcgg ccgcaccgcc
      421 tcagtggacg ctgaccggct cgaggtcgtg ctggcgggcg agcacgaacg ccacctcgcg
      481 ggtctccgcc accgcgatcg cgccgccgtc ggcgcgatgg agcgcatagc aggcgacgcc
      541 gtcgaccacc gccggcctga cataggcgag aaagggcatg cccagcgcgg caagcgcggc
      601 gggcgtgatc ggggccgtgt ctagcttctg gttctcggtc atgtctccgt ctccattcgg
      661 ggccgcaagg ggtcgggcgc gctcagcgcc cgccggcggc cttgaggtcg gttggccccc
      721 tggtctgcgt gcgtccggac cccgcgctcc ggatctcgat cgtgcgcgcg cggctctcgg
      781 ccagcggccg gtcgatgtcg atccgaagca gcccgttctt gaactcggcc ccggtgacct
      841 cgagcccctc ggcgaggacg aaggtgcgcc ggaacggccg cgccgcgatg ccgcggtaaa
      901 ggaaggtgcg ctcggcgccg tcgtcgtgcc ggcggccctc gatcaccagc tggctgtcct
      961 cgaccgtcgc cgacagctcg tcgtcggcga acccggcgac ggcgatggtg atgcgcaacc
     1021 ggcgctcgtc gacctgctcg atgttgtagg gcgggtagcc gtcgccggcg cccttggcga
     1081 tccgctcgag cacctgctcg aacggctcga agccgagcag caacgggttg ctgaagacgg
     1141 acgacctagg catggcactc ctccgcgatg agcgagcgtc tgtcactcgg gcagcccgcg
     1201 gacccgcact tcggcatccg cgccgcccgg cggcccgatc tggcgccgcc ggcaagtatg
     1261 ttggcatcgc cgccgcgatt cacaagtaat atcggcgccc ccgggcggcg atgggcggcc
     1321 cgctcacggc cgctcacaag gcgcggcgca ggggcgaagc gaattgacgc cccggccgca
     1381 acggcacaat gttccgccga ccgattcacg cccccagggg gcgccgagtg gaggtggcca
     1441 tgctgatccg agtcccgcgc ggatgggagc ttcccgagtc cgcggtctcg cccgagtccg
     1501 ccctcgccag ccgccgccag gtgctgggcg ggcttgccgc cggcatcggc gtcggcctcg
     1561 gcctaggcgc gctcgctccg gcctgggccg ccgagggccc ggccgccgac ctctatccgt
     1621 tcccccgtaa cgaggcgttc gacgccgggc gcaagctgac cgacgagcgg ctggtccaga
     1681 cctacaacaa cttctacgag ttcggctcgc acaagaaggt ctggaaggct gcccagaagc
     1741 tgcgcaccga cccgtggacg gtggcggtcg acggcatggt cgaagcgccg ttcgagatcg
     1801 gcgtcgagga cctgttgcgc cggatgccgg tcgaggagcg catctatcgc caccgctgcg
     1861 tcgaggcgtg ggcgatgacc gtgccgtggg gcggcttccc gctcgccgcc ctggtcgcgc
     1921 tggccaggcc gctggcgtcg gcgagctatg tgcgcttcga atgcttccag gatcgcgacc
     1981 ttgccccggg ccagcgccag gcctggttcc cgtggcccta caccgaggga ctgacgatcc
     2041 ccgaggcgac cagcgagctc gccttcatcg ccaccggcct gttcggcaag ccgctggaga
     2101 agcagaacgg cgccccgatc cgcctggtgc tgccgtggaa gtacggcttc aagtcgatca
     2161 agtcgatcgt ccgcgtcacc ttcaccgacc agcgcccggc gacgttctgg aacacgatca
     2221 acgacaagct ctacggcttc tgggccaacg tcaatccgga cgtcccgttc cccgactgga
     2281 gccaggcgac ggagaagctg ttgggcgagg gccgcgaggt gccgacccgc atcttcaacg
     2341 gctacggcga gcaggtcgcc cacctctacg acgacgtcaa gggcgagaac gtcttcttct
     2401 gaggccggcc ccgcagccgc catgaccgac gcgctgaccc ccgatctgtg cgtgatcggc
     2461 gcgggctcgg ccgggctcag cgtcgccgcc ggcgcgagcc ggatgggcgc gcgcgtggtg
     2521 ctgatcgaga agggccggat gggcggcgac tgcctgaaca ccggctgcgt gccgtcgaag
     2581 gcgctgctcg ccgccgccca tgccgccgcc gcggtgcgcg gcgcgcgccg gttcggtatc
     2641 gacgcgggcg cgcccgcggt cgacttcgcc gccgtccacc gccacgtcca cggcgtcatc
     2701 gaggcgatcg cgcccaacga ctcggtcgag cgcttcgagg gcctgggcgt gaccgtgctg
     2761 cgcgtgccgg cgcgcttcac tgcgcccgat gaggtgcggg ccggtgacgc ggtcgtgcgc
     2821 gcgcggagat tcgtggtcgc gaccggctcg gcgccgctgg tgccgccggt cccgggaatc
     2881 gagcaggtcc cctacctgac caacgagacg gtgttcgatg ccaccgcctg ccccgcgcac
     2941 ctgttggtga tcggggcggg gccgatcggg gtcgagctcg cccaggcgca ccgccgcctg
     3001 ggcgcggcgg tgaccatggt cgagatggcg cgcgcgctgc cgcgcgacga ccccgaatgc
     3061 gcccgcttcg cgctcgaggc gctaaccgcc gagggcgtgg tgctgcacga gcaggcgcgg
     3121 gtgacggcgg tcgaggccac agccggcggc gtcgcgctca cggtcgaggg cgagggcggg
     3181 cgccagcgcc tcgaaggctc gcacctgctg gtcgccgccg gccgtcggcc gagcgtcgag
     3241 ggcctcgagc tcgaggccgc cggcatcgcc tacgaccgcc gcggcatcca ggtcgacgcc
     3301 gggctgcgca ccaccaaccg caaggtgttc gcgatcggcg acgtcgccgg gccctaccag
     3361 ttcacccaca tggcgtccta tcaggccggc atcgtcatcc gccgcgccct gttccggctg
     3421 ccggcgaagg tcgactaccg ggcggtgccg tgggtcactt acaccgatcc cgaggtcgcc
     3481 caggtcgggc tgaccgagag cgaggcgcgg gcgcggcacg gcgaggtccg ggtggtgcgc
     3541 tggccgctcg ccgacaacga ccgggcgcag gccgaggcgc gcaccgacgg cctgatcaag
     3601 gtgatgacca cctgccgcgg ccgcgtgctc ggcgcctcga tcgccggcgc ccatgccggc
     3661 gagctggtgc tgccgtgggg gctcgcgatc gcgcgccggc tcggcatcgg cgcggtggcg
     3721 tcgctgatcg cgccttaccc gaccctgtcg gaagcgagca agcgcgccgc cggcgagtgg
     3781 tacgcgccgg cgctgttcgg gccgcgcacg cgcgtgctgg tccggcttct ggccaagctc
     3841 ggctgagccg ccggcgccaa atccgctagg atggcgcgcg cgccggcgcg caggcttggc
     3901 aataaagatc ggggacgcct tgcgcatgac gtcgaccacg tcgacccggg tgccgttctg
     3961 gcgcagcctg tcggggcggc tgctgctgtt caccgccctg ttcgtgctga tcggcgaggt
     4021 gctgatcttc gtgccctcga ttgcccgctt ccggctggtg ttcctggagg agcgcctgga
     4081 cgccggcaag ctcgccaccc tggcgctcga ggccgccccc gacggccacc tggacgaggc
     4141 gctcgagcag cgcctgctcg cccacgccat ggtcgaggtg gtggtgctgc gcacccccga
     4201 ccgctcgatg ctgatgctca gcaccaagat gccgccgccg gtcgaccaca cctacgacct
     4261 gcgccgggcc caagccatgg acctgatcgc agcggcgttc cgggtgctga tcgagggccc
     4321 gagcccggtg atccgggtgc tgaaccggtc gcgggtcgag cccggcacgg tggtcgaggt
     4381 ggtgatgacc cagcacgagc tgtaccgggc gatggtcgat ttctcgggcc gcatcctgac
     4441 gctctcgatc gtgctgtcgc tgatggtcgc ggggctggtg tatctcgccc tgcacgggct
     4501 gctggtccgc ccgatgcgcg cgatcaccga caacctgatc cgcttccagg gcaatcccga
     4561 ggacgcctcg acccagatcg aggagacgcg gcgccgcgac gaggtcggcg tggtccaccg
     4621 gaccttggcg gagatgcagc gggcgctgcg cgggtcgctg ctgcaaaaga cgcggctcgc
     4681 cgccctcggc gccgcgatgg gcaagatcaa ccacgacctg cgcaacacgc tggcctcggc
     4741 gatggtggtc tcggaccggc tcgccgagag cgaggacccg accgtgcgca aggtgacgcc
     4801 caggctgatg gcggcgatcg accgcgccgt gaccctgtgc cggcgcaccc tcgactacgc
     4861 ccagtcggcc gagcccgagc tcaagccgga gcggctgtcg ctcaagaagc tggtcgatga
     4921 cgtgggcgac atcgtcgcca tggacgtggg cgagtcggcg ctcagatggg ccaaccaggt
     4981 gcccgacggc ctcgaggtgg tcgccgacca cgtccagctg ttccgggtgc tgcaaaacat
     5041 cgcccagaac tcggtcgagg cgatggcagg cggcggcgag gtgcgcatcg aggggcggcg
     5101 tcgggcgggc cgggtcgaga tcgacatcgc cgacaccggg ccgggcatac cgccgcgcgt
     5161 gcgcgagcac ctgttccagc cgttccacgg ctcggcccgt cccggcggct cggggctcgg
     5221 cctcgccatc gcgcgcgaga acatgcggct ccacggcggc gacatcgagc tggtcgagac
     5281 cggcgcggcc ggcacccgct tccgcctggt cctgcccgac cgttgagcgc gcctcagcgc
     5341 ccgaccgccc ggccctcgcg gcgcgaatcg gcgccgccgg ccagcgccgg cacggcgccg
     5401 gcgcggacga ccgcgatcgc gtgcaggccg ctgttcatgt ccaccacctc gacctcgtgg
     5461 ccgcgcgcgg cgagcgcctc gctcagcgcc gcgagcgcgg tgtcggcctc gagctcggtg
     5521 gcgccgccgc ggttgcagcg gttgggctgg tcgatggcgc gctggacgtc ggcgccccag
     5581 tcgatcacgg cgacgatggt cttggcgacg tagcagatga tgcggctgcc gccgggcgag
     5641 ccgaccacga gggcaagccg gccctcgggg tcgaacacca cggtcggcgc catcgagctc
     5701 agcggccgct tgcccgcctc gacccggttg gcgacagggc ggccgtcgcg ctcgggctga
     5761 aacgagaagt cggtgagctg gttgttgagc aggaagccgc gcaccatcag cgcggagccg
     5821 aaggcgaact cgatgctggt ggtcagcgcc gcggcgttgc cgtcgcggtc gacgacgctg
     5881 aggtgcgagg tcgcggcgaa ctcgggcgcc gcaccgctct gccaggcctg gcgggcgccg
     5941 ggcggcgttc ccgcggcgcg gccgggtgcg gcgcggcggg tgtcgatcag ggccgcgcgc
     6001 gccgccaggt agccggggtc gagcagcccc caggtcggca ccgcgacgaa gtcgggatcg
     6061 gcgaggtagc ggtcgcggtc ggcgaaggcg agccggctcg cctcggcgaa caggtgcacc
     6121 gcctcgaccg agtccggggc gagcgcggcg aggtcgaagc gcgacagcag gccgaggatc
     6181 tgcagcaccg cgacgccgcc cgagctcggc gggcccatgc cgcagacccg atagccgcga
     6241 taggtcaggc acaccggcgg gcgctggcgc gcgcggtagg cggcgaggtc cgattcggcc
     6301 agcagcccgg ggttgaccgg ggcgccggcg accgcggcgg cgatgtcggc ggcgatgtcg
     6361 ccgtgataga acgcctcggc gccgccctcg gcgatatggc ggaaggtggc cgcgagctcg
     6421 ggattcctga gcaccgtgcc ggcgggcttg ggcatggcgt cggggccaag gaagtagccg
     6481 gcggtgtcgg caaagcgggc gagggtcggc acccgcccgg cgagcgcggc cagccgcggg
     6541 ctgatcggga agccctgctc ggcgagcgcg atcgccggct cgaacagcgt ccgccaggcg
     6601 agccggccgt gggcgcggtg cgcggcctcg agcatcctga gcagccccgg cacgcccacc
     6661 gagcggccgc cgaccagcgc ctcgaggtag cccggcaggg tgccgtcggg cctgacgaac
     6721 agcgcgggcg ccgccgccgc aggcgcggtc tcgcggccgt cgaaggtctc gaccatcccg
     6781 cgcgcggcgt cataggtgac caggaacgca ccgccgccga cgcccgagga ctgcggctcg
     6841 accaggttga gcaccatctg cgccgcgatc gccgcatcga cggcgctgcc gccctgggcc
     6901 agcaccgcgg cgccggcctc cgcggcgtag gggttggcgg ccgcgatcat ctcgtgggcg
     6961 gcggtggcgg ggccgcgggc ggcggcgccg gtgccgggct cgggctggac tcgcccctgg
     7021 tcggcggccg ccggcagcgc gccgagcagc gccagggcga gcgcgagccc gttgcaaacg
     7081 ctgcggcggc ggggggcggg cgcgggtccg gtcatggcgg cgctgctata gcacgcgcgc
     7141 aggccgttga cctgtgccga tgccgcgact aggtgtctgc cggccggcgc gggcggacgc
     7201 gggcaggaga cttgcaccat ggcggggcac aaggcggcaa cggtcggctc gggccgggag
     7261 ctcaacgagg tcgccaccgt cgccgagctg cgccggcgga tcgccgcctg gcgcgaggcc
     7321 ggcgagacgg tggcgctggt gccgaccatg ggcttcctgc accgcgcgca tctgtcgctg
     7381 atcgggatcg cgcacgcgcg ctgcacccgg gtgatcacct cgatcttcgt caacccggcc
     7441 cagttcggcg ccggcgagga cttcgccagc tacccgcgcg accccgaggg cgacatcgcg
     7501 cagctgcgcg cggcggcggt cgacatcgcc ttcgtgcctg cgctcgagga ggtctatccg
     7561 ccggggttcg ccaccaaggt ggcggtcgca ggcctgaccg atggcctgtg cgggccgcgc
     7621 cggccgggcc atttcgacgg cgtcgcgacg gtggtcgcca agctcctgat ccagagcctg
     7681 ctcgatgtcg cggtgttcgg cgagaaggac taccagcagc tccagatgat ccggcgcatg
     7741 acgcgcgacc tcgacctgcc ggtggccatt gccgcggcgc cgaccgtgcg cgaggcagac
     7801 ggcctggcgc tgtcgtcgcg caacgtctat ctgagcgcgg ccgagcgcgc cctcgcgccg
     7861 gcgctgtttc gagccctcgg cgattcgcag gccgcgctcg cccgcggcat gccggtcgag
     7921 gacgcgtgcc gcgacgccgc cgcccgcctc gccgcgagcg gcttcgatcc ggtcgactat
     7981 gtcgaggtct gcgacagcga gacgctggag cccgtcgagc gcctcgaccg gccggcacgc
     8041 atccttgccg cggcgtggct cgggcgcacg cgcctgatcg ataatgtgcc ggcggcgggc
     8101 gcgcgcgccg gtcagcgcac gtagagctgg acctcgttgg tgcgcacggc gtcgctggcg
     8161 gtcgaggcca tcgtcacccg gtccgcgggc aggcccatgt tcatcagcga gcgcatcacc
     8221 cgctcggcat tgcgcttggc ctcgccggaa ttggccgcga cctcgctggg cgagccgctc
     8281 atcgcggtca ccgcgaccag ctcgaagcgc gcgtcggagc ggcggtccag cgccgccgtt
     8341 accgcctcgt acagcgcctg ctcgtactcg acgtcggggc ggtcgaagcg aatcaccacc
     8401 agcgccgggc cggtggccgc gcccgccatc ggctgcgacg gcggcagcgg cgaggccatg
     8461 ccggccaggc tcgggccgta ggcgcggccg ctgctgatcg ccagcgcgag cgcggtcagg
     8521 ttgcgccgct cgccgaacag gtaactgttg tgccggctaa tcgttgcatt gatctcgttc
     8581 agaagccggt cgttcgacac ccgcacccgg tccaggtcgt cctcgaggat cgcgagctgg
     8641 cggtggtcct cctcgaccgc gccctggatg ctgtaggcgg cgcgggtcga ttcgaacagg
     8701 tagttgatca tgtcggagtt ggcggcgatc cggttcgaca gctcggtaag cttcgagctg
     8761 tccgaggaca ccgtgtcgag ctcctgctgg gcgctctgcc actggctgac catgaccggg
     8821 ttgcccggcg tggtgccggc ctgcaggcgc gcgttgatgt cgccgaccag ggcgtgatag
     8881 cgctgcgagc cctgcgcggc gctgttgccg accgactggt actcgccgga ctgggcggcg
     8941 atctggccct tgagcgtctc gagctcggag ctgagcgaga cgactttctg gccgacgaag
     9001 gtgccggtgt tgggcaccgc ggtgccggcc ggcatcgaca gcggcaccgc ctggctggtg
     9061 gccggcccgg cggcggccat gcgcgtctgg gacggtgcga ccagcggctg gccggaggcc
     9121 tcggcgggcg agggggcgat ctcgatcgat tcggccgccg gcgcctgggc tgctccggac
     9181 ggctcttccc ccgttaagga cggccacagt ccctcttccg caaacgagca gccgccgagc
     9241 aacagcgccg cgaccacagg cgcggcgggc ctacagatct tgagcatgat cgtgaaagcc
     9301 accctccgtg cacgcccgtg cccgggcatc cgctaaagta ttctgtattt tgtgctttga
     9361 gaccacccgc gattgggcgc gatcttacac cgaaggcttc cgcgctcaca aggcggtttt
     9421 tggccgaatc ctgcg
//
LOCUS       DASWHU010000168         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3392312, whole genome shotgun sequence.
ACCESSION   DASWHU010000168 DASWHU010000000
VERSION     DASWHU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392312"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VGB88_06345"
     CDS             complement(<1..1278)
                     /locus_tag="VGB88_06345"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX9647099.1"
                     /translation="MSTTTPAKAVVSNVPGAEETPRQYGIETLAVHAGARPDPYTGAR
                     STPIYQTTSFVFDDADHAASLFNLQTFGYIYSRMTNPTVAVLEERIAALEGGRGAVAA
                     ATGHAAQFLVAVTLLQSGDEFIASRNLYGGSVTQFGLSFARLGWKCHFVDPTNPENFR
                     TAMTDKVKFIFLECLANPGGIVVDIEAVAKIAHQFGVPLVVDNTLASPYLCRPMEWGA
                     DLVVHSTTKFIGGHGNTMGGVVVESGRFDWRQNDKFPTLAQPDPAYHGLTFAETFGDF
                     GFTMKVRAVALRDYGPTMSPTGAWYLLQGVETLPLRMDRHCANTLAVAEWLESHPKVS
                     WVSYSGLKSSPYYRLAQKYMPKGAGSVLTFGLKGGYQAGLKVVDAVDMFSHLANIGDT
                     RSLILHPASTTHRQLSAEQQQAAGAGPDVVRLSV"
     gene            complement(1283..1831)
                     /locus_tag="VGB88_06350"
     CDS             complement(1283..1831)
                     /locus_tag="VGB88_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9647100.1"
                     /translation="MVLPLARETADNPLIYLPRHDYYSDQKLRRILSDVRRFAMVGAS
                     TQWRRPSYYAMKYLTRKGYQVIPVNPARAGEEILGETVYASIGEVPGKLDMVDIFRSS
                     EEALEITKEVIANKDKKGIKYLWMQLTIRNDQAAELAEAAGLEVVMNRCPKIEYGRLS
                     GELSWCGINSRIVSARALKPPR"
     gene            complement(1889..2368)
                     /locus_tag="VGB88_06355"
     CDS             complement(1889..2368)
                     /locus_tag="VGB88_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9647101.1"
                     /translation="MLKAANAPDDATLRRVLEGARTIAVVGASSRWDRPSCRIMQYLQ
                     AVGYRIFPVNPNAAGGEILGRPVYASLAELPEPIDIVNVFRKPAAVGAVVAAAIAIGA
                     KAVWLQKGVAEARAAARARAAGLDVIEDRCIKTEHFRLFGGAGAAAAARLDSDPRAA"
     gene            complement(2369..>2887)
                     /locus_tag="VGB88_06360"
     CDS             complement(2369..>2887)
                     /locus_tag="VGB88_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9647102.1"
                     /translation="RLMVSITHLPKPVIARVHATATAAGCQLVATCDLAVASSAARFA
                     TPGVNIGLFCSTPMVALSRAVARKPAMEMLLTGEMISAEAAQALGLVNRVVAPEALDA
                     EVAALAREIAGKSPLTLAIGKEAFYRQVEMGLEDAYAYASRVMTENMMKRDAEEGIDA
                     FIEKRKPEWRGA"
BASE COUNT          416 a          986 c         1021 g          464 t
ORIGIN      
        1 gaccgagagg cggaccacgt cggggccggc gccggcggct tgctgctgct ccgcggacag
       61 ctggcggtgg gtggtcgagg ccgggtgcag gatcaggctg cgcgtgtcgc cgatgttggc
      121 gaggtgcgag aacatgtcga ccgcatcgac caccttcagc cccgcctggt agccgccctt
      181 gagcccgaag gtaagcaccg agcccgcgcc cttcggcatg tacttctggg cgaggcggta
      241 gtacgggctc gacttgagcc ccgagtacga cacccagctc accttcgggt ggctctccag
      301 ccactcggcg accgcgagcg tgttggcgca gtggcggtcc atgcggagcg gcagggtctc
      361 gaccccctgg agcaggtacc acgcgccggt cgggctcatg gtcgggccgt agtcgcgcag
      421 cgccaccgcg cgcaccttca tggtgaagcc gaagtcgccg aaggtctcgg cgaaggtcag
      481 cccgtgatag gccgggtcgg gctgggcgag ggtcgggaac ttgtcgttct ggcgccagtc
      541 gaagcggccc gactcgacga ccacgccgcc catggtgttg ccgtggccgc cgatgaactt
      601 ggtggtcgag tggaccacca ggtccgcgcc ccactccatc ggccggcaca gatagggcga
      661 ggcaagcgtg ttgtcgacca ccagcggcac gccgaactgg tgcgcgatct tcgccactgc
      721 ctcgatgtcg accacgatgc cgccggggtt ggcgaggcat tcgagaaaga tgaacttgac
      781 cttgtcggtc atcgccgtgc ggaagttctc gggattcgtc gggtcgacga aatggcactt
      841 ccagccgagc ctggcgaagc tcaggccgaa ctgggtgacc gagccgccgt agaggttgcg
      901 cgaggcaatg aactcgtcgc ctgactggag aagcgtcacc gcgaccagga actgggcggc
      961 atgaccggtc gccgccgcca ccgcgccgcg cccgccctcg agcgcggcga tgcgctcctc
     1021 gagcacggcc accgtcgggt tggtcatgcg cgagtagatg tagccgaagg tctgcaggtt
     1081 gaacagcgag gcggcgtggt cggcgtcgtc gaacacgaac gaggtggtct ggtagatcgg
     1141 cgtcgagcgc gcgccggtgt acgggtcggg gcgcgcgccc gcgtgcacgg cgagcgtctc
     1201 gatgccgtac tggcgcggcg tctcctcggc gcccgggacg ttggagacga ccgccttcgc
     1261 gggggtcgtg gtgctcatga tcctacctcg gtggcttgag cgcgcgcgcc gacacgatgc
     1321 gcgagttgat gccgcaccag ctcagctcgc cggacagccg gccgtactcg atcttggggc
     1381 agcggttcat caccacctcg agacccgccg cctcggccag ctccgcggcc tggtcgttgc
     1441 ggatggtgag ctgcatccac aggtacttga tgcctttctt gtccttgttc gcgatcacct
     1501 ccttggtgat ctcgagcgcc tcctcggacg agcggaagat gtcgaccatg tcgagcttgc
     1561 cgggcacctc gccgatgctg gcgtagaccg tctcgcccag gatctcctcg cccgcgcgcg
     1621 ccgggttgac cgggatcacc tggtagccct tgcgggtcag gtacttcatc gcgtagtagc
     1681 tcggccgccg ccactgggtg ctcgccccga ccatggcgaa acggcgcacg tcgctcaaga
     1741 tccggcgcag cttctggtcc gagtaatagt cgtggcgggg caggtagatg agcggattgt
     1801 ccgccgtttc ccgcgccagg ggcaaaacca tgtcgccgtt cctcttggct ggccggaggg
     1861 ctgtgtcggc tgggaagccg ggccactcct aggccgcacg cggatcgctg tcaagccgag
     1921 ccgccgcggc cgcgccggcg ccgccgaaca ggcggaaatg ctcggtcttg atgcaccggt
     1981 cctcgatcac gtcgaggccc gcggcacgcg ccctggcggc ggcgcgcgcc tcggccacgc
     2041 ctttctgcag ccacaccgcc ttggcgccga tcgcgatcgc cgccgccacc accgcgccga
     2101 ccgcggccgg cttgcggaac acgttgacga tgtcgatcgg ctcgggcagc tcggcgagcg
     2161 aggcgtacac cggccggccc aggatctcgc cgccggcggc gttcgggttg accgggaaga
     2221 tgcgatagcc caccgcctgg aggtactgca tgatccggca gctcggccgg tcccagcggc
     2281 tgctcgcgcc gaccaccgcg atggtgcgcg cgccctcgag aacccggcgc aaggtcgcgt
     2341 cgtccggcgc gttcgcggcc ttcagcatct aggcgccccg ccactcgggc ttgcgcttct
     2401 cgatgaacgc gtcgatgcct tcctcggcgt cgcgcttcat catgttttcg gtcatcaccc
     2461 ggctggcgta ggcgtaggcg tcctcgagac ccatctcgac ctggcggtag aacgcctcct
     2521 tgccgatcgc gagggtgagc ggcgacttgc cggcgatctc gcgcgcgagc gccgcgacct
     2581 cggcatcgag cgcctcgggt gcgaccaccc ggttgaccag cccgagcgcc tgcgcggcct
     2641 cggccgaaat catctcgccg gtcagcagca tctccatcgc cggcttgcgc gcaacggcgc
     2701 gactgagcgc gaccatcggc gtcgagcaga acaggccgat gttgaccccc ggggtggcga
     2761 agcgcgcggc gctcgaggcg accgcgaggt cgcaggtcgc gaccagctgg cagccggcgg
     2821 cggtggcggt cgcgtggacc ctggcgatca ccggcttggg caggtgggtg atcgagacca
     2881 tcagccg
//
LOCUS       DASWHU010000169         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2670045, whole genome shotgun sequence.
ACCESSION   DASWHU010000169 DASWHU010000000
VERSION     DASWHU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670045"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /gene="pgk"
                     /locus_tag="VGB88_06365"
     CDS             <1..198
                     /gene="pgk"
                     /locus_tag="VGB88_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9647103.1"
                     /translation="AHADNPIDIQEFMILPVGAASVADAMSHVSTGGGASLEFLEGKT
                     LPGVAALLPAGADITVPKEDA"
     gene            195..1481
                     /gene="eno"
                     /locus_tag="VGB88_06370"
     CDS             195..1481
                     /gene="eno"
                     /locus_tag="VGB88_06370"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9647104.1"
                     /translation="MTTIEDVIGREILDSRGNPTIEVDVFLEDGAVGTAMVPSGASTG
                     AHEAVELRDGDKKRYGGKGVLKAVEAVNTEIFDALSGLDAEDQIKIDRLMIELDGTPN
                     KSRLGANAILGVSLAVAKAAASDASLPLYRYVGGTSARLLPVPMMNIVNGGAHADNPI
                     DIQEFMILPVGAASVADAIRIGSEVFHALKRLLADDGHATAVGDEGGFAPNLASNDAA
                     ITYILKAVEAAGYKPGEDIALALDAAASELFRDGEYRLAGEDRRLGADEMIRYYEDLI
                     GRFPIVSIEDGLDEDDWDGWTALTAALGSRVQLVGDDVFVTNPGRLREGIARAAANAI
                     LVKVNQIGTLTETLETVETAHKAGWRAVISHRSGETEDTTIADLAVATNCGQIKCGSL
                     SRSDRLAKYNRLLRIEERLGRAGRYAGRAALARGRG"
     gene            1514..1840
                     /locus_tag="VGB88_06375"
     CDS             1514..1840
                     /locus_tag="VGB88_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9647105.1"
                     /translation="MAARGEARRRLRIVAVPTVLIGVALYLGYHTVQGERGLLAWLRT
                     NDQVASLRTDLAAARGERERLENRVVRLRPDSLDPDLLEERAQAVLNLGHRDDLVILE
                     PAPRVP"
     gene            2027..2953
                     /gene="pdhA"
                     /locus_tag="VGB88_06380"
     CDS             2027..2953
                     /gene="pdhA"
                     /locus_tag="VGB88_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX9647106.1"
                     /translation="MLMLRRFEEKAGQLYGMGLIGGFCHLYIGQEAVAVGIEQAISRD
                     DPVITSYRCHAHAIARGVEPRRVLAELTGRGAGISKGKGGSMHMFSPAHGFWGGHGIV
                     AAQVPIGTGIAFALKYRASDRVCITYFGDGAANQGQVFEAYNMAALWKLPVVYVIENN
                     QYAMGTSVKRATADAELYRRAEGFGIPGLPCDGMDVIAVRAAAREAVSFVRAGNGPMV
                     IEAKTYRYRGHSMSDPAKYRSREEVQQMREARDPIEQVKARLVESGSADDAALKEIDK
                     EVRAVINDAAEFAQNAPEPDPSELATDVTAGA"
     gene            2964..3790
                     /locus_tag="VGB88_06385"
                     /pseudo
     CDS             2964..3790
                     /locus_tag="VGB88_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
BASE COUNT          588 a         1286 c         1394 g          522 t
ORIGIN      
        1 gcccatgccg acaacccgat cgacatccag gagttcatga tcctgccggt gggcgccgcg
       61 agcgtcgccg acgccatgag ccacgtcagc accggcggcg gggcgtcgct cgagttcctc
      121 gagggcaaga ccctgcccgg cgtcgccgcg ctcctgcctg ccggcgccga catcacggtt
      181 ccgaaggagg acgcatgacc accatcgagg atgtcatcgg ccgcgagatc ctcgactcgc
      241 ggggcaatcc gaccatcgag gtcgacgtct tcctcgaaga cggggcggtc gggaccgcga
      301 tggtgccatc gggcgcctcg accggcgcgc acgaggcggt ggagctcagg gacggcgaca
      361 agaagcgcta tggcggcaag ggcgtgctga aggcggtcga ggcagtgaac accgagatct
      421 tcgacgcgct ctccggcctc gacgcagagg accagatcaa gatcgaccgg ctgatgatcg
      481 agctcgacgg cacgccgaac aaatcgcggc tcggcgccaa cgcgattctc ggtgtgagtt
      541 tggccgtcgc caaggcggcg gcgtcggacg ccagcctgcc gctctaccgc tatgtcggcg
      601 gcacctcggc gcggctgctg ccggtgccga tgatgaacat cgtcaatggc ggcgcccatg
      661 ccgacaaccc gatcgacatc caggagttca tgatcctgcc ggtgggcgcc gcgagcgtcg
      721 ccgacgccat ccgcatcggc tcggaggtgt tccatgcgct caagcgcctg ctcgccgacg
      781 acggccacgc gaccgcggtc ggcgacgagg gcggcttcgc gcccaacctg gcgagcaacg
      841 acgcggcgat cacctacatc ctgaaggcgg tcgaggcggc cggctacaag ccgggcgagg
      901 acatcgcgct cgcgctcgat gcggcggcga gcgagctgtt ccgcgacggc gagtaccggc
      961 tcgcgggcga ggaccgccgg ctcggcgcgg acgagatgat ccgctactac gaggacctga
     1021 tcggccgctt tccgatcgtc tcgatcgagg acggcctcga tgaggacgac tgggacggct
     1081 ggaccgcgct gaccgccgcg ctgggaagcc gcgtccagct ggtcggcgac gacgtgttcg
     1141 tcaccaatcc ggggcggttg cgcgagggca tcgcgcgcgc cgccgccaac gcgatcctgg
     1201 tcaaggtcaa ccagatcggc acgctcaccg agaccctgga gacggtcgag accgcgcaca
     1261 aggcgggctg gcgcgcggtc atctcgcacc gctcgggcga gaccgaggac accaccatcg
     1321 ccgacctcgc ggtggcgacc aactgcggcc agatcaagtg cgggtcgctg tcgcgcagcg
     1381 accggctggc caagtacaac cgcctgctca ggatcgagga gcgcctgggg cgggcagggc
     1441 gctacgccgg gcgcgcggcg ctggcgcgcg ggcgcggttg acggtcgcgg cccggcgtgg
     1501 ttcggtgagc gcgatggcgg cgcggggcga ggcgagacgg cggctgcgca tcgtcgcggt
     1561 gccgacggtc ctgatcgggg tcgcgctcta tctcggctac cacaccgtcc agggcgagcg
     1621 cggcctgctc gcctggctgc gcaccaacga ccaggtcgcg agcctgcgca ccgatctggc
     1681 cgcggcgcgg ggcgaacgcg agcggctcga aaaccgggtc gtgcggctgc gccccgacag
     1741 cctcgatccc gacctgctcg aggagcgggc gcaggcggtg ctcaacctcg gccaccgcga
     1801 cgacctggtg atcctggagc cggcgccgcg ggtgccgtga gggccgcccg gccgtgcttg
     1861 cctgagggcg ggtgaatccg tattttcgtc gcgcctgaat ggcgggtccg ggcgcggcaa
     1921 tccagcggga gtttcaatgg ctgccagcaa gccgatagcg aagctcgccc ccgcctcggc
     1981 agcgggcgag gtgagcgccg aggagctgct cgcgctctac cgcgacatgc tgatgctgcg
     2041 ccggttcgag gagaaggcgg ggcagctcta cggcatgggc ctgatcggcg gcttctgcca
     2101 cctctatatc ggccaggagg cggtcgcggt cggcatcgag caggcgatca gccgcgacga
     2161 cccggtgatc accagctacc gctgccacgc gcatgcgatc gcgcgcgggg tcgagccgcg
     2221 ccgggtgctg gccgagctga ccgggcgcgg cgccggcatt tccaagggca agggcggctc
     2281 aatgcacatg ttctcgcccg cgcacggctt ctggggcggg cacggcatcg tcgccgccca
     2341 ggtgccgatc ggcaccggca tcgcgttcgc gctcaagtat cgcgcctcgg accgcgtctg
     2401 catcacctat ttcggcgacg gcgccgccaa ccagggccag gtgttcgagg cctacaacat
     2461 ggccgcgctg tggaagctgc cggtggtcta cgtgatcgag aacaaccagt acgcgatggg
     2521 cacctcggtc aagcgcgcga ccgcggatgc cgagctctat cggcgcgccg aaggcttcgg
     2581 cattcccggc ctgccatgcg acggcatgga cgtgatcgcg gtgcgcgcgg cggcgcgcga
     2641 ggcggtgtcg ttcgtgcgcg ccggcaacgg cccgatggtg atcgaggcca agacctaccg
     2701 ctatcgcggc cactcgatgt cggacccggc caagtaccgc agccgcgagg aagtgcagca
     2761 gatgcgcgag gcgcgtgacc cgatcgagca ggtcaaggcg cgcctggtcg agagcggctc
     2821 tgccgacgac gccgcgctca aggagatcga caaggaggtg cgggcggtga tcaacgacgc
     2881 cgccgagttc gcccagaacg cgcccgagcc cgacccgtcg gagctcgcca ccgacgtcac
     2941 ggcgggggcg tgaggtggcc agcctgaccg tgcgcgaggc attgcgcgac gccatggccg
     3001 aggagatgcg gcgcgacgac gccgtgttcc tgatcggcga ggaggtcgcc gagtacgagg
     3061 gcgcctacaa ggtgagccag ggcctgctcg ccgagttcgg cccgatgcgg gtggtcgaca
     3121 ccccgatcac cgaggcggga ttcgccggcg tcggcgtcgg cgccgcgttc atgggcctgc
     3181 gcccgatcgt cgagttcatg accttcaact tcgccatgca ggcgatggac cagctgatca
     3241 actccgccgc caagacccgc tacatgtcgg gcgggctggt ggggtgcccg atcgtgttcc
     3301 gcggccccaa cgggccggcg gcgcgggtcg cggcgcagca ctcgcaggac tacgggtcgt
     3361 ggttcgcgca cgtgcccggg ctgatcgtga tcgcgcccca ttcggcggcc gacgccaagg
     3421 ggctgctgaa gtcggcgatc cgcgatccca acccggtgat gtttctcgag aacgagattc
     3481 tctacgggcg cagcttcgag gtccccgacg aggaggtgct gaccccgatc ggggtcgcgg
     3541 cgacggtgcg cgagggcgac gacgtgacgg tggtgagcta tgcgcgcggc atcgactacg
     3601 tgcacggcgc gctcgagcgc ctcgaggccg acggcatctc gcccgagatc gtcgacttgc
     3661 gcacgctccg cccgctcgac atcgacgcgg tggtcgcctc ggtgacgaag accaaccgcc
     3721 tggtggtggt cgaggagggc tggccggtcg ccggcatcgg cgccgagatc gccgcccagg
     3781 tgatggaccg
//
LOCUS       DASWHU010000170         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2670101, whole genome shotgun sequence.
ACCESSION   DASWHU010000170 DASWHU010000000
VERSION     DASWHU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670101"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VGB88_06390"
     CDS             <1..555
                     /locus_tag="VGB88_06390"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX9647107.1"
                     /translation="CAAAACNPCNPCNPCAAGAGAELTDAEARAAYDCAQPAMAAAYS
                     RSGVASASEFSAWTSYATGPYTSATHGGRYVINYANATGAAYGRYEQAGTLAAGSVLA
                     KPSFTVNKKGQVMVGPLFLMAKQDAGWSPDTLNWQYQMIMPDGSVFGTTNGAGSAKVQ
                     FCADCHNAMAEQDALMFLPDEYRR"
     gene            complement(651..1607)
                     /locus_tag="VGB88_06395"
     CDS             complement(651..1607)
                     /locus_tag="VGB88_06395"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9647108.1"
                     /translation="MRIAVIGAGGVGGYFGGRLAHAGEDVVFIQRGAHLEALQRSGLS
                     VSSPLGDFTVPKVRAQAGAAGIGPVDVVLVTVKSNHTGQAAALARDLLGPDTAVISLQ
                     NGIENEDRLAAVLGRAQVAGGVAYILSLIEAPGRIRHSGQTARMLFGELDGRPSARLE
                     AFRDACAQAGIDAELSADITAALWAKFAVICPHSGMTALTRMPIGPVRSDPDCRALLE
                     GCAREVIALAAARGVILPPALIENPCAFFDRVPADMTSSLHHDVTHGKPLELDWLNGA
                     VVRLAREAGLAAPVNHFIYAALKLHAGGHANGAAAEAAAPSR"
     gene            complement(1620..2162)
                     /locus_tag="VGB88_06400"
     CDS             complement(1620..2162)
                     /locus_tag="VGB88_06400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9647109.1"
                     /translation="MVRTFDALGAGGRAAAARRIEGICLASPDAGRTLEGAARDEFFG
                     LWAGGYLDRWPGLALVHGRDCESIDGYLVGCEDSAAAEQLYDAIFYYRAFAAHYRTYP
                     AHFHVNVAAEARNRGTGTALVEAFAGLCRARACPGMHLVTAADARNRAFYDRAGFAQV
                     ESKQVAGRDLVLLGRKLDQD"
BASE COUNT          334 a          860 c          800 g          306 t
ORIGIN      
        1 tgcgccgccg cggcgtgcaa tccctgcaat ccgtgcaatc cctgcgcggc cggcgccggc
       61 gccgagctga ccgatgccga ggcgcgcgcc gcctacgact gcgcgcagcc ggcaatggcc
      121 gccgcctaca gccgctccgg cgtggcgtct gccagcgagt tcagcgcctg gaccagctac
      181 gccaccggcc cctacacctc cgcgacccat ggcgggcgct acgtcatcaa ctacgccaat
      241 gcgaccggcg ccgcctatgg acgctatgag caggcgggca cgctggccgc ggggtcggtc
      301 ctcgccaagc cgagcttcac tgtcaacaag aagggccagg taatggtcgg gccactgttc
      361 ctgatggcca agcaggacgc cggctggagc cccgacacgc tcaactggca gtaccagatg
      421 atcatgcccg acggcagcgt gttcggcacc accaacggcg ccggctcggc caaggtccag
      481 ttctgcgccg actgccacaa cgccatggcc gagcaggacg ccttgatgtt cctgccggac
      541 gagtaccgcc ggtgacggcg gccggctgag ctttcccgcc cggcgcggga cccggttggg
      601 cggcggcgcg ccacacccgc cgtccaaggg gggcggtccc cgaccgtccc tcagcgactc
      661 ggggcggcgg cttcggccgc cgccccattc gcatgcccgc ccgcgtgcag cttgagcgcg
      721 gcgtagatga agtggttgac cggcgccgcg agcccggcct cgcgggcgag ccggaccacg
      781 gcgccgttga gccagtcgag ctcgagcggc ttgccgtggg tgacgtcgtg gtgcagcgac
      841 gatgtcatgt cggccggcac ccggtcgaag aacgcgcacg ggttctcgat caacgcaggc
      901 ggcaggatca cgccgcgcgc cgcggcgagc gcgatcacct cgcgcgcgca cccctccagc
      961 aacgcccggc agtcggggtc gctgcgcacc ggcccgatcg gcatccgggt cagcgcggtc
     1021 atgccgctgt gcgggcagat gaccgcgaac ttcgcccaca gcgcggcggt gatgtccgcc
     1081 gacagctcgg cgtcgatgcc ggcctgcgcg caagcgtcgc ggaacgcctc gagccgcgcg
     1141 ctgggcctgc cgtcgagctc gccgaacagc atgcgcgcgg tctgccccga atggcggatg
     1201 cggcccggcg cctcgatcag cgacaggatg taggcgaccc cgccggccac ctgcgcgcgg
     1261 ccgaggacgg cggcgaggcg gtcctcgttc tcgatcccgt tctggagcga gatgaccgcg
     1321 gtgtcagggc cgagcaggtc gcgggcgagc gccgccgcct gcccggtgtg gttgctcttg
     1381 accgtcacca gcaccacgtc caccgggccg atgccggccg cacccgcctg cgccctgacc
     1441 ttgggcacgg taaagtcgcc gagcgggctc gacacggaca gcccgctccg ttgcagcgct
     1501 tccagatgcg cgccccgctg gatgaacact acgtcctcgc cggcatgggc cagccggccg
     1561 ccgaaatagc cgccgacgcc gccggcgccg atcaccgcga tgcgcatgcc tccctcctcc
     1621 tagtcctggt ccaacttgcg gccgagcagg acgaggtcgc gcccggcgac ctgcttgctc
     1681 tcgacctgcg cgaatccggc gcggtcgtag aacgcgcggt tgcgcgcgtc ggcggcggtg
     1741 accaggtgca tgcccgggca ggcgcgcgcg cggcacaggc ctgcgaacgc ctcgaccagc
     1801 gccgtgccgg tcccccggtt gcgcgcctcg gctgcgacat tgacatggaa atgcgccgga
     1861 tacgttcggt agtgcgcggc gaacgcgcga tagtagaaga tggcgtcgta cagttgctcg
     1921 gccgccgccg agtcttcgca gcctaccaga tagccgtcta tcgactcgca gtcgcgaccg
     1981 tggacgagag cgagcccggg ccaacggtcg agatagccgc cggcccaaag gccgaagaat
     2041 tcatcgcgcg ccgcgccttc gagcgtcctg cccgcgtcgg gcgatgccag gcagatgcct
     2101 tcgatccgcc gcgcggccgc cgcccgcccg cccgcgccga gcgcgtcgaa cgtccgcacc
     2161 atcgccgcta ctcgaccgtg accgccttgg ccaggttgcg cggctggtcg acgtcggtgc
     2221 ccttgcacac cgcgacgtgg taggcgagca gctggaccgg gagcgcatac aggatcggcg
     2281 cgaccagcgg gtcgacctcg
//
LOCUS       DASWHU010000171         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3392929, whole genome shotgun sequence.
ACCESSION   DASWHU010000171 DASWHU010000000
VERSION     DASWHU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392929"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VGB88_06405"
     CDS             <1..227
                     /locus_tag="VGB88_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9647110.1"
                     /translation="IGGAVLDVWYSYPKADGPDVAPSRQPFHELDNVIMTPHASGWTE
                     GLIERRWTVIADNIGRLADGRPLHNRIARP"
     gene            319..1458
                     /locus_tag="VGB88_06410"
     CDS             319..1458
                     /locus_tag="VGB88_06410"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX9647111.1"
                     /translation="MIAATPAWALDEIVTPQVFEIADYATARGELIEGLRVGWEAYGK
                     LNLDKDNAILIVPYFSGTPHAAGKYDAADAQPGYWDAIIGPGKPLDTGTYYIVSADAL
                     CDVQVKDPHVTTTGPATIDPRTGERYGLDFPAVSIRDFVAVQKALLENLGIFKLRAVV
                     GFSMGAAQALQWAVAYPHMVERAILVTPWARADAWQIATIYAWTAPILRDPAWNGGAY
                     YGAPEPVEGLAAAYEVAFMDALGPAFLNATFGRKWAEDGNDPAAAPDNRFAVETWLQD
                     TARAYAETGDANHFLCQAKAIQLFEAGQRGDIAEALARVTAKTLLLPAAGDIILPHDL
                     SDEIAAQLTGAPAVERAVIPGGAGHMNGSTAIGEVGAAIARFLAE"
     gene            complement(1468..2394)
                     /locus_tag="VGB88_06415"
     CDS             complement(1468..2394)
                     /locus_tag="VGB88_06415"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011047338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9647112.1"
                     /translation="MKIGVVGVGAMGSVYAALLARGGHEVWAIDRWREHIEAIRAHGL
                     RVEGASGDHTVAVHATTDAAEVGPCELVVIATKAMDVAAAAKTAKALLGPDTPVLAIQ
                     NGLGSGEAVQGVVGPDRVVIGVAGGFGGSIIGPGHARHEGWELIRLGEIDGPVTPRLE
                     RIAEAWRAGGFKVKAFDDIHQLIWEKLICNISFSPVCTLTGLKVGAVVANADTFAVSA
                     GLAGEGYAVARAKGIRLDFDDPVAYVRAFALKIPDGRPSMLQDIEAGRRSEIDAINGG
                     VARTGREVGVATPVNDTCIALVKGREAALGLR"
     gene            complement(2451..>2894)
                     /locus_tag="VGB88_06420"
     CDS             complement(2451..>2894)
                     /locus_tag="VGB88_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9647113.1"
                     /translation="EDIRYYLDMRFRQEPNPLVFVLGDLNDGPGKELLERQFLFFDLV
                     STLQGDVFFARRFLNHALFDYRDDLRWTVAFRDPLDPGRDPHILLDHILFTQGLVDGT
                     RPVEVRPGAGLVEHQIHDRVNAPLTAKQKTSDHKPVSCRITVTDG"
BASE COUNT          433 a         1047 c         1003 g          411 t
ORIGIN      
        1 gcatcggcgg cgcggtgctc gacgtctggt acagctatcc caaggcggac gggccggacg
       61 tggcgccgtc gcgccagccg ttccacgagc tcgacaacgt catcatgacc ccccacgcct
      121 ccggctggac cgagggcctg atcgagcgcc gctggaccgt gatcgccgac aacatagggc
      181 gcctcgccga cgggcggcca ctgcacaacc ggatcgcgcg cccttgaccg ccgccgccgg
      241 cggaccgaaa agggccgcag tgacccgata gcctgggggg cgacgatgcg cagcggacgg
      301 ctgttgacgg cgggcctgat gatcgcggcg acgccggcat gggcacttga cgagatcgtc
      361 accccgcagg tgttcgagat cgccgattac gccaccgccc gcggcgagct aatcgagggc
      421 ctgcgcgtcg gctgggaggc ctatggcaag ctcaatctcg acaaggacaa cgcgatcctg
      481 atcgtgccct acttctcggg cacgccgcac gccgccggca agtacgacgc ggccgacgcg
      541 cagcccggct actgggacgc gatcatcggc cccggcaagc cgctcgacac cggcacctat
      601 tacatcgtca gcgccgatgc gctgtgcgac gtccaggtca aggacccgca cgtcaccacc
      661 accgggccgg cgacgatcga cccgcgcacc ggcgaacgct atggcctcga cttcccggcg
      721 gtctcgatcc gcgactttgt cgcggtgcag aaggcgctgc tggagaacct tggcatcttc
      781 aagctgcgtg ccgtggtcgg cttctcgatg ggcgccgccc aggcgctgca atgggcagtg
      841 gcctacccgc acatggtcga gcgcgcgatc ctggtcacgc cgtgggcgcg cgccgacgcc
      901 tggcagatcg ccaccatata cgcctggacg gcgccgatcc tgcgcgatcc ggcgtggaac
      961 ggcggcgcct attacggtgc gccggagccg gtcgagggcc tggccgcggc ctacgaggtg
     1021 gcgttcatgg acgcgctcgg cccggccttc ctcaacgcca cgttcgggcg caagtgggcc
     1081 gaggacggca acgacccggc ggcggcgccg gacaaccggt tcgcggtcga gacctggctc
     1141 caggataccg cgcgcgccta tgccgagacc ggcgacgcca accacttcct gtgccaggcc
     1201 aaggcgatcc agctgttcga ggccggccag cggggcgaca tcgccgaggc gctggcgcgg
     1261 gtcacggcga agacattgtt gctgccggcg gccggcgaca tcatcctgcc gcatgacctg
     1321 tccgacgaga tcgcggctca actcaccggc gcgcccgccg tcgagcgcgc cgtgattccc
     1381 ggcggcgccg gccacatgaa cggcagcacc gcgatcggcg aggtcggcgc ggcgatcgcc
     1441 cgcttcctcg ccgagtaagc ctgagcctca gcgcaggccg agcgcggcct cgcggccctt
     1501 gaccagcgcg atgcaggtgt cgttgaccgg ggtcgcgacg ccgacctcgc ggccggtgcg
     1561 cgcgacgccg ccgttgatcg cgtcgatctc gctccgcctg ccggcctcga tgtcctggag
     1621 catcgacggc cgtccgtccg ggatcttgag cgcgaaggcg cgcacatacg ccaccgggtc
     1681 gtcgaagtcg aggcggatgc ccttggcgcg cgcgaccgcg tagccctcgc cggcgagccc
     1741 cgccgagacc gcgaaagtat ccgcgtttgc caccaccgcg ccgaccttga gcccggtcaa
     1801 ggtgcacacc gggctgaacg agatgttgca gatcagcttc tcccagatca actggtggat
     1861 gtcgtcgaac gccttgacct tgaagccgcc cgcgcgccag gcctcggcga tgcgctcgag
     1921 gcggggcgtg accgggccgt cgatctcgcc gaggcggatc agctcccagc cctcgtggcg
     1981 ggcgtgcccg gggccgatga tcgagccgcc gaagccgccg gcgacgccga tcaccacccg
     2041 atccggcccg accactccct gcaccgcctc gccgctgccg aggccgttct ggatggccag
     2101 caccggcgtg tcggggccga gcagcgcctt ggcggtcttg gcggcggcgg cgacgtccat
     2161 cgccttggtg gcgatcacca ccagctcgca cgggccgacc tcggcggcgt cggtggtcgc
     2221 gtgcaccgcg accgtgtggt cgccgctcgc gccctcgacg cgcaagccgt gggcgcggat
     2281 cgcctcgata tgctcgcgcc agcggtcgat cgcccacacc tcatggccgc cgcgcgcgag
     2341 cagcgccgca tagaccgaac ccatcgcgcc gacgcccacc accccgatct tcatctccgg
     2401 ttgcccccca gtcccgaatg cgtcgattgt agccgcggcc gtgcccggtt ctagccgtcg
     2461 gtcacggtga tccggcacga caccggcttg tggtcgctgg tcttctgctt agccgtcaac
     2521 ggcgcgttga cgcggtcgtg gatttggtgc tcgaccaggc ccgcgcccgg gcgcacctcg
     2581 accggccggg tgccgtcgac caggccctgg gtgaacagga tgtggtcgag caggatgtgc
     2641 gggtcgcggc cggggtcgag cgggtccctg aaggccacgg tccagcgcag gtcgtcgcga
     2701 tagtcgaaca gcgcgtgatt gaggaaccgg cgggcgaaga acacgtcgcc ctgcagggtc
     2761 gagaccaggt cgaagaacag gaactggcgc tcgagcagct ccttgcccgg gccgtcgttg
     2821 aggtcgccga gcacgaacac cagcgggttc ggctcctggc ggaagcgcat gtccaggtag
     2881 tagcggatgt cctc
//
LOCUS       DASWHU010000172         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1564451, whole genome shotgun sequence.
ACCESSION   DASWHU010000172 DASWHU010000000
VERSION     DASWHU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564451"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGB88_06425"
     CDS             <1..639
                     /locus_tag="VGB88_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647114.1"
                     /translation="VLVDDDEVRAAMTASWLIQMGRREVHVLAGGIGADGLEPGPEPR
                     TVLGLTPARQAFEISVGRMMGMLPGRDMTLIDFADSRSFKAGHVAGAWWTVRARLAET
                     IARMPQSQMVVATSPNSDLAHLAAPEIARHVRCPVRVLGGGTDAWVAAGLPLEAGDAR
                     MLGDQDDVHLLPYDHPPAEIEAAMRAYLNWETALVPRTARDGVARFDVRPRA"
     gene            636..1595
                     /locus_tag="VGB88_06430"
     CDS             636..1595
                     /locus_tag="VGB88_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647115.1"
                     /translation="MSSRSRVIRDSTGREIALAGAPRHVLPTGNPAAAALFCLARDRL
                     QGWPEPVPGEAPPAAPALPSVPRLNAHDAAASATVVARLAPDLVLDFGTCAPPFVAFA
                     ERLQADTGIPVAIVDGRLRETPRSLRLLGRLVGAEARASALAQAFESMWSEAAAAGRG
                     RGPRVHYAIGPRGEKTARRGSIHLDLPQMLGAVNVAEVEAGDGGRVPVAAADVARWAP
                     ELILTIDADFHRDAARLPVWAEMAAVRAGRVYLAPAPVLSWLDYPPSVNRAIGLAWLA
                     ELFHGVIGRDRLAALACDFHRMFYGVDLAPATLAAALSKAGVG"
     gene            1630..2553
                     /locus_tag="VGB88_06435"
     CDS             1630..2553
                     /locus_tag="VGB88_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX9647116.1"
                     /translation="MPILIAAATARAELGSHGHVVNAVAFAPDGRIALSASWDNTVAL
                     WDLATGAELRELDGHGQSVNALAVSPDGTRFASASWDRTVRLWDLASGAPLEVLSGHS
                     EAVYGVAYSPDGSRLVTASWDYTLKLWDLASGRAVRDFIGHEANVMAAQFSPDGARIL
                     SASYDRTLRLWNTGTGAELKVLRGHANSVNDVAVLPDGQTALSASTDRSLRLWDLAAG
                     TEIARLDGHGAFVTCVAVSPDGGQALSGDGDGALILWDLAARRPRRLLTGSEGAVWDV
                     AISPDGARALSGGADHKVRYWDLASGTEIAF"
BASE COUNT          321 a         1007 c          986 g          392 t
ORIGIN      
        1 gtcctggtcg acgacgacga ggtgcgcgcc gccatgaccg cgtcgtggct gatccagatg
       61 ggccggcgcg aggtccacgt gctcgccggc gggatcggcg cggacgggct cgagcccggc
      121 cccgagccgc gcaccgtgct cggcctgacc cctgcccggc aggcgttcga gatcagcgtc
      181 gggcggatga tgggcatgct gcccgggcgc gacatgacct tgatcgactt cgccgacagt
      241 cggagcttca aggccggcca cgtcgccggc gcctggtgga cggtgcgcgc gcgcctggcc
      301 gagaccatcg cgcgcatgcc gcagtcgcag atggtggtcg cgacctcgcc caactcggac
      361 ctcgcgcacc tcgccgcgcc ggagatcgca cgccatgtgc gctgcccggt cagggtgctc
      421 ggcggcggca ccgacgcctg ggtcgcggcg ggcctgccgc tcgaggcggg cgatgcgcgc
      481 atgttgggcg accaggacga tgtccacctg ctgccctacg accatccgcc cgccgagatc
      541 gaggcggcga tgcgcgccta cctgaactgg gagacggcgc tggtgccgcg caccgcgcgc
      601 gacggcgtcg cccgcttcga cgtgcgcccg cgcgcttgag ctcccgttcc cgcgtcatcc
      661 gcgattcgac ggggcgcgag atcgctctcg cgggcgcgcc gcggcacgtc ctgccgaccg
      721 gcaacccggc ggcggcggcg ctgttctgcc tcgcgcgcga ccggctgcag ggctggcccg
      781 agccggtgcc cggcgaggcg ccgccggccg cgcctgccct gccctcggtg ccgcgcctga
      841 acgcccacga cgccgcggcc tccgccaccg tcgtcgcgcg cctggcgccg gacctggtgc
      901 tcgacttcgg cacctgcgcg ccgccgttcg tcgccttcgc cgagcgcctc caggccgaca
      961 ccgggattcc ggtcgcgatc gtcgacgggc ggctgcgcga gacgccgcgc tcgctgcgcc
     1021 tgctcggcag gctggtgggc gcggaggcgc gcgccagcgc gctggcgcaa gcgttcgagt
     1081 cgatgtggtc ggaggccgca gccgctggcc gtggccgcgg cccgcgcgtc cactacgcca
     1141 tcggcccgcg cggcgagaag accgcgcgcc gcggctcgat ccacctcgac ctcccgcaga
     1201 tgctcggcgc ggtcaatgtc gccgaggtcg aggccggcga cggcgggcgc gtgccggtcg
     1261 cggcagcgga cgtggcgcgc tgggcgcccg agctgatctt gaccatcgac gccgacttcc
     1321 accgcgacgc cgcacgcctg ccggtgtggg ccgagatggc ggcggtgcgc gccggccggg
     1381 tatatctcgc cccggcaccg gtgctgtcgt ggctcgacta cccgccctcg gtcaaccgcg
     1441 ccatcggcct cgcctggctc gccgagctgt tccacggcgt gatcggccgc gaccggctgg
     1501 cggcgttggc atgcgacttt caccgcatgt tctacggcgt cgatctcgcc ccggcaacgc
     1561 tcgccgccgc gctttccaag gccggggtgg gctgacagac tcgcgcccat gcgcgccttt
     1621 gccttggcct tgcccattct catcgccgcc gcgacggcgc gggcggaact cggcagccac
     1681 ggccacgtgg tcaacgcggt cgcgttcgcg cccgacgggc ggattgcgct gtcggcgagc
     1741 tgggacaaca cggtcgcgtt gtgggacctc gccaccggcg ccgagctgcg cgaactcgac
     1801 ggccacggcc agagcgtcaa tgcgcttgcc gtttcgcccg acggcacccg cttcgcctcg
     1861 gcgagctggg accgcaccgt acgcctgtgg gacctcgcca gcggcgcgcc gctcgaggtg
     1921 ctcagcggcc actccgaggc ggtctacggc gtcgcctact cgcccgacgg ctcgcgcctg
     1981 gtcacggcga gctgggacta cacgctcaag ctctgggacc tggcgagcgg gcgcgcggtg
     2041 cgcgatttca ttggccacga ggcgaacgtg atggccgccc agttctcgcc cgacggcgcg
     2101 cgcatactgt cggcgagcta cgaccggacc ttgcgcctgt ggaataccgg gaccggcgcg
     2161 gagctcaagg tcctgcgcgg ccacgccaac agcgtcaacg acgtggccgt cctgccggac
     2221 gggcagacgg cgctgtcggc aagcaccgac aggagcttgc ggctctggga cctcgccgcg
     2281 ggcaccgaga tcgcgcgcct cgacggccac ggcgcgttcg tcacctgcgt cgcggtctcg
     2341 cccgacggcg gccaggcgct gtctggcgac ggcgacggcg cgctgatcct atgggacctc
     2401 gccgcgcgcc ggccacggcg cctgctcacg ggctccgagg gcgcggtctg ggacgtcgcc
     2461 atctcgccgg acggcgcgcg cgccctgtcc ggcggcgccg accacaaggt tcgctactgg
     2521 gaccttgcct cgggcaccga gatcgcgttt tgaagctgtt ggcacctagt acatcgtttc
     2581 catggatcga atcatatcaa gcggcgcggt cgagcttgta ggtccaggtg tggacgacgg
     2641 ggtggcgatt gatgtggtcg atggcggtca tgaggcggtc cttgagttcc tgctcggatg
     2701 cgacac
//
LOCUS       DASWHU010000173         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1184210, whole genome shotgun sequence.
ACCESSION   DASWHU010000173 DASWHU010000000
VERSION     DASWHU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184210"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGB88_06440"
     CDS             complement(<1..248)
                     /locus_tag="VGB88_06440"
                     /inference="COORDINATES: protein motif:HMM:NF045145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647117.1"
                     /translation="MAGSCSPAPAPSCWRTRRCAAPISKAATRVEAVEALSETSLGVF
                     IGVTVVLGGGAGILTGRAVAATWRPVSQLLFACVGLGLA"
     gene            complement(157..873)
                     /locus_tag="VGB88_06445"
     CDS             complement(157..873)
                     /locus_tag="VGB88_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647118.1"
                     /translation="MLSVESVHTFYGPIQALRGVDLEVGEGEIVTLIGANGAGKSTLL
                     MTVCGTPQARAGRIVFRGQDITRVPTFRIVRLGIAHAPEGRRIFSRMTIMENLQMGAV
                     TADPAEFGRDLERVFALFPILGDRRHQRGGTMSGGEQQMLAIARALMSRPRLLLLDEP
                     SLGLAPMLVKQIFEVIGEINRSQGVTILLVEQNAHHALRVAHRGYVMANGRIVLAGTG
                     AELLANEEVRGAYLEGGHQG"
     gene            complement(873..1691)
                     /locus_tag="VGB88_06450"
     CDS             complement(873..1691)
                     /locus_tag="VGB88_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9647119.1"
                     /translation="MTGRALLEVEHLTMRFGGLVAVDDLSFRAAEREITAIIGPNGAG
                     KTTVFNCLTGFYQPTVGRLALRGHDGRESFLERMEGFRIARAGIARTFQNIRLFPAMS
                     VLENLIVAQHQPLMRASMFSVAGLLGLARYRDAEAEAVAHACTWLERVGLADDADRVA
                     GELPYGAQRRLEIARAMCTSPVLLCLDEPAAGLNPRESAELNRLLLEIRDEHGIGLLL
                     IEHDMSVVMGISDHIVVLDYGRKIADGTPARIRADPAVIRAYLGETEDVEPGAA"
     gene            complement(1688..2938)
                     /gene="livM"
                     /locus_tag="VGB88_06455"
     CDS             complement(1688..2938)
                     /gene="livM"
                     /locus_tag="VGB88_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEX9647120.1"
                     /translation="MVKEAGLAALVAAVLGLPLIGLRVMPGPNDLEVVARWPWLAIAV
                     AAVFCGRIAVILLGRARPAFANLGGSVAAEIGGLVRRRRMPLAAVALLLAVTLPLMPF
                     ADRYVVDVATLVLIYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYSFALLGTTYDLSF
                     WVCLPIAGAIAASFGILLGYPVLRLRGDYLAIVTLGFGEIIRIILTNWVAFSNGPNGI
                     PGIPRPSLFGLEFTRSSATGTTFHEFFGLEFSGDQRITYLYFIILALALLTNAFVLRI
                     RRLPIGRAWEALREDEIACRALGLNPVNIKLSAFALGAMFGGFSGSFFAARSGFISPE
                     SFTFIESAIILSIVVLGGMGSQIGVVFAAGAIILLPEALREFSQFRMLVFGIAMVLIM
                     IWRPRGLLAHREPTIRLARAGVRA"
     gene            complement(2991..3905)
                     /locus_tag="VGB88_06460"
     CDS             complement(2991..3905)
                     /locus_tag="VGB88_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389504.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEX9647121.1"
                     /translation="MAYFLQQLINGLTLGSIYGLIAIGYTMVYGIIGMINFAHGEIYM
                     IGAFIAVISFTILGAAGITWVPLALLVVLVVAMLFTSVYGWTVERVAYRPLRGSQRLA
                     ALISAIGMSLALQNYVLITQGAGVKALQPIIAGGFTVYQGGDFRVTLSYIQIFIMVLT
                     VVLMGAFATLIAQSALGRAQRACEQDRTMATLLGIDVDRTISLTFVVGAALAAVAGVM
                     VSLYYGVVDFFMGFIAGLKAFTAAVLGGIGSLPGAMLGGLLLGMVEAFWSGYFTVEYK
                     DVAAFGILVLVLIFRPTGLLGRPEIEKV"
     gene            complement(4037..4249)
                     /locus_tag="VGB88_06465"
     CDS             complement(4037..4249)
                     /locus_tag="VGB88_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647122.1"
                     /translation="MASGEEPDGDGIIYEFIRIGGSVKVTAVDPVTYIEVSIVGSPLV
                     SDAELMRLARRKLDYVLAKRGRPRAR"
     gene            complement(4283..>4915)
                     /gene="hemA"
                     /locus_tag="VGB88_06470"
     CDS             complement(4283..>4915)
                     /gene="hemA"
                     /locus_tag="VGB88_06470"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX9647123.1"
                     /translation="PIEEICDVAQRHGALAYLDEVHAVGMYGPRGGGVAEREGLMDRL
                     DIIEGTLAKAFGVMGGYIAGSANLIDVVRSYAAGFIFTTSVPPAVAAGALAAVRHLKQ
                     SQAERERHQERARTLKRRLAEIGIPVLDNPSHIVPVMIGDAVLCKRASDELLRDFAIY
                     VQPINFPTVERGTERLRITPSPLHTDEMMDHLVEALRSVWARLGINRAAA"
BASE COUNT          835 a         1695 c         1754 g          631 t
ORIGIN      
        1 gcgaggccga gaccgacgca ggcaaacaag agctgcgaca ccggccgcca ggtggccgcg
       61 accgcgcgcc cggtgagaat cccggcgccg ccgccgagca ccacggtgac gccgatgaac
      121 acgccgagcg aagtttccga cagcgcttcc accgcctcaa ccctggtggc cgccttcgag
      181 ataggcgccg cgcacctcct cgttcgccag cagctcggcg ccggtgccgg cgagcacgat
      241 ccggccattg gccatgacgt agccgcgatg ggcgacgcgc agcgcgtggt gcgcgttctg
      301 ctcgaccagc aggatggtca cgccctggct gcggttgatc tcgccgatca cctcgaagat
      361 ctgcttgacc agcatcggcg cgagcccgag cgacggctcg tcgagcagca atagccgcgg
      421 ccggctcatc agcgcgcggg cgatcgcaag catctgctgc tcgccgcccg acatcgttcc
      481 accgcgctgg tggcggcggt cgccgaggat cgggaacagc gcgaacaccc gctcgagatc
      541 gcgcccgaac tcggccgggt ccgcggtcac cgcgcccatc tgcaggtttt ccatgatcgt
      601 catgcgcgag aagatgcgcc ggccctccgg cgcgtgcgcg atgccgaggc ggacgatgcg
      661 gaaggtgggg acgcgggtga tgtcctggcc acggaagacg atccggcccg cgcgcgcctg
      721 gggcgtgccg cagacggtca tcagcagggt cgacttgccg gcgccgttgg cgccgatcag
      781 ggtgacgatc tcgccctcgc cgacctcgag gtcgaccccg cgcaaagcct ggatgggccc
      841 gtagaacgtg tgcacgctct cgaccgacag catcatgcgg cgcccggctc cacgtcctcg
      901 gtctcgccaa gataggcgcg gatcacggcc gggtccgcgc ggatccgggc cggggtgccg
      961 tcggcgatct tgcgcccgta gtcgagcacc acgatgtggt cggagatgcc catcaccacg
     1021 ctcatgtcgt gctcgatcag cagcaggccg atgccgtgct cgtcgcggat ctcgagcagc
     1081 agccggttga gctcggccga ttcgcgcggg ttgagcccgg ccgcgggctc atcgaggcac
     1141 agcagcaccg ggctcgtgca catggcgcgc gcgatctcca ggcggcgctg ggcgccgtag
     1201 ggcagctcgc ccgcgacccg gtcggcgtcg tcggccagcc cgacgcgctc gagccaggtg
     1261 caggcgtgcg ccaccgcctc ggcctccgcg tcgcggtagc gggcgagccc gagcaggccg
     1321 gcgaccgaga acattgaggc gcgcatcagc ggctggtgct gggcgacgat caggttctcg
     1381 agcaccgaca tggccgggaa caggcggatg ttctggaagg tgcgcgcgat gccggcgcgg
     1441 gcgatgcgga acccctccat ccgctccaga aagctctcgc gcccgtcatg gccgcgcaag
     1501 gcgaggcggc cgacggtcgg ctggtagaac ccggtcaggc agttgaacac cgtggtcttg
     1561 ccggcgccgt tggggccgat gatggcggtg atctcgcgct ccgcggcgcg gaacgacagg
     1621 tcgtcgaccg cgaccaggcc gccgaagcgc atggtcaggt gctcgacctc gagcagcgcg
     1681 cgcccggtca tgcgcgaacg ccggcgcgcg ccaggcggat ggtcggctcg cgatgggcga
     1741 gcagcccgcg cggccgccag atcatgatca gcaccatcgc gatgccgaac accagcatgc
     1801 ggaactgcga gaactcgcgc agcgcctcgg gcaacaggat gatcgcgccg gccgcgaaca
     1861 ccaccccgat ctggctgccc atgccgccga ggacgacgat cgacaggatg atcgcggact
     1921 cgatgaaggt gaagctctcc gggctgatga agcccgagcg ggcggcgaag aacgagcccg
     1981 agaagccgcc gaacatcgcg cccagcgcga acgccgacag cttgatgttg accgggttga
     2041 ggccgagcgc gcggcacgcg atctcgtcct cgcgcagcgc ttcccaggcg cgcccgatcg
     2101 gcaggcggcg gatgcgcagc acgaaggcgt tggtgagcag cgccagggcg aggatgatga
     2161 agtagagata ggtgatccgc tggtcgcccg agaactcgag gccgaagaac tcgtggaagg
     2221 tggtgccggt cgcggagctg cgcgtgaact cgagcccgaa cagcgacggc cgcgggatgc
     2281 cggggatgcc gttcgggccg ttcgagaacg cgacccagtt ggtcaggatg atgcgaatga
     2341 tctcgccgaa gccgagggtg acgatggcga gataatcgcc gcgcagccgc agcaccggat
     2401 agcccagcaa gattccgaac gaggccgcga tcgcgccggc aatgggcagg cagacccaga
     2461 acgacaggtc gtaggtggtg ccgagcaggg cgaagctgta ggcgccgacg gcgtagaacg
     2521 cgacgtagcc gaggtcgagc aggccggcca ggccgacgac gatgttgagc ccccagccga
     2581 gcatgacgta gatcagcacc agggtcgcca cgtcgaccac gtaacggtcg gcgaaaggca
     2641 tcagcggcag cgtcaccgcg agcagcaggg cgaccgccgc gagcggcatc cgccggcgcc
     2701 ggacgaggcc gccgatctca gccgcgaccg agccgccgag gttcgcgaac gccggtcgcg
     2761 cgcgcccgag caggatcacg gcgatgcggc cgcagaacac cgccgccacc gcgatcgcga
     2821 gccacggcca gcgcgcgacg acctcgaggt cgttcggccc cggcatcacc cgcagcccga
     2881 tcaggggcag gccgaggacc gcggcgacca gcgccgcgag gcccgcctcc ttgaccatcg
     2941 ccgaggccgc ggggaggcgc gcggccggca cggccacctc ttgcacccgc tcagaccttc
     3001 tcgatctcgg gccggccgag cagcccggtc ggccggaaga tcagcaccag caccaggatg
     3061 ccgaacgcgg cgacgtcctt gtactcgacc gtgaagtagc cggaccagaa cgcctcgacc
     3121 atgcccagca gcaggccgcc gagcatggct ccgggcagcg agccgatgcc gccgagcacc
     3181 gccgcggtga acgccttcag cccggcgatg aagcccatga agaagtcgac cacgccgtaa
     3241 tagagcgaca ccatgacgcc cgcgaccgcg gccagcgccg cgccgaccac gaaggtgagc
     3301 gagatggtgc ggtcgacgtc gatgccgagc agggtggcca tggtccggtc ctgctcgcag
     3361 gcgcgctggg cgcgcccgag cgcggattgg gcgatcaggg tcgcgaacgc gcccatcagc
     3421 accaccgtaa gcaccatgat gaagatctgg atatagctga gcgtgacccg gaagtcgccg
     3481 ccctgataga cggtgaagcc gccggcaatg atcggctgca gcgccttgac gcccgcgccc
     3541 tgggtgatca gcacgtagtt ctgcagcgcg agcgacatgc cgattgccga gatcagcgcc
     3601 gccaggcgct gcgagccgcg cagcggccgg taggcgacgc gctcgaccgt ccagccatag
     3661 accgaggtga acagcatcgc caccaccagc accacgagca gcgccagcgg cacccaggtg
     3721 atgccggcgg cgccgaggat ggtgaagctg atgaccgcga tgaaggcgcc gatcatgtag
     3781 atctcgccat gggcgaagtt gatcatgccg atgatgccgt agaccatggt gtagccgatc
     3841 gcgatcagcc cgtagatgct gccgagcgtg agcccgttga tcagctgctg aaggaagtac
     3901 gccatgcggt cccctgcccc cacccgcggc ccccggcatc ggccggcgat ggcggcgccg
     3961 gccgatcacg cggcccctgt ctagcgcagt tgaggcggcg gcgaaaggcg gctctgttcg
     4021 cgatcagggg gcagggtcag cgcgcccgcg gccggccgcg cttggcgagc acgtagtcga
     4081 gcttgcgccg cgcgagccgc atcagctcgg cgtcgctgac cagcggcgag ccgacgatcg
     4141 agacctcgat gtaggtgacc ggatcgaccg cggtcacctt gaccgagccg ccgatgcgga
     4201 tgaactcgta gatgatgccg tcaccgtcgg gctcttcgcc gctcgccatg gcgctggtgc
     4261 gggcccggca gggcgggcgc ggtcaggccg cggcgcgatt gattccgagc ctggcccaga
     4321 cgctgcgcag cgcctcgacc aggtggtcca tcatctcgtc ggtgtgcagc ggcgagggcg
     4381 tgatcctgag ccgctcggtg ccccgctcca cggtcgggaa gttgatcggc tggacgtaga
     4441 tggcgaagtc gcgcaacagc tcgtcgctcg cccgcttgca cagcaccgcg tcgccgatca
     4501 tcaccggcac gatgtggctc gggttgtcga gcacgggaat ccctatctcg gcgaggcgcc
     4561 gcttgagtgt gcgcgcgcgc tcctggtggc gctcgcgctc ggcctggctc tgtttgaggt
     4621 ggcgcaccgc ggcgagcgcg cccgccgcca ccgccggcgg caccgaggtg gtgaagatga
     4681 agcccgcggc ataggagcgc acgacgtcga tcaggttggc actgcccgca atgtagccgc
     4741 ccataacgcc gaacgccttg gcgagcgtgc cctcgatgat gtcgaggcgg tccatcaggc
     4801 cctcgcgctc ggcgacgccg ccgccgcgcg gcccatacat gccgaccgca tgcacctcgt
     4861 cgagataggc gagcgcgccg tggcgctggg cgacgtcgca aatctcctcg atcgg
//
LOCUS       DASWHU010000174        18460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1566023, whole genome shotgun sequence.
ACCESSION   DASWHU010000174 DASWHU010000000
VERSION     DASWHU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18460
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566023"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..1096
                     /locus_tag="VGB88_06475"
     CDS             623..1096
                     /locus_tag="VGB88_06475"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9647124.1"
                     /translation="MARTKTAKPARAKRATKAAKPRKAARAKPARAMTAARKAAPKAK
                     ARRPVARRAAPRAAVRVGATPMGKSDKDHLAAVIQGSTGCTAKIAKDALDNVISTITE
                     SLKKKGKVQLTGFGSFAVTRRAARMGRNPRTGEKVKIKASRSVRFRAGKTLKDAV"
     gene            1258..1677
                     /locus_tag="VGB88_06480"
     CDS             1258..1677
                     /locus_tag="VGB88_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX9647125.1"
                     /translation="MKQSLHRLTVATRGQGLVEITETVSAWLATQAIRQGLLTLFVRH
                     TSASLLIQENADPDVLRDLESFFASLAPEDATLYRHRTEGPDDMPAHIKSALTQTQIS
                     VPVDEGRLLLGTWQGVYLFEHRTAPHRREVVLHLAGI"
     gene            1710..2549
                     /locus_tag="VGB88_06485"
     CDS             1710..2549
                     /locus_tag="VGB88_06485"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9647126.1"
                     /translation="MAFEQITYGVDAHIATVTLNRPDKLNAWTPVMEREVRRAMAAAA
                     ADAAVRVIVLTGSGRGFCAGADMSVLSGLAGTTAAERAEAARRIAAAAPIPGALELGD
                     DFSGRLAYFPTIPKPVIAAINGPTAGLGFVLTLFADLRLAAADAFLTTAFSRRGLIAE
                     HGIDWMLPRLVGLGAAFDLLLSARRVGAEEALAMGLVNAVYAADGFRDQVRAYAGELA
                     TAVSPRSMRVMKRQLYLSGARGLGEAVRSGDQEMLASFDSEDFREGVAHFRDGRAPAF
                     TGR"
     gene            2677..3669
                     /locus_tag="VGB88_06490"
     CDS             2677..3669
                     /locus_tag="VGB88_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647127.1"
                     /translation="MFSKPAVPKSDVAPRHESLAVPRRPARRDTPMVAPIDLALLAGL
                     PVLTALSWLLPPAAWPAVARALTPFQLREMARDPDALARVVARICGPRSGEVDGRDVV
                     ERLARHDLMALLLMLRALRPGAAPPAIRLSGGERIAAALADGRGAILWIGYFAYASMV
                     AKMALHEAGHRIVHLSHPRHMFSSSWLGMRVINGVARNAEDRFLGERVLLGLDSATAA
                     LRTLAHHLGENRVVSVAARSTATRPVRVGFLGRELALAPGALDLSFRTGAPVLPVFTV
                     RAHDGFEVVVEPALTVDRSLPRRRATREAAQAFARLLESWVLSHPDQWQDWQHL"
     gene            complement(3672..4637)
                     /locus_tag="VGB88_06495"
     CDS             complement(3672..4637)
                     /locus_tag="VGB88_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9647128.1"
                     /translation="MLTRLGLVAGIALMFALVVWRGIEPVAQALVALGWGLLLLPPAY
                     LPSLGLAAASWRPLFRPRREPSLAAALRATWIGNSVSLLLPVTGLGGEAVRARLVALD
                     GIRARDAAASVIAGQAVQALSLAAWALLGAGLLAARTGESALAIAVTIAAGLVVAGTL
                     GFVAVQHAGVAGRVAGAFAGAVRGGDGMTAKSGGLRLDAALRRIYARPRRLALALALH
                     LAARVALALEVWLAAYLIGMSLTFTDALMVKSLTGAARGLLFMVPGGIGIQEAGYVAL
                     AAVVDASPAALLAVSLATRGRELLIGAPGLLAWHRAEARALRGRA"
     gene            complement(4799..5173)
                     /locus_tag="VGB88_06500"
     CDS             complement(4799..5173)
                     /locus_tag="VGB88_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9647129.1"
                     /translation="MAGHGTFGWNELITPDTDGCGAFYGKLLGWTRRDMDMGTGNYTL
                     FNNGAQDVGGMMKPPADGAGPSMWLAYVMVDDVDARTAKVEGLGGKVMKAPFDIPGVG
                     RISIVTDPSGAPIGLFTAAAEA"
     gene            complement(5211..5633)
                     /locus_tag="VGB88_06505"
     CDS             complement(5211..5633)
                     /locus_tag="VGB88_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX9647130.1"
                     /translation="MTLVEWKPEFSLGIPAVDHEHRELIALINELHAGLETAAGRDSV
                     IAFFGEIYARISAHFALEEAIMRERAYDQYGEHKAQHEDLLDQIRDIMDDFEAGAYDD
                     YQDRLSSALAAWFTDHFKTHDSRLHRMLGDAHPWIGEI"
     gene            complement(5639..6172)
                     /locus_tag="VGB88_06510"
     CDS             complement(5639..6172)
                     /locus_tag="VGB88_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647131.1"
                     /translation="MARLGAYLGQGVVYALVALVLGTFSNTPAYRGFPADSAELVLSF
                     SHGAARKGDCRRLTPEEIAALAPNMRRPEICPRERVPVTVRLALDEQVMFEHALPPGG
                     IAGDGPSRVYRRFTVPAGRHLIAAALRDTERESGFDYAAEQTVTLAAGARFVVDFKAE
                     LGGFTFDSRPVAAERAR"
     gene            complement(6165..7706)
                     /locus_tag="VGB88_06515"
     CDS             complement(6165..7706)
                     /locus_tag="VGB88_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9647132.1"
                     /translation="MTAPIRIARSGLERAEALLDRIFGARWNPLYQLGALGFLFYWVI
                     AVSGIYVYVLFGTGTTEAYRSVEYLTIDQWWLGGVMRSLHRYASDAMVLVLVVHAARE
                     FLLGRLRGPRWFSWVTGTPILWLVFISGISGYWLVWDRLAQYIAIRSTEWFDWLGIFG
                     EPIARNFLTPAALDDRFFTLLMFIHIVVPLLLLLVLWLHLQRISRPRITPNRGLLVGT
                     LAMLLVLSLVKPAVSHAPADLAQVPSVLRLDWFYLVFYPLIDHWSAGAVWGFAAALTV
                     ILVALPWLPPRRAAPAAVVHLDNCNGCGRCAADCPYAAILMRPRSDGLPFRDQAHVDP
                     ARCVACGICAGACPTATPFRRKSELRPGIELPDRSVADLRETVHAAAATVTGSPRVLV
                     IACDHCAGAGGAGGATVAVPCIAAVPPPFIDYVLSRDLADGIVLAGCRAGNCYHRGGI
                     DWTEARIAGTRDPYLRRRVPRGRLLTVWAAQSEGARLAAAIASFRDRIQPRPRCRAEP
                     IGEHG"
     gene            complement(7721..8548)
                     /locus_tag="VGB88_06520"
     CDS             complement(7721..8548)
                     /locus_tag="VGB88_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9647133.1"
                     /translation="MRWLRWLSVPTAVVLAAAGAANAAAKVAPEPSAFDPDAAIAYSQ
                     AAIGREVGDLAFTGSDGTPVRLAGFRGKPLVVNMVFTGCALSCPVVVQRLYRAVEIAQ
                     DAVGSDRFSVITVGFDTDADTPGRMRDFARAQGLDLPNWEFLSGDEASVRALADVLGF
                     IYFPSPRGFDHLAQTTVVDAEGRVFRQVYGAEFEPPAVVEPLLALVYGGGKGIASLAD
                     AVDRLRLFCTYFDPTTGRYRFDYSIFIALAVGVASLGGIAFVLVRAWLRAAPPAPHG"
     gene            complement(8559..9425)
                     /gene="cyoE"
                     /locus_tag="VGB88_06525"
     CDS             complement(8559..9425)
                     /gene="cyoE"
                     /locus_tag="VGB88_06525"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9647134.1"
                     /translation="MATLVGQVWTVFKLRIAFAIMLCALAGMAVAGAPALPAWKASVL
                     ALAVLLSAAAAGAFNHYVERDLDARMARTRTRPFVTGAFRAGPAWLALIFAVLAVAVG
                     AAGWALNGWAALYTFLGAFVYGVVYTVWLKRRTWLNIVLGGLAGSFAVLAGAAAVEPD
                     LGAVPLILAGVLFLWTPPHFWSLATVLHEDYARAGVPMLPVVVGDSAAARIILAHTVA
                     LVALSLVPAALGLGWIYLAGAVVGGAWFLRQSVRLVRAPGAEAARANFGASLVQLGLL
                     LGATLLDRALLG"
     gene            complement(9461..11182)
                     /locus_tag="VGB88_06530"
     CDS             complement(9461..11182)
                     /locus_tag="VGB88_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9647135.1"
                     /translation="MTAEPLEGAVPFREPRYRTCLDTGLKVFAGTETLIRVNAVTAVV
                     FLAVGGLFGVLVALTRWPAVHILPADWFYLALTAHGAAVLLFWIIFFEIAILYFASSI
                     LLNCRLATPAFAWLGYVLMLVGSLMATVAVLQGDSSVMFTSYVPMQAAPHFYLGLILF
                     AVGALIGCFVFFGTLVVAKDEETYEGSIPLVTFGALTAAIIAVFTIASGAIILIPTFL
                     WSVGYITEIDSVTYKLVWWGMGHASQQINVSAHVAVWYAIAAMVLGAKPLSEKVSRTA
                     FLLYILFLQLAAAHHMLAEPGISSEWKVFNTSYALYLAVLGSMIHGMSVPGAVEAAQR
                     RRGYTQGVFGWLRNAPWGNPAFSGMALSLVMFGFLGGISGVVLGTEQINLLMHNTLYV
                     PGHFHGTVVAGTTLAFMAITYLVVPLIFRREIIFKRMAKWQPYVFGIGAAGISLFMMG
                     AGTLGVSRRHWDITFSDAALSWDYPGTAYLMMGLNGLSGLLAGLGGLMFVVIVVGSVL
                     FGPRRDAEAVKANPIIAPGAAVSTYGSEGTLKLPGTLTLVAIFFAAFVLYYFINWKFL
                     SEVWPLS"
     gene            complement(11192..11731)
                     /locus_tag="VGB88_06535"
     CDS             complement(11192..11731)
                     /locus_tag="VGB88_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEX9647136.1"
                     /translation="MAIYPPEKRIWWSEPLDRMEITWISIAFVWGLVMFGAMIWWHIN
                     GRQNLSNEAYRVDPSAFDERTEAMVDQYTVREEGDSGFPVVHPPPGSEIYLRARLWEW
                     YPILELEKDQSYRLHLSSMDWQHGFSLQPANINIQVHPNYEMVLTIEPNEAGEYAVIC
                     NEYCGIGHHTMLGKIYVVE"
     gene            complement(11742..11912)
                     /locus_tag="VGB88_06540"
     CDS             complement(11742..11912)
                     /locus_tag="VGB88_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647137.1"
                     /translation="MTEPTTGESGARRVRVPVMQKVLDNPFLLLFLGVAIPTVFYLMW
                     GVMEVAQIPISK"
     gene            12365..13105
                     /locus_tag="VGB88_06545"
     CDS             12365..13105
                     /locus_tag="VGB88_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9647138.1"
                     /translation="MNRTDLGPEDIQVLRSQPLFRGLDGRALADLLSDARVRTYPRAS
                     LLFLQGEPADRFFVIFAGWVKLFRQSEAGEESVIAVFTAGESFAEAAIFDQATYPVSA
                     VVVEEARMLVVPGASFIRRLSDNGAYALNMMASMSRHLRQLVQQVEQLSVRSSTERVA
                     GFLARLCGEAEAGARVQLPLEKALIAGRLGMQPETFSRALARLRDQGVTSSGGEIVIR
                     DVGSLRRIGGGAVADGRPSRVTHRALRA"
     gene            complement(13080..13757)
                     /locus_tag="VGB88_06550"
     CDS             complement(13080..13757)
                     /locus_tag="VGB88_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9647139.1"
                     /translation="MAEAARIIAAPAGAAESLKDQAYEHIEELIVTLALAPGTTISEA
                     TLAKRLGMSRTPIREALQRLARQGLVRILPRRGLLVTDLDIGKQLRMLEVRREVDRLL
                     VARAAARRTPAQAADFSDSAAAFAEAGDGDDDTLFTRTDRRFNLLLTSAAGNEFAAEA
                     ASLMQGLSRRFWYAHHGRRSSVREAAHLHRDIALAIADGDAERAAAASDALLDHVAAF
                     TRATLGA"
     gene            complement(14115..14912)
                     /locus_tag="VGB88_06555"
     CDS             complement(14115..14912)
                     /locus_tag="VGB88_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9647140.1"
                     /translation="MNVMKRERPPAEIYDEKFVPALFGQWGPVVSNVAGVAPGQAVLD
                     VACGTGALTLAAAAVAGPDGCVVGLDANPEMLAVARRKSADIDWRVGVAENLPFDDAS
                     FDAVISQFGLMFFDDRPRALSEMMRVLKPGGRLAVAVCGALERSPGYAAFATLLDRLF
                     GEEVGNAFRAPFVLGDADDLGRTAAAAGIEGASVEERNGTVRFQTIAELVATERACVW
                     TLGGILDQDQFDLLLGESEEALRPFADNDGIVSFDMPALILMAHKMG"
     gene            14989..16068
                     /locus_tag="VGB88_06560"
     CDS             14989..16068
                     /locus_tag="VGB88_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9647141.1"
                     /translation="MPPAHDQPSETLAGASETHVAARVGRYDPLSDVLRTVKLKGALF
                     FLVDATSPWCVDIPPARDFADIILPRARHVVSYHVIVEGHGLASVPRIDPVHFDAGDI
                     IVFPHADPYLMSSAPGVPPELDRRQTLQFFRDLAAGKLPFVIPEGGGKLPKAKFICGF
                     LGCDLSPFNPIFATLPRILHIRRPSGDHADLLDQLIDLAMVEVGTTRAGGESIRLGLS
                     ELMFVELLRRHLETLSGDAPGWLAGLRDPNVGRALALLHSEPARDWTLVALARAAGVS
                     RSVLAERFARCVGETPMRYLALWRMQLAARLLVDGATKVAVVGEQVGFRSEAAFSRTF
                     KRVTGFSPAEWRRTSANPLARGPGR"
     gene            16281..16622
                     /locus_tag="VGB88_06565"
     CDS             16281..16622
                     /locus_tag="VGB88_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647142.1"
                     /translation="MAGRLRGLAPAIALAAIISAGPVPESAEADSRSSTSINGRTITV
                     VGGSSQSISSTAERTVVKLDGSRIVIRDGRIELEDAVRVVGDFTKLEIRARDGRIDIF
                     VDGEPIDEAIE"
     gene            16765..17409
                     /locus_tag="VGB88_06570"
     CDS             16765..17409
                     /locus_tag="VGB88_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647143.1"
                     /translation="MGRIEVVGRRVELVSMDPHFHDISIALNRLDSDDGCAYLVHTYS
                     GRDGAGARIASVVAAMTTMGDMEAVPGDARLLRFPCRAAHQFACRRLFLEACKIAPGA
                     AADAKPLAVLDRKSAHTICGESLGGGAYSFGLEGGGSGGAERAAVLARGLVKLAELDA
                     ADDQPYRAAFACGQAHDALIGLLMARALNVRAALREVEAQAGRGVLAAPSAQKQ"
     gene            17435..>18460
                     /locus_tag="VGB88_06575"
     CDS             17435..>18460
                     /locus_tag="VGB88_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX9647144.1"
                     /translation="MTDTSQSRPASMTSRERVLAALRRAPVDRTPVCNPTSVATVELM
                     DLVDAPFPDANRDPELMARLAATGHTELGFDSIMPVFSIIQESSALGCKMQWEQKDNW
                     PTVRMREPIFREPDDIVIPNDLLTHPDTACVTEAIRILKREFGDEVAIIGKTMGPWSL
                     GYHCFGVEPFLLMSLDDPGKTKTCLDRLKEATVQFGLAQIEAGADALTLPDHATGDLV
                     SGEYYARYLKDIHIEFAERLPVPLILHICGRTVDRMPFIAETGMAAFHYDSKNKPGES
                     MAAVHGGIALVGNINNPETLFSKGPAEVRAEVFRNLEAGVQLIGPECAIPLQTPLENL
                     IEIPRAVR"
BASE COUNT         2987 a         6462 c         6400 g         2611 t
ORIGIN      
        1 aaaaccaagg aaatataagg catttcccgc atgcctccgg acttccgcac gctcgcgatc
       61 gcccggcgcc gatcaccaag ccggcggccg gccggtcgga cccgtgtcgc gttcggcttc
      121 gctcggccgc agagcggagg ccggaaaacc ggtccggttg cccgcgcatc gtcgccgctc
      181 gttcgccggc taagtggctc gttcggcgcc ggtgcgccgc gcgagtcgaa gcggtttgcg
      241 caaataaatt cgacctgttg cgtgggggtc acgccatgag gcgaaatgcg ttgcgccacg
      301 aagcctctta ggctttgtct tgcaaacaac ctcggctgac tcgaaaatcc gcggatgtcc
      361 atggccgctt ggaaaagcca gtctccggcg gcgctgacaa ggttcgttgc gaatgacgca
      421 tcgcgggtct gccggaaccg acggaagcca tcgcagtgcc gcacgcgcgg cggggatggt
      481 tgaaggttcc ccgcgcccga tgtcgatcgc gtcacaccgg acccggttcc gttcccggtt
      541 cccggcctga gagtgccgac gccgggaacg gcgcaacgtc tcattctcta gcaccgtggc
      601 attaacaact ggagcacgac agatggcgcg taccaagact gcgaaaccgg ccagggcgaa
      661 gcgggccacc aaggctgcca agccgcgtaa ggccgcgcgg gcgaagcccg cccgggccat
      721 gacggcggcg cggaaagcgg cgcccaaggc caaggcgaga cggcctgtgg cccgccgagc
      781 ggcccccagg gcggccgttc gcgtcggcgc gaccccgatg gggaaaagcg acaaggatca
      841 tctcgccgcg gtcatccagg gttcgaccgg ctgcaccgcc aagatcgcca aggacgctct
      901 cgacaacgtg atctcgacga tcaccgagag cctgaagaag aaaggcaagg tgcagttgac
      961 gggcttcggc tcgttcgcgg tgaccaggcg cgccgcgcgc atgggccgca atccgcgcac
     1021 cggcgagaag gtgaagatca aggcctccag gtcggtgcgc ttccgtgccg gcaagaccct
     1081 caaggatgcg gtctgagcac gaccgatctt aatccggtac cggcaccgcc ggccggatat
     1141 tggcgcgaac cgcattcccg atccggccgg ccggctgcac atagtcggcc ggctttcttt
     1201 cgcggcttcg cgcgccgcca tgcccgcgat gtccgcgccg cctgctatcc tggcgagatg
     1261 aaacaaagcc tgcatcgcct gacggtcgcc acccgcggtc agggcctggt cgagatcacc
     1321 gagacggtct cggcgtggct cgcgacccag gcgatccgcc aggggctgct gacgctgttc
     1381 gtccgccaca cctcggcgtc gctgctgatc caggagaacg ccgatcccga cgtgctgcgc
     1441 gatctcgaat cgttcttcgc ctcgctagcg ccggaggatg cgaccctcta ccggcatcgc
     1501 accgaggggc cggacgacat gcccgcccac atcaagagcg cgctcaccca gacccagatt
     1561 tcggtgccgg tggacgaagg gcggctgctg ctcggcacct ggcagggagt ctatctgttc
     1621 gagcaccgca ccgccccgca tcggcgcgag gtggtgctgc atctggccgg catatagcgg
     1681 cgcgcaccgg caaacgcgga gcaccggcca tggcattcga gcagatcacc tatggcgtcg
     1741 atgcgcacat cgccaccgtc accttgaacc ggccggacaa gctcaacgcc tggacgccgg
     1801 tgatggagcg cgaggtgcgc cgggcgatgg cggcggcggc ggcggatgcg gcggtgcgag
     1861 tcatcgtgct gaccggctcg gggcgcggct tctgcgccgg cgccgacatg tccgtgctgt
     1921 cgggcctggc cggcacgacg gcggcggagc gggcggaggc ggcacgacgg atcgcggcag
     1981 cggcgccgat ccccggcgcg ctcgagcttg gtgacgattt ttccgggcgg ctcgcctatt
     2041 tcccgaccat cccaaagccg gtgatcgcgg cgatcaacgg cccgacggcc ggcctcggct
     2101 tcgtgctgac gctgttcgcg gaccttcgcc tcgccgccgc cgacgcgttc ctgaccacgg
     2161 cgttctcgcg ccgcggcctg atcgccgagc acggcatcga ctggatgctg ccgcgcctgg
     2221 tcggcctcgg cgcggcgttc gacctgctgc tgtccgcgcg ccgcgtgggt gccgaggaag
     2281 cgctggcgat gggcctggtg aacgcagtgt atgcggccga cgggttcagg gaccaggtcc
     2341 gcgcctatgc cggagagctt gccaccgcgg tctcgccgcg ctcgatgcgg gtgatgaagc
     2401 gccagctcta tctgagcggc gcacgcggct tgggcgaagc ggtccgcagc ggcgatcagg
     2461 aaatgctggc gagcttcgac agcgaggact ttcgcgaagg ggtggcgcat tttcgtgacg
     2521 gccgcgcgcc ggcgttcacc ggccgttgag ggtttgggcg ccggcaggat ggcggtaatg
     2581 aggtagccag gacaaaggcg aagccgcgcc tcgggatggg gcccgccgct ggcgcaaact
     2641 ggggacgcac tgtcggcgaa accgtcgatg gcggtgatgt tcagcaagcc agccgtgccg
     2701 aagagcgacg tggccccgcg ccacgaatcg ctggcggtgc cgcgccggcc ggcgcgccgc
     2761 gacaccccca tggtcgcgcc gatcgacctg gccctgctcg cggggctgcc ggttctcacc
     2821 gcgctgtcct ggctgctgcc ccccgcggca tggccggcgg tcgcgcgcgc gctgacgccg
     2881 ttccagctgc gcgagatggc ccgcgatccc gatgccctgg cgcgcgtcgt cgcgcgcatc
     2941 tgcggccccc gatcgggcga ggtggacggc cgcgacgtcg tcgagcggct cgcccggcac
     3001 gacctgatgg cgttgctgct gatgttgcgc gccctgcggc cgggcgccgc gccgccggcg
     3061 attcgcctgt cgggcggcga gcgcattgcc gctgcattgg cggacgggcg cggcgcgatc
     3121 ctgtggatcg gctatttcgc ctatgcgtcg atggtcgcca agatggcgtt gcacgaggcc
     3181 ggtcatcgga tcgtccatct cagccatccg cggcacatgt tttcgtcgtc gtggttgggc
     3241 atgcgggtga tcaacggcgt cgcgcgcaat gccgaagacc ggttcctcgg cgaacgggtg
     3301 ctgttgggcc tcgactcagc gaccgcggcg ctgcgcacgc tcgcgcacca tctcggcgag
     3361 aaccgggtgg tcagcgtcgc cgcacgcagc acggccacgc ggccggtccg ggtcggcttc
     3421 ctcggccgcg agctggcgct ggcgccgggc gcgctcgacc tcagctttcg caccggcgcc
     3481 ccggtgctgc cggtatttac ggttcgcgcg cacgacggct tcgaggtggt ggtcgagccg
     3541 gcgctgacgg tcgaccggtc gctgccgcgg cgccgggcca cgcgcgaggc cgcgcaagcc
     3601 ttcgcccgcc tgctcgagtc gtgggtgctg agccatccgg accaatggca ggactggcag
     3661 cacctgtagc gtcaggcacg gccgcgaagc gcgcgcgcct ccgcccgatg ccaggcgagc
     3721 aggcccggcg cgccgatcag gagctcgcgc ccgcgggtgg cgagcgagac cgcgagcagc
     3781 gccgccggcg aggcgtcgac cacggcggcg agcgcgacat accccgcctc ctggatgccg
     3841 atgccgccgg gaaccatgaa cagcaggccg cgggcggcgc cggtcaggct cttgaccatg
     3901 agcgcgtccg tgaacgtcag cgacatgccg atcagatagg ccgccagcca cacctcgagg
     3961 gccagcgcga cgcgtgccgc gaggtggagc gcgagcgcaa gcgcgagccg ccgcgggcgg
     4021 gcgtagatgc gcctgagcgc agcgtccagg cgcagcccgc cgcttttcgc cgtcataccg
     4081 tcgccgccgc gcacggcccc ggcgaacgcg cccgcgaccc tgccggcgac gccagcgtgc
     4141 tggaccgcaa cgaatcccaa ggtgccggcc acgaccaggc cggcggcgat ggtgaccgcg
     4201 atggccagcg cggattcgcc ggtgcgcgcg gcaagcagtc ccgcgccgag cagcgcccac
     4261 gccgccagcg acagcgcctg caccgcctgc ccggcgatga ccgaggcggc ggcgtcacgc
     4321 gcgcggatgc cgtcgagcgc gaccaggcgt gccctgaccg cctcgccgcc gagccccgtg
     4381 accggcaaca acaggctgac cgagttgccg atccaggtcg cgcgcagcgc cgccgcgagg
     4441 ctcggctcgc gccgcggccg gaacagcggt cgccacgacg ccgcggcaag acccaggctc
     4501 ggcagatagg ccggcggcaa cagcagcagg ccccagccga gcgcgaccag ggcctgggcg
     4561 accggctcta tgccgcgcca gacgacgagc gcgaacatga gcgcgatgcc ggcgaccagg
     4621 ccgaggcgcg taagcacccg caatcgccgc cagccgccgc gcggcggcct ttcgcctgcc
     4681 gcccgatcgg tgacgacgtg gcgctctgcg tcctgtccga tcggtctcac gctcgcggca
     4741 ggacgccggt gcgccggtca gcgcgcctgc tgttcaaatt ctgcgcgcgg cgggcgcgtc
     4801 atgcctccgc cgcggccgtg aacagcccga tcggcgcgcc ggacgggtcg gtgacgatcg
     4861 agatccgccc gacccccggg atgtcgaacg gcgccttcat caccttgccg cccaggccct
     4921 cgaccttggc ggtgcgggca tcgacgtcgt cgaccatgac ataggcgagc cacatcgacg
     4981 gcccggcgcc gtctgccggc ggcttcatca tgccgcccac gtcttgcgcc ccgttgttga
     5041 acagcgtgta gttgcccgtg cccatgtcca tgtcgcgccg cgtccagccc agcagcttgc
     5101 catagaacgc gccgcagccg tcggtgtcgg gcgtgatcag ctcgttccag ccgaatgtcc
     5161 cgtgtccagc catcgtctcc tccctcgcat gccggaccag ccggcgacgg tcagatctcg
     5221 ccgatccacg gatgcgcatc gcccagcatg cggtgcaggc gcgaatcgtg ggtcttgaaa
     5281 tggtcggtga accacgccgc cagcgcgctc gacagccggt cctggtagtc gtcataggcg
     5341 ccggcctcga agtcatccat gatgtcgcgg atctggtcga gcaggtcctc gtgctgggcc
     5401 ttgtgctcgc catactggtc gtaggcgcgc tcgcgcatga tcgcctcctc gagcgcgaaa
     5461 tgcgccgaga tgcgcgcgta gatctcgccg aagaaggcga tcaccgagtc gcgtcctgcc
     5521 gcagtctcga gcccggcgtg gagctcgttg atcagcgcga tcagctcccg gtgctcgtga
     5581 tcgaccgcgg ggatgccgag gctgaactcg ggtttccatt cgaccagggt catggcggtc
     5641 acctcgcgcg ctcggctgcg accggccggg aatcgaacgt gaagccgccg agctcggcct
     5701 tgaagtcgac cacgaaccgt gcgcccgcgg caagggtcac ggtctgctcg gcggcgtagt
     5761 cgaagcccga ctcgcgctcg gtgtcgcgca gcgcggcggc gatcagatgg cgccccgcgg
     5821 gcaccgtgaa gcgccggtag acgcgcgacg ggccgtcgcc ggcgatcccg ccgggcggca
     5881 gcgcgtgctc gaacatcacc tgctcgtcaa gtgccaggcg caccgtcacc ggcacccgct
     5941 cgcgcgggca gatctcgggc cggcgcatgt tgggcgccag ggcggcgatc tcctcgggcg
     6001 tcaggcgccg gcagtctccc ttgcgcgcgg caccgtgcga gaagctgagc acgagctcgg
     6061 cgctgtcggc ggggaagccg cgataggcgg gggtgttcga gaacgtgccg agcacgagcg
     6121 cgaccagggc gtagaccacg ccctggccca gatatgcgcc gagcctagcc atgctcgccg
     6181 atcggctcgg cccggcaccg cggtcgcggc tggatgcggt cgcggaacga cgcgatcgcg
     6241 gcggccaggc gcgcgccctc gctttgcgcg gcccacaccg tcagcaggcg cccgcgcggc
     6301 acccgacgcc tgagataggg atcgcgggtg cccgcgatgc gcgcctcggt ccagtcaatg
     6361 ccgccgcgat gatagcagtt gccggcgcga caaccggcga gcacgatgcc gtcggcgaga
     6421 tcgcggctga ggacgtagtc gatgaacggc ggcggcaccg cggcgatgca cggcaccgcg
     6481 accgtcgccc cgcccgcgcc gccggcgccg gcgcagtggt cgcaggcgat gaccaggacg
     6541 cgcggcgagc cggtcacggt tgcggcggcg gcgtgcaccg tctcgcgcag atcggcgacc
     6601 gagcggtcgg gcagctcgat tcccgggcgc agctcggact tgcgccggaa cggcgtcgcg
     6661 gtcgggcagg cgccggcgca gatgccgcag gcgacgcagc gcgccggatc gacgtgagcc
     6721 tggtcgcgga acggcaggcc gtctgagcgc gggcgcatca ggatcgcggc atagggacag
     6781 tcggcggcgc agcggccgca gccgttgcag ttgtcgaggt ggaccacagc cgccggcgcc
     6841 gcacgcctgg gcggcagcca cggcagcgcg accaggatca cggtcagcgc ggccgcgaag
     6901 ccccacaccg ccccggccga ccagtggtcg atcagcgggt agaacaccag gtagaaccag
     6961 tcgaggcgca gcacagaggg cacctgggcg aggtccgcgg gcgcgtggct gaccgccggc
     7021 ttgaccagcg acagcaccag cagcatcgcc agcgtgccga ccagcaggcc gcggttgggc
     7081 gtgatccgcg gccggctgat ccgctgcagg tgcagccaca gcaccagcag cagcagcagc
     7141 ggcacgacga tgtggatgaa catcagcaag gtgaagaagc ggtcgtcgag cgccgccggg
     7201 gtcaggaagt tgcgcgcgat cggctcgccg aagatgccca gccagtcgaa ccactcggtc
     7261 gagcggatgg cgatgtattg cgcgagccgg tcccacacca gccagtagcc cgagatgccg
     7321 gagatgaaca ccagccacag gatcggcgtg ccggtgaccc aggagaacca gcgcggtccc
     7381 ctgagcctgc cgagcaggaa ctcgcgcgcc gcgtggacca ccagcaccag caccatggcg
     7441 tcggaggcgt agcggtgcag gctgcgcatc accccgccca gccaccactg gtcgatggtc
     7501 aggtactcga ccgaccggta ggcctcggtg gtgccggtgc cgaacagcac gtaaacgtag
     7561 atgccgctga ccgcgatcac ccagtagaac aggaaaccga gcgcgccgag ctggtagagc
     7621 gggttccagc gggccccgaa aatgcggtcg agcagcgcct ccgcgcgttc caggcccgaa
     7681 cgcgcgatcc ggatcggggc ggtcacctca acgcaccggc tcagccatga ggcgccggcg
     7741 gcgccgcgcg cagccaggcg cggaccagca cgaaggcgat gccgccaagg ctcgctacgc
     7801 cgaccgcgag cgcgatgaag atcgagtagt cgaaccggta gcggccggtg gtcgggtcga
     7861 aataggtgca gaacaggcgc agccggtcga ccgcgtcggc gaggctcgcg atccccttgc
     7921 cgccgccgta gaccagcgcc agcaacggct cgaccaccgc cggcggctcg aactcggcgc
     7981 cgtagacctg gcggaagacg cgcccctcgg cgtcgaccac ggtggtctgg gccagatggt
     8041 cgaagccgcg cggcgagggg aagtagatga agccgagcac atccgccagc gcgcgcacgc
     8101 tcgcctcatc gccgctcagg aactcccagt tgggcaggtc cagcccctgg gcgcgggcaa
     8161 agtcgcgcat gcgcccgggc gtatcggcgt cggtatcgaa gcccaccgtg atcaccgaga
     8221 accggtcgga accgaccgca tcctgcgcga tttcgacggc gcggtacaac ctttgcacca
     8281 ccaccggaca ggatagggcg cagcccgtga acaccatgtt gacgaccagc ggcttgccgc
     8341 gaaacccggc gagccggacc ggcgtgccgt ccgagccggt aaaggcgagg tcgccgacct
     8401 cgcgcccgat cgccgcctgg ctgtaggcga tcgccgcgtc ggggtcgaac gccgagggct
     8461 cgggcgcgac cttggccgca gcgttcgcgg cgccagccgc ggccagcacg accgcggtcg
     8521 gcaccgacag ccaccgcagc caccgcacgg cgcgcatcct agccgagcag cgcccggtcg
     8581 agcagcgtcg ccccgagcag caggccaagc tggaccagcg aggcgccgaa gttcgcccgc
     8641 gccgcctcag cgcccggcgc gcgcaccagg cgcaccgact ggcgcaggaa ccaggcgccg
     8701 cccaccaccg ccccggcgag gtagatccag ccgaggccga gcgcggccgg caccagcgac
     8761 agcgccacca gcgccaccgt gtgggcgaga atgatccgcg ccgccgccga atcgcccacc
     8821 accaccggca gcatcggcac gccggcacgg gcgtagtcct cgtgcagcac cgtcgccagg
     8881 ctccagaagt gtggcggcgt ccacaggaac agcaccccgg ccaggatcag cggcacggcg
     8941 ccgaggtcgg gctcgaccgc ggccgcgccg gcaagcacgg cgaagctgcc ggcaagcccg
     9001 ccgagcacga tgttgagcca ggtgcggcgc ttgagccaga cggtgtagac gacgccgtag
     9061 acgaacgcgc cgaggaaggt gtagagcgcc gcccagccgt tgagcgccca cccggcggcg
     9121 ccgaccgcga ccgcgagcac ggcgaagatc agcgccagcc acgccggccc ggcgcggaac
     9181 gcgccggtga cgaacgggcg ggtgcgggtg cgggccatgc gcgcatcgag atcgcgctcg
     9241 acgtagtggt tgaaggcgcc ggcggccgcc gccgacagca gcaccgccag ggcgagcacc
     9301 gacgccttcc atgccggcag cgcgggagcc cccgccaccg ccatgccggc gagcgcacac
     9361 agcatgatcg cgaacgcaat ccgcagcttg aagacggtcc agacctgccc gaccaacgtt
     9421 gccatgccaa gcaccctttg ccgcgtcttg tcggttcgga tcagctcagc ggccagacct
     9481 cggacaggaa cttccagttg atgaagtagt agagcacgaa cgcggcgaag aagatcgcga
     9541 ccagggtcaa ggtcccgggc agcttgagcg tgccctcgct gccgtaggtc gacaccgccg
     9601 cgccgggggc gatgatcggg ttggccttga ccgcctcggc gtcgcgccgc ggcccgaaca
     9661 ggaccgagcc gaccacgatg accacgaaca tcaggccgcc cagccccgcg agcagcccgg
     9721 acagcccgtt caggcccatc atcaggtagg cggtgccggg atagtcccag ctcaaggccg
     9781 cgtcgctgaa ggtgatgtcc cagtggcggc gcgagacgcc gagcgtgccc gcgcccatca
     9841 tgaacagcga gatgcccgcg gcgccgatgc cgaacacgta gggctgccac ttggccatgc
     9901 gcttgaagat gatctcgcgc cggaagatca gcggcaccac cagataggtg atcgccatga
     9961 aggcaagcgt ggtgccggcg accaccgtgc cgtggaaatg gccgggcacg tagagcgtgt
    10021 tgtgcatcaa gaggttgatc tgctcggtgc cgagcaccac gcccgagatg ccgccgagga
    10081 agccgaacat gaccagcgac agcgccatgc ccgagaatgc cgggttgccc cacggcgcgt
    10141 tgcgcagcca gccgaacacg ccctgggtgt agccgcggcg gcgttgcgcc gcctcgaccg
    10201 cgccgggcac gctcatgccg tggatcatgc tgccgagcac ggcaaggtag agggcatagg
    10261 aggtgttgaa caccttccac tccgagctga tcccgggctc ggccagcatg tgatgggcgg
    10321 ccgccagctg caggaacagg atgtagagca ggaacgcggt ccggctgacc ttctccgaca
    10381 gcggcttggc gccgagcacc atggcggcga tcgcgtacca caccgcgacg tgggccgaga
    10441 cgttgatctg ctgcgaggca tgacccatgc cccaccacac cagcttgtag gtgaccgagt
    10501 cgatctcggt gatgtagccg accgaccaca ggaaggtcgg gatcaggatg atcgcgcccg
    10561 aggcgatggt gaacaccgcg atgatcgccg cggtcagcgc gccgaacgtg accagcggga
    10621 tcgagccctc ataagtctcc tcgtccttgg cgaccaccaa ggtgccgaag aacacgaagc
    10681 agccgattag cgcgccgacc gcgaacagga tcaggccgag atagaaatgc ggcgccgcct
    10741 gcatcggcac gtaggaggtg aacatcacgc tcgagtcgcc ctgcagcacg gcgaccgtcg
    10801 ccatcagcga gccgaccagc atcagcacgt agccgagcca ggcgaaggcg ggcgtcgcca
    10861 gccggcagtt gagcaggatc gaggaggcga agtagaggat cgcgatctcg aagaagatga
    10921 tccagaacag cagcaccgcc gcgccgtgcg cggtcagcgc caggtagaac cagtccgccg
    10981 gcaggatgtg gaccgccggc cagcgggtca gcgccaccag caccccgaac aggccgccga
    11041 cggcgaggaa gaccaccgcc gtgaccgcat tgacgcggat cagggtctcg gtcccggcga
    11101 acaccttgag gccggtgtcc aggcaggtgc ggtagcgcgg ttccctaaac ggtacggcgc
    11161 cttcgagcgg ttcggcggtc atggcggccc cctattcgac gacgtagatc ttgcccagca
    11221 tcgtgtggtg gccgatgccg cagtactcgt tgcagatgac tgcgtactcg cccgcctcgt
    11281 tgggctcgat ggtcagcacc atctcgtagt tgggatggac ctggatgttg atgttggccg
    11341 gctgcagcga gaagccgtgc tgccagtcca tcgacgacag gtgcagccga taggactggt
    11401 ccttttccag ctccaggatc ggataccact cccacagccg cgccctgagg tagatctcgc
    11461 tgcccggcgg cggatgcacg accgggaagc cgctatcgcc ctcctcgcgc accgtgtact
    11521 ggtcgaccat cgcctcggtg cgctcgtcga acgccgacgg gtcgacccgg taggcctcgt
    11581 tggataggtt ctgccggcca ttgatgtgcc accaaatcat tgccccgaac atcaccagac
    11641 cccagacgaa ggcgatgctg atccaggtga tctccattcg gtccaggggc tcggaccacc
    11701 agatgcgctt ttcgggcggg taaatcgcca tcgcgcgtct cctatttcga gatcggaatc
    11761 tgcgccacct ccatcacccc ccacatcagg tagaacacgg tggggatggc gacgccgagg
    11821 aacagcagca gaaacgggtt gtcgagcacc ttttgcatga ccggcacgcg cacgcgccgc
    11881 gcgccgcttt cgccggtggt tggctccgtc atggcacatg tcctccaggc cgcccggccg
    11941 accgcgtcgg ccgggccggg ttcaccccgc cgaccgggac cgccggcaag cgaattgcaa
    12001 cgcgcgcggt gccaagcgtc catgacgccg gtcaagcgcc ggtcgcgcgc gcggcgccgg
    12061 cgaccggtcg tgatggagat gttcgtgatc gagttgctcg ccggtgcgac cggcgcatcc
    12121 gggcacgacg agcgtcgccg ccggttgcgc ccgcccgcgc gcacgccggc ggacaccgac
    12181 aggatcggcc ccgggccccg cgcgggtcat tggcgcttca cggtcatcgt tcccccgacg
    12241 gcgaccacga atgctggcga ttcgcggcgc cattatacgc ccgccttgac cgcgatcaac
    12301 cgacgccgga acttgggcgg cggtcggccg gcggatgggc gcgggcgcgg tcgcaggagg
    12361 catcatgaac cggaccgacc tcgggccaga ggacatccag gtcctgcgca gccagccgct
    12421 gtttcgcggc ctcgacgggc gcgcgctcgc ggacctgctg tcggacgccc gggtgcgcac
    12481 atacccgcgc gccagcctgc tgttcctgca aggcgagccg gcggaccggt tcttcgtcat
    12541 cttcgccggc tgggtaaagc tgttccgcca gtccgaagcg ggcgaggaaa gcgtgatcgc
    12601 ggtgttcacg gccggcgaat cgttcgccga ggcggcaatc ttcgaccagg cgacctatcc
    12661 ggtcagcgcg gtggtggtcg aggaggcgcg catgctggtg gtccccggcg cctcgttcat
    12721 ccgccggctg tccgacaacg gcgcctacgc gctcaacatg atggccagca tgtcgcgcca
    12781 cttgaggcag ctggtgcagc aggtcgagca actgtcggtg cgctcctcga ccgagcgcgt
    12841 cgccgggttc ctcgcccgcc tgtgcggcga ggccgaggcc ggggccaggg tgcagctgcc
    12901 gctggaaaag gcgctgatcg ccggccggct cggcatgcag cccgagacct tctcgcgcgc
    12961 gctcgccagg ctgcgcgacc agggggtgac ctcgagcggc ggcgagatcg tgatccgcga
    13021 cgtgggcagc ctgaggcgaa tcggcggcgg cgccgtcgcc gacgggcgcc ccagccgcgt
    13081 cacgcaccga gcgttgcgcg cgtgaacgcg gcgacgtgat cgagcagggc gtccgaggcg
    13141 gcggcggcgc gctcggcgtc gccgtcggcg atggcaagcg cgatgtcgcg gtgcagatgc
    13201 gccgcctcgc gcacgctcga gcggcgcccg tgatgggcgt accagaagcg ccgcgacagg
    13261 ccctgcatca gggatgccgc ctcggccgcg aactcgttgc cggcggcgct cgtcagcagc
    13321 aggttgaagc ggcggtcggt tcgggtgaac aaggtgtcgt cgtcgccgtc gccggcctcg
    13381 gcgaacgccg ccgccgagtc gctgaagtcg gcggcctggg ccggcgttcg ccgcgccgcg
    13441 gcgcgcgcga cgaggaggcg gtcgacctcg cgccggacct cgagcatgcg cagctgcttg
    13501 ccgatgtcga ggtcggtgac cagcaggccg cggcgcggca ggatgcgcac cagcccctgg
    13561 cgggcgagcc gctgcagggc ctcgcggatc ggggttcggc tcattcccag gcgcttggcc
    13621 aatgtcgcct cggagatggt cgtccccggc gccagcgcca aggtcacgat cagctcctcg
    13681 atgtgctcgt atgcctggtc cttgaggctc tccgccgcgc ccgccggcgc ggcaatgatg
    13741 cgcgccgcct cggccattcg ctcgccctgc ctggcaattg atattcgact cgtataccag
    13801 ccgcttataa caggccccgg ccccgcggcg ccagcggctc aagccgccgc cggcgtctca
    13861 gtttcaaatt gaccgtgtgt aatcgacttg tgacgtcaat tcgttgccgt taactcgatc
    13921 gccagcttct ggatcgccaa ccggtcgtcc aaccgcgatg agcggctggg caatttctag
    13981 ccaagagcgg tcgtgcgaca gatggtgaac caactgcaaa tcaacccgcc tgcgttccga
    14041 aagagcgatc ggcgagcagt aggtgtcggt gcgcccggtg acgagtgcgg gcaggcgcgg
    14101 acactgttca tgtcttagcc catcttgtgg gccataagga tcagggccgg catgtcgaag
    14161 ctgacgatac cgtcgttgtc ggcaaagggc cgcaaggcct cctcggactc ccccagcagc
    14221 aggtcgaact ggtcctggtc aaggatgccc ccgagggtcc agacacaggc acgttctgtc
    14281 gccaccagtt ccgcgatggt ctggaagcgg acggtaccgt tgcgctcctc gactgaagcg
    14341 ccttcaattc ccgcggctgc cgcggtccgg ccaaggtcgt cggcatcgcc cagcacgaag
    14401 ggagcgcgga aggcgttgcc gacctcttcg ccgaacagcc ggtccaacag cgtggcaaac
    14461 gccgcatagc caggcgaacg ttccagcgcg ccacaaaccg ccaccgccag acgccccccc
    14521 ggcttcagga cgcgcatcat ctcggaaagg gcccgcgggc ggtcgtcaaa gaacatgagg
    14581 ccgaactggc tgatcacggc atcgaagctt gcatcgtcga aggggaggtt ctcggccact
    14641 cccacccgcc agtcgatgtc cgcggatttg cgccgcgcga cggccagcat ctcgggattg
    14701 gcgtcgaggc ccacgacaca gccgtccggc cccgccactg cggcggccgc cagcgtcagc
    14761 gcgccggtgc cgcaggcgac gtccagcacc gcttgcccag gtgcgacgcc ggccacgttt
    14821 gacaccaccg gtccccattg tccaaaaagg gcgggtacga acttctcatc atagatctcg
    14881 gccggcggcc gttccctctt catgacattc atgatgacgc ctttccgttc gcggtttccg
    14941 gtcgtactct tcaaatcggt ccatggcccg gaagcctaaa ggagagccat gccgcccgcc
    15001 catgaccagc cgtccgaaac gcttgccggg gcgtccgaaa cgcacgtagc ggcgcgggtc
    15061 ggccgttacg atccgctttc ggacgtgctg cggacagtga agctcaaagg cgcgctgttc
    15121 ttcctggtcg acgcaacgag tccgtggtgc gtcgacattc ccccggcaag agatttcgct
    15181 gacatcatcc tgcctcgcgc acggcatgtc gtctcttacc acgtgatcgt ggagggccac
    15241 gggctggcca gcgtgccacg aatcgacccg gtccactttg atgccggcga tatcatcgtc
    15301 ttcccccacg ccgatcccta tctcatgtcg agcgcaccgg gcgtcccgcc ggaactggac
    15361 cgcaggcaaa cactgcagtt cttccgcgac ctcgccgccg ggaaattgcc gttcgtcatc
    15421 cccgaaggcg gcggcaagct cccgaaggca aagttcattt gcggctttct gggctgcgac
    15481 ctgagtcctt ttaacccgat attcgccacc cttccgcgga tcctgcacat tcgccgcccg
    15541 agcggcgatc atgcggacct ccttgaccag ttgattgacc tcgccatggt agaggtaggg
    15601 acgacgcgcg ccggcggtga gagcattcgg ctgggcctga gtgagctgat gttcgttgaa
    15661 ctgttgcgcc gtcatctcga aacgctttcc ggggacgcgc caggttggct ggcgggcctc
    15721 cgtgatccca acgtcgggcg cgcgctggcc ctgcttcact ccgagccggc aagggattgg
    15781 acgctcgttg cgctggcccg tgcggccggc gtctcccggt cggtattggc agaacgcttc
    15841 gcccgttgtg tcggcgagac accgatgcgc tatctggcac tgtggaggat gcagcttgcc
    15901 gcacggctgc tggtagacgg cgccacaaag gtcgcggtcg taggcgaaca ggtcggcttc
    15961 cgctccgaag ccgccttcag ccgtaccttc aaaagagtaa ccggcttttc cccagcggaa
    16021 tggcgccgca caagcgcaaa tcccttggcg cgaggtccgg gacgctgact tggggcatga
    16081 ttggtcagcc gacggcaccc ggctccggat cacccttcgg cgcgcgggcg atcgcgagtg
    16141 ccccgttgcc cgtcgcgcgg ttcggtgtca cgatcgatcc gcggcgaggt gatcgggcgg
    16201 gacatcgcga aagaagggcg ctccgtccgt gtggccccgg ctccgatgcg aaactgaccc
    16261 cgagaaggat gttcgcggtc atggcaggtc gacttcgagg cctcgcgcct gcgatcgcgc
    16321 tggcggcgat catcagtgcc ggcccggtgc cggagagcgc agaggccgac tcccgctcct
    16381 ccacctcgat caacgggcgg acgatcaccg tggtcggcgg gtcgagccag tcgatcagct
    16441 cgacggctga gcggaccgtc gtcaagctcg acggctcgcg gatcgtgatc cgcgacgggc
    16501 ggatcgagct cgaggacgcg gtccgtgtgg tcggcgactt caccaagctg gagattcgtg
    16561 cccgggacgg ccgcatcgac atcttcgtcg acggcgagcc gatcgacgag gcgatcgagt
    16621 aaggcacgtc cggggcccga cctcggagac ccgccggccc ggcgaaagcg ccttgccaag
    16681 ggccgaagtt tgcgcgatca ttgctggtat cccagttgta ttcatagtcg ccggcgcgcc
    16741 ggccgcgccg cgcgggggat gtttatgggc cgcatcgagg ttgtcggcag gcgcgtcgag
    16801 ctggtgtcga tggaccccca tttccacgac atctcgatcg cgctcaaccg gctcgacagc
    16861 gacgacggct gcgcctacct ggtccacacc tacagcggcc gcgacggcgc cggcgcgcgg
    16921 atcgcaagcg tggtggcggc gatgacgacc atgggcgaca tggaagcggt gccgggcgat
    16981 gcgcgcctgc tgcgctttcc gtgccgggcc gcgcaccagt tcgcctgccg gcggctgttc
    17041 ctcgaggcgt gcaagatcgc cccgggcgcc gcggccgacg ccaagccgct cgcggtcctc
    17101 gacaggaagt ccgcgcacac catctgcggc gagagcctcg gcggcggcgc ctacagcttc
    17161 ggcctcgagg gcggcggctc gggcggcgcc gagcgcgcgg cagtgctcgc gcgcggcctg
    17221 gtcaagctcg ccgagctcga cgccgccgac gaccagccct accgggccgc cttcgcctgc
    17281 ggccaggcgc acgacgccct gatcggtctc ttgatggcgc gcgcgctcaa cgtccgcgcg
    17341 gcgctgcgcg aggtcgaggc ccaggccggg cgcggcgtgc tcgccgcacc cagcgcccag
    17401 aagcagtgac cttcggccag cggaggacag acctatgacc gacacctctc aatcacgccc
    17461 cgcctcgatg accagccgcg agcgcgtgct cgccgcgctc aggcgcgcgc cggtcgaccg
    17521 cacgccggtg tgcaacccga cctcggtcgc gaccgtcgag ctgatggacc tggtcgatgc
    17581 gccgttcccc gacgccaacc gcgatcccga attgatggcg cggctggcgg cgaccggcca
    17641 taccgagctc ggcttcgatt cgatcatgcc ggtgttctcg atcatccagg aatcgtccgc
    17701 gctggggtgc aagatgcagt gggagcagaa ggacaactgg ccgaccgtgc gcatgcgcga
    17761 gccgatcttc cgcgagcccg acgacatcgt catccccaac gacctgctca cccacccgga
    17821 caccgcctgc gtgaccgagg cgatcaggat tctcaagcgc gagttcggcg acgaggtcgc
    17881 gatcatcggc aagaccatgg ggccgtggtc gctcggctac cactgcttcg gggtcgagcc
    17941 gttcctgctc atgtcgctcg acgatcccgg caagaccaag acttgtctcg accggctcaa
    18001 ggaggcgacg gtccagttcg ggcttgccca gatcgaggcc ggcgccgacg cgctgaccct
    18061 tcccgaccac gccaccggcg atctggtgag cggcgaatat tacgcccgct atctgaagga
    18121 catccacatc gagttcgccg agcgcctgcc ggtgccgctg atcctgcaca tctgcgggcg
    18181 cacggtcgac cgcatgccct tcatcgccga gaccggcatg gccgcgttcc actacgattc
    18241 caagaacaag cccggcgagt ccatggccgc ggtgcatggc ggcatcgcgc tggtcggcaa
    18301 catcaacaac cccgagaccc tgttttccaa ggggccggcg gaggtgcgcg ccgaggtgtt
    18361 ccgcaacctc gaggccgggg tccagctgat cgggccggaa tgcgcgatcc cgctgcagac
    18421 gccgctcgag aacctgatcg agatcccgcg cgcggtgcgc
//
LOCUS       DASWHU010000175         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3396913, whole genome shotgun sequence.
ACCESSION   DASWHU010000175 DASWHU010000000
VERSION     DASWHU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3396913"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..921
                     /locus_tag="VGB88_06580"
     CDS             73..921
                     /locus_tag="VGB88_06580"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9647145.1"
                     /translation="MSIEIRPLSAALGAEVTGLDLTEPLAAADRDAVIAAFLDRHLLC
                     LRSAPLTAGEFARTARTFGTPQLQLLRSYRHAEVPEVSVLDSTYKTLKDKPDDLARVR
                     LSGWHTDDSYFEVPAKATMLQALKIPQSGGETRFCNTRKAYDDLPDATKRRIDGLKAV
                     HAYDTMRAPARAAVLTNEEASETPDVVHPLVRTHDDTGAKAIYLNSNRTDSVVGLARA
                     ESDALLDTLHAHMTRPVYRYDHRWRVGDILLWDNRCLIHSVNVDFPVGEARVHQRILL
                     KGTRPA"
     gene            918..>2359
                     /locus_tag="VGB88_06585"
     CDS             918..>2359
                     /locus_tag="VGB88_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX9647146.1"
                     /translation="MTGARGANAPINAGGALLMRLKALGVEYLFANSGTDFPPLIEAI
                     AQAAEQGLAMPEPVIAPHENAAMGMAHGYYLATGRPQAVMVHTNVGLANAAIGAINAA
                     CDHVPVVLMSGRTPVTEHGRLGARGHPISWGQEMRDQAALVRECVKWDFELRFPEQVA
                     ELADRALAIAMSLPRGPVYMSLPREVLCATCPAEALANPPHLAPAIPAPRAGDVARAA
                     EFLAGAARPLVVAQRGPGSAQGFATLARLAEAWALPVVQFWPTHLSLSWDHPMHAGFD
                     PAPWLADADAVLVIDALTPWLPSECALPADCPVVQVGQDPLFSRFPVRGFRADVALTG
                     EVPDVLAALEAALAPALAGAGARIAARREDIVARNRAAAAKRDATAKAGAGRPMTKAW
                     ASRCVAEAVEAHDGIALSELGCEPPFMRLTRPGSLLLEPHSGGLGWGFPAALGLCLAD
                     RDRLVVASVGDGAYMFANPVACHQIAESLAL"
BASE COUNT          308 a          894 c          833 g          324 t
ORIGIN      
        1 cctgcgagcc cgctggaacg gcggcggggg atgctctagt ctttggtccg gcggcggacg
       61 gaggaggcgg gcatgtcgat cgagatacgg ccgttgagcg cggcgctcgg cgccgaggtc
      121 acgggcctcg acctgaccga gccgttggcg gcggcggacc gcgacgcggt gatcgcggcg
      181 ttcctcgacc gtcacctgct gtgcctgagg tcggcgccgc tcaccgcggg cgagtttgcc
      241 cgcaccgctc gcactttcgg caccccgcag ctccagctcc tgcgcagcta ccgccatgcc
      301 gaggtgcccg aggtctcggt gctcgacagc acctacaaga ctcttaagga caagcccgac
      361 gacctcgccc gggtacgcct ttcgggctgg cacaccgacg actcctattt cgaggtcccg
      421 gccaaggcga ccatgctcca ggcgctgaag atcccgcaaa gcggcggcga gacccggttc
      481 tgcaacacgc gcaaggccta tgacgacctg cccgacgcga ccaagcggcg catcgacggt
      541 ctcaaggctg ttcacgcgta cgacacgatg cgtgcgccgg cgcgcgccgc cgtgcttacc
      601 aacgaggaag cctcggagac cccggacgtg gtccacccgc tggtgcgcac ccacgacgac
      661 accggggcca aggcgatcta cctcaactcc aaccgcaccg actcggtggt cgggctcgcg
      721 cgggcggaga gcgacgcgct gctcgatacg ctccacgccc acatgacccg ccccgtttac
      781 cgctacgacc accgctggcg cgtcggcgac atcctgctgt gggacaaccg ctgcctgatc
      841 cactcggtca atgtcgactt ccccgtcggc gaggcgcgcg tccaccagcg catcctgctc
      901 aaagggaccc gcccggcttg accggcgcgc gcggcgccaa cgccccgatc aacgccggcg
      961 gggcgctgct gatgcgcctg aaggcgctcg gcgtcgaata cctgttcgcc aactcgggca
     1021 ccgacttccc gccgctgatc gaggccatcg cccaggccgc cgagcagggc ctcgccatgc
     1081 cggagccggt gatcgcgccg cacgagaacg cggccatggg catggcccac ggctattacc
     1141 tggcgaccgg ccggccgcag gcggtgatgg tccataccaa tgtcggcctg gcgaatgccg
     1201 cgatcggcgc gatcaacgcc gcctgcgacc acgtgccggt ggtcctgatg tcggggcgca
     1261 cgccggtcac cgagcacggc cggctcggcg cgcgcggcca cccgatcagc tggggccagg
     1321 agatgcgcga ccaggcggcg ctggtgcgcg agtgcgtcaa gtgggacttc gagctgcgct
     1381 tccccgagca ggtcgcggag cttgccgacc gcgcgctcgc gatcgccatg tcgctgccgc
     1441 gcgggccggt ctatatgagc ctgccgcgcg aggtgctgtg cgcgacctgc ccggccgagg
     1501 ccctggcgaa cccgccgcac ctcgcgccgg cgatcccggc gccgcgcgcg ggggacgtgg
     1561 cgcgcgcggc cgagttcctg gcgggcgccg cacgcccgct ggtcgtcgcc cagcgaggcc
     1621 ccggatcggc acaaggcttc gccaccctcg cccgcctggc cgaggcgtgg gcgctgccgg
     1681 tggtccagtt ctggcccacc cacctgtcgc tgtcgtggga ccatccgatg cacgccggct
     1741 tcgacccggc gccgtggctc gcggacgccg atgcggtgct ggtgatcgat gcgctcaccc
     1801 cgtggctgcc gagcgagtgt gccctgccgg cagactgccc ggtggtccag gtcggccagg
     1861 acccgctgtt cagccgcttc ccggtgcgcg gcttccgcgc cgacgtggcg ctgaccggcg
     1921 aggtgcccga tgtgctggcg gcgctcgagg ccgcgctcgc cccggcgctc gccggtgccg
     1981 gcgcgcgcat cgcggcgcgg cgcgaggaca tcgtcgcccg caaccgggcc gccgccgcca
     2041 agcgcgacgc gaccgccaag gccggcgccg gccggccgat gaccaaggcg tgggcgagcc
     2101 gctgcgtcgc cgaggcggtc gaggcgcatg acggcattgc gctctccgag ctcggctgcg
     2161 agccgccgtt catgcgcctg acgcggcccg gctcgctgct gctcgagccg cattcgggcg
     2221 gcctcggctg gggcttcccg gctgcgctcg gcctctgcct cgccgaccgc gaccggctgg
     2281 tggtggcgag cgtcggcgac ggcgcctaca tgttcgccaa cccggtcgcc tgccaccaga
     2341 tcgcggagtc gctggcgct
//
LOCUS       DASWHU010000176         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1568006, whole genome shotgun sequence.
ACCESSION   DASWHU010000176 DASWHU010000000
VERSION     DASWHU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568006"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VGB88_06590"
     CDS             <1..1594
                     /locus_tag="VGB88_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEX9647147.1"
                     /translation="RLRQRQRLAELERGRAELDARHAEAAAAVEQAAERREQARAAYA
                     RAREDEAGARAAVRAADTVLERAREAHAEAAEALAEARSRHAALADARARLAADQSEA
                     ERAAAETVEALAALPGPEAGRAVLDEARARLAERRAEAETRLREHEGLVRAAEARAER
                     LRAIAAESEAWGKRARSAAEQVAALEARTRALYERLAALQGEPARLAQQRHGLQDQID
                     AATAARAGAADALAAAEGRLAAADRALRDANEALARAREERVRAEGRRDQARSLAEMA
                     AARIRERFDCDPARALALAEVDDAGALGEIEPLERQVERLHRERDNMGAVNLRADEEA
                     AELGEQIDTMRTERDDLVSAIAKLRGGIASLNREGRQRLLAAFEEVNAHFQSLFQRLF
                     GGGRAHLELSGDDDPLEAGLEVMASPPGKRLQALSLLSGGEKALAALALLFALFQTRP
                     APICVLDEVDAPLDDSNVDRFCTLLEDITTESRTRFLVVTHHRMTMARMHRLFGVTMG
                     ERGVSQLVSVDMDDAHQLQAAE"
     gene            1746..2120
                     /locus_tag="VGB88_06595"
     CDS             1746..2120
                     /locus_tag="VGB88_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9647148.1"
                     /translation="MTDGGERERETLARLGARLKRARQSSRIVPHRPGRAGPPAAGLG
                     IALRIGVEMVAGVAVGAGIGYLLDQWFGTSPWLLIVFFFLGAGAGGLNTYRAITGSGF
                     GLVSAGEAEGDEDEDGKGDRGS"
     gene            2127..2894
                     /locus_tag="VGB88_06600"
     CDS             2127..2894
                     /locus_tag="VGB88_06600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9647149.1"
                     /translation="MAAEQAAHAEGAGGHSPLAQFEIHELVPIHIGDLNLSYTNSALW
                     MTIAVALTTVLFTAGTRGRAMVPGRVQSIAELAYQFVAGMIRDNVGNAGRPYFPFIFT
                     LFMFILFGNMLGMVPYSFTYTSHIIVTFSMAVVVFVGVTVIGFARHGAGFARFFVPAG
                     VPAPLLLLLVPIEVLSYFVRPVSLSLRLFANMMAGHTMLKVFGGFVVALGALLGWAPL
                     LLIVALTGLEIGIAFLQAYVFAVLSCLYLNDALHMHH"
     gene            2941..>3098
                     /locus_tag="VGB88_06605"
     CDS             2941..>3098
                     /locus_tag="VGB88_06605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997791.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit C is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit C"
                     /protein_id="HEX9647150.1"
                     /translation="MELDAAKMIGAGLATIALAGVGVGIGLIFGNFVAAAARNPAAMP
                     KVFGNVLLG"
BASE COUNT          406 a         1111 c         1154 g          427 t
ORIGIN      
        1 ccgcctgcgc cagcgccagc ggctggccga gctggaacgc ggccgggccg agctggacgc
       61 gcgccacgcc gaggccgcgg cggcggtcga gcaggcggcg gagcggcgcg agcaggcacg
      121 cgccgcctat gcgcgcgcgc gcgaggacga ggcgggcgcg cgcgccgcgg tgcgtgccgc
      181 cgacaccgtc ctcgagcgcg cccgcgaggc ccacgccgag gccgccgagg cgctggccga
      241 ggcgcgctcc aggcacgccg cgctcgccga cgcacgcgcc cgcctcgccg ccgatcaaag
      301 cgaggccgag cgggccgcgg ccgagaccgt cgaggcgctg gccgcgctgc ccggccccga
      361 ggccgggcgc gcggtgctcg acgaggcgcg cgcgcgcctt gccgagcgtc gcgccgaggc
      421 cgagacgcgc ctgcgcgagc acgagggact ggtgcgcgcg gccgaggccc gcgccgaacg
      481 gttgcgggcg atcgccgccg agagcgaggc ctggggcaag cgcgcgcggt cggcggccga
      541 gcaggtcgcg gcgctggaag cgcgcacgcg cgcgctctat gagcggcttg ccgcgctgca
      601 gggcgagccg gcgaggctcg cgcagcaacg ccacgggctt caggaccaga tcgacgccgc
      661 gaccgccgcg cgcgccggcg ccgccgacgc cctcgccgcc gccgagggcc ggctggccgc
      721 ggccgaccgc gccttgcgcg acgccaacga ggcgctggcg cgcgcgcgcg aggaaagggt
      781 gcgcgccgag ggccggcgcg atcaggcgcg ctcgctggcc gagatggcgg cggcgcggat
      841 tcgcgagcgc ttcgactgcg atccggcacg cgcgctggcg ctggccgagg tcgacgacgc
      901 gggcgcactc ggcgagatcg agccgctcga gcgccaggtc gagcgcctgc accgcgagcg
      961 cgacaacatg ggcgcggtca acctgcgcgc cgacgaggaa gcggccgagc tcggcgagca
     1021 gatcgacacc atgcgcaccg agcgcgacga cctggtctcg gcgatcgcca agctgcgcgg
     1081 cggcatcgcc tcgctcaacc gcgagggacg ccagcgcctg ctggccgcgt tcgaggaggt
     1141 caacgcgcat ttccagtcgc tgttccagcg cctgttcggc ggcgggcgcg cgcatctcga
     1201 gctgtccggc gacgacgacc cgctcgaggc cgggctcgag gtgatggcga gcccgcccgg
     1261 caagcggctg caagcgctgt cgctgttgtc gggcggcgag aaggcgctgg cggcgctggc
     1321 gctcctgttc gcgctgttcc agacccgccc ggcgccgatc tgcgtgctcg acgaggtcga
     1381 cgcgccgctc gacgactcca acgtcgaccg gttctgcacg ctgctcgagg acatcaccac
     1441 cgagtcgcgc acccgcttcc tggtggtcac ccaccaccgc atgaccatgg cccgcatgca
     1501 ccggctgttc ggggtcacga tgggcgagcg cggggtctcg cagctggtct cggtggacat
     1561 ggacgacgcg catcagctgc aggccgcaga gtagcccgcc gcgggccgcg tgcctgtcgt
     1621 ttttcgtttc tatatcaacg agttagagta gatcgtcggc gcttgacagc accccgggca
     1681 gtgactatgg tgccgccggc ttcggggggc ggcggggtcg cgtcccggcg ccgcgggagg
     1741 tcgccatgac cgacggcggc gagcgtgagc gcgagacact cgcccggctc ggggcgcggc
     1801 tcaagcgggc gcgccagtcg agccgcatcg tcccgcaccg gccggggcgc gcggggccgc
     1861 cggcggccgg gctcggcatc gcgctccgga tcggcgtcga gatggtcgcc ggcgtcgcgg
     1921 tcggcgccgg catcggatac ttgctcgatc aatggttcgg cacctcaccg tggctgttga
     1981 tcgtgttttt ctttctgggc gcgggcgcgg gcggcctcaa tacctaccgg gcgatcaccg
     2041 gctccggctt cgggctggtg tccgcaggcg aggccgaggg cgacgaggac gaggacggca
     2101 aaggcgatcg ggggagctga ggcgaggtgg cggcggaaca ggcggcacat gccgagggcg
     2161 cgggcgggca cagcccgctg gcccagttcg agatccacga gctggtgccg atccacatcg
     2221 gcgacctcaa cctgagctac accaactcgg cgctgtggat gaccatcgcc gtggcgctga
     2281 ccaccgtgct gttcaccgcc ggcacgcgcg ggcgcgcgat ggtgcccggc cgcgtgcagt
     2341 cgatcgccga gctcgcctac cagttcgtcg ccggcatgat ccgcgacaac gtcggcaacg
     2401 ccgggcggcc gtacttcccg ttcatcttca ccctgttcat gttcatcctg ttcggcaaca
     2461 tgctgggcat ggtgccgtac agcttcacct acaccagcca catcatcgtc accttctcga
     2521 tggcggtggt ggtgttcgtg ggcgtcaccg tgatcggctt cgcccgccac ggcgcgggct
     2581 tcgccaggtt cttcgtgccc gcgggcgtgc cggcgccgct gctgctcctc ttggtgccga
     2641 tcgaggtgct gtcctacttc gtgcgcccgg tcagcttgag cctgcggctg ttcgccaaca
     2701 tgatggcagg gcacaccatg ctcaaggtat tcggcggctt cgtcgtcgcg ctcggcgcac
     2761 ttctcggctg ggcgccgctg ctcttgatcg tggcgctgac cgggctcgag atcggcatcg
     2821 ccttcctgca ggcctatgtg ttcgcggtgc tgtcgtgcct gtatctcaac gacgcgctgc
     2881 acatgcacca ctgacccggc ctgtcgcccg acaggaccaa gcgaggaaag gactccagac
     2941 atggaactcg acgccgccaa gatgatcggt gccggcctgg ccacaatcgc gctcgccggc
     3001 gtcggcgtcg gcatcggcct gatcttcggc aacttcgtcg ccgccgcggc gcgcaacccg
     3061 gccgccatgc ccaaggtgtt cggcaacgtg ctgctggg
//
LOCUS       DASWHU010000177         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3051889, whole genome shotgun sequence.
ACCESSION   DASWHU010000177 DASWHU010000000
VERSION     DASWHU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3051889"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGB88_06610"
     CDS             <1..556
                     /locus_tag="VGB88_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9647151.1"
                     /translation="EIIRMFNAAFDSLTGNRLDFYPAPLRAEIDAVNAEVYANVNNGV
                     YRAGFASRQAAYEQAFDALFATLDRIEARLTRQRYLCGTRLTEADWRLFTTLIRFDAV
                     YVGHFKCNLRRIADYPALSTYLRELYQLPGVAATCDLDHIKRHYYQSHTTINPTGIVP
                     KGPALDFVAPHDRTRLPAQGWPAG"
     gene            complement(760..1371)
                     /locus_tag="VGB88_06615"
     CDS             complement(760..1371)
                     /locus_tag="VGB88_06615"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HEX9647152.1"
                     /translation="MSLDDVETIFLAFSFIVPGFVAHATLALFLPRRAEAPQLTLIRF
                     LTLSAVNYAVWSWLLYLVVTTSFFQDRNALAAIAWVWVMLGAPVLLGLVLAQGLKRRQ
                     LYGLLQRLGLRPVHVIPTAWDWRFDRMVRPHWVLVTLQDGSSVAGLFGTDSFASSDPA
                     ERDIYIEQIYEISAAGVWSRASEGKGILVPAREIRHIEIYPVE"
     gene            complement(1448..2428)
                     /locus_tag="VGB88_06620"
     CDS             complement(1448..2428)
                     /locus_tag="VGB88_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647153.1"
                     /translation="MSRPLIAFLWPEADFRATGLDVPDGFDIRFARGTVRAEAEAASR
                     GADFLVAGAGFTAVDRALLDLAPGARLVQLTGAGTDNVDHGECARRAIPVCNVPGLNA
                     PSVAQLVVQLALRCRRPLPLLTRGGAEEWNEARARHAASGHELAGRVGVVGYGNIGRA
                     VAALFSGLGLEVVRAARAGQDDAAVPALPLDELLAGADIVAVCLPARADTRGLIDAGR
                     IARMKRGAVLIHVGRGGVVDDGAVAAALRDGRLAGAGFDVFETEPLAPDHPFLALPEA
                     ARARLVLTPHIGGQTLESKARNFAVALDNVARVARGEAPRFRVPPPAGEP"
     gene            2524..>3418
                     /locus_tag="VGB88_06625"
     CDS             2524..>3418
                     /locus_tag="VGB88_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9647154.1"
                     /translation="MRPLDDLRVLDFTTLLPGPFATLILAEAGAEVIKIEPPGGDPVR
                     HRAPRLGESSAYFAALNRGKQSIEIDLKQPGAAARLSPLIEGADVLVEQFRPGVMDRL
                     GLGYDAVKALKGDIVYCSITGYGQHGPLAQEAGHDLNYAAATGLLSMTRGHDGAPVLP
                     QTQIADIGAGSYPAIISILVALWRRARTGEGCRLDISMTDNLFPFLWYGLVQGHATGD
                     WPEGGDTPVTGSSPRYQIYATKDGRYLAVGAREQKFWDAFCDAIGLEAALRDDTRDPA
                     ATMAAVAARIAARTADHWRRRF"
BASE COUNT          575 a         1242 c         1121 g          480 t
ORIGIN      
        1 cgagatcatc cgcatgttca acgcggcgtt cgattcgctc accggcaacc gcctcgactt
       61 ctacccggcg ccgctcaggg ccgagatcga cgcggtcaac gccgaggttt acgccaacgt
      121 caacaacggc gtctaccgcg ccggcttcgc ctcccggcag gcggcctacg agcaggcgtt
      181 cgatgccctg ttcgcgaccc tcgaccgaat cgaagcgagg ctaacgcgcc agcgctacct
      241 gtgcggcaca cggctcaccg aggccgactg gcggctgttc accacgctca tccggttcga
      301 cgcggtctat gtcgggcact tcaagtgcaa cttgcggcgc atcgccgact acccggcgct
      361 gtcgacctac ctgcgcgagc tctaccagct gccgggggtg gccgcgacct gcgacctcga
      421 ccacatcaag cgccattact accagagcca caccacgatc aacccaaccg ggatcgtgcc
      481 caagggcccg gcgctcgact tcgtcgcgcc ccacgaccgc actcgccttc cggcccaagg
      541 ctggcccgcg ggatagggct tcaggatttc tgacgagtgc tatgcgtctt tcccgaattc
      601 gcaggcggct ttacgcttgt gcctccggag ggtggtttta ggctcgtggg cttcaccggc
      661 gaggtaggct tatagcctct ctcaacccgt tgcggttggt acccaagtac ccgaccagat
      721 tgcgacgctc tcttgtcgga cggcataagc atgctcccgc tattcgacag gataaatctc
      781 gatatggcga atttcccgtg cgggcacaag tatccccttg ccctcagagg ccctcgacca
      841 cacgcccgcg gccgatattt cgtagatctg ctcgatgtag atatcccgct cggccgggtc
      901 ggacgaggcg aacgagtcgg taccgaacaa gcctgcaaca gagcttccat cctgtagcgt
      961 caccagaacc caatgcggcc taaccatccg gtcgaagcgc cagtcccaag ccgtcgggat
     1021 gacatgaaca ggccgcagac cgagtcgctg gagcagaccg tagagctgcc gccgtttcaa
     1081 cccctgcgca agcacgaggc cgaggaggac aggggcgccc agcatgaccc acacccaggc
     1141 aattgccgca agcgcgttcc tgtcctggaa aaagctggtc gtgactacga ggtacaaaag
     1201 ccaggaccag accgcataat tgacggcgct cagtgtcagg aaccggatca gcgtgagctg
     1261 cggcgcctcg gcgcggcggg gcaagaacag ggccaacgtg gcatgcgcga cgaatccggg
     1321 aacgatgaat gaaaatgcca ggaaaatggt ttccacatcg tccaacgaca tcttgtccgc
     1381 ccccattcga gaaaccacgc gcttcaacga gtataaggtt acccgagagc gcgtttttca
     1441 tttttcatca tggctcaccg gctggcggcg ggacgcggaa acgcggcgcc tcgccgcgcg
     1501 cgacgcgggc gacgttgtcg agggcgacgg cgaagttgcg cgccttcgat tccaaggtct
     1561 ggccgccgat atgcggggtg agcacaagcc tggcgcgcgc cgcctcgggc agtgccagga
     1621 acgggtggtc cggcgccagc ggctcggtct cgaacacgtc gaagccggcg ccggcgagcc
     1681 tgccgtccct gagcgccgcc gcgaccgcgc cgtcgtcgac caccccgccg cgcccgacat
     1741 ggatcagcac cgcgccccgc ttcatgcgcg cgatgcgccc cgcgtcgatc agcccgcgcg
     1801 tgtccgcgcg cgccggcagg cacaccgcca cgatgtcggc gccggccaat agctcatcga
     1861 ggggcaacgc cggcaccgcg gcatcgtcct ggccggcacg ggcggcgcgc accacctcga
     1921 gcccaaggcc gctgaacagc gccgccaccg cgcgcccgat attgccgtag ccgaccacgc
     1981 ccacccgccc cgcgagctcg tggccggatg ccgcatggcg ggcgcgcgcc tcgttccact
     2041 cctcggcccc gccccgcgtc aagagcggca gcggccggcg gcagcgcagc gcgagctgga
     2101 ccacgagctg cgccaccgac ggcgcgttca gccccggcac gttgcacacc gggatggcgc
     2161 gccgcgcgca ctcgccgtgg tcgacgttgt cggtgccggc gccggtgagc tgcaccagcc
     2221 gcgcgcccgg cgcgaggtcg agcagcgcac ggtcgacggc ggtgaagccg gcgccggcga
     2281 ccaggaagtc ggcgccgcgg ctcgcggctt ccgcctcggc gcgcacggtg ccgcgcgcga
     2341 agcggatgtc gaagccgtcg gggacgtcga gcccggtggc gcgaaagtcc gcctcgggcc
     2401 acaggaaggc gatcagcggc cgggacatgg cggcgagact agcccggcgc cggcgcgctc
     2461 gccaaggccg ggcgggagcg ctaaaacttg gccctactgc gcccctgcaa gccggagcca
     2521 ccgatgcgac cgctcgacga cctgcgcgtg ctcgacttca ccacgctgtt gcccggcccg
     2581 ttcgcgaccc tgatcctggc cgaggccggc gccgaggtga tcaagatcga gccgcccggc
     2641 ggcgaccccg tgcgccaccg cgcgccgcgg ctgggcgagt cgagcgccta cttcgccgcg
     2701 ctcaaccgcg gcaagcagag catcgagatc gaccttaagc agcccggcgc ggcggcgcgg
     2761 ctcagccccc tgatcgaggg cgccgacgtg ctggtcgagc agttccggcc cggggtgatg
     2821 gaccgcctcg gcctcggcta cgacgcggtc aaggcgctca agggcgacat cgtctactgc
     2881 tcgatcaccg gctacggcca gcacgggcct ttggctcaag aagccgggca cgacctcaac
     2941 tacgccgccg cgaccgggct gctgtcgatg acccgcggcc atgacggcgc accggtgctg
     3001 ccgcagaccc agatcgccga catcggcgcc ggcagctatc ccgcgatcat cagcatcctg
     3061 gtcgcgctgt ggcggcgcgc gcgcaccggc gaaggctgcc ggctcgacat ctccatgacc
     3121 gacaacctgt tcccgttcct gtggtacggg ctggtccagg gccacgccac cggcgactgg
     3181 cccgagggcg gcgatacgcc ggtcaccggc tcaagcccgc gctaccagat ctacgccacc
     3241 aaggacggcc ggtacctcgc ggtcggcgcg cgcgagcaga agttctggga cgcgttctgc
     3301 gacgcgatcg ggctcgaagc ggcgttgcgc gacgataccc gcgacccggc ggcgacgatg
     3361 gccgcggtgg cggcgcggat cgccgcgcgc actgcggacc actggcggcg acgcttcg
//
LOCUS       DASWHU010000178         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1568687, whole genome shotgun sequence.
ACCESSION   DASWHU010000178 DASWHU010000000
VERSION     DASWHU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568687"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /gene="ftsA"
                     /locus_tag="VGB88_06630"
     CDS             complement(<1..948)
                     /gene="ftsA"
                     /locus_tag="VGB88_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9647155.1"
                     /translation="MAARAQQLIAALDVGTAKICCLIAEDDGTGVLHVLGVGHKRARG
                     LRNGAVVDMDAAEEAIRAAVHAAETMAGETIRQVVVNLSSGWPESVRFRVEVAIGGHE
                     VGDGDLRRALDQGLRFSAPEDRTVVHAIPVAYSVDGVGGIHDPRGLYGDRLEVTMLVV
                     TAGRGAVRNLSACIARCHLEIDDLVLSANAAGRAVLVEDELELGAVCIDMGAGTTSIG
                     AFLGGEMIYADCVPVGGGHVTSDIARGLSTPLEAAERLKTLYGSAIAGASDDRRVIDV
                     PEVGEGDEAGPNQISRARLVEIVHARIEETFELVGDRLGA"
     gene            complement(985..1917)
                     /locus_tag="VGB88_06635"
     CDS             complement(985..1917)
                     /locus_tag="VGB88_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX9647156.1"
                     /translation="MPRLTTRKSDRVVPSTSRAGKRRAKVRARRPAQGRSRRRRWVGW
                     LAPLGRRRSLAALALLGLAGGAAYGWASGLVARALAEAGRAAVAATADAGLVVREILV
                     EGRVHAPPEALRAALGVGRGDPLLAVDAAAVRARLEALGWVRRAAVERRLPDMLYVRL
                     VERRPVALWQHRRKLHLVDSAGTVITDQGLEAFARLPLVVGDGAPQALPALLRVIAGA
                     PELARRLSAAVRVGGRRWNLRFDDRVDVKLPEQDIAQAWARLVALQRDEQVLDRAIAV
                     LDLRREDRVVVRAKAGAAVPAGDAPRRAGKGSDA"
     gene            complement(1905..>2098)
                     /locus_tag="VGB88_06640"
     CDS             complement(1905..>2098)
                     /locus_tag="VGB88_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX9647157.1"
                     /translation="CHGVTRADFRYDDSDGGLGLRLLEINTQPGMTPMSLVPEIAAHV
                     GIGFPALVAWMVENARCHG"
BASE COUNT          261 a          815 c          773 g          249 t
ORIGIN      
        1 cgcgcccagg cggtccccga ccagctcgaa ggtctcttcg atgcgggcgt gaacgatctc
       61 gaccaggcgg gcgcgcgaaa tctggttggg tccggcctcg tcgccctcgc cgacctcggg
      121 aacgtcgatc acccggcggt cgtcggaggc gccggcgatg gcgctgccgt agagcgtctt
      181 gagccgctcc gcggcctcga gcggagtcga caggccgcgg gcgatgtcgc tggtgacgtg
      241 gccgccgccg accggcacgc agtcggcgta gatcatctcg ccgccgagga acgcgccgat
      301 cgaggtggtg ccggcgccca tgtcgatgca cacggcgccg agctcgagct cgtcctcgac
      361 cagcaccgcc cgtccggcgg cgttggccga cagcaccagg tcgtcgatct ccaggtggca
      421 gcgggcgatg caggcggaca ggttgcgcac cgcgccgcgg ccggcggtga ccaccagcat
      481 ggtcacctcc aggcggtcgc catagagccc gcgcgggtcg tggatgccgc cgacgccgtc
      541 gacgctgtag gcgaccggga tggcgtggac tacggtgcgg tcctcgggcg ccgagaaccg
      601 gaggccctgg tcgagcgcgc ggcggaggtc gccgtcgccg acctcgtggc cgccgatcgc
      661 gacctcgacg cgaaagcgca ccgactcggg ccagccgctc gacaggttca ccaccacctg
      721 gcggatggtc tcgccggcca tggtctcggc ggcgtggacc gcggcgcgga tcgcctcctc
      781 ggcggcgtcc atgtcgacca cggcgccgtt gcgcaggccg cgcgcgcgct tgtggccgac
      841 gccgagcacg tggaggacgc cggtgccgtc gtcctcggcg atcaggcagc agatcttggc
      901 cgtgccgacg tcgagcgccg cgatcagctg ttgcgcgcgt gccgccacct tagccgtgcg
      961 cctccccgca tttgtgcgcc gcggtcatgc gtcggagccc ttgccggcgc ggcgcggcgc
     1021 atcccctgcc ggcaccgcgg cgccggcctt ggcgcgcacc accacgcgat cctcccggcg
     1081 caggtcgagc accgcgatcg cgcggtcgag cacctgctcg tcccgttgca gcgcgaccag
     1141 ccgcgcccac gcctgggcga tgtcctgttc cggcagcttg acgtcgacgc gatcgtcgaa
     1201 gcgcaggttc cagcgccgcc cgccgacccg caccgccgcc gacaggcgcc gggcgagctc
     1261 gggcgcgccc gcgatcacgc gcagcagcgc cggcaacgcc tgcggcgcgc cgtcgccgac
     1321 caccagcggc agtcgggcga acgcctcgag tccctggtcg gtgatcaccg tgccggcgct
     1381 gtcgaccaga tggagcttgc gccggtgctg ccagagcgcg accgggcggc gctcgaccag
     1441 gcgcacatag agcatgtcgg gcaggcgccg ctcgaccgcc gcccggcgca cccagccgag
     1501 cgcttcgaga cgcgcgcgca ccgccgccgc gtcgacggcg agcagcgggt cgccgcgccc
     1561 gacgccgagc gccgcgcgca gcgcctcggg cggcgcgtgc acgcgcccct cgaccaggat
     1621 ctcgcgcacc accagcccgg catcggcggt ggcggcgacc gccgcccgcc cggcttcggc
     1681 gagagcgcgc gcgaccaggc cgctcgccca gccataggcc gcgccgcccg cgagcccgag
     1741 cagcgcgagt gccgccaggc ttcgccggcg cccgagcggg gcgagccagc cgacccagcg
     1801 ccggcgccgc gaccggcctt gcgccgggcg gcgcgcgcgc accttcgccc gccgcttgcc
     1861 ggcgcgggag gtcgagggga ccacgcggtc cgatttgcgc gtcgtcagcc gtggcaccgc
     1921 gcgttctcca ccatccacgc gaccagggcc gggaacccga tcccgacatg ggccgcgatc
     1981 tcgggcacca gcgacatcgg cgtcatgccg ggctgggtgt tgatctcgag caggcgcagg
     2041 ccgaggccgc cgtcgctgtc gtcgtagcgg aagtcggcgc gggtcacgcc gtggcagc
//
LOCUS       DASWHU010000179         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_455432, whole genome shotgun sequence.
ACCESSION   DASWHU010000179 DASWHU010000000
VERSION     DASWHU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455432"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VGB88_06645"
     CDS             complement(<1..650)
                     /locus_tag="VGB88_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9647158.1"
                     /translation="MTRAAESIDSWIGKSRETEDLIAPFQARAMAATLDHARAPAEGD
                     ALPPGWHWLYFPDTPRPGALGPDGHEARGGFLPPIPLPRRMWAGNRLVFHQPLRVGER
                     ARRHSVIERIERKEGRAGALAFVTVHHRYSGAAGLALEEWHDIVYRAEPAPGAALPPG
                     KGAPAKARWRREITPDEVLLFRYSALTFNGHRIHYDYPYVTGEEGYEGLIVHGPLTA"
     gene            complement(647..1774)
                     /locus_tag="VGB88_06650"
     CDS             complement(647..1774)
                     /locus_tag="VGB88_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9647159.1"
                     /translation="MAGGSLDGLLVVTLEQAVAAPLCSARLADAGARVIKVERAEGDF
                     ARAYDSVVNGDSAYFVWLNRGKESLILDIKNQEDIFLIKRIISKADVYIQNLAPGAAA
                     RAGIGTAEMRARHPRLVTCDISGYGAAGPYRDMKAYDLLVQAESGLCSVTGTPEAPGR
                     VGVSVCDIAAGLNAFAGILEALYARERTGAGAAIEVSLFDGMAEWMCVPYLYQVHRGR
                     APARTGLTHPLVAPYGAFAAKDGKQVLISIQNEREWADLCAEVLDKPDLAADPRFSSP
                     ERRVANRAVLDALVAACFAHWPRDELMARLRAARIACGALNDVADLARHPQLRTVRYD
                     APSGPVEVIAPPVIHDGGGPAIRPVPRLGAHDAALRKEFAP"
     gene            complement(1781..2959)
                     /locus_tag="VGB88_06655"
     CDS             complement(1781..2959)
                     /locus_tag="VGB88_06655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9647160.1"
                     /translation="MADTEPTVPIAETFPEIRAGVRALCEKFPGSYWQGLDRARAYPT
                     EFVGALTAAGYLAALIPESYGGSGLPLAAAGAILEEVHRSGGNGAACHAQMYTMGTVL
                     RHGSEDQKRRYLPDIARGALRLQAFAVTEPTTGSDTTRLRTTAVREGNGYRVNGQKVW
                     TSRAEHSDLMVLLARTTPLDEAASRRAGLSVFLVDMREAVGNGLTIRPIRTMINHATT
                     ELFFDDLHIPADSLVGAEGKGFDYILDGMNAERILIASESLGDARFFIEKASAYAKTR
                     EVFGAPIGRNQGIQFPIARAFAATEAAELMARKAAALFDAGRACGPEANMAKLLASEA
                     AWAAADCCLQTHGGFGFAEEYDIERKWREARLYQVAPISTNLILSYLGQHVLGLPRSY
                     "
     gene            complement(3116..3403)
                     /locus_tag="VGB88_06660"
     CDS             complement(3116..3403)
                     /locus_tag="VGB88_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647161.1"
                     /translation="MEIDTSAAAFSGSAPYQPQLARQGESAHREQRDAVERATANPVA
                     AGDDDPARLRADAPEGRLREGDGDRPPVARSDASPPAERDTAHGRTVDLSV"
     gene            3686..>4681
                     /locus_tag="VGB88_06665"
     CDS             3686..>4681
                     /locus_tag="VGB88_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647162.1"
                     /translation="MDIAVSEPVAPARPAAPRAANKGAADAGFDDLLTAAQGATDAPA
                     DTPTPTTEAAAAPQATVPAAPAPDSPIPAAVAAALAGTLPAPAAEGAAAPPPAAADAG
                     GAQAVPQPPAIPFPAAPATAGDVPPPTDFPARTVPNPVAASAAAGIVAALQAGDGDQA
                     AAEAATTPLSPAAAQQAAVGRRQRPGQAATGQGTKSGQTGADGGKPGAQAAQARADGS
                     GPRLAATAGSEPSSPTRDIALRIAAQSTVPGPSPATVEAGSELGAFRDAVSSLSPDAS
                     GPATAAARTLAASAAAAVARAAPPPMPAIDQIVLHIHQAIEDELSHVRIQLHPKEL"
BASE COUNT          623 a         1682 c         1754 g          622 t
ORIGIN      
        1 gcggtgagcg ggccgtggac gatcaggccc tcatagccct cctcgccggt gacgtacggg
       61 tagtcgtagt ggatgcggtg gccgttgaag gtgagcgccg agtagcggaa cagcagcacc
      121 tcgtcgggcg tgatctcgcg ccgccagcgc gcctttgccg gcgcgccttt gcccggcggc
      181 agcgcggcac ccggcgccgg ctcggcgcga tagacgatgt cgtgccactc ctcgagcgcg
      241 aggccggccg cgcccgagta gcggtgatgg acggtgacga aggcgagcgc gccggcgcgc
      301 ccctccttgc gctcgatgcg ctcgatcacc gagtggcgcc gcgcccgctc gccgaccctg
      361 agcggctgat ggaagacgag ccggttgccg gcccacatgc gccgcggcag cgggatgggc
      421 ggcaggaagc cgccgcgcgc ctcgtgcccg tccgggccga gcgcgccggg gcgcggggtg
      481 tcggggaaat agagccagtg ccagccggga gggagagcat cgccttcggc gggcgcgcgc
      541 gcgtggtcga gggtcgccgc catggcgcgc gcctggaacg gcgcgatcag gtcctcggtc
      601 tcgcggctct tgccgatcca gctgtcaatg gactcggcgg ctcgggtcat ggcgcgaact
      661 ccttgcgtag cgcggcgtcg tgcgcgccga ggcgcggcac cgggcggatc gcgggcccgc
      721 cgccgtcgtg gatcaccggc ggcgcgatca cttcgaccgg gcccgagggc gcatcgtagc
      781 ggaccgtgcg gagctgcggg tggcgggcga ggtcggcgac gtcgttgagc gcgccgcagg
      841 cgatgcgcgc cgcgcgcagg cgcgccatca gctcgtcgcg cggccagtgc gcgaagcacg
      901 ccgcgaccag cgcatcgagc acggcgcggt tggcgacccg gcgctcgggg ctggagaaac
      961 gcggatcggc ggcgagatcg ggcttgtcca gtacctcggc gcagagatcc gcccactcgc
     1021 gctcgttctg gatcgagatc agcacctgct tgccgtcctt ggcggcgaag gcgccgtaag
     1081 gcgcgaccag gggatgggtg agcccggtgc gcgcgggcgc ccggccgcga tggacctggt
     1141 agaggtaggg gacgcacatc cactcggcca tgccgtcgaa cagcgacacc tcgatggcgg
     1201 cgcctgcgcc ggtgcgctcg cgcgcgtaca acgcctccag gatgccggcg aaggcgttga
     1261 gcccggcggc gatgtcgcac accgacacgc ccacccgtcc cggcgcctcg ggggtgccgg
     1321 tgaccgaaca cagccccgac tcggcctgga ccagcaggtc gtaggccttc atgtcccggt
     1381 acggcccggc cgcgccgtag cccgagatgt cgcaggtgac gaggcgcgga tgccgcgcgc
     1441 gcatctcggc ggtgccgatt cccgcgcgcg ccgccgcgcc cggtgccagg ttctggatgt
     1501 agacgtcagc cttggagatt attcgtttaa tcagaaatat atcttcctga ttcttgatat
     1561 cgaggatgag agattccttg ccccggttga gccacacgaa ataggcgctg tcgccgttaa
     1621 ccacgctgtc gtaagcgcgg gcgaagtcgc cctcggcacg ctcgaccttg atcacccgcg
     1681 cgccggcgtc ggcgagccgg gccgagcaca agggcgcggc caccgcctgc tcaagggtga
     1741 ccaccagaag gccgtcgagg gagcccccgg ccatgcccgg tcaataggag cgcggcaggc
     1801 cgagcacgtg ctggcccagg taggacagga tcaggttggt cgagatcggc gccacctgat
     1861 agaggcgcgc ctcgcgccac ttgcgctcga tgtcgtactc ctcggcgaag ccgaacccgc
     1921 catgggtctg caggcagcag tcggccgcgg cccaggccgc ctcggaggcg agcagcttgg
     1981 ccatgttggc ctcgggcccg catgcgcgcc cggcgtcgaa cagggcggcc gccttacgcg
     2041 ccatcagctc ggccgcctcg gtcgccgcaa acgcgcgcgc gatcgggaac tgaatgccct
     2101 ggttgcgccc gatcggggcg ccgaacacct cgcgcgtctt ggcgtaggcg ctcgcctttt
     2161 cgatgaagaa gcgcgcgtcg cccaaggatt ccgaggcgat caggatgcgc tcggcgttca
     2221 tgccgtcgag gatgtagtcg aagcccttgc cctccgcgcc gaccaggctg tcggcgggga
     2281 tatgcaggtc gtcgaagaac agctcggtgg tggcgtggtt gatcatggtc cggatcgggc
     2341 ggatggtgag cccgttgccg accgcttcgc gcatgtcgac caggaacacc gacaggcccg
     2401 cgcgccgcga cgcggcctca tcgagcgggg tggtgcgcgc gagcagcacc atcaggtcgg
     2461 agtgctcggc gcggctggtc cataccttct ggccgttgac ccggtagccg ttgccctcgc
     2521 gcaccgcggt ggtgcgcagc cgcgtggtgt cggagccggt ggtgggctcg gtgaccgcga
     2581 acgcctgcag gcggagcgcg ccgcgcgcga tgtcgggcag gtagcgccgc ttctggtcct
     2641 ccgagccgtg ccggagcacg gtgcccatgg tatacatctg ggcgtggcag gcggcgccgt
     2701 tgccgcccga gcggtggacc tcctcgagga tcgcgccggc ggcggcgagc ggcaggccgc
     2761 tgccgccgta ggactcaggg atcagggcgg cgagatagcc ggccgcggtg agcgcgccga
     2821 cgaactcggt gggataggcg cgggcgcggt cgagcccctg ccagtagctg cccgggaact
     2881 tctcgcacag ggcgcgcacg ccggcgcgga tctcggggaa ggtctccgcg atcgggacgg
     2941 tcggctcggt gtcggccatg ctgggcgggt ccccggtctc ggcgcggtgc cgcgcgcgcc
     3001 ggcggccaga tatagacgcg ccgctgcgcg gtcacaagcc gggccgctgg gccgacaaga
     3061 aaggggccca agggcccctc gccgtccggt gcgtgctatg tcggatacgg accggtcaga
     3121 ccgacaggtc tacggtccgg ccgtgcgcgg tgtcgcgctc cgccggtggc gaggcatcag
     3181 atcgggcgac gggcgggcgg tcgccgtcgc cctcgcgcag ccgcccctcg ggcgcatcgg
     3241 cgcgcagccg cgccggatcg tcgtcgccgg ccgcgaccgg gttggcggtc gcccgttcga
     3301 ccgcatcgcg ctgctcgcgg tgcgcgctct cgccctgccg cgccagctga ggctggtacg
     3361 gggcggagcc cgagaacgcg gctgccgaag tgtcgatctc catgatgcgg tcctccggcc
     3421 gtggtcgccc acggcgtcgt ccctactgga attagggtgg cgtcggcggc ggcgttgtga
     3481 ccgccctcac aaactcggcg gcggcaattt ctgccgccgg ctgcgggcag ttgattccgc
     3541 tgccgccggg cgggcgggcg cgaggacggc ggcgggcctt caccttttgt caacgattcc
     3601 aggcatccgc gctgctggcc cgatccttgc gagagcgcgc cccgcggcag tgcgtccgca
     3661 gcgtgacgac tctatggctc aggcattgga catcgccgtc tccgaaccgg tcgcgcctgc
     3721 ccgtccggcg gcgccgaggg ctgcgaacaa gggggcggca gacgcgggct tcgacgacct
     3781 gctgacggcg gcgcagggcg ccacggacgc gccggccgat acaccgacgc cgaccaccga
     3841 ggccgcggct gctccccagg ccaccgtgcc cgccgcgcct gcgcctgact ctccgattcc
     3901 ggcggccgtg gcggcggcgc tcgccggcac gcttcctgcg ccggcggctg agggcgcggc
     3961 ggcgccgcca ccggcggccg ccgacgcggg cggcgcgcaa gccgtgccgc agccgcctgc
     4021 catcccattc ccagccgcac cggccaccgc aggcgacgtt ccgccaccga ccgattttcc
     4081 ggcccggacc gtgccgaatc ccgtcgccgc ctcggctgcc gctggcatcg tggcggcgct
     4141 gcaagcgggc gacggcgacc aggcggcggc cgaggccgcg accacgccac tatcgccggc
     4201 ggcggcccaa caagcggccg tgggacgccg acagcggccg ggccaagccg ccaccggcca
     4261 gggcaccaag agcgggcaga ccggggccga cggcggcaag cccggcgcac aggctgcgca
     4321 ggcacgagcc gacggctcag ggcctcgcct ggccgccacc gccgggtccg aaccgtcgag
     4381 cccgacgcgt gacatcgcgc tgcgcatcgc ggctcagtcc accgtccccg ggccatcgcc
     4441 cgcgaccgtc gaggcgggct ccgagctcgg cgcgttccgc gacgcggtgt cgtcgctatc
     4501 gcccgatgcg tccggccccg cgaccgcggc cgcgcgcacg ctggcggcga gcgccgccgc
     4561 agccgttgcc cgcgccgcgc cgccgccgat gccggccatc gaccagatcg tgctgcacat
     4621 ccaccaggcg atcgaggacg agctcagcca cgtccgcatc cagcttcacc cgaaggagct
     4681 c
//
LOCUS       DASWHU010000180         7517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2679238, whole genome shotgun sequence.
ACCESSION   DASWHU010000180 DASWHU010000000
VERSION     DASWHU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679238"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VGB88_06670"
     CDS             complement(<1..453)
                     /locus_tag="VGB88_06670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9647163.1"
                     /translation="MLPAVLQVLPDMGAGGVAQGVVEVAAALSRAGFRALVASAGGPG
                     VAALEAAGAVHIALPLATKSPLLILRNAARLAALIRAREVAIVHARSRAPAWSAALAA
                     RRAGAHFVTTVHGTYGHSGALKRRYNAVMAAGEVVIAISEHISRHLTAV"
     gene            554..1339
                     /locus_tag="VGB88_06675"
     CDS             554..1339
                     /locus_tag="VGB88_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647164.1"
                     /translation="MTAPAPGRLDRPDGNVLAYHRSAGDSPAVVFCGGFMSDMTGTKA
                     CALESFCAERCQAYVRFDYLGHGASSGAFADGTIGRRAEDAVAVIDALSEGPVVLVGS
                     SMGGWIMLLAALARRARVQALVGVAAAPDFTRRMVEDELTEAQRAALARDGRIEIPSD
                     YGEAPYLITRALIDDGARHALLDGGIALTCPVRLLHGLKDAAVPWQTSLRLARALASA
                     DVTVTLVKDGDHRLSEPADLARLCAAVAEVSQPPARIARRPSR"
     gene            complement(1300..2178)
                     /locus_tag="VGB88_06680"
     CDS             complement(1300..2178)
                     /locus_tag="VGB88_06680"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647165.1"
                     /translation="MTAPRLFCFGMGYAATALARSLIAQGWRVAGTSRSAEGVGAIAA
                     LGAEGVRFAPGQPLADAHAALERTTHLLSSVPPGEAGDPVLAAHGGDVAHIAGLAWVG
                     YLSATSVYGDRGGGLVDETAAVAPSSARGRRRAGAEQDWLGLGRAHGLAVHVFRLAGI
                     YGPGRSALDQVRAGRARRIDQPGHSFARIHVEDIVTALCASMARPRAGAIYNLCDDAP
                     APSAEVVAYACELLGAAPPPLVPFAEAARSMSAMQREFWADNRRIDNRLMHEELGVVL
                     RHPSYREGLRAILAGG"
     gene            2256..2987
                     /locus_tag="VGB88_06685"
     CDS             2256..2987
                     /locus_tag="VGB88_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647166.1"
                     /translation="MTERRAWALVALALLPAAAGAAEGRYAACVALAESAPAGALETA
                     ERWLAEGGDTDAARHCRALALVGVGRAGDAAAELTRLADGAEAPPAVRASLYEQAGNA
                     WLLGGRADLAKAAFDRALALAPGAVEAHIDRARAEAALGDWWAAVEDLGRALDAEPAR
                     DDALALRASAWRRLEAWELAGDDVARALAVNPANADALLERAILALHAEQIDAARVDL
                     GRVLMLAPGGVAASLARDYLAELDR"
     gene            complement(2963..4114)
                     /gene="queG"
                     /locus_tag="VGB88_06690"
     CDS             complement(2963..4114)
                     /gene="queG"
                     /locus_tag="VGB88_06690"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9647167.1"
                     /translation="MPTSTSEAARAAIRARALGLGFDAVGFAAPGAIGAAGARLAEFV
                     GRGWHGDMGWLADTLARRADPHALWPEARAAVMVGLNYGPAGDALAPLARPDRATISV
                     YARNRDYHSLIKGRLKQLAGWMAGRFGAPVKVFVDTAPLLEKPLAEAAGLGWQGKHTN
                     LVSRELGSWLFLGAVLTALDLAPDAAESDHCGACRRCLDVCPTGAFPAPYQLDARRCI
                     SYLTIEHAGHIPRDLRAAMGNRIYGCDDCLAVCPWNKFARATAEAKLRERADLAAPRL
                     ADLARLDDAAFRTLFAGSPIKRIGRDRFLRNVLIAIGNSGEAALAPAAERRLADPSAL
                     VRAAAVWAVSRLVPAARFAELRARHLPAEADPDVAAEWRAPVQRSSSAR"
     gene            complement(4095..4763)
                     /locus_tag="VGB88_06695"
     CDS             complement(4095..4763)
                     /locus_tag="VGB88_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9647168.1"
                     /translation="MRTLFHLPLSAPSRAVRLALGEKGLAFALVPEPVWERRAEFLAL
                     NAAGEVPVLVEDGGPVACGGGVILEYLDEAYPERPLIGPGAAARAETRRLVDWFARKC
                     AAEVTEPLLHEKLYKRYLRLGYPDTEAIRAAQARLAEHLSYIEHLAERQRWLAGDELS
                     RADLVAAAELSVIDYLGDIPWPRFAAARDWYAKIKSRPAFRPLLEDYVPGTTPAAHYA
                     DLDF"
     gene            complement(4875..>7517)
                     /locus_tag="VGB88_06700"
     CDS             complement(4875..>7517)
                     /locus_tag="VGB88_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9647169.1"
                     /translation="AIDPPSLRELNSWPWPRDYHATLIDSLVAAGARTVALDIDFSSP
                     SDPVADARLEESLQAAAGRVVLPVFKEVHRGADGTEVILNEPLPRFRQHVRVGSVSVQ
                     PSRDGLVRELNTREVWADAAPPSFPALLAGLGDAPFDRFTLDFGIELGDVLELSYADV
                     LAGRFDPEVVRDKVVLVGATALGLQDFLPVPLYGVMSGVRVQALGYETLVGGRALHRL
                     GMLPIGVILALVALLVCARFAAWPWRRGLAVLIGLSATATAASLAVQAIVPVIVDVTP
                     VMLMSGLSYVAGLIIRVDAQAVRLVWQTIRLERTSALTRGIVEQSFDGIVTVGANGSI
                     ETANAAAESMFGYAAGGLGGRRLGELLCDPHPDSGDREAFLQSLHGYREVSARRRNGS
                     TFPAEIAVSDMEFEDDRMRIVIVRDVTEREAQRQRLEHQALHDDLTGLPNRRLLHERM
                     AYSLEIAKREGRQLALLLLDLDRFKEVNDTLGHPVGDALLHEVARRLGAVVRKTDTVA
                     RIGGDEFAILLHAVADQAKAGDIAQLVCRALERPFSFDGLSMDLHASVGIAIYPDHAD
                     EPARLMQRADVAMYLAKRLNLTFAFYDQDKDLNSVRNLTLTGELRRAIGSSQMCLHYQ
                     PKLDLRTGKMRGVEALARWRHPNLGDISAEEFIAHAERTGLIQPITEWVLDTAMAQLA
                     EWQRAGRDIAVAVNLSTRSLHDTSFPRLVASRIEAHRVDPSRLTFEITESALMLEPEC
                     AFEVIDRLHEIGVRLSVDDFGTGYSSLRYLQRMRLDEMKIDKSFVLGMVDNEGDALIV
                     RSTTDLAHNLGLTVVAEGVETDAHVAMLERLRCDLAQGYFFARPMPADALAAWLAQFQ
                     PAAARAKPAEQLAGAARAQAVAMA"
BASE COUNT         1031 a         2741 c         2798 g          947 t
ORIGIN      
        1 gacggcggtg aggtggcggg agatgtgctc ggagatcgcg atcaccacct cgcccgccgc
       61 catcaccgcg ttgtagcgcc gcttgagagc gcccgaatgg ccgtaggtgc cgtggacggt
      121 ggtgacgaag tgcgcgcccg cccgccgcgc cgccagcgcc gcgctccacg ccggcgcgcg
      181 gctgcgggcg tggacgatgg cgacctcgcg cgcgcgaatc agcgcggcca gtcgcgccgc
      241 gttgcgcagg atcaggagcg ggctcttggt ggcgagcggc aaggcgatgt gcaccgcgcc
      301 ggccgcttcc agcgcggcca cgccgggccc gccggccgag gcaaccagcg cccggaagcc
      361 ggcgcgggaa agcgcggcgg cgacctcgac cacgccctgg gcgaccccgc ccgcccccat
      421 gtccggcagc acctgaagta ccgctgggag caccgccggg gctcgcgccg cggttccggc
      481 cgggcgcacg gcggtgctag tctcgggcgc gtccaccatg cccgcttcat agcccaacaa
      541 cccgcgccca gccatgaccg cgcccgcacc cggccggctc gaccggcccg acggcaacgt
      601 cctcgcctat caccgcagcg ccggcgattc gcccgcggtg gtgttctgcg gcggcttcat
      661 gtcggacatg accggcacca aggcgtgcgc cctcgagagc ttctgcgccg agcgctgcca
      721 ggcctatgtg cgcttcgact atctcggcca cggcgcctcg tcgggcgcgt tcgccgacgg
      781 caccatcggc cgaagggcgg aggacgcggt ggcggtgatc gatgcgctca gcgaggggcc
      841 ggtggtgctg gtcggatcga gcatgggcgg ctggatcatg ctgctcgcgg cgctcgcccg
      901 gcgcgcgcgg gtgcaggcac tggtcggcgt cgcggcggcg cccgacttca cccggcgcat
      961 ggtcgaggac gagctcaccg aagcccagcg cgcggcgctc gcgcgcgacg ggcgtatcga
     1021 gatcccgtcg gactacggcg aggcgccgta cctgatcacc cgggcgctga tcgacgacgg
     1081 cgctcgccac gccctcctcg acggcgggat cgcgctcact tgcccggtgc ggctgctgca
     1141 cgggctcaag gacgccgcgg tgccgtggca aacctcgctt cgcctcgcgc gcgcgctcgc
     1201 ctcggccgac gtcaccgtca ccctggtcaa ggacggcgac caccgcctgt ccgagcccgc
     1261 cgacctcgcc cgcctgtgcg ccgcggtcgc cgaggtttct cagccgccgg cgaggatcgc
     1321 gcgcaggccc tcgcggtagc tcgggtggcg caagacgacg ccgagctcct cgtgcatgag
     1381 gcggttgtcg atgcgccggt tgtcggccca gaactcgcgc tgcatcgccg acatcgagcg
     1441 tgcggcctcg gcgaacggca ccagcggcgg cggtgcggcg cccaaaagct cgcaggcata
     1501 ggcgaccacc tcggcgctcg gcgccggcgc gtcgtcgcac aggttgtaga tcgcgccggc
     1561 acgcgggcgc gccatcgagg cgcagagggc ggtaacgatg tcctcgacat ggatgcgcgc
     1621 gaagctgtgc cccggctggt cgatccggcg cgcgcggccg gcgcgcacct ggtcgagggc
     1681 gctgcgcccc ggcccgtaga tgccggcgag gcggaacacg tgcaccgcca gcccgtgggc
     1741 gcgcccgagc ccgagccagt cctgctcggc gcccgcccgg cgccgcccgc gcgcgctcga
     1801 cggcgcgacc gccgccgttt cgtcgaccag gccgccgccg cggtcgccgt agacgctggt
     1861 cgccgacagg tagccgaccc aggcgaggcc ggcgatgtgg gcgacgtcgc cgccgtgggc
     1921 ggcgagcacc ggatcgcccg cctcgcccgg cggcaccgag ctcagcaggt gcgtcgtccg
     1981 ctcgagcgcg gcgtgcgcat ccgcaagcgg ctggccgggc gcgaagcgga ctccctcggc
     2041 gccgagggcc gcgatcgcgc ccacgccctc ggcactgcgg ctggtgccgg cgacgcgcca
     2101 gccctgcgcg atcaggctcc gcgccagcgc ggtcgcggca tagcccatgc cgaagcagaa
     2161 caggcgcggc gcggtcacga atcgccctcg ttgccggttg cgctgaacgg tgcggcgggt
     2221 tggtatacgg gcgatgccga gccattgcca agccgatgac tgagcggcgc gcctgggcgc
     2281 tggtcgcgct ggcgctgctg ccggcggcgg ccggcgccgc cgagggccgc tacgccgcct
     2341 gtgtcgcgct cgccgagagc gcgcccgccg gcgcgcttga gaccgccgag cgctggctcg
     2401 ccgagggcgg cgatacggat gcggcgcgcc actgccgcgc gctcgccctg gtcggtgtcg
     2461 ggcgcgccgg cgacgccgcg gccgagctga cccggcttgc cgacggcgcc gaggctccgc
     2521 ccgcggtgcg cgcctcgctc tacgagcagg cgggcaacgc gtggctgctg ggcgggcgcg
     2581 ccgaccttgc caaggcggcg ttcgaccggg cgctggcgct cgccccgggc gcggtcgagg
     2641 cgcacatcga ccgggcgcgg gccgaggcgg cgctcggcga ctggtgggcg gcggtcgagg
     2701 acctcggccg cgcgctcgac gccgagcccg cgcgcgacga cgcgctggcg ctgcgcgcct
     2761 cggcctggcg ccggctggag gcgtgggagc tggcgggcga cgacgtcgcc cgcgcgctcg
     2821 ccgtcaaccc ggccaacgcc gatgcgctgc tcgagcgcgc gatcctcgcc ctccacgccg
     2881 aacagatcga tgccgcgcgt gtcgacctcg gccgggtgct gatgctggcg ccgggcgggg
     2941 tggccgcgag cctcgcccgc gattacctgg ccgaactcga ccgctgaacc ggcgcccgcc
     3001 actcggcggc gacgtcgggg tcggcctcgg cgggcagatg gcgggcgcgc aattcggcga
     3061 agcgcgccgc cggcaccagc cgcgagaccg cccacaccgc ggccgcccgc accagcgccg
     3121 acggatcggc gagccggcgc tccgccgccg gcgcgagggc ggcctcgccg ctgttgccga
     3181 tcgcgatcag cacgttgcgc aggaagcggt cgcggccgat gcgcttgatc ggcgagccgg
     3241 cgaacagcgt gcggaaggcg gcatcgtcga ggcgggcgag gtcggcgagg cgcggggccg
     3301 cgagatcggc gcgctcgcgc agcttggcct cggcggtggc gcgcgcgaac ttgttccacg
     3361 ggcacaccgc caggcagtcg tcgcagccat agatgcggtt gcccatcgcg gcgcgcaggt
     3421 cgcgcgggat gtggccggcg tgctcgatgg tcaggtacga gatgcagcgc cgggcgtcga
     3481 gctggtaggg cgccgggaag gcgccggtcg ggcacacgtc gaggcagcgt cggcacgcgc
     3541 cgcagtggtc ggactcggcc gcgtcggggg cgaggtcgag ggcggtcagc accgcgccga
     3601 ggaacagcca cgagccgagc tcgcgcgaga ccaggttggt gtgcttgccc tgccagccga
     3661 gcccggcggc ctcggccagc ggcttctcga gcaagggtgc cgtgtcgacg aacaccttga
     3721 ccggcgcgcc gaagcggccg gccatccagc cggcgagctg cttgagccgg cccttgatca
     3781 ggctgtgata gtcgcggttg cgggcgtaga ccgagatcgt cgcccggtcg ggccgggcga
     3841 gcggcgcgag cgcatcgccc gccgggccgt agttgagccc gaccatgacc gcggcgcgcg
     3901 cctcgggcca cagcgcgtgc gggtcggccc gccgcgcaag ggtgtcggcg agccagccca
     3961 tgtcgccgtg ccagccgcgg ccgacgaact cggcgagtcg cgcgccggcg gcgccgatcg
     4021 caccgggcgc ggcgaagccg acagcgtcga agccgaggcc gagcgcccgc gcacggatcg
     4081 ccgcgcgcgc cgcttcagaa gtcgaggtcg gcataatgcg ccgccggcgt cgtgcccgga
     4141 acgtagtcct cgagcagcgg ccggaacgcc gggcgcgact tgatcttggc gtaccagtcg
     4201 cgtgccgcgg cgaagcgcgg ccaggggatg tcgcccagat agtcgatcac cgacagctcc
     4261 gccgcggcga ccaggtcggc gcggctcagc tcatcgccgg cgagccaccg ctgccgctcg
     4321 gccaggtgct cgatatagga caggtgctcc gccagccgcg cctgggcggc gcggatcgcc
     4381 tcggtgtcgg gatagccgag gcgcaggtag cgcttgtaaa gcttctcgtg cagcaggggc
     4441 tcggtcacct ccgccgcgca tttgcgcgcg aaccagtcga ccagccggcg cgtctcggcc
     4501 cgcgctgcgg cgcccggacc gatcagcggg cgctccggat aggcctcgtc gagatattcg
     4561 aggatgacgc cgccgccgca cgccaccggc ccgccgtcct cgaccagcac cggcacctcg
     4621 cccgccgcat tcagcgccag gaactcggcc cggcgctccc acaccggctc gggcacgagc
     4681 gcgaacgcga gccccttctc gcccagcgcc aggcgcacgg cgcggctcgg cgccgacagc
     4741 ggcaggtgga acaatgtgcg catgcgctcg gcccggcgct cgagggtcac tcgaggcatt
     4801 gtgcggcgtg cggcaccggc acacaacgtg tcccgcggac ggccccgcag ccgagcgtga
     4861 cgagcggcgc gacctcaagc catcgcgacg gcctgggccc ttgccgcgcc cgccagctgc
     4921 tccgcgggct tcgcccgcgc cgcggccggc tggaattgcg cgagccacgc cgccaacgcg
     4981 tcggccggca tcgggcgggc gaagaaataa ccttgggcga ggtcgcagcg cagccgctcg
     5041 agcatcgcga catgcgcgtc cgtctcgaca ccttcggcga ccaccgtcag gccgagattg
     5101 tgggcgaggt cggtggtcga gcgcacgatc agggcgtcgc cttcgttgtc gaccatgccg
     5161 agcacgaacg acttgtcgat cttcatttcg tcgagccgca tgcgctgcag atagcgcagc
     5221 gacgagtagc cggtgccgaa gtcgtcgacc gacagccgga cgccgatctc gtgcagccgg
     5281 tcgatcacct cgaacgcgca ttcgggctcg agcatcagcg cgctctcggt tatctcgaag
     5341 gtcaggcgcg acggatcgac gcggtgcgcc tcgatgcggc tcgcgaccag gcgcggaaag
     5401 ctggtgtcgt gcagcgaccg cgtcgacagg ttgacggcga cggcgatgtc ccggccggcg
     5461 cgctgccatt cggccaactg cgccatggcg gtatcgagca cccactcggt gatcggctgg
     5521 atcagtccgg tgcgctcggc atgggcgatg aactcctcgg ccgatatgtc gccgagattt
     5581 ggatgacgcc accgagccag ggcttcgaca ccgcgcatct tgccggtgcg caggtcgagc
     5641 ttcggctggt agtggaggca catctggctg ctgccgatgg cgcggcgcag ctcgccggtg
     5701 agcgtcaggt tgcggacgct gttgaggtcc ttgtcctggt cgtagaaggc gaaggtcagg
     5761 ttcaggcgct tcgccaggta catggcgacg tcggcgcgct gcatcaggcg cgccggctcg
     5821 tccgcgtggt ccggatagat cgcgatgccg acgctggcgt gcaggtccat cgacaggccg
     5881 tcgaaagaga acggccgttc cagcgcgcgg caaaccagct gcgcaatgtc gccggccttc
     5941 gcctggtcgg cgaccgcatg caagaggatc gcgaactcgt cgccgccgat gcgggcgacg
     6001 gtgtcggtct tgcgcaccac cgcgccgaga cggcgcgcca cctcgtgcag cagggcgtcg
     6061 cccaccgggt ggccgagggt atcgttgacc tccttgaacc ggtcgaggtc aagcagcagc
     6121 agcgccagtt gccggccctc gcgcttggcg atctcgagcg aataggccat gcgctcgtga
     6181 agcagccggc ggttcggcag cccggtcagg tcgtcatgca gggcctgatg ctcgagccgc
     6241 tgcctttgcg cctcgcgctc ggtgacgtcg cgcacgatca cgatgcgcat gcggtcgtcc
     6301 tcgaactcca tgtcgctgac cgcgatctcg gccgggaagg tggagccgtt ccggcgccgc
     6361 gccgacactt cccgatagcc gtgcaggctt tgcaggaagg cctctcgatc cccgctgtcg
     6421 ggatgcggat cgcacaggag ctcgccgagc cggcggccgc caaggccgcc cgcggcgtag
     6481 ccgaacatgc tttcggccgc cgcgttcgcg gtctcgatcg agccgttcgc gccgaccgtg
     6541 acgatgccgt cgaagctctg ctcgacgatg ccgcgcgtca acgcgctggt gcgctcgagc
     6601 cggatggtct gccagaccag gcggacggcc tgggcgtcga cgcggatgat cagcccggcg
     6661 acgtaggaca ggccggacat cagcatcacc ggggtcacgt cgacgatcac cggcacgatc
     6721 gcctggaccg cgagcgaggc ggcggtcgcg gtggcggaca ggccgatcag gaccgcgaga
     6781 ccgcgccgcc acggccacgc ggcgaaccgc gcgcagacca gcagcgccac cagtgccagg
     6841 atgacgccga tcggcaacat gccgagccga tgcagcgccc ggccgccgac cagggtttcg
     6901 tagcccaggg cctggacgcg caccccgctc atgacgccgt acaaggggac cggcaggaag
     6961 tcctgcaggc cgagcgcggt ggcgccgacc aggacgacct tgtcgcgaac cacctccggg
     7021 tcgaaccggc cggcgagcac gtccgcgtag gagagctcga ggacatcgcc gagctcgatg
     7081 ccgaaatcga gcgtgaagcg gtcgaacggc gcgtccccca gcccggcgag cagcgccgga
     7141 aacgacggcg gggcggcgtc ggcccagacc tcgcgcgtgt tcagctcgcg gaccaggccg
     7201 tcgcgcgacg gctgcacgct gaccgagccg acgcgaacat gctgcctgaa gcggggcagg
     7261 ggctcgttga ggatcacctc ggtgccgtca gcgccccggt gaacctcctt gaacaccggc
     7321 agcaccaccc gcccggccgc agcctgcagc gactcctcga gccgcgcatc ggccaccgga
     7381 tcggacggcg agctgaaatc gatatccagc gccaccgtcc tggcgccggc cgcgaccagg
     7441 ctgtcgatca gggtggcatg gtagtcccgc ggccacggcc agctattgag ctcgcgcagg
     7501 ctgggcggat cgatcgc
//
LOCUS       DASWHU010000181         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_825236, whole genome shotgun sequence.
ACCESSION   DASWHU010000181 DASWHU010000000
VERSION     DASWHU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825236"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1684)
                     /locus_tag="VGB88_06705"
     CDS             complement(302..1684)
                     /locus_tag="VGB88_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9647170.1"
                     /translation="MTGRPAEAGRVLIVDDEATFARAVATRLARERYDRRIAGSLADA
                     RPHLAAGADWTPDLVLLDMRLPDGTGLELLDQLAPTSDAPAVIAVTAFGDIDNAVEAM
                     KRGARDYLRKPVDLDDLVHAIERTLAAARLQTRLSYSRERDSHAVDPAELIGKSPAIE
                     AVRRELATIAALAGDDGAPPPTVLLAGETGSGKDIAARLIHSLGRRADQPFVRIDCAT
                     LSLEAAERELFGESDAGAAPGLIEAAEGGSVFLDEIAELAPAVQARLLSVIERRRLRR
                     PGGGREVGVAARFIAGTNRDLTAQVKAGAFRADLYYRLNVLTVRLPPLRERAGDATLL
                     ARHFAAAVARRYGRPAPAFAEPALAMLERYPWPGNVRELGHLIERAVLLDSDGEIGPR
                     DLAMPGPVVAAGDGSDPYAALKGMTVEGAERWLMARALERAGGNVSAAARALGLTRMA
                     LRYRMEKYGL"
     gene            complement(1681..3150)
                     /locus_tag="VGB88_06710"
     CDS             complement(1681..3150)
                     /locus_tag="VGB88_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647171.1"
                     /translation="MSTTRSLRTLQLVYGLALTILIVVAGAAGIVGLVLAERWSGEAR
                     RVERLAQLAQVVRGDVYRQAKEVFDFHFLADPQASAQYSSYAGRIDASFAELAGLAEG
                     AGEQAAIAELAAAYAGARAQADAIMARPSGGFSEAEKLDLFDTDYEAGSLARVEAALD
                     HAEDAFLGARAAVERRLKGLTRLAVAVVLVPIAAAAGLLLVARAFVQRAFVAPLAEVL
                     RATAAFGAGDFAGRLDERGAAEMVALGRAINRMADDVARSRAELVRAEKQAALGALVP
                     VIAHNIRNPLASIRASAQVLDTGAADAETRDGLRGIIAAVDRLSGWLTALLSYLNPQP
                     LHRRDGTLAACADEALALLGPRLEAKRLEVARRGWDAGATVRLDRHLVEQAIYGLLAN
                     AVEASPDGGRLTVAAGAADGASWLSIEDHGPGLAFAPRQDAFPGPSTKALGSGLGIPF
                     AFKVCELHDGALKFAPAAGGGTAVTLSLPIAGDGPRKAA"
     gene            complement(3233..3538)
                     /locus_tag="VGB88_06715"
     CDS             complement(3233..3538)
                     /locus_tag="VGB88_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9647172.1"
                     /translation="MPGQCGRRLCAIAAAAALAAPAAAGELFGALPAGPGHDEVEITC
                     SACHSLAIVKQQRLNRTVWDQLLVWMVEEQGMPELDGDERALILDYLAAHFGPHVPR"
     gene            complement(3538..>4159)
                     /locus_tag="VGB88_06720"
     CDS             complement(3538..>4159)
                     /locus_tag="VGB88_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9647173.1"
                     /translation="GLPIAKAMQPNTLIAFEMNGEPIPMLHGAPLRVVAPGWPGSVSQ
                     KWLNRIWVRDRVHDGEKMTGMSYRVPAHSVAPGSEVPKEDMRIIEAMPVKSVITRPQA
                     GAAVAGATPLGARGHAWAGDRAVAAVDVSIDFGATWQRARLEPPVNAGAWQTWSADVA
                     FPQAGYYELWARATDDAGTMQPMVVPGWNPHGYLNNACHRIAVTVA"
BASE COUNT          558 a         1483 c         1551 g          567 t
ORIGIN      
        1 cgtcgtccgc gtcctgggcc agcgcgccgg cgccggcaat tcccatgacc gccgcggcgg
       61 cgagccactg cctcgcccgc ccggcgccga tcgagccctc cccgctcatt gtctcagccc
      121 cgtcgtagga taagttttcc tacgattaca gtctaataat gtctaatgtc aatgaaatta
      181 tggaagaaat gccgattaat agattgaatt catgcgttta tttctggttg aaatgcccca
      241 atatcggtta cctggggcaa gatttcggcg gacactggct gatttcagcc gatcgcgcgg
      301 gtcacaggcc gtatttttcc atgcgatagc gcagcgccat gcgggtgaga ccgagggcgc
      361 gcgcggccgc cgagacgttg ccgccggcac gctcgagcgc gcgcgccatc agccagcgct
      421 cggcgccctc gacggtcatg cccttgaggg ccgcataggg gtcggacccg tcgccggccg
      481 cgaccaccgg gccgggcatg gcgagatcgc gcgggccgat ctcgccgtcg ctgtcgagca
      541 acacggcgcg ctcgatcagg tggccgagct cgcgcacgtt gcccggccac ggatagcgct
      601 cgagcatggc gagcgcgggc tcggcgaacg ccggcgccgg ccggccgtag cggcgcgcga
      661 ccgcggcggc gaagtggcgg gcgaggaggg tcgcgtcgcc cgcgcgctcg cgcagcggcg
      721 gcagccgcac ggtgagcacg ttgagccgat aatagaggtc ggcgcggaag gcgcccgcct
      781 tgacctgcgc ggtcaggtcg cggttggtgc cggcgatgaa ccgcgccgca accccgacct
      841 cgcgcccgcc gccgggccgg cgcagccgcc ggcgctcgat caccgacagc agccgcgcct
      901 ggaccgcggg cgcgagctcg gcgatctcgt cgagaaacac cgagccccct tcggcggcct
      961 cgatcagccc cggcgcggcg cccgcgtcgg actcgccgaa cagctcgcgc tcggcggcct
     1021 caagcgacag cgtcgcgcag tcgatgcgca cgaacggctg gtcggcgcgc cgcccgaggc
     1081 tgtggatcag gcgcgcggcg atgtccttgc ccgagccggt ctcgccggcc agcagcacgg
     1141 tcggcggcgg cgcgccgtcg tcgccggcga gcgcggcgat ggtcgcgagc tcgcgcctga
     1201 ccgcctcgat cgccgggctc ttgccgatca gctcggcggg atcgacggcg tggctgtcgc
     1261 gctcgcggga atagctcagg cgggtctgca ggcgcgccgc cgcgagcgtc cgctcgatcg
     1321 cgtgcacaag gtcgtcgagg tcgaccggct tgcgcagata gtcgcgtgcg ccgcgcttca
     1381 tcgcctcgac cgcgttgtcg atgtcgccga aggcagtgac cgcgatcacc gccggcgcat
     1441 cgctcgtcgg tgcgagctgg tcgagcagct cgaggccggt gccgtcgggc aggcgcatgt
     1501 cgagcagcac caggtcaggt gtccagtcgg cgcccgcggc gaggtgcggg cgggcgtcgg
     1561 cgagcgagcc ggcgatgcgg cggtcgtagc gctcgcgcgc gagccgggtg gcgacggcgc
     1621 gggcgaaggt cgcctcgtcg tcgacgatca gcacccgccc tgcctctgcc ggccggccgg
     1681 tcatgcggcc ttgcgcgggc cgtcgccggc gatcggcagc gacaaggtga ctgcggtgcc
     1741 gccgccggcg gcgggcgcga atttgagcgc gccgtcgtgc agctcgcaca ccttgaacgc
     1801 gaacgggatg ccgaggccgc taccgagcgc cttggtgctg ggaccgggga aggcgtcctg
     1861 gcgcggcgcg aacgcgagcc cggggccgtg gtcctcgatg ctgagccagc tcgcgccgtc
     1921 ggcggcgccg gcggcgacgg tcaggcggcc gccgtcgggc gaggcctcga ccgcgttggc
     1981 gagcaggccg tagatcgcct gctcgaccag atgcctgtcc aggcgcacgg tcgcgcccgc
     2041 gtcccagccc cggcgtgcga cctcgagccg cttggcctcg agtcgggggc cgagcagcgc
     2101 cagggcctcg tcggcgcacg cggcgagggt gccgtcgcgg cggtgcagcg gctgcgggtt
     2161 caggtatgac agcagcgccg tcaaccagcc gctgagccgg tcgaccgcgg cgatgatgcc
     2221 gcgcaagccg tccctcgtct cggcgtcggc ggcgccggtg tcgagcacct gtgcggacgc
     2281 gcggatgctg gccagcggat tgcgaatgtt gtgcgcgatc accggcacca gcgcgccgag
     2341 cgcggcctgc ttctcggccc gcaccagctc ggcgcggctg cgcgcgacgt cgtcggccat
     2401 gcggttgatg gcgcgcccga gcgcgaccat ctcggcggcg ccgcgctcgt cgagccggcc
     2461 ggcgaagtcg ccggcgccga acgcggcggt ggcgcgcagc acctcggcca ggggcgcgac
     2521 gaaggcgcgc tggacgaacg cgcgcgcgac cagcaacagc ccggccgcgg cggcgatcgg
     2581 caccaggacg acggcgacgg cgaggcgggt gaggcctttc aggcgccgct cgaccgccgc
     2641 gcgggcgccg aggaaggcgt cctcggcgtg gtccagcgcg gcctcgaccc tggccaggct
     2701 gccggcctcg tagtcggtgt cgaacaggtc gagcttctcg gcctcggaga agccgccgct
     2761 cgggcgcgcc atgatcgcgt cggcctgggc gcgggcgccg gcataggccg ccgccagctc
     2821 ggcgatggcg gcctgctcgc cggcgccctc ggcgagccct gccagctcgg cgaagctcgc
     2881 gtcgatgcgc ccggcgtagc tcgaatattg cgcggaggct tgcggatcgg ccaggaagtg
     2941 gaagtcgaac acctcctttg cctgccggta gacgtcgccg cgcaccacct gggcgagctg
     3001 ggccaggcgc tcgacccgcc gtgcctcgcc gctccagcgt tcggccagca ccaggccgac
     3061 gatgccggcg gcgccggcga cgacgatcag gatcgtcaac gccaggccat agaccagctg
     3121 cagggtcctg agcgaacgcg tggtactcat gtcgccgcca tggttgccgg accagggtgg
     3181 ttgatttcag ccacccccgt caagcgcact gcggacggcg cggggtgcgg cgtcagcgcg
     3241 gcacgtgcgg cccgaaatgc gccgccaggt aatcgaggat cagggcgcgc tcgtcgccat
     3301 cgagctcggg catgccctgc tcctcgacca tccataccag cagctggtcc cacaccgtgc
     3361 ggttgaggcg ctgctgcttg acgatggcga gcgaatggca ggccgagcaa gtgatctcga
     3421 cctcgtcatg gccgggtccc gccggcaggg cgccgaacag ctcgccggcg gcggcgggcg
     3481 cggccagcgc cgcggcggcg gcgatggcgc aaagccggcg cccgcattgc ccgggcatca
     3541 ggcgacggtg accgcgatcc ggtggcaggc gttgttgaga tagccgtgcg ggttccatcc
     3601 cggaaccacc atcggctgca tggtgccggc gtcgtcggtc gcccgtgccc acagctcgta
     3661 gtagccggcc tggggaaagg cgacgtcggc ggaccatgtc tgccacgcgc cggcattgac
     3721 tggcggctcg agccgcgcgc gctgccaggt ggcgccgaag tcgatgctga catcgaccgc
     3781 ggccacggcg cgatcgccgg cccaggcgtg gccgcgcgcg ccgagcggcg ttgcgccggc
     3841 gaccgcggcg ccggcctggg gccgggtgat caccgatttc accggcatcg cctcgatgat
     3901 cctcatgtcc tccttcggca cctcgctccc cggcgccacc gaatgggcgg gaacgcgata
     3961 ggacatgccg gtcatcttct cgccgtcatg gacccggtcg cggacccaga tgcggttcag
     4021 ccacttctgc gacaccgagc ccggccagcc gggagcgacc acgcgcagcg gcgcgccgtg
     4081 cagcatcggg atcggctcgc cgttcatctc gaacgcgatc agcgtgttgg gctgcattgc
     4141 cttggcgatc ggcaggccg
//
LOCUS       DASWHU010000182         7478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_825826, whole genome shotgun sequence.
ACCESSION   DASWHU010000182 DASWHU010000000
VERSION     DASWHU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825826"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..672
                     /locus_tag="VGB88_06725"
     CDS             337..672
                     /locus_tag="VGB88_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647174.1"
                     /translation="MAKIDEGEWRKRLERISQMFADMVGHAEELSQHRCPYRDRHDRC
                     TAHFRCRSQLAQDAADKPALCTHDGSFDYRLAWESDPATYDRAKRRIADAKRGPRRAR
                     RRAGDTPGS"
     gene            669..2342
                     /locus_tag="VGB88_06730"
     CDS             669..2342
                     /locus_tag="VGB88_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX9647175.1"
                     /translation="MSTISHDRAARPVETGRTIFDYADELAVEVPTSCGRAGRCHECV
                     VEVSRGMAALSAPSAAESFLRGDWRLACQAVIERDDIDISFAPLRRRPRILEDSDGED
                     AVPELDPAVRRHGDAVLYDGQVIDRWRGALYGLAVDLGTTTVVADFVDLESGRSVHRI
                     GFENPQRFGGSDVMNRISYDTGPNRGELQQAAVTALNRELRRAAREHGIARQAVYEIV
                     IAGNTTMRDILFGLDVASVGQKPYKSLVENDWRAGRRATTALTCEAWSLGLRANRRAR
                     VYGLPLIASHVGGDTAADLVATDFAALAEHSVVMLVDMGTNTEVVVGHRGRLVAASCP
                     AGPAFEGGLVAYGMAAYDGAIERIELDPASGLFRYQTIADAPAQGLCGSGLIDLLAEL
                     RRHGRMTAKGVFADDRRKFLIEVVPERGITFSKADASALAQAKAANYCGQLIVLRTLG
                     LDPGAVERLFLAGGFATYVDTANAIDIGLLAPVPEAAVVKVGNAALRGAREVLLSLPK
                     RRRLEALVQRIEHVELETTPDFFDLFVDGCQFRPMPDVLTPPPQQRTAG"
     gene            2339..3388
                     /locus_tag="VGB88_06735"
     CDS             2339..3388
                     /locus_tag="VGB88_06735"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9647176.1"
                     /translation="MKLADGRTLIVIGENIHTTRVVLRKGRRFVDDGARQGVRYTGND
                     GAERLLPVPDWAKRGQDYQEGRVKHVMIAVRAAMAGAGAEAAQGMDYIARMVSDLEGA
                     GADYLDLNVDEISIKLADQKAAIAWLVRTVQSLTALPLSVDSSNLEVIEAGLAACDGA
                     RARPLLNSASLERIDALDLAAAAGAQVVVTAAGDSGMPDGVAQRLDNASRMVEAARAK
                     GFALSDIFIDPLLFPVSVDTVYGPHGLDAIRALRARFGPDIHITGGMSNVSFGIPARK
                     VINEVFLLLALEAGADSGIIDPVASPPQALLAIDRSSPSYKLAEDVLLDRDKHCRSFI
                     RAWRKGELAPLAAGA"
     gene            3406..4287
                     /locus_tag="VGB88_06740"
     CDS             3406..4287
                     /locus_tag="VGB88_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HEX9647177.1"
                     /translation="MTGLAVNLVLGFLGAGKTTLVARLLDHLKGRERVALVVNEFGAV
                     GIDGAVLEGRAVDTVELSSGCVCCTLKAPLQAALGELAGRGDIDRVIIEASGVAQAAD
                     LVAALAEDDLSLGPIIAVVDAARFAQLNAMLGPFYAGQVARADVVIVNKCDLAGAATL
                     SEVAEAVAALNPRARLAFAERCDVDPADVLAGAAAPAAGLADDHLHQAAETLVLAAPE
                     AARRAEVERFFAALPPGLWRAKGFMSIDGAPRLVQYACGELEIAPAPPRAQLHVVFVG
                     RALDRADLARRFAALGR"
     gene            4365..4982
                     /locus_tag="VGB88_06745"
     CDS             4365..4982
                     /locus_tag="VGB88_06745"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019298365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9647178.1"
                     /translation="MFYRPPAPHGLRQNPFNALVIPRPIGWISSLDTEGRANLAPYSF
                     FNAVAYTPPQVMFAATGLHEQGGFKDTVANIQATGEFVVNLATWPLREAMNACSAPAP
                     HDVDEFKLAGLTPAPAETVKPPRVAESPVHLECRHVRSLELLSANPESPNMVVFGEVV
                     GIHIDDTVIADGMVRYDRLDVVGRLGYRDYVRVNDIFAMERPGWP"
     gene            5046..6500
                     /locus_tag="VGB88_06750"
     CDS             5046..6500
                     /locus_tag="VGB88_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9647179.1"
                     /translation="MTTAARDDLFAGYDRDGHFCELFGRAGLPAPAHTSLLRERLASY
                     RLDTLKKRARDAERELFNLGITFTVYSEKDAVDRILPFDPLPRVLSADDWRTIESGVV
                     QRVTAINLFLHDIYHRQTILADGVVPRALVLGNVNYRPEMRGLDLPCGTYVHICGIDI
                     VRDAAGGFLVLEDNARTPSGVSYVVENRHLMLRTFPDLMTGIGIRYVSDYGRRLREAL
                     QELAPVGVAEPQVALLSPGAYNSAYFEHVFLAREMGVPLVEGRDLVVEDERVYMLTTE
                     GREPVDVIYRRIDDDFLDPECFRADSLLGVRGLMRACRAGRVALANAVGTGVADDKAI
                     YAYMPRVVRYYLDQDPILPNVETHICADADGLAYTLDHVNELVVKPVGASGGYGITVG
                     PLASRVELKACRKRIAERPEDYISQPMVELSVCPTLTDRGVEPRRVDLRPFAVTGRDS
                     WVLPGGLTRVALRRGSIVVNSSQGGGSKDTWVLA"
     gene            6517..>7478
                     /locus_tag="VGB88_06755"
     CDS             6517..>7478
                     /locus_tag="VGB88_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX9647180.1"
                     /translation="MGNSLLARFASNAFWMARYMERAENLARLLDVNETFARDSQGVQ
                     DWLPIVQLHIDDEPFFKLYAQADAPSVLEFYILERRNPNAIVSAVAVARENARSLRHL
                     ISTELWLHLNMFHNRLMAMRPADLALETVSELCRTVKEDCQLHAGIAEGTLYRDQVWS
                     FYRIGKTIERLDQTTRLLDIKYHRLGPSSDKAGELIDTAQWNALLRSAAGYQAFRRVY
                     PTGMRRENVIRFFLYDRAFPRSVGLCVDELGKELDGLGRAKRATGWPEVAMSHDALRA
                     LVRLDDAAAPVAEGDLHRHIDDIQLGLIRLTDALGRAFLGARPD"
BASE COUNT         1089 a         2737 c         2550 g         1102 t
ORIGIN      
        1 cgtctcgacc agccgatcga actcatgcca aggaacatgc ttcagaaccg aatgaaaaac
       61 gctattgtga tggcgcatgg cgtcgatcct cgcttcgtgt cccggaacct ctgctgaagt
      121 ccaggatcga gtagaatcaa cgccatgcac tccgtccaca aaaaccgaac cggacagccg
      181 tgggcccgac ccgcgcatcc acgtgcttcc gtccggaatt tcctcgtcgg aaggacgtgg
      241 atggccgtgt caagcacggc catgaccatt tgtgagaacc gaaacgtgtt gagacgaccc
      301 tcatcgctct cgatcgggtt ctgagtacac gcggacatgg ccaagatcga cgagggcgaa
      361 tggcgcaagc gcctcgagcg catctcgcag atgttcgccg acatggtcgg ccatgccgag
      421 gagctgtcgc agcaccgctg cccctatcgc gaccgccacg accgctgcac cgctcatttc
      481 cggtgccgta gtcagttggc gcaggacgcg gcggacaagc cggcgttgtg cacccacgac
      541 ggcagcttcg actaccggct cgcctgggag agcgatcccg cgacctatga ccgagccaag
      601 cggaggatcg cggacgcgaa gcgggggccg cggcgggcgc gccggcgggc gggcgatacg
      661 cccgggtcat gagcaccatc tcgcacgacc gcgcggcgcg cccggtcgag accggccgca
      721 ccatcttcga ttacgccgac gagctcgcgg tcgaggtgcc gacctcgtgc gggcgcgccg
      781 ggcgctgcca cgaatgcgtg gtcgaggtga gccgcggcat ggccgcgctg agcgcgccga
      841 gcgcggcgga atcgttcctg cgcggcgact ggcgcctcgc ctgccaggcg gtgatcgagc
      901 gcgacgacat cgacatttcg ttcgcgccct tgcggcgccg gccgcgcatc ctcgaggatt
      961 cggacggcga ggacgccgtg cccgagctcg accccgccgt gcgccggcac ggcgatgcgg
     1021 tgctctacga cggccaggtg atcgaccgct ggcgcggcgc gctctacggc ctcgccgtcg
     1081 acctcggcac gaccacggtg gtcgccgact tcgtcgacct cgagagcggc cgctcggtcc
     1141 accggatcgg cttcgagaac ccgcagcgct tcggcggcag cgacgtgatg aaccgcatct
     1201 cctacgacac cggccccaac cgcggcgagc tgcagcaggc ggcggtcacc gcgctcaacc
     1261 gcgagctgcg ccgggccgcg cgcgagcacg gcatcgcgcg ccaggcggtg tacgagatcg
     1321 tcatcgccgg caacaccacc atgcgcgaca tcctgttcgg cctcgacgtc gccagcgtcg
     1381 gccagaagcc gtacaagtcg ctggtcgaga atgactggcg cgcgggcagg cgcgctacca
     1441 ccgcgctcac ctgcgaggcg tggtcgctcg gcctgcgcgc caaccgccgg gcgcgcgtct
     1501 acggcctgcc gctgatcgcg agccacgtcg gcggcgacac cgcggccgac ctggtcgcga
     1561 ccgacttcgc cgccctcgcc gagcacagcg tggtgatgct ggtcgacatg ggcaccaaca
     1621 ccgaggtcgt ggtcggccat cgcgggcgcc tggtcgcggc ctcctgcccg gcaggccccg
     1681 cgttcgaggg cggcctggtc gcctacggca tggccgctta tgacggcgcc atcgagcgca
     1741 tcgagctcga ccccgcctca gggctctttc gctaccagac gattgccgac gcgccggcgc
     1801 aggggctgtg cgggtcgggc ctgatcgacc tgttggccga gctgcgccgc cacggccgga
     1861 tgaccgccaa gggcgtgttc gccgacgacc gccgcaagtt cctgatcgag gtcgtgccgg
     1921 agcgcggcat cactttctcc aaggccgacg ccagcgccct cgcccaggcc aaggccgcca
     1981 actactgcgg ccagctgatc gtgctgcgca cgctcggcct cgaccccggc gcggtcgagc
     2041 ggctgttcct cgccggcgga ttcgccacct atgtcgatac cgccaacgcg atcgacatcg
     2101 gcctgctcgc gccggtgccc gaggcggcgg tggtcaaggt cggcaacgcc gcgctgcgcg
     2161 gcgcgcgcga ggtgctgctg tccctgccca agcgccggcg cctggaggcg ctggtccagc
     2221 gcatcgagca tgtcgagctc gagaccaccc ccgatttctt cgacctgttc gtcgacggct
     2281 gccagttccg gccgatgccc gacgtgctca cgccaccgcc gcagcaaagg accgcgggat
     2341 gaagcttgcc gacggccgca ccctgatcgt gatcggcgag aacatccaca ccacgcgcgt
     2401 ggtgctgcgc aagggcaggc gcttcgtcga cgacggcgcg cgccagggcg tgcgctacac
     2461 cgggaacgac ggcgccgagc gcctgctgcc ggtgcccgac tgggcgaagc gcggccagga
     2521 ctaccaggag gggcgggtca agcacgtcat gatcgcggtg cgcgcggcga tggcgggcgc
     2581 gggcgccgag gcggcccagg gcatggacta catcgcgcgc atggtgagcg acctcgaagg
     2641 cgccggcgcc gactatctcg acctaaacgt cgacgagatc tcgatcaagc tcgccgacca
     2701 gaaggcggcg atcgcgtggc tggtgcgcac ggtgcaatcg ctgacagcgc tgccgttgtc
     2761 ggtcgattcg tcgaacctgg aggtgatcga ggccgggctc gccgcctgcg acggcgcgcg
     2821 cgcgcgcccg ctgctcaact cggcctcgct cgagcgcatc gacgccctcg acctggccgc
     2881 cgccgccggc gcccaggtgg tggtcaccgc ggccggcgac tcagggatgc ccgacggcgt
     2941 cgcccagcgc ctcgacaacg cctcgcgcat ggtcgaggcg gcgcgcgcca agggcttcgc
     3001 cttgtccgac atcttcatcg acccgctgct gttcccggtc tcggtggaca ccgtctacgg
     3061 cccgcacggg ctcgacgcga tccgcgccct gcgcgcccgc ttcggccccg acatccacat
     3121 caccggcggc atgagcaacg tctcgttcgg gatcccggcg cgcaaggtca tcaacgaggt
     3181 gttcctgctg ctggcgctgg aggcgggcgc agacagcggc atcatcgatc cggtggcgag
     3241 cccgccgcag gcgctgctcg cgatcgaccg ctcctcgccc tcctacaagc ttgccgagga
     3301 tgtgctgctc gaccgcgaca agcactgccg cagcttcatc cgcgcctggc gcaagggcga
     3361 gctcgccccc ctcgccgccg gcgcctgacc ggcgcgcgcg gggcgatgac cggcctcgcg
     3421 gtcaacctgg tcctgggctt tctcggcgcc ggcaagacca ccctggtcgc gcgattgctc
     3481 gaccatctca aggggcgcga gcgggtggcg ctggtggtca acgagttcgg cgccgtcggc
     3541 atcgacggcg cggtgctcga gggccgcgcc gtcgataccg tcgagctcag ctcgggctgc
     3601 gtctgctgca cgctgaaggc gccgttgcag gcggcgctcg gcgagcttgc cgggcgcggc
     3661 gatatcgacc gggtgatcat cgaggcgagc ggcgtcgccc aggccgccga cctggtcgca
     3721 gcgctggccg aggacgacct gtcgctcggg ccgatcatcg cagtggtcga tgccgcgaga
     3781 ttcgcccagc tgaacgcgat gctcgggccg ttctacgccg gccaggtcgc gcgcgccgac
     3841 gtggtgatcg tcaacaagtg tgacctcgcc ggcgctgcga cgctgtccga ggtggcggag
     3901 gcggtcgccg cgctcaatcc gcgcgcccgc ctcgccttcg ccgagcgctg cgacgtcgat
     3961 cccgccgacg tgctcgccgg tgccgccgcg cctgcggcgg ggctggccga cgaccatttg
     4021 caccaggccg ccgagaccct ggtgctcgcc gcgcccgagg ccgcgcggcg cgccgaggtc
     4081 gagcggttct tcgccgcgct gccgcccggg ctgtggcggg cgaagggctt catgtccatc
     4141 gacggcgcac cgcggctggt ccagtacgcc tgcggggagc tcgagatcgc gccggcgccg
     4201 ccgcgcgcgc agctccacgt ggtgttcgtc ggccgcgccc tcgaccgcgc cgatctcgcg
     4261 cgccggttcg ccgccctcgg gcgctgaggc acgcggctgg acaagcgcgc catcgcccgc
     4321 ttagctgcgc cactcgcgcc caccccccgc aaccgggagc cgccatgttc taccgcccgc
     4381 ccgcgccgca cggcctgcgc cagaacccgt tcaacgccct ggtcatcccg cgcccgatcg
     4441 gctggatctc gtcgctcgat acggagggac gggcgaacct cgcgccctac tcgttcttca
     4501 acgccgtcgc ctacaccccg ccgcaggtga tgttcgccgc caccggcctg cacgagcagg
     4561 gcggcttcaa ggacacggtg gcgaacattc aggcgaccgg cgagttcgta gtcaacctcg
     4621 ccacctggcc gttgcgcgag gcgatgaacg cgtgctcggc gccggcgccg cacgatgtcg
     4681 acgagttcaa gctcgccggc ctcacccccg cgcccgccga gacggtcaaa ccgccgcgcg
     4741 tggcggagag cccggtgcac ctcgagtgcc gccacgtgcg ctcgctcgag ctcctgtccg
     4801 ccaaccccga aagccccaac atggtggtgt tcggcgaggt ggtcggcatc catatcgacg
     4861 ataccgtgat cgcggacggc atggtccgct acgaccggct cgacgtcgtc ggccgcctcg
     4921 gctaccgcga ctacgtgcgg gtcaacgaca tcttcgcgat ggagcgtccc ggctggccgt
     4981 gaggcaggcc cgccggcgcc gattggcgcc tttgcccagc tgcccgacat ggtaagctcg
     5041 ccgccatgac caccgcggcc cgggacgacc tgttcgccgg ctatgaccgc gacgggcatt
     5101 tctgcgagct gttcggccgc gccggcctgc ccgcgccggc gcacacctcg ctgctgcgcg
     5161 agcggctcgc cagctatcgg ctggacaccc tgaagaaacg cgcccgcgac gccgagcgcg
     5221 agctgttcaa cctgggcatc accttcacgg tctattcgga gaaggacgcg gtcgaccgca
     5281 tcctgccgtt cgacccgctg ccgcgagtgc tgtcggcgga cgactggcgg accatcgagt
     5341 cgggcgtcgt ccagcgggtg accgcgatca acctgttcct gcacgacatc tatcaccgcc
     5401 agacgatcct ggccgacggc gtggtgccgc gcgcgctggt gctcggcaac gtcaactacc
     5461 ggcccgagat gcgcgggctc gatctgcctt gcggcaccta tgtccatatc tgcggcatcg
     5521 acatcgtgcg cgacgccgcc ggcggcttcc tggtgctgga ggacaatgcg cgcacgccct
     5581 cgggcgtctc ctacgtggtc gagaaccggc acctgatgct gcgcaccttc cccgacctga
     5641 tgaccgggat cggcatccgc tatgtcagcg actacggccg caggctgcgc gaggcgctgc
     5701 aggagctggc gcccgtcggg gtggccgagc cgcaggtggc gctgctgtcg ccgggggcct
     5761 acaactcggc ctatttcgag cacgtgttcc tggcccgcga gatgggcgtg ccgctggtcg
     5821 agggccgcga cctggtggtc gaggacgagc gcgtgtacat gctgaccacc gagggccgcg
     5881 agccggtcga tgtgatctac cggcgcatcg atgacgactt ccttgacccc gagtgcttcc
     5941 gcgccgacag cctgttgggc gtgcgcggcc tgatgcgcgc ctgtcgcgcc ggcagggtgg
     6001 cgctcgccaa cgcggtcggc accggggtcg ccgacgacaa ggcgatctac gcctacatgc
     6061 cgcgcgtcgt gcgctactac ctcgaccagg acccgatcct gcccaatgtc gagacccaca
     6121 tctgcgccga cgcggacggg cttgcctaca ccctcgacca cgtcaacgag ctggtggtca
     6181 agccggtcgg cgcctcgggc ggctacggca tcaccgtcgg ccccttggcc agccgggtcg
     6241 agctcaaggc gtgccgcaag cgcatcgcgg aacgccccga ggactacatc agccagccga
     6301 tggtcgagct gtcggtgtgc ccgaccctga ccgaccgcgg cgtcgagccc aggcgcgtcg
     6361 acctcaggcc gttcgcggtg accgggcgcg acagctgggt gctgccggga gggcttaccc
     6421 gggtcgcgct caggcgcggc tcgatcgtgg tcaactcgtc gcagggcggc ggctcgaagg
     6481 acacctgggt ccttgcctag ctcattgatc gcgggcttgg gaaacagcct gcttgcgcgg
     6541 ttcgcgagca acgcgttctg gatggcgcgc tacatggagc gggcggagaa cctggcccgc
     6601 ctgctcgacg tcaacgagac cttcgcccgc gacagccagg gggtccagga ctggctgccg
     6661 atcgtgcagc tgcacatcga cgacgaaccc ttcttcaagc tctacgcgca ggccgacgcg
     6721 ccctcggtgc tcgagttcta catcctggaa cggcgcaacc cgaacgcgat cgtctcggcg
     6781 gtggcggtgg cgcgcgagaa cgcgcgcagc ctgcgtcacc tgatcagcac cgagctgtgg
     6841 ctccatctca acatgttcca caaccggctg atggcgatgc ggcccgccga cctcgccctc
     6901 gagaccgtgt ccgagctctg ccggacggtc aaggaggact gccagctcca tgccggcatc
     6961 gccgagggca cgctctatcg cgaccaggtg tggagcttct accggatcgg caagaccatc
     7021 gagcgcctcg accagaccac gcgtctgttg gacatcaagt atcaccgcct cgggccgtcg
     7081 tcggacaagg ccggcgagct gatcgacacc gcccagtgga acgcgctgct gcgctcggcc
     7141 gccggctacc aggcgttccg gcgggtctac ccgaccggca tgcggcgcga gaacgtgatc
     7201 cgcttcttcc tctacgaccg cgccttcccg cgctcggtcg ggctgtgcgt cgatgagctc
     7261 ggcaaggagc tcgacggtct cggccgcgcc aagcgcgcca ccggctggcc ggaggtcgcc
     7321 atgtcgcacg atgcgctgcg cgcactcgtg cgtctcgacg atgccgccgc gccggtggcc
     7381 gaaggcgacc tgcaccggca catcgacgac atccagctcg gcctgatcag gctcacggac
     7441 gccctcggcc gcgcgttcct cggcgcccgg cccgacga
//
LOCUS       DASWHU010000183         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_459265, whole genome shotgun sequence.
ACCESSION   DASWHU010000183 DASWHU010000000
VERSION     DASWHU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459265"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VGB88_06760"
     CDS             complement(<1..1383)
                     /locus_tag="VGB88_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9647181.1"
                     /translation="MHPEIVRDSPGACPICGMALEPVMVPAGSGPSPELADMSRRLRI
                     GAGLTAALLVISMGELVPGLAALVEGAWNRWAQLALATPVVVWAGWPFFVRGWRSLVT
                     RSLNMFTLIALGTGTAYAYSLVAVAAPDVFPAGFRNPAGEVGLYFEAAAVITVLVLLG
                     QVLELRARERTSDALRALLDLAPKTARRLRPDGGEDTVALAEVEVGDRLRVRPGESVP
                     VDGVVLDGHSAVDESMVTGESIPVEKAAGDSLIGGTLNGSGGLVMRADKVGRDTMLAR
                     IVGMVAEAQRSRAPIQRLADTVAGYFVPAVVAVAVLSFTGWALWGPPPALAYGLVAAV
                     SVLIIACPCALGLATPMSIMVGTGRGAQAGVLIRNAEALERFEKVDTLVVDKTGTLTL
                     GKPKLVGVVAVDGQDEDSLLALAAGLERASEHPLAEAIVAGAEDRGLSLAAPTDFRSV
                     TGKGVVGRIGG"
     gene            1670..2284
                     /locus_tag="VGB88_06765"
     CDS             1670..2284
                     /locus_tag="VGB88_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647182.1"
                     /translation="MSAAQPFPAGTIDWSGENPGMYLKETPDGPFVSLVSFFRVVLSP
                     HGRGHGLVLLEAPKAAESRPDALNVCVTDNEPLARYLMANFVCHFGAFRGQPAVAAVE
                     YRRLDAVETEGDSRSRYQERVTGQGIEAVLTWDELGPPFMVDMPADRGATGAHRMFSL
                     FVDCHRATAEINGRALKGRSQPRDFAGRTSTTAFLAFSETWLRV"
     gene            2369..2836
                     /locus_tag="VGB88_06770"
     CDS             2369..2836
                     /locus_tag="VGB88_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEX9647183.1"
                     /translation="MLRLNRLTDYGVVVMVQLARADEVLTAQRLAERTSLPQPTVAKL
                     LALLARGGLAAAQRGAAGGYALARAPEDISVADIIETLEGPVALTACVDGANHLCSLE
                     NVCAMRGHWDTVNAAIRRALEGVTLEAMAHAGEPFLFAAAGDARLDPDRTDRL"
     gene            2847..>4207
                     /gene="sufB"
                     /locus_tag="VGB88_06775"
     CDS             2847..>4207
                     /gene="sufB"
                     /locus_tag="VGB88_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9647184.1"
                     /translation="MTLETNDTAAARQVRETTAQGYKYGFFTDISTETAPKGLNEDTV
                     RFISAKKDEPAWMLDWRLKAYRHWLTLGHEEPKWARVSYPPIDYQDAYYYAAPKAKEG
                     PKSLDEVDPELIEAYSKLGIPLAEQERLAGVAVDAVFDSVSVATTYKKKLEEVGVIFC
                     AISEAIREHPELVRSYLGSVVPYTDNFFATLNSAVFSDGSFVYVPKGVRCPMELSTYF
                     RINAAQTGQFERTLIIADAGAYVSYLEGCTAPMRDENQLHAAVVELVALDDAEIKYST
                     VQNWYPGDEQGRGGIYNFVTKRGDCRGRNSKISWTQVETGSAITWKYPSCILRGDDSV
                     GEFYSVALANHRQQADTGTKMIHMGRNTRSTIISRGISAGHGQNAYRGLVKVLPGADG
                     ARNHTQCDSLLMSEHCGAHTFPYIEVRNPTAVVEHEATTSRISDDQLFYFRSRGIGAE
                     DAVS"
BASE COUNT          655 a         1527 c         1450 g          575 t
ORIGIN      
        1 cccgccgatg cgcccgacca cgcccttgcc ggtcaccgag cggaagtcgg tcggcgcggc
       61 gaggctgagg ccgcggtcct cggcgccggc gacgatcgcc tcggcgagcg ggtgctcgct
      121 cgcccgctcg aggccggcgg cgagcgcgag cagcgagtcc tcgtcctgcc cgtcgaccgc
      181 caccaccccg accagcttcg gcttgccgag ggtgagcgtg ccggtcttgt cgaccaccag
      241 ggtgtcgacc ttctcgaacc gttcgagggc ctcggcgttc ctgatcagca ccccggcctg
      301 ggcaccgcgc ccggtgccga ccatgatcga catcggcgtc gccaggccga gcgcgcacgg
      361 gcaggcgatg atcagcaccg acaccgcggc caccagcccg taggcgagcg ccggcggcgg
      421 gccccacagc gcccagccgg tgaacgacag caccgcgacc gcgactacgg cgggaacgaa
      481 gtagccggcg acggtgtccg ccaggcgctg gatcggggcg cggctgcgct gggcctcggc
      541 gaccatgccg acgatgcgcg ccagcatggt gtcgcgcccg accttgtcgg cgcgcatcac
      601 caggccgccg ctgccgttga gcgtgccgcc gatcagggaa tcgcccgccg ccttctcgac
      661 cgggatcgac tcgccggtga ccatcgactc atcgaccgcg ctgtggccgt cgagcaccac
      721 gccgtccacc ggcacgctct cgcccgggcg cacgcgcaag cggtcgccga cctcgacctc
      781 ggccagcgcc accgtgtcct cgccgccgtc gggcctaagc cggcgcgcgg tcttgggcgc
      841 gaggtcgagg agcgccctga gcgcgtcgct ggtgcgctcg cgcgcgcgca gctccagcac
      901 ctggccgagc aacaccagca ccgtgatcac ggccgccgcc tcgaagtaca gcccgacctc
      961 gccggccgga ttgcggaagc cggcgggaaa gacgtccggc gccgccaccg ccaccaggct
     1021 atatgcgtag gcggtgccgg tgccgagcgc gatcagggtg aacatgttga ggctgcgcgt
     1081 gaccagcgag cgccagccgc ggacgaagaa cggccagccg gcccagacca ccaccggggt
     1141 ggccagcgcg agctgcgccc agcggttcca cgccccctcg accagcgcgg cgaggccggg
     1201 caccagctcg cccatgctga tcaccagcag ggcggcggtg aggcctgcgc cgatgcgcaa
     1261 gcgccggctc atgtcggcga gctcggggct cggtccggac cctgcgggga ccatgaccgg
     1321 ctccagcgcc atgccgcaga tcgggcacgc gcccggggag tcgcgcacga tctccgggtg
     1381 catcgggcag gtaaagcgac ccgcctccgt cgcggcgggc tgatccggcg cgcggtctgc
     1441 gccgtggccg tggtgcgcct cgcacgcctg gcatgcgggc tggtgccgcg cggtcgcgtg
     1501 ctgtccagtc atcctcgtct ccgtgccggg gtcctcccgg aggcatagat aaggattcca
     1561 gtaactggaa ggtcaagccc ggcgagcgct tgcactccct gcgcccacgc tcttggcacc
     1621 cgggcgggga ttggcctaag gtcgcgcgcc accttgggag gagacggaca tgagcgcagc
     1681 gcaaccgttc cccgccggaa cgatcgactg gtcgggcgag aacccgggca tgtacctgaa
     1741 agagaccccg gacgggccgt tcgtgtcgct ggtgtcgttc ttccgcgtcg tgctgtcgcc
     1801 gcacggccgc ggccacggcc tggtcctgct cgaggcgccc aaggccgccg agagccggcc
     1861 cgacgcgctc aatgtctgtg tcaccgacaa cgagcccctc gcgcgctacc tgatggcgaa
     1921 ctttgtctgc catttcgggg cgttccgcgg ccagccggcg gtggcggcgg tcgagtaccg
     1981 tcgcctcgac gcggtcgaga ccgagggcga cagccgcagc cgctaccagg agcgggtgac
     2041 cggccagggc atcgaggcgg tgctcacctg ggacgagctc ggccctccgt tcatggtcga
     2101 catgccggcg gatcggggcg cgaccggcgc gcaccggatg ttcagcctgt tcgtcgactg
     2161 ccaccgcgcg accgccgaga tcaacgggcg ggcgctcaag gggcgctcgc agccgcgcga
     2221 tttcgccggc cggaccagca ccaccgcgtt cctcgccttc tccgagacct ggctcagggt
     2281 ctgagccgcc gcccggaccc cttgacaccg gggtcggggt ccccacgtga aacttgacta
     2341 atatggtcaa gtatgggatt tcccgcgcat gctgcggctc aacagactga ccgactacgg
     2401 cgtggtggtg atggtgcagc tcgcgcgcgc ggacgaggta ttgaccgcgc agcggctcgc
     2461 cgagcgcacc agcctgcccc agccgacggt ggccaagctc ttggcgctgc tcgcccgggg
     2521 cggcctcgcc gcggcccagc gcggcgccgc cggcggctac gcgctcgcgc gcgcgcccga
     2581 ggacatctcg gtcgccgaca tcatcgagac gctcgagggc ccggtcgcgc tcaccgcctg
     2641 cgtcgacggc gccaaccacc tgtgcagcct ggagaacgta tgcgcgatgc gcggccactg
     2701 ggacaccgtg aacgccgcca tccgccgggc gctggagggc gtgaccttgg aggcgatggc
     2761 gcacgcgggc gagccgttcc tgttcgccgc cgccggcgac gcgcgcctcg acccggaccg
     2821 aaccgacagg ctctagagag accgccatga cccttgagac caacgacacc gccgccgccc
     2881 gccaggtccg cgagaccacg gcgcagggct acaagtacgg cttcttcacc gacatctcga
     2941 ccgagaccgc gcccaagggc ctgaacgagg acacggtgcg gttcatctcg gccaagaagg
     3001 acgagccggc gtggatgctc gactggcggc tgaaggcgta ccgccactgg ctcaccctcg
     3061 gccacgagga gccgaaatgg gcccgggtca gctatccgcc gatcgactat caggacgctt
     3121 attactacgc cgcgcccaag gccaaggagg ggccgaagag cctggacgag gtcgatcccg
     3181 agctgatcga ggcctacagc aagctcggca tcccgctcgc cgagcaggag cggctggcgg
     3241 gcgtcgcggt cgacgcggtg ttcgacagcg tgtcggtggc gaccacctac aagaagaagc
     3301 tcgaggaggt cggcgtcatc ttctgcgcca tctccgaggc gatccgtgag cacccggagc
     3361 tggtgcgcag ctatctcggc tcggtggtgc cctataccga caacttcttc gccacgctga
     3421 attcggcggt gttcagcgac ggctcgttcg tctacgtgcc caagggcgtg cgctgcccga
     3481 tggagctgtc gacctacttc cgcatcaacg ccgcccagac cggccagttc gagcgcaccc
     3541 tgatcatcgc cgacgcgggc gcctatgtga gctacctcga gggctgcacg gcgccgatgc
     3601 gcgacgagaa ccagcttcac gccgcggtgg tcgagctggt cgcgctcgac gacgccgaga
     3661 tcaagtactc gacggtgcag aactggtatc cgggcgacga gcagggccgc ggcggcatct
     3721 acaacttcgt caccaagcgc ggcgactgcc gcgggcgcaa ttcgaagatc tcctggaccc
     3781 aggtcgagac cggctcggcg atcacctgga agtatccgag ctgcatcctg cgcggcgacg
     3841 attcggtcgg cgagttctac tcggtcgcgc tcgccaacca ccggcagcaa gccgacaccg
     3901 gcaccaagat gatccacatg gggcgcaaca cgcgctcgac gatcatctcg agagggatct
     3961 cggcgggcca cggccagaac gcctatcgcg ggctggtcaa ggtgctgccg ggcgcggacg
     4021 gcgcgcgcaa ccacacgcag tgcgactcgt tgctgatgag cgagcactgc ggcgcccata
     4081 cgtttccgta catcgaggtc cgcaatccca ccgcggtcgt cgagcacgag gcgacgacct
     4141 ccaggatctc cgacgaccag ctgttctact tccgctcgcg gggcatcggc gccgaggacg
     4201 ccgtctc
//
LOCUS       DASWHU010000184         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1192898, whole genome shotgun sequence.
ACCESSION   DASWHU010000184 DASWHU010000000
VERSION     DASWHU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192898"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VGB88_06780"
     CDS             complement(<1..1013)
                     /locus_tag="VGB88_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX9647185.1"
                     /translation="MKRYSIFALARNALGHHRGWQQAWRSPAPKPHYRVVIIGGGGHG
                     LATAYYLAKEHGIADVAVVEKGWLGGGNTGRNTTIIRSNYLCDESALIYDHALKLWEG
                     LSGELNFNVMFSPRGVLNLAHDQHEARELRRRVHSNRLNGVDGEWLEAAAVKEFCPIL
                     NIGPDCRYPVVGASLQRRGGIARHDAVAWGFARAADALGVDIIQQCEVTGIRRDRGRV
                     TGVETTRGTIGADKVAIVAAGHTSVIAAMAGLRLPLQSQPLQALVSEPLKPVLDTVVM
                     SNAVHAYVSQSDKGELVIGAGTDGYVSYTQRGSFAIIEDTLRAIVELFPIFSRVRLMR
                     HWGG"
     gene            1146..>1939
                     /locus_tag="VGB88_06785"
     CDS             1146..>1939
                     /locus_tag="VGB88_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647186.1"
                     /translation="MSDHDRRAPAGGLTRRRFMEAAGGAGVLGALVLVAPAAARAQET
                     GAPLKIGVMGPFTGPASRTGDGIRKGTLMALDDARAAGEVPVTLGGQTHDIELVWVDS
                     ESSPEKAVKAVTDAISRQGVQLMVNGWHSSVAMAVMDAEASLHVVHIGHQGESQFISE
                     KINSDPDRYRGWFKGWPSPPIFAGLYGEPLKYFRETGLWTPANTKAAMLVEDTDFGRG
                     WGEALLNSLGNAGFDPLPYDVTAIDETEFTPLLIKYKAQGVSVVGMT"
BASE COUNT          303 a          658 c          696 g          282 t
ORIGIN      
        1 ccgccccaat ggcgcatcag ccgcacccgg ctgaagatcg ggaacagctc gacgatggcg
       61 cgcagggtgt cctcgatgat cgcgaacgag ccgcgctggg tgtaggagac gtagccgtcg
      121 gtgccggcgc cgatcaccag ctcgcccttg tcggactggc tgacataggc gtgcaccgcg
      181 ttcgacatca ccaccgtgtc gagcaccggc ttgagcggct ccgacaccag cgcctgcaag
      241 ggctggcttt ggagcggcag gcgcaggccc gccatcgcgg cgatgacgct ggtgtgcccg
      301 gcggcgacga tggcgacctt gtcggcgccg atggtgccgc gcgtcgtttc cacgcctgtc
      361 acccggccgc ggtcgcgacg gatgccggtc acctcgcact gctggatgat gtcgaccccg
      421 agcgcgtccg ccgcgcgcgc gaatccccac gcgaccgcgt cgtggcgggc gatgccgccg
      481 cgccgttgca ggctggcacc gaccaccgga taacggcagt cgggcccgat attgaggatc
      541 gggcagaact ccttgaccgc cgccgcctcc agccactcgc cgtcgacgcc gttgagccgg
      601 ttcgagtgca cccggcgcct gagctcgcgc gcctcgtgct ggtcatgggc gaggttgagc
      661 acgccgcgcg ggctgaacat gacgttgaag ttgagctcgc cgctcaggcc ctcccacagc
      721 ttgagcgcgt ggtcgtagat cagcgcgctt tcgtcgcaca ggtagttgga gcggatgatg
      781 gtggtgttgc ggccggtgtt gccgccgccg agccagccct tctcgaccac cgccacgtcg
      841 gcaatgccgt gctccttggc gagataatag gcggtggcaa ggccgtggcc gccgccgccg
      901 atgatcacca cccggtaatg cggcttgggc gccgggctgc gccaggcctg ctgccagccg
      961 cggtgatggc cgagcgcgtt gcgcgccagc gcgaagatcg aataccgctt caccgtccct
     1021 cccccgcccg accgatgcca gggcaaatct gtcaaacgcg cagaggctcg gcaacagcgc
     1081 agcatttgat ttgacgcggg ccggcccgat ccgaaagcat gggccggctg cgaggggagg
     1141 cgacgatgag cgatcacgac aggcgggcac cagccggcgg cctgacccgg cggcggttca
     1201 tggaggcggc gggaggcgcc ggcgtgctcg gcgcgctggt gctggtcgcg cccgccgcgg
     1261 cgcgcgcgca agagacgggc gcgccgctca agatcggcgt gatgggcccg ttcaccggcc
     1321 cggcgagccg caccggcgac ggtatccgca aaggcaccct gatggcgctc gacgacgcgc
     1381 gcgccgcagg cgaggtgccg gtgacgctgg gcggccaaac gcatgacatc gagctggtct
     1441 gggtcgacag cgagtcgagc ccggagaagg cggtcaaggc ggtgaccgac gcgatcagcc
     1501 gccagggcgt gcagctgatg gtcaacggct ggcactcgtc ggtggcgatg gcggtgatgg
     1561 acgccgaggc gtcgctgcac gtcgtccaca tcggccacca gggcgagtcg cagttcatct
     1621 ccgagaagat caacagcgac ccggacaggt atcgcggctg gttcaagggc tggccgtcgc
     1681 cgccgatctt cgccggcctc tacggcgagc cgctcaagta cttccgcgag accggcctgt
     1741 ggacgccggc caacaccaag gcggcgatgc tggtcgagga taccgatttc ggccgcggct
     1801 ggggcgaggc gctgctcaac agcctgggca acgccggctt cgatcccctg ccctacgacg
     1861 tcaccgcgat cgacgagacc gagttcacgc cgctgttgat caagtacaag gcgcagggcg
     1921 tgtcggtggt cggcatgac
//
LOCUS       DASWHU010000185         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3058541, whole genome shotgun sequence.
ACCESSION   DASWHU010000185 DASWHU010000000
VERSION     DASWHU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058541"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..506)
                     /locus_tag="VGB88_06790"
     CDS             complement(45..506)
                     /locus_tag="VGB88_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647187.1"
                     /translation="MFAAFVVVAGGAFARGHADDGWDAIAAGDFAAAAAFWRPRADHG
                     ERGAMVGLGHLASLEGRDAEAARWYHRAAVRGDEVAQTLLASAYLEGRGVVRDPKLAY
                     AWYHIAAETGHARAAEARDLAGRWLSAAEQAEARALARSWQLEGMPEAPRQ"
     gene            592..954
                     /locus_tag="VGB88_06795"
     CDS             592..954
                     /locus_tag="VGB88_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647188.1"
                     /translation="MLLAVAMAPPPERRPHPLDRPLARVAALGVFAAMLALLAVIHRD
                     DLFGAPTAATVPASPADLPSPAEAGFAKAGQAYAACLAARHGQIDGMAADGVVTAGQA
                     ALFKTRAEALCRDLANKR"
     gene            complement(965..>1664)
                     /locus_tag="VGB88_06800"
     CDS             complement(965..>1664)
                     /locus_tag="VGB88_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9647189.1"
                     /translation="LAEAILSGLGYGSGRVVVLDDRDPAVLEARLWALADAEAIAPAS
                     FLPMGAKRERLWLALGHLHDAAPAPVDRLPLPPGAPFGAVAVDTKGCTLCLACVGACP
                     TGALRDNRDKPQLGFQESACIQCGLCRATCPESVITLVPRIDFTSAARATAVLNEEEP
                     FACIRCGKPFGVAATVERIVERLSGHAMFAGDAAALDRIRMCEDCRVAVQFEAPAPLA
                     GRPRPKPRTADDVD"
BASE COUNT          208 a          664 c          596 g          196 t
ORIGIN      
        1 gtgagcttgc cggtcatggg tgtcgccgcg tcctccgccg cgagttactg gcgcggcgcc
       61 tccggcatgc cctcgagctg ccagctgcgg gcgagcgcgc gcgcctccgc ctgctcggcc
      121 gcgctcaacc agcgcccggc caggtcgcgc gcctcggcgg cgcgggcgtg cccggtctcg
      181 gcggcgatat ggtaccaggc ataggcgagc ttcgggtcgc ggaccacgcc gcggccctcc
      241 agataagcgc tcgccagcag ggtctgcgcg acctcgtcgc cgcgcaccgc cgcccggtgg
      301 taccagcgcg ccgcctccgc gtcgcggccc tcgagcgagg cgaggtggcc gaggccgacc
      361 atcgcgccgc gctcgccgtg gtcggcgcgc gggcgccaga acgccgccgc ggcggcgaag
      421 tcgccggccg cgatcgcgtc ccagccgtcg tcggcgtggc cgcgggcgaa cgccccgccg
      481 gcgaccacca caaaggccgc aaacatcagg gtcaaggcag cccgcatcat caccggctca
      541 gactatccaa gcggctcaag gctggccagc gccctcgctc cgatggccgt tatgctgtta
      601 gctgtcgcca tggcaccgcc gccggagcgc cgcccgcacc cgctcgaccg gccgctcgcg
      661 cgcgtcgcgg cactcggcgt gttcgccgcc atgctggcgc tgctcgcagt gatccaccgc
      721 gacgacctgt tcggcgcgcc gaccgccgcc accgtcccgg cctcgcccgc cgaccttcct
      781 tcgcccgccg aagcgggctt cgcgaaggcg gggcaggcct acgccgcctg cctcgccgcc
      841 cgccacgggc agatcgacgg catggccgcc gacggggtcg tcaccgccgg ccaggccgca
      901 ctgttcaaga cccgcgccga ggcgctgtgc cgcgacctgg ccaataagcg ctagactgtc
      961 cgcctcagtc cacgtcgtcg gcggtgcgcg gcttcggccg cggccggccg gcgagcggcg
     1021 ccggcgcctc gaactggacc gccacccggc agtcctcgca catccggatg cggtcgagcg
     1081 ccgccgcatc gccggcgaac atcgcgtgcc cggacagccg ctcgacgatc cgctcgacgg
     1141 tcgcggcgac gccgaacggc ttgccgcagc ggatgcacgc gaacggctcc tcctcgttga
     1201 gcaccgcggt cgcgcgcgcg gccgaggtga agtcgatgcg cggcaccagc gtgatcaccg
     1261 actcggggca ggtcgcgcgg cacagcccgc attggatgca cgcgctctcc tggaagccga
     1321 gctgcggctt gtcccggttg tcgcgcagcg ccccggtcgg gcacgcgccg acgcaggcga
     1381 ggcagagcgt gcagcccttt gtgtcgaccg cgaccgcgcc gaacggcgcg ccgggcggca
     1441 gcggcagccg gtcgaccggc gccggcgccg cgtcgtgcag gtggccgagg gcgagccaca
     1501 gccgctcgcg cttggcgccc atcggcagga acgaggccgg cgcgatcgcc tcggcgtcgg
     1561 cgagcgccca cagccgcgcc tcgagcacgg ccgggtcgcg gtcgtccagc accacgactc
     1621 gaccggagcc gtaaccgagc ccactgagga ttgcctcggc gagg
//
LOCUS       DASWHU010000186         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1954067, whole genome shotgun sequence.
ACCESSION   DASWHU010000186 DASWHU010000000
VERSION     DASWHU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954067"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1807)
                     /gene="lepA"
                     /locus_tag="VGB88_06805"
     CDS             complement(8..1807)
                     /gene="lepA"
                     /locus_tag="VGB88_06805"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9647190.1"
                     /translation="MTDLSHIRNFAIIAHIDHGKSTLADRLIERCGGLSQREMADQVL
                     DTLAIERERGITIKAQTVRLAYRARDGRAYTLNLIDTPGHVDFAYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYKAIDQDHEIVVVLNKIDLPAAEPEQVKRQIEEVIGLDASN
                     AVLISAKTGQNIEAVLEALVARLPAPKGEVEAPLKALLVDSWYDSYLGVIVLVRVYDG
                     TLRKGQRVRFMATGIRHQVERVGVFTPKGIEVETLGPGEVGFLTAAIKTVADTQVGDT
                     VTDDRHPTKAALPGFKPSVPVVFCGLYPSDNADFERLRDSLAKLRLNDASFVYEPETS
                     AALGLGFRCGFLGLLHLEIVQERLEREFDLDLVTTAPSVIYRVHLTNGETLELHNPAD
                     LPDAVRIARIEEPWIRATILVPDAYLGNVLGLCTDRRGQQLDLTYVGGRAMAIYRLPL
                     NEVVFDFYDRLKSVSRGYASFDYELDGYRDGDLVKVSILVNGEPVDSLAFMSHRARAE
                     ARGRALCERLKELIPRQLFKIAIQAAIGGRIIARETVSALRKDVTAKCYGGDVTRKRK
                     LLEKQKRGKKRMRQVGNVEIPQEAFLAALKLET"
     gene            complement(1838..>4253)
                     /gene="pheT"
                     /locus_tag="VGB88_06810"
     CDS             complement(1838..>4253)
                     /gene="pheT"
                     /locus_tag="VGB88_06810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9647191.1"
                     /translation="FTLSWLKDHLDTEASLAQIVDKLTMIGIEVEEVDDPSETFAPFT
                     VASVISAEQHPNADRLRVCMVDTGKGVVQVVCGAPNARAGMKGVFAPLGARLPGTGLV
                     LEKRAIRGVTGYGMLCSEREMGLSDEHEGIIELPEDAPVGTPFAALLGLDDPVLDVAV
                     TPDRGDCLGVLGVARDLAAAGLGRLATPPVAPVEATYASPIGVRLDFAPDAASACPLF
                     IGRLVRGVRNGASPDWLQRRLRAVGLRPISALVDVTNFVTFDRARPLHVFDADALAGS
                     IHVRLARAGERLAALDERSYELDAEMCVVADDDGPVALGGVIGGQATACTAETANVFV
                     EAAWFDPVRTAATGRKLGIESDARYRFERGVDRAGTAPGIELATRMIVELCGGEPSEL
                     VVAGGEPPWGRTLDFHPARVHSLGGLDLPKDDSIRILDALGFAPDDRGEEVSVRVPSW
                     RNDIDGEADVVEEVLRVEGFDSIPPVSLPRESAVARPALDAAQRRVRDARRVLAARGL
                     DECVTWSFLSAPLAALFGGGAQQLKLANPISSELTDMRPSTLPNLIAAAVRNAARGVT
                     GGGLFEVGPVYADDTPEGQATMAGGIRTGRTGPRHWLADARPVDAFDAKADALAVLAA
                     LGLEADKMTVTVDAPGWYHPGRSGVIRLGPKTALATFGEVHPRVLKKMDAPAPMVAFE
                     VHLAGVPLPKPRPTRARPPFAPSDLPAVSRDFAFVVAQSVAAAKVLGAVRALSGRPPH
                     RLAFTAVELFDVYTGEGVPDGHKSLAVAVTLQPAQRTLTEDDIQAVSDAIVAQVEKAT
                     GGRLRT"
BASE COUNT          599 a         1481 c         1555 g          618 t
ORIGIN      
        1 atggacgcta tgtctccagc ttgagcgcgg cgaggaacgc ttcctgcggg atctcgacgt
       61 tgccgacctg gcgcatccgc ttcttgccgc gcttctgctt ctccagcagc ttgcgcttgc
      121 gggtgacgtc gccgccgtag cacttggcgg tgacgtcctt gcgtagcgcg ctcacggttt
      181 cgcgggcaat gattcgcccg ccgatcgcgg cttggattgc gatcttgaac agctggcgcg
      241 ggatcagctc cttgagccgc tcgcacagcg cgcgcccgcg cgcctcggcg cgcgcgcgat
      301 ggctcatgaa ggcgaggctg tcgacgggct cgccgttgac caggatcgac accttgacca
      361 ggtcgccgtc gcggtagccg tcgagctcgt agtcgaagct ggcatagccg cggctcaccg
      421 acttgagccg gtcgtagaag tcgaacacca cctcgttcag cggcaggcgg tagatcgcca
      481 tcgcccggcc gccgacatag gtgaggtcga gctgctggcc gcggcggtcg gtgcacagcc
      541 cgagcacatt gccgagatag gcgtcgggca ccaggatggt ggcgcggatc cacggctcct
      601 cgatgcgcgc gatgcgcacc gcgtcgggga ggtccgcggg gttgtgcagc tccagcgtct
      661 cgccgttggt caggtgcaca cggtagatca cgctcggcgc ggtggtcacc aggtcgaggt
      721 cgaactcgcg ctcgagccgc tcctggacga tctcgagatg gaggaggccg aggaatccgc
      781 agcggaagcc gaggcccagc gcggccgagg tctcgggctc gtagacgaag ctcgcgtcat
      841 tgagcctgag cttcgccagg ctgtcgcgca gccgctcgaa gtcggcgttg tcggacgggt
      901 acaggccgca gaacaccacc ggcacgctcg gcttgaagcc gggcagcgcc gccttcgtcg
      961 ggtggcggtc gtcggtgacg gtatcgccga cctgggtgtc ggcgacggtc ttgatcgcgg
     1021 cggtaaggaa gccgacctcg cccgggccga gcgtctcgac ctcgatcccc ttgggcgtga
     1081 atacgccgac ccgctcgacc tggtggcgga tgccggtggc catgaagcgc acccgctggc
     1141 ccttgcgcag ggtgccgtcg tagacccgga ccagcacgat gacgccgaga taggagtcgt
     1201 accagctatc gaccaggagc gccttcaggg gcgcctcgac ctcgcccttg ggcgcgggca
     1261 ggcgcgcaac cagcgcctcg agcacggcct cgatgttctg gccggtcttg gccgagatca
     1321 gcaccgcgtt ggaggcgtcg aggccgatca cttcctcgat ctggcgcttg acctgctcgg
     1381 gctcggccgc gggcaggtcg atcttgttga gcacgaccac gatctcgtgg tcctggtcga
     1441 tcgccttgta gacgttggcg agggtctgcg cctccactcc ctggctcgcg tcgaccacca
     1501 acagcgagcc ctcgcaggcg gcgaggctgc ggctgacctc gtaggcgaag tcgacatggc
     1561 ccggggtgtc gatcaggttg agggtatagg cgcgcccgtc gcgcgcccgg taagcgaggc
     1621 gcacggtctg ggccttgatg gtgatgccgc gctcgcgctc gatcgccagc gtgtcgagta
     1681 cctggtccgc catctcgcgt tggctgagac cgccgcagcg ctcgatcagc cggtcggcca
     1741 aggtcgactt gccgtggtcg atatgggcga tgatcgcgaa gttgcggatg tgcgacaggt
     1801 cggtcatggc cgggcgcgcg ggcgcaggcg acctccgtca ggtgcgcaac cgcccgccgg
     1861 tcgccttctc gacctgggcg acgatcgcat ccgagaccgc ctggatgtcg tcctcggtca
     1921 gcgtgcgctg ggcgggctgc agggtgaccg cgacggcgag cgacttgtgg ccgtcgggca
     1981 cgccctcgcc ggtatagacg tcgaacagct cgacggcagt gaacgcgagc cggtgcggcg
     2041 gcctgcccga cagcgcgcgc accgcgccga gcaccttggc ggcggcgacc gactgcgcga
     2101 ccacgaaggc gaagtcgcgg ctcaccgccg gcaggtctga gggggcgaag ggcgggcggg
     2161 cgcgggtcgg gcgcggcttg ggcagcggca cgccggcgag gtggacctcg aacgcgacca
     2221 ttggcgccgg cgcgtccatc ttcttgagca cgcgcgggtg gacctcgccg aaggtggcga
     2281 gcgcggtctt gggcccgagc cggatcacgc ccgaccgccc ggggtggtac cagccgggcg
     2341 catcgacggt gaccgtcatc ttgtcggcct cgagcccgag cgcagcgagc accgccagcg
     2401 catcggcctt ggcgtcgaag gcatcgaccg gccgcgcgtc ggccagccag tggcgcgggc
     2461 cggtgcgccc cgtgcggatg ccgccggcca tggtcgcctg cccttcgggc gtgtcgtccg
     2521 cgtagaccgg gccgacctcg aacagtccgc cgcccgtgac cccgcgggcg gcgttgcgca
     2581 ccgccgccgc gatcaggttg gggagcgtcg aggggcgcat gtcggtgagc tcgctcgaga
     2641 tcgggttggc gagcttgagc tgctgcgcgc cgccgccgaa cagcgcggcg agcggcgccg
     2701 acaggaacga ccaggtgacg cactcgtcga gcccgcgcgc agccagcacc cggcgcgcgt
     2761 cgcgcacgcg ccgctgggcg gcgtcgagcg ccgggcgcgc aaccgcggac tcgcgcggca
     2821 gggacactgg cgggatcgaa tcgaaccctt cgactctcag cacctcctcg acgacatcgg
     2881 cctcgccgtc gatatcgttg cgccacgacg gcacccgcac cgagacctcc tcgccgcggt
     2941 cgtcgggggc gaagccgagc gcgtcgagaa tccggatgct gtcgtccttg ggcaggtcga
     3001 gcccgccgag cgaatgcacg cgcgccggat ggaagtcgag cgtccggccc cacggcggct
     3061 cgccgccggc gaccaccaat tcgctcggct cgccgccgca cagctcgacg atcatgcgcg
     3121 tcgcgagctc gatgcccggc gcggtgccgg cgcggtcgac gccgcgctcg aagcggtagc
     3181 gcgcgtccga ctcgatgccg agcttgcgcc cggtcgccgc ggtacgcacc gggtcgaacc
     3241 acgccgcctc gacgaatacg ttggcggtct cggcggtgca cgcggtggcc tggccgccga
     3301 tcaccccgcc gagcgcgacc gggccgtcgt cgtcggcgac cacgcacatc tccgcatcga
     3361 gctcgtagct gcgctcgtcg agcgcggcca gccgctcgcc ggcgcgcgcg aggcggacgt
     3421 ggatcgatcc cgccagcgca tcggcgtcga acacgtgcag cggccgcgcc cggtcgaagg
     3481 tgacgaagtt ggtgacgtcg accagcgccg agatcggcct caggcccacc gcgcgcagcc
     3541 gtcgctgcag ccagtccggg ctcgcaccgt tgcgcacgcc gcgcaccagg cggccgatga
     3601 acagcggaca ggccgaggcg gcatcgggcg cgaaatcgag gcgcaccccg atcgggctcg
     3661 cgtaggttgc ctccaccggc gcgaccggcg gggtggcgag ccggccgagg ccggcggcgg
     3721 cgaggtcgcg ggcgacgccg agcacgccca ggcagtcgcc gcggtccggc gtcacggcga
     3781 cgtcgagcac cgggtcgtcg aggccgagca gggcggcgaa cggcgtgccc actggggcgt
     3841 cctcgggcag ctcgatgatg ccctcgtgct cgtcgctcaa gcccatctcg cgctcggagc
     3901 acagcatgcc gtagccggtc accccgcgga tcgcgcgctt ctcgagcacg agcccggtcc
     3961 ccggcaggcg cgcgccgagc ggcgcgaaca cgcccttcat gccggcgcgc gcattgggcg
     4021 ctccgcagac gacctgcacg acgcctttac cggtatcgac catgcagacc ctgagccggt
     4081 cggcgttggg gtgctgctcg gcgctgatga ccgaggcgac cgtgaagggc gcgaatgtct
     4141 ccgaagggtc atcgacctcc tcgacctcga tgccgatcat ggtgagcttg tcgacgatct
     4201 gggccaagct cgcctcggtg tcgagatggt ccttcagcca ggacagggtg aac
//
LOCUS       DASWHU010000187         8542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_88410, whole genome shotgun sequence.
ACCESSION   DASWHU010000187 DASWHU010000000
VERSION     DASWHU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88410"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VGB88_06815"
     CDS             <1..742
                     /locus_tag="VGB88_06815"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647192.1"
                     /translation="HRGASAATPAKAAAGRDIVFVCVGNDDDVRSVVYGDDGAFAGMR
                     KGAILVDHTTDSANLARELSAAAAGRGLGFLDAPVSGGQAGAENGALTVMVGGEQATF
                     DRAAPVIEAYARAVTLMGPAGSGQLTKMVNQICIAGLVQGLAEGMNFAARAGLDTDKV
                     VDVISKGAAGSWQMENRARTMAADKFDFGFAVNWMRKDLGITLEEAQGNGARLPVTAL
                     IDQFYAQVQARGGGRWDTSSLIHLLAHD"
     gene            complement(750..1727)
                     /locus_tag="VGB88_06820"
     CDS             complement(750..1727)
                     /locus_tag="VGB88_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007426725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX9647193.1"
                     /translation="MTPLLIAGGLLALLAGAEVMLRGAVALATRLRMSQALIGLTLVG
                     FGTSTPELLTSLEAARLGSPGIAVGNVVGSNIANVLLILGVGAVLAPIAAPRAAVIRD
                     GSAMVAASLLFVAVCLYGALTRPVGAGFVALLAGFIAYSIWREHRARDPAGELHAAET
                     ALMEPAPSRIPLALGMFAVGLAGLLAGAHWLVEGGVALAVALGVSETVIGLTIIAVGT
                     SLPELAATGVAALRGRTDIALGNILGSNLFNILGILGLTAALTELAIPADIAGFDAWV
                     MLGAALLLMAVAWTGSVVSRREGVALLVLYAGYAALISVPGWRALVIGD"
     gene            complement(1724..2533)
                     /gene="mazG"
                     /locus_tag="VGB88_06825"
     CDS             complement(1724..2533)
                     /gene="mazG"
                     /locus_tag="VGB88_06825"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX9647194.1"
                     /translation="MSAIDRLLDIMARLRDPESGCPWDLAQSFATIVPHTIEEAYEVA
                     EAIARDDMPGLADELGDLLFQVVFYAQMAREAGAFTFDDVARGIANKMLRRHPHVFAD
                     ATISTAEQQTDSWEALKAEERAARAEAGRPPSALDGVARALPALARAAKLQRRAARVG
                     FDWPEVRPVLAKVREEIDEVEAEFDRVADAEAARARLADEVGDVLFACANLARHAGVD
                     PESALRGANDKFERRFRRIEALLAAAGKRAEDCTLAELDRLWTRAKAEEDR"
     gene            complement(2729..3025)
                     /locus_tag="VGB88_06830"
     CDS             complement(2729..3025)
                     /locus_tag="VGB88_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647195.1"
                     /translation="MAADGALVMAMLSGALVDPVLWVIGAVIGWDHRRAVQTTAALLV
                     AGGLVWGAVRVGVYIAFGEALGASRAALLVLICVALMVALGLAVREGRWLLANR"
     gene            complement(3045..3833)
                     /locus_tag="VGB88_06835"
     CDS             complement(3045..3833)
                     /locus_tag="VGB88_06835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9647196.1"
                     /translation="MLVDSHCHLDFPDFQDDLEGVIGRARAAGVVTIQTICTRLSKFD
                     DVRALTERFDDLYCSVGVHPHNAAEEAPDGVDDLVGRTRHAKVIGIGETGLDYYYQHS
                     PRAAQERSFRIHIEAARVTGLPVIVHTRDADADTIAVLADEMAKGAFPGLIHCFSAGR
                     EVAEKAIELGMMVSLSGIVTFAKAEALRAIVRDLPLDHLLVETDAPYLAPVPRRGKRN
                     EPAFVAETARYLAGLLGVDFDALAAQTSRNFFKLFAKARPALAA"
     gene            complement(3833..5392)
                     /gene="metG"
                     /locus_tag="VGB88_06840"
     CDS             complement(3833..5392)
                     /gene="metG"
                     /locus_tag="VGB88_06840"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9647197.1"
                     /translation="MPGATAFYVTTPIYYVNDVPHLGHAYTTIACDVLARFARLDGRR
                     VMFLTGTDEHGQKVEKSAASAGEAPQAFTDRVSGRFRALTAALGISNDDFIRTTEPRH
                     ARASQAIWRALVDAGDIYLDTYAGWYSVRDEAYFAESELVDAPGGGKLAPSGAPVEWV
                     EEPSYFFRLSRWQQPLLDHYRRDPDAILPAGRRNEVLSFIRGGLQDLSVSRTSFKWGV
                     PVPGDDAHIIYVWLDALTNYLTAVGYPDTDSEPFRTFWPADVHVVGKDILRFHAVYWP
                     AFLMAAGVAPPRRVFAHGWWTVEGQKMSKSLGNVVDPFGLVETYGLDQVRYFLLREVP
                     FGNDGDFSHAALVHRTNGDLANDYGNLAQRALAMVHRHCGAAVPEAGPFTDDDRALLD
                     AASRLVEVLRPLMERQAPHEALDAIWAVVRAANGYVDRQAPWVLRKDDPARMGTVLYA
                     VAECLRHLAILTQPFMPDASARLLDQLAVPPGARDFSHLGPRHALAQGAALPAPEGVF
                     PRRGETAEAAS"
     gene            complement(5427..6515)
                     /locus_tag="VGB88_06845"
     CDS             complement(5427..6515)
                     /locus_tag="VGB88_06845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX9647198.1"
                     /translation="MADTDLDLPPAPRANPDLVGHAAAEATLRRGFESGRLAHAWLIA
                     GPRGVGKATLAYRFARYVLAGGAVQADLVGGGEAGLALDADHPVFRRVASGGHRDLLT
                     IERGVDSKGRERSEVVVADARRLAEFLALTPAEGGWRVAVIDAADEMNRNAVNAVLKL
                     VEEPPRRALVLLVCHVPGRVPRTLRSRCRMLTLAPLAADAVAELLRRWRPALAPDAAG
                     QLARLAEGCPGRALALADSDGLALYRDLVGLIASAPGIDARRLHGFGDRLARRGGEDA
                     FATAMALLAGWLARLVAAGARGAAPEPLVAGEGEAARRLLAARGLDQWVELWEKVVDL
                     AARTHWVNLDRKQVVLSIFAALERAVRA"
     gene            complement(6502..7152)
                     /gene="tmk"
                     /locus_tag="VGB88_06850"
     CDS             complement(6502..7152)
                     /gene="tmk"
                     /locus_tag="VGB88_06850"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9647199.1"
                     /translation="MTRGRLVTVEGGEGAGKSTQCAHLARSLARAGIEAITTREPGGA
                     PGAEAIRALLVDGALDRWDPTAEALLHFAARREHLIRTVWPALAAGKWVVCDRFADST
                     MAYQGCAMGVGRETVEALYRVAVGDFAPDLTLILDVPVAVGLARAAARAGPPQRYERM
                     DNAFHERLRKAFLDIARRAPDRCVMIDASLPEDAVARQAFAVVRDRLGIEVPDGGH"
     gene            complement(7149..8318)
                     /locus_tag="VGB88_06855"
     CDS             complement(7149..8318)
                     /locus_tag="VGB88_06855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9647200.1"
                     /translation="MIHTVPKTRCRASRGQAWLALLPLLLLAWPAQAFDTAAREAILI
                     DFDTGAVLLDKNADTPAPPASMSKLMTIYMVFERLKAGTLTLDDPLPVSEKAWRTGGS
                     KMFVKVDTNVTVGDLLQGIIVQSGNDACVVVAEALAGSEQAFAQKMTERAREIGLTNS
                     AFTNSYGWPDPGHYMSPRDLAHLTERLIRDFPDYFHFFAETEFTYNGIKQGNRNPLLY
                     KEGLGADGMKTGHTEDAGYSLTATAEREGRRLILVITGLGSVSDRSAEAERILNWGFR
                     DTRNYALFKAGETVAEAPVWLGEEARVPLVIVDPLVVTLRRDSRDDMRVAVVADAPVP
                     APIVAGTRLATLTVTAPEAQPVQVPLVAGADVGQLGVVARLLSALRYLVFGPPTS"
BASE COUNT         1278 a         3043 c         3090 g         1131 t
ORIGIN      
        1 gcaccgcggc gccagcgccg cgactccggc caaggcggcg gcgggacgcg acatcgtgtt
       61 cgtctgcgtc ggcaacgatg acgacgtgcg cagcgtcgtc tacggcgacg acggcgcgtt
      121 cgccggcatg aggaagggcg cgatcctggt cgaccacacc accgactcgg ccaacctcgc
      181 gcgcgagctt tcggcggccg cggccgggcg cggcctcggc ttcctcgatg cgccggtctc
      241 gggcggccag gccggcgccg agaacggcgc gctgacggtg atggtcggcg gcgagcaggc
      301 gacgttcgac cgcgcggcgc cggtgatcga ggcttacgcc cgcgcggtca ccctgatggg
      361 cccggcgggc agcggccagc tgaccaagat ggtcaaccag atttgcatcg ccggcctggt
      421 ccaggggctc gccgagggca tgaacttcgc ggccagggcg gggctcgaca ccgacaaggt
      481 ggtcgacgtc atctcgaagg gcgccgcggg ctcgtggcag atggagaacc gggcccggac
      541 catggccgcc gataagttcg atttcgggtt cgcagttaac tggatgcgca aggaccttgg
      601 aattaccctc gaggaagctc aaggcaacgg cgcgcgcttg ccggtcaccg cgctgatcga
      661 ccagttctac gcccaggtgc aggcgcgcgg cggcggccgc tgggacacct cgagcctgat
      721 ccacctgctc gcccacgatt gagcggggat cagtcgccga tcaccagcgc gcgccagccc
      781 ggcaccgaga tcagcgcggc gtagccggcg tagaggacga gcaaagccac gccctcgcgc
      841 cggctgacga cgcttccggt ccaggccacc gccatcagca acagcgccgc gccgagcatc
      901 acccaggcgt cgaagccggc gatatcggcc gggatggcga gctcggtgag cgccgcggtc
      961 aggccgagga tgcccaggat gttgaacagg ttgctgccga ggatgttgcc gagcgcgatg
     1021 tcggtgcgcc cgcgcagggc ggcaacgccg gtggcggcga gctccggcag cgacgtgccg
     1081 accgctatga tggtgagccc gatcacggtc tcgctgacgc cgagcgcgac ggcgagcgcg
     1141 acgccgccct cgaccagcca gtgggcaccg gccaaaagtc cggcaaggcc gaccgcgaac
     1201 atgccgagcg cgagcgggat gcgcgacggc gccggctcca tcagcgcggt ctcggccgcg
     1261 tgcagctcgc ccgcgggatc gcgggcgcga tgctcgcgcc atatgctgta ggcgatgaac
     1321 ccggcgagca aggcgacgaa cccggcgccg accggccggg tgagcgcgcc gtacaggcac
     1381 acggcgacga acagcaggct cgccgcgacc atcgccgagc cgtcgcggat caccgccgcg
     1441 cgcggcgcgg cgatcggcgc cagcactgcg ccgacgccga gaatcaggag cacgttggcg
     1501 atgttgctac cgaccacgtt gccgaccgcg atgccgggcg agccgagccg cgcggcctcg
     1561 aggctggtga gcagctccgg tgtcgaggtg ccgaagccga ccagggtgag gccgatcagc
     1621 gcctgcgaca tcctgagccg ggtggcgagc gcgaccgcgc cgcgcagcat cacctcggcg
     1681 ccggcgagca gcgcgagcag gccgccggcg atcaacagcg gggtcatcgg tcctcttcgg
     1741 cctttgcccg ggtccacagc cggtcgagct cggcgagcgt gcagtcctcg gcgcgcttgc
     1801 cggcggcggc gagcagcgcc tcgatgcgcc ggaagcggcg ctcgaacttg tcgttggcgc
     1861 ccctaagcgc gctctcggga tcgacgccgg cgtggcgggc gaggttggcg caggcgaaca
     1921 gcacgtcacc gacctcgtcg gccaggcgtg cccgcgcggc ctcggcgtcg gcgacgcggt
     1981 cgaactctgc ctcgacctcg tcgatctcct cgcgcacctt ggccagcacc gggcgcacct
     2041 cgggccagtc gaagccgacc cgggcggcgc gccgttgcag cttggcggca cgggcgaggg
     2101 cgggcagcgc gcgggcaacg ccgtcgagcg cgctgggcgg tcgcccggcc tcggcgcgcg
     2161 cggcgcgctc ctcggccttc agcgcctccc agctatcggt ctgctgctcg gcggtcgaga
     2221 tggtcgcgtc ggcgaacacg tgcgggtggc gcctgagcat cttgttggcg atgccgcggg
     2281 cgacgtcgtc gaaggtgaag gcgccggcct cgcgcgccat ctgggcgtag aacaccacct
     2341 ggaacagcag gtcgccgagc tcgtcggcaa gccccggcat gtcgtcccgg gcgatcgcct
     2401 cggcgacctc gtaggcttcc tcgatggtgt gcgggacgat ggtcgcgaaa ctctgcgcaa
     2461 ggtcccacgg gcagccggac tccgggtcgc gcaagcgcgc catgatgtcg agcagccgat
     2521 cgatcgcgct catcaacagc ctctcgtatg gctggtgcct ggcgaggcgg gacgctgctg
     2581 ggccacgctt gagttgagga tcggcataag gaaaagtcgc ataaggtata ttatgggaaa
     2641 taatatcgtc atcgaagccg ttgatccggc tcggcttccc gcgccacgcg ccggccgccg
     2701 gccccaaatc ggcgacgcgt cggtatcgtc atcggttggc gagcagccag cgcccttcgc
     2761 gcaccgcaag cccgagcgcg accatcagcg cgacacagat cagcaccagc agcgccgcgc
     2821 gcgatgcccc gagggcttcg ccgaacgcga tgtagacgcc gacgcgcacc gcgccccaca
     2881 ccaacccgcc cgcgaccaat agcgcggcgg tggtctgcac cgcgcgccgg tggtcccagc
     2941 cgatgaccgc gccgatcacc cacagcaccg gatcgaccag tgccccggac agcattgcca
     3001 tgaccaaggc cccgtcggca gccatcggcc ctattccgag gcggtcatgc ggcaagcgcg
     3061 ggccgcgcct tggcgaacag cttgaagaag ttgcggctgg tctgggcggc aagcgcgtcg
     3121 aaatcgaccc ccagcagccc ggcgagatag cgcgcggtct cggcgacgaa ggcgggctcg
     3181 ttgcgcttgc cccgccgcgg caccggggca aggtacggcg cgtcggtctc gaccagcagg
     3241 tggtcgagcg gcaggtcgcg cacgatcgcg cgcaaggcct cggccttggc gaaggtgacg
     3301 atccccgata gcgacaccat catgccgagt tcgatcgctt tttccgccac ttcgcgcccg
     3361 gcgctgaagc aatggatcag gccgggaaac gcgcccttgg ccatttcgtc cgcgagcacg
     3421 gcgatggtgt ccgcatcggc gtcgcgggta tggacgatga ccggcaggcc ggtgacgcgc
     3481 gccgcctcga tatggatgcg gaagctccgc tcctgcgccg cgcgcgggct gtgctggtag
     3541 taataatcta ggccggtctc gccgatgccg atcaccttgg cgtggcgggt gcgcccgacc
     3601 aggtcgtcga cgccgtcagg cgcctcctcg gccgcattgt gcggatggac gccgaccgag
     3661 cagtagaggt cgtcgaaccg ctcggtgagc gcgcgcacgt cgtcgaactt gctcaagcgc
     3721 gtgcagatgg tctgtatcgt caccaccccg gcggcgcgcg cgcgcccgat gactccctcc
     3781 aggtcgtcct ggaagtcggg gaagtcgagg tggcaatggc tgtcgaccag catcacgacg
     3841 cggcctcggc ggtctcgccg cggcgcggga acaccccttc cggcgccggc agcgccgccc
     3901 cttgggcgag cgcatgtcgc ggcccgagat ggctgaagtc gcgtgctccc ggcggcaccg
     3961 cgagctggtc gagcagccgc gcggaggcat cgggcatgaa cggctgggtc aggatggcga
     4021 ggtggcgcag gcactcggcg accgcgtaca gcaccgtgcc catgcgcgcc gggtcgtcct
     4081 tgcgcagcac ccacggcgcc tggcggtcga catagccgtt ggccgcccgc accaccgccc
     4141 agatcgcgtc cagcgcctcg tgcggcgcct ggcgctccat cagcggccgc aggacctcga
     4201 ccagccgcga ggcggcatcg agcaacgcgc ggtcgtcgtc ggtgaacggc ccggcctcgg
     4261 gcaccgccgc gccgcaatgg cggtggacca tggccagcgc ccgctgcgcc aggttgccgt
     4321 agtcgttggc caggtcgccg ttggtgcggt ggaccagcgc cgcgtgcgag aagtcgccgt
     4381 cgttgccgaa cggcacctcg cgcagcagga agtagcggac ctggtcgagg ccataggtct
     4441 cgaccagacc gaacggatcg accacgttgc cgaggctctt cgacatcttc tggccctcga
     4501 ccgtccacca gccgtgggcg aacacgcgcc tgggcggcgc gacgcccgcg gccatcagga
     4561 atgccggcca gtagaccgcg tggaagcgaa ggatgtcctt gccgacgaca tggacgtcgg
     4621 ccggccagaa ggtgcggaac ggctcgctgt cggtgtcggg atagccgacc gcggtcaggt
     4681 agttggtcag cgcatcgagc cagacgtaga tgatgtgcgc gtcgtcgccc ggcaccggca
     4741 cgccccactt gaagctggtg cgcgacaccg acaggtcctg gagcccgccg cggatgaagc
     4801 tcagcacctc gttgcgccgg ccggcgggaa ggatcgcgtc gggatcgcgc cggtagtgat
     4861 cgagcagcgg ctgctgccag cgcgacaggc ggaagaagta gctcggctcc tcgacccatt
     4921 cgaccggcgc tcccgacggc gccagcttgc cgccgcccgg tgcgtcgacc agctcgctct
     4981 cggcgaaata ggcctcgtcg cgcaccgagt accagccggc ataggtatcg aggtagatgt
     5041 cgccggcgtc caccagcgcc cgccagatcg cctggctggc gcgggcgtgg cgcggctcgg
     5101 tggtgcggat gaagtcgtcg ttggagatgc cgagcgcggc ggtcagcgcg cggaagcgcc
     5161 cggacacccg gtcggtgaac gcctgcggcg cctcgcccgc cgatgccgcc gacttctcga
     5221 ccttctggcc gtgctcgtcg gtgccggtga gaaacatcac ccgccgtccg tccaggcgcg
     5281 cgaaccgcgc cagcacgtcg caggcgatgg tggtgtaggc gtggccaaga tggggcacgt
     5341 cgttaacgta gtagatcggc gtggtgacgt agaaggccgt ggcgcccggc atcccgataa
     5401 gctcctgggc tcagatcaga gaagagtcac gcgcgcacgg cgcgctcgag cgcggcgaag
     5461 atgctcagca ccacctgctt gcggtcgaga ttgacccagt gggtccgcgc ggcgagatcg
     5521 acgacctttt cccacagctc cacccattga tcaaggccgc gcgcggcaag caggcggcgc
     5581 gccgcctcgc cctcgcccgc caccagcggc tccggcgcgg cgccgcgcgc gccggcggcg
     5641 accaggcggg cgagccagcc ggcgagcagc gccatggcgg tggcgaacgc gtcctcgccg
     5701 ccgcgtcgcg ccaggcggtc gccgaagccg tgcagccggc gcgcgtcgat cccgggcgcg
     5761 ctcgcgatca gcccgaccag atcgcggtat agcgccagcc cgtcgctatc ggccagggcc
     5821 agcgcgcggc ccgggcagcc ctcggcgagg cgcgcaagct gccctgcggc gtcgggggcg
     5881 agcgccggcc gccaccggcg cagcagctcg gcgaccgcat cggcggccag cggcgccaag
     5941 gtcagcatcc ggcagcggct gcgcaaggtg cgcggcaccc gccccggcac gtggcagacg
     6001 agcagcacca gcgcccgccg cggcggctcc tcaaccagct tgagcaccgc gttgaccgcg
     6061 ttgcggttca tctcgtcggc ggcgtcgatc accgccaccc gccagccgcc ctcggccggg
     6121 gtcagggcca ggaactcggc cagccggcgg gcgtcggcga cgaccacctc gctgcgctcg
     6181 cggcccttcg agtcgacgcc gcgctcgatg gtcagcaggt cccgatggcc gcccgaggca
     6241 acgcgccgga acaccggatg gtcggcatcg agcgcaagcc ccgcctcgcc gccgccgacc
     6301 aagtccgcct ggaccgcgcc gccggcgagc acatagcggg cgaaacgata ggcgagcgtc
     6361 gccttgccga ctccgcgcgg cccggcgatc agccaggcgt gcgccagccg ccccgactcg
     6421 aacccgcgcc tgagcgtcgc ctcggcggcg gcgtggccga ccaggtcggg attggcgcgc
     6481 ggcgccggcg gcaggtcgag gtcagtgtcc gccatcgggt acctcaatgc cgagccggtc
     6541 gcggacgacc gcgaacgcct gccgggcgac cgcgtcctcg ggcaggctgg cgtcgatcat
     6601 cacgcagcgg tcgggcgcgc gccgcgcgat gtcgaggaac gccttgcgca ggcgctcgtg
     6661 gaaagcgttg tccatgcgct cgtagcgctg gggggggccg gcgcgggccg ccgcgcgcgc
     6721 cagcccgacg gcgaccggca cgtccaggat cagggtcagg tcgggcgcga agtcgccgac
     6781 cgcgacccgg tacagcgcct cgaccgtctc gcgtcccacg cccatggcgc agccttggta
     6841 agccatggtc gaatcggcga accggtcgca caccacccat ttgcccgccg cgagcgccgg
     6901 ccacacggtc ctgatcaggt gctcacgccg ggcagcgaaa tggagcagcg cctcggcggt
     6961 cgggtcccag cggtcgaggg cgccgtcgac cagcagcgcc cggatcgcct cggcacccgg
     7021 cgcgccgccg ggctcgcggg tggtgatcgc ctcgatcccc gcccgcgcga gactccgggc
     7081 gagatgcgcg cactgggtcg acttgccggc cccctcgccg ccctcgacgg tgaccagcct
     7141 gccccgggtc acgacgtggg cgggccgaaa accaggtagc ggagcgccga caacagccgc
     7201 gcgaccacgc cgagttggcc gacgtcggcg cccgcgacca gcggcacctg caccggttgg
     7261 gcctcgggtg cggtgacggt aagcgtggcc aggcgcgtgc cggcgacgat cggcgccggc
     7321 accggcgcat cggcgaccac cgcgacgcgc atgtcgtcgc ggctgtcgcg cctgagggtg
     7381 acgaccagcg ggtccacgat caccagcggc acccgcgcct cctcgcccag ccacaccggc
     7441 gcctcggcga ccgtctcgcc ggccttgaac agggcgtaat tcctggtgtc gcggaagccc
     7501 cagttgagaa tccgctcggc ctcggccgaa cggtcgctca ccgaaccgag cccggtgatg
     7561 accaggatca ggcggcggcc ctcgcgctcg gcggtcgcgg tcaggctgta gccagcgtcc
     7621 tcggtgtggc cggtcttcat gccgtccgcg ccgagcccct ccttgtacag gagcgggttg
     7681 cggttgccct gcttgatgcc gttgtacgtg aactcggtct cggcgaaaaa gtggaagtaa
     7741 tcggggaagt cccggatcaa gcgctcggtg aggtgggcga ggtcgcgcgg gctcatgtaa
     7801 tggccgggat cgggccagcc gtaagagttg gtgaacgcgc tgttggtcag cccgatctcg
     7861 cgcgcgcgct cggtcatctt ttgcgcgaac gcctgttcgc tgccggccag cgcctcggcg
     7921 accacgacgc aggcgtcgtt gcccgactgg acgatgatgc cttgcagcag gtcgccgacg
     7981 gtgacgttgg tgtcgacctt gacaaacatt ttcgagccgc cggtccgcca cgccttctcg
     8041 ctcaccggca gggggtcgtc gagcgtcagc gttcccgcct tgagccgctc gaacaccatg
     8101 tagatggtca tcagcttgct catcgaggcg ggcggcgccg gggtgtctgc gttcttgtcg
     8161 agcagcaccg cgccggtgtc gaaatcgatc aggatcgcct cgcgcgccgc ggtgtcgaat
     8221 gcctgtgcgg gccatgccag cagcaacaga gggagcagcg caagccacgc ctgcccgcgc
     8281 gaggcccggc accgcgtttt ggggacggtg tggatcatgg tcgactctta ccacctgccg
     8341 cgggcgccga acaacgccgg gcgggccgac gagctgcgcg ctactcggcc tcggcgacca
     8401 gccgcgcgcc gggatggccg gcggcgacca cccggtggag cagggactgg gcggtctccg
     8461 gcgcgtagat cgggccgaga cggacccgat acaggatctg ccggcccgtc tcgaccgcga
     8521 tgacccgggt cggcccgaac ga
//
LOCUS       DASWHU010000188         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_829332, whole genome shotgun sequence.
ACCESSION   DASWHU010000188 DASWHU010000000
VERSION     DASWHU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829332"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGB88_06860"
     CDS             complement(<1..399)
                     /locus_tag="VGB88_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647201.1"
                     /translation="MSVDRGFLLRGLAFWPVLPALAAANGVLRETLLAPALGARAALP
                     LSGVLFVALVLGASAVFLALVRAPRTPGELWRLGALWAGLALAFEVGVFGLIGGAPMR
                     ELLAALDPATGNLFALALLATLAGPRLMAAA"
     gene            complement(396..1292)
                     /locus_tag="VGB88_06865"
     CDS             complement(396..1292)
                     /locus_tag="VGB88_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012490226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9647202.1"
                     /translation="MATEPGRGVAFVDGDWVAVAEARIPLLDWGFLRGDANQDTITVW
                     KGSFFRLDDHLARFDRNIAKLRMTGPYDAAERREIVVECARRAGFSEAYVQMIMTRGR
                     APLGRRDPRTCRNAFYAFCIPYVWIATPEAKRRGLNLHVSERWRIPPESVDPTVKHYH
                     WLDFEMGLFEAYDAGADTVVLSDRDGNVAEGPGFNIFAVQGGKLATPEQGVLDGMTRR
                     TLFELCEEIQLPCRLAAVSRAALAKADEVFLSSSAGGVIPVTRIDGAPVAGGRPGAIT
                     TRLDELYWSKREAGWHATPVTG"
     gene            1402..3210
                     /locus_tag="VGB88_06870"
     CDS             1402..3210
                     /locus_tag="VGB88_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9647203.1"
                     /translation="MARDRDPITFELFKNALFAIADEMALTICRTTYSGVLRDNMDFS
                     TAIADADGRLVAQGLTLPGHLGSIPTALAVVVERFKGDIDPGDAFIMNDPFDGGMHLP
                     DIFIFKPIFAGARRIAFAATICHHGDVGGRVPGSNASDSTEIFQEGLRIPPLKMYAAG
                     QRNDTLSALIEKNVRIPVQVLGDLRAQLAACHIAERELLDLIARHGIEASERYMAEVI
                     DYAERLARAAVRELPDGAWSFEDWIDDDGIEVDTPIRLFCTVRKQGERMSVDWTGTAA
                     QVKGAINNTLSYTKAASYTAVRAILPGDIPNNDGVFRAIEVTAPEGTIANAVLPAACA
                     ARGLTGFRMVDCCLGALAMMVPERVCAASDGGNTGVTIGGWRKDRTPFIFVDFACGTW
                     GGRPWADGLQGNSNMFANMASQSVEVIEAEQPIQILAYELLPDRAGPGKFRGGAPYRR
                     DYRFLEHEAVLQVRADRHKVRPYGLYGGRPGRPSANYLERGGEARPAPSKLTVTITRG
                     DVFRHELAGGGGWGDPLEREPARVLCDVRNGYVSAGSARADYGVVVDTASWAVDEAAS
                     TRLRAELRAARGNAPLPAVSWTDDPHGGSPGGDSRA"
     gene            3207..>4312
                     /locus_tag="VGB88_06875"
     CDS             3207..>4312
                     /locus_tag="VGB88_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9647204.1"
                     /translation="MTGWRLGVDIGGTFTDIVCIDQSGRVLTKKVSSSVGDYARAILD
                     GLAELCAESDLAPGTVAEVVHGTTVGSNAILERKGARTGLITTKGFRDVLEIRNLRMP
                     RLYDLHWDKPPPLVPRYLRLEVAERVNARGEVETPLARADAEAAVDRLVAEGVEVIAV
                     CLLNCFANAAHERLIGAVIEERAPRLAFCLSSDVLPQIKEYERTSTTVINAYVLPVIR
                     GYLAELRARLAGAGVSAPFLLMQSNGGLMSDAAAGARPCMIIESGPAGGVVGALALAR
                     RLGLDDVITFDMGGTTAKASLIEGGRATRSQEYSVGGGIMIGSRLMTGAGYLLKTPAI
                     DLAEVGAGGGSLVWIDPGGSLQVGPESAGADPGPV"
BASE COUNT          627 a         1570 c         1537 g          578 t
ORIGIN      
        1 ggccgccgcc atcagccgcg ggccggccag ggtggcgagg agcgcgagcg cgaacaggtt
       61 gccggtcgcg ggatcgagcg ccgccagcag ttcgcgcatc ggcgcgccgc cgatcagccc
      121 gaagacgccg acctcgaagg caagcgcgag ccccgcccac agcgcgccga gccgccacag
      181 ctcgccgggc gtgcgcggcg cgcgcaccag cgcgaggaac acggccgaag cgcccagcac
      241 cagcgccacg aacaggactc ccgacagcgg cagcgcggcg cgcgcgccga gcgccggcgc
      301 gagcagcgtc tcgcgcagca cgccgttggc cgccgcgagc gccggcagca ccggccagaa
      361 cgcgagcccg cgcagcagga acccccgatc gacgctcacc cggtgaccgg ggtggcgtgc
      421 cagccggcct cgcgcttgga ccagtagagc tcgtcgaggc gcgtggtgat cgcccccggc
      481 cggccgccgg cgaccggggc gccgtcgatg cgcgtgaccg ggatgacgcc gccggcggag
      541 gacgacagga acacctcgtc ggccttggcc agcgccgcgc gcgagacggc cgcgagccgg
      601 cacgggagtt ggatttcctc gcacagctcg aacagggtcc gtcgggtcat gccgtcgagc
      661 acgccctgct ccggcgtcgc gagctttccc ccctgcacgg cgaagatgtt gaagcccggg
      721 ccttcggcca cgttgccgtc gcggtcgctt agcaccaccg tgtcggcgcc ggcgtcgtag
      781 gcctcgaaca gccccatctc gaagtcgagc cagtgatagt gcttgacggt cggatcgacc
      841 gactcgggcg ggatgcgcca gcgctcgctc acgtgcaggt taagcccgcg ccgcttggcc
      901 tcaggcgtcg cgatccagac ataggggatg cagaaggcgt agaacgcgtt ccggcacgtc
      961 cgcgggtcgc ggcggccgag cggcgcacgc ccgcgggtca tgatcatctg cacgtaggcc
     1021 tcgctgaagc cggcgcggcg cgcgcactcg accacgattt cgcggcgttc cgccgcgtcg
     1081 taagggccgg tcatgcgcag cttggcgatg ttgcggtcga agcgcgcgag gtggtcgtcg
     1141 aggcggaaga agctcccttt ccacaccgtg atcgtgtcct ggttggcatc gccgcgcagg
     1201 aaaccccagt cgagcagcgg aatccgcgcc tcggccaccg ccacccagtc gccgtcgacg
     1261 aacgccacgc cccggcccgg ctccgttgcc atgagtcccc tccctatcgg cccgtgtggg
     1321 cgatcttcgg ccacggcgtg cgccttgtca cgcgcgccgg cccaggatta agtggggcgg
     1381 cttggcctga ggggacacga catggcccgc gaccgcgacc cgatcacctt cgagctgttc
     1441 aagaacgcgc tgttcgcgat cgccgacgag atggcgctga ccatctgccg gaccacctat
     1501 tcgggggtct tgcgagacaa catggacttc tcgaccgcga tcgccgacgc ggacgggcgc
     1561 ctggtcgcgc aaggcctgac gcttcccggc catctcggct cgatcccgac cgcgcttgcc
     1621 gtggtggtcg agcgcttcaa gggcgacatc gacccgggcg atgccttcat catgaacgac
     1681 ccgttcgacg gcggcatgca cctgcccgac atcttcatct tcaagccgat cttcgccggc
     1741 gcgcggcgca tcgccttcgc cgccacgatc tgccaccacg gcgacgtcgg cggccgggtg
     1801 ccgggctcga acgcgtccga ctcgaccgag atcttccagg agggcctgcg gataccgccg
     1861 ttgaagatgt acgcggcggg ccagcgcaac gacacgctct cggcgctgat cgagaagaac
     1921 gtgcgcatcc cggtgcaggt gctgggcgat ttgcgcgccc agctcgccgc ctgccacatc
     1981 gccgagcgcg agctgctcga cctgatcgcg cgccacggca tcgaggcgag cgaacgctac
     2041 atggccgagg tgatcgacta cgccgagcgg ctcgcgcgcg ccgccgtgcg cgagctaccc
     2101 gacggcgcct ggagcttcga ggactggatc gacgacgacg gcatcgaggt ggacacgccg
     2161 atccgcctgt tctgcaccgt gcgcaagcag ggcgagcgca tgtcggtcga ctggaccggc
     2221 acggcggcgc aggtcaaggg cgcgatcaac aacacgctgt cctacaccaa ggccgcgtcc
     2281 tacaccgcgg tgcgggcgat cctgcccggc gacatcccca acaacgacgg cgtgttccgg
     2341 gcgatcgagg tgaccgcgcc cgaaggcacc atcgccaacg cggtgctgcc ggcggcgtgc
     2401 gcggcgcgcg ggctgaccgg cttccgcatg gtcgactgct gcctcggcgc gctcgccatg
     2461 atggtgcccg agcgggtgtg cgcggcctcg gacggcggca acaccggggt caccatcggc
     2521 ggctggcgca aggaccgcac gccgttcatc ttcgtcgact tcgcctgcgg cacctggggc
     2581 ggccgcccgt gggccgacgg cctgcaaggc aactccaaca tgttcgccaa catggcctcg
     2641 cagtcggtcg aggtgatcga ggccgagcag ccgatccaga tcctcgccta cgagctgctc
     2701 cccgaccgcg ccggtcccgg caagttccgc ggcggcgcgc cctaccggcg cgactaccgc
     2761 ttcctcgagc acgaggcggt gttgcaggtg cgcgccgacc gccacaaggt ccgcccctac
     2821 ggcctctatg gcggccggcc cgggcggccg agcgccaact acctcgagcg gggcggcgaa
     2881 gcccggccgg cgccgtcgaa gctcaccgtc accatcaccc gcggcgacgt gttccgccac
     2941 gagctcgccg gcggcggcgg ctggggcgac ccgttggagc gcgagccggc gcgcgtgctc
     3001 tgtgacgtgc gcaacggcta tgtcagcgca ggctccgcgc gcgccgacta cggcgtcgtc
     3061 gtcgacaccg cgagctgggc ggtcgacgag gccgcgagca cacgcctgcg cgccgagctg
     3121 cgcgccgcgc gcggcaacgc gccgctgccc gcggtgagct ggacggacga tccgcacggc
     3181 ggcagccccg gcggcgacag ccgcgcatga ccggctggcg cctcggcgtc gacatcggcg
     3241 gcaccttcac cgacatcgtc tgcatcgacc agtcggggcg ggtgctgacc aagaaggtct
     3301 cctccagcgt cggcgactac gcgcgcgcga tcctggacgg gctcgccgag ctgtgcgccg
     3361 agagcgacct tgcgccgggc acggtggccg aagtagtgca tggcaccact gtcggctcga
     3421 acgcgatcct cgagcggaag ggcgcgcgca cgggcctgat caccaccaag ggcttccgcg
     3481 acgtgctcga gattcgcaac ctgcgcatgc cccggctcta cgacctgcac tgggacaagc
     3541 cgccgccgct ggtgccgcgc tacctgcgcc tcgaggtggc ggagcgcgtg aatgcccgcg
     3601 gcgaggtcga gacgccgctt gcgcgcgccg acgccgaggc cgcggtcgac cggctggtcg
     3661 ccgagggcgt cgaggtgatc gccgtatgcc tgctcaactg cttcgcgaat gcggcgcacg
     3721 agcgcctgat cggcgcggtg atcgaggagc gcgcgccgcg cctcgccttt tgcctgtcgt
     3781 ctgacgtgct gccgcagatc aaggagtacg agcgcacctc gaccacggtg atcaacgcct
     3841 atgtgctgcc ggtgatccgc ggctatctgg ccgagctgcg cgcgcgcctc gccggcgccg
     3901 gcgtctccgc gccgtttttg ctgatgcagt cgaacggcgg gctgatgagc gatgcggcgg
     3961 cgggcgcgcg cccgtgcatg atcatcgagt ccggccccgc cggcggggtg gtcggcgcgc
     4021 tggcgctcgc gcgaaggctg gggctcgacg acgtcatcac cttcgacatg ggcggcacca
     4081 ccgccaaggc ctccctcata gagggtgggc gcgccacgcg gagccaggag tactcggtcg
     4141 gcggcggcat catgatcggc tcgcgcctca tgaccggcgc cgggtatctc ttgaagacgc
     4201 cggcgatcga cctcgccgag gtcggcgcgg gcggcggctc gctggtgtgg atcgatccgg
     4261 gcggctcgct gcaggtcggg cccgaaagcg cgggcgccga ccccgggccg gt
//
LOCUS       DASWHU010000189         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_829788, whole genome shotgun sequence.
ACCESSION   DASWHU010000189 DASWHU010000000
VERSION     DASWHU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829788"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1404)
                     /gene="hisD"
                     /locus_tag="VGB88_06880"
     CDS             complement(103..1404)
                     /gene="hisD"
                     /locus_tag="VGB88_06880"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9647205.1"
                     /translation="MARRLDSGAPGFARAFAELLGTKRESTPEVGEAVAAIIAEVRRR
                     GDAALIDYTERFDRIRLDAAKLRFTAAEIDAARAACSAEVLAALDQAAARIEAYHRRQ
                     LPQDEMYRDDAGVELGVRWRPVDSAGLYVPGGTAAYPSTVLMNAIPARVAGVKRVVMT
                     TPTPDGAHNPLVLAAAATAGVAEVYRVGGAQAIAALAFGTESIARVDKVVGPGNAYVA
                     AAKRQVFGVVGIDLIAGPSEVLVVADAGNDARWIALDLLAQAEHDTAAQSILITDDGG
                     FADAVAQAVERELATLPRAEIAAASWRDHGVIVVVGDLLAEAPALIDRVAPEHLQLAL
                     AEADALAARVTHAGAIFLGRLTPEAIGDYVGGPNHVLPTARSARFSSGLSVLDFVKRT
                     SLLRCDSASLAALGPAAVTLAGAEGLDAHARSVSVRLDGGD"
BASE COUNT          196 a          566 c          577 g          193 t
ORIGIN      
        1 tcgatctcgg ccgaccagcc caccacgctg ccggcgtcga gggtcagctc gacgatgcgc
       61 tggctgtcgg gcggttccgc gtcggagccc atgcgcccgg gatcaatcgc ccccgtccag
      121 gcggaccgag accgagcgcg cgtgcgcatc gagcccctcg gcgccggcca gcgtcaccgc
      181 cgccggcccg agcgcggcga ggcttgccga gtcgcagcgc agcaggctcg tgcgcttgac
      241 gaagtcgagc accgacaggc ccgacgagaa gcgggcgctg cgcgctgtcg gcagcacgtg
      301 gttgggcccg ccgacatagt cgccgatcgc ctcgggcgtg agccggccga ggaagatcgc
      361 gccggcatgg gtgacgcggg cggcgagcgc gtcggcttcg gcgagcgcga gctggaggtg
      421 ctcgggcgcg acgcggtcga tcagtgccgg cgcctcggcg agcaagtcgc cgaccaccac
      481 gatcacgccg tggtcgcgcc agctcgccgc cgcgatttcg gcccgcggca gggtcgcgag
      541 ctcgcgctcg accgcttgcg cgaccgcatc ggcgaacccg ccgtcgtcgg tgatcaggat
      601 cgactgggcg gcggtgtcgt gctcggcctg ggccagcagg tcgagggcga tccagcgcgc
      661 atcgttgccg gcgtcggcga ccaccagcac ctcggacggg ccggcgatca ggtcgatccc
      721 gaccacgccg aacacctggc gcttggcggc ggcgacgtag gcgttgccgg ggccgaccac
      781 cttatcgacg cgggcgatgc tctcggtgcc gaaggccagc gccgcgatcg cctgggcgcc
      841 gccgacgcga tagacctcag cgacgccggc ggtggcggcg gcggcgagca ccagcgggtt
      901 gtgcgcgccg tcgggagtcg gcgtggtcat gaccacgcgc ttgacgccgg caacgcgcgc
      961 cgggatcgcg ttcatcagca ccgtgctcgg ataggccgcg gtgccgcccg gcacgtacag
     1021 gccggccgag tcgaccgggc gccagcgcac gccgagctcg accccggcgt cgtcgcggta
     1081 catctcgtcc tgcggcagct ggcggcgatg gtaggcctcg atgcgcgccg ccgcctggtc
     1141 gagcgcggcc agcacctcgg cgctgcacgc cgcgcgcgcg gcgtcgatct cggcggcggt
     1201 gaaccgcagc ttggctgcat cgagccggat gcggtcgaag cgctcggtat agtcgatcag
     1261 cgccgcatcg ccccgccggc gcacctcggc gatgatcgcg gcgaccgcct cgccgacctc
     1321 gggcgtgctc tcgcgcttgg tgccgagcag ctcggcgaag gcgcgcgcga agcccggcgc
     1381 gccgctgtca agccgccgcg ccatgcacca cctcgcgcag ccggtcgagc cagcggccga
     1441 ggtcccccgg gcgggtcttc caggcggtcc ggttgacgat caggcgcgcc gagacgtcgg
     1501 cgatcttctc gacctcgacc aggccgttgg cc
//
LOCUS       DASWHU010000190         5931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_830005, whole genome shotgun sequence.
ACCESSION   DASWHU010000190 DASWHU010000000
VERSION     DASWHU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830005"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGB88_06885"
     CDS             <1..429
                     /locus_tag="VGB88_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEX9647206.1"
                     /translation="ARAVGMPARLGYGDVRNHLATAKLLETMGTDLFVWHGYVELWID
                     GRWVKATPAFNRELCARFGVRSLEFDGRSDSLFHPFDVTGRKHMEYVRQHGHFTDLPY
                     DRIMAAWREHYPQAAVASGFAAGGDFAADAAAERTGAGAA"
     gene            438..1463
                     /gene="dctP"
                     /locus_tag="VGB88_06890"
     CDS             438..1463
                     /gene="dctP"
                     /locus_tag="VGB88_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9647207.1"
                     /translation="MPAARLAVLAGALVALTWAAPGAEAAPRVLRATIQLPLTSPLGA
                     NLVAFKERVEAASAGAIKVELYPSAQLFTDREVPAAVASGQIEMGVASLARFAGTIPA
                     VDIFNVPFLLNTEALVRAATEPGSPVRDPLDQAMTAIGARPLWWQPYGLSVMMLRGTE
                     PPRLPADLKGLKIRTYGKALEGFVNALGAAATNVSGERQYLAYERGMVDGGMTGLFSV
                     KERKIYQVLHSLTFTNHSDIEFVVVINERLWQQLDDGERELLSQAARAVEVELRDDFA
                     ALEAEALEEAIAGGMAVHRLSEDELAAWRRATAPLEQAYLDEAGPLGVELVAAARQLR
                     AELGADD"
     gene            complement(1532..2434)
                     /locus_tag="VGB88_06895"
     CDS             complement(1532..2434)
                     /locus_tag="VGB88_06895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9647208.1"
                     /translation="MTTTESQQFADQGHLRAVLNTMLDGLVITDDRGIVQVFNPACER
                     LFGYRAEDVLGRDVSLLVAEPHRRKHREYVDSYRTTGEPNIIGRQREVEAQRHDGTVF
                     PVSISIGEAEVDGRRFFIGVVHDLTQEVLLRRDATIDPLTGALNRRDFLAKGTAEVTR
                     ARRYGRVCTLLMLDIDHFKVVNDRYGHAAGDEALRRFTAACKEILRENDIFGRIGGEE
                     FAVILPETKLSGARKLAERLRRRTAEIEIANSGQRFTFTVSIGVAQLAKSDNDIDKFL
                     KRADRALYAAKERGRDRVVVADAA"
     gene            2653..3192
                     /locus_tag="VGB88_06900"
     CDS             2653..3192
                     /locus_tag="VGB88_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9647209.1"
                     /translation="MAARPAILSAAVRLVDGLSRLCAWAAAAAFFVIGVIVAYEVAMR
                     YLFAAPTRWVEETASVLQIYGVFLAAAWLVGTRAHIRISVVTARLPAPARLWTARAGL
                     VAVAAVAAVSAWFTVGLIEFSLATGQYTDSTLALPMWLLQAPVAGGLALAALQALATV
                     AASFAEPERLLAEADMGGA"
     gene            3197..4486
                     /locus_tag="VGB88_06905"
     CDS             3197..4486
                     /locus_tag="VGB88_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9647210.1"
                     /translation="MAGALVLAAMFAALLAGIDVAFALAGLGVVLLLLADFSPLMIPQ
                     ALMSSMDNFILLSVPLFILMSNILLRGGAGEDLFGAVQSWVGHWPGGLAVATVLSCAI
                     FASISGSSVATSATIGTVAIPAMVTRGYQRRFTYGLLAAGGTLGILIPPSIPMIIYGF
                     VTGESIIELFLAGIGPGVLLTLMFIAYAVVRARLKRDYVATARADARQRIAASRRALP
                     ALALAVLIVAGIYFGAFTPTEAAAIGFAGALVITMVVNRTLRPADLAPIALDSMRTSV
                     MVLFIIAGAKVFGQAITLYQIPQDVTAAVVGGLATPGAFIVVICALLLAMGCVLETLS
                     MMLLMLPVIQPSLIGLNIDAIWFGVIFTMMIECALITPPVGLNLFVIRAVAAQGGRVV
                     TVADVVYGTLPFVVLILIAVAITTALPDLALFIPRSL"
     gene            4527..5438
                     /locus_tag="VGB88_06910"
     CDS             4527..5438
                     /locus_tag="VGB88_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9647211.1"
                     /translation="MFQAPPEIATRVFSRLPDSFRRPGTRTAWAEVMTQGGTVDSFLE
                     GPSFDRDGNLYVVDIPYGRVFRIDPAGGWTLVCEYDGEPNGLKFHADGRAFIADHKHG
                     ILQLDAENGTVSPVIDRPRHERFKGCNDLFFAADGTLYFTDQGQTGLHDATGRLYRLT
                     PDGRLDCLLDAVPSPNGLVTNLTETMLYLAVTRANAVWRVPMLADGRPTKVGTFVQMS
                     GGLAGPDGMALDEDGNLAVAHAGLGAVWLFSRLGEPLARVRSSAGLMTTNLAYGGADR
                     RTLYITESETATVLAADMPVPGRRMYG"
     gene            complement(5435..>5931)
                     /locus_tag="VGB88_06915"
     CDS             complement(5435..>5931)
                     /locus_tag="VGB88_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647212.1"
                     /translation="LAYFLAVHHPERLHSIVACTAPHRGAAVDWLGEWRAFVEANGMC
                     AWSERMMERRFAPGALDDATWQWFHRTQAACAAHVVLDLGDMLAQVDLSAELGRVATP
                     CLMIAGDSSPFLPPPVLADTHARVPGAEMRLFNRARHGVVLSHGREAAEVARDFLARV
                     DRSR"
BASE COUNT          767 a         2111 c         2112 g          941 t
ORIGIN      
        1 gcgcgcgcgg tcggcatgcc ggcgcggctc ggctatggcg acgtgcgcaa ccacctggcg
       61 accgccaagc tgctcgagac catgggcacc gacctgttcg tctggcacgg ctatgtcgag
      121 ctttggatcg acggccgctg ggtcaaggcg acgccggcgt tcaatcgcga gctgtgcgcg
      181 cgcttcgggg tgaggtcctt ggagttcgac ggccgcagcg actcgctgtt ccacccgttc
      241 gacgtgaccg ggcgcaagca catggagtat gtccgccagc acggccactt caccgacctg
      301 ccttacgacc ggatcatggc cgcgtggcgg gagcactacc cgcaggctgc ggtggcaagc
      361 ggattcgccg cgggcggcga cttcgcggcc gacgccgcgg ccgagcggac cggggccggc
      421 gcggcctgac ggcggccatg cccgcggcgc ggcttgccgt cctcgccggc gccctggtcg
      481 cgctgacctg ggccgcgccg ggcgccgagg cggcgccgcg ggtgctgcgg gcgaccatcc
      541 agctgccgct gacctcgccc ttgggcgcca acctggtcgc gttcaaggag cgggtcgagg
      601 cggcgagcgc cggcgcgatc aaggtcgagc tctaccccag cgcccagctg ttcaccgacc
      661 gcgaggtgcc ggccgcggtc gcctcgggcc agatcgagat gggtgtcgcc tcgctcgccc
      721 gcttcgccgg caccatcccg gcggtcgaca tcttcaacgt gccgttcctg ctcaacaccg
      781 aggcgctggt gcgtgcggcg accgagccgg gctcgccggt gcgcgacccg ctcgatcagg
      841 cgatgaccgc gatcggcgcc cgcccgctgt ggtggcagcc gtacgggctg tcggtgatga
      901 tgctgcgcgg gaccgagccg ccgcgcctgc ccgcggacct caaggggctc aagatccgca
      961 cctacggcaa ggcgctggag gggtttgtca atgcgctcgg cgccgccgcc accaacgtct
     1021 cgggcgagcg ccagtacctg gcctacgagc gcggcatggt ggacggcggc atgaccggcc
     1081 tgttcagcgt caaggagcgc aagatctacc aggtgctgca ctcgctgacc ttcaccaacc
     1141 actcggacat cgagttcgtg gtggtgatca acgagcgcct gtggcagcag ctcgacgacg
     1201 gcgagcggga gcttctgagc caggccgcgc gcgcggtcga ggtcgagctg cgcgacgact
     1261 tcgcggcgct cgaggccgag gcgctggagg aggcgatcgc gggcggcatg gccgtccacc
     1321 ggctgagcga ggacgagctc gccgcctggc gcagggcgac tgcgccgctc gagcaggcct
     1381 atctcgacga ggccgggccg ctcggcgtcg agctggtggc ggcggcgcgc caactcaggg
     1441 ccgagctcgg cgccgacgac tgagaggctg tgaagaaatc accggcgggt cccgtcatcg
     1501 tcgttcccgc gaaagagcgg cgaggggagg ctcaagcggc gtcggcgacc accacccggt
     1561 cgcggccgcg ctccttggcc gcatacagcg cgcggtcggc gcgcttgagg aacttgtcga
     1621 tgtcgttgtc ggacttggcc agctgggcga cgccgatgct gaccgtgaag gtgaaccgct
     1681 ggccgctgtt cgcgatctcg atctcggcgg tgcgccggcg caggcgctcg gccagcttgc
     1741 gcgccccgga cagcttggtc tcgggcagga tgaccgcgaa ctcctcgccg ccgatccgcc
     1801 cgaagatgtc gttctcgcgc aggatctcct tgcaggcggc ggtgaagcgc cggagcgcct
     1861 cgtcgcccgc cgcatgaccg tagcggtcgt tgaccacctt gaagtggtcg atgtcgagca
     1921 tcaacagcgt gcagacgcgg ccatagcgcc gggcgcgggt gacctcggcg gtgcccttgg
     1981 ccaggaagtc gcgccggttg agcgccccgg tcagcgggtc gatggtggcg tcgcggcgca
     2041 gcagcacctc ctgggtcagg tcgtggacga cgccgatgaa gaagcgccgg ccgtcgacct
     2101 cggcctcgcc gatcgagatc gacaccggga acacggtgcc gtcgtggcgc tgcgcctcga
     2161 cctcgcgctg gcggccgatg atgttcggct cgccggtggt gcggtagctg tcgacgtact
     2221 cgcggtgctt gcgccggtgc ggctccgcga ccagcaggct cacgtcccgg ccgagcacgt
     2281 cctcggcacg gtagccgaac agccgctcgc aggccggatt gaacacctgg acgatgccgc
     2341 ggtcgtcggt gatcaccagc ccgtcgagca tggtgttcaa gaccgcgcgc agatggccct
     2401 gatcggcgaa ctgctgtgat tcggtggtcg tcatgtcgct ggtcccgagg cgtccggccc
     2461 cttggccggc gcccgcgccg tccgcgtcag ccctgagact gtcagctggg tcggacgtgc
     2521 cctatactcc accccgctcc agtcaggagt cgcaccccgg cccggctggc cggcgctcgc
     2581 tccgcatatc ggtcacatcc ccctgatgcg cgcccctatt tctagcctta ccgccttgcc
     2641 cggacaagcg tcatggccgc ccgcccggcg attttaagcg ccgccgtacg cctggtcgac
     2701 gggctcagtc ggctgtgcgc gtgggcggcg gcggcggcgt tcttcgtcat cggcgtgatc
     2761 gtcgcctacg aggtggcgat gcgctacctg ttcgcggcgc cgacccgctg ggtcgaggag
     2821 accgcgagcg tgctccagat ctacggcgtg ttcctggccg ccgcctggct ggtcggcacc
     2881 cgcgcgcaca tccgcatctc ggtggtcacc gcgcggctgc ccgcgccggc ccggctgtgg
     2941 acggcgcgcg ccggcctggt ggcggtggcc gcggtggcgg cggtctcggc gtggttcacg
     3001 gtcgggctga tcgagttctc gctggcgacc ggccagtaca ccgactccac gcttgcgctg
     3061 ccgatgtggc tgttgcaggc gccggtcgcg ggcggccttg cgcttgccgc gctgcaggcg
     3121 ctggcgaccg tcgcggcgag cttcgccgag cccgagcggc tgctcgccga ggccgacatg
     3181 ggcggggcct gagggggtgg ccggggcgct ggtactggcc gcgatgttcg cggcgctgct
     3241 ggccggcatc gacgtcgcct tcgcgcttgc cgggctcggc gtggtgctgt tgctgctcgc
     3301 cgacttctcg ccgctgatga tcccgcaggc gctgatgtcg agcatggaca acttcatcct
     3361 gctgtcggtg ccgctgttca tattgatgtc caacatcctg ctgcgcggcg gcgccggcga
     3421 ggacctgttc ggcgccgtgc aaagctgggt cggccactgg ccgggggggc tggcggtggc
     3481 gaccgtgctg tcatgcgcga tcttcgcctc gatctcgggc tcgtcggtgg cgacctcggc
     3541 gaccatcggc acggtggcga tcccggccat ggtcacgcgc ggctatcagc gccggttcac
     3601 ttacggcctg ctcgccgccg gcggcacgct cggcatcctg atcccgccgt cgatcccgat
     3661 gatcatctac ggtttcgtca ccggcgagtc gatcatcgag ctgttcctgg ccgggatcgg
     3721 accgggtgtc ctgctcacgc tcatgttcat cgcctatgcc gtcgtccgtg cccggctcaa
     3781 gcgggactac gtagcgacgg cgcgcgcgga tgcccgccag cgcatcgccg ccagccgccg
     3841 cgcgctgccc gcgctcgcgc ttgccgtgct gatcgtcgcc gggatctatt tcggcgcctt
     3901 caccccgacc gaggcggcgg cgatcggctt tgccggcgcg ctggtgatca ccatggtggt
     3961 gaaccgcacg ctcaggccgg ccgacctggc gccgatcgcg ctcgactcga tgcgcacctc
     4021 ggtgatggtg ctgttcatca tcgccggcgc caaggtgttc ggccaggcga tcacgctcta
     4081 ccagatcccc caggacgtga ccgcggcggt ggtcggcggc ctggccactc cgggcgcgtt
     4141 catcgtcgtc atctgcgcgc tgctgctggc gatgggctgc gtgctcgaga cgctgtcgat
     4201 gatgctgctg atgctgccgg tgatccagcc gtcgctgatc ggcctcaaca tcgacgcgat
     4261 ctggttcgga gtcatcttca ccatgatgat cgaatgcgcg ctgatcaccc cgccggtggg
     4321 cctgaacctg ttcgtgatcc gcgcggtcgc ggcccagggc gggcgcgtcg tgaccgtcgc
     4381 cgacgtggtc tacggcacgc ttccgttcgt ggtcctgatc ctgatcgcgg tggcgataac
     4441 gaccgccttg cccgatctcg ccctgttcat cccccgtagc ctttgatcgc ccgccgccag
     4501 ccggagcccc agccggagac caagccatgt tccaagcccc gcccgagatc gcgacccgcg
     4561 tgttctcgcg cctgcccgac tcgttccgcc gccccggcac gcgcaccgcc tgggccgagg
     4621 tgatgacgca gggcggcacc gtcgacagct tcctcgaggg cccgtcgttc gaccgcgacg
     4681 gcaacctgta cgtggtcgac atcccctacg ggcgcgtctt ccgcatcgat cccgccggcg
     4741 gctggacgct ggtgtgcgag tacgacggcg agcccaacgg cctcaagttc cacgccgacg
     4801 ggcgcgcctt catcgccgac cacaagcacg gcatcctgca gctggatgcg gaaaacggca
     4861 cggtcagccc ggtcatcgac cggccccggc acgagcgctt caagggctgc aacgacctgt
     4921 tcttcgccgc cgacggcacg ctctacttca ccgaccaggg ccagaccggc ctccacgatg
     4981 cgaccggccg gctgtatcgc ctcacgccgg acggcaggct cgactgcctg ctcgatgccg
     5041 tgccgagccc aaacggcctg gtgaccaacc tcaccgagac catgctctac ctcgcggtca
     5101 cccgcgccaa tgcggtctgg cgggtgccga tgctggccga cggccggccg accaaggtcg
     5161 gcaccttcgt gcagatgtcg ggcggcctcg ccgggcccga cggcatggcg ctcgacgagg
     5221 acggcaacct tgcggtcgcc catgccgggc tcggcgcggt gtggctgttc agccgcttgg
     5281 gcgagccgct ggcgcgggtg cgcagctcgg ccgggctgat gaccaccaac ctcgcctatg
     5341 gcggggcgga ccggcgcacg ctctacatca ccgagtccga gaccgccacc gtgctcgccg
     5401 ccgacatgcc ggtgccgggc cggcgcatgt acggctaacg gctgcggtct acgcgcgcga
     5461 ggaagtcgcg cgcgacctcg gcggcctcgc gcccgtggct cagcaccacg ccgtggcggg
     5521 cgcggttgaa cagccgcatc tcggcgccgg gcacacgtgc gtgggtatcg gcgagcaccg
     5581 gcggcggcag gaacgggctc gaatcgcccg cgatcatcag gcatggcgtg gcgacgcggc
     5641 cgagctccgc cgacaggtcg acttgggcca gcatgtcgcc caggtcgagc accacatggg
     5701 cggcgcacgc cgcctgggtg cggtggaacc actgccaggt ggcgtcgtcg agcgcgcccg
     5761 gggcgaagcg ccgctccatc atccgctccg accacgcgca catgccgttg gcctcgacga
     5821 aggcgcgcca ttcgcccagc cagtcgaccg cggcgccgcg atggggcgcg gtgcaggcga
     5881 cgatgctgtg cagccgctcc gggtggtgga cggcgaggaa gtaggcgagc g
//
LOCUS       DASWHU010000191        10009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3060888, whole genome shotgun sequence.
ACCESSION   DASWHU010000191 DASWHU010000000
VERSION     DASWHU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060888"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGB88_06920"
     CDS             <1..714
                     /locus_tag="VGB88_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX9647213.1"
                     /translation="ITDYRQRQRRELRGAPMARSSLEQMMNQLREAGRKEIPIVVKAD
                     VQGSAEAIIGSVDKIGTEEVGARVVHGGVGGITESDISLATASGAIVIGFNVRANKQA
                     REAAEQAGIEIRYYNVIYDLVDDMRQALSGMLAPTIREHFLGNAEILEVFNVSKIGKV
                     AGCRVTEGNVRRGSRVRLIRDNVVIHDGKLSTLKRFKDEVREVTAGQECGMGFENYQD
                     IKQGDVIECFDVEEVARSL"
     gene            766..1164
                     /gene="rbfA"
                     /locus_tag="VGB88_06925"
     CDS             766..1164
                     /gene="rbfA"
                     /locus_tag="VGB88_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018304327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9647214.1"
                     /translation="MTSKSGRPRSQRQLRVGELLRHALSEIIGRGDIRDPALDGAAVT
                     VLEVSVSPDLRHAIAYVMPLGGMRKDEILQALRRSRRFVRGQLAREIKLKFMPDVDFR
                     LDTTFDYSDSVQRLLHDPRVARDLVDPDPE"
     gene            1182..2108
                     /gene="truB"
                     /locus_tag="VGB88_06930"
     CDS             1182..2108
                     /gene="truB"
                     /locus_tag="VGB88_06930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9647215.1"
                     /translation="MARRKTGRPVHGWVILDKPVGMTSTRAVSAVRRIFDARKAGHGG
                     TLDPLATGLLPIALGEATKTVSFVMDGAKTYRFTVRWGIGTTTDDTEGEPTATSDVRP
                     SRAAIEAVLPRFAGTISQVPPRFSAVKVAGERAYDLARDGGEPELDAREVRIDRLDLV
                     EMAGADETVFEADCGKGTYVRSLARDLGRALGTFAHVTALRRTRVAGFGEDDMISLEK
                     LEELSHIAAGQETLADVLCSMETALDDIPALAVSGADAARLMRGQAVILRGRDAPILH
                     GTVLVASGGNPVALAEMKQGALHPKRVFNLAG"
     gene            2137..2406
                     /gene="rpsO"
                     /locus_tag="VGB88_06935"
     CDS             2137..2406
                     /gene="rpsO"
                     /locus_tag="VGB88_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9647216.1"
                     /translation="MSITAERKSALIKEFATKDGDTGSPEVQVAILTERIKNLTDHFK
                     GHAKDNHSRRGLLKMVSQRRSLLDYVKSRNEERYQDLIKRLGIRR"
     gene            2566..4683
                     /gene="pnp"
                     /locus_tag="VGB88_06940"
     CDS             2566..4683
                     /gene="pnp"
                     /locus_tag="VGB88_06940"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9647217.1"
                     /translation="MFKIHREEIMWGGRPLVLETGRIARQADGAVLVTYGETTVLATA
                     VADRQPKPGLDFFPLTVNYQEKTYAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FADGFRNETQIICTVLSHDLENDPDIPAMVGASAALTISGIPFLGPIGAARVAYVDGV
                     LVANPTHAELQASELDLVVAGTASGVLMVESEARELAEEVMLGAVMFGHREMQPVVGA
                     IIELAERCAKEPWDLPSKPDLAPTRRRVEEIARAELEQAYGTVVKQDRVQRLDAVRGR
                     IADAFAADEAEAHTAGAVLKSLEKDIVRGAVLATGRRIDGRDTRTVRPIRAEVGVLPL
                     AHGSALFTRGETQALVVTTLGTGQDEQIVDALEGEYRQHFMLHYNFPPYSVGEASFLR
                     GPGRREIGHGKLAWRAVNPLMPAKESFPYTVRVVSEITESNGSSSMATVCGASLSLMD
                     AGVPMKAPIAGIAMGLIKDGDKHVVLSDILGDEDHLGDMDFKVAGSAAGVTSLQMDIK
                     ITGITEAIMRAALDQAREGRLHILDEMAKALSRSRDQVSQNAPRITTLTIPRDKIREV
                     IGTGGKVIREITETTGTKIDIEDDGTIKVAATTSEAGEAAVKWIRDIVAEPEVGVVYD
                     GTVVKVVDFGAFVNFIGNRDGLVHISELAPRRVKTVGEVVKEGDKVKVKVLAIDDRGK
                     VRLSMKVVDQHTGAAVTDEAVPG"
     gene            complement(4723..5427)
                     /locus_tag="VGB88_06945"
     CDS             complement(4723..5427)
                     /locus_tag="VGB88_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX9647218.1"
                     /translation="MEPTTDQILDALCADPTSARERLLHTAGMMFAERGPDAVSTREL
                     TKTAGANLSAIAYYFGGKDGLYNEAVDYTIADTRKLIDGAEQRLRAELAAAGADRAKL
                     ARAASAFVCAVLTALLGRGPEQWPWRLVLREIDHPTGAFERLYQAIFAPFTDAFADLV
                     VAATGREPGEEETVILTNALLGECLIFLRNRPLVLRNLGWSEYTPRRIALVVEIVVDG
                     ILDALDLPRAGAGNGG"
     gene            5589..6986
                     /locus_tag="VGB88_06950"
     CDS             5589..6986
                     /locus_tag="VGB88_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9647219.1"
                     /translation="MKALSPIVLSGRDVMPLVEGGKGISVSNGQSSGAWAAAGAVGTF
                     SGVNADALDEAGNLIPVVYHGRTRRERHEELIAYAIRGGITQARIAHEQSCGEGRIHM
                     NVLWEMGGSERVLEGVLTGARGLIHGVTCGAGMPYRIAEISARHGVYYYPIVSSARAF
                     RALWKRAYHKFPEWLGGVVYEDPWLAGGHNGITNSEDPQTPEDPYPRVLALRRMMCEF
                     GIGATPIFMAGGVWYLREWDDWIDNPELGPIAFQFGTRPLLTQESPISDAWKQRLRDL
                     KPGDISLNRFSPTGFWSSAVNNEFLAELRARAARQVAYTTEQVGEHRIPFEIGPRKRI
                     AYLTEPDAEHAAQWYAEGFTEGLRTPDSTLVFVTPEKAAEIRRDQVECMGCLSACNFS
                     NWAQNDHHTTGRKTDPRSYCIQKTLQDIAHGGDIRTQLMFAGHGAYRFATDPFYSNGF
                     IPTVRELIERLQTGH"
     gene            7067..7501
                     /locus_tag="VGB88_06955"
     CDS             7067..7501
                     /locus_tag="VGB88_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9647220.1"
                     /translation="MTRPHADPDLASLLRAAGLRPTSQRRALARLLIEGGPRHLTAEL
                     LHAEARARKLKVSLATVYNTLHQFTEAGLVRELVVDAGRAYFDTNTSHHHHFYDESDG
                     RLIDIPGDSVAVSALPVPPPGNEISRVDVVVRLRRKRAQPAG"
     gene            7639..8058
                     /locus_tag="VGB88_06960"
     CDS             7639..8058
                     /locus_tag="VGB88_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX9647221.1"
                     /translation="MAKLAGSKTEQNLKDAFAGESMANRRYLFFAQKADVEGFNDVSA
                     VFRSTAEGETGHAFGHLEYLIEVGDPATGKAIGDTSKNLKAAIAGETHEYTDMYPGMA
                     KTARTEGFEEIADWFETLAKAERSHAGRFQKALAAMD"
     gene            8167..9510
                     /locus_tag="VGB88_06965"
     CDS             8167..9510
                     /locus_tag="VGB88_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX9647222.1"
                     /translation="MEGGLNAPVRHPIRYRDPDFHDADKLDAELRRVFDVCHGCRRCF
                     NLCDSFPRLFDLIDSSETGELDSVDSADFARVVGACTLCDMCFMTSCPYVPPHEFDID
                     FPHLMLRYRSAELAKGKLGVIDRELTKTDRNGRLAGAVAPLANWTTDTRNRLTRPALE
                     ALAGIHRDAALPRFHARSFAKRAKQAPPAVNAAAPAHGRKVALFSTCFANYNNPDIAM
                     AALKVLAHNGVEVEVAYPGCCGMPQLEHGDFAEVAQRATRIAPMLGAWIDRGYDIVAP
                     VPSCALMLKFEWPLVVPGDAAVKRLAEHTADITQYVVDIARTEGLADGLEPLDGGVAV
                     HLACHARAQNMGAKAVEMLRLIPDIELEVIERCSGHGGSLGIKTEFFEVALKVGRPAA
                     RKALQAGKAYLASECPLAAEHLRQGIERLAEDAPVPAHAWHPIELLARAYGLADE"
     gene            9516..>10009
                     /locus_tag="VGB88_06970"
     CDS             9516..>10009
                     /locus_tag="VGB88_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX9647223.1"
                     /translation="MARHKLARADILPMAEFAKVRRDRRRALAEVKRHRRVAVGPHAT
                     FYFENRDTIWMQVHEMLFIERGGEAQIAGELEAYNPLIPNGRELVATVMFEIPDVALR
                     RRVLAALGGVEETIELTVAGQAIKGEAEREIDRTTAEGKASAVHFVHFPFTPAQIAAF
                     RDPKA"
BASE COUNT         1655 a         3541 c         3419 g         1394 t
ORIGIN      
        1 attaccgact atcgtcagcg ccagcggcgc gagttgcgcg gcgcaccaat ggcgcgcagt
       61 tccctcgagc agatgatgaa ccagctgcgc gaggccggcc gcaaggaaat cccgatcgtc
      121 gtcaaggccg acgtccaggg ctcggccgag gcgatcatcg gctcggtcga caagatcggc
      181 accgaggaag tcggcgcccg ggtcgtccat ggcggcgtcg gcggcatcac cgagtccgac
      241 atctcgctgg cgacggcctc tggcgccatc gtcatcggct tcaacgtccg cgccaacaag
      301 caggcccgcg aggccgccga gcaggccggc atcgagatcc gctactacaa cgtcatctac
      361 gacctggtcg atgacatgag gcaggccctt tcgggcatgc tggcgccgac catccgcgag
      421 catttcctcg gcaatgcgga gatcctggag gtgttcaacg tctccaagat cggcaaggtc
      481 gccggctgcc gcgtcaccga gggcaacgtc cggcgcgggt cgcgcgttcg cctgatccgc
      541 gacaacgtgg tcatccacga cggcaagctg tcgaccctga agcgcttcaa ggacgaggtc
      601 cgcgaggtga ccgccggcca ggaatgcggc atgggcttcg agaactatca ggacatcaag
      661 cagggcgacg tgatcgagtg tttcgacgtc gaggaagtcg cccgcagcct ttagcgcgcg
      721 gcgggagccg gccagtgccg ggcaccccgc gcctgaagag ggaccatgac atcgaaaagc
      781 ggtcgcccgc ggagccagcg ccagctgcgc gtcggcgagc tcctccgaca tgccctgtcc
      841 gagatcatcg ggcgcggcga catccgcgat ccggcgctgg acggcgccgc ggtcaccgtg
      901 ctcgaggtct cggtcagccc cgacctcagg catgcgatcg cctacgtcat gcccctgggc
      961 ggcatgcgca aggacgagat cctgcaggcg ctcaggcgct cgcgccgttt cgtccgcggc
     1021 cagctcgccc gcgagatcaa gctgaagttc atgcctgacg tcgacttccg gctcgacacc
     1081 acgttcgact attccgattc cgtccagcgc ctgctccacg acccgcgggt tgcccgcgac
     1141 ctcgtggacc cggacccaga ataacccgga gcgacacgac catggcgcgc aggaagaccg
     1201 gccgtcccgt ccacggctgg gtgattctcg acaaaccggt cggcatgacg tcgacgaggg
     1261 ccgtgagcgc cgtgcgccgc atcttcgatg cccgcaaggc gggtcacggc ggcaccctcg
     1321 atccgcttgc caccggcctg ctgccgatcg cgctgggcga ggcgaccaag accgtgtcct
     1381 tcgtcatgga cggcgccaag acctaccggt tcaccgtccg ctggggcatc gggaccacga
     1441 ccgacgacac cgagggcgag ccgaccgcca catcggacgt caggccgtcg cgcgccgcca
     1501 tcgaggcggt cctgccgcgc ttcgccggta ccatatccca ggtcccgcca cgcttttccg
     1561 ccgtcaaggt ggccggcgag cgcgcctatg acctcgcccg cgacggcggg gagcccgagc
     1621 tcgatgcgcg cgaggtgcgg atcgaccgtc tcgacctggt cgagatggcg ggcgccgacg
     1681 agaccgtgtt cgaggccgac tgcggcaagg gcacctatgt gcgctcgctt gcccgcgacc
     1741 tgggccgggc gctcggcacc ttcgcccacg tgaccgccct gcgccggacc cgggtcgcgg
     1801 gcttcggcga ggacgacatg atttcgctgg aaaagctgga agagttgagc catattgccg
     1861 ccggccaaga gaccttggcc gacgtgctat gctccatgga gaccgcgctg gacgacatcc
     1921 cggcgttggc cgtaagcgga gcggacgcgg cgcgcctcat gcgaggccag gccgtaatct
     1981 tgcgcgggcg ggacgcaccc atccttcacg gaacggtact tgtcgcatcg ggcggtaatc
     2041 cggtggcgct tgccgaaatg aagcagggcg cgctccatcc caagcgcgta ttcaacctcg
     2101 ccggctgaac gccggccaac gacaacggag tgcacgatgt cgattaccgc ggagcgcaag
     2161 agcgctctca tcaaggaatt cgccaccaag gacggggaca ccggctcgcc tgaagtgcag
     2221 gtcgccatcc tgaccgagcg gatcaagaac ctcaccgatc atttcaaggg tcacgccaag
     2281 gacaaccatt cccgccgtgg cctgctcaag atggtcagcc agcgccgtag cctgctcgac
     2341 tacgtcaagt cgcgtaacga agagcgttac caggatctca tcaagcgact cggcatccgc
     2401 cgctagtcgc aaacgccggc catgccccct cgtcgggggc gacgccgatt cagaaggggt
     2461 gtccggcaat ggggccgggc gccattcatc gccccgtatc gaatgccgct gtggaagtgg
     2521 ttgaatcgca tcgaagagcg gccccggggc agacaggaag aagacatgtt caaaatccat
     2581 cgtgaagaaa tcatgtgggg cggacgcccg ctggtcctcg agaccggccg catcgcgcgc
     2641 caggccgacg gcgccgtgct ggtgacctac ggcgagacca ccgtgctcgc caccgcggtc
     2701 gccgaccgcc agcccaagcc cggcctcgat ttctttcccc tcaccgtcaa ctaccaggaa
     2761 aagacctatg ccgccggcaa gatcccgggc ggctacttca agcgcgaagg ccgtccgacc
     2821 gagaaggaga cgctgacctc gcgcctgatc gaccggccga tccggccgct gttcgccgac
     2881 ggcttccgca acgagaccca gatcatctgc accgtgctgt cgcacgatct ggagaacgac
     2941 cccgacatcc cggcgatggt cggcgcctcg gcggcactga ccatatcggg catcccgttc
     3001 ctggggccga tcggggccgc gcgcgtcgcc tatgtcgacg gcgtcctcgt ggccaacccg
     3061 acccacgccg agctccaggc gagcgagctc gacctggtcg tcgccggcac cgcgagcggc
     3121 gtgctgatgg tcgaatcgga agcgcgcgag ctcgccgagg aggtgatgct gggcgcggtg
     3181 atgttcggcc accgcgagat gcagccggtg gtcggggcga tcatcgagct ggccgagagg
     3241 tgcgccaagg agccgtggga cctgccgtcc aagcccgacc tcgcgccgac ccggcgccgg
     3301 gtcgaggaga tcgcgcgcgc cgagctcgag caggcctacg gcacggtggt caagcaggat
     3361 cgggtgcagc ggctcgatgc ggtccgcggc cgaatcgccg acgcgttcgc cgccgacgag
     3421 gccgaggccc ataccgccgg cgcggtgctc aagagcctgg agaaggacat cgtgcgcggc
     3481 gcggtgctgg ccaccggccg gcgcatcgac ggccgcgaca cgcgcaccgt gcgcccgatt
     3541 cgggccgagg tcggcgtgct gccgctggcc cacggctcgg cgctgttcac ccgcggcgag
     3601 acccaggcgc tggtggtcac cacgctcggc accggccagg acgagcagat cgtcgatgcg
     3661 ctcgaaggcg agtaccgcca gcacttcatg ctgcactata acttcccgcc ttactcggtc
     3721 ggcgaggcga gcttcctgcg cgggcccggc cggcgcgaga tcggccacgg caagctcgcc
     3781 tggcgggcgg tcaacccgct gatgccggcg aaggagagct ttccctacac cgtgcgcgtg
     3841 gtctccgaga tcaccgagtc caacggctcc tcgtcgatgg ccacggtgtg cggggcgtcg
     3901 ttgtcgctga tggacgccgg cgtgccgatg aaggccccga tcgcgggcat cgccatgggc
     3961 ctgatcaagg acggcgacaa gcacgtggtg ctgtccgaca tcctcggcga cgaggaccat
     4021 ctcggcgaca tggacttcaa ggtcgccggc agcgcggccg gggtgacctc gctgcagatg
     4081 gacatcaaga tcaccggcat caccgaggcg atcatgcgcg ccgccctcga ccaggcgcgc
     4141 gaggggcgcc tgcacatcct ggacgagatg gccaaggcgt tgtcgcgctc gcgcgaccag
     4201 gtcagccaga acgcgccccg catcaccacg ctgaccatcc cgcgcgacaa gatccgcgag
     4261 gtgatcggca ccggcggcaa ggtgattcgc gagatcaccg agaccaccgg caccaagatc
     4321 gacatcgagg acgacggcac gatcaaggtg gcggcgacca cctcggaggc gggcgaggcg
     4381 gcggtcaagt ggatccgcga catcgtcgcc gagcccgagg tgggcgtggt ctacgacggc
     4441 accgtggtca aggtggtcga cttcggcgcg ttcgtgaact tcatcggcaa ccgcgacggc
     4501 ctggtccaca tcagcgagct ggcgccgagg cgcgtgaaga cggtgggcga ggtggtcaag
     4561 gaaggcgaca aggtgaaggt taaggtgctg gcgatcgacg accgcggcaa ggtgcggctc
     4621 agcatgaagg tggtcgacca gcacaccggc gccgccgtca ccgacgaggc ggtgccgggc
     4681 tgagcccggc tgctgcccgg gtccgccgcc gggcccgggc ggtcagccgc cgttgccggc
     4741 gcccgcgcgc ggcaggtcga gggcatcgag aatgccgtcg accacgatct cgaccaccag
     4801 cgcgatgcgc cgcggcgtgt actcgctcca gccgagattg cgcagcacca gcggccggtt
     4861 gcgcaggaag atcaggcact cgccgagcag cgcattggtc aggatgacgg tctcctcctc
     4921 gcccggctcg cggccggtgg cggcgaccac caggtcggcg aaggcgtcgg tgaacggcgc
     4981 gaagatcgcc tgatacaggc gctcgaaggc gccggtcggg tggtcgatct cgcgcaggac
     5041 cagccgccac ggccactgct cgggcccgcg cccgaggagc gcggtgagca ccgcgcacac
     5101 gaacgccgat gcggcgcggg cgagcttcgc ccggtccgcg ccggcggccg cgagctcggc
     5161 gcgcaggcgc tgctcggcgc cgtcgatgag cttgcgcgtg tcggcgatgg tgtagtcgac
     5221 ggcctcgtta tagaggccgt ccttgccgcc gaaatagtag gcgatcgccg acaggttggc
     5281 gcccgcggtc ttggtcagct cccgggtcga gaccgcgtcc gggccgcgct cggcgaacat
     5341 catgccggcg gtgtgcagca gccgctcgcg ggcgctggtc gggtcggcgc acagcgcgtc
     5401 caggatctgg tcggtggttg gttccatgct tggcgcgcga cctcgagccg gccggcggcc
     5461 atcataggcc aaaccggttc ggtcgggaaa cgtcctggag gggtggcctt ttcagggcgc
     5521 tgggccatat aggggggcgt gttgaggcgc gcgaggagac gtcgcgcccg ccactgcgaa
     5581 ggttcgtgtt gaaggctctc agcccgatcg tgctgtccgg ccgggacgtg atgccgctgg
     5641 tcgaaggcgg caagggcatt tccgtgtcca acggccagag ctcgggcgcc tgggccgccg
     5701 ccggcgcggt gggcacgttc tccggggtca acgccgacgc gctcgacgag gccggcaacc
     5761 tgatcccggt ggtctatcac ggccgcaccc gccgcgagcg ccatgaggag ctgatcgcct
     5821 acgccatccg gggcggcatc acccaggcgc gcatcgccca cgagcagtcc tgcggcgagg
     5881 gccgcatcca catgaacgtg ctgtgggaga tgggcggctc ggagcgggtg ctcgagggcg
     5941 tgctgacggg cgcgcgcggg ctgatccacg gcgtcacctg cggcgccggc atgccctacc
     6001 ggatcgccga gatctcggcc cgccacggcg tctactacta cccgatcgta tcgtcggcgc
     6061 gggcgttccg ggcgctgtgg aagcgcgcct accacaagtt ccccgaatgg ctcggcggcg
     6121 tcgtctacga ggacccgtgg ctggccggcg gccacaacgg catcaccaac agcgaggacc
     6181 cgcagacgcc cgaggatccc tatccgcgcg tgctcgcgct gcgccgcatg atgtgcgagt
     6241 tcggcatcgg cgcgacgccg atcttcatgg ccggcggcgt gtggtacctg cgcgaatggg
     6301 acgactggat cgacaatccc gagctcggcc cgatcgcgtt ccagttcggc acccggccgc
     6361 tgctgaccca ggagagcccg atctcggacg cctggaagca gcggctgcgc gacctcaagc
     6421 cgggcgacat cagcctcaac cggttcagcc cgaccggctt ctggtcatcg gcggtgaaca
     6481 acgagttcct ggccgagctc cgcgcgcgcg cggcgcgcca ggtcgcctac accaccgaac
     6541 aggtcggcga gcaccgcatc ccgttcgaga tcgggccgcg caagcgaatc gcctatctga
     6601 ccgagcccga cgccgagcac gccgcgcaat ggtacgccga gggcttcacc gagggcttgc
     6661 gcacgcccga cagcacgctg gtgttcgtga cccccgagaa ggcggccgag atccgccgcg
     6721 accaggtcga gtgcatgggc tgcctgagcg cctgcaactt ctcgaactgg gcgcagaacg
     6781 accaccacac caccggccgg aagaccgatc cgcgcagcta ctgcatccag aagacgcttc
     6841 aggacatcgc ccatggcggc gacatccgca cccagctgat gttcgccggc cacggcgcct
     6901 atcgtttcgc caccgatccg ttctactcca acggcttcat cccgaccgtg cgcgagctga
     6961 tcgagcgcct gcagaccggc cactgagcgc gcgaaagcgg tttgtattcc accgctgcat
     7021 cgactatttt atatccagtc taaacaacgc cgggccccgg tcgaccatga cccgacccca
     7081 cgcggacccc gatctggcgt cgctgctgcg cgcagccggc ctgcggccga ccagccagcg
     7141 gcgcgcgctc gcgcgcctgc tgatcgaggg cgggccgcgc cacctcaccg ccgagctgct
     7201 gcacgccgag gcgcgcgcgc gcaagctcaa ggtgtcgctc gccaccgtct acaacacgct
     7261 gcaccagttc accgaggcgg gcctggtgcg cgagctggtg gtcgacgccg ggcgcgccta
     7321 cttcgacacc aataccagcc accaccacca cttctacgac gagagcgacg gccggctgat
     7381 cgacatcccg ggcgattcgg tggcggtgag cgcgttgccg gtgccgccgc ccggcaacga
     7441 gatcagccgg gtcgacgtgg tcgtgcgcct gcgccgcaag cgcgcgcagc cggccggctg
     7501 acgccgcccg cgaccaaacg tacaaatcgt tcttagaaca gttccaatct attgacaggc
     7561 tcgcggccgg ggcgcatata ggcgggtgcg cgcgtgcgcg cgccgtatcc gcaccaccca
     7621 ttttgcagga gggagatcat ggcgaaactg gccggcagca agaccgagca gaacctcaag
     7681 gacgccttcg ccggcgagtc gatggccaac cggcgctacc tgttcttcgc ccagaaggcg
     7741 gacgtcgagg gcttcaacga cgtctcggcg gtgttccgct cgaccgcgga aggcgagacc
     7801 gggcacgcct tcggccacct cgagtacctg atcgaggtcg gcgaccccgc caccggcaag
     7861 gcgatcgggg atacgtcgaa gaacctgaag gcggcgatcg cgggcgagac ccacgagtac
     7921 accgacatgt atccgggcat ggcgaagacc gcccgcaccg aaggcttcga ggagatcgcg
     7981 gactggttcg agaccctggc caaggccgag aggtcgcacg ccgggcggtt ccagaaggcc
     8041 ctcgccgcga tggactgagc gccgcggccg gcggcggacg gtgccggggc gcgccccgcc
     8101 ccgcccgccc cgcgcgcggc cacagcgccg ctgacgatcc tgcccccaag gtccgaggag
     8161 cttgccatgg aaggcggcct gaacgccccg gtccgccacc cgatccgcta ccgggacccg
     8221 gacttccacg atgccgacaa gctcgatgcc gagctcaggc gggtgttcga cgtctgccac
     8281 ggctgccggc gctgcttcaa cctgtgcgac tcgttcccgc gcctgttcga cctgatcgat
     8341 tcgtccgaga ccggcgagct cgactcggtc gacagcgcgg acttcgcccg cgtggtcggg
     8401 gcgtgcacgc tgtgcgacat gtgcttcatg accagctgcc cctacgtgcc gccgcacgag
     8461 ttcgacatcg acttcccgca tctgatgctg cgctatcgat cggcggagct ggccaagggc
     8521 aagctcggcg tcatcgaccg cgagctgacc aagaccgacc gcaacggccg cctcgccggc
     8581 gccgtggcgc cgctcgccaa ctggaccacc gatacccgca accggctcac ccgccccgcg
     8641 ctcgaggcgc tggccggcat ccaccgcgac gccgcgctgc cgcgcttcca tgcccgcagc
     8701 ttcgccaagc gcgccaagca ggcgccgccg gcggtgaacg ccgcggcacc ggcgcacggg
     8761 cgcaaggtgg cgctgttctc gacctgcttc gccaactaca acaaccccga catcgccatg
     8821 gcggcgctca aggtgctggc gcataacggc gtcgaggtcg aggtcgccta tcccggctgc
     8881 tgcggcatgc cgcagctcga gcacggcgac ttcgccgagg tggcccagcg cgcgacgcga
     8941 atcgcgccca tgctcggcgc ctggatcgac cgcggctacg acatcgtcgc gccggtgccg
     9001 agctgcgcgc tgatgctcaa gttcgagtgg ccgctggtgg tgcccggcga cgccgcggtc
     9061 aagcggctgg ccgagcatac cgccgacatc acccagtacg tggtcgacat cgcccgcacc
     9121 gagggcctgg ccgacggcct cgagccgctc gacggcggcg tcgcggtcca cctcgcctgc
     9181 catgcgcgcg cccagaacat gggcgccaag gcggtcgaga tgctcaggct gatccccgac
     9241 atcgagctcg aggtgatcga gcgctgctcg ggccacggcg gctcgctcgg gatcaagacg
     9301 gagttcttcg aggtcgcgct caaggtcggc cggccggcgg cgcgcaaggc gctgcaggcg
     9361 ggcaaggcct acctcgcctc ggaatgcccg ctggcagccg agcacctgcg ccagggcatc
     9421 gagcggctgg ccgaggacgc gccggtgccg gcgcatgcct ggcacccgat cgagctgctc
     9481 gcgcgcgcct acggcctcgc ggacgagtga ccgccatggc caggcacaag ctcgcccgcg
     9541 ccgacatcct gccgatggcc gagttcgcca aggtcaggcg cgaccgccgg cgcgcgctgg
     9601 ccgaggtcaa gcggcaccgc cgcgtcgccg tcgggccgca tgccacgttc tacttcgaga
     9661 accgcgacac catctggatg caggtgcacg agatgctgtt catcgagcgc ggcggcgagg
     9721 cgcagatcgc cggcgagctc gaggcctaca acccgctgat cccgaacggc cgcgagctgg
     9781 tcgcgacagt gatgttcgag atcccggacg tggccctgcg ccggcgcgtg ctcgccgccc
     9841 tgggcggcgt cgaggagacc atcgagctca ccgtcgccgg ccaggcgatc aagggcgagg
     9901 ccgagcgcga aatcgaccgc accaccgccg aaggcaaggc gtccgcggtc catttcgtcc
     9961 acttcccgtt cacccccgcc cagatcgccg cgtttcgcga ccccaaggc
//
LOCUS       DASWHU010000192        10794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3060981, whole genome shotgun sequence.
ACCESSION   DASWHU010000192 DASWHU010000000
VERSION     DASWHU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060981"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /gene="polA"
                     /locus_tag="VGB88_06975"
     CDS             <1..1799
                     /gene="polA"
                     /locus_tag="VGB88_06975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX9647224.1"
                     /translation="EAGRVAVDTETTSLDAMRAKLVGVSLSIEPGRACYVPLGHRPPD
                     GELIPGLDPGTEAPAQIPLDDALAVLRPLLEDPSVMKVGQNLKYDMLVLARPPYNIDI
                     APFDDTMLMSFVLDAGAHGHGMDELSTLHLGHTPIPIKDLIGTGKSQITFDLVPLQRA
                     CEYAAEDADVTLRLDHVLKPRLVAEHLVGVYQTLERPLAPVLVAMERRGIKVDKAALH
                     RASRDFAHRIAALEEEVFKLARHSFQIASTKQLGEVLFEEMGLAGGKKGKTGAYGTDA
                     GVLEDLAAQGHDLPARVLDWRQLTKLKSTYSDTLVEQINPDTGRVHTSYAMAGAATGR
                     LSSTDPNLQNIPVRTEEGRKIRRAFVADDGFRLISADYSQIELRLLAHVAGIEALRQA
                     FRDGADIHALTAAQVFGVAEKDMDPLMRRRAKTINFGIIYGISAFGLARQLGIGQGEA
                     RAYIDAYFDRYPGIRAYMDETKAFARANGHVRTIFGRRCHLSGINDRNPARRGFAERQ
                     AINAPLQGSAADIIKRAMIRMQPALDAANLSARMLLQVHDELVFEVPEGEVEETAAVV
                     KQVMEHAALPVLELSVPLTVDVGVGDNWDQAH"
     gene            complement(1938..3005)
                     /locus_tag="VGB88_06980"
     CDS             complement(1938..3005)
                     /locus_tag="VGB88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEX9647225.1"
                     /translation="MSAAAAFKVGHAAAGDWRAAAGRCLDQIGAGAGDTLGLVYATDA
                     LAGRLGDIVAWLRERTGIATWTGATGHGILAGDTDYIGAPALAVMCAALPADAIAPID
                     AALPPGMPFGIVHGDSRSPDLEHCIARASGGGFLVGGLVLPDGARVGAPAQAGAPLTG
                     LACAAAVPVATGLTQGCIPIGPAHEVTLCDAHLVAELDGRPALEVFLDEAGEILSRSR
                     AGGAGEVFAALPVAGSDTGDYLVRNIFGIDPGAGVVAVAAELTHGERMMFCRRDRASA
                     VEDLARMLARLKARLTAPARGALYVSCLARGPHLFGPGAVEAKAIQDAIGPLPLVGFY
                     ANGEICHDRVYTHTGVLTLFL"
     gene            3681..4664
                     /locus_tag="VGB88_06985"
     CDS             3681..4664
                     /locus_tag="VGB88_06985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX9647226.1"
                     /translation="MRRIAVPLIVMSVAAAAVPGARAVEVEDVLRALVRVEAEIPSDA
                     RTAAFLGTRREASGVVIDDAGLVLTIGYIILEAMSATVTDADGLEVPADVVAYDYDSG
                     FGLLRAIRPIAGAPLRLGDSDRLGPGDPALVVAAGGAAAILPVRITRRDPFAGWWEYL
                     LESAIYTAPAHPAWSGAALVGADGRMLGIGSLRLDDAGPGGAEESGNLFVPINLLKPI
                     LGELLAEGRRAAAPRPWMGLFTRDIASHVVVMRVIEDGPGARAGVQEGDVIAEVDGRA
                     VADMADLFRKVWALGEAGVEVPLTVARGPARQRIVVRSSDRYRYLKLDPSY"
     gene            4802..5842
                     /locus_tag="VGB88_06990"
     CDS             4802..5842
                     /locus_tag="VGB88_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9647227.1"
                     /translation="MAKSGAAAKRVSNDRQAPSARQLLSASAVTLHQLRIFWAVAQAR
                     SFTKASKLLDLAQPSLSQQISKLEEEVGAQLFSRSRNEMQVTDAGRFLLRKAELILSS
                     VDEALVGLAEFAAGSRGVIAVGALSSIARNVLPAAMRLLSERFPEVEIDIHETAPAEA
                     VDLLYGRRLNVALLATDSAAASGTSFHRTTVLSDPYVLAVPPGLDLARVQDVETDLGP
                     AERAVVNSTIQFNFGNQHKKRIEEWYQHVLPHYRVVAQSRTYEVALSLVQEGLGVAVV
                     PALTASLGAERAFDITLYRTTLPDRDVVALTPSQYARTEPYASFLRVLRESGRQIKLP
                     AIEPLPPLLKAL"
     gene            6019..6093
                     /gene="pqqA"
                     /locus_tag="VGB88_06995"
     CDS             6019..6093
                     /gene="pqqA"
                     /locus_tag="VGB88_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEX9647228.1"
                     /translation="MWTKPVVVEVAVGLEINCYACADL"
     gene            6283..7407
                     /locus_tag="VGB88_07000"
     CDS             6283..7407
                     /locus_tag="VGB88_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647229.1"
                     /translation="MKGLTAIVGAIALIVGVVVGKFLIPSGGEGMQVAETPAAEPSAP
                     APLAAAGGDKPVRFKMASSFPGELVQLGDQGRRLEQRIKALSDGNMEFKFYEPGALVP
                     ALEIFDAVSSGSIDAGWTASGYWAGKIPALQFFTAVPFGPYAGETVAWLFYGGGADLW
                     EELYKPHNILSMPCNLLAPEGSGWFRNEINSVEDLKGLKMRFFGLGAKVMEKLGVSTQ
                     LLAGGDIFPALELGTIDATEYSMPAIDLDLGFHQVAKHYYFPGWHQPTSVGELMINLD
                     KWNGMTTHQQEIVRSACKENMLNGLAQGEAIQFKALQELQSKGVILHRWSPEILDALR
                     TAWGEVVAQEAANDKDFARVWASLSEFRDGYKLWAEYGNM"
     gene            complement(7464..8567)
                     /locus_tag="VGB88_07005"
     CDS             complement(7464..8567)
                     /locus_tag="VGB88_07005"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX9647230.1"
                     /translation="MKKLRATLFSMVLAGAVLGGGAYGVEWWQLRQYVITTDNAYVRG
                     DITAISSRVDGYVEEVHVDGNQTVAAGEPLVTLRADRFETDVDEARADAGAAAAEVAI
                     GRAAVDNARARRRLQQSLIAQADARLDAAKAQAEQAAREVERYRQLLERKTGSRQKYE
                     AVATAQQTMEADVRRAQAELAAARDEVPVIDSEIHQLETEITRLEAKVARARSGLDRA
                     EIALSDTVIVAPVAGKIGNQRVERGMYMEAGWPMMSVVPLDGTYVVANFKETQLQRLR
                     IGQEAVMRVDAFPDHVLIGRIESLAPASAAEFSLLPAQNATGNFIKVVQRIPIKIVYD
                     LPPELDGRVLPGMSVVVTVDTRSAPEGAALAGQ"
     gene            8800..9636
                     /locus_tag="VGB88_07010"
     CDS             8800..9636
                     /locus_tag="VGB88_07010"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9647231.1"
                     /translation="MTAIDRSLTADALAARVPDGASIVMPTDANGVPMAVVRALIRRG
                     ARHLHLIGGPTSGLHADLLIGAGACDSIETAAVSLGEFGGAPRFRAAVESGRLRILDT
                     TCPALHAGLQAGEKGIPFMPLRGLIGSDLVGVRDDWRVIDNPFADGEDAVVLLPAIVP
                     DVALIHAACADSEGNVWIGLRREAMCMARAARRTLVTVEASRAGSLFDDPALAAGTLP
                     ALYVEAVAECARGAWPLAFADHYGADGAHLSAYAQAARSDDGFRAYLDRYVLAEPAAA
                     AE"
     gene            9652..10401
                     /locus_tag="VGB88_07015"
     CDS             9652..10401
                     /locus_tag="VGB88_07015"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9647232.1"
                     /translation="MAEAFAGRALLIAALARRLPAAGHVVVGAISPLPAAAALLAERR
                     ARGALRVTILGSARHYRFSDGGVELFDAAGRGRIDVFFLSGGQIDGQANINLVGTGTY
                     PNATTRFPGSFGSAYLYYVVPKVILFREEHSLRVLVPKVDFVSAPGTSPPGVWRPGGP
                     SALVTGKAAFAFDRARARFRLESVHPGETVESIIAATGFDFDRPARVATTPVATADEI
                     AAIQGPVRAALAPTYPEFAAALAPAGAAAEA"
     gene            10469..>10794
                     /locus_tag="VGB88_07020"
     CDS             10469..>10794
                     /locus_tag="VGB88_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647233.1"
                     /translation="MATKSQPTSAMGLGRMALLIGGGVAIIVGGTLLALNAGTRRVET
                     FGLSGLVEGGRGDADRPLTPLEQARRSFNDQYDVTPEPRNPEAAGTKLMLRAVAELNA
                     RERDIK"
BASE COUNT         1536 a         3819 c         3862 g         1577 t
ORIGIN      
        1 tcgaggccgg ccgggtcgcg gtcgacaccg agaccacctc gctcgatgcc atgcgcgcga
       61 agctcgtcgg cgtgtcgctg tcgatcgagc cggggcgcgc gtgctacgtg ccgctcggcc
      121 accgcccgcc cgacggcgag ctgatccccg ggcttgaccc ggggaccgag gcgccggcgc
      181 agatcccgct cgatgatgcg ctcgccgtgc tcaggccgct gctcgaggac ccctcggtga
      241 tgaaggttgg ccagaacctc aaatacgaca tgctggtgct ggcgcgcccg ccctacaaca
      301 tcgacatcgc cccgttcgac gacaccatgc tgatgtcgtt cgtgctcgac gcgggtgccc
      361 acggccacgg catggacgag ctcagcacgc tccatctcgg ccacacgccg atcccgatca
      421 aggacctgat cggcaccggc aagtcgcaga tcaccttcga cctggtgccc ctccagcgcg
      481 cctgcgagta cgcggccgag gacgccgacg tgaccttgcg cctcgaccac gtgctcaagc
      541 cgcggctggt cgccgagcac ctggtcggcg tctaccagac gctcgagcgc ccgctggcgc
      601 cggtgctggt ggcgatggag cgccgcggca tcaaggtcga caaggctgcg ctgcaccggg
      661 cctcgcgcga cttcgcgcac cgcatcgcgg cgctcgagga ggaggtgttc aagctcgcca
      721 ggcacagctt ccagatcgcc tcgaccaagc agctcggcga ggtgctgttc gaggagatgg
      781 gcctcgccgg cggcaagaag ggcaagaccg gcgcctacgg caccgacgcg ggcgtgctcg
      841 aggacctcgc cgcccagggc cacgatttgc ccgcgcgggt gctcgactgg cgccagctca
      901 ccaagctcaa gagcacctac tccgacaccc tggtcgagca gatcaacccc gacaccgggc
      961 gggtgcacac gtcttacgcc atggccggcg ccgccaccgg ccggctcagc tcgaccgacc
     1021 ccaacctcca gaacatcccg gtgcgcaccg aggaagggcg caagatccgg cgcgcgttcg
     1081 tcgccgatga cggcttccgg ctgatcagcg ccgattactc gcagatcgag ctgcgcctgc
     1141 tcgcccacgt cgccggcatc gaggcgctgc gccaagcgtt ccgcgacggc gccgacatcc
     1201 acgcgctgac cgcggcgcag gtgttcggcg tcgcggaaaa agacatggac ccgctgatgc
     1261 gccggcgcgc caagaccatc aatttcggca tcatctacgg catcagcgcg ttcggcctcg
     1321 cccgccagct cggcatcggc cagggcgagg cgcgtgccta tatcgacgcc tatttcgacc
     1381 gctatcccgg catccgcgcc tacatggacg agaccaaggc gttcgcccgc gccaacggcc
     1441 acgtgcgcac catcttcggg cgccgttgcc acctgtccgg gatcaacgac cgcaacccgg
     1501 cgcggcgcgg cttcgccgag cgccaggcga tcaacgcgcc gctgcagggc tcggccgccg
     1561 acatcatcaa gcgcgccatg atccgcatgc agccggcgct tgatgcggcg aacttgagcg
     1621 cgcgcatgct gctccaggtc cacgacgagc tggtgttcga ggtgccggaa ggcgaggtcg
     1681 aggagaccgc ggcggtggtc aagcaggtca tggagcacgc cgccctgccg gtgctcgagc
     1741 tcagcgtgcc gctgaccgtc gacgtcggcg tcggcgacaa ctgggaccag gcgcactgac
     1801 cgacggatag ccgtcatgcc ggccaagcgc cgggaaaccg gcgcgccgag ccggcatcca
     1861 tcgtgccaca cgtaccgccg aatggacccc ggctcttcgg ccggggtgac gccagggtca
     1921 agccgcgggg tcgcgcctca caggaacagg gtcagcacgc cggtgtgggt gtagacgcgg
     1981 tcgtggcaga tctcgccgtt ggcgtagaag ccgaccagcg gcagcggccc gatcgcgtcc
     2041 tggatggcct tggcctcgac cgcgccgggg ccgaacaaat gcgggccgcg ggcgaggcac
     2101 gagacgtaga gcgcgccccg cgccggcgcc gtgaggcgcg ccttgaggcg ggccagcatg
     2161 cgcgccaggt cctcgaccgc cgacgcgcgg tcgcggcggc agaacatcat ccgctcgccg
     2221 tgcgtgagct cggcggcgac ggcgaccacg ccggccccgg gatcgatgcc gaatatgttg
     2281 cgcactaggt agtcgccggt gtcggagccg gccaccggca gggcggcgaa cacctcgccc
     2341 gcgccgcccg cgcgcgaacg cgacaggatt tcgcccgcct cgtcgaggaa cacctcgagc
     2401 gcggggcggc cgtcgagctc ggcgaccagg tgcgcgtcgc acagggtcac ctcgtgcgcc
     2461 ggcccgatcg ggatgcagcc ctgggtgagc ccggtcgcga ccggcaccgc cgccgcgcag
     2521 gcgaggccgg tgagcggcgc gcccgcttgc gccggcgcgc cgacccgggc gccgtcgggc
     2581 agcaccaggc cgccgaccag gaagccgccg ccgctcgcgc gcgcgatgca gtgctcgagg
     2641 tcggggctgc ggctgtcgcc gtggacgatg ccgaacggca tgccgggcgg cagcgcggca
     2701 tcgatcggcg cgatcgcatc ggcgggcaag gccgcgcaca tcaccgccag cgccggcgcg
     2761 ccaatgtaat cggtgtcgcc ggcgaggatg ccgtggccgg tggcgccggt ccaggtggcg
     2821 atgccggtgc gctccctgag ccaggcgacg atgtcgccca ggcggccggc gagcgcgtcg
     2881 gtggcgtaga ccagcccgag cgtgtcgccg gcgccggcgc cgatctggtc gaggcagcgg
     2941 ccggcggccg cgcgccagtc gccggcggcg gcgtggccga ccttgaacgc cgcagcggcc
     3001 gacatgccgg tcgttccctc ctgccggtgc agtggcgact gcgccttagc accgccgtgc
     3061 gcgtgccgca agcgctcccg tcgggcgctc ccgcccccaa ctcatcgccg gcgatagcgg
     3121 gcgcacgcct gcaagcacct gacctatgtg ctagtattgg cggtggtcgc ccatggagaa
     3181 cccgaacatg cgagtttgct cggttggtag ttaatggtgt gaacatgagg gggttacttg
     3241 tagttctctc cgccagcctg ctcctggtcg gcgcggcgac ggcccaaacg gtagatgatt
     3301 tttcaatcga tcaaaggcgg gagctaactg tttttggaat ccgcggcggg ctcggcattg
     3361 tggtgggtcc tggcgacctc catgaagtac cctctggagg tgatttggag acaatgatag
     3421 cgaccggcct gaatgggaat ggtttcctat gtgcttcaat tagtgaaatc cgggcgctca
     3481 gcctccgggg caagtacgaa gtaacctgcg tggcttaccg tggcggctcc ggcacgaaga
     3541 cctacgtcat cgatgcgtta actggtgtcg cgtttgagca ataagtgttg aatgcgccaa
     3601 actactcagc ggtctgcgct ggcgagacgc gggcggctga tttaggctcg cagggcaatc
     3661 atgtcgatgg gtggtggcag atgcgccgca ttgcagtgcc gttgatcgtg atgtcggtgg
     3721 ccgcggcggc cgtgcccggg gcgcgcgcgg tcgaggtcga ggacgtgctg cgcgcgctgg
     3781 tgcgggtcga ggccgagatc ccctcggacg cgcgcaccgc cgcgttcctc ggcacccggc
     3841 gcgaggcgag cggggtggtg atcgacgacg ccggcctggt gctgaccatc ggctacatca
     3901 tcctcgaggc gatgtcggcg acggtgaccg acgccgacgg gctcgaggtg cccgcggacg
     3961 tggtcgccta cgactacgac tcgggcttcg ggctgttgcg cgccatccgc ccgatcgccg
     4021 gcgcgccgct caggctcggc gactcggacc gcctcggccc gggcgacccg gcgctggtgg
     4081 tcgccgccgg cggcgccgcc gcaattctgc cggtgcgcat cacccggcgc gatccgttcg
     4141 ccggctggtg ggagtacctg ctggagagcg ccatctacac cgccccggcg caccccgctt
     4201 ggtcgggcgc ggcgctggtc ggggcggacg ggcgcatgct cggcatcggc tcgcttcgtc
     4261 tggacgatgc cggccccggc ggcgccgagg aatccggcaa cctgttcgtg ccgatcaacc
     4321 tgttgaagcc gatcctgggc gagctgctcg ccgaggggcg ccgggcggcg gcgccgaggc
     4381 cgtggatggg gctgttcacc cgcgacatcg ccagccatgt ggtggtgatg cgggtgatcg
     4441 aggacggccc cggcgcgcgc gccggggtcc aggaaggcga cgtgatcgcc gaggtcgacg
     4501 gccgggcggt cgccgacatg gccgatttgt tccgcaaggt atgggcgctc ggcgaggccg
     4561 gggtcgaggt gccgctgacc gtggcgcgcg gcccggcgcg ccagcgcatc gtggtgcgct
     4621 cgtccgaccg ctaccgctac ctcaagctcg acccgagcta ctgagcggca gccgagcggg
     4681 cgaatagccg ctattggttg cacccgcgcc gccggctggc gtaccgtctg gcgcccggcg
     4741 gtgcggcctg cgtccgcggg cccgtgcaaa gacaacaaga cgacgaccac acagggggtc
     4801 catggccaag tccggtgccg ccgccaagcg ggtgtcgaac gaccgccaag cgccctccgc
     4861 caggcagctg ctcagcgcca gcgcggtcac gctgcaccag ctgcgcatct tctgggcggt
     4921 ggcccaggcg cggtcgttca ccaaggcgag caagctcctg gacctcgccc agccgtcgct
     4981 gtcgcagcag atctcgaagc tcgaggagga ggtcggcgcc cagctgttca gccgctcgcg
     5041 caacgagatg caggtgaccg acgccggccg cttcctgttg cgcaaggccg agctgatcct
     5101 gtccagcgtc gacgaggcgc tggtcgggct ggccgagttc gccgccggca gccgcggcgt
     5161 gatcgcggtc ggcgcgctga gctcgatcgc gcgcaacgtg ctgccggcgg cgatgcggct
     5221 gttgagcgag cggttccccg aggtcgagat cgacatccac gagaccgcgc ccgccgaggc
     5281 ggtcgacctc ttgtacgggc gccggctcaa cgtcgccctg ctggcgaccg attccgcggc
     5341 cgcgtcgggg acctcgttcc atcgcaccac ggtgctgtcg gacccgtacg tgctggcggt
     5401 gccgccgggc ctcgaccttg cccgggtgca ggacgtggag accgacctcg gcccggcaga
     5461 gcgcgcggtg gtcaacagca ccatccagtt caacttcggc aaccagcaca agaagcgcat
     5521 cgaggagtgg taccagcacg tcctgcccca ctaccgggtg gtcgcccagt cgcgcaccta
     5581 cgaggtggcg ctgtcgttgg tccaggaggg gctgggggtg gcggtggtgc cggcgctgac
     5641 cgcgagcctg ggggcggagc gcgcgttcga catcaccctc taccgcacca cgctccccga
     5701 ccgcgacgtg gtcgcgctga ccccgtcgca atatgcccgc accgagccct atgcgagctt
     5761 cctgagggtg ctgcgcgagt ccggccggca gatcaagctg ccggcgatcg agccgctgcc
     5821 gccgctgctg aaggcgctct agccgcccgg ccgcggcgcc ggcgccggcg gcgataagaa
     5881 aaatcaatag cggctataga gtgcgcccgc ggcggcggcg ggtagggtgt gggcgcgggt
     5941 tgtcgcgcgg cgagacgtgc gatccctcga gaaaagcggt catgtgaccg gtttgatcca
     6001 ggcctgggag gcgaactcat gtggacgaag ccagttgtgg ttgaagtggc cgttggtctc
     6061 gaaatcaact gctacgcctg cgccgacctc taacggacgc gcacatatag ccgtttttta
     6121 aggccgccgg ctccgcaagg agccggcggt tccgtttttg cgcccggcgc cgatccggcc
     6181 gcgatccggg ccgtggccgg ccggcaaacg ttggggcagc gataatcgca ctcgggggct
     6241 atactcgcgg cctggggagg gcaccgccaa gggaggtcac agatgaaagg actgacagca
     6301 atcgtcggcg cgatcgcact gatcgtcggg gtcgtggtcg gcaagttcct gattccgtcg
     6361 ggcggcgagg ggatgcaggt cgccgaaacg ccggcggccg agccgagcgc gccggcgccg
     6421 ctggccgccg cgggcggcga caagccggtt cgcttcaaga tggccagcag ctttccgggc
     6481 gagctggtcc agctcggcga ccagggccgc cggctcgagc agcgcatcaa ggcgctgtcc
     6541 gacggcaaca tggagttcaa gttctacgag cccggcgcgc tggtgccggc gctcgagatc
     6601 ttcgatgcgg tctcgtcggg ctcgatcgac gccggctgga ccgcgtcggg ctactgggcc
     6661 ggcaagatcc cggcgctgca gttcttcacc gcggtcccgt tcgggcctta tgccggcgag
     6721 acggtggcgt ggctgttcta tggcggcggc gcggacctgt gggaggagct ctacaagccg
     6781 cacaacatcc tctcgatgcc gtgcaacctg ctcgcgcccg agggctcggg ctggttccgc
     6841 aacgagatca actcggtcga ggacctgaag ggcctcaaga tgcgattctt cgggctgggg
     6901 gcgaaggtga tggagaagct cggcgtctcc acccagctgc tcgccggcgg cgacatcttc
     6961 ccggcgctcg agctcggcac gatcgatgcc accgagtact cgatgccggc gatcgacctc
     7021 gacctcggct ttcaccaggt ggccaagcac tactacttcc ccggctggca ccagccgacc
     7081 agcgtcggcg agctgatgat caacctcgac aagtggaacg gcatgaccac ccatcagcag
     7141 gagatcgtca ggtcggcgtg caaggagaac atgctgaacg gcctcgccca gggcgaggcg
     7201 atccagttca aggcgctcca ggagctgcag tcgaagggcg tcatcctgca ccgctggtcg
     7261 cccgagatcc tcgatgcgct gcgcactgcc tggggcgagg tggttgccca ggaagcggcc
     7321 aacgacaagg acttcgcccg cgtctgggcg tcgctgagcg agttccgcga cggctacaag
     7381 ctgtgggctg agtacggcaa catgtagccg cgcccgccgc cgcgccaccc cggccggccg
     7441 ccctgcggca gccggggtgg gcgctactgg ccggcgagcg ccgcgccctc gggcgcgctg
     7501 cgggtatcga ccgtgaccac caccgacatg ccgggcagca cgcgcccgtc gagctcgggc
     7561 ggcaggtcgt agacgatctt gatcgggatg cgctgcacca ccttgatgaa gttgccggtg
     7621 gcgttctgcg ccggcagcag gctgaactcg gccgcgctcg ccggcgccag gctttcgata
     7681 cgcccgatca gcacgtgatc ggggaaggcg tcgacgcgca tcaccgcttc ctggccgatc
     7741 ctgagccgct ggagctgggt ctccttgaag ttggcgacca cgtaggtgcc gtcgagcggc
     7801 accaccgaca tcatcggcca gccggcttcc atgtacatgc cgcgctcgac ccgctggttg
     7861 ccgatcttgc cggcgacggg ggcgacgatc accgtgtcgg agagcgcgat ctcggcccgg
     7921 tcgaggcctg agcgcgcccg cgccaccttg gcctcgagcc gggtgatctc ggtctcgagc
     7981 tggtggatct cgctgtcgat caccggcacc tcgtcgcgcg ccgcggcaag ctcggcctgg
     8041 gcgcggcgga cgtcggcctc catggtctgc tgcgcggtcg ccaccgcctc gtatttctgc
     8101 cggctgccgg tcttgcgctc gagcagctgg cggtagcgct cgacctcgcg cgccgcctgc
     8161 tcggcctgcg ccttggcggc gtccaggcgg gcgtcggcct gggcgatcag cgactgctgc
     8221 aggcgccggc gcgcgcgggc gttgtcgacc gcggcgcggc cgatcgcgac ctcggccgcg
     8281 gcggcgccgg cgtcggcgcg ggcctcgtcg acgtcggtct cgaaacggtc ggcgcggagc
     8341 gtcaccagcg gctcgcccgc ggccaccgtc tggttgccgt cgacgtgcac ttcctcgaca
     8401 tagccgtcga cccggcttga gatcgcggtg atgtcgccgc gcacataggc attgtcggtg
     8461 gtgatgacgt actggcgcag ctgccaccac tcgacgccgt aggcgccgcc gccgagaacg
     8521 gcgccggcga ggaccatgct gaacagggtg gcgcgaagtt tcttcatcgg ttcgtggctc
     8581 ggcctgctcc agccccctcg gggccgcggc gcctcgccca gtggtggtaa gtatggcccc
     8641 ggcgggagcg gatgtgaacg cccgcgcctg caacccagcc ctgcgctggc gccggatcac
     8701 ggccggttcc ggcgcggttt gcgtcgccgg cgcacagggc gtaggtttgc cgggcgcccg
     8761 cagcccctat gtcgctggtg aggcgaagga acgccggtga tgaccgcgat cgaccgatcc
     8821 ctgaccgccg acgcgctggc cgcgcgcgtg ccggacggcg ccagcatcgt catgcccacc
     8881 gacgccaatg gcgtgccgat ggccgtggtg cgcgcgctga tccgccgcgg cgcgcgccat
     8941 ctgcacctga tcggcggccc gaccagcggc ctgcacgccg acctgctgat cggcgccggg
     9001 gcctgcgaca gcatcgagac cgcggcggta tccttgggcg agttcggcgg ggcgccgcgc
     9061 ttccgcgccg ccgtcgaaag cggccggctg cggatcctcg ataccacctg cccggcgctg
     9121 cacgcgggcc tccaggcggg cgagaaaggc atcccgttca tgccgctgcg cggcctgatc
     9181 ggctccgacc tggtcggcgt gcgcgacgac tggcgggtga tcgacaaccc gttcgccgac
     9241 ggcgaggacg ccgtcgtgct gctgcccgcg atcgttcccg acgtcgccct gatccacgcc
     9301 gcctgcgccg attccgaggg caatgtctgg atcggcctca ggcgcgaggc gatgtgcatg
     9361 gcgcgggcgg cgcggcgcac cctggtcacg gtcgaggcgt cccgcgccgg cagcctgttc
     9421 gacgacccgg cgctcgccgc cggcaccctg cccgcgctct acgtcgaggc ggtggcggag
     9481 tgcgcgcggg gcgcatggcc gctcgccttc gccgaccatt acggcgccga cggcgcgcat
     9541 ctcagcgcct atgcccaggc ggcgcgcagc gacgacggct tccgcgccta tctcgaccgc
     9601 tacgtactcg ccgagccggc ggcagcggcc gagtagccgc cggcccgcac gatggccgag
     9661 gcgttcgccg gccgcgcgct gctgatcgcg gcgctcgcgc ggcggttgcc cgctgccggc
     9721 cacgtcgtgg tcggcgcgat ctcgcccttg ccggcggcgg cggcgctgct cgccgagcgg
     9781 cgcgcgcgcg gtgcgctcag ggtgaccatc ctcggcagcg cccgccacta tcggttcagc
     9841 gacggcgggg tcgagctgtt cgatgccgcc ggacgcgggc gcatcgacgt gttctttctc
     9901 agcggcggcc agatcgacgg ccaggccaac atcaacctgg tcggcaccgg cacctatccc
     9961 aacgccacca cccgctttcc cggctcgttc ggctcggcct acctctatta cgtggtgccg
    10021 aaggtgatcc tgttccgcga ggagcacagc ctgcgggtgc tggtgcccaa ggtcgacttc
    10081 gtcagcgccc ccggcaccag cccgcccggg gtgtggcggc cgggcgggcc gagcgcgctg
    10141 gtcaccggca aggcggcgtt cgccttcgat cgcgcccgcg cccgcttccg cctcgaatcc
    10201 gtccatcccg gcgagacggt ggagtcgatc atcgccgcca ccggcttcga cttcgaccgt
    10261 cccgcccggg tggcgaccac gccggtcgcg accgcggacg agatcgcggc catccagggc
    10321 ccggtgcgcg cggcgctcgc ccccacctac cccgagttcg ccgccgcgct ggcaccggcc
    10381 ggcgccgcgg ccgaggcctg atcggcgccg aaatcgccct atattgaggt ccgcggccgc
    10441 aatcttgcga cgcggaggca cccggaccat ggcgaccaag agccagccaa cctcggcgat
    10501 gggcctcggg cgcatggcgc tgctgatcgg cggcggcgtc gccatcatcg tcggcggcac
    10561 gctgctcgcg ctcaatgccg gcaccaggcg ggttgagacc ttcggcctct ccggcctggt
    10621 cgagggcggg cgcggggacg ccgaccggcc gctgaccccg ctcgagcagg cgcgccgcag
    10681 cttcaacgac cagtacgacg tcacgcccga gccgcgaaac ccggaggcgg cgggcaccaa
    10741 gctgatgctg cgcgcggtcg ccgagctcaa tgcgcgcgag cgcgacatca agat
//
LOCUS       DASWHU010000193         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3061997, whole genome shotgun sequence.
ACCESSION   DASWHU010000193 DASWHU010000000
VERSION     DASWHU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061997"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VGB88_07025"
     CDS             <1..1229
                     /locus_tag="VGB88_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HEX9647234.1"
                     /translation="ESLSSVRAKVYGHRLGSEQLAEIMTDIVAGRYADVHLSSFITAC
                     SANGMDHDEMVGLTRAMVAAGDRLDWAVSPVVDKHSVGGLPGNRTTPIVVAIVTACGL
                     TMPKTSSRAITSPAGTADVMETLAPVDLDLATMRRVVEREGGCIAWGGAVRLSPADDT
                     LIRVERALDLDSEGQLVASVLSKKAAAGATHLVLDMPVGATAKVRTAADADRLNRHLL
                     GVAQALGIEARVLRSDGRQPVGRGIGPALEARDVLAVLQTSLDAPADLRERALTLAAA
                     LLELGGAAAPAAGAGLAEATLADGRAWAKFQAICIAQGGLRAPPRAAYTQPVDAERSG
                     AVEAIDNRRLARAAKLAGAPDAKAAGAEVHAHLGQQVEQGEPLFTLHAETAGELAYAR
                     DYVDNHVPIFAIGDTR"
     gene            1226..2131
                     /locus_tag="VGB88_07030"
     CDS             1226..2131
                     /locus_tag="VGB88_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9647235.1"
                     /translation="MRPLVVPLPGNEDLARSLALGLDAEPCALEARYFPDGESYVRLD
                     GPVAGRQVILACTLDRPDAKYLRLAFTAAAARDLGAVEVGLVCPYLAYMRQDRRFRAG
                     EAVTSTTFAEALSSWIDWLVTVDPHLHRRTSLAEIFAVPAEVVHAAPALADWIGDSVA
                     SPVLIGPDSESAQWVSAVAALAGAPFVVLDKVRRGDRDVEVSVPQLERWRRHTPVLVD
                     DIISTARTMIETVGHLKAAEMPSPVCLGVHGVFANDAYDALRAAGGARIVTCNTIAHE
                     SNGIDLTEPLVEAIRQFLESGARRT"
     gene            2253..>2712
                     /locus_tag="VGB88_07035"
     CDS             2253..>2712
                     /locus_tag="VGB88_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9647236.1"
                     /translation="MAGISDVAELRALILRLGAWGPVAIIALMTVAILVSPIPSAPIA
                     VTAGAVYGHLWGTVYVIAGAEAGAVCAFGVARVLGYDVLHRWFGDRLTTGWLGSQNAL
                     TAIVLVSRLLPFISFDLISYAAGLSVIAFWRFALATLAGIVPASFLLAHFG"
BASE COUNT          357 a          975 c          964 g          416 t
ORIGIN      
        1 tcgaatcgct cagcagcgtg cgtgccaagg tctacggtca ccgactgggc agcgagcagc
       61 tggccgagat catgaccgac atcgtcgccg gccgctacgc ggacgtccat ctgtcgtcgt
      121 tcatcaccgc gtgctcggcc aacggcatgg accacgacga gatggtgggc ctgacgcgcg
      181 ccatggtcgc ggccggcgac cggctcgact gggcggtctc gccggtggtc gacaagcact
      241 cggtcggcgg cctgcccggc aaccgcacca ccccgatcgt ggtcgcgatc gtcaccgcct
      301 gcggcctgac catgcccaag acctcgtcgc gcgccatcac ctcgccggcg ggcacggccg
      361 atgtcatgga gacgctcgcc ccggtcgacc tcgaccttgc caccatgcgg cgcgtggtcg
      421 agcgcgaagg aggctgcatc gcctggggcg gagccgtgcg cctgagcccg gccgacgaca
      481 cgttgatccg ggtcgagcgc gcgctcgatc tcgacagcga aggccagctc gtcgcatcgg
      541 tgctgtcgaa gaaggcggcg gcgggcgcga cccaccttgt gctcgacatg ccggtcggcg
      601 cgaccgcgaa ggtgcgcacc gcagccgacg cggacaggct gaaccgccat ctgctgggag
      661 tggcgcaggc gctcgggatc gaggcccgcg tcctgcgctc cgacggccgc cagcctgtcg
      721 ggcgcggcat cggccccgcc ctcgaggcgc gcgacgtgct ggccgtgctt cagacctcgc
      781 ttgacgcccc cgccgacctc cgggagcgcg cgctcaccct tgcggcggcg ctgctcgagc
      841 ttggcggcgc ggcggccccg gcggcgggcg ccggtctcgc cgaggcgacg ctcgccgacg
      901 gccgcgcttg ggcaaagttc caggccatct gcattgccca gggcggcctg cgcgcgccgc
      961 cccgcgccgc gtacacgcag ccggtcgacg ccgagcggag cggcgcggtc gaggcgatcg
     1021 acaaccggcg tctggccaga gccgcgaagc tcgccggcgc gcccgacgcc aaggccgccg
     1081 gcgccgaggt gcacgcgcat ctcgggcagc aagtggagca aggcgagccg ttgttcaccc
     1141 tgcatgccga gaccgccggc gagctcgctt acgcgcgcga ctacgtcgac aaccatgtgc
     1201 cgatcttcgc catcggtgac acccgatgag gcccctggtc gtgccgctgc cgggcaacga
     1261 ggacctggcg cggtcgctcg ccctgggact cgacgccgag ccctgcgcgc tcgaggcccg
     1321 atacttcccc gacggcgagt cctatgtgcg cctcgacggg ccggtcgccg ggcggcaggt
     1381 gatcctggcc tgcacgctcg accgccccga cgccaagtac ctgcgcctgg ccttcaccgc
     1441 ggccgcggca agggacctcg gtgccgtgga ggtcgggctg gtctgtccgt acctcgccta
     1501 catgcgccag gaccggcgct tccgtgccgg cgaggccgtg acctcgacca ccttcgccga
     1561 ggcgctgtcg agctggatcg actggctggt gacggtcgat ccgcacctcc atcggcggac
     1621 gtcgctggcc gagatcttcg cggtgccggc cgaggtcgtc catgccgcgc ccgcgctggc
     1681 ggactggatc ggggactcgg tcgcatctcc ggtcctgatc ggcccggaca gcgaaagcgc
     1741 ccaatgggtg tcggcggtcg ccgcgctggc gggagcgccg ttcgtggtgc tcgacaaggt
     1801 ccgtcgtggc gaccgcgacg tcgaggtctc ggtgccccaa ctcgagcgtt ggcgcagaca
     1861 taccccggtg ctggtcgacg acatcatctc gaccgcgcgg accatgatcg agacggtcgg
     1921 ccacctcaag gcggcagaga tgccatcgcc cgtgtgtctc ggggtccatg gcgtattcgc
     1981 gaacgatgcc tatgacgcat tgcgggccgc cggaggcgcg cgcatcgtga cctgcaacac
     2041 gatcgctcac gagtccaatg gcatcgactt gaccgagcct ctcgtcgagg ccatccggca
     2101 attccttgag tccggcgcgc ggcgaacgta acacggggag ccgcgcggcc ctgccgcgga
     2161 ggccggcgag gcttcgcctc aagctgatcg gcctcgccgg tctggggttg ggactgattg
     2221 ggctttcgtg gtgggcggcc caatcgccgg cggtggccgg gatttccgac gttgccgagc
     2281 ttcgggcgtt gatcctgagg ttgggggcgt ggggaccggt cgcgatcatc gccctgatga
     2341 ccgtcgccat cctggtgagc ccgatcccga gcgccccgat cgcggtcacg gctggagccg
     2401 tctacggcca cctgtgggga accgtctatg tgatcgccgg cgccgaggcc ggcgcggtgt
     2461 gcgccttcgg ggtcgcgcgc gtgctcggct acgacgtcct gcaccgctgg ttcggcgacc
     2521 ggctgacgac cggctggctg ggctcgcaaa acgcgctgac cgcgatcgtg ctcgtgagcc
     2581 gcctgctgcc gttcatctcg ttcgacctga tcagctacgc cgccggcctg agcgtcatcg
     2641 cgttctggcg cttcgcgctg gcgacattgg cgggcatcgt accggcgagc ttcctgctcg
     2701 cccatttcgg cg
//
LOCUS       DASWHU010000194         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1196819, whole genome shotgun sequence.
ACCESSION   DASWHU010000194 DASWHU010000000
VERSION     DASWHU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196819"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VGB88_07040"
     CDS             complement(<1..1338)
                     /locus_tag="VGB88_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX9647237.1"
                     /translation="MLLAALAPARADAPATALAALPLVEPAKVGAGSLLLRERDAGSD
                     EAARAFRLGPTVGTDVSIEVGGMVARAAVRQQFFNPVQQWVEGVYVFPLPENAAVDHL
                     RLRVGERVIEGMIKEREEARRVYETARAEGRKAGLLESERPNIFTTSIANIGPGEQVE
                     VEIEYQQVLRYDQGAFSLRFPMVVGPRYIPGAIQTVSLGAGGWSVEATDRVPDAGRIS
                     APVLPPDAGKTNPVRLRVALTPGFPIGAIESPHHEVDIAAAEDGRTTVTLAAEVVPAD
                     RNFELVWRPEASAMPTAALLDETRDDGNYVLAMILPPTPVADGDGRPAPLAREVIFVL
                     DVSGSMGGASIRQAKAALDLALARLSPADSFNVIAFNHATRTLFRDARAATADAVARA
                     QRFVDGLDADGGTEMAPALARALTGAPPQGKLRQVVFLTDGAVGNETELFAIID"
     gene            complement(1553..1810)
                     /locus_tag="VGB88_07045"
     CDS             complement(1553..1810)
                     /locus_tag="VGB88_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9647238.1"
                     /translation="MAAAARGGFDLILMDIEMPGIDGLEATRRIRAHGGAAGRTPIIA
                     LTANAEPGDRARVRAAGMNDHVAKPIDLAALAQAIERARTP"
     gene            complement(1997..2434)
                     /locus_tag="VGB88_07050"
     CDS             complement(1997..2434)
                     /locus_tag="VGB88_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9647239.1"
                     /translation="MATTATKTRPRPVKTPDKSFARGAFAIAKSAKAKWTVGLRDHFE
                     YRDLGMTRATGGKLMAQVIRARRPCDEPGDEHYHILDFQLVYVLKGSARVRFEGLGER
                     RLEAGDCMYQRPGIRHRVVEYSDDFEVIEITVPADFETVSVAP"
     gene            complement(2498..3319)
                     /locus_tag="VGB88_07055"
     CDS             complement(2498..3319)
                     /locus_tag="VGB88_07055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647240.1"
                     /translation="MTARAAPTSRVALVTGAARGIGAAIAKGLAADGVRVAALDVEAR
                     ALEALPGEVVKVAADISEPDQCEAAVRRIAEACGALDVLINNAGLGMNMIRDDHFARP
                     VRIGDIAPAQWRRIFDVNCMGAFLMTRAAVPAMVARGWGRIVNVTTSLFTMMNEGFAP
                     YGPAKAALESASAIWAKELDGTGVTVNVLVPGGPTDTAMVPASSPFARRDLIPVAAMV
                     PPARWLASPASDGVTGRRFVAALWPADAPPAEAAEIAGAPAAWPQLAGNVVWPGR"
     gene            complement(3316..>3871)
                     /locus_tag="VGB88_07060"
     CDS             complement(3316..>3871)
                     /locus_tag="VGB88_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9647241.1"
                     /translation="LAKAGGAAGDGPVLIVGVGGVGMAALSFARALHETAPLVADIDP
                     AKREAALAAGAGAAFDPADKGAAKAVRAATGGGVSAVLDFVGADSSFAFANAALGRRS
                     TLVVVGLLGGRMTMPLAMFPLKGITVTGSYVGSLGQFRAMMALARDGRVAPIPIQTRP
                     LADASRTLDDLRAGRVLGRAVLVP"
BASE COUNT          502 a         1429 c         1440 g          500 t
ORIGIN      
        1 gtcgatgatg gcgaacagct cggtctcgtt gccgaccgcg ccgtcggtga ggaacaccac
       61 ctggcgcagc ttgccctgcg gtggcgcgcc ggtgagcgcg cgcgccagcg ccggcgccat
      121 ctcggtgccg ccgtcggcat cgaggccgtc gacgaaccgc tgcgcccgtg ccaccgcgtc
      181 cgccgtggcc gcacgcgcgt cgcggaacag ggtgcgagtg gcgtggttga aggcgatgac
      241 gttgaagctg tcggccgggc tgagccgcgc cagcgcaaga tcgagtgccg ccttggcctg
      301 gcggatcgag gcgccaccca tcgagcccga gacgtcgagc acgaagatta cctcgcgcgc
      361 gagcggcgcc ggccggccgt cgccgtcggc aacgggcgtc ggcggcagga tcatggccag
      421 cacatagttg ccgtcgtcac gtgtctcgtc gagcagcgcc gcggtcggca tcgccgatgc
      481 ttccggccgc cagaccagct cgaagttgcg gtcggcgggc accacctcgg cggcgagcgt
      541 gaccgtggtg cgcccgtcct cggcggcggc gatgtcgacc tcgtgatgcg gcgattcgat
      601 cgcgccgatc gggaagccgg gggtgagcgc gacccgcagg cgcaccgggt tggtcttgcc
      661 ggcgtcgggc ggcagcaccg gcgccgagat gcgcccggcg tcgggcaccc ggtcggtcgc
      721 ctcgaccgac cagccgccgg cgcccaggct cacggtctgg atcgcgcccg gaatgtagcg
      781 cggcccgacc accatcggga agcgcaggct gaacgccccc tggtcgtagc gcagcacctg
      841 ctggtactcg atctcgacct cgacctgctc gcccgggccg atgttggcga tcgaggtggt
      901 gaagatgttg ggccgctcgg attcgagcag gccggccttg cgcccctcgg cacgcgcggt
      961 ctcgtagacc cggcgggctt cctcgcgctc cttgatcatg ccttcgatca cgcgctcgcc
     1021 gacgcgcagg cgcagatgat cgacggccgc attctcgggc agcgggaaca cgtacacgcc
     1081 ctcgacccat tgttgcaccg ggttgaagaa ctgctggcgg acggcggcgc gcgcgaccat
     1141 gccgccgacc tcgatcgaga cgtcggtgcc gaccgtgggc ccgaggcgga aggcccgcgc
     1201 ggcctcgtcg ctgccggcgt cgcgctcgcg cagcagcagg ctgccggcgc cgaccttggc
     1261 cggctcgacc agcggcaacg cggccagtgc ggtcgccggc gcatccgcgc gcgccggcgc
     1321 cagcgcggcc agcaacacaa caacggccgc gaccggaagc cggcagcggc cacccatccg
     1381 agtgagcgca tggtgaggca tggtttcccc cttgcacggg caccgggtcg ccgcgacccg
     1441 gcatcgcggt cacgttgcgc aggaaaccgg acggaattcg ggcgcggccg gtggcgggcg
     1501 aggggaattg tcggggcgat tttgtggcgg cggggctcgc cgccgccggg cgttacgggg
     1561 tgcgcgcgcg ctcgatcgcc tgggccagcg cggcaaggtc gatcggcttg gcgacgtggt
     1621 cgttcatgcc ggcggcgcga acccgggcgc ggtcgccggg ctccgcgttc gcggtcagcg
     1681 cgatgatggg ggtgcgcccg gcggcgccgc cgtgggcgcg gatgcgccgc gtcgcctcga
     1741 ggccgtcgat gccgggcatc tcgatgtcca tcaggatcag gtcgaagccg ccgcgcgccg
     1801 ccgccgccac cgccgcgcgc cgccgccgcc accgccgcgg cgccgtcggc gaccgcctcg
     1861 acgcggtggc cgaggtcgcc gagcaccgcg gcgaccagcc gccggttcac caggttgtcc
     1921 tcggcgagca ggatcgcgag cggccgccga tcggttacga cacccgcgcc cggcgacggc
     1981 cgcgccgccg cctcggtcac ggcgccaccg agactgtctc gaagtcggcg ggcacggtga
     2041 tctcgatcac ctcgaagtcg tccgagtact cgaccacgcg gtggcggatg ccggggcgct
     2101 ggtacatgca gtcgccggcc tccagccggc gctcgccgag gccctcgaag cgcacccgcg
     2161 ccgagccctt gagcacgtag acgagctgga agtcgaggat gtggtagtgc tcgtcgccgg
     2221 gctcatcgca cggcctgcgg gcgcggatca cctgggccat cagcttgccg ccggtggccc
     2281 tggtcatgcc gaggtcgcga tactcgaaat ggtcgcgcag ccccaccgtc cacttggcct
     2341 tggccgactt ggcgatggcg aacgcgccgc gcgcgaagct cttgtccggg gtcttgaccg
     2401 gcctgggcct ggtcttggtc gcggtggtgg ccatgggtcc tcccagcggt tcgactgtga
     2461 atgattgcgc cgtgccgggg cgagaatagc accaccctca gcggcccggc cagaccacgt
     2521 tgccggcaag ctgcggccag gccgcgggcg cgccggcaat ctcggccgcc tcggccggcg
     2581 gcgcatcggc cggccacagc gcggcgacga accggcgccc ggtgacgccg tcggaggcgg
     2641 gcgaggcgag ccagcgcgcg ggcggcacca tcgccgcgac cgggatcagg tccctgcgcg
     2701 cgaacgggct cgatgccggc accatggccg tgtcggtcgg cccgcccggc accagcacgt
     2761 tgaccgtgac cccggtgccg tcgagctcct tggcccagat cgccgacgcc gactcgagcg
     2821 ccgccttggc cgggccgtag ggcgcgaagc cctcgttcat catggtgaac aggctggtgg
     2881 tgacgttgac gatccgcccc cagccgcgcg cgaccatcgc cggcaccgcg gcgcgggtca
     2941 tcaggaacgc gcccatgcag ttgacgtcga agattcgccg ccactgcgcg ggcgcgatgt
     3001 cgccgatgcg caccggccgg gcgaagtggt cgtcgcggat catgttcatg ccgaggccgg
     3061 cgttgttgat cagcacatcg agcgcgccgc aggcctcggc gatgcggcgt accgcggctt
     3121 cgcactggtc gggctcggag atgtcggcgg cgaccttgac cacctcgccg ggcagcgcct
     3181 cgagcgcgcg cgcctccacg tcgagcgccg ccacgcgcac gccgtcggcg gcaaggccct
     3241 tggcgatcgc cgcgccgatg ccgcgcgcgg caccggtgac cagcgcgacc cggctggtcg
     3301 gtgcggcgcg cgcggtcacg gcaccagcac cgcgcgcccc agcacgcgcc ccgcgcgcag
     3361 gtcgtcgagg gtgcgcgagg catcggcgag cgggcgggtc tggatcggga tcggcgctac
     3421 ccgcccgtcg cgcgccagcg ccatcatggc gcggaactgg ccgagcgagc cgacatagga
     3481 gccggtcacg gtgatgcctt tcagcgggaa catcgcgagc ggcatggtca tgcgcccgcc
     3541 gagcagcccg accacgacca gcgtgctccg gcgcccgagc gcggcgttgg cgaacgcgaa
     3601 cgagctgtcc gcgccgacga agtcgagcac cgcgctgacg cctccgccgg tcgcggcgcg
     3661 caccgccttg gcggcgccct tgtccgccgg atcgaacgcc gccccggcac cggcggcgag
     3721 cgccgcctcg cgcttcgccg ggtcgatgtc ggcgaccagg ggcgcggtct cgtgcagcgc
     3781 gcgcgcgaac gacagcgccg ccatgccgac gccgcccacg ccgacgatca gcaccggccc
     3841 gtcgccggcc gctccgccgg ccttggccag t
//
LOCUS       DASWHU010000195         4527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1201215, whole genome shotgun sequence.
ACCESSION   DASWHU010000195 DASWHU010000000
VERSION     DASWHU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201215"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VGB88_07065"
     CDS             <1..661
                     /locus_tag="VGB88_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647242.1"
                     /translation="HECAHRVGMSLGAPESVDDIYQHTTRFLFLDTEEALLNSDSYAL
                     FAGAISGGVPVSFVIVEPSIGAGVAIPGRGAATWQARLYIGAEAQHPVLGIFNPTLGF
                     GMSLIGETTTGGPSPVAAGPSLLASLLAGVRIGDPRPGAAGGGYASFFGGPALAVGAS
                     ELKAGAEAGLALGYRWRWLDVSAGVGYAYDPTREAGMEHLFTLGASVSFLPFHVTAGG
                     R"
     gene            658..3585
                     /locus_tag="VGB88_07070"
     CDS             658..3585
                     /locus_tag="VGB88_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX9647243.1"
                     /translation="MTKTLAVASHGSQPVRRAAWAQSCRCAQRPAGASNRALQAKLRV
                     GPANDPLERAADRAADRVLAGGPAGALGRAGLGAQRACAACAAEQRETLRRQPVEGEE
                     QDEEQVQAKAEVGGPHAASAEAAAAAVASGGRQLSPGLRAYFEPRFGRDLADVRVHDH
                     ARAAGAARAIGARAYTLGRDVAFAPGAWAPASDDGRRLIAHELAHVVQQGSGRAVVVR
                     RQGDPDLPPPPDFAPPERRLPPMIEIVETPDRAEPGCPRPPTNLGNLTPVPPCAEDGE
                     DIDGKVFYFCPDSDVFLDAADLENFRSFVQGKLSGTQFRVRGYASVEGPGTAVQADAY
                     NRRLTCHRTKRMVRELINAGVQETQIEAVSLGPTERFGAGRDKRPLNRVAVVEAVPPT
                     RRTTPPDGGGTLTPAQVRDRARQLLVAGNYLLGADAYLARWSCGRFHNLSDAVSRTSV
                     QMEGVDFGGSVTELGTIGRFGANTIKLSSSVTNATDPTTCAANRIADLTFHHFARPQL
                     PAFGDQHRAGLHLVHLAGLPACTMRRDPLGLDNDDISVPLADDPFLGFQPACADMPLP
                     GPIGGRGHPSRAETPPTFTAGPIRLDGGSGSISGALDPAPSRPGAPTVGVEMSRPMTL
                     TGKVTASGDAATIATYEVGFVQTAMAEDWLNTYVDGRRERRRLPLPLRDGPGRDDPLS
                     DPPWFARFARVQAQPGDNDVTMTDAPNFRAFRVLPDLSTTRFVRSFDLPPATGGTARR
                     HDFAVFEPQATHPNNVPDWGHREIEFNTWLVARQANPRAPATLDSTVAIAGVRMTLRH
                     EVNWVVDSSGRIEGNGTFTVSSQPAAESDAAMLVLRGATAQDFVSPQGVPLFNEFLET
                     QGPLPRVSAGGLARNAYFAAVRKIVAPHRTTPRLRERFVVRIKVDMATGRVILDTPAL
                     EGRAIEIVPSETEGGPINPGEARTLARAIFPEVRKLVLRSLTSTDPESGVVTVPVVIT
                     AEP"
     gene            3667..>4527
                     /locus_tag="VGB88_07075"
     CDS             3667..>4527
                     /locus_tag="VGB88_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647244.1"
                     /translation="MSLGAAGATLAAPGASNQSALQRFAPQLPRLSAADLRAILAESE
                     RPVRAWLDANESLVATLSLAAATAEVLRRVPGAGDQGAGMIAGIVRDWAAERGLTLRP
                     VSLVPHPADAAAGPGARPSIATGDSGLIAAVRGAIGAVTEGIRIERPYGFTRIGVSGA
                     TVGLRPGESEISGTVTPSGGVRVRAGERGGRGEVEVSESGVAIAARPPSPGVRFKAGV
                     SWSGALNFSSSVANFHFAMSVTPERWSMTFTFPSGDMPADLSMLGRVFGDGESALRAA
                     LIETAGVRNLG"
BASE COUNT          594 a         1670 c         1647 g          616 t
ORIGIN      
        1 tcacgaatgc gcgcaccgcg tcggcatgtc gctcggcgcg ccggagagcg tcgacgacat
       61 ctaccagcac acgacacggt tcctgttcct cgataccgag gaagcgctgc tcaacagcga
      121 ttcctacgcg ctgttcgcgg gcgcgatcag cggcggcgtg ccggtctcgt tcgtgatcgt
      181 cgagccgtcc atcggcgccg gcgtcgccat cccggggcgc ggagcggcga cgtggcaggc
      241 gcggctctat atcggcgccg aggcccagca cccggtgctc ggcatcttca acccgacgct
      301 tggcttcggc atgtcgctga tcggcgagac gaccaccggc ggcccgtcgc cagtggcagc
      361 ggggccgagc ctgctcgcca gcctgctggc gggggtgcgg atcggcgacc cgcggcccgg
      421 cgccgccggc ggcggctatg cgtcgttctt cggcgggccg gcgctcgccg tcggtgcctc
      481 cgagttgaag gccggcgccg aggcgggact cgcgctcggc tatcgctggc ggtggctcga
      541 tgtctccgcc ggtgtcggct atgcctacga cccgacgcgc gaggccggca tggagcactt
      601 gttcacgctc ggcgcgagcg tgagcttcct gccgttccac gtcacggcag gcggcagatg
      661 accaagacct tggccgttgc gagccacggg tcgcagcccg tgcggcgggc ggcgtgggcg
      721 cagtcgtgcc ggtgcgcaca gcggccggcc ggtgcgagca accgcgcgct ccaggcaaag
      781 ctcagggtgg ggccggccaa cgacccgctc gaacgcgcgg ccgaccgagc ggcggatcgg
      841 gtgctggcgg gcggccccgc cggggcgctc ggccgcgcgg gcctgggcgc gcagcgcgcc
      901 tgcgcggcgt gcgcggcaga gcagcgggag accttgcgcc gccagccggt cgagggcgag
      961 gaacaggacg aggagcaggt ccaggccaag gctgaagtgg gcgggccgca cgccgccagc
     1021 gccgaggccg ccgccgcggc ggtggcgagc ggcggccggc agttgtcgcc cggcctgcgc
     1081 gcctatttcg agccgcgctt cgggcgagac ttggcggacg tgcgcgtcca cgaccatgcg
     1141 cgggcggccg gcgcggcgcg cgcaatcggc gcccgcgcct acaccctcgg ccgcgacgtg
     1201 gccttcgcgc ccggcgcctg ggcgcccgcc agcgacgacg gacggcgcct gatcgcccat
     1261 gagctcgccc acgtcgtgca gcaaggctcg ggccgcgcgg tggtcgtccg gcgtcagggc
     1321 gatccggacc tgccgccgcc accggacttc gcgccgccgg aacggcgttt gccgccgatg
     1381 atcgagatcg tcgagacccc cgaccgggcc gagccgggct gcccgcggcc gccgaccaat
     1441 ctcggcaacc tgacgccggt gccgccgtgc gccgaggacg gcgaggatat cgacggcaag
     1501 gtgttctatt tctgcccgga ttcggacgtg ttcctggatg ccgccgacct cgagaatttt
     1561 cgcagctttg tgcaaggcaa gctgtcgggg acgcagttcc gtgttcgcgg ctatgccagc
     1621 gtcgagggtc cgggcacggc ggtgcaggcc gacgcctaca accgccggct cacctgccat
     1681 cgcaccaagc gcatggtccg cgagttgatc aacgcgggcg ttcaggagac ccagatcgag
     1741 gcggtgtcgc tcggcccgac cgagcgcttc ggcgcgggcc gcgacaagcg tccgctcaac
     1801 cgggtcgcgg tggtcgaggc cgttccgccc acgcgccgga ccacgccgcc cgacggcggc
     1861 ggcacgctga cgccggccca ggtccgcgac cgcgcccggc agctgcttgt ggcgggcaac
     1921 tatctgctgg gtgcggacgc ctatcttgcg cgctggtcgt gcgggcgctt ccacaacctc
     1981 tcggacgcgg taagccgcac cagcgtacag atggaaggag tcgacttcgg cggcagcgtc
     2041 accgagctcg gcacgatcgg acggttcggg gcgaacacga ttaagctctc ttccagcgtc
     2101 acgaacgcca ccgatccgac cacctgcgcc gccaaccgca tcgccgacct gaccttccat
     2161 cattttgccc ggccgcagct tccggccttc ggcgaccagc atcgggccgg gcttcacctg
     2221 gtgcatctcg ccgggctgcc cgcctgcacc atgcggcgcg acccgctcgg cctcgacaac
     2281 gacgacatct cggttcccct ggccgacgac ccgttcctcg gcttccagcc ggcttgcgcc
     2341 gacatgccgc tgcccggccc gatcggcggc cgcggccatc cgagccgcgc cgagaccccg
     2401 cccaccttca ccgccggccc gatccggctc gacggcggct cgggcagcat ctccggcgcg
     2461 ctcgacccgg cgccgtcgcg gccgggcgcg ccgaccgtcg gcgtcgagat gtcgcggccg
     2521 atgacgctga cgggaaaggt caccgcctcg ggtgatgcgg cgacgatcgc cacctacgaa
     2581 gtcggcttcg tgcagacggc gatggccgag gactggctca acacctatgt cgacgggcgg
     2641 cgcgagcgcc gccggctgcc gctgccgctg cgcgacgggc cggggcgcga cgatccgctg
     2701 tcggacccgc cctggttcgc gcgcttcgcg cgcgtccagg cccagccggg cgacaacgac
     2761 gtgaccatga ccgacgcgcc caacttccgc gcgttccggg tccttcccga cctttccacg
     2821 actcgcttcg tgcgcagctt cgacctgccc ccggccaccg gcggcaccgc ccggcggcac
     2881 gacttcgccg tgttcgagcc gcaggcgacg catcccaaca acgttcccga ctggggccac
     2941 cgcgagatcg agttcaacac ctggctggtc gcccgacagg ccaatccgcg cgcgccggcg
     3001 accctcgatt cgaccgtcgc catcgccggc gtgcgcatga cgctccgcca cgaggtgaac
     3061 tgggtggtcg acagcagcgg gcgaatcgag gggaatggca ccttcaccgt ctcgtcgcag
     3121 cccgcggccg agagcgacgc ggcgatgctg gtgctgcgcg gcgccacggc ccaggacttc
     3181 gtcagcccgc aaggcgtgcc gctgttcaac gagttcctcg agacccaggg tccgctgccg
     3241 cgcgtgagcg ccggcggcct ggcgcgcaac gcctacttcg ccgcggtgcg caagatcgtg
     3301 gcgccgcacc gcaccacccc gcggctgcgc gaacggttcg tggtccggat caaggtcgac
     3361 atggcgaccg gacgggtgat cctcgacacg ccggccctgg aagggcgggc aatcgagatc
     3421 gtgccgagcg aaacagaggg cgggccgatc aatccgggcg aggcgcgcac tctcgcgcgc
     3481 gcgatcttcc ccgaggtccg caagctggtg ctgcgcagcc tgacctcgac cgatcccgaa
     3541 tccggggtcg tgaccgtgcc ggtcgtgatc acggccgagc cctgagccgg agccgagacg
     3601 cgcattatgg ccccgccggt ccgccccagc cgatcccgcc gagagccgtc acctgcgcgg
     3661 gcgccggtgt cgctcggcgc cgccggtgcc accctggccg cgcccggagc cagcaatcag
     3721 tcggcgctcc agcgtttcgc gccgcaactg ccgaggctct cggcggccga cctgcgggcg
     3781 atcctcgccg aaagcgaacg gccggtgcgc gcctggctcg acgccaacga gtcgttggtg
     3841 gcgaccctgt cgctcgccgc ggccaccgcg gaagtgttgc gtcgcgtgcc cggcgccggc
     3901 gaccaggggg cgggcatgat cgccggcatc gtccgcgact gggcggccga gcgcggcctg
     3961 accttgcggc cggtgtcgct ggtgccgcat ccggcggacg cggcggccgg gccgggtgcg
     4021 cggccgagca tcgcgaccgg cgacagcggc ctgattgccg cggtccgcgg cgcgatcggc
     4081 gcggtcaccg agggcatccg catcgagcgc ccgtacggct tcacacgtat cggcgtgagc
     4141 ggcgcgactg tgggcttgcg cccgggcgag agcgagatct cgggcacggt tacgccgagc
     4201 ggcggggtcc gcgtgcgcgc cggcgagcgg ggcgggcgag gggaggtcga ggtgagcgaa
     4261 agcggcgtcg ccatcgccgc gcgcccgcca agtcccggcg tccggttcaa ggccggggtg
     4321 agctggtcgg gcgcgctcaa cttctcgtcg agcgtcgcga atttccattt cgccatgtcg
     4381 gtcacgcccg agcgctggtc gatgacattc acgtttccgt cgggcgacat gcccgcggac
     4441 ctgtcgatgc tcggacgcgt gttcggcgac ggcgagtcgg cgctgcgcgc ggcgttgatc
     4501 gagaccgcgg gcgtgcgcaa ccttggc
//
LOCUS       DASWHU010000196         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_96899, whole genome shotgun sequence.
ACCESSION   DASWHU010000196 DASWHU010000000
VERSION     DASWHU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96899"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VGB88_07080"
     CDS             <1..1353
                     /locus_tag="VGB88_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9647245.1"
                     /translation="AGVYTTPAVYCEVKAVFTNTVPVDAYRGAGRPEACYLIERIVDV
                     AARECGLDAAEIRRRNFIPESAQPYQTPVAMVYDGGNYAKNMADCLKAADAAGFAARR
                     KDAKARGKLRGFGLACYIEACGAAPSAIAGALGARAGLYEMATVRVNPTGSVTVITGT
                     HSHGQSHETTFAQLVSERLSLPLEAIEVVHGDTDRIPFGMGTYGSRSLAVGGSALVKS
                     LDKVVDKGKRIAGHILEAAADDIEFANGRFTVSGTDRSLAFAEIAGAAYVPHNYPLAE
                     LEPGLEETSYFDPPNFTFPSGCYVCEVEVDPDTGTVSIERFTAVDDFGVVINPNVVEG
                     QVHGGLVQGIGQALYEGCRYDPENGQLITGSYMDYCMPKASDVPPFAVSTNVTLSPTN
                     PMGVRGCGEAGAIGAPPAVINAVVDALSDYGVTHIDMPATPQRVWRTIQGHAHARAAE
                     "
     gene            1370..2164
                     /locus_tag="VGB88_07085"
     CDS             1370..2164
                     /locus_tag="VGB88_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9647246.1"
                     /translation="MYDFTYHRAASVDQAKKLLAAAADGRYLAGGMTLLPTMKQRLAN
                     PSDLIDLAGIAELAGIRRDGKALVVGAMTTHAAVAASAEARKAIPALAALAGMIGDPQ
                     VRNRGTIGGSLANSDPAADYPAAVLALGATVRTDRREIAGDDFFTGMFETALDNGELI
                     TAVSFPVPEAACYMKFANPASRYAIVGVMVARTGAGVRVAVTGAGPCAFRVADMEKAL
                     AKKFAPEAIAGIGVPADDLNADIHAGADYRAHLIGVMAKRAVAGCA"
     gene            2178..3116
                     /locus_tag="VGB88_07090"
     CDS             2178..3116
                     /locus_tag="VGB88_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9647247.1"
                     /translation="MTALPASVDEALALLAAGHYVADRSLATALYLSLSMGRPLFLEG
                     EAGVGKTEIAKVLAERLDRRLVRLQCYEGLDIASAVYEWNYAAQMVEIQAAKAEGIAD
                     RARLIDDVFSRRFLIERPILQALEPDSRGAPVLLIDELDRADEPFEAYLLEVLADFQV
                     TVPEIGTIRAPSAPIVVITSNRTREIHDAIKRRCFYFWIDYPDARREKQILAARVPGA
                     PERLSGEAVAFVQRLRALDLFKKPGVAETIDWANALMRLDAIELDAETVDATLGVLLK
                     YQDDIARLRGTEAAAILDEIRRGSEGARARAAGAGA"
     gene            3113..4201
                     /locus_tag="VGB88_07095"
     CDS             3113..4201
                     /locus_tag="VGB88_07095"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX9647248.1"
                     /translation="MSGLRLIDLEGDAEARGQAHGRALKSAIADNVETYLARFESGGI
                     ARADALAEGARWGERIARFDEDYAAEMRAIAEAAGIDAAAVAMLNARWELSYGITARD
                     ALARDGCTAFAALPEITRTGTTLIGQNWDWLERLRGRLAVLRVRGRGHPDFLCITQAG
                     IAGGMIGLNAAGIGLCVNGMTSDRDGRDPERKPFHLRVREALRASSLATALKAFLGSP
                     RACSVNVLFGHAGGEAIDVEAAPDTESVLYPEGGLITHANHFERPPGVVSTLQWLRPG
                     TLYRARRLRRLFRQANRAVDVELAQELFADHFSRPHSICAHPDADVDEIERTTTVTSA
                     ILDLGNRVLYATDGPPCENPYRAYPLDR"
     gene            4198..>5336
                     /locus_tag="VGB88_07100"
     CDS             4198..>5336
                     /locus_tag="VGB88_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9647249.1"
                     /translation="MTAPARGPGHLADNVMHFCRTLRAAGLPVGPGKVIDALGAMART
                     GLARRDDVYWALHASLVNRRDQHELFDQAFHAFWRAPDLLERLAGLALPEFVRVAKAP
                     PAARRVAEALRAVRGEGEERAPETRELDAALTASGAEVLQKIDFERMTADEIALAKRA
                     IRELALSIAALPTRRFAPATHGARIHMRRTLRAALRGQGEAIPLVRRRRLRRPPPIVV
                     LCDISGSMGRYSRMLLHFLHALANDRDRVHVFAFGTRLTNVTRRLRQRDVDVAMDAVV
                     DAVEDWSGGTRIGDCLHDFNRAWSRRVLGQGAIVLLISDGLDRAAGVDLGREMERLHK
                     SCRRLIWLNPLLRYDGFEPKALGVRAMLPHVDEFRPVHNLETIAAL"
BASE COUNT          751 a         1993 c         1874 g          718 t
ORIGIN      
        1 gccggcgtct acaccacgcc cgcggtctac tgcgaggtca aggcggtgtt caccaacacc
       61 gtgccggtcg acgcctatcg cggcgccggg cggcccgagg cgtgctacct gatcgagcgc
      121 atcgtcgacg tggcggcgcg cgaatgcggc ctggacgcgg ccgagatcag gcgccgcaac
      181 ttcatccccg aatccgccca gccctaccag acgccggtgg cgatggtcta tgacggcggc
      241 aactacgcca agaacatggc ggactgcctg aaggccgccg acgccgccgg cttcgccgcc
      301 cggcgcaagg acgccaaggc gcgcggcaag ctgcgcggct ttggcctggc ctgctacatc
      361 gaggcctgcg gcgcggcgcc gtcggcgatc gccggcgcgc tcggcgcgcg cgcggggctg
      421 tacgagatgg ccaccgtgcg cgtcaacccg accggctcgg tgaccgtgat caccggcacc
      481 cacagccacg gccagagcca cgagaccacc tttgcccagc tggtttccga gcgcctgtcg
      541 ctgccgctgg aggcgatcga ggtggtgcac ggcgacaccg accgcatccc gttcggcatg
      601 ggcacctacg gctcgcgctc gctggcggtg ggcggcagcg cgctggtcaa gtcgctcgac
      661 aaggtggtgg acaagggcaa gcgcatcgcc ggccacatcc tcgaggccgc ggccgacgac
      721 atcgagttcg ccaacggccg cttcaccgtc tcgggcaccg accgctcgct cgctttcgcc
      781 gagatcgccg gcgccgccta cgtgccgcac aactatccgc tcgccgagct cgaaccgggg
      841 ctcgaggaga cctcgtattt cgatccgccc aatttcacct tcccgagcgg ctgctacgtg
      901 tgcgaggtcg aggtcgaccc cgacacgggg acggtgtcga tcgagcgctt caccgcggtc
      961 gacgacttcg gcgtggtgat caaccccaac gtcgtcgagg gccaggtcca cggcggcctg
     1021 gtccagggca tcggccaggc gctgtacgag ggctgccgct acgaccccga gaacggccag
     1081 ctgatcaccg gctcgtacat ggactactgc atgcccaagg caagcgacgt gccgccgttc
     1141 gcggtctcga ccaacgtcac cttgagcccg accaacccga tgggcgtgcg cggctgcggc
     1201 gaggccggcg cgatcggcgc gccgccagcg gtgatcaacg ccgtcgtcga cgccttgtcg
     1261 gactacggcg tcacccacat cgacatgccg gcgacgccgc agcgggtgtg gcggacgatc
     1321 cagggccacg cgcacgcccg ggcggccgag tagagggagg cagcgatcga tgtacgactt
     1381 cacctatcac cgcgcggcga gcgtcgacca agccaagaag ctgctcgccg ccgccgccga
     1441 cgggcgctac ctcgccggcg gcatgacctt gctgccgacc atgaagcagc ggctggcgaa
     1501 cccgagcgac ctgatcgacc tcgccggcat cgccgagctc gccggcatcc gccgcgacgg
     1561 caaggcgctg gtggtcggcg ccatgaccac ccacgccgcg gtcgcggcca gcgccgaggc
     1621 gcgcaaggcg atccccgcgc tcgccgcgct cgccggcatg atcggcgacc cgcaggtgcg
     1681 caaccgcggc accatcggcg gctcgctggc caacagcgac cccgccgccg actacccggc
     1741 cgcggtgctg gcgctgggcg cgaccgtgcg caccgaccgg cgcgagatcg ccggcgacga
     1801 cttcttcacc ggcatgttcg agaccgcgct cgataacggc gagctgatca ccgcggtgtc
     1861 gttcccggtg cccgaggcgg cctgctacat gaagttcgcc aacccggcct cgcgctacgc
     1921 catcgtcggc gtgatggtgg cgcgcaccgg cgcaggcgtg cgggtggcgg tgaccggcgc
     1981 cgggccgtgc gcgttccggg ttgccgacat ggagaaggcg ctggccaaga agttcgcgcc
     2041 cgaggcgatc gcgggcatcg gcgtgccggc cgacgacctc aacgccgaca tccatgccgg
     2101 cgccgactac cgcgcccatc tgatcggggt gatggccaag cgggcggtcg cggggtgcgc
     2161 ctgagccggg cttggcgatg acggcgctgc cggcttcggt cgacgaggcg ctggcgctgc
     2221 tcgccgcggg gcactacgtc gccgaccgca gcctcgccac cgcgctctat ctgtcgctgt
     2281 ccatgggccg gccgctgttc ctcgagggcg aggccggggt cggcaagacc gagatcgcca
     2341 aggtgctcgc cgagcggctc gaccggcgcc tggtgcggct ccagtgctac gagggcctcg
     2401 acatagccag cgcggtctat gagtggaact acgccgccca gatggtcgag atccaggccg
     2461 ccaaggcgga agggatcgcc gaccgcgcgc gcctgatcga cgacgtgttc tcgcgccggt
     2521 tcctgatcga gcggccgatc ctgcaggcgc tcgagccgga cagtcgcggc gcgccggtgc
     2581 tcttgatcga cgagctcgac cgcgccgacg agccgttcga ggcctacctg ctcgaggtgc
     2641 tcgccgactt ccaggtcacc gtgcccgaga tcggcaccat ccgcgcgccg agtgcgccga
     2701 tcgtggtcat cacctcgaac cgcacgcgcg agatccacga cgcgatcaag cgccgctgct
     2761 tctacttctg gatcgactat cccgacgccc ggcgcgagaa gcagatcctg gccgcgcgcg
     2821 tgcccggcgc gcccgagcgg ctgtcgggcg aggcggtggc gttcgtgcag cggctgcgcg
     2881 ccctcgacct gttcaagaag ccgggcgtcg ccgagaccat cgactgggcg aacgcgctga
     2941 tgcgcctcga cgcgatcgag ctcgacgccg agaccgtcga tgcgaccctc ggcgtgctgc
     3001 tcaaatacca ggacgacatc gcgcgcctgc gcggcaccga ggccgccgcc atcctcgacg
     3061 agatccgccg cggcagcgag ggcgcgcgcg cgcgcgccgc cggcgccggc gcatgagcgg
     3121 gctcaggctg atcgacctcg agggcgacgc cgaggcgcgc gggcaagccc acggccgggc
     3181 gctcaagtcc gccatcgccg acaatgtcga gacctacctc gcccgcttcg agtcgggcgg
     3241 catcgcccgc gccgacgcgc ttgccgaggg cgcgcgctgg ggcgagcgca tcgcgcgctt
     3301 cgatgaagat tacgcggccg agatgcgcgc gatcgccgag gcggcgggca tcgatgccgc
     3361 ggcggtggcg atgctgaacg cgcgctggga gttgagctac ggcatcaccg cgcgcgacgc
     3421 gctcgcccgc gacggctgca ccgcgttcgc ggcgctgccc gagatcacgc gcaccggcac
     3481 caccttgatc ggccagaact gggactggct cgagcgcctg cgcggccgcc tcgccgtgct
     3541 gcgcgtgcgc ggccgcggcc atcccgactt cctgtgcatc acccaggccg gcatcgccgg
     3601 cggcatgatc gggctcaatg ccgccggcat cgggctgtgc gtcaacggca tgacctcgga
     3661 ccgcgacggc cgcgacccgg agcgcaagcc gttccacctg cgcgtgcgcg aggcgctgcg
     3721 cgcgtcctcg ctcgccaccg cgctcaaggc gttcctcggc agcccgcgcg cctgctcggt
     3781 caacgtcctg ttcggccacg ccggcggcga ggcgatcgac gtcgaggcgg cgcccgacac
     3841 cgaaagcgtg ctctaccccg agggcggcct gatcacccac gccaaccatt tcgagcgccc
     3901 gcccggggtg gtctcgaccc tgcaatggct gcgcccgggc acgctctacc gcgcgcgccg
     3961 gctgcgccgc ctgttccgcc aggccaaccg ggcggtcgac gtcgagctcg cccaagagct
     4021 gttcgccgac cacttcagcc ggccgcactc gatttgtgcc cacccggatg cggacgtgga
     4081 cgagatcgag cgcaccacca ccgtcacctc ggcgatcctc gacctcggca accgggtgct
     4141 ctacgccacc gacggcccgc cctgcgagaa cccgtaccgg gcctatccgc tcgaccgatg
     4201 accgcgcccg cccgcggccc cgggcatctc gccgacaacg tcatgcattt ctgccgcacc
     4261 ttgcgcgccg ccgggctgcc ggtggggccg ggcaaggtga tcgacgcgct gggcgcgatg
     4321 gcgcgcaccg ggctcgcccg gcgcgacgac gtctactggg cgctccatgc cagcctggtc
     4381 aaccggcgcg accagcacga gctgttcgac caggccttcc atgcgttctg gcgcgcgccc
     4441 gacctgctgg agcggctcgc gggcctcgcg ctgcccgagt tcgtgcgcgt ggccaaggcg
     4501 ccgccggccg cgcgcagggt ggccgaggcg ctgcgcgctg tgcgcggcga gggcgaggag
     4561 cgcgcgcccg agacccgcga gctcgacgcc gcgctcaccg cctcgggcgc cgaggtgttg
     4621 cagaagatcg acttcgagcg catgaccgcg gacgagatcg cgctcgccaa gcgcgcgatc
     4681 cgcgagctcg cactgtcgat cgcggcgctg ccgacgcgcc gcttcgcgcc ggcgacgcac
     4741 ggcgcccgca tccacatgcg ccggaccttg cgcgcggcgc tgcgcggcca aggcgaggcg
     4801 atcccgctgg tccgccgccg gcggctgcgc cggccgccgc cgatcgtcgt gctgtgcgat
     4861 atctcgggct cgatggggcg ctattcgcgc atgctgctgc acttcctgca cgcgctcgcc
     4921 aacgaccgcg accgggtgca cgtgttcgcc ttcggcaccc ggctgaccaa cgtcacccgc
     4981 aggcttcgcc agcgcgacgt cgacgtggcc atggacgcgg tggtggacgc ggtcgaggac
     5041 tggtcgggcg gcacccgcat cggcgactgc ctgcacgact tcaaccgcgc ctggtcgcgg
     5101 cgcgtgctcg gccagggcgc gatcgtgctg ttgatctcgg acggcctcga ccgcgcggcg
     5161 ggggtcgacc tggggcgcga gatggagcgg ctgcacaagt cgtgccggcg gctgatctgg
     5221 ctcaacccgc ttttgcgcta tgatggcttc gagccgaagg cgctcggcgt gcgcgcgatg
     5281 ctgccgcatg tcgacgagtt ccggccggtg cacaacctgg agaccatcgc cgcgct
//
LOCUS       DASWHU010000197         7131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3414588, whole genome shotgun sequence.
ACCESSION   DASWHU010000197 DASWHU010000000
VERSION     DASWHU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3414588"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1455)
                     /locus_tag="VGB88_07105"
     CDS             complement(142..1455)
                     /locus_tag="VGB88_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647250.1"
                     /translation="MTGSTSLTRGVRAVAAGAVVAGAVAAAGVAHAKDTVTIGLVAFI
                     SGPAAGVFGIPAQNGAEVIIDALNAGEMAPPYDTPGIAGAKIEVQIVDEAGGATKQVA
                     EFRNMVQRQGVDLVLGYISSGDCLAVPQVAEELKMLTVLADCGTPRVFEDASYKYVFR
                     TGPHAVMDNVAAARYLLAMRPEVKRIAGINQNYAWGQDSWTDFSTSMEVLQPGVEVVT
                     AQFPKIFAGQYGAEISTLLATRPEVVHSSLWGADLEAFILQAGGRGLFDRSTVILSAG
                     EQILNSIGAQLPDGTIIGGRGPHGPFAPKSALNDWFQARYVERVGNVPNYASYKYAQA
                     ILGVKLAYEKAAAAAGGWPTPEQVIAAFEHLEYTGPSGPVMMRLGNGHQAVQANAIGR
                     SKWDPGLGRVTVTDVTYYDADCVNPPEGTTGIEWMKAGFPGAKCN"
     gene            1577..2395
                     /locus_tag="VGB88_07110"
     CDS             1577..2395
                     /locus_tag="VGB88_07110"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX9647251.1"
                     /translation="MSRLRTRTPPLFDPEFLDSAAARPIRFLSEYLEPEDRLARLGVR
                     DTVVFLGSSRIPEPAQAEAALERARRGGGDVAEAERMVAMSAFHDRARELARRLTEWS
                     RAQAEDGHRFVVCTGGGPGLMASANLGACDAGGPTIGLNIEIPGLQPVNPHVPAELSF
                     EFRYFFMRKFWFVYMAKAIVVLPGGFGTLDEFFEVLTLKQTGKVAGPLPIVLLGAEFW
                     RDVVDFEALVRYGTVRRADLALFRVTDSVDQAFDFLTAELGRRPLVTPGPAMAP"
     gene            2427..3479
                     /locus_tag="VGB88_07115"
     CDS             2427..3479
                     /locus_tag="VGB88_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX9647252.1"
                     /translation="MRSYQIVEWDRPLALAERATPAPQGTEVLLKVDACGVCHSDIHI
                     RQGYFDLGDGERIRLADRGARLPLTMGHEVVGEVAALGPEAAGARVGDKRIVFPWIGC
                     RQCEVCLTGKEMLCLTPKFIGARVDGGYSDYVLVPHPKYLIDYAGVATDLACTYACSG
                     ITAYSALRKVAPLGATDTVLIVGAGGVGLNGVYIARALYESRLVVADIDAAKRAAAED
                     AGADHVIDNSAPGALDQVIELSGGGVAAALDFVGAPATSKFGFDSLRKGATLVVVGLY
                     GGKLPVPLPLMPQKALTVRGSYVGTLAEMHALMELVRAGRVPPLPVHARRLDEAEQAL
                     ADLASGRVVGRYVLKP"
     gene            complement(3493..4635)
                     /locus_tag="VGB88_07120"
     CDS             complement(3493..4635)
                     /locus_tag="VGB88_07120"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEX9647253.1"
                     /translation="MAQETVFTNARVVGPDSVFAGTVVVRGADIAAVEDGPSRHASAL
                     DLDGDYLVPGLVELHTDNLEKHVVPRPGVRWPTAAAAVAHDAQVVAAGITTVFDALVV
                     GDIRADSARRRDLVDMAETTSRAQAAGMLRAEHFLHMRCEVGCANLFELYQPFVDHPL
                     VGLVSVMDHTPGQRQFTDDRQYRAYYKEKWGTTDAEMEAFIAGQKEVAHRHAGPNRAR
                     VAALCRARGLPLASHDDATEAHIDEAAGLGVALAEFPTTLGAARAARARGLKVIAGAP
                     NLVRGGSHSGNVSVQELAEADLVDILSSDYMPVSLLHGAFLIAGSGEGSLPAAIAAVS
                     LTPARAAGLDDRGEIAPGLAADLVRVRIVEALPLVRAVWRRGARIG"
     gene            4756..>7131
                     /locus_tag="VGB88_07125"
     CDS             4756..>7131
                     /locus_tag="VGB88_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9647254.1"
                     /translation="MIITCPSCAFDNPDHARFCGNCGISLALTCGACGHANAPSYNFC
                     INCGTPLGARAPAVLPAPVAAAASAGWPVPAVAAEAGGTSWPVIADLGPERRHLTVLF
                     ADLVGSTELAQELDPEDLRVVMRAYETASSEVIERFGGYVAQYYGDGVLAYFGYPIAH
                     EDAAERAVRAALALIEAVAQTRAPQPLRVRIGIHSGLVVVGMEKTGVPGKANSAVGET
                     PNVAARLQALADPNTVLISAATSQLVRGRFVVEDAGAHALKGIGAEVQVFRVVGEIDG
                     GRRQDRELGGELVPLVGRARDLEAILDRWVKARHGDGQVVLVSGEAGIGKSRLVHELR
                     ARLHTEPHILMRHHCSPYHQTSPLFPIAEAIAGAAGIDSGDAPAARLAKIGHWLERYD
                     IAAEESLALVADLLMVPSEDGRPPLAMAPEQRKQRIFALLLAAVESAARDAPVLFEVG
                     DLQWADPSTIEFLGLIVDQVATRRVLALLTHRPGFQPPWPARAHVLPLTLARLTAAES
                     AAMAEHVAGGKALPQAVLDRIVERGDGVPMFIEELTRTVLASGFLEERDGRYELAGEL
                     PPVAIPASLQDSLMARLDRLGAYKEVAQIGAMLGRSFRADVLAAVSGLDADTLGEALA
                     ALVAGELLLQRGLPPHASYVFRQALVQEVAYQSLLRSRRQALHKQIAEVIEARFPDVA
                     EAEPEALARHYQAAGVTDRALDYGRRASERAIRASAMKEALAHADGALALVSAMAPGS
                     DVRRAEMELQVMRGIALGAVNGFGDPAVRTAYGRALEISRETGEREFEFRAL"
BASE COUNT          945 a         2551 c         2595 g         1040 t
ORIGIN      
        1 gattgagtca cgacaggtcc tcgtcctgag gagcgagcga agcgagcgtc tcgaaggacg
       61 acagccggtt cattgacacg ctcacggtcg ggccgacagg cgcgctgcgc ttgccggccg
      121 cttgtttgct tgcctaactg cctagttgca cttagcgccg gggaagccgg ccttcatcca
      181 ctcgatgccg gtggtgcctt cgggcgggtt gacgcagtcg gcgtcgtagt aggtgacgtc
      241 ggtcaccgtc acccggccga gaccggggtc ccacttgctg cggccgatgg cattcgcctg
      301 gaccgcctga tggccgttgc cgagccgcat catcaccggc ccgctcggcc cggtatattc
      361 gaggtgctcg aaggccgcga tcacctgctc cggcgtcggc cagccgccgg ccgcggccgc
      421 cgccttctcg taggcgagct tcacgccgag gatcgcctgg gcgtacttgt acgaggcgta
      481 gttgggcacg ttgccgacgc gctcgacata gcgcgcctgg aaccagtcat tgagcgcgct
      541 cttaggggcg aacggcccgt gcgggccgcg gccgccgatg atcgtgccgt cgggaagctg
      601 cgcgccgatc gagttcagga tctgctcgcc cgcgctcagg atcaccgtcg agcggtcgaa
      661 caggccgcga ccgcccgcct ggaggatgaa cgcctcgagg tcggcgcccc acaggctgga
      721 gtggacgacc tcggggcggg tggcgagcag ggtcgagatc tcggcgccgt actggccggc
      781 gaagatcttc gggaactgcg cggtgacgac ctcgactccc ggctgcaata cctccatcga
      841 ggtcgagaag tcggtccacg agtcctggcc ccaggcgtag ttctggttga tgccggcgat
      901 gcgcttgacc tccggcctca tcgccagcag gtagcgggca gcggcgacgt tgtccatcac
      961 cgcgtgcggg ccggtgcgga acacgtactt gtagcttgcg tcctcgaaca ccctgggcgt
     1021 gccgcagtcg gcgagcacgg tcagcatctt gagctcttcc gcgacctggg gcaccgccag
     1081 gcagtcgccg ctcgagatgt agccgagcac caagtcgacg ccctggcgct ggaccatgtt
     1141 gcggaactcg gcgacctgct tggtggcgcc gccggcctcg tcgacgatct ggacctcgat
     1201 cttggcgccg gcgatgccgg gggtgtcgta gggcggcgcc atctcgccgg cattgagcgc
     1261 atcgatgatc acctcggcgc cgttctgcgc cggaatcccg aacacgcccg ccgccgggcc
     1321 ggaaatgaac gccaccaggc cgatggtgac ggtgtccttg gcgtgcgcga cgccggcggc
     1381 cgcgaccgcc cccgccacca ccgcgccggc cgcgaccgcg cgcacgccgc gcgtcaagct
     1441 ggtgctgcct gtcatcgctg attcctccct cagcccgagc cgtaccgggt tcggggcgag
     1501 gtttagcgca tttgccgccg caggggcaat ctgtgtcaaa ctcgccgcga gccccggcca
     1561 gccgaggata cgactgatgt cacgcctgcg aacccgcacc ccgcccctgt tcgaccccga
     1621 gttcctggac agcgcggcgg cgcgtccgat ccgcttcctg tccgaatacc tggagcccga
     1681 ggatcggctg gcgcggctcg gcgtgcgcga caccgtggtg ttcctgggct cgtcgcgaat
     1741 tcccgagccg gcgcaggccg aggccgcgct cgagcgcgcc cgccggggcg gcggcgacgt
     1801 ggccgaggcc gagcggatgg tggcgatgtc cgcgttccac gaccgcgcgc gcgagctcgc
     1861 ccggcggctc accgaatggt cgcgcgcgca ggccgaggac ggccaccgct tcgtcgtctg
     1921 caccggcggc ggccccggcc tgatggcgtc ggccaatctc ggcgcgtgcg acgccggcgg
     1981 cccgaccatc ggcctcaaca tcgagatccc gggcctccag cccgtcaacc cgcacgtgcc
     2041 ggccgagctg tcgttcgagt tccgctactt cttcatgcgc aagttctggt tcgtctacat
     2101 ggccaaggcg atcgtggtgc tgcccggcgg cttcggcacc ctcgacgagt tcttcgaggt
     2161 gctgaccttg aagcagaccg gcaaggtcgc ggggccgctg ccgatcgtgc tgctgggcgc
     2221 ggagttctgg cgggacgtgg tcgacttcga ggcgctggtc cgctacggca ccgtgcggcg
     2281 cgccgacctg gcgctgttcc gggtgaccga ttccgtcgac caggcgttcg acttcctgac
     2341 cgccgagctc gggcggaggc cgctggtcac gcccggcccg gcgatggcac cctgagcgcg
     2401 caacaaccga ggaggcgtga gcgaccatgc gcagctatca gatcgtcgag tgggaccggc
     2461 cgctggcgct cgccgagcgc gccacgcctg ccccccaggg caccgaggtg ctgctcaagg
     2521 tcgacgcctg cggcgtctgc cacagcgaca tccacatccg ccagggctat ttcgacctcg
     2581 gcgacggcga gcgcatccgg ctcgccgacc gcggcgcgcg cctgcctttg accatgggcc
     2641 acgaggtggt cggcgaggtg gcggcgctcg gccccgaggc cgccggcgcc agggtcggcg
     2701 acaagcgcat cgtgttcccg tggatcggct gccggcagtg cgaggtgtgc ctcacgggca
     2761 aagagatgct gtgcctgacg cccaagttca tcggcgcgcg cgtcgacggc ggctacagcg
     2821 actacgtgct ggtgccgcac cccaagtacc tgatcgacta tgcgggggtt gccaccgacc
     2881 tcgcctgcac ctatgcctgc tcgggaatca ccgcctacag cgcgctcagg aaggtggcgc
     2941 cgctcggggc cacggacacc gtcctgatcg tcggcgcggg cggggtcggg ctgaacggcg
     3001 tctacatcgc gcgcgcgctc tatgagtcgc gcctggtggt cgccgacatc gatgcggcca
     3061 agcgcgccgc ggccgaggac gccggcgccg accacgtgat cgacaactcg gcgcccggcg
     3121 cgctcgatca ggtgatcgag ctgtcgggcg gcggggtggc cgcggcgctc gatttcgtcg
     3181 gcgcgccggc gacctccaag ttcgggttcg attcgctgcg caagggcgcg accctcgtcg
     3241 tggtcgggct ctacggcggc aagctgcccg tgccgctgcc gctgatgccg cagaaggcgc
     3301 tgacggtgcg cggctcctat gtcggcacgc ttgccgagat gcacgcattg atggagctgg
     3361 tgcgcgccgg ccgggtgccg ccgctgccgg tccacgcccg ccggctcgac gaggccgagc
     3421 aggcgctcgc cgacctcgcc tccggccgcg tcgtcggccg ctacgtgctc aagccgtgag
     3481 tgcgctcgcc gttcatccga tgcgcgcgcc gcgccgccag accgcgcgca ccagcggcag
     3541 cgcctcgacg atgcgcaccc gcaccaggtc cgcggccagg ccgggggcga tctcgccgcg
     3601 gtcgtcgagc ccggcggcgc gcgccggcgt caggctgacc gccgcgatcg ccgcgggcaa
     3661 cgacccctcg ccgctgccgg cgatcaggaa cgcgccgtgc agcaggctca cgggcatgta
     3721 gtccgacgac aggatgtcga ccaggtcggc ctcggccagc tcctgcaccg agacgttgcc
     3781 cgaatgcgag ccgccgcgga ccaggttggg cgcgccggcg atcaccttga gcccgcgggc
     3841 gcgggcggcg cgggcggcgc cgagcgtggt cgggaactcg gccagcgcga cgccgagccc
     3901 cgcggcctcg tcgatatgcg cctcggtggc gtcgtcatgg ctcgccagcg gcagcccgcg
     3961 tgcccggcac agcgcggcga cgcgcgcgcg gttgggcccg gcgtggcggt gcgcgacttc
     4021 cttctggccg gcgatgaacg cctccatctc ggcgtccgtg gtgccccatt tctccttgta
     4081 ataggcgcga tactggcggt cgtcggtgaa ctggcgctgg cccggggtgt ggtccatcac
     4141 cgacaccaga ccgaccagcg ggtggtcgac gaacggctga tacagctcga acaggttggc
     4201 gcagccgacc tcgcagcgca tgtgcaggaa gtgctcggcg cgcagcatgc cggcggcctg
     4261 cgcgcgcgag gtcgtctccg ccatgtcgac caggtcgcgc cggcgcgcgc tgtcggcgcg
     4321 gatgtcgccg acgacgaggg cgtcgaacac ggtggtgatt cccgccgcca ccacctgggc
     4381 gtcgtgggcg accgccgccg ccgcggtcgg ccagcgcaca ccggggcgcg gcaccacgtg
     4441 cttctccagg ttgtcggtgt gcagctcgac cagccccggc accaggtaat cgccgtcgag
     4501 gtccagcgcc gatgcgtgcc tggacgggcc gtcctcaacc gccgcgatgt ccgcgccgcg
     4561 caccaccacg gtaccggcga agaccgaatc gggcccgacc acgcgcgcat tggtgaacac
     4621 ggtttcctgg gccatggcga agggctttcg ctcctcccgg agtggaccgg gcgccgagtc
     4681 tagcatggcc gggggcggcg gaaagccggc gccgctgttg ccttcccggc ccggcgggtt
     4741 cacatatgaa ccgccgtgat catcacctgt cccagctgcg ctttcgacaa cccggaccac
     4801 gcccggttct gcggcaactg cggcatttcg ctggccctga cctgcggcgc ctgcgggcac
     4861 gcgaatgcgc cgagctacaa cttctgcatc aactgcggga cgccgcttgg cgcgcgcgcg
     4921 ccggccgttt tgcccgcgcc ggtggccgcg gccgcctcgg caggctggcc tgtgccggct
     4981 gtcgctgccg aggccggcgg caccagctgg ccggtgatcg ccgacctcgg gcccgagcgc
     5041 cgccacctga cggtgctgtt cgccgacctg gtcggctcga ccgagctcgc ccaggagctc
     5101 gatccggagg acctgcgcgt ggtcatgcgc gcctacgaga ccgcctcgag cgaggtgatc
     5161 gagcgcttcg gcggctacgt cgcgcagtat tacggcgacg gcgtgctcgc ctatttcggc
     5221 tacccgatag cgcacgagga cgccgccgag cgcgcggtgc gcgcggcgct ggcgctgatc
     5281 gaggcggtgg cccagacgcg cgcgccccaa ccgctgcgcg tgcgcatcgg catccattcg
     5341 gggctggtcg tggtcggcat ggagaagacc ggcgttcccg gcaaggccaa cagcgcggtc
     5401 ggcgagacac cgaacgtcgc cgcccggctg caggcgctcg ccgacccgaa cacggtgttg
     5461 atcagcgccg cgaccagcca gctggtgcgc ggccgcttcg tggtcgagga cgccggcgcg
     5521 catgcgctca aggggatcgg cgccgaggtg caggtgttcc gcgtggtcgg cgagatcgac
     5581 ggcggccggc gccaggatcg cgagctgggc ggcgagctgg tgccgctggt cgggcgcgcg
     5641 cgcgacctcg aggcgatcct cgaccgctgg gtcaaggccc gccacggcga cggccaagtg
     5701 gtgctggtca gcggcgaggc cgggatcggc aagtcgcggc tggtccatga gctgcgcgcg
     5761 cgcctgcaca ccgagccgca catcctgatg cgccatcact gctcgcccta ccaccagacc
     5821 agcccgctgt tcccgatcgc cgaggcgatc gcgggcgcgg ccgggatcga ctcgggcgat
     5881 gcgccggcgg cgcgcctggc caagatcggg cactggctgg agcgttacga catcgccgcg
     5941 gaggagtcgc tggcgctggt cgccgacctg ctgatggtcc ccagcgagga cggcaggccg
     6001 ccgctcgcca tggcccccga gcagcgcaag cagcgcatat tcgcgctgct gctggcggcg
     6061 gtggaaagcg cggcgcgcga cgccccggtg ctgttcgagg tcggcgacct gcaatgggcc
     6121 gatccgtcca cgatcgagtt cctcggcctg atcgtcgacc aggtggcgac ccggagggtg
     6181 ctcgcgctgc tgacccatcg gcccgggttc cagccgccct ggccggcgcg cgcgcatgtg
     6241 ctgccgctga cgctcgcccg gctgaccgcc gccgagtcgg cggcgatggc cgagcacgtc
     6301 gccggcggca aggcgctgcc ccaggcggtg ctggaccgga tcgtcgagcg cggcgacggg
     6361 gtgccgatgt tcatcgagga gctgacccgc accgtgctcg cctcgggctt cctcgaggag
     6421 cgcgacggcc gctacgagct ggccggcgag ctgccgccgg tggcgatccc ggcgagcctc
     6481 caggacagcc tgatggcgcg gctcgaccgg ctcggcgcct acaaggaggt ggcgcagatc
     6541 ggcgccatgc tcgggcgcag cttccgcgcc gacgtgctcg ccgcggtcag cggcctggac
     6601 gcggacacct tgggcgaagc gctcgcggcg ctggtcgcgg gcgagctcct gctccagcgc
     6661 ggcctgccgc cgcacgcgag ctacgtgttc cgccaggcgc tggtccagga ggtcgcttac
     6721 cagtcgctgc tcagaagccg gcgccaggcg cttcacaagc agatcgcgga ggtgatcgag
     6781 gcgcgcttcc cggacgtcgc cgaggccgag cccgaggcgc tggcgcggca ctatcaagcg
     6841 gccggggtca ccgaccgggc gctcgattac ggccgccggg cgagcgagcg cgcgattcgc
     6901 gcctcggcga tgaaagaggc gctcgcgcat gccgacggcg cgctggcgct ggtctcggcg
     6961 atggccccgg ggagcgacgt tcggcgcgct gagatggagc tgcaggtgat gcgcggcatc
     7021 gccctcggcg cggtcaacgg cttcggcgac cctgcggtgc gcacggccta cgggcgcgcg
     7081 ctcgagatca gccgcgaaac cggcgaaagg gagttcgagt tccgcgccct g
//
LOCUS       DASWHU010000198         5039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_97818, whole genome shotgun sequence.
ACCESSION   DASWHU010000198 DASWHU010000000
VERSION     DASWHU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97818"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGB88_07130"
     CDS             <1..373
                     /locus_tag="VGB88_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647255.1"
                     /translation="HRELLGQAIANLLDNAIKYTPAGGRIAIVLDRAGGAPRLAIADS
                     GPGVPAGERARVLERFVRLDSSRGTPGSGLGLSLVAAVAKLHGARLALADNAPGLSIT
                     LTFAGAAAAPRRAPAERMTAM"
     gene            470..790
                     /locus_tag="VGB88_07135"
     CDS             470..790
                     /locus_tag="VGB88_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647256.1"
                     /translation="MFTRLIVPAILGALVLSAPVLAAEKAATPSTPATQTAAKSTPGE
                     RCMALEQQFDAVIKDHGNAAKAGEAKTMRSEGGRLCTDGKHSDGIAKLEQGIRDLGVT
                     PVKG"
     gene            1011..1283
                     /locus_tag="VGB88_07140"
     CDS             1011..1283
                     /locus_tag="VGB88_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647257.1"
                     /translation="MRPDPKPLPIPIAPGARARPADPPAITPDLVDRYLRRGRRLHAE
                     QVWRVGRALAALVGGLPGAAARLVSRPRPAGVPGAKLGALWQAWSR"
     gene            1271..1762
                     /locus_tag="VGB88_07145"
     CDS             1271..1762
                     /locus_tag="VGB88_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647258.1"
                     /translation="MVKVNGRRRCAALLVGAALLVGAGCVTRLDFASGQPRPADATLV
                     EDLAGFVAGRYLLIGRDPQGRAYVGTLRLERAEGALSALRCVAGERLDGRAHIDFATA
                     DRVPVLDIDYGGAGGLDGARFVIDGDLDNSALLSGPVRWRGAPGRGWEHAHADPAAAD
                     ECG"
     gene            complement(1769..2272)
                     /locus_tag="VGB88_07150"
     CDS             complement(1769..2272)
                     /locus_tag="VGB88_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX9647259.1"
                     /translation="MAASAAGRLLLTLVLALAPLAARAVDAGSETGLPLPRFASLGTD
                     RVNVRTGPGTRYPVAWVFVREALPIEVVGEFEQWRRVRDHDGAEGWVHKAMLSGRRTV
                     VVTGGVRTLHAAPRPDSPPVARVEAGVLGRLDSCRNDWCEVAFAGYRGWMPAGEMWGA
                     EEISAVR"
     gene            2475..3461
                     /locus_tag="VGB88_07155"
     CDS             2475..3461
                     /locus_tag="VGB88_07155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9647260.1"
                     /translation="MPRPKPLVIVTRKLPEAIEARMMELFEAKLNDDDHPMSRDELIE
                     AVKAADVLVPTVTDRIGPSVIGQAGPRLKLIASFGTGVDHIDLAAAQARGITVTNAPG
                     VLTEDTADMTMALILAVPRRLAEGERLIRTGNWRGWGPTSMLGHRIWGKRLGIIGMGR
                     IGSAVARRARGFGLSIHYHNRHRAAPAREAELEATWWESLDQMLARTDIISVNCPHTP
                     ATYHLLSARRLELLRPHAYIVNVSRGEVIDEAALTRLLRAGRIAGAGLDVFEHEPAIN
                     PRLIELDNVVLLPHMGSATHEARIETGEKVLVNIRTFIDGHAPPDRVLESLV"
     gene            complement(3574..4026)
                     /locus_tag="VGB88_07160"
     CDS             complement(3574..4026)
                     /locus_tag="VGB88_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX9647261.1"
                     /translation="MTERAARRPDKLSVVVFAGNFEKVHYALAAACAAAAIDMPATLF
                     FTMGACRALLAADAAGAPGWHALSLERPGFADAAALDASMRARGVAGFEELIEACAAL
                     GVRVLACEMGLRAMGLDGAPLRADLKIEIGGLVTFLTDASADGAMLFV"
     gene            complement(4031..4825)
                     /gene="moeB"
                     /locus_tag="VGB88_07165"
     CDS             complement(4031..4825)
                     /gene="moeB"
                     /locus_tag="VGB88_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX9647262.1"
                     /translation="MEFSDEQVERYARHIILPEIGGAGQARLLRARVLVIGAGGLGSP
                     LILYLGAAGVGTIGVVDYDQVDLSNLQRQVLHATEAVGTPKVDSARAAVARINPEVDV
                     VPHYARIDAANALGLIEGYDLVVDGSDNFATRFLVNDACYLARKTLVSAAILRFEGQV
                     STFKAHLGGENPCYRCIFPSPPPPGLIPTCAEGGVLGALAGAVGSVQAIEVIKELLGI
                     GTSLSGYLLIYDGLGCTFRKVRVRPDPACALCGPKAAIRDLTSHAA"
     gene            complement(4833..>5039)
                     /locus_tag="VGB88_07170"
     CDS             complement(4833..>5039)
                     /locus_tag="VGB88_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX9647263.1"
                     /translation="IDADYRGEIGVVLVNHGGEPFTVRRGMRVAQLVVAPVSRLAWQE
                     VALLPDSGRGAGGFGSTGARAEGS"
BASE COUNT          714 a         1872 c         1826 g          627 t
ORIGIN      
        1 ccaccgcgag ctgctcggcc aggcgatcgc caacctgctc gacaacgcca tcaagtacac
       61 cccggcgggc gggcgcatcg cgatcgtgct cgatcgcgcc ggcggcgcgc cgcggctcgc
      121 catcgccgac agcggcccgg gagtgccggc gggcgagcgc gcgcgcgtcc tggagcgctt
      181 cgtgcgcctg gattcgagcc gcggcacgcc cggaagcggg ctcgggttga gcctggtcgc
      241 ggcggtcgcc aagctgcacg gcgcccggct tgcgctcgcc gacaacgcgc ccggcctcag
      301 catcacgctc accttcgccg gcgccgctgc ggccccgcgg cgcgcgccgg cagagcgcat
      361 gaccgcgatg taatccgccc gccacggcgc ggataccacc gccctgccag catccggctc
      421 ccgaggcgcc ccgatcccgg gacgccatct gcaaggaaag gcatgtcaca tgttcacgcg
      481 cttgatcgtt ccggcgatcc tgggcgccct ggtgctcagc gcgccggtgc tggcggccga
      541 gaaggctgcc accccgtcca cgccggcgac gcaaacggcg gcgaaatcga cgcccggcga
      601 gcggtgcatg gcgctcgagc agcagttcga cgcggtgatc aaggaccacg gcaacgccgc
      661 caaggccggc gaggcgaaga ccatgcgcag cgaaggcggc cggctgtgca ccgacggcaa
      721 gcattccgac ggcatcgcca agctggagca ggggatcagg gacctgggcg tcaccccggt
      781 caagggctga cgcaatctcg tcggtccggg caagagggag cgcggcgcca tcgccgcgct
      841 ccctcgggcg tgtccggacg gccacgcccg cgctcttttg cagcgcacca tttgattgaa
      901 aatgatcctg aaaccaatat gatgtattag gtgcgttgca ataagaaggt cacaaaggcc
      961 tcacctgacg ggaggaagat gctcgttgtc cgccatgacg gaggtcttcc atgcggcccg
     1021 accccaaacc ccttccgatc ccgatcgcgc cgggcgcccg cgcccggccg gccgatcccc
     1081 cggcgatcac ccccgacctc gttgaccgtt atctgcggcg cggccgccgg ctgcacgccg
     1141 agcaggtgtg gcgcgtcggg cgggcgctcg cggcgttggt tggcggcctg cccggcgccg
     1201 ccgcgcggct cgtttcgcgg ccgcggccgg cgggcgtgcc cggcgctaag ctgggcgcgc
     1261 tgtggcaggc atggtcaagg tgaacgggcg ccggcggtgc gcggcgctcc tggtcggggc
     1321 ggcgctcctc gtaggcgccg gctgcgtcac ccggctcgat ttcgccagcg gccagccgcg
     1381 gcctgcggac gcgaccctgg tcgaggacct ggcgggcttc gtggccggcc gctacctgct
     1441 gatcggccgc gacccgcagg gccgcgccta tgtcgggacg ctcaggctgg agcgcgccga
     1501 gggcgcgctc agcgcgctgc gctgcgtcgc cggcgagcgt ctcgacggcc gggcgcatat
     1561 cgacttcgcc accgccgatc gggtgccggt gctcgacatc gactatggcg gcgccggcgg
     1621 gctcgacggc gcgcgcttcg tgatcgacgg cgacctcgac aactctgccc tgctcagcgg
     1681 gccggtgcgc tggcggggcg ccccggggcg cggctgggag cacgcccatg ccgatccggc
     1741 ggcggccgac gagtgcggct gagcgggctc agcggacggc gctgatctcc tcggcgcccc
     1801 acatctcgcc ggcgggcatc cagccccggt agccggcgaa ggcgacctcg caccagtcgt
     1861 tgcggcacga atcgagccgg ccgagcaccc cggcctcgac ccgcgcgacc gggggcgaat
     1921 ccgggcgcgg cgcggcgtgc agggtgcgca cgccgccggt caccaccacc gtgcgccggc
     1981 cggacagcat cgccttgtgc acccagccct cggcgccgtc gtggtcgcgc acccgccgcc
     2041 actgctcgaa ctcgccgacc acctcgatcg gcagcgcctc gcgcacgaac acccaggcca
     2101 ccggatagcg cgtgccgggg ccggtgcgga cgttgacccg gtcggtgccg aggctggcga
     2161 agcgcggcag cggcaggccg gtctcgctgc cggcatcgac cgcccgggcg gcgagcggcg
     2221 cgagcgccaa gaccagggtc agcagcagcc ggccggcggc cgacgccgcc atgccgcgcc
     2281 ggtgccgcgc gccgattgga gcgttgcgcg atcgaaacca cattggttga cgtcgaacca
     2341 cttcgcctcg ccccactgtc atatagcgcc cgcgccggca tgggccagga cgcgcgcgcc
     2401 gggcgcacgt ttgagtcggt gacggggttt tgctaaacag gcgcaccgtt cccgcacggc
     2461 cggagccgcg ctcgatgccc cgtcccaagc cgttggtcat cgtcacccgc aagctgcccg
     2521 aggcgatcga ggcgcggatg atggagctgt tcgaggccaa gctcaacgac gacgaccacc
     2581 cgatgagccg ggacgagctg atcgaggcgg tcaaggcggc cgacgtgctg gtgccgaccg
     2641 tcaccgaccg catcgggccg agcgtgatcg ggcaggcggg accgcgcctc aagctgatcg
     2701 cgagcttcgg caccggcgtc gaccacatcg acctcgccgc ggcgcaggcg cgcggcatca
     2761 cggtcaccaa cgccccgggg gtgctgaccg aggacaccgc cgacatgacc atggccctga
     2821 tcctggcggt gccgcgccgc ctcgccgagg gcgagcgcct gatccggacc ggcaactggc
     2881 ggggctgggg gccgacctcg atgctcggcc accgcatctg gggcaagcgg ctcgggatca
     2941 tcggcatggg ccgcatcggc agcgcggtgg cgcggcgcgc gcgcggcttc gggctgtcga
     3001 tccactacca caaccgccac cgggcggcgc ccgcgcgcga ggccgagctc gaggcgacct
     3061 ggtgggagag cctcgaccag atgctcgcgc gcaccgacat catctcggtc aactgcccgc
     3121 acaccccggc gacctaccac ctgctgtcgg cgcgccggct cgagctgctg cgcccgcacg
     3181 cctacatcgt caacgtctcg cgcggcgagg tgatcgacga ggcggcgctg acgcggctgt
     3241 tgcgcgcggg ccgcatcgcc ggcgccggcc tcgacgtgtt cgagcacgag ccggcgatca
     3301 acccgcgcct gatcgagctc gacaacgtgg tcttgctgcc gcacatgggc tcggcgaccc
     3361 acgaggcgcg gatcgagacc ggcgagaagg tgctggtcaa catccgcacc ttcatcgacg
     3421 gccacgcgcc ccccgaccgc gtgctcgaaa gcctggtgta ggggcccccc tcctgcctcc
     3481 ccccgtaaac ggggcgaggt accagaaatc aaggccctcc cccgctggcg ggggagggtt
     3541 gggtgggggt ggggcggcgc ctgggaccga cccctaaacg aacagcatgg cgccgtcggc
     3601 cgaggcgtcg gtgaggaagg tgaccaggcc gccgatctcg atcttgaggt cggcgcgcag
     3661 cggcgcgccg tcgaggccca tcgcgcgcag gcccatctcg caggcgagca cccgcacccc
     3721 gagcgcggcg caggcctcga tgagctcctc gaagccggcg acaccgcgcg cgcgcatgct
     3781 cgcatcgagc gcggcggcgt cggcgaagcc cgggcgctcg aggctgagcg cgtgccagcc
     3841 gggcgcgccg gcggcatcgg cggcgaggag cgcgcggcac gcgcccatgg tgaagaacag
     3901 ggtcgccggc atgtcgatcg cggccgcggc gcacgccgcg gcgagggcgt agtggacctt
     3961 ctcgaaattg cccgcgaaca ccaccaccga cagcttgtcg gggcggcgcg cggctcgctc
     4021 ggtcatcgcc tcacgccgcg tggctggtca ggtcgcggat cgccgccttc ggcccgcaca
     4081 gcgcgcaggc cgggtccggg cgcacgcgca ccttgcggaa ggtgcagccc agcccgtcat
     4141 agatcagcag gtagccggac aggctggtgc cgatgccgag cagctccttg atcacctcga
     4201 tcgcctgcac cgagccgacc gcgccggcga gcgcgccgag cacgccgccc tcggcgcagg
     4261 tcgggatcag cccgggcggc ggcgggctgg ggaagatgca gcggtagcac gggttctccc
     4321 cgccgagatg ggccttgaac gtgctgacct ggccctcgaa gcgcaggatc gccgccgaca
     4381 ccaatgtctt gcgcgccagg tagcaggcgt cgttgaccag gaaccgggtg gcgaagttgt
     4441 ccgagccgtc gaccaccagg tcgtagccct cgatcagccc aagcgcgttg gcggcgtcga
     4501 tgcgcgcata atgcggcacc acatcgacct cggggttgat gcgcgccacc gcggcgcgcg
     4561 cgctgtccac cttgggcgtg cccactgcct cggtcgcgtg cagcacctgg cgctgcaggt
     4621 tggacaggtc gacctggtcg tagtcgacca cgccgatggt gcccaccccg gccgcgccca
     4681 ggtacaggat cagcggcgag ccgaggccgc cggcgccgat cacgagcacg cgcgcgcgca
     4741 gcagccgcgc ctggccggcg ccgccgatct cgggcaggat gatgtggcgg gcgtagcgct
     4801 cgacctgctc gtcgctgaac tccatcgccg ggctagctcc cctcggcccg ggcgccggtc
     4861 gagccgaagc cgcccgcgcc gcggccgctg tcgggcagca gcgccacctc ctgccaggcg
     4921 agccggctga ccggcgccac caccagctgg gcgacgcgca tgccgcggcg gacggtgaac
     4981 ggctcgccgc cgtggttgac cagcaccacg ccgatctcgc cgcggtagtc ggcatcgat
//
LOCUS       DASWHU010000199         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2332946, whole genome shotgun sequence.
ACCESSION   DASWHU010000199 DASWHU010000000
VERSION     DASWHU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332946"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGB88_07175"
     CDS             complement(<1..206)
                     /locus_tag="VGB88_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9647264.1"
                     /translation="MANERRFAMAAPIPLRADYGGRALRRLAKASKDANQTRRLLALA
                     AIYDGGRRAAAAEVGGVGLQVIRD"
     gene            complement(510..1682)
                     /gene="rnd"
                     /locus_tag="VGB88_07180"
     CDS             complement(510..1682)
                     /gene="rnd"
                     /locus_tag="VGB88_07180"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX9647265.1"
                     /translation="MPVITTTDALARACERLRGEPFLCLDTEFIRETTYWPRLCLVQL
                     AGPDGAAVAVDPMAADVDLAPLLDLLNDRRVLKVVHAARQDLEMFYHLSRRVPGPLFD
                     TQVAAMVCGFGEAVAYDRLVRKLAGATIDKSSRFTDWARRPLSERQIAYALADVEHLP
                     TIYRKLDRRLRETGRTAWVTEEIKVLSDPAIYALEPRDAWRRIKTRSANPRLLAVLRE
                     VAAEREAYAQKHDVPRQRVLRDEALVEIAAHETASREALLAIRGMSKGLAQGPLGEAL
                     IKAVARGLAVPEAASPSIERPAPAPRGVGPLVELMKVLLKMRCEENDVAVKLVASTAD
                     LERIAADDAADVPALKGWRRALFGDDALALKRGELALTSEGGRIALVELEAAQDPR"
     gene            complement(1704..2693)
                     /gene="araD1"
                     /locus_tag="VGB88_07185"
     CDS             complement(1704..2693)
                     /gene="araD1"
                     /locus_tag="VGB88_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HEX9647266.1"
                     /translation="MRLVQFETTGGARRVGVPEDGALRVVAGAERVRDLALEAVRTGA
                     ALADLVSGRLGDAAEDYDRALAERRVLVPVDHPDPAHCLVTGTGLSHLGSAQARDEMH
                     AKLASGAELSDSMKMFRAGLEGGKPPPGKIGAAPEWFYKGDGGALVACGRPLESPGFA
                     LDGGEEAEIAGIYVIDDAGRPRRIGFALANEFSDHVLERQNYLLLAHSKLRPCALGPE
                     LLLGELPASVAGSVRVLRGDRELWRAELLSGEDNMSHSLANLEHHHFRYRAFRRPGDL
                     HVHFFGAGVLSVQAGVRAEDGDVFEISAAGFGRALRNPIRVQADNPLVDVAPL"
     gene            2768..4840
                     /locus_tag="VGB88_07190"
     CDS             2768..4840
                     /locus_tag="VGB88_07190"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX9647267.1"
                     /translation="MTRRIGVWLAALALAAWAGGAQAAVERIEVLAESAFAGGAAFGA
                     VGSYRKITGRLHYVIDPAEAANARIADIAYAARDGDGMVRFAGDFMLLMPADATAGNR
                     RLLYEVGNRGSIGMLTFFNDAPPTNDPASAADAGSGFLLEQGYALLWSAWNWDVRPGE
                     GRAQITLPAALGDDGAPVTGLVSAEITVNEPTLSQPVAWGNARGYPALDLDQADARLT
                     KRAHQNDPRREIPRANWRFAREERGRLVPDAASVAVDGGFEPGLLYEVVYRAGPAVVV
                     GLGLAAIRDSLSYFRYAAAPANPLAGTLDHVIAFGISQSGRVINQMLLDGLHVDERGR
                     GAFDAALVHVAGAGKGSFNHRFAQTTRHPSPHQDHQYPADFFPFATVASGDPVTGEIG
                     DLLAGARGSGVVPKIFFTNTSGEYWTRAASLLHTDVTGSRDLGLAESARLYVFASGQH
                     GSWLLDNRLWVENCINPLDYRPAMRALLLRLDAWASEGRAPPASVYPRITEGTLGTVA
                     DYRAKFPAIPGLRLPLVNLRPPRLELGPRWSAQRIIDRVPPGFGPAYETRVPMPDGDG
                     IDLGGIRLPEVAAPLGTFTGWNLRREGFGATDMIGRWFGSFIPFAADEAARAQAGDPR
                     PSRAARYQSRADYVAGVGAAAEALAGEGFLRAAEVPQIAARLGAFYDRLHARAPRPGA
                     CAYIVPRS"
     gene            complement(4855..5649)
                     /locus_tag="VGB88_07195"
     CDS             complement(4855..5649)
                     /locus_tag="VGB88_07195"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HEX9647268.1"
                     /translation="MSETTTPATVPETWLTLFAFTPRGGVERLYGPVHTVTTFEAVIE
                     DSFGRAVEQPPIKLRSESFDATGALIERDRYDETGALVDRAVFGYDADGRAVERIHYD
                     PAGRAILRYLYSYDARGRLVEMTGLSPEGAVCAGLKRQYDEAGRLISVVEVRDRDGSV
                     RETRWRIAYDSGASAAAAHGEVREDGAVVERLVHRYDSSGNMVECARLDDDGSSQVRG
                     RLVYDPHSEHGNWVRRTTEHWLREFGEERWRPVRVTYRRITFYDTE"
BASE COUNT          783 a         2185 c         2031 g          816 t
ORIGIN      
        1 cagtcccgga tcacctgcag gccgaccccg ccaacctcgg ccgccgccgc ccgccggccg
       61 ccgtcgtaga tcgccgccag cgccaacagc cgacgcgtct ggttggcgtc cttcgaggcc
      121 ttcgccaacc gccgcaaggc ccgtccgcca tagtccgccc gcaggggaat cggtgccgcc
      181 atggcaaacc tcctctcgtt tgccatggtg aatcacatct caaagccaat gggaatcccc
      241 cgcgtgagtc cgagtcagcg cgaggtggta tgagccggcc gcgcgcgtta accggccctc
      301 ccgcggggct ggccatcgag ccgcaagggt gcccgccacg cgtcttcgtc actccggccg
      361 gagcggagcg gtacgggcag ggcaagggtg gaatcaagag ctcgttggta cgaggtgctc
      421 ggctccgaac cgtatgcgga ctatcgattg ccgaggcatg aacttcggtt tgtctacggt
      481 ctccgatgac cgccgccggc cgggcgcggt cagcgcgggt cttgggcggc ttccagctcg
      541 accagcgcga tccgcccgcc ctccgaggtc agcgccagct cgccgcgctt gagcgccagc
      601 gcgtcgtcgc cgaacagggc ccgccgccag cccttgagcg ccggcacgtc ggcagcgtcg
      661 tcggcagcga tgcgctcgag gtcggcggtg ctggcgacca gcttgaccgc gacgtcgttc
      721 tcctcgcagc gcatcttcag cagcaccttc atcagctcga ccagcgggcc gacgccgcgc
      781 ggcgccggcg ccggccgctc gatgctgggc gaggccgcct cgggcacggc aagcccgcgt
      841 gccaccgcct tgatcaacgc ctcgccgagc gggccctggg cgaggccctt gctcatgccg
      901 cggatggcga gcagcgcctc gcggctggcg gtctcgtggg cggcgatctc gaccagcgcc
      961 tcgtcgcgca gaacccgctg gcgcggcacg tcgtgcttct gggcgtaggc ctcgcgctcc
     1021 gccgccacct cgcgcaggac ggcgagcagg cgcgggttgg cgctgcgggt cttgatccgc
     1081 cgccaggcgt cgcgcggctc gagcgcatag atcgcgggat cggacagcac cttgatctcc
     1141 tcagtcaccc aggcggtgcg cccggtctcg cgcaaccggc ggtcgagctt gcgatagatc
     1201 gtcggcaggt gctcgacgtc ggccagcgca taggcgatct ggcgctcgct cagcggccgc
     1261 cgcgcccagt cggtgaagcg cgaggacttg tcgatggtgg cgccggcgag cttgcgcacc
     1321 agccggtcat aggccaccgc ctcgccgaag ccgcacacca tcgccgcgac ctgggtgtcg
     1381 aacagcggcc ccggcacccg ccgcgacagg tggtagaaca tctccaggtc ctggcgcgcg
     1441 gcgtgcacca ccttgagcac tcgccggtcg ttgagcaggt cgagcagcgg cgcgaggtcg
     1501 acgtcagcgg ccatcgggtc gaccgccacc gccgcgccgt cgggcccggc gagctgcacc
     1561 aggcacaggc gcggccaata ggtggtctcg cggatgaact cggtatccag gcacaggaac
     1621 ggctcgcccc gcaggcgctc gcacgccctg gcgagcgcgt cggtggtggt gatcacgggc
     1681 atcgcgctgc tatagcactg ccgctagagc ggcgcgacgt cgaccagcgg gttgtcggcc
     1741 tgcacgcgga tcgggttgcg caacgcgcgc ccgaagccgg cggccgagat ctcgaacacg
     1801 tcgccgtcct cggcgcgcac gccggcctgg acgctgagta ccccggcgcc gaagaaatgg
     1861 acgtgcaggt cgcccgggcg gcggaaggcg cggtacctga aatggtggtg ctcgaggttg
     1921 gcgagcgaat gcgacatgtt gtcctcgccg ctcagcagct ccgcccgcca cagctcccga
     1981 tcgccgcgca gcacccgcac gctgccggcg acgctcgccg gcagctcgcc gagcaggagc
     2041 tcggggccga gcgcgcacgg gcgcagcttg gagtgggcga gcagcaggta gttctggcgc
     2101 tcgagcacgt ggtccgagaa ctcgttagcg agcgcgaagc cgatccgccg cggccggccc
     2161 gcgtcgtcga tcacatagat gccggcaatc tccgcctcct cgccgccgtc gagcgcgaag
     2221 ccgggagact cgagcggccg gccgcaggcg acgagcgcgc cgccgtcgcc cttgtagaac
     2281 cattccggcg cggcgccgat cttgcccggc ggcggcttgc cgccctcgag cccggcccgg
     2341 aacatcttca tcgaatcgct gagctcggcg ccgctcgcga gcttggcgtg catctcgtcg
     2401 cgcgcctggg cgctgccgag atgcgacagg ccggtgccgg tgaccaggca atgggccggg
     2461 tcgggatggt cgaccggcac cagcacccgc cgctcggcca gcgcccggtc gtagtcctcg
     2521 gccgcgtcgc cgagccggcc gctgaccagg tcggcgaggg ccgccccagt ccgcaccgcc
     2581 tcgagcgcca ggtcgcggac gcgctcggcg ccggcgacga cgcgcagcgc gccgtcctcg
     2641 ggcacgccga cccggcgcgc gccgcccgtg gtctcgaact ggacaagcct catcgccgtt
     2701 cctcccgccg cgcccgatcg ggcggttata ctcgcggctt ggcggatcgt gaacaaggga
     2761 gggccgcgtg accaggcgga tcggggtttg gctggcggcg ctcgcgctcg ccgcctgggc
     2821 gggcggcgcg caagcggcgg tcgagcggat cgaggtgctc gccgagtcgg cgttcgccgg
     2881 cggcgcggcg ttcggcgccg tcggctcgta ccgcaagatc accgggcggc tccattacgt
     2941 gatcgacccg gccgaggcgg cgaacgcgcg catcgccgat atcgcttacg ccgcgcgtga
     3001 cggcgacggc atggtccggt tcgccggcga cttcatgctg ctgatgcccg cggatgcgac
     3061 cgccggcaac cgccgcctgc tgtacgaggt cggcaaccgc ggcagcatcg gcatgctcac
     3121 cttcttcaac gacgcgccgc ccaccaacga cccggcgagc gccgccgatg ccggctccgg
     3181 cttcctgctc gaacagggct acgcgctgct gtggagcgcg tggaactggg acgtgcggcc
     3241 gggcgagggg cgggcgcaga tcacgctgcc ggccgccctc ggcgacgacg gcgcgccagt
     3301 caccggcctg gtctcggcgg agatcacggt caacgagccg acactcagcc agccggtagc
     3361 ctggggcaac gcgcgcggct acccggcgct cgacctcgac caggccgacg ccaggctgac
     3421 gaagcgcgcg catcagaatg atccgcgccg cgagatcccg cgcgccaatt ggcgcttcgc
     3481 gcgcgaggaa cggggccggc tggtgcccga tgccgcctcc gtcgccgtcg acggcggctt
     3541 cgagcccggc ctcttgtacg aggtcgtcta ccgggccggg cccgcggtgg tggtcggcct
     3601 cggcctcgcc gcgatccgcg attcgctgtc gtacttccgc tacgccgcgg cgccggccaa
     3661 cccgctcgcc ggcaccctcg accatgtgat cgcgttcggc atctcgcagt ccgggcgggt
     3721 gatcaaccag atgctgctcg acggcctgca tgtcgacgag cgcggccgcg gcgccttcga
     3781 tgccgccctc gtccacgtcg ccggcgccgg caagggcagc ttcaaccacc gcttcgccca
     3841 gaccacccgc cacccgagcc cgcaccagga ccaccagtac cccgccgatt tcttcccgtt
     3901 cgccaccgtc gcctccgggg acccggtgac cggcgagatc ggcgacctgc tggcaggtgc
     3961 gcgcggctcc ggggtggtgc ccaagatctt cttcaccaac acctcgggcg agtattggac
     4021 gcgcgcggca tcgctcctgc acaccgacgt gaccggcagc cgcgacctcg gcctcgccga
     4081 gtcggcgcgg ctctacgtct tcgcctccgg ccagcacggc agctggctgc tcgacaaccg
     4141 gctgtgggtc gagaactgca tcaacccgct cgactaccgg ccggcgatgc gggcgctctt
     4201 gctgcggctc gacgcctggg cgagcgaggg ccgcgcgccg ccggcaagcg tctatccgcg
     4261 catcaccgag ggcaccctcg gcacggtcgc ggactatcgc gcgaagttcc cggccatccc
     4321 ggggctgcgc ctgccgctcg tcaacctgcg cccgccgcgg ctcgagctcg ggccgcgctg
     4381 gagcgcgcag cggatcatcg accgggtgcc gcccggcttc ggcccggcct atgagacccg
     4441 ggtgccgatg ccggacggcg acggcatcga cctcggcggc attcgcctgc ccgaggtggc
     4501 ggcgccactc ggcaccttca ccggctggaa cctgcgccgc gaagggttcg gcgcgaccga
     4561 catgatcggg cgctggttcg gctcgttcat cccctttgcc gccgacgagg cggcgcgcgc
     4621 gcaagccgga gaccctcggc cgtcgcgcgc cgcgcgctac caatcgcgcg cggactatgt
     4681 ggccggggtc ggcgcggcgg ccgaggcgct ggcgggcgag ggcttcctgc gcgccgccga
     4741 ggtgccgcag atcgcagccc gcctcggcgc attctacgac cggttgcacg ctcgcgcgcc
     4801 tcggcccggc gcctgcgcct acatcgtgcc ccggtcctga gcgccgcgcc gggcttactc
     4861 ggtatcgtag aaggttatcc tgcggtaggt cacccgcacc gggcgccagc gctcctcgcc
     4921 gaactcgcgc agccaatgct cggtcgtgcg ccgaacccag ttgccgtgct cggagtgcgg
     4981 gtcgtagacc agccgccccc tgacctgcga gctcccgtcg tcatccaggc gggcacattc
     5041 gaccatgttg ccggaggagt catagcgatg caccagccgc tcgacgacgg cgccgtcctc
     5101 gcgcacctcg ccatgggccg cggctgccga cgcgccgcta tcgtaggcga tccgccagcg
     5161 ggtctcgcgg acgctgccgt cccggtcccg gacctcgacc accgagatca ggcggccggc
     5221 ttcgtcatat tggcgcttga gccctgcgca caccgcgcct tcgggcgaca ggccggtcat
     5281 ctcgaccagc cggccgcgcg cgtcgtagct gtacaggtaa cgcaggatgg cgcgacccgc
     5341 ggggtcgtag tggatgcgtt cgaccgctcg gccatcggcg tcgtagccga acaccgcccg
     5401 gtccaccagc gcgccggtct cgtcgtagcg gtcgcgctcg atcagcgcgc cggtggcgtc
     5461 gaagctttcg ctgcgcagct tgatcggcgg ctgctcgacc gcccggccga agctgtcctc
     5521 gatcaccgcc tcgaaggtgg tcaccgtgtg caccgggccg tacaggcgct cgacgccgcc
     5581 gcgcggggtg aacgcgaaca gcgtcagcca ggtctccgga accgtcgccg gcgtggtcgt
     5641 ttcggacatt gccgttcggc gaccggcgcc accgccaccc tccgggcccg gtcaccgaac
     5701 cgggccgcga gaggagccgc gaccgtcacc ctttcccctt ggaatcgtca gactggggcc
     5761 gaactattag caattcgtta acgaacgccg cggacagtgg ccgccatggc caccc
//
LOCUS       DASWHU010000200         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_98701, whole genome shotgun sequence.
ACCESSION   DASWHU010000200 DASWHU010000000
VERSION     DASWHU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98701"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VGB88_07200"
     CDS             complement(<1..870)
                     /locus_tag="VGB88_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9647269.1"
                     /translation="MDLSLSDDQRLLADSAARFVANEYGLDRRRALVADEPGFSRDHW
                     RTFAELGWLALPFAEADGGLGAGAAEVMVVMEQFGRGLVVEPYLAAVLLAGQAIAHAG
                     TAAERRALLAELIAGRAHYAFAHGEAGARYAAGHVETRAHADGGGWLLSGHKAVVYNA
                     PAADAFVVSARSAGDARDAHGVSLFVVAKDAAGLGVRPYRTIDGLRAGEVVLDGVRVG
                     ADALLGEAGAALPAIEEVVDRATAAVCAEAVGIMDTLREMTLDYLKTRQQFGRPIGAF
                     QALQHRAVDMLIAC"
     gene            complement(1032..2183)
                     /locus_tag="VGB88_07205"
     CDS             complement(1032..2183)
                     /locus_tag="VGB88_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9647270.1"
                     /translation="MRGFIAASLPGDVKAKVEGMLELGRDDYDRWHKILNAKGWVAPH
                     WPVEHGGTGWSPVRRYIFDEECALAGAPRVMPFGVNMVGPVIYTFGTEAQKAHYLPRI
                     LSNEDWWCQGYSEPGAGSDLAALRTRARRDGDHYLVSGQKTWTTTAHWANMIFCLVRT
                     EATAKPQEGISFLLIDMASPGVSVRPIITMDGGHEVNDVFLDDVRVPAANLVGEENQG
                     WTYAKFLLSHERSGIADVGRSKKQLQRLKRIAGAEMKDGRPLIEDARFRAHVAEAEIE
                     LLALEATELRALSEERAGRPPGPEANLMKIRGTEIQQRLCELMMEAVGVYAHPYVPEA
                     LAEGWNEAPVGPDYAAPLAARFFNWRKASIYGGTNEVQKNIIAKMVLGL"
BASE COUNT          299 a          798 c          775 g          312 t
ORIGIN      
        1 gcaggcgatc agcatgtcga ccgcgcggtg ctggagcgcc tggaaggcgc cgatcgggcg
       61 gccgaactgc tggcgggtct tgaggtagtc gagggtcatc tcgcgcaagg tgtccatgat
      121 gccgaccgct tccgcgcaca ctgcggcggt ggcgcggtcg acgacctcct cgatcgcggg
      181 cagcgccgcg ccggcctcgc cgagcaacgc atccgcgccg acgcgcacgc cgtcgagcac
      241 aacctcgccc gcgcgcaagc cgtcgatggt gcggtacggg cgcacgccaa gacccgccgc
      301 gtccttcgcc accacgaaca gcgacacgcc gtgcgcatcg cgcgcatcgc cggcgctgcg
      361 cgcggagacc acgaacgcat ccgccgcggg cgcgttgtag accaccgcct tgtggccaga
      421 cagcagccag ccgccgccgt cggcgtgcgc ccgcgtctcg acgtggccgg cggcgtaacg
      481 cgcgcccgcc tcgccatggg cgaaggcgta gtgggcgcgg cccgcgatca gctcggcgag
      541 cagcgcgcgg cgctcggccg ccgtgcccgc atgcgcgatc gcctggccgg cgagcaggac
      601 ggcggcgaga tagggctcga ccaccaggcc gcggccgaac tgctccatca ccaccatcac
      661 ctcggccgcg ccggcgccga ggccgccgtc ggcctccgcg aacggcagcg ccagccagcc
      721 gagctcggcg aaggtgcgcc agtggtcgcg gctgaagccg ggctcgtccg cgacgagtgc
      781 gcgccggcgg tcgaggccgt actcgttcgc gacgaaacgc gcggccgagt ccgcgagcag
      841 gcgctggtcg tcgctcaggc tgaggtccac cggcgtactc cgcggctcgc acgcattcga
      901 agccggctga atcgcgccac cccccacctg cctccccccg caagcggggg gaggtttgac
      961 gttgaaagcc ctgccccgcc ggcgggggag ggtttgggtg ggggtgcggc cgggtccggg
     1021 cggaacatgc gctagagacc cagcaccatc ttggcgatga tgttcttctg gacctcgttc
     1081 gtgccgccgt agatcgacgc cttgcgccag ttgaagaagc gcgccgcgag cggcgccgcg
     1141 tagtccggcc cgaccggcgc ctcgttccag ccctcggcca gcgcctcggg aacgtaggga
     1201 tgggcgtaga cgccgaccgc ctccatcatc agctcgcaca ggcgctgctg gatctcggtg
     1261 ccgcgaatct tcatcaggtt cgcctcgggc ccgggcggcc ggccggcgcg ctcctctgac
     1321 agcgcgcgca gctcggtggc ctcgagcgcg agcagctcga tctccgcctc ggcgacgtgc
     1381 gcgcggaagc gcgcgtcctc gatcagcggc cggccgtcct tcatttccgc gccggcgatc
     1441 cgcttcaggc gctggagctg cttcttcgag cggccgacgt cggcgatccc ggagcgctcg
     1501 tggctgagca ggaacttggc gtaggtccag ccttggttct cctcgccgac caggttcgcc
     1561 gccggcacgc gcacgtcgtc gaggaacacg tcgttgacct cgtggccgcc gtccatggtg
     1621 atgatcgggc gcacgctcac cccgggcgag gccatgtcga tgagcaggaa cgagattcct
     1681 tcctgcggct tggcggtggc ctcggtgcgc accaggcaga agatcatgtt cgcccagtgg
     1741 gcggtggtgg tccaggtctt ctggccgctg accaggtaat ggtcgccgtc gcgtcgcgcg
     1801 cgggtgcgca acgcggcgag gtcggagccc gcgcccggct ccgagtagcc ctggcaccac
     1861 cagtcctcgt tcgagagaat ccgcggcaga taatgcgcct tctgcgcctc ggtgccgaag
     1921 gtgtagatca ccgggccgac catgttgacg ccgaacggca tcacccgcgg cgcgccggcg
     1981 agcgcgcact cctcgtcgaa gatgtagcgc cggaccgggc tccagccggt gccgccgtgc
     2041 tccaccggcc agtgcggcgc cacccagccc ttggcgttga ggatcttgtg ccagcggtcg
     2101 tagtcgtcgc gcccgagctc gagcatgccc tcgaccttgg ccttgacgtc gccggggagg
     2161 ctcgcggcga taaacccgcg cacc
//
LOCUS       DASWHU010000201         8164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2695074, whole genome shotgun sequence.
ACCESSION   DASWHU010000201 DASWHU010000000
VERSION     DASWHU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695074"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGB88_07210"
     CDS             <1..376
                     /locus_tag="VGB88_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEX9647271.1"
                     /translation="PLNMAARWFGPHLAYTMAKYGMSLCVLGMAAEFRADGIAVNALW
                     PRTTIATSAVRNLLGGEAMVRRSRRPEIVADAAHAILTKPSREFTGHFCVDDEVLAAA
                     GVTDLAAYAVDPTADLAHDFFI"
     gene            complement(402..1514)
                     /locus_tag="VGB88_07215"
     CDS             complement(402..1514)
                     /locus_tag="VGB88_07215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9647272.1"
                     /translation="MPGWRTEQDGDLVIYRLLTLARSAVQHIGTQSNIAEQIGRNSDL
                     LVHLSDQGFGGLIPQAVVEPHPGDKLFIGRLEHQRQRFLVYCRAREFEAATQVALVDN
                     QRDLASGQVIYFAGDGAAAGEWAMDVAERIDRGELHEGTGAAEGEHARDHAAKEREPA
                     SAHWEPAGPGSDRNRHRYKRSSVFCAGKIVFEDETIDCDVLNLSASGAQIRVLGEAEP
                     PERFVLRIDGFGKFDCEVVRRAGEKYGVAFVDNPETVEKIVEDIINNPDKTNEIRAHP
                     RRLVLLSGAVYVENRPVECRVLDVSAGGARIRVDHRFDHDSRLALMIYRFGEFPVEVA
                     WEKDIDLGIMFIDDPSEIERIIGHILPKRGNQARRR"
     gene            complement(1684..2133)
                     /locus_tag="VGB88_07220"
     CDS             complement(1684..2133)
                     /locus_tag="VGB88_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647273.1"
                     /translation="MSRVDQDLAIDVWQFVRLMVELEARARARRAGAAYRAWREAWQE
                     LDRRLAELGTSDPAAYAELMMGQQVMLETPSPAELADAISALDAVIAQLAESIARGKV
                     GARRLADLRFERDELAALKTRLARRPAGKAGQRPSRARRSAGRGGGE"
     gene            2222..3037
                     /locus_tag="VGB88_07225"
     CDS             2222..3037
                     /locus_tag="VGB88_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9647274.1"
                     /translation="MADLPTLRDASLSLDGRVATLTMSRDEVRNALTGTSLADDIVTA
                     VTWASGCEEVSVLVITGAGAAFSSGGNIKDMRARAAGFSGPIPDIERAYRTGIQRLPL
                     ALARAELPLIAAVNGPAVGAGCDLACMCDLRVGSTAAAFAESFVNLGIIPGDGGGWFL
                     QRTIGYARAAEMTLTGRMIDARTALAYGLLNELVEPDRLMARAGELAAEIAAKPPQAL
                     RYAKRLLKIAQRTELPDFLDLCAVLQGVCHNTDDHLEAISAFLDKRTPHYQGR"
     gene            complement(3052..4239)
                     /locus_tag="VGB88_07230"
     CDS             complement(3052..4239)
                     /locus_tag="VGB88_07230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9647275.1"
                     /translation="MAGILDGIRVLDFGRYIAGPYCATLLAELGAEVIRIEKVGGSED
                     RTYAPVEPGKTGALFLQMARNKRGLTLKPTTPAGREIVRRLVARADVVVANLPPPALA
                     QMGIDYASLRAVKPDIILATTSAFGRGGPMSDRVGYDGIGQAMNGAMYLSGWPEAPMR
                     AYLPYVDFGTALYSAFGTLAALMERARSGQGQVVETSLLGTALSFNNAALIEQAVIKP
                     DRVGTGNRAQLSAPTDAFQTADGWLLVQVVGDQLFRRWAKLMGEDDPWLTDPRFADDR
                     GRGDHRDQVCERMAAWCGERTTEQCLAELEAARIPAGPVLSPQQALDHPHIRAMRLLL
                     PLDFPGLGTPAPVARVPLELSATPGAIRHRAPRLGEHSDAILAELGYGEAEIAALRAE
                     GTV"
     gene            complement(4251..6407)
                     /locus_tag="VGB88_07235"
     CDS             complement(4251..6407)
                     /locus_tag="VGB88_07235"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9647276.1"
                     /translation="MALFVSGSGPAIAAGAADLDTAQLETLVDTLEDDAKRRQFVDQL
                     QALIAARRAIEREAAVEETLGARVLSGLSRQIERIGVQLTQTAAVLAAVPETVTRIAA
                     QARQPSVRALWLEIAGKLALVLALALVACALTGRLLARVRAGLTAQGHQGPISLTLLF
                     LGRAVLAFVPLIAFAAIALVVLPLTEPRPATRILALGVINAAVLVQATLIVARLVLQN
                     GGERGGLLELSAETAAYLVIWTRRLAMIGIFGYFAAETAVVLGLAPGAHAMLLKALGL
                     VVAIMLVVLVLQNRHAVAAALGGEPRGDDAGRSDHRSRLAQLWHVPVLLYIAVGYGAW
                     AVQAGNGFMFLARATVASAALVVIAILVLKAIRLVFDRGLRVSAETARRFPGLETRAN
                     AYFGIVRAVLAALTYLVAAMLLIEVWIGGSIAWLSSDAGQVLVARAVTIAFVLAAATA
                     VWEVVTAVVERVLSTTDAAGPGSRGTRLRTLLPLIRNAVRAVLGVIVVLTVLSELGID
                     IAPLLAGAGIAGLAIGFGAQSLVKDVITGIFILLEDSIAVGDVVEVGGHAGVVEAMSV
                     RTVRLRDLSGSVHVVPFGEVASVLNMTKDFAFALMDVGVAYKEDVDQVIEVLKRLADE
                     MRADSDFAPMILEPLEVLGLESFGDSAVVIRVRLKTRPIKQWAVKREYQRRMKRRFDE
                     LGIEIPFPHRTIYYGDAGAPKLGAAASPAVDAPGNK"
     gene            6672..>8164
                     /locus_tag="VGB88_07240"
     CDS             6672..>8164
                     /locus_tag="VGB88_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9647277.1"
                     /translation="MALAGPSLSADAADIAYRVAFTGLEALPEIAETLEGVSRLATET
                     GRPPATVRQLRRRIADDVPRLIEVLRSYGHYAATVETSLDSAQSPAVVTVAVAPGPPY
                     LLSAFRVAARGGAGGPVVRVPLAELGVETGMPARAAAIAGADAGLVRALTESGYPRAR
                     VVDREVLVDHDLKSVAVTVAVDLGPAARFGPTSFVGIESVDSGFVARRVQWQTGDRYD
                     SRKVAATRDALRATQLFSSVRIDVGEPAAGDDAAPMTVTLVERAQRSVGAGAGYSTSE
                     GLRGKLFWEHRNLFGEGERLRATALAAEIVTSLSASFSKPDFLDPGQELLLSGAIEQQ
                     RTDAFETERVTASGRLRRPLYRRIQVSGGLALAREFEDDNTVQRRFTLVSLPVTLEVD
                     TSDEPLDPSRGARGRFQVSPTLQALGSDVSFVTLDLSESRYLSLAADDSLVLAGRYRV
                     GSVVGVTAGELPASARFFGGGGGSVRGFAFQSIGPRDGFNEPFGGSS"
BASE COUNT         1185 a         3021 c         2820 g         1138 t
ORIGIN      
        1 gccgctcaac atggcggcgc gctggttcgg cccgcacctc gcctacacca tggcgaagta
       61 cggcatgagc ctgtgcgtgc tggggatggc ggccgagttc cgcgccgacg gcatcgcggt
      121 caacgcgctg tggccgcgca ccacgatcgc gacatcggca gtccgcaacc tgctgggcgg
      181 ggaggcgatg gtgcgccgct cgcgccggcc cgagatcgtg gccgatgccg cgcacgcgat
      241 cctcaccaag ccgagccgcg agttcaccgg ccatttctgc gtcgacgacg aggtgctggc
      301 ggcggccggg gtgaccgacc tcgcggccta tgcggtcgac ccgacggcgg acctcgcaca
      361 cgacttcttc atctgagtcg ctgggcggcc gcccgccgcg gtcagcggcg gcgagcctgg
      421 ttgccgcgct tgggcaggat gtggccgatg atccgctcga tctcggacgg gtcgtcgatg
      481 aacatgatgc cgaggtcgat gtccttctcc cacgccacct cgaccgggaa ctcgccgaat
      541 cggtagatca tcagcgccag gcggctgtcg tggtcgaacc ggtggtcgac gcggatgcgc
      601 gcgccgccgg cagagacgtc gagcacccgg cactcgaccg gccggttctc cacatagacc
      661 gcgcccgaca gcagcaccag ccggcgcgga tgcgcacgaa tctcgttggt cttgtcgggg
      721 ttgttgatga tgtcctcgac gatcttctcg accgtttccg ggttgtcgac gaacgcgacc
      781 ccgtacttct cgccggcgcg gcgcaccacc tcgcagtcga acttgccgaa gccgtcgatg
      841 cgcaggacga atcgctcggg cggctcggct tcgccgagga cgcggatctg cgcgccgctc
      901 gcggacaggt tgagcacgtc gcagtcgatt gtttcgtctt cgaaaacaat ctttcccgcg
      961 cagaacaccg agctgcgctt gtagcgatgg cggttgcggt ccgagccggg tccggccggc
     1021 tcccagtgcg ccgaggcggg ctcgcgctcc ttggcggcgt ggtcccgggc gtgctcccct
     1081 tcggccgcgc cagtgccctc atgcagctcg ccgcgatcga tgcgctcggc gacgtccatc
     1141 gcccattcgc cggcggcggc cccgtcgccg gcgaaataaa tcacctggcc gctggcgagg
     1201 tcgcgctggt tgtcgaccag cgctacttgc gtcgcggcct cgaactcgcg cgcccggcaa
     1261 tagaccagga acctttggcg ctggtgctcg agccggccga tgaacagctt gtcgccgggg
     1321 tgcggctcga ccaccgcctg gggaatcagg ccgccgaaac cctggtcgga caagtgcacc
     1381 aaaaggtccg aattgcgccc gatctgctct gcgatattgg actgcgtgcc gatatgctgg
     1441 accgcgctac gggccagcgt cagcagacga tagatgacga gatcgccgtc ctgctcggta
     1501 cgccatccgg gcaccccctt ttggcccaga tgatcttgac gagcggaccc cttgttcgcc
     1561 atcgcccaag cgtcccctgg cgcgcgggcc gcattccccc gaaagcgcgc acgccggctc
     1621 atgttaaccg cgaattatgt catatatagt gctttttcgg aaccggtccc gcctggcgcg
     1681 gttttattcc ccgccgccgc ggccggccga acggcgggcg cgggacgggc gttggccggc
     1741 cttgccggcc gggcggcggg caaggcgcgt cttgagggcg gcaagctcgt cgcgctcgaa
     1801 tcggaggtcg gccaggcggc gggcgccgac cttgccgcgc gcgatcgatt cggccagctg
     1861 cgcgatcacg gcatcgaggg cggagatcgc gtccgcgagc tcggcggggc tcggcgtctc
     1921 cagcatcact tgctggccca tcatcagctc ggcgtaggcg gcggggtcgc tggtgccgag
     1981 ctcggcgagg cggcggtcga gctcctgcca cgcctcccgc caggcgcggt aggccgcccc
     2041 ggcacggcgc gcgcgcgccc gcgcctcgag ctcgaccatg agccggacga actgccagac
     2101 atcgatggcg agatcctggt cgacgcgact catcccggca ccctgcgcgg cccttatact
     2161 ggccgcggcg ggcgcaggcg agccccgcac gcgaccacgt ccaaacctgg agcccagacc
     2221 catggccgat ctgccgacgc tccgcgacgc cagcctcagt ctcgacggcc gcgtcgcgac
     2281 cctgaccatg agccgcgacg aggtgcgcaa cgcgctcacc ggcacctcgc tcgcagacga
     2341 catcgtcacc gcggtgacct gggcgagcgg ctgcgaggag gtgtcggtcc tggtcatcac
     2401 cggcgccgga gccgcgttca gctcgggcgg caacatcaag gacatgcgcg cgcgcgccgc
     2461 cggcttctcc ggcccgatcc cagacatcga gcgggcctac cggaccggga tccagcgcct
     2521 gccgctcgcg ctcgcgcgcg ccgagctgcc gctgatcgcg gcggtcaacg gcccggcggt
     2581 cggcgccggc tgcgacctcg cctgcatgtg cgacctcagg gtcggctcga ccgcggccgc
     2641 gttcgccgag agctttgtca atctcggcat catcccgggc gacggcggcg gctggttcct
     2701 gcaacggacg atcggctatg cgcgcgccgc cgagatgacc ctgaccgggc ggatgatcga
     2761 tgcacggacc gcgctcgcct acggcctgct caacgagctg gtcgagcccg accggctgat
     2821 ggcgcgcgcc ggcgagctgg ccgcggagat cgccgccaag ccgccccagg cgctgcgcta
     2881 cgccaagcgc ctgctaaaga ttgcccagcg caccgagctg ccggacttcc tcgacctgtg
     2941 cgccgtgctc cagggcgtct gccacaacac cgacgaccac ctggaggcga tcagcgcctt
     3001 cctcgacaag cgcacgcccc attaccaggg gcgctgactt gggactgggc gtcacaccgt
     3061 gccctcggcc ctgagcgcgg cgatctcggc ctcgccgtag ccaagctcgg ccaggatcgc
     3121 gtcgctgtgc tcgccgagac gcggcgcgcg gtggcggatc gcgcccggtg tcgccgacag
     3181 ctcgagcggc acccgcgcca ccggcgcggg cgtgccgagc ccgggaaagt cgagcggcag
     3241 caacagccgc atcgcgcgga tgtgcggatg gtcgagcgcc tgctgcggcg acagcaccgg
     3301 ccccgccggg atccgcgccg cctcgagctc ggccaggcac tgctcggtgg tgcgctcgcc
     3361 gcaccaggcg gccatgcgtt cgcacacctg gtcgcggtga tcgccgcggc cgcggtcgtc
     3421 ggcgaagcgc gggtcggtga gccaggggtc gtcctcgccc atcagcttgg cccagcggcg
     3481 gaacagctgg tcgccgacca cctgcaccag cagccagccg tcggcggtct ggaacgcgtc
     3541 ggtcggcgcc gacagctggg cgcggttgcc ggtgccgaca cggtcgggct tgatcaccgc
     3601 ctgctcgatc agggcggcgt tgttgaacga caacgcggtg ccgagcaacg aggtctcgac
     3661 cacttggccc tgcccggacc gcgcgcgctc catcagcgcc gccagggtgc cgaaggcgga
     3721 atagagcgcg gtgccgaagt cgacataggg cagataggcg cgcatcggcg cctcgggcca
     3781 gccgctcaga tacatcgccc cgttcatcgc ctggccgatg ccgtcgtagc cgacccggtc
     3841 gctcatcggc ccgccgcggc cgaacgccga ggtggtcgcc aggatgatgt cgggcttgac
     3901 cgcgcgcagg ctggcatagt cgatgcccat ctgggcgagc gccggcggcg gcaggttggc
     3961 gaccaccacg tcggccctcg ccaccagccg gcgcacgatc tcgcgccccg cgggcgtggt
     4021 cggcttcagg gtcaggccgc gcttgttgcg cgccatctgc aggaacagag cgccggtctt
     4081 gcccggttcc accggcgcgt aggtccggtc ctcgctgccc ccgaccttct cgatccggat
     4141 cacctcggcg ccgagctcgg cgagcaaggt ggcgcaatag ggcccggcga tgtagcggcc
     4201 gaaatcgagc acccggatgc cgtcgagaat ccccgccatc gccgccgccc tcacttgttg
     4261 cctggagcgt ccaccgccgg tgacgccgcc gcgccgagct tgggcgcgcc cgcgtcgccg
     4321 taatagatgg tgcggtgcgg gaacgggatc tcgatcccca gctcgtcgaa ccgccgcttc
     4381 atgcgccgct ggtactcgcg cttgaccgcc cactgcttga tcggccgggt cttgagccgg
     4441 acgcggatca cgaccgccga gtcgccgaac gattccagcc ccagcacctc cagcggctcg
     4501 aggatcatgg gcgcgaagtc ggaatccgcg cgcatctcgt cggcgagccg cttgagcacc
     4561 tcgatcacct ggtcgacgtc ctccttgtag gcgacgccca cgtccatcag ggcgaaggcg
     4621 aagtccttgg tcatgttcag cacgctcgcg acctcgccga acggcaccac gtggacactg
     4681 ccgctgaggt cgcgcaggcg caccgtgcgc acgctcatcg cctcgaccac gccggcgtgg
     4741 ccgccgacct cgaccacgtc gccgaccgcg atcgagtcct cgagcaggat gaagatgccg
     4801 gtgatcacgt ccttgaccag gctctgggcg ccgaagccga tcgccaggcc ggcaatgccg
     4861 gcgccggcga gcagcggcgc aatgtcgatg ccgagctcgg acagcaccgt cagcacgacg
     4921 atgacgccga gcaccgcccg caccgcgttg cggatcagcg gcagcaaggt cctgagccgc
     4981 gtgccccggc tgcccgggcc ggcggcgtcg gtggtcgaca gcacccgctc gaccaccgcg
     5041 gtcaccacct cccacactgc cgtcgccgcc gccagcacga aggcgatcgt caccgcccgc
     5101 gccaccagca cctggccggc gtcgctcgac agccaggcga tcgagccgcc gatccagacc
     5161 tcgatcagca gcatcgccgc gaccagatag gtgagcgcgg ccagcaccgc gcggacgatg
     5221 ccgaaatagg cgttggcccg ggtctcgagc ccgggaaacc ggcgcgcggt ctctgccgac
     5281 accctgagcc cgcggtcgaa caccagccgg atcgccttga gcaccaggat cgcgatcacc
     5341 accagcgcgg cgctggcgac ggtggcgcgg gcgaggaaca tgaaaccgtt gccggcctgc
     5401 accgcccagg cgccgtagcc gacggcgata tagagcagca ccgggacgtg ccacagctgc
     5461 gccaaccgcg agcgatgatc cgaacgcccc gcgtcgtccc cccgcggctc gccgccgagc
     5521 gccgccgcca ccgcgtggcg attctgcagc accagcacca ccagcatgat cgccaccacc
     5581 aggccgagcg ccttgagcag catcgcatgg gcgcccggcg cgagaccgag cacgaccgcc
     5641 gtctcggcgg cgaagtagcc gaaaatcccg atcatcgcca gccggcgcgt ccagatcacc
     5701 aggtaggcgg cggtctcggc gctgagctcg agcaggccgc cacgctcgcc gccgttctgc
     5761 agcaccagcc gcgccacgat cagcgtcgcc tggaccagga ccgcggcatt gatcacgccg
     5821 agggcgagga tgcgcgtcgc cggccgcggc tcggtcagcg gcagcaccac cagcgcaatg
     5881 gcggcgaacg cgatcagcgg cacgaacgcc agcaccgccc gcccgaggaa cagcagcgtg
     5941 agcgagatcg ggccctggtg gccttgcgcc gtgaggcccg cgcgcacccg cgccagcagg
     6001 cgcccggtga gggcgcacgc gaccagggcc agcgccagca ccaaagcgag cttgccggcg
     6061 atctcgagcc acagcgcgcg caccgacggc tgtcgcgcct gcgccgcgat ccgggtgacc
     6121 gtctccggca cggcggcgag caccgcggcg gtctgggtga gctggacgcc gatccgctcg
     6181 atctgtcggg acaatcccga cagcacccgg gcgccaagcg tctcctcgac tgcggcctcg
     6241 cgctcgatcg cgcgccgcgc ggcgatcagg gcttggagct ggtcgacgaa ctggcgccgc
     6301 ttggcgtcgt cctcgagcgt gtcgaccaag gtctcgagct gcgcggtgtc gagatcggcg
     6361 gcgccggcag cgatcgccgg cccggaccca gacacaaaca atgccaacgc gagcaacgca
     6421 ccggcaagcc tcgcgccgcg cggccgcgat cgcggcgcgc cgcaggaaac gcaattcatg
     6481 acccgtcaac ctagccagcg gcgcatccgc tgtcacatac tctgtgccgg cgaaggggcg
     6541 cgccggccgt tccgacgccg gtgccagcgc tttcgggccg accgtgttgg caaaaatgga
     6601 gcgccgtcgc actcgtgaag gcagaccgag caacgcttgc ccgagtcgca cgccgcgccg
     6661 ccgcgctgct gatggcgctc gccggaccga gcctgtccgc cgacgccgcc gacatcgcct
     6721 atcgggtcgc gttcaccggc ctcgaggcgc tgcccgagat tgcggagacg ctcgaaggcg
     6781 tctcgcgcct cgccaccgag accggccggc cgccggcgac ggtgcgccag ctcaggcgcc
     6841 gaatcgccga tgacgtgccc cgcctgatcg aggtcctgcg gtcgtacggc cattacgccg
     6901 cgacggtcga gacctcgctc gacagcgcgc agtcgccggc cgtggtgacg gttgcggtcg
     6961 cgcccgggcc accctatctc ctgtccgcct ttcgcgtcgc cgcccggggc ggtgccggcg
     7021 ggccggtggt acgcgtgccg ctcgccgagc tgggcgtgga aaccgggatg cccgcgcgcg
     7081 ccgcggcgat tgccggcgcc gatgccgggc tggtgcgcgc gctgaccgag agcggctacc
     7141 cgcgtgcgcg cgtcgtcgat cgcgaggtcc tggtggatca cgatctcaaa tcggtcgcag
     7201 tgactgtcgc cgtcgacctc ggtccggcgg cccggttcgg gccgaccagc ttcgtcggca
     7261 tcgagtcggt cgattccggc ttcgttgcgc gcagggtcca gtggcagacc ggcgaccgct
     7321 acgattcccg caaggtcgcc gccacgcgcg atgcgctgcg cgcgacccag ctgttcagct
     7381 cggtacgcat cgacgtcggc gagcccgccg cgggcgacga cgcggcaccg atgacggtca
     7441 cattggttga gcgcgcacag cgcagcgtcg gcgccggcgc cggctactcg accagcgagg
     7501 gcctgcgcgg caagctgttc tgggagcacc gcaacctgtt cggcgagggc gagcggctgc
     7561 gcgcgaccgc gctcgccgcc gagatcgtca ccagcctgtc ggccagcttc agcaagcccg
     7621 atttcctcga cccgggccag gaattgctgc tcagcggcgc catcgagcag cagcgcacgg
     7681 acgccttcga gaccgagcgg gtcaccgcct cgggccgctt gcgtcggccg ctgtaccggc
     7741 gcatccaggt cagcggcggg ctcgcgctgg cgcgcgagtt cgaggacgac aacaccgtcc
     7801 agcgccgctt caccctggtg tcgctgccgg tcaccctcga ggtcgatacc agcgacgagc
     7861 cgctcgatcc gagccggggc gcgcgcggcc ggttccaggt ctcgccgacc ttgcaggcgc
     7921 tcggcagcga cgtgagcttc gtcaccctgg acctctccga gtcgcgctac ctgtcgctgg
     7981 ccgccgacga ctcgctggtg ctcgccggac gctaccgggt gggcagcgtg gtcggcgtca
     8041 ccgccggcga gctgccggcg agcgcccggt tcttcggcgg cggcggcggc tcggtgcgcg
     8101 gcttcgcgtt ccagtcgatc ggcccgcgcg acggtttcaa cgagccgttc ggcggcagct
     8161 cgat
//
LOCUS       DASWHU010000202        18022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1588940, whole genome shotgun sequence.
ACCESSION   DASWHU010000202 DASWHU010000000
VERSION     DASWHU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588940"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VGB88_07245"
     CDS             <1..374
                     /locus_tag="VGB88_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647278.1"
                     /translation="QVRAPAAAPAPQAAPVLAGRRASDHQPEPEPAVAVDPPAAAEAE
                     AEAAVRPRLFDWMKRAGRTSDDQPAPAAPAPAARPATRHEPAAMAPAMAAPAPERATV
                     GGGRRDDDLLDIPAFLRRQST"
     gene            564..1478
                     /gene="lpxC"
                     /locus_tag="VGB88_07250"
     CDS             564..1478
                     /gene="lpxC"
                     /locus_tag="VGB88_07250"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX9647279.1"
                     /translation="MQHTATTVDQTTIRQPIDCTGVGLHSGAVARMMLCPAERDTGIV
                     FVRADLGGSEIPARYDHVVDTRLGTTLAGPDGALVHTVEHLMAALWGCGIDNLVVELD
                     GPEVPVMDGSAEPFVFLVECAGIRTLGAARQAVRVLRPVTVTDGDTRIALVPDDRTSV
                     RFEIAFDNPVIGNQVLDLACDDCAFKTDLARARTFGLESDVAAMHANGRGLGGSLGNA
                     VVVGKDRVLNEGGLRFPDEFVRHKILDCLGDLYLAGARIVGRIEAVRAGHRLNNALLR
                     ALFAEPANWCLEAAAPRTWTDDAVAALA"
     gene            1538..2518
                     /locus_tag="VGB88_07255"
     CDS             1538..2518
                     /locus_tag="VGB88_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX9647280.1"
                     /translation="MSDAVAPAFELAGRRVWVAGHGGMVGAALCRRLARERCELLSVG
                     RDRVDLRRQAEVEDWMAAARPAAVFVAAATVGGILANDSRPAEFLYDNLAIETNVIEA
                     ARRTGVAKLLILGSTCIYPRLAPQPMREDSLLTGPLEPTNQWYALAKIAGIKLCDAYR
                     RQYGCDFISAQPTNLYGPHDNFDLASSHVLPALMRKMHAAKLAGADTVTLWGSGTPRR
                     EFLYVDDLADALVFLMRHYSTEGPINVGTGEELSIRELAERIAAVVGFAGRFAYDTTK
                     PDGTPRKLADVGKLAALGWRARTSLDDGLGLTYRWFLDHVADRLAAAAAG"
     gene            2526..3215
                     /locus_tag="VGB88_07260"
     CDS             2526..3215
                     /locus_tag="VGB88_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX9647281.1"
                     /translation="MPDPASHLVDGVGLWRELLRAPPAGPPRPALFVDRDGVVIEDAG
                     FVHRPDQVSLIAGAAAAFACCNRAGVPVVMVSNQSGIGRGLYGWAEFAAVQARMAALL
                     AAAGAALDMVLACAYHADGVGALKVAGHDWRKPRPGMLCAAAEAWPIDLARSWIVGDR
                     SRDLAAGRAAGLAGGVLVATGHGSESSERDAAQLLAGERFEVRGTRSIAAIDAVLARL
                     GAGLEPARRAR"
     gene            3212..4189
                     /locus_tag="VGB88_07265"
     CDS             3212..4189
                     /locus_tag="VGB88_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9647282.1"
                     /translation="MTVLVTGAAGFIGFHVASALLARGESVVGVDDLNPYYDVALKQA
                     RLAQLEGRAGFRFRRLDIADAAAMEQLARAEGPFRRIVHLAAQAGVRHSLTQPMAYGR
                     SNLVGQLVVLELARHTEGLRHLVFASSSSVYGGNAKLPFAVADRVDTPVSLYAATKRA
                     GELMAYSYCRLYGLPTTGLRFFTVYGPWGRPDMAAWLFTEAILAGRPIKVFNHGDMRR
                     DFTYVDDIVDGVVRVIDRPPPAGEAPPWHLYNIGNHRPEPLLRFIAVLEAALGRRAET
                     EMLPMQPGDIAETYADIDELSRDTGFRPTTTIEEGIPRFVAWYRDHYGV"
     gene            complement(4177..5247)
                     /gene="rfbB"
                     /locus_tag="VGB88_07270"
     CDS             complement(4177..5247)
                     /gene="rfbB"
                     /locus_tag="VGB88_07270"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9647283.1"
                     /translation="MKVLVTGGAGFIGSALVRHLLRDAGHEVVNLDKLTYAANPASLA
                     EVADHPNYRFVHADICDARAVAAAFDAHDPDAIVNLAAESHVDRSIDAPAGFLHTNVE
                     GTYVLLEAARRHWQGLDDARRARFRFHQVSTDEVYGALGPEGRFTEATPYAPSSPYSA
                     SKAAADHLARAWQRTYGLPVVVSNCTNNYGPRQFPEKLIPLAILNALEGRALPVYGDG
                     GQVRDWLHVEDHARALAAVLTRARPGSTYNIGADCERRNIDVVRAICRLVDAADPGSP
                     HNPRERLITRVADRPGHDRRYAMDAGRARADLGWRPEIDFNDGLARTVRWYLDNEAWW
                     RPLRERVYGGERLGLGAAAHTP"
     gene            complement(5244..5873)
                     /gene="rfbC"
                     /locus_tag="VGB88_07275"
     CDS             complement(5244..5873)
                     /gene="rfbC"
                     /locus_tag="VGB88_07275"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX9647284.1"
                     /translation="MLPEPGQTAPEDFDGVDIAATAIPEVIRLTPKRFGDARGFFAET
                     YSRRALAEAGIDIDFVQDNLAYSRAAGTVRGLHFQAPAAQAKLVGVVTGAVLDVALDI
                     RGGSPTFGRHVAVTLTAAAGEQLLVPVGFAHGYCTLEPDTRVAYKVSDVYAPEHERGI
                     LWHDPALAIRWPVAPADAEVSERDRSWPRLDQIESPFAYAAGRAQGAAR"
     gene            6061..7053
                     /locus_tag="VGB88_07280"
     CDS             6061..7053
                     /locus_tag="VGB88_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9647285.1"
                     /translation="MSSARRPSARTWARLGVAALAALLLYLAFRGVDWAELGALLAGA
                     DPARVGLAVLIMTAGTMLRAQRWRLLVAARVEPGYPIMFWATMAGYVGNAYLPARAGD
                     LLRSLLLGRHLGIRKSFVLGTTVTERVLDTIVIVALAGAVVALLPEAPHWLASSSTGL
                     SVGAACALVALLAVPRLAGYIEGWVARLPMRREWRARLIEIWHEFFTGSRAIENHVTA
                     AAFAALSVLIWLMDTACMTTVAAALGLHMITPLLALLLVVALALSSVAPSTPGYVGVY
                     QFVAVSVLAPFAVGQSQALALVLLFQAVIYVVVTPWGLIGLWRLGGLGAARAGP"
     gene            7080..7661
                     /locus_tag="VGB88_07285"
     CDS             7080..7661
                     /locus_tag="VGB88_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9647286.1"
                     /translation="MTGALAEYLDAAAGLLRETAGAGLDRPVAAAVAAISTALAAGKP
                     LLVCGNGGSAADAMHIAGELVGRYRLERPGLKAIALGTDAAVTTAWANDYDFETVFAR
                     QVEALGEAGGVVWAISTSGESANVVRALEAARARAMTTVALTGAGGGRLAALADVLIA
                     VPSRDTPRVQEVHTCLYHFICEQVERRIAGAAG"
     gene            complement(7666..8547)
                     /gene="rfbD"
                     /locus_tag="VGB88_07290"
     CDS             complement(7666..8547)
                     /gene="rfbD"
                     /locus_tag="VGB88_07290"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9647287.1"
                     /translation="MPAATVVVFGGGGQLGRELARATAPDGFQVRALAHAEADVADPR
                     AVRAAIAGARAAITAAAYTDVDRAESEPAPAFAVNQAGAGAVAEACAAARIPLVHVST
                     DYVFDGTANRPYREDDARAPLGVYGASKAAGEDAVRAAGGTAAILRTAWVFSPFGRNF
                     VTTLLRLGAERDEVAVVCDQQGGPTAARDLAHAALTIAAGLAAGGKGTGTFHYCGAPP
                     VSRSAFAQEIFAGAASRGLPAPQRLRAIATADHPTPARRPANSVLDCARIAAVWGLAQ
                     PDWRQGLAACLDRLAGT"
     gene            complement(8570..9097)
                     /locus_tag="VGB88_07295"
     CDS             complement(8570..9097)
                     /locus_tag="VGB88_07295"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9647288.1"
                     /translation="MPIKDLIRTIPDYPKAGIMFRDITTLLADARGFRTAVNELVNRY
                     TGAGIDKVAAIEARGFILGAPIAHQLGVGFVPVRKQGKLPGNTIEHHYDLEYGTDTVE
                     VHTDAIAAGDRVLVVDDLIATGGTAEAAVNLIEKSGGRVVECCFIVDLPDVGGRARLE
                     KAGHKVFALCAFEGE"
     gene            9217..10479
                     /locus_tag="VGB88_07300"
     CDS             9217..10479
                     /locus_tag="VGB88_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX9647289.1"
                     /translation="MHIRTLAVIGLGYVGLPLAVALARSRKVVGFDLSGRRIAELTAG
                     RDRSGEVPNEALAAVEIAFTGEVEALNGLDAYLITVPTPVDGSKKPDLSAVERACATV
                     GAALAPGAVVVLESTVYPGTVEEVCAPILERASGLAAGRDFFLGYSPERINPGDAAHQ
                     LGAITKVVAGQTPAVTAVLAELYGAIGPVFVAADIRTAEAAKVIENAQRDINIAFVNE
                     LAVIFGRLGLDTGAVLDAAATKWNFLPFRPGLVGGHCVGVDPYYLTYVAERSGYHPDV
                     ILAGRRINDTMGQHVGREVARRVHALALPRRCLVLGITFKENVRDIRNSGSAGVAREL
                     AAFGLEVDVHDPLADADEVRAAYGFELVASPAGGYGAVVLTVGHRAYAGWPASEVRAL
                     LAGGGLVADVRGIWRGLDFGDDIQVWRL"
     gene            10574..11530
                     /locus_tag="VGB88_07305"
     CDS             10574..11530
                     /locus_tag="VGB88_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647290.1"
                     /translation="MKSGRNGFVSRACRLAGAALVCVGAAALVAYGPAAAQEEGEAVS
                     PDSLKHGKITFPSGSVYEGDLSDGRMHGTGKIVYADGGVYEGSFQDDKITGRGVFVYP
                     NGDRYEGSFVDGQRHGDGVFAFANGAKYEGSFEGGQMHGQGTLTFANGAVYTGAFVNG
                     RREGEGKLSFASGDVFTGTFKGGEMDGKGRLQYAEGHVYEGEFKGGKRHGQGTFRMSN
                     GDAYAGEYKDGERNGRGTYTQADGVKYVGDYADGKRQGEGSILYPNGDRFEGKFAADL
                     QQGPGKLVYQDGTQVEVTYEAGKPVGKAVWIYPDGHRIEGEF"
     gene            11657..12532
                     /locus_tag="VGB88_07310"
     CDS             11657..12532
                     /locus_tag="VGB88_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647291.1"
                     /translation="MAIESQSPGRRSGRPADPGGELRRQLDQAHRAMWALYKELDDKN
                     AALNRSNEELEQFATIAGHDLQEPLRKVIAFAALLREEHGQGLGAGGRDYLARMDGAC
                     ERMLVLIEDLLRLARVTSQARPFERCDLAAVAAEVAADLGAQVERERGEVVIGPLPVI
                     EADPVQLRQLLQNLVSNGLKFHHPDRPPRVTVSVRRLDGDRIEVQVADNGIGFKAKFA
                     ERIFQPFRRLHGQGRYPGTGMGLAIVRKIAQRHGGEVRVESTLGEGSRFVVALPCRHG
                     RQAAGESARAAGDGR"
     gene            12522..13871
                     /locus_tag="VGB88_07315"
     CDS             12522..13871
                     /locus_tag="VGB88_07315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9647292.1"
                     /translation="MAAEHPRRRSSDLPRLRILLVEDNPDDVVLFGELLGRIAPLSRF
                     DHVDSPDEARRRLHGAGYDLCFFDYELGDETGLDLLHTVRADGYDRPIILLTGQGDET
                     VAVKAMRAGASDYLSKHGLSVGVLGGAIRHAMQLYETEQRRQRFERALRLSEQRYREL
                     VNRLPLVVGELAETGEARFVNEAASQVTGYAADELVGRDWWRLLAPDLDAAELDLLRQ
                     RVLAEGLNAIEMPARHKDGRMLTMEWSTAVMRQAEGGGETIVCIGMDVTERARMRDEL
                     MWLAVRDELTGLSNRRGFLTLAEQQLKAAARDKHSMLAVFVDLDGLKSINDQLGHAEG
                     DRALSDMAEVMRRTFRGCDVIGRIGGDEFVALVTEGSPFRSDGIIQRLQTVLAQFNAD
                     ETRRYYLSASIGVARYDPDTPIAIDHLVNRADSEMYENKQRRRRRDVVRKATPVAAG"
     gene            13908..14837
                     /locus_tag="VGB88_07320"
     CDS             13908..14837
                     /locus_tag="VGB88_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647293.1"
                     /translation="MANLSRSKSLGRAVGAALGAVVAAAPGAAWACCHGGGGGGGASV
                     PPSMPPVMPPMIVPSVPAAPTAVQDAVRDALRPDTPRREPSIPEMRSADPRPSIPEMR
                     ATDPAEVERMEREEAAHDEARRRQTERDIERWEEWRDRGKEIKVVVGWANNVAGGAAI
                     AACATGVGCIPVLIYAGGKLIEVLLVSHAEASRGTGFVERLVNGSASSADYWRRVRVN
                     GFGTMASEAIGLAGDAVGLPGVHSGGIGSMTQDAMGESLNAGYESIRDGLHEQNERQR
                     QEIWQRQADRATMEAYGLNTAGGFPMPAGAPYR"
     gene            14834..15970
                     /locus_tag="VGB88_07325"
     CDS             14834..15970
                     /locus_tag="VGB88_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9647294.1"
                     /translation="MTGPRARVAGAGALLAALVFAALVPAAAQDAGTPAIDPAARAAL
                     SVLEDRYTPDSEAVVRALAARLRGLADRPGGGLSVAFTDPAPIEAAAPALRADPPLAV
                     DGFAITAYAADPARPTRHALHGLLSFTDRFQRRLSVTFGADYEVADDLVTVGAVSVAP
                     YFPPVPAVVMLFVPANRVAADFWTRHAGLDDLLDFVAENAVSFALPWAVPRVPEDHYA
                     MAFFYEQLPRAARVDLLVADAPEGLTGERRSTVVLREGGWQVAIARVHLAFAERATVY
                     FKALYWPSDDVSGPEGAVRIAEVFSNRIPDLAARPRPAVEAPEPAPAPAVAAPAAPPA
                     PEATPAAAADSVEQRLERLRQLYQRELISREEYDAKRQALLDQL"
     gene            15990..16664
                     /locus_tag="VGB88_07330"
     CDS             15990..16664
                     /locus_tag="VGB88_07330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9647295.1"
                     /translation="MTRSESETWRAYYAATGARAPRATLLIALDRFAAEGADAGKLHA
                     LDLGCGSGRDAVELLARGWSVLAIDAEPAAIEALRARPEITASARSRLETRVARFEAA
                     RWPRVDLVNASFALPLVPPAAFAGLWRRIVASLKPGGRFAGQLYGERDGWAGDPTITF
                     FSRSQVEALLAGLEVEHLLEEEDDSVTPRGAPKHWHIFHVVARKPHDRAKEGSTTDST
                     KDTAAG"
     gene            complement(17064..>18022)
                     /locus_tag="VGB88_07335"
     CDS             complement(17064..>18022)
                     /locus_tag="VGB88_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9647296.1"
                     /translation="KPSEFTSTSVLEFVKLFEQAGFPPGVVNVVTGFGAEVGQPLVEH
                     PLVRKVAFTGSERTGQKVNEMAAPGFKKVGLELGGKSPNIVFADANLDNAVKGAVAGI
                     FAATGQTCIAGSRLLVQEQIHNRFVDRLVDFAKGARMGNPMEMDTQVGPVTTPPQYQK
                     VLDYIGIASAEGAELRLGGRPAQRPECGEGWFVEPTIFTGVDNRMRIAQEEVFGPVLS
                     VIPFTDDDQAIAIGNDVAYGLAAGVWTQDITRAHRMAKELQAGTVWINTYRAISFTSP
                     FGGYKRSGMGRENGQDMIRDYLQTKSVWVDMNAEFPNPFAMR"
BASE COUNT         2408 a         6469 c         6617 g         2528 t
ORIGIN      
        1 cccaggtccg cgccccggcc gccgcgccgg cgccgcaggc ggcgccggtc ctcgccgggc
       61 gccgggcgag cgaccaccag cccgagcccg agccggcggt cgctgtcgac ccgcccgccg
      121 cggccgaagc cgaagccgag gccgcggtgc gcccgcgcct gttcgactgg atgaagcggg
      181 cggggcggac gtccgacgac cagcccgcgc cggccgcgcc ggcgcccgcc gcccgcccgg
      241 cgacaaggca cgagccggcc gcgatggcgc cggcgatggc tgcgccggcg cccgaacggg
      301 ccaccgtcgg cggcggccgt cgcgacgacg acctgctcga cattccggcc ttcctgcgcc
      361 gccaatcgac ctgagcggac aggggtaaca ttctgtaaca aatagtgatt tgagggcttc
      421 cccagggttt tgttacaagc cctggagaag ccctcggcgg ccggcgcacg cgccgggctc
      481 cgaggtggat ccggtggcca tgaccggctg ttgcgaagat cggcggcccc gggtcggcaa
      541 gaacccttgc gggggcgaag cggttgcaac atacggcaac aactgtcgac cagaccacca
      601 tccgccagcc gatcgactgc accggcgtcg ggctgcacag cggcgccgtc gcgcgcatga
      661 tgctgtgccc ggccgaacgc gacaccggca tcgtgttcgt gcgcgccgat ctcggcggca
      721 gcgagatccc ggcacgctac gaccatgtcg tggacacccg cctcggcacc accctggccg
      781 ggcccgatgg cgcgctcgtg cacacggtcg agcacctgat ggcggcgctg tggggctgcg
      841 gcatcgacaa cctcgtcgtc gagctcgacg gccccgaggt gccggtcatg gacggcagcg
      901 ccgagccgtt cgttttcctg gtcgaatgcg cgggcatccg cacgttggga gcggcgcgcc
      961 aggcggtgcg ggtgctcagg ccggtgacgg tcacggacgg cgacacgcgc atcgcgctgg
     1021 ttcccgacga ccgcacctcg gtgcggttcg agatcgcctt cgacaacccg gtgatcggca
     1081 accaggtgct cgacctcgcc tgcgacgact gcgcgttcaa gaccgatctc gcgcgcgcgc
     1141 gcaccttcgg gctcgaatcg gacgtcgccg ccatgcacgc caacggccgc ggcctcggcg
     1201 gcagcctggg caatgcggtg gtggtcggca aggaccgggt gctgaacgag ggcgggctgc
     1261 gcttcccgga cgagttcgtg cgccacaaga ttctcgactg cctgggcgac ctgtatctgg
     1321 ccggggcccg aatcgtcggc cgcatcgagg cggtgcgcgc cggccatcgg ctcaacaacg
     1381 ccctgttgcg cgcgctgttc gccgagcccg cgaactggtg tctggaggcg gcggcgccgc
     1441 gcacctggac cgacgacgcg gtggcggcgc tggcctgacc cgcgtgcccg cggcgccggc
     1501 tggtgagtcg gcggcggcca ggcgctatag tggtcccatg agcgatgctg ttgcgcccgc
     1561 ctttgagctg gccggccgcc gggtgtgggt ggccgggcac ggcggcatgg tcggcgcggc
     1621 gctgtgccgg cggcttgcgc gtgagcgttg cgagctgctg agcgtcggcc gcgaccgggt
     1681 cgacctcagg cgccaggccg aggtcgagga ctggatggcc gcggcgcggc ccgcggccgt
     1741 gttcgtcgcc gcggcgacgg tgggtggcat cctcgccaac gacagccgcc cggccgagtt
     1801 cctgtacgac aacctggcga ttgagactaa cgtcatcgag gccgcccgcc gcaccggcgt
     1861 ggccaagctg ctgatcctcg gctcgacctg catctatccc aggctcgcgc ctcagccgat
     1921 gcgcgaggac agcctgctca ccgggccgct cgagccgacc aaccagtggt acgcgctggc
     1981 caagatcgcc ggcatcaagc tgtgcgacgc ctatcgccgc cagtatggtt gcgacttcat
     2041 ctcggcgcag ccgactaacc tttacggccc gcacgataat ttcgacctcg cgagcagcca
     2101 cgtgctgccg gcgctgatgc gcaagatgca cgcggccaag ctcgccggcg ccgatacggt
     2161 caccttgtgg ggcagcggca cgcccaggcg cgagttcctg tatgtcgacg acctggccga
     2221 tgcattggtg ttcctgatgc gccactattc caccgaaggg ccgatcaatg tcggcaccgg
     2281 cgaggagctg agcatccgcg agctcgccga gcggatcgcg gccgtggtcg gcttcgccgg
     2341 gcgcttcgcc tacgacacca ccaagcccga cggcacgccg cgcaagcttg ccgacgtggg
     2401 caagctcgcc gctctcggct ggcgcgcgcg gacctcgctc gacgacggcc tcggcctcac
     2461 ctatcgctgg ttcctcgacc acgtcgcgga ccggcttgcg gcggccgccg ccggctgacc
     2521 ggcgcgtgcc cgatcccgcc tcgcatctgg tcgacggcgt cggcctgtgg cgcgagctct
     2581 tgcgcgcccc gcccgccggc ccgccgcgcc cggcgctgtt cgtcgaccgc gacggggtgg
     2641 tgatcgagga tgcagggttc gtccaccgcc ccgaccaggt ttcgttgatc gccggcgcgg
     2701 cggcggcgtt cgcgtgctgc aatcgcgccg gcgtgccggt ggtgatggtc tccaaccagt
     2761 cggggatcgg gcgcgggctg tacggctggg cggagttcgc cgccgtgcag gcgcgcatgg
     2821 cggcgctgct cgccgccgcc ggcgccgcgc tcgacatggt gctggcttgt gcctatcacg
     2881 cggacggtgt gggcgcgctc aaggtcgccg gccacgactg gcgcaagccg cgcccgggca
     2941 tgttgtgcgc ggcggccgag gcgtggccga tcgatctcgc gcgctcgtgg atcgtcggcg
     3001 accggtcgcg cgatctcgcg gcgggacgcg cggcggggct cgccggcggc gtgctggtcg
     3061 ccaccggcca cggcagcgag tcaagcgagc gcgacgcggc gcaattgcta gccggcgaac
     3121 gattcgaggt gcgcgggacg cgctccatcg ccgccatcga cgcggtgctg gcgcggctcg
     3181 gcgctgggct cgagccggcc aggcgcgcgc gatgacggtc ctcgtcaccg gcgctgccgg
     3241 cttcatcggc ttccacgtcg cctccgcgct gctcgcgcgc ggcgagtcgg tggtcggcgt
     3301 cgacgacctc aacccctatt acgacgtcgc cctcaagcaa gcgcggctcg cccaactcga
     3361 aggccgggcg gggttccggt tccgccggct cgacatcgcc gacgcggcgg cgatggagca
     3421 gcttgcgcgc gccgaagggc cgttccggcg catcgtccat ctggcggcgc aggccggcgt
     3481 gcgccattcg ctcacccagc cgatggccta cggccgcagc aacctggtcg gccagctggt
     3541 ggtgctcgag ctcgcccgtc ataccgaggg cttacggcat ctggtgttcg ccagctcgag
     3601 ctcggtctac ggcggcaacg ccaagctgcc gttcgcggtc gccgaccggg tcgacacccc
     3661 ggtctcgctc tatgccgcga ccaagcgcgc cggcgagctg atggcgtaca gctattgccg
     3721 cctgtacggg ctgccgacca ccgggctgcg cttcttcacc gtctacgggc cgtgggggag
     3781 gcccgacatg gccgcctggc tgttcaccga ggcgatcctc gccgggcgcc cgatcaaggt
     3841 gttcaaccac ggcgacatgc gccgcgactt cacctatgtc gacgacatcg tcgacggcgt
     3901 cgtgcgcgtg atcgaccgcc cgccgcccgc gggcgaggcg ccgccgtggc acctctacaa
     3961 catcggcaac caccgccccg agccgctgct gcgcttcatc gccgtgctcg aggccgcgct
     4021 cgggcggcgc gccgagaccg agatgctgcc gatgcagccc ggcgacatcg ccgagaccta
     4081 tgccgacatc gatgagctga gccgcgacac aggcttccgc ccgaccacca cgatcgaaga
     4141 gggcatcccg cgcttcgtcg cctggtaccg cgaccattac ggcgtgtgag cggcggcgcc
     4201 gaggcccagt cgctcgccgc cgtagacgcg ctcgcgcagc ggccgccacc acgcctcgtt
     4261 gtcgaggtac cagcgcacgg tgcgggccag gccgtcgttg aaatcgatct cgggccgcca
     4321 gccgagatcg gcgcgggcgc ggcccgcgtc catggcgtag cgccggtcgt ggcctgggcg
     4381 gtcggcgaca cgggtgatca ggcgctcgcg cgggttgtgc ggcgagcccg gatcggcggc
     4441 gtcgaccagc cggcagatcg cccgcaccac gtcgatgttg cgccgctcgc agtccgcccc
     4501 gatgttgtag gtcgagcccg gacgcgcgcg ggtcagcacc gcggccagcg cgcgcgcgtg
     4561 gtcctcgacg tgcagccagt cgcgcacctg gccgccgtcg ccgtagaccg gcagcgcccg
     4621 gccctcgagc gcgttcagga tggcgagcgg gatcagcttc tcggggaact ggcgcggccc
     4681 gtagttgttg gtgcagttgc tgactaccac cggcaggccg taggtgcgct gccaggcgcg
     4741 cgccaggtgg tcggccgccg ccttcgacgc cgagtagggc gaggacgggg cgtagggcgt
     4801 cgcctcggtg aagcgcccct cgggcccaag cgcgccgtag acctcgtcgg tcgagacctg
     4861 atggaagcgg aagcgcgcgc ggcgtgcatc gtcgagacct tgccagtgcc ggcgcgccgc
     4921 ctcgagcagc acataggtgc cctcgacgtt ggtgtgcagg aagccggcgg gggcgtcgat
     4981 cgagcggtcg acgtggctct cggcggcgag attgacgatg gcgtcgggat cgtgggcgtc
     5041 gaacgcggcc gccactgccc gcgcatcgca gatgtcggcg tgcacgaacc ggtagtttgg
     5101 gtgatcggcg acctcggcga gcgaggccgg gttggcggcg taggtcagct tgtcgaggtt
     5161 gaccacctcg tgcccggcgt cgcgcagcag gtggcggacc agcgccgagc cgatgaagcc
     5221 cgccccgccg gtgaccagga ccttcaccgc gccgcccctt gcgcgcgccc ggcggcatag
     5281 gcgaacgggc tctcgatctg gtcgagccgc ggccaggacc ggtcgcgctc cgagacctcg
     5341 gcgtccgcgg gcgccaccgg ccagcgaatc gccagcgccg ggtcgtgcca caggatgccg
     5401 cgctcgtgct cgggcgcgta gacgtcagag accttgtagg cgacgcgggt gtcgggctcg
     5461 agggtgcagt agccgtgggc gaacccgacc ggcaccagaa gctgctcgcc cgccgccgcg
     5521 gtcagggtca ccgccacgtg gcggccgaag gtgggcgagc cgccgcggat gtcgagggcg
     5581 acgtcgagca ccgccccggt gaccacgccg accagcttcg cctgcgccgc gggcgcttgg
     5641 aaatgcagcc cgcgcacggt gccggcggcg cgcgagtagg ccaggttgtc ctggacgaag
     5701 tcgatgtcga tgccggcctc ggccagcgcc cggcggctgt aggtctcggc gaagaagccg
     5761 cgggcatcgc cgaatcgttt cggcgtcagg cggatcacct cggggatggc agtcgccgcg
     5821 atgtccacgc cgtcgaagtc ctccggggcg gtctggcccg gctctggtag catcggcaag
     5881 atagcgacgc aagccggcgg ccctgcccgc gccctcggtt cggccttgcc gggcgggggt
     5941 cgcgcggtca ccttgtaatg cggcatgcca cgatcggatc tggatgttgg tcaaggacgc
     6001 gtcgggcccg cggccggcac cgtacctgcc gacacgccgg cacagggtcg tgacccggcc
     6061 atgagttccg cccggcgccc gagcgcccgc acctgggcgc gcctcggcgt ggccgcgctc
     6121 gcggcgctgc tgctttatct cgccttccgc ggcgtcgact gggcggagct cggggcgctg
     6181 ctcgccggcg ccgatccggc gcgcgtcggc ctcgccgtcc tgatcatgac cgccggcacc
     6241 atgctgcgcg cccagcgctg gcgcctcttg gtcgcggcga gggtcgagcc cggctacccg
     6301 atcatgttct gggcgaccat ggccggctat gtcggcaacg cctatctgcc ggcgcgcgcc
     6361 ggcgacctgc tgcgctcgct cctgctcggg cgccacctcg gcatccgcaa gagcttcgtg
     6421 ctcggcacca cggtcaccga gcgggtgctc gacaccatcg tcatcgtggc gctggccggg
     6481 gcggtggtcg cgctgctgcc ggaggcgccg cactggctgg cctcgtcgtc gaccggcctg
     6541 tcggtgggcg cggcgtgcgc gctggtggcg ctgctcgcgg tgccgcggct cgccggctac
     6601 atcgaaggct gggtcgcccg gttgccgatg cgccgcgaat ggcgggcccg gctgatcgag
     6661 atctggcacg agttcttcac cggctcgcgg gcgatcgaga accacgtcac cgccgccgcc
     6721 ttcgccgcgc tcagcgtgct gatctggctc atggacacgg cgtgcatgac caccgtcgcg
     6781 gcggcgctcg ggctccatat gatcacgccg ctgctcgcct tgttgctggt ggtggcgctg
     6841 gcgctgtcga gcgtcgcgcc ctcgaccccg ggctatgtcg gcgtctacca gttcgtcgcg
     6901 gtgagcgtct tggcgccgtt cgcggtcggc cagagccagg cgctggcgct ggtgctgctg
     6961 ttccaggcgg tgatctacgt cgtggtgacg ccgtggggcc tgatcgggct gtggcggctc
     7021 ggcggcctgg gtgcggcgcg cgccggcccg tgaccccggg cggcgcgggc ggaaaggcga
     7081 tgacgggggc gctggctgag tatctggacg cggccgccgg cctgttgcgc gaaaccgccg
     7141 gcgccgggct cgaccggccg gtcgcggcgg cggtggcggc gatctcaacc gcgctcgccg
     7201 ccggcaagcc gctgctggtg tgcggcaacg gcggctcggc ggcggacgcg atgcacatcg
     7261 ccggcgagct ggtcgggcgc taccggctgg agcggccggg gctgaaggcg atcgcgctcg
     7321 gcaccgacgc cgcggtgacc accgcctggg ccaacgacta cgacttcgag accgtgttcg
     7381 cgcgccaggt cgaggcgctg ggcgaggccg gcggcgtggt ttgggcgatt agcaccagcg
     7441 gcgagtcggc caacgtcgtg cgcgcgctcg aggccgcgcg cgcgcgcgcc atgaccacgg
     7501 tggcgttgac cggcgccggc ggcggccggc tggcggcgct cgcggacgtg ctgatcgcgg
     7561 tgccctcgcg cgacaccccg cgcgtccagg aggtgcacac ctgcctctac cacttcatct
     7621 gcgagcaggt cgaacggcgc atcgccggcg cggcgggctg agccctcagg tgccggcgag
     7681 ccggtcgagg caggcggcga ggccctgacg ccaatcgggc tgggcgaggc cccagacggc
     7741 ggcgatgcgg gcgcagtcga gcaccgagtt cgccggccgc cgggcggggg tcgggtggtc
     7801 ggcggtagcg atcgcgcgca gccgctgcgg cgccggcagg ccgcggcttg ccgcgccggc
     7861 gaagatctcc tgcgcgaacg cggagcggct caccggcggc gcgccgcagt agtggaaggt
     7921 gccggttccc ttgccgcccg cggccaagcc ggcggcgatc gtgagcgcgg cgtgggcgag
     7981 gtcgcgggcc gcggtcgggc cgccctgctg gtcgcagacc accgccacct cgtcgcgctc
     8041 ggcgccgagc ctcagcagcg tggtgacgaa gttgcgcccg aacgggctga acacccaggc
     8101 cgtgcgcagg atcgccgccg tcccgcccgc ggcgcgcacc gcgtcctcgc cggcggcttt
     8161 cgacgcccca tagacgccga gcggcgcgcg ggcatcgtcc tcgcgatagg gccggttggc
     8221 ggtgccgtcg aacacgtagt cggtcgacac gtggaccagc gggatgcgcg cggcggcgca
     8281 cgcctcggcg accgcgccgg cgccggcctg gttgaccgcg aatgcgggcg cgggctcgct
     8341 ctcggcgcgg tcgacgtcgg tataggcggc ggcggtgatt gccgcgcgcg cgcccgcgat
     8401 cgcggcgcgc accgcccggg gatcggcgac gtcggcctcg gcatgcgcca aggcgcggac
     8461 ttggaaaccg tcgggcgccg tcgcgcgggc cagctcgcgg ccgagctggc cgccgccgcc
     8521 gaataccacg accgttgctg ccggcatcgc cggcatcgcc ggcccggcct tactcgccct
     8581 cgaacgcgca caaggcgaac accttgtgcc ccgccttctc cagccgcgcc cgcccgccga
     8641 cgtcgggcag gtcgacgatg aagcagcatt cgaccacccg cccgcccgac ttctcgatca
     8701 ggttgaccgc ggcctcggcg gtccccccgg tggcgatcag gtcgtcgacc accagcaccc
     8761 ggtcgccggc ggcgatggcg tcggtgtgga cctcgaccgt gtcggtgccg tattcgaggt
     8821 cgtaatggtg ctcgatggta ttgccgggca gcttgccctg cttgcgcacc ggcacgaagc
     8881 cgacgccgag ctggtgcgcg atcggcgcgc cgaggatgaa accgcgcgcc tcgatcgcgg
     8941 cgaccttgtc gatgcccgcg ccggtgtagc ggttgaccag ctcgttgacc gcggtgcgga
     9001 agccgcgcgc atccgcgagc agggtggtga tgtcgcggaa catgatccct gccttggggt
     9061 agtcgggaat ggtccggatc aagtccttga tcggcatcgc tggctctccc cgcgggcagg
     9121 cttgttgcag gcgcggcgca gtctacaata gccgggcccg ccccggcact gcgcagcgat
     9181 cctcgtcctc cgtcaatccg agcgccgacg cccgccatgc acatacggac cctggctgtg
     9241 atcggactcg gctatgtcgg gctgccgctg gcggtggcgc tggcgcgcag ccgcaaggtg
     9301 gtcggcttcg acctgtccgg gcgccgcatc gccgagctca cggccggccg cgaccgctcg
     9361 ggcgaggtgc ccaacgaggc gctggccgcg gtcgagatcg cgttcaccgg cgaggtcgag
     9421 gcgcttaacg ggctcgatgc ctacctgatc acggtgccga cgccggtcga cggctcgaag
     9481 aagcccgacc tgtcggcggt cgagcgggcg tgcgcaaccg tcggcgccgc gctcgccccg
     9541 ggcgcggtgg tggtgctcga gtcgaccgtc tatccgggca cggtcgagga ggtgtgcgcg
     9601 ccgatcctcg agcgcgcctc gggcctcgcc gccgggcgcg acttcttcct cggctactcg
     9661 cccgagcgca tcaacccggg cgatgcggct caccaactgg gcgcgatcac caaggtggtc
     9721 gccggccaga cgcctgcggt caccgccgtg ctggccgagc tgtacggcgc catcgggccg
     9781 gtgttcgtcg ccgccgacat ccgcaccgcc gaggccgcca aggtgatcga gaacgcccag
     9841 cgcgacatca acatcgcctt cgtcaacgag ctggcggtga tcttcggccg gctcggcctc
     9901 gataccggcg cggtgctcga cgccgcggcg accaagtgga acttcctgcc cttccggccc
     9961 ggcctggtcg gcggccactg cgtcggcgtc gatccctact acctcaccta cgtcgccgag
    10021 cgctcgggct accatcccga cgtgatcctg gccgggcggc ggatcaacga taccatgggc
    10081 cagcatgtcg gccgcgaggt ggcgcggcgc gtgcatgcgc tcgccttgcc caggcgctgc
    10141 ctggtgctgg gcatcacctt caaggagaac gtccgcgaca tccgcaactc gggcagcgcc
    10201 ggggtggcgc gcgagctggc ggcgttcggg ctcgaggtcg acgtccacga cccgctcgcc
    10261 gatgccgacg aggtgcgcgc ggcctacggc ttcgagctgg tcgcgagtcc cgccggcggc
    10321 tacggcgcgg tggtgctcac ggtcggccac cgcgcctatg ccggctggcc ggcgagcgag
    10381 gtgcgggcgc tgctcgccgg cggcggcctg gtcgccgacg tgcgcggcat ctggcgcggc
    10441 ctcgatttcg gcgacgacat acaggtctgg cggctatgat ggccaaccac ggcgccgatt
    10501 ggtaagaaag tcggcaccag ggcgaaagac aagcgcgtcg cggcaagacg cgcgggagag
    10561 ggaggacgta ccggtgaaga gcgggcggaa cgggttcgtc agtcgggctt gccggctggc
    10621 cggcgcggcg ctggtgtgcg tcggcgccgc ggcgctggtg gcgtacggtc cggcggcggc
    10681 gcaggaggag ggcgaggcgg tctctccgga cagcctcaag cacggcaaga tcacgttccc
    10741 gagcggcagc gtctacgagg gcgacctcag cgacgggcgc atgcacggca cgggcaagat
    10801 cgtctacgcc gacggcggcg tctacgaggg cagcttccag gacgacaaga tcaccggccg
    10861 cggggtgttc gtctacccca acggcgaccg ctacgagggc agcttcgtcg acggccagcg
    10921 ccacggcgac ggcgtgttcg cgttcgccaa cggcgccaag tacgagggca gcttcgaggg
    10981 cggccagatg cacggccagg gcacgctgac cttcgccaac ggcgcggtct acaccggtgc
    11041 cttcgtcaac ggccggcgcg agggcgaggg caagctcagc ttcgccagcg gcgacgtctt
    11101 taccggcacc ttcaagggcg gcgagatgga cggcaagggc cggttgcagt atgccgaggg
    11161 ccacgtctat gagggcgagt tcaagggcgg caagcgccac ggccagggca ccttccgcat
    11221 gagcaacggc gacgcctatg ccggcgagta caaggacggc gagcgcaacg gccgcggcac
    11281 ctacacccag gccgacgggg tcaagtatgt cggcgactat gccgacggca agcgccaggg
    11341 cgagggcagc atcctgtacc cgaacggcga ccgtttcgag ggcaagttcg cggccgacct
    11401 gcagcagggc ccgggcaagc tggtctacca ggacggcacc caggtcgagg tcacctacga
    11461 ggccggcaag ccggtcggca aggcggtctg gatctacccc gacgggcacc gcatcgaggg
    11521 cgagttctga ccgcgccgcg attggtcggg acggcacgcc gcgcctatat tcgaatgttg
    11581 aaggaggcgc cgcggcgagg atggcaggcg gtggtcgcct gtaatgccgt tccggggcgg
    11641 ttggatgcag caagccgtgg caatcgaatc gcagtcgccg ggacggagat cgggacgccc
    11701 ggctgacccg ggcggcgagc tgcgccgcca gctcgaccag gcccaccggg cgatgtgggc
    11761 gctctacaag gagctggacg acaagaacgc ggcgctcaac cgctccaacg aggagctcga
    11821 gcagttcgcc accatcgccg ggcacgacct ccaggagccg ctgcgcaagg tgatcgcctt
    11881 cgccgcgctg ctgcgcgagg agcacggcca gggcctcggc gccggcggcc gcgactacct
    11941 cgcgcgcatg gacggggcgt gcgagcgcat gctggtgctg atcgaggacc tgctgcggct
    12001 ggcgcgcgtc accagccagg cgcgcccgtt cgagcgctgc gatctcgccg ccgtcgccgc
    12061 cgaggtggcg gccgacctcg gggcccaggt cgagcgcgag cgtggcgagg tggtgatcgg
    12121 gccgctgccg gtgatcgagg ccgatccggt ccagcttcgc cagctgctcc agaacctggt
    12181 ctcgaacggc ctcaagttcc accacccgga ccggccgccc agggtcacgg tctcggtgcg
    12241 ccggctcgac ggcgatcgca tcgaggtcca ggttgccgac aacggcatcg gcttcaaggc
    12301 caagttcgcc gagcgcatct tccagccgtt ccgccgcctg cacggccagg gccgctaccc
    12361 gggcaccggc atgggccttg cgatcgtgcg caagatcgcc cagcgccacg gcggcgaggt
    12421 gcgggtcgag agcaccctag gcgaaggcag caggttcgtg gtcgcgctgc cgtgccggca
    12481 cggccgccag gccgccggcg agtccgcgag ggccgccggc gatggccgct gagcaccccc
    12541 ggcgcaggtc gagcgacctg ccccggctgc gcattcttct ggtcgaggac aacccggacg
    12601 acgtggtcct gttcggcgag ctgttgggcc gcatcgcgcc gctgagccgc ttcgaccacg
    12661 tcgattcgcc ggacgaggcg cgccggcggc tgcacggcgc gggctacgac ctgtgcttct
    12721 tcgattacga gctcggcgac gagaccggcc tcgacctgct gcacacggtg cgcgccgacg
    12781 gctacgaccg gccgatcatc ctgctgaccg gccaaggcga cgagaccgtc gcggtcaagg
    12841 cgatgagggc cggggccagc gattacctgt ccaagcacgg cctcagcgtc ggcgtcctcg
    12901 gcggcgccat ccgccacgcc atgcagctgt acgagaccga gcaacggcgc cagcggttcg
    12961 aacgcgcgct gcgcctgtcc gagcaacgct accgcgagct ggtcaaccgg ctgccgctgg
    13021 tggtcggcga gctggccgag accggcgagg cgcggttcgt caacgaggcc gcgagccagg
    13081 tcaccggcta tgccgcggac gagctggtcg gcagggactg gtggcgcctg cttgcgcccg
    13141 atctcgacgc cgccgagctc gacctgctgc gccagagggt gctcgcggag ggcctgaacg
    13201 cgatcgagat gccggcgcgc cacaaggatg ggcgcatgct gaccatggag tggtcgacgg
    13261 cggtgatgcg ccaggccgaa ggcggcggcg agaccatcgt ctgcatcggc atggatgtga
    13321 ccgagcgcgc gcgcatgcgc gacgagctga tgtggctggc ggtgcgcgac gagctgaccg
    13381 gcttgagcaa ccggcgcggc ttcctgacgc tcgccgagca gcagctcaag gcggcggcgc
    13441 gcgacaagca ctcgatgctg gcggtgttcg tcgacctcga cgggctcaag tcgatcaacg
    13501 accagctcgg ccatgccgag ggcgaccgcg cgctcagcga catggccgag gtgatgcgcc
    13561 gcacctttcg cggctgcgac gtgatcgggc gcatcggcgg cgacgagttc gtggcgctgg
    13621 tcaccgaggg ctcgccgttc cgctcggacg gcatcatcca gcgcctgcag accgtgctcg
    13681 ctcagttcaa cgccgacgag acccgccgct attacctgtc ggccagcatc ggcgtcgcgc
    13741 gctacgatcc ggacacgccg atcgcgatcg atcatctggt caaccgcgcc gactcggaga
    13801 tgtacgagaa caagcagcgc cgccggcgcc gcgacgtcgt gcgcaaggca accccggtgg
    13861 ccgccggctg aaggggcccc gggcgcggcc tgcgccagga gaccgccatg gcgaacctgt
    13921 cgcgttccaa gtcacttggt cgtgccgtcg gcgcggcgct cggcgccgtc gttgccgccg
    13981 cgccgggcgc cgcctgggcg tgctgccacg gcggcggggg cggcggcggc gcctcggtgc
    14041 cgccgagcat gccgccggtg atgccgccca tgatcgtgcc gtcggtgcct gccgcgccga
    14101 ccgcggtgca ggacgccgtc cgcgacgcgc tccgtcccga cacgccccgg cgcgagccgt
    14161 cgataccgga aatgcgctcc gccgacccgc ggccctcgat accggagatg cgcgccaccg
    14221 acccggccga ggtcgagcgc atggagcgcg aggaggcggc gcacgacgag gcgcgccggc
    14281 gccagaccga gcgcgacatc gagcgctggg aggaatggcg cgaccgcggc aaggagatca
    14341 aggtcgtcgt cggctgggcc aacaacgtcg ccggcggcgc cgccatcgcc gcctgcgcca
    14401 ccggcgtcgg ctgcatcccg gtgctgatct atgccggcgg caagttgatc gaggtgctgc
    14461 tggtcagcca cgccgaggcc tcgcgcggca ccggcttcgt cgagcgcctg gtcaacggca
    14521 gcgcgagctc cgccgactac tggcggcggg tgcgcgtcaa cggcttcggc accatggcca
    14581 gcgaggcgat cggcctggcc ggcgatgccg tcggcctgcc cggcgtccat tccggcggca
    14641 tcggctcgat gacccaggac gcgatgggcg agagcctcaa cgccggctac gagtcgatcc
    14701 gcgacggcct gcacgagcag aacgagcgcc agcgccagga gatctggcag cgccaggccg
    14761 accgcgcgac catggaggcg tacggcctca acaccgcggg cggcttcccg atgcccgcgg
    14821 gagcgccgta ccgatgaccg gcccgcgcgc gcgcgtcgcc ggtgccggcg cgctgctcgc
    14881 ggcgctggtg ttcgccgcgc tcgtgccggc ggcggcgcag gatgccggca cgccggccat
    14941 cgaccccgcg gcgcgcgccg ccctgtcggt gctcgaggac cgctacacgc ccgactccga
    15001 ggcggtggtg cgcgcgctcg ccgcgcgcct gcgcgggctt gccgaccgcc cgggcggcgg
    15061 gctcagcgtc gcctttaccg acccggcgcc gatcgaggcg gcggcgccgg ccttgcgcgc
    15121 cgacccgccg ctcgccgtcg acggcttcgc gatcaccgcc tacgccgccg acccggcgcg
    15181 cccgacccgg cacgcgctgc acggactgct gagcttcacc gaccgcttcc agcgccggct
    15241 cagcgtcacc ttcggcgccg actacgaggt cgcggacgac ttggtcacgg tcggcgcggt
    15301 cagcgtcgcg ccgtacttcc cgccggtgcc ggcggtggtc atgctgttcg tgcccgcgaa
    15361 ccgggtggcg gcggacttct ggacccgcca cgcgggactc gacgacctgc tcgatttcgt
    15421 cgccgagaat gccgtgagct tcgcgctgcc gtgggcggtg ccgcgcgtgc ccgaggacca
    15481 ctacgccatg gcgttcttct acgagcagct gccgcgcgcg gcccgcgtcg acctgctggt
    15541 cgccgacgcg cccgagggcc tgaccggcga gcggcgctcg accgtggtgc tgcgcgaggg
    15601 cggctggcag gtcgcgatcg cgcgcgtcca cctcgccttc gccgagcgcg ccacggtcta
    15661 cttcaaggcg ctctactggc cgagcgacga cgtgagtggc ccggaaggcg cggtgcgcat
    15721 cgccgaggtg ttctcgaacc gcatccccga ccttgcggcg cgcccgcgcc ccgcggtcga
    15781 agcgcccgaa ccggcgcccg cgcccgcggt cgcggcaccc gcggcgccgc ccgcgcctga
    15841 ggcgaccccg gcggcggccg ccgacagcgt cgagcagcgc ctcgagcgcc tgcgccagct
    15901 ctatcagcgc gagctgatca gccgcgagga atacgacgcc aagcgccagg ccctgctcga
    15961 ccagctctag ccgcgcggcc gcgcccgaca tgacccggtc cgagtcggag acctggcgcg
    16021 cctactatgc cgcgaccggg gcgcgggcgc cgcgcgcgac gctgctgatc gcgctcgacc
    16081 gattcgccgc cgagggcgcc gatgccggca agctccatgc gctcgacctc ggctgcggct
    16141 cgggccgcga tgcggtcgag cttctggcgc gcggctggtc ggtgctggcg atcgacgccg
    16201 agccggcggc gatcgaggcg ctgcgcgcgc gccccgagat caccgcgtcc gcacggtcgc
    16261 ggctcgagac ccgcgtcgcc cgcttcgagg ccgcccggtg gccgcgggtg gacctggtca
    16321 acgcgagctt cgccctgccg ctggtgccgc ccgccgcctt cgccggcctg tggcggcgca
    16381 tcgtcgcctc gctcaagccc ggcgggcgct tcgccggcca gctctacggc gagcgcgacg
    16441 gctgggcggg cgacccgacc atcacctttt tcagccgcag ccaggtcgag gcgctgctcg
    16501 ccggcctcga ggtcgagcat ttgcttgagg aggaggacga cagcgtcacc ccgcgcggcg
    16561 cgcccaagca ctggcacatc ttccacgtcg tcgcccgcaa gccgcacgat cgcgcgaaag
    16621 aaggatcaac cacagacagc acaaaggaca cagcggcagg ctgacccggc gccggcaacg
    16681 gcggggcgcc gcacccccac ccataccctc ccccgcccgc gggggagggc tttcatacca
    16741 gcgagcgact gaaacaactc gctgtatttc gtcgtcatcg cgagcgaagc gtggcgatcc
    16801 agcacgcgat caccgcaggt ctggatcgct tcgtcggcat cgcctcctcg cgatgacgcg
    16861 cgactatgag atgcaatcgg gtcggatcaa gcgctcgctg gtatcaagct cgaacctccc
    16921 cccgcttgcg gggggaggaa ggttgggggt cgcacggact cgacgcgaac gggattcgcg
    16981 cgggcgcgcg caatcactcg catggcaatg ataagccttc ttcctctgtg ccctctgtgg
    17041 tgaattccct tcttttcctc cgctcagcgc atcgcgaacg ggttcgggaa ctcggcgttc
    17101 atgtcgaccc agacgctctt ggtctggagg tagtcgcgga tcatgtcctg gccgttctcg
    17161 cggcccatcc ctgagcgttt gtagccgccg aacggcgagg tgaacgagat cgcgcggtag
    17221 gtgttgatcc acaccgtgcc cgcctgcagc tccttggcca tgcggtgggc gcgggtgatg
    17281 tcctgggtcc acaccccggc ggcaagcccg taggcgacgt cgttgccgat cgcgatcgcc
    17341 tggtcgtcgt cggtgaacgg gatcaccgac agcaccgggc cgaacacctc ttcctgggcg
    17401 atgcgcatgc ggttgtcgac gccggtgaag atggtcggct cgacgaacca gccctcgccg
    17461 cattcgggcc gctgggccgg gcgcccgccg aggcggagct cggcgccctc agcactcgcg
    17521 atgccgatgt agtcgagcac cttctggtac tgcggcgggg tggtcacggg gccgacctgg
    17581 gtgtccatct ccatcgggtt gcccatgcgc gcgcccttgg cgaagtcgac caggcggtcg
    17641 acgaagcggt tgtggatctg ctcctggacc aggaggcgcg agcccgcgat gcaggtctgc
    17701 cccgttgcgg cgaagatgcc ggcgaccgcg cccttgaccg cgttgtcgag gttggcgtcg
    17761 gcgaacacga tgttgggcga cttgccgccg agctcgaggc cgaccttctt gaacccgggt
    17821 gccgccatct cgttgacctt ctggccggtg cgctccgagc cggtgaacgc caccttgcgc
    17881 accaacggat gctcgaccaa cggttggccg acctcagccc cgaagccggt gaccacgttg
    17941 accacgccgg gcgggaaccc cgcctgctcg aacagcttga cgaactcgag cacgctggtc
    18001 gaggtgaact ccgacggctt ga
//
LOCUS       DASWHU010000203         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2335283, whole genome shotgun sequence.
ACCESSION   DASWHU010000203 DASWHU010000000
VERSION     DASWHU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335283"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1361)
                     /gene="hemA"
                     /locus_tag="VGB88_07340"
     CDS             complement(150..1361)
                     /gene="hemA"
                     /locus_tag="VGB88_07340"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX9647297.1"
                     /translation="MDYERFFAEALDSLRKEGRYRVFADLARHAGNFPTATCYNDGVE
                     RRVTIWCSNDYLGMGQHPKVVEAMIEAARRAGAGSGGTRNISGTTHYHVMLERELADL
                     HHKEAALLFTSGYISNEATLSTLASMLPGCVIVSDALNHASMIQGMRHSRAPKKIFRH
                     NDVAHLDRLLGELRPDAPKIVAFESVYSMDGDIAPIADFCDLAERYGALTYLDEVHAV
                     GLYGPRGAGIAAREGVMGRVSIIEGTLAKAYGCLGGYIAGSAALCDFVRSFAAAFIFT
                     TALPPHVAAGALASLRHLKVSEAERMQHQAKAARVKRLLREAGLPVMPSASHIVPLLV
                     GDAALCKQASDLLLDRHGIYIQPINYPTVARGSERLRITPSPQHSDAAIAHLVAALQD
                     VWCALGLQRAA"
     gene            complement(1590..2699)
                     /locus_tag="VGB88_07345"
     CDS             complement(1590..2699)
                     /locus_tag="VGB88_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647298.1"
                     /translation="MKAGGIIGGLVVGLVAGLGVGVYVIGGGGGEPAMQGAEPAADAV
                     PAAKAVRWKMASAFGSQWPIQGDLGMRFVDKVDAVSGGTLKIQFFEPGALVPALEIFD
                     AVSSGSIDAGWTTAGYWAGKVPALQFFTAVPFGPDGMEYAAWLYYGGGDELYNEIYQP
                     YNIKAMHCGMHSPEGSGWFRKPIETLDDLKGLKMRFFGLGARVMEKVGVSTQLLAGGD
                     IFPALELGTIDATEYSMPVIDLNQGFWQIAKHYYFPGWHQPSSIFELMINRARWDALS
                     PQQQEIIEIVCGDNYRAGLGMSEAAQAKALTELRAKGVEFHRWPDETLDVFRNAWAEV
                     AEEEAAKDPMFRKVLDSFNSFRAEYKVWADLGYAR"
     gene            2891..3874
                     /locus_tag="VGB88_07350"
     CDS             2891..3874
                     /locus_tag="VGB88_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX9647299.1"
                     /translation="MHASWFEDYGPAAEVLKVGERETPEPGAGEVRVHVKASGVNPSD
                     VKKRAGQQPALDDGWVIPHSDGAGVIDAVGRGVPSARIGERVWVYQAQYQRRFGTAAQ
                     YVCLPSSRAVRLPDRASFEVGACMGIPAMTAHRCVLADGTVAGQAVLVTGGAGRVGHY
                     AIQWAKWAGARVVATAGSEASAGHALAAGADAVVNYRTGDVAAAVLEATDGARIDRVI
                     DVEFGANLETTLKLITTGGVIATYASLREPEPRLPFYRMMRADIMLRCVLVYAMPEQA
                     KRLAAADITRFLDEGRLLHRVAETYALADIANAHEAVESGKVQGCVVVQLP"
     gene            complement(3893..4864)
                     /locus_tag="VGB88_07355"
     CDS             complement(3893..4864)
                     /locus_tag="VGB88_07355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9647300.1"
                     /translation="MKPAVFVHSWVPEPILADLERRFEVDHVDVFKTGVLPPDEFARH
                     AKGKQGLMVLTAPVPGELLKASAGTLKVVAAVAVGVDNIDVAAATAAGIQVTNTPGVL
                     TDAVADMSIAMVLALGRRLVEADRYTRAGKFGGSSFPLFWGATVVGETLGIIGMGRIG
                     QAVARRARGFGLKLVYHNRNRVDQAIEAELGATWLPLEGLLAAARYVLLLTPLSDQTR
                     HLINAQRLKAMRKDAFLINVSRGPVVHEAALLDALKQGAIAGAALDVYEFEPEITPGL
                     AELDNVVLTPHIASGTVETRHAMVRVAAENLIACLSGAPAPNPVNRV"
     gene            complement(4864..>5248)
                     /locus_tag="VGB88_07360"
     CDS             complement(4864..>5248)
                     /locus_tag="VGB88_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9647301.1"
                     /translation="GAGLAAMIDLVCAGLTGMAHGFRQPNFVATGTIPVPIGHCFIVI
                     DPSRIGAGAGFAERARALVDDLRAQPARPGQRVMAPGDPELAHAAERARTGIPLDPVT
                     WQAFADLAAKWRIAPPAAEISKGRG"
BASE COUNT          780 a         1811 c         1887 g          770 t
ORIGIN      
        1 gggtggcagg gtcggcaagc ggtggcggac agctttttta tagttacaca aattataatc
       61 agctcaataa ataccggccg tcaatggagg aaaggaacga ccagcccgtc aggcgcgcag
      121 tcctagcgca gcgcggcgcg ccacacgggc taggcggccc gctgcaggcc gagcgcgcac
      181 cagacgtcct gcagggcggc gacgaggtgg gcgatggccg cgtcgctgtg ctgcgggctg
      241 ggcgtgatgc gcagccgctc gctgccgcgc gccaccgtcg ggtagttgat cggctggatg
      301 tagatgccat ggcggtcgag cagcaggtcg ctcgcctgct tgcagagcgc ggcatcgccc
      361 accaggaggg gcacgatgtg gctcgcggag ggcatcaccg gcaggccggc ttcgcgcagc
      421 agccgcttca cgcgcgcggc cttcgcctgg tgctgcatcc gctccgcctc gctgaccttg
      481 aggtgacgca ggctggcgag cgcgccggcg gccacgtgcg gcggcaaggc ggtggtgaag
      541 atgaaggctg cggcgaagct gcgcacgaag tcgcagagcg ccgccgagcc ggcgatgtag
      601 ccgccgaggc agccgtaggc cttggccagc gtgccctcga tgatgctgac ccgccccatc
      661 accccctcgc gcgcggcgat gccggcgccg cgcggtccgt agagcccgac ggcgtgcacc
      721 tcgtcgagat aggtgagcgc cccgtagcgc tcagcgagat cgcagaagtc ggcaatcggc
      781 gcgatgtcgc cgtccatcga gtagaccgac tcgaaggcga cgatcttggg cgcgtcgggg
      841 cgcagctcgc cgagcagccg gtcaaggtgc gcgacgtcgt tgtggcggaa gatcttcttc
      901 ggcgcccgcg aatggcgcat gccctggatc atcgaggcgt ggttgagcgc gtccgagacg
      961 atcacgcagc cgggcagcat cgaggcgagc gtgctgagcg tcgcctcgtt cgagatgtag
     1021 cccgaggtga acagcagcgc cgcctccttg tggtgcaggt cggcgagctc gcgctcgagc
     1081 atgacgtggt agtgggtggt gcccgagatg ttgcgcgtgc cgcccgagcc ggcgccggcg
     1141 cggcgcgccg cctcgatcat cgcctcgacc accttcgggt gctggcccat gccgaggtag
     1201 tcgttgctgc accagatcgt cacccggcgc tcgacgccgt cgttgtagca ggtggcggtg
     1261 gggaaattgc cggcgtggcg ggcgaggtcg gcgaagaccc ggtatcggcc ctcttttctc
     1321 agggaatcaa gggcttcggc gaaaaacctc tcgtagtcca tggtcgcgcc catcgcttgc
     1381 ttcgcgctcc aagggtaccc caacgccgtt ctaacacaat ccagggacaa cacaatttga
     1441 agtcgtgcct tgacccaggt caaaggccgt cggcaaacgc gcgtgccgac ccgaaacccg
     1501 ccggcctgct cggtgccggc gggtcgctgg tgtcgaagcg caccggcgcc cgggcgagct
     1561 tcaggacgtg cctggggccg gccgcggtgt cagcgcgcgt agccgaggtc ggcccagacc
     1621 ttgtactcgg cgcggaagga gttgaacgag tcgagcacct tgcggaacat cgggtccttg
     1681 gcggcctctt cctcggcgac ctcggcccat gcgttgcgga acacgtcgag ggtctcgtcg
     1741 ggccagcgat ggaactcgac gcccttggcc ctgagctcgg tcagcgcctt ggcctgcgcc
     1801 gcctcgctca tgcccagccc ggcgcggtag ttgtcgccgc agacgatctc gatgatctcc
     1861 tgctgctggg ggctgagcgc gtcccagcgg gcgcggttga tcatcagctc gaagatgctc
     1921 gacggctggt gccagcccgg gaaatagtag tgcttggcga tctgccagaa gccctggttg
     1981 aggtcgatga ccggcatcga gtattcggtg gcatcgatcg tgccgagctc gagcgccggg
     2041 aagatgtcgc cgccggccag gagctgggtg gagaccccga ccttctccat cacccgcgca
     2101 ccgagcccga agaagcgcat cttgagcccc ttgaggtcgt cgagagtctc gatcggcttg
     2161 cggaaccacc cggagccctc gggcgagtgc atgccgcagt gcatcgcctt gatgttgtag
     2221 ggctggtaga tctcgttgta gagctcgtcg ccgccgccgt aatagagcca ggcggcgtac
     2281 tccatgccgt cggggccgaa cggcaccgcg gtgaagaact gcagcgccgg caccttgccg
     2341 gcccaatagc cggcggtggt ccagccggca tcgatcgagc ccgacgagac cgcgtcgaag
     2401 atctcgagcg ccggcaccag cgcgcccggc tcgaagaact ggatcttgag cgtgccgccc
     2461 gagaccgcgt cgaccttgtc gacgaagcgc atgccgagat cgccctggat cggccactgg
     2521 ctgccgaacg cgcttgccat cttccagcgc acggccttgg ctgccgggac ggcgtcggcc
     2581 gccggctcgg cgccttgcat ggccggctcg ccgccgccgc cgccgatcac gtacacgccg
     2641 accccgaggc ccgcgaccaa cccaaccacc aggccgccga tgataccccc tgccttcatg
     2701 gtgcgtgccc tcctccgatc ccggcgccgc gcagccgttc gcgcagcgtg cctgcccgac
     2761 ctgggccgaa ggtacccggt tgaagccatt cgtccaaggg gcgacggtgc cgggacatcc
     2821 ccggcttgcc ttcgccgcga accatccgta ggaaaggcgg ccgggacgga gccgaacggg
     2881 gaacatcgcc atgcacgcat cttggttcga ggactacggc ccggccgccg aggtgctcaa
     2941 ggtcggcgag cgcgagacgc ccgagccggg cgccggcgag gtgcgcgttc acgtcaaggc
     3001 gtcgggcgtc aacccgtcgg acgtcaagaa gcgcgccggc cagcagcccg ccctcgacga
     3061 cggctgggtg atcccgcaca gcgacggcgc cggcgtgatc gacgcggtcg ggcgcggcgt
     3121 gccgtcggcg cgcatcggcg agcgggtgtg ggtgtatcaa gcgcagtacc agcgccgctt
     3181 cggcaccgcc gcccagtacg tgtgcctgcc gtcgtcacgc gcggtgcggc tacccgatcg
     3241 cgcgagcttc gaggtgggcg cgtgcatggg catcccggcg atgaccgccc atcgctgcgt
     3301 gctcgccgac ggcacggtgg ccggccaggc ggtgctggtc accggcggcg cggggcgggt
     3361 cggccactat gcgatccaat gggcgaagtg ggccggggcg cgggtggtgg cgaccgccgg
     3421 cagcgaggcc agcgccggcc acgcgctcgc cgccggcgcc gacgcggtgg tcaactaccg
     3481 gaccggcgac gtcgccgccg cggtgctcga ggccaccgat ggcgcgcgca tcgaccgggt
     3541 gatcgacgtc gagttcggcg ccaacctgga gacgaccttg aagctgatca ccaccggcgg
     3601 cgtgatcgcg acctacgcct cgctgcgcga gcccgagccc aggctgccgt tctaccggat
     3661 gatgcgcgcc gatatcatgc ttcgctgcgt gctggtctat gccatgcccg agcaggccaa
     3721 gcgcctggcc gccgccgaca tcacccgctt cctcgacgag ggccggctcc tgcaccgggt
     3781 cgccgagacc tatgcgctgg ccgacatcgc caacgcccac gaggcggtcg agtcgggcaa
     3841 ggtccagggc tgcgtcgtgg tccagctgcc gtgaccgcga ccgccccacc cgtcacaccc
     3901 ggttgaccgg gttcggcgcc ggcgcgcccg acaggcaggc gatcaggttc tcggccgcga
     3961 cccggaccat ggcgtggcgg gtctcgaccg tgccgctggc gatgtgcggg gtgagcacga
     4021 cattgtcgag ctcggcgagg ccgggggtga tctcgggctc gaattcgtag acgtcgagcg
     4081 cggcgccggc gatggccccc tgcttgagcg cgtccagcag cgccgcctcg tgcaccaccg
     4141 gcccgcgcga gacgttgatc aggaacgcgt ccttgcgcat cgccttgagg cgctgggcat
     4201 tgatcaggtg ccgggtctgg tcgctgagcg gggtcagcag cagcacgtag cgcgcggccg
     4261 caagcagccc ctcgagcggc agccaggtcg cgccaagctc ggcctcgatc gcctgatcga
     4321 cccggttgcg gttgtggtag acgagcttca ggccaaagcc gcgcgcgcgc cgggcgaccg
     4381 cctggccgat gcggcccatg ccgatgatgc ccaaggtctc gccgaccacg gtcgcgcccc
     4441 agaacagcgg gaacgagctg ccgccgaact tgccggcacg ggtgtagcgg tcggcctcga
     4501 ccagccgccg gccgagggcg agcaccatcg cgatcgacat gtcggcgacc gcgtcggtga
     4561 gcacgccggg ggtgttggtg acctggatgc cggccgcggt ggcggcggcg acgtcgatgt
     4621 tgtcgacgcc gacggcgacg gcggcgacca ccttgagcgt gcccgcgctc gccttcagca
     4681 gctcgcccgg caccggcgcg gtcagcacca tcaggccctg cttgcccttg gcgtgacggg
     4741 cgaactcgtc gggcggcagc acgccggtct tgaacacatc gacatggtcg acctcgaacc
     4801 gacgctcgag gtcggcgagg atcggctcgg gcacccagct gtgcacgaag acggccggct
     4861 tcatcatccc ctccctttgc tgatttctgc cgccggcggc gcgatgcgcc acttggcggc
     4921 gaggtcggcg aacgcctgcc aggtcaccgg atcgagcggg atgccggtgc gtgcacgctc
     4981 ggcggcgtgg gcgagctcgg ggtcgccggg cgccatcacc cgctgtcccg gccgcgccgg
     5041 ctgcgcgcgc aggtcgtcga ccagcgcccg cgcccgctcg gcaaagcccg cgccggcacc
     5101 gatccgcgac gggtcgatga cgatgaagca gtggccgatc ggcaccggga tggtcccggt
     5161 ggcgacgaag ttgggctggc gaaagccgtg cgccatgccg gtcagcccgg cgcagacgag
     5221 gtcgatcatc gccgcgagcc ccgcgccc
//
LOCUS       DASWHU010000204         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1589674, whole genome shotgun sequence.
ACCESSION   DASWHU010000204 DASWHU010000000
VERSION     DASWHU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589674"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGB88_07365"
     CDS             <1..245
                     /locus_tag="VGB88_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647302.1"
                     /translation="TCCGMAGAFGYQAETAEVSLSIGELDLFPALRRPERAGALVVAG
                     GTSCRHQIADALGRPAEHAAVVLARALDRGRRARSC"
     gene            complement(247..999)
                     /locus_tag="VGB88_07370"
     CDS             complement(247..999)
                     /locus_tag="VGB88_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647303.1"
                     /translation="MPSAKRSWTSCSGARCAAGAAIGLALLVAAIPNAPPVHADIAAI
                     APPRATASAGPGPLTLDYHPGEFGDLRREVFLKQVFRGVRNVVRGVGRVVGEVAKGTV
                     KGAGEIGRAAAQVAQVPGEVGQKVGREVGGFVGGMVGGRRGRKVGRNIGGLAGTYYGG
                     KVASRSSLIETVNRAQGKAEQIAEMAARLDERRERIRRIFEGDIGGLAREELERRLPT
                     GVIQDIRPNVPTMLPPIRPTPSPPPVRVEGRF"
     gene            complement(965..2089)
                     /locus_tag="VGB88_07375"
     CDS             complement(965..2089)
                     /locus_tag="VGB88_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9647304.1"
                     /translation="MRLFARPSIGRLAALLALGAAPAAAAWPPSPETVALVMEAEAQE
                     LAEAVATEAEAAGGAAPDLVTELDAALAADPGNVEALNQRGLFRLELGDRQAALADFT
                     EAVRLAPKDPAFHHNQGLAHELLGKFSLAERAYRRARVSAAEDSEAGIAAWWALEVLH
                     NKPLLAQADILSRMGAPDSFTVAVSAQAGDPARLVRVEEWEYWRAGKAFTFVNAELRE
                     TRDIERPSGLVAYAPDRPYMFESGMAFLDAANAAGFEEYGYIEYDEPGLAGVDLVVAE
                     QLVLGFLDSRLFYAHSVPLRIAPFGPPRLPEVVPAAATEPTAPAPAPAAPAAPAAEPA
                     APDDTAELVRRLETLERLHDQGLITEEEYRAKRQAILDEL"
BASE COUNT          269 a          839 c          813 g          297 t
ORIGIN      
        1 cgacctgctg cggcatggcc ggcgcgttcg gctatcaggc cgagaccgcc gaggtttcgc
       61 tcagcatcgg cgagctcgac ctgtttcccg cgctgcgccg ccctgagcgc gccggcgccc
      121 tggtcgtcgc cggcggcacc agctgccgcc accagatcgc cgatgccctg ggtcgcccgg
      181 ccgagcacgc cgcggtggtt ctcgcccgcg cgctcgaccg cggccgacgc gcgcgatcct
      241 gttaggtcag aagcggccct cgacccgcac cggcggcggc gacggggtag ggcggatcgg
      301 cggcagcatg gtcggcacgt tcggccggat gtcttgaatc accccggtcg gcaggcgccg
      361 ctcgagttcc tcgcgcgcca ggccgccgat gtcgccctcg aagatgcgcc ggatgcgctc
      421 gcggcgttcg tccaggcgcg cggccatctc cgctatctgc tcggccttgc cctgggcgcg
      481 gttgacggtc tcgatcaggc tcgaccggct ggcgaccttg ccgccgtagt aggtgccggc
      541 gaggccgccg atattgcgcc cgaccttgcg cccgcggcgg ccgccgacca tgccgccgac
      601 gaacccgccg acctcgcggc cgaccttctg gccgacctcg cccggcacct gggcgacctg
      661 cgcggccgcc cgcccaatct cgccggcgcc cttgaccgtg cctttcgcca cctcgccgac
      721 gacgcggccc acgccgcgca cgacgttcct gacgccgcgg aagacttgct tcaggaacac
      781 ctcccggcgc aggtcgccga actcgcccgg atggtagtcg agcgtgagcg ggccaggacc
      841 ggccgaggcc gtcgcgcgcg gcggcgcgat ggcggcgatg tcggcgtgga ccggcggcgc
      901 gttgggtatg gccgcgacca gcagcgcgag cccgatcgcg gcgccggccg cgcagcgcgc
      961 gccgctacag ctcgtccagg atcgcttggc gcttggcacg atattcctct tcggtgatca
     1021 ggccctgatc gtggaggcgc tcgagggtct ccaggcggcg gacgagctcg gccgtgtcat
     1081 cgggcgccgc cggttcggcg gccggcgcgg ccggcgccgc cggcgccggg gcaggtgcgg
     1141 tcggctcggt cgccgcggcg ggcacgactt cgggcaggcg cggcgggccg aacggcgcga
     1201 tccggagcgg gacgctgtgg gcgtagaaca ggcggctgtc gaggaagccc agcaccagct
     1261 gctcggcgac gaccaggtcc actccggcga gacccggctc gtcgtactcg atgtagccgt
     1321 actcctcgaa ccccgccgcg ttggcggcgt cgaggaacgc catgccgctt tcgaacatgt
     1381 acgggcgatc gggcgcgtag gcgaccagcc cgctcggccg ctcgatgtcc cgggtctcgc
     1441 gcagctcggc gttgacgaag gtgaacgcct tgcccgcgcg ccaatactcc cactcctcga
     1501 cgcgcaccag ccgcgccggg tcgccggcct gggcgctgac cgcgaccgtg aacgaatccg
     1561 gcgcgcccat gcgcgacagt atgtcggcct gggcgagcag cggcttgttg tgcagcacct
     1621 ccagcgccca ccacgcggcg atccccgcct cgctgtcctc cgccgccgac acgcgggcgc
     1681 ggcggtaggc gcgctcggcg agcgagaact tgccgagcag ctcgtgcgcc aggccctggt
     1741 tgtggtggaa ggcgggatcc ttcggcgcca gccgcaccgc ctcggtgaag tcggcgagcg
     1801 ccgcctggcg gtcgccgagc tcgagccgga acaggccgcg ctggttgagc gcctcgacat
     1861 tgcccggatc ggccgcgagc gcggcgtcga gctcggtgac caggtctggg gcggcgccgc
     1921 ccgcggcctc ggcctcggtg gccacggcct cggccagctc ctgcgcctcg gcctccatca
     1981 ccagcgccac cgtttcgggg ctgggcggcc acgcggcggc ggccggcgcc gcgccaagcg
     2041 ccagcagcgc ggccagccgg ccgatcgatg ggcgggcgaa caagcgcatc gcgaccagac
     2101 tacggcccgg cggcggcgct ggcaaacgca tgccgccgct tcacgatgcg ggcaggcgct
     2161 gcaggaagcc gcccatcacc tgctccatgc tggtctggtt gaggccgagc ttggcgag
//
LOCUS       DASWHU010000205         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1968540, whole genome shotgun sequence.
ACCESSION   DASWHU010000205 DASWHU010000000
VERSION     DASWHU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968540"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGB88_07380"
     CDS             complement(<1..463)
                     /locus_tag="VGB88_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9647305.1"
                     /translation="MPSSNLTVPRPVSAARIAALDWARLGAELDGHGCAVIDGLLSGG
                     DCRGLADSYAADELFRSQVVMARHGFGRGEYKYFAYPLPDPVAALRAALYPPLAGIAN
                     RWSAAMGSDLRYPGAHGAYLERCHRDGQTRPTPLLLRYGPGDYNCLHQDLYG"
     gene            complement(573..1790)
                     /gene="glp"
                     /locus_tag="VGB88_07385"
     CDS             complement(573..1790)
                     /gene="glp"
                     /locus_tag="VGB88_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9647306.1"
                     /translation="MISVEQALESILAAFSPLPGEQVALAQALGRVLAEPVVARRTQP
                     PADMSAMDGYAVRSADTARVPATLEVVGEAPAGGSFAGTVGAGQAVRIFTGAPVPDGA
                     DAIVIQEDTEADGRRVTVKERAEAGRHVRRAGLDFRAGDIGVAAGEVLGARDLALAAA
                     MNRPWLTVRRKPRVAVLATGDEVVMPGEDIGPNQIVSSNSLALAAVIEASGGEAVMLG
                     IAPDDAEALAALAAGARGHDLLVTTGGVSVGDRDLVRRVLGERGLEVEFWRIAMRPGK
                     PLLFGSLAGTPVLGLPGNPVSALVCALVFLRPALAVMLGIDGAGAVAETTARLASPLA
                     ANDRRQDYLRARFARAADGVLEVTPFPVQDSSMLSVLAQADGLVVRPPNAPPAAAGAT
                     VPVIPFPPGLARF"
     gene            complement(1801..2277)
                     /gene="moaC"
                     /locus_tag="VGB88_07390"
     CDS             complement(1801..2277)
                     /gene="moaC"
                     /locus_tag="VGB88_07390"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX9647307.1"
                     /translation="MADFSHFDAEGKAAMVDIGAKDETERVAVARASVLMQPATLKLV
                     LSGGHSKGDVLAVARLAGIMAAKRTAELVPLCHPLALTSVAVDLACDEARDAVDITAT
                     CRLVGRTGVEMEALTAAAVAALTVYDMCKAVDRGMRITDLRLVHKSGGKSGTFDAP"
     gene            complement(2286..3101)
                     /gene="trpC"
                     /locus_tag="VGB88_07395"
     CDS             complement(2286..3101)
                     /gene="trpC"
                     /locus_tag="VGB88_07395"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9647308.1"
                     /translation="MSDALARICAAKRAEIGRRKAARPFAALVAGLAEAPPPRPFAAA
                     LDRTVAAGGAALIAEIKKASPSKGVICADFDPPALARAYQDGGAACLSVLTDGPYFQG
                     ADDHLVKARGAVGLPTLRKDFMLDVYQVLESRLLGADCILVIMAAVSDAVATELADAA
                     TATGMDALAEVHDAAELERALRLGAGLIGINNRDLKTLAVDLATTERLAPLVPAGRAV
                     VCESGIADAGDIARMRGHGVDRFLVGESLMRQADVAAATRALLAPPPARRAAG"
     gene            complement(3098..4132)
                     /gene="trpD"
                     /locus_tag="VGB88_07400"
     CDS             complement(3098..4132)
                     /gene="trpD"
                     /locus_tag="VGB88_07400"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9647309.1"
                     /translation="MDDLKPLLAKLAAGAPLSEDEAYAAFAIMMSGDATPSQTGAFLM
                     GLRVRGETVDEITGAVRAMREKAVRIKAPPGTIDTCGTGGDARGTYNVSTAAALIVAA
                     CGVPVAKHGNRAMSSRSGSADVLAALGVDIDADMAVVEAAVAEVGIGFLMAPRHHGAM
                     RHVAGTRVELGTRTIFNILGPLSNPAGAKRQLIGVFDAKWLVPMAEVLGKLGSERVWV
                     VHGSDGMDELTTTGPSAVAELAHGTVSRFEVTPEDAGLGRAKAADLKGGDAAHNAAAL
                     GAVLDGAPGAYRDIAVLNAAAALVVADKAKDLRAGARMAAAAIDGGAARDVLGRLVEI
                     TSRAHAGVGE"
     gene            complement(4140..4724)
                     /locus_tag="VGB88_07405"
     CDS             complement(4140..4724)
                     /locus_tag="VGB88_07405"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9647310.1"
                     /translation="MLVLIDNYDSFTYNLVHFLGELGADTIVHRNDAVTPEAVLAMRP
                     DAIVLSPGPCDPDRAGICLDLVAKAAGRVPILGVCLGHQAIGQAFGGRIVRAPQMMHG
                     KLSAIRHRGDGLFAGIPSPFTATRYHSLIVERSSLPDALEVTAETDDGVIMALRHREL
                     AVYGVQFHPESIASEHGHDLLANFLAVAGRRAAA"
     gene            4930..5331
                     /locus_tag="VGB88_07410"
     CDS             4930..5331
                     /locus_tag="VGB88_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEX9647311.1"
                     /translation="MDVWGHHLMLDAAGCNQRLGDGEAIKAFARELVGAIGMVAYGEP
                     IVAHFGHGDPDTCGYTLVQLIETSNITAHFCDNTGEIYLDVFSCRDFDQATVVEVFRR
                     HFAPRHINVNKIGRAAPSLAAERPLARRIIA"
     gene            5543..>5913
                     /locus_tag="VGB88_07415"
     CDS             5543..>5913
                     /locus_tag="VGB88_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647312.1"
                     /translation="MAAGAALLALGIAIGWIAGVITTAPEPTPLPAPPPVAAARPLAP
                     AEPQYDAPLPAAPRAAPGVGELETARLALPPVPAPPAARGRIALVIDDLGLLDQTTRE
                     AIALDRAVTLAFLPYGPDTAA"
BASE COUNT          788 a         2169 c         2181 g          775 t
ORIGIN      
        1 gcccgtaaag gtcttggtgc aggcagttgt agtcgcccgg cccgtaccgc agcagcaacg
       61 gcgtcggcct ggtctggccg tcccggtggc agcgctcgag atacgcgccg tgcgcgcccg
      121 ggtagcggag gtcgcttccc atggcggcgc tccagcggtt ggcgatgccg gcgagcggcg
      181 gatagagcgc ggcgcgcagg gccgcgaccg gatcgggcag gggataggcg aagtacttgt
      241 actcgccccg cccgaagccg tgccgcgcca tgaccacctg gctgcggaac agctcgtcgg
      301 cggcatagct gtcggcgagc ccgcggcagt cgccgccgct cagcaggccg tcgatcacgg
      361 cgcagccgtg gccatcgagc tccgccccga gccgcgccca gtcgagcgcg gcgatgcgcg
      421 ccgcgcttac cggccgcggg acggtcaggt ttgaggacgg catggttgcc tcatggtctc
      481 cccggcaggc gttccggtcc ttgatcgcac gacaaccgtc gctgcgctat ccgaataccg
      541 cgcgcaaact cgcgcgactt tcggcgcccg atctagaagc gggcgaggcc gggcgggaag
      601 gggatcaccg gcacggtcgc gccggcggcg gcgggcggcg cgttgggcgg gcgcaccacc
      661 aggccgtcgg cctgcgccag caccgacagc atcgagctgt cctggaccgg gaacggggtt
      721 acctccaaga cgccatccgc ggcgcgtgcg aatcttgcgc gcaggtagtc ctggcggcgg
      781 tcgttggcgg cgagcgggct cgcgagccgc gccgtggtct cggcgaccgc gccggcaccg
      841 tcgatgccga gcatcacggc aagggcgggg cgcaggaaca ccagcgcgca gaccagcgcc
      901 gacaccgggt tgccgggcag gccgagcacc ggcgtgccgg caaggctgcc gaacaacagc
      961 ggcttgcccg ggcgcatcgc gatgcgccag aactcgacct cgaggccgcg ctcgccgagc
     1021 acccggcgca ccaggtcgcg gtcgccgacc gagacgccgc cggtggtgac cagcaggtcg
     1081 tggccgcgcg cgcccgcggc cagcgccgcc agcgcctccg cgtcgtcggg ggcgatgccg
     1141 agcatcaccg cctcgccgcc cgaggcctcg atcaccgccg ccagcgccag cgagttggag
     1201 ctgacgatct ggttggggcc gatgtcctcg ccgggcatca ccacctcgtc gccggtggcc
     1261 agcacggcga cgcgcggctt gcgccggacc gtgagccagg gccggttcat cgccgccgcg
     1321 agcgcgaggt cgcgggcgcc gagcacctct ccggcggcga cgccgatgtc gccggcgcgg
     1381 aagtcgagcc cggcgcgacg gacgtggcgt ccggcctcgg cgcgctcctt gaccgtgacc
     1441 cgccggccgt cggcctcggt gtcctcctgg atgacgatcg cgtcggcgcc gtcgggcacc
     1501 ggcgcgccgg tgaagatgcg caccgcctgg ccggccccga cggtgccggc gaagctgccg
     1561 ccggccggcg cctcgccgac cacctccagg gtggccggca cgcgcgcggt gtcggcgctg
     1621 cgcacggcgt agccgtccat cgccgacatg tcggccggcg gctgggtgcg gcgcgcgacg
     1681 accggctcgg ccaggacgcg cccgagcgcc tgcgccagcg ccacctgctc tccgggcaac
     1741 ggggagaacg cggcgaggat cgactcgagc gcctgctcga ccgagatcat gggcgcaccg
     1801 tcaaggcgcg tcgaaggtgc ccgacttgcc gccggacttg tggaccaggc gcaggtcggt
     1861 gatgcgcatg ccgcggtcga ccgccttgca catgtcgtag accgtgagcg cggcaaccgc
     1921 cgcggcggtg agcgcctcca tctccacccc ggtgcgcccg accaggcggc aggtggcggt
     1981 aatgtcgacc gcatcgcgcg cctcgtcgca ggcgaggtcg accgcgaccg aggtcagcgc
     2041 cagcggatgg cacagcggga ccagctcggc ggtgcgcttg gcggccatga tgccggcgag
     2101 acgggcgacg gcgagcacgt cgcctttgct gtggccgccg ctcaggacca gcttgagcgt
     2161 cgccggctgc atcagcacgc tggcgcgcgc caccgccacg cgctcagtct cgtccttggc
     2221 gccgatatcg accatcgcgg ccttgccctc ggcatcgaag tggctgaagt cggccacggt
     2281 gccaatcagc cggcggcgcg gcgcgccggc ggcggcgcca gcagcgcgcg cgtcgcggcg
     2341 gcgacgtcgg cttggcgcat cagcgactcg ccgaccagga accggtcgac gccgtggccg
     2401 cgcatgcgcg cgatgtcgcc cgcatccgca atgccgctct cgcacaccac ggcgcggccg
     2461 gcgggaacca gcggcgccag ccgctcggtg gtcgctaagt cgaccgccag cgtcttgagg
     2521 tcgcggttgt tgatgccgat caggccggcg ccgagcctga gcgcgcgttc gagctcggcg
     2581 gcgtcgtgga cctcggcgag ggcgtccatg ccggtcgcgg tcgcggcgtc ggcaagctcc
     2641 gtcgccaccg cgtcgcttac cgcggccatg atcaccagga tgcagtcggc gccgagcagg
     2701 cgggactcga gcacctggta gacgtcgagc atgaagtcct tgcggagtgt cggcaggccg
     2761 accgcgccgc gcgccttgac caggtggtcg tcggcgccct ggaaataggg gccgtcagtc
     2821 agcaccgaca ggcaggccgc gccgccgtcc tgataggcgc gggcgagcgc cggcgggtcg
     2881 aagtcggcgc aaatcacgcc cttgctcggc gacgccttct tgatctcggc gatcaaggcg
     2941 gcgccgccgg cggcgacggt gcggtcgagg gcggcggcga acgggcgcgg gggcggtgcc
     3001 tccgcgagcc ccgccaccag cgccgcgaac ggccgcgccg ccttgcgccg gccgatctcc
     3061 gcgcgcttgg cggcgcagat cctcgccagg gcgtcgctca ttcgccgacc ccggcgtggg
     3121 cgcggctggt gatctcgacc aggcgcccga gcacgtcccg cgccgcgccg ccgtcgatcg
     3181 ccgccgccgc catccgggcg ccggcgcgca ggtccttggc cttgtccgcg accaccagcg
     3241 cggcggcggc gttgagcacg gcaatgtcgc gataggcgcc gggcgcgccg tcaagcacgg
     3301 cacccagcgc ggcggcgttg tgcgcggcgt cgccgccttt caggtccgcg gctttggcgc
     3361 ggccgagccc ggcatcctcg ggcgtcacct cgaaccgcga caccgtgcca tgggcgagct
     3421 cggcgaccgc ggacgggccg gtcgtggtca gctcgtccat gccgtccgag ccgtggacca
     3481 cccagacgcg ctcggagccg agcttgccca gcacctcggc catcggcacc agccacttgg
     3541 cgtcgaacac gccgatcagc tggcgcttgg cgccggccgg gttcgacagc ggccccagga
     3601 tgttgaagat ggtgcgggtg ccgagctcga cccgggtgcc ggcgacgtgg cgcatggcgc
     3661 cgtgatggcg cggcgccatc aggaagccga tgccgacctc ggcgaccgcc gcctcgacca
     3721 ccgccatgtc ggcgtcgatg tcgacgccga gggccgcgag cacgtcggcg gagcccgagc
     3781 gcgaggacat cgcccgattg ccgtgcttgg ccaccggcac gccgcaggcg gcgacgatca
     3841 gtgccgcggc ggtcgagacg ttgtaagtgc cgcgcgcgtc gccgccggtg ccgcaggtgt
     3901 cgatggtccc cggcggcgcc ttgatgcgca ccgccttctc gcgcatcgcc cgcaccgcgc
     3961 cggtgatctc gtcgaccgtc tcgccgcgca cccgcagccc catcaggaag gcgccggtct
     4021 gcgacggcgt cgcgtcgccc gacatcatga tcgcgaacgc ggcgtaggcc tcgtcctcag
     4081 acagcggcgc gccggcagcc agcttggcga gcaggggctt gaggtcgtcc atcgcgccct
     4141 cacgccgccg cgcgacggcc ggcgacggcg aggaaattgg cgagcagatc gtggccgtgc
     4201 tcggaggcga tgctctcggg atggaactgg actccgtaga ccgcgagctc gcggtggcgg
     4261 agcgccatga tcaccccgtc gtcggtttcc gcggtgacct cgagcgcatc gggcaagctc
     4321 gagcgctcga cgatcagcga atggtagcgg gtcgccgtga acggcgaggg gatgccggcg
     4381 aacaggccgt cgccgcggtg gcggatcgcg ctcagcttgc cgtgcatcat ctgcggcgcg
     4441 cgcacgatgc ggccgccgaa cgcctggccg atcgcctggt ggccgaggca cacgcccaaa
     4501 atcggcaccc gtcccgccgc cttggcgacc aggtcgaggc agatgccggc acggtcggga
     4561 tcgcacggcc ccggcgacag cacgatcgcg tcggggcgca tggccagcac cgcctcgggc
     4621 gtgaccgcgt cgttacgatg cacgatcgta tcggcgccca gctcgcccag gaagtggacc
     4681 aggttgtagg taaagctgtc gtagttgtcg atcaggacca gcatcgtcgc acgcttcgcc
     4741 gcggcgccca tcaggccctg ccagcagggc gtcggatcgg ccaacaaacc agcccgtcca
     4801 ggcctcgtca agagcgccgc ccgagcggcg attgagcaag gctgtctaaa gtctcgacac
     4861 gacctttggg atcgtatatg agcggcgcct gcatcaagcc gggcggcgat ctggccggga
     4921 gctttgccaa tggacgtgtg gggccatcat ctgatgctcg atgccgccgg ctgcaaccag
     4981 cgcctgggcg acggcgaggc gatcaaggcg ttcgcgcgcg agctggtcgg ggcgatcggc
     5041 atggtcgcct acggcgagcc gatcgtggcc catttcggcc acggcgatcc tgacacctgc
     5101 ggctacacgc tggtccagct gatcgagacc tcgaacatca ccgcccattt ctgcgacaac
     5161 accggcgaga tctatctcga cgtgttctcg tgccgtgact tcgaccaggc gaccgtggtc
     5221 gaggtgttcc ggcgccactt cgcgccgcgg cacatcaacg tcaacaagat cggccgcgcc
     5281 gccccgtcat tggcggcgga acggccgctg gcgaggcgca tcatcgcgtg acgcgcgccg
     5341 ccgcgctcgc ggcggccact tgccgtgcgc taacatcgcc gcgtgacctc gcggattcga
     5401 gccttcggca gcggaccttg gccaggggcg ggcgcgccgc accgttcggc gggtgagcgc
     5461 gcgccgtagg cgcggggcga tgcgcggcgt ccgggcgcgc gcgcgtctgg tccggaggca
     5521 ggcgcgtcgg gcgactcgcc tggtggcggc aggggcggcg ctgctcgctc tcggcatcgc
     5581 catcggctgg attgccggcg tcatcaccac cgcgcccgaa ccgacgccgc tgcccgcgcc
     5641 tccgccggtc gcggcggcgc ggcctttagc gccggccgag ccccaatatg acgccccgtt
     5701 gccggcggcg ccgcgcgccg cgccgggcgt cggcgagctc gagaccgcgc gcctcgcgct
     5761 gccgccggtg ccggcgccgc cggccgcgcg cggccggatc gcgctggtca tcgacgatct
     5821 cggtctgctc gaccagacca cccgtgaggc gatcgctctc gaccgggcgg tgactctcgc
     5881 cttcctgccc tacgggccgg acaccgcggc cta
//
LOCUS       DASWHU010000206         9192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3417447, whole genome shotgun sequence.
ACCESSION   DASWHU010000206 DASWHU010000000
VERSION     DASWHU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3417447"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VGB88_07420"
     CDS             complement(<1..1008)
                     /locus_tag="VGB88_07420"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9647313.1"
                     /translation="MSQPTPTDARAAEVPEPRSRFAVASWCLFDWANSPYPTVVITFV
                     FAVYFQRAVVGEAVRATALWGQAIAVSALAVALIAPVLGAAADAGARRKPWLGACSGL
                     MAATCAGLWYATPEPSSVWLVLALVALGNFAFELSMVFYNAMLPGLAGPGRIGRLSGW
                     AWALGYAGGLACLAVALIGFVQAEPPPFGLDAERAEPVRAVALLVALWFAAFGWPLFA
                     FTPDLAGARLSLGRSVAVGAATLWATLRAARSYGNIVRFLIARMLYIDGLNTLFAFGG
                     LYAAGSFAMSMVEVLEFGIALNVTAALGAFALAWVDDWLGAKRTLILSLLALIAAGSA
                     IL"
     gene            1193..1399
                     /locus_tag="VGB88_07425"
     CDS             1193..1399
                     /locus_tag="VGB88_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9647314.1"
                     /translation="MAVVKVSPKFQVVIPREIREALGIEPGQQVQALQYLDRIEYIPL
                     KRAKAMRGFLRGIDTNSLRDEDRA"
     gene            complement(1534..6357)
                     /locus_tag="VGB88_07430"
     CDS             complement(1534..6357)
                     /locus_tag="VGB88_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX9647315.1"
                     /translation="MASERRAADLERVEQVIELARARSEGARAREIELFLRQYYRRAA
                     PDDLAGRSAEDLYFSALALLNFMREHKPGSDRIRVYTPDLDADGWSSSHTVIDVVTDD
                     RPFLVDSLSAELHLREIPVHLVLHPIFHVRRDDRGNLVELFARGQAPDDARAESVIRF
                     EVGRQAEPEGTGALLARLRAILRDARLAVEDWRAILAALDRAVDALQASAPPPRTDLD
                     ETVAFLRWLADNNFTFLGYRAYDLVGARHQGRLKAVADSALGIFRSPDYDAKGGAPAR
                     GRPVTPEVMARLTDAALMLITKSARRSSIHRPTHMDSIEVKRYAEDGSVAGVHRFLGL
                     FTAAAYHQSPIAIPLLRQKIAIAIARAGFLAHSHDGKALQNVLETFPRDELFQITDDE
                     LLETSMGILRLEERPRLRLFSRRDSFGWSVSCLVFVPRDRFDSRLRRRITQVLEQAYD
                     GSCQAFYTQLGDDALARLHVIIKTTPGKIPDISAEAVEARLVAILHGWEDNLHDALFA
                     AHDERRAALIERRYAGAFPSGYRDRFAARAAVLDIERIEEALAGDGLALNLYRPPEAV
                     DEGVRFKIYHRGGPLALSDVLPMLENMGLRVIDEFPYRLQLGDGAPEAWIHDFGVVDR
                     AHRTIDVAETKERFHDAFARIWRGEVENDGFNQLITRAGLGWRQVMVLRAYSKFLRQA
                     RIPFSQAYMQRTLAQKPRFARLLVALFEERFDPGLGRAHDEDRIVADITAALDEVDSL
                     DEDRIIRRFLNAVQATLRTNYYQREAGGDPHPYLSFKLDSARLDDLPRPRPWVEIFVY
                     GARMEGCHLRGGKVARGGLRWSDRPEDFRTEVLGLMKAQMVKNAVIVPVGAKGGFVVR
                     RPPRGDREALLAEGIACYQTLVRGLLDVTDNLVAGKLVPPADVVRYDGDDPYLVVAAD
                     KGTASFSDIANQVSADYGFWLGDAFASGGSAGYDHKKIGITARGAWESVKRHFRELGR
                     DIQNQPFTVAGIGDMSGDVFGNGMLQSRQIRLVAAFNHLHVFVDPDPDPEVSYAERKR
                     LFETPRTSWGDYDAKLISPGGAVFERKAKSVSLSPAARAALGIERERMTPNELIAAIL
                     SAPVELLWNGGIGTYVKAREESHADAGDRANDAVRVDATQLRCRVVGEGGNLGFTQRA
                     RIEAARAGVRLNTDAIDNSAGVDCSDHEVNIKVLLGDVEGEGELTRKQRDKLLVQMTD
                     EVAAQCLNDNYLQTLGISVIESLGPARLDLQQRMMQTLERAGRLDRAVEFLPGDEQLD
                     ELRNQGRGLTRPEISVLYAYAKIALFGELLQSDLPDDPYLASELDLYFPEPLIKKYRK
                     FIRRHRLRREIIATRLANSIVNRASVVFALMAQEETGRDASDVARAYVITRDAFSLRA
                     LWAEIESLDNKLPAALQTEMILALRGLMEHSSLWFLRNRPQPLDCGPIIEQFSGGLAA
                     LTAGLEDALPEDRRQAVRARVEELARQGVPESLARVIGGVDPLYSSCNIIEAAMRCRL
                     AVPECAAVYYHLGSRLGLDWLRAQARRIAADNHWWRQAVDAIIDDLYGQQMALTIRVV
                     ESAGAGADAVERWAGDNQAIMARNAQLFADLRAQAGFDLAMLAVANRQTRDLIKA"
     gene            6585..7493
                     /locus_tag="VGB88_07435"
     CDS             6585..7493
                     /locus_tag="VGB88_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9647316.1"
                     /translation="MSRNPSLAAVLALVVAAASAAAAAPTPLVDAAWLARHGCDAGVV
                     VLDIRDNARAFERARVPCAVHAPYAGLGWREERDGVPGMLPAPAALAAMIGGLGVGDA
                     DHVVVVGEGGGALDMTAATRVYWTFKVLGHDAVSLLDGGFAAYRAARDLALDKGETPA
                     PAAKAFSARLQPELVASAADIAAGGVALVDARESDYFVGVNRSASAGRAGTIPGADNV
                     PISWLTTPDGRFQDRGLLAAIAAATALTSDRPLIAFCNTGQMASLDWFVSHELLGNDA
                     ARLYDGSMAEWSRDAARPVERRIGGD"
     gene            7636..9189
                     /locus_tag="VGB88_07440"
     CDS             7636..9189
                     /locus_tag="VGB88_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9647317.1"
                     /translation="MLGDILTAKGLVSRADVERAFERQKQAGGRLGDNLVALGLVTKA
                     QIDSVLNEAPPSPRSLADTGISAADLFRLMIKTIYAENHESPSTVSESLKLPYPVISQ
                     LLKEAAARKLVEPLSSAGGNGGAATEIRFALTGAGRSFAIDALEQSQYTGTAPVSLAA
                     FAERVQLQKVTNERIDRDTIEDHFADLVVEQEFIRQIGPAINSGRTVLLYGPPGNGKT
                     SVAERVAKMFSDVVYIPYAVEIEGQIMKVFDGSVHHPVERETNGQGPTLRREPIDRRW
                     VPCERPIVVTGGELTLDMLDLQYNQSARFYEAPMHVKALNGTFIIDDFGRQLVAPEDL
                     LNRWIVPLESRVDYLKLHTGKSFQFPFDELVIFSTNLSPADLMDAAFLRRIPYKLSTV
                     APDRDQFAKIFRAVAGARKLEVTEEEVDVVIDFLREQNDFPLACYQPKFIVEQVTDAC
                     KYEGVAPGLTPELVAQALSNLYTTDSPGFGITRAMRDAGHAGAGDAYTKTAAKGGGAA
                     EKVKAAALT"
BASE COUNT         1370 a         3239 c         3281 g         1302 t
ORIGIN      
        1 caggatcgcg ctgccggccg cgatcagggc gagcagcgac aggatcaagg tgcgcttggc
       61 gccgagccag tcgtcgaccc aggcgagcgc gaacgcgccg agcgcggcgg tgacgttgag
      121 ggcgatgccg aactcgagca cctcgaccat cgacatggcg aagctgccgg ccgcgtacag
      181 cccgccgaac gcgaacaagg tgttgaggcc gtcgatgtag agcatgcgcg cgatcaggaa
      241 gcggacgatg ttgccgtagc tccgcgccgc ccgcagcgtc gcccacaggg tggcggcgcc
      301 gaccgcgacc gaccgcccga gcgacagccg cgcgccggcg aggtcggggg tgaacgcgaa
      361 cagcggccag ccgaaggcgg cgaaccacag cgccaccagc agcgccaccg cgcgcaccgg
      421 ctcggccctc tcggcatcga gcccgaacgg cggcggctcg gcctggacga agccgatcag
      481 cgcgacggcg aggcaggcaa gcccgccggc atagccgagc gcccacgccc agcccgacag
      541 ccgcccgatc cgccccggcc ccgcgagccc cggcagcatg gcgttgtaga acaccatgct
      601 gagctcgaac gcaaagttgc cgagagccac cagcgccagc accagccaca ccgaggacgg
      661 ctccggcgtc gcgtaccaca gcccggcgca ggtcgcggcc atcaggcccg agcaggcgcc
      721 gagccacggc ttgcggcgcg cgccggcgtc ggccgcggcg ccgaggaccg gcgcgatcag
      781 cgccaccgcc agcgcgctca ccgcgatcgc ctggccccac agcgcggtcg cgcgcaccgc
      841 ctcgccgacc accgcgcgct ggaagtagac cgcgaacacg aaggtgatga cgaccgtcgg
      901 atagggcgag ttggcccagt cgaacaggca ccagctcgcc accgcgaatc gcgagcgtgg
      961 ctcaggcacc tcggcggcgc gcgcgtccgt cggcgtaggc tgggacatcg gcggagccgc
     1021 cctcgggcga tcaggcctcg accgcggccg aggctacgcg aagcgcaaca caccgtccac
     1081 ggcgctcgcc ggcccacgca ccagcgctcg caaataactt ccgtgcccgg cctgcaaact
     1141 gacgtataac gcgcacctca tggacgcgac caacgcaaag acggagcagg cgatggctgt
     1201 tgtcaaggtg tcacccaagt tccaggtggt gatcccccgc gaaattcgcg aggcgttggg
     1261 gatcgaacct ggccagcagg tccaggcgct gcaatatctc gaccgcatcg agtacatccc
     1321 gctcaagcgg gcgaaagcca tgcgcggctt tttgcgagga atcgacacca actcgctgcg
     1381 cgacgaggat cgtgcatgaa cacgaacgag acgtcatcgc gagccgccgg aggcggcgtg
     1441 gcgatccaga catagaccgc aagaccgagc cggctggatt gcttcgcccg cttttcgggc
     1501 tcgcaatgac aacttttcaa ctcctggcac ggctcacgcc ttgatcaggt cgcgggtctg
     1561 gcggttggcg accgcgagca tggcgaggtc gaagcccgcc tgcgcgcgca ggtcggcgaa
     1621 cagctgggcg ttgcgggcca tgatcgcctg gttgtcgccg gcccagcgct cgaccgcgtc
     1681 ggcgcccgcg ccggcgcttt cgaccacccg gatggtgagc gccatctgct ggccgtagag
     1741 gtcgtcgatg atcgcgtcca ccgcctggcg ccaccagtga ttgtcggcgg cgatccgccg
     1801 cgcctgggcg cgtagccagt cgaggccgag ccggctgccg agatggtagt agaccgccgc
     1861 gcactcgggc accgcgagcc ggcagcgcat cgccgcctcg atgatgttgc agctcgaata
     1921 caagggatcg acgccgccga tcacgcgcgc caggctttcg ggcacgccct ggcgcgcgag
     1981 ctcctcgacc cgggcgcgca ccgcctggcg gcggtcctcg ggcagcgcgt cctcaagccc
     2041 ggcggtgagc gcggcgaggc cgcccgagaa ctgctcgatg atcggcccgc agtcgagcgg
     2101 ctgcgggcgg ttgcgcagga accacaggct cgaatgctcc atcagcccgc gcaaggcgag
     2161 gatcatctcg gtctgcaacg ccgccggcag cttgttgtcg agcgactcga tctccgccca
     2221 cagcgcgcgc aaggagaacg catcgcgggt gatcacgtag gcgcgggcga cgtcgctggc
     2281 gtcgcgcccg gtctcctcct gcgccatcag cgcgaacacg acgctggcgc ggttgacgat
     2341 gctgttggcg agccgggtgg caatgatctc gcgccgcagg cgatggcggc ggatgaactt
     2401 gcggtacttc ttgatcagcg gctcggggaa gtagaggtcg agctcgctcg ccagatacgg
     2461 gtcgtccggc aggtccgact gcagcagctc gccgaacagc gcgatcttgg cgtaggcgta
     2521 cagcactgag atctcgggcc gggtcaggcc gcgcccctga ttgcgcagct cgtcgagctg
     2581 ctcgtcgccg ggcaggaact cgaccgcgcg gtcgaggcgc ccggcgcgct cgagcgtctg
     2641 catcatgcgc tgctgcaagt ccaggcgcgc cggcccgagc gattcgatca cgctgatgcc
     2701 gagcgtctgc aagtagttgt cgttgaggca ttgggcggcg acttcgtcgg tcatctgcac
     2761 cagcagcttg tcgcgctgct tgcgcgtcag ctcgccctcg ccctcgacgt cgccgagcag
     2821 gaccttgatg ttgacctcgt ggtcggagca gtcgacgccg gccgagttgt cgatggcgtc
     2881 ggtgttgagg cgcacgcccg cgcgcgcggc ctcgatgcgc gcgcgctggg tgaagccgag
     2941 attgccgccc tcgccgacca cccggcagcg cagctgggtg gcgtcgacgc gcaccgcgtc
     3001 gttggcgcgg tcgccggcgt cggcgtgcga ctcctcgcgc gccttgacgt aggtgccgat
     3061 gccgccgttc cacaacagct cgaccggcgc gctcaagatc gccgcgatca gctcgttggg
     3121 ggtcatgcgc tcgcgctcga tgccgagtgc cgcccgcgcc gcggggctca agctgaccga
     3181 cttcgccttg cgctcgaaca ccgcgccgcc gggcgagatc agcttggcgt cgtagtcgcc
     3241 ccagctcgtg cgcggggtct cgaacaggcg cttgcgctcg gcgtagctca cctcggggtc
     3301 ggggtccggg tcgacgaaca catgcaggtg gttgaacgcc gcgaccagcc tgatctggcg
     3361 cgactgcagc atgccgttgc cgaacacgtc gcccgacatg tcgccgatgc cggcgacggt
     3421 gaacggctgg ttctggatgt cgcggccgag ctcgcggaag tgccgcttga ccgattccca
     3481 ggcgccgcgc gcggtgatcc cgatcttctt gtggtcgtag ccggccgagc cgccgctggc
     3541 gaaggcgtcg cccagccaga agccgtagtc ggcggaaacc tggttggcga tgtccgagaa
     3601 gctcgccgtg cccttgtcgg cggcgaccac cagatagggg tcgtcgccgt cgtagcggac
     3661 cacgtcggcg ggcggcacca gcttgccggc gaccaggttg tcggtgacgt cgagcaggcc
     3721 gcgcaccagg gtctggtagc aggcgatgcc ctcggcgagc agcgcctcgc ggtcgccgcg
     3781 cggcggccgc ctgaccacga acccgccctt ggcgccgacc ggcacgatga ccgcgttctt
     3841 gaccatctgc gccttcatca ggccgagcac ctcggtgcgg aagtcctcgg gccggtccga
     3901 ccagcgcagg ccgccgcggg cgaccttgcc gccgcgcaga tggcagccct ccatgcgcgc
     3961 gccgtagacg aagatctcga cccagggccg gggccggggc aggtcgtcga ggcgcgcgga
     4021 gtcgagcttg aacgacaggt aggggtgcgg atcgccgccg gcctcgcgct ggtagtagtt
     4081 ggtccgaagc gtcgcctgca cggcattgag gaagcggcgg atgatgcggt cctcgtcgag
     4141 gctgtcgacc tcgtcgagcg cggcggtgat gtcggcgacg atcctgtcct cgtcgtgcgc
     4201 gcgcccgagg ccggggtcga agcgctcctc gaacagggcg accagcagcc tggcgaagcg
     4261 cggcttctgg gccagcgtgc gctgcatgta ggcctggctg aacgggatcc tggcctggcg
     4321 caggaacttg ctgtaggcgc gcagcaccat cacctggcgc cagccgagcc cggcgcgggt
     4381 gatcagctgg ttgaagccgt cgttctcgac ctcgccccgc cagatgcgcg cgaacgcgtc
     4441 gtggaaccgc tccttggtct cggcgacgtc gatcgtccgg tgggcccggt cgacgacgcc
     4501 gaagtcgtgg atccaggcct cgggcgcgcc gtcgccgagc tgcagccggt aggggaactc
     4561 gtcgatcacg cgcaggccca tgttctcgag catcggcagc acgtccgaca gcgccagcgg
     4621 cccgccgcgg tggtagatct tgaaccggac cccttcgtcg accgcctcgg gcgggcgata
     4681 caggttgagc gccaggccgt cgccggcgag cgcttcctcg atccgctcga tgtcgagcac
     4741 cgcggcgcgg gcggcgaagc ggtcgcgata gccggacggg aaggcgccgg catagcgccg
     4801 ctcgatcagc gccgcgcgcc gctcgtcgtg ggccgcgaac agcgcgtcgt ggaggttgtc
     4861 ctcccagccg tgcaggatcg ccaccaggcg cgcctcgacc gcctcggccg agatgtccgg
     4921 gatcttgccc ggcgtggtct tgatgatcac gtgcaggcgc gccagcgcgt cgtcgccgag
     4981 ctgggtgtag aacgcctggc agctgccgtc gtacgcctgc tcgaggacct gggtgatgcg
     5041 ccggcgcagg cgcgagtcga agcggtcgcg gggcacgaac accaggcacg acaccgacca
     5101 gccgaagctg tcgcggcggc tgaacaggcg cagcctgggg cgctcctcca ggcgcaggat
     5161 gcccatgctg gtctcgagca gctcgtcgtc ggtgatctgg aacagctcgt cgcgcgggaa
     5221 ggtctcgagc acgttctgca acgccttgcc gtcgtggctg tgggccagga acccggcgcg
     5281 cgcgatggcg atcgcgatct tctggcgtag cagcggaatc gcgatcggcg actggtgata
     5341 ggcggcggcg gtaaacaggc cgaggaagcg gtgcacgccg gcgaccgagc cgtcctcggc
     5401 gtagcgcttg acctcgatcg aatccatgtg ggtcggacgg tggatgctgg accggcgcgc
     5461 gctcttggtg atcagcatca gcgcggcgtc ggtcagccgc gccatcacct ccggcgtcac
     5521 cggccgcccg cgcgcgggcg cgccgccctt ggcgtcgtag tccgggctgc ggaagatgcc
     5581 gagcgcgctg tcggcgaccg ccttcagccg tccctggtgc ctggcgccga ccaggtcgta
     5641 ggcgcggtag ccgaggaagg tgaagttgtt gtcggccagc cagcgcagga aggcgaccgt
     5701 ctcgtcgagg tcggtgcgcg gcggcggcgc gctcgcctgc agcgcatcca ccgcccggtc
     5761 gagggcggcc agtatcgccc gccagtcctc gactgcgagc cgcgcgtcgc gcaggattgc
     5821 gcgcaggcgc gcgagcagcg cgccggtgcc ctcgggctcc gcctggcggc cgacctcgaa
     5881 ccggatcacc gattcggcgc gcgcgtcgtc cggcgcctgg ccgcgggcga acagctcgac
     5941 caggttgccc cggtcgtcgc ggcgcacatg gaagatcggg tgcagcacca ggtggaccgg
     6001 gatctcgcgc aggtgcagct cggcggacag cgaatcgacc aggaacgggc gatcgtcggt
     6061 gaccacgtcg atgacggtgt gcgacgacga ccagccgtcg gcatcgagat cgggggtgta
     6121 gacccggatg cggtcgctgc cgggcttgtg ctcgcgcatg aagttgagga gcgcgagggc
     6181 ggagaagtag aggtcctcgg cgctgcgccc ggcaaggtcg tcgggcgccg cccggcggta
     6241 gtactggcgc aggaacagct cgatctctcg cgcccgggcg ccctcgctgc gggcgcgcgc
     6301 aagctcgatc acctgctcaa cccgctccag gtccgcggcg cgtctctccg aggccatggc
     6361 cgaccctctg ctggctcgcc ccgcccctgc ggcgctgcgc gccgccgggg ccgccggggg
     6421 tttcgttgcc tgatcgaata ctgttcgaat tagggccgcg cgagcgtact gcaatcgccg
     6481 gccgcgggcc atgcgcagcc gcggccgcgg cgccggcggc tttgcggcac gacgcgcaag
     6541 gggctatcgt gcgcgctcga cccgttgccg ctgggaggcc cgcaatgtcg cgcaatccgt
     6601 ctctggccgc cgtcctcgcg ctcgtcgtcg ccgccgcaag cgcggcggcg gcggcgccga
     6661 cgcccctggt cgatgccgcc tggctcgccc ggcatggctg cgatgccggc gtggtggtgc
     6721 tcgacatccg cgacaatgcg cgcgccttcg agcgcgcccg ggtgccgtgt gcggtgcacg
     6781 cgccctatgc gggcctcggc tggcgcgagg agcgcgacgg cgtgcccggc atgctgcccg
     6841 cgcccgcggc gctggcggcg atgatcggcg gcctcggcgt cggcgatgcg gaccatgtgg
     6901 tggtagtggg cgagggcggc ggcgcgctcg acatgaccgc ggccacccgg gtctactgga
     6961 cgttcaaggt gctcggccac gacgcggtct cgctgctcga tggcggcttc gccgcctacc
     7021 gcgcggcgcg cgatctggcg ctcgacaagg gcgagacgcc ggcgccggcg gcgaaggcct
     7081 tcagcgcccg cctgcagccc gagctggtgg cgagcgccgc cgacatcgcg gccggcggcg
     7141 tggcgctggt ggatgcccgc gaatccgact acttcgtggg cgtcaaccgc tccgccagtg
     7201 ccgggcgcgc gggcaccatt ccgggcgccg acaatgtgcc gatctcgtgg ctgaccaccc
     7261 cggacggccg gttccaggac cgcgggctgc tcgcggccat tgcggccgcc accgcgctca
     7321 cgtcagaccg gccgctgatc gccttctgca ataccggcca gatggcctcg ctcgactggt
     7381 tcgtcagcca cgagctgttg ggcaacgacg ccgcgcgcct ctatgacggc tcgatggcgg
     7441 agtggagccg cgacgccgcc cgcccggtcg agcgccggat cggcggcgac tgaccccgac
     7501 ccggcccccg tccgggcgtc aatcggtcct gggcgggggc gcccatcggc ctaacataat
     7561 ctaaacaatt tgcgagcttg cggcgtcgcc gccggcgccc gccgcggccg acccgacgga
     7621 ttgggagatc ggattatgct tggcgacatt ttaactgcca agggcctggt ttcgcgcgcc
     7681 gacgtggagc gcgcgttcga gcgtcagaag caggccggcg gccggctggg cgacaacctg
     7741 gtcgcgcttg gcctggtgac caaggcccag atcgactcgg tgctcaacga ggcgccgccc
     7801 tcgccgcgct cgctggccga taccggcatt tccgccgccg acctgtttcg gttgatgatc
     7861 aagaccatct acgccgagaa ccacgagtcg ccctcgacgg tcagcgagtc gctgaagctg
     7921 ccctacccgg tgatcagcca gctgctgaag gaggccgccg cgcgcaagct ggtcgagccg
     7981 ctgagcagcg ccggcggcaa cggcggcgcg gcgaccgaga tccgcttcgc gctgaccggc
     8041 gccgggcgct cgttcgccat cgatgcgctc gagcagagcc agtacaccgg caccgcgccg
     8101 gtttcgctcg cggccttcgc cgagcgcgtc cagctgcaga aggtgaccaa cgagcgcatc
     8161 gaccgcgaca ccatcgagga ccacttcgcc gacctggtcg tcgagcagga gttcatccgc
     8221 cagatcggcc cggcgatcaa ctcggggcgc acggtgctgc tctacggccc gcccggcaac
     8281 ggcaagacct cggtcgccga gcgcgtcgcc aagatgttca gcgacgtcgt ctacatcccc
     8341 tacgcggtcg agatcgaagg ccagatcatg aaggtgttcg acggcagcgt gcaccatccg
     8401 gtcgagcgcg agaccaacgg ccaggggccc accctcaggc gcgagccgat cgaccggcgc
     8461 tgggtgccgt gcgagcgccc gatcgtggtc accggcggcg agctgaccct cgacatgctc
     8521 gacctccagt acaaccagtc ggccaggttc tacgaggcgc cgatgcacgt gaaggcgctc
     8581 aacggcacct tcatcatcga cgacttcggc cgccagctgg tggcgcccga ggacctcttg
     8641 aaccgctgga tcgtgccgct cgagagccgg gtcgattacc tcaagctgca caccggcaag
     8701 agcttccagt tcccgttcga cgagctggtc atcttctcga ccaacctgtc gccggcggac
     8761 ctgatggacg cggcgttcct gcgccggatt ccctacaagc tgtcgaccgt ggcgcccgac
     8821 cgcgaccagt tcgccaagat cttccgcgcg gtggccgggg cgcgcaagct ggaggtgacc
     8881 gaggaggagg tcgacgtggt gatcgacttc ctgcgcgagc agaacgactt cccgctcgcc
     8941 tgctaccagc ccaagttcat cgtcgagcag gtgaccgacg cgtgcaagta cgagggcgtc
     9001 gcgcccgggc tgacgcccga gctggtcgcc caggcgctga gcaacctgta caccaccgat
     9061 tcgcccggct tcggcatcac ccgcgcgatg cgcgacgccg gccacgccgg cgccggcgac
     9121 gcctatacga agaccgcggc caagggcggc ggcgccgccg agaaggtcaa ggccgccgcg
     9181 cttacctgag cc
//
LOCUS       DASWHU010000207        28642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_477426, whole genome shotgun sequence.
ACCESSION   DASWHU010000207 DASWHU010000000
VERSION     DASWHU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 28642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28642
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477426"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGB88_07445"
     CDS             <1..206
                     /locus_tag="VGB88_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647318.1"
                     /translation="RRATGAYVAFTVAMVLVMIFPVYGLGNRVEPFVLGLPWSLAWVV
                     FWIGVEFVGLIAFYLYEHGGEGG"
     gene            211..1674
                     /locus_tag="VGB88_07450"
     CDS             211..1674
                     /locus_tag="VGB88_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9647319.1"
                     /translation="MAQWVVALGAMSAYLAFAFALGILAGRNRSFFSVSEYAVADRGL
                     GLVVMWFLMGGTIFSAFAFLGGPGWAYEKGAASFYILAYASLGLLPWYLIGPKVARLG
                     RAGNYFTMGDLLADRYRSRALVVLVGVVSVLAFIQYLTLQIKGMAYVFSVLTEGAVPF
                     WLGALLAYGIVVVYVATSGVRGAAWSDVLQGILMLAVAWVLGLWLVTTLHGGLGPMFA
                     AIATERPGLLVIGAEGSAMSATAYSSAIVVSTLGFMMWPHLFMKSYTTSERRIKLTVL
                     AYPVFAIFLVPVLLIGFAAIGVVAPESLASTDQVLPHLITYEIGASGLVYGLIGAGAL
                     AAAMSSSDAITHGASVSVGRDILVPLNPAVAERTQLWVMRAAVVGVGAIAYYLAIFGA
                     EGLIQLLLGAYGSIVQFAPAVYGALYWRRGTGAGALAGLAAGVAVNFTFQLGAPAPPL
                     DIHPGLLGLAVNLAVFAAVSLLTRPAERSLVVRFVDA"
     gene            1733..2254
                     /gene="tsaE"
                     /locus_tag="VGB88_07455"
     CDS             1733..2254
                     /gene="tsaE"
                     /locus_tag="VGB88_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9647320.1"
                     /translation="MQTKARTDPGTNEAIEITLADEAATRRLAAAVAAVARASDVIAL
                     SGPLGAGKTSFARGFVDALGATEEVPSPTFTLVQTYALAPAAVWHFDLYRLERPTDAY
                     ELGIEDAFDAGISLIEWPARLGALLPADRLDVALDFAAGEGTGRIGRLIGHGAWHARL
                     AAVMASWAAAGDG"
     gene            2247..3251
                     /locus_tag="VGB88_07460"
     CDS             2247..3251
                     /locus_tag="VGB88_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9647321.1"
                     /translation="MAEAGARAALIERFLAAHGWAGGRRTPLAGDASFRRYQRLRRGL
                     RRAVLMDAPPGHEDVRPYCRIARHLTGLGYSAPAIYAEDADAGLLLIEDLGDDTYSRL
                     LAAGANGDAERLYGAAIDLLADLHRHPAPGNLASYDDDAYLAEADLMLDWFMPAIPGA
                     PAAEPLRRSYHQVWQEALPLAALGPPVLVLRDYHADNLIWLPGRKGVARVGLLDFQDA
                     RAGSPAYDVVSLLEDCRRDVPPDLAEQMIGRYLDAMAEIDRAAFRAAYAVLGAQRNAK
                     IVGIFTRLWRRDGKPAYLGLIPGVWRLLEGDLAHPALEAVRAWFDRHLPPPLRRAPVP
                     "
     gene            3248..3970
                     /locus_tag="VGB88_07465"
     CDS             3248..3970
                     /locus_tag="VGB88_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9647322.1"
                     /translation="MSDAAPPRRAMVLAAGRGERLRPLTDSTPKPLIEIDGRSLLDRT
                     LDGLARAGVERAVVNVSHLADRIEAHLARRTRPAVTVSREPTLLGTGGGVANALAELG
                     DAPFYVANSDVIVLDGVRPALDRLAREWREAAMDALLLVHPTVRAFGYTGPGDYVVSP
                     DGALRRRGEREIAPCLFTGVQILHPRLFDACPEGPFSLNLMFDRAETARRLYGVVHDG
                     AFLHIGTPAALADAERYLRELD"
     gene            4135..7116
                     /gene="addB"
                     /locus_tag="VGB88_07470"
     CDS             4135..7116
                     /gene="addB"
                     /locus_tag="VGB88_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEX9647323.1"
                     /translation="MAGPAKVFTIPPGAAFVDALAEGLLAEHGGDPLALAAVTVLLPT
                     RRAARALADAFLRLAGGGVMMLPAMRAIGDVDDEEVLLADGEVLAETLGGDDLPPAIP
                     DLERQLLLAQLIRGWEGRYRGGPGPSTQAQAVRLAVELGRFLDQVQTARLTFDRLDEL
                     VTGELAAHWQETVRFLSIVTHHWPALLAARGAIDPAERRNRALAALAARWRAEPPATP
                     VIAAGSTGSIPATAELLAVVAAMASGRVVLPGLDTGLDEASWQALDESHPQFGLKLLL
                     ERLALARADVAVWPHGAARSKAAAARAALVCEAMRPEATAERWRDLGHLDAAAVRGLR
                     RIVCPEPQAEAGAIALMMREALEERGRTAALVTADRALARRVRAELLRWGVGVDDSAG
                     EPLADTVPGTFLALCLQVVAERAAPVPLLALLKHPLAAGGLAPAAFRARARRLERACL
                     RGPRPAPGFAGLKRALRARARGQRDLTQWLDALARAARPLERVLARRRIDIARLAEAH
                     LAFAEWLAGDESGGGAARLWAGEAGAAAAALFEQLIESTAGLAPIPGAEYPALVAGLV
                     ERVVVRARAGRHPRLFIWGPLEARLQHADLVVIGGLNEGTWPRAAAIDPWLSRPMRRA
                     FGLPQPERMIGLAAHDFAELASAPEVVLTRSAKVDGTPTVPSRWLARIETVLAGAGLD
                     GHLDENAFWPAWQRLLDQPQGPPRPVPPPAPRPPVAARPRRLSVTRIERWMRDPYEIF
                     ARHVLALEALEPIDAAIGVAEFGSYIHRALERFVAECAGGEPSLQRLLDIGAETLRPA
                     RAFPGLWAFWWPRFQRIAAWFVEVERARSADLVASATEVKGELDIAAPAGRFTLSAIA
                     DRIDRRKGGGLDIIDYKTGAAPSAREIAAGFAPQLPLEAAIARAGGFRGIAPAPVASL
                     AHWRLAGREPPGEVIAVAEPPARLAEQALAGLAALIATFDDPATPYVARPAPGHAPRY
                     SDYEHLARIGEWGAVERDEQW"
     gene            7110..10550
                     /gene="addA"
                     /locus_tag="VGB88_07475"
     CDS             7110..10550
                     /gene="addA"
                     /locus_tag="VGB88_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HEX9647324.1"
                     /translation="MVGALAARTRALSPEAAQRLASDPHASVWVAASAGTGKTKVLTD
                     RVLRLMLGEGDAPPAPPQRILCLTFTKAAAAEMANRLYRSLGRWAIESDRDLAEDLEK
                     LTGAGPDADTLARARRLFARVLDAPGGMRIQTIHSFCQALLARFPLEAGIPPHFEVID
                     ERTAGELMAEVQEGLAAAVEPGGGDALGRAFAAIAGHVHELTFPALLAELAGARGRIE
                     RLVRRHGGSDGLIAALARRLELAPGASGESVIAAACAEGAFDRAGLTAACRTLAAGQK
                     SDREAAALIAPWLDAADAEARVRLFAGYRRAFLTGTGKLRARLCTKAVADADAGAAAA
                     LAAEAERIDRAAERGRRAAVLGATAALITLGTALLDGYQAHKRAHGWLDYDDLILGAR
                     SLLGAGGRTAWVLYKLDGGLDHILIDEAQDTNPDQWQVVAALAEEFFAGEGARELRRT
                     VFAVGDAKQSIFSFQGADPRAFAEFRSHFARRVRDAERDWREVGLDVSFRSTAAVLDA
                     VDATFADPAARDGLDDGGDIRHRSARAGQAGLVELWPPLAPEPADAPEAWKPPVERRP
                     PDSPRARLAELVAAKIARLCDGERLESSARAIEPGDVMVLVRRRNSFMDELIRRLKER
                     AIPVAGIDRLVLTDHIAVMDLVSLGRFLLLPDDDLSLAEVLKSPLCGLDDDDLFDLAH
                     GRGEASLWQRLRARGGFATAMLEALLARVDLASPFELYARVLGADRGRERILSRLGAD
                     ASDPLDEFLAQALAYERGHVPSLQGFLHWLETGRTEIKRDLDVGRGDSVRVMTVHGAK
                     GLQAPVVFLPDTLQVPRHRAQLAWAPDGDAVLWAPASDVRDSLSAAWREQAAARGLAE
                     YRRLLYVAMTRAADRLYVCGWETKRSPPEDCWYRLVQAGLAAGATAETVADAALGFTV
                     VRLRTDQTAPPEPEPPARAAPRMAPLPDWIRAAPPAEPVPPRPLVPSAPVEAEPPARS
                     PRAAEDRAAATRGRLIHRLLQHLPELAPAARAAACRRFLARPSLALADAEQEAIARSV
                     LAVLDDPAYAAAFAPASRAEVAIAGALPGAGGRVLAGQVDRIAVSAAEVLVVDYKTGR
                     APPARPEDVPGAYLRQMAGYRAGLCAIYPDRPVRCALLWTEGPRLMALDDALLDAHAP
                     "
     gene            10670..10993
                     /gene="trxA"
                     /locus_tag="VGB88_07480"
     CDS             10670..10993
                     /gene="trxA"
                     /locus_tag="VGB88_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX9647325.1"
                     /translation="MALQHVSDQSFEDEVLKSETPVLVDFWAEWCGPCKQIAPLLEEI
                     ASEFDGRISVAKLNIDENPQTPTKYRVRGIPTLLLFKGGEVAAMKVGSMPKSALTDWV
                     ESNIA"
     gene            complement(11024..12331)
                     /locus_tag="VGB88_07485"
     CDS             complement(11024..12331)
                     /locus_tag="VGB88_07485"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX9647326.1"
                     /translation="MRHVDAVLDRLLHLHPKSIDLSLERIARLLVRLGRPERRLAPVV
                     HVAGTNGKGSVIAYLRAILEAAGQVVHVYTSPHLVRFAERIRIAGRLIEDDALAALLE
                     ECEQANAGQPITFFEITTAAAMLAFARRDADFALLETGLGGRLDATNVVTRPRLAAIT
                     PIALDHQQFLGETLAEIAAEKAAIIKPGVPCVVGRQPPEAAAVIARRAAAVGAPTLAL
                     GTDWSVRADEAGFGYRSGATSRALPTPSLPGPHQIDNAGVAVACIEQLAAGGVRVPDA
                     AVAAGLAAADWPGRLQRLATGALACLAGPGIELWLDGGHNPAAGVALAASVRALWDGD
                     PVRLVVGMMNTKDVAGFLEPLAPVAASLDAVAIPGQANALPPAAVAAAAAAHGIPARV
                     APDVPGAIAYHAARGEPAHRMLICGSLYLAGAVLALNRTPPRD"
     gene            complement(12328..13281)
                     /gene="accD"
                     /locus_tag="VGB88_07490"
     CDS             complement(12328..13281)
                     /gene="accD"
                     /locus_tag="VGB88_07490"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX9647327.1"
                     /translation="MSWLSNFVRPKIRALVARPDVPENLWDKCPSCEQMIFHRDLEAQ
                     LNVCPHCEHHMAIAPERRLAALFDDGAFQQIELPAVPADPLKFRDSRRYSERLRDAQA
                     ATGRADALVVVHGQLGGRPAVACAFDFAFMGGSMGLAVGEAFIAAARLAVLKRAPLVV
                     FPATGGARMQEGILSLMQMPRTTVAVGEVREAGLPYIVVLTNPTTGGVTASLAMLGDI
                     TLAEPGALIGFAGPRVIESTIRQRLPEGFQRAEYLLEHGMVDAVVHRRELRAVLIRVI
                     GLLTDPLPRAEVVDLVERGIDMPERAPEPAPGAADPGDAQT"
     gene            complement(13285..14133)
                     /gene="trpA"
                     /locus_tag="VGB88_07495"
     CDS             complement(13285..14133)
                     /gene="trpA"
                     /locus_tag="VGB88_07495"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9647328.1"
                     /translation="MSGPTNSRRRIERRFAEIADQGRGGLVAFVMAGDPDFEAAGAIL
                     SGLPAAGADVIELGMPFTDPMADGPAIQAAGQRALKAGQTLKRTLALVRDFRALDDAT
                     PVVLMGYYNPIYVYGVDRFLADAGSAGVDGLIVVDLPPEEDVELCLPSLKAGLCFIRL
                     ATPTTDDRRLPTVLTNTSGFLYYVSITGITGAAGAGENVIAAAVGRLKRHTSLPVAVG
                     FGIKTPDQAAAVARHADAAVVGSALVERVRAALDDGGDPVAAVLDLVAALAGGVRRAR
                     LVKTKG"
     gene            complement(14130..15344)
                     /gene="trpB"
                     /locus_tag="VGB88_07500"
     CDS             complement(14130..15344)
                     /gene="trpB"
                     /locus_tag="VGB88_07500"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9647329.1"
                     /translation="MTTDINSYREGPDEHGHFGIFGGRYVAETLMPLILDLERAYREA
                     RADPAFEAELASLLTHYVGRPSPLYLAERLGARLGGARIYFKRDELNHTGAHKINNCV
                     GQILLARRMGKTRIIAETGAGQHGVATATVCALFGLPCTVYMGAVDIERQQPNVFRMR
                     LLGAEVRTVTSGSATLKDAMNEALRDWVTNVENTYYIIGTVAGPHPYPELVRDFQSVI
                     GREARAQVLEREGRLPDTLVACIGGGSNAIGLFHPFLDDREVRMIGVEAGGEGIETGR
                     HAASLTAGRPGVLHGNRTYLLQDDDGQIKEAHSISAGLDYPGVGPEHAWLHDIGRVDY
                     VSVTDREALEAFRMCAELEGIIPALEPAHALAHVIRIAPALARDHILVMNMCGRGDKD
                     IYTVAKALGVEL"
     gene            complement(15382..16062)
                     /locus_tag="VGB88_07505"
     CDS             complement(15382..16062)
                     /locus_tag="VGB88_07505"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9647330.1"
                     /translation="MAAVSAKICGITEVGALDAAVGGGARWLGFVFFPPSPRNLGIER
                     AADLARRAPETTGRVGVFVEPSDDELAQVLARVPLTALQLHGAESPARVGALRARCCL
                     PVIKAVPVAGADDLARARAYESCADWLMFDGKAPSAARAGGLPGGNAVAFDWRLLAGT
                     WWRLPWLLSGGLDAANVAEAARVSGARHVDVSSGVESAPGVKDPARIRAFLAAIADIG
                     AAAERAAE"
     gene            complement(16067..16798)
                     /gene="pyrF"
                     /locus_tag="VGB88_07510"
     CDS             complement(16067..16798)
                     /gene="pyrF"
                     /locus_tag="VGB88_07510"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9647331.1"
                     /translation="MTSAGRADRIICAIDTEDLERASGLAAALVGRVGAVKLGLEFFA
                     AHGPAGVRAVAAHGLPVFLDLKLHDIPNTVAGAVRMALGCAPMMLTLHAAGGAAMMRA
                     AAAAALRLADAAGAPQRPKLVAVTVLTSFDAGDLAAVGMQGPVEDQVRRLAALAQDCG
                     LDGVVASAHEVAALRRQCGADFTLVVPGIRPGWAGADDQKRIVTPSEALRQGADFLVV
                     GRPITRADDPAAAAARIADELAQAA"
     gene            complement(16868..17173)
                     /locus_tag="VGB88_07515"
     CDS             complement(16868..17173)
                     /locus_tag="VGB88_07515"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX9647332.1"
                     /translation="MRLVVRLLAIACALAAIAFALANRAPVTVSLAPLPVRMDLPVYL
                     LVLASLAVGVLAGGTASWIVAGRRRRAERSARRQLAAAERALTDARTHRAGDGAGLA"
     gene            complement(17211..17492)
                     /locus_tag="VGB88_07520"
     CDS             complement(17211..17492)
                     /locus_tag="VGB88_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX9647333.1"
                     /translation="MTKSELIQVLAQDNPHLFHRDVERIVSTVFDEIAAALARGDRVE
                     LRGFGAFSVKDRASRVGRNPRSGAAVRVPEKHVPYFKSGKELRERVNRA"
     gene            complement(17580..18503)
                     /gene="sppA"
                     /locus_tag="VGB88_07525"
     CDS             complement(17580..18503)
                     /gene="sppA"
                     /locus_tag="VGB88_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX9647334.1"
                     /translation="MALDSDALIDRRRLKRRLTLWRLVALVAIVALVAVAAGRAPQLS
                     KISRSARIAVFHVDGVILSDPYRDEAVRDLVDDDRVAAMIVRIESPGGTTFGSESLYR
                     ALRAVAEVKPVVAVMDGVAASGGYMAALAADYVVARESTITGSIGVILEATNFVGLMD
                     KLGIESESIKSGPLKAEPNPLSRMSPEARAATKALVEDVHDMFVRMVAERRQMDGARA
                     RALADGRVYTGAMAVDVGLIDGLGGVAEALTWLEEQRGIDPELPQVDVEIEYPDRLID
                     RLISAAFGKSHLTERLKLDGLVSVWHPLVTE"
     gene            complement(18663..20486)
                     /gene="rpsA"
                     /locus_tag="VGB88_07530"
     CDS             complement(18663..20486)
                     /gene="rpsA"
                     /locus_tag="VGB88_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX9647335.1"
                     /translation="MSNAAASHTAMPGIDENFEALFRESVEARDKVEGTVVLGTVIAI
                     DNEMAVIDVGLKAEGRVALKEFTGPGEVAVPKVGDRVEVFLDRIENRRGEAVLSRERA
                     RREEAWVHLERAFETNDRVEGVIFGRVKGGFTVDLGGAVAFLPGSQVDVRPVRDITPL
                     LNTKQPFQILKMDRRRGNIVVSRRAVMEETRAEARAELVASLKEGQVLEGVVKNITDY
                     GAFIDLGEVDGLLHVTDIAWRRINHPSEVLNIGQTVKVQVIRFNPETQRISLGMKQLE
                     ADPWEGVDAKYPVATKFKGRVTNITDYGAFVELEPGVEGLVHISEMSWTKKNVHPGKI
                     VATSQEVEVMVLDVDPQKRRISLGLKQCVANPWQAFAAGYPAGTEIEGEIKNITEFGL
                     FVGLAGDIDGLVHLSDLSWDRPGEEAIKEFRKGATVKAKVLEVDVDKERISLGIRQLV
                     EDPFDAALRTFKKGAVVTGVVAAVQDNGIEVTVGGATGFIRRADLARDRGDQRPDRFA
                     VGDKLDAKVTQIDRAQRKLTLSVKALEIAEEKQAMAEYGSSDSGASLGDILGAAIKAK
                     AEDSATDARADGEDAQAEDDDDEASPGAEADPEAAKAKDTE"
     gene            complement(20572..21210)
                     /gene="cmk"
                     /locus_tag="VGB88_07535"
     CDS             complement(20572..21210)
                     /gene="cmk"
                     /locus_tag="VGB88_07535"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9647336.1"
                     /translation="MIIAIDGPAAAGKGTLAKRLAERFGLAHLDTGALYRAAALRLLS
                     AGGDPADAAAARRAAEAVTAADIAAPALREETVGAAASVVAAHPGVRAALVEVQRRFA
                     RCPPGGAKGAVLDGRDIGTVILPDAEVKIFVTASLEARIERRHKELLGRGAQSIRSAV
                     LRELSERDERDRARTVAPMVPAPDALLLDTTHLDADAAFAAAVDYVLARQQE"
     gene            complement(21434..21629)
                     /locus_tag="VGB88_07540"
                     /pseudo
     CDS             complement(21434..21629)
                     /locus_tag="VGB88_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(21626..21889)
                     /locus_tag="VGB88_07545"
     CDS             complement(21626..21889)
                     /locus_tag="VGB88_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HEX9647337.1"
                     /translation="MSDQDDTKAGRVYAVRGEWDAEAGVWIATSDDVPGLVAEADTLE
                     KLIEEVKLVAPDLLELNCGVRGPAEVCLVVTSRREEKVHVPAE"
     gene            complement(21963..23303)
                     /gene="aroA"
                     /locus_tag="VGB88_07550"
     CDS             complement(21963..23303)
                     /gene="aroA"
                     /locus_tag="VGB88_07550"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9647338.1"
                     /translation="MVQPLTSRPAGALVGQVRAPGDKSISHRALMLAALSAGGTVIDG
                     LLEAADTLATARALRLLGAGVERTAPGAWRIAGVGIGGLGPPADVLDLGNSGTAARLL
                     MGMLATHPLCAVVTGDESLRARPMARVTAPLRRFGARFACANGERLPITVLGTGDAVP
                     IEYRLPVPSAQVKSAVLLAGLNAPGRTTVIEPVASRDHTERMLRLFGAEVAVAADADG
                     ARVVALTGQPELRATRLEVPGDPSSAAFPLIAALLVPGSEVTLRGVGVNPLRTGLYDT
                     LREMGADLDIAPTGEQCGEPVADITARASRLSGITVPAARAARMIDEYPILAVAAACA
                     DGVTRLEGLGELRVKESDRLAAIEAGLAANGVAVEAGPDSLTITGCAGPPPGGGVVKT
                     HMDHRIAMAFLVLGLAARAPVTVDDAGMIATSFPGFAELMGGLGAAIVQEREPS"
     gene            23456..23824
                     /locus_tag="VGB88_07555"
     CDS             23456..23824
                     /locus_tag="VGB88_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX9647339.1"
                     /translation="MAKAVLGQKHSCRECGVKFYDLARQPPTCPACGAPIQSTDKPRP
                     RRAPAPEPRKAVTAVAAPEVEEVALDEDLEIETEDLEKEIGDDIEAEDEEEGGDLIED
                     ASELGEDDTTVAGVVGEDER"
     gene            23928..24003
                     /locus_tag="VGB88_07560"
     tRNA            23928..24003
                     /locus_tag="VGB88_07560"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23961..23963,aa:Ala,seq:ggc)
     gene            24239..24514
                     /locus_tag="VGB88_07565"
     CDS             24239..24514
                     /locus_tag="VGB88_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647340.1"
                     /translation="MHRPAIVLACVLCAAQAQAQEALTPEQAKCILDYIDAAKTAQGA
                     RDIRTACVDYRGKDPELFSCVIKQQVKAHSDAGADAIREACQAMKTQ"
     gene            complement(24591..25271)
                     /locus_tag="VGB88_07570"
     CDS             complement(24591..25271)
                     /locus_tag="VGB88_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9647341.1"
                     /translation="MAQQQVQSAVGVGDPAPEFTLPGLDPAREVTLSAYRGRSNVLLG
                     LFRGLHCPFCRRQVALLNGIAPRLNDEGIKVVAVINTEPARMRGYYRSQTVRIDIGAD
                     PDWRTHARYGLGRLQATEGPTDWPRGLVNMDEFMALKVDPTGELDAPMSPVEANWALN
                     KKDGFELDAVDQRIFDQHGMTGAGHFLIDRDGVVRWAWREGEAGMASMATFPTRQQIL
                     DAVLGASR"
     gene            complement(25362..27848)
                     /locus_tag="VGB88_07575"
     CDS             complement(25362..27848)
                     /locus_tag="VGB88_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9647342.1"
                     /translation="MSEQASQPHAATRADLAAALWHADVAVAVWNDEDRLVAFNRRFV
                     ELIPEVADQVRPGVPFDALLYRAIQAGAVPAARHDSKDWMRRRIAAHRAPGAAFEVET
                     GLGRWLRVSETRLADGSTVSTWTDITELKRDALQHRLNEERYRSLVDNAPVTLLVHAD
                     GRVVFVNQEGARRFAAAAPEALIGRPVASLLDRGAGPASAAEAVLAGEAAPVEAAAGV
                     LRFRRLDGAPLWLAVQARRLDGADHDLVLVSGSDVTGLQAASAEAATAVERLATAIDG
                     VPELIALFDADDRLVLANRASRAIEAALGELWRPGVDYETYLRAAVARGLYPQAEGCA
                     DDWIARRLDQHRSPSGPVELARQDGRWLSVNERRLADGSVISIASDVTEHKSNAQQIR
                     FLAHHDALTGLPNRSLFQDRLEQALALARRDGRKVAVLLLDLDHFKHINDNLGHAAGD
                     RVLVEVAARLRGCARGGDTVARLGGDEFAVIQTQVADAEQAAALAQRALAALSGPVVI
                     DGQPVHTGASVGITLFPDDAGELGELMKNADLALHRAKALAPGRLEFYASEMTRQAQQ
                     RMAVTEGLRRALAEDQFVLHYQPTIRLADGVVIGGEALLRWHRPEQGLVAPAEFIAHA
                     EATGLILALGEWALDRCCTQLAEWARKGHLMVPVWVNVSAAQFRDQALLDKVRASLLG
                     AGVEARMLGLEITESALMPDVAQATATLNRLVELGVELSIDDFGTGYSSLNYLKKFPV
                     GKLKIDQSFVRDITSNPLDSAIARAIIHLGHSLGMHVLAEGVETTRQANLLSDLGCDE
                     IQGRLVSAPLPADEFVRFVARAAGLREPRL"
     gene            28046..>28642
                     /locus_tag="VGB88_07580"
     CDS             28046..>28642
                     /locus_tag="VGB88_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9647343.1"
                     /translation="MCGRYSLTSPAEAMVALFDAGPLAGFQPRYNVAPSQAMPVVRHA
                     EAGGREWAWLRWGLIPSWAKDPAIGNRMINARSESVADKPAFRAAFRRRRCLVPADGF
                     YEWQQQGGVKQPWRITLAEGGPFAFAGLWERWSPGAGEAVETYTILTTAANRSLAPIH
                     PRMPVILAPEHYAVWLGDGQAADPRLLEPLLAPYPDARL"
BASE COUNT         3668 a        10603 c        10436 g         3935 t
ORIGIN      
        1 cgaggcgcgc caccggcgcc tatgtcgcct tcaccgtggc gatggtgctg gtgatgatct
       61 tcccggtcta cggcctcggc aaccgggtcg agccgttcgt gctcggcctg ccgtggtcgc
      121 tcgcctgggt ggtgttctgg atcggggtcg agttcgtcgg cctgatcgcg ttctacctct
      181 acgagcacgg cggcgagggg ggctgaggcg atggcgcagt gggtcgtcgc gctcggcgcg
      241 atgtcggcct atctcgcctt tgccttcgcg ctcggcatcc tcgccgggcg caaccgctcg
      301 ttcttctcgg tctcggaata cgcggtcgcc gaccggggcc tcgggctggt ggtgatgtgg
      361 ttcctgatgg gcggcaccat cttctcggcc ttcgccttcc tcggcgggcc gggatgggcg
      421 tacgagaagg gcgcggcgtc gttctacatc ctcgcctatg cctcgctcgg gctgctgccg
      481 tggtacctga tcgggcccaa ggtcgcgcgc ctcggccggg cgggcaacta cttcaccatg
      541 ggcgacctgc tcgccgaccg ctaccgctcg cgggcgctgg tggtcctggt cggcgtcgtc
      601 tcggtgctcg ccttcatcca gtacctgacc ctgcagatca agggcatggc ctacgtgttc
      661 agcgtgctca ccgagggcgc ggtgccgttc tggctcggcg cgctgctggc ctacggcatc
      721 gtcgtggtct acgtcgccac cagcggcgtg cgcggcgcgg cgtggagcga cgtcctccag
      781 ggcatcctga tgctggcggt cgcctgggtg ctcggcctgt ggctggtcac cacgctccac
      841 ggcgggctcg ggccgatgtt cgccgcgatc gccaccgagc ggccgggcct gctggtgatc
      901 ggcgccgagg gctcggcgat gtcggcgacg gcgtactcga gcgcgatcgt cgtctcgacc
      961 ctcggcttca tgatgtggcc gcatctgttc atgaagtcct acaccaccag cgagcggcgc
     1021 ataaagctca ccgtgctcgc ctatccggtg ttcgccatct tcctcgtgcc ggtgctcctg
     1081 atcggcttcg cggcgatcgg cgtggtcgcg cccgagtcgc tcgccagcac ggaccaggtg
     1141 ctgccgcatc tgatcaccta cgagatcggc gcctcgggcc tggtctacgg gctgatcggg
     1201 gcgggcgcct tggcggcggc gatgtcgtcg tcggacgcca tcacccacgg cgcctcggtg
     1261 tcggtcgggc gcgacatcct ggtgccgctc aatcccgccg ttgccgagcg cacccagctc
     1321 tgggtgatgc gcgcggccgt ggtcggcgtc ggcgccatcg cctattacct ggcgatcttc
     1381 ggggcagagg ggctgatcca gctgctgctc ggcgcctacg gctcgatcgt ccagttcgcg
     1441 cccgcggtct atggcgcgct ctactggcgc cgcggcacag gcgccggcgc gctcgccggg
     1501 ctcgcggccg gggtcgcggt caacttcacc ttccagctcg gcgcgccggc gccgccgctc
     1561 gacatccatc cgggcctgct cgggctggcg gtgaacctgg cggtgttcgc ggccgtgagc
     1621 ctgctgacca ggccggccga gaggtcgctg gtcgtgcgct tcgtcgacgc ctgaggccgc
     1681 gtgccgcgcc ggcggttgcc ggggcgatgc cgcgggggct agaaagcgcg ccatgcagac
     1741 caaggccagg accgaccccg gcaccaacga ggcgatcgag atcacgctcg ccgacgaggc
     1801 ggccacccgg cgcctcgccg ctgcggtcgc ggcggtcgcg cgcgcgagcg acgtgatcgc
     1861 tctctcgggc ccgctcggcg ccggcaagac ctcgttcgcg cgcggcttcg tggacgcgct
     1921 cggcgccacc gaggaggtgc cgagcccgac cttcaccctg gtccagacct atgccctggc
     1981 gccggcggcg gtgtggcact tcgacctcta ccgattggag cggccgaccg atgcctacga
     2041 gctcggcatc gaggacgcgt tcgacgccgg catcagcctg atcgaatggc cggcgcggct
     2101 gggcgcgctg ctgcccgccg accggctcga tgtggcgctc gacttcgctg ccggcgaggg
     2161 cacgggccgc atcgggcgcc tgatcggcca tggcgcctgg cacgcgcgcc ttgcggcagt
     2221 catggccagc tgggccgcgg ccggcgatgg ctgaggcggg cgcgcgcgcg gcgctgatcg
     2281 agcggttcct cgccgcccac ggctgggccg gcgggcggcg cacgccgctt gccggcgacg
     2341 cctcgttccg ccgctaccag cgcctgcgcc ggggcctgcg ccgggcggtg ctgatggacg
     2401 cgccgcccgg gcacgaggac gtgcgcccgt attgccgcat cgcccgccac ctgaccggcc
     2461 tcggctacag cgcgccggcg atctatgccg aggacgcgga cgccgggctg ctgctgatcg
     2521 aggacctcgg cgacgacacc tattcgcggc tgctggcggc gggcgcgaac ggcgatgccg
     2581 agcggctgta cggcgcggcg atcgacctgc tcgccgacct ccaccgccac cccgcgcccg
     2641 gcaatctcgc gtcttacgac gacgacgcct atctcgccga ggccgacctg atgctcgact
     2701 ggttcatgcc cgccatcccg ggcgcgcccg cggccgagcc gcttcgccgg tcctaccatc
     2761 aggtgtggca ggaggcgctg ccactggccg cgctcggccc gccggtgctg gtgctgcgcg
     2821 actatcacgc cgacaacctg atctggctgc cggggcgcaa gggcgtggcc cgcgtcggcc
     2881 tcttggactt ccaggacgcg cgcgcgggct cgcccgccta cgacgtcgtc tcgctgctcg
     2941 aggactgccg gcgcgacgtc ccccccgacc ttgccgagca aatgattggt cggtaccttg
     3001 acgcgatggc agagatcgac cgggcggcgt tccgcgccgc ctatgccgtg ctcggcgccc
     3061 agcgcaacgc caagatcgtc ggcatcttca cccgcctgtg gcggcgcgac ggcaagccgg
     3121 cctatctcgg cctgatcccc ggggtctggc gcctgctcga aggcgacctg gcccatccgg
     3181 cgctggaagc ggtgcgcgcg tggttcgacc gccatctgcc gccgccgctc aggcgcgcgc
     3241 cggtaccgtg agcgacgccg cgccgccgag acgcgccatg gtgctcgccg ccggacgcgg
     3301 cgagcgcctg cgcccgctca ccgactcgac gccgaagccg ctgatcgaga tcgacggccg
     3361 cagcttgctc gaccgcacgc tcgacgggct cgcgcgcgcc ggtgtcgagc gcgcggtggt
     3421 caacgtcagc cacctcgccg acaggatcga ggcgcatctc gcccggcgca cgcggcccgc
     3481 ggtcacggtc agccgcgagc cgaccctgct cggcaccggc ggcggcgtcg ccaacgcgct
     3541 cgccgagctc ggcgatgcgc cgttctacgt cgccaacagc gacgtgatcg tgctcgacgg
     3601 cgtgcgcccg gcgctcgaca ggctggcccg ggagtggcgc gaggcggcca tggacgcgct
     3661 gctgctggtc caccccacgg tgcgcgcgtt cggctatacc ggccccggcg actacgtggt
     3721 cagccccgac ggcgcgctca ggcgccgggg cgagcgcgag atcgcgccgt gcctgttcac
     3781 cggcgtccag atcctgcatc cgcggctgtt cgacgcctgc cccgaggggc cgttctcgct
     3841 caacctgatg ttcgaccgcg ccgagacggc gcggcgcctg tacggcgtcg tccacgacgg
     3901 cgccttcctg cacatcggca cgcccgcggc gctggccgac gccgagcgat atctgcgcga
     3961 gctcgactga gagtgtttgg atgactcgag tgccgtccta cgagacgctc gcttcgctcg
     4021 ctcctcagga cgaggggggg ttgagaatca atcatttgcc tcatcctgag gagcgcccca
     4081 agggcgcgtc tcgaagggtg aggcaaatga ttcgcaagct gtgagggcgg gccgatggcg
     4141 ggccccgcca aggtgttcac catcccgccc ggcgccgcgt tcgtcgacgc gctcgccgag
     4201 ggcctgctcg ccgagcacgg cggcgatccc ttggcgcttg ccgcggtgac cgtactgctg
     4261 ccgacccggc gcgcggcgcg cgcgctcgcc gatgcgttcc tgcgccttgc cggcggcggg
     4321 gtgatgatgc tgccggcgat gcgcgcgatc ggcgacgtcg acgacgagga ggtgctgctc
     4381 gccgacggcg aggtactcgc cgagacgctc ggcggcgacg atctgccgcc ggcgattccc
     4441 gacctcgaac ggcaattgtt gctcgcgcaa ctgattcgcg gctgggaagg caggtatcgc
     4501 ggcggcccgg gcccctcgac ccaggcgcag gccgtgcggc tcgcggtcga gctcggccgg
     4561 ttcctcgacc aggtgcagac cgcgcggctc accttcgacc gcctcgatga gctggtcacg
     4621 ggcgagcttg ccgcccactg gcaggagacg gtgcggttcc tgtccatcgt cacccaccac
     4681 tggcccgcgc tgctggccgc gcgcggtgcg atcgacccgg cggagcgacg caaccgcgcg
     4741 ctcgcggcgc tcgcggcgcg ctggcgcgcc gagccgccgg cgacgccggt catcgccgcg
     4801 ggctcgaccg gcagcatccc ggcaaccgcc gagctgctgg cggtggtcgc cgccatggcg
     4861 agcggccggg tggtgctgcc ggggctcgat accggcctcg acgaggcgag ctggcaggcg
     4921 ctcgacgaga gccatccgca gttcgggctc aagctgctgc tcgagcgcct cgccctcgcg
     4981 cgcgcggacg tagccgtgtg gccgcacggc gcggcgcgca gcaaggcggc ggcagcgcgc
     5041 gcggcgctgg tctgcgaggc gatgcgcccc gaggccaccg ccgagcgctg gcgcgacctc
     5101 gggcacctcg acgccgccgc ggtccggggt ctcaggcgca tcgtctgtcc cgagccccag
     5161 gccgaggcgg gggcgatcgc gctgatgatg cgcgaggcgc tggaggagcg cgggcgcacc
     5221 gcggcgctgg tcaccgccga ccgcgccctt gcccgccggg tgcgcgccga gctgctgcgc
     5281 tggggggtgg gggtcgacga ctcggccggc gagccgctcg ccgacactgt ccccggcacg
     5341 ttcctcgcgc tgtgccttca ggtagtcgcc gagcgggcgg caccggtgcc gctgctcgcg
     5401 ctgctcaagc atccgctcgc cgccggcggc cttgcgcccg ccgcgttccg ggcgcgggcg
     5461 cggcggctcg agcgcgcctg cctgcgcggc ccgcgtccgg cgccgggctt cgccgggctc
     5521 aagcgcgcgc tgcgcgcgcg cgcgcgcggc cagcgcgacc tcacccaatg gcttgatgcg
     5581 ctggcgcgcg ccgcccggcc gctcgagcgc gtgctcgccc gccggcgtat cgacatcgcc
     5641 aggctcgccg aggcgcacct cgccttcgcc gaatggctgg ccggcgacga gtcgggcggc
     5701 ggcgcggcgc ggctgtgggc gggcgaggcc ggggcggcgg cggcggcgct gttcgagcag
     5761 ctgatcgagt ccacggcggg cctcgcgccg atccccggcg ccgagtaccc ggcgctggtc
     5821 gcggggctgg tcgagcgcgt ggtggtgcgc gcgcgcgccg gccgccatcc gcggctgttc
     5881 atctgggggc cgctcgaggc gcgcctgcag cacgccgacc tggtggtcat cggcgggctg
     5941 aacgagggca cctggccgcg cgcggcggcg atcgacccgt ggctcagccg gccgatgcgc
     6001 cgggccttcg gcctgcccca gcccgagcgc atgatcggcc tcgccgccca cgacttcgcc
     6061 gagctcgcct ccgcgcccga ggtggtgctg acccgctcgg ccaaggtcga cggcacgccg
     6121 accgtgccgt cgcgctggct ggcgcggatc gagaccgtgc tcgccggcgc cggcctcgac
     6181 ggacacctgg acgagaacgc gttctggccg gcctggcagc gcctgctcga ccagccgcag
     6241 gggccgccgc gcccggtccc gccgccggcg ccgcgcccgc ccgtggccgc gcgccccagg
     6301 cgcctgtcgg tgacccggat cgagcgctgg atgcgcgatc cctacgagat cttcgcccgc
     6361 cacgtgctcg ccctggaggc gctggagccg atcgacgccg ccatcggcgt tgccgagttc
     6421 ggcagctaca tccatcgcgc gctcgagcgc ttcgtggccg agtgcgcggg cggcgagccg
     6481 tcgcttcagc gcctgctcga catcggcgcc gagaccctgc gcccggcgcg cgccttcccc
     6541 ggcctgtggg cgttctggtg gccgcgcttc cagcgcatcg ccgcctggtt cgtcgaggtc
     6601 gagcgtgccc gcagcgccga ccttgtcgcc agcgccaccg aggtcaaggg cgagctcgac
     6661 atcgccgcgc cggccggccg cttcaccttg agcgcgatcg ccgaccgcat cgaccggcgc
     6721 aagggcggcg gcctcgacat catcgactac aagaccggcg ccgcgcccag cgccagggag
     6781 atcgccgccg gctttgcgcc gcagctgccg ctcgaggccg cgatcgcccg cgccggcggc
     6841 tttcgcggca tcgcgccggc gccggtcgcc tcgctcgccc actggcggct cgccggccgc
     6901 gagccgcccg gcgaggtcat cgccgtcgcc gagccgccgg cgcgcctggc cgagcaggcg
     6961 ctggccggtc tcgccgccct catcgccacc ttcgacgacc ccgccacgcc ctacgtcgcg
     7021 cgcccggcgc cggggcacgc gccgcgctac agcgactacg agcatctcgc ccgcatcggc
     7081 gagtggggcg cggtggagcg cgacgagcaa tggtaggcgc gctggcggcg cgcacgcggg
     7141 cgctgtcgcc cgaggcggcg cagcggctgg cctcggaccc gcacgcctcg gtgtgggtgg
     7201 cggcgagcgc gggcaccggc aagaccaagg tgctgaccga ccgcgtgttg cgcctgatgc
     7261 tcggcgaggg cgacgcgccg ccggcgccgc cgcagcgcat cctgtgcctg accttcacca
     7321 aggcggcggc ggcggagatg gccaaccgcc tgtaccggag cctcggccgg tgggcgatcg
     7381 agtccgaccg cgacctcgcc gaggacctcg agaagctgac cggcgccggc cccgacgcgg
     7441 acacgctggc gcgcgcgcgc cggctgttcg cgcgcgtgct cgacgcgccc ggcggcatgc
     7501 gcatccagac catccattcg ttctgccagg cgctgctcgc gcgcttcccg ctcgaggccg
     7561 gcatcccacc ccacttcgag gtcatcgacg agcgcaccgc gggcgagctg atggccgagg
     7621 tccaggaggg cctggccgcg gccgtcgagc cgggcggcgg cgatgccttg gggcgcgcct
     7681 tcgccgcgat cgccggccac gtccacgagc tgaccttccc ggcgctgctc gccgagcttg
     7741 ccggcgcgcg cgggcgcatc gagcgcttgg tcaggcgcca cggcggaagc gacggcctga
     7801 tcgccgcgct cgcccggcgt cttgagctcg cgcccggggc gagcggggag tcggtgatcg
     7861 cggccgcctg cgccgagggc gcgttcgacc gcgccgggct caccgctgcc tgccggacgc
     7921 tggcggcggg ccagaagagc gaccgcgagg ccgccgcgct gatcgccccc tggctcgacg
     7981 ccgccgacgc ggaggcgcgc gtgcgcctgt tcgccggcta ccggcgcgcc ttcctgaccg
     8041 gcaccggcaa gctcagggcc aggctgtgca ccaaggcggt ggcggacgcc gacgccggcg
     8101 cggctgcggc gctcgccgcc gaggccgagc gcatcgaccg ggcggcggag cgcgggcgcc
     8161 gcgccgccgt gctcggggcc acggctgcgc tcatcaccct cggcaccgcg ctgctcgacg
     8221 gctatcaggc gcacaagcgc gcccatggct ggctcgacta cgacgacctg atcctcggcg
     8281 cgcgcagcct gctcggcgcc ggcgggcgca ccgcctgggt gctgtacaag ctcgacggcg
     8341 gcctcgacca catcctgatc gacgaggccc aggacaccaa cccggaccag tggcaggtgg
     8401 tggcggcgct ggccgaggag ttcttcgccg gcgaaggcgc gcgcgagctg cgccggaccg
     8461 tgttcgcggt cggcgacgcc aaacagtcga tcttcagctt ccagggcgcc gacccgcgcg
     8521 ctttcgccga gttccgcagc cacttcgccc ggcgcgtgag ggacgccgag cgcgactggc
     8581 gcgaggtcgg cctcgacgtc tcgttccgct cgaccgcggc ggtgctcgat gcggtcgacg
     8641 cgaccttcgc cgacccggcg gcgcgcgacg gcctcgacga cggcggcgac atccgccacc
     8701 gctcggcgcg cgccggccag gccgggctgg tcgagctgtg gccgccgctc gcgcccgagc
     8761 ccgccgatgc gcccgaggca tggaagccgc cggtcgagcg gcgcccgccc gattcgccgc
     8821 gcgcgcggct ggccgagctg gtggcggcga agatcgcccg gttgtgcgac ggcgagcggc
     8881 tcgagtcctc ggcgcgcgcc atcgagcccg gcgacgtgat ggtgctggtg cgccgccgca
     8941 actcgttcat ggacgagctg atccgccgcc tcaaggagcg cgcgatcccg gtcgccggca
     9001 tcgaccgcct ggtcttgacc gaccacatcg cggtcatgga cctggtctcg ctcgggcgct
     9061 ttctgctgct ccccgacgac gacctcagcc tcgccgaggt gctcaagagc ccgctgtgcg
     9121 gcctcgacga cgacgacctg ttcgacctcg cccacggccg cggcgaggcc tcgctgtggc
     9181 agcggctgcg ggcgcgcggc gggttcgcga cggcgatgct ggaggcgctg ctcgcccgcg
     9241 tcgacctcgc ctcgccgttc gagctctacg cccgcgtgct cggcgccgac cgcgggcggg
     9301 agcgcatcct gtcccgcctc ggcgccgacg cgagcgaccc gctggacgag ttcctggccc
     9361 aggcgctggc ctacgagcgt ggccacgtgc cgagcctcca gggcttcctg cactggctcg
     9421 agaccggccg caccgagatc aagcgcgacc tcgacgtcgg ccgcggcgac tcggtgcggg
     9481 tgatgaccgt gcacggcgcc aaggggctgc aggcgccggt ggtgttcctg cccgacaccc
     9541 tgcaggtgcc gcgccatcgc gcccagctcg cctgggcgcc ggacggcgac gccgtgctgt
     9601 gggcgccggc gagcgatgtg cgcgattcgc tgtcggcggc gtggcgcgag caggcggcgg
     9661 cgcgcggcct ggcagagtac cggcgcctgc tctacgtcgc catgacccgc gcggccgatc
     9721 gcctgtatgt gtgcggctgg gagaccaagc ggagcccgcc cgaggactgc tggtatcgcc
     9781 tggtccaggc cgggctcgcc gccggcgcca cggctgagac ggttgcggac gccgcgctcg
     9841 gcttcaccgt ggtgcggctg cgcacggacc agaccgcccc gcccgagccc gagccgccgg
     9901 cgcgggccgc gccgcgcatg gcgccgttgc ccgactggat ccgcgccgcg ccgcccgcgg
     9961 agcctgtgcc gccgcggccg ctggtgccct cggcgccggt cgaggccgag ccgccggcgc
    10021 gctcgccgcg cgccgccgag gaccgcgccg ccgccacccg cgggcgcctc atccaccgcc
    10081 tgctccagca cctgcccgag ctcgccccgg cggcgcgcgc ggcggcctgc cggcggttcc
    10141 tcgcccgccc ctcgctcgcg cttgcggacg ccgagcagga ggcgatcgcg cgctcggtgc
    10201 tcgcggtcct cgacgatccg gcctacgccg ccgcgttcgc gccggcgagc cgcgccgagg
    10261 tggcgatcgc cggcgcgctg ccgggcgccg gggggcgggt gctcgccggc caggtcgacc
    10321 gcatcgcggt cagcgcggcc gaggtgctgg tggtcgacta caagaccggc cgcgcgccgc
    10381 ctgcgcggcc cgaggacgtg cccggcgcgt atctgcgcca gatggcgggc taccgcgcgg
    10441 gcctctgcgc gatctacccc gaccggccgg tgcgctgcgc cctgctgtgg accgagggcc
    10501 cgcggctgat ggcgctcgac gacgccctgc tcgacgccca cgcgccgtga cttaagggct
    10561 ccgtggctgc cccggcccgc gccttgacgc tgtcatgggg ggttcctaca gtgcggtttc
    10621 ggacggcccc tcgggggccg ttgccgccag ccacggacag gagcggtgca tggccctgca
    10681 gcacgtttcc gaccagtcgt tcgaggacga ggtgctcaag tccgagaccc cggtgctggt
    10741 cgacttctgg gccgaatggt gcgggccctg caagcagatc gcgcccctct tggaggagat
    10801 cgcgtccgag ttcgacggcc gcatctcggt ggccaagctc aacatcgacg agaacccgca
    10861 gaccccgacc aagtaccggg tgcgcggcat ccccaccctg ctgctgttca agggcggcga
    10921 ggtggcggcg atgaaggtcg gctcgatgcc gaaaagcgcg ctcaccgact gggtcgaatc
    10981 gaacatcgcc tgaccgccgc gtcgtcgccg gcgacgatcc gcctcagtcg cggggcgggg
    11041 tgcggttgag cgcgagcacc gcgccggcca gatagagcga gccgcagatc agcatccggt
    11101 gcgccggctc gccgcgcgcg gcatggtaag cgatcgcgcc gggcacatcc ggcgcgacgc
    11161 gcgccgggat gccgtgggcc gccgccgcag cggcgaccgc cgccggcggc agcgcgttcg
    11221 cctggcccgg gatcgcgacc gcatcgaggc tcgccgcgac cggcgcaagc ggctctagga
    11281 acccggccac gtccttggtg ttcatcatgc cgaccaccag ccgcaccggg tcgccgtccc
    11341 acaaggcgcg cacgcttgct gccagcgcga cgccggcggc ggggttgtgg ccgccgtcga
    11401 gccacagctc gatcccgggc ccggccaggc aggcgagcgc gccggtggcg agccgctgca
    11461 gccgccccgg ccagtcggcg gcggcgagcc cggcggcgac cgccgcgtcc ggcacgcgca
    11521 ccccgccggc ggcgagctgc tcgatgcacg ccaccgccac accggcgttg tcgatctggt
    11581 gcgggccggg cagcgacggc gtcggcaggg cccggctggt cgcgccggat cgatagccga
    11641 accccgcctc gtccgcgcgc accgaccagt cggtgcccag cgcaagggtc ggcgcgccca
    11701 ccgcggccgc ccggcgcgcg atcacggccg ccgcctcggg cggctggcgg ccgaccacgc
    11761 acggcacccc gggcttgatg atcgccgcct tctcggccgc gatctcggcc agcgtctcac
    11821 ccaggaactg ctggtggtcg agcgcgatcg gggtgatcgc ggcgagccgc gggcgggtga
    11881 cgacgttggt cgcatcgagc cggccgccga gcccggtctc gagcaaggcg aaatcggcgt
    11941 cgcggcgagc gaaggcgagc atcgccgcgg cggtggtgat ctcgaagaag gtgatcggct
    12001 ggccggcgtt cgcctgctcg cactcctcga gcagcgcggc gagcgcgtca tcctcgatca
    12061 gccggccggc gatgcggatg cgctcggcga agcgcaccag gtgcggcgag gtgtagacgt
    12121 ggacgacctg gccggcagcc tcgaggatcg cgcgcaggta agcgatcacc gagcccttgc
    12181 cgttggtgcc ggcgacgtgg accaccggcg cgagccgccg ctccggccgg ccgaggcgca
    12241 cgagcaggcg cgcgatgcgc tcgagcgaaa ggtcgatcga cttggggtgg agatggagca
    12301 ggcggtcgag gaccgcgtcc acgtgcctca cgtctgggcg tcacccgggt cggcggcgcc
    12361 cggcgcgggc tcgggcgccc gctccggcat gtcgatgccg cgctcgacca ggtcgaccac
    12421 ctctgcgcgc ggcagcgggt cggtgagcag cccgatgacg cggatcagca ccgcgcgcag
    12481 ctcgcgccgg tggaccaccg catcgaccat gccgtgctcg agcagatact cggcgcgctg
    12541 gaagccctcc ggaaggcgtt ggcggatcgt gctctcgatc acccgcggcc cggcaaagcc
    12601 gatcagcgcg ccgggctcgg cgagcgtgat gtcgccgagc atcgccagcg acgcggtcac
    12661 cccgccggtg gtcgggttgg tcagcaccac gatgtagggc aggcccgcct cgcgcacctc
    12721 gcccaccgct accgtggtgc gcggcatctg catcagcgac aggatgcctt cctgcatgcg
    12781 cgccccgccc gtggccggga acaccaccag cggcgcccgc ttgagcaccg ccagccgcgc
    12841 cgcggcgatg aacgcctcgc cgaccgcgag ccccatcgag ccgcccatga acgcgaagtc
    12901 gaacgcgcac gccaccgccg gccggccgcc gagctggcca tgcaccacca ccagcgcgtc
    12961 cgctcgcccg gttgcggcct gggcgtcgcg caggcgctcc gaatagcgcc ggctgtcgcg
    13021 gaacttgagc gggtcggcgg gcaccgccgg cagctcgatc tgctgaaagg cgccgtcgtc
    13081 gaacagcgcc gcgagccgcc gctcgggcgc gatcgccatg tggtgctcgc aatgcgggca
    13141 gacgttgagc tgcgcctcga ggtcgcgatg gaagatcatc tgctcgcagc tcgggcactt
    13201 gtcccacagg ttctcgggca cgtccggccg ggcgaccagg gcgcgaatct tggggcggac
    13261 gaaattgctg agccagctca ccgttcatcc tttcgtcttg accaggcggg cgcggcgaac
    13321 gccgccggcc agcgccgcga ccaggtccag caccgcagcg accgggtcgc cgccgtcgtc
    13381 gagcgcggcg cgcacccgct cgaccagcgc cgagccgacc accgccgcgt cggcgtgccg
    13441 cgccaccgcc gccgcctggt cgggggtctt gatgccgaag ccgaccgcga ccggcaacga
    13501 ggtatggcgc ttcagccgcc cgaccgcggc cgcgatcacg ttctcgccgg cgccggcggc
    13561 gccggtgatg cccgtgatcg acacataata caggaaaccg ctggtgttgg tcagcacggt
    13621 cggcaggcgg cggtcgtcgg tggtgggcgt cgccaggcgg atgaagcaga gcccggcctt
    13681 gagcgacggc aggcacagct ccacgtcctc ctcgggcggc aggtcgacga cgatcaggcc
    13741 gtcgaccccg gcgctgccgg catcggcgag gaaccggtcg acgccgtaga cgtagatcgg
    13801 gttgtagtag cccatcagca ccaccggcgt cgcatcgtcc agcgcgcgga agtcgcgcac
    13861 cagggcgagc gtgcgcttga gcgtctggcc ggccttgagc gcgcgctggc cggcggcctg
    13921 gatcgccggg ccgtcggcca tcgggtcggt gaacggcatg ccgagctcga tcacgtcggc
    13981 gccggccgcg ggcaggccgc tgaggatcgc gcccgcggcc tcgaagtcgg ggtcgccggc
    14041 catgacgaac gcgaccagcc cgccgcggcc ctggtcggcg atctcggcga accggcgctc
    14101 gatgcgccgg cggctattgg ttggcccgct cacagttcga cgcccagcgc cttggccacc
    14161 gtgtagatgt ccttgtcgcc gcgcccgcac atgttcatga ccaggatgtg gtcgcgcgca
    14221 agcgcgggcg cgatccggat cacatgggcg agcgcatgcg ccggctcgag cgccgggatg
    14281 atgccctcga gctccgcgca catccggaac gcctccagcg cctcgcggtc ggtgaccgag
    14341 acgtagtcga cccggccgat gtcgtgcagc caggcgtgct cggggccgac gccggggtag
    14401 tcgaggccgg cggagatcga gtgcgcctcc ttgatctggc cgtcgtcgtc ctgcagcagg
    14461 taggtgcggt tgccgtgcag cacgccgggg cggccggcgg tcagcgacgc cgcgtggcgg
    14521 ccggtctcga tgccctcgcc gccggcctcg accccgatca tgcgcacctc gcggtcgtcg
    14581 aggaacgggt ggaacaggcc gatcgcgttc gagccgccgc cgatgcacgc gaccagggtg
    14641 tcgggcaggc gcccttcgcg ctcgagcacc tgcgcgcgcg cctcgcgccc gatcaccgac
    14701 tggaagtcgc gcaccagctc cgggtaggga tggggcccgg cgaccgtgcc gatgatgtag
    14761 taggtgttct cgacattggt cacccagtcg cgcagcgcct cgttcatcgc gtccttgagc
    14821 gtcgccgagc ccgaggtcac ggtcctgacc tcggcgccga gcaagcgcat gcggaacacg
    14881 ttcggctgct ggcgctcgat gtcgacggcg cccatgtaga ccgtgcacgg caggccgaac
    14941 agcgcgcaca cggtggcggt cgccacgccg tgctggccgg cgccggtctc ggcgatgatc
    15001 cgggtcttgc ccatgcgccg ggcgagcagg atctggccga cgcagttgtt gatcttgtgg
    15061 gcgccggtgt ggttgagctc gtcgcgcttg aagtagatgc gcgcgccgcc gagccgcgcg
    15121 cccagccgct cggcgagata gagcgggctc ggccggccga cgtaatgggt cagcagcgag
    15181 gcgagctcgg cctcgaaggc ggggtccgcg cgcgcctcgc gatacgcgcg ctcgaggtcg
    15241 aggatcagcg gcatcagcgt ctcggcgacg taacggccgc cgaagatgcc gaaatggccg
    15301 tgctcgtcgg ggccctcgcg atagctgttg atgtcggtgg tcatggtcct cgctcgcccg
    15361 gcatcgattg cctggacaac cttactccgc cgcgcgctcg gccgcggcgc cgatgtcggc
    15421 gatcgcggcc aggaacgcgc ggatgcgcgc cgggtccttg accccgggcg ccgactcgac
    15481 cccggaggag acgtcgacgt ggcgggcgcc cgagacgcgc gccgcctcgg cgacgttggc
    15541 cgcgtcaagc ccgcccgaca gcagccacgg aagccgccac caagtgccgg cgagcagccg
    15601 ccagtcgaac gccaccgcgt tgccgccggg aagcccgccc gcacgcgccg cactcggcgc
    15661 cttgccgtcg aacatcagcc agtcggcaca gctttcgtag gcgcgcgcgc gggcgaggtc
    15721 gtccgcgcct gcgaccggca ccgccttgat caccggcagg cagcagcgcg cgcgcagcgc
    15781 cccgacgcgc gccggcgact cggcgccgtg gagctggagc gcggtcagcg gcacgcgcgc
    15841 gagcacctgc gcgagctcat cgtcggacgg ctcgacgaac acgccgaccc ggcccgtggt
    15901 ctcgggcgcg cgccgggcga ggtccgcggc gcgctcgatg ccgaggttgc gcggcgacgg
    15961 cggaaagaac acgaagccga gccagcgcgc gccgccgccg accgcggcat cgagcgcgcc
    16021 cacctcggtg atgccgcaga tcttggccga gaccgccgcc atcagctcag gccgcctggg
    16081 ccagctcgtc ggcgatccgc gccgccgccg ccgccgggtc gtcggcgcgg gtgatcggcc
    16141 ggccgacgac caggaagtcg gcgccctggc gcagcgcctc ggacggcgtg acgatccgct
    16201 tctggtcgtc ggcgcccgcc cagcccggtc ggatgccggg caccaccagg gtgaagtcgg
    16261 caccgcactg gcggcgcagc gccgccacct cgtgcgccga ggcgaccacg ccgtcgaggc
    16321 cgcagtcctg ggcgagcgcg gcgagccgcc tgacctggtc ctcgaccggt ccctgcatgc
    16381 ccaccgcggc gaggtcgccg gcgtcgaagc tggtcagcac ggtgaccgcc accagcttgg
    16441 gccgctgcgg cgccccggcc gcatcggcca ggcgcagcgc ggcggcggcg gcggcgcgca
    16501 tcatcgccgc gccgccggcg gcgtgaaggg tgagcatcat cggcgcgcag ccgagcgcca
    16561 tgcgcaccgc gcccgcgacc gtgttcggga tgtcgtgcag cttgaggtcg aggaacacgg
    16621 gcaggccgtg cgcggcgacc gcgcgcacgc cggccgggcc gtgggcggcg aagaactcga
    16681 ggccgagctt gaccgcgccg acccggccga ccagcgccgc ggcaaggccg ctcgcccgct
    16741 cgaggtcctc ggtgtcgatc gcgcagatga tccggtccgc gcgcccggcg ctggtcatgg
    16801 ccaagcctcc cgtgacgccc ggaccatatc agatcaggaa tgcgccacca aagcgggcct
    16861 cggggtctca cgcgagcccg gcgccgtcgc cggcacggtg cgtgcgcgca tcggtgagcg
    16921 cccgctcagc ggcggcgagc tggcgccggg cgctgcgctc ggcgcgccgg cggcgcccgg
    16981 cgacgatcca gctcgcggtg ccgcccgcca gcacgccgac cgcgagcgag gccagcacca
    17041 gcagatagac cggcaggtcc atgcgcaccg gcaagggtgc cagcgacacg gtcaccggcg
    17101 cccggttggc gagcgcaaag gcgatcgcgg ccagcgcgca ggcgatcgcc agcagcctga
    17161 cgacgagcct caccgcgggg ctctcgcgga cgcgtgcggc gggcgccgca tcaggcccgg
    17221 ttgacgcgct cgcgcagctc cttgcccgac ttgaagtagg ggacgtgctt ctcgggcacg
    17281 cgcaccgccg cgccggagcg cggattgcgc ccgaccctgg acgcccggtc cttgaccgag
    17341 aaggcgccga aaccgcgcag ctcgacccgg tcgccgcgcg ccagcgcagc ggcgatctcg
    17401 tcgaacacgg tcgagacaat gcgctcgacg tcgcggtgaa ataggtgcgg attgtcctgc
    17461 gccagaacct gaatcaattc cgacttggtc atgtcgaagc tgccctcccc gcccgggcac
    17521 caccgaacgc gccgaccctt cgcgccctgc ccgggtcggc aacatgactg agagccgtcc
    17581 tattcggtca cgagagggtg ccaaaccgac accaagccgt caagtttaag tctttcagtt
    17641 aaatgggatt ttccgaacgc agctgagatc agacgatcga ttagccggtc gggatactcg
    17701 atttccacgt cgacctgcgg cagctcggga tcgatgccgc gctgctcctc gagccaggtg
    17761 agcgcctcgg cgacgccgcc gagcccatcg atcaggccga catcgaccgc catggcgccg
    17821 gtgtagaccc ggccgtcggc cagcgcccgc gcccgcgcgc cgtccatctg gcgccgctcg
    17881 gcgaccatgc gcacgaacat gtcgtggacg tcctcgacca gcgccttggt cgccgcgcgc
    17941 gcctcgggcg acatgcggct caacgggttc ggttcggcct tgagcggccc gctcttgatc
    18001 gactcgctct cgatgccgag cttgtccatc aggccgacga agttggtcgc ctccaggatc
    18061 acgccgatcg agccggtgat cgtgctctcg cgggcgacga cgtaatcggc ggcgagcgcg
    18121 gccatgtagc cgcccgacgc ggcgaccccg tccatcaccg ccaccaccgg cttgacctcg
    18181 gcgaccgcgc gcagggcgcg gtacagcgac tcgctcccga aagtggtacc gcccgggctc
    18241 tcgatgcgca cgatcatggc ggcgacgcgg tcgtcgtcga ccaggtcgcg gaccgcctcg
    18301 tcgcggtacg ggtcgctcag gatcacgccg tcgacgtgga acaccgcgat ccgcgccgag
    18361 cgcgagatct tcgacagctg cggcgctcgc ccggccgcca ccgcgaccag ggcgacgatc
    18421 gccaccagcg cgaccagccg ccacagcgtc aggcggcgct tgagccggcg gcggtcgatc
    18481 agggcgtcac tgtcaagggc cataacgggt caggcgtcgg ccgcagtccc cgccggcgcg
    18541 acgcgtcacc ggcgcggggc gggcacggcg cagtctaagg ccagcccggt gcggcggcaa
    18601 tgcgttgggg acagcacgaa gggcggtgac gatcgttcgc gaaaccggcc gctgcggcaa
    18661 gcctactcgg tgtccttggc cttggccgct tccgggtccg cctcggcgcc gggggacgcc
    18721 tcgtcgtcgt cgtcctccgc ctgcgcgtcc tcgccgtcgg cgcgcgcatc ggtggccgag
    18781 tcctcggcct tggccttgat cgccgcgccc aggatgtcgc cgaggctggc gccgctgtcg
    18841 gacgagccgt actcggccat cgcctgcttc tcctcggcga tctcgagcgc cttcaccgac
    18901 agggtcagct tgcgctgggc gcgatcgatc tgggtgacct tggcgtcgag cttgtcgccg
    18961 accgcgaacc ggtccgggcg ctggtcgccg cggtcgcggg cgagatcggc gcggcggatg
    19021 aagccggtgg cgccgccgac ggtcacctcg atgccgttgt cctgcaccgc cgcgacgacg
    19081 ccggtgacca ccgcgccctt cttgaacgtg cgcagcgcgg catcgaacgg gtcctcgacc
    19141 agctgcctga ttcctaagct gatccgttcc ttgtcgacgt cgacctcgag caccttggcc
    19201 ttgaccgtcg cgcccttgcg gaactccttg atcgcctcct cgccggggcg gtcccacgac
    19261 aggtcggaca aatgcaccag gccgtcgatg tccccggcca ggccgacgaa caggccgaac
    19321 tcggtgatgt tcttgatctc gccctcgatc tcggtgccgg ccgggtagcc ggcggcgaac
    19381 gcctgccacg ggttcgcgac gcactgcttg aggccgaggc tgatgcgccg cttctgcggg
    19441 tcgacgtcga gcaccatcac ctcgacctcc tggctggtcg cgacgatctt gcccggatgc
    19501 acgttcttct tggtccagct catctcggag atgtgcacca ggccctcgac gccgggctcg
    19561 agctcgacga aggcgccgta gtcggtgatg ttggtgaccc ggcccttgaa cttggtggcg
    19621 accggatact tggcgtcgac gccctcccac gggtcggcct cgagctgctt catgcccagg
    19681 ctgatgcgct gggtctccgg gttgaagcgg atcacctgca ccttgaccgt ctggccgatg
    19741 ttgagcacct cgctcggatg gttgatccgc cgccaggcga tgtcggtgac gtggagcagg
    19801 ccgtcgacct cgccgaggtc gatgaacgcg ccgtagtcgg tgatgttctt gaccacgcct
    19861 tcgagcacct ggccttcctt gaggctggcg accagctcgg cgcgggcctc ggcgcgggtc
    19921 tcctccatca ccgcgcgccg cgagaccacg atgttgccgc ggcggcggtc catcttgagt
    19981 atctggaacg gctgcttggt gttgagcagg ggggtgatgt cgcgcaccgg gcgcacgtcg
    20041 acctggcttc cgggcaggaa cgccaccgcg ccgccgaggt cgacggtgaa gccacccttg
    20101 acccggccga agatcacgcc ctcgacgcgg tcattggtct cgaacgcgcg ctcgagatgc
    20161 acccaggcct cttcgcggcg cgcgcgctcg cggctgagca cggcttcgcc gcggcggttc
    20221 tcgatccggt cgaggaacac ctcgacccgg tcgccgacct tgggcaccgc cacctcgccg
    20281 gggccggtga actccttgag cgcgacccgg ccctcggcct tcaggccgac gtcgatcacc
    20341 gccatctcgt tgtcgatggc gattacggtc cccaggacga cggtgccttc gaccttatcc
    20401 cgggcctcga ccgattcccg gaacagggcc tcgaagttct catcgatgcc cggcatggcg
    20461 gtgtgcgaag cggcggcgtt gctcatcgtc tcctgttcac tcctggctcg cccaagcgcg
    20521 gcatcacggc ggcgcgcttg gcgcgctcaa cgcgatgccg tgtgcgcgtg ctcactcctg
    20581 ctgacgcgcc agcacataat ccaccgccgc cgcaaacgcg gcgtcggcgt ccaagtgcgt
    20641 cgtatccagc agaagcgcgt ccggcgccgg caccatgggg gcgaccgttc tggcgcgatc
    20701 gcgctcgtcc cgttccgaca actcccgcag gactgcggat cttatactct gagcaccccg
    20761 gccaagcaac tccttatggc ggcgctcgat gcgcgcctcg aggctggcgg tgacgaagat
    20821 cttcacctcc gcgtccggca ggatgacggt gccgatgtcg cggccgtcga gcactgcgcc
    20881 cttggcgccg cccggcgggc agcgcgcgaa ccggcgctgg acctcgacca gcgccgcgcg
    20941 cacgccgggg tgcgcggcga ccaccgacgc cgccgcgccg accgtttcct cgcgcaacgc
    21001 cggcgcggcg atgtcggccg cggtcacggc ctcggcggcg cggcgggcag cggcggcatc
    21061 ggccgggtcg ccgccggcgc tgagcaggcg cagcgcggcg gcgcggtaga gcgcgccggt
    21121 gtcgagatgg gcgaggccga agcgctcggc cagccgcttg gccaaggtgc ccttgccggc
    21181 ggcggccggc ccgtcgatgg cgatgatcac gggctttgtt ccgcagccgc gggggcggac
    21241 cgagcgccgg tcatgggcaa gcacgggacg atggtccgta aagggtcgcc catcgggcgc
    21301 acgacaagcg cccgggcgac gcgccagacg acgccgcccg cgactggccg tgccaccgtg
    21361 cagagcccga tcgtcttgcg gatatccggg cggacttgcg tcggcgcttc gctcaagccc
    21421 accggccgtc cgttcaataa gccttgggca gacccgcctg cttcaggatt gcattggcgg
    21481 tgtgccgtga cttcaacttt ggccggaatc gagaagttgc gctgactgac ggggctgaac
    21541 caaacctcgt ggctgccctt gcccgaccgt atccgacgac agttcgcgtc cctcaggcgc
    21601 cgctccaggt cgcgccggaa gtcggtcatt cggcgggcac gtggaccttt tcctcgcgcc
    21661 gggaggtaac caccaagcag acttcggccg gcccgcggac accgcagttc agctcgagca
    21721 ggtcgggggc cacgagcttg acctcctcga tcagcttctc gagcgtgtcg gcctccgcca
    21781 ccagcccggg gacgtcgtcg ctggtcgcga tccacacgcc ggcctcggcg tcccactcgc
    21841 cacggaccgc gtaaacgcgc cccgccttgg tgtcgtcttg gtctgacatg ccgactccag
    21901 gatatggccg gcggcgacgg cccggcaagc cttgcgcggt ggccggccgg caatcgtgac
    21961 gatcacgacg gctcccgctc ctgcacgatg gcggcgccga ggccgcccat cagctctgcg
    22021 aagccgggga agctggtggc gatcatgccg gcgtcgtcga ccgtgaccgg cgcccgcgcg
    22081 gcgagcccga gcaccaggaa cgccatggcg atgcggtggt ccatatgggt cttgaccacg
    22141 ccgccgccgg gcggcgggcc tgcgcagccg gtgatcgtca ggctgtcggg cccggcctcg
    22201 acggcgacgc cgttggcggc gagcccggcc tcgatcgcgg ccagccggtc gctctccttg
    22261 accctgagct cgcccagccc ctcgagccgg gtgacgccgt cggcgcaggc ggcggcgacg
    22321 gcgaggatcg ggtactcgtc gatcatgcgc gccgcgcgcg ccgccggcac ggtgatgccg
    22381 ctcaggcgcg aggcgcgggc ggtgatgtcg gcgaccggct cgccgcactg ctcgccggtc
    22441 ggggcgatgt cgaggtcggc gcccatctcg cgcaaagtgt cgtagagccc ggtgcgcaac
    22501 ggattgacgc cgaccccgcg gagcgtcacc tcagagcccg gaaccagcag cgccgcgatc
    22561 agcgggaatg ccgccgacga ggggtcgccc ggaacctcga gccgcgtcgc ccggagctcc
    22621 ggctggccgg tcagcgcgac cacgcgcgcg ccgtcagcgt ccgcggcgac cgcgacctcg
    22681 gcgccgaaca ggcgcagcat gcgctcggtg tggtcgcggc tcgccaccgg ctcgatcacc
    22741 gtggtgcgcc cgggcgcgtt gaggccggcg agcaggaccg ccgacttgac ctgcgccgag
    22801 ggcaccggca ggcggtactc gatcggcacc gcatcgccgg tgccgagcac cgtgatcggc
    22861 agccgctcgc cgttggcgca ggcgaaccgg gcgccgaagc gcctgagcgg cgccgtcacc
    22921 cgcgccatcg ggcgggcgcg cagcgactca tcgcccgtga ccaccgcgca caacggatgc
    22981 gtggcgagca tgcccatcag cagccgcgcc gcggtgccgc tgttgccgag gtcgagcacg
    23041 tcggcgggcg ggccgagccc gccgatgccg acgccggcga tgcgccacgc gccgggcgcg
    23101 gtgcgctcga cgcccgcgcc gagcaggcgc agcgcccgcg cggtagcaag cgtgtccgcg
    23161 gcctcgagca ggccgtcgat caccgtcccg ccggcgctga gcgcggccag catcagcgcg
    23221 cggtgcgaga tcgacttgtc gccgggcgca cgcacttggc caacgagcgc gccggccggg
    23281 cgagaggtca agggttgcac gatgcgccgc ctgccggtcc ggtttgcgcg cgacacctag
    23341 cacggttcgg gtcggcgcgc gagccgcggc gcgcggtcgg ttttgctttg acaagcgggg
    23401 agcgagcatg gcaaaggccc ggtcccatgg gaacttctga acaggaggtc cgatcttggc
    23461 caaggctgta ctgggacaga agcacagctg ccgcgaatgt ggcgtgaagt tctacgatct
    23521 cgcccgccag ccgccgacct gtcccgcctg cggcgcaccc atccagtcga ccgacaagcc
    23581 gcggccgcgg cgcgcgcccg cgcccgagcc gcggaaggcg gtgaccgccg tcgccgcgcc
    23641 agaggtcgag gaggtggcgc tcgacgagga tctcgagatc gaaaccgagg atctcgaaaa
    23701 ggagattggc gacgacatcg aggccgagga cgaagaagag ggcggcgacc tgatcgagga
    23761 tgcctcggag ctcggcgagg acgacaccac cgtcgccggc gtggtcggcg aagacgagcg
    23821 ctgagcccgc gcgccgccca gcgaggacca agttaagcct tgcctgggtg cggcgcgctg
    23881 actaggttct gggccggcgc ggcggcggtg tccgccgcac ctcccacggg gccatagctc
    23941 agttgggaga gcgcttgaat ggcattcaag aggtcggggg ttcgactccc cctggctcca
    24001 ccaatcgact cgaggggtcg ggctggcgca gcctggcccc tttcacttcc gaaacggagc
    24061 tcgatcggcg cggcgcttcg atggtcaggg gcgggtcaac cggtggcggg ctaacttgtc
    24121 cgccggcgcc gacggcgatt gtgcgcggca tcggtccagg gcacgctgga tcgttgcgcg
    24181 agcgcaatgc ccaggcccgc gttcgcgtcc gcgcacgctc gtcgtagggg aggtcacaat
    24241 gcaccgtccg gcaatcgtcc tggcctgcgt cctgtgtgcg gcgcaggcac aggcgcaaga
    24301 ggctctcacg cccgagcagg cgaaatgcat cctcgactat atcgacgccg ccaagaccgc
    24361 ccagggcgcg cgcgacatca ggaccgcctg tgtcgactac cggggaaagg acccggagct
    24421 gttcagctgc gtgatcaagc agcaggtcaa ggcgcacagc gacgccggcg cggacgcgat
    24481 tcgcgaggca tgccaggcga tgaagacgca gtagcggcgc gtcgagcccg cgaacggggc
    24541 caacggatgc ctggctaacg tgtccgttcg ccgccggcgg cgagcaggct ctagcgagat
    24601 gcgccgagca ccgcgtcgag gatctgctgg cgggtcggga aggtggccat gctggccatg
    24661 ccggcctcgc cctcgcgcca ggcccagcgc acgacgccgt cccggtcgat caggaaatgg
    24721 ccggcgccgg tcatgccgtg ctgatcgaag atgcgctggt cgacggcatc gagctcgaag
    24781 ccgtccttct tgttgagcgc ccagttcgcc tcgaccggcg acatcggcgc atccagctca
    24841 ccggtcgggt cgaccttgag cgccatgaac tcgtccatgt tgaccagccc gcgcggccag
    24901 tcggtcggcc cctcggtcgc ctgcagccgc cccagcccgt agcgcgcatg ggtgcgccag
    24961 tcggggtcgg cgccgatgtc gatcctcacg gtctggctgc ggtagtagcc gcgcatgcgc
    25021 gcgggctcgg tgttgatcac cgcgaccacc ttgatgccct cgtcgttcag gcgcggggcg
    25081 atgccgttga gcagcgccac ctggcggcgg cagaacgggc agtgcaggcc gcggaacagg
    25141 ccgagcagca cgttgctccg cccgcgatag gcactgaggg taacctcgcg cgccggatcg
    25201 agcccgggca gggtgaactc gggcgcgggg tcgccgaccc cgaccgcgct ctgcacctgt
    25261 tgctgtgcca ttggctcgct cctctcaccg tctgatttcg cgcaactgct ggcacgaccg
    25321 gcgccggtca gtcaaccgct gggtcgggaa cggcagcgag gctacagcct gggctcgcgc
    25381 aggccggcgg cgcgggcgac gaagcgcacg aactcgtccg ccggcagcgg cgcactgacc
    25441 aggcggccct ggatctcgtc gcagccgagg tcgctcagca ggttcgcctg gcgggtggtc
    25501 tcgacccctt cggcgagcac gtgcatgccg aggctgtggc cgaggtggat gatcgcgcgc
    25561 gcgatcgccg aatccagcgg gttcgaggtg atgtcgcgca cgaacgactg gtcgatcttc
    25621 aattttccga ccgggaactt cttcaggtag ttgagcgacg agtagccggt gccgaagtcg
    25681 tcgatcgaca gctcgacgcc gagctcgacc agccggttga gcgtcgcggt cgcctgggcg
    25741 acgtcgggca tcagcgccga ctcggtgatc tcgaggccga gcatgcgcgc ttccacgccg
    25801 gcgccgagca ggctcgcgcg caccttgtcc aggagcgcct ggtcgcggaa ctgcgccgct
    25861 gagacgttga cccagaccgg caccatcaga tggcccttgc gcgcccactc ggcgagctgg
    25921 gtgcagcagc ggtcgagcgc ccactcgccg agcgccagga tcagcccggt cgcctcggca
    25981 tgggcgatga actccgccgg cgcgaccagg ccctgctcgg ggcggtgcca gcgcagcagc
    26041 gcctcgccgc cgatcacgac cccgtcggcg aggcggatcg tgggctggta gtggagcacg
    26101 aactggtcct cggcgagcgc ccggcgcagg ccctcggtga ccgccatgcg ctgctgcgcc
    26161 tggcgggtca tctcgctggc gtaaaactcg agccgccccg gcgccagggc cttggcgcgg
    26221 tgcagcgcga ggtcggcgtt cttcatcagc tcgccgagct cgccggcgtc gtcggggaac
    26281 agggtgatgc cgacgctggc gccggtatgc accggctggc cgtcgatcac caccggcccc
    26341 gacagcgccg ccagcgcgcg ctgggccaat gccgccgcct gctcggcgtc ggcgacctgg
    26401 gtctggatga cggcgaactc gtcgccgccg agccgcgcca cggtgtcgcc gccgcgcgcg
    26461 cagccgcgca gccgcgccgc cacctcgacc agcacccggt cgcccgcggc atggcccaaa
    26521 ttgtcgttga tgtgcttgaa gtggtcgagg tcgagcagca gcaccgcgac cttgcgcccg
    26581 tcgcggcggg cgagcgcgag cgcctgctcg agccggtcct ggaacaggct gcggttgggc
    26641 aggccggtga gcgcgtcgtg gtgggcgagg aagcggatct gctgcgcgtt cgacttgtgc
    26701 tcggtgacgt cgctggcgat gctgatgacg ctgccgtcgg cgagccgccg ctcgttgacc
    26761 gacagccagc gcccgtcctg gcgcgcgagc tcgaccgggc ccgacggcga gcggtgctgg
    26821 tcgagccggc gggcgatcca gtcgtcggcg cagccctcgg cctgcgggta gaggccgcgc
    26881 gcgaccgcgg cgcgcagata ggtctcgtag tcgacccccg gccgccacag ctcgccgagc
    26941 gcggcctcga tcgcccggct cgcccggttg gccagcacca gccggtcgtc ggcgtcgaac
    27001 agcgcgatca gctcgggcac gccgtcgatc gccgtggcca ggcgctcgac cgcggtcgcg
    27061 gcctcggccg aggcggcctg caggccggtc acgtccgagc cgctgaccag caccaggtcg
    27121 tggtcggcgc cgtccagccg gcgcgcctgc accgcgagcc acagcggcgc gccgtcgagg
    27181 cggcggaagc gcagcacgcc tgcggccgcc tcgaccggcg ccgcctcgcc ggcgagcacc
    27241 gcctcggcgg ccgatgccgg cccggcgccg cggtccaaga gcgaggccac cggccggccg
    27301 atcagcgcct cgggcgccgc ggcggcgaac cggcgcgcgc cctcctgatt gacgaacacc
    27361 acccggccgt cggcgtggac cagcaaggtg accggggcat tgtcgaccag gctgcgatag
    27421 cgctcctcgt tgagccggtg ctgcagggca tcgcgcttga gctcggtgat gtcggtccag
    27481 gtgctgaccg tcgagccgtc cgcgagcctg gtctccgaca ccctgagcca gcgcccgagg
    27541 ccggtctcga cctcgaacgc ggcgccgggc gcgcgatggg cggcgatgcg ccggcgcatc
    27601 cagtccttgc tgtcgtgacg cgcggccggc accgcgccgg cctggatcgc gcggtacagg
    27661 agcgcgtcga acggcacgcc ggggcgaacc tggtcggcga cctcggggat cagctcgacg
    27721 aaccggcggt tgaacgcgac cagccggtcc tcgtcgttcc acaccgcgac cgccacgtcg
    27781 gcatgccaca gggcggcggc caggtcggca cgcgtggccg cgtgcggctg ggacgcctgc
    27841 tcgctcacgc ggtccccctt gcgcgacggc atgccattgc gatcggcgcg atcataacag
    27901 cacggtgact cgcgcaacac cgcgccacgg tgagattttg caacactgag tcggacgcgc
    27961 aacaggtggc ggccttgacc gcatcgccgg cggccgcgac agtcgccgcc atgacaccga
    28021 ccgccgccac catccgtccc gcgccatgtg cggccgctac agcctgacct cgcccgccga
    28081 ggcgatggtg gcgctgttcg acgccggccc gctcgccggc ttccagccgc gctacaacgt
    28141 cgcgccgagc caggccatgc cggtggtccg ccacgccgag gcgggcgggc gcgagtgggc
    28201 gtggctgcgc tgggggctga tcccgtcctg ggccaaggac ccggcgattg gtaaccgcat
    28261 gatcaacgcg cgctcggagt cggtggccga caagccggcg ttccgcgccg ccttccgccg
    28321 ccgccgctgc ctggtgccgg ccgacggctt ctacgaatgg cagcagcagg gcggcgtcaa
    28381 gcagccctgg cgcatcacgc tggccgaggg cgggccgttc gctttcgccg ggctgtggga
    28441 gcgctggtca ccgggcgcgg gcgaggcggt cgagacctat accatcctca ccaccgccgc
    28501 caaccgcagc ctggcgccga tccatccgcg catgccggtg atcctcgcgc cggagcatta
    28561 cgcggtctgg ctcggcgacg gccaggcggc cgatccgcgg ctgctcgagc cgctgctcgc
    28621 gccctacccg gatgcgcgtc tg
//
LOCUS       DASWHU010000208        11236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_100831, whole genome shotgun sequence.
ACCESSION   DASWHU010000208 DASWHU010000000
VERSION     DASWHU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100831"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VGB88_07585"
     CDS             <1..995
                     /locus_tag="VGB88_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX9647344.1"
                     /translation="CARERATGFAANEHGVGAGARLTVYCSAECHSSIEKGVTVAGMG
                     RASLRKIAVDDAFAMRADALHAAIRADLDAGLTPAAVVAAIGTTGSSAIDPLAAIADI
                     CRRTGAWLHVDAAYAGSALLLPEKRWMIEGIEHADSFVFNPHKWLFTNFDCSAFFVRD
                     PQHLVRTLAILPEFLRTREGDAVHNFRDWGLQLGRRFRALKLWFVIRSYGVEGLRERI
                     GAHIDMAQRLARAIEASGDFELLAPVPLALLCLRYRPRGIDDEAALDRLNQQLMEAVN
                     DSGSAYLTHTRLGGRFAIRFSIGQTTTEWRHVEAAWRALNEAAHALDAAAVAAS"
     gene            complement(990..3404)
                     /locus_tag="VGB88_07590"
     CDS             complement(990..3404)
                     /locus_tag="VGB88_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9647345.1"
                     /translation="MPLAAIAIVSAGVLAYEVLLMRLFAIIQWHHFAYMVISIALLGY
                     GASGTFLAFTRERLSRAYPLALAANAALFGLTALAAFALVQWLPFNPLEVVWAPAQLW
                     YLGAYYLLFAVPFFCAANCVGLSFASLPARIGAVYRADLTGAGAGALGIVALLFAIPA
                     AGALRAIALAGLGAAVVAALAAPRRIGIPLIAGLALAAGVIALALPPAWTALMPSQFK
                     ALQQALLIPGTRVVAERSSPLGLVSVVESPRVPLRHAPGLSLTSAGEPPAQLGVFVDG
                     EGPSAITAFDGRLAPLSYLGDTTSAAPYRLLDAPRTLVLGAGGGADVLQALYHGARSV
                     DAVELDPAVVDLVRESFADFAGHLYDRPDVHVHIAEARGFVAGARARYDLIQMPLLDS
                     LAAATLGSRGLSESYLYTVEALGDYLDRLAPGGWLAITRWVRVPPRDALKLFATAAEA
                     LRATGAAPAASLAALRSWSTITLLVKNGAITGEDVARLKSFADARSFDLAYAPGMGRA
                     EANRFNVLDAPYFYDAMVALLGPDRARFVERYKFDITPATDDRPYFFDFFRWRALPEL
                     LAIRAQGGAGMLDWGYLILCATLVQALGLALVLVLVPLGFRRTGIAPVPGRGAVALYF
                     LAIGLAFLFVEVASIQRFILFLSHPLYAVAVVLCAFLVFAGLGAGVAPRLAERRGPRA
                     TVAVAVAAIAITALVYLAALPPLFASLMGLGDAAKVAISLALIAPLAFWMGMPFPLAL
                     QVVARRAPALVPWAWGVNGCASVASAVLAALLAMSFGFSAVVLIALALYGLAAASFLR
                     FPAAES"
     gene            complement(3579..4319)
                     /locus_tag="VGB88_07595"
     CDS             complement(3579..4319)
                     /locus_tag="VGB88_07595"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007992649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9647346.1"
                     /translation="MLMTGRIGRSLAAALCLCASVPAAAADDVAEQRAAMIAVIEGHA
                     RGAAAMIGRDHIAAPVLRVMGRVARDRFVPASLHGLAYADRPLPIGSGQTISQPYIVA
                     LMTDLLDPEPGDVVLEIGTGSGYQAAVLAELGARVYTIEIVPELAEAARARLAGLGYD
                     RVATRTGDGYYGWPESAPFDGIVVTAAASHVPPPLVRQLKPGGRMVIPVGGPFSVQYL
                     MLIEVGSDGAVRTRQLLPVAFVPLTGEH"
     gene            complement(4361..5035)
                     /locus_tag="VGB88_07600"
     CDS             complement(4361..5035)
                     /locus_tag="VGB88_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX9647347.1"
                     /translation="MPPSTGGRQRRSASEAWRAAGTALLAALLALAACSQPPVPRDQF
                     YRLAGAPPPQPYQTPPIEGTVVVERLRADGLLSQRPILYTSREWPNRLEQHNYQYWVE
                     SPPEMLSAVLLDYMRAANLATDVAEAGSRRPAGCRLTGTLRRMEQVVTAGAPSLAVIE
                     VELMLERINDRALLLHRTYRAEQPAADLDMAATAQAFDSALAALLARFASDLAAAPLA
                     CPAPIR"
     gene            complement(5059..6060)
                     /locus_tag="VGB88_07605"
     CDS             complement(5059..6060)
                     /locus_tag="VGB88_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9647348.1"
                     /translation="MRPSRVGYFVVGLFVLVMVAGLIGLLTVLGGRGAETERYYTRYA
                     NVAGITFGTPVFYEGFLAGQVEAILPDVADRRTTFKVQISVLAELRIPVDSKVLITQP
                     NLLSGRALSIAAGRKAEFINAGGEIRAGEETGLAALPGLVGGGQELITEARTLIVEAT
                     AAMAQINTWVTDDLGRLSDQYAALPAALQSDMEQLIAEMQATVATATGVVERANLFLS
                     DDNARAVSQTFTNVAELSGQLAETSAELAAVGRDARVVLGQVRELMADNKGDLEGTIV
                     DLRYTIETIAGRIDSITYNMEGTSRNMYEFSRQIRLNPGLLIGGTAQTETAGPGAAQ"
     gene            complement(6080..6904)
                     /locus_tag="VGB88_07610"
     CDS             complement(6080..6904)
                     /locus_tag="VGB88_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9647349.1"
                     /translation="MTESGVPQPPIEPTPAPDRAPVIEVEDLVTHFPGNLEPTLKGVN
                     LTVLDNEIVCIMGGSGSGKSTLLRQIMALDRPTSGRLRILGKDLTNLGQREMYELRKS
                     VGVAFQGGAMFNSMTVGENLRLPLREHARLAEEQMEIMTRMKLAMVNLDARTADKLPS
                     ELSGGMLKRAALARSIMMDPKLLLCDEPSAGLDPAVSAAIDDLILRLREAMSMTVVVV
                     THERTSAFKIADRIIMLDKGEILMTGTLEELRARGSQRVQDLLEGVPEEAELASWI"
     gene            complement(6904..7743)
                     /locus_tag="VGB88_07615"
     CDS             complement(6904..7743)
                     /locus_tag="VGB88_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647350.1"
                     /translation="MADQSPPQAMPTLVEQIGRATVRMVAGCSGTVTEIGYATVLLLQ
                     SLYFLLFGRFRNQPVRASSVVDQMLEIGIRALPIATTLSLIIGITLAMQGIDLLKTFG
                     AQSQVVLAVALSVTREFAPLIMGILVAGRSGSALTARIGTMVISQEIDALRVMGINPV
                     RYLVVPALVAMVTLVPLMTFWADVVGMFGGALYTQLELGMSFQAYADKSLEVLTVGHV
                     MHGIAKSAIFAALIAVTAVVNGFAVSGGAEQVGRATTRTVVVSIAGIIITDMLFIFVL
                     TRS"
     gene            complement(7785..8117)
                     /locus_tag="VGB88_07620"
     CDS             complement(7785..8117)
                     /locus_tag="VGB88_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9647351.1"
                     /translation="METEVREEHGLAVAAFTGDIDFNSSPHARRVLLDLVEKKGDVVV
                     DMSSVDYIDSSGVASLIEAYQSAKTSGARFGLVAVSGAALRVLQLAHLEKVFQIYDSV
                     ADAAARAS"
     gene            complement(8206..8631)
                     /locus_tag="VGB88_07625"
     CDS             complement(8206..8631)
                     /locus_tag="VGB88_07625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647352.1"
                     /translation="MTEAAGTRLVRLRVPAVSDRLRLIRSVVRDAAVISGCSDGCAED
                     IVIAVNEACMNVLEHGYRERQDGDLILDLAVSGGELVVEIVDFAPPVDPQVLRPRPIE
                     ELRPGGLGTHFMHECMDRVVFLTPPEGAGNRLRMVKRIA"
     gene            complement(8628..9932)
                     /locus_tag="VGB88_07630"
     CDS             complement(8628..9932)
                     /locus_tag="VGB88_07630"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9647353.1"
                     /translation="MTRGSPAGLTIARDDHDIGDYLELLAAIGQDFTMSLDIDETLRN
                     TLGRIMAYLDAEAASLFMLTNNDTEIVCRVCVGASDITGLRLKYGQGIVGESIAEEAC
                     LMVRDVRADPRFHRAVDEATGFITRSILCAPMTVKGLHLGAIEVLNKRGGDGLFDERD
                     RQVLRALASSAAMAIRNATLTRSLVEQQRVQRELELVAELQQHLLPKRQPASFPVHGL
                     NLPARTVSGDFYDFFPVGDGRISFTLGDVTGKGIQAALLMAKASSLARCLGKTIHRPG
                     ELLGVINREICDSATRGMFVTMVVGLYEPDTGKVTLANAGHEPPLLIHPDGSRRTFPA
                     EAPPVGISPELFPDARFPEVEAQLGHGSLYVFSDGVTEGRIGGGEQLGADGLETLLRS
                     LSGLPAAERLEVIASKFVHPDTPLHDDLTIMVIEEARAGERP"
     gene            9992..10855
                     /locus_tag="VGB88_07635"
     CDS             9992..10855
                     /locus_tag="VGB88_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9647354.1"
                     /translation="MWRGHGRGLAPAAAALAWLAAGAGGAGEPALEVRLADIRASGLE
                     IHDCSLRFELRNGLDIALKGFAAELILFDAGGRELARKPFEMARVGPGVLRREGLGIK
                     LAADDLPRVTTALELQHQCDVLASAELRLRACESDRESVFDRCLAAIAVAPGGALPLA
                     LAGDSAEEAVDYGVIETPRGARAEAAEKVYFPTLGATVSSITAAIARSRSLSLVTEGL
                     LVVEVAAGGAAAAAGLMAGDVVAEVDQEPVLRPQDLEAALARARAAGHRSLLVLVEGD
                     RGADFRVVRLE"
     gene            10938..>11236
                     /locus_tag="VGB88_07640"
     CDS             10938..>11236
                     /locus_tag="VGB88_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018148969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9647355.1"
                     /translation="MAEGRLDPGGTAASARGEPADAVRAQILRAAQARFARYGFNKTT
                     MAEIAADCAMSAANIYRYFDGKGDIAAVGTGRWLAALETELEGLADDAARAPAERL"
BASE COUNT         1699 a         3939 c         4051 g         1547 t
ORIGIN      
        1 tgtgcgcgcg cgagcgcgcc accggcttcg ccgccaacga gcacggcgtc ggcgccgggg
       61 cgcggctcac cgtctattgc tcggcggaat gccactcctc gatcgagaag ggggtgacgg
      121 tggccgggat ggggcgcgcg agcctgcgca agatcgcggt cgacgacgcg ttcgccatgc
      181 gcgcggacgc gcttcatgcg gcgatccgcg ccgacctcga cgccggcctc acgcccgcgg
      241 cggtggtcgc cgcgatcggc accaccggct cctcagcgat cgacccgctc gccgccatcg
      301 ccgacatctg ccggcgcacc ggcgcctggc tgcacgtcga tgccgcttat gccggcagcg
      361 cgctcttgct gccggagaag cgctggatga tcgagggcat agagcacgcc gacagcttcg
      421 tgttcaaccc gcacaagtgg ctgttcacca acttcgactg ctcggcgttc ttcgtccgcg
      481 accctcagca cctggtgcgc acgctcgcca tcctgcccga gttcctgcgc acccgcgaag
      541 gcgacgcggt gcacaacttc cgcgactggg gcctccagct cggccggcgg ttccgtgcgc
      601 tcaagctgtg gttcgtgatc cgcagctacg gggtggaggg gctgagggag cggatcggcg
      661 cgcatatcga catggcgcag cggctcgcgc gcgccatcga ggcatcgggc gacttcgagc
      721 tgctggcgcc ggtgccgctc gcgctcttgt gcctgcgcta ccggccgcgc gggatcgacg
      781 acgaggcggc gctcgaccgg ctcaaccagc agctgatgga agcggtcaac gactcaggga
      841 gcgcctatct cacccacacc cggctcggcg ggcggttcgc gatccggttc tcgatcggcc
      901 agaccacgac cgagtggcgc catgtcgagg cggcgtggcg ggcgctgaat gaggccgcgc
      961 acgcgctcga tgccgccgcc gtggcggcgt catgactccg cggccggaaa gcgcaggaag
     1021 ctcgccgcgg cgaggccgta aagcgcgagc gcgatcagca ccaccgcgct gaagccgaag
     1081 ctcatcgcca agagcgcggc gagtaccgcg ctcgccaccg aggcgcagcc gttgaccccc
     1141 cacgcccacg gcaccagcgc cggcgcgcgc ctggcgacca cctggagcgc gagcgggaac
     1201 ggcatgccca tccagaacgc gagcggcgcg atgagcgcga gcgaaatcgc caccttggcg
     1261 gcatcgccaa gtcccatcag cgaggcgaac agcggcggca acgcggccag gtagacgagc
     1321 gcggtgatcg cgatcgccgc gaccgcgacc gcgaccgtcg cgcgcggccc gcgccgctcc
     1381 gcgagcctgg gcgcgacgcc ggcgccgagc ccggcgaaca ccaggaacgc gcacagcacc
     1441 accgccaccg cataaagcgg gtggctcagg aacaggatga agcgctggat cgaggcgacc
     1501 tcgacgaaca ggaaggcaag cccgatggcg aggaagtaga gcgcgaccgc cccgcgccct
     1561 ggtaccggcg cgatgccggt acggcggaat ccaagcggca ccagcaccag caccagcgcg
     1621 agacccagcg cctgcaccag cgtcgcgcac aggatcaggt agccccagtc gagcatgccg
     1681 gcgccgccct gggcgcggat cgcgagcagc tcgggcagtg cgcgccagcg gaagaagtcg
     1741 aagaagtacg gccggtcgtc ggtcgccggc gtgatgtcga acttgtagcg ctcaacgaag
     1801 cgcgcccggt cggggccgag cagcgcgacc atcgcgtcgt agaagtacgg cgcatcgagg
     1861 acattgaacc ggttggcctc ggcgcggccc atgcccggcg cgtaggcgag gtcgaacgag
     1921 cgcgcgtccg caaagctctt gagcctggcc acatcctcgc cggtgatcgc gccgttcttg
     1981 accaggagcg tgatcgtgct ccagctgcgc agcgcggcga gcgaggccgc cggcgcggcg
     2041 ccagtcgcgc gcagcgcttc ggccgcggtc gcgaacagct tgagcgcatc gcgcggcggc
     2101 acccgcaccc agcgcgtgat cgcgagccag ccgccggggg cgagccggtc gagatagtcg
     2161 cccagcgcct cgacggtgta gaggtagctt tcgctcaagc cccgcgagcc gagcgtggcc
     2221 gcggcgagcg agtccaacag cggcatctgg atcaggtcgt agcgggcgcg cgcgccggcg
     2281 acgaagccgc gcgcctcggc aatgtgcaca tgcacatcgg gtcggtcgta gaggtggccg
     2341 gcgaaatcgg cgaagctctc gcgcaccagg tcgaccaccg ccgggtcgag ctcgaccgca
     2401 tcgacgctcc gcgccccatg atagagcgct tgcaggacat cggcaccgcc gccggcgccg
     2461 agcaccagcg tgcgcggggc atcgagcagc cggtagggcg cggcggaggt ggtatcgccg
     2521 agataggaca gcggcgcgag ccggccgtcg aaagcggtga tcgcgctcgg gccctcgccg
     2581 tcgacaaaga cgccgagctg cgccggcggc tcgccggcac tcgtcaggct cagcccgggc
     2641 gcatggcgca acggaacgcg cgggctctcg accacgctga ccaggccgag cgggctcgag
     2701 cgctcggcca ccaccctggt gcccggaatc agcaacgcct gctggagggc cttgaactgc
     2761 gacggcatca gcgcggtcca ggcgggcggc agcgcgagcg cgattactcc cgcggcgagc
     2821 gcgagcccgg caatcagcgg tatgccgatc cggcgcggcg cggcaagcgc ggcgacgacc
     2881 gccgcgccga ggccggcgag cgcgatcgcg cgcagcgcgc ccgccgccgg gatcgcgaac
     2941 aacagcgcga cgatgccgag cgcgccggcg ccggcgccgg tgaggtcggc gcgatagacc
     3001 gcgccgatgc gtgcggggag cgacgcgaac gaaagcccga cacagttggc ggcgcagaag
     3061 aacggcaccg cgaacaacag gtaataggcg ccgagatacc acagctgcgc cggcgcccag
     3121 accacctcta gcggattgaa cggcaaccat tggacgagcg cgaaggcggc gagcgcggtg
     3181 agcccgaaca gcgccgcatt ggccgccagc gcgagcggat aggcgcggct caggcgttcg
     3241 cgcgtgaacg ccagaaaggt gccgctggcg ccgtagccga gcagcgcgat gctgatcacc
     3301 atgtaggcga agtgatgcca ctggatgatc gcgaacaggc gcatcagcag cacctcgtag
     3361 gcgagcacgc ccgccgagac gatggcgatc gcggccagcg gcatgggatt gcggactaca
     3421 gcgtcgtccg ctcgcgccga tccggtcccc cctcaccctt ccctctcccc ctgcggctcg
     3481 cggggggaga gggttgcgca gggttggcgc ggcgcatgcc gcgccttacc cggagctggg
     3541 tgaggggggc gggcgcatcc gagcgcgaca tgattcgcct aatgctcgcc cgtcagcggc
     3601 acgaacgcga ccggcagcag ctggcgcgtg cgcacggcgc cgtcgctgcc gacctctatc
     3661 agcatcaggt actggaccga gaacgggccg ccgaccggga tcaccatccg cccgccgggc
     3721 ttgagctggc gcaccagcgg cggcggcaca tggctcgctg ccgcggtgac cacgatgccg
     3781 tcgaacggcg ccgattcggg ccagccgtaa tagccgtcgc cggtgcgggt tgcgacgcgg
     3841 tcgtagccga gcccggcgag gcgggcgcgc gcggcctcgg cgagctcggg aacgatctcg
     3901 atggtgtaga cccgcgcccc cagctcggcg agcaccgccg cctgatagcc tgagccggtg
     3961 ccgatctcga gcaccacgtc gcccggctcg gggtccagca ggtcggtcat cagcgcgacg
     4021 atgtagggct gcgagatggt ctggccggaa ccgatcggca gcggccggtc ggcgtaggcg
     4081 agcccgtgca gcgacgccgg cacgaaccgg tcgcgcgcga cgcgccccat cacgcgcaga
     4141 acgggcgcgg cgatatggtc gcggccgatc attgccgcgg cgccgcgggc gtggccctcg
     4201 atcacggcga tcatcgccgc ccgctgctcg gcgacgtcgt ccgccgccgc cgccgggacc
     4261 gaggcgcaaa ggcaaagcgc ggcggcaagc gatcgtccga tccgtccggt catcagcatc
     4321 tcggcgccga gtgtagcggc tggcggcggc ggccgcgcgc tcagcgtatc ggcgccgggc
     4381 aggcgagcgg cgcggcggcc aggtcgctcg cgaaccgggc gagcaaggcg gcgagggcgc
     4441 tgtcgaacgc ctgtgcggtg gcggccatgt cgaggtcggc cgcgggctgc tcggcacgat
     4501 aggtgcggtg cagcagcagg gcgcggtcgt tgatgcgctc gagcatcagc tcgacctcga
     4561 tcacggcgag cgacggcgcg ccggccgtga ccacctgctc catgcgcctg agcgtgccgg
     4621 tcaggcggca gccggcgggc cggcgcgacc cggcctcggc gacgtcggtg gcgagattgg
     4681 cggcgcgcat gtagtcgaga agcaccgcgc tcagcatctc gggcgggctc tcgacccaat
     4741 attggtaatt gtgctgctcg aggcggttcg gccattcgcg cgaggtgtag aggatcgggc
     4801 gctggctgag caggccgtcg gcgcgcaacc gctcgacgac gacggtgccc tcgatcggcg
     4861 gcgtctggta gggctgcggc ggcggggcgc cggcgaggcg gtagaactgg tcgcgcggca
     4921 ccggcggctg gctgcacgcg gccagcgcca gcagcgcggc cagcagcgcc gtaccggctg
     4981 cccgccacgc ctcgcttgcc gatcgccgct gtcgcccccc agtgctcggc ggcacgccgc
     5041 cagcccccgc tccaatcgtc actgcgccgc gcccggcccc gcggtctcgg tctgcgccgt
     5101 acccccgatc aggagacccg ggttgagccg gatctggcgg ctgaactcat acatgttccg
     5161 gctggtccct tccatgttgt aggtgatcga gtcgatgcgc ccggcgatag tctcgatggt
     5221 gtagcgcagg tcgacgatgg tgccttctaa gtcgcccttg ttgtcggcca tcagctcgcg
     5281 cacctggccg agcacgaccc gcgcgtcgcg gccgaccgcg gcgagctcgg cgctggtctc
     5341 ggcgagctgg ccgctgagct cggcaacgtt ggtgaaggtc tgcgacaccg cccgggcatt
     5401 gtcgtcgctg aggaacaggt tggcgcgctc gaccacgccg gtggcggtgg cgaccgtggc
     5461 ttgcatctcc gcgatcagct gctccatgtc gctctgcagc gccgcgggca gcgccgcgta
     5521 ctggtcgctg aggcggccga gatcgtcggt cacccaggtg ttgatctggg ccatcgccgc
     5581 ggtcgcctcg acgatcaggg tgcgcgcttc ggtgatcagc tcctggccgc cgccgaccag
     5641 cccgggcagc gccgccagcc cggtctcctc gccggcccgg atctcgccgc cggcgttgat
     5701 gaactccgcc ttgcggcccg cggcgatgct gagggcgcgg cccgagagaa gattcggctg
     5761 ggtgatcagc accttgctgt cgaccgggat cctcagctcg gcgagcacgc tgatctggac
     5821 cttgaacgtg gtccgccggt cggccacgtc cggcaagatc gcctcgacct ggccggccag
     5881 gaagccctcg tagaacaccg gggtgccgaa ggtgatgccg gcgacgttgg cgtagcgcgt
     5941 gtagtagcgc tcggtctccg cgccgcggcc gccgagcact gtcagcaggc cgatcaggcc
     6001 ggcgaccatg accaggacga acaggccgac gacgaaatag ccgactcgac tgggacgcat
     6061 ggccgcggct ccccggttgt cagatccagc tcgcgagctc ggcctcttcg gggacgccct
     6121 cgagcaggtc ctggacccgc tgcgagcccc tggcgcgcag ttcctcgagc gtccccgtca
     6181 tcaggatctc gcccttgtcc agcatgatga tgcggtcggc gatcttgaac gcgctcgtgc
     6241 gctcgtgggt gaccaccacc acggtcatgc tcatcgcctc gcgcaggcgc aggatcaggt
     6301 cgtcgatggc ggcggagacc gccgggtcga ggccggccga aggctcgtcg cacagcagca
     6361 gcttggggtc catcatgatc gaccgcgcca gcgccgcccg cttcagcatt ccgcccgaca
     6421 gctcggacgg aagcttgtcg gcggtgcggg catcgaggtt gaccatcgcg agcttcatcc
     6481 gggtcatgat ctccatctgc tcttcggcga gccgcgcatg ctcgcgcagc ggcagcctca
     6541 ggttctcgcc gacggtcatc gagttgaaca tcgccccgcc ctggaaggcg acgccgacgc
     6601 tcttgcgcag ctcgtacatc tcgcgctggc cgagattggt gaggtccttg cccaggatgc
     6661 ggagacggcc gctggtcggg cggtcgagcg ccatgatctg gcgcaacagc gtgctcttgc
     6721 ccgagccgct gccgcccatg atgcacacga tctcgttgtc gagcacggtc aggttcacgc
     6781 ctttgagcgt cggctctagg ttgcccggaa aatgggtgac cagatcctcg acctcgatca
     6841 ccggcgcgcg gtccggtgcc ggcgtcggct cgatcggggg ctggggaacg ccgctctcgg
     6901 tcatcagctt cgggtgagca cgaagatgaa cagcatgtcg gtgatgatga tgccggcgat
     6961 cgacacgacc accgtgcggg tggtggcgcg cccgacctgc tcggcgccgc ccgagaccgc
     7021 gaacccgttg accacggcgg tgaccgcgat cagggcggcg aagatcgcgc tcttggcgat
     7081 gccgtgcatg acgtggccga ccgtgagcac ctcgagggac ttgtcggcgt atgcctgaaa
     7141 cgacatgccg agctcgagct gcgtatacag cgcgccgccg aacatgccga cgacgtcggc
     7201 ccagaaggtc atcaacggta ccagcgtcac catcgccacc agcgccggca ccaccaggta
     7261 gcgaaccgga tttatgccca tcacgcgcag ggcatcgatt tcctggctaa tcaccatcgt
     7321 gccgatgcgt gcggtcagcg ccgagcccga ccggccggcg accaggatgc ccatgatcag
     7381 gggcgcgaac tcgcgcgtga ccgacaacgc gaccgcaagc acgacctggc tttgggcacc
     7441 gaaggtcttg agcaggtcga tcccctgcat ggccagcgtg atgccgatga tcagcgacag
     7501 ggtggtcgcg atgggcagcg cccggatgcc gatctccagc atctggtcga ccaccgagga
     7561 ggcgcggacc ggctggttgc ggaagcggcc gaacagcagg aagtacaggc tttggagcag
     7621 cagcacggtg gcgtagccga tctcggtcac cgtcccggag cagcccgcga ccatgcgcac
     7681 ggtggcgcgg ccgatctgct cgaccagggt cggcatcgcc tgcggtgggg attggtcggc
     7741 catggccgga gctcaagcct gcggacctgc cgcgcgggcc atgctcagct ggcgcgggca
     7801 gccgcatcgg ccaccgaatc gtagatctgg aacaccttct ccagatgggc gagctgaagg
     7861 acgcggagcg cggcgccgct caccgccacc agcccgaagc gggcgccgga ggtcttggcg
     7921 ctctggtagg cctcgatcag gctggcgacc ccggagctgt cgatatagtc gaccgaggac
     7981 atgtcgacca ccacgtcgcc cttcttctcg accaggtcga gcagcacccg ccgggcgtgg
     8041 ggcgaggagt tgaagtcgat gtcgccggtg aaggcggcga cggcgaggcc gtgctcctct
     8101 ctgacttcgg tttccatctc ggcccgccct gagcctccat tcagccgccg gtcgccattg
     8161 tagccgaatt cggccgtcgc ggttcaaccg tggcggcggc gcgcgtcacg cgattcgctt
     8221 gaccatgcgc agccggttgc ccgcaccctc gggcggggtc aggaacacga cgcggtccat
     8281 gcactcgtgc atgaagtggg tgccgagccc gccggggcgc agctcctcga tcgggcgcgg
     8341 ccggagcacc tggggatcga ccgggggcgc gaagtcaacg atctcgacca ccagctcgcc
     8401 acccgagacc gcgaggtcga ggattaggtc gccgtcctgg cgctcgcggt agccgtgctc
     8461 gagcacgttc atgcacgcct cgttgaccgc gatcacgatg tcctcggcgc agccgtcgct
     8521 gcaccccgag atcacggcgg cgtcgcgcac caccgagcgg atcagcctga gccggtcgga
     8581 gaccgccggc acccgcaacc tgaccagtct ggtgcccgca gcctcggtca tggccgctcg
     8641 cccgcgcgcg cctcctcgat caccatgatc gtcaggtcgt cgtgcagcgg cgtgtccgga
     8701 tggacgaact tgctcgcgat cacctcgagc cgctccgcgg ccggcagccc cgacaggctg
     8761 cgcagcagcg tctccaggcc gtccgcgccg agctgctcgc cgccgccgat ccggccctcg
     8821 gtgaccccgt cgctgaacac gtacaggctg ccgtgcccga gctgggcctc gacctccggg
     8881 aagcgcgcat ccgggaacag ctcgggcgag atgccgaccg gcggcgcctc ggccgggaag
     8941 gtgcggcggc tgccgtcggg atggatcagc agcggcggct cgtggccggc attggcgagc
     9001 gtcaccttgc cggtgtcagg ctcgtacagg ccgaccacca tggtcacgaa catgccgcgg
     9061 gtggcgctgt cgcagatctc gcggttgatc acgcccagca gctcgcccgg gcgatggatc
     9121 gtcttgccca ggcaacgcgc caggctcgac gccttggcca tcagcagcgc cgcctggatg
     9181 cccttgccgg tgacgtcgcc gagggtgaac gagatgcgcc cgtcgccgac cgggaagaag
     9241 tcgtagaagt cgcccgagac ggtccgcgcc ggcaggttca ggccgtggac cgggaacgac
     9301 gccggctggc gcttgggcag caggtgctgc tgcagctccg cgaccagctc gagctcgcgc
     9361 tgcacccgct gctgctcgac caggctgcgg gtcagcgtcg cgttgcgaat cgccattgcc
     9421 gccgacgacg ccagcgcccg cagcacctgg cggtcgcgct cgtcgaacag gccgtcgccg
     9481 ccgcgcttgt tgagcacctc aatggcgccg aggtgcaggc ccttgacggt catcggcgcg
     9541 cacaggatcg agcgggtgat gaagccggtc gcctcgtcga cggcgcggtg gaagcgggga
     9601 tcggcgcgca cgtcgcgcac catcaggcag gcctcctcgg cgatgctctc gccgacgatg
     9661 ccctggccgt atttcagcct caggccggtg atgtccgatg cgccgacgca gacccggcag
     9721 acgatctcgg tgtcgttatt ggtgagcatg aacagcgacg ccgcctcggc gtcgagatag
     9781 gccatgatcc gcccgagcgt gttgcgcagc gtctcgtcga tgtcgagcga catggtgaag
     9841 tcctggccga tcgcggccag caactccagg taatcgccga tgtcgtggtc atcgcgcgca
     9901 atggtcaggc ccgccgggct gccccgagtc atggcgccat cataccctct cgccattggc
     9961 aatgaaattc gccacgggta tagctcggct catgtggcgt ggacacgggc gaggcctggc
    10021 cccggcggcc gcggcgctgg cctggctcgc ggccggtgcc ggcggggcgg gcgagcccgc
    10081 gctcgaggtg cgcctggccg acatccgggc ctcggggctg gagatccacg actgcagcct
    10141 gcgcttcgag ctgcgcaacg gcctggacat cgccctgaag ggcttcgccg ccgagctgat
    10201 cctgttcgac gccggcgggc gcgagctggc gcgcaagccg ttcgagatgg cacgcgtcgg
    10261 gccgggcgtc ctgcgccgcg aggggctcgg catcaagctc gcggccgacg acctgccgcg
    10321 cgtcaccacc gcgctcgagc tccagcacca gtgcgacgtg ctggcctcgg cggagctgcg
    10381 cctgcgcgcc tgcgagagcg atcgtgaaag cgtgttcgac cgctgcctgg cggcgatcgc
    10441 ggtggcgccg ggaggcgcgc tgccgctcgc gctcgccggc gattcggccg aggaggcggt
    10501 cgattacggc gtcatcgaga cgccgcgcgg cgcccgcgca gaggcggccg aaaaggtcta
    10561 tttcccgacc ctcggcgcga ccgtgtcgtc gatcaccgcg gcgatcgccc gcagccgctc
    10621 gctgtccctt gtgaccgagg ggctcttggt ggtcgaggtg gctgcgggcg gggcggccgc
    10681 cgccgcgggc ctgatggcgg gcgacgtggt cgccgaggtc gaccaggagc cggtgctgcg
    10741 cccgcaggac ctcgaggcgg cgctggcgcg ggcgcgggcg gccggccatc gctcgctgct
    10801 ggtcctggtc gagggcgacc gcggcgccga cttccgggtc gtgcgcctgg agtgaccggc
    10861 gccccaccgc agcttgatga ttgatcagtg aacattctgt tgattattca gacgccccgc
    10921 cctaagctgc gccggtcatg gccgagggcc ggctggatcc gggcggaacg gcggcgagcg
    10981 cgcgcggcga gcccgccgat gcggtgcggg cgcaaatctt gcgcgccgcc caggcgcgct
    11041 tcgcccgcta cggcttcaac aagacgacga tggcggagat cgccgccgac tgcgccatgt
    11101 cggcggccaa catctaccgc tacttcgacg gcaagggcga tatcgccgcg gtcggcaccg
    11161 gccgctggct ggccgcgctc gagaccgagc tcgaggggct ggcggacgac gccgcgcggg
    11221 cgcccgccga acgcct
//
LOCUS       DASWHU010000209        14471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_478049, whole genome shotgun sequence.
ACCESSION   DASWHU010000209 DASWHU010000000
VERSION     DASWHU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14471
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478049"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..269)
                     /locus_tag="VGB88_07645"
     tRNA            complement(194..269)
                     /locus_tag="VGB88_07645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Thr,seq:cgt)
     gene            complement(329..937)
                     /locus_tag="VGB88_07650"
     CDS             complement(329..937)
                     /locus_tag="VGB88_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647356.1"
                     /translation="MIGFEPASVAPFAFAGAQAPVQPFEVPFGRDDDRDTISGRLAAT
                     LDRLFRHLHRHPAHRAKAVAMAAQSLSPLVDQAIRITAGSFRLQIASLSVDSNGRISA
                     QTLAVEAQLLRIEATTPEASIVLDIAGARISETSAEIGPGGTLRQDSRTLDLSALGLS
                     ARTGDTTLDALQVIASLAQSRLALEAYRAGEASPLQRLLGEA"
     gene            complement(1043..2401)
                     /locus_tag="VGB88_07655"
     CDS             complement(1043..2401)
                     /locus_tag="VGB88_07655"
                     /inference="COORDINATES: protein motif:HMM:NF018875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX9647357.1"
                     /translation="MTRLLAFLAVVAVLSAAAAWIADRPGAVSLRWQGWQVDTSVAVL
                     VLAIAVLGALVAAAYNLYRWLVVSPRTIRRFRTERRRQRGYRALTRGLVSAAAGDAPG
                     ARAAARDAEALLGEPPLTLLLTAQAAQLDGDSRAAGVAFRRMLEHDQTEFLGLRGLLV
                     QAVRKGDAATALTLARRAYQINPDAAWVLDNLIQLESAAGNWDGAETAVASAVKARRL
                     AHAEGARKRALFDFERARAADAAGDSAGALGLAKKALAKEPDLVPAIALAARLMIARG
                     QARGARRLIERSWPACAHPDLARAYAAAAPDGGALAKVKALERLVDACPGEVEGHVAL
                     AGAAIEAGLWGTARNHLGRAEALRESAAVFRLRAELEEAETGDVERARSWWRKAAEAP
                     PDPVWLCRRCGAPAGTWTLICAACGAVDSLAWRTPPVAAVVADPLLAPAPAAALRIER
                     QS"
     gene            complement(2398..4071)
                     /locus_tag="VGB88_07660"
     CDS             complement(2398..4071)
                     /locus_tag="VGB88_07660"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9647358.1"
                     /translation="MRILITRPRADSEALAARLAAAGIDGVVAPMLEVLLREGPPLDL
                     AGVQAVLATSANGARAFAARSDRRDLPLLAVGEATAAAARAAGFTDVLAAEGDSEQLA
                     ELARDRLEAGAGTVLHAAGAHTAGDLAGALAAAGFDCRREVLYEAVAAEALPGEAARA
                     LADGTLDGVALFSPRTARTFVALARAAGHQAALAGLTAYCLSEAVAGAAERALWRRVV
                     VAVRANADSLIELIRADLPARQAPPGVAARAGGGWRAPVLAAAVAAVVSVLVVLGLAS
                     WLAPAPAPAEAPPDPRIGSLEQRLDDLARRVDSRFAALPPAAAPAAVEALAGDLAARE
                     RALRADLARSRDAAADLAQRLDALETRIAALSDRMAQADTGGQARRAGALLIGLGQLR
                     AAIADARPYQGELDALSTLAAGESALQPALAGLAAAAPAGVETEAELGARFPAVAVAA
                     LKEGAGTADDGWLGHALAELRSLVTIRRVGADVAGTDADAVLARAQAHIEAGDLAAAV
                     AALAALRPVPAPLAAWLAEARALLDARAALDALTVLIVAHAEASEPPAP"
     gene            complement(4080..5015)
                     /gene="hemC"
                     /locus_tag="VGB88_07665"
     CDS             complement(4080..5015)
                     /gene="hemC"
                     /locus_tag="VGB88_07665"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9647359.1"
                     /translation="MTNRALRIGTRGSPLALAQTALVREALVAAEPGLGAAGALDVQL
                     IRTTGDIAGDRPLAEIGGKGLFTKEIDEALAAGRIDLAVHSMKDVPTWLPGGQVIGAY
                     LAREDPRDAWIAREGLGLGDLAAGSVVGTSSLRRAAQVRYRRPDLRVVPFSGNVGTRL
                     DKLARGEADATLLALAGLRRLGREDVATAILDAEEMLPAIAQGAIGIACRADDARTRA
                     LLACIDHPATARAIAAERALLAGLDGSCRTPIAGLAEPAPGGRLVLRGLLAKPDGSAV
                     VADRAEAPATDAEALGAALAERLLARAGAGFLAAE"
     gene            5114..6157
                     /gene="tsaD"
                     /locus_tag="VGB88_07670"
     CDS             5114..6157
                     /gene="tsaD"
                     /locus_tag="VGB88_07670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9647360.1"
                     /translation="MLVLGIETSCDETAAAVVGDDRTIHADLVLSQLDDHAPYGGVVP
                     EIAARSHVDHLDALIARALDEAGVGFADLGGVAATAGPGLIGGVMVGLTTAKAICAVR
                     ALPLVAVNHLEAHALSARLVADIAFPYLLLLISGGHCQLLVVEGVGRYRRLGTTVDDA
                     LGEAFDKAARLLGLGYPGGPEIERAAEAGNGARYELPRPMLGRPGCDFSFSGLKTALG
                     RLVAGLAGGAPDASATADLAASFQAAVADVVCDRVRNAAARFHADHGPGGVLVVAGGV
                     AANRFLRKRLAEAAARLGLELVVPPARLCTDNGAMVAWAGIERLALGLADGLDAPARA
                     RWPLDERRVPVEA"
     gene            6159..7154
                     /locus_tag="VGB88_07675"
     CDS             6159..7154
                     /locus_tag="VGB88_07675"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9647361.1"
                     /translation="MARLSVLGAGAWGTALAVHAARQGHDVILWVRREELATRMVAAR
                     ENADYLPGVALGPKVTITAAVAEAAAPVMLAAVPAQFLRAALARAAGAVAPDAAVVIC
                     AKGIERASGALMSQVVAEALPGRGIAVLSGPTFAGEVARTLPTAVTLACADSLLGREL
                     VALLGAATFRPYLTDDVIGAQAGGATKNVLAIACGIAAGRRLGENARAALITRGLAEI
                     GRLSASLGGRAETLMGLSGVGDVVLSCTSASSRNYALGQALGEGGALAALVGGRRSVA
                     EGVDTAAAVATLSARLGVDMPIAAAVNAVLHEGAELDATIARVLARPFRPEVGAG"
     gene            complement(7165..7992)
                     /gene="dapD"
                     /locus_tag="VGB88_07680"
     CDS             complement(7165..7992)
                     /gene="dapD"
                     /locus_tag="VGB88_07680"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9647362.1"
                     /translation="MTADALETAVERAWAARERIGPATGGEAREAVEAALDGLDRGSL
                     RVAEQRDGAWHVHQWLKKAVLLSFRLNDVAPISGGPGAASWYDKVPSKFAGWGEAEFR
                     AAGFRAVPGAIVRRSAYIAPGAVLMPCFVNLGARIGAGTMVDTWATVGSCAQIGANCH
                     ISGGAGIGGVLEPLQAAPVIVEDGCFIGARSELAEGVVVEQGAVLAMGVYIGASTKIV
                     DRASGQVSFGRVPAYAVVVPGSLPGPAPGPALYCAVIVKRADAATRAKTSINELLRD"
     gene            complement(7989..8735)
                     /locus_tag="VGB88_07685"
     CDS             complement(7989..8735)
                     /locus_tag="VGB88_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX9647363.1"
                     /translation="MVAPGPVPEPDLSRANTLDDWVFDLDNTLYAADCDLFAQIDDRM
                     GQYISSLLGVDRAAARAIQKGYYHRHGTTLRGLMLDHGVDPDHYLDFVHDIDLSPLAP
                     APALARALDRLAGRKVVFTNGSAAHAARVLDRLGIADRFAAVFDIAAAGWLPKPHPGA
                     YRRMLDVCRVSARRAALVEDSLANLAPAAALGMTTVWVRHPRARGDRADPVAGADGEA
                     PAHVDHVTDDLAAWLAAVAEAGGAVAATEP"
     gene            8867..10597
                     /locus_tag="VGB88_07690"
     CDS             8867..10597
                     /locus_tag="VGB88_07690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX9647364.1"
                     /translation="MPATSTLIPPSGGGHAGAPEPVRSPASEQFLAAYPGPALLSDGA
                     GRVVMANAQAGNLQSAIEEGMVPDLALALSRALASGESQTATAILPAHLGGSGLNLTL
                     VAGGVGLVGAKWVLVLGSDASLEINLRNALVESRQRYKDLVDCSSDFAWETGKDGRFV
                     FVSPAGVLGYHPDELIGRAARDFVSADFEPPEELPFDGARRMEDVVVWFRAAGGGNAC
                     LTVSSVPLFGPDGAWAGARGVGRDITAGRAKDLLLAVARRREQVLDAVARATRIEVEA
                     PAMLIAASRAVAEAMELSACWVLRLDASRQPYVAASWPPRQPPPASVMSRLAEVVARA
                     AADAHHALEVDGALLVPAQHAQCVNGAIAALQGPGDWGIEDRDLMSAVAGQVGILIEQ
                     ASIHEQLQVMSRTDPLTGLLNRRAFLDEAGRRLAHATRTGRGAGVLYIDLNNFKAIND
                     AFGHGAGDRILRTVTRVIADTGRGGDVASRYGGDEFVIWLDETNLAGANARAEKLIAL
                     IDKRTDMPEVPGRRFGLAIGVAAFDPASGEQLASLLERADRAMYEAKRARDGFGLEIA
                     PAFAREAGTA"
     gene            10594..11910
                     /locus_tag="VGB88_07695"
     CDS             10594..11910
                     /locus_tag="VGB88_07695"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9647365.1"
                     /translation="MSDLVRQDHRAPPAGGEARSESRTALASGAELSYQQARVLARDR
                     DVAVRRRLAAREDVAPEILYYLAEDADAEVRRAVATNPATPRHADLKLARDANDDVRI
                     ELAHKIARLVPGLPAAERDALYRLTIEALTTLARDALPHVRRVIAEEIKAMDAVPREV
                     VQRLARDLEVMVAAPVLEFSPLLNDADLLEIIHSAPVRGALSAISRRRALAAPVCDAI
                     AATGEVAGVAELLANASAQIREETLDKLIDQAHAHPEWQRPLAVRPQLSESAARRLMR
                     FVSRALLTELERANRLAPAVAAALAERVEARLETGGAECEDAAAAEDRSEVAEQMRAA
                     DRRGELDQAAIGRLIRDGKLEHLVQALAIRAEVTSLGVRRIIRSANATALVALAWKAG
                     LDMATATGLQARIANIAPELLIQPAADGGYPIEADELEWRLYLFTA"
     gene            complement(12179..13111)
                     /gene="argB"
                     /locus_tag="VGB88_07700"
     CDS             complement(12179..13111)
                     /gene="argB"
                     /locus_tag="VGB88_07700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9647366.1"
                     /translation="MSETAAPSLEERLHTAATLSEALPYMRRFAGKTFVIKFGGHAMG
                     EDSLAESFARDVVLLKQVGINPVVVHGGGPQIGKMLERLKISSSFVHGLRVTDRATVE
                     IVEMVLAGSINKQIASAINRAGGTALGLCGKDGHLIRARRLEHKMRDPDSNLERVVPL
                     GFVGEPESINVEVLEMLARSGCVPVIAPIGFGADGETYNINADTAAGAIAAALGATKL
                     LMLTDVAGVLGRDGALIATLTPEAARACIDDGTVSGGMVPKIETCIAAIEAGTEAAHI
                     LDGRIPHVLLLEVFTAHGVGTMIARSGCAPTHAA"
     gene            complement(13151..13807)
                     /gene="yihA"
                     /locus_tag="VGB88_07705"
     CDS             complement(13151..13807)
                     /gene="yihA"
                     /locus_tag="VGB88_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX9647367.1"
                     /translation="MAAACEPEAAAIEAARRLFAQPCRFVAGAVRLDQLPAAELPEAA
                     FAGRSNVGKSSLINALVGQRALARISATPGRTRQINLFDLGRRLMLADLPGYGYARAP
                     KREVAAWTELVRDYLRGRASLRRVCLLVDARHGLMAADRPVAAMLDAAGVAYQIVLTK
                     ADRLTAAVAAERIAAIAATLARHPAAHPEVIATSARSGQGIEALRAALAALAAPAPVG
                     "
     gene            complement(13812..>14471)
                     /gene="yidC"
                     /locus_tag="VGB88_07710"
     CDS             complement(13812..>14471)
                     /gene="yidC"
                     /locus_tag="VGB88_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9647368.1"
                     /translation="PLFYVIHTFSRWLGNFGLAILLLTVLIKLVFYPLANKSYTAMSK
                     LKKLQPEMMKLREQCGDDKQKLNAEVMALYKREKANPLAGCLPIVVQIPVFFALYKVL
                     FVTIEMRHAPFYGWIHDLSVPDPTNLFTVFGLLPLTLPDFLSIGVWPLLMGVTMFLQQ
                     KLNPTPPDPTQAKIMMLLPVMFTFLFARFPAGLVIYWTWNNLLSIAQQWVIMRRMGVR
                     A"
BASE COUNT         1883 a         5328 c         5462 g         1798 t
ORIGIN      
        1 gctatgcccc gttatatcac ttgccgctca agctgtgagg cgtaggaggt ttccccaagc
       61 atatttacgc gtcaactttc ttacgtggcc gaaacgagtt gggacggcga tgtctccgca
      121 gcgaccgctt tgctggagcc ccggtgctgt gaaggcgcat tgtcaccaac tcacgtcgtt
      181 cgtgcacgga cactggtgcc gcaggaggga ctcgaacccc cgacccgcgc attacgaatg
      241 cgctgctcta ccaactgagc tactgcggcg cagggcgcag cgaccttacg cgcgcgctgg
      301 tccgcgcgca agcggaggcg acggccgctc aggcctcgcc gagcaggcgc tggagcgggc
      361 tcgcctcgcc ggcacgatag gcctcgagcg cgagccggga ctgggcgagg ctcgcgatca
      421 cctgaagcgc gtcgagcgtc gtgtcgccgg tgcgtgcgct caggccgagc gcggacaagt
      481 caagggtccg gctgtcctgg cggagcgtgc cgccgggccc aatctcggcg ctggtctcgg
      541 agatgcgcgc gccggcgatg tcgagcacga tcgaggcctc gggcgtggtc gcctcgatgc
      601 ggagcagctg tgcctcgacc gccagggtct gcgccgagat gcggccgttc gagtcgaccg
      661 acaggctcgc gatctgcagc cggaagctgc cggcggtgat gcggatcgcc tgatcgacca
      721 gcggcgacag cgactgcgcc gccattgcca ccgccttggc acgatgggcc gggtggcggt
      781 gcaggtggcg gaacagccgg tcgagcgtgg cggcgagtcg gcctgagatg gtgtcgcggt
      841 cgtcgtcgcg gccgaacggc acctcgaacg gctggaccgg ggcctgggcg ccggcaaagg
      901 cgaacggcgc gacggatgcg ggctcgaacc cgatcatggc actcctccat tctggaggcg
      961 gggcgtgcgc gttctgcgcc ccgcaccgct caccatatgg cgccaccggc gcgcgccggc
     1021 cagtttggat cagcactcct gatcagcttt ggcgctcgat ccgcagcgcg gcggccggcg
     1081 cgggcgcaag gagcggatcg gctaccacgg cggccaccgg cggggtgcgc caggcgagcg
     1141 aatcgaccgc gccgcaggcg gcacagatca gcgtccaggt accggccggc gcgccgcagc
     1201 gccggcacag ccagaccgga tcgggcggcg cctcggcggc cttgcgccac cagctgcgcg
     1261 cgcgctcgac atcgccggtc tcggcctcct cgagctcggc gcgcaggcgg aagaccgcgg
     1321 cgctctcgcg cagcgcctcg gcccggccca ggtggttgcg cgcggtgccc cacagccccg
     1381 cctcgatcgc cgcgccggca agcgcgacgt ggccctcgac ctcgcccggg caggcatcga
     1441 ccagccgctc gagcgccttg accttggcca acgcgccgcc gtccggcgcc gccgcggcat
     1501 aggcgcgggc gaggtcggga tgggcgcacg ccggccagct ccgctcgatc agccggcgcg
     1561 cgccgcgcgc ctggccgcgc gcaatcatca gccgcgccgc gagcgcgatc gccggcacca
     1621 ggtcgggctc cttggcgagc gccttctttg ccaagccgag cgcgcccgcc gagtcgcccg
     1681 cagcgtcggc ggcgcgcgcg cgctcgaagt cgaacagcgc gcgcttgcgc gcaccctccg
     1741 cgtgcgcgag ccggcgcgcc ttgaccgcgc tcgccaccgc cgtctcggcg ccgtcccagt
     1801 tgccggccgc gctttcgagc tggatcaggt tgtcgagcac ccaggcggcg tccgggttga
     1861 tctggtaggc gcgccgggca agggtgagcg cggtcgcggc atcgcccttg cgcaccgcct
     1921 ggaccagcag cccgcgcagg cccaggaact cggtctggtc gtgctcgagc atgcgccgga
     1981 aggcgacgcc ggcggcgcgc gaatcgccgt cgagctgcgc cgcctgggcg gtgagcaaga
     2041 gcgtcagcgg cggctcgccg agcagcgcct cggcgtcgcg cgccgccgcg cgcgcccccg
     2101 gcgcgtcgcc cgccgccgcc gaaaccaggc cacgggtgag cgcgcgatag ccgcgctggc
     2161 gccgccgctc ggtgcggaag cggcgaatgg ttcgcgggct aaccacgagc cagcgataga
     2221 gattgtaggc ggcggcgacc agcgcgccga gcacggcgat tgcgagcacc agcaccgcga
     2281 ccgaggtgtc gacctgccag ccctgccagc gcaagctgac cgcccccggc cggtcggcga
     2341 tccacgccgc ggcggccgac agcaccgcga ccaccgccag gaaggcgagc aggcgggtca
     2401 cggcgcgggc ggttccgagg cctcggcgtg ggcgacgatg agcacggtga gcgcatcgag
     2461 cgcggcgcgc gcgtcgagca gtgcgcgcgc ctcggcgagc catgccgcga gcggcgcggg
     2521 caccggccgg agcgcggcca gcgcggccac cgcagcggca agatcgcccg cctcgatgtg
     2581 cgcctgcgcg cgggcgagga cggcgtccgc gtccgtgccc gcgacgtcgg cgccgacgcg
     2641 gcggatggtg accaggctgc gcagctcggc gagcgcatgg ccgagccagc cgtcgtcggc
     2701 ggtacctgcg ccctccttga gcgccgccac cgcgaccgcg gggaagcggg cgccgagctc
     2761 ggcctcggtc tcgaccccgg cgggcgccgc tgccgccagg ccggcgagtg ccggctgcaa
     2821 cgcgctctcg ccggcggcga gcgtgctgag cgcatcgagc tcgccctgat aggggcgcgc
     2881 atcggcgatg gcggcgcgca gctggccgag cccgatcagc agcgcgccgg cacgccgcgc
     2941 ctggccgccg gtgtcggcct gcgccatgcg gtcggagagc gccgcgatcc gggtctcgag
     3001 cgcatcgagc cgctgcgcga ggtccgccgc cgcgtcgcgg ctgcgagcga ggtccgcgcg
     3061 cagcgcccgc tcgcgcgccg ccaggtcgcc ggcgagcgcc tcgaccgcgg ccggcgccgc
     3121 cgccggcggc agcgccgcga agcgcgagtc gacgcgccgg gcgaggtcgt cgagccgctg
     3181 ctccaacgag ccgatgcgcg ggtcgggcgg cgcctccgcc ggcgccggcg cgggcgcaag
     3241 ccacgatgcg agccccagca ccaccagcac gctcaccacc gcagccacgg ccgctgccag
     3301 caccggggcg cgccagccgc cgcccgcccg cgcggcgacg cccggcggcg cttgccgggc
     3361 tggcaggtcg gcgcggatca gctcgatcag cgaatccgca ttcgcccgca cggcaaccac
     3421 gacccgccgc cacagcgcgc gctcggcggc gccggcgacg gcctcgctca ggcagtaggc
     3481 ggtgagcccg gcgagcgcgg cctggtggcc ggcggcgcgc gccagggcga cgaaggtgcg
     3541 cgcggtgcgc ggcgagaaca gggcgacgcc gtcgagcgtg ccgtcggcga gggcgcgggc
     3601 cgcctcgccg ggcaaggcct cggccgcgac cgcctcgtac agcacctcgc gccggcagtc
     3661 gaagccggcg gcggcgagcg cgcccgcgag gtcgcccgcg gtgtgcgcgc cggcggcatg
     3721 gagcaccgtg cccgcacccg cctcgagccg gtcgcgggcg agttcggcga gctgctcgga
     3781 atcgccctcc gcggcgagca cgtcggtgaa cccggccgcg cgcgccgccg ccgccgtcgc
     3841 ctcgccgacc gcgagcagcg gcaggtcgcg gcggtcgctg cgggcggcga aggcgcgggc
     3901 gccgttggcg ctggttgcga gcaccgcctg gacgccggcg aggtcgagcg gcgggccctc
     3961 gcgcagcagc acctcgagca tcggcgcgac cacgccgtcg atgccggcgg cggcgagccg
     4021 cgcggccagg gcctcgctgt cggcgcgcgg ccgggtgatg aggatgcgca tcggcgcgcc
     4081 tactccgcgg ccaggaagcc tgcgcccgcg cgcgccagca gccgctcggc gagcgcagcg
     4141 ccgagcgcct cggcgtcggt cgcgggcgcc tcggcgcggt cggcgaccac ggcgctgccg
     4201 tccggcttcg ccaacagccc gcgcagcacc agccggccgc ccggtgcggg ctcggcgagg
     4261 cctgcgatcg gggtccggca ggagccgtcg aggccggcaa gcagcgcccg ctcggcggcg
     4321 atcgcgcgcg cggtggccgg atggtcgatg caggcgagca gggcgcgcgt gcgcgcgtcg
     4381 tccgcgcggc aggcgatgcc gatcgcgccc tgggcgatcg cgggcagcat ctcctcggca
     4441 tcgaggatcg cggtcgccac gtcctcgcgg cccagccggc gcagcccggc gagcgccagc
     4501 aaggtggcgt cggcctcgcc gcgcgcgagc ttgtcgagcc gggtgccgac attgccgctg
     4561 aacggcacca ccctgaggtc ggggcggcgg tagcggacct gcgccgcgcg cctgagcgag
     4621 gaggtgccga ccacgctgcc cgcggcgagg tcgccaaggc cgaggccttc gcgcgcgatc
     4681 caggcgtcgc gcgggtcctc gcgggcaagg taggcgccga tcacctggcc gcccggcagc
     4741 caggtcggca cgtccttcat cgagtgcacg gccaagtcga tgcggcccgc ggcgagcgcc
     4801 tcgtcgatct ccttggtgaa caggcccttg ccgccgatct cggccagcgg ccggtcgccg
     4861 gcaatgtcgc cggtggtgcg gatgagctgg acatcgagcg ccccggccgc gccgaggccg
     4921 ggctcggccg cgaccagagc ctcgcgcacc agcgcggtct gggcgagcgc gagcgggctg
     4981 ccgcgggtgc cgatgcggag tgcgcggttg gtcatacctg ctgtggcggt ctatacaggg
     5041 cgtccttata acccagccgg ggtgcgcggc gaaccgcgga atggcgcgcg cggcgccccg
     5101 ccggacggtg gccatgctgg tgctcggcat cgagacgagc tgcgacgaga ctgcggcggc
     5161 ggtggtcggc gacgaccgca cgattcacgc cgacctggtg ctgtcgcagc tcgacgacca
     5221 cgcgccgtat ggcggggtgg tgcccgagat cgcggcgcgc agccatgtcg atcatctcga
     5281 tgcgctgatc gcgcgcgcgc tggatgaggc cggcgtcggc ttcgccgacc tcggcggcgt
     5341 cgccgcgacc gcgggcccgg gcctgatcgg cggggtgatg gtcgggctga ccaccgccaa
     5401 ggcgatctgt gccgtgcgcg ccctgccgct ggtcgcagtc aaccacctgg aggcgcacgc
     5461 gctgtcggcg cgcctggtcg ccgacatcgc cttcccctac ctgctgctgc tgatctcggg
     5521 cggccactgc cagctcctgg tggtcgaggg tgtcggccgc taccggcgtc tcggcaccac
     5581 cgtcgacgac gccctcggcg aggcgttcga caaggccgcg cgcctgctcg ggctcggcta
     5641 tccgggcggg ccggagatcg agcgcgcggc cgaagccggt aacggcgcgc gctacgagct
     5701 gccgcggccg atgctggggc ggcccggatg cgacttctcg ttctcgggcc tcaagaccgc
     5761 gctcggccgg ctggtggcgg gactggccgg cggtgcgccg gacgcgagcg cgacggccga
     5821 cctcgccgcc tcgttccagg ccgcggtcgc cgacgtggtg tgcgaccggg tgcgcaacgc
     5881 cgccgcgcgc ttccacgccg accatggccc gggcggcgtg ctggtggtcg cgggcggggt
     5941 cgcggctaac cggttcctgc gcaagcgcct cgccgaggcg gcggcgcggc tcgggcttga
     6001 gctggtggta ccgccggcca ggctgtgcac cgacaacggc gcgatggtcg cctgggccgg
     6061 catcgagcgg ctcgcgctcg gcctcgccga tgggctcgat gcgccggcgc gcgcgcgctg
     6121 gccgctcgac gagcggcgcg tgccggtcga ggcctgagat ggcgcggctt tcggtgctcg
     6181 gcgccggcgc ctggggaacc gcgctcgcgg tccatgccgc gcgccagggc cacgacgtca
     6241 tcctgtgggt gcggcgcgag gagctggcga ccaggatggt ggcggcgcgc gagaacgccg
     6301 actacctgcc gggggtggcg ctcggcccca aggtcacgat caccgccgcc gtcgccgagg
     6361 cggcggcgcc ggtgatgctc gccgcggtgc cggcacagtt cctgcgcgcg gcgctcgccc
     6421 gcgccgcggg cgcggtcgcg cccgatgcgg cggtggtgat ctgcgccaag ggcatcgagc
     6481 gggcgagcgg cgcgctgatg agccaggtgg tggccgaggc gctgcccggg cgcggcatcg
     6541 cggtgctgtc cgggccgacc ttcgccggcg aggtggcgcg cacgttgccg accgcggtca
     6601 cgctggcctg cgcggactcg ctgctcggcc gggagctggt cgccctcctc ggcgccgcga
     6661 cctttcgccc gtacctgacc gacgacgtga tcggcgccca ggccggcggc gcgaccaaga
     6721 acgtgctcgc catcgcctgc ggcatcgccg ccgggcgccg cctcggcgag aacgcgcgcg
     6781 ccgcgctgat cacccgcggc ctggccgaga tcggccgcct cagcgcaagc ctgggcgggc
     6841 gcgcggagac gctgatgggg ctgtcggggg tgggcgacgt ggtgctgagc tgcaccagcg
     6901 cctcgtcgcg caactacgcg cttggccagg cgctcggcga gggcggcgcg ctcgcggcgc
     6961 tggtcggcgg ccggcgctcg gtggccgagg gagtcgacac agcggccgcc gtcgccaccc
     7021 tgagcgcgcg cctcggcgtc gatatgccga tcgcggcggc ggtgaacgcc gtgctccacg
     7081 agggcgccga gctcgacgcc actatcgccc gggtgctggc gcgcccgttc cggcccgagg
     7141 tcggcgccgg ctgaggacgc gcgctcaatc gcgcagcagc tcgttgatcg aggtcttggc
     7201 gcgggtggcc gcgtccgccc gcttgacgat caccgcgcag tagagcgcgg ggcccggcgc
     7261 cgggcccggc agcgagccgg gcaccaccac cgcataggcc ggcacccggc caaagctgac
     7321 ctggccgctg gcgcggtcga cgatcttggt cgaggcgccg atgtagacgc ccatcgccag
     7381 caccgcgccc tgctccacca ccacaccttc ggcgagctcg ctgcgcgcgc cgatgaagca
     7441 gccgtcctcg acgatcaccg gcgccgcctg cagcggctcc agcaccccgc cgatgccggc
     7501 gccgcccgag atgtggcagt tggccccgat ctgcgcgcaa gagcccaccg tcgcccaggt
     7561 gtcgaccatg gtgccggcgc cgatgcgcgc gcccaggttg acgaagcacg gcatcagcac
     7621 cgcgccgggc gcgatatagg ccgagcggcg gacgatcgcg ccgggcaccg cgcggaagcc
     7681 ggcggcgcgg aactcggcct cgccccagcc ggcgaacttg gacggcacct tgtcgtacca
     7741 cgacgctgcc ccggggccgc ccgagatcgg cgccacgtcg ttgagccgga acgacagcag
     7801 cacggccttt ttcaaccact ggtgcacatg ccaggcgccg tcccgctgct cggcgacgcg
     7861 caagctgccg cggtcgagac cgtcgagcgc cgcctcgacc gcctcgcgcg cctcgccccc
     7921 ggtcgcgggc ccgatccgct cgcgcgccgc ccacgcccgc tcgaccgccg tctcgagcgc
     7981 gtccgccgtc atggctcggt cgcggcgacc gccccgcccg cctcggcgac cgcggcgagc
     8041 caggcggcga ggtcgtcggt gacgtggtcg acatgggcgg gcgcctcgcc gtccgcgccc
     8101 gccaccgggt cggcgcggtc cccgcgtgcg cggggatggc gcacccacac cgtggtcatg
     8161 ccgagcgccg ccgcgggcgc gaggttggcg aggctgtcct cgaccagcgc cgcgcgccgc
     8221 gccgacaccc ggcacacgtc cagcatgcgc cggtaggcgc ccggatgcgg cttcggcagc
     8281 cagccggcgg cggcgatgtc gaacaccgca gcgaagcggt cggcgatgcc cagccggtcg
     8341 agcacccgcg ccgcgtgcgc tgccgagccg ttggtgaaca cgaccttgcg cccggcgagc
     8401 cggtcgagcg cccgcgccag cgccggcgcc ggggcaagcg gcgacaggtc gatgtcgtgg
     8461 acgaagtcga ggtagtggtc ggggtcgacg ccgtggtcga gcatcagccc gcggagcgtg
     8521 gtgccgtggc ggtggtagta gcccttctgg atcgcgcgcg cggccgcccg gtcgacgcca
     8581 agcaggctcg aaatatattg acccatgcgg tcgtcgatct gtgcgaacag gtcgcaatcc
     8641 gcggcataca acgtgttgtc gaggtcgaac acccagtcgt cgagcgtgtt cgcgcgggag
     8701 agatcgggct ccgggaccgg accaggcgca accatggccg caaggtgcaa caggggccgc
     8761 gagcgcgcaa gcgcccgctg gaggcgggcg tgcgcgccgg gcgggacggt gtcggccggc
     8821 cgacccggcc gcgccgggtc gtcccggttg tgagggattg aggacggtgc cggcaaccag
     8881 cacgctgatc ccgccctccg ggggtggaca cgcgggcgcg cctgagccgg tgcgctcgcc
     8941 ggcctcggag cagttccttg ccgcctatcc cgggccggcg ctgctcagcg acggcgccgg
     9001 gcgggtggtg atggccaacg cccaggccgg caacctgcag agcgcgatcg aggaaggcat
     9061 ggttcccgac ctcgccctgg cgctgtcgcg ggcgctggcg agcggggagt cccagaccgc
     9121 gaccgcgatc ctgccggcgc atctcggcgg cagcggcctc aacctgaccc tggtcgccgg
     9181 cggcgtcggc ctggtcggcg ccaagtgggt gctggtgctt ggcagcgacg cctcgctcga
     9241 gatcaacctg cgcaacgcgc tggtcgaatc gcgccaacgc tacaaggacc tggtcgactg
     9301 ctcgagcgac ttcgcctggg agaccggcaa ggacggccgg ttcgtgttcg tctcgcccgc
     9361 gggcgtgctc ggctaccacc ccgacgagct gatcgggcgc gcggcgcgcg acttcgtgtc
     9421 cgctgacttc gagccgcccg aggagctgcc gttcgacggc gcacgccgga tggaggacgt
     9481 ggtggtgtgg ttccgcgcgg ccggcggcgg caatgcctgc ctgaccgtct ccagcgtgcc
     9541 gctgttcggc cccgacggcg cctgggccgg ggcgcgcggg gtcgggcgcg acatcaccgc
     9601 gggccgggcc aaggacctgc tcttggcggt ggcgcggcgg cgcgagcagg tgctggacgc
     9661 ggtggcgcgc gcgacccgga tcgaggtcga ggcgccggcg atgctgatcg ccgcctcgcg
     9721 cgccgtcgcc gaggcgatgg agctgagcgc gtgctgggtg ctgcggctgg atgcgagccg
     9781 ccagccctat gtcgccgcca gctggccgcc ccgccagccg ccgccggcga gcgtgatgtc
     9841 gcgcctggcc gaggtcgtcg cccgcgccgc cgccgacgcg caccacgcgc tcgaggtgga
     9901 cggcgcgctg ctggtcccgg cacagcacgc ccagtgcgtc aacggcgcca tcgccgccct
     9961 ccagggaccg ggagattggg ggatcgagga ccgcgacctc atgtccgcgg tcgccggcca
    10021 ggtcggcatc ctgatcgagc aggcgtcgat ccacgagcag ctgcaggtca tgtcgcgcac
    10081 cgacccgctg accggcctgc tcaaccggcg cgcgttcctc gacgaggccg gccggcggct
    10141 cgcccacgcg acgcggaccg gccggggcgc cggcgtgctc tacatcgacc tcaacaactt
    10201 caaggcgatc aacgacgcct tcggccacgg cgcgggcgac cgcatcctgc gcaccgtcac
    10261 ccgcgtgatc gccgacaccg gccgcggcgg cgacgtcgcc tcgcgctacg gcggcgacga
    10321 gttcgtgatc tggctggacg agaccaatct cgccggcgcc aacgcgcggg cggaaaagct
    10381 gatcgcgctg atcgacaagc gcaccgacat gccggaggtg cccgggcgaa ggttcgggct
    10441 ggcgatcggc gtcgccgcgt tcgaccccgc gtccggcgag cagttggcgt cgctgctcga
    10501 gcgcgcggac cgggccatgt acgaggccaa gcgcgcccgt gacggctttg gcctcgagat
    10561 cgcgccggcg ttcgcaaggg aggccggaac agcgtgagcg acctggttcg tcaagaccac
    10621 cgcgcaccgc cggccggcgg cgaggcgagg tccgagtccc gcacggcgct ggcaagtggg
    10681 gccgagctca gctaccagca ggcgcgcgtg ctcgcccgcg acagggatgt cgccgtccgc
    10741 cgccggcttg ccgcgcgcga ggacgtggcg ccggagatcc tttactacct ggccgaggac
    10801 gccgatgccg aggtgcggcg tgcggtcgcg accaacccgg ccacccccag gcacgccgac
    10861 ctcaagctcg cgcgcgacgc gaacgacgac gtccgcatcg agcttgcgca caagatcgcg
    10921 cgcctggtgc cgggcctgcc cgcggccgag cgcgatgcgc tctaccggct cacgatcgag
    10981 gcgctgacca ccctcgcccg cgatgcgctg ccccacgtca ggcgcgtcat cgccgaggag
    11041 atcaaggcga tggacgcggt gccgcgcgag gtggtccagc gcctcgcgcg cgaccttgag
    11101 gtgatggtgg cggcgccggt gctcgagttc tcgccgctgc tgaacgacgc cgacctgctg
    11161 gagatcatcc acagcgcgcc ggtccgcggc gccttgtccg cgatctcgcg ccggcgtgcg
    11221 ctggccgcgc cggtatgcga tgcgatcgcg gccaccggcg aggtcgccgg cgtcgccgag
    11281 ctgctggcca acgccagcgc ccagatccgc gaggaaaccc tcgacaagct gatcgaccag
    11341 gcccacgcgc atcccgagtg gcagcggccg ctggcggtcc gcccgcaact gtccgagagc
    11401 gcggccaggc ggctgatgcg cttcgtctcg cgcgcgctgc tcaccgagct cgagcgcgcc
    11461 aataggctgg cgccggcggt ggcggcggcg ctggccgagc gggtcgaagc ccggctcgag
    11521 accggcggcg cggagtgcga agacgcggcc gcggccgagg accggtccga ggtcgccgag
    11581 cagatgcgcg ccgccgatcg gcgcggcgag ctcgaccagg ccgcgatcgg ccgcctgatc
    11641 agggacggca agctcgagca cctggtccag gcgctggcga tccgcgccga ggtcacctcg
    11701 ctcggcgtcc ggcgcatcat tcgctcagcc aacgccacgg cgctggtcgc gctcgcctgg
    11761 aaggccggcc tcgacatggc caccgccacc gggctccagg cgcgcatcgc caacatcgcg
    11821 cccgagctcc tgatccagcc cgcggccgac ggcggctatc cgatcgaggc cgacgagctg
    11881 gagtggcggc tctacctgtt caccgcctga tcgacgccaa cgaggctgcg acgcagtcgc
    11941 cgcaagggcc gcctcagcgt caggcccgcg caagagaccg cgactccttt gccgcaccct
    12001 tcgagacgcg cccttcgggc gctcctcagg gtgaggtgat tgcttgagtt tcaagtcttc
    12061 ctcgtcctga ggagcgagcg gagcgagcgt ctcgaaggac ggcacccgaa acattcacgc
    12121 gcgcttgcgc gtgggtgacg acacgggcgg gcggggccgg acagcctgtc acggcatgct
    12181 acgcggcgtg ggtcggcgcg cagcctgagc gcgcgatcat ggtgccgacc ccgtgcgcgg
    12241 tgaacacctc gagcagcagc acgtgcggga tgcgcccgtc gaggatgtgc gccgcctcgg
    12301 tgcccgcctc gatcgccgcg atgcaggtct cgatcttcgg caccatgccg cccgagacgg
    12361 tgccgtcgtc gatgcaggcg cgcgcggcct ccggcgtcaa ggtcgcgatc agcgcgccgt
    12421 cgcgccccag cacgccggcg acgtcggtca gcatcagcag cttggtcgcg ccgagcgcag
    12481 ccgcgatcgc gccggcggcg gtgtcggcgt tgatgttgta ggtctcgccg tcggcgccga
    12541 acccgatcgg cgcgatcacc ggcacgcagc ctgagcgcgc gagcatctcc agaacctcga
    12601 cgttgatcga ctcgggctcg ccgacgaagc cgagcggcac cacccgctcg aggttcgagt
    12661 cgggatcgcg catcttgtgc tcgagccggc gcgcgcggat caggtggccg tccttgccgc
    12721 acagcccgag cgcggtgccg ccggcgcggt tgatcgccga ggcgatctgc ttgttgatgc
    12781 tgccggcgag caccatctcg acaatctcga ccgtcgcccg gtcggtcacc ctgagcccgt
    12841 ggacgaacga cgagctgatc ttcagccgct cgagcatctt gccgatctgc ggcccgccgc
    12901 cgtgcacgac caccgggttg atgccgacct gcttgagcag caccacgtcg cgcgcgaacg
    12961 actcggcgag cgagtcctcg cccatggcgt ggccgccgaa cttgatcacg aaggtcttgc
    13021 cggcgaagcg gcgcatgtag ggcagcgcct ccgacaaggt cgccgcggtg tgcaggcgtt
    13081 cctccaggct gggcgcagcg gtctcggaca tggcgcgaat cttggcgagc ggttgcgggc
    13141 agccgcgttt ctaacccacc ggcgccggcg cggccagcgc cgcaagcgcg gcacgcaagg
    13201 cttcgatgcc ctggccgctg cgcgcgctgg tggcgatcac ctcggggtgg gcggccgggt
    13261 ggcgggcgag ggtggcggcg atggccgcga tccgctccgc ggcgaccgcc gcggtcagcc
    13321 ggtcggcctt ggtgagcacg atctggtagg cgaccccggc ggcgtcgagc atcgccgcga
    13381 ccggccggtc ggccgccatc agcccgtggc gggcatcgac caggaggcac acccggcgca
    13441 ggctcgcccg cccgcgcagg tagtcccgca ccagctcggt ccacgccgcc acctcgcgct
    13501 tgggcgcgcg cgcatagccg tagccgggaa gatcggccag catcagccgc cggccgaggt
    13561 cgaacaggtt gatctggcgc gtgcgcccgg gagtggcgga gatgcgcgcc agcgcgcgct
    13621 ggccgaccag ggcgttgatc aggctcgact tgccgacgtt cgagcggccg gcgaaggcgg
    13681 cctcgggcag ctccgccgcc ggcagctggt cgaggcgcac cgccccggcg acgaaccggc
    13741 agggttgtgc gaacagccgg cgtgcggcct cgatcgcagc ggcctcgggc tcgcatgcag
    13801 cggccaccgg ctcaggcccg cacccccatc cgccgcatga tcacccattg ctgggcgatc
    13861 gacagcaggt tgttccacgt ccagtagatc accagcccgg ccgggaagcg cgcgaacagg
    13921 aaggtgaaca tcaccggcag cagcatcatg atcttcgcct gggtcggatc gggcggggtc
    13981 gggttgagct tctgctggag gaacatggtc acccccatca acagcggcca cacgccgatc
    14041 gacaggaagt ccggcagggt cagcggcagc agcccgaaca cggtgaacag gttggtcggg
    14101 tcgggcaccg acaggtcgtg gatccagccg tagaacggcg catgccgcat ctcgatggtc
    14161 acgaacagca ccttgtagag cgcgaagaac accgggatct ggaccacgat cggcaggcag
    14221 ccggccagcg ggttggcctt ctcgcgcttg tacagcgcca tcacctcggc gttgagcttc
    14281 tgcttgtcgt cgccgcactg ctcgcgcagc ttcatcatct ccggctgcag cttcttcagc
    14341 ttgctcatcg cggtatagga cttgttcgcc agcgggtaga acacgagctt gatcagcacc
    14401 gtcagcagca ggatcgccag gccgaaattg ccgagccagc ggctgaacgt gtggatgacg
    14461 tagaacagcg g
//
LOCUS       DASWHU010000210        15133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1593324, whole genome shotgun sequence.
ACCESSION   DASWHU010000210 DASWHU010000000
VERSION     DASWHU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593324"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1954)
                     /locus_tag="VGB88_07715"
     CDS             complement(254..1954)
                     /locus_tag="VGB88_07715"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX9647369.1"
                     /translation="MSRVADQHARPLSDLEIARAARLSPILEVAADKLGLDADTLEPY
                     GRDKAKLSLDAIAGLEGRADGKLILVTAITPTPAGEGKTTTTVGLGDALNAIGARAIV
                     CLREPSLGPCFGMKGGAAGGGYAQVVPMEDINLHFTGDFHAIGIAHNLLAALIDNHIY
                     WGLEPAIDSRRVAFRRVVDMNDRALRQITSSLGGVANGFPRTDGFDIVVASEIMAIFC
                     LAKDLADLTNRIGNIVIGHNRQREPIYARDVKGPGPMSVLLKEALAPNLVQTLENNPA
                     FIHGGPFANIAHGCNSVIATRTALKLADYVVTEAGFGADLGAEKFFDIKCRKAGLTPS
                     AAVIVATVRALKMHGGVAKDGLGRENVEAVKKGCENLARHITNVQKFGVPVVVAINQF
                     ITDTDAELAAVRETAKGFGANAIVASHWANGAAGIEELAYHVVELVDSGKADFKPLYP
                     DEMPLIDKVRTIAQTIYGADDIACDGAILKQFDELQKDYGHFPVCMAKTQYSFSTDPN
                     LKGAPSGFTIPIRELRLSAGAEFVVAITGEIMTMPGLPKVPSANSIFLDDEGRVQGLF
                     "
     gene            2047..2406
                     /locus_tag="VGB88_07720"
     CDS             2047..2406
                     /locus_tag="VGB88_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9647370.1"
                     /translation="MAVINIRAGGALVAACVLAAVAGAAPAGATSAAGRTLAERDCAP
                     CHAVGRTGESPVAAAPPFRTFAARWPVEHLREALAEGVMVGHGGIEMPEVALAPEQID
                     HLLAHLAALAGAGDDAR"
     gene            2393..3076
                     /locus_tag="VGB88_07725"
     CDS             2393..3076
                     /locus_tag="VGB88_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647371.1"
                     /translation="MTRASSRLGALAVVLAAAAAPAPAVAAEVAWTDDLPYGLAWGMT
                     LKQVLDAGIRVEYGKLQGEFGHEYLAFDLPPQLGDEDQVFLYFGYDDRLWRIAVHSRG
                     YSDEGTGTALVKRYDELKAALDARYGAGEEFLHLHDSAVAVPKRTVGTIQIGASWRFT
                     EYRTPGEHIQLGIRARNLWTGYIALYIKNLDMEPKAIADAEANAKTVAAGEETPFPAP
                     HLSLEQDTD"
     gene            complement(3082..4722)
                     /locus_tag="VGB88_07730"
     CDS             complement(3082..4722)
                     /locus_tag="VGB88_07730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX9647372.1"
                     /translation="MHLDTVKALALSAVLVIAGADSGRAGAIHPDGTDRDCTRLNLAA
                     QNTVVTRWNLAALEAICAGRPLPTVVARELFLLHAAIFDAWAAYDETARPLYWEGFVR
                     RPAAERSAAAKSEAISFAALEVLLELYPDHAVEFLDRLHREGFDDRDFLAPPPGSPAR
                     IGIAAAGAVLDRRAGDGANSAGGYADISGYRPANAPDAGLGDIDPNRWQPLVVTDFDP
                     ARRPTGFVLDPQRFDPEAYGRFAPQMFVTPHWAWIEPFALDGGDQLRPPPPPQYGNHE
                     PYIDARGRQTTYHQAYIDQFDEVMEMQAALTDEQMIIAQVWSHDGPYFAAPSGHWNHF
                     AQQISLRDRHGTDDDVKLFLALNGALLDASIAAWDAKIAYDSVRPITAIRFLHRGHRI
                     TGWQGRDHGIGPMRGEDWIPYQWTAWASPAFPEYVSGHSTFAAAAAEVLRLFTGSDRF
                     YDPAVRVGDLDRDRIREPVGSYVVRHRYGAFDRAIPVNDVTLRWDTFTAAAEQCGLSR
                     LYAGVHITDANLRALAMGRIAGAQAYRRAERLWQGEGG"
     gene            5028..7967
                     /locus_tag="VGB88_07735"
     CDS             5028..7967
                     /locus_tag="VGB88_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9647373.1"
                     /translation="MSSKRVAGRVALSFVLISTVLSGPPHANDKLVYAATETTYSLSG
                     GAKVVRVGHPGGEVLPTADSNESFAAAGVERRTVRVARGQTLADILLAAGVDRIEAAR
                     ASKALAGQVNLRGLQIGQRLELFLAAQRRPSIAKTLIGLALDLRGGQQAVVYRDFGRA
                     YAVRRMSPQAATALLQSIVVVDAKGEPGFVARDLVLRKGQNINRLLIESGASATDGHG
                     AANKLNQQVDLHRLQIGQRVTATFETFTDGRAPRLAGVAMTLPGGRVAVVGRDAHDHW
                     RAGPAALAGATPAAPAAPAAEKAPELPVVVALGERPPPAHTESVTIVLDRGDILYNRL
                     IKAGATSAEADRAVADLAKVLNLRKLQIGEQFRLGFTAPAEGGRAQLTGLSVTVGKED
                     VVVLGWPVVGSGRAQVAAAPSASIKHVAPAEEAASAGEGGEAETAPPPTDSTASADVR
                     LLTVARGDTLVSLLTKAGIGRSESGQVLQALRRVYNPNRLSIGQRLVVEIDRDGEAAG
                     LATLTLEAGTRSAVTVERARSGRYAARKEGREALEAALAFAGARGRARASVKVAAGAA
                     ADADADPESLAARVNGDQAVDLERGARKIVTLDRGGTLIEALVEAGSTAPEAHAAIES
                     VRELYNPRRLQAGQAVSLTFVAARSDASLFATAVPTLAGEEPPVLGGLTLDVSTEKRL
                     EVVRLEDGRFVSGFVAKDLVSDYRRAEGTIRSSLYEAIIDAGVPVAVMMELIRIYSFD
                     VDFQRDIHDGDRFELMYEMLVDEAGEAVKPGAILYAALELSGAVKEMYRYRLADGRIE
                     YFDAQGRSVRKALLRTPVSGAELSSGFGMRKHPILGYSKMHKGVDFAARRGAPVFAAG
                     EGVVERASRFGAYGNYVRIRHSPEYATAYAHLNGFAKGIYPGKRVVQGQTVGYVGTTG
                     RSTGPHLHYEVLLRDKQVNPLSVKLPTGIKLADNERATFLAERERLRTLYAELPSGRE
                     VAQGGSP"
     gene            complement(8045..10642)
                     /gene="clpB"
                     /locus_tag="VGB88_07740"
     CDS             complement(8045..10642)
                     /gene="clpB"
                     /locus_tag="VGB88_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX9647374.1"
                     /translation="MNVDRYTERSRGFIQSAQARAVAAGHQRFTPEHLLKELLDDRDG
                     LAANLMRAAGGRPDQALQLVEAELARLPKVEGGGAGQLYLAPETARLFESAEKIAEKA
                     GDKFVTAERLLLALALAKGTAAARALADSGLTPDGLNAAINELRKGRTADSASSEDSY
                     DALKRYARDITELARAGKLDPVIGRDEEIRRCIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLANRMVSGDVPESLRDKRLLALDLGSLVAGAKYRGEFEERLKAVLAEIQAAEGEIV
                     LFIDELHTLVGAGAAEGAMDASNLLKPALARGELHCIGATTLDEYRKRIEKDAALARR
                     FQSVFVSEPSVEDTISILRGLKEKYELHHGVAITDGAIVAAATMSARYITDRYLPDKA
                     IDLIDEAASRKRMEMDSKPEQLDELDRRVIQLKIEREALRKETDAASRERLKKLESEL
                     ADIESRSASLTAEWQAAKEHLHGAQDIKERLEAARNELERATREGNYERAGELNYGII
                     PDLERRLAASQGPDGGTRLTVSEEVTADDIASVVSRATGIPVDKMMEGERDKLLHMEE
                     AIHRRVVGQDDAIQAVANAVRRARAGLQDPNRPIGSFLFLGPTGVGKTELTKALAEFL
                     FDDEAAFVRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEVE
                     KAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTLIVLTSNLGSDVLASLPDGADVSSAR
                     EPVMALVRAAFRPEFLNRLDEIVLFHRLSREQMGGIVDIQLDRLRHLLAGRDIRIELD
                     QAAKTWLANAGYDPVYGARPLKRAIQRNLQDPLATMILGGRIQDGDVVDAGVAEGALA
                     LSPRPRAAA"
     gene            complement(10789..11244)
                     /locus_tag="VGB88_07745"
     CDS             complement(10789..11244)
                     /locus_tag="VGB88_07745"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX9647375.1"
                     /translation="MKQSLNSKRHHRGRGSGRRQGHGFASSFESSGPDVKIKGSASHV
                     FERYQSLARDAIAAGDRVAAENFLQHAEHYQRIMNAHLAAAGQQPDANGQGRQINGRD
                     DDDERAQDGGTGPFGDEGPVRVRGRARSRPGVEPDSDDDETGSSDPFRL"
     gene            complement(11415..12332)
                     /gene="prmC"
                     /locus_tag="VGB88_07750"
     CDS             complement(11415..12332)
                     /gene="prmC"
                     /locus_tag="VGB88_07750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9647376.1"
                     /translation="MSARPGPPATVAEAIARAAARLAALPATDARREARLLLAHASGL
                     APGQLIADPDRRLLPDAAADFQALVGRRLAREPIAYILGEREFWSLSFAVTPAVLVPR
                     PDSETVVEAALAAFAAGAGAGTVLDLGTGSGCLLLAVLSEWPGARGLGIDSDPGAVAL
                     ARANARRLGLDARARFAVGDWGRAVGARFDLVVANPPYVPSAAIAHLAAEVARHEPRR
                     ALDGGGDGLDAYRALVPDLGRLLVPGGVAVLEVGAGQAAAVARLLGLAGLVAVARHLD
                     LAGIARCLSARAPAPVGNQKTLGHCVVGR"
     gene            complement(12329..13402)
                     /gene="prfA"
                     /locus_tag="VGB88_07755"
     CDS             complement(12329..13402)
                     /gene="prfA"
                     /locus_tag="VGB88_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9647377.1"
                     /translation="MDLDAKLDRIVERHHELGELMAGGAPSSEQRVSLAREYAELAPV
                     VAKIEALRKLKREIRDLAELARDAAGGEEMRALAAAELDGCRAREGELDHALRLALLP
                     RDEADEKSAILEIRAGTGGEEAALFAATLFRMYQRYAEIKGWRFETLHLSETGLGGFK
                     EAVAAIKGRSVFARLKYESGVHRVQRVPRTEAQGRVHTSAATVAVLPEAEEVDVRIDD
                     KDLRIDVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSQQDEKSQHKNRAKAMRILRAR
                     VYERERSRREAERAASRRAQVGSGDRSERIRTYNFPQGRVTDHRIAMSYYDIDAVIDG
                     AALDEFIDALIADDQASQLAALA"
     gene            complement(13454..14593)
                     /gene="ispG"
                     /locus_tag="VGB88_07760"
                     /gene_synonym="gcpE"
     CDS             complement(13454..14593)
                     /gene="ispG"
                     /locus_tag="VGB88_07760"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9647378.1"
                     /translation="MSVRPYRDIARRKSRQIRVGAVAVGGEAPISVQSMTNTPTTDVA
                     ATVAQVQALEAAGADIVRISCPDAESSAALKRIVPQVGVPIVADIHFHYKRAIEAAQA
                     GAACLRINPGNIGAAERVREVVKAAKDHGCSMRIGVNAGSLERDLLEKYGEPCPEAMV
                     ESALDHARILEDNDFFEFKISVKASDVFLAVAAYQGLAEACDYPLHVGITEAGGLRGG
                     TVKSSIGLGMLLWSGIGDTVRVSLSADPVEEVKVGFELLKALSLRHRGVSIIACPSCA
                     RQQFQVIRTVELLEQRLAHISTPLSLSVIGCVVNGPGEARETDIGLTGGGKGTHQVYL
                     SGVTDHKIDDARLVDHLVELVERKAAEIEAAEADEDEPAAEPVAS"
     gene            complement(14694..>15133)
                     /locus_tag="VGB88_07765"
     CDS             complement(14694..>15133)
                     /locus_tag="VGB88_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEX9647379.1"
                     /translation="ASPFGADSRPGSATVITGALAATPDAPDLIEDPPPPPAAPAAGY
                     EPRVYGQGNADARIVIRARADAWVQVQGANNDLILTRILRPGDVFLVPDRDDLTLVTG
                     NAGGLEVLVDGVPAPPLGTEGSVRRAIRLTPEALASGGTVGQR"
BASE COUNT         2110 a         5320 c         5450 g         2253 t
ORIGIN      
        1 tcattcccgc cccaaactcg tcattcccgc gcaggcggga acccaggagc ggcacacggc
       61 gcggctgcgg tccgtcgcgc tgggtccctg ccttcgcagc gatgacggtt agggggcagg
      121 gatgacggat ggaggcaggg atgacggatg gaaaaaaagc ccccgggatc gtctcccggg
      181 ggcctcgtcg cagctgttgt ccgggcgcga gcgggaggga gtcgaacgcc cgctgcccgg
      241 attcgccgct ggatcagaac aggccctgca cccggccctc gtcgtcgagg aagatgctgt
      301 tggccgaggg caccttcggc aggcccggca tggtcatgat ctcgccggtg atcgccacca
      361 cgaactccgc ccccgccgac agcctgagct cgcggatcgg gatggtgaag cccgagggcg
      421 cccccttgag gttcggatcg gtcgagaagc tgtactgagt cttggccatg cacaccggga
      481 agtggccgta gtccttctgc agctcgtcga actgcttcag gatggcgccg tcgcaggcga
      541 tgtcgtcggc gccgtagatc gtctgggcga tggtgcgcac cttgtcgatc agcggcatct
      601 cgtcggggta gagcggcttg aaatccgcct tgccgctgtc gaccagctcg accacgtggt
      661 acgccagttc ctcgatgccg gcggcgccgt tggcccagtg gctggcgacg atcgcgttgg
      721 cgccgaagcc cttggcggtc tcgcgcaccg ccgcgagctc cgcatcggtg tcggtgatga
      781 actggttgat cgccaccacc accggcacgc cgaacttctg gacgttggtg atgtggcggg
      841 cgaggttctc gcagcccttc ttcaccgcct cgacattctc gcgtccgagc ccgtccttgg
      901 cgacgccgcc atgcatcttg agcgcccgca ccgtggccac gatcaccgcc gccgaggggg
      961 tgagcccggc cttgcggcac ttgatgtcga agaacttctc ggcaccgaga tcggcgccga
     1021 agccggcctc cgtaaccacg tagtcggcga gcttgagggc cgtccgcgtg gcgatcaccg
     1081 agttgcagcc atgggcgatg ttggcgaacg ggccgccatg gatgaaggcc gggttgttct
     1141 ccagcgtctg caccaggttg ggcgccagcg cctccttcag cagcaccgac atcgggccgg
     1201 ggcccttgac gtcgcgggca tagatcggct cgcgctgacg gttgtggccg atgacgatgt
     1261 tgccgatccg gttcgtgagg tcggcgaggt ccttggccag gcagaagatc gccatgatct
     1321 ccgaggccac cacgatgtcg aagccgtcgg tgcgcgggaa gccgttggcg accccgccga
     1381 gcgaactggt gatctgcctg agcgcccggt cgttcatgtc gaccacccgg cggaaggcca
     1441 cgcggcgcga atcgatcgcc ggctccagcc cccagtagat gtggttgtcg atcagcgccg
     1501 ccagcaggtt gtgggcgata ccgatggcat ggaagtcacc ggtgaaatgg aggttgatgt
     1561 cctccatcgg caccacctgg gcatagccgc cgccggcggc gccgcccttc atgccgaagc
     1621 acgggccgag gctcggctcg cgcaggcaga cgatggcgcg cgcgccgatc gcattcagcg
     1681 catcgcccaa gccgaccgtg gtcgtggtct tgccctcgcc cgccggcgtc ggcgtgatcg
     1741 cggtgaccag gatcagcttg ccgtcggcgc gcccctcgag cccggcgatg gcgtcgagcg
     1801 acagcttggc cttgtcgcgg ccatacggct cgagcgtgtc ggcatcgagg ccgagcttgt
     1861 ccgccgcaac ctcaaggatc ggactcaggc gcgccgcgcg cgcgatctcg agatcgctga
     1921 gcggtcttgc gtgctggtcg gcgactctgg acattggcgc gggcctcttc tggaatatgt
     1981 tcgcaacagg cggccggccc agccggctgg gccggaacgg ctgccggcag gagagtgatc
     2041 ggcagggtgg cggtaatcaa catcagggcc gggggcgcac tcgtcgccgc ctgcgtgctc
     2101 gccgcggtgg cgggcgctgc gccggcgggc gcgacctccg cggccggccg gacgctggcc
     2161 gagcgcgact gcgccccctg tcacgcggtc gggcggaccg gggagagccc ggtggcggcg
     2221 gcgcccccat tccgcacctt cgccgcgcgc tggccggtcg agcatctgcg cgaggcgctg
     2281 gccgaagggg tgatggtcgg gcatggcggc atcgagatgc cggaggtcgc gctcgccccc
     2341 gagcagatcg accacctgct cgcccatctg gccgccttgg ccggcgcggg cgatgacgcg
     2401 cgctagctcc cgcctcggcg cgctcgcggt cgtgctggcc gccgccgcgg cgccggcgcc
     2461 ggccgtcgcg gccgaggtcg cgtggaccga cgacctgccc tacgggctcg catgggggat
     2521 gacgctcaag caggtgctcg atgccggcat ccgggtcgag tacggcaagc tccagggcga
     2581 gttcggccac gagtacctgg ccttcgacct gccgccgcag ctcggcgacg aggaccaggt
     2641 gttcctctac ttcggctacg acgaccggct gtggcgtatc gccgtccaca gccggggcta
     2701 ctcggacgaa gggaccggaa ccgccttggt caagcgctac gacgagctca aggccgcgct
     2761 cgatgcgcgt tacggggcgg gcgaggagtt cctgcacctg cacgactcgg cggtggcggt
     2821 gcccaagcgg acggtgggca ccatccagat cggggcgtcg tggcggttca cggagtaccg
     2881 gacgccgggc gagcacatcc agctcggcat ccgcgcgcgc aacctgtgga ccggctacat
     2941 cgcgctctac atcaagaacc tcgacatgga gcccaaggcg atcgccgacg ccgaggccaa
     3001 cgccaaaacg gtggccgccg gcgaggaaac gccgttcccc gcgccgcacc tgtcgctcga
     3061 gcaagacacg gactaggccg ctcagccgcc ctcgccctgc cacaggcgct cggcgcgccg
     3121 gtaagcctgg gcgccggcga tgcgccccat cgccagtgcg cgcaggttcg cgtccgtgat
     3181 gtgcaccccg gcatagagcc gcgacaggcc gcactgctcg gcggcggcgg tgaaggtgtc
     3241 ccagcggaga gtcacgtcgt tgaccgggat cgcgcggtcg aaggcaccgt agcgatggcg
     3301 cacgacgtag ctgccgaccg gctcccggat ccggtcgcgg tcgaggtcgc cgacgcgcac
     3361 cgcgggatcg tagaagcggt cgctgccggt aaacagcctg agcacctcgg ccgccgccgc
     3421 ggcgaaggtc gaatggccgg agacgtattc gggaaaggcg ggcgaggccc aggcggtcca
     3481 ctgatacgga atccagtcct cgccgcgcat cgggccgatg ccgtggtcgc gaccctgcca
     3541 gccggtgatc ctgtggcccc ggtgaaggaa gcggatggcg gtgatcgggc gcacggaatc
     3601 atatgcaatc ttcgcgtccc aggcagcgat cgaggcgtcg agcagcgcgc cgttcagcgc
     3661 caggaacagc ttcacgtcgt cgtccgtgcc gtgccggtcg cgcagcgata tttgctgcgc
     3721 gaagtgattc cagtgcccgg acggcgcggc gaagtacggg ccgtcgtggg accacacctg
     3781 ggcgatgatc atctgctcgt cggtcagcgc ggcctgcatc tccatgacct cgtcgaactg
     3841 gtcgatatag gcctggtgat aggtcgtctg ccggccgcgg gcgtcgatgt agggctcatg
     3901 attaccgtac tggggcggcg gcggcgggcg cagctggtcg ccgccgtcga gcgcgaacgg
     3961 ctcgatccac gcccagtgcg gggtgacgaa catctgcggc gcgaagcggc cataagcctc
     4021 ggggtcgaag cgctgcggat cgagcacgaa gccggtcggc ctgcgggccg ggtcgaagtc
     4081 ggtcacgacc agcggctgcc agcggttggg gtcgatgtcg ccgagcccgg catcgggcgc
     4141 gttggccggc cgatagccgc tgatgtcggc atagccgccg gcgctgttgg cgccgtcgcc
     4201 ggcgcgccgg tcgaggaccg cgccggcggc cgcgatgccg atgcgggcgg ggctgccggg
     4261 cggcggggcg aggaagtcgc ggtcgtcgaa gccctcgcgg tgcagccggt cgaggaactc
     4321 gaccgcgtgg tcgggataga gctcgagcag cacctcgagc gcggcgaagc tgatcgcctc
     4381 ggacttcgcc gccgcactgc gctcggccgc cggccggcgc acgaagccct cccagtagag
     4441 cgggcgcgcg gtctcgtcat aggcggccca ggcgtcaaaa atcgcggcat ggagaaggaa
     4501 taattctcgc gccaccacgg tgggcaacgg tcgcccggca cagatcgcct cgagcgcggc
     4561 gaggttccag cgcgtgacca ccgtgttctg cgccgccagg ttgagccgcg tgcagtcgcg
     4621 gtccgtgccg tccggatgga tcgcgccggc gcgcccggaa tcggcgccgg caatcacgag
     4681 cacggcagac agtgccaggg ccttgactgt gtccaagtgc atggccgctc ccgcccggac
     4741 caagccaggc tcacaacttg gcgcccgcga tcgccgctgt cgagcccgtt tgggcgccga
     4801 cggcaggcgc gctgactggt cgggtccgcg ccggggcgcc atataggtag tgttacgcgc
     4861 gtttgcgtcg gccctgggtc gatacggcgg acgcgttaac cattgttgac aaacaccacg
     4921 atggcgaaca tggttgccgt cgcttctggc tcggccccgg gggaaagcgg ggaagacgtc
     4981 agggggagtg acttcgaggc gggtcggaat ccgagtggga gcgggggatg agttcgaagc
     5041 gcgtggcggg tcgcgtcgct ctttcgtttg ttttgatcag tacagtcctt tctggcccac
     5101 cccacgccaa cgacaagctc gtctacgcgg caactgaaac cacctatagc ctgtccggcg
     5161 gcgccaaggt ggtgcgcgtc ggccatccgg gcggcgaggt cctgcccact gcagactcga
     5221 atgagagctt cgccgccgcc ggcgtcgagc gacgcacggt gcgcgtggcg cgtggccaga
     5281 cgcttgccga cattcttctt gccgccggcg tcgaccggat cgaggcggcg cgcgcgagca
     5341 aggcccttgc cggccaggtc aacctgcgcg gcctgcagat cggccagcgg ctggagctgt
     5401 tcctggcggc gcagcggcgg ccctcgatcg ccaagaccct gatcgggctc gcgctcgacc
     5461 tgcggggcgg ccagcaggcg gtggtgtatc gcgatttcgg ccgcgcctac gcggtccgcc
     5521 gaatgagccc gcaggcggcg accgcgctgc tgcagtcgat cgtggtcgtc gacgccaagg
     5581 gcgagcccgg attcgtggcc cgcgacctgg tgctcaggaa aggccagaac atcaatcgcc
     5641 tgttgatcga aagcggcgcc agcgcaaccg acggccatgg tgcggccaac aagctgaacc
     5701 agcaggtcga cctgcaccgg cttcagatcg gccagcgggt gaccgccacc ttcgagacct
     5761 tcaccgacgg ccgcgcgccg cgcctcgccg gcgtcgcgat gaccctcccc ggcggccgcg
     5821 tcgccgtggt cggtcgcgac gcgcacgacc attggcgcgc cgggccggct gcgcttgccg
     5881 gcgccacgcc ggcggcgccg gccgcgccgg ctgcggaaaa ggcgccggag ttgccggtgg
     5941 tggtggcgct cggcgagcgg ccgcccccgg cccataccga gtccgtcacc atcgtgctcg
     6001 accgcggcga catcctgtac aaccgtctga tcaaggccgg cgcgaccagc gccgaggccg
     6061 atcgggccgt cgccgacctg gccaaggtcc tcaacctacg caagctccag atcggcgagc
     6121 agttccgcct cggcttcaca gcgccggccg agggcggccg ggcgcagctg accgggctgt
     6181 cggtgacggt cggcaaggag gacgtggtag tgctcggctg gccggtggtc ggcagcggcc
     6241 gggcgcaggt cgcggccgcg ccctcggcct ctatcaagca tgtcgcaccg gcagaggagg
     6301 ccgcctccgc cggcgagggc ggcgaggccg agaccgcccc gccgccgacc gactccacgg
     6361 cctcagccga cgtgcgcctg ttgacggtcg cgcgcggcga tacgttggtg agcctgctga
     6421 ccaaggccgg catcgggcgc agcgagagcg ggcaggtcct gcaagccctg cgccgcgtct
     6481 acaaccccaa ccggctgagc atcggccagc gcctggtggt cgagatcgac cgcgacggcg
     6541 aggcggccgg gctcgccacg ctgacgctcg aggccggcac ccgcagcgcg gtgaccgtgg
     6601 aacgcgcccg gtccgggcgc tatgcggcgc gcaaggaggg ccgcgaggcg ctcgaggccg
     6661 cgctcgcctt tgccggcgcc aggggccgag ctcgggcctc ggtcaaggtc gcggccggcg
     6721 ctgccgcgga cgccgatgcc gatcccgagt cgctcgccgc ccgggtcaat ggcgatcagg
     6781 ccgtcgacct ggagcggggt gcgcgcaaga tcgtgacgct ggacaggggc ggcacgctga
     6841 tcgaggcgct ggtcgaggcc ggctcgaccg ccccggaggc ccacgccgcc atcgagtcgg
     6901 tgcgcgagct gtacaacccg cgccggctgc aggccggcca ggcggtgtcg ctgaccttcg
     6961 tcgcggcgcg ctcggacgcg tcgctgttcg cgaccgcggt accgactctg gccggcgagg
     7021 agccgccggt tctgggcggc ctcaccctcg acgtcagcac cgaaaagcgg ctcgaggtgg
     7081 tgcgcctgga ggacggccgg ttcgtctcgg gcttcgtcgc caaggacctg gtcagcgact
     7141 accggcgcgc cgaagggacc atccgttcca gcctctacga ggcgatcatc gatgccgggg
     7201 tcccggtcgc ggtgatgatg gagctgatcc gcatctacag cttcgacgtc gacttccagc
     7261 gcgacattca cgacggcgac cggttcgagc tgatgtacga gatgctggtc gacgaggccg
     7321 gcgaggcggt caagccgggc gcgatcctgt acgcggcgct cgagctgtcg ggcgcggtca
     7381 aggagatgta ccgctatcga ttggcggacg ggcggatcga gtatttcgat gcccagggcc
     7441 ggagcgtccg caaggcgctg ctgcgcacgc cggtcagcgg cgccgagctg tcgtcggggt
     7501 tcggcatgcg caagcacccg atcctcggct actcgaagat gcacaagggc gtcgacttcg
     7561 cggcgcggcg cggtgcgccg gttttcgccg cgggcgaggg cgtggtcgaa cgcgcctcgc
     7621 gcttcggcgc ctatggcaat tacgtgcgca tcaggcacag tcccgaatac gccaccgcct
     7681 acgcccacct caacggcttc gccaagggca tctatcccgg caagcgcgtg gtccagggcc
     7741 agacggtcgg ttatgtcggc accaccgggc ggtcgaccgg gccgcatctg cactacgagg
     7801 tgctgctgcg cgacaagcag gtcaacccgt tgagcgtcaa gctgccgacc gggatcaagc
     7861 tcgccgacaa cgagcgcgcg acgttcctgg ccgaacgcga gcggctgcgc acgctgtacg
     7921 ccgagctgcc cagcggccgc gaggtggccc aaggcggcag cccctgagcc ccgtcgcggc
     7981 gcgggtctct ctcgattgac acgggcggcc gatcggccgc ccgcggcttt tcggccgacg
     8041 cggctcaggc ggcggcgcgg gggcgcgggc tgagcgcgag cgcgccctcg gcgacgcccg
     8101 cgtcgaccac gtcgccgtcc tggatgcgcc cgcccagaat catggtcgcg agcgggtcct
     8161 gcaggttgcg ctggatcgcg cgcttgagcg gccgggcgcc atagaccggg tcgtagccgg
     8221 cattggccag ccaggtcttg gccgcctggt cgagctcgat ccggatgtcg cgcccggcca
     8281 gcaggtggcg cagacgatcg agctggatgt cgacgatgcc gcccatctgc tcgcggctga
     8341 gccggtggaa cagcacgatc tcgtcgaggc ggttgaggaa ctcgggccgg aacgcggccc
     8401 gcaccagcgc catcaccggc tcgcgcgcgc tgctcacgtc ggcgccgtcg ggcaggctcg
     8461 ccagcacgtc gctgcccagg ttcgaggtca gcacgatcag cgtgttcttg aagtcgacgg
     8521 tgcggccctg gccgtcggtc aggcggccgt cgtcgagcac ctgcaagaga acgttgaaca
     8581 cgtccgggtg cgccttctcg acctcgtcga acagaaccac ctgatagggc cgccggcgca
     8641 ccgcctcggt cagcgcgccg ccctcctcgt agccgacata gccgggcggg gcgccgatca
     8701 ggcgcgagac cgcgaacttc tccatgtact ccgacatgtc gatgcgcacg aacgccgcct
     8761 cgtcgtcgaa caggaactcg gccagcgcct tggtcaattc ggtcttgccg accccggtgg
     8821 ggcccaggaa caggaacgag ccgatcggcc ggttcgggtc ctgaagcccg gcgcgcgcgc
     8881 ggcgcacggc attcgccacc gcttgaatcg cgtcatcctg gccgaccacg cggcgatgga
     8941 ttgcctcttc catgtgcaag agcttgtcgc gctcgccctc catcatcttg tcgaccggga
     9001 tgccggtggc gcgcgagacg acgctcgcaa tgtcatcagc ggtcacctcc tcgctcaccg
     9061 tcaggcgcgt gcccccatcc gggccctggc tcgcggcaag gcggcgctcg agatcgggga
     9121 tgatgccgta gttgagctcg ccggcgcgct cgtaattgcc ctcgcgcgtc gcccgctcga
     9181 gctcgttgcg cgccgcttcc aggcgctcct tgatgtcctg ggcgccgtgc aggtgctcct
     9241 tcgccgcctg ccactcggcg gtcagcgacg ccgagcgcga ttcgatgtcg gcaagctccg
     9301 actcgagctt cttcagccgc tcgcgcgagg ccgcgtccgt ctccttgcgc agcgcctcgc
     9361 gctcgatctt gagctggatc acgcgccggt cgagctcgtc gagctgctcg ggcttgctgt
     9421 ccatctccat gcgcttgcgg ctcgcggcct cgtcgatcag gtcgatcgcc ttgtcgggga
     9481 ggtaccggtc ggtgatgtag cgcgccgaca tggtcgcggc ggcgacgatg gcgccgtcgg
     9541 tgatcgccac cccgtgatgg agctcgtact tctccttcag cccgcgcagg atcgagatcg
     9601 tgtcctcgac gctcggctcg gacacgaaca ccgactggaa gcggcgcgcg agcgccgcgt
     9661 ccttctcgat gcgcttgcgg tactcgtcga gcgtggtcgc gccgatgcag tgcagctcgc
     9721 cgcgcgcgag cgccggcttg agcaggttcg aggcgtccat cgcgccctcg gcggcgccgg
     9781 cgccgaccag ggtgtgcagc tcgtcgatga acagcacgat ctcgccctcg gcggcctgga
     9841 tctctgccag caccgccttc agccgctcct cgaactcgcc gcgatacttg gcgccggcga
     9901 ccagcgagcc cagatcgagc gccagcaatc gtttgtcgcg caacgattcg ggcacgtcgc
     9961 ccgagaccat gcggttggcc agcccctcga cgatcgcggt cttgccgacg ccgggctcgc
    10021 cgatcagcac cgggttgttc ttggtccggc gcgacagcac ctggatgcag cggcggatct
    10081 cctcgtcgcg cccgatcacc ggatcgagct tgccggcccg cgcaagctcg gtgatgtcgc
    10141 gcgcatagcg cttgagcgca tcgtaggagt cctcgctcga cgccgagtcg gcggtgcgcc
    10201 ccttgcgcag ctcgttgatc gccgcgttca ggccgtccgg cgtgagtccc gaatcggcga
    10261 gcgcccgggc cgcggcggtg cccttggcaa gcgcgagcgc gagcaggagg cgctcggcgg
    10321 tgacgaactt gtcgccggcc ttctcggcga tcttctcggc ggactcgaac aggcgcgcgg
    10381 tctcaggcgc gagatagagc tggcccgcgc cgccgccttc gaccttgggc agcctggcga
    10441 gctcggcctc gaccagctgc agcgcctggt ccgggcgccc gcccgccgcg cgcatcaggt
    10501 tcgccgccag cccgtcgcgg tcgtcgagca gctccttgag caggtgctcg ggcgtgaatc
    10561 gctggtggcc ggcggcaacg gcgcgcgcct gcgccgactg gatgaagccc cggctgcgct
    10621 cggtgtagcg gtcgacgttc atgcctgtcc cctcatgccg ctatggcatc cggcgacgcc
    10681 ggttgccgac gggacgcgcc aaagcggtcg ccggtctcgg cgacatataa tgtggcagta
    10741 ttgcaacaca aggcccggtg cgcgagggcg cgccggcgtc ctcggcggtt acaggcggaa
    10801 cggatccgag gagccggtct cgtcgtcgtc ggagtccggc tctaccccgg gccggctgcg
    10861 ggcgcggccg cgcacgcgca ccggcccctc gtcaccgaac ggccccgtcc cgccgtcctg
    10921 agcgcgctcg tcgtcgtcgt cgcgaccgtt gatctggcgg ccctggccgt tggcgtcggg
    10981 ctgctggccc gccgcggcga ggtgggcgtt catgatgcgc tgatagtgct cggcgtgctg
    11041 gaggaagttc tctgcggcga cgcggtcgcc ggcggcgatc gcgtccctgg cgagcgactg
    11101 gtaacgctcg aagacgtggc tggcgctgcc cttgatcttc acgtccggcc cgctgctctc
    11161 gaaactcgag gcgaaaccgt ggccctggcg gcgcccgctc cccctgccgc gatgatggcg
    11221 tttggagttc agactttgct tcatcggtgt gatacgaccc gaatgatggg gacgaaatgt
    11281 ccagcgcgtc acgcacgcat accgtatctc acggccaagc cttggatggc cggtcagcgc
    11341 gactcatgac agtcactgga tgctgtatgg ttccctgccg gcaagcgaca ccggaaccac
    11401 ctacaatatg gacactagcg tccaacaacg caatgtccaa gagttttttg gtttccgacc
    11461 ggcgccggcg cgcgggcgct gagacagcgc gcgatgccgg ccaggtcgag gtgccgcgcg
    11521 accgcgacca gcccggccag gcccagcagg cgcgccaccg cggccgcctg tccggcgccg
    11581 acctcgagca ccgcgacgcc gcccggcacc aacagccggc cgaggtcggg gaccagcgcg
    11641 cgataggcgt cgaggccgtc gccgccgccg tcgagcgcgc ggcgcggctc gtggcgcgcg
    11701 acctcggctg cgaggtgcgc gatggccgcg ctcggcacgt agggcgggtt ggcgacgacc
    11761 aggtcgaacc tcgcgcccac cgcccggccc cagtcgccga ccgcgaagcg cgcgcgcgca
    11821 tcgagcccca gccggcgcgc attcgcccgc gccagcgcca ccgcgcccgg atcgctgtcg
    11881 atgccgagcc cgcgcgcgcc cggccattcg ctgagcacgg cgagcagcag gcagcccgag
    11941 ccggtgccga ggtcgagcac ggtgccggcg cccgcgcccg cagcgaacgc cgccagcgcc
    12001 gcctcgacca ccgtctcgct gtcgggcctg ggcaccagca ccgcaggcgt gaccgcgaac
    12061 gagaggctcc agaactcgcg ctcgcccagg atataggcaa tcggctcgcg cgcgagacgg
    12121 cgcccgacca gggcctggaa gtccgccgcc gcatcaggca agagccggcg atcgggatcg
    12181 gcgatcaatt gcccgggcgc aaggccgctc gcatgggcga gcaacagccg cgcctcgcgc
    12241 cgcgcatcgg ttgcgggcaa cgcggcaagc cgcgccgcgg cccgggcgat cgcctcggcg
    12301 acggtcgccg gcggtcccgg ccgcgcgctc acgccagtgc cgccagctgg ctcgcctgat
    12361 cgtcggcgat cagggcgtcg atgaactcgt cgagcgcggc gccgtcgatc accgcgtcga
    12421 tgtcgtagta gctcatggcg atgcggtggt cggtcacccg gccctgcggg aagttgtagg
    12481 tgcggatgcg ttcggagcgg tcgccggagc cgacctgcgc gcgccggctc gcggcacgct
    12541 cggcctcgcg ccggctgcgc tcgcgctcgt acacgcgcgc gcgcaggatg cgcatcgcct
    12601 tggcgcggtt cttgtgctgc gacttctcgt cctgctgcga gaccacgatg ccggtcggca
    12661 gatgggtgat gcgcaccgcg ctgtcggtgg tgttcaccga ctgcccgccc gggccgctgg
    12721 agcggaacac gtcgatgcgc aggtccttgt cgtcgatgcg cacgtcgacc tcctcggcct
    12781 cgggcagcac cgccacggtc gccgccgagg tgtggacgcg cccctgcgcc tcggtgcgcg
    12841 gcacgcgctg gacgcggtgc acgcccgact cgtacttcag gcgcgcgaac acgctcctgc
    12901 ccttgatcgc ggcgaccgct tccttgaagc cgccgaggcc ggtctccgac aggtgcaggg
    12961 tctcgaaccg ccagcccttg atctcggcgt agcgctggta catgcggaac agggtggcgg
    13021 cgaacagcgc cgcctcctcg ccgccggtgc cggcgcggat ctcgaggatc gcgctcttct
    13081 cgtcggcctc gtcgcgcggc aacagcgcca ggcgcagcgc gtgatcgagc tcgccctcgc
    13141 gcgcccggca gccgtcgagc tcggcggcgg cgagcgcgcg catctcctcg ccgccggcgg
    13201 cgtccctggc cagctcggcg aggtcgcgga tctcgcgctt gagcttgcgc agcgcctcga
    13261 tcttggcgac caccggcgcg agctcggcgt actcgcgcgc gaggctgacg cgctgctcgc
    13321 tgctcggcgc gccgcccgcc atcagctcgc cgagctcgtg atgccgctcg acgatgcggt
    13381 ccaacttggc gtcaaggtcc atgtcgtgat gtcgtcgggc cggcgctggc cgcggccgcg
    13441 cgccggggct ccttcaggac gccaccggct cggccgcggg ctcgtcctcg tccgcctccg
    13501 cggcctcgat ctcggccgcc ttgcgctcga ccagctcgac caggtggtcg accaggcggg
    13561 cgtcgtcgat cttgtggtcg gtgacgccgg acagatagac ctgatgggtg cccttgccgc
    13621 cgccggtcag cccgatgtcg gtctcgcgcg cctcgcccgg tccgttgacc acgcagccga
    13681 tcaccgacag gctcagcggc gtcgagatgt gggcgaggcg ttgctcgagc agctcgacgg
    13741 tcctgatcac ctggaactgc tggcgggcgc aggacgggca ggcgatgatg ctgacgccgc
    13801 ggtggcgcag gctgagcgcc ttgagcagct cgaaaccgac cttgacctcc tcgaccggat
    13861 cggcggacag cgacacgcgc accgtgtcgc cgatccccga ccacagcagc atgccgagcc
    13921 cgatcgagga cttgaccgtg ccgccgcgca ggccgccggc ctcggtgatg ccgacgtgga
    13981 gcggatagtc gcacgcctcg gccaggccct gataggcggc caccgccagg aacacgtccg
    14041 aggccttgac gctgatcttg aactcgaaga agtcgttgtc ctcgaggatg cgcgcatggt
    14101 cgagggcgct ctcgaccatc gcctcggggc acggctcgcc gtacttctcc agcaggtcgc
    14161 gctcgagcga gccggcgttg acaccgatgc gcatcgagca gccgtggtcc ttggccgcct
    14221 tgaccacctc gcgcacccgc tcggcggcgc cgatgttgcc gggattgatg cgcaggcacg
    14281 ccgcgcccgc ttgggcggcc tcgatcgcgc gcttgtagtg gaaatggatg tcggcgacga
    14341 tcggcacgcc gacctggggc acgatccgct tcagcgccgc gctcgactcg gcgtcagggc
    14401 acgagatgcg gacgatgtcg gcgcccgccg cctcgagcgc ctgcacctgg gcgacggtgg
    14461 cggccacgtc ggtggtcggg gtgttggtca tcgactggac gctgatcggc gcctcgccgc
    14521 cgaccgcgac cgcaccgaca cggatctggc ggctcttgcg ccgggcgatg tcgcgataag
    14581 ggcggacgct catcagctgg actcgatctc gaacctcggc cccggcggcc accccggggg
    14641 cggcgggact atagccaatc gggcggggcg gcgaaagcca cgcggcgggc ggctcagcgc
    14701 tggccgaccg tgccgccgct ggccagcgcc tcgggcgtca gccggatcgc gcgccgcacc
    14761 gagccttcgg tgcccaaggg cggcgccggc acgccgtcga ccagcacttc gagcccgccg
    14821 gcattgccgg tgaccagggt caggtcgtcg cggtcgggaa ccaggaacac gtcgcccggg
    14881 cgcaggatgc gggtcaggat caggtcgttg ttggcgccct gcacctgcac ccaggcgtcg
    14941 gcgcgggcgc ggatcacgat ccgggcatcg gcattgccct ggccgtagac gcgcggctcg
    15001 taaccggcgg cgggtgccgc cggcggcggc ggcgggtcct cgatcaggtc gggcgcgtcc
    15061 ggggtcgcgg ccagcgcgcc ggtgatcacg gtcgccgacc cggggcggct gtcggcgccg
    15121 aacggcgagg cgc
//
LOCUS       DASWHU010000211         4995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1593392, whole genome shotgun sequence.
ACCESSION   DASWHU010000211 DASWHU010000000
VERSION     DASWHU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593392"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1440
                     /gene="tyrS"
                     /locus_tag="VGB88_07770"
     CDS             187..1440
                     /gene="tyrS"
                     /locus_tag="VGB88_07770"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9647380.1"
                     /translation="MTQVTSPFLRSVVDRGYLHQCTDLEGLDSLAAARSIVAYIGFDA
                     TADSLHVGSLVPIMLLRRLQQAGHKPIVLMGGGTTRVGDPSGRDASRRLLSDKDIARN
                     IAGIRRTFERFLAFGDGAAEAVMIDNAAWLNELNYLDFLRDYGSHFSVNRMLGFESVK
                     LRLEREQTLSFLEFNYMVLQAYDFVELARRHDCVLQMGGSDQWGNIVCGVELARRIDQ
                     RTLYGLTAPLITTASGAKMGKTAAGAVWLNPERLSAYGYWQYWRNTEDADVGRFLRLF
                     TDLALDEIARLEALRGAEINDAKKVLATEATRLAHGEDAALAAARTAAQTFEQGELGE
                     ALPEVAVPSAELEGGVAAFRLFSLAGLCASNGEARRLIRGGGARINDVLVEAEDRPVG
                     IGDLTADGVIKLSAGRKRHALVRPS"
     gene            complement(1655..1978)
                     /gene="fliN"
                     /locus_tag="VGB88_07775"
     CDS             complement(1655..1978)
                     /gene="fliN"
                     /locus_tag="VGB88_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX9647381.1"
                     /translation="MAEEAQPDDGAGEGEGAPEPAATAPGNGEADRALNEVPVEISAV
                     LGTARVPVAQLLKMGRGAVLELDRKVGDPIEIFVNKRMVARGEVTVLDDRLGVTLSET
                     IKLAR"
     gene            complement(2065..>4995)
                     /locus_tag="VGB88_07780"
     CDS             complement(2065..>4995)
                     /locus_tag="VGB88_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEX9647382.1"
                     /translation="TWAGWERAIDIRARGVRAIATDGGALAGVPEMAIGLSLPALARG
                     LIAPTRLDVFGAGVRLVRTADGRLDLDFGAEGDGRQRGIAEALLQMFVLSPEPGSAAS
                     YLSELSIVDSRLVIRDQRLGVEWTAPAARLKIERRTGGLLAGAILKLEVADQLVDVTA
                     SADYDSGGRLAKVGLTFSELNPAKLAGLADALAPLARFDLPVGGRLDLVGSGDGSLRE
                     VAFDLVGGAGRIALSDVLGIDAGRDVALVQARGVLTDGLARLAMDELYIDFGGPTATM
                     AGQAVGDWRAPELTAEVELRGVPVAEIGRYWPDDVAAGARAWVGERMTEGVVDRLALR
                     LAVNPGDWNAPRLARDQIDARFEIRGATVAYSPTLPPVTGVDAGGRFDGGALHAEIRS
                     GASGPLEARAGTVELQGYDAGRGRAEVSVAFSGSLPETLAGLARTPFAASYLQGINVD
                     AVSGRAEGDIAVALPMVPILPEGAVRVSVGADLHALDIEPGGLSMLAAGVAISGGEAA
                     FLLDGSGFDLTGEAQLDGTPASFFWHESFAAEADIRRRIDVTARLDDAARARLGFADQ
                     KISGPIDVELRINDLRDGSRVGELDVGATQAQLALPVLDWRKPPGEPASARFALELDG
                     SGIARVPRFGVQAEGLSAAGSARRDGAGLWTLAFDRLVAGSTDVRGEVRVRADQGYDV
                     AVYGRRLDLRRYFAGAGDEPAGAARAFNLIANVDTVLLGEALVLHKVEARSRFDGAHV
                     TDAAFTCDLGRAKDVAFSLEGGAPGQRRFSVAADDAGAVFEALDVTPNLIGGALSVEG
                     VIDDTGADAPLAGTIRVSDFHMVDAPVLAKLLNVVSITGALEMLKGSGIPFDRLEAQF
                     AYRDDVLTLKEGLARGLSIGLTLEGEVDLARDWIDLAGTIVPAYFLNTALGRIPVIGD
                     ILTGGEGEGVFAATFVAEGPRQAPSVFVNPLAVLAPGILRDLISKMPLGTPLEPWPGG
                     RSAH"
BASE COUNT          690 a         1869 c         1765 g          671 t
ORIGIN      
        1 ccgcctcgcc gtcggtgcgc agcagcgcgg catcgacgcc gtcggccgag gtaccgctca
       61 tcagcccgag cgcgagcacg gcggcaccgc tcacccgctg cctgccgcgc ccggcgcagc
      121 ctccgttgcc gcgcgcgccc gcgtcatgtt aagcaaccgg cccgccccac gcgcccccgc
      181 caggccatga cccaggtcac gtcgcccttc ctgcgctccg tcgtcgaccg cggctacctg
      241 catcagtgca ccgacctcga ggggctcgat tcgctcgccg ccgcccgctc catcgtcgcc
      301 tatatcggct tcgacgccac cgccgacagc ctgcatgtgg gctcgctggt gccgatcatg
      361 ctgctcaggc gcctgcagca ggccggccac aagccgatcg tgctgatggg cggcggcacc
      421 acccgcgtcg gcgacccgtc cgggcgcgac gccagccgca ggctgctgtc ggacaaggac
      481 atcgcccgca acatcgccgg catccggcgc accttcgagc gcttcctcgc cttcggcgac
      541 ggcgcggcgg aggcggtgat gatcgacaac gcggcctggc tcaacgagct caactatctc
      601 gatttcctcc gcgactacgg cagccacttc tcggtcaacc gcatgctcgg cttcgagtcg
      661 gtcaagctca ggctcgagcg cgagcagacc ttgagcttcc tcgagttcaa ctacatggtg
      721 ctccaggcct acgacttcgt cgagctggcc cggcgccacg actgcgtcct ccagatgggc
      781 ggctcggacc agtggggcaa catcgtctgc ggcgtcgagc tcgcccggcg catcgaccag
      841 cgcacgctgt acggactgac cgcgccgctg atcaccacag cgtccggcgc caagatgggc
      901 aagaccgcgg ccggggcggt gtggctcaac cccgagcgcc tgtcggccta cggctactgg
      961 cagtactggc gcaacaccga ggacgccgac gtcggccgct tcctgcgcct gttcaccgac
     1021 ctcgcgctcg acgagatcgc gcggctcgag gcgctccggg gcgccgagat caacgacgcc
     1081 aagaaggtgc tggcgaccga ggcgacgcgg ctcgcccacg gcgaggacgc cgcgctcgcc
     1141 gcggcgcgca ccgcggcgca gaccttcgag cagggcgagc tcggcgaggc cttgcccgag
     1201 gtcgcggtgc cgagcgccga gctcgagggg ggcgtcgccg ccttccggct gttctcgctc
     1261 gccgggctgt gcgcgagcaa cggcgaggcg cggcgcctga tccgcggcgg cggcgcgcgc
     1321 atcaacgacg tcctggtcga ggccgaggac cgtccggtcg gcatcggcga cctgaccgcc
     1381 gacggggtga tcaagctgtc ggcgggccgc aagcgccacg cgctggtgcg cccgtcctga
     1441 cagctttcga agctgtcgcc ggacggttcc gtcttggtca ttcccgcgaa aaaggctgtg
     1501 aagaaatcgc catccggtcc cttcatcgtc attcccgcgg cagcgggaat ccagattctt
     1561 caatgagtta ctggaccccc gcttgcgcgc gggtgacgac atgaggcggg gcggtggagt
     1621 ccgtcacgag cccttgctcg gccggtcgcc ggcgtcagcg ggcgagcttg atggtctcgc
     1681 tcaaggtcac gccgaggcgg tcgtcgagca cggtgacctc gccgcgcgcg accatgcgct
     1741 tgttgacgaa gatctcgatc ggatcgccga ccttgcggtc gagctcgagc accgcgccgc
     1801 gtcccatctt caacagctgc gcgaccggca cccgggcggt gccgagcacc gcggagatct
     1861 cgaccggcac ctcgttgagc gcgcggtcgg cctcgccatt gccgggcgcc gtggcggcgg
     1921 gctcgggcgc gccctcgccc tcgcccgcgc cgtcgtccgg ttgcgcctcc tccgccatca
     1981 cgctgcccct cggtcaactg ccgccggggc catggtaatg cgcactggtt aacggaatct
     2041 tagcggccgt ggccggagtg cgcgtcagtg cgcgctgcgc ccgcccggcc agggctcgag
     2101 aggggttccg agcggcatct tgctgatcag gtcgcgcagg atgccgggcg cgagcacggc
     2161 aagcgggttg acgaacacgc tcggcgcctg gcgcgggccc tcggcgacga aggtggcggc
     2221 gaacacgccc tcgccctcgc cgccggtcag gatgtcgccg atcaccggaa tccggccgag
     2281 cgcggtgttg aggaaatagg cgggcacgat ggtgccggcg aggtcgatcc agtcgcgcgc
     2341 caggtctacc tcgccctcca gggtgagccc gatcgacagg ccgcgcgcca gcccctcttt
     2401 gagcgtcagc acgtcgtccc ggtaggcgaa ctgcgcctcg agccggtcga acgggatgcc
     2461 gctgcccttg agcatctcga gcgcgccggt gatcgagacc acgttcagca gcttcgccag
     2521 caccggcgca tcgaccatgt ggaagtcgct gacccggatc gtgccggcga gcggcgcgtc
     2581 cgcgcccgtg tcgtcgatca cgccctcgac cgacagcgcg ccgccgatca ggttgggcgt
     2641 gacgtcgagc gcctcgaaca ccgcgccggc atcgtcggcg gcgaccgaga accggcgctg
     2701 gcccggcgcg ccgccctcga ggctgaacgc gacgtccttg gcgcggccga ggtcgcaggt
     2761 gaaggcggcg tcggtgacat gggcgccgtc gaagcgggaa cgcgcctcga ccttgtgcaa
     2821 caccagcgcc tcgcccagca gcacggtatc gacgttggcg atcaggttga acgcccgcgc
     2881 tgcgcccgcc ggctcgtcgc cggcgccggc gaagtaccgg cgcaggtcga gtctccggcc
     2941 atagacggcc acgtcatagc cttggtccgc acgcacccgg acctcgccgc gcacgtcggt
     3001 cgagccggcc accagccgat cgaaggcgag cgtccacagc ccggcgccgt cgcggcgcgc
     3061 ggagccggcg gcggacaagc cctcggcctg cacgccgaag cgcggaacgc gcgcgattcc
     3121 ggaaccgtcc agctccagcg cgaagcgcgc cgacgccggc tcgcccggcg gcttccgcca
     3181 gtcgagcacc ggcaaggcga gctgcgcctg ggttgcgccg acgtccagct ctccgactcg
     3241 gcttccgtcg cgcaggtcgt tgatacgtag ctcgacatct attggcccgc ttatcttctg
     3301 gtcggcgaag ccgagccgcg cgcgggcggc gtcgtcgagc cgcgcggtga cgtcgatccg
     3361 gcgcctgatg tcggcctccg cggcgaagga ttcgtgccag aagaacgagg ccggcgttcc
     3421 gtcgagctgc gcctcgccgg tcaggtcgaa gccgctgccg tcgagcagga acgccgcctc
     3481 gccgcctgag atggcgacgc ccgccgccag catgctgagg ccgccgggct cgatgtcgag
     3541 cgcgtgcagg tcggcgccca ccgagacgcg cacggcgccc tcggggagga tcggcaccat
     3601 cggcagggcg acggcgatgt cgccttcggc gcggccgctc accgcatcga cgttgatgcc
     3661 ttgcaggtac gaggccgcaa acggggttcg ggcgagcccg gccagcgtct ctggcagcga
     3721 cccggaaaag gcgaccgaga cctcggcgcg cccgcgcccg gcgtcgtagc cctgtagctc
     3781 gaccgtgccg gcgcgggcct cgagcgggcc ggaggcgccg gaccggatct cggcatgcag
     3841 cgcgccgccg tcgaagcgcc cgccggcatc gaccccggtc accggcggca gggtggggct
     3901 gtaggcgacg gtcgcgcccc tgatctcgaa ccgggcgtcg atctggtcgc gcgccagccg
     3961 cggcgcgttc cagtcgcccg ggttgaccgc cagcctgagc gccaggcggt cgacgacgcc
     4021 ctcggtcatc cgttcgccga cccaggcgcg cgcgccggcg gcaacgtcgt ccggccagta
     4081 gcggccgatc tcggcgaccg gcacgccgcg gagctcgacc tccgcggtca gctcgggcgc
     4141 ccgccagtcg ccgacggcct ggccggccat cgtcgcggtc gggccgccga agtcgatata
     4201 gagctcgtcc atcgccagcc gggcgagccc gtcggtgagc acgccgcgcg cctgcaccag
     4261 cgcgacgtcg cgcccggcgt cgatgccgag cacgtccgac agcgcgatcc tgccggcgcc
     4321 gcccaccagg tcgaaggcga cctcgcgcag gctgccgtcg ccgcttccga ccaggtcgag
     4381 gcggccgccg accggcaggt cgaaccgcgc caacggcgcc agcgcatcgg ccaggccggc
     4441 gagcttcgcc gggttcagct cggagaaggt caggccgacc ttggccaggc ggccgccgct
     4501 gtcgtagtcg gcgctggcgg tgacgtcgac cagctggtcg gcgacctcga gcttcaggat
     4561 cgcgccggcg agcagtccgc cggtgcggcg ctcgatcttc agccgcgcgg cgggcgcggt
     4621 ccattccacg cccagccttt ggtcgcggat gaccagccgc gaatcgacga tgctgagctc
     4681 gctgaggtag ctggcggcac tgcccggctc gggcgacagc acgaacatct gcaacagcgc
     4741 ctcggcgatg ccccgctggc ggccgtcgcc ctcggcgccg aagtcgaggt cgaggcggcc
     4801 gtccgcggtg cgtaccaggc gcaccccggc accaaagacg tcgagccggg tcggcgcgat
     4861 caggccgcgg gcgagcgccg gcaggctgag cccgatcgcc atctcgggca cgccggcaag
     4921 cgcgccgccg tcggtggcaa tcgcgcgcac gccgcgcgcg cggatgtcga tggcgcgctc
     4981 ccagccggcc caggt
//
LOCUS       DASWHU010000212         4762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1971217, whole genome shotgun sequence.
ACCESSION   DASWHU010000212 DASWHU010000000
VERSION     DASWHU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971217"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VGB88_07785"
     CDS             <1..488
                     /locus_tag="VGB88_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647383.1"
                     /translation="SGRTPMVCGLGTELAFKKRKVRYLSFDTLIEISDQYRTRPPDAA
                     LPPIGPWGPKNIFYWPWFEAETLIIDDISPIIGAAMREFGPDTFEKILTTGLAGAAAE
                     LRSRDTIWVFGVESADISFKLARPGEEIALRYARDIQAFCHAESPPLVVRLPPAETRM
                     E"
     gene            590..3436
                     /locus_tag="VGB88_07790"
     CDS             590..3436
                     /locus_tag="VGB88_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9647384.1"
                     /translation="MSVTAPQTDAAPAIAGEDMEARVLALVRGLARELHPERADVAAL
                     TLDAALDRDFGIDSLARAELLARLERAFAVGLAEDAVMDAETARDLLAAIARARPPAR
                     ASLPAAPLATGEAEVVPRAAATLLEVLAFHRASHAARVHVRLVGTEAAERALTYDELA
                     QGAEAIAAGLLEAEVAPGEIVALMLPTGGDFFRCFLGVLIAGCVPAPLYPPARRAHVA
                     EHLLRQAAIVRNARAPLLVTTDEALRFGHLLQAQAESLRHVVTPEALARPGAAPPWPR
                     LAPTAPALVQYTSGSTGDPKGVVLSHANLLANIHALAGALDAGPHDVVVNWLPLYHDM
                     GLIGHWLGSLTYACPMVCMPPLHFLARPERWLRAISDHGGTLSAAPNFAYELCAARVR
                     EGALDGLDLSSWRMAGCGAEAVSASTIARFAARFARWGFRPSAMTPMYGLAESAVAVT
                     VSPLGRGPRIDRVRRDSLARGNRAEPAGGAEPAVEVVGCGLPIAGHQVRIVGAAGAEA
                     GEREVGRIEFQGPSCTAGYLRAAAKTAALFHGAWLESGDLGYIADGELFVTGRAKDVI
                     VRAGRNLYPEEIEAAVGALAEVRTGRVAAFAAPDPATGSERLIVLAETRLTDSGRRAA
                     LAARVRELVAELAELPPDEVVLARPGTVLKTNNGKLRRAACRRLYEAGRLDRPPPAPW
                     RQALAVVQASLGPAWRRARAAAAALAYAAWFQAVYRGLAPVVWLLVVALPATGARRRL
                     LRSAARLMMRALGLGPAAVGLANLPRMGAHVIAANHASYLDSLALIALLPPRYGFVAK
                     RELGEGAVSGLFLRRLGALFVDRLDPRAGPEDLARMVAALGAGAALVVYPEGTFDRRP
                     GLRPFHLGAFAAAAKAGVPVVPVAIRGSRAVLRGDSRFARRGRITVTVHQPIAPAGAD
                     WAEALRLRDLVRAALLRSCGEPDLIFEPARVPDR"
     gene            3525..3872
                     /locus_tag="VGB88_07795"
     CDS             3525..3872
                     /locus_tag="VGB88_07795"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9647385.1"
                     /translation="MKLCERMLPAVAATLAFAGTEALAQDEERYRYVYGHPSMMGDWG
                     WGGMIFGPLLGLLYIGLIVVAVIFVVRWLGGELGHRPGGRARPALQILEERFARGEID
                     QEEFEQRKRLLSE"
     gene            complement(3877..>4762)
                     /locus_tag="VGB88_07800"
     CDS             complement(3877..>4762)
                     /locus_tag="VGB88_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9647386.1"
                     /translation="AAGGTLGILIPPSIILVIYAVLTEQNIVKLFLAATVPGLLAVLG
                     FMVAIALYVRLFPDEGPAGARLPMRARLRALKRVWQVVAIFAIAIGGIYGGYFTPSEG
                     ASVGVVLMAALALALGALTPRAMLAAAVATASASAMIFAILFGADLFNVALALTRIPV
                     LAADWVGASSLAPWLVLAVLIALYLALGCVMDSLSMILLTVPTIFPAFMALDFGLEAT
                     HQALWFGVLTLIVVELGMITPPVGLNLFVISSLADGVPTRAVFRGAVPFILSEVVRVA
                     LIVAFPALTYWLVDLVAG"
BASE COUNT          606 a         1737 c         1784 g          635 t
ORIGIN      
        1 cctcggggcg cacgcccatg gtgtgcggcc tcggcaccga gctcgcgttc aagaagcgca
       61 aggtgcgcta cctgtcgttc gacacgctga tcgagatctc ggaccagtac cgcacccggc
      121 cgcccgacgc ggcgctgccg ccgatcggcc cgtgggggcc caagaacatc ttctactggc
      181 cgtggttcga ggccgagacc ctgatcatcg acgacatcag cccgatcatc ggcgcggcga
      241 tgcgcgagtt cggccccgac accttcgaga agatcctcac caccgggctc gccggggcgg
      301 ccgctgagct gaggtcgcgc gacaccatct gggtgttcgg cgtcgaaagc gccgacatat
      361 cgttcaagct ggcgcggccg ggcgaggaga tcgccttgcg ctacgctcgc gacatccagg
      421 cgttctgcca cgcggagtcg ccgccgctgg tggtgcggct gcccccggcc gagacccgca
      481 tggagtgacc ggaccggcgc cggcggcgcg cgccgaccgg ccggttcggg ttgtgcgcgc
      541 gcgcacgcct ctgccatgct cgcccggtga gcgacggccg ggccaagcca tgtcggtgac
      601 cgcaccgcag acggacgccg cgcccgccat cgcgggcgag gacatggagg cgcgcgtgct
      661 cgcgctggtg cgcggcctcg cgcgcgagct gcacccggag cgcgccgatg tcgccgcgct
      721 caccctcgac gccgcgctcg accgcgactt cggcatcgac agcctggccc gcgccgagct
      781 gctggcgcga ctcgagcgcg ctttcgccgt cggcctggcg gaggacgcgg tgatggacgc
      841 ggagaccgcg cgcgacctgc tggccgcgat cgcgcgcgcg cgccctcccg cgcgcgcgtc
      901 gctgccggcg gcgccgcttg cgaccggcga ggccgaggtg gtgccgcgcg cggcggcgac
      961 cctgctcgag gtgcttgcct ttcaccgtgc cagccatgct gcgcgcgtgc acgtgcgcct
     1021 ggtcggcacc gaggcggccg agcgcgcgct gacctacgac gagctcgcgc aaggcgccga
     1081 ggcgatcgcc gcgggcctgc tcgaggccga ggtcgcgccg ggcgagatcg tcgcgctgat
     1141 gctgccgacc gggggcgact tcttccgctg cttcctcggc gtgctgattg ccggctgcgt
     1201 gccggcgccg ctctatccgc cggccaggcg cgcgcatgtc gcggagcacc tgctgcgcca
     1261 ggcggcaatc gtgagaaacg cgcgcgcgcc actgctggtg accaccgacg aggcgctccg
     1321 cttcggccac ctgttgcagg cgcaggccga gagcttgcgc catgtggtga cgccggaggc
     1381 gctggcccgc cccggcgccg ccccgccgtg gccgcgcctc gccccgacgg cgccggcgct
     1441 ggtccagtac acctcgggca gcaccggcga ccccaagggc gtggtgctga gccatgccaa
     1501 cctgctcgcc aacatccatg cgctggcggg cgcgctcgac gccgggccgc acgacgtggt
     1561 ggtcaactgg ctgccgctct accacgacat ggggctgatc gggcactggc tcggcagcct
     1621 cacctatgct tgcccgatgg tgtgcatgcc gccgcttcat ttcctggcca ggcccgagcg
     1681 ctggctgcgg gcgatctcgg accacggcgg caccttgtcg gccgcgccca acttcgccta
     1741 cgagctgtgc gccgcgcgcg tgcgcgaggg ggcgctcgac ggcctcgacc tgtcgagctg
     1801 gcgcatggcc gggtgcggcg ccgaggcggt gagcgcctcg accatcgcgc gctttgccgc
     1861 acgcttcgcg cgctggggct tcaggccttc ggcgatgacg ccgatgtacg gcctcgccga
     1921 gagcgcggtc gcggtcaccg tctcgcctct cggccgcggc ccgcgcatcg accgcgtccg
     1981 ccgcgattcg ctcgcccgcg gcaaccgcgc cgagcccgcc ggcggcgcgg agccggcggt
     2041 cgaggtggtc ggctgcgggc tgccgatcgc cggccaccag gtgcgcatcg tcggcgccgc
     2101 cggcgccgag gccggcgagc gcgaggtcgg ccggatcgag ttccaggggc cgtcctgcac
     2161 cgccggctat ctgcgcgctg cggccaagac cgcggcgctg ttccacggcg cctggctgga
     2221 gtcgggcgac ttgggctaca tcgccgacgg cgagctgttc gtcaccgggc gcgccaagga
     2281 cgtgatcgtg cgcgccgggc gcaacctgta ccccgaggag atcgaggccg cggtcggcgc
     2341 gctggcggag gtgcgcacgg gccgggtggc ggcgttcgcc gcgcccgatc ccgcgaccgg
     2401 cagcgagcgg ctgatcgtgc tcgccgagac gcggctcacc gattcgggcc ggcgcgccgc
     2461 gctcgccgcg cgggtgcgcg agctggtcgc cgagctcgcc gagctgccgc cggacgaggt
     2521 ggtgctggcg cggcccggca cggtgctcaa gaccaacaac ggcaagctcc gccgcgccgc
     2581 ctgccggcgg ctctacgagg ccggacggct cgatcgcccg ccgccggcgc cgtggcgcca
     2641 ggcgctcgcc gtcgtccagg cgagccttgg cccggcctgg cggcgggcgc gggccgcggc
     2701 cgccgcgctc gcctatgcgg cgtggttcca ggcggtctat cgcggtcttg cgccggtggt
     2761 gtggctgctg gtggtggcgc tgcccgcgac tggggcgagg cgccggctgc tgcggtcggc
     2821 ggcgcggctg atgatgcgcg ccctcggcct cggcccggcg gcggtcggcc ttgccaacct
     2881 gccgcggatg ggcgcccacg tgatcgcggc caatcacgcg agctatctcg attcgttggc
     2941 gctgatcgcc ctgctgccgc cgcgctacgg cttcgtcgcc aagcgcgagc tcggcgaggg
     3001 ggcggtctcg gggctgttcc tgcgccggct cggcgcgctg ttcgtcgacc ggctcgatcc
     3061 gcgcgcgggg cccgaggacc ttgcccggat ggtggcggcg ctcggcgccg gcgcggcgct
     3121 ggtggtctat cccgagggca ccttcgaccg ccggccgggc ctgcgcccgt tccacctcgg
     3181 tgccttcgcc gccgccgcca aggcgggcgt gccggtggtg ccggtcgcca tccgcggctc
     3241 gcgcgcggtg ctgcgcggcg acagccgctt cgcccgccgc ggccggatca cggtgaccgt
     3301 gcaccagccg atcgcgcccg cgggcgcgga ctgggccgag gccttgcggc tccgcgacct
     3361 cgtccgggcg gcgctgctca gaagctgcgg cgagcccgac ctgatcttcg agcccgcgcg
     3421 cgtcccggac cgatgaccgc ctgcgaacag cttgatcggc ccccgcccga tgccgctata
     3481 atggccgggc tcctgcggcg aggatcgacg aggacggtga tgccatgaag ctctgcgagc
     3541 ggatgctgcc ggcggttgcg gcgacactgg catttgccgg gaccgaggct ttggcgcagg
     3601 atgaggaacg ctaccgctac gtatatggac atccatccat gatgggcgat tggggctggg
     3661 gcggcatgat cttcggtccg ttgctgggac tgctctatat cgggctgatc gtggtcgcgg
     3721 tgatcttcgt cgtgcgctgg ctgggcggcg agctcggcca ccggccgggg gggcgcgcgc
     3781 gcccggcgct gcagatcctc gaggagcgct tcgcccgcgg cgagatcgac caggaggagt
     3841 tcgagcagcg caagcgcctg ctgtccgagt gaccgctcag ccggcgacca ggtcgaccag
     3901 ccaataggtc agcgccggaa aggcgacgat cagcgcgacg cgcaccacct cggacaggat
     3961 gaacggcacc gcgccccgga acaccgcgcg cgtcggcacg ccgtccgcga ggctcgagat
     4021 cacgaacagg ttgagcccga ccggcggggt gatcatgccg agctcgacca cgatcagggt
     4081 gagcacgccg aaccacagcg cctggtgggt cgcctccagg ccgaagtcga gcgccatgaa
     4141 cgccgggaag atggtcggca ccgtcaacag gatcatcgac agcgagtcca tgacgcagcc
     4201 gagcgcgaga tagagcgcga tcagcaccgc gagcaccagc cacggtgcca ggctcgaggc
     4261 gcccacccag tcggcggcga gcaccgggat gcgggtgagc gcgagcgcga cgttgaacag
     4321 gtcggcgccg aacaggatgg cgaagatcat cgcgctcgcc gacgcggtgg cgaccgccgc
     4381 ggcgagcatc gcccggggcg tcagcgcgcc gagcgcgagc gcgagcgccg ccatcagcac
     4441 cacccccacc gaggcgccct ccgagggcgt gaagtagccg ccgtagatgc cgccgatggc
     4501 gatcgcgaag atcgccacca cctgccacac gcgtttcagc gcacgcaggc gcgcgcgcat
     4561 gggaaggcgt gcgccggccg gcccttcgtc gggaaacagg cgcacgtaga gcgcgatcgc
     4621 aaccatgaag ccgagcaccg cgagcagccc cggcacggtc gcggcgagga acagcttgac
     4681 gatgttctgc tcggtgagca cggcgtagat caccaggatg atcgacggcg ggatcaggat
     4741 gccgagggtg ccgccggcgg cg
//
LOCUS       DASWHU010000213        14111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_102289, whole genome shotgun sequence.
ACCESSION   DASWHU010000213 DASWHU010000000
VERSION     DASWHU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102289"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VGB88_07805"
     CDS             <1..141
                     /locus_tag="VGB88_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX9647387.1"
                     /translation="SDVATTLEGLGRELGISKERVRQIEARAMAKLRRILSQRVGEQA
                     TV"
     gene            complement(155..982)
                     /gene="proC"
                     /locus_tag="VGB88_07810"
     CDS             complement(155..982)
                     /gene="proC"
                     /locus_tag="VGB88_07810"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX9647388.1"
                     /translation="MAARVVLVGGGKMGGALLAGWLGKGGIAAGEVVVVEPAADAARA
                     LGRHGVTVIDSPAALDGGQAPAVVVFAVKPQVIAGVVADYRRPAGAKGPAPVYLSIAA
                     GTRLALFEQRLGAQAAVVRAMPNTPAAVGRGITVAVANARVGAEQIALCTELLEAVGE
                     VAWIGDEALMDAVTGLSGSGPAYVFLMIECLAAAGVAVGLAPDLAARLARATVTGAGE
                     LAWRSEDSAETLRRNVTSPGGTTAAALDVLMAADGLEALMTRAVAAATERSRELASG"
     gene            complement(986..1489)
                     /locus_tag="VGB88_07815"
     CDS             complement(986..1489)
                     /locus_tag="VGB88_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX9647389.1"
                     /translation="MARIAFLELEPAGNPIDLVEELVAANDWNFERQGENELAVQMPG
                     QWCDIHMWFARREETRSMIFTCALDMRVPAERRQAVYPLLAKINQRLWVGHFELWADE
                     GWPTFRHTVIAGSEGKISAAVLDDLIDAARSECDRFYPAFQYVIWAGKDADQAIAAAL
                     VDPVGEA"
     gene            complement(1665..1991)
                     /locus_tag="VGB88_07820"
     CDS             complement(1665..1991)
                     /locus_tag="VGB88_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX9647390.1"
                     /translation="MERDSKLLDDLSRMAGGAMSSLAALREEAEARLRAHAERLAANM
                     DLVRRDEFEAVQALAATAREQAEVLAERLAALEQRLAVLEAGARRRAAGKGAAKDAGK
                     AEDNKA"
     gene            2097..3101
                     /gene="rfaD"
                     /locus_tag="VGB88_07825"
     CDS             2097..3101
                     /gene="rfaD"
                     /locus_tag="VGB88_07825"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX9647391.1"
                     /translation="MIVVTGGAGFIGSNLVAALAEREIFEVVVCDTFGDGDKWRNLAR
                     HEVADIVAPEHLFRFLINRARRGDGWGRVEVVVHLGANSSTTETDVDAMLRQNFGFSM
                     TLWRLCTEHRVRLIYASSAATYGDGGAGFDDDARADALARLRPLNPYGWSKHLFDRRA
                     VRLAASADTRPPQWVGLKFFNVYGPNEYHKGAMQSVVAQKFPLARAGKPVTLFRSCDP
                     DYADGGQMRDFVYVRDCVEVMMWLLDHAEVCGLFNVGTGRARSFLDLARALQRALGAD
                     ERIEFIDMPQSLRAQYQYFTEARIDRLRRAGYDAPFTTLEAGVADYVANYLATDDPYR
                     "
     gene            3098..3925
                     /gene="lgt"
                     /locus_tag="VGB88_07830"
     CDS             3098..3925
                     /gene="lgt"
                     /locus_tag="VGB88_07830"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9647392.1"
                     /translation="MSGVLAFAALSFPDIDPVAIQLGPVAIRWYALAYIAGLALGWGY
                     VRRLAQTGVAAFTKAEVDDLLFWVALGVILGGRLGYALFYRADHYLSNPLELVMLWRG
                     GMSFHGGLLGVVVALAWFARHRRRPLLAVADAVAVAIPIGLLFGRLANFINGELYGRI
                     ADADLPWSMIFPTGGPFPRHPSQLYEAGLEGLALFAVLAWLVHRQGALDRRGLVTGTF
                     LVGYSIARAAGELFREPDAHIGFLIGGMTMGQFLSIPMLLFGAWLILRARPAARNAG"
     gene            3922..5013
                     /locus_tag="VGB88_07835"
     CDS             3922..5013
                     /locus_tag="VGB88_07835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9647393.1"
                     /translation="MTALGARIRRRIRLGGPITLAQYMAEALANPADGYYGGEAGGAV
                     IGARGDFVTAPEISQMFGELIGLWCAEMWRRMGAPDPVRLVELGPGRGTLMADALRAA
                     ATVPALRAALRIHLVETSPRLRARQAETLAGSGADWHDSLADAPDGPLLLVANEFFDA
                     LPIHQFTATAAGWRERLVGVAGDGRLALCAAPGATPALALLDRLGVEPVPGAVAELCP
                     AGIALAATIGARVAAERGAALIIDYGAGGALGGSVQAVRAHRVVAPLAEPGAADLSAH
                     VDFAALARAAREAGAAVHGPVAQGAFLRALGLEARAARLARRATPAQQAALAAACARL
                     AGDDQMGALYKVLAIAHPALPTPAGFGEE"
     gene            5010..5798
                     /gene="pgeF"
                     /locus_tag="VGB88_07840"
     CDS             5010..5798
                     /gene="pgeF"
                     /locus_tag="VGB88_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX9647394.1"
                     /translation="MSARALTDAGLAAAPGVAHGFFTRRGGVSAGVYGSLNCGLGSGD
                     AGAAVAENRARAMAALRLGPEALVTGYQVHGIEVRIIDGNETAGERRARRHTDAAVTS
                     APGIALGVLTADCAPVLLADPEARVIGAAHAGWRGALAGVVEATVAAMERLGASRGAI
                     VAAIGPCIAAESYEVGPEFPAPFLADDAASARFFAPRRSGGRLSFDLAGYLAWRLGRL
                     GLGEVGATGGDTCADDAAFFSYRRAYRRGETDYGRLLSAIALES"
     gene            5811..6692
                     /locus_tag="VGB88_07845"
     CDS             5811..6692
                     /locus_tag="VGB88_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647395.1"
                     /translation="MPYLVWFVTLLIVALVASCVLAACQPVPRPFQPETKSAADFAGF
                     TGVADKLGIFVAGIDNAPAGLDRAVVGALVEGLHSRDIAASDRVANRASYLLIGTARP
                     DAPDGLVIAWRLIDSDGLVIGLVDQHLGVDDAAWRRGDPALAAAIADQAAPRLAAVIA
                     DAAPAGPAPRLPSPGAVILAEVEGARGDGDAELTRAMRAALERLGIAVVDTPGAAHPI
                     VRGQVLRRPRGADELVEIRWSVEAPDGGELGRIGQSNLVPAGSLDGDWGPVARAVADG
                     AAEGVRALLDELAHAPK"
     gene            6815..7747
                     /locus_tag="VGB88_07850"
     CDS             6815..7747
                     /locus_tag="VGB88_07850"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9647396.1"
                     /translation="MKIVAGNSNRPLAEAVAAFCNLPLVNASIRRFSDQEVFVEIHEN
                     VRGEDMFLVQSTSYPANDNLMELLVTLDALRRASARRITAVVPYFGYARQDRKPGPRT
                     PISAKLVANLITVAGADRVLTMDLHAGQIQGFFDIPVDNLYAAPVMIADIKQNFSGDK
                     LVVVSPDVGGVVRARALAKRIDADLAIVDKRRERAGVSEVVNIIGEVDGRRCLLVDDI
                     VDSAGTLCNAAVALKDNGATTVMAYVTHGVLSGGAVARVASSELEELVITDTIQATEA
                     VRVANNVRMLSISQLMAEAMRRISEESSVSSLFD"
     gene            7784..8476
                     /locus_tag="VGB88_07855"
     CDS             7784..8476
                     /locus_tag="VGB88_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9647397.1"
                     /translation="MADSLIISAEPRAAAGKGAARATRRAGRVPAVVYGRGEQPIAVS
                     VDRHELAQEYRRGGFFSRLYDLKLGEENVRVLPREVQSDPVTDAPLHVDFLRLAADSR
                     IDVDVPVMFINDLESPGLRRGGVLNVVRHVVELNCRADSIPESITVDLTGLQIGDSVH
                     ISAIALPENVRTTIAGRDFTIATVAAPTVVAEEAAAEAAEAAEGEAAVPEGEAAAPEA
                     GEPAPKEESRER"
     gene            8511..9125
                     /gene="pth"
                     /locus_tag="VGB88_07860"
     CDS             8511..9125
                     /gene="pth"
                     /locus_tag="VGB88_07860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9647398.1"
                     /translation="MLLFVGLGNPGPSYAWNRHNVGFMALDGMVQRYRFAAYRARFHG
                     LLADGRIDGERVIALKPTTYMNRSGIAVSQAAQFYKLAGADVVVIHDEIDLAPGKVRV
                     KTGGSSAGHNGLKSIDGHFGKDYRRVRIGIGHPGDPRRVESYVLQDFAKDDLAWLDPV
                     LDAVVDSAPYLVRGDDAGFMNRVAMLTRPPARERPARGDGGDGV"
     gene            9115..10215
                     /gene="ychF"
                     /locus_tag="VGB88_07865"
     CDS             9115..10215
                     /gene="ychF"
                     /locus_tag="VGB88_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9647399.1"
                     /translation="MGFRCGIVGLPNVGKSTLFNALSGTAAAAAENYPFCTIEPNVGR
                     VPVPDARLERIAALAKAPRTVPTWLDFVDIAGLVAGASRGEGLGNQFLGHIREVDAIA
                     HVLRCFEGEGVAHVAGRVDPCADAEIVDTELMLADLDSLERRLDGLVKRARGGDAEAK
                     AMLALAERAAAALRAGQPGRGLEVAREQRRWWRELQLLTAKPVLYVCNVDEASAATGN
                     RHSRAVEAMAQAAGAEAVVISVAIEADIAQLADAAERRAFLDSLGLAEPGLARLIRAG
                     HRLLGLITFFTAGPKEARAWTVPAGTTAQAAAGGIHTDFARGFICAETISCADYLGAG
                     GEQAAKDAGLMRQEGRDYVVRDGDVIRFRFNV"
     gene            10307..10993
                     /locus_tag="VGB88_07870"
     CDS             10307..10993
                     /locus_tag="VGB88_07870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9647400.1"
                     /translation="MSEIKGKFVELNAADDHRYQAYHVEAGGEHGRRGGLVVVQEIFG
                     VNSHVRSVADGFAAQGYEVYAPAVFDRVERHVELGYTDQDIERGFALKEKCDMTGVLL
                     DVQAPIATMRPEGTVGIVGYCWGGLISWMAACRTIGLSGAVCYYGGGIAQNLAHKPRC
                     PVLMHFGSRDKHISAADVDAVRAAQPGVEILVYDADHGFNCDQRGSYDAPSAKQALER
                     TLAFFRAHVG"
     gene            11027..11410
                     /locus_tag="VGB88_07875"
     CDS             11027..11410
                     /locus_tag="VGB88_07875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9647401.1"
                     /translation="MSRAGGLCLGLVLAASLALAAELGIEELVVGTGPTAQAGDTVEV
                     NYTGWLEATGEKFDSSLDRNQKFVFPLGVGQVIRGWDEGLVGMRVGGKRRLLIPPEYG
                     YGDRNVGPIPANSTMIFEVELLAIR"
     gene            11418..12305
                     /locus_tag="VGB88_07880"
     CDS             11418..12305
                     /locus_tag="VGB88_07880"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX9647402.1"
                     /translation="MSAGGDAIGIIGGSGVYRLDGLANTRWQGIDSPFGEPSDELLFG
                     ELDGQRLVFLPRHGRSHRIPPSEINFRANIDALKRAGCRELISVSAVGSLKEVLSPGT
                     FVLIDQFIDRTFARAKSFFGTGLVGHVSMAHPVCGRIGEHLMRAAAEAGIAMVRGGTY
                     LVMEGPQFSALAESELYRSWGCDVIGMTNMPEAKLAREAEMCYATVAMVTDYDCWHPH
                     HDHVTVEAIIGVLTANADRARALITASVPLLRDDRGAACPRGCHAALETALITAPEAR
                     DHELAAKLDAVAGRVLGRG"
     gene            12392..13330
                     /locus_tag="VGB88_07885"
     CDS             12392..13330
                     /locus_tag="VGB88_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class oxidoreductase"
                     /protein_id="HEX9647403.1"
                     /translation="MFRPGRLTLGLIFPIESYGLVHWDMPTMAGQDQMAKTAEALGFA
                     ALWVRDVPLRDPNFGDVGQIYDPWTWLGVVTANTRDIALATGSIILPLRHPLHVAKAA
                     ASIDHLSGGRLVMGVGSGDRPIEFPAFAVPYEERGPRLAESMAFIRRAWASEYPFIRS
                     PLGTLQHADLVPKPHAAGIAMLVTGHSQQSLEWIAANSDGWLTYARHPAHQKPLADRW
                     RALTGDAAPGLFKPFAQGLHVDLAEDADEDPRLIHNGYRLGRKRLIELFEVQQHIGVN
                     HCIINLKFARRPAAEAVQEIAEEVLPLFPTPAEAGT"
BASE COUNT         1901 a         5119 c         5081 g         2010 t
ORIGIN      
        1 tcggacgtgg cgaccacgct cgaggggctc ggccgcgagc tcggcatctc gaaggaacgg
       61 gtgcgccaga tcgaggcgcg ggcgatggcc aagctgcgcc gcatcctcag ccagcgcgtc
      121 ggcgagcagg cgacggtctg aggccgccgc cgcctcagcc cgacgccagc tcgcggctgc
      181 gctcggtggc ggcggcgacc gcgcgcgtca tcagcgcctc gagcccgtcc gcggccatca
      241 gcacgtcgag cgcggcggcg gtggtgccgc cggggctggt gacgttgcgg cgcagcgtct
      301 cggcgctgtc ctccgagcgc caggcgagct cgccggcgcc ggtgacggtg gcgcgcgcga
      361 gccgggcggc aagatcgggc gctaacccga ccgcgacccc ggcggcggcc aggcactcga
      421 tcatcaggaa cacgtaggcc gggccgctgc ccgacaggcc ggtgaccgcg tccatgaggg
      481 cctcgtcgcc gatccaggcg acctcgccga ccgcttcaag cagctccgtg cacagcgcga
      541 tctgctcggc gccgacgcgc gcattggcga ccgcgacggt gatgccgcgg ccgaccgcgg
      601 ccggggtatt gggcatcgcc cgcaccaccg cggcctgcgc ccccagccgc tgctcgaaca
      661 gcgcgagccg ggtgccggcg gcgatcgaca gatataccgg cgccgggccc ttggcgcccg
      721 cggggcggcg atagtccgcg accacgcccg cgatcacctg cggcttgacc gcgaacacca
      781 ccaccgccgg cgcctgcccg ccgtcgagcg cggccggcga gtcgatgacc gtcacgccgt
      841 ggcggccgag cgcacgcgcg gcgtccgcgg cgggctcgac caccaccacc tcgccggccg
      901 cgatgccgcc cttgcccagc cagccggcga gcagcgcgcc gcccatcttg ccgccgccga
      961 ccaggacgac gcgcgccgcc accgctcagg cttcgccgac cgggtcgacc aacgccgccg
     1021 cgatcgcctg gtcggcgtcc ttgccggccc agatcacgta ctggaacgcc gggtagaagc
     1081 ggtcgcactc gctgcgggcg gcgtcgatca ggtcatcgag caccgccgcg ctgatcttcc
     1141 cctcgctgcc ggcgatcacc gtgtgccgga aggtcggcca gccctcgtcc gcccacagct
     1201 cgaaatggcc cacccatagc cgctggttga tcttggccag caacggatag accgcttggc
     1261 gccgttccgc cggcacgcgc atgtcgagcg cgcaggtgaa gatcatcgag cgcgtctcct
     1321 cgcggcgggc gaaccacatg tggatgtcgc accattggcc cggcatctgg accgcgagct
     1381 cgttctcgcc ctggcgctcg aaattccagt cgttggccgc gaccagctcc tcgaccaggt
     1441 cgatcggatt gccggcgggc tcgagctcga ggaaggctat cctggccaac gcggcgtcct
     1501 agccagcggc ccgcatgagc cgcgggcgac gcacccgcgc agcaaaggcc gcgaacgacc
     1561 tacgccccat cttgtggtgt tcgaaggccg atcccacaat ggacggtaga ctgccagaac
     1621 cgcccaaggt gcgcaagcgg catgcgcggc gccgggcggg gaccctaagc cttattgtcc
     1681 tcggccttgc ccgcgtcctt ggccgcgccc ttgcccgcgg cacgccggcg ggcgccggct
     1741 tcgagcaccg ccaggcgttg ctcgagcgcg gcgagccgct cggcgagcac ctcggcctgc
     1801 tcgcgcgccg tcgccgccag cgcctgcacc gcctcgaact cgtcgcgccg gaccaggtcc
     1861 atgttcgcgg ccaggcgctc ggcgtgcgcg cgcaagcgcg cctccgcctc ctcgcgcagc
     1921 gccgccagcg aggacatcgc gccgcccgcc atgcgcgaca ggtcgtcgag cagtttcgag
     1981 tctctttcca tgccggtcac ctccgagttg cgcgcctata tcaggtaatc agcggggcgc
     2041 gtgcccgcaa cgccacgtcc ttgtcgcccc atgcccggac ccctataacg cgcaccatga
     2101 tcgtggtcac cggcggcgcc ggcttcatcg gctcgaacct ggtcgccgcg ctcgccgagc
     2161 gcgagatctt cgaggtggtg gtgtgcgaca ccttcggcga cggtgacaag tggcgcaacc
     2221 tcgcccgcca cgaggtcgcc gacatcgtgg cgcccgagca cctgttccgc ttcctgatca
     2281 atcgcgcccg gcgcggcgac ggctggggcc gggtcgaggt ggtggtccat ctcggcgcca
     2341 actcctcgac caccgagacc gacgtcgatg cgatgctgcg ccagaacttc ggcttctcga
     2401 tgaccttgtg gcggctgtgc accgagcacc gggtgcgcct gatctacgcc tcgtcggcgg
     2461 cgacctacgg cgacggcggc gccggcttcg acgacgatgc gcgcgccgac gccctcgccc
     2521 gcctgcgccc gctcaaccct tatggctgga gcaagcacct gttcgaccgc cgcgcggtgc
     2581 gcctggcggc gagcgcggac acgcggccgc cgcaatgggt cgggctcaag tttttcaacg
     2641 tctacggccc gaacgagtac cacaagggcg cgatgcagag cgtggtggcg cagaagtttc
     2701 cgctcgcccg cgccggcaag ccggtgaccc tgttccgctc gtgcgatcca gactacgccg
     2761 acggcggcca gatgcgcgac ttcgtctatg tgcgcgactg cgtcgaggtg atgatgtggc
     2821 tgctcgacca cgccgaggtc tgcggcctgt tcaatgtcgg caccgggcgg gcgcgcagct
     2881 tcctcgacct cgcccgcgcg ctccagcgcg ccctcggcgc cgacgagcgc atcgagttca
     2941 tcgacatgcc ccaatccctg cgcgcgcagt accagtactt caccgaggcg cgcatcgacc
     3001 ggctcaggcg cgccggctac gatgccccgt tcaccaccct cgaggccggg gttgccgact
     3061 acgtggccaa ctacctggcg accgacgacc cataccggtg agcggcgtgc tggcgttcgc
     3121 cgcgcttagc tttcccgaca tcgacccggt ggcgatccag ctcgggccgg tggcgatccg
     3181 ctggtacgcg ctcgcctaca tcgccggcct ggcgctgggc tggggctatg tgcgccggct
     3241 ggcgcagacc ggcgtcgccg cgttcaccaa ggccgaggtc gacgacctct tgttctgggt
     3301 cgcgctggga gtgatcctgg gcgggcggct gggctacgcg ctgttctacc gggccgacca
     3361 ctacctctcg aatccattgg agctggtcat gctatggcgc ggcggcatgt cgttccacgg
     3421 cgggcttctg ggcgtggtgg tggcgctggc ctggttcgcc cgccaccgcc gccggccgct
     3481 gctcgcggtc gccgacgcgg tcgcggtggc gatcccgatc gggctcctgt tcgggcgcct
     3541 ggccaacttc atcaacggcg agctgtatgg acggattgcc gatgccgact tgccgtggtc
     3601 gatgatcttc cccaccggcg gcccgttccc gcgccatccg agccagctct acgaggccgg
     3661 gctcgagggc ctggcgctgt tcgccgtgct tgcctggctg gtgcaccggc agggcgcgct
     3721 cgaccggcgc gggctggtta ccggcacctt cctggtcggc tacagcatcg cgcgcgcagc
     3781 cggcgagctg ttccgcgagc ccgacgccca tatcggcttc ctgatcgggg gcatgaccat
     3841 gggccagttc ctgtcgatcc cgatgctgct gttcggcgcc tggctgatcc tgcgcgcgcg
     3901 cccggcggcg cgcaacgcgg gatgaccgcg ctcggcgcgc gcatccgccg gcgcatccgc
     3961 cttggcgggc cgatcacgct cgcccaatac atggccgagg cgctcgccaa cccggccgac
     4021 ggctattacg gcggcgaggc cggcggggcg gtgatcggcg cgcgcggcga tttcgtcacc
     4081 gcgcccgaga tcagccagat gttcggcgag ctgatcggcc tgtggtgcgc cgagatgtgg
     4141 cgccggatgg gtgcgcccga cccggtgcgg ctggtcgagc tcggccccgg gcgcggcacg
     4201 ctgatggcag atgcgctgcg cgccgcggcg acggtgccgg cgttgcgcgc cgcgctccgc
     4261 atccatctgg tcgagaccag cccgcggctg cgcgcgcgcc aggccgagac gctcgccggc
     4321 agcggcgccg actggcacga cagcctggcc gatgcgcccg acgggccgct gctgctggtc
     4381 gccaacgagt ttttcgacgc cctgccgatc catcagttca ccgccaccgc cgcgggctgg
     4441 cgcgagcgcc tggtcggcgt cgccggcgac ggccgcctcg cgctctgcgc ggccccgggc
     4501 gcgaccccgg cgctggcgct gctcgaccgc ttgggcgtcg agccggtgcc gggcgcggtc
     4561 gccgagctgt gcccggccgg gatcgcgctc gccgccacca tcggcgcgcg cgtcgcggcc
     4621 gagcgcgggg cggcgctgat catcgactac ggcgccggcg gcgcgctcgg cggcagcgtc
     4681 caggcggtgc gcgcgcatcg cgtcgtcgcg ccattggccg agccgggcgc ggccgacctc
     4741 agtgcccatg tcgacttcgc cgcgctggcg cgcgcggcgc gcgaggccgg ggccgcggtg
     4801 catgggccgg tggcccaggg cgcgttcctg cgcgcgctgg ggctcgaggc ccgtgccgcg
     4861 cggctcgcgc gccgggcgac tcccgcgcag caggccgcgc tcgcggcggc ttgcgcgcgc
     4921 ttggccggcg atgaccagat gggcgcgctg tacaaggtgc tggcgatcgc gcatccggcg
     4981 ttgccgacgc cagcggggtt cggcgaggaa tgagcgcgcg ggcgctgacc gatgccggcc
     5041 tcgccgccgc cccgggcgtc gcgcacggat tcttcacccg ccggggcggc gtgtcggcgg
     5101 gggtgtacgg ctcgctcaac tgcggcctcg gctcgggcga cgccggcgcg gcggtggccg
     5161 agaaccgggc ccgcgccatg gccgcattgc gcctcgggcc agaggcgctg gtcaccggct
     5221 accaggtcca cggcatcgag gtgcgcatta tcgacggcaa cgaaacggct ggcgaaaggc
     5281 gcgcgcggcg ccacaccgac gccgccgtca cgagcgcgcc cggcatcgcg ctcggcgtgc
     5341 tgaccgcgga ctgcgcgccg gtgctgctgg ccgacccgga ggcgcgcgtg atcggcgccg
     5401 cccacgccgg ctggcgcggc gcgctggccg gggtggtcga ggcaacggtg gcggcaatgg
     5461 agcgcctggg cgccagccgc ggcgctatcg ttgccgcgat cggcccgtgc atcgccgccg
     5521 agtcctacga ggtcgggccc gaattcccgg ccccgttcct ggccgacgac gcggcgagcg
     5581 cgcgcttctt cgcgccgcgc cggtcgggcg ggcggctgtc gttcgacctt gccggctacc
     5641 tcgcctggcg gctcggccgg ctcgggctcg gcgaggtcgg cgcgaccggc ggcgatacct
     5701 gtgccgatga cgccgccttc ttcagctacc ggcgggcgta ccgccgcggc gagaccgatt
     5761 acggccggct gctgtcggcg atcgcgctcg agtcctgacg cggggccgcg atgccgtacc
     5821 tggtgtggtt cgtgaccctg ctgatcgtcg ccctggtggc gagctgcgtg ctggccgcgt
     5881 gccagccggt gccgcggccg ttccagcccg agaccaagtc cgccgccgac ttcgccggct
     5941 tcaccggcgt cgccgacaag ctggggatct tcgtcgccgg tatcgacaac gcgccggccg
     6001 ggctcgaccg ggcggtggtg ggcgcgctgg tcgagggcct gcacagccgc gacatcgccg
     6061 ccagcgaccg ggtcgccaac cgggcgagct acctgctgat cggcaccgcg cgccccgatg
     6121 cgcccgacgg gctggtcatc gcctggcggc tgatcgattc cgacggcctg gtgatcggcc
     6181 tggtcgacca gcacctgggc gtcgacgatg ccgcctggag gcgcggcgat ccggcgctgg
     6241 cggcggcaat cgcggaccag gcggcgccgc ggctggcggc ggtgatcgcc gatgccgccc
     6301 cggccgggcc ggcgccgcgg ctgccatcgc cgggcgcggt gatcctggcc gaggtcgagg
     6361 gcgcgcgcgg cgacggcgat gccgagctca cccgcgccat gcgcgcggcg ctcgagcgcc
     6421 tcggcatcgc ggtcgtggac acccccggcg ccgcgcaccc gatcgtccgc ggccaggtgc
     6481 tgcgccggcc ccgcggcgcc gacgagctgg tcgagatccg ctggtcggtg gaggcgccgg
     6541 acggcggcga gctcggccgg atcggccaga gcaacctggt gcccgcgggc agcctcgacg
     6601 gcgactgggg gccggtcgcg cgggcggtcg cggacggcgc cgcggagggc gtgcgggcgc
     6661 tgctcgacga gctcgcgcac gcgcccaaat aggcgcgccg gccgccggtg tgcggcgttt
     6721 acaccttggg gccggcttgt tacagtccgc cgcccttcgc gggccagtca gcttggagat
     6781 gcgggcgccg ggtcgggcgc gcggccagga cgtcatgaag atcgtcgccg ggaacagcaa
     6841 ccggccgctg gccgaggcgg tcgcggcgtt ctgtaacttg ccgctggtca atgccagcat
     6901 ccgccggttc tcggaccagg aagtgttcgt ggagatccac gagaacgtcc gcggcgagga
     6961 catgttcctg gtccagtcga cctcgtaccc ggccaacgac aacctgatgg agctgctggt
     7021 cacgctcgat gcgctgcgcc gcgcctcggc gcgccggatc accgccgtgg tgccctattt
     7081 cggctatgcc cgccaggaca ggaagcccgg gccgaggacg ccgatctcgg ccaagctggt
     7141 ggccaacctg atcaccgtcg cgggcgccga ccgggtgctg accatggacc tccatgccgg
     7201 ccagatccag ggcttcttcg acatcccggt cgacaacctc tacgccgcgc cggtgatgat
     7261 cgccgacatc aagcagaact tctccggcga caagctggtg gtggtctcgc ccgacgtcgg
     7321 cggcgtggtg cgcgcgcggg cgctggccaa gcggattgac gccgatctcg ccatcgtcga
     7381 taagaggcgc gagcgtgccg gcgtctccga ggtggtcaac atcatcggcg aggtcgacgg
     7441 gcggcgctgt cttctggtcg acgacatcgt cgattccgcg ggcaccctgt gcaatgcggc
     7501 agtcgcgctc aaggacaacg gcgcgaccac ggtgatggcc tacgtcaccc atggcgtgct
     7561 gtcgggcggg gcggtggcgc gggtcgcctc ctctgagctc gaggagctgg tcatcaccga
     7621 caccatccag gcgaccgagg cggtgcgggt ggcgaacaac gtccggatgc tgtcgatctc
     7681 gcagctgatg gccgaagcga tgcgccggat aagcgaggag agctcggtct cgagcctgtt
     7741 cgactgacac gtgagcggaa agttctaagg agcaagtaga atcatggccg acagtctgat
     7801 catcagcgcc gagccgcgcg cggcggcggg caagggcgcg gcgcgggcga cgcgccgggc
     7861 cgggcgcgtg ccggcggtgg tctacggccg cggcgagcag ccgatcgcgg tctcggtcga
     7921 ccgccacgag cttgcccagg agtaccgccg gggcggcttc ttcagccgcc tctacgacct
     7981 caagctcggc gaggagaacg tccgcgtgct gccgcgcgag gtccagtcgg acccggtgac
     8041 cgacgcgccg ctgcacgtcg acttcctgcg ccttgcggcc gacagccgga tcgacgtcga
     8101 cgtgccggtg atgttcatca acgacctgga gtcgcccggc ctgcgccgtg gcggcgtgct
     8161 caacgtggtc cgccacgtgg tcgagctcaa ctgccgcgcg gactcgatcc cggaatcgat
     8221 cacggtcgac ctgaccggcc tgcagatcgg cgactcggtg cacatcagcg ccattgcgct
     8281 gcccgagaac gtgcgcacca ccatcgccgg gcgcgacttc actatcgcca ccgtcgccgc
     8341 gccgacggtg gtcgccgagg aggccgccgc cgaggcggcc gaggcggcgg agggcgaggc
     8401 ggcggtgccc gagggcgagg cggcggcgcc cgaggccggc gagccggcgc ccaaggagga
     8461 gtcgcgcgag cgttaggcga gggcgccttc gccgcccgcg cgcgggccct gtgctgctgt
     8521 tcgtcggcct cggcaatccg ggcccgagct atgcctggaa ccgccacaat gtcggcttca
     8581 tggcgctcga cggcatggtc cagcgctacc gcttcgccgc ctaccgggcg cgcttccacg
     8641 gcctgctcgc cgacggccgg atcgacggcg aacgggtgat cgcgctcaag cccaccacct
     8701 acatgaaccg gtcgggcatc gcggtctcgc aagcggcgca gttctacaag ctcgccggcg
     8761 cggacgtggt ggtgatccac gacgagatcg acctcgcccc gggcaaggta cgggtcaaga
     8821 ccggcggcag cagcgccggg cacaacggcc tgaagagcat cgacggccat ttcggcaagg
     8881 actaccggcg ggtgcgcatc ggcatcggcc atcccggcga tccgcgccgg gtcgaatcct
     8941 acgtgcttca ggacttcgcc aaggacgacc tcgcctggct cgacccggtg ctcgacgcgg
     9001 tcgtcgactc ggcgccctat ctggtgcgcg gcgacgatgc cgggttcatg aaccgggtgg
     9061 cgatgctgac ccggccgccg gcgcgcgagc gcccggcccg cggcgacggc ggcgatgggg
     9121 tttaggtgcg gcatcgtcgg cctgcccaac gtcggcaagt cgaccctgtt caacgcgctt
     9181 tccggcaccg cggcggcggc ggccgagaac tacccgttct gcaccatcga gcccaatgtc
     9241 gggcgggtgc cggtgccgga tgcgcggctc gagcgcatcg ccgcgctggc caaggcgccg
     9301 cggacggtgc cgacctggct cgacttcgtc gacatcgccg gcctggtcgc gggcgcaagc
     9361 cgcggcgagg ggctgggcaa ccagttcctc ggccacatcc gcgaggtcga cgccatcgcc
     9421 cacgtgctgc gctgcttcga gggcgagggc gtcgcccatg tcgccggccg cgtcgacccc
     9481 tgcgccgacg ccgagatcgt cgacaccgag ctgatgctcg ccgacctcga cagcctggag
     9541 cggcgcctgg acgggctggt caagcgcgcg cgcggcggcg atgcggaggc gaaggcgatg
     9601 ctggcgctgg ccgagcgcgc cgcggcggcc ttgcgcgccg gtcagcccgg gcgcggcctc
     9661 gaggtcgcgc gcgagcagcg ccgctggtgg cgcgagctgc agctgttgac cgccaagccg
     9721 gtgctgtacg tgtgcaacgt cgatgaggcg agcgccgcca ccggcaaccg acattcccgc
     9781 gcggtcgagg cgatggccca ggccgcgggc gccgaggcgg tggtgatctc ggtcgcgatc
     9841 gaggcggaca tcgcccagct cgccgacgcc gccgagcggc gcgcgttcct cgacagcctc
     9901 gggcttgccg agcccgggct cgcacgcctg atccgcgccg gccaccggct gctcggcctg
     9961 atcaccttct tcaccgccgg gcccaaggag gcgcgcgcct ggacggtgcc ggcggggacc
    10021 accgcccagg cggcggcggg cgggatccac accgacttcg cgcgcggctt catctgcgcc
    10081 gagaccatct cgtgcgccga ctacctcggc gccggcggcg agcaggcggc gaaggacgcc
    10141 gggctgatgc gccaggaggg ccgcgactac gtggtccgcg acggcgacgt gatccgcttc
    10201 cggttcaacg tttgaggccg gcggcgcgat tgaacggcgg gcgcgaaacg gctaagacca
    10261 cggggccgtc gggccgccgg ggacaggcgc aggggagcag cgagagatga gtgagatcaa
    10321 gggcaagttc gtcgagctca atgccgccga cgaccaccgc taccaagcct accacgtcga
    10381 ggccggcggc gagcacggcc gccgcggcgg gctggtggtg gtgcaggaga tcttcggcgt
    10441 caacagccac gtccgcagcg tcgccgacgg cttcgccgcc cagggctacg aggtctacgc
    10501 gccggcggtg ttcgaccgcg tcgagcgcca cgtcgagctc ggctacaccg accaggacat
    10561 cgagcgcggc ttcgcgctca aggaaaagtg cgacatgacc ggggtgctgc tcgacgtcca
    10621 ggcgccgatc gcgacgatgc gtcccgaggg cactgtcggc atcgtcggct actgctgggg
    10681 cggcctgatc agctggatgg ccgcgtgccg aacgatcggg ctgtcgggcg cggtgtgcta
    10741 ttacggcggc ggcatcgccc agaacctcgc ccacaagccg cgctgcccgg tgctgatgca
    10801 tttcggcagc cgcgacaagc atatcagcgc ggccgacgtg gacgcggtgc gcgcggcgca
    10861 gcccggggtc gagattctcg tctacgacgc cgaccacggc ttcaactgcg accagcgcgg
    10921 ctcctacgac gcgccgtcgg cgaagcaggc gctcgagcgc acgctcgcct ttttccgcgc
    10981 ccatgtcggg tgatcgaggc cggggaggat cgacatgctg caattgatga gccgcgcggg
    11041 cgggctgtgt ctcgggctgg tgctggcggc gtcgctggcg ctcgccgccg agctcggcat
    11101 cgaggaactg gtggtcggca ccggcccaac cgcccaggcc ggcgatacgg tcgaggtcaa
    11161 ctacaccggc tggctggagg cgaccggcga gaagttcgac agctcgctcg accgcaacca
    11221 gaagttcgtc ttcccgctcg gcgtcggcca ggtgatccgc ggctgggacg agggcctggt
    11281 cggcatgcgg gtcggcggca agcggcggct gctgataccg cccgaatacg gctacggcga
    11341 ccgcaacgtc ggcccgatcc cggccaactc gaccatgatc ttcgaggtcg agttgctcgc
    11401 catccgttag cccgctcatg tccgccggcg gcgacgcgat cgggatcatc ggcggcagcg
    11461 gcgtctatcg gctcgacggg ctcgccaaca cccgctggca gggaatcgac tcgccgttcg
    11521 gcgagccgtc ggacgagctc ctgttcggcg agctcgacgg tcagcggctg gtgttcctgc
    11581 cccgccacgg ccgcagccac cggattccgc cgagcgagat caacttccgc gccaacatcg
    11641 acgcgctcaa gcgcgccggc tgccgcgagc tgatctcggt cagcgccgtc ggctcgctca
    11701 aggaggtgct gtcgcccggc accttcgtgc tcatcgatca gttcatcgac cgcaccttcg
    11761 cgcgcgccaa gagcttcttc ggcaccggcc tggtcgggca cgtgtcgatg gcgcacccgg
    11821 tgtgcgggcg gatcggcgag cacctgatgc gcgcggcggc cgaggccggg atcgccatgg
    11881 tgcgcggcgg cacctatctg gtgatggagg ggccgcagtt ctccgcgctc gccgaatccg
    11941 agctttaccg cagctggggc tgcgacgtga tcggcatgac caacatgccc gaggccaagc
    12001 tcgcgcgcga ggcggagatg tgctacgcga cggtcgccat ggttaccgac tacgactgct
    12061 ggcatccgca tcacgaccac gtcacggtcg aggcgatcat cggcgtgctg acggccaatg
    12121 ccgaccgggc gcgggcgctg atcacggcgt ccgtgccgct gctccgcgac gaccgcgggg
    12181 ctgcgtgccc gcgcggctgc cacgccgcgc tcgagacggc gctgatcacc gcgcccgagg
    12241 cgcgcgacca cgagctcgcc gccaagctcg acgcggtcgc gggacgcgtc ctcgggcgcg
    12301 ggtgagcgag cacgcgacac cccgcgcggg cgcggcggag cctgcggagg cggggccgcg
    12361 cgagccgtcg ttcaaccgcg gcttccggcg gatgttccgc cccggccggc tcaccctcgg
    12421 cctgatcttc ccgatcgaat cctacggcct ggtccattgg gacatgccga ccatggccgg
    12481 ccaggaccag atggcgaaga ccgccgaggc gctcggcttc gccgcgctgt gggtgcgcga
    12541 cgtgccgttg cgcgatccca acttcggcga cgtcggccag atctacgacc cgtggacgtg
    12601 gctcggcgtg gtcaccgcca acacccgcga catcgcgctt gccaccggca gcatcatcct
    12661 gccgctgcgc catcccctcc acgtcgccaa ggccgcggcc tcgatcgacc acttgagcgg
    12721 cggccggctg gtgatggggg tcggctcggg cgaccggccg atcgagttcc cggcgttcgc
    12781 cgtgccctat gaggagcgcg ggccgaggct cgccgagtcg atggcgttca tccgccgcgc
    12841 gtgggcgagc gagtatccgt tcatccgctc gcccctcggc accctccagc acgccgacct
    12901 ggtgcccaag ccgcacgccg cgggcatcgc catgctggtc accggccaca gccagcagag
    12961 cctggagtgg atcgccgcca acagcgacgg ctggctcacc tacgcccgcc acccggcgca
    13021 ccagaaaccg ctcgcggacc gctggcgcgc gctcaccggc gacgccgcgc ccgggctgtt
    13081 caagccgttc gcgcaaggcc tccacgtcga cctcgccgag gatgcggacg aggacccgcg
    13141 cctgatccac aacggctatc gcctggggcg caagcgcctg atcgagctgt tcgaggtcca
    13201 gcagcacatc ggcgtcaacc actgcatcat caatctcaag ttcgcccgcc gcccggccgc
    13261 cgaggcggtc caggagatcg ccgaggaggt gctgccgctg ttcccgacgc ctgccgaagc
    13321 gggtacttga acgtgcacat gaccctgcgg catatgccgt tcggaacaaa acttttacgt
    13381 ttaagcaaac gcgtttcccc gtcataccga tgccgtatga tcgattcggg atagcagccg
    13441 tgtcttcatt tcgtcatcgc cgtccccgga tttaacccgg cggtgatccg gcgatccatg
    13501 gatgcgcgga tcaagtccgc gcatgacgat acgggaacga atcataccag cgagccaacc
    13561 aattgactcg ccggcgcccg tcgacaccaa tgcgtcaccc cgggcgaggc ggagccgaga
    13621 cccggggtcc aggacggcgc gaagcgccgt cggcgcctgg gggtccggcc accggattac
    13681 gcggccctgg attccggctc tccgctccgc tccggccgga atgacggcat gggcggcgtc
    13741 gttcatatgc tcgctggtat gactcgaaat gaattgtacg tagttgatac gttacggaac
    13801 cgtcatgcgt gggcttgacc cgcgcatcca cgtcgacccg cttggaattt cgccgatgga
    13861 aggacgtgca tggccgtgtc aagcacggcc atgacgatga agtaagaggg cgtcgcgact
    13921 cccgcaccct ccgcgcctcc gcgtgcgcga agcagtctcg gcggcgctat gcgctgcgcg
    13981 ggcgggtgag cgcggcgagc gctgcggggg tgtcgatgtc ggcaagcgtc gcgtcgtcgg
    14041 ccatctcgac ctcgcacacc gcgtccgcgt tctcgccgat caggtggcgg gcgccgacgt
    14101 cgcccgcgac c
//
LOCUS       DASWHU010000214         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2341444, whole genome shotgun sequence.
ACCESSION   DASWHU010000214 DASWHU010000000
VERSION     DASWHU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341444"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..903)
                     /locus_tag="VGB88_07890"
     CDS             complement(58..903)
                     /locus_tag="VGB88_07890"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9647404.1"
                     /translation="MTRPRSPIPAAALVLWALAAAGARAGEDGGAALTLDYRVYLGGL
                     HVLSLSSALDQEREGGYRMTVAAHTDGLIGKFVDASYEAESDGRAEGAVARSERYRGR
                     SRDGDDAARSVAVTYRRDAAPEVVFEPADAAPDEPLPADTIAATTDPVSAMLTLMRTL
                     AETGRCEATMRVFDGRRRYDLVASDAGERTLTASRLAPYGGPARECSVRIDRIAGFRD
                     GKLAKRYPDEIAIYLAPVFPGAPPVPVRLHANNLFGALRMHLVNPQPAAADERAGLDG
                     ALSRE"
     gene            complement(934..1413)
                     /locus_tag="VGB88_07895"
     CDS             complement(934..1413)
                     /locus_tag="VGB88_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9647405.1"
                     /translation="MAHGKVGCGRRGTVRAIAAAAVLALPAAALGQAENDGTLSIDPE
                     LQRPSTLAVTLPNMDSTRGRILFQTKGCVICHEVNGVGGRRGKAPSLDAARMSIEPNP
                     FEFSARMWRGAEDMIALQKEDLGYKLEIRADELADIFAFVHDQDEQKLFTLPGRTTQ"
     gene            complement(1470..>2015)
                     /locus_tag="VGB88_07900"
     CDS             complement(1470..>2015)
                     /locus_tag="VGB88_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9647406.1"
                     /translation="RVAPGQVVLVQGTGGVSLFALLFARMLGARVIATSSRADKLERA
                     RALGADHVINYRDDPEWGRSARELAGGAGVDLVVEVGGAGTLEQSLRAVRVAGTIAMI
                     GVLSGAEARLSLGQVVTRSVTLQGVTIGNRDMLEELIRAIDHNGLEPPIDDRVYGFEE
                     LVEALRAFPEGAHFGKVCIRF"
BASE COUNT          263 a          733 c          740 g          279 t
ORIGIN      
        1 cgctcaacag cgaaaacacg atcgcggacg tgatcggcgc ctaccggcgg ctgcggctca
       61 ttcgcggctg agcgcgccgt cgagcccggc gcgctcgtcg gcggcggcgg gctgcgggtt
      121 gaccaggtgc atgcgcagcg ctccgaacag gttgttcgcg tgcaggcgca ccggcaccgg
      181 cggcgcgccg ggaaacaccg gcgccagata gatcgcgatc tcgtcgggat agcgcttggc
      241 gagcttgccg tcgcggaagc cggcgatgcg atcgatgcgc acgctgcact cccttgccgg
      301 gccaccatag ggcgccaggc gcgaggcggt gagcgtgcgc tcgccggcgt cgctcgcgac
      361 caggtcgtag cggcgcctgc cgtcgaacac gcgcatggtc gcctcgcagc ggccggtctc
      421 ggcgagagtg cgcatcaagg tcagcatggc gctcaccgga tcggtggtcg cggcgatggt
      481 gtccgcgggc agcggctcgt ccggcgcggc gtcggcgggc tcgaacacca cctcgggcgc
      541 ggcgtcgcgg cgataggtca cggcgacgct gcgggccgcg tcgtcgccgt cgcgcgagcg
      601 gccgcggtag cgctcggagc gggcgacggc gccctccgcc cggccgtcgc tctcggcctc
      661 gtaggaggcg tcgacgaact tgccgatcag gccgtcggtg tgcgcggcga cggtcatgcg
      721 gtagccgcct tcgcgctcct ggtcgagcgc gctcgacagc gacagcacgt gcaggccgcc
      781 gagatagacc cggtagtcga gcgtgagcgc cgcgccgccg tcctcgccgg cacgcgcgcc
      841 cgccgccgcc agggcccaca gcaccagggc ggcggccggg atcggggatc gtggtctggt
      901 catcgctcgc gaacggcctg cggcggccgc tccctactgg gtggtgcggc cgggcagggt
      961 gaacagcttc tgctcgtcct ggtcgtggac gaaggcgaag atgtcggcca gctcgtcggc
     1021 gcggatctcg agcttgtagc cgaggtcctc cttctgcagc gcgatcatgt cttcggcgcc
     1081 gcgccacatg cgcgcggaga actcgaacgg gttgggctcg atcgacatgc gtgccgcgtc
     1141 caggctcggc gccttgccgc ggcgcccgcc gacgccgttg acctcgtggc agatcacgca
     1201 gcccttggtc tggaacagga tccggccgcg ggtcgagtcc atgttgggga gcgtcaccgc
     1261 cagggtggag gggcgctgga gctcggggtc gatcgacagg gtgccgtcgt tctcggcctg
     1321 gccgagcgcg gccgccggca gcgccagtac cgcggcggcg gcgatcgcac gcaccgtccc
     1381 gcgccggccg catcccacct tgccatgcgc catcgcccgg tcctcctccg attgcggccg
     1441 cagccgcggc cgggtgcgat attacgccgt tagaagcgaa tgcacacctt gccgaaatgc
     1501 gcgccctcgg ggaatgccct gagcgcctcc accagctcct cgaagccgta gacccggtcg
     1561 tcgatcggcg gctccaggcc gttgtggtcg atggcgcgga tcagctcctc gagcatgtcg
     1621 cggttgccga tcgtgacccc ctgaagggtg acgctgcggg tcaccacctg gccgaggctg
     1681 agcctggcct cggcccccga cagcaccccg atcatggcga tggtgccggc cacccgcacc
     1741 gcgcgcagcg actgctcgag cgtgcccgcg ccgccgacct cgaccaccag gtcgacgccg
     1801 gccccgccgg ccagctcgcg cgcgctccgc ccccactcgg gatcgtcgcg gtaattgatc
     1861 acgtggtcgg cgcccagcgc gcgggcgcgc tcgagcttgt cggcgcgcga cgaggtcgcg
     1921 atcacccgcg cgccaagcat cctggcgaac agcagcgcga acagcgacac cccgccggtg
     1981 ccctggacca ggaccacctg gccgggggcg acgcg
//
LOCUS       DASWHU010000215         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2341824, whole genome shotgun sequence.
ACCESSION   DASWHU010000215 DASWHU010000000
VERSION     DASWHU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341824"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VGB88_07905"
     CDS             complement(<1..1517)
                     /locus_tag="VGB88_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647407.1"
                     /translation="MTGALARPVLLAAVLGVACGPAWGADGADRIAAALAARGVSASA
                     LGLIGNAVAHRALPEPPAAGKGTQRYLDDPVAALAVADALDLPAAASPPPTLDWAPPA
                     DLPEPVRDFVARLVAAAADLTVHVAAMVPGPCCERAALIAAAAAYGGDAAVERLVREV
                     DRAALDRAAAAAMGVRALVLGAFAGVAHDAAGALLSGHELADALALIPGRRFSSPVGT
                     VVIAPAASTVHEAPAAIVIDVGGSDSYRLDRLGAEGTLVIVDLAGNDTYAGDDTAVRA
                     ARVSIDFAGDDLRESDGGGQGAAIGGVSVLHDGAGADRYRARVFAQGAAVAGFAALID
                     EAGDDRYQIGARGQGFGGPVGFGALVDRDGKDSYRAEGGLADPFGEPGGGLSFAQGAA
                     AGLGGRLAGGIGLLRDLAGDDRYEAEKYAQGVGADRGLGVLDERGGDDSLVATHYGQG
                     FGVRAGIGLVRERDGFDGYWLAEGAGQGAGLDFGIGALVDDAGGDRYQARRFAQGA"
     gene            1709..2797
                     /locus_tag="VGB88_07910"
     CDS             1709..2797
                     /locus_tag="VGB88_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEX9647408.1"
                     /translation="MDIAILGGGNGAYAAAADLSEQGHRVRLWRRDGAALAAVAESGR
                     IVLTDHQGRREVALHRVTADLGEAVDGAALVLAPVPATAQEDLARALAPHLRDGQVLF
                     LPPGTFGSYVMARAIADAGGAAIAIAEAGTLPWLARKRGPREVAISARATRLPAGVFP
                     AARAGAAFEVLQAALPAVEPLADALDGALVNAGPIIHPPLIVMNAGPIEHFERWDIHA
                     EGTQASIRRVTDALDEERVAVREALGYGAPHYRLRDHYAVSGEEWMYGNSAHERLVDS
                     GDWREPIDLAGHRYMREDVACGLAFLVSVARWAGVAAPVAGGLLAIASAMLGEDLRRT
                     GRTLEAMGLARLSRAEMRALLADGPGGA"
     gene            2794..3780
                     /locus_tag="VGB88_07915"
     CDS             2794..3780
                     /locus_tag="VGB88_07915"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9647409.1"
                     /translation="MSDRPAVAIVGAGRMGRGLAHVFAYAGHRVAVIDLKARPKDERR
                     RLADDARAEVRRSLEALADHGALPMARVEPLLSRVTIAGAAEAGEALAAAGFVFEAVP
                     ERLDAKRAALGVIGRAAAADAVVASATSTMRVDELAALIERPERFVNAHWLNPAYLVP
                     LVEVSPGRATSPDTLARLLALLEAVGKVAVVCAASPGFIVPRLQALAMNEAARLVEEG
                     VASPEDIDTATRFGLGFRFAAMGVVEFIDWGGGDILYYADEYLRQSLGAERFAAPDIV
                     RRNMEAGAVGVRAGRGFHDWQGRDLDAYQRETIARFVALLRHYGLLKPPAED"
     gene            3877..>4101
                     /locus_tag="VGB88_07920"
     CDS             3877..>4101
                     /locus_tag="VGB88_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647410.1"
                     /translation="MTGRTWPRLAAMLAMAVMPPAGALAAEDEATPPASYALETSDWS
                     EAAYDARPQIESYAMLVRASYAKAAADAAAL"
BASE COUNT          533 a         1539 c         1547 g          482 t
ORIGIN      
        1 gccccttgcg cgaatcggcg cgcctggtag cggtcgccgc ccgcgtcgtc gaccaatgcc
       61 ccgatgccga agtcgagccc ggcgccctgc cccgcccctt ccgccagcca gtagccgtcg
      121 aagccgtcgc gctcgcgcac caggccgatc ccggcgcgca cgccgaagcc ttggccgtaa
      181 tgggtggcga ccagcgaatc gtcgccgccg cgctcgtcga gcacgccgag gccgcggtcg
      241 gcgccgacgc cttgggcata tttctccgcc tcgtagcggt cgtcgccggc aagatcgcgc
      301 aacaggccga tgccgccggc gagccggccg ccgagcccgg ccgccgcgcc ctgggcgaag
      361 ctcagcccgc cgcccggctc gccgaacggg tcggccaagc cgccctcggc gcgatagctg
      421 tccttgccgt cgcggtcgac cagcgcgccg aagcccaccg gcccgccgaa gccctggccg
      481 cgcgcgccga tctgatagcg gtcgtcgccg gcctcgtcga tcagcgcggc gaagcccgcg
      541 accgccgcgc cctgggcgaa gacgcgcgcc cggtagcggt cggcaccggc accgtcgtgg
      601 agcaccgaca cgccgccgat cgccgcgccc tggccgccgc cgtcgctctc gcgcagatca
      661 tcgccggcga aatcgatcga cacgcgggcg gcgcgcaccg cggtgtcgtc gcccgcatag
      721 gtgtcgttgc cggcgaggtc gacgatcacc agcgtcccct cggcgccgag ccggtcgagc
      781 cggtagctgt cgctgccgcc gacgtcgatc acgatcgccg ccggcgcctc gtgcacggtg
      841 ctggcggcgg gcgcgatgac cacggtgccc accggcgagc tgaaccgccg gccggggatc
      901 agggcaaggg catcggccag ctcgtgcccg gaaagcagcg cgccggccgc gtcatgggcg
      961 accccggcga aggcgccgag caccagcgcg cgcacgccca tcgccgccgc cgccgcgcga
     1021 tcgagggcgg cgcgatcgac ctcgcgcacc aggcgctcga ccgccgcgtc accgccatag
     1081 gcggcggcgg cggcgatcag cgccgcgcgc tcgcaacacg gtccgggcac catggccgcc
     1141 acgtgcacgg tcaggtcggc cgcggccgcg accaggcggg cgacgaagtc gcgcaccggc
     1201 tcgggcagat cggcgggcgg cgcccagtcg agcgtcggcg gcggcgacgc cgcggcgggc
     1261 aggtcgaggg catcggcgac ggcgagcgcg gccacggggt cgtccagata gcgctgcgtg
     1321 cccttgcccg ctgccggcgg ctcgggcagc gcgcgatgcg cgaccgcgtt gccgatcagc
     1381 ccgagcgcgc tcgcgctcac gccgcgcgcg gccagcgccg ccgcgatccg gtccgcgccg
     1441 tccgcgcccc atgctgggcc gcacgccacg ccgagcaccg cggccagcag gacgggccgg
     1501 gcaagggcgc ccgtcacgcg cgcgagcaac ggaaacggga ggcggcggcg caaaggatgg
     1561 aacggaggtc ggtcatgggg gccccgcggt gtgccggcat tattggcagt ttgccgggcg
     1621 ctgaaaaggg cggcggcacg cttgacccgc ggacatcggc ccacagagga tgcggttacg
     1681 gaaagacagg gagcgggcgg gctcgggcat ggacatcgcg atactgggag gcggcaacgg
     1741 cgcctacgcc gccgccgcgg acctgtccga gcaaggccat cgggtgcgct tgtggcgccg
     1801 cgacggcgcg gcgctggcgg cggttgccga atccggacgg atcgtcctca cggatcacca
     1861 aggccggcgc gaggtggcgc tgcaccgggt caccgccgac ctcggcgagg cggtcgacgg
     1921 cgccgcgctg gtgctggcgc cggtgccggc gaccgcgcag gaagacctgg cccgcgcgct
     1981 cgcgccgcat ctccgcgacg gccaggtgct gttcctgccc ccgggcacgt tcggcagcta
     2041 cgtgatggcg cgcgcgatcg ccgacgccgg cggcgccgcg atcgcgatcg ccgaggccgg
     2101 gacgctgccg tggctggcgc gcaagcgcgg cccgcgcgag gtcgcgatca gcgcccgcgc
     2161 cacccgcctg cccgcgggcg tgttcccggc cgcgcgcgcg ggcgccgcgt tcgaggtcct
     2221 gcaggccgcg ctgccggcgg tcgagccgct ggccgacgcg ctcgacggcg cactggtcaa
     2281 tgccgggccg atcatccacc cgccgctgat cgtgatgaac gccggcccga tcgagcattt
     2341 cgagcgctgg gacatccacg ccgaaggcac ccaggcgtcg atccgccggg tgaccgatgc
     2401 gctcgacgag gagcgggtgg cggtacgcga ggcgctcggc tacggcgccc cgcactaccg
     2461 cctgcgcgac cattacgcgg tctcgggcga ggaatggatg tacggcaact ccgcccacga
     2521 gcgcctggtc gacagcggcg actggcgcga gccgatcgac ctcgccggcc accgctacat
     2581 gcgcgaagac gtcgcctgcg gcctcgcctt cctggtctcg gtagcgcgct gggccggggt
     2641 ggcggcgccg gtcgcgggcg ggctgctggc gatcgcctcg gcgatgctcg gcgaggacct
     2701 ccgccggacc ggccggaccc tcgaagcgat gggcctcgcc cgcttgtcgc gcgccgagat
     2761 gcgcgccctg ctcgccgacg gcccgggcgg cgcgtgagcg accggcccgc ggtcgcgatc
     2821 gtcggcgccg ggcgcatggg ccgcggcctc gcgcatgtgt tcgcctatgc cggccaccgg
     2881 gtcgccgtga tcgacctcaa ggcgcggccg aaggacgaac gccggcgcct cgccgacgac
     2941 gcccgcgccg aggtccgccg cagcctggag gccctcgccg atcacggcgc gctgccgatg
     3001 gcgagggtcg agccgctgct ttcccgggtc accattgccg gggcggccga ggccggcgag
     3061 gcgctggcag cggccgggtt cgtgttcgag gcggtgcccg aacggctgga tgcgaagcgc
     3121 gcggcgctcg gcgtcatcgg ccgggctgcc gccgccgacg cggtggtcgc ctcggcgacc
     3181 tcgaccatgc gggtcgacga gcttgcagcg ctgatcgagc ggccggagag gttcgtcaac
     3241 gcccactggc tcaaccccgc ctacctggtg ccgctggtcg aggtgagccc gggccgcgcc
     3301 accagccccg acactttggc ccggctgctc gcgctgctcg aagcggtcgg caaggtggcg
     3361 gtggtgtgcg ccgcctcgcc cggcttcatc gtgccgcgcc tgcaggcgct ggcgatgaac
     3421 gaggccgcgc gcctggtcga ggaaggggtg gcaagccctg aggacatcga taccgcgacc
     3481 cggttcgggc tcggctttcg gttcgcggcc atgggggtgg tcgagttcat cgactggggc
     3541 ggcggcgaca ttctctatta cgccgacgag tacctgcgcc aaagcctggg cgccgagcgc
     3601 ttcgccgctc ccgacatcgt gcgccgcaac atggaggcgg gcgccgtggg cgtgcgcgcc
     3661 gggcgcggct tccacgactg gcaggggcgc gacctcgacg cctaccagcg cgagaccatc
     3721 gcccgcttcg tggcgctgct ccgccactac ggcctgctca agccgcccgc cgaggactga
     3781 gggcgccgga taagcattga aaatgagatc gcgtctcaat atcattcgat cccgggaaac
     3841 gacccctaag cacccgacgt ctcggggagg cagacgatga caggacggac ctggccgcgc
     3901 ctcgcggcca tgctggcgat ggcggtgatg ccgccggccg gggcgttggc ggccgaggac
     3961 gaggccaccc cgcccgcgtc ctacgcgctc gagacgagcg actggagcga ggccgcctat
     4021 gacgcgcgcc ctcagatcga gagctacgcg atgctggtgc gggcgagcta cgccaaggcg
     4081 gcggcggacg cagcggccct g
//
LOCUS       DASWHU010000216        18503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_844555, whole genome shotgun sequence.
ACCESSION   DASWHU010000216 DASWHU010000000
VERSION     DASWHU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18503
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844555"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGB88_07925"
     CDS             <1..471
                     /locus_tag="VGB88_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX9647411.1"
                     /translation="PYGTETMHLLRAEKGYIMVGQESDGTVTPIDLGLERMIGRTKAD
                     FIGKRSLARPDLVRPDRKQLVGLATEDGAVVLEEGVHLVAAERLPRPPVPALGHVTSS
                     YFSPNLGRSIALGLVRGGRARMGQRLYAATPEGQVPVIVTDPVFLDPDGTRLDG"
     gene            494..1072
                     /locus_tag="VGB88_07930"
     CDS             494..1072
                     /locus_tag="VGB88_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX9647412.1"
                     /translation="MSAPAPGAAEPGVRLAEVAHAGKLVLRGRAEPEFIEAAASVGLS
                     LPTEPCTAATTEAGAALWLGPDEWLIVTVPGAEGALAASLHAALAGLPAAVVDVTDSR
                     TMLRVSGHAARAVLAKGCALDFHPRGFAAGAVKQSLLAKVDVVIRRPSGAPDDADPVF
                     EVTVLRSFADHLWRWLVDAATEYGLAQPRAPE"
     gene            1148..1603
                     /locus_tag="VGB88_07935"
     CDS             1148..1603
                     /locus_tag="VGB88_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9647413.1"
                     /translation="MAIGLTVNGKSYEVDAPPQMPLLWVLRDIIGLTGTKFGCGIAQC
                     GACTVHIDGVPVRSCTTAVGSVQGKVTTIEGLGASGDHPLQKAWIKNNVPQCGYCQSG
                     QIMQAAALLAKTKKPTDDDIDAAMAGNICRCGTYQRIRAAIKAAAEDMA"
     gene            1600..3900
                     /locus_tag="VGB88_07940"
     CDS             1600..3900
                     /locus_tag="VGB88_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9647414.1"
                     /translation="MSALENVSRRTFIKGVAGTGGLVLGYKLLPVVGKLPRARAAGAT
                     FAPSLFVSIAGDGTTTITCHRSEMGQGIRSGLPMVVADELEADLARVVVVQAEGDEKY
                     GGQYTDGSHSMVEFYDQMRQLGAAARTMLEQAAAKQWGVDAASCRAKNHRVVEVKSEK
                     RGESMIEVETGRSADFGDLVETAAALPVPADDAVTLKRSSQFRYIGTDIPLVDGPDMA
                     RGKATYTIDITMPGMKYASVERCPVALGRVKSYDASAAMKVPGVIAVVEIPPPVKPVL
                     FQALGGLSVVAENTWAAMEGRKKLKIEWDLGENAGYESVAYRKAMEASARAKGDVRRE
                     QGDVEAAFGQAAKVIEAEYYSPHFVHAPMEPPGAVANVHDGVCEVWSSTQDPQTARTV
                     VAEALGIDAANVTSRMPLLGGAFGRKSKPDFAVEAALSSKAVGAPVKVTWTREDDIRN
                     GYYHSAAAQYLKAGLDGDGKVTAWLHRSVFPPIESIFTADAAKPASWELDFGMVDLPY
                     DISNLAMETGEAAAHVRIGWLRSVQNIFHAFAIGSFVDEIAHAAGRDPVDNLLGLIGP
                     ARHVDLKLAGEDVEYSNYLFPIDKYPIDTGRLRKVVEVAADKSGWGRKLPARHGMGIF
                     AHRSFLTYVATVVEVAVAKDGTVSIPRVDMAVDCGLAVNTDRVRAQMEGATIYGMSAA
                     CYGEITAKDGRIQQGNFDSYHVVRMNDAPRQTNVHVLQNMDAPPSGVGEPGVPPFAPA
                     LCNAIFAATGIRVRSLPIMNTDLSET"
     gene            complement(3997..5241)
                     /locus_tag="VGB88_07945"
     CDS             complement(3997..5241)
                     /locus_tag="VGB88_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9647415.1"
                     /translation="MSYTTALVVAVFLAVYAGMALGRWPGLKIDRTGIALLGAIVLYA
                     SGTVSGAAALAAIDFPTLIVLFGLMILSAQFAACGFYDWCSARIAATRAAPAAILGLT
                     VLIAGVLSAVLANDVVVFAMTPMLVKGLRRRGLDARPYLIALAGAANAGSAATVIGNP
                     QNILIGEVGGLDFWDFALVCAPPALFGLIAVFAVVWLVWRGRLRSADAGDAAAEIPPL
                     DRFGLAKATVATIALLAMFATPLPHVSGVLVIAAVLLVSRRLATREILGLVDWHLLAL
                     FAGLFIVTAALAATGLPAEALAALAAAGIEPTGLAALAPLMLAGSNTIGNVPTVVLVL
                     AILQDLPAETLYLLAVLSTLAGNLLIVGSIANIIVVERARQAGVALGFGTHARCGVPM
                     TLLSFAAAIAWFAGGALLGRGA"
     gene            complement(5353..5679)
                     /locus_tag="VGB88_07950"
     CDS             complement(5353..5679)
                     /locus_tag="VGB88_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9647416.1"
                     /translation="MVFLLALLAAMPPAAAAAGGDDEWANPVLGDPQAIEDGGKLFRA
                     RCTGCHWNPLRGPRIFQTKRTYEKFLEIVINGKKTVRTMPPFGYLLTPDDISKIHAYL
                     MSRERP"
     gene            complement(5760..7604)
                     /locus_tag="VGB88_07955"
     CDS             complement(5760..7604)
                     /locus_tag="VGB88_07955"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX9647417.1"
                     /translation="MLRSPASLALRVAMVAAPALLWSSAQAGPVTSDDLLNAEGNTSE
                     WLQYSRTYDGHRFVRLSQINRSNVHRLRPSWIFPVGGENRGLEATPLIHDGVIYLSAD
                     ESRVFAIDAHTGAKIWAFDPKVAEEAERVYCCGSQNRGVALLGNMVFVGTMDARMVAL
                     NKDTGEAVWDKVVFDWQAGYSITGAPLVVKDMVLTGVAGGEFGIRGFVKAFDAKTGAE
                     RWTTYAIPGPGEPGNETWPGDTWKDGGGSTWTTGAYDPELDLVYWQVGNAGPWNCQLR
                     KGDNKWTAGTIALDADNGKILWGFQYTPWDCYDYDSVSTPVLADLSIGGAMRKALLHH
                     DKNGFFYAIDRTNGDFIYGKPVVPGLNWAKGLDPKTGRPIVNEDFIAQTGGPEVAPII
                     PSLEGAIDWQPLAYNPDNGTIFFMSNTWAMGYKFYEECARPTGAGEWYLCADYQEYVR
                     PDIPVGAFVAIDVATGAEKWRTTNDRPFWAGAVATSGGLVFTGDNYGNALAMDAHTGT
                     VLWRFPTGSPISGSPITYDLDGVQYVLVPSGMAGDLTFYYTNPKAANLIAFALDGGGT
                     PPLPIADNLEYVPGGLPMVGEPGMFLGGRVIEGYGYPPMDERDSIKSE"
     gene            7948..8628
                     /locus_tag="VGB88_07960"
     CDS             7948..8628
                     /locus_tag="VGB88_07960"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9647418.1"
                     /translation="MAMTGAALAVGPGLTAPDFALPRVEPEGQVALADYRGRAPLLLA
                     LFRSFECPFCRRLMAALKTTAGEFHQFGIETLGVTTTPVRAARLYARYRPPGLTLASD
                     PSLGVHRAYGVPIFRFATDQPTDWPRRINVADINRLRLNPTGELPEALPGIEASRALD
                     RIDGFEMIRTDERFPPEDVSPLSAYFLIDRAATVRWRFIEALHDPAEYGRHPGAAELL
                     AAARSLSS"
     gene            8775..8933
                     /locus_tag="VGB88_07965"
     CDS             8775..8933
                     /locus_tag="VGB88_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647419.1"
                     /translation="MVRVIEDLIKMLIDKNVIAADDLPEAARSKLLRRHIWRRRMTPA
                     GKPSPDDD"
     gene            8973..9830
                     /locus_tag="VGB88_07970"
     CDS             8973..9830
                     /locus_tag="VGB88_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX9647420.1"
                     /translation="MARHLVCLTFDFDALSGWIARGLVTPTPISRGEFGVVGAARLLA
                     LLAKHDIRATWFIPGVTIATYRDSCKAVVDAGHEIGNHGWTHVPPAKLSRVEEAAGLA
                     RANEAIAALAGRAPQGYRSPSWDLSPHTVELLIEHGFAYESSMMGHDYLPYRARKGDV
                     IALDEAVSFGPETELVEMPISWSLDDYPHFEFVRTETTLLPGLMNARDVIENWIDDFT
                     YMARHYEWGVLTYTCHPFVIGRGHRMLAFERLIEALKVQGAVFVTMAEAVAEWRAQGE
                     PRNRGVFAP"
     gene            complement(9987..11099)
                     /locus_tag="VGB88_07975"
     CDS             complement(9987..11099)
                     /locus_tag="VGB88_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647421.1"
                     /translation="MIRFGWLVGLVAALAAGLYIGANFMASPAGVPEPGLAARMEAAA
                     PPAPKTVEWKLASTFFKSWPIHGDLGQLLVKNVAIATGGGVELKFFEPGALVPTLEVF
                     DAVSTGSVDAGWSSPGYWAGKEPVMPIFTTVPFGPNMNEYLAWLYAGGGEELFREVYA
                     KHNIYPLHCGLHSPEGSGWFRKPIEGPEDLKGLKMRFFGLGARVMEKLGVSTQLIAGG
                     DVYPALELGTIDALEFSMPISDLNQGFWQVAKHYYFPGWHQQTSLFELIVNMDRWNEL
                     SELQKQQITLACSDLFRAGAAMSESMQVEALEQLKEKGVILHKWPPEMIEMFRKATDE
                     VMAEEAAKDETFARVWQSLSTFRASYAEWRDLGYLN"
     gene            complement(11318..12007)
                     /locus_tag="VGB88_07980"
     CDS             complement(11318..12007)
                     /locus_tag="VGB88_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647422.1"
                     /translation="MAALDRGLFVSKGAARPAPDYVLHLKTEEPARLDLTAAPPPAPT
                     SGGGGLGGLIRRSVAPRQLPEQQVAYERSREHAATVELEAETLRRTIAEQVDNGLMYE
                     SLLRGLPMPGVQLARRQGTEMPAEPSTFEEAARERISRPTRPDGTGAERRKAARRRKL
                     TARLKMRDFTRFKRYADETGRTYQDVLAAATVSYLDWVSTKEGDPLQDEPEDFDVILD
                     GDDAGDDSDPG"
     gene            complement(12053..12793)
                     /locus_tag="VGB88_07985"
     CDS             complement(12053..12793)
                     /locus_tag="VGB88_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9647423.1"
                     /translation="MQVLVFTSQKGGSGKTTLCGHLAVAAELSGHGPVAMVDCDPQGS
                     LADWWNARAAHTPLFVSTNVAKLGDTLKMLKMQGITLVFIDTPPSVTNTIRDIVGHAD
                     LVVIPTRPSPHDLRAVGATLDIVDTQGKPLVFAVNGATKRAKITGDTAIALSQHGTVA
                     PVTLHHRNDFATSMIDGGTVMEANPSSNSAAEVVELWRYLHSRLRKERRPGPRSLTGE
                     RRDPARARPTFGGAMQRPGTTFGRRAYD"
     gene            13316..13666
                     /locus_tag="VGB88_07990"
     CDS             13316..13666
                     /locus_tag="VGB88_07990"
                     /inference="COORDINATES: protein motif:HMM:NF040013.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HEX9647424.1"
                     /translation="MKVYSGRRIAGTCEVTVDGVPLDPRLDLRVFSAAGFEWGYDGAG
                     PRQLALAIVADHFADVQVALGQHDRFRLNFVVAMRDDSWSLTSREVERALNEVIEVPM
                     TLEELFDKVRGRPR"
     gene            13663..14058
                     /locus_tag="VGB88_07995"
     CDS             13663..14058
                     /locus_tag="VGB88_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEX9647425.1"
                     /translation="MSARRLRMTIGTVTLDAELLDTPTADAIYHALPFDARASTWGEE
                     VYFATPVRAALEAGARAVVEAGELAFWVEGDAIAIGFGATPISRGAEIRLASPTNIWG
                     RALGDVRTLTAVRAGARVRVEALPAAGDG"
     gene            14051..14359
                     /locus_tag="VGB88_08000"
     CDS             14051..14359
                     /locus_tag="VGB88_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9647426.1"
                     /translation="MAETLLTRGRFAAPVDADRVAADWRARGFDCRPFTDPPGREWND
                     FVHPTNELVTVLDGRLEMTVGERSLIVEPGDEVLIPAGARHSVRNLHRGTTRWLFGYD
                     "
     gene            14493..15461
                     /locus_tag="VGB88_08005"
     CDS             14493..15461
                     /locus_tag="VGB88_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647427.1"
                     /translation="MLDRFPVAAGDFDGPVLVTGAGGCIGAWTLALLSRNRVPAVAFD
                     LSADTRRPGLLIAEDELAAIPWRVGNIADTDAVIAAVADHGVRAIVHLAALQVPFCIA
                     DPVAGARANVVGTVNVLEAARRHGIRRVAYASSIAAHGLLGDGWLATLYGAYKLCDEM
                     IARVYAQDWGVPSVGLRPGVVAGVGRDQGLTSKTTQAILAAAAGRPYEVPFTGPVSFL
                     HAGEIAWTFIAAVSAERDGAPVFDVNGRATTVEDILSAIRALAPDARLTSRGAPLPFP
                     ADLPDAPVRAFLGAGAPVPVAEGVGETLRAFRALLEAGKLSPDAIA"
     gene            complement(15482..15769)
                     /locus_tag="VGB88_08010"
     CDS             complement(15482..15769)
                     /locus_tag="VGB88_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647428.1"
                     /translation="MTRRRRLAAAAAALLALAPAPAAAGDGMFLLCYERMKQMIALIV
                     VQGGHVEEVEDDPEARAYRAVIRQPSGDKEVWCSPGGEIMVEAADAPPPAD"
     gene            complement(15766..16527)
                     /locus_tag="VGB88_08015"
     CDS             complement(15766..16527)
                     /locus_tag="VGB88_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647429.1"
                     /translation="MDLGLAGLKAIVTGGTRGIGRAVVERLLAEGCEVALCARDAAGV
                     EATVAALSARGPRVIGTAVDVGDGVLLADWVAASAERLGGLDIVIANASALAVALTDQ
                     AWAAGFSVDLMGTVHAVDAALPHLRRSEAGAIVAVASTAALEIYAGLRSYNSFKAALI
                     AYIGGLAIELAPEHIRANSVCPGSIYSDQGTWQRAERDNPDAFRAMLARNPMGRMGRP
                     EEMANAIAFLASPAASFITGANLVADGGLTRRIQY"
     gene            complement(16583..17101)
                     /locus_tag="VGB88_08020"
     CDS             complement(16583..17101)
                     /locus_tag="VGB88_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX9647430.1"
                     /translation="MSPDWLGWPAAPEQAREVEMSGPSHALKDKAPPSEHSLADVLRK
                     AEDKLADLVDDYLEWAREDAERLDAACRELEASPGARPLRDALYRIAHDMKGQGGTFG
                     YPLVSMIGDSLCKLLERAPVLDRAVVAAARLHVDALRSVIAQRVIGDGGEAGVELVAG
                     LAKVAAKASGGR"
     gene            complement(17098..17646)
                     /locus_tag="VGB88_08025"
     CDS             complement(17098..17646)
                     /locus_tag="VGB88_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9647431.1"
                     /translation="MRTILNLSAVSFLVVDDSRHMRAIIRYVLRLFGARTIREAEDGA
                     SALEVLASFTPDIVITNWLMAPVDGIELTQTLRRAPDSPCPTVPIVMISGHTEEARVA
                     EARDAGVTEFLAKPMTPKALYMRIEEVILKPRRFVRTPDYVGPDRQRHGDDGYAGPDR
                     RKIERGAEEPEAASASPRETAA"
     gene            18051..>18503
                     /locus_tag="VGB88_08030"
     CDS             18051..>18503
                     /locus_tag="VGB88_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647432.1"
                     /translation="MARSVTDTKLSTRTGRLQLAPRGKPYYRELEQGLHLGYRRLKRG
                     AGRWVARVYVGNEQYRAEVIGTADDYGDAAPDDDPDRARILDYKQAQEAARRAADRIH
                     LDPGASLEGVGAEQPAGNGRFTVERALRDYLKAMEHRGQNAADSRARAE"
BASE COUNT         2738 a         6479 c         6556 g         2730 t
ORIGIN      
        1 ccctacggca ccgagaccat gcacctgctg cgcgccgaga agggctacat catggtcggc
       61 caggagtccg acggcaccgt caccccgatc gacctcgggc ttgagcggat gatcggcagg
      121 accaaggccg acttcatcgg caagcgctcg ctcgcgcgcc cagacctggt gcgccccgac
      181 cgcaagcagc tggtcgggct cgccaccgag gacggcgcgg tggtgctcga ggaaggcgtc
      241 caccttgtcg cggccgagcg cctgccgcgg ccgccggtgc cggcgctcgg ccacgtcacg
      301 tcgagctatt tcagccccaa cctggggcgc tcgatcgcgc tcggcctggt gcgcggcggg
      361 cgcgcgcgga tgggccagcg cctgtacgcg gcgacgcccg aaggccaggt cccggtcatc
      421 gtgaccgacc cggtgttcct cgaccctgac gggacgcgac tcgatggctg aacgccgcgc
      481 agcgctgtct catgtgagcg cgcccgcacc cggcgcggca gagccagggg tgcgcctcgc
      541 cgaggtcgcc catgccggca agctggtgct gcgcgggcgc gccgagcctg aattcattga
      601 ggccgcggcc tcggtggggc tgtcgctgcc gaccgagccg tgcacggccg cgacgacgga
      661 ggccggcgcc gcgctgtggt tggggcccga cgagtggctg atcgtcaccg tgcccggcgc
      721 cgaaggcgcg ctggccgcct cgctgcacgc tgcgcttgcc ggcttgcccg cggcggtggt
      781 cgacgtcacc gacagccgca ccatgctccg cgtctcgggg cacgcggcgc gcgcggtgct
      841 ggccaagggt tgtgcactcg actttcatcc gcggggcttc gccgccggcg cggtcaagca
      901 gagcctgctc gccaaggtcg acgtggtcat ccgccggccg tcgggtgccc ccgacgacgc
      961 cgacccggtg ttcgaggtca ccgtgctgcg ctcgttcgcg gaccacctgt ggcggtggct
     1021 ggtcgatgcc gccaccgagt acggactcgc ccagccccga gcgcccgagt gaccaaagtt
     1081 tggcgtccaa agccgccggc aaccgtgcta ggattcgcgc ttctccagtc accaaggagg
     1141 gagggcaatg gctataggcc tcaccgtaaa cggtaagtcc tatgaggtcg atgcgccgcc
     1201 gcagatgccg ctgttgtggg tgctgcgcga catcatcggc ctgacgggca cgaaattcgg
     1261 ctgcggcatc gcgcagtgcg gtgcctgcac ggttcacatc gacggcgtgc cggtgcgctc
     1321 atgcacgacc gccgtcggct cggtacaagg caaggtcacg acgatcgagg gcctcggcgc
     1381 gagcggcgat catccgctgc agaaggcttg gatcaagaac aacgtgcccc agtgcggcta
     1441 ctgccagtcg gggcagatca tgcaggccgc ggcgctgctg gccaagacca agaagccgac
     1501 cgacgacgac atcgacgcgg cgatggccgg caacatctgc cgatgcggca cctatcagag
     1561 gatccgtgcc gccatcaagg cagcagcgga ggacatggca tgagcgcgct agagaatgtc
     1621 agccggcgca ccttcatcaa gggcgtcgcc ggaaccggcg ggctggtgct cggctacaag
     1681 ctgctgccgg tggtgggcaa gctgccgcgg gcacgggccg cgggcgcgac gttcgcgccg
     1741 agcctgttcg tgtcgatcgc cggcgacggc acgacgacca tcacctgcca tcgctcggag
     1801 atgggccagg gcatccgcag cggcctgccg atggtggtcg ccgacgagct cgaggcggac
     1861 ctcgcgcggg tggtcgtggt ccaggccgag ggcgacgaga agtatggcgg ccagtacacc
     1921 gacggctcgc acagcatggt cgagttctac gaccagatgc gccagctcgg tgccgcggcg
     1981 cgcacgatgc tcgagcaggc ggcggccaag cagtggggcg tcgatgcggc gagttgccgc
     2041 gccaagaacc acagggtcgt cgaggtcaag agcgagaagc gcggcgagag catgatcgag
     2101 gtcgagaccg gacgcagcgc cgacttcggc gatctggtcg agaccgcggc cgcgctgccg
     2161 gtgccggcgg acgacgcggt gacgctcaag aggtcgagcc agttccgcta catcggcacc
     2221 gacatcccgc tggtcgacgg gcccgacatg gcccgcggca aggcgaccta caccatcgac
     2281 atcaccatgc cggggatgaa gtacgcctcg gtcgagcgct gccccgtggc cctcggccgg
     2341 gtcaagtcgt acgacgcctc ggcggcgatg aaggtgccgg gggtgatcgc ggtggtcgag
     2401 atcccgccgc cggtcaagcc ggtgctgttc caggcgctcg gcggcctgtc ggtggtcgcc
     2461 gaaaatacct gggcggcgat ggagggccgc aagaagctca agatcgaatg ggacctcggc
     2521 gagaacgccg gctacgagtc ggtggcctat cgcaaggcga tggaagcctc ggcgcgagcc
     2581 aagggcgacg tccggcgcga gcagggcgac gtcgaggccg cgttcggcca ggcggccaag
     2641 gtcatcgagg cggagtacta cagcccgcac ttcgtgcacg cgccgatgga gccgccgggg
     2701 gcggtggcga atgtccacga cggggtgtgc gaggtgtggt cctcgaccca ggacccgcag
     2761 accgcgcgca cggtggtggc ggaggcgctc ggcatcgacg ccgccaatgt cacctcgcgc
     2821 atgccgctgt tgggcggcgc gttcgggcgc aagtcgaagc ccgacttcgc ggtcgaggcg
     2881 gcgctgtcgt cgaaggcggt gggcgccccg gtcaaggtca cctggacgcg cgaggacgac
     2941 atccgcaacg gctactacca ctccgcggcg gcccagtacc tgaaggccgg cctcgatggt
     3001 gacggcaagg tcaccgcgtg gctgcaccgc tcggtgttcc cgccgatcga gtcgatcttc
     3061 accgccgacg cggccaagcc cgcgtcgtgg gagctcgact tcggcatggt cgacctgcct
     3121 tacgacatat ccaacctcgc catggagacc ggcgaggcgg cggcgcatgt gcgcatcggc
     3181 tggctgcgct cggtgcagaa catcttccat gccttcgcca tcggctcgtt cgtcgacgag
     3241 atcgcccatg ccgccggccg cgacccggtc gacaacctgc tcggcctgat cggcccggcg
     3301 cgccacgtcg acctcaagct cgccggcgag gacgtcgagt actcgaacta cctgttcccg
     3361 atcgacaagt acccgatcga caccggccgg ttgcgcaagg tggtcgaggt cgcggcggac
     3421 aagtcgggct ggggccgcaa gctgccggcg cgccacggca tgggaatctt cgctcatcgc
     3481 agcttcctga cctatgtcgc gaccgtggtc gaggtggcgg tggccaagga cggcaccgtc
     3541 tcgatcccgc gcgtcgacat ggcggtcgac tgcggcctgg cggtgaacac cgaccgggtg
     3601 cgggcgcaga tggaaggcgc caccatctac ggcatgagcg ccgcctgcta cggcgagatc
     3661 acggccaagg acgggcgcat ccagcagggc aacttcgaca gctaccacgt ggtgcggatg
     3721 aacgacgcgc cgcgccagac caacgtccac gtcctgcaga acatggacgc gccgccaagc
     3781 ggcgtcggcg agcccggcgt gccgccgttc gctccggcgc tgtgcaacgc gatcttcgcc
     3841 gccaccggca tccgcgttcg cagcctgccg atcatgaaca ccgacctgtc ggagacctga
     3901 ccggcctgcg actcagcctt cgaaggcgcc gtggcgggac cgtcccgccc ggcgcctttt
     3961 ccttatcggt cggcaagggc ggtttcgatt gccaggctaa gcaccgcgac cgaggagcgc
     4021 tccgccggcg aaccaggcga tggcggcggc gaacgacagc agggtcatcg gcaccccgca
     4081 gcgggcatgc gtgccgaagc cgagcgcgac gccggcctgc ctggcgcgct cgacgacgat
     4141 gatattggcg atgctgccga cgatcagcag gttgccggcg agcgtcgaca gcaccgccag
     4201 caggtacagc gtctcggcgg gcaggtcctg gaggattgcc agcaccagca ccaccgtcgg
     4261 cacgttgccg atggtgttgc tgccggcgag catcaacggc gcgagggccg caaggccggt
     4321 cggctcgatg ccggcggcgg cgagcgcggc cagggcctca gccggcagcc cggtcgcggc
     4381 cagcgccgcg gtgacgatga acaggccggc gaacagcgcc agcaaatgcc agtcgaccag
     4441 gccgagaatc tcccgcgtcg ccaggcgccg gctgaccagc agcacggcgg cgattaccag
     4501 cacgcccgag acgtgcggca gcggtgtcgc gaacatcgcc agcaaggcga tcgtcgccac
     4561 cgtcgccttg gcgaggccga agcggtcgag cggcggtatc tctgctgcgg cgtcgccggc
     4621 atcggccgag cgcaaccggc cgcgccagac cagccagacc actgcgaaca ccgcgatcag
     4681 gccgaacagc gcgggcggtg cgcagaccaa cgcgaagtcc cagaagtcga gcccgccgac
     4741 ctcgccgatc aggatgttct gggggttgcc gatcacggtt gcggccgagc cggcgttggc
     4801 cgcgccggcg agcgcgatca ggtaggggcg ggcatcgagg ccgcgacgtc tgagcccctt
     4861 gaccagcatc ggcgtcatcg cgaacacgac cacgtcgttg gcgagcaccg ccgacaggac
     4921 gcccgcgatc agcaccgtga gtccaaggat ggcggccggc gccgcgcggg tcgccgcgat
     4981 cctggccgag caccagtcgt agaagccgca ggcggcgaac tgggccgaca ggatcatcag
     5041 cccgaacagc acgatcaggg tcgggaagtc gatcgcggcc agcgccgccg cgccgctcac
     5101 ggtccccgac gcatacagga cgatcgcgcc gagcagcgcg atgccggtgc ggtcgatctt
     5161 cagccccggc cagcggccga gcgccatgcc ggcgtacacg gcgaggaaca ccgccaccac
     5221 cagcgccgtc gtgtaggaca tcggttgttc gaccccgccg ccgcgaaccc gcctttgcgt
     5281 ggctgtcaac gctcgcttcg gccgactggg aaaaacggct ggtggctcag gctcccatgc
     5341 ctccgcccgc cctcacgggc gttcccggga catgaggtag gcgtgaatct tcgagatgtc
     5401 gtcgggcgtg agcaggtagc cgaacggggg catggtgcgc acggtcttct tgccgttgat
     5461 cacgatctcc aggaacttct cgtaggtccg cttggtctgg aagatgcgcg ggccgcgcag
     5521 cgggttccag tggcagccgg tgcagcgcgc gcggaacagc ttgccgccgt cctcgatcgc
     5581 ctgcggatcg ccgaggaccg gattggccca ctcgtcgtcc ccgcccgccg ccgcggccgc
     5641 cggtggcatc gcggcgagca gcgcgagcag gaagaccatg agcccggcga gcgacggagc
     5701 ggtatgagag tgtgctcgct gtcgttcgac cgggctcatg cttgagacgc ggcgtcggcc
     5761 tactcggact tgatcgagtc ccgctcgtcc atcggcgggt agccgtagcc ctcgatgacc
     5821 ctgccgccga ggaacattcc gggctcgccg accatcggca ggccgcccgg gacgtactcg
     5881 aggttgtcgg cgatcggcaa cggcggcgta ccgccgccgt cgagcgcgaa ggcgatcagg
     5941 ttcgcggcct tcgggttggt gtagtagaag gtgaggtcgc cggccatgcc cgaggggacc
     6001 agcacgtact gcaccccgtc gaggtcgtag gtgatcgggc tcccggagat cggcgagccg
     6061 gtcgggaagc gccacagcac ggtgccggtg tgtgcgtcca tcgccaaggc gttgccgtag
     6121 ttgtcgccgg tgaacaccag accgcccgag gtggccaccg cgccggccca gaacggccgg
     6181 tcgttggtgg tgcgccactt ctcggcgccg gtggcgacgt cgatggcgac gaacgcgccg
     6241 accggaatgt ccggacgcac gtactcctga tagtccgcgc acaggtacca ctcgcccgcg
     6301 ccggtcggac gagcgcactc ctcgtagaac ttgtagccca tggcccaggt gttcgacatg
     6361 aagaagatcg tgccgttgtc cgggttgtag gcgagcggct gccagtcgat cgcgccctcc
     6421 aggctgggga tgatcggtgc cacctcgggg ccgccggtct gggcgatgaa gtcctcgttc
     6481 acgatcggcc gcccggtctt ggggtccagg cccttggccc agttcagccc gggcaccacc
     6541 ggcttgccgt agatgaagtc gccgttggtc cggtcgatcg cgtagaagaa gccattcttg
     6601 tcgtggtgca gcagcgcctt gcgcatcgcg ccgccgatcg agaggtcggc gaggaccggc
     6661 gtggacaccg agtcgtagtc gtagcaatcc cacggcgtgt actggaagcc ccacaggatc
     6721 ttgccgttgt cggcgtcgag cgcgatggtg cccgcagtcc acttgttgtc gcccttgcgc
     6781 agctggcagt tccacggccc ggcattgccg acctgccagt agacaaggtc gagctcgggg
     6841 tcgtaggcgc cggtggtcca ggtcgacccg ccgccgtcct tccaggtgtc gcccggccag
     6901 gtctcgttgc ccggctcgcc gggtcccgga atcgcgtagg tcgtccagcg ctcggccccg
     6961 gtcttggcgt cgaacgcctt gacgaagccg cggatgccga actcgccgcc ggcgacgccg
     7021 gtgagcacca tgtccttcac caccagcggc gcgccggtga tgctgtagcc cgcttgccag
     7081 tcgaacacca ccttgtccca gacggcctcg cccgtgtcct tgttgagcgc gaccatgcgg
     7141 gcgtccatgg tgccgacgaa caccatgttg ccgaggagcg ccacgccgcg gttctgggag
     7201 ccgcagcaat agacgcgttc cgcctcttcc gcgaccttcg ggtcgaatgc ccagatcttg
     7261 gcgccggtgt gggcgtcgat cgcgaacacg cgcgattcgt ccgccgacag gtagatcacc
     7321 ccgtcatgaa tcagcggcgt cgcttccagg ccgcggttct cgccgccgac cgggaagatc
     7381 cacgacggcc gcagccggtg aacgttggag cgattgatct ggctcaatcg aacgaagcgg
     7441 tggccgtcat aggtgcggct gtactgcagc cattccgagg tatttccttc tgcgttcagc
     7501 aggtcgtcgc tggtcaccgg gcccgcctgg gccgacgacc acagcagcgc cggcgcggcc
     7561 accatggcca ccctcagcgc aagcgaggct ggtgatctga gcattcttcc cctcccgttg
     7621 gatcaggcca tggctcggcg cgcgccgagc ccgctcaaaa ggcttcgccg ccgcccgccc
     7681 tgggtccgcg accggctgcc ggatgaccgc gccgccgggc acgaacgaac gcgcaaggcc
     7741 gcattaaacg cgccgagccg ccccggcgtc aaggaaggtc cgggcggtcg cgaagcgccg
     7801 aaaaacgggg cgcggcgggc ggtccctgca ggtcgcgaag ggcccgccgc ggagtctttg
     7861 ccggcgggct cgtcgacgat ccgtcgcact cgcgaccggg cgggggtcat gctaggcttg
     7921 gcgcggactt gtgacgaggt gtcgatcatg gcgatgacgg gcgccgcact tgccgtcggg
     7981 ccgggactca cggcccccga tttcgcgctg ccgcgggtcg agcccgaagg gcaagtggcg
     8041 ctcgccgact accgcggccg ggcgccgctg ctgctcgccc tgttccgcag cttcgagtgc
     8101 ccgttctgcc gccgcctgat ggcggcgctc aagaccaccg ccggcgagtt ccaccagttc
     8161 gggatcgaga ccctcggcgt caccacgacg ccggtgcgcg cggcgcgcct ctatgccagg
     8221 taccgcccgc ccggcctgac gctggcatcg gacccttcgc tcggcgtcca tcgcgcctac
     8281 ggcgtgccga tcttccgctt tgccaccgac cagccgaccg actggccgcg gcggatcaac
     8341 gtggccgaca tcaaccggct gcggctcaac ccgaccggcg agctgcccga ggcgctgccc
     8401 gggatcgagg cgagccgggc attggacagg atcgacgggt tcgagatgat ccgcaccgac
     8461 gagcggttcc cgcccgagga cgtctcgccg ctgtccgcat acttcctcat cgaccgcgcg
     8521 gcgacggtgc gctggcgctt catcgaggcg ctgcacgatc cggccgagta cggccgacac
     8581 cccggcgcgg ccgagctgct cgccgccgcc cgatcgctgt cgtcataacg cggttcgctg
     8641 cggccgcccc cggcaggctt gttggcacga caatcgcgtg gaacaatcag gcgcgcacgc
     8701 cttggcgcgt gtgaccctcg agcggcgaga tgacggaaga ccctcgcaag agtctggctg
     8761 aatcggatca ggagatggtg cgggttatcg aggacctgat caagatgctg atcgacaaga
     8821 acgtcatcgc agccgacgac ctgccggagg cggcccgctc gaagctgctc aggcgccata
     8881 tctggcgacg gcgcatgacg ccggccggca agccgagccc ggacgacgac tgagcggcgc
     8941 agcgccgcgc tgaccatcca cggaggctcg gattggcccg acatctggtg tgccttactt
     9001 ttgacttcga tgcgctgtcg ggctggatcg cgcgcggcct ggtgacgccg acgccgatct
     9061 cgcgcggcga gttcggcgtg gtcggcgcgg cgcgcctgct ggcgctgctc gccaagcacg
     9121 acatccgcgc gacctggttc atccccgggg tcaccatcgc cacctatcgc gactcgtgca
     9181 aggcggtggt cgacgccggc cacgagatcg gcaatcacgg ctggacgcat gtgccgccgg
     9241 cgaagctgtc gcgcgtcgag gaggcggcgg ggctcgcgcg cgccaacgag gcgatcgccg
     9301 cgctcgccgg gcgcgcgccg cagggctacc gctcgccgtc ctgggacctg agcccgcaca
     9361 cggtcgagct cctgatcgag cacggcttcg cctacgagag cagcatgatg ggccacgact
     9421 acctgcccta ccgcgcgcgc aagggcgacg tcatcgcgct cgacgaggcg gtgtcgttcg
     9481 ggcccgagac cgagctggtc gagatgccga tcagctggtc gctcgacgac tacccgcatt
     9541 tcgagttcgt gcgcaccgag accacgctgc tgccgggcct gatgaacgcg cgcgacgtga
     9601 tcgagaactg gatcgacgac ttcacctaca tggcgcggca ctacgagtgg ggcgtgctca
     9661 cctatacctg ccacccgttc gtgatcggcc gcggccaccg catgctcgcc ttcgagcggc
     9721 tgatcgaggc gctgaaggtc cagggcgcgg tgttcgtgac catggcggag gcggtggccg
     9781 aatggcgggc gcagggcgag cccaggaacc gcggcgtgtt cgcgccgtga cgtcccgccg
     9841 tctcagggcc cgaaaatctt ttgcctcacc cttcgagacg cgcccgtcgg gcgctcctca
     9901 gggtgaggta aatgattgat tctcaacccc cctcgtcctg aggagcgagc gtctcgaagg
     9961 gcgacagccg attcattcac agccgctcag ttcaggtagc cgagatcgcg ccactcggcg
    10021 taactcgcgc ggaacgtgct cagcgactgc cagacccgcg cgaaggtctc gtccttcgcc
    10081 gcttcctcgg ccatcacctc gtcggtcgcc ttgcggaaca tctcgatcat ctcgggcggc
    10141 catttgtgga ggatcacgcc cttctccttg agctgctcga gcgcctcgac ctgcatcgat
    10201 tcggacatcg ccgcgccggc gcggaacagg tcggaacagg cgagcgtgat ctgctgcttc
    10261 tgcagttccg acagctcgtt ccagcggtcc atgttgacga tcagctcgaa caggctggtc
    10321 tgctggtgcc agcccgggaa gtagtagtgc ttggcgacct gccagaagcc ctggttgagg
    10381 tctgagatcg gcatcgagaa ctcgagcgca tcgatggtgc cgagctcgag cgccggatag
    10441 acatcgccgc cggcgatcag ctgggtggat acgccgagct tctccatcac ccgcgccccc
    10501 agcccgaaga agcgcatctt gaggcccttg agatcctcgg gcccctcgat cggcttgcgg
    10561 aaccagcccg agccctcggg cgaatgcagc ccgcaatgca agggatagat gttgtgcttg
    10621 gcatagacct cgcggaacag ctcctcgccg ccgccggcat agagccaggc gaggtactcg
    10681 ttcatgttcg ggccgaacgg gacggtggtg aagatcggca tcaccggctc cttgccggcc
    10741 cagtagccgg ggctcgacca gcccgcatcg accgagcccg tggacaccgc gtcgaacacc
    10801 tcaagcgtcg gcaccagcgc gccgggctcg aagaacttga gctcgacgcc gccgccggtg
    10861 gcgatggcga cgttcttgac caagagctgg ccgagatcgc cgtggatcgg ccacgacttg
    10921 aagaacgtgc tggcgagctt ccactccacc gtcttgggcg cgggcggggc cgcggcctcc
    10981 atgcgcgccg ccaaccccgg ctcgggcacg ccggccggcg aagccatgaa gttcgcgccg
    11041 atgtaaaggc cggccgcgag cgcggcgacc aggcccacca accaaccgaa tcggatcact
    11101 gctcgtctcc ccgtgacgtg gcgatcgccg cgcgtgcctg ccggccagcg cgcgggggag
    11161 tatagcagcc gatccgccgc aatggcgcct ggcaacgcgg cgcgggcggg cttgcgccgg
    11221 ggcgagcggt cacggcgggc cctgcggact gtcggaatcg aaccggcccg gcacgacgtg
    11281 ccgggcccgg cggggacggc cagcggtgcg cgccgcgcta tcccgggtcg ctgtcatccc
    11341 cggcgtcgtc gccgtccagg atgacgtcga agtcctcggg ctcgtcttgg agcgggtcgc
    11401 cctccttggt cgacacccag tccaggtagg acacggtggc cgcggcgagc acgtcctgat
    11461 aggtgcggcc ggtctcgtcc gcgtagcgct tgaagcgcgt gaagtcgcgc atcttgaggc
    11521 gcgcggtcag cttgcgacgg cgtgcggcct tgcgtcgctc ggcccccgtt ccgtcaggcc
    11581 gagtagggcg cgagatgcgc tcgcgcgccg cctcctcgaa cgtggatggt tccgccggca
    11641 tctcggtgcc ctgccggcgc gccagctgca cgcccggcat cggcagcccg cgcagcaggc
    11701 tctcgtacat caggccattg tcgacctgct ccgcgatcgt gcgccgcagg gtctcggcct
    11761 cgagctcgac ggtcgcggcg tgctcgcggc tgcgctcgta ggccacctgc tgctcgggca
    11821 gctggcgcgg cgccacgctg cggcggatca ggcctccgag gccgccaccg ccgctcgtcg
    11881 gcgcgggagg aggggcggca gtgaggtcga gccgcgccgg ctcctcggtc ttcaggtgca
    11941 gcacgtaatc gggcgccgga cgcgccgccc cttttgaaac gaacaggccc cggtcgagcg
    12001 cggccacgct gcgatccgtc atcttaccta tcctcctttg gccacaatcg actcagtcgt
    12061 aggcccgtcg gccgaaggtc gtgcctgggc gttgcatggc accgccgaac gtcgggcggg
    12121 cgcgcgccgg gtcgcgccgc tcgccagtga ggctgcgcgg ccccggccgt cgttccttgc
    12181 gcaggcggct gtgcagatag cgccacagct cgaccacctc ggccgccgag ttggagctcg
    12241 gattggcctc catcacggtg ccgccgtcga tcatgctggt ggcgaaatcg ttgcggtggt
    12301 gcagcgtcac cggcgcgacg gtgccgtgct gggacagcgc gatcgcggta tcgccggtga
    12361 tcttggcgcg cttggtggcg ccgttgaccg cgaacaccag cggcttgcct tgggtgtcga
    12421 cgatatccag cgtggcgccc accgcgcgca gatcgtgcgg gctggggcgg gtcgggatca
    12481 ccaccaggtc ggcatggccg acgatgtcgc gaatggtgtt ggtgaccgag ggcggggtgt
    12541 cgatgaacac cagggtgatg ccctgcatct tgagcatctt cagggtatcg ccgagcttgg
    12601 cgacgttggt gctgacgaac agcggcgtat gcgccgcgcg cgcgttccac cagtcggcga
    12661 ggctgccctg cgggtcgcag tcgaccatcg ccaccgggcc gtggcccgac agctcggccg
    12721 ccaccgccag atggccgcac aacgtggtct ttcccgagcc gcccttctgg gacgtgaaca
    12781 cgaggacctg catcggcagc gccaggtgcg ccggcgccac ggtgtggtcc gccgccgcgt
    12841 cgtcggccgg cgcggccgga tcggccggga cagccgggac gggatcggcc gcggggatca
    12901 ggttcgcggc cggcgccgtc ggccaggcga gcgcctccgc ggtcgggctc gtgccctgtg
    12961 ccggcgtcgc cggtaccccg ccgccgcctt ggcccggctc ctgtggcgca gccgtgaccg
    13021 gcgccggtgc cgtatcggcc cggacctcgg tctcggtccg ggtccggtct ggccccgaaa
    13081 agctaagtcc cagcatgatt ttccccatca aaccaatatc aacgccggca cctcaagggg
    13141 tgtccggtca cgcatggcgc aacgaccctt tcgagcccgt ctcggttgca gttagcgcgg
    13201 cttctgccct ggacttgtaa ccgcgatcac actttggccc ggcgcgggat gcgggcgtgg
    13261 cggccaaggt tgcggcaatg ctagacttta gtgcgaatgc ggccggaggc cagcgatgaa
    13321 ggtctacagc ggccggcgta tcgccgggac atgcgaggtg acggtcgacg gcgtgccgct
    13381 cgatccgcgc ctcgatttgc gggtgttctc ggccgcggga ttcgagtggg gctatgacgg
    13441 cgccgggccg cgccagctgg cgctggcgat cgtcgccgat catttcgccg acgtacaggt
    13501 cgcgctcggc cagcacgacc gtttccggct caacttcgtg gtcgcgatgc gcgacgattc
    13561 gtggtcgctg accagccgcg aggtcgagcg cgcgctgaac gaggtgatcg aggtgccgat
    13621 gacgctcgag gagctgttcg acaaggtcag gggccgaccg cggtgagcgc acgccggctg
    13681 cgcatgacca tcggaacggt gaccctcgat gccgagctgc tcgacacccc caccgccgac
    13741 gccatttatc acgccctgcc gtttgacgcg cgcgccagca cctggggcga ggaggtctat
    13801 ttcgccaccc cggtccgggc cgcgctggaa gcgggggcgc gcgcggtggt cgaggccggc
    13861 gagctcgcgt tctgggtcga gggcgacgcg atcgcgatcg gcttcggagc caccccgatc
    13921 tcgcgcgggg cggagatcag gctcgcctcg ccgaccaaca tctggggcag ggcgctcggc
    13981 gacgtgcgga cattgacggc ggtgcgcgcc ggcgcgcggg tgcgcgtcga ggcgttgccg
    14041 gccgcgggcg atggctgaga ccttgctcac ccgcggccgc ttcgccgcgc cggtcgatgc
    14101 cgaccgggtg gccgccgact ggcgcgcgcg cggcttcgac tgccggccgt tcaccgaccc
    14161 gccggggcgc gagtggaacg acttcgtcca tccgaccaac gagctggtca cggtcctcga
    14221 cggccggctc gagatgaccg tcggcgagcg cagccttatc gtcgagcccg gcgacgaggt
    14281 gctcatacct gcgggcgcgc ggcactctgt ccgcaacctg caccgcggca ccacccgctg
    14341 gctgttcggc tacgactgag aggctgggaa gcaaacgccg gacgatccct tcctcgccat
    14401 ttgcgcttgc gcgagggtga cgagatgggg ccgcggccgg ggagtccttc acaagcttcg
    14461 aggcgatcgc gcccggcagg gaggcaagac ggatgctcga caggttcccg gtcgcggccg
    14521 gcgacttcga tggtccggtt ctggtcaccg gcgccggcgg ctgcatcggc gcctggacct
    14581 tggcgctgct gtcgcgcaac cgggtgccgg cggtggcgtt cgacctgtcg gccgacacgc
    14641 ggcgtcccgg actgctgatc gccgaggatg agctcgccgc gatcccgtgg cgggtcggca
    14701 acatcgccga caccgacgcc gtgatcgccg cggtcgccga ccacggcgtg cgcgcgatcg
    14761 tccatctcgc cgcgctgcag gtgccgttct gcatcgccga tccggtcgcc ggcgcgcgcg
    14821 ccaacgtggt cggcacggtc aacgtgctcg aggcggcgcg gcgccacggg atcaggcgcg
    14881 tcgcctatgc cagctcgatc gccgcccacg gcctgctcgg cgacggctgg ctcgccaccc
    14941 tctacggcgc ctacaagctg tgcgacgaga tgatcgcccg ggtctatgcc caggactggg
    15001 gcgtgccgag cgtcggcctc aggcccggcg tggtcgccgg cgtcggccgc gaccaggggc
    15061 tgacctcgaa gaccacgcag gcgatcctgg ccgccgccgc gggccggccc tacgaggtgc
    15121 cgttcaccgg gccggtgtcg ttcctccatg ccggcgagat cgcctggacg ttcatcgccg
    15181 cggtgtctgc cgagcgcgac ggcgcgccgg tgttcgacgt caacggccgc gcgaccacgg
    15241 tcgaggacat cctgtccgcg atccgcgcgc tggcgccgga cgccaggctg acaagcaggg
    15301 gcgcgccgtt gccgttcccc gccgacctgc cggatgcgcc ggtacgcgcg tttctcggcg
    15361 ccggcgcccc ggtgccggtc gccgaggggg tgggcgagac gttgcgggcg ttccgcgccc
    15421 tgcttgaagc gggaaagctc tcgcccgacg cgattgcctg acgcgccgcg gcgggatcgg
    15481 gtcagtctgc gggcggcggc gcgtccgcgg cctcgaccat gatctcgccg ccgggcgagc
    15541 accacacctc cttgtcgccg ctcggctggc ggatcaccgc gcggtaggcg cgcgcctcgg
    15601 ggtcgtcctc gacctcctcg acgtggccgc cctggaccac gatcagcgcg atcatctgct
    15661 tcatgcgctc gtagcacagc aggaacatgc cgtcgcccgc cgcggccggc gccggcgcca
    15721 gagccagcaa cgcggccgcc gccgcggcca ggcgccggcg ccgggtcaat actggatgcg
    15781 ccttgtcagg ccgccgtcgg cgaccaggtt ggcgccggtg atgaagctcg ccgcggggct
    15841 ggccaggaag gcgatcgcgt tcgccatctc ctcgggccgg cccatgcgcc ccatcgggtt
    15901 gcgcgcgagc atggcgcgga acgcgtcggg gttgtcgcgc tcggcgcgct gccaggtgcc
    15961 ctggtcggag tagatcgagc ccgggcacac cgagttggcg cggatgtgct cgggcgcaag
    16021 ctcgatcgcg aggccgccga tgtaggcgat cagcgccgcc ttgaacgagt tgtaggacct
    16081 gagcccggca tagatctcga gcgcggcggt gctggcgacg gcgacgatcg cgccggcctc
    16141 ggagcggcgc agatgcggca gcgcggcgtc gaccgcgtgc accgtgccca tcaggtcgac
    16201 cgagaagccg gcggcccacg cctggtcggt gagcgcgacc gcgagcgcgc tggcattggc
    16261 gatgacgatg tcgaggccgc cgagccgttc ggcgctcgcc gccacccagt cggcgagcag
    16321 gacgccgtcg ccgacatcga ccgcggtgcc gatcacgcgc gggccgcgcg ccgacagcgc
    16381 cgccaccgtc gcctcgacgc cggcggcgtc gcgcgcgcac agcgcgacct cgcagccctc
    16441 ggcgagcagc cgctcgacca ccgcccggcc gatgccgcgc gtgccgccgg tgacgatcgc
    16501 cttcagcccc gccagcccga ggtccatggc gcagtatccg agcccgccga cccgccggca
    16561 aggcgagtcg cgggcggtcc gctcagcgcc cgcccgacgc cttcgccgcc accttggcca
    16621 ggccggcgac cagctcgacc ccggcctcgc cgccgtcacc gatcacgcgc tgggcgatca
    16681 ccgagcgcag cgcgtcgacg tgcaggcgcg ccgccgcgac cacggcgcgg tcgagcaccg
    16741 gcgcgcgctc gagcagcttg cacaggctgt cgccgatcat cgacaccagc ggatagccga
    16801 aggtgccgcc ctggcccttc atgtcgtggg cgatgcgata gagcgcatcg cgcagcggcc
    16861 gtgcgccggg gctcgcctcg agctcgcggc aggcggcatc gagccgctcg gcatcctcgc
    16921 gcgcccattc gaggtagtcg tcgaccaggt cggcgagctt gtcctcggcc ttgcgcagca
    16981 catcggccag gctgtgctcg ctcggcggcg ccttgtcctt gagcgcatgg gaggggcccg
    17041 acatctcgac ctcgcgcgcc tgctcgggcg ccgccggcca gccgagccag tccgggctca
    17101 cgcggccgtc tcccgcggcg acgcggacgc ggcctcgggc tcctcggcgc cgcgctcgat
    17161 cttgcgccgg tccgggccgg catagccgtc gtcgccgtgg cgctggcggt cgggcccgac
    17221 atagtcgggc gtgcgcacga acctgcgcgg cttcaggatc acctcctcga tgcgcatata
    17281 cagcgccttc ggggtcatcg gcttggccag gaactcggtc accccggcat cgcgcgcctc
    17341 ggcgacgcgc gcctcctcgg tatggcccga gatcatgacg atcggcaccg tcgggcacgg
    17401 gctgtcgggg gcgcggcgca gcgtctgcgt gagctcgatg ccgtcgaccg gcgccatcag
    17461 ccagttggtg atcacgatgt cgggggtgaa ggaggccagc acctcgagcg cggaggcgcc
    17521 gtcctcggcc tcgcgaatgg tgcgcgcgcc gaacagcctg agcacgtagc ggatgatcgc
    17581 gcgcatgtgc ctgctgtcgt cgacgaccag gaacgatacc gccgacaggt tgagaatggt
    17641 cctcaccgcg ttactcctgc gggccgcgcc attcttacca tcggtccgca agttacagcc
    17701 acaacttatt gaatctagtg cgcacgtgca gaccggcgag cgccggctga agcacgtcca
    17761 ccgcggcccc atcactaccg gcggttcgag gaaaggaatt tctgattcgc actaatcaga
    17821 ggggtcggag tggcgcgccc aaggggaatc gaacccctgt ttccggcgtg agaggaatcg
    17881 gaaagcgtcc gcgggcgttc gtggccgtcc gacccatcta tttgatatac ggaccttttc
    17941 tcgattctcg ttcgcgcttg tccccggccg tccgcacgcg tatattggac aaatattgga
    18001 cacgttcgcc gctcccgccg cgacggggcg gcatgtcgga gggtaaaagg atggcacgtt
    18061 cggtgacgga caccaagctg agcacccgca cgggccggct ccagctcgcg ccgcgcggca
    18121 agccctacta ccgcgagctc gagcagggct tgcacctggg ctatcgccgc ctgaagcggg
    18181 gcgccggccg gtgggtcgcg cgcgtctatg tcggcaacga gcagtaccgc gccgaggtga
    18241 tcggcaccgc cgacgactac ggcgacgccg cgcccgacga cgaccccgac cgggcgcgca
    18301 tcctggacta caagcaggcg caagaagccg cccggcgcgc ggccgaccgc atccacctcg
    18361 accccggcgc gagcctcgaa ggtgtcggcg ccgagcagcc ggcgggcaac ggccggttca
    18421 cggtcgagcg ggcgctgcgc gactacctca aggcgatgga gcaccggggc cagaacgccg
    18481 ccgattcgcg cgcccgcgcc gag
//
LOCUS       DASWHU010000217        16357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_844829, whole genome shotgun sequence.
ACCESSION   DASWHU010000217 DASWHU010000000
VERSION     DASWHU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16357
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844829"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VGB88_08035"
     CDS             complement(<1..1280)
                     /locus_tag="VGB88_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX9647433.1"
                     /translation="MMARAFAKSAPAASRPAGAACRCAQRSPGPSNRALQAKLTVGPA
                     NDPLERAADRAADRVVAGGPAGALGRVGLGAQRACAACAAEDREIMRRQVAEKEEQEE
                     EGVQAKAEGGGLHAAGAEAAAAAVASGGQPLSTGLRAYFEPRFGHGFSNVRVHDHVRA
                     GVAARAIGARAYTLGRDIAFAPGAWAPASRGGRRLIAHELAHVVQQGSGRETPSATIR
                     RDDGAGPAPGPAPAPAPAPAQPQNYGAPCSGGADDPCQYARCGDRHATILDDLTRAIG
                     YVQDAIRALGTTPLERDTSRALDWYFNDTSSATVSTARERLVCVRDCLQSTWVTNEYG
                     CNPDRTGALAYVCTASPAPCSQDVTPICLTNSHFGNSDRRRAQTMIHECAHRVGMSLG
                     APESVDDIYQHTTRFLFLDTEEALLNSDSYALFAG"
     gene            complement(1277..1525)
                     /locus_tag="VGB88_08040"
     CDS             complement(1277..1525)
                     /locus_tag="VGB88_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647434.1"
                     /translation="MAGEPSRFGAKQSAQVPAAPKIAAAVESRLSETLGAGDAGLPAG
                     GRVDLPRLRLRLPHGSGPEAIAEALSRAVQRAVRGRRP"
     gene            complement(1529..3646)
                     /locus_tag="VGB88_08045"
     CDS             complement(1529..3646)
                     /locus_tag="VGB88_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647435.1"
                     /translation="MTGAARREAEPAPLSADPGWWLARNREVVELGLAWLRGLLEDYI
                     AALRASAPAKVTADGVEVMSDREADWLLRDLAGERRRVAGRATDNAARAAYETARAEL
                     SAAGRESAIDQLSALFGLAPADEDLLLLALAPRAEAAFQALYGYAHDRLSITEATPHL
                     ARALLAPDGGEAWRRLLARLAPDAPLRRFALVQTADGAAALSSFTLDERLARFLVGEA
                     HLDARVRACLRPLAAGDCPLRHRDTVDGLAAAFADAPRPLAQITGPPRSGRRALAAAL
                     AGRFGLALVELDARLVPDAPGERRALFALLAREARIEGLAFLIDADPDRWTGAEERRQ
                     RARQCAEDAAAGLEACVIVVAHDSVALPVAPIRARLGALEAADRSALWHAALGTAAAP
                     LASGIEAVADQFALGPAEIAAVAAGAPPADAAGLWAACREAAGHALDELAERIDPHFT
                     WHDIVLPEGVRDGLVAIAGQVRHRAEVYGRWGFAGRLPRGRGVSALFSGPSGTGKTMA
                     AEVIAGDLDLDLYRIDLSGVVSKYIGETEKNLKRVFDGAEASGAVLFFDEADALFGKR
                     TEVRDSHDRYANIEVSYLLQRMETYGGLAILATNLKSHLDAAFLRRLRYVIDFPLPDA
                     ALRRAIWARAFPPQAPTRELDLDALARLEVAGGNITVIAVNAAFLAAAEARPIGMDHV
                     TRAARAEFRKLDKDFRPLWQGAR"
     gene            complement(3650..4978)
                     /locus_tag="VGB88_08050"
     CDS             complement(3650..4978)
                     /locus_tag="VGB88_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX9647436.1"
                     /translation="MADHTAIAAVSRTLRTLLLDRMVSGAAVTIAPPDVTVAGVDGGR
                     VNLYLYQVHESAALKNQEIPGRGHPAAYGRPPLSLDLRYLVTTHSALETQPDAELNAQ
                     ALLGDAMRVLHHFGNRIDSLAIENPVAGTVGDPILDPALNGEFERVKIVLHPITLDDL
                     TKVWSALSEENFRRSVVYEATVVQIETREPRPAPQPVETRRILATVRRRPEIHALYAT
                     PAPGAPIGELRLRIGDELTIVAEGVLAERLFVRLGTLEPIRVSPPGDGHIRITLPDAQ
                     YAPDLDHPLPRPIAPADFLQPGPLEVQVIVEHPTDGVAGVLDHGVSVSEPRRYRSNVA
                     LVQLVPRITGILPAAGTAADVLQVTGTRLWHPAAGMAEVIVGDAAVAVRAPGAGDPWA
                     APTPTAVEIPVAEAAALLPAPSPAGDPFPVAVQVDGARSRDSGFVFTLGP"
     gene            complement(4988..5791)
                     /locus_tag="VGB88_08055"
     CDS             complement(4988..5791)
                     /locus_tag="VGB88_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647437.1"
                     /translation="MSGFIERLALRAAGLSAADGPALLAPRPHARFETDGGAAPGANG
                     APFEIAAETVASPAQSLAPMEPGLPDAAAPAGTARSDADDAVAPRALPASESPRARPA
                     PSLPRAVSVAVPVPRSARRVVRPDGSAVAQSPPAPQTVVPAPDASRPAPAATAPVHGR
                     APNARPIETSSGPPARAAPVATETAVPSARAKPPLPAPRRAIAEPLPAPGAPPRHRDR
                     APAVTVSIGRIEVEIAPPPAPVAPPSARSQPVRTRGFESYALARRGRVR"
     gene            complement(5788..6648)
                     /locus_tag="VGB88_08060"
     CDS             complement(5788..6648)
                     /locus_tag="VGB88_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647438.1"
                     /translation="MAVETTVTLRHGLGRARTRHREAVVRALTGADEARLAELGPGAS
                     PAARATALIAATTAAIGKVAPVTEDDARGLTAGDRERLILSVYERTFAKPLEAVARCP
                     AEGCGETLEFAFGVDEVLAEPPARAPGDDARTLEVPVEGTVLKVRFRLARGADQEAAA
                     RIAGRDPARAADVVLEGCVLGVEDGAGRAVTPVSVLADLRAPLAEAMARLDPEAETAV
                     RAPCPACGADIVALIDAQSFVDNELSRPGGIFGEVDRLARTYHWAEADILALSVARRR
                     RYLALAAGTA"
     gene            complement(6674..7198)
                     /locus_tag="VGB88_08065"
     CDS             complement(6674..7198)
                     /locus_tag="VGB88_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX9647439.1"
                     /translation="MAQFTVNPRRFDPYKNFKFRVKWDGRYVAGVSKVSALRRTTEVV
                     EHREGGDPSSSRKSPGRSSYEAITLERGVTHDLEFEQWANKVWNFGAGAGSEVSLADF
                     RKDVIIDVFNEAGQKALSYNVYRCWVSEYQSLPEMDANANAVAIQTLKLENEGWERDL
                     AVTEPAEPSFTEPE"
     gene            complement(7219..9258)
                     /locus_tag="VGB88_08070"
     CDS             complement(7219..9258)
                     /locus_tag="VGB88_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX9647440.1"
                     /translation="MPVHPTYPGVYIEEIPSGVRTITSVATSIAAIIGTFRKGPADAA
                     MQMFSMADVERIYGGLERTSAASYAIQQFFLNGGTEAWIVRVANDGSAGDTAAVAAEL
                     VLNTAPDGSGTDVFTARAGRRVRGESVDDPGDWGENLRLEVDYDTSDPATLFNLRVSE
                     VRIDGDRTVVLQTELFRNLTLEVDTPNNALEVVNQGSQLIQLHLDGATPAASDRPATS
                     GTYGADLAATVPALGNIDDLTVTLNLDSGAVSVGPVTLAFDFVAHPPVNFTAWAQELE
                     RAIRAGANDAAFTGDADFQPHHRDYLSGATVRVVGNGAPGNPRHFLIRTGATSRPFDE
                     TATLTFAGADVASYGLDGGAVFGPQLYALDNGDDGTVFDAAGSFVVPATVFQGTRAAK
                     TGLYALEDVDLFNILTLPQVPLMADVDMRAVYAEAEAYAEERRSMIIIDIAPDVIRLE
                     QMQSWLADNESLRHRNGAVYYPRTNVPDPLNRNRPRSIPASGTIAGLWARTDTQRGVW
                     KAPAGTDARLRNVESLAYRLTDQQNGALNPLGINCLRTFPVYANICWGARTLEGADVI
                     ASDWKYVPVRRTTLFIEESLFRGTKWVVFEPNDEPLWAQIRLNVGAFMQDLFRKGAFQ
                     GTTPRDAYFVKCDSETTTQQDIDNGIVNIEVGFAPLKPAEFVILKIQQIARRAEA"
     gene            complement(10200..10277)
                     /locus_tag="VGB88_08075"
     tRNA            complement(10200..10277)
                     /locus_tag="VGB88_08075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10240..10242),aa:Pro,seq:ggg)
     gene            complement(10354..10836)
                     /locus_tag="VGB88_08080"
     CDS             complement(10354..10836)
                     /locus_tag="VGB88_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9647441.1"
                     /translation="MAAGERPQALPERDGEPAAARGAAARKSATAFRTISEVSEALDV
                     PQHVLRFWETKFAQIKPMKRGGGRRYYRPEDIVLLRGIRDLLYVDGYTIKGVQKLLRE
                     SGVRILHERTGTAATGAAETVADADPPAAAGEAAGEEGDLAVVLDQLEALRKLLADVK
                     "
     gene            complement(10839..11159)
                     /locus_tag="VGB88_08085"
     CDS             complement(10839..11159)
                     /locus_tag="VGB88_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX9647442.1"
                     /translation="MATVTRAQLSESVYREVGLSRDESAKLVASVLETIADRLSAGDS
                     VKISSFGSFMVREKAQRVGRNPKTGEEVPILPRRVVVFRPSHVLKDQINDFRARALGA
                     VPGS"
     gene            complement(11377..12441)
                     /gene="plsX"
                     /locus_tag="VGB88_08090"
     CDS             complement(11377..12441)
                     /gene="plsX"
                     /locus_tag="VGB88_08090"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9647443.1"
                     /translation="MTKPITIAIDAMGGDNAPDAIIRGVHLAARRRLETRFIVVGDRA
                     RIEPLLRRYRRILGERATIEHTAEVVAAEDKPSVALRRGRKTSMRLAIDAVADGRAQA
                     VISAGNTGALMAMAKVVLRTLPGIDRPAIAGVVPHRRGEGVFLDLGANIECDAGNLVD
                     FAIMGAVFARAVLGRINPLVGILNVGNEELKGHDEVRQAAAVLREAKDLPLIFHGFVE
                     GDDIAEGTTDVVVTDGFSGNVALKTAEGVSRMYADFVKRALLGSWRGRLGYLIARSAF
                     RAVRQQTDPRHRNGAMFLGVNGTVVKSHGGTDALGFAAAVEVAADAVAEGINQRIIDE
                     LAHLHAAQGSGEPAREAAGS"
     gene            complement(12480..12665)
                     /gene="rpmF"
                     /locus_tag="VGB88_08095"
     CDS             complement(12480..12665)
                     /gene="rpmF"
                     /locus_tag="VGB88_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9647444.1"
                     /translation="MALPKKKKSRARRDMRRSHDSLRRSEPNECPNCGELKLPHHVCP
                     SCGQYRDREILSPAEDF"
     gene            complement(12746..13339)
                     /locus_tag="VGB88_08100"
     CDS             complement(12746..13339)
                     /locus_tag="VGB88_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX9647445.1"
                     /translation="MPKPRASRAAPAPELSRPVTVARVDAKPILLDIAAGEDELAALA
                     ARFGLLALQSLSARAELCRAQARDGSGPAVRVTFDWQAAIVQACVVTLEPVPATLGEQ
                     GVEIEFALDGGEPERRRELSFSLDDPEPPEPPPGPVIDIGEIVAEQLGLAVDPYPRKP
                     DAAFEWAESSGADRAAGRGPFAALGRPRSGGLEEASD"
     gene            complement(13343..13915)
                     /locus_tag="VGB88_08105"
     CDS             complement(13343..13915)
                     /locus_tag="VGB88_08105"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX9647446.1"
                     /translation="MLPWPLRKLVPAARRRERALRAAAVGLYAAAVAQARRPEFYAPA
                     LVPDTVDGRFDLVVLHVVLLVRRLNRDGADGADLAQHLFDTTLDDMDRNLREMGAGDL
                     GVGRRVKAMARAFYGRARAYGAALDAGDRAALETALARNLLGVAGEAHASAAPIAAYV
                     IAAEDRLAALDRVPLWSGSATFPAPPVAGA"
     gene            14053..14505
                     /gene="bamE"
                     /locus_tag="VGB88_08110"
     CDS             14053..14505
                     /gene="bamE"
                     /locus_tag="VGB88_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX9647447.1"
                     /translation="MTRPAQGRRRALRVGGALAAAVAVAACQPRVDTHGFMPNPDLIA
                     QIEPGQQSKSDVAGVLGTPSTVATFDQDTWYYITQKSRTFAFFKPEIVDQEVLVINFD
                     GAEVVSDINRYTIEDGLIIDPVTRKTPTAGKELSFLQQLFGNIGRFSQ"
     gene            complement(14518..>16357)
                     /locus_tag="VGB88_08115"
     CDS             complement(14518..>16357)
                     /locus_tag="VGB88_08115"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9647448.1"
                     /translation="GATLSGAAGFIGMLVSVRANVRTAQASSQSLAAGLEIAFKSGAV
                     TGMLVAGLALLAVAIYFAVMTEGLGFAATERPVIDALVALGFGASLISIFARLGGGIF
                     TKGADVGADLVGKIEAGIPEDDPRNAAVIADNVGDNVGDCAGMAADLFETYAVTVVAT
                     MLLAAIFFTGAEQATMLAFPLVVGGACILASVAGTFLVRLGASGNIMAALYKGLVGTA
                     AMSAVLIAIVIAVWVGFGTVYHAGAREVTGGELFVCALVGLAVTGGLVWITEYYTSTA
                     FRPVRSIASASETGHATNIIRGLAVSMEACALPVLVIAAGIIAAFLAAGLFGLAVAAT
                     TMLALAGMVVALDAYGPVTDNAGGIAEMADLPADVRKATDALDAVGNTTKAVTKGYAI
                     GSAALAALVLFSAYTEDLNYYFPEVQVAFRLQDPYVVIGLFIGGLLPYLFASLGMMAV
                     GRAGGAVVVEVRRQFKEIPGIMEGTARPEYGRAVDLLTRAAIKEMIAPSLLPVLAPVV
                     VYFVISWIGGTAAGFTSLGAMLLGTIVTGLFVAISMTSGGGAWDNAKKYIEDGHHGGK
                     GSEAHKAAVTGDTVGDPYKDTAGPAINPMIKITNIMAILLLAMLAA"
BASE COUNT         2392 a         5740 c         5870 g         2355 t
ORIGIN      
        1 cccgcgaaca gcgcgtagga atcgctgttg agcagcgctt cctcggtatc gaggaacagg
       61 aaccgtgtcg tgtgctggta gatgtcgtcg acgctctccg gcgcgccgag cgacatgccg
      121 acgcggtgcg cgcattcgtg aatcatcgtt tgcgcccgcc ggcggtcgga gttgccgaaa
      181 tgcgagttgg tcaggcagat cggcgtcacg tcctgcgagc aaggcgccgg gctcgcggtg
      241 cagacatagg cgagcgcgcc ggtgcgatcg ggattgcagc catattcgtt ggtcacccat
      301 gtgctttgca ggcagtcgcg gacgcacacc agccgctcgc gcgctgtcga cacggtcgcc
      361 gacgaggtgt cgttgaagta ccagtcgagc gcgcgcgagg tatcgcgctc gagcggcgtc
      421 gtgccaagtg cgcggatggc gtcttgcacg taaccgatcg cgcgcgtcag gtcgtcgagg
      481 atcgttgcgt gccggtcgcc gcagcgcgcg tactggcagg gatcgtcggc gccgcccgag
      541 cacggcgcgc cgtaattctg tggttgggcc ggtgccggtg caggtgccgg cgcagggccg
      601 ggcgcgggac cggcgccatc gtcgcgccgg atagtggcgg acggcgtctc gcggcccgag
      661 ccttgctgca cgacgtgggc gagctcgtgg gcgatcaggc gccggccacc cctgctcgcg
      721 ggcgcccagg cgccgggcgc gaaggcgatg tcgcggccga gggtgtaggc gcgggcgccg
      781 atcgcgcgcg ccgctacgcc tgcgcgcaca tggtcgtgga cgcgcacgtt cgagaagccg
      841 tggccgaagc gcggctcgaa ataggcgcgc aggcccgtcg acaacggctg gccgccgctc
      901 gccaccgccg cggccgcggc ctccgcgccg gcggcgtgca gaccgccccc ttctgccttg
      961 gcctggaccc cctcctcttc ctgctcctct ttctcggcca cctggcggcg cattatctcg
     1021 cgatcctcag ccgcgcacgc cgcgcaggca cgctgcgcgc ccaggcccac gcggccgagc
     1081 gccccggcgg gaccgcccgc gaccacccga tccgccgcgc ggtcggccgc gcgctcgagc
     1141 gggtcattgg ccggccccac cgtgagcttc gcttgcagag cgcggttgct cgggccgggc
     1201 gagcgctgcg cgcagcggca cgccgcgccg gccgggcgcg atgcggccgg cgcgctcttg
     1261 gcgaaagcgc gcgccatcat ggccggcgcc cgcgcacggc acgctgcacg gcgcggctca
     1321 acgcctcggc gatcgcctcc gggccagacc cgtggggcaa ccgcaggcgc agccggggca
     1381 ggtcgacgcg gccccccgcc ggcaaaccgg catcgcccgc gccgagcgtt tcgctgagcc
     1441 gcgactcgac cgccgcggcg atcttcggcg ccgccggtac ctgcgccgac tgcttggccc
     1501 caaaacgcga gggttcgccc gccatcgctt acctcgcacc ctgccagagc ggccggaaat
     1561 ccttgtcgag tttgcggaac tcggcgcggg ccgcgcgcgt gacgtggtcc atgccgatcg
     1621 ggcgcgcctc ggccgcggca aggaacgccg cgttgaccgc gatcacggtg atgttgccgc
     1681 cggcgacctc gagccgcgcc agcgcatcga gatcgagctc gcgggtcggc gcctggggcg
     1741 ggaacgcgcg cgcccagatc gcccggcgca gcgccgcgtc gggcagcggg aagtcgatga
     1801 cgtagcgcag ccgccgcagg aacgccgcat ccaggtggct tttgaggttg gtggcgagaa
     1861 tcgcgagccc gccataggtc tccatgcgct ggagcaggta actcacttcg atgttggcgt
     1921 agcggtcgtg gctgtcgcgg acctctgtgc gcttgccgaa cagcgcgtcg gcctcgtcga
     1981 agaacagcac cgcgcccgac gcctcggcgc cgtcgaacac gcgcttcagg ttcttctcgg
     2041 tctcgccgat atatttcgac accacgcccg acaggtcgat gcggtacagg tcgaggtcga
     2101 ggtcgccggc gatcacctca gcggccatgg tcttgccggt gccgctcgga cccgagaaca
     2161 gcgccgagac cccacggccg cgcggcagcc ggccggcgaa gccccagcgc ccgtagacct
     2221 ctgcccggtg gcggacctgg ccggcgatcg ccaccagacc gtcgcgcaca ccctcgggca
     2281 gcacgatgtc gtgccaggtg aagtgcggat cgatgcgctc ggcgagctcg tcgagggcgt
     2341 gcccggcggc ctcgcggcag gcggcccaca ggccggcggc gtcggcgggc ggcgcgccgg
     2401 ccgcgactgc ggcgatctcc gccgggccga gcgcgaactg gtcggcaacc gcctcgatgc
     2461 cagaggcgag cggcgcggcg gcggtgccga gggccgcgtg ccaaagcgcg ctgcgatccg
     2521 ccgcctcgag cgcgccgagg cgcgcgcgga tcggcgccac cggcaaagcc acgctgtcgt
     2581 gggcgacgac gattacgcat gcctcgagcc ctgcggcggc gtcctcggcg cattggcgcg
     2641 cccgctgtcg gcgttcctcg gccccggtcc agcggtcggg atcggcgtcg atcaggaacg
     2701 ccagcccctc gatccgcgcc tcgcgggcga gcagggcaaa cagcgcgcgg cgctcgcccg
     2761 gcgcgtcagg caccaagcgc gcatcgagtt ccacgagcgc gagtccgaag cgccctgcga
     2821 gcgccgcagc caatgcgcgc cggccgctgc gcggcggccc cgtgatctgg gccaacgggc
     2881 gcggcgcatc cgcgaatgcc gccgccaggc cgtcgacggt atcgcggtgg cggagcgggc
     2941 agtcgcccgc cgccagcggc ctgaggcagg cgcgcacccg cgcatccaga tgcgcttcgc
     3001 cgaccaggaa ccgggccagc cgctcgtcga gagtgaacga gctcaatgcc gcggcgccgt
     3061 cggcagtttg aacgagcgcg aagcggcgca gcggcgcatc gggcgcgagc cgtgcgagca
     3121 gccgccgcca ggcctcgcct ccgtccggcg cgagcagcgc gcgcgcaaga tgcggtgttg
     3181 cctcggtgat cgacaggcga tcgtgggcat agccatagag cgcctgaaag gcggcctcgg
     3241 cgcgcggcgc gagcgcaagc agcaacaggt cctcgtcggc gggggcgaga ccgaacagcg
     3301 ccgagagctg gtcgatcgcc gactcccggc cggcggcgct gagctcggcc cgtgccgtct
     3361 cgtaggccgc gcgcgccgcg ttatcggtgg cccggccagc cacacggcgc cgctcgccgg
     3421 cgaggtcgcg gagcagccag tcggcctcgc gatcgctcat cacctcgacc ccgtcggccg
     3481 ttaccttggc cggcgccgag gcgcgcaagg cggcgatgta gtcctcgagc aatccgcgca
     3541 gccaggcgag gccgagctca accacctcgc gattgcgcgc cagccaccac cccgggtccg
     3601 cgctgagcgg cgccggttcg gcttcgcggc gggcggcgcc ggtcatgggt taggggccca
     3661 aggtgaagac gaagccgctg tcgcggctgc gcgcgccgtc gacctggacc gccaccggaa
     3721 agggatcgcc ggccgggctc ggcgcaggca gcagcgccgc cgcctcggcg accggaatct
     3781 cgaccgcggt cggcgtcggc gcggcccacg ggtcgcccgc gcccggcgcg cgcaccgcca
     3841 ccgccgcatc gccaacgatc acctccgcca tcccggccgc cgggtgccac agccgcgtcc
     3901 cggttacttg cagcacgtcg gcggcggtgc cggcggcggg caagatgccc gtgatccgcg
     3961 gcaccagttg gaccagcgca acgttggagc ggtagcgcct cggctcgctc acggacacgc
     4021 cgtggtcgag gacgccggca acgccgtcgg tcggatgctc gacgatgacc tggacctcga
     4081 gcggccccgg ctgaaggaaa tctgcgggcg cgatcggccg cggcagggga tggtcgaggt
     4141 cgggcgcgta ctgggcgtcg ggcaaggtga tgcggatgtg cccgtccccc ggcgggctga
     4201 cccggatcgg ctcgagcgtg ccgaggcgca cgaacaggcg ctcggcgagc acgccctcgg
     4261 cgacgatggt cagctcgtcg ccgatacgca ggcgcagctc gccgatcggc gcgcccggcg
     4321 ccggtgtcgc gtagagcgcg tgaatctcgg gccggcggcg gactgtggcc aggatgcgcc
     4381 gggtctctac cggctgcggc gccggccgcg gctcacgcgt ctcgatctgg accacggtcg
     4441 cctcgtagac caccgagcgg cggaagttct cttccgacag cgccgaccac accttggtca
     4501 ggtcgtcgag ggtgatcgga tggagcacga tcttgacccg ctcgaactcg ccgttgagcg
     4561 cgggatcgag gatcgggtcg ccgaccgtgc cggcgaccgg gttctcgatc gccagcgagt
     4621 cgatacggtt gccgaagtgg tgcaggacgc gcatcgcatc gccgagcagc gcctgggcgt
     4681 tgagttcggc gtcgggctgg gtctcgagcg cgctgtgggt ggtgaccagg tagcgcaggt
     4741 cgagcgacaa cggcggccgt ccgtaggcgg ccgggtggcc gcggcccggg atttcctggt
     4801 tcttcagcgc cgcgctctcg tggacctggt acaggtagag gttgacccgc ccgccgtcga
     4861 ccccggcgac ggtcacgtcg ggcggggcga tggtcaccgc cgcgccgctg accatgcggt
     4921 cgagcagcaa ggtgcggagc gtccggctga ccgcggcgat ggcggtgtgg tcggccatgc
     4981 cccggtctca ccgaacgcga ccgcgccggg ccaaggcgta agactcgaag ccgcgcgtgc
     5041 gcacgggttg cgaccgggcc gagggcggcg caacgggtgc cggcggcggc gcgatctcga
     5101 cctcgatgcg gccgatcgac acggtcaccg caggtgcgcg gtcgcggtgt cgcggcggcg
     5161 cgccgggcgc cggcagcggc tcggcaatcg cacggcgcgg cgcgggcagc ggcggcttgg
     5221 cccgcgcaga cggcacggcc gtctcggtgg cgacgggcgc ggcacgcgcc ggcgggcccg
     5281 agctagtctc gattggccgc gcgttcggcg cacgcccgtg aacgggcgcc gtcgcggcgg
     5341 gcgccggccg agaagcgtcc ggcgcgggga caaccgtttg cggcgccggc ggcgactgcg
     5401 cgaccgcgga gccatcgggc cgcaccacgc ggcgcgcgga ccgcggcacg ggcaccgcga
     5461 cggaaaccgc gcgcggcagt gacggtgcgg gccgtgcccg cggagactct gacgcgggca
     5521 gcgcccgtgg cgctaccgca tcatcggcgt cggatcgcgc cgtacccgca ggcgccgcgg
     5581 catccggcag acccggttcc atgggcgcca gcgattgagc gggagacgcg acggtctcgg
     5641 ccgcgatctc gaacggcgcg ccgttcgccc cgggcgctgc gccgccgtcg gtctcgaagc
     5701 gcgcatgagg acgcggcgcc agcagagccg gtccgtcggc ggcggacagg ccggcggcgc
     5761 gcaaggcgag tcgttcaatg aatccgctca tgctgtaccg gccgcgagcg cgaggtagcg
     5821 ccgccgtcgc gcgacgctga gcgcaagtat gtcggcctcc gcccaatgat aggtgcgagc
     5881 tagccggtcg acctcgccga agatgccgcc aggtcgggaa agctcgttgt cgacgaagct
     5941 ttgcgcgtcg attagcgcca cgatgtcggc gccgcaggcc gggcacggcg cgcgcactgc
     6001 ggtctcggcc tccgggtcga gcctcgccat agcctcagcc agcggcgcgc gcaggtcggc
     6061 cagcaccgag accggcgtga ccgcccggcc ggcgccgtcc tcgacaccga gcacgcagcc
     6121 ctcgagcacg acgtcggcag cccgtgccgg gtcgcggccg gcgatacgcg cggcggcctc
     6181 ctgatcggcg ccccgcgcaa gccgaaagcg gaccttgagc accgtgccct cgaccggaac
     6241 ttccaaggtc cgggcgtcgt cacccggcgc ccgcgccggc ggctccgcga gtacctcgtc
     6301 gacgccgaac gcaaactcca aggtctcgcc gcagccctcg gccgggcacc gggccacggc
     6361 ctcgagcggc ttggcgaagg tgcgctcata gacgctcaaa atgagccgct cgcgatcgcc
     6421 cgcggtgagc ccgcgcgcgt cgtcctcggt caccggcgcg accttgccga tggcggcagt
     6481 ggtggccgcg atcaacgcgg tcgcgcgcgc cgccgggctc gcgccggggc cgagctccgc
     6541 gagccgcgcc tcgtcggcgc cggtgagcgc gcggaccacg gcctcgcggt ggcgggtccg
     6601 cgcccgcccg agcccgtggc ggagcgtcac tgtcgtctcg acggccatgg cgagccggcg
     6661 cctgcccagc cgctcactcg ggttcggtga agctcggctc cgcgggttcg gtgacggcga
     6721 ggtcgcgctc ccagccctcg ttctcgagct tgagcgtctg gatggcgacg gcattggcgt
     6781 tggcgtccat ctcgggcaac gactggtact ccgacaccca gcagcgatag acgttgtagg
     6841 acaaggcctt ctggcccgcc tcgttgaaca cgtcgatgat cacgtccttg cggaagtcgg
     6901 ccagcgacac ctcggagccg gcaccggcgc cgaagttcca caccttgttc gcccactgct
     6961 cgaactcgag atcgtgggta acgccgcgct cgagcgtgat cgcctcgtag ctgctgcgcc
     7021 cgggcgactt gcggctggac gagggatcgc cgccttcgcg gtgctcgacc acctctgtgg
     7081 tgcggcgcaa cgccgagacc ttgctgacgc cggcgacgta gcgtccgtcc cacttcaccc
     7141 ggaacttgaa gttcttgtac gggtcgaagc ggcgcggatt gactgtgaac tgggccatgg
     7201 cggcgactcc ttacagctct aggcttcggc gcggcgggcg atctgctgga tcttgaggat
     7261 cacgaactcg gcaggcttga gcggcgcgaa gccgacctcg atgttgacga tcccgttgtc
     7321 gatgtcctgc tgggtcgtgg tctcggaatc gcatttgacg aaataggcgt cgcgcggggt
     7381 ggtgccctgg aacgcgccct tgcggaacag gtcctgcatg aacgcgccca cgttgagccg
     7441 gatctgtgcc cacagcggct cgtcgttggg ctcaaacacc acccatttgg tgccgcggaa
     7501 caggctctcc tcgatgaaca gcgtggtccg ccggaccggc acgtacttcc agtccgaggc
     7561 gatcacgtcg gcgccctcga gggtgcgcgc gccccagcag atgttggcgt agaccgggaa
     7621 ggtgcgcagg caattgatgc cgagcggatt gagcgccccg ttctgctggt cggtgagccg
     7681 gtatgccagg ctttcgacgt tgcgcaggcg ggcgtcggtg ccggccggcg ccttccacac
     7741 gccccgctgg gtgtcggtgc gcgcccacag cccggcgatg gtgccgctcg ccgggatcga
     7801 gcgcggccgg ttgcggttga gcgggtcggg cacgttggtg cgcggatagt acaccgcgcc
     7861 attgcggtgg cgcaggctct cgttgtcggc gagccacgac tgcatctgct cgagccggat
     7921 cacgtccggc gcaatgtcga tgatgatcat cgaccgccgc tcctcggcat aggcctcggc
     7981 ctcggcgtag accgcgcgca tgtcgacgtc ggccatcaac ggcacctgcg gcagggtgag
     8041 gatgttgaac aggtcgacgt cctcgagcgc gtacaggccg gtcttcgcgg cacgcgtgcc
     8101 ctggaacacg gtggccggca cgacgaagct gccggcggcg tcgaacacgg tgccgtcgtc
     8161 gccgttgtcg agcgcataga gctgcgggcc gaacacggcg ccgccatcga ggccgtagct
     8221 cgccacgtcg gcgccggcga aggtcagcgt cgctgtctcg tcgaacggac gcgaggtggc
     8281 gccggtgcgg atcaggaagt ggcgcgggtt gccgggggca ccgttgccga ccacgcgcac
     8341 cgtggcgccg gacaggtagt cgcggtggtg cggttggaaa tcggcgtcgc cggtgaacgc
     8401 ggcatcgttg gcccccgccc ggatggcgcg ctcaagctcc tgcgcccagg ccgtgaagtt
     8461 gaccggcgga tgggcgacga aatcgaacgc gagcgtgacc gggccgacgc tgaccgcgcc
     8521 gctgtcgagg ttgagggtca cggtcaggtc gtcgatgttg ccgagcgcgg gcacggtcgc
     8581 ggccaggtcg gcaccgtagg tgccgctggt cgccggccgg tctgaggcgg ccggcgtggc
     8641 gccgtcgaga tggagctgga tcagctgcga gccttggttg accacctcga gcgcgttgtt
     8701 cggcgtgtca acctcgagcg tcaggttgcg gaacagctcg gtctgcagca ccaccgtgcg
     8761 gtcgccgtcg atacgcacct cgctgaccct gaggttgaac agcgtggccg ggtcgctggt
     8821 gtcgtaatcg acctcgagac ggaggttctc gccccagtcg cccggatcgt cgacgctctc
     8881 gccgcgcacg cgacggccgg cgcgggcggt gaacacgtcg gtgccgctgc cgtcgggtgc
     8941 ggtgttgagc accagctccg ccgccaccgc agcggtatcc ccggccgagc cgtcgttggc
     9001 gacgcgcacg atccacgcct cggtgccgcc gttgaggaag aactgctgga tcgcgtagga
     9061 ggcggcgctg gtgcgctcga gcccgccata gatgcgctcg acgtctgcca tgctgaacat
     9121 ctgcatggcg gcgtcggccg gacccttgcg gaacgtgccg atgatcgctg cgatcgaggt
     9181 cgcgaccgac gtgatggtac gaacgccgct tggaatctct tcgatgtaaa cgccgggata
     9241 ggtgggatgg acaggcattc gatttctccc gagcccgtga tgacccaacg gagccgatcg
     9301 gctgcctgct gcgttaagtg gtccgcggcg atcaggcgaa ccgatccgcc ccgcggatgc
     9361 gggacgcccg cgatccgcaa accgagaaga gcgcacgcag tatgactccg cgcggatcgt
     9421 ccctcaatcg caaatgatcc ggggttcgtg gttccgctga cgccgacctg aattcgtctc
     9481 gcatggtcga ggcgaaattt attccaccat gccgatggtg tcggaaggcc gggagcgtaa
     9541 agagtgggcg cttggtcgaa gcgtgctagg cagccgttcg ccaaatccgg tcgccggcaa
     9601 acattggctt tggaacgatt tttcggatca gacggattgc aggttggacc gggcttgtgc
     9661 cgctgcgccg tgcggcctat ctggcgccga atcgcggaca cgtaccgcga gctactatgg
     9721 acggcgcggg ggccgggcga gccggtgata tgatcctcgc ctggacgaag atttggtgcc
     9781 tctaatccac acgaccatga tcgctcaaga tcgtgttagc ctgactctgc gaattgacct
     9841 tgacgcttca cactaaattt gactgcggag gccgatacgt ctcaggcctg tcgtcaacga
     9901 gcgaatagct ttcggatcgg ctgtggtaaa tcacagggtt tccgcccagc tttcggattt
     9961 ccatgaaccg tcgctcggct tgccgcgctc ccgttaagtc cgcattactg cggagcgccg
    10021 cttactcaag ctcactaggt attgcgacac tgcgcttttt ttctggaagt tggcatgagc
    10081 aatctcgcgt ttatgcttcc aatgcggccg atcgcggtgc tgttaccagt actgttacca
    10141 aatcaccaat gccgcttgag agaatgagag cagacttccg actatccctt tgagatgatt
    10201 ggtcggagcg gccggatttg aaccggcgac cccttgtccc ccagacaagt gcgctgacca
    10261 ggctgcgcca cgctccgacc gatcggcggc ccggcgtcgc gaccggaacc gcgggccaac
    10321 atagtgcccg cccgcgcccg ccgcaaccgc ggctcacttg acgtcggcga gcagctttcg
    10381 cagcgcctcg agctggtcga gaacgacggc gaggtccccc tcttcgccgg cagcttcgcc
    10441 ggcggccgcc ggcgggtcgg catctgcgac cgtctcggcc gccccggtag ccgccgtgcc
    10501 ggtgcgctcg tgcaggatgc gcacgccgct ttcgcgcaat agcttctgca cgcccttgat
    10561 ggtgtagccg tcgacgtaga gcaagtcgcg aatgccgcgc agcagcacga tgtcttcggg
    10621 ccggtagtag cgccggccgc cgccgcgctt catcggcttg atctgcgcga acttggtctc
    10681 ccagaagcgg agcacgtgct ggggcacgtc gagcgcctcg ctgacctcgc tgatggtgcg
    10741 gaacgcggtg gcggacttgc gcgccgccgc gccccgcgcc gcggcgggct cgccgtcgcg
    10801 ctcaggcaat gcttgcggcc gttcgcccgc ggccatcatc agctaccggg cacggcgccc
    10861 agggcgcgag cacggaagtc gttgatctgg tccttgagca cgtgcgaggg ccgaaacacc
    10921 accacccgtc gcggcaggat cggcacctcc tcgccggtct tcggattgcg gccaacgcgc
    10981 tgcgccttct cgcgcaccat gaagctgccg aatgacgaga tcttgaccga gtctcccgcc
    11041 gaaagccggt ccgcaatcgt ctccagcacc gaggcaacca gtttcgccga ttcgtcgcgc
    11101 gacagcccga cctcgcggta gacggactcg ctcaactgcg cccgcgtcac cgttgccatc
    11161 gcacgcttcc tcccccacca aagaggccaa gctagctcac ttaagaaaat ccgtcaatat
    11221 caataacatg catgattccc ctgataattc gttgtggccc aacaggcgcc cgcgccgcag
    11281 gaccggaggc gccgggcgcg gcacgccggc tgcccaatcg cgattggtcg gggtcgaagc
    11341 cgggccgagc gcgcgcccgg ggacggccat cggagttcag gaacccgcgg cttcacgcgc
    11401 gggctcaccg gacccttggg cggcgtggag gtgcgcgagc tcgtcgatga tgcgctgatt
    11461 gatgccttcg gcgaccgcgt cggcggcgac ctcgaccgca gcggcaaagc cgagggcgtc
    11521 ggtgccgccg tggctcttga ccacggtgcc gttgacaccc aggaacatcg cgccgttgcg
    11581 gtgtcgcgga tcggtctgct ggcgcacggc gcgaaacgcg ctgcgcgcga tcaggtagcc
    11641 gagccggcct cgccacgagc cgagcagcgc gcgcttgacg aagtccgcgt acatccggct
    11701 gacgccctcc gcggtcttga gcgcgacgtt gcccgagaag ccgtcggtga cgaccacgtc
    11761 ggtggtgcct tcggcgatgt cgtcgccctc gacgaaaccg tggaagatga gcggcaagtc
    11821 cttggcctcg cgcagcacag cggccgcctg gcggacctcg tcgtggccct tcagctcctc
    11881 gttgccgaca ttaagaattc cgaccaatgg gttgatgcga cccaaaactg cacgggcgaa
    11941 caccgcgccc atgatggcga agtccaccag gttgccggcg tcgcactcga tgttggcgcc
    12001 gaggtccagg aacacgcctt cgccgcgccg gtgcggcacc accccggcga tggccggccg
    12061 gtcgatgccc ggcaaggtgc gcaggacgac cttggccatc gccatcagcg cgccggtgtt
    12121 gcccgccgat atcaccgctt gggcgcggcc gtcggcgacg gcgtcgatcg ccaggcgcat
    12181 gctggtcttt cgcccccggc gcagcgcgac cgacggcttg tcctcggccg cgactacctc
    12241 agccgtgtgc tcgatcgtcg cgcgctcgcc caggatgcgg cgatagcgcc tgagcagcgg
    12301 ctcgatccgg gcacggtcgc cgaccacgat gaaccgcgtt tccaggcgcc ggcgcgccgc
    12361 caggtggacg ccacgaatga tcgcatccgg ggcgttgtcg ccgcccatgg cgtcgatcgc
    12421 gatggtgatc ggcttggtca tcgggttaca gccggcgccc cccgagcagc cgaccgcggt
    12481 cagaagtctt cagccgggct cagaatctcg cggtcccggt actggccgca ggacgggcaa
    12541 acgtggtgcg gcagcttcag ctcgccgcag ttcgggcatt cgttgggctc actgcgccgc
    12601 aggctgtcgt gcgaccgccg catgtcgcgc ctggcccgcg acttcttttt cttgggtagc
    12661 gccatcgcct tgcttcctct cagcccggac ggctcgggtt actagaccag aaccgggacc
    12721 ggggcaagtc ccgcgccgcg cccgcctagt cgctcgcctc ttcgaggccg cccgagcgcg
    12781 ggcgcccgag cgcggcgaac gggccgcgtc cggcggccct gtccgcgccc gaggactcgg
    12841 cccactcgaa cgccgcgtcc ggcttgcgcg gatacggatc gacggccaat cctagctgct
    12901 ccgcgacgat ttcgccaatg tcgatgacgg gccccggcgg gggctccggc ggctctgggt
    12961 cgtcgagcga gaacgagagc tccctgcgcc gctcgggctc gccgccgtca agggcgaact
    13021 cgatctcgac cccctgttcc ccaagcgtcg ccggcaccgg ctcgagggtg accacgcaag
    13081 cctggacgat cgcggcctgc cagtcgaagg tgacccggac cgccgggccc gagccgtccc
    13141 tggcctgggc ccggcatagc tcggcccgtg ccgacagcga ttggagcgcg agcaggccga
    13201 accgggcagc gagcgccgcc agctcgtctt cgccggcggc aatgtcgagc aggatcggct
    13261 tggcatcgac cctggcgacg gtcaccggcc gggagagttc cggcgccggg gcggcgcggc
    13321 tggcgcgagg cttgggcacc gatcacgccc cggccaccgg cggtgccgga aacgtcgcgc
    13381 tgccggacca cagcggcacg cggtcgagcg cagcgagccg gtcctcggcc gcgatcacat
    13441 aggcggcgat cggtgcggcc gacgcgtgcg cctcgcccgc gacgccaagc aggttgcgcg
    13501 ccagcgccgt ctcgagcgcc gcccggtcgc cggcatcgag cgcggcgcca taggcgcgcg
    13561 cgcggccgta gaatgcccgc gccatcgcct ttacccggcg gccgacgccg aggtcacctg
    13621 cgcccatttc ccgcaagttc cgatccatgt cgtccaaggt ggtgtcgaac agatgctgcg
    13681 caaggtccgc gccgtcggcg ccgtcgcggt tgagccggcg gaccagtagg accacgtgca
    13741 ggacgaccag gtcgaagcgg ccgtcgaccg tgtccgggac cagggcgggc gcgtagaact
    13801 cgggccggcg cgcctgggcg accgcggccg cgtacagccc aacggcggcg gcgcgcagcg
    13861 cccgttcgcg ccggcgcgcc gcgggcacca gcttgcgcag cggccaaggc agcatcagcc
    13921 gacccgtccg gcgccgcccg ccggcggttg acttggccaa ggcggcgatg acaaattcgc
    13981 ggcctcaaga tcggcgatcg catgagaacg cgcctgctta cacgcggccc ccaagccgcc
    14041 tcggatcggt ccgtgacccg gcctgcgcaa ggtcggcggc gggcgctgcg ggtgggcggg
    14101 gcgctggccg cggcggtcgc ggtggccgcc tgtcagccgc gggtcgacac ccacggcttc
    14161 atgcccaacc ccgacctgat cgcccagatc gagcccggcc agcagagcaa gtccgacgtc
    14221 gccggagtgc tgggcacgcc gtcgacggtg gcgacgttcg accaggacac ctggtactac
    14281 attacccaga agagccgcac cttcgccttc ttcaagcccg agatcgtcga ccaggaggtg
    14341 ctggtgatca atttcgacgg cgccgaggtg gtcagcgaca tcaaccgcta caccatcgag
    14401 gacggcctga tcatcgaccc ggtcacccgc aagaccccga ccgcgggcaa ggagctgtcg
    14461 ttcttgcagc agctgttcgg caatatcggc cggttctcgc aatagcgccg cggcggctca
    14521 cgccgccagc atcgccagca gcaggatcgc catgatgttg gtgatcttga tcatcgggtt
    14581 gatcgcgggc ccggcggtgt ccttgtaagg gtcgccgacc gtatcaccgg tgaccgccgc
    14641 cttgtgcgcc tcggagccct tgccgccgtg gtggccgtcc tcgatgtact tcttggcgtt
    14701 gtcccaggcg ccgccgcccg aggtcatgga gatggcgacg aacaggccgg tgacgatggt
    14761 gccgagcagc atcgcgccga ggctggtgaa gccggccgcg gtgccgccga tccagctgat
    14821 cacgaaatac accaccaccg gcgccagcac cggcaacagc gacggcgcga tcatctcctt
    14881 gatcgccgcg cgggtcagca ggtcgacggc gcggccgtat tcgggccgcg cagtgccctc
    14941 catgatcccg gggatctcct tgaactggcg gcgcacctcg accaccaccg cgccgccggc
    15001 gcgcccgacc gccatcatgc cgagggaggc gaacaggtag ggcagcaggc cgccgatgaa
    15061 caggccgata acgacatagg ggtcctgcag gcggaacgcg acctggacct cggggaagta
    15121 gtagttgagg tcctcggtgt aggcggagaa cagcaccagc gcggccagcg cggcggagcc
    15181 gatcgcgtag cccttggtca ccgccttggt ggtgttgccg accgcatcga gcgcgtcggt
    15241 ggccttgcgc acgtccgcgg gcaggtcggc catctcggcg atgccgccgg cgttgtcggt
    15301 gaccggcccg taggcgtcga gcgccaccac catgccggcc agcgcgagca tggtcgtggc
    15361 ggcgacggcg agcccgaaca ggccggcggc gagaaaggcg gcgatgatgc cggcggcgat
    15421 caccagcacc ggcagggcgc aggcctccat cgataccgcc aggccgcgga tgatgttggt
    15481 cgcgtgcccg gtctcggacg cgctggcgat gctgcgcacg ggacggaatg cggtcgaggt
    15541 gtagtactcg gtgatccaca ccagcccgcc ggtgaccgcg agcccgacca gcgcgcagac
    15601 gaacagctcg ccgccggtga cctcgcgcgc gccggcgtga tagacggtgc cgaagccgac
    15661 ccagaccgcg atcacgatcg cgatcagcac cgccgacatc gccgccgtgc cgacgaggcc
    15721 cttgtagagg gcggccatga tgttgccgct ggcgccgagg cggaccagga acgtgcccgc
    15781 caccgaggcc aggatgcagg cgccgccgac caccagcggg aaggcgagca tggtcgcctg
    15841 ctcggcgccg gtgaagaaga tcgcggccag cagcatggtc gcgaccacgg tcaccgcata
    15901 ggtctcgaac aggtcggcgg ccatgcccgc gcagtcgccg acattgtcgc cgacattgtc
    15961 ggcgatcacc gcggcgttgc gcgggtcgtc ctcggggatg ccggcctcga tcttgccgac
    16021 cagatcggcg ccgacgtcgg cgcccttggt gaagatgccg ccgccgagcc gggcgaagat
    16081 cgagatcagc gacgcgccga agccaagcgc gacgagggcg tcaatgaccg ggcgctcggt
    16141 cgcggcgaag ccgaggccct cggtcatgac cgcgaaatag atcgcgaccg caagaagggc
    16201 gaggccggcg accagcatgc cggtgaccgc gccggacttg aacgcgatct cgaggccggc
    16261 cgccaggctc tggctggagg cctgggcggt gcgcacgttg gcgcgcaccg agaccagcat
    16321 gccgatgaag ccggcagcgc ccgagagcgt ggcgccg
//
LOCUS       DASWHU010000218         6440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1598442, whole genome shotgun sequence.
ACCESSION   DASWHU010000218 DASWHU010000000
VERSION     DASWHU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598442"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGB88_08120"
     CDS             <1..341
                     /locus_tag="VGB88_08120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9647449.1"
                     /translation="VFCGPSAVFTNVLTPRAFVERKDEFRPTLVRRGATVGANATVLC
                     GTTVGAYAMIGAGAVVTRDVADHALVIGSPARRVGWVSRTGERLGSDLVCPRTGERYR
                     ETDGRLEAID"
     gene            334..1464
                     /locus_tag="VGB88_08125"
     CDS             334..1464
                     /locus_tag="VGB88_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX9647450.1"
                     /translation="MTEPFPFVDLAAQQARIRPALDRRIAAVLGHGRYVLGPEVAELE
                     QRLAAFAGAAHAVGVASGTDALQIALMAEGIGAGDAVFVPAFTFPATAEVAVVIGAEP
                     VFVDVDPVTCNIDAADLERRIAETAAEGRLRPRAAIAVDLYGLPADYPALARIADRHG
                     LLLIADAAQSFGASLANRRVGTLAPATATSFFPAKPLGAYGDGGALFTDDGARAEAWR
                     SIRGHGSGGARYDIVRIGLNSRLDTLQAAVLLAKLEVFEDELASRRRIAERYHAALGR
                     ALRCPPMPDGVAQAWAQYTVRVARRDQVAEAMRAEGVPTMVYYPRPLHLQPAYARFAD
                     GAGSRPVSEALAGEVLSLPMHPYLDRARQDRVVAALLAACGR"
     gene            1461..2528
                     /locus_tag="VGB88_08130"
     CDS             1461..2528
                     /locus_tag="VGB88_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9647451.1"
                     /translation="MRRRVAFVLPSLALGGAERIATSLIGALDRTHFEPVLVALTAEG
                     PLAHSLAGDVRVVDLGRARLRGALPALVRGLRGVRADVVVSTLGYLNLALLAVRPLLP
                     AATRIVVREANTPSLALPHAPYALATRLGYRLLYPTADAVLCQHRGTLAEMAERFAVA
                     PARLHELANPVDEDALRAAAATRSAAGPGVRLVAAGRLTRQKGYDRLIPLLAELAPST
                     TLTIFGDGPERGALETLAHQPAVRGRVRLAGTADPLAPELAAADACVLASRWEGLPNV
                     ALEALACGTPVIATPECGGLAALARAGAVTIAPWRAPFRDTLAAVEPTPGGVRPRPSL
                     LPAEFRLAAVTRAFEAVLESL"
     gene            complement(2491..3468)
                     /locus_tag="VGB88_08135"
     CDS             complement(2491..3468)
                     /locus_tag="VGB88_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9647452.1"
                     /translation="MRRLVRRSVALTCAWYLADDARMRWRLARGHIATRSGARHAALP
                     LDESLAYIERIHRDYLAYAGRERFTGTIAEIGPGDNFGLAMLSLCGGAEAVHAIDRYA
                     SVRDAGRQAAIYRALADRHGFHALFDGAPGEAALRGLHLHAGVPAERFFREAGLVFEA
                     ILSRAVMEHLYDPLGALDDMAAALAPGGVMVHRIDFRDHGMFAGHPPLTFLTVPDRLW
                     RAMTRHAGRPNRMLLPAWRDWLARSRLAGSLRITRLAGVDGEIAPAAWADIDATLRAR
                     ALAAVRAVRPRLARSLRPLADQDLAVAGCVLVAERAQLTATRAPPRTRA"
     gene            complement(3483..3926)
                     /locus_tag="VGB88_08140"
     CDS             complement(3483..3926)
                     /locus_tag="VGB88_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9647453.1"
                     /translation="MSEEQAVLAANAAFYAALAAGDYAAMDRLWADAAPVACIHPGWP
                     ILVGREAVMDSWRRILATPPGIACADAACHAIPGPDPGTGTAYVTCMERIGPALLAAT
                     NLFVREQGQWRIAHHQAGHVLETEAAPAPVPGSDPGMAPPHGRLH"
     gene            3959..5083
                     /locus_tag="VGB88_08145"
     CDS             3959..5083
                     /locus_tag="VGB88_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9647454.1"
                     /translation="MTFALPAGRYGPLKSLDGGVLHVITGLHTGGAETQLASLALANH
                     RAGRALAVASLIPGGIHRKRLADAGVPVVDLGMARGRWSWLGPWRLARLIRALRPAAV
                     QGWMYHADLVATAAWLMSGQWRATRLFWGVRCSDLDLGRYGPGLARTVRLCALASRVP
                     CAVVANAETGRAAHRALGYRARAFPVIDNGIDSVRFRPDGEARREVRARYAIAADAPL
                     IAIVARVDPMKDYLTFIQALDRLPGVQALAIGERTESLPEVPRLARLGRRDDVPALLA
                     ACDVIVSSSAFGEGFSNALAEGMACALPAVATDAGDNARLVGGCGRIVPARDPVALAA
                     AVADVLANDPAALGRKARARIVETFSVARMVEAFNRLHDR"
     gene            5229..>6440
                     /gene="asnB"
                     /locus_tag="VGB88_08150"
     CDS             5229..>6440
                     /gene="asnB"
                     /locus_tag="VGB88_08150"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX9647455.1"
                     /translation="MCGIAGVAAPSGLDARAYQAPLARAIERLRPRGPDARGTWHDAC
                     CVLGHTRLKVIDLSPAADQPMAGHGAVICYNGEIYNFAALRAELVGAGIQFRTRSDTE
                     VLLAGWRRWGEALLPRLVGMFAFALWDGEARALVLARDRFGKKPLLYAAEGGRIAFAS
                     DLVALDCLRGERADIDPAALKLLFTLRYLPEPWSIRPGIAKLPPGHLLRLDSAGTKVT
                     PWYRLARQPLPRYANDAEGLVDLRARFDRAVADRLAADVPLGVFLSGGIDSALVAAAA
                     TASGNGAQCFTVGYDGVPGYYDERARARAVARHLGCRHDEIVVPAGAALDALDAVFDG
                     LDEPFGDSSAVPTYLLARAARAHVTVALSGDGADEVFGGYRKYRAERLAGLYRALPAG
                     LRRRVIEPLVEA"
BASE COUNT          718 a         2435 c         2425 g          862 t
ORIGIN      
        1 aggtgttctg cgggccgtcg gcggtgttca ccaacgtgct caccccgcgc gccttcgtcg
       61 agcgcaagga cgagttccgc ccgaccctgg tccgccgcgg cgccaccgtc ggcgccaacg
      121 ccacggtgct gtgcgggacc accgtcggcg cttacgcgat gatcggcgcc ggcgctgtgg
      181 tgacccgcga cgtcgccgac catgcgctcg tgatcggcag cccggcgcgc cgggtggggt
      241 gggtcagccg caccggcgag cggctcggca gcgacctggt gtgcccgcgc accggcgagc
      301 gctatcgcga gaccgacggc aggctcgagg cgattgactg agccgttccc gttcgtcgac
      361 ctggcggcgc agcaggcgcg catccggccc gcgctcgacc ggcgcatcgc cgcggtgctc
      421 ggccacggcc gctacgtgct cgggcccgag gtcgcggaac tcgagcagcg gctcgccgcg
      481 tttgccggtg ccgcgcacgc ggtcggcgtg gcgagcggca ccgacgcgct ccagatcgcg
      541 ctgatggccg aaggcatcgg cgccggcgac gccgtgttcg tgcccgcctt cacctttccc
      601 gccaccgccg aggtggcggt ggtgatcggc gccgagccgg tgttcgtcga cgtcgatccc
      661 gtcacctgca acatagatgc ggccgacctc gagcggcgca tcgccgagac cgcggccgag
      721 gggcggctca ggccgcgggc ggcgatcgcg gtcgatctct acggcctgcc agccgactac
      781 ccggcgctcg cccggatcgc cgaccgccac gggctgctgt tgatcgcgga cgcggcgcag
      841 agcttcggcg cctctctcgc caaccgccgg gtgggcacgt tagcgccggc caccgcgacc
      901 agcttcttcc cggccaagcc gctcggggcc tacggcgacg gcggcgcgct gttcaccgac
      961 gacggcgcgc gcgccgaggc ctggcgctcg atccgcggcc acggcagcgg cggcgcgcgc
     1021 tacgacatcg tccgcatcgg cctcaacagc cggctggata cgctgcaggc cgcggtgctg
     1081 ctggccaagc tcgaggtgtt cgaggacgag ctcgccagcc gccggaggat cgccgagcgc
     1141 taccacgcgg cgctcggccg cgcgctccgt tgcccgccga tgcctgacgg cgtcgcccag
     1201 gcctgggccc agtacacggt gcgcgtcgcc cgccgtgacc aggtcgccga ggcgatgcgg
     1261 gctgagggcg tgccgaccat ggtctactac ccgcgcccgc tgcacctgca gccggcctat
     1321 gcaaggttcg ccgacggcgc gggctcgcgc ccggtgagcg aggcactcgc cggcgaggtg
     1381 ctgagcctgc cgatgcaccc ctatctcgac cgtgcccgcc aggaccgggt ggtcgcggcg
     1441 ctgctcgctg cctgtggccg gtgaggcggc gggtcgcctt cgtgctgccg tcgctcgcgc
     1501 tcggcggcgc cgagcggatc gcgacctcgc tgatcggcgc gctcgaccgg acgcacttcg
     1561 agccggtgct ggtcgcgctc acggccgagg ggccgctcgc gcactcgctc gcgggcgatg
     1621 tcagggtcgt cgatctcggc cgcgcccggc tgcgcggcgc gctgcccgcg ctggtccgcg
     1681 gcctgcgggg cgtgcgcgcc gatgtggtgg tctccaccct cggctacctc aacctggcgc
     1741 tcttggccgt gcgcccgctg ctgccggcgg cgacgcgcat cgtcgtgcgc gaagccaaca
     1801 cgccctcgct cgcgctgccg cacgcgccct atgcgctcgc cacgcggctc ggctaccgcc
     1861 tgctctaccc gaccgccgac gcggtgctgt gtcagcatcg cggcacgttg gccgagatgg
     1921 ccgagcggtt cgcggtcgcg ccggcgcggc tgcacgagct ggcgaacccg gtcgacgagg
     1981 acgcgctgcg cgccgccgcc gcgacgcgca gcgcagccgg ccccggggtg cggctggtcg
     2041 cggccgggcg gctcacccgc cagaagggct acgaccggct gatcccgctg ctcgccgagc
     2101 tcgcgccctc gaccacgctc accatcttcg gcgacgggcc cgagcgcggc gcgctcgaga
     2161 cgctcgcgca ccagcccgcg gtgcggggcc gggtgcggct cgccggcacg gccgacccgc
     2221 tggcgccgga gctcgccgcc gccgatgcct gcgtgctcgc ctcgcgctgg gaggggctgc
     2281 ccaatgtcgc gctcgaggcg ctcgcctgcg gcacgccggt gatcgcgacc cccgaatgcg
     2341 gcggcctggc cgcgctcgcg cgcgcgggcg cggtgaccat tgcgccgtgg cgggcgccat
     2401 tccgcgacac gctggcggcg gtcgagccga cgccaggcgg ggtgcggccg cggccgtcgc
     2461 tgctgccggc cgagttccgc ctggccgcgg tcacgcgcgc gttcgaggcg gtgctcgagt
     2521 cgctgtaagt tgcgccctct ccgccaccag cacgcagccg gcgaccgcga ggtcctggtc
     2581 cgcgagcggc cgcaggctgc gcgcgagccg tgggcggacc gcgcgcacgg cggcgagcgc
     2641 gcgggcgcgg agcgtcgcgt cgatgtcggc ccaggccgcc ggcgcgatct cgccgtcgac
     2701 gccggcgagg cgcgtgatgc gcagcgagcc cgcgagccgc gagcgcgcca gccagtcgcg
     2761 ccacgccggc agcagcatcc ggttgggccg cccggcgtgg cgggtcatcg cccgccacag
     2821 gcggtcgggg acggtgagga aggtgagcgg ggggtggccg gcgaacatgc cgtggtcgcg
     2881 gaagtcgatc cggtgcacca tcacgccgcc gggagcgagc gcggcggcca tgtcgtcgag
     2941 cgcgccgagc gggtcgtaca gatgctccat caccgcccgc gacaggattg cctcgaagac
     3001 caggcccgcc tcgcggaaga agcgctccgc cggcacgcct gcgtggaggt ggaggccgcg
     3061 cagggccgcc tcgcccggcg cgccgtcgaa cagggcgtgg aacccatgac ggtcggcaag
     3121 agctcgatag atcgcggcct ggcggcccgc gtcgcgcacg ctggcgtagc ggtcgatggc
     3181 gtgaaccgcc tcggcgccgc cgcagagact cagcatcgcc aggccgaagt tgtcgccggg
     3241 gccgatctcg gcgatcgtgc cggtgaaacg ctcacgtccc gcataggcca agtaatcgcg
     3301 gtggatgcgc tcgatataag cgagggattc gtcgagcggg agcgcggcgt ggcgcgcgcc
     3361 cgagcgggtg gcgatgtggc cgcgcgcgag ccgccagcgc atccgcgcgt cgtcggcgag
     3421 ataccaggcg caggtcagcg cgacgctgcg ccggaccagc cgccgcacgc cggcgaggcc
     3481 ggtcagtgca gccgcccgtg cggcggcgcc atccccgggt ctgacccggg gaccggcgcc
     3541 ggcgcggcct cggtctccag aacgtggccg gcctggtgat gggcgatccg ccattggccc
     3601 tgctcgcgca cgaacaggtt ggtcgccgcc agcagcgccg ggccgatgcg ctccatgcag
     3661 gtgacatagg cggtcccggt ccccgggtca ggcccgggga tcgcgtggca ggcggcatcg
     3721 gcgcaggcga tgccgggcgg cgtcgccagg atgcgccgcc agctgtccat caccgcctcg
     3781 cgcccgacca ggatcggcca gccgggatgg atgcaggcga ccggcgcggc gtccgcccac
     3841 agccggtcca tggccgcgta gtcgccggcc gcgagcgcgg cgtagaaggc ggcgttggcc
     3901 gccagcaccg cttgctcctc cgacatcgcc cgtctccctc gcccgacccg cgcgcgccat
     3961 gactttcgcc ctgcccgccg gccgttacgg gccgctcaag agcctcgacg gcggcgtgct
     4021 gcacgtcatc accggcctgc acaccggcgg cgccgagacc cagctcgctt cgctcgcgct
     4081 cgccaaccat cgcgccgggc gcgcgctggc ggtggcgagt ctgatccccg gcggcatcca
     4141 tcgcaagcgc ctggcggatg ccggcgtgcc ggtggtcgac ctcggcatgg cgcgcgggcg
     4201 ctggtcgtgg ctcgggccgt ggcgtctcgc ccgcctgatc cgcgcgctca ggcccgccgc
     4261 ggtgcagggc tggatgtacc acgccgacct ggtggcgacc gcggcgtggc tgatgtccgg
     4321 ccaatggcgc gcgacccggc tgttctgggg cgtgcgctgc tcggacctcg acctcggccg
     4381 ctacggcccc gggctcgcgc gcacggtgcg cctgtgcgcg ctcgcctcgc gcgtgccgtg
     4441 cgcggtggtc gccaacgccg agaccgggcg cgccgcgcac cgggcgctcg gctatcgcgc
     4501 gcgcgccttc ccggtgatcg acaacggcat cgacagcgtg cgcttccgtc ccgacggcga
     4561 ggcacgccgc gaggtgcgcg cgcgctacgc gatcgccgcc gatgcgccgc tgatcgcgat
     4621 cgtcgcccgg gtcgatccga tgaaggacta cctgaccttc atccaggcgc tcgaccggct
     4681 gccgggggtg caggcgctgg cgatcggcga gcgcaccgag tcgctgcccg aggtgccgcg
     4741 gctggcgcgc ctcggccggc gcgacgacgt gcccgcgctg ctcgcggcgt gcgacgtgat
     4801 cgtgtcgtcg tcggcgttcg gcgagggctt ctcgaacgcg ctcgccgagg gcatggcgtg
     4861 cgcgctgccc gcagtcgcga ccgacgccgg cgacaatgcg cgcctggtcg gcggctgcgg
     4921 gcgaatcgtg ccggcgcgcg atcccgtcgc gctcgccgcc gcggtcgcgg acgtgctcgc
     4981 gaacgatccc gccgcgctcg ggcggaaagc gcgggcgcgc atcgtcgaga ccttctcggt
     5041 cgcgcgcatg gtcgaggcgt tcaaccgcct gcacgatcgc tagcatgtcg tgctgccgag
     5101 tcgatccgtt cccccctcac ccggctccgg gtaaggcgcg gcattcgccg cgccaaccct
     5161 tcgcaaccct ctccccccac ttgtgggggg agagggaagg gtgagggggg cgctggcggc
     5221 tgagcgcgat gtgcggcatc gccggcgtcg ccgcgccctc ggggctcgat gcgcgcgctt
     5281 atcaagcgcc gctggcgcgc gcgatcgagc gcctgcgccc gcgcggcccg gacgcgcgcg
     5341 gcacctggca cgatgcgtgc tgcgtgctcg gccacacccg gctcaaggtc atcgacctct
     5401 cgcccgccgc cgaccagccg atggccgggc acggcgcggt gatctgttac aacggcgaga
     5461 tctacaactt cgccgcgctg cgcgccgagc tcgtgggcgc gggcatccag ttccgcaccc
     5521 gctcggacac cgaggtgctg ctcgccggtt ggcggcgctg gggcgaggcg ctgcttccgc
     5581 gcctggtcgg catgttcgcg ttcgcgctgt gggacggaga ggcgcgcgcg ctggtgctcg
     5641 cccgcgaccg gttcggcaag aagccgctgc tgtatgccgc cgaaggcggg cgcattgcct
     5701 tcgcctccga cctggtcgcg ctcgactgtt tgcgcggcga gcgcgccgac atcgacccgg
     5761 ccgcgctcaa gctcctgttc acgctccgct atctgcccga gccgtggtcg atccgcccgg
     5821 gtatcgccaa gctgccgccg gggcatctgc tccgcctcga ctcggccggg accaaggtca
     5881 cgccgtggta tcggctggcg cggcaaccgt tgccccgcta tgcgaacgac gccgaggggc
     5941 tcgtggacct gcgcgcgcgc ttcgaccgcg ccgtcgccga tcgcctggcc gccgacgtgc
     6001 cgctcggcgt gttcctgtcg ggggggatcg actcggcgct ggtcgccgcc gcggcaacag
     6061 cctcgggcaa cggcgcgcag tgcttcacgg tcggctatga cggcgtgccc ggctattacg
     6121 acgagcgcgc gcgcgcccgt gccgtcgcgc gccatctcgg ctgccggcac gacgagatcg
     6181 tggtccccgc gggggccgcg ctcgacgcgc tcgacgcggt gttcgacggc ctcgacgagc
     6241 cgttcgggga ttcctcggcg gtgccgacct acctgctcgc ccgcgcggcg cgcgcacacg
     6301 tgacggtggc gctgtcgggc gacggcgccg acgaggtgtt cggcggctac cgcaagtacc
     6361 gcgccgagcg cttggccggc ctgtaccggg cgctgcccgc gggcctgcgc cggcgggtga
     6421 tcgagccgct ggtcgaagcg
//
LOCUS       DASWHU010000219         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3420635, whole genome shotgun sequence.
ACCESSION   DASWHU010000219 DASWHU010000000
VERSION     DASWHU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420635"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..862
                     /locus_tag="VGB88_08155"
     CDS             455..862
                     /locus_tag="VGB88_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX9647456.1"
                     /translation="MDGPANAARRFLGGRCGRGGAAAAEFAIILPVLLAVVMGIVEFA
                     WMYHVENEMAYVARGVTRDVSVGTYTTAQATAEVTDRLSHFGYPFTVNVNEVANDVTI
                     EVSVPMASAALLNIMGIFSAGSIDTSVTMRKEP"
     gene            complement(902..2305)
                     /locus_tag="VGB88_08160"
     CDS             complement(902..2305)
                     /locus_tag="VGB88_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9647457.1"
                     /translation="MRATDPAFLSASAAAEAIGAGRLSPVDLVEALLARIDAHEAKLH
                     AFVAVYGDDARLAAEAAHKAIRAGHAVGPWHGVPIALKDIVDLEGRVTMGGSKHWAGR
                     VSKLTATLARRLIAAGMIVIGKTHTVEFAMGGWGTNRQLGTPWNPWDLYTHRTPGGSS
                     SGSGVATAARLAPWAIGTDTGGSVRLPSSWCGLTGLKTTIGRISVHGVLPLAPTMDTP
                     GPMARTVEDAARLFNLLQGPDPDDPLTLRHPPDDPLPALRRGVAGLRLGVLAEAERAG
                     IDGEVLAAYDAAAETLAGLGARIDPLSLPRSSEDYGAMVGKLIGAEGYSLVGELVDDA
                     SLPLDDDVRPRIQVGRDISATAYLRLLREREACKRAALTAIEGFDAWLTPTTATPALA
                     LDAVDQTTTPARFTRPVNLLDWCALALPNGATGGGLPISLQVVCRGHDEATALRIGWA
                     YQQATDWHNRLPPGLDR"
     gene            2431..3186
                     /locus_tag="VGB88_08165"
     CDS             2431..3186
                     /locus_tag="VGB88_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgiC"
                     /protein_id="HEX9647458.1"
                     /translation="MGDYLAWRAKFGVVTPSTNTVVQPEYDDMRPAGVTNHLARIHIP
                     DMRIESNEDFESLIARIDADLDNAIDRVMTCKPDHLILGISALSVWGGSVAAGAALKE
                     RMHRRAGAAIGVSHAAEAVPAALRAYGAGRRIAVVEPYYPTVEPRLGGVFRELGYDIV
                     RYRHLKGRSPVTYTHLGARDMIEALKGIDGDDVEAIVQFGANLPMARIAGEAERWLGK
                     PVVSVNVATYWHALRACGITDRIEGFTRLLTEF"
     gene            complement(3212..3285)
                     /locus_tag="VGB88_08170"
     tRNA            complement(3212..3285)
                     /locus_tag="VGB88_08170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3251..3253),aa:Gln,seq:ctg)
     gene            3551..4600
                     /locus_tag="VGB88_08175"
     CDS             3551..4600
                     /locus_tag="VGB88_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9647459.1"
                     /translation="MAGADGSPAGGAGDAASAPIFAALDLGSNNCRLLIARPCSDGFR
                     VIDAFSRIVRLGEGMAGPDSQLVLSEPAMRRTVKALRVCANKMRRRAVASARCVATEA
                     CRRAANSRGFVKRVRRQTGIALEVISNRQEAELAVAGCAPLFDPEAENILIFDIGGGS
                     TEIIWVALGAGARPRILDAWSLPCGVITFAERFGSDRFSPDTYRAMVDHARCLIAPFE
                     TAHAIAAAVRGGRVQMVGTSGTVTTLAGIELDLARYDRARVDGSYLDVATMRRLSRRL
                     ADLDCDARAAHACIGRERADLVVGGCAVVEAICDAWPVRRLRVADRGVREGILYDLIA
                     ADDAARAAQAAAGDA"
     gene            4619..5380
                     /locus_tag="VGB88_08180"
     CDS             4619..5380
                     /locus_tag="VGB88_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX9647460.1"
                     /translation="MAGEGPGRGGKRRRGAAARTPTVRVKTAKGRKTSSTRWLQRQLN
                     DPYVVRAKAEGYRSRAAYKLIEIDDRFRILAKGRRVLDLGAAPGGWTQVAVARVGAGK
                     PGGGAVVALDSVPMRPIAGATLLRLDFLGEAAPAALAEAAGGPIDVVLSDLAPAATGV
                     ARIDHLRIMALCEAVIDFAMGVLAPGGALVTKVWRGGGEAALVARLKRAFATVRHVKP
                     PASRPESAELYLVAIGFRGAGPEAGGTPPPRPARA"
     gene            complement(5404..>5584)
                     /locus_tag="VGB88_08185"
     CDS             complement(5404..>5584)
                     /locus_tag="VGB88_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647461.1"
                     /translation="AVGTPGRRKVARADWIEESARRILIDAAYGREIEWLADMGNDSA
                     KSAIEAAHRIDSAKR"
BASE COUNT          751 a         1970 c         2087 g          776 t
ORIGIN      
        1 atgccccgcg tcgacatcgg cactgccgcg ctttacgacg gcgataacgc cttgaacggc
       61 cgcgcggcac cccgggtggc gttgctcggg gggcacggcg ggaggcgtcg tccgatggcg
      121 aagcgaaacg atcgagcgag cacgcgcaac ccgtcgccgt cgccggaccg gcccttggca
      181 cctggcagcc gactcgcggt cgccgtcgtt ttggcatagc atcggtttgg aggagcggcg
      241 accgaacgga gactcacagg tctgccgaag gggtgcatgt cgcgagccgc gcgcgaccat
      301 gtgtcgcgcc ggcgacgccg gaggacggcg atccgcgagg tgcatcgaac agggcgattt
      361 gagaccggcg gagctgacgc gaatcatgtc ggaagcatgg ccgaaccggc aggccgaggc
      421 cgcttgccgg aaacagggag ccgggcgagc cgggttggac gggccggcga atgctgcgcg
      481 ccggttcctc ggcggcaggt gtggacgggg cggtgccgcc gcggccgagt tcgcgatcat
      541 cctgccggtg ctgctggcag tggtgatggg aatcgtcgag ttcgcgtgga tgtatcacgt
      601 cgagaatgag atggcgtatg tcgcgcgcgg ggtcacgcgc gacgtttcgg tgggtaccta
      661 caccaccgcc caggcgaccg ccgaggtgac cgatcgcctg agccatttcg ggtacccgtt
      721 caccgtcaac gtcaacgagg ttgccaatga cgtcaccatc gaggtctcgg tgccgatggc
      781 gagcgccgcg ctgctcaaca tcatgggcat cttcagcgcc ggatcgatcg atacctctgt
      841 gaccatgcgc aaggaaccgt gaccgggcgc gcgcccgccc ttggcgggga cgcgcgccgg
      901 gtcagcggtc gagccccggc ggcagccggt tgtgccagtc ggtcgcctgc tgatacgccc
      961 agccgatgcg caatgccgtc gcctcgtcat ggccgcggca gacgacctgc agcgagatcg
     1021 gcaagccgcc gcccgtcgcc ccgttcggca gcgcaagcgc gcaccagtcg agcaggttca
     1081 ccggccgggt gaagcgcgcc ggtgtcgtcg tctggtcgac cgcatccagc gccagcgccg
     1141 gcgtcgcggt ggtcggcgtg agccaggcgt cgaacccctc gatcgcggtg agcgcggcgc
     1201 gcttgcacgc ctcgcgctcg cgcagcagcc gcaggtaggc ggtggccgag atgtcgcggc
     1261 cgacctgaat gcgcgggcga acgtcgtcgt cgagcggcag gctcgcatcg tccaccagct
     1321 cgcccaccaa cgagtacccc tcggcgccga tcagcttgcc gaccatggcg ccgtagtcct
     1381 cgctcgagcg tggcagcgac agaggatcga tgcgcgcgcc gagcccggcg agcgtctcgg
     1441 ccgccgcgtc gtaggcggcg aggacctcgc catcgatgcc ggcgcgctcg gcctcggcga
     1501 gcacgccgag acgcaggccg gcgacgccgc gacgcagcgc cggcagcggg tcgtcgggcg
     1561 gatgacgcaa ggtcagcggg tcgtcggggt ccggcccctg gagcaggttg aacagccggg
     1621 cggcgtcctc gaccgtgcgc gccatcggcc ccggggtgtc catggtcggc gccagcggca
     1681 gcacgccgtg cacgctgatc cggccgatgg tcgtcttgag cccggtgagt ccgcaccagg
     1741 acgacggcag gcgtaccgag ccgccggtat cggtgccgat cgcccacggt gcgagcctgg
     1801 cggcggtggc gacacccgag ccgctgctgg agccgcccgg ggtgcggtgc gtgtagaggt
     1861 cccaggggtt ccacggcgtg ccgagctggc ggttggtgcc ccaaccgccc atcgcgaact
     1921 cgaccgtgtg ggtcttgccg atgacgatca tgccggccgc gatcaggcgc cgcgccaggg
     1981 tggcggtgag cttggagacg cgccccgccc aatgcttgct gccgcccatg gtgacgcggc
     2041 cctcgaggtc gacgatgtcc ttgagcgcga tcggcacgcc gtgccagggg ccgaccgcgt
     2101 ggccggcgcg gatcgccttg tgcgccgcct cggcggcgag cctggcgtcg tccccgtaca
     2161 ccgcgacgaa ggcgtgcagc ttcgcctcgt gcgcatcgat gcgggcgagc agcgcctcga
     2221 ccagatcgac cgggctcagc cgccccgcgc cgatcgcctc ggccgcggcg gacgcgctca
     2281 ggaacgcggg atccgttgcc cgcatgtttg acccccctta cggtatcggc gaaatctgtc
     2341 atgcggcccg cggccgggca agagcggcgc cgcgccggcg cgccgcgttg cccgggggcg
     2401 cggttccggg actcacggga ggcgacttga atgggcgact atctcgcctg gcgcgcgaag
     2461 ttcggcgtgg tgacaccctc gaccaacacc gtcgtccagc ccgagtacga cgacatgcgc
     2521 ccggcgggcg tgaccaacca cctcgcgcgc atccacatcc cggacatgag gatcgagtcc
     2581 aacgaggact tcgagtcgtt gatcgcccgg atcgatgccg acctcgacaa cgccatcgac
     2641 cgggtgatga cctgcaagcc ggaccatctg atcctgggca tctcggcgct cagcgtctgg
     2701 ggcggcagcg tcgcggcggg cgcggcgctg aaagagcgga tgcaccgccg cgccggcgcc
     2761 gcgatcggcg tgagccacgc ggccgaggcg gtgcccgcgg cgctccgggc ctatggcgcc
     2821 gggcgccgga tcgcggtcgt ggagccctat tacccgacgg tcgagccccg cctcggcggc
     2881 gtgttccggg agctcggcta cgacatcgtc cgctaccgcc acctcaaggg ccgcagcccg
     2941 gtgacctata cccatctcgg cgcgcgcgac atgatcgagg cgctgaaggg gatcgacggc
     3001 gacgacgtcg aggcgatcgt ccagttcggc gccaacctgc cgatggcgcg catcgccggc
     3061 gaggccgagc ggtggctggg caagccggtg gtcagcgtca acgtcgccac ctactggcac
     3121 gccctgcgcg cctgcggcat caccgaccgg atcgagggat ttacccgcct gctgacggag
     3181 ttctaggcga gtggcgcgcg cggcggcgga atggctgggg gaccaggatt cgaacctgga
     3241 ctgctcggtc cagagccgag agttctaccg ttgaactatc ccccaagccg gggccgccaa
     3301 gcggggcgtc gcggggcaag gtaggaaccg cccggcgccg agtcaagcaa accggccgga
     3361 acgtgcggca cggcgcgcgc cccgaccgga cccggtgcgg cgcgggtgtt ccccaagtgc
     3421 agcgggtcgg cgcggtgtgc taccatcgcc caggcgagga gcggtgacac cgcgctgttc
     3481 cgcagccctt cgggcggcgg cagggcaacg gcgggcgggt ggtgctgagg tgtcgaatgt
     3541 cggacaggct ttggccgggg cggacgggtc gcccgccggc ggcgcgggcg acgcggcttc
     3601 ggcgccgatc ttcgccgcgc tcgacctcgg ctccaacaac tgccgcttat tgatcgccag
     3661 gccgtgcagc gacggcttcc gggtgatcga cgccttctcg cgcatcgtcc gcctcggcga
     3721 gggcatggcc gggcccgaca gccagctggt cctgtccgag ccggcgatgc ggcgcacggt
     3781 caaggcgctg cgcgtgtgcg cgaacaagat gcgccggcgc gccgtcgcca gcgcgcgctg
     3841 cgtcgccacc gaggcctgcc ggcgcgccgc caattcgcgc ggcttcgtca agcgcgtccg
     3901 ccgccagacc gggatcgcgc tcgaggtgat ctcgaaccgg caggaggcgg agctcgcggt
     3961 cgccggctgc gcgccgctgt tcgacccgga ggccgagaac atcctgatct tcgacatcgg
     4021 cggcggctcg accgagatca tctgggtggc gcttggtgcc ggggctcgcc cgcgcatcct
     4081 cgatgcctgg tcgctgccgt gcggcgtgat caccttcgcc gagcggttcg gcagcgaccg
     4141 tttttcaccc gacacctatc gcgccatggt cgatcacgcc cgctgcctga tcgcgccgtt
     4201 cgagaccgcg cacgcgatcg cggccgcggt ccgcggcggc cgggtgcaga tggtcggtac
     4261 ctcgggcacg gtcaccacgc tcgccggtat cgagctcgac ctcgcgcgct acgaccgcgc
     4321 gcgcgtcgac ggctcgtacc tggacgtcgc caccatgcgc cggttgtcgc gccggctcgc
     4381 cgatctcgat tgcgacgcgc gcgcggcgca tgcgtgcatc gggcgcgagc gcgccgacct
     4441 ggtggtcggc ggctgcgccg tggtcgaggc gatctgcgac gcctggccgg tgcgccggct
     4501 gcgggtcgcc gatcgcggcg tgcgcgaggg catcctgtac gacctgatcg cggccgacga
     4561 cgcggcgcgc gccgcccagg cggccgcggg cgacgcctga gcgggcgcgc cgccgctcat
     4621 ggccggcgag gggccgggac gcggcggcaa gcggcggcgc ggcgcggcgg cgcgaacgcc
     4681 cacggtgcgg gtcaagaccg ccaagggacg caagacctcg tcgacccgct ggctgcagcg
     4741 ccagctcaat gacccctatg tcgtgcgcgc caaggccgag ggctatcgca gccgcgccgc
     4801 ctacaagctg atcgagatcg acgaccgttt ccgcatcctg gccaagggcc ggcgcgtgct
     4861 cgacctcggc gccgcgcccg gcggctggac ccaggtggcg gtggcgcggg tcggcgcagg
     4921 caagcccggc ggcggcgcgg tggtcgccct cgacagcgtg ccgatgaggc cgatcgccgg
     4981 agccacgctt ctgcgcctcg acttcctcgg cgaggctgcg ccggcggcgc tcgccgaagc
     5041 cgcgggcggg ccgatcgacg tggtgctcag cgacctcgcg ccggccgcca ccggcgtcgc
     5101 ccgcatcgac cacctgcgga tcatggcctt gtgcgaggcg gtgatcgatt tcgccatggg
     5161 cgtgttggcg ccgggcgggg cgctggtcac gaaagtgtgg cgcggcggcg gcgaggcagc
     5221 cctggtggcc cgcctcaagc gcgccttcgc caccgtccgg cacgtcaagc ctccggcgag
     5281 ccggcccgag tcggcggagc tttacctggt agcgatcggc tttcgcggtg ccggacccga
     5341 ggccggcggc acgccgccgc cgcggccggc gcgcgcctga tcaagatggg tttgcggtcg
     5401 ggctcagcgc ttggccgagt cgatccgatg cgcggcctcg atcgcgctct tggcggagtc
     5461 gttgcccatg tcggcgagcc attcgatctc gcgcccgtag gcggcgtcga tcaggattct
     5521 gcgcgcgctc tcctcgatcc agtcagcgcg cgcgaccttg cggcggcccg gggtgccgac
     5581 ggcg
//
LOCUS       DASWHU010000220         8534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1212792, whole genome shotgun sequence.
ACCESSION   DASWHU010000220 DASWHU010000000
VERSION     DASWHU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212792"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGB88_08190"
     CDS             <1..376
                     /locus_tag="VGB88_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX9647462.1"
                     /translation="AEALLAHRFAVINVWRPIRGPVKCAPLAVCDAQSIAPGDLVATD
                     LVYRDRTGEVYQARFNPGHRWYYFPEMRCDEAMLIKCYDSATDGRARFTAHTSFDDPT
                     SAADAPPRESIEARTLAFFADD"
     gene            complement(403..618)
                     /locus_tag="VGB88_08195"
     CDS             complement(403..618)
                     /locus_tag="VGB88_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX9647463.1"
                     /translation="MPDHVYAVTEIVGSSSKSIDDAIRNAVRTAAQTLRNLHWFEVVE
                     TRGMIDGTEVGYFQVTVKIGFRHEDKG"
     gene            844..2385
                     /locus_tag="VGB88_08200"
     CDS             844..2385
                     /locus_tag="VGB88_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX9647464.1"
                     /translation="MTDTPPRVPGPGGDSPATRANAFGLGLGALAFVAIELSPAPEGL
                     SPAGWHCAAIAALIAVWWVTEAIPLSATALLPLLLFPVLGVASIREAAVPYANPVIFL
                     MLGGFILALGMQRWNLHRRIALFLVSQAGTRPANVIGGFMLASALISMWVFNTTTTVM
                     MLPIALSVIEFVQRNTESDPSHARAGRQFATALMIAIAYAATIGGMGTLIGTAPNAIL
                     AGFMSETYGVTVSFLDWMLVGVPMIALMLPLAWLVLTRIAFPVRLPEVAGQAQLIRDE
                     LRALGPMSRGERTVAAVVGVTAFLWVFRPLIEDAVPGISLNDTSIAFFGALALFVIPV
                     DARKGIFALSGEWARNLPWGVVILFGGGLSLADGIKSSGLADWIGAGTGGLATLPTVA
                     VVLAVVVLIVMLTELTSNTATTATFLPIVAAIAIGIGENPFLLVFPTALAASCAFMMP
                     VATPPNAVVFASGHVTISQMIRSGIMVNIVGVVVTMLIAYVAVRAVFGIELGVLPDWA
                     ANAGK"
     gene            complement(2397..2873)
                     /locus_tag="VGB88_08205"
     CDS             complement(2397..2873)
                     /locus_tag="VGB88_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647465.1"
                     /translation="MINRLLIACLALAAVPAAAEPREWLGFSAVERTIEAEIKVADGA
                     TVIRRRSSGLYFSSDPSLVFNKSSFECTGSFYVPADDPEGRGYGQCLGIDPQGNAWWM
                     AYDGARNSGAWHFLGGTGRYDGIAGSGTWTAIPSTGPRRFVTTWDGEWSVERPPSQ"
     gene            3037..3555
                     /locus_tag="VGB88_08210"
     CDS             3037..3555
                     /locus_tag="VGB88_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9647466.1"
                     /translation="MASRRILAWISAAAVGGWAALWVAALSPAATVPAPSGPIACAGC
                     DLAGVDWAHRALEGAVLDGADLSGARLTGARMAGARLAGARLAGANLFGADLTGADLT
                     GASLAEANLASGVLAGADLAGADLSRANLVGTVLTGARLDGARLAGAFIHDAYLCGVR
                     LPAAAGTHHRRC"
     gene            complement(3563..3985)
                     /locus_tag="VGB88_08215"
     CDS             complement(3563..3985)
                     /locus_tag="VGB88_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647467.1"
                     /translation="MAHARILAAALLAAMASPAAFADEAGDGRALYETECAYCHFSAE
                     LPLAGRRDPGARLVPVMMATYGPRLAGIVGRAAGADPAFNYSESFVAETRDLVWTAPN
                     LDRLMTDARAMIPGVRMFYRQPDANIRRKILAYLETTG"
     gene            complement(3995..4435)
                     /locus_tag="VGB88_08220"
     CDS             complement(3995..4435)
                     /locus_tag="VGB88_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX9647468.1"
                     /translation="MAQMLVAAVSGIVFGLGLGVSQMVDPAKVLAFLDLAGDWDPSLA
                     FVMGGAVAVSLVAFRYSLRRRAPLLARSFALPEVSGIDARLLAGAALFGAGWGLVGFC
                     PGPAIASFAFGQVESALFVAAMAAGMAVANLVPGPSRRLGTTEA"
     gene            complement(4435..4866)
                     /locus_tag="VGB88_08225"
     CDS             complement(4435..4866)
                     /locus_tag="VGB88_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9647469.1"
                     /translation="MENFTPVSALIGGVLIGASAALLMLMNGRLAGVSSIVAGVVPPG
                     SVEQAAWRGLFLAGLVAGALIHRTVIDPGASITIAAPLPALLIGGLLVGVGTRIGAGC
                     TSGHGVCGLARLSPRSLVATLTFMAVAMLVVFAVRHVAGGW"
     gene            5156..5758
                     /locus_tag="VGB88_08230"
     CDS             5156..5758
                     /locus_tag="VGB88_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9647470.1"
                     /translation="MLLIENLRSLRADVAPVSFTLDDGACLAVHGPSGAGKTLLLRAI
                     ADLDPNEGAVTLDGTPRESLRAPHWRRLTVYVPTESGWWEDTVGHHFEDPEAVRPLLD
                     DLGLPADIRQWPVARLSTGERQRLALARALVLKPRVLLLDEPTAALDPEAAAAAEAAI
                     ARLLAVGASALWVSHDLAQRRRVAARSLAIPDGRIEDRAR"
     gene            5755..6558
                     /locus_tag="VGB88_08235"
     CDS             5755..6558
                     /locus_tag="VGB88_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647471.1"
                     /translation="MTYIELAYTDLAIASLLVVLNALLSLVLALRMQQQLLVAGARMI
                     VQLALVAVVLKVLFAHVSPLWTGLAAATMILFAGREIVARQERRLAGLWAYGLGTGCM
                     LLAACLVTVFALTTQIRPDPWYDPRYALPLLGMVLGNTMTGISLGLHTLTTGLARDRV
                     AVEARLALGGSFWQAALPTTRSAMRSGLMPIVNAMAATGLVSLPGMMTGQILAGADPV
                     QAVKYQVLIMFLIAGGTGIGVLLAVAGGLARLTDARQRLRLDRLGPARG"
     gene            6686..6886
                     /locus_tag="VGB88_08240"
     CDS             6686..6886
                     /locus_tag="VGB88_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647472.1"
                     /translation="MRAVSTARSLAESDNEMIRVIEDLVTLLIDKKVIRSGELPVAAL
                     EKVIKRRLWRHRMNRRPAEPLE"
     gene            complement(6950..8530)
                     /locus_tag="VGB88_08245"
     CDS             complement(6950..8530)
                     /locus_tag="VGB88_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9647473.1"
                     /translation="MALSAVAGESWAAAQSPPPAWVPPSSVQPPTIRQLEHVARQFHL
                     HLTTEDLEEYRSLMAGILGSYRRLDLYAEPRLPVKYPRDPGYRPPASENPLNAWYWRC
                     TIKGAASGKLAGKKIAIKDNVCVAGVPMMNGASVLEGFVPDQDATIVTRILDAGGEIV
                     GKAVCEHFCFSGSSHTSDTGYVLNPHDATRSAGGSSSGSAALVVSGAVDMAIGGDQGG
                     SIRIPSSYSGAFGLKATYGLVPYTGVFPIELTLDHTGPIAGSAADCALLLEVLAGPDG
                     LDPRQARGWQGQDYTKALTGDANGLKIGIVAEGFGWEGLSEPDVDRIVRESAMRLGDA
                     GAEVREISVPMHRDGVHVWNAIAVEGAHMLMIDGNSMGTNWQGHYLTQLLDFYGRSRR
                     VRADDYSETCKLVLLLGEYMQQAYHGRYYAKAQNLARVLREAYDDAFAGVDLLVMPTT
                     PQKATVLPPAGASKTEIVAHALNMIHNTCPFDVTGNPAINVPCAMSEGLPVGMMLIGR
                     LGEDATVLRAAHAYQQLG"
BASE COUNT         1189 a         3015 c         3048 g         1282 t
ORIGIN      
        1 ggccgaggcg ctgctggcgc accgcttcgc ggtcatcaac gtctggcggc cgatccgcgg
       61 gccggtgaag tgcgcgccgc tcgcggtctg cgatgcccaa agcatcgcgc ccggcgacct
      121 ggtcgcgacc gacctcgtct accgcgaccg caccggcgag gtctatcagg cgaggttcaa
      181 cccgggccac cgctggtact atttccccga gatgcgctgt gacgaggcga tgctgatcaa
      241 gtgctacgac tcggccaccg acggccgcgc ccggttcacc gcgcacacct cgttcgacga
      301 cccgacctcg gccgcggacg ccccgccgcg cgagagcatc gaggcccgca cgctcgcctt
      361 cttcgccgac gattagccgc gtcgtcgccg cgcgcccggc ggctacccct tgtcctcgtg
      421 gcggaagccg atcttgaccg tcacctggaa gtagccgacc tcggtgccgt cgatcatgcc
      481 gcgcgtctcg acgacctcaa accaatgcaa attgcgcaag gtctgggcgg cggtgcgaac
      541 ggcgttgcgg atggcgtcgt cgatgctctt gctcgacgag ccgacgatct cggtgaccgc
      601 gtagacgtga tccggcatgc ctgcctcctg tcgcacgttc aagacctgtg gcgacgacgt
      661 cttgggcgtc gtctcgcgac tatatacgtg cgtcaggcgc cgatcacggc acaatcgcac
      721 ctgacggcgg cgcggcgttg aacgcgcgcc ggtaaatccc taccatcggg ccggccggat
      781 cgcgtgcggg ggggcaagca tgactgtggc gacgccgacg gttgcgtctc ggggaagccg
      841 gccatgaccg acacgccgcc gcgcgtgccg gggccgggag gcgactcccc ggcgacccgc
      901 gccaacgcgt tcggcctcgg cctcggcgcc ctcgcgttcg tcgcgatcga gctctcgccc
      961 gcgcctgaag ggctgtcgcc ggcaggctgg cattgcgcgg cgatcgcggc tctgatcgcc
     1021 gtgtggtggg tgaccgaggc gatcccgctg tcggcaaccg cgctgctgcc gctgctcctg
     1081 ttcccggtgc tcggcgtcgc ctcgatccgc gaggccgcgg tcccctacgc caacccggtc
     1141 atcttcctga tgctcggcgg cttcatcctg gcgctcggca tgcagcgctg gaacctgcac
     1201 cggcggatcg cgctgttcct ggtgtcgcag gcgggcacgc gcccggcgaa cgtgatcggc
     1261 ggcttcatgc tcgccagcgc gctgatcagc atgtgggtgt tcaacaccac gaccacggtg
     1321 atgatgctgc cgatcgcgct gtcggtgatc gagttcgtcc agcgcaacac cgagtccgat
     1381 ccttcgcacg cgcgggccgg gcgccagttc gcgaccgcgc tgatgatcgc catcgcctat
     1441 gccgccacca tcggcggcat gggcaccctg atcggcaccg cgcccaacgc gatcctcgcc
     1501 ggcttcatgt ccgagaccta cggcgtgacg gtgagcttcc tcgactggat gctggtcggc
     1561 gtgccgatga tcgcgctgat gctgccgctc gcctggctgg tgctgacccg gatcgcgttt
     1621 cccgtgcgcc tgcccgaggt ggcggggcag gctcagctga tcagggacga gctcagggcg
     1681 ctgggcccga tgtcgcgcgg cgagcgcacg gtggcggcgg tggtcggcgt gaccgcgttc
     1741 ctgtgggtgt tccggccgct gatcgaggac gcggttcccg ggatcagcct gaacgacacc
     1801 agcatcgcct tcttcggcgc gctggcgctg ttcgtgatcc cggtggatgc gcgcaagggc
     1861 atcttcgcgc tgtcgggcga gtgggcgagg aacctgccct ggggcgtggt gatcctgttc
     1921 ggtggcgggc tgtcgctcgc cgacggcatc aagagcagcg gccttgccga ctggatcggc
     1981 gccggcaccg gcgggctcgc caccctgccc accgtcgcgg tggtgctggc ggtggtggtg
     2041 ctgatcgtca tgttgaccga gctgaccagc aacaccgcga ccacggcgac cttcctgccg
     2101 atcgtcgcgg cgatcgcgat cggcatcggc gagaacccgt tcctgctggt cttcccgacc
     2161 gcgctggcgg cgagctgcgc gttcatgatg ccggtggcca cgccgcccaa cgcggtggtg
     2221 ttcgcgagcg gccacgtcac gatcagccag atgatccgca gcggcatcat ggtgaacatc
     2281 gtcggcgtcg tcgtcaccat gctgatcgcg tacgtcgcgg tgcgcgcggt gttcgggatc
     2341 gagctcggcg tgctgccgga ctgggccgcg aacgccggca agtgaccacg cgcctgttat
     2401 tggctgggcg gccgctccac gctccactcg ccgtcccagg tggtgacgaa ccgccgcggc
     2461 cccgtcgacg ggattgccgt ccaggtgccg gagccggcga tgccgtcgta gcggccggtg
     2521 ccgccgagga aatgccaggc gccgctgttg cgcgcgccgt cataggccat ccaccaggca
     2581 ttgccctgcg ggtcgatgcc gaggcactgg ccgtaaccgc ggccttccgg gtcgtcggcc
     2641 gggacgtaga agctgccggt gcactcgaag ctcgacttgt tgaagaccag gctcggatcg
     2701 gaggagaaat agaggccgct gcttcggcgc cggatgaccg tggcgccgtc ggcgaccttg
     2761 atctcggcct cgatcgtccg ttcgacggcg ctgaacccga gccattcccg cggctccgcg
     2821 gcagcgggga ctgcggcgag cgcaaggcac gcgatgagga gtctgttgat catgtgcgcg
     2881 gccccgacgt catgtcgact caccggccat ggtggcgcaa tacaggggct cggacaacgg
     2941 caatgctcgc catgcgcgcc gaaatcggtt tcccgcagcg cctgtcgggc ctaccttgac
     3001 ggcggtcgcg acgaacccga ggaatggccg aggccgatgg catcacggcg catattggcg
     3061 tggatttccg cggccgcggt cggcggctgg gcggccctgt gggtcgcggc attgtcaccc
     3121 gccgcgaccg ttcccgcgcc gtctggcccg atcgcctgcg ccggctgcga cctcgccggg
     3181 gtcgactggg cgcatcgggc gttggaaggg gctgtgctcg acggcgccga cctcagcggc
     3241 gcgcgcctga ccggggcgcg catggccggg gcgcggcttg ccggcgcgcg cctcgccggc
     3301 gccaacctgt tcggcgccga cctcaccggc gccgacctca ccggcgcctc gctcgccgaa
     3361 gccaatctcg ccagcggcgt gctcgccggc gccgacctcg ccggcgccga cctcagccgg
     3421 gcgaacctgg tcggcacggt gctgaccggc gccaggctcg acggcgcccg gctcgccggc
     3481 gcgttcatcc acgacgccta tctgtgcggc gtccgcttgc cggccgccgc cggtacgcat
     3541 caccgccgct gctgagcgcg cctcagccgg tggtctcgag ataggccagg atcttgcgcc
     3601 ggatgttcgc gtcgggctgg cggtagaaca tgcgaacgcc cgggatcatc gcccgcgcat
     3661 cggtcatcag ccggtcgagg ttgggcgcgg tccagaccag gtcccgggtc tcggcgacga
     3721 agctctccga gtaattgaag gcggggtccg cgccggcggc gcggccgacg atgccggcga
     3781 gccgcggccc gtaggtcgcc atcatcaccg ggaccaggcg cgcgccggga tcgcgccggc
     3841 ccgcgagcgg cagctcggcc gagaagtggc agtacgcgca ttcggtctcg tacagcgcgc
     3901 ggccgtcgcc ggcctcgtcg gcgaacgcgg ccggcgacgc catcgccgca agcagagccg
     3961 ccgccaggat gcgcgcgtgc gccatggctc cgatttacgc ctcggtggtg ccgagccgcc
     4021 gcgacgggcc gggcaccagg ttcgccaccg ccatgccggc ggccatggcg gcgacgaaca
     4081 gcgccgactc gacctggccg aaggcgaagc tcgcgatcgc cgggccgggg cagaacccga
     4141 ccaggcccca gccggccccg aacagcgcgg cgccggcgag caggcgcgcg tcgatgccgc
     4201 tcacctcggg cagggcgaag ctccgcgcca gcagcggcgc ccgccggcgc aggctgtagc
     4261 ggaacgcgac caggctgacc gcaaccgcgc cgcccatgac gaaggcgagg ctcgggtccc
     4321 agtcgccggc aaggtcgaga aaggcgagca ccttagccgg gtcgaccatc tgcgagacgc
     4381 cgaggccgag cccgaacacg atcccggaga ccgccgccac cagcatctgg gccatcacca
     4441 gcctcccgcg acgtggcgga cggcgaacac caccagcatc gccaccgcca tgaaggtgag
     4501 cgtcgcgacc agcgagcgcg gcgacaggcg ggcaaggccg cagacgccgt gaccgctggt
     4561 gcagccggcg ccgatgcgcg tgccgacgcc gaccagaagg ccgccgatca ggagcgcggg
     4621 cagcggtgcg gcgatggtga tcgaggcgcc ggggtcgatg accgtgcgat ggatcagggc
     4681 gccggcgacc aggccggcga gaaacaggcc gcgccaggcc gcctgctcga ccgagccggg
     4741 cgggacgacg ccggcgacga tgctgctgac gccggccagg cggccgttca tcagcatcag
     4801 cagcgccgcc gaggcgccga tcaagacgcc gccgatcaac gccgataccg gggtaaagtt
     4861 ctccacctgc cactccttgc ttcgccgacg cgtcgggcgc tcatgatgcc gcgatcccct
     4921 gcctcttgcc aagccggcgt caatcggccc acgcggggac gcggagtcgc ggagaaggcg
     4981 cggagcagaa tggtaccgcg acgacgccgg ccgagataag cgccaaggcc tccgcgtgaa
     5041 tcgaaagact ggcggccatc cacgcatctg agatggccgt gatgaggaga cgcgttccga
     5101 tccagccgac tttggtgtac gagacttgga acgacacccg gcatgcctca ccgaaatgct
     5161 gctcatagag aacctgcgct cgttgcgcgc ggacgtggcc ccggtctcgt tcaccctcga
     5221 cgacggcgcc tgcctcgccg tgcatgggcc ctcgggcgcg ggcaagacgc tgttgctgcg
     5281 cgcgattgcc gatctcgacc cgaacgaggg cgcggtcacg ctggacggca cgccgagaga
     5341 gtcgctgcgc gcgccgcact ggcgccggct cacggtctac gtgccgaccg agtccggttg
     5401 gtgggaggac acggtcgggc accatttcga ggatccggag gcggtgcggc cgctgctcga
     5461 cgacctcggc ctgccggccg acattcggca gtggccggtg gcgcggctgt cgaccggtga
     5521 gcgccagcgg ctcgcgctcg cccgcgcgct ggtgttgaag ccgcgcgtgc tgctgctcga
     5581 cgagccgacg gcggcgctgg accctgaggc ggcggcagca gccgaggcgg cgatcgcgcg
     5641 gctgctggca gtgggcgcga gcgcgctgtg ggtctcccac gatcttgccc agcgccggcg
     5701 ggtcgcggcg cgatccctgg cgatccctga cgggcgcatc gaggaccgcg cgcggtgaca
     5761 tacatcgagc tcgcctatac cgacctcgcc atcgcctcgc tgctggtggt gctcaacgcg
     5821 ctgctgtcgc tggtgctggc gctcaggatg cagcagcagc tcctggtcgc gggcgcgcgc
     5881 atgatcgtgc aactcgccct ggtcgcggtc gtgctcaagg tcctgttcgc gcacgtctcg
     5941 ccgctgtgga ccgggcttgc cgccgcgacc atgatcctgt tcgccggccg cgagatcgtc
     6001 gcccgccagg agcgccgcct cgccggcctg tgggcttacg gcctcggcac cggctgcatg
     6061 ctgctcgccg cgtgcctggt cacggtgttc gcgcttacca cccagatccg ccccgatccc
     6121 tggtacgacc cgcgctacgc cctgccgttg ctcggcatgg tgctcggcaa caccatgacc
     6181 ggcatcagcc tcggcctgca cacgctcacc accgggctgg cgcgcgaccg ggtcgcggtc
     6241 gaggcccggc tcgcgctcgg cggcagcttc tggcaggcgg cgctgccgac cacgcgctcc
     6301 gccatgcgca gcggcctgat gccgatcgtc aacgccatgg cggcgaccgg gctggtgtcg
     6361 ctgcccggca tgatgaccgg ccagatcctc gccggcgcgg acccggtgca ggcggtgaag
     6421 taccaggtgc tgatcatgtt cctgatcgcc ggcggcaccg gcatcggcgt gctcttggcg
     6481 gtggccggcg ggctcgcgcg cctgaccgat gcgcgccagc gcctcaggct cgaccggctc
     6541 gggccggcgc gcggctgagc gcgcggtccc ggagccgacc gtcgcggcca tgtcgcccga
     6601 tagtccgttt ggatcatgac aggggcggcg tccgagcgcc taatcggcaa cgtgcttcgt
     6661 gcgctggccg cacaaggagg tcgcgatgag agccgtgtcg accgcccgct cgctggccga
     6721 gtccgacaac gaaatgatcc gcgtgatcga ggacctggtc acgctgctga tcgacaagaa
     6781 ggtgatcagg tcgggcgagc ttccggtggc ggcgctcgag aaggtgatca agcgccgcct
     6841 gtggcgccac cgcatgaacc ggcgcccggc ggagccgctc gagtaggccg acgaaaaaag
     6901 aaccccgccg gttggcgggg ttgagggcaa tgcgacgccg cggcgccgct cagccgagct
     6961 gctggtaggc gtgggccgcg cgcagcacgg tcgcgtcctc gcccagccgg ccgatcagca
     7021 tcatgcccac cggcagcccc tccgacatcg cgcatggcac gttgatcgcc gggttgccag
     7081 tgacgtcgaa cgggcaggtg ttgtggatca tgttgagcgc gtgggcgacg atctcggtct
     7141 tgctcgcgcc tgcgggcggc agcaccgtcg ccttctgcgg cgtggtcggc atgaccaaga
     7201 ggtcgacgcc ggcgaaggcg tcgtcatacg cctcgcgcag cactcgggcg aggttttgcg
     7261 ccttggcgta gtagcgtccg tggtacgcct gctgcatgta ctcgccgagc agcagcacca
     7321 gcttgcaggt ctccgagtag tcgtccgcgc gcacccgccg gctgcggccg tagaagtcga
     7381 gcagctgggt gaggtagtgg ccttgccagt tggtgcccat gctgttgccg tcgatcatca
     7441 gcatgtgtgc gccctcgacc gcgatcgcgt tccagacatg gacgccgtcg cggtgcatcg
     7501 gcaccgagat ctcccgcacc tcggcgccgg cgtcgccgag ccgcatggcg gactcgcgca
     7561 cgatccggtc gacgtcgggc tcggacaagc cttcccagcc gaagccctcg gcgacgatgc
     7621 cgatcttgag gccgttggca tcgccggtca gcgccttggt gtagtcctgg ccctgccagc
     7681 cgcgcgcctg gcgcggatcg agcccgtcgg ggccggcgag cacctcgagc agtagcgcgc
     7741 agtccgccgc cgagcccgcg atcgggccgg tgtggtcgag ggtgagctcg atcgggaaca
     7801 ccccggtata gggcaccagg ccgtaggtcg ccttgaggcc gaacgcgccc gagtacgagc
     7861 tcgggatgcg gatcgagccg ccctggtcgc cgccgatcgc catgtcgacc gcgcccgaga
     7921 cgaccagcgc cgcgctgccc gacgacgacc cgccggccga gcgcgtcgcg tcgtgcgggt
     7981 tgagcacgta gccggtgtcc gaggtgtggc tggaccccga gaagcagaag tgctcgcata
     8041 ccgccttgcc gacgatctcg ccgccggcgt cgaggatgcg ggtgacgatc gtcgcgtcct
     8101 ggtcgggaac gaagccttcg agcacgctgg caccgttcat catcggcacg cccgcgacgc
     8161 agacattgtc cttgatcgcg atcttcttgc cggcgagctt gcccgaggcc gcgcccttga
     8221 tcgtgcagcg ccagtaccag gcgttgagcg ggttctcgct ggcgggcggc cggtagcccg
     8281 ggtcgcgcgg gtatttcacc ggcagccggg gctcggcgta gaggtcaagt cgcctatacg
     8341 atcccaggat cccggccatc aacgaccggt attcctcaag gtcctcggtc gtaaggtgaa
     8401 ggtggaactg gcgtgcgacg tgctcgagct gacggatcgt cggcggctgg accgagctcg
     8461 gcggcaccca tgcgggcggc gggctctgcg cggcggccca ggactcgccg gccaccgcgg
     8521 acagcgccat cggc
//
LOCUS       DASWHU010000221         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_487193, whole genome shotgun sequence.
ACCESSION   DASWHU010000221 DASWHU010000000
VERSION     DASWHU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487193"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VGB88_08250"
     CDS             complement(<1..882)
                     /locus_tag="VGB88_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647474.1"
                     /translation="MSRIARAIGAVELAAADLLAGASALIGRRVIANAEKIVAARDRG
                     LTVAAVAVALIAAPLVLGHRVPLTTWARDYALPLDTSLRWAAGQVPHLDYGTPVGAGY
                     WLVQGAAVELIGLVGTSPVIANLIAAVVLAPLAWLLLRARLRGGLVGLVLLAACAMIV
                     SPRALGDEISLVSFLGAYNKYGLAILAVLVAALFVARPGRPAPTEWAGDAAIIAVLMV
                     CLVYLKVTFAAIAAAGAVAALHYAPGNRRAILAGLALAAALVVAVGAATGLNGPYLAD
                     LRSAAGAAEALRAKKLFT"
     gene            complement(879..1610)
                     /locus_tag="VGB88_08255"
     CDS             complement(879..1610)
                     /locus_tag="VGB88_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9647475.1"
                     /translation="MNLVITMAGAGRRFRDAGYDQPKFAIRVKGRSLFTWAMQSLDAF
                     LTPAVRAIFVARAGDAAADFVAGEMAAWPGVEARLVELDHITDGQATTALIAAAHWDE
                     AAPLLIYNIDTHVNPRHLRPQMIAGEGWIPCFRAAGEHWSFVRTDAHGRALEVAEKRR
                     ISDLASIGLYWFASAGLFARAYAETYESAGAAPELAERYVAPVYNALIAEGRAVTVTE
                     VPAEAVVPLGTPAEVRRFEAECAAR"
     gene            complement(1607..2014)
                     /locus_tag="VGB88_08260"
     CDS             complement(1607..2014)
                     /locus_tag="VGB88_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEX9647476.1"
                     /translation="MSERGALVIDVDGTLCPVKGADQRYEDLEPNRDVVARLGEWRRM
                     GYRIVLHTSRNMRTHQGNLGLINKHTAPVLLAWLARWEVPFDELHFGKPWAGDDGFYV
                     DDRAVRPDEFLAHSPDELRALVEAARARIGGGR"
     gene            complement(2007..>3341)
                     /locus_tag="VGB88_08265"
     CDS             complement(2007..>3341)
                     /locus_tag="VGB88_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647477.1"
                     /translation="GVDEITIIEDTRDLERLRARLRAGPQDGCFVHFRARGAIADRDG
                     LALLLRKVALADGILFDGPHDPLLAACHGAGAYDSYLELAESGLAVGAPVGAQDFDPP
                     AQLVLPDDALVDLTDLPTFVAYFSAGFETRHFNRIQGDDVTVIKRSADRAKIEAEYRY
                     LTMLPEEVKRWFVQAHGFRVDGNVASYRMERLGIADVALIWIHGAMDGDAFAPFLDKL
                     FRYIDERPSRATDRDRYVAAARSLYVDKVRARIARLAQEPAFAALDSHIRAGTAYDGV
                     AAVVEDYVGRFEAYLAGRGRDDAPRAVIGHGDLCFSNILYDKASRLMKFVDPRGAADE
                     AGMWSDPFYDLAKLSHSIFGSYDFLANDLFTIEVSDTLEVELAVSGPAPVAQQAQFQQ
                     ALEARGYDLYGIRLREASLFLSMLPLHLDSPRKVLAFALDGISILNELARNE"
BASE COUNT          513 a         1184 c         1217 g          427 t
ORIGIN      
        1 ggtgaacagc ttcttggcgc gcagcgcctc ggccgcgccg gcggcgctgc ggagatcggc
       61 gagatagggg ccgttgagcc cggtcgcggc gccgaccgcg accaccagag ccgcggcgag
      121 cgcgagcccg gccaggatcg cgcgccgatt gcccggcgca tagtgcaggg ccgccaccgc
      181 gcccgcggcc gcgatcgcgg cgaaggtgac cttcaggtag actaggcaaa ccatcagcac
      241 ggcaatgatc gccgcgtcac cggcccactc ggtcggcgcg ggtctgcccg gccgggcgac
      301 gaacagcgcc gcgaccagga ccgcgaggat cgccagcccg tacttgttgt aggcgccgag
      361 gaacgacacc aggctgatct cgtcgcccag cgcgcgcggc gagacgatca tcgcgcacgc
      421 cgccagcagc accaggccga ccagcccgcc cctgaggcgc gcacgcagca gcagccaggc
      481 gagcggcgcc agcaccacgg ccgcgatcag attggcgatt accggcgagg tgccgacgag
      541 cccgatcagc tcaaccgccg cgccctggac cagccagtag ccggcgccga ccggcgtgcc
      601 gtagtcgagg tgcggcacct ggcccgccgc ccagcgcagc gaggtgtcga gcggcagcgc
      661 gtagtcgcgc gcccatgtgg tcagcggcac ccggtggccg agcaccagcg gcgcggcgat
      721 cagcgccacc gccactgccg ccacggtgag gccgcggtcg cgcgcggcga cgatcttctc
      781 ggcattggcg atcacccgcc ggccgatcag cgcgctggcg ccggcgagaa ggtcggcggc
      841 ggcgagctcg accgcgccga tcgcgcgcgc gatgcggctc atcgcgccgc gcactccgcc
      901 tcgaaccggc ggacctccgc gggcgtcccc agcggcacca cggcctcagc cggcacctcg
      961 gtcacggtga ccgcccgccc ctcggcgatc agcgcgttgt agaccggcgc gacgtagcgc
     1021 tcggcaagct ccggggcggc acccgcggac tcgtaggtct cggcataggc gcgcgcgaac
     1081 aggcccgcgc tggcgaacca gtagagcccg atcgaggcga ggtcggagat gcgccgcttc
     1141 tcggccacct cgagcgcgcg cccgtgcgca tcggtgcgca cgaagctcca gtgctcgccg
     1201 gctgcgcgga agcacgggat ccagccctcg cccgcgatca tctgcgggcg caggtggcgc
     1261 ggatttacat gggtgtcgat gttgtagatc agcagcggcg ccgcctcgtc ccagtgcgcc
     1321 gcggcgatca gcgcagtggt cgcctggccg tcggtaatat ggtcgagctc gaccaggcgc
     1381 gcctcgaccc cgggccaggc ggccatctcg ccggcgacga agtccgctgc cgcgtcgccc
     1441 gcgcgggcaa cgaaaatcgc gcgtaccgca ggcgtcagga acgcatccag gctctgcatc
     1501 gcccaggtga acaggctccg ccccttgacc cggatcgcga acttgggctg gtcgtagccg
     1561 gcgtcgcgga agcggcggcc ggcgcccgcc atggtgatga ccaggttcac cggccgccgc
     1621 cgatgcgcgc ccgcgccgcc tcgaccagcg ccctcagctc gtccggcgaa tgggccagga
     1681 actcgtccgg ccgcaccgcc cggtcgtcga cgtagaagcc gtcgtcgccc gcccacggct
     1741 tgccgaagtg gagctcgtcg aacggcacct cccagcgcgc cagccaggcg agaagcacgg
     1801 gcgcggtgtg cttgttgatc aggccgaggt tgccctgatg tgtgcgcatg ttgcgcgatg
     1861 tgtggagcac gatgcgatag cccatccgcc gccattcgcc gagccgcgcg accacgtcgc
     1921 ggttgggctc gaggtcctcg tagcgctggt ccgcgccctt gaccgggcac agcgtgccgt
     1981 cgacgtcgat caccagcgcg ccgcgctcac tcattgcgcg cgagctcgtt caggatcgag
     2041 atgccgtcga gggcgaaggc gagcactttg cgcgggctgt cgaggtgcag cggcagcatc
     2101 gacaggaaca gcgacgcctc gcgcagccgg atgccgtaca ggtcgtagcc gcgcgcctcg
     2161 agcgcctgct ggaactgggc ctgctgggcg accggcgccg gcccggagac ggccagctcc
     2221 acctcgagcg tgtcggagac ctcgatcgtg aacaaatcgt tggcaaggaa atcatacgac
     2281 ccgaagatcg agtgcgacag cttggcgagg tcgtagaacg gatcgctcca catgcctgcc
     2341 tcgtcggccg cgccgcgcgg atcgacgaac ttcatcagcc ggctcgcctt gtcgtagagg
     2401 atgttcgaga agcacaggtc gccgtggccg atcaccgccc gcggcgcgtc gtcgcgaccg
     2461 cgccccgcca ggtaggcctc gaaccggccg acatagtcct cgaccacggc ggcgacgccg
     2521 tcataggcgg tgccggcgcg gatgtgcgaa tcgagcgcgg caaaggcggg ctcttgcgcg
     2581 aggcgcgcaa tgcgcgcgcg caccttgtcg acgtagagag agcgcgcggc ggccacgtac
     2641 cggtcgcgat ccgtggcgcg cgacgggcgc tcgtcgatgt agcggaacag cttgtcgagg
     2701 aacggcgcga acgcgtcccc gtccatcgcg ccgtggatcc agatcagcgc cacgtcggcg
     2761 atgccgagcc gctccatccg gtagctcgcc acattgccgt cgacgcggaa gccgtgcgcc
     2821 tgaacgaacc agcgctttac ctcctcgggc agcatggtca ggtagcggta ctcggcctcg
     2881 atcttggcgc ggtcggccga gcgcttgatg acggtgacgt cgtcgccctg gatgcggttg
     2941 aagtggcggg tctcgaagcc ggccgagaaa taggcgacga aagtggggag gtccgtgagg
     3001 tcgaccagcg cgtcgtcggg cagcaccagc tgcgcggggg ggtcgaagtc ctgggcgccg
     3061 accggggcgc cgaccgcgag gccgctctcg gcgagctcga gataggagtc gtaggcgccg
     3121 gcgccgtggc aggcggcgag cagcgggtcg tgcgggccgt cgaacaggat gccgtcggcg
     3181 agcgcgacct tgcgcagcag cagggcgagg ccgtcgcggt cggcgatcgc gccgcgggcg
     3241 cggaaatgga cgaagcagcc atcctgcggc ccggcgcgga ggcgggcgcg caggcgctcg
     3301 aggtcgcgcg tgtcctcgat gatggtgatc tcatcgacgc c
//
LOCUS       DASWHU010000222         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1978742, whole genome shotgun sequence.
ACCESSION   DASWHU010000222 DASWHU010000000
VERSION     DASWHU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978742"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VGB88_08270"
     CDS             complement(<1..1140)
                     /locus_tag="VGB88_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9647478.1"
                     /translation="MAEGGAGRARIADYRYGRLLIRYRWAAIALTLIAVAASAAGLPR
                     LAMNPDSRVFFGKNNPQLKALDQFENTYTKTDNVMYVLAPRQGTVFTREVLDAVAWLT
                     EESWQTPYSRRVDSITNHQHSYARGDELVVEDAWPGGEAATDADLAAARAAVMDSPQL
                     VDRLVSSDETVTAVNVIVVTPGSKLQEVPEIAAFARARAAEFAARYPDIDLHLVGGVM
                     IDMAFNEVPQGDIATLFPIMIVAIFVIIGVTVRSVTWSLLMLATVIATVLITLGVAGW
                     AGVVLNAGTMGAPIIMLTLAVAHGVHISVSAQQQLRKGVAKHDALVESLRINMSPVFI
                     TSATTAIGFLSMNFSDAPPFRLLGNLVATGVMVAFVLSVFSLPAAM"
     gene            complement(1133..>1637)
                     /locus_tag="VGB88_08275"
     CDS             complement(1133..>1637)
                     /locus_tag="VGB88_08275"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647479.1"
                     /translation="MSAANIYRYFDGKGDIAAVGTGRWLAALETELEGLADDAARAPA
                     ERLRRLVLAKLEAMAALIRSAPHLDELIDHVCSAREEVVAGHRLAVRALYARVIRAGQ
                     RAGAFAAGDADWAAAAFDAATCGFFHHSLLRAQAADKVRAEAEAALALLIAGLSAGPY
                     GEAAGDG"
BASE COUNT          252 a          567 c          588 g          230 t
ORIGIN      
        1 catcgccgcc ggcagcgaga ataccgacag cacgaacgcg accatcaccc cggtcgcgac
       61 caggttgccg agcagtcgga aggggggcgc gtcggaaaag ttcatgctca ggaagccgat
      121 cgcggtggtg gcgctggtga tgaatacggg cgacatgttg atcctgagcg attcgaccag
      181 ggcgtcgtgc ttggcgacgc ccttgcgcag ctgctgctgg gcgctcacag aaatgtgcac
      241 gccgtgggcg acggccaagg tcagcatgat gatcggcgcg cccatggtgc cggcgttcag
      301 caccacgccc gcccagccgg cgacgccgag ggtgatgagc acggtcgcga tcaccgtcgc
      361 cagcatcaac agcgaccagg tcaccgagcg cacggtgacg ccgatgatca cgaagatcgc
      421 cacgatcatg atcgggaaca aggtggcgat gtccccttgc ggcacctcgt tgaatgccat
      481 gtcgatcatc accccgccga ccaggtgcag gtcgatgtcg gggtagcggg cagcgaactc
      541 cgccgcgcgg gcgcgggcga aggcggcgat ctcgggcacc tcctggagct tgctgccggg
      601 ggtgacgacg atgacgttga ccgcggtcac ggtctcgtcc gagctgacca ggcgatcgac
      661 cagctggggg ctgtccatca ccgccgcgcg ggcggcggcg aggtcggcat ccgtggccgc
      721 ctcgccgccc ggccaggcgt cctcgaccac cagctcgtcg ccgcgggcgt agctgtgctg
      781 gtggttggtg atcgaatcga cgcggcgcga atacggggtt tgccagcttt cctcggtcag
      841 ccaggcgacg gcgtcgagca cctcgcgggt gaacaccgtg ccctggcgcg gcgccagcac
      901 gtacatgacg ttgtcggtct tggtgtaggt gttctcgaac tgatccagcg ccttgagctg
      961 gggattgttc ttgccgaaga acacccggct gtcgggattc atggcgagcc gcggcaggcc
     1021 ggccgccgac gccgccaccg cgatcagcgt cagcgcgatc gccgcccagc gatagcggat
     1081 cagcagccgg ccgtagcggt agtccgcgat gcgcgcccga ccggcgccgc cctcagccat
     1141 cgcccgcggc ctctccgtag ggcccggcag agaggccggc gatcagcagc gccagcgccg
     1201 cctcggcttc ggcgcgcacc ttgtcggcgg cctgggcgcg caacagcgag tggtggaaga
     1261 agccgcaggt cgcggcgtcg aacgccgccg ccgcccaatc ggcgtcgccg gcggcgaagg
     1321 cgccggcgcg ctggccggca cggatcaccc gcgcgtagag cgcgcgcacc gccaggcgat
     1381 ggccggccac cacctcctcg cgcgcgctgc acacgtggtc gatcagctcg tccagatgcg
     1441 gcgcgctgcg gatcagcgcc gccatcgcct cgagcttggc cagcaccagg cggcggaggc
     1501 gttcggcggg cgcccgcgcg gcgtcgtccg ccagcccctc gagctcggtc tcgagcgcgg
     1561 ccagccagcg gccggtgccg accgcggcga tatcgccctt gccgtcgaag tagcggtaga
     1621 tgttggccgc cgacatg
//
LOCUS       DASWHU010000223        21902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2349111, whole genome shotgun sequence.
ACCESSION   DASWHU010000223 DASWHU010000000
VERSION     DASWHU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349111"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1430)
                     /locus_tag="VGB88_08280"
     CDS             complement(306..1430)
                     /locus_tag="VGB88_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9647480.1"
                     /translation="MKIKAAVLYEMGQPEPYAQSRPVQIEELDLDGPGAGEVLVKVAA
                     AGLCHSDLSVINGARPRPLPMVLGHEASGVVAEVGAGVADLKPGDHVVFVFVPSCGHC
                     VPCAEGRPALCEPGAAANAAGTLLAGGRRLHKGAAALNHQVGVSCFAEYATVSRRSLV
                     KVDRELGLEEAALFGCAVITGVGAVVNTARLRPGASVAVVGLGGVGLNALLGARMAGA
                     RRIVAIDLLDDKLALARQLGANDTFNAAADDVVEAVRAATGGGADYAFEMVGNVKAME
                     LAYRITRRGGETVSSGLSHPDHSFSVQHVNLVGEERTIKGSYLGSCVPQRDIPNYIAL
                     YQQGRLPVDRLMSERVGLDEVNEAFDRLASGHTVRQLIVF"
     gene            complement(1467..2162)
                     /locus_tag="VGB88_08285"
     CDS             complement(1467..2162)
                     /locus_tag="VGB88_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647481.1"
                     /translation="MPTILITGANRGIGLALAKAYAADNWLVLATCRHPGKADELRAL
                     AKAHEGVEVRALDVAEPRAIREFAAWLEGLPIDVLFNNAGMLGPRDGQSLGDIDYAGW
                     AEVLRVNLLAPVAMAETLADNVAASDRRVIAMMSSSMGSMAENKGGGYYLYRSSKAAL
                     NMAVRSLAADLRRRRVIVVALHPGWVRTDMGGAGASLSPGRSAAGLKRVVDSLKLDDS
                     GKFRRYDGSALPW"
     gene            complement(2170..3102)
                     /locus_tag="VGB88_08290"
     CDS             complement(2170..3102)
                     /locus_tag="VGB88_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9647482.1"
                     /translation="MANGRKVIITCAVTGSIHTPSMSPHLPVTPDEIAEAAVAAAEAG
                     AAILHLHARDPDDGHPTQDPKVFDRFLPRIKQATKAVINITTGGAPTMTVQERLTPAA
                     TFKPEVASLNMGSMNFGLFPMLKRFNEFRHDWERRHLENSRDMVFKNTYADIEFILSS
                     CRGNGTRFEFECYDIAHLYNLAHFLDRGLVEPPLFVQSVFGILGGIGTHPEDLMHMRR
                     TADRLFGDSYLWSILGAGRNQIPLATIGAAMGANVRVGLEDSLWLGPGRLAESNAAQV
                     TRMRQVLDGLSLDIATPDEARAMLALKGGDAVAF"
     gene            complement(3122..4072)
                     /locus_tag="VGB88_08295"
     CDS             complement(3122..4072)
                     /locus_tag="VGB88_08295"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9647483.1"
                     /translation="MADRIAVVGAGLIGRAWAIVFARAGHEVALYDPADGVVAGALEA
                     IAAALPELAEAGLLGGDAPAAVQARIHAAATLAAALDGAGHVQENGPEKVEVKRALFA
                     ELDRLAAPHAVLASSTSGIRASRFTAELPGRARCLVAHPINPPHLVPLVEIVPAPWTD
                     PGAVARTAALMGAVGQAPITLKREVDGFVANRMQGVLLGEAFKLVEDGVCDAADVDTA
                     IADGIGLRWAFMGPFETIDLNAPGGVRDYCERFARMYHGLASEDPEPRLMVGAGLDRV
                     EADRRKALPADKLAERARWRDRRLADLVAAKLRAARTIGR"
     gene            complement(4097..4855)
                     /locus_tag="VGB88_08300"
     CDS             complement(4097..4855)
                     /locus_tag="VGB88_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647484.1"
                     /translation="MISAGAAGIGRATAAAFAAAGAKVHICDVDEQALAACLAEVAGV
                     AGVPADVADDGQVGRWFDAARDALGGLDVMVNNAGIAGPTGPIESLDAAEWRRTIEVN
                     LTSMYLCCRRAVPLLKAAAAEHGEAVMVNLSSVAGKFGYAFRTPYSASKWAVVGLSKS
                     LSRELGPFGIRVNAVQPGPVAGPRIERVIEAKAAAQGVSPDEIRAALTAHASLGRFVT
                     ADDIAGTILFLSSPQARNISGQAIAVCADSTDIT"
     gene            complement(4979..5896)
                     /locus_tag="VGB88_08305"
     CDS             complement(4979..5896)
                     /locus_tag="VGB88_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647485.1"
                     /translation="MVGNEGAVLCACRGRDVIERAYAPSPDAAGELRSMLAAAPRWPV
                     TVMVDVLEISLQRAALPPVAAWQTGSLLRRRMARAFAGFRATGALAEGRAREGRRDRE
                     FLIAGVAPSAGYDGWMAFLDGVPNRVGAVVLLPVEAAVMLERLAAPLGEARSAWQVMV
                     SRERTGGFRQVIAADGRIAFSRLTRSLDPDTPADAVAEQIEQEFRHAIDYAKRLGFRQ
                     GDGIDLIAVTAEDEKAALARRPLPASHLLVLTPFEAAAKLGLGRSVAPGEGYADVLHA
                     AALALRGRPRLKLVPERRGRVRAPADAVR"
     gene            complement(5943..7148)
                     /locus_tag="VGB88_08310"
     CDS             complement(5943..7148)
                     /locus_tag="VGB88_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9647486.1"
                     /translation="MPDYAYRAMDGRGRVVRGTMSAANALDLESRLGGLSLDLLSERP
                     LRAGRLGALLGRRVRITDMMQLCIHMGQLLRAGVPVLDCLSGIRDVTGSPELRDVLSA
                     AHNDVRDGQMLSGAFARHPRAFSGEFVGLVRAGEETGNLADSFADLARHLRWLAELRQ
                     RIRRALRYPLVIGLLLAAVFALMLGYVVPSVVGYLREVGVGLPFLTVALLAVSEFFIA
                     AWPALVAVPVALWAAAALARRASPAAAVAIDAAKLRLPLLGPVLRRLALSRFSHYFAV
                     LLRGGVPLLASLETARGVVGNLRIAAAVAAATERVASGHSLARALESTGEFPSLVVRM
                     VGVGEEAGALLEALDNVTYFYDREVDDAVSALIAALEPAVTAVLGALLAWVAVAVLGP
                     IYNSFGALG"
     gene            complement(7158..8972)
                     /locus_tag="VGB88_08315"
     CDS             complement(7158..8972)
                     /locus_tag="VGB88_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9647487.1"
                     /translation="MPADLHFKGPSPPAPPASAGAGATWRRLGAAAGERGAEARAEGA
                     DPAASLRLGERLRELRLLSDDQLEVALLEQKRSGKLLGEILVDFGFITEGALSQVIAE
                     STGRARFDAGTTVVDPDVVQLVPKEIALRHKVLPVAVVGDTLLLAMADVYNVMAIDQV
                     RHHLPARLRIEPLVSTDVDLQAAIDQSYGYQMSIAGILRELETGEREASGGEQEGYVN
                     PTVRLVNAILVDAVKRGASDIHFEPEGAFLRLRYRIDGVMIPVRAFHKDYWPAIAVRI
                     KVISGLDIADSRNPQNGRLSYLMSGREVDFRVASQPTVHGENIVMRILDKARSLLPLE
                     ELGFGEANVALLKRLLRRPEGIVFVSGPTGAGKTTTLYSILRYLNATDVNIMTLEDPV
                     EYQLPLIRQANVREDTAMNFAEGIRSMLRQDPDILFIGEVRDQDTGLMALRAAMTGHQ
                     VFTTLHSNDAVGVIARLIDIGVPRHLLAGQIICCVAQRLVRILCPECRRPRPARADEC
                     ELLGGDPAAPPTVHDPVGCAACDQLGYRGRTAIVEILPVTDDLDELIASGGTRTAMLA
                     SASRSGFRPIAEDGIDKVLAGLTSVEAVARAVNLTGRR"
     gene            complement(9077..9781)
                     /locus_tag="VGB88_08320"
     CDS             complement(9077..9781)
                     /locus_tag="VGB88_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9647488.1"
                     /translation="MRAQRGFTLVELSIVLIIIGLLIGGILKGQELIRNAQSKALITQ
                     IDSFRAANNGFIDRYGQIPGDFSRASSLLPARSDGSSAQSDGDGNGRIAGLGNDGESL
                     EYWQHLAAADMIGGIAGIAPVPGVGVPVGRVPGSAWNAVNAATPGAELCADCTLRGNY
                     WRLGAPDENALTTVALLPADEAYLLDLKIDDGRPNTGLVQAGDGAGPGATPCERDGQY
                     LTAAGGAPACVMGFAF"
     gene            complement(9973..10716)
                     /locus_tag="VGB88_08325"
     CDS             complement(9973..10716)
                     /locus_tag="VGB88_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX9647489.1"
                     /translation="MPLAAVAVLAGVAGLAFGSFAALAAYRLPRGEGVVLGRSRCPRC
                     GHALALADLLPVVSWLALGGRCRYCAAPVPVRYPLIELATAALFVLAVLAWGVTPEAL
                     LACALALGLVIAAAADLDRLVIPDPVLVCLLPLGLAWAAVLGRPWSDTIGGLAAALAL
                     AAALRWLGARARGREALGLGDVKLLAVAGAWVGLAALPWYLAGAGALGVASGLAWRRR
                     GGEAFPFGPALAAALFATVLWRAPWPGWW"
     gene            complement(10725..12020)
                     /locus_tag="VGB88_08330"
     CDS             complement(10725..12020)
                     /locus_tag="VGB88_08330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9647490.1"
                     /translation="MKSPRHATVPHAQRRRAPGTGGARAAVAVVAVLAAAYVLSQFYR
                     TSVGVIGPELMATLGLSASALGTLGGSYFLAFAVAQVPVGVALDRFGPRRVSAVLLVV
                     AALGAAVFAAAPDAGVLTLGRGLIGIGCAAGLMGALVVFSRWFEARRFSTLAGLMLSV
                     GGLGALVSTAPLAYAAAALGWRGAFLAAAALTLAVAALVWLVVRDAPPGRAFHDRGRE
                     SVAEAARGVATVLGNRELHRLLPLNAVAYASVVAVLGLWGAPYLTDVHGLEAVDAGMV
                     MMGMAVMLMAGSISYGWLAPRLGGYKRPALAGAGGVAAIYAVLGLGLPLGLGAVAALL
                     ALAGLLGAYSVLLISHVRGLFPDHMVGRGLTAANLFNFGGVGLVQMASGWIVDAWPAE
                     AGAHPPAAYGALFWFLAAIIVLAALVYARARDPLGEAPR"
     gene            12079..13194
                     /locus_tag="VGB88_08335"
     CDS             12079..13194
                     /locus_tag="VGB88_08335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9647491.1"
                     /translation="MTAARATSYQPPDPAQAEVRAMPVAGEPLAACPVTFSAFSVCDH
                     HPALARTLDQFYRELLGHAVLAEELGYDAYWVAEHHFHEYGVVPNPAPFLAAIAQATR
                     RIRIGPAVSVLPFHDPVQVAEDYAVVDQLSGGRLNMGVGSGYLPHEFAGFGVGGDTKR
                     ARFDEALVLIRRLWAGETVTFEGAFHRVHGVALNVLPVQRPAPPLLVAALRPEACYHI
                     GRHGLNMMTIPYAAVDRMDGIGDMVADFRRGWQETQGTPPPVPLCALHAHVAASDEAA
                     RANAEAAFNQYTRTRLYAKSKKDYDDILAAELGLFGSVETVVGQLRRLHAMGARQVML
                     LQDFGALAAGKVRESMRRFATEVAPRLAERVRADAAA"
     gene            13216..14238
                     /locus_tag="VGB88_08340"
     CDS             13216..14238
                     /locus_tag="VGB88_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647492.1"
                     /translation="MLELRHARWIAVAAALAAPAHAAEIELVATHFVSAQHGVQVDML
                     GPWAAELERRTGGRVHVTIHPGGSALGNINKQYDQVRAGVTDIAFGLNGIPRGRFPRT
                     SIMALPFMTASADAATRALWDVFPEYLAEEYPGVHVLALTAHNGGMIHTVDKKVTTAE
                     DLAGLRIRAPSPEVTSMLEYLGASPVGMPPGEVYESLQRGVLDGAIFPWDPVKSMHIA
                     EVVRYHLEIGCYTVPFYLVINERRWQGLPAEVRQAIDDISGPALIEQFGGWWDKWDAA
                     GRAAAVERGNVISTLSDAERAAWRLRLEPMIEAWLAGLEDQGVDNAREIFDAMRARIV
                     VHEAAH"
     gene            14295..14732
                     /locus_tag="VGB88_08345"
     CDS             14295..14732
                     /locus_tag="VGB88_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9647493.1"
                     /translation="MLALVAAMLLTVVDVALRRPFDIAIKGTVDLTQLLVMAAAWLAI
                     PYAFLTDGHVSVPLLVDRLPARLRAGLAVLAALLGAALLAGIAWYGTAQARLEHGYGE
                     ISLTLGIAKHWYWLPLIAGAALSVAACAVRALAQAWRPRPGRG"
     gene            14734..16032
                     /locus_tag="VGB88_08350"
     CDS             14734..16032
                     /locus_tag="VGB88_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9647494.1"
                     /translation="MSDPAIGVAGFAVLLVLIALGVPVAVAMGAVGVVGFAVLGGWDG
                     AAFVLGSVPFQAVYPYSLAVVPLFILMGAFASRAGLSRALYDGVDSLVGHWPGGLAMA
                     TVGACAGFGAICGSSLATVATMARVALPEMRRHGYDDRLAAGAVAAGGTLGVLIPPSI
                     LLVVYALLTEQSIARLLVAALVPGLLATALYMAAVAVQVRVRPHLAPARERRPPARRL
                     AAALGMWQVALLFAAIIGGIYLGVFSVTEAAAVGAFGAVGFALAAGVGRASVVDGLAE
                     AASTTAMIFLILVGAGLFNFFVDASSVPIWLAERIAALAWPPTAVVVALMLFYLVLGC
                     FMDSLSMMLLTVPFVFPVVLAMGIDPIWFGVLLVTAVEVGLITPPIGLNLFVIKSVEP
                     TLGLGVVMRGSIPFVAADLVRIALLVALPQLSLWLPGRMG"
     gene            16226..16927
                     /locus_tag="VGB88_08355"
     CDS             16226..16927
                     /locus_tag="VGB88_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9647495.1"
                     /translation="MRQLIAVSEVLDRIIAKIGKLAAWLLIPMVGIIVIDVVTRKFGL
                     LSKLREALEASGAIEAAQFVQSYMTSTKFQELEWHLHAALFLLCLGFGYVRNSHVRIE
                     LVRERFGVRTRAWLELVGCMIFLVPYAGLVLYFGIDFSQRSFEMSEVSSALTGLSHRW
                     VIKAFVPLGMVLLLMATAAVVLRNVIFLFGSGALQGRALGEAMELHSAMQDLVASTVR
                     EAEEILAVEKKEPAS"
     gene            16924..18291
                     /locus_tag="VGB88_08360"
     CDS             16924..18291
                     /locus_tag="VGB88_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9647496.1"
                     /translation="MTFVDFLPVFMFFSLAILLFSGYPVAWVLGGIGVSFGAIGMLFG
                     VFSFVEYFNIISRIWGTAAENLILVAVPMFIFMGTMLERSGVANDLLYALQVLLRRTP
                     GGLALSVTIMGTIMAATTGIIGASVVMMTLLALPVMLDRGYDIPLATGTIAASGTLGI
                     LIPPSIMLVIMADLLSISVGTLFLAAIVPGLILSGLYLVYIYIATRIKPSLAPPLPDE
                     MGPDTRAQLVTMVLRSFFPPIMLIVFVLGSIFAGWATPTEAAGVGALGAVLLAVFNRK
                     FSFTVLKDVSERSALTIAMIFGIFIGATSFSYVYRSLGGDDLVHAFVDHVGFGSWGLL
                     ILMMVLVFLLGFFFDWIEITLIVLPLFAPIMRTMDFGTHIPFQVEVIPWIAILLAVNL
                     QTSFLTPPFGFALFYMKGVAPEGVRIQQIYRGIVPFVSLQLVGLLVVIMFPALALWLP
                     HVMLD"
     gene            complement(18278..19066)
                     /locus_tag="VGB88_08365"
     CDS             complement(18278..19066)
                     /locus_tag="VGB88_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647497.1"
                     /translation="MVDIEALKDSLTRREQGPDRRPLWTVPAAHAEDMARAAEEVLEC
                     QDALARGGANLVGEVLRGQGAFTEWSHYPKGDVYDRQTHAQYYYHAHPSSLRGGEHGH
                     FHTFVRAKGMPDGIEPAPLPAAVERPLGEDALSHLVGISMDKAGRSLRLFTTNRWVTG
                     ESWYAAADVIAMLDRFRITHAVPSWPANRWVGAMLRLFRPQVEWLLGERDEAVRLWQL
                     GNAGAETSVYEDRGLEVTSVLDISVDEQVRLVRAAAAGQTVSQA"
     gene            complement(19081..19950)
                     /locus_tag="VGB88_08370"
     CDS             complement(19081..19950)
                     /locus_tag="VGB88_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9647498.1"
                     /translation="MAMSWCRPAVVMVALAAAAGLGRARAEPAEAPEVVWIPPGPFIA
                     GSDPAEREAAYRLDAAAYGHDATRRQGWYDRERARGPVELAGYAITRTPITNRAYAAF
                     VAATGHPAPAVDRETWDGYRLAHPYERTLGYQWLEGVPPAGKADHPVVLVSHADALAY
                     AGWLSKATGLTWRLPSELEWEKAARGTDGRRFPWGEAFDPARLNSHDRGPFATMAAGS
                     FTGGASPFGVLDAAGQVFEWTSTEAGPGRYLVKGGSWDDKGCGVCRPAARHARPGGLK
                     HILIGFRLVRAPD"
     gene            20090..20341
                     /locus_tag="VGB88_08375"
     CDS             20090..20341
                     /locus_tag="VGB88_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647499.1"
                     /translation="MDKVKSTRSRLLATTVLPMAIVAAIGAASPAYAACQACKPCAAA
                     ACSACSPCKASACAACNPCNPCAAAGCKACNPCNPCKPD"
     gene            20548..20931
                     /locus_tag="VGB88_08380"
     CDS             20548..20931
                     /locus_tag="VGB88_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9647500.1"
                     /translation="MIQRILAFAAAAGIAIAAAAPAGAADLEAGEKVFKKCKACHTIE
                     ADGKNKIGPNLHGVLGREAGTMEGFKYSKAMAEAGITWDEDRLRQYLADPKAYIEGNK
                     MAFPGLKDEADRENVIAYIKQESGE"
     gene            21052..21624
                     /locus_tag="VGB88_08385"
     CDS             21052..21624
                     /locus_tag="VGB88_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX9647501.1"
                     /translation="MDLTGEYRIPAPRAEVWQALNDAEVLRACIPGAEALDKLSDTEF
                     QATVKAKVGPVAARFSGKVTLSDLVPPEHYRISGEGTGGAAGFAKGGAEVTLAEADDG
                     GTVLSYRADVQVGGKLAQIGSRLVAGTARKMADDFFGRFAERLGGARAEAPAEAAAVA
                     PPTERRALPPAIWIAALILLVVILLVIVGA"
BASE COUNT         3050 a         7954 c         7844 g         3054 t
ORIGIN      
        1 cggcgagggt caggtgattg tcgatgccct cgttgagtgc gaagcgctgg cacagccggt
       61 aggccgcggc caggtcctcg cgcgcttgcc ggacctcctc catccgggga tcctcgaccg
      121 tcgccaccgc cattgcctaa aacccggttt cacaaattgc agttgcccgc ctcatccttc
      181 gagacgggcc ttcggccctc ctcaggatga ggctatgaat aaacacaaac ctcatcctga
      241 ggagcccgcg cagcgggcgt ctcgaaggat gaggtttgcg gctcatgttt cggcgatagt
      301 tggtattaga acacgatgag ctggcgaacg gtatggcccg aggccaggcg gtcgaacgcc
      361 tcgttgacct cgtcgagccc gacccgctcg ctcatcagcc ggtcgaccgg cagccggcct
      421 tgctgataga gcgcgatgta gttggggatg tcgcgctggg gcacgcagct gcccagatag
      481 ctgcccttga tggtccgctc ctcgccgacc aggttgacgt gctggaccga gaagctgtgg
      541 tcggggtgcg acaggccaga gctcaccgtc tcgccgccgc gccgtgtgat ccggtaggcg
      601 agctccatgg ccttgacgtt gccgaccatc tcgaacgcgt agtcggcgcc cccgcccgtc
      661 gccgcgcgca ccgcctcgac cacgtcgtcg gcagcggcgt tgaaggtatc gttggcgccg
      721 agctggcggg cgagcgcgag cttgtcgtcc aacaggtcga tggcgacgat gcggcgcgcg
      781 ccggccatgc gcgcgcccaa cagcgcgttg agcccgaccc cgcccaagcc gaccaccgcc
      841 accgaggcgc cggggcgcag gcgcgcggtg ttgaccaccg cgccgacgcc ggtgatcacc
      901 gcgcagccga acagcgccgc ctcctcgagc cccagctccc ggtcgacctt gaccagggag
      961 cggcgcgaga cggtggcgta ctcggcgaag caggatacgc cgacctggtg gttgagcgcc
     1021 gccgcgccct tgtgcagccg gcggccgccg gcgagcaggg tgccggcggc gttcgccgcc
     1081 gcgccgggct cgcacagggc ggggcgcccc tcggcgcacg gcacgcagtg gccgcaggag
     1141 gggacgaaca cgaacaccac gtgatcgccc ggcttgaggt cggcgacgcc ggcgccgacc
     1201 tccgcgacca cgcctgaggc ctcgtgcccc agcaccatcg gcagcggccg cgggcgggcg
     1261 ccgttgatca ccgacaggtc cgagtggcag agcccggcgg cggcgacctt gaccagcacc
     1321 tcgcccgcgc cggggccgtc gagatcgagc tcctcgatct ggacggggcg gctctgcgcg
     1381 tagggctcgg gctgtcccat ctcgtacaga acggcggcct tgatcttcat gatgcggcga
     1441 ggtcccaagc ttccggagcg ggcgggctac cagggcagcg cggagccgtc atagcgccgg
     1501 aacttgccgc tgtcgtcaag cttgagactg tccacgacgc gcttgagtcc ggcggcgctc
     1561 cggccagggc tgaggctcgc gcccgcgccg cccatgtcgg tgcgcaccca gcccgggtgc
     1621 aacgcgacca cgatcacccg ccggcgcctg aggtcggccg ccagcgagcg caccgccatg
     1681 ttcagcgccg ccttcgagga gcggtagagg tagtagccgc cgcccttatt ctcggccatc
     1741 gagcccatgc tgctcgacat catcgcgatc acgcgccggt ccgaggcggc gacgttgtcg
     1801 gccagcgtct ccgccatcgc caccggcgcg agcaggttga cccgcagcac ctcggcccag
     1861 ccggcgtagt cgatgtcgcc cagcgactgg ccgtcccgcg ggcctaacat acccgcattg
     1921 ttgaacagca cgtcgatcgg cagcccctcg agccaggccg cgaactcgcg gatcgcgcgc
     1981 ggctcggcga cgtcgagcgc ccgcacctcg accccctcat gcgccttcgc cagcgcacgc
     2041 agctcgtcgg ccttgcccgg gtggcggcag gtggcgagca ccagccaatt gtccgcggca
     2101 tacgccttgg ccagcgcgag gccgatgccc cggttggcgc cggtgatcag gatcgtcggc
     2161 atcgattgcc tagaacgcga ccgcgtcgcc acccttgagc gcgagcatcg cgcgcgcctc
     2221 gtcgggagtg gcgatgtcga gcgacaggcc gtccagcacc tggcgcatcc gggtgacctg
     2281 ggcggcgttc gactcggcga gccgtccggg gcccagccac agcgagtcct cgagcccgac
     2341 gcgcacgttc gcgcccatcg ccgcgccgat ggtggcgagc gggatctggt tgcgcccggc
     2401 gccgaggatc gaccacaagt aggagtcgcc gaacagcctg tccgccgtcc ggcgcatatg
     2461 catcaggtcc tcgggatggg tgccgatgcc gcccaggatg ccgaacacgc tttgcacgaa
     2521 cagcggcggc tcgaccaggc cgcggtcgag gaagtgggca agattgtaca ggtgggcgat
     2581 gtcgtagcac tcgaactcga agcgggtgcc gttgccgcgg cagctcgaca ggatgaactc
     2641 gatgtcggca taggtgttct tgaacaccat gtcgcggctg ttctcaagat gccggcgctc
     2701 ccagtcgtgg cggaactcgt tgaagcgctt gagcatcggg aacaggccga agttcatcga
     2761 gcccatgttg agcgaggcga cctcgggctt gaacgtggcc gcgggcgtga ggcgttcctg
     2821 cacggtcatg gtcggcgcgc cgccggtggt gatgttgatc acggccttgg tcgcctgctt
     2881 gatgcgcggc aggaagcggt cgaacacctt ggggtcctgg gtcgggtggc cgtcgtcggg
     2941 gtcgcgcgcg tgcaggtgca ggatcgccgc gcccgcctcg gcggcggcga ccgcggcctc
     3001 ggcgatctcg tccggggtca ccggcaaatg cggcgacatc gacggcgtgt ggatgctgcc
     3061 ggtcaccgcg caggtgatga tgaccttgcg gccgttggcc attgcctctc ctcctccgcg
     3121 ctcagcggcc gatggtgcgc gccgcgcgca gcttggccgc gaccaggtcc gcgagccgcc
     3181 ggtcgcgcca gcgcgcgcgc tcggcgagct tgtccgcggg cagcgccttg cgccggtcgg
     3241 cctcgacccg gtcgaggccc gcgcccacca tcaggcgcgg ctccggatct tcgctggcga
     3301 ggccgtggta catgcgcgcg aaccgctcgc aatagtcgcg cacgccgccg ggcgcgttca
     3361 agtcgatggt ctcgaacggg cccatgaacg cccagcgcag gccgatgccg tcggcgatcg
     3421 cggtatcgac gtcggcggcg tcgcacaccc cgtcctcgac cagcttgaac gcctcgccca
     3481 gcagcacccc ctgcatgcgg ttggcgacga agccgtcgac ctcgcgcttg agcgtgatcg
     3541 gcgcctggcc gaccgcgccc atcagcgcag ccgtgcgcgc gaccgcgcct ggatcggtcc
     3601 acggcgccgg cacgatctcg accagcggca ccaggtgcgg cgggttgatc gggtgcgcga
     3661 ccaggcagcg cgcgcgcccg ggaagctccg cggtgaagcg cgaggcgcgg atgcccgagg
     3721 tcgagctcgc gagcacggca tgcggcgcgg ccaggcggtc gagctcggcg aacagcgcgc
     3781 gcttgacctc gaccttctcg ggcccgttct cctggacgtg gccggcgccg tcgagcgccg
     3841 cggcgagcgt cgccgcggcg tggatgcgcg cctgcacggc cgcgggcgcg tcgccgccaa
     3901 gcagcccggc ctcggcaagc tcaggcagcg ccgcagcgat tgcctcaagc gcaccggcaa
     3961 ccaccccgtc ggcgggatcg tagagcgcca cctcgtggcc ggcgcgggcg aacacgattg
     4021 cccacgcgcg cccgatcagc cccgcgccca ccaccgcgat cctgtcagcc atcgcccgct
     4081 cctgccgcgc cgcggctcag gtgatgtcgg tcgagtccgc gcacaccgcg atcgcctggc
     4141 cggagatgtt gcgcgcctgg ggcgagctca ggaacaggat cgtgccggcg atgtcgtcgg
     4201 cggtgacgaa ccggccgagc gaggcatggg cggtgagcgc ggcgcggatc tcgtccgggc
     4261 tgacgccctg cgccgccgcc ttggcctcga tcacccgctc gatgcgcggc ccggcgaccg
     4321 gtccgggctg gaccgcgttg acccggatgc cgaacgggcc gagctcgcgc gacagcgact
     4381 tcgacagccc gaccaccgcc catttcgacg ccgagtaggg cgtccggaag gcgtagccga
     4441 acttgcccgc gaccgacgac aggttgacca tcaccgcctc gccgtgctcg gcggcggcgg
     4501 ccttgagcag cggcaccgcg cgccggcagc acaggtacat gctggtcagg ttgacctcga
     4561 tcgtgcgccg ccactcggcc gcgtcgagcg actcgatcgg gccggtcggg ccggcgatgc
     4621 cggcgttgtt gaccatcacg tcgaggccgc cgagcgcgtc ccgggcggcg tcgaaccagc
     4681 gcccgacctg gccgtcgtcg gcgacgtcgg cgggcacccc ggcgacgccc gcgacctcgg
     4741 ccaggcacgc ggcgagcgcc tgctcgtcga cgtcgcagat atgcaccttg gccccggcgg
     4801 cggcgaacgc ggccgctgtg gcgcggccga ttcccgccgc gccggcgctg atcatgaccc
     4861 gaagacccgg cctcaaggcc ggccccgcga ccgcctcggc cactctcccc tccccgtgcc
     4921 ggctgtgccc cggcgggggc actggtacgc gatcaccccg ccggtggcaa cgcccgagtc
     4981 agcgcaccgc gtctgccggg gcccgcaccc gccctctgcg ctcgggcacg agctttagcc
     5041 gcggccggcc gcgcagcgcc agggcggcgg cgtgcagcac gtcggcatag ccctcgcccg
     5101 gcgccaccga gcggccgagg ccgagcttgg cggcggcctc gaacggggtc aggaccagca
     5161 agtgactcgc cggcagcggg cgccgggcga gcgccgcctt ctcgtcctcg gcggtcaccg
     5221 cgatcaggtc gatgccgtcg ccctggcgaa agccgagccg cttggcgtaa tcgatggcgt
     5281 ggcggaactc ctgctcgatc tgctcggcga ccgcatccgc aggcgtgtcg ggatcgaggc
     5341 tgcgggtgag cctggagaag gcgatgcgcc cgtcggccgc gatcacctgg cggaagccgc
     5401 ctgtgcgctc gcggctgacc atcacctgcc aggcgctgcg cgcctcgccc aagggcgcgg
     5461 ccaggcgctc gagcatcact gccgcctcga ccggcagcag caccaccgcg ccgacccggt
     5521 tgggcacgcc gtcgaggaac gccatccagc cgtcatagcc cgccgacggc gccacgcccg
     5581 cgatcaggaa ctcgcggtcg cggcggcctt cgcgcgcgcg cccctcggcg agtgcgccgg
     5641 tggcgcggaa gccggcgaag gcgcgcgcca tgcgccgccg caacaggctg cccgtctgcc
     5701 atgccgcgac cggcggcagg gcagcgcgct gcaacgagat ctcgagcacg tcgaccatca
     5761 ccgtcaccgg ccagcgcggc gcggccgcga gcatcgagcg cagctcgccg gcggcatcgg
     5821 gcgacggcgc gtaagcccgc tcgatcacgt cccggccgcg gcaggcgcac agcaccgcgc
     5881 cctcgttgcc gaccaccagc acgaaccgcc ggccgccgcc gaagagggaa ctgtttgcca
     5941 ggctaaccaa gagcgccaaa actgttgtag atcgggccga gcaccgccac cgccacccag
     6001 gcgagcaggg ctcccaggac cgccgtcacc gccggttcca gggcggcaat cagcgccgag
     6061 acagcgtcgt cgacctcgcg gtcgtagaaa taggtgacgt tgtcgagcgc ctcgagcagc
     6121 gcgccggctt cctcgccgac cccgaccatg cgcaccacca ggcttgggaa ctcgccggtc
     6181 gactcaaggg cgcgggcgag cgagtggccg gaggcgacgc gctcggtcgc ggcggcgacc
     6241 gcggcggcga tcctcaggtt gccgaccacg ccgcgcgcgg tctccaggct cgccagcagc
     6301 ggcacgccgc cgcgcagcag gaccgcgaaa tagtggctga acctggacag cgcgagccgg
     6361 cgcagcaccg ggcccagcag cggcaggcgc agcttggcgg cgtcgatcgc gaccgcggcg
     6421 gcgggcgagg cccggcgcgc cagcgccgcc gccgcccaca gcgccaccgg cacggcgacg
     6481 agcgcgggcc aggcggcgat gaagaactcc gagacggcga gcagcgccac ggtcaggaac
     6541 ggcaggccga ccccgacctc gcgcagatag ccgaccaccg acggcaccac ataacccagc
     6601 atcagcgcga acaccgccgc cagcagcagc ccgatcacca gcggatagcg gagcgcgcgc
     6661 cggatgcgct ggcgcagctc cgccagccag cgcaggtggc gcgcgaggtc ggcgaaggag
     6721 tcggctaggt tgccggtctc ctcgccggcg cggaccaggc cgacgaactc gccgctgaag
     6781 gcgcgcgggt ggcgggcgaa ggcgcccgac agcatctggc cgtcgcgcac gtcgttgtgc
     6841 gccgccgaca gcacgtcgcg cagctcaggc gagccggtga cgtcgcggat gccggagagg
     6901 cagtcgagca ccggcacgcc cgcgcgcagc agctgcccca tatggatgca cagctgcatc
     6961 atgtcggtga tccggacgcg ccgcccgagc agcgcgccga gccggcccgc gcgcagcggc
     7021 cgctcggaca acaggtcgag gctgaggccg ccgagccggc tttcgaggtc gagcgcattc
     7081 gccgccgaca tggtgccgcg caccacgcgc ccgcggccgt ccatcgcccg ataggcgtag
     7141 tcgggcacgg ctggccccta acgcctgccg gtcaggttca ccgcgcgcgc cactgcctcg
     7201 acgctggtga gcccggccag caccttgtcg attccgtcct cggcgatcgg ccggaagcca
     7261 ctcctggacg cgctggcgag catcgccgtg cgcgtgccgc cgctcgcgat cagctcgtcc
     7321 agatcatcgg tgaccggcag gatctcgacg atcgcggtcc gcccgcgata gccgagctgg
     7381 tcgcaggccg cgcaccccac cggatcgtgg accgtgggcg gcgccgccgg atcgccgccg
     7441 agcagctcgc attcgtcggc gcgtgccggg cgcgggcggc ggcattcggg acacaggatg
     7501 cgcaccaggc gctgggcgac gcagcagatg atctggccgg cgagcaggtg gcgcgggacg
     7561 ccgatgtcga tcaggcgcgc gatcacgccg accgcgtcgt tggaatggag ggtggtgaac
     7621 acctgatggc cggtcatcgc cgcgcgcagc gccatcaggc cggtgtcctg gtcgcgcacc
     7681 tcgccgatga acaggatgtc ggggtcctgg cgcagcatcg agcggatgcc ctcggcgaag
     7741 ttcatcgccg tgtcctcgcg cacgttcgcc tggcggatca gcggcagctg gtactcgacc
     7801 gggtcctcca gggtcatgat gttgacgtcg gtcgcgttca ggtagcgcag gatcgagtac
     7861 agggtcgtgg tcttgccggc gccggtcggg cccgacacga acacgatgcc ctcgggccgg
     7921 cgcaacagcc gcttgagcag cgcgacgttg gcctcgccga agccgagctc ctcgagcggc
     7981 agcagcgagc gcgccttgtc gaggatgcgc atgacgatgt tctcgccgtg gaccgtcggc
     8041 tggctggcga cgcggaagtc gacctcgcgg ccgctcatca agtaactcag gcggccgttc
     8101 tgggggttgc gcgaatcggc gatgtcgagc cccgagatta ccttgatgcg caccgcgatc
     8161 gccggccagt agtccttgtg gaacgcgcgc accggtatca tcacgccgtc gatccggtag
     8221 cgcagcctga ggaacgcgcc ctcgggctcg aaatggatgt cggaggcgcc gcgcttgacc
     8281 gcatcgacca ggatcgcgtt gaccaggcgc acggtcgggt tgacgtagcc ctcctgctcg
     8341 ccgcccgagg cctcgcgctc gccggtctcg agctcgcgca ggatgccggc gatcgacatc
     8401 tggtagccgt agctctggtc gatcgccgcc tgcaggtcga cgtcggtcga gaccagcggc
     8461 tcgatgcgca gccgggccgg caggtgatgg cgcacctggt cgatggccat gacgttgtag
     8521 acgtccgcca tcgccagcag cagggtgtcg ccgaccaccg ccaccggcag caccttgtgg
     8581 cgcagcgcaa tctccttggg caccagctgg acgacatcgg gatcgaccac ggtggtgccg
     8641 gcatcgaagc gcgcgcgccc ggtcgactcg gcgatcacct ggctgagcgc gccctcggtg
     8701 atgaagccga agtcgaccag gatctcgccg agcagcttgc ccgagcgctt ctgctcgagc
     8761 agggcgacct cgagctggtc gtcggacaag aggcgcagct cgcgcaggcg ctcgccgagg
     8821 cgcaggctgg cggcggggtc cgcgccctcg gctcgtgcct cggcgccgcg ctcgcccgcg
     8881 gccgctccga gccgccgcca ggtcgcgccg gcgccggcgc tggccggcgg cgcgggcggc
     8941 gaagggccct tgaaatgaag atcagccggc actcaagcga ccctcgcggc catgccgccc
     9001 gtcttaacat gccctgccac caccagaaag cccgctcgcg gcggccggcg ggcggcttcg
     9061 cggccggcgc ggcgcgctag aacgcgaacc ccatcacgca ggcgggcgca ccaccggcgg
     9121 cagtgaggta ctggccgtcg cgctcgcacg gcgtcgcgcc cggcccggcg ccgtcgcccg
     9181 cctggaccag gccggtgttc ggccggccgt cgtcgatctt gaggtccagc agataggcct
     9241 cgtcggccgg cagcagcgcg accgtggtga gcgcgttctc gtccggcgcg cccagccgcc
     9301 agtagttgcc gcgcagcgtg cagtcggcgc acagctcggc gccgggcgtg gcggcgttga
     9361 ccgcattcca ggcgctgccc ggcacgcgtc cgaccgggac tcccaccccc ggcaccgggg
     9421 cgatgccggc aatgccgccg atcatgtcgg cggcggccag gtgctgccag tactcgagcg
     9481 actcgccgtc attgccgagg ccggcgatgc ggccgttgcc gtcgccgtcg ctctgcgccg
     9541 agctgccgtc ggacctcgcc ggcagcaggc tggaggcgcg gctgaagtcg cccggaatct
     9601 gcccgtacct gtcgatgaag ccattgttgg cggcgcggaa cgagtcgatc tgggtgatca
     9661 gcgccttgga ctgggcgttg cggatcagct cctggccctt gaggatgccg ccgatcagga
     9721 gcccgatgat gatcagcacg atcgacagct cgacgagcgt gaagccccgt tgcgcgcgca
     9781 tacgtccccc accaccggcg gcatgcggcc cgtacggtcg cgcgccaggc gaggcggcgc
     9841 gaccggcctg cggattaagt tagaggcggt gtggccagcg gcaacccaca accggaacgg
     9901 gtcggcagcg ggcggcccgc tgctgcccgc gagcggtagc ggcacaacca tctgtgcggc
     9961 aaccagcggc gctcaccacc agcccggcca gggcgcgcgc cacagcacgg tcgcgaacag
    10021 ggcggcggcg agcgcggggc caaacgggaa cgcctcgccg ccgcgccgcc gccaagccag
    10081 gcccgaagcc acgccgaggg cgccagcgcc ggccaggtac cacggcagcg cggcgaggcc
    10141 gacccaggcg cccgccaccg ccaacagctt gacgtcgccg aggccgagcg cctcgcggcc
    10201 gcgtgcccgc gcgcccagcc agcgcagcgc cgccgccagc gccagcgccg cggccaggcc
    10261 gccgatcgtg tccgaccagg ggcggccgag cacggcggcc caggcgaggc cgagcggcag
    10321 cagacagacc agcaccggat cgggaatcac cagccggtcg aggtccgcgg cggcggcgat
    10381 caccaggccg agggcgagcg cgcacgccag cagcgcctcc ggcgtgacac cccaggcgag
    10441 cacggcgagc acgaacagcg ccgcggtggc gagctcgatc agcggatagc gcaccgggac
    10501 cggcgcggcg cagtagcggc agcgcccgcc gagcgcgagc cacgagacca ccggcagcag
    10561 gtcggcgagg gcgagcgcgt ggccgcagcg cgggcagcgc gagcgcccga gcaccacgcc
    10621 ctcgccgcgc ggcagccggt aggcggcgag cgcggcgaag ctgccgaagg cgaggccggc
    10681 gactccggcc agcaccgcga ccgcggcaag cggcatcgac gtgctcaccg cggcgcctcg
    10741 ccgagcgggt cgcgggcgcg cgcatagacc agcgcggcga gcacgatgat cgcggccagg
    10801 aaccagaaca gtgcgccata ggccgccggc ggatgcgcgc cggcctccgc cggccaggca
    10861 tcgacgatcc agccgctggc catctggacc aggccgacgc cgccgaagtt gaacaggttg
    10921 gccgcggtca gcccgcgccc gaccatgtgg tccgggaaca ggccgcgcac gtggctgatc
    10981 agcagcaccg agtaggcgcc gagcaggccg gcgagcgcca gcagcgccgc gaccgcgccg
    11041 agcccgagcg gcaggccgag gccgagcacg gcatagatcg ccgccacccc gccggcgcct
    11101 gcgagtgccg ggcgcttgta gccgcccagc cgcggcgcca gccagccgta gctgattgag
    11161 cccgccatca gcatcaccgc catgcccatc atcaccatgc cggcgtcgac cgcctcgagg
    11221 ccgtggacgt cggtcaaata cggcgcgccc cacagcccga gcaccgccac caccgaggcg
    11281 taagcgaccg cgttcagcgg cagcagccgg tgcagctcgc gattgcccag cacggtggcg
    11341 acgccgcgcg cggcctcggc cacggattcg cgcccgcggt cgtggaaggc gcgcccgggc
    11401 ggggcgtcgc gcaccaccag ccagaccagc gcggcgacgg cgagggtgag cgcggcggcg
    11461 gcgaggaacg cgccgcgcca gccgagcgcc gccgccgcat aggcgagcgg cgcggtcgag
    11521 accagggcgc cgaggccgcc gaccgacagc atcagcccgg ccagggtcga gaaccggcgc
    11581 gcctcgaacc agcgcgagaa caccaccagc gcgcccatca gcccggccgc gcagccgatc
    11641 ccgatcaggc cgcgcccgag ggtgagcacg ccggcgtcgg gcgccgccgc gaacaccgcc
    11701 gcgccgagcg ccgcgaccac cagcagcacg gcgctgaccc ggcgcgggcc gaaccggtcg
    11761 agcgccacgc cgaccggcac ctgggccacg gcgaaggcca ggaaatagct gccgccgagg
    11821 gtgccgagcg cggacgccga cagcccgagc gtggccatca gctcgggccc gatgaccccg
    11881 accgaggtgc ggtagaactg gctcagcaca taggccgcgg ccagcaccgc caccaccgca
    11941 accgcggccc gcgcgccgcc cgtgcccggc gcgcggcggc gctgggcgtg cgggacggtg
    12001 gcgtggcgcg gggatttcat cggggcggtg cgggtcggag cggttggcaa gcgggcggcg
    12061 gcagcgaggg ttattagctt gaccgcggcg cgcgcgacaa gctaccaacc gcccgatccg
    12121 gcccaagccg aggtccgcgc catgcccgtt gccggcgagc cgcttgccgc ctgtcccgtg
    12181 acgttctccg ccttctcggt gtgcgaccat cacccggcgc tggcgcgcac gctcgaccag
    12241 ttctatcgcg agctcctggg ccatgccgtg ctcgccgagg agctcggcta cgacgcctac
    12301 tgggtcgccg agcaccactt ccacgaatac ggcgtggtgc ccaacccggc gccgttcctt
    12361 gccgccatcg cccaggccac gcggcgcatc cggatcggcc cggcggtctc ggtgctgccg
    12421 ttccacgacc cggtgcaggt ggccgaggac tacgccgtcg tcgaccagct ctccggcggg
    12481 cggctcaaca tgggggtggg ctcgggctac ctgccgcacg agttcgcggg cttcggcgtc
    12541 ggcggcgaca ctaagcgcgc gcgcttcgac gaggcgctgg tcctgatcag gcggctgtgg
    12601 gcgggcgaga cggtgacctt cgagggcgcg ttccatcgcg tccacggggt cgcgctcaac
    12661 gtgctgcccg tccagcggcc ggcgccgccc ttgctggtcg cggcgctgag gcccgaggcg
    12721 tgctaccaca tcggccgcca cggcctcaac atgatgacca tcccctatgc cgcggtcgac
    12781 cggatggacg ggatcggcga catggtcgcc gacttccggc gcggctggca ggagacgcaa
    12841 ggcacgccgc cgccggtgcc gctgtgcgcg ctgcacgccc acgtcgcggc ctcggacgag
    12901 gcggcgcggg cgaacgccga ggcggcgttc aaccagtaca cgcgcacccg cctgtacgcc
    12961 aagagcaaga aggactacga cgacatcctg gccgccgagc tcggcttgtt cggctcggtc
    13021 gagacggtgg tcggccagct caggcgcctg cacgcgatgg gtgcccgcca ggtgatgctg
    13081 ctgcaggact tcggcgcgct ggccgcgggc aaggtgcgcg agtcgatgcg ccggttcgcg
    13141 accgaggtcg cgccgaggct cgccgagcgc gtgcgcgccg acgccgcggc ctgagcgcgg
    13201 cacgggaggc ggtcgatgct cgagctgagg cacgcccgtt ggattgccgt cgccgcggcg
    13261 ctcgctgcgc cggcgcacgc ggccgagatc gagctcgtcg ccacccactt cgtctccgcc
    13321 cagcacggcg tccaggtcga catgctcggg ccgtgggcgg ccgagctcga gcgccgcacc
    13381 ggcgggcgcg tgcacgtgac catccatccg ggcggcagcg cgctcggcaa catcaacaag
    13441 cagtacgacc aggtgcgcgc cggcgtcacc gacatcgcct tcggcctcaa cggcatcccg
    13501 cgcgggcgct tcccgcgcac ctcgatcatg gcgctgccgt tcatgaccgc gagcgccgac
    13561 gcggcgacgc gcgcgctgtg ggacgtgttc cccgaatacc tggccgagga gtatccgggc
    13621 gtccacgtgc tcgcgctcac cgcccacaac ggcggcatga tccataccgt ggacaagaag
    13681 gtgaccacgg ccgaggacct ggccgggctg cggatccgcg cgccgagccc ggaggtcacc
    13741 tcgatgctcg aatatctcgg cgcctcgccg gtcggcatgc cgccgggcga ggtctacgag
    13801 agcctgcagc gcggcgtgct cgacggcgcc atcttcccgt gggacccggt caagtcgatg
    13861 cacatcgccg aggtggtgcg ctaccacctg gagatcggct gctacacggt gccgttctac
    13921 ctggtcatca acgagcgccg ctggcagggc ctgccggccg aggtgcgcca ggcgatcgac
    13981 gacatcagcg ggccggcgct gatcgagcag ttcggcggct ggtgggacaa gtgggacgcc
    14041 gccggccgcg ccgcggcggt cgagcgcggc aacgtcatct cgaccttgtc cgacgccgag
    14101 cgcgcggcgt ggcggctgcg gctcgagccg atgatcgagg cctggctcgc cggcctcgag
    14161 gaccagggcg tcgacaacgc ccgcgagatc ttcgacgcga tgcgcgcgcg catcgtggtg
    14221 cacgaggcgg ctcactgagc gagcccgcgg tcgagcgcgc ggcgcgcctc gcccgcggcc
    14281 ttgcggttgc cggcatgctg gcgctggtcg cggcgatgct gctgaccgtg gtcgacgtgg
    14341 cgctgcgccg gccgttcgac atcgccatca agggcaccgt cgacctgacc cagctgttgg
    14401 tgatggccgc cgcctggctc gccatcccgt atgcgttcct gaccgacggc cacgtctcgg
    14461 tgccgctgct ggtcgaccgg ctgccggcgc gcctgcgcgc gggcctggcg gtgctcgccg
    14521 cgctgctcgg cgcggcgctg ctcgccggca tcgcctggta cggcaccgcg caggccaggc
    14581 tcgagcacgg ctacggcgag atctcgctca ccctcggcat cgccaagcac tggtactggc
    14641 tgccgctgat cgccggcgcg gcgctgtcgg tcgcggcgtg tgccgtgcgc gcgctggccc
    14701 aggcgtggcg cccgcgcccg ggccgcggct gagatgtccg accccgcgat cggcgtcgcc
    14761 ggcttcgcgg tgctgctggt cctgatcgcc ctcggcgtac cggtggcggt ggcgatgggc
    14821 gcggtcgggg tggtcggctt cgcggtgctc ggcggctggg acggcgccgc cttcgtgctc
    14881 ggctcggtgc cgttccaggc ggtctacccc tactcgctcg cggtagtgcc gctgttcatc
    14941 ctgatgggcg ccttcgccag ccgcgccggg ctgtcgcgcg cactctacga cggcgtcgat
    15001 tcgctggtcg gccactggcc gggcggcctc gccatggcca ccgtcggcgc ctgcgcgggc
    15061 ttcggcgcga tctgcggctc gagcctcgcc accgtcgcca ccatggcccg cgtggcgctg
    15121 cccgagatgc gccggcacgg ctacgacgac cgcctggcgg cgggcgcggt cgccgccggc
    15181 ggcacgctcg gcgtgctgat cccgccgtcg atcctgctgg tggtctacgc gctgctcacc
    15241 gagcagtcga tcgcgcggct gctggtcgcg gcgctggtgc cggggctgct cgcgaccgcg
    15301 ctctacatgg ccgcggtcgc ggtccaggtg cgcgttcgcc cgcacctcgc cccggccagg
    15361 gaacggcgcc cgcccgcccg gcggctcgcc gccgcgctcg gcatgtggca ggtggcgctg
    15421 ctgttcgcgg cgatcatcgg cggcatctat ctgggcgtgt tctcggtcac cgaggcggcg
    15481 gcggtgggcg cattcggggc ggtcgggttc gcgctcgcgg ccggcgtcgg gcgggccagc
    15541 gtggtcgacg gcctggccga ggccgcctcg accaccgcca tgatcttcct gatcctggtc
    15601 ggcgccgggc tgttcaactt cttcgtcgac gcctcctcgg tgccgatctg gctggccgag
    15661 cggatcgcgg cgctggcctg gccgccgacc gcggtggtgg tggcgctgat gctgttctac
    15721 ctggtgctcg gctgctttat ggactcgctg tcgatgatgc tgctgacggt gccgttcgtc
    15781 tttccggtgg tcctggcgat gggcatcgac ccgatctggt tcggcgtctt gctggtgacc
    15841 gcggtcgagg tcgggctgat cacgccgccg atcggcctca acctgttcgt gatcaagagc
    15901 gtcgagccga cgctcggcct cggcgtggtc atgcgcggca gcatcccgtt cgtcgccgcc
    15961 gacctggtac ggatcgcgct gctggtcgcg ctgccgcagc tgagcctgtg gctgccgggg
    16021 cggatggggt agttcggcaa cgccgcgcgg cgcccggcgg cccgggcttt ggcccgactt
    16081 ttcctgttgt cacggcgcgc acgtgctggg atcatgcgcc gccgcggtcg gaccggcgac
    16141 cgtgtgccga cctgccgggg gacggacgcg gggggcgtgg cggccgggcg gcgcccgaga
    16201 cgaggcgaca ggcggagagg gagggatgcg acagctgata gcggtcagcg aagtgctgga
    16261 tcgcatcatt gcgaagatcg gcaagctggc tgcgtggctg ctcatcccga tggtggggat
    16321 catcgtcatt gacgtggtca cccgcaagtt cggcctgctg agcaagctgc gcgaggccct
    16381 ggaggcgagc ggggcgatcg aagcggcgca gttcgtccag tcctacatga cctcgaccaa
    16441 gttccaggag ctcgagtggc acctgcacgc cgcgctgttc ctgctctgcc tcggcttcgg
    16501 ctatgtccgc aactcgcatg tccgcatcga gctggtgcgc gagcgcttcg gcgtgcgcac
    16561 gcgcgcctgg ctcgagctgg tcggttgcat gatcttcctg gtgccctacg ccggcctggt
    16621 gctctatttc ggcatcgact tctcgcagcg atcgttcgag atgagcgagg tgtcatcggc
    16681 gctgaccggg ctcagccacc gctgggtcat caaggcgttc gtgccgctcg gcatggtgct
    16741 gctgctgatg gcgacggccg cggtggtgct gcgcaacgtg atcttcctgt tcggcagcgg
    16801 cgcgcttcag ggacgcgccc tcggcgaggc gatggagctg cactcggcga tgcaggacct
    16861 ggtcgcgtcg acggtgcgcg aggctgaaga gatcctggcg gtcgagaaga aggagccggc
    16921 gtcatgacct tcgtcgattt cctgccggtg ttcatgttct tcagcctcgc gatattgctg
    16981 ttcagcggct acccggtggc ctgggtgctc ggcggcatcg gcgtcagctt cggcgccatc
    17041 ggcatgctct tcggcgtgtt ctcgttcgtc gaatacttca acatcatctc gcgcatctgg
    17101 ggcacggcgg cggagaacct gatcctggtc gcggtgccga tgttcatctt catgggcacc
    17161 atgctcgagc gctcgggtgt ggccaacgac ctgctgtacg ccctgcaggt gctgctgcgc
    17221 cggacaccgg ggggtctcgc gctgtcggtc acgatcatgg gcacgatcat ggccgcgacc
    17281 accggcatca tcggcgcctc ggtggtgatg atgaccttgc tcgcgctgcc agtgatgctc
    17341 gatcgcggct acgacatccc gctcgccacc ggcaccatcg ccgcctcggg cacgctcggc
    17401 atcctgattc cgccgagcat catgctggtg atcatggccg atctgctgtc gatctcggtc
    17461 ggcaccctgt tcctggcggc gatcgtcccc ggactgatcc tgtcggggct gtatctcgtc
    17521 tatatctaca tcgcgacccg catcaagccg tcgctggcgc cgcccttgcc ggacgagatg
    17581 gggccggaca cgcgcgctca gctggtgacc atggtgctgc gcagcttctt cccgccgatc
    17641 atgttgatcg tgttcgtgct cggctcgatc ttcgccggct gggcgacgcc gaccgaggcc
    17701 gcgggcgtcg gcgcgctcgg cgccgtcctg ctcgcggtat tcaaccgcaa gttcagtttc
    17761 accgtgctca aggacgtcag cgagcgctcg gcgctgacca tcgcgatgat cttcggcatc
    17821 ttcatcggcg ccacctcgtt ctcctacgtc taccgctcgc tcggcggcga cgacctggtc
    17881 cacgctttcg tcgaccatgt cggcttcggc tcgtggggcc tgttgatcct gatgatggtg
    17941 ctggtgttcc tgctcggctt cttcttcgac tggatcgaga tcacgctgat cgtgctgccg
    18001 ctgttcgcgc cgatcatgcg caccatggac ttcggcacgc acatcccgtt ccaggtcgag
    18061 gtgatcccgt ggatcgcgat cctgctggcg gtcaacctgc agacctcgtt cctcacgccg
    18121 ccgttcgggt tcgcgctgtt ctacatgaag ggcgtcgcgc ccgagggcgt gaggatccag
    18181 cagatctatc gcggcatcgt gccgttcgtc agcttgcagc tggtcgggct tctggtggtc
    18241 ataatgttcc cggccctggc gctgtggctg ccgcacgtca tgcttgactg accgtttggc
    18301 cggcggcggc cgcgcgcacc aggcgcacct gctcatcgac cgagatgtcg agcaccgagg
    18361 tcacctcgag cccgcggtcc tcgtagaccg aggtctcggc gccggcgttg ccgagctgcc
    18421 acagccggac cgcctcgtcg cgctcgccga gcagccattc gacctggggc cggaacaggc
    18481 gcagcatcgc gcccacccaa cgattggccg gccacgacgg caccgcatgc gtgatccgga
    18541 accggtcgag catcgcgatc acgtccgcgg ccgcgtacca gctttcgccg gtgacccagc
    18601 ggttggtggt gaacaggcgc aagctgcggc cggccttgtc catcgagatg ccgaccagat
    18661 ggctgagcgc gtcctcgccc agcggccgct cgaccgccgc cggcagcggt gccggctcga
    18721 tcccgtccgg catccccttg gcgcgcacga aggtgtggaa gtggccgtgc tcgccgccgc
    18781 gcaagctcga gggatgggcg tgatagtagt attgggcgtg ggtttggcgg tcgtagacgt
    18841 cgcccttggg gtagtggctc cactcggtga acgccccctg cccgcgcagc acctcgccga
    18901 ccaggttggc gccgccgcgc gcgagcgcgt cctggcattc gagcacctcc tcggcggcgc
    18961 gcgccatgtc ctcggcatgc gccgccggca ccgtccacag cggccgccgg tcgggcccct
    19021 gctcgcggcg ggtcaggctg tccttgagcg cttcgatgtc gaccatccgt gccacccgtc
    19081 tcagtccggc gcgcgcacca ggcgaaaccc gatcaggatg tgcttgagcc cgcccgggcg
    19141 cgcatggcgc gccgccggcc ggcacacgcc gcagcccttg tcgtcccacg agccgccctt
    19201 gaccagatag cgcccggggc cggcctcggt cgaggtccat tcgaacacct ggcctgcggc
    19261 atcgagcacg ccgaacgggc tcgcgccccc cgtgaagctg cctgcggcca tggtcgcgaa
    19321 cggcccccgg tcgtggctgt tgaggcgcgc cggatcgaac gcctcgcccc acgggaagcg
    19381 gcggccgtcg gtgccgcgtg cggccttctc ccattcgagc tcgctcggca gccgccacgt
    19441 caggccggtc gccttgctca gccagccggc ataggccagc gcatcggcgt ggctgaccag
    19501 caccaccggg tggtcggcct tgcccgccgg cggcacgccc tccagccact gatagccgag
    19561 ggtgcgctca taggggtggg ccaggcgata gccgtcccag gtctcgcggt cgaccgcggg
    19621 cgcgggatgg ccggtggcgg cgacgaacgc cgcgtaggcg cggttggtga tcggggtacg
    19681 ggtgatggcg tagcccgcca gctcgaccgg cccgcgcgcg cgttcgcggt cgtaccagcc
    19741 ctgcctgcgg gtcgcgtcgt ggccgtaggc cgccgcgtcg agccggtagg ccgcctcgcg
    19801 ctcggccggg tcagagccgg cgatgaacgg gccgggcgga atccacacca cctcgggcgc
    19861 ttctgccggc tcggcccgcg cgcgccccag cccggccgcg gcggcgagcg cgaccatgac
    19921 caccgccggc ctgcaccacg acatcgccat ggcgcatcct agcatcgccg ccgctgcacc
    19981 cgcaggcgag cctaacgcaa acgtgaaccg gaatgatttg gccgcgtcgg cgcccggcgg
    20041 ctgaatcgcc ctggtttggt gttttcagca agcgcagtgg aggaggagaa tggacaaagt
    20101 caagtcgacc cgcagccggc tgctggcgac aaccgtgctg ccgatggcga tcgtcgccgc
    20161 gatcggcgcg gcctcgccgg cctatgccgc gtgccaggcg tgcaagccgt gcgcggcggc
    20221 ggcgtgcagc gcctgcagcc cgtgcaaggc cagcgcctgc gccgcctgca acccgtgcaa
    20281 cccgtgcgcg gcggcggggt gcaaggcgtg caacccgtgc aatccctgca agccggactg
    20341 acggcgcgct gaggcgacgc cgccccgccg cccgtggcgg ggcggcggac gcccgcccca
    20401 gcccccctcg cgatggtcag cggtccgggt tgaacccggc cggcgcggca attatgttac
    20461 tgcacacacg cgccggcgcc cggatatatg gccgggcgca gcacccgttt gaggcatgat
    20521 gccaaccgag cgaaagggtt tgttcctatg atccagcgca ttctggcgtt cgccgccgcc
    20581 gccggcatcg ccattgccgc ggccgccccg gccggcgccg ccgacctcga ggcgggcgaa
    20641 aaggtattca agaaatgtaa ggcctgtcac accatcgagg ccgacggcaa gaacaagatc
    20701 ggtccgaacc tgcacggcgt cctcggccgc gaggcgggca cgatggaagg gttcaagtat
    20761 tccaaggcga tggcagaggc cgggatcacc tgggacgagg accggctcag gcagtatctc
    20821 gccgacccca aggcgtacat cgagggcaac aagatggcct tccccggcct caaggacgag
    20881 gccgaccggg agaacgtgat cgcctacatc aagcaggaga gcggcgagta agccgagcgc
    20941 ggcgcgcgat cgcgaccggc cgggcagttg cccggccttc gcttttcggg ccggcgttga
    21001 agccgcggcc ggcgcgtggc acgctgccgc gggcgatcgg cggaggcgac catggacctg
    21061 accggcgagt accgcatccc ggcgccgcgc gctgaagtct ggcaggcgct caacgacgcc
    21121 gaggtgctgc gcgcctgcat ccccggcgcc gaggccctcg acaagctctc cgacaccgag
    21181 ttccaggcga ccgtcaaggc caaggtcggg ccggtcgcgg cgcgcttctc gggcaaggtc
    21241 acgctcagcg atctcgtccc gcccgagcat taccggatca gcggcgaggg caccggcggc
    21301 gccgccggct tcgccaaggg cggcgccgag gtgacgctcg ccgaggcgga cgacggcggc
    21361 accgtgctca gctaccgcgc cgacgtccag gtcggcggca agctcgccca gatcggctcg
    21421 cggctggtcg ccggcaccgc gcgcaagatg gccgacgact tcttcggccg cttcgccgag
    21481 cgcctcggcg gcgcccgcgc tgaggccccg gccgaagccg cggccgtggc cccgccgacc
    21541 gaacgccgcg ccttgccgcc cgcaatctgg atcgccgcat tgatcctgct ggtcgtgatc
    21601 ctgctcgtca tcgtcggcgc gtagacgcgc ccggtagcgt ttcggtttga gtgctggcgc
    21661 tgacgggggc cgcgccaccc cggcttcgct cgacggaacg ccatagttcc catgcgaggg
    21721 ccctggaacc ttttggttcc aaggacttaa taaacacgac gagtctgacc atgtggtggt
    21781 cgccgtcgac aacagttggc atgcgcccaa ccgaccgacg atgtatcatg ccgaatcagt
    21841 ggtgaccccg accgctaatc gaggaggaga gcgatggccg cccgcaagcc gttttttggc
    21901 cc
//
LOCUS       DASWHU010000224         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2703267, whole genome shotgun sequence.
ACCESSION   DASWHU010000224 DASWHU010000000
VERSION     DASWHU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703267"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VGB88_08390"
     CDS             <1..826
                     /locus_tag="VGB88_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX9647502.1"
                     /translation="EGGRFEWQTCRAEAPIRRLRGIATEEYAFVRGHTRRVPKVTMPT
                     PSAFHFFRLGDCADAAAYPDLDHFWDDLVAVYRAELAALGDAGCGYVQLDEVPLAMLC
                     DAGVREQVRAQGADPDRLVETYVGMIARVLAGRPPGMRVALHLCRGNFRGRWMAEGGY
                     EPVAEALFNRVDVDAFLLEFDTPRAGGFAPLRLLPAGKRAVLGLVSTKTPRLEATDAL
                     RRRIDQAARLVPLDRLGLSPQCGFASVAGGNPLDEAAQRAKLARVVEVAQAVWGTA"
     gene            complement(995..>1503)
                     /locus_tag="VGB88_08395"
     CDS             complement(995..>1503)
                     /locus_tag="VGB88_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX9647503.1"
                     /translation="GLYGFGAAAHIIAQVARHQRRTVFAFTRPGDAAAQGLARDLGAA
                     WVGGSDQAAPEALDAAIIFAPVGALVPTALAAVRPGGRVVCAGIHMSDIPSFPYELLW
                     HERELVSVANLTRRDGEEFLALAPKVPVKTTIQPYRLADANRALEDLRDGRVQGAAVL
                     VMDGAGTG"
BASE COUNT          192 a          542 c          561 g          208 t
ORIGIN      
        1 cgagggcggc cggttcgagt ggcagacctg ccgcgccgag gcaccgatcc ggcggctgcg
       61 cggcatcgcc accgaggagt acgcgttcgt ccgcggccac acccgccggg tgcccaaggt
      121 gaccatgccg accccgagcg cattccactt cttccgcctc ggcgactgcg cggacgcggc
      181 ggcctatcct gatctcgacc acttctggga cgacctggtc gcggtctacc gggccgagct
      241 cgccgcgctc ggcgatgcgg gctgcggcta cgtccagctc gacgaggtgc cgctcgccat
      301 gctgtgcgac gccggcgtgc gcgagcaggt gcgtgcccaa ggcgccgacc ccgaccggct
      361 ggtcgagacc tatgtcggga tgatcgcccg cgtgcttgcc ggccgcccgc ccggcatgcg
      421 cgtcgcgctc catctgtgcc ggggcaactt ccgcggccgc tggatggccg aggggggtta
      481 cgagccggtg gccgaggcgc tgttcaaccg ggtcgacgtc gatgccttcc tgctcgagtt
      541 cgacaccccg cgcgccggcg gcttcgcgcc cttgcgcctg ctgcccgcgg gcaagcgggc
      601 ggtgctcggc ctggtgtcga ccaagacgcc gcggctggaa gccaccgacg cgctccgccg
      661 gcgcatcgac caggccgcgc gcctggtgcc gctcgatagg ctggggttga gcccgcaatg
      721 cggcttcgcc tcggtcgcgg gcggcaaccc gctggacgag gccgcccagc gcgccaagct
      781 cgcgcgcgtg gtcgaggtcg cgcaggcggt gtgggggacg gcgtagggcc gcccatcgtc
      841 caagccgagt accaaacctc atacttcgag acgcccgctt cgcgggctcc tcagcatgag
      901 gtttggaatg gtgggagcct catcctgagg agggccgcag gcccgtctcg aaggacgagg
      961 cgggcaaccg caacccgtga tttcatccgt tcgctcagcc cgtgccggcg ccgtccatga
     1021 ccaggaccgc cgcgccttgg acccggccgt cgcgcaggtc ttcgagggcg cggttggcgt
     1081 cggcaagccg gtagggctgg atcgtcgtct tgaccggcac cttgggcgcg agcgcgagga
     1141 actcctcgcc gtcgcggcgg gtcaggttgg cgaccgagac cagctcgcgt tcgtgccaca
     1201 gaagctcgta cgggaagctc gggatgtcgc tcatgtggat gccggcgcac accacgcgcc
     1261 cgcccgggcg caccgcggcg agcgcggtgg gcaccagcgc gccgaccggg gcgaagatga
     1321 tcgcggcgtc gagcgcctcg ggcgccgcct ggtcggagcc gccgacccac gctgcgccga
     1381 ggtcgcgcgc gaggccctgg gccgcggcat cgcccgggcg ggtgaaggcg aacaccgtgc
     1441 gccgctggtg gcgggccacc tgggcgatga tgtgcgccgc ggcgccgaag ccgtagaggc
     1501 cca
//
LOCUS       DASWHU010000225         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3422859, whole genome shotgun sequence.
ACCESSION   DASWHU010000225 DASWHU010000000
VERSION     DASWHU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3422859"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..326)
                     /locus_tag="VGB88_08400"
     tRNA            complement(252..326)
                     /locus_tag="VGB88_08400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Val,seq:gac)
     gene            470..1372
                     /locus_tag="VGB88_08405"
     CDS             470..1372
                     /locus_tag="VGB88_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX9647504.1"
                     /translation="MSSPENAAADPPFEIIASDPPATAVVLASPHSGRRYGRAFLSQT
                     RLDEAALRRSEDAHVDALLTHAPARGAALVKALFPRTYVDVNREPLELDPDMFAESLP
                     GCVHATSPRVRSGLGTIARLGANGQPIYRGALSLAEARARLRACYFPYHRALRALVRR
                     AQARFGYAILLDCHSMPSQAVVRPSGLAVRGRGAPRGVDFVLGDCHGGACHAAVADTA
                     QATLERLGHTVIRNAPYSGGFTTRHYGRPASGVHALQIEVNRALYLDEDRLEPTAGLA
                     PLARDLAQLVEALAAALPFARAAE"
     gene            1467..>1778
                     /locus_tag="VGB88_08410"
     CDS             1467..>1778
                     /locus_tag="VGB88_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647505.1"
                     /translation="MGAPALADDAGPWRLAEAAGLPDWLSVTGTHRIRYETLDGQFRA
                     GGKGGDQVLALRTTVLGELRFDGVRIGAELEDSRIELADKGTPLSTSLVNALELLQGY
                     VA"
BASE COUNT          219 a          684 c          621 g          254 t
ORIGIN      
        1 ccctcccacc aggcgtgaaa gcggccgagc atcggcgcca ggcgtgcgcg cgccgggccg
       61 ttgacgatct gcgcggcgaa agcgccggcg gcgccgatcc cggatttcac caagatgcgc
      121 acgctgtgtt ccttcagccc tggccgagcc gtgccttctg gccggcggcc ggtgggtcgc
      181 tccggggact cgatataggg cgatgggcgg cgaagtgtgc cccccagggc ggtccggcgg
      241 gcccgcggcg atggtgggcg cggctgggat tgaaccagcg acccccacga tgtcaacgtg
      301 gtgctctccc gctgagctac gcgcccgccg cccggcacta tagccatgcg ccgaggcttg
      361 gcaagattgg gccgcggccc gcgccggtgg ccgccaagcc gctggcaagc gccgccgcaa
      421 cctcctagac tgccgccccg cctcacgcgc ccagacagga ccttcgcccg tgtcgagccc
      481 cgagaacgcc gcagcggacc cccccttcga gatcatcgct tccgacccgc cggcaaccgc
      541 cgtggtgctt gcctcgccgc atagcgggcg gcgctacggc cgggccttcc tcagccagac
      601 gcggctcgat gaggcggcgc tcaggcgctc ggaggacgcc catgtcgatg cgctcctcac
      661 gcacgcgccg gcgcgcggcg cggcgctggt caaggcgctg ttcccgcgca cctatgtcga
      721 cgtcaaccgc gagccgctcg agctcgaccc cgacatgttc gccgagtccc tgcccggctg
      781 cgtgcacgcg acctcgccca gggtgcgctc gggcctcggc accatcgcgc gcctcggcgc
      841 caacggccag ccgatctatc gcggcgcgct cagcctcgcc gaggcgcggg cgcggctgcg
      901 cgcctgctac ttcccctacc atcgggcctt gcgcgcgctg gtgcgccggg cccaggcgcg
      961 gttcggctac gccatcctgc tcgactgcca ctcgatgccc tcgcaggcgg tggtgcgtcc
     1021 ctcgggcctg gcggtgcgcg ggcgcggggc gccgcggggc gtcgacttcg tgcttggcga
     1081 ctgccacggc ggcgcctgcc acgcggcggt cgccgacacc gcccaggcga cgctggagcg
     1141 gctcggccat accgtgatcc gcaacgcgcc ctattcgggc ggcttcacca cccgccatta
     1201 cggccggccc gcatccggcg tgcacgcgct gcagatcgag gtcaaccggg cgctctatct
     1261 cgacgaggac cggctggagc cgacggcggg gctggcgccg ctcgcccgcg acctcgccca
     1321 attggtcgag gcgctggccg ccgccctccc cttcgcccgc gccgccgagt aggtcgactg
     1381 cgggcggcta atccggctgc gtgcgcacga tcggtatccg tccaggccac ctgacatatt
     1441 tgttggcagg ctcattgttc gcgtcggtgg gcgcgccggc actcgccgac gacgccggcc
     1501 cgtggcggct cgccgaggcg gccggcctgc ccgactggct atccgttacc ggcacgcacc
     1561 gcatacgcta cgagacactc gatggccagt tccgcgccgg cggcaagggc ggcgaccagg
     1621 tgctggcgct gcgcacgacc gtgctcggcg agctgcgctt cgacggcgtg cgcatcggcg
     1681 cggagctcga ggactcgcgt atcgagcttg cggataaggg caccccgctc agcaccagcc
     1741 tggtgaatgc gcttgagctg cttcagggct acgtcgcc
//
LOCUS       DASWHU010000226         8501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2350097, whole genome shotgun sequence.
ACCESSION   DASWHU010000226 DASWHU010000000
VERSION     DASWHU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350097"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..2068
                     /gene="htpG"
                     /locus_tag="VGB88_08415"
     CDS             161..2068
                     /gene="htpG"
                     /locus_tag="VGB88_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX9647506.1"
                     /translation="MNTETAAGERTASPETRAFEAEVGKLLDLMINSLYSHKEIFLRE
                     LISNASDACDRLRYAAITQPELLGDDPALEVRISLDRKARTITVADNGIGMNHDDLVD
                     NLGTIARSGTARFLDRMTGDATKDVSLIGRFGVGFYSAFMVADRVTVTTAKAGESEGW
                     RWTSEGAGGYVVAPLAGRARGTAVELHLRRGEAEFLDAGRLKGIVKTYSDHIALPIRV
                     VGEDGADEVVNQASALWMRPRKDITAQQYAEFYRHVAHAFDEPWLVIHNRAEGKIEYT
                     NLLFVPKTAPFDLFHPDRRHGVKLYVKRVFITDDCEGLVPRWLRFLQGVVDSEDLPLN
                     ISREMLQHNPLLARIRRALVKRVLGELERKAGHAPEEYESFWTAFGAVLKEGLYEDED
                     SRERLLALVRARSTGGDGLVGLADYVARMKDGQGDIFTLTGEDIETVRGSPQLEGFRA
                     RGLEVLLLTDPIDEFWIGAVGEYQGKRFRSVTRGDIDLSKFKADGPAADAAKAAEPVA
                     EGVGELIAAIKQALGARVRDVRVSDRLTDSAVCLVADEGALDMRLERLLRQHRQIDAV
                     ASKVLEINPRNALIKALAGRAKDMPSDAALAEAAELLLDQARIIEGEPLPDPAGFARR
                     MTAALAKGYGA"
     gene            complement(2079..2705)
                     /locus_tag="VGB88_08420"
     CDS             complement(2079..2705)
                     /locus_tag="VGB88_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX9647507.1"
                     /translation="MGTKRVTVRAAGRPALAAAAFAGALAAAAAAAGAVPVLGAPGAW
                     GGNGLYVEAQITLDQMPPQMALAFARGIQEELIAHGYAPGPVDGVVGRRTRQAISAYQ
                     RDAGLPVTGLASGELLDHLKFVTPKVYARKAPASAAAGASKALVTDVQRRLYERGYYL
                     DPIDGLAGPKTRGAVRRFQADANLPQTGVIDERLKSELELAGAALRAR"
     gene            2918..4006
                     /locus_tag="VGB88_08425"
     CDS             2918..4006
                     /locus_tag="VGB88_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX9647508.1"
                     /translation="MLGRLAGAVSAFFAWWGGELAGLVPGFVRRGLAGGRDEAIFAFD
                     DGVLVVSRRGDGGERRLGTVPLAGKSVRALAHHARRLLGRLDPDRVPVMLRVPAGKSL
                     VKSIELPLAAEENLAEVVGFEMDRRTPFKVDDVYFDQRVIARDAKAQRITVELAVAPK
                     HIVAEALATAARWRLWPDRVEVPGDDRPGARRFDLMPRRELGGRRRGRAVRALLTATA
                     CALVAAAVLVPLEHKRRIADHLQDQLSAARAEAEAADALRRQIDSRLAGGRGVVEQRI
                     AIPLAIEVVDEVTRLMPDDTWAFQLQLHGPEVQVFGYAAAASQLIGKFEDSALLSDAR
                     FLSPITRDPRVGLERFHLSAQLAREAGK"
     gene            4003..4599
                     /gene="gspM"
                     /locus_tag="VGB88_08430"
     CDS             4003..4599
                     /gene="gspM"
                     /locus_tag="VGB88_08430"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX9647509.1"
                     /translation="MTGPRGVRGKLVALALVAAAVLAAYALAIEPVYLGYVRYGETIA
                     GSERLLADYRRLGAARPGLEARLGELDRREGRRGDYLQAASDTLAAAALQGELRQLFE
                     RTGAVQRSVQALPATAVDGLVKIAVRAQFSAETEALSAILHELESGRPFLFVDDLDIR
                     RQARRRRARDAETDEDTAAGTLDVRLDLYGYMRPEGGA"
     gene            4613..5257
                     /locus_tag="VGB88_08435"
     CDS             4613..5257
                     /locus_tag="VGB88_08435"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX9647510.1"
                     /translation="MMRGPKSGTASNLLLLLVCLLLATTVWLEIEAAPQPGQPLPPAP
                     GTALEPPPLPDELAYGPPALEDFREVAERPLFAPSRRPPADAPPPVAVAAPAAPELGF
                     ELIGVVISPAETLALVRQPGAPDLVRAGIGQTVAGWTVERIFPNRVLFRAGDRVEELA
                     LRDDVAPPPESASERRERRAEERRRLREERLQDQRLEAERLEAEERAESEQNQR"
     gene            5420..7753
                     /gene="gspD"
                     /locus_tag="VGB88_08440"
     CDS             5420..7753
                     /gene="gspD"
                     /locus_tag="VGB88_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX9647511.1"
                     /translation="MGGSYRRGRRAAAWVAVAIALAGCAELAELRRQDTARVAEVAAA
                     PVGARVAGRDIARTAAEAEAAEAAASDAAVEPGRPETQSVPGTGDFVGAPASVLGAGP
                     GGQAGDITLNFVNADLREVVRTVLGDMLGENYVLDPRVQGTITVQTSRPLARGALLPM
                     LEDVLQLNGFALVRKADSYVVMPLQEAINTAGVSGAFLPGGGVTRGFAIRVVPLAHIS
                     AQEMAGILEPLAPAGGVLRVDQARNLLIIAGTQQELVALDELVRMFDVDWLKGMSFAL
                     VPLDLADAKTVTGELEAVFGSATDGPLAGLLRFVAVERINAVLVISAKPTYLEQAQAW
                     IARLDQGEGESDVRRLFVYYVKNGRAEDLATVLQTAFEPAAAETPRARLAPGAERIEV
                     GEGIRATGADTARAIRELREQRRMARAEAEAADGGEPGAVAPLAAEAAPIEAPPGEAG
                     AVARAAASGGPGISVVGGADIKIIADAANNALVVLASAREYRMIESALKKLDIVPLQV
                     LIEATIAEVTLRDELRYGVQWFLASGNSAISLAATETGEIAQAFPGFSYLLTAGANAR
                     VVLNALDSLTDVNVISSPQLMVLDNQTAELQVGEEVPVATQQQQASTSGDAPLVNTIQ
                     FRDTGVILRVTPRVNPGGLVIMEIEQEVSSVVPSAEPTITPTIQQRRVLSTIAVQSGE
                     TVALGGLIQGERSKTKSGLPFLKDIPLLGELFSATSDTDNRTELLVVITPRVVGNPEE
                     ARAVTQELRNKMRAVGERLGGFGQPAPAPEDEAAPSN"
     gene            7816..>8501
                     /gene="rfbA"
                     /locus_tag="VGB88_08445"
     CDS             7816..>8501
                     /gene="rfbA"
                     /locus_tag="VGB88_08445"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX9647512.1"
                     /translation="MKGIVLAGGAGTRLYPLTHAVSKQLLPVYDKPMVYYPLSTLMLA
                     GIRDILIITAPNEEERFRMVLGDGSQWGIDLSYAVQARPEGIAQAFILGRGFVGDSPV
                     ALVLGDNIFHGHGLTELLVDAARLTTGARVFAYWVDTPSAYGVVSFDADGKAVAIEEK
                     PRRPKSHWAVAGLYFYDPEIVDIAARLTPSARGELEISDVNRIYLEAGTLSVVKLGRG
                     FAWLDTGTHDT"
BASE COUNT         1176 a         3023 c         3172 g         1130 t
ORIGIN      
        1 aggctggtcg acgacgccgc caaggcgctc agcgagggcg ccggggcgct ggccgcggcc
       61 ttcgccgact cttgactcgt cgagtccggg atcacacatc gtcaccgcgg acgatcggca
      121 ctccgcgcgt ggggcagtca gggaggcgga ccggcgggcg atgaacacgg aaaccgccgc
      181 gggcgagcgc acggcaagtc ccgagacccg ggccttcgag gccgaggtcg gcaagctgct
      241 cgacctgatg atcaactcgc tgtactcgca caaggagatc ttcctgcgcg agctgatctc
      301 caacgcctcc gacgcctgcg accggctgcg ctatgcggcg atcacccagc ccgagctgct
      361 gggcgacgac ccggcgctcg aagtgcgaat ctccttggac cgcaaggcgc gcacgatcac
      421 ggtcgccgac aacggcatcg gcatgaacca cgacgacctg gtcgacaacc tgggcaccat
      481 cgcgcgctcg ggcaccgcgc gcttcctcga ccgaatgacc ggcgacgcca ccaaggacgt
      541 gagcctgatc ggccggttcg gggtcggctt ctactccgcc ttcatggtcg ccgaccgggt
      601 cacggtgacc accgccaagg cgggcgagag cgaaggctgg cggtggacct cggagggcgc
      661 gggcggctat gtcgtcgcgc cgctggcagg gcgcgcgcgc ggcaccgcgg tcgagctgca
      721 tttgcgccgg ggcgaggccg agttcctcga tgccggccgc ctcaagggca tcgtcaagac
      781 ctactccgac cacatcgcat tgccgatccg cgtggtcggc gaggacggcg ccgacgaggt
      841 ggtcaaccag gcctcggcgc tgtggatgcg gccgcgcaag gacatcaccg cccagcaata
      901 cgccgagttc taccgccacg tcgcccatgc cttcgacgag ccgtggctgg tgatccacaa
      961 ccgcgccgaa ggcaagatcg agtacaccaa cctgctgttc gtgcccaaga ccgcgccgtt
     1021 cgacctgttc caccccgacc gccgccacgg ggtcaagctg tacgtcaagc gggtgttcat
     1081 caccgacgac tgcgagggcc tggtgccgcg ctggctgcgc ttcctgcagg gcgtggtcga
     1141 cagcgaggac ctgccgctca acatcagccg cgagatgctt cagcacaacc cgctgctggc
     1201 gcgcatccgc cgggcgctgg tcaagcgcgt gctcggcgag ctcgagcgca aggccgggca
     1261 cgcgcccgag gagtacgaga gtttctggac cgcgttcggc gcggtgctca aggaaggcct
     1321 gtacgaggac gaggactcgc gcgagcgcct gctcgccctg gtgcgcgcgc gctcgaccgg
     1381 cggcgacggc ctggtcggcc tcgccgacta cgtcgcgcgc atgaaagacg gccagggcga
     1441 catcttcacc ctcaccggcg aggacatcga gacggtgcgg ggcagcccgc agctcgaggg
     1501 cttccgggcg cgcggcctcg aggtgctgct gctcactgac ccgatcgacg agttctggat
     1561 cggcgcagtc ggcgagtatc agggcaagcg cttccgctcg gtcacccgcg gcgacatcga
     1621 cctgtcgaag ttcaaggccg atgggcccgc ggccgatgcc gccaaggcgg cggagcccgt
     1681 ggccgagggg gtgggcgagc tgatcgcggc gatcaagcag gcgctgggcg cgcgggtgcg
     1741 cgacgtgcgc gtctcggatc ggctgaccga cagcgcggtg tgcctggtcg ccgacgaggg
     1801 cgcgctcgac atgcgcctcg agcggctgct gcgccagcac cgccagatcg acgcggtggc
     1861 gagcaaggtg ctcgagatca acccgcgcaa cgccttgatc aaggcgctcg ccgggcgcgc
     1921 caaggacatg ccctccgacg ccgcgctggc tgaggcggcc gagctcttgc tcgatcaggc
     1981 gcggatcatc gagggcgagc cgctgcccga cccggcgggc ttcgcgcgcc gcatgaccgc
     2041 ggcgctggcc aagggctacg gcgcctgagc ggcgcggcct agcgggcgcg cagcgccgcc
     2101 ccggccagct cgagctcgga cttgaggcgc tcgtcgatca ccccggtctg cggcaggttg
     2161 gcgtcggcct ggaagcggcg caccgcgccg cgggttttcg gcccggccag gccgtcgatc
     2221 gggtcgaggt agtagccgcg ctcgtagagc cggcgctgga cgtcggtgac cagggccttc
     2281 gatgcgccgg cggcggcgct cgccggcgcc ttgcgggcgt acaccttcgg ggtcacgaac
     2341 ttgagatggt cgagcagctc gccgctggcg aggcccgtca ccggcagtcc ggcgtcgcgc
     2401 tgataggcgc tgatcgcctg gcgggtgcgc cggccgacca cgccgtcgac cgggcccggg
     2461 gcatagccgt gggcgatcag ctcctcctgg atgccgcgtg cgaacgccag cgccatctgc
     2521 ggcggcatct ggtcgagggt gatctgggcc tcgacataga ggccattgcc gccccaggcg
     2581 ccgggcgcac cgaggaccgg caccgcgccc gccgccgcgg ctgccgcggc cagcgccccg
     2641 gcgaacgccg ccgcggccag cgccggccgg cccgccgctc gcaccgtcac gcgcttggtt
     2701 cccatcttgc cctccgtcag ctcggttgcg gacacgcctc ccccgtctag atagcggcgc
     2761 agcgcgcgcc ccgcaatcgc cccgccacgc gcccgcccgg ggggcggcac cggcgcgccc
     2821 tggcgcaggc gcggcaactg cttatatttg ctggcggagc caagacgcgg ccgcgcgccg
     2881 gccacgcgcg gtcggccaag gcaacgcgag gatggggatg ctcggtcgat tggcgggcgc
     2941 agtcagcgct ttcttcgcct ggtggggcgg cgagctggcc gggctggtgc ccggcttcgt
     3001 gcgccgcggc ctggccggcg gccgcgacga ggcgatcttc gccttcgacg acggcgtgct
     3061 ggtggtctcg cggcgcggcg acggcggcga gcggcggctc ggcacggtgc cgctggccgg
     3121 caagtcggtg cgcgccctcg cccaccacgc ccgccgcctg ctcggccggc tcgacccgga
     3181 ccgggtgccg gtgatgctgc gggtgccggc gggcaagtcg ctggtcaagt cgatcgagct
     3241 gccgctcgcg gccgaggaga acctggccga ggtggtcggc ttcgagatgg atcggcgcac
     3301 cccgttcaag gtcgacgacg tgtatttcga ccagcgcgtg attgcgcgcg acgccaaagc
     3361 gcagcggatc acggtcgagc tcgcggtggc gcccaagcat atcgtcgccg aggcgctggc
     3421 caccgcggcc cgctggcggc tgtggccgga ccgggtggag gtgccgggcg acgaccgccc
     3481 cggcgcccgc cggttcgacc tgatgccgcg gcgcgagctc ggtggcaggc gccggggccg
     3541 ggcggtgcgc gcgctgctca ccgccactgc ctgcgcgctg gtcgcggcgg cggtgctggt
     3601 gccgctcgag cacaagcggc ggatcgccga ccatcttcag gaccagctca gcgccgcccg
     3661 cgccgaggcc gaagcggcgg acgcgctcag gcgccagatc gacagccggc tggcgggcgg
     3721 gcgcggcgtg gtcgagcagc ggatcgcgat cccgctcgcg atcgaggtgg tcgacgaggt
     3781 cacccgcctg atgcccgacg atacctgggc gttccagctt cagctccacg gcccggaggt
     3841 gcaggtgttc ggctatgccg cggcggcatc gcagctgatc ggcaagttcg aggactcggc
     3901 gctgctgtcg gacgcccgct tcctgtcgcc gatcacgcgc gatccgcgag tcgggctgga
     3961 gcgcttccac ctgtcggcgc agctcgcgcg cgaggcgggc aagtgaccgg cccgcgcggc
     4021 gtgcgcggca agctggtcgc gctggccttg gtggcggcgg cggtgctggc ggcctacgcg
     4081 ctggcgatcg agccggtcta tctgggctac gtccgctatg gcgagaccat cgccgggtcc
     4141 gagcgcctgc tcgccgatta ccgccggctg ggcgcggcgc gccccgggct ggaggcgcgg
     4201 ctgggcgagc tcgaccggcg cgagggccgg cgtggcgact acttgcaggc ggcgtccgac
     4261 acgctcgccg ccgcggcgct ccaaggcgag ctgcgccagc tgttcgagcg caccggcgcg
     4321 gtccagcgca gcgtccaggc gctgcccgcg accgcggtcg acggcctggt aaagatcgcg
     4381 gtccgcgccc agttcagcgc cgagaccgag gcgctctcgg cgatcctcca cgagctcgag
     4441 tcggggcggc cgttcctgtt cgtcgacgat ctcgacatcc gccgccaggc ccgccggcgg
     4501 cgggcgcgcg atgccgagac ggacgaagac accgccgccg gcacgctcga cgtgcgcctc
     4561 gacctgtacg gctacatgcg ccccgagggc ggcgcgtgag ggggatggcg cgatgatgcg
     4621 cggcccgaag agcggaaccg cctcgaactt gctgctgctg ctggtgtgcc tgctgctcgc
     4681 cacgacggtg tggctcgaga tcgaggccgc gccgcagccc ggccaaccgc tgccgccggc
     4741 gccgggtacc gcgctcgagc cgccgccgct gcccgacgag cttgcctatg ggccgccggc
     4801 gctggaagac ttccgcgagg tggccgagcg gccgctgttc gcgccctcgc ggcggccgcc
     4861 ggcggacgcg ccgccgccgg tcgcggtggc ggcaccggcg gcgccggagc tcggcttcga
     4921 gctcatcggc gtggtgatct cgccggccga gaccctggcc ctggtgcgcc agcccggggc
     4981 gcccgacctg gtacgcgccg gcatcggcca gacggtggcg ggctggacgg tcgagcgcat
     5041 ctttcccaac cgggtgctgt tccgcgccgg cgacagggtc gaggagctgg cgctgcgcga
     5101 cgacgtggcg ccgccgccgg agagcgcgag cgagcgccgc gagcgacgcg ccgaggagcg
     5161 ccgccggctg cgcgaggagc gcttgcagga ccagcgactc gaggccgagc gactggaggc
     5221 cgaggagcgc gccgagtccg agcagaacca gcgctgagcg ccggcggccg ccctgcaagt
     5281 cggcggccgt agatgctttt tagcaacagt gtcacatgtt tagcgcggga ttaaattgac
     5341 atgcgcgtcc agccgtggcg gagaatccag gctgggcagt atgccggagg cggggaacat
     5401 aggctgccaa cacgcagcgg tgggcgggtc gtatcggcgt gggcgcaggg cggcggcgtg
     5461 ggtcgcggtc gcgatcgcgc tggccggctg cgccgagctc gccgagctgc gacgccagga
     5521 caccgcgcgg gtggccgagg ttgcggcggc gccggtcggc gcgcgcgtcg ccggccgcga
     5581 tatcgcccgg accgcggccg aggccgaggc ggccgaggcg gcggcctccg acgccgcggt
     5641 cgagccgggc cgccctgaga cccagtcggt gcccggcacc ggcgacttcg tcggcgcgcc
     5701 ggcgagcgtg ctcggcgccg gtcccggcgg gcaggcgggc gacatcacgc tcaacttcgt
     5761 caacgccgac ctgcgcgagg tggtgcgcac cgtgctcggc gacatgctgg gcgagaacta
     5821 cgtgctcgat ccccgcgtcc agggcacgat cacggtccag accagccggc cgctggcccg
     5881 cggcgcgctg ctgccgatgc tcgaggacgt gctccagctc aacggcttcg cgctggtgcg
     5941 caaggcggac agctacgtgg tgatgccgtt gcaggaggcg atcaacacgg ccggcgtctc
     6001 gggcgcgttc ctgcccggcg gcggggtcac ccgcggcttc gccatccggg tggtgccgct
     6061 tgcccatatc agcgcccagg agatggccgg catcctcgag ccgctggcgc ctgcgggcgg
     6121 cgtgctgcga gtcgaccagg cgcgcaacct gctgatcatc gccggcaccc agcaggagct
     6181 ggtggcgctg gacgagctgg tccgcatgtt cgacgtcgac tggctcaagg gcatgtcgtt
     6241 tgccctggtg ccgctcgatc tggccgacgc caagaccgtg accggggagc tcgaagcggt
     6301 gttcggcagc gccaccgacg gcccgctggc gggcctgctg cgcttcgtcg cggtcgagcg
     6361 catcaacgcc gtgctggtga tcagcgccaa gccgacctat ctcgagcagg cgcaggcgtg
     6421 gatcgcgcgc ctcgaccagg gcgagggcga gagcgacgtc cgccggctgt tcgtctatta
     6481 cgtcaagaac ggccgcgccg aggatctcgc caccgtgctg cagaccgcgt tcgagccggc
     6541 ggccgccgag accccgcggg cgcgcctcgc ccccggcgcc gagcgcatcg aggtcggcga
     6601 gggcatccgc gccaccggcg ccgataccgc gcgcgcgatc cgcgagctgc gcgagcaacg
     6661 gcgcatggcg cgcgccgaag ccgaggccgc ggacggcggc gagcccggcg cggtcgcgcc
     6721 gctggccgcc gaggcggcac cgatcgaggc cccgcccggc gaggccggcg ccgtcgcccg
     6781 cgccgccgcg agcggcgggc ccggcatctc ggtggtcggc ggggccgaca tcaagatcat
     6841 cgccgacgcc gccaacaacg cgctcgtggt gctggccagc gcgcgcgagt accggatgat
     6901 cgagagcgcg ctcaagaagc tcgacatcgt gccgctgcag gtgctgatcg aggcgaccat
     6961 cgccgaggtc acgctcaggg acgagctgcg ctacggcgtg cagtggttcc tggccagcgg
     7021 caactccgcg atcagtctcg cggccaccga gaccggcgag atcgcgcagg cgttccccgg
     7081 cttctcgtac ctgctgaccg cgggcgcgaa tgcgcgcgtg gtgctgaacg cgctcgattc
     7141 gctcaccgac gtcaacgtca tctcgtcgcc gcagctgatg gtgctcgaca accagaccgc
     7201 cgagctccag gtcggcgagg aggtgccggt cgcgacccag cagcagcagg cgagcacctc
     7261 gggcgatgcg ccgctggtca ataccatcca gttccgcgac accggcgtca tcctgcgcgt
     7321 caccccgcgc gtgaacccgg gcgggctggt gatcatggag atcgagcagg aggtctcgag
     7381 cgtggtgccg agcgccgagc cgaccatcac cccgaccatc cagcagcgcc gggtgctgag
     7441 cacgatcgcg gtccagtcgg gcgagacggt ggcgctcggc ggcctgatcc agggcgagcg
     7501 cagcaagacc aagagcgggc tgccgttcct caaggacatc ccgctgctgg gcgagctgtt
     7561 cagcgccacc agcgacaccg acaaccgcac cgagctgttg gtggtgatca ccccgcgcgt
     7621 agtcggcaac ccggaggagg cgcgggccgt gacccaggag ctgcgcaaca agatgcgcgc
     7681 ggtgggcgag cgcctgggcg gcttcggcca accggcgccg gcacccgagg acgaggccgc
     7741 gcccagcaac tgaccggccc gccgcgtgcg tcggcagcgg cgcgcagtgg tcggcaattg
     7801 gagaggcagt gcgggatgaa gggaatcgtc cttgccggcg gcgcgggcac gcggctctat
     7861 ccgctgaccc acgcggtgtc caagcagctg ctgccggtct acgacaagcc gatggtctac
     7921 tatccgttgt cgacgctcat gctcgccggc atccgcgaca tcctgatcat caccgcgccg
     7981 aacgaggaag agcggttcag gatggtgctg ggcgacggct cgcaatgggg catcgacttg
     8041 tcctatgccg tgcaggcccg tcccgagggt atcgcccagg cgttcatcct cggccgcggc
     8101 ttcgtcggcg acagcccggt ggcgctggtg ctgggcgaca acatcttcca cggccacgga
     8161 ctgaccgagc tgctggtcga tgccgcgagg ctgaccacgg gcgcccgcgt gttcgcctac
     8221 tgggtcgaca ccccgtcggc ctacggcgtg gtgagcttcg atgccgacgg caaggcggtc
     8281 gcgatcgagg agaagccgcg ccggccgaaa tcccactggg cggtcgccgg gctgtatttc
     8341 tacgaccccg agatcgtcga catcgccgcc cggctcacgc cgtcggcgcg cggcgagctc
     8401 gagatcagcg atgtcaaccg gatttatctc gaggccggca cgctgtcggt ggtcaagctc
     8461 gggcgcggct tcgcctggct cgataccggc acccacgaca c
//
LOCUS       DASWHU010000227         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_107606, whole genome shotgun sequence.
ACCESSION   DASWHU010000227 DASWHU010000000
VERSION     DASWHU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107606"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VGB88_08450"
     CDS             <1..1039
                     /locus_tag="VGB88_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647513.1"
                     /translation="VRERDGFDGYWLAEGAGQGAGLDFGIGALVDDAGGDRYQARRFA
                     QGASRRGGLGVLADDAGGDVYALDLPGDGWGRGRGLAGLPALAFLIDDGESDALMLGG
                     APLLDRAPVALAGPLAGRASGLDSEPAPACPAPGEGAGAYHDGDPVGAWLAQAPLLAR
                     GDDAGAELSARLWASLPASVPALLHEVPTGEPLLAAKLETLLRCYLIAGDPLAAAEAR
                     GWLIEAVKGGTGHAAAALAALAAAPPDAASALSVAAVAAGAPDCATRAGAIDLVAAVA
                     ARDPTAADDVAAYALGAAGDRCWQAAGAALELWRAVRGPEEPLPVAVAELLPAALRSN
                     PAAAGAPEPEE"
     gene            complement(993..>2343)
                     /locus_tag="VGB88_08455"
     CDS             complement(993..>2343)
                     /locus_tag="VGB88_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX9647514.1"
                     /translation="ATYVVLFVMFGAFLERVGTGELFMSFALALTGHTPGGPAKVACL
                     TSGLFGTVSGSAVSNVMTTGTLTIPLMRRIGYRPAFAGAVEAVAATGGQIMPPIMGAA
                     AFVMAEFLGVSFMTIAGYAVVPAVLFYVALFMAVHFEAKRTGMSGVARSELPDLRKVL
                     ASRGHLFVPMVVIIGVLVAGYSAPYAALCGIGVIVPVALLRRETRRTVTPAMIVDALA
                     TGARNTLAVAAACACAGIVIGVISLTGIGLAFTTLVIDAAQDSLWPALVLTMVAGIVL
                     GMGLPTTPAYIMQVALLVPALVHLGVLQPAAHMFVFYFAILSSITPPVAMAVFAANSI
                     SQARLMETGLAAVKLGATGYLVPFLFVFAPALLLIGDAAHVALAVVTAVVGVSCLAGS
                     LHGYFVGPASWLDRALLFATAIALLIPGVPATVVGVSLLALTVTLQVLARRPQPGST"
BASE COUNT          339 a          857 c          881 g          266 t
ORIGIN      
        1 ggtgcgcgag cgcgacggct tcgacggcta ctggctggcg gaaggggcgg ggcagggcgc
       61 cgggctcgac ttcggcatcg gggcattggt cgacgacgcg ggcggcgacc gctaccaggc
      121 gcgccgattc gcgcaagggg caagccgccg cggcgggctc ggcgtgctcg cggacgacgc
      181 gggcggcgac gtctacgccc tggacctgcc cggcgacggc tggggccgcg gccgcgggct
      241 tgcggggctg ccggcgctcg ccttcctcat cgacgacggc gagtcggacg cgctgatgct
      301 cggcggcgcg ccgctgctcg accgcgcacc ggtcgcgctc gccggcccgc ttgccggtcg
      361 cgcgtcgggc ctcgactctg agccggcgcc ggcctgcccg gcgccgggcg agggtgccgg
      421 tgcctatcac gacggcgatc cggtcggggc gtggctcgcc caggcgccgc tgctcgcccg
      481 cggcgatgac gcgggcgcgg agctctccgc gcgcctgtgg gcgagcctgc ctgcaagcgt
      541 gccggccttg ctgcacgagg tgccgaccgg cgagccgctg ctcgcggcca agctcgagac
      601 gttgttgcgc tgctatctga tcgccggcga tccgttagcg gcggcagagg cgcgcggctg
      661 gctgatcgag gcggtcaagg gcggcaccgg ccacgccgcg gcggcgctcg cggcgctcgc
      721 cgccgcgccg cccgatgcgg cgtcggcgct gtcggtcgcg gccgtcgcgg ccggcgcacc
      781 cgactgcgcg acccgcgccg gcgcgatcga tctggtcgcc gcggtggcgg cccgcgatcc
      841 gacggctgcc gatgatgtcg ccgcctatgc cttgggcgcc gccggcgacc gctgctggca
      901 ggcggccggc gcggcgctcg agctgtggcg ggcggtgcgc gggcctgagg agccgctgcc
      961 ggtggcggtg gccgagctgc tgccggccgc tctcaggtcg aacccggctg cggccggcgc
     1021 gccagaacct gaagagtgac ggtcagcgcg agcaggctga cgccgaccac cgtcgccggg
     1081 acgccgggaa tcaaaagcgc gatcgcggtc gcgaacagca gcgcccggtc gagccagctt
     1141 gccgggccga caaaatagcc gtgcaacgaa ccggcgaggc agctcacccc gaccaccgcg
     1201 gtgaccaccg ccagcgcgac atgcgcggca tcgccgatca gcagcagcgc cggcgcgaac
     1261 acgaacagga acggcaccag gtagccggtg gcgccgagct tgaccgccgc cagccccgtc
     1321 tccatcagcc gcgcctgcga gatgctgttg gccgcgaaca ccgccatcgc caccggcggc
     1381 gtgatcgagg acaggatcgc gaagtagaac acgaacatgt gcgccgccgg ctggagcacg
     1441 ccgagatgca ccagcgccgg caccagcagc gccacctgca tgatgtaggc gggcgtggtc
     1501 ggcagtccca tgcccagcac gatgccggcg accatggtca gcaccagcgc cggccacagc
     1561 gagtcctggg cggcgtcgat caccagggtg gtgaaggcga ggccgatgcc ggtcaggctg
     1621 atcacgccga tgacgatgcc ggcgcaggcg caggcggcgg cgacggcgag cgtgttgcgc
     1681 gcgccggtgg ccagcgcatc gacgatcatc gccggcgtga ccgtgcgccg ggtctcgcgc
     1741 ctgagcagcg ccaccggcac gatcacgccg atcccgcaca gcgccgcata gggcgcgctg
     1801 tagcccgcca ccagcacgcc gatgatcacc accatcggca cgaacaggtg gccgcgcgag
     1861 gcgagcacct tgcgcaggtc gggcagctcg ctccgcgcca cgccgctcat gccggtgcgc
     1921 ttggcctcga agtgcaccgc catgaacagg gcgacgtaga acagcaccgc cggcaccacc
     1981 gcgtagccgg cgatggtcat gaagctcacg cccaggaact cggccatcac gaacgccgcg
     2041 gcacccatga tcggcggcat gatctggccg ccggtcgcgg ccaccgcctc gaccgcgccg
     2101 gcgaacgcgg gccggtagcc gatccggcgc atcaagggga tggtcaaggt gccggtggtc
     2161 atcacgttcg agaccgcgct gcccgagacg gtgccgaaca ggccgctggt caggcacgcc
     2221 accttggccg ggccgccggg ggtgtggccg gtcagcgcca gcgcgaacga catgaacagc
     2281 tcgccggtgc cgacccgctc gagaaacgcg ccgaacatca cgaacagcac gacataggtc
     2341 gcc
//
LOCUS       DASWHU010000228         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2704206, whole genome shotgun sequence.
ACCESSION   DASWHU010000228 DASWHU010000000
VERSION     DASWHU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704206"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGB88_08460"
     CDS             complement(<1..758)
                     /locus_tag="VGB88_08460"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976147.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEX9647515.1"
                     /translation="MCGIIGAVGRSQATPLLLDGLRRLEYRGYDSAGIATLVNGHIER
                     RRAEGKLDRLVELVERTPLPGTIGIGHTRWATHGMANEGNAHPHASDRVAVVHNGIIE
                     NFQALRDELQSAGSNFASDTDTEVVVHLLSRALAHGASPQAATEAMLARIEGAFSLAI
                     LFAGHDDLLIAARRGSPLAIGFGDGEMFLGSDAMTLAPLTRRICYLEEGDWAELSRAG
                     AVVHDQTNAVVARPVRETALTGAAIGKGNHRHFM"
     gene            complement(762..2144)
                     /gene="glmU"
                     /locus_tag="VGB88_08465"
     CDS             complement(762..2144)
                     /gene="glmU"
                     /locus_tag="VGB88_08465"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX9647516.1"
                     /translation="MSATGPSGRSRAATGDIAVVVLAAGKGTRMRSALPKVMHPLAGR
                     PMICQVIETAAALAPARIVVVVGAEMDGLGQAIAPHPTVVQEPQLGTGHAVQQARAHL
                     DGFAGEVVVLYGDTPLIRAETLRALVAARRGGAAPAVAVLGFRLADPGAYGRLVMAAD
                     GTLARIVEAADSDQRTPADVLCNSGVMAVDSALLFELLDRLGTDNAKGEYYLTDIVAH
                     ARAAGRAAAVVEAAADELVGVNTRAELAAAEAVVQGRLRARAMADGATLTDPASVWLC
                     WDTALGRDVRIGPNVVFGPGVRIQDDVDILPFCHLEGVVVASGARIGPFARIRPGTEV
                     GAGARVGNFVEAKAARIEAGAKVNHLSYVGDARVGAGANVGAGTITCNYDGVTKGRTD
                     IGAGAFIGSNTALVAPVKVGDGAMVGAGSTITGDVAADALALARAEQRNLPGGATRFR
                     ARRRKSTPAG"
     gene            2216..>2870
                     /gene="gph"
                     /locus_tag="VGB88_08470"
     CDS             2216..>2870
                     /gene="gph"
                     /locus_tag="VGB88_08470"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125083.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX9647517.1"
                     /translation="MPELPGPIVFDLDGTLIDSAADLTEALNRVLADQGLPAVAEASV
                     RHMVGDGAVKLIERGFAAAGRPFDDGLPARLRQAFLDHYGACLIQRTRAQPGAAEALA
                     ALSAAGRRMAVCTNKPAAMSETILAHLGLAGHFEAVLGGDSLAVRKPDPAPLFAAIER
                     SGGAAPRAVMVGDSHTDVATARAAAVPVVVVSFGYTRTPARALGADAVIDHFDELLPA
                     "
BASE COUNT          376 a         1067 c         1055 g          372 t
ORIGIN      
        1 tccatgaaat ggcggtggtt gcccttgccg atggcggcgc cggtgagcgc ggtctcgcgc
       61 accgggcggg cgaccacggc gttggtctgg tcgtggacca ccgcgccggc gcgcgacagc
      121 tcggcccagt cgccctcctc cagatagcag atgcgccggg tcagcggcgc cagcgtcatg
      181 gcgtcggagc cgaggaacat ctcgccgtcg ccgaagccga tggcgagcgg gctgccgcgg
      241 cgagccgcga tcagcaggtc gtcgtggccg gcgaacagga tggcgagcga gaacgcgccc
      301 tcgatgcgcg cgagcatcgc ctcggtcgca gcttgcgggc tggcgccgtg cgcgagcgcg
      361 cgcgacaaca ggtgcaccac cacctcggtg tcggtgtcgc tggcgaagtt cgagcccgcg
      421 gactgaagct cgtcgcgcaa cgcctggaag ttctcgatga tgccgttgtg gaccacggcc
      481 acccggtcgc tggcatgggg atgcgcgttg ccctcgttcg ccatgccgtg ggtcgcccag
      541 cgggtgtggc cgatgccgat ggtgccgggc aagggcgtgc gctcgaccag ctcgaccagg
      601 cggtcgagct ttccttccgc gcggcggcgc tcgatatggc cgttgaccag ggtggcgatc
      661 ccggccgagt cgtagccgcg gtattcgagc cgccggagcc cgtcgagcag gagcggcgtc
      721 gcctgcgacc tgccgaccgc gccgatgatt ccgcacatgc ctcagccggc cggtgtggac
      781 ttgcggcggc gggcgcggaa ccgcgtcgcg ccgccgggca ggttgcgctg ctcggcgcgg
      841 gcgagcgcca gcgcgtccgc agccacgtcg ccggtgatcg tgctgccggc gccgaccatc
      901 gcgccatcgc cgaccttgac cggcgccacc agcgccgtgt tcgagccgat gaacgcgccg
      961 gcgccgatgt cggtgcggcc cttcgtgaca ccgtcatagt tgcaggtgat ggtgccggcg
     1021 ccgacattgg cgccggcgcc gacgcgggca tcgccgacat agctcaggtg attgaccttg
     1081 gcgccggcct cgatgcgcgc cgccttggcc tcgacgaagt tgccgacgcg cgcgccggcg
     1141 ccgacctcgg tgccggggcg gatgcgcgcg aacgggccga tgcgcgcgcc cgacgccacc
     1201 accacgcctt cgaggtggca gaacggcagg atgtcgacgt cgtcctggat gcgcacgccg
     1261 gggccgaaca cgacgttggg gccgatgcgc acgtcgcggc cgagcgcggt gtcccagcac
     1321 agccagacgc tcgccgggtc ggtgagcgtc gcgccgtcgg ccatggcgcg ggcgcgcagc
     1381 cggccctgga ccaccgcctc ggccgcggcg agctcggcgc gggtgttgac cccgaccagt
     1441 tcgtcggccg cggcctcgac caccgccgcc gcccgcccgg ccgcgcgcgc atgggcgacg
     1501 atatcggtca ggtaatactc gcccttggcg ttgtcggtgc cgagacggtc gagcagctcg
     1561 aacaggagcg cgctgtcgac cgccatcacg cctgagttgc acagcacatc cgcgggcgtt
     1621 cgctggtcgg agtcggcggc ctcgacgatg cgcgcgagcg tgccgtcagc ggccatgacc
     1681 aggcggccgt aagcgcccgg atcggccagc cggaagccga gcaccgcgac cgcgggcgcc
     1741 gccccgccgc gccgggcagc gaccagcgcg cgcagcgtct cggcgcgaat caggggcgtg
     1801 tcgccgtaca gcacgaccac ctcgccggcg aagccgtcga gatgcgcgcg cgcctgctgg
     1861 accgcgtggc cggtgccgag ctggggctcc tggaccacag tcgggtgcgg cgcgatcgcc
     1921 tggccgagcc cgtccatctc cgcgccgacc accaccacga tccgcgccgg cgcgagcgca
     1981 gccgcggtct cgatcacctg gcagatcatc ggccggccgg cgagcgggtg catcaccttg
     2041 ggcagggccg agcgcatgcg cgtgcccttg ccggccgcga gcacgaccac cgcaatgtca
     2101 cccgttgccg ccctggaacg gcccgagggg cctgtcgccg acatggactt accgtataca
     2161 agcaccaatc taagcgaggc aagcgagccc cgcgccgcca tcatagcact ccgccatgcc
     2221 cgagcttccc ggccccatcg tcttcgacct cgacggcacc ttgatcgaca gcgccgccga
     2281 cctgaccgag gcgctcaacc gcgtgctcgc cgaccagggc ctgccggcgg tggccgaggc
     2341 gtcggtccgc cacatggtcg gcgacggcgc ggtcaagctg atcgagcgcg gcttcgccgc
     2401 cgccggcagg ccgttcgacg acggcctgcc ggcgcgcctg cgccaggcgt tcctcgacca
     2461 ctacggggcg tgcctcatcc agcgcacgcg cgcccagccg ggcgcggccg aggcgctggc
     2521 cgcgctcagc gcggcggggc ggcggatggc ggtgtgcacc aacaagccgg cggcgatgag
     2581 cgagacgatt ctcgcccatc tcggccttgc cggccacttc gaggcggtgc tcggcggcga
     2641 cagcctggcg gtgcgcaagc ccgacccggc gccgctgttc gccgcgatcg agcgcagcgg
     2701 cggcgccgcc ccgcgcgcgg tcatggtcgg cgacagccac accgacgtcg ccaccgcgcg
     2761 cgccgccgcg gtgccggtgg tggtggtgag cttcggctac acccgcaccc cggcgcgcgc
     2821 gctcggcgcg gacgcggtca tcgaccattt cgacgagctg ctgccggcgc
//
LOCUS       DASWHU010000229         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_492955, whole genome shotgun sequence.
ACCESSION   DASWHU010000229 DASWHU010000000
VERSION     DASWHU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492955"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1355)
                     /locus_tag="VGB88_08475"
     CDS             complement(147..1355)
                     /locus_tag="VGB88_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647518.1"
                     /translation="MAWRKAIVTGLAAGAVLAAAASLAADYDVGADDERILIGSTHPY
                     SGPASAYGAIGRAMSAYFDMVNADGGVGGRRVDFVSLDDGYSPPKTVEQVRKLVEHDR
                     VLMLFGNLGTPTNNAVVKYVNAKGVPHLFLTTGSSHWDQPKARPWSMGFWPSYFTEGK
                     VYANYILGNHPRAHIAVLYQNDDYGKDYVAGLKAGLGGRAAEMVVAEAAYEVTDPTVD
                     GLIVQLAGSAAEVLYDISTPKFSAQAIRRAHDLGWRPLHVIGNGSSSIEAVLKPAGLD
                     KSVGVVTAAYLKDPTDARWRHDADVAAWLAWMERYYPDGNTADMFNVYGYASAYVMAE
                     VLRRCGDELTRANVLRQATSLADLEVPMLLPGILVTTGPDDYRPIERLQPMRFDGARW
                     QPIGGLITAD"
     gene            1434..3095
                     /locus_tag="VGB88_08480"
     CDS             1434..3095
                     /locus_tag="VGB88_08480"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX9647519.1"
                     /translation="MPGRASMKVADALVGILQAEGVEQAFCYPFSPVMDAMARAGLKP
                     IVTRQERVAGNMADGVSRSTNGRKTGTVTVQALAGSENAFAGIAQAFTDSTPVLFLPG
                     HPGTRFIGTSPTFDSASNYAGITKHAEKLLLPGELSAQLRRAFTALRSGRPRPVMLEL
                     PADVCAAEIAAPPAYAPVRRMRAAPDAGAVEEAAERLLAAKAPLIWAGQGTLYAEASE
                     ELVRLAELLGAPVMTTLQGKSAMPETHPLSAGVGALVSTAMVQAALREADVLLAVGTS
                     LSRAPFTPAIPDGKDIIHVTVDAGDINKDYGAALGVPADAKLFLAALADEVRRRVGDN
                     ARADQCAEVARKLAETRVAWQAEYEPEFADDSEPINGYRMFRELWNSVDPAATMITHE
                     SGASRDIQCVFYRATTPRGYLGWGQSSQLGFSIGLAMGAKIANPDKLVVNVMGDGAIG
                     MTGMDLETAARNGIAILTVVKHDSIFSGYDRNIPDSVARYQASTQRGDYAQIAGALGC
                     HAERVERVGELRPALARAIGATRDGQPALVDVICAETRKLSRLPA"
     gene            3277..>3794
                     /locus_tag="VGB88_08485"
     CDS             3277..>3794
                     /locus_tag="VGB88_08485"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX9647520.1"
                     /translation="MAEEGKGPTRREGRYDRHSVLIRDGRAIAGELTLDRQGFVLLHR
                     DTAVSDFYDRDEVKSVYYPEVERLVKDATGAAKVIAFDHNLRASDDSIRGAHGVREPV
                     KVVHNDYTPRSGPQRVRDVIGGGEAEALLAHRFAVINVWRPIRGPVKCAPLAVCDAQS
                     IAPGDLVATDLV"
BASE COUNT          571 a         1356 c         1321 g          546 t
ORIGIN      
        1 cagggtgagg taaatgattg attctcaaac ccccctcgtc ctgaggagcg agcggcccgt
       61 cctccgcagc agcggcagcg ccgctgccgc tgcggagggt ggaagcgagc gtctcgaagg
      121 gcgacagccg attcattcgc aaccgctcaa tccgccgtga tcagaccgcc gatcggctgc
      181 cagcgcgcgc cgtcgaagcg catcggctgc aggcgctcga tcgggcggta gtcgtcgggg
      241 ccggtggtca ccaggatgcc cggcagcagc atcggcacct cgagatcggc gagcgaggtc
      301 gcctggcgca gcacgttggc gcgggtcagc tcgtccccgc agcgccgcag cacctcggcc
      361 atgacgtagg cgctggcata gccgtagacg ttgaacatgt ccgcggtgtt gccgtccggg
      421 tagtagcgct ccatccaggc gagccacgcc gcgacgtcgg cgtcgtgccg ccagcgcgca
      481 tcggtcgggt ccttgagata ggcggcggtg accaccccga ccgacttgtc gagccccgcc
      541 ggcttgagca ccgcctcgat cgaggacgag ccgttgccga tgacgtgcag cggccgccag
      601 cccaggtcat gggcgcggcg gatcgcctgg gcggagaact tgggggtcga gatgtcgtac
      661 agcacctccg cggccgagcc cgcaagctgc acgatcagcc cgtcgacggt cggatcggtg
      721 acctcgtagg ccgcctccgc gaccaccatc tcggcggcgc gaccgccgag cccggccttg
      781 agcccggcca cgtagtcctt gccgtagtcg tcgttctggt acagcaccgc gatatgcgcc
      841 cgcggatggt tgccgaggat atagttggca tacaccttgc cttcggtgaa ataggacggc
      901 cagaagccca tcgaccacgg ccgcgccttc ggctggtccc agtggctcga gccggtggtc
      961 aggaacaggt gcgggacgcc cttggcgttg acgtacttga ccaccgcgtt gttggtcggc
     1021 gtgccgaggt tgccgaacag catcagcacg cgatcgtgct cgaccagctt gcgcacctgc
     1081 tcgaccgtct tgggcgggct gtagccgtcg tcgagcgaga cgaaatcgac ccgccggccg
     1141 ccgaccccgc cgtcggcgtt gaccatgtcg aaataggcgc tcatcgcccg gccgatcgcg
     1201 ccataggccg acgccggccc gctgtaggga tgggtcgagc cgatcaggat gcgctcgtcg
     1261 tccgcgccga cgtcatagtc ggcggcgagc gacgccgcgg cggcgagcac ggcgccggcc
     1321 gcgagacctg tcacgatcgc ttttcgccac gccattccgg tccctccgtc tgcggatcat
     1381 agcagccgtc gccgccccgg gcatttgccg ctatgctctg cgccgatctg acgatgccgg
     1441 ggagggcaag catgaaggtc gcggacgcgt tggtcggaat tctccaggcc gagggcgtgg
     1501 agcaggcgtt ctgttatccg ttcagcccgg tcatggacgc gatggcgcgg gccggcctca
     1561 agccgatcgt cacccgccag gagcgggtcg ccggcaacat ggcggacggc gtgtcgcgct
     1621 cgaccaacgg ccgcaagacc ggcacggtca ccgtccaggc gctggcgggc tcggagaacg
     1681 ccttcgccgg catcgcccag gcgttcaccg actcgacgcc ggtgctgttc ctgcccggcc
     1741 atccgggcac ccgcttcatc ggcacctcgc cgaccttcga cagcgccagc aactatgccg
     1801 gcatcaccaa gcacgccgag aagctgctgc tgcccggcga gctctccgcc cagctcaggc
     1861 gcgcgttcac cgcgctgcgc tcgggccggc cgcggccggt gatgcttgag ctgccggcgg
     1921 atgtgtgcgc ggccgagatc gccgcgccgc cggcctacgc gccggtgcgc cggatgcgcg
     1981 ccgcacccga tgcgggcgcg gtcgaagagg ccgccgagcg cctgctcgct gccaaggcac
     2041 ccttgatctg ggccgggcag gggacgctct acgccgaggc gagcgaggaa ctcgtgcgcc
     2101 tcgccgagct cttgggcgcg ccggtcatga ccaccctcca gggcaagagc gcgatgcctg
     2161 agacccatcc gctgtcggcc ggggtcggcg ctctggtctc gaccgcgatg gtacaggcgg
     2221 cgttgcgcga ggccgatgtg ctgctcgcgg tcggcaccag cctgagccgg gcgccgttca
     2281 cgccggcgat cccggacggc aaggacatca tccatgtcac cgtcgatgcc ggcgacatca
     2341 acaaggacta cggcgcggcg ctcggcgtcc cggccgacgc caagctgttc ctcgccgcgc
     2401 tcgccgacga ggtgcgccgg cgcgtgggcg acaacgcccg cgccgaccag tgcgccgagg
     2461 tcgcgcgcaa gctcgccgag acgcgcgtcg cctggcaggc cgagtacgag cccgaattcg
     2521 ccgacgactc ggagccgatc aacggctacc gcatgttccg cgagctgtgg aactcggtgg
     2581 acccggccgc caccatgatt acccatgaat caggcgcctc gcgcgacatc caatgcgtgt
     2641 tctaccgcgc caccaccccg cgcggctatc tgggctgggg gcagtcctcg cagctcggct
     2701 tctcgatcgg cctcgccatg ggcgccaaga tcgccaaccc cgacaagctg gtggtcaacg
     2761 tgatgggcga cggcgccatc ggcatgaccg gcatggacct cgagaccgcg gcgcgcaacg
     2821 gcatcgccat cctgaccgtg gtcaagcacg actcgatctt ctcgggctac gaccgcaaca
     2881 tcccggactc ggtcgcgcgc taccaggcct cgacccagcg cggcgactac gcccagatcg
     2941 cgggcgcgct cggctgccac gccgaacggg tcgagcgggt gggcgagctc cgcccggcgc
     3001 tcgcgcgcgc gatcggtgcg acgcgcgacg gccagccggc gctggtcgac gtgatctgcg
     3061 ccgagacccg caagctgtcg cgcctgccgg cgtgaggttg ttcacagcgc ccggccgcgt
     3121 gcttgcgtag gagcgtgtcc gggtttagac cggcatccga ggcgcgggca agcagggagg
     3181 tgagccatcg cacaaggcgg acaagccaca gcgatcaggt ccgtcgcggg ggcgctgttc
     3241 tacatcgtcc ccgccggcgc caagccggtc acccgcatcg ccgaggaggg caaggggccg
     3301 acccggcgcg aggggcgcta cgaccgccac tccgtcctga tccgcgacgg ccgcgcgatc
     3361 gctggtgagc tcacgctcga ccggcaaggc ttcgtgctgc ttcatcgcga caccgcggtg
     3421 tcggacttct acgaccgcga cgaggtgaag tccgtctact accccgaggt cgagcggctg
     3481 gtgaaggacg cgaccggcgc cgccaaggtg atcgcgttcg accacaacct gcgcgcctcg
     3541 gacgactcga tccgcggcgc gcacggcgtg cgcgagccgg tcaaggtggt gcacaacgac
     3601 tacacgccgc gctcgggccc gcagcgcgtg cgcgacgtga tcggcggcgg cgaggccgag
     3661 gcgctgctgg cgcaccgctt cgcggtcatc aacgtctggc ggccgatccg cgggccggtg
     3721 aagtgcgcgc cgctcgcggt ctgcgatgcc caaagcatcg cgcccggcga cctggtcgcg
     3781 accgacctcg tcta
//
LOCUS       DASWHU010000230        11196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1607408, whole genome shotgun sequence.
ACCESSION   DASWHU010000230 DASWHU010000000
VERSION     DASWHU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607408"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..3544)
                     /gene="metH"
                     /locus_tag="VGB88_08490"
     CDS             complement(41..3544)
                     /gene="metH"
                     /locus_tag="VGB88_08490"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9647521.1"
                     /translation="MTTLAEYLADQVLLLDAGTGGQVQAMDLDVDADFDGNENCTEVL
                     NRTRPDLVRGLHEASLEAGSDAVQANSFGGSPITLGEFALAGEAFDLNRRAAVLAREA
                     VERFAGDGRARFVFGSVGPGTRLASLAQVDYDTLEDALAVQCSGLVAGGVDAILIETV
                     QDLLQAKAAVNGARRAFAEAGRELPIAVQVTIETTGTLLVGADIAAAATVLEALRVPI
                     MGLNCATGPQEMAEHVHWLAEHWPGQISVQPNAGLPELVDGRTRYPLGPEVLAHWLAR
                     FVDEDGVGLIGGCCGTTPAHIAALDAMLRARAGAKRRPAPARRRVHWLPAVSSLYGQV
                     PLRQENAILMIGERCNANGSKLFRAAQVAEDWDACLAIARDQVAEGSHCLDLCAALVE
                     RDEVADMERLVPRLAHAVPAPLVLDSTELKVLARAFRLYGGKPILNSINFEDGEAPAA
                     ARLELARRFGAAVIALTIDETGMAKGADAKLQIAERLADFACGRFGLAPADLLIDPLT
                     FTICTGNDDDRRLGVETLEAIERIARALPESQIVLGLSNISFGLKPAARHVLNSVFLH
                     HARARGMTAAIIHVGKIVALHQIPDHEVAAAEDLIFDRRRPGHDPLHAFIALYEGREA
                     SAARARPRPATVEERLKQRIVDGDRTGLEADLDLALDSYAPLEIINTHLLDGMKVVGE
                     LFGSGRMQLPFVLQSAETMKAAVAHLEPHMERIEGQAKGTIVLATVKGDVHDIGKNLT
                     DIILTNNGYRVVNLGIKQPLANILDAARAERADCVGMSGLLVKSAAIMRENLAEMTRL
                     GLDIPVLLGGAALTRRFVEGDCRTAYAGGPVAYARDAFDGLKLMEAVVAGRFDEVLGA
                     SVPARPAAEAEPAAEPVNGAVSPLLARVAGPDRQAIRLRRAEYAREAAVPTPPFWGAR
                     LIERVPVSGIRPYLNTHTLYQLHWGYKKQGRSFRDFQAWARKELTPILDELIGRCEAE
                     DILVPSAAYGYWRAVADGDDVVLLDEAGARELARFALPRQGRADGLCIADFLRDADSG
                     ESDVIGLQVVTVGARASEVERAWFHADRYRDYLYLHGLGVEIAEAMAEYVHARIRGEL
                     GFGHEDAREIAKLLKQGYRGARYSFGYPACPNLEDQAQLLELLGAGRIGVVLGEEAQL
                     HPEQSTSAIVLHHPRACYFAV"
     gene            complement(3541..4437)
                     /gene="metF"
                     /locus_tag="VGB88_08495"
     CDS             complement(3541..4437)
                     /gene="metF"
                     /locus_tag="VGB88_08495"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX9647522.1"
                     /translation="MASAGPSVSFEFFPPRNQAMAEALWRTVERLAPLGPAFVSVTYG
                     AGGSTRARTHDVVERLRTETSLEPAAHLTCVAASRAEVDAIARRYWHMGVRHLVALRG
                     DPPEGESRFAAHPGGYAGSVELIAGLKRIADFEISAAAYPEPHPDSRGPAADLDHLKR
                     KVDAGAGRLITQYFFDNPTFLAFRDRAVAAGIDAPLVPGILPIANFAQVQRFSARCGA
                     TVPAWLERRFHGLDDDPETRLLVAAATAAEQCRGLRAEGVETFHFYTLNRAELAYATC
                     HLMGLGPVAAAGMAAVSQAPPP"
     gene            complement(4437..5423)
                     /locus_tag="VGB88_08500"
     CDS             complement(4437..5423)
                     /locus_tag="VGB88_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9647523.1"
                     /translation="MLRSLDALLSGLRAAGESTRLRLLALCAEGELTVSELTQILGQS
                     QPRVSRHLKLLCESGLLERHQERTWVFYRLVQPEAGGRLAQSLIALLPRADSTLALDR
                     ERLEAVKRARAERAARYFRDNAARWDEIRALYVPESEVEAAVLAAFGDGPRGDLLDIG
                     TGTGRILELLAPHFERGVGIDQSHEMLDLARVRIEQHGLRHCQVRHGDMYNLPFAGAS
                     FDAVTFHMVLHFADNPTAALAEAARVLRPGGRLVLVDFAPHDQEFLREEHAHRRLGFA
                     ESEVAAWCRTVGLVPARAARLAGAPLTVVVWTAESPAAAGQAGRAPARLRSA"
     gene            5939..6676
                     /locus_tag="VGB88_08505"
     CDS             5939..6676
                     /locus_tag="VGB88_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX9647524.1"
                     /translation="MVLIAAVVSGCATPPAADDPEALAEFEATNDPIEPFNRGVFEFN
                     LFIDRILLKPAAQAYDWLFPAELKSSIHNILSNANEPVNLANATLQGNMERAVTALGR
                     VLINTTLGIGGMIDVASDLGLGPIDEDFGQTLAVWGMGDGPYLMLPLLGPSSVRDGIG
                     RGVDIFLDPMTYVLANSDKDFIGPSIMATTAVDLRARNLERLDEIERTSVDFYAAIRS
                     LYRQRRDDLIRNGEPSDDPFLSNGLEF"
     gene            6694..7344
                     /locus_tag="VGB88_08510"
     CDS             6694..7344
                     /locus_tag="VGB88_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647525.1"
                     /translation="MPRTGALPRRALLAAAIGASLALIAGPASMPARAAPDEAAGFVR
                     VLIDHAIEVLRLPSNEKAQREAGFRDLLVSNFDLPLITRLVVGRYWRQASEDQQREFA
                     SAFEAHIVKVYTSQLGAYEDEHVEIRNVIARTDRDTIVTTDVIRQSDPPLRIDWLVRD
                     DGGGGYKVIDVAAEGVSMMTTKRSEFSSVIAREGLDGLIGRLKDLNARGGTEVGES"
     gene            complement(7354..9039)
                     /gene="ettA"
                     /locus_tag="VGB88_08515"
     CDS             complement(7354..9039)
                     /gene="ettA"
                     /locus_tag="VGB88_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9647526.1"
                     /translation="MASPQYVYHTQGLTKTYAGGRTVLKDISLSFLPGAKIGVLGING
                     AGKSTLLRIMAGDDSDFAGEAWAADGIRVGFLPQEPALEAGRTVGEIVLEGVAATKAL
                     LDEFNTVSAAFAEVADDDRMQALIERQAELQERIEASDAWNLERDVDIALDALRCPPA
                     EAAVDTLSGGERRRVALCRLLLARPDMLLLDEPTNHLDAESVAWLERFLKDYAGTVVA
                     VTHDRYFLDNVAGWILELDRGRGIPWQGNYSSWLEQKRKRLAHEAREAEAMRRQLDHE
                     LEWINASPRARQAKSKARITAYETLAAEAAERERRAGPAQITIPPGPRLGEAVVEAEH
                     LSKGFGVRLLIDDLSFRLPPGGIVGVIGPNGSGKTTLLRLITGAESPDRGALRLGETV
                     KLGYVDQSRDSLAGGNSVWQEISGGRDTIPLGRREVNSRAYVAWFNFRGTDQQKPVGV
                     LSGGERNRVHLAKMLRSGANLLLLDEPTNDLDIETLRALEDALLDFAGSVVAVSHDRW
                     FLDRIATHILAFEGDSRVVWYEGNYADYEADRRRRLGEAADQPHRIRYKPLSR"
     gene            complement(9221..10174)
                     /locus_tag="VGB88_08520"
     CDS             complement(9221..10174)
                     /locus_tag="VGB88_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9647527.1"
                     /translation="MAKAFASQGDVADKTATFAELAPGVYAYTTEGDPNTGVVIGDDG
                     VLVMDTRATPAAARELIAAVRGVTDKPFKYVVLSHYHAVRVLGASAYGAEHVVASEMT
                     RELIAERGRQDYESEVQRFPRLFQGIESVPGLTWPTLTFSERMTIWMGAREVRLMHIG
                     KGHTRGDIIAWLPKEKVCFAGDLVEYGAAPYAGDSYPGEWPATLERLRALRPEKMVPG
                     RGEALTSPAEVNAAIDQTRDFVTTLAKSVADSVAASESLKDAFGRARAALEPRFGRMV
                     IFEHCLPFDVSRAYDEAKGITHPRIWTEARDREMWAALQGA"
     gene            10400..>11196
                     /locus_tag="VGB88_08525"
     CDS             10400..>11196
                     /locus_tag="VGB88_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9647528.1"
                     /translation="MKTYTFPRYAYRPPPELRGGGRHRPVVVVGAGPIGLAAAIDLAL
                     HGVACVVIDDDDTVSIGSRAICWSKRSLEILERLGVAERMMAKGVTWQSGRIFLGERE
                     LYRFDLADEGRQRFPAFINLQQYYVEQYLVERAQEFPEIELRWRNRLAGIEARADGVA
                     LDVDTPDGRYRLDADWVIAADGARSETRRLMGLDFLGRVFEDRFLIADVVMRAGFPKE
                     RWFWFDPPFNPRQSALLHMQADDVWRIDLQLGWDADPDEERKPERVVP"
BASE COUNT         1563 a         3932 c         4097 g         1604 t
ORIGIN      
        1 cctcggcgcg ccagccatga tctccatcaa ggcagggcga tcagaccgcg aagtagcagg
       61 cgcgcgggtg gtggaggacg atcgccgagg tcgactgctc ggggtggagc tgcgcctcct
      121 cgcccagcac cacgccgatc cgccccgcgc ccagcagctc caggagctgg gcctggtctt
      181 cgaggttggg gcaggccggg tagccgaacg agtagcgcgc gccgcggtat ccctgcttca
      241 ggagcttggc gatctcgcgc gcgtcctcgt ggccgaagcc gagctcgccg cggatgcgcg
      301 cgtgcacgta ctcggccatc gcctcggcga tctcgacgcc gaggccgtgc aggtacaggt
      361 agtcgcggta gcggtcggcg tggaaccagg cgcgctcgac ctcggacgcg cgcgcgccga
      421 cggtgaccac ctgcaggccg atgacgtcgc tctcgcccga gtcggcgtcg cgcaggaagt
      481 cggcgatgca cagcccgtcc gcccgcccct ggcgcggcag cgcgaagcgc gccagctcgc
      541 gcgcgccggc ctcgtcgagc agcaccacgt cgtcgccgtc cgcgaccgcc cgccagtagc
      601 cgtaggcggc gctcggcacc aggatgtcct cggcctcgca gcgcccgatc agctcgtcga
      661 ggatcggcgt cagctccttg cgcgcccagg cctggaagtc gcggaagctg cgcccctgct
      721 tcttgtagcc ccagtggagc tgatagagcg tgtgggtgtt gaggtagggg cgaatgcccg
      781 acaccggcac ccgctcgatc agccgcgcgc cccagaacgg cggcgtcggc accgcggcct
      841 cgcgcgcgta ctcggcgcgg cgcaggcgga tcgcctggcg gtccggcccg gcgacgcgcg
      901 caagcagcgg gctcacggca ccgttgaccg gctcggccgc gggctcggcc tccgccgcgg
      961 gccgggcggg cacgcttgcg ccgagcacct cgtcgaagcg cccggcgacc accgcctcca
     1021 tcagcttgag cccgtcgaag gcatcgcgcg catacgccac cgggccgccg gcatacgccg
     1081 tgcggcagtc gccctcgacg aaccggcggg tgagcgcggc gccgcccaag agcaccggga
     1141 tgtcgagccc gaggcgggtc atctcggcca ggttctcgcg catgatcgcg gccgacttga
     1201 ccagcagccc cgacatgccg acgcagtccg cgcgctcggc gcgcgcggcg tcgaggatgt
     1261 tggcgagcgg ctgcttgatg ccgagattga ccacccggta gccgttgttg gtcaggatga
     1321 tgtcggtcag gttcttgccg atgtcgtgga cgtcgccctt gaccgtggcc agcacgatgg
     1381 tgcccttggc ctggccctcg atgcgctcca tgtgcggctc gagatgggcg accgcggcct
     1441 tcatggtctc cgccgactgg agcacgaagg ggagctgcat gcggcctgag ccgaacagct
     1501 cgccgaccac cttcatgccg tcgagcaggt gggtgttgat gatctcgagc ggggcgtagc
     1561 tgtcgagcgc gagatcgagg tcggcctcga gcccggtgcg gtcgccgtcg acgatccgct
     1621 gcttgagccg ctcctcgacc gtcgccgggc gcgggcgcgc gcgcgccgca ctcgcctcgc
     1681 ggccctcgta cagcgcaatg aacgcgtgca gcgggtcgtg gccgggccgg cggcggtcga
     1741 agatcaggtc ctcggcggcg gcgacctcgt ggtccgggat ctggtgcagc gccacgatct
     1801 tgccgacatg gatgatcgcc gcggtcatgc cgcgggcgcg ggcgtggtgc aggaacaccg
     1861 agttgaggac gtggcgcgcc gcgggcttga gcccgaacga gatgttcgac aggccgagca
     1921 cgatctggct ctcgggcagc gcgcgggcga tgcgctcgat cgcctccagg gtctcgacgc
     1981 cgagcctgcg gtcgtcgtcg ttgccggtgc agatggtgaa ggtgagcggg tcgatcagca
     2041 ggtccgcggg cgcgagcccg aagcgcccgc aggcgaagtc ggcgagccgc tcggcgatct
     2101 ggagcttggc atcggcgccc ttcgccatgc cggtctcgtc gatggtcagc gcgatcaccg
     2161 cggcgccgaa gcggcgggcg agctcgagcc tggccgccgc cggcgcctcg ccgtcctcga
     2221 agttgatcga gttcaggatt ggcttgccgc cgtagagccg gaacgcgcgc gccagcacct
     2281 tgagctcggt cgagtcgagc accagcggcg ccggcaccgc atgcgcgagc cgcggcacca
     2341 gccgctccat gtcggcgacc tcgtcgcgct cgaccagcgc ggcgcacagg tcgaggcagt
     2401 gcgagccctc ggccacctgg tcgcgggcga tggcgaggca ggcgtcccag tcctcggcca
     2461 cctgggcagc gcggaacagc ttcgagccgt tggcgttgca gcgctcgccg atcatcagga
     2521 tggcgttctc ctggcgcagc ggcacctggc cgtagagcga ggacaccgcg ggcagccagt
     2581 ggacccgccg cctggccggc gccggccgcc gcttggcccc ggcgcgcgcg cgcagcatcg
     2641 catcgagcgc ggcgatgtgc gccggcgtgg tgccgcagca accgccgatc agcccgaccc
     2701 cgtcctcgtc gacgaaccgg gcgagccagt gggcgaggac ctcggggccg agcggatagc
     2761 gggtgcggcc gtcgaccagc tcgggcaggc cggcgttggg ctggaccgag atctgccccg
     2821 gccagtgctc ggccagccaa tggacgtgct ccgccatctc ctgcgggccg gtggcgcagt
     2881 tgagccccat gatcggcacc cgcagcgcct ccagcacggt cgccgcggcg gcgatgtcgg
     2941 cgccgaccag gagggtaccg gtggtctcga tcgtcacctg gacagcgatc ggcagctcgc
     3001 gcccggcctc ggcgaacgcg cgccgggcgc cgttgaccgc cgccttggcc tgcagcaggt
     3061 cctggacggt ctcgatcagg atcgcatcga cgccgccggc gaccagcccc gagcactgga
     3121 cggcaagcgc gtcctcgagg gtgtcgtagt cgacctgggc gaggctggcc aagcgcgtgc
     3181 cggggccgac cgagccgaac acgaagcgcg cgcggccatc gccggcgaag cgctcgaccg
     3241 cctcgcgcgc gagcacggcc gcgcggcggt tgaggtcgaa cgcctcgccc gccagggcga
     3301 actcgccgag ggtgatcggc gagccgccga agctgttggc ctggaccgca tccgagccgg
     3361 cctcgaggct cgcctcgtgc agcccgcgca ccaggtcggg gcgggtgcgg ttgagcacct
     3421 cggtgcagtt ctcgttgccg tcgaaatcgg cgtcgacgtc gaggtccatc gcctgcacct
     3481 gcccgccggt gccggcatcg agcagcagga cctgatccgc gaggtactcg gcgagcgtgg
     3541 tcatggcggc ggcgcctgcg acaccgcggc catgccggcg gcggcgaccg ggccgagccc
     3601 catcaggtgg caggtggcgt aggcgagctc ggcgcggttg agggtgtaga agtggaaggt
     3661 ctcgacaccc tcggcgcgga gcccgcggca ttgctcggcg gctgtcgccg cggcgaccag
     3721 caggcgcgtc tcggggtcgt cgtcgaggcc atggaagcgg cgctcgagcc acgccggcac
     3781 ggtcgcgccg cagcgcgcgc tgaaccgctg gacctgggcg aagttggcga tcggcaggat
     3841 tcccggcacc aggggtgcgt cgatgccggc ggcgacggcg cggtcgcgaa acgcgagaaa
     3901 ggtcgggttg tcgaagaagt actgggtgat caggcggccg gcgccggcgt cgaccttccg
     3961 cttgaggtgg tcgaggtcgg cggcgggacc gcgcgagtcg gggtgcggct cgggataggc
     4021 ggcggcgctg atctcgaagt cggcgatgcg cttgaggccg gcgatcagct cgaccgagcc
     4081 ggcatagccg ccgggatgcg cggcgaagcg gctctcgccc tcgggcgggt caccgcgcag
     4141 cgccaccagg tggcgcacgc ccatgtgcca gtagcggcgc gcgatcgcgt cgacctcggc
     4201 gcggctcgcc gcgacgcagg tcaggtgcgc ggctggctcg aggctggtct cggtgcgcag
     4261 cctctcgacc acgtcgtggg tgcgcgcccg cgtgctgccg ccggcgccgt aggtgaccga
     4321 gacgaaggcc gggccgagcg gcgccagccg ctcgaccgtg cgccacagcg cctccgccat
     4381 cgcctgattg cgcggcggga agaactcgaa cgacacggac ggcccggcgc tcgccatcac
     4441 gcgctcctca gcctcgccgg cgcgcgcccg gcctggcccg ccgcggccgg cgactcggcg
     4501 gtccacacca ccactgtcag cggcgcgccg gcgaggcggg cggcgcgcgc cggcaccagg
     4561 ccgacggtcc ggcaccaggc tgcgacctcg ctctcggcaa agcccaggcg gcggtgcgcg
     4621 tgctcctcgc gcaggaactc ctggtcatgg ggcgcgaagt cgaccagcac cagccgcccg
     4681 ccgggccgca agacgcgcgc ggcctcggcc agcgcggcgg tcgggttgtc ggcgaaatgc
     4741 agcaccatgt ggaaggtgac ggcatcgaac gaggcgccgg caaacggcag gttgtacatg
     4801 tcgccgtggc ggacctggca gtggcgcagg ccgtgctgct cgatgcgcac acgcgccagg
     4861 tcgagcatct cgtgcgactg gtcgatgccg acgccgcgct cgaagtgcgg cgccagcagc
     4921 tcgaggatgc gcccggtgcc ggtgccgata tcgagcaggt cgccgcgcgg cccatcgccg
     4981 aacgccgcca gcaccgcggc ctcgacctcg ctctcgggca cgtagagcgc gcggatctcg
     5041 tcccagcgcg ccgcgttgtc gcggaaatac cgcgccgccc gctcggcgcg cgcgcgcttg
     5101 accgcctcca gccgctcgcg gtcgagcgcc agggtcgagt ccgcgcgcgg cagcagcgcg
     5161 atcaggctct gcgccagccg gccgcccgcc tcgggctgga ccagccgata gaacacccag
     5221 gtccgctcct ggtggcgctc aagcagcccg gattcgcaca gcagcttcag atggcgcgag
     5281 accctgggct ggctttggcc caggatctgg gtcagctcgc tcaccgtcag ctcgccctcg
     5341 gcgcacagcg cgagcaggcg cagacgagtc gattcgccgg cggcgcgcag gccgctgagc
     5401 agggcgtcga gggagcggag caaggacgcg ttcatttctt gcctatataa acatattttt
     5461 atgttttaat gcaacgcctc gatcgacgcc gcaccgggcc tgccgggtcg aacggccggt
     5521 ccagggaacg cagcgcgaac ctggggcgtg gccgttgctg ccgggcggct gggacatatt
     5581 tacaacagcg gggggcgggt gcttaggtgg gcactgcgcg gactggcgca acaggggggc
     5641 tcatgctact cggattcaat gcgattcgcc cgagtcgcgc cggcgcggcg cggccatggc
     5701 gagcgagtgc tgtagcgcaa ttgccggggg cgtgcgcgag acaatgtctt gcggtcttgg
     5761 attgtccgac gccgcaggcg acgctggcaa ttcgaccgcg tcgccggctc atgctcggct
     5821 gatggtggcc aggggggcac cgcgggtggc gcgggagatg ccatcggccg cgactggtcg
     5881 tgcgcccgcg gccgtgcggc gcggccgggg agcggcgcat gccggcggcg cgctcgctat
     5941 ggtgctgatc gcggccgtcg tgtcaggctg cgcgacgccg ccggcggcgg acgatccgga
     6001 ggcgttggcg gagttcgagg cgaccaacga tccgatcgag ccgttcaacc gcggcgtgtt
     6061 cgagttcaac ctgttcatcg accggatcct gctcaagccg gcggcccagg cctacgactg
     6121 gctgttcccg gccgagctga agtcctcgat ccataacatc ctgagcaacg ccaacgagcc
     6181 ggtgaacctt gccaatgcca ccctgcaggg caacatggag cgcgccgtca ccgcgctcgg
     6241 gcgggtgctg atcaacacca cgctcggcat cggcggcatg atcgacgtcg ccagcgacct
     6301 ggggctcggg ccgatcgacg aggacttcgg gcagaccctc gcggtctggg gcatgggcga
     6361 cggcccctac ctgatgctgc cgctgctcgg cccctcgagc gtgcgcgacg gcatcggccg
     6421 cggcgtggat atctttctcg acccgatgac ctatgtgctc gccaactcgg acaaggactt
     6481 catcggaccg tcgatcatgg cgaccacggc ggtcgacctg cgcgcgcgca acctggagcg
     6541 gctggacgag atcgagcgca cctcggtcga cttctatgcc gcgattcgca gcctctaccg
     6601 ccagcggcgc gacgacttga tccgcaacgg cgaaccgagc gatgatccgt tcctgtccaa
     6661 tggcctggaa ttttgacgag gacgacgact gtcatgccta gaacgggtgc tctgccgcgg
     6721 cgcgcgctgc tggccgcggc gatcggcgcg tctttggcgc tcatcgccgg ccccgcctcg
     6781 atgccggcgc gcgccgcgcc cgacgaggcc gccggcttcg tgcgcgtgct gatcgaccac
     6841 gcgatcgagg tgctgaggtt gccgagcaac gagaaggcgc agcgcgaggc cgggttccgc
     6901 gatcttctgg tcagcaactt cgacctgccg ctgatcaccc gcctggtggt cgggcgctac
     6961 tggcgccaag ccagcgagga ccagcagcgg gagttcgcga gcgcgttcga ggcgcacatc
     7021 gtcaaggtct acacctcgca gctcggcgcc tacgaggacg agcacgtcga gatccgcaat
     7081 gtgattgcgc gcaccgaccg cgacaccatc gtcaccaccg acgtgatccg gcagtccgac
     7141 ccgccgttgc gcatcgactg gctggtgcgc gacgacggcg gcggcggcta caaggtcatc
     7201 gacgtcgccg ccgagggggt cagcatgatg accaccaagc gctcggagtt ctcgtcggtg
     7261 atcgcgcgcg agggcctgga cggcttgatc ggccggctca aggacctcaa cgcccgcggc
     7321 ggcaccgagg tcggcgagtc ctgaccgcgc cggtcagcgg ctgagcggct tgtagcggat
     7381 gcggtgcggc tggtcggccg cctcgcccag gcggcgcctg cggtcggcct cgtagtcggc
     7441 gtagttgccc tcgtaccaga ccacccggct gtcgccctcg aaggcgagga tgtgggtggc
     7501 gatgcggtcg agaaaccagc ggtcgtggct gaccgcgacc accgagccgg cgaagtcgag
     7561 gagcgcgtcc tcgagcgcgc ggagcgtctc gatgtcgagg tcgttggtcg gctcgtcgag
     7621 cagcagcagg ttggcgcccg acctgagcat cttggccagg tgcacccggt tgcgctcgcc
     7681 gcccgacagg acgccgaccg gcttctgctg gtcggtgccg cggaagttga accacgccac
     7741 gtaggcgcgg ctgttgacct cgcgccggcc gagcggaatc gtgtcgcggc cgcccgagat
     7801 ctcctgccac accgagttcc cgcccgccag gctgtcgcgg ctctggtcga catagccgag
     7861 cttgaccgtc tcgccgagcc tgagcgcgcc gcggtcgggc gactcggcgc cggtgatcag
     7921 ccgcagcagc gtggtcttgc ccgagccgtt ggggccgatc acgccgacga tgccgcccgg
     7981 cggcaggcgg aacgacaggt cgtcgatcag gagccgcacg ccgaagccct tggagaggtg
     8041 ctcggcctcg accaccgcct cgccgagccg gggcccgggc gggatcgtga tctgggcggg
     8101 gccggcgcgg cgctcgcgct cggcggcctc ggcggcgagg gtctcgtagg cggtgatccg
     8161 ggccttgctc ttggcctggc gggcgcgcgg gctggcgttg atccactcga gctcgtgatc
     8221 gagctggcgg cgcatggctt cggcctccct cgcctcatgg gcgaggcgct tgcgcttctg
     8281 ctccagccac gacgagtagt tgccctgcca ggggatgccg cggccgcggt cgagctcgag
     8341 gatccagccg gcgacgttgt cgaggaagta gcggtcgtgg gtgaccgcga ccacggtgcc
     8401 ggcatagtcc ttgaggaagc gctccagcca ggccaccgac tcggcgtcca gatggttggt
     8461 cggctcgtcc agcagcagca tgtcggggcg ggcgagcaag agccggcaca gcgccacccg
     8521 ccggcgctcg ccgcccgaga gcgtgtcgac cgccgcctcg gcgggcgggc agcgcagcgc
     8581 atcgagcgcg atgtcgacgt cgcgctcgag gttccacgcg tcgctcgcct cgatccgttc
     8641 ctgaagctcg gcctggcgct cgatcagcgc ctgcatccgg tcgtcgtccg cgacctcggc
     8701 gaaggcggca ctcaccgtgt tgaactcgtc gagcagcgcc ttggtcgcgg cgaccccctc
     8761 gagcacgatc tcgcccaccg tgcgcccggc ctccagtgcc ggctcctggg gcaggaagcc
     8821 gacccggatg ccgtccgcgg cccaggcctc gccggcgaag tcggaatcgt cgccggccat
     8881 gatcctgagc agggtcgact tgccggcgcc gttgatgccg agcaccccga tcttggcgcc
     8941 cggcaggaac gacagcgaga tgtccttcag caccgtccgc ccgccggcgt aggtcttggt
     9001 caggccctgg gtatggtaga cgtactgggg agaagccacg cttagccgcg cccgcctcgc
     9061 attctcgaaa attcctgtcc gcgccgcccc ccctcaccct tccctctccc cccaaatgtg
     9121 ggggagaggg ttgcgcaggg ttggcgcggc gacagccgcg gctttacccg aagctgggtg
     9181 aagggggagc ggggccgagc gcgacatgcc gtcgtcgccc tcaggcgccc tggagcgccg
     9241 cccacatctc gcgatcgcgg gcctcggtcc agatgcgcgg atgggtgatg cccttggcct
     9301 cgtcgtaggc gcggctcacg tcgaacggca ggcagtgctc gaagatcacc atccggccga
     9361 agcgcggctc gagcgcggcg cgcgcgcgcc cgaaggcgtc cttgagcgac tcgctcgcgg
     9421 cgacgctgtc ggcgacgctc ttggcgagcg tcgtgacgaa gtcgcgggtc tggtcgatcg
     9481 cggcgttgac ctcggcaggg ctggtcaacg cctcgccgcg gccgggcacc atcttctcgg
     9541 gcctgagcgc gcgcagccgc tccaaggtcg ccggccactc gcccggatag gaatcgccgg
     9601 catagggcgc cgcgccgtac tcgaccaggt cgccggcgaa gcacaccttc tccttcggca
     9661 gccaggcgat gatgtcgccg cgggtgtggc ccttgccgat atgcatcagc cggacctcgc
     9721 gcgcgcccat ccagatggtc atgcgctcgg agaaggtgag cgtcggccaa gtgagccccg
     9781 gcaccgactc gatcccctga aacaggcgcg ggaagcgctg gacctcggac tcgtaatcct
     9841 gccggccgcg ctcggcgatc agctcgcggg tcatctcgct ggcgacgacg tgctcggcgc
     9901 cgtaggcgct ggcgccgagc acgcgcaccg cgtggtaatg gctcagcacc acatatttga
     9961 acggcttgtc ggtgacgccc cgcaccgccg cgatcagctc gcgcgcggcc gccggggtcg
    10021 cgcgcgtgtc catcaccagc acgccgtcgt caccgatcac cacgccggtg ttggggtcgc
    10081 cctcggtggt gtaggcgtag acgccggggg cgagctcggc aaacgtcgcg gtcttgtcgg
    10141 cgacatcgcc ctgggacgcg aatgccttgg ccatgcccgg ccctcctggt ggtgtgcggc
    10201 tgcggcggaa gcggcgcctg tatagcaaag ccgtcctcag tcgcaaagga aaggcgggca
    10261 cgcggaggcg cggagggcgc ggagaagaaa taggcattgc cgcccccctc accgcgtcct
    10321 ccgcggctcc gcgtgagccc aattccgggc cgccctggcg gcctcccggg cgatctggta
    10381 tctgagcggc tcaggcgcca tgaagaccta tacgttcccg cgctatgcgt atcggccgcc
    10441 gcccgagctt cgcggcggcg gcaggcatcg cccggtggtg gtggtcggcg ccgggccgat
    10501 cggcctcgcc gcggcgatcg acctcgcgct gcacggcgtc gcctgcgtgg tgatcgacga
    10561 cgacgacacg gtcagcatcg gcagccgcgc gatctgctgg tcgaagcgct cgctcgagat
    10621 cctggagcgc ctcggcgtgg ccgagcgcat gatggccaag ggggtgacct ggcagagcgg
    10681 gcgcatcttc ctgggcgagc gcgagctgta ccgcttcgac ctcgccgacg agggccgcca
    10741 gcgcttcccc gccttcatca acctccagca gtattacgtc gagcaatacc tggtcgagcg
    10801 cgcgcaagag ttcccggaga tcgagctgcg ctggcgcaac cggctggccg gaatcgaggc
    10861 gcgcgccgac ggcgtcgccc tcgacgtcga caccccggac ggccgctacc ggctcgatgc
    10921 cgactgggtg atcgccgccg acggcgcgag gagcgagacg cgccgcctca tgggcctcga
    10981 cttcctgggc cgggtgttcg aggaccgctt cctgatcgcc gacgtggtga tgcgcgcggg
    11041 cttccccaag gagcgctggt tctggttcga cccgccgttc aacccgcgcc agtcggcgct
    11101 cttgcacatg caggcggacg acgtgtggcg gatcgatctc cagctcggct gggacgccga
    11161 ccccgacgag gaacgcaaac ctgagcgcgt ggtgcc
//
LOCUS       DASWHU010000231         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3427312, whole genome shotgun sequence.
ACCESSION   DASWHU010000231 DASWHU010000000
VERSION     DASWHU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427312"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VGB88_08530"
     CDS             <1..1027
                     /locus_tag="VGB88_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647529.1"
                     /translation="GGRNGIRALIAAFGVLAVATAPAARAETVLRLSNWVPPSHLISR
                     DILAPWAGEVERVTEGRVRVDILKAPLGKPPAHFDIARDGLADVTVGVHGYTPGRFVL
                     TEIAELPFLSDSAEALSVAYWRVHERYLAKAGEHKGVHLLALWAHGPGHIFNARRPIR
                     AIDDLAGLKFRVGGGLVSEISDALGTVAVQAPATQAYELVANGVADGIYFPVESVTFF
                     NLTEPLRYATLIPGGLYNSSFFLVINEASWQALGAADRAAIMAVSGEALARRAGRTWD
                     AADRAGLEAMTAAGTALEVADAAMLEALKAQLAFARRDWVDRASDRGVDGALALAALE
                     AEIARLK"
     gene            1024..1497
                     /locus_tag="VGB88_08535"
     CDS             1024..1497
                     /locus_tag="VGB88_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9647530.1"
                     /translation="MTVGRNAARTAGRALAIAAGALLLAMLALTVADVLGRYLLARPI
                     PGAFELTQVMLALLIFCGLPLVSARDGHVAVSLIDPWLRPGAARWRDRLVWALGALVC
                     ATIAWRLAALGARLADYGDRFEFIGLPRAAVAYPMSALAAAAALVLLAKALGGRR"
     gene            1507..2799
                     /locus_tag="VGB88_08540"
     CDS             1507..2799
                     /locus_tag="VGB88_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9647531.1"
                     /translation="MAAVAAGFAALFLLILLRLPIAFAMMLVGAGGVAALVGWLPALA
                     MVQQVTFDTGLSYELSVVPLFVLMGNLVTRAGLSDELYAAAHAWLGHRRGGLAMATVV
                     ACGGFSAICGSSLATAATMARVAMPPMRRYGYADSLAAGAVAAGGTLGILIPPSVILV
                     LYGIMTGQDIGALFAAGLLPGLLGIAGYVGAIAVVTWRDPALGPPAERTGWPARWRAT
                     ARVWGVAVLFALVMGGIYGGVFTPTEAAGIGAGGGLAFALARRALDWPALLDVLVRSA
                     ETTAMLFFVLIGALMFANFVNLAGLPAALSDFVAGLDAPPLAVIAAIVAIYLVLGSVL
                     ESLSMVMLTVPVFYPLVGDLGLDLIWFGIVVVVVTEISLITPPIGLNVFVLRGVMPEL
                     SLATAFRGVTPFWIADIFRLAAIVLVPGIALLLPALIR"
     gene            2842..3984
                     /locus_tag="VGB88_08545"
     CDS             2842..3984
                     /locus_tag="VGB88_08545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9647532.1"
                     /translation="MSHDFIVIGAGIAGASAACELARHGKVALLEREDRPGYHSTGRS
                     AAFYTLNYGNEIIRTLTDLSGDFFRDPPDGFAESPLIVRHATVTIARADQEASFRDNL
                     RAARASAGRIDELDPAAAERLIPILRPGYAAFAHLEPDSYFMDVALIHGGFLKSLAAR
                     GGRLVCGAEVGGLKRARDAWRVTTKAGAFEAPVVVNAAGAWADDIARLAGAAPAGLQP
                     MRRTAIIVAAPAGIDIAACPLVIDTDEQFYFRPEAGKVLASPADETPSVPCDAQPEEA
                     DVAVTVDRLERATTLSVRRVEHRWAGLRSFVADRAPVVGPDPAAEGFVWLAGQGGYGI
                     MTSPAMARAAAALAAGSAWPAELSTRGITPARLAPGRASLAATVRA"
     gene            4007..4357
                     /locus_tag="VGB88_08550"
     CDS             4007..4357
                     /locus_tag="VGB88_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6614 family protein"
                     /protein_id="HEX9647533.1"
                     /translation="MDIYHGWFNLKDGVGDADFAARFRAYMAHLEAEGLIAGWRLTRR
                     KLGLGPAFLPEFHIMIETDGLAQLDAAFALVSGRAEPVEGLHHAVNSAVQDVVFALYR
                     DFPDPGRKTGSERF"
     gene            complement(4365..>4742)
                     /locus_tag="VGB88_08555"
     CDS             complement(4365..>4742)
                     /locus_tag="VGB88_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647534.1"
                     /translation="RISHVVAAVENESSCEAEVTSRRFIIQTPISNGSNATVSFADNA
                     IVVSGTGASARDGAGNAEAWFDAAQPIALDFTQPGGQASQAQGTLPLYHSVVIDDRDH
                     RFTVQAADTRGFVTVTEQVCAYP"
BASE COUNT          543 a         1741 c         1711 g          747 t
ORIGIN      
        1 gggcggcaga aacggcatcc gggcgctgat cgccgccttc ggggtgctgg cggtcgcgac
       61 ggcgcccgcg gcccgcgccg agacggtgct gaggctgtcc aactgggtgc cgccgagcca
      121 cctgatcagc cgcgacatcc tggcgccgtg ggcaggggag gtcgagcgcg tcaccgaggg
      181 ccgggtgcgc gtcgacatcc taaaggcgcc gctcggcaag ccgccggcac atttcgacat
      241 cgcccgcgac ggcctcgccg atgttaccgt cggcgtccac ggctacacgc cagggcggtt
      301 cgtgctgacc gagatcgccg agctgccgtt cctgagcgac agcgccgaag cgctgtcggt
      361 ggcctattgg cgggtgcatg agcgctatct cgccaaggcg ggcgagcaca agggcgtgca
      421 cctgctggcg ctgtgggcgc acggccccgg gcacatcttc aacgcccggc gcccgatccg
      481 cgccatcgac gacctcgccg ggctaaagtt ccgggtcggc ggcgggctgg tcagcgagat
      541 ctcggacgcg ctcggcaccg ttgccgtcca ggcgccggcg acccaggcct acgagctggt
      601 cgccaacggc gtcgccgacg gcatctattt cccggtcgag tcggtgacct tcttcaacct
      661 gaccgagccg ctgcgctacg ccacgctgat tcccggcggg ctgtacaact cgagcttctt
      721 cctggtgatc aacgaggcga gctggcaggc actgggcgcc gccgaccggg ccgcgatcat
      781 ggccgtctcc ggcgaggcac tggcgaggcg cgcggggcgc acctgggacg ccgccgatcg
      841 cgccgggctc gaggcgatga ccgccgccgg caccgcgctc gaggtcgccg atgcggcgat
      901 gctggaggcg ctcaaggcgc agctcgcgtt cgcccgccgc gactgggtcg accgcgcctc
      961 cgaccgcggc gtcgacggcg cgctggcgct ggccgcgctc gaggccgaga tcgcccgcct
     1021 caagtgaccg tcgggcgcaa cgccgcgcgc actgcggggc gcgcgctcgc catcgccgcc
     1081 ggtgcgctgc tgcttgccat gctcgccctc actgtcgccg acgtgctcgg ccgctacctg
     1141 ctcgcgcggc cgatcccggg cgcgttcgag ctcacccagg tgatgcttgc cctgctcatc
     1201 ttctgcggcc tgccgctggt ctcggcgcgc gacggccatg tcgcggtgtc gctgatcgac
     1261 ccgtggctga ggccgggtgc cgcgcgctgg cgcgaccgcc tggtctgggc gctgggcgcg
     1321 ctggtgtgcg cgaccatcgc ctggcggctc gccgcgctcg gcgcgcgcct cgccgactac
     1381 ggcgaccggt tcgagttcat cggcctgccg cgcgccgccg tcgcctatcc gatgagcgcg
     1441 ctggccgccg ccgccgccct ggtgctgctc gccaaggcgc tcggcggccg gcgctgaccg
     1501 ctggccatgg cggcggtcgc ggccggcttc gccgcgctgt tcctgctgat cctgctgcgc
     1561 ctgccgatcg cgtttgcgat gatgctggtc ggcgccggcg gggtcgcggc cctggtcggc
     1621 tggctgccgg cgctggccat ggtccagcag gtcaccttcg acaccgggct gtcctatgag
     1681 ctgtcggtgg tgccgctgtt cgtgctgatg ggcaacctgg tcactcgcgc cggactgtcg
     1741 gacgagctct acgccgccgc ccacgcctgg ctcggccatc gccgcggcgg gctggcgatg
     1801 gcgaccgtgg tcgcctgcgg cggcttcagc gcgatctgcg gctcgagcct ggcgaccgcg
     1861 gcgaccatgg cacgcgtcgc catgccgccg atgcgccgct acggctatgc cgactcgctc
     1921 gctgccggcg cggtcgccgc cggcggcacc ctcggcatcc tgatcccgcc gagcgtgatc
     1981 ctggtgctct acggcatcat gaccggacag gacatcggcg ccttgttcgc cgccggcctg
     2041 ctgccgggcc tgctcggcat cgccggctat gtcggcgcga tcgcggtcgt gacctggcgc
     2101 gacccggcgc tcggcccgcc ggcggagcgc actggctggc cggcacgatg gcgcgcgacc
     2161 gcgcgggtgt ggggggtggc ggtgctgttc gcgctggtca tgggcggcat ctacggcggg
     2221 gtgttcacgc ccaccgaggc ggccggcatc ggcgccggcg gcggcctagc cttcgcgctc
     2281 gcccggcgcg ccctcgactg gcccgcgctc ctggacgtgc tggtgcgctc ggcggagacc
     2341 acggcgatgc tgttcttcgt cctgatcggg gcgctgatgt tcgcgaactt cgtcaacctg
     2401 gcgggcctgc cggcggcgct gtccgacttc gtcgcggggc tcgatgcgcc gccgctcgca
     2461 gtcatcgccg ccatcgtcgc gatctatctg gtgctcggca gcgtgctcga gagcctgtcg
     2521 atggtgatgc tgaccgtgcc ggtattctac cccctcgtcg gcgatctcgg cctcgacctg
     2581 atctggttcg gcatcgtcgt ggtggtggtg accgagatca gcctgatcac gccgccgatc
     2641 gggctcaacg tgttcgtcct gcgcggggtg atgccggagc tgtcgctggc gaccgcgttc
     2701 cgcggcgtga ccccgttctg gatcgccgac atctttcggc tcgccgcgat cgtgctggtg
     2761 ccgggcattg cgctcctgtt gcccgcgctg atccgctgag ggccttgtga aggggaagcg
     2821 gagcggctat gtcatgcggc catgagccat gacttcatcg tcatcggcgc cggcatcgcc
     2881 ggcgcctcgg ccgcctgcga gctcgcccgc cacggcaagg tggcgctgct cgagcgcgag
     2941 gaccgccccg gttatcactc gaccgggcgg tccgccgcct tctacacgct caactatggc
     3001 aatgaaatca ttcgcacgct caccgacctg agcggcgact tcttccgcga cccgccggac
     3061 ggcttcgccg aatcgccgct gatcgtgcga catgcgaccg tgaccatcgc ccgcgccgac
     3121 caggaggcga gctttcgcga caacctgcgc gcggcgcgtg cctcggccgg caggatcgac
     3181 gagctcgacc cggccgcggc cgagcgcctg atcccgatac tgaggcccgg ctacgccgcc
     3241 ttcgcccatc tcgagcccga ctcgtatttc atggatgtcg ccctgatcca cggcggcttt
     3301 ctcaaatccc tcgccgcgcg cggcgggagg ctggtctgcg gcgccgaggt cggcgggctg
     3361 aagcgcgcac gcgacgcttg gcgcgttacc accaaagccg gcgcgttcga ggcgccggtg
     3421 gtggtcaatg ccgcgggcgc ctgggccgat gacatcgcgc gcctcgccgg cgccgcgccg
     3481 gcggggctcc agccgatgcg ccgcaccgcg atcatcgtcg ccgcgcccgc gggcatcgac
     3541 atcgccgcct gcccgctggt gatcgacacc gacgagcagt tctatttccg gcccgaggcc
     3601 ggcaaggtgc tggcgagccc ggccgacgag acgccgtctg tgccctgcga cgcgcagccc
     3661 gaggaggcgg acgtcgcggt cacggtcgat cgcctggagc gcgcgaccac cttgagcgtg
     3721 cgccgggtcg agcacaggtg ggcgggcctt aggagcttcg tcgccgaccg ggcgccggtg
     3781 gtcgggcccg accccgcggc cgagggcttc gtctggctcg ccggccaggg cggctacggc
     3841 atcatgacct cgccggcgat ggcgcgcgcc gccgccgcgc tcgccgcggg cagcgcgtgg
     3901 ccggccgagc tctccacccg cggcatcacg ccggccaggc tcgcgccggg ccgcgcgagc
     3961 cttgccgcga cggtgcgcgc gtaaggcggg cgcggaggcc tgggcggtgg acatctatca
     4021 cggctggttc aacctcaagg acggcgtcgg cgatgccgac ttcgccgccc gcttccgggc
     4081 ctacatggcg cacctcgagg ccgagggcct gatcgccggc tggcggctga cccgccgcaa
     4141 gctcggcctc ggccccgcct tcctgcccga gttccacatc atgatcgaga ccgacggcct
     4201 ggcccagctc gatgccgcgt tcgccctcgt ctccggccgc gccgagccgg tcgagggcct
     4261 ccaccacgcg gtcaacagcg cggtccagga cgtggtgttc gcgctctacc gcgacttccc
     4321 cgaccccggc cgcaagaccg ggtcggagcg cttctaggcc gtggtcacgg gtaggcgcag
     4381 acctgctcgg tgacggtgac gaagccgcgc gtgtcggcgg cctggaccgt gaacctatgg
     4441 tcgcggtcgt cgatcaccac cgagtggtag agcggcaagg tgccctgggc ctggctcgcc
     4501 tggccgccgg gctgggtgaa gtcaagggcg atcggctggg cggcatcgaa ccaggcttcc
     4561 gcgttgccgg cgccgtcgcg cgcggacgcg ccggtgcccg aaaccacgat cgcgttgtcg
     4621 gcgaacgaca ccgtggcgtt gctgccgttc gagatcggcg tctggatgat gaacctgcgg
     4681 ctggtcacct cggcctcgca gctgctctcg ttctcgaccg ccgcgaccac gtgggagatg
     4741 cg
//
LOCUS       DASWHU010000232         5030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2357531, whole genome shotgun sequence.
ACCESSION   DASWHU010000232 DASWHU010000000
VERSION     DASWHU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357531"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1882)
                     /locus_tag="VGB88_08560"
     CDS             complement(392..1882)
                     /locus_tag="VGB88_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9647535.1"
                     /translation="MKVSTTHLLACAAMAAALAASPASAQQDIESRILDLEQQIKLLK
                     TQLEAQVKQAQETADQAAAKAMDDSGVKVKMKGPAPTFESADGRYTMSITGRVHWDAA
                     WYDQEDNSQVIGGMVVNPTVVPDLNSGTNLRRARLGVEGIIEKDWEYNLVFDAGDSSA
                     GNFQIDEIFLGYNGIKPLRLRVGRHKTPNGFDELTSSNDIPFIERSSTNNAALGPAGA
                     KHVGLSANAHGDFWWAGLGVFGADWNQQTDDEQVSVNGRLAIAPLRMGNEYALHIGAS
                     GYHVFEPNQQNGANTITFSDRPNIRVDSNQWISASTTSADSAYMVGLEAAGQYQNLWL
                     NGEVNWFGYDEYFNPNDLRSQRGEQDFMGYYLQAGWVITGEPRPYSMSKAAWSGIKPA
                     NPFSIENAGWGAWELVGRYDAVDLNDQENSFASDGTFLGTRGGEQEAYTVGLNWWVND
                     YIAFKLNYIHVDVDRIGGTANCSTGVNCPSAGDTFDVFALRTQVKW"
     gene            2150..3673
                     /locus_tag="VGB88_08565"
     CDS             2150..3673
                     /locus_tag="VGB88_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9647536.1"
                     /translation="MSGTPSGTRRRGTAVLRVADMYRADAAAVAAGVPSLALMENAAA
                     AIVREVRRRWSPRPTLVLCGPGNNGGDGFGVAIGLAAARWPVTAALLGERARLSGDAA
                     VMAGRWPGPVTPFDPALIEGASLVVDAVFGAGLARPVDGRARAVLEALAARGCEVVAV
                     DVPSGVHGDTGAVMGAAAPARLTVTFFRPKPGHLLLPGRALCGELVVADIGIPDAALD
                     DIAPPLAVNGPRLWGARLPRPAAGGHKYDRGHAVVAGGATTTGAARLAARAALGVGAG
                     LVTVAAPAAAFAIYAGALTSVMVTAIASEGDFDAVLADRRKNALLIGPGHGVGEATRA
                     RVLATLAAGKRCVLDADALTVFADDREALAAAIARAEVPVVLTPHDGEYARLFGFEGD
                     RLQRALAAAEASGAVVALKGPDTVVAAPDGRAAITADAPPELASAGTGDVLAGLIVGL
                     LAQGADGFDAACMGTWLLGALGRAAGPGLVSERLVDHLPGVLAAVRREAGASAPGDG"
     gene            3666..>5030
                     /locus_tag="VGB88_08570"
     CDS             3666..>5030
                     /locus_tag="VGB88_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEX9647537.1"
                     /translation="MAEAGATLLDRTLRRLRRGWHELSLSGPRSLADTLSRKLDGADL
                     ARVRSAVTDCLEGRGGEVSALSRAAALGEAYLILDATGRGRFLNLLATEFGCDRTQVD
                     AAAQALLSARDGDRAGAERALRMALSPRRFALFRQLGALPQGVKFLVELRAELERLSL
                     DEDVRRSLSEELRDLLSSWFDYGFLELRRITWASPADLLERLMAYEAVHEIRSWTDLK
                     NRLDSDRRCFAFFHPRMPGEPLIFIEVALTSGLADSVEALLDETAAATDPAQADTAIF
                     YSISNAQRGLAGISFGGFLIKRVVDLLRAEFPKLKTFATLSPMPEFRAWLADCVATAP
                     KSIAALVSDDDIAVLRETLDRNWPANPEQVAALREPLIRLGAHYLLAEKRNGRASDPV
                     AHFHLSNGARVERLNFLADRSPKGMRESAGLMVNYLYKLDEIEANHEAYGAEGKIRAS
                     SQLRG"
BASE COUNT          637 a         1736 c         1853 g          804 t
ORIGIN      
        1 ccgaccgcgg cgctgaacgc ggcgaggccc gcgcctttca ggaacccgcg ccggtccggc
       61 tgccagcgcc ggccgaagac cagcgcgtcg gcacgctccg ggtcgtcgtc gtaaagctcg
      121 cacaggccac gcttggtatc cgtcatcgcg ccctccggag atgttgttgt tgttcgcccg
      181 gcgggacatc ttgcgagata cgcccggcac tgtccatgct gccgcgcacg cgcagtgcgg
      241 agcaaaaaac agggtcgcgg gcggactggt acccgcgacc ctcaggggtt cggacggcac
      301 gcgcgggggc tggtcggcgt gcggtccgag ccggaacctc cccgcggaca ctcaccgcat
      361 cgcaggacgg ttccggttca tcggcgcacg cctaccattt cacctgggtg cggagcgcga
      421 acacgtcgaa ggtgtcgccg gccgaggggc agttgacgcc ggtcgagcag ttggcggtgc
      481 cgccgatgcg gtcaacatcg acgtggatgt agttgagctt gaacgcgatg tagtcgttga
      541 cccaccagtt caggccgacg gtgtaggcct cctgctcgcc gccgcgggtg ccgaggaagg
      601 tgccgtcgga ggcgaacgaa ttctcctgat cgttgaggtc gacggcgtcg tagcggccga
      661 ccagctccca cgcgccccag ccggcattct cgatgctgaa cgggttggcg ggcttgattc
      721 cgctccacgc cgccttggac atgctgtagg ggcgcggctc gccggtgatc acccagccgg
      781 cctggaggta gtagcccatg aagtcctgct cgccgcgctg gctgcgcaag tcgttcgggt
      841 tgaagtattc gtcgtagccg aaccagttga cctcaccgtt gagccagaga ttctggtact
      901 ggccggcggc ctcgagcccg accatgtagg cactgtcggc cgaggtggtg ctggcgctga
      961 tccattggtt cgagtcgacg cggatgttcg gccggtcgga gaaggtgatg gtgttggcgc
     1021 cgttctgctg gttcggctcg aagacgtggt agccggaggc gccgatgtgg agcgcatact
     1081 cgttgcccat gcgcagcggc gcaatggcca ggcggccgtt gacgctgacc tgctcgtcgt
     1141 cggtctgctg gttccagtcg gcgccgaaca cgcccaagcc ggcccaccag aagtcgccgt
     1201 gcgcgttcgc gctcaggccg acatgcttgg cgccggccgg cccgagggcg gcgttgttgg
     1261 tcgaggagcg ctcgatgaac gggatgtcgt tcgagctggt cagctcgtcg aacccgttcg
     1321 gcgtcttgtg ccgcccgacc ctcaggcgca gcggcttgat gccgttatag cccaggaaga
     1381 tctcgtcgat ctggaagttg ccggccgagc tgtcgccggc gtcgaagacc aggttgtact
     1441 cccagtcctt ttcgatgatg ccttcgacgc cgaggcgcgc gcggcgcaag ttggtgccgc
     1501 tgttgaggtc gggcaccacc gtcggattga ccaccatgcc gccgatgacc tggctgttgt
     1561 cctcttggtc gtaccaggcc gcgtcccagt gcacccggcc ggtgatcgac atggtgtagc
     1621 ggccgtcggc ggactcgaag gtcggggcgg ggcctttcat cttgaccttg accccgctgt
     1681 cgtccatcgc ctttgcggcg gcctggtccg ccgtctcctg cgcctgcttc acctgcgcct
     1741 cgagctgcgt cttgagcagc ttgatttgct gctcgaggtc gaggatgcgg gattcgatgt
     1801 cctgttgcgc cgaggcggga gaggcggcca aggccgcggc catcgccgcg caggcgagca
     1861 ggtgcgtggt agagaccttc atgtcgggat gtccttctcc gtggcgtttt gtcgtggccg
     1921 gtcacgggtc ggacgacccg ccccccgttt ggcgccggga taggccgcgc gtgttacatc
     1981 ccaagtggcg gtttgatgtc gttttgatga caagggggga gacaaacccc aatgttttca
     2041 acttgctgac gagtcgtgcc ggagacgcgg cgggcgctgt tgcggcgtcg caacggacgg
     2101 tcgcgggttt tctgttgcca agggcaagcc gcctcatagg atcgccgcca tgagcgggac
     2161 cccgagcggc acgcgccggc gaggtacggc ggtgctccgt gtcgccgaca tgtatcgcgc
     2221 cgacgccgcc gcggtggcgg cgggcgtgcc gagcctggcc ctgatggaga acgccgccgc
     2281 cgcgatcgtg cgcgaggtcc gccgccgctg gagcccgcgc ccgaccctgg tgctgtgcgg
     2341 gcccggcaac aacggcggcg acggcttcgg ggtcgcgatc gggctcgcgg cggcgcgctg
     2401 gccggtgacg gcggccttgc tcggcgagcg cgcgcgactg agcggcgacg ccgcggtgat
     2461 ggccgggcgc tggccagggc cggtcacgcc gttcgatccg gccctgatcg agggcgcgtc
     2521 gctggtcgtc gacgcggtgt tcggcgccgg gctcgcgcgc ccggtcgacg gccgagcgcg
     2581 cgcggtgctc gaggcgctcg cggcgcgcgg gtgcgaggtg gtggctgtcg acgtgccctc
     2641 gggagtccac ggcgacaccg gcgcagtgat gggcgcggcg gcgccggccc ggctgacggt
     2701 caccttcttc cggccgaagc ccgggcacct gctgctgccc ggacgcgcgc tgtgcggcga
     2761 gctggtggtc gccgacatcg gcatccccga cgccgcgctc gacgacatcg ccccgccgct
     2821 cgcggtcaac ggcccgcggc tgtggggcgc caggctgccg cgcccggccg cgggcgggca
     2881 caagtacgac cgcggccacg ccgtcgtcgc cggcggcgca accaccaccg gcgcggcacg
     2941 gcttgcggcg cgcgccgcgc tcggggtcgg cgccgggctg gtcaccgtcg ccgcgcccgc
     3001 agcggcgttc gcgatctatg ccggggcgtt gacctcagtc atggtgacgg cgatcgcgag
     3061 cgagggggat ttcgacgccg tgctcgccga tcggcgcaag aacgcgctgc tgatcggccc
     3121 cggccacggc gtcggcgagg ccacccgcgc gcgcgtgctg gctacccttg cggcgggcaa
     3181 gcggtgcgtg ctcgacgccg atgcgctgac cgtgttcgcc gacgaccgcg aggcgctggc
     3241 cgcggcgatc gcgcgcgccg aagtgccggt ggtgctgaca ccgcacgacg gcgagtacgc
     3301 gcgcctgttc ggcttcgagg gcgaccggct tcagcgcgcg ctcgccgccg ccgaggcgag
     3361 cggcgcggtg gtcgcgctca aggggcccga cacggtggtc gcggccccgg acgggcgcgc
     3421 ggcgatcacc gccgatgcgc cgcccgagct tgcctcggcc ggcaccggcg acgtgctcgc
     3481 cggcctgatc gtcggcctgc tcgcccaggg cgcggacggc ttcgatgccg cctgcatggg
     3541 cacttggctg ctgggcgcgc ttggccgcgc cgccgggccg gggctggtca gcgagcggct
     3601 ggtcgatcat ctgcccggcg tgctcgctgc ggtgcgccgc gaagccggag cgtcggcgcc
     3661 gggtgatggc tgaagcgggc gcgaccctgc tcgaccgcac gctgcgccgg ctgcggcgcg
     3721 gctggcacga gctgtcgctg agcggcccgc ggtcgctcgc cgatacgctg tcgcgcaagc
     3781 tcgacggcgc cgacctcgcg cgcgtgcgca gcgcggtgac cgactgcctc gaagggcgcg
     3841 gcggcgaggt ctccgcgctg tcgcgcgcgg cggcgctggg cgaagcctat ctgatcctcg
     3901 atgcgaccgg gcgcggacgg ttcctgaacc tgctcgcgac cgagttcggc tgcgaccgca
     3961 cgcaggtcga cgccgccgcc caggccttgc tgtcggcgcg cgacggcgac cgcgccgggg
     4021 ccgagcgggc gctgcgcatg gcgctgtcgc cgcgccggtt cgccttgttc cgccagctcg
     4081 gcgcgctgcc acagggggtc aagttcctgg tcgagctgcg cgccgagctc gaacggctgt
     4141 cgctggacga ggacgtgcgc cggagcctgt cggaggagct tcgcgacctg ttgtcgtcgt
     4201 ggttcgacta cggcttcctc gagctgcgcc ggatcacctg ggcgtcgccc gccgacctgc
     4261 tcgaacgcct gatggcctat gaggcggtgc acgagatccg ctcctggacc gacctcaaga
     4321 accggctcga ctcggaccgc cgctgcttcg ccttcttcca cccgcgcatg ccgggcgagc
     4381 cgctgatctt catcgaggtc gcgctcacca gcggccttgc cgacagcgtc gaggcgctgc
     4441 tcgacgagac cgccgcggcg accgatccgg cccaggccga caccgcgatc ttctactcga
     4501 tctcgaacgc ccagcgcggg ctcgccggga tcagcttcgg cggcttcctg atcaagcgcg
     4561 tggtcgacct gctgcgcgcg gagttcccga agctcaagac cttcgccacc ctgtcgccga
     4621 tgccggagtt cagggcgtgg ctcgcggact gcgtcgcgac cgcgcccaag tcgatcgcgg
     4681 cgctggtgtc cgatgacgac atcgcggtgc tgcgcgagac cttggaccgg aactggcccg
     4741 ccaacccgga gcaggtggcg gcgttgcgcg agcccctgat caggctcggc gcccactacc
     4801 tgctggcgga gaagcgcaac ggccgggcga gcgacccggt ggcccatttc cacctgtcga
     4861 acggcgcccg cgtcgagcgg ctcaacttcc tcgccgaccg ctcgcccaag ggcatgcgcg
     4921 aatccgccgg gctgatggtc aactacctgt acaagctcga cgagatcgag gccaaccacg
     4981 aggcctatgg ggccgagggc aagatcaggg catcgtcgca gctgcgcggc
//
LOCUS       DASWHU010000233         7475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1611174, whole genome shotgun sequence.
ACCESSION   DASWHU010000233 DASWHU010000000
VERSION     DASWHU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611174"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1306)
                     /locus_tag="VGB88_08575"
     CDS             complement(89..1306)
                     /locus_tag="VGB88_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9647538.1"
                     /translation="MPLYRYKAVSRAGEMIEGELAGADKGAVIERLHQLGHTPIRADE
                     VSGGGGGWLARPLLGAGRIGRREIAVFTREMATLLGAGLTLERALEILAGLSDHEPTR
                     RMLARLLETIRSGASFADALAAQGAAFPRYYVSMVRAGEAGGALEQVLARLGDYMERA
                     QALRESVRSALIYPTIVAVMAGGAIVMLLTVVLPQFTPLFEDAGAALPPSTRIVVAVG
                     EAVEDYGWAGLAVLLALVLLIRQQLASPALRARWDALLLRLPLAGDLIAKAETARFSR
                     ILATLLSNGVPMLSALAIVKDTLANRVTAKAIEDIGTSLKEGEGLAEPLAKARHFPAL
                     SAQLVRVGEETGQLEQMLHKVADIYDQEVRRAVDRMLALLVPGMTLALGGLIALIIGS
                     ILSAMFSVYDIPL"
     gene            complement(1331..3049)
                     /gene="gspE"
                     /locus_tag="VGB88_08580"
     CDS             complement(1331..3049)
                     /gene="gspE"
                     /locus_tag="VGB88_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX9647539.1"
                     /translation="MNDSKPTVADVEASFEDRLGRALVAAGKLQAAGLERARRVAQSG
                     PSLHLVLTRLGLVSEGDMAEALAHELGLALATRADYPETPLLEGSAKFLKESRVMPLA
                     ESPEALTLAMADPLDSYAIRAVELLADKPVVTWVGLPADIEAAIERAYGGGKSSIEEI
                     SDQLEGEAGEITEQDVERLRDLASEAPVIRLVNMLITRAVESRASDIHIEPFENQLKV
                     RYRVDGVLQEVDSPPSKLRAAIISRIKLMAKLNIAERRLPQDGRIKLAVRGKEIDLRV
                     ATLPTMHGESVVLRVLDRGSVRLDFASLGFRGKALERLLGVLERPNGIVLVTGPTGSG
                     KTTTLYAALARLNSPEKKILTVEDPIEYQLEGVNQLQVKPQIGLSFANVLRSFLRQDP
                     DIIMVGEIRDLETAQIAVQAALTGHLVLSTLHTNNAASTITRLLDMGVDDYLLTATVN
                     GIAAQRLVRTLCPRCRAAFPALPELVAELRLDRFTDGGAVTLHRAVGCEACNGTGYHG
                     RTNILEMLVMSDGLRARVLNKAEAGDIQQAAVAEGMVTMQEDGLRKALAGETTVEEIL
                     RVTREV"
     gene            complement(3070..3711)
                     /locus_tag="VGB88_08585"
     CDS             complement(3070..3711)
                     /locus_tag="VGB88_08585"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX9647540.1"
                     /translation="MGAGGEGWAVAATAVAAALLAPAVDRLAGGGWRRPRPAAMAAFA
                     LAGALLAGWAAATLGAPLWLSCALALVLLALAEVDRRTLALPDLVTLPLVAAGLAVAA
                     ALAPARWTDHVTGALAGFAALAAIGWGYARLRGREGLGLGDAKLLAAAGAWLGWQALP
                     GVVTIAAAGALLAALAGRAFGRPLSATDPVPFGPTLALGFWLSWLYGPITIGW"
     gene            3793..4413
                     /locus_tag="VGB88_08590"
     CDS             3793..4413
                     /locus_tag="VGB88_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9647541.1"
                     /translation="MKLFGSPLSPFVRKVRAAIHEMGLDSRIAFTAVTMTPVAPDAVL
                     NRANPLGKIPALELDDGSSLYDSPVICEYLDGVHDRPRLLPPAGPARWQALRRQALAD
                     GIMDAGVLVRYEIAVRPEALRWDDWIAAQKGKIARALDSLEAEAGALGGGFDIGRIAI
                     ACALGYLDFRFAADRWRDRRPALAAWFETAAKRPSISSTAPELAAT"
     gene            complement(4410..5654)
                     /locus_tag="VGB88_08595"
     CDS             complement(4410..5654)
                     /locus_tag="VGB88_08595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9647542.1"
                     /translation="MADPDGASGRDGGRASLAARAALDARFLARVALIWIAAAVATHV
                     WYVIDRTRAGLTDGRRWPLGDDFINTWSAARLALEGRAGEIYDLAAFHAFQVSVVGPD
                     LQLYHYSYPPLLLVLSLPLAPLAYVPALAAWTLFGLAALVAALRCALSWRAALLVGLA
                     TPAVLINDISGQNGLVTAALLGGGLGLLDRRPHVAGVLLGLLAFKPQLGVLVPVALAA
                     GGHWRAFAAAAVTVLALVGASLAAFGVDLWADYAAQADFLRREILEDAEGLWHRFASV
                     FVAARTLGAGVAAAYALQAAAALVALAAVVAVWRSRTPQPVKGAVLVLAALLATPYVQ
                     DYDLVVATFAVVWLLQAEAPAIGDSAARRLVGVLAALFVLPLLAASVGKLIGVSFAAA
                     VIALGLAAALWLWRRGPAAAAG"
     gene            5881..6921
                     /locus_tag="VGB88_08600"
     CDS             5881..6921
                     /locus_tag="VGB88_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9647543.1"
                     /translation="MIFRQMFDSTSCTYTYLIASRRGGEAVIVDPVLDKVDRYLKLLE
                     ELDLKLVKAADTHVHADHITGLGALRDRTRCVTVIGEQSKTDVVSMRVADGDRIEIEG
                     IALEAMYTPGHTDDSYCYRMSDRVMTGDTLFIRGTGRTDFQHGDAVAQYHSIFDRLLT
                     LPEETLVYPGHDYKGDTVSTIGEEKAFNPRLQVDSVEQYVEIMNSLNLPNPKMMDVAV
                     PANLKVGLSQDEIEAHGWSLAPEQAFALVGGAAAILIDLRDRGERTRHGEIPGSVRAP
                     YGALEANLGAHGLLRQLAGSGKRLVFYCAYGERSAMAVQAAHEAGIADAIHVRGGLDQ
                     WRKRGGPIEPAP"
BASE COUNT         1133 a         2738 c         2646 g          958 t
ORIGIN      
        1 agcaccacca gcagctcgag cagcgtgaag ccgccgtcac gccgggtgcc gcgggcgcgc
       61 ggaccacgac ggcgttgcgc cagggtcgtc acagcgggat gtcgtagacg ctgaacatgg
      121 cgctcaggat cgagccgatg atcaacgcaa tcaagccccc aagtgcgagc gtcattcccg
      181 gcaccagcag cgccagcatg cggtcgaccg cgcggcggac ctcctggtcg tagatgtcgg
      241 ccaccttgtg cagcatctgc tcgagctggc cggtctcctc gccgacccgc accagctggg
      301 cgctgagggc gggaaagtgc cgggccttgg cgagcggctc ggccagcccc tcgccttctt
      361 tcaggctggt gccgatgtcc tcgatcgcct tggccgtgac gcggttggcg agcgtgtcct
      421 tgacgatggc gagcgcgctc agcatcggca cgccgttgct gagcagcgtc gccaagatgc
      481 ggctgaaccg cgccgtctcg gccttggcga tcaggtcgcc ggcgagcggc aggcgcaaca
      541 ggagcgcgtc ccagcgcgcg cgcagggcgg gactggcgag ctgctggcgg atcaggagca
      601 ccagcgcgag cagcactgcg agccccgccc agccgtagtc ctcgaccgcc tcgccgaccg
      661 cgaccacgat ccgggtcgac ggcggcagcg cggcgcccgc gtcctcgaac agcggcgtga
      721 actggggcag caccacggtc agcagcatca cgatcgcgcc gccggccatg accgcgacga
      781 tggtcggata tatcagcgcc gagcgcacgc tctcgcgcag cgcctgggcc cgctccatgt
      841 agtcgccgag gcgcgccagc acctgctcga gcgcgccgcc ggcctcgccc gcgcgcacca
      901 tgctgacgta atagcgggga aaggcggcgc cctgtgccgc cagcgcatcg gcgaacgagg
      961 cgccggaccg gatcgtctcg agcaggcgcg ccagcatccg acgagtcggc tcgtggtccg
     1021 atagcccggc caggatctcg agcgcccgct cgagggtgag cccggcgccg agcagggtcg
     1081 ccatctcgcg ggtgaacacg gcgatctcgc gccgtccgat gcgcccggcg ccgagcagcg
     1141 ggcgggcaag ccacccgccg cccccgccgc tgacctcgtc ggcgcggatc ggggtgtggc
     1201 cgagctggtg caggcgctcg atcaccgcgc ccttgtcggc gccggcaagc tcgccctcga
     1261 tcatctcgcc ggcgcgcgag accgccttgt agcgatacag cggcatggcc cggccggctc
     1321 cgccctcgcc ctagacttcc cgagtgacgc gcaggatttc ctcgaccgtg gtctcgccgg
     1381 ccagcgcctt gcgcaggccg tcttcctgca tggtgaccat gccttcggcg accgccgcct
     1441 gctggatgtc gccggcctcg gccttgttca gcacccgcgc gcgcaagccg tccgacatga
     1501 ccagcatctc gaggatgttg gtgcggccgt gatagccggt gccgttgcac gcctcgcagc
     1561 cgacggcgcg gtggagagtc accgcaccgc cgtcggtgaa ccggtcgagg cgcagctcgg
     1621 cgaccagctc gggcaaggcg gggaaggcgg cgcggcagcg cgggcacagc gtgcgcacca
     1681 ggcgctgggc ggcgatgccg ttgacggtgg cggtcaacaa atagtcgtcg acgcccatgt
     1741 cgagcaggcg ggtgatcgtg ctggcggcgt tgttggtgtg caaggtcgac agcaccaggt
     1801 gcccggtcag cgccgcctgg accgcgatct gcgcggtctc gaggtcgcgg atctcgccga
     1861 ccatgatgat gtccgggtcc tggcgcagga acgagcgcaa gacgttggcg aagctgagcc
     1921 cgatctgcgg cttgacctgg agctggttaa ccccttcgag ctggtactcg atcgggtcct
     1981 cgacggtgag gatcttcttc tcgggcgaat tcagccgcgc cagcgcggcg tagagcgtgg
     2041 tggtcttgcc gctgccggtc gggccggtga ccagcacgat gccgttcggc cgctcaagca
     2101 cgcccaacag ccgctccagc gccttgccgc ggaagccgag gctggcgaag tcgaggcgca
     2161 cgctgccgcg gtcgagcacg cgcagcacca cgctctcgcc gtgcatggtc ggcagcgtgg
     2221 cgacgcgcag gtcgatctcc ttgccgcgca ccgcgagctt gatgcgcccg tcctggggca
     2281 ggcggcgctc ggcgatgttg agcttggcca tcagcttgat gcgcgagatg atcgccgcgc
     2341 gcagcttgct cggcggcgag tccacctcct gcaggacgcc gtcgacccgg tagcgcacct
     2401 tgagctggtt ctcgaacggc tcgatgtgga tgtcggaggc gcggctctcg accgcgcgcg
     2461 tgatcagcat gttgaccagg cggatgaccg gcgcctcgct ggccaggtcg cgcaggcgct
     2521 cgacgtcctg ctcggtgatc tcgccggcct cgccctcgag ctggtcgctg atctcctcga
     2581 tcgacgactt gccgccgcca taggcgcgct cgatcgcggc ctcgatgtcg gcgggcagcc
     2641 cgacccaggt gacgaccggc ttgtcggcca gcagctcgac cgcgcggatg gcgtaggaat
     2701 cgagcgggtc ggccatcgcc aaggtcagcg cctcgggcga ctcggcgagc ggcatcaccc
     2761 gcgattcctt caggaacttg gcgctgccct cgagcagcgg cgtctccggg tagtcggcgc
     2821 gcgtcgccag cgccaggccg agctcgtgcg ccagcgcctc ggccatgtcg ccctcggaga
     2881 ccaggccgag gcgggtcagc accagatgca gcgacggccc gctctgggcg acccggcggg
     2941 cgcgctcgag ccccgcagcc tgcagcttgc cggccgccac cagggcgcgc ccgagccggt
     3001 cctcgaacga cgcctcgacg tctgcgacgg tgggcttgga gtcgttcacc acgtcctctc
     3061 gactttagtc taccacccga tcgtgatcgg gccataaagc cagctcagcc agaacccgag
     3121 cgcgagggtg gggccgaacg gcaccgggtc ggtggccgac agcggccggc cgaatgcgcg
     3181 cccggcgagc gccgccagca gcgcgccggc ggcggcgatg gtgaccacgc cgggcagcgc
     3241 ctgccagccg agccaggcgc cggcggcggc gagcagcttg gcatcgccga ggccgagccc
     3301 ctcgcgcccg cgcaggcgcg catagcccca gccgatcgcg gccagcgcgg caaagccggc
     3361 gagcgctcct gtgacatggt cggtccagcg cgccggcgcc agtgccgcgg ccaccgccag
     3421 gccggccgcg accagcggca gggtcaccag gtcgggcagc gcgagggtgc gccggtcgac
     3481 ctcggcgagc gccagcagga ccagggcgag cgcacagctc agccacagcg gcgcgcccag
     3541 ggtggccgcc gcccagccgg cgagcaacgc gcccgcgagc gcgaacgccg ccatcgccgc
     3601 cgggcgcggc cggcgccagc cgccgcccgc cagccggtcg accgcgggcg cgagcagcgc
     3661 cgccgcgacc gccgtcgccg ccaccgccca gccctcgccg cccgcgccca tcgactgcct
     3721 caacccggcc ctggtcccgc tcgtcggaat cgacataatg caccaccgca ggaccacagg
     3781 gaggataccg gggtgaagct gttcggcagt ccgctgtcgc cgttcgtgcg caaggtgcgc
     3841 gcggcgattc acgaaatggg cctcgattcg cggatcgcgt tcacggcggt gacgatgacg
     3901 ccggtggcgc cggacgcggt gctcaaccgg gccaacccgc tcggcaagat cccggcgctt
     3961 gagctcgacg acggctcaag cctgtacgac tcgccggtga tctgcgagta cctcgacggc
     4021 gtccacgacc ggccgcggct gttgccgccg gcggggccgg cgcgctggca ggcgctgcgc
     4081 cgccaggcgc tcgccgacgg catcatggac gccggcgtcc tggtccgcta cgagatcgcg
     4141 gtccggcccg aggcgttgcg ctgggacgac tggatcgccg cccagaaggg caagatcgcg
     4201 cgcgcgctcg attcgctcga ggccgaggcg ggcgcgctcg gcggcggctt cgacatcggc
     4261 cggatcgcga tcgcctgcgc gctcggttat ctcgacttcc gcttcgccgc cgaccgctgg
     4321 cgcgaccgcc gcccggcgct cgcggcctgg ttcgagaccg ccgccaagcg cccgtcgatc
     4381 agctcgaccg cgccggagct cgcggccact taacctgcgg cggcggcggg gccgcggcgc
     4441 cacagccaca gcgccgcggc caggccgagc gcgatcaccg cggcggcgaa gctcaccccg
     4501 atcagcttgc cgaccgaggc cgcgagcagc ggcagcacga acagcgccgc cagcacaccg
     4561 accagccgcc gcgccgcgct gtccccgatc gccggcgcct ccgcctgaag cagccagacc
     4621 acggcgaagg tcgcgaccac caggtcgtag tcctgcacgt acggggtcgc gagcagggcg
     4681 gcgagcacca gcaccgcgcc cttcaccggc tgcggcgtgc gcgaccgcca gaccgcgacc
     4741 accgcggcca gcgcgaccag cgccgccgcc gcctgaagcg cgtacgccgc cgccaccccg
     4801 gcgccgagcg tgcgcgcggc cacgaacacc gaggcgaacc ggtgccacag gccctcggcg
     4861 tcctcgagga tctcgcgccg caggaaatcg gcttgcgccg cgtagtcggc ccagaggtcg
     4921 acgccgaacg cggcgaggct cgccccgacc agcgccagca cggtgacggc ggcagcggcg
     4981 aaggcgcgcc agtggccgcc agcggcgagc gcgaccggca ccagcacgcc gagctgcggc
     5041 ttgaacgcga gcaggccgag caggaccccg gcgacgtgcg gcctgcggtc gaggagcccg
     5101 agcccgccgc ccagcagcgc cgcggtgacc aagccgttct ggccgctgat gtcgttgatc
     5161 agcaccgccg gcgtcgccag gccgaccagc agcgccgcgc gccacgaaag cgcacagcgc
     5221 agcgccgcga ccagcgcagc aagcccaaag agagtccacg ccgccagcgc cggaacgtat
     5281 gccagcggcg cgagcggcaa gctcaggacc agcagcagcg gcgggtagga gtagtggtag
     5341 agctgcaggt cggggccgac caccgagacc tggaaggcgt ggaacgccgc caggtcgtag
     5401 atctcgccgg cccgcccctc gagcgcgagg cgggccgccg accaggtgtt gatgaagtcg
     5461 tcgccgagcg gccagcggcg gccgtcggtg agcccggcgc gggtgcggtc gatgacgtac
     5521 cagacgtggg tagcgaccgc cgcggcgatc cagatcagtg ccacgcgggc gaggaagcgc
     5581 gcatcgagcg cggcgcgcgc ggcgaggctc gccctacctc cgtcccgccc gctcgcgccg
     5641 tccgggtccg ccatcggacc cgatcctatc gccccgcgcc gtgcgaagcg accagtcgag
     5701 aacggactca cgcggaggcg cggccttcga gacgccctct tgcgagggct cctcaggctg
     5761 aagagcgccg aaggcgcgtc tcgaagcctc cgcgcctccg cgtgcctaat cgtcgctcgc
     5821 gatgttatga ttgcgctcag acacagccgg caggcggggc gcagacctgg aggccgtttc
     5881 atgatcttcc gccagatgtt cgacagcacc tcctgcacct acacctacct gatcgccagc
     5941 cgccgcggcg gcgaggcggt gatcgtcgat cccgtgctcg acaaggtcga ccgctacctg
     6001 aagctcttgg aggagctcga cctgaagctg gtcaaggcgg ccgacaccca cgtccatgcc
     6061 gaccacatca ccggcctcgg cgcgctccgc gaccgcaccc gctgcgtcac cgtgatcggc
     6121 gagcagagca agaccgacgt ggtctcgatg cgcgtcgccg acggcgaccg gatcgagatc
     6181 gagggcatcg cgctcgaggc gatgtacacc cccgggcaca ccgacgattc gtactgctac
     6241 cggatgagcg accgggtgat gaccggcgac accctgttca tccgcggcac tggccgcacc
     6301 gacttccagc acggtgatgc ggtcgcccag taccactcga tcttcgaccg cctgctcacg
     6361 ctgccggagg agacgctggt ctatccgggc cacgactaca agggcgacac ggtcagcacc
     6421 atcggcgagg agaaggcgtt caacccgagg ctccaggtcg attcagtcga gcagtacgtc
     6481 gagatcatga acagcctcaa cctgcccaac ccgaagatga tggacgtcgc ggtgccggcg
     6541 aacctcaagg tcgggctgtc gcaggacgag atcgaggccc acggctggtc gctcgcgccc
     6601 gagcaggcgt tcgccctggt cggcggcgcc gctgcgatcc tgatcgacct gcgcgaccgc
     6661 ggcgagcgca cccgccacgg cgagatcccg ggctcggtgc gcgcgccgta tggcgcgctc
     6721 gaggcgaacc tcggcgcgca cggcctcttg cgccagctcg ccggcagcgg caagcggctg
     6781 gtgttctact gcgcctacgg cgagcgctcg gcgatggcgg tgcaggcggc ccacgaggcg
     6841 ggcatcgccg atgccatcca cgtcaggggc ggcctcgacc agtggcgcaa gcggggcggc
     6901 ccgatcgagc cggcgccata gcgcccatgc gcaccggccc ggcgaccgag gtcaagcggc
     6961 gcctggccgc ggtcccggac gcagcccggc gcgagccggt gatcatccgc cggcgccggc
     7021 gcgcgcgccg gccgaggacg agctcgccga tatggtagcg gacgcggacg gcggctgacc
     7081 gccggcgtgc cggcgtcccg acccgcgact cgcggcgtcc tcaccctcgg cgcaagactg
     7141 tttcgcgctc cctcggccga tcgtggtaac ggccggcatc agaccggctc tccgtaatat
     7201 gtgagaatgc atcgcattcg ctcttgactt cgagtcgtga ttgcgaatta gtcgcagtgc
     7261 gagtgtgagc cattcgcata ggtagccccc cactgcctcg gcgggggggg cgagctgtca
     7321 acgaaggact cgtgggtggg attcgatgcg cagactcgcg cccgccgccg aggaggtggg
     7381 gggctagata tgcgaatggc tcacactcgc actgcgacta attcgcaatc acgactcgaa
     7441 gtcaagagcg aatgcgatgc attctcacat attac
//
LOCUS       DASWHU010000234         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3093058, whole genome shotgun sequence.
ACCESSION   DASWHU010000234 DASWHU010000000
VERSION     DASWHU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093058"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGB88_08605"
     CDS             complement(<1..222)
                     /locus_tag="VGB88_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647544.1"
                     /translation="MAHRRRRAGLLAAVAGLSAATAAAGDGFRTDAPAMLAGLGGWSV
                     EPVFTVGETIGDYTPPGVLDGIGAFARADG"
     gene            complement(250..>4104)
                     /locus_tag="VGB88_08610"
     CDS             complement(250..>4104)
                     /locus_tag="VGB88_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9647545.1"
                     /translation="ISASLAAPAPDAPLTFAGALTLNDEPVSAKVTVTNFGDALAGNR
                     FTLAAALDSKRVTASYDGAVQTRPLPGLDGALALDVASVGALLSWLGRPLPADQPDPG
                     PLALAAKLVAEGQSVALTEATIRGEGLDATAFGSIDAGGEVTKVAFRLEGESLDIDRY
                     LPPRPAGAQALPAEPAPPPAAPAGDQGAPAAKADMFAAVPDAPLDLTALDAIDADIAI
                     KLGALSAMGYRAGPLSVSLKAAGGSASFEIAELGLYGGTVEGRLAVAPAETAGEITLD
                     ADFGIDKVAVGELAEAAGAAVPMVGIASGALVAKSRGASPRALAENLSGRVELDLGGV
                     EATDEALRAVSELALAVDLPGIDAAPAVNVELVYNGQRVRLAATSDPLPKVLGEDRFD
                     LDLSVDSALVTASYKGAVQQRPVPALDGAFALDAGSVGKLAGWLGRPLPEGQPDPGPL
                     SVRAAVAADGAKVVLSEASIAGEGLNARAHGSFERGATVQKVDFTLEADSLDIDRYLP
                     PPAPVEQAVAEQAAAEPAAAAAPAPAAQADLFAAIPDRPFDLAHMRTMEGQVSVRIGA
                     LRAMGYEVGPVVLSGAMAGGVHQMRIEQLGLYGGEVTAQVAADGSGDELSVDAEVTVD
                     KVAVARLAEAAGAALPVAGVASGRVSATGRGANARALAESLAANVDVALAGIETSDEA
                     FAGLSELSLKASLTGIDAGPAIDASAVYKGEQVALAAAVDPLPKVLGEDRFGVDLSVD
                     SALVTASYKGAVQSRPTPGLDGGLAVETASVGRLLAWLGQPLPAGQPDPGALAVKATL
                     GASGPVVTLSEATVEGAAFRARADGRFDSAGAVPVFNAALTIDRLDANAFLPPPAEPA
                     PPSGGGGGGGGAPPPAPAAAPGDWSDEPFDMAALRGADGEVTIDARDVRYRDLVIDAL
                     KAKAAVAGGVLTATVDELKLAGGTVAGAATVDASGDALSLDYRADASGLQALPLLSAF
                     ADLRRLTGTMAFAAKGAGHGRSQRELVGSLAGDGMVRFTDGAITGFNLAAGLRQARSL
                     GLDTGAGETQQTDFSELGGTYTITNGVIDNRDLKMLAPLVRVDGGGLVPMPPRMVDYK
                     VTAKLVGSLEGQGGQDALAGLPIPITVRGPWHQPATEVDWESVFREAAKDPARIAAMP
                     QQLLDAAKDFGVALPIPGLSGGAEGGIGKLLEGVLPPGGDQGGDQGGVGRLIEGLLPK
                     AAPAPEGAQGAAPAPSSPLKLLEGVLQPPGAAPAPAPAPAPAPAPEVAPAPAPAPAPA
                     PAETEAAPPNPVDVIRGLLKRD"
BASE COUNT          496 a         1579 c         1538 g          491 t
ORIGIN      
        1 gccgtcggcg cgcgcgaacg cgccgatgcc gtcgagcacg ccgggcgggg tgtaatcgcc
       61 gatcgtctcg ccgaccgtga acaccggctc gaccgaccag ccgccgaggc cggccagcat
      121 cgccggcgcg tcggtgcgga agccgtcccc ggccgccgcc gtcgccgccg acaggccggc
      181 gacggccgcg agcagccccg cccggcgtcg gcgatgcgcc aattcgtccc cctcgaaccc
      241 gggcccgggt cagtctctct tgagcaggcc gcggatcacg tcgaccgggt tcggcggcgc
      301 ggcctcggtc tcggccggcg ccggagcggg cgccggtgcc ggtgccggcg cgacctcggg
      361 cgcgggtgca ggcgccggcg cgggggctgg agccggagcg gcgcccggcg gttgcagcac
      421 gccttccagc agcttgagcg ggctcgacgg cgcgggcgcg gccccttgcg cgccttcggg
      481 cgccggcgcc gccttcggca gcaggccctc gatcaaccgg ccgacgccgc cctggtcgcc
      541 accctggtcg ccgccgggcg gcagcacgcc ttcgaggagc ttgccgatgc cgccttcggc
      601 gccgccggac agcccgggga tcggcagcgc gacgccgaag tccttggccg cgtccagcag
      661 ctgctggggc atcgcggcga tgcgcgccgg gtccttggcc gcctcgcgga agacgctctc
      721 ccagtcgacc tcggtcgccg gctggtgcca ggggccgcgg acggtgatcg ggatcggcag
      781 gccggcgagc gcgtcctgcc cgccctgccc ttcgaggctg ccgaccagct tggcggtgac
      841 cttgtagtcg accatgcgcg gcggcatcgg caccaggccg ccgccgtcga cgcgcaccag
      901 cggcgcaagc atcttgaggt cgcggttgtc gatcactcca ttggtgatgg tgtaggtgcc
      961 gccgagctcg ctgaagtcgg tctgctgggt ctcgccggcg ccggtgtcga ggccgaggct
     1021 gcgggcttgc ctgagccccg ccgccaggtt gaagccggtg atcgcgccgt cggtgaagcg
     1081 gaccatgccg tcgccggcga gcgagccgac cagctcgcgc tggctgcgcc cgtggcccgc
     1141 gcccttggcc gcaaaggcca tggtgccggt gagccggcgc agatcggcga acgccgacaa
     1201 cagcggcagc gcctggagcc cgctcgcgtc ggcccggtag tcaaggctga gcgcatcgcc
     1261 cgaggcatcg accgtcgccg cccccgccac cgtgccgccg gccagcttga gctcgtcgac
     1321 ggtcgcggtc agcacgccgc ccgcgactgc cgctttcgcc ttgagcgcat cgatgaccag
     1381 gtcgcgatag cgcacgtcgc gcgcgtcgat cgtgacctcg ccgtcggcgc cgcgcagcgc
     1441 ggccatgtcg aacggctcgt cgctccagtc gccgggcgcg gccgcgggcg ccggcggcgg
     1501 cgctccgccc cctccgccgc cgccgcccga cggtggcgcc ggctcggcgg gcggcggcag
     1561 gaaggcgttg gcgtccagcc ggtcgatggt cagcgcggca ttgaacaccg ggaccgcgcc
     1621 ggcgctgtcg aagcggccat cggcgcgcgc ccggaacgcc gcaccctcga cggtcgcctc
     1681 ggacagggtc accaccggcc cgctggcgcc gagcgtcgcc ttgaccgcga gcgcgccggg
     1741 gtccggctgg ccggcgggca gcggctggcc gagccaagcc agcagccggc cgaccgaggc
     1801 ggtctcgacc gcgaggccgc cgtcgaggcc cggggtcggg cggctctgca ccgcgccctt
     1861 atacgacgcc gtgaccagcg ccgagtcgac cgaaaggtcg accccgaagc ggtcctcgcc
     1921 gaggaccttg ggcagcggat cgacggcggc ggcaagcgcc acctgctcgc ccttgtagac
     1981 cgcgctcgcg tcgatcgccg ggccggcgtc gatgccggtg agcgaggcct tgagcgacag
     2041 ctcggacagg ccggcgaacg cctcgtcgct ggtctcgatg ccggcgaggg cgacgtcgac
     2101 attggcggca agcgactcgg cgagcgcgcg cgcattcgcg ccgcgtccgg tcgcgctgac
     2161 gcggcccgag gcgacgccgg cgaccggcag cgcggcgcct gcggcctcgg cgaggcgcgc
     2221 gacggcgacc ttgtcgaccg tcacctcggc gtcgaccgac agctcgtcgc ccgagccgtc
     2281 ggcggcgacc tgcgccgtca cctcgccgcc gtacaacccg agctgctcga tgcgcatctg
     2341 gtggacgccg cccgccatcg ccccggacag caccaccggc cccacctcgt agcccatcgc
     2401 ccgcagcgcg ccgatgcgca ccgacacctg tccttccatg gtgcgcatgt gggccaggtc
     2461 gaacggccga tccgggatcg cggcaaacag gtccgcctgg gcggcgggcg ccggcgccgc
     2521 ggccgcggcc ggctctgccg cggcctgctc tgccacggcc tgttccaccg gcgccggcgg
     2581 cggcagatag cggtcgatgt cgagcgagtc tgcctcgagc gtgaagtcga ccttttgcac
     2641 cgtcgcaccg cgctcgaagc tgccgtgggc gcgggcgttc agcccctcgc ccgcgatcga
     2701 cgcctcggac agcaccacct tggcgccgtc ggcggcgacc gccgcgcgca ccgacagcgg
     2761 cccgggatcg ggctggccct cgggcagcgg ccggccgagc cagcccgcga gcttgccgac
     2821 cgagccggcg tcgagcgcga acgcgccgtc gagcgcaggc accgggcgct gctgcaccgc
     2881 gcccttgtag gacgccgtga ccagcgccga gtcgaccgaa agatcgaggt cgaagcggtc
     2941 ctcgccgagc accttcggca gcggatcgct ggtggcggcg agcctcaccc gctggccgtt
     3001 gtagaccagc tccacgttga ccgccggcgc cgcgtcgatc cccggcagat cgaccgcgag
     3061 cgcgagctcg gagaccgcgc gcaaggcctc gtccgtggcc tcgaccccgc cgaggtcgag
     3121 ctcgacccgc ccggataggt tctcggccag cgcgcgcggg ctggcgccgc ggctcttggc
     3181 gaccagcgcg cccgaggcaa tgccgaccat cggcaccgcc gcccccgcgg cctcggcgag
     3241 ctcgccgacc gcgaccttgt cgataccgaa gtcggcgtcg agcgtgatct cgcccgcggt
     3301 ctcggcgggc gcaaccgcca gccggccttc gaccgtgccg ccgtagaggc cgagctcggc
     3361 gatctcgaag ctggcgctgc cgcctgccgc ctttagagac accgacagcg gcccggcgcg
     3421 atagcccatg gcgctgagcg cgccgagctt gatcgcgata tcggcgtcga tggcatccag
     3481 cgcggtgagg tcgagcggcg cgtcgggcac ggcggcgaac atgtcggcct tggccgccgg
     3541 cgcgccctgg tccccggcag gcgcggcggg cggcggcgcc ggctccgccg gcaacgcctg
     3601 tgcgccggcg ggccgcggcg gcaggtagcg gtcgatatcg agcgactcgc cctcgagccg
     3661 gaacgcgacc ttggtcacct cgccgccggc atcgatcgag ccgaaggcgg tggcatcgag
     3721 cccctcgccc cggatcgtcg cctcggtcag cgccaccgac tggccctccg cgaccagctt
     3781 cgccgccagc gcgagcgggc cggggtcggg ctggtccgcc ggcagcggcc ggccgagcca
     3841 ggacaagagc gcgccgaccg aggccacgtc gagcgcgagc gcgccgtcga gcccgggcaa
     3901 gggccgggtc tgcaccgcgc cgtcgtagct cgcggtgacc cgcttcgagt cgagcgcggc
     3961 ggcgagcgtg aaccggttgc cggccagcgc atcgccgaaa ttggtgaccg tgaccttggc
     4021 cgagaccggc tcgtcgttga gggtgagcgc gccggcgaag gtgagcggcg cgtccggcgc
     4081 cggcgcggcg agggacgcgc tgat
//
LOCUS       DASWHU010000235         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3431370, whole genome shotgun sequence.
ACCESSION   DASWHU010000235 DASWHU010000000
VERSION     DASWHU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3431370"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..868)
                     /gene="dapB"
                     /locus_tag="VGB88_08615"
     CDS             complement(56..868)
                     /gene="dapB"
                     /locus_tag="VGB88_08615"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9647546.1"
                     /translation="MADLDIGVVGAAGRMGTALIRRIAGTEGCRLAAAIESPGHPAAG
                     RDAGAEAGIAALGIAIGEDARALFEAADVVIEFSSPAATAAHAALAGATGTAHVIGTT
                     GLDAAQAAAVRKAARSAAVVWAPNMSIGVNLLLALTEQVAAVLDDAYDIEIVEMHHRH
                     KVDAPSGTALGLGRAAAKGRGVDLEKVSARARDGVTGARRTGDIGFAVLRGGDVVGDH
                     AVVFAGEGERLELGHRASSREVFAAGAVRAALWTRGRAPGLYGMREVLGLAG"
     gene            complement(903..1541)
                     /locus_tag="VGB88_08620"
     CDS             complement(903..1541)
                     /locus_tag="VGB88_08620"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9647547.1"
                     /translation="MDRINVGILVFDDVEVLDFSGPFEVFSRTRTVPGIASRRSEDTA
                     PFRVFTVARTRDPVTAVGGLEVMPGYGFAEAPAIDVLLVPGGFGTRALLEDAATLVWV
                     KRVAAGARLTTSVCTGALVLARAGLLAGRRATTHWGATELLESLDAGVTVERDVRYVD
                     DGIITSAGVAAGIDMAFHVVERLCGKAVADETARYIDYPRAAPSLAGAATGG"
     gene            complement(1550..>2016)
                     /locus_tag="VGB88_08625"
     CDS             complement(1550..>2016)
                     /locus_tag="VGB88_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647548.1"
                     /translation="ATSEWMRWRYSQHLFAGYGETCLALDDPAGAKAWADQCLELATR
                     TDSKKYLVRGWRLKAGIEVARLSWEDAEEALLRALAIAERIGNPPQLWKTRLALSRLY
                     RDTRRPDRARVAAVAARKVLDGIGAGLKTPSLRDSFAGSFVIRSAYELAAVS"
BASE COUNT          282 a          776 c          711 g          247 t
ORIGIN      
        1 tcctccgcag gcctgtcgcg aggccggacg cgggccttca ggttccgggc acggcctaac
       61 cggcgaggcc gagcacctcg cgcatgccgt agagcccggg cgcgcgcccc ctggtccaca
      121 gcgccgcgcg caccgcgccg gcggcgaaca cctcgcgcga ggacgcccgg tggccgagct
      181 cgaggcgctc gccctcgccc gcgaacacca ccgcgtggtc gccgaccacg tcgccgccgc
      241 gcaacaccgc gaagccgatg tcgccggtcc ggcgcgcgcc cgtcacgccg tcgcgcgcgc
      301 gcgccgagac tttctccaga tcgaccccgc gccccttggc cgcggcgcgc ccgagcccga
      361 gcgcggtgcc cgacggcgca tcgaccttgt ggcggtggtg catctcgacg atctcgatgt
      421 cgtaggcgtc gtcgagcacc gccgccacct gctcggtgag cgcgagcaac aggttgacgc
      481 cgatcgacat gttgggcgcc cacaccaccg ccgcgctgcg ggcggccttg cgcaccgccg
      541 ccgcctgcgc cgcatccagg ccggtggtgc cgatgacgtg cgcggtgccg gtggcgccgg
      601 cgagcgccgc gtgggccgcg gtcgcggcgg gcgaggagaa ctcgatcacc acgtccgcgg
      661 cctcgaacag cgcgcgcgcg tcctcgccga tggcgatgcc gagcgcggcg atcccggcct
      721 cggcgccggc gtcgcgcccc gcggccgggt gccccggact ctcgatcgcc gccgccagcc
      781 ggcagccttc ggtgccggcg atgcggcgga tcagcgccgt gcccatgcgc cccgccgcgc
      841 ccaccacccc gatgtcgaga tcggccatcg cttgctccag cccgcctgcg gtcgcgaacg
      901 gtctagccgc cggtcgcagc cccggcaagg gaaggggcgg ccctgggata gtcgatgtag
      961 cgcgcggtct cgtcggcgac cgccttgccg cacagtcgct cgaccacgtg gaacgccatg
     1021 tcgatgccgg cggcgacccc ggccgaggta atgatgccgt cgtcgacata gcggacatcg
     1081 cgctcgaccg tcaccccggc atcgagcgat tccaaaagct cggtcgcgcc ccaatgggtg
     1141 gtcgcccgcc ggcccgcgag caggccggcg cgtgccagca ccagcgcgcc ggtgcacacc
     1201 gaggtggtca gccgggcacc cgccgcgacc cgcttgaccc agacgagggt cgcggcatcc
     1261 tcgagcagcg cgcgcgtgcc gaagccgccg ggcacgagca ggacatcgat agccggcgct
     1321 tcggcgaacc cgtagccggg catgacctcg agcccgccga ccgcggtcac cggatcgcgc
     1381 gtcctggcga ccgtgaacac gcggaacggc gcggtgtctt cggagcggcg cgaagcgatg
     1441 cccggcaccg tgcgggtgcg cgagaacacc tcgaacgggc cgctgaaatc gagcacctcg
     1501 acgtcatcga acaccaggat gccgacattg atcctgtcca tggcgcgagt cagctcaccg
     1561 ccgccagctc ataggcgctt cggatgacga acgagcccgc gaaagagtct cgcaaggacg
     1621 gcgtcttcaa gccggcgccg atgccgtcga gcaccttgcg tgccgcaacc gccgcgacgc
     1681 gcgcgcggtc gggccggcgg gtgtcccgat acagccggct caaggcgagc cgggtcttcc
     1741 acagctgggg cgggttgccg atccgctcgg caatggcgag tgcgcgcagc aaggcctcct
     1801 cggcgtcctc ccagctcagc cgggcaacct cgatgccggc cttgagccgc cagccgcgca
     1861 ccaggtactt cttcgagtcg gtgcgggtcg cgagctcgag gcactgatcg gcccacgcct
     1921 tggcgccggc ggggtcgtcg agggcgaggc aggtctcgcc atagcccgcg aacaggtgct
     1981 gcgagtagcg ccagcgcatc cactcgctgg tcgccg
//
LOCUS       DASWHU010000236         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2713026, whole genome shotgun sequence.
ACCESSION   DASWHU010000236 DASWHU010000000
VERSION     DASWHU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713026"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1306
                     /gene="folP"
                     /locus_tag="VGB88_08630"
     CDS             167..1306
                     /gene="folP"
                     /locus_tag="VGB88_08630"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9647549.1"
                     /translation="MERLTVTRSPAPPRGFGDSERAYLLPTALLRGESARRALAGGAA
                     LPLAGGPVAFAACEVVVRAPGAALASWLEVPALKRWRDSLADPAPLDQLLERLCAPRP
                     PFAAIPLDRPRLMGVVNVTPDSFSDGGRFLDAERAIARGRALADAGADIVDVGGESTR
                     PGAAPIAPEEEQARAVPVVRALAEAGLTVSIDTRNPATMAAAAAAGAAIINDVSALAH
                     HPDSAARAAATGCAVVLMHSAGDPRSMQDRPAYDHVALDVYDALAARVAAAEAAGIAR
                     ARLAVDPGFGFGKTPAHNLRLVDWLALLHGLGCALVVGASRKSTIARIAGGMAEDAGG
                     RLAGSLALATAAWDRGAQILRVHDVAETAQALALWRALDDAGSPP"
     gene            1384..2730
                     /gene="glmM"
                     /locus_tag="VGB88_08635"
     CDS             1384..2730
                     /gene="glmM"
                     /locus_tag="VGB88_08635"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9647550.1"
                     /translation="MPRTLFGTDGIRGRANLEPMTAETALKVGMAAGKVFSRGAHRHH
                     VVIGKDTRLSGYMIEPALTAGFTSMGMNVILVGPMPTPAIGMLTRSLRADLGVMISAS
                     HNPYWDNGIKLFGPDGFKLSDAVEREIEAAIDNGVADGLAAPEYVGRARRLDDAQGRY
                     IEFAKATFPKGMTLNGIKAVLDCANGAAYRVAPTVLWELGAGVVPIGIDPDGFNINQG
                     CGSTAPEALCREVVAQGADIGIALDGDADRVVMCDEQGALIDGDQLMALVAEHFHRHG
                     RLAGGGVVATQMSNLGLERYLDSLGLHLVRTPVGDRYVVEHMRAHGYNVGGEQSGHLI
                     LTDYATTGDGLVAALQALAVLVEQDQPASAAARRFEALPQHLKSIACNGAGVLECGEV
                     MTAIGDARGLLGGAGRLLVRMSGTEPKVRVMAEGDDEAAVLRAIEAVVQAIERQAP"
     gene            2727..3551
                     /gene="thiD"
                     /locus_tag="VGB88_08640"
     CDS             2727..3551
                     /gene="thiD"
                     /locus_tag="VGB88_08640"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX9647551.1"
                     /translation="MKGRVLVIAGSDSGGGAGIQADIKTVTALGGYAACAITALTAQN
                     TVAVTGIHEVPAEFVAEQMVVVLDDIGADALKTGMLHRVDVIEAVARVIAERARDVPL
                     VVDPVMVAKSGTPLITEDAQGALAARLLPLARLVTPNIPEAARLAGMDIAAEADMAEA
                     ARRILALGPKAVLVKGGHLEGETLVDLLVDGEGARRFTGPRIASRNTHGTGCTLASGI
                     AIGLAQGMALADAVARARAYVTAAIRSAPGLGAGHGPLDHAHPLAACTGARKGDVA"
BASE COUNT          480 a         1378 c         1343 g          480 t
ORIGIN      
        1 gcggcgctgc cgctgctgcg gaggacgggc cgctcgctcc tcaggacgag ggagttttga
       61 aaatcaagca attacctcac cctgaggagc gcccgacggg cgcgtctcga agggtcaggc
      121 aaaagattca caggctctga gtccgtcgcg cccgcgcgcg ctgcgcatgg aacggctgac
      181 ggtcacccga agccccgccc cgccgcgggg cttcggcgat tcggagcgcg cctatctgct
      241 gccgaccgcc ttgctgcgcg gcgagagcgc gcggcgcgcg ctcgccggcg gcgcggcgtt
      301 gccgcttgct ggcgggccgg tggcgttcgc cgcctgcgag gtggtggtgc gcgcgcccgg
      361 cgcggcgctg gcgagctggc tcgaggtccc ggcgctgaag cgctggcgcg actcgcttgc
      421 cgacccggcg cccctcgacc aattgctcga gcgcctgtgc gcgccccggc cgccgttcgc
      481 ggccatcccc ctcgaccgtc cgcggctgat gggcgtggtc aacgtcaccc ccgacagctt
      541 ctcggacggc ggccgcttcc tcgatgccga gcgcgccatc gcccgcgggc gtgcgctcgc
      601 ggatgcgggt gccgacatcg tcgatgtcgg cggcgagtcg acccgccccg gcgccgcgcc
      661 gatcgcgccc gaggaagagc aggcgcgcgc cgtgccggtg gtgcgcgccc tcgccgaggc
      721 cggcctgacg gtctcgatcg acacccgcaa cccggcgacg atggccgccg cggccgcggc
      781 cggcgccgcg atcatcaacg acgtgagcgc cctcgcccac caccccgaca gtgccgcgcg
      841 cgccgccgcc accggctgcg cggtggtgct gatgcacagc gccggcgacc cgcgcagcat
      901 gcaggaccgc cccgcctacg accacgtcgc gctcgacgtc tatgacgcgc tcgccgcgcg
      961 cgtcgccgcc gccgaggccg cgggcattgc gcgtgcaagg ctcgccgtcg accccggctt
     1021 cggcttcggc aagacgccgg cgcacaacct gcgcctggtc gactggctgg cgctgctgca
     1081 cgggctgggc tgcgcgctgg tcgtcggcgc ctcgcgcaag agcaccatcg cccgcatcgc
     1141 cggcggcatg gccgaggacg ccggcggccg ccttgccggc tcgcttgcgc tggccacggc
     1201 ggcgtgggac cgtggcgccc agatcctgcg cgtccacgac gtcgccgaga ccgcgcaggc
     1261 gctggcgctg tggcgcgcgc tcgacgacgc cggctcgccg ccctgatccg gcttgtgcgc
     1321 cggcggcggc ggcgctaagt gctgtcgccg gcgctgcacg ccggcgagga gggatcgagt
     1381 cgcatgcccc gcaccctgtt cggcaccgac ggcatccgcg gccgtgccaa tctcgagccg
     1441 atgaccgccg agaccgcgct caaggtgggc atggccgcgg gcaaggtgtt cagccgcggc
     1501 gcgcaccgcc accacgtggt gatcggcaag gacacccgcc tgtcgggcta catgatcgag
     1561 ccggcgctga ccgccggctt cacctcgatg ggcatgaacg tgatcctggt cgggccgatg
     1621 ccgacgccgg caatcggcat gctcacccgc tccttgcgcg ccgacctcgg ggtgatgatc
     1681 tcggcctcgc acaaccccta ctgggacaac ggcatcaagt tgttcggccc cgacggcttc
     1741 aagctctcgg atgcggtcga gcgcgagatc gaggccgcga tcgacaacgg ggtggccgac
     1801 ggcctggcgg cgcccgagta tgtcggccgc gcgcgccggc tcgacgacgc ccagggccgc
     1861 tatatcgagt tcgccaaggc gacctttccg aaaggcatga ccttgaacgg catcaaggcg
     1921 gtgctcgatt gcgccaacgg cgccgcctat cgggtcgcgc cgacggtgct gtgggaactc
     1981 ggcgccgggg tggtgccgat cgggatcgac cccgacggct tcaacatcaa ccagggctgc
     2041 ggctcgaccg cgcctgaggc gctgtgccgc gaggtcgtgg cccagggcgc cgacatcggc
     2101 atcgcgctcg acggcgacgc cgaccgggtg gtgatgtgcg acgagcaggg cgcgctgatc
     2161 gacggcgacc agctgatggc gctggtcgcc gagcacttcc accgccacgg gcggctcgcc
     2221 ggcggcggcg tggtcgcgac ccagatgtca aacctcggcc tcgagcgcta cctcgactcg
     2281 ctcggcctgc acctggtgcg caccccggtc ggcgaccgct acgtggtcga gcacatgcgc
     2341 gcccacggct acaacgtcgg cggcgagcag tccgggcacc tgatcctgac cgactatgcc
     2401 accaccggcg acggcctggt tgcggcgctg caggcgctcg ccgtcctggt cgagcaggac
     2461 cagccggcga gcgccgccgc gcgccggttc gaggccctgc cccagcacct gaagagcatc
     2521 gcctgcaacg gcgcgggcgt gctcgaatgc ggcgaggtga tgaccgcgat cggcgatgcg
     2581 cggggcttgc tcggcggcgc cggccgcttg ctggtgcgca tgtcgggcac cgagcccaag
     2641 gtgcgggtga tggccgaggg cgacgacgag gcggcggtgc tgcgcgccat cgaggcggtg
     2701 gtccaggcga tcgagcgcca ggcgccatga aggggcgcgt gctggtcatc gccggctcgg
     2761 attcgggcgg cggcgccggc atccaggcgg acatcaagac cgtcaccgcg ctcggcggct
     2821 atgcggcgtg cgcgatcacc gcgctcaccg cccagaacac cgtcgccgtc accggcatcc
     2881 acgaggtgcc ggccgagttc gttgccgagc aaatggttgt ggtgctcgac gacatcggcg
     2941 cggatgcgct caagaccggc atgctccacc gcgtcgacgt gatcgaggcg gtggcgcgcg
     3001 tgatcgccga gcgcgcgcgc gacgtgccgc tggtggtcga cccggtgatg gtggcgaaaa
     3061 gcggcacgcc cctgatcacc gaggacgcgc aaggcgccct cgccgctcgg ctgctgccgc
     3121 tcgcccgcct ggtcaccccg aacatccccg aggcggcgcg cctcgccggc atggacatcg
     3181 cggccgaagc cgacatggcg gaagccgcgc gccgcatcct tgcgctcggg ccgaaggcgg
     3241 tcctggtcaa gggcggccat ctggaaggcg agaccttggt cgacctgctg gtcgacggcg
     3301 agggcgcgcg ccggttcacc ggaccgcgga tcgccagccg caacacccac ggcaccggct
     3361 gcacgctcgc ctcaggaatc gccatcggcc tcgcccaagg gatggcgctc gccgacgcgg
     3421 tggcgcgcgc gcgcgcctac gtcaccgcgg cgatccgcag cgcccccggc ctcggcgccg
     3481 gccacggccc cctcgaccac gcgcatccgc tcgccgcctg caccggcgca cgcaagggcg
     3541 atgtcgcgtg agtcaagcgg cgggcgagtc gcgctgggtt agctcgaatc cggccacgcg
     3601 cgcgccttgg cgggcggcct gctcggcgcc gaccagctcg cgcggctcgc tcgtacgcgc
     3661 cgccaccgtg ccgcggatcg g
//
LOCUS       DASWHU010000237         6326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1989387, whole genome shotgun sequence.
ACCESSION   DASWHU010000237 DASWHU010000000
VERSION     DASWHU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989387"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGB88_08645"
     CDS             complement(<1..344)
                     /locus_tag="VGB88_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008280773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9647552.1"
                     /translation="MQAIRYEAPTTIEAAVALLAGADGAAKVLAGGTDLLAQMRSGMV
                     RPALIVDVKGIPELTAILADRGGGFRIGAAVSGAVLGEREDVRAAWPGVVEAHELIGS
                     TQIQGRASLGGNL"
     gene            complement(660..1016)
                     /locus_tag="VGB88_08650"
     CDS             complement(660..1016)
                     /locus_tag="VGB88_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647553.1"
                     /translation="MRLTVRDLAHTRAGDKGHAVNVSVTAFDDAGYQRLERELTEARV
                     MQAFAAVADGPVRRYRLPGLRALNFVIDHIRGGGVTATLAQDIHGKSLSSLMLTIDLP
                     GNEPQEAGQAALRSEA"
     gene            complement(1013..2356)
                     /locus_tag="VGB88_08655"
     CDS             complement(1013..2356)
                     /locus_tag="VGB88_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX9647554.1"
                     /translation="MRRTVRIGSGAGYAGDRWEPAVELAEKGEVDYIAFECLAERTIA
                     RETLAMHHDPARGYNPRLTERMAAVLPPCARNRVRVVTNMGAANPLGAGRETCRVAAE
                     AGLKDLRCAVLLGDDVSDTVRAMPGLALLETGQPLESILPRMASANAYLGADAIRDAL
                     ATGAEVVMTGRVADPSLFVAPALFEFGWSYDDYPRLAQATVAGHLLECAGQVTGGYFA
                     DPGPKDVPALARLGFPFADVSADGEVAIGKVAGSGGRIDAMTCTEQLLYEMGDPAAYI
                     TPDCVLDVTALGFVEERADRVRVAGARARPRTPTYKVSVGYFDGYMGEGQISYGGPNA
                     VARARLAGEVVAERLRLRGLAYDELRCELIGLDSLHGPGAGRPEPYEVRLRVAGRTAS
                     RAAAEELGWEVETLLTNGPQGGAGHFGQVREIYAVQSVLLAREAVTTRIETVAAR"
     gene            complement(2370..3572)
                     /locus_tag="VGB88_08660"
     CDS             complement(2370..3572)
                     /locus_tag="VGB88_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9647555.1"
                     /translation="MDFDYSDKVKALQERVSAFMDAQVYPNEAKIAAQIAAARWQPPP
                     LMEEIKAAARKAGLWNLFLPESEYGAGLTNLEYAPLCEIMGRSPFAPEAFNCSAPDTG
                     NMEVLARYGTEAQKTQWLDPLLDGRIRSAFAMTEPQVASSDATNIEASIRRDGDGYVI
                     NGRKWWTSGAPDPRCKILIFMGKTDAANPDKHKQQSMILVPLDTPGIKVERILPVFGY
                     DDSPHGHAEVSFTNVRVPASNMLLGEGRGFEIAQGRLGPGRIHHCMRLIGLAERALER
                     MCKRVKGRIAFGKPVAEQTVTQERIAEARIMIDQCRLLVLNAAYKMDTVGNKEARKEI
                     AMIKVAAPNMACRVIDWAIQAHGGGGVTEDFGLAAAYANARTLRLADGPDEVHRNQIA
                     RLELRRYN"
     gene            3751..5334
                     /gene="otsB"
                     /locus_tag="VGB88_08665"
     CDS             3751..5334
                     /gene="otsB"
                     /locus_tag="VGB88_08665"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9647556.1"
                     /translation="MTQRSRAAVRAPRPAPRPARRERRIEALLVDLDGVVTRTAKVHA
                     QAWKRLFDAFLDRRGGRWQPFDEVADYRVHVDGKPRYDGVRDFLAARGITLAEGRPAD
                     APGWDTVCALGNMKDGYFQEALAAGGVEVFEATVARLRAAKADGLRLALVSSSRNAGA
                     VLAACGLDDLFEVRVDGIDIDREGLPGKPKPDMFLRAAARLGVEPARAGVIEDAIAGI
                     AAARAGNFALSIGIDRGGQAEALRAAGADLVVADLAHVDFDAAASARPPSALDSFDAI
                     AARLAGKRAAVFLDYDGTLTPIVARPELALLADAARAAVDRLARLCPVAVISGRARAD
                     VRRLVGLESVIYAGSHGFDIEAPGGRALGHVVGREYVAAIAAAADELRARLAAIEGVI
                     VEDKTYALAIHYRLVAKSDVAAVESAVDRAAAAHPELRKTLGKKVFELRPALAWDKGK
                     AVLWLLEALNLGQDVVPVYLGDDVTDYDAFDALKGRGIGILVSDGPPPRPVDYVLRDP
                     HEVRVFLDRLAELLAAARR"
     gene            5331..>6326
                     /locus_tag="VGB88_08670"
     CDS             5331..>6326
                     /locus_tag="VGB88_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEX9647557.1"
                     /translation="MTDWLLAYDRLEPARERVREALCALGNGRFCTRGAAAEAAADDV
                     HYPGCYAAGCYNRLASEVAGRTIEHEDLVNLPNWLALGWRIGDDDWFDPGRVDILSYR
                     AELDLRRGVFTRELLCRDHAGRRTRVSTRRMVSMAEPHLAGIEMTLAAENWSGPVTVR
                     WALDGRVTNAGVARYRALAGRHLMPVAAGQAGDGIIHLTVETNQSRIRIAEAARARIL
                     VAGVEAEVDTALHTARGYVACDATLALAQGVPVTVEKLVALYTSRDPAISECALAARR
                     AVGRAPDLGRLLGAHARTWRYLWEQFGIDVDVDGSPSNRMVATLRLHLFHLLQTAS"
BASE COUNT          819 a         2317 c         2281 g          909 t
ORIGIN      
        1 aggttgccgc cgagcgaggc acgaccctgg atctgggtcg agccgatcag ctcgtgcgcc
       61 tcgaccacgc ccggccaggc cgcgcgcacg tcctcgcgct cgccgagcac ggcgccgctc
      121 accgccgcgc cgatgcggaa gccgccgccg cggtcggcga ggatcgccgt cagctcgggg
      181 atgcccttga cgtcgacgat cagcgccggg cggaccattc cggagcgcat ctgcgccagc
      241 aggtcggtgc cgccggcgag caccttggcc gcaccgtcgg cgccggcgag cagcgccacc
      301 gccgcctcga tcgtggtcgg agcctcgtat cgtatcgcct gcatcgtccc tctcgtcgat
      361 cgcgcgcacc acggcgccgc catggccgtt accgcgactg ccgtgccgtt ttagcccccg
      421 ggtatcggcc cccacaatag ccgggcggag gccaaagcgg aacctccaag cgcgccgccc
      481 ggccagcccc ttgcggacca gctaaagcgt tctccgccgg ccttgactca gtcccccctc
      541 acccagcttc gggtaaggcg cggctggcgc cgcgccaacc cttcgcaacc ctctcccccc
      601 gccacgcgga gggagagggc agggtgaggg gggctggtcc gaaccgagcg caccatgcac
      661 taggcctcac tccgaagtgc agcctgcccg gcctcttggg gctcgttgcc gggcaggtcg
      721 atggtcagca tcagcgagct caggctcttg ccgtggatgt cctgggcgag cgtcgcggtg
      781 acgccgccgc cgcggatgtg gtcgatgacg aagttgagcg cgcgcaggcc cggcagccgg
      841 tagcggcgga ccgggccgtc ggcgaccgcg gcgaaggcct gcatcacccg cgcctcggtc
      901 agctcgcgct cgagccgctg gtagccggcg tcgtcgaacg ccgtgaccga gacgttgacc
      961 gcatggccct tgtcgccggc gcgggtgtgg gcgagatcgc ggaccgtcag cctcatcgcg
     1021 ccgcgaccgt ctcgatgcgg gtcgtcaccg cctcgcgcgc gagcagcacc gactggacgg
     1081 cgtagatctc cctgacctgg ccgaaatggc cggcgccgcc ctgggggccg ttggtgagca
     1141 gggtctcgac ctcccagccg agctcctcgg cggcggcgcg cgaggcggtg cgcccggcga
     1201 cgcgcaggcg cacctcgtag ggctcgggcc ggccggcgcc gggcccgtgc aggctgtcga
     1261 gcccgatcag ctcgcagcgc agctcgtcgt aggcgaggcc gcgcagccgc aggcgctccg
     1321 cgaccacctc gccggccagg cgcgcgcgcg ccaccgcgtt cggcccgcca taggagatct
     1381 ggccctcgcc catgtaaccg tcgaagtagc cgacgctgac cttgtaggtg ggggtgcgcg
     1441 ggcgggcgcg cgcgccggcg acgcgcacgc ggtccgcccg ctcctcgacg aagccgagag
     1501 cggtcacgtc gagcacgcag tcgggcgtga tataggccgc cggatctccc atctcgtaga
     1561 gcagctgctc ggtgcaggtc atcgcgtcga tgcgcccgcc gctgcccgcg accttgccga
     1621 tcgccacctc gccgtcggcg ctgacgtcgg cgaacgggaa gccgaggcgg gcgagcgccg
     1681 gcacgtcctt cggccccggg tcggcgaagt agccgccggt gacctggccc gcgcactcga
     1741 gcagatgccc ggcgacggtc gcctgggcga gccgcggata gtcgtcgtag gaccagccga
     1801 actcgaacag cgccggggcg acgaacagcg acgggtcggc gacccgcccg gtcatcacca
     1861 cctcggcgcc ggtggcgagc gcgtcgcgga tcgcatcggc gccgagatag gcgttggccg
     1921 aggccatccg gggcaggatc gattcgagcg gctggccggt ctcgagcagc gcgagcccgg
     1981 gcatcgcccg caccgtgtcg ctgacgtcgt cgccgagcag caccgcgcat ctcagatcct
     2041 tgaggccggc ctcggcggcg acccggcagg tctcgcgccc ggcgccgagc gggttggccg
     2101 cgcccatgtt ggtgaccacg cgcacgcggt tgcgcgcgca gggcggcagc accgcggcca
     2161 tgcgctcggt cagcctcggg ttgtagccgc gcgccgggtc gtggtgcatg gccaaggtct
     2221 cgcgcgcgat ggtccgctcg gccaggcact cgaaggcgat gtagtcgacc tcgcccttct
     2281 cggcgagctc gaccgcgggc tcccagcggt cgccggcata gccggcgccg gagccgatgc
     2341 ggaccgtgcg tctcaccgct gcgcggccct cagttgtagc gcctgagctc gaggcgggcg
     2401 atctggttgc ggtggacctc gtccgggccg tcggcgagcc tgagggtgcg cgcgttggcg
     2461 taggccgcgg ccaggccgaa gtcctcggtc acgccgccgc cgccgtgggc ctggatcgcc
     2521 cagtcgatca cccggcaggc catgttgggc gcggcgacct tgatcatcgc gatctccttg
     2581 cgcgcctcct tgttgccgac ggtgtccatc ttgtaggcgg cgttgagcac cagcaggcgg
     2641 cactggtcga tcatgatccg cgcctcggcg atgcgctcct gggtcacggt ctgctcggcg
     2701 accggcttgc cgaaggcgat gcgccccttg acccgcttgc acatgcgctc gagcgcgcgc
     2761 tcggcgagcc cgatcaggcg catgcagtgg tggatgcgcc ccgggccgag ccggccttgc
     2821 gcgatctcga agccgcggcc ctcgccgagc agcatgttcg acgccggcac gcgcacgttg
     2881 gtgaagctga cctcggcgtg gccgtgcggg gagtcgtcgt agccgaacac gggcaggatg
     2941 cgctcgacct tgatccccgg agtgtcgagc ggcaccagga tcatcgactg ctgcttgtgc
     3001 ttgtcggggt tcgcagcgtc ggtcttgccc atgaagatca ggatcttgca gcgcgggtcg
     3061 ggcgcgcccg aggtccacca cttgcggccg ttgatcacgt agccgtcgcc gtcgcgcctg
     3121 atcgaggcct cgatgttggt cgcgtccgag gacgccacct gcggctcggt catcgcgaat
     3181 gccgagcgga tgcgaccgtc cagcagcggg tccagccact gcgtcttctg cgcctcggtg
     3241 ccatagcggg cgagcacctc catgttgccg gtatcggggg cggagcagtt gaaggcctcg
     3301 ggcgcgaacg gcgagcggcc catgatctcg cacagcggcg cgtattcgag gttggtgagc
     3361 ccggcgccgt actcggattc gggcaggaac aggttccaca gtccggcctt gcgcgccgcg
     3421 gccttgatct cctccatcag cggcggcggc tgccagcgcg cggcggcgat ctgggccgcg
     3481 atcttcgcct cgttgggata gacctgcgcg tccatgaagg ctgagacgcg ctcctgcaac
     3541 gccttgacct tgtccgaata gtcgaaatcc attgccctgt ccccgactcc gcgtgcccgc
     3601 cggacctggc gcgccggctc gggcacggta gcagaacttt ccggcaaagg tgtagccgcg
     3661 cggctggcgg cctcgcataa agacgcaaaa gggttacatt tcagctggtg caacggggcc
     3721 catcggtcga gccatgccgc gagccaggac atgacccagc gctcccgcgc cgccgtgcgc
     3781 gcgccgcgcc ctgcgccccg ccccgcccgg cgggagcgtc gcatcgaggc cctgctggtc
     3841 gacctcgacg gcgtggtgac gcggaccgca aaggtccatg cccaggcctg gaagcggctg
     3901 ttcgacgcgt ttctcgaccg ccgcggcggc cgctggcagc cgttcgacga ggtcgccgac
     3961 tatcgcgtcc atgtcgacgg caagccccgt tacgacgggg tgcgcgactt cttggccgcg
     4021 cgcggcatca cgctcgccga gggccggcct gcggacgcgc ccggctggga cacggtgtgc
     4081 gcgctcggca acatgaagga cggctatttc caagaggcgc tggccgcggg cggagttgag
     4141 gtgttcgagg cgacggtggc gcggctgcgc gcggcgaagg cggacgggct gcgcctcgcg
     4201 ctggtgtcgt cgagccgcaa cgcaggcgcg gtgctcgcgg cgtgcggcct cgacgacctg
     4261 ttcgaggtcc gcgtcgacgg catcgacatc gaccgcgagg gcctgcccgg caagcccaag
     4321 cccgacatgt tcctgcgcgc cgccgcccgg ctcggcgtcg agccggcgcg cgccggcgtg
     4381 atcgaggacg cgatcgccgg catcgcggcc gcgcgtgccg gcaacttcgc gctctccatc
     4441 ggcatcgacc gcggcgggca ggccgaggcg ttgcgcgcgg ccggagccga cttggtggtc
     4501 gccgatctcg cccacgtcga tttcgacgcc gcggcatcgg cgcgcccccc ttccgcgctc
     4561 gatagcttcg acgccatcgc cgcccgcctc gccggcaagc gggcggcggt attcctcgac
     4621 tacgacggca ccctgacacc gatcgttgcc cggcccgagc tcgcgctgct cgccgacgcc
     4681 gcccgggcgg cggtcgatcg cctggcccgg ctgtgcccgg tggcggtgat cagcggccgg
     4741 gcgcgcgccg acgtccggcg cctggtcggc ctcgagtcgg tgatctacgc cggcagccac
     4801 ggtttcgaca tcgaggcgcc cggcgggcgc gccctcggcc acgtggtggg gcgcgagtac
     4861 gtggccgcga tcgccgccgc cgccgatgag ctgcgcgcgc gcctggccgc gatcgagggc
     4921 gtgatcgtcg aggacaagac ctatgcgctg gcgatccatt accgcctggt tgcgaaaagc
     4981 gatgtcgcgg cggtcgagag cgccgtcgac cgcgccgccg ccgcgcatcc ggagctgcgc
     5041 aagacgctcg gcaagaaggt gttcgagctg cgtccggcgc tcgcctggga caagggcaag
     5101 gcggtgctgt ggctgctcga ggcgctcaat ctgggacagg acgtggtgcc ggtctatctg
     5161 ggcgacgacg tgaccgacta cgacgcgttc gacgcgctca aggggcgcgg catcggcatc
     5221 ctggtgtcgg atggcccgcc gccgcggccg gtcgattacg tcctgcgcga tccacacgag
     5281 gtgcgcgtct tcctcgaccg cctggccgag ctgctggcgg cagcgcgccg atgaccgact
     5341 ggctgctcgc ctacgaccgc ttggagcccg cgcgcgagcg cgtccgcgag gccctgtgcg
     5401 cgctcggcaa cggccgcttc tgcacccgcg gcgcggccgc ggaagctgcc gccgacgacg
     5461 tccactaccc gggctgctac gcggccggct gctacaaccg cctggcgagc gaggtcgccg
     5521 gccgcaccat cgagcacgag gacctggtca acctgcccaa ctggctcgcg ctcggctggc
     5581 ggatcggcga cgacgactgg ttcgacccgg ggcgggtcga catcctgtcc taccgcgccg
     5641 agctcgacct ccggcgcggc gttttcacgc gcgagcttct gtgccgggac cacgccgggc
     5701 ggcgcacgcg cgtctcgacc cggcgcatgg tcagcatggc cgagccgcac ctcgccggca
     5761 tcgagatgac cctcgccgcc gagaactggt cgggcccggt caccgtgcgc tgggcgctcg
     5821 acgggcgcgt taccaacgcc ggcgtcgccc gctaccgggc gctcgcgggc cgccacctga
     5881 tgccggtcgc ggccgggcag gccggcgacg gcatcatcca cctgacggtc gagaccaacc
     5941 aatcgcgcat ccgcattgcc gaggcggcac gcgcgcgcat cctggtcgcc ggcgtcgagg
     6001 ccgaggtcga caccgcccta cacaccgcgc gcggctacgt cgcgtgcgac gccaccttgg
     6061 cgctcgccca gggcgtgccg gtcacggtcg agaagctggt cgcgctctac acctcgcgcg
     6121 atcccgccat ctccgagtgc gcgctcgccg cgcgccgggc ggtcggtcgc gcgcccgacc
     6181 tcggacgcct gctcggcgcg cacgcgcgca cctggcgcta tttgtgggag cagttcggaa
     6241 tcgatgtcga cgtcgacggc agcccgagca accggatggt ggcgacgctg aggctgcacc
     6301 tgttccatct gctccagacc gcgtcc
//
LOCUS       DASWHU010000238         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_117368, whole genome shotgun sequence.
ACCESSION   DASWHU010000238 DASWHU010000000
VERSION     DASWHU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117368"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VGB88_08675"
     CDS             complement(<1..740)
                     /locus_tag="VGB88_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX9647558.1"
                     /translation="MTIELVQRLSELEDRFDGLILDVWGVVMDGTAPYPGARDCLAEL
                     RRRGKRVVLLSNAPRRDSQVVARLAEIGIEAGLYDRIVSSGEATRQALEARTDPAVAG
                     FGRRYLYLGPERDRALLDGLDYAPSGMAEADFVLAIGIEDESDSVEKYHPLLKAAAAR
                     GLVLVCANPDRVVVRQSGERVLCAGALAEAYEAIGGRALYFGKPHARIYKLCFDRLGG
                     IARDRILAVGDNLETDIAGARAVGLATVL"
     gene            complement(737..1804)
                     /locus_tag="VGB88_08680"
     CDS             complement(737..1804)
                     /locus_tag="VGB88_08680"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9647559.1"
                     /translation="MTAWDAATAALRALPPEVAHRLTVRALGLGLGPAQAEPDPPALG
                     TRLWDLDFANPVGIAAGFDKDAEAHAAALRMGAGFIEVGTVTPRPQPGNPRPRVFRLA
                     EDEAVINRLGFNSAGAEAMARRLARRARGAGVVGVNIGINRDSDDPITDFESAFRRLA
                     LLADYVAVNVSSPNTPGLRALQQPAALDRLLAALQAARAALVAGGARRVPILVKISPD
                     LDADELAQVIGVARGRALDGLIVANTTLARPRELRSAARGEAGGLSGRPLFAPSTALL
                     AETWRLTEGRMPLIGVGGIASGGDAYAKVRAGASLVQLYTALIFQGPGLIARIKRELA
                     AHLAADGFARIADAVGTGAAS"
     gene            complement(1801..2154)
                     /locus_tag="VGB88_08685"
     CDS             complement(1801..2154)
                     /locus_tag="VGB88_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX9647560.1"
                     /translation="MYHLAREADWRRAQAGAPYLGAADCRRDGFIHFSTAGQLAASAA
                     RHRPGARDLVLVAVDAARLGPALRWEPGRGGALFPHLYAPLPMAAVVATVPLPLGPDG
                     RHLFPADIPSDPERE"
BASE COUNT          287 a          782 c          835 g          254 t
ORIGIN      
        1 agcaccgtcg cgaggccgac cgcgcgcgcg ccggcgatgt cggtctcgag gttgtcgccg
       61 acggcgagga tgcggtcgcg ggcgatgccg cccaggcggt cgaagcacag cttgtagatg
      121 cgcgcgtgcg gcttgccgaa atagagcgcc cgcccgccga ttgcctcgta cgcctcggcg
      181 agcgcgccgg cgcacagcac gcgctcgccc gactggcgca ccaccacccg gtcggggttg
      241 gcgcatacca ggacgagccc gcgcgcggcc gccgccttga gcaacggatg gtatttctcg
      301 acgctgtcgg actcatcctc gatgccgatg gcgagcacga agtccgcctc tgccatgccc
      361 gaaggcgcgt agtcgaggcc gtcgaggagt gcgcggtcgc gctcgggccc gaggtagagg
      421 tagcggcggc cgaagccggc aacggcgggg tcggtgcgcg cctccagcgc ttggcgggtc
      481 gcctcgcccg aggatacgat gcggtcgtag aggccggcct cgatgccgat ctcggcgagg
      541 cgggcgacga cctgggagtc gcgccgcggc gcgttcgaca gcagcaccac gcgctttccc
      601 cgccggcgca gctcggccag gcagtcgcgc gcgccgggat agggcgcggt gccgtccatc
      661 accacccccc acacgtcgag gatcaggccg tcgaagcggt cctcgagctc ggacaggcgt
      721 tgcaccagct cgatggtcat gacgccgcgc cggtgccgac cgcgtcggcg attcgggcga
      781 agccgtcggc ggcaagatgc gcggcgagct cgcgcttgat gcgcgcgatc agccccggcc
      841 cctgaaagat aagcgccgtg tagagctgga ccagcgaggc gccggcgcgg accttggcgt
      901 aggcgtcgcc gccgctcgcg atgccgccga cgccgatcag cggcatgcgc ccttcggtca
      961 gccgccaggt ctcggcgagc agcgcggtcg agggcgcgaa cagcggccgg ccgctcaagc
     1021 cgcctgcctc gccgcgggcg gcactgcgca gctctcgcgg ccgcgcgagc gtggtgttgg
     1081 cgacgatcag cccgtcgagc gcgcgcccgc gcgcgacgcc gatgacctgg gcaagctcat
     1141 cggcgtcgag gtcgggggaa atcttgacca agatcggcac ccgccgcgcg ccgcccgcga
     1201 ccagcgccgc gcgcgcggcc tgcagcgcgg cgagcagccg gtcgagcgcc gccggctgct
     1261 ggagtgcgcg caggccgggc gtgttgggtg aggacacgtt gaccgcgacg tagtcggcga
     1321 ggagcgcgag gcgccggaac gcgctctcga agtcggtgat cgggtcatcg ctgtcgcggt
     1381 tgatgccgat gttgaccccg accacgccgg cgccgcgggc gcgtcgcgcc agtcgccgcg
     1441 ccatcgcctc ggcgccggcg ctgttgaagc cgaggcggtt gatcaccgcc tcgtcctcgg
     1501 ccaggcgaaa gacgcgcggg cgcggattgc ccggctgcgg gcgcggcgtc accgtgccga
     1561 cctcgatgaa gcccgcgccc atgcgaagcg ccgccgcgtg cgcctcggcg tccttgtcga
     1621 agccggcggc gatgccgacc gggttggcga agtcgaggtc ccacaagcgc gtgccgagcg
     1681 ccggcgggtc aggctccgcc tgcgccggcc cgaggccgag gcccaacgcg cgcaccgtca
     1741 ggcgatgcgc gacctcgggc ggcagcgcgc gcagcgccgc ggtggcggcg tcccaggccg
     1801 tcattcgcgc tccgggtcgg aagggatgtc agcggggaac aggtggcggc cgtccggacc
     1861 gagcggcaac ggtacggtcg cgaccaccgc cgccatcggc agcggcgcgt acaggtgcgg
     1921 gaacagcgcg ccgccgcgcc ccggctccca tctgagcgcc ggcccgagcc gggccgcgtc
     1981 caccgccacc agcaccaggt cgcgggcgcc ggggcgatgg cgggcggcgc tggcggcgag
     2041 ctggccggcg gtcgagaaat ggatgaagcc gtcgcgccgg cagtcggcgg cgcccagata
     2101 cggcgccccg gcctgggcgc ggcgccagtc cgcctcgcgg gccaggtggt agatcgtc
//
LOCUS       DASWHU010000239         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_859464, whole genome shotgun sequence.
ACCESSION   DASWHU010000239 DASWHU010000000
VERSION     DASWHU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859464"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VGB88_08690"
     CDS             complement(<1..1471)
                     /locus_tag="VGB88_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9647561.1"
                     /translation="MRLVGVDVGGTFTDLVFTDTERAVTLIHKVPTTPDDPSVGAIAG
                     LGELCARNRLDPDSIDHVFHGTTIATNAVLEHDGATTGMITNQGYRDILHIGRHQRPQ
                     NYSIMQDIPWQDRPLVLRRHRRVVRERLVPPDGAVLEPLDEDAVRAAARAFKAEGVEA
                     VAVCFLFSYLDSRHEARAKEIVREEYPGAFVTTSAEVAPQFREFERFTTAAMNAFVGP
                     KVRRYVANFAAGLGQAGLEADLHIMGSHGGVATAAMVAERPVLTLLSGPAAGVLGGAW
                     EAAASGRDRLITFDVGGTSADIGIVTAGRFSEATARDTWIGGYPVLVPMIDITTIGAG
                     GGSIAHVDSGGAFRVGPHSAGSRPGPAAYGLGGEAPTVTDANLVLGRLDPDNFLGGEM
                     TLDVDAARRAIAGLAGRLGLGELEAAEGVLTILNSNMANAIRARTVQKGLDPRGFALV
                     AFGGAGPLHGAEVAALLGIPEVIVPLHPGITSAAGLLTTD"
     gene            1633..2538
                     /locus_tag="VGB88_08695"
     CDS             1633..2538
                     /locus_tag="VGB88_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX9647562.1"
                     /translation="MAKEIFCAFGVDVDAVGGWLGSYGGEDSPDDISRGMFSGEVGTP
                     RLLKLFDRFGIKTTWFVPGHSMETFPSQIREVVKAGHEIGIHGYSHENPIAMTRQQEE
                     AVLEKCIELARKLTGKRPTGYVAPWWEFSPITNELLLKKGFKYDHSLMHKDFEPYYVR
                     VGDSWTKIDYSKNARDWMKPLVRGRETDLIEIPASWYLDDLPPMMFIKAAPNSHGFVN
                     PRHLEEMWRDQFDWVYREYDYAVFAMTIHPDVSGRPQVLLMLERLYTHMIRHPGVRFT
                     TFDDIASDFAKRSPRKAKARAKRKT"
     gene            2550..>2733
                     /locus_tag="VGB88_08700"
     CDS             2550..>2733
                     /locus_tag="VGB88_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647563.1"
                     /translation="MCGLCGVLGAEEHWTDSAADGGSETRTRRRERLGRVALANRVLA
                     HYGLKLADFQGRAYVLR"
BASE COUNT          418 a          967 c          963 g          385 t
ORIGIN      
        1 ggtcggtggt gagcagcccg gcggccgaag tgatgccggg atggagcggc acgatcacct
       61 cggggatgcc gagcagcgcc gccacctcag cgccgtgcaa cggccctgcg ccgccgaacg
      121 cgaccagcgc gaagccgcgc gggtcgagcc ccttctggac ggtgcgggcg cggatcgcgt
      181 tggccatgtt gctgttgagg atggtcagca ccccttccgc ggcctccagc tcgcccagcc
      241 cgaggcggcc cgcgagcccg gcgatcgccc ggcgcgcggc gtccacgtcg agcgtcatct
      301 cgccgccgag gaagttgtcc ggatcgaggc gccccagcac caggttggcg tcagtcacgg
      361 tcggcgcctc gccgccgaga ccgtaggcgg cgggaccggg gcgcgagccg gcgctgtgcg
      421 ggccgacacg gaacgcgccg cccgagtcca catgggcgat gctgccgccg ccggcgccga
      481 tggtggtgat gtcgatcatc ggcaccagta ccgggtagcc gccgatccag gtgtcgcgcg
      541 cggtcgcctc gctgaaccgc ccggccgtga cgatgccgat gtcggcgctg gtgccgccga
      601 cgtcgaaggt gatcagccgg tcgcggcccg aggccgccgc ctcccaggcg ccgccgagca
      661 cgcccgcggc ggggcccgac agaagcgtca gcaccggccg ctcggcgacc atcgccgcgg
      721 tcgcgacccc gccatgcgag cccatgatgt gcaggtccgc ctccagcccc gcctgcccga
      781 ggccggcggc gaagttggcc acgtagcggc gcaccttggg cccgacgaag gcgttcatcg
      841 ccgcggtggt gaagcgctcg aactcgcgga actggggcgc gacctcggcc gacgtggtga
      901 cgaatgcccc cgggtattcc tcgcgcacga tctccttcgc ccgcgcctcg tggcgcgaat
      961 cgaggtagga gaacaggaag cacaccgcca ccgcctcgac gccctcggcc ttgaacgcgc
     1021 gcgccgccgc gcgcaccgcg tcctcgtcca agggctccag caccgcgccg tccggcggca
     1081 ccagccgctc gcgtaccacc cggcggtggc ggcggaggac cagcggccgg tcctgccacg
     1141 ggatgtcctg catgatcgag tagttctgcg gccgctggtg gcggccgata tggagaatgt
     1201 cgcggtagcc ctgattggtg atcatgccgg tggtggcgcc gtcgtgctcg agcaccgcgt
     1261 tggtggcgat ggtggtgccg tggaacacgt ggtcgatgct gtcggggtcg agccggttgc
     1321 gcgcgcacag ctcgccgagc ccggcgatcg cgcccaccga gggatcgtcc ggggtggtcg
     1381 gcaccttgtg gatcagggtc accgcgcgct cggtgtcggt gaacacgagg tcggtgaagg
     1441 tgcccccgac atcgaccccg accagccgca tgcgtccctc ccgcactcct cgcccgccag
     1501 cagtctagca gcccgcccgc ccggcggcca tccggcgcgc ggcacggttc cgtttgcgcc
     1561 cgcccgccgc taaagtagcg tcggccgtgc ccgcttgggc gggcaggaac acatcacggg
     1621 ggaggctgac agatggccaa ggagatcttc tgcgcattcg gcgtcgacgt ggatgcggtc
     1681 ggcggctggc tcggctccta cggcggcgag gactcgccgg acgacatctc gcgcggcatg
     1741 ttctcgggcg aggtcggcac gccgcgcctg ctcaagctgt tcgaccggtt cgggatcaag
     1801 accacctggt tcgttcccgg ccactcgatg gagaccttcc cgagccagat cagggaggtg
     1861 gtcaaggccg gccacgagat cggcatccac ggctactcgc acgaaaaccc gatcgcgatg
     1921 acgcgccagc aggaggaggc ggtgctggag aagtgcatcg agcttgcgcg caagctgacc
     1981 ggcaagcgcc cgaccggcta tgtcgcgccg tggtgggagt tcagcccgat caccaacgag
     2041 ctgctgctca agaagggctt caagtacgac cacagcctga tgcacaagga cttcgagccc
     2101 tactacgtgc gggtcggcga cagctggacc aagatcgact attcgaagaa cgcgcgcgac
     2161 tggatgaagc ccttggtgcg cggccgggag accgacctga tcgagattcc ggcgagctgg
     2221 tatctcgacg acctgccgcc gatgatgttc atcaaggccg cgcccaacag ccacggcttc
     2281 gtcaacccgc gccatctcga ggagatgtgg cgcgaccagt tcgactgggt ctatcgcgag
     2341 tacgattacg ccgtgttcgc catgaccatc caccccgacg tgtcgggacg gccgcaggtg
     2401 ctgctgatgc tcgagcgcct gtacacccac atgatccgcc atcccggggt gcgcttcacc
     2461 accttcgacg acatcgccag cgacttcgcc aagcgcagcc cgcgcaaggc caaggcgcgg
     2521 gccaagcgca agacgtgagc tagcgcgcga tgtgcggcct gtgcggggtg ctcggcgccg
     2581 aggagcactg gaccgacagt gccgcggacg gcgggtccga gacgcggacc cgccgccggg
     2641 agcggctcgg ccgggtggca ctcgccaacc gcgtgctcgc ccattacggc ctcaagctcg
     2701 ccgacttcca gggccgcgcc tacgtgctgc gca
//
LOCUS       DASWHU010000240        41674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_502107, whole genome shotgun sequence.
ACCESSION   DASWHU010000240 DASWHU010000000
VERSION     DASWHU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 41674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41674
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502107"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..647)
                     /locus_tag="VGB88_08705"
     CDS             complement(18..647)
                     /locus_tag="VGB88_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9647564.1"
                     /translation="MRLYDFAGAPNPRRVRIFLAEKGIEVPVEQVDLRARAQHEPGYV
                     ARNPSATVPMLELDDGTCIAESVAICRYFEALQPEPRLMGTDARDQALVEMWQRRMEI
                     DGFMAAGEAFRNATPGFKDRALAGTAKVAQIAALAERGRERLDRFFKFLDKVLAGRDF
                     VAGSRYTIADITALVTVDFAGWSKLKPGPELGNLAAWYERVASRPSAKA"
     gene            complement(673..1305)
                     /gene="thrH"
                     /locus_tag="VGB88_08710"
     CDS             complement(673..1305)
                     /gene="thrH"
                     /locus_tag="VGB88_08710"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEX9647565.1"
                     /translation="MYVACLDLEGVLVPEIWIGLAERTGIDALRLTTREVADYDALMR
                     HRLKALDEHGLGFADIRAAAEALAPLPGAVAFLDWLRVRCQVAILSDTFYELAAPLMD
                     QLGRPMLICHTLAIDAGGRITGYRLRQRHPKRRAVAAFQSLNFKVVAIGDSYNDTRML
                     ARADTGILFRPPDNVAEEFPAFPVCRSYDELRMAIEAAMGAQPEAACAAG"
     gene            complement(1436..2308)
                     /locus_tag="VGB88_08715"
     CDS             complement(1436..2308)
                     /locus_tag="VGB88_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008545563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9647566.1"
                     /translation="MSDRPVRARRSLIFAPGDKPAMYPKALATGADMVCVDIEDAIAP
                     HDKDAARARTLALFAEPQADDGVERIVRINSLRTADGLKDISAILESPTPPPALMVPK
                     VKCADEVRLLADLLAGAACADIRLHVIIETNDGLDAAFDIARASARMDSLLFGAVDMA
                     AELRTTLAWESLLYARSRLVHAAAGAGLDLIDVPFLDLEDPEGLAEEARRAAALGFTG
                     KAAIHPRQIAPINRAFSPRAEEVERARRIIAAFEQSRTGLVVVDGKLIERPVLRSMYR
                     TVAIAERIGASGMG"
     gene            complement(2324..2866)
                     /locus_tag="VGB88_08720"
     CDS             complement(2324..2866)
                     /locus_tag="VGB88_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647567.1"
                     /translation="MVDEPDFPYLCAAIFDDVGAFRGVLRSLLGAGFDAADVSILAPH
                     DALADHFDDRIPSPDQLADLPDTPREDLAPETAVEAAARAIAEGLSAIGAIGAAGMAY
                     AVGGPVGVATTTGAAVESTVEGVLSKFVEDRYSERFQRNLADGGLVVWVRVRERERAA
                     TASALLARHGGSHIHAVAAP"
     gene            complement(2984..3298)
                     /locus_tag="VGB88_08725"
     CDS             complement(2984..3298)
                     /locus_tag="VGB88_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647568.1"
                     /translation="MSILRLAAAGTLLAGACLSALPASATFVHMVGRTWMIEQWIKAE
                     FDGSVVELTPIMHHGNPAFRVSFIPGGASGASTAPPRTLIIDAGSMQPVEEIEDDAVP
                     RP"
     gene            complement(3376..3993)
                     /locus_tag="VGB88_08730"
     CDS             complement(3376..3993)
                     /locus_tag="VGB88_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9647569.1"
                     /translation="MADLKPGARGVPYDQRLGALLVQTLARTPIAPNHLTGLSFALGA
                     LAGWTLAQGRFGLGAGLFMLAVFIDHLDGELARATRRTSRFGHYLDYAVGAANYTMMY
                     LGAGIGLAAGELGSAALTLGLAAALSNPAVLAVRLANETRHGSEAVAHPYFHGFEIED
                     FIYLIGPFAWLGWFDVFFLAYALGALGYLAWQTWELVRRVRATAP"
     gene            complement(4010..4999)
                     /gene="hpnA"
                     /locus_tag="VGB88_08735"
     CDS             complement(4010..4999)
                     /gene="hpnA"
                     /locus_tag="VGB88_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX9647570.1"
                     /translation="MTALITGATGFVGSALARCLLARGMALRALVRASGDRRNLDGLE
                     VELVEGDLTDPPSLAAAVRGCEALFHVAADYRIWCRDPSAMMKVNIEGTRALMRAAAD
                     AGVKRIVYTSSVAALGAKADGSAADEEQPATLDDMIGPYKRSKFLAEAAVRELARSEG
                     LPAVIVNPSTPIGPRDIRPTPTGRMVVEAATGRIPAYVETGLNVVHVDDVAEGHWLAF
                     ERGLPGRRYILGGEDMTLRDILAEVALLVGRRPPRLRLPHAAVLPVAYLAEAAARVTG
                     REPMTTVDGARMARKPMYYSSRRARDELGYRPRPAAQALSDAVAWFRDHGYLR"
     gene            5105..5884
                     /locus_tag="VGB88_08740"
     CDS             5105..5884
                     /locus_tag="VGB88_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9647571.1"
                     /translation="MTHTNVTRLEELRRETLDTGVQDERTLRVQLAAAYRIFDYFGWP
                     QLIFGHITVRLPGPDRHFLINPFGLLYHEVTASNLVKIDLDGNIVGSGEYPVNPAGFV
                     IHSAIHGAREDAHCVMHTHTRAGMAIAALKDGLNCSDFAGISLYNRVAYHDFEGLTVR
                     MDERERLVRSLADKNVLVLRNHGLLTCGKTIAEALLRMCTLQTACEVQLTARAAGGAL
                     VEPSPEVCERHAASFDSGNQSARVFAALMRLMDERDPSYRN"
     gene            6074..6655
                     /locus_tag="VGB88_08745"
     CDS             6074..6655
                     /locus_tag="VGB88_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9647572.1"
                     /translation="MSQIAVTVSSRSSPGYRFDRLSVMVVDDNRHMLNLIAEILRGLS
                     IRRLIRLTDPADAFKELLIAPVDIIVVNHVMEPLTGIEFAKLVRFGDDSPDLFVPIIM
                     VTGYSDAATVRAARDAGVNEFLAKPISVRGLYTRILEVIDHPRPFIRCPDFFGPDRRR
                     QVVPFDGQDRRVSAPSESPEGGAAQLIPVAART"
     gene            6732..7208
                     /locus_tag="VGB88_08750"
     CDS             6732..7208
                     /locus_tag="VGB88_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX9647573.1"
                     /translation="MPNKIKDKVTLGGRIKGDGFDPKLLQRAERRVEAMRDVFLDAAR
                     DDIDRLVAALSNAQTDAARRGRHLDTLEKIAHEIKGYGSHIGYDLLTRFADSLSLFLR
                     KAKTTVAVKLEVAEVHVDAMRLVYHDGLTGDGGEQGADLTAGLRSAVGRYLRAEDF"
     gene            7348..7803
                     /locus_tag="VGB88_08755"
     CDS             7348..7803
                     /locus_tag="VGB88_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9647574.1"
                     /translation="MRDTTHAPELKTDRLIASGFELSGRWELDTASRARFIGEAPKQP
                     GVYAFAVDGKVLYIGSAQRGIAKRLRRYEITEIRLTAFRVRALITEALGDGSEVAVLT
                     IVPCSMRWKGLPVDLIAGLEEGLIRELRPEWNIRGLGALRKKTASEISN"
     gene            7884..8174
                     /locus_tag="VGB88_08760"
     CDS             7884..8174
                     /locus_tag="VGB88_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647575.1"
                     /translation="MASRTTSRGAAPVTLGELAAEGRRVWLWCERCARSRSIAATVLA
                     RRLPADLPVPLIGRRVRCRICGGREVHSRPHWPPGETVASHRWHDGGSGAEE"
     gene            8283..9482
                     /locus_tag="VGB88_08765"
     CDS             8283..9482
                     /locus_tag="VGB88_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647576.1"
                     /translation="MNKATTATRTPGLGVDEAERAELHRYIRHIDRALNRLWNLGRNL
                     VVGWHPAEAGLRVLVIPHHMLRELAEQDGDLLCDGHRVTHARLDALGRQLGIEPIQVR
                     LPNRPGRPGIAHKAIESVVRRYSVTKSNHRAAILLDIVGFSLYSPLEQVTLLNSLAFS
                     INVAQSRALANKLRIDVSRSTTGDGFYVWNRDEGLEADVNLFYLMMLVLADNALARHK
                     GAPSAVPVLRSCFHLGSHYEYYPADGLNPSVTGFIVGDLTIELARMVGKALPGQVLIG
                     QFTRPKAGVGGHAGALSTLAFVSRAQDRLDQLHALELSGQRITAIKCYLTGKRIGGGV
                     YSITKYALSDKHGRQRNVFNAKVNIYRGDADVLYLGLENKDLERFDAEAAEPRTRARP
                     GVRASVD"
     gene            9504..11093
                     /locus_tag="VGB88_08770"
     CDS             9504..11093
                     /locus_tag="VGB88_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9647577.1"
                     /translation="MTTLAAAETACRAALAADPNDADALDQLARIQLERAPEQALSHL
                     LHAHAARVGGRHAEAVRAAREALRRAPADVEALNLLGLSLRDLGDLDQAVAVWQRAVR
                     TDPALADLWLNLGKALCEAGRAEDALAPLAEALSRRPDHGDSLLTLGNALIALERAPE
                     AAAAFECGLAGDPRSARLHANLALARRQCGDLDGAEHHLRQAVALAPALAEAHKNLAM
                     LDLLRGRFADGFDRFAWRWREQRAFNRPRPLPQPPWDGSALGGRTILVWGEQGVGDEI
                     MFASAYPEVIAAAARCVVECEARLVPLFQRSFTGARIVARTEPPAAELADPAIDLQCA
                     AGDLCRWLRRSREDFPVPAPYLLADPGRIAAAVARVRALGDGPKVGLAWRSRTPVWGA
                     AKSAPLALWDPILSVPGPVWIDLQYGDCADERAAARARLGVTVHHDPSVDQMASLDDF
                     AAQVAALDLVITTSNTTAHMAGALGVETWLLLPHMPDWRWQATGEDSLWYPRTRLFRQ
                     PSPGDWRTPIESVAAALRGRL"
     gene            11146..12474
                     /gene="hemN"
                     /locus_tag="VGB88_08775"
     CDS             11146..12474
                     /gene="hemN"
                     /locus_tag="VGB88_08775"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX9647578.1"
                     /translation="MTDLVAKYGLARVPRYTSYPTAPHFDSRIGPAAHHDWLGALDAG
                     TRVSLYLHVPFCARLCWYCGCHTTITRDPERVHRYADLLARELDLVRAALPARLAAGH
                     VHWGGGTPTMLGAGGFTRIAALVRDRFALTADAEIAIEIDPRSLDAETARALAGAGVT
                     RASLGVQDFTSRVQQAVNRVQPFEQVARAAACLRAHGIRALNFDLMYGLPHQTAADVG
                     RSVDLAVRLAPDRVAVFGYAHVPWLKRHQRLLDEAALPGAAARRVQAETAAARLIAHG
                     YVAIGLDHFARPGDALARAAAEGRLRRNFQGYTTDDAPALIGLGASAISTLPQGYAQN
                     AGDLRQYRGRIAAGRLATARGVALDDDDRIRRRIIARLMCGFEADLGPADGALEVDRG
                     TLAEMARDGLVVGDGSRLRVTEAGRPYVRTVAACFDRYLSSRAGRHAQAV"
     gene            complement(12561..13013)
                     /locus_tag="VGB88_08780"
     CDS             complement(12561..13013)
                     /locus_tag="VGB88_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647579.1"
                     /translation="MGSGTVGGFVRRGRRVALAALAAVAAGLAGTTAAAAQAFEDPVA
                     ITVENATAKVGETTAVRATLTPREGFGLTDVYRHRIIKLSTDGGVAFEDEVVAGKMDD
                     GKVVFSVAVTPTYPGTHVINGVFRYSVYQEGHLEMKSAPLIATVTGTP"
     gene            complement(13181..14896)
                     /locus_tag="VGB88_08785"
     CDS             complement(13181..14896)
                     /locus_tag="VGB88_08785"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX9647580.1"
                     /translation="MKVAKYAALLGVGLMPAVAAAGMLSDYSTVTSQRLTNPEAGNWL
                     MTRGNYWGWMYSPLDQINTANAGSLKPVWSFSTGVDSGHEAPPIVNDGVMFVATPYAQ
                     VIALKAATGDLIWRYRRELPDDFGALHNTSRGVALYGNNVYLTGLEPTTLALDAKTGK
                     VVWETVVEDYHTGYYQTMAPLIVDGKVMVGVSGGEFGVRGFVQALDAETGKPAWKTFT
                     IPGPGEPGNDTWPGDTWKRGGGAVWNTGTYDPELGLTFWGAGNGSPWFGDQREGDNLY
                     TSSTIAIDPKDGSIKGHFQYHWNDSWDWDEMNAPTVVNFEHGGKMVKGLIKPARNGYL
                     YWLERKANGIGFVDAKAYVKQDVFKSIDPSTGRPTYNDDRKPGTGKYAGFCPSLWGGK
                     DWPFEAYNPKTGLLYIPANENHCGFLEGKVQEYVAGQWWTGVDIPDIGFVVEKSARHF
                     GEIQAWNLNTGEQVWSQLYPGMMNWGSLLTTGGGLVFNGGTNDRYFRAYDARTGEALW
                     GFRTNSGIMAPPASYEVDGVQYVAVASGWGVDPAFQQGLMNNLLGTDTVVPQGGVIWA
                     FALGE"
     gene            complement(14963..15541)
                     /locus_tag="VGB88_08790"
     CDS             complement(14963..15541)
                     /locus_tag="VGB88_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9647581.1"
                     /translation="MQAFGKARRALLRSGLGLGAALPFLKLAAVRAADNPEKMRPQPG
                     DRFVFLKGDKKGEVIKVDDLPLGGPQEIAYPIDPATGTVRKGSRLNQVALIRLADAAI
                     TDRTRRFAAEGVVVYSAVCTHQGCPVSMWQPENDTLYCSCHGSEYNPGDMAEVVAGPA
                     PRRLAILPVKVEDGVIVAAGEFEGAVGFKKPA"
     gene            complement(15627..17429)
                     /locus_tag="VGB88_08795"
     CDS             complement(15627..17429)
                     /locus_tag="VGB88_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9647582.1"
                     /translation="MKSHHKTSLAGALLGVSLAAAAAAGPVDDARLRAESPASGDWLS
                     YGHGYAHHRFSSLDQIDRTSVARLVPRWIYQTGISATFQATPVVADGIMYLTTPRNDV
                     VALDAASGEQVWRYRHQMVTDKLCCGPANRGAALGYGAVYMATADGRLVALDQATGAV
                     KWDVTIAAPDTRPTETLSELAADDPLRGQRVTGASGLGANMAPLVFDGLVIAGVTGAG
                     YGLHVDDAEGNQLGAVIGFEGQFGRRGYLAAFDAKTGAEAWRWYTTKADGWEGEWRTH
                     TPDGVALERDIAAERARQAEYADAWQVGGGSTWSTPSLDPELGLLYLGTGNPAPQMDD
                     VSRPGDNLYTVALVALDARSGELRWFYQQVPHDLWGYDVGTPPVLVDLARDGGTVKAV
                     ASASKTGWMYVLDRATGELLFKSEPFVPQSNMFRRPTGEGVTIAPGAGGGASWSPASF
                     DPRTGLAYLAALHMPTTYSVRERPATADKPARRYSVLEVANEPRWGTLSAIDLADAGR
                     IRWQVKSDDVLLGGVLATAGDLVFMGEGNGYLNAYDSATGARLWRFQCGAGVNAPPVT
                     YQVDGIQYVAVAAGGHHLFGFPQGDAVIAFALAP"
     gene            17680..18489
                     /locus_tag="VGB88_08800"
     CDS             17680..18489
                     /locus_tag="VGB88_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9647583.1"
                     /translation="MPASDGAGVRLSRIIGQPGLDMVDPFLLLDEFKSDRPEDYIAGF
                     PEHPHRGFETVTYMIAGRVRHADNKGHAGVIEAGGVQWMTAGRGIVHSEMPEQEDGLL
                     WGFQLWVNLPAADKMKPPAYQEFPAERIPAESRDGGVVVRVIAGTTAPGTTGPVTGIP
                     TAPAYLDVALAAGSRFEEPLPAASSAFVYPFQGKVRVAGNGAATELAAGTLGLLGPGD
                     VVRIEAGDDDARLLLVAGRPLGEPVARYGPFVMSTKAELTRAYQDFQAGRF"
     gene            complement(18508..19284)
                     /locus_tag="VGB88_08805"
     CDS             complement(18508..19284)
                     /locus_tag="VGB88_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9647584.1"
                     /translation="MTDTVLSARPADGVLQLTLNRPEARNALSRAVIHRLAEELAWAD
                     ADAAVKCAVLTGGAKVFSAGADIKEFTDAGGPEVLDDGRRNADWAAIERFAKPLIAAV
                     EGYAFGGGHELMLVCDIIIAAADARFGQPEIKIGVLPGDGGTQRVTRIAGKPLAMWMM
                     LTGEPIGAEDALRAGLVSKLVAPGTALAEARALAERLARLPPVALALAKQAVLAAYAT
                     GLPAGLAFERQAVLRAFATEDRIEGMTAFRDKRAPVFKGR"
     gene            19433..20320
                     /locus_tag="VGB88_08810"
     CDS             19433..20320
                     /locus_tag="VGB88_08810"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647585.1"
                     /translation="MNESQPKLGYIGIGLMGTPMARRLIEAGYDVTVWGRSPAKIEPV
                     LAAGAHRADDAAGVARAADIVLTCLSDTAAVDAVVFGKGGIAAGAGPGKVLVDMSSIR
                     PDAAREMAARLKAETGMGWIDAPVSGGVAGSESGNLTVMAGGAQQDFDRVAPVVAHLA
                     KRFTLMGPNGAGQATKLINQVLVGCTVAVLSEAASLALRAGIDAERIPQALAGGRADS
                     LPLQQFFPKMVKGDFFVESHLRTMLKDLETVQALARETTTAMPMTGMATELHRLMIQR
                     GFADRDGTALAMLYRADPI"
     gene            20341..21534
                     /locus_tag="VGB88_08815"
     CDS             20341..21534
                     /locus_tag="VGB88_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9647586.1"
                     /translation="MDILDGIRVLSFNHFLLGPVGVQVLGDLGADVIAIEPVEGAFQR
                     RWGGANRRVDDETVLFLCASRNKRSLAIDLKAPAAKEIVERLIAGADVLCENFRPGVM
                     DKLGFGYERARAITPSLVYASASGYGQDGPYRDRPGQDLIVQAMSGLMNINGSVDQPP
                     IPIGVSAADHHGAQVLAMGILAALVRRARTGRGCRVDVNLLSAALDLQMESLVAFANG
                     APVSQRPPAHIAGWYYPAPYGVYRTADGHIAISLASMTTLADALEMPALAEVSDDETF
                     TRNGDIASLIQERVAGVACAEVEAMLERHKLWYARVNDYTAVLNDPQVRHNGSVITTD
                     NAHGTPVRLLAHPVRYDGEVPPVRIPPQPLGAQTADILAELGYARDDIDRLAAAGVVR
                     VAGRA"
     gene            21646..22611
                     /locus_tag="VGB88_08820"
     CDS             21646..22611
                     /locus_tag="VGB88_08820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9647587.1"
                     /translation="MRRFPDFEPKGVIPATLMAFDDDFGIDEAGARKHLRDVAATPGL
                     AAVTVNGHASEVHACTFDEQQRILEFSLDEVGDRLPVINGVYADGSLEAARIARMSET
                     CGAACLLVFPPQSMIMGGQLRPEMALGHFKTIAAATDLPIIVFQYPMTSGLGYPFDTL
                     LRMVDEVPTIRAIKDWSNDAMLHERHTRTLQSLARPITVLTTHSAWLMSSLVMGAQGL
                     LSGAGSVIPDLQVALFEAVQAKDLARAQAINDRIYPLSQVFYAPPFLDMHNRMKECLR
                     LLGRLDKAVVRPPLMKLSEAEIARLQQALDEAAIAPEGALGLAAE"
     gene            22672..24387
                     /locus_tag="VGB88_08825"
     CDS             22672..24387
                     /locus_tag="VGB88_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9647588.1"
                     /translation="MTGLFDPGRIGPVEIPNRIVMPSMTTRTADEAGFVTDDTVAYFA
                     ARARGGVGLVTVEMSAPEPAGRHRLRELGIADDRFVPGLGRLVAAIHDAGARAAIQLG
                     HGGGHTRADVCGEPPIAPSAVPHQVFERTMETVVPVAMTRARIGQTTRAFVDAALRAQ
                     AAGFDCVEIHAAHGYLISQFLCPAENTRTDDYGGALENRARFGLDILRRVKAAAPGLG
                     VIFRLTVDDYFAGGMPAADGIRVAEWAAAAGADAIHVSAGHYRSLPATMMIPPMAMPE
                     ATFLHLAAAVKARVAVPVIAVGRLGDPALAKAAVADGKADFVALGRGLLADPDWPDKV
                     RRGVAPRHCLACLACIDGMRAGSRLHCIVNPTTGRERAFAEARPPAGERIAVIGAGPA
                     GLSYAELVAGANRVTVFERAKVLGGALRLAARAPLFENVAAAPASFAAYISALEAACR
                     AKGVDIRTGTDPMRDPRLLDGFDRVVIATGAGYRFWLRPVVRALLVTGLARLPGLRAL
                     LARPRLRDWFYTRARAGRGAALAGRAPPGQVVEVIGDARRAGKTGAAVRDAFEAALLR
                     PAVRG"
     gene            complement(24431..25582)
                     /locus_tag="VGB88_08830"
     CDS             complement(24431..25582)
                     /locus_tag="VGB88_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX9647589.1"
                     /translation="MTIEVQHVTKRFDRFTALDDVSLRIDPGELVALLGPSGSGKTTL
                     LRIIAGLDFPSQGRVLFDGEDASARHVRERQVGFVFQHYALFRHMTVFENVAFGLRVK
                     ARRERISEAAIRRRVLELLHLVQIDQFARRYPSQLSGGQRQRVALARALAIEPRVLLL
                     DEPFGALDAKVRKELRRWLRRLHDRMRLTSVFVTHDQEEALELADTVVVMEQGRIAQV
                     GAPAEIYDHPATPFVYEFLGDVNRFSCVIRAGTAVVCDTDFAIADAHGAPDGPAIAYV
                     RPDDVEIVLDKARLGLPAIVREVIRTGPLVSVELSIGGKRPVVEATLSRAEFEALGLE
                     GKQSVFIRARAARVFPLDARERPEQVVVTSDADQARESTPVSGTLPAQE"
     gene            complement(25594..26460)
                     /gene="cysW"
                     /locus_tag="VGB88_08835"
     CDS             complement(25594..26460)
                     /gene="cysW"
                     /locus_tag="VGB88_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX9647590.1"
                     /translation="MTTQFENLGRARVATESAGVRWALTALALAFLSLFLLLPLVTVF
                     AEALRAGVDAYLAAVTEPEAAAAIRLTLLAAAIAVPLNVAFGLAAAWAIAKFEFLGKS
                     VLTTLIDLPFSVSPVIAGLVYVLLFGAQGWFGPWLAEHGVQIIFAVPGIVLATVFVTF
                     PFVARELIPLMQEQGTEEEEAALSLGAGAWQTFWRVTLPNVKWGLLYGVLLCNARAMG
                     EFGAVSVVSGHIRGLTNTVPLHVEILYNEYDFAAAFAVASLLAFLALVTLALKAVLEW
                     RYGAELAATRRR"
     gene            complement(26611..27447)
                     /gene="cysT"
                     /locus_tag="VGB88_08840"
     CDS             complement(26611..27447)
                     /gene="cysT"
                     /locus_tag="VGB88_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX9647591.1"
                     /translation="MPSRVRRPSILPGFGPALGFTLLYLSLVVLVPLAAMVMRTAGMG
                     AADFLAAVFAPRALAAYRLSFGASALAALINAVFGVLVAWVLVRYRFPGKRIVDGVVD
                     LPFALPTAVAGISLAALYAENGWIGQFLAPLGMKVAFAPSGVVVALVFIGLPFVVRTV
                     EPVLQDFDVELEEAAASLGANRLQIFLRVILPSIAPAVLTGVALAFARAVGEYGSVIF
                     IAGNLPMVSEIAPLLIVIKLEQYDYAGATAIGTVMLLASFVLLLLINLLQHWSRVRMA
                     PA"
     gene            complement(27668..28651)
                     /locus_tag="VGB88_08845"
     CDS             complement(27668..28651)
                     /locus_tag="VGB88_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9647592.1"
                     /translation="MAAAVLLAPTPTWADRTILNVSYDPTRELYQEFNAEFASHWKET
                     TGETVTIRQSHGGSGKQARSVIDGLPADVVTLALAYDIDEIAARSGLIPKDWQLRLPH
                     NSAPYTSTIVFLVRKGNPKGVRDWDDLVKSGVQVITPNPKTSGGARWNYLAAWGYALR
                     KWSGNEGKAREFVGAIYRNVPVLDTGARGSTNTFVQRGIGDVLVAWENEAFLAVEEFG
                     PDKFEIVVPSVSILAEPPVTVVEANAERHGTLDLARAYVEYLYAPAGQRVAAAHYYRP
                     HEPQHARPEDLDRFPDLERFTVDEAFGGWQAAQRKHFADGGIFDAIYQTTQ"
     gene            complement(28724..29740)
                     /locus_tag="VGB88_08850"
     CDS             complement(28724..29740)
                     /locus_tag="VGB88_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647593.1"
                     /translation="MPAPAIAQGRVSWRMVTCWPRNFPGVGIAAQRIADRIGEMSDGR
                     LEVKLFAAGEFVPAFEVFDAVRDGNAELGHDSPYYWIAKNRSSAFFSSVPGGLTPDEH
                     MGWIYYGGGQALWDELYEGFGLRAWLAGNAGMQMVGWFRNQVRSLDDVKGLKIRMAGL
                     QAEVLNRMGATTVNLPGGEIMPALQSGVIDAAEWGGPWMDLAFGFYKVAKHCYGPGVH
                     EPGTAQSLMVNLEAYRALPKDLQAIVRNSATVEAALLLSEFDFENAVALQRLVAEHDV
                     TIGPLPDDVLKRWFEISAEVVAETAADGDINRRIYDSWSTYRNNAVAIAPLSTLGYMR
                     SRGA"
     gene            29962..30405
                     /locus_tag="VGB88_08855"
     CDS             29962..30405
                     /locus_tag="VGB88_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9647594.1"
                     /translation="MAEFPLKVGDQVTFAKTVGETDIYLFAGITGDFAGNHVNEEYMK
                     GSSYGRRIAHGALLIGYMSTASTMMINTCLRADMTETPVSLGYDRVRFTAPVFINDTV
                     TVTYRIKDTDPDSRRSRSQITVTNQTGETVAVAEHILKWVPNQRS"
     gene            complement(30377..31324)
                     /locus_tag="VGB88_08860"
     CDS             complement(30377..31324)
                     /locus_tag="VGB88_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009807012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9647595.1"
                     /translation="MPADIVCLGEPLAEFTRLPDSGGNRWLAGYGGDTSNCAIAAARQ
                     GARVGYVTALGADPFGESFLELWRAEGIVTSGVKRDPEAQTGVYFITHDTSGHHFTYY
                     RTGSAASRMRAADLPGNMIADAKVLHVSGISQAISASAYEAVAHAVALANESGVRVSY
                     DPNLRLRLWPLERARAVIDATAAQAWYVLTNAEEGRLLTGRERVAEIAAAYLDLGARV
                     VAVKMGAEGVLVATGAEHRRIAGHAVACVDASGAGDAFDGAFLAETVAGRDPLAAAAY
                     ANAAAALATIGYGAVAPIPRRAEVEAFLARARTAGSAPI"
     gene            complement(31333..31995)
                     /gene="eda"
                     /locus_tag="VGB88_08865"
     CDS             complement(31333..31995)
                     /gene="eda"
                     /locus_tag="VGB88_08865"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX9647596.1"
                     /translation="MNRAAPSADIGGLLGRLVEAKIVPVIRTSAATTAEQAVDALADA
                     GFDVFEITMTIADAPALIESLAARPGVLVGAGTVTSEQDAERCLSAGARFVVSPCVVP
                     AVVAPCHAAGVPVLLGALTPTEVLAARAAGADAVKIFPVSSAGGPAHVSALRSIFPGV
                     PLVPTGGIRAEQVLEYLDAGAAFIGMGGRLVDERLILAGRSGEITALGKSLRASLGLP
                     AG"
     gene            complement(32050..33231)
                     /locus_tag="VGB88_08870"
     CDS             complement(32050..33231)
                     /locus_tag="VGB88_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9647597.1"
                     /translation="MSRKTVRAGLVGSGFAAALHVHGIRRVYGTDVDLVGVYSPTPAN
                     ARRFADAHGLAALGSIEEVLDKSEVVHVCVPPAAHEPIAVAALARDRFAIVEKPLTGY
                     FGDGSADFDGRGAPKQAALAGALASIERMLAAEQASRGRILYAENWVHAPAIQKEREI
                     VEKTGAQILWLHGEEAHSGSHSKAYGFWRLGGGGSMIGKACHPLGAALYLKRVEGRAR
                     DGRPIRPATVSARTHELTRLEGYRDAGHLRTDYHDIEDFAAIHVTFEDGTVADIFASE
                     IVMGGIHNWLEVCANNHRAICNINPNTAMLTYNPVDATFKDIYVVEKTGTKQGWQFTA
                     PDEDQMTGFPQEIEAFYRTVAYGEEAESDSRLAADCISTIYSAYLSAERRGAEVPVRA
                     F"
     gene            33339..34583
                     /locus_tag="VGB88_08875"
     CDS             33339..34583
                     /locus_tag="VGB88_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647598.1"
                     /translation="MHGTVGLDRRGWARALAAAVVAALALAACSESDEDKATAAYERG
                     DFQAAGALAGALAAAGNPRGLELLALMAAQGLGRAIDYGEAFALIDRAIALNPSYADT
                     RAAIEARRTAATAASAKAFDTGDYERAYDIARPLAAFGDDDSARLVDALVTGHYIRLP
                     GSDMSWRRFWEQCSGNTRFETEAEAEAGFDSGCAGLHAVWDGLVVRALPGELHVKMTP
                     GRPAAHHDITLMLDPPPDPELASPGRKVRFAGVVAARGTPSRPDVLAQGAIIGPAPLT
                     AEEASHEQTVVLQAVMNACQTLVEERFRAELMPQWAIEVEAQVRAGGSPRSRAFSLFV
                     GITSGPDAFARQADGTWHGRFDGTVTIQSVVARTAQVTEYTADCTIDGAYRRGDRPGE
                     HGVLSFVRVAEPLVESAPARLR"
     gene            complement(34639..35091)
                     /locus_tag="VGB88_08880"
     CDS             complement(34639..35091)
                     /locus_tag="VGB88_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9647599.1"
                     /translation="MIGAASASLADFDAGNRAYAVRDYETAFKEWLPLAEAGDATAQN
                     NIGFMYRKGRGVPPNEKEAVIWYRRAAEQGLPDAMTNLGYMYDEGRGIEQDLIESYKW
                     FLLAAERGREGAEGHLDVLKDFLTPEQIAEAEKRAAAWTPSPEQGERN"
     gene            complement(35178..35675)
                     /locus_tag="VGB88_08885"
     CDS             complement(35178..35675)
                     /locus_tag="VGB88_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9647600.1"
                     /translation="MALAGLLAMAWIAPASAQMQRRLPGAAIYKQAGCITCHKWHGMG
                     GPGYGGTPINFRQTILELDQMIEVISCGRPGTGMMYHLRGAYKDHDCYDGMTLEELGE
                     MAPGPARQLLNHRQVKHVAEFIVEHFKGRSDDLETADCELFFGQSKMCRQLKLDVAGG
                     GGGGH"
     gene            complement(35863..36348)
                     /locus_tag="VGB88_08890"
     CDS             complement(35863..36348)
                     /locus_tag="VGB88_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEX9647601.1"
                     /translation="MTDAGRRAFGRREGKTMTRTWRRAASVALALLVLWLGAPVRADF
                     ISGKHAYDAGDFNTAHQEWLAAALAGDAQAQYELGIMLANGVGEPRDVISAYAWLTLA
                     AKGGVKDAKEKYDLLLKEYIPRHCHFDALALVRSFESGQPERLAEGGRQNSRCWNFKQ
                     N"
     gene            complement(36384..36617)
                     /locus_tag="VGB88_08895"
     CDS             complement(36384..36617)
                     /locus_tag="VGB88_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647602.1"
                     /translation="MRKSVIALIAAAATAGFAVSGVAAGPSAEEQTSAEQVEMCTTGL
                     QQCQSGNSMGCVTALKTCVGEDRKKAMSLMEQS"
     gene            36958..37632
                     /locus_tag="VGB88_08900"
     CDS             36958..37632
                     /locus_tag="VGB88_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647603.1"
                     /translation="MTPDAAFRRAGAGGLLAVAAAVLAYGGPVGAGQDGDIREFTVGL
                     TVDELPATGYLGFKCGNNGAKPGREIAGWTAFAECPPDDKGLHEVAFEYDDSEVRFKD
                     LEGTAIAGHEVLISLLFNDQGQVEAIRVFTNPFARPYEKRRAHILANKVKHRFGSEGW
                     TCVNQQLADGESDIAGVFIKESCRKDLGNRFIDLHTSFFRRHGPEGETIVNATSFEIW
                     REAAPS"
     gene            complement(37750..39567)
                     /locus_tag="VGB88_08905"
     CDS             complement(37750..39567)
                     /locus_tag="VGB88_08905"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX9647604.1"
                     /translation="MKEKVTKPAATLAAVGAGLLALAMSLPASAAEMTTADDLLNATK
                     NADDWLTVHRTYNSDRYSPLDQINKSNVKDLKVAWAIALGGTEGGGFWRYGNIEGTPI
                     VESGTMYMANGWSELMAIDLRKAGQGPDMVKWTYDPGVDKDWAGNVACCAINNRGVAV
                     WGDSVIMNTLDGRMLRIKKDTGELVWETQLADPAVAETVTIAPLVVKDMAITGVSGAE
                     YGIRGWLAAVDLNTGNEVWRRYTIPGPGEPGHETWKDDNDGWLTGGGSTWQTGSYDPD
                     TNLLYWGVGNPGPDWDNEYRPGDNLYTSGMFALDADTGKVAFHFQYTPNDPYDYDGIN
                     ENILVNVNIGGNDRKAFIHADRNGFFYAIDRTNGEFIYGIPFVKEVNWTRGLDKVTGV
                     PLDYNPNSDLQVYVAEHTPNRTNKSATFCPNLMGGKNWMPMSYSPRTKMAYIPTIESC
                     ITVNNEEETPGPEGSYVLREWFSGGMPDVTTAKSSDGKSLAGSLTAVDVTTGRIVRKV
                     DTRYPVMAGVLSTAGGLVFVAQHEGKFCAFDDESLDELWCFHMNTLTDAPTMSFAVDG
                     KQYIAVSSGGWGTVPTYFAPSTPGLEKIRNANVLWVFAL"
     gene            39894..40946
                     /locus_tag="VGB88_08910"
     CDS             39894..40946
                     /locus_tag="VGB88_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9647605.1"
                     /translation="MESHPIDCAPALPERFEDIEALEDVLSAPTAQLVEDLGRLDGDV
                     LVLGVGGKMGPTLARLARRAAPAKRVVGVARFTDKALRARLESWGVETIACDLLDRAA
                     LAALPRLANVVFMAGRKFGATAAAADTWAMNALVPAAVAEAFAASRIVAFSTGCVYPY
                     LPAAGGGATEKTAPSPPPGDYAWSCLARERLFEYASRRFATAGRLIRLNYAIDMRYGV
                     LHDIARAVFAGAPVDLATGHVNVIWQGDANAQALRALAHCTSPTSPLNVTGPETVSVR
                     ALAHAFAERFGKPPRFAGTESATAWLSDSTAARRLFGAPRVPLSRMIDWTADWTLRDG
                     ASYDKPTRFEVRDGRF"
     gene            40946..>41674
                     /locus_tag="VGB88_08915"
     CDS             40946..>41674
                     /locus_tag="VGB88_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9647606.1"
                     /translation="MDVLAPHTLPARLDDEAALEDLLSTPSQALIDDLARLDGDIAVL
                     GVAGKVGPTLARLAKRAAPDKRVIGVARFSQPEIRERLESWGVETIACDLLDRDQVAA
                     LPKPANVVFMAGRKFGTTGDEPLTWAMNVLVPAIVAEAFAASRIVAISTLCVYPFTPV
                     ADGGAGEDTVCAPPGEYANSCIGRERAFEYAAAVHGTRGRLIRLAYAIDMRYGVLHEI
                     ASRVRAGEPVDLATGHLSLIWQGDA"
BASE COUNT         6205 a        14975 c        14214 g         6280 t
ORIGIN      
        1 gcttcggcgg agtaggctca cgccttggcg ctcggcctgg acgcgacgcg ctcgtaccag
       61 gccgcgaggt tgccgagctc cggcccgggc ttgagcttcg accagccggc gaagtcgacc
      121 gtgaccagcg cggtgatatc ggcgatcgtg tagcgtgacc cggcgacgaa atcgcgtccc
      181 gccagcacct tgtcgagaaa cttgaagaac ctgtcgaggc gctcgcggcc gcgctcggca
      241 agggctgcga tctgcgccac cttggcggtc cccgccagcg cccggtcctt gaacccgggg
      301 gtggcattgc ggaacgcctc gcccgccgcc ataaagccgt cgatctccat gcgccgctgc
      361 cacatctcga ccagcgcctg gtcgcgggcg tcggtcccca tcagccgcgg ctccggctgg
      421 agcgcttcga agtagcggca gatcgccacc gattccgcga tgcaggtgcc gtcgtcgagc
      481 tcgagcatcg gcaccgtcgc cgacgggttg cgggccacgt agccgggctc gtgctgggcg
      541 cgggcgcgca ggtcgacttg ctcgaccggc acctcgatcc ccttctcggc caggaagatg
      601 cgtacccggc gcgggttcgg cgctcccgcg aagtcgtaca gcctcattgt cccctcccct
      661 atgtcgtgcc gcctatcccg cggcgcacgc ggcctctggc tgcgccccca tcgcggcctc
      721 gatcgccatc ctcagctcat catagcttcg gcacaccgga aaggccggga actcctcggc
      781 cacgttgtcg ggcgggcgga acaggatgcc ggtgtcggcg cgcgccagca tccgggtatc
      841 gttgtaggaa tcgccgatcg caacgacctt gaagttgagc gactggaacg ccgccaccgc
      901 ccgccgcttc gggtggcgct ggcgcaggcg gtagccggta atgcggccgc cggcgtcgat
      961 cgccagggta tggcagatca gcatcggccg gccgagctga tccatcagcg gcgccgccag
     1021 ctcgtagaag gtatcggaca ggatcgcgac ctggcagcgc acgcgcagcc agtcgaggaa
     1081 cgccaccgcc cccggcagcg gcgccaacgc ctcggccgcc gcgcggatgt cggcgaagcc
     1141 gaggccgtgc tcgtcgagcg ccttgagccg gtggcgcatc agcgcgtcgt agtcggccac
     1201 ctcgcgcgtg gtcaggcgca gcgcgtcgat tccggtccgc tcggcaagcc cgatccagat
     1261 ctcgggcacc agcacccctt cgaggtcgag gcaggcgaca tacatgtctc gcaagtctcc
     1321 gtcggtcgcg tcgatggctg cgccgtggca gcgcgccggt tcaggaattg ggtgcggcga
     1381 cgccgaatgg gaagccgcga ccgccggccc gtccggtcac gcggaccggc atcgctcagc
     1441 ccatgcccga cgcgccgatg cgttcggcga tcgcgaccgt gcggtacatc gagcgcagca
     1501 ccggccgctc gatcagcttg ccgtcgacca ccaccagccc ggtccggctc tgctcgaacg
     1561 cggcgatgat ccgccgcgcg cgctcgacct cctcggccct cgggctgaag gcgcggttga
     1621 tcggcgcgat ctgccgggga tggatcgccg ccttgccggt gaagccgagc gcggctgcgc
     1681 gccgggcctc ctcggccagg ccctccgggt cctcgaggtc gagaaacggc acgtcgatca
     1741 ggtcgaggcc ggcgccggcg gcggcatgga ccagacgcga gcgggcataa agcagcgact
     1801 cccaggccaa ggtcgtgcgc agctcggccg ccatgtcgac cgcgccgaac aggagcgagt
     1861 ccatgcgcgc gctcgcgcgg gcgatgtcga acgccgcgtc cagcccgtca ttggtctcga
     1921 tgatgacgtg caggcggatg tccgcgcacg ctgcgccggc gagcaggtcc gcgagcaggc
     1981 gcacctcgtc ggcgcacttg accttgggca ccatcagcgc cggcggcggc gtcggtgatt
     2041 cgaggatcgc ggagatgtcc ttgagcccgt cggcggtgcg caggctgttg atgcgcacga
     2101 tcctctcgac gccgtcgtcg gcctggggct cggcgaacaa ggccagcgtc cgcgcgcgcg
     2161 ccgcgtcctt gtcgtgcggc gcgatcgcgt cctcgatatc gacgcaaacc atgtcggcgc
     2221 cggtggccag cgccttcggg tacatcgcgg gcttgtcgcc gggcgcgaag atcaggctgc
     2281 ggcgcgcacg cacgggtcgg tcggacatgg cggttccgag gcttcaggga gcggcaacgg
     2341 cgtggatgtg gctgccgccg tggcgggcga gcagcgcgct cgccgtcgcc gcgcgctcgc
     2401 gctcgcggac gcgcacccag accaccagcc cgccgtcggc gaggttgcgt tggaaccgtt
     2461 cgctgtagcg gtcctcgacg aacttcgaca gcacgccctc taccgtgctc tcgaccgccg
     2521 cgccggtcgt ggtggcgacg cccaccgggc cgccgaccgc ataggccatg ccggcggcgc
     2581 cgattgcgcc gatcgccgac agcccttcgg cgatggcccg cgccgcggct tcgaccgcgg
     2641 tctcgggcgc caggtcctcg cgcggggtgt cgggcaggtc ggcgagctgg tcggggctcg
     2701 ggatgcggtc gtcgaaatga tcggcgagcg cgtcgtgcgg cgccaggatg ctcacgtcgg
     2761 cggcgtcgaa gccggcgccc agcagtgacc gcagcacgcc gcggaacgcg ccgacgtcat
     2821 cgaagatcgc ggcacacaga tacgggaagt ccggctcgtc gaccatcggc gctccgcctc
     2881 gattgcaggc cgcggccagt tgacatcagc gcgctctcgc gggcaacagc ctcgtgtcgg
     2941 catgaactcg cgcggcaact ggcacgcggt cggcgatctc cgatcacggc cgaggcaccg
     3001 cgtcgtcctc gatctcctcg accggctgca tcgagcccgc gtcgatgatc agggtgcgcg
     3061 gcggcgccgt cgatgcgccg gacgcgccgc ccggaatgaa cgacacccgg aacgcgggat
     3121 tgccgtggtg catgatcggc gtcagctcga ccacgctgcc gtcgaactcc gccttgatcc
     3181 attgctcgat catccacgtc cgcccgacca tgtgcacgaa ggttgcggac gccggcaacg
     3241 ccgaaagaca agcccccgca agcagcgtgc ccgcggcggc gaggcgaagg atcgacatgg
     3301 tcacgtccac ggttcccccc ggctcggacc gggcgcgatc ggtgccacgg gcgggcggcg
     3361 cggcgaaggg gcgcactacg gggccgtcgc ccggacgcgg cggaccagct cccaggtctg
     3421 ccaagccagg tagccgagcg cgcccagcgc gtaggcgagg aagaagacgt caaaccagcc
     3481 cagccacgcg aacggcccga tcagataaat gaaatcctcg atctcgaagc cgtggaaata
     3541 ggggtgggcg actgcctcgc tgccgtggcg ggtctcgttg gcgagccgca ccgccagcac
     3601 ggccgggttg gacagggcgg cggcgagtcc cagcgtcagc gccgcgctgc ccagctcgcc
     3661 cgcggcgagc ccgatgccgg cgcccagata catcatcgtg tagttggcgg cgccgacggc
     3721 atagtcgaga taatggccga accgcgaggt gcgcctggtc gctcgtgcca gctcgccgtc
     3781 gagatggtcg atgaacaccg ccagcatgaa cagcccggcg ccgagaccga agcggccttg
     3841 cgcgagcgtc cagccggcga gcgcgccaag cgcgaaggac aggccggtca ggtggttggg
     3901 cgcgatcggg gtccgcgcca gcgtctgcac cagaagcgcg ccgaggcgtt gatcgtaggg
     3961 cacgcccctg gctccgggtt tcaagtccgc catcgcgcgg ctccgcagat cagcgcagat
     4021 agccgtggtc gcggaaccag gcgaccgcgt cgctcagcgc ctgtgccgcc ggccgcggcc
     4081 ggtagccgag ctcgtcccgt gcccgccggc tcgagtagta catgggcttg cgcgccatgc
     4141 gcgcgccgtc gaccgtggtc atcggctcgc gcccggtcac gcgcgccgcg gcctcggcca
     4201 gataggcgac cggcagcacc gcggcgtgcg gcagccgcag ccgcggcggc cgccggccga
     4261 ccagcagcgc gacctcggcc aggatgtcgc ggagagtcat gtcctcgccg ccgaggatgt
     4321 agcggcggcc cgggaggccg cgctcgaagg ccagccagtg cccttccgcc acgtcgtcga
     4381 catggaccac gttcaggccg gtctcgacat aggccgggat gcgcccggtg gcggcttcaa
     4441 cgaccatccg cccggtcggg gtggggcgga tgtcgcgcgg gccgatcggg gtcgaggggt
     4501 tgacgatcac cgccggcagg ccctcgctgc gggcaagctc gcgcacggcc gcctcggcca
     4561 ggaacttcga ccgcttgtag ggcccgatca tgtcgtcgag cgtcgccggc tgctcctcgt
     4621 cggcggcgct gccgtccgcc ttggcgccga gcgcggcgac gctcgaggta tagacgatgc
     4681 gcttgacccc ggcgtccgcc gccgcccgca tcagggcgcg cgtgccctcg atgttgacct
     4741 tcatcatcgc cgaggggtcg cgacaccaga tgcggtagtc ggcggcgacg tggaacagcg
     4801 cctcgcagcc gcgcaccgcc gccgccagcg atggcggatc ggtgaggtcg ccctcgacca
     4861 gctcgacctc gagaccgtcc aggttgcgcc ggtcgccgga tgcgcgcacc agggcgcgca
     4921 acgccatgcc gcgggcgagc aggcaacggg cgagcgccga gccgacgaag ccggtcgcgc
     4981 cggtgatcag cgcggtcatg cgctcacgtc ctggcggtca tgacgcgcca cacgtcgccg
     5041 cgcggggcca aacggtgtaa tcttcccttg tgccgggatc ggcaagatcg gatgagcaac
     5101 cgccatgacc cacaccaatg tcaccaggct tgaggaactg cgccgcgaaa ccctggatac
     5161 cggcgttcaa gatgagcgca cgttgcgcgt gcagcttgcc gccgcgtacc ggattttcga
     5221 ctacttcggc tggccgcagc tcattttcgg ccacatcacg gtgcgcctgc ccggtccgga
     5281 ccggcacttc ctgatcaacc cgttcgggct gctataccac gaggtcacag catccaatct
     5341 ggtcaagatc gacctcgatg gcaacatcgt cggcagcggc gagtacccgg tcaacccggc
     5401 cggtttcgtg atccacagcg cgattcacgg cgcgcgcgag gacgcccatt gcgtgatgca
     5461 cacccacacc cgtgcaggga tggcgatcgc ggccctgaag gatgggctaa attgctcaga
     5521 ttttgctgga atttctctct ataaccgcgt tgcgtaccat gatttcgaag gcctgaccgt
     5581 ccgcatggac gaacgcgagc gcctggtccg gagcctggcc gacaagaacg tcctggtgtt
     5641 gcgcaaccac ggcctgttga cctgtggcaa gaccatcgcg gaggcgctct tgcgcatgtg
     5701 cacccttcag accgcgtgcg aggtccagct gaccgcccgc gccgccggcg gcgccctggt
     5761 cgagccctcg cccgaggtct gcgagcggca cgccgccagc ttcgattcgg gtaatcagag
     5821 cgcgcgcgta ttcgccgccc tgatgcggct gatggacgag cgcgatccga gctatcgcaa
     5881 ctagcgtccg ccggcgcaat cccggtcgtc tgggtatccg cccggttact tagggcggag
     5941 ggggacggcg ccgcagccgc tccccggttc ttgtcgcgtc gcggttttgc catagatttc
     6001 acagggatgg gcgcgagcga caagtcccgc gtcggcgcct cgttggtgct ggccggccgg
     6061 aagacccgct gagatgtcgc agatcgcggt caccgtatcg tcccgatctt cgcccggtta
     6121 ccggttcgat cgcctgtcgg tgatggtggt cgacgacaac cgccacatgc tcaacctgat
     6181 cgcggaaatc ctgcgcgggc tgtcgatccg ccgcctgatc aggctcaccg acccggcgga
     6241 cgccttcaag gagctgctga tcgcgccggt cgacatcatc gtcgtcaacc acgtcatgga
     6301 gccgttgacc ggcatcgagt tcgccaagct ggtgcgcttc ggcgacgaca gccccgacct
     6361 gttcgtgccg atcatcatgg tcaccggcta cagcgacgcg gcgacggtgc gggcggcgcg
     6421 cgacgccggg gtgaacgagt tcctggccaa gccgatctcg gtccgcggcc tgtacacgcg
     6481 catcctcgag gtcatcgacc acccgcgccc gttcatccgc tgccccgact tcttcggccc
     6541 cgaccgccgc cgccaggtgg tgccgttcga tggccaggac cgacgcgtca gcgcgccgag
     6601 cgaatcgccc gaaggcggcg cggcgcaact gattccggtc gccgcgcgca cgtgaccagg
     6661 taccgggacc caagccgatg accaatcgcg accggccagg ggatgacgcc accgcggcgg
     6721 ccctcattcg gatgccgaac aagatcaagg acaaggtcac cctcggcggg cggatcaaag
     6781 gagacggttt cgaccccaag ttgctgcaac gggccgagcg ccgggtcgag gcgatgcggg
     6841 acgtgtttct ggacgccgcg cgggacgaca tcgaccggct ggtcgccgca ttgtccaacg
     6901 cccagaccga tgccgcccgg cgcggtcgcc atctcgacac gcttgagaag atcgcgcacg
     6961 agatcaaggg ttacggcagc cacatcggct acgacctgtt gacccggttc gccgattcgc
     7021 tgtcgctgtt cctgcgcaag gcgaagacga cggttgccgt gaagctcgag gtggcggagg
     7081 tccatgtcga cgcgatgcgc ctcgtttacc atgacgggct gaccggcgac ggcggcgagc
     7141 agggcgcgga tttgaccgcc ggcctgcgca gcgcggtcgg cagatacctt cgcgccgagg
     7201 acttctgaac cccgcggccg gcggccgctc ggcgagccgg agccgtcctg cgggtggtgt
     7261 ctcagtttga aatccgcgac ccctgacgcg agtgtcgcgt cctgcctttg gcttctatag
     7321 gcttagcgga agcacgggag gctggccttg cgcgatacca ctcacgcacc tgagctgaag
     7381 acagaccgcc ttatcgcgag cggctttgag ttatcgggac ggtgggagtt ggacaccgct
     7441 tcacgcgcac gattcatcgg cgaagctccg aagcaaccgg gggtctacgc ttttgcggtg
     7501 gacggaaagg ttctttatat cggttccgcc cagcgtggca tcgccaaacg gttgcgtcgc
     7561 tacgaaatca ccgaaatccg gctgacggcg tttcgcgtca gagctttgat aaccgaagcg
     7621 ctcggggacg gttccgaggt tgcagtgctc acgattgtcc catgctcaat gcgatggaag
     7681 ggccttcctg tggacctgat cgccggactt gaagagggcc tcattcgcga gttgcgacca
     7741 gaatggaata ttcgcggact cggcgctctg cgaaagaaaa cggcttccga aatttcaaac
     7801 tgagacacta ccgtcctgcg cgcccgcttg acccgccggc gtgatgatga ttggctgatg
     7861 ttcttattct gttccaatgg catatggcct cgcggacgac atcgcgcggt gcggcgcccg
     7921 tcaccctcgg cgagcttgcg gccgagggcc gtcgcgtgtg gctgtggtgc gagcgctgcg
     7981 cgcgcagccg cagcatcgcc gccaccgtgc ttgcccgtcg cctgcccgcc gatttgcccg
     8041 tgccgctgat cggccgccgc gtccgctgcc gcatctgcgg cgggcgcgaa gtgcacagcc
     8101 gtccgcactg gccgcctggc gagacggtgg cctcgcatcg ctggcacgac ggcggctcgg
     8161 gagccgagga atgacgcgcg tgggcgcacg gggcgggcag cggaatcgcc ctaaataccc
     8221 ctgtgcagaa gcaacagccc ggcacccacg cgggcgatgt cggtcaaaaa aaagcgcgcc
     8281 gaatgaacaa agcgaccact gcgacccgaa cccctggcct cggggtcgac gaggctgaac
     8341 gggccgagct ccaccgctac atccgccaca tcgaccgcgc gctcaaccgg ctgtggaacc
     8401 tggggcgcaa cctggtggtc ggctggcacc cggccgaggc cggcctcagg gtcctggtca
     8461 tcccgcatca catgctgcgc gagctcgccg agcaggacgg cgacctgctg tgcgacggcc
     8521 atcgcgtgac ccatgcgcgc ctcgacgcgc tcggccgcca gctcggcatc gagccgatcc
     8581 aggtgcgcct tccgaaccgc cccgggcggc ccggcatcgc ccacaaggcg atcgaatcgg
     8641 tggtgcgccg ctacagcgtc accaagtcga accaccgcgc cgcgatcctg ctcgacatcg
     8701 tcggcttctc gctctactcg ccgctcgagc aggtcaccct gctcaacagc ctcgcgtttt
     8761 cgatcaacgt cgcccagagc cgcgcgctcg ccaacaagct gcgcatcgac gtgagccgca
     8821 gcaccaccgg cgacggcttc tatgtctgga atcgcgacga gggcctcgag gccgacgtca
     8881 acctgttcta cctgatgatg ctggtgctgg ccgacaacgc gctcgcgcgg cacaagggcg
     8941 cgcccagcgc agtgccggtg ctgcgctcgt gcttccatct cggcagccac tacgagtact
     9001 acccggccga cgggctcaac ccgtcggtca ccggcttcat cgtcggtgat ctcaccatcg
     9061 agctcgcgcg catggtcggc aaggcgttgc ccgggcaggt gctgatcggc cagttcacgc
     9121 gcccgaaagc cggcgtgggc ggacacgcgg gggccctgtc gacgctggct ttcgtcagtc
     9181 gcgcccaaga ccgcctcgac cagctgcacg cactcgagct ttccggccag cgcatcacgg
     9241 cgatcaagtg ttaccttacc ggcaagcgca tcggcggcgg tgtgtacagc atcaccaaat
     9301 acgccctcag cgacaagcac ggccggcaaa ggaacgtgtt caacgccaag gtgaacatct
     9361 atcgaggcga tgccgacgtg ctctatttgg gcctggagaa caaggacctg gaacgtttcg
     9421 atgccgaagc tgccgagccg cgcacgcgcg ccagacccgg tgtccgggcg agcgtcgact
     9481 aggccgcgcg ggcgcggcgc cgcgtgacca cgctcgccgc ggccgagacc gcctgccgcg
     9541 cagcgctggc ggcggacccg aacgacgccg atgcgctcga ccagctcgcc cgcatccagc
     9601 tggagcgcgc gcccgagcag gcattatcgc acttgctgca cgcgcatgcg gcgcgcgtcg
     9661 gcggtcgtca cgccgaggcg gtgcgggccg cacgcgaggc gctgcggcgc gcgcccgcgg
     9721 acgtcgaagc gctcaacctg ctcgggctca gcctgcgcga cctcggcgat ctcgaccagg
     9781 cggtcgcggt ctggcagcgc gccgtccgca ccgatcccgc gctcgccgac ctgtggctca
     9841 acctgggcaa ggcgctgtgc gaggccggac gggcggagga cgccttggcg cctctcgccg
     9901 aggcgctgag ccggcggccc gaccatggcg acagcctgct cacgctgggc aacgcgctga
     9961 tcgcgctcga gcgcgcgccc gaggcggcgg cggcgttcga gtgcggcctc gccggcgatc
    10021 cgcgctcggc ccggctgcac gccaacctcg ccctcgctcg ccgccaatgc ggcgacctcg
    10081 acggtgccga gcatcacctg cgccaggccg tcgcgctcgc cccggcgctg gccgaggcgc
    10141 acaagaacct ggccatgctc gacctgctgc gcgggcgctt cgccgacggc ttcgaccgct
    10201 tcgcctggcg ctggcgcgag cagcgcgcgt tcaaccgccc gcgcccgctg ccgcagccgc
    10261 cctgggacgg cagcgccctt ggcgggcgga ccatcctggt gtggggcgag cagggcgtcg
    10321 gcgacgagat catgttcgcc tcggcttacc ccgaggtgat cgcggcggcg gcgcgctgcg
    10381 tggtcgagtg cgaggcgcgc ctggtgccgc tgttccagcg ttcctttacc ggcgcacgca
    10441 tcgtcgcccg caccgagccg ccggccgcgg agctcgccga ccccgcgatc gacctgcaat
    10501 gcgccgcagg cgatctgtgc cgctggctca ggcggtcgcg cgaggacttt ccggtgcccg
    10561 cgccttacct gcttgccgac cctgggcgaa tcgcggcggc ggtggcacgc gtgcgggccc
    10621 tcggcgacgg ccccaaggtc gggctcgcct ggcgcagccg cacgccggtg tggggcgcgg
    10681 cgaaaagtgc cccgctcgcg ctttgggatc cgatcctgtc ggtgccgggg ccggtgtgga
    10741 tcgacctgca atatggcgat tgcgccgacg agcgcgcggc ggcccgcgcc cgcctcggcg
    10801 tgacagtcca tcacgacccc tcggtcgacc agatggcgag cctcgacgac ttcgccgccc
    10861 aggtcgccgc cctcgacctg gtgatcacca cctccaacac caccgcccac atggccggcg
    10921 cgctgggggt agagacctgg ctgctgctgc cccacatgcc cgactggcgc tggcaggcga
    10981 cgggcgagga ctcgctctgg tatccgcgca cgcgcctgtt ccgccagccc tcgcccggcg
    11041 actggcgaac cccgatcgag tcggtggcgg cggcgctcag gggccggctt tgacctcgat
    11101 caaggacagc gccccgccgc cggcgtgatc tggcgggcga ccgccatgac cgacctggtc
    11161 gcaaaatacg ggctggcgcg ggtgccgcgc tacaccagct atccgaccgc gccccacttc
    11221 gattcgcgga tcgggccggc cgcgcaccac gactggctcg gcgcgctcga cgccggcacg
    11281 cgcgtgtcgc tctacctgca tgtgccattt tgcgcgcgcc tgtgctggta ctgcggctgc
    11341 cacaccacca tcacccgcga tcccgagcgg gttcaccgct acgccgacct gctcgcgcgc
    11401 gagctcgacc tggtgcgcgc ggcccttccc gcccggctcg cggccggcca cgtccattgg
    11461 ggcggcggca cgccgaccat gctcggcgcc ggcggcttca cccggatcgc cgcgctggta
    11521 cgcgaccgct tcgcgctcac tgccgacgcg gagatagcga tcgagatcga cccgcgcagc
    11581 ctcgacgccg agaccgcgcg cgcgcttgcc ggcgccggcg tcacgcgcgc gagcctcggc
    11641 gtgcaggact tcacctcccg cgtccagcag gccgtcaacc gggtgcagcc gttcgagcag
    11701 gtggcgcggg cggcagcgtg cctgcgcgcg cacggcatcc gtgcgctcaa cttcgacctc
    11761 atgtacggcc tgccgcacca gaccgccgcc gacgtcggcc gcagcgtcga cctcgccgtg
    11821 cgcctggccc cggaccgtgt cgcggtgttc ggttatgcgc atgtgccctg gctcaagcgc
    11881 catcagcggc tgctcgatga ggcggcgctg cccggtgccg ccgcacgccg cgtccaggcc
    11941 gagaccgcgg cagcgcgcct cattgcccac ggctatgtcg cgatcgggct ggaccatttc
    12001 gcccgccccg gcgacgcgct tgccagggcg gcggccgaag gccggctccg gcgcaacttc
    12061 cagggctaca ccaccgacga cgctccggcg ctgatcgggc tcggggcgtc ggcaatctcg
    12121 accctgcccc agggctatgc gcagaacgcg ggcgacctgc ggcaatatcg cgggcggatt
    12181 gccgccggcc ggttggcgac ggcgcgcggg gtcgcgctcg acgacgacga ccggatcagg
    12241 cggcggatca tcgcccggct gatgtgcggc ttcgaggccg acctcggacc cgccgacggc
    12301 gcgctggagg tcgatcgcgg aacgctcgcc gagatggcgc gcgacggtct ggtggtcggc
    12361 gacggctcgc ggctgcgcgt aaccgaggcc ggccggccgt atgtgcgcac ggtcgccgcg
    12421 tgcttcgacc gttatctgtc gtcgcgcgca gggcgccacg cacaggccgt ctagcaggtc
    12481 gcggccgaaa accgaaattg gctgccgaga aactgtctat acgatcgaat taaattaaaa
    12541 tctcaaccgg cacgaagcgc ctacggcgtc ccggtgacgg tcgcgatcag cggtgccgac
    12601 ttcatctcca ggtgcccctc ctggtaaacg ctgtagcgga acacgccgtt gatcacgtgc
    12661 gtgcccgggt aggtcggcgt gaccgcgacc gagaacacga ccttgccgtc gtccatcttg
    12721 ccggcaacga cctcgtcctc gaacgcgacg ccgccgtcgg tcgatagctt gatgatccgg
    12781 tggcgataga cgtcggtcag cccaaaaccc tcgcgcgggg tcaacgtcgc ccgcaccgcc
    12841 gtcgtctcgc ccaccttggc ggtcgcgttc tcgaccgtga tcgcgaccgg atcctcgaac
    12901 gcctgcgcgg cggcggccgt tgtgccggca agcccggcgg cgaccgcggc gagcgcggcc
    12961 aaggcgacgc gccgcccgcg gcgcacgaac cctccgaccg tgccactgcc catatctcgc
    13021 tctcccatcg tcgccgagga tcgcccgcgc cgcttatcaa gaagatcggg ccagccgcgc
    13081 aactggcggc tggcccggaa acggactttg gtccgcgact ttgccgctca gcgggccggc
    13141 caccgaaccg gcagccggcc cacacagggg tcttcgtccc ttactcgccg agcgcgaacg
    13201 cccagatgac gccgccttgc ggcaccacgg tgtcggtgcc gagcaggttg ttcatcaggc
    13261 cctgctggaa cgccgggtcg acgccccagc cggacgccac ggcgacgtac tggacgccgt
    13321 caacctcgta ggaggccggc ggcgccatga tgcccgagtt ggtgcggaag ccccacagcg
    13381 cctcgccggt cctggcgtcg tacgcccgga agtaccggtc gttggtgccg ccgttgaaca
    13441 ccaggccacc gccggtggtg agcaacgagc cccagttcat catgccgggg tacagctggc
    13501 tccagacctg ctcgccagtg ttgaggttcc acgcctggat ctcgccgaag tgccgggcac
    13561 tcttctcgac cacgaagccg atgtccggaa tgtcgacacc ggtccaccac tggccggcca
    13621 cgtattcctg gaccttgcct tccaggaagc cgcaatggtt ctcgttggcg gggatgtaga
    13681 gcaggccggt cttcgggttg taggcctcga acggccagtc cttgccgccc cacaggctgg
    13741 ggcagaagcc ggcgtacttg ccggtgccgg gcttgcggtc atcgttgtag gtcggccgcc
    13801 cggtgctcgg gtcgatgctc ttgaacacgt cctgcttgac gtaggccttg gcgtcgacga
    13861 agccgatgcc gttggccttg cgctcgagcc agtacaggta gccgttgcgg gccggcttga
    13921 tcaggccctt caccattttc ccgccgtgct cgaaattgac caccgtcggc gcgttcatct
    13981 cgtcccagtc ccacgagtcg ttccagtggt actggaagtg acccttgatc gagccgtcct
    14041 tcggatcgat cgcgatggtc gacgaggtgt agaggttgtc accctcgcgc tgatcgccga
    14101 accacggcga gccgttgccg gcgccccaaa aggtgaggcc gagctcggga tcgtaggtgc
    14161 cggtgttcca taccgcaccg ccgccgcgct tccacgtatc gcctggccag gtgtcgttgc
    14221 cgggctcgcc cgggccggga atggtgaagg tcttccaggc cggcttgccg gtctcggcgt
    14281 cgagcgcctg gacgaagccg cggacgccga actcgccgcc cgagaccccg accatgacct
    14341 tgccgtcgac gatcagcggc gccatggtct ggtagtagcc agtgtggtag tcctcgacca
    14401 ccgtctccca gaccaccttg cctgtcttgg cgtcgagcgc gagcgtggtg ggctcgaggc
    14461 cggtgaggta gacgttgttg ccgtaaagcg cgacgccacg gctggtgttg tgcaatgcgc
    14521 cgaagtcgtc cggcagctcg cgccgatagc gccagatcag atcgccggtc gccgccttga
    14581 gggcgatgac ctgggcatag ggcgtggcaa cgaacatgac gccgtcattg acgatcggcg
    14641 gcgcctcgtg gcccgagtcg accccggtcg agaacgacca taccggcttc aggctgccgg
    14701 catttgcggt gttgatctga tccagcggac tgtacatcca gccccagtaa ttgccgcgcg
    14761 tcatcagcca gttcccggct tcgggattgg tcagtctctg gctggtgacg gtgctgtagt
    14821 cggacagcat gcctgcggcc gcgaccgcag gcatcaggcc gacaccgagc agcgcagcgt
    14881 atttagctac tttcattgcg gatcctccct ctgtcgtacg aagcgagcgc ggcacgaagc
    14941 gtggacgaag cccaactcca cctcatgccg gcttcttgaa gccgacggca ccttcgaact
    15001 cgccggcggc gacgatgacg ccatcctcca cctttaccgg caggatggcc agtcgtcgcg
    15061 gcgctggccc ggcgacgacc tcggccatgt cgcccggatt gtactcactg ccatggcagg
    15121 agcaatagag agtgtcgttc tcgggttgcc acatcgacac cgggcaaccc tgatgcgtgc
    15181 ataccgccga gtagacgacg acaccctcgg ccgcaaaccg gcgggtccgg tcggtgatcg
    15241 ccgcgtcggc cagccggatc agggcgacct ggttgagccg cgagcccttg cgcaccgtcc
    15301 cggtggcggg atcgatcggg taggcaattt cctgggggcc gccgagcggc aggtcgtcga
    15361 ccttgatgac ctcgcccttc ttgtcgccct tcaggaacac gaaccggtcg ccgggctggg
    15421 gacgcatttt ctcagggttg tcggcggcgc gcaccgcggc gagtttcagg aacggtagcg
    15481 cggcacccaa gccaagaccc gacctgagca acgcccgtcg tgcttttcca aacgcttgca
    15541 aggggttatc tccagtccag ccgtcggtcc tctcgccccc cgatagataa catctcggtg
    15601 ccgccgttgc caggggcggg cggcgttcac ggcgccagcg cgaacgcgat caccgcatcg
    15661 ccctgcggga acccgaacag gtgatggccg ccggctgcga ccgcgacgta ctggatgccg
    15721 tcgacctgat aggtaaccgg cggcgcgttg accccggcgc cgcactggaa ccgccacagc
    15781 cgcgcgccgg tggccgagtc ataggcgttg agatagccgt tgccctcgcc catgaacacc
    15841 aggtcgccgg cggtcgccag cacgccgccg agcagcacat cgtcgctctt gacctgccag
    15901 cggatgcggc cggcgtcggc gaggtcgatg gcgctgagcg tgccccagcg cggctcgttc
    15961 gcgacctcga gcaccgaata gcgccgcgcc ggcttgtccg cagtcgccgg ccgctcgcgc
    16021 acgctgtagg tggtcggcat gtgcaacgcc gccagatagg cgagcccggt gcgcggatcg
    16081 aacgaggccg gcgaccacga cgcgccgccg cccgcgcccg gcgcgatggt cacgccttcg
    16141 ccggtcgggc gccggaacat gttgctttgc gggacgaacg gttccgactt gaacaacagc
    16201 tcgccggtcg cgcggtcgag cacgtacatc cacccggtct tggacgccga ggccaccgcc
    16261 ttgaccgtgc cgccgtcgcg cgcgaggtcg accagcaccg gcggcgtgcc gacgtcgtag
    16321 ccccacaggt cgtgcggcac ctgctgatag aaccagcgca gctcgccgct gcgcgcgtcg
    16381 agcgccacca gcgccaccgt gtacaggttg tcgcccgggc gcgagacgtc gtccatctgc
    16441 ggcgccggat tgccggtgcc gagatagagc agcccgagct cgggatcgag cgacggcgtc
    16501 gaccaggtcg agccgccgcc gacctgccac gcatccgcgt actccgcctg gcgcgcccgc
    16561 tcggcggcga tgtcacgttc gagcgccacc ccgtcgggcg tgtgcgtgcg ccactcccct
    16621 tcccagccgt cggccttggt ggtgtaccag cgccacgcct cggcgccggt cttcgcgtcg
    16681 aaggcggcga gatagccgcg ccggccgaac tgtccctcga agccgatcac cgcgccgagc
    16741 tggttgccct cggcgtcatc gacatgcagg ccgtagcccg cgccggtgac tcccgcgatc
    16801 accaggccgt cgaacaccag cggcgccatg ttggcgccga gcccgctggc gccggtcacg
    16861 cgctggccgc gcagcgggtc gtcggcggca agctcggaca aggtctcggt cggcctggtg
    16921 tcgggggcgg cgatggtaac gtcccatttg accgcgccgg tggcctggtc gagcgccacc
    16981 agccggccgt cggcggtggc catgtagacc gcgccgtagc cgagcgccgc gccgcggttg
    17041 gccggcccgc agcacagctt gtcggtgacc atctggtggc ggtagcgcca gacttgctcg
    17101 ccgctggcgg cgtcgagcgc gaccacgtcg ttgcgcggcg tggtcaggta catgatgccg
    17161 tcggcgacca cgggcgtggc ctggaacgtg gcgctgatcc cggtctggta gatccagcgc
    17221 ggcaccagcc tcgccaccga cgtgcggtcg atctggtcaa ggctcgagaa ccgatggtgg
    17281 gcgtagccgt ggccatagga cagccagtcg ccgctggccg gcgactcggc ccgcagccgg
    17341 gcatcgtcga ccgggcccgc cgccgccgcc gcggccagcg acacgccaag cagcgccccg
    17401 gcaagactcg tcttatgatg agatttcatt gtcctttccc gcctccctcg gcaacggcgt
    17461 ccccgaacac cgatcttgaa agggcgaggc aggcaagtct actgcggccg cccgaccacc
    17521 gcgcgccggc tcttgcgccg gtgcgtggcg gtcgccaagt ctagcagttc gcaggcaccg
    17581 tgaacggatg aggtcgaagc gatgacatcg gcaaccggga cgacccgccc ggcggacgcg
    17641 accgcgcgcc tgcgcggcgt tcgccgcacc gtcaccggca tgcccgcctc cgacggcgcc
    17701 ggagtgcggc tgagccggat catcggccag ccggggctcg acatggtcga tccgttcctg
    17761 ctcctcgacg agttcaagtc cgaccggccc gaggactaca tcgccggctt cccggagcat
    17821 ccgcatcgcg gcttcgagac cgtcacctac atgatcgccg ggcgcgtccg ccacgccgac
    17881 aacaagggcc atgccggcgt catcgaggcc ggcggcgtgc agtggatgac cgccgggcgc
    17941 ggcatcgtcc attcggagat gcccgagcag gaggacggcc tgctgtgggg cttccagctg
    18001 tgggtcaacc tgcccgccgc cgacaagatg aagccgcccg cctaccagga gttccccgcc
    18061 gagcgcattc ccgcggagag ccgcgacggc ggcgtggtcg tgcgcgtgat cgccggtacc
    18121 accgcgcccg gcaccaccgg gcccgtgacc ggcatcccga ccgcgccggc ctatctcgac
    18181 gtggcgctcg ccgccggcag ccgtttcgag gagccgctgc ccgcggcatc gagcgcgttc
    18241 gtctatccgt tccagggcaa ggtccgcgtc gccggcaacg gcgcggccac cgagcttgcc
    18301 gcgggcacgc tcggcctgct cgggcccggc gacgtggtgc gcatcgaggc cggcgacgac
    18361 gacgcccgcc tgctgctcgt cgccggccgc ccgctcggcg agccggtcgc ccgttacggc
    18421 ccgttcgtga tgtcgaccaa ggccgagctg acgcgggcct accaggactt ccaggccggc
    18481 cgcttctgag cgcggcttgc ccgcgcctca gcgacccttg aacactggcg cgcgcttgtc
    18541 gcggaacgcg gtcatgccct cgatccgatc ctcggtcgcg aacgcgcgca gcaccgcctg
    18601 gcgctcgaag gcaagccctg ccggcagccc ggtggcgtag gccgcaagca ccgcctgctt
    18661 cgccagggcc agcgccaccg gcggcaggcg ggcgagccgc tcggcgagtg cacgtgcctc
    18721 ggcgagcgcc gtgccgggtg cgaccagctt cgagaccagc ccggcacgga gcgcgtcctc
    18781 ggcgccgatc ggctcgccgg tcagcatcat ccacatcgcc agcggcttgc ccgcgatccg
    18841 cgtcacccgc tgggtgccgc cgtcgccggg cagcacgccg atcttgatct cgggctggcc
    18901 gaagcgcgcg tcggcggcgg cgatgatgat gtcgcagacc agcatcagct cgtggccgcc
    18961 gccgaaggcg tagccctcga ccgcggcgat cagcggcttg gcgaagcgct cgatcgccgc
    19021 ccagtcggcg ttgcgccgcc cgtcgtcgag cacctcgggc ccgccggcgt cggtgaactc
    19081 cttgatgtcg gcgccggccg agaacacttt cgcgccgccg gtcagcaccg cgcacttgac
    19141 cgccgcgtcg gcgtccgccc aggccagctc ctcggccagg cggtggatga cggcgcggct
    19201 cagagcgttg cgcgcctcgg gccggttgag ggtgagctgc agcaccccgt ccgccggccg
    19261 cgcgctcagt acggtgtcgg tcatctcgcg tccctggtga gtcgcgggcg ccgcccgcgg
    19321 gctcggcctt gtactgcgat gccgccggcg ccgtccacag ccggcggttt ttcgccggcc
    19381 ggcttttgcg ctataccagc gcccgcagcg tggcgcggag ggagggccga ccatgaacga
    19441 gtcgcaaccg aagctcggtt acatcggcat cggcctgatg gggacgccga tggccaggcg
    19501 cctgatcgag gccggctatg acgtgaccgt ctggggccgc agcccggcca agatcgagcc
    19561 ggtgctggcg gcgggcgcgc accgtgccga cgacgccgcc ggcgtcgcgc gcgccgccga
    19621 catcgtgctc acctgcctgt cggataccgc ggcagtcgac gcggtggtgt tcggcaaagg
    19681 cggcatcgcc gccggcgccg gccccggcaa ggtgctggtc gacatgtcct cgatccgtcc
    19741 cgacgcggcg cgcgagatgg cagcgaggct caaggccgag accggcatgg gctggatcga
    19801 cgccccggtc tcgggcggag tcgcgggatc ggagtcgggc aacctcacgg tcatggcggg
    19861 cggcgcgcaa caggacttcg accgggtcgc gccggtggtc gcgcacctcg ccaagcgctt
    19921 caccttgatg ggccccaacg gcgccggcca ggcgaccaag ctgatcaacc aggtgctggt
    19981 ggggtgcacg gtcgcggtgt tgtcggaggc ggcgagcctt gcgctgcggg cgggaatcga
    20041 cgccgagcgg attccgcagg cgctggccgg cgggcgcgct gattccttgc cgctgcaaca
    20101 gttcttcccg aagatggtca agggcgactt cttcgtcgag tcccacctgc gcaccatgct
    20161 caaggacctc gagacggtgc aggcgctggc gcgcgagact acgactgcga tgccgatgac
    20221 cggcatggcg accgagctgc atcggctgat gatccagcgc ggcttcgccg accgcgacgg
    20281 caccgcgctc gccatgctct accgcgccga ccccatctga gccaggcggc gagcggcaac
    20341 atggacatcc tcgacggcat ccgggtgctc tcgttcaacc acttcctgct cgggccggtg
    20401 ggcgtgcagg tgctcggcga cctcggcgcc gacgtgatcg cgatcgagcc ggtcgagggc
    20461 gcgttccagc gccgctgggg cggcgccaac cggcgcgtcg acgacgagac cgtgctgttc
    20521 ctgtgcgcga gccgcaacaa gcggagcctg gccatcgacc tcaaggcgcc ggcagcgaag
    20581 gagatcgtcg agcggctcat cgccggcgcc gacgtgctgt gcgagaactt ccgcccgggc
    20641 gtgatggaca agctcggctt cggctacgag cgcgcccgcg cgatcacgcc ctcgctcgtc
    20701 tacgcctcgg cctcgggtta cggccaggac gggccctacc gcgaccgccc ggggcaggac
    20761 ctgatcgtcc aggcgatgtc ggggctgatg aacatcaacg gcagcgtcga ccagccgccg
    20821 atcccgatcg gggtctcggc cgccgaccat cacggcgcgc aggtgctcgc catgggcatc
    20881 ctggcggcgc tggtccggcg cgcgcgcacc ggccgcggct gccgggtcga tgtgaacctc
    20941 ttatccgccg ccctcgattt acagatggag tcgctggtcg ccttcgccaa cggcgcgccg
    21001 gtctcgcagc gcccgccggc gcatatcgcc ggctggtact acccggcccc gtacggcgtc
    21061 taccgcaccg ccgacggcca tatcgcgatc tcgctggcgt cgatgaccac gctcgccgac
    21121 gcgctcgaaa tgcccgcgct cgccgaggtc tccgacgacg agaccttcac ccgcaacggc
    21181 gacatcgcgt cgctgataca ggaacgggtc gcgggggtcg cctgcgccga ggtcgaagcg
    21241 atgctcgagc gccacaagct gtggtacgcg cgggtcaacg actacaccgc ggtattgaac
    21301 gacccccagg tcaggcacaa cggctcggtc atcaccaccg acaacgccca cggcacgccg
    21361 gtcaggctgc tcgcccatcc ggtgcgctac gacggcgagg tgccgccggt gcgcatcccg
    21421 ccgcagccct tgggcgccca gaccgccgac atcctggccg agctcggcta cgcgcgcgac
    21481 gatatcgacc gcctcgccgc ggccggcgtg gtgcgggtcg ccggccgcgc ataggagcga
    21541 caccggcgag cgcgctgacg cttgacgccg ccagcgggtc tgtcgccggg gcggacttgg
    21601 cgttaagatg gcgggcgaaa agagatcgaa ccgcggaggc cggccatgcg gcgctttccc
    21661 gacttcgagc ccaagggcgt gatcccggcg accctgatgg cgttcgacga cgactttgga
    21721 atcgacgagg ccggcgcgcg caagcatttg cgcgacgtcg ccgccacccc ggggctcgcc
    21781 gcggtcacgg tcaacggcca cgcttccgag gtccacgcct gcacgttcga cgagcagcaa
    21841 cgcatcctcg agttctcgct tgacgaggtc ggcgaccggc ttcccgtgat caacggcgtc
    21901 tacgccgacg gcagcctcga ggcggcgcgc atcgcgcgca tgtccgagac gtgcggcgcg
    21961 gcgtgcctgc tggtgttccc gccgcagagc atgatcatgg gcggccagct ccgccccgag
    22021 atggcgctcg gccacttcaa gaccatcgcc gccgccaccg acctgccgat tatcgtgttc
    22081 cagtacccga tgacctcggg cctcggctat ccgttcgaca ccctgctcag gatggtcgac
    22141 gaggtgccga ccatccgcgc gatcaaggac tggtcgaacg atgccatgct gcatgagcgc
    22201 cacacccgca cgcttcagtc cttggcgcgc ccgatcaccg tgctcaccac ccacagcgcc
    22261 tggttgatga gctcgctggt gatgggcgcg caggggctgc tgtccggcgc cggcagcgtc
    22321 attcccgatc ttcaggtcgc gctgttcgaa gcggtgcagg ccaaggacct cgcccgcgcg
    22381 caggcgatca acgatcgcat ctatcctttg tcgcaggtgt tctacgcgcc gccgttcctc
    22441 gacatgcaca accgcatgaa ggagtgcctg cgcctgctcg gccgcctcga caaggcggtg
    22501 gtgcgcccgc cgctgatgaa gcttagcgag gccgagatcg cgcgcctgca acaggcgctc
    22561 gatgaggccg cgatcgcgcc cgagggtgcc ctcggcctcg ccgccgagta ggcccggccg
    22621 aggactacgc gccttcgccc cgcgcgcgtg cgtgcggggt cgcgcgtcat catgaccggg
    22681 ctgttcgacc ccgggcggat cggcccggtc gagataccga accgcatcgt catgccgtcg
    22741 atgaccacgc gcacggccga cgaggcgggc ttcgtcaccg acgacaccgt tgcctacttc
    22801 gccgcccgcg cgcgcggcgg ggtgggcctg gtcacggttg agatgtcggc gcccgagccc
    22861 gccggccgcc atcgcctgcg cgagctcggc atcgccgacg accgctttgt gcccggcctc
    22921 gggcgcctgg tcgcggcgat ccacgacgcc ggcgcgcgcg cggcgatcca gcttggccat
    22981 ggcggcggcc acacccgcgc ggacgtgtgc ggcgagccgc cgatcgcgcc ctccgctgtg
    23041 ccgcatcaag tcttcgagcg caccatggag acggtggttc cggtggcgat gacgcgggcg
    23101 cgcatcggcc agaccacccg ggcgttcgtc gatgcggcgc tgcgcgcgca agcagccggc
    23161 ttcgactgcg tcgagatcca cgccgcccac ggctacctga tctcgcagtt cctgtgcccg
    23221 gcggagaaca cgcgcaccga cgactacggc ggcgcgctgg agaaccgggc gcgcttcggc
    23281 ctcgacatcc tgcgccgggt caaggcggcg gccccggggc tcggggtcat cttccggctc
    23341 acggtcgacg actacttcgc cggcggcatg cccgcggccg acggcatccg cgtcgccgag
    23401 tgggccgcgg ccgccggcgc cgacgcgatc cacgtctcgg cgggccacta ccgctcgctg
    23461 ccggcgacca tgatgatccc gccgatggcc atgcccgagg cgaccttcct gcacctcgcc
    23521 gcggcggtga aggcgcgcgt cgcggtgccg gtgatcgcgg tcggccggct tggcgatccc
    23581 gcgctggcca aggccgcggt cgcggacggc aaggccgact tcgtcgcgct cggccgcggc
    23641 ctgcttgccg accccgactg gcccgacaag gtccggcgcg gcgtcgcgcc ccggcattgc
    23701 ctcgcctgcc tcgcctgcat cgacggcatg cgcgcgggca gccggctgca ctgcatcgtc
    23761 aacccgacca ccgggcgcga gcgcgccttc gccgaggcgc gcccgcccgc gggcgagcgc
    23821 atcgccgtga tcggcgcggg gccggcgggc ttgtcctatg ccgagctcgt cgccggcgcc
    23881 aaccgggtca cggtgttcga gcgcgccaag gtgctcggcg gcgcgttgag gctcgccgcc
    23941 cgggcgccgc tgttcgagaa cgtcgccgcc gcgccggcat cgttcgccgc ctacatatcc
    24001 gcgctcgagg ccgcgtgccg ggcaaagggc gtcgacatcc gcaccggcac cgatccgatg
    24061 cgcgatccgc gcctgctcga cgggttcgac cgggtggtca tcgccaccgg cgccggctat
    24121 cggttctggc tcaggccggt ggtgcgcgcg ctgctcgtga ccggcctcgc tcgcctgccg
    24181 gggctgcgcg ccctgctcgc ccgcccgcgg ctgcgcgact ggttctacac ccgtgcgcgt
    24241 gcggggcgcg gcgccgccct cgcagggcgc gcgcctcccg gccaggtggt cgaggtaatc
    24301 ggcgatgcgc gccgggccgg caagaccggc gccgccgtgc gcgacgcctt cgaggccgcc
    24361 cttctccgcc ccgcagtgcg cggctgaacg aagggaaacc tgtcaaaacg gagctggact
    24421 ggcggcggcg tcattcctgc gcaggtaacg tcccggaaac gggcgtgctt tcccgggcct
    24481 ggtcggcatc cgaagtgacg acgacctgct cgggtcgctc ccgcgcgtcg agcggaaaga
    24541 cgcgcgcggc gcgcgcgcga atgaaaacgg actgcttgcc ctcgagcccg agggcctcga
    24601 attcggcacg agacaacgtc gcctcgacga ccggccgctt ccctccgatc gagagttcca
    24661 cgctgaccag cggcccggtg cgaatcacct cgcgcacgat ggcaggaagg cccaggcgcg
    24721 ccttgtccag cacgatctcg acatcgtccg gccgcacgta ggcgatcgcc gggccatccg
    24781 gcgccccgtg cgcgtcggca atggcgaagt cggtgtcgca gaccaccgcc gtcccggcac
    24841 ggatcacgca gctgaatcga ttcacgtcgc caaggaactc gtagacgaac ggcgtcgccg
    24901 gatggtcgta gatctcagcc ggcgcgccca cctgcgcgat tcggccctgc tccatcacga
    24961 ccaccgtgtc ggcaagctcc agggcttcct cctgatcgtg ggtcacgaac acgctggtga
    25021 gccgcatcct gtcgtgaaga cgcctcagcc aacgtcgcag ctctttcctg accttggcgt
    25081 ccagtgcccc aaacggctca tcgagcagca ggacgcgcgg ctcgatcgcg agcgcgcgcg
    25141 cgagcgcgac gcgctgcctc tggccgcccg atagctgcga ggggtagcgc cgtgcgaatt
    25201 gatcaatctg cactaagtgc agcagctcca gcacccgcct gcggatagcg gcctcggaga
    25261 tgcgctcgcg gcgcgccttg acgcgcagcc cgaaggccac gttctcgaac accgtcatgt
    25321 ggcggaacag cgcgtaatgc tggaacacga agccgacctg gcgctcgcgc acgtggcggg
    25381 cgcttgcgtc ctcgccgtcg aacagcaccc ggccctggct cggaaagtcg agcccggcga
    25441 tgatccggag cagggtcgtc ttgcccgacc ccgacggccc gagtaaagcc acgagctcgc
    25501 cgggatcgat tcgcaggctc acgtcgtcga gggcggtgaa gcggtcgaac cgcttcgtga
    25561 cgtgctggac ctcgatggtc atgacggtgc tccctaacgc cgccgcgtgg ccgcgagctc
    25621 ggcgccgtag cgccactcca ggaccgcctt cagcgccagc gtcaccaacg caaggaaggc
    25681 gagcagcgag gccacggcaa aggcggcggc gaaatcatac tcgttgtaga ggatttccac
    25741 atggagcggc acggtgttgg tgagcccgcg gatgtggccc gagaccacgg agacagcccc
    25801 gaactcgccc atcgcgcggg cgttgcacag cagcacgccg tagagcagtc cccacttgac
    25861 gttggggagc gtgacgcgcc agaacgtctg ccaggcgccg gcgcccagcg acaacgccgc
    25921 ctcctcttcc tccgtcccct gttcctgcat gagcgggatc agctcgcgcg cgacgaacgg
    25981 aaaggtgacg aagaccgtcg ccagcacgat tccgggcacg gcgaagatga tctggacgcc
    26041 gtgctccgcg agccacgggc cgaaccagcc ctgggccccg aacagcagca cgtagaccag
    26101 gccggcgatc acgggcgaga ccgagaaggg aaggtcgatc agggtagtga gcacgctctt
    26161 gccaagaaac tcgaacttgg cgattgccca ggccgcggcg agcccgaagg cgacgttgag
    26221 cggcacggcg atggcggcgg cgagcagagt gagtcgaatt gctgcggcgg cctccggctc
    26281 ggtgacggcg gcgaggtaag cgtccacgcc ggcgcgcaat gcctcggcga agaccgtgac
    26341 cagcggcagc aacaggaaga gcgagaggaa ggccaaggcg agcgcggtca gcgcccagcg
    26401 cacacccgcc gattccgtgg ccacccgagc cctgccgagg ttttcaaact gggtggtcat
    26461 cgctccgtcc gatcactccg catcgcccgg cacgcggggc cgatacgacc gaccctagcc
    26521 gctaccattc acgcgcgccg catgtcaccg tggaaaattc acccgtgcca tcgcgggcgt
    26581 ttccccgaaa ccaccatttg cccattcgtc tcaggctggc gccatgcgca cccggctcca
    26641 gtgctgaaga aggttgatca ggagcaggag cacgaacgag gccagcagca tcacggtgcc
    26701 gatcgcggtc gcgccggcgt aatcgtattg ctccagtttg atcacgatca ggagcggggc
    26761 gatctccgat accatgggta ggttgccggc gatgaagatg accgatccgt actcgcccac
    26821 cgcccgggcg aaggcgagcg ccacgccggt gagcaccgct ggcgcgatcg agggcaggat
    26881 cacgcgcagg aagatttgca ggcgattcgc cccgaggctc gccgctgcct cctcgagctc
    26941 gacgtcgaag tcctggagca cgggctccac ggtgcggacc acgaacggca gcccgatgaa
    27001 cacgagcgcg accacgacgc cggacggcgc gaacgccacc ttcattccga gtggcgcgag
    27061 gaactgcccg atccatccgt tctcggcata gagcgcggcg agcgagatgc cggcgacggc
    27121 ggtcggcagg gcgaacggca gatcgaccac gccgtccacg atgcgctttc ccggaaagcg
    27181 gtagcgcacc agcacccagg cgacgaggac gccgaagacg gcgttgatca gcgcggccag
    27241 cgccgaggcg ccgaagctca gccgatacgc ggcaagcgcc cgcggcgcga agaccgcggc
    27301 gagaaagtcg gccgcgccca tgccggcggt gcgcatcacc atggcggcga gcggcacaag
    27361 cacgactagg ctcagataca ggagggtgaa gccgagcgcc ggcccgaagc cgggcaggat
    27421 gctcgggcgg cgaaccctag agggcatgcg tggcctcgcc gagtgccgcc gctaccggca
    27481 cgctcgcggg cgtcgcgccc cgcgcggctg cggcgggccg aggcagggtg ctgcgcatac
    27541 tcccgatctc accattcgtc gagggcgacc gcagccgcgc cgggacccgg cgatatgaca
    27601 gtggcggcgg tcgcgaatca acggcgccgt cgcggccacg cccgcgccgc cccggccgcg
    27661 ggcggcccta ttgcgtcgtc tggtagatgg cgtcgaagat gccgccgtcg gcgaagtgct
    27721 tccgctgcgc ggcctgccag ccgccgaacg cctcgtccac ggtgaaacgc tcgaggtcgg
    27781 ggaagcggtc gaggtcctcg ggccgggcgt gctgcggttc atgcgggcgg taatagtgag
    27841 ctgcagcgac gcgctggccg gccggcgcgt agaggtactc gacataggcc cgggcaaggt
    27901 cgagcgtgcc gtgcctctcg gcgttggcct cgaccaccgt caccggcggc tcggcgagga
    27961 tgctgaccga gggaacgacg atctcgaact tgtcggggcc aaattcctcg acggctagaa
    28021 atgcctcgtt ctcccaggcc acgagcacgt cgccgatgcc gcgctggacg aaggtgttgg
    28081 tcgagccccg cgcgcccgtg tccaatacgg ggacgttcct atagatggcg ccgacgaact
    28141 ctcgcgcctt gccctcgttg ccgctccact tgcggagcgc gtagccccaa gccgcgaggt
    28201 agttccaccg cgccccgccc gaggtcttcg gattcggtgt gatgacctgg acgccggact
    28261 tcacgaggtc gtcccagtcc ctcactccct tggggttccc cttgcgcacc agaaacacga
    28321 tcgtcgaggt gtagggcgcg ctgttgtgcg gcaaccgtaa ttgccagtcc ttcggaatca
    28381 ggccggagcg tgccgcgatc tcgtcgatgt cgtaggccag cgcgagcgtc accacgtcgg
    28441 cgggaagccc gtcgatgacc gagcgcgcct gctttccgga accgccgtgg gactgcctga
    28501 tcgtgaccgt ctccccggtc gtctctttcc aatggctcgc gaattccgcg ttgaactcct
    28561 gatagagctc gcgcgtcgga tcgtacgaga cgttgaggat cgttcgatcc gcccaggtcg
    28621 gagtgggcgc gagcagcact gccgccgcca tcgcggcaac agcgaaccgt cgtccgcaaa
    28681 ggcttgtccg aaacatgcac tgtgtccttc gctgctcagc cgctcacgcg ccgcggctgc
    28741 gcatgtagcc gagggtggac agcggcgcga tggcgacggc gttgttgcga taggtcgacc
    28801 agctgtcgta gatgcgccgg ttgatgtcgc cgtccgcggc ggtctcggcg accacctcgg
    28861 cgctgatctc gaaccagcgc ttgagcacgt cgtcgggaag cgggccgatg gtgacgtcgt
    28921 gctcggcgac cagcctctgc agcgcgaccg cgttctcgaa gtcgaactcg gacagcagca
    28981 gcgccgcctc gaccgtcgcc gaattgcgga cgatcgcctg caagtccttg ggcagcgccc
    29041 ggtacgcctc gaggttgacc atcagcgact gcgcggtgcc gggctcgtgc acgcccgggc
    29101 cgtagcagtg cttggcgacc ttgtaaaagc cgaaggcgag gtccatccac ggcccgcccc
    29161 attcggcggc gtcgatcacc cccgactgaa gcgccggcat gatctcgccg ccgggcaagt
    29221 tcaccgtcgt cgcgcccatc cggttgagca cctccgcctg aagcccggcc atgcggatct
    29281 tgagcccctt cacgtcgtcg agcgagcgca cttggttgcg gaaccagccg accatctgca
    29341 tgccggcatt gccggcgagc cacgcgcgca ggccgaagcc ctcatacagc tcgtcccaca
    29401 gcgcctggcc gccgccgtaa tagatccagc ccatgtgctc gtcgggcgtg agcccgccgg
    29461 gcaccgatga gaagaacgcc gagctgcggt tcttggcgat ccagtaatag ggcgaatcgt
    29521 ggccgagctc ggcgttgccg tcgcgcaccg cgtcgaacac ctcgaatgcg ggcacgaact
    29581 cgccggccgc gaacagcttc acctcgagcc ggccgtcgga catctcgccg atgcggtcgg
    29641 cgatgcgctg cgcggcgatg ccgacgcccg ggaagttgcg cggccagcag gtgaccatgc
    29701 gccagctcac ccgcccttgc gcgatcgccg gggccggcat cgccgccgcc gcgagtcctg
    29761 cgcccgcgcc cttgagcaat ccgcgtcgac tggttggttt ccccgacatg tcgtggcctc
    29821 cctgcttgcc gccggttgcg gccctccccc gccagcatgg cgcaagtaac cggacggcta
    29881 caagcccgcc gccccgcttg ccggccgggc cgcaaacgcg ccacagtggc gcccgcagcg
    29941 acacccagca cggagcgagc gatggccgag ttccccctca aggtcggcga ccaagtcacc
    30001 ttcgccaaga cggtcggcga gaccgacatc tacctgttcg caggcatcac cggcgacttc
    30061 gccggcaacc acgtcaacga ggagtacatg aagggctcga gctatggccg gcgcatcgcc
    30121 cacggcgcgc tcctgatcgg ctacatgtcg accgcctcga ccatgatgat caatacctgc
    30181 ctgcgggccg acatgaccga gaccccggtc tcgctcggct acgaccgggt gcgcttcacc
    30241 gccccggtgt tcatcaacga cacggtcacg gtcacctacc ggatcaagga caccgacccg
    30301 gacagccgcc gcagccggtc gcagataacg gtcaccaatc agaccggcga gaccgtcgcg
    30361 gtcgccgagc acatcctcaa atgggtgccg aaccagcggt cctagcgcgg gcgaggaacg
    30421 cctcgacctc ggcgcggcgg gggatcggcg cgaccgcgcc gtagccgatg gtagcgagcg
    30481 cggcggcggc gttggcgtag gcggccgcgg cgagcggatc gcgcccggcg acggtctcgg
    30541 cgagaaacgc gccgtcgaac gcgtcgcccg cgccggaggc atcgacgcag gcgaccgcat
    30601 ggccggcgat ccggcggtgc tccgctcccg ttgcgaccag tacgccttcg gcgcccatct
    30661 tcaccgccac cacgcgcgcg ccgaggtcga gatacgccgc ggcgatttca gccacccgct
    30721 cgcgcccggt cagcagccgg ccctcctcgg cgttggtcag cacgtaccag gcctgtgcgg
    30781 cggtggcgtc gatcaccgca cgcgcgcgct cgagcggcca cagcctgagc ctgaggttgg
    30841 gatcgtacga gacccgcacg ccgctctcgt tggcgagcgc gaccgcgtgg gccacggcct
    30901 cgtacgcgct ggcgctgatc gcctggctga tgccggagac gtgcagcacc ttggcgtcgg
    30961 cgatcatgtt tccgggcagg tcggcggcgc gcatccggct cgcggcgcta cccgtgcggt
    31021 aataggtgaa gtggtggccg ctggtgtcgt gggtgatgaa gtagacgccg gtctgggcct
    31081 ccgggtcgcg tttcaccccc gacgtgacga ttccctccgc gcgccacagc tccaggaacg
    31141 actcgccgaa cggatcggcg ccgagggcgg tgacgtagcc gacgcgcgcc ccctggcggg
    31201 cggcggcgat ggcgcagttc gaggtatcgc cgccgtaacc ggcgagccag cggttgccgc
    31261 cggaatcggg caggcgggtg aactcggcca gcggctcgcc cagacagacg atgtccgccg
    31321 gcacggccgc gctcaccccg cgggcaggcc gagcgaggcg cgcaggctct tgccgagtgc
    31381 cgtgatctcg ccgctccgcc cggccaggat caggcgctcg tcgaccagcc gcccgcccat
    31441 gccgatgaag gccgcgcccg cgtcgagata ctccagcacc tgctcggcgc ggatgccccc
    31501 tgtcggcacc agcggcacgc cggggaagat cgacctgagc gcactgacat gcgccggacc
    31561 gccggcgctc gagaccggaa agatcttgac cgcatcggcg ccggcggcgc gcgccgccag
    31621 cacctcggtc ggggtcagcg cgccgagcag caccggcacc ccggccgcat ggcacggcgc
    31681 gacgaccgcg gggacgacgc acggcgagac cacgaagcgc gcgcccgcgg agaggcaacg
    31741 ttcggcgtct tgctcgctgg tcacggtgcc ggccccgacc agcacgcccg gccgagccgc
    31801 gagcgactcg atcagcgccg gcgcgtcggc gatggtcatg gtgatctcga acacgtcgaa
    31861 gcccgcgtcg gcgagcgcat ctaccgcctg ctccgcagtg gtcgccgcgg acgtgcgtat
    31921 caccggcacg atcttggcct cgaccagccg gccgagaaga ccgccgatgt ccgccgatgg
    31981 cgccgcccga ttcacgcgcg cgcgcccttc gtccgcccgg agtcgattgg ccgcctatcc
    32041 attgccgcct cagaatgccc gcaccggcac ctcggcacct cgccgctcgg ccgacagata
    32101 ggcgctgtag atggtcgaga tgcagtcggc ggcaagccgc gaatcgctct cggcctcttc
    32161 gccatacgcg accgtgcggt agaacgcctc gatctcctgc gggaagccgg tcatctggtc
    32221 ctcgtccggg gcggtgaact gccagccctg cttggtgccg gtcttctcga ccacgtagat
    32281 gtccttgaag gtggcgtcca ccgggttgta ggtgagcatg gcggtgttgg ggttgatgtt
    32341 gcagatcgcg cggtggttgt tggcgcatac ctcgagccag ttgtggatgc cgcccatcac
    32401 gatctccgag gcaaagatgt cggccacggt gccgtcctcg aaggtcacgt ggatcgccgc
    32461 gaagtcctcg atgtcgtgat agtcggtgcg caggtggccg gcgtcgcggt agccctcgag
    32521 gcgggtcagc tcgtgggtgc gcgcgctcac tgtcgccggg cggatcggcc ggccgtcgcg
    32581 ggcgcggccc tcgacccgct tcaggtacag cgccgcgccc agcgggtggc aggccttgcc
    32641 gatcatcgag ccgccgccgc ccagccgcca gaagccgtag gccttggagt gcgaccccga
    32701 atgcgcttcc tcgccgtgca gccacaggat ctgggcgccg gtcttctcga cgatctcgcg
    32761 ctccttctgg atcgccggcg catggaccca gttctcggcg tagaggatgc gcccgcggct
    32821 cgcctgctcg gccgcgagca tgcgctcgat gctcgccagc gcaccggcga gcgccgcctg
    32881 cttgggcgcg ccgcggccgt cgaaatcggc cgagccgtcg ccgaagtagc cggtcagcgg
    32941 tttttccacg atcgcgaacc tgtcgcgcgc gagcgccgcc accgcgatcg gctcgtgggc
    33001 ggccggcggc acgcagacgt ggacaacctc agacttgtcc agcacctcct cgatgctgcc
    33061 gagcgcggcc agcccgtggg catcggcgaa ccggcgcgcg ttcgcgggcg tcggcgagta
    33121 caccccgacc aggtcgacgt ctgtgccgta gaccctccgg atgccgtgga cgtggagcgc
    33181 ggcggcgaag ccggacccga ccaggccggc gcggacggtc ttcctgctca ccagcttccc
    33241 tccccgcatg gcgccgccgg ggcttgacag tttgcacatc cgcggccgaa gcgctaacca
    33301 agattcgcgc ctcggcgcgc gatggagggc ggcggcgtat gcacgggacg gtcggtcttg
    33361 atcggcgcgg gtgggctcgc gccctcgccg ccgcggtggt cgccgcactc gcgctcgcgg
    33421 cctgctccga gagcgacgag gacaaggcga ctgcggccta tgagcgcggc gacttccaag
    33481 ccgcgggcgc cctcgccggc gcgcttgccg cggccgggaa cccgcgcggg ttggagctgc
    33541 tcgccctgat ggccgcccag ggcctgggac gcgcgatcga ctatggcgaa gcgttcgcgc
    33601 tgatcgaccg cgcgatcgcg ctcaacccgt cctatgcgga cacgcgcgcc gcgatcgagg
    33661 ctcgtcgcac cgccgcgacc gcggcctccg ccaaggcctt cgataccggc gactacgagc
    33721 gcgcctacga catcgccagg ccgctcgccg cattcggcga tgacgacagc gcccggctgg
    33781 tcgacgccct ggtcaccggc cactacatcc gcctgccggg atcggacatg tcctggcgac
    33841 ggttctggga gcaatgcagc ggcaataccc ggttcgagac cgaggccgag gccgaggctg
    33901 ggttcgatag cggctgcgcc gggctgcatg cggtctggga tggactggtg gtccgcgccc
    33961 tgcccggcga gcttcacgtc aagatgactc ccggccgccc ggcggcgcac cacgacatca
    34021 ctctgatgct cgacccgcct cccgacccgg agctcgcctc gcccgggcgc aaggtgcggt
    34081 tcgcgggcgt ggtcgccgca cgcggcacgc cgagccggcc cgacgtgctc gcccaagggg
    34141 cgatcatcgg cccggcgccg ctgaccgccg aagaggccag ccatgagcag acggtggttc
    34201 ttcaggcggt gatgaacgcc tgccagacgc tggtcgagga gcgcttccgc gccgagctga
    34261 tgccgcaatg ggcgatcgag gtcgaggcgc aggtgcgcgc cggcggcagc ccgcgcagcc
    34321 gcgccttctc gctgttcgtc ggcatcacct cggggcctga cgcattcgcc cgccaggccg
    34381 acgggacatg gcacggcagg ttcgacggca ctgtgaccat ccagagcgtc gttgcgcgca
    34441 ccgcccaggt gaccgagtac accgccgact gcaccatcga cggcgcgtac cggcgcggcg
    34501 acaggccggg cgagcacggc gtgctcagct tcgtccgggt tgccgagccg ctggtcgaat
    34561 cggcgcccgc gcgcctgcgg tagggacggg cgaagagatt tggctgtccg gctaacgaac
    34621 ggcctacgcc gcccgcgttc agttgcgctc gccctgctcc gggctcggcg tccaggccgc
    34681 ggcgcgcttc tcggcctcgg cgatctgttc cggggtcagg aagtccttga gcacgtcgag
    34741 gtggccctcg gcgccttcgc gcccgcgctc ggccgcgagc aggaaccact tgtacgactc
    34801 gatcaggtcc tgctcgatgc cgcgtccctc gtcgtacatg tagccgaggt tggtcatcgc
    34861 gtcgggcagg ccttgctcgg ccgcccgccg gtaccatatg accgcttcct tctcgttggg
    34921 aggcacgccc cggcccttgc ggtacatgaa gccgatgttg ttctgggccg tcgcgtcacc
    34981 cgcctcggcc aacggcagcc actccttgaa cgcggtttcg tagtcgcgca cggcgtaggc
    35041 gcggttgccg gcgtcgaagt cggccaggct cgcggacgca gccccgatca tgagcgcggc
    35101 aatgcacgcc aaggccgcct gcctcaaacg tcccggccgc gattgcttgc ggtccatgat
    35161 tgggccgact ccgccgatca gtgcccgccg ccgccgccgc cggcaacgtc gagcttgagc
    35221 tgtcggcaca tcttggattg gccgaagaac agctcgcagt ccgccgtctc gagatcgtcc
    35281 gaccggccct tgaaatgctc gacgatgaac tccgcgacgt gcttgacttg acggtggttc
    35341 agcaactggc gcgcgggtcc gggcgccatc tcgccgagtt cctcgagcgt catgccgtca
    35401 tagcagtcgt gatccttgta ggcgccccgc aagtgataca tcatccccgt tccgggccga
    35461 ccgcaggaga tcacttcgat catctggtcg agctcgagaa tggtctggcg gaaattgatc
    35521 ggcgtgccgc catagcccgg gccgcccatg ccgtgccatt tgtggcaggt gatgcagccg
    35581 gcctgcttgt agatcgcggc gcccgggagc ctgcgttgca tctgcgccga tgccggtgca
    35641 atccacgcca tcgccagcag accggcgagc gccaccgccg caccgcgcat tgacttgccg
    35701 caagtcctca ctcgttcctc cctggcgcgg tcgcaccgcc cctccggtcg cgctccgccc
    35761 ccccgacagc ggacgcatag gctcgcatgt actgcaaccg cccgttccag acaacccccg
    35821 ccgccgggcc gacctgagcg cgtcgcgggg cacaccgcgc agtcaattct gcttgaagtt
    35881 ccagcagcgc gaattttgcc gcccgccctc ggccagcctc tcaggctgtc ccgactcgaa
    35941 ggacctgacg agggccaggg catcgaaatg acagtggcgg ggaatgtatt ccttgagcag
    36001 aaggtcgtac ttctctttgg cgtccttgac gccgcccttg gcggcgagcg tcagccacgc
    36061 gtaagccgag atcacgtcgc gcggttcgcc gacgccgttg gcgagcatga tgcccagctc
    36121 atactgcgcc tgcgcatcgc cggccagcgc cgcggccagc cattcctgat gggcggtgtt
    36181 gaagtcgccg gcgtcgtagg cgtgcttgcc ggaaatgaag tcggcgcgca ccggcgctcc
    36241 gagccacagt accagcagcg ccagcgcgac gctcgcggcg cgccgccatg tccgcgtcat
    36301 ggtcttgccc tctcgccgcc cgaacgcgcg acggcccgcg tccgtcatcc aaacgcgggc
    36361 cgcccacgcc gaccgcggcc ggatcagctc tgttccatca gcgacatcgc cttcttgcga
    36421 tcttcgccga cgcaggtctt gagcgccgtc acgcatccca tcgagttgcc ggactggcac
    36481 tgctggagac ctgtcgtgca catctcgacc tgctcggcag aggtctgctc ctcagccgac
    36541 ggcccggcgg caacgcccga caccgcgaaa ccggcggtgg ccgcggccgc aatcaaagcg
    36601 atgaccgatt tcctcatgca atcctccctg acgctgcggc ccgaggctgt tggccacacc
    36661 gggcctgttc gcaccgtttc gttgtcgacg gcgcctttgc cgccgcctcc tccccgtaga
    36721 cgcaggcgcc gcacctgccc gccaaccgtt gcccgttgct ctgcggcaat cgatcatatt
    36781 cacccggccg gcgccacgca cggcctgcgt cgatcttgag gccggccaag agtatcaata
    36841 gtttccggca aacggaagag cgcaggcagc ctgccttaag gagcttgcgt cggggcgtcg
    36901 gcgccggcac cctgtcgggg cgcccatgtc agaccccagg gagacggcaa ggcgaacatg
    36961 acccccgatg cggcattcag gcgtgccggc gccggcggcc tgctcgcggt cgcggccgcg
    37021 gtgctggctt atggcggccc ggtcggcgcc ggccaggacg gcgacatccg cgagttcacc
    37081 gtcggcctga ccgtcgacga gctgcccgcg accggttatc tcggcttcaa gtgcggcaac
    37141 aacggcgcca agccgggccg ggagatcgcc ggctggaccg ccttcgccga gtgcccgccc
    37201 gacgacaagg gcctgcacga ggtggcgttc gagtacgacg attccgaagt ccggttcaag
    37261 gacttggagg gcaccgcgat cgccggccac gaggtcctga tctcgctcct gttcaacgac
    37321 caggggcagg tcgaggccat ccgcgtgttc accaacccgt tcgcgcggcc ctacgaaaag
    37381 cgccgggccc acatcctggc caacaaggtc aagcaccggt tcggcagcga gggctggacc
    37441 tgcgtcaacc agcagcttgc ggacggcgag tcggacatcg ccggcgtgtt catcaaggaa
    37501 agctgccgca aggacctcgg caaccggttc atcgaccttc acaccagctt ctttcgccgc
    37561 cacgggcccg agggcgagac catcgtcaac gccacgtctt tcgagatctg gcgcgaggcc
    37621 gcgccgtcct agccggccgc cggtgcgccg ccatccctga aaaaatgcgg cccctcggtc
    37681 gaggggccgc actcggtctc aaagggaccc cggccggtcg ccggccgggg cgctgtcgtc
    37741 caaagccgcc tagagcgcga acacccacag cacgttggcg ttgcggatct tctcaaggcc
    37801 cggagtcgac ggagcgaagt atgtcggcac cgtgccccag ccgcccgacg acacggcgat
    37861 gtactgcttg ccgtcgacgg caaagctcat cgtcggagcg tcggtcagcg tgttcatgtg
    37921 gaagcaccac agctcgtcca gcgattcatc gtcgaaggcg cagaacttgc cctcgtgctg
    37981 ggcgacgaac accagcccgc cggcggtgct gaggacgccc gccatcaccg gatagcgggt
    38041 gtcgaccttg cgcacgatcc tgccggtggt cacgtcgacc gcggtcaggc tgccggccag
    38101 gctcttgccg tcgctgctct tcgcggtggt cacgtccggc atgccgcccg agaaccactc
    38161 acggagcaca taggagccct cgggacccgg ggtctcctct tcgttgttga ccgtgatgca
    38221 gctctcgatg gtcgggatgt acgccatctt ggtgcgcggg ctgtaggaca tcggcatcca
    38281 gttcttgccg cccatcaggt tggggcagaa cgttgccgac ttgttggtcc ggttcggcgt
    38341 gtgctcggcc acgtagacct gcaggtcgga gttcggattg tagtcgagcg gtacgccggt
    38401 gaccttgtcc agaccgcgag tccagttgac ctccttgacg aacgggatgc cgtagatgaa
    38461 ctcgccgttg gtccggtcga tggcgtagaa gaagccgttg cggtcggcat ggataaacgc
    38521 cttccggtcg ttaccgccga tattgacgtt gacgaggatg ttctcgttga tgccgtcgta
    38581 atcgtacggg tcgttgggcg tgtactggaa gtggaaggcc accttgccgg tgtcggcgtc
    38641 gagcgcgaac atgcccgagg tgtacaggtt gtcgcccggg cgatactcgt tgtcccagtc
    38701 cgggcccgga ttgccgacgc cccagtagag caggttggtg tccgggtcgt aggagccggt
    38761 ctgccaggtc gagccgccgc cggtcagcca accatcgttg tcgtccttcc aggtctcgtg
    38821 ccccggctcg ccgggccccg gaatggtgta gcgacgccag acctcgttgc cggtgttgag
    38881 gtcgaccgcg gccagccagc cgcggatgcc gtactcggcg cccgacacgc cggtgatcgc
    38941 catgtccttg accaccagcg gcgcgatcgt gaccgtctcg gcgaccgccg gatcggcgag
    39001 ctgcgtctcc cagaccagct cacccgtgtc cttcttgatc cgcagcatgc gcccgtcgag
    39061 cgtgttcatg atcaccgagt cgccccacac ggcgacgccg cgattgttga tcgcgcagca
    39121 ggcgacgttg cctgcccagt ccttgtcgac gcccggatca taggtccact tgaccatgtc
    39181 ggggccctgg cccgccttgc gcaggtcgat ggccatcagc tcgctccagc cgttggccat
    39241 gtacatcgtg cctgactcga cgatcggcgt gccctcgatg ttgccgtagc gccagaagcc
    39301 gccgccttcg gtgccgccga gagcgatcgc ccaggccacc ttcaggtcct tgacgttcga
    39361 cttgttgatc tggtccagcg gcgagtaacg atcggaattg taggtccgat ggacggtcag
    39421 ccagtcgtcg gcgttcttgg ttgcgttcaa aaggtcgtca gccgtagtca tctcggcggc
    39481 cgacgccggc agcgacatgg caagcgcgag caagccggcg cccaccgctg caagggtcgc
    39541 agccggtttg gtcactttct ctttcatcag agcatcctcc ccatagacgt tcgcccgcgc
    39601 gcctagatca gccgggcttt cggccacccc actcggggct cgcccgaccc ggcgtcccgg
    39661 cggtacggtt ttgctggccg cgcaccgggc atcacgcgcg ccggagatgc gtaaaagtct
    39721 gacaatcgcc ggcggctgag tcaattgccg atcgcggcgg agtaaccgtc tggttaagaa
    39781 caatcggaga acggcatagg cgttgccatc aatctgcgtg cggcgcgggt tccgccgcgt
    39841 atcggcttgt gcgagacttg gccaaccagc ccgcgccgac ctcgccgcct gtcatggaaa
    39901 gccacccgat cgactgcgcg cctgccctgc cggagcggtt cgaggacatc gaagcgctcg
    39961 aggacgtcct ctcggcgccg accgcgcagc tggtcgagga cctcggccgg ctcgacggcg
    40021 atgtattggt gctcggggtc ggcggcaaga tggggccgac gcttgcgcgg ctcgccaggc
    40081 gcgcggcgcc ggccaagcgc gtggtcgggg tggcgcggtt caccgacaaa gccttgcgcg
    40141 cgcgcctcga atcgtggggc gtggagacca tcgcctgcga cctactggac cgcgccgcgc
    40201 tcgccgccct gccgcggctc gccaatgtcg tgttcatggc cgggcgcaag ttcggcgcca
    40261 ccgccgccgc cgccgacacc tgggcgatga acgctctggt cccggccgcc gtcgccgagg
    40321 ccttcgccgc atcgcggatc gtcgccttct cgaccggctg cgtctatccg tacctgccgg
    40381 ccgccggcgg cggcgccacg gagaagaccg cgcccagccc gccgcccggc gactacgcct
    40441 ggtcgtgcct ggcccgcgag cggctgttcg agtacgcctc gcgccgcttc gccaccgcgg
    40501 gccggctgat ccggctcaac tacgccatcg acatgcgtta cggcgtgctc cacgacatcg
    40561 cccgcgcggt gttcgccggc gcgcccgtcg atctcgccac cggccatgtc aacgtgatct
    40621 ggcagggcga cgccaacgcg caagcgttgc gtgcgctggc tcactgcacc tcgccgacct
    40681 cgccgctcaa cgtcaccggg cccgagacgg tcagcgtgcg cgccctggcc cacgccttcg
    40741 ccgagcggtt cggcaagccg ccgcgctttg ccggcaccga gtcggcgacc gcgtggctca
    40801 gcgacagcac cgccgcccgc cgcctgttcg gcgcgccgcg ggtgccgttg tcgcgcatga
    40861 tcgactggac cgcggactgg acgctgcgcg acggcgcgag ctacgacaag ccgacccggt
    40921 tcgaggtccg cgacggacgg ttttgatgga cgtccttgcg ccgcacacgc tgcccgcgcg
    40981 cctcgacgac gaggccgcgc tcgaggatct gctgtccacg ccgagccagg cgctgatcga
    41041 cgacctcgcg cgcctcgacg gcgacatcgc ggtgctcggc gtcgccggca aggtcggccc
    41101 gacgctggcg cggctcgcca agcgcgcggc gccggacaag cgggtgatcg gcgtcgcccg
    41161 gttctcccag cccgagatcc gcgaacgcct cgagtcatgg ggcgtcgaga ccatcgcctg
    41221 cgacctgctc gaccgcgacc aagtcgccgc gctgcccaag ccggccaatg tcgtgttcat
    41281 ggccgggcgc aagttcggca ccaccggcga cgagccgctg acctgggcga tgaacgtgct
    41341 cgttccggcg atcgtcgccg aggcgttcgc ggcctcgcgc atcgtcgcca tctcgacctt
    41401 gtgcgtctac ccgttcaccc cggtcgccga cggcggcgcc ggcgaggaca cggtgtgcgc
    41461 gccgcccggg gagtacgcca actcgtgcat cgggcgcgag cgcgccttcg agtacgccgc
    41521 cgccgtccac ggcacgcgag gccggctgat ccgcctggcc tacgccatcg acatgcgcta
    41581 cggcgtgctc cacgagatcg cgagccgggt gcgcgcgggc gagcccgtcg accttgccac
    41641 cggccacctg tcgctgatct ggcagggcga cgcc
//
LOCUS       DASWHU010000241         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1228388, whole genome shotgun sequence.
ACCESSION   DASWHU010000241 DASWHU010000000
VERSION     DASWHU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228388"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="rho"
                     /locus_tag="VGB88_08920"
     CDS             complement(<1..1116)
                     /gene="rho"
                     /locus_tag="VGB88_08920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9647607.1"
                     /translation="MNLKELKLKTPTELLDYAEECGVENASTMRKQDLLFSILKQLAE
                     SDIAIFGEGVLEILQDGFGFLRAPEANYLPGPDDIYVSPSQIRRFGLRTGDTIEGEIR
                     QPKEGERYFALLRINTINFEPPEKARHKINFDNLTPLYPDERLRLEVEDPTIKDLTAR
                     VIDLVAPIGKGQRALIVAQPRTGKTMVLQNIAHSITANHPECYLIVLLIDERPEEVTD
                     MQRSVKGEVVSSTFDEPATRHVQVAEMVIEKAKRLTEHQRDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKLADKRTFPAIDIGKSGTRKEELLV"
     gene            complement(1358..1807)
                     /gene="hemJ"
                     /locus_tag="VGB88_08925"
     CDS             complement(1358..1807)
                     /gene="hemJ"
                     /locus_tag="VGB88_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEX9647608.1"
                     /translation="MAELLAAAYPWLKALHVIAVIAWMAGMLYLPRLFVYHCAAAPGS
                     AQSETFKVMERRLLRAIVNPAMVAAWGFGLALLAVPGAVDLASGWLHAKLALVLAMSA
                     LHGLMARWRRDFAADANRRPARFYRIANEVPTVLMIAIVILVVVKPF"
     gene            complement(1807..>2097)
                     /locus_tag="VGB88_08930"
     CDS             complement(1807..>2097)
                     /locus_tag="VGB88_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9647609.1"
                     /translation="ARAGRERRPLVVAPVAFVSEHSETLVELDITYRRLAEAAGVPAY
                     VRVPALGTQPAFIAALAALAGGAEAGAGPRSHLGRRLCPPGFAGCPCRQGGR"
BASE COUNT          322 a          704 c          757 g          314 t
ORIGIN      
        1 caccaggagc tcctccttgc gggtgccgga cttgccgatg tcgatcgccg ggaaggtgcg
       61 cttgtcggcg agcttgcggt cgagcacgat ctcggagttg ccggtgccct tgaactcctc
      121 gaagatcacc tcgtccatgc gcgagccggt gtcgatcagg gcggtggcga tgatggtcag
      181 cgagccgccc tgctcgatgt tgcgcgcggc gccgaagaag cgcttggggc gctgcagcgc
      241 gttggcgtcg acgccgccgg tcagcacctt gcccgacgac ggcaccacgg tgttgtaggc
      301 gcgcgcgagc cgggtgatcg aatcgagcag gatgaccacg tcgcgctggt gctcggtcag
      361 gcgcttggcc ttctcgatca ccatctcggc gacctggacg tggcgcgtgg cgggctcgtc
      421 gaaggtcgag ctgaccacct cgcccttgac cgagcgctgc atgtcggtga cctcctcggg
      481 ccgctcgtcg atcagcagca cgatcaggta gcactcgggg tggttggcgg tgatcgagtg
      541 ggcgatgttc tgcagcacca tggtcttgcc ggtgcgcggc tgggcgacga tcagcgcccg
      601 ctgccctttg ccgatcgggg cgaccaggtc gatcacccgc gccgtcaggt ccttgatcgt
      661 cgggtcctcg acctcgaggc gcagccgctc gtccggatac agcggagtca ggttgtcgaa
      721 attgatcttg tggcgcgcct tctccggcgg ctcgaagttg atggtgttga tgcgcagcag
      781 cgcgaagtag cgctcgccct ccttgggctg gcggatctcg ccctcgatgg tgtcgccggt
      841 gcgcaggccg aagcggcgga tctggctcgg cgagacgtag atgtcgtcgg gaccgggcag
      901 gtagttggct tccggcgcgc gcaggaagcc gaagccgtcc tggaggatct cgaggacgcc
      961 ctcgccgaag atcgcgatgt cgctctcggc cagctgcttg aggatcgaga acagcaggtc
     1021 ctgcttgcgc atcgtgctgg cgttctcgac cccgcactcc tcggcatagt cgagcagctc
     1081 ggtgggagtc ttgagcttga gttccttgag gttcatggaa accgtcgtgt tggggaaaag
     1141 ggaagggatg gaaggttgcc cgcaacgccg cgggccttgc caccggcccc ggggcgggct
     1201 gcgaccagcc ggggcggcgg atcgcgaaga tggggccggt atgcggaagg acaggaaggc
     1261 gcgaccccgg ccggggccgc cgacgccact ctagcgatta gatggtgcgc gaggcaagca
     1321 tatccaagcc cccccgcgcg aaagcctgcc gccgcgctca gaacggcttg accacgacca
     1381 ggatcacgat cgcgatcatc agcacggtcg gcacctcgtt ggcgatacgg tagaagcgcg
     1441 ccgggcgccg gttggcgtcg gcggcgaagt cgcgccgcca gcgcgccatc aggccgtgca
     1501 gcgcgctcat cgccagcacc agggcgagct tcgcgtgcag ccagccggag gcgagatcga
     1561 ccgctcccgg caccgccagc agcgccaggc cgaaccccca tgcggcgacc atcgccgggt
     1621 tgacgatcgc gcgcagaagc cgcctctcca tgaccttgaa ggtctcggac tgcgccgagc
     1681 ccggcgccgc ggcgcagtgg tagacgaaca gccgcggcag atacagcatg cccgccatcc
     1741 aggcgatcac cgcgatcacg tgcagcgcct tgagccacgg ataggccgcg gcgagcagct
     1801 ccgccatcag cgccctccct gccggcacgg gcagcctgcg aagccgggtg ggcacagccg
     1861 gcggccgaga tgcgagcgcg gcccggcgcc cgcctcggcg ccgccggcca gcgcggcgag
     1921 cgcggcgatg aacgccggct gggtgccgag cgccggcacc cgcacataag cgggcacgcc
     1981 ggcggcctca gcgaggcggc ggtaggtgat gtcgagctcg accagggtct ccgagtgctc
     2041 agacacgaac gccaccggcg ccaccaccag cggccgccgc tcgcgcccgg cgcgcgc
//
LOCUS       DASWHU010000242         4211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3097965, whole genome shotgun sequence.
ACCESSION   DASWHU010000242 DASWHU010000000
VERSION     DASWHU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097965"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGB88_08935"
     CDS             <1..349
                     /locus_tag="VGB88_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX9647610.1"
                     /translation="AYAQRDPLNEYKREAFELFDDMLARFREMVVMTLAHVEIRLAEP
                     PPPELTRTPEPALAAAGGNGSAPAPRRPERALAGAAAGRDPRQPQSWGKIGRNEPCPC
                     GSGRKYKHCHGRV"
     gene            complement(549..1505)
                     /locus_tag="VGB88_08940"
     CDS             complement(549..1505)
                     /locus_tag="VGB88_08940"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX9647611.1"
                     /translation="MNTFLDFEKPIAELEGKIKELSHLSDSGELNIVDEVARLQSKAE
                     RLLKQTYAKLTPWQKTLVARHPDRPRFTDYVEQLVEDFTPLAGDRAFGDDAAVIGGLG
                     RWRGYAAVVIGHEKGHDTESRVKHNFGMARPEGYRKAVRLIRLAERFALPVITLVDTP
                     GAYPGIGAEERGQAEAIARSIETCLELRTPLISVIIGEGGSGGAIALAAANHVAMLEN
                     AIYSVISPEGCASILWRTAEAAQDAAEALRLTAQDLLKLGVIDAIVPEPVGGAHRGPE
                     SVLAAVGDEVERALRSLSALGGGELRQRRRDKFMAMGRQGLG"
     gene            complement(1544..2524)
                     /locus_tag="VGB88_08945"
     CDS             complement(1544..2524)
                     /locus_tag="VGB88_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9647612.1"
                     /translation="MSFTVVAQAPARLNRSELAVPGSRTELFEKAARSAADIVFLDLE
                     DAVAPADKAQARRNVIAAINEVDWGAKTLSVRINGLDTAYMYRDLVDVLEQAGERLDL
                     IMIPKAGTAADIYALDMLATQIEAAKGRAKRIGFEMIVESALGMANVEAIAAASPRNE
                     SLHLGVADYAASTRARTTSVGGANAAYGVLTDADPAGARAFHWGDMWHYALARLVVAA
                     RANGLRPVDGPFGDFRDADGYVAQANRAAALGCDGKWAIHPSQIALANQTFSPSAAEL
                     AKARRILAAMEQALAEGKGAVALDGRLIDIASIKQAENLVRTAAQIAASA"
     gene            complement(2521..3450)
                     /gene="xerD"
                     /locus_tag="VGB88_08950"
     CDS             complement(2521..3450)
                     /gene="xerD"
                     /locus_tag="VGB88_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9647613.1"
                     /translation="MSRSPGGAAAVETFVEMLVAERGAATNTVAAYRRDLADVAAFLK
                     RRGRDLTDAAADDLRAYLGALGARGLSAPTVARRLSCLRQLYGFLVADGVRADDPSAT
                     IDSPRRARPLPKVLGEDEVDRLLAAARGAHGAQGLRLQALVETLYAAGLRVSELVGLP
                     LAALGGDRNVLIVRGKGSKERMVPLGEPAAEAIAAYLTVRPAFLGASRESRWLFPSRG
                     AGGHLTRHRLAQALKRLALDAGIDPDRVSPHVLRHAFASHLLAHGADLRTVQAMLGHA
                     DISTTQIYTHVLDARLKALVATHHPLANDGVPG"
     gene            complement(3447..>4211)
                     /locus_tag="VGB88_08955"
     CDS             complement(3447..>4211)
                     /locus_tag="VGB88_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647614.1"
                     /translation="AGSQADMPGPLGRALLFQASRAQQPPDRRAALLDALWAAGGEGP
                     GFGVLARAAGVSLLTISPDRGVAWFAPAAVRALVAAGRAETAQAWYLALAEVGALEPQ
                     ALAAQKGALPVLVAAGIGMGRQWEPAMAGQWWRGLPEGPDPGVRAETANRVLMLLDAL
                     GVRMGPEVWDLFAEAPARVVEEVPNLGIRYGMRDAAQAGRRGETVLYALISLGDGGAI
                     GAGALSLGSVVRSLRAVGLEDDARALALEALIEVGR"
BASE COUNT          555 a         1554 c         1537 g          565 t
ORIGIN      
        1 cgcctacgcc cagcgcgacc cgctgaacga gtacaagcgc gaggcgttcg agctgttcga
       61 cgacatgctc gcccggttcc gcgagatggt ggtgatgacc ttggcccatg tcgagatccg
      121 cctcgccgag ccgccgccgc ccgagctcac gcgcacgccg gagcccgcgc tcgccgcagc
      181 cggcggcaat ggcagcgcac cggcgccgcg gcgtcccgag cgcgcgctcg ccggcgccgc
      241 cgccgggcgc gatccgcgcc agccgcaaag ctggggcaag atcgggcgca acgagccgtg
      301 cccgtgcggc tccggcagga agtacaagca ctgccacggc cgggtgtgat accggtgagc
      361 tcttgcgatg actcgccgtc tttcgccgtc tttgcgagcc cccgacttga tcgggggcga
      421 agcaatccag cactcggaac ccgcaagcct ggatcgcttc gtcggctccg cctcctcgcg
      481 atgacgacgg cggcgcgtgt ttatttaagc ttgaattgaa atgccgtgcc cggcgcgcgg
      541 gctggacgtc agcccaggcc ctggcggccc atggccatga acttgtcgcg ccggcgctgg
      601 cgcagctcgc cgccgccgag ggcgcttaag ctgcgcaggg cgcgctcgac ctcgtcgccg
      661 accgcggcca gcaccgactc ggggccgcgg tgggcgccgc cgaccggctc gggcacgatc
      721 gcgtcgatca cgcccaactt gagcaggtcc tgggcggtca gcctgagcgc ctcggccgcg
      781 tcctgggcgg cctcggcggt gcgccacagg atcgaggcgc agccctcggg cgagatcacc
      841 gagtagatcg cgttctcgag catggcgaca tggttggcgg cggcgagcgc gatggcgccg
      901 cccgagccgc cctcgccgat gatgacgctg atcagcggcg tgcgcagctc gaggcaggtc
      961 tcgatcgagc gcgcgatcgc ctcggcctgg ccgcgctcct cggcgccgat gccgggatag
     1021 gcgcccggcg tgtcgaccaa ggtgatcacc ggcagcgcga agcgctcggc gagccggatc
     1081 aggcggaccg ccttgcggta gccctcgggc cgcgccatgc cgaagttgtg cttgacccgg
     1141 ctttcggtgt cgtggccctt ctcgtggccg atgaccaccg ccgcgtagcc gcgccagcgc
     1201 ccgagcccgc cgatcaccgc ggcgtcgtcg ccgaacgcgc ggtcgccggc gagcggcgtg
     1261 aagtcctcga ccagttgctc gacatagtcg gtgaaacggg gccggtcggg gtggcgcgcg
     1321 accagggtct tctgccacgg ggtgagcttg gcgtaggtct gcttgagcag ccgctcggcc
     1381 ttcgactgca ggcgcgcgac ctcgtcgacg atgttgagct cgccgctgtc gctgaggtgg
     1441 ctcagctcct tgatcttgcc ttcgagctcg gcgatcggct tctcgaagtc gaggaaggtg
     1501 ttcatgccgc tagtctacca ttcgcgtcaa tccgggtcag ccgtcaggcg gaggcggcga
     1561 tctgcgcggc ggtgcgcacc aggttctcgg cctgcttgat cgaggcgatg tcgatcagcc
     1621 ggccgtcgag cgcgaccgcg cccttgccct cagcgagcgc ctgctccatc gccgcgagaa
     1681 tccgccgcgc cttggccagc tcggccgcgg acgggctgaa ggtctggttg gcgagcgcga
     1741 tctggctcgg gtggatcgcc cacttgccgt cgcagccgag cgccgcggcc cgattcgcct
     1801 gggcgacata cccgtccgca tcgcggaagt cgccgaacgg gccgtcgacc gggcgcaggc
     1861 cgttggcgcg cgccgccacc accaggcggg cgagcgcgta gtgccacatg tcgccccagt
     1921 ggaaggcgcg cgcgcccgcc gggtcggcgt cggtgagcac gccgtaggcc gcattggcgc
     1981 cgcccaccga ggtggtgcgc gcccgggtcg aggcggcgta gtcggcgacc ccgagatgga
     2041 gcgattcgtt gcgcgggctc gccgccgcga tcgcctcgac gttggccatg ccgagcgccg
     2101 attcgacgat catttcgaaa ccgattcgct tcgcccgtcc cttggcggcc tcgatctggg
     2161 tggccagcat gtcgagggcg tagatgtcgg cggcggtgcc ggccttgggg atcataatca
     2221 ggtcgaggcg ctcgcccgcc tgctcgagca catcgaccag gtcgcgatac atgtacgcgg
     2281 tgtcgaggcc gttgatccgc accgacagcg tcttcgcgcc ccagtcgacc tcgttgatcg
     2341 ccgcgatcac gttgcgccgc gcctgcgcct tgtcggcggg cgcgaccgcg tcctcgaggt
     2401 cgaggaacac gatgtcggcc gccgaccgcg ccgccttctc gaacagctcg gtgcggctgc
     2461 cggggaccgc gagctcgctg cggttgagcc gcgccggcgc ctgggcgacg accgtgaagc
     2521 tcatcccggc actccgtcat tggccaacgg gtggtgggtc gcgaccagcg ccttgagccg
     2581 cgcatccagc acgtgggtgt agatctgggt ggtcgagatg tcggcatggc cgagcatcgc
     2641 ctggacggtg cgcaggtccg cgccgtgggc gagcaggtgg ctggcgaagg cgtggcggag
     2701 cacgtgcggc gacacccgat cggggtcgat gccggcgtcg agtgcgagcc gcttgagcgc
     2761 ttgggcaagg cggtggcggg tgaggtgccc gccggcgccg cgcgagggaa acagccagcg
     2821 cgattcgcgg ctggcgccga ggaacgccgg ccgcacggtc agataggcgg cgattgcctc
     2881 ggccgcgggc tcgccgagcg gcaccatccg ctccttcgac cccttgccgc gcacgatcag
     2941 cacgttgcgg tcgccgccga gcgcggcgag cggcaggccg accagctcgg acacccggag
     3001 cccggcggca tacagcgtct cgaccagcgc ctggagcctg agcccctgcg ccccgtgcgc
     3061 accgcgcgcg gcggcgagca ggcggtcgac ctcgtcctcg ccgagcacct tcggcagcgg
     3121 gcgggcacgg cgcggcgaat cgatcgtcgc gctcgggtcg tcggcgcgca cgccgtcggc
     3181 aaccaggaag ccgtagagct ggcgcaggca ggacagccga cgcgccaccg tcggcgccga
     3241 cagcccgcgc gcgccgagcg cgcccagata ggcgcgcaag tcgtccgccg ccgcgtcggt
     3301 caggtcgcgc ccgcgccttt tcaggaaggc ggcgacgtcg gcgaggtcgc ggcgataggc
     3361 ggcgaccgtg ttggttgccg cgccgcgctc ggcgaccagc atctcgacga acgtctcgac
     3421 tgccgccgcc ccgcccggcg atcggctcat cggccgacct cgatcagggc ctcgagcgcg
     3481 agcgcgcgcg catcgtcctc gagcccgacc gcgcgcaggc tgcgcaccac cgagcccaag
     3541 ctcagcgcgc cggcaccgat ggccccgccg tcgccgagcg aaatcagcgc atagagcacg
     3601 gtctcgccgc ggcgcccggc ctgggccgcg tcgcgcatgc cgtagcggat gccgaggttg
     3661 ggcacctcct cgaccacgcg cgccggcgcc tcggcgaaca ggtcccagac ctcgggcccc
     3721 atgcgcacgc cgagcgcatc caacagcatc aaaacccggt tggcggtctc ggcgcgcacg
     3781 ccggggtcgg gcccctcggg caggccccgc caccactggc cggccatcgc cggctcccac
     3841 tgccggccca tgccgatgcc ggcggcgacg agcaccggca gcgccccctt ctgggcggcg
     3901 agcgcctggg gctcgagcgc gccgacctcg gccagcgcca ggtaccaggc ctgcgcggtc
     3961 tcggcgcggc cggccgcgac cagggcgcgc accgccgcgg gcgcgaacca ggcgacgccg
     4021 cggtccgggg agatggtcag caggctgacc ccggcggcgc gcgccagcac cccgaacccg
     4081 ggcccctcgc cgcccgccgc ccacagcgca tcgagcagcg ccgcgcgccg gtcggggggc
     4141 tgctgggcgc ggctcgcctg gaacaacagc gcccggccga gcggccccgg catgtccgcc
     4201 tgcgagcccg c
//
LOCUS       DASWHU010000243         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1229381, whole genome shotgun sequence.
ACCESSION   DASWHU010000243 DASWHU010000000
VERSION     DASWHU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229381"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGB88_08960"
     CDS             complement(<1..243)
                     /locus_tag="VGB88_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647615.1"
                     /translation="MRWLLRVLRGGLVLAAAAAAGCGAAAADSVLKVVPHADLKNIDP
                     IWTTAYITRNHGYMVYDTLFALDEKLEVRPQMVERWE"
     gene            390..1721
                     /locus_tag="VGB88_08965"
     CDS             390..1721
                     /locus_tag="VGB88_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9647616.1"
                     /translation="MAPVNVPPSALRGSACPTFVASGWNGAADRLSQAARAATSRPRP
                     MPVLLLIAFVDLVGFGIVIPLLPFYAERFGASPAEVTLLMAAYSLAQFATAPVLGALS
                     DRYGRRRVIVVSLAGAAASYVLLGLAGSLAALFAARALAGAMAGNIAAVQAAIADLTA
                     PEDRARGMGRFGAAFGLGFIVGPFVGGVLAGVGAGADGSPNFALPPLAAAAASTLALV
                     LAVLVLPETRQARAPAPAAGTRAGVLALWLGPLAGAVERADLRVLVAVLFLVVFAFAG
                     LESTFALWAERALAWGPREVGILFAGAGAIAAVIQGGAIGPLARRLGEERLMLLGIAT
                     LACGFAALAVSATVPAAALAMALLAAGFALANPTLHSLVSRRAAPGRTGASLGVAQAA
                     SSLARILGPAAAGVLFTAAGRAAPYVAGAAILVVVGAGLWRRARRRAADLA"
     gene            complement(1612..2742)
                     /locus_tag="VGB88_08970"
     CDS             complement(1612..2742)
                     /locus_tag="VGB88_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647617.1"
                     /translation="MRTRNRNLRTPYAPVAALAGLALALAACAPRSQEVGPRLDAPRI
                     EADAIIAADGARLPLRSWLPEGDARAVVLGVHGFNDYGHGFAMPAAVWAEAGIAVYAY
                     DQRGFGAGPAPGLWPGVDALVGDLAEAARVVGARHPGTPLYLVGESMGGGVVMSALAR
                     GLVPEAEGAVLVAPAVWGRQTMNAFYRAALWLAAHTVPWATVTGEGLDITPSDNQEML
                     RALSADPLVIKNTRIDSLWGVVDLMDSALAAAPAIETPLLVLYGARDEVIPEAPTRRM
                     LCMLDGRRTVAVYPDGWHMLLRDLQAEVVDRDVIAWIEQRPRRLPSGADSNLARFADC
                     GTAPDALRRGRRRAGGHAATGPRRPPPGSPHRRRRARRGRRR"
     gene            2784..3626
                     /locus_tag="VGB88_08975"
     CDS             2784..3626
                     /locus_tag="VGB88_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEX9647618.1"
                     /translation="MSFLDRISECNRHDPAGYVPFTVDGVRLGQVRRDRLTDLAGFPG
                     LIADQAGGVRLAEGVRGRAARSALMERVGRALAARGLCERWRDEPYPVGPRFGEVWFE
                     IERALAEVFGVCAYGVHLNGYVAGDGAPAIWIPRRALDRPTYPGELDNTVAGGQPANL
                     SVADNLVKEAWEEASIGPGLIARARPVGTLSYRLDGRFGLKRELIFAYDLELPPDFTP
                     ENRDGEVEDFRLWPADRVMQAVAESRDFKFNCALVLIDFFVRHGLIGPDHPDYVAICA
                     GLRQ"
BASE COUNT          402 a         1366 c         1345 g          518 t
ORIGIN      
        1 ctcccagcgc tcgaccatct gcgggcggac ctccagcttt tcgtcgagcg cgaacagggt
       61 gtcgtagacc atgtagccgt ggttgcgggt gatgtaggcg gtggtccaga tcgggtcgat
      121 gttcttgagg tcggcgtggg ggaccacctt gagcacgctg tcggccgccg ccgcgccaca
      181 gcccgccgcg gccgccgccg ccagcaccag cccgccgcgc aggacacgca atagccacct
      241 catgaccgat ccctccctgc cgccggtgcc tcaggtcgcg cctcgaatag acacgatccg
      301 ggacgcgaat tccaggcggc tccgacgccg gattcacgcg gaggcgcgga gacgcgggaa
      361 tctcgccgat catctcggca gctattttct tggcacccgt caacgtcccg ccctcagcgc
      421 tccgcggctc cgcgtgccca acctttgtcg cctccggttg gaatggcgcc gccgatcggc
      481 tatcacaggc cgcccgcgcc gccacctcac gcccgcgccc gatgcccgtc ctgctgctga
      541 tcgccttcgt cgacctggtc ggtttcggca tcgtgattcc gctgctgccg ttctatgccg
      601 agcggttcgg cgcctcgccg gccgaggtga cgttgctgat ggcggcctac tcgctggccc
      661 agttcgcgac cgcgccggtg ctgggcgcgt tgagcgaccg ctacggccgg cggcgggtga
      721 tcgtggtgag cctcgccggc gccgcagcca gctacgtgct gcttggcctg gccggctcgc
      781 tcgccgcgct gttcgccgcg cgcgcgctcg ccggcgccat ggccggcaac atcgccgcgg
      841 tccaggccgc gatcgccgac ctcacggcgc cagaggaccg cgcccgcggc atgggccggt
      901 tcggcgccgc gtttgggctc ggcttcatcg tcggcccgtt cgtgggcggc gtgctggccg
      961 gggtcggcgc cggcgccgac gggtcgccca acttcgcctt gccgccgctg gcggcggcgg
     1021 cggcgagcac gctggcgctg gtgctggcgg tcctggtcct gcccgagacg cgccaggcgc
     1081 gcgcgccggc gcccgcggct ggcacgcgtg ccggcgtgct cgcactgtgg ctcgggccgc
     1141 tggcgggggc tgtcgagcgc gccgatttgc gcgtgctggt ggcggtgctg ttcctggtgg
     1201 tgttcgcctt cgccgggctc gagtcgacct tcgcgctgtg ggccgagcgg gcgctcgcct
     1261 ggggcccgcg cgaggtcggc atcctgttcg ccggcgccgg cgccatcgcc gccgtgatcc
     1321 agggcggggc gatcgggccg ctcgcccggc gcctcggcga ggagcggctg atgctgctcg
     1381 gcattgccac gctcgcctgc ggcttcgccg cgctggcggt gagcgccacc gtgccggcgg
     1441 cggcgctggc gatggcgctg ctcgccgcgg gcttcgcgct cgccaacccg accctgcaca
     1501 gcctggtctc gcgccgcgcc gcgcccggac gcaccggcgc cagcctcggc gtcgcccagg
     1561 ccgcaagcag cctggcgcgc atcctcggcc cggccgcggc cggggtgctg ttcaccgccg
     1621 ccggccgcgc cgcgccctac gtcgccggtg cggcgatcct ggtggtggtc ggcgcgggcc
     1681 tgtggcggcg tgcccgccgg cgcgccgccg acctcgcctg agcgcatccg gcgctgtgcc
     1741 acagtcggcg aagcgggcga ggttcgagtc cgcgcccgag ggcaggcggc gcgggcgctg
     1801 ctcgatccag gcgatcacgt cgcggtcgac gacctcggcc tggaggtcgc gcagcagcat
     1861 gtgccagccg tcgggataga ccgcgaccgt gcgccggccg tcgagcatgc acagcatgcg
     1921 ccgggtcggg gcctcgggga tgacctcgtc gcgggcgccg tagagcacca acagcggcgt
     1981 ctcgatggcg ggagcggccg ccagcgcgct gtccatcagg tcgaccacgc cccacagcga
     2041 atcgatccgg gtgttcttga tcaccagcgg gtccgcgctc agcgcgcgca gcatctcctg
     2101 gttgtcggag ggcgtgatgt cgaggccctc gccggtcacc gtcgcccacg gcacggtgtg
     2161 cgcggcgagc cacagcgcgg cccggtagaa agcgttcatg gtctgtcgcc cccacaccgc
     2221 gggcgccacc agcaccgcgc cctcggcctc gggcaccagg ccacgggcga gcgcgctcat
     2281 caccacgccg ccgcccatgc tctcgccgac caggtagagg ggcgtgcccg gatggcgggc
     2341 gccgaccacg cgcgcggcct cggcgaggtc gccgaccagc gcgtctaccc ccggccacag
     2401 cccgggcgcg gggccggcgc cgaagccgcg ctggtcgtag gcgtagaccg cgatccccgc
     2461 ctccgcccac accgccgccg gcatggcgaa gccgtggccg tagtcgttga agccgtggac
     2521 gccgagcacc accgcgcgcg cgtcgccctc gggcagccag gagcggagcg gcaggcgggc
     2581 gccgtcggcg gcgatgatcg cgtcggcctc gatgcgcggc gcgtccagcc gcggcccgac
     2641 ctcctggctg cgcggcgcgc acgccgcgag cgccagcgcg aggcccgcga gcgcggcgac
     2701 cggggcatag ggcgtgcgga ggtttcggtt tcgggttcgc atcggtccgg tcatgggcgt
     2761 aggctgggca attccgaccg ccgatgagct ttctcgaccg catcagtgaa tgcaaccgcc
     2821 acgacccggc gggctatgtg ccgttcacgg tcgatggcgt gcggctcggc caggtgcgcc
     2881 gcgaccggct cacggacctc gcgggcttcc ccgggctgat cgccgaccag gcgggcggcg
     2941 tgcgcctggc cgagggcgtg cgcgggcgcg cggcgcgcag cgcgctgatg gagcgcgtcg
     3001 ggcgggcgct cgccgcgcgc gggctttgcg agcgctggcg cgacgagccc tatccggtcg
     3061 ggccgcgctt cggcgaggtg tggttcgaga tcgagcgcgc gctcgccgag gtgttcgggg
     3121 tgtgcgccta cggcgtccac ctcaacggct atgtcgcggg cgacggcgcg cccgcgatct
     3181 ggatcccgcg gcgcgcgctc gaccggccga cctatccggg cgagctcgac aacacggtcg
     3241 cgggcggcca gccggccaac ctgtcggtgg ccgacaacct ggtcaaggag gcatgggagg
     3301 aggcctcgat cggccccggg ctgatcgccc gtgcgcgccc cgtgggcacg ttgagctacc
     3361 gcctcgacgg ccgcttcggc ctcaagcgcg agctgatctt cgcctacgac ctggagctcc
     3421 cgcccgactt cacgccggag aaccgcgacg gcgaggtcga ggacttccgg ctgtggcccg
     3481 cggaccgcgt catgcaggcc gtcgcggaga gccgcgactt caagttcaac tgcgcgctcg
     3541 tcctgatcga cttcttcgtc cgccacggcc tgatcggccc cgaccacccc gactacgtcg
     3601 ccatctgcgc cggcctcagg caatgagcga g
//
LOCUS       DASWHU010000244         9704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3099140, whole genome shotgun sequence.
ACCESSION   DASWHU010000244 DASWHU010000000
VERSION     DASWHU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3099140"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VGB88_08980"
     CDS             <1..652
                     /locus_tag="VGB88_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9647619.1"
                     /translation="ETMASAPAGEDGFMHPYRLIGAVNAALDAEAVVAVDGGDILSFA
                     RIALRDCRYLDPGPLGCLGVGVPFAVAAALACPGRKVVALTGDGAFGFNALELDTAAR
                     HGARAVFIVANNGAWNIEAYDQKVNWGGRLSGSLLRRSDYAGLARALGLHAERIEDPD
                     ALPAALARAFADAPALLDVQVTRDAVSPDGRSGLAAVPDLQPLATWDAAERARRGS"
     gene            776..1192
                     /locus_tag="VGB88_08985"
     CDS             776..1192
                     /locus_tag="VGB88_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX9647620.1"
                     /translation="MTTAGDAIRVLIAKVGLDGHDRGVKVVARTLRDAGMDVIYTGLH
                     RSPEEVVAAAVQEDVDVLGISLLSGAHMTIFPRVVELLRAAQADDIILVGGGVMPDED
                     VDALKRLGVAEVLLQDTPPEAVVAAIRRLVAERGPR"
     gene            1207..2649
                     /locus_tag="VGB88_08990"
     CDS             1207..2649
                     /locus_tag="VGB88_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9647621.1"
                     /translation="MEDWSFPPAYDEAYLPTRDSRYWFPARETMDAGAREAAIIVRLR
                     QVMAYARDRSPFYRRKWDEAGIHPDHITSLEAFERVPVVTKAELRASQAAHPPFGDYL
                     CVGMSEVHHIHGTSGTTGRPTAFGISRADWRAIANAHARVLWGMGVRPGDRVFIAAIF
                     SLYLGSWGAMLGAERLGCQTFPFGAGAGGMTARAASWLAAMKAEAFYGTPSYALHLAE
                     VAREEGLDARDFGLKVMFFSGEPGASIPGIRRRIEDAYGARVFDCGTMAEMTPFMSAA
                     GTAEGGPGMVLWQDMVYHELCDPATFRRVAYGAQGTPVYTHLERTSQPMIRLLSGDVS
                     RWQAPPAPCGRSYPTFPDGVFGRIDDMFQVRGENIYPSEIDTALNGLDGYGGEHRIVI
                     SRAGAMDELLVRVEVAPEVAAASAQPAFAGRCAAALQKLLGVRAAVEAVAPGTFPRTD
                     FKARRVIDDRDLFRELNARLERQDASHGAG"
     gene            2636..3616
                     /gene="meaB"
                     /locus_tag="VGB88_08995"
     CDS             2636..3616
                     /gene="meaB"
                     /locus_tag="VGB88_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9647622.1"
                     /translation="MARAEAKAVPEGDLVARVLAGETRALARMITYAETGDEAARPLL
                     AEVYRHGGRAHLVGITGVPGSGKSTLVRALAQALRADKRTVGVVAVDPSSPFSGGAIL
                     GDRIRMAELAGDDGIFIRSMASRGVLGGLAHATLDAVDVLDAGGFDVVMIETVGVGQD
                     EVDIVRVADTTVVVSAPGLGDDIQAIKAGVLEIADIHAVSKCDRADANRTVTDLKHML
                     TLGLKPGAGWRVPVLRTSAHSGDGVAELLAKIDGHRAYLVEHGELEARRRRSAEARVV
                     KIAEEIVRRRYARHGDGGATRSLVERVSARELDPHGAALQLLDALNRGGP"
     gene            3625..5316
                     /locus_tag="VGB88_09000"
     CDS             3625..5316
                     /locus_tag="VGB88_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9647623.1"
                     /translation="MSTIFDGAALEALEQAEREWEAGEVKDFVDRQPESRPAFRTASG
                     LPLKRAYTALDVADIPLDDIGLPGRYPFTRGPYPTMYRGRTWTMRQIAGFGTGADTNA
                     RFRYLLAQGQTGLSVDFDMPTLMGYDSDHPMAHGEVGREGVAVDTIDDLEALFDGIDL
                     RRISVSMTINPSAWILYAMYIALARRRGYDLDELSGTIQADILKEYIAQKEWIFPIRP
                     SVRLVRDCIVFGARNMRRYNPINISGYHISEAGATAVQEAAFTMANAIAYVEEVVKSG
                     LDVDEFAPRLAFYFVAQSDLFEEVAKFRAVRRVYAKIMRERFGARKPESMRLRCHAQT
                     AAATLTKPQHQVNIVRTAYQALAAVLGGCQSLHTNGMDEAFTIPTEEAMRIALRTQQV
                     IAEESNVAAVVDPLGGSYLVESLTSAIEREVFRILEQIDRMGGTIKAIEDGWFQREIA
                     DSAYDFALAKANGERTVIGVNKFVDADETPDIDTHAYDADTERRQIASLTRARAERDN
                     AEVARLMDELRAVARDTAENIMPATIALVDARASMGEIVDALKELWGSYRETPMI"
     gene            complement(5333..5701)
                     /locus_tag="VGB88_09005"
     CDS             complement(5333..5701)
                     /locus_tag="VGB88_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9647624.1"
                     /translation="MPNTLTSDDALRLWRTAIVDLVRADRPDLSMRQMAILVTVYTAP
                     PPHTVRGLARRLNIAKPAVTRALDRLCRLELVQRRRDDRDRRNVLVQRTVAGTMYLSD
                     LADMLTAAASGAQPGALTAA"
     gene            complement(5748..7145)
                     /locus_tag="VGB88_09010"
     CDS             complement(5748..7145)
                     /locus_tag="VGB88_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX9647625.1"
                     /translation="MVEAIVEEQVAGAVPITAVTEAAFAGWRGAAPAPVRNWLDACAV
                     KAKAGTQALVPGADGGLERVVAVVEDRPDLWSFAGLPAALPPGSYRLDAPAGFDLDAR
                     LATAAALGWLIGGYRFERYRKPADGAETPRLAAPKGADLARARRLARAHALVRDLVNT
                     PANDMGPAELAAAAREVAGARGAAITVIEGEALLDAGYPMIHAVGRASARAPRLIDLA
                     WGDAGAPRLTLVGKGVCFDSGGLDLKTADGMRLMKKDMGGAAHALALADAIMDANLAV
                     RLRVLIPAVENSVSGSAFRPGDVLATRAGRNVEIGNTDAEGRLVLCDALAEADREAPR
                     LLIDLATLTGAARVALGPDVPALFTDDDALAADLARHATGESDPLWRLPLWRGYRKDL
                     DSEVADICNISDAPFAGAITAALFLREFLEKTESWAHFDIMAWNRASRPGRPKGGEAV
                     ALRALYALVAERFAG"
     gene            7245..7508
                     /locus_tag="VGB88_09015"
     CDS             7245..7508
                     /locus_tag="VGB88_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647626.1"
                     /translation="MRIATLVCVLALAVAAPDPALAYIGPGAGITMLGALWGVLAAIV
                     LALAAVLAWPIRAAVRSRRRKARAEAPVPDAGAAPPAEAGTDR"
     gene            7572..7979
                     /locus_tag="VGB88_09020"
     CDS             7572..7979
                     /locus_tag="VGB88_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647627.1"
                     /translation="MPASTLATALVAALAGATSGAIGVVVYWRLSNQERIRDMKREAR
                     ARRSRLGADDILPGEAVRLALANLRQALVMLGRVAPPALAGGAVMVPAILWLDARAAA
                     LPAAETWYGGWELPFIAGALAAALAVKLAFKVE"
     gene            7993..9345
                     /locus_tag="VGB88_09025"
     CDS             7993..9345
                     /locus_tag="VGB88_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX9647628.1"
                     /translation="MSRAIFLGGLCALSFLVGAAAVTFEAFPYRQFREARFALDALVE
                     AMGGGLPAVFHGYDLAYDKPTAFGSRRAEAGPVLIAGGPYELTSRCPGQGCLAWITDR
                     RGEVAYSWPLDLEAAWGEFETVAAVGPLDRMYPVGLHLFDNGDLLVSFQSLGTFPWAV
                     GMAKVDATGRLLWKRENLGHHWFSIDEAGRIYVPAMRVVPSPIPLAAGGIEIVCELDY
                     IYEDLIQVLDRDGNLVKEFPLLNVLMDSGWPGLLHYSEDLSYQSCDPTHLNDVQVLPA
                     ALAAEYPGLAAGDLLISMRTNNALAVLDGDSGRVKWLLAGRTVAQHGPRFIGGNKIIV
                     FANKNGAGADNYSRLVEIDLGDGAMRTLFPTEATADSIRFHALVAGHLDVNPDRRHVL
                     VSLTGQGRVLELDLATGQVVWEYVNTHRVPGETRPIRMSALAAYYVESPAFLAPAHAR
                     "
     gene            complement(9403..9612)
                     /locus_tag="VGB88_09030"
     CDS             complement(9403..9612)
                     /locus_tag="VGB88_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9647629.1"
                     /translation="MPIGTVKWFNSTKGFGFIKPDEGTKDAFVHISAVQRAGLQGLSE
                     GQRIEFELVADRGGRLAADNLKLIG"
BASE COUNT         1314 a         3540 c         3488 g         1362 t
ORIGIN      
        1 cgagaccatg gcctccgcgc ccgccggcga ggacggcttt atgcatccct accggctgat
       61 cggcgcggtc aacgccgcgc tcgatgcgga ggcggtggtg gcggtcgacg gcggcgacat
      121 cctgagcttc gcgcgcatcg cgctcaggga ctgccgctac ctggaccccg ggccgctcgg
      181 ctgcctcggg gtgggcgtgc cgttcgcggt cgccgcggca ctcgcctgcc ccgggcgcaa
      241 ggtggtggcg ctcaccggcg acggcgcgtt cggcttcaac gcgctcgagc tcgacaccgc
      301 cgcgcgccac ggcgcgcgcg cggtgttcat cgtcgccaac aacggcgcct ggaacatcga
      361 ggcctacgac cagaaggtga actggggcgg gcggctcagc ggctcgctgc tccggcgctc
      421 ggactatgcc ggtctcgcgc gcgcgctcgg cctccacgcc gagcgcatcg aggacccgga
      481 cgcgcttccg gctgcgctcg cgcgcgcgtt cgccgacgcg cctgcgctcc tcgatgtcca
      541 ggtgacccgc gacgcggtct cgcccgacgg ccgctccggc ctcgccgcgg tgcccgacct
      601 gcagccgctc gccacctggg acgccgccga gcgcgcccgc cgcggctcgt agccccggtt
      661 ctggactggc ggcgccggtt ggcttatgga ctgatggaat acggaatacg taagaaagcg
      721 ccatgcgcgc cctcgccgac ccgcgcggtg cggcctgagc cgggagcgat cgaccatgac
      781 caccgccggc gatgccatcc gcgtgctgat cgccaaggtc gggctcgacg gccacgaccg
      841 cggggtcaag gtcgtcgcgc ggaccttgcg cgacgccggc atggacgtga tctacaccgg
      901 cctgcaccgc tcgcccgagg aggtggtcgc cgcggcggtg caggaggacg tcgacgtgct
      961 cggcatcagc ctgctctcgg gcgcgcacat gaccatcttc ccgcgcgtgg tcgagctttt
     1021 gcgcgcggcg caggccgacg acatcatcct ggtcggcggc ggggtgatgc cggacgagga
     1081 cgtggacgcg ctcaagcggc tcggggtcgc cgaggtgctg ctccaggaca ccccgcccga
     1141 ggcggtcgtc gccgccatcc gccgcctggt cgccgagcgc ggcccgcgct gagcgccgcc
     1201 gcggccatgg aagactggtc gttcccgccg gcctacgacg aggcgtacct gccgacgcgc
     1261 gacagccggt actggttccc cgcgcgcgag accatggatg ccggcgcgcg cgaggccgcc
     1321 atcatcgtcc gcctgcgcca ggtgatggcc tatgcgcgcg accgcagccc gttctaccgc
     1381 cgcaagtggg acgaggccgg catccacccc gaccacatca cctcgctcga ggccttcgag
     1441 cgcgtgccgg tggtgaccaa ggcggagctc cgcgcgtcgc aggcggcgca cccccccttc
     1501 ggcgactacc tgtgcgtagg gatgagcgag gtccaccaca tccacggcac ctcgggcacc
     1561 accggccggc cgaccgcgtt cggcatcagc cgcgccgact ggcgcgccat cgccaatgcg
     1621 catgcgcggg tgctgtgggg catgggcgtg cgcccgggcg accgggtgtt catcgccgcc
     1681 atcttcagcc tctatctcgg ctcgtggggg gcgatgctcg gcgccgagcg gctcggctgc
     1741 cagaccttcc cgttcggcgc cggcgccggc ggcatgaccg cgcgcgccgc ctcgtggctc
     1801 gcggcgatga aggcggaggc gttctacggc acgccctcat atgcgttgca cctggccgag
     1861 gtcgcgcgcg aggaaggcct cgatgcgcgc gacttcgggc tcaaggtgat gttcttctcg
     1921 ggcgagcccg gggcctcgat cccgggcatc cgcaggcgca tcgaggacgc ctacggcgct
     1981 cgggtgttcg actgcggcac catggccgag atgaccccgt tcatgtccgc cgccggcacc
     2041 gccgagggcg gcccgggcat ggtgctgtgg caggacatgg tctatcacga gctgtgcgac
     2101 cccgcgacct tccgccgcgt cgcctacggc gcgcagggga cgccggtcta cacccatctc
     2161 gagcgcacct cgcagccgat gatccggctc ctgtcgggcg atgtcagccg ctggcaggcg
     2221 ccgccggcgc cgtgcgggcg cagctatccc accttccccg acggcgtgtt cgggcgcatc
     2281 gacgacatgt tccaggtgcg cggcgagaac atctatccgt ccgagatcga cacggcgctg
     2341 aacgggctcg acggctacgg cggcgagcac cgcatcgtga tcagccgcgc cggcgccatg
     2401 gacgagctcc tggtccgggt cgaggtcgcg cccgaggtgg cggcggcaag cgcccagccg
     2461 gccttcgccg gacgctgcgc ggcggcgctt cagaagctcc tcggcgtgcg cgcggcggtc
     2521 gaggcggtcg cccccggcac cttcccgcgc accgacttca aggcgcggcg cgtgatcgac
     2581 gaccgcgacc tgttccgcga gctgaacgcg cgcctcgagc ggcaggatgc gagccatggc
     2641 gcgggctgag gcgaaggccg tgcctgaggg cgacctggtg gcgcgggtgc tggccgggga
     2701 gacgcgcgcg ctcgcgcgca tgatcaccta cgccgagacc ggcgacgagg ccgcgcgacc
     2761 gctgctcgcc gaggtctacc gccacggcgg gcgggcgcat ctcgtcggca tcaccggcgt
     2821 cccggggtcg ggcaagtcga cgctggtgcg cgcgctggcg caagccttgc gggcggacaa
     2881 gcgcaccgtc ggcgtggtcg cggtcgaccc gtcgagcccg ttctcgggcg gcgccatcct
     2941 cggcgaccgc atccgcatgg ccgagctcgc cggcgacgac ggcatcttca tccgcagcat
     3001 ggcctcgcgc ggcgtgctcg gcgggctggc gcatgccacg ctcgatgcgg tcgacgtgct
     3061 cgatgccggc ggcttcgacg tggtcatgat cgagaccgtc ggcgtcggcc aggacgaggt
     3121 cgacatcgta cgcgtcgccg acaccaccgt ggtggtctcg gcgcccggcc tcggcgacga
     3181 catccaggcg atcaaggccg gcgtgctcga gatcgccgac atccacgcgg tcagcaagtg
     3241 cgaccgcgcc gatgccaacc ggaccgtgac cgacctcaag cacatgctga ccctcgggct
     3301 gaagccgggc gccggctggc gggtgccggt gctcaggacc agcgcccaca gcggcgacgg
     3361 cgtcgccgag ctgctggcga agatcgacgg ccaccgcgct tacctggtgg agcacggcga
     3421 gctcgaggcg cgccggcgcc gcagcgccga ggcgcgggtg gtgaagattg ccgaggaaat
     3481 cgttcgccgg cgatatgccc gccacggcga cggcggcgcc acccgctcgc tggtcgagcg
     3541 cgtgtcggcg cgcgagctcg atccgcacgg cgcggcgctg cagctgctcg acgccctcaa
     3601 ccggggagga ccatgatgac caggatgagc accatcttcg acggcgccgc gctcgaggcg
     3661 ctcgagcagg ccgagcgcga gtgggaggcg ggcgaggtca aggacttcgt cgaccgccag
     3721 cccgaatcga ggcccgcgtt ccgcaccgcc tcgggactgc cgctgaagcg cgcctacacc
     3781 gcgctcgacg tcgccgacat cccgttggac gatatcggcc tgcccggccg ctacccgttt
     3841 acccgcgggc cgtatccgac catgtatcgc ggccgcacct ggaccatgcg ccagatcgcc
     3901 ggcttcggca ccggagccga caccaatgcg cgcttccgct acctgctcgc ccagggccag
     3961 accgggctgt cggtcgactt cgacatgccg accctgatgg gctacgactc ggaccatccg
     4021 atggcgcacg gcgaggtcgg ccgcgagggc gtcgcggtcg acaccatcga cgacctcgag
     4081 gcgctgttcg acggcatcga tctgcgccgc atctcggtgt cgatgaccat caacccgagc
     4141 gcctggatcc tgtacgcgat gtacatcgcg ctcgcgcgcc ggcgcggcta cgacctcgac
     4201 gagctgtcgg gcaccatcca ggccgacatc ctgaaagagt acatcgctca gaaggaatgg
     4261 atcttcccga tccgcccgag cgtgcgcctg gtgcgcgact gcatcgtgtt cggcgcgcgc
     4321 aacatgcgcc gctacaaccc gatcaacatt tcgggctacc acatctccga ggccggggcg
     4381 accgcggtcc aggaggccgc gttcaccatg gccaacgcca tcgcctatgt cgaggaggtg
     4441 gtgaagtccg gcctcgatgt cgacgagttc gcgcccaggc ttgcgttcta cttcgtcgcc
     4501 cagtccgacc tgttcgagga ggtggcgaag ttccgcgccg tgcgccgcgt ctacgccaag
     4561 atcatgcgcg agcgcttcgg cgcgcgcaag cccgagtcga tgcggctgcg ctgccacgcg
     4621 cagaccgcgg cggcgacgct gaccaagccg cagcaccagg tcaatatcgt gcgcaccgcc
     4681 tatcaggcgc tcgccgccgt gctgggcggg tgccagagcc tgcacaccaa cggcatggac
     4741 gaggcgttca ccattcccac cgaggaggcg atgcgcatcg cgctcaggac ccagcaggtg
     4801 atcgccgagg aaagcaacgt cgccgcggtg gtcgacccgc tcggcggctc gtacctggtc
     4861 gagagcctga ccagcgcgat cgagcgcgaa gtgttccgca tcctcgagca gatcgaccgc
     4921 atgggcggca ccatcaaggc gatcgaggac ggctggttcc agcgcgagat cgccgattcg
     4981 gcctacgact tcgcgctcgc caaggcgaac ggcgagcgca ccgtgatcgg ggtcaacaag
     5041 ttcgtcgacg cggacgagac gcccgacatc gacacccacg cttacgacgc cgacaccgag
     5101 cgccgccaga tcgcgtcgct cacgcgcgcg cgtgccgagc gcgacaacgc cgaggtcgcg
     5161 cggctgatgg acgagctgcg cgcggtcgcc cgcgacacgg ccgagaacat catgccggcg
     5221 accatcgcgc tggtcgacgc gcgcgccagc atgggcgaga tcgtcgacgc cttgaaggag
     5281 ctgtggggct cgtaccgcga gacgccgatg atctgagccg ggctcgcggg gcctaggcgg
     5341 cggtgagcgc tccgggctgc gcgccggacg cggcggcggt caacatgtcg gcgagatccg
     5401 acaggtacat ggtgccggcg acggtgcgct ggaccagcac gttgcgccgg tcccggtcgt
     5461 cgcgccggcg ctggaccagc tcgagccggc acagccggtc gagcgcccgg gtcaccgccg
     5521 gcttggcgat gttgagccgg cgggcgaggc cgcgcaccgt gtgcggcggc ggcgccgtat
     5581 agacggtgac cagaatcgcc atctggcgca tcgaaaggtc cgggcggtcg gcgcggacca
     5641 gatcgacgat tgccgtccgc cacaggcgca gggcgtcgtc ggatgtgagc gtattgggca
     5701 tgggccgtgc gctgtcctgg gctcgctacc gttgcagatt cgtaacgtca gcccgcgaaa
     5761 cgctcggcaa ccagcgcgta cagggcacga agggccactg cctctccccc cttcggtctg
     5821 ccaggtcgcg atgcgcggtt ccaggccatg atgtcaaaat gggcccacga ctcggttttt
     5881 tccaagaact cgcgcaaaaa aagcgcggcg gtgatggcgc cggcgaacgg cgcgtcggag
     5941 atgttgcaga tgtcggcgac ctcgctgtcg aggtccttgc ggtagccgcg ccacagcggc
     6001 agccgccaca gcgggtcgga ttcgccggtc gcgtggcggg cgaggtcggc ggcgagcgcg
     6061 tcgtcgtcgg tgaacagcgc cggcacgtcg ggcccgagcg cgacgcgcgc cgcaccggtg
     6121 agagtcgcca ggtcgatcag cagccgcggc gcctcgcggt cggcctcggc cagcgcgtcg
     6181 cacagcacca gccggccctc ggcgtcggtg ttgccgatct cgacgttgcg cccggcgcgg
     6241 gtcgccagca cgtcgcccgg ccggaaggcg ctgcccgaga cgctgttctc gaccgccggg
     6301 atcagcacgc gcaggcgcac ggcgaggttc gcatccatga tcgcatcggc gagcgcgagc
     6361 gcgtgcgcgg ccccgcccat gtccttcttc atcaggcgca tgccgtcggc ggtcttgagg
     6421 tcgagcccgc ccgaatcgaa gcacacgccc ttgccgacca gggtcagcct gggcgcgccg
     6481 gcatcgcccc aggcgaggtc gatcagccgc ggcgcgcgcg cgctcgcccg gccgaccgcg
     6541 tggatcatcg ggtagccggc gtcgagcagc gcctcgccct cgatcaccgt gatcgcggcg
     6601 ccccgcgcgc cggcgacctc gcgcgccgcc gccgcgagct cggccgggcc catgtcgttg
     6661 gccggcgtgt tcaccaggtc gcgcaccaga gcgtgcgccc gggcaagccg gcgggcgcgg
     6721 gcgagatcgg cgcctttggg cgcggccagg cgcggagtct cggcgccgtc agcgggcttc
     6781 ctgtagcgct cgaaccggta gccgccgatc agccagccga gcgccgccgc ggtcgccagg
     6841 cgggcgtcga ggtcgaaccc cgccggcgcg tccaggcggt agctccccgg gggcagggcc
     6901 gcgggcaggc cggcgaagct ccacaggtcg ggccggtcct cgaccaccgc gaccacccgt
     6961 tccagtcccc cgtcggcgcc cgggaccagc gcctgcgtcc ccgccttggc cttgaccgcg
     7021 cacgcgtcca gccagttccg aaccggcgcc ggcgccgccc cgcgccagcc ggcgaacgcg
     7081 gcctcggtca ccgccgtgat cgggaccgcg cccgcgactt gctcctcgac gatcgcctcg
     7141 accatgccgc ttctccgtgc ctcggttgga tttcgggtcg cggcgacagt acattagtca
     7201 cggtcgccat gaagcggcgc gaccgccacg gcaaggggtg tgacatgcgc attgcgaccc
     7261 tggtttgcgt gctggcgctg gccgtcgccg cgcccgatcc ggcgctggcc tatatcgggc
     7321 cgggtgccgg catcaccatg ctcggcgccc tgtggggcgt gctggcggcg atcgtgctgg
     7381 cgctggccgc ggtcctggcc tggccgatcc gggcggcggt gcggtcgcgg cggcgcaagg
     7441 cgcgggccga ggcgccggtg cccgatgccg gggcggcgcc gcctgccgag gccggcaccg
     7501 accgttaagg tggccgcgcc ggccgggtgc cgccgtcctg cccggcccgg gcgggcgcgc
     7561 gcgggctgag cgtgccggcg agcaccctcg caaccgcgct ggtcgccgcg ctcgcgggcg
     7621 caaccagcgg cgcgatcggg gtggtcgtct actggcggtt gtcgaaccag gagcgcatca
     7681 gggacatgaa gcgcgaggcc cgggcgcggc gcagccggct cggcgcggac gatatcttgc
     7741 ccggcgaggc ggtgcggctc gcgctcgcca acctgcgcca ggcgctggtc atgctcggcc
     7801 gggtcgcgcc gccggcgctc gccggcgggg cggtgatggt gccggcgatc ctgtggctgg
     7861 acgcgcgcgc cgccgccctg cccgcggccg agacctggta cggcggctgg gagctgccgt
     7921 tcatcgccgg cgcgctcgcc gcggcgctcg cggtcaagct cgcgttcaag gtggaatagc
     7981 cggcgccggc cgatgtcgcg ggcgatcttc ctcggcgggc tgtgcgcgct gtcgttcctg
     8041 gtcggcgccg ccgcggtgac cttcgaggcg ttcccctatc gccagttccg cgaggcgcgg
     8101 ttcgcgctcg acgcgctggt cgaggcgatg ggcggcggcc tgccggcggt gttccacggc
     8161 tacgacctcg cctatgacaa gccgaccgcg ttcgggtccc ggcgtgccga ggccggaccg
     8221 gtgctgatcg cgggcggccc ttacgagctt accagccgct gccccgggca aggctgcctc
     8281 gcctggatca ccgaccggcg cggcgaggtc gcctatagct ggccgctcga tctcgaggcc
     8341 gcgtggggcg agttcgagac ggtcgccgcg gtcggcccgc tcgaccgcat gtatccggtc
     8401 ggcctgcacc tgttcgacaa cggcgacctt ctggtctcgt tccagagcct gggcacgttc
     8461 ccgtgggcgg tcgggatggc caaggtcgac gccacggggc ggctgttgtg gaagcgcgag
     8521 aacctcggcc accactggtt ctcgatcgac gaggccgggc gcatctacgt gccggcgatg
     8581 cgtgtcgtgc cgagcccgat cccgctcgcc gcgggcggca tcgagatcgt gtgcgagctc
     8641 gactacatct acgaggacct gatccaggtg ctcgaccgcg acggcaacct ggtcaaggag
     8701 ttcccgctgc tgaacgtgct gatggactcg ggctggcccg gcctgctcca ttactcggag
     8761 gacctcagct accagagctg cgacccgacc cacctcaacg acgtgcaggt gctgccggcg
     8821 gcgctcgcgg ccgagtaccc ggggctcgcg gccggcgacc tgctgatctc gatgcgcacc
     8881 aacaacgcgc tggcggtgct cgacggcgac tcgggccggg tcaaatggct gcttgccggg
     8941 cggacggtgg cgcagcacgg cccgcgcttc atcggcggca acaagatcat cgtgttcgcc
     9001 aacaagaacg gcgccggcgc cgacaactac agccgcctgg tcgagatcga cctcggcgac
     9061 ggcgcgatgc gcaccctgtt cccgaccgag gcgaccgcgg actcgatccg gttccacgcc
     9121 ctggtcgccg gccacctcga cgtcaacccc gaccgccgcc acgtgctggt ctcgctcacc
     9181 ggccagggcc gcgtgctcga gctcgacctc gccaccggcc aggtggtgtg ggagtacgtc
     9241 aacacccacc gggtgccggg cgagacgcgg ccgatccgga tgagcgcgct cgcggcctat
     9301 tacgttgagt cgccggcgtt cctggcacct gcgcacgccc gctgagcggc cgttgcgcgc
     9361 gccggcggga catgcgccag gcgcccgcgg cggcgcgcgg cgtcacccga tcagcttgag
     9421 gttgtcggcc gccaatcggc cgccgcgatc ggcgaccagc tcgaattcta tcctctggcc
     9481 ctcgctcagg ccctgcaggc ccgcgcgctg gaccgccgag atgtgaacga acgcgtcctt
     9541 cgttccctcg tcgggcttaa tgaagccgaa ccccttggtg gaattgaacc acttgaccgt
     9601 cccgatcggc atgccttgct ccccgcctgc ggtcccggcc cgcggcggcc gtccgcagga
     9661 ttgccaaatc acatccggca tgcccgacgt gaccattgcc gccg
//
LOCUS       DASWHU010000245         5978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_504311, whole genome shotgun sequence.
ACCESSION   DASWHU010000245 DASWHU010000000
VERSION     DASWHU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504311"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VGB88_09035"
     CDS             complement(<1..656)
                     /locus_tag="VGB88_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9647630.1"
                     /translation="MGWLTVHRLATAIAVAMAVFHLGVALVGPPEALIFRGLHLGFAF
                     VLIFLLFPARQGTAWRLLLDGAPLAAGLAGIGHILVNYQYMVTRFPYVDELTDWDLWL
                     GVALVALVLEASRRVIGWALPVTAALFLGYAMLGPWLPFGLAHEGFPLADLVDQLYMT
                     TEGVFGIPLGVSATYVVLFVMFGAFLERVGTGELFMSFALALTGHTPGGPAKVACLTS
                     G"
     gene            complement(680..1660)
                     /locus_tag="VGB88_09040"
     CDS             complement(680..1660)
                     /locus_tag="VGB88_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9647631.1"
                     /translation="MTAHNAPAAGRPARARLTALAVSLALAAGAAGAQAADYRFMTGP
                     QGGSWYPLGGAISNLARDTLADSRLRVMPGGGITNVMAVEAGKAQFALSNVTAAVDAI
                     AGRPPFDGAAANIRHLATLYPQVFQFVVAADSGIQTVADMRGRAIAVGPRGHTGEQAS
                     RHVLDVYGLGYDDLSSVNHVGYTDAVSLIKDGHIDAFTVITTIPAGAVMDTAAGRPIR
                     VLSIPADKLEALKAHNPQYVPRTIPANTYPEQTEEVTTFGTFTHLIARADVPDDVVYG
                     LLEALVARRADLAAIVKAMDGVTPADLATPVGVPFHPGAERFWRDQGLLN"
     gene            2009..2317
                     /locus_tag="VGB88_09045"
     CDS             2009..2317
                     /locus_tag="VGB88_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647632.1"
                     /translation="MIRTIMIALISATVVLAGVDSAIAQAMGPCLPHAEAVAKLGTEY
                     GERQVGIGLGSRGSSVAELYVAESGTWTILITRTNGLSCIAASGDNWSSSPLLVDDAT
                     "
     gene            complement(2330..3304)
                     /locus_tag="VGB88_09050"
     CDS             complement(2330..3304)
                     /locus_tag="VGB88_09050"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008053594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEX9647633.1"
                     /translation="MKLTLDEMREAARAVYRVMPPTPQYAWPLLARLAGCEVWVKHEN
                     HTPIAAFKVRGGIVYVGELRARAPDCPGVVAATRGNHGQSIALAAARHGLRCVIVVPE
                     GNNPEKNDAMAAQGAALVVRGADFQQALEHAQALAEAEGLHMVPSFDRLLVMGVASYA
                     LELFEGAGPLDAVYVPIGLGSGICGVIAARDALGLTTEVIGVQAEGAPAYALSFAAGR
                     PVSTDRADTFADGVATRVPVAEAVEFINRGAARVVTVSDDDILDAQRALLRATHNLAE
                     PAGAAPLAALLSEHARMRARKVAVILSGGNADAANIRMLAATDARAHA"
     gene            3412..4287
                     /locus_tag="VGB88_09055"
     CDS             3412..4287
                     /locus_tag="VGB88_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647634.1"
                     /translation="MAFESFEARRIAASGCEVNLVVGGSGPPLLLLHGYPQTHFMWHK
                     VAPRLAEHYTVVASDLRGYGDSGRPADGDNHAGHCKRASAQDQVETMAALGFEQFMVA
                     GHDRGARVAHRMALDHPERVAKLSVLDIVPTRRVFADVSQALATAYYHWFFLIQPAPF
                     PETLIGNDPEYYLRWVMGRWGTAAGAITDEAFAEYLRCFARPETIHASCEDYRAAASI
                     DLDHDAADGERRIACPVQALWGARAVMHRMYDVVAVWREYAGDVRGQAVDCGHFLPEE
                     APEETWRALHAFFSS"
     gene            4366..5190
                     /locus_tag="VGB88_09060"
     CDS             4366..5190
                     /locus_tag="VGB88_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX9647635.1"
                     /translation="MSDERPDTGHEPAGGEDTEQGAKPAPRRGSFSRLRNYFLTGIIV
                     TAPIAITLWILFGIIKMIDNAVVPLLPPVYNPQTYLDRYFGITADIPGIGLVVMLFAL
                     TLIGFLAAGFFGRMVVRTGESLVNRMPVVRSVYGALKQIFETVLEARSRSFREVVLVE
                     YPRRGIWALGFITSTTEGEVQHVTAEKVVNVFLPTTPNPTSGFLLFVPRKDLVVLQMT
                     VEEGIKMVVSGGIITPPDRRPQRLQERPLVSSQGALAREGEDDEWRARALSPSAER"
     gene            complement(5168..>5978)
                     /locus_tag="VGB88_09065"
     CDS             complement(5168..>5978)
                     /locus_tag="VGB88_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9647636.1"
                     /translation="PRTLMLTAYGDMDVSRLTEKPPGRQPVTTAALPLSRLAEVVAAV
                     RRALDQGDRVYWVCPLVEESEALDLAAAEERFGALAERFGARVGLVHGRMKPAERDSM
                     MAAFAAGDITVLVATTVIEVGVDVAAATVMVVEHAERFGLAQLHQLRGRVGRGTRASS
                     CLLLYAPPLGETARARLDILRQTDDGFVIAEEDLRLRGAGELLGTRQSGVPEFRLADL
                     GAHAGLLGAARDDARLIVARDPGLETARGQSLKVLLYLFERDLAVTFLRSG"
BASE COUNT          905 a         2115 c         2137 g          821 t
ORIGIN      
        1 ccgctggtca ggcacgccac cttggccggg ccgccggggg tgtggccggt cagcgccagc
       61 gcgaacgaca tgaacagctc gccggtgccg acccgctcga gaaacgcgcc gaacatcacg
      121 aacagcacga cataggtcgc cgacacgccg agcgggatgc cgaacacgcc ttcggtggtc
      181 atgtacagct ggtcgaccag gtcggcgagc gggaagccct catgggcgag cccgaacggc
      241 agccacggcc cgagcatggc gtagccgagg aacagggcgg cggtcaccgg cagcgcccag
      301 ccgatcaccc gccgcgacgc ctcgagcacg agtgcgacca gggcgacgcc gagccacagg
      361 tcccagtcgg tcagctcgtc cacgtacggg aagcgggtga ccatgtactg gtagttgacc
      421 aggatgtggc cgatgccggc gaggccggcc gcgagcggcg cgccgtcgag cagcagccgc
      481 caggcggtcc cttggcgcgc cgggaacagc aggaagatca ggacgaaggc gaagccgaga
      541 tggaggccgc ggaagatcag cgcctcgggc ggcccgacca gcgccacgcc gaggtgaaag
      601 accgccatcg ccaccgcgat cgcggtggcc agccgatgca ccgtgagcca ccccatcgcc
      661 ggacgcggcg gcgacgcggc tagttcagca gcccctggtc gcgccagaag cgctcggcgc
      721 cggggtggaa cggcacgccc accggggtgg cgaggtctgc gggcgtcacc ccgtccatcg
      781 ccttgacgat cgccgccagg tcggcgcggc gcgcgaccag cgcctcgagc aggccgtaga
      841 ccacgtcgtc tggcacgtcg gcgcgggcga tcagatgggt gaacgtgccg aaggtggtca
      901 cctcctcggt ctgctcgggg taggtgttcg ccgggatggt acgcgggacg tattggggat
      961 tgtgggcctt cagcgcctcg agcttgtcgg cggggatgct gagcacgcgg atcggccggc
     1021 ccgcggcggt gtccatgacc gcgccggccg ggatcgtggt gatcacggtg aaggcgtcga
     1081 tgtgcccgtc cttgatcagc gacaccgcat ccgtgtagcc gacgtggttg acgctgctga
     1141 gatcgtcgta gccgaggccg tagacgtcga gcacgtgacg cgaggcctgc tcgccggtgt
     1201 ggccgcgcgg gccgaccgcg atcgcccgcc ccctcatgtc ggcgaccgtc tggatgccgc
     1261 tgtcggcggc gaccacgaac tggaacactt gcggatagag cgtcgccagg tggcggatgt
     1321 tggccgccgc cccgtcgaac ggcggcctgc cggcgatcgc gtcgaccgcc gcggtgacgt
     1381 tcgacagcgc gaactgggcc ttgccggcct cgaccgccat gacgttggtg atgccgccgc
     1441 cgggcatgac cctgaggcgc gagtcggcga gcgtgtcgcg cgccaggttg ctgatcgcgc
     1501 cgccgagcgg ataccacgag ccaccctgcg gcccggtcat gaaccgatag tcggcggcct
     1561 gcgcgccggc cgcgccggcg gcgagcgcga gcgacaccgc cagcgccgtg agccgcgccc
     1621 gggcggggcg gccggcagcc ggcgcgttgt gtgctgtcat gacatccctc ccgtcatcct
     1681 gccccgccgg cacgcaggct agcgcagccg ccgcgccggc gccaacggca atcccgccgc
     1741 ggcgcgaccg cacttcaggc cggcagaaca gccgagaccc ggaactgaaa cagacaaagg
     1801 cgaggcggcg ccgtcatccg ggtaaactat ctaaaaatga tcggacttta ccttttgatc
     1861 gttttggact gtcaacttgt cgtgagccga attgaacggg gaaaaccgaa tctcgccatt
     1921 gaaatcccta aggggtcgcc catcttcatt ggcgcaggga agacacaagg aggaggtcac
     1981 ggccaaccgc tggattacgg aggttgccat gatccgcact ataatgatcg cgctgatttc
     2041 ggcgactgtg gtcttggccg gcgtcgacag cgcaatagcc caggcgatgg gcccctgctt
     2101 gccgcacgcg gaagcggtgg cgaagctcgg caccgagtat ggcgaacgcc aggtcggcat
     2161 cgggctcggc tcgcggggca gctcggtcgc cgagctgtac gtcgccgaga gcggcacctg
     2221 gacgatcctg atcacgcgca ccaacggact gagctgcatc gccgccagcg gcgacaactg
     2281 gtcgtcaagc ccgctgctgg tcgacgacgc tacctgacct gcgcgcgcct cacgcgtgcg
     2341 ctcgcgcgtc cgttgcggcc agcatgcgga tgttggcggc gtcggcattg cctccggaca
     2401 agatgacggc gaccttgcgc gcgcgcatgc gcgcgtgctc gctgagcagc gccgccagcg
     2461 gcgccgcgcc cgcgggctcg gccaggttgt gggtggcgcg cagcaatgcg cgctgggcgt
     2521 cgaggatgtc gtcgtccgag accgtgacca cgcgcgcggc gccgcggttg atgaactcga
     2581 ccgcctccgc caccggcacc cgcgtcgcca cgccgtcggc gaaggtgtcg gcgcggtcgg
     2641 tcgacaccgg ccgcccggct gcgaacgaca gcgcgtaagc cggcgcgccc tcggcctgca
     2701 cgccgatcac ctcggtggtg agcccgagcg cgtcgcgcgc cgcgatcacc ccgcagatcc
     2761 ctgagccgag gcctatcggc acgtagaccg catcgagcgg cccggcgccc tcgaacagct
     2821 cgagcgcgta gctcgccaca cccatgacca agagccggtc gaaggacggc accatgtgca
     2881 ggccctcggc ctcggcgagc gcctgcgcgt gctccagcgc ctgctggaag tcggcgccgc
     2941 ggaccaccag tgccgcgccc tgcgccgcca tggcgtcgtt cttctcgggg ttgttgcctt
     3001 cgggcaccac gatcacgcag cgcaggccgt ggcgggcggc ggcgagcgcg atcgactggc
     3061 cgtgattgcc gcgggtcgcc gcgaccacgc ccgggcagtc gggcgcgcgc gcgcgcagct
     3121 cgccgacata gacgatgccg ccgcgcacct tgaacgccgc gatcggggtg tggttctcgt
     3181 gcttgaccca gacctcgcag ccggcaaggc gggcgagcag cggccaggca tattgggggg
     3241 tcggcggcat cacgcgatag accgcgcgcg cggcctcgcg catctcgtcg agcgtgagct
     3301 tcatcacgtc ctcccgtcgc cgcctagcct cgccgggcgc acgccgcccg acaacagaaa
     3361 gatgcacgcc ggcgccgcct tggcatagca tggccggagc gggaggcggc gatggcgttc
     3421 gagagcttcg aggcgagacg cattgcggcg agcggctgcg aggtcaacct ggtcgtcggc
     3481 ggctcgggcc cgccgctgct gctgctgcac ggctaccccc agacccattt catgtggcac
     3541 aaggtggctc ctcgcctcgc cgagcactac acggtggtgg cgagcgacct gcgcggctac
     3601 ggcgacagcg gccgtcccgc cgacggcgac aaccatgccg gccattgcaa gcgcgccagc
     3661 gcccaggacc aggtcgagac gatggcggcg ctcggcttcg agcagttcat ggtcgccggc
     3721 cacgaccgtg gcgcccgcgt cgcgcaccgc atggcgctcg accaccccga gcgggtggcg
     3781 aagctctccg tgctcgacat cgtccccacc cggcgcgtgt tcgcggacgt gagccaggcg
     3841 ctcgcgaccg cctattacca ctggttcttc ctgatccagc cggcgccgtt ccccgagacc
     3901 ctgatcggca acgaccccga gtattacctg cgctgggtga tggggcgctg gggcacggcc
     3961 gcgggcgcga tcaccgacga ggcgttcgcc gagtacctgc gctgcttcgc ccggcccgaa
     4021 accatccacg cctcgtgcga ggactaccgg gcggcggcga gcatcgacct cgaccacgac
     4081 gccgccgacg gcgagcgcag gatcgcctgc ccggttcagg cgctgtgggg cgcccgcgcc
     4141 gtgatgcacc ggatgtacga cgtggtcgcg gtgtggcgcg agtacgccgg cgacgtgcgg
     4201 ggccaggcgg tcgattgcgg ccacttcctg cccgaggagg ccccggagga gacctggcgg
     4261 gcgctgcacg ccttcttctc gtcctgagcg gctggtgcgg cgctcccttg cgtgagcggt
     4321 cgcgatgccg gtatcactgg aggcggggcg cctggagttg agaccatgag cgatgagcgg
     4381 ccggacaccg ggcacgagcc cgcgggcggc gaggacacgg agcagggcgc caagcccgcg
     4441 cccaggcgcg gatcgttctc gcgcctgcgc aactacttcc tgaccggcat catcgtcacc
     4501 gcgccgatcg cgatcacgct gtggatcctg ttcgggatca tcaagatgat cgacaacgcg
     4561 gtggtgccgc tgctgccgcc ggtctacaac ccgcaaacct atctcgatcg ctatttcggc
     4621 atcaccgccg acatcccggg aatcggcctg gtggtgatgc tgttcgcgct gaccctgatc
     4681 ggcttcctcg ccgccggctt cttcggccgc atggtggtgc gcaccggcga gagcctggtc
     4741 aaccgcatgc cggtggtgcg cagcgtctac ggcgcgctca agcagatctt cgagaccgtg
     4801 ctcgaggcgc gatcgcgctc gttccgcgag gtggtgctgg tcgagtaccc gcgccgcggc
     4861 atctgggcgc tcggcttcat caccagcacc accgagggcg aagtccagca cgtcaccgcc
     4921 gagaaggtgg tcaacgtgtt cctgccgacc acgcccaacc cgacctcggg atttctcctg
     4981 ttcgtgccgc gcaaggacct ggtggtgctg cagatgaccg tcgaggaggg tatcaagatg
     5041 gtcgtctcgg gcggcatcat caccccgccc gaccggcgcc cgcagcggtt gcaggagcgc
     5101 ccgctggtct cgtcccaggg cgcgctggcg cgcgagggcg aggacgacga gtggcgcgcg
     5161 cgcgcgctca gcccgagcgc agaaaggtga ccgcgaggtc gcgctcgaac aggtaaagca
     5221 gcaccttgag gctctggccg cgggccgtct cgagccccgg gtccctggcg acgatcaggc
     5281 gggcgtcgtc gcgcgccgcg ccgagcaggc cggcgtgcgc gccgaggtcg gcgaggcgga
     5341 actcgggcac cccgctctgg cgcgtgccca gcagctcgcc ggcgccgcgc aggcgcaagt
     5401 cctcctcggc gatgacgaag ccgtcgtcgg tctggcgcag gatgtcgagc cgcgcgcgcg
     5461 cggtctcgcc cagcggcggc gcgtacagca gcaggcagct cgatgcgcgc gtgccgcgtc
     5521 cgacccgccc gcgcagctgg tgcagctggg cgagcccgaa gcgctcggcg tgctcgacca
     5581 ccatcaccgt cgccgcggcc acatcgacac cgacctcgat caccgtggtc gcgaccagca
     5641 ccgtaatgtc gccggcggcg aaagcggcca tcatcgaatc gcgctcggcc ggcttcatgc
     5701 gcccgtgaac caggccgacg cgcgcgccga agcgttccgc gagcgcgccg aagcgttcct
     5761 cggcggcggc gaggtcgagc gcctcggact cctcgaccag cgggcatacc cagtagaccc
     5821 ggtcgccctg gtcgagggcg cggcgcaccg ccgccaccac ctccgcgagg cgcgacagcg
     5881 gcaacgccgc ggtggtcacc ggctggcggc cgggcggctt ctcggtcagg cgcgatacgt
     5941 ccatgtcgcc ataggcggtc agcatcaggg tgcgcggg
//
LOCUS       DASWHU010000246         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2718126, whole genome shotgun sequence.
ACCESSION   DASWHU010000246 DASWHU010000000
VERSION     DASWHU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718126"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1623)
                     /locus_tag="VGB88_09070"
     CDS             complement(175..1623)
                     /locus_tag="VGB88_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647637.1"
                     /translation="MARMQKFISVGKEMPDKRDAAVRRHDFDEIYQDYARDKAAEQSS
                     RCSQCGVPFCQIHCPLHNNIPDWLLLCAQGRLEEAYEVSSATNNMPEVCGRICPQDRL
                     CEGNCVIEQAGHGTVTIGAVERYITDTAWERGWVRPPKPVRELDHSVGVIGAGPAGLA
                     AAEQLRRKGLQVTVYDRYDRVGGLLVYGIPSFKLEKHLVARRHEMLAAGGIDFELEFE
                     IGRDASFAELRERHDAVLVASGVYKGRDIMCPGVGLDNIVPALDYLIASNRKGLGDEV
                     PAFDSGALDAAGKNVVVVGGGDTAMDCVRTAVRQGAKSVKCLYRRDRANMPGSQREVQ
                     HAEEEGVEFVWLSQPEAFIGEGAVDGVRVVQMRLGPPDPTGRQKPIPIEGAHYTLEAD
                     LVIKALGFDPEDLPRLFDEPGLEVTRWGTVKCDFRTMMTNQYGVFAAGDIVRGASLVV
                     WAIRDGRDAAAAIIRHVTAHAAAGAARAVAAE"
     gene            1975..2772
                     /locus_tag="VGB88_09075"
     CDS             1975..2772
                     /locus_tag="VGB88_09075"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9647638.1"
                     /translation="MITLHLLVVALVQGITEFLPISSSAHLVLIPIATGWPDQGIAID
                     IAAHVGSLGAVFVYFRRDIAALAAAALAVLGGRVHPAGRLLAWLIVSTIPALVAGFAL
                     ADAAGTAFRDIAVIAWTMTLGAVLLYLADRYGGVARPLDDMRARDALWIGLAQVLALV
                     PGTSRAGITMTMARVLGITRADAARFSMLLSIPVIAGAGTMDGLALVARGDAPLIGDA
                     LIAAALSFVAALAAIAVLMRWLERSSFTPLVVYRLALGLVLLAIVYL"
     gene            complement(2786..3748)
                     /locus_tag="VGB88_09080"
     CDS             complement(2786..3748)
                     /locus_tag="VGB88_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX9647639.1"
                     /translation="MAVAQATVFGGSGFLGRYVVKRLAEAGARVLVAVRRPELAGFLR
                     PMGDVGQIMPIRVDLADANAVAAAVEGSDAAVNLVGILVERGAQRFAAVHAQGAERVA
                     KAAQAHGVRRLVHVSAIGADRAAPSAYGRSKAAGEAAVRAAFEGATIVRPSVVFGPED
                     DFFNRLAALARMSPVLPLIDGGHTRFQPVYVGDVADAIMRILDDDRTAGRTFELGGPR
                     IYTFRELIAYVVEQTGRRRLLVPVPSALMRLKAWFLEFLPKPPLTRDQVAQLAADTVV
                     HTDPEAGVATFADLAITPAAVEDVVPEYLARYRRGGGARPGREA"
     gene            4065..4151
                     /locus_tag="VGB88_09085"
     tRNA            4065..4151
                     /locus_tag="VGB88_09085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4099..4101,aa:Leu,seq:cag)
     gene            4211..>4629
                     /locus_tag="VGB88_09090"
     CDS             4211..>4629
                     /locus_tag="VGB88_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX9647640.1"
                     /translation="MTVHLHRYDLPAEVELGATIAVDTETAGLSLVRDRLCAVQLSAG
                     DGDAHVVHFPTPDYDAANLKRVLADSSATKLFHFARFDLAVLSRALGVVSAPVYCTKV
                     ASRLVRTYSDRHGLKDLCQELLGIELSKQQQSSDWAAP"
BASE COUNT          621 a         1707 c         1609 g          692 t
ORIGIN      
        1 cgcggtccga gccacatgcc gatggtgccg agatcgtcgt cgagcgtctc gggcgcgtgc
       61 agcgtccgct gccagcggct ggaggtctcg acgtgctctg cgagggtcat gggttacacc
      121 caaacaagac aagtctagca cccgcgcgcc cgcgccgata cgccgtcacc gccgtcactc
      181 cgccgccacc gcgcgcgcgg cgccggccgc ggcgtgcgcc gtgacgtgcc ggatgatcgc
      241 cgccgcggcg tcgcgcccgt cgcggatcgc ccacaccacc agcgaggcgc cgcgcacgat
      301 gtcgccggcg gcgaacaccc cgtactggtt ggtcatcatg gtccggaagt cgcacttgac
      361 ggtgccccag cgggtgacct cgagcccggg ctcgtcgaac agccgcggca ggtcctcggg
      421 gtcgaagccg agcgccttga tcaccagatc cgcttccagc gtgtagtgcg cgccctcgat
      481 cgggatcggc ttctggcgcc cggtcggatc gggcgggccg aggcgcatct gcaccacgcg
      541 cacgccgtcg accgcgccct cgccgatgaa cgcctcgggc tgggacagcc acacgaactc
      601 gaccccttcc tcctcggcgt gctgcacctc gcgctgcgag ccgggcatgt tggcgcggtc
      661 gcgccggtat aggcacttga ccgacttcgc gccctggcgc accgcggtgc gcacgcagtc
      721 catcgcggtg tcgccgccgc cgaccaccac cacgttcttg ccggcggcat cgagcgcgcc
      781 ggagtcgaac gcgggcacct cgtcgccgag gcccttgcgg ttgctcgcga tcaggtagtc
      841 gagcgccggc acgatgttgt cgaggccgac ccccgggcac atgatgtcgc gccccttgta
      901 gacgccgctc gcgaccagca ccgcatcgtg gcgctcgcgc agctcggcga agctcgcgtc
      961 gcggccgatc tcgaactcga gctcgaagtc gatgccgccc gccgccagca tctcgtggcg
     1021 gcgcgcgacc aggtgcttct cgagcttgaa gctggggatg ccgtagacca gcaggccgcc
     1081 gacccggtcg tagcggtcgt agaccgtgac ctgaaggccc ttgcggcgga gctgctcggc
     1141 ggcggcgaga ccggccgggc cggcgccgat gacgcccacc gaatggtcga gctcgcgcac
     1201 cggcttgggc ggcctgaccc agccccgctc ccaggcggtg tcggtgatgt agcgctcgac
     1261 cgcgccgatg gtgacggtgc cgtggccggc ctgctcgatc acgcagttgc cctcgcacag
     1321 ccggtcctgc gggcagatgc gcccgcacac ctcgggcatg ttgttggtcg ccgaggacac
     1381 ctcgtaggcc tcctcgagcc gcccctgcgc gcacagcagc agccagtcgg ggatgttgtt
     1441 gtgcagcggg cagtggatct ggcagaacgg caccccgcac tgcgagcagc gcgaggactg
     1501 ctcggccgcc ttgtcgcgcg catagtcctg atagatctcg tcgaagtcgt gacgccggac
     1561 ggcggcgtcg cgcttgtcgg gcatctcctt gcccaccgag atgaacttct gcatgcgtgc
     1621 catgcgcttc caccgagttg cggcgcgcga cccccgctgt gcgagacggg ccgcggccgt
     1681 ccgtccgcgt gcggggccgg tcgccaccgg agtccgggtg gccagggccg tccccgcacg
     1741 gccgagatca gatgataagg gcgaccgcgg ccgaaggcca gcgattttcc gatcttatga
     1801 caatatagct gtcgtataat ttcacgcggc ctgccgccaa gtggcagaat cgagcgaaat
     1861 ctcagcagcg ggcaggacaa ttagtctcaa ttcgggacta tcttcccgct tccgcacctg
     1921 cgcccgcccg gatatgccag ggccaaccga ttcggttggc acggggacgg cggcgtgatc
     1981 accctgcacc tgttggtcgt cgccctggtc cagggcatca ccgagttcct gccgatcagc
     2041 tcgtcggcgc atctggtcct gatcccgatc gccaccggct ggcccgacca gggcatcgcg
     2101 atcgacatcg ccgcccatgt cggcagcctg ggcgcggtgt tcgtctactt ccggcgcgac
     2161 atcgccgcgc tggcggcggc agcgctcgcg gtgctgggcg ggcgggtgca tccggccggc
     2221 cggctgctcg cctggctgat cgtcagcacc atcccggcgc tggtcgccgg cttcgccctg
     2281 gcggacgccg cgggcacggc gttccgcgac attgccgtga tcgcctggac gatgacgctc
     2341 ggcgcggtcc tgttgtatct cgccgaccgc tacggcgggg tcgcgcgccc gctcgacgac
     2401 atgcgcgcgc gcgatgcgct gtggatcggg ctcgcccagg tgctcgccct ggtcccgggc
     2461 accagccgcg ccggcatcac catgaccatg gcgcgcgttc tcggcatcac ccgcgccgac
     2521 gccgcccggt tctcgatgct gctgtcgatc ccggtgatcg cgggcgccgg caccatggac
     2581 gggctggcgc tggttgcccg cggcgacgcg ccgctgatcg gcgatgcact gattgccgcc
     2641 gcgctgtcgt tcgtagccgc cttggccgcg atcgccgtgc tgatgcgctg gctcgagcga
     2701 tcgagcttca cgccgctggt ggtctaccgg ctggcgctcg ggctggtgct gctggcgatc
     2761 gtgtatctgt agcgcgaccg ccggctcacg cctcgcggcc cggccgcgcc ccgccgccgc
     2821 gccggtaccg ggccaggtac tcgggcacca cgtcctcgac cgcggccggc gtgatggcga
     2881 gatcggcgaa ggtggcgacg ccggcctcgg ggtcggtatg gaccacggtg tcggcggcga
     2941 gctgcgccac ctggtcgcgg gtcagcggcg gcttgggcag gaactcgaga aaccaggcct
     3001 tgaggcgcat cagcgcgctc ggcaccggca ccagcagccg gcggcggccg gtctgctcga
     3061 cgacataggc gatcagctcg cgaaacgtat agatgcgcgg gccgccgagc tcgaaggtgc
     3121 gcccggcggt ccggtcatcg tcgaggatgc gcatgatcgc gtcggcgacg tcaccgacat
     3181 agaccggctg gaagcgggta tggccgccgt cgatcagcgg cagcaccggc gacatgcggg
     3241 ccagcgccgc caggcggttg aagaagtcgt cctcgggccc gaagaccacg ctcggacgga
     3301 cgatggtcgc gccctcgaac gccgcccgca cggccgcctc gccggctgcc ttgctgcgcc
     3361 cataggcgct gggcgcggcg cggtcggcgc cgatcgccga gacgtgcacc agccggcgca
     3421 cgccgtgggc ctgggccgcc ttggcgaccc tctcggcgcc ctgggcgtga accgcggcga
     3481 agcgctgggc gccgcgctcg accaggatgc cgaccaggtt gaccgccgcg tccgagcctt
     3541 cgaccgcggc cgccaccgcg ttggcgtcgg cgaggtcgac ccggatcggc atgatctggc
     3601 cgacgtcgcc catcgggcgc aggaagcccg ccagctccgg ccggcgcacc gccaccagca
     3661 cccgcgcgcc cgcctcggcc agccgcttga ccacatagcg gccgagaaaa cccgagcccc
     3721 cgaacacggt tgcctgcgcc accgccatgc cgctggatcc cgcttcctcg cccgtccctt
     3781 cccttgtccc cgggtgtgcg ggaagcgggc ctgcgatacc aattgtcgac aagatcgcgc
     3841 cgtttcattg ccgcagcgcc tgagcgccgc gcgggcgtcg gcgacgccgc gcccgtgccc
     3901 tcgcatgaaa cggctcgatg ttgtcgaccg gcaacattct cgcccatcgg gacgccccgg
     3961 cgccagcaca aagtgcgttg acacgggccg ccgcacgggg atagtcacaa cccccgcaac
     4021 cgccggcccg ggccgggttt tcccccggcg gggcggcccc tttagcccag gtggcggaat
     4081 tggcagacgc gctggcttca ggtgccagtg accgcaaggt cgtggaagtt cgagtcttct
     4141 cctgggcacc acaccggcgc gcgcgcgcca gcggcccgtg cccgggagcg ggcgccggcg
     4201 cgcggcggcg atgaccgtcc atctccaccg ctacgacctg cccgccgagg tcgagctcgg
     4261 cgccacgatc gcggtcgaca ccgagaccgc gggcctcagc ctggtccgcg accggctgtg
     4321 cgcggtgcag ctgtcggcgg gcgacggcga cgcgcatgtg gtccatttcc ccacccccga
     4381 ctacgacgcg gccaacctga agcgggtgct cgcggattcg tcggcgacca agctgttcca
     4441 tttcgcccgt ttcgacctgg cggtgctcag ccgcgcgctc ggcgtggtct ctgccccggt
     4501 ctactgcacc aaggtcgcct cgcggctggt gcgcacctac agcgaccgcc acgggctcaa
     4561 ggacctgtgc caggagctgt tgggcatcga gctgtcgaag cagcagcaga gctcggactg
     4621 ggcggcgcc
//
LOCUS       DASWHU010000247         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_864162, whole genome shotgun sequence.
ACCESSION   DASWHU010000247 DASWHU010000000
VERSION     DASWHU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864162"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VGB88_09095"
     CDS             complement(<1..1143)
                     /locus_tag="VGB88_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX9647641.1"
                     /translation="MDARPDIAAYPALEARFRRLALLGDALGLLAWDRRVMMPAGGAE
                     ARAAEITELGLVRHRMLAEPALADLLDQAEAAAGDLDSWQRANLGHMRRTWRHATAVP
                     ADLYEAATRAELACEMAWRRARPANDFAALAPALAEVVRLKRETAAAKGAALGCAPYD
                     ALLDQFSPGLRAAQYAPLFDELAAFLPGFIDRVIAAQAREPPVLRPSGPFPIARQRRL
                     ARELMARVGFPFDSGRLDESDHPFSGGTPEDLRVTTRYDEADFATALMAVLHETGHAL
                     YQRNLPAAWRHQPVGEALGIAVHESQSLLIEMQACRGRAFLAFAAPRMCAAFAGDGPA
                     WSADNIFRLYTRVERGLIRVDADEVTYPAHILLRTRLETALIAGTLR"
     gene            complement(1127..>1952)
                     /locus_tag="VGB88_09100"
     CDS             complement(1127..>1952)
                     /locus_tag="VGB88_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9647642.1"
                     /translation="LWLPTPAKLAAIPGVDYSFGQVMAEWTHRLNAAAVVTPLVVVLV
                     ALAWRLRRERTDLMATMAWALAVLAAQAVLGGFTVLDRNSPWSVAVHLTAALLLLALV
                     LRAHMAARGAVGASPVADVRHFANAALLLTLITVAGGAMVAKSGATLACASWPLCDGA
                     LVPDLGDALVRTNFGHRLLALGTAAAVFALWRVARRLDGGARGPLVRAANMALATLAL
                     QIAVGALVVPAFAGGSLALQVAAGTVHQAVGVVLFATLAVMLWRAQAVGSAGGRAA"
BASE COUNT          287 a          728 c          737 g          200 t
ORIGIN      
        1 gcggagcgtg cccgcgatca gcgccgtctc caggcgcgtg cgcaaaagga tgtgcgcggg
       61 ataggtgacc tcgtcggcat cgacccggat caagccgcgc tcgacccggg tatacaggcg
      121 gaagatgttg tcggcgctcc acgccggccc gtcgccggcg aaggcggcgc acatgcgcgg
      181 tgcggcgaag gcgaggaagg cgcggccgcg gcaggcctgc atctcgatca acagcgattg
      241 gctctcgtgc acggcgatgc cgagcgcctc gccgaccggc tggtggcgcc aggcggcggg
      301 caggttgcgc tggtagagcg cgtggccggt ctcatgcagg accgccatca gcgcggtggc
      361 gaagtcggcc tcgtcgtagc gcgtggtcac gcgcaggtcc tcaggcgtgc cgccggagaa
      421 cggatggtcg ctctcgtcga gccggccgct gtcgaacggg aagccgaccc gggccatcag
      481 ctcgcgcgcg aggcggcgct ggcgcgcaat cgggaacggt ccggagggcc gcagcaccgg
      541 cggctcgcgc gcttgcgccg caatcacccg gtcgatgaag ccgggcagga acgccgccag
      601 ctcgtcgaac aacggcgcgt attgggccgc gcgcaggccg gggctgaact ggtcgagcag
      661 cgcgtcgtag ggcgcgcagc cgagcgccgc gcccttggcg gcggcggtct cgcgcttcag
      721 ccgcacgacc tcggccagcg ccggcgcgag cgcggcgaag tcgttggccg ggcgcgcccg
      781 ccgccacgcc atctcgcagg cgagctcggc ccgcgtcgcc gcctcgtaga ggtccgccgg
      841 caccgcggtg gcgtggcgcc aggtgcggcg catgtggccg agattggcgc gctgccagga
      901 atcgaggtcg ccggccgccg cctccgcctg gtcgagcagg tctgcgagcg cgggctcggc
      961 cagcatccgg tggcggacca ggccgagctc ggtgatctcg gccgcgcgcg cctcggcccc
     1021 gcccgcgggc atcatcaccc gccggtccca ggccagcagc ccgagcgcgt cgccgagcag
     1081 cgcgaggcgc cggaagcgcg cctcgagcgc cgggtaggcg gcgatgtcag gccgcgcgtc
     1141 caccggcgct gccgaccgct tgcgcgcgcc acagcatcac cgccaaggtc gcgaacagga
     1201 ccacgccgac cgcctggtgc acggtgccgg cggccacctg aagcgccagc gagccgccag
     1261 cgaaggccgg caccaccagc gcgccgaccg cgatctggag cgcgagcgtc gccaacgcca
     1321 tgttggcggc acgcaccagc ggcccgcgcg cgccgccgtc gaggcgccgc gcgacccgcc
     1381 acagggcgaa caccgccgcc gccgtgccga gcgcgagcag gcggtggccg aaattggtcc
     1441 gcaccagcgc atcgccgagg tcgggaacca gcgcgccgtc gcacagcggc caggaggcgc
     1501 aggcgagcgt cgccccggac ttcgccacca tcgccccgcc ggcgacggtg atcagggtga
     1561 gcagcagcgc ggcattggcg aagtgcctga catcggccac cggcgaggcg ccgacggcgc
     1621 cgcgcgcagc catgtgggca cgcagcacca gcgccagcag cagcaacgcc gcggtcaggt
     1681 gaaccgccac cgaccacggg ctgttgcggt cgagcacggt gaagccgccg agcaccgcct
     1741 gcgccgccag caccgccagc gcccaggcca tggtcgccat caggtccgtg cgctcgcggc
     1801 gcagccgcca agccagcgcg accagcacca cgaccagcgg cgtgaccacc gcggcggcat
     1861 tgagccggtg ggtccactcg gccatcacct ggccgaagct gtaatcgacg ccgggaatcg
     1921 cggcgagctt ggccggggtc ggcagccaca gg
//
LOCUS       DASWHU010000248         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_506606, whole genome shotgun sequence.
ACCESSION   DASWHU010000248 DASWHU010000000
VERSION     DASWHU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506606"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VGB88_09105"
     CDS             complement(<1..919)
                     /locus_tag="VGB88_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647643.1"
                     /translation="MTDFESVRIALRALRVNTLRSALTMLGIIIGVAAVVTMVSVGRG
                     AHVQVAEQIRSLGTNLLTVSPGAARQGGVRLESGSRRNLTESDARAIRAEVAAVEVAA
                     PAVRGKAQIVQGNRNWSTSVFGTEPDYFLARDWGIGAGRMFTVQDVASGAKVALIGDT
                     VAEALFGGADPLGRTVRIANVPFTVAGVLAKKGPTGSGRDQDDAVFVPISAAKLRLQL
                     GSAVDRDAVDYIMVKLASVDAMATAQADIRALLRQRHRLRADADDDFAVYDPTAEMQA
                     YQEATRTFTLLVGAIASVSLLVGGISIMNI"
     gene            complement(916..>1508)
                     /locus_tag="VGB88_09110"
     CDS             complement(916..>1508)
                     /locus_tag="VGB88_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647644.1"
                     /translation="MHLLGLLDRPSSGGYWFEGRDVGRLGGDERAAVRNRKVGFVFQS
                     FNLLARATAAENVELPLVYAGVRRAERRRRAEAALEAVALGPRRRHWPHQLSGGEQQR
                     VAVARAIVNDPSLVLADEPTGSLDSRTGLDILALLDSLHQAGRTVVLVTHEPQVARHA
                     GRIVTLRDGRVISDERVPVPLDAARERLAVSAEVMP"
BASE COUNT          204 a          536 c          557 g          211 t
ORIGIN      
        1 tgatgttcat gatgctgatg ccgccgacca gcagcgacac gctggcgatc gcgccgacca
       61 ggagcgtgaa ggtgcgcgtc gcctcctggt aggcctgcat ctcggcggtc gggtcgtaga
      121 ctgcgaagtc gtcgtcggca tcggcgcgca ggcgatggcg ctggcgcagc agcgcgcgga
      181 tgtccgcttg cgccgtcgcc atggcgtcga cgctggcgag cttgaccatg atgtagtcga
      241 ccgcgtcgcg gtcgaccgcg ctgccaagct ggaggcggag cttggccgcc gagatcggca
      301 cgaacacggc gtcgtcctgg tcgcggccgc tgccggtcgg tcccttcttc gccagcacgc
      361 cggccacggt gaaggggacg ttggcgatgc ggacggtgcg cccaaggggg tcggcgcccc
      421 cgaacagtgc ttcggccacc gtgtcgccga tcagtgccac cttggccccg gaggccacgt
      481 cttggacggt gaacatgcgc cccgcgccga tgccccagtc gcgggcgagg aagtagtccg
      541 gctcggtgcc gaacacggag gtgctccagt tgcgattgcc ttggacgatc tgcgccttgc
      601 cgcgcacggc gggcgccgcg acctcgaccg cggcgacctc ggcgcggatc gcgcgcgcgt
      661 ccgactcggt caggttgcgg cgactgccgc tttccaggcg cacgccgccc tggcgcgccg
      721 cgcccggcga gaccgtgagc aggttcgtcc ccaggctcct gatctgctcg gcgacctgga
      781 cgtgggcgcc gcggccgacg ctgaccatgg tgaccacggc ggcgacgccg atgatgatgc
      841 cgagcatggt gagcgcgctg cgcagcgtgt tgacccgaag cgcgcggagc gcgatgcgca
      901 cactctcgaa gtcggtcacg gcattacctc cgcgcttacg gcgaggcgct ctcgcgccgc
      961 atcgagcggc accggcacgc gctcgtcgct gatcacgcgg ccgtcgcgca gggtgacgat
     1021 gcgcccggcg tggcgcgcca cctgcggctc gtgggtcacc agcacgacgg tcctcccggc
     1081 ctggtgcagc gagtcaagga gcgccaggat gtcgagcccg gtgcgcgaat ccagcgagcc
     1141 ggtcggctcg tcggcgagca ccagggacgg gtcgttgacg atcgcgcggg ccacggccac
     1201 gcgctgctgc tcgcccccgg aaagctggtg cggccagtgc cggcgccgcg gcccgagcgc
     1261 caccgcctcg agcgcggcct cagcgcggcg gcggcgctcg gcgcgcctga cgccggcata
     1321 gaccagcggc agctctacgt tttccgccgc cgtggcgcgg gcgagcaagt tgaacgactg
     1381 gaacacgaag ccgaccttgc ggttgcggac ggcggcgcgc tcgtcgccgc cgagccggcc
     1441 cacgtcgcgg ccctcgaacc agtagccgcc tgagctcggc cggtcgagaa gcccgagcag
     1501 gtgcatca
//
LOCUS       DASWHU010000249         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3102056, whole genome shotgun sequence.
ACCESSION   DASWHU010000249 DASWHU010000000
VERSION     DASWHU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3102056"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VGB88_09115"
     CDS             complement(<1..427)
                     /locus_tag="VGB88_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9647645.1"
                     /translation="MNDFRPVLFVIGLLLVILAVAMVLPAIADLAAGNPDWQVFLLSS
                     AVTLFAGVSLVLAFRVPRFRVDIRQTFVLTTLSWVVLTAFAALPFAFSTLGLSFTDAY
                     FEAMSGLTTTGSTVIVGLDAAPPGLLFWRSLLQWLGGIGI"
     gene            complement(609..1052)
                     /locus_tag="VGB88_09120"
     CDS             complement(609..1052)
                     /locus_tag="VGB88_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEX9647646.1"
                     /translation="MGLVERLDDYGKPAWIAVMILGFIIFWPLGLLILAYLIWSRRMG
                     CWKHGAPGRWHYADGDHRRRDRERWRGGRGRAPSTGNRAFDEYREETLRRLEDEQGEF
                     EQFLERLRHAKDKMEFDQFMDERRRRPPAGDDRAAPEGPVPQPNG"
     gene            complement(1111..>1611)
                     /locus_tag="VGB88_09125"
     CDS             complement(1111..>1611)
                     /locus_tag="VGB88_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX9647647.1"
                     /translation="VSPAAPYRHFRDREALMAEVARRGFERFADALLRAWNDAEPDPY
                     TAFKNMGRAYLTFARAEPAYYAAMFEAGLPPDASPELSRAGDAAFAVVRRAAETLIAT
                     LPKDSRPPALMMSLHIWALSHGIASLFARGDAARRKVPMPPEDLLEAGVLIYLRGLGI
                     DPGHAS"
BASE COUNT          274 a          544 c          583 g          210 t
ORIGIN      
        1 tgatgccgat gccgccgagc cactgcagca gcgagcgcca gaacagcagc ccgggcggcg
       61 ccgcgtcgag gccgacgatc accgtcgagc cggtggtggt gagccccgac atggcctcga
      121 aataggcgtc ggtgaatgac agaccaaggg tcgagaacgc gaacggcagc gcagcgaagg
      181 cggtgagcac gacccagctc aaggtggtca gcacgaaggt ctggcggatg tcgacccgga
      241 agcgcggcac gcgaaaggcg agcaccaggc tcaccccggc gaacaaggtg accgccgacg
      301 acagcaggaa cacctgccag tcggggttgc cggcggcgag gtcggcgatc gccggcagca
      361 ccatggccac cgccaggatc accagcagca ggccgatcac gaacaggacg ggacgaaaat
      421 cattcaccgc agacgccgcg gtcgagggaa ccgggaagtg tcggattggc gttgtcaggc
      481 ttgacccgat attaccacga attggcggcc tggagggatg ggccgcgcct ggccggcgag
      541 cggacgcgca caaagcgagg gcgccgggca atcacgtgcc cggcgcccgc atcgtcgtcg
      601 ggggcggctc agccgttcgg ctgcgggacc ggcccttcgg gcgcggcgcg atcgtcgccg
      661 gccggcggcc gacggcggcg ctcgtccatg aactggtcga actccatctt gtccttggcg
      721 tggcgcaggc gctcgaggaa ctgctcgaac tcgccctgct cgtcctcgag gcggcgcagc
      781 gtctcctcgc ggtactcgtc gaaggcgcga ttgcctgtcg agggcgcgcg ccctcggccg
      841 ccgcgccagc gctcgcggtc ccggcgccga tggtcgccat cggcgtagtg ccagcggccg
      901 ggggcgccgt gtttccaaca tcccatgcgt cggctccata tcagatacgc caggatcagc
      961 aggccgaggg gccagaagat gatgaaaccg aggatcatca cggcgatcca ggccggcttg
     1021 ccgtaatcgt ccagtcgctc gacgagtccc atcagaccct cgcgtgaatg ttaataacat
     1081 tcacatagct agccattgcc gacccgcctg tcaagaggcg tggccggggt ctatgccgag
     1141 gccccggaga tagatcagga cgccggcctc gagcaggtcc tcgggcggca tcggcacctt
     1201 gcgccgggcc gcgtcgccgc gcgcgaacag cgaggcgatg ccgtgcgaca gcgcccagat
     1261 gtgcaggctc atcatcagcg ccggcggccg gctgtccttg ggcaaggtcg cgatcagcgt
     1321 ctccgcggcg cggcgcacca ccgcgaacgc cgcgtcgccg gcgcgcgaca gctcggggct
     1381 cgcgtccggc ggcagcccgg cctcgaacat cgccgcgtag taggccggct cggcgcgggc
     1441 gaaggtgaga taggcccggc ccatgttctt gaacgcggtg taggggtcgg gctcggcatc
     1501 gttccaggcg cgcagcagcg cgtcggcgaa gcgctcgaag ccgcgccgtg cgacctcggc
     1561 catcagcgcc tcgcggtcgc ggaagtgccg gtagggcgcc gccgggctga c
//
LOCUS       DASWHU010000250         7290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_865839, whole genome shotgun sequence.
ACCESSION   DASWHU010000250 DASWHU010000000
VERSION     DASWHU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865839"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VGB88_09130"
     CDS             complement(<1..919)
                     /locus_tag="VGB88_09130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9647648.1"
                     /translation="MDVAPAQLDSYYAARLGAVAARAPLDGRLDAEVCIVGGGLAGVS
                     CALELARRGVGVALIEARRLGWGASGRNGGQALIGYSPGMAPIARQFAPAEARRLWDM
                     TIDGLGAIVDRIARLRIACDLGAGNLVAATRRASLAAQAAWAETARRAYGFDGYRLLD
                     RGDVRREVASERYHGGLIEATSRHLDPLAYLAGLADAAQGQGARLYEDSPAIRVAFAD
                     QPQVWTARGTVRAKSLVLAGNAHLGRLVPALAARVMPVASYVCATEPLGEARARALIP
                     NRVAVCDDRAVLDYYRLTGDGRLLFGGGAS"
     gene            991..1446
                     /locus_tag="VGB88_09135"
     CDS             991..1446
                     /locus_tag="VGB88_09135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9647649.1"
                     /translation="MPDSRDPTGAATVEFDPARPASFPFWTRDVLRWGDTDALGHINN
                     VEFARFFESGRIAYLSAAGVRGIDADDFLLAHLAIDFHAQMHYPGEVRTGTRVIRIGR
                     SSVRFAQALFQDDLCTASGVAVAVMAGRDTGQAKPVPEDMRARLLDPGA"
     gene            1478..3352
                     /locus_tag="VGB88_09140"
     CDS             1478..3352
                     /locus_tag="VGB88_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9647650.1"
                     /translation="MAGEAVLATQPWQMWAVFVLIALAVAALVAERVAIEITAALVLA
                     ALLMLFQLAPLVEESGADRLDSSRLLSGFANPGLITVVALLVIGQALIQTGALEGVAN
                     LLYRVSRGRLMRAMALSLVPVAALSSVLNNTPVVVIFIPIMSALAERLRISTSRVLMP
                     LSFAAILGGMTTLIGSSTNLLVAGTIRDLGQPAIGFFDFAVPGLVLAGVGLAYLLFVA
                     PWLMPDRASLAGRLVGAGRQFIAQVEVQAGSPLIGERPIAGMFRKLPDITVRTVQRGE
                     HAFLPPFDDVTLQAGDVIVAAATRKALTGALAKHAGALHPSLTHRQITVAPDQHPDAD
                     RPWRGGEQALAEAMVTPGSRMIGQTLEQFGFRHQFRCIVVGVQRRSRMLRQRLTDIRL
                     EAGDVLLIQGRPEDVRALRANRDVVLIEWTEETLPKYVHARRAGAIFAAVVVLAATGA
                     VPIVVAAVVGAAAMLIAGCLNVRQAGRAIDRAVVMTVAAALALAAALQETGGATFLAR
                     QLIAALGDSGPGVMLSALFLLIALVTNLLTNNAAAVLFTPIAVSLALELGVDPTAFIH
                     AVIFAASCSFASPIGYQTNLMVMAPGHYRFADYGRVGAPLILVVWACFSLLAPVYYDL
                     "
     gene            3408..4166
                     /locus_tag="VGB88_09145"
     CDS             3408..4166
                     /locus_tag="VGB88_09145"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX9647651.1"
                     /translation="MLDTDPRKLRYFYGTAQQPCPYLPGRLECKAVTELSGPDVSQLH
                     DILSRAGFRRSHGIAYRPACHGCHACVPVRVPVAEFRPGRSLRRVAARNADLIVAYRP
                     AIATLEQYELFRVYEHGRHGDGDMALMDFSDYRAMIEDSPVETHVIEFREPGGRLAAV
                     SLTDRLSDGLSGVYKFFALERRRTSPGTYVILWHIEQARALGLSYLYLGYWIADSPKM
                     AYKARFAPLEGLTGECWQRLSLARGVSEAAGAAL"
     gene            4224..5372
                     /locus_tag="VGB88_09150"
     CDS             4224..5372
                     /locus_tag="VGB88_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647652.1"
                     /translation="MNRRDFIAGAATGAVLGAGLTYAAAGPRPRPLDFGTPDAPATAP
                     AVAGAFHEWKMVTTWPRDFPGAGTVAQQLADRITEMSGGRIKVKLFSAGEMVPAFEAF
                     DAVREGTAQCSHDAAYYWVAKNKSIPFFCSVPGGLSQVEHNAWILYGGGQQLWDELYA
                     AFGLRAFMAGNTGTQMAGWFQKEINSLGDLKGLKIRIPGLAAEVLNRLGSTAVNLPAG
                     EIMPALQSGVIDAAEWVGPWVDLTFGFYKVAKYYYGPGFHEGSTANELLINGAAWDSL
                     SSDLKAIVQSACHDANLMLPSEYFARNSESLAVLVDQHGVVVRPLPDDVLEAAFGISA
                     DVAAETASEGELNRRIYDSWSKFRARAIAYQPLSDYGFIRNRALAVGA"
     gene            5450..6550
                     /locus_tag="VGB88_09155"
     CDS             5450..6550
                     /locus_tag="VGB88_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647653.1"
                     /translation="MTTTTPSRRGFLAGLATGAAVAASSLPAPAISQGLVSWKMVTTW
                     PKNFPGLGTGAQRICDRIGALSGGRMEVRLYAAGELVPAFEAFDAVRDGIAEMTHDAS
                     YYWVAKHKSMPFFCTVPGGLTAQEHMGWIYHGGGQKLWDELCAEFGMKAFLAGGSGVQ
                     MGGWFQKEITGLGDFKGLKMRIPGLGAEVVNRLGATAVNLPGGEIMPALQSGVIDATE
                     WVGPWNDLAFGFYKIAKYYYGPGFHEPSSALEVIVSKDRFEALPKDLQAAVENACMAE
                     NGYMLSEFTAGNSEAQRILVDKHGVAIANFPDDVVKAAFDFSVEVVRETATQGDLAKR
                     IFDGWWQVRKDAIARAPFAEQGYMNNRARGTT"
     gene            6739..>7290
                     /locus_tag="VGB88_09160"
     CDS             6739..>7290
                     /locus_tag="VGB88_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647654.1"
                     /translation="MFRHHRVTAPASLIPDRKDWRGQFIIGAAQAAPEHFTTIRLSRI
                     AVHLHEGCSFLRLRCREDTAGLIIGTPIDYAQETVVQTDLDITSYGQLTPAAFEAALE
                     RYIYSFAGTFICVISYRGLNRVYLDANGTKSLVYMPARKLAASTTGLLLNDRDYSDRF
                     DRDLYHRLHVRRDGWFPSGLTGHR"
BASE COUNT         1056 a         2623 c         2496 g         1115 t
ORIGIN      
        1 agctcgcccc gccgccgaac aagagccggc cgtcgccggt cagccggtag tagtcgagca
       61 cggcgcggtc gtcgcagacc gcgactcggt tcgggatcag cgcccgcgcc cgcgcctcgc
      121 ccaacggttc ggtggcacat acatagctcg ctaccggcat cacgcgggca gcaagcgccg
      181 gtaccagacg gccgagatgg gcgttgcccg ccagcaccag cgacttcgcc cgcaccgtcc
      241 cgcgcgccgt ccagacctgc ggctggtcgg cgaatgcgac gcggattgcc ggcgagtctt
      301 cgtaaagccg cgcgccctgc ccttgcgcgg cgtcggcgag gccggcgaga taggcgagcg
      361 ggtcgagatg gcgcgaggtc gcttcgataa ggccgccgtg atagcgctcg ctcgcgacct
      421 cgcgccggac atcgccgcgg tcgagcagcc ggtagccgtc gaagccgtag gcgcgccgcg
      481 ccgtctccgc ccaggcggct tgcgcggcca ggctggcccg cctggtcgcc gcgaccagat
      541 tgcccgcgcc gaggtcgcag gcaatacgca ggcgcgcgat tcgatcgacg atcgcgccga
      601 ggccgtcgat ggtcatgtcc cacagccggc gcgcctcggc gggcgcgaac tggcgcgcga
      661 tcggcgccat gccgggcgag tacccgatca gggcctggcc gccgttgcgg ccggaggcgc
      721 cccagccgag ccggcgcgcc tcgatgagcg cgacgccgac gccgcgccgg gcgagctcga
      781 gcgcgcacga gaccccggcg agcccaccgc cgacgatgca gacctcggca tcgagccggc
      841 cgtcgagcgg cgcccgggcc gcgaccgcgc cgaggcgggc ggcgtagtag ctgtcgagct
      901 gtgccggggc gacgtccatg gcggcaagct agccgctcgg gcgccgacag cgctattggc
      961 atcgcgcacg gccccgatca tgatggccgt atgcccgatt cccgcgaccc gactggcgcc
     1021 gcgacggttg agttcgaccc cgcgcggccg gcgagcttcc cgttctggac ccgcgacgtg
     1081 ctgcgctggg gcgacaccga cgcgctcggc catatcaaca atgtcgagtt cgcgcgcttc
     1141 ttcgagtcgg ggcgcatcgc ctatctgagc gcggccgggg tgcgcggcat cgacgccgac
     1201 gacttcctgc tcgcccatct ggcgatcgac tttcacgccc agatgcacta cccgggcgag
     1261 gtgcggacgg gcacgcgggt gatccggatc gggcgcagct cggtccgctt cgcccaggcg
     1321 ctgttccagg acgacctctg caccgcgtcc ggggtggcgg tcgcggtcat ggccgggcgc
     1381 gacaccgggc aagcgaagcc ggtgcccgag gacatgcgcg cgcggctgct cgatccggga
     1441 gcctgaccgg ccggtcgagc gcccggcgac acaaccgatg gcaggcgaag cagtcctcgc
     1501 cacccagccc tggcagatgt gggcggtgtt cgtgctgatc gcgctcgcgg tcgccgcctt
     1561 ggtcgccgag cgggtggcga tcgagatcac cgccgcgctg gtgctggcgg cgctgctgat
     1621 gctgttccag ctcgcgccgc tggtcgagga aagcggcgcc gaccggctcg attcatcacg
     1681 gctgctgagc ggcttcgcca atccggggct gatcaccgtg gttgcgctgc tggtgatcgg
     1741 ccaggcgttg atccagaccg gcgcgctcga aggcgtcgcc aacctgctct accgcgtcag
     1801 ccgcggccgg ctgatgcgcg ccatggcgct gagcctggtg ccggtcgccg cactgtcgag
     1861 cgtgctcaac aacacgccgg tggtggtgat cttcatcccg atcatgagcg cgctcgccga
     1921 gcgcctgcgc atctcgacca gccgggtgct gatgccgctc agcttcgccg cgatcctggg
     1981 cggcatgacc accttgatcg gctcgtcgac caacctgctt gtcgcgggca ccatccgcga
     2041 cctcggccag cccgcgatcg gctttttcga cttcgccgtg ccggggctgg tgctggccgg
     2101 agtcggcctc gcctacctct tgttcgtggc gccgtggctg atgccggacc gcgcctcgct
     2161 ggccgggcgc ctggtcgggg cggggcgcca gttcatcgcc caggtcgagg tccaggcggg
     2221 atcgccgctg atcggcgagc gcccgatcgc gggcatgttc cgcaagctgc ccgacatcac
     2281 cgtgcgcacc gtccagcgcg gcgagcacgc cttcctgccg ccgttcgacg acgtcacctt
     2341 gcaggcgggc gacgtgatcg tcgccgccgc cacccgcaag gcgctgaccg gcgcgctggc
     2401 caagcacgcc ggcgcgctcc accctagcct cacccatcgc cagatcaccg tcgcgcccga
     2461 ccagcacccg gacgccgacc ggccgtggcg gggcggcgag caggcgctgg ccgaggcgat
     2521 ggtcacgccc ggctcgcgga tgatcggcca gacgctcgag cagttcggct tccgccacca
     2581 gttcaggtgc atcgtggtcg gggtccagcg gcgctcgcgc atgctccgcc agcggctgac
     2641 cgacatccgc ctcgaggcgg gcgacgtgct gctgatccag ggccggccgg aggacgtgcg
     2701 cgcgctcagg gccaatcgcg acgtggtgct gatcgaatgg accgaggaga cgttgccgaa
     2761 atacgtccac gcccggcgcg ccggggcgat cttcgccgcg gtggtggtgc tggctgcgac
     2821 cggcgcggtg ccgatcgtgg tcgccgcggt ggtcggcgcc gccgccatgc tgatcgccgg
     2881 ctgcctcaac gtgcgccagg ccggacgcgc gatcgaccgc gcggtggtga tgaccgtggc
     2941 cgcggcgctg gcgctcgccg ccgccctgca ggagaccggc ggcgcgacct tcctcgcccg
     3001 ccagctgatc gccgcgctcg gcgattcggg ccccggggtc atgctgtcgg cgctgttcct
     3061 gctcatcgcg ctggtcacca acctgctcac caacaatgcc gctgcggtgc tgttcacgcc
     3121 gatcgcggtc agcctggcgc tcgagctcgg cgtcgacccg accgccttca tccacgcggt
     3181 catcttcgcc gccagctgct cgttcgcctc cccgatcggc taccagacca acctgatggt
     3241 gatggccccg ggccattacc gcttcgccga ctacgggcgc gtcggcgcgc cgctgatcct
     3301 ggtggtgtgg gcctgtttct cgctgctcgc tccggtatat tatgacctgt gactcgattc
     3361 gatcaggcgc cggggacccc gccgccggtt gatgacgccg agagccgatg ctcgataccg
     3421 acccgcgcaa gctccgttat ttctacggca ccgcccagca accgtgcccg tacctgccgg
     3481 gccggctcga gtgcaaggcg gtgaccgagc tgtcgggccc tgacgtgagc cagctccacg
     3541 acatcctcag ccgggccggg ttccgccgca gccacggcat cgcctatcgc cccgcctgtc
     3601 acggctgcca tgcctgcgtg ccggtgcggg tgccggtggc cgagttccgg cccggccggt
     3661 cgctgcgccg ggtagcggcg cgcaacgccg acctgatcgt cgcctaccgg ccggcgatcg
     3721 cgaccctgga gcaatacgag ctgttccgcg tctacgagca cggccgccac ggcgacggcg
     3781 acatggcgct gatggacttc agcgactacc gggcgatgat cgaggacagc ccggtcgaga
     3841 cccacgtgat cgagttcagg gagccgggcg ggcggcttgc cgcggtgtcg ctcaccgacc
     3901 ggctcagcga cggcctgtcc ggcgtttaca agttcttcgc cctcgagcgc cggcgcacca
     3961 gccccggcac ctacgtgatc ctgtggcaca tcgagcaggc gcgcgcgctc ggcctgtcgt
     4021 acctgtatct cggctactgg atcgccgaca gcccgaagat ggcctacaag gcgcggttcg
     4081 cgccgctcga gggcctgacc ggcgagtgct ggcagcgcct gtccctggcg cgcggcgtga
     4141 gcgaggccgc gggcgccgcg ctttgaccgc ccggagcgct ctcctatact gaccgcccgc
     4201 gcgggcgaac cagggaggca gcagtgaatc gtcgcgattt catcgccggg gccgccaccg
     4261 gcgcggtgct cggcgcgggc ctgacctatg ccgcggcggg accgcggccg aggccactcg
     4321 atttcgggac gcccgacgcg ccggccacgg cgccggcggt tgcgggcgca ttccacgagt
     4381 ggaagatggt gaccacctgg ccgcgcgact tccccggtgc cggcaccgtc gcccagcagc
     4441 tcgccgaccg catcaccgag atgtccggcg gccggatcaa agtgaagctg ttctccgccg
     4501 gcgagatggt gccggcgttc gaggcgttcg acgccgtgcg cgagggcacc gcccagtgca
     4561 gccacgacgc ggcctactac tgggtggcca agaacaagag catcccgttc ttctgctccg
     4621 tccccggcgg cctcagccag gtcgagcaca acgcctggat cctgtacggc ggcggccagc
     4681 agctgtggga cgagctgtac gccgccttcg gcctgcgcgc gttcatggcc ggcaacaccg
     4741 gcacccagat ggccggctgg ttccagaagg agatcaactc gctcggcgac ctcaagggtc
     4801 tcaagatccg cattcccggc ctcgccgccg aggtgctcaa ccggctcggc tcgaccgcgg
     4861 tcaacctgcc ggcgggcgag atcatgccgg cgctgcagtc gggcgtgatc gacgccgccg
     4921 agtgggtcgg cccgtgggtc gatctcacct tcggcttcta caaggtcgcc aagtactatt
     4981 acgggccggg cttccacgag ggcagcaccg ccaacgagct gctgatcaac ggcgccgcct
     5041 gggattcgtt gtcgagcgac ctcaaggcga tcgtgcagtc tgcctgccac gacgccaacc
     5101 tgatgttgcc gagcgagtat ttcgcgcgca actcggagtc gctggcggtg ctggtcgacc
     5161 agcacggcgt ggtggtacgg ccgctgcccg acgacgtgct cgaggccgcg ttcggcatct
     5221 cggccgacgt cgccgccgag accgcgtccg agggcgagct caaccggcgc atctatgaca
     5281 gctggtcgaa gttccgcgcg cgcgcgatcg cctaccagcc gttgtcggat tacggcttca
     5341 tccgcaaccg cgcgctggcg gtgggcgcgt gacgggcgcc ggcgcggcgg tcaatcgggg
     5401 tcggcgagca tccgacaccc ctgtcaaacc aacaccggaa gggcaagcga tgaccacgac
     5461 gaccccgtcc cgacgcggct ttctcgccgg cctggccacc ggcgcggcgg tcgcggcgtc
     5521 gagcctgccg gcgccggcaa tctcgcaagg cctggtgagc tggaagatgg tcactacctg
     5581 gcccaagaac tttcccggcc tcggcaccgg ggcccagcgc atctgcgacc ggatcggggc
     5641 gctgagcggc gggcgcatgg aggtgcgcct gtacgcggcg ggcgagctgg tgccggcgtt
     5701 cgaggcgttc gacgcggtgc gcgacggcat cgccgagatg acccacgacg cgagctacta
     5761 ctgggtcgcc aagcacaaga gcatgccgtt cttctgcacc gtgccgggcg ggctgaccgc
     5821 ccaggagcac atgggctgga tctaccacgg cggcggccag aagctgtggg acgagctgtg
     5881 tgcggagttc ggcatgaagg cgttcctcgc cggcggctcg ggcgtccaga tgggcggctg
     5941 gttccagaag gagatcaccg gcctcggcga cttcaagggg ctcaagatgc gcatccccgg
     6001 gctcggcgcc gaggtggtca accggctcgg cgcgaccgcg gtcaaccttc cgggcggcga
     6061 gatcatgccg gcgctgcagt caggcgtgat cgacgccacc gagtgggtcg ggccgtggaa
     6121 cgacctcgcc ttcggcttct acaagatcgc caagtactac tacggcccgg gcttccacga
     6181 gcccagctcg gcgctcgagg tgatcgtctc gaaggacagg ttcgaggccc tgccgaagga
     6241 ccttcaggcc gcggtcgaga acgcgtgcat ggccgagaac ggctacatgc tgagcgagtt
     6301 caccgccggc aacagcgagg cccagcgcat cctggtcgac aagcacgggg tggcgatcgc
     6361 caacttcccc gacgacgtgg tcaaggccgc cttcgacttc tcggtcgagg tggtgcgcga
     6421 gaccgcgacc caaggtgatc tcgccaagcg cattttcgac ggctggtggc aggtgcgcaa
     6481 ggacgcgatc gcgcgcgcgc cgtttgccga gcagggctac atgaacaacc gcgcgcgcgg
     6541 caccacctga tccgcgcgca atcgccgctc ggcgatccgg gcttcgcaga gcctgccgga
     6601 gggcgggctc ccgcctccac gccggccgcc cgccacgcct tcccgcagcg tccctggctc
     6661 attggtgcgg cgccccgaca atgctaggct tcccagaaaa tcctgatacc gctcgatcac
     6721 cgtttccgtc gcatccttat gttccgccat caccgcgtca cggcgccagc gagtcttatc
     6781 cctgaccgca aggactggcg cggccagttt atcatcggcg ccgctcaggc cgccccggag
     6841 cacttcacca cgatacgcct ttccagaatc gcggttcacc tacacgaggg ctgtagtttc
     6901 ctgcgactgc gatgccgaga ggacaccgca ggacttatca tcgggacccc gatcgattat
     6961 gcgcaagaaa ccgtcgtcca aaccgacctc gatatcacgt cctatggcca attaactccg
     7021 gccgcgtttg aggccgccct cgaaagatac atctacagct tcgccggcac gttcatctgt
     7081 gtcatatctt accggggcct caatcgagtc tatctcgacg ccaacggcac caagtccctg
     7141 gtctacatgc cggccaggaa attggccgcc tcgaccaccg gactgttgct gaacgaccgc
     7201 gactatagcg accgcttcga tcgcgacctg tatcaccgcc tgcacgtccg tcgagacggc
     7261 tggtttccct cgggtctcac cggccacaga
//
LOCUS       DASWHU010000251        16331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1234103, whole genome shotgun sequence.
ACCESSION   DASWHU010000251 DASWHU010000000
VERSION     DASWHU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16331
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234103"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGB88_09165"
     CDS             <1..181
                     /locus_tag="VGB88_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9647655.1"
                     /translation="MAVAVGSSCAFLTPIGHQSNTLVMGPGGYRFGDYWRMGLPLEAA
                     IVAVGVPMIAFVWPL"
     gene            complement(188..1273)
                     /locus_tag="VGB88_09170"
     CDS             complement(188..1273)
                     /locus_tag="VGB88_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9647656.1"
                     /translation="MTIAAVETGHFRIPLPVTLSDSTHGDITHFELITARVTDADGRE
                     GLGYTYTVGAGGGAIRSLIETDLAPLLQGADETLIEALWQRMWWRLHFVGRGGAAAFA
                     IAACDIALWDLRARRCGLPLWRLLGGNDPRVAAYAGGIDLQFPLERLLDQTRDNLAKG
                     FRAIKMKVGRDRLSEDVARVRAMRDLLGDDFPLMVDANMRWTADQAVAAALALAELHP
                     YWLEEPTIPDDVAGHVRLRSLAPVPIATGENMHTIYEFARMIEAGGVSFPEPDVANCG
                     GITVWLKVARLAEASNLPVTSHGVHDLHVHLLAAVPNASYLEVHGFGLERFIAEPLRL
                     EHGCAIAPERPGHGVAFDWPALDEHRA"
     gene            1551..2504
                     /locus_tag="VGB88_09175"
     CDS             1551..2504
                     /locus_tag="VGB88_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX9647657.1"
                     /translation="MGQESDGALIGLDWGTTSLRAYRIAAGGAVLEARRAHAGILAVE
                     PDAFEAAFAAAVGDWLAALPAAPVIASGMITSRQGWVEVPYLACPAGEAAIAAALHRH
                     RTPGGRELYFVPGLALAAAGGAPDVMRGEETQVCGALDGSGEARLYLLPGTHSKWVLA
                     RDGRIDWFATFMTGELFAVLRDHSILGRPMVREAQDAGWFARGVAHGRVEGAEAGGLL
                     KRLFSARTLHLVGDLPAAGIASYLSGLLIGAEMREALACVRARAPVESLVVLGDDALA
                     SRYLAAAESSGVEAARGPAHAAARGHWRLARAAGLLADAAA"
     gene            2501..3142
                     /locus_tag="VGB88_09180"
     CDS             2501..3142
                     /locus_tag="VGB88_09180"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014716302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX9647658.1"
                     /translation="MTGLAEALAPLPLIAILRGLAPKRARAVGAVLVEAGFRALEVPL
                     NSPDPMRSIAVLAEAFAGRALVGAGTVLAPDQARAVAAAGGRMVVMPHWDAAVVAATK
                     RAGLWSVPGVATPSEAFAALAAGADALKMFPAEALPPAVVRAWRAVLPPSCLLLPVGG
                     IRPESLGPYLAAGASGFGIGSALFAPTLDDAEIAARARAFAAAWRHAAGGGAP"
     gene            complement(3156..4202)
                     /locus_tag="VGB88_09185"
     CDS             complement(3156..4202)
                     /locus_tag="VGB88_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647659.1"
                     /translation="MRPGLDVTPITARFTFDGCCQAASAPARGTEKLMHVVTVTAAHP
                     GIGGTTLTAWLGHCAAMDGAGLVVFLDAGDEPALLAWAGGADLARATAAAWDESCRAD
                     SLRRLGDEGVGLVLVDCDVSRSDAQISDILEATDLVVILVPPQYDNLDDVAELLDIVG
                     SADKPFVFVINRAGEDEDAVGATVISLAQFGTVSPVILPQYDSCPLPDGVRAANGSAD
                     APGIAGDMRRLWEYCKSRLQKQADIDTRTRQRFAMQAIAARYDRPATFVVPEMVYPCH
                     VMDITPQGLVFTSDQDIPPGVRLRINLPYVGQIDCEVAECDSGKVTASFLIGETRRDE
                     LLDQVAHLPAHARD"
     gene            complement(4438..4725)
                     /locus_tag="VGB88_09190"
     CDS             complement(4438..4725)
                     /locus_tag="VGB88_09190"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEX9647660.1"
                     /translation="MVGPIGAHLEASILHDYLYMAWTDFRPKAVGRDWDFADAVLLAG
                     MKVSKVKKSKLIYDVVHSPIGWAVFRKKPYTLKERMVDWLVQLAASHGRDG"
     gene            complement(4726..4992)
                     /locus_tag="VGB88_09195"
     CDS             complement(4726..4992)
                     /locus_tag="VGB88_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647661.1"
                     /translation="MVTWNPGPDPFPAGVIDDITDFQYLDELKFVVHAQIHVDKTPRS
                     LWQLSASFRARMTVHPKDGAPYPLTIRAPRGLYTDLASVPEPLW"
     gene            complement(5103..5834)
                     /locus_tag="VGB88_09200"
     CDS             complement(5103..5834)
                     /locus_tag="VGB88_09200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647662.1"
                     /translation="MARLDGKVALITGASKGLGRGIARAFAREGANLAMVARDPKPLA
                     VAVKEAAGLGAEVISRAGDVTDAGEVERIFAETEKRFARLDLLVNNAGAFDGGPIDEL
                     DIEAWNKVIATNLTAPLLCTKAAMRIMKRQGGGRIINIGSISASRVRPGMAPYNASKH
                     GLWGLTQTTALEGRDHAITCSMVNPGNILVERRQTSGRVQDDEPMMTPDELAELIVTI
                     AALPPHVEVLETTVIPHRQLYVGRG"
     gene            5980..6555
                     /locus_tag="VGB88_09205"
     CDS             5980..6555
                     /locus_tag="VGB88_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9647663.1"
                     /translation="MTGRIGRLRAALRVVALALPLAAAVALAQEPGGAETDWQAGFEA
                     EGAGDWHGALHHYNRAIQSGELSQKRLARVFRSRGNVRHALGEDGEALKDYETAISLD
                     PNYANAYVSRGVIHHLRGDYIRALEDYDEAIRLEPDNALAYANRGGAHEQLEQIEDAI
                     NDFRTAYQLGLDKDWLVEVLTAYGDLPAREP"
     gene            6593..7663
                     /locus_tag="VGB88_09210"
     CDS             6593..7663
                     /locus_tag="VGB88_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX9647664.1"
                     /translation="MRVAAERLEELCREICRRGGSDENEAALVGRHLVEANLSGHDSH
                     GVGVLPTYIGSLVEGSLRPNLHVTVAADLGAILRLDGNGGYGQVIGTEAMELGIARAR
                     ELGVAVVALRRSHHLGRIGAWGEQCAAAGLVSIHYVNATGYRPLVAPFGARCARYTTN
                     PYCTAIPATGANPAIILDMATSTVAFGKIRVAYNRGELAPEDALIDATGRETRDPGVM
                     FADPRGALQPFGRHKGYGLGLVCELLAGALTNGGGMLPEHHEGTVISNNMLTIILDPA
                     EIADAAWFRNEVDAITAHMKGSPTAEGVDAVMVPGDPERKARAERGANGVPLDEETWG
                     QIVEAAAKLGLGAEEIDRIIGA"
     gene            complement(7650..8618)
                     /locus_tag="VGB88_09215"
     CDS             complement(7650..8618)
                     /locus_tag="VGB88_09215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9647665.1"
                     /translation="MTARAPAPAPDLAGQEIAVLVPCRNEAVAVGGVVRAFAEALPAA
                     TIYVYDNGSTDDTVERARAAGAEVHSEARSGKGNVVRRMFADIDADVYLLVDGDDTYD
                     AAAGPELVARLLEGPHDMVSARRMSEHSAAYRAGHRFGNRLLTALVARIFGQSVDDML
                     SGYRAFSRRYVKSFPAASRGFEIETELTVHALQLRMPIAEVPTAYKERPHGSESKLNT
                     VRDGLRILWTIVILFKEIRPLPFFGLWFVLLAGVSLILAWPVFVEYVATGLVPRLPTA
                     VAATGAMLLAFLSLTCGLILDSVARGRTEAKRLAYLALPAPRIKRR"
     gene            complement(8751..9689)
                     /locus_tag="VGB88_09220"
     CDS             complement(8751..9689)
                     /locus_tag="VGB88_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9647666.1"
                     /translation="MNDTGGGVRPRLRAALAAGSAGRPLALMFVSTMGFSIMHALVRH
                     VSGELHPFEIAFFRNVFGLIVIVPWLMRYGLGLLKTPKLELHLLRSGLNVIAMLCFFY
                     ALSVTPLSEVAALSFTAPIFATILAVLVLGEVVRVRRWLAILFGFAGTFVAIRPGFAE
                     VGLGSMLLIIQAVLWAGALVVIKILGRTDSSITIAAYMTLLMIPLSLVPALLFWQTPS
                     LDQLGWLVLIGVIGTLAQLLMTQALKEGEAAVVMPVDFFKLIWAAILGYVFFAEVPGV
                     YTWIGGTMIFAAATYIAYRESVLRQAPIPEAAATRS"
     gene            complement(9686..9880)
                     /locus_tag="VGB88_09225"
     CDS             complement(9686..9880)
                     /locus_tag="VGB88_09225"
                     /inference="COORDINATES: protein motif:HMM:NF022385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEX9647667.1"
                     /translation="MAGELAQPDIDTLEQLFRVLSEALEHVEADQQRLYLAKLSLVLA
                     GHIGDPELVGQATELARRDL"
     gene            complement(9885..11549)
                     /locus_tag="VGB88_09230"
     CDS             complement(9885..11549)
                     /locus_tag="VGB88_09230"
                     /inference="COORDINATES: protein motif:HMM:NF006002.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9647668.1"
                     /translation="MYFTYTHYPFVRPAELDGAAVRHAVAVVGGGPVGLTAALALARF
                     GVRAVVIEPRDCVSHGSRATCISRRSLEVLDRVGPGPAITAKGVWWHSGRTYFRDRQV
                     FALEAPHSNEDRHPPFLNMQQCAFEQFLVDACHANPAVEIRWHSRVAAIAAGKDGVAL
                     TVETPAGSYRLDADWVVAADGAHSTVREALGLRFEGTSYEGRYLISDIELDTDTPAER
                     RVWFDPPSNPGSTIIMHCQPDNIWRFDSQLRPDEDDEQELEDDRLKARIRRHLDFIGE
                     RGRWSLVWSSLYRAHCLTLPAYRAGRVLFAGDAAHLVPIFGVRGLNSGIEDTLNLGWK
                     LAFVVRGLAPEALLDSYSGERHAATVENHFQATKSTWFMTPPGPGFTLMRDAVLSLAT
                     AEPRVRGLINPRQTSPFVYADSELNTPDGAAAGVALGEVLPNLRVDVRRNGKAADGHL
                     LELAGDGFLGLQFAPAGDEAVDALGMEAARAPVPVTLVRVGDGAQRGAAIAVRDGEIA
                     GRLGARAGELMLFRPDDHLCARLVAPTGPALRRAVLRALGRAPATG"
     gene            11766..12095
                     /locus_tag="VGB88_09235"
     CDS             11766..12095
                     /locus_tag="VGB88_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647669.1"
                     /translation="MTVGHSTTLPLAPEQWLHRRRPVAPAHASAPVRMRRAGESPVPR
                     AAGSVTATHTEAPVAAPGFAAQAIAWDLLLDLRPSPQPARKLVAHYARADGLLANRHP
                     PSSGILV"
     gene            complement(12133..13953)
                     /locus_tag="VGB88_09240"
     CDS             complement(12133..13953)
                     /locus_tag="VGB88_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEX9647670.1"
                     /translation="MTASRAACLALGFCLCLALPGPVGAADERARVGGVERVEGAATA
                     SYGEDRRDLAAADPVLFGDLLETGADARLLARLLDESTITLGADASLLVDEFVYDPGA
                     GARVVALRNLAGAFVLAVNDLKGLVEQRVEVATRVGTVSVERGTVWGGELDDGFALLA
                     LDGTVMLVAETGTVRLAEGEGVTITSPAEPMPVALWPRLKVERALQSVAFKDGRVTLR
                     PDWRDQDDPVAALTGGRPSLDLRYRFERVDQDGFAKRAAAHTVRTRLGFETGALFGFR
                     AMLEGQNVFHIGYPRFDDTVNGRSAYPVVADPDNTEFDQYWIASEHVPRSEIKLGRQR
                     IILDNHRFVGNVGFRQTEQTFDAVRVTTGLIPGVTADYAYLALVNRVFGEDSPVGDFE
                     TDSHLIHLSRYQDGIGHLAGYGYLVDINDRPALSSKTFGLRLNGRRQLGEPWWLLYQA
                     EAAWQSGYANNPNDDDFAYYLVEPGAAYASTSLRLGYEVLGGNGRRALQTPLATLHAF
                     QGTADVFPTTPPDGIADLYLGLNHIFVAPLWLTGTRLSVVGHRFAAERGGAHYGDELD
                     AEIAYPLHDRVMLRLEYAVYDAQRFAADTQKIWASVSFSY"
     gene            complement(13958..16057)
                     /locus_tag="VGB88_09245"
     CDS             complement(13958..16057)
                     /locus_tag="VGB88_09245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9647671.1"
                     /translation="MHLTRRIDLWLALTVLGIALIARLVDPGLLWTLRNDVFDVYQEW
                     RPRPDRASPLRIVDIDDRSLARLGPWPWPRARIADLVTRLSEMGTAVIALDLLFPQSD
                     PASPGVANGRGAALEDALRAAGNVVAGFAETGQALESAPPATKAGLASLGDDPIPFLV
                     PIAGTTGNLAIVEAAAQGNGLISAIPDRDGLVRRMPLLVSYRGEAYPALAAEVLRVAQ
                     GATGYIVRARGASRTVGLGSQSGISDIRIGAVAVPTDPEGRLVIHFARPDGTRVIPAW
                     AVLEGRVPEAELRNTIAIVGGSAAELASARPTPVTTAMSLAAIHAQAIDQILAESWLR
                     RPDWAIAAEMLFILVLGVGVIVLSMRRGAIVSALVGGAGIAAAGAFSWYAYAAQGLLF
                     DPVTPGLMVAAVYLVFSVTGHLRVEGQRKLVKQAFSSYMSPNLVEHFIDNPSSLRLGG
                     ERRECSFVLTDLAGFTSLVESAEAAVVAKLLNAYVDGLTRIALAHQGTLDRVVGDAVA
                     VLFSAPVVQPDHALRAIACALEMDAFSRALQAEQQRLGLAVGRTRIGVNTGDVLIGNV
                     GGDAHSDYRALGDAINTAARLEAANKRLGTNVCVSAATAERCPDLERRPIGTLRLAGK
                     RETVDAYEPLANGSLSVAPIAAYREAFAHLDSDRDRALAAFDALAAAYPSDALVRFHR
                     DRLRRGETGQVIDLQGG"
     gene            16149..>16331
                     /locus_tag="VGB88_09250"
     CDS             16149..>16331
                     /locus_tag="VGB88_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9647672.1"
                     /translation="MTAKAKRLQASGGLDIAPLSAALGAEIGGVDLSGPLDDDLVAAL
                     RRALLDHLVVFFRDQRL"
BASE COUNT         2448 a         5679 c         5899 g         2305 t
ORIGIN      
        1 gatggcggtc gcggtcggct cgagctgcgc ctttctcacg cccatcgggc accagtcgaa
       61 cacgctggtg atggggccgg gcggctatcg cttcggcgac tactggcgga tgggcctgcc
      121 gctggaggcc gcgatcgtcg cagtcggagt gccgatgatc gcgttcgtct ggccgttgta
      181 agacctgtca ggcacggtgc tcgtcgagcg cgggccagtc gaaggcgacg ccgtggcccg
      241 gccgctcggg cgcgatcgcg catccgtgct cgagcctgag cggctcggcg atgaagcgct
      301 cgaggccgaa gccgtgcacc tccaggtacg aggcgttggg caccgcggcc agcaggtgaa
      361 cgtgcaggtc gtggactccg tgcgaggtca ccggcaggtt gctggcctcg gccaggcgcg
      421 ccaccttcag ccacaccgtg atgccgccgc agttggcgac gtcgggctcg gggaacgaca
      481 ccccgcccgc ctcgatcatg cgcgcgaact cgtaaatcgt gtgcatgttc tcgccggtcg
      541 cgatcggcac cggcgcgagg ctgcgcaggc gcacgtggcc ggcgacgtcg tcgggaatcg
      601 tcggctcctc cagccagtag ggatgcagct cggccagggc gagcgccgct gccaccgcct
      661 ggtcggcggt ccagcgcatg ttggcgtcga ccatcaaggg aaaatcgtcg ccgagcaggt
      721 cgcgcatcgc gcgcacccgc gccacgtcct cgctcaggcg gtcgcggccg accttcatct
      781 tgatcgcacg gaagcccttg gccaggttgt cgcgggtctg gtcgagcagc cgctcgagcg
      841 ggaactggag gtcgatgccg ccggcatagg cggcgacgcg cgggtcgttg ccgccgagca
      901 ggcgccacag cggcaagccg cagcggcgcg cgcgcaggtc ccacagcgcg atgtcgcagg
      961 cggcgatggc gaacgccgcg gcgccgccgc ggccgacgaa gtgcagccgc caccacatgc
     1021 gctgccacag cgcctcgatc agggtctcgt cggcgccttg cagcagcggc gcgaggtcgg
     1081 tctcgatcag gctgcggatg gcgccgccgc cggcgccgac ggtataggtg tagccgaggc
     1141 cctctcgccc gtcggcgtcg gtgacgcggg cggtgatcag ctcgaagtgg gtgatgtcgc
     1201 cgtgggtcga atcggacagg gtgacgggga gcggaatccg aaagtggccg gtctcgaccg
     1261 cggcgatggt catggcgggc tccgggacgc gcgcatcgcg cggccggcat tgtgccggcc
     1321 ggctcgccgg cgcacaaggc gctgggcggc gtccaggtcg acggccgcag gcggctgccc
     1381 gcagctactg caacagcgcc gcgatctggt cgagatggtc ccaaaccacg tagacagccg
     1441 tgagggccag gaccgtcgcc gtcttgaggt tccacatggc cctgagatta gacggcgccc
     1501 ggaaccggca tagtattggc ggtcacagga cgacgcgccg gggggcggga atggggcaag
     1561 agagtgacgg cgcgctgatc ggactcgact ggggcaccac ctcgctgcgc gcctacagga
     1621 tcgcggccgg cggcgcggtg ctcgaggcgc gcagggcgca cgccggcatc ctggccgtcg
     1681 agccggacgc cttcgaggcg gcgttcgcgg ccgcggtcgg cgactggctc gcggccctgc
     1741 cggcggcgcc ggtgatcgcc tcgggcatga tcaccagccg ccagggctgg gtcgaggtgc
     1801 cctatctcgc ctgcccagcg ggcgaggcgg cgattgccgc cgccttgcac cgccaccgca
     1861 cccctggcgg ccgcgagctt tacttcgtgc ccggcctcgc gctcgccgcc gcgggcggcg
     1921 cgcccgacgt gatgcgcggc gaggagaccc aggtctgcgg cgcgctcgac ggcagcggcg
     1981 aggcgcgcct gtacctgctg cccggcaccc acagcaaatg ggtgcttgcc cgggacggcc
     2041 gcatcgactg gttcgcgacc ttcatgaccg gcgagctgtt cgccgtgctc agggatcatt
     2101 cgatccttgg ccggccgatg gttcgcgagg cacaggatgc gggctggttc gcgcgcgggg
     2161 tggcgcacgg ccgcgtcgag ggcgcggagg ccggcgggtt gctcaagcgc ctgttcagcg
     2221 cgcgcacgct ccatctggtg ggcgacctgc cggcggcggg aatcgcgtcg tatctctccg
     2281 gcctgctaat cggtgccgag atgcgcgagg cgctggcgtg cgtgcgggcg cgggcgccgg
     2341 tcgagtcgct ggtcgtgctc ggtgacgacg cgctggcgag ccgctacctg gcggcggccg
     2401 agtcgagcgg cgtcgaggcc gcgcgcggcc ccgcgcacgc cgctgcgcgc ggccactggc
     2461 ggctcgcgcg cgcggccggg ctgttggcgg atgcggcggc atgaccggcc tcgccgaggc
     2521 gctggcgccg ctgccgctga tcgcgatcct gcgcgggctc gctcctaagc gcgcgcgcgc
     2581 ggtcggggcg gtgctggtcg aggccgggtt ccgggccctc gaggtgccgc tcaactcgcc
     2641 cgatccgatg cgctcgatcg ccgtgctcgc cgaggcgttc gccggccgcg cgctggtcgg
     2701 cgccggcacc gtactcgcgc ccgaccaggc gcgtgcggtg gcggcggccg gcggccgcat
     2761 ggtggtgatg ccgcactggg acgcggcggt ggtcgccgcg accaagcggg caggtctgtg
     2821 gtcggtgccc ggggtggcga ccccgagcga ggcgttcgcc gcgctcgccg ccggcgccga
     2881 tgcgctcaag atgttcccgg ccgaggcgct gccgccggcg gtggtcaggg cgtggcgggc
     2941 ggtgctgccg ccgtcgtgcc tgctgctgcc ggtcggcggc atccgtcccg aaagcctcgg
     3001 cccctatctt gccgccggcg ccagcggctt cggcatcggc tccgccctgt tcgcgcccac
     3061 cctggatgac gccgagatcg cggctcgcgc ccgcgccttc gccgcggcgt ggcggcatgc
     3121 ggcgggtggc ggcgcgccgt aggctgcggt ggaggtcagt cgcgggcgtg cgcgggcaga
     3181 tgggcgacct ggtccagaag ctcgtcgcgc cgggtctcgc cgatcaggaa gcttgcagtc
     3241 accttgcctg agtcgcattc cgcaacctcg cagtcgatct ggcccacata tggcaggttg
     3301 atgcgcagcc gcacgcccgg cgggatgtcc tggtcggagg tgaacacgag gccctgcggc
     3361 gtgatgtcca tgacatggca tggatagacc atttccggca ccacgaatgt cgccggccga
     3421 tcgtaccgcg ccgcaattgc ttgcatcgca aaccgttgcc gcgtgcgcgt gtctatgtcg
     3481 gcttgcttct gcaggcggct cttgcagtac tcccaaagcc gccgcatgtc accggcgatg
     3541 cccggtgcgt cggcgctgcc gttggcggca cgcacgccgt cggggagcgg gcaggagtcg
     3601 tattgcggca gtatgaccgg cgagacggtg ccgaactggg cgagcgagat gactgtcgcg
     3661 ccgaccgcgt cctcatcctc gcccgcgcgg ttgatgacga agacgaacgg cttgtccgcg
     3721 gacccgacga tgtccaggag ctcggcgaca tcgtcgagat tgtcgtactg gggcgggacc
     3781 aatatcacca ccaggtccgt cgcctccagg atgtcggaga tctgcgcatc gctccgcgac
     3841 acgtcgcagt cgaccaggac cagcccgacg ccctcgtcgc cgagccgcct caggctgtcg
     3901 gcccggcagg attcatccca cgcggccgca gtggcgcggg cgaggtcggc gccccctgcc
     3961 caggcgagca gcgccggctc gtcacccgca tccaggaaca ccaccaggcc ggcaccgtcc
     4021 attgccgcgc aatggccgag ccaagcggtc agcgtggtcc caccgatgcc tgggtgggcg
     4081 gcagtaacgg tcaccacatg catgagcttt tccgtgccgc gagcgggtgc agacgcggct
     4141 tggcaacagc cgtcaaaggt gaatcttgca gtaatcggcg tcacatcaag gcccggccgc
     4201 atcacgactt ggttgcagcc ggctgcggcc gggccatggg tacgatgtcc gtcagcgccc
     4261 cggccgcgtg ccaaagtcga taggggccgt cacgactcca gatatggtag ctatgagaat
     4321 cgagagcgac catatcttga tgtccggccc ttgggggccc cgggacgggc acgaccggcg
     4381 ggtgtcgcga ccccgatccg gcttacgtcc gcccggccgc cccgagcgac gccgcggtca
     4441 gccgtcgcgg ccgtggctcg cggcgagctg caccagccag tccaccatcc gctctttcag
     4501 cgtgtagggc ttcttgcgga ataccgccca gccgatcggc gagtgcacca cgtcgtagat
     4561 cagcttggac ttcttgacct tcgagacctt catgccggcc agcagcaccg catcggcgaa
     4621 gtcccagtcg cgcccgaccg ccttgggcct gaaatcggtc caggccatgt acaggtagtc
     4681 gtgaaggatg ctggcctcga ggtgggcacc gatcggaccg acgatctacc acagcggctc
     4741 cgggaccgag gcaagatcgg tgtaaaggcc gcgcggcgcc ctgatcgtca gcggataggg
     4801 cgcgccgtcc ttggggtgga ccgtcatgcg cgcgcgaaag ctcgcggaca gctgccacag
     4861 cgagcgcgga gtcttgtcga cgtggatctg tgcgtgaacg acgaacttca gctcgtcgag
     4921 gtactggaag tcggtgatgt cgtcgataac cccggccggg aacgggtcgg ggccggggtt
     4981 ccatgtaacc atccttattc ccccccaagt cgtgggcgag gcgtgccggc catcggcggc
     5041 cgcgcgcggc gttaccgcat ctcaggacga ccttgagggg ccgatcgttc catccgcctt
     5101 ccctaacccc gcccgacgta gagctgccgg tgcgggatca ccgtcgtctc gagcacctcg
     5161 acatggggcg gcagcgcggc gatggtgacg atcagctcgg ccagctcgtc gggggtcatc
     5221 atcggctcgt cgtcctggac gcggccgctc gtctggcggc gctcgaccag gatgttgccg
     5281 ggattgacca tcgagcaggt gatggcgtgg tcgcggccct cgagcgcggt cgtctgggtc
     5341 aacccccaca ggccgtgctt tgaggcattg tacggcgcca ttcccgggcg cacgcggctg
     5401 gccgagatcg agccgatgtt gatgatccgc ccgccgccct ggcgcttcat gatccgcatc
     5461 gccgctttcg tgcacagcag cggcgcggtg agattggtcg cgatcacctt gttccaggcc
     5521 tcgatgtcga gctcgtcgat cgggccgccg tcgaacgcgc cggcgttgtt gaccagcagg
     5581 tcgaggcgag cgaagcgctt ctcggtctcg gcgaagatgc gctcgacctc gcccgcatcg
     5641 gtgacgtcgc cggcgcggct gatcacctcg gcgccgagcc cggcggcctc cttgaccgcg
     5701 acggcgagcg gcttcgggtc gcgcgccacc atggcgagat tcgcgccctc gcgcgcgaag
     5761 gcgcgggcga tgccgcggcc gaggcccttg ctcgcgcccg tgatcagggc gacctttccg
     5821 tccagtctcg ccatctcgtg cggctcctgt ggtggctgtg atgcgggatg cgcgggcgca
     5881 gtctaggggt cgcaatccgg ccgggcaatg cgttgaacgg ggtcgggtgc catgccattc
     5941 tgcccgcccg caggcgccca ggtggaaatt gcgatggtgg tgacaggcag gattggtagg
     6001 cttcgcgccg ccttgcgcgt cgtcgcgctg gccctgccgc tcgcggcggc cgtggcgctc
     6061 gcccaggaac ccggcggcgc cgagaccgac tggcaggccg gcttcgaggc cgagggcgcc
     6121 ggcgactggc acggcgccct ccaccactac aaccgggcga tccagtccgg cgagctgagc
     6181 cagaagcgcc ttgcgcgcgt gtttcgaagc cgcggcaacg tacgccacgc gctcggcgag
     6241 gacggcgagg ccttgaagga ttacgagacc gcgatcagcc tcgacccgaa ctacgccaac
     6301 gcctatgtca gccgcggcgt aatccatcac ctgcgcggcg attacattcg cgcgctcgag
     6361 gattacgacg aagccatccg gctggagccc gacaacgcgc tcgcctacgc caaccggggc
     6421 ggggcgcacg aacagctcga gcagatcgag gacgcgatca acgacttccg caccgcctac
     6481 cagctcggcc tcgacaagga ctggctggtc gaggtgctga ccgcgtacgg cgacctgccg
     6541 gcgcgcgagc cctgaccggc cgcgcccggc acgccgaggg agaggaaggg ggatgcgcgt
     6601 cgccgccgaa cggctcgagg agctgtgccg cgagatctgc cgccgcggcg gctcggacga
     6661 aaacgaggcg gcgctggtcg ggcggcacct ggtcgaagcc aatctgagcg ggcacgacag
     6721 ccacggcgtc ggcgtgctgc cgacctatat cggctcgctg gttgagggca gcctcaggcc
     6781 caacctgcac gtcaccgtcg ctgccgacct cggcgccatc ctgcgcctcg acggcaacgg
     6841 cggctacggc caggtgatcg gcaccgaggc gatggagctt ggcatcgcgc gcgcgcgcga
     6901 gctcggcgtg gcggtggtcg cgctcaggcg cagccaccac ctgggccgga tcggcgcctg
     6961 gggcgagcag tgcgcggccg cgggcctggt ctcgatccac tacgtcaacg ccaccggcta
     7021 ccggccgctg gtggcgccgt tcggcgcgcg ctgcgcgcgc tacaccacca acccatactg
     7081 caccgcgatc ccggcgaccg gcgccaaccc ggcgatcatc ctcgacatgg cgaccagcac
     7141 cgtcgctttc ggcaagattc gggtcgccta caatcgcggc gagctcgcgc ccgaggacgc
     7201 gctgatcgat gcgaccggcc gtgagacccg cgacccgggc gtcatgttcg ccgacccgcg
     7261 cggtgcgctg cagccgttcg gtcgccacaa gggctacggg ctggggttgg tttgcgagct
     7321 actggctggt gcgctgacca atggcggcgg catgctgccc gagcatcacg agggcaccgt
     7381 gatcagcaac aacatgctga ccatcatcct cgacccggcg gagattgccg acgccgcttg
     7441 gttccgaaac gaggtcgacg cgatcaccgc ccacatgaag ggctcgccca cggccgaggg
     7501 cgtggatgcg gtgatggtgc cgggcgatcc cgagcgcaag gcgcgcgcgg agcggggcgc
     7561 gaacggcgtg ccgctggacg aggagacctg gggtcagatc gtcgaggcgg cggccaagct
     7621 cgggctcggc gccgaggaga tcgaccggat catcggcgct tgatgcgggg cgcgggcagc
     7681 gcgaggtagg cgagccgctt cgcctcggtg cgcccgcgcg cgactgaatc gaggatcaac
     7741 ccgcaggtca gcgacaggaa cgcgagcagc atggccccgg tcgccgcgac cgcggtcggc
     7801 aggcgcggca ccaggccggt cgcgacatat tcgacgaaca ccggccaggc gaggatcagc
     7861 gacacccccg caagcagcac gaaccacagg ccgaagaacg gcagcgggcg gatttccttg
     7921 aacaggatca cgatcgtcca caggatgcgc aggccgtcgc ggacggtgtt gagcttgctc
     7981 tcggagccgt gcgggcgctc cttgtaggcg gtcgggacct cggcgatagg catccgcaat
     8041 tgcagcgcgt gcacggtgag ctcggtctcg atctcgaagc cccgcgaggc ggcggggaag
     8101 gacttgacgt agcggcgcga gaaggcgcgg tagccgctga gcatgtcgtc gacggactgg
     8161 ccgaagatgc gcgcgaccaa cgcggtcagc aggcggttgc cgaagcggtg gccggcccgg
     8221 taggcggccg agtgctcgct catgcgccgt gcggagacca tgtcgtgcgg gccctcgagc
     8281 aggcgcgcga ccagctccgg cccggcggcc gcgtcatagg tgtcgtcgcc gtcgaccaaa
     8341 aggtagacgt cggcgtcgat gtcggcgaac atgcgccgga ccacgttgcc cttgcctgag
     8401 cgcgcctcgc tgtgcacctc ggcgccggcc gcgcgcgcgc gttcgacggt gtcgtcggtc
     8461 gagccgttgt cgtagacgta aatggtcgct gccggcagcg cctcggcgaa ggcgcgcacc
     8521 acgcccccga ccgcgaccgc ctcgttgcgg cacggcacca gcaccgcgat ctcttggccg
     8581 gccaggtccg gcgcaggggc cggcgcgcgc gccgtcatag cctcgctcgc aaccaccggg
     8641 aggtcacgca ccggtttttg tcccaaggca agccaacccc gaccatcgtc ctccggatgt
     8701 gttggaaact agggcacggc tcgcgccaat ccacagcgcc gcgccggggc tcagctccgc
     8761 gtcgccgcgg cttccgggat cggcgcctgg cggagcacgc tctcgcgata ggcgatgtag
     8821 gtggcggcgg cgaagatcat ggtgccgccg atccaggtgt agacgcccgg gacctcggcg
     8881 aagaagacgt agccgaggat cgccgcccag atcagcttga agaagtcgac gggcatcacc
     8941 accgcggcct cgccttcctt gagcgcctgg gtcatcagca gctgggcgag ggtgccgatg
     9001 accccgatca ggaccagcca gccgagctgg tcgaggctcg gagtctgcca gaacaagagc
     9061 gccgggacca gcgacagcgg gatcatcagc agcgtcatgt aggcggcgat ggtgatgctc
     9121 gaatcggtgc ggccgaggat cttgatcacc accagcgccc ccgcccacag caccgcctgg
     9181 atgatcagca gcatcgagcc gagcccgacc tcggcgaagc ccggtcggat cgcgacgaag
     9241 gtgccggcga agccgaacag gatcgcgagc cagcggcgca cgcgcaccac ttcgccgagc
     9301 accagcacgg ccaggatggt ggcgaagatc ggcgcggtga acgacagcgc cgccacctcg
     9361 ctcagcggcg tgaccgagag cgcgtagaag aagcacagca tggcgatcac gttgaggccc
     9421 gagcgcagca ggtgcagctc gagcttgggc gtcttgagca ggcccaagcc gtaacgcatg
     9481 agccacggca cgatcacgat cagcccgaag acgttgcgga agaacgcgat ctcgaacgga
     9541 tgcagctcgc ccgagacgtg gcggaccagc gcgtgcatga tcgagaagcc catggtggag
     9601 acgaacatca gcgccagcgg gcgcccggcc gagcccgccg ccagggccgc gcgcaagcgc
     9661 ggccgcaccc cgccgccggt gtcgttcaaa gatcgcgccg ggcgagctcg gtcgcctgac
     9721 cgaccagctc cggatcgccg atgtggcccg ccagcaccag gctcagcttg gcgagataga
     9781 ggcgctgctg gtcggcctcg acgtgctcga gcgcctcgct gagtacgcga aacagctgct
     9841 cgagcgtgtc gatgtcgggc tgcgcgagct cgccggccat gccctcagcc cgtcgccggc
     9901 gcgcggccca acgcgcgcag cacggcgcgg cgcagcgccg ggccggtcgg cgcaaccagg
     9961 cgcgcgcaca ggtggtcgtc gggcctgaac agcatcagct cgccggcacg cgcgccgagg
    10021 cgtcccgcga tctcgccgtc gcggaccgcg atcgccgcgc cgcgctgcgc tccgtccccg
    10081 acgcggacca gcgtgaccgg caccggtgcg cgtgcggcct ccatgccgag cgcgtcgacc
    10141 gcctcgtcgc ccgccggcgc gaactgcagg ccgaggaagc cgtcgccggc cagctcgagc
    10201 agatggccgt cggcggcctt gccgttgcgc cggacatcga cccgcaggtt cggcaacacc
    10261 tcgccgaggg cgacgccggc ggcggcgccg tccggggtgt tgagctccga gtcggcgtag
    10321 acgaacgggc tcgtctggcg cggattgatc aggccgcgca cccgcggctc cgcggtcgcg
    10381 agcgacagca ccgcatcgcg catcaaggtg aagccggggc ccggcggggt catgaaccag
    10441 gtgctcttgg tcgcctggaa gtggttctcg acggtcgccg cgtggcgctc gccgctgtag
    10501 ctgtcgagca acgcctcggg cgccagcccg cgcaccacga aggcgagctt ccagcccagg
    10561 ttgagcgtgt cctcgatacc cgagttgagc ccgcgcacgc cgaagatcgg caccaggtgc
    10621 gcggcatcgc cggcgaacag cacgcggccc gcgcgatagg cgggcagcgt caggcagtgc
    10681 gcccggtaca ggctcgacca gaccagcgac cagcggccgc gctcaccgat gaagtcgagg
    10741 tgccggcgga tgcgcgcttt cagccggtcg tcctcgagct cctgctcgtc gtcctcgtcg
    10801 ggccggagct gggaatcgaa ccgccagata ttgtccggct ggcagtgcat gatgatggtc
    10861 gagccgggat tggacggcgg gtcgaaccac acccgccgct ccgcaggggt gtcggtgtcg
    10921 agctcgatgt ccgagatcag gtagcggccc tcgtagctgg tgccctcgaa gcgcagcccc
    10981 aaggcctcgc gcaccgtcga gtgggcgccg tcggcggcga ccacccagtc ggcgtcgagc
    11041 cggtagctgc ccgcgggcgt ctccacggtc aaggcgaccc cgtccttacc cgccgcgatc
    11101 gcggcgacgc gcgagtgcca gcgtatctcg accgccgggt tggcgtggca ggcgtcgacc
    11161 aggaactgct cgaaggcgca ttgctgcatg ttcaggaacg gcgggtgccg gtcttcgttg
    11221 gagtgcgggg cctccagcgc gaacacctgg cggtcgcgga aataggtccg gccgctgtgc
    11281 caccacacgc ccttggcggt gatcgcgggc ccggggccga cccggtcgag gacctcgagg
    11341 ctgcgccgcg agatgcaggt cgcgcggctg ccgtggctga cgcagtcgcg cggctcgatc
    11401 accaccgcgc gcaccccgaa acgagccagc gccagcgcgg cggtcagccc gaccggtccg
    11461 ccgccgacca ccgccaccgc gtggcgaacc gcggccccgt cgagctccgc ggggcgcacg
    11521 aaagggtagt gcgtgtacgt gaagtacatt cctatcccgc gtgcaggtca ggaaaggtcg
    11581 gggaccgccg gggagcgaaa aataccaccc cgagcttgcg ccggaaccga aaaaatatta
    11641 ataaaatcct atcaattggc cgcgccggcg ttgacatgcc gatgcggcca tgcgcgaatc
    11701 cttaggggca tcgcaatgaa cgcgatgagt atcggcgccc tgaggagcgg ggacggacgc
    11761 aggcgatgac ggtcgggcac agcacgacgc tgccgctcgc gcccgagcag tggttgcatc
    11821 gacggcggcc ggttgcgccg gcgcacgcct cggcgccggt acgcatgcgc cgggccggcg
    11881 agagtcccgt gccacgtgcg gccggctccg tcactgcgac acataccgaa gcgccggtgg
    11941 ccgcgccggg cttcgccgcc caggcgatcg catgggacct gctgctcgat ttgcgcccct
    12001 cgccacagcc ggcccgcaag ctggtcgcgc actacgcgcg cgccgacggc ctgctggcca
    12061 accgccaccc gccttcatcg ggcattctgg tttgagcgtc cgcgccacgc cgggcgcgca
    12121 cggcccgggc aatcaatagc tgaagctgac gctggcccag atcttctggg tatcggcggc
    12181 gaaacgctgc gcgtcataga cggcgtattc gagccgcagc atcacccgat cgtgcagcgg
    12241 gtacgcgatc tcggcgtcga gctcatcgcc gtaatgcgcg ccgcctcgct cggcggcaaa
    12301 gcggtggccg accacgctca gcctggtgcc ggtgagccac agcggagcca cgaatatgtg
    12361 gttgaggccg agatagaggt ccgcgatgcc gtccggcggc gtggtcggga acacgtcggc
    12421 ggtgccctgg aacgcgtgca aggtcgcgag cggcgtctgc aaggcgcgcc ggccgttgcc
    12481 gccgagcact tcatatccga gccgcagcga ggtgctcgcg taggcggcgc cgggctcgac
    12541 caggtaatag gcgaagtcgt cgtcgttcgg gttgttcgcg taaccgctct gccaggcggc
    12601 ctcggcctgg tagagcagcc accacggctc gccgagctgc ctccgcccgt taaggcgcag
    12661 gccgaaggtc ttggacgaca gggccgggcg gtcgttgatg tcgaccagat agccgtagcc
    12721 cgcgaggtgg ccgatcccgt cctggtatcg ggacaggtgg atgaggtggc tgtcggtctc
    12781 gaagtcgccg accggcgagt cctcgccgaa cacgcgattg accagcgcca ggtaggcgta
    12841 gtcggcggta acgcccggta tcagcccggt ggtcacgcgc acggcgtcga aggtctgctc
    12901 ggtctggcga aacccgacat tgccgacgaa gcgatggttg tcgaggatga tcctctgccg
    12961 gccgagcttg atctccgagc gcggcacgtg ctcgctggcg atccaatact gatcgaactc
    13021 ggtgttgtcg ggatcggcga ccaccggata ggcgctgcgt ccgttgaccg tgtcgtcgaa
    13081 gcggggatat ccgatgtgga acacgttctg gccctcgagc atggcccgaa acccgaacag
    13141 cgccccggtc tcgaagccca gccgggtgcg caccgtgtgc gccgccgccc gcttggcgaa
    13201 gccgtcctgg tcgacacgct cgaaacggta gcgcaggtcg agcgacggcc tgccgccggt
    13261 gagcgccgcg accgggtcgt cctggtcgcg ccagtccggg cgcagcgtca cccgcccgtc
    13321 cttgaacgcc accgactgca gcgcgcgctc gaccttgagc cgcggccata gcgcgaccgg
    13381 catcggctcg gcggggctgg tgatggtgac tccctcgccc tcggcgagcc gcacggtgcc
    13441 ggtttcggcg acgagcatga ccgtgccgtc gagcgcgagc agcgcgaagc cgtcgtcgag
    13501 ctcgcctccc cacacggtcc cgcgctcgac cgagaccgtg cccactcggg tcgctacctc
    13561 gaccctctgc tcgaccaggc ccttgaggtc gttgaccgcg agcacgaagg cgccggccag
    13621 gttgcgcagc gcgaccacgc gcgcaccggc gcccgggtcg tagacgaact catcgaccag
    13681 gagcgaggcg tccgcaccca gggtgatcgt cgactcatcg agcagccgcg cgaggaggcg
    13741 ggcgtcggcg ccggtctcaa gcaggtcgcc gaacagcacc gggtcggcgg cggcgaggtc
    13801 gcgtcggtcc tcgccgtacg acgccgtggc ggcgccctcg acccgttcca cgccgcccac
    13861 ccgcgcccgc tcgtcggcgg cgccgaccgg gccgggcaac gccaggcaaa ggcaaaatcc
    13921 aagcgcaagg caagctgccc gcgaggcggt catcgagtca tcctccctga aggtcgatca
    13981 cctgtccggt ctcgccgcgg cgcaagcggt cgcggtgaaa ccggaccagg gcgtcggacg
    14041 gataggccgc ggccagggcg tcgaaggcgg cgagtgcccg gtcgcggtcc gagtccaagt
    14101 gcgcgaacgc ctcgcggtag gccgcgatcg gcgccacgga caggctgccg ttggcaagcg
    14161 gctcgtaggc gtcgacggtc tcgcgcttgc cggccagccg caaggtgccg atcggccgcc
    14221 gttcgagatc ggggcagcgc tcggcggtcg cggcgctgac gcagacattg gtgccgagcc
    14281 gcttgttggc cgcttccagg cgcgccgccg tgttgatcgc gtcgcccagc gcgcggtagt
    14341 cggaatgggc gtcgcccccg acattgccga tcagcacgtc gccggtgttg accccgatcc
    14401 gggtgcggcc gacggcgagc ccgagccgct gctgctcggc ctgcaacgcg cgcgagaagg
    14461 cgtccatctc gagcgcgcag gcgatggcgc gcagggcatg gtcgggctgc acgaccggcg
    14521 ccgagaacag caccgccacc gcgtcgccga ccacccggtc gagggtgccc tgatgcgcga
    14581 gcgcgatgcg cgtcaagccg tcgacatagg cgttgagtag cttggcgacc accgccgctt
    14641 cggcggactc gaccagcgac gtgaagccgg cgagatcggt cagcacgaag ctgcattcgc
    14701 ggcgctcgcc gccgagcctg aggctgctcg gattgtcgat gaagtgctcg accaggttgg
    14761 gcgacatata gctgctgaac gcttgcttga ccaacttcct ttgaccctcg acgcgcaggt
    14821 ggccggtgac cgagaacacc agatagacgg cggcgaccat cagtcccggc gtcactgggt
    14881 cgaacaagag cccctgcgcc gcatacgcgt accacgagaa ggcgccggcc gcggcgatcc
    14941 cggcgccgcc gaccagcgcc gagacgatcg cgccccggcg catcgacaag acaatcacac
    15001 cgacgccgag gacgagtatg aacaacatct cagccgcgat cgcccagtcg ggacgccgca
    15061 accaggactc ggccaggatc tggtcgatcg cctgggcgtg gatcgcggcc aggctcatcg
    15121 ccgtcgtgac cggggtcggg cgcgcactcg caagctcggc cgcggaaccg ccgacaatgg
    15181 cgatcgtgtt gcgcagctcg gcctcgggca cgcggccttc gagcacggcc caggccggga
    15241 tcacgcgcgt cccgtcgggc cgggcgaaat gaatgaccag ccggccctcg gggtccgtcg
    15301 gcaccgcgac cgcaccgatg cggatgtcgc tgatgcccga ctggctgccg agcccgacgg
    15361 tgcggctcgc tccgcgcgcc ctgacgatat agcccgtcgc tccttgcgcg acgcgcagca
    15421 cctcggcggc aagcgcgggg taggcctcgc cccggtagct caccagcaac ggcatccggc
    15481 gcaccagacc gtcgcggtcg gggatcgcgc tgatcagccc gttgccctgc gccgccgcct
    15541 cgacgatggc gaggttcccg gtcgtgcccg cgatcggcac caggaaggga atggggtcgt
    15601 cgccgaggct tgccagcccg gccttggtcg ccggcggcgc cgactcgagc gcctggccgg
    15661 tctcggcgaa ccctgcgacc acgttgcccg ccgccctgag cgcgtcctca agcgccgcgc
    15721 cccgaccgtt cgcgacgccg ggcgaggccg ggtcggattg cgggaacagc agatcgagcg
    15781 cgatcaccgc cgtgcccatc tcgctcaggc gcgtgaccag gtccgcgatc cgcgcccgcg
    15841 gccacggcca ggggccgagt cgcgccaggc tgcggtcatc gatgtcgacg atgcgcagcg
    15901 ggcttgcccg gtcgggccgc gggcgccact cctgatagac gtcgaagacg tcgttcctga
    15961 gcgtccacag cagcccggga tcgaccagcc gggcgatcaa cgcgatgccg agcacggtga
    16021 gcgcgagcca gaggtcgatg cggcgggtga gatgcatggg tggcgcgaag catagcaaaa
    16081 tgcggcctct tccacagcct gcccggtgcc gattgcggcg ccggccccgg cggtcctatg
    16141 atcggcgcat gacggccaag gcaaaacgcc tccaggcgtc tggtgggctg gacatcgcgc
    16201 cgctgagcgc ggcgctcggc gcggagatcg gcggggtcga cctgtccggg ccgctcgacg
    16261 acgacctcgt cgcggcgctg cgccgggcgc tgctcgacca cctggtcgtc ttcttccgcg
    16321 accagaggct t
//
LOCUS       DASWHU010000252         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3440228, whole genome shotgun sequence.
ACCESSION   DASWHU010000252 DASWHU010000000
VERSION     DASWHU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440228"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGB88_09255"
     CDS             complement(<1..285)
                     /locus_tag="VGB88_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647673.1"
                     /translation="MTVEIRNAAAKDASGIARVHIDTWRDTYAGMLPIQLFRRMGHRQ
                     VSAQWSRTLKYARRSELVLVAEDPDAGIVGFASCASAKGLDLPYRGEVTML"
     gene            488..>1921
                     /locus_tag="VGB88_09260"
     CDS             488..>1921
                     /locus_tag="VGB88_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9647674.1"
                     /translation="MDLATWIERWAAFAPDKAALASDGRTLTYRALAARIAGLAGGLE
                     RELGVRAGDRVAFLGPNCPELVELLFASARIGAIAVPLNWRLALPELAYILDHCGAAL
                     LVCDAAFADAGAALAETRPGLRQVRLGTEFDTLAGAAGGRRGARAGPDSPVLIVYTSG
                     TTGRPKGTVLTQDALFWNAVNSTHMHDLTSADRVLTSAPMFHVGGLNIQTLPALHAGA
                     GVILHRRFDPGAVLAAIARDRPTLTVLVPAQMAALIGHPGWVATDLSSLRMVTTGSTI
                     VPHALIGAFHDRGVPVVQVYGTTETAPIAAYLRAEHARDHVGAAGLAAVHCALRLVDD
                     AGREVGAGERGEVWVQGPNLMREYWDDAAATETALAGGWFRTGDIGHLDAAGFLTIVD
                     RKRDVIISGGENIYPAELERVLADSTAIAEAAVVGRADARWGEVPVAVVVAADRGLTE
                     AAVLGLFAGRIARFKHPKQVLFVDRLPR"
BASE COUNT          251 a          709 c          682 g          279 t
ORIGIN      
        1 gagcatggtg acctcgcccc gataggggag atcgagcccc ttcgcgctcg cgcaactggc
       61 gaagccgacg atgccggcat cggggtcctc ggcgaccagg accagttcgc tccgccgcgc
      121 gtatttgagc gtccgcgacc actgcgcgct gacctgacgg tggcccatcc tgcggaacag
      181 ctggatgggc agcatcccgg cataggtatc ccgccaggta tcgatgtgga cccgggcgat
      241 ccccgaggcg tccttggcgg cggcgttgcg tatctcgacg gtcatgacct tcgccatccg
      301 aaaaagcgag gcgccacggc gcaaatgagc ccgcgcgtca cgcatgacgc gcggccatga
      361 gggatcacgg cgctcgcccg caaagtctac acgtggcggg ttatcgcctc aaatcggatg
      421 ttatcgactg catcggcgcc aggtctcggg tatgagtagg tcgcgcggat gcgttagttt
      481 cggccccatg gacctagcca cctggatcga gcggtgggcg gcgttcgcgc cggacaaggc
      541 ggcgctcgcc tccgacggcc ggacgctcac ctaccgcgcc ctggcggcgc gcatcgccgg
      601 gctcgccggc gggctcgagc gcgagctcgg ggtgcgcgcg ggcgaccgcg tcgccttcct
      661 cggccccaac tgccccgagc tggtcgagct gctgttcgcc tcagcgcgta tcggcgcgat
      721 cgcggtgccg ctcaactggc ggctcgcgct gcccgagctc gcctacatcc tcgaccattg
      781 cggcgccgcg ctgctggttt gcgacgccgc cttcgccgac gccggcgcgg cgctcgccga
      841 gacgcgtccg ggtcttaggc aggtccgcct cggcacggag ttcgacacgc tcgcgggtgc
      901 ggccggcggc cggcgcggcg cgcgcgcggg gcccgactcg ccggtgctga tcgtctacac
      961 ctcgggcacc accgggcggc ccaagggcac ggtgctgacc caggacgcgc tgttctggaa
     1021 cgcggtcaac agcacccaca tgcacgatct caccagcgcc gatcgcgtgc tcaccagcgc
     1081 gccgatgttc catgtcggcg ggctcaacat ccagaccctg ccggcactcc atgccggcgc
     1141 gggcgtgatc ctgcaccgcc gcttcgatcc gggcgcggtg ctggcggcga tcgcgcgcga
     1201 ccgccccacc ctcaccgtgc tggtgccggc gcagatggcg gcgctgatcg gccatcccgg
     1261 ctgggtcgcc accgacctgt cgtcgttgcg catggtcacc accggctcga ccatcgtgcc
     1321 gcacgcgctg atcggtgcct tccacgaccg cggcgtgccg gtggtccagg tctacggcac
     1381 caccgagacc gcgccgatcg ccgcctacct gcgcgccgag cacgcgcgcg accatgtcgg
     1441 cgccgcgggg cttgccgcgg tccactgcgc gctccgcctg gtcgacgacg cggggcgcga
     1501 ggtcggcgcc ggcgagcgcg gcgaggtgtg ggtgcagggc cccaacctga tgcgcgaata
     1561 ctgggacgac gccgcggcaa ccgagacggc gctcgccggc ggctggttcc gcaccggcga
     1621 catcggccat ctcgacgcgg ccggcttcct gaccatcgtc gaccgcaaga gggacgtgat
     1681 catctcgggc ggcgagaaca tctacccggc cgagctcgag cgcgtgctcg ccgactcgac
     1741 cgcgatcgcc gaggccgcgg tggtcgggcg cgcggacgcg cgctggggcg aggtgccggt
     1801 ggcggtggtg gtggcggccg accgcggcct gaccgaggcc gccgtgctcg gcctgttcgc
     1861 cgggcgcatc gcccgcttca agcaccccaa gcaggtgctg ttcgtcgacc gcctgccgcg
     1921 c
//
LOCUS       DASWHU010000253        10792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3103482, whole genome shotgun sequence.
ACCESSION   DASWHU010000253 DASWHU010000000
VERSION     DASWHU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103482"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2646
                     /locus_tag="VGB88_09265"
     CDS             <1..2646
                     /locus_tag="VGB88_09265"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX9647675.1"
                     /translation="DGRAEVRRRFEAGAGGPETAAALAFLADQLISVSYGHADAHVFP
                     QPNPTAADHLALVAVGGYGRGELAPFSDLDLLFVVPYKQTPRGEQIVEYILYLLWDLG
                     LKVGQATRSVTECIARAKADMTIRTALLEARFVYGEQRLFLELKERFAREVAAGSGPQ
                     FLAAKLAERDARHQRMGDSRYVVEPNLKDGKGGLRDLHTLFWVAKYLYRVEAIDELVG
                     RGVLTAAEWRMFDQAHRFLWTVRCHLHYLSGRAEERLTFDVQPQLAVAMQYTDHAGAQ
                     DVERFMKHYYLIAKTVGDLTRIFCAAFEAEHLSKPLLKLRLFGPSTRELDGFRLEHGR
                     LSVLDDAQFERAPVDMIRLFRIAQMRKLDIHPRALMLITRNLKRIDARLRANKEANRL
                     FVDILTNDSEPEVTLKRMNEAGVLGRFVPAFGRVVAQTQHDMYHVFTVDEHTIFAIGI
                     LSGIERGTLGDELPLASEIIHEVAARKVLYLALFLHDIAKGRGGSHSVLGARIAHKLA
                     PRLGYTGEETETVAWLVEHHLLFSSTAFKRDISDPKTVADFVELVRSPQRLRLLLVLT
                     VADIRAVGPSVWNGWKGQLLRSLYYRAEAAMTGGDTLADQRQRVAAAQDAVRAALADW
                     PAADVDAFLERGYPSYWLNFNAEVHARHAAILIEADRAGAPLTVRTRIHEFRAVTEVT
                     VYAHDHPGLFAGITGAIAVCGASIVDARVFTTKDGMALDTIYIQGADGGPFDSPKALA
                     KLASTIEMTLAHDYKPAIALAERARTRLPSRTAVFKVAPRVLFDNRASATHTVIEVNA
                     RDRVGLLYDIARTLSGLNLSIGSAVIATYGETAVDVFYVKDMFGMKLDDERRHEAVRE
                     AILTSIRDGEAAAGLAPQAARTAAE"
     gene            2691..4232
                     /gene="murJ"
                     /locus_tag="VGB88_09270"
     CDS             2691..4232
                     /gene="murJ"
                     /locus_tag="VGB88_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9647676.1"
                     /translation="MALIRSAATVGAYTLASRMLGLLRDILIAKYLGAGMVGDAFFVA
                     LKLPNLFRRLFAEGAFNLAFVPLFAGRIESEGRGPARAFAEDALAVLLWTLVAVTIVA
                     EIAMPWLMYAFAPGFAADAAKFELAVALTRLTFPYLLMISLVALLGGVLNSIERFAAA
                     ASAPILLNLCLIAGLLLFGRRLATPGHALAWAVAVAGIAQFLWLAWSCRRAGLLPALP
                     RPRLTPAVRKLLRVMAPAAVGAGVQQLNLFVDVIIASLLPTGAVSFLYYADRVVQLPL
                     GVIGVALGTALLPALARLERAGDGGAAANAQSRAIEVAMLLTLPAAAALMTVAGPVIT
                     VLFQRGAFGAADSAATAGALAAFAFGLPAYVLIKVLAPGYFARGDTRTPLKVAVACLV
                     VNAALNLILMVPLAHVGIALASALSSWLNVALLGIGLARRGHLSLDARLRARLPRTLA
                     AAAVMAGCLWLAAGLVAPWLAAGLAVKVGALAALIGLGLAVFAVAAQASGAMRLAEVA
                     TLLRK"
     gene            complement(4246..4935)
                     /locus_tag="VGB88_09275"
     CDS             complement(4246..4935)
                     /locus_tag="VGB88_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9647677.1"
                     /translation="MLKFYYSGAPNPTKVALFLEEAGLPYEPIPVDTRRGHQFSPDYL
                     AINPNAKVPAIVDGDVAVFDSNAILLYLAEKTGKFLPEDTPGARGRLLSWLMFVATGV
                     GPYSGQAVHFRQYAPEKIAYAINRYMFEAERHFNIIDKRLGEHRYMLGDAYTIVDMDL
                     WGWARLMPMMLGDEALPKMANLKRLVDEISARPAAQRAVALKERYAFKAEMDDEARRN
                     LFRHLDVRLAS"
     gene            complement(5101..5628)
                     /locus_tag="VGB88_09280"
     CDS             complement(5101..5628)
                     /locus_tag="VGB88_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX9647678.1"
                     /translation="MIRTGCVLLAIGLGLAACGNTAEERGITGAGIGAAGGAVLGAVT
                     GLSVVEGALIGAGAVGLTGALTDKSDIDIGDPIWDRGDDQAAAAPAAPTATAALAPAA
                     VSGPQLVRDIQSGLARMGYDPGPIDGVMGPRTRDAIRQYQGQHGLLVDGQASQALATH
                     IRTTGGTAQAEAATF"
     gene            complement(5767..6708)
                     /locus_tag="VGB88_09285"
     CDS             complement(5767..6708)
                     /locus_tag="VGB88_09285"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9647679.1"
                     /translation="MHPAASIILFTVASGIGYGMLAFQGLHALARAPEPAYGVTAIGV
                     ALALVSAGLASSTFHLGRPERAWRALSQWRSSWLSREGVLALLGYAPALALAAAIALP
                     EALSSLRAPAGLATALLAAATVACTAMIYASLKPIRQWRSPLVVAVYLAYAAAGGVLW
                     MAALARLFGYRPPVETPAVVVTTALCWAVARRYWRHVDRAPARQSAADALGLSPGTRV
                     RPLDAPHTEESYIQREMGFRVARRHARALRRLAEGAGLALPIALAPAAAVLPHWAATA
                     ALVLAAAAFTLGALVQRWLFFAEATHTVTLYYGAPAA"
     gene            complement(6867..7604)
                     /locus_tag="VGB88_09290"
     CDS             complement(6867..7604)
                     /locus_tag="VGB88_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9647680.1"
                     /translation="MTSLPAQHGPTRLGLVIDLDTCVGCHACAVACKEWNTGGVVAPL
                     TDLDPYGPHPDGVWLNRVHSFETETDSGGGRTVNFPRSCLHCEDALCVTVCPTGASYK
                     RAADGIVLVDEDKCIGCKLCSWACPYGAREYDTDSGVMKKCTLCIDRIYNETLAEADR
                     VPACVRTCPASARHFGDLADPGSEVSRLVAARGGRDLMPGLGYRPTNKYLPPRTARAG
                     AGGADALKATGTPESKLLQWVDRLFAR"
     gene            complement(7601..10453)
                     /locus_tag="VGB88_09295"
     CDS             complement(7601..10453)
                     /locus_tag="VGB88_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX9647681.1"
                     /translation="MREPAIQMSAPVGDEVKSTTCYMCACRCGIDVHLKDGVIRFIEG
                     RRDHPVNHGVLCAKGAAGIMTAYSPARLRKPLLRVGERGEGRFREIEWDEALETATHW
                     LAEVRARDPRGLAFFTGRDQSQALTGWWASAYGTPNHAAHGGFCSVNMAAAGLYTIGG
                     SFWEFGEVDWDHTRLLMLFGLAEDHDSNPIKLGLARLKARGAKVVSLNPVRTGYSAIA
                     DQWLGIRPGTDGLLILALIRELLLADKVDFAYLARYTNAPWLVIDDPGAADDGLFARD
                     AAGRPLCWDAAAGAPADALSAGITPALVGRYTLADGRAAVPAFELLARRCLDAAYAPE
                     AVAQRTGIAAAAIRRLAAELAEAAFDHEVVLDVPWTDWAGRRHDKTVGRPVSVLAMRG
                     ISAHSNGFHTCRALHVLQMLLGAIDCPGGFRYRPPFPKPIPPAQKPAGKACAPGAPLA
                     GPPLGYPTGPEDLLVDESGAPLRIDKAFSWEAPLAAHGMMHMVIANAWAGDPYPIDTL
                     FMYMANMAWNSAMNSAGTMAMLADKDPATGGYRIPRIIYSDAFASEMVAYADLVLPDT
                     TYLERTDAISLLDRPISTADGPADSIRRPVIAPDRDVRPFQDVLLDLGARLGLPGMVD
                     EAGRPAFPGGYKDYLVNHQRAPGIGTLAGWRGEAGEAHGRGAPNPAQLDRYVDAGCFW
                     RHQLAPDQRYYKHANRAYLDWAVQMGYVADASPIVLQLYSEPLQRFRLAARGHGPVQP
                     PDRERARIEAAFDPLPLWAPPLEGEAVHGASFPFSAVTQRPAAMYHSWGGHNAWLRQI
                     HARNWLYMHRAAAARLGLADGDWVWVISHHGRIRVRLKTMDGVNPDTVWTWNAIGKRA
                     GAWGLAADAPEARQGFLLNHVISELLPARADGHRTGNSDPVTGQAAWYDLCVRIEPAG
                     AEAGETAPQFAPAVGPALAATRPDALRFGAAFRPRP"
BASE COUNT         1487 a         3876 c         3907 g         1522 t
ORIGIN      
        1 gacggccgcg ccgaggtccg ccgccggttc gaggccggcg cgggcgggcc ggaaaccgcc
       61 gccgcgctcg cctttctcgc cgaccagctg atctcggtca gctacggcca cgccgacgcc
      121 cacgtcttcc cccagcccaa cccgaccgcg gccgaccacc tggcgctggt cgcggtcggc
      181 ggctacgggc gcggcgagct ggcgccgttc tcggacctcg acctcttgtt cgtggtgccc
      241 tacaagcaga ccccgcgcgg cgagcagatc gtcgagtaca tcctgtacct gttgtgggac
      301 ctcggcctga aagtgggcca ggcgacccgc tcggtgaccg agtgcatcgc ccgcgccaag
      361 gccgacatga ccatccgcac cgcgctcctg gaggcgcgct tcgtctacgg cgagcagcgc
      421 ctgttcctcg agctcaagga gcgcttcgcc cgcgaggtcg ccgccggcag cgggccccag
      481 ttcctcgccg ccaagctcgc cgagcgcgac gcccgccacc agcgcatggg cgattcgcgc
      541 tacgtggtcg agcccaacct gaaggacggc aagggggggc tccgcgacct gcacaccctg
      601 ttctgggtcg ccaagtacct gtaccgggtc gaggcgatcg acgagctggt cgggcgcggc
      661 gtgctgaccg ccgccgagtg gcgcatgttc gaccaggcgc accgcttcct gtggaccgtg
      721 cgctgccacc tccattatct ctccgggcgg gcggaggagc ggctcacctt cgacgtccag
      781 ccccagctcg cggtcgccat gcagtacacc gaccacgccg gcgcccagga cgtcgagcgg
      841 ttcatgaagc actactacct gatcgccaag acggtgggcg acctcacccg catcttctgc
      901 gccgcgttcg aggccgagca cctgagcaag ccactcctca agctgcggct gttcgggccg
      961 tcgacgcgcg agctcgacgg cttcaggctc gagcacggcc ggctgtcggt gctcgacgat
     1021 gcgcagttcg agcgcgcgcc ggtggacatg atccgcctgt tccggatcgc ccagatgcgc
     1081 aagctcgaca tccatccgcg cgcgctgatg ctgatcaccc gcaacctcaa gcggatcgat
     1141 gcgcggctgc gcgcgaacaa ggaggcgaac cggctgttcg tcgacatcct gaccaacgac
     1201 agcgagcccg aggtaacgct caagcgcatg aacgaggcgg gcgtcttggg ccgcttcgtg
     1261 cccgccttcg gccgcgtggt ggcgcagacc cagcacgaca tgtaccacgt gttcacggtc
     1321 gacgagcaca ccatctttgc catcggcatc ctgtccggga tcgagcgcgg cacgcttggc
     1381 gacgagctgc cgctggcgag cgagatcatc cacgaggtgg ccgcgcgcaa ggtgctctat
     1441 ctcgccttgt tcctgcacga catcgccaag ggacgcggcg gcagccactc ggtcctcggc
     1501 gcgcgcatcg cgcacaagct cgcgccgcgg ctcggctaca ccggcgagga gaccgagacg
     1561 gtcgcctggc tggtcgagca ccacctcctg ttctcctcga ccgcgttcaa gcgcgacatc
     1621 tcggacccca agacggtcgc cgacttcgtc gagctggtgc gctcgccgca gcggctgcgc
     1681 ctgttgctgg tgctgacggt ggccgacatc cgcgcggtgg gcccaagcgt ctggaacggc
     1741 tggaagggcc agctcttgcg cagcctctac taccgcgccg aggcggcgat gaccggcggc
     1801 gacacccttg ccgaccagcg ccagcgggtc gccgccgccc aggacgcggt gcgcgcggcg
     1861 ctcgccgact ggccggcggc cgacgtcgac gcgttcctcg agcgcggcta cccgtcctac
     1921 tggctcaact tcaacgccga ggtccacgcc cgccacgccg cgatcctcat cgaggccgac
     1981 cgcgccggcg cgccgctcac ggtcaggacc cgcattcacg agttccgcgc ggtgaccgag
     2041 gtcaccgtct acgcccacga ccaccccggg ctgttcgccg gcatcaccgg tgcgatcgcg
     2101 gtgtgcggcg cctcgatcgt cgatgcgcgc gtgttcacca ccaaggacgg catggcgctg
     2161 gatacgatct acatccaggg cgccgacggc ggcccgttcg acagcccgaa ggcgctggcc
     2221 aagctcgcct cgaccatcga gatgaccttg gcgcacgact acaagccggc gatcgcgctc
     2281 gccgagcgcg cgcgcacgcg cctgcccagc cggaccgcgg tgttcaaggt cgcgccgcgg
     2341 gtgctgttcg acaaccgggc gagcgccacc cacaccgtga tcgaggtcaa cgcccgcgac
     2401 cgcgtcggcc tgctctacga catcgcccgc acgctgtcgg ggctcaacct ctcgatcggc
     2461 agcgcggtga tcgcgaccta cggcgagacc gcggtcgacg tgttctacgt caaggacatg
     2521 ttcggcatga agctcgacga cgagcgccgc cacgaggcgg tgcgcgaggc gatcctcacc
     2581 tcgatccgcg acggcgaggc cgcggccggg ctcgcgcccc aggcggcgcg gaccgccgcc
     2641 gagtagcgcc cgccgtcctc ttgccgcgct cctgagcaag gctcgccgcg atggcgctca
     2701 tcagatctgc cgccaccgtc ggcgcctata cgctggcctc gcgcatgctc ggcttgctgc
     2761 gcgacatcct gatcgccaag tacctcggcg ccggcatggt cggcgatgcg ttcttcgtcg
     2821 ccctcaagct gcccaacctg ttccgccggc tgttcgccga gggcgccttc aacctcgcct
     2881 tcgtgccgct gttcgccggg cgcatcgaga gcgaggggcg cgggcccgcg cgcgccttcg
     2941 ccgaggacgc gctcgcggtg ctgctgtgga ccctggtcgc ggtcaccatt gtcgccgaga
     3001 tcgcgatgcc gtggctgatg tacgccttcg ccccgggctt cgccgcggat gcggccaagt
     3061 tcgagctcgc ggtggcgctg acccggctca ccttcccgta tctgttgatg atctcgctgg
     3121 tggcgctgct cggcggcgtg ctcaacagca tcgagcggtt cgccgccgcg gcctcggcgc
     3181 cgatcctgtt gaacctgtgc ctgatcgcgg gcctgctgct gttcggccgg cgcctggcga
     3241 caccggggca cgcgctcgcc tgggcggtgg cggtggcggg catcgcccag ttcctgtggc
     3301 tcgcctggag ctgccggcgc gccgggctgc tgccggcgct gccgcgcccg cgcctgaccc
     3361 cggccgtgag gaagctgctc agggtaatgg cgccggccgc ggtcggcgcc ggggtgcagc
     3421 agctcaacct gttcgtcgac gtgatcatcg cctcgctgct gccgaccggc gcggtgtcgt
     3481 tcctgtacta cgccgaccgc gtggtccagc tgccgctcgg ggtgatcgga gtggcgctcg
     3541 gcacggcgct gttgcccgcc cttgcgcgcc tcgagcgcgc cggcgacggc ggcgcggcgg
     3601 caaacgccca gtcgcgtgcg atcgaagttg ccatgctgct gaccttgccg gcggcggcgg
     3661 cgctgatgac ggttgccggg ccggtgatca cggtgctgtt ccagcgcggc gcgttcggcg
     3721 cggcggacag cgccgccacc gccggcgcgc tcgccgcctt cgccttcggc ctgccggcct
     3781 acgtgctgat caaggtgctg gcgcccggct acttcgcgcg cggcgacacg cgcacgccgc
     3841 tcaaggtggc ggtggcgtgc ctggtggtga atgcggcgct caacctgatc ctgatggtgc
     3901 cgctcgccca tgtcggcatc gcgctggcga gcgcgctgtc gagctggctc aacgtcgccc
     3961 tgctcggcat cggcctcgcg cggcgcggcc atctgagcct ggatgcgcgc ctgcgcgcgc
     4021 gcctgccgcg cacgctcgcc gcggcggcgg tgatggcggg ctgcctgtgg ctcgccgccg
     4081 ggctggtcgc gccctggctc gccgccgggc tcgcggtcaa ggtcggcgca ctcgccgcgc
     4141 tgatcggcct cggcctcgcc gtgttcgcag tcgccgccca ggcgagcggc gcgatgcggc
     4201 tcgccgaggt ggccacgctg ttgcgcaagt gaccgcgggg gacggtcagc tcgccaggcg
     4261 cacgtccaga tggcggaaca ggttgcgccg cgcctcgtcg tccatctcgg ccttgaacgc
     4321 gtagcgctcc ttgagcgcga ccgcgcgctg agccgccggg cgggcgctga tctcgtcgac
     4381 cagccgcttc aagttcgcca tctttggcaa cgcctcgtcg cccagcatca tcggcatcag
     4441 ccgtgcccag ccccacaggt ccatgtcgac gatggtgtag gcatcgccga gcatgtagcg
     4501 gtgctcgccg aggcgtttgt cgatgatgtt gaagtggcgc tccgcctcga acatgtagcg
     4561 gttgatcgcg tacgcgatct tctccggcgc atactgcctg aagtgcaccg cctgcccgga
     4621 ataggggccg acgccggtcg cgacgaacat cagccacgac aacaggcgcc cgcgcgcgcc
     4681 gggcgtgtcc tcgggcagga acttgccggt cttctcggcc aggtagagca ggatcgcgtt
     4741 gctgtcgaac acggccacgt cgccgtcgac gatcgccggg accttggcgt tggggttgat
     4801 cgccaggtag tcgggactga actggtggcc gcggcgcgta tcgaccggga tcggctcgta
     4861 gggcaggccc gcttcctcga ggaacagcgc caccttggtc gggttgggcg caccgctgta
     4921 gtagaacttc aacatgctcc gcctcctgct cgcattgggc cggacccacg gcatcgccgc
     4981 cggccggggc ggagcttaca cggcccggtc aggatttgca gccgcacgcg ggctgccgcc
     5041 accgggcagg tgccggcagc gcgtggcgaa gtgagtcgct accgcgactg gcgggcacgg
     5101 tcagaaggtg gcggcctccg cctgggccgt gccgccggta gtgcggatgt gggtggccaa
     5161 cgcttgtgag gcctggccgt cgaccagcag cccgtgctgc ccctggtatt gccggatcgc
     5221 gtcgcgggtg cgcgggccca tcacgccgtc gatcggcccc ggatcgtagc ccatacgcgc
     5281 caggccgctc tggatgtcgc ggaccagctg cgggcccgac acggccgccg gcgccaacgc
     5341 ggcggtcgcc gtcggcgccg cgggtgccgc cgcggcctgg tcgtcgcccc ggtcccagat
     5401 cgggtcgccg atatcgatat cgctcttgtc ggtgagcgcc ccggtcaggc cgaccgcgcc
     5461 ggcgccgatc aacgccccct cgaccacgct caagccggtc accgcgccga gcaccgcgcc
     5521 gccggcggcg ccgattccgg cgccggtgat gccgcgctct tccgccgtgt tgccgcaggc
     5581 ggcgaggccc agaccgatcg ccaacagaac gcatcccgtg cgaatcatct ccaactcctc
     5641 caagaataga gagtcccggc gctacccggg tattccaagt tgatatggcg gcagcgccgg
     5701 catcgcgcat gctccgggcc gggcgcgcgc gcgtcaagtg tgacggccgt cacaattgag
     5761 cgcccctcag gcggcggggg cgccgtaata gagggtcacg gtgtgggtcg cctcggcgaa
     5821 gaacagccag cgctggacca gcgcgcccaa ggtgaacgcg gcggcggcaa gcaccagcgc
     5881 agccgtggcc gcccagtgcg gcagcaccgc cgcggcgggc gcgagcgcga tcggcagcgc
     5941 cagccccgcg ccctcggcga gacggcgcaa ggcgcgcgcg tgccggcggg caacccggaa
     6001 gcccatctcg cgctggatgt agctctcctc ggtgtgcggc gcatccagcg ggcggacacg
     6061 cgtgccgggg ctgagcccga gcgcgtccgc ggcactctgc cttgccggcg cgcggtcgac
     6121 gtggcgccaa tagcggcgcg ccaccgccca gcacagcgcc gtcgtcacca ccaccgccgg
     6181 cgtctcgacc ggcgggcggt agccgaacag gcgcgccagc gccgccatcc acagcacccc
     6241 accggcggcg gcataagcga ggtagaccgc gaccaccaag gggctgcgcc actggcggat
     6301 cggcttgagg ctggcataga tcatggcggt gcaggcgacc gtcgccgcgg caagcagcgc
     6361 ggtcgcgagc ccggccggcg cgcgcaggct gctcagggcc tcgggcaagg cgatcgcggc
     6421 ggcgagcgcc agcgccggcg catagccgag cagcgccagc acaccttcgc gcgacagcca
     6481 cgacgagcgc cactggctga gcgcccgcca ggcgcgctcg ggccgcccga gatggaacgt
     6541 cgacgacgcc agccccgcgc tcaccagcgc cagcgcaacg ccgatcgcgg tcacgccata
     6601 ggcgggctcc ggcgcgcggg cgagggcgtg caggccctgg aaggcgagca tgccgtagcc
     6661 gatgccggac gcaacggtga acaggatgat cgaggcggcg ggatgcacgg cgctggtttc
     6721 cgatcgcctt gagcgagccc cctcacccag ctccgggtaa ggcgcggcag gtgccgcgcc
     6781 aaccctgcgc aaccctctcc ccccaggcgt ggggggagag ggaagggtga ggggggcggt
     6841 tcgtggctag gcgcgacatg ctgcggtcag cgcgcgaaca ggcggtcgac ccactgcagc
     6901 agcttgctct cgggcgtgcc ggtcgccttg agcgcgtccg cgccgcccgc gcccgcgcga
     6961 gcggtgcgcg gcggcaggta cttgttggtc ggccggtaac cgagccccgg catcaggtcg
     7021 cgcccgccgc gcgccgcgac caggcgcgag acctccgagc cggggtcggc gaggtcgccg
     7081 aaatggcgcg cgctggccgg gcaggtacgc acgcaggccg gcacccggtc ggcctccgcg
     7141 agcgtctcgt tgtagatgcg gtcgatgcac aaggtgcatt tcttcattac gcccgaatcg
     7201 gtgtcgtact cgcgcgcgcc gtacgggcag gcccagctgc acagcttgca gccgatgcac
     7261 ttgtcctcat cgaccagcac gatgccgtcg gccgcgcgct tgtacgaggc gccggtcggg
     7321 cacacggtca cgcacagggc gtcctcgcag tgcaggcacg agcgcgggaa gttgaccgtg
     7381 cgcccgccgc cgctgtcggt ctcggtctcg aagctgtgca cccggttgag ccacaccccg
     7441 tcgggatggg ggccataggg gtcgaggtcg gtgagcgggg cgaccacgcc gccggtgttc
     7501 cactccttgc acgccaccgc gcaggcgtgg cagccgacgc aggtgtcgag gtcgatgacc
     7561 aggccgaggc gcgtcggccc gtgctgcgcc ggcagcgagg tcatggccgc ggccggaagg
     7621 cggcgccgaa gcggagcgcg tcgggccgcg tggcagcgag cgcgggaccc acagcggggg
     7681 cgaactgggg cgcggtctcg cccgcctcgg cgccggcggg ctcgatgcgc acgcacaggt
     7741 cgtaccacgc cgcctggccg gtgaccgggt cggagttgcc ggtgcggtgg ccgtcggccc
     7801 tggccggcag cagctcggag atcacgtggt tgagcaggaa gccctggcgc gcctcgggcg
     7861 cgtccgccgc cagcccccag gcgccggcgc gcttgccgat cgcgttccag gtccacaccg
     7921 tgtcggggtt gaccccgtcc atggtcttca ggcgcacgcg gatgcggccg tggtggctga
     7981 tcacccacac ccagtcgccg tcggcgaggc cgaggcgggc ggcggcggcg cggtgcatgt
     8041 acagccagtt gcgggcgtgg atctggcgca gccacgcatt gtggccgccc cacgagtggt
     8101 acatcgccgc cgggcgctgg gtgaccgcgc tgaacgggaa gctcgcgcca tgcaccgcct
     8161 cgccctcgag cggcggcgcc cacagcggca gcgggtcgaa cgcggcctcg atccgcgccc
     8221 gctcgcggtc gggcggctgc accgggccgt ggccgcgcgc ggccaggcgg aagcgctgca
     8281 gcggctcgct gtagagctgg agcacgatcg ggctcgcgtc ggccacgtaa cccatttgga
     8341 ccgcccagtc cagataggcg cggttggcgt gcttgtagta gcgctggtcg ggcgcgagct
     8401 ggtggcgcca gaaacagccc gcgtccacat agcggtcgag ctgcgcgggg ttgggcgcgc
     8461 cgcggccgtg agcttcgccg gcctcgccgc gccagccggc aagcgtgccg atgccgggcg
     8521 cgcgctgatg gttgaccagg tagtccttgt agccgcccgg gaaggcgggg cggcccgcct
     8581 cgtcgaccat gcccggcagg ccgagccgcg cgccgaggtc caagagcacg tcctggaacg
     8641 ggcgcacgtc gcggtcgggc gcgatcaccg gccggcggat cgagtccgcg ggcccgtcgg
     8701 cggtcgagat cggccggtcg aggagcgaga tcgcgtcggt gcgctcgaga taggtggtgt
     8761 cgggcagcac caggtcggca taggcgacca tctcggaggc gaaggcgtcc gagtagatga
     8821 tgcgcgggat gcggtagccg ccggtcgccg ggtccttgtc ggcgagcatg gccatggtgc
     8881 cggcgctgtt catcgccgag ttccaggcca tgttggccat gtacatgaac agggtgtcga
     8941 tcgggtaggg atcgcccgcc caggcgttgg cgatgaccat gtgcatcatg ccgtgggcgg
     9001 cgagcggcgc ctcccagctg aacgccttgt cgatgcgcag cggcgcgccc gactcatcga
     9061 ccagcaggtc ctcgggcccg gtcggatagc cgagcggcgg gccggcgagc ggcgcgcccg
     9121 gcgcgcaggc cttgccggcc ggcttctgcg ccggggggat cggcttcggg aacggcgggc
     9181 ggtagcggaa cccgcccggg cagtcgatcg cgccgagcag catctgcaat acgtgcagcg
     9241 cgcggcaggt gtggaagccg ttggaatggg ccgagatgcc gcgcatggcg aggaccgaca
     9301 ccggccggcc gacggtcttg tcatggcgcc gcccggccca gtcggtccac ggcacgtcga
     9361 gcaccacctc gtggtcgaag gcggcctcgg cgagctcggc ggcgagccgg cggatcgcgg
     9421 cggcggcgat gccggtgcgc tgggcgaccg cctcgggcgc gtaggccgca tccaggcagc
     9481 ggcgcgccag caactcgaac gcgggcaccg cggcgcgccc gtcggccagc gtgtagcgcc
     9541 cgaccagcgc cggcgtgatg ccggcgctga gcgcatccgc cggcgccccg gcggcggcgt
     9601 cccagcacag cggccggccc gcggcgtcgc gcgcgaacag cccgtcgtcg gccgcgccag
     9661 gatcgtcgat caccagccag ggcgcgttgg tgtagcgggc gagataggcg aaatctacct
     9721 tgtcggcgag cagcagctcg cggatcagcg ccaggatcag aagcccgtcg gtgccgggcc
     9781 ggatgccgag ccactggtcg gcgatcgccg agtagccggt gcgcaccggg ttgagagaga
     9841 ccaccttggc gccgcgcgcc ttcagccggg cgaggccgag cttgatcggg ttcgagtcgt
     9901 ggtcctcggc caggccgaac agcatcagca ggcgggtgtg gtcccagtcg acctcgccga
     9961 actcccagaa cgagccgccg atggtgtaca gccccgccgc cgccatgttg accgagcaga
    10021 agccgccatg ggcggcgtgg ttgggggtgc cgtaggcgct cgcccaccac ccggtcagcg
    10081 cctggctctg gtcgcggccg gtgaagaagg cgagcccgcg cgggtcgcgg gcgcgcacct
    10141 cggcgagcca atgcgtggcg gtctcgagcg cctcgtccca ctcgatctcg cggaaccggc
    10201 cctcgccgcg ctcgcccacc ctgagcaaag gcttcctgag ccgcgccggc gagtaggcgg
    10261 tcatgatccc ggccgcgccc ttggcgcaca gcacgccatg gttgaccggg tggtcgcgcc
    10321 ggccctcgat gaaccggatc acgccgtcct tgaggtggac gtcgatgccg cagcggcagg
    10381 cgcacatgta gcaggtggtc gacttgacct cgtcgccgac cggcgccgac atctgaattg
    10441 cgggctcgcg catggttgcg gcagtcctgg cggaggtggg gcggcggcaa gctagcacag
    10501 gtcaaccgcc ggccggtcaa gatgttggca cgcccccaac aagatagggc acaggacatg
    10561 aaccctgcgg ccgacaggct acgagacaca gcgagcctcc gcgcctgcct tcaagcccga
    10621 aggcgatcgg ggttgccgca ggccaagcgc cgggcctgcc agcgcatggc ttgaagtccc
    10681 tgcacactag tcatataact agtctgtgac aagaccagcg aggatgcagg atgcgacagt
    10741 ggcaggtcca ggaggcgaag gcgcggttca gcgagtttct tgaggcgagc ct
//
LOCUS       DASWHU010000254        33836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2722707, whole genome shotgun sequence.
ACCESSION   DASWHU010000254 DASWHU010000000
VERSION     DASWHU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 33836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33836
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2722707"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..299)
                     /locus_tag="VGB88_09300"
     CDS             complement(48..299)
                     /locus_tag="VGB88_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX9647682.1"
                     /translation="MARPGGIAADQLRSLVERIERLDEERKAIAGDIRDVFAEGKAVG
                     FDVRALRQVIRLRRLEKAERDEQEALLDLYKHALGMDRR"
     gene            460..1071
                     /locus_tag="VGB88_09305"
     CDS             460..1071
                     /locus_tag="VGB88_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX9647683.1"
                     /translation="MTRLRLLACASLLALSASTPARTAELPAGETLAGKLLVAAESMP
                     DPRFQGTVIYICQHSPDGGALGLVLNRRIGVVPGATIAKELNLDAKATDAKVALHWGG
                     PVELGRGFVLHSNDYASESTTVVDPDIAFSVDSQALVDIVEGRGPKRRILVMGYAGWA
                     PGQLESELRRDDWLVEPADPGFVFGDDVDQMWQQAIDRLGVDL"
     gene            complement(1085..4237)
                     /gene="putA"
                     /locus_tag="VGB88_09310"
     CDS             complement(1085..4237)
                     /gene="putA"
                     /locus_tag="VGB88_09310"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX9647684.1"
                     /translation="MILAPAATPPSPSRLAVRAATRADETACVRSLAREAALPVTTAK
                     RIHDRAYRLAGAVRADEDGHGGIDSFLRAYDLTSREGVALMCLAEALLRIPDADTVDR
                     LIRDKIGGVDWHRKFREGESLFVNASTWGLMLTGRVVRLEAGDENHLGAVLARFLARS
                     SEPVIRAAVTRAMRILARQFVIGRTIEEALAQADEAPALDTRFSFDMLGEAACTAADA
                     ERYFEAYLRAVEAVGASAGGRGPVEGPGLSVKLSALHPRYEFAQSRRVMAELVPRLVT
                     LAEPAAAAGLGLTVDAEEADRLEPSLDVVEALASAPKLAGWDGLGVAVQAYQKRARPL
                     IDWLADLARRTRRRLMVRLVKGAYWDTEIKRAQERGLDGYPVFTRKAATDVSYLACAR
                     AILAHADAFFAQFASHNVQTVAAIVELAGRDRPFEFQRLFGMGDALYRHLAEDGKVAV
                     ACRVYAPVGSHEELLPYLVRRLLENGANSSFVNRIHHRELPIESVIADPAARLARLVT
                     APHPAIPLPVDLYPDRRNSRGIDLADPGPLAELGQAMAEAAARTWRAAPIVGGKERDG
                     NARQVRDPARQARVVGEVVEADAAAVDEALGIAAAHAPDWDASGGEARAQCLERAGDL
                     IEEHRAELMALCVREAGKTIAAALAEVREAADYCRYYASQARRLFAAPLALPGPTGER
                     NELTLAGRGTFACISPWNFPLAIFTGQVSAALVAGNSVIAKPAQQTPLIAAAAVRLLH
                     QAGVPAQVLHLLPGAGETVGARLVGDARIDGVAVTGGTDTAQAINRGLAARPGSIVPL
                     IAETGGQNAMIVDSSALAEQVVRDALASAFDSAGQRCSALRVLFLQDDIAGRLIDMLA
                     GAMDELVVGDPARLETDVGPVIDDEAWSRLDAHARRMAVDGRLIRRAPAARDGGEGIF
                     FSPMAFEIDSVDALDGEVFGPILHVLRYRSDRLDAVIDAVNRTGYGLTLGVHSRIEET
                     ARRVIARARVGNIYVNRNMIGAVVGAQPFGGEGLSGTGPKAGGPHYLLRFAVERATAV
                     DTTAAGGNASLLAIDDTADA"
     gene            complement(4234..4650)
                     /locus_tag="VGB88_09315"
     CDS             complement(4234..4650)
                     /locus_tag="VGB88_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX9647685.1"
                     /translation="MDSEPSDSVVGLAGLPVDAAKLAREERLVRDGFWRKLKRALGRI
                     PFADDAVAAFYCAADPQTPTYVRAVLIGALAYFIVPADVIPDVIAGLGFTDDASVLLT
                     ALSAVGGHLKPHHHDQARAFFAGAATRPAEGNPDRP"
     gene            complement(4696..5361)
                     /locus_tag="VGB88_09320"
     CDS             complement(4696..5361)
                     /locus_tag="VGB88_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX9647686.1"
                     /translation="MTILRHPIDFEPITETQRRALGALDTTLLSDAMDGRQTVAALRP
                     VKAGLKLVGQARTVFCPGSVRPAVCAIAIARPGEVLAIDGGGGQAHASLGGIMALDAK
                     AHGLAGGVVDGAVRDIAELRENGLAIFSRGVTPHGAGRDPRGEIDGPILLAGARVLPG
                     DVVVGDDDGVVVIPRTEVDAVIARAEAKRATEAGWIAAIQSGKALAEVHGFTIPEPAA
                     APA"
     gene            5478..6542
                     /locus_tag="VGB88_09325"
     CDS             5478..6542
                     /locus_tag="VGB88_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9647687.1"
                     /translation="MSAVEERQDDTIPVRPAHRFDEAALADYLGGRLDGARGALQVRQ
                     MQGGQSNPTYVIRFARHEYVLRKKPPGKLLPSAHAVDREYRIQKALAGSGLPLAEMRL
                     YCDDDAVIGTPFYVMERMRGRVFHEVTVPGAPPDHRAEIYDQMNKCLAILHGVDWQAR
                     GLADFGKPGSYFARQVSRWTKQWHASKQREIEAMDRLIEWLPEHLPDDDETTVVHGDF
                     RLGNLMIHPTRPEVIAVFDWELSTLGHPLADVGYNLMPYVTPHDAHLGLEGADLDALG
                     IPDMDTYLAAYCRRVGRPVFDPTFHIVFSLFRIAAIVEGVMARGVAGNASSEDAARYG
                     ALTRPYAERGWAMVTRAGLA"
     gene            6544..8178
                     /locus_tag="VGB88_09330"
     CDS             6544..8178
                     /locus_tag="VGB88_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9647688.1"
                     /translation="MPWPDEVRLPMRREAHFGDRVVRCFADRHNDIDQLVRAVVARDP
                     DAEALVLDRTRLSYGALDDQVARVAANLARRCGVGGGDRVALFSGNCIEFALAVLACA
                     RLGAVSVPLGHRLQTPELAYMIGHSGAKVVAVEAQLAERLPARAAMPEMRHLLAFEGD
                     APGCAPFAELLADAPAPPRPDIDEEQVACILYTSGTTGRPKGAMLTHVNIIHTMLHYQ
                     LCMDLSAREHRTLMAVPITHVTGLVAQLLCMIGIGGTTVLMRAFKARAALELISAERI
                     THGVMVPAMYKLMLMDPEFDRFDLSSLRVGGFGGAPMPEATIEELARRAPSVKLRNAY
                     GATETTSPATLLPIGKAATHKHTVGLPLPCADLIVVDEDGRELPRGATGEIWIAGPMV
                     VPGYWRNEDANASEFTGGYWHSGDIGRFTEDGYLQILDRAKDMINRGGYKVFSAEVEN
                     VLVQHPAVVECAVVARPDPVLGERVHAFVLADGEVNADGLRSFCGERLADYKIPEIVT
                     FTDQPLPRNANGKVVKSELRARAVALAAEWGEEATA"
     gene            complement(8267..9583)
                     /locus_tag="VGB88_09335"
     CDS             complement(8267..9583)
                     /locus_tag="VGB88_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9647689.1"
                     /translation="MEQLTDLSTLQSAYRDIEAWLFEDVLVPSTVVQAAFVLAAFVLA
                     RFFAPRLQRWLERLASRRKLELGLTRAGMAAATMSEPIIWLVLQWLSVLIAARAGWPS
                     DLIRIAVSLLTAWVVIRFASQLVRDPTWSKLVALVVWTLAALNILDLLDETMDLLDAL
                     AITLGGLRISVLTVIKGMVSLAVLLWGASLLSRLLERRIGGLPNLTPAVQVLFSKLLK
                     IVLITIAIVAALNTVGIDLTAFAVFSGAVGVGVGFGLQKVVSNLISGVILLLDKSVKP
                     GDVIEVGQTYGWISTLGARYASVVTRDGTEYLIPNEDIITQQVVNWSYSNDAVRLKIP
                     VGISYKSDLRKAIALCLEAAAEAPRVNRTPQSVCLVKGFGDSSVDLELRIWINDPRNG
                     VSNVKSDVLLLIWDKFHANGIEIPFPQRDVHIRSTEHIPVLAQSPA"
     gene            complement(9615..10136)
                     /locus_tag="VGB88_09340"
     CDS             complement(9615..10136)
                     /locus_tag="VGB88_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX9647690.1"
                     /translation="MSEQDGLIAAAILDGRGGGREIGWPEIEAWAPDQGILWVHLDRT
                     GAKTRQWLADSSGLDAVVCQTLLQDDVRPREVADRTLRYVEDLDTARERAAVTMDELS
                     TRLSEQMNRTMYLLTVVAAVLLPPSLITGLFGINVGGMPGIESPWAFPAVVTVLVMIA
                     VAEVLILRRLRWI"
     gene            complement(10166..10954)
                     /locus_tag="VGB88_09345"
     CDS             complement(10166..10954)
                     /locus_tag="VGB88_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9647691.1"
                     /translation="MTSPVLVERRGAIATVTLNRPERLNAIDRVLGEALRETMLALEL
                     DGAVRAVVLRGAGAGFMAGGDLNVFREHMGADLDRVILDITHAFHDAIVSMRRMAKPV
                     IASVHGACAGAGFSTAMACDLVIAADTAFFTLAYSLIGASPDGSSTFFLPRLVGMHKA
                     MELVLLADRFDAAKAAELGLVNKVVPESALEAETATLAERLAAGPTLAYGRAKALLNR
                     SLGSDLADQLDAEAQNIAACSATRDFAEGVAAFLEKRKPSFQGT"
     gene            11111..12415
                     /gene="hisD"
                     /locus_tag="VGB88_09350"
     CDS             11111..12415
                     /gene="hisD"
                     /locus_tag="VGB88_09350"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9647692.1"
                     /translation="MPRFLKTGISGEERAAADDKVRAVVEAALDDIARRGDDAVREMS
                     IKFDRWEREDFRLSEQEIEACLAAVEPRDRADIEFAQAQIRNFARIQRDALKDVEVET
                     LPGVVLGHRNIPVNSVGSYVPGGKYPMVASAHMSVVTAKVAGVARIATCAPPYQGAPA
                     AAIVAAQHLGGADEIYALGGIQAVGAMALGTESIPAVDMLVGPGNAYVAEAKRQLYGR
                     VGIDLFAGPTETLVIADETVDAELCAADLLGQAEHGPTSPVILLTTSDALARATIAEV
                     ERQLAVLATAETAGQAWRDYGQVIVCDDDDEMVRVADEIASEHVQVMTRDPDYFLDHM
                     TNYGALFLGPRTNVAYGDKVIGTNHTLPTRKAARYTGGLWVGKFLKTCTYQRVLTDEA
                     SVRVGEVCSRLCALEGFAGHKEQADIRVRRYAGSNVAGEQTR"
     gene            12412..13182
                     /locus_tag="VGB88_09355"
     CDS             12412..13182
                     /locus_tag="VGB88_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647693.1"
                     /translation="MTEPVPHTPSFALDGRRALVTGAGRGIGRASAIALAGAGAHVVL
                     ASRTASDLEAVAGEIEGAGGSAETAVFDVTDLDAAREAIARIGPVQVLVNNAGTNRPA
                     SLLDVTVEDFDAVMSLNVRAAFFCVQAVARGMIEAGRGGSIINVSSQMGLVALPGRSV
                     YCASKHAVEGMTKAMALDLAPHGIRVNTLSPTFIETPMTRPFFQNKTFLDMVMARLPA
                     GRLGQVSDLMGAVVFLAGDASALMTGTSLTVDGGWTAQ"
     gene            complement(13184..13951)
                     /locus_tag="VGB88_09360"
     CDS             complement(13184..13951)
                     /locus_tag="VGB88_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647694.1"
                     /translation="MDITTCHAIITGGASGLGRATAQRVVADGGKVTILDRDTARGQE
                     AAKELGATARFAELDVTDEAAMTGAVADAVAELGPVTLAVACAGVATPGRILGRAGVL
                     ALDTYQTVIMVNLIGTFNLMKAAAERMQHNQPDADGGRGVIVNTASVAAYEGQVGQAA
                     YASSKGGVVGLTLPAARELAQFGIRVMTIAPGLFLTPMMSGLPEEVQASLAASTPFPK
                     RLGAPAEYADLVACIYRNDMLNGAVIRLDGAVRLAAR"
     gene            complement(14008..14775)
                     /locus_tag="VGB88_09365"
     CDS             complement(14008..14775)
                     /locus_tag="VGB88_09365"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX9647695.1"
                     /translation="MAGPAIGFVAADIPEAAAAYEALTAHYDHVPPERADVVVALGGD
                     GFMIETLHQFMDRGAPIYGMNRGTVGFLMNQYRAEGLVERLTAAEPTVLHPLRMTVTR
                     ADGGRVDGLAINEVSLLRETRFAAHLRVSIDGRVRLELLVCDGVLVATPAGSTAYNLS
                     AHGPILPLGSRLLALTPICAFRPRRWRGALLPESARITVDVLDADQRPVSATADYTEV
                     RGATRVEIHQDPETSLHLLFDPEHNLEERIAREQFTP"
     gene            complement(14825..15814)
                     /gene="moaA"
                     /locus_tag="VGB88_09370"
     CDS             complement(14825..15814)
                     /gene="moaA"
                     /locus_tag="VGB88_09370"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX9647696.1"
                     /translation="MIDPFGRRISYLRVSVTDRCDFRCVYCMAEDMTFLPKSEVLSLE
                     ELDRLCTAFIRLGVRKLRLTGGEPLVRKNVMSLFRSLGRHVRSGDLDELTLTTNASQL
                     ARYADELRACGVRRVNVSLDTLDADKFLAITRWGKLERVMAGLEAADAAGLAVKVNAV
                     ALKGVNEHEFDDLIAWCGERGYDLTLIETMPMGDITGDRTEQYLPLSVVRRRLRERWT
                     LTDIDYTSGGPARYVRVEETGRRLGFITPLTHNFCESCNRVRLTCTGTLFMCLGQEDA
                     ADLRAPLRASESDEPLIAAIQEAISRKPKGHDFVIDRRDRRPAVGRHMSMTGG"
     gene            complement(15944..16132)
                     /locus_tag="VGB88_09375"
     CDS             complement(15944..16132)
                     /locus_tag="VGB88_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HEX9647697.1"
                     /translation="MRDPGLVPGSLVRHPGQPDWGLGQVQTAIGNRVTVNFEHAGKVL
                     INVAVVALEVVEDEGERR"
     gene            complement(16241..16969)
                     /locus_tag="VGB88_09380"
     CDS             complement(16241..16969)
                     /locus_tag="VGB88_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9647698.1"
                     /translation="MRVLLVEDDPATARVTEAVLKSEGMVCEVTDLGEDAIELAKLYD
                     FDIILLDLILPDLDGHEVVRALRDAKVTTPVLILSSLGEIDHKVRGLSAGADDYLTKP
                     FDRAELLARIQAIVRRSKGHADSVIQAGDLAVNLDQRTVEVAGQSVHLTAKEYGILEL
                     LALRKGATLSKEAFLNHLYGGMDEPELKIIDVFVCKLRKKLVAISGHQYIQTVWGRGY
                     MLKDPDDASLRSAIADASSAAAAV"
     gene            17202..18527
                     /gene="fliI"
                     /locus_tag="VGB88_09385"
     CDS             17202..18527
                     /gene="fliI"
                     /locus_tag="VGB88_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX9647699.1"
                     /translation="MTDRFFTDLAHEIDRLPDLRVSGRVAGVRGLLVECDGLQGVLGI
                     GGRCAIIGRSKQPIICESVGFRNDRVLVMPFAPLEGVALGSEVAVIEAQASVHPEMRW
                     LGRVVNARGEPLDGKGPPGIGPRAYPLRARPPPAHSRRRIRGKVDLGVRALNTFITCC
                     RGQRMGIFSASGVGKSVLLSMMARYTSADVTVIGLVGERGREVQEFIQDNLGPDGLAR
                     SVVVVATSDESALMRRQAAYLTLTCAEYFRDQGLDVLCLMDSVTRFAMAQREIGLASG
                     EPPASKGYTPSVFSELPRLLERAGPGVGKGSITGLFAVLVEGDDHDEPIADAVRGILD
                     GHIVMERAIAERGRFPAINVLRSISRTMPNCNSAAENALVTRARALMAAYADMEDMIR
                     LGAYRRGSDALIDEAIDYREPLERFLAQGMDERSSLEDGYAALAGLLAK"
     gene            18565..18975
                     /locus_tag="VGB88_09390"
     CDS             18565..18975
                     /locus_tag="VGB88_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HEX9647700.1"
                     /translation="MRRDALLDRRRVLVELDAGRAVVVRRLAALAAALDDEQRIAAGS
                     LEALYAYRGFAEQVIAERAQLERSMQELDARIAKAREDLAAAFSELKKFEIARDQRLR
                     RERQARAQAEQKALDELALARYRHGRFDPTQSRR"
     gene            complement(19030..19428)
                     /locus_tag="VGB88_09395"
     CDS             complement(19030..19428)
                     /locus_tag="VGB88_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647701.1"
                     /translation="MDDRFETTRLRRSHLAVADNPRPGLDCVITLSETFAGTSLPGPI
                     SISIRYVPDRHFLPQAGLSAYLAAIGTLDWDSLETLGAEIIGDLDSELLPRWVQLRLE
                     AAGATTHRVTFEQRKPDWQDRLMLSRIALD"
     gene            complement(19438..21177)
                     /locus_tag="VGB88_09400"
     CDS             complement(19438..21177)
                     /locus_tag="VGB88_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647702.1"
                     /translation="MSAGLPPLPPPAAGAPAPAADGRTPTAAPTPGAAPSSRDPAPRA
                     DRPVDTLAAGARAGPAPVTAPLSPPEALATAAPGTRLDGAIEVDSSGTTTLFRAAWGD
                     AAVLAATPTGPAAHAVLEVVAAEDALLAVPVGAGGTALIVLAPHAVPGGETAGTLPAA
                     FAELPPGAVVIGTVVPLPAAGTALLVGDAGVVRLEAPAPAALPHSGTRLVLEIVARAR
                     HIIAQQVSPARGAPARLALAPAQGPALARARAQAIAATGARGVLIPGRTVTGTVVEVA
                     TPRRRGPVTTPMVLSAAPKGATVALRLVGFGPSTPETAGAQEVSATVLGRDADGRLLV
                     DMAERTVALPSDAPLPPGTALTFEMLPAASTAPIADPAARSPVIETLARMAAAGAGER
                     AAVMAQIPGGAPSAVLGLIAYLLGLRAGDARLWLGEARARTLERAAGGQLVERLGEDF
                     RHAWRGGDAAGAPDWRQIPLPWQGDGQIHEARLWFGRERGEADAAEDGAEAGTRLVVD
                     VDLSRLGRLRIDGLYRKRRFDVVLRSLRPLDQAARRNLRMIFDDVLAVSGLAGAIEFQ
                     IVSALGRPDSVAT"
     gene            complement(21179..21970)
                     /locus_tag="VGB88_09405"
     CDS             complement(21179..21970)
                     /locus_tag="VGB88_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9647703.1"
                     /translation="MSTPSNVARHPAAAHLVENVIAVASGKGGVGKTWFSITLAHALA
                     ARGRRLLLFDGDLGLANIDVQLGLMPKYDISAVIAGKVTFADAVQWFEDGGFDILPGR
                     SGSGSLAALPPDRLKSLRDGLIALAARYDKVVLDLAAGLDENLRLLAGMSGTCLVLVT
                     DEPTALTDAYALIKVLTAAGHGRRVKVVVNQAASQAEGERTHATLVKACETFLKIAPP
                     LAGVVRRDDRVRDAIRRQVSVFSRSPTSDSVADVARIAATLADNT"
     gene            complement(21967..22944)
                     /locus_tag="VGB88_09410"
     CDS             complement(21967..22944)
                     /locus_tag="VGB88_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647704.1"
                     /translation="MRLRVYTARTIADAMRQIRNELGDDAVIVSTETDTDGEVRITAA
                     LENLDDADVLARLIGDEPASGARAEALGALAYHGVPDDLAGGLVADAGEDDVASLLRA
                     ALAHAFLFEPVKLDPRARPLMMVGTPGAGKTVTTAKLAARATIDGCAVKVISTDTFRA
                     GARAQLAAFTDILNAELVDADGPDELRAAIAAGASDAVVLIDTAGVNPFDPADMAALA
                     RLVEAGGGEPVLVMAAGGDSLEAAEIAGSFAALGAHRLVATRLDVARRLGALLVVAEA
                     GPFAFAEASASAQVALGLTPITPYALTRLLLGEPIASATNSQPEKAAAR"
     gene            complement(22955..25099)
                     /gene="flhA"
                     /locus_tag="VGB88_09415"
     CDS             complement(22955..25099)
                     /gene="flhA"
                     /locus_tag="VGB88_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX9647705.1"
                     /translation="MADSGPQIRGPTAAEPPAAPDLGGVIRRLAATLREGDVALSLCV
                     ITILIVLIMPMPRWLLDFSLAISITFSVLVLMVSLFIARPLEFSSFPTILLIATILRL
                     ALNLASTRLILADGHTGTAAAGKVIQAFGDFVMSGNFVIGLIVFAILVIVNFVVITKG
                     STRIAEVSARFSLDSMPGKQMAIDADLSSGLIDESEARRRRRQLEDESTFFGAMDGAA
                     KFVRGDAIAGLLITFINIIGGIVIGVAQHGLSFADAANTYTVLTVGDGLVSQMPALVV
                     STAAGLLVSKAGVVGSADKAVFGQLASYPKALGLVSALMVAMSLLPGIPMPPFLVLAG
                     LTGFQAWRLSQQARQRSAEVDAQARAQAEASMARPAEEPIATALHIDVLKLELGYGLL
                     PLINSDKGPRLTDQIKALRRQLATDMGFIMPAVRIQDNMQLPANQYLIRVKDIESGVG
                     DLMPHQLLVMDPRGEAIALPGQDTREPTFGLPARWVGESQREEALFRGYTVVDPATVI
                     TTHLTEVVKDHMAELLSYSETQKLLDELGAEHQKLVADLIPGQISLGGVQRVLQCLLA
                     ERVSIRDLPTILEGISEALGYTQNVTLITEHVRARLARQLSHDNSSRAGYVPLVTLAP
                     QWEQAFAESLVGQNDDKRLHMAPSKLQEFITSVRQEFDKHAGEGEMPVLLTSPAIRPY
                     VRSIIERFRPSTVVMSQNEIHPKAKIHTLGQV"
     gene            complement(25121..26485)
                     /locus_tag="VGB88_09420"
     CDS             complement(25121..26485)
                     /locus_tag="VGB88_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9647706.1"
                     /translation="MRLLIIGTLAGQLGEACKIAVARGARVVHADDIAIALHNLRSGA
                     GGGADLVMIDVALDVGQLTRSLAAEHINVPVIACGMGNDSRAAVAAIKAGAKEYLPLP
                     PDPDMIAAVLEAVTEENHRIVFEDPAMREVLDLAERIARSDASILITGDSGTGKEILA
                     RHIHARSRRSAKPFVSVNCAAIPEALLESELFGHEKGAFTGAIARRIGKFEAADGGTL
                     LLDEIGEMDLKLQAKLLRVLQEREIDRVGGTRPVKIDIRLLATTNRDLQQQVAARNFR
                     EDLYFRINVVTVHIPPLRERTRDILLLAEHFIGKYAEANGLAPRPLSKAAAERLLRHA
                     WPGNVRELENNMHRAVLLATGPEIGPEAMMLGGGAGPAAIDGEAGRVAGTGGASRLVG
                     RTVAAVERDLIIDTLDHCLGNRTHAATILGISIRTLRNKLKAYDAEGVAVPPPAAEAR
                     AAGL"
     gene            complement(26520..27287)
                     /locus_tag="VGB88_09425"
     CDS             complement(26520..27287)
                     /locus_tag="VGB88_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9647707.1"
                     /translation="MDIATLLGLVIGFGLVVAAIATGGSISAFIDIPSVLIVIGGTFA
                     VTTVSFSLGEIMKGQGLILKAVFNKAIDPGAAALQVIELADLARKNGVLGMQEKLRDL
                     DNVPFLQKGLSLVVDGTPGDDVERMMKQEIKGMKSRHERGVGILRQAATVAPAMGLIG
                     TLIGLVQMLGNLDDPNSIGPSMAVALLTTLYGAVLANMVFSPLAAKLERNSNDEASVM
                     NVYTLGASSIGRQENPRRLETLLNTLLPPPKRVQYFD"
     gene            complement(27300..27638)
                     /gene="fliN"
                     /locus_tag="VGB88_09430"
     CDS             complement(27300..27638)
                     /gene="fliN"
                     /locus_tag="VGB88_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX9647708.1"
                     /translation="MADNEDLELEEIGSGGAPAPAAAGSDDGPRTAADLESVFDIPVQ
                     VSAVLGKATMQVSQLMRLGRGAVVELDRKVGEAIDIYVNNRLVARGEVVVVEDRLGVT
                     MTEIIKTDRF"
     gene            complement(27631..28317)
                     /locus_tag="VGB88_09435"
     CDS             complement(27631..28317)
                     /locus_tag="VGB88_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX9647709.1"
                     /translation="MTQINKFLFETDFGAEGRTPRRRAGESREGERAAYSDDDLAGAR
                     QSGYESGIAAGRAQATAAIEARIESLLETVAKRLQAVAQSGAEQRLLTRHDTARLAQA
                     IAAKIIGRREHEGQLEVVERLVDECLAEIYGVEQVTVAVDPTLADTLVARLKPPASGI
                     AVTVVGDSSLTGAECRIDWPGGNARRLESELWARIEAILERYTAPTDEPAPAEPQAAG
                     EPSPSGDDHG"
     gene            complement(28334..29350)
                     /gene="fliG"
                     /locus_tag="VGB88_09440"
     CDS             complement(28334..29350)
                     /gene="fliG"
                     /locus_tag="VGB88_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX9647710.1"
                     /translation="MAQTKTELRELRGPQKAAIIMFALDEERAAKLFGMMDLEEIKEL
                     SSIMSQLGNIAAPVVENLFLDFVRQISGTGNLMGSFESTERLLRATLDTDRVEEIMEE
                     LRGPAGRTMWDKLGNVNEQVLANYLKNEYPQTVAVVLSKVKPAHASRVLGLLPDDFGV
                     EVVMRMLRMELVQKDVLEDVERTLRSEFMSNLAASSRRDSHELMAEIFNNFDRASESR
                     FLQALEDRDHDAAERIKALMFTFEDLKKLEGNSIQTLLREIDKDRLGIALKGASEDLR
                     NLFFTNMSERAAKILREDMEALGPVRLSDVEEAQQEIIITAKDMAARGDIVIAGSGED
                     ELVY"
     gene            complement(29387..31078)
                     /gene="fliF"
                     /locus_tag="VGB88_09445"
     CDS             complement(29387..31078)
                     /gene="fliF"
                     /locus_tag="VGB88_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX9647711.1"
                     /translation="MNGLVQTLRSLGAVRLILLAGVGAGLVAFFMFVFTRLTTPDFTL
                     LYGELPVRDSGAIANQLEAMAVPYRIGGDGSRILVPSDQVLRLRLRLAEQGLPSGGSI
                     GYELFDEADSLGATRFVQNVNLVRALEGELARTIGLLSRVGAARVHLVLPQRELFQRE
                     HEQPSASVFLRLQGASGLSQTQIAAIQHLVAAAVRGLMPERVAIVDDRGNLLASGAEE
                     RNEIGFAAARADEFRHAYEAQVKDSLEQLLERSLGPGKVRAEVAAEINFDRTTTNAEV
                     YDPDGQVVRSNQSVEEETTDSEGSGPGSVSVANNLPGGEAGAGPGSQASTARTEETVN
                     FEISRTVSNHIEEGGRVERLSVAVLVDGSTRTDPQSGEQSYVPRTEDELAQIAALARS
                     AIGFDADRGDTIEVINMRFAEPEPVEMPSEPLLGLDKGDYFRIAQILVLALVAVLVIL
                     LVLRPLISRLLTLQAHAGEPAATAPGAIPGPRGQPMLTGPEGAAQPGAGGQARAELPA
                     ARGSGAGADGEGMIDINKVEGQVKASAVKRMGEIVDKHPDEALSILRGWMAESST"
     gene            complement(31270..32604)
                     /locus_tag="VGB88_09450"
     CDS             complement(31270..32604)
                     /locus_tag="VGB88_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX9647712.1"
                     /translation="MSLYGALFAGVSGLNAQTQSMAMISDNIANLNTIGYKRVTARFA
                     TLVTNSGSLATHSPAGVRSTPFYTINQQGQISTTNSDTDVAISGNGFMVVNTETDGSG
                     AARYTRAGSFTPDSLGNLRNSSGYYLQGWALDANGNLPAASADLSSLTTVNIGQFSGT
                     ASATSTVGIGTNLDAGQTIFGGAYAVNDMAAGTVTPHFFRNFVVFDSLGASHELTVAY
                     LKTGINTWAVEVYAAVPAEVTAGATALASGNIVFNGDATLDADNAGTTIGTVDPITSA
                     LETPTSITWTNGASASTIDIDWGTNDLADGVSQFFSNYNVSFLNQNGASVGLLNGVSI
                     DEDGFVVASFTNGQTRSAYKLPISTFADPTSLRTEEGNAYSETNRSGNFNLREAGLGG
                     AGNFVPSALESSNVDIAREFTELIITQRAYSANTRVITTADEMLDELIRLRR"
     gene            complement(32652..33341)
                     /locus_tag="VGB88_09455"
     CDS             complement(32652..33341)
                     /locus_tag="VGB88_09455"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEX9647713.1"
                     /translation="MEVSAVTTKQAQQASVIAGRDLAENFDNFLMLLITQLRNQDPLS
                     PMDTNEFTSQIVRFAGVEQAIATNANLEQLIALQRANQAAGLIGYLGQRIEANGNTTA
                     LADGRAEWNYALPGAANQVTLLVTNEAGRAIYSTTGATEAGAHQFVWSGLDDAGNPMP
                     EGQYTLAVTATDPEGNSMTATMTTVGTVTGIETAGDGPVLFVGKSGVPLGEVLAILGP
                     LSDQQGQQEQT"
     gene            complement(33363..>33836)
                     /locus_tag="VGB88_09460"
     CDS             complement(33363..>33836)
                     /locus_tag="VGB88_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX9647714.1"
                     /translation="LAASAAAAVARAAPPPMPAIDQIVLHIHQAIEDELSHVRIQLHP
                     KELGRVEVRLELGDGGRLSAHVLVQRAETLELMMRDARGLERALQDAGFDLDSDSLAF
                     DLLDRDFGDGHDDRGAGDIAAGPPDPLRAMAEAEIGAGPGALLIGPGAAGIDIRV"
BASE COUNT         4920 a        11996 c        11775 g         5145 t
ORIGIN      
        1 cgacaggcgc aagcgacggc gtgtcaggac gggctcccgc ggaggggcta gcggcgatcc
       61 atgccgaggg cgtgcttgta aaggtcgagc aaggcctcct gctcgtcacg ctcggccttt
      121 tcgaggcgcc tgaggcggat cacctgccgc agcgcccgga cgtcgaagcc cacggccttg
      181 ccttcggcga agacatcgcg gatgtcgccc gcgattgcct tgcgctcctc gtccaggcgc
      241 tcgatgcgct cgaccagcga gcgcagttgg tcggccgcga tcccaccggg cctcgccatg
      301 gggcatccct ccgttgtgac ttgaatccgt gcggcggcgc gcgatcatag gggctcggtc
      361 gcgtcggtgc gaccgttttt gcccatgccg cgggctcggc gccgcgcagg gcggtgcagg
      421 ccgcgcgaat cagcttgacc ggcggccggg gagggatgga tgactcggct tcgcttgctc
      481 gcttgcgcct cgttgctcgc cctgtcggca agcacgccgg cgcgaacggc cgaacttccg
      541 gccggagaaa ccctggccgg caagctcctg gtcgccgccg agtcgatgcc cgatccgcga
      601 tttcagggaa ccgtcatcta catctgccag cacagccccg acggcggcgc gctcggcctg
      661 gtactgaacc ggcgcatcgg cgtcgtcccc ggcgccacca tcgccaagga gctgaacctc
      721 gatgccaagg cgaccgacgc caaggtggcg ctgcactggg gcggcccggt cgagctcggt
      781 aggggcttcg tgctgcacag caacgattac gcgtccgaat cgaccacagt cgtggatccg
      841 gacatcgcct tcagcgtcga cagccaagcg ctggtcgaca tcgtcgaagg ccgcgggccc
      901 aagcggcgca tcctggtgat gggctatgcc ggctgggcgc cggggcagct cgaatccgag
      961 ctccgccgcg acgactggct ggtggagccg gccgacccgg ggttcgtgtt cggcgacgac
     1021 gtcgaccaga tgtggcagca agccatcgac cggctcggcg tcgatctgta atccggcgtc
     1081 ggcatcaggc gtccgcagtg tcgtcgatcg caagcaggct ggcgttgccg ccggcggcgg
     1141 tggtgtcaac tgcggtcgcg cgctcgacgg cgaagcgcaa gaggtaatgg ggtccgccgg
     1201 ccttgggccc ggtcccggac aatccctcgc cgccgaacgg ctgcgcgccg acgacagcgc
     1261 cgatcatgtt gcggttgacg tagatgttgc cgacgcgggc gcgcgcgatc acgcggcgcg
     1321 cggtctcctc gatccggctg tggacgccga gggtgaggcc gtagccggtg cggttgacgg
     1381 cgtcgatcac cgcgtccagc cggtcggagc ggtagcggag cacgtgcaat atcggcccga
     1441 aaacctcgcc atcgagcgca tcgaccgagt cgatctcgaa cgccatgggg ctgaagaaga
     1501 tgccctcgcc gccgtcgcgg gcggcgggcg cccggcggat cagccgcccg tccacggcca
     1561 tgcggcgcgc gtgcgcgtcg agccgggacc aggcctcgtc gtcgatcacc ggcccgacgt
     1621 cggtctcgag ccgggccggg tcgccgacca ccagctcgtc catcgcgccg gccagcatgt
     1681 cgatcagtcg accggcgatg tcgtcctgca ggaacaagac gcgcagcgcc gagcagcgtt
     1741 ggccggcgct gtcgaaggcc gaggcgagcg cgtcgcgcac cacttgctcg gcgagtgcgc
     1801 tcgagtcgac gatcatcgcg ttctggccgc cggtctcggc gatcagcggc acgatcgagc
     1861 ccgggcgcgc cgcgaggccg cggttgatcg cctgcgcggt gtcggtgccg ccggtgacgg
     1921 cgacgccgtc gatccgcgca tcgccgacca ggcgcgcgcc gaccgtctcg cccgcgcccg
     1981 gcaacaagtg gagcacctgc gccggcacgc cggcctgatg cagcagccgc accgcggcgg
     2041 cagcgatcaa cggcgtctgc tgggcgggct tggcgatgac cgagttgccg gcgaccagcg
     2101 ccgcgctcac ctggccggtg aagatggcga gcgggaagtt ccaggggctg atgcaggcga
     2161 aggtgccacg cccggccaag gtcagctcgt tgcgttcgcc ggtcggcccg ggcagggcga
     2221 gcggcgccgc gaacagccgg cgcgcctgcg acgcgtagta gcggcaatag tccgcggcct
     2281 cgcgcacctc ggcgagcgcg gcggcaatcg tcttgccggc ttcgcgcacg catagcgcca
     2341 tcagctcggc gcggtgctcc tcgatcaggt cgccggcacg ctcgaggcac tgcgctcgcg
     2401 cctcgccgcc gctcgcatcc cagtcggggg cgtgtgccgc ggcgatgccg agcgcctcgt
     2461 cgaccgccgc ggcatcggcc tcgaccacct cgccgaccac gcgcgcctgc cgcgccgggt
     2521 cgcgaacctg gcgcgcgttc ccgtcccgct ccttgccgcc gacgatcggc gcggcgcgcc
     2581 aggtccgcgc cgccgcctcg gccatggctt ggccgagctc ggccagcggt cccgggtcgg
     2641 caaggtcgat gccgcgcgaa ttgcgccggt cggggtaaag gtcaaccggc aaggggatcg
     2701 ccgggtgggg cgccgtcacc agccgcgcca gcctggcggc cgggtcggcg atcaccgact
     2761 cgatcggcag ctcgcggtgg tggatgcggt tgacgaacga gctgttggcg ccgttctcga
     2821 gcaatctgcg caccagatag ggcagcagct cctcgtggct gccgacgggc gcgtagaccc
     2881 ggcacgccac cgccaccttc ccgtcctccg cgaggtggcg gtaaagcgcg tctcccattc
     2941 cgaacaggcg ctggaactcg aacggccggt cgcggccggc gagctccacg atcgccgcga
     3001 cggtttgcac gttatggctc gcgaactggg cgaagaacgc gtccgcatgg gccaggatcg
     3061 cgcgcgcgca ggcaaggtag gagacgtcgg tcgccgcctt gcgcgtaaac accgggtagc
     3121 cgtcgagccc gcgctcctgg gcgcgcttga tctcggtatc ccagtacgcc cccttgacca
     3181 ggcgcaccat cagccgccgt cgcgtgcgcc gggcgaggtc ggcgagccag tcgatcagcg
     3241 gccgggcgcg cttctgatag gcctggactg cgacgccgag cccgtcccag ccggcaagct
     3301 tcggcgccga tgcgagcgcc tcgaccacgt cgagcgacgg ctcaagccgg tcggcctcct
     3361 cggcatcgac ggtcaggccg aggcccgccg cggccgcggg ctcggccaaa gtcaccagac
     3421 gcggcaccag ctcggccatg acccggcgcg actgggcgaa ctcgtagcgc gggtgcagcg
     3481 ccgacagctt taccgacagc ccggggccct cgaccgggcc gcggccgccg gccgacgcgc
     3541 cgaccgcctc gaccgcacgc agataggcct cgaagtagcg ttcggcgtcg gctgcggtac
     3601 acgccgcctc tcccagcatg tcgaacgaga agcgtgtgtc cagcgccggc gcctcgtccg
     3661 cctgcgcgag cgcctcctcg atggtccgcc caatcacgaa ctggcgggcg aggatccgca
     3721 tcgcccgggt caccgcggcg cggatcactg gctcgcttga gcgggcgagg aagcgcgcga
     3781 gcacggcacc gagatggttc tcgtcgcccg cctccaggcg caccacgcgc ccggtcagca
     3841 tcaggcccca ggtcgaggcg ttgacgaaca gcgactcgcc ctcgcggaat ttgcggtgcc
     3901 agtcgacgcc gccgatcttg tcgcggatca gcctgtccac cgtgtcggca tccgggatcc
     3961 tgagcagtgc ctcggccagg cacatcagcg ccaccccttc gcggctggtc aaatcgtagg
     4021 cgcggaggaa ggagtcgatg ccgccatggc cgtcctcgtc ggcgcgcacc gcgccggcca
     4081 gccggtaggc gcgatcgtgg atgcgcttgg cggtggtcac gggcagggcg gcttcgcgcg
     4141 ccaggctacg cacgcacgcg gtctcgtcgg cgcgcgtcgc ggcgcgcacc gcaaggcgcg
     4201 acggcgaggg gggcgtcgct gccggcgcca ggatcacggc cggtccgggt ttccttccgc
     4261 gggtcgcgtc gccgcgccgg cgaagaacgc tcgcgcctgg tcatgatggt gcggcttcag
     4321 gtggccgccc accgccgaca gcgcggtcag cagcaccgag gcgtcgtcgg tgaatccgag
     4381 ccccgcgatc acgtccggga tcacgtcggc gggcacgatg aagtaggcga gggcgccgat
     4441 cagcaccgca cggacgtagg tcggggtttg cgggtcggcg gcgcaataga acgccgccac
     4501 cgcgtcgtcg gcgaacggga tgcgccccag cgcgcgcttg agcttgcgcc agaatccgtc
     4561 ccggaccagg cgctcctcgc gggcgagctt ggccgcatcc accggcagcc cggcaaggcc
     4621 cacgacggaa tcggacggtt ccgagtccat ggcctcaaat tgggcggcgt tcgaccgttg
     4681 cgcaagacgg cgcgctcagg ccggcgccgc cgccggctcc ggaatcgtga agccgtgcac
     4741 ttcggcgagc gccttgccgg actgaatggc ggcgatccag ccggcctcgg tcgcgcgctt
     4801 ggcctcggcg cgggcgatca ccgcatcgac ctcggtgcgc ggaatcacca ccacgccgtc
     4861 gtcgtcgccg accaccacgt cgcccggcag cacgcgcgcc ccggcgagca ggatcgggcc
     4921 gtcgatctcg ccgcgcgggt cgcggccggc gccgtgcggg gtgacgccgc ggctgaagat
     4981 cgcgaggccg ttctcgcgca gctccgcgat gtcgcgcacc gcgccgtcga ccacgccgcc
     5041 ggccaggccg tgggcctttg cgtccagcgc catgatgccg ccgaggctgg catgcgcctg
     5101 gccgccgccg ccgtcgatcg ccagcacctc gcccggccgc gcgatcgcga tcgcgcacac
     5161 cgccgggcgc acggagcctg ggcagaacac ggtgcgcgcc tgtccgacca gcttcagccc
     5221 cgccttcacc gggcgcagcg ccgccaccgt ctggcgcccg tccatggcgt ccgacaacag
     5281 cgtcgtgtcg agcgcgccga gcgcccggcg ctgggtctcg gtgatcggct cgaagtcgat
     5341 cggatggcgc aggatggtca tggcggcact cctttccggt cagtctgacg gcgtctcgac
     5401 ggtcggcata acagcctgaa ccgcgccggc cggcaacctt tgagcgcgcg cggcaatgcg
     5461 tctataacaa atccgtcatg tccgccgtag aggaacgcca agacgacacc atcccggtgc
     5521 gcccggccca ccgcttcgac gaggcggcgc tggccgacta tctcggcggc cggctggacg
     5581 gcgcgcgcgg cgcgctccag gtccgccaga tgcagggcgg ccagtccaac ccgacctacg
     5641 tcatccgctt cgcgcgccac gagtacgtcc tgcgcaagaa gccgccgggc aagctgctgc
     5701 cgtcggcgca cgccgtcgac cgcgagtacc ggatccagaa ggcgctcgcc ggcagcggcc
     5761 tgccgcttgc cgagatgcgg ctctattgcg acgacgacgc ggtgatcggc accccgttct
     5821 acgtcatgga gcggatgcgc gggcgcgtgt tccacgaagt caccgtgccg ggcgcgccgc
     5881 cggaccatcg cgccgagatc tacgaccaga tgaacaagtg cctcgccatc cttcacggcg
     5941 tcgactggca ggcgcgcggg ctcgccgact tcggcaagcc gggcagctat ttcgcccgtc
     6001 aggtgtcgcg ctggaccaag cagtggcatg cctcgaagca gcgcgagatc gaggcgatgg
     6061 accgcctgat cgaatggttg cccgagcatc ttccggacga cgacgagacc acggtggttc
     6121 acggcgactt ccggctcggc aacctgatga tccacccgac ccggcccgag gtgatcgcgg
     6181 tgttcgactg ggagctgtcg accctcggcc atccgctcgc cgatgtcggc tacaacctga
     6241 tgccctacgt caccccgcac gacgcccatc tcgggctcga gggcgccgat ctcgacgccc
     6301 tcggcattcc ggacatggac acctatctcg cggcttattg ccggcgcgtc ggccgccctg
     6361 tgttcgatcc gaccttccac atcgtgttct cgctgttccg catcgccgcc atcgtcgagg
     6421 gcgtgatggc gcgaggcgtc gccggcaacg ccagcagcga ggacgccgcg cggtacggcg
     6481 cgctcacccg gccctatgcc gagcgcggct gggcgatggt cacgcgcgcc gggctcgcct
     6541 gacatgccct ggcccgacga ggtgcgcctg ccgatgcgcc gcgaggcgca tttcggcgac
     6601 cgcgtggtgc gctgcttcgc cgatcgccac aacgacatcg accagctggt ccgcgccgta
     6661 gtcgcgcgcg accccgatgc cgaggcgctg gtcctcgacc ggacgcggct cagctatggc
     6721 gcgctcgacg accaggtggc gcgggtcgcc gccaacctcg cgcggcgctg cggcgtcggc
     6781 ggcggcgatc gggtggcgct gttctcgggc aactgcatcg agttcgcgct cgcggttctc
     6841 gcctgcgccc ggctcggcgc ggtgtcggtg ccgctcggcc accggctgca gacgcccgag
     6901 ctcgcctaca tgatcggcca ttcgggcgcc aaggtggttg cggtcgaggc gcagctcgcc
     6961 gagcgcctgc cggcgcgcgc cgcgatgccc gagatgcgcc atctgctcgc gttcgagggc
     7021 gacgccccgg gctgcgcgcc gttcgccgag ctgctcgccg acgcgcccgc gccgccgcgc
     7081 cccgacatcg acgaggagca ggttgcctgc atcctctaca cctcgggcac caccggccgg
     7141 ccgaaaggcg ccatgctcac ccacgtcaac atcatccaca ccatgctcca ctaccagctg
     7201 tgcatggacc tgagcgcgcg cgagcatcgc accctgatgg cggtgccgat cacccatgtg
     7261 accgggctgg tcgcgcagct gttgtgcatg atcggcatcg gcggcacgac ggtgctgatg
     7321 cgcgcgttca aggcgcgtgc cgcgctcgag ctgatttcgg ccgagcgcat cacccacggg
     7381 gtgatggtgc cggcgatgta caagctgatg ctgatggacc cggagttcga ccgcttcgac
     7441 ctgtcgagcc tgcgcgtcgg cggcttcggc ggcgcgccga tgcccgaggc gacgatcgag
     7501 gagctcgcgc gccgtgcgcc gtcggtcaag ctgcgcaacg cctacggcgc caccgagacc
     7561 acctcgcccg cgaccctgct gccgatcggc aaggcggcga cccacaagca cacggttggc
     7621 cttccattgc cttgcgccga cttgatcgtg gtcgatgagg acggccggga gctgccgcgc
     7681 ggcgccaccg gcgagatatg gatcgcgggt cccatggtgg tccccggcta ttggcgcaac
     7741 gaggacgcca acgccagcga gttcaccggc ggctattggc attcgggcga catcggccgc
     7801 ttcaccgagg acggctacct gcagatcctc gaccgggcca aggacatgat caaccggggc
     7861 ggctacaagg tgttctcggc cgaggtcgag aacgtgctcg tccagcatcc ggcggtggtc
     7921 gagtgcgccg tggtcgctcg tcccgacccg gtcctgggcg aacgcgtcca cgccttcgtg
     7981 ctcgccgatg gcgaggtcaa cgccgatggc ctgcgatcgt tctgcggcga acgcttggcc
     8041 gactacaaga tacccgagat cgtgacgttt accgaccagc cgctgccgcg caacgccaac
     8101 ggcaaggtgg tcaagtccga gctgcgcgcg cgcgccgtcg cgctggcggc cgaatggggc
     8161 gaggaggcga cggcatgacc cgtctgttga cgatcggatc gatcctgctg tgcgccgcgt
     8221 ccgcggccca cgccgccgac ctggacgcgc tgagcgcgcc cgacgcttac gccgggctct
     8281 gagccagcac cggtatatgc tccgtggagc ggatgtggac gtcgcgttgc gggaacggga
     8341 tctcgatgcc gttggcgtgg aacttgtccc agatcagcag aagcacgtcg cttttcacgt
     8401 tcgatacgcc gttgcgcgga tcgttgatcc agattcggag ctcgaggtcg accgagctgt
     8461 cgccgaagcc cttgaccagg cataccgatt gcggcgtccg gttcacccgc ggagcctcag
     8521 cggcggcttc gaggcaaagc gcgatcgcct tgcgcaggtc cgacttgtac gagatgccga
     8581 ccgggatctt cagccgcacc gcgtcgttgc tgtacgacca gttgaccacc tgctgggtga
     8641 tgatgtcctc gttggggatc aggtactcgg tgccgtcgcg ggtcacgacc gaggcgtagc
     8701 gggcgccgag cgtgctgatc cagccgtagg tctggccgac ctcgatcacg tctcccggct
     8761 tgaccgactt gtcgagcagc aggatcaccc cgctgatcag gttggacacc accttttgca
     8821 ggccgaaacc gacgccgacg ccgaccgcgc cgctgaacac cgcgaacgcg gtaaggtcga
     8881 tgccgacggt attgagcgcg gcgacgatgg cgatcgtgat cagcacgatc ttgagcagct
     8941 tgctgaacag cacctgcacc gccggggtca ggttcggcag gccgccgatg cgccgctcga
     9001 gcagccgtga cagcagcgac gcgccccaca gcagcaccgc cagcgatacc atccccttga
     9061 tcacggtcag caccgagatc cgcaacccgc ccagcgtgat cgccagcgcg tcgagcaggt
     9121 ccatggtctc gtccaggagg tcgaggatgt tgagggcggc caatgtccag acgacaagcg
     9181 cgaccagctt cgaccaggtc gggtcgcgca ccagctggga cgcgaaccgg atcacgaccc
     9241 aggcggtgag caggctgacc gcgatccgga tcaggtcgct gggccagccg gcgcgcgccg
     9301 cgatcagcac cgacagccat tgcagcacca gccagatgat cggctcggac atggtcgccg
     9361 ccgccatgcc cgcgcgcgtc aggcccagct cgagcttgcg ccggctcgcc agccgctcga
     9421 gccagcgctg gagacggggc gcgaaaaaac gggcgagaac gaaggccgcc agcacgaacg
     9481 ccgcctgcac gaccgtgctg gggaccagca cgtcctcgaa cagccaagcc tcgatgtccc
     9541 ggtacgcgga ctgcaaggtc gagaggtccg tcagctgctc catgcgatcc tcccctcgga
     9601 gcggacccgc ggtttcaaat ccacctcagg cgtctgagaa tcagcacctc ggcgacggcg
     9661 atcatgacca ggaccgtgac cacggccggg aacgcccatg ggctttcgat gcccggcatg
     9721 ccgccgacat tgatcccgaa caggccggtg atcaggctcg gcggcagcag caccgccgcg
     9781 accaccgtca gcaggtacat ggtccggttc atctgctcgc tcagccgggt gctgagctcg
     9841 tccatcgtca ccgcggcgcg ctcgcgcgcg gtgtccaggt cctcgacgta gcgcaacgtg
     9901 cggtcggcga cctcgcgcgg gcgcacgtcg tcctgcagca aggtctggca caccaccgcg
     9961 tcgagcccac tcgagtcggc cagccattgc ctggtcttcg ccccggtccg gtcgagatgc
    10021 acccacaata tcccttgatc gggtgcccag gcctcgattt ccggccagcc gatctcgcgg
    10081 ccgccacccc ggccatccag gattgccgcg gcgatcagcc cgtcctgttc gctcatggcg
    10141 ccgtcctctc tgcttgccct cgccgctagg tgccctggaa gctgggcttg cgcttctcca
    10201 gaaaggccgc cacgccttcg gcgaaatccc gggtggccga gcaggccgcg atgttctgcg
    10261 cctcggcgtc gagctggtcg gcaaggtccg agccgagcga gcggttgagc agggccttcg
    10321 cccggccgta ggcgagggtc gggccggcgg cgaggcgctc cgcgagcgtc gcggtttccg
    10381 cctcgagcgc cgattcgggc accaccttgt tgaccagccc gagctctgcg gccttggccg
    10441 cgtcgaacct gtccgccagc agcaccagct ccatcgcctt gtgcatgccc accagccgcg
    10501 gcaggaaaaa ggtcgaagag ccgtccgggc tggccccgat cagcgagtag gcaagggtga
    10561 agaaggcggt gtcggcggcg atcaccaggt cgcaggccat cgcggtcgag aacccggcgc
    10621 cggcacacgc gccgtggacg ctcgcgatca ccggcttggc catccggcgc atgctgacga
    10681 tggcgtcgtg gaaggcgtgg gtaatgtcca ggatcacccg gtcgagatcc gcgcccatgt
    10741 gctcgcggaa cacgttcagg tcgccgccgg ccatgaagcc ggcgccggcc cccctcagca
    10801 ccacggcgcg gaccgcgcca tcgagctcga gcgcgagcat ggtctcgcgc aacgcctcgc
    10861 cgagtacgcg gtcgatggcg ttgagccgct cgggccggtt gagggtcacg gtcgcgatcg
    10921 cgccgcggcg ctcgacaagg acggggctgg tcatcacgct cctcatgatc gactcgcggg
    10981 gtccgtgccc taatgtgacg gcaaacgctc ggcaaaggaa agtgccccgg ccggtcgggg
    11041 gcgcgcgcgg tcgaatcggt tgcgaaagcc gtgctagggt gctgagggtc agcagcaggg
    11101 gagtccggtc atgccgcgct ttctgaagac cgggatcagc ggcgaggaac gcgccgccgc
    11161 cgacgacaag gtgcgcgccg tggtcgaggc cgcgctcgac gacatcgccc ggcgcggcga
    11221 tgacgcggtg cgcgagatgt cgatcaagtt cgaccgctgg gagcgcgagg acttccgctt
    11281 gagcgagcag gagatcgagg cgtgtctcgc cgcggtcgag ccccgcgacc gcgccgatat
    11341 cgagttcgcc caggcccaga tccgcaactt cgcccgcatc cagagggacg cgctcaagga
    11401 cgtcgaggtc gagaccctgc ccggagtcgt cctcggccac cgcaacatcc cggtcaactc
    11461 ggtcggctcc tacgtccccg gcggcaagta cccgatggtg gcgtccgcgc acatgtcggt
    11521 ggtcaccgcc aaggtcgcgg gcgttgcgcg catcgccacc tgcgcgccgc cctatcaagg
    11581 cgccccggcg gcggcgatcg tcgccgcgca gcatctcggc ggcgccgacg agatctacgc
    11641 gctcggcggc atccaggcgg tcggcgcgat ggcgctcggc accgaatcga tcccggcggt
    11701 cgacatgctg gtcggccccg gcaacgccta tgtcgccgag gccaagcgcc agctctacgg
    11761 ccgggtcggc atcgacctgt tcgccggacc gaccgagacc ctggtgatcg ccgacgagac
    11821 cgtcgacgcc gagctgtgcg ccgccgacct gctcggacag gccgagcacg ggccgacctc
    11881 gccggtgatc ctgctcacca cctcggacgc gctcgcacgc gccaccatcg ccgaggtcga
    11941 gcgccagctc gccgtgctgg cgaccgccga gaccgccggc caggcgtggc gcgactacgg
    12001 ccaggtgatc gtctgcgacg acgacgacga gatggtccgc gtcgccgacg agatcgcctc
    12061 cgagcacgtc caggtgatga cccgcgaccc cgactacttc ctcgaccaca tgaccaacta
    12121 cggcgcgctg ttcctggggc cgcggaccaa cgtcgcctac ggcgacaagg tgatcggtac
    12181 caaccacacg ctgccgacgc gcaaggcggc gcgttacacc ggcgggctgt gggtcggcaa
    12241 gttcctcaag acctgcacct accagcgggt gctcaccgac gaggcctcgg tgcgggtcgg
    12301 cgaggtgtgc tccaggctgt gcgcgctcga gggtttcgcc ggccacaagg agcaagccga
    12361 catccgagtc cgccgctacg ccggcagcaa cgtcgccgga gagcagacgc gatgaccgag
    12421 cccgtgccgc acacgccgtc cttcgccctc gacggcaggc gggccctggt taccggcgcc
    12481 gggcgcggca tcggccgggc gtcggcgatc gcgctcgccg gcgccggcgc ccatgtcgtg
    12541 ctcgcctcgc gcaccgcctc cgacctcgag gcggtggccg gcgagatcga gggtgccggc
    12601 ggcagcgccg agaccgccgt gttcgacgtc accgacctcg acgccgcgcg cgaagcgatc
    12661 gcgcgcatcg gtccggtcca ggtgctggtc aacaacgccg gcaccaaccg cccggcgtcg
    12721 ttgctcgacg tcaccgtcga ggacttcgac gcggtcatgt cgctcaacgt ccgggcggcc
    12781 ttcttctgcg tccaggcggt ggcgcgcggc atgatcgagg ccggccgtgg cggctcgatc
    12841 atcaacgtct cttcgcagat ggggctggtc gcgcttcccg gccgctccgt ctattgcgcc
    12901 agcaagcacg cggtcgaggg catgaccaag gcgatggcgc tcgacctcgc tccgcacggc
    12961 atccgggtca acaccttgtc gccgaccttc atcgagacgc cgatgacccg cccgttcttc
    13021 cagaacaaga cgttcctgga catggtgatg gcgcggctgc cggccgggcg gctgggccag
    13081 gtcagcgacc tgatgggggc ggtggtgttc ctggccggcg atgcctccgc cctgatgacc
    13141 ggtaccagcc tcaccgtcga cggcggctgg accgcgcagt agctcagcgc gccgccagcc
    13201 gcaccgcgcc gtcgagccgg atcacggcgc cgttgagcat gtcgttgcga tagatgcagg
    13261 cgaccaggtc ggcatactcc gccggcgcgc cgaggcgctt gggaaacggt gtcgaggcgg
    13321 cgagcgaggc ctgcacctcc tccggcagcc cggacatcat cggagtcaag aacagcccgg
    13381 gggcaatggt catcacccgg atgccgaact gcgccagctc gcgtgcggcc ggcagggtca
    13441 gcccgaccac gccgcccttc gacgacgcgt aagccgcctg gccgacctgg ccctcatagg
    13501 ccgcgaccga ggcggtgttg acgatcaccc cgcggccgcc gtccgcgtcc ggctggttgt
    13561 gctgcatccg ctcggccgcc gccttcatca ggttgaaggt gccgatcagg ttgaccatga
    13621 tcaccgtctg gtaggtatcc agcgccagca cgcccgcacg ccccaggatt cgtcccggcg
    13681 tggccacccc ggcgcacgcg accgcgagcg tcaccggccc gagctcggcg accgcgtcag
    13741 cgaccgcgcc ggtcatggcc gcctcgtcgg tgacatcgag ctcggcgaag cgcgccgtag
    13801 cgccaagctc cttcgccgcc tcctgcccgc gcgccgtatc gcggtcgaga atcgtgacct
    13861 tgccaccgtc ggcgacgacg cgctgggccg tcgcccggcc gagcccggac gcgccgccgg
    13921 tgatgatcgc gtggcaggta gtgatgtcca tggtgcaggt ctccgattgc tgcaacgggt
    13981 acgcgccccg gccgcacgaa ccggggctca gggcgtgaac tgctcgcgcg cgatccgttc
    14041 ctcgagattg tgctcggggt cgaacagcag atgcaggctg gtctccgggt cctggtggat
    14101 ctccacccgg gtggcgccgc gcacctcggt gtagtcggcg gtggcgctca ccggccgctg
    14161 gtcggcatcg agcacgtcga cggtgatgcg tgccgattcc ggcagcagcg cgccgcgcca
    14221 gcgccgcggc cggaacgcgc agatcggcgt cagcgccagc aggcgcgagc ccagcggcag
    14281 gatcggccca tgggccgaca ggttgtaggc ggtgctgccg gccggggtcg cgaccaggac
    14341 cccgtcgcag accagcagct ccagccgcac gcggccgtcg atcgacacgc gcaggtgggc
    14401 ggcgaaccgg gtctcgcgca gcagcgacac ctcgttgatg gcgagcccgt cgaccctgcc
    14461 gccgtcggcg cgggtcacgg tcatgcgcaa cggatggagc accgtcggct cggccgcggt
    14521 caggcgctcg accagcccct cggcccggta ctggttcatc aggaacccga ccgtgccccg
    14581 gttcatgccg tagatcggcg cgccgcgatc catgaactgg tgcagggtct cgatcatgaa
    14641 gccgtcgccg ccgagggcga cgacgacgtc ggcccgttcc ggcggcacgt ggtcataatg
    14701 cgcggtcagc gcctcatacg ccgccgccgc ctcggggatg tcggcggcaa cgaagccgat
    14761 cgccgggcca gccatcgtgt cgcttcgctc ggggttagga gaggcgggcg ccgccgagcc
    14821 cggctcagcc gccggtcatg ctcatgtggc ggccgaccgc cgggcgcctg tcgcgccggt
    14881 cgatgacgaa gtcgtggccc ttgggcttgc gcgagatcgc ctcttggatg gcggcgatga
    14941 gcggctcgtc gctctcgctc gcgcgcagcg gcgcgcgcag atcggccgcg tcctcctggc
    15001 ccaggcacat gaacagcgtg ccggtgcagg ttagccgcac ccgattgcac gattcgcaga
    15061 agttgtgggt cagcggggtg atgaagccga gccgccggcc ggtctcctcg acgcgcacat
    15121 agcgtgccgg cccgccgctg gtgtagtcga tgtcggtcag cgtccaacgt tcgcgcagcc
    15181 gccggcgcac caccgacagc ggcaggtact gttcggtgcg gtcgccggtg atgtcgccca
    15241 tcggcatggt ctcgatcagg gtcaggtcgt agccgcgctc gccgcaccag gcgatcaggt
    15301 cgtcgaactc gtgctcgttg acgcccttga gcgcgaccgc gttgaccttg accgcgagcc
    15361 ccgcggcatc ggccgcctcg aggcccgcca tcacccgctc gagcttgccc cagcgggtga
    15421 tcgccaggaa cttgtccgca tccaaggtat cgagcgagac gttgacccgg cgcacgccgc
    15481 aggcgcgcag ctcgtcggca tagcgtgcga gctggctggc gttggtggtc aaggtcagct
    15541 cgtcgaggtc gccgctgcgg acgtgccggc cgaggctccg gaacaggctc atgacgttct
    15601 tgcgcaccag cggctcgccg ccggtgagcc gcagcttgcg cacgccgagg cggatgaagg
    15661 cggtgcacag gcggtcgagc tcctcgaggc tgagcacctc ggacttgggc aggaaggtca
    15721 tgtcctccgc catgcagtag acgcagcgga agtcgcagcg gtcggtgacc gatacccgca
    15781 gatagctgat cctgcgaccg aaggggtcga tcatgtcgcc ctgtgcctcc caagcccgcc
    15841 tgctgcgccg ggcaagcctc aaccggcgcg gcgacggtta tatcttgcgg tatataaagc
    15901 atccggggcc ggcgaacaac acgccttctg gcccgcgctc cggctacctg cgctcgccct
    15961 cgtcctcgac cacctcgagc gccaccaccg cgacgttgat cagcaccttg ccggcgtgct
    16021 cgaaattcac cgtcacccgg ttgccgatcg cggtctggac ctggcccaat ccccagtcgg
    16081 gctggcccgg atggcggacc agagaccccg gcaccaggcc cggatcgcgc atggtcattc
    16141 ctggatccga tctggacgga ccggcgcccg caccagcgcg ggcgcgcccg gaggctatct
    16201 tatggacgcc cggccggtcg agggcgccgt agacgaaggc tcaaacggcc gccgccgcgc
    16261 tcgaggcgtc ggcaatggcc gaccgcagcg acgcatcgtc cggatccttg agcatatagc
    16321 cacgtcccca cacggtctgg atgtactggt ggccggaaat cgcgaccagc ttcttgcgca
    16381 gcttgcacac gaacacgtcg atgatcttga gctcgggctc gtccatgccg ccgtaaaggt
    16441 ggttgaggaa cgcctccttg gacagggtcg cgcccttgcg cagcgccagc agctccagaa
    16501 tcccgtattc cttcgcggtc aggtggacgg actggccggc cacctcgacc gtccgctggt
    16561 ccaggttgac cgcaaggtcg ccggcctgga tcaccgagtc ggcgtggcct ttcgaccggc
    16621 gcacgatcgc ctggatgcgg gcgagcagtt cggcccggtc gaacggcttg gtcaggtagt
    16681 cgtcggcgcc ggcggacagc ccgcgcacct tgtggtcgat ctcgcccagg ctcgacagga
    16741 tcagcaccgg agtggtcacc ttggcgtcac gcagcgcgcg caccacctcg tgaccgtcga
    16801 gatccggcag gatcaggtcg agcaggatga tatcgaagtc atagagcttg gcgagctcga
    16861 tggcgtcctc gccgaggtcg gtcacctcgc agaccatgcc ctcggacttg agcaccgcct
    16921 cggtgaccct cgccgtggcc ggatcgtctt cgacaagtag gacccgcatt gcacgcttcc
    16981 cctgcaatcg cggcgggcca aatatccccg cttattaacg gatatagatt taagatccgt
    17041 gccggcaaac gcgttcaccg atttttcagc aatcctgggt tatcaggcgg aatcccggac
    17101 ctgcgcaggg tcttgccggc gccggatcag gcgtgcgtgc ccgccgttaa cggattggcg
    17161 attgacgcac atgcgcctgc ccgcaatttc gttggggcca gatgaccgac cgcttcttca
    17221 ccgacctcgc ccacgagatc gaccggctcc ccgacctgcg agtcagcggc cgcgtggcag
    17281 gcgtccgtgg cttgctggtc gaatgcgacg ggcttcaagg cgtgctcggc attggcggcc
    17341 gatgcgcgat catcggccga tcaaagcaac ctataatatg tgaatctgtc gggttccgca
    17401 atgatcgcgt gttagtcatg ccatttgcgc cactggaagg cgtcgccctc ggcagcgagg
    17461 tcgccgtcat cgaggcgcag gcctcggtgc atcccgagat gcgctggctc ggccgcgtgg
    17521 tcaacgcccg cggcgagccg ctcgatggca aagggccgcc cggcatcggg ccgcgggcct
    17581 atccgctgcg cgcccggccg ccgcccgccc acagccgccg gcggatccgc ggcaaggtcg
    17641 acttgggggt gcgcgcgctg aacaccttca tcacctgctg ccgcgggcag cggatgggca
    17701 tcttctcggc ctcgggcgtc ggcaagtcgg tgctgctgtc gatgatggcg cgctacacct
    17761 cggccgacgt gaccgtgatc ggcctggtcg gcgaacgcgg ccgcgaggtc caggagttca
    17821 ttcaggacaa cctcggcccc gacgggctcg cgcgcagcgt ggtcgtggtc gcgacctccg
    17881 acgaatcggc gctgatgcgc cgccaggccg cctatctgac cctgacctgc gccgagtatt
    17941 tccgcgacca gggcctcgac gtgctgtgcc tgatggacag cgtcacccgc ttcgccatgg
    18001 cccaacgcga gatcggcctt gccagcggcg agccgccggc gagcaagggc tatacgccgt
    18061 cggtcttttc cgagctgccg cggctgctgg agcgggccgg gccgggcgtg ggcaagggct
    18121 cgatcaccgg cctgttcgcg gtcctggtcg agggcgacga ccacgacgag ccgatcgccg
    18181 acgccgtgcg cggcatcctc gacggccata tcgtcatgga gcgcgccatc gccgagcgcg
    18241 gccggtttcc ggcgatcaac gtgctgcgca gcatctcgcg caccatgccc aactgcaaca
    18301 gcgcggccga gaacgccttg gtcacccgcg cccgggcgct gatggcggcc tatgccgaca
    18361 tggaggacat gatcaggctc ggcgcctatc gccgcggcag cgacgccctg atcgacgagg
    18421 cgatcgacta ccgcgagcct ctggagcgct tcctcgccca gggcatggat gagcgcagca
    18481 gtctcgagga cggctacgcc gcgcttgccg gattgctggc gaagtgagcc gtgaccggtg
    18541 cgcgcgctcg atacgctgat acgaatgcgt cgcgatgcgc tgctcgatcg gcgccgggtg
    18601 ctggtcgagc tcgatgccgg gcgcgcggtc gtcgtccgcc ggctcgcggc gctggccgcc
    18661 gcgctcgacg atgagcagcg gatcgccgcc ggctcgctcg aggcgctgta cgcctaccgc
    18721 ggcttcgccg agcaagtgat cgccgagcgg gcgcagctcg agcgctcgat gcaggagctg
    18781 gacgcgcgca tcgccaaggc ccgcgaggac ctggcggcgg cattcagcga gctcaagaag
    18841 ttcgagatcg cccgcgatca gcggctgcgc cgggagcgcc aggcgcgcgc gcaggccgag
    18901 cagaaagcgc tcgacgagct tgccctggcg cgctaccgcc acggccgctt cgaccccacg
    18961 cagagccgcc gctagggcgt ttcccggccg cggcggggcg cccgcgttcc gcaccgcccc
    19021 aacggactcc tagtcaagcg cgatccgtga cagcatcagc cggtcctgcc agtcgggctt
    19081 gcgctgctcg aaggtcaccc ggtgggtggt cgctccggcg gcctcgagcc tgagctgcac
    19141 ccagcgcggc agcagctcgc tgtccaggtc gccgattatc tcggcgccga gcgtctcgag
    19201 cgagtcccag tcgagcgtac cgatcgcggc cagataggcg ctgagccccg cttgcggcag
    19261 gaagtgccgg tcggggacgt agcggatcga gatggagatc ggtcccggca acgatgtgcc
    19321 ggcaaatgtt tcgcttagcg taatcacgca gtcgagcccc ggccgcgggt tgtcggcgac
    19381 ggccagatgg ctgcggcgca gccgtgtggt ctcgaaccgg tcatccatga tgcgaagtca
    19441 cgtcgcgacg ctatcgggcc ggccgagcgc gctcactatc tggaactcga tcgcgccggc
    19501 gaggcccgag accgccaaga cgtcatcgaa gatcatgcgc aggttgcggc gcgccgcctg
    19561 gtccagcggg cgcaggctgc gcagcaccac gtcgaaccgc cgcttgcggt acagcccgtc
    19621 gatgcgcaac cggcccaggc ggctcaggtc cacgtcgacc accagccggg tgccggcctc
    19681 ggcgccgtcc tccgccgcat cggcttcgcc gcgctcgcgc ccgaaccaga gcctggcctc
    19741 gtgaatctgc ccgtcgccct gccagggcag ggggatctgc cgccagtcgg gcgcaccggc
    19801 ggcatcgccg ccgcgccagg cgtggcggaa gtcctcgccc agccgctcga ccagctgccc
    19861 gcccgcggcc cgctccaggg tccgcgctct ggcctcgccc aaccacaggc gcgcatcgcc
    19921 cgcgcgcagg ccgagcaaat aggcgatcaa gcctaagacg gcagacggcg cgcctccggg
    19981 aatctgcgcc atcaccgccg cgcgctctcc ggcccccgcg gccgccatcc gcgcaagagt
    20041 ctcgatgacc ggcgatctcg ccgcagggtc ggcaatcggt gcggtcgagg ccgccggcag
    20101 catctcgaag gtaagcgcgg tccccggcgg cagcggcgca tcgctcggca gcgccacggt
    20161 ccgctccgcc atgtcgacca gcagccggcc gtcggcgtcg cggccgagga cggtcgccga
    20221 gacctcctgc gcgcccgcgg tctccggcgt ggagggcccg aacccgacca ggcgcagggc
    20281 gaccgtcgca cccttgggcg cagccgaaag gaccattggg gtcgtcaccg gcccacgccg
    20341 ccgcggcgtc gcgacctcga ccacggtgcc ggtgaccgtg cgcccgggaa tgagcacccc
    20401 gcgcgcgccg gtcgcggcga ttgcctgcgc ccgcgcccgc gccagcgccg ggccttgcgc
    20461 gggggcgagc gccaggcgcg ccggcgcgcc gcgcgccggc gaaacctgtt gcgcgatgat
    20521 gtgacgggcg cgggcgacga tctcgagaac gagcctggtg ccgctgtgcg gcagcgccgc
    20581 cggcgccggg gcctcgagcc ggaccacgcc ggcgtcgccg accagcaggg cggtgccggc
    20641 agcgggcagg ggtacgaccg tgccgatgac cactgccccg ggcggcagct cggcgaaggc
    20701 cgccggcagc gtgcccgccg tctcgccgcc aggcacggca tggggtgcga gcacgatcag
    20761 ggccgtccca ccggctccga ccggaaccgc aagcagcgcg tcctcggcgg cgacgacctc
    20821 gagcaccgcg tgcgcggcgg ggccggtcgg ggttgccgcc aacaccgccg catcccccca
    20881 ggccgccctg aacagggtgg tcgtgccgct cgagtcgacc tcgatcgctc cgtcgaggcg
    20941 tgtgcccggt gccgccgtcg ccaacgcctc cggcggcgac agcggcgccg tcacaggtgc
    21001 cgggcccgcg cgcgcacccg ccgcgagcgt gtcgaccggc cggtcggcgc gcggcgccgg
    21061 atcgcggctc gatggcgcgg cacccggtgt cggcgcggcc gtcggcgtgc ggccgtccgc
    21121 ggccggggcc ggggcgcccg ccgccggcgg cggcagcggc ggcaggccgg cgctcatgtc
    21181 acgtgttatc cgccagcgtg gcggcaatcc gggccacgtc ggcgaccgag tcagacgtcg
    21241 gagagcggct gaacaccgag acctggcggc gaatcgcgtc gcgcacccga tcgtcgcggc
    21301 gcaccacgcc ggcgagcggc ggcgcgatct tgaggaacgt ctcgcacgcc ttcaccagcg
    21361 tcgcgtgggt ccgttcgccc tcggcttgcg aggcggcctg gttgaccacc accttcaccc
    21421 tgcggccatg tccggcggcg gtcagcacct tgatcagggc gtaagcgtcg gtgagcgcgg
    21481 tcggctcgtc ggtgaccagc accaggcagg tgcccgacat gccggccagc aggcgcaggt
    21541 tctcgtccag cccggcggca aggtcgagca ccaccttgtc gtagcgcgcg gcaagcgcga
    21601 tcaggccgtc gcgcaggctc ttgagccggt cgggtggcag cgccgccagg ctgcccgagc
    21661 cggagcggcc cggaagaatg tcgaagcccc cgtcctcgaa ccactggacc gcgtcggcga
    21721 acgtcacctt gccggcaatc accgccgaga tgtcgtactt gggcatcagg cccagctgga
    21781 cgtcgatgtt ggccagcccc aggtcgccgt cgaacagcag cagccgccgc ccgcgcgccg
    21841 ccaacgcgtg cgcgagcgtg atcgagaacc aggtcttgcc gacgccgccc ttgccggacg
    21901 cgaccgcgat gacgttctcg accaggtgcg cggcggcggg gtgcctggcg acattgcttg
    21961 gcgtgctcat cgtgctgctg ccttctcggg ctgggagttc gtggccgaag cgatcggttc
    22021 gcctaacaac agtctggtca gcgcgtacgg ggtaatcggg gtcaggccga gcgccacttg
    22081 cgcgctggcg ctcgcctcgg cgaacgcgaa cgggcccgcc tcggcaacca ccagcagcgc
    22141 gccgagccgc cgggcgacgt cgagcctggt cgcgaccagc cggtgcgcgc cgagcgcggc
    22201 gaagctgccg gcgatctcgg ccgcctcgag cgaatcgccg ccggcggcca tcaccagcac
    22261 aggctcgccg ccgcccgcct cgaccaggcg ggcgagggcg gccatgtcgg ccggatcgaa
    22321 cgggttgacc ccggcggtat cgatcaggac gacggcgtcc gatgcgccgg cggcgatggc
    22381 cgcgcgcagc tcgtccggcc catcggcgtc gaccagctcg gcgttcagga tgtcggtgaa
    22441 cgcggccagc tgcgcgcgcg cgccggcgcg aaaggtatcg gtgctgatca ctttcaccgc
    22501 gcacccgtcg atggtcgcgc gcgccgccag cttggcggtg gtcacggtct tgccggcgcc
    22561 gggcgtgccg accatcatca gcggccgcgc gcgcgggtcg agcttgaccg gctcgaacaa
    22621 gaacgcgtgc gcgagcgcgg cccgaagcag cgaggcgacg tcgtcctctc ctgcgtccgc
    22681 gaccagcccg cccgccagat cgtccggcac gccatgatag gcgagcgccc cgagggcctc
    22741 ggcgcgcgcg cccgacgccg gctcgtcgcc gatcagccgg gcgaggacgt cggcatcgtc
    22801 caggttctcc agcgccgcgg tgatccgcac ctcgccgtcg gtgtcggtct cggtcgagac
    22861 gattaccgcg tcgtcgccca gctcgttgcg gatctggcgc atggcatcgg cgatcgtgcg
    22921 ggcggtgtag actctcaggc gcatcgcgct gcgctcagac ctgtccgagg gtgtggatct
    22981 tcgctttcgg gtgaatctcg ttctgtgaca ttaccacggt cgaggggcgg aaccgctcga
    23041 taatggaccg gacatagggg cggatcgcgg ggctggtcaa cagcaccggc atctcgcctt
    23101 cgccggcgtg cttgtcgaac tcctggcgca ccgaggtgat gaactcctgc agcttgctcg
    23161 gcgccatgtg cagccgcttg tcgtcgttct ggccgaccag cgactcggcg aaggcctgct
    23221 cccattgggg ggccagcgtc accagcggca cgtagcccgc acgcgagctg ttgtcgtggc
    23281 tgagctggcg ggcaagccgt gcccgcacat gctcggtgat caaggtcacg ttctgggtgt
    23341 agcccagcgc ctccgaaatg ccctcgagga tggtcggcag gtcgcggatc gagacccgct
    23401 cggccagcag gcactggagc acgcgctgca ccccgccgag cgagatctgg cccgggatca
    23461 ggtcggcgac cagcttctgg tgctcggcgc cgagctcatc gagcagcttc tgggtctcgg
    23521 agtaggacaa cagctccgcc atgtggtcct tgaccacctc ggtcagatgg gtggtgatca
    23581 ccgtcgccgg gtcgaccacg gtgtagccgc ggaacagcgc ttcctcgcgc tggctttcgc
    23641 ccacccatct cgccggcagg ccgaaggtcg gctccctggt gtcctggccg ggcagcgcga
    23701 tcgcctcgcc gcgcgggtcc atgaccagca attggtgggg catcaggtcg cccactcccg
    23761 actcgatgtc cttgacccgg atcagatact ggttcgcggg cagctgcatg ttgtcctgga
    23821 tgcgcacggc cggcatgatg aagcccatgt cggtggcgag ctggcggcgc aacgccttga
    23881 tctggtcggt gagcctcggt cccttgtcgc tgttgatcag cggcagcagc ccgtagccca
    23941 gctcgagctt gagcacgtcg atgtgcagcg cggtggcgat cggctcctcc gcgggcctcg
    24001 ccatcgacgc ctcggcctgg gcccgcgcct gtgcatcgac ctcggccgag cgctgccgtg
    24061 cctgctgcga cagccgccac gcctggaagc cggtgagccc ggcgagcacc aggaacggcg
    24121 gcatcgggat gccgggcagc agcgacatcg ccaccatcag cgcgctgacc aggccgagcg
    24181 ccttcggata gctggcgagc tggccgaaca ccgccttgtc ggccgagccg accacgcccg
    24241 ccttcgacac cagcagcccg gcggcggtgg acacgaccag cgccggcatc tgcgacacca
    24301 ggccgtcgcc gacggtcagc acggtatagg tgttcgcggc atcggcgaag ctcaagccgt
    24361 gctgggcgac gccgatcacg atgccgccga tgatgttgat gaaggtgatc aacaggccgg
    24421 caatggcgtc gccgcgcacg aacttggcgg cgccgtccat ggcgccgaag aaggtgctct
    24481 cgtcctccag ctggcgccgg cgccggcgcg cctccgactc gtcgatcagg cccgacgaca
    24541 ggtcggcgtc gatcgccatc tgcttgcccg gcatcgagtc gaggctgaag cgcgcggaga
    24601 cctcggcgat gcgggtcgag cccttggtga tgacgacgaa gttcacgatc accaggatcg
    24661 cgaacacgat caggccgatg acgaagttgc cgctcatcac gaagtcgccg aacgcctgga
    24721 tcaccttgcc cgccgcggcg gtgccggtgt ggccgtcggc gaggatcagg cgggtcgagg
    24781 ccaggttgag cgccagccta agtatggtcg cgatcaacag gatggtcggg aacgagctga
    24841 actccaaggg ccgggcgatg aacagcgaca ccatcagcac cagcaccgag aaggtgatcg
    24901 agatcgccag gctgaagtcc agcagccaac gcggcatcgg catgatcagc acgatcagga
    24961 tcgtgatcac gcacagcgac agcgccacgt cgccctcgcg cagcgtcgcc gccaggcgcc
    25021 ggataacgcc gccgaggtcc ggcgcggccg gcggctcggc ggcggtcggt ccccggatct
    25081 gaggtccgct gtcggccatt cccgttgccc ttgtgccctg ctagaggccc gcggcgcgcg
    25141 cctcggcggc gggcggcggt accgccacgc cctcggcgtc gtaggccttc agcttgttgc
    25201 gcagcgtcct gatcgagatg ccgagaatgg tggctgcatg ggtacggttg ccgaggcaat
    25261 ggtcgagcgt gtcgatgatc aggtcgcgct cgaccgcggc cacggtccgc ccgaccagcc
    25321 gggatgcgcc gccggtgccg gcgacgcgcc cggcctcgcc gtcgatcgcc gctggtcccg
    25381 cgccgccgcc gagcatcatc gcctcgggcc cgatctcggg gcccgtggcc agcagcaccg
    25441 cccggtgcat gttgttctcg agctcgcgca cgttgcccgg ccaggcgtgg cgcagcagcc
    25501 gctcggccgc ggccttcgac aacggccgtg gcgccagccc gttggcctcg gcgtacttgc
    25561 cgatgaagtg ctcggcgagc agcaggatgt ccctggtgcg ctcgcgcagc ggcgggatgt
    25621 gcacggtgac gacgttgatc cggaagtaaa ggtcctcgcg gaagttgcgc gcggcgacct
    25681 gttgctgaag gtcgcgattg gtggtcgcca gcaggcggat gtcgatcttc accggacggg
    25741 tgccgccgac ccggtcgatc tcgcgttcct gaaggacgcg cagcagcttg gcctggagct
    25801 tgaggtccat ctcgccgatc tcgtcgagca gaagcgtgcc gccgtcggcg gcctcgaact
    25861 tgccgatgcg gcgggcgatg gcgccggtga acgcgccctt ctcgtggccg aacagctcgg
    25921 attcgagcag cgcctcgggg atcgcggcgc agttgaccga cacgaacggc tttgccgagc
    25981 ggcggctgcg ggcgtggatg tggcgcgcca ggatctcctt gccggtgccg gagtcgccgg
    26041 tgatcaggat cgaggcgtcg gacctggcga tccgctcggc gaggtcgagg acctcgcgca
    26101 tcgccgggtc ctcgaacacg atccggtggt tctcctcggt caccgcctcg agcacggccg
    26161 cgatcatgtc ggggtcgggg ggcaggggca ggtactcctt ggcgccggcc ttgatcgccg
    26221 ccaccgccgc gcggctgtcg ttgcccatgc cgcaggcgat caccggcacg ttgatgtgct
    26281 cggcggcgag gctccgggtc aattggccga catcgagcgc gacgtcgatc atcaccaggt
    26341 ccgcgccgcc gccggcgccg ctgcgcaggt tgtgcagggc gatggcgata tcgtccgcat
    26401 gcaccaccct ggcgccgcgc gcgaccgcga tcttgcaggc ttcgcccagc tggccggcca
    26461 gcgttccgat gatcagcaag cgcatctgtg tcgtgtcccc tcggccgggt gttggcgggt
    26521 caatcgaagt actgcacccg cttgggcggc ggcagcaggg tgttgagcag ggtctcgagc
    26581 cggcgcgggt tctcctggcg gccgatcgag gacgcgccca gcgtgtacac gttcatcacc
    26641 gacgcctcgt cgttcgagtt gcgctcgagc ttggcggcga gcggcgagaa caccatgttg
    26701 gcaagcacgg cgccgtaaag ggtggtcaag agggcgaccg ccatcgacgg gccgatgcta
    26761 ttcggatcgt cgagattgcc gagcatctgc accaggccga tcagggtgcc gatcaggccc
    26821 atcgccggcg ccacggtcgc cgcctggcgc aggatgccga cgccgcgctc gtggcgcgac
    26881 ttcatgccct tgatctcctg cttcatcatc cgctcgacgt cgtcacccgg ggtgccgtcg
    26941 accaccagcg acagcccctt ctggaggaac ggcacgttgt cgaggtccct gagcttttcc
    27001 tgcatgccga gcacgccgtt cttgcgcgcg aggtcggcga gctcgatcac ctgcaatgcc
    27061 gccgcgcccg gatcgatcgc cttgttgaac accgccttca ggatcaggcc ctggcccttc
    27121 atgatctcgc cgagcgagaa gctgaccgtg gtgacggcga aggtgccgcc gatcacgatc
    27181 aggaccgagg ggatgtcgat gaaggcgctg atcgatccgc cggtggcaat ggcggcgaca
    27241 accaggccga agccgatcac caggccgagc agcgttgcga tatccatggc gcgtacccgt
    27301 cagaaccggt ccgtcttgat gatctcggtc atggtcacgc cgagccggtc ctcgaccacc
    27361 acgacctcgc cgcgcgcgac cagccggttg ttgacataga tgtcgatcgc ctcgccgacc
    27421 ttgcgatcga gctcgaccac ggcgccgcgg cccagccgca tcagctggct gacctgcatg
    27481 gtcgccttgc ccagcaccgc cgagacctgg accgggatgt cgaacaccga ctcgaggtcg
    27541 gcggcggtgc gcgggccgtc gtcgctgccg gcggcggctg gcgccggcgc cccacccgat
    27601 ccgatttcct cgagctcgag gtcttcgttg tcagccatga tcgtctcctg aaggcgaagg
    27661 ctcgccggcc gcttgcggct cggccggcgc cggctcgtcc gtgggcgcgg tgtagcgctc
    27721 taggatcgcc tcgatccgcg cccataactc cgactcgagg cgccgcgcgt tgccgccggg
    27781 ccagtcgatg cgacactcgg cgccggtcag cgaggaatcg ccgacgacgg tcaccgcgat
    27841 gccgctggcc ggcggcttga gccgcgccac gagcgtgtcg gcaagggtcg ggtcgaccgc
    27901 gaccgtcacc tgctcgacgc cgtagatctc ggccaggcat tcgtcgacca ggcgctcgac
    27961 cacctcgagc tggccttcgt gctcgcgccg gccgatgatc ttggcggcga tcgcctgcgc
    28021 gagccgggcc gtgtcatggc gggtcagcag ccgctgctcg gctccgctct gggcgacggc
    28081 ttgcagccgc ttggccacgg tctcgagcag gctttcgatg cgcgcctcga tcgctgccgt
    28141 cgcctgtgcc cgtccggccg cgattccgga ctcgtagccg ctctggcggg cgccggcgag
    28201 gtcgtcgtcg gagtatgcgg cgcgttcgcc ctcccgcgat tcgcccgcgc gccgcctggg
    28261 cgtgcgcccc tcggctccga aatcggtctc gaacaggaac ttgttgatct gggtcatgcc
    28321 tcgctcgcgc gcctcagtag accaactcgt cctcgccgct gccggcgata acgatgtcgc
    28381 cccgcgccgc catgtccttg gcggtgatga tgatctcctg ctgcgcctcc tcgacgtcgc
    28441 tgagccgcac cggaccgagc gcttccatgt cctcgcgcag gatcttggcc gcccgctcgg
    28501 acatgttggt gaagaacagg ttgcgcaggt cttccgaggc gcctttgagc gcgatgccca
    28561 ggcggtcctt gtcgatctcg cgcagcaggg tctggatgct gttgccttcg agcttcttga
    28621 ggtcctcgaa ggtgaacatc agggccttga tccgctcggc ggcgtcgtgg tcgcgatcct
    28681 cgagcgcctg caggaagcgg ctctccgagg cacgatcgaa gttgttgaag atctcggcca
    28741 tcagctcgtg gctgtcgcgc cgcgagctcg ccgccaggtt gctcatgaac tccgagcgca
    28801 gcgtgcgctc gacgtcctcc agcacgtcct tctggaccag ctccatgcgc agcatgcgca
    28861 tcaccacctc gaccccgaag tcgtcgggca acaggccgag gacgcgcgag gcatgcgccg
    28921 gcttcacctt cgacagcacc accgccacgg tctgcgggta ctcgttcttg agatagttgg
    28981 cgagcacctg ctcgttgacg ttgccgagct tgtcccacat cgtgcgcccc gcgggaccgc
    29041 gcagctcctc catgatctcc tcgacccgat cggtgtcgag cgtggcgcgc agcagccgtt
    29101 cggtcgattc gaacgagccc atcaggttgc cggtgccgga gatctggcgg acgaagtcga
    29161 ggaacaggtt ctcgaccacc ggcgcggcga tattgccgag ctgcgacatg atcgaggaca
    29221 gctccttgat ctcctccagg tccatcatgc cgaacagctt ggccgcccgt tcctcgtcca
    29281 gcgcgaacat gatgatcgcc gccttctggg gaccgcgcag ctcgcgcagt tcggtcttgg
    29341 tctgggccac gctcggcgtc ctcgcttgcc gctacaacgg ttgccctcag gtcgagcttt
    29401 cggccatcca gccgcgcagg atggacagcg cttcgtccgg gtgcttgtcg acgatctcgc
    29461 ccatcctctt gaccgccgag gccttgacct ggccctcgac cttattgatg tcgatcatgc
    29521 cctcgccatc cgcgcccgcg ccgctgccgc gcgcggccgg aagctccgcc cgggcctggc
    29581 cgccggcgcc gggctgggcg gcgccttcgg gcccggtcag catcggctgg ccgcgcggtc
    29641 ccgggatcgc gcccggcgca gtcgcggccg gctcgccggc gtgcgcctgc agcgtcagca
    29701 ggcggctgat caggggccgc agcaccagca ggatcaccag caccgcgacc agcgcgagaa
    29761 ccaggatctg ggcgatcctg aaatagtcgc ccttgtccag cccaagcagc ggctcgctcg
    29821 gcatctccac cggctcgggc tcggcgaagc gcatgttgat cacctcgatg gtgtcgccgc
    29881 gatcggcatc gaagccgatc gccgaacggg ccagtgccgc gatctgggca agctcgtcct
    29941 cggtgcgcgg cacgtagctc tgctcgccgc tctgcggatc ggtccgggtg ctgccgtcta
    30001 ccaggaccgc gaccgacagc cgttcgaccc gtccgccttc ctcgatgtgg ttgctgacgg
    30061 tgcgcgagat ctcgaagttg accgtctcct cggtgcgggc ggtggacgcc tggctgcccg
    30121 ggcccgcccc ggcctcgccg ccgggcaggt tgttggcgac cgagaccgac ccgggaccgc
    30181 tgccctcgct gtcggtggtc tcctcctcga ccgactggtt cgagcgcacc acttgcccgt
    30241 ccggatcgta gacctcggcg ttggtggtgg tgcggtcgaa gttgatctcg gcggcgacct
    30301 cggcgcgcac cttgccgggt ccgagcgatc gctcgagcag ctgctcgagg gaatccttga
    30361 cctgggcctc ataggcgtgg cggaactcat ccgcgcgggc ggccgcgaaa ccgatctcgt
    30421 tgcgctcctc tgcgccgctg gcgagcaggt tgccgcggtc gtcgacgatc gccacccgct
    30481 ccggcatcag cccgcgcacc gcggcggcga ccaggtgctg gatcgccgcg atctgggtct
    30541 ggctcaggcc gctcgcgccc tgcagccgca ggaacaccga cgcgctcggc tgttcgtgct
    30601 cgcgctggaa cagctccctt tgcggcagca ccaggtggac ccgcgccgcg ccgaccctcg
    30661 acagcaagcc gatcgtgcgc gcgagctccc cttccagggc ccgcaccaga ttgacgttct
    30721 ggacgaaccg cgtggcgccc aggctgtcgg cctcgtcgaa cagctcgtag ccgatcgatc
    30781 cgcccgacgg caggccctgc tcggcgagcc gcagcctgag cctcagcacc tggtcggagg
    30841 gaaccaggat ccggctgccg tcgccaccga tccggtacgg cacggccatc gcttcgagct
    30901 ggttggcgat cgcgcccgaa tcgcgcaccg gcagttcgcc gtagagcagg gtgaagtccg
    30961 gcgtcgtcag ccgggtgaac acgaacatga agaacgcgac caggcccgcg ccgacgccgg
    31021 ccagcaagat caggcgcacg gcgccgaggc tgcgcagcgt ctggacaagt ccgttcactc
    31081 ggtcaccccg tcattcgcgg cgcgcggcac ggacgatccg cgccggccac gccggcccgc
    31141 attcgccacg caccgcgggc aatttgtgca ggccttgatg aacaactcgt aaatggcgga
    31201 atccaaaagc gaagcgcccc ggccgtagtg gcccggggcg ccccatgacg acaccgctgc
    31261 cggcgccgct taccggcgca ggcggatcag ctcgtcgagc atctcgtcgg cggtggtgat
    31321 cacccgcgtg ttggccgagt aggcgcgctg ggtgatgatc agctcggtga actcgcgggc
    31381 gatatcgacg ttcgaggact cgagcgccga cggcacgaag ttgccggcgc cgcccagacc
    31441 cgcctcgcgc aggttgaagt tgcccgaccg attggtctcg gagtaggcgt tgccttcctc
    31501 ggtgcgcagc gacgtcgggt cggcgaaggt cgatatcggc agcttgtagg ccgaccgggt
    31561 ctggccattg gtgaacgatg ccaccacgaa accgtcctcg tcgatgctga caccgttcaa
    31621 gaggccgacc gaggcgccgt tctggttcag gaacgagacg ttgtagttcg agaagaactg
    31681 gctgaccccg tcggcgaggt cgttggtgcc ccagtcgatg tcgatggtgc tggcgctggc
    31741 gccgttggtc caggtgatgc tggtcggggt ttccaatgcg ctggtgatcg ggtccacggt
    31801 gccgatcgtg gtgccggcgt tgtcggcgtc cagcgttgcg tcgccgttga acacgatgtt
    31861 gcccgaggcg agcgcggtcg cgcccgcggt gacctcggcc ggaaccgcgg cgtacacctc
    31921 gaccgcccag gtattgatgc cggtcttgag gtaggcgacg gtcagctcgt gcgatgcgcc
    31981 aagcgaatcg aagaccacga agttgcggaa gaagtgcggc gtcaccgtgc cggcggccat
    32041 gtcgttgacg gcgtaggcac cgccaaagat tgtttggccc gcgtccaggt tggtgccgat
    32101 gccgaccgtg ctcgtcgccg aggcggtgcc gctgaactgg ccgatgttga ccgtggtcag
    32161 gctcgacagg tcggccgagg ccgccggcag gttgccgttc gcgtcgagtg cccagccctg
    32221 caggtaatag ccgctcgagt tgcgcaggtt gccgagcgaa tcgggggtga aggacccggc
    32281 gcgggtatac cgcgccgcgc cgctgccgtc ggtctcggtg ttgaccacca tgaacccgtt
    32341 gccgctgatc gcgacgtcgg tatcggagtt ggtggtcgag atctggccct gctggttgat
    32401 ggtgtagaac ggcgtcgagc gcacgccggc gggcgagtgc gtcgcgagcg acccggagtt
    32461 ggtcaccagg gtcgcaaacc gcgccgtgac ccgcttgtag ccgatggtgt tcaggttggc
    32521 gatgttgtcc gagatcatcg ccatcgactg ggtctgggcg ttgagcccgc tgaccccggc
    32581 aaacaaggcg ccgtagagac tcatcgtcgt actccttgat agtcgagacg gaccgggcgg
    32641 gccgcgccgc gtcaggtctg ctcctgttgt ccttgctggt ccgaaagcgg gccgaggatc
    32701 gccagcacct cgccgagcgg cacgccgctc ttgccgacga acagcaccgg cccatcgccc
    32761 gcggtctcga tgccggtcac cgtgccgacc gtggtcatcg tcgcggtcat gctgttgcct
    32821 tcgggatcgg tggcggtcac cgccagcgtg tattggccct cgggcatcgg gttgccggcg
    32881 tcgtcgaggc cgctccagac gaactggtgt gcgcccgcct cggttgcgcc ggtggtgctg
    32941 taaatcgccc tgccggcctc gttggtgacc agcagcgtca cctggttcgc ggcgccgggc
    33001 agggcgtagt tccactcggc gcggccgtcg gccagcgccg tggtgttgcc gttggcctcg
    33061 atgcgctggc ccagatagcc gatcagcccc gccgcctggt tggcgcgctg aagggcgatc
    33121 agctgctcga ggttggcgtt ggtcgcgatc gcctgctcga cgccggcgaa gcgcacgatc
    33181 tggctcgtga actcgttggt gtccatcggc gacaacgggt cctggttgcg gagctgggtg
    33241 atcagcaaca tcaggaaatt gtcgaaattt tcggccaggt cgcggccggc gatcacggac
    33301 gcctgctgcg cctgtttggt ggtgacggcg gaaacttcca tggctcggac ctctcgctcg
    33361 gctcacacgc ggatgtcgat tccggcggcg cccgggccga tcaggagcgc gcccggaccg
    33421 gcgccgatct cggcctccgc catcgcgcgc aatggatcgg gcgggccggc cgcgatgtcg
    33481 cccgcgccgc ggtcgtcatg cccgtccccg aagtcccgat ccaacaggtc gaacgccagg
    33541 ctgtcggaat cgagatcgaa accggcgtct tgcagggccc gctcgagccc gcgcgcatcg
    33601 cgcatcatca gctccaatgt ctcggcgcgc tggaccagca catgggccga cagccggccg
    33661 ccgtcgccaa gctcgagccg gacctcgacg cggccgagct ccttcgggtg aagctggatg
    33721 cggacgtggc tgagctcgtc ctcgatcgcc tggtggatgt gcagcacgat ctggtcgatg
    33781 gccggcatcg gcggcggcgc ggcgcgggca acggctgcgg cggcgctcgc cgccag
//
LOCUS       DASWHU010000255         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_509891, whole genome shotgun sequence.
ACCESSION   DASWHU010000255 DASWHU010000000
VERSION     DASWHU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509891"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGB88_09465"
     CDS             complement(<1..270)
                     /locus_tag="VGB88_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9647715.1"
                     /translation="MKIAKIEDLHCDAGWRTFSFLKVTTDDGLAGWSEYNESYGSAGL
                     TAVIRGLAELVVGEDARATERITAKLYAKTRHAAGGMIQQAIAALE"
     gene            complement(536..1330)
                     /locus_tag="VGB88_09470"
     CDS             complement(536..1330)
                     /locus_tag="VGB88_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9647716.1"
                     /translation="MTALDTLAPTSPRTPPGIGAAEWQARVRLAACYRIADHLGWTET
                     IYGHITLRVPGPDKHFLINPWGLRYDEVRASNLVKVGLDGSIVGDSDWGINPAGFVIH
                     SAIHGARADVACVVHVHTIAGMAVAAQARGLLPVSMPATGFWGRIAYHDYEGPSLELD
                     ERARLVRDLGDKKVMILRNHALLTCGATLEEAFILMFRLQRACEVQIAAQGGGAPLVE
                     PSEEVCEESARLTDRFLSGEGAAPVGKLEFDSYVRLIEARDPSFRS"
     gene            1582..3273
                     /locus_tag="VGB88_09475"
     CDS             1582..3273
                     /locus_tag="VGB88_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9647717.1"
                     /translation="MVAQRKPSRRWWAPIALCVVGVSIAAAAAPTVSDWLMAGFDLHN
                     TRHQPNETILGPDTVGGLKIKWVARLGGSVSATPAVDATSVYVPDWRTWWLSPHGGKL
                     YRIDRGTGRIIWARRVSDYTGIAGDFSRTTPIVSGDRLILGNQAGRLLWPGVPGTVAQ
                     VVSVDKATGNLVWATKVDDNPASIVTSSPVAHDGVVYVGVSSQEERHAAFIPGYVCCT
                     FRGSVVALDEATGAILWKTYTVPTDADGEPLPGYSGNAVWGSSPVVDVERNRLYVGTG
                     NNYRLPKPVSDCIAGAGDDEDQIRACDPAVGIDDDGDNYFDSVLALDLDDGSIKWGRR
                     FRPYDEWVASCLLPVLLNPNNCSKRPGVDHDFAQGPALLDGILGIGQKSGDYWGLDPE
                     TGAVVWHAKVGPGGLYGGMEWGSATDGDRIYVGVANFPTLAPWTLLDGRTVWTGFWAA
                     LDAPTGEVLWQTPSLLIGGAQGGITVANGVIYAGSHGPTVFAPHRWLFRGPTMLALDA
                     VTGEVLWDFETVGSVIGAPAVVDGVVYWGAGFESLGLVLGEQPDNKLYAFGLADD"
     gene            complement(3340..>3935)
                     /locus_tag="VGB88_09480"
     CDS             complement(3340..>3935)
                     /locus_tag="VGB88_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX9647718.1"
                     /translation="AEADAAWLRAWYGLPPPAEAVAFRAGAAWVISLDDALYLGARRV
                     ADDAGPLVGAAALDGMIVAASAQALFLLTPEGEMVERLGAALLPGPLDALGAGGGGQV
                     VVVSGSAAYAADSSLARWADAPLPAAYVAPATPPAALLADIRARHGGPGVSWERVILD
                     LHSGRLLGRLGPTIVDAAALALGLLAFSGLYNAWRRS"
BASE COUNT          570 a         1380 c         1404 g          581 t
ORIGIN      
        1 ctcgagcgcg gcgatcgcct gctggatcat gccgccggcg gcgtggcggg tcttggcgta
       61 gagcttggcc gtgatccgct cggtggcgcg cgcatcctcg cccacgacca gctcggcgag
      121 gccgcggatg accgcggtca gacccgcact cccgtaggac tcgttgtact cggaccaacc
      181 cgcgagcccg tcgtcggtgg tcactttgag gaacgagaag gtgcgccagc cggcgtcgca
      241 gtgcaggtcc tcgatcttgg cgatcttcat ccgcgcgccc tcccttcgtt cgtcggctaa
      301 ccattctgaa cacaagactc gcgccgcacg tcctgtcgtc attcccgcga aagcggcaat
      361 ccagttgttc ggtctctatt ctggaccccc gctttcgagc gtgtgaagca atcgccacac
      421 ggccctctcg tcgtcatgcc cgcgaacgcg ggcatccagt cttttcaata agttgttgga
      481 cccccgcttg cgcggggtga cggtgacaga ggcgcatcga ttgcggtcac gcgcctcagc
      541 ttcgaaacga cgggtcgcgc gcctcgatca gccggacgta ggagtcgaac tcgagcttgc
      601 ccaccggcgc cgcgccctcg cccgacagga accggtcggt gagccgcgcc gactcctcgc
      661 acacctcctc cgaaggctcg accaggggcg cgccgccgcc ttgcgccgcg atctgcacct
      721 cgcaggcgcg ctgcaggcgg aacatgagga tgaatgcctc ctcaagcgtc gccccgcagg
      781 tcagcagcgc gtggttgcgc aggatcatca ccttcttgtc gcccaagtcg cgcaccagcc
      841 gcgcgcgctc gtcaagctcg aggctcggcc cctcgtaatc gtgataggcg atccgccccc
      901 agaagccggt cgccggcatc gacacgggaa gcagcccgcg cgcctgcgcc gccaccgcca
      961 tgccggcgat ggtgtggacg tggaccacgc aggcgacgtc ggcgcgcgcg ccgtggatgg
     1021 cgctgtggat gacgaacccg gccgggttga tgccccagtc gctgtcgccg acgatgctgc
     1081 cgtcgaggcc gaccttgacc aggttggacg cgcgcacctc gtcgtagcgc aagccccacg
     1141 ggttgatcag gaagtgcttg tccggccccg gcacgcgcag cgtgatgtgg ccgtagatgg
     1201 tctcggtcca gccgagatgg tcggcgatcc ggtagcaggc ggcaagccgc acccgcgcct
     1261 gccactccgc cgcgccgatg ccgggcgggg tgcgcggcga ggtcggggcc aaggtgtcga
     1321 gtgcggtcat ggcgcgcctc cgccggctgg ggtcgaggcg gccatgctag cacgccgcgc
     1381 ccgccttgcg ggaggggctc gatcggacac ggccgccggc gccagctcga tcgaaaactc
     1441 tcggttcgcg tctcccgcgc gctgcgtccc ctgtcacgca gcggcccgct gggatataac
     1501 agtgcgggct ttgagcgcgt aaccggctgc gaggtctggc gcaggtcacg tctttcgggg
     1561 cccgggaggg ggcgtcggtt gatggtcgcg caacggaaac cgtcgaggcg gtggtgggcg
     1621 ccgatcgcac tgtgcgtggt cggggtgagc atcgcggccg cggctgcgcc gacggtgagc
     1681 gactggctga tggccgggtt cgatctccac aacacgcgcc accagccgaa cgagacgata
     1741 ctggggcccg acacggtcgg cgggctgaag atcaaatggg tggccaggct cggcggcagc
     1801 gtctcggcga cgcctgcggt cgacgcgacc tcggtctacg tgcccgattg gcggacatgg
     1861 tggctgtcgc cgcacggcgg caagctctac cggatcgacc gcggcaccgg caggatcatc
     1921 tgggcgcgcc gggtcagcga ctacactggg atagccggcg acttctcgcg caccacgccg
     1981 atcgtgagcg gcgaccgcct gatcctcggc aaccaggccg gccggctgct ctggccggga
     2041 gtgccgggga ccgtggcgca ggtggtctcg gtggacaagg cgaccggcaa cctggtctgg
     2101 gcgaccaagg tcgacgacaa cccggcaagc atcgtcacca gctctccggt cgcgcatgac
     2161 ggcgtcgtct atgtcggcgt ctcctcgcaa gaggaacgcc acgccgcctt cattcccggc
     2221 tacgtctgct gcacgttccg gggcagcgtc gtcgcgctcg acgaggcgac cggtgcgatc
     2281 ctgtggaaga cctataccgt gccgacggac gccgacggcg agccgctccc gggctacagc
     2341 ggcaatgcgg tctggggcag ctcgccggtg gtcgatgtcg aacggaaccg cctctacgtc
     2401 ggcaccggca acaattaccg gctgccgaag ccggtttccg actgcatcgc cggcgccggc
     2461 gacgacgagg accagatccg agcgtgcgat cccgccgtcg gcatcgacga cgacggcgac
     2521 aattacttcg attccgtgct cgcgctcgat ctggacgacg gctcgatcaa atggggccgg
     2581 cgcttccggc cgtacgacga gtgggtcgcc tcgtgcctgt tgccggtcct cttgaacccg
     2641 aacaattgct cgaagcgccc gggcgtcgac cacgacttcg cgcaagggcc ggcgctgctc
     2701 gacggcatcc tcggcatcgg ccagaaaagc ggcgactact ggggactcga cccggagacc
     2761 ggcgccgtcg tctggcacgc caaggtcgga ccgggcgggc tttacggcgg catggagtgg
     2821 ggatcggcga ccgacggcga ccgcatctat gtcggggtcg ccaactttcc gacccttgcg
     2881 ccctggacgc tgttggacgg ccgcacggtg tggacgggat tctgggccgc gctcgacgca
     2941 ccgaccggcg aggtgctgtg gcaaacgccg agcctcctta tcggcggcgc gcagggcggg
     3001 atcaccgtcg ccaacggcgt catttacgcg ggctcccacg ggccgacagt cttcgcgccg
     3061 caccgatggc tgttccgcgg cccgacgatg ctcgcgctcg atgccgtgac cggcgaggtc
     3121 ttgtgggact tcgagaccgt cggctccgtg atcggcgcgc cggccgtcgt cgacggtgtc
     3181 gtctattggg gcgcgggctt cgagagcctg ggcctggtgc ttggcgagca gcccgacaac
     3241 aagctctacg cgttcgggct ggccgacgac tgagagggcg attgatccgc gggcgctcgg
     3301 cgcgccctgg gtcggccgcc gatgcggcgg cgcgcgcgct cagctccgcc gccaggcgtt
     3361 gtacaggccg ctgaaggcta gcaggccgag cgccagcgcc gcggcatcga cgatggtcgg
     3421 cccgaggcgg ccgagcaggc ggccgctgtg caggtcgagg atcactcgct cccacgacac
     3481 gccggggccg ccgtggcgtg cgcggatgtc ggcgagcagc gccgccggcg gcgtcgcggg
     3541 cgccacgtaa gcggccggca agggcgcgtc cgcccagcgt gccagcgagg agtcggcggc
     3601 gtaggccgcg ctgccgctga ccaccaccac ctgcccgccg ccgccggcgc cgagcgcatc
     3661 gagcgggccg ggcagcaggg ccgcgcccag ccgctcgacc atctcgccct cgggcgtcag
     3721 caggaacagc gcttgggcgc tggcggcgac gatcatgccg tcgagcgccg cggcgccgac
     3781 cagcggcccg gcatcgtcgg cgacgcggcg cgcgccgaga tagagcgcgt catcgagcga
     3841 gatcacccat gcggcgccgg cgcggaacgc gaccgcctcg gccggcggcg gcaggccgta
     3901 ccacgccctc agccaggcgg cgtcggcctc agcgc
//
LOCUS       DASWHU010000256         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3442456, whole genome shotgun sequence.
ACCESSION   DASWHU010000256 DASWHU010000000
VERSION     DASWHU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3442456"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VGB88_09485"
     CDS             complement(<1..656)
                     /locus_tag="VGB88_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX9647719.1"
                     /translation="MNFRSDNEAPVAPEIMAALARANHGAAHSYGADQPTARLQAVFA
                     ELFETELAVYPVATGTAANALCLAELTPPYGAVYCHAQSHVNVDECGAPEFYTGGAKL
                     VGIDGAHGRVEADRLAALLAATGAHGEHESAPATLSLTEATEAGTVYTPDSVAGLSAV
                     ARRHGMAVHMDGARIANAVSRLGCTPAELTWRAGIDMLSFGATKNGALAAEAVVDFRP
                     A"
     gene            complement(679..1149)
                     /locus_tag="VGB88_09490"
     CDS             complement(679..1149)
                     /locus_tag="VGB88_09490"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX9647720.1"
                     /translation="MTVVIAPADLRAIVDAAEAAYPEECCGLLVGRMRRGRVLVREAH
                     ASRNLAEDPRRAFEVDPRVQFALLRRLRGGPDDVIGVYHSHPDLGAEPSATDLARAWE
                     PGLIWLITSVLGGQAVLTTAHRLSADRRRFRRLGLATGEVRAYASRTRPARTGG"
     gene            complement(1146..1433)
                     /locus_tag="VGB88_09495"
     CDS             complement(1146..1433)
                     /locus_tag="VGB88_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647721.1"
                     /translation="MTIQLLKVLVVAMGALILAGLAVIGVTIANRLGAMSQPRAGDIE
                     VAVPAGSTVVETTAAGDRLVLRLDTPAGPRLVVVRLSDGTVEATIVLVPAP"
     gene            1454..2455
                     /locus_tag="VGB88_09500"
     CDS             1454..2455
                     /locus_tag="VGB88_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9647722.1"
                     /translation="MTTTARDPARPRPVAHELAVPRQAAGERLDRFLAARIESLSRSR
                     LKALIQAGAVAVDGAPENDPARKLRPGQAVRVAVPAPADAAPRAQAIALDVVHEDAHL
                     IVIDKPAGLVVHPAPGNPDRTLVNALLAHCGASLAGVGGVRRPGIVHRLDKGTSGLMV
                     VAKSDAALAGLADQFAHRTILRAYRAVVWGVPAPAAGVIEGAIGRNPRNRKKMAVVER
                     GGKPARTRYRVERALGEGLASVVVCRLETGRTHQIRVHLAHLGHGVIGDPVYGRGPAR
                     LKRAAPAAARSFPHQALHAFLLGFVHPLTRQQLRFEVGLPEDIEKLIDAFGKTGQTP"
     gene            2588..>3360
                     /locus_tag="VGB88_09505"
     CDS             2588..>3360
                     /locus_tag="VGB88_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX9647723.1"
                     /translation="MVAMNLPSVSSEGGLARYLEEIRRFPMLTFDEEYMLARRWREHG
                     DIVAAHRLVTSHLRLVAKIAMGYRGYGLPVGELISEGNVGMMQAVRRFDPERGFRLAT
                     YAMWWIRAAIQEYILHSWSLVKMGTTAAQKKLFFNLRKLKGQLQAIDEGDLPQETVTE
                     IARRLDVPEAEVVNMNRRMASPDHSLNAPLRVDGDGEWIDWLVEQEDDQESRLAESEE
                     LGQRRAMLEVAMGRLNDRERHIFTERRLREDPKTLEELS"
BASE COUNT          479 a         1207 c         1220 g          454 t
ORIGIN      
        1 gcggggcgga agtcgaccac cgcctcggcg gcgagcgcgc cgttcttggt cgcgccgaac
       61 gacagcatgt cgattccggc gcgccaggtc agctcggcgg gggtgcagcc gagccggctc
      121 accgcattgg cgatccgcgc cccgtccatg tgcacggcca tgccgtgacg ccgggcgacg
      181 gcactcaagc cggcgaccga atcgggggtg tacacggtgc cggcctcggt ggcctcggtc
      241 aggctgaggg tcgccggcgc cgattcatgc tcgccatggg cgccggtcgc ggccagcagc
      301 gcggccagcc ggtccgcctc gacgcggccg tgggcgccgt cgatgccgac cagcttggcg
      361 ccgccggtgt agaactcggg cgcgccgcac tcgtcgacgt tgacgtgcga ttgggcgtgg
      421 caatagaccg cgccataggg cggcgtcagc tcggccaggc acagggcgtt ggcggcggtg
      481 ccggtggcga ccgggtagac cgcgagttcg gtctcgaaca gctcggcgaa gaccgcctgc
      541 aggcgcgcgg tcggctggtc ggcgccatag gaatgggcgg cgccgtggtt ggcgcgcgcc
      601 agcgcggcca tgatctcggg cgcgaccggc gcctcgttgt cgctgcggaa gttcatgcgc
      661 gctctctgcc ggttctggtc agccgccggt ccgggcgggt cgggtgcggc tggcataagc
      721 gcgcacctcg ccggtggcaa gcccgagcct gcggaaccgg cggcggtcag cgctcagccg
      781 gtgggcggtg gtgagcaccg cctggccgcc gagcaccgag gtgatcagcc agatcagccc
      841 cggctcccag gcgcgcgcga ggtcggtggc ggacggctcg gcgccgagat ccggatgcga
      901 gtggtagacg ccgatcacgt cgtcgggccc gccgcgcagg cgcctcagca gcgcgaactg
      961 cacccgcggg tcgacctcga acgcgcgccg cggatcctcg gccaggttgc ggctggcatg
     1021 ggcctcgcgc accagcaccc ggccgcggcg catccgcccc accagcagcc cgcagcactc
     1081 ctcgggatag gcggcctcgg cggcatcgac gatggcccgc aggtcggccg gcgcgatcac
     1141 cacggtcatg gcgccggcac cagcacgatg gtcgcctcga ccgtgccgtc gctgaggcgt
     1201 accaccacca ggcgcgggcc cgcgggcgtg tccaggcgca gcaccagccg gtcgccggcg
     1261 gcggtggtct cgaccacggt gctgccggcg gggaccgcga cctcgatgtc gccggcgcgc
     1321 ggctgcgaca tcgcgcccag gcggttggcg atggtcaccc cgatcaccgc caggccggct
     1381 aggatcaggg cgcccatcgc caccaccagc accttgagca gctgtatagt catgcccgag
     1441 cgcaaaagtc ccgatgacca cgaccgcccg cgatcccgcc cggccgcgcc cggtggccca
     1501 tgagctggcc gtgccccggc aggcggcggg ggagcggctc gaccggttcc tcgccgcgcg
     1561 catcgagtcg ctgtcgcgca gccggctgaa ggcgctgatc caggccggcg cggtggcggt
     1621 cgacggcgcc ccggagaacg acccggcgcg caagctgcgc cccggccagg cggtgcgcgt
     1681 ggcggtgccc gcgccggcgg acgccgcgcc gcgcgcgcaa gccatcgcgc tcgacgtggt
     1741 ccatgaggac gcgcacctga tcgtgatcga caagccggcc gggctggtcg tccaccccgc
     1801 cccgggcaat cccgaccgca ccctggtcaa cgcgctgctc gcccattgcg gcgcgagcct
     1861 cgccggggtc ggcggcgtgc gccggccggg catcgtccat cgcctcgaca agggcaccag
     1921 cggcctgatg gtggtggcca agtccgacgc cgcgctcgcc gggctcgccg accagttcgc
     1981 ccaccgcacc atcctgcgcg cctatcgggc ggtggtctgg ggggtgccgg cgccggctgc
     2041 gggcgtgatc gagggcgcga tcggccgcaa cccgcgcaac cgcaagaaga tggcggtggt
     2101 cgagcggggc ggcaagccgg cgcgcacccg ctaccgcgtc gagcgcgccc tcggcgaggg
     2161 cctcgccagc gtggtggtgt gccggctcga gaccgggcgc acgcaccaga ttcgggtcca
     2221 tctcgcccat ctcggccacg gcgtcatcgg cgatccggtc tacggccgcg gcccggcccg
     2281 gctcaagcgc gcggcgccgg cggcggcgcg ctcgttcccc catcaggcct tgcacgcttt
     2341 cctgctcggc ttcgtccatc cgctgacccg ccaacagctg cgattcgagg tcggcctgcc
     2401 cgaagatatc gaaaagttga tcgacgcctt cgggaaaacg gggcaaacgc cttaatttcg
     2461 ccccctttcg tccaaaaaat cgacccaact taaggcttta gtgcgcgtct gacgccgccg
     2521 aatgccgttt ggcagtgggc ggcatcgagt gctaaagtat caccaaccgg ccggaggcct
     2581 ctcaactatg gttgccatga atcttccctc ggtctcgtcg gagggcgggc tggcgcggta
     2641 tctcgaggag atccgccgct tccccatgct caccttcgac gaggagtaca tgctcgcccg
     2701 gcgctggcgc gagcacggcg acatcgtggc cgcccaccgg ctggtgacca gccatttgcg
     2761 cctggtggcc aagatcgcca tgggctatcg cggctatggc ctgccggtcg gcgagctgat
     2821 ctcggaaggc aatgtcggca tgatgcaggc ggtcaggcgc ttcgacccgg agcgcggctt
     2881 ccggctcgcc acctacgcca tgtggtggat ccgggcggcg atccaggagt acatcctgca
     2941 ctcctggtcg ctggtcaaga tgggcaccac cgccgcccag aagaagctgt tcttcaacct
     3001 gcgcaagctc aagggccagc tgcaggcgat cgacgagggc gacctgcccc aggagaccgt
     3061 gaccgagatc gcgcgccgcc tcgatgtgcc cgaggccgag gtggtgaaca tgaaccgccg
     3121 catggccagc cccgaccatt cgctgaatgc gccgctcagg gtcgacggcg acggcgagtg
     3181 gatcgactgg ctggtcgagc aggaggacga ccaggagtcg cgcctcgccg agagcgagga
     3241 gctcggccag cgccgggcca tgctcgaggt ggcgatgggc cggctgaacg accgcgagcg
     3301 ccacatcttc accgagcggc gcctgcgcga ggacccgaag acgctcgagg agctgagcca
//
LOCUS       DASWHU010000257         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_126515, whole genome shotgun sequence.
ACCESSION   DASWHU010000257 DASWHU010000000
VERSION     DASWHU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126515"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VGB88_09510"
     CDS             complement(<1..1193)
                     /locus_tag="VGB88_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX9647724.1"
                     /translation="MRDLHFPGRSAVHAFNAMAATSHPAATMVALETLRAGGNAMDAA
                     ISAAAVLAVVEPHSTGIGGDVFALYMLKGQGRVIALNGSGRAPQAATAEALRARGLSE
                     IGFRSPHAVTVPGAVAAWEKLSDDHGRLGLDRLLEPAIRFARDGYPVHEVVAHVWARE
                     YDKLAADPNARAVFLAKQRPLTAGEIHRQPALADTLEAIAEGGRDAFYTGAIADDMVS
                     YLRSVGGLHTMDDFAAARADYVEPISTDYRGLTVWECPPNGQGIIALMMLNILSGFEL
                     GKLDPLGAERLHLEAEATRLAYRDRLTLLADPDHAEVPVKKLLSAGYAEALRALICPD
                     RALRDMPPPGEVAHRDTVYLTVVDRERNAVSFINSLFHSYGTGLMSPKAGVMFHNRGS
                     GFSLDP"
     gene            1334..2242
                     /locus_tag="VGB88_09515"
     CDS             1334..2242
                     /locus_tag="VGB88_09515"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647725.1"
                     /translation="MTVTVALLGTGLMGAPMARNIAKAGFGLSVWNRTRAKAEALADA
                     GAAVADSAAEAVRAADLVITMLETGPVVRQVLFAGGVADAMRTGALVADMSSIPPRMA
                     REHAERLAGLGIGHLDAPVSGGVRGAEKATLAIMVGGDETTFRRAAPVLEAMGRATLV
                     GPPGAGQLAKLTNQTIVAVTIAAVAEGLLLAAAGGADPKAVQAALAGGFADSIILQNH
                     GSNMIARRFLPGGRAEVQLKDLDTIHDTAAELGLTLPAAECVRGLFRDLVAAGDGAVD
                     HCGVLLQEERINAPARLGAGPDIRPD"
     gene            2277..>2618
                     /locus_tag="VGB88_09520"
     CDS             2277..>2618
                     /locus_tag="VGB88_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647726.1"
                     /translation="MRAVLLVAALAVTALAGAARSGDAAGTISAAVDGEARGWVVAAG
                     DSGWRPADGMIALTIVGHAEPAAGTIMLELTLTELAGDAARLFGTEAMLVAQDITYVP
                     GSGVPPYASAAG"
BASE COUNT          340 a          952 c          991 g          335 t
ORIGIN      
        1 ggatcgaggc tgaagcccga gccgcggttg tggaacatca cgccggcctt cgggctcatc
       61 agcccggtgc cgtaggagtg gaacagcgag ttgatgaacg agaccgcgtt gcgctcgcga
      121 tcgaccacgg tgagatagac cgtgtcgcgg tgcgcgacct cgcccggcgg cggcatgtcg
      181 cggagcgcgc ggtcggggca gatgagcgcg cgcaaggcct cggcgtagcc cgccgacaac
      241 agtttcttga ccggcacctc ggcgtggtcg gggtcggcga gcaaggtcag ccggtcgcgg
      301 taggcgagcc gggtcgcctc ggcctcgagg tgcaggcgct cggcccccag cgggtcgagc
      361 ttgccgagct cgaagcccga gaggatgttg agcatcatca gggcgatgat gccctggccg
      421 ttgggcgggc actcccacac cgtgaggccg cggtagtcgg tcgagatcgg ctcgacgtag
      481 tcggcgcgcg cggcggcgaa gtcgtccatc gtgtgcagcc cgccgacgct ccgcaggtag
      541 ctgaccatgt cgtctgcgat cgcgccggtg tagaaggcgt cgcgcccgcc ttcggcgatc
      601 gcctcaaggg tgtcggcgag cgccggctgg cggtggatct cgcccgccgt cagcgggcgc
      661 tgcttggcca ggaacacggc gcgcgcgttg gggtcggcgg cgagcttgtc gtactcgcgc
      721 gcccacacat gcgccaccac ctcgtgcacc gggtagccgt cgcgggcgaa cctgatcgcg
      781 ggctccagca ggcggtcgag accgagccgg ccgtggtcgt cggacagctt ctcccaggcg
      841 gcgacagctc ccggcacggt caccgcgtgc ggcgagcgga agccgatctc gcttaagcct
      901 cgcgcgcgca gcgcctccgc cgtcgccgcc tgcggcgcgc gccccgagcc gttgagcgcg
      961 atcacccgcc cctggccctt gagcatgtag agcgcgaaca cgtcgccgcc gatgccggtc
     1021 gagtgcggct cgaccacggc gaggaccgcc gccgcggaaa tcgccgcgtc catcgcgttg
     1081 ccgccggcgc gcaacgtctc cagcgcgacc atggtcgcgg ccgggtgcga ggtcgcggcc
     1141 atggcgttga acgcgtgcac ggccgagcgg ccgggaaagt ggaggtcgcg catcagatca
     1201 ccccagtagc gtggtcggcg ggtgcggccc gccttctagt agcggcggcg cgcccctgcg
     1261 gcaagccccg ctttggcgtt caatcgacgg cggccggccc tatagtgcgc gggcacaggc
     1321 cagggaggga gcgatgaccg tcacggtggc actgctgggg accgggctga tgggcgcgcc
     1381 gatggcgcgc aacatcgcca aggcgggctt cgggcttagc gtctggaacc gcacccgcgc
     1441 caaggccgaa gcgctggccg acgccggggc ggcggtcgcc gacagcgccg ccgaggcggt
     1501 gcgcgcggcc gacctggtga tcaccatgct cgagaccggg ccggtggtgc gccaggtgct
     1561 gttcgcgggc ggcgtcgccg acgcgatgcg caccggcgcg ctggtcgccg acatgagctc
     1621 gatcccgccg cgcatggcgc gcgagcacgc cgagcgcctg gccggcctcg gcatcggcca
     1681 tctcgacgcg ccggtctcgg gcggcgtgcg cggggccgag aaggcgacgc tcgcgatcat
     1741 ggtcggcggc gacgagacga ccttccggcg cgcggcgccg gtgctcgagg cgatgggccg
     1801 cgcgaccctg gtcggcccgc ccggcgccgg ccagctcgcc aagctgacca accagaccat
     1861 cgtcgcggtg acgattgccg cggtcgccga gggcctgctg ctggcggccg cgggcggcgc
     1921 cgaccccaag gcggtgcagg ccgcgctcgc cggcggcttc gccgactcga tcatcctcca
     1981 gaaccacggc agcaacatga tcgcgcgccg gttcctgccc ggcggccggg ccgaggtgca
     2041 gctcaaggac ctcgacacca tccacgacac cgccgccgag ctcgggctga ccctgcccgc
     2101 ggccgaatgc gtgcgcgggc tgttccgcga cctggtcgcg gccggcgacg gcgcggtcga
     2161 ccactgcggg gtgctgttgc aggaggagcg gatcaacgcc ccggcgcgcc tcggcgccgg
     2221 ccccgacatc cgccccgatt agcggccccg gccgtgcggc ggcggcgccg gtcgccatga
     2281 gggcagtcct cctcgtcgcc gccctcgccg tgacggctct cgcgggcgcg gcgaggtccg
     2341 gtgacgccgc cggcactatc tcggccgccg tagacggcga ggcgcgcggc tgggtggtcg
     2401 cggccggcga cagcggctgg cggcccgccg acggcatgat cgcgctgacc atcgtcggcc
     2461 acgccgagcc tgccgccggc acgatcatgc tcgagctgac cctgaccgag ctcgcgggcg
     2521 acgccgcacg cctgttcggc accgaggcga tgctggtcgc ccaggacatc acctacgtcc
     2581 cgggcagcgg cgtcccgccc tacgccagcg ccgccggc
//
LOCUS       DASWHU010000258         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2002122, whole genome shotgun sequence.
ACCESSION   DASWHU010000258 DASWHU010000000
VERSION     DASWHU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002122"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VGB88_09525"
     CDS             <1..288
                     /locus_tag="VGB88_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9647727.1"
                     /translation="DAGGRTALIWAAFHDHAHMLEFLLARGADVNAQDAHGRTALIWA
                     ATAGRAEAARALLAAGADPGHRDRDGLGARDWAERGGHAGLAGLLAGAAAD"
     gene            309..1262
                     /locus_tag="VGB88_09530"
     CDS             309..1262
                     /locus_tag="VGB88_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9647728.1"
                     /translation="MSGPESWIALAAAIALDAYLGASLGAGAGRWHPKALIGRTLAWS
                     EARLNRPHRSARDRRLRGALALAAAVIAAVVAGAALAGLARAAPIVWVPLALLACALI
                     DLRTEHEDARALAAALASGDGAAAHARLARLSARDPAALDAYGVARAGIEGVLARFAS
                     GWLGPVAAWAALGPAGLLGYWVVAVAGERLGGRAQRLADFGAASRAAARGLATVTDRL
                     AGLVLVAGAVPSAGADAAAAWRTVRGQRAAWTVAAAAGALGLALGGPRRYPARTVQAP
                     WIGAGRARAGADDILGALALVRAGALVSFALVVLAAAASAA"
     gene            complement(1271..1969)
                     /locus_tag="VGB88_09535"
     CDS             complement(1271..1969)
                     /locus_tag="VGB88_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX9647729.1"
                     /translation="MRTHYDRARAPAAALLICASLMALPLSPAAANDAQKLVDDATSM
                     VRAMLVDPDWQDFNSAYKRAKAVVLVPDFLKAGFVVGGAGGQCLMVARSGPDGAWSAP
                     SFCLIGEASFGLQIGAQKSEVLMLVMTDGALERLASGNAKLGGNAGLSVGLIGAGVQG
                     ATTLNLGADIYAFARSQGLYGGVALDGGWIGPDTDYNQAYYGRAVSARNILIDRRVTN
                     PATNGLLAALDSAK"
     gene            complement(2101..>2421)
                     /locus_tag="VGB88_09540"
     CDS             complement(2101..>2421)
                     /locus_tag="VGB88_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647730.1"
                     /translation="RWRTGMTDRSPSALEADSLQERIAFSGRVWQFWALAGVLAAGVG
                     IAVWQDVPTIYIVAGIAAWIVFSLHLSFTAVLHELMELNDQIAGRKDEFRRLVEANQE
                     SQVS"
BASE COUNT          293 a          896 c          907 g          325 t
ORIGIN      
        1 gacgcaggcg ggcggacggc gttgatctgg gcagcgtttc atgaccatgc gcacatgctc
       61 gaatttctgc tcgctcgcgg cgccgacgtc aatgcacagg acgcgcatgg ccgcaccgcg
      121 ctgatctggg ccgcgaccgc cgggcgggcc gaggccgcgc gggcgctgct cgcggccggc
      181 gcagaccccg gccatcgcga ccgggacggg ctcggcgcgc gcgactgggc ggagcggggc
      241 ggccacgccg ggctcgccgg cctgctcgcc ggcgccgccg cggactagcc ggcgccgcga
      301 ggcgaccggt gtccgggccc gagtcctgga tcgcgctggc ggcggcgatc gcgctcgatg
      361 cctatctcgg cgcgtcgctg ggcgccggcg ccggccgctg gcaccccaag gcgctgatcg
      421 ggcgcacgct cgcctggtcg gaggcgcgcc tcaaccgccc gcaccggagc gcccgcgacc
      481 gccggttgcg cggcgcgctg gcgctcgcgg cagcggtgat cgccgccgtc gtcgccgggg
      541 cggcgctggc cgggctggcg cgggcggcac cgatcgtgtg ggtgccgctc gcgctgctcg
      601 cctgcgcgct catcgacctg cgcaccgagc acgaggatgc gcgcgcactg gccgcggcgc
      661 tggcgagcgg cgacggcgcc gccgcgcacg cccggctcgc ccgcctcagc gcccgcgacc
      721 cggccgcgct cgatgcctac ggcgtcgccc gcgccggcat cgagggcgtg ctcgcgcgct
      781 tcgcgtcagg ctggctcggc ccggtggcgg cgtgggcggc gctcggcccg gccgggctgc
      841 tcggctactg ggtggtggcg gtcgcgggcg agcggctcgg cgggcgggcg cagcggctgg
      901 ccgacttcgg cgccgcgtcg cgggccgcag cgcgcggcct cgccaccgtc accgaccggc
      961 tcgccggcct ggtgctggtc gcgggcgcgg tgccgtctgc gggcgccgat gccgccgccg
     1021 cctggcgcac cgtgcgcggg cagcgcgcgg cctggaccgt cgccgccgcc gccggcgcgc
     1081 tcggcctcgc cctcggcggc ccgcgccgct accccgcgcg cacggtccag gcgccgtgga
     1141 tcggcgccgg ccgggcgcgg gcgggcgccg acgatatcct cggcgcgctc gccctggtgc
     1201 gcgcgggcgc gctggtcagc ttcgcgctgg tggtgctcgc cgccgccgcc agcgccgcct
     1261 gagccgcgcc ctacttggcc gagtcgagcg cggcgagcag gccgttggtc gccgggttgg
     1321 tcacccgccg gtcgatcagg atgttgcgcg cgctcaccgc ccggccgtaa taggcctggt
     1381 tgtagtcggt gtcggggccg atccagccgc cgtcgagggc gaccccgcca tagaggccct
     1441 ggctgcgcgc gaacgcatag atgtcggcac cgaggttgag cgtggtcgcg ccctggacgc
     1501 cggcgccgat caggccgacc gaaagcccgg cgttgccgcc gagcttggcg ttgcccgagg
     1561 cgagccgctc gagcgcgccg tcggtcatca ccagcatcag cacctcggac ttctgggcgc
     1621 cgatctgaag gccgaagctc gcctcgccga tcaggcagaa cgacggcgcc gaccaggcgc
     1681 cgtccgggcc gctgcgcgcg accatcaggc actggccgcc ggcgccgccg accacgaacc
     1741 cggccttgag gaagtcgggc accaggacca ccgccttggc gcgcttgtag gcgctgttga
     1801 agtcctgcca atcgggatcg accagcatcg cgcggaccat cgaggtcgcg tcgtcgacca
     1861 gcttctgggc atcgttggcg gccgccgggc tgagcggcag cgccatcagg gaggcgcaga
     1921 tcagcagcgc cgcggcaggc gcgcgggcgc ggtcataatg cgtgcgcatg atatctccgg
     1981 gagttgtctt ggggagggga ggtgtgcagg cacgggcctg agtctaacag acggcgagtc
     2041 gtcaacgcga gcccgcgatg cggttagtgc tggctgaatt aggacaccca ggctagggga
     2101 ttaggacact tgggattcct ggttggcctc gaccagcctc ctaaattcat ccttgcggcc
     2161 cgcgatctga tcgttcagct ccatcaactc gtgcaggacc gcagtgaagc tgagatgcag
     2221 agagaacacg atccacgcgg caatccctgc aacgatatag atggtcggaa cgtcctgcca
     2281 aaccgcgata cccacgccgg cagccaatac ccccgccagc gcccagaact gccacactcg
     2341 cccactgaat gcgatccgct cttgcaggct gtccgcctct agcgcagagg ggcttctgtc
     2401 agtcatgccc gtcctccatc g
//
LOCUS       DASWHU010000259         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3109504, whole genome shotgun sequence.
ACCESSION   DASWHU010000259 DASWHU010000000
VERSION     DASWHU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109504"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VGB88_09545"
     CDS             complement(<1..1160)
                     /locus_tag="VGB88_09545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9647731.1"
                     /translation="MTKTFGPIAALLLATAILLLGNGLLGTLIPLRANIEAFPGPVIG
                     AIGSAYFGGFAAACLLVPYIVRRVGHIRSFVALTAAASAAPLLHLLAVAPVPWLVLRA
                     ITGFCMAGVYMVIESWLNERATNANRGLVLSVYTVVNLTALTGGQLLLMLFQPSAFQL
                     FCVGSVLVSLAALPVAMTTAVQPAPIASAKLRLRRLYRASPVGVVGCFAVGVANGPFW
                     ALGSVFAQDAGLDTRGIALFMSAVIVGGALLQWPFGRLSDHRDRRGVILALSLLSCLA
                     GAMLVVVRLGYAIPAVTPEAAVLIGGFLFGGFALPLYAISVAHANDFIGPAEFTEAAG
                     GLLLAYGIGATLGPVLAAALIGPWGSAVIFAFTAAVHAAFTLFVLWRMFRRRP"
     gene            complement(1183..2226)
                     /locus_tag="VGB88_09550"
     CDS             complement(1183..2226)
                     /locus_tag="VGB88_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9647732.1"
                     /translation="MSARWTRRPDGSNWGEFGADDQLGRLNLITPEKVRAGVAEVREG
                     RTFCLSLPLDFPGGNVLNPRRHPPRLAATLRAGRANYLYPFALENPDHIDVVCDDTVL
                     LTLQYSTQFDSLCHVGQAFDADGDGEAEPVFYNGWRGGEHVLGPPEDAAAGGPALAGV
                     HARRLGIENMAAHGVQGRGVMIDLERHVGRAREWIGYDRLMAICDADGVVVEAGDMVC
                     LYTGFADLILDMNRDPDGARLQQSCAVLDGRDQRLLQWITDSGLAVLIADNYAVEGSP
                     SRPPDRPGRHAALPLHQHCLFKLGIHLGELFHLGELARWLRVHQRNRFLLTAPPLRLP
                     GAVGSPATAIATV"
     gene            complement(2223..3023)
                     /locus_tag="VGB88_09555"
     CDS             complement(2223..3023)
                     /locus_tag="VGB88_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9647733.1"
                     /translation="MNRSEVERAAAALAGALGDRRRLDALPEDARPASEADAYRIQDA
                     LIAQLGAEPAGWKIGATSRMAQEMLKVGSPFAGRLLARSTYHSPARIPAGLMFMRGLE
                     VEFAFRIGRDLTAGDAPFDQAAVAEAVAALHPAIEIVDSRYHDWLAAGGLQLIADNGC
                     HGALVLGQAVTDWRRHDLAAVETELRLNGESVAAGAGRNVLGHPLAALTWLANHLAGR
                     GGGLAEGELVTTGSTCSGLGWAKAGDRGEGRFGPLGVVEVAFTDGPAS"
     gene            3386..>3631
                     /locus_tag="VGB88_09560"
     CDS             3386..>3631
                     /locus_tag="VGB88_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX9647734.1"
                     /translation="MALSVAHAHAHPPEPTTDTTVESVVIRFAGDSGDGMQLTGSQFT
                     LATAIQGNDLATFPDFPAEIRAPAGTTFGVSAFQINFS"
BASE COUNT          572 a         1313 c         1308 g          438 t
ORIGIN      
        1 gggcggcggc ggaacatgcg ccacagcacg aacagggtga aggcggcatg caccgcggcg
       61 gtgaaggcga agatcaccgc gctcccccac ggcccgatca gcgccgccgc cagcaccggg
      121 ccgagggtgg cgccaatgcc gtaggcgagc agcagcccgc cggcagcctc ggtgaactcg
      181 gccgggccga tgaagtcgtt ggcgtgggcg acgctgatcg catagagcgg cagcgcgaag
      241 ccgccgaaca ggaagccgcc gatcagcacc gccgcttcgg gcgtcaccgc cggaatggcg
      301 tagcccaggc gcacgaccac cagcatcgcc ccggcgaggc acgacaacag cgacagcgcc
      361 aggatcacgc cgcgccggtc gcggtggtcc gacagccgcc cgaacggcca ctgcagcagc
      421 gcgccgccga cgatcaccgc cgacatgaac agcgcgatgc cgcgggtgtc gagtccggcg
      481 tcctgggcga acaccgagcc gagcgcccag aacggcccgt tggccacgcc gaccgcgaag
      541 cagccgacca cgcccaccgg cgaggcgcgg tagaggcggc gcagcctgag cttggcagag
      601 gcgatcggtg ccggctgcac cgcggtggtc atcgccaccg gcagcgccgc cagcgacacc
      661 agcaccgagc cgacgcagaa cagctggaag gcggacggct ggaacagcat cagcagcagc
      721 tggccgccgg tcagcgccgt caggttgacc accgtgtaca ccgacagcac caggccgcga
      781 ttggcgttgg tcgcgcgctc gttgagccag ctctcgatga ccatgtagac gccggccatg
      841 cagaagccgg tgatcgcgcg cagcaccagc cacggcaccg gcgcgaccgc gagcaggtgc
      901 aggagcggcg ccgccgacgc cgccgcggtc agcgcgacga acgagcggat gtggccgacc
      961 cggcggacga tgtaggggac cagcaggcag gcggcggcga agccgccgaa ataggccgag
     1021 ccgatggcgc cgatcaccgg ccccgggaac gcctcgatgt tggcgcgcaa ggggatcagc
     1081 gtgcccaaca gcccgttgcc gagcagcagg atcgcggtcg cgagcaacag ggccgcgatc
     1141 gggccgaagg tcttggtcat cggcggcgcg cgcgggcgcg gatcagacgg tggcgatggc
     1201 agtcgccggc gagccgaccg cgcccggcaa cctgagcggc ggcgcggtga gcaggaagcg
     1261 gttgcgttgg tgcacgcgca gccagcgcgc gagctcgccc aaatggaaca gctcgccgag
     1321 atggatgccg agcttgaaca ggcagtgctg gtgcagcggc agcgcggcgt ggcggcccgg
     1381 ccggtccggc ggccgcgacg ggctgccctc gaccgcgtag ttgtcggcga tcagcaccgc
     1441 gagccctgag tcggtgatcc actgcaacag ccgctggtcg cgcccgtcga gcaccgcgca
     1501 ggactgctgc agcctggcgc cgtcggggtc gcggttcatg tccaggatca ggtcggcgaa
     1561 gccggtgtac aggcacacca tgtcgcccgc ctcgaccacc acgccgtcgg cgtcgcagat
     1621 cgccatcagc cggtcgtagc cgatccattc gcgcgcccgc ccgacatggc gctcgaggtc
     1681 gatcatcacg ccgcggccct ggacgccgtg ggcggccatg ttctcgatgc cgaggcggcg
     1741 cgcgtgcacg ccggcgagcg cggggccgcc ggcggccgcg tcctcgggcg ggccgagcac
     1801 gtgctcgccg ccgcgccagc cgttgtagaa caccggctcg gcctcgccgt cgccgtcggc
     1861 gtcgaacgcc tggccgacgt ggcacaggct gtcgaactgg gtcgagtact ggagcgtgag
     1921 cagcacggtg tcgtcgcaca ccacgtcgat gtggtcgggg ttctcgagcg cgaacgggta
     1981 caggtagttg gcgcgcccgg cgcgcaaggt ggccgccagc ctgggcgggt ggcggcgcgg
     2041 gttgagcacg ttgccgcccg gaaagtcgag cggcaggctg aggcagaacg tgcgcccctc
     2101 acggacctcg gcgacgcccg cacgcacctt ctcgggcgtg atcaggttga gccggccgag
     2161 ctggtcgtcg gcgccgaact cgccccagtt cgagccgtcg ggacggcgcg tccagcgcgc
     2221 gctcatgacg ccggcccgtc ggtgaacgcg acctcgacca cgcccagcgg gccgaaccgc
     2281 ccctcgccac ggtcgccggc cttggcccag ccgaggcccg agcaggtgct gccggtggtt
     2341 accagctcgc cctcggccaa gccgccgcca cgccccgcca ggtggttggc gagccaggtc
     2401 agcgcggcca gcggatggcc gagcacgttg cgcccggcgc cggcggcgac cgattcgccg
     2461 ttgagcctga gctcggtctc gaccgccgcc aggtcgtggc ggcgccagtc ggtgaccgcc
     2521 tgccccagca ccagcgcgcc gtgacagccg ttgtcggcga tcagctgcag gccgccggcc
     2581 gcgagccagt cgtgatagcg cgaatcgacg atctcgatcg cgggatggag cgcggcgacg
     2641 gcctcggcca ccgctgcctg gtcgaagggt gcgtccccgg cggtgaggtc gcggccgatg
     2701 cggaacgcga actcaacctc gagcccgcgc atgaacatga gtccggcggg gatgcgcgcc
     2761 gggctgtggt aggtcgagcg cgcgagcagc cggccggcga acggcgagcc gaccttgagc
     2821 atctcctgcg ccatgcggct ggtggcgccg atcttccagc ccgccggctc ggccccgagt
     2881 tgggcaatga gcgcgtcctg gatgcgatag gcatccgcct cgctcgccgg gcgtgcgtcc
     2941 tcgggcaggg cgtcgagccg gcggcggtcg ccgagcgcgc cggcgagcgc cgccgcggcg
     3001 cgctcgactt cgctgcgatt catgcctatc tccctgtcat gtccccggcc tattcaacag
     3061 catggccgaa ggcgcggcaa tgagcctctc ggagcccgcg cccggcccga aaatcgaccc
     3121 gcaaacaaca acttgtgacc aattgccggt agcgggccat ggccgcttgt attatagatt
     3181 tgctgaaact aactattgcg gcggcggcgg gtcgcgctgc cgggaggggg cattcgcgac
     3241 agccgcagcc cgcccggccg actgcccggt gccgacctaa cgtctcgaac gcctgcgcgg
     3301 aaccggcctc gcatggacgc gcgaggtaat tctgcaggcc cgctcaaggg ccggaggtca
     3361 cggcaccgcc cgtcgacagg gaatcatggc actcagcgtc gcccacgccc acgctcatcc
     3421 gcccgagccg accactgaca cgaccgtcga gtcggtggtg atccgcttcg ccggcgactc
     3481 cggcgacggc atgcagctca ccggcagcca gttcacgctg gcgaccgcca tccagggcaa
     3541 cgacctcgcc accttccccg acttcccggc cgagattcgc gcccccgccg gcaccacctt
     3601 cggcgtgtcc gcgttccaga tcaacttctc g
//
LOCUS       DASWHU010000260         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_131821, whole genome shotgun sequence.
ACCESSION   DASWHU010000260 DASWHU010000000
VERSION     DASWHU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131821"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="recR"
                     /locus_tag="VGB88_09565"
     CDS             complement(<1..578)
                     /gene="recR"
                     /locus_tag="VGB88_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9647735.1"
                     /translation="MAGAEIERLIQALARLPGLGPRSGRRAALALLAKRESLLRPLAE
                     ALAAADRTVRPCAACGNLDSTDPCAICADPKRDASLLCVVEGVAEVWALERAGAFRGR
                     YHVLGGLLSPLDGIGPEDLNISALVARAAAPEVVEVILALSATVDGQTTAHYVAERLA
                     GSGVAVTRLAHGVPVGGELDYLDDGTLTAALKA"
     gene            complement(601..921)
                     /locus_tag="VGB88_09570"
     CDS             complement(601..921)
                     /locus_tag="VGB88_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9647736.1"
                     /translation="MMSLGNLLKQAQAMQGRIEEMQQKLAEIEVEGRAGGGLVVATMN
                     GKGELKRLRVDPSLARADEVEVLEDLVVAACAEAKAKVEALAGEEMRKVTGGLPLPPG
                     LKLF"
     gene            complement(929..2647)
                     /locus_tag="VGB88_09575"
     CDS             complement(929..2647)
                     /locus_tag="VGB88_09575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9647737.1"
                     /translation="MNDRPESTATSEVPAAEGGYRVLARKYRPALLADLVGQEVMVRT
                     LANAFATGRIAHAFLLTGVRGIGKTTTARIIARALNCTGPDGSYQAPVAEPCGRCDDC
                     RAIAEDRHVDVLEMDAASHTGVENMRDLIDRVRYAPAIGRFKVYIIDEIHMLSVPAFN
                     ALLKTLEEPPAHVKFVFATTEPRKIPVTVLSRCQRFDLRRVDAEVLAGHLAAIAAKEG
                     AEVEDGALALIARAADGSVRDGLSMLDQAIGDAAPGPVQASQVREMLGLADRVRVFDL
                     FEAVLGGRLATALDLLGEQYRAGADPVTVLQDLLELTHWLTRVAVTPEAAADPGLPEA
                     ERVRGRAMAEALGVPELARAWQVLLKGLREAQAAPQPLEAAEMVLVRLAHAADLPTPA
                     DLVRTLQAGEAAPAPGPDRGRPPSGGGASARPARPRAAAAAPLAEPAPARAPERAALP
                     ETFEAVVALFADRREAMLAAHLAADVHLVGFEPGRIEWRPAAAAPANLASAVSQRLGD
                     WTGRRWVVAVSNEPGAPTLAEARAAGAEELRAEAARDPLVQSIIERFPGASIRAVRSG
                     PERSED"
     gene            complement(2677..2772)
                     /gene="ffs"
                     /locus_tag="VGB88_09580"
     ncRNA           complement(2677..2772)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGB88_09580"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3199..>3659
                     /locus_tag="VGB88_09585"
     CDS             3199..>3659
                     /locus_tag="VGB88_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX-like domain-containing protein"
                     /protein_id="HEX9647738.1"
                     /translation="MPTSNFYSGMPLARDEALRRDAAALARLCADPNSRVLAVWRTRH
                     QVTGTDAPRPVWHSGAPALSLLAESETWTLLGVAGGIAHFAVDVSHIEEPEAAPPLAG
                     RGSFVDLRSIGPLLSRAEGSVMAYARGLLYWHARHRFCGVCGSATESIHGG"
BASE COUNT          464 a         1379 c         1354 g          462 t
ORIGIN      
        1 gctttcagcg cggcggtgag cgtgccgtcg tcgagatagt cgagctcgcc gcccacgggc
       61 acgccgtggg cgaggcgggt gaccgcaacc ccgctgcccg cgaggcgctc ggcgacgtaa
      121 tgggcggtgg tctggccgtc gacggtggcc gacagcgcca ggatcacctc gacgacctcg
      181 ggggcggcgg cgcgcgcgac cagggccgag atgttgaggt cctcgggccc gatgccgtcg
      241 agcggcgaca gcaggccgcc gagcacgtgg tagcggccgc ggaaggcgcc ggcgcgctcg
      301 agtgcccaca cctccgcgac gccctcgacc acgcacaaca gcgacgcatc gcgcttgggg
      361 tcggcgcaga tcgcgcacgg gtcggtcgaa tcgagattgc cgcaggccgc gcacggccgc
      421 accgtccggt cggccgcggc cagcgcctcg gcgagcgggc gcagcagcga ctcgcgcttg
      481 gccagcaacg ccagcgccgc gcgccgcccc gagcgcggcc cgagcccggg caggcgcgcc
      541 agcgcctgga tcagccgctc gatctcggcc ccggccacga acgaacgtca accaagcggt
      601 tcaaaacagc ttcaggcccg gcggcagcgg cagcccgccg gtgaccttgc gcatctcctc
      661 gccggccagc gcctcgacct tggccttggc ctcggcgcag gccgcgacca ccaggtcctc
      721 gagcacctcg acctcgtcgg cgcgggcgag cgaggggtcg acgcggagac gtttcagctc
      781 gcccttgccg ttcatggtcg ccaccaccag gccgccgccg gcgcggccct cgacctcgat
      841 ctcggcgagc ttctgctgca tctcctcgat gcgcccctgc atcgcctgcg cctgcttcaa
      901 caggttgccg aggctcatca cgggcctctc agtcctcgct ccgctcgggg cctgagcgca
      961 cggcgcggat gctggcgccc gggaagcgct cgatgatgct ctgcaccagc gggtcgcggg
     1021 cggcctccgc gcgcagctcc tcggcgccgg cggcgcgcgc ttcggcgaga gtcggcgcgc
     1081 cgggctcgtt ggacaccgcc accacccagc gccgcccggt ccagtcgccg agccgctggc
     1141 tgaccgccga ggcgaggttc gccggcgccg ccgcggccgg ccgccactcg atgcgcccgg
     1201 gctcgaagcc gaccaggtgg acgtcggcgg cgagatgggc ggcgagcatc gcctcgcggc
     1261 ggtcggcgaa cagcgccacc accgcctcga aggtctcggg cagcgccgcg cgctcgggcg
     1321 cgcgcgccgg tgccggctcg gcgagcggcg cagcggccgc cgcccggggt cgtgccgggc
     1381 gcgccgaggc gccgccgccg gacggcggcc ggcctcgatc cgggccggga gcgggcgcgg
     1441 cctcgcccgc ctgcagcgtg cgcaccaaat cggccggcgt cggcaggtcg gcggcatggg
     1501 caaggcgcac cagcaccatc tccgccgcct ccagcggctg cggcgccgcc tgcgcctcgc
     1561 gcaggccctt gagcagcacc tgccaggcgc gggcgagctc gggcacgccg agcgcctcgg
     1621 ccatcgcccg gccgcgcacg cgctcggcct cgggcaggcc cgggtcggcg gcggcctcgg
     1681 gcgtgaccgc gacccgggtc agccagtggg tcagctccag caggtcctgg agcacggtca
     1741 ccgggtcggc gccggcgcgg tactgctcgc cgagcaggtc gagcgccgtt gccagccggc
     1801 cgccgagcac cgcctcgaac aggtcgaaca cgcgcacccg gtcggcgagc cccagcatct
     1861 cgcgcacctg gctcgcctgc accggccccg gcgcggcgtc gccgatcgcc tggtcgagca
     1921 tcgacagccc gtcgcgcacc gagccgtcgg cggcgcgcgc gatcagcgcc agcgcgccgt
     1981 cctcgacctc ggcgccctcc ttggcggcga tcgcggccag gtggccggcg agcacctcgg
     2041 catcgacccg gcgcaggtcg aagcgctggc agcgcgacag cacggtgacc gggatcttgc
     2101 gcggctcggt ggtcgcgaac acgaacttca catgcgccgg cggctcctcg agggtcttca
     2161 acagggcgtt gaacgccggc accgacaaca tgtggatctc gtcgatgatg tagaccttga
     2221 accggccgat agccggcgca tagcggaccc ggtcgatgag gtcgcgcatg ttttcgacgc
     2281 cggtgtggct ggcggcatcc atctcgagca cgtcgacgtg gcggtcctcg gcgatcgccc
     2341 ggcagtcgtc gcagcgcccg cacggctcgg cgaccggcgc ctggtacgag ccgtcggggc
     2401 cggtgcagtt gagcgcgcgg gcgatgatgc gcgcggtcgt ggtcttgccg atgccgcgca
     2461 cgccggtgag caggaaggca tgcgcgatcc gcccggtggc gaaggcgttg gcgagggtgc
     2521 gcaccatcac ctcctggccg accaggtcgg cgagcagcgc cgggcggtac ttgcgcgcga
     2581 gcacgcgata gccgccctcg gcggcgggca cctcgctcgt ggctgtggac tcggggcggt
     2641 cgttcatcgg ggcgctggca cgggcatcgg actcgggtgg gagaccggac cacgacccgg
     2701 gcctcactcg ttacggctgc ttccttccgg acctgaccgg gttggcgagc ggcccgtccg
     2761 cggccggcct cccgggcgca ctatcggcca tcgccccgcc cggggcaagc cggccggcga
     2821 gccaggggcg ggcacgcggc cttcggtcgg tcacgcgatt cgagtttggg ctcgcggagc
     2881 cgcgaaggac gcggaggagg gttgaaacac agaggactca gaggacacag aggcccagga
     2941 cgtatctccg tcgtcattcc cgcgaaagcg ggaatccaga ttttccctga gttgctggac
     3001 ccccgcttgc gcgggtgtga cgggcccatc ggcgtccgtc tcaggatcga aggcgggaac
     3061 acagggactg cggcgcggct ggcgcgccct ccgcgtcctc cgcggctccg cgtgccaaac
     3121 cttctcgctg gcgctcgggc ggggtgttcg gcgggcgggc tgggatataa tgcgccgccc
     3181 cagacgtgga ttcacgtgat gcccacgagc aacttctaca gcggcatgcc gctcgcgcgc
     3241 gacgaggcgc tgcgccgcga cgccgccgcg ctcgcccgcc tctgcgccga ccccaacagc
     3301 cgcgtgctcg cggtgtggcg cacccgccac caggtgaccg gcaccgatgc cccgcgcccg
     3361 gtctggcaca gcggcgcgcc cgcgctctcg ctgctcgccg aaagcgagac ctggacgctg
     3421 ttgggagtgg ccggcggcat cgcccatttc gccgtcgacg tctcgcatat cgaggagccc
     3481 gaggcggcgc cgcccttggc cgggcgcggc agcttcgtcg acctgcgcag catcggcccg
     3541 ctgctgtcgc gcgccgaggg ctcggtgatg gcgtatgcgc gcgggctgct ctactggcac
     3601 gcccgccacc gcttctgcgg ggtgtgcggc agcgccaccg agagcatcca cggcggcca
//
LOCUS       DASWHU010000261        17460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_876608, whole genome shotgun sequence.
ACCESSION   DASWHU010000261 DASWHU010000000
VERSION     DASWHU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876608"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VGB88_09590"
     CDS             complement(<1..560)
                     /locus_tag="VGB88_09590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016858568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9647739.1"
                     /translation="MARKNTRMKSISLTPEVYDYLLEVSLRESEVQRRLRAETESVPM
                     ALMQVPPEEAQLLALLVELIDARRCLEIGVFTGYSALAVAMALPADGRVVALDKDAET
                     AAVARRAWAEAGVAARIDLRLGDAVKTLDRLIAAGEAGSYDFAFLDADKSNYPRYWEQ
                     ILVLLRPGGLIAVDNVLWGGAVIDMRR"
     gene            664..2388
                     /locus_tag="VGB88_09595"
     CDS             664..2388
                     /locus_tag="VGB88_09595"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX9647740.1"
                     /translation="MGNGHDTGLRKGLTHYGDAGFSLFLRKSFAKAMGYSDADLDRPV
                     VGITNTASGFNNCHQTVPALIEAVKRGVMYGGGLPVEFPTISLGEPYLAPTSMLFRNL
                     MSIDTEEMIRAHPLDAVVLVGGCDKTVPAQLMAAASANLPAISLVTGPMMTSSWRGER
                     LGACTDCRRQWGRFRAGELDEAEIAEVGGTLSTTAGTCMVMGTASTIALMTEALGMML
                     AGGAAIPAVNAERLRLAEATGARAVAMAGEGLVPSRILTPAAFANAFRVLLAIGGSTN
                     GLIHMTAIAGRAGIAVDLDGIDRMGRETPVLVDLKPSGAHYMEDLYKAGGLAPILREL
                     RPLLNLDCLTVSGRTLGAELDAGPPAWPQTVVRPFAEPLAREGGIAVLRGNLAPDGAI
                     IKQSAASPALLTHEGRAVVFDGLDDLARRIDDDALEVAPEDVLVLRNAGPKGAPGMPE
                     SGYVPIPRKLARAGVKDMVRISDARMSGTAFGAIVLHVAPEAAVGGPLALVETGDRIR
                     LDVPRRALALLVDEETLAARRAAWRPPAPHPGAERGWLKLHLDQVGQADTGCDLDVLR
                     PPPTATTP"
     gene            complement(2398..2799)
                     /locus_tag="VGB88_09600"
     CDS             complement(2398..2799)
                     /locus_tag="VGB88_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9647741.1"
                     /translation="MPATALDHVTVVTKDLQASRDFYIGVLGLEEADFRPDFPFDGAW
                     LTLGERAVVHLVVMDSRERGDGNTQPFDHFALRAQNMTGMRKTLAKHRIAFEEAAPPN
                     STIRQFFLHDPDGVQVELTFDATAEKRAGTL"
     gene            complement(2831..4021)
                     /locus_tag="VGB88_09605"
     CDS             complement(2831..4021)
                     /locus_tag="VGB88_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9647742.1"
                     /translation="MEFSLDAEIVLPEDGTDGTLIGRCMVPGADSAPGGPSVVAIADS
                     GVWDISGAFATSAALMDAPNPAAALASTASAVRLGSIDEVLANTVPDDRDPTRPWFLA
                     PIDLQAIKAAGVTFLDSLMERVIEELAKGDPDQAEAIRGSIAKEIGTSLTGIRPGSDE
                     AMRLKESLIRRDLWSPYLEVGIGPDAEIFTKAQPMSAVGVGAEIGIPEHSTWNNPEPE
                     AVLVVNARGEIVGATLGNDVNLRDVEGRSALLLGRAKDNNASCAIGPFIRLFDTTFGL
                     DDLTGVEISLAVEGEDQFSLVGSSSMALISRAPRDLVAQTINRDHQYPDGLVLLTGTM
                     FAPTKDRTEPGRGFTHAPGDVVVIATPPLGALINRVTYCHQAEPWTFGVGALMANLAA
                     RGLL"
     gene            4218..4547
                     /gene="hspQ"
                     /locus_tag="VGB88_09610"
     CDS             4218..4547
                     /gene="hspQ"
                     /locus_tag="VGB88_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX9647743.1"
                     /translation="MQIRLAKFQIGDIVRHRFYPFRGVIFDVDPEFANTEEWYQSIPA
                     ELRPRKDQPFYHLLAENEQTTYIAYVSEQNLLVDDSGKPVTHPDAKLMFTGIRDGRYV
                     RRSGHAN"
     gene            complement(4562..6250)
                     /locus_tag="VGB88_09615"
     CDS             complement(4562..6250)
                     /locus_tag="VGB88_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HEX9647744.1"
                     /translation="MHSLPPFLIFVVGALVLPLVAARHRPALTLLVPLAGFVNLLMLD
                     SGNHWQVAMLGYTLEFGRVDKLSFLFAFLFHLAAGLGAIFALHVRDAMQQVAALLYAG
                     GAVGAVFAGDLVTLFIFWEVMAVSSAFLIFARRTEHASRAGLRYLIAQITSGVLLLAG
                     AVIHQGETGSLAFDLLDLSGLGAKLIFIAFGIKCAWPLIHAWLPDAYPEATATGTVYL
                     SAFTTKFAVYGLARAFPGTELLIYIGTAMTMFPIFFAVIENDLRRVLSYSLINQVGFM
                     VVGIGLGTTLALNGTVSHAFNHVIYKALLFMSMGAVLHMTGRIHATDLGGLYKSMPLT
                     TTFCIIGAASISAFPLFSGFVSKSMVLASAAEEGHVVVFLLLLFASAGVVEHAGIKVP
                     YFAFFAHDSGIRTTEPPVNMLIAMGFAAFICVFNGVWPWLLYDFLPWPVDYAPYTPTH
                     VIAQAQLLLFAVLAVVLLMVSGLYPPEMRLVNLDSDWLVRRLGKRLAVGAGRAVMAAW
                     SGLLDAAQGALGGLISHLYQAHGPRGVLARTWPTGSTVMWIAALLAIYLLINYA"
     gene            complement(6243..6512)
                     /locus_tag="VGB88_09620"
     CDS             complement(6243..6512)
                     /locus_tag="VGB88_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647745.1"
                     /translation="MPPEGRRYWLDDWRNVRKLLFAFFAVCALLILLDLLYHKHAVLA
                     FEEWFGFYAIYGFVACMILVLASRLLRKLVGRRKDYYQGGARDDA"
     gene            complement(6526..7929)
                     /locus_tag="VGB88_09625"
     CDS             complement(6526..7929)
                     /locus_tag="VGB88_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9647746.1"
                     /translation="MIGLAGARPNLREAVTLVTAGALFATVAWVAPAVMAGARPEAEL
                     YDVLPGIALAFRAEPLGMLFALIASFLWIPNSVYSIGYMRAHHEQNQTRFYVCFAVAL
                     AGAVGVAFAANLFTLFLFYELLTLSTYPLVTHSGTDEARRSGRLYLGTLLGTSIGFQL
                     LAILATYALAGTLDFRPGGILAGKAEPGVLAILFALYMFGIGKAAIMPFHRWLPAAMV
                     APTPVSALLHAVAVVKAGVFTVMKVVIYVFGLDLMQGANPGAWLPWLATFTLLAASAI
                     ALTKDNLKAMLAYSTVSQLAYIVLAASLATSSAIVGGGLHMLMHAFGKITLFFCAGAI
                     LITAHKENISDMRGLGRAMPLTMFAFLLGSLSVIGLPPFAGAWSKWFLALGAVEAHQL
                     VFVAALMVSSLLNVGYFMPVVVRAFFPGTGPTEFDGGVREAPLACLVPLLFTAAACVL
                     LFLFPDPAYRLLLQLVP"
     gene            complement(7983..9461)
                     /locus_tag="VGB88_09630"
     CDS             complement(7983..9461)
                     /locus_tag="VGB88_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HEX9647747.1"
                     /translation="MIGLNLPALQVVVPLFGAPLCTLIGRGARAWGFSLLVTWTAFAL
                     AIALMAKVAADGPISYMMGDWPAPWGIEYRVDFLNAFVALFVTGVAAAVIPFAYHSVA
                     AEIPADRHRLFYTAYLLTFAGLLGMTVTGDAFNAFVFMEISSLSGYVLVSLGRDRRAL
                     FASYQYLVLGTIGATFFVIAIGLLYIATGTLNMADLAARIPALEGNLVVIAAYAFLLV
                     GLSLKVALFPLHLWLPNAYTYAPSMVSALLAATATKVGIYLILRFMFGVFGVGFSVGA
                     HPVTILLMPLALIAAVSGSLVAITQINVKRVLAYSSVAQIGYIVIGIAFATSTGLTGA
                     VVHLFNHALMKGAMFLALGAVMLRVGGTRIEHLKGLGRRMPLTMAAFVAAGLSMIGVP
                     GTVGFVSKWYLVLAALEAGWWPVAVVIIGTSILAIVYVWRVVEAAYFAEPDNPSDVAE
                     APWPMVAATWALAGACILFGLTTELTVGTARLAAGMLLGTAP"
     gene            complement(9458..9823)
                     /locus_tag="VGB88_09635"
     CDS             complement(9458..9823)
                     /locus_tag="VGB88_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HEX9647748.1"
                     /translation="MALLGEYAYWVVVLVMMIGFFVVIDRGNLLKKLIGLNIFQTAVF
                     LLYISSGKIFGGTAPIIYDGGAPYSNPLPHVLILTAIVVGVATLALGLALVVRIKEAY
                     GTVEEDEIQAIETREEDAA"
     gene            complement(9830..10255)
                     /locus_tag="VGB88_09640"
     CDS             complement(9830..10255)
                     /locus_tag="VGB88_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HEX9647749.1"
                     /translation="MTELLILRVVAKFLIPFILLFGLYVQFHGDFGPGGGFQAGVIIA
                     AGCVLYGLVFGLDNLRAAIPPPLVRVLVAVGLSIYAGTGVLTVLLGGRFLDYQWLAHD
                     PRHGHHYGIMIVEFGVGTTVAAVMLAIYCSFAGRGEPER"
     gene            complement(10252..10776)
                     /locus_tag="VGB88_09645"
     CDS             complement(10252..10776)
                     /locus_tag="VGB88_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008187376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HEX9647750.1"
                     /translation="MLVVTALTIVRMRSILAVVMLSGIYSFLMASIFLVLDAVDVAFT
                     EAAVGAGVTTVLGLVALSLTGDQEKIPRRRPVLALAVVIVTGVVLLYATEDMPPIGDP
                     AAPIHHHVVPRYLAETPEAIGIPNVVTSVLADYRGYDTLGEVAVIFTAGVGVMLLLGR
                     RRRDPAAGEGKDDQ"
     gene            complement(10806..11153)
                     /gene="mnhG"
                     /locus_tag="VGB88_09650"
     CDS             complement(10806..11153)
                     /gene="mnhG"
                     /locus_tag="VGB88_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX9647751.1"
                     /translation="MSLVADVLSWACMLAGALFIMVGGIGLVRLPDFYSRAHAAGMTD
                     TLGAGLMIASMMLESGWDLNLARLAFIFLFLLFTSPTASHALTHSALVSGLKPWTRPD
                     GERGGGAAAEGER"
     gene            complement(11150..11392)
                     /locus_tag="VGB88_09655"
     CDS             complement(11150..11392)
                     /locus_tag="VGB88_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEX9647752.1"
                     /translation="MALAVARAVAGPTVFDRILAANAFGTKTVLLIAVLGYLTGRPEF
                     LDLGLVYALINFLGVIAALKYFKFGRFDDRRAGQRR"
     gene            complement(11434..11907)
                     /locus_tag="VGB88_09660"
     CDS             complement(11434..11907)
                     /locus_tag="VGB88_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX9647753.1"
                     /translation="MHTVSLFAALAAVWLLLSGHYEPLLLGFGAASCAFVCALARRFD
                     VVDRESHPLHMSLLLPGYWLWLLVEIVKANIDVARRVIDPKLPIDPRLFEVRTSQRTD
                     LGRVIYANSITLTPGTISVDLAPDRILVHALAAEGEAALLEGTMDRKVTALEGGA"
     gene            complement(11948..12886)
                     /locus_tag="VGB88_09665"
     CDS             complement(11948..12886)
                     /locus_tag="VGB88_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647754.1"
                     /translation="MGRAFAALVAISIAAAGPAGAADKLTLVLDWFVNPDHAPLLVAQ
                     ENGYFAAQGLEVELIAPADPNDPPKLVAAGKADLAVSYQPQLHIQVAEGLPLVRVATL
                     VATPLNCLVVLADGPIRTIADLKGRKVGFSVGGFEDALLGAMLTASGLALDDVTLINV
                     NFSLSPAVISGQVDAVIGAFRNFELNQMDLAGHPGRAFYVEEHGVPAYDELIVVANRA
                     RLDDSRLGRFVAALEQGVQFLVNHPDESWALFVRGRPELDDELNRRAWRDTLPRFALR
                     PRALDALRYVRFSRFLADLGLIAEARPADSYAVVIE"
     gene            complement(12954..13961)
                     /gene="egtD"
                     /locus_tag="VGB88_09670"
     CDS             complement(12954..13961)
                     /gene="egtD"
                     /locus_tag="VGB88_09670"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9647755.1"
                     /translation="MPDQSPVPDVDDRIDCRTVAATRRVSSLLDDVRSGLLSAPRSLP
                     PKYFYDQRGSALFDRICDTPEYYLTRTEDALLDASAAAIIAEAAPDDILELGSGMARK
                     TRRLLDACGAAGMRPTYSPFDISRAALMRAARELADEYSWLRVRVLVGDYDAGLAHLP
                     PARGRRLFCFLGSTIGNFEHDKAVAFLSDLRARMNGGDLLLLGVDRVKDEAVLHAAYN
                     DRAGLTAAFNLNILRVLNAGAEADFDLDQFDHRAGFDRRLSRIEMHLVSRRDQDVDLG
                     ALGTSIHIAAGEAILTEISRKFTPESLAAMLRAAGFTFDFHYQPANGYFSLVLARPVR
                     A"
     gene            14091..14864
                     /gene="egtC"
                     /locus_tag="VGB88_09675"
     CDS             14091..14864
                     /gene="egtC"
                     /locus_tag="VGB88_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HEX9647756.1"
                     /translation="MCRHAAYLGPPIALNRFLAEPSHSLMVQSWRPREQITATVNADG
                     FGFGWYLDDGRAGVYANPMPIWSDPNLDHLGAALSSRLWIGNVRSATRGQPVAQVNTH
                     PFLAGRELFSHNGFVHDFANSLRGTLRRHLEPAFEAQIRGSTDSEYLFAALRQETAAG
                     GGTAAALRRLFARLEAWLAGSHGLFNLLIADGERIYATRHALSHASPSLYVGGDAELF
                     DGGWLVASEPLTGGGGWQEVPDHHLVVIEAGREPRLEPL"
     gene            14861..16015
                     /locus_tag="VGB88_09680"
     CDS             14861..16015
                     /locus_tag="VGB88_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9647757.1"
                     /translation="MSGAARARLRALQAVLQATVADCGEADARTQFDPALSPLGWHLR
                     HCAFVEALWIRERVLGDDRLTAPLAEDCLPERVPKEARGESLPGHQALLDWTATTMAD
                     NLELLGDAERRRERHGLLADGYLWDFLIGHHAQHLETMRMVRAARAVAGAGGGTTGGA
                     VRAAVPDWEWRRLPAGRYEIGTEDGFGFDNEGTAQMVALAGVLIAATPVSNAQYLAFI
                     EDGGYDEKRRWSAAGWRWRTSSGVTCPHGWRRGAAGGWLEVGPDGAQALTPGAAVVGL
                     GRHEAGAFAAWAGAALPHEYQWEAAARLGLLERTGEAWEWCANAFHPYPGFRAHPYRE
                     YSAPWFDGRHGVLRGGSRYSEPEMRRPGFRNYYEPACRHVFAGLRLARPA"
     gene            complement(16012..16767)
                     /locus_tag="VGB88_09685"
     CDS             complement(16012..16767)
                     /locus_tag="VGB88_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647758.1"
                     /translation="MTALRGLAIAALLAAAWQALVWLAATPPYILPGPGRVASAWWIH
                     RATLLDHAGTTVIEILLGLLIGAALGVLSALVLAHFRTARGWLLPVLVVSQALPVFAL
                     APVLVLWLGYGLASKVAMAVLIIYFPVTATFYDGLRRTDAGWLDLAATMGASRFSALV
                     RIRVPAALPALASGAKVAAAVAPIGAVVGEWVGSSAGLGYLMLHANGRMQIDLMFAAL
                     VTLAVAAIAIYFAVDKLARAALPWLPETGPGPD"
     gene            complement(16764..17456)
                     /locus_tag="VGB88_09690"
     CDS             complement(16764..17456)
                     /locus_tag="VGB88_09690"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647759.1"
                     /translation="MLFEDLAVTLEAGRLTCLLGPSGVGKTSLLRLIAGLAPAGEEGR
                     VWCDDGRPAAGRVAYMAQSDLLLPWLTALENVMLGPRLRGERAPVAQARALIARVGLA
                     GHERARPAALSGGMRQRIALARTLLEDRPIVLMDEPFSALDAITRTRLQDLAVATLRG
                     RTVLLVTHDPLEALRIADRILVMAGRPATVGESLVPPGPPPRAIGAPDIAALHGDLMR
                     RLTAADTIELRP"
BASE COUNT         2872 a         6056 c         6262 g         2270 t
ORIGIN      
        1 cggcgcatat cgatcaccgc gccgccccac agcacgttgt cgaccgcgat cagcccgccc
       61 gggcgcagca gcaccaggat ctgctcccag tagcgcggat agttcgactt gtcggcatcg
      121 aggaaggcga agtcgtagga cccggcctcg ccggccgcga tcaggcggtc gagcgtcttg
      181 accgcatcgc cgaggcgcag gtcgatgcgc gccgccaccc cggcctcggc ccaggcgcgg
      241 cgcgcgaccg cggccgtctc cgcgtccttg tcgagcgcga ccacgcggcc gtccgcgggc
      301 agcgccatgg cgaccgcgag cgcgctgtag ccggtgaaca cgccgatctc gaggcagcgg
      361 cgggcatcga tcagctcgac cagcagcgcc aacagctgcg cctcctcggg cggcacctgc
      421 atcagcgcca tcggcacgct ctcggtctcg gcgcgcagcc ggcgctggac ctcgctctcg
      481 cgcagcgaca cctcgagcag gtagtcgtag acctcaggcg tcaggctgat cgacttcatc
      541 ctggtgttct tgcgcgccat ggcggcatcc tgcgtgcggc gtgggaacag cgatactgcg
      601 gtggacgctc cggccgggcg ccctcggact ggatcggttc ggactatgcg ggagaggcgg
      661 gcggtgggca acggtcacga cacgggcttg cgcaaggggc tcacgcatta cggcgatgcc
      721 gggttctcgc tgttcctgcg caagagcttc gccaaggcga tgggctattc ggacgccgac
      781 ctcgaccgcc cggtggtcgg catcaccaac accgccagcg gcttcaacaa ctgccatcag
      841 acagttccgg cgctgatcga ggcggtcaag cgcggcgtga tgtatggcgg cggcctgccg
      901 gtcgagttcc cgaccatctc gctcggcgag ccgtacctcg cgccgaccag catgctgttc
      961 cgcaacctga tgtcgatcga caccgaggag atgatccgcg cccacccgct cgacgcggtg
     1021 gtgctggtcg gcggctgcga caagaccgtg ccggcgcagc tgatggcggc ggcgagcgcg
     1081 aaccttcccg cgatctcgct ggtgaccggg ccgatgatga cctcgagctg gcgcggcgag
     1141 cggctcggcg cgtgcaccga ctgccgccgc cagtggggcc gcttccgcgc cggcgagctc
     1201 gatgaggccg agatcgccga ggtcggcggc accttgtcga ccaccgccgg cacctgcatg
     1261 gtcatgggca ccgccagcac gatcgcgctg atgaccgaag cgctcggcat gatgcttgcg
     1321 ggcggcgccg cgatcccggc ggtcaacgcc gagcgcctgc ggctcgccga ggccaccggc
     1381 gcgcgcgcgg tggcgatggc gggcgagggg ctggtgccgt cgcgcatcct gaccccggcg
     1441 gcgttcgcca acgccttccg cgtcctgctc gcgatcggcg gctcgaccaa cgggctgatt
     1501 cacatgaccg cgatcgccgg gcgcgccggc atcgccgtcg accttgacgg tatcgaccgc
     1561 atggggcgcg agacgccggt cctggtcgac ctcaagccgt cgggcgcgca ctacatggag
     1621 gacctgtaca aggcgggcgg gctggcgccg atcctgcgcg agctcaggcc gctcctgaac
     1681 ctcgactgcc tgacggtgag cgggcggacg ctcggtgccg agctcgacgc cgggccgccg
     1741 gcctggccgc agaccgtggt gcgcccgttc gccgagccgc tggcgcgcga gggcggcatc
     1801 gccgtgctgc gcggcaacct ggcgccggac ggcgcgatca tcaagcaatc ggcggcctcg
     1861 cccgcgctgc tcacgcacga gggccgggcg gtggtgttcg acggcctcga cgacctcgcc
     1921 cggcggatcg acgacgacgc gctcgaggtc gcgcccgagg acgtgctcgt gctgcgcaac
     1981 gccggcccca agggcgcccc gggcatgccc gagtcgggct acgtcccgat cccgcgcaag
     2041 ctcgcccgcg ccggggtcaa ggacatggtg cgcatctcgg acgcgcgcat gagcggcacc
     2101 gcattcggcg ccatcgtcct gcacgtcgcg cccgaggcgg cagtcggcgg gccgctggcg
     2161 ctggtcgaga ccggcgaccg catccgcctc gacgtgcccc ggcgcgcgct cgcgctgctc
     2221 gtcgatgaag aaacgctggc tgcccgccgc gccgcctggc ggccgcccgc gccgcatccc
     2281 ggcgcggagc gcggctggct caagctccac ctcgaccagg tcggccaggc cgacaccggc
     2341 tgcgatctcg acgtcctcag gccgccgccg accgcgacga cgccgtaagg cagcggctta
     2401 cagcgtgccg gcgcgcttct cggcggtggc gtcgaaggtc agctcgacct gcacgccgtc
     2461 ggggtcgtgg aggaagaact ggcggatggt cgagttgggc ggcgccgcct cctcgaaggc
     2521 gatgcggtgc ttggcgagcg tcttgcgcat ccctgtcatg ttctgcgcgc gcagggcgaa
     2581 gtggtcgaac ggctgggtat tgccgtcgcc gcgctcgcgg ctgtccatca ccactaggtg
     2641 caccaccgcc cgctcgccga gcgtgagcca ggcgccgtcg aatgggaagt caggcctgaa
     2701 gtcggcctcc tcgaggccca gcaccccgat gtagaagtcg cgggacgcct gcaggtcctt
     2761 ggtcacgacg gtgacgtgat cgagtgcggt cgcgggcatg tctgacacct ccgaaaacgt
     2821 gccgatcatg tcacaacagg ccgcgcgccg ccagattcgc catcagcgcg ccgacgccga
     2881 aggtccacgg ctcggcctgg tggcaatagg tgacccggtt gatcagcgcg ccgagcggcg
     2941 gcgtggcgat gaccaccacg tcacccgggg cgtgggtgaa gccgcggccg ggctcggtgc
     3001 ggtccttggt cggcgcgaac atggtgccgg tcagcagcac caggccgtcg ggatactggt
     3061 ggtcgcggtt gatggtctgc gcgaccaggt cccggggcgc gcggctgatc agcgccatcg
     3121 acgacgagcc gaccagcgag aactggtcct cgccctcgac cgccaggctg atctcgacac
     3181 ccgtgaggtc gtcgaggccg aaggtcgtgt cgaacaggcg gatgaacggg ccgatcgcgc
     3241 aggacgcgtt gttgtccttg gcgcgcccga gcagcagcgc actccgcccc tcgacgtcgc
     3301 gcaaattgac gtcgttgccg agggtggcgc cgacgatctc gccgcgggca ttgaccacca
     3361 gcaccgcctc gggctcgggg ttgttccagg tcgagtgctc ggggatgccg atctcggcgc
     3421 cgacgccgac cgccgacatc ggctgcgcct tggtgaagat ctcggcgtcg gggccgatgc
     3481 cgacctcgag atagggcgac cacaggtcgc gccggatcag cgactccttg agccgcatcg
     3541 cctcgtccga gcccggcctg atgccggtca ggctggtgcc gatctccttg gcgatcgagc
     3601 cgcggatcgc ctcggcctgg tcggggtcgc ccttggccag ctcctcgatc acccgctcca
     3661 tcaggctgtc gagaaaggtc acgccggcgg ccttgatcgc ctgcaggtcg attggtgcga
     3721 ggaaccacgg ccgggtcggg tcgcggtcgt cggggacggt gttggcgagc acctcgtcga
     3781 tgctgccaag gcgcaccgcg cttgcggtgc tggcgagggc ggcggccggg ttgggcgcgt
     3841 ccatcagcgc ggccgaggtg gcgaaggcgc cgctgatgtc ccacacgccg ctgtcggcga
     3901 tcgccaccac gctcggcccg cccggcgccg agtcggcgcc cggcaccatg cagcgcccga
     3961 tcagggtgcc gtcggtgccg tcctcgggca gcacgatctc ggcgtcgagg ctgaactcca
     4021 tggcccgcgt ctcccttgac ctcagggcgc gatattgcgg caatcgttgc ccgccgacaa
     4081 cggtcgcgcg ccgcggccgg cgcccccggc ggcggccctg ccggggtttg ggcggtaggt
     4141 tagatgcgct ggcgcaaggc cacggaatcg cttagcattc gccgtgagag gagggcccga
     4201 gcgcgggctt ccgggcgatg cagatcaggc tggccaagtt ccagatcggc gatatcgtgc
     4261 gccaccggtt ctatccgttc cgcggcgtga tcttcgacgt cgacccggag ttcgccaaca
     4321 ccgaggagtg gtaccagtcg atcccggccg agctgcgccc gcgcaaggac cagccgttct
     4381 accacctgct ggccgagaac gagcagacca cctacatcgc ctacgtctcg gagcagaacc
     4441 tgctggtcga cgacagcggc aagccggtca cgcacccgga cgccaagctg atgttcacgg
     4501 gcatccgcga cggccgctac gtgcgccgca gcggccacgc caactgagcc cgggcgctcc
     4561 ctcaggcgta gttgatcagc aggtagatcg cgagcagggc ggcgatccac atcacggtcg
     4621 agccggtcgg ccaggtgcgc gccagcacgc cgcgcgggcc gtgggcctgg tagaggtggc
     4681 tgatcagccc gccgagcgcg ccctgcgcgg catcgagcag ccccgaccac gccgccatca
     4741 ccgcccgccc ggcaccgacc gcgagccgct tgccgaggcg gcgcaccagc cagtccgagt
     4801 cgagattgac gaggcgcatc tccggcggat acaggcccga gaccatcagc agcacaaccg
     4861 cgagcaccgc gaacagcaga agctgggctt gggcgatcac atgtgttggc gtgtagggcg
     4921 cgtagtcgac cggccacggc aggaaatcgt agagcaacca tggccagacg ccgttgaaca
     4981 cgcagatgaa ggcggcaaag cccatcgcga tcagcatgtt gaccggtggc tcggtggtgc
     5041 ggatgccgga atcgtgcgcg aagaacgcga agtagggcac cttgatgccg gcatgttcca
     5101 ccacgccggc actggcgaac agcagcagca ggaacaccac cacgtggcct tcctcggcgg
     5161 cgctcgccaa gaccatcgac ttcgacacga agcccgagaa cagcgggaag gccgagatcg
     5221 aggcggcgcc gatgatgcag aaggtcgtgg tcagcggcat ggacttgtac agcccgccta
     5281 ggtcggtggc gtggatgcgc ccggtcatgt gcaggaccgc gcccatcgac atgaacagga
     5341 gcgccttgta gatcacgtgg ttgaaggcgt ggctgaccgt gccgttgagc gccagcgtgg
     5401 tgccgagccc gatgccgacc accatgaagc cgacctggtt gatcaggctg taggacagca
     5461 cgcgccgcag gtcgttctcg atcaccgcaa agaagatcgg gaacatggtc atcgcggtgc
     5521 cgatgtagat cagcagctcg gtgcccggaa atgcgcgcgc gaggccgtag accgcgaact
     5581 tggtggtgaa cgcactcaga tagaccgtgc cggtcgcggt tgcctccgga taggcgtcgg
     5641 ggagccaggc gtggatcagc ggccaggcgc atttgatgcc gaaagcgatg aagatcagct
     5701 tggcgccgag gcccgacagg tcgaggaggt cgaaggcgag gctgccggtc tcgccctgat
     5761 ggattacggc gcccgcgagc agcagcacgc ccgaggtgat ctgggcgatg aggtagcgca
     5821 gccccgcgcg gctcgcatgc tcggtgcggc gggcgaagat caggaacgcc gatgagaccg
     5881 ccatcacctc ccagaagatg aacagggtca ctaggtcgcc ggcgaacacc gcgccgaccg
     5941 cgccgccggc atagagcagc gccgcgacct gctgcatggc gtcgcgcacg tgcaaggcga
     6001 agattgcacc gagcccggcc gcgaggtgga acaggaacgc gaacaggaac gacagcttgt
     6061 cgacgcgtcc gaactcgagg gtgtagccga gcattgccac ctgccagtgg ttcccggaat
     6121 cgagcatgag caggttgacg aacccggcga gcgggacgag cagggtgagg gcggggcggt
     6181 ggcgcgccgc aaccaggggc agcaccagcg cgccgacgac gaagatcagg aacggcggca
     6241 ggctatgcat cgtcgcgcgc cccgccctgg taataatcct tgcgacgccc gaccagcttg
     6301 cgcagcagcc tggaggcgag cacaagaatc atgcacgcga cgaagccgta gatcgcgtag
     6361 aacccgaacc attcctcgaa cgccagtacc gcgtgcttgt ggtagagaag gtcgagcagg
     6421 atcaggagcg cgcacaccgc aaagaacgcg aacagcaact tgcgcacatt gcgccagtcg
     6481 tcgagccagt atctccgtcc ctcgggcggc atcttcggat cctcgctacg gcaccagttg
     6541 cagaagcaac cgatacgccg ggtctggaaa caaaaacaac agcacgcagg cggcggcggt
     6601 gaacagcaac ggcaccaggc aggcgagcgg cgcctcgcgc acgccgccgt cgaactcggt
     6661 cggccccgtg cccgggaaaa aggccctcac caccaccggc atgaagtagc cgacgttcaa
     6721 caggctcgag accatcagcg cggcgacgaa cacgagctgg tgggcttcca ccgcgccgag
     6781 ggcgaggaac cacttgctcc aggcgccggc gaagggcggc aggccgatga cgctgagcga
     6841 gccgagcagg aacgcgaaca tggtcagcgg catggcgcgg ccgaggccgc gcatgtcgga
     6901 gatgttctcc ttgtgggcgg tgatcaggat cgcgccggcg cagaagaaca gcgtgatctt
     6961 gccgaacgcg tgcatcagca tgtgcaggcc gccgccgacg atcgccgagc tggtggcgag
     7021 cgaggcggca agcacgatgt aggcgagctg gctgaccgtc gagtaggcga gcatggcctt
     7081 caggttgtcc ttggtcaacg cgatggccga ggcggcaagc agggtgaagg tcgcaagcca
     7141 cggcagccac gcgcccgggt tcgcgccctg catcaggtcg aggccgaaca cgtagatcac
     7201 caccttcatc accgtgaaca cgccggcctt gaccaccgcg accgcatgca gcagcgcact
     7261 gaccggcgtc ggcgcgacca tcgccgccgg cagccagcgg tggaacggca tgatcgccgc
     7321 cttgcctatg ccgaacatgt agagcgcgaa caggatcgcg agcacgcccg gctcggcctt
     7381 gccggcgagg atgccgccgg gcctgaagtc gagcgtgccg gcgagcgcgt aggtggcgag
     7441 gatcgcgagc agctggaagc cgatcgaggt gccgagcaag gtgccgagat agagccgccc
     7501 cgaccggcgc gcctcgtcgg tgcccgagtg cgtgaccagc gggtaggtcg acagggtcag
     7561 cagctcgtaa aacaggaaca gggtgaacag gttggcggcg aaggcgaccc cgaccgcgcc
     7621 cgccagcgcc accgcgaagc agacgtagaa ccgggtctgg ttctgctcgt ggtgcgcgcg
     7681 catgtagccg atcgagtaga ccgagttggg aatccacagg aacgaggcga tcagcgcgaa
     7741 cagcatgccg agcggctcgg cgcgaaaggc gagcgcgatc ccgggcagca cgtcgtagag
     7801 ctcggcctcg gggcgcgcgc ccgccatcac cgccggcgcg acccaggcga cggttgcgaa
     7861 cagcgcgccg gcggtcacca gcgtcacggc ctcgcgcagg ttgggcctgg cgccggcgag
     7921 cccgatcatc accgccccga ccagcggcac gaacggcgcg aagccgaccg cgaaggcggc
     7981 gctcacggcg cggtccccag cagcatgccc gccgccaggc gcgcggtgcc gacggtgagc
     8041 tcggtggtga gcccgaacag gatgcaggcg ccggcgagcg cccaggtcgc ggcgaccatc
     8101 ggccacggcg cctcggcgac atcgctcgga ttgtcgggct cggcgaagta ggcggcctcg
     8161 accacccgcc agacatagac gatggccagg atcgaggtgc cgatgatcac caccgccacc
     8221 ggccaccagc cggcctcgag cgcggccagc accaggtacc acttgctgac gaagccgacc
     8281 gtaccgggaa ccccgatcat gctcagcccg gcggcgacga acgccgccat ggtcagcggc
     8341 atgcgcctgc cgagcccttt gaggtgctcg atgcgggtgc cgccgacgcg cagcatgacc
     8401 gcgccgaggg cgaggaacat ggcgcccttc atcagcgcgt ggttgaacag gtggacgacc
     8461 gcgccggtga gcccggtcga ggtggcgaag gcaatgccga tgacgatata gccgatctgg
     8521 gcgaccgagg agtaggcgag cacccgcttg acgttgatct gggtgatcgc gaccagcgag
     8581 cccgacaccg cggcgatcag cgcgagcggc atcaacagga tggtcaccgg gtgcgcgccg
     8641 accgagaagc cgacgccgaa cacgccgaac atgaagcgca ggatcaaata gatgccgacc
     8701 ttggtcgcgg tcgccgcaag cagcgccgag accatcgacg gcgcgtaggt gtaggcgttg
     8761 ggcagccaca ggtgcagcgg gaacagcgcc accttgaggc tgaggccgac gagcaggaag
     8821 gcgtaggccg cgatcaccac caggttgccc tcgagcgccg ggatacgcgc cgccaggtcg
     8881 gccatgttga gggtgccggt ggcgatgtac agcagcccga tcgcgattac gaagaaggtg
     8941 gcgccgatgg tgcccagcac caggtattga tagcttgcga acaatgccct ccggtcgcgg
     9001 ccgaggctca ccagcacgta accagacagc gacgagatct ccatgaacac gaaggcgttg
     9061 aacgcgtcgc ccgtgaccgt catgccgagc aggccggcga aggtgagcag ataggcggtg
     9121 tagaacaggc ggtggcggtc ggcggggatc tcggcggcga cgctgtgata ggcgaacggg
     9181 atcaccgccg cggcgacgcc ggtgacgaac agcgcgacga aggcgttgag gaagtcgacc
     9241 cggtactcga tcccccacgg cgccggccag tcgcccatca tgtagctgat cggcccgtcg
     9301 gccgcgacct tcgccatcag cgcgatcgcc agcgcgaacg cggtccaggt gacgagcagg
     9361 ctgaagcccc aggcgcgcgc gccgcgcccg atcaaggtgc acagcggcgc cccgaacagc
     9421 ggcaccacca cctgcagcgc ggggagattg aggccgatca cgcggcgtcc tcctcgcgcg
     9481 tctcgattgc ctggatctcg tcctcctcga ccgtgccgta ggcctccttg atgcgcacca
     9541 cgagcgcgag gccgagcgcc aaggtcgcca cgccgaccac gatcgcggtc aggatcagca
     9601 cgtgcggcag cgggttcgag tagggcgcgc cgccgtcata gatgatcggc gcggtgccgc
     9661 cgaagatctt gccgctcgag atgtagagca ggaacaccgc ggtctggaag atgttgagcc
     9721 cgatcagctt cttgagcagg ttgccgcggt cgatgacgac gaagaagccg atcatcatca
     9781 ccagcaccac cacccagtag gcgtactcgc cgagcagcgc cacggggcct cagcgctccg
     9841 gctcgccgcg cccggcgaag ctgcagtaga tggcgagcat caccgccgcg accgtcgtgc
     9901 cgacgccgaa ctcgacaatc atgatcccgt agtggtggcc gtgccgggga tcgtgggcga
     9961 gccactggta gtcgaggaaa cgcccgccaa gcagcacggt gagcacgccg gtgccggcgt
    10021 agatgctgag cccgaccgcg accagcacgc gcaccagcgg cggcgggatg gcggcgcgca
    10081 ggttgtcgag gccgaacacc aggccgtaga ggacgcagcc ggcggcgatg atcacgcccg
    10141 cctggaagcc gccgcccggc ccgaagtcgc cgtggaactg aacgtacagg ccgaacagca
    10201 ggatgaacgg gatcaggaac ttggcgacca cgcgcaggat caagagctcg gtcattggtc
    10261 gtccttgccc tcgcccgcgg cgggatcgcg ccggcgccgg ccgagcagca gcatcacgcc
    10321 gactccggcg gtgaagatca ccgccacctc gccgagggtg tcgtagccgc gatagtcggc
    10381 cagcaccgag gtgaccacgt tcgggatgcc gatcgcctcg ggcgtctcgg ccaggtagcg
    10441 cggcaccacg tggtggtgga tcggcgccgc gggatcgccg atcggcggca tgtcctcggt
    10501 ggcatagagc agcacgacac cggtgacgat caccaccgcc agcgcgagca ccggccggcg
    10561 gcgcggaatc ttctcctggt cgccggtcag cgacagcgcg accaggccca gcaccgtggt
    10621 caccccggcg ccgaccgcgg cctcggtgaa ggcgacgtcg actgcatcga gcaccaggaa
    10681 gatgctcgcc atcaggaagc tgtagatgcc gctcagcatc accaccgcga ggatcgagcg
    10741 catgcgcacg atggtcagcg cggtaaccac cagcatggca agcagcgcga tgtggacgaa
    10801 cgcttctatc gctcgccctc cgccgctgca ccgccgccgc gctcgccatc cggcctggtc
    10861 cacggcttga ggccgctgac cagcgccgaa tgggtcagcg cgtggctcgc ggtcgggctg
    10921 gtgaacagca ggaacaggaa gatgaaggcg agccgggcga ggttcaggtc ccagcccgac
    10981 tcgagcatca tgctggcgat catcaggccg gcgccgaggg tgtcggtcat gccggccgcg
    11041 tgcgcccggc tgtagaagtc gggcagccgc accaggccga tgccgccgac catgatgaac
    11101 agcgcgcccg ccagcatgca cgcccaggac aggacgtcgg cgaccaggct catcggcgct
    11161 ggcccgcccg ccggtcgtcg aaccggccga acttgaagta tttcagcgcc gcgatcacgc
    11221 cgaggaagtt gatcagcgca tagaccaggc cgaggtcgag gaactcgggc cgtccggtca
    11281 ggtagccgag caccgcgatc agcagcacgg tcttggtgcc gaacgcgttc gccgccagga
    11341 tgcggtcgaa cacggtcggc ccggcgaccg cgcgcgcgac cgcgagcgcc atggtcgcga
    11401 gcagggcgac ggcggcgacc gcgaactcga ggctcacgcg ccgccctcca acgccgtcac
    11461 cttgcggtcc atggtgcctt cgaggagcgc cgcctcgccc tcggcggcga gcgcgtgcac
    11521 caggatgcgg tcgggggcga ggtcgaccga gatcgtcccc ggggtcagcg tgatcgagtt
    11581 ggcatagatc acccggccga ggtctgtgcg ctggctcgtg cgcacctcga acaggcgcgg
    11641 gtcgatgggc agcttcgggt cgatcacgcg ccgggcgacg tcgatgttgg ccttgacgat
    11701 ctcgaccagc agccacagcc agtagccggg caggagcaag gacatgtgca gcggatggct
    11761 ctcgcggtcg accacgtcga agcggcgtgc gagcgcgcac acaaacgcgc aggacgcggc
    11821 gccgaagccg agcagcagcg gctcgtagtg gccggacagc agaagccaca ccgcggccag
    11881 cgccgcgaac agcgaaacgg tgtgcatcag gcccgccgag tcctcgccgc gtgcgcgcgc
    11941 ccgtcactca ctcgatcacg accgcgtagc tgtccgccgg ccgcgcctcg gcgatcaggc
    12001 cgagatcggc aaggaaccgg ctgaagcgca cgtagcgcag ggcatccagc gcccgcgggc
    12061 ggagcgcgaa gcggggcagc gtgtcgcgcc atgcccgccg gttgagctcg tcgtcgagct
    12121 cgggccggcc gcgcacgaac agcgcccagc tctcgtccgg atggttgacc aggaactgca
    12181 ccccctgctc cagcgccgcc acgaagcgtc ccagtcgcga atcatcgagc cgcgcgcgat
    12241 tggcgaccac gatcagctcg tcataggcgg ggaccccgtg ctcttccaca tagaacgcgc
    12301 gcccgggatg gccggcgagg tccatctggt tgagctcgaa gttgcggaat gcgccgatca
    12361 ccgcgtcgac ctggcccgag atcactgccg gcgacagcga gaagttgaca ttgatcaggg
    12421 taacgtcgtc gagcgcgagg ccgctcgccg tgagcatggc gccgagcagc gcatcctcga
    12481 agccgccgac cgagaacccg accttgcgcc ccttgaggtc ggcgatggtg cggatcgggc
    12541 cgtcggcaag caccaccagg cagttgagcg gcgtcgccac cagggtcgcc acgcgcacca
    12601 gcggcagccc ttcggcaacc tggatgtgaa gctgcggctg gtacgagacc gcgaggtcgg
    12661 ccttgccggc ggcgaccagc ttgggcggat cgttggggtc ggcgggcgcg atcagctcga
    12721 cctccaggcc ttgcgcggcg aagtatccgt tctcctgcgc gaccagcagc ggcgcgtggt
    12781 cggggttgac gaaccagtcg agcaccaagg tcagcttgtc ggccgcgccg gccggacccg
    12841 ccgccgcgat cgagatcgcg accagggccg cgaacgctcg ccccatcctg ctcacccgct
    12901 cacccgcatc gacgggccgg gacgatcgcg tccccgcccc cgaatcggtg gaatcatgcc
    12961 cgcaccggcc gcgccagcac cagcgagaaa tagccgttgg ccggctggta atggaagtcg
    13021 aacgtaaagc ccgccgcgcg cagcatcgcg gccagcgact cgggcgtgaa cttgcggctg
    13081 atctcggtca ggatcgcctc gccggcggcg atatggatgg acgtgccgag cgcgccgagg
    13141 tccacgtcct ggtcgcgccg cgagaccagg tgcatctcga tccggctcag ccggcgatcg
    13201 aaccccgcgc ggtggtcgaa ctggtcgaga tcgaaatcgg cctcggcccc ggcattcagc
    13261 acccgcagga tgttgaggtt gaacgcggcg gtcagcccgg cgcgatcgtt gtaggcggca
    13321 tggaggacgg cctcgtcctt gacccggtcg acgccgagca gaagcaggtc gccgccgttc
    13381 atgcgcgccc tcaggtcgct caggaacgcc accgccttgt cgtgctcgaa gttgccgatg
    13441 gtgctgccga ggaaacagaa cagccgccgc ccgcgcgccg gcggcaggtg ggcaaggccg
    13501 gcgtcgtagt cgccgaccag gacccgcacc cgaagccagg aatactcgtc cgcaagctcg
    13561 cgcgcggcgc gcatcagcgc cgcccgggag atgtcgaacg gcgagtaggt cgggcgcatg
    13621 ccggcggcgc cgcaggcgtc gagcagccgg cgggtcttgc gcgccatgcc gctgccgagc
    13681 tcgaggatat cgtcgggcgc ggcctcggcg atgatggcgg cggcgctcgc atccaggagc
    13741 gcgtcctcgg tgcgggtcag atagtattcg ggcgtgtcgc agatgcggtc gaacagcgcc
    13801 gatccgcgct ggtcgtagaa atatttcggc ggcaggctgc gcggcgccga caacaagccg
    13861 ctgcgcacgt cgtcgagcag gctcgagacg cggcgcgtgg cggccaccgt acggcagtcg
    13921 atccggtcgt ccacatcggg gacgggcgat tggtcgggca tgtcgtcccc ttttcccagc
    13981 cggcctcgca cccggtcgcg gcggaggctg ttacgcgagg cgcgggtaag ctagcatttc
    14041 gtgatcgcga ggcaaccctg cgcgcacctc accgacagac gaattcgccg atgtgccgac
    14101 acgccgccta ccttggaccg ccgatcgcgc tcaaccgatt cctcgccgag ccgtcgcaca
    14161 gcctgatggt gcaatcgtgg cggccgcgcg agcagatcac cgcgaccgtg aacgccgacg
    14221 gcttcggctt cggctggtat ctcgacgacg gccgggccgg cgtctacgcc aacccgatgc
    14281 cgatctggtc ggaccccaat ctcgaccacc tcggcgcggc gttgtcgagc cggctgtgga
    14341 tcggcaacgt gcgcagcgcc acgcgcggcc agccggtcgc ccaggtcaac acccatccgt
    14401 tcctggccgg gcgcgagctg ttctcgcaca acggcttcgt tcacgatttc gcgaacagcc
    14461 tgcgcggcac cttgcgccgc catctcgagc ccgcgttcga ggcgcagatt cgcggcagca
    14521 ccgattccga gtacctgttc gcggcgctgc gccaggagac ggccgcgggc ggcggcaccg
    14581 cggcggcgct gcgccggctg ttcgcgcggc tggaggcgtg gctcgccggc agccacggcc
    14641 tgttcaacct gctgatcgcc gacggcgagc gcatctacgc gacccgccac gcgctgagtc
    14701 acgcctcgcc gtcgctctat gtcggcggcg atgccgagct gttcgacggc ggctggctgg
    14761 tcgcctccga gccgctgacc ggcggcggcg gctggcagga ggtgccggac caccatctgg
    14821 tggtgatcga ggccgggcgc gagccccggc tggagccgct gtgagcggcg ccgcgcgtgc
    14881 ccgcctcagg gcgttgcagg cggtgctcca ggcgaccgtc gccgactgtg gcgaggccga
    14941 tgcgcgcacc cagttcgatc ccgcgctcag cccgctcggc tggcacctgc gccactgcgc
    15001 gttcgtcgag gcgctgtgga ttcgcgagcg ggtgctgggc gacgaccggc tgaccgcgcc
    15061 gctcgccgag gactgcctgc ccgagcgggt gccgaaggag gcgcgcggcg agtccctgcc
    15121 cgggcatcag gcgctgctcg actggaccgc gacgaccatg gccgacaacc tcgagcttct
    15181 gggcgacgcc gagcgcaggc gcgagcgcca cgggctgctc gccgacggct acctgtggga
    15241 cttcctgatc ggccaccacg cccagcatct ggagaccatg cgcatggtgc gcgcggcgcg
    15301 cgccgtggcg ggggcgggcg gcgggaccac cggcggcgca gtgcgcgcgg ctgtgcccga
    15361 ctgggagtgg cgccggctgc cggcggggcg ctacgagatc ggcaccgagg acggcttcgg
    15421 cttcgacaac gaaggcaccg cgcagatggt ggcgctggcc ggcgtgctga tcgcggcgac
    15481 gccggtgagc aacgcgcagt atctcgcctt catcgaggac ggcggctacg acgagaagcg
    15541 ccggtggtcc gcggccggct ggcgctggcg cacatccagc ggggtgacct gcccgcatgg
    15601 ctggcggcgg ggcgcggccg gcggctggct cgaggtcggg cccgacgggg cgcaggcgct
    15661 cacgcccggg gcggcggtgg tcgggctcgg ccgccatgaa gccggcgcgt tcgccgcctg
    15721 ggccggggcg gcgctgccgc acgagtacca gtgggaggcc gccgcccgcc tcgggctcct
    15781 ggaacggacc ggcgaggcct gggagtggtg cgccaacgcc ttccacccct atcccggatt
    15841 ccgcgcccat ccctaccgcg agtactcggc gccgtggttc gacggccgcc acggggtgct
    15901 gcgcggcggc agccgctaca gcgagcccga aatgcggcgc cccggcttcc gcaactatta
    15961 cgagccggcc tgccggcacg tgttcgccgg cctgcggctc gcccgcccgg cctagtcggg
    16021 accgggaccg gtctcgggca gccacggcag cgccgcgcgg gcgagcttat cgaccgcgaa
    16081 gtagatcgcg atcgcggcca ccgccaaggt gaccagcgcc gcgaacatca ggtcgatctg
    16141 catgcggccg ttggcgtgca gcatcaggta gccgaggccg gcgctcgagc ccacccactc
    16201 gccgaccacg gcgccgatcg gcgccaccgc cgcggcgacc ttggcgcccg aggcgagcgc
    16261 cggcagcgcc gccggcacgc ggatgcggac cagcgccgag aaacgcgacg cgcccatggt
    16321 cgcagcgagg tcgagccagc ccgcatccgt gcgcctgagc ccgtcgtaga aggtggcggt
    16381 gaccgggaag tagatgatca gcaccgccat cgccaccttc gaggcgagcc cgtagccgag
    16441 ccacagtacc agcaccggcg cgagcgcaaa gacgggcagc gcctggctga ccacgagcac
    16501 tggcagcagc cagccgcgcg cggtgcggaa gtgcgccagc accagcgcgc tcagcacccc
    16561 gagcgcggcg ccgatcagca gcccgagcag gatctcgatc acggtggtgc cggcatggtc
    16621 gagcagggtc gcgcgatgaa tccaccaggc gctcgccacc cgccccggcc cgggcaggat
    16681 gtagggcggc gtggccgcga gccacaccag cgcctgccag gcggcggcga gcagggcggc
    16741 gatggcgagg cccctgagcg cggtcacggc ctcagctcga tggtgtcggc ggcggtcagc
    16801 cggcgcatca ggtcgccgtg cagggcggcg atgtcgggcg cgccgatcgc gcgcggcggc
    16861 ggccccggcg gcaccagcga ttcgccgacc gtcgccgggc gcccggccat caccaggatg
    16921 cggtcggcga tgcgcagcgc ctcgagcggg tcgtgggtga ccaagagcac cgtgcgcccg
    16981 cgcagggtcg cgaccgcgag gtcctggagg cgggtgcggg tgatcgcatc gagcgccgag
    17041 aacggctcgt ccatgagcac gatcgggcgg tcctccagca gcgtgcgggc gagcgcgatg
    17101 cgctggcgca tgccgcccga cagcgccgcc gggcgggcgc gctcatgccc ggcgaggccg
    17161 acgcgggcga tcagcgcccg cgcctgcgcg accggcgccc gctcgccgcg cagccgcggc
    17221 ccgagcatga cgttctccag cgccgtcagc cacggcagca gcaggtcgga ctgggccatg
    17281 taggcgaccc ggcccgccgc cggccggccg tcgtcgcacc acacccggcc ctcctcgccg
    17341 gccggcgcga ggccggcgat caggcgcagc aggctggtct tgccgacccc gctcgggccg
    17401 agcaggcagg tcagccgccc ggcctcgagc gtcacggcca ggtcctcgaa cagcaccgtg
//
LOCUS       DASWHU010000262         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1247096, whole genome shotgun sequence.
ACCESSION   DASWHU010000262 DASWHU010000000
VERSION     DASWHU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247096"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..965
                     /locus_tag="VGB88_09695"
     CDS             222..965
                     /locus_tag="VGB88_09695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647760.1"
                     /translation="MADSDYRTALVTGASSGIGEAVVRALCARGLGVTALARRAQRLE
                     QLAQATGCEAVVVDVRDRDALYAALDDREVDILVNNAGIGRGMTGLAETDPDDIDAAI
                     DTNVGAVLHAVRALVPGMIARGRGHIVNIGSIAGMYPIHSALYGGSKGAVRLLSQNLR
                     LELLGTGVRVTEICPGRVMTEFFEQAYGDAEVVERITSGFDLLQPADIAEAIVYALEA
                     PWHVNVATIELLPTEQAPGGVQSRPIERS"
     gene            973..2706
                     /gene="araD"
                     /locus_tag="VGB88_09700"
     CDS             973..2706
                     /gene="araD"
                     /locus_tag="VGB88_09700"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX9647761.1"
                     /translation="MPQTRNPERLRSARWFAPDDLRSAGHRSRIRQLGFGPDDFAGRP
                     VIAILNTWSELNTCHGHFRQRAEEVKRGVFQAGGFPVELPALSLGEIMMKPTTMLYRN
                     LLAMEAEELLRCHPVDGAVLMGGCDKTTPALLMAAASMNIPAIYLPAGPMLRGYWRGR
                     NLGSGTDMWKYWAELRAGNITDEDWSEIEAGMGRSHGTCMTMGTASTMTSLADALGMT
                     LPGASSIPAADSGHARMAAACGRRIVDMVWEDLKPSDIMTERAFANALRVDMALGGST
                     NAAIHLVAIAGRLGVELSLDRFDEAAREVPLLANISPSGAYLMEDFYYAGGLRALLAR
                     LSDRLDLGAPCANGRSLGDNIAGAASHNDDVIRTLNDPVSTEPTTAVLRGNLAPDGCV
                     IKPIAADRALLKHTGPAVVFRDYNDMAARIDADDLPATPDSVLVLQNGGPLGGPGMPE
                     WGMLPIPQKLLKQGVRDMVRISDARMSGTSYGTCVLHVAPESFVGGPLALARDGDLIT
                     LDVPARTLTLNVDDAELGRRRAAWRPPAPRYGRGYGAMYASHVTQADKGCDFDFLRRD
                     AGAPVPEPEIH"
     gene            2706..>3297
                     /locus_tag="VGB88_09705"
     CDS             2706..>3297
                     /locus_tag="VGB88_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEX9647762.1"
                     /translation="MAGPDNPFKRAIAEGRPQVGLWASLASNVVCNVLADSGFDWIVI
                     DTEHAPNDLTTVLGQLQALGGGPATPFVRPAWNDQVLIKRLMDIGAHSLIVPFVQSRE
                     QAREAVRFTRYPPDGVRGVAVATRANRYGRDASYFRTVNDRVCVIAQIETRSALDQLE
                     DIASVDGIDGIFIGPSDLSADLGHLGDPGSAATQELI"
BASE COUNT          473 a         1215 c         1149 g          460 t
ORIGIN      
        1 gacgttggcg ctgcttccgg tcgcggttgc cgccctacac ttcggtgtca tcccgcgcga
       61 ggaagcgcat ctcgcccgcc gcttcgccga cgcctatcgc gactaccgcg gacgggtccg
      121 gcgctggctg tgaccgcggg ggagcgcggt ttgccgcacc tgccgctttc ggccatactc
      181 gcggcgcgcc ggcgcgcccg tgccggcggc ggggaggacg catggcggat tcggactacc
      241 ggacggcgct ggtcaccggc gcctccagcg gcatcggcga ggcggtggtg cgcgcgctgt
      301 gtgcgcgcgg cctcggcgtg accgcgctcg cgcgccgcgc gcaacggctc gagcagctgg
      361 cccaggccac cggctgcgag gccgtggtgg tcgacgtgcg cgaccgcgac gcgctctacg
      421 ccgccctcga cgaccgcgag gtcgacatct tggtcaacaa tgccggcatc gggcgcggca
      481 tgaccggcct cgccgagacc gacccggacg acatcgacgc cgccatcgac accaatgtcg
      541 gcgcggtgct ccatgcggtg cgcgcgctgg ttccgggcat gatcgcgcgc gggcgcgggc
      601 acatcgtcaa tatcggctcg atcgccggca tgtacccgat ccactcggcg ctctacggcg
      661 gctcgaaggg cgcggtgcgg ctgctgtcgc agaacctccg cctcgagctc ttgggcaccg
      721 gagtgcgggt caccgagatc tgcccggggc gggtgatgac cgagtttttc gagcaggcat
      781 acggcgatgc cgaggtggtg gagcggatca ccagcggctt cgacctgctc caacccgccg
      841 acatcgccga ggcgatcgtc tacgcgctgg aggcgccgtg gcacgtgaat gttgccacca
      901 tcgagctgtt gccgaccgag caggcgccgg gcggcgtcca gtcgcgcccg atcgagagga
      961 gctgagccgg cgatgccaca gacgcgcaat ccggagcgcc tgcgcagcgc acgctggttc
     1021 gcgcccgacg acctgcgctc ggccgggcac cgctcgcgca tccgccagct cggcttcggc
     1081 cccgacgact tcgccggccg cccggtgatc gcgatcctca atacctggag cgagctcaac
     1141 acctgccacg gccacttccg ccagcgcgcc gaggaggtca agcgcggcgt gttccaggcc
     1201 ggcggcttcc cggtcgagct gccggcgctg tcgctcggcg agatcatgat gaagccgacc
     1261 accatgctct accgcaatct cctggcgatg gaggcggaag agctgttgcg ttgccacccg
     1321 gtcgacggcg cggtgctgat gggcggctgc gacaagacca cgcccgcgct gttgatggcc
     1381 gcggcaagca tgaacattcc cgcgatctat ctgcccgcag ggcccatgct gcgcggctac
     1441 tggcgcggcc ggaacctcgg ctcgggcacc gacatgtgga agtactgggc ggagctgcgc
     1501 gcgggcaaca tcaccgacga ggactggtcg gagatcgagg ccggcatggg gcgcagccac
     1561 ggcacctgca tgaccatggg caccgcctcg accatgacct cgctcgccga tgcgctcggc
     1621 atgaccttgc ccggcgcaag ctcgatcccg gccgccgact cgggccatgc gcgcatggcc
     1681 gcggcgtgcg ggcggcggat cgtcgacatg gtgtgggagg acctcaagcc gagcgacatc
     1741 atgaccgagc gcgcgttcgc caacgcgttg cgggtcgaca tggcgctcgg cggctcgacg
     1801 aatgccgcca tccacctggt cgcgatcgcg ggccggctcg gcgtcgagct gtccctcgac
     1861 cgcttcgacg aggccgcgcg cgaggtgccg ctgttggcca acatcagccc atcgggcgca
     1921 tacctgatgg aggacttcta ctatgccggc ggcctgcgcg cgctgctcgc gcgcctgtcg
     1981 gaccgcctcg acctcggcgc gccgtgcgcc aacgggcgca gcctcggcga caacatcgcc
     2041 ggcgccgcga gccacaacga cgacgtcatc cgcaccctca acgacccggt ctcgaccgag
     2101 ccgaccaccg ccgtgctgcg cggcaacctc gcgcccgacg gctgcgtgat caagccgatc
     2161 gccgccgacc gtgcgctgct caagcacacc ggcccggcgg tcgtgttccg cgactacaac
     2221 gacatggccg cgcgcatcga cgccgacgac cttccggcca ccccggactc ggtgctggtg
     2281 ttgcagaacg gcggcccgct gggcgggccg ggcatgcccg aatggggcat gctgccgatc
     2341 ccgcaaaagc tgctgaagca gggcgtgcgc gacatggtgc gcatctcgga cgcgcgcatg
     2401 agcggcacct cctatggcac ctgcgtgctg cacgtcgccc cggaatcgtt cgtcggcggt
     2461 ccgctggcgc tggcgcgcga cggcgacttg atcacgctcg atgtgccggc gcgcacgctc
     2521 accctgaacg tggacgatgc cgagctcgga cgccggcgcg cggcatggcg gccgccggcg
     2581 ccacgctacg gccgcggcta cggcgccatg tacgcgagcc acgtcaccca ggccgacaag
     2641 ggctgcgact tcgacttcct gcgccgcgac gccggcgcgc cggtgccgga gccggagatc
     2701 cactgatggc cgggcccgac aacccgttca agcgcgcgat cgccgagggc cggccgcagg
     2761 tcgggctgtg ggcgagcctg gcgagcaacg tggtgtgcaa cgtgctcgcc gactccggct
     2821 tcgactggat cgtgatcgac accgagcacg cgcccaacga cctcaccacc gtgctcggcc
     2881 agctccaggc gctcggcggc ggcccggcga cccccttcgt gcgcccggcg tggaacgacc
     2941 aggtgctgat caagcggctg atggacatcg gcgcgcactc gctgatcgtg cccttcgtcc
     3001 agagccgcga gcaggcgcgc gaggcggtgc ggttcacccg ctatccgccc gacggcgtgc
     3061 gcggggtcgc ggtcgccacc cgcgccaacc gctatggccg cgacgcaagc tacttccgca
     3121 ccgtcaacga ccgggtgtgc gtgatcgccc agatcgagac ccgctccgcg ctcgaccagc
     3181 tcgaggacat cgcctcggtc gacggcatcg acggcatctt catcggtccc tcggacctgt
     3241 cggccgacct cggccacctc ggcgacccgg gcagcgcggc cacccaggag ctgatcg
//
LOCUS       DASWHU010000263         5476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1248571, whole genome shotgun sequence.
ACCESSION   DASWHU010000263 DASWHU010000000
VERSION     DASWHU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248571"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1647
                     /locus_tag="VGB88_09710"
     CDS             130..1647
                     /locus_tag="VGB88_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX9647763.1"
                     /translation="MSETGSDQDGAAEEPGPGRARTVGLVAGPAAAAAMLLAGPPAGL
                     GAPAWQCAAVAVLMALWWMTEALPIPATALIPLAAFPLLGVASTAAAAAPYAHPLVFL
                     FMGGFLIALAMQRWELHRRIALNLVALFGTRPARLIAGFMVATAALSMWVSNTATTLM
                     MLPIAISVIALLRRDAGAVLDRNFPAALLLGIAYAASIGGIGTLIGTPPNAFLAGFMQ
                     ESYGVTIGFAEWLLVGLPLVAVMLPLAWLTLVRVACPIRLKEAGAAAAVIRGELAALG
                     PMGRGERTVLVVFAATAAAWIFRPLISETMPGLGLDDTVIAIAGALVLFVIPVNWKER
                     TFALDWRWAIKLPWGVLLLFGGGLTLADAIRTTGLADWIGGGTGALGGLPVVVIVIAV
                     TALIILLTELTSNTATTATFLPIVASVAVGLGENPLLLAVPAALAASCAFMMPVATPP
                     NAIVFASGHLRIGQMIRAGIWLNLIGVAAITALGYTVVLAVLGVEPGTLPAWAAP"
     gene            complement(1659..3440)
                     /locus_tag="VGB88_09715"
     CDS             complement(1659..3440)
                     /locus_tag="VGB88_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9647764.1"
                     /translation="MRLDSWANLAAMFFEQAAARGGAPFLWHKQDGAYRAISWGEAAR
                     QASALARALRAYGLTPGERVLLLSENSPQWAIADFAIMAAGGITVPAYTTNTVRDHAH
                     VLTDSGAAMAVVSTAALAGPLLAAAAGAPALRRVITIEPVPAPAGRPLVSGWDEALAE
                     GAAAPGDPAADAARIARDSLACLIYTSGTGGVPKGVMLSHRAIMANCVGAYHLLLELG
                     LGDEVFLSFLPLSHSYEHSCGLMFPVSLGAQIYYAERVETLSANLLEARPTIMTAVPR
                     LYEVMRERILHGVRRQGGLKARLFRAAVELGRKRYEQPGALSLGERLIDAVVERLVRD
                     KVRARFGGRLKALVSGGAPLNYEVGVFFTALGLRLLQGYGQTEAAPVVSCNPPSRIKL
                     RTVGPPLADVEVRIADDGEILVRGPLLMDGYWNQPELSAEVLRDGWLHTGDIGVIDQD
                     GCIQITDRKKDIIVNSGGDNLSPQRVEGILALEPEIETAMVVGDRRPHLVGLIVPAQA
                     FVEQWAKDHNTRADLAALSGDRSFRNALGAAVDRANAGLSVIEKVKRFIVAKEPLTVE
                     NGSMTPTMKLRRHVILERYGAALDELY"
     gene            complement(3565..4188)
                     /locus_tag="VGB88_09720"
     CDS             complement(3565..4188)
                     /locus_tag="VGB88_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647765.1"
                     /translation="MNGKALVTATAAAAVALTIGIAGALAEPPERMQNRQMQAGRFDD
                     GGVGVRKSYATSEGRAWAGDGQSGAQGTATAGAFTAGRRADGSTGVRKAFSATNILTS
                     GQVGGGVSTTNASGRTTAQSFMAGPGGARRTSITSTLGTSAQVTGDGTAASVVGAGST
                     AVAGDAMATTLATGGSVASGGGAASAVSVGAAASAGGLSAGPGRKRR"
     gene            complement(4219..4626)
                     /locus_tag="VGB88_09725"
     CDS             complement(4219..4626)
                     /locus_tag="VGB88_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647766.1"
                     /translation="MQARLSSIAAIAAAAALAAGGALGHESSVTVGSSAGQALFASTA
                     TSGSSWWRILDDDDGSVVVIERDGHAQRYICPPGRTVTVMQTTGVGTSAGGISSSITL
                     GGGHGSAVTTIGGNGVSVGVGPGGESHVTVTCH"
     gene            complement(4714..5271)
                     /locus_tag="VGB88_09730"
     CDS             complement(4714..5271)
                     /locus_tag="VGB88_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647767.1"
                     /translation="MQRPASQALESVIRRHVADHGRRIIAKGNRKMTSRIEIGHKQSR
                     VAKTARPALAALAAALLLGTAAGASAQTVGTTVATGGTSVGGGMIGTTIGTGGSYAVA
                     GGSGTTIGTGGTVIQGGQLGTALSLGGSFVGSGRGGGSTGTTLGTGGTFIQDGQLGTS
                     LATGGSFAGSDGDRPNRRKRSHRHR"
BASE COUNT          662 a         2049 c         1924 g          841 t
ORIGIN      
        1 ggcgagcgtg atcatcggcg gcatggcgtc gtcgacattg ctcaacctcc tggtgctgcc
       61 ctcgatcatg taccgctacg gccgcttcgg agcgcgcacc gggctggtcg gcgaggccgc
      121 ggcggcgcga tgagcgagac gggatcggac caggacggcg ccgccgagga gcccggcccg
      181 ggccgcgcgc gcaccgtcgg cctggtcgcc ggccccgcgg cagcggcggc gatgctgctc
      241 gccggcccgc ctgcggggct cggcgcgccg gcctggcaat gcgccgcggt cgcggtgctg
      301 atggcgctgt ggtggatgac cgaggcgctg ccgatcccgg cgaccgcgct cattccgctc
      361 gccgccttcc cgctgctcgg cgtcgcctcc accgccgccg ccgccgctcc ttacgcccac
      421 cccctggtgt tcctgttcat gggcggcttc ctgatcgcgc tcgccatgca gcgctgggag
      481 ctgcaccggc gcatcgcgct caacctggta gcgctgttcg gcaccaggcc cgcgcgcctt
      541 atcgccggct tcatggtcgc gaccgcggcg ctcagcatgt gggtgagcaa caccgcgacc
      601 accttgatga tgctgccgat cgcgatctcc gtgatcgcgc tgctgcgccg ggacgccggc
      661 gccgtgctcg accgcaactt ccccgccgcg ctgctgctcg gcatcgccta cgccgccagc
      721 atcggcggca tcggcacctt gatcggcacg ccgccgaacg cgttcctggc gggcttcatg
      781 caggagtcct acggcgtcac catcggcttc gccgaatggc tgctggtcgg cctgccgctg
      841 gtcgcggtga tgctgccgct ggcctggctg accctggtgc gcgtggcctg cccgatcagg
      901 ctcaaggagg ccggcgcggc ggcggcggtg atccggggcg agcttgccgc gctggggccg
      961 atgggccgcg gcgagcgcac cgtgctggtg gtgttcgccg cgaccgcggc ggcatggatc
     1021 ttccggccgc tgatctcaga gacgatgccg ggactcggcc tcgacgacac ggtcatcgcg
     1081 atcgccggcg cgctggtgct gttcgtgatc ccggtcaact ggaaagagcg cacattcgcc
     1141 ctcgactggc ggtgggcgat caagctgccg tggggcgtgc tgctgctgtt cggcggcggg
     1201 ctcacgctcg ccgatgcgat caggaccacc gggctcgccg actggatcgg cggcggcacg
     1261 ggcgcgctcg gcggcctgcc ggtagtcgtc atcgtcatcg cggtcaccgc gctgatcatc
     1321 ctgctgaccg agctgaccag caacaccgcg accaccgcga ccttcttgcc gatcgtcgcc
     1381 tcggtcgcgg tgggcttggg cgagaacccg ctgctgctgg cggtgcccgc ggcgctggcg
     1441 gcgagctgtg cgttcatgat gccggtcgcc accccgccga acgcgatcgt gttcgcgagc
     1501 gggcatctgc gcatcggcca gatgatccgc gccggcatct ggctcaacct gatcggcgtc
     1561 gcggcgatca ccgcgctcgg ctacacggtg gtcctcgccg tcctcggcgt cgagcccggc
     1621 accctccccg cctgggccgc gccgtagacc ttgccggttc agtacagctc gtcgagcgcg
     1681 gcgccgtagc gttccaggat gacgtggcgc cgcagcttca tggtcggagt catcgacccg
     1741 ttctcgaccg tgagcggctc cttggcgacg atgaagcgct tgaccttctc gatcaccgac
     1801 aggccggcat tggcgcggtc caccgccgcg ccgagcgcgt tgcggaagct ccggtcgccg
     1861 gacaaggccg ccaagtcggc gcgggtgttg tggtccttcg cccattgctc gacgaacgct
     1921 tgcgcgggaa cgatcagccc gaccagatgc gggcgccggt cgccgaccac catcgcggtc
     1981 tcgatctcgg gctcgagcgc gaggatccct tcaacgcgct gcggcgacag gttgtcgccg
     2041 cccgagttga cgatgatgtc cttcttgcgg tcggtgatct ggatgcagcc gtcctggtcg
     2101 atgacgccga tgtcgccggt gtgcagccag ccgtcgcgca gcacctccgc cgacagctcg
     2161 ggctggttcc agtagccgtc catcagcagc ggcccgcgca ccaggatctc gccgtcgtcg
     2221 gcgatcctga cctcgacgtc ggcgagcggc gggccgaccg tgcgcagctt gatccggctc
     2281 ggcgggttgc agctcaccac cggcgccgcc tcggtctggc cgtagccctg cagcaatctc
     2341 aagcccagcg cggtgaagaa cacgccgacc tcgtagttga gcggcgcgcc gcccgagacc
     2401 aacgccttca gccggccgcc gaagcgcgcg cgcaccttgt cgcgcaccag ccgctcgacc
     2461 accgcgtcga tcaggcgctc gccgaggctg agcgcgcccg gctgctcgta gcgcttgcgc
     2521 ccgagctcga ccgcggcccg gaacagccgc gccttgaggc cgccctggcg gcgcacgccg
     2581 tgcaggatgc gctcgcgcat cacctcgtac agcctgggga ccgcggtcat gatggtgggc
     2641 cgtgcctcga gcaggttcgc gctcaaggtc tcgacccgtt cggcgtagta gatctgggcg
     2701 ccgagcgaga ccgggaacat caggccgcag gaatgctcgt aggagtgcga cagcggcagg
     2761 aacgacagga acacctcgtc gccgaggccg agctcgagca gcaggtgata ggcgccgacg
     2821 cagttggcca tgatcgcgcg gtggctgagc atcaccccct tgggcacgcc gccggtgccg
     2881 gaggtgtaga tcaggcaggc gaggctgtcg cgcgcgatgc gcgcggcgtc ggcggcagga
     2941 tcgccgggcg cagcggcgcc ctcggcgagc gcctcgtccc agcccgagac cagcggccgg
     3001 ccggcgggcg ccggcaccgg ctcgatcgtg atcacgcgcc tgagcgcagg cgcgcccgcg
     3061 gccgcagcca gcagcggccc ggccagcgcc gcggtcgaga ccaccgccat cgccgcgccg
     3121 gaatcggtca gcacgtgggc gtggtcgcgc acggtgttgg tggtgtaggc cggcaccgtg
     3181 atgccgcccg cggccatgat cgcgaaatcc gcgatcgccc actgcggcga gttctccgac
     3241 agcagcagca cccgctcgcc cggcgtgagc ccataggccc tgagcgcgcg cgcgagcgcg
     3301 ctcgcctggc gcgcggcctc gccccaactg atcgcccgat acgcgccgtc ctgcttgtgc
     3361 cacaggaacg gcgccccgcc ccgcgcggcg gcctgctcga agaacatcgc cgcgaggttg
     3421 gcccagctgt cgagcctcat cgctcccccc cgttcgcgct cctccggggc cgatcttagc
     3481 ccggattcgc cgccctggcg cgcgctgtcg tcgcgccggg gcgctcggcg cgcgaggcga
     3541 cgggggggag cggcggagcg acggctacct gcgcttccgc cccgggcccg ccgacaggcc
     3601 gcccgcgctc gcggcagcac cgacgctgac cgcgctggcc gcgccgccgc cgctcgcgac
     3661 ggagccgccg gtggcgaggg tcgtggccat ggcgtcgccc gcgaccgcgg tcgagccggc
     3721 gcccacgaca cttgccgccg tgccgtcgcc ggtcacctgc gccgaggtgc cgagcgtact
     3781 ggtgatgctc gtgcgcctgg caccccccgg cccggccatg aagctctgcg cggtggtccg
     3841 tcccgacgcg ttggtcgtgc tgacgccgcc gccgacctgg cccgaggtga ggatgttggt
     3901 cgcgctgaac gccttgcgca cgccggtcga gccgtcggcg cggcgcccgg cggtgaaggc
     3961 gccggccgtc gccgtgccct gcgccccgga ctgcccgtcg ccggcccacg cccggccctc
     4021 gctcgtcgca tagctcttgc gcacgccgac gccgccgtcg tcgaaccgcc cggcttgcat
     4081 ctggcggttc tgcatgcgct cgggcggctc tgcgagcgcg ccggcgatgc cgatcgtcag
     4141 cgccaccgcc gccgccgcgg ttgcggtgac caatgccttg ccgttcatgg ctgtcgctcc
     4201 ccttgcccga gcctggtctc aatggcacgt gaccgtgaca tggctctcgc cgcccggccc
     4261 gacgccaacg ctgacgccgt tgccgccgat ggtggtgacg gcgctgccgt ggccgccgcc
     4321 gagggtgatc gagctcgaga tgccgcccgc ggaggtgccg actccggtcg tctgcatgac
     4381 ggtgaccgtg cgcccgggcg ggcagatata cctctgcgcg tggccgtcgc gttcgatcac
     4441 gaccacggag ccgtcatcgt cgtcgagaat ccgccaccag ctgctgccgc tggtcgccgt
     4501 gctcgcgaag agcgcctggc cggcactgct tccgaccgtc accgaggact cgtgcccgag
     4561 cgcgccacct gccgccaggg ctgccgccgc ggcgatcgcc gcgattgaac tcaaccttgc
     4621 ttgcatctga cttctcctgc ctcaccaagt ggggagcggg agcggcgccg ccggagccgg
     4681 tcggcaccgc tcccccctgt gcccgtcgtc ggactagcgg tgacgatgcg accgcttgcg
     4741 cctgttcggc cggtccccgt ccgagccggc gaagctgccg ccggtggcga ggctggtgcc
     4801 cagctgcccg tcctggatga acgtgccgcc cgtgccgagc gtggtgccgg tgctgccgcc
     4861 gccgcggccg gagccgacga agctcccgcc gagggacagc gccgtgccca gctgaccgcc
     4921 ctggatgacg gtgccgccgg tgccgatcgt ggtgccgctg ccgccggcga cggcgtagct
     4981 gccgccggtg ccgatggtgg tcccgatcat gccgccgccg accgaggtgc cgccggtggc
     5041 gacggtggtg cccaccgtct gcgccgaggc gcccgccgcc gtacccagca gcaacgccgc
     5101 cgcgagcgcg gcaagcgccg gccgcgcggt cttcgcgaca cgagactgct tgtgcccaat
     5161 ttcgattcga ctggtcatct tccggtttcc tttcgcaatg atccgcctgc cgtgatccgc
     5221 cacatggcgg cggatcacgc tttcgagcgc ctggcttgcc ggtcgttgca tcggcaaccg
     5281 gtgctcgaca cgacgccacc atagaaaagg caagctgaac cggcgatgaa ccgctcccgc
     5341 cggttttatt tttttgccgc ttcactccgg tggccggtct tcgggctcct ctcctggtcc
     5401 cgaagggcgc ggccgcgccg cgccgatcac gctcggtctc ggcgtcttct ccgaaggcgc
     5461 gacggtatag attcta
//
LOCUS       DASWHU010000264        11053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1249592, whole genome shotgun sequence.
ACCESSION   DASWHU010000264 DASWHU010000000
VERSION     DASWHU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249592"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGB88_09735"
     CDS             complement(<1..180)
                     /locus_tag="VGB88_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9647768.1"
                     /translation="MTETGTSALVGTDWLANHLGDPDLRVVDATYHLSDLGRDAEAEY
                     QACHIPGAVFLDIDRV"
     gene            complement(190..1041)
                     /gene="sseA"
                     /locus_tag="VGB88_09740"
     CDS             complement(190..1041)
                     /gene="sseA"
                     /locus_tag="VGB88_09740"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX9647769.1"
                     /translation="MDYVNPEALVSTRWLADHLDSPGLRVVDGSWHMPQLKRDARAEF
                     EAGHIPGAVYFDIDRIADRADPLPHMLPDAATFARDVGALGIANDAHVVAYDTVGVGS
                     AARVWWTFRAFGHENVSVLDGGLPKWLAEGRPVARGPATPRAATYKARLDAALVRGLA
                     EVRAAVANGSEQILDARSRGRFAGTEPEPRAGLRGGHMPGALSQPYLELYEGEAKTMK
                     QAADLRALFKDAGLDLSRPVVTSCGSGVTACNLALALHLIGKPDVAVYDGSWTEWGGR
                     DDTPIET"
     gene            complement(1043..1765)
                     /locus_tag="VGB88_09745"
     CDS             complement(1043..1765)
                     /locus_tag="VGB88_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX9647770.1"
                     /translation="MSELLFRDDAYLTECAARVVAADERGVRLDRTVFYARGGGQPGD
                     SGVLETDSGVRVEIVDAVKGEAADEVIHVPADGAPPLAPGTAVSASIDWPRRHRLMRM
                     HSCLHLLSALVPGAVTGGQVGADRSRLDFDLPEGGLDKDALSAALNRLIAEDHPLVAR
                     WVSEAELDANPDLVKTMSVKPPRSAGSVRLIEVAGVDVQACGGTHVRRSGEIGPVRVI
                     KIESKGRRNRRVTVALAAKEDG"
     gene            complement(1758..2780)
                     /locus_tag="VGB88_09750"
     CDS             complement(1758..2780)
                     /locus_tag="VGB88_09750"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX9647771.1"
                     /translation="MEVKEGFVDLIGNTPLIRLARASRETGCEILGKCEFLNPGQSVK
                     DRAALFIVRDAEARGTLRKGGVIVEGTAGNTGIGLALVGNALGYRTVIVIPETQSQEK
                     IDMLRMCGADLRLVPAVPYKDPNNYVKVSGRIAAELNQAGPEGAIWANQFDNVANRQA
                     HVEGTGPEIWRQTDGRIDGFTCAVGTGGTLAGVAMALKERDRDVQVWASDPLGAQVYS
                     LLKPGAPVIKGDSITEGIGQGRITANLEGAPIDDAQQVTDTEALEVVFALAKEEGLVL
                     GGSSGINVAGAVKMAKAMGRGHTIVTILADYGNRYASKMFNPAFLRSKDLPVPDWLDK
                     EPVRGV"
     gene            complement(2812..3645)
                     /locus_tag="VGB88_09755"
     CDS             complement(2812..3645)
                     /locus_tag="VGB88_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647772.1"
                     /translation="MQASHALAAVGALSRRELIKFVQQKGRLFSAFVRPALWLFVFAV
                     GFRGVYGVDAGEPYLARNIEYEYYILPGLTCMVLLFSGMQSSLSMVYDREMGVMRLLL
                     TAPLPRWYLLTAKLVSGTFLSVLQAYAFLVICLPFDWLTSLHVAFNDPLAWLYVLPAL
                     IMTGMMLGALGLLLSVHIKQLENFAGTMNFVIFPMFFISPALFPLWYLQESGADIVYW
                     VANANPFTYGVQLVRYASEGQLYGLGAVVVCATTLVFLIAAIIGYDPQRGFVRRGPQA
                     A"
     gene            complement(3682..4896)
                     /locus_tag="VGB88_09760"
     CDS             complement(3682..4896)
                     /locus_tag="VGB88_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647773.1"
                     /translation="MFRPFVAALLLLAIVAAAPVPVPAAEPDQNELPTLTIGYVDLEP
                     DVRYDDWGLHPVDIRSATAIVDRHAWAGAELGLADVAQLKRVAKANFALERRTVADAA
                     AAIEAIKGLAADGVSLFVLDLPDAAVAEVAKATRDSGVLLFNATATGDSLRNDDCAPH
                     LFHTAASRAMLMDGLAQFLKAKKWTRVLILQGPLAEDALTASAFKRAAKLFGVAVTET
                     RSFVLGSDPRARELNDLAFLTGNASYDAVMVADADGEFALAVPYDTLQPAAVVGASGL
                     VPRVWHWSYTRHGAPQVHGRFERMHGRRMGEADWGAWVAMKTIGDAVVRTKSLAAADI
                     SAYIRGDQMRVDGSKGPGMSFRPWDNQLRQPVLLSSDVWVVANAPIEGFKHRTNDLDT
                     LGYEERDTTCRF"
     gene            complement(4905..5897)
                     /locus_tag="VGB88_09765"
     CDS             complement(4905..5897)
                     /locus_tag="VGB88_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEX9647774.1"
                     /translation="MTRHEMLAAIGGAALLALGLAGPASAAGTGYIFVSNEADHTVTV
                     VDGKTFQVVKTIATGQRPRDMRWVEGKTRLLVAVSGSDRIDVIDVSKLEVVGAIQAGE
                     DPEIFALDPSGKILVAANEDDNEVTVTDAATGERIRTVEGVGVEPEGVTFRHDGKVVF
                     VTSEATNTVFVIDPWQGEIITEVLVGNRPRRGIVTPDDKEYWVTNELGATVTILDAQT
                     YELIDTVHFEKRGLRQEDISPVDFAMTRDGKTAYITLGRANHVAVVDVASREVTDYIL
                     AGKRVWGAALNADESLLVVTNGASDDITIIDTAKKVAVNSVAVGRTPHTVRIDD"
     gene            complement(5959..6720)
                     /locus_tag="VGB88_09770"
     CDS             complement(5959..6720)
                     /locus_tag="VGB88_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647775.1"
                     /translation="MNEVGATVAQTRVLDARDVVKSYGARKALDHIDIAIAGGEFVAL
                     LGPNGAGKTTLFQLLTGLFVPDHGSIVIDGVDIRRNAVPALAGIGVVFQQPTLDLDLS
                     VEENLLFHANLHGISGAEAERRITQELDRLKLADRRHDRTRALSGGQRRRVELARALV
                     HRPRLLLMDEPTVGLDPASRRDLLSYVLELRQQRRMAILWATHLVDEAERADRVIVLH
                     EGHVLTTGSPAELVSRTGAGDLHDAFVSMTRTDEE"
     gene            complement(6717..7574)
                     /locus_tag="VGB88_09775"
     CDS             complement(6717..7574)
                     /locus_tag="VGB88_09775"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647776.1"
                     /translation="MTGVHAALEVRIDRKVFQAGDGSGERLVIEGLGFEVPAGEFVCI
                     VGPSGCGKTTMLNIIAALDGDATARVRFADGTSLAQARIGYMFQTPRLLPWATVRENV
                     AIVLDGDAGGDGRVEALLERMELGDCLNEYPNRLSGGMQRRVALARAFVAEPSLLLLD
                     EPFVSLDVPVGNRLRSMLLDLWSARPTTVLFVTHDLREAIYLADRILFLSRGPARVIL
                     DTRVPIPRPRGAEDSRIESFRQQLLTQDESLLHGLVSEDAEDTDAPDGQDVDGNGDGA
                     NRPAAGRVA"
     gene            complement(7571..8344)
                     /locus_tag="VGB88_09780"
     CDS             complement(7571..8344)
                     /locus_tag="VGB88_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647777.1"
                     /translation="MIKAASILAAGTSQAWLRAISFVLLLVIWEVAAAAAQSRVLPDP
                     WQVATAFWDHLVNGSLLGDLGITLWRVISAFVLAMVIGTAVGIVMGLRRRVDALLDGW
                     LILFLNIPALVTIILCYVWFGLTEAAAILAVSLNKIPNVVVTIREGSRAIDRDLLEVA
                     RIFRVDRRRTFLRFFLPQLYPYLMIAGRSGIALIWKIVLVVELLGRSNGIGFQLSIFF
                     QQFNIAALLAYTMAFIVVMLLVEIGVVEPLERRMTRWRK"
     gene            complement(8341..9342)
                     /locus_tag="VGB88_09785"
     CDS             complement(8341..9342)
                     /locus_tag="VGB88_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647778.1"
                     /translation="MSLVAVRRAAIAASLAVALALGLADPAAAAGDPPSVRMALLKFG
                     TVNWEVDVIQHHGLDRANGVVLDVVLLANKDATSIALNAADVDMIVTDWLWVSQQRDA
                     GFDFTFVPYSEAAGSVMVHPDAGVDGLMALDGKAMGVAGSPLDKSWLLLRAYSIKQYG
                     KDIAKLVRPAYAAPPLLNEKFAQGELDGVLNFWNYTARLRAQKMTELIRVQDLLPALG
                     VPGRLPLIGYVFSEQWAKDNPEALKSFLAAAAAARKIMVESDAEWERLRPLTGAEDDD
                     TLVALRDGYRDGVPKEFGPVQEQAIRAAFAIVAEVGGRDLVGASTELARGTVWSGNW"
     gene            9562..10341
                     /locus_tag="VGB88_09790"
     CDS             9562..10341
                     /locus_tag="VGB88_09790"
                     /inference="COORDINATES: protein motif:HMM:NF018458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEX9647779.1"
                     /translation="MIDLLGSQFGGVSLPAFPLYQALGKTRDKQFAAFERQPLIEREV
                     AYFLEHAKKVKTVDEFLADRRLMAVALSAFGMEDEIQYLGRMRKVLTEPVADKDSLAN
                     KLIDPRFKKIAEAFQFAELGTFNLGLTSFLNDIAAKYKTNEFERFLGEQNPALREVEY
                     FRRNIGDVESTFNILGDRVLREVVTFALRLPREIVFQSVDKQKALVDARVDIEDFQDP
                     GFVDDFIRQYLILKDSEATQAAFGTGAGGTAAGAYVLDLFA"
     gene            10387..10725
                     /gene="hspQ"
                     /locus_tag="VGB88_09795"
     CDS             10387..10725
                     /gene="hspQ"
                     /locus_tag="VGB88_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX9647780.1"
                     /translation="MSDRAGGPEGARAQFAVGQLVHHRRFDYRGVVFDVDATFQGTDD
                     WYQAMAITRPPKDRPWYHVLVDGAAHTTYVAERNLEPDTEHRPVVHPLVGAVFERFEN
                     GAYLPRRGRN"
     gene            complement(10736..>11053)
                     /locus_tag="VGB88_09800"
     CDS             complement(10736..>11053)
                     /locus_tag="VGB88_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9647781.1"
                     /translation="VPAGPIHDMAEVWSDPHVRARDMLVETEHPRAGTVPNIGVPVKL
                     SETPAAVRKAAPLFGQHTGEVLAEIGVAADELRALHADGVIYDATTAGDLPAHRTAEA
                     AGS"
BASE COUNT         1666 a         3865 c         3856 g         1666 t
ORIGIN      
        1 gacccggtcg atatcgagga acaccgcccc cgggatgtgg cacgcctggt attcggcctc
       61 ggcatcgcgg ccgagatcgg acaggtgata ggtggcgtcg accacccgga ggtcagggtc
      121 gccgagatgg ttggccagcc aatcggtgcc gaccaatgcg ctggtcccgg tttcggtcat
      181 ggcggcacct caggtctcga tcggggtgtc gtcgcgcccg ccccactcgg tccacgagcc
      241 gtcgtagacc gcgacgtccg gcttgccgat caggtgcagc gcaagcgcga ggttgcaggc
      301 ggtgacgccc gagccgcaac tggtcaccac cggtcgcgac aggtcgaggc cggcgtcctt
      361 gaacagcgcg cgcaagtccg cggcctgctt catggtcttg gcctcgccct cgtaaagctc
      421 gagatagggc tggctgagcg cgcccggcat gtgaccgccg cgcaggcccg cgcgcggctc
      481 gggctcggtg ccggcgaagc ggccgcgcga gcgcgcatcg agaatctgct cgctgccgtt
      541 ggcgaccgcg gcgcgcacct ctgccaggcc gcggacgagg gcggcatcga ggcgtgcctt
      601 gtaggtggcc gcgcgcggcg tggccgggcc cctcgccacc ggccggccct cggcgagcca
      661 tttgggcagg ccgccgtcga gcaccgagac gttctcgtgg ccgaaggcgc ggaacgtcca
      721 ccacacccgc gcggcactgc caaccccgac cgtgtcgtag gcgacgacgt gcgcatcgtt
      781 ggcgatgccg agcgcgccca catcgcgggc gaaggtcgcg gcgtccggca gcatgtgcgg
      841 caggggatcg gcgcggtcgg cgatccggtc gatgtcgaaa tacaccgcgc cgggaatgtg
      901 cccggcctcg aactcggcgc gcgcgtcgcg cttgagctgc ggcatgtgcc atgacccgtc
      961 gaccacgcgc agcccgggcg agtccaggtg gtcggcgagc cagcgggtcg acaccagcgc
     1021 ctcgggattg acgtagtcca tgttaaccgt cctccttggc ggcgagcgcg accgtgaccc
     1081 ggcggttgcg gcggcccttg ctttcgatct taatcacccg caccggcccg atctcgccgc
     1141 tgcgccggac atgggtgccg ccgcaggcct gcacgtcgac gcccgcgacc tcgatcaggc
     1201 gcacgctgcc ggcgctgcgc ggcggcttga ccgacatggt cttgaccagg tccgggttgg
     1261 cgtcgagctc agcctcgctc acccagcgcg cgaccagcgg gtggtcctcg gcgatcaggc
     1321 ggttgagcgc agccgagagc gcgtccttgt cgagcccgcc ctcgggcagg tcgaagtcga
     1381 gccggctcct gtcggcgccg acctggccgc cggtgaccgc ccccggcacc agcgccgaca
     1441 gcagatgcag gcagctgtgc atgcgcatca gccggtggcg gcgcggccag tcgatcgacg
     1501 cgctgaccgc ggtgccgggg gcgagcggcg gcgcgccgtc ggcggggacg tggatcacct
     1561 cgtccgccgc ctcgcccttg accgcgtcga cgatctcaac gcgcaccccc gagtcggtct
     1621 ccagcacccc gctatcgccc ggctggccgc cgccgcgggc gtagaacacg gtgcggtcga
     1681 gcctcacccc gcgctcgtcg gccgcgacca cgcgcgcggc acactcggtc aggtacgcgt
     1741 cgtcgcggaa caagagttca gacaccgcgc accggctcct tgtcgagcca gtcgggcacc
     1801 ggcaggtcct tcgagcgcag gaacgccggg ttgaacatct tgctcgcata acgattgccg
     1861 tagtcggcaa ggatggtgac gatggtgtgg ccccgcccca tcgccttggc catcttgacc
     1921 gcgccggcga cgttgatgcc ggacgagccg ccgagcacca gcccctcctc cttggcgagc
     1981 gcgaacacca cctcgagcgc ctccgtatcg gtgacctgct gggcgtcgtc gatcggcgcg
     2041 ccctcgaggt tggcggtgat ccggccctgg ccgatgccct cggtgatcga gtcgcccttg
     2101 atcaccggcg cgcccggctt gagcaggctg tagacctgcg cgcccagcgg gtccgaggcc
     2161 cagacctgaa cgtcgcggtc gcgctccttg agcgccatcg ccaccccggc caaggtgccg
     2221 ccggtgccga ccgcgcaggt aaagccgtcg atgcggccgt cggtctggcg ccaaatctcg
     2281 ggcccggtgc cctcgacatg cgcctggcgg ttggcgacgt tgtcgaactg gttggcccag
     2341 atcgcgccct cgggcccggc ctggttgagc tcggcggcga tccggccgga taccttgacg
     2401 tagttgttgg ggtccttgta ggggaccgcc ggcaccagcc tgagatcggc gccgcacatg
     2461 cgcagcatgt cgatcttctc ctggctctgg gtctcgggga tcacgatcac ggtgcggtag
     2521 ccgagcgcgt tgccgaccag cgccaggccg atgccggtgt tgcccgcggt gccctcgacg
     2581 atcacgccgc ccttgcgaag cgtgccgcgg gcctcggcgt cgcggacaat gaacagcgcc
     2641 gcccggtcct tgaccgactg gcccggattg aggaactcgc acttgcccag gatctcgcaa
     2701 ccggtctcgc gcgaggcgcg cgccaggcgg atcagcggcg tgttgccgat caggtcgacg
     2761 aagccctcct tgacctccat ggccggccct ccttggtcgt cagcggcggg gtcaggccgc
     2821 ctgcgggccg cggcggacga agccgcgctg cgggtcgtag ccgatgatcg cggcgatcag
     2881 gaacaccagc gtggtcgcgc acaccaccac cgcgccgagg ccgtagagct ggccctcgct
     2941 ggcatagcgg accagctgca cgccataggt gaacgggttg gcgttggcga cccagtagac
     3001 gatgtcggcg ccgctctcct gcaggtacca gagcgggaac agcgccggcg agatgaagaa
     3061 catcgggaag atgacaaagt tcatcgtgcc ggcgaagttc tcgagctgct tgatgtgcac
     3121 cgacaagagc aggccgagcg cgccgagcat catcccggtc atgatcagcg ccggcaggac
     3181 gtacagccac gccagcgggt cgttgaacgc gacgtgcagg ctggtcaacc agtcgaacgg
     3241 caggcagatg accaggaacg cgtaggcctg gagcaccgac aggaaggtgc ccgagacgag
     3301 cttggcggtg agcaggtacc agcgcggcaa cggcgcggtc agcagcagcc gcatcactcc
     3361 catctcgcgg tcgtagacca tcgacagcga cgactgcatg ccgctgaaca gcagcaccat
     3421 gcaggtcagc ccgggcagga tgtagtactc gtactcgatg ttgcgcgcga gatagggctc
     3481 gccggcgtcg accccgtaga cgccgcggaa cccgaccgcg aacacgaaca gccacagcgc
     3541 cgggcggacg aaggcggaga acagccggcc cttctgctgc acgaacttga tcagctcgcg
     3601 ccggctgagc gcgccgacgg cggccaaggc atggctggct tgcatggctg gcgtgatcct
     3661 ccccgctggc atgccgcccg gtcagaagcg gcaggtggtg tcccgttcct cgtaaccgag
     3721 ggtgtcgagg tcgttggtgc ggtgcttgaa gccctcgatc ggcgcgttgg ccaccaccca
     3781 tacgtcgctc gacagcagca ccggctggcg cagctggttg tcccacggcc ggaagctcat
     3841 tcccggaccc ttggagccgt cgacccgcat ctggtcgccg cgaatgtagg ccgagatgtc
     3901 ggccgcggcc agcgacttgg tccgcaccac cgcatcgccg atggtcttca tcgccaccca
     3961 cgcgccccag tcggcctcgc ccatgcgccg gccgtgcatg cgctcgaagc ggccgtgcac
     4021 ctgcggcgcg ccgtggcggg tgtacgacca gtgccagacc cgcggcacca gcccggatgc
     4081 gcccaccacc gcggccggct gcagagtgtc gtagggaact gcgagcgcga actcgccgtc
     4141 ggcgtcggcg accatcaccg cgtcgtagct ggcgttgccg gtgagaaagg caaggtcgtt
     4201 gagctcgcgc gcgcgcgggt cgctgccgag cacgaagctg cgggtctcgg tgaccgcgac
     4261 gccgaacagc ttggcggcgc gcttgaatgc ggacgcggta agcgcgtcct cggccaacgg
     4321 tccctggagg atgagcacgc gcgtccactt cttggccttg aggaactggg cgaggccgtc
     4381 catcagcatc gcccggctgg cggcggtgtg gaacaggtgc ggcgcgcagt cgtcgttgcg
     4441 cagcgaatcg ccggtcgcgg tggcgttgaa cagcagcacg ccgctgtcgc gggtcgcctt
     4501 ggcgacctcc gccacggcgg cgtcgggcag gtcgaggacg aacaggctga cgccgtccgc
     4561 ggccaggccc ttgatcgcct cgatcgccgc cgccgcgtcg gccaccgtcc gccgctcgag
     4621 cgcgaagttc gccttggcga cccgcttgag ctgcgccacg tcggcgaggc cgagctcagc
     4681 gccggcccag gcgtgccggt cgacgatcgc ggtcgccgag cggatgtcga ccgggtgcag
     4741 cccccagtcg tcgtagcgca cgtccggttc gagatcgaca tagccgatgg tcagcgtcgg
     4801 cagctcgttc tggtcgggct cggcagccgg caccggcacg ggcgccgccg cgacgatggc
     4861 gagcagcaga agtgcagcga cgaacgggcg aaacatctgt tgcgctagtc gtcgatgcgc
     4921 acggtatgcg gcgtgcgccc gaccgcgacc gagttcacgg ccactttctt ggcggtgtcg
     4981 atgatggtga tgtcgtcgct ggcgccgttg gtgaccacga gcagtgactc atcggcgtta
     5041 agcgccgcgc cccacacgcg ctttccggcc aggatgtagt cggtcacctc gcggctcgcc
     5101 acatcgacga cggcgacgtg gttggcgcgc ccgagcgtga tgtaggcggt cttgccgtcg
     5161 cgggtcatgg cgaagtccac cggcgagatg tcctcctggc gcaggccgcg cttctcgaaa
     5221 tgcaccgtgt cgatcagctc gtaagtctgc gcgtcgagga tggtgaccgt ggcgccgagc
     5281 tcgttggtca cccagtactc cttgtcgtcg ggcgtgacga tgccgcggcg cggccggttg
     5341 ccgaccagca cctcggtgat gatctcgccc tgccagggat cgatcacgaa cacggtgttg
     5401 gtcgcttccg aggtgacgaa caccaccttg ccgtcgtggc ggaaggtcac gccctcgggc
     5461 tcgacgccga cgccctcgac ggtgcggatg cgctcgccgg tggcggcgtc ggtcaccgtc
     5521 acctcgttgt cgtcctcgtt cgccgcgacc aggatcttgc cgctcgggtc gagggcgaag
     5581 atctccgggt cctcgccggc ctggatggcg ccgaccacct cgagcttgga cacatcgatg
     5641 acgtcgatgc ggtcgctgcc gctgaccgcg accagcagcc tggtcttgcc ctcgacccag
     5701 cgcatgtcgc gcgggcgctg gccggtggcg atggtcttga ccacctggaa ggtcttgccg
     5761 tcgaccacgg tgacggtgtg atcggcctcg ttgctgacga agatgtagcc ggttccggcc
     5821 gccgatgccg gcccggcgag gccgagggcg agcaaggcgg cacctccgat tgccgcgagc
     5881 atctcgtgtc tggtcatcgt ctccgtctcc gtccgttcgg cgaccgcgcc gagccggcgc
     5941 gctgggcgtg gcgcctttct actcctcgtc cgtccgggtc atcgagacga aggcatcgtg
     6001 aaggtcgccg gcgccggtcc ggctgaccag ctccgccggg ctgccggtgg tcagcacgtg
     6061 cccctcgtgg agcacgatca cgcggtcggc gcgctccgcc tcgtcgacca ggtgtgtggc
     6121 ccacaggatc gccatccggc gctgctgcct gagctcgagg acgtaggaca acaggtccct
     6181 gcggctggcc gggtcgaggc cgacggtcgg ctcgtccatc agcaacagcc gcggccggtg
     6241 gaccagggcg cgcgccagct ccacgcgccg cctctggccg ccgctcagcg cgcgcgtgcg
     6301 gtcgtgccgg cgatcggcga gcttgaggcg gtcgagctcc tgggtgatgc gccgctccgc
     6361 ctcggcgccg ctgatgccgt ggaggttggc gtggaacagg aggttctcct cgaccgacag
     6421 gtcgaggtcg agggtcggct gctggaacac gacgccgatc ccggccagtg ccggcaccgc
     6481 gttgcgccgg atgtcgacgc cgtcgatgac gatgctgccg tggtcgggca cgaacaggcc
     6541 ggtcagcagc tggaacaggg tggtcttgcc ggcaccgttg gggccgagca gggcgacgaa
     6601 ctccccgccc gcgatggcga tgtcgatgtg gtcgagcgcc ttgcgcgcgc cgtagctctt
     6661 gaccacgtcg cgggcatcga gaacccgggt ctgggccacc gtggcgccga cctcgttcac
     6721 gccaccctcc ccgcggccgg ccggtttgcg ccatcgccgt tgccgtccac atcctggccg
     6781 tcgggcgcgt cggtgtcttc cgcgtcctcg gacaccaggc cgtgcagaag cgactcgtcc
     6841 tgcgtcagca gctgctggcg gaagctttcg atgcgcgagt cctcggcgcc acgcggccgc
     6901 gggatcggta ctcgcgtgtc caggatcacg cgcgccgggc cgcgcgacag gaacaggatg
     6961 cggtcggcga ggtagatcgc ctcgcgcaga tcgtgggtca cgaacagcac cgtggtcggg
     7021 cgcgcgctcc acaggtcgag cagcatgctc cgaagccgat tgccgaccgg cacgtcgagc
     7081 gagacgaacg gctcgtcgag cagcaaaagg ctcggctcgg cgacgaaggc gcgcgccagc
     7141 gccacgcgcc gctgcatgcc gcccgacagg cggttgggat actcgttcag gcaatcgccg
     7201 agctccatcc gctcgagcag ggcctcgacc cgtccgtcgc cgcccgcgtc cccgtcgagc
     7261 acgatggcga cgttctcgcg caccgtcgcc cacggcagca gccgcggggt ctggaacatg
     7321 tagccgatcc gcgcttgggc gaggctggtg ccgtcggcga agcggacccg cgccgtggcg
     7381 tcgccgtcga gcgcggcgat gatgttgagc atggtggtct tgccgcagcc cgacggcccg
     7441 acgatgcaca cgaactcgcc ggccgggacc tcgaagccga ggccctcgat caccagccgc
     7501 tcgccgctgc cgtcgccggc ctggaagacc ttgcggtcga tccggacctc gagtgccgcg
     7561 tgcaccccgg tcatttgcgc caccgggtca tgcgccgctc gagcggctcg accacgccga
     7621 tctcgaccag cagcatcacg acgatgaagg ccatcgtgta ggcgagcagg gcggcgatgt
     7681 tgaactgctg gaagaagatg ctgagctgaa agccgatgcc gttgctgcgc cccaacagct
     7741 cgaccaccag cacgatcttc cagatcagcg cgatgcccga gcggccggcg atcatcagat
     7801 aggggtagag ctgaggcagg aagaagcgca ggaacgtgcg ccgccggtcg acccggaaga
     7861 tgcgcgccac ctccagcagg tcgcggtcga tggcgcgcga cccttcgcgg atggtgacga
     7921 cgacgttggg gatcttgttc agcgacaccg ccaggatcgc ggcggcctcg gtcaaaccga
     7981 accagacgta gcacaggatg atggtgacca gcgccgggat gttgaggaac aggatcagcc
     8041 agccgtcgag cagcgcatcg acccggcgcc tgagccccat gacgatgccg accgcggtac
     8101 cgatcaccat cgccagcacg aacgccgaga tcacccgcca gagcgtgatg ccgaggtcgc
     8161 cgagcaggct gccgttgacc agatgatccc agaatgcggt cgccacctgc cacggatcgg
     8221 gcagcacccg cgactgggcg gcggccgcgg ccacctccca gatcaccagc agcaggacga
     8281 aggagatcgc cctcagccat gcttgactcg tcccggccgc cagtatggac gcggctttga
     8341 tcaccagttc ccgctccaga ccgtgccccg cgccagctcg gtgctggcgc cgaccaggtc
     8401 gcggccgccg acctcggcga cgatcgcgaa cgcggcacgg atcgcctgct cctggaccgg
     8461 cccgaactcc ttcggcacgc cgtcgcgata gccgtcgcgg agcgcgacca gggtatcgtc
     8521 gtcctcggcg ccggtcagcg gcctgagccg ctcccattcg gcgtccgact cgaccatgat
     8581 cttgcgcgcc gccgcggcgg cggcgaggaa gctcttgagc gcctcggggt tgtccttcgc
     8641 ccactgctcg ctgaacacgt agccgatcag cggcaggcgg ccgggaacgc cgagggcggg
     8701 cagcaggtcc tggacccgga tcagctcggt catcttctgc gcgcgcagcc gcgcggtgta
     8761 gttccagaag ttgagcacgc cgtcgagctc gccctgcgcg aacttctcgt tcaacagcgg
     8821 cggggcggca taggcgggcc ggaccagctt ggcgatgtcc ttgccgtatt gcttgatcga
     8881 gtacgccctg agcagcagcc agctcttgtc gagcgggctg ccggcaacgc ccatggcctt
     8941 gccgtcaagc gccatcaggc cgtcgacgcc ggcatcgggg tggaccatca ccgagccggc
     9001 ggcctcggaa taggggacga aggtgaagtc gaagccggcg tcgcgctgct gcgagaccca
     9061 cagccagtcg gtcacgatca tgtcgacgtc ggcggcattc agcgcgatcg aggtggcgtc
     9121 cttgttggcg agcagcacca cgtcgagcac gacgccgttg gcgcggtcga ggccatgatg
     9181 ctggatgacg tcgacctccc agttgacggt gccgaacttg agcagcgcca tgcgcaccga
     9241 cggcgggtcg cccgcggccg cggccggatc ggcgaggccg agcgccaggg ccacggccag
     9301 ggacgcggca atggcggcgc ggcggacggc aacaagactc atcgcgtttc ccctgttccg
     9361 agcatttccc cgcggccact gaaccagcgg tggccggacc cccccgggtc cggccaccca
     9421 cgaagtcgcc ccttatatag tacgtgccgg tggcggaaaa gaggcccggg cgggccggcc
     9481 gccccggcat ttgcctcgct gcgccagcgg tccatatcgt tgatcacggg gacgggacca
     9541 ggcggagccc ggcgagggat catgatcgac ctgcttggaa gccagttcgg cggcgtatcg
     9601 ctccccgcgt ttccgctcta ccaggcgctc ggcaagaccc gcgacaagca gttcgcggcg
     9661 ttcgagcgcc agccgctgat cgagcgcgag gtcgcctact tcctggagca cgccaagaag
     9721 gtcaagacgg tcgacgagtt cctcgccgac cggcggctga tggcggtggc gctgtcggcg
     9781 ttcggcatgg aggacgagat ccagtatctc gggcgcatgc gcaaggtgct gaccgagccg
     9841 gtcgccgaca aggactcgct cgccaacaag ctgatcgacc cgcgcttcaa gaagatcgcc
     9901 gaggcgttcc agttcgccga gctcggcacc ttcaacctcg gcctgaccag cttcctcaac
     9961 gacatcgccg ccaagtacaa gaccaacgag ttcgagcgct tcctcggcga gcaaaacccg
    10021 gccctgcgcg aggtcgagta tttccgccgc aacatcggcg acgtcgagag caccttcaac
    10081 attctcggcg accgggtgct gcgcgaggtg gtgaccttcg ctctgcgatt gccgcgcgag
    10141 atcgtgttcc agtcggtcga caagcagaag gcgctggtcg atgcgcgggt cgacatcgag
    10201 gacttccagg accctgggtt cgtcgacgat ttcatccgcc agtacctgat cctcaaggac
    10261 tccgaggcga cccaggccgc gtttggcacc ggcgccggcg gcacggctgc gggcgcctac
    10321 gtcctcgacc tgttcgcctg agcccccggc tcgcggccgc atgcggcggc tgctacactg
    10381 atggacatga gcgaccgagc aggcgggccc gagggcgcgc gcgcccagtt cgcggtcggg
    10441 cagctggtcc accaccgccg gttcgactat cgcggcgtgg tgttcgatgt cgacgcgact
    10501 ttccagggca ccgacgactg gtaccaggcg atggcgatca cccgcccgcc caaggaccgg
    10561 ccctggtatc acgtgctggt cgacggcgcc gcccacacca cctacgtcgc cgagcgcaac
    10621 ctggagccgg acaccgagca ccggcctgtt gtccacccgc tggtcggcgc ggtgttcgag
    10681 cggttcgaga acggcgccta tctgccccgg cgcgggcgca actgagtcga taggtttacg
    10741 acccggcggc ctccgccgtg cggtgcgccg gcaggtcgcc cgcggtcgtc gcgtcgtaga
    10801 tcacgccgtc cgcgtgcagc gcccgcagct cgtccgccgc gacgccgatc tcggccagca
    10861 cctcgccggt gtgctggccg aacagcggcg ccgccttgcg caccgccgcc ggggtctcgg
    10921 acagcttcac cggcacgccg atattgggca cggtgccggc gcgcgggtgc tcggtctcga
    10981 ccagcatgtc gcgggcgcgc acgtgagggt cgctccatac ctcggccatg tcgtggatcg
    11041 gcccggccgg gac
//
LOCUS       DASWHU010000265         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3455728, whole genome shotgun sequence.
ACCESSION   DASWHU010000265 DASWHU010000000
VERSION     DASWHU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455728"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1174)
                     /locus_tag="VGB88_09805"
     CDS             complement(146..1174)
                     /locus_tag="VGB88_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9647782.1"
                     /translation="MTQPVNRRIVLARRPAGEPTPADFRLEQAPVPAPAPGEVLVRTV
                     YLSLDPYMRGRMNAGPSYAPPVEVGGVMEGGAVSVVEASSHPGYAAGDMVVGRTGWQD
                     YAVADSKAVRKLDASAAPVSTALGVLGMPGLTAYTGLLNIGKPQAGETVAVAAATGAV
                     GSVVGQIAKIKGCRAVAIAGGADKCAFAREELGFDAAVDHRAPDLAQRLKGACPDGID
                     VYFENVGGAVLNAVVPLMNPFSRMPVCGLIAHYNDAAPPPGPDRLPGFLMRVLANRIT
                     VRGFIVRDFADQHADFQRDVGGWLREGRIRYREDFIDGLENAPAGLIGLLKGNNFGKL
                     VVRVSPEP"
     gene            1288..>1730
                     /locus_tag="VGB88_09810"
     CDS             1288..>1730
                     /locus_tag="VGB88_09810"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9647783.1"
                     /translation="MAIAIRPLHPSFAAEVTGVDLSEPLDDATVAAIRAAFNDRAVLA
                     FPGQPLTDDQQIAFSLRFGPLEKTITSAMGGTAVISNLSNTDAEGRLYQTDDARALFL
                     SGNQRWHSDSSFKVVPAMASLLSAREVPPEGGETEYADMRAAYDAL"
BASE COUNT          230 a          639 c          622 g          239 t
ORIGIN      
        1 gccttcgagg tcggcgtctt cgggctgatc ggcggcgcgc cgatgcgcga actgctggcg
       61 gcgctcgatc ccgcgaccgg caacctgttc gcgctcgcgc tcctcgccac cctggccggc
      121 ccgcggctga tggcggcggc ccgcctcaag gctcgggcga gaccctgacc acgagcttgc
      181 cgaagttgtt gcctttcagc agcccgatca ggcccgcggg cgcgttctcc aggccgtcga
      241 tgaagtcctc gcgatagcgg atgcggccct cgcgcagcca gccgccgacg tcgcgctgga
      301 agtccgcgtg ctggtcggcg aagtcgcgga cgatgaagcc gcgcacggtg atgcggttgg
      361 cgagcacgcg catcaggaag ccgggcagcc ggtcggggcc gggcggcggc gcggcgtcgt
      421 tgtagtgcgc gatcagcccg cacaccggca tgcgcgagaa cgggttcatc agcggcacca
      481 cggcgttgag caccgcgccg ccgacattct cgaagtagac gtcgatgccg tccgggcagg
      541 cccccttgag gcgctgggcg aggtcgggcg cgcggtggtc gacggcggcg tcgaagccga
      601 gctcctcgcg cgcgaaggcg cacttgtccg cgccgccggc gatcgccacc gcgcggcagc
      661 ccttgatctt ggcgatctgg ccgacgacgc tgccgaccgc gccggtggcg gcggccaccg
      721 ccacggtctc gccggcctgg ggcttgccga tgttcaacag cccggtatag gccgtcagcc
      781 ccggcatgcc gagcacgccg agcgcggtcg acaccggtgc cgcgctcgca tccagcttgc
      841 gcacggcctt gctgtcggcg accgcgtagt cctgccagcc ggtgcgcccg accaccatgt
      901 cgccggcggc atagcccgga tggctcgacg cctcgaccac gctcaccgcg ccgccttcca
      961 tcacgccgcc gacctcgacc ggcggcgcgt aggatgggcc cgcgttcatc cgcccgcgca
     1021 tgtaggggtc gagcgacaga tagaccgtgc gcaccagcac ctcgccgggc gcgggcgcgg
     1081 gcaccggcgc ctgctcgagc cggaagtcgg cgggcgtggg ctcgccggcc gggcggcggg
     1141 caagcacgat gcggcggttg acgggctggg tcacggcggc ctccctcggg ctcgagaact
     1201 catgctagga taggccgagc cgccgcccgt gtggtgggct tttcggcccc gccggcggtt
     1261 gcacgtgaaa cagcgagggt gcccgccatg gcgatcgcga tccgccccct tcatccctcc
     1321 ttcgccgccg aggtgaccgg cgtcgacctg agcgagccgc tcgacgacgc gaccgtcgcc
     1381 gccatccgcg ccgcgttcaa cgaccgcgcg gtgctggcct tcccgggcca gccgctcacc
     1441 gacgaccagc agatcgcgtt cagcctgcgc ttcgggccgc tggagaagac catcaccagc
     1501 gccatgggcg gcaccgcggt gatctcgaac ctctccaaca ccgacgccga gggccggctg
     1561 taccagacgg acgatgcgcg tgcgctgttc ctgtccggca accagcgctg gcacagcgac
     1621 agctcgttca aggtggtgcc ggcgatggcc tcgctgttgt ccgcgcgcga ggtgccgccg
     1681 gagggcggcg agaccgagta cgccgacatg cgcgcggcct acgatgcgct
//
LOCUS       DASWHU010000266         6144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2383696, whole genome shotgun sequence.
ACCESSION   DASWHU010000266 DASWHU010000000
VERSION     DASWHU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383696"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGB88_09815"
     CDS             complement(<1..597)
                     /locus_tag="VGB88_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647784.1"
                     /translation="MAERQHLVLCPGLLCDHALWSAQSRALSDLAAPSVADFTRAESI
                     ADMARSVLDAAPDRFALAGLSMGGYVAQEVIRRAPGRVTRLALLDTLARPESGEQTRR
                     RRELLALAARGRFDEVTPRLLPLLVAADRLADEALCGAIAAMAGRVGPEAFARQQQAI
                     MGRADGRGELPRIACPTLVLCGREDRLTPVEHHTELAER"
     gene            complement(611..835)
                     /locus_tag="VGB88_09820"
     CDS             complement(611..835)
                     /locus_tag="VGB88_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9647785.1"
                     /translation="MKINSSTAFLLVIDVQERLVPAMHQAERMVANCAKLLRAAPALG
                     LPVVATEQVPCECMGRAGTNLFKQVSALIR"
     gene            958..2406
                     /locus_tag="VGB88_09825"
     CDS             958..2406
                     /locus_tag="VGB88_09825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9647786.1"
                     /translation="MSDVTLRAVKDAGYLTPTPIQAKAIPIILQGRDVLGCAQTGTGK
                     TASFTLPMVDILSSGRARARMPRSLILIPTRELATQIAENFETYGKYHKLSTALLIGG
                     VSMTDQQRALERGVDVLIATPGRLLDLFERGNILLSGVKILVIDEADRMLDMGFIPDV
                     ERIVGLLPRMRQTMMFGATMPAEIRRLADRFLMSPKEITVAPPASPAETVSHAVSIVR
                     GTDKEAALCALIRHQPVENALIFCNRKKDVDVLDRSLRRHGLSSAALHGDMSQPARTE
                     TLEAFKAGKITLLVATDVAGRGLDIEGLSHVFNYDVPLHAEDYVHRIGRTGRAGRTGR
                     AFMIATPADGKYLDAIERLIKRPVARIEIEGIEPAEVGEAEAGRGGRRRRKPTPEAAK
                     RAAKPAPAPAKGKATAEAPAEAEAGAKRRPSRRRRAEAKDSRAPAKPRQAEDRPRQDR
                     PRQEKTGGDGKVVGMGDHVPAFMLRKPPRTDG"
     gene            2447..2689
                     /locus_tag="VGB88_09830"
     CDS             2447..2689
                     /locus_tag="VGB88_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9647787.1"
                     /translation="MQTIFVSVKCELGKAYEVANELVQTVEEVSEVHSTSGKYDLLVK
                     AYLADDRDIGHFVTERVQRLPGVADTFTTIAFKAFT"
     gene            2720..4075
                     /locus_tag="VGB88_09835"
     CDS             2720..4075
                     /locus_tag="VGB88_09835"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9647788.1"
                     /translation="MAQRFDLIVHGGTVVTPVGHDAADIGVVAGHIAEIGNLAQADAG
                     HRLDAHGLHVLPGVIDSQVHFREPGGEHKEDLATGTAAAALGGVTAVFEMPNTDPPTT
                     SAAALADKLARARGRAWVDHAFFIGAAPDNLDRLAELERLPGCCGVKIFMGSSTGTLL
                     LADDANVERALRAGRRRVAVHAEDEARLKERRRLAESGGGPELHPEWRDVECARRATE
                     RLLALAKRTRRPVHVLHVSTAEEMALLATRPKWVSVECTPQHLTLEAPDCYCRLGTLA
                     QMNPPIRDARHRAALWAGLAGGVVDVIGSDHAPHSLAEKARPYPESPSGMPGVQTLLP
                     IMLDHVHAGRLGLDRLVELVSGAPARLFQVERKGAIRVGFDADLSLVDLAARREITDD
                     WLASKCGWSPFAGTTVTGWPMATVVRGQVVMRDGELVGRPAGAPVRFADLIADAAAGG
                     A"
     gene            4183..4758
                     /locus_tag="VGB88_09840"
     CDS             4183..4758
                     /locus_tag="VGB88_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647789.1"
                     /translation="MAGFAALPASAQDFDVPEGFASEIVRDLGTAGEPRAILRIHPED
                     GAFSGLSTVELEPVAGDIDDPVAWLRERMIARFEDLVPDPEAMMDNPDSPFGDPSFAE
                     LREAMSDLITRLAELGRLPLEYCDDPSELHNRAGSLHELSCRFTVGPISHYQVLRLQN
                     VGGLWYYTRIRTMNERRLRHLLAIANSFHTD"
     gene            4769..5995
                     /locus_tag="VGB88_09845"
     CDS             4769..5995
                     /locus_tag="VGB88_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9647790.1"
                     /translation="MTSQPERELPAPAAPRAAGAAFDWADPFLLADQLSEEERMIAET
                     ARAFAQRRLMPGIVEANRHERFERALMAEMGALGLLGVTLEGYGCAGASSVAYGLVAR
                     EIERVDSAYRSCLSVQSSLVMWPIHAYGTEAQRRKYLPGLARGDLIGCFGLTEPDHGS
                     DPGGMISRARAAAGGFRLSGTKTWITNSPIADLAVVWAKDDDGHIRGFVLERGMDGLA
                     TPKIEGKFSLRASPTGQIVMDDVLVPAENAFPDIRGLAGPFGCLNNARYGIAWGAMGA
                     AEFCWQAARDYVLDRKQFGRPLAANQLIQKKLADMQTEITLGLTACLRLGRLMDADRA
                     TPEMISMLKRNNAGKALDVARMARDMHGGNGISDEFHVIRHLVNLETVNTYEGTHDIH
                     ALILGRAQTGIQAFTG"
BASE COUNT          949 a         2249 c         2145 g          801 t
ORIGIN      
        1 ccgctcggca agctccgtgt ggtgctcgac cggggtcagg cgatcctcgc gcccgcacag
       61 caccagcgtc gggcacgcga tccggggcaa ctcgccgcgg ccgtcggcgc ggcccatgat
      121 cgcctgctgc tggcgcgcga acgcctcagg cccgacgcgc cccgccatgg cggcgatggc
      181 gccgcacagt gcctcgtccg cgagccggtc cgcggccacc agcagcggca gcaaccgcgg
      241 cgtgacctcg tcgaaccggc cgcgcgcggc aagcgccagc agctcgcggc gacgccgcgt
      301 ctgctccccc gattccggcc gcgccaaggt gtcgagaaga gccaggcgcg tgacgcgacc
      361 gggcgcgcgc cggatgacct cctgcgccac gtagccgccc atcgacagcc cggcgagggc
      421 gaacctgtcc ggcgcggcgt cgagcacgga gcgggccatg tcggcgatcg actcggcgcg
      481 ggtgaagtcc gccaccgacg gcgcggccag gtcggacagc gcccgcgact gtgctgacca
      541 cagggcgtgg tcgcacagca gcccgggaca caggaccaga tgctgccgct cggccatttc
      601 cgcgccccgg tcagcggatc agggcgctca cctgcttgaa caggttggtc ccggcccggc
      661 ccatgcactc gcacggcacc tgctcggtcg ccaccaccgg caagcccagg gccggcgcgg
      721 cgcgcaacag cttggcgcaa ttggcgacca tgcgctcggc ctgatgcatc gccggcacca
      781 gccgctcctg gacgtcgatg accaggagga atgccgttga actgttgatt ttcatcgaat
      841 cagtcccgat attgccggca gatgcaggaa actccttcta ctctttctga gaccgagacg
      901 tccattccca gcccgagcga gaccgactcc ggcccggcaa gtttcgatga cctgggcttg
      961 agcgacgtta cgctgagggc ggtcaaggac gccggctacc tgaccccgac cccgatccag
     1021 gccaaggcga tcccgatcat cctgcagggg cgcgacgtgc tgggctgcgc ccagaccggc
     1081 accggcaaga ccgcgtcgtt caccctgccg atggtcgata tcctgagctc ggggcgggcg
     1141 cgggcgcgca tgccgcgctc gctgatcctc atcccgaccc gcgagctcgc cacccagatc
     1201 gcggaaaatt tcgagaccta cggcaagtac cacaagctgt cgacggcgct gctgatcggc
     1261 ggcgtgtcga tgaccgacca gcagcgcgcg ctggagcgcg gcgtcgacgt gctgatcgcc
     1321 accccggggc gcctgctcga cctgttcgag cgcggcaaca tcctgctctc gggagtcaag
     1381 atcctggtca tcgacgaggc cgaccgaatg ctcgacatgg gcttcatccc ggatgtcgag
     1441 cggatcgtcg ggctgttgcc gcggatgcgc cagacgatga tgttcggcgc caccatgccg
     1501 gccgagatcc ggcgcctggc ggacaggttc ctgatgtcgc cgaaggagat cacggtggcg
     1561 ccgccggcgt cgcccgcgga aaccgtctcg cacgcggtct cgatcgtgcg cggcacggac
     1621 aaggaagcgg cgctgtgcgc cctgatccgc caccagccgg tcgagaacgc gctgatcttc
     1681 tgcaaccgca agaaggacgt agacgtgctc gaccgttcgc tgcgccgcca cggcttgtcg
     1741 agcgcggcgc tgcacggcga catgtcccag ccggcgcgga ccgagacgct cgaggcgttc
     1801 aaggcgggca agatcacgct gctggtcgcc accgacgtcg ccgggcgcgg cctcgacatc
     1861 gagggcttga gccacgtgtt caactacgac gtgccgctgc acgccgagga ttacgtccac
     1921 cgcatcggcc gcaccgggcg cgccgggcgc accgggcgcg cgttcatgat cgcgaccccc
     1981 gccgacggca agtatctgga cgccatcgag cgcctgatca agcggccggt tgcgcgcatc
     2041 gagatcgagg gcatcgagcc cgccgaggtc ggcgaggccg aggccggccg cggcggccgg
     2101 cggcgccgaa agccaacgcc ggaagccgcc aagcgggcgg cgaagccggc gccggcgccg
     2161 gcgaagggca aggcgacggc cgaggccccc gccgaggccg aggccggtgc caagcgccgc
     2221 cccagccgcc ggcggcgggc cgaggcaaag gactcgcgcg ctcccgccaa gccccgccag
     2281 gccgaggacc ggccgcgcca agaccggccg cgccaagaga agaccggcgg cgacggcaag
     2341 gtcgtcggca tgggcgacca cgtgcccgcg ttcatgctaa gaaagccacc acgcaccgac
     2401 ggctgagtcg ccggcgcgtg cacggtcggg tgcgactggg gcggccatgc agaccatctt
     2461 cgtgtcggtt aagtgcgagc tcggcaaggc ctacgaggtg gccaacgagc tggtgcagac
     2521 ggtcgaggag gtctccgagg tccactcgac ctcgggcaag tacgacctgc tggtcaaggc
     2581 ctacctggcg gacgaccgcg acatcggcca cttcgtgacc gagcgggtgc aacgcctgcc
     2641 cggggtcgcc gacaccttca ccaccatcgc gttcaaggcc ttcacctgag gccgccggcg
     2701 cgagcgccgg aaatatggca tggcccagcg gttcgacctg atcgtccacg gcggcaccgt
     2761 ggtcaccccg gtcggccacg acgctgccga catcggcgtg gtcgccggcc acatcgccga
     2821 gatcggcaac ctcgcccagg ccgatgccgg gcaccgactc gacgcgcacg gactgcacgt
     2881 cctgcccggg gtgatcgaca gccaggtgca tttccgcgag cccggcggcg agcacaagga
     2941 ggacctggcg accggcaccg ctgccgccgc gctgggcggc gtgaccgcgg tgttcgagat
     3001 gcccaacacc gatccaccga cgaccagcgc ggcggcgctc gccgacaagc tcgcccgcgc
     3061 ccgcggccgc gcctgggtgg accacgcctt cttcatcggc gccgcgcccg acaacctcga
     3121 ccggctggcc gagctcgagc gcctgcccgg ctgctgcggg gtcaagatct tcatgggctc
     3181 gtcgaccggc acgctgctgc tcgccgacga cgccaatgtc gagcgcgcgc tgcgcgccgg
     3241 ccggcgccgg gtcgcggtgc acgccgagga cgaggcgcgg ctcaaggagc ggcgcaggct
     3301 cgcggagtct ggcggtgggc cggagctgca ccccgaatgg cgcgatgtcg agtgcgcgcg
     3361 gcgcgccacc gagcggctgc tggcgctggc caagcgcacg cgccggccgg tgcacgtgct
     3421 gcacgtgtcc accgcggagg agatggcgct gctcgcgaca cggccgaaat gggtcagcgt
     3481 cgagtgcacg ccgcagcacc tcaccctcga ggcgccggac tgctattgcc ggctcggcac
     3541 cctcgcccag atgaacccgc caatccgcga cgcgcgccac cgggcggcgc tgtgggcggg
     3601 actcgccggc ggcgtggtcg acgtgatcgg ctccgaccac gctccgcaca gccttgcaga
     3661 gaaggcgcgg ccctaccccg agagcccgtc gggaatgccc ggcgtgcaga ccttgctgcc
     3721 gatcatgctc gaccacgtcc atgccggccg gctcggcctc gaccggctgg tggagctggt
     3781 ctcgggcgct cccgcccgcc tgttccaggt cgaacgcaag ggcgcgatcc gggtcggctt
     3841 cgacgccgac ctcagcctgg tcgacctcgc cgcccggcgc gagatcaccg acgactggct
     3901 ggcgagcaaa tgcggctgga gcccgttcgc cggcaccacc gtcaccggct ggccgatggc
     3961 gaccgtggtg cgcggccagg tggtgatgcg cgacggcgag ctggtcggcc ggccggcggg
     4021 cgcgccggtc cgcttcgccg acctgatcgc cgacgcggcg gcgggcggcg catgagcgcc
     4081 attgacccgc gcgcggccgg ccaccacgtg gcatcggtca tggacgccga tggcatacgc
     4141 tcgcgcgcgg cttgcggact ccgcgcggcg ctggttgcgg tcttggcggg attcgcagcc
     4201 ctgcccgcct cggcgcagga cttcgacgtt cccgaaggat tcgccagcga gatcgtgcgc
     4261 gacctcggca ccgccggcga gccgcgcgcg atcctgcgca tccatcccga ggacggcgcg
     4321 ttctcgggcc tgagcacggt cgagctggag ccggtggcag gcgacatcga cgatcccgtc
     4381 gcctggctgc gcgagcgcat gatcgcccgc ttcgaggacc tggtgccgga ccccgaggcg
     4441 atgatggaca accccgacag cccgttcggc gacccgagct tcgccgagct gcgcgaggcg
     4501 atgtcggact tgatcaccag gctcgccgag ctcggccggc tgccgctgga gtactgcgac
     4561 gaccccagcg agctgcacaa ccgggccggc agcctgcacg agctgtcgtg ccggttcacc
     4621 gtcgggccga tcagccacta ccaggttctc aggctgcaga acgtcggcgg gctgtggtac
     4681 tacacgcgca tccgcaccat gaacgagcgc cgcctgcgtc atctgctcgc gatcgcgaac
     4741 tcgttccaca ccgactgagc ttggcgccat gaccagccag cccgagaggg agctccctgc
     4801 gcccgccgcg ccgcgcgcgg ccggcgccgc cttcgactgg gccgatccgt tcctgctcgc
     4861 cgaccagctg agcgaggagg aacgcatgat cgccgagacc gcgcgcgcgt tcgcgcaacg
     4921 gcggctgatg ccgggcatcg tcgaggccaa ccgccacgag cgcttcgagc gcgcgctgat
     4981 ggcggagatg ggcgcgctcg gtctgctcgg cgtcaccctc gagggatacg gctgcgcggg
     5041 cgcgagctcg gtcgcctacg gcctggtcgc acgcgagatc gagcgcgtcg attccgccta
     5101 ccggtcgtgc ctgtcggtgc aatcgagcct ggtgatgtgg ccgatccacg cctacggcac
     5161 cgaggcgcag aggcggaaat accttcccgg cctcgcccgc ggcgatctga tcggctgctt
     5221 cggcctgacc gagcccgacc acggctccga ccccggcggc atgatcagcc gcgcgcgcgc
     5281 ggccgccggc ggcttccgcc tctcgggcac caagacctgg atcaccaaca gcccgatcgc
     5341 cgacctggcc gtggtctggg ccaaggacga cgacgggcac atccgcggct tcgtgctcga
     5401 gcgcggcatg gacgggcttg cgacgcccaa gatcgagggc aagttctcgc tgcgcgcctc
     5461 acccaccggc cagatcgtca tggacgacgt gctcgtgccg gccgagaacg cctttcccga
     5521 catccgcggg cttgccggcc cgttcggctg cctcaacaac gcccgctacg gcatcgcctg
     5581 gggggcgatg ggcgcggccg agttctgctg gcaggcggcg cgcgattacg tgctcgaccg
     5641 taagcagttc ggccggccgc tggccgccaa ccagctgatc cagaagaagc tcgccgacat
     5701 gcagaccgag atcacgctcg gcctgaccgc ctgcctgcgg ctgggccggc tgatggacgc
     5761 ggaccgcgcg acacccgaga tgatctcgat gttgaagcgc aacaacgccg gcaaggcgct
     5821 cgacgtggcg cgcatggcgc gcgacatgca cggcggcaac ggcatctccg acgagttcca
     5881 cgtcatccgc cacctggtca atctggagac cgtgaacacc tatgagggca cgcacgacat
     5941 ccacgcgctg atcctcggcc gcgcgcagac cggcattcag gcctttaccg gctgattcgg
     6001 cagcgcggaa cggtcagtcg agcaacggcc gcagcagcgc gtaggcgagc atgccagcgc
     6061 tgaccagcag accggcgacg ggcacccaga tcggacaggc gcgcgcgccg ggcggcagcg
     6121 gatcgcgccg cttgatgcgg atca
//
LOCUS       DASWHU010000267         8218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_139058, whole genome shotgun sequence.
ACCESSION   DASWHU010000267 DASWHU010000000
VERSION     DASWHU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139058"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VGB88_09850"
     CDS             <1..561
                     /locus_tag="VGB88_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9647791.1"
                     /translation="AEARRRAARTVLDQLFECLCAWLAPALAFTAEEAWLERNPSGDG
                     SVHLRTFPELPESWRDDALADKWRRVRAIRRVVTGALEVARRDKRIGASLQAHPELFV
                     TAADAELLAGLDLAELAITSDLTLTVGAGPKDAWRAADEPAVAVAVRLAAGAKCQRCW
                     RVLPEVAADTGLCRRCDAVVAAQAAA"
     gene            564..1049
                     /gene="lspA"
                     /locus_tag="VGB88_09855"
     CDS             564..1049
                     /gene="lspA"
                     /locus_tag="VGB88_09855"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9647792.1"
                     /translation="MLRLGLAVALGVLVLDQALKAVMLGLLPEPGSVRAVTGFFNLVH
                     VRNTGVSFGMLQSDSRLGPWLLAGFAIVVSAALGVWLARAPSRLLAVALGLTIGGALG
                     NVIDRVRFGAVFDFLDLHAFGWHWPAFNLADSAVTVGVAGLLLSSLLAPARGTRVKGP
                     Q"
     gene            1063..1638
                     /locus_tag="VGB88_09860"
     CDS             1063..1638
                     /locus_tag="VGB88_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEX9647793.1"
                     /translation="MTVNATQRAGVLAVAILSLALAGCGQTFKKSLGLGKQVPDEYQV
                     VENAPLSLPPDFHLRPPAPGAPRPQELDVRRQAEEILLGPRQAASGQAELRSAGELAF
                     LNMAGATEVDPGIRQIINRESGVLAAEDEGFVERLMFWQKPPAEGVSIDPVAESERLR
                     QNAEAGRPVTYGPTPVIARKEQAPLEGLSPF"
     gene            1807..3165
                     /locus_tag="VGB88_09865"
     CDS             1807..3165
                     /locus_tag="VGB88_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9647794.1"
                     /translation="MSRARAAAAAVLWLAAALLAPPALAEPGATFGAETFMLDNGMQV
                     VVIPNQRAPVIHHSVWYRVGAADGPRGKSGIAHMLEHLMFKGTEQVPAGEFSKTIARN
                     GGNDNAFTSQDYTGYFQNVSPERLVMVMEMEADRMVNLSFAENDFLTERDVVAEERRS
                     RTGNQASALFAEQVNASQYLAHPYRLPIIGWDQEIQDYTYDDAVDFYRSYYAPNNAIL
                     IVAGDVTAGTLRPLAERTYGRIPARVVAARARPVEPPQLAARRVLMRDARVADPEWMR
                     SYLVPSYHADPDGQAVAIDVLANVLGGGITGRLYQRLVVDRQVANSAGSFYDGLAYDR
                     SRLIVYVRPRPGQAIEEVEAAVDEVLAEVMDRAVDADELARAKFGMRADAVYARDQLA
                     SVARIFGVALTAGLSVADVEGWPELVESVTAEQVQAAARAVLVPARSVTGVLLPETKE
                     AL"
     gene            3168..4517
                     /locus_tag="VGB88_09870"
     CDS             3168..4517
                     /locus_tag="VGB88_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9647795.1"
                     /translation="MARLASCVAGAALLLLVLLAPLRARAVEVERVVSPQGIEAWLVE
                     AHAAPFLTMQFAFRGGTKLDPEGREGLAEFVSALFNEGAGDMDARAFQNALDDNAVRM
                     SFDAGIDELDGTLQTLTERQDRAFELLALALNAPRFDADAVERVRAQQLAGLERDAKD
                     PRAMAEKLWYRAAFADHPYARPPEGAPESVRAITVADLRSWVAARLARDVLAIGVAGD
                     IDAATLGPLLDRVFAGLPAHAAPASVAKTVPDAAGEVLVERMPQPQSLVMWGQRGLAR
                     DDPDYYVAYVMNHILGGGGFTSRLTEEVREKRGLAYGVYSYLLPLDDAPLLMGGVATQ
                     NERVAESLDIVRAEYARLRDGGVSAAELADAKTYITGSFALSLDSNREIAGRLVGMQT
                     ADLGIDYLDRRNGLIEAVTVADVGRVAHALLAPERFTVVVVGDPAGIHPTAAAGGAE"
     gene            4617..5189
                     /locus_tag="VGB88_09875"
     CDS             4617..5189
                     /locus_tag="VGB88_09875"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9647796.1"
                     /translation="MRDSRITVLAALAIVLFSRQYWPDSTLPHELFELAGYALIAACV
                     LGRLYTTAFLGGFKNRQLIDCGPFSVMRNPLYFFSLLGVTGAALMSNHPLVMIGLPAA
                     FLAILMRQIRREERFLATRFGDDYRRYVETVPRLWPRLAGYRAPDSIAVSTRVLASAV
                     ADAVWWFVPLPLIELIEYAQQQGYLPIVFG"
     gene            5242..6540
                     /locus_tag="VGB88_09880"
     CDS             5242..6540
                     /locus_tag="VGB88_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX9647797.1"
                     /translation="MPYDHDISACFAARIGDGGLDEAEFEAALARTRPALARLRAWHA
                     DGSLPLLALPARSDDLAVLQPLIAEAGAKFDRLVVLGTGGSSLGGRTLAALAPQAAGR
                     LHFADNIDPDGFEHLLGGLDLHRTLFAVISKSGGTAETMTQFLIAFEAVRAAVGEAGA
                     RGCFLLVTEPGDNPLRRLGARHKLALFDHDPGLGGRFSVLSLVGMIPALFAGVDALAV
                     RKGAGQVLARTLEARDAAASEPARGAAVQVALAEARGVATTVLMPYCDRLGDFAMWFR
                     QLWAESLGKGGKGTTPVRAIGAVDQHSQLQLYLDGPRDKLFTLVLLDQAGRGRRVEPE
                     LVAGEPALGYLSGRTMGDLFAAEQRATWDTLAARGRPVRVVRLDRLDARALGALLMHF
                     MLETIIAAELWGVDPFDQPAVEDGKRRARQSLAGAAEVPA"
     gene            6537..>8218
                     /gene="mutL"
                     /locus_tag="VGB88_09885"
     CDS             6537..>8218
                     /gene="mutL"
                     /locus_tag="VGB88_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX9647798.1"
                     /translation="MSALRRLPESVVNRIAAGEVVERPANAVKELVENAVDAGARRIE
                     VTLRDGGKSLIAVADDGCGIAAGELALAVERHVTSKLAGDDLSDIRSLGFRGEALPAI
                     GAVSRMTLASRPAGAESAWAIEVAGGRIGAPHPLAGAPGTRVEVRDLFFATPARLKFL
                     KGADAETGACVDAVARIALARPEIAFTVHADGRKRLAADAAQGELLDARRARLAAVMG
                     ESFAANAVPVAAARDGVRIDGFAGLPTYTRAQARQLYLFVNGRPVRDRLLHGAVRAAY
                     RDLLARDRHPVVALFLSLEAAAVDVNVHPAKAEVRFRDAGTVRGLIVSALRHALDAAG
                     YRASTTVAHAALAALTPHAGVPGLFPQPGRAPAAALAEAAAGFQAPLAGTPPAGRVEA
                     GEDPGAESLAEAYPLGAARGQLHETYIVAQTTAGIVIVDQHAAHERLVLERMRRALAS
                     HGVARQMLLIPEVVELDPALAAGLAARAGELAELGLAVEAFGDGAVVVREVPALLGQA
                     DISGLVRDLADDLAEMDSALTLKERLDHVCATMACHGSVRAGRRLTVAEMNAL"
BASE COUNT         1015 a         3048 c         3004 g         1151 t
ORIGIN      
        1 gcggaggcgc gccggcgcgc cgcgcgcacg gtgctcgacc agctgttcga gtgcttgtgc
       61 gcgtggctcg ccccggcgct cgccttcacc gccgaggagg cgtggctcga gcgcaacccc
      121 tcgggcgacg gcagcgtcca cctccgcacc ttccccgagc tgcccgagtc ctggcgcgac
      181 gacgcgctcg cggacaagtg gcggcgggtg cgcgcgatcc ggcgcgtggt caccggcgcg
      241 ctcgaggtcg cccggcgcga caagcgcatc ggcgcgagcc tgcaggcgca tcccgagctg
      301 ttcgtcaccg ccgccgacgc cgagctgctg gcgggcctcg acctcgccga gctcgcgatc
      361 acctcggacc tcacgctcac tgtcggcgcg ggccccaagg acgcatggcg ggcggcggac
      421 gagccggcgg tggcggtggc ggtgcggctc gccgcgggcg ccaagtgcca gcgctgctgg
      481 cgggtgctgc ccgaggtcgc cgccgacacc ggcctgtgcc ggcgctgcga cgccgtggtc
      541 gcggcacagg ccgcggcctg accatgctga ggctcggcct cgcggtcgcg ctcggcgtgc
      601 tggtgctcga ccaggcgctc aaggcggtga tgctcggtct cttgcccgag cccggctcgg
      661 tgcgcgcagt gaccggcttc ttcaacctgg tccatgtgcg caataccggc gtcagcttcg
      721 gcatgttgca gtcggactcg cgcctcgggc cgtggctgct tgccggcttc gcgatcgtgg
      781 tgtccgcggc gctcggcgtg tggctcgcgc gcgcgccctc gcggctgctc gcggtcgcgc
      841 tcgggctgac catcggcggc gcgctcggca acgtcatcga ccgggtgcgc ttcggcgcgg
      901 tgttcgactt cctcgacctg catgccttcg gctggcactg gccggcgttc aacctggcgg
      961 actcggcggt cacggtcggg gtggcgggcc tgctgctcag ctcgttgctc gcgccggcgc
     1021 gaggcactag agtgaaggga ccgcaatgag gcgaggcgaa caatgacggt caacgcaacg
     1081 cagcgggcag gcgtgctggc agtggcgatc ctgagcctgg cgctcgccgg ctgcggccaa
     1141 accttcaaga agagcctggg cctcggcaag caggtccccg acgagtacca ggtggtcgag
     1201 aatgcgccgc tgtcgctgcc gccggacttc cacctgcggc cgccggcacc cggcgcgccg
     1261 cgcccgcagg agctcgacgt gcgccgccag gccgaggaga tcctgctcgg cccgcgccag
     1321 gccgcgagcg gccaggccga gctgcgctcg gcgggcgagc tcgcgttcct caacatggcc
     1381 ggcgcgaccg aggtcgaccc cggcatccgc cagatcatca accgcgagag cggggtgctg
     1441 gcggccgagg acgagggctt cgtcgagcgg ctgatgttct ggcagaagcc gccggcggag
     1501 ggcgtgtcga tcgacccggt ggcggagtcc gagcgcctgc gccagaacgc cgaggccggc
     1561 cggccggtga cctatggccc gacgccggtg attgcgcgca aggaacaggc gccgctcgag
     1621 ggcctgagcc cgttctgagc ggcgcgcagg gccttgtatc ggtgcggcgc aagtccaagt
     1681 tggacaaatg cgggctgccg cgggcactgc cgcgtccggc gcgtatcgac ggcaggaggc
     1741 gtgggccatg tggcggaacg gggcgcggcg cgcagtgacc gggacggcat ccgggagcac
     1801 gggccggtga gccgtgcgcg tgcggccgcc gccgccgtcc tgtggctggc ggcggcgctg
     1861 ctggcgccgc cggcgctggc cgagcccggc gccacgttcg gcgccgagac cttcatgctc
     1921 gacaacggca tgcaggtggt ggtgatcccc aaccagcgcg cgccggtgat ccaccattcg
     1981 gtgtggtatc gcgtcggcgc cgccgacggc ccgcgcggca agtcgggcat cgcccacatg
     2041 ctcgagcacc tgatgttcaa gggcaccgag caggttccgg ccggcgagtt ctcgaagacc
     2101 atcgcccgca acggcggcaa cgacaacgcg ttcaccagcc aggactacac cggctacttc
     2161 cagaacgtct ccccggagcg gctcgtgatg gtgatggaga tggaggccga ccgcatggtc
     2221 aacctgtcct tcgccgagaa cgacttcctc accgagcgcg acgtggtggc cgaggaacgg
     2281 cgcagccgca ccggcaacca ggcgagcgcc ctgttcgccg agcaggtcaa cgcgagccag
     2341 tacctggccc atccctaccg gctgccgatc atcggctggg accaggagat ccaggactac
     2401 acctacgacg acgcggtcga cttctatcgc agctattacg cccccaacaa cgccatcctg
     2461 atcgtcgccg gcgacgtcac cgcggggacg ttgaggccgc tcgccgaacg cacctatggc
     2521 cgcatcccgg cgcgcgtcgt ggcagcgcgg gcgcggccgg tggagccgcc gcagctcgcc
     2581 gcgcgccggg tgctgatgcg ggacgcccgc gtcgccgatc ccgaatggat gcgcagctat
     2641 ctggtgccga gctatcacgc cgacccggac ggccaggcgg tcgcgatcga cgtgctcgcc
     2701 aacgtgctgg gcggcggcat caccggccgg ctctatcagc ggctggtggt cgaccgccag
     2761 gtcgccaact cggccgggtc cttctacgac gggctcgcct acgaccgctc gcggctgatc
     2821 gtctatgtgc gcccgcgccc cgggcaggcg atcgaggagg tcgaggcggc ggtcgacgag
     2881 gtgctggccg aggtcatgga ccgggccgtg gacgcggacg agctcgcgcg cgccaagttc
     2941 ggcatgcgtg ccgatgcggt ctatgcccgt gaccagctcg cctcggtggc acgcatcttc
     3001 ggcgtcgcgc tgaccgcggg cctttcggtc gccgacgtcg agggctggcc cgagctcgtc
     3061 gaatcggtga ccgcggagca ggtccaggcg gcggcgcgcg cggtgctggt gccggcgcgc
     3121 tcggtcaccg gcgtgctgtt gccggaaacc aaagaggcgc tctagcgatg gcgcgcctcg
     3181 cctcgtgcgt cgccggcgcc gcgctgctgc tgctggtcct gctcgcaccg ctgcgcgcgc
     3241 gcgcggtcga ggtcgagcgc gtggtcagcc cccagggcat cgaggcgtgg ctggtcgagg
     3301 cgcacgcggc gccgttcctg accatgcagt tcgccttccg aggcggcacc aagctcgacc
     3361 ccgagggcag ggaggggctg gcggagttcg tctcggcgct gttcaacgag ggcgccggcg
     3421 acatggacgc gcgcgcgttc cagaacgcgc tcgacgacaa tgccgtgcgc atgagcttcg
     3481 acgccggcat cgacgagctc gacggcacgc tgcagaccct gaccgagagg caggaccggg
     3541 ccttcgagct tctggcgctg gcgctcaacg cgccgcgctt cgacgccgac gccgtcgagc
     3601 gggtgcgcgc gcagcagctc gccgggctcg agcgcgacgc caaggacccg cgcgcgatgg
     3661 ccgagaagct gtggtaccgc gccgccttcg ccgaccatcc ttacgcccgc ccgcccgaag
     3721 gcgcgcccga gtcggtgcgt gcgatcacgg tcgcggacct gcgctcctgg gtcgcggcgc
     3781 ggctcgcccg cgacgtgctc gcaatcggcg tcgccggcga catcgatgcc gccaccctcg
     3841 gcccgctcct cgaccgggtg ttcgccggcc tgcctgcgca cgcggcgccg gcgtccgtcg
     3901 ccaagaccgt gcccgacgcc gccggcgagg tgctggtcga gcgcatgccc cagccgcaga
     3961 gcctggtgat gtggggccag cgcggcctcg cccgcgacga ccccgattac tacgtcgcct
     4021 atgtcatgaa ccacatcctc ggcggcggcg gcttcacctc gcgcctgacc gaggaggtgc
     4081 gcgagaagcg cggcctcgcc tatggcgtct acagctacct gttgccgctc gacgacgcgc
     4141 cgttgctgat gggcggggtg gccacccaga acgagcgggt cgccgaatcg ctcgacatcg
     4201 tgcgcgccga gtatgcccgg ctgcgcgacg gcggcgtgtc ggccgcggag ctcgccgacg
     4261 ccaagaccta catcaccggc tcgttcgccc tgtcgctcga ttcgaaccgc gagatcgcgg
     4321 ggcgtctggt cggcatgcag acggccgatc tcggcatcga ctatctcgac cgccgcaacg
     4381 ggctgatcga ggcggtcacg gtcgcggacg tgggccgcgt cgcgcacgcc ctgctcgcgc
     4441 ccgagcggtt caccgtcgtc gtggtcggcg acccggccgg catccatccc accgccgcgg
     4501 cgggcggcgc ggagtaggca acgccggtac cggccgcgcg cgaccacgac cgcctcgacc
     4561 accgcctgct cctgctgccg cgaccgcgaa tggcaagaga cctcaaccag tcacgcatgc
     4621 gcgactcgcg gatcaccgtc ctggccgcgc tcgccatcgt cctgttctcg cgccagtact
     4681 ggcccgacag caccctgcct cacgagctgt tcgagctcgc cggctacgca ctgatcgcgg
     4741 cgtgcgtgct cgggcgcctg tacaccaccg cgttcctggg cgggttcaag aaccgccagc
     4801 tgatcgactg cggcccgttc tcggtgatgc gcaacccgct ctatttcttc tccctgctcg
     4861 gcgtgaccgg cgcggcgctg atgtccaacc atccgctggt gatgatcggg ctgccggcgg
     4921 cgttcctggc catcttgatg cgccagatcc ggcgcgagga gcggttcctc gcgaccaggt
     4981 tcggcgacga ctaccgacgc tatgtggaga cggtgccgag gctgtggccg aggctcgccg
     5041 gctatcgcgc cccggactcg atcgcggtct cgacgcgcgt ccttgccagc gcggtcgccg
     5101 acgcggtgtg gtggttcgtg ccgctgccgc tgatcgagct gatcgagtac gcgcagcaac
     5161 agggctacct gccgatcgtc ttcggctagc gcgccgcggc cgcgctttcc gcacccggtc
     5221 gcctctgcta ggttgcgggc ggtgccctac gaccacgaca tctcggcctg cttcgccgca
     5281 cgcatcggcg atggcgggct cgatgaggcg gagttcgagg ccgcgctcgc ccgcacccgg
     5341 cccgcgctgg cgcgcctgcg cgcctggcac gcggacggca gcttgccgct gctggcgctg
     5401 ccggcgcgca gcgacgacct tgccgtgctg cagccgctga tcgccgaggc gggagccaag
     5461 ttcgaccgcc tggtggtgct cggcaccggc ggctcctcgc tcggcgggcg gaccctcgcc
     5521 gcgctcgcgc cgcaagctgc cggccgcctc cacttcgccg acaacatcga ccccgacggc
     5581 ttcgagcact tgcttggcgg cctcgacctc caccgcaccc tgttcgcggt gatcagcaag
     5641 tcgggcggca ccgccgagac catgacccag ttcctgatcg cgttcgaggc ggtgcgcgcg
     5701 gcggtgggcg aggcgggcgc gcgcggctgc ttcctgctgg tcaccgagcc cggcgacaat
     5761 ccgctcaggc gcctcggcgc acgccacaag ctggcactgt tcgaccacga cccgggcctc
     5821 ggcgggcgct tctcggtgct gtcgctggtc ggcatgattc cggcgctgtt cgcaggcgtc
     5881 gatgcgctcg ccgtgcgcaa gggcgccggg caggtgctcg cgcgcaccct cgaggcccgc
     5941 gacgcggcgg catccgagcc cgcgcggggc gccgccgtcc aggtcgcgct cgccgaggcg
     6001 cgcggcgtcg ccaccaccgt gctgatgccc tattgcgacc ggctcggcga cttcgccatg
     6061 tggttccgcc agctgtgggc ggaatcgctc ggcaagggcg gcaagggcac cacgccggtg
     6121 cgcgcgatcg gcgcggtcga ccagcacagc cagctccagc tctatctcga cggcccgcgc
     6181 gacaagctgt tcacgctcgt cctgctcgac caagccgggc gcgggcgccg ggtcgagccc
     6241 gagctggtcg cgggcgagcc ggcgctcggc tacctgtcgg ggcggaccat gggcgatttg
     6301 ttcgcggccg agcagcgcgc gacctgggac acgctcgccg cgcgcggccg gccggtgcgc
     6361 gtggtccgcc tcgaccggct cgatgcgcgc gcgctcggcg cgctgctgat gcacttcatg
     6421 ctcgagacca tcatcgccgc cgagctgtgg ggggtcgatc cgttcgacca gccggcggtc
     6481 gaggacggca agcggcgcgc ccgccaaagc ctcgccggcg cggccgaggt gccggcgtga
     6541 gcgcgctccg ccgcctgccc gagtcggtgg tcaaccggat cgccgccggc gaggtggtcg
     6601 agcggccggc gaacgcggtc aaggagctgg tcgagaacgc ggtcgacgcc ggcgcgcgcc
     6661 gcatcgaggt gaccttgcgc gacggcggca agagcctgat cgcggtcgcc gacgacggct
     6721 gcggcatcgc cgcgggcgag ctcgcgctgg cggtcgagcg ccacgtcacc tcgaagctcg
     6781 ccggcgacga tctttccgac atccggagcc tcggcttccg cggcgaggcg ctgcccgcga
     6841 tcggtgcggt cagccgcatg acgctcgcct cgcgccccgc cggcgccgag tcggcgtggg
     6901 cgatcgaggt cgcgggcggg cggatcggcg cgccgcaccc gctggccggc gcccccggca
     6961 cccgggtcga ggtgcgcgac ctgttcttcg ccaccccggc ccggctcaag ttcctcaagg
     7021 gcgccgacgc cgagaccggc gcctgcgtcg acgcggtggc gcggatcgcg ctggcgcggc
     7081 ccgagatcgc gttcaccgtc catgccgacg gccgcaagcg gctcgccgcc gatgccgccc
     7141 agggcgagct gttggacgcg cgccgcgcgc gcctcgccgc ggtgatgggc gaatcgttcg
     7201 cggccaacgc ggtgccggtc gccgccgcgc gcgacggcgt gcgcatcgac ggcttcgccg
     7261 ggctgccgac ctacacccgc gcccaggccc gccagctcta tctgttcgtc aacggccggc
     7321 cggtgcgcga ccggctgctg cacggcgccg tgcgcgccgc gtaccgggac ctgctcgccc
     7381 gcgaccgcca cccggtggtg gcgctgtttc tctcgctgga ggcggcggcg gtcgacgtga
     7441 acgtccaccc ggccaaggcc gaggtgcgct tccgcgacgc aggcaccgtg cgcgggctga
     7501 tcgtcagcgc gctccgccac gcgctcgacg ccgccggcta ccgcgcctcg accacggtgg
     7561 cgcatgcggc gctggctgcg ctcacgcccc atgccggcgt gccgggacta tttccccagc
     7621 ccgggcgcgc gccggcggcg gccttggccg aggccgcggc gggattccag gcgccgcttg
     7681 ccggcacccc gcccgccggg cgcgtcgagg cgggcgagga cccgggcgcc gagtcgctcg
     7741 ccgaggccta tccgctgggc gccgcgcgcg gccagctgca cgagacctac atcgtcgccc
     7801 agaccacggc cggaatcgtc atcgtcgacc agcacgccgc ccacgagcgc ctggtgctgg
     7861 agcgcatgcg ccgcgcgctc gcgtcgcacg gcgtcgcccg ccagatgctg ctgatccccg
     7921 aggtggtcga gctcgatccg gcgctcgccg ccggcctcgc ggcgcgcgcc ggcgagctcg
     7981 ccgagctcgg cctggcggtc gaggcgttcg gcgacggcgc ggtggtggtg cgcgaggtgc
     8041 cggccctgct cggccaggcc gacatttccg gcctggtgcg cgacctcgcc gacgacctcg
     8101 ccgagatgga cagcgcgctc accttgaagg agcgtctcga ccacgtctgt gcgaccatgg
     8161 cctgccacgg cagcgtgcgc gccggtcgcc ggctgacggt cgccgagatg aacgcgct
//
LOCUS       DASWHU010000268        12707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1250911, whole genome shotgun sequence.
ACCESSION   DASWHU010000268 DASWHU010000000
VERSION     DASWHU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12707
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250911"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VGB88_09890"
     CDS             complement(<1..321)
                     /locus_tag="VGB88_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9647799.1"
                     /translation="MVADTSALMAILQGEAEASIFLELLDRATVRLVSAVSVLESGLL
                     AETRKGELGGIELDSLLRELDLAVVAFDGEQAEIARSAFRRFGKGRHPAGLNFGDCAA
                     YALAR"
     gene            complement(321..575)
                     /locus_tag="VGB88_09895"
     CDS             complement(321..575)
                     /locus_tag="VGB88_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9647800.1"
                     /translation="MALNIKHPEADRLARELARRQGKTITEVVIHSLESELAREKKRL
                     RTPGLAERVAAIARRYAALPARDTRTDDEILGYDEHGIPT"
     gene            730..996
                     /locus_tag="VGB88_09900"
     CDS             730..996
                     /locus_tag="VGB88_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEX9647801.1"
                     /translation="MAKTDTIRARVEPELKRQAESVLDKLGLSPAEAIRLFYRQVVLR
                     RGLPFAVEIPNAETRAAMRQALEGEDLTEWPDLDTLQRAHNGAD"
     gene            1197..1379
                     /locus_tag="VGB88_09905"
     CDS             1197..1379
                     /locus_tag="VGB88_09905"
                     /inference="COORDINATES: protein motif:HMM:NF027074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEX9647802.1"
                     /translation="MVERLLEAKPLDRRHRAHALSGEWASLHECHVEPDWLLVWRQSD
                     DELVLVRTGTHADLFE"
     gene            1486..2850
                     /gene="ffh"
                     /locus_tag="VGB88_09910"
     CDS             1486..2850
                     /gene="ffh"
                     /locus_tag="VGB88_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9647803.1"
                     /translation="MFDSLADRLGDIFDRLRRRGALGEAEVGAALREVRVALLEADVA
                     LPVVKEFIDKVRARALGAEVLRSVTPGQMVVKIVHDHLVEMLGAEPAPLALAGNPPVA
                     VMMVGLQGSGKTTTAAKLALRLKSRERKKVLLASLDVQRPAAQEQLAVLAGQAGIEAL
                     PQVAGQPPVDIARRAMEAARLGGYDVVVLDTAGRLHVDEALMAELAAVRAATLPHEVL
                     LVADALTGQDAVNIARTFKERAQITGIVLTRIDGDARGGAALSARAVTGCPIKLVGTG
                     EKLDALDVFHPDRIAQRILGMGDVVSLVEKAAESIAEDEAEKLAAKLGQGRFDLDDLA
                     GQLRQMRRMGGIEGLMALLPGVAKVKDQLARANIDQSVIRRQEAIISSMTAAERRNAK
                     LIGASRKRRIAAGSGTTVQDVNRLLKQFKDMGLMMKRVRKLGTKGLARHGLAGALPPG
                     FPTR"
     gene            2914..3495
                     /gene="rpsP"
                     /locus_tag="VGB88_09915"
     CDS             2914..3495
                     /gene="rpsP"
                     /locus_tag="VGB88_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9647804.1"
                     /translation="MALRIRLARAGAKKRPHYRIVVADSRKPRDGRFLEKLGTYSPLL
                     PKDSPERVRLNAERVKYWLERGALPSDRVARMLGGAEVIAMPAARQNPIKARPRKKAQ
                     ERLAAAESAAAAAAAAPAPAVTPAPAAETPAPAPAAETPAPAAETPAPAAETTAPAAE
                     TTAPAAETTGPAAETTGPAPAAGDSGEAGAEPT"
     gene            3510..4052
                     /gene="rimM"
                     /locus_tag="VGB88_09920"
     CDS             3510..4052
                     /gene="rimM"
                     /locus_tag="VGB88_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943933.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9647805.1"
                     /translation="MAARGETGAGARILAGVISGAHGVRGAVRIKPYIADADADSLFK
                     QMCDETGRVLGLHVVAARARDVIATLDGVRDRDAAQALKGTRLYLPRTALPAPGAEEY
                     YHADLIGLAVERTSGEAVGKVSAVHDFGAGAVIEIATAAGAVMVPFTRAAVPVVDLAH
                     GRLVIEPPPGLPGFDRDAGE"
     gene            4049..4771
                     /gene="trmD"
                     /locus_tag="VGB88_09925"
     CDS             4049..4771
                     /gene="trmD"
                     /locus_tag="VGB88_09925"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9647806.1"
                     /translation="MTDTRPWTARVLTLFPDMFPGPLGHSLAGRALADGIWTLEVVDI
                     RAFARDKHRAVDDAPFGGGPGMVMRPDVLAAAIDSLAPPAGGRAARLCLTPRGVPLTQ
                     ARVRALSGTGEVVLVCGRFEGVDQRVIEARGLEEVSVGDFVLSGGEIAAMAVIDACVR
                     LLPGVVGDAAGLEEESFEAGLLEYPHYTRPQTWEGRTVPDVLLSGHHERIRAWRQAEA
                     ETLTRRRRPDLWRRARRDGGGD"
     gene            4811..5227
                     /gene="rplS"
                     /locus_tag="VGB88_09930"
     CDS             4811..5227
                     /gene="rplS"
                     /locus_tag="VGB88_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9647807.1"
                     /translation="MNIIEQLEQEQIARLGEGREIPRFAPGDTVRVGVRVVEGTRSRL
                     QAFEGVCIARSNKGINSNFTVRKISYGEGVERTFPLYSPLVEKIELVRRGDVRRAKLY
                     YLRGLTGKAARITEKREERGAQAGNAEAADGGDTGS"
     gene            5251..6657
                     /gene="leuC"
                     /locus_tag="VGB88_09935"
     CDS             5251..6657
                     /gene="leuC"
                     /locus_tag="VGB88_09935"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015229778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9647808.1"
                     /translation="MAAPRTLFDKIWDGHLVHSQEDGTALIYIDRHLVHEVTSPQAFE
                     GLRQAGRRPRRPAAALAVADHNVPTTDRSAGIADETSRIQVETLEANCAEFGVPYFEV
                     SDLRQGIVHIIGPEQGFTLPGTTIVCGDSHTATHGAFGALAVGIGTSEVEHVLATQTL
                     IQRRPRNMRITVAGALPRGVTAKDVILSIIGRIGTAGGTGHVIEYAGAAIRALSMEGR
                     MTVCNMSIEAGARAGLIAPDETTFAYLKGRPMAPKAGRWEQAVAYWRSLPSDDGAAFD
                     AELRMDAGELVPHVTWGTSPEDVLPITGRVPDPAAVADADRRRSMERALAYMALTPGT
                     PLAAVAIDRVFIGSCTNGRIEDLRAAAEVARGRRVAQGVGAMVVPGSGLVKHQAEDEG
                     LDRIFTDAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGRGGRTHLMSPA
                     MAAAAAVTGRLCDVRALD"
     gene            6685..7296
                     /gene="leuD"
                     /locus_tag="VGB88_09940"
     CDS             6685..7296
                     /gene="leuD"
                     /locus_tag="VGB88_09940"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9647809.1"
                     /translation="MEPFVKLTGIAAPLRMINVDTDMIIPKQHLKTIRRTGLGRFLFD
                     ELRFTPDGAERRDFVLNRAPYRGAQILIAGENFGCGSSREHAPWALLDYGIRCVIAPS
                     FADIFYNNCFKNGVLPVRLPKAVVESLMDQAEQGANARVTVDLEACEVRAPDGTAHSF
                     EIDAFRRHCLLEGLDDIALTLQKSAEIANFEAGQTRVQPWLYG"
     gene            7500..8612
                     /gene="leuB"
                     /locus_tag="VGB88_09945"
     CDS             7500..8612
                     /gene="leuB"
                     /locus_tag="VGB88_09945"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX9647810.1"
                     /translation="MARNKRLLLLPGDGIGPEVMAEVRRVMAWFDKRRALGFEVEDGL
                     VGGISYDRHGTPLTDEVMALALEVDAVLFGAVGGPKWDHLDFSVKPERGLLRLRKEMD
                     LFANLRPAVLFEPLIEASTLKPEVVRGLDMMIVRELTGGIYFGSPRGIERLADGSRRG
                     VNTEVYSEAEIERVGRVALELARKRRKLVHSVDKANVMESGVLWREVMTALHKAEYAD
                     VELRHMYADNAAMQLVRDPRQFDVMVAGNLFADILSDCAAMLTGSLGMLPSASLGAPD
                     GAGRRRALYEPVHGSAPDIAGQGRANPIAMLMSYAMMLRYSFDLADEADLVERAITGV
                     LDGGLRTADIMQPGKARVSTEVMGEAVVSELDKLAA"
     gene            8775..9788
                     /locus_tag="VGB88_09950"
     CDS             8775..9788
                     /locus_tag="VGB88_09950"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9647811.1"
                     /translation="MGYRVAVVGATGNVGREILNIMAERHFPADEVVAVASAASKGNQ
                     VSYGEDGVLEVRDLAKFDFAGFDLGLFSPGAKVSAEHAPRAAAAGCIVIDNTSQFRMD
                     ADVPLVVPEVNPDALIGFRERNIIANPNCSTIQMVVALKPLHDLARIRRVVVATYQSV
                     SGAGKKAMDELYDQTRKIFFHEMKEPEVFTRRIAFNVIPHIDAFQPDGSTREELKMAR
                     ETQKILDPAILVTATCVRVPVFVGHAEAINVEFETPLDVAEARAALADAPNVSLIDDP
                     DENEYVTPIECVGDYEVFVSRVRQDTTVPYGLNLWVVSDNLRKGAAFNAVQIAEMLVA
                     EYL"
     gene            9986..11137
                     /locus_tag="VGB88_09955"
     CDS             9986..11137
                     /locus_tag="VGB88_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9647812.1"
                     /translation="MSASGRTPDTDLIHAGEPEPRIDGAVRVPIFQTAMYEVRDDDPR
                     GVRYIRYTNTPNHEVLEKKLAVLEHAEAGLVTGSGMAAISAALLTVLAAGRHLLVLGP
                     VYGGTHQFVTAMLPGLGIAHSMADGRDPDGWAAALKPETAAVYTETITNPLVGVPDHA
                     AVVAFARAHGLVSMIDNTFATPINFRPVEHGYDLVMHSATKYLNGHSDVVAGAVVGPS
                     ELVERCRWTLTNLGGALDPHAAFLVHRGMATLAVRVRHQNASALAVARFLATHARVRW
                     VSYPGLQGHPDHGRARALLDGCGGVLAFELDGGRDAAQRFMARVTIPISAPSLGGVET
                     LLSRPAVTSHARMSADQRAALGISDSLIRCSIGLEGTDDLIADFAQALG"
     gene            complement(11162..11566)
                     /locus_tag="VGB88_09960"
     CDS             complement(11162..11566)
                     /locus_tag="VGB88_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9647813.1"
                     /translation="MLLDTCAAIWLMNGDPMTDTSRQAIRAARIRGHGVYVSPISAWE
                     IATLVAKERIQLAYAPETWFERMLVLPGMRLAGLSPAVLIRSAFLPGKPPTDPADRII
                     AATAREFEFVVVTRDRPLLAYGRAGHLNVRAC"
     gene            complement(11571..11828)
                     /locus_tag="VGB88_09965"
     CDS             complement(11571..11828)
                     /locus_tag="VGB88_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9647814.1"
                     /translation="MAKPLRDETVIGVTAFKAKCLALIDEVARGKTRRVVLTKHGHPV
                     AVLGPAEGKRVELWGAMRGSVRMAPGTDLTQPTGEVWDANA"
     gene            complement(11918..>12707)
                     /gene="pdxY"
                     /locus_tag="VGB88_09970"
     CDS             complement(11918..>12707)
                     /gene="pdxY"
                     /locus_tag="VGB88_09970"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HEX9647815.1"
                     /translation="VGNSAAVFALQRLGVEAWPVHTVQLSNHTGYPDWGGGALGPAHV
                     AAVLDGLERRGAFGAAEAVLTGYVGDAALAAVIASAVDRITAAGARYCCDPVLGNDAR
                     GLFVPEAAARAMAAELLPRADIATPNRFELAWLTGMEVATAADALAAADALAARGPRM
                     VAVTSLPVADKAGIGVLLRTDAGAWQVATLRLQVAANGAGDAFAAMLLARLVRGESPD
                     HALALATASIFAILAASGTGELALVAAQDEIVRPSRGFAAVRLR"
BASE COUNT         1943 a         4475 c         4538 g         1751 t
ORIGIN      
        1 ccgcgcgagc gcgtaggccg cacagtcgcc gaagttgagc ccggcaggat ggcgtccctt
       61 gccgaagcgg cgaaaagccg agcgcgcgat ctcggcctgc tcaccgtcga acgcgaccac
      121 tgcgagatcc agctcgcgca gcaggctgtc gagctcgata ccgcccagct cgcccttgcg
      181 cgtctcggcg agcagacctg actcgagcac ggagaccgcg gaaaccagcc tcaccgtcgc
      241 ccggtcgagc agctccagga agatcgaggc ttcggcctcc ccctggagga tggccatgag
      301 agcggaggtg tcggcgacca tcaggtcgga atgccgtgct cgtcgtagcc caggatctcg
      361 tcgtcggtgc gcgtgtcgcg ggcgggaagt gcggcatagc ggcgggcgat cgcggccacg
      421 cgctccgcca gtcccggcgt tcgaaggcgc ttcttctcgc gcgcaagctc gctctcaagg
      481 gagtggatga cgacctcggt gatcgtcttc ccctgccggc gggcgagctc ccgcgccagc
      541 cggtcggcct cgggatgctt tatgttgagc gccacggttc ttccgtattc ttcctgaccg
      601 actacattct atacgtcgac cacgttcgcg caatacgtta acttagttag tggacgccca
      661 gcgatttccg ggggggcacc gattgttccc caatcgtgct acactgtcgg cgacgggagt
      721 catcaaccca tggccaagac ggataccatt cgtgcccggg tcgagcccga gctcaagagg
      781 caggcggagt cggtgctgga caagctcggg ctgtcaccgg ccgaggcgat ccggctgttt
      841 taccgccagg tcgtgctccg tcgcggcctg ccattcgctg tcgagattcc gaatgccgag
      901 acccgcgcgg cgatgcgcca ggcgctggag ggcgaggacc tgaccgagtg gccagacctc
      961 gacaccctgc aacgcgccca caacggcgca gattgaccgt atcagccatt ggcgcccgaa
     1021 atcccaccgc agtctcagtc aagttgtcgg tgacctagac gcgcggaggc attgcgacgg
     1081 ggcgcgacag ccatgggagc gggccggtga cgcgccgcct caagacgacc aagcggttcg
     1141 aacgcgacct gaagcgcgcc aagcgacgag gcaaaaacct gcagaagctg taggcggtgg
     1201 tcgagcgact gctcgaagcc aagccgctcg accggcggca ccgcgcgcat gcgttgtcgg
     1261 gagagtgggc gagcttgcac gagtgccacg tcgagcctga ctggctgctg gtctggcgcc
     1321 agagcgacga cgagttggtc ctggtccgga ccggcaccca cgccgacttg tttgagtgag
     1381 tctgccgccg gcgcttgact tggggcggga tttggcgtta ggcacgcggc gtccgcggcg
     1441 ggccgccggt tgaggcgccg ccgcccgggt tcctgggaag ctggcatgtt cgactcgctc
     1501 gccgaccgtc tcggcgacat cttcgaccgt ctgcgacggc gcggcgcgct cggcgaggcc
     1561 gaggtgggcg cggcgctgcg cgaggtgcgc gtggcgctgc tcgaggcaga cgtcgcgctg
     1621 ccggtggtca aggagttcat cgacaaggtg cgcgcgcgcg cgctcggcgc cgaggtgctg
     1681 cgctcggtca cgcccggcca gatggtggtc aagatcgtcc acgaccacct ggtcgagatg
     1741 ctgggcgccg agcccgcgcc gctcgccctc gccggcaacc cgccggtggc ggtgatgatg
     1801 gtcggcctgc agggctcggg caagacgacc accgctgcca agctcgcgct gcgcctcaag
     1861 agccgcgagc gcaagaaggt gctgctcgcc tcgctcgacg tccagcgccc ggcggcgcag
     1921 gagcagctcg cggtgctcgc gggccaggcc ggcatcgagg cgctgccgca ggtcgccggc
     1981 cagccgccgg tcgacatcgc caggcgcgcg atggaagcgg ctcggctcgg cggctacgac
     2041 gtggtggtgc tcgacaccgc cggccggctg cacgtcgacg aggcgctgat ggccgagctc
     2101 gctgcggtgc gcgccgccac cctgccgcac gaggtcctcc tggtcgccga cgcgctgacc
     2161 ggccaggacg cggtcaacat cgcgcgcacc ttcaaggagc gggcgcagat cactggcatc
     2221 gtgctcaccc gcatcgacgg cgacgcccgc ggcggcgcgg cgctgagcgc gcgcgcggtc
     2281 accggctgcc cgatcaagct ggtcggcacc ggcgagaagc tggatgcgct cgacgtgttc
     2341 catcccgacc gtatcgccca gcgcatcctc ggcatgggcg acgtcgtctc gctggtcgag
     2401 aaggcggccg agtcgatcgc cgaggacgag gccgagaagc tcgccgccaa gctcgggcag
     2461 ggccgcttcg acctcgacga ccttgccggc cagctccgcc agatgcgccg gatgggcggc
     2521 atcgaggggc tgatggccct gctgccgggg gtcgccaagg tcaaggacca gctggcccga
     2581 gccaatatcg accagtccgt gatccgccgc caggaggcga tcatctcgtc gatgaccgcg
     2641 gccgagcggc gcaacgccaa gctgatcggc gcctcgcgca agcggcgcat cgccgccggc
     2701 tccggcacca cggtccagga cgttaaccgc ctgctcaagc agttcaagga catgggcctg
     2761 atgatgaagc gggtccggaa gctggggacg aagggcctcg cccgccacgg cctcgccggc
     2821 gcgctgccgc cgggcttccc gacccgctga tgtggaccgc ggccacgggt ttggattaaa
     2881 agacgcgacg cgcgtcgtcg gaggttggaa agcatggcgt tgaggattcg tctggccagg
     2941 gccggagcca agaagcgtcc gcactaccgg atcgtggtcg cggattcgcg caagccgcgc
     3001 gacggccgct tcctcgagaa gctcggcacc tacagcccgc tgctgcccaa ggacagcccc
     3061 gagcgcgtgc gcctcaatgc cgagcgggtc aagtactggc tcgagcgcgg cgcgctgccc
     3121 agcgaccggg tcgcgcgcat gctcggcggg gccgaggtca tcgccatgcc ggcggcgcgc
     3181 cagaacccga tcaaggcgcg gccgcggaag aaggcgcagg agcgcctggc cgcggccgag
     3241 agcgcggcag cggcggcagc ggcggcgccg gcgccggccg tgacgcccgc gcccgcggcc
     3301 gagacgcccg cgcccgcgcc cgcggccgag acgcccgcgc ccgcggccga gacgcccgcg
     3361 cccgcggccg agacgactgc gcccgcggcc gagacgactg cgcccgcggc cgagacgact
     3421 gggcccgcgg ccgagacgac tgggcccgcg ccggcggccg gggacagcgg cgaggccggc
     3481 gccgagccga cctaagctcg gcatcgagca tggcggcgcg cggcgagaca ggggccggcg
     3541 cgcgcatcct cgccggcgtg atcagcggcg cccacggcgt gcgcggcgcg gtcaggatca
     3601 agccctacat cgccgatgcg gacgcggact ccttgttcaa acagatgtgc gacgagaccg
     3661 gccgggtcct cggcttgcac gtcgtcgcgg cgcgcgcccg cgacgtgatc gcgacgctgg
     3721 acggggtgcg cgaccgcgac gccgcccagg cgctcaaggg cacccggctg tacctgccgc
     3781 gcaccgcgct gcccgcgccg ggcgccgagg agtactatca cgctgacctg atcgggcttg
     3841 cggtcgagcg caccagcggc gaggcagtgg gcaaagtgag cgcggtccac gacttcggtg
     3901 ccggcgccgt gatcgagatc gcgaccgcag cgggcgcggt gatggttccc ttcacccgcg
     3961 cggcggtgcc ggtggtcgac cttgcgcacg ggcggctggt gatcgagccg ccgccgggcc
     4021 tgcccggctt cgaccgggac gccggcgaat gaccgacacc cggccttgga cggcgcgcgt
     4081 cctgacccta tttcccgaca tgttcccggg cccgctcggc cacagccttg ccgggcgggc
     4141 gctggccgac ggcatctgga cgctggaagt ggtggacatt cgcgccttcg cgcgcgataa
     4201 acaccgcgcc gtcgacgacg cgccgttcgg cggcggaccc ggcatggtga tgcggcccga
     4261 cgtgctggcg gcggcgatcg actcgctggc gccgcccgcg ggcggacgag cggcgcgatt
     4321 gtgcctcacc ccgcgcggcg tgccgctgac ccaggcgcgg gtgcgcgcgc tcagcgggac
     4381 gggcgaggtg gtgctggtgt gcggccggtt cgagggcgtc gatcagcgcg tgatcgaggc
     4441 ccgcgggctc gaggaggtga gcgtcggcga cttcgtgctg tcgggcggcg agatcgccgc
     4501 gatggccgtg atcgacgcct gcgtgcggct gctgccggga gtggtcggcg acgcggccgg
     4561 gctggaggag gagagcttcg aggcgggtct gctcgagtac ccgcactaca cgcgcccgca
     4621 gacgtgggaa ggccgcacgg tgcccgatgt gctgctgtcc ggccaccacg agcgcatccg
     4681 ggcgtggcgg caggccgagg cggagacgct cacccgccgg cgccggccgg acttgtggcg
     4741 ccgcgcccgg cgcgacggcg gcggcgacta gaggatagaa gggcaacagg accgagggcg
     4801 gatggcggcc atgaacatca tcgagcagct cgagcaggag cagatcgcca ggcttggcga
     4861 agggcgcgag atcccgcgct tcgcccccgg cgacaccgtg agggtcggcg tgcgcgtggt
     4921 cgagggcacc cgctcgcgcc tgcaggcgtt cgagggcgtg tgcatcgcgc gctcgaacaa
     4981 gggcatcaac tcgaacttca ccgtgcgcaa gatctcatac ggcgaagggg tggagcgcac
     5041 cttcccgctg tattcgccgc tggtcgagaa gatcgagctg gtgcgccgcg gcgacgtgcg
     5101 ccgggccaag ctctattacc tgcgcgggct caccggcaag gcggcacgca tcaccgagaa
     5161 gcgcgaggag cgcggcgccc aggcgggcaa cgccgaggcc gcggacggcg gcgacaccgg
     5221 ctcgtgagcc cgagcgatcg ccaacccgcc atggccgcgc cgcgcaccct gttcgacaag
     5281 atctgggacg gccacctggt ccacagccag gaggacggca ccgcgctgat ctacatcgac
     5341 cgccatctgg tccacgaggt gaccagcccg caggcgttcg aggggctgcg ccaggccggg
     5401 cgcaggccgc gccggcccgc ggcggcgctc gcggtcgccg accacaacgt gccgaccacc
     5461 gaccgcagcg ccggcatcgc cgacgagacc tcgcgcatcc aggtcgagac cctggaggcg
     5521 aactgcgccg agttcggggt gccctacttc gaggtctccg acctgcgcca gggcatcgtc
     5581 cacatcatcg gacccgagca gggcttcacg ctgcccggca cgaccatcgt ctgcggcgac
     5641 tcgcacaccg ccacccacgg cgcgttcggc gcgctcgccg tcggcatcgg caccagcgag
     5701 gtcgagcacg tgctcgccac ccagaccctg atccagcgcc ggccgaggaa catgcgcatc
     5761 acggtcgcgg gcgcgctgcc gcgcggggtc accgccaagg acgtgatcct ctccatcatc
     5821 gggcgcatcg gcaccgccgg cggcaccggc catgtgatcg aatatgccgg cgcggcgatc
     5881 cgggcgctgt cgatggaagg ccgcatgacg gtgtgcaaca tgtcgatcga ggcgggcgcg
     5941 cgcgccggcc tgatcgcccc cgacgagacc accttcgcct acctgaaggg ccggccgatg
     6001 gcgcctaagg ccgggaggtg ggagcaggcg gtcgcctact ggcgcagctt gccgagcgac
     6061 gacggcgcgg cgttcgacgc cgagctgcgc atggatgcgg gcgagctggt cccgcacgtc
     6121 acctggggca ccagccctga ggacgtgctg ccgatcacgg ggcgcgttcc cgacccggcc
     6181 gcggtggcgg atgcggaccg gcgccggtcg atggagcgcg cgctcgccta catggcgctc
     6241 accccgggca cgccgctcgc cgcggtcgcc atcgatcggg tgttcatcgg cagctgcacc
     6301 aacggccgca tcgaggactt gcgtgccgcc gccgaggtgg ccagggggcg ccgggtcgcc
     6361 caaggcgtcg gcgctatggt cgtgccgggc tccgggctgg tcaagcacca ggccgaggac
     6421 gaagggctcg accgcatctt caccgacgcc ggcttcgagt ggcgcgagcc cggctgctcg
     6481 atgtgcctgg cgatgaacgc cgatcggctg gagcccggcg agcgctgcgc gtcgacctcc
     6541 aaccgcaact tcgagggccg ccaggggcgc ggcgggcgca cccacctgat gagcccggcg
     6601 atggccgcgg ccgcggccgt cacgggccgg ctgtgcgacg tgcgcgcgct cgactaggac
     6661 ttcgccgaac aagggacaag agccatggag ccgttcgtca agctgaccgg gatcgcggcg
     6721 ccgctgcgga tgatcaacgt cgacaccgac atgatcatcc ccaagcagca cctgaagacc
     6781 atccggcgca ccggcctcgg ccgcttcctg ttcgacgagc tcaggttcac gcccgacggc
     6841 gccgagcggc gcgacttcgt gctcaaccgc gcgccctacc ggggcgcgca gatcctgatc
     6901 gcgggcgaga acttcggctg cggctcgagc cgcgagcacg cgccgtgggc gctcttggac
     6961 tacggcatcc gctgcgtgat cgcgccgagc ttcgccgaca tcttctacaa caactgcttc
     7021 aagaacggcg tcctgccggt gaggctgccg aaggcggtgg tcgaatcgct gatggaccag
     7081 gccgagcagg gcgccaatgc gcgcgtcacg gtcgacctcg aggcgtgcga ggtacgtgcg
     7141 cccgacggca cggcgcattc gttcgagatc gatgcgttcc ggcgccactg cctgctcgag
     7201 ggcctcgacg acatcgccct cacgcttcag aagagcgccg agatcgcgaa tttcgaggcc
     7261 ggccagacgc gcgtccagcc gtggctgtac ggctgaggtc tctccttcgc tcgcgccgac
     7321 cctgatcgcc ttgacacggt cccccctcac ccagctacgg gtaaggcgcg gcgtgcgccg
     7381 cgccgaccct gcgcaaccct ctccccgcca cgcgggcgga gagggtaggg tgaggggggc
     7441 tgatcgacgc aaagcggaac atcctttagg tcgctcgcga ctacggtagg ggagtgctga
     7501 tggcccgcaa caagcgactg ctgctcttgc ccggcgacgg catcgggccc gaggtgatgg
     7561 ccgaggtgcg ccgggtgatg gcctggttcg acaagcggcg cgcgctcggc ttcgaggtcg
     7621 aggacggcct ggtcggcggc atctcgtacg accgccacgg caccccgctc accgacgagg
     7681 tcatggcctt ggcgctcgag gtcgacgcgg tgctgttcgg cgccgtcggc gggccgaaat
     7741 gggaccacct cgacttctcg gtcaagcccg agcgcgggct gctgcgccta cgcaaggaga
     7801 tggacctgtt cgccaacctg cgcccggcgg tgctgttcga gccgctgatc gaggcctcga
     7861 ccttgaagcc cgaggtggtc cgcggcctcg acatgatgat cgtgcgcgag ctgaccggcg
     7921 gcatctattt cggtagcccg cgcggcatcg agcggctcgc cgacggcagc cggcgcgggg
     7981 tcaacaccga ggtctactcg gaggccgaga tcgagcgcgt cgggcgggtc gcgctcgagc
     8041 tcgcgcgcaa gcggcgcaag ctggtccact cggtcgacaa ggcgaacgtg atggagtcgg
     8101 gcgtgctctg gcgcgaggtg atgaccgcgc tccacaaggc agagtatgcc gacgtcgagc
     8161 tccgccacat gtacgccgac aacgccgcca tgcagctggt ccgcgatccc cggcagttcg
     8221 acgtcatggt cgccggcaac ctgttcgccg acatcctctc ggactgcgcg gcgatgctga
     8281 ccggatcgct cggcatgctg ccctcggcga gcctcggcgc gcccgacggc gcggggcgcc
     8341 ggcgcgcgct ctatgagccg gtgcacggca gcgcccccga catcgccggc cagggccggg
     8401 cgaacccgat cgcgatgctg atgtcgtacg cgatgatgct gcgctactcg ttcgacctcg
     8461 cggacgaggc ggacctggtc gagcgcgcga tcaccggggt gcttgacggc ggcctgcgca
     8521 cggcggacat catgcagccg ggcaaggcgc gggtgtcgac cgaggtgatg ggcgaggcgg
     8581 tggtcagcga gctcgacaag ctcgccgcat gagcgcgcag cgacgccggc cgtggcgcgc
     8641 gcgctcgctg gcggccgtac tcggccaggc aggacggcca gacctcgcca taggtcttga
     8701 gtttttcggc catcggcgca ccttggcgtc ggcgggcgag cacagcatga ggacagccga
     8761 gggctgaaac gggcatgggc tacagggtag cggtggtcgg cgccaccggc aatgtcgggc
     8821 gcgagatcct caacatcatg gccgagcgcc atttccccgc cgacgaggtg gtggcggtcg
     8881 cctccgccgc ctcgaagggc aaccaggtgt cctacggcga ggacggggtg ctcgaggtgc
     8941 gcgacctggc caagttcgac ttcgcgggct tcgacctcgg cctgttctcg cccggtgcca
     9001 aggtctcggc cgagcacgcg ccgcgcgccg ccgccgccgg ctgcatcgtg atcgacaaca
     9061 cctcgcagtt ccgcatggat gcggacgtgc cgctggtggt gcccgaggtc aacccggacg
     9121 cgctgatcgg cttccgcgag cgcaacatca tcgccaaccc caactgctcg accatccaga
     9181 tggtggtggc gctcaagccg ctgcacgacc tggcgcggat caggcgcgtg gtcgtcgcca
     9241 cctaccagtc ggtgtcgggc gcgggcaaga aggcgatgga cgagctctac gaccagaccc
     9301 gcaagatctt cttccacgag atgaaggagc cggaggtgtt cacgcgccgg atcgcgttca
     9361 acgtgatccc gcacatcgac gccttccagc ccgacggctc gacccgggag gagctcaaga
     9421 tggcgcgcga gacccagaag atcctcgacc ccgcgatcct ggtgaccgcg acctgcgtgc
     9481 gcgtgccggt gttcgtcggc cacgccgagg cgatcaacgt cgagttcgag accccgctcg
     9541 acgtcgcgga ggcgcgcgcg gcgctggcgg acgcgcccaa cgtctcgctg atcgacgacc
     9601 cggacgagaa cgagtacgtg accccgatcg agtgcgtcgg cgactacgag gtgttcgtca
     9661 gccgcgtgcg ccaggacacc acggttccct acggcctcaa cctgtgggtg gtctcggaca
     9721 acctgcgcaa gggcgccgcg ttcaacgccg tccagatcgc cgagatgctg gtcgccgagt
     9781 acctatagga gcgagattcg gaaatgggcg aaacatttgt cgtccttcga gacgctcgct
     9841 tcgctcgctc ctcaggacga ggttttcctc ttttgtttca gcgacctcat cctgcggagg
     9901 ccgcgtcagc ggccgtctcg aaggatgagg ttgttgctga gtcacccgtc tacgacgggc
     9961 ggcactagcg aacggaggcc gagccatgtc cgccagcggc aggacccccg acaccgacct
    10021 gatccacgcc ggcgagcccg agcccaggat cgacggcgcg gtgcgggtgc cgatcttcca
    10081 gaccgcgatg tacgaggtgc gcgatgacga tccgcgcggg gtgcgctaca tccgctacac
    10141 caacaccccc aaccacgagg tgctggagaa gaagctggcg gtgctcgagc acgccgaggc
    10201 cgggctggtc accggcagcg gcatggccgc gatctcggcg gcgctcttga cggtgctcgc
    10261 cgccggccgc cacctcctgg tgctcggccc ggtctacggc ggcacccacc agttcgtcac
    10321 cgccatgctg ccggggctcg gcatcgcgca cagcatggcc gacggccgcg atccggacgg
    10381 ctgggccgcg gcgctcaagc ccgagaccgc ggcggtctac accgagacca tcaccaaccc
    10441 gctggtgggc gtgcccgacc acgccgccgt ggtcgccttc gcccgcgccc acggcctggt
    10501 gtcgatgatc gacaacacct tcgccacccc gatcaacttc cgccccgtcg agcacggcta
    10561 cgacctggtc atgcacagcg cgaccaagta cctgaacggc cattccgacg tggtcgcggg
    10621 cgccgtcgtc gggccgagcg agctggtcga gcgctgccgc tggaccttga ccaacctggg
    10681 cggcgcgctc gacccgcacg cggcgttcct ggtccatcgg ggcatggcga ccttggcggt
    10741 gcgggtgcgc caccagaacg cgagcgcgct cgccgtggcg cgctttctcg ccacccacgc
    10801 acgcgtgcgc tgggtcagct atccggggct gcagggccat cccgaccacg gccgcgcgcg
    10861 cgccctgctc gacggctgcg gcggcgtgct cgcgttcgaa ctcgacggcg gccgcgacgc
    10921 cgcccagcgg ttcatggccc gggtcaccat cccgatctcg gcgccgagcc tgggcggggt
    10981 cgagacgctg ctgtcgcgac cggcggtgac cagccacgcg cgcatgagcg ccgaccagcg
    11041 cgccgcgctc ggcatctcgg actcgctgat ccgctgctcg atcggcctcg aaggcaccga
    11101 cgacctgatc gccgacttcg cccaggccct cggctgatct cgtttccggc cgatagccgg
    11161 atcagcaggc acgcacgttg aggtgcccgg cgcgaccgta ggcgagcaaa gggcggtcgc
    11221 gcgtgaccac gacaaattcg aactcgcgcg cagttgcggc gatgatccgg tcagccggat
    11281 ccgtgggtgg tttcccgggc aggaatgcag agcggatcag gacggccggc gacaagccgg
    11341 caagccgcat acccggcaga accagcattc tttcgaacca agtctcgggc gcataggcga
    11401 gctggattcg ctccttcgca accagcgtcg cgatctccca agcactgatt ggagagacat
    11461 agacgccgtg gccacgaatc ctggctgcgc ggatcgcctg gcggctcgtg tcggtcatcg
    11521 gatcgccatt catcagccaa atcgccgcgc aggtatcgag cagcacgggc tcaggcattt
    11581 gcgtcccaga cctcgccagt cggctgggtc aagtcggtcc cgggggccat gcgcacagat
    11641 ccgcgcattg cgccccataa ctcgacccgc ttaccttcag cgggccccag taccgcgacc
    11701 gggtgaccat gcttggtcag taccacgcgg cgcgtcttgc cgcgtgcgac ctcgtcgatc
    11761 aaggcgaggc attttgcctt gaacgcggtc acgccgatca ctgtttcatc gcgcaggggc
    11821 ttcgccatat caggttctcc aaaatggact ataatttatg gtccataagg gcctaacctg
    11881 acccatttct tatcgcgagg caagggcgga ataggcgtca gcgcagccgc accgcggcga
    11941 aaccgcggct ggggcgcacg atctcgtcct gggcggcgac gagcgcgagc tcgccggtgc
    12001 cgcttgcggc gaggatggcg aagatcgacg ccgtcgccag ggcgagcgca tggtccggcg
    12061 actcgccgcg caccaggcgg gcgagcagca tcgcggcgaa ggcatcgccg gcgccgttcg
    12121 ccgccacctg cagccgcagc gtcgcgacct gccaggcgcc ggcgtcggtg cgcagcaaca
    12181 cgccgatccc ggccttgtcc gcgaccggca gcgaggtcac cgcgaccatg cgcgggccgc
    12241 gcgcggcgag cgcatcggcc gcggccagcg cgtccgcggc cgtcgccacc tccatcccgg
    12301 tcagccaggc gagctcgaac cggttggggg tggcgatgtc ggcgcgcggc agcagctccg
    12361 ccgccatcgc ccgcgctgcc gcctccggca cgaacaggcc gcgcgcatcg ttgccgagca
    12421 ccgggtcgca gcagtagcgc gcgccggcgg cggtgatccg atcaaccgcc gaagcgatca
    12481 ccgcggcgag cgcggcgtcg ccgacatagc cggtgagcac cgcctcggcc gcgccgaacg
    12541 caccccgccg ctcgagcccg tcaagcaccg cggcgacgtg cgcgggcccg agcgcgcccc
    12601 cgccccagtc ggggtagccg gtgtggttcg agagctggac ggtatggacc ggccacgcct
    12661 cgacaccgag gcgttgaagc gcgaacacgg cggcgctgtt gccgaca
//
LOCUS       DASWHU010000269         9500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1639428, whole genome shotgun sequence.
ACCESSION   DASWHU010000269 DASWHU010000000
VERSION     DASWHU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639428"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGB88_09975"
     CDS             complement(<1..551)
                     /locus_tag="VGB88_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9647816.1"
                     /translation="MIAALVVLIAVRLPVALAMGLTGAGGYVALAGWAPLSGYLKTAM
                     VDKFVSYDLAIIPLFILMGQLATRSGVSRSIFDACNAWLGHRRGGLAMASVAGCAAFG
                     AICGSSIATATTMGQVALPEMRRHGTSAALSAGTLAAGGTLGILIPPSIILVIYAVLT
                     EQNIVKLFLAATVPGLLAVLGFMV"
     gene            complement(575..1090)
                     /locus_tag="VGB88_09980"
     CDS             complement(575..1090)
                     /locus_tag="VGB88_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9647817.1"
                     /translation="MAGAPEGALDRAVDGLCRAAAALGGLAIFAIALTVFVSVFGRSF
                     RLLSIPGDFEVVEIGSAAAVFLFFPICQLERAHITIELFTDWLPVSARRGLEAAGELL
                     FALAWAVLVWRLTVGGIEAHGYGDRSMILGVPTWIVYLPAVAALAIAGLVALQSARRL
                     LAAAGRGARPS"
     gene            complement(1094..2155)
                     /locus_tag="VGB88_09985"
     CDS             complement(1094..2155)
                     /locus_tag="VGB88_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647818.1"
                     /translation="MAKGSLRITRRRAAALIAGGVCAPALIGRARAAEVTLKLHHMLP
                     PVSPAHRDILAPWAKRVAEQSGGRIEVQVFAAMQLGGKPAQLIDQVRDGIADISWALP
                     VYNPGRYPLAETATLPFMVTTAEATSVALHKFMAAYGGGEFADVHVLAFHVHAPGKFH
                     MREKPITGAADLAGLRIRAPNQVFGRALGILGAEPVFFPVTEMAVGLANGVIDGTCLP
                     YEVVPAFKLQELTKVHCEPQAGARGLYANSFVVPMNKARYEAMPDDLRAVIDANSGIE
                     LSRFMGRTFDVNEQAGYELCKAQGNSFVEIPRAEMERWREATRPVVEDWIRMLDDKGH
                     PGAELVAALDGLITAASGG"
     gene            complement(2182..2346)
                     /locus_tag="VGB88_09990"
     CDS             complement(2182..2346)
                     /locus_tag="VGB88_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647819.1"
                     /translation="MRTNRRTFLILHKGDELAVEVTETRVWTEPHAEDPGRLKAVPVP
                     DYVKSRLAGP"
     gene            complement(2374..4227)
                     /locus_tag="VGB88_09995"
     CDS             complement(2374..4227)
                     /locus_tag="VGB88_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEX9647820.1"
                     /translation="MSARIRAVRLGPSGAVVERRDDGTMYLRSPHPLGPYPDRLTERL
                     VHWAAAAPDRTFLAERAEDGDWRRISYGETLAAVRAIGQALIARGLSPDRPLAILSGN
                     DIEHALLGLAALHVGVPYAPISPAYSLVSEDHAGLCAILDLLTPGLVFAADGARFAAA
                     LANARPAEAELAVTRNPPSVPAATPFAELAATRATAAVEAAFAAVGPDTVAKILYTSG
                     STGRPKGVINTQRMLCSNQAMLCAVLAFLEDEPPVIVDWLPWHHTFGGNHNVGLVLYN
                     GGTLYIDDGRPMPDGIARTVRNLREIAPTVYFNVPKGWETLAAALRRDRSLRETFFSR
                     VRLLFYAAAGLPQHVWDELESLALKSCGQSIVMLTGLGATESAPFAICCSKDTARSGA
                     IGLPVPGVELKLAPVDGKLETRLRGPSITPGYWRQPELTAAAFDDAGYFRMGDAVRFA
                     DPGDAQKGLMFDGRLAEDFKLSTGTWVSVGPLRARFIAHFAPLVHDVVIAGPDRDWPA
                     ALIFADIAACRALGRDLGSDAPLAAVLAHPKVQAEFARRLASFADAAAASSMRIGAAM
                     LLEEPASLDAHEVTDKGSINQRAVLARRARLVEELYAAPPGARVIALGVRG"
     gene            4288..5298
                     /locus_tag="VGB88_10000"
     CDS             4288..5298
                     /locus_tag="VGB88_10000"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647821.1"
                     /translation="MPAGRRTAGIAARRACDPRWARAAAQPGTSHREGGVAGSQLGFV
                     GIGRMGSLMTRRLLDAGHRVAVFDTDPRAMAALAERGAERAGSPAEVASRAETVFVSL
                     PTPDVVRAVALDADGIRDGSTVRHMVDVSTTGPRIATLVAAGLAERDILYVDAPVSGG
                     LAGAANGTLAIMLSCPRPVYEALKDTLATFGKVFHVGEGPGQGQTLKLANNLLAAAAL
                     AVTSEAIVMGVKAGLDPALMCEVINASSGRNSATQDKFPRAVLPRSFDFGFTTGLSYK
                     DVKLCIDEAEALGVPMVVGSAVRQFLAVTNALYGPDSDFTSMVRVVERWAGVEVRGTR
                     DA"
     gene            5330..6736
                     /locus_tag="VGB88_10005"
     CDS             5330..6736
                     /locus_tag="VGB88_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9647822.1"
                     /translation="MTARHRPEGAPPLARTLARAALGPAPEALGAEVAEKVGLCLLDH
                     LACAFESRTLPWSRQALALAELSSGAGTVIGGPAGVGLGDAAFANGVLGHGLVREDMH
                     AGSVSHLGVVILPALLALAQHRRVAGVDFAAAAVAGYEVGAKVGRALIDPDIALSHRP
                     TGITGPLAAAAAGARALGLGEDAAVAALALAANTTAGLNEWAHTGGSEMYFHPGFAAR
                     NALSAVLLAEAGAFASPSALDGDAGLFASLGKQSAAAAVALFTGAPEILAVYFKPVPA
                     CNFAQTACQAALRVIADGARSDDIADIAVRVPGAAAAYPGCDFTGPFDQPLQAKMSIQ
                     YNVAAALRHGGVSERNYMERDDAEVARLLAATRLEVDEALSRAFPARQGAEVVVTLRA
                     GATRRARLDDVVPATPDQVRARFRAAAEAALGEARARALEAAVDGIDRLDDAGRIAAL
                     AAPGAAAARPKHARSLGA"
     gene            6790..7749
                     /locus_tag="VGB88_10010"
     CDS             6790..7749
                     /locus_tag="VGB88_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9647823.1"
                     /translation="MDTLQFTESFLQALVAGLLIGSYYGLMCVGLSVIFGVMRVINFA
                     QGDFMMLGMYVAYYLFVGAGLGIVLGDVWGPYLVAILAGPPIFVLGYLTHRLLVGRVT
                     GIGGRALEAEGHYAQLILTLGIALILQNGGLFLFGSAPVSISTPLSYTAWEVGPLIGE
                     RVTVLVNQGKGASAVIAVCVVVAFALVMARTQLGKSLRAAADNAQAAIYVGIDVDRAH
                     RIAFAFGVAITAIAGGLLASGYTFQPYVGLEYVIVMYAGVVLGGMGSTAGAFLGGLII
                     GLVQQVSTLFLPSQLQNTAIFVIFLVIILLRPQGLFGRAAQRT"
     gene            7746..>9500
                     /locus_tag="VGB88_10015"
     CDS             7746..>9500
                     /locus_tag="VGB88_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9647824.1"
                     /translation="MSARRAGGRRGLALIGGFGLAYALATVAVDNSYYQLIMTVVPVW
                     AVMGLAWNLLSGYSGLISFGHAAFFGIGAYFVALGLIDWQLSPWLGIPIAAALGALAG
                     LLIGIPTFRLRGHYFALAMLAYPLALLYIFEWLGYQEIALPMMRAAPRAYMQFDDPRS
                     YGLIALGLLLAAMLLTHAVERSRFGMSLIAIKEDEPAAEAAGIDTLAWKLRAITLSGA
                     VAAAIGGFYAVVLLVVTPPAVFGMLVSAQALVVAMFGGVATLWGPVVGALILVPLSEV
                     LHAALGAVIPGIQGVIYGVAIVATVLLAPEGVVWRVRDLLGTRGAAPLERAAAPAPAA
                     AAGLARRPPAPDGRPVFEVRSVSKSFGGLKAIDDVSLIVRAGEILGVIGPNGAGKTTL
                     FNLLNGFIRPDAGDILLDGVSVVGQKPNRICRAGVGRTFQVVRPFARMTVADNVVVGA
                     YVRAGTDAEARAAAAEALARVGLSAEAERRAGALSSKELRLMELARALAGRPRVLLLD
                     ETLAGLGAAEIEELIAVIRRLAAGGITVVIIEHTMGAMVRLVDRFVVLDHGAVLAEGA
                     PAAITSDARVIEAYLGKKW"
BASE COUNT         1253 a         3387 c         3520 g         1340 t
ORIGIN      
        1 accatgaagc cgagcaccgc gagcagcccc ggcacggtcg cggcgaggaa cagcttgacg
       61 atgttctgct cggtgagcac ggcgtagatc accaggatga tcgacggcgg gatcaggatg
      121 ccgagggtgc cgccggcggc gagggttccc gccgacagcg ccgccgaggt gccgtgccgg
      181 cgcatctcgg gcagcgccac ctggcccatg gtggtggcgg tggcgatcga ggagccgcag
      241 atcgcgccga acgcggcgca gcccgccacc gaggccatcg ccagcccgcc gcggcggtgg
      301 ccgagccagg cgttgcaggc gtcgaagatc gaccggctga cgcccgagcg ggtggcgagc
      361 tggcccatca ggatgaatag cgggatgatg gcgaggtcgt aggagacgaa cttgtcgacc
      421 atcgcggtct tcaggtagcc cgagagcggc gcccagccgg cgagcgcgac atagccgccg
      481 gcgccggtga ggcccatggc gagcgccacc ggcaggcgca ccgcaatcag gaccacgagc
      541 gccgcgatca tcgcggcacc gaacgcgacc ggggtcacga cgggcgcgcg cctctcccgg
      601 ccgcggcgag cagccgccgg gcgctctgca gcgccaccag gcctgcgatc gcgagcgcgg
      661 ccaccgccgg caaatacacg atccaggtcg gcacgccgag gatcatgctg cggtcgccgt
      721 agccgtgcgc ctcgattccg ccgaccgtca gccgccacac cagcaccgcc caggcgagcg
      781 cgaacaagag ctcgcccgcc gcctcgagcc cgcgccgcgc gcttaccggc agccagtcgg
      841 tgaacagctc gatggtgatg tgcgcgcgct cgagctggca gatcgggaag aacaggaaga
      901 ccgcggccgc cgagccgatc tcgaccacct cgaagtcgcc cgggatcgat agcagcctaa
      961 agcttcgccc gaataccgag acgaacacgg tcagcgcgat ggcgaagatg gcgaggccgc
     1021 cgagcgcggc ggccgcccgg cacagcccgt cgaccgcccg gtcgagcgcc ccctcgggcg
     1081 cgcccgccaa tcgtcagccc ccgctcgcgg cggtgatcag gccgtcgagg gcggcaacca
     1141 gctcggcgcc gggatgtccc ttgtcgtcga gcatcctgat ccagtcctcg accaccggcc
     1201 gggtcgcctc gcgccagcgc tccatctccg cgcgcgggat ctcgacgaag ctgtttcctt
     1261 gcgccttgca cagctcatag cccgcctgct cgttgacatc gaaggtgcgg cccatgaacc
     1321 gcgacagctc gatgccggaa ttggcgtcga tcaccgccct caagtcatcg ggcatggcct
     1381 cgtagcgcgc cttgttcatc ggcaccacga agctgttggc atagaggccg cgcgcgcccg
     1441 cctgcggctc gcagtggacc ttggtcagct cctggagctt gaacgcgggt accacctcgt
     1501 agggcaggca ggtgccgtcg atgacgccgt tggcgagccc gaccgccatc tcggtgaccg
     1561 ggaagaacac cggctccgcg ccgaggatgc cgagcgcccg gccgaacacc tggtttggcg
     1621 cgcggatgcg caggccggcg aggtcggcag cgccggtgat cggcttctcg cgcatgtgga
     1681 acttgccggg cgcgtggacg tggaaggcga gcacgtggac gtccgcgaac tcgccgccgc
     1741 cataggccgc catgaacttg tgcagcgcga cgctggtcgc ctcggcggtg gtgaccatga
     1801 acggcagggt cgcggtctcg gcgagcgggt agcggccggg gttgtagacc ggcagcgccc
     1861 acgagatgtc ggcgatgccg tcgcgcacct ggtcgatcag ctgcgccggc ttgccgccga
     1921 gctgcatggc cgcgaacacc tggacctcga tgcgcccacc cgactgctcg gccacccgct
     1981 tggcccacgg cgccaggatg tcgcggtggg cgggcgagac gggcggcagc atgtggtgca
     2041 gcttgagcgt cacctcggcc gcgcgcgcgc gcccgatcag cgccggcgcg cacacacccc
     2101 cggcgatcag cgcggcggcg cgccggcgcg tgatgcgcaa tgaaccctta gccatacaag
     2161 cgtctccttc ccttgggccg gtcatggccc ggcgaggcgg ctcttgacgt aatccgggac
     2221 cggcaccgcc ttgagccgcc ccgggtcctc ggcatggggc tcggtccaca cccgcgtttc
     2281 ggtcacctcg accgcgagct cgtcgccctt gtgcagtatc aggaacgtgc gccggttggt
     2341 gcgcatggcg gccgttgcgc gatgcctccg ctctcagccg cgcacgccga gcgcgatgac
     2401 gcgcgcgccc ggcggcgcgg cgtagagctc ctcgaccagg cgggcgcggc gcgcaagcac
     2461 tgcgcgctgg ttgatcgagc ccttgtcggt gacctcgtgc gcgtcgagcg acgccggctc
     2521 ctcaagcagc atcgccgcgc cgatgcgcat cgagctcgcg gccgccgcgt cggcgaacga
     2581 ggcgagccgg cgcgcgaact cggcctgcac cttggggtgc gcgagcaccg cggcgagcgg
     2641 cgcgtcggag ccgagatcgc ggccgagcgc gcggcacgcc gcgatgtcgg cgaagatcag
     2701 cgccgccggc cagtcgcggt cgggcccggc gatcaccacg tcgtggacca gcggcgcgaa
     2761 atgggcgatg aagcgcgccc gcaacggccc gacgctgacc caggtgccgg tcgacagctt
     2821 gaagtcctcg gccaggcggc cgtcgaacat cagccctttc tgcgcatcgc ccggatcggc
     2881 gaaccgcacc gcgtcgccca tgcgaaagta ccctgcgtcg tcgaatgccg ccgcggtcag
     2941 ctcgggctgg cgccagtagc cgggggtgat cgagggcccg cgcaggcgcg tctcgagctt
     3001 gccgtctacg ggcgcgagct tgagctcgac ccccggcacc ggcaggccga tcgcgcccga
     3061 gcgcgcggta tccttcgagc agcagatcgc gaacggcgcg ctctcggtgg cgccgaggcc
     3121 ggtcagcatg acgatgctct ggccgcagct cttgagcgcc agcgactcga gctcgtccca
     3181 gacatgctgg ggaaggccgg cggcggcgta gaacagcagc cgcacccggc tgaagaaggt
     3241 ctcgcgcaag ctgcggtccc ggcgcagcgc ggcggccagt gtctcccagc ccttgggcac
     3301 gttgaagtag acggtcggcg cgatctcgcg caggttgcgc acggtgcgcg cgatgccgtc
     3361 cggcatcggc cggccgtcgt cgatgtagag cgtgccgccg ttgtacagca ccaggccgac
     3421 attgtggttg ccgccgaagg tgtggtgcca cggcagccag tcgacgatca ccggcggctc
     3481 gtcctcgagg aaggcgagca cggcgcacag catcgcctgg ttgctgcaca gcatgcgctg
     3541 ggtattgatc acgcccttgg gccggccggt cgagcccgag gtgtagagga ttttggcgac
     3601 ggtatccggg ccgaccgcgg caaatgcggc ctcgaccgcc gcggtggccc gtgtcgccgc
     3661 gagctcggcg aacggcgtcg ccgccggcac cgaaggcgga ttgcgggtga ccgcgagttc
     3721 cgcctccgcg ggccgcgcgt tggcgagtgc ggccgcgaag cgcgcgccgt cggcggcgaa
     3781 taccaggccc ggggtcagca ggtcgaggat ggcgcagagc ccggcgtggt cctcggaaac
     3841 cagcgaatag gcgggcgaga tcggcgcata cggcacgccc acgtggagcg cggcgaggcc
     3901 gaggagggcg tgctcgatgt cgttgcccga caggatcgcg agcgggcggt cgggggagag
     3961 cccgcgcgcg atcagcgcct ggccgatggc gcgcaccgcc gccagggttt cgccatagct
     4021 gatccgccgc cagtccccgt cctcggcccg ctcggccagg aaggtgcgat cgggcgccgc
     4081 ggcggcccaa tggacgagcc gctcggtcag ccggtcggga tagggcccga gcgggtgcgg
     4141 cgagcggagg tacatggtgc cgtcgtcgcg gcgctcgacc accgcccccg acggccccag
     4201 ccggacggcg cggatgcgcg cggacatctt gcctcccaga ccggcgtgcg tctgccggcg
     4261 ttatagcaaa tgcgccgggc ggagcccatg cccgcgggcc gccggaccgc cgggattgcg
     4321 gcgaggcggg cttgtgaccc aagatgggcg cgcgcggcgg cgcagccggg gacatcgcat
     4381 cgggaggggg gcgtggcggg aagccagttg gggttcgtcg gcatcggccg gatgggatcg
     4441 ctgatgaccc ggcgcctgct cgacgccggc caccgggtcg cggtgttcga caccgacccg
     4501 agggcgatgg ccgcgctcgc cgagcgcggc gccgagcgcg cgggctcgcc ggccgaggtc
     4561 gcctcgcgcg ccgagacggt gttcgtgagc ttgcccaccc ccgatgttgt gcgcgcggtg
     4621 gcgctggatg ccgacggcat ccgcgacggc agcacggtcc gccacatggt cgatgtctcc
     4681 accaccgggc cgcgcatcgc caccctggtc gcggcgggcc ttgccgagcg cgacatcctg
     4741 tacgtcgatg cgccggtcag cggcggcctc gccggcgccg ccaacggcac gctcgcgatc
     4801 atgctgtcct gcccgcgccc ggtttacgag gcgctcaagg acacgctcgc caccttcggc
     4861 aaggtgttcc acgtcggcga ggggccgggg caaggccaga cgctgaagct cgccaacaac
     4921 ctgctcgccg ccgccgcgct ggcggtgacc tcggaagcga tcgtgatggg ggtcaaggcg
     4981 gggctcgatc cggcgctgat gtgcgaggtg atcaacgcct cgagcgggcg caacagcgcc
     5041 acccaggaca agttcccgcg cgcggtgctg ccgcgcagct tcgacttcgg cttcaccacg
     5101 gggctgtcgt acaaggacgt caagctgtgc atcgacgagg ccgaggcgct cggcgtgccg
     5161 atggtggtcg gcagcgcggt gcgccagttc ctcgccgtga ccaacgcgct ctacggcccg
     5221 gactccgact tcacctcgat ggtgcgggtg gtcgagcgct gggccggggt cgaggtgcgc
     5281 ggcacgcgcg acgcctgacg ccggcctttc cgcacgcccg gaggatgcga tgaccgctcg
     5341 acaccgacca gaaggcgcgc cgccgctcgc gcgcacgctc gcccgcgccg ccctcggccc
     5401 ggcgcccgag gcgctcggcg cggaggtggc ggagaaggtc gggctgtgcc tgctcgatca
     5461 cctcgcctgc gcgttcgagt cgcgcacgct gccgtggagc cgccaggcgc tcgcgctcgc
     5521 cgagctgagc agtggcgcgg gcacggtcat cggcgggccg gcgggggtgg gcctcggcga
     5581 tgccgcgttc gccaacggcg tgctcggcca cggcctggtg cgcgaggaca tgcacgccgg
     5641 cagcgtcagc catctgggcg tggtgatcct gccggcgctg ctcgcgctcg cccagcaccg
     5701 gcgcgtcgcc ggcgtggact tcgccgccgc cgcggtggcc ggctacgagg tcggcgcgaa
     5761 ggtcggccgc gcgctgatcg atccggacat cgccctgagc caccgcccga ccggcatcac
     5821 cgggccgctc gccgcggcgg cggcgggggc gcgcgcgctc ggtctgggcg aggacgcggc
     5881 ggttgccgcg ctcgccttgg ccgcgaacac caccgccggg ctcaacgagt gggcgcacac
     5941 cggcggctcg gagatgtact tccatcccgg cttcgcggcg cgcaacgcgc tgagcgcggt
     6001 gctgctcgca gaagccggcg cgttcgcctc gccgagcgcg ctcgacggcg acgccgggct
     6061 gttcgcctcg ctcggcaagc agtcggcggc ggcggcggtc gcgctgttca ccggcgcgcc
     6121 cgagattctc gccgtctact tcaagccggt accggcgtgc aacttcgccc agaccgcgtg
     6181 ccaggcggcg ctgcgcgtga tcgcggacgg cgcgcgcagc gacgacatcg cggacatcgc
     6241 ggtgcgcgtg ccgggcgccg ccgcggctta tccgggctgc gacttcaccg gcccgttcga
     6301 ccagccgctc caggccaaga tgagcatcca gtacaacgtc gccgcggcgc tccgccacgg
     6361 cggggtttcg gagcgcaact acatggagcg cgacgacgcc gaggtcgcgc ggctgctggc
     6421 ggcgacgcgg ctcgaggtcg acgaggcgtt gagccgcgcc tttcccgcca ggcagggcgc
     6481 cgaggtggtg gtgaccttgc gcgcgggcgc aacccggcgc gcgcgcctcg acgacgtggt
     6541 gccggcgacc cccgatcagg tccgcgcccg cttccgcgcc gccgccgagg cggcgctcgg
     6601 ggaggctcgt gcgcgcgcgc tcgaggcggc ggtggacggg atcgaccggc tcgacgatgc
     6661 cggccggatc gcggcgctcg ccgcgcccgg cgcggccgcg gcccggccca agcacgcgcg
     6721 atcgctcggc gcctgagccg gccgagccga ccgcgtccgt cgaggcagcc ggcaatcggg
     6781 accggacggg tggatacgct gcagttcacc gagagcttcc tgcaggcgct ggtcgccggg
     6841 ctgctgatcg gctcgtacta cgggctgatg tgcgtcggct tgagcgtcat cttcggggtc
     6901 atgcgggtga tcaacttcgc ccaaggcgac ttcatgatgc tgggcatgta tgtcgcctac
     6961 tatctgttcg tcggcgcagg gctcggcatc gtgctcggcg atgtttgggg cccctatctg
     7021 gtcgcgatcc tggccgggcc gccgatcttc gtgctcggct acctgacgca ccggctgctg
     7081 gtcggcaggg tgaccggcat cggcggccgc gcgctcgagg ccgagggcca ctatgcgcag
     7141 ctgatcctga ccttgggcat cgcgctgatc ctgcagaacg gcgggctgtt cctgttcggc
     7201 tcggcgccgg tgtcgatctc gaccccgctg tcctatacgg cctgggaggt cggcccgctg
     7261 atcggcgagc gggtgaccgt gctcgtcaac cagggcaagg gcgcctcggc ggtgatcgca
     7321 gtgtgcgtgg tggtcgcctt cgcgctggta atggcgcgca cgcagctcgg caagtcgctg
     7381 agggcggcgg ccgacaatgc gcaggcggcg atctatgtcg gcatcgacgt cgaccgcgcc
     7441 caccgcatcg ccttcgcctt cggcgtcgcg atcaccgcca tcgccggcgg cctgctggcg
     7501 tcgggctaca ccttccagcc ctatgtcggg ctcgaatacg tgatcgtgat gtatgccggg
     7561 gtggtgctgg gcggcatggg cagcaccgcg ggcgcgttcc tgggcgggct gatcatcggc
     7621 ctggtgcagc aggtctcgac cctgttcctg ccgagccagc tccagaacac cgccatcttc
     7681 gtcatcttcc tggtcatcat cctgctccgg ccgcagggcc tgttcgggcg cgcggcgcaa
     7741 cggacatgag cgcgcgccgg gcgggcgggc gccgcggtct cgccctgatc ggcggcttcg
     7801 ggctggctta cgcgctcgcc accgtcgcgg tcgacaactc ctactaccag ctgatcatga
     7861 ccgtggtgcc ggtgtgggcg gtgatgggcc tcgcctggaa cctgttgagc ggctacagcg
     7921 gcctgatctc gttcggccac gcggcgttct tcggcatcgg cgcctacttc gtcgccctcg
     7981 ggctgatcga ctggcagctc agcccgtggc tcggcatccc gatcgccgcg gcgctcggcg
     8041 cgctcgccgg gctcttgatc ggcatcccga ccttccgcct gcgcgggcac tacttcgcgc
     8101 tcgccatgct cgcctacccg ctggcgctgc tctacatctt cgaatggctc ggctaccagg
     8161 agatcgcgct gccgatgatg cgcgccgcgc cgcgcgccta catgcagttc gacgatccgc
     8221 gcagctacgg gctgatcgcg ctcggcctgc tgctcgccgc catgctgctc acccacgcgg
     8281 tcgagcgctc gcgcttcggc atgtcgctga tcgcgatcaa ggaggacgag ccggcggccg
     8341 aggccgcggg catcgacacc ctcgcctgga agctcagggc gatcaccttg agcggcgccg
     8401 tcgccgccgc catcggcggc ttctacgcgg tggtcctgct ggtggtcacg ccgcccgcgg
     8461 tgttcggcat gctggtctcg gcccaggcgc tggtggtggc gatgttcggc ggcgtcgcca
     8521 cgctctgggg gccggtggtc ggggcgctga tcctagtgcc gttgagcgag gtcttgcacg
     8581 ccgcgctcgg cgccgtgatc ccgggcatcc agggggtgat ctacggggtg gcgatcgtgg
     8641 ccacggtcct gcttgcgccc gagggcgtgg tctggcgggt gcgcgacctg ctcggcacgc
     8701 gcggcgcggc gccgctggag cgggccgcgg cgccggcgcc cgctgccgcg gccgggcttg
     8761 cgcgccggcc gccggcaccg gacgggcggc cggtgttcga ggtgcgcagc gtgtcgaagt
     8821 cgttcggcgg gctcaaggcg atcgatgacg tgagcctgat cgtgcgcgcg ggcgagatcc
     8881 tgggcgtgat cgggcccaac ggcgccggca agacgacgct gttcaacctg ctcaacggct
     8941 tcatccgccc cgacgccggc gacatcctgc tcgacggcgt ctcggtggtc gggcagaagc
     9001 ccaaccgcat ctgccgcgcc ggcgtcgggc gcaccttcca ggtggtgcgc ccgttcgcgc
     9061 gcatgaccgt ggccgacaac gtcgtggtcg gcgcctacgt ccgcgccggg accgacgccg
     9121 aggcccgcgc ggccgccgcc gaggcgctcg cccgcgtcgg cctcagcgcc gaggccgaga
     9181 ggcgcgccgg cgcgctgtcg agcaaggagc tgcgcctgat ggagcttgcc cgtgcgctcg
     9241 ccggccgacc ccgggtgctg ctcctcgacg agaccctcgc cgggctcggc gcggccgaga
     9301 tcgaggagct gatcgcggtg atccggcgcc tggccgcggg cggcatcacg gtggtgatca
     9361 tcgagcacac catgggcgcc atggtgcgcc tggtcgaccg cttcgtggtg ctcgaccacg
     9421 gcgcggtgct cgccgagggc gcgcccgccg caatcaccag cgacgcgcgc gtgatcgagg
     9481 cctatctcgg caagaagtgg
//
LOCUS       DASWHU010000270         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3457724, whole genome shotgun sequence.
ACCESSION   DASWHU010000270 DASWHU010000000
VERSION     DASWHU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457724"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /gene="tatC"
                     /locus_tag="VGB88_10020"
     CDS             complement(<1..555)
                     /gene="tatC"
                     /locus_tag="VGB88_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9647825.1"
                     /translation="MADTPENEIEASKAPLLDHLIELRNRLMWSVAALFVAFIGCYFV
                     SSDIYAFLVRPLAEIYADMGVEHPRMIYTALHEAFFTYMKVAFYAALFISLPVIANQF
                     WKFVAPGLYRHERKAFLPFMFATPLLFALGAGLVYYFIFPLAWKFFLSFQTAGTDNGL
                     AIELQAKVNEYLSLVVRLMFAFGIA"
     gene            complement(665..1162)
                     /gene="tatB"
                     /locus_tag="VGB88_10025"
     CDS             complement(665..1162)
                     /gene="tatB"
                     /locus_tag="VGB88_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX9647826.1"
                     /translation="MFDIGWSEMAVIVVVALFVVGPRDLPKVIRTFGRYAGKIRGMAR
                     EFQDSIDEAVREAELDEVKKQIEQAARFDIDKTISNTVDPGGEIGKAMDMTATAKPAA
                     VAPPAQPQGAKGMAAAAAGAAKAANGSGGAPAAGAAKAAEAAPPAPSAPGAEEGGREP
                     DKAGA"
     gene            complement(1228..1491)
                     /gene="tatA"
                     /locus_tag="VGB88_10030"
     CDS             complement(1228..1491)
                     /gene="tatA"
                     /locus_tag="VGB88_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9647827.1"
                     /translation="MGPSFWQIVLILVIVLILFGAGKLPRVMGDVAKGVKSFKAGMKE
                     DVGEDAPAPKEVQHTEPAAAATVQRAAEPVKETVVKSDEPAKT"
     gene            complement(1705..2352)
                     /gene="scpB"
                     /locus_tag="VGB88_10035"
     CDS             complement(1705..2352)
                     /gene="scpB"
                     /locus_tag="VGB88_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9647828.1"
                     /translation="MSAPPPAPEREAMRIVEALLFASRAPLGEAELAAHLPEDTALAP
                     LLARLVDSYAERGVNLVRVAEGWALRTAPDLAPALRREAEVQRKLSRAALETLAIVAY
                     HQPVTRAEIEQIRGVAVSKGTLDILFEQGWIQPRGHRRSPGRPATWVTTPSFLDHFGL
                     ASLDDLPGLDELRAAGLLDARAAAPGLALALAGGEADEDGEAPEEPEAEQLDDLD"
     gene            complement(2349..3155)
                     /locus_tag="VGB88_10040"
     CDS             complement(2349..3155)
                     /locus_tag="VGB88_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX9647829.1"
                     /translation="MSEGEMGLEFRDDFDDAADAAGPAQLVLDLDGFEGPIDVLLTLA
                     REQKVDLLHISILQLADQYLDYVRRARRQRLELAADYLVMAAWLAYLKSRLLLPEDGA
                     EGEPSGEEMAEALAFQLRRLEAMREAAAKLMARDLLGRDVFARGVPEDISPLAVPVYA
                     LTLNDLLSAYADQRRRRVAVASFHLGPIRLMTLEAAIRRLSGMLGMALEWTTLARFLP
                     PDLGDPLLARSALASTFAATLELCRSGRIRLRQERPFAPLFVRATPEGGR"
     gene            complement(3152..3832)
                     /locus_tag="VGB88_10045"
     CDS             complement(3152..3832)
                     /locus_tag="VGB88_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9647830.1"
                     /translation="MNDILYTLSVWALPVIIAVTFHEAAHGWVAWKLGDETAYRLGRV
                     TFNPLKHIDPFGTILLPALLFLSRMPFVLGYAKPVPVNFRALRHPRRDSVLVALAGPG
                     TNIALALASAVLVHGAALVPAAAGPWLTENLTNSILVNLVLAVFNMLPIPPLDGGRVA
                     VGLLPRALARPLARLERHGLLILIGLLFLLPWLSRSLGYDVNILAWVIWRPVVALFGL
                     VTRLTGLA"
     gene            complement(3829..4878)
                     /gene="nagZ"
                     /locus_tag="VGB88_10050"
     CDS             complement(3829..4878)
                     /gene="nagZ"
                     /locus_tag="VGB88_10050"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX9647831.1"
                     /translation="MSRPDRDKKPKPARAVIFGCAGLTLAGAERRLFAEADPLGFILF
                     KRNLRKPEQARALVASLRAAVGRADAPVLIDQEGGRVARLGRPYWRVAPAAARFGALA
                     RIDRFVAAEAAALNSRLIAHELHDLGIDVDCLPVLDVPAAGANAVIGDRAYAADVELV
                     AMLGRAACGGLLAGGVLPVIKHIPGHGRARADSHHALPVVETPLAELDRTDFAPFRAL
                     ADMPMAMTAHVVYAAVDAERPATISAAVIGEVIRARIGFSGLLLSDDIGMAALSGALA
                     LRARAALAAGCDVVLHCSGDLEEMRQVAGACASMSEAAQARWARAAALRRTPQPFDSA
                     RARAHVDELLKGVPA"
     gene            complement(4865..>5414)
                     /locus_tag="VGB88_10055"
     CDS             complement(4865..>5414)
                     /locus_tag="VGB88_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9647832.1"
                     /translation="ADPSPVRERPADPGGMQVPYQDSLILENLGEETPAPTVERLLPP
                     PEQPLEPPVEQPVATEASAAPALPAPSPPPAPPGPEETAAALAPAPAVLVPPDPFAGF
                     RVQVGSFRSREEAEAGWIEALANGGELLAGVGHAVAEVDLGADRGIFYRLQAGPLPSW
                     DGAEGLCAELKVRQMDCFVVAP"
BASE COUNT          825 a         1985 c         1916 g          688 t
ORIGIN      
        1 cgcgatgccg aaggcgaaca tcaggcgcac caccagcgac aaatactcgt tgaccttggc
       61 ctggagctcg atcgccagcc cgttgtcggt gccggcggtc tggaagctga ggaagaactt
      121 ccaggcgagc gggaagatga agtagtagac cagccccgcg ccgagcgcga acaagagcgg
      181 ggtcgcgaac atgaacggca ggaacgcctt gcgctcgtgg cggtacaggc ccggtgccac
      241 gaacttccag aactggttgg cgatcaccgg cagcgagatg aacagcgcgg cgtagaacgc
      301 caccttcata taggtgaaga aggcctcgtg caacgcggtg tagatcatgc gcgggtgctc
      361 gacgcccatg tcggcgtaga tctcggccag cgggcgcacc aggaaggcat agatgtccga
      421 ggacacgaag tagcagccga tgaaggcgac gaacagcgcc gccaccgacc acatcagccg
      481 gttgcgcagc tcgatcaggt gatcgagaag cggtgccttc gaggcctcga tctcgttttc
      541 aggcgtatcg gccacgtgct gacccgaagc atccggatgc cgacccgtcc gcccgcgccg
      601 ggcgcgcggc acgaccactc cgagctggat accgaacgtg cgtcaccggc gggatcccct
      661 gatatcaggc gccggccttg tcgggctccc ggcccccttc ctcggcgccg ggcgcgctcg
      721 gcgccggcgg agcggcctcg gccgccttgg ccgcgccggc ggctggcgcc ccgccgctgc
      781 cgttggcggc cttcgccgca ccggcggcgg ccgcggccat ccccttggcg ccttgcggct
      841 gcgccggcgg cgccaccgcg gccggcttgg cggtcgcggt catgtccatg gccttgccga
      901 tctcgccgcc cgggtcgacg gtattggaga tcgtcttgtc gatgtcgaac cgcgccgcct
      961 gctcgatctg cttcttgacc tcgtcgagct cggcctcgcg caccgcctcg tcgatgctgt
     1021 cctggaactc gcgcgccatg cccctgatct tgccggcgta gcggccgaag gtacggatca
     1081 ccttgggcag gtcccgcggg ccgacgacga acagggcgac caccacgatg accgccatct
     1141 cgctccagcc gatatcgaac atcgcggcac gtccgtttcg ctgccgtcag gcgcggcggc
     1201 gcagcctggg ccacgcccgg cggcggttca ggtcttggcc ggctcgtccg acttcaccac
     1261 cgtctccttg accggctcgg cggcgcgctg gaccgtcgcg gcggcggccg gctcagtgtg
     1321 ctgcacctcc ttcggtgccg gcgcgtcctc gccgacgtcc tccttcattc cggccttgaa
     1381 gctcttcacc cctttcgcga cgtcgcccat cacccggggc agcttgccgg cgccgaacag
     1441 gatcaggacg atgaccagaa tcagtacgat ctgccaaaaa ctggggccca tctgttcact
     1501 cctcgcggat ttgcgcgcga ccgattgccc gcctcggacc gcattatcgt tgtgccgggc
     1561 ggggcaaaag cgcgcgcccg gcgcaggccc cggcccggcg aaccaagcgt agtacttagc
     1621 gatcgccggg gggaaataca aatatcatcg ccggttcggg cgagactctc aacctttcgc
     1681 ctacgcgcgg tagcgccggc cggatcagtc gagatcgtcg agctgctcgg cctcgggctc
     1741 ctccggggcc tcgccgtcct cgtccgcctc gccgccggcg agcgccagcg cgaggcccgg
     1801 ggcggcggcg cgggcgtcga gcaatccggc cgcgcgcagc tcgtccaggc cgggcaggtc
     1861 gtcgagcgag gccaggccga aatggtcgag gaagctcggc gtggtcaccc aggtcgccgg
     1921 ccggcccggg ctacggcggt ggccgcgcgg ctggatccag ccctgctcga acaggatgtc
     1981 gagggtgccc ttgctcaccg ccacgccgcg gatctgctcg atctcggcgc gggtgaccgg
     2041 ctggtggtag gcgacgatcg ccagcgtctc cagcgccgcg cgcgacagct tgcgctggac
     2101 ctcggcctcg cgcctgagcg cgggcgcaag gtcgggcgcg gtgcgcagcg cccagccctc
     2161 ggcaacgcgc accaggttga cgccgcgctc ggcataggag tcgacaaggc gcgcgagcag
     2221 cggcgcaagc gccgtgtcct cgggcagatg ggcggcaagc tcggcctcgc cgagcggcgc
     2281 gcgcgaggcg aacagcagcg cctcgacgat ccgcatcgcc tcgcgctcgg gcgccggcgg
     2341 cggcgcgctc atcggccacc ctccggtgtg gcgcgcacga acagcggcgc gaacggccgc
     2401 tcctggcgca gccggatgcg ccccgaccgg cacagctcga gggtggcggc gaaggtcgag
     2461 gcgagcgccg agcgggcgag cagcgggtcg ccgaggtcgg gcggcaggaa gcgggcgagc
     2521 gtggtccatt cgagcgccat gccgagcatg cccgacaggc gccggatcgc ggcctcaagg
     2581 gtcatcagcc ggatcgggcc gagatggaag ctcgccaccg ccacccggcg ccggcgctgg
     2641 tcggcatagg ccgacaggag gtcgttgagg gtgagcgcgt agaccggcac ggccagcggc
     2701 gagatgtcct caggcacccc gcgcgcgaac acgtcgcgcc ccaacaggtc gcgcgccatc
     2761 agcttcgccg ctgcctcgcg catcgcctcc agccggcgca gctggaaggc cagcgcctcg
     2821 gccatctcct cgccgctcgg ctcgccctcc gcgccgtcct cgggcaagag caggcgcgac
     2881 ttgagatagg cgagccacgc cgccatcacc agatagtcgg cggcgagctc gagccgctgg
     2941 cggcgggccc ggcgcacata gtcgagatac tggtcggcga gctgcaggat cgagatgtgg
     3001 agcaggtcga ccttctgctc gcgcgcgagc gtcagcagca cgtcgatcgg cccctcgaag
     3061 ccgtcgaggt cgagcaccag ctgggcgggc ccggccgcgt ccgccgcgtc gtcgaaatcg
     3121 tcgcgaaact cgagccccat ctcgccctcg ctcacgccag gccggtcagc ctggtgacca
     3181 gcccgaacag cgccaccacc ggccgccaga tgacccaggc cagaatgttg acatcatagc
     3241 ccagcgaccg gctcagccac ggcagaagaa acaaaagtcc gatcaggatc aagagaccgt
     3301 ggcgctcaag ccgcgccagc ggccgggcca gcgcccgcgg cagcagcccg accgccaccc
     3361 ggccgccgtc gagcggcgga atcggcagca tgttgaacac cgcgagcacc aggttgacca
     3421 ggatcgagtt ggtcaggttc tcggtcagcc acggccccgc cgctgccggc accagcgccg
     3481 cgccgtggac caggaccgcc gaggccagcg ccagggcgat attggtgccc gggccggcaa
     3541 gcgcgaccag caccgagtcg cggcgcgggt ggcgcagcgc gcggaagttc accggcaccg
     3601 gcttggcgta gccgagcacg aacggcatcc gcgacaggaa cagcagcgcc ggcagcagga
     3661 tggtgccgaa cgggtcgatg tgcttgagcg gattgaaagt tacccgcccc aggcggtacg
     3721 cggtttcgtc gccgagcttc cacgccaccc agccgtgcgc cgcctcgtgg aaggtgaccg
     3781 cgatgatcac cggcagcgcc cataccgaca gcgtgtagag aatatcgttc atgccggcac
     3841 gcccttgagc agctcgtcga catgggcgcg cgcgcgcgca gaatcgaacg gctgcggggt
     3901 acggcggagt gccgccgcac gcgcccagcg cgcctgcgcc gcctccgaca tcgacgcgca
     3961 cgccccggcc acctggcgca tctcctcgag gtcgccgctg caatggagca ccacgtcgca
     4021 accggcggcg agcgcggcgc gcgcgcgcaa ggccagcgcc cccgacagcg ccgccatgcc
     4081 gatgtcgtcg ctcagcagca ggccggaaaa gccgatgcgg gcgcgaatca cctcgccgat
     4141 caccgccgcc gagatcgtcg ccggccgctc ggcgtcgacc gcggcataga ccacgtgtgc
     4201 ggtcatcgcc atcggcatgt cggcgagcgc gcggaacggc gcgaaatcgg tgcggtcgag
     4261 ctcggcgagc ggcgtctcga ccaccggcag ggcgtggtgg ctgtcggcgc gcgcgcggcc
     4321 gtggccgggg atgtgcttga tcaccggcag cacgccgccg gcgagcagac cgccgcacgc
     4381 cgcccggccg agcatcgcga ccagctcgac atccgccgcg taggcgcgat cgccgatcac
     4441 cgcgtttgcg cccgccgccg gcacatcgag caccggcagg cagtcgacgt cgatgccgag
     4501 gtcgtggagc tcgtgcgcga tcaggcggct gttgagcgcc gcggcctcgg cggcgacgaa
     4561 gcggtcgatg cgcgccagag cgccgaaccg cgccgcggcc ggcgcaaccc gccagtacgg
     4621 ccgccccagc cgcgcgacgc gcccgccctc ctggtcgatc agcaccggcg catccgcacg
     4681 cccgaccgcg gcgcgcaacg aggcgaccag cgcgcgcgcc tgctcgggct tgcgcaaatt
     4741 gcgcttgaac aggatgaagc cgagcgggtc ggcttcggcg aacaggcgcc gctcggcgcc
     4801 ggcgagcgtc aagccggcgc agccgaagat caccgcgcgc gcgggcttgg gtttcttgtc
     4861 ccggtcaggg cgcgacaacg aagcagtcca tctgccggac cttgagctcg gcgcacaggc
     4921 cttcggcgcc gtcccagctc ggcagcggcc ccgcctgcag ccgatagaag atgccccgat
     4981 cggcgcccag atcgacctcg gcgaccgcat ggccgacgcc ggcgagcagc tcgccgccat
     5041 tggcgagcgc ctcgatccag cccgcctccg cctcctcgcg gctgcggaac gaaccgacct
     5101 ggacccggaa gccggcaaac gggtcgggcg gtaccagtac cgccggtgcc ggcgccaacg
     5161 ccgcggcggt ctcctccggc cccggcggcg ccggcggcgg cgacggcgcg ggcagcgccg
     5221 gcgcggccga cgcctcggtc gcgaccggct gctcgaccgg cggttcgagc ggttgctccg
     5281 gcggcggcag cagccgctcg acggtcgggg cgggcgtctc ctcgccgagg ttctccagga
     5341 tcagcgagtc ctggtagggc acctgcatgc cgccgggatc ggccgggcgc tcgcgcaccg
     5401 gcgaggggtc ggcg
//
LOCUS       DASWHU010000271         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2385386, whole genome shotgun sequence.
ACCESSION   DASWHU010000271 DASWHU010000000
VERSION     DASWHU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385386"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGB88_10060"
     CDS             <1..228
                     /locus_tag="VGB88_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647833.1"
                     /translation="APVLESYARARSFDTVSMSLVTDGLNRLFSNDSAPLRLARDLGL
                     AAVDRSAPLKRLFMRHAMGLIGTQSSERLAD"
     gene            complement(245..>1510)
                     /locus_tag="VGB88_10065"
     CDS             complement(245..>1510)
                     /locus_tag="VGB88_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9647834.1"
                     /translation="ATVALALLAWDLAGAEAGAVLGTALAIKMVAYVGVAPVAGSLAD
                     RLPRRATLVALDIVRAGVALALPFVDQVWQVYLLIFLLQAASAAFTPLFQATIPEVLP
                     DERDYTKALSLSRIAYDLESLLSPLLAAALLAVTSFHVLFAGTALGFAASAALVGATV
                     LPRLEPVRARRFADRLTRGVRVFLRTPRLRGLLAVNLAVAAGGAMAIVNTVVYVRDRL
                     GLGEGETALALAAFGAGSMLVAVALPPLLARLSDRPVMIAGAAAIAAVLLAAPLLDAQ
                     LAGLFALWVVLGAGYSLAQTPTGRVLRRSSSAADRPALFAAQFALSHAGWLLAYPLAG
                     WAGAALGLDAAFLALGAIATAAASAAALLWPRTDAESLVHVHTDLVPGDPHLAGAEPT
                     AAGYKHRHALVIDDAHGHWPWPDGAMHRL"
BASE COUNT          243 a          548 c          573 g          146 t
ORIGIN      
        1 gccccggtgc tcgagtctta tgcgcgcgcg cgcagcttcg acacggtcag catgagcctg
       61 gtgaccgacg ggctcaaccg cctgttcagc aacgacagcg cgccactccg cctggcccgc
      121 gacctcggcc tcgccgcggt cgaccgctca gccccgctca agcgcctgtt catgcgccac
      181 gccatgggcc tgatcggcac tcagtcgagc gaacggttgg ccgactgagt acccgatagc
      241 ggccctacag ccggtgcatg gcgccgtccg gccacggcca atggccgtgg gcgtcatcga
      301 tcaccagggc gtggcggtgc ttatagcctg ccgcggtcgg ctccgccccg gcaaggtgcg
      361 gatcgccggg cacgagatcg gtatggacat ggaccaaact ctcggcgtcg gtgcgcggcc
      421 acagcagcgc ggccgccgat gcggcggcgg tcgcgatcgc gccgagtgcc aggaaggcgg
      481 catcgaggcc gagcgcggcg cccgcccagc cggccaacgg gtaggcgagc aaccagccgg
      541 cgtggctgag cgcgaactgg gcggcgaaca gggcggggcg gtctgcggcc gacgacgagc
      601 ggcgcagcac ccgcccggtc ggggtctggg ccagcgaata gccggcgccg agcacgaccc
      661 agagcgcgaa cagcccggcg agctgcgcat cgagcagcgg cgcagcgagc agcaccgcgg
      721 cgatcgcggc ggcgcccgcg atcatcaccg gccggtccga caggcgggcg agcagcggcg
      781 gcagggcgac ggcgaccagc atcgagccgg cgccgaacgc ggccagggcg agcgcggtct
      841 cgccctcgcc gaggccgagc cggtcgcgga catagaccac cgtgttgacg atcgccatcg
      901 ccccgcccgc ggcgaccgcc aggttgaccg cgagcaggcc acgcagccgg ggcgtgcgca
      961 ggaacacgcg caccccgcgc gtgagccggt cggcgaagcg ccgcgcgcgc accggctcga
     1021 ggcgcggcag gaccgtcgcg ccgaccagcg ccgccgaggc ggcgaaaccg agcgcggtgc
     1081 cggcgaacag gacatggaag ctggtcaccg ccagcagcgc cgcggccagc agcgggctca
     1141 gcaggctctc gagatcgtag gcgatgcgcg acagcgacag cgccttggtg tagtcgcgct
     1201 cgtcgggcag cacctcgggg atggtcgcct ggaacagcgg cgtgaacgcc gccgaggccg
     1261 cctgcagcag gaagatcagg agatagacct gccagacctg gtcgacgaac ggcagcgcga
     1321 gcgcaacgcc tgcgcgcacg atgtcgagcg cgaccagggt ggcgcgccgc ggcagccggt
     1381 cggcgaggct gccggcgacg ggcgcgacgc cgacataggc gaccatcttg atcgcgagcg
     1441 cggtgccgag caccgccccg gcctcggccc cggcgaggtc ccaggcgagc aacgcgagcg
     1501 cgaccgtggc
//
LOCUS       DASWHU010000272         7583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1253228, whole genome shotgun sequence.
ACCESSION   DASWHU010000272 DASWHU010000000
VERSION     DASWHU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253228"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..405)
                     /locus_tag="VGB88_10070"
     CDS             complement(79..405)
                     /locus_tag="VGB88_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX9647835.1"
                     /translation="MSEKVVPFRRKEPAPGPTRPELVSKMRELIKDSTNIRYNHDHIK
                     MRFNQRNIIMRHVLDTLRYGRVISGPTLDKYGDWRIKLTRRVAGRRVQVVVAIKEDHV
                     VIVTAI"
     gene            complement(562..1191)
                     /locus_tag="VGB88_10075"
     CDS             complement(562..1191)
                     /locus_tag="VGB88_10075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9647836.1"
                     /translation="MAKEVRTAWWWVRHARVIANRGRLYGASDPPAELDDGDRLAAFA
                     AALPQDAVWLTSALRRARDTASAIRARGLAGAAVAVEPAFAEQSFGRWQGLTYEQVAR
                     LADKPGHRFWYTTADHAPPEGESFVAVMARVAAAIDRHTRAHAGRDVVVVAHGGPIRA
                     AIAHALGLDADAALAFVTDNLSLTRLDHVASEAPGPGWQVQFINRMVLS"
     gene            complement(1196..2008)
                     /locus_tag="VGB88_10080"
     CDS             complement(1196..2008)
                     /locus_tag="VGB88_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647837.1"
                     /translation="MSAHPAAQSWRRIFALMLRHFYLMRGSWPRVLDLIYWPTMQMVL
                     WGFITLFLITNSTWVAQAAGVLLSAVLLWDILFRGNISLFLSFEEEVWSRNLGHLMVS
                     PLRPGEFVAALIATSLVRTVIGVGGAAALAAVMFDYSILDLGLPLAAFFVNLIVLGWA
                     LGLLVSGMVLRFGQGAESLAWAAVFLIQPVSGVYYPVAVLPDWLQPVARALPSAHVFE
                     GMRALLVEHRLRLDLLAQASLLNLVYLGIGVAAFLGTMRMARVKGLLLQIGE"
     gene            complement(2005..2766)
                     /locus_tag="VGB88_10085"
     CDS             complement(2005..2766)
                     /locus_tag="VGB88_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647838.1"
                     /translation="MAEPVVQVDGLTKRFGRVVAVDGVSFAIAGGRTVGLLGGNGAGK
                     TTTIGILLGLIVPDAGAVRVLGEDVLRHRFRVLPRMNFSSPYVEVPHRLSVRENLTVY
                     GHLYGLADIAARIAELARDLDLGPLLKRKVGTLSAGQKTRVALAKALLNEPELLLLDE
                     PTVSLDPDIADYVRGYLQRYQRASGATILLASHNMAEVERLCADVIMLKAGRVIDRGA
                     PDALLDRYGRATLEEVFLDIARDRPHPVAAAGGAA"
     gene            complement(2856..3575)
                     /locus_tag="VGB88_10090"
     CDS             complement(2856..3575)
                     /locus_tag="VGB88_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX9647839.1"
                     /translation="MTEAAEGRRPLWRRLVPLALLAAAIALAFALGLDDYLSFEALRA
                     NRERLLELVQRYGVVAGIGYMAVYAGVVALSLPGATFMTLAGGFMFGAALATLYTVIG
                     ATAGATVIFLIARTAAGDVLARRAGPALRRMEQGFRDNALNYLLVLRLIPLFPFWLVN
                     LVPALLEVPLRTYVIGTFFGIIPGTFVYCLAGAGLGSVLESGAEFSLAGVLTPTMIAA
                     LAGLALLALLPIAYKRLRGGR"
     gene            complement(3600..4454)
                     /locus_tag="VGB88_10095"
     CDS             complement(3600..4454)
                     /locus_tag="VGB88_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX9647840.1"
                     /translation="MAEPPGGARGAWLRVVAGAMLCVAAGVGPAAAAPRAELWDRWLA
                     NDPASTIEVDHGAWGRLIARFTRPGADGVTRFDYAAVAPEDKALLKTYLDALAASPVS
                     RLGRAEQLAYWINLYNALTVQVVLDHYPVDSIRDIDISPGLFADGPWGRELITVEGEG
                     VSLDDIEHRILRPIWRDARLHYALNCASIGCPELYKEAFTANTAEACLEDSARAFINH
                     PRGVTVTGRGLRLSRIYDWYRDDFGASDAELFAHLRRYGEPPLAAALAASPRILGYDY
                     DWRLNDVE"
     gene            complement(4479..5345)
                     /locus_tag="VGB88_10100"
     CDS             complement(4479..5345)
                     /locus_tag="VGB88_10100"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX9647841.1"
                     /translation="MATQRCIRLVTLAMMAPALAACPSTEQAETDPGSNADSRAVWFV
                     NDPGSTETIDHSPWEEFLQKYHRELPDGVNRIDYDAVSDDDHIALKQYLSYLGTIQIE
                     TYSRDEQIAFWMDLYNAAIVDLVLDSMPVNSVLQIRGPGINVVGPWLRPVAQVHGQFV
                     SFNDIEHYILRAAFNDMGPPIHYGVNCASMGCPSLAPHAHTAQNWRQNLERIAHQFIN
                     GPEGVRFEDGQMYTSKVYHSWFKEDFGGNDKSVIEHLLKYAEPELAAELKKHSAIAGD
                     YYDWRLNKPDGS"
     gene            5582..5716
                     /gene="rpmH"
                     /locus_tag="VGB88_10105"
     CDS             5582..5716
                     /gene="rpmH"
                     /locus_tag="VGB88_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9647842.1"
                     /translation="MKRTYQPSVLVRKRRHGFRTRMASPGGRRVIAMRRAKGRKRLSA
                     "
     gene            5740..6117
                     /gene="rnpA"
                     /locus_tag="VGB88_10110"
     CDS             5740..6117
                     /gene="rnpA"
                     /locus_tag="VGB88_10110"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9647843.1"
                     /translation="MRRLKRRQDFLRVARIGRKWAAPGLVLQCAPALAGGAGVPAVGF
                     TASRRVGGAVARNRARRRLRAAADQVIATSARPDLDYVLIARTATLSRRFDRLAADLM
                     TALAKLGALAEQAAGEAPAERRQ"
     gene            6114..6371
                     /gene="yidD"
                     /locus_tag="VGB88_10115"
     CDS             6114..6371
                     /gene="yidD"
                     /locus_tag="VGB88_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9647844.1"
                     /translation="MTLAARALAVLVHGYRLLVRPLLPASCRFTPSCSDYALEALARH
                     GAGRGALLAALRLCRCHPWGGFGYDPVPDDARAWRRRGERA"
     gene            6384..>7583
                     /gene="yidC"
                     /locus_tag="VGB88_10120"
     CDS             6384..>7583
                     /gene="yidC"
                     /locus_tag="VGB88_10120"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9647845.1"
                     /translation="MQDQRNLLLAIVISVAILYGSQLLFPSPPPEPPAPAETTGAPLP
                     APAEVPAAGVPTPPAPAGAEAPAPAATPAGPRVTIERGAHGRLTGSIALIGGRLDSLT
                     LADYQETVDADSPPIRLLAPPGSAHPYFADFGWTADADAGIALPGPDTPWRASGATLT
                     PETPVTLSWDNGAGLTFERTVAVDDDFMFTVTQRVRNHGEAAVTLYGYGLVSRTDEPK
                     TLGYYILHEGPLGVLDGKLKEVDYDDLREALTIDQSTTGGWIGITDKYWLAALIPDQA
                     STVRTRFSFIRSDGRDKFQVDYLLPALEVAPGASAEVSSRLFAGAKEVTLLDRYEEQL
                     GIARFDLAVDFGWFYFLTKPLFYVIHTFSRWLGNFGLAILLLTVLIKLVFYPLANKSY
                     TAMSKLKK"
BASE COUNT         1183 a         2657 c         2616 g         1127 t
ORIGIN      
        1 agatgttatc cagtccacac tccgtgtact ggtacgcctc cctcgtgatg atgttcatag
       61 ccgcctcctg tattattttc aaattgcagt gacaataact acatgatctt ccttaatcgc
      121 tactacaact tgcactcggc ggcccgcaac acgtcttgtt agttttatcc gccaatctcc
      181 gtatttatca agcgtaggtc cactgattac cctaccatat cttaatgtat cgagtacatg
      241 tcgcataatg atatttcgtt gattgaacct cattttgatg tgatcgtgat tatatctaat
      301 atttgtgcta tctttgatta actcgcgcat tttgcttacc aactcgggac gagttgggcc
      361 gggtgctggc tccttgcgcc tgaatggaac caccttctcg ctcatggtaa gcatcatatg
      421 ataggcgcgg cacgcctgtc aagatgacag gtaaaaatat atggatggaa ttgccgtagt
      481 ttcgataggg gaacccctaa tgccccgtta atgtgcagaa tgcggcagtc tgcacagcag
      541 attaggacac tccgccccta tttaggacag caccatgcgg ttgatgaact ggacctgcca
      601 gcccggtccc ggcgcctcac tggcgacgtg gtcgaggcgg gtgagcgaca ggttgtcggt
      661 gacgaaggcg agtgccgcgt cggcgtcgag cccgagcgcg tgcgcgatgg cggcgcggat
      721 cggcccgccg tgggcgacca ccaccacgtc gcgccccgca tgggcgcggg tgtggcggtc
      781 gatcgcggcg gcgacgcgcg ccatcaccgc aacgaagctc tcgccctcgg gcggggcgtg
      841 gtcggcggtg gtgtaccaga agcggtgccc gggcttgtcg gcgaggcgcg caacctgctc
      901 ataggtcagg ccctgccagc gcccgaacga ctgctcggcg aacgcgggct cgaccgcgac
      961 ggcggcgccg gcgaggccgc gggcgcggat cgccgaggcg gtatcgcgcg cgcgcctgag
     1021 cgcgctggtc agccacaccg cgtcctgggg cagcgccgcc gcgaaggccg cgaggcggtc
     1081 gccgtcgtcg agctcggccg gcgggtcgga ggcgccgtag agccggcccc gattcgcgat
     1141 cacccgcgcg tggcggaccc accaccaggc ggtgcgcacc tccttggcca tcggctcact
     1201 cgccgatctg cagcagcagg cccttgaccc gcgccatgcg catggtgccg aggaacgcgg
     1261 ccacgccaat gccgagatag accaggttga ggaggctcgc ctgggccagc aggtcgagcc
     1321 gcaggcggtg ctcgaccagc agcgcgcgca tgccctcgaa cacgtgtgcc gagggcaatg
     1381 cgcgcgcgac cggctgcagc cagtcgggca gcaccgccac cgggtagtag accccgctca
     1441 ccggctggat caggaacacc gccgcccagg ccaggctctc ggcgccctgg ccgaagcgca
     1501 gcaccatgcc gctgaccagc aggccgagcg cccagccgag cacgatcagg ttgacgaaga
     1561 aggcggcgag cggcaggccg aggtcgagga tcgagtagtc gaacatgacc gcggccagcg
     1621 ccgccgcgcc gcccaccccg atcaccgtgc gcaccaggct ggtggcgatg agcgccgcca
     1681 cgaactcgcc cgggcgcaag gggctgacca tcaggtggcc gaggttgcgc gaccacacct
     1741 cctcctcgaa gctcaggaac aggctgatgt tgccgcggaa caggatgtcc cacagcagca
     1801 ccgccgacag cagcacgccg gcggcctgcg ccacccaggt cgagttggtg atcaggaaca
     1861 gggtgatgaa tccccatagc accatctgca tggtcggcca gtagatcagg tcgagcaccc
     1921 gcggccacga cccgcgcatc aggtagaagt ggcgcagcat cagcgcgaag atgcgccgcc
     1981 acgactgcgc cgcaggatgc gcgctcatgc cgcgcccccg gcggcggcga ccgggtgcgg
     2041 gcggtcgcgg gcgatgtcga ggaacacctc ttcgagcgtg gcgcggccgt agcggtcgag
     2101 cagcgcgtcg ggcgcgccgc ggtcgatcac ccgcccggcc ttgagcatga tcacgtcggc
     2161 gcacagccgc tcgacctcgg ccatgttgtg cgaggcgagc aggatggtcg cgccgctggc
     2221 gcgctggtag cgctgcagat agccgcgcac gtagtcggcg atgtcggggt cgagcgacac
     2281 ggtcggctcg tcgagcagca gcagctcggg ctcgttcagc agcgctttgg ccagcgccac
     2341 ccgcgtcttt tggccggcgg acagcgtgcc caccttgcgc ttgagcagcg ggccgaggtc
     2401 gaggtcgcgc gccagttcgg cgatccgcgc cgcgatgtcg gcgagcccgt acaggtggcc
     2461 atagacggtc aggttctcgc gcacgctgag ccggtgcggc acctcgacat agggcgagga
     2521 gaagttcatc cgcggcagca cccggaagcg gtggcggagc acgtcctcgc cgagcacgcg
     2581 caccgccccc gcgtcgggca cgatcaggcc caagaggatg ccgatggtgg tggtcttgcc
     2641 ggcgccgttg ccgcccagca gcccgaccgt gcgcccgccg gcgatcgcga acgagacgcc
     2701 gtcgaccgcg accacgcgcc cgaaccgctt ggtcagaccg tcgacctgaa cgaccggctc
     2761 agccatccga ggggaagctc gtcacgcggg aagctcgtca ctggggcgcc aggggggccg
     2821 tggcgacccc tggctatcag cgcgcgccgg gccggtcaac ggccgccgcg caagcgcttg
     2881 taggcgatcg gcagcagcgc gagcagcgcc aggccggcca gcgccgcgat catggtcggg
     2941 gtgagcacgc ccgccaggct gaactccgcg cccgactcga gcacgctgcc gaggccggcg
     3001 ccggccaggc agtagacgaa ggtaccgggg atgatgccga agaaggtgcc gatcacgtag
     3061 gtgcgcaacg gcacctccag cagcgccggc accaggttga ccagccagaa cgggaacagc
     3121 gggatcaggc gcagcaccaa caggtagttg agcgcgttgt cgcggaagcc ctgttccatg
     3181 cgcctgagcg cgggcccggc gcggcgggcc agcacgtcgc cggcggcggt gcgcgcgatc
     3241 aggaagatca cggtcgcgcc cgcggtcgcc ccgatcaccg tgtacaaggt cgccagcgcc
     3301 gccccgaaca tgaagccgcc ggcaagggtc atgaaggtcg cccccggcag cgacagggcg
     3361 acgacgccgg catagaccgc catgtagccg atgcctgcga ccacgccgta gcgctggacc
     3421 agctcgagca gccgctcgcg gttggcgcgc aacgcctcga agctcagata gtcgtcgagg
     3481 ccgagcgcga aggcgagcgc gatcgccgcc gccagcaacg ccagcggcac aagccgacgc
     3541 cacagcggcc ggcgcccctc ggcggcctcg gtcaccgccg ccccggccgg tcgcggcggc
     3601 tactcgacgt cattcaggcg ccagtcgtag tcgtagccga ggatgcgcgg cgaggcggcc
     3661 agcgccgccg ccagcggcgg ctcgccgtag cggcgcaggt gcgcgaacag ctcggcgtcg
     3721 ctcgcgccga aatcgtcccg gtaccagtcg tagatgcgcg acaggcgcag cccgcgccca
     3781 gtcaccgtga ccccgcgcgg atggttgatg aaggcgcgcg cgctgtcctc gaggcaggcc
     3841 tcggcggtgt tcgccgtgaa cgcctcctta tagagctcgg gacagccgat cgaggcgcag
     3901 ttgagggcgt agtgcaggcg cgcgtcccgc cagatcgggc gcaggatgcg atgctcgatg
     3961 tcgtcgaggc tcacgccctc gccctcgacc gtgatcagct ctcgccccca ggggccgtcg
     4021 gcgaacagcc ccggcgagat gtcgatgtcg cggatcgagt cgaccggata gtggtcgagc
     4081 accacctgca cggtcagcgc gttgtagagg ttgatccagt aggcgagctg ctcggcgcgg
     4141 ccgagccggc tgaccggact cgccgcgagc gcatcgagat aggtcttgag cagcgccttg
     4201 tcctcgggcg cgacggcggc gtagtcgaag cgcgtcaccc cgtcggcgcc gggcctggtg
     4261 aagcgcgcga tcagccgccc ccaggcgccg tggtcgacct cgatcgtcga ggccggatcg
     4321 ttggccagcc agcggtccca cagctcggcc cggggcgccg ccgccgccgg cccgacgccg
     4381 gccgcgacgc acagcatcgc cccggcaaca acgcgcagcc acgcaccccg ggcgccgccc
     4441 ggcggctcag ccatgcgtgt gcgacctggc gcccccggtc acgatccgtc cggcttgttc
     4501 agccgccagt cgtagtagtc gccggcgatc gccgagtgct tcttcagctc cgcggcgagc
     4561 tcgggctcgg cgtatttcag caggtgctcg atcacgctct tgtcgttgcc gccgaagtct
     4621 tccttgaacc agctgtgata gaccttcgag gtgtacatct ggccgtcctc gaagcgcacg
     4681 ccctcggggc cgttgatgaa ctgatgcgcg atgcgctcca ggttctggcg ccagttctgc
     4741 gcggtgtgcg catgcggcgc aaggctcggg cagcccatcg aggcgcagtt cacgccatag
     4801 tggatcggcg gccccatgtc gttgaacgcg gcgcgcagga tatagtgctc gatgtcgttg
     4861 aagctcacga actggccgtg aacctgggcc accggcctca gccacggccc gaccacgttg
     4921 atccccgggc cgcggatctg gagcaccgag ttgaccggca tgctgtcgag caccaggtcg
     4981 acgatcgccg cgttgtagag gtccatccag aacgcgatct gctcgtcgcg actgtaggtc
     5041 tcgatctgga tggtcccgag ataggagaga tactgcttga gcgcgatgtg gtcgtcgtcg
     5101 ctgaccgcgt cgtagtcgat ccgattgacg ccgtcaggta gctcgcggtg gtatttctgc
     5161 aggaactctt cccaggggct gtgatcgatg gtctcggtcg agcccggatc gttgacgaac
     5221 cagaccgcgc gcgagtccgc gttcgagccg ggatcggtct ccgcctgctc ggtcgacggg
     5281 caggcggcga gcgcgggcgc catcatcgcc aacgtcacca ggcggatgca gcgttgggtg
     5341 gccatgtcct taaatatagg tctcgttgtc gcccatttcc gccgacgcgc cgccggcatc
     5401 cgggacgttc ccatgcgctg gtcatcgatc ctacccgcgc ccgatctgca gcgccactca
     5461 aacctgtgtg acctgcgccc cgagccgcta cactccgcgg ccgtggcggc aattgactcg
     5521 gtccggctca gcccgtatac agcggcgcaa ttccgcgctg tcgccgtgac ggagcctgtc
     5581 ggtgaaaagg acctatcaac cgagcgtcct ggtgcgcaag cggcgccacg ggtttcgcac
     5641 ccgcatggcg agcccgggcg gccggcgggt gatcgccatg cggcgcgcga agggacgcaa
     5701 gcggctatcg gcctgagcgg cacggcagcc tcgcgccgat tgcggcggct caagcggcgc
     5761 caggactttc tcagggttgc caggatcgga cgcaaatggg ccgcgccagg gctggtgctg
     5821 caatgcgccc cggcgctcgc cggaggcgcg ggcgtgccgg cggtcgggtt caccgccagc
     5881 cggcgcgtcg gcggggcggt ggcgcgcaac cgcgcccggc gccggctgcg cgccgcggct
     5941 gaccaggtca tcgccaccag tgcccgccct gacctcgatt acgtgctgat cgcccgcacc
     6001 gcgaccctgt cgcgccggtt cgaccggctg gcggcggacc tcatgaccgc gctcgccaag
     6061 ctcggcgcgc tcgccgagca ggccgcgggc gaggccccgg cggagcgccg gcaatgaccc
     6121 tggcggcgcg cgcgctcgcc gtgctggtcc acggctaccg gctgctggtg cggccgctgc
     6181 tgccggcctc gtgccggttc acgccgagct gctcggacta cgcgctcgag gcgctggcgc
     6241 gccacggcgc cgggcgcggg gcgctgctcg ccgcgctgcg actgtgccgc tgccacccgt
     6301 ggggcggctt cggctacgac ccggtgccgg acgacgcccg cgcctggcgc cggcgcggcg
     6361 agcgagcgtg agcggaggcc gcggtgcagg accagcgcaa cctgttgctc gccatcgtca
     6421 tctcggtcgc gatcctctac ggctcgcagc tgctgttccc gtcgccgccg cccgagccgc
     6481 cggcgccggc tgagaccacc ggcgcgccgc tgccggcgcc ggccgaggtc cctgcggccg
     6541 gcgtgccgac gccgccggcg ccggccgggg ccgaggcgcc ggcgcccgcc gccacgcccg
     6601 ccgggccgcg ggtgaccatc gagcgcggtg cccatggccg actgaccgga tcgatcgcgc
     6661 tgatcggcgg gcggctcgat tcgctcaccc tcgccgacta ccaggagacc gtcgatgcgg
     6721 acagcccgcc gatccggctg ctcgcgccgc cgggctcggc gcatccctac ttcgccgatt
     6781 tcggctggac cgcggacgcc gacgccggca tcgcgttgcc cgggccggac accccgtggc
     6841 gggcgagcgg cgccacgctc acgcccgaga cgccggtcac cttgagctgg gacaacggcg
     6901 ccgggctcac cttcgagcgc accgtggcgg tggacgacga tttcatgttc accgtcaccc
     6961 agcgcgtgcg caaccacggc gaggccgcgg tcacgcttta cggctacggc ctggtcagcc
     7021 gcaccgacga gcccaagacg ctcggctact acatcctgca cgaggggccg ctcggcgtgc
     7081 tcgacggcaa gctcaaggag gtcgactacg acgacctgcg cgaggcgctg accatcgacc
     7141 aaagcaccac cggcggctgg atcggaatca ccgacaagta ctggctcgcc gccctgatcc
     7201 ccgaccaggc gagcacggtg cggacgcgct tttccttcat ccgttcggac gggcgcgaca
     7261 agttccaggt cgactacctg ctgccggcgc tcgaggtcgc gcccggggcg tccgccgagg
     7321 tctcgagccg cctgttcgcc ggcgccaagg aggtgacgct cctggaccgc tacgaggagc
     7381 agctcggcat cgcccgcttc gacctcgcgg tcgatttcgg ctggttctac ttcctgacca
     7441 agccgctgtt ctacgtcatc cacacgttca gccgctggct cggcaatttc ggcctggcga
     7501 tcctgctgct gacggtgctg atcaagctcg tgttctaccc gctggcgaac aagtcctata
     7561 ccgcgatgag caagctgaag aag
//
LOCUS       DASWHU010000273         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1642565, whole genome shotgun sequence.
ACCESSION   DASWHU010000273 DASWHU010000000
VERSION     DASWHU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642565"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1975)
                     /locus_tag="VGB88_10125"
     CDS             complement(77..1975)
                     /locus_tag="VGB88_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9647846.1"
                     /translation="MTHALRTLALAWSAGGLLLAAVPSHAAGQPSASAACARAALGAG
                     AEAAAAAEGAHAMSMFGDVKYGPGFDHFDYADPDAPKGGEVRLLAIGTYDSLNPFILK
                     GVVAAGVATIYDTLLVGSADEPFTEYGLLVESIDMPADRSSVAFTLREGARWHDGEPV
                     TVEDVIWTFETLTTKGNPFFRQYYADVAKVEQTGPRTVRFSFSATTNRELPLIVGQIA
                     ILPKHYWQGRDFEATTLEPPLGSGPYRIAALDPGRSITYQRVPDYWGRDLAVNRGQDN
                     FDRMRYDYYRDATVAIEAFKAGEFDFKLENNSKDWATAYDMAEIAAGKVVKELIPNEL
                     PTGMQGFWFNTRRAKFADRQVRAALAYTFDFEWTNQNLFYGQYARTKSYFSNTELASS
                     GLPEGKELAVLDCYRGRVPEAVFTTPYEPPSTDASGTRRDNLRTAQQMLAEAGWRVER
                     GKLTGPDGQAMDIEFLLNAPAFERIVAPMVSNMKRLGIDARIRIVDPAQYELRKEEFD
                     FDVVVDSRGQSLSPGNEQRNQWTAAAAEVPGSDNIAGIKDPVIDELVDLVIGAADRES
                     LVAATRALDRVLLWGDYVVPNWHIRSFRIVYWNKFGRPETLPKYGIGFPATWWVDADK
                     ARALEARR"
BASE COUNT          293 a          715 c          783 g          341 t
ORIGIN      
        1 tcagcagcag ccggcggacg atgtaggcga gcatggcggg ctcacagctt ctcgagccgc
       61 gcgccgcgcg cgcccgtcac cgtcgcgcct cgagcgcgcg tgccttgtcc gcgtcgaccc
      121 accaggtggc ggggaagccg atgccgtatt tgggcagcgt ctcgggccgg ccgaacttgt
      181 tccaatagac gatgcggaag ctgcggatgt gccagttggg caccacgtag tcgccccaca
      241 gcagcacccg gtcgagcgcc cgcgtcgccg ccaccaggga ctcgcggtcg gccgcgccga
      301 tcaccaggtc caccagctcg tcgatcaccg ggtccttgat gccggcgatg ttgtcgctgc
      361 cgggcacctc ggcggcggcg gcggtccact ggttgcgctg ctcgttgccg gggctgagcg
      421 actggccgcg gctgtcgacg acgacgtcga agtcgaactc ctctttgcgc agctcgtact
      481 gggcggggtc gacgatacgg atgcgcgcat cgatcccgag ccgcttcatg ttcgagacca
      541 tcggtgcgac gatgcgctcg aacgcgggcg cgttgagcag gaactcgata tccatcgcct
      601 ggccgtcggg gccggtgagc ttgccgcgct cgacccgcca gccggcctcg gcgagcatct
      661 gctgggcggt gcgcaggttg tcgcgccgcg tgcccgaggc gtcggtcgag ggcggctcgt
      721 agggcgtggt gaacaccgct tccggcaccc gcccgcgata gcagtcgagg accgccagct
      781 ccttgccctc cggcaggccc gaggaggcga gctcggtgtt cgagaagtag ctcttggtgc
      841 gcgcgtactg gccgtagaac aggttctggt tggtccactc gaagtcgaag gtgtaggcga
      901 gcgccgcgcg cacctggcgg tcggcgaact tggcccggcg ggtgttgaac cagaagccct
      961 gcatgccggt cggcagctcg ttggggatca gctccttgac caccttgccg gcggcgatct
     1021 cggccatgtc gtaagcggtc gcccagtcct tggagttgtt ctcgagcttg aagtcgaact
     1081 cgccggcctt gaacgcctcg atcgccaccg tggcgtcgcg gtagtagtcg tagcgcatgc
     1141 ggtcgaagtt gtcctggccg cggttgacgg cgaggtcacg gccccagtag tcgggcacgc
     1201 gctggtaggt gatcgagcgg ccggggtcga gcgcggcgat ccggtaggga cccgagccga
     1261 gcggcggctc gagcgtggtc gcctcgaagt cgcgcccctg ccagtagtgc ttgggcagga
     1321 tggcgatctg gccgacgatc agcggtagct cgcggttggt ggtggccgag aacgagaacc
     1381 tgaccgtgcg cgggccggtc tgctcgacct tggcgacatc ggcgtagtac tggcggaaga
     1441 acgggttgcc cttggtggtc aaggtctcga aggtccagat cacgtcctcg accgtgaccg
     1501 gctcgccgtc gtgccagcgc gcgccctcgc gcagcgtgaa cgcgaccgat gagcggtcgg
     1561 cgggcatgtc gatcgattcg accagcagcc cgtactcggt gaacggctcg tcggccgagc
     1621 cgaccagcag ggtgtcgtag atggtggcca cgcccgccgc caccacgccc ttgaggatga
     1681 acggattcag gctgtcatag gtgccgatcg ccagcagccg cacctcgccg cccttgggcg
     1741 cgtcggggtc ggcgtagtcg aaatggtcga acccgggtcc gtacttgacg tcgccgaaca
     1801 tcgacatcgc gtgcgcgccc tcggcggcgg cggccgcctc ggcgccggcg cccagcgccg
     1861 cgcgcgcgca cgccgcgctc gccgacggct ggccggccgc atggcttggg accgcggcca
     1921 gcagcaggcc gccggcgctc caggcgaggg cgagggtgcg gagggcgtgc gtcatggctt
     1981 ggtcctcgat cgctggggcg gtgccgcccg ccggtgccgg tccctataag gtgcccgatg
     2041 cgcgccccag ttcaagggcg cgcgcacgat cgtgaccggc gtccgcgcgc tccctcagcc
     2101 gccccggccg gcgagatagg cgatcacggc gg
//
LOCUS       DASWHU010000274         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2742234, whole genome shotgun sequence.
ACCESSION   DASWHU010000274 DASWHU010000000
VERSION     DASWHU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742234"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGB88_10130"
     CDS             <1..634
                     /locus_tag="VGB88_10130"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9647847.1"
                     /translation="GLRAIRNLDGLVLTIPHKIAMAGLVDELAPNGRLVGAVNAVRCQ
                     ADGRWLGDMFDGAGCVNALARSGHRLRGRHVLQVGAGGVGRAIAFAFAGQGVARLTIN
                     DVAAERAAGLARAVAAAFPAVEAATGPARVHGHDTVVNCTPLGMAAGDPPPVPVADLA
                     PGMLVIDVVLLAVPTPLLAAAAARGCLTQTGRQMLDGQVTEIARFFGLGA"
     gene            complement(657..1055)
                     /locus_tag="VGB88_10135"
     CDS             complement(657..1055)
                     /locus_tag="VGB88_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9647848.1"
                     /translation="MTTVTPNFAHHTTLAALASRYVACDDLDWQPTRFPGIDVKVLMR
                     DEERGLMTALFRWAPGSFLPDHLHEDIEQTYVLTGTLEDDEGVAGPGDFVWRPPGSRH
                     DARSPNGAVMLSIFLQPNRFVGDEANGGTA"
     gene            1210..2679
                     /locus_tag="VGB88_10140"
     CDS             1210..2679
                     /locus_tag="VGB88_10140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9647849.1"
                     /translation="MYDLIIRNARIIDGTGAPARPGDLAVEGDRIAAIGDLAGARAGR
                     ELDAGGKALAPGFIDVHTHDDRALLSDPLMTCKVSQGVTTVVTGNCGISLAPWPPGHA
                     PVPPSDLIAERADQYFDSFAAYLAALDAEPPAVNVAAQVGHSSLRVGAMDRLDRAATA
                     REIAAMRARLEESLAAGAIGLSTGLFYRPANAAPTEEVIALAEAVAAAGGIHSTHMRD
                     ETDHITDSLAETFAIGRAAQVPVVISHHKCAGAPNHGRTRETLPMIAEARSRQPIALD
                     VYPYVAGSTVLDARRMMNARRVIVTWSRPHPEFAGRDLDDVAAELGLSIEAAAERLSP
                     AGAIYFIMDEDDVRRVLSFPHAMIGSDGLPHDRHPHPRLWGTFPRVLGHYVREVGLFD
                     LEEAVRRMTGLPAAQFGLVDRGALRPGTRADLVLLDPDTIADRATWERPTEPAAGIEM
                     VMVNGREVWRGGAHTGARPGRALRLQETGPKGRDTLIEQ"
     gene            2725..>2925
                     /locus_tag="VGB88_10145"
     CDS             2725..>2925
                     /locus_tag="VGB88_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647850.1"
                     /translation="MALAVAALGPAAADSVLKVVPHADLKNIDPIWTTAYITRNHGYM
                     VYDTLFALDEKLEVRPQMVERWE"
BASE COUNT          394 a         1133 c         1010 g          388 t
ORIGIN      
        1 cggcctgcgc gcgatccgca atctcgacgg cctggtgctc accatcccgc acaagatcgc
       61 catggcgggc ctggtcgacg agctcgcgcc caatggccgg ctggtcggcg cggtcaacgc
      121 cgtccgctgc caggcggacg gccgttggct gggcgacatg ttcgacggcg ccggctgtgt
      181 caacgcgctc gcgcgctcgg gccaccggct gcgcggccgc cacgtcctgc aggtcggcgc
      241 cggcggcgtc gggcgcgcga tcgcgttcgc cttcgccggc cagggggtgg cgcggctgac
      301 catcaacgac gtcgcggccg agcgcgctgc cggcctcgcg cgtgcggtcg cggcggcctt
      361 ccccgcggtc gaggccgcga ccggcccggc gcgcgtccac ggtcacgaca cggtggtcaa
      421 ctgcaccccg ctcggcatgg ccgcgggcga tccgccgccg gtgccggtcg ccgatctcgc
      481 gccgggcatg ctggtcatcg acgtggtcct gctggcggtg ccgacaccgc tgctcgccgc
      541 cgcggcggcg cgcggctgcc tcacccagac cggccgccag atgctcgacg gccaggtcac
      601 cgagatcgcg cgcttcttcg gcctcggcgc ctgagccgcg ccggcgccca gccggctcag
      661 gcggtgccgc cgttggcctc gtcgccgacg aaccggttgg gctggaggaa gatcgacagc
      721 atcaccgcgc cgttcggcga gcgtgcgtca tgccggcttc ccggcggccg ccagacgaag
      781 tcgcccgggc cggcgacgcc ctcgtcgtcc tcgagcgtcc ccgtgagcac gtaggtctgc
      841 tcgatgtcct cgtgcaagtg gtcgggcagg aacgaccccg gcgcccagcg gaacagcgcc
      901 gtcatcaggc cgcgctcctc gtcgcgcatc agcaccttga cgtcgatccc gggaaaccgg
      961 gtcggctgcc agtcgaggtc gtcgcaggca acgtagcgcg aggccaacgc cgccagcgtc
     1021 gtgtggtgcg cgaagttggg ggtcaccgtg gtcatcgatc cctccttgcg gcgcggccgc
     1081 caatacccgc ttcgccgcca ttgttccacc gccggcgccg atccgcaatc ccgctcgggc
     1141 aaggcgcgcg ccgtggcggc acccgatgtg ctacaagacc gcgacgccga cgccgtcccg
     1201 gacgccgcca tgtacgacct gatcatccgc aacgcccgca tcatcgacgg caccggcgcg
     1261 ccggcgcgcc cgggggacct cgcggtcgag ggcgaccgga tcgcggcgat cggcgacctc
     1321 gccggcgcgc gcgcggggcg cgagctcgac gccggcggca aggcgctcgc gcccggcttc
     1381 atcgacgtcc acacccacga cgaccgcgcg ctgctgtccg acccgctgat gacctgcaag
     1441 gtgagccagg gcgtgaccac cgtggtcacc ggcaactgcg gcatcagcct ggcgccgtgg
     1501 ccgccgggcc atgcgccggt gccgccgtcc gacctgatcg ccgagcgcgc cgaccagtat
     1561 ttcgactcgt tcgccgcgta tctcgcggcg ctcgacgccg agccgccggc ggtcaacgtc
     1621 gccgcccagg tcggccactc gagcttgcgc gtcggcgcca tggaccggct cgaccgcgcc
     1681 gcgaccgcac gcgagatcgc ggcgatgcgc gcccggctcg aggagtcgct cgccgccggc
     1741 gcgatcgggc tctcgaccgg cctgttctac cgcccggcca acgccgcgcc gaccgaggag
     1801 gtgatcgcgc tcgccgaggc ggtcgccgcc gccggcggca tccacagcac ccacatgcgc
     1861 gacgagaccg accacatcac cgactcgctc gccgagacct tcgccatcgg ccgcgccgcg
     1921 caggtgccgg tggtgatctc gcaccacaaa tgcgccggcg cgcccaacca cgggcggacg
     1981 cgcgagaccc tgccgatgat cgccgaggcg cgcagccgcc agccgatcgc cctcgacgtc
     2041 tacccgtatg tcgccggctc gaccgtgctc gatgcgcggc ggatgatgaa cgcgcgccgg
     2101 gtgatcgtga cctggtcgag gccgcacccc gagttcgccg gccgcgacct cgacgacgtc
     2161 gccgccgagc tcggcctgag catcgaggcc gcggccgagc ggctcagccc tgcgggcgcg
     2221 atctacttca tcatggacga ggacgacgtc cgccgcgtgc tgtcctttcc gcacgcgatg
     2281 atcggctctg acgggctgcc ccacgaccgc catccgcacc cgaggctctg gggcaccttc
     2341 ccgcgcgtcc tcggccacta cgtgcgcgag gtcgggctgt tcgacctgga agaggcggtg
     2401 cgccgcatga ccggcctgcc ggcggcccag ttcggcctgg tcgatcgcgg tgccttgcgt
     2461 cccggcacgc gcgcggacct ggtgctgctc gaccccgaca cgatcgccga ccgcgccacc
     2521 tgggagcgcc ccaccgagcc cgccgccggc atcgagatgg tcatggtcaa cggccgcgaa
     2581 gtgtggcgcg gcggcgccca caccggcgcc cggccggggc gcgccctgcg cctccaggag
     2641 accggcccca agggccgtga caccctcata gaacaatagg ttacgatgga tatgctgagg
     2701 aaaatactta gatcactctt cggcatggcg ttggctgtcg cggcgctcgg accggcggcg
     2761 gccgacagcg tgctcaaggt ggtgccccac gccgacctca agaacatcga cccgatctgg
     2821 accaccgcct acatcacccg caaccacggc tacatggtct acgacaccct gttcgcgctc
     2881 gacgaaaagc tggaggtccg cccgcagatg gtcgagcgct gggag
//
LOCUS       DASWHU010000275         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1644082, whole genome shotgun sequence.
ACCESSION   DASWHU010000275 DASWHU010000000
VERSION     DASWHU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644082"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGB88_10150"
     CDS             <1..267
                     /locus_tag="VGB88_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9647851.1"
                     /translation="NDEIAARLWHDLARALDLAPSPIAPWRVINERRATFAQTPDACR
                     RRPGARTSLANLVLAGDWTDTGLPATIEGAIRSGRRAAAVALGR"
     gene            449..>1825
                     /gene="dxs"
                     /locus_tag="VGB88_10155"
     CDS             449..>1825
                     /gene="dxs"
                     /locus_tag="VGB88_10155"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9647852.1"
                     /translation="MNTLSKTSLLDRIDTPDDLRALPETQLKQLADELRQELIDVVSH
                     TGGHLGAGLGVVELTVAIHYVFNTPRDRLIWDVGHQAYPHKILTGRRNRIRTLRQPGG
                     LSGFTKRGESEYDPFGAAHSSTSISSSLGMAVARDLSGGTNNVVAVIGDGAMSAGMAY
                     EAMNNAGSMESRIIVVLNDNDMSIAPPVGAMSAYLSRLISSHSYRSLRHVARQMARKF
                     PRPMAQAFRRAEEYARGMVTGGTLFEELGFYYVGPIDGHNIDHLLPVLKNVRDSQNGG
                     PVLVHVVTEKGHGYPPAVQSADKYHGVSKFDVVTGAQAKKKGNAPSYTSVFARALIEE
                     AKRDDKIIAITAAMPSGTGLDKFAEAFPERCFDVGLAEQHAVTFAAGLASEGYKPFAA
                     IYSTFLQRAYDQVVHDVAIQRLPVRFAIDRAGLVGADGQTHAGSFDVTYLGCLPGFVV
                     MAAADEAEL"
BASE COUNT          303 a          656 c          594 g          272 t
ORIGIN      
        1 aacgacgaga tcgcggcccg cctgtggcac gatctcgccc gcgccctcga cctcgcaccg
       61 tcgccgatcg cgccgtggcg ggtgatcaac gagcggcgcg cgaccttcgc ccagaccccg
      121 gatgcctgcc ggcgccggcc gggcgcgcgc acctcgctcg ccaacctggt gcttgccggc
      181 gactggaccg acaccggctt gcccgcaacc atcgagggcg cgatccgctc gggccggcgc
      241 gccgccgcgg tcgcgctcgg ccgctgagcc cggtcacggc cgatcggcgc aggcgcgtcg
      301 cacgggccgt gacaattttg tcacaaattg gcaatcgaat tgatcggacg ggctttagca
      361 gctatctata accttagatt cgttttcaaa aatcacgttg agaaaggcga ttcacctgcc
      421 gcccccgtac cgatcgagga gtggacccat caacacactc agcaagacct cccttctgga
      481 ccgtatcgac accccggacg acttgcgggc cttgcccgag acgcagctca agcagcttgc
      541 cgacgagctg cgccaggagc tgatcgacgt cgtctcgcac accggcggcc accttggcgc
      601 cggcctcggc gtggtcgagc tcacggtcgc gatccactac gtgttcaaca ccccgcgcga
      661 ccgcctgatc tgggacgtcg gccaccaggc ctacccgcac aagatcctca ccggccggcg
      721 caaccgcatc cgcaccctgc gccagcccgg cggcctgtcc gggttcacca agcgcggcga
      781 gagcgagtac gacccgttcg gcgcggcgca ttcatcgacc tcgatctcgt cgagcctggg
      841 catggcggtg gcgcgcgacc tgtcgggcgg caccaacaac gtggtcgcgg tgatcggcga
      901 cggcgcgatg agcgccggca tggcctatga ggcgatgaac aacgccggct cgatggagag
      961 ccggatcatc gtcgtcctca acgacaacga catgtcgatc gcgccgccgg tgggggcgat
     1021 gagcgcgtat ctgtcgcgcc tgatctcgtc gcacagctac cgcagcctgc gccacgtggc
     1081 gcgccagatg gcgcgcaagt tcccgcgccc gatggcccag gcgttccggc gcgccgagga
     1141 atatgcgcgc ggcatggtca ccggcggcac gctgttcgag gagctcggct tctactatgt
     1201 cggcccgatc gacggccaca acatcgacca tctgctgccg gtgctgaaga acgtccgcga
     1261 cagccagaac ggcggcccgg tgctggtcca cgtggtcacc gagaaagggc acggctaccc
     1321 gccggcggtg cagtcggccg acaagtatca cggggtcagc aagttcgacg tggtcaccgg
     1381 cgcccaggcc aagaagaagg gcaacgcgcc gagctacacc tcggtgttcg cgcgcgcgct
     1441 gatcgaggag gcgaagcgcg acgacaagat catcgcgatc accgcggcga tgccgtccgg
     1501 caccggcctc gacaagttcg ccgaggcatt ccccgagcgc tgcttcgacg tgggccttgc
     1561 cgagcagcac gcggtgacct tcgccgccgg gctcgcgagc gaaggctaca agccgttcgc
     1621 ggcgatctat tcgaccttcc tgcagcgcgc ctacgaccag gtggtccacg acgtcgccat
     1681 ccagcgcctg ccggtgcgct tcgccatcga ccgcgccggc ctggtcggcg ccgacggcca
     1741 gacccatgcc ggatcgttcg atgtcaccta tctgggctgc ctgcccggct tcgtggtgat
     1801 ggcggccgcc gacgaggcgg agctg
//
LOCUS       DASWHU010000276         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3462309, whole genome shotgun sequence.
ACCESSION   DASWHU010000276 DASWHU010000000
VERSION     DASWHU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462309"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1125)
                     /locus_tag="VGB88_10160"
     CDS             complement(13..1125)
                     /locus_tag="VGB88_10160"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX9647853.1"
                     /translation="MRRLPIPAPVPRRTPLGVALAALLAVTLDSGALAHDIGISNVRV
                     EVLEDGAETQLAVKGRDLDRALGTAIVDPAADQVDGSALAAAAGQIIDHVLARVSVAQ
                     ADGVACTATADPPQTDADGVVLWLAWHCEDAGRGLVYGNHLFHEFNPRAIQNVLILKG
                     DDVEQSVLTSYRTELSLTEAPPSYVEVAGRYIRTGIEHIFLGYDHIAFLIALLLWAKR
                     LWPVVKVVTAFTIAHSITLALAVLDIVSLPASVTEPLIAVTIIWVAVENFLSRDVGRR
                     WRIAFLLGLIHGFGFAGVLRAFGLPSEALGLALASFNIGVEIGQVGIVAIAVPVLLAI
                     DRIRGPEARRAVVVHAASLTIGALGLFWLVERTVNA"
     gene            1296..3569
                     /locus_tag="VGB88_10165"
     CDS             1296..3569
                     /locus_tag="VGB88_10165"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX9647854.1"
                     /translation="MTSVPTSASPPASHALSDRRGAATLALFAATLFVGAGLVFWIQP
                     LFTKMVLPMLGGSPSVWNTAMVFFQAGLLAGYAYAHLLSRYLPPAWQLRIHLIVLAAA
                     FLFLPVSVTGGWRPDSDAAPVIWLLALMTVTLGLPFLAVSATAPLVQRWFSRTGHAHA
                     EDPYFLYAASNLGSVLALVGYPLLFEPWLTGPAQSLAWTVGYVIAAVLIALAGVVMLR
                     SHDAAPDVRSAARAAGALARPGWRLRLRWLVYAAVPSAMLLGVTAHITTDVAAAPLFW
                     VIPLTLYLFTFVIAFSRRPLVPHRIAVRALPIALVLLVSLFTWHAPVGLVIAVHLAAF
                     FITALACHGELARMRPHVDALTEFYLFLSIGGMLGGMFTALLAPVVFDGVYEYPLAIA
                     AACALLPGDRRAARPADGVLALVLLAILVAATRLGPAVSGLNPVQVTAAVAPVLAFIA
                     FTRRGRPFAFALALAAVLAGTMLSFERPGTLWRERSFFGVYRVVETENAAARLLIHGT
                     TIHGAQLQAADLRYVPASYYGPGTPIADLIAKARARRGSLRTGVVGLGAGGLACHRAK
                     AEDWRFYEIDPLVAAVARDGRYFDLLAGCAPGGAVVLGDARLSLVDEPATSFDLLIMD
                     AFNGDAPPIHLITREALELYLDKLAPGGLVAVHISNRFVDLRGVIARLVARLGLAARV
                     GTTAFADAPRTDAGHYATAMTVWVVIARDPAELDALGLGDQWHALPAADGGRAWTDDY
                     SNILEAIRWRGTDFTVR"
BASE COUNT          497 a         1455 c         1282 g          619 t
ORIGIN      
        1 cggccggccg catcacgcgt tcacggtccg ctcgaccagc cagaacaggc cgagcgctcc
       61 gatcgtcagc gacgccgcat gcaccacgac cgcgcgccgc gcctcggggc cgcgaatccg
      121 gtcgatggcc agcagcaccg gcaccgcgat cgcgacgatc ccaacctggc cgatctcgac
      181 gccgatgttg aacgacgcca gcgccaggcc gagcgcctcc gacggcagcc cgaacgcgcg
      241 cagcacgccg gcgaatccga agccgtggat caggcccagc aggaaggcga tgcgccagcg
      301 ccggccgacg tccctggaca ggaagttctc gaccgcgacc cagatgatgg tgaccgcgat
      361 cagcggctcg gtcaccgacg ccggcaggct gacgatgtcg agcaccgcca gcgccagcgt
      421 gatcgaatgg gcgatggtga acgcggtcac caccttgacc accggccaca gccgcttggc
      481 ccacagcaac agcgcgatca ggaacgcgat gtggtcgtag ccgaggaaga tgtgctcgat
      541 gccggtgcgg atgtagcggc ccgcgacctc gacatatgac ggcggcgcct cggtcagcga
      601 cagctcggtc cggtacgatg tcagcaccga ctgctcgacg tcgtcgccct tcaggatcag
      661 cacgttctgg atcgcgcgcg ggttgaactc gtggaacagg tggttgccgt agaccagtcc
      721 gcgaccggca tcctcgcagt gccaggccag ccacagcacc acgccgtccg cgtcggtctg
      781 cggcggatcg gcggtcgcgg tgcaggccac gccgtccgct tgcgcaaccg acacgcgcgc
      841 cagcacatgg tcgatgattt gcccggcggc cgcggccagg gccgagccat cgacctggtc
      901 ggcggccgga tcgacgatcg cggtgccgag cgcccggtcg aggtcgcggc ccttgaccgc
      961 gagctgcgtc tcggcgccgt cctccagcac ctcgacgcgc acattgctga tgccgatgtc
     1021 gtgggcgagc gcgccgctgt ccaaagtaac ggcaagcagc gccgcgagcg cgaccccgag
     1081 cggggtccga cgcgggacgg gtgccggaat ggggagccgt ctcacgtcgc cgtgtcctcc
     1141 ctgcggggcg cgagattggc atgatcggcg cggcctcgcc aaggcccgac ccgaccgggc
     1201 tgtgcggccg cgcttagccc gatcgtcgat ccgccctatc ttcccaagac ggaatcgctc
     1261 gcccagcact gccgcatgac gccacggacc gcctgatgac cagcgtgcca accagcgcca
     1321 gcccgcccgc ctcgcacgcg ctgagcgacc gccgcggcgc cgcaaccctg gcgttgttcg
     1381 ccgcgaccct gttcgtcggc gccggcctcg tgttctggat ccagcctctg ttcaccaaga
     1441 tggtgctgcc gatgctgggc ggttcgccgt cggtgtggaa cacggcaatg gtgttcttcc
     1501 aggccggcct gctcgccggc tacgcctatg cgcatctgct cagccgatat ctccccccgg
     1561 cgtggcagct ccgaatccac ctgatcgtgc ttgccgcggc gttcctgttc ctgccggtgt
     1621 cggtgaccgg cggctggcgg ccggacagcg acgcggcgcc ggtgatctgg ctgcttgccc
     1681 tgatgaccgt caccctcggg ctgccgttcc tcgccgtctc cgcgaccgca ccgctggtcc
     1741 agcgctggtt ctcgcgcacc ggccacgcgc acgccgagga tccctatttt ctctacgccg
     1801 cgagcaatct cggcagcgtg ctggcgctgg tcggctatcc gctgctgttc gaaccctggc
     1861 ttaccggtcc tgcccagtcg cttgcctgga ccgtcggcta cgtgatcgcg gccgtgctga
     1921 tcgcgctcgc cggcgtggtc atgctgcgca gccacgacgc cgcgcccgat gtgcgctcgg
     1981 cggcgcgtgc ggccggcgca ctcgcgcggc ccggctggcg cctgcggctg cgctggctgg
     2041 tttacgccgc agtgccgtcc gcgatgctgc ttggggtcac cgcccacatc accaccgacg
     2101 tcgccgccgc gccgctgttc tgggtgatcc cgctcacgct ttatctgttc accttcgtca
     2161 ttgccttctc gcgccggccg ctggtcccgc accggatcgc ggtgcgcgca ttgccgattg
     2221 cgctggtgct gctggtcagc ctgttcacct ggcacgcgcc ggtggggctg gtgatcgcgg
     2281 tccatctcgc cgccttcttc atcaccgcgc tcgcctgcca cggcgagctg gcgcgcatgc
     2341 gtccccatgt cgacgcgctg accgagttct acctgttcct gtcgatcggc ggcatgctcg
     2401 gcggcatgtt caccgcgctg ctcgcgccgg tcgtcttcga tggcgtctac gagtatccgc
     2461 tcgccatcgc cgccgcctgc gcgctgctgc cgggcgaccg ccgggcggcg cggcccgccg
     2521 atggcgtgct ggcgctggtg ttgctcgcga tactggtcgc ggcgacccgc ctcgggccgg
     2581 ccgtcagcgg cctcaatccg gtccaggtga cggcggcggt ggcgccggtg ctggcgttca
     2641 tcgcctttac ccgcaggggc cggccattcg ccttcgcgct ggcgctcgcc gccgtgctcg
     2701 ccggcaccat gctgtcgttc gagcgcccgg gcaccttgtg gcgcgagcgc agcttcttcg
     2761 gcgtctaccg ggtggtcgag accgagaacg ccgccgcccg cctgctgatc cacggcacca
     2821 ccatccacgg cgcccagctc caggcggccg acctgcgcta cgtgccggcc tcctattacg
     2881 gccccggcac gccgatcgcc gacctgatcg ccaaggcgcg ggcgcgccgc ggaagcttgc
     2941 gcaccggcgt ggtcgggctc ggcgccggcg gcctcgcctg ccaccgcgcc aaggctgagg
     3001 actggcggtt ctacgagatc gacccgctgg tcgcggcggt cgcccgcgac ggccgctact
     3061 tcgacctgct cgccgggtgc gcgcccggcg gcgccgtcgt gctcggcgac gcccgcctgt
     3121 cgctggtcga tgagcccgcg acctcgttcg acctgctgat catggacgcg ttcaacggcg
     3181 acgcgccgcc gatccacctg atcacccgcg aggcgctcga gctctacctc gacaagctcg
     3241 ccccgggcgg cctcgtcgcc gtccacatct ccaatcggtt cgtcgacctg cgcggcgtca
     3301 tcgcccggct ggtcgcccgg ctcggcctcg ccgcgcgcgt cggcaccacc gccttcgccg
     3361 acgccccgag gaccgacgcc ggccattacg ccaccgcgat gaccgtgtgg gtggtgatcg
     3421 cgcgcgaccc ggccgagctc gacgccctcg gcctcggcga ccagtggcac gcgctgcccg
     3481 ccgccgacgg cggccgggcg tggaccgacg actactccaa catcctcgag gcgatccgtt
     3541 ggcgcggcac cgacttcact gtccgctagc ggcagctgga gcgggcgacc gggattgaac
     3601 cggcgacatt cagcttgaga agttggttca gccggacgcc gacgtggaca ctccgggatg
     3661 aaacggtata catctccgag ctgtgcgagc gtcgccgtcg cggttagctc gccactatgt
     3721 gcacatccgg ttcgagtcgg ttcgtaaagg tgctccgagg gcgaacgcta ccgcctcgtg
     3781 cagggtcatg ccatgcccac gcgcgctggc ctcctccacc gcgtcacggc tcaatccggt
     3841 gccggcagct tcc
//
LOCUS       DASWHU010000277        14966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1645520, whole genome shotgun sequence.
ACCESSION   DASWHU010000277 DASWHU010000000
VERSION     DASWHU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645520"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGB88_10170"
     CDS             complement(<1..327)
                     /locus_tag="VGB88_10170"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEX9647855.1"
                     /translation="MNVVDAARGYLARGWSVIPIEARGKRPLVRWQRYQQHLAEPDEI
                     ARWFAADADANVGIVTGRLSRLVVLDVDPGHGGADSIAAWERRHGPMPETVECTTGGG
                     GRHLYFG"
     gene            466..1188
                     /locus_tag="VGB88_10175"
     CDS             466..1188
                     /locus_tag="VGB88_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9647856.1"
                     /translation="MTTTQQVLDRARHALASEPRLDLHRFPLSLAFDGQTLTIEGEVG
                     DIAAKKLALERAAALPEVAGIIDRLRVQPAQPMGDREIRDHLANAFQGEPAFTDLELV
                     EKDGERTLVLHRPDEAAGRIEFAAADGVVTLDGRVPTLARKRLAGVLAWWVPGSRDVV
                     NGIAVEPPEEDSPDAIEDAVALVLDRDPFVDGSQIRVGVRHAVVHLTGLVPTESERDM
                     AEFDAWYVFGVDKVINDIEVRA"
     gene            1221..2033
                     /locus_tag="VGB88_10180"
     CDS             1221..2033
                     /locus_tag="VGB88_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX9647857.1"
                     /translation="MADADCTSFLQDVLPRLGLRWPGFRKVRGQVCKRIRRRMAALGT
                     PDFAAYHRHLDSRPEEWAVLDSLCRVSVSRFWRDRAVFDHLRRSLLPRLAAAAAERTP
                     PRARLWSAGCASGEECYSLALAWQLGAAGRTATLVEIVATDADHALIARALGGCYPPS
                     SLADLPTDICRAGFVRAGEEWCVRTRFRIGIRFLCQDIREAMPPGPFDLVLCRNLAVT
                     YFDEATRRRVLAAIAASMRPGGSLVVGAHEQLARAGPEFVPDPGDFPIYHRV"
     gene            2068..2763
                     /locus_tag="VGB88_10185"
     CDS             2068..2763
                     /locus_tag="VGB88_10185"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9647858.1"
                     /translation="MARSPSSGRGAEAETHERRAMIREIEAEARETARYTGRSAFDAR
                     IMAALAEVPRHEFVPAGERDFAYFNEPLPIGHGQTISQPYIVALMTDLLEPQPEHVVL
                     EVGTGCGYQAAVLSRLVRRVYSIEVVAALAASARERLARLGFDNVEVRAGDGAAGWPE
                     HAPYDGIVVTAAAAELPPALVHQLKPGGRMVIPLDSGLWQDLKLVIKDDKGGIDSRTV
                     LPVAFVPLTHPGK"
     gene            2777..3871
                     /gene="rtcA"
                     /locus_tag="VGB88_10190"
     CDS             2777..3871
                     /gene="rtcA"
                     /locus_tag="VGB88_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX9647859.1"
                     /translation="MAADAWLEIDGGHGEGGGQIVRSALSLAAVTGRAVRILRVRAGR
                     PRPGLAAQHVTAARAVAAVCAGRLDGDRLGSQSLALEPTAPPRAGDYLFDVAAARTGG
                     SAGAVSLVLQATLLALAFASGRSTLQLHGGTHMAWSPSFDYCRDVWLPALAEMGIAGE
                     AELRAWGWFPVGRGEVHARIMGAGPGFRPRSVDWSARGGLVAVRGRAVAANLPAHIPQ
                     RMCDRARALLAEAGIESRLTPVRVVAACPGAGLFLTAEYERGRAGFTALGERGKASET
                     VAEEAVAELLAHHGSGAALDLHLADQMVLPAALADGESRYTAARVSGHLETMAWLVER
                     FGLARVEIAPAARDAPALITVRPRSQSSQE"
     gene            complement(3853..4293)
                     /locus_tag="VGB88_10195"
     CDS             complement(3853..4293)
                     /locus_tag="VGB88_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEX9647860.1"
                     /translation="MSDDYGPGYGDDGDRDEQVVGIAIEKVCALITHVRAFDAKVEIA
                     DPDFAAGLSEADMISVHESFANDPVYEELVGFIDALNEEEQVNLVALTWLGRGDFSAE
                     EWEDALHEARAARSDHTDTYLLGIPLLGDYLAEGLAQLGYSCDD"
     gene            complement(4310..4558)
                     /locus_tag="VGB88_10200"
     CDS             complement(4310..4558)
                     /locus_tag="VGB88_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647861.1"
                     /translation="MEIRVDCYAGHRGEETPRCLHMGARAIEVSEVVDRWLAPEHSYF
                     KVKGDDGALYILRHDEMDGRWDLTMYDRGGTLEAPATR"
     gene            4713..5183
                     /locus_tag="VGB88_10205"
     CDS             4713..5183
                     /locus_tag="VGB88_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX9647862.1"
                     /translation="MQRFRCDRMLARLGRFLRAAGYDTEIADDGADDRDLVARAASEA
                     RVLLTCDRAIAQHAAARGAVILPANGVDAQARALAGALDIDWEMAPFTRCLIDNARLR
                     TARADEIARLPARSRQLGGPFRVCPACARLYWPGSHVRRMRARLARWSTEIRDT"
     gene            5481..6584
                     /locus_tag="VGB88_10210"
     CDS             5481..6584
                     /locus_tag="VGB88_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9647863.1"
                     /translation="MRTVVGTVIGLVVGAVVGVFVLAPTSVERGEPAPGEQAGLTTAA
                     AAPVHWKMPSSFAGEMVQLGTLGRRFETRLMEASGGTMDVKFYEPGALVPALEIFDAV
                     STGAVDAGWSSPGYWAGKIPALQFFAAVPFGPDPGEYLAWLYYGGGKELFEEIYAPHN
                     IHPLHCGMISPEGSGWFRNEITSLDDLKGLKMRFFGLGAKVMEKLGVSTQLLAGGDIF
                     PALELGTIDATEFSMPAIDLNLGFYQVAKHYYFPGWHQPASLIELIINMDRWNALSDA
                     QRAMVEVTCGDSIREGIAEGEAIQFKALEELQSKGVILHRWSPEIMDALRTAWAEVVA
                     EESAKDADFKRVWESLDGFRQGYALWRSYGRLD"
     gene            complement(6636..12422)
                     /locus_tag="VGB88_10215"
     CDS             complement(6636..12422)
                     /locus_tag="VGB88_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9647864.1"
                     /translation="MGIARGDWEARSIGLYLRAGVAGIAGLTVLVAVVALSAFVMFSI
                     NVGSVAGTAVPALTQANELRRAADKVDRSARNLANLDGAFAVQSGILGLRADAGQLAE
                     SIDKLDESVASTGEAEELNASMQLLTGVLERLESLVERRIALDDALAERAARLAALQR
                     RIEKFAWSGEASDDRRQAAAVPMMRAAGAQLAAIASDDPKEVDELKASFDRQFEQARQ
                     KVDGLQDEGSVAPGRLLAEMLLVEIKTAADVFDQRIERLLLDLRFQGALRRMAVMDRL
                     AATAAGIADRLSSAALAASADAEGAIALPSYLLIGMSLIGVLGAVALAGFVDRRVVTR
                     IKRLHRAMTAHDSGAREGIEVDGRDEIADMARSFASVVDAADQKDSSFTQRLRELDHV
                     RKTLADQDTVIHAAVDNLPDGIFVLDRNLRFRLFNRRCATLLGVKPDLLGLGEPVRPV
                     MVEAARAGLFGPGDEDELVEQCLADFASQEPAESELRASEGRILSARKMPLRDGGLVV
                     VLADVTEHKRCLEGLARAEEQNGTLIANLPGAVYRLALDGQWTVDFVSDAVERLSGRP
                     AGDFIGRPASGFAQMIHAEDRQKVRDVLVQAAQAGARYDVEYRIVRGEGEVRWIAEQG
                     WVSPAEGDAPAYLDGILLDVTDRKTAEAKLAERTRVLDAVLAGVDQGVSLCDEALEAV
                     AFNDKFLDLAEVPKDRFAIGDSLEAFIRFSAERGDYGPGDVERMVRERIGLAKSFEPQ
                     QAERVRSDGTVVEIRSRRIPGIGHLTTCSDVTVLRRAEQQAGDGERRLRAILEHCPVG
                     VAITRIDDGSYIFANRRYVEDNGYEDTDGVIEGTAIERYADPDQRADIIAQLEREGAV
                     RDIEVLRRRCNGSRYWDLMSVYRHQEDGVDTLLSWHTDITERKRAELEVKRSRESLDQ
                     ILASSPAGISIIDRQSRQRMYVNDRYLQLFGAESEAQLASQDIADSFVERADLQRVLA
                     CLESQERQAPVEVERRRFDATTWWSLIDHDRVFYQGREALILWHYDITDRKRAEERLG
                     FSERRLRHMLEGCPIGTVVVRTGDGTVASCNAPYAGMVGEEVERVVGRPGPQSILDAV
                     PAGGSACNLEAQLETAGAEPLSVLVSVVPTETQDGEPARLCFVRDVTGLEEAERQAAL
                     AGARIRSVLDATAEGVWMIDPDGRIVDANPAICRILGKPREALLGQRPDSFTDEAGRD
                     IVGRSARSEVTLVRPDGGTVACLASASDYADADGKIVGTVVLCADISDLKRHEQRLRR
                     SETRLRRVLEAAPVGIGIARVSDGSQIYVNAALARLLAAEADHLIGRQADEPWVEREA
                     WQDFVESWYRDGEVAGLEARLRSSDGGERRVVASAMAEVETDDGEPARFVWYTDVTDR
                     KIAEEQLRDTQARLDEGEERLTQSGQQLQQAHAQIGESAERLSQREARLHETENRLRE
                     SVERLTQSEEDLRETRSRLSQGEQRSSRSEEQLRETRAQLSRTAERLSQSEQQLHQMQ
                     AQVRQSGERLARSEDQLRQARARVSQDEERMRETRAELSQVKERLSDSETRLNRSGDR
                     LRAILDGSPIGALIVRASDGRVTFANRRMAEMLGRAPEEIAGGDARRYGFEPAAGDAA
                     PPPWRADGSFDESDLRLHRADGSELWVRRAARPLTTEAGEAARLSWYDDISHRKHMEA
                     ELSEAKQAAAAGSGAKAELLASVSREMRRPMTGIVGMADLLWKTDLDGEQRRMLGNVR
                     DSGRACLGVINQFRDLSKIEAGELQLEPAPTSIEAVVEGSAAMLAAEARCKGVQLVTR
                     VAPDIPPRVLADPVRLRQIVVNLVGNALDFADAGDIVIHAARAGSDDRGRERIRIEVV
                     DQGPSQPTTEDGPSTERDRNHADGGLRLSICRRLAELMGGEIGIDGNPGEGTTVHVEL
                     PLPRADAGPTPRAQPTQTAPVPSPERAL"
     gene            complement(12472..13557)
                     /locus_tag="VGB88_10220"
     CDS             complement(12472..13557)
                     /locus_tag="VGB88_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9647865.1"
                     /translation="MTMTADGVPSRDRLRAALARCRFLVLLCALMTPYSAQALSPAVL
                     FATADVEGDGSARLALDGARSFGRMRGIEVAILGPPAGEDIDASAARDLVTGAIEAGH
                     DIVVGVGPVFAESFRQLAPEHQAVRFVLVDAVVDLGNVESIEFNVEQGSFLVGALAAM
                     ASKTGKVGFVGGRDGALARTFACAFFEGARRLNPRIEVVSAMAGDTDPALADAGKGTE
                     LVRTLAEGGVDVVYHAAGASGDGIIAAAARQGILAIGAGGNQNGLAPGHVLTSMIKRL
                     DVAVFAALSDIADGAWQPGTRRLGLRDGGVDWALDRHNVLLIDDDMQRRIETLTFEAI
                     RGKIEVAAYSSRGGCPDFDFGPLPGTD"
     gene            complement(13681..14142)
                     /gene="queF"
                     /locus_tag="VGB88_10225"
     CDS             complement(13681..14142)
                     /gene="queF"
                     /locus_tag="VGB88_10225"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX9647866.1"
                     /translation="MTERFDRLTQLGRARAIPASPEQAELEKVANDHVDVDYLARFTC
                     PEFTSLCPVTGQPDFATFVIDYVPDRWILESKSLKLYLHAFRNHAAFHEDCTVAIGKR
                     IVVAVAPRWLRIAGFWNPRGGIPIDVVWQTGPAPAGLYVPDPGVPPYRPRG"
     gene            14293..>14966
                     /locus_tag="VGB88_10230"
     CDS             14293..>14966
                     /locus_tag="VGB88_10230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9647867.1"
                     /translation="MDVGLFCLLNQRDKSQAPRQVIAETVEQVRLADRIGLSIAWFAE
                     HHFSNYCLCASPLLMIGHCAAVTSRIRLGTGVLVLPLYAPMRLLAEIGFADCLSGGRL
                     VLGIGSGYQDYEFERFGTDLAESKERTLELLDMIERSLATGTVDHDGRYYTQPVTRLS
                     VTPLQRPHPDIWIAGAAPALMQRAARSGYPVIVTQRLSGVDFLVSGREWCARAYRDAG
                     VDPARM"
BASE COUNT         2066 a         5403 c         5183 g         2314 t
ORIGIN      
        1 cccgaaatag aggtggcggc cgccgccgcc ggtggtgcat tcgaccgtct cgggcatcgg
       61 accgtgccga cgctcccagg cggcgatgct gtccgccccg ccatgccccg gatcgacatc
      121 gaggaccacc agcctggaga ggcgaccggt gacgatgccg acattggcgt cggcatcggc
      181 ggcgaaccag cgcgcgatct cgtccggctc ggccaggtgc tgctggtagc gctgccagcg
      241 caccagcggg cgcttgccgc gcgcttcgat cgggatcacc gaccagcctc tggccaagta
      301 accgcgcgcg gcgtcgacaa cgttcatccc gccctcctcc atgtgccgtg cgcggcagcc
      361 gcgcgcgcgg cgacaccgga aatcgaagcg aaccacagaa accttggcta tgctaaccgg
      421 ctacgcgcgc tcggccccga ccggaacaat ctcggcagcc aatcgatgac gaccacgcaa
      481 caggttctcg accgtgcgcg ccacgcgctg gccagcgagc cgcgcctgga cctgcaccgg
      541 ttcccgctgt cgctcgcctt cgacgggcag actctgacca tcgagggcga ggtcggcgac
      601 atcgccgcca agaagctcgc cctcgagcgc gcggcggcgt tgcccgaggt ggccgggatc
      661 atcgatcggc tgcgggtgca gccagcgcag ccgatgggcg atcgcgagat tcgcgatcat
      721 ctggcgaacg cgttccaggg cgagcccgcc ttcaccgacc tggagctggt agagaaggac
      781 ggggagcgga cgctggtgct gcaccggccg gacgaggccg caggccggat cgagttcgcg
      841 gccgccgacg gcgtggtcac gctcgacggc cgggtgccga cgctggcccg caagcggctc
      901 gccggggtgc tcgcctggtg ggtgcccggc agccgcgacg tggtcaacgg catcgcggtc
      961 gagccgcccg aggaggacag ccccgacgcg atcgaggatg cggtggcgtt ggtgctggac
     1021 cgcgacccgt tcgtcgacgg cagccagatc cgggtcggcg tgcgccacgc cgtggtccac
     1081 ctgaccgggc tggtgccgac cgagtccgag cgcgacatgg ccgagttcga cgcctggtac
     1141 gtgttcggcg tcgacaaggt gatcaacgac atcgaggtgc gcgcctgaga cgcgttccgg
     1201 gcgcgtccca tgacgccgcg atggccgacg cggattgcac gagcttcctg caggacgtgc
     1261 tgccgcgtct gggcttgcgc tggccgggct ttcgcaaggt gcgcgggcag gtgtgcaagc
     1321 gcatccgccg gcgcatggcc gcgcttggca cgccggattt cgccgcctac caccgtcacc
     1381 tcgactcccg gcccgaggag tgggcggtgc tcgattcgct gtgccgggtg tcggtgtcga
     1441 ggttctggcg cgatcgggcg gtgttcgacc atctgcgccg gtcgctgctg ccgcgccttg
     1501 cggccgcggc ggccgagcgc actccgccgc gcgcgcggct ctggagcgcg ggctgcgcct
     1561 cgggcgagga atgctactcg ctggccctgg cctggcagct cggcgcggcc gggcgcaccg
     1621 ccaccctggt cgagatcgtc gccactgacg ccgaccacgc cctgattgcg cgcgcgctgg
     1681 gcgggtgcta tccgccttcc tccctcgccg acctgcccac cgacatctgc cgcgcgggat
     1741 tcgtacgcgc gggcgaggag tggtgcgtgc gcacccggtt ccgcatcggc atccgcttcc
     1801 tgtgccagga catccgcgag gcgatgccgc ccggcccgtt cgacctggtg ctgtgccgga
     1861 accttgccgt cacctatttc gacgaggcga cccggcgccg cgtgctcgcc gccatcgccg
     1921 cgtctatgcg cccgggcggc tcgctggtgg tcggcgccca cgagcagctg gcgcgcgccg
     1981 gccccgagtt cgttccggac cccggggatt ttccgatcta tcatcgcgtc tgaccggacc
     2041 tgagcgaggg cttgcggggg atcaaccatg gcgcggtcac catcgtccgg ccgaggcgcc
     2101 gaggccgaga cgcacgaacg gcgcgccatg atccgcgaga tcgaggccga ggcgcgcgag
     2161 accgcgcgct acaccgggcg cagcgcgttc gacgcacgga tcatggcggc actcgccgag
     2221 gtgccgcggc acgagttcgt gccggccggc gagcgcgact tcgcctattt caacgagccg
     2281 ctgccgatcg gccacggcca gaccatctcg cagccttaca tcgtggcgct gatgaccgac
     2341 ctgctggagc cgcagccgga gcatgtggtg ctcgaggtcg gcaccggctg cggctaccag
     2401 gcggcagtcc tgtcgcggct ggtgcgccgg gtctactcga tcgaggtggt ggccgcgctc
     2461 gccgcctcgg cgcgcgagcg gctggcccgg ctggggttcg acaatgtcga ggtccgcgcc
     2521 ggcgacggcg ccgccggctg gcccgagcac gcgccctatg acggcatcgt cgtcaccgcg
     2581 gcggcggccg agctgccgcc ggccctggtc caccagctca agcccggcgg gcggatggtg
     2641 atcccgctcg acagcggcct ctggcaggac ctcaagctgg tgatcaagga cgacaagggc
     2701 ggcatcgaca gccgtaccgt gctgccggtc gcgttcgtgc cgcttaccca cccaggcaaa
     2761 tagcgggcgg ggctcggtgg ccgccgatgc gtggctcgag atcgacggtg gccacggcga
     2821 gggcggcggc cagatcgtcc gctccgcgct cagcctggcc gcggtcaccg gccgggcggt
     2881 gcgcatcctg cgggtgcgcg ccggcaggcc gcggccgggg ctcgccgccc agcacgtcac
     2941 cgccgcgcgc gcggtggcgg cggtgtgcgc cgggcgcctg gacggcgacc ggctcggctc
     3001 gcaaagcctc gccctggagc cgaccgcgcc gccgcgggcg ggcgattacc tgttcgacgt
     3061 cgccgccgcg cgcacgggcg gcagcgccgg cgccgtcagc ctggtgctgc aagcgacgtt
     3121 gctggcgctg gcgttcgcga gcgggcgctc gaccttgcag ttgcacggcg gcacccacat
     3181 ggcgtggagc ccgagcttcg actattgccg cgacgtatgg ctgccggcgc ttgccgagat
     3241 gggcatcgcg ggcgaggccg agctgcgcgc gtggggctgg tttccggtcg gccgcggcga
     3301 ggtgcatgcc cggatcatgg gcgccgggcc cgggtttcgg ccgcggtcgg tggattggtc
     3361 ggcgcgcggc gggctggtcg cggtgcgcgg ccgcgcggtc gccgccaatc ttccggccca
     3421 tatcccgcag cggatgtgcg accgcgcgcg tgccctgctg gccgaggccg ggatcgagtc
     3481 ccggctcacg cccgtgcgcg tggtcgcggc gtgccccggc gccggcctgt tcctcaccgc
     3541 cgagtacgag cggggacgcg ccggcttcac cgcgctcggc gagcgcggca aggcgtcgga
     3601 gacggtggct gaggaggcgg tcgcggagct gctcgcccac cacgggtcgg gcgcggcgct
     3661 cgaccttcac ctggccgacc agatggtcct gcccgcggcg ctggcggacg gcgaaagccg
     3721 ctacacggcg gcccgggtca gcggccacct cgagaccatg gcatggctgg tcgagcgttt
     3781 cggcctggcc cgggtcgaga ttgcgccggc ggcgcgcgac gcccccgccc tgataacggt
     3841 caggccgcgg agtcagtcgt cgcaggagta gccgagctgg gccaggccct ctgccaggta
     3901 gtcgccgagc aaagggatgc cgagcagata ggtatcggta tggtcgctgc gcgcggcgcg
     3961 cgcctcgtgc agcgcatcct cccattcctc ggccgagaag tcgccgcgcc cgagccaggt
     4021 cagcgccacc aggttgacct gctcctcctc gttgagcgcg tcgatgaaac cgaccagctc
     4081 ctcgtagacg ggatcgttgg cgaagctctc gtgcacactg atcatgtcgg cctcggaaag
     4141 accggcggcg aagtcggggt ccgcaatctc gaccttggcg tcgaacgcgc gcacatgggt
     4201 gatcagcgcg cacaccttct cgatcgcgat gccgacgacc tgctcgtccc ggtcgccgtc
     4261 gtcgccgtat ccggggccgt aatcgtcgct catcctgcgg cctcctcagt caccgcgtgg
     4321 ccggcgcctc caaggtcccg ccgcggtcgt acatggtgag gtcccaccgt ccgtccattt
     4381 cgtcatggcg caagatgtac agcgcgccgt cgtcgccctt caccttgaaa taactgtgct
     4441 cgggcgcaag ccatcggtcg accacttccg acacctcgat cgcgcgcgcg cccatgtgca
     4501 ggcaccgcgg ggtctcctcg ccccggtgcc cggcataaca gtcgacacgt atctccatcg
     4561 ccccactccc gcatgccccc gctcaacttt gaccacccgg atcggctcgg tcaatcgcca
     4621 aggcgcggcc tcgggctacg gccggccccc ttgacccggg gcatcacgtg accgccttgt
     4681 tgggatcgcg tggcgcgcga acgaggcgtg gaatgcagcg gttccggtgc gatcgaatgc
     4741 tggcgcggct cgggcgcttc ctgcgggcgg cgggctacga taccgaaatc gccgacgacg
     4801 gcgcggacga tcgggacctg gtggcacggg ccgcgagcga agcgcgcgtt ctgctcacct
     4861 gcgaccgcgc gatcgcgcag catgcggcgg cgcgcggcgc ggtgatcctg ccggcgaacg
     4921 gcgtcgatgc ccaggcccgc gcactcgccg gcgcgctcga catcgactgg gagatggccc
     4981 cgttcacgcg ctgcctgatc gacaatgcgc gcctgcgcac ggcgcgcgcc gacgagatcg
     5041 cccgcctgcc ggcgcggtcg aggcagctcg gcgggccatt ccgggtttgt ccggcctgcg
     5101 ctcgcctgta ttggccggga agccatgtcc ggcggatgcg cgcgcgcctt gcccgctgga
     5161 gcaccgagat ccgcgacact tgacctgacg gcgcgtgcgc cccaaccttg gcctcatgtc
     5221 taccaccatc tcgcccgaca gcccgctcaa gatatcgatc tggattccgc tctgcctgac
     5281 tgcggcgggc ctcgtctatc tcgcgctcgc gatccttgcg gcaggcggcg cggcggtcca
     5341 ttagattgcg gcaaagtccg atatcgggac cgccgaccgt ccccggcggc ggcgcgcccg
     5401 ggttgctgcg gcgacggcgc cgatagtagc atgcgcgcca cctgggcggc ggcgccggcc
     5461 ggatcaatgg agggaatgac atgcggacgg tcgtcggaac cgttatcgga ttggtggtag
     5521 gcgcggtggt cggcgtgttc gtgctggcgc cgacatcggt cgagcgcggc gagccggcgc
     5581 ccggcgagca agcgggcttg acgaccgcgg cggcggctcc ggtccactgg aagatgccga
     5641 gctcgtttgc cggcgagatg gtccagctcg gcaccctggg caggcggttc gagacgcggc
     5701 tgatggaggc ctccggcggc accatggacg tcaagttcta cgagcccggc gcgctggtac
     5761 cggcgctcga gatcttcgat gcggtctcga ccggcgcggt cgacgccggc tggtcctcgc
     5821 ccggctactg ggcgggcaag attccggcgc ttcagttctt cgccgcggtc ccgtttggcc
     5881 ccgaccccgg cgagtacctg gcctggctct actacggcgg cggcaaggag ctgttcgagg
     5941 agatctacgc cccgcacaac atccacccgc tgcattgcgg catgatctcg cccgaaggct
     6001 cgggctggtt ccgcaacgag atcacctcgc tcgacgacct gaaggggctc aagatgcgct
     6061 tcttcgggct gggggcgaag gtgatggaga agctcggcgt ctccacccag ctgctcgccg
     6121 gcggcgacat cttccccgcc ctcgagctcg gcaccatcga tgccaccgag ttctcgatgc
     6181 cggcgatcga cctcaatctc ggcttctacc aggtcgccaa gcactactac ttccccggct
     6241 ggcaccagcc ggcgagcctg atcgagctga tcatcaacat ggaccggtgg aacgcgctca
     6301 gcgacgccca gcgggcgatg gtcgaggtca cctgcggcga cagcatccgc gagggcatcg
     6361 ccgagggcga ggcgatccag ttcaaggcgc tcgaggagtt gcagtccaag ggcgtcatcc
     6421 tgcatcgctg gtcgcccgag atcatggacg cgctgaggac ggcgtgggcc gaggtggtgg
     6481 ccgaggaatc ggcgaaggac gcggacttca agcgggtctg ggaatcgctt gacggcttcc
     6541 gccagggcta tgccctgtgg cggtcctacg gacgtctcga ctaggccgtc aaccgcgggc
     6601 ggacgccgag cccgggcgcc gacgtcgcgc gcgacttaca gggcccgttc cgggctcggt
     6661 accggtgccg tctgggtcgg ctgggcgcgc ggcgtcggac cggcgtccgc ccgcggcaag
     6721 ggcagctcga cgtgaaccgt ggtgccttcg ccgggattgc cgtcaatgcc gatctcgccg
     6781 cccatcagct cggccagccg gcggcagatc gacaaccgca gaccgccgtc ggcgtggttg
     6841 cggtcgcgct cggtcgacgg cccgtcctcg gtggttggct gagacgggcc ctggtcgacc
     6901 acctcgatgc gaatgcgttc gcgtccccga tcgtcgctac cggcgcgtgc ggcgtggatc
     6961 acgatgtcgc cggcatcggc gaagtcgagc gcgttgccca ccaggttgac gacgatctgg
     7021 cgcagacgca ccggatcggc cagcacccgc ggcgggatgt cgggggcgac gcgggtgacc
     7081 agctgcacgc ccttgcaccg ggcctcggcc gccagcatcg ccgccgatcc ctcgaccacc
     7141 gcctcgatcg aggtgggagc cggctcgagc tgcagctccc cggcctcgat cttcgacagg
     7201 tcgcggaatt ggttgatgac gccgagacac gcacgcccgg aatcgcggac gttgcccagc
     7261 atgcgccgct gctcgccgtc gaggtcggtc ttccacagca ggtcggccat gccgacgatc
     7321 ccggtcatgg gcctgcgcat ctcccggctc accgacgcga gcagctccgc tttcgccccg
     7381 cttcctgccg ccgcggcctg cttcgcctcg ctcagctccg cttccatgtg cttgcggtgc
     7441 gagatgtcgt cgtaccacga caggcgcgcg gcctcgcccg cctcggtggt cagcggcctt
     7501 gccgcgcgcc gcacccagag ctcggacccg tcggcccggt gcagccgcag atcggactcg
     7561 tcgaaactgc catcggcgcg ccacggcgga ggcgcggcgt ctcccgccgc cggctcgaaa
     7621 ccgtaccgcc tggcgtcgcc gcccgcgatc tcctctggcg cacggcccag catctcggcc
     7681 atgcggcgat tggcgaaggt gacccggccg tcggatgcgc gcacgatcag cgcaccgatc
     7741 gggctgccgt cgaggatcgc gcgcagccga tcgccactcc ggttcagccg tgtctcgctg
     7801 tcgctcaacc gctccttgac ttgactcagc tccgctcggg tctcgcgcat ccgttcctcg
     7861 tcctggctca cccgtgcccg ggcctggcgc agctggtcct cgctgcgggc caaccgctcc
     7921 ccgctctggc ggacctgcgc ttgcatctgg tgcagctgct gctcgctttg gctcagccgc
     7981 tctgcggtcc ggcttagctg cgcccgggtc tcgcgcagct gctcctcgct ccgactcgat
     8041 cgctgctcac cctggctcag ccgcgatcgg gtctcgcgca gatcctcctc gctttgagtc
     8101 aaccgctcga cgctctctct cagccgattt tcggtctcgt gcagtcgcgc ctcgcgctgg
     8161 ctcaaccgct ctgcgctctc gccgatctgc gcgtgggcct gctggagctg ctgcccgctc
     8221 tgagtcaacc gttcctcgcc ctcgtccagc cgggcctgag tgtcgcgcag ctgctcctcg
     8281 gcgatcttgc ggtcggtgac gtcggtgtac caaacgaagc gcgcgggctc gccgtcgtcg
     8341 gtctcgacct cagccatggc gctggcgacg acgcggcgct cgccgccgtc gctgctgcgc
     8401 aggcgcgcct ccaggcccgc gacctcgccg tcgcgatacc aggactcgac gaagtcctgc
     8461 caggcctccc gttcgaccca cggctcgtcg gcttggcgcc cgatgaggtg atcggcctcc
     8521 gcggccagaa gccgcgcaag cgccgcgttg acgtagatct ggctgccgtc ggacacgcgc
     8581 gcgatgccga tgccgaccgg cgccgcctcg agcacccggc gaagacgagt ctcgctgcgc
     8641 ctcaagcgct gctcatgccg cttgaggtcg gagatgtcgg cacacagcac gacggtgccg
     8701 acgatcttgc cgtcggcgtc cgcatagtcg ctggcgctgg ccaggcaggc cacggtgccg
     8761 ccgtccgggc gcaccagagt gacctcggag cgcgcgctcc ggccaacgat gtcccgtccc
     8821 gcctcgtcgg tgaacgagtc gggccgctgc cccagcagcg cctctcgggg tttgccgaga
     8881 atgcggcaga tggcgggatt ggcgtcgacg atcctgccgt ccggatcgat catccagacg
     8941 ccttcggcgg tggcgtcgag cacgctgcga atgcgggcgc cggcgagggc cgcctgccgc
     9001 tctgcctcct cgagcccggt cacgtcacgg acgaagcaca gccgcgcggg ctcgccgtcc
     9061 tgcgtttcgg tcggcaccac cgagaccagc accgacaacg gctcagcccc ggcggtctcg
     9121 agctgcgcct cgaggttgca ggcgctgccg cccgccggca ccgcgtcgag gatcgactgc
     9181 ggcccgggcc tgccgacgac acgctcgacc tcctcgccga ccatgccggc atacggcgcg
     9241 ttgcagctcg cgaccgtgcc gtcgcccgtg cgcaccacga ccgtgccgat cgggcatcct
     9301 tcgagcatgt gccgcaggcg ccgttcactg aacccgagcc gctcctcggc gcgcttgcga
     9361 tccgtgatgt cgtagtgcca caggatcaac gcctcccggc cctgatagaa gacacggtcg
     9421 tgatcgatca gggaccacca ggtggtggcg tcgaagcgcc gccgctcgac ctcgaccggg
     9481 gcttgccgct cttgggactc aaggcacgcg agcacccgct ggaggtccgc gcgctcgaca
     9541 aagctgtcgg cgatgtcctg cgacgccagc tgcgcctcgg actcggcgcc gaacagctgc
     9601 aggtagcggt cgttcacgta catgcgttga cgcgattgcc ggtcgatgat cgagatgccg
     9661 gcggggctgc tcgccaggat ctgatcgagg ctttcccggc ttcgcttcac ctccagctcg
     9721 gcgcgcttgc gctcggtgat gtcggtatgc caggacagca gggtgtcgac cccgtcttcc
     9781 tggtggcggt acaccgacat caggtcccag taccggctgc cgttgcagcg ccggcggagc
     9841 acctcgatat cgcgaaccgc gccttcgcgc tccagctggg cgatgatgtc ggcgcgctgg
     9901 tcggggtcgg cgtaacgctc gatcgccgtg ccttcgatga cgccgtcggt gtcctcgtag
     9961 ccgttgtcct cgacgtagcg ccggttggcg aagatgtagc tgccgtcgtc gatgcgcgtg
    10021 atggcgacac cgaccgggca atgctcaagg atcgcgcgca gccgccgctc gccgtcgccc
    10081 gcctgctgct cggcgcgcct gagaacggtg acgtccgaac aggtggtcag atggccgata
    10141 ccagggattc gcctgcttcg gatctcgacc acggtgccgt cggaccgcac gcgctccgcc
    10201 tgttgcggct cgaacgactt tgccagcccg atccgctcgc gcaccatccg ctcgacgtcg
    10261 ccggggccgt agtcgccgcg ctcggcactg aagcggatga atgcctcaag cgaatcgccg
    10321 atcgcgaagc ggtctttcgg gacctcggcg aggtcgagga acttgtcgtt gaacgccacc
    10381 gcctccagcg cttcgtcgca cagcgacacg ccctggtcga cgccggcgag cacggcgtcg
    10441 aggacccgcg tccgctcggc cagcttcgcc tcggcggtct tgcggtcggt gacgtcgagc
    10501 aggataccgt cgagatatgc cggcgcgtcg ccctcggccg ggctcaccca gccttgctcg
    10561 gcgatccaac gcacctcgcc ttcgccgcgc acgatccggt actcgacgtc gtagcgggcg
    10621 ccggcctgag ccgcttgcac aagcacgtcg cggacctttt gccggtcctc cgcgtggatc
    10681 atttgggcga agccgctcgc cggtcggccg atgaagtcgc cggccgggcg cccgctcagc
    10741 cgctcgacgg cgtcgctgac gaagtccacg gtccactggc cgtcgagcgc cagccgatag
    10801 accgcgccgg gcaggttggc gatcagggtg ccgttctgct cctcggccct cgccaggccc
    10861 tccaagcatc tcttgtgctc cgtgacatcg gcgagaacga ccactagccc gccgtccctg
    10921 agcggcatct tgcgcgcgct gaggattcgc ccctcgctgg cgcgaagctc gctctcggcc
    10981 ggttcctggc tggcgaagtc ggcgaggcat tgctcgacca gctcgtcctc gtcgcccggc
    11041 ccgaacagcc ccgcgcgcgc cgcctcgacc atcaccgggc ggaccggctc gcccagcccg
    11101 agcaggtcgg gcttgacgcc gagcaaggtc gcgcagcgcc ggttgaacag gcggaagcgc
    11161 aaattccggt cgagcacgaa gatgccgtcg ggcaggttgt ccaccgccgc gtgaatcacc
    11221 gtgtcctggt cggcaagcgt cttgcgcacg tggtcgagct cgcgcaggcg ttgggtgaag
    11281 ctcgagtcct tctggtcggc ggcatcgacg acggaggcga agctgcgcgc catgtcggcg
    11341 atctcgtcgc gcccgtcgac ctcgatcccc tcccgggcgc cggaatcgtg ggcggtcatc
    11401 gcgcggtgca gccgcttgat tcgcgtgacc acacgccggt cgacgaagcc ggcgagggcg
    11461 acggcgccga gaacgccgat cagcgacatc ccgatcagaa ggtatgacgg caaagcgatg
    11521 gcaccctcgg cgtccgccga ggcggcgagg gccgcgctcg acagccggtc ggcgatcccg
    11581 gcggcggttg cggccagccg gtccatcacc gccatgcgtc gcaaggcgcc ctggaaccgc
    11641 aaatcgagca gcagcctctc gatccgctgg tcgaagacgt cggcggcggt cttgatctcg
    11701 accagcagca tctcggccag cagccgcccg ggcgcgacgc tgccctcgtc ctgcagcccg
    11761 tcgaccttct ggcgcgcctg ctcgaactgc cggtcgaagc tcgccttgag ctcgtccacc
    11821 tccttcgggt cgtcactggc gatggccgcg agctgagccc ccgccgcccg catcatcggc
    11881 accgccgccg cctggcgccg gtcatccgag gcctcgccgc tccaggcgaa cttctcgatt
    11941 cgccgctgga gcgccgccag cctggccgcc cgctcggcga gagcgtcgtc gagcgcgatt
    12001 cgtcgttcga ccagcgactc cagtctctcg agcacgccgg tcagcagctg catcgacgcg
    12061 ttcagctcct cagcctcgcc ggtcgaggcc acggactcgt ccagcttgtc gatgctctcg
    12121 gccagctgcc cggcgtcggc gcgcaagccg agaatgccgc tctgtaccgc gaacgccccg
    12181 tcgaggttgg cgaggttgcg ggcgctgcgg tcgaccttgt cggccgcccg cctcagctcg
    12241 ttcgcctgag tcagcgccgg cacggcggtg cccgcgacgg aaccgacatt gatcgagaac
    12301 atgacgaacg ccgacaacgc gaccacggcg accagcaccg tgagcccggc aattccggcg
    12361 acaccggccc gaagatacag gccgatgctg cgagcttccc agtcgcctct ggcgatgccc
    12421 accaaacttc gcactgctgc aactcccaga cttgccccgg tgcggggccg atcaatcggt
    12481 gccgggcaac ggtccgaaat cgaaatccgg gcacccgccc cgtgatgaat atgcggcgac
    12541 ctcgatcttt cccctgatcg cctcgaaggt cagagtctcg atccgcctct gcatgtcgtc
    12601 gtcgatcagc aagacgttgt gcctgtcgag cgcccaatcg acgccgccgt cgcgcagccc
    12661 cagccggcgt gtgcccggct gccacgcgcc gtcggcgatg tcgctcaacg ccgcgaacac
    12721 cgcgacatcg aggcgcttga tcatggaggt gagcacatgc cccggggcga ggccgttctg
    12781 gttgccgccg gcgccgatcg cgagaatgcc ctgccgggcg gcggccgcga tgatgccgtc
    12841 accgctcgcg ccggcggcgt gatagacgac gtcgacaccg ccctcggcga gagtgcggac
    12901 aagctccgtg cccttgcccg catcggccaa cgccgggtcg gtgtcgccgg ccatcgccga
    12961 gaccacctcg atgcggggat tgagacggcg cgcgccctcg aagaaggcgc aggcgaaggt
    13021 gcgggcgagc gcgccgtccc ggccgccgac aaagccgacc ttgccggtct tcgacgccat
    13081 cgcggcgagg gcgccgacca ggaacgagcc ctgctccacg ttgaactcga tggattcgac
    13141 gttgccgaga tcaacgaccg cgtcgaccag gacgaagcgc acggcctggt gctccggcgc
    13201 gagctggcgg aaggactcgg caaacacggg accgacaccg acgacgatgt cgtgcccggc
    13261 ctcgatcgcg ccggtcacca ggtcgcgggc ggccgaggcg tcgatgtcct cacccgccgg
    13321 cgggccgagg atcgccacct cgatgccgcg catgcgcccg aacgagcggg cgccgtcgag
    13381 cgccaggcgg gcgctgccgt cgccctcgac atcagcggtg gcgaacagca ccgccgggct
    13441 gagcgcctgc gcggagtaag gggtcatcag cgcgcacagc aggaccagga aacgacatcg
    13501 ggccaaggct gcacgcagcc tgtcccgcga gggcacgccg tctgcggtca tcgtcatcga
    13561 gaggccttcg ctaccaaccg caataggatt tattacgatg gcggcgagac gcacctttta
    13621 gcgcggcgaa gaacgcgccg aggtgacgat tgtcaccggc ggtcccgatc gccgggtcgg
    13681 tcatccccgc ggccggtagg ggggcacccc ggggtccggc acgtacagtc ccgcaggcgc
    13741 tggcccggtc tgccagacca cgtcgatcgg gatgccgccg cgaggattcc agaagcccgc
    13801 gatcctgagc cagcgcggcg cgaccgccac gacgatccgc ttgccgatcg cgaccgtgca
    13861 atcctcgtgg aacgcggcat ggttgcggaa cgcgtgcagg tagagcttga gcgacttcga
    13921 ttccaggatc cagcggtcgg ggacatagtc gatcacgaag gtcgcaaagt cgggctggcc
    13981 ggtgaccggg cacagcgagg tgaactcggg gcaggtgaag cgggccaggt agtcgacatc
    14041 gacgtggtcg ttcgccacct tttcgagctc cgcctgctct ggcgaggccg ggattgcgcg
    14101 cgcccgcccg agctgggtca gtcggtcaaa tctctcggtc accgggtttc ctttccgctc
    14161 ggggctcgga tcctcgacga tgccgcctgc gcccgccgcc ggcaaggcct tcgagcgcgc
    14221 gccatggacg caccgcgtcg gcgccgctag catgcaccga agcctgcggc ggcgcctcgg
    14281 ggggacctgg tgatggatgt cggcctgttc tgcctgctca atcagcgcga caagagccag
    14341 gcgccgcgcc aagtcatcgc cgagacggtc gagcaggtcc gcctcgccga ccggatcggc
    14401 ctgtcgatcg cctggttcgc cgagcaccac ttctccaact actgcctgtg cgcgtcgccc
    14461 ctgctgatga tcggccactg cgccgcagtg acgtcgcgca tccggctcgg caccggcgtg
    14521 ttggtgctgc cgctttatgc gccgatgcgc ctgctcgccg agatcggctt cgccgattgc
    14581 ctgagcggag gccggctggt gctcggtatc ggcagcggct accaggacta cgagttcgag
    14641 cgcttcggca ccgacctcgc cgagtcgaag gagcgcacgc tcgagctgct cgacatgatc
    14701 gagcggagcc tggcgacggg gacggtcgac catgacgggc gctactacac gcagccggtc
    14761 acccggctca gcgtcacgcc gctgcagcgc ccccaccccg acatctggat cgccggcgcg
    14821 gcgcccgcgc tgatgcagcg cgcggcgcgg tccggctatc cggtgatcgt cacccagcgc
    14881 ctgtccgggg tcgatttcct ggtgtcgggg cgcgagtggt gcgcgcgcgc ctatcgtgac
    14941 gccggggttg atccggcgcg catgca
//
LOCUS       DASWHU010000278         9929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3463878, whole genome shotgun sequence.
ACCESSION   DASWHU010000278 DASWHU010000000
VERSION     DASWHU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3463878"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGB88_10235"
     CDS             <1..217
                     /locus_tag="VGB88_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9647868.1"
                     /translation="SYTRCETLARQLLIYGRAKTIEEMVAEIDAVGPEHVRACMTRLL
                     GTASPTVTALGPVGTLPDYPAVAGRLH"
     gene            226..480
                     /locus_tag="VGB88_10240"
     CDS             226..480
                     /locus_tag="VGB88_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX9647869.1"
                     /translation="MAAPGAGGGQQVTILDVRGLACPLPVLKARRAIRDVAAGGLLKV
                     LATDPASVADFKSFCAVTGHRLEDSAEAGGVYSFVIRAKE"
     gene            518..1186
                     /locus_tag="VGB88_10245"
     CDS             518..1186
                     /locus_tag="VGB88_10245"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9647870.1"
                     /translation="MRGRPIDAGRVFLRPPARGDWRSWAALRAESRAFLEPWEPAWSR
                     DALTRKAFMRRLRAYGMNSQSDAGYSFFVFRRDDNALLGGVSLSHVRRGISQTASIGY
                     WIGAPFAGRGYMTEALSGLLPFAFDRLCLHRVGASCMPHNLASRAVLKKVGFSEEGFA
                     RKYLRINGIWQDHVLYAMLDSDLRPALMHSVAAKEMPVRRFAAQVDRALAPARRAKLG
                     PERG"
     gene            1285..1743
                     /locus_tag="VGB88_10250"
     CDS             1285..1743
                     /locus_tag="VGB88_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9647871.1"
                     /translation="MASRDQGASDEARALGTYVKLVRASQTLLAAVARRVAAAGLTQS
                     QFGVLETLYHLGPMSQRALGRKLLISGGNVTMVVGNLERRGLIARRRRAEDARVMDVS
                     LTPKGRRLVARLFPQVARLVVAKMAALGGREQAQLGRLARKLGRAAAELD"
     gene            1911..3779
                     /gene="cobT"
                     /locus_tag="VGB88_10255"
     CDS             1911..3779
                     /gene="cobT"
                     /locus_tag="VGB88_10255"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX9647872.1"
                     /translation="MSERSEPASETFRRAVASATRAIARDGELEVSFNAAGPRLEGEK
                     ALIRPPSRELKPDEVGEIRGEADALALKRRYHDDRVHGARRPPGQIAQSVYDALEQAR
                     CEALGARTMAGVAGNLAAALKARCREQRFDQVQDKADAPIVEIINLLARERLTGARPP
                     EGARTMCDLWRPWLEQRIGRDFDALAGEINDQAAYAEDLRKLIAHLNFADEDENEASS
                     TETDPGVDDAEQENLDSQSDGESTDQPVELSGSAGEDAEEASAEDSMEIELDARDDPG
                     AAGDEEAPAEGQPWRPPMDRSNVRRSGYRVYTLDYDEVVEAAELCEPEELTRLRSHLD
                     QQLAALHGVITRLANRLQRKLLAKQTRSWEFDLDEGILDTARLARVITSPSYPLSYKH
                     EKETTFRDTVVTLLLDNSGSMRGRPISVAAMSADILARTLERCGVKVEILGFTTRAWK
                     GGRARERWLADGKPPNPGRLNELRHIVYKSADAPWRRARKGLGLMLREGLLKENIDGE
                     ALLWAHQRLLARPEERRILMVISDGAPVDDSTLSVNSGNYLDRHLREVIRWIEAASPV
                     ELVAIGIGHDVTRYYRRAVTIVDAEQLGGTMTEKLAELFDEEVGAFQPAAISPAIV"
     gene            3818..4744
                     /locus_tag="VGB88_10260"
     CDS             3818..4744
                     /locus_tag="VGB88_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX9647873.1"
                     /translation="MALVLAAPAQAREPIAIDARAVPLDAGDGGRARVGALAWRGGLE
                     LRSADPRFGGLSGLVVAADGAAMIAITDAGNWVGARLDYDRRGRLAGLSDAWIAPLKD
                     QAGVPLAGKTEGDAEDVTALPDGGYLVAFEQRHRLWRYADVEAAAAAYPAPDLGALPA
                     NGGIEALGALADGRVLALTEAYFIVPGGLAGWLIGGGQALTVIYPGDGYFEPTALSVL
                     DDGRVLVLERGYSVALGVKGRLILLDQAFGPGARIAPQEIARFEPPLQVDNFEGLAVR
                     RGGDGALLVYIASDDNFSPLQRTLLLMFALVE"
     gene            complement(4748..5278)
                     /locus_tag="VGB88_10265"
     CDS             complement(4748..5278)
                     /locus_tag="VGB88_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEX9647874.1"
                     /translation="MTILRQLWAPVAAMVAVVVASNIAVRFPINDWLTWGAFTYPLSF
                     LVTDLSNRAFGPARARQVVLVGFVLGVALSLLVPAGTRIAVASGSAFLAAQLLDVLIF
                     DRMRRLAWWQAPLASSLVASALDTALFFGLAFAGTGLPWVTWGVGDYAVKLAMAATLL
                     LPYRAVIRLLPVWRPA"
     gene            complement(5308..5598)
                     /gene="rpmB"
                     /locus_tag="VGB88_10270"
     CDS             complement(5308..5598)
                     /gene="rpmB"
                     /locus_tag="VGB88_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9647875.1"
                     /translation="MSRRCQLTGDGALRGMHVSHAHRRTPRHFQPSLQMYSLFSDALG
                     RVVRLKLATHTLRSVEHRGGLDPYLTSARARELTPFGRRLKSKVEKAAAKAD"
     gene            5758..7410
                     /locus_tag="VGB88_10275"
     CDS             5758..7410
                     /locus_tag="VGB88_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9647876.1"
                     /translation="MSDRLRLYSGLVLLVYVVGHLSNHALGIVSLATMEAGLPVTIEP
                     WRTGPGTVLLGGAALVHMATALKSVFVRRSLRLPGWQYAQIGLGLLIPLLLAGHLIGT
                     RGLVELFDVHGSYAFELAVLWVVAPQYGVLQVVVLVVVWSHACIGLDAWLRLKPWYAR
                     TRGLALALAVMVPTLALAGYVSAGMEVTALAADPAWFNGVFAESKAPPDALPFVETWM
                     VRTQLGFFALFAGLALVQAGRGLVGRRARRAKITYVGRRTIDVLPGATVLDCLRAAGI
                     PHASVCGGRSRCSTCRIHIDDGADSLPAPDGEELRVLRRISAPASVRLACQLRPHADL
                     AITPLLPPTATAADAYLRASHRASEERTIAVLFADLRGFTRIAEARLPYDVVYVLNRY
                     RAAMADAIERAGGVVNEFVGDGIMALFGLDGEAGAACRQALAAARLMAERLDQLNVTL
                     AAELDDPLRIGIGIHVGPVIVGEMGYANLKAITAVGDVVNTASRLEEMTKSHAAQLVV
                     SDEVARLAGESLALAATHQVAVRGRAEPMTIRVVADAGALTP"
     gene            complement(7400..9838)
                     /locus_tag="VGB88_10280"
     CDS             complement(7400..9838)
                     /locus_tag="VGB88_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9647877.1"
                     /translation="MAERARVVVIGAGIVGSALAYHLARRGRDGIVVLDRGPLFDTGG
                     STGHAPGLVFQTSSSRLLTRLARMSVRLYRALRLDGAPGWRAVGGIEVATTDARLADL
                     HRKLGSARSWGVAEAALITPAEVAARLPLIDRGRILGGLWVPGDGVADGPRLCRALAR
                     RAEATGAARFQGETEVTGFEVSAGRLRAVLTDCGAIETEVAVICAGLWSPGIGKLAGI
                     SVPLVAVEHQLARTTALPALAGTGEDEIAHPILRHQDQDLYVRQVGDRYVIGSYRHAP
                     RLVEADDLGATALRPFTAADFAPAFAEAKALLPLVADARIDHAINGMFSFTPDGLPLL
                     GPTEVDGLWLGAAVWVTHAGGAARALADWLVDGEPRIDLHAADANRFHAHQRTPAYVC
                     ARGAQQYVEVYDVIHPRQQIAAPRGLRLSPFQARLEALHPEMVEVAGWERPLCFAANE
                     GLLDRFAVPGRNGWAARHWSPVEGAEHLATRAGVALFDLSPFVKIELSGPGAARLLQW
                     LCASQIDRPPGAVVYTAMLGWRGGIMADLTVTRLAADRFLIVTGAATGMHDLAWIRGH
                     AAGDDTVRIADVTSGLAALGLWGPKARAVLAAACPDDDMADAACPYLAARRLHVGAVP
                     VRALRISYAGELGWELYAPTECALALWDALWQAGAAHGLVAAGIGALDSLRIEAGRRL
                     WGADIGTDDDPYQAGLGFAVRLDKGDFLGRAALMRIRERRIARRLCCLALDDAHAAVM
                     GGEPILDRGRPVGFVTSANFGYSTGRFIAYGWLPARLAAPGTPVTVEYFGAALVATVA
                     ADPVFDPARARVRG"
BASE COUNT         1380 a         3567 c         3692 g         1290 t
ORIGIN      
        1 cagctacacg cgctgcgaga cgctggcccg ccagctgctg atctacggcc gggcgaagac
       61 gatcgaggag atggtggccg agatcgacgc ggtcgggcct gagcacgtgc gcgcgtgcat
      121 gacgcgcctg ctcggcaccg cttcgccgac ggtgacggcg ctcggcccgg tcggcacctt
      181 gcccgactac ccggcggtcg ccggccggct gcactgagca tggcgatggc ggcgccgggc
      241 gcgggcggcg gccagcaggt cacgatcctg gacgtgcgcg gcctcgcctg tccgctgccg
      301 gtgctcaagg cacgccgggc gatccgcgac gtcgccgccg gcggcctcct caaggtgctc
      361 gccaccgatc ccgcctcggt cgccgatttc aagagcttct gcgcggtcac cggccaccgg
      421 ctcgaggaca gcgccgaggc cggcggggtc tacagcttcg tcatccgggc caaggagtga
      481 gcgcgtgatc gcgctgttga agacgctgtc cgagctgatg cggggccgcc cgatcgatgc
      541 cgggcgcgtc ttcctgcgcc cgccggcgcg cggcgactgg cggagctggg cggcgctgag
      601 ggcggaaagc cgtgccttcc tcgagccgtg ggagccggcg tggtcgcgcg acgcgctgac
      661 ccgcaaggcg ttcatgcgcc gcctgcgcgc ctacggcatg aacagccagt ccgatgccgg
      721 ctactcgttc ttcgtctttc gccgcgacga caacgcgctg ctcggcggcg tgtcgctgag
      781 ccacgtccgg cgcggcatca gccagaccgc cagcatcggt tactggatcg gcgcgccgtt
      841 cgccggccgc ggctatatga ccgaggccct gtcgggcctg ctgccgttcg ccttcgaccg
      901 gctgtgcctg caccgggtcg gcgcctcgtg catgccgcac aacctggcca gccgcgcggt
      961 gctgaagaag gtcggcttca gcgaggaggg gttcgcccgc aaatatttgc ggatcaatgg
     1021 catctggcag gatcacgtgc tttatgccat gctggacagc gatctgcgcc cggccttgat
     1081 gcacagtgtc gccgccaagg aaatgccggt gcgccggttt gctgcgcagg tggaccgcgc
     1141 cctggcgccg gccaggcgcg ccaagctggg gcccgagcgg ggctgacctg gcggacttgc
     1201 cgcccgatgg ggcgggactt ccagagggcg cggagccgaa tccggccgcg ggggccggtg
     1261 ccgctagccc gaaggagagg cgagttggca tcgcgcgacc agggcgcttc cgacgaggcg
     1321 cgggcgctcg gcacctacgt caagctggtg cgggcgtcgc aaacgttgct cgccgcggtg
     1381 gcccgtcggg ttgccgcggc ggggctcacc caatcgcagt tcggcgtcct cgagacgctc
     1441 taccacctcg gtccgatgag ccagcgcgcg ctcgggcgca agctgctgat ctcgggcggc
     1501 aacgtgacca tggtggtggg caatctggaa cggcgcgggc tgatcgcgcg ccgccgccgg
     1561 gcggaggacg cgcgggtcat ggacgtgtcg ctgacgccca agggccggcg cctggtcgcg
     1621 cgcctgtttc cccaggtcgc gcgcctggtg gtggcgaaga tggcggcgct gggcgggcgc
     1681 gagcaggcgc agctcggcag gctggcgcgc aagctggggc gggcggccgc cgagctcgac
     1741 tgagaggctg cgaatgaatc ggctgtcgcc cttcgagacg ctcgctccgc tcgctcctca
     1801 ggacgagggg gttttgaaaa tcaatcattt acctcaccca gaggagcacc cgacgggcgc
     1861 gtctcgaagg gcgaggcaaa agattttcgg gcactgagcg gggccgagcg gtgagcgagc
     1921 ggtcggagcc ggcgtccgag accttcaggc gagcggtcgc cagcgccacc cgcgcgatcg
     1981 cccgcgacgg cgagctcgag gtgtcgttca acgccgccgg ccccaggctc gagggcgaga
     2041 aggcgctgat caggccgccg tcgcgcgagc tcaagcccga cgaggtcggc gagatccgcg
     2101 gcgaggcgga cgcgctggcg ctcaagcggc gctaccacga cgacagggtc cacggcgccc
     2161 gccgcccgcc cggccagatc gcgcagtcgg tctatgacgc gctcgagcag gcgcgctgcg
     2221 aggcgctggg tgcgcggacc atggccgggg tggccggcaa cctggcggcg gcgctgaagg
     2281 cgcgctgccg cgagcagcgc ttcgaccagg tccaggacaa ggccgatgcg ccgatcgtcg
     2341 agatcatcaa cctgctcgcc cgcgagcgcc tgaccggcgc ccgcccgccg gagggcgcgc
     2401 gcaccatgtg cgatctgtgg cggccttggc tcgagcagcg gatcggccgc gacttcgacg
     2461 cgctggccgg cgagatcaac gaccaggcgg cctatgccga ggacctgcgc aagctgatcg
     2521 cccatctcaa cttcgccgac gaggacgaga acgaggcgtc cagcaccgag accgaccccg
     2581 gcgtcgacga cgccgagcag gaaaacctcg actcgcaatc cgacggcgaa agcaccgacc
     2641 agccggtcga gctttccggc agcgccggcg aggacgccga ggaggcgagc gccgaggaca
     2701 gcatggagat cgagcttgac gcccgtgacg accccggtgc cgcgggcgac gaggaggcgc
     2761 cggccgaggg ccagccctgg cggccgccga tggaccgctc gaacgtccgc cgcagcggct
     2821 atcgcgtcta caccctcgac tacgacgagg tggtcgaggc ggccgagctg tgcgagcccg
     2881 aggagcttac ccggctgcgc agccatctcg accagcagct cgccgccctg cacggcgtca
     2941 tcacccggct ggccaaccgc ttgcagcgca agctgttggc caagcagacg cggtcgtggg
     3001 agttcgacct cgacgagggc atcctcgata ccgcgcgcct ggcgcgggtg atcaccagcc
     3061 cgagctaccc gctgagctac aagcacgaga aggagaccac gttccgcgac accgtggtca
     3121 cgctgttgct cgacaactcg ggctcgatgc gcgggcggcc gatctcggtg gcggcgatga
     3181 gcgccgacat cctggcacgc acgctcgagc gctgcggggt caaggtcgag atcctgggct
     3241 tcaccacgcg cgcctggaag ggcgggcggg cgcgcgagcg ctggctggcc gacggcaagc
     3301 cgcccaaccc ggggcggctg aacgagcttc gccacatcgt ctacaagtct gccgatgcgc
     3361 cctggcggcg ggcgcgcaag ggcctcgggc tgatgctgcg cgaggggctg ctgaaggaga
     3421 acatcgacgg cgaggcgctg ctgtgggcgc atcagcgcct gctggcgcgg cccgaggagc
     3481 ggcgcatcct gatggtgatc tcggacggcg cgccggtcga cgactcgacc ttgtcggtca
     3541 actcgggcaa ctatctcgac cgccacctga gggaggtgat ccgttggatc gaggccgcct
     3601 cgccggtcga gctggtggcg atcggcatcg gccacgacgt cacccgctac tatcgccgcg
     3661 cggtgaccat cgtcgacgca gagcagctcg gcggcaccat gaccgagaag ctcgccgagc
     3721 tgttcgacga ggaggtcggc gcattccagc cggcggcgat ctcgcccgcg atcgtttgac
     3781 aatcccggcc gcggtgcggg tcgccgcgtg cgccctggtg gcgctcgtgc ttgcggcgcc
     3841 ggcgcaggcg cgcgagccga tcgccatcga cgcccgggcg gtgccgctcg atgccggcga
     3901 cggcgggcgc gcgcgggtcg gcgcgctcgc ctggcgcggc gggctcgagc tccgctccgc
     3961 cgacccccgc ttcggcggcc tgtccgggct ggtcgtcgcc gccgacggcg ccgcgatgat
     4021 cgcgatcacc gacgccggca actgggtcgg cgcccgcctc gactacgacc ggcgcggccg
     4081 gctcgcaggc ctgagcgacg cctggatcgc gccgctcaag gaccaggccg gcgtcccgct
     4141 ggcgggcaag accgaaggcg acgccgagga cgtcacggcc ttgccggacg gcggctatct
     4201 ggtcgccttc gagcagcgtc accgcctgtg gcgctatgcc gacgtggagg cggccgcggc
     4261 ggcgtacccg gcgcccgacc tcggcgcgct gcccgccaac ggcggcatcg aggcgctcgg
     4321 cgcgctcgcc gacgggcggg tgctggcgct taccgaggcg tacttcatcg tccccggggg
     4381 gctggccggc tggctgatcg gcggcggcca ggcgctgacg gtgatctatc cgggcgacgg
     4441 ctacttcgag ccgaccgcgc tttcggtgct cgacgacggc cgcgtgctgg tgctcgagcg
     4501 cggctacagc gtggcgctcg gggtcaaggg ccgcctgatc ctgctcgacc aggcgttcgg
     4561 ccccggcgcc cggatcgcgc cgcaggagat cgcccgcttc gagccgccgc tgcaagtcga
     4621 caatttcgag ggcctggcgg tgcgccgcgg cggcgacggc gcgctcctgg tctacatcgc
     4681 gagcgacgac aacttcagcc cgctccagcg caccttgctg ctgatgttcg cgctggtcga
     4741 gtagcgatca ggccgggcgc cacaccggca gcagccggat caccgcgcga taaggcagca
     4801 gcagggtcgc cgccatggcg agcttgaccg catagtcgcc caccccccag gtcacccagg
     4861 gcaggccggt gccggcgaag gcgaggccga agaacagcgc ggtgtcgagg gcggacgcga
     4921 ccagcgacga ggcgagcggc gcctgccacc aggcgagccg gcgcatgcgg tcgaagatca
     4981 gcacgtcgag cagctgggcg gcgaggaacg cgctgcccga ggcgaccgcg atccgcgtcc
     5041 ccgccggcac caacagcgac agggcgacgc cgaggacgaa gccgaccagc accacctggc
     5101 gcgcgcgcgc cggcccgaag gcgcggttcg acaggtcggt gaccaggaac gacagcggat
     5161 aggtgaacgc gccccaggtc agccagtcgt tgatcgggaa ccggacggcg atgttcgagg
     5221 ccaccaccac cgccaccatg gcggcgaccg gcgcccacaa ctgccgcaaa atcgtcatcg
     5281 cccgtggctg gcgcgcggcc ggcggcgtca gtcggccttg gccgcggcct tctcgacctt
     5341 gctcttcagc cggcggccga acggcgtcag ctcgcgcgcg cgggcgctcg tcaggtacgg
     5401 atcgagaccg ccgcggtgct cgaccgagcg caaggtgtgg gtggcgagct tgaggcggac
     5461 cacgcgcccc agcgcgtcgc tgaacagcga gtacatctgc aggctgggct ggaagtggcg
     5521 cggcgtgcgc cggtgggcgt ggctgacatg catgccgcgc agcgcgccgt ccccggtcag
     5581 ttgacaccgt cgcgacatcc gtccgtcctt cgctcaagtc ctcgaagccg cgccccccaa
     5641 ggcgccggaa agcgccgttt ctatgccagc ccggccgcag gcgtcaaggc cggcgccgat
     5701 gttgccgggc tgcggcgttg gcggcacact cgccgcgcgc tcaggcatag ggactccatg
     5761 tcggaccggc tcaggctcta cagcggcttg gtgttgctcg tctacgtcgt cgggcacctg
     5821 tccaaccacg cgcttggcat cgtctcgctg gcgaccatgg aggcgggctt gccggtgacc
     5881 atcgagccgt ggcgcaccgg ccccggcacc gtgctgctcg gcggcgcggc gctggtccac
     5941 atggccaccg cgctcaagtc ggtgttcgtc cgccgcagcc tgcgcctgcc cggctggcag
     6001 tacgcccaga tcgggctcgg gctgctgatc ccgctgctgc tcgccggcca cctgatcggc
     6061 acccgcggcc tggtcgagct gttcgacgtc cacggcagct atgccttcga gctggcggtg
     6121 ctgtgggtgg tggcgcccca atacggggtg ctccaggtgg tggtgctggt ggtggtgtgg
     6181 agccacgcct gcatcgggct cgacgcctgg ctcaggctca agccgtggta cgcgcgcacg
     6241 cggggtctcg cgctggcgct ggcggtgatg gtgccgacgc tggcgctcgc gggctacgtc
     6301 tccgccggca tggaggtgac cgcgctcgcc gccgaccccg cctggttcaa cggcgtgttc
     6361 gccgagtcga aggcgccgcc cgacgcgctc cccttcgtcg agacatggat ggtgcgcacg
     6421 cagcttggct tcttcgccct gttcgccggg ctggcgctgg tccaggccgg gcgcgggctg
     6481 gtcgggcggc gggcgaggcg cgccaagatc acctatgtcg gccggcgcac gatcgatgtc
     6541 ctgccgggtg cgaccgtgct cgattgcctg cgcgcggccg gcatcccgca cgcctcggtg
     6601 tgcggcggcc gcagccgctg ctcgacctgc cgcatccaca tcgacgacgg cgccgactct
     6661 ctgccggcgc ccgacggcga ggagctgcgc gtgctcaggc gcatctcggc gccggcctcg
     6721 gtgcggctcg cctgccagct gcgcccgcac gccgacctcg cgatcacgcc gctcttgccg
     6781 ccgaccgcga ccgcggccga cgcgtacttg cgcgccagcc atcgggcgag cgaggagcgg
     6841 accatcgcgg tgctgttcgc tgacctgcgc ggcttcaccc ggatcgccga ggcgcgcctg
     6901 ccctatgacg tggtctatgt gctcaaccgc tatcgggcgg cgatggcgga cgcgatcgag
     6961 cgcgccggcg gggtggtcaa cgagttcgtc ggcgacggca tcatggcgct gttcgggctc
     7021 gacggcgagg ccggggcggc gtgccgccag gcgctcgccg cggcgcgcct gatggccgaa
     7081 cgcctcgacc agctcaacgt cacgcttgcc gccgagctcg acgacccgct gcgcatcggc
     7141 atcggcatcc atgtcgggcc ggtgatcgtc ggcgagatgg gctacgccaa cctcaaggcg
     7201 atcaccgcgg tcggcgacgt ggtcaacacc gcgagccggc tcgaggagat gaccaagagc
     7261 cacgccgcgc agctggtggt ctccgacgag gtcgcgaggc tcgcgggcga atcgcttgcg
     7321 ctcgcggcga cgcaccaggt cgccgtgcgc gggcgcgccg agccgatgac aatccgggtg
     7381 gtcgccgacg ccggcgcgct caccccctga ctcgcgcgcg ggcagggtcg aacacggggt
     7441 cagccgccac cgtcgccacc agggcggccc cgaaatactc gaccgtgacc ggagtccccg
     7501 gcgcggcgag gcgcgcgggc agccagccgt aggcgatgaa ccggccggtg ctgtagccga
     7561 agttcgcgct ggtgacgaag ccgaccgggc ggccgcggtc gaggatcggc tcgccgccca
     7621 tgaccgcggc gtgcgcatcg tcgagggcga ggcagcacag cctgcgcgcg atccggcgct
     7681 cgcggatgcg catgagcgcg gcgcggccga ggaagtcgcc cttgtccagg cgcaccgcga
     7741 agccgagccc ggcctggtag gggtcgtcgt cggtgccgat gtcggcgccc cacaggcgcc
     7801 ggccggcctc gatcctgagc gagtcgagcg cgccgatgcc ggcggcgacc aggccgtggg
     7861 cggcgcccgc ttgccacagc gcgtcccaca gcgcgagcgc gcactcggtg ggggcataga
     7921 gctcccagcc gagctcgccg gcgtaagata tgcgcagcgc gcgcaccggc accgcgccga
     7981 catgcagccg gcgcgccgcg agatagggac aggcggcatc cgccatgtcg tcgtccgggc
     8041 acgccgcggc gaggacggcg cgcgccttcg gcccccacag gccgagcgcg gcgaggcccg
     8101 aggtcacgtc cgcgatccgc accgtgtcgt cgccggcagc gtggccgcga atccaggcga
     8161 ggtcgtgcat gcccgtggcg gcgccggtga cgatcaggaa cctgtcggcg gcgagccggg
     8221 tgacggtgag gtcggccatg atgccgccgc gccaccccag catcgcggtg tagaccaccg
     8281 cccccggcgg ccggtcgatc tggctggcgc acagccactg cagaaggcgc gccgcgccgg
     8341 gccccgagag ctcgatcttg acgaacggcg acaggtcgaa cagcgccacg ccggcgcgcg
     8401 tcgccaggtg ctcggcgccc tcgaccggcg accagtggcg cgccgcccag ccgttgcgcc
     8461 cgggcacggc gaagcggtcg agcagcccct cgttggcggc gaaacaaagc gggcgctccc
     8521 atcccgccac ctcgaccatc tcgggatgca gcgcctcgag ccgcgcctgg aacgggctca
     8581 gccgcagccc gcgcggcgcc gcgatctgct ggcgcggatg gatgacgtcg tagacctcga
     8641 catattgctg ggcgccgcgc gcgcacacat aggcgggcgt ccgttgatga gcatggaaac
     8701 gattggcgtc ggcggcgtgc aggtcgatgc gcggctcgcc gtcgaccagc cagtcggcga
     8761 gcgcgcgcgc agcgccgccg gcatgggtca cccacaccgc cgcgccgagc cacagcccgt
     8821 cgacctctgt cggcccgagc agcggcaggc cgtcgggcgt gaacgagaac atgccgttga
     8881 tcgcatggtc gatccgcgca tcggcgacca gcggcagcag cgccttggcc tcggcgaagg
     8941 cgggcgcgaa atcggccgcg gtgaacgggc gcagcgccgt cgcgcccagg tcgtcggcct
     9001 cgaccaggcg cggggcgtgg cggtagctgc cgatgacata acggtcgccg acctggcgga
     9061 cgtagaggtc ctggtcctgg tggcgcagga tcgggtgggc gatctcgtcc tctcccgtcc
     9121 cggcgagcgc gggcaaggcg gtggtgcggg cgagctggtg ctcgaccgcg accagcggca
     9181 cggaaatccc ggcgagcttc ccgatccccg gcgaccacag gccggcgcag atcactgcga
     9241 cctcggtctc gatcgcgccg cagtcggtca gcaccgcgcg cagtcgcccc gccgacacct
     9301 cgaagccggt gacctcggtc tcgccctgga agcgcgccgc gcccgttgcc tccgcccggc
     9361 gggcgagcgc gcggcacagc cgcggcccgt cggcgacgcc gtcgcccggc acccacagcc
     9421 cgcccaggat gcgcccgcga tcgatcagcg ggagtcgcgc cgcgacctcg gccggcgtga
     9481 tcagcgcagc ctccgccact ccccagctcc gggcggagcc gagcttgcgg tggaggtcgg
     9541 cgagccgcgc gtcggtggtc gcgacctcaa tgccgccgac cgcgcgccag ccgggcgcgc
     9601 cgtcgagcct gagcgcgcga tagagacgga cgctcatccg cgcgagccgg gtgagcaggc
     9661 gcgacgagct ggtctggaac accagccccg gcgcatgccc ggtcgagccg ccggtgtcga
     9721 acagcggccc gcggtcgagc acgacgatgc cgtcgcggcc acgccgggcg aggtggtagg
     9781 cgagcgccga gccgacgatg ccggcgccga tcaccaccac ccgcgcgcgc tcggccatgg
     9841 cgcgcgccga tcaatccgac tttgacgccg cgccgaccac tgcgtcgagc aggcgggtcg
     9901 cggcggcggc ggggctcaag gtgccgctc
//
LOCUS       DASWHU010000279        11623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3125901, whole genome shotgun sequence.
ACCESSION   DASWHU010000279 DASWHU010000000
VERSION     DASWHU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125901"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGB88_10285"
     CDS             <1..236
                     /locus_tag="VGB88_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647878.1"
                     /translation="PAVPNTAAHAPANTVAALTIRLQDMTAIPRPVMSSPQTQLTRVM
                     PFKSNWPVGRPGFRPASCSFAAREPMMQPIPPS"
     gene            complement(333..620)
                     /locus_tag="VGB88_10290"
     CDS             complement(333..620)
                     /locus_tag="VGB88_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9647879.1"
                     /translation="MRNVLALYLRKSLISFEDALNLQAEAEALLHDKEYDVGSLDVLT
                     QAHQSPCSAYDCEFVALARSFRVKLVTMDRKILDAFPDTAVRLSDVAAGAF"
     gene            complement(738..980)
                     /locus_tag="VGB88_10295"
     CDS             complement(738..980)
                     /locus_tag="VGB88_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX9647880.1"
                     /translation="MATITLKNVPDDLYQRLKAQARAHRRPVNSELIRCLETVLRPTR
                     ISTEDRLRRFRGMRPRIDAHAVGAADIQQAIDEGRP"
     gene            complement(1088..1924)
                     /locus_tag="VGB88_10300"
     CDS             complement(1088..1924)
                     /locus_tag="VGB88_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX9647881.1"
                     /translation="MTGREASAACAAELRRLDYDRYLTALFARRPGRARLIALYAFNQ
                     EIARTAEMVSEPTLGAIRLQWWREAIEDAYQGRIRDHPVPQALAHAGTLELLSRDALH
                     ALIDARARDLDAAPFADRAALESYAAATSSRLIELALQALGVTGAGEAARRGGIAWAL
                     LGLMRAIPHLARQGRCPLPEALLAAHGIEPGAVLDGRVTESVRSAVREVAGWAGEHLA
                     AARENRVARPAVPALLPLALARLYRRRLERARFDPFAPTLVPSPLARQAALLQSHLRG
                     RP"
     gene            complement(1954..2331)
                     /locus_tag="VGB88_10305"
     CDS             complement(1954..2331)
                     /locus_tag="VGB88_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX9647882.1"
                     /translation="MADITPRLEQGRLVIEGYGGGGFRIAGVRHAGSVIVIADRVIRW
                     PVQDAQQLGLDDLAPVCAASGVEVLLIGCGARAVRIAPALRAGLGDAGISVDAMATGA
                     ACRTFNVLALEGRRVAAALIAVD"
     gene            complement(2340..3293)
                     /gene="secF"
                     /locus_tag="VGB88_10310"
     CDS             complement(2340..3293)
                     /gene="secF"
                     /locus_tag="VGB88_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9647883.1"
                     /translation="MFRLRLVPERTDIPFMAWRKYGFAASGALMVLSVVLVLVQGLNY
                     GIDFRGGILLEVKTPQPVDLGALRAQLTALGIGAIELQGFGEPDDVLIRIERQRGEEV
                     EQLAAVDAVKASLDQLYGAGVDYRRTEFVGPKVGSELIVAGVEAVAFALALMLVYIWF
                     RFEWQFGVGAVAALIHDVTLTVGMFALTGLEFNLTTVAAILLIVGYSMNDTVVVYDRV
                     RENLRKYKQMPLPELLSRSINDTLSRTLMTSLTTLLALVALYVLGGLVIRDFSFAMIW
                     GIVVGTYSSIFIAAPLLILLGLAKLGRGTEAAREEAASGGR"
     gene            complement(3310..4887)
                     /gene="secD"
                     /locus_tag="VGB88_10315"
     CDS             complement(3310..4887)
                     /gene="secD"
                     /locus_tag="VGB88_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9647884.1"
                     /translation="MLRFEPWKIALVAIVAAFGLLYALPNFLPRTLTEAVPGWLPSKQ
                     INLGLDLRGGSHLLAEVEVKAVVAKIMNTVEDDARAALRGARIGYTGLGNNGRQVTLT
                     LREPADAEAAAKLLGAIDRDLVVERGEGATMTVSLSETAAEERTRNAIEQAIEVIRRR
                     VDEMGTTEPTIQRQGEDRILIQVPGFDDPERLKEVIGRTASLDIRMVDEATPVEDALR
                     GRVPPGSEILYEPDPRGSGQEMPIVVRKRVEVTGENLTLAQTTVDQFGRPAVAIRFDT
                     VGARRFGKITTDNVGKSFAFVLDGKVVSAPVIREPIPGGNGQITGSFSVQEAETLALL
                     LRAGALPAPLTYLEERTVGPSLGADSIAAGKIASVVGLALVVVYMILTYGLFGIASTV
                     ALVVNIALIVALLSVLQATLTLPGIAGIVLTIGMAVDANVLIFERIREEVRNGRTPFS
                     AVDIGFQQALRTIIDANLTTLIAAVILYMFGSGPVRGFAVTLGIGIFTSVFTAVMVTR
                     LLIVLWLQRTRPQVLPV"
     gene            complement(4932..5285)
                     /gene="yajC"
                     /locus_tag="VGB88_10320"
     CDS             complement(4932..5285)
                     /gene="yajC"
                     /locus_tag="VGB88_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9647885.1"
                     /translation="MLISTALAQDGIGGGSGSGLIQLLPLVLIFVVFYFLLIRPQQKK
                     LKDHRRMVGELRRGDKVITAGGLHATVAKVEDDSDVLVLEIAPNVRVRAQRSTIAELA
                     GRREPRGANDDAKAE"
     gene            5480..6343
                     /locus_tag="VGB88_10325"
     CDS             5480..6343
                     /locus_tag="VGB88_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9647886.1"
                     /translation="MTVDRDLKQQLERIAAALDRLAPEAAPGNDLDAADAFVWQPDGR
                     RLQPVAEVSRVPLALLRGIDRVRDILLDNTRRFAARLPANNALLWGARGMGKSSLIKA
                     VHAAVAAETGGGLVLVEIHREDIAALPTLLDILRASARRFLLFCDDLSFDHDDTSYKS
                     LKGVLEGGIEGRPDNVVFYATSNRRHLMPRDMIENERSTAINPSEAVEEKVSLSDRFG
                     LWLGFHNCDQDTYLAIVRGYAEAYDLGADAETLRAEAVEWAATRGGRSGRVAWQYIQD
                     LAGRLGQRIAT"
     gene            6340..6600
                     /locus_tag="VGB88_10330"
     CDS             6340..6600
                     /locus_tag="VGB88_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9647887.1"
                     /translation="MSGKMRSIKASEFKAKCLKLIDEVAETGDPIVITKRGKPVVQVV
                     PAQRRAKPAQRRAKTVFGLHKGQIKILGDIIEPIDVEWEAER"
     gene            6830..9151
                     /locus_tag="VGB88_10335"
     CDS             6830..9151
                     /locus_tag="VGB88_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9647888.1"
                     /translation="MDEPNRKLAAILSCDVVGYSRLMQADEAATVDSLKKYRAAIGRV
                     VARHKGRVVNAPGDSILAEFPSAVEAVQAAVEIQKSVEGRNVEVAPDRRMQFRVGVNV
                     GDVIEEDDGTIYGDGVNIAARMEALADAGGICISSAVYDAVEGKLDFGFDFLGAQQVK
                     NIAKPVNVYRVRAEPRPAQAGHAKSGVDSRKALIGVAAAAVVVALAGVGVWQYRAAAP
                     PDGDAPPALALPDKPSIAVLPFANLSDDPEQEYFADGLTEDLITDLSKIPDLFVIARN
                     SSFTYKDKPTKVQQVAADLGVQYVLEGSVRRTGDAVRINAQLIDALSGHHLWAERYEG
                     AVAGIFDFHDQVLGQIVANLAVELAGTAPFGAASPAETEVVAAYDAFLTGRDFYRRRT
                     PQDYDRAIAAFEKAIELDPGYGRAYAALAGVYWDLTTLIWQLAIGTDFQHAYEQMLAN
                     LAKAKESPTADAYAVSAEMLARHGQHAEALADAERGIALEPNNPDIHISKARVLNVMG
                     RADEAEQSARLALRLDPHHQPDYLRVLSLALFHQERYDEAVRVMERVVARQPDVGDDY
                     ATLASAYGHLGRMKEARSAVENFNEFSENTTGRPLTVQERGTFWWYGDIYNYDPAYLA
                     RLQEGLRKAGVPEGPGEPGRHNEYRALMSKSNGEYSVAGSTKIDAAEAKALHDRAVTF
                     VDVRGSLNFRRGHIPGAEDLGATTVLSEQTLSERIGQNDEVVFYCFGKYCPLRAYACA
                     KALTWGYNRVYDFAGGFPAWEDAGYPVEQAPDI"
     gene            complement(9168..10091)
                     /locus_tag="VGB88_10340"
     CDS             complement(9168..10091)
                     /locus_tag="VGB88_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HEX9647889.1"
                     /translation="MIASRAVPPATVEVLDQGAAMTGRTTEVAAGDTLYAIARREDVA
                     IRSLIEANRLTPPYTLEPGRVLAVPALPEYQVRAGDTVYRISRCTGVDMAALTRLNGI
                     AEPYLISPGQRLRIPPPTHSPECPQRAHDDAFAVAAKPLPAPAPAAPAAEGEAAGPVQ
                     AAARVIPPPKPGPVPEPPPRAEGGFLWPTEGRVIARYGPQAGKRQNDGINIAAPQGAA
                     VRAAENGVIVYAGNELRGYGNLLLVRHADGWTSAYAHAAELLVGRGETVSRGQVIARV
                     GQTGNVAEPQLHFELRKGAEAVDPAEYLAND"
     gene            complement(10216..10863)
                     /locus_tag="VGB88_10345"
     CDS             complement(10216..10863)
                     /locus_tag="VGB88_10345"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9647890.1"
                     /translation="MEDPAARKARLILGLRRAGVTDREVLAAIEAVPRELFVPAGLAD
                     RAYDDTALPLGCGQTISQPTVVAWMTLALKVGERMKVLEIGTGSGYQSAVLARLCRRL
                     YTIERHRELLKAAEVRFEALNLHNITARVGDGAAGWPEQAPFERIIVTAAAREPPSAL
                     LDQLAEGGIMVLPMGPPDEEQTLVRIRKDKDGFHRERIFEVRFVPLIEGPLEERR"
     gene            complement(10880..>11623)
                     /gene="surE"
                     /locus_tag="VGB88_10350"
     CDS             complement(10880..>11623)
                     /gene="surE"
                     /locus_tag="VGB88_10350"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9647891.1"
                     /translation="LLSNDDGIQAPGLALLEKIMRGFTDDVWVVAPESDNSGAAHSLT
                     LAQPVRLRKRGERAYAVSGTPTDSVLLAVLELMTDRRPDLMLSGINRGGNLGEDITYS
                     GTVAAAMEASLLGIPAIALSQNRSGSGRTRWHTAEHHAPDLIRRLVAHGWPANVLINV
                     NFPDVEADRVEGVAVTRQGRRKPGSKLERRNDPRGRPYYWIDSARTEEPTLEGSDLSA
                     IYAHKISVTPLHLDLTHHETCVALGAALA"
BASE COUNT         1814 a         4111 c         4000 g         1698 t
ORIGIN      
        1 caccggccgt gccgaatacg gcggcacacg cgcccgccaa cacggtggcg gcactaacaa
       61 ttcgcttgca agacatgact gcaattcctc gcccggtgat gtcgtccccg caaacgcaac
      121 ttacccgcgt catgccgttc aagtctaact ggcctgttgg tcgcccaggt tttcggcccg
      181 caagctgctc ttttgccgct cgtgaaccca tgatgcagcc gatcccgcca agttgacagg
      241 acaatcgggg ccaccgtgcc gctctccacg cgatccgggc caagaatcgc ccctgcccgc
      301 tccgaggcgc ttcgcggatc gtcggggatg gcctagaacg cgcccgccgc gacatcgctc
      361 agcctgacgg cggtgtccgg aaacgcatcg aggatcttcc ggtccatggt caccagcttg
      421 actcggaagc tccgtgccag cgcgacaaac tcgcagtcat acgccgaaca ggggctttga
      481 tgcgcctgcg tgagcacgtc gagcgaaccc acatcatact ccttgtcgtg aagaagggct
      541 tccgcctcgg cctgaaggtt caaggcgtcc tcgaagctga ttagggactt gcgcaggtat
      601 aaggcaagca cgttgcgcat ctcgctgcgc cacaaggacg gcgatccaat cagggtccgt
      661 gagcaggagc tgctccgcca tcgcggtgta aggggacggg agcgccagaa acgcgataat
      721 gttgatgtcg gcgacgatca cggccggcct tcgtctatgg cttgctggat gtccgcggcg
      781 ccgaccgcgt gcgcatcgat ccgcgggcgc atgccgcgaa accgcctgag gcggtcctcg
      841 gtggagatcc tcgtcggcct caagaccgtc tcgaggcagc ggatcagctc gctgttgacg
      901 ggccggcgat gtgcccgggc ctgcgctttc aggcgctgat aaagatcgtc gggcacgttc
      961 ttgagcgtga tggtggccac cgggagtcct ccttgcgcct tgcgaaacca taatgtttca
     1021 ttttggattc tattcaaccg gacttaccgc gccggctttt ggccaatgcc aacgtcgccg
     1081 cggcacctca cggccggccg cggaggtggc tctggagcag ggcggcctgg cgcgcgagcg
     1141 gcgacggcac gagcgtgggc gcaaacgggt cgaagcgcgc gcgctcaagg cggcgccgat
     1201 agaggcgggc cagcgccagc ggcagcagcg ccggcacggc ggggcgcgcg acccgattct
     1261 cgcgcgccgc ggccaagtgc tcgcccgccc agccggcgac ctcgcgcacc gcgctcctca
     1321 cactctcggt gacacgcccg tcgaggaccg cgcccggctc gatgccgtgg gcggcgagca
     1381 gcgcctccgg cagcggacag cgcccctgtc gagccagatg cgggatcgcg cgcatgagcc
     1441 cgagcagcgc ccaggcaatg ccgccccgcc gcgcggcctc gcccgcgccc gtgacgccga
     1501 gcgcctgcag cgcaagctcg atcagccgcg acgatgtcgc cgcggcgtaa gactcgagcg
     1561 cggcgcggtc ggcgaacggt gccgcatcca ggtcgcgcgc gcgggcgtcg atcagggcgt
     1621 gcagcgcatc gcgcgacagc agctcgagcg tgcccgcgtg ggcgagcgcc tgcggcaccg
     1681 ggtggtcgcg gatgcggccc tgatacgcat cctcgatggc ctcgcgccac cattgcaggc
     1741 ggatcgcgcc gagcgtcggc tcgctcacca tctcggcggt gcgtgcgatc tcctggttga
     1801 aggcgtagag cgcgatcagg cgcgcacgcc ccggccggcg ggcgaacagc gccgtcaggt
     1861 agcggtcata gtcgagccgg cggagctccg ccgcgcacgc cgccgacgcc tcgcgcccgg
     1921 tcatggccat cgcggcgcgg cccggccggc gcgctagtcg accgcgatca gcgccgccgc
     1981 cacccgccgc ccctcgagcg cgagcacgtt gaaggtgcgg caggcggcgc cggtggccat
     2041 cgcgtcgacc gagatgcccg catccccgag ccccgcgcgc agcgccggcg caatgcgcac
     2101 cgcacgcgcc ccgcagccga tcagcagcac ctcgactccg ctcgccgcgc acaccggcgc
     2161 gaggtcgtcg agcccgagct gctgcgcatc ctgcaccggc caccggatca cccggtcggc
     2221 gatcacgatc accgagccgg catggcggac gccggcaatc ctgaagccgc ccccgccgta
     2281 gccttcgatg accaggcgcc cctgctcgag ccgcggggtg atatcggcca tagcgccgat
     2341 cagcgaccgc cgctggccgc ctcctcgcgc gccgcctcgg tgccgcgccc gagcttcgcg
     2401 agcccgagca ggatcagcag cggcgcggcg atgaagatcg acgagtaggt gccgaccacg
     2461 atgccccaga tcatggcgaa ggagaagtcg cggatcacca gcccgcccag cacgtacagc
     2521 gcgaccagcg cgagcaaggt ggtcagcgag gtcatcaggg tccgcgacag ggtgtcgttg
     2581 atgcttcggc tcaacagctc gggcagcggc atctgcttgt acttgcgcag gttctcgcgc
     2641 acccggtcgt acaccaccac ggtgtcgttc atcgagtagc cgacgatcag caggatcgcg
     2701 gcgacggtgg tgagattgaa ctcgagcccg gtgagcgcga acatgccgac ggtgagcgtc
     2761 acgtcgtgga tcagcgccgc caccgcgccg acgccgaact gccattcgaa ccggaaccag
     2821 atgtagacca gcatcagggc gagcgcgaac gccaccgcct cgacgccggc gacgatcagc
     2881 tcggagccga ccttgggccc gacgaactcg gtgcgccggt aatcgacgcc ggcaccgtag
     2941 agctgatcga ggctcgcctt gaccgcatcg accgcggcca gctgctccac ctcctcgccc
     3001 cgctggcgct cgatccggat cagcacgtcg tccggctcgc cgaacccctg cagctcgatc
     3061 gcgccgatgc cgagcgcggt cagctgcgcg cgcaacgcgc cgaggtcgac cggctgcggg
     3121 gtcttgacct ccagcaggat gccgccgcga aagtcgatgc cgtagttgag gccttgcacc
     3181 agcaccagca ccaccgacag caccatcagc gcgccggagg cggcgaagcc gtacttgcgc
     3241 cacgccatga acgggatgtc ggtccgctcg ggcaccaatc gcaggcgaaa catcaccggg
     3301 cctccgccgt cataccggca acacttgcgg gcgggtccgc tgcagccaca gcacgatcag
     3361 caggcgggtg accatgaccg cggtgaacac cgaggtgaag atgccgatgc cgagcgtgac
     3421 cgcgaacccg cgcaccggcc ccgagccgaa catgtacagg atgaccgccg cgatcaaggt
     3481 ggtcaggttg gcgtcgatga tcgtgcgcaa cgcctgctgg aagccgatat cgaccgccga
     3541 gaacggcgtg cggccgttgc gcacctcctc gcggatgcgc tcgaagatca gcacgttggc
     3601 gtcgaccgcc atgccgatag tcagcacgat gccggcgatg ccgggcaagg tcagcgtcgc
     3661 ctggagcacc gacaacaggg ccacgatcag cgctatgttg accaccagcg cgacggtcga
     3721 ggcgatgccg aacaggccgt aggtcaggat catgtacacg accaccagcg ccagcccgac
     3781 caccgaggcg atcttgcccg cggcgatcga gtcggcgccg aggctcgggc ccacggtccg
     3841 ctcctcgaga taggtgagcg gcgccggcag ggcgccggcg cgcagcagca gcgccaaggt
     3901 ctccgcctcc tggaccgaga agctgccggt gatctggccg ttgccgcccg ggatcggctc
     3961 gcggatcacg ggcgcgctca ccaccttgcc gtcgaggacg aaggcgaagc tcttgccgac
     4021 attgtcggtc gtgatcttgc cgaacctgcg cgcgccgacc gtatcgaagc ggatcgcgac
     4081 cgcgggccgg ccgaactggt cgaccgtggt ctgggccagg gtcaggtttt cgccggtgac
     4141 ctcgacccgc ttgcgcacca cgatcggcat ctcctggccc gacccgcgcg ggtcgggctc
     4201 gtacaggatc tccgagcccg gcggcactcg cccgcgcagc gcatcctcga ccggggtcgc
     4261 ctcgtcgacc atgcggatgt cgaggctcgc ggtccggccg atgacctcct tcagccgctc
     4321 cgggtcgtcg aagcccggca cctggatcag gatgcggtcc tcgccctggc gctggatggt
     4381 cggctcggta gtgcccatct cgtcgacccg ccggcggatc acctcgatcg cctgctcgat
     4441 cgcgttgcgc gtgcgctcct cggccgccgt ctcgctcagc gacacggtca tcgtggcgcc
     4501 ctcgccccgc tccacgacca ggtcgcgatc gatcgcgccg agcagcttcg cggccgcctc
     4561 cgcgtcggcc ggctcgcgca aggtcagcgt cacctgccgg ccgttgttgc cgagaccggt
     4621 atagccgatg cgcgcgccgc gcaacgccgc gcgggcgtcg tcctcgaccg tattcatgat
     4681 cttggcgacc accgccttga cctcgacctc ggccagcagg tgcgagccgc ccctgaggtc
     4741 gaggccgaga ttgatctgct tgctgggcag ccagcccggc accgcctcgg tcaaggtgcg
     4801 cggcaggaag ttgggcagcg catagagcag gccgaaggcc gcgacgatgg cgaccagggc
     4861 gatcttccag ggctcgaaac gaagcatcct acgggctccg cggaggggtc ggcggcgggg
     4921 cggtcaggcg atcactccgc cttggcgtcg tcgttggcgc cgcgcggctc acgccggccg
     4981 gccagctccg cgatggtact gcgctgcgcg cgcacgcgca cgttgggggc gatctcgagc
     5041 accagcacgt cgctgtcgtc ctcgaccttg gcgacggtgg cgtgaagccc gccggcggtg
     5101 atcaccttgt cgccgcgcct gagctcgccg accatccgcc ggtggtcctt gagcttcttc
     5161 tgctgcgggc ggatcagcag gaaatagaac accacgaaga tcaggaccag cggcaacaac
     5221 tggatcaggc cgctgccgct gccgccgccg atgccatcct gcgccaaggc cgtggagatc
     5281 agcatattgt cgggggactc cgatgcgttc ggccgctccc acgccggccg cgggatggcc
     5341 ggactatagc gatcgttggc gccattgcaa ctcgcccccc ggcgccgcgc cggccccgat
     5401 tgacgcccgg acgatctgtg ttaccgtccg cgccggccgc cgccctgcgc ctgcgggcgc
     5461 ccagcgagac gaggcccgca tgaccgtcga tcgagacctc aagcagcagc tcgagcgcat
     5521 cgccgcggcg ctcgaccggc tcgcgcccga ggccgcgccg ggcaacgatc tcgatgccgc
     5581 cgacgccttc gtctggcagc cggacggccg ccgcctgcag ccggtggccg aggtcagccg
     5641 ggtgccgctc gcgctgctcc gcggcatcga ccgggtgcgc gacatcctgc tcgacaacac
     5701 ccgtcgcttc gccgcccgac tgccggccaa caacgcgctc ttgtggggcg cccgcggcat
     5761 gggcaagagc agcctgatca aggcggtgca cgcggcggtc gcggccgaga ccggcggcgg
     5821 cctggtgctg gtcgagatcc atcgcgagga catcgccgcg ctccccacgc tactcgacat
     5881 cctgcgcgcc tccgcgcgcc gcttcctgct gttctgcgac gacctgtcgt tcgaccacga
     5941 cgacacctcg tacaagtcgc tcaagggcgt gctcgagggc ggcatcgagg gccggccgga
     6001 caacgtggtg ttctacgcca cctcgaaccg gcgccacctg atgccgcgcg acatgatcga
     6061 gaacgagcgc tcgaccgcga tcaacccgtc cgaggcggtc gaggagaagg tgtcgctgtc
     6121 cgaccgcttc ggcctgtggc tgggttttca caactgcgac caggacacct atctcgccat
     6181 cgtgcgcggc tacgccgagg cctacgatct cggcgccgac gccgagacgc tccgtgccga
     6241 ggcggtcgag tgggccgcga cccgcggcgg ccgctccggc cgggtcgcct ggcagtacat
     6301 ccaggacctc gccggccgcc tcggccagcg aattgcgaca tgagcggcaa gatgcgttcg
     6361 atcaaagcct cagagttcaa agccaagtgc ctgaaactca tcgatgaagt cgcggagacg
     6421 ggcgatccga tcgtgattac gaagcgcggc aagccggtgg tccaggtggt gccggcgcag
     6481 cgacgcgcaa agccggcgca gcgacgcgca aagaccgtgt tcggcctcca taaggggcag
     6541 atcaagatcc tcggcgacat catcgagccg atcgatgtcg agtgggaggc cgagcgctga
     6601 ccgcgaaaac ggccacgtat cggcgccgcc cgggcggacc aacggcggag cccgacgcgc
     6661 gcgcctgagc cggtcgatcc gccggcgaga ctggggaccg aacgaaaatc cgatggcctg
     6721 gccctgccga tttcgtgcta ccctcccggc tgcctgatgc ggccggggcg gcatggcccg
     6781 gtcgcagagc gaagtcgcgt ggccacgagc cgcggggacg gagcggctca tggatgagcc
     6841 gaaccggaag ctcgccgcca ttctgagctg cgacgtggtc ggttactcgc gcctgatgca
     6901 ggcggacgag gcggccacgg tcgactcgct caagaagtac cgggcggcga tcgggcgcgt
     6961 ggtcgcgcgg cacaagggcc gcgtcgtcaa cgcccccggc gattcgatcc tcgccgagtt
     7021 cccgagcgcg gtcgaggcag tccaggccgc ggttgagatc cagaagagcg tcgagggccg
     7081 caacgtggag gtcgcgcccg accggcgcat gcagttccgg gtcggcgtca atgtcggcga
     7141 cgtgatcgag gaggacgacg gcaccatcta cggcgacggc gtcaacatcg ccgcgcgcat
     7201 ggaggcgttg gccgacgccg gcggcatctg catctcgagc gcggtctacg acgcggtcga
     7261 gggcaagctt gatttcggct tcgactttct cggcgcccag caggtcaaga acatcgccaa
     7321 gccggtcaac gtctaccggg tccgcgccga gcccaggccg gcgcaggccg gccatgcgaa
     7381 gtcgggcgtt gacagcagaa aggccttgat cggggttgcc gcggcggcgg tggtggtcgc
     7441 gctcgcgggc gtcggcgtct ggcagtaccg tgcggccgcg ccgccggacg gcgacgcgcc
     7501 gccggcgctg gcgctgcccg acaagccgtc gatcgcggtg ctcccctttg ccaatctttc
     7561 ggacgacccc gagcaggagt atttcgccga cgggctgacc gaggacctga tcaccgacct
     7621 gtcgaagatc cccgatctgt tcgtcatcgc ccgcaactcg tcgttcacct acaaggacaa
     7681 gcccaccaaa gtgcagcagg tggccgccga cctcggcgtc cagtacgtgc tcgagggcag
     7741 cgtccggcgg accggggatg cggtgcggat caacgcccag ctgatcgacg cgctgagcgg
     7801 ccaccatctg tgggccgagc gttacgaagg cgccgtcgcc ggcatcttcg atttccacga
     7861 ccaggtgctg ggccagatcg tcgccaacct ggccgtggag ctggccggca ccgccccgtt
     7921 cggggcagcc agccctgccg agaccgaggt ggtcgcggcc tacgacgcgt tcctgaccgg
     7981 ccgcgatttt tatcggcggc gaacgccgca ggattacgac agggccattg ctgcgttcga
     8041 aaaggcgatt gagcttgatc ccggctacgg ccgcgcctat gccgccctag ctggcgtcta
     8101 ctgggacctt acgaccctga tttggcagct cgccatcggc acggatttcc agcatgccta
     8161 cgagcagatg ctggcgaatc tggcaaaggc caaggaaagt ccgactgcgg atgcctatgc
     8221 ggtgtcggcc gagatgttgg ccaggcacgg gcagcacgcc gaggcactcg ccgatgccga
     8281 acgaggcatc gcgctcgaac ccaacaaccc ggacatccac atcagcaagg ccagggtgct
     8341 caacgtgatg ggccgggcgg acgaggccga gcagagcgcg cgcctggcgc tcaggctcga
     8401 tccccatcac cagcccgact acctgcgcgt gctcagcctg gcattgtttc accaggaacg
     8461 ctacgacgag gcggtgcggg tgatggagcg cgtggtggct cggcagccgg acgtgggcga
     8521 cgactacgcg acgcttgcgt cggcctacgg ccatctcggc cgcatgaagg aggcccggtc
     8581 ggcggtcgag aactttaatg agttttctga gaacactacc ggccgcccgc tgaccgtgca
     8641 ggaacggggt acgttctggt ggtacggcga tatttacaac tacgaccccg cgtatctcgc
     8701 gcgtctacag gaaggattgc gcaaggctgg cgtcccggag ggcccaggcg aaccgggacg
     8761 gcataacgaa tatcgcgctc tcatgagtaa gagcaacggc gagtacagtg tcgccggtag
     8821 caccaagatc gacgccgccg aggccaaagc cctgcatgac cgcgccgtga catttgtcga
     8881 tgttcgcggc tcgctcaact tccgtcgcgg ccacatccct ggcgcggagg acctcggggc
     8941 aaccaccgtt ctgtcggagc aaaccctgtc tgaacgtatc ggccagaacg acgaggtggt
     9001 gttttactgc ttcggcaaat attgccccct tcgtgcctat gcctgcgcca aggcgctgac
     9061 ctggggttac aatcgggtct acgatttcgc cggcggcttc cccgcctggg aggacgccgg
     9121 ctacccggtc gagcaagcgc cggatattta gcgggcgcct tgctcgccta atcgttggcc
     9181 aaatactcgg ccggatcgac ggcctcggcg ccttttctga gctcgaagtg gagctgcggc
     9241 tcggcgacgt tgccggtctg gccgacgcgg gcgatcacct ggccgcggct caccgtctcg
     9301 ccgcgcccga ccagaagctc ggcggcatgc gcgtaggccg aggtccagcc gtcggcatgg
     9361 cgcaccaaca gtaaatttcc gtagcccctg agctcgttgc cggcatagac gatgaccccg
     9421 ttctcggccg cgcgcaccgc cgcgccctga ggggcggcga tgttgatgcc gtcgttctgg
     9481 cgcttgcccg cctgcgggcc gtagcgcgcg atcacccgcc cctccgtcgg ccacaggaag
     9541 ccgccctcgg cgcgcggcgg cggctcgggc accgggccgg gcttgggcgg cgggatcacc
     9601 cgcgccgcgg cctggacggg ccctgccgcc tcgccctccg ccgcgggcgc ggccggggcc
     9661 ggcgcgggca atggcttggc ggcgacggcg aacgcgtcgt cgtgcgcgcg ctgcgggcat
     9721 tccgggctgt gcgtcggcgg cgggatgcgt agccgctggc cgggcgagat gaggtagggc
     9781 tcggcgatgc cgttgagccg cgtgagcgcc gccatgtcga cgccggtgca gcgcgagatg
     9841 cggtagaccg tgtcgcccgc gcgcacctgg tactcgggca gcgccggcac cgccagcacc
     9901 cggccgggct cgagggtgta gggcggagtc aggcggttgg cctcgatcag gctgcggatg
     9961 gcgacgtcct cgcgccgggc gatggcgtag agggtgtcgc cggccgccac ctcggtggtg
    10021 cgccccgtca tggcggcgcc ctggtcaagc acctcgaccg tcgccggcgg gaccgcgcgc
    10081 gaggcgatca ccggcgcggg ctccggccac cgctcgaggc accccgccgt cgtcgcggcg
    10141 agcacggcgc caagcgcaac cgcccgcgcg gcgcgccagg cacgactcgt tccgaccctg
    10201 gactccgcgc ccaccctacc ggcgctcctc gagcgggccc tcgatcaggg gcacgaacct
    10261 gacctcgaaa atgcgctcgc ggtggaagcc gtccttgtcc ttgcgaatcc gcaccagcgt
    10321 ctgctcctcg tccggcggac ccatcggcag caccatgata ccgccctcgg ccagctggtc
    10381 gagcagcgcc gacggcggtt ccctggccgc ggcggtgact atgatccgct cgaacggcgc
    10441 ctgctcgggc cagcccgcgg cgccgtcgcc gacccgggcc gtgatgttgt ggaggttgag
    10501 cgcctcgaac cgcacctccg cggccttcaa cagctcgcgg tgccgctcga tggtgtacag
    10561 ccgccggcac agcctggcca gcaccgccga ctgatagccg ctgccggtgc cgatctcgag
    10621 caccttcatg cgctcgccga ccttgagcgc cagggtcatc caggcgacca cggttggctg
    10681 gctaatggtc tggccgcagc ccaatggcag cgcggtgtcg tcgtaggcgc ggtcggcgag
    10741 ccccgcgggc acgaacagct cgcgcggcac cgcctcgatc gccgccagca cctcgcggtc
    10801 ggtgacgccg gcgcgcctga ggcctaggat caggcgcgcc ttgcgtgcgg cagggtcttc
    10861 catgaaccgc ctccgcagct cacgccagcg cggcaccgag cgcgacacag gtctcgtggt
    10921 gggtgaggtc gagatggagc ggcgtgaccg agatcttgtg ggcatagatc gccgacaggt
    10981 ccgagccctc cagggtcggc tcctcggtgc gcgccgagtc gatccagtaa tagggccgcc
    11041 cgcgcgggtc gttgcgccgc tcgagcttcg agcccggctt gcgccggccc tggcgggtga
    11101 ccgcgacgcc ctcgacgcgg tcggcctcga catcggggaa gttgacgttg atcagcacgt
    11161 tggccggcca gccgtgggcg accaggcggc ggatcaggtc cggcgcgtgg tgctcggcgg
    11221 tgtgccagcg cgtccgcccc gagccgctgc ggttctggct cagcgcgatc gccgggatgc
    11281 cgagcagcga ggcctccatc gccgcggcga cggtgcccga ataggtgatg tcctcgccca
    11341 ggttgccgcc gcggttgatc cccgacagca tcaggtcggg gcgcctgtcg gtcatcagct
    11401 cgagcaccgc gagcagcacc gaatcggtcg gcgtgccgga caccgcgtag gcgcgctcgc
    11461 cgcgcttgcg caggcgcacc ggctgggcga gggtgagcga gtgggcggcg ccgctgttgt
    11521 cgctttccgg cgcgaccacc cagacgtcgt cggtgaagcc gcgcatgatc ttctcgagca
    11581 gcgccagccc cggcgcctgg atgccgtcgt cgttcgacag cag
//
LOCUS       DASWHU010000280         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2393264, whole genome shotgun sequence.
ACCESSION   DASWHU010000280 DASWHU010000000
VERSION     DASWHU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393264"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGB88_10355"
     CDS             <1..292
                     /locus_tag="VGB88_10355"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX9647892.1"
                     /translation="GAIAGGVVGDYLTTRDKELAGQTTTTALETQPAGTQSTWQNPDS
                     GNSGSITINDTFEKADGTPCRHFTQTVNAGGETNSGVGTACRAADGTWQIEG"
     gene            complement(289..1329)
                     /locus_tag="VGB88_10360"
     CDS             complement(289..1329)
                     /locus_tag="VGB88_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX9647893.1"
                     /translation="MAKLLPTSLVGSYAQPEWLIDRTGLVGRFPPRVRARELWRVAPE
                     FLDEAQDDATLLAIRDQERAGLDIITDGEMRRESYSNRFATALEGVDIDNPGTALDRS
                     GHPNPVPRVVGRVSRKHPVEVEALKFLRANTDRLVKMTVPGPFTLSQQAQNDVYASER
                     ELALDYAAAVNAEIKDLFAAGADIVQIDEPYMQARPEQARAFGLEALNRALDGVAGPT
                     AVHICFGYAAIIHRRPPAYSFLPELEQSRAQQISIETAQSALDCAVLEELPTKTIILG
                     VLDLSDMAVETPATVAARIRRALPHVAAERLIIAPDCGLKYLRRDVAFAKMRAMADGA
                     AIVRGELAGSGG"
     gene            complement(1390..1602)
                     /locus_tag="VGB88_10365"
     CDS             complement(1390..1602)
                     /locus_tag="VGB88_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX9647894.1"
                     /translation="MTEPVISPCTGVCRLNPRTRLCEGCRRTLEEIAGWLDYTEERKR
                     AIIAALAHRDPFRDTGARSGGSDALP"
BASE COUNT          269 a          626 c          643 g          258 t
ORIGIN      
        1 cggtgcgatc gccggcggcg tggtcggcga ctacctgacc acgagggaca aggagctggc
       61 cgggcagacg acgaccaccg cgctcgagac ccagcccgcc ggcacgcagt ccacgtggca
      121 gaacccggac agcggcaaca gcggcagcat caccatcaac gacaccttcg agaaggccga
      181 cggcacgccg tgccggcact tcacccagac cgtgaacgcc ggcggcgaga ccaattccgg
      241 tgtcggcacc gcctgtcggg cggccgacgg gacctggcag atcgagggct agccgccgct
      301 gccggcgagc tcgccgcgca cgatggccgc gccgtcggcc atcgcccgca tcttggcgaa
      361 cgccacgtcg cggcgcaggt atttgaggcc gcagtccggg gcgatgatca ggcgctcggc
      421 ggccacgtgc ggcagcgcgc ggcggatgcg ggcggcgacg gtcgcaggcg tctcgaccgc
      481 catgtccgac aggtcgagca cgccgaggat gatggtcttg gtcggcagct cttcgagcac
      541 cgcgcaatcg agcgcagact gcgccgtctc gatcgagatc tgctgggcgc gcgactgctc
      601 gagttcgggc aggaacgagt aggcgggcgg gcgccggtgt atgatcgccg catagccaaa
      661 gcagatgtgg acggcggtcg ggccggcgac gccgtcgagt gcgcggttca gcgcctctag
      721 gccaaaggcg cgcgcctgct ccgggcgcgc ctgcatgtag ggctcgtcga tctggacgat
      781 gtcggcaccg gcggcgaaca ggtccttgat ctccgcgttg accgccgccg cgtagtcgag
      841 cgcgagctca cgctcgctgg cgtagacgtc gttctgcgcc tgctgcgata gggtgaacgg
      901 gccggggacg gtcatcttga ccaggcggtc ggtgttggcg cgcaggaact tcagcgcctc
      961 gacctcgacc gggtgcttgc gcgagaccct gccgaccacg cgcggcaccg ggttggggtg
     1021 gccgctgcgg tcgagcgcgg tgccgggatt gtcgatgtcg acgccctcga gcgcggtcgc
     1081 gaaccggttg gagtagctct cccggcgcat ctcgccgtcg gtgatgatgt cgaggccggc
     1141 gcgctcctgg tcgcggatcg cgagcaaggt cgcatcgtct tgcgcctcgt ccaggaactc
     1201 gggcgccacc cgccacagct cgcgcgcgcg cacccgcggc gggaagcgcc cgacaaggcc
     1261 ggtccggtcg atcagccact cgggctgcgc gtacgacccg accagcgagg tcggcagcag
     1321 cttggccatg gtcttccccc ttcgcggacc ctccgcaccg acccgcgtac aatgctcttc
     1381 gacctcgggc tatggcaacg cgtcgctgcc gcccgacctc gcgcccgtgt cacggaacgg
     1441 gtcgcggtgc gccagggcgg cgatgatcgc acgcttgcgc tcttccgtgt agtcgagcca
     1501 gccggcaatc tcctcgagcg tccgccggca gccctcgcac agccgggtgc gcgggttcag
     1561 ccggcagacg ccggtgcatg gcgagatcac gggttcggtc accggctcac ctcatagccg
     1621 gcgtgcgctg aaaagaaaag gcggcgaccc gaaggccgcc gcctcgacgc gagtttggtg
     1681 ccgagatcag gcctcggcca tcgccgcctt gatcgcctcg ggcgtgatcg gcagctcgcg
     1741 cacgcgcgcg cccagcgcac ccgtgtagcg agcgcgtcca ataatggtaa tccttg
//
LOCUS       DASWHU010000281        19559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2747530, whole genome shotgun sequence.
ACCESSION   DASWHU010000281 DASWHU010000000
VERSION     DASWHU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19559
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747530"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VGB88_10370"
     CDS             complement(<1..747)
                     /locus_tag="VGB88_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9647895.1"
                     /translation="MIKSGGPVQSGGERSDRAMSNGLSASAARTSPLAVRRLGRSGID
                     VTILSLGGAGLGDRYGAVSEEAAAQTIHRALALGINYIDNSPSYGPFERRLGSALAAL
                     GGLPPGVHLCTKVGNHPDRQGDFSAAATRWTVENSLRLLGLNAIAVVQVHGVYHVDMD
                     TVLAPGGTVDELERLRDEGKLRAIGLAVYGRDFHRRAIASGRFDFILTHDDYTLVRQT
                     DAPLLEEAAAAGVGVLLGRALLTGLLAGDDP"
     gene            1191..1361
                     /locus_tag="VGB88_10375"
     CDS             1191..1361
                     /locus_tag="VGB88_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647896.1"
                     /translation="MTVWVVIARDPAELDALGLGDQWHALPAADGAPPWTDDYSNILE
                     AIRWRGTDFTVR"
     gene            complement(1369..1443)
                     /locus_tag="VGB88_10380"
     tRNA            complement(1369..1443)
                     /locus_tag="VGB88_10380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1409..1411),aa:Gly,seq:ccc)
     gene            complement(1499..2023)
                     /locus_tag="VGB88_10385"
     CDS             complement(1499..2023)
                     /locus_tag="VGB88_10385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9647897.1"
                     /translation="MASLPGWHEKAVARDHDRGEFDCGVPDLNEYLARYARQNHDLHY
                     VTTFVAVPDDNPRRVLGYYSLCNAHVEFTNLPESLVHGLPRYPVPAVRLARLACDLSC
                     QGLGLGRQLLLCAAKRAIAVSELSGAVILLVDAKNEQAAAWYEKNDAICLLDDALRLV
                     LPLASVRAALESSG"
     gene            complement(2555..2629)
                     /locus_tag="VGB88_10390"
     tRNA            complement(2555..2629)
                     /locus_tag="VGB88_10390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2595..2597),aa:Gly,seq:ccc)
     gene            2823..3782
                     /locus_tag="VGB88_10395"
     CDS             2823..3782
                     /locus_tag="VGB88_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9647898.1"
                     /translation="MPSAAPLAKPTLDPAKFRDQRLTAKGERRAHVRLDALRTLWFNT
                     GTLCNLTCAACYIESSPTNDSLAYLSLAEVRGFLDDIAAHDLATGEIGFTGGEPFMNP
                     AFLDMVELCLERGLRVLVLTNAMRPMMKCADRLLPLAGRYGGRLVLRVSLDHYTEGLH
                     QTERGARSWGPAIEGLQWLHRHGFTVHVAGRLKWGEPEASMRAGYARLFARLGVGVAA
                     DDPIQLVLFPEMDEHADVSEITEACWGTLGVEPGTMMCAHSRMVIKRKGAARPVVVPC
                     TLLPYDAQFELGASLAEAAGSVFLNHPHCARFCVLGGGSCSAA"
     gene            3868..4287
                     /gene="apaG"
                     /locus_tag="VGB88_10400"
     CDS             3868..4287
                     /gene="apaG"
                     /locus_tag="VGB88_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX9647899.1"
                     /translation="MAQAADDGPSYAATTRAVRITVTPFYLADQSSPDNHHFVFAYQI
                     RIDNQRQDTVQLRSRYWRITDGNGRVQEVRGVGVVGEQPRLAPGESFEYTSGTPLATA
                     SGIMVGTYQMVTDAGEPFEVAIPAFSLDAPDAGRRLN"
     gene            4334..4951
                     /gene="folE"
                     /locus_tag="VGB88_10405"
     CDS             4334..4951
                     /gene="folE"
                     /locus_tag="VGB88_10405"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9647900.1"
                     /translation="MNEILRTPSGAVAEPARPSRHAAEDAVRTLIRWAGDDPGREGLL
                     GTPARLARAYEEFFSGYGEDPQDVLARTFEEVDGYDEMVVLRDIRFESHCEHHMAPII
                     GVAHVAYLPDHRVVGISKVARVVDIYARRLQIQEKMTVQIADAIDTVLKPKGVAVVIE
                     ATHQCMTTRGVHKPGVTMTTSRMIGAFRDDPTTRREFLSMIRLPE"
     gene            complement(5061..8222)
                     /locus_tag="VGB88_10410"
     CDS             complement(5061..8222)
                     /locus_tag="VGB88_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9647901.1"
                     /translation="MNSEGKGLIGLFARHRTAANLVMILMIVAGALSLKFHNTQFFPD
                     FEVDFITVSIDWPGASAADVEANIVEALEPELRFLDDVKTVLGTAREGVGQIAIEFEP
                     NADMQGALSAVENAVSQVTTLPEDAEQPVIKRIAHYDTVSRLVVSGPFPEASLKAIAK
                     RMREELLAEGIDKITLFGARDEEVWVDIDEATLLRLDLTLGEVAAVIGESSLDLPSGD
                     TRGGAEQQIRSLGLMRDAESLGGLEVKALDSGEKIRLADIAALSDGFEDGQPIALHDG
                     MPAVELHIQRAIGNDALKLAGIVKGYLERLRPTLPPTLSVIQYDVAASLIRDRINLLL
                     RNGLSGLVLVVGVLFVFLSAGVAFWVAAGIPVSVLAGLAVMLATGQSINMVSLFALIM
                     MLGIIVDDAIVVGEHALARRLAGMPGPDAAEAGARRMLPPVMAASLTTIAAFVPVALV
                     SDIIGQILRAIPLVVVAVLIASLIECFLVLPGHMRGVLSGDPARASRLRRRFNAGFEA
                     FRDGPFRRFVTMCVRWRYATIGATVAMLLVSVGLVIGGRIQFVFFPSPESDVVYANFS
                     FAPGTPRAQSEAMIAELDRSLKTADARLGGGEELVRFALAKIGVPVGGRGETQQANGD
                     HLGGMIVEMVPSELRAVRTGELIEAWRAEIEPLAGLETLTVQERQGGPPGRDLDIRLS
                     GAEPPVLKAAALDLRDLLTRYPGVSDIGDDLPWGKQELVLEVTPRGRALGFTTERVGR
                     QVRDAFEGAIARRFARGDEEVLVRVQLPRESVSAGSLESLYLRAPGGAEVPLSQVVDV
                     RETRGFAVISRENGAREVSVAAEIDESRTNPTELVGALGAGPLTELKARTGVGFRFAG
                     KAEEQGKTLGDMKTGAVAGLSAIYIILAWVFASFSRPIIVMSIIPFALVGAIFGHMAL
                     GFDLTILSLIGLLGLSGIVVNDSIILVSTIDERLGNGEPVHGAIVQGACDRLRAVILT
                     SLTTIGGLTPLLFETSLQAQFLKPMALTLVFGLLATTILVLIVVPALVAIQDDLSLRR
                     RRSAAPEIGQEQDLHRKQLEAAE"
     gene            complement(8219..9436)
                     /locus_tag="VGB88_10415"
     CDS             complement(8219..9436)
                     /locus_tag="VGB88_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9647902.1"
                     /translation="MPVLLIGAGIAAAGWLRATRPEPPQKAAEERSWLIETATVAFAD
                     VQPELRVYGEVVAGREVSLRALVAGEVQSVADGFVDGGEVAGGDALVIIDPFDYQQDL
                     IERRAQLDEARARRGEMAGRLDAERAMRGEAENQVAINRRDVERRESLLGGAVSERGL
                     DEARLTLSRAEAELILHRQAIGALEAQIEQQNAVLARLDAALARAQRALDDTMLKAPF
                     DGYLADTAAQPGERLGVGDRVARLIDPSRLEARFHISEDDFGRLFATADGAPERTARV
                     VWRVGDRALTFPAAIARLGSEIDPTTGGVTLYARVRGGAEGLRMLRPGAFVEVMVPDR
                     LFRQVARLPATALHGESTVYAVVDGRLEPRAVAQLRRVGEDVLVTGEIAAGEEIAITR
                     FTEIGPGVKVAVR"
     gene            complement(9519..10286)
                     /locus_tag="VGB88_10420"
     CDS             complement(9519..10286)
                     /locus_tag="VGB88_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647903.1"
                     /translation="MIEVRGLAVGFRRAGRLVEAVRGVDLSVAAGEALGLVGESGSGK
                     STVLRAIAGLVAGQGGEIRVCGETRAGVARGAAARALQMVFQDPYGSLHPRKTVHETL
                     AEPLRLHGLDAGAARIAEALARVALGPETAFRFPHQLSGGQRQRVAIARALALEPAVL
                     LLDEPTSALDASTQAEILALLGALRRARGLTYLLVSHDLAVVAELCDRVAVMRAGRIV
                     ETLPVGALEAGAVGHAYTRRLLEASLAGRLSEPESND"
     gene            complement(10283..11110)
                     /locus_tag="VGB88_10425"
     CDS             complement(10283..11110)
                     /locus_tag="VGB88_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9647904.1"
                     /translation="MTGNAGEAPLLVVDDLSVTLPSRGGPVPVLDGVGFSLGREKLGL
                     VGESGAGKSTVGRALLGLLPAGAGVRARRIALGPDELAGASPARLRQFRGRRMAMILQ
                     DARHALDPVMTVGAQIAEAVRAHARVDARAARQRGLALLESVHVADAARVWRAYPHEL
                     SGGLGQRAMIAMMLAAEPEVLIADEPTSALDVIARAGVLRVLDELVAARGMGLLLISH
                     DLHLVGRFCDRVLVMQAGRIVEALAAGALDQARHPYTRALLAARPRLLAGAHARAAP"
     gene            complement(11107..11967)
                     /locus_tag="VGB88_10430"
     CDS             complement(11107..11967)
                     /locus_tag="VGB88_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647905.1"
                     /translation="MPRSVIAARLARARSRTGEIARNPLAAAGLAVIVLLVAVAALAP
                     YLAPYDPQAQELAGRLAPPGPVHWLGTDELGRDIASRLIWGARVTLVIVALVAAISAP
                     LGLVLGCVAGYLGGWADVALMRLTDVFLAFPRLILALALAAALGPGIDNAVLAIALTS
                     WPVYARVARAEALVARSAEFVDAARLQGSGAWHIIRRQIIPLCVSSLVVRASLDMAGI
                     ILTAAGLGFLGLGARPPAPEWGAMIAAGREYLFDQWWVVAAPGCAILVVSLGFNLLGD
                     GLRDVLDPHA"
     gene            complement(11994..13022)
                     /locus_tag="VGB88_10435"
     CDS             complement(11994..13022)
                     /locus_tag="VGB88_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9647906.1"
                     /translation="MASASRRARAAAAARLAVGVATTLFGLVLVTFVIGRMMPIDPVL
                     AVVGDRASAATYAAERAEMGLDRPLPVQVWRYLRALAAGDLGTSVVTARPVIEDIGRY
                     FPATIELATVALALGLALGVPAGVIAAAHRGRWPDQVIRITSLLGYSMPAFWLGLILL
                     LVFYAELGWAAGPGRIEVFYEDLVTPVTGLLLIDAPLDGEWVILGNALAHMALPASVL
                     GLFSMAYIARMTRSFMIEQLGQEYILAARAKGLSEARVVWRHALGNALVPLLTVVALA
                     YGSLLEGSVLTETVFAWPGLGSYLTNSLFNADMNAVLGCTIVIGLVFLGLNLLADLIG
                     TLLDPRVE"
     gene            complement(13006..14556)
                     /locus_tag="VGB88_10440"
     CDS             complement(13006..14556)
                     /locus_tag="VGB88_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9647907.1"
                     /translation="MFAVLAAGDAAAATPRSVLVMAKSLDDLITLDPAEVFEFSGAEI
                     TANLYLRLFRPNPDDPAKPLGEAVSSWRASADSLSFEFEVRDRLPFASGRAMDAHDAV
                     FSLRRAVALNKAPSFILTQFGLSGDNVGERVRALDDRRFTITLDRAYAPSLVLNALSA
                     GIASVVDREAVLANADGDLGEGWLRNHAAGSGAFSLRRWQPGEYLLLDANPSYWQGPP
                     ALKRVAIRDIREPSTQRLLLERGDVDMARNLGPDQLAAVDGIDGIRLLVVPRARLFYL
                     ALNQKRPPLDRPAVRKALRYLVDYQGIAERLLGPAARVHQAFLPQGFLGALDDRPFDF
                     EPERARGLLSEAGLAGGFEARLDVRSDPIALRLAQAVQATMAEAGVRIEIVPGSAKQV
                     LTRYRARRHELFMGQWGPDYLDPHTNASTFARNPDNGDGAAERTLAWRNGWEIPELTA
                     RADAAVLERDPARRARLYQELQREVQADSPFIVLFQENEVIALSDRVEGFAAGITADQ
                     TSYHGLRK"
     gene            14679..15848
                     /gene="argE"
                     /locus_tag="VGB88_10445"
     CDS             14679..15848
                     /gene="argE"
                     /locus_tag="VGB88_10445"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEX9647908.1"
                     /translation="MAGPSVSPYQMIERLVSFDTTSRGSNLDLIGFVADYLAGYGIKA
                     TRLLDDAGAKANLFATIGPERDGGVVLSGHTDVVPVDDQDWDSDPFTVVRRGGRLYGR
                     GTADMKSFSAVVLALVPELIARGPKRPVHLALSYDEEVGCLGAPRLVELLRAASFAPA
                     AVIVGEPTGMKVVNRHKGVYRFKTSVTGLEAHSAHTDRGVSAILVAGRLIERLDRLAR
                     AARAAAEPGSGFDPPYHTLHVGLVEGGTAVNILARHCAFDWELRLLPGGDAARDFLDP
                     FEAFVTAEVLPEMHAVSSRCAVATAPVVGVEGLAPVADNPAEALARTLAGDNAPAGAI
                     SFGTEAGLFQRGGTPTVVCGPGSILQAHKANEYIEVAQVEACIAFMRRLIAWACD"
     gene            complement(15814..16563)
                     /locus_tag="VGB88_10450"
     CDS             complement(15814..16563)
                     /locus_tag="VGB88_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9647909.1"
                     /translation="MSQWLRSALFNLAFVGGSALLSVALLPTLIMPRAALVGAVRTWA
                     RAVMRLFTGLIGAGIEVRGLDRLPAGACIVACKHQSALETVALTALLTDPSFVIKREL
                     VLLPVMGWLFLKLRYAVVDRGAGPRALKRLLAQAARAKADGRPIVIFPEGTRVAPGES
                     RAYQPGVAALYQHLDLMVVPVALNSGLAWGRRRFVKQPTHTVIEFLEPIPPGLERGAF
                     MDRLRTAIEGASRALEGEAGVSRTPTRSAVA"
     gene            complement(16560..17207)
                     /locus_tag="VGB88_10455"
     CDS             complement(16560..17207)
                     /locus_tag="VGB88_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9647910.1"
                     /translation="MPPRTLDRRRRWRLVLVVALAGAAAWAVGFVRFAADLPTAVERP
                     DRTTDAIVVLTGGSGRLDEGLALLRQGRAQKLLVSGVFRGVEVDELLQLARRGDEALS
                     ARVALGHEAGDTEGNARETAEWMVAEGFASLRLVTGAYHMPRSLFEFRRAMPEVDLVA
                     HPVFPPAVRLDAWWRSPGTAGLLATEYSKLLLARTRAAATDLVARVFANEAQARP"
     gene            complement(17215..18081)
                     /locus_tag="VGB88_10460"
     CDS             complement(17215..18081)
                     /locus_tag="VGB88_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX9647911.1"
                     /translation="MARRKAAIMVGGGADRYLSWSIGLKAFFAALAVAALVGSVDMSA
                     RWHSGEMAVVTVQLPTGSKADAVAAALDTVSRTLGIASAEALAPEAVAALLEPWLGSG
                     NLPEDLPLPVVIDVRLVPGAAVDWPALEARLAEAAPSAVLDTGTAWVERLLDLMRLAQ
                     LFAAMILVLVAGVAAISVVFATRAGLAVHREAVELLYLLGAHDSFIARQFQWRALWTG
                     LRGGLGGVLPAVGVLFALGYAAGRLDAPLLPEAALGVRAWAALAAVPVVTAGLAMVTA
                     RMTVLGALARMP"
     gene            complement(18088..18768)
                     /gene="ftsE"
                     /locus_tag="VGB88_10465"
     CDS             complement(18088..18768)
                     /gene="ftsE"
                     /locus_tag="VGB88_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX9647912.1"
                     /translation="MVRFENVGMRYGLGPEVLQDISFTLEPGSFLFLTGPSGAGKSSL
                     LRLLYLGHRPSRGLVTLLGTGVNGADRATLAGLRQKIGIVFQEFRLFDHLSARQNVAL
                     PLRISGAREPDIAAPVRELLEWVGLGDKLDALPPTLSGGEKQRLAIARAVIRRPRLLL
                     ADEPTGNVDRTNGQRLLRLFEALNELGATVVIATHDERLVKDYGHRCLRLEDGRLAAP
                     AGARSAAE"
     gene            18913..>19559
                     /locus_tag="VGB88_10470"
     CDS             18913..>19559
                     /locus_tag="VGB88_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX9647913.1"
                     /translation="MILTCPSCETRFSVADDALGAMGRTVRCGTCRHQWFESPGGDAF
                     GALAYQSDDTEPETDFDSLFDTEPSYGSDIPASSQHPDDASEAPLAEDEWDSEDAPRP
                     AAPVLTAAPAFRPRQRSSGWLGWTAVASVVVATSAAAALLADLVIEVWPPSKRLYAMA
                     GLPFAGPGEGLEMRNITARIEPAGDGTRLVIEGNIVNVSREVRPVPLVRVVLRDDR"
BASE COUNT         3105 a         7214 c         6563 g         2677 t
ORIGIN      
        1 cgggtcatcg ccggcgagta gccccgtgag cagcgcccgt ccaaggagca caccgacgcc
       61 tgccgccgca gcctcttcaa gcagcggggc atcggtctgg cgcacgagcg tatagtcgtc
      121 gtgggtgaga atgaagtcga agcgcccgct cgcaatggcg cgtcggtgga agtcgcgccc
      181 gtacaccgcg aggccaatcg cccgcagctt gccctcatcc cgcagccgct ccaactcgtc
      241 cacggtgccg ccaggggcga gcacggtgtc catgtcgaca tgatacacgc cgtggacctg
      301 cacaacagca atggcgttga gccctaggag tcgcaggctg ttctcaaccg tccagcgcgt
      361 tgcggccgcc gagaagtctc cctgacggtc cgggtggttc cccaccttgg tgcacagatg
      421 caccccaggt ggaaggccgc caagcgcggc cagcgcgctg cccaggcgtc gttcaaacgg
      481 gccgtaagag ggagaattat caatgtaatt gatgcccagc gccagcgcgc ggtgaatcgt
      541 ctgcgctgcc gcctcttcgg acaccgctcc gtaccggtca cccaggcccg caccaccgag
      601 agagagaatc gtgacgtcta tgccagaccg ccccaatcgc cgcacagcaa ggggtgaggt
      661 tcgcgccgca gaagcactca atccattcga catggctctg tcagatcttt ctcctccgct
      721 ttgcacaggc ccccccgact tgatcatgtc gtatgatcgg cgtccgatcg atgtacggca
      781 gctccgtcgg ctcgtcaccc gcacggcagc gggcgtacct ccgccgctcc ggtctggagg
      841 ttacttcagt aggaatcccg cttgcccagc ggtaacccat tgtttcgtcg caggctgctc
      901 ctgtcaagat taccggagtt tcgaccgctg aaaccatgac cacgtgcgaa aggcgaaggt
      961 gtcaggcagc agacgatcac actgccggta atgcccggaa agaaaacgct accgaaaccg
     1021 gactgatact cgagaatagc ggttaacgaa tcgatccccc gcggcaccgt agacgacata
     1081 cgagcggtgc tggcgcccga ctggcaacga aactcggcct cgccgcgcgg gtcggcacca
     1141 ccgccttcgc cgacgccccg aagaccggcg ccggccatta cgccaccgcg atgaccgtgt
     1201 gggtggtgat cgcgcgcgac ccggcggagc tcgacgccct cggcctcggc gaccagtggc
     1261 acgcgctgcc cgccgctgac ggcgccccgc cgtggaccga cgactactcc aacatcctcg
     1321 aggcgatccg ttggcgcggc accgacttca ctgtccgcta gcggcagctg gagcgggcga
     1381 ccgggattga accggcgaca ttcagcttgg gaagctgacg ttctaccact gaactacgcc
     1441 cgcgcactgt cgccattctc ggcaaggctg gcggattgcc gccccggtgg ttactcagct
     1501 agcccgacga ttccagcgcc gcgcgcacgg acgccagcgg cagcaccagc cggagggcat
     1561 cgtcgagcag gcatattgcg tcgtttttct cgtaccaggc cgcggcctgc tcgtttttcg
     1621 catcgaccag gagtatgacg gcgccactca gctcggagac tgcaatcgcg cgcttcgcgg
     1681 cgcaaagaag cagttgacgc cccagtccga gcccttggca ggacagatcg cacgccagcc
     1741 tcgccaggcg cacggccggt accggatacc gagggaggcc gtggaccagg ctctcaggca
     1801 gattggtaaa ctccacatgg gcgttgcaga ggctgtagta gccgagaaca cgtcgtgggt
     1861 tgtcgtcagg cacggccacg aacgtggtga cgtaatgcaa gtcatgattt tgacgcgcgt
     1921 accgagcgag atactcgttg aggtccggta cgccgcaatc gaactcgcca cggtcgtgat
     1981 cacgcgcgac cgctttctcg tgccagccag gtagcgacgc cacctagccc cgagaggctt
     2041 tgattgcctt gagcagtccc ttggacagct tgggagggtc cttcaggagc gccgccacgc
     2101 gcttgctgtc gcgctcagac aagacgacgc attcgtcgct gcgtgggcgg cgagagaacc
     2161 acgagctcgc ccggctaaac gcgcgttccg ccctctgcat cagggccgtc attgtcccgt
     2221 ctccgaaacc atggcgtcag acccggcacg caaccgcttg gctgagagcg gcgcgttcgc
     2281 actaggttca cactagatat acctaccagg tgccggcttt caacatggga cgaggccaat
     2341 cacgccccgg cggcgtcgca cctcgcatca ttccgcgccg gccaattgat ccgacgtgtc
     2401 caactaacgt gctgatttta ttgaaggtgc gcagggacac gaccgcggtc cgccgaccga
     2461 gccccgccgt ggaccgacga ctactccaac atcctcgagg cgatccgctg gggcggcacc
     2521 gacttcgccc gccgggaccg gtagcggggg gcagtggagc gggcgaccgg gattgaaccg
     2581 gcgacattca gcttgggaag ctgacgttct accactgaac tacgcccgca gccgtggcga
     2641 gtctcgacgc ctgcggcaac gaagtaaagc cggaatcgtc gcccctcggc ccggttcacg
     2701 tccgcttgag cgtgcctgcg tcgcagttga caaaacccgg ccggcccgta ccgtcgctgc
     2761 cgaggcggcg cccgtggtgg ccgtccatcg cccgggtcac gaccacagga agaggcgccg
     2821 tcatgccatc cgccgccccg ctcgccaagc ccaccctcga tccggccaaa ttccgcgatc
     2881 agcgcctgac cgcgaaaggc gagcgccgcg cccatgttcg gctcgatgcc ttgcgcacgc
     2941 tgtggttcaa caccgggacc ttgtgcaacc tgacctgcgc cgcgtgctac atcgagtcga
     3001 gcccgaccaa cgacagcctg gcgtatctgt cgctcgccga ggtgcggggg ttcctcgacg
     3061 acatcgccgc gcacgacctt gccaccggcg agattggctt caccggcggc gagccgttca
     3121 tgaaccccgc gttcctcgac atggtcgagc tgtgcctcga gcgcggcttg cgggtgctgg
     3181 tgctgaccaa cgccatgcgg ccgatgatga agtgcgccga ccgcctgctg ccgctcgccg
     3241 gccgctacgg cgggcgcctg gtgctgcgcg tgtcgctcga ccactacacc gagggcctgc
     3301 accagacaga gcgcggcgcc cgcagctggg gcccggcgat cgagggcctg caatggctgc
     3361 atcgccacgg gttcacggtg cacgtcgcgg ggcggctcaa gtggggcgag cccgaggcct
     3421 cgatgcgcgc cggctacgcc cgcctgttcg cgcgcctcgg cgtcggcgtc gccgccgacg
     3481 accccatcca gctggtgctg ttccccgaga tggacgagca cgccgacgtg tccgagatca
     3541 ccgaggcctg ctggggcacc ctcggcgtcg agccggggac catgatgtgc gcccactcgc
     3601 gcatggtgat caagcgcaag ggcgccgccc ggccggtggt ggtgccctgc accctgctcc
     3661 cctacgacgc ccagttcgag ctcggcgcca gcctcgccga ggcggccggc tcggtattcc
     3721 tcaaccaccc gcattgcgcc cgcttctgcg tcctcggcgg cggctcgtgc agcgccgctt
     3781 gagcctgccg ccgggccgag gcccggctgg acaagcctgg cgccttggcg caccctttgt
     3841 gacgggtttc gaggagcttg accacagatg gcgcaggcag cagacgacgg cccgtcctac
     3901 gcggcgacca cgcgcgcggt ccggatcacc gtgacccctt tctatctggc cgaccagtcg
     3961 tcgcccgaca accaccattt cgtgttcgcc taccagatcc ggatcgacaa ccagcgccag
     4021 gatacggttc aactgcgctc gcggtactgg cgcatcaccg acggcaatgg ccgtgtccag
     4081 gaggtgcgcg gcgtcggcgt ggtcggcgag cagccgaggc tcgcgccagg cgagagcttc
     4141 gagtacacca gcggcacgcc gctggcgacc gcgagcggga tcatggtcgg cacctaccag
     4201 atggttaccg acgcgggcga gcccttcgag gtcgccatcc ccgccttctc gctcgatgcg
     4261 ccggacgccg gcaggcgctt gaactgagcc accccgcggg cgcacgcttg ctcaatcgcg
     4321 aggccagacc agaatgaacg agatcctccg aaccccgtcc ggcgccgtcg ccgagcccgc
     4381 ccgccccagc cgccacgcgg ccgaggacgc cgtgcggacg ctgatccgct gggccggcga
     4441 cgatcccggt cgcgagggcc tgctcggtac gccggcgcgg ctggcgcgcg cctatgagga
     4501 gttcttctcc ggctatggcg aggatccgca ggacgtcctg gcccgcacct tcgaggaggt
     4561 cgacggctac gacgagatgg tggtgctgcg cgacattcgc ttcgaatcgc actgcgagca
     4621 tcacatggcg ccgatcattg gcgtcgccca cgtcgcctac ctgcccgacc atcgcgtggt
     4681 cgggatcagc aaggttgccc gtgtggtcga catttatgcg cgccggctgc agatccagga
     4741 gaagatgacc gtccagatcg ccgacgcgat cgacacggtg ctcaagccca agggcgtggc
     4801 agtggtgatc gaggcgaccc accaatgcat gacgacgcgc ggggtgcaca agccgggcgt
     4861 gaccatgacc accagtcgga tgatcggggc gttccgcgac gaccccacca cccggcgcga
     4921 gttcctgagc atgatccgcc tgccggagta gctcctccgg cagcggcgca ccatcatcgc
     4981 cgcccctgat cgcggcgatc accactaccg gctccagttc ggccgattac ggcgcctggg
     5041 gcacgctgcc ggcgtcgatg ttactcggcc gcttcgagct gtttacggtg caggtcctgt
     5101 tcctgcccaa tttctggcgc cgctgaccgg cgccgccgca ggctgaggtc gtcctggatc
     5161 gcgaccagcg ccggcaccac gatcaggacc aggatcgtgg tcgcgagcag cccgaacacc
     5221 agggtcaacg ccatcggctt caggaactgc gcctgcaggc tggtctcgaa caacagcggc
     5281 gtcaggccgc cgatggtggt cagcgatgtc aggatcaccg cgcgcaggcg gtcgcaggcg
     5341 ccttggacga tggcgccgtg gaccggctcg ccgttgccca gccgctcgtc gatcgtgctc
     5401 accaggatga tcgagtcgtt gaccacgatc cccgacaggc ccaacaggcc gatcaggctc
     5461 agaatggtca ggtcgaagcc cagggccatg tgcccgaata ttgcgccgac cagagcgaac
     5521 gggatgatcg acatgacgat gatcggccgg ctgaagctcg cgaacaccca ggccaggatg
     5581 atgtagattg cgctcaggcc ggcgaccgca ccggtcttca tgtcgccgag ggtcttgccc
     5641 tgctcctcgg ccttgcccgc gaagcggaag ccgacgccgg tgcgcgcctt gagctcggtt
     5701 aatggaccgg cgccgagcgc gcccaccagc tcggtcgggt tggtccggga ctcatcgatc
     5761 tcggcggcca ccgacacctc gcgggcgccg ttctcgcggc tgatgaccgc gaagccgcgg
     5821 gtctcgcgca cgtcgaccac ctggctcaac ggcacttcgg cgccgccagg cgcgcgcagg
     5881 tacaggctct ccaacgagcc cgcgctcacc gactcgcgcg gcaactgcac gcgcaccagc
     5941 acctcttcgt cgccgcgcgc gaaacggcgc gcgatcgcgc cctcgaacgc gtcgcgcacc
     6001 tggcggccga cccgctcggt ggtaaagccg agcgcgcgcc cgcgcggggt tacctcgagc
     6061 accagctcct gcttgcccca cggcaggtcg tcgccgatgt cgctgacgcc ggggtagcgg
     6121 gtcagcaggt cgcgaagatc gagcgccgcc gccttcagca ccggcggctc ggcgcccgac
     6181 agccggatgt cgaggtcgcg ccccggcggc ccgccctggc gttcctggac ggtcaatgtc
     6241 tcgagcccgg ccagcggttc gatctcagcc cgccatgcct cgatcagctc gcccgtgcgg
     6301 accgcgcgca gctcggacgg caccatctcg acgatcatgc cgcccagatg gtcgccgttg
     6361 gcttgctggg tctcgccgcg accgccgacc gggacgccga ttttggcaag cgcgaaccgg
     6421 accagctcct cgccgccgcc aagccgcgca tccgccgttt tcaacgaccg atcgagctcc
     6481 gcgatcatcg cctcgctctg cgcgcgcggc gtgccgggcg cgaagctgaa gttcgcgtac
     6541 acgacgtcgg actcgggcga ggggaagaac acgaactgga tccgcccgcc gatcaccaac
     6601 ccgaccgaga ccagcagcat cgcgaccgtc gcgccaatgg tggcgtaacg ccagcgtacg
     6661 cacatggtca cgaaacggcg gaacgggccg tcgcggaacg cctcgaagcc ggcattgaac
     6721 cgtcgcctca gccgcgacgc ccgcgccggg tcgccggata gcacgccgcg catgtgcccg
     6781 ggcagcacca ggaagcattc gatcaggctg gcgatcagca ccgccaccac caccagcggg
     6841 atcgcgcgca ggatctggcc aatgatgtcc gacaccaggg caaccggcac gaacgcggca
     6901 atagtggtca gcgacgccgc catcaccggc ggcagcatcc gccgcgctcc cgcttccgcg
     6961 gcatccggcc cgggcatccc ggccagcctg cgcgccaacg catgctcgcc gaccacgatc
     7021 gcgtcgtcga cgatgatgcc cagcatcatg atcagcgcga acaggctgac catgttgatc
     7081 gactgcccgg tcgccagcat caccgccagc cccgccaaca ccgacaccgg aatgccggcc
     7141 gccacccaga acgcgacgcc cgcgctcagg aacacgaaca gcaccccgac gaccagcacc
     7201 aggccgctca ggccgttgcg cagcaaaagg ttgatgcggt cacgaatcag gctggcggcg
     7261 acgtcgtact ggatcaccga caaggtcggc ggcagggtcg ggcgcaacct ttcgagatag
     7321 cccttgacga tgccggcgag cttgagcgcg tcgttgccga tcgcacgctg gatatgaagc
     7381 tcgaccgcag gcatgccgtc atgcagcgcg atcggctggc cgtcctcgaa gccgtcgctc
     7441 aaggcggcaa tgtccgcgag ccggatcttc tcgccgctgt cgagagcctt gacctcgagt
     7501 ccgccgagcg actcggcgtc acgcatgagg ccgaggctgc ggatctgctg ctcggcaccg
     7561 ccgcgcgtat cgcccgaggg caggtccagg ctgctttcgc cgatcaccgc cgccacctcg
     7621 cccaaggtga ggtcgagccg cagcagcgtc gcctcgtcga tgtcaaccca cacctcctcg
     7681 tcgcgcgcgc cgaacagggt gatcttgtcg atgccttccg ccagcagctc ctcgcgcatg
     7741 cgcttggcga tcgctttcag cgacgcctcc gggaacgggc ccgagaccac cagccggctg
     7801 accgtgtcgt agtgggcgat gcgcttgatc accggctgct cggcgtcctc gggcaaggtg
     7861 gtcacctgcg acaccgcgtt ctcgaccgcc gacagggcgc cctgcatgtc ggcgttgggc
     7921 tcgaactcga tcgcgatctg gccgacgccc tcccgcgcgg tgccgagcac ggtcttgacg
     7981 tcgtcgagga agcgcagctc tggttccagt gcctcgacga tgttggcctc gacgtcggca
     8041 gcgctcgccc ccggccagtc gatgctgacg gtgatgaaat cgacctcgaa gtcggggaag
     8101 aactgggtgt tgtgaaattt cagcgacagc gcgccggcga cgatcatcag gatcatcacc
     8161 aggttggccg cggtccgatg gcgggcgaac agcccgatca ggcccttgcc ctcgctgttc
     8221 accgaacggc taccttgacg cccggaccga tttcggtgaa ccgggtgatg gcgatctctt
     8281 cgccggcggc gatctcgccg gtcaccagga cgtcctcgcc gacgcgcctg agttgcgcca
     8341 cggcgcgcgg ctcgaggcgg ccgtcgacca cggcgtagac cgtgctctca ccgtgcagtg
     8401 ccgtcgccgg caggcgcgcc acctggcgga acagccggtc gggaaccatc acctcgacga
     8461 atgcgcccgg ccgcagcatc cgcagacctt cggcgccgcc ccggacccgc gcgtacaggg
     8521 tcacgccgcc ggtggtcggg tcgatctcgc tgcccaggcg cgcgatcgcg gcggggaagg
     8581 tgagcgcccg gtcgccgacc cgccagacca cgcgcgcggt gcgctcgggc gcgccgtccg
     8641 cggttgcgaa caggcggccg aagtcgtcct cgctgatatg gaaccgggct tcgaggcggc
     8701 ttgggtcgat cagtcgcgcg acgcggtcgc ccacgcccag acgctcgcca ggctgcgccg
     8761 cggtatcggc cagatagccg tcgaacggtg ccttcagcat ggtgtcgtcc agcgcccgct
     8821 gggcgcgggc gagagcggcg tccaggcggg cgaggaccgc gttctgctgc tcgatctgcg
     8881 cttcgagcgc gccgatcgcc tggcgatgca ggatcaattc ggcctcggcg cggctgagcg
     8941 tgaggcgcgc ctcgtcgagg ccgcgctccg agaccgcccc gccgagcagg gactcgcggc
     9001 gttcgacgtc gcggcggttg atcgcgacct ggttctcggc ctcgccccgc atcgcccgct
     9061 cggcgtcgag ccgccccgcc atctcgcccc gtcgcgcgcg cgcctcgtcg agctgggcgc
     9121 ggcgctcgat caggtcctgc tgatagtcga acgggtcgat aatgaccagc gcgtcgccgc
     9181 ccgccacctc gccgccgtcg acgaagccgt cggcgacgct ctggacctcg cccgcgacca
     9241 gcgcccgcag gctcacttcg cgcccggcga ccacttcgcc gtagaccctg agctcgggct
     9301 ggacgtccgc gaacgcgacc gtcgccgtct cgatcagcca ggagcgttcc tcggccgcct
     9361 tttgcggcgg ctcgggcctg gtcgcgcgca gccagcctgc cgcggcgatc cccgcgccga
     9421 tcaggaggac aggcaccagg gctttgagaa atttacgcaa attccggtgt cccgagccgc
     9481 taaacccaca acataggggc cgcgccggaa gcgttcaatc agtcgttcga ctccggctcc
     9541 gagagccgcc cggcaaggct cgcctcgagc agcctccgag tataggcatg gccgaccgcg
     9601 ccggcctcga gcgcgccgac cggcaacgtc tcgacgatcc gccccgcccg catcaccgcc
     9661 acccggtcgc acagctcggc gaccaccgcc aggtcgtggc tgaccagcag gtaggtgagc
     9721 ccgcgcgcgc gccggagcgc acccagcaat gccaggatct cggcctgcgt cgaggcatcg
     9781 agcgccgagg tcggctcgtc gagcaacagc accgccggct cgagggcgag cgcacgggcg
     9841 atggccacgc gctggcgctg gccgcctgag agctggtggg gaaaccggaa cgccgtctcc
     9901 ggcccgagcg cgacccgggc gagcgcctct gcgatgcgcg cggccccggc atcgaggccg
     9961 tgcaggcgga gcggctcggc cagggtctcg tgcaccgtct tgcgcggatg aagcgagccg
    10021 taggggtcct ggaacaccat ctgcagcgcg cgcgccgctg cgccacgggc aactcccgca
    10081 cgggtttcgc cgcagaccct gatctcgccg ccttgccccg caaccaggcc cgcgatcgcg
    10141 cgcagcaccg tcgacttgcc cgagcccgac tcgccgacca ggccgagcgc ctcgcccgcc
    10201 gccaccgaca ggtcgacgcc gcgtaccgcc tcgaccagcc ggccggcgcg gcggaagccg
    10261 accgcgagcc cgcgcacctc gatcacggcg cggcccttgc gtgtgcgccg gcgagcagcc
    10321 gcggccgggc ggcgagcagc gcgcgggtgt aggggtggcg ggcctggtca agcgcaccgg
    10381 cggcgagcgc ctcgacgatc cgccccgcct gcatcaccag cacccggtcg cagaaccgcc
    10441 cgaccagatg caggtcgtgg ctgatcagca acagccccat gccgcgcgcc gcgaccagct
    10501 cgtcgagcac acggagcacg ccggcgcgcg cgatcacgtc gagcgccgag gtcggctcgt
    10561 cggcaatcag cacctcgggc tcggccgcca gcatcatcgc gatcatcgcc cgctggccga
    10621 ggccgcccga cagctcgtgc ggataggcac gccagacgcg cgccgcatcc gccacatgca
    10681 ccgattcaag cagcgcgagg ccacgctggc gggccgcgcg cgcatccacc cgggcgtggg
    10741 cgcgcacggc ttcggcaatc tgggcgccga ccgtcatcac cggatcgagc gcgtggcggg
    10801 cgtcctgcag gatcatcgcc atgcgccggc cgcggaattg ccgcaggcgc gcgggcgagg
    10861 cgccggcgag ctcgtcaggc ccgagcgcga tccggcgcgc gcgcacgcct gcacccgccg
    10921 gcaacagccc gagcagcgcg cgcccgaccg tcgacttgcc ggcccccgac tcgccgacca
    10981 ggccgagctt ctcgcgcccg aggctgaagc ccaccccatc cagcaccggc acgggcccgc
    11041 cgcgcgacgg cagcgtcacc gacaggtcgt ccaccaccag cagcggcgcc tcgcccgcgt
    11101 tgccggtcat gcgtgcgggt ccagcacgtc gcgcagcccg tcgccgagca ggttgaagcc
    11161 gaggctcacc accaggatcg cgcaaccggg ggccgccacc acccaccact ggtcgaacag
    11221 atattcgcgc ccggcggcga tcatcgcgcc ccattcgggc gccggcggcc tggcgccgag
    11281 cccgagaaag ccgagtcccg cggcggtcag gatgattccc gccatgtcga ggctcgcccg
    11341 caccaccagc gagctcacgc acagcggtat gatttggcgg cgaatgatat gccaggcacc
    11401 ggatccctgc aggcgcgcgg catcgacgaa ctcggcgctg cgcgcaacca gggcctcggc
    11461 gcgcgcgacg cgcgcataga ccggccacga tgtaagcgcg atcgcgagca ccgcgttgtc
    11521 gatcccgggc ccgagcgccg ccgccagcgc cagcgccagg atcagccgcg ggaacgcgag
    11581 aaacacgtcg gtgagccgca tcagggcgac gtcggcccag ccgccgagat agcctgcgac
    11641 gcaaccgagc acgagcccga gcggggcgct gatcgcggct accagggcga cgatgacgag
    11701 tgtcacgcgc gcgccccaga tcagccgcga ggcgatgtcg cggccgagct cgtcggtgcc
    11761 gagccagtgc acgggccccg gcggcgcaag ccgccccgcg agctcctgcg cctgcgggtc
    11821 gtacggcgcc aggtacgggg cgagcgccgc aaccgcgacc agcagcacga tcaccgccag
    11881 gccggccgca gccagcgggt tgcgcgcgat ctcgccggtg cgcgaccggg cgcgcgcgag
    11941 tcgtgccgca atcaccgagc gcggcatcga cgcggcgcgc tcggcctccg ccgtcattcc
    12001 accctcgggt cgagcaaagt gccgatcagg tcggcgagga ggttgaggcc caggaacacc
    12061 agcccgatca cgatcgtgca cccgagcacc gcgttcatgt cggcgttgaa cagcgagttg
    12121 gtgaggtagc tgccgagccc gggccaggcg aacaccgtct cggtcagcac cgagccctcg
    12181 agcaagctgc cataggcgag cgcgaccacg gtcaacagcg gtaccagggc gttgccgagc
    12241 gcgtgccgcc acaccacccg cgcctccgac agacccttgg cgcgggcggc caggatgtat
    12301 tcctggccca gctgctcgat catgaagctc cgcgtcatgc gcgcgatgta ggccatcgag
    12361 aacaggccga gcacgcttgc cggcagcgcc atgtgggcga gcgcgttgcc gaggatcacc
    12421 cactcgccgt cgagcggcgc gtcgatcagc agcaagccgg tcacgggcgt caccaggtcc
    12481 tcgtagaaca cctcgatccg ccccggcccg gccgcccagc cgagctcggc gtagaacacc
    12541 agcagcagga tcaagcccaa ccagaaggcg ggcatcgaat agccgagcaa cgatgttatc
    12601 cggatcacct ggtccggcca gcgcccgcga tgggcggccg caatcacacc cgccggcacg
    12661 ccgagcgcca gcccaagtgc gagcgcgacc gtcgccagct cgatggtcgc cgggaaatag
    12721 cggccgatgt cctcgatcac gggccgcgcc gtgaccacgc tggtgccgag atcgcccgcc
    12781 gccagcgctc gcagataccg ccagacctgc accggcagcg gccggtcgag acccatttcc
    12841 gcgcgctcgg cggcataggt cgcggcgctc gcccggtcgc cgaccaccgc cagcaccggg
    12901 tcgatcggca tcatgcgtcc gatcacgaag gtgaccagca ccaggccaaa gagcgtggtc
    12961 gccacaccga ccgcgagccg cgccgcggcc gccgcccgcg cccgtctact tgcggaggcc
    13021 atggtagctg gtctggtcgg cggtgatccc ggccgcgaag ccctcgaccc ggtcgctgag
    13081 cgcgatcacc tcgttctcct ggaacagcac gatgaacggc gaatccgcct gcacctcgcg
    13141 ttgcagctcc tggtacagcc gcgcgcggcg cgccggatcg cgctcgagca cggcggcatc
    13201 ggcgcgcgcg gtcagctccg ggatctccca gccgttgcgc caggccagcg tgcgctcggc
    13261 agcaccgtcg ccattgtcgg ggttgcgcgc aaaggtggac gcgttggtgt gcgggtctag
    13321 gtagtcgggt ccccactggc ccatgaacag ctcgtggcgg cgcgcgcggt agcgggtcag
    13381 cacctgcttg gcgctgccgg gcacgatctc gatcctgacc ccggcctcgg ccatggtcgc
    13441 ctggaccgcc tgggcgaggc gcaacgcgat cgggtccgag cgcacgtcga gccgcgcctc
    13501 gaagccgccg gcgagccccg cttcagagag aagtccgcgc gcccgctcgg gctcgaagtc
    13561 gaacggccgg tcgtcgagcg cgcccaggaa tccctgcggc aggaacgcct ggtgcactct
    13621 cgccgccggc ccgagcaggc gctcggcaat cccctggtag tccaccaggt agcgcagcgc
    13681 cttgcgcacc gcgggccggt cgagcggcgg gcgcttctgg ttgagcgcta ggtagaacag
    13741 gcgcgcgcgc ggcaccacca gcaggcggat gccgtcgatg ccgtcgaccg cggcaagctg
    13801 atcgggaccg agattgcgcg ccatgtcgac gtcgccgcgc tcgagcagca ggcgctgcgt
    13861 cgatggctcg cggatgtcgc ggatcgcgac ccgcttcagc gccggcggcc cttgccaata
    13921 gctggggttg gcgtcgagca gcaggtactc gcccggctgc cagcggcgta agctgaacgc
    13981 gcccgagccc gcagcgtgat ttctcagcca gccttcgccg agatcgccgt cggcgttggc
    14041 gagcaccgcc tcgcggtcga ccaccgaggc gatccccgcg ctcagcgcgt tcagcaccag
    14101 cgacggcgcg taggcgcgat cgagcgtgat cgtgaaccgc cggtcgtcga gcgcgcgcac
    14161 ccgttcgccc acattgtcgc cgctcaggcc gaactgggtc aggatgaagc tcggcgcctt
    14221 gttgagcgcg accgcgcgcc gcagcgagaa caccgcgtca tgcgcgtcca tcgcccggcc
    14281 ggaggcgaat ggcaggcgat cgcggacctc gaactcgaag ctcagactgt cggcgctcgc
    14341 tcgccaggac gagaccgcct cgcccagcgg cttggccgga tcgtccgggt tcggccggaa
    14401 caggcgcaaa tacaggttgg cggtgatctc ggcgcccgag aactcgaaca cctcggccgg
    14461 gtcgagcgtg atcaaatcgt ccagggactt ggccatcacc agcaccgagc gcggcgtcgc
    14521 cgccgccgca tcgcctgccg ccagcaccgc gaacatcgcc gcggccgtcg ccgccggacg
    14581 ccgaaagcgc cgaagtgcgc ggcgtgactc gatcatggcc gggccctata gtcgcgcccc
    14641 cgcgcgcccg cgcgtatttc gcccatcccc aaggcggcat ggccggtccg agcgtctccc
    14701 cctaccagat gatcgagcgg ctggtgtcct tcgacaccac cagccgcggc tcgaacctgg
    14761 acctgatcgg cttcgtcgcc gactacctcg ccggctacgg catcaaggcg acacgcctgc
    14821 tcgacgatgc cggcgccaag gccaacctgt tcgccaccat cgggcccgag cgtgacggcg
    14881 gcgtggtgct gtcggggcac accgacgtgg tgccggtcga cgaccaggac tgggactccg
    14941 acccgttcac ggtggtgcgc cgcggcggac gactgtacgg gcgcggcacc gcggacatga
    15001 aaagcttctc ggccgtcgtg cttgcgctgg tgcccgagct gatcgcgcgc ggcccgaagc
    15061 ggccggtgca cctcgccctg tcctacgacg aggaggtcgg ctgcctgggc gcgccgcggc
    15121 tggtcgagct gttgcgcgcc gcgtcgttcg cgcccgccgc ggtgatcgtc ggcgagccga
    15181 ccgggatgaa ggtggtcaac cggcacaagg gcgtgtaccg cttcaagacc tctgtcaccg
    15241 ggctcgaagc gcattccgcc cacaccgacc gcggggtcag cgccatcctg gtcgcgggcc
    15301 ggctgatcga gcgcctcgat cgcctggcac gtgccgcgcg cgcggccgcc gagcccggct
    15361 cgggcttcga tcccccttac cacacccttc acgtcggcct ggtcgaaggc ggcacggcgg
    15421 tcaacatcct tgcccgtcat tgcgccttcg actgggagct gcgcctgttg ccgggcggcg
    15481 acgcggcgcg cgacttcctc gacccgttcg aggcgttcgt gaccgccgag gtgctgcccg
    15541 agatgcacgc cgtctcctcg cgctgcgccg tggcgacggc gccggtggtc ggggtcgagg
    15601 gcttggcgcc cgtggccgac aacccggccg aggcgctggc gcgaacgctc gccggcgaca
    15661 acgccccggc cggggcgatc tcgttcggca ccgaggccgg cctgttccag cgcggcggca
    15721 cgcccactgt ggtctgcggc ccgggcagca tcctgcaggc gcacaaggcc aacgagtaca
    15781 tcgaggtcgc gcaggtcgag gcctgcatcg cgttcatgcg acggctgatc gcgtgggcgt
    15841 gcgactgact ccggcctcgc cctcgagcgc ccggcttgcg ccctcgatcg ccgtccgcag
    15901 gcggtccatg aacgcgccgc gctcgagccc cggcgggatc ggctcgagga attcgatgac
    15961 cgtgtgcgtc ggctgcttga cgaaccgccg ccggccccac gcgagtcccg agttgagcgc
    16021 caccggcacg accatcagat cgagatgctg atacagcgcc gccacgcccg gctggtaggc
    16081 gcgcgattct ccgggcgcga cccgggtgcc ctcgggaaag atgacgatcg gccggccgtc
    16141 ggccttggcg cgcgccgcct gcgcgagcag acgcttgagc gcgcgcggcc cggcgccgcg
    16201 gtcgaccacc gcgtagcgga gcttgaggaa cagccaaccc atcaccggca gcaggaccag
    16261 ctcgcgcttg atcacgaagc tcggatcggt gagcagcgcg gtcaatgcga ccgtctccaa
    16321 cgccgactgg tgcttgcagg cgacgatgca cgcgccggcg ggcagccggt cgagcccgcg
    16381 cacctcgata ccggcgccga tcaggccggt gaacaggcgc atcacggccc gcgcccaagt
    16441 ccgcacggcg ccgaccagcg ccgcgcgcgg catgatcaac gtcggcagca gcgccaccga
    16501 cagcagcgcc gatccgccga cgaaggcaag attgaacagg gccgagcgca gccattgact
    16561 cacggccgcg cctgcgcctc gttggcgaac accctcgcca ccaggtcggt cgccgccgcc
    16621 cgcgttctcg ccagcagcag cttgctgtac tcggtggcca gcaacccggc cgtcccgggc
    16681 gaccgccacc acgcgtcgag ccgcacggca ggcggaaaca ccgggtgcgc gaccaggtcg
    16741 acttcgggca tggcgcggcg gaactcgaac aggctgcgcg gcatgtggta ggcgccggtg
    16801 accagcctca ggctggcgaa gccttccgcc accatccatt cggcggtctc gcgcgcgttg
    16861 ccctccgtat cgccggcctc gtggccgagg gcgacgcggg cgctcagcgc ctcatcgccg
    16921 cgccgcgcca attgcagcaa ctcgtcgacc tcgacgccgc ggaacacgcc tgacaccagc
    16981 aacttctgcg cgcgcccctg cctgagcaac gccaagccct cgtcaagccg accgctgccg
    17041 ccggtcaata cgacgatcgc gtcggtggtc cggtccgggc gctcgaccgc cgtcggcaga
    17101 tcggcggcga agcggacgaa gccgaccgcc catgccgccg ccccggcaag cgcgaccacc
    17161 aacaccagac gccagcgacg ccgccggtcg agggttcgcg gcggcatcga cgggctacgg
    17221 catccgggcg agcgcgccga gcacggtcat ccgggcggtg accatggcga ggcccgcggt
    17281 gaccacgggc accgccgcca gcgcggccca ggcgcggacg ccgagcgccg cttcgggcag
    17341 cagcggcgcg tccaacctgc ccgcggcata gccgagggcg aacagcacgc cgaccgcggg
    17401 cagcacgccg cccagcccgc cgcgcaggcc cgtccacagc gcccgccact ggaactggcg
    17461 ggcgatgaac gaatcgtggg cgcccagcag gtacaacagc tccaccgcct cacgatgaac
    17521 cgcgagcccg gcccgggtcg cgaataccac cgagatcgcc gccacccccg cgaccaggac
    17581 caggatcatg gccgcgaaca gctgagccag gcgcatcagg tcgagcaggc gctcgaccca
    17641 tgccgtgccg gtatcgagca ccgcgctcgg cgccgcctcg gccagtcgcg cctcgagcgc
    17701 cggccagtcg accgccgcgc ccggcaccag gcggacgtcg atcaccaccg gcagcggcag
    17761 gtcctcgggc aggttgccgg acccgagcca gggctcgagc aaggccgcca ccgcctcggg
    17821 cgccagcgcc tcggcacttg ctatcccgag cgtccggctc accgtgtcga gggccgcggc
    17881 caccgcgtcg gctttggagc cggtgggaag ctgcaccgtg accacggcca tctccccgga
    17941 atgccagcgc gccgacatgt ccacggaacc gaccagcgcc gcaaccgcga gggcggcgaa
    18001 aaacgccttc agcccgatcg accaggacag gtagcggtca gcgccgccgc cgaccatgat
    18061 cgccgccttg cgccgcgcca tggcgactca ttcggccgcg ctgcgcgcgc cggcgggcgc
    18121 agcgagacgg ccatcctcga gcctcaggca gcgatgccca tagtccttga ccaggcgctc
    18181 gtcgtgggtg gcgatcacca ccgtggcgcc gagctcattg agcgcctcga acaggcgcag
    18241 caggcgctgc ccgttcgtcc ggtcgacatt gccggtcggc tcgtcggcga gcagcagcct
    18301 gggccggcgg atcaccgcgc gcgcgatcgc caggcgctgc ttctcaccgc ccgacaaggt
    18361 cggcggcagg gcatcgagct tgtcgccgag cccgacccat tcgagcagct cgcgcaccgg
    18421 cgccgcgatg tccggctcgc gcgcgccgga gatgcgcaac ggcagggcga cgttctggcg
    18481 cgcgctcagg tggtcgaaca ggcggaactc ctggaacacg atgccgatct tctggcgtaa
    18541 cccggccagc gtcgcgcggt cggcgccgtt cacgccggtg ccgagcaagg tcaccagccc
    18601 gcgcgacggc cgatggccga gatacagcag gcgcagcagc gacgacttgc cggcgccgct
    18661 cggaccggtc aggaacagga agcttcccgg ctccagggtg aacgagatgt cctgcaggac
    18721 ctccgggccg agcccgtagc gcatgcccac gttctcgaat cgaaccaagg cggcggcgcc
    18781 tccgttgccc acgattcgcc aaggtccaga ttcgcacacg cccgctggcc gcgtctagcg
    18841 gggcgatgct tgctttgtgc aagttcgcgc aatagttttg actggactcg agctgcggcc
    18901 ggttaagata ctatgatcct cacctgcccg tcctgcgaga ctcgcttctc ggtcgccgac
    18961 gacgcgctcg gcgctatggg ccgaaccgtg cgctgcggca cgtgccggca ccagtggttc
    19021 gagagcccgg gcggcgacgc gttcggcgcg ctggcgtacc agtccgacga caccgagccc
    19081 gaaaccgact tcgacagcct gttcgacacc gagcctagct acggctcgga tatccccgcg
    19141 tcgagtcagc acccggatga cgcgagtgag gcgccgctgg cggaggatga atgggactcc
    19201 gaggacgcgc cgcgaccggc cgcgcccgtc ttgaccgcgg cgccggcgtt ccgcccccgg
    19261 cagcgctcga gcggctggct tggctggacc gcggtcgcca gcgtcgtggt ggcgacgagt
    19321 gcggcggcag cgctgctcgc cgacctcgtg atcgaggtgt ggccaccgtc caagcggctt
    19381 tacgcgatgg ccggcctgcc attcgccggc cccggcgagg gccttgagat gcgcaacatc
    19441 accgcccgca tcgagcccgc cggcgacggc acgcggctgg tgatcgaggg caacatcgtc
    19501 aacgtctcgc gcgaggtcag gccggtgccg ctggtgcggg tggtgctgcg cgacgaccg
//
LOCUS       DASWHU010000282         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3129652, whole genome shotgun sequence.
ACCESSION   DASWHU010000282 DASWHU010000000
VERSION     DASWHU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129652"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGB88_10475"
     CDS             <1..366
                     /locus_tag="VGB88_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9647914.1"
                     /translation="RDYYVMNIIAQAFDDAQIDAIGAWFEDRPWISTPVPHDAVKASD
                     GKVLADGLCATCHGADGRGTGAGPRIAGQPAAYLSLALHAYKDGARNSASARVAKTLL
                     DDLSDEQIEAMSNYYAGLR"
     gene            383..1675
                     /locus_tag="VGB88_10480"
     CDS             383..1675
                     /locus_tag="VGB88_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEX9647915.1"
                     /translation="MTLTRRRFIAHGTAGLGALALGHLPLPALAQGEIMPASGKRVVV
                     VGGGWGGATAAKYIRMQDPGIEVVLVETGEVFRSCPISNWVIGGIRTMDDITVGYDGL
                     VAHHGVKLMRDTVTGIDTAAKTVSVESGGFLRYDRLVLSPGITLRRDAIAGLEAARER
                     FPAAWKAGPETQLLRDRLQAMAAGGTVALSVPPAPFRCPPGPYERVSLIADYLKRDKP
                     GSKLVVFDANQNIISKGALFQAAWDELYAGIIDYRPDHAVTRVDGAIGTLYTDFDEVT
                     AEVANVIPPQQANRLVHDAGLVPEGRQWAPVKPWDFTSTEADAVHVIGDATDGSVVGA
                     VPKSGFVANSMGKVAAAAAVALLNGREAPRPSLANTCYSLVSAEEGISVTAIYDYDDA
                     TGKIVAVEGASGLSPARTALIKRNAEDWAQAIWRDMLA"
     gene            complement(1685..>2628)
                     /locus_tag="VGB88_10485"
     CDS             complement(1685..>2628)
                     /locus_tag="VGB88_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9647916.1"
                     /translation="TLDALAAQGTGLDVVVVDDQSLDGTGEVARARAPVRVVAGAGLP
                     AGWTGKLWALEQGWRRIDRPLVLLLDADIALAPGMIAALRDKLRGDGLALVSVMASLR
                     MASGWERLLIPAFIYFFKLLYPFRLSNGTSRLVAAAAGGCVLVEAAMVERIGGFAALR
                     CALIDDCALARAVKRAGGRTWIGLSRGAVSRRAYDRLGPIWDMVARTAYAQLGNSPLW
                     LGACTAVMALAFWAPLAGALAGRGLLSAISAAGLAAMLASYQPVLAYYRRSPLWAPAL
                     PLIGTLYLAMTWASAVRHWRGAGAHWKGRRYAAGIAR"
BASE COUNT          402 a          974 c          949 g          303 t
ORIGIN      
        1 cgcgactact acgtcatgaa catcatcgcc caggcgttcg acgacgccca gatcgacgcc
       61 atcggcgcct ggttcgagga ccggccgtgg atcagcaccc cggtgcccca cgacgcggtc
      121 aaggcgagcg acggcaaggt cctcgccgac ggcctgtgcg cgacctgcca cggcgccgac
      181 ggccgcggca cgggcgccgg gccgcgcatc gccggccagc ccgcggcgta cctgtcgctc
      241 gctctccacg cctacaagga cggcgcgcgc aacagcgcca gcgcgcgcgt cgccaagacc
      301 ttgctcgacg acctgtcgga cgagcagatc gaggcgatgt ccaactacta cgccgggctg
      361 cgctgagccg agggagggtg ccatgacact gacacgcaga cgtttcatcg cccacggcac
      421 cgccgggctc ggggcgctgg cgctcggcca cctgccgctg ccggcgctcg cccaaggcga
      481 gatcatgccc gcctcgggca agcgggtggt ggtggtcggc ggcggctggg gtggcgccac
      541 cgccgccaag tacatccgca tgcaggaccc ggggatcgag gtggtgctgg tcgagacggg
      601 cgaggtgttc cgctcctgcc cgatcagcaa ctgggtgatc ggcggcatcc gcaccatgga
      661 cgacatcaca gtcggctatg acggcttggt cgcccatcac ggggtcaagc tgatgcgcga
      721 tacggtcacc ggcatcgata ccgccgccaa gacggtgtcg gtcgagagcg gcggcttcct
      781 gcgctacgac cggctggtgc tgtcgccggg catcacgctc cgccgcgacg cgatcgcggg
      841 cctggaggcg gcgcgcgagc gcttcccggc ggcgtggaag gcggggccgg agacccagct
      901 cctgcgcgac cgcctgcagg cgatggccgc cggcggcacg gtggcgctga gcgtgccgcc
      961 ggcgccgttc cgctgcccgc cgggaccgta cgagcgcgtc tcgctgatcg ccgactacct
     1021 caagcgcgac aagccgggct ccaagctggt ggtgttcgac gccaaccaga acatcatctc
     1081 caagggcgcg ctgttccagg ccgcctggga cgagctgtac gccggcatca tcgactaccg
     1141 cccggaccac gccgtcaccc gggtcgacgg cgccatcggc accctctaca ccgacttcga
     1201 cgaggtcacc gccgaggtcg ccaacgtcat cccgccgcag caggcgaacc ggctggtcca
     1261 cgacgccggg ctggtgcccg aggggcggca atgggcgccg gtcaagccgt gggacttcac
     1321 ctcgaccgag gccgatgccg tccacgtcat cggcgatgcg accgacggca gcgtggtcgg
     1381 cgcggtgccc aagtcgggct tcgtcgccaa ctcgatgggc aaggttgccg cggccgcggc
     1441 ggtagcgctg ctcaacgggc gcgaggcgcc gcgcccgagc ctcgccaaca cctgctacag
     1501 cctggtcagc gccgaggagg gcatctcggt gaccgcgatc tacgactacg acgacgccac
     1561 gggcaagatc gtcgcggtcg aaggcgcgag cggcctgtcg ccggcgcgca ccgcgctgat
     1621 caagcgcaac gccgaggact gggcgcaggc gatctggcgc gacatgctcg cctgagccgg
     1681 cgcgtcagcg ggcgatgccg gcggcgtagc gccggccctt ccagtgcgcg ccggcgccgc
     1741 gccagtggcg gaccgccgag gcccaggtca tggccagata gagcgtgccg atcagcggca
     1801 gcgccggtgc ccacagcggc gagcgccggt agtaggcgag taccggctgg tagctcgcca
     1861 gcatcgccgc gagcccggcg gcgctgatgg cgctgagcag gccgcggccg gcgagcgcgc
     1921 cggcgagcgg cgcccagaag gcgagcgcca tcaccgccgt gcaggcgccg agccacagcg
     1981 gcgaattgcc gagctgggcg tacgccgtgc gcgcgaccat gtcccagatc ggcccgagcc
     2041 ggtcataggc gcgtcggctg accgcgccgc gcgacaggcc gatccaggtc cgcccgccgg
     2101 cccgcttgac cgcgcgggcg agcgcgcagt cgtcgatcaa ggcgcagcgc agcgcggcga
     2161 agccgccgat ccgctcgacc atcgccgcct cgacgagcac gcagccgccg gccgccgccg
     2221 cgaccaggcg cgaggtcccg ttcgacagcc ggaacgggta cagcagcttg aagaaataga
     2281 tgaaggccgg gatcagcagc cgctcccagc ccgaggccat gcgcaacgac gccatcaccg
     2341 agaccagcgc caggccgtcg ccgcgcaact tgtcgcgcag cgcggcgatc atgccgggcg
     2401 cgagcgcgat gtcggcgtcg aggagcagga cgagcggccg gtcgatgcgc cgccacccct
     2461 gctcgagcgc ccacagcttg ccggtccagc cggcgggcag gcccgcgccc gccaccaccc
     2521 gcaccggcgc gcgggcgcgc gcgacctcgc cggtgccgtc cagcgactgg tcgtcgacca
     2581 ccaccacgtc gaggccggtg ccctgggcgg cgagcgcatc gagggtcg
//
LOCUS       DASWHU010000283         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_151299, whole genome shotgun sequence.
ACCESSION   DASWHU010000283 DASWHU010000000
VERSION     DASWHU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151299"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="pepN"
                     /locus_tag="VGB88_10490"
     CDS             complement(<1..351)
                     /gene="pepN"
                     /locus_tag="VGB88_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX9647917.1"
                     /translation="MTEPRRQPVLLADYRPPAYRIARVELDIDLDEAETRVRSRLEVA
                     RASKDAVPLVLDGEDLALDSVAIDGRRLAPSAYQVDDTTLTLPALDRAVVEIETACRP
                     RDNTRLEGLYVSNGT"
     gene            complement(370..1056)
                     /locus_tag="VGB88_10495"
     CDS             complement(370..1056)
                     /locus_tag="VGB88_10495"
                     /inference="COORDINATES: protein motif:HMM:NF022599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HEX9647918.1"
                     /translation="MIGGLAAWPAAAALGAGARAGAAIAPADLLTFFDTLALQRSVGR
                     GESRPGIVRKWTEPVAVRVRGVAAAPYQDSIRAMLAEVSALAGLDFALATPDGATANL
                     ITLYYLSRREIGRLYPRARAVCFTQARGQGGHIHTGEVRIGADFEDCLHHEFMHALGF
                     DNHWEGRRFGMFAPTALADRFAPERTRDFSDWDRLAIRLLYDRRLDAGLARAPALEVA
                     RALVPELLTA"
     gene            complement(1266..2291)
                     /locus_tag="VGB88_10500"
     CDS             complement(1266..2291)
                     /locus_tag="VGB88_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9647919.1"
                     /translation="MTVEAPVSKLKPADFASDQELRWCPGCGDYAILKAIQKTLADVA
                     AKRESTVFISGIGCSSRLPYYMSTYGFHTIHGRAPAFATGIKLANPELDVWLITGDGD
                     GLSIGGNHMMHVLRRNVDVQILLFNNEIYGLTKGQYSPTSRIGTRSPSTPMGSVDAPV
                     SPCMLALGSGARFVARAVDTAIKHLSEVLARAYRHKGASFVEIYQNCIVYNDKVFADF
                     TEREGAPDHQLVVAHGKPLMFGADRQKGLRFRPGTMTLEVVTVGEGGVTEADVLVHDE
                     TNRSLAMLLAELSGPEFPVALGVLYCEPRPTYERAVWAQIEDASKAGTPRWNDILRRG
                     YTWTVEP"
     gene            complement(2307..>4082)
                     /locus_tag="VGB88_10505"
     CDS             complement(2307..>4082)
                     /locus_tag="VGB88_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9647920.1"
                     /translation="MQLTGSQFTLATAIQGNDLATFPDFPAEIRAPAGTTFGVSAFQI
                     NFSAHEVLTAGDAPDVLVAMNPAALKVNLGDVPAGGTLVVDTGSFTERNLHKAGFESN
                     PLADHSLHGYHLIAVDMSQNTLNAVAESGLGQKEGLRCKNMWALGLIMWMFSRDREPT
                     IKWLRNRFKDRPDIAEANVAALNAGHAFGETAELPDGVSRRIIPRAQIEPGLYRAVSG
                     SEALAWGLAVGADLAGLKLVFGSYPITPASPLLHILAGLKSMGVVTFQAEDEIAAICS
                     AIGASYAGALGVTSSSGPGIALKTEAMGLAVVAELPLVVVDSQRAGPSTGMPTKTEQS
                     DLLQAVWGRNGDTPIPVVAAYSPASCFTLALEAVRVATRFMTPVIVLSDGYLANAAEP
                     WRLPDIDALARFPVRFRTDPGGFHPYLRDPDTLARPWAIPGTPGLEHRIGGIEKDYES
                     GNISYDPANHQRMTMVRHDKVAGIANFVAPQEVTCGEDSGALAVVGWGSTFGAIDQAV
                     RRARARGKPVSHIHLTWLNPFARGLGMLLSRFDKVLVPEMNTGQLVRMLRAEFLVPAR
                     GLNKVSGQPFKVAEVLAAIDTALEA"
BASE COUNT          600 a         1385 c         1501 g          596 t
ORIGIN      
        1 ggtgccgttg gagacgtaca gcccttcgag ccgggtgttg tcgcgcggcc ggcaggcggt
       61 ctcgatctcg accaccgccc gatccagcgc cggcaaagtg agggtcgtgt cgtcgacctg
      121 gtaggcggag ggcgcgaggc ggcggccgtc gatcgcgacc gaatcgagcg cgaggtcctc
      181 gccgtcgagc accagcggca ccgcgtcttt ggacgcgcgc gcgacctcga gccggctccg
      241 cacgcgggtc tcggcctcgt cgaggtcgat gtcgagctcg acgcgcgcga tgcggtaggc
      301 gggcgggcga tagtcggcga gcagcaccgg ctgccggcgg ggctcggtca tggcgttacg
      361 cccagggcct cacgcggtca gcagctcggg gacgagcgcg cgcgcgacct caagcgcggg
      421 cgcgcgcgcc aggcccgcgt ccagcctccg gtcgtagagc aggcggatcg cgaggcggtc
      481 ccagtccgag aagtcgcgcg tgcgctcggg cgcgaatcgg tcggccagcg ccgtgggcgc
      541 gaacatgccg aagcgccggc cctcccaatg gttgtcgaag ccgagcgcgt gcatgaactc
      601 gtggtgcagg cagtcctcga agtcggcgcc gatgcgcacc tcgccggtgt ggatgtggcc
      661 gccctgcccg cgcgcctggg tgaagcacac ggcacgcgcg cgcgggtaca gccggccgat
      721 ctcgcgccgc gacaggtagt agagggtgat caggttggca gtggcgccgt ccggcgtggc
      781 gagcgcgaag tcgagcccgg cgagcgcgct cacctcggcg agcatagcgc ggatcgagtc
      841 ctggtacgga gcggcggcaa ccccgcgcac gcgcaccgcc accggctcgg tccacttgcg
      901 cacgatgccc ggccgggatt cgccccggcc gacgctccgc tggagcgcga gggtgtcgaa
      961 gaaggtgagc aagtccgcgg gcgcgattgc cgccccggcc cgcgcgcccg cgccgagcgc
     1021 cgccgcggcc ggccacgcgg caagcccgcc gatcaagctc ctgcgcgtga tcagtctgcg
     1081 cgggcggcct tcgccaagcg gcgccgcgct tccgaggcag tacccatgat cacgagcgac
     1141 attctgcgct gccgccatgc aacgcatctt gtctcatccg gactccagat gctacccgtc
     1201 cgctggagtc tgggatgccc gcctcacgga attgggagcg aaatacgccc ggatcggccg
     1261 cctgcttacg gctccactgt ccaggtgtag ccgcggcgga ggatgtcgtt ccagcgcggc
     1321 gtgccggcct tggacgcgtc ctcgatctgc gcccacaccg cgcgctcgta agtggggcgc
     1381 ggctcgcagt agagcacgcc gagcgcgacc gggaactcgg gccccgacag ctcggcgagc
     1441 agcatggcga gcgagcggtt ggtctcgtcg tgaaccagca cgtccgcctc ggtaacgccg
     1501 ccctcgccga cggtcaccac ctcaagcgtc atcgtgcccg ggcggaagcg caggcccttc
     1561 tgccggtcgg cgccgaacat cagcggcttg ccgtgggcga ccacgagctg gtggtcgggg
     1621 gcgccctcgc gctcggtgaa gtcggcgaac accttgtcgt tgtagacgat gcagttctgg
     1681 tagatctcga cgaacgaggc gcccttgtgc cggtaggcgc gcgccagcac ctcgctcaga
     1741 tgcttgatcg cggtgtcgac cgcgcgggcg acgaagcgcg cgcccgagcc gagcgccagc
     1801 atgcacggcg agaccggcgc gtccaccgag cccatcggtg tcgagggcga gcgcgtgccg
     1861 atgcgcgagg tgggcgagta ctggcccttg gtcaggccgt agatctcgtt gttgaacagc
     1921 aggatctgga cgtcgacgtt gcgcctgagc acgtgcatca tgtggttgcc gccgatcgac
     1981 aggccgtcgc cgtcgccggt gatcagccac acgtcgagct ccgggttggc cagcttgatg
     2041 ccggttgcga acgccggcgc gcggccgtgg atggtgtgga agccgtaggt cgacatgtaa
     2101 tagggcaggc gcgacgagca gccgatgccg gagatgaaca ccgtgctctc gcgcttggcg
     2161 gcgacgtcgg cgagggtctt ctggatcgcc ttcaggatcg cgtagtcgcc gcagccgggg
     2221 caccagcgca gctcctggtc ggaggcgaag tcggccggct tgagcttgga gacgggcgct
     2281 tcaacggtca tctcaggcct cccaactcag gcttccaagg cggtgtcgat ggcggcgagg
     2341 acctccgcca ccttgaacgg ctggccgctg accttgttga gcccgcgcgc cggcaccagg
     2401 aactcggcgc gcaacatcct gaccagctgg ccggtgttca tctcgggcac cagcaccttg
     2461 tcgaaccggg acaggagcat accgaggccg cgcgcgaacg ggttgagcca ggtgagatgg
     2521 atgtgcgaga ccggcttgcc gcgggcgcgc gcgcgccgca ctgcctggtc gatggcgccg
     2581 aaggtcgagc cccagccgac taccgccagc gcacccgagt cctcgccgca ggtgacctcc
     2641 tgcggcgcga cgaagttggc gatgccggcc accttgtcgt gccggaccat ggtcatgcgc
     2701 tggtggttgg ccgggtcgta agagatgttg ccggactcgt agtccttctc gatgccgccg
     2761 atccggtgct cgagccccgg cgtgcccggg atcgcccacg gccgggcgag ggtgtcgggg
     2821 tcgcgcagat aggggtggaa gccgcccggg tcggtgcgga agcggaccgg gaagcgcgcc
     2881 agcgcgtcga tgtcgggcag ccgccagggc tcggcggcat tggccagata accgtcgctg
     2941 agcacgatca ccggggtcat gaaccgggtc gcgacgcgca ccgcctcgag cgcgagcgtg
     3001 aagcagctcg ccggcgagta ggcggcgacc accgggatgg gcgtgtcgcc gttgcgcccc
     3061 cagaccgcct gcaacaggtc cgactgctcg gtcttggtcg gcatgccggt ggacggcccg
     3121 gcgcgctggg aatcgaccac caccagcggc agctcggcga ccacggcaag gcccatcgcc
     3181 tcggtcttga gggcgatgcc ggggcccgag ctcgaggtca ccccgagcgc gccggcatag
     3241 gaggcgccga tcgccgagca gatcgccgcg atctcatcct cggcctggaa ggtgaccacg
     3301 cccatcgact tgagcccggc caggatgtgc agcagcggcg aggccggggt gatcgggtac
     3361 gagccgaaca ccagcttgag gccggcgagg tcggcgccga ccgcgagccc ccaggcgagc
     3421 gcctcgctgc cgctgaccgc gcggtacagc ccgggctcga tctgggccct cgggatgatc
     3481 ctgcgcgaca ccccgtccgg cagctccgcg gtctcgccga aggcgtggcc ggcgttcagc
     3541 gccgcgacgt tggcctcggc gatatccggc cgatccttga atcggttgcg cagccatttg
     3601 atggtcggct cgcgatcgcg gctgaacatc cacatgatca ggcccagcgc ccacatgttc
     3661 ttgcagcgca ggccttcctt ctggccgagc ccggattccg ccaccgcatt gagcgtgttc
     3721 tgcgacatgt cgacggcgat caggtggtag ccgtgcagcg agtggtccgc gagcgggttg
     3781 gactcgaagc cggccttgtg caggttgcgc tcggtgaacg agccggtgtc gaccaccagc
     3841 gtgccgcccg ccggcacgtc gccgaggttg accttgagcg ccgccgggtt catcgccacc
     3901 agcacgtccg gcgcatcgcc cgcggtcagc acctcgtgcg ccgagaagtt gatctggaac
     3961 gcggacacgc cgaaggtggt gccggcgggg gcgcgaatct cggccgggaa gtcggggaag
     4021 gtggcgaggt cgttgccctg gatggcggtc gccagcgtga actggctgcc ggtgagctgc
     4081 at
//
LOCUS       DASWHU010000284         8704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_896754, whole genome shotgun sequence.
ACCESSION   DASWHU010000284 DASWHU010000000
VERSION     DASWHU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896754"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1502)
                     /locus_tag="VGB88_10510"
     CDS             complement(6..1502)
                     /locus_tag="VGB88_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX9647921.1"
                     /translation="MRFAVGGFQHETNTFAPVKTGFEDFVRADGWPALSRGEALFDSV
                     EGTNLPIAGFVDAALAGGHALAPLMWCAAVPAAHVEECAYERIAAMILDDLARAGPID
                     GVYLDLHGAMVAEHLEDGEGELLARVRAAVGPAVPVVASLDLHANVTLRMVELADALV
                     AYRTYPHVDMARTGARAAALLARMAAGARPAKAWRKLDFLIPLVWQCTDLEPARALYR
                     RIEALEAEDEGLWSLSLCTGFAPADIRDAGPAVLAYGDDRAGADRAAEALAQALAGAE
                     PAFAGRIYVADEAVAFALAQDRRPVVIADTQDNAGAGGNGDTVGMLRALVDAGADEAV
                     FAIVNDAAAAAAAHAAGEGAEIEIALGAASAWQGERPFQGRFAVRALGSGRFTATGPM
                     FGGARMELGPMAVLGHRGIEVVVASRKMQAADQAMLRHLGIAPETRRILVLKSSVHFR
                     ADFAPIAAAVIVAASPGPNPVDHARLAYRRLRPGVRLMPLGEAYPGAA"
     gene            complement(1543..3003)
                     /locus_tag="VGB88_10515"
     CDS             complement(1543..3003)
                     /locus_tag="VGB88_10515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9647922.1"
                     /translation="MLKARLLEFHRWLGALALFAGLLAGTGPAPAAGLPALLSDADEA
                     RIGAEQHQEILAEFGGAYEDPKLAAYVDSIGQFLALTSERPNLKFTFTVLDSPIVNAF
                     ATPGGYVYVSRGLLALAGDEAEVAGVIAHEIGHVAARHGVQRIEGGMLANLGLGLLGL
                     LTKNEQIAGLAQYGALAVLQGYSREQEYEADLLGVRYLSRAGFDPEAMASFLNKMKAS
                     SELDAIESGGSDGERLDLMSTHPRTAERVHRAIAAAGARKVSNPIVGRDIYLTKIDGI
                     LYGDDPAQGLVRDRRFIHPALRFAFDAPADFALLNGETQVIGRGPQGALMVFDGAARD
                     DRRAMARYIGEVWLTKAAPAAEPIRIDGRDAATAAFRGSVQGQPTDVRAVAIAFDSRT
                     VYRFLFLTPPAVTDQFDESFRRTAYSFRSISAAEAQSARPRRMVLHRVADGETSAQVA
                     RLMAVDKNPLERFLILNGLYPNQPLVPGSVVKVVVE"
     gene            3377..4261
                     /locus_tag="VGB88_10520"
     CDS             3377..4261
                     /locus_tag="VGB88_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9647923.1"
                     /translation="MNLTVRFAAVAAGLSLAACYTSDHIDVANMTPSGSAFQAELHKA
                     YSERSQLEYDEGDWSDGYRASDKARRAAGGENVMPELVWDWDLPEETVDEFHEARDTL
                     GYWMRVGAAERVPALMATAQVQYDCWLEEQEEGHQADDIASCRNGFYNAIAEIDKAMV
                     WEAVPPAPMPMPGPTPRPMAEEPGGMPRFYTIFFDWDKSDINPVAQRVVTAIVDDWGA
                     ASDSLQLVGHADRSGGDDYNQRLSERRVASVTAALGAQGFRESRLSGYGVGETDPAVP
                     TPDGVREPRNRRVVVTLQ"
     gene            4495..5163
                     /locus_tag="VGB88_10525"
     CDS             4495..5163
                     /locus_tag="VGB88_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647924.1"
                     /translation="MVARLSGAVVVAVILSGGALAGGQAVLPFEQSAAYKVDSRSVLH
                     FDDYAGKITDQRIKRLLRSGDILGDRVLLQNDVAVYFPDGHIVSAKAEFHTYRPVAGL
                     FPWNVYSAVQVEPNPNYQYRSEFDALLGPACAETVYSKGSKRRPGESYARLRINRQNV
                     LSLELDSPGLYDYLTGEPSRSYTVTSALDPYYLGDAQVVQQLMNTCIEYQRTAIEQAK
                     AGDR"
     gene            5370..6173
                     /locus_tag="VGB88_10530"
     CDS             5370..6173
                     /locus_tag="VGB88_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9647925.1"
                     /translation="MYRLILVALLAATTLIGCVPLRQGNTLGYDFAVTVDRAELPDQS
                     CKVDLTLHNNLDSDFSNFEYGLNFFDSLGRWAAGPVVQGDFIATGGVSKQDFIFPVAC
                     DRLASATVAKQLCLDIIGMAGRRLCYDIGQIVINDVVRAEVLGPPEGVEAPPAGKPRF
                     YTLFFDWDKSDINDVAQLVIDAIVADWTGLPDSLQLVGHADRSGPVDYNQKLSERRVA
                     SVAQVLIGQGIEAERVSGYGLGETDPLVPTPDGVREPRNRRVVVTLETR"
     gene            complement(6245..7963)
                     /gene="ilvD"
                     /locus_tag="VGB88_10535"
     CDS             complement(6245..7963)
                     /gene="ilvD"
                     /locus_tag="VGB88_10535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9647926.1"
                     /translation="MSRIDKSKLPSRHATEGPERAPHRSFYYAMGLSDKEVHQPFVGV
                     ATTWNETAPCNITLRRQAQAAKRGVVFAGGTPREFTTITVTDGIAMGHQGMKASLVSR
                     EAIADTVELTMRGHAYDALVGIAGCDKSLPGMMMAMLRLNVSSVFIYGGSILPGRFQG
                     RDVTVQDVYEAVGMHSAGKMTDAELFQLEHVACPSGGSCGGQFTANTMACVSEALGLA
                     LPASTAVPAPYESRDEYADDAGRQVMALLEAGIRPRDIVTREALENAATVVAASGGST
                     NAALHLPALANEAGIEFSLLDVAEIFKRTPYLADLKPAGRFVAKDMYEAGGVPMLMKA
                     LLDGGFLNGGCMTVTGRTVAENLAEVRFRTDQEVVRPADNPLSPTGGVVGLQGNLAPE
                     GAIVKVAGMKTLTFRGIARVFDREEDAFAAVERRDYKEGEVLVIRYEGPKGGPGMREM
                     LATTAAIYGQGMGDKVALITDGRFSGATRGFCVGHVGPEAAVGGPIGLLKDGDVVVID
                     AAAGQLDVELSDRELAERRKSWAPREHDYRSGELWKYTQLVGSALNGAVTHPGARAEI
                     KCYADI"
     gene            8125..8520
                     /locus_tag="VGB88_10540"
     CDS             8125..8520
                     /locus_tag="VGB88_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647927.1"
                     /translation="MPVLRAFVLVLLALGAGLPRPAGADLVKPLLCPQAEILCLQKLG
                     YNWQKCGTLTGPGGGAQEFPLCPAADGYACVPCWFGSLAETAAQCRARFGPDCGHFVE
                     HDARWRGEWGAFAWPENSSLFLLFGAGPH"
     gene            complement(8534..8692)
                     /locus_tag="VGB88_10545"
     CDS             complement(8534..8692)
                     /locus_tag="VGB88_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647928.1"
                     /translation="MSEIAKLDTKLSNEQFLARAPEDVIAEQRERRAEADLSRRKLDQ
                     AVARLSAV"
BASE COUNT         1258 a         3056 c         3072 g         1318 t
ORIGIN      
        1 gcgcgtcagg cggcgccggg ataggcctcc ccgagcggca tcagcctgac ccccgggcgc
       61 agccggcggt aggcgagccg ggcgtggtcg accgggttcg gcccgggcga tgcggcgacg
      121 atcactgcgg ccgcgatcgg cgcgaagtcg gcgcggaaat ggaccgagct cttgagcacc
      181 aggatgcgcc gcgtctcggg cgcgatgccg aggtggcgca gcatcgcctg gtcggcggcc
      241 tgcatcttcc tcgacgccac caccacctcg atgccgcgat ggccgagcac cgccatcggc
      301 ccgagctcca tgcgcgcgcc gccgaacatc ggcccggtgg cggtgaaccg gccgctgccg
      361 agcgcgcgca ccgcgaaccg gccctgaaac ggccgctcgc cctgccacgc cgaggcggcg
      421 ccgagggcga tctcgatctc cgccccctcg ccggccgcgt gcgccgccgc cgccgcggcg
      481 gcgtcgttga cgatcgcgaa cacggcctcg tcggcgccgg cgtcaaccag cgcacgcagc
      541 atgccgacgg tgtcgccatt gccgccggcg ccggcattgt cctgggtgtc ggcgatcacg
      601 accggccggc ggtcctgggc gagcgcgaag gccaccgcct cgtccgcgac gtagatgcgg
      661 ccggcgaacg cgggctcggc gccggcgagc gcctgcgcca gcgcctccgc cgcccggtcc
      721 gcgccggccc ggtcgtcgcc gtaggcgagc accgccgggc ccgcgtcgcg gatgtcggcc
      781 ggggcaaagc cggtgcacag cgacagcgac cacaggccct catcctcggc ctccagcgcc
      841 tcgatgcggc ggtagagtgc gcgcgcgggc tcgaggtcgg tgcactgcca gaccagcggg
      901 atcaggaagt cgagcttgcg ccacgccttg gccgggcggg cgccggcggc catgcgcgcc
      961 agcagcgcgg ccgcgcgcgc gccggtgcgc gccatgtcga catgcggata ggtgcgataa
     1021 gcgaccagcg catcggcgag ctcgaccatg cgcagggtga cgttggcatg cagatcgagg
     1081 ctcgccacca ccggcaccgc gggcccgacc gccgcacgca cgcgcgcgag cagctcgccc
     1141 tcgccgtcct cgaggtgctc ggcgaccatg gcgccgtgca ggtccagata gactccgtcg
     1201 atgggcccgg cgcgggcgag gtcgtcgagg atcatcgccg cgattcgctc gtacgcgcac
     1261 tcctcgacat gcgccgcggg caccgccgcg caccacatca gcggcgccag cgcgtggccg
     1321 ccggcgagcg ccgcgtcgac gaagcccgcg atcggcaggt tggtgccctc gaccgaatcg
     1381 aacagcgcct cgccccgcga cagcgccggc cagccgtcgg cgcggacgaa atcctcgaac
     1441 ccggtcttga ccggcgcgaa cgtgttggtc tcgtgctgga agccgccgac cgcgaacctc
     1501 atcgatgcca ccccggcgcc cgcgccgcgt cgccgcgcgg cgctactcca ccaccacctt
     1561 gaccaccgag cccgggacca gcggctggtt tggatacagg ccgttgagga tcaggaaccg
     1621 ctcgagcggg ttcttgtcga ccgccatcag gcgcgccacc tgcgccgagg tctcgccgtc
     1681 ggcgacccgg tgcagcacca tccgccgggg ccgcgccgac tgcgcctcgg cagcgctgat
     1741 cgagcggaag ctgtaggcgg tgcgccggaa cgactcgtcg aactggtcgg tgacggcggg
     1801 cggggtcagg aacaggaacc ggtagacggt gcgcgaatcg aaggcgatcg ccaccgcgcg
     1861 cacgtcggtg ggctggccct ggacgctgcc gcggaaggcc gcggtcgccg cgtcccggcc
     1921 gtcgatccgg atcggctcgg cggccggcgc cgccttggtc agccagacct cgccgatgta
     1981 gcgggccatc gctcggcggt cgtcgcgcgc cgcgccgtcg aacaccatca gcgccccttg
     2041 cgggccgcgc ccgatcacct gcgtctcgcc gttgagcagg gcgaagtcgg ctggcgcgtc
     2101 gaacgcgaat cgcaacgccg gatggatgaa tcgccggtcc cgcaccaggc cctgggccgg
     2161 atcgtcgccg tagaggatgc cgtcgatctt ggtcaggtag atgtcgcgcc cgacgatcgg
     2221 gttgctcacc ttgcgcgccc ccgccgcggc gatcgcccgg tgcacccgct cggcggtgcg
     2281 cggatgggtc gacatcaggt cgagccgctc gccgtcgctg ccgccggact cgatcgcgtc
     2341 gagctcgctg ctcgctttca tcttgttgag gaacgaggcc atcgcctcgg gatcgaagcc
     2401 ggcgcgcgac aggtagcgga ctcccagcag atcggcctcg tactcctgct cgcgcgagta
     2461 gccctgcagc accgccaggg cgccgtactg ggcgaggccg gcgatctgct cgttcttggt
     2521 caagaggccg agcaggccca gcccgaggtt ggcgagcatg ccgccctcga tccgctgcac
     2581 gccgtggcgc gcggcgacgt gaccgatctc gtgcgcgatc acgccggcga cctcggcctc
     2641 gtcgccggcc agcgccagca gtcccctgga cacgtagaca tagccgcccg gggtggcgaa
     2701 ggcgttgacg atcgggctat cgagcacggt gaaggtgaac ttgaggttcg gccgctccga
     2761 ggtcagcgcc aggaactggc cgatgctgtc gacataagcg gcgagcttcg ggtcctcgta
     2821 agcgccgccg aactcggcca ggatctcctg gtgctgctcg gcgccgatgc gcgcctcgtc
     2881 ggcgtcggac agcagcgccg gcaggccggc cgcgggcgcc ggccccgttc cggcgagcag
     2941 cccggcgaac agggcgagcg caccaagcca gcggtgaaat tccagcagtc gtgccttcaa
     3001 caagtcttgc tcctttgccg gcctgcggtc atgatcgcgc cgattcccag tctcggcctc
     3061 gcccgagcaa atatgcatcc gcgcggcccg gttccagccg gtcgaggcaa acgcgcgagg
     3121 tcgtgccgca gttgcgaaat agtcacactg tgacaacttt cggctaaatc gtttgtcaga
     3181 atggcaaaat tcccctatca cttgatccgg gcggctgttc agggggaacg ttaccatcta
     3241 acttgttcaa ggcttaggtc cgaggctgtc ggaggcggcg atcagacgca ttcaacggtg
     3301 gccgcacatc aacgcggggt gcctccgagc gcggagggat gataccggca acggggaaga
     3361 gggttagagg actccgatga acctgactgt tcgctttgcc gccgtggcgg cgggcttgtc
     3421 tctggcggcc tgctacacga gcgatcatat cgacgtggcc aacatgaccc cgtcggggtc
     3481 cgccttccaa gccgaactgc acaaggcata ttcggagcgg tcgcagctcg agtacgacga
     3541 gggcgactgg tcggacggct atcgcgcctc cgacaaggcg cgccgcgccg ccgggggcga
     3601 gaacgtgatg ccggagctgg tctgggattg ggacctgccg gaggagaccg tcgacgagtt
     3661 ccacgaagcg cgcgacacgc tcggctactg gatgcgggtg ggcgccgccg agcgcgtgcc
     3721 ggcgctgatg gccactgctc aggtgcagta cgactgctgg ctcgaggagc aggaggaggg
     3781 tcatcaggcc gacgacatcg cgagctgccg gaacggcttc tacaacgcga tcgccgagat
     3841 cgacaaggcg atggtctggg aggcggttcc gccggcgccg atgccgatgc ccgggccgac
     3901 gccgcggccg atggcggaag agccgggcgg gatgccgcgc ttctacacca tcttcttcga
     3961 ctgggataag tccgacatca acccggtcgc ccagcgcgtg gtcaccgcga tcgtcgacga
     4021 ctggggtgcg gcaagcgatt cgctgcagct ggtcgggcac gccgaccggt cgggtggcga
     4081 cgactacaat cagcggctgt ccgaacggcg cgtcgcctcg gtgacggcgg cgttgggcgc
     4141 acagggcttc cgcgaatcgc ggctgtccgg ctacggcgtc ggcgagaccg atcccgccgt
     4201 gccgaccccg gacggtgtgc gcgagccgcg caaccggcgc gttgtcgtga ccttgcagta
     4261 acgcgacgtg acttgacggc cagcgacaag gcccgggagg cgagcggata ccgccttccg
     4321 ggcctttcct tttcgcgcca gccgttgccc gcgaccgaag gccgaccgag agaacccgag
     4381 cgaaggcgca ggatttgatt cgccgtggcc ggaatttacg ttatggtcca aagggtcggc
     4441 gcggccgtcg ggcggcggtt ccgcaggggc actagacagg gaggagggga cgaaatggtc
     4501 gccaggctgt cgggcgcggt ggtggttgct gtcatcctct ccggcggggc gttggccggc
     4561 ggccaggccg tgctgccgtt cgagcagtcg gcggcgtaca aggtcgacag ccggtccgtg
     4621 ctccatttcg acgattacgc cggcaagatc accgaccagc ggatcaagcg gctgcttcgc
     4681 tcgggcgata tcctgggcga ccgggtgctg ctccagaacg acgtcgcggt ctacttcccg
     4741 gatgggcaca tcgtctcggc gaaggccgag ttccacacct atcgcccggt cgcgggcctg
     4801 ttcccttgga acgtctactc ggccgtccag gtcgagccca atccgaacta ccagtaccgc
     4861 tccgagttcg atgcgctgct cgggccggcc tgcgcggaga ccgtctactc caagggcagc
     4921 aagcggcgcc cgggcgaaag ctacgcgcgc ctgcggatca accgccagaa cgtgctctcg
     4981 ctcgagctcg acagcccggg tctctacgac tatctgaccg gtgagccgtc gcggtcctac
     5041 accgtgacca gcgcgctcga cccgtactat ctgggcgatg cgcaggtggt tcagcagttg
     5101 atgaatacgt gcattgagta ccagcgcacc gcgatcgagc aggcgaaagc gggagacagg
     5161 tgatcggcgg cccgggtggc gggccgcggg gggcgccgtc ggacattcgg cggggcggga
     5221 cgggtgcgga cgatctgccc gtcccttaga tgtgggatag cttgcgtgcc gtccggccgg
     5281 acggccaact cctggcgggg actgtgtttc agtgatgcct ggcgtgcacc ggaccggccg
     5341 ctgtcgatgg tacggctagg ggggcagaca tgtaccggct gatcttggtt gccctgctgg
     5401 cggcgacaac cctgatcgga tgtgtgccgc tcaggcaggg caacactctg ggctacgatt
     5461 tcgcggtcac cgtcgaccgg gccgagctgc ccgaccagtc gtgcaaggtc gacctcacgc
     5521 tgcacaacaa cctcgattcg gatttctcca atttcgagta cgggctcaac ttcttcgaca
     5581 gcctgggccg ctgggccgcc ggccccgtgg tccaaggcga cttcattgcc accggcggcg
     5641 tgtccaagca ggatttcatc ttcccggtcg cctgcgaccg cctggcgagc gccacggtgg
     5701 cgaaacagct gtgcctcgat atcatcggca tggccggccg gcgcctgtgc tacgacatcg
     5761 gccagatcgt gatcaacgac gtggtccgcg ccgaggtgct cggcccgccg gaaggcgtcg
     5821 aggcgccgcc cgccggcaag ccgcgcttct acaccctgtt cttcgattgg gataagtcgg
     5881 acatcaacga cgtcgcccag ctggtgatcg acgcgatcgt cgccgactgg accggcctgc
     5941 ccgactcgct gcagctggtc ggccatgccg accggtcggg tccggtggac tacaaccaga
     6001 agctctcgga gcggcgagtc gcctccgtgg cccaagtgct gatcggccag ggcatcgaag
     6061 ccgagcgcgt ctcgggctac ggcctcggcg agaccgatcc gctggtgccg accccggacg
     6121 gcgtgcgcga gccgcgcaac cgccgcgtgg tggtgacgct cgagacccgc tagcgccggt
     6181 cgtcgacggc gaccaggccc gagggcgtca ccgccctcgg gcctgtcccc tcaccgcccg
     6241 gctgtcagat gtcggcgtag cacttgatct cggcgcgggc gccgggatgg gtgaccgcgc
     6301 cgttcaatgc cgacccgacc agctgggtgt acttccacag ctcgcccgag cggtagtcgt
     6361 gctcgcgtgg cgcccatgac ttgcggcgct cggcgagctc ccgatcggac agctccacgt
     6421 cgagctgccc cgcagccgcg tcgatgacca cgacgtcgcc gtccttgagc aggccgatcg
     6481 gaccgcccac cgccgcctcg gggccgacat ggccgacgca gaagccgcgg gtcgcgcccg
     6541 agaaccggcc gtcggtgatc agcgccacct tgtcgcccat gccctggccg tagatcgccg
     6601 cggtggtcgc cagcatctcg cgcatgcccg ggccgccctt tggcccctcg tagcggatca
     6661 cgagcacctc gccctccttg tagtcgcgcc gctcgaccgc ggcgaaggcg tcctcctcgc
     6721 ggtcgaacac ccgcgcgatc ccgcggaacg tgagggtctt catgcccgcg accttgacga
     6781 tcgcgccctc gggcgcaagg ttaccttgca ggccgaccac gccgccggtc ggcgacagcg
     6841 ggttgtcggc cgggcgcacc acctcctggt cggtgcggaa cctgacctcg gccaggttct
     6901 cggcgacggt ccggccggtc acggtcatgc agccgccgtt gaggaagccg ccgtcgagca
     6961 gcgccttcat cagcatcggc acgccgccgg cctcgtacat gtccttggcg acgaagcggc
     7021 cggcgggctt gaggtcggcg agataggggg tgcgcttgaa gatctcggcg acgtcgagca
     7081 gcgagaactc gatgccggcc tcgttggcga gcgccggcag gtggagcgcg gcattggtcg
     7141 atccacccga ggccgcgacc actgtggccg cgttctcgag cgcttcccgc gtgacgatgt
     7201 cgcgcgggcg gatgccggcc tcgagcaagg ccatgacctg gcgcccggca tcgtcggcgt
     7261 actcgtcgcg gctctcgtag ggtgccggca ccgcggtcga ggccggcagc gcgagaccga
     7321 gagcctcgga tacgcaggcc atggtgttgg cggtgaactg gccgccgcac gagccgcccg
     7381 aggggcaggc gacgtgctcg agctggaaca gctcggcgtc ggtcatcttg ccggccgagt
     7441 gcattcccac cgcctcgtag acgtcctgga cggtgacgtc gcggccctgg aaccggcccg
     7501 gcaggatcga gccgccatag atgaacacgc tcgacacgtt cagccgcagc atcgccatca
     7561 tcatccccgg cagcgacttg tcgcagccgg cgatgccgac cagcgcgtcg taggcgtggc
     7621 cgcgcatggt gagctcgacc gtgtccgcga tcgcctcgcg gctgaccagc gaggccttca
     7681 tgccctggtg gcccatggcg atgccgtcgg tcaccgtgat ggtggtgaac tcgcgcggcg
     7741 tgccgccggc gaacaccacg ccgcgcttgg ccgcctgcgc ctggcgcctg agcgtgatgt
     7801 tgcacggcgc ggtctcgttc caggtggtgg cgacgccgac gaacggctgg tggacctcct
     7861 tgtcgctcag ccccatggcg taatagaacg accggtgcgg cgcgcgctcg ggcccctcgg
     7921 tcgcgtgtcg gctcggcagc ttgctcttgt cgatgcggct catctcgccc cttcccttcg
     7981 ctgttcggcc cggccggagc cggccccgcc tgcggacggg gacgaatcat aagcatggcc
     8041 gcgccgatgc ccagcggaat cgcggccgcg cactggcgtg cgccgtccgg cgacctatct
     8101 cggttgcagg aggaggcccg gtcgatgccc gtgctgcgcg cgttcgtgct cgtcctgctg
     8161 gcgctcggcg ccggcctgcc gcgacctgcc ggcgccgacc tggtcaagcc gttgctgtgc
     8221 ccgcaggccg agatcctgtg cctgcagaag ctcggctaca actggcagaa atgcggcacc
     8281 ctcaccgggc ccggcggcgg cgcccaggag tttccgctgt gcccggccgc ggacggctac
     8341 gcctgcgtgc cgtgctggtt cggctcgctc gccgagaccg cggcccagtg ccgcgcgcgc
     8401 ttcgggcccg actgcggcca cttcgtcgag cacgacgcgc gctggcgcgg cgaatggggc
     8461 gcgttcgcct ggccggagaa ttcgtcgctg ttcctgctgt tcggcgccgg cccgcactga
     8521 ctcctcgagg ggctcagacc gcgctcaggc gtgccaccgc ctgatcgagc ttgcgtcgcg
     8581 acaggtcggc ctcggcgcgg cgctcgcgct gctctgcgat cacgtcctcg ggcgcgcggg
     8641 cgaggaactg ctcgttcgac agcttggtat ccagcttggc gatctccgag atgactttct
     8701 tgag
//
LOCUS       DASWHU010000285        16010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1653408, whole genome shotgun sequence.
ACCESSION   DASWHU010000285 DASWHU010000000
VERSION     DASWHU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16010
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653408"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1820
                     /locus_tag="VGB88_10550"
     CDS             18..1820
                     /locus_tag="VGB88_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9647929.1"
                     /translation="MAAPILTLRSVAVRFGPEPVFADADLVLGARDRLCLVGRNGSGK
                     STLMALIAGRLEPDAGERWARPGLGIGVLDQAVHARPGETVADFVAAGGAREAHAIAA
                     ALAPLAVAPERAMDSLSGGEARRAALARALAGAPELLLLDEPTNHLDLPAIEWLEQWL
                     ASYRGALVVVSHDRALLLGTAGRLGWLDRGRLRLLDGGYDRFDAWREQVYAEDARAQS
                     RIDQRLKAEADWLRHGVTARRRRNQGRLRALEALRERRRALPRIAGAPALALAEAATS
                     GKLAIEAVHVTKRLGGRMVIRDLSLTVMRGERIAITGPNGAGKTTLLGLLSGALAPDE
                     GAVKLGAGVQAVVFDQRREALDGARTVTEILCPAGGDQVTVRGRRRHVSGFLRDFLFD
                     PAQARSPVSSLSGGEKSRLLLAALFARPSNLLVLDEPTNDLDLDTLELLQDVLADYDG
                     TVLMVSHDRTLIDRLATVTLWLAGEGRVEAVVGGFAELARLRQSVPAATRRARARAAA
                     APQRPRSPRRGLGYKDQRELDLLPQRIAELTAEIAALERDLAEPDRYRREPGALAALG
                     ARHRDARQALAAAEDRWLELAARAEALGADRTAP"
     gene            1855..2238
                     /gene="rnpB"
                     /locus_tag="VGB88_10555"
     ncRNA           1855..2238
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGB88_10555"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2647..3132
                     /locus_tag="VGB88_10560"
     CDS             2647..3132
                     /locus_tag="VGB88_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647930.1"
                     /translation="MAFFVGSHEQRIDRKGRVSVPVLFRDVLGKDASFYAFPAFSHHG
                     VLCHTANYMDTLCTDVDDLATFSDEQDHLSWAIFGMSHVLTPDKEGRVSLPGALCQHA
                     EIEDMVVFVGTGRSFQLWAPAHYRVQQSQVRDRVLADRQALRRRPQANPSPVRPVESG
                     T"
     gene            3150..4118
                     /gene="rsmH"
                     /locus_tag="VGB88_10565"
     CDS             3150..4118
                     /gene="rsmH"
                     /locus_tag="VGB88_10565"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9647931.1"
                     /translation="MRSGHVPVLLNEVVAALAPRAGALYVDATFGGGGTSEALLEAAA
                     CTVIAIDRDPDAAARGGALANRYRGRFEFAAGRFGDIERLLGDRAARGVDGGIVFDLG
                     VSSYQLAEPARGFSFRADGPLDMRMDKSGPSAADVVNDTGEAELADILRRFGEERRAR
                     RVAAAIAKARRERPLTRTGELAEVIRGALPPAQGGIDPATRSFQALRIYVNDELGELE
                     RALCAAERLLVAGARLVVVSFHSLEDRIVKRFLARRARPAPLSSRHSPEARAGRDAGA
                     EAPSFRLLTRRAVRPSAAEMAANRRARSARLRAAERTAAPAWREAA"
     gene            4118..4429
                     /locus_tag="VGB88_10570"
     CDS             4118..4429
                     /locus_tag="VGB88_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647932.1"
                     /translation="MRASTVLAALALALAAAGMYQLKYEVAALEAEKQATARALAGER
                     ETIHVLAAEWAYLNRPERLAELAQRHLDLVPLDGARVTSFADLPRRDTELAAAAEEAP
                     R"
     gene            4426..6141
                     /locus_tag="VGB88_10575"
     CDS             4426..6141
                     /locus_tag="VGB88_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9647933.1"
                     /translation="MTHEALPDAVRLEGAGKRALEVGRTRLVIGATLFALGFLGLGLR
                     LVEVSVLGGGDDAGRSGGSAAAGAQRADIVDRNGVVLATNVESHALYADARLVRDAPA
                     LARRLAEILPDLDPEQAARGLASGRSFVWIRRQLSPRQVYAVNRLGEPGLRFLPAQRR
                     VYPLGPLVSHVLGYVDVDNRGLGGIERRFDRELQADPAAPLALSLDIRFQHVLRAELA
                     AALAEFKAIGAAGVILDIASGEVLAMVSLPDFDPNRPATDAGGDDARFNRAALGRYEM
                     GSTFKTFTAAMALDSGTVALTDGFDASQPLHIARFTIRDFHPEGRWLSVPEIFMYSSN
                     IGAARMALAVGAERQRDYLGRFGLLAPSPVELPEVGEPITPDVWREVTTATIGFGHGI
                     AISPLQMATAVASLVNGGTAVTPTVLLRRGAPAAGAQVVSPRTSHQMRQLLRLAVKLG
                     TGKRAEVEGYLVGGKTGTAEKSGKRGYDRRRLISSFVGAFPIDRPRYVVFAVLDEPKG
                     TERTLGYATGGWTAAPLVARVVARIAPLAGIAPRSAPLPGEDGDDGKPLLVTPDGTEI
                     RLASF"
     gene            6128..7621
                     /locus_tag="VGB88_10580"
     CDS             6128..7621
                     /locus_tag="VGB88_10580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9647934.1"
                     /translation="MRLSELARDGPMVDMQGAPDTDIAGLACDSREVRPGFLFAALPG
                     ARTDGARFIDDAIRRGARAVLGPPAEAARARAGRIAFIADANPRRRYALMAARFFPAQ
                     PATLVAVTGTNGKTSVAAFACALWRRLGRQAASLGTLGVDAPGLRRPGALTTPDPVAL
                     HRLLDDLARAGVDHAVIEASSHGLAQHRCDGVRLVAAAFTNLTRDHLDYHGTEDTYFA
                     AKARLFAELLPAGAAAVLNRDSPRYPELAALCARRGHRLVGYGRDAEAGDAPEIALID
                     STAAPDGLALTLRIGRTRHRIDTRLVGGFQAHNLMCALALVIACGEDAARAAAAAEGI
                     AAPRGRLERVARLADGAPIFVDYAHTPDALRAVLEAVRGHVQGRLHLVFGAGGDRDPG
                     KRPQMGAVAAALADRVIVTDDNPRSEQPAAIRAAILAAAPGAREIADRALAIAEAVGA
                     LRPGDALVIAGKGHETGQIIGERTIPFDDAEVARAAVAALAGGGDGA"
     gene            7618..9066
                     /gene="murF"
                     /locus_tag="VGB88_10585"
     CDS             7618..9066
                     /gene="murF"
                     /locus_tag="VGB88_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9647935.1"
                     /translation="MSAPALWTAAEVAAATGGRSTGAWTATGVSIDSRSLAPGDLFFA
                     LAGPTFDGHDFARAALDAGAAAAVVARIPAALEDARALVTVDDTLDALELLGAAGRAR
                     ARARVVGVTGSVGKTGTKEMCAATLAAFGTVHASPGSYNNQVGVPLTLARLAPATGFA
                     VIEMGMNHTGEIAALTRIARPHVAIITGIEAVHLEFFAGLHRIAQAKAEIFDCIPAPA
                     AAVLNRDNACFRQLAQAARRAGVERVIGFGALPDCEVRLVDYAAAPGDNRIEAEVDGR
                     ALAYTLGADGRHWALNSLAALAAVHALGRDVEAAAARLAGVATPPGRGARTTLALAGG
                     RRVILVDDAYNASPASMRAAFEALAAIPVPRGGRRLAVLGDMLELGAESERLHAALAL
                     DIERANIDVVITVGSFMAALNDALPAARRGPHAGSPEAVAELLPEVLHDGDVLLVKGS
                     HASAMYRLVDALRRLDTSGATNPSRCAAAVEG"
     gene            9081..10169
                     /gene="mraY"
                     /locus_tag="VGB88_10590"
     CDS             9081..10169
                     /gene="mraY"
                     /locus_tag="VGB88_10590"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9647936.1"
                     /translation="MLYNLLAPLADQFSLFNLFRYLTFRTGGAIITALIVSFVLGPIV
                     IRWLRTRQGTGQPIRTDGPARHLEAKQGTPTMGGFLILLALTAGTLLWADLANRFVWV
                     VLLVTLGFGAVGFVDDYLKVARRSHRGLPARLKLLIQFAIALLATVWIVRLMPGAVAT
                     ALALPFLKNVLLQLGWFFVPFAMLVIVGASNSVNLTDGLDGLAIVPIMIAAACFGFIS
                     YLAGNTVYANYLQIHGVPGSGELAVLCGALVGASLGFLWFNAPPAMVFMGDTGSLSAG
                     GALGAISVITKHELVLAIIGGLFVLEAVSVIVQVVSFKLTGQRVFRMAPLHHHFEQKG
                     WQEPTIVIRFWIIASILALAGLSTLKLR"
     gene            10174..11598
                     /gene="murD"
                     /locus_tag="VGB88_10595"
     CDS             10174..11598
                     /gene="murD"
                     /locus_tag="VGB88_10595"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9647937.1"
                     /translation="MIPVQGYRDATVAVFGLGRSGLAAVQALAAGGAEVLADDDAADR
                     RRAAVALGARATSLAAADWPGIAALVLSPGVPLSHPAPHPVVDAARAAGVEVIGEVEL
                     FARSLRPGGGVCANGPRLVGITGTNGKSTTTALLGHMLARAGRPVQVGGNLGTAALAL
                     EPLGEDGVYVLEMSSYQLDLISSLVFDVAALVNVSPDHIDRHGDMAGYVAAKRRIFAG
                     QGAACTAVIALDDTVTAAIFDDLDRAGAQRLIPVSTRRALERGVYVADGSLIDALDGA
                     PRRITSLDGIATLPGAHNRQNAAVAYAAARALGVAPEAAADGLRSYPGLAHRLELVAT
                     IAGVRFVNDSKATNVAAASQALACYDTIYWIAGGRAKDEPLAPLEPLLGRVAHAFLIG
                     ESAARFAAALEGRVPVTAAGDLARAVAAAADRAARERRAGAVVLLSPACASFDQFRDF
                     EHRGEMFRAAVRRLEDASPGEAAP"
     gene            11595..12719
                     /gene="ftsW"
                     /locus_tag="VGB88_10600"
     CDS             11595..12719
                     /gene="ftsW"
                     /locus_tag="VGB88_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9647938.1"
                     /translation="MTGFTRTDTSLVARWWWTVDRWMLAALALLIGVGAVMVLAASPA
                     VAERLDLDGFYFVRRHLLLLGPALAVMLAASLLSPTGVRRLAVILLAAGLVLMVATLL
                     VGEDVKGARRWLSIAGLSVQPSEIVRPGFVVVAAWLFATRRQYPRFPGDALATVLLGL
                     VLLLLVLQPDFGMAFTLAGVWLAEFFVAGLPMIWIGGLLALGVGGLVAGYVGLDHVHS
                     RVDRFLDPAAGDTYQVDTSLRAFASGGLLGRGPGEGVVKGQLPDAHADFIFAVIGEEF
                     GLIACLVLVGLFAFVVLRGFARLMREEDLFAVIAGAGLLSLFGLQALLNMAVTLRLVP
                     TKGMTLPFVSYGGSSLIALAAGMGMMLALTRWRPRPEAGR"
     gene            12716..13843
                     /gene="murG"
                     /locus_tag="VGB88_10605"
     CDS             12716..13843
                     /gene="murG"
                     /locus_tag="VGB88_10605"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX9647939.1"
                     /translation="MTAAPTFGPVVIAAGGTGGHVMPALALAHGLQSAGFAPALVTDR
                     RGTAFGDELGGIACHTVRAGGLAGRGVIGALRGLIALVLGGFEARALLRRLAPSLAVG
                     FGGYASVPPLLAAASLGVPTLIHEANAVLGRANRLLAPRARAIATAFPDTRRMRAADG
                     KRATRTGNPVRPEIIAARDVPYPALDPSGPVSLLVVGGSQGARLLGRMVPVALARLDP
                     GLRRRLFVAQQCRPEDLEDARRMFAEASIEAEVAPFFDDVGRRLAASHLVIARAGAST
                     VAELACAGRPAVLVPFARAIDNHQEANARMLEAAGGAWVMLESELSADSLAQRLAELL
                     AGAELPAAAARARAFGVPDASERLVALARGIMHPKPEAEAA"
     gene            13840..15294
                     /gene="murC"
                     /locus_tag="VGB88_10610"
     CDS             13840..15294
                     /gene="murC"
                     /locus_tag="VGB88_10610"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9647940.1"
                     /translation="MSKLPLDIGILHFVGIGGIGMSGIAEVMHNLGYRVQGSDLADNA
                     SVRRLRALAIPITIGHAADNLGEASVVVVSSAVKADNVEVAAARAKLLPVVRRAEMLA
                     ELMRLKWSVAVGGTHGKTTTTSMIAALLDAAGLDPTVINGGIINAYGTNARLGAGDWM
                     VVEADESDGSFVRLPATIAVVTNIDREHLDFYGDFDRLRRAFQSFVENIPFYGFAALC
                     IDHPEVQAMIGRISDRRIVSYGLSPQADVRAAEIRAAGEDTHFDVAIERRRPGEGEVR
                     MIRNLVLHMPGAHNVQNALATVVVGVEMGLSEDTIRKALAGFTGVRRRFTRVGEAGGI
                     AVIDDYGHHPVEIAAVLKAARQVVAARGGGRVIAVVQPHRYTRLRDLFEEFCTCFNDA
                     AAVVVADVYPAGEEPIEGIDRDALVEGLRARGHRAVVALEAPEALPETIHGLAVPGDL
                     VVCLGAGSISQWANDLPAGLRGLGQAPTAAGGGR"
     gene            15291..>16010
                     /gene="murB"
                     /locus_tag="VGB88_10615"
     CDS             15291..>16010
                     /gene="murB"
                     /locus_tag="VGB88_10615"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9647941.1"
                     /translation="MMTAARSRLIDRLPAVRGTVRANAPLARFTWFRVGGPAEVLFTP
                     ADLDDLQLLLGQRPAEVPVTVLGVGSNVLIRDGGIDGVVIRLGRAFAAIEAAGMEVRA
                     GAAALDMRVARAAETAGIAGLEFLAGVPGTIGGAVRMNAGAYGHQIGEVIETIDALDE
                     HGERHRVAAGEIAFHYRHSDLPESWICTAATLAGHADSPARIGARMAEITAAREAAQP
                     IRTRTGGSTFTNPPATAAGGLS"
BASE COUNT         1935 a         5971 c         5833 g         2271 t
ORIGIN      
        1 ggcgctagga tgcggcgatg gcagccccga tcttaacgct caggtcggtt gcggtgcgct
       61 tcgggcctga gcccgtgttc gcggacgcgg acctggtgct cggcgcgcgc gaccggctgt
      121 gcctggtcgg gcgcaacggc agcggcaagt cgaccctgat ggcgctgatc gcaggccggc
      181 tcgagcccga tgcgggcgag cgctgggcgc gcccggggct cggcatcggc gtcctcgacc
      241 aggcggtgca tgcgcgcccg ggcgagaccg tcgccgactt cgtcgccgcc ggcggcgccc
      301 gcgaagcgca cgcgatcgcc gccgcgctcg cgcccctcgc cgtcgcgccg gagcgcgcca
      361 tggactcgct gtcgggcggc gaggcgcgcc gtgccgcgct tgcccgcgcg ctcgccggcg
      421 cgcccgagct gctgctgctc gacgagccga ccaaccatct cgacctgccg gcgatcgagt
      481 ggctcgagca atggttggcg agctatcggg gcgcgctggt ggtggtcagc catgaccgcg
      541 cgctgttgct cggcaccgcc ggccgcctcg gctggctcga ccgtggccga ttgcgcctgc
      601 tcgacggcgg ctacgaccgc ttcgatgcct ggcgcgagca ggtctatgcc gaggacgcgc
      661 gcgcccaaag ccgcatcgac cagcggctca aggccgaggc cgactggctg cgccacggcg
      721 tgaccgcgcg ccggcggcgc aaccaggggc gactgcgcgc gctcgaggcg ctgcgcgaac
      781 gccgccgggc gctgccccgg atcgccgggg cgccggcgct ggcgctggcc gaggccgcga
      841 cctcgggcaa gctcgccatc gaggccgtgc acgtcaccaa gcggctcggc ggacggatgg
      901 tcattcgcga cctctcgctg accgtgatgc gcggcgagcg gatcgccatc accggcccga
      961 acggcgccgg caagaccact ctgctcggcc tgctgtccgg cgcgctggcg cccgatgagg
     1021 gcgcggtcaa gctcggcgcg ggcgtccagg cggtggtgtt cgaccagcgc cgcgaggcgc
     1081 tcgacggcgc ccggaccgtg accgagatcc tgtgcccggc gggcggcgac caggtgacgg
     1141 tgcgcggccg gcgccgccac gtcagcggct tcctgcgcga cttcctgttc gatcccgccc
     1201 aggcccgcag cccggtctca agcctgtcgg gcggggagaa gagccggctg ttgctcgccg
     1261 ccctgttcgc gcggccgagc aacctcttgg tgctcgatga gccgaccaac gatctcgacc
     1321 tcgacaccct cgagctgctg caggacgtgc tcgccgacta cgacggcacg gtgctgatgg
     1381 tcagccacga ccgcaccctg atcgaccgcc tggccacggt caccctgtgg ctcgcgggcg
     1441 agggcagggt cgaggcggtg gtcggcggct tcgccgagct cgcccggctg cggcaaagcg
     1501 tcccggcggc gacgcggcgg gcgcgggcgc gcgccgccgc cgcgccccag cgcccgcgct
     1561 caccgcgccg cgggctcggc tacaaggacc agcgcgagct cgatctgttg ccccagcgca
     1621 tcgccgagct gacggccgag atcgccgcgc tcgagcgcga cctcgccgag ccggaccggt
     1681 accgccgcga gcccggcgcg ctcgccgcgc tcggcgcccg ccaccgcgac gcgcgccagg
     1741 cgctggccgc ggccgaggac cgctggctcg agctcgcggc gcgcgccgag gcgctcggcg
     1801 ccgaccgcac cgccccttga cccgcgcccg ccgaggctct agctgatagg cgcgccagac
     1861 ggccggacgg ccgctccgct caggcggaga ggaaagtccg ggctccacgg gaacgcggtg
     1921 ccgggtaatg cccggcgggg gcgaccccag ggaaagtgcc acagagagca gaccgcccgc
     1981 ccgcgcaagc cggcgggcaa gggtgaaagg gtgcggtaag agcgcaccgc gtcgccggcg
     2041 acggcgacgg cacggcaaac cccaccggga gcaagaccaa atagggacgg cgcgccccgc
     2101 gttcgcgcgg gcaggccggg tttccgcggc cgccgtccgg gtaggtcgcg cgaggcgccc
     2161 ggtaacgggc gtcccagatg aatggccgtc gcccgcgtca tgcgggtaca gaacccggct
     2221 tacaggccgt ctggcgctcg ctctacaact tgtgccgcgg cgcgaaggcg cgcgctcaac
     2281 atgttgtcgt cttttgcgat gtcgtgaccg atactgcgtg ccacaggatc gcgcgcccgg
     2341 gctataatag aacataacaa gaacatattt tagccacaat cgatgcctag agtggccacc
     2401 tggtcgctcc taccagggct agacctcaca tttcgggcgc cggcgacgcg gcagcgactt
     2461 cgctgacgaa gtcttgaaat accccatggg aagactggct ggtcccattg acgcccatat
     2521 attcccatgt tatcccatcg tctcccacac gggcgagccg gtgggcggcc gcgtcgggga
     2581 cggccgagac ggcgggcggc ggccggtgcg gggacttccg gggtgatccc gtgaaggggg
     2641 gaacgagtgg catttttcgt cggcagccac gagcagcgca tcgaccgcaa gggccgcgtg
     2701 tcggtgccgg tgctgtttcg cgacgtgctc ggcaaggacg cctcattcta cgcttttccc
     2761 gcctttagtc atcacggcgt cttgtgccac acggccaatt acatggacac gctctgcacc
     2821 gacgtcgatg accttgccac gttttcggac gaacaggatc acctgtcgtg ggcgatcttc
     2881 ggcatgagcc atgtgctcac acctgacaag gaagggcgcg ttagcctccc tggcgcgctc
     2941 tgtcagcacg ccgagatcga agacatggtg gtgtttgtcg gcaccggccg gtccttccaa
     3001 ttgtgggctc ctgcgcatta cagggtacag cagtcgcagg ttcgggaccg cgtcctcgcc
     3061 gaccgtcagg cgctgcgtcg gcgcccccag gccaacccca gtccagtccg gcctgtggag
     3121 agtggcacgt gaccggaacg tctccctccc tgcgttccgg ccacgtgccc gtcttgttga
     3181 atgaggtcgt ggccgcgctt gcgccgcgcg cgggcgcgct ctacgtcgat gccacctttg
     3241 gcggcggcgg caccagcgag gccttgctcg aggccgcggc gtgcacggtg atcgcgatcg
     3301 accgcgatcc cgacgcggcg gcgcgcggtg gtgcgctggc taatcgttac cgcggccgat
     3361 ttgaattcgc tgcgggcagg ttcggggaca tcgagcgcct gctcggcgac cgcgcggcgc
     3421 gcggcgtcga cggcggcatc gtgttcgacc tcggcgtctc gtcctaccag cttgccgagc
     3481 ccgcgcgcgg cttcagcttc cgcgccgacg gcccgctcga catgcgcatg gacaagtccg
     3541 gcccgagcgc ggcggacgtg gtcaacgaca ccggcgaggc cgagctcgcc gatatcctga
     3601 ggcgcttcgg cgaggagagg cgggcgcgcc gcgtcgccgc ggcgatcgcc aaggcgcgcc
     3661 gcgagcggcc gctcacgcgc accggcgagc ttgccgaggt gatccgcggc gcgctgccgc
     3721 cggcgcaggg cggcatcgat ccggcgacgc gcagctttca ggcgctgcgc atctacgtca
     3781 acgacgagct cggcgagctc gagcgagcgc tctgcgccgc cgagcggctg ctggtcgcgg
     3841 gcgcgcgcct ggtggtggtc tcgttccatt cgctcgagga ccgcatcgtc aagcgcttcc
     3901 tcgcccgtcg cgcgcggccg gcgccgctga gctcgcgcca cagccccgag gcgcgcgcgg
     3961 gccgcgacgc cggtgccgag gcgccgtcgt tccggctgct gacccggcgc gcggtcaggc
     4021 cgtcggcggc ggagatggcg gccaaccggc gcgcgcgctc ggcgcgcttg cgggccgccg
     4081 agcgcaccgc ggcgccggcc tggcgggagg cggcgtgatg cgcgcctcga ccgtgctcgc
     4141 cgcgctcgcc ctcgccctcg ccgccgccgg catgtaccag ctcaaatacg aggtcgccgc
     4201 gctcgaggcc gagaaacagg cgacggcgcg cgcgctcgcc ggcgagcgcg agaccatcca
     4261 cgtgctcgcc gccgagtggg cctatctcaa ccgtccggaa cggctggcgg agctcgccca
     4321 gcgccatctc gacctggtgc cgctcgacgg tgcgcgcgtc accagctttg ccgacctgcc
     4381 gcgccgcgac accgagctcg ccgccgccgc cgaagaggcg ccgcgatgac ccacgaggcc
     4441 ttacccgacg cggtccggct cgagggcgcc ggcaagcgcg cgctcgaggt cggccggacc
     4501 cggctggtga tcggcgcgac cctgttcgcg ctcggcttcc ttggcctcgg cctgcgcctg
     4561 gttgaagtgt cggtgctcgg cggcggcgac gacgccgggc gcagcggcgg cagcgcagcc
     4621 gcgggcgcgc agcgcgccga catcgtcgac cgcaacggcg tggtgctggc aaccaacgtc
     4681 gagtcgcacg cgctctatgc cgatgcccgg ctggtgcgcg acgcgccggc gctggccagg
     4741 cgcctggccg agatcctccc cgacctcgac ccggagcagg ccgcgcgcgg ccttgccagc
     4801 gggcgcagct tcgtctggat ccgccgccag ctgagcccgc gccaggtcta cgcggtcaac
     4861 cggctcggcg agccggggct gcgcttcctg cccgcccagc gccgggtcta tccgctcggg
     4921 ccgctggtct cgcacgtcct cggctatgtc gacgtcgaca accgcgggct cggcggcatc
     4981 gagcgccgct tcgaccgcga actgcaggcc gacccggcgg caccgcttgc gctgtcgctc
     5041 gacatccgct tccagcacgt gctgcgcgcc gagctcgccg cggcgctcgc ggagttcaag
     5101 gcgatcggcg ccgcaggcgt gatcctcgac atcgccagcg gcgaggtgct ggcgatggtc
     5161 agcctgcccg acttcgaccc caatcgcccg gcgaccgatg cgggcggcga cgatgcgcgc
     5221 ttcaaccgcg ccgcgctcgg ccgctacgag atgggctcga cgttcaagac cttcaccgcc
     5281 gcgatggcgc tcgactcggg cacggtcgcg ctcaccgacg gcttcgatgc ctcccagccg
     5341 ctgcacatcg cgcgcttcac catccgcgac tttcatcccg aaggccgttg gctgtcggtg
     5401 cctgagatct tcatgtactc atcgaacatc ggcgccgcca ggatggcgct cgcggtcggg
     5461 gccgagcgcc agcgcgacta tctggggcgg ttcggcctgc tggcgccgtc gccggtcgag
     5521 ctgcccgagg tcggcgagcc gatcacgccc gacgtctggc gcgaggtgac gacggcgacc
     5581 atcggcttcg gccacggcat cgccatcagc ccgctgcaga tggccaccgc ggtcgcctcg
     5641 ctggtcaacg gcggcaccgc ggtgacgccg accgtgctgc tcaggcgcgg cgcgccggcc
     5701 gcgggcgcgc aggtggtctc gccccgcacc tcgcaccaga tgcgccagct actgcgcctg
     5761 gcggtcaagc tcggcaccgg caagcgggcc gaggtcgagg gctatctggt cggcggcaag
     5821 accggcaccg cggaaaagtc gggcaagcgc ggctacgacc gccggcggct gatctcgtcg
     5881 ttcgtcggcg ccttcccgat cgaccggccg cgctacgtcg tgttcgcggt gctcgacgag
     5941 cccaagggca ccgagcgcac cctcggctac gctaccggcg gctggacggc ggcgccgctg
     6001 gtcgcgcgcg tggtcgcgcg catcgcgccg ctggccggga tcgcgccgcg cagcgcgccg
     6061 ttgccgggcg aggacggaga cgacggcaag ccgctcctgg ttacgcccga cggcaccgag
     6121 atccgccttg cgtctttctg agctcgcgcg cgacgggccg atggtcgaca tgcagggcgc
     6181 gcccgacacc gacatcgcgg ggcttgcctg cgacagccgc gaggttcgcc ccggcttcct
     6241 gttcgccgcc ttgccgggcg cgcgcaccga cggcgcgcgc ttcatcgacg acgccatccg
     6301 ccgcggcgcg cgcgcggtgc tcggcccgcc cgccgaggcg gcgcgggcgc gcgccgggcg
     6361 catcgccttc atcgccgatg ccaacccgcg ccggcgctac gcgctgatgg cggcgcgctt
     6421 cttccccgcc cagcccgcca ccctggtcgc ggtgaccggc accaacggca agacctcggt
     6481 tgcggcgttc gcgtgtgcgc tgtggcgccg gctcggccgg caggcggcga gcctgggcac
     6541 gctcggcgtc gacgcccccg ggctccgccg gccgggcgcg ctgaccacgc ccgatccggt
     6601 cgcgctccat cgcctgctcg acgacctcgc gcgcgccggc gtcgaccacg cggtgatcga
     6661 ggcgtcgagc cacgggctcg ctcagcaccg ctgcgacggc gtgcgcctgg tcgccgccgc
     6721 gttcaccaac ctgacccgcg accatctcga ctatcacggc accgaggaca cctacttcgc
     6781 cgccaaggcg cggctgttcg ccgagctctt gccggccggg gccgccgcgg tgctcaatcg
     6841 cgactcgccc cgctacccgg agctcgcggc attgtgcgcg cgccgcggcc accgactggt
     6901 cggctacggc cgcgacgccg aggcgggcga tgcgcccgag atcgcgctca tcgactcgac
     6961 cgccgcgccc gacggcctgg cgctgacgtt gcgtatcggc aggacgcgcc accgcatcga
     7021 cacccgcctg gtcggcggct tccaggcgca caacctgatg tgcgcgctgg cgctggtcat
     7081 cgcctgcggc gaggacgcgg cgcgcgcggc cgccgccgcc gaagggatcg cagcgccgcg
     7141 cggccggctc gagcgcgtcg cgcgccttgc cgacggcgcg ccgatcttcg tcgactacgc
     7201 gcacacgccg gatgcgctgc gcgcggtgct cgaggcggtg cgcggccacg tccagggccg
     7261 cctgcatctg gtgttcggcg ccggcggcga ccgcgacccc ggcaagcggc cgcagatggg
     7321 cgcggtcgcg gcagcgctcg ccgaccgggt catcgtcacc gacgacaacc cgcgcagcga
     7381 gcagccggcg gcgatccgcg ccgcgatcct ggccgcggcg ccgggcgcgc gcgagatcgc
     7441 cgatcgcgcc ctggcgatcg ccgaggcggt cggcgccctg cgccccggag acgccctggt
     7501 gatcgcgggc aagggacacg agaccgggca gatcattggc gagcgaacga ttcccttcga
     7561 cgatgccgag gtggcgcggg cggcggtggc ggcgctcgcc ggcggcggag acggggcatg
     7621 agcgcgccgg cgctgtggac cgcggccgag gtcgccgccg ccaccggcgg gcggagcacc
     7681 ggcgcctgga ctgcgaccgg ggtctcgatc gacagccgct cgctcgcgcc gggcgacctg
     7741 ttcttcgcgc tcgcgggacc gaccttcgac ggccacgact tcgcccgcgc ggcgctcgac
     7801 gccggcgccg cggcggcggt ggtcgcgcgt attccggcgg cgctcgaaga tgcgcgcgca
     7861 ctggtgacgg tcgacgacac gctcgatgcg ctcgaattgc tcggcgccgc cgggcgggcg
     7921 cgcgcgcgcg cgcgcgtggt cggcgtcacc ggcagcgttg gcaagactgg caccaaggag
     7981 atgtgcgccg cgacgctcgc cgcgttcggc accgtccacg ccagccccgg cagctacaac
     8041 aaccaggtcg gggtgccgct gacgcttgcc aggctcgcgc cggcgaccgg cttcgcggtc
     8101 atcgagatgg gcatgaacca taccggcgag atcgccgcgc tgacccgcat cgcccggccc
     8161 catgtcgcaa tcattaccgg catcgaggcg gttcatctcg agttcttcgc cggcctgcac
     8221 cgcatcgccc aggccaaggc ggagatcttc gactgcatcc ctgcgcccgc cgcggcggtg
     8281 ctcaaccgcg acaatgcctg cttccgccag cttgcccagg cggcccggcg cgccggcgtc
     8341 gagcgcgtga tcggcttcgg cgcgctgccc gactgcgagg tgcggctggt cgattacgcc
     8401 gcggcgccgg gcgacaaccg catcgaggcc gaggtcgacg ggcgcgcgct cgcctacacc
     8461 cttggcgccg acggccgcca ttgggcgctc aacagcctgg ccgcgctcgc cgcggtgcac
     8521 gcgctcgggc gcgacgtcga ggccgcggcc gcgcgcctgg ccggcgtcgc cacgccgccc
     8581 ggacgcggtg cgcgcacgac cctggcgctc gccggcgggc gccgcgtcat cctggtcgac
     8641 gatgcctaca acgcgagccc ggcctcgatg cgcgccgcgt tcgaggcgct ggccgcgatc
     8701 ccggtgccgc gcggcgggcg caggctcgcc gtgctcggcg acatgctcga gctgggcgct
     8761 gagtccgaac gcctgcacgc cgcgctcgcg ctcgacatag agcgcgccaa tatcgacgtc
     8821 gtgatcacgg tcggctcgtt catggcggcg ctcaatgacg cgctgccggc ggcacgccgc
     8881 ggcccccatg ccggctcgcc cgaggcggtg gcggagctcc tgcctgaggt cttgcacgac
     8941 ggcgacgtgc tgctggtcaa gggctcgcac gccagcgcca tgtaccggct ggtggacgcg
     9001 ctccgccgcc tcgacacctc cggcgccacc aatccgtcgc gctgcgccgc ggcggtcgag
     9061 ggctgaggcg ggaggcggca atgctgtaca acctgctggc gccgctcgcc gaccagttca
     9121 gcctgttcaa cctgttccgc tacctgacct ttcgtaccgg cggggcaatc attaccgcgc
     9181 tcatcgtcag cttcgtgctc gggcctatcg tgatccgctg gctgcgcacg cgccagggca
     9241 ctggccagcc gatccggacc gacggcccgg cgcgccatct cgaggccaag cagggcacgc
     9301 cgaccatggg cggcttcctg attttgctgg cgctgaccgc cggcaccctg ttgtgggccg
     9361 acctcgccaa ccggttcgtc tgggtggtgc tgctggtgac cctcgggttc ggcgccgtcg
     9421 gcttcgtcga cgactatctc aaggtagcgc ggcgcagcca tcgcggcctg cccgcgcggc
     9481 tcaagctgct gatccagttc gcgatcgcgc tcttggccac ggtgtggatc gtgcgcctga
     9541 tgccgggggc cgtggccacg gcgctggcgc tgccgttcct caagaacgtg ctgctccagc
     9601 tcggctggtt cttcgtgccg ttcgcgatgc tggtgatcgt cggcgcgtcg aactcggtca
     9661 acctgaccga cggcctcgac gggctcgcca tcgtgccgat catgatcgcc gccgcctgct
     9721 tcggcttcat ctcgtacctg gccggcaaca cggtctacgc caactacctg cagatccacg
     9781 gcgtgcccgg gtcgggcgag cttgccgtgc tgtgcggcgc tctggtcggc gccagcctcg
     9841 gcttcctgtg gttcaacgcg ccacccgcga tggtgttcat gggcgacacc ggctcgctgt
     9901 cggccggcgg cgcgctcggc gcaatcagcg tgatcaccaa gcacgagctg gtgctggcga
     9961 tcatcggcgg cctgttcgtg ctcgaggccg tgtcggtgat cgtccaggtg gtgtcgttca
    10021 agctcaccgg ccagcgcgtg ttccgcatgg cgccccttca ccaccacttc gagcagaagg
    10081 ggtggcagga gccgaccatc gtcatccggt tctggatcat cgcctcgatc ctggcgctcg
    10141 ccgggctgtc gacgctcaag ctgaggtgag gcgatgatcc cggtgcaggg ctatcgcgat
    10201 gccacggttg cggtgttcgg cctcggccgc agcggccttg ccgcggtgca ggcgctcgcc
    10261 gccggcggcg ccgaggtgct cgccgacgac gacgccgccg accgccgccg ggcggcggtc
    10321 gcgctcggcg cgcgcgcgac ctcgctcgcc gccgccgact ggccgggcat cgccgcgctg
    10381 gtgctgagcc ccggcgtgcc gctcagccac ccggcgccgc acccggtggt tgacgccgcg
    10441 cgcgcggccg gcgtcgaggt gatcggcgag gtcgagctgt tcgcccgcag cctgcgcccg
    10501 gggggcggcg tctgcgccaa cgggccgcgc ctggtcggca tcaccggcac caacggcaag
    10561 tcgaccacca ccgcgctgct cggccacatg ctcgcgcgcg ccgggcggcc ggtccaggtc
    10621 ggcggcaatc tcggcaccgc cgcgctcgcg ctcgagccgc tcggcgagga cggcgtctac
    10681 gtgctcgaga tgtcatccta ccagctcgac ctgatcagct cgctggtgtt cgacgtcgcg
    10741 gcgctggtca acgtctcgcc cgaccacatc gaccgccacg gcgacatggc cggctacgtg
    10801 gcggcgaagc ggcgcatctt cgccggccag ggcgcggcgt gcacggcggt gatcgcgctc
    10861 gacgacacgg tcacggcggc gatcttcgac gacctcgatc gcgccggcgc gcagcgcctg
    10921 atcccggtgt cgacgcggcg ggcgttggag cgcggcgtct atgtcgcgga cgggtcgctg
    10981 atcgacgccc tcgacggggc cccgcgccgg atcacctcgc tcgacggcat cgccaccctg
    11041 cccggcgcgc acaaccgcca gaacgcggcg gtcgcctacg ccgcggcgcg cgcgctcggc
    11101 gttgcgcccg aggccgcggc cgacggcctg cgcagctacc ccggcctcgc ccaccgactc
    11161 gagctggtcg cgaccatcgc cggcgtgcgc ttcgtcaacg acagcaaggc gaccaacgtc
    11221 gccgccgcgt cacaggcgct ggcctgctac gacacgatct actggattgc cggcgggcgg
    11281 gcgaaggacg agccgctggc gccgctcgag cccctgctcg ggcgcgtcgc ccacgccttc
    11341 ctgatcggcg agtcggcagc gcgcttcgcc gcggcgctcg agggtcgcgt cccggttacc
    11401 gccgccggcg acctcgcccg cgcggtcgcc gccgccgccg atcgcgccgc ccgcgagcgg
    11461 cgcgcgggcg cggtggtgct gctgtcgccg gcctgcgcct cgttcgacca gttccgcgac
    11521 ttcgagcacc gcggcgagat gttccgcgcc gcggtccggc gcctcgagga cgcctcgccc
    11581 ggggaggccg cgccgtgacc ggcttcacgc gcaccgacac cagcctggtc gcccgctggt
    11641 ggtggaccgt cgaccgctgg atgctggcgg cgctggcgct gctgatcggc gtcggcgcgg
    11701 tgatggtgct ggcggcgagc ccggcggtgg ccgagcggct cgacctcgac ggcttctact
    11761 tcgtgcgccg gcacctgctg ctgctcgggc cggcattggc ggtgatgctt gccgcctcgc
    11821 tgctgtcgcc gaccggggtg cgccgcctcg ccgtgatcct gctcgcggcc ggcctggtgc
    11881 tgatggttgc cacgctgttg gttggcgagg acgtgaaggg cgcgcgccgc tggctgagca
    11941 tcgccgggct gtcggtgcag ccgtccgaga tcgtcaggcc ggggttcgtg gtggtggcgg
    12001 catggctgtt cgccacccgc cgccagtacc cgcgcttccc gggcgatgcg ctggcgaccg
    12061 tcctcttggg gctggtcttg ctgctgctgg tcctgcagcc cgacttcggc atggcgttca
    12121 ccctggccgg ggtgtggctc gccgagttct tcgtcgccgg cctgccgatg atctggatcg
    12181 gcggcctgct cgcgctcggg gtcggcggcc tggtggcggg ctatgtcggc ctcgaccacg
    12241 tccacagccg cgtcgaccgc tttctcgacc ccgcggccgg cgacacctat caggtcgata
    12301 cctcgctgcg cgcgttcgcg agcggcgggc tgctcgggcg cggcccgggc gagggggtgg
    12361 tcaaggggca gctgcccgat gcccatgccg acttcatttt cgcggtcatc ggcgaggagt
    12421 tcgggctgat cgcctgcctg gtgctggtcg gcctgttcgc gttcgtggtg ctgcgcggct
    12481 ttgcccgcct gatgcgcgag gaggacctgt tcgcggtgat tgccggggcg ggcctgttgt
    12541 cgctgttcgg cctgcaggcg ctgctcaaca tggcggtcac cctccgcctg gtgccgacca
    12601 agggcatgac cctgccgttc gtctcctatg gcggctcgtc gctgatcgcg cttgccgcgg
    12661 gcatgggcat gatgctggcg ctgacccgct ggcggccgcg gccggaggcc gggcgatgac
    12721 cgccgcgccc accttcggtc cggtggtgat cgccgccggc ggcaccggcg gccacgtgat
    12781 gccggcgctc gcgctcgccc acggcctgca gagcgcgggc ttcgcgccgg cactggtcac
    12841 cgaccggcgc ggcaccgcct tcggcgacga gctcggcggc atcgcctgtc acacggtgcg
    12901 cgccggcggc ctcgccgggc gcggcgtgat cggcgcgctg cgcggcctga tcgcgctggt
    12961 cctcggcggc ttcgaggcgc gcgcgctgct caggcggctg gcaccctcgc tcgcggtcgg
    13021 cttcggcggc tatgcctcgg tgccgccgct gcttgccgcc gcgagcctcg gcgtgccgac
    13081 cctgatccac gaggctaacg cggtgctcgg ccgcgccaac cggctgctcg cgccgcgcgc
    13141 gcgcgcgatc gcgaccgcgt tcccggacac ccgccggatg cgcgccgccg acggcaagcg
    13201 cgcgacgcgc accggcaacc cggtgcggcc cgagatcatc gccgcccgcg acgtgcccta
    13261 tccggcgctc gacccgtcgg ggccggtgag cctgctggtg gtcggcggca gccagggcgc
    13321 gcggctgctg gggcgcatgg tgccggtggc gctcgcgcgg ctcgatccgg gcctgcggcg
    13381 ccggctcttc gtcgcccagc agtgccgtcc cgaggacctg gaagatgccc ggcgcatgtt
    13441 tgccgaggca agcatcgagg ccgaggtggc gccgttcttc gatgacgtcg gcagacgcct
    13501 cgccgcgagc catctggtga tcgcgcgcgc cggtgcctcc accgtcgccg agctcgcctg
    13561 cgccggccgc cccgccgtgc tggtgccgtt cgcgcgcgcc atcgacaacc accaggaggc
    13621 gaacgcgcgc atgctcgagg ccgcgggcgg ggcctgggtg atgctcgaat ccgagctctc
    13681 cgccgactcg ctcgcccagc gccttgccga gctgctcgca ggcgccgagc tgcccgcggc
    13741 ggcggcgcgg gcgcgcgcgt tcggcgtgcc cgacgcatcc gagaggctgg tcgcgctggc
    13801 gcgcgggatc atgcacccga agccggaggc agaggcggca tgagcaagct tcccctcgac
    13861 atcggcatcc tgcacttcgt cggcatcggc ggcatcggca tgagcggcat cgccgaggtg
    13921 atgcacaacc tgggctatcg cgtgcaaggc agcgatctcg ccgacaacgc ctcggtgcgc
    13981 cggctgcgcg cgctcgccat accgatcacc atcggccatg cggccgacaa tctgggcgag
    14041 gcctcggtgg tggtggtctc gagcgcggtc aaggccgaca acgtcgaggt ggcggcggcg
    14101 cgcgccaagc tgttgccggt agtccgccgg gcggagatgc tggccgagct gatgcggctc
    14161 aaatggtcgg tcgcggtcgg cggcacccac ggcaagacca ccaccacctc gatgatcgcg
    14221 gcgctgctgg atgcggccgg gctcgacccg accgtgatta acggcggcat catcaacgcc
    14281 tacggcacca acgcccgcct cggcgccggc gactggatgg tggtcgaggc cgacgagtcc
    14341 gacggcagct tcgtccggct gccggcaacc attgccgtgg tcaccaacat cgaccgcgag
    14401 catctcgact tctacggcga cttcgaccgg ttgcggcgcg cgttccagag cttcgtcgag
    14461 aacatcccgt tctacggctt cgcggcgctg tgcatcgacc atcccgaggt gcaggcgatg
    14521 atcgggcgca tctctgaccg gcgcatcgtc agctacgggc tgagcccaca ggccgacgtg
    14581 cgcgcggccg agatccgcgc cgccggcgag gacacccact tcgacgtcgc catcgagcgc
    14641 cgcaggccgg gcgagggcga ggtgcgcatg atccgcaacc tggtcctgca catgccgggc
    14701 gcgcacaacg tccagaacgc gctggcgacg gtggtggtcg gtgtcgagat gggcctctcc
    14761 gaggatacca tccgcaaggc gctggccggg ttcaccggcg tgcgccggcg cttcacccgg
    14821 gtcggcgagg ccggcggcat cgccgtgatc gacgactacg gccaccaccc ggtcgagatc
    14881 gccgcggtgc tcaaggcggc gcgccaggtg gtcgccgccc gcggcggcgg ccgggtgatc
    14941 gcggtggtcc agccgcatcg ctatacccgg ctccgcgacc tgttcgagga gttctgcacc
    15001 tgcttcaacg atgccgcggc ggtggtcgtc gccgacgtct acccggccgg cgaggagccg
    15061 atcgagggca tcgatcgcga cgccctggtc gagggcctgc gcgcgcgcgg ccaccgcgcc
    15121 gtggtcgcgc tcgaggcgcc cgaggcgctg cctgagacga ttcacggcct ggcggtgccc
    15181 ggcgacttgg tcgtgtgtct cggtgccggc tcgatcagcc agtgggcgaa cgacctgccg
    15241 gccggcttgc gcggcctcgg ccaggcgccg accgcggcgg ggggcgggcg atgatgacgg
    15301 ctgcgcgctc gcgcctgatc gaccggctgc cggcggtgcg cggcaccgtg cgcgcgaatg
    15361 cgccgctcgc gcgcttcacc tggttccgcg tcggcggccc ggccgaggtg ctgttcaccc
    15421 ccgccgatct cgacgacctg caactgttgc tcggccaacg gccggccgag gtgccggtga
    15481 ccgtgctcgg cgtcggctcg aacgtactga tccgcgacgg cggcatcgac ggcgtggtga
    15541 tccggctggg gcgcgccttc gccgcgatcg aggcggcggg catggaggtg cgcgcgggcg
    15601 cggccgcact cgacatgcgg gtggcgcgcg cggccgagac ggcgggcatc gccgggctcg
    15661 agttcctggc cggggtgccg ggcacgatcg gcggcgcggt gcgcatgaac gccggcgcct
    15721 acgggcacca gatcggcgag gtcatcgaga ccatcgacgc gctcgacgag cacggcgaac
    15781 gccaccgcgt ggctgccggc gagatcgcct tccactaccg ccacagcgac ctgcccgaga
    15841 gctggatctg caccgcggcg acgctcgcag gccacgcgga ttcgccggcg cggatcgggg
    15901 cgcgcatggc cgagatcacc gccgcgcgcg aggccgccca gccgatccgc acccgcaccg
    15961 gcggcagcac cttcaccaac ccgcccgcca cggccgcggg cggcctcagc
//
LOCUS       DASWHU010000286         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_898299, whole genome shotgun sequence.
ACCESSION   DASWHU010000286 DASWHU010000000
VERSION     DASWHU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898299"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VGB88_10620"
     CDS             complement(<1..571)
                     /locus_tag="VGB88_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX9647942.1"
                     /translation="MAARGAPRLGLAGMLAGLMIAGAVAAGPAAAIGEAQAPAAQAWS
                     FEGIFGRFRHPDVQRGLQVYLQVCANCHGLDLVAYRDLAGIGYSEDQIAAIAAEYEVE
                     DGPDEDGDMFQRPARASDRFVSPYANDKAAAAANGGKAPPDLSLMYKARAGGADYIRA
                     LLTGYEDAPAGIEVPAGAAYNLYFAGHFIV"
     gene            complement(571..1860)
                     /locus_tag="VGB88_10625"
     CDS             complement(571..1860)
                     /locus_tag="VGB88_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9647943.1"
                     /translation="MSELSTGSRLVRWFDYRLPIFTLLNEAVVVYPTPRNLNYWWSFG
                     SLAGLVLVIQILTGIVLSMHYVGHVDFAFDSVELIMRDVNYGWLLRYVHANGGSAFFA
                     VVYIHVFRGLYYGSYKTPREVLWWMGVLILIAMMASAFMGYVLPWGQMSFWAATVITN
                     LFSAIPIWGEDIVTWLWGGFAVGNPTLKRFYSLHYLLPFVIVGLVILHLWALHRFGSN
                     NPVGIDTKGGKDEIPFHPYYTIKDLFGIGVFFIVFFAFVFYAPNYLSHPDNYVRANPL
                     VTPPHIVPEWYFLPYYAILRSITFNVWFIPAKLIGVVLMFGSILLLFFLPWLDTSPVR
                     SARFRPIYKQVFWLLVADTVVLGIVGANAPNAPVFKGNESFQYVNLGQIATAYYFLHF
                     LVIMPLLGRFETPRPVPVSISEPVLAEGGGAAAREGS"
     gene            complement(1873..2409)
                     /gene="petA"
                     /locus_tag="VGB88_10630"
     CDS             complement(1873..2409)
                     /gene="petA"
                     /locus_tag="VGB88_10630"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX9647944.1"
                     /translation="MAETAGAGVHEAGGETRRDFLYIATGMVAGVGTLAALWPLIDQM
                     NPSRDVLALASADVDLSPVEVGQSITVVWRGRPVFVRHRTTAEIEAARDVDESSLPDP
                     QTDAARAEKPEWLVVVGVCTHLGCIPLGNAGDYGGWYCPCHGSHYDTSGRIRKGPAPA
                     NLEVPPYAFLDDHTIRIG"
     gene            2574..>2947
                     /locus_tag="VGB88_10635"
     CDS             2574..>2947
                     /locus_tag="VGB88_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX9647945.1"
                     /translation="MSQAVEIESRKERASAWFAELRDRLCAAFEAVEDALAQGPHVAL
                     APGRFERTPWTRPGGGGGEMTVMRGRVFEKVGVNVSTVHGEFSPEFRAQVPGAADDAR
                     FWASGISLVAHPRSPLVPAAHM"
BASE COUNT          505 a          988 c         1042 g          412 t
ORIGIN      
        1 tgacgatgaa atggccggca aagtacaggt tgtaggcggc cccggccggc acctcgatgc
       61 cggcgggcgc atcctcgtag ccggtgagca gcgcccggat gtagtcggcg ccgccggcgc
      121 gcgccttgta catcagcgac agatccggcg gcgccttgcc gccgttggcc gccgccgccg
      181 ccttgtcgtt ggcgtaaggc gagacgaagc ggtccgatgc ccgcgccggg cgctggaaca
      241 tgtcgccgtc ctcgtccggg ccgtcctcga cctcgtactc ggccgcgatc gcggcgatct
      301 ggtcctcgct gtagccgatc ccggcgaggt cgcgataggc gaccagatcg agcccgtggc
      361 agttcgcgca cacctgcagg tagacctgca ggccgcgctg cacgtcgggg tgccggaagc
      421 gcccgaagat gccctcgaac gaccacgcct gcgcggcggg cgcctgtgcc tcgccgattg
      481 cggccgcggg cccggcggcg acggcgccgg caatcatcag cccggcaagc atccccgcga
      541 gtcccaggcg cggggcgccg cgcgccgcca tcacgatccc tcgcgggccg ccgcgccgcc
      601 gccctcggcg agcaccggct cggagatgct gaccggcacc ggccgcggcg tctcgaagcg
      661 cccgagcagc ggcatgatca ccaggaaatg caggaagtag tacgcggtcg cgatctgccc
      721 caggttgacg tactggaagg actcgttgcc cttgaacacc ggcgcgttcg gggcattcgc
      781 gccgacgatg ccgaggacca cggtgtcggc gaccagcagc cagaacacct gcttgtagat
      841 cggccggaaa cgggcgctgc gcaccggcga ggtgtcgagc cagggcagga agaacagcag
      901 caggatcgag ccgaacatca gcaccacgcc gatcagcttg gcggggatga accagacgtt
      961 gaacgtgatc gagcgcagga tcgcgtagta gggcaggaaa taccactcgg gtacgatgtg
     1021 cggcggagtg accagcgggt tcgcgcgcac atagttgtcg ggatggctca gatagttggg
     1081 cgcatagaac acgaacgcga agaacacgat gaagaacacg ccaatgccga acaggtcctt
     1141 gatcgtgtag tagggatgga acgggatctc gtccttgccg cccttggtgt cgatgccgac
     1201 cgggttgttg gagccgaacc ggtgcagcgc ccacagatgc aggatgacca ggccgacgat
     1261 cacgaacggc agcaggtaat gcaggctgta gaagcgcttg agcgtggggt tgccgaccgc
     1321 gaagccgccc cacagccaag tcacgatgtc ctcgccccag atcgggatcg ccgagaacag
     1381 gttggtgatc acggtcgccg cccagaagct catctggccc cagggcagca catagcccat
     1441 gaacgcgctg gccatcatcg cgatcaggat cagcaccccc atccaccaca gcacctcgcg
     1501 cggcgtcttg tacgagccgt aatagaggcc gcgaaagacg tggatgtaga ccacggcgaa
     1561 gaacgccgag ccgccgttgg cgtgcacgta gcgcagcagc cagccgtagt tgacgtcgcg
     1621 catgatcagc tcgacgctgt cgaaggcaaa gtcgacatgt cccacatagt gcatcgacag
     1681 cacgatgccg gtgaggatct ggatcaccag caccaacccg gcgagcgagc cgaagctcca
     1741 ccagtaattg aggttgcgcg gcgtcggata gacgaccacc gcctcgttca gcagggtgaa
     1801 gatcggcagc cggtagtcga accacctgac caagcggctg cccgtcgaga gctcgctcat
     1861 gtcgcggcgc cgctagccga ttcggatcgt atggtcgtcg aggaaggcgt agggcggcac
     1921 ctccaggttg gccggcgccg gccccttgcg gatcctgccc gaggtatcgt agtgcgagcc
     1981 gtggcacggg cagtaccagc cgccgtaatc gccggcattg ccgagcggta tgcagccgag
     2041 atgggtgcac acgccgacca ccaccagcca ttccggcttt tcggcgcgcg cggcgtcggt
     2101 ctgcgggtcg ggcagcgagc tttcgtcgac gtcgcgcgcg gcctcgatct cggcggtggt
     2161 gcggtggcga acgaacaccg gccggccgcg ccagaccacg gtgatcgact ggccgacctc
     2221 gaccggcgac aggtcgacgt cggcgctggc cagcgccagc acgtcgcgcg acgggttcat
     2281 ctggtcgatc agcggccaca gcgccgcgag cgtgccgacg ccggcgacca tgccggtggc
     2341 gatgtagagg aagtcgcggc gcgtctcgcc gcccgcttcg tgcacccccg ctccagcggt
     2401 ctccgccatg cgcgccccgc atcgtggccg aaaaaagcgc ggcctttgtg ccccaatcca
     2461 cccgcgatgt ccaggcccct gcccggccgc atcgggccgc gatcgggcac cgttgcgcgg
     2521 ggcgcgcgcg cgatgctagg ggtggcggcg gcaggggcgc gcggagcgcg gtgatgagcc
     2581 aggcagtcga gatcgagtcg cgaaaggagc gggcgagcgc gtggttcgcc gagctgcgcg
     2641 atcgcctctg cgccgcgttc gaggcggtcg aggacgcgct cgcccaaggc ccgcacgtcg
     2701 ccctcgcgcc gggccgattc gagcgcacgc cctggacacg cccgggcggc ggcggcggcg
     2761 agatgacggt gatgcgcggg cgcgtgttcg agaaggtggg ggtcaacgtc tcgaccgtcc
     2821 acggcgagtt ctcgcccgag ttccgcgccc aggtgccggg tgccgccgac gatgcgcggt
     2881 tctgggcgag cggcatctcg ctggtcgcgc atccgcgctc gccgctggtg ccggcggcgc
     2941 acatgaa
//
LOCUS       DASWHU010000287         8023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_154922, whole genome shotgun sequence.
ACCESSION   DASWHU010000287 DASWHU010000000
VERSION     DASWHU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154922"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="amrB"
                     /locus_tag="VGB88_10640"
     CDS             complement(<1..599)
                     /gene="amrB"
                     /locus_tag="VGB88_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9647946.1"
                     /translation="MTSSTVRAAAVAGSFYPDHPEVLAAVVEDFLAEAADAPPHAPPK
                     AMIVPHAGYVYSGPVMARAYARLRDSGAAAGIRRVVLLGPSHYVALAGLAVPRASAFA
                     TPLGRIPLDGAAIAALAALPGVSINDAAHRDEHALEVQLPFLQRLLGAFEIVPLVAGR
                     AVPALVAGVLDALWGGPETLIVISSDLSHYHDQATARRL"
     gene            complement(720..1616)
                     /locus_tag="VGB88_10645"
     CDS             complement(720..1616)
                     /locus_tag="VGB88_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9647947.1"
                     /translation="MPHADSDGVKLYYVDCRADGAGADATPIIFVHEFAGDHRSWEPQ
                     IRYFSRFRRTIAYNARGYPPSDVPPDPKQYAQDIQADDVGHVLDHVGIERAHVVGLSM
                     GGFATLHFGLRHPHRARSLVVAGCGYGAEAKGRAEFQAGAEAMADVYLARGAAAVAED
                     YARAATRVQFENKDPRGWAEFKAQLAEHSAEGSANTMRGYQKTRPSLYDLEDQLKKLI
                     VPTLVVTGDEDEPCLDPDLFLKRTIPSAGLWVLPKCGHTINLEEPDAFNRGVADFIAK
                     VEAGAWGLRDPRSLSAPMLVRR"
     gene            complement(1648..2217)
                     /locus_tag="VGB88_10650"
     CDS             complement(1648..2217)
                     /locus_tag="VGB88_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647948.1"
                     /translation="MSAARRPRATLARALDAFAAGLDHASWFAAVGEPLTPSESADAA
                     GYLAGLGLAGARVAEVADWGAAGAAIRDSAWDRRWWDAVERERKLLLHAARKKHGGDP
                     VMAALSKATQAASDRVAGRAAEAAAARGVADPGLVKAAAGAGIESAYQAALALAAGAP
                     ADHPFRLRFSLFEAGHWPLALIAGRFDVF"
     gene            complement(2214..2924)
                     /locus_tag="VGB88_10655"
     CDS             complement(2214..2924)
                     /locus_tag="VGB88_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX9647949.1"
                     /translation="MSDIAPVKTAEDLRHQPQEYQDAVRKIVISHAVNELYGAQVFDE
                     PAIALAPTPYAKWLTCRVAMEEYGHHVRFKELGETIGVPSATMTPEHKQPLSIFAFPL
                     QTWPEFCVIKLLADLAEILQVEDLAQCSFHPLRNLARMTMPEERFHAQFGKDFCTELC
                     ETAAGRAAVQDAIDRYFPLLPAFFGRAKSKNNEMFRKWGIKQRTNEDMLADFVRRAKE
                     LVEDGLGLRLPEVELPAA"
     gene            complement(2957..3889)
                     /locus_tag="VGB88_10660"
     CDS             complement(2957..3889)
                     /locus_tag="VGB88_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9647950.1"
                     /translation="MSGALDGTVAIVTGGARGVGLAIARRLAGDGAQVVIADNGTGID
                     GRGADPEVAEAAAAAIGEAAAAYTETVARPEAATALVDLALRRFGRLDIVVNNAAILR
                     DAFVFKGAPEDWQAVIETNLSAAYYLIGAASGALREQAKADAAYDRGRIVNITSSAGL
                     YGNLGQAAYASAKAGLVGLTRVTAMDLARSRVACNAVAPFAHTRVTEIIRPANDAQAQ
                     YKERALRVAPDHVARFVAFLCSARAAGITGQLFAVRGREVFLFAQPRPVARIARHDED
                     WTVDALADAVGRELAPRFTDLSTDLEAFNTEPVV"
     gene            complement(3886..4929)
                     /locus_tag="VGB88_10665"
     CDS             complement(3886..4929)
                     /locus_tag="VGB88_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9647951.1"
                     /translation="MEPCHVPFNVTIRNLDRTIEVEPGETILEAAILAGVDYPCGCQS
                     GNCGACKSTLVSGEVEMSPYSDFALLPEERERGLILACRSVPWSDCAVAWLEQDEMVM
                     HASRVMTCRVAAIEALTDDVRRLKLDIESGGPFDFSPGQYARLTFGRLPGRDFSMANR
                     PDEAGLEFHVRLLPGGTVSRFIADSLAVGDKVRIDGPMGIAYLRDSHRGPILAIAGST
                     GLAPILSIVATALDKGMPQPIHLYFGVRDERDLYDMDRLEALADAHDNLTVVPVLSDP
                     VAPTERRTGLVTDAVARDFADLDGAKAYVAGPPAMVEAAVPLLVRLGVRRADCHYDAF
                     YTEAEKAAVGGAA"
     gene            5180..5656
                     /locus_tag="VGB88_10670"
     CDS             5180..5656
                     /locus_tag="VGB88_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011749744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9647952.1"
                     /translation="MARALRSVCRWLALAGGAVLVALTLLTVSSIVLREVAGRPIPGD
                     FELVELGCAIAIFAFLPYCQLIGGNVRVEFVTARWPRPVRAALDALASLAYAAIAALL
                     TWRLALGGADLRSFHETTMVLGVPVWLAFVAIVPSVALLALASAYTTWTGWRGTGG"
     gene            5653..6972
                     /locus_tag="VGB88_10675"
     CDS             5653..6972
                     /locus_tag="VGB88_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9647953.1"
                     /translation="MSGVEAGTGLFVLLLAMIALRVPIGLAMLLAGMIGYAGLNGWLP
                     LIAYLKTAPYYRFSTYELSVIPLFLLMGQFATQAGLSRALFESANRLFGRLRGGIAMA
                     AVGGCAAFGAICGSSLATAATMGQVALPELSRYRYSGALATGALAAGGTLGILIPPSV
                     VLVIYALLTEQSIGKMFMAAVVPGIVAAAGYIAAIAVYVRLRPGAAPPPAAGMLAVSR
                     ASVGMAIAVLVIFVVVIGGIYSGRFTPTEGAAVGAFLTGAMAIWPGRMGGRGLVASLL
                     GAAQASAMIFLILFGAEVFNAALALTQMPQQAARAVAESGWSPMLVLGAMLLLYLVFG
                     CVMDSLSMILLTIPVFFPVISALDFGLGAEETAIWFGVLALIVVEVGLITPPVGLNVF
                     IINGIAREVPITETYRGVMPFLATDAVRVALLVAFPAITLALPRLLE"
     gene            7038..>8023
                     /gene="gtdA"
                     /locus_tag="VGB88_10680"
     CDS             7038..>8023
                     /gene="gtdA"
                     /locus_tag="VGB88_10680"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HEX9647954.1"
                     /translation="MTTTETTTEAAGDAKGARRRYYDDIAPLHLAPLWESFARLITPS
                     PASPAEPCLWRYDEVRPWLLRAGELISAKEAERRVLILENPGLPGRASATHSLYAGLQ
                     LILPGEVAPSHRHAQSALRFIMEGEGAYTAVEGERAIMHRGDLILTPSWTWHDHGNDG
                     EAPTIWLDGLDIPLVQLLDASFLARYPDDSQPVSHAPGVSAARFGRNMRPFGHLAEGP
                     ASPVFSYAYAEWREALEVMRRDQEWDACHGIKMEFINPLDGGPVMPTISAFVQLLPKG
                     FASDPYRATDGTIYSVVDGEGEVVIGDEAFDVGQRDVFVVPSWTPVRFAAGT"
BASE COUNT         1054 a         2850 c         2933 g         1186 t
ORIGIN      
        1 tccagccgcc gcgcggtcgc ctggtcgtgg tagtgcgaga ggtccgagct gatcacaatc
       61 aaggtctccg gcccgcccca cagcgcatcg agcacgcccg cgaccagcgc cggcacggcg
      121 cggcccgcga ccagcggcac gatctcgaac gcgcccagca gccgttgcag gaacggcagc
      181 tggacctcga gtgcgtgctc gtcgcggtgg gcggcgtcgt tgatcgagac gcccggcagt
      241 gccgccagcg ccgcgatcgc cgcgccatcg agcgggatgc ggccgagcgg ggtggcgaac
      301 gcgctcgcgc gcggcaccgc aaggccggcc agcgccacgt aatgcgacgg tcctaacagc
      361 accacccggc ggatgcccgc cgccgcgccg ctgtcccgca gccgggcgta ggcgcgagcc
      421 atcaccggcc ccgagtagac gtagccggca tggggcacga tcatcgcctt gggcggggcg
      481 tgcggcgggg cgtccgcggc ctcggccaga aagtcctcga ccaccgcagc gagaacctcc
      541 ggatggtcgg gatagaagct gccggcgacc gccgcggccc ggaccgtgct cgaggtcatg
      601 gcttgcccgt tcgtctgcgc cgccccacgc agcccggcat atgcgcaccc cggcgctccg
      661 ccgcaaggct gtttcgcggc ccggccggct acgcgacgtt tggccaaacg cattgcgcgt
      721 cagcgcctga ccaacatcgg cgcgctcagc gagcgcgggt cgcgcaggcc ccaggcgccg
      781 gcctcgactt tggcgatgaa atcggcgacg ccccggttga aggcgtccgg ctcctcgagg
      841 ttgatggtgt ggccgcattt cggcagcacc cacagcccgg cgctcgggat ggtgcgcttg
      901 aggaacaggt cggggtcgag gcacggctca tcctcgtcgc cggtgacgac caaggtcggc
      961 acgatcagct tcttgagctg gtcctcgagg tcgtagagcg acggccgggt cttctggtag
     1021 ccgcgcatgg tgttggccga gccttcggcc gagtgctcgg cgagctgcgc cttgaactcg
     1081 gcccagccgc gcgggtcctt gttctcgaac tgaacccggg tcgcggcgcg cgcgtagtcc
     1141 tcggccaccg ccgcggcacc tctggcgagg tagacgtcgg ccatcgcctc ggcgccggcc
     1201 tggaactccg cgcgcccctt cgcctcggcg ccgtaaccgc agccggcaac caccagcgag
     1261 cgcgcgcggt gcggatgccg caggccgaaa tgaagggtgg cgaaaccgcc catcgacagc
     1321 ccgaccacgt gcgcccgctc gatgccgaca tggtcgagca cgtggccgac gtcgtcggcc
     1381 tggatgtctt gggcgtactg cttcgggtcg ggcggcacgt cggacggcgg gtagccgcgc
     1441 gcgttgtagg cgatggttcg gcggaagcgc gagaagtagc ggatctgcgg ctcccagctg
     1501 cggtgatcgc cggcgaactc gtggacgaag atgatcggcg tggcgtcggc gccggcgccg
     1561 tcggcgcggc agtcgacgta gtacagcttg accccgtcgg agtcggcatg aggcatgggc
     1621 ggtctcctcc ctggcgcggg tgcgcggtca gaacacgtcg aagcggccgg cgatcagcgc
     1681 cagcggccag tggccggcct cgaacaggct gaacctgagc cggaacggat ggtcggcggg
     1741 cgcgcccgcg gcgagcgcga gcgcggcctg gtaggcggac tcgatgccgg ccccggcagc
     1801 cgccttgacc aggcccggat cggcgacgcc gcgggccgcc gcggcttcgg cggcgcggcc
     1861 cgcgacccga tcggacgcgg cctgggtggc cttgctgagg gcggccatca ccgggtcgcc
     1921 gccgtgcttc ttcctggcgg cgtgcagcag cagcttgcgc tcgcgctcga ctgcgtccca
     1981 ccagcgccgg tcccaggcgg agtcgcggat cgcggccccg gccgcgcccc agtccgcaac
     2041 ctcggcgacg cgcgcgccgg ccaagccgag gccggcaaga tagccggccg cgtcggcgct
     2101 ctcggatggg gtcagcggct cgcccaccgc ggcgaaccac gaggcgtggt cgagaccggc
     2161 ggcgaacgca tcgagcgcgc gggcgagcgt cgcgcgcggg cggcgcgcgg cgctcacgcc
     2221 gcaggcagct cgacttcggg caggcgcagg ccgagcccgt cctcgaccag ctccttcgcc
     2281 cggcgcacga aatccgccag catgtcctcg ttggtgcgct gcttgattcc ccacttgcgg
     2341 aacatctcgt tgttcttcga cttcgcccgg ccgaagaagg cgggcagcag cgggaagtag
     2401 cggtcgatcg cgtcctggac cgcggcgcgg cccgcggcgg tctcgcacag ctcggtgcag
     2461 aagtccttgc cgaactgggc gtggaagcgc tcctcgggca tggtcatgcg ggcgaggttg
     2521 cgcagcgggt ggaacgagca ctgggcgagg tcctcgacct gaaggatctc ggcgaggtcg
     2581 gccaacagct tgatgacgca gaactcgggc caggtctgga gcgggaaggc gaagatcgac
     2641 agcggctgct tgtgctcggg cgtcatggtc gcggacggca cgccgatggt ctcgcccagc
     2701 tccttgaagc gcacgtggtg gccgtactcc tccatcgcca cccggcaggt cagccacttg
     2761 gcgtagggcg tcggcgcgag cgcgatcgcc ggctcgtcga acacctgggc gccgtacagc
     2821 tcgttgaccg cgtggctgat cacgatcttg cgcaccgcgt cctgatactc ctgcggctgg
     2881 tgccgcaggt cctcggcggt cttgaccggg gcgatgtcgc tcatggtcga ctctcctggt
     2941 tgtccttgga gcccgctcac acgacgggct cggtgttgaa tgcttcgagg tcggtcgaaa
     3001 ggtcggtgaa gcggggggcg agttcacgcc cgaccgcgtc ggcgagcgcg tcgacggtcc
     3061 agtcctcgtc atgcctcgcg atccgcgcca ccgggcgcgg ctgggcgaac aggaacacct
     3121 cgcggccgcg caccgcgaac agctggccgg tgatgccggc ggcacgcgcg ctgcacagga
     3181 aggcgacgaa gcgcgcgacg tggtcggggg ccacgcgcag cgcgcgctcc ttgtactgcg
     3241 cctgggcgtc gttggccggg cggatgatct cggtcacgcg ggtatgcgcg aacggcgcca
     3301 ccgcgttgca cgccacccgg ctgcgcgcga ggtccatcgc ggtaacgcgc gtcagcccca
     3361 cgagccccgc cttggcgctg gcataggccg cctggccgag attgccgtac agcccggcgc
     3421 tcgaggtgat attgacgatc cggccgcggt cgtaagccgc gtcggccttg gcctgctcgc
     3481 gcagtgcgcc cgacgccgcg ccgatcaggt agtaggcggc cgacaggttg gtctcgatca
     3541 ccgcctgcca gtcctcgggt gcgcccttga acacgaacgc atcgcgcagg atggcggcgt
     3601 tgttgaccac gatgtcgagc cgcccgaagc ggcgcagggc gagatcgacc agggcggtcg
     3661 cggcctcggg ccgggcgacg gtttcggtat acgccgccgc cgcctcgccg atcgcggccg
     3721 cagccgcctc ggcgacctcg ggatcggcgc cgcggccgtc gatgccggtg ccgttgtcgg
     3781 cgatcaccac ctgggcgccg tcgccggcga gcctgcgcgc gatcgcgagc ccgacgccgc
     3841 gggcgccgcc ggtgacgatg gcgaccgtgc cgtcgagcgc cccgctcatg ccgcgcctcc
     3901 gaccgccgcc ttctcggcct cggtgtagaa cgcgtcgtag tggcagtcgg cgcggcgcac
     3961 gccgaggcgc accagcagcg gcaccgccgc ctcgaccatc gccggcggcc cggcgacata
     4021 ggccttggcg ccgtcgaggt cggcgaagtc gcgcgccacc gcgtcggtaa ccaggcccgt
     4081 gcgccgctcg gtcggcgcga ccgggtcgga cagcaccggc accacggtga gattgtcgtg
     4141 cgcgtcggcc agcgcctcga gccggtccat gtcgtacagg tcgcgctcgt cgcggacgcc
     4201 gaaatagagg tggatcggct gcggcatgcc tttatccaat gcggtggcga cgatcgacag
     4261 gatcggcgcg aggccggtcg agccggcgat cgccaggatc gggccgcgat gggagtcgcg
     4321 caaataagca atgcccatcg gcccgtcgat gcgcaccttg tcgccgaccg cgagcgagtc
     4381 ggcgatgaag cggctgaccg tgccgcccgg cagcaggcgg acatggaact cgagcccggc
     4441 ctcgtccggc cggttggcca tcgagaagtc gcgccccggc agccggccga aggtgaggcg
     4501 cgcatattgg ccgggcgaga agtcgaacgg cccgcccgac tcgatgtcga gcttaagccg
     4561 gcggacgtcg tcggtgaggg cctcgatcgc agccacgcgg caggtcatca cccggctcgc
     4621 gtgcatcacc atctcgtcct gctcgagcca cgccaccgcg cagtcgctcc acggcaccga
     4681 ccggcaggcg aggatcaggc cgcgctcgcg ctcctcgggc agcagcgcga agtcagagta
     4741 cgggctcatc tcgacctcgc ccgagaccag tgtcgacttg cacgcgccgc aattgcccga
     4801 ctggcagccg cacgggtaat ccacgccggc caggatcgcc gcttcgagaa tggtctcgcc
     4861 gggctccacc tcgatcgtcc ggtccaggtt cctgatcgtg acgttgaacg gcacgtggca
     4921 cggctccacg gtttgcgccc gcggcgccgc gggcgcgcca acaataggcg cgggcaagtc
     4981 aaaatcaagg accggcccgg cgctgcggcc gctcgcggcc cggcgttttt gacagggcgg
     5041 gcgcggcccg cctatcaatg cgccaagacg gctgccatga tgcggccgcc gcggacggca
     5101 gcggagggac ggcgatgacg cgggcgacgg gcgcggcgcg atttggcgcg aggtcgccgg
     5161 cgtcggggcc gcggtgagcg tggcgcgcgc gctccggtcg gtttgccgct ggctcgcgct
     5221 cgccggcggc gcggtgctgg tcgcgctgac cctgctcacg gtctcgagca tcgtgctgcg
     5281 cgaggtcgcg ggccggccga tcccgggcga cttcgagctc gtcgagctcg gctgcgcgat
     5341 cgcgatcttc gccttcctgc cctattgcca gctgatcggc ggcaacgtcc gggtggagtt
     5401 cgtcaccgcc cgctggccgc ggccggtcag ggccgcgctc gatgccctgg cgagcctcgc
     5461 ctatgccgcc atcgccgcgc tgctcacctg gcggctcgcg ctcggcggcg ccgacctgcg
     5521 cagcttccac gagaccacga tggtcctcgg cgtgccggtc tggctcgcct tcgtcgcgat
     5581 cgtcccctcg gtggcgctgc tcgcgctggc ctcggcctac accacctgga ccggctggcg
     5641 ggggaccggc gggtgagcgg tgtcgaggcc ggcaccggcc tgttcgtgct gctgctggcg
     5701 atgatcgcgc tccgggtgcc gatcgggctc gccatgctgc ttgccggcat gatcggctat
     5761 gccgggctga acggctggct gccgctgatc gcctatctca agaccgcacc ctactaccgc
     5821 ttctcgacct acgagctgtc ggtgatcccg ctgttcctgc tgatgggcca gttcgccacc
     5881 caggccgggc tcagccgggc gctgttcgag tccgccaacc ggctgttcgg gcgcctgcgc
     5941 ggcggcatcg ccatggccgc ggtcgggggc tgcgcggcgt tcggcgcgat ctgcggctcc
     6001 tcgctcgcca ccgccgcgac catgggccag gtggcgctgc ccgagctcag ccgctaccgc
     6061 tactcgggcg cgctcgccac cggcgcgctc gccgccggcg gcacgctcgg catcctgatc
     6121 ccgccgtcgg tggtgctggt gatctacgcg ctgctcaccg agcagagcat cggcaagatg
     6181 ttcatggccg cggtggtgcc gggcatcgtc gccgccgccg gctatatcgc ggcgatcgcg
     6241 gtctatgtgc ggctccgccc cggggccgcg ccgccgccgg cggcggggat gctcgcggta
     6301 agcagggcga gcgtcggcat ggcgatcgcg gtgctggtga tcttcgtggt ggtgatcggc
     6361 ggcatctata gcggccgctt tacgcccacc gagggcgccg cggtgggcgc gttcctgacc
     6421 ggcgcgatgg cgatctggcc ggggcggatg ggcgggcgcg gcctggtcgc cagcctgctc
     6481 ggcgccgccc aggcgagcgc catgatcttc ctgatcctgt tcggcgccga ggtgttcaac
     6541 gccgcgctcg cgctcaccca gatgccgcag caggcggcgc gcgcggtggc cgagtcgggc
     6601 tggtcgccga tgctggtgct gggcgccatg ctgctcttgt acctggtgtt cggctgcgtc
     6661 atggactcgc tgtcgatgat cctgctgacc atcccggtgt tcttcccggt gatctcggcg
     6721 ctcgatttcg ggctcggcgc ggaggagacc gcgatctggt tcggcgtgct cgccctgatc
     6781 gtggtcgagg tcgggctgat cacgccgccg gtcgggctca acgtgttcat catcaacggc
     6841 atcgcgcgcg aggtgccgat caccgagacc tatcgcgggg tgatgccgtt tctcgccacc
     6901 gatgcggtgc gggtggcgct gctggtcgcg ttcccggcga tcacgctggc gctgccgcgc
     6961 ctgctcgagt gacacgtgcg cctggacgcc cgccggcgcg gaccttagat ttccttcgcc
     7021 cggaggagga ccggatcatg accacgaccg agaccacgac cgaggccgcg ggcgacgcca
     7081 agggggcgcg gcggcgctac tacgacgaca tcgcgccgct ccatctggcg ccgctgtggg
     7141 agtcgttcgc gcgcctgatc acgccgagcc cggcgagccc ggccgagccg tgcctgtggc
     7201 gctacgacga ggtgcgcccg tggctgctga gagcgggcga actgatcagc gccaaggagg
     7261 ccgagcggcg ggtgctgatc ctcgagaacc cgggcctgcc ggggcgcgcc agcgccaccc
     7321 actcgctcta tgccggcctg cagctgatcc tgccgggcga ggtcgcgccc agccaccgcc
     7381 acgcccagtc ggcgctgcgc ttcatcatgg agggcgaggg cgcctacacc gcggtcgagg
     7441 gcgagcgcgc catcatgcac cgcggcgacc tgatcctgac cccgtcctgg acctggcacg
     7501 accacggcaa tgacggcgag gcgccgacca tctggctcga cggcctcgac atcccgctgg
     7561 tgcagttgct cgacgcgagc ttcctcgcgc gctacccgga cgacagccag ccggtctcgc
     7621 acgcgcccgg cgtgagcgcg gcgcgcttcg ggcgcaacat gcgcccgttc ggccacctgg
     7681 ccgagggacc ggcgtcgccg gtgttcagct acgcttacgc cgagtggcgc gaggcgctcg
     7741 aagtgatgcg ccgcgaccag gaatgggatg cttgccacgg catcaagatg gagttcatca
     7801 acccgctcga cggcggcccg gtgatgccca ccatctcggc gttcgtgcag ctgttgccga
     7861 agggcttcgc cagcgatccc taccgcgcga ccgacggcac catctacagc gtggtcgacg
     7921 gcgagggcga ggtggtgatc ggcgacgagg ccttcgatgt cggccagcgc gacgtgttcg
     7981 tggtgccgag ctggaccccg gtgcgcttcg ccgccggcac gga
//
LOCUS       DASWHU010000288         6983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1655006, whole genome shotgun sequence.
ACCESSION   DASWHU010000288 DASWHU010000000
VERSION     DASWHU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655006"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VGB88_10685"
     CDS             complement(<1..725)
                     /locus_tag="VGB88_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007112710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9647955.1"
                     /translation="MTTVKAGLIQMALKADTAETPAVIREAMIAAHVPFIERAGAAGV
                     QVLCLQEIFTQPYFCPSQDPKWYAAVEKVPDGPTVRLMQDFARKHAMVIVVPIYEQEM
                     IGVYYNTSAVIDADGRYLGKYRKTHIPQVAGFYEKFFFKGGNSGWPVFETAYCKLAVY
                     TCYDRHFPEGWRALALNGAEYIVNPSATVAGLSQYLWQLEQPAAAVANGVFIGAINRV
                     GEEAPWNIGRFYGSSYVVDPRGK"
     gene            complement(749..2005)
                     /locus_tag="VGB88_10690"
     CDS             complement(749..2005)
                     /locus_tag="VGB88_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX9647956.1"
                     /translation="MRQDLSAAAAAAVNEDRLWRHHMDIARIGATGRGGVNRQALTEH
                     DAEARKRVLEWAGARGFAASVDAIGNLFIRRPGTDPKASPVVSGSHLDTQPAGGNFDG
                     VFGVLAALEVLEAAADAGIKTRRPLEAVVWTNEEGARFQPTTMGSSVFAGAVPLERAL
                     ATTDRDGVTVAAALAETLARLRVGDRRDFRFPMAGYLEAHIEQGPVLEATGKTIGIVT
                     GIQGLRWFRIEVTGEEAHAGTTPLKRRKDALVAAVAMVGALQEHMRDDADVVRFTVGR
                     FEVSPNSPNTVPGRVLFTIDFRHPEAAVLRRLGDRIEAVCRDHAGGCEVSVAETLDSP
                     PTVFDPEILGVLRDAAARQGLAHMDIMSGATHDAKYMAGLYPSGMLFVPCAGGVSHNE
                     AESAASADLAAGARVLAEAMIALANR"
     gene            complement(1990..3399)
                     /gene="hydA"
                     /locus_tag="VGB88_10695"
     CDS             complement(1990..3399)
                     /gene="hydA"
                     /locus_tag="VGB88_10695"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX9647957.1"
                     /translation="MATVLIKGGRIVTAVDDYQADILIENGRIHTIGRDLAAGPGVEV
                     HDAAGLIAVPGGVDVHTHLDWDFGVARTVDTFATGTMAAAFGGTTTVVDFCNQNRGES
                     PLKGLEDWHKRAASAAVDVGAHMIMLDVTDQSLADMKTLMAREGVTSFKLFMAYPGVL
                     MIDDAGLFKAMRIAGSNGGMICVHAENGLVIQVLVEEAVKRGHTAPKYHMLTRPASME
                     GEATHRAIALSELAEAPLYVVHLSAAEALHAVTEARDRGVPVHAETCPHYLFLTGEDY
                     ERPEFEGAKYVMTPPLRDHSHQAALWRGLATSDLQVVSTDHCPFCFNEQPYGIKYSKQ
                     QGKDDFSKIPNGAPGIETRLPLVFDGAVRARGMSLNRFVGLTSTTPAKLFGLFPRKGT
                     IAVGSDADIVLFDANESWTIRAAEQHSRVDYTLFEGRQVTGRVKKVFLRGRLIVDGPE
                     WKGREGMGEFVRRGECGKI"
     gene            3590..4075
                     /locus_tag="VGB88_10700"
     CDS             3590..4075
                     /locus_tag="VGB88_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9647958.1"
                     /translation="MAEVGHVALDGGCTCGAVRYRLKARPLFVHCCHCTWCQRETGSA
                     FAVNALIEASNVELPRGSVVQTTLPSASGKGQVFWRCPDCGIALWSNYAGAGPGVHFI
                     RAGTLDDPSLAPPDIHIYTSAKQPWVVLPEGVPAVEEFYRPSAYWPVQSIERYKAARA
                     S"
     gene            complement(4189..5952)
                     /locus_tag="VGB88_10705"
     CDS             complement(4189..5952)
                     /locus_tag="VGB88_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9647959.1"
                     /translation="MAGARRRKFLIVLIKPSHYDDDGYVIQWLRSAIPSNTLAALNGL
                     VVDCIERKVLGEDVEVTVAAFDETNTRIRPQAIIRRFQDSGGAGLVALVGVQSNQFPR
                     AVDIARPLCAAGIPVCIGGFHVSGCLAMLPELPADIQAAMALGISMFAGEAEGRLDEV
                     LLDAQAGSLKPLYNYMDDLPSIAGTPPPILPATRVMRVAGGITSFDAGRGCPFQCSFC
                     TIINVQGRKSRRRSPDDVERIIRDNAAQGIHSFFITDDNFARNKDWESIFDRLIQLRH
                     EAKLPIRFIIQVDTLCHKIPHFIEKAARAGVRRVFIGLENINPESLLGVQKRQNRITD
                     YRQMLQAWKDVGVITYAGYILGFPNDTPDSIVRDIEIIKRELPIDLLEFFYLTPLPGS
                     ADHKALWDKGAWMDPDMNKYDLNHVTTAHPRMSEAELRRAYRLAWKTYFTIEHIETVM
                     RRAVARRISAGRIMFLMLWFYGCITFEKLHPLEGGYLRRKYRRDRRPGLPIESPLVFY
                     PRYAGETLYKHARLFHLVWRLAKIRRRLKRDPEARSYTDLALRPVREEELDELGIFTA
                     SAAAKSAVDRARRQRQAAKAL"
     gene            complement(6218..>6983)
                     /locus_tag="VGB88_10710"
     CDS             complement(6218..>6983)
                     /locus_tag="VGB88_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9647960.1"
                     /translation="FDRLAIETTGLADPAPIVFTLMAEPVVRHHFRLGNILATVDAVN
                     GRAHLARNPESVKQVAVADRIIITKTDIADAADVAALRATLHRFNPTAQVIDGARDRA
                     AADDLLTGDVYDPKTKAAEVSRWLAHEADEAESQPAHAHDVSRHARDIRSFCVTIDRP
                     LDWTAFGIWLTMLLHAHGPDVLRVKGLLNVAGSPAPVVIHGVQHVIHPPAHLDAWPDA
                     DRRSRIVFIVRGLEPARIERSLAAFNRLANPARRAA"
BASE COUNT         1016 a         2381 c         2465 g         1121 t
ORIGIN      
        1 atcttgccgc gcgggtcgac cacgtaggac gagccgtaga agcggccgat gttccacggc
       61 gcctcctcgc cgacccggtt gatcgcgccg atgaacacgc cgttggcgac cgccgccgcc
      121 ggctgctcga gctgccacaa atactggctc aatccggcca cggtcgccga ggggttgacg
      181 atgtactcgg cgccattcag cgccagcgcc cgccagccct cggggaagtg gcggtcgtag
      241 caggtgtaga ccgcgagctt gcagtaggcg gtctcgaaca ccggccagcc gctgttgccg
      301 cccttgaaga agaacttctc gtagaagccg gcgacctgcg ggatgtgggt cttgcggtac
      361 ttgccgagat agcggccgtc ggcgtcgatc accgccgagg tgttgtagta gaccccgatc
      421 atctcctgct cgtagatcgg caccacgatg accatcgcgt gcttcctggc gaagtcctgc
      481 atcaggcgca cggtcgggcc atcggggacc ttctcgaccg cagcgtacca tttggggtcc
      541 tggctcgggc agaaataggg ctgggtgaag atctcctgga ggcacagcac ctgcaccccg
      601 gcggcgccgg cccgctcgat gaacgggacg tgcgccgcga tcatcgcctc gcgaattacc
      661 gcgggcgtct cggccgtgtc ggccttcagc gccatttgga tcagtcccgc cttcaccgtc
      721 gtcatgccgg cctcctgccg ccgcgcggtc aacgattggc cagcgcgatc attgcctcgg
      781 caagcacccg cgcgccggcg gcgaggtcgg ccgaggccgc gctctcggcc tcgttgtggc
      841 tgacgccgcc cgcacacggc acgaacagca tgccgctcgg gtacaggccg gccatgtact
      901 tggcgtcgtg ggtggcgccc gacatgatgt ccatgtgggc gaggccctgg cgcgccgcgg
      961 cgtcgcgcag cacgcccaga atctccgggt cgaagaccgt cggcggggaa tccagcgtct
     1021 cggccacgct gacctcgcag cccccggcgt ggtcgcggca gaccgcctcg atgcgatcgc
     1081 ccaggcgcct gagcaccgcc gcctcgggat ggcggaagtc gatcgtgaac agcacccgcc
     1141 cgggcaccgt gttgggcgag ttgggcgaca cctcgaagcg cccgaccgtg aaccggacca
     1201 cgtcggcgtc gtcgcgcata tgctcctgca acgcgcccac catggcgacc gcggcgacca
     1261 gcgcgtcctt gcgccgctta agcggcgtcg tacccgcatg cgcctcctcg ccggtgacct
     1321 cgatgcggaa ccagcgcagg ccctggatgc cggtgacgat gccgatggtc ttgccggtcg
     1381 cctccagcac cgggccttgc tcgatatgcg cctccagata gcccgccatc gggaagcgga
     1441 agtcgcgccg gtcgccgacg cgtaagcgcg cgagcgtctc ggcgagggcg gcggcgaccg
     1501 tgaccccgtc gcggtcggtg gtggcgagcg cgcgctcgag cggcaccgcg cccgcgaaca
     1561 cgctcgagcc catggtcgtc ggctggaagc gcgcgccttc ctcgttggtc cacaccaccg
     1621 cttcgagcgg gcgcctggtc tttatgccgg cgtcggcggc cgcctccagc acctcgagcg
     1681 cggcgagcac gccgaacacg ccgtcgaagt tgccgccggc cggctgggtg tcgaggtgcg
     1741 agccggagac caccgggctc gccttgggat ctgtgcccgg gcggcggatg aacaggttgc
     1801 cgatcgcatc gacgctggcg gcgaagccgc gcgcgcccgc ccattccagc acccgcttgc
     1861 gcgcctcggc gtcatgctcg gtcagcgcct ggcggttgac gccgccgcgg ccggtggcgc
     1921 cgatccgggc gatgtccatg tggtggcgcc acagccggtc ctcgttgacc gctgctgcgg
     1981 cggcggcgct cagatcttgc cgcattcgcc gcgcctcacg aactcgccca tgccctcgcg
     2041 gcccttccac tcaggcccgt cgacgatcag ccggccgcgc aggaacacct tcttcacccg
     2101 cccggtcact tggcggccct cgaacagggt gtagtcgacg cggctgtgct gctcggcggc
     2161 gcggatggtc cagctctcgt tcgcgtcgaa caggacgatg tcggcgtccg agccgaccgc
     2221 gatcgtgccc ttgcgcggga acagcccgaa cagcttggcg ggcgtggtcg aggtgaggcc
     2281 gacgaagcgg ttgagcgaca tgccgcgcgc gcgcacggcg ccgtcgaaca ccagcggcag
     2341 gcgggtctcg attcccggcg cgccgttggg gatcttgctg aagtcgtcct tgccctgctg
     2401 cttcgagtac ttgatgccgt aaggctgctc gttgaagcag aacgggcagt ggtcggtgga
     2461 caccacctgc aggtcggagg tcgcgagccc gcgccacagc gccgcctggt ggctgtggtc
     2521 gcgaagcggc ggggtcatca cgtacttggc gccctcgaac tccgggcgct cgtagtcctc
     2581 gccggtcagg aacaggtagt gcgggcaggt ctcggcgtgc acaggcacgc cgcgatcgcg
     2641 cgcctcggtc accgcatgaa gcgcctcggc cgccgacagg tggacgacgt agagcggcgc
     2701 ctcggcgagc tccgacagcg cgatggcgcg gtgggtcgcc tcgccctcca tcgaggcggg
     2761 gcgggtgagc atgtggtact tgggcgccgt gtggcctcgc ttgaccgcct cctcgaccag
     2821 cacctggatc accaggccgt tctcggcatg gacgcagatc atgccgccgt tgctgcctgc
     2881 gatgcgcatc gccttgaaca gcccggcgtc gtcgatcatc agcacgcccg gataggccat
     2941 gaacagcttg aagctggtca ccccctcgcg cgccatcagc gtcttcatgt cggcgaggct
     3001 ctggtcggtg acgtcgagca taatcatgtg cgcgccgacg tcgaccgcgg cgctggcggc
     3061 gcgcttgtgc cagtcctcga ggcccttgag cgggctctcg ccgcggttct ggttgcagaa
     3121 gtcgaccaca gtggtggtgc cgccgaacgc cgccgccatg gtgccggtgg cgaaggtatc
     3181 gacggtgcgc gcgacgccga agtcccaatc gaggtgagtg tgcacgtcga ccccgccggg
     3241 caccgcgatc agcccggcgg cgtcgtggac ctcgacgccc gggcccgccg cgaggtcgcg
     3301 gccgatggtg tggatgcgcc cgttctcgat caggatgtcg gcctggtagt cgtcgaccgc
     3361 ggtcacgatc cgcccgccct tgatcagcac cgtcgccatc gccgcctccc cgtctcgatg
     3421 ccgccgcagt ctaggacggc gggcgacggc cgcgcaacgc cgggcgggga aattgccgcg
     3481 accgcccgct gccgctgcgc ccgatcaccc gaggcgacgc cgccattcgt cttgcttgcg
     3541 atcgtcggcc gcccgaaagt ggcgcgatcg ggccgtggag ggaagcgcga tggcggaggt
     3601 cgggcacgtg gcgttggacg gcggttgcac ctgcggcgcg gtgcgctatc gcctgaaagc
     3661 gcggccactg ttcgttcatt gctgccactg cacctggtgc cagcgcgaaa ccggatcggc
     3721 gttcgcggtg aacgccttga tcgaggcgtc caacgtcgag ctcccgaggg gcagcgtggt
     3781 gcagacgact ctgccctccg ccagcggcaa gggccaggtg ttctggcgct gccccgactg
     3841 cgggatagcg ttgtggagca actacgccgg cgccggtccg ggcgtgcatt tcatccgggc
     3901 gggaacgctc gacgatccct cgctcgcacc gccggacatc cacatttata cgtccgccaa
     3961 gcagccatgg gtggtcctgc cggagggcgt tccggccgtc gaagagtttt atcggccgtc
     4021 cgcttactgg ccggtgcaga gcatcgaacg ctacaaggcc gcaagagcgt cctaatccgg
     4081 acccggccac gtcgtgcggg ggcacgcgag tccgattggg ccgcgatggg cgacgtgccc
     4141 gacacgtctc acccattcaa gctcgcgagc ggctcggttc ggcagtagct aaagagcctt
     4201 cgctgcctgg cgctggcggc gcgcccgatc gaccgcggac ttcgcggctg ccgaggcggt
     4261 gaagatgccg agctcgtcga gttcttcctc gcgcaccggg cggagcgcga ggtcggtgta
     4321 gctgcgcgcc tcgggatcgc gcttcagccg gcgcctgatc ttcgccagcc gccagaccag
     4381 gtggaacagc cgcgcgtgct tgtagagggt ctcgccggca tagcgcggat agaacaccaa
     4441 cgggctctcg atcggcagcc cgggccgccg gtcgcggcgg tacttgcggc gcagataccc
     4501 gccttcgagc ggatgcagct tctcgaaggt gatgcagccg tagaaccaca gcatcaggaa
     4561 catgatccgg ccagcgctga tgcgccgcgc caccgcccgc cgcatgacag tctcgatgtg
     4621 ctcgatcgtg aagtaggtct tccaggccag ccgataggcg cgccgcaact cggcctcgga
     4681 catcctggga tgggcggtcg tgacgtggtt caaatcgtac ttgttcatgt cggggtccat
     4741 ccacgcgccc ttgtcccaca gcgccttgtg gtcggcggag cccggcagcg gcgtcaggta
     4801 gaagaactcg agcaggtcga tcggcagctc gcgtttgatg atctcgatgt cgcgcacgat
     4861 cgagtccggc gtgtcgtttg ggaagccgag gatgtagccg gcataggtga ttacgccgac
     4921 gtctttccag gcctgcagca tttggcggta gtcggtgatc cggttctggc gcttctggac
     4981 gccgagcagg ctctcggggt tgatgttctc aagcccgatg aacacccgcc gcacgcccgc
     5041 cctcgccgcc ttctcgatga aatgcggaat cttgtggcac aacgtgtcga cctggatgat
     5101 gaaacggatc ggcagcttcg cctcgtggcg gagctggatc aggcggtcga atatgctctc
     5161 ccagtccttg ttgcgcgcga agttgtcgtc ggtgatgaag aagctgtgga tgccctgggc
     5221 ggcgttgtcg cggatgatgc gctcgacgtc gtcgggcgag cgccggcgcg acttccgccc
     5281 ctggacgttg atgatggtgc agaacgagca ctggaacggg cagccccgcc cggcatcgaa
     5341 gctggtgatg ccgccggcaa cccgcatgac ccgcgtcgcg ggcaggatcg gcggcggcgt
     5401 gccggcgatg ctcggcaggt cgtccatgta gttgtaaagc ggcttcagcg agccggcctg
     5461 ggcatcgagc aggacctcgt cgaggcgccc ctccgcctcg ccggcgaaca tcgagatgcc
     5521 gagcgccatc gccgcctgga tgtcggccgg cagctccggc agcatcgcca ggcagcccga
     5581 gacgtggaag ccgccgatgc agaccgggat gccggccgcg cagagcggcc gcgcgatgtc
     5641 gaccgcgcgc gggaactggt tggactggac accgaccagg gcgaccaggc ccgcgccgcc
     5701 tgaatcctgg aagcggcgga tgatcgcctg cggccggatg cgcgtgttgg tctcgtcgaa
     5761 cgcggcgacc gtgacctcga cgtcttcgcc gagcaccttg cgctcgatgc agtcgaccac
     5821 caggccgttg agcgccgcca gcgtgttcga cggaatcgcc gagcgcagcc attggatgac
     5881 gtaaccgtcg tcgtcgtagt gcgacggctt gatcaggacg atcaggaact tgcgccggcg
     5941 cgcgccggcc acggccggcg ccgggcgatg cgggcgcacg ccttcgccgc cccggtcggc
     6001 tcgcgcaggc ccgtgcgcga cgaacgcgac ccttgccgac tcatccaccg cggtccccca
     6061 ctcgctccga tcggcgccgt gatcgcggct cggccgtctt ccgcgcgatc agcggagcag
     6121 tgcgcccgag actgccgcca tattcggcca ttggcaaggc ccaagcctgg ccaagcagaa
     6181 ccagacgact cggcttcgcg acttggctcg gccgcgctca ggcggcgcgg cgggcggggt
     6241 tggcgagacg gttgaaggcg gcgagcgagc gctcgatgcg cgccggctcg aggccgcgga
     6301 cgatgaacac gatgcgcgag cggcggtcgg cgtcgggcca ggcgtcgaga tgggccggcg
     6361 ggtggatcac gtgctggacg ccgtggatca ccaccggcgc cggactgccg gcgacattca
     6421 gcagcccctt caccctgagc acgtccggcc cgtgagcatg gagcagcatg gtcagccaga
     6481 tgccgaacgc ggtccagtcg agcggccggt cgatggtgac gcagaagctc cggatgtcgc
     6541 gggcgtggcg gctgacgtcg tgggcgtggg cgggctggct ttcggcctcg tcggcctcgt
     6601 gcgcgagcca gcggctcacc tcggccgcct tggtcttcgg gtcgtagacg tcgccggtca
     6661 gcaggtcgtc cgccgcggcg cggtcgcggg cgccgtcgat cacttgcgcg gtcgggttga
     6721 accggtgcag cgtcgcgcgc agcgcggcca cgtcagccgc atcggcgatg tcggtcttgg
     6781 taataataat ccggtcggcg accgccacct gcttgacgga ttcggggttg cgtgcgagat
     6841 gtgcgcggcc gttgaccgca tcgaccgtgg ccaggatgtt gccgagccgg aagtggtggc
     6901 gcaccaccgg ctcggccatc agggtgaaca cgatcggcgc cgggtcggca agcccggtgg
     6961 tctcgatcgc caggcggtcg aag
//
LOCUS       DASWHU010000289         8798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2400588, whole genome shotgun sequence.
ACCESSION   DASWHU010000289 DASWHU010000000
VERSION     DASWHU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400588"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1310
                     /gene="purB"
                     /locus_tag="VGB88_10715"
     CDS             3..1310
                     /gene="purB"
                     /locus_tag="VGB88_10715"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9647961.1"
                     /translation="MPRYSRPEMAAIWAPETRFRIWLDIERFACEAQEQLGVIPRGVA
                     KALAERGRFEVARIAEIEAEVKHDVIAFLTNLAEHVGPEARFVHQGMTSSDVLDTCLA
                     VQLTRAADILLDDLDALLAVLERRAREHKLTPCIGRSHGIHAEPTTFGLKLAQAYAEF
                     ARNRDRLVAARAEVATCAISGAVGTFADIDPRVEAHVAAKLGLSPEPISTQVIPRDRH
                     AMFFATLGVIAASVERLATEVRHLQRTEVGEAEEYFAPGQKGSSAMPHKRNPVLSENL
                     TGLARLVRATVAPALEDVALWHERDISHSSVERAIAPDATVTLDFALARLAGVIDTLV
                     VYPEAMRRNLDLLGGLIHSQRVLLALTGTGMSREDAYRAVQRNAMRALQGDGDFKALL
                     VADKEVAARLDAKALDALFDLDHHLAHVETIFARVFGPGDGAG"
     gene            1451..1681
                     /locus_tag="VGB88_10720"
     CDS             1451..1681
                     /locus_tag="VGB88_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9647962.1"
                     /translation="MPITRAFKSGNSQAVRIPAELAYDDMTIELQITRLGDVITIAPA
                     RQSLKDAVAALRRLPRPPAVEIREPIEVPVRE"
     gene            1703..2101
                     /locus_tag="VGB88_10725"
     CDS             1703..2101
                     /locus_tag="VGB88_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9647963.1"
                     /translation="MAYLIDTNIAIHARDGTDSVLDKLAEHDGMVLLSALSLAELQRG
                     IYRDPAWTALRQARLEILLRHIPVLPFDAAAAIAYGRIIAECGWVKARDYDRMIAAHA
                     ISTSSVLVTDNEADFRDISLLTIENWAIPR"
     gene            complement(2151..2879)
                     /locus_tag="VGB88_10730"
     CDS             complement(2151..2879)
                     /locus_tag="VGB88_10730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9647964.1"
                     /translation="MAGLIAVNALVFLMVWSLPPQGVAAVIGDYALIPLRYTEPAWAR
                     AKGLDPTDYAPFLTNTFLHGGWLHVILNMWTLWIFGCAVEDRLGAARFTLLYFASGII
                     ASASHFAFNAASPVPALGASGAIAGVIGAYAVRFPRARVVLLVPVLFLPLLFQVPALA
                     FAAIWFALQVLQGAQEILAPAMAGGIAWWAHVGGFLAGVGLILVLRPPPGRGPRSPLQ
                     PPPPPGWRIRRRTRVPAVRGPWER"
     gene            2998..3657
                     /locus_tag="VGB88_10735"
     CDS             2998..3657
                     /locus_tag="VGB88_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647965.1"
                     /translation="MRAFCLAFMLALALVMPDARTATLVETSIAGESVWVIDDPLSKR
                     VLVASRAGSRLIDLDTETVYLIAPNGSTQKVEVSALPAVTATSSFEIEKVGPGPRIAD
                     YPTTRFRLSAEGRTCSLIDANLGLGEALERAMRAFDLMERLQSALGGADRGPCQRIPL
                     GEYARIGWGLRVADAGAPVVEVVSVVRNYEPLAEELALPTSAVDITMVVRDGGAPATT
                     R"
     gene            complement(3665..3985)
                     /locus_tag="VGB88_10740"
     CDS             complement(3665..3985)
                     /locus_tag="VGB88_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX9647966.1"
                     /translation="MTTFDNREREAEKKFEHDEELRFKAMARRNKLLGLWAADKLGLE
                     GDAAQAYAREVVLADLERPGDDDVVEKVLKDFEGRGVPLDAHKIRLEMDRLLPEARRQ
                     IMSE"
     gene            4146..4937
                     /locus_tag="VGB88_10745"
     CDS             4146..4937
                     /locus_tag="VGB88_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX9647967.1"
                     /translation="MCTLVILRRSGDSWPLILAANRDEVLARPWAPPARHWPERPHVV
                     AGRDLEAGGTWLGLGDGGVAAGVLNRPGALGPAPGKASRGELVLRALDHDSAAAAVRA
                     LRRLDATQFRPFNLVVADAGGAWWLRWAGAAGGIEAAPVPEGLSMITAHDLNDTASAR
                     IRRQLPRFRAAPEPDPALGDWSAWQRLLADSDGGADGPHSAMTLGPASGFGTVSSSLI
                     ALPARAGTAERARWLFAPGRPDTTEFEPVDLSPNQMLEGKYSSGR"
     gene            5093..5857
                     /gene="purC"
                     /locus_tag="VGB88_10750"
     CDS             5093..5857
                     /gene="purC"
                     /locus_tag="VGB88_10750"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9647968.1"
                     /translation="MARRRQIYEGKAKVLFEGPEPGTLVQYFKDDATAFNNQKRGTIT
                     GKGVLNNRISEYLMTRLSEIGIPTHFVRRLNMREQLIKEVEIIPIEVVVRNVAAGSLS
                     TRFGLAEGTSLPRSVVEYYYKSDELNDPMVSEEHITAFGWATPSELDEMLSMSLRVND
                     FLSGLFFALGIRLVDFKVEYGRLFEGDDMRIVLADEISPDNCRLWDIKTNEKLDKDRF
                     RRDLGRVEEGYQEVARRLGILPESGPRDLQGPDTMQ"
     gene            5869..6108
                     /gene="purS"
                     /locus_tag="VGB88_10755"
     CDS             5869..6108
                     /gene="purS"
                     /locus_tag="VGB88_10755"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9647969.1"
                     /translation="MKAKVHITLKEGVLDPQGKAIEHALKMLGFAGVDQVRQGKYLEI
                     ELAETDPAKALKAVEGMCARLLANAVIEDYQIEVS"
     gene            6152..6847
                     /gene="purQ"
                     /locus_tag="VGB88_10760"
     CDS             6152..6847
                     /gene="purQ"
                     /locus_tag="VGB88_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9647970.1"
                     /translation="MDAAVVVFPGSNCDRDVAVALTRACGRAPAMVWHRETELPAADL
                     IVLPGGFSYGDYLRPGAMAAQSPVMREVVARAQAGVAVLGICNGFQVLTECGLLPGVL
                     MRNRGLRFLCREAPLAVEAAEPPFTAGYRAGERIRLPIAHNDGNFFADEDTLDRLEGE
                     GLVALRFVAGPGAAEGANGPAAANGACRDIAGIVNARRNVLGLMPHPERACEPAHGNT
                     DGARLFAGLASSL"
     gene            6844..>8798
                     /gene="purL"
                     /locus_tag="VGB88_10765"
     CDS             6844..>8798
                     /gene="purL"
                     /locus_tag="VGB88_10765"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9647971.1"
                     /translation="MSGRAQTAEVTPEIAAAHGLDAAEYADIRHILGRVPSLTELGIF
                     SAMWSEHCSYKSSRKWLARLPTAAPWVIEGPGENAGVIDIGAGLAAVFKMESHNHPSY
                     IEPYQGAATGVGGILRDVFTMGARPVANLNVLRFGSPDHPKTRHLVAGVVGGIGGYGN
                     CVGVPTVGGECSFDSRYDGNILVNAMCVGIARADRIFRARASGLGAPVVYVGSKTGRD
                     GIHGAAMASAAFGEDTEHKRPTVQVGDPFTEKLLLEACLELMATDAIVGIQDMGAAGL
                     TSSSVEMAAKGGAGIELDLDAVPVREAGMSPYEIMLSESQERMLMVLRPGAEARAREV
                     FDKWELDFAVIGRVTGSGRLVLRWHGEMVADMPVAPLAEGVRAERAWQAPPAPPPLPA
                     LPQIRAGDALRQLLSGPDIGSRLWIWDQYDHTVMADTVQGPGGDAAVVRVHGTTMGLA
                     LAADCTPRYCAADAALGAAQAVAETWRNLTAVGARPLAITDCLNFGNPERPEVMGEFV
                     HALDGIARACRSLDFPVVSGNVSFYNETEGRNIPPTPQVGGVGLIDDIARTARIALPG
                     PGLALVLVGETHGHLGQSLFARLLLGADLGPPPPVELEVERRNGDFVRALIAAGRVSC
                     CHDLSDGGLYVALAEMAIAGGVGADLAG"
BASE COUNT         1280 a         3171 c         3089 g         1258 t
ORIGIN      
        1 tgatcccccg ctacagccgg ccggagatgg ccgccatctg ggcgcccgag acgcgcttcc
       61 ggatctggct cgacatcgaa aggttcgcct gcgaagcgca ggagcagctc ggcgtgatcc
      121 cgcgcggcgt ggccaaggcg ctcgccgagc gcgggcgctt cgaggtcgcg cgcatcgccg
      181 agatcgaggc cgaggtcaag cacgacgtga tcgcgttcct gaccaacctc gccgagcatg
      241 tcgggccgga ggcgcgcttc gtccaccagg gcatgacctc ctccgacgtg ctcgacacct
      301 gcctcgcggt ccagctcacc cgcgctgccg acatcctgct cgacgatctc gacgcgctgc
      361 tggcggtgct cgagcggcgc gcgcgcgagc acaagctgac gccgtgcatc ggccgcagcc
      421 acggcatcca cgccgagccg accaccttcg gcctcaagct cgcccaggcc tatgccgagt
      481 tcgcccgcaa ccgcgaccgc ctggtcgccg cacgcgccga ggtcgcgacc tgcgcgatct
      541 cgggcgcggt cggcaccttc gccgacatcg atccgcgcgt ggaagcacac gtcgccgcga
      601 agctcgggct gagccccgag ccgatctcga cccaggtgat cccgcgcgac cgccacgcca
      661 tgttctttgc cacgctcggc gtgatcgccg cgtccgtcga gcgcctggcg accgaggtcc
      721 gccacctgca gcgcaccgag gtcggcgagg cggaggagta tttcgcgccc ggccagaaag
      781 gctcctcggc gatgccccac aagcgcaacc cggtgctgag cgagaacctg accgggcttg
      841 cccggctggt gcgcgcgacg gtcgccccgg cgctcgagga cgtagcgctt tggcacgagc
      901 gcgacatctc gcactcctcg gtcgagcgcg cgatcgcccc cgacgccacc gtcaccctcg
      961 acttcgcgct ggccaggctc gccggcgtga tcgacacgct ggtggtctac cccgaggcga
     1021 tgcggcgcaa cctcgacctg ctcggcggcc tgatccactc gcagcgcgtc ctgctggcgc
     1081 tgaccggcac cggcatgagc cgcgaggacg cctaccgcgc ggtgcaacgc aacgccatgc
     1141 gcgcgctgca gggcgacggc gacttcaagg cgctgctcgt cgcggacaag gaggtcgcgg
     1201 cgcgtctcga cgccaaggcg ctcgatgcgc tgttcgacct cgaccaccac ctggctcacg
     1261 tcgagaccat cttcgcgcgc gtgttcgggc caggggacgg cgccgggtga gggagccagc
     1321 ggcgcactgt tgcgcaccct ccgtcaaccc gtatatacat gtataggcga cggactcatt
     1381 gaggcgcgac gccgggtggg tcgaggcggc tgcgccgagc ctggggccgc caccaaggag
     1441 tgatttcgcc atgccgatca cgcgcgcgtt caagtccggc aacagccagg cggtgcgcat
     1501 cccggccgag ctcgcttacg acgacatgac catcgaactt caaatcacgc ggctcggcga
     1561 tgtcatcacc atcgcgccgg cacgccagag ccttaaagat gcggtcgcgg ccctgcgcag
     1621 actcccaagg ccgcccgccg tagagatcag ggagccgatc gaggtcccgg ttcgcgaata
     1681 gcgccggcga acgcacgcgc ccgtggctta cctcatcgac acgaacatcg ccatccacgc
     1741 ccgcgacggc acggactcgg ttctcgacaa gctggccgag catgacggca tggtcctgct
     1801 ctcggccctg agccttgccg agctgcaacg ggggatatat cgggacccgg cctggaccgc
     1861 gctgcggcaa gcgcggctgg agatcctgct tcgccacatc ccggtgctgc cgttcgatgc
     1921 cgccgccgcc attgcctacg gccggatcat cgccgaatgc ggatgggtca aggcgcggga
     1981 ctacgatcgc atgatcgccg cgcacgcgat cagcaccagc tcggtcctgg tcacggacaa
     2041 cgaggcggat ttccgggaca tatcgctttt gactattgag aattgggcga ttccgcgctg
     2101 acctgggcac ggccgaacgg ccgctgccaa tcgctcgcgg cgcgcgcgcc ctaccgctcc
     2161 cacgggccgc gcacggcggg cacgcgggtg cggcgtcgaa tgcgccatcc cggcggcggc
     2221 ggcggctgca gcggcgagcg cgggccgcgg ccgggcggcg ggcgcagcac caggatcagc
     2281 cccaccccgg ccaggaagcc gccgacatgc gcccaccagg cgatgccgcc ggccatcgcc
     2341 ggcgccagga tttcctgggc gccctggagc acctggagcg cgaaccagat cgcggcgaag
     2401 gcgagcgccg gcacctggaa cagcagcggc aggaacagca ccggcaccag cagcaccacg
     2461 cgcgccctgg ggaagcggac ggcgtaagcc ccgatcacgc cggcgatcgc gcccgaggcg
     2521 ccgagtgccg gcaccggcga ggccgcgttg aaggcgaaat ggctcgcgct ggcgatgatg
     2581 ccgctggcga agtacagcag cgtgaaccgg gccgcgccca gccggtcctc gaccgcgcag
     2641 ccgaagatcc acaaggtcca catgttgagg atcacgtgca gccagccgcc gtgcaggaag
     2701 gtgttggtca ggaacggcgc gtagtcggtc ggatcgagcc ccttcgcccg cgcccaggcg
     2761 ggctcggtgt agcgcagcgg gatcagcgcg tagtcgccga tcactgccgc caccccttgc
     2821 ggcggcagcg accagaccat caggaacacc agcgcgttga ccgcgatcag gccggccacg
     2881 accggagtcg ggcgccggca cggcacggag tcgcggatcg ggatcatcgc gcggcgtctc
     2941 ggcggtggtt ggcgggcgat aaccggttgt taaggctttc gccggactgt acgcgcgatg
     3001 agagcatttt gcctggcgtt catgctcgcc ctggcgctgg tgatgccgga cgctcgcacg
     3061 gcgacattgg tcgagaccag cattgccggc gagtccgttt gggtgatcga cgacccgctg
     3121 agcaagcgcg tgctggtcgc cagccgcgcc ggcagccggc tgatcgacct ggataccgag
     3181 accgtctatc tgatcgcgcc caacggatcc acgcagaagg tcgaagtctc ggcgctgccg
     3241 gcggtaaccg ccacctcgtc attcgagatc gagaaggtcg ggcccggtcc gcgcatcgcc
     3301 gactatccga ccacgcgctt ccggctcagc gccgagggcc ggacctgctc gctgatcgac
     3361 gccaatctcg gcctgggaga ggcgctcgag cgggcgatgc gcgccttcga cctgatggag
     3421 cgcctgcaaa gcgcgctcgg cggcgccgac cgcggcccgt gccagcgcat cccgctcggc
     3481 gagtatgcgc gcatcggctg gggcctcagg gtcgccgacg ccggcgcgcc ggtggttgag
     3541 gtggtgtcgg tggtgcgcaa ctacgagccg ctggccgagg agctcgcctt gcccacgtcc
     3601 gccgtcgaca tcaccatggt cgtgcgcgac ggcggcgcgc cggcgacgac gcgctgagcg
     3661 cgggctactc gctcatgatc tgccgcctgg cttcgggcag caggcgatcc atctcgaggc
     3721 ggatcttgtg ggcgtcgagc ggcacgcccc ggccctcgaa gtccttgagc accttctcca
     3781 ccacgtcgtc gtcgcccgga cgctccaggt ccgccagcac cacctcgcgg gcgtaggcct
     3841 gggccgcatc gccctcgagc ccgagcttgt cggcggccca cagcccgagc agcttgttgc
     3901 gccgggccat tgccttgaac cgcagctcct cgtcgtgctc gaacttcttc tccgcctcgc
     3961 gctcacggtt gtcgaatgtg gtcatcgccc tctcgtgcct gtgtcgttgg tcacacgcga
     4021 cgccacctcg tgggtgaccg gcccattgtg ccatccttcg ccgcggatac caattgagcc
     4081 gcgacgcgcg gccgctaaac tctgcctcct gcgcccgaga ccctatcgcc cggccacgac
     4141 ggaccatgtg cacccttgtc atcctgcgcc gatcgggcga ctcctggccg ctgatcctgg
     4201 ccgccaatcg cgacgaggtg ctcgcccgcc cgtgggcgcc gccggcgcgc cactggcccg
     4261 agcggcccca cgtggtggcc gggcgcgacc tcgaggccgg aggcacctgg ctcgggctgg
     4321 gcgacggcgg cgtggcggcg ggggtgctca accgcccggg cgcgctcggg ccggcgccgg
     4381 gcaaggcctc ccgcggcgag ctggtgctgc gggcgctcga ccacgattcg gccgcggccg
     4441 ctgtgcgcgc gctgcggcgc ctggacgcga cgcagtttcg gccgttcaac ctggtcgtcg
     4501 ccgacgccgg gggcgcgtgg tggctgcgct gggccggggc tgcgggcggc atcgaggcgg
     4561 cgccggtgcc ggaggggctg tcgatgatca ccgcccacga cctcaacgat accgcctcgg
     4621 cgcgcatccg ccgccagctg cccaggttcc gcgccgcgcc ggagcccgat ccggcgctgg
     4681 gcgactggtc cgcctggcag cgcctgctcg ccgactccga cggcggcgcc gacgggcctc
     4741 actcggccat gaccttgggc ccggccagcg gattcggcac cgtttcgagc tcgctgatcg
     4801 cgcttccggc ccgcgccggg acggccgaga gagcgcgctg gctgttcgcc cccgggcgtc
     4861 ccgatacgac cgagtttgag cctgtcgatc tttcgccaaa tcaaatgctt gaagggaaat
     4921 attcctctgg tagatgaatg tgtcgccgga ccccggcgag gcgggcgccg catcgggccg
     4981 gcattgtaca ggggggcgag ttggattatc taagggcgtg aaggcgcggc tcccacccgt
     5041 ccggcgtggg ggcgcttcta atttttgggg caacgcccgc aagaggggac caatggcccg
     5101 ccgcaggcaa atctacgagg gtaaggccaa agtcctgttc gaggggcccg agcccggcac
     5161 gctggtccag tacttcaagg acgacgcgac cgcgttcaac aaccagaagc gcggcaccat
     5221 caccggcaag ggcgtgctca acaaccgcat ctcggaatat ctgatgacgc ggctgtccga
     5281 gatcggcatc ccgacccact tcgtgcgccg gctgaacatg cgcgagcagc tgatcaagga
     5341 ggtcgagatc atcccgatcg aggtggtggt gcgcaacgtc gccgcgggct ccttgtcgac
     5401 ccgattcggc cttgccgagg gtacctcgct gccgcgctcg gtggtcgagt actactacaa
     5461 gtccgacgag ctcaacgacc cgatggtgtc cgaggagcac atcaccgcgt tcggctgggc
     5521 gaccccgtcg gagctcgacg agatgctgtc gatgtcgttg cgcgtcaacg acttcctgag
     5581 cggcctgttc ttcgccctcg gcatccgcct ggtcgacttc aaggtcgagt acgggcgcct
     5641 gttcgagggc gacgacatgc gcatcgtgtt ggccgacgag atcagcccgg acaactgccg
     5701 gctgtgggac atcaagacca acgagaagct ggacaaggac cgcttccggc gcgacctcgg
     5761 ccgggtcgag gaaggctatc aggaggtcgc ccggcgtctc ggcatcctgc ccgagagcgg
     5821 cccgcgcgac ctgcagggtc ccgacaccat gcagtgacct cgcgtctcgt gaaggccaag
     5881 gtccacatca ccctcaagga aggggtcctc gacccccagg gcaaggcgat cgagcacgcg
     5941 ctcaagatgc tcggattcgc tggcgtcgac caggtccgcc agggcaagta cctggagatc
     6001 gagctcgccg agaccgatcc ggccaaggcg ctgaaggcgg tcgaggggat gtgcgcccgc
     6061 ctgcttgcca acgcggtcat cgaggactac cagatcgagg tctcgtgacc ggcgcgcagc
     6121 ggcgcgggcc cgacaccgga ggctgacggc ggtggacgca gcggtggtcg tgttccccgg
     6181 ctccaactgc gaccgcgacg tggcggtggc gctgacccgg gcgtgcgggc gcgcgccggc
     6241 gatggtctgg caccgcgaga ccgagctgcc cgcggccgac ctgatcgtgc tgccgggcgg
     6301 cttctcctac ggcgactacc tgcgcccggg cgcgatggcg gcgcagtcgc cggtgatgcg
     6361 cgaggtggtc gcgcgcgccc aggccggcgt cgccgtgctc ggcatctgca acggctttca
     6421 ggtgctgacc gagtgcgggc tgttgccggg ggtgctgatg cgcaaccgcg gcctgcgctt
     6481 cctgtgccgc gaggcgccgc tcgcggtcga ggcggccgag cccccgttca ccgccggcta
     6541 ccgcgccggc gagcgcatcc gcctgccgat cgcccacaac gacggcaact tcttcgccga
     6601 cgaggatacc ctcgaccggc tcgagggcga aggcctggtc gcgctcaggt tcgtggccgg
     6661 gcccggggcg gcggagggcg cgaacgggcc ggcggcggcc aacggcgcct gccgcgacat
     6721 cgccggcatc gtcaacgcgc ggcgcaacgt gctcggcctg atgccgcatc ccgagcgcgc
     6781 ctgcgagccc gcgcacggca acaccgacgg cgcccgcctg ttcgccggcc tggcgtcgag
     6841 cctgtgagcg ggcgcgccca gaccgcggag gtgacgcccg agatcgccgc cgcgcacggc
     6901 ctggatgcgg ccgagtacgc cgacatccgg cacatcctcg gccgggtgcc gagcctgacc
     6961 gagctcggca tcttctcggc gatgtggagc gagcactgct cgtacaagtc gtcgcgcaag
     7021 tggctcgccc gcctgccgac cgcggcgccg tgggtgatcg aggggccggg cgagaacgcg
     7081 ggcgtgatcg acatcggcgc cggcctcgcc gcggtgttca agatggagag ccacaatcac
     7141 ccgtcctaca tcgagccgta tcagggcgcg gcgaccgggg tcggcggcat cctgcgcgac
     7201 gtgttcacca tgggcgcgcg gccggtggcc aacctcaacg tcctgcgctt cggcagcccc
     7261 gatcacccca agacccgcca cctggtcgcc ggcgtggtcg gcggcatcgg cggctacggc
     7321 aactgcgtcg gcgtgccgac cgtgggcggc gaatgcagct tcgattcccg ttacgacggc
     7381 aacatcctgg tcaacgccat gtgcgtcggc atcgcccgcg ccgaccgcat cttccgcgcc
     7441 cgggcgagcg gcctcggcgc gccggtggtc tatgtcggct ccaagaccgg gcgcgacggc
     7501 attcacggcg cggcgatggc ctcggccgcg ttcggcgagg acaccgagca caagcgcccg
     7561 accgtccagg tcggcgaccc gttcaccgag aagctgctgc tggaggcgtg ccttgagctg
     7621 atggcgaccg acgccatcgt cggcatccag gacatgggcg ccgccgggct gacctcgtcg
     7681 tcggtcgaga tggcggccaa gggcggcgcc gggatcgagc tcgacctcga cgcagtgccg
     7741 gtgcgcgagg ccggcatgag tccttacgag atcatgctgt cggagagcca ggagcgcatg
     7801 ctgatggtgc tcaggcccgg cgccgaagcg cgcgcgcgtg aggttttcga caagtgggag
     7861 ctcgacttcg cggtcatcgg gcgggtcacc ggcagcggcc ggctggtgct gcgctggcac
     7921 ggcgagatgg tcgccgacat gccggtcgcg ccgcttgccg aaggggtgcg tgccgagcgc
     7981 gcctggcagg cgccgccggc gccgccgccg ctgcccgccc tgccccagat ccgcgccggc
     8041 gacgcgctgc gccagctctt gtccggcccc gacatcggct cgcggctgtg gatctgggac
     8101 cagtacgacc acacggtgat ggccgatacg gtccagggcc cgggcggcga cgccgcggtg
     8161 gtgcgcgtgc acggcacaac catgggcctt gcgctggccg ccgactgcac gccccgctac
     8221 tgcgcggccg acgccgcgct cggcgccgcc caggcggtcg ccgagacctg gcgcaacctg
     8281 accgcggtcg gcgcccggcc gctggcgatc accgactgcc tcaacttcgg caatcccgag
     8341 cggcccgagg tgatgggcga gttcgtgcat gccctcgacg gcatcgcccg ggcctgccgc
     8401 tcgctcgact tcccggtcgt ctcgggcaac gtctcgttct acaacgagac cgagggacgc
     8461 aacatcccgc ccacccccca ggtcgggggc gtcggcctga tcgacgacat cgcgcgcacc
     8521 gcgcgcatcg cgctgcccgg cccgggactc gctttggtgc tggtcggcga gacccacggc
     8581 catctcggcc agtccttgtt cgcccgcctg ctgctcggcg ccgacctcgg cccgccgccg
     8641 ccggtcgagc tcgaggtcga gcggcgcaac ggcgacttcg tgcgcgcgct gatcgcggcc
     8701 gggcgggttt cctgctgcca cgacctgtcg gacggcgggc tctacgtggc gctcgccgag
     8761 atggcgatcg cgggcggagt cggcgccgac ctggccgg
//
LOCUS       DASWHU010000290         6155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2029160, whole genome shotgun sequence.
ACCESSION   DASWHU010000290 DASWHU010000000
VERSION     DASWHU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029160"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VGB88_10770"
     CDS             <1..422
                     /locus_tag="VGB88_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_040609789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HEX9647972.1"
                     /translation="VDYAQLVKIYGTAPEAARGRYSPAVCIGARKENIEGNPDPARVS
                     TSHVERQNLTMRMQMRRFTRLTNAFSKKIENHCHALALYFVWYNFCRQHKAHRLSPAM
                     AAGLADTLHDMEWIVGLVDARAPKPGPRGPYRKQNSN"
     gene            549..1352
                     /locus_tag="VGB88_10775"
     CDS             549..1352
                     /locus_tag="VGB88_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9647973.1"
                     /translation="MAAAGPADQRALSGRHGVVTGGGKGIGAAVAETLAGLGARVSVI
                     GRERARIDERVARIEHAYGVAAFAAACDVTDEAAVAHALADCAAALGPATILVNNAGA
                     ARSASFANTDRADWEAMISVNATATFLCTRAALPAMREAGWGRIVNIASTAGIKGYRY
                     VTAYSAAKHAVVGLTRSLALELAESGITVNAVCPGYTDTDLLAESVRRIAATTGMSEA
                     EARRKLAANNPMGRLMQPAEVANAVGWLCLPASAGITGQTIVVAGGEVS"
     gene            1352..1843
                     /locus_tag="VGB88_10780"
     CDS             1352..1843
                     /locus_tag="VGB88_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9647974.1"
                     /translation="MDHAPGTVTALARTESPAHDLRLWLRLFACAGMIEQRVRRGLRE
                     TFDTTLPRFDVLAQLDRAGEALTMGELSRRLMVSNGNVTGLVGRLVDEGLVERRASAS
                     DRRAQLVRLTAAGRRAFRAMTPVHARWIADALAGLEAGDKRRLLALLESLRVSLAGQK
                     ARP"
     gene            1840..2673
                     /locus_tag="VGB88_10785"
     CDS             1840..2673
                     /locus_tag="VGB88_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX9647975.1"
                     /translation="MTPSALRPISARELAPRHFRLAVADKVATVTLDRPERKNPLTFD
                     SYAELGETFRAFAHADDVRAVVVTGAGGNFSSGGDVHDIIGPLVGSSMPELLAFTRMT
                     GELMNAVIAAPQVVVAAIDGVCAGAGACLAMACDLRVGTADSKVGFLFTRVGLAGCDM
                     GACALLPRIIGHGRAAELLYSGRMMGGAEAHACGFYNRLCDAGRLAEEAQAIARELAD
                     GPAFAHAMTKRMLAQEWSMAIPQAIEAEAQAQAICMQTDDFRRAYEAFVARRKPVFEG
                     D"
     gene            2673..3872
                     /locus_tag="VGB88_10790"
     CDS             2673..3872
                     /locus_tag="VGB88_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9647976.1"
                     /translation="MADRSFLDWPFLEPAHRALAESLEVWAAEALATPRGHDDLDGAC
                     RTLVSDLAAGGWLELCVPASHGGRHQRLDVRSLCIARETLARHDALADFCFAMQGLGS
                     APISLFGDAPLKDRILPEVRAGTRIAAFALSEPDAGSDVAALATRARRDGEDYVLDGR
                     KTFISNGGIAGHYVVFARTGEAPGANGLSAFVVEADTPGLAVAERIQLIAPHPLATLA
                     FDGCRVPAGNMLGAPGDGFKVAMATLDIFRPTVAAAALGFARRALDAALGRAQTRSAF
                     GRPIAEHQLIQAKIADMACAVDAAALLVYRAAWAADRGGARIAREAAMAKLFATEAAQ
                     SVVDHAVQIFGGLGVVAGSVPERLYREVRALRIYEGTSEIQRLIVANETLKAAHHRQG
                     VSRHGTA"
     gene            3859..5529
                     /locus_tag="VGB88_10795"
     CDS             3859..5529
                     /locus_tag="VGB88_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9647977.1"
                     /translation="MAQRKRVRGWRPQGPSGHVDSFTRDRLPPPELQAPVDYSALPAL
                     AAYPDRINAADVLLGARVRAGDGGRTAVLFAGRAWSYAELDAVSARIAHVLVDDLGLV
                     PGNRVLLRAANNLMMAACWLAVLRAGGVCVATMPLLRARELTYIATKAEIRHALCDVA
                     LAAELEQTRAKVPALDRVSYFTATGDGGAGDADLDRRLAAKDTRFAAADTAADDVAII
                     AFTSGTTGQPKGCMHFHRDILAMCDCFPPHVFRSSRDDIVTGSPPLAFAFGLGGLLCF
                     PMRVGAAAALVDKPSPEALLDAIARYRCTALYTAPTAYRALTGQVGGHDISSLRKCVS
                     AGETLPAPTWHAWHEATGLKIIDGIGTTEMLHIFISSPQEEAVPGATGRVVPGYQACV
                     LADDGAPAAAGAIGRLAVRGPTGCRYLDDVERQKGYVEGGWNITGDVYRQDEDGYFWY
                     QARADDMIISAGYNIAGPEVEAALLEHADVLECGVVGEPDEKRGQIVAAHVVLRAGVP
                     RTADTAKALQDFVKQQIAPYKYPRRVVFADALPRTETGKLQRFKLSRG"
     gene            5694..>6155
                     /locus_tag="VGB88_10800"
     CDS             5694..>6155
                     /locus_tag="VGB88_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9647978.1"
                     /translation="MKVSVIGGGPGGLYFALLLKKARRAAEVTVYERNRPDDTFGFGV
                     VFSDATLDTFAAADAPSHREITESFAHWDDIDIHVKDELITSSGHGFSGLSRKKLMQI
                     LQRRAAALGVRLRFEREVPDPSRLAGSDLIVAADGINSAVREQYADHFQPRI"
BASE COUNT          880 a         2252 c         2181 g          842 t
ORIGIN      
        1 ctgtggacta tgcccagctt gtcaaaatct acgggacagc gcccgaggcc gcgcgtggtc
       61 gttacagccc ggcggtatgc atcggtgccc gcaaggagaa tatcgagggc aaccccgatc
      121 cggcccgcgt gagcacgtcg catgtcgagc ggcagaacct tacgatgcgg atgcaaatgc
      181 ggcggtttac gaggctgacc aatgccttct ccaagaaaat cgaaaaccac tgccacgcgc
      241 tggcgctgta tttcgtctgg tacaatttct gccggcagca caaggcgcac aggctaagcc
      301 cggcgatggc cgctggcttg gccgataccc tccatgacat ggagtggatc gtcgggctgg
      361 tcgacgcccg tgccccgaag cctgggccgc gcggtcccta ccggaagcaa aattcaaact
      421 gaggcactac cgatcggccg actggcttgt cgcctgccgt gccattattt tatacttcaa
      481 acaaacctac cggaaccagc gacatgccca cctccccggg caccgagacc ggacccgacg
      541 agaacaagat ggcggccgcc gggcccgccg accagcgggc gctgagcggg cgccacggcg
      601 tggtcaccgg cggcggcaag ggcatcggcg ctgcggtcgc cgagaccctg gccgggctcg
      661 gcgcgcgggt cagcgtgatc ggtcgcgagc gagcgcgcat cgacgagcgc gtggcgcgga
      721 tcgagcacgc gtacggcgtc gcggcgttcg ccgcggcgtg cgacgtgacc gacgaggctg
      781 cggtggcgca cgcgctcgcc gactgcgccg ccgccctcgg ccctgcgacg atcctggtca
      841 acaacgccgg cgcggcgcgg agcgcgtcgt tcgccaacac cgaccgcgcc gactgggagg
      901 cgatgatttc ggtcaacgcc accgccacct tcctgtgcac ccgggcggcg ctgccggcaa
      961 tgcgcgaggc cggctggggg cgaatcgtca acatcgcctc caccgccggc atcaagggct
     1021 accgctacgt caccgcctac tctgcggcca agcacgcggt ggtcgggctg acccgcagcc
     1081 tcgcgctcga gcttgcggag agcggcatca cggtcaacgc cgtgtgcccg ggctacaccg
     1141 acaccgacct gctggccgaa tcggtccggc ggatcgccgc cacgaccggc atgagcgagg
     1201 ccgaggcgcg gcgcaagctc gccgcgaaca atccgatggg ccggctgatg cagccggccg
     1261 aggtggcgaa cgcggtcggc tggctgtgcc tgccggcctc ggccgggatc accggccaga
     1321 cgatcgtggt cgcgggcggc gaggtgtcgt gatggaccac gccccgggca ccgtaaccgc
     1381 gctcgcccgc accgagtcgc cggcgcacga cctgcgcctg tggttgcggc tgttcgcctg
     1441 cgccggcatg atcgagcagc gcgtgcgccg gggcctgcgc gagaccttcg ataccaccct
     1501 gccccgcttc gacgtcctgg cgcagctcga tcgcgccggc gaggcgctga ccatgggcga
     1561 gctgtcgcgg cggctgatgg tgagcaacgg caacgtcacc ggcctggtcg gccggctggt
     1621 cgacgagggg ctggtcgagc ggcgggccag cgccagcgac cgcagggcgc agctggtgcg
     1681 gctgaccgcc gccgggcggc gcgcgttccg cgccatgact ccggtgcacg cgcgctggat
     1741 cgccgacgcg ctcgccggcc tggaggcggg cgacaagcgc cggctgctcg cgctgctcga
     1801 gtcgctccgc gtctcgctcg ccgggcaaaa ggcccgccca tgaccccgtc cgcgctacgc
     1861 ccgatctcgg cccgcgagct tgcgcccagg cacttccgcc tggccgtcgc cgacaaggtg
     1921 gcgaccgtca ccctcgaccg gcccgagcgc aagaacccgc tcaccttcga cagctacgcc
     1981 gagctcggcg agaccttccg cgcgttcgcc cacgccgacg acgtgcgcgc ggtcgtggtt
     2041 accggcgcgg gcggcaactt ctcgtccggc ggcgacgtcc acgacatcat cggcccgctg
     2101 gtcggcagct cgatgcccga gctgctcgcc ttcacccgca tgaccggcga gctgatgaac
     2161 gcagtgatcg cggcgccgca agtggtggtc gccgccatcg acggcgtctg cgccggcgcc
     2221 ggcgcgtgcc tggcgatggc ctgcgacctg cgagtcggca cggccgacag caaggtcggg
     2281 ttcctgttca cgcgcgtcgg cctcgccggc tgcgacatgg gcgcgtgcgc cctgctgccg
     2341 cggatcatcg gccacggccg cgccgccgag ctgctctaca gcgggcgcat gatgggcggc
     2401 gcggaagcgc acgcctgcgg cttctacaac cggctgtgcg acgcgggccg gctcgccgag
     2461 gaggcgcagg cgatcgcccg cgagcttgcc gacgggccgg ccttcgccca cgccatgacc
     2521 aagcgcatgc tcgcccagga atggtcgatg gcgattcccc aggcgatcga ggcggaggcg
     2581 caggcgcagg cgatctgcat gcagaccgac gacttccggc gcgcctacga ggcgttcgtc
     2641 gccaggcgca agcccgtctt cgagggcgac tgatggccga ccgctcattt ctcgactggc
     2701 cgttcctcga acccgcgcac cgtgcgctgg ccgagtcgct ggaggtgtgg gcggcggagg
     2761 cgctggcaac gccgcgcggc cacgatgatc tggacggcgc gtgccggacg ctggtttccg
     2821 atctcgccgc cggcggctgg ctcgagctgt gcgtgccggc gagccacggc ggccgccacc
     2881 agcggctcga cgtgcgcagc ctgtgtattg cgcgcgagac gctcgcccgc cacgatgcgc
     2941 tcgccgactt ttgcttcgcc atgcaaggat tgggcagcgc gccgatcagc ctgttcggtg
     3001 acgcgccgct caaggaccgg atcctccccg aggtgcgcgc gggcacgcgc atcgccgcgt
     3061 ttgcgctgag cgagcccgac gccggctccg acgtcgccgc gctggcgacc cgcgcgcgcc
     3121 gcgacggcga ggactatgtg ctcgacggcc gcaagacgtt catctccaac ggcggcatcg
     3181 ccgggcatta cgtcgtgttc gcgcgcaccg gcgaggcgcc gggcgcaaac ggcctgagcg
     3241 cgttcgtggt cgaggccgac acgcccggcc tggccgtcgc cgagcgcatc cagctgatcg
     3301 cgccgcatcc gctcgccacc ctcgccttcg acggctgccg ggtgccggcc ggaaacatgc
     3361 tcggcgcgcc cggagacggc ttcaaggtgg cgatggcgac cctcgacatc ttccgcccga
     3421 ccgttgccgc cgccgcgctc ggcttcgccc gccgcgcgct cgatgcggcg cttgggcggg
     3481 cgcagacgcg ctcagccttc ggccggccga tcgccgagca ccagctgatc caggccaaga
     3541 tcgccgacat ggcgtgcgcg gtcgacgccg ccgcgctgct cgtctaccgg gcggcgtggg
     3601 cggccgaccg gggcggggcg cgcatcgcgc gcgaggcggc gatggccaag ttgttcgcga
     3661 ccgaggcggc gcagagcgtg gtcgaccacg cggtgcagat cttcggcggc ctcggcgtgg
     3721 tcgccggcag cgtgcccgag cggctctatc gcgaggtgcg cgcactgagg atctacgaag
     3781 gcaccagcga gatccagcgg ctgatcgtgg ccaacgagac gctcaaggcc gcgcaccacc
     3841 gacagggagt gtcccgccat ggcacagcgt aagcgcgtgc gcggctggcg gccgcagggg
     3901 ccgtcgggcc acgtcgacag cttcacccgc gaccgcctgc cgccgcccga gctgcaggcg
     3961 ccggtcgact actcggcgct gccggcgctc gccgcctacc ccgaccggat caacgccgcc
     4021 gatgtgctgc tgggtgcgcg ggtgcgcgcg ggcgacggcg ggcgcaccgc ggtcctgttc
     4081 gccggccgcg cctggagcta tgccgagctc gacgcggtga gcgcgcgcat cgcgcacgtg
     4141 ctggtcgacg acctggggct ggttccgggc aaccgcgtgc tgctgcgcgc ggccaacaac
     4201 ctgatgatgg cggcatgctg gctcgcggtg ctgcgggcgg gcggcgtgtg cgtcgcgacc
     4261 atgccgctgt tgcgcgcgcg cgagctcacc tacatcgcga ccaaggccga gattcgccac
     4321 gcgctgtgcg acgtggcgct cgcggccgag ctcgagcaga cccgggccaa ggtgccggcg
     4381 ctcgaccggg tgtcgtactt caccgccacc ggcgacggcg gcgcgggcga cgccgacctc
     4441 gaccggcgcc tcgccgccaa ggacacgcgc ttcgccgccg ccgacaccgc cgccgacgac
     4501 gtcgcgatca tcgccttcac ctccggcacc accggccagc ccaagggctg catgcacttc
     4561 caccgcgata tcctggcgat gtgcgactgc ttcccgccgc acgtgttccg gtcgagccgc
     4621 gacgacatcg tcaccggctc gccgccgctc gcgtttgcct tcgggctcgg cgggctgctg
     4681 tgcttcccga tgcgcgtcgg cgcggcggcg gcgctggtcg acaagccctc gcccgaggcg
     4741 ctgctcgatg cgatcgcgcg ctatcgctgc accgcgctct ataccgcgcc caccgcctac
     4801 cgcgcgctca ccggccaggt cggcggccac gacatctcga gcctgaggaa atgcgtctcc
     4861 gccggcgaga cgctgccggc gcccacctgg cacgcctggc acgaggcgac cgggctcaag
     4921 atcatcgacg gcatcggcac caccgagatg ctgcacatct tcatctcgag cccgcaggag
     4981 gaggcggtgc ccggcgcgac cgggcgggtg gtgccgggct accaggcctg cgtgctcgca
     5041 gacgacggcg cgccggcggc ggcgggcgcg atcggcaggc tcgccgtgcg cgggcccacg
     5101 ggatgccgct acctcgacga tgtcgagcgc cagaagggct acgtggaggg cggctggaac
     5161 atcaccggcg acgtctaccg ccaggatgag gacggctact tctggtacca ggcccgcgcc
     5221 gacgacatga tcatctcggc cggctacaac atcgccgggc ccgaggtcga ggcggcgctg
     5281 ctcgagcacg ccgacgtgct cgaatgcggc gtcgtcggcg agccggacga gaagcgcggc
     5341 cagatcgtcg ccgcccatgt cgtgttgcgc gccggcgtgc cgcggaccgc ggacacggcc
     5401 aaggcgttgc aggacttcgt caagcaacag atcgcaccct acaagtaccc gcgcagggtg
     5461 gtgttcgccg atgcgctgcc gcgtaccgag accggcaagc tgcagcgctt caagctgagc
     5521 cggggctagg cgatgaccga ccttggtcgc ggccaccccc ctctcccagc tccgggtaac
     5581 gcgcgacggg cgccgtgcca accctgcgca accctctccc cccgcgtcgc ggggggagat
     5641 ggaagggtga ggggggcggc ggtgatcagc cgagacagag gcgtgcgcac aaaatgaagg
     5701 tcagcgtcat cggcggcggc ccgggcgggc tgtacttcgc gctgctgctg aagaaggcgc
     5761 ggcgcgccgc cgaggtcacc gtctacgagc gcaaccgccc cgacgacacc ttcggcttcg
     5821 gggtggtgtt ctcggacgcc accttggata ccttcgccgc cgcggatgcg ccgagccacc
     5881 gcgagatcac cgagagcttc gcccactggg acgacatcga catccacgta aaggacgagc
     5941 tcattacctc gtccggtcac ggcttcagcg gcttgtcgcg caagaagctt atgcagatct
     6001 tgcagcgccg cgctgccgcg ctcggcgtgc gcctgcgctt cgagcgggag gtgcccgacc
     6061 cctcgcggct cgccggatcg gacctgattg tcgccgccga cggcatcaac agcgcggtgc
     6121 gcgagcagta cgccgaccac ttccagccgc gcatc
//
LOCUS       DASWHU010000291         6959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1269527, whole genome shotgun sequence.
ACCESSION   DASWHU010000291 DASWHU010000000
VERSION     DASWHU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269527"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGB88_10805"
     CDS             complement(<1..303)
                     /locus_tag="VGB88_10805"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX9647979.1"
                     /translation="MAELARLAALIDAAERIVVFTGAGISTESGIPDFRSPGGIWSRY
                     RPIDFREFVASEPARREYWQRKFAVDPLIEGAEPNRGHRAVAELIAAGKARVVITQN"
     gene            complement(517..1521)
                     /gene="cobS"
                     /locus_tag="VGB88_10810"
     CDS             complement(517..1521)
                     /gene="cobS"
                     /locus_tag="VGB88_10810"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEX9647980.1"
                     /translation="MATPAAAPSAAHDADRLPDIEISVRQVFGIDSSMVVPAFSDAAE
                     HVPDFDDAYRFDRDTTLAILAGFAHNRRVMIQGYHGTGKSTHIEQVAARLNWPCIRIN
                     LDSHISRIDLVGKDAIVLRDGQQVTEFREGLLPWALQSPTALVFDEYDAGRPDVMFVI
                     QRVLEVEGKLTLLDQNRIIRPHPAFRLFSTTNTVGLGDTTGLYHGTQQINQGQMDRWN
                     IVATLNYLAPEKEIDIVLAKVPALDSAEGRHRAAAMVELANLTRAGFINGDISTVMSP
                     RTVITWAENAAIFDDLGFGFRVTFLNKCDETERAVVAEYYQRCMGEELPESATRATLH
                     "
     gene            complement(1577..2089)
                     /locus_tag="VGB88_10815"
     CDS             complement(1577..2089)
                     /locus_tag="VGB88_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX9647981.1"
                     /translation="MVLVRGCAWPGCEQAGDYRAPRAPSALRDYVWFCLDHVRQYNKE
                     WDFFAGMDASAIEEVRRRDAVWHRPTWPFGTRPDAGPGEMPRFHDPFGLLGDGGIEAE
                     PPRPNTPRDQALAELGLDHTVTQAEVKARYKELVKRHHPDANGGCKKSEERLKSINKA
                     YTFLRSCEIA"
     gene            2225..2503
                     /locus_tag="VGB88_10820"
     CDS             2225..2503
                     /locus_tag="VGB88_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX9647982.1"
                     /translation="MSIRDAIERKLKAALLPERLDIVDESRLHAGHAGARAGGDSHFR
                     VYIVARAFAGKTRIERQRLVNTLLADEFAAGLHALALTTLTPEEAGAR"
     gene            complement(2506..3084)
                     /locus_tag="VGB88_10825"
     CDS             complement(2506..3084)
                     /locus_tag="VGB88_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX9647983.1"
                     /translation="MPLPEPAPREHIHTRTIECTGFRRADGLWDIDGRLTDTKTYAFS
                     NRWRGELAPGEPVHDMRIRLTIDDDMVIRAVAATTEASPFEICPEVAPRLEVLAGERV
                     GEGWSRTVRRLVGGSRGCTHLTELLGRMATAAYQTIWPLRASEAERRGVPAQSEPPRR
                     LVDSCHAWRSDGPVMAELYPDYAPSKPRAAGD"
     gene            complement(3187..4473)
                     /locus_tag="VGB88_10830"
     CDS             complement(3187..4473)
                     /locus_tag="VGB88_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX9647984.1"
                     /translation="MSAPLLLTLAAILLLLLMSAFFSGSETAVTAASRSRMHHLAERG
                     DRRARLVARLIERREQLIGAVLFGNNAVNILASALATSLFITLVGEGAVVYATVVMTL
                     LIFVFAEVLPKTLAIRRADQVALAVAPTLRVVVVVLAPLTRAVQALVGAILRLAGTER
                     ADRRLLSTADELRGVFDLHAREGRVRKHYRDMLRSILDLSEVEVGEIMTHRRNMVTVD
                     AGQPVSKIVDEVLSSPHTRVPTWRDNPDNIVGVLHAKSLLRAVQQHAGEVDRMDIAEV
                     ANEPWFVPDTTSLAEQLAAFRGRHEHFALVVDEYGTLQGLVTLEDIIEEIVGEIRDEY
                     DMPTSGVRRQADGSIVVDGGVTIRDLNRALDCRLPDEEAATVAGLVVHEAEYIPEVGQ
                     KFAFHGFEWEVLGRDRSRITSLRLRAQGRERSAERA"
     gene            complement(4510..5646)
                     /gene="aroB"
                     /locus_tag="VGB88_10835"
     CDS             complement(4510..5646)
                     /gene="aroB"
                     /locus_tag="VGB88_10835"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9647985.1"
                     /translation="MSEGTTTTVRVKLAARSYDIVIGAGLLAEAGRRASEVMAGRRAL
                     IVTDRAVAERYLPALEQSLHRAGIASDRFVLAAGEHAKSFAGLESLLDDMLAARPERD
                     VTVIALGGGVIGDLAGFAASILLRGVAVVQVPTTLLAQVDSSVGGKTGINTRHGKNLV
                     GSFHQPRLVLADTAVLDSLPRRELCAGYAEVVKYALIDDAPLFDWLAANGAAVLGEGA
                     PDRVAAARAHAIAVCCRAKAAVVAADEHEGERRALLNLGHTFAHALEVESGYGGLLLH
                     GEAVAIGMVMAFDLSHRLGFCPREDLARVRAHLAAAGLPVAPPRIEGRAWQAGDLIAH
                     MKHDKKVRGGRAVLVLARGIGRAFLYRDATDEQILPVLEDALAA"
     gene            complement(5643..6206)
                     /locus_tag="VGB88_10840"
     CDS             complement(5643..6206)
                     /locus_tag="VGB88_10840"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9647986.1"
                     /translation="MTGDCRIPVQDRIIVLVGLMGAGKTTVGRRLADRLGLRFVDADN
                     EIEAAAGMSISNMFEAHGEAHFREGERRVIARLLEHRGLVLATGGGAFMDPDTRALIA
                     ERAISVWLKADLDVLYHRVARRSTRPLLRRDEPREVLERLIEERYPVYAEADVTVESG
                     DVPHDAMVDKIVVALKARIAAEGAAER"
     gene            6552..6692
                     /locus_tag="VGB88_10845"
     CDS             6552..6692
                     /locus_tag="VGB88_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647987.1"
                     /translation="MRFITRLFVLLVLVAVIGGAVFLATWDIPPPTATTTTDIPDDRF
                     PR"
     gene            6673..>6959
                     /locus_tag="VGB88_10850"
     CDS             6673..>6959
                     /locus_tag="VGB88_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9647988.1"
                     /translation="MIAFRAERVAVLAAALALASVSARAQDEGAPLRLVPLPGPAPEA
                     QPLAAPEEAGPAQPAPAAGEPEISIGRLEEVGVDAFGLLAPGDGGLGPELW"
BASE COUNT          976 a         2478 c         2464 g         1041 t
ORIGIN      
        1 gttctgggtg atcaccacgc gcgccttgcc cgcggcgatc agctccgcca ccgcgcgatg
       61 gccgcggttg ggctcggcgc cctcgatcag cgggtcgacc gcgaacttgc gctgccagta
      121 ctcgcgccgc gccggctccg aggcgacgaa ctcgcggaag tcgatcgggc ggtagcgcga
      181 ccagattccg cccgggctgc ggaagtccgg gatccccgac tcggtcgaga tgccggcgcc
      241 ggtgaacacc acgatccgct ccgcggcgtc gatcagggcg gcgagtcggg caagctccgc
      301 cattgccccg gggctcagag ccctaaaatc ttttgcctca cccttcgaga cgcgcccgtc
      361 gggcgctcct cagggtgagg taaaagattg attctcaaaa ccccctcgtc ctgaggagcg
      421 agcggcccgt cctccgcagc agcggcagcg ccgctgccgc tgcggagggt ggaagcgagc
      481 gtctcgaagg gcgacagccg attcattcgc agcctttcag tgcagcgtcg cccgggtcgc
      541 cgactcgggc agctcctcgc ccatgcagcg ctggtagtac tcggcgacga ccgcgcgctc
      601 ggtctcgtcg cacttgttga ggaaggtgac gcggaagccg aagccgaggt cgtcgaagat
      661 ggcggcgttc tcggcccagg tgatcaccgt cctcggcgac atcacggtcg agatgtcgcc
      721 gttgatgaag ccggcgcggg tcaggttggc aagctcgacc atcgccgccg cccgatgccg
      781 cccctccgcg ctgtcgagcg ccggcacctt ggccagcacg atgtcgatct ccttctccgg
      841 cgccagatag tttagcgtgg caacgatgtt ccagcgatcc atctgtccct ggttgatctg
      901 ctgggtgccg tgatacaggc ccgtggtgtc gccgagcccg accgtgttgg tggtcgagaa
      961 caggcggaac gccggatgcg ggcggatgat ccggttctgg tcgagcaggg tcagcttgcc
     1021 ctcgacctct agcacgcgct ggatcacgaa catcacgtcc ggccggccgg catcgtactc
     1081 gtcgaacacc agcgcggtcg gcgactgcag cgcccagggc agcaggccct cgcggaactc
     1141 ggtcacttgc tggccgtcgc gcagcacgat cgcgtccttg cccaccaggt cgatgcggct
     1201 gatgtggctg tcgaggttga tgcggatgca cggccagttg agccgcgccg ccacctgctc
     1261 gatatgggtc gacttgccgg tgccgtgata gccctggatc atcacccgcc ggttgtgggc
     1321 gaagccggcg aggatggcga gcgtggtgtc gcggtcgaag cgataggcgt cgtcgaagtc
     1381 ggggacgtgc tcggccgcgt ccgagaacgc cggcaccacc atgctggagt cgatgccgaa
     1441 cacctggcgc accgagatct cgatgtccgg cagtcggtcc gcatcgtggg ccgccgaggg
     1501 ggcggcggcg ggcgtggcca tgggctgtgt cgtcctgtct ggtgtcggcc tcgggcggaa
     1561 ggctcagcgc gcgccgtcag gcgatctcgc agctgcgcag aaaggtgtac gccttgttga
     1621 tcgatttcaa ccgttcctcc gacttcttgc aaccgccgtt ggcgtcggga tggtggcgtt
     1681 tcaccagctc cttgtagcgg gccttcacct ccgcctgggt caccgtgtgg tcgaggccga
     1741 gctcggccag cgcctgatcg cgcggcgtgt tgggccgcgg cggctcggcc tcgatgccgc
     1801 cgtcgccgag caggccgaag ggatcgtgaa agcgcggcat ctcgcccggc ccggcgtcgg
     1861 ggcgcgtgcc gaacggccag gtcggacgat gccagaccgc gtcgcgccgc cgcacctcct
     1921 cgatcgcgga ggcatccatg ccggcgaaga agtcccactc cttgttgtac tggcggacgt
     1981 ggtcgaggca gaaccagacg tagtccctga gcgcactcgg cgcacgcggc gcgcgatagt
     2041 cgccggcctg ttcgcatccc ggccatgcgc agccgcgcac caacaccacc gcctcgctgc
     2101 ctgcggtcca ccggcgccaa ctgccccaca cggttgaacg cgtcgtctcc atgcctcaaa
     2161 tatgacggcg atagggccgc agcacaagtc ggcccgcggg ttttcaccga ccgacgcgac
     2221 cgccatgtcg atacgggatg caatcgaacg caagctcaag gcagcgcttc tgcccgagcg
     2281 tctggacatc gtcgacgagt ctcgcctgca cgccggacat gccggtgcgc gcgccggcgg
     2341 cgacagtcat ttccgggtgt atatcgtcgc gcgcgccttc gccggcaaga cgcggatcga
     2401 gcgccagcgc ctggtcaaca ctctgctcgc cgacgagttc gccgccgggc tgcacgcgct
     2461 cgcgctgacc acgctgacgc cggaggaagc gggcgcccgc tgagctcagt cgcccgcggc
     2521 gcgcggcttg gacggcgcgt agtcgggata gagctcggcc atcaccgggc cgtcgctgcg
     2581 ccaggcgtgg cagctgtcga ccaggcgccg cggcggctcg ctctgcgccg gcaccccgcg
     2641 cctctcggcc tcgctcgcgc gcagcggcca gatggtctgg taggccgcgg tcgccatccg
     2701 ccccaagagc tcggtcagat gggtgcagcc gcggctgccg ccgaccagcc ggcgcacggt
     2761 gcgcgaccag ccttcgccga cgcgctcgcc ggccagcacc tcgagcctgg gcgcgacctc
     2821 gggacagatc tcgaacggcg aggcctcggt ggtcgcggcg accgcgcgga tcaccatgtc
     2881 gtcgtcgatg gtcagccgga tgcgcatgtc atgcaccggc tcgcccgggg ccagctcgcc
     2941 gcgccagcgg ttgctgaagg cgtaggtctt ggtgtcggtc aggcggccgt cgatgtccca
     3001 tagcccgtcg gcgcggcgaa agcccgtgca ttcgatggtg cgggtatgga tgtgctcgcg
     3061 cggcgcgggc tcgggaagcg gcatgtgggg gtctccgttg gcgcgggccg gcggcgcggc
     3121 cggcgcggtc accgtcctgc ggtcaaatag aggctcggcg cgcgccccgc aagggcgcga
     3181 tggcgttcag gcgcgctcgg cgctccgctc gcggccctgc gcgcgcaggc gcaacgaggt
     3241 gatgcggctg cggtcgcggc ccagcacctc ccactcgaag ccgtggaagg cgaacttctg
     3301 gccgacctcg gggatgtatt cggcctcgtg caccaccagc ccggccacgg tcgccgcctc
     3361 ctcgtcgggc agccggcagt cgagcgcgcg gttgaggtcg cggatggtca ccccgccgtc
     3421 gaccacgatg ctgccgtcgg cttggcggcg cacgccgctg gtcggcatgt cgtactcgtc
     3481 gcggatctcg ccgacgatct cctcgatgat gtcctcgagc gtgaccagtc cttgcagcgt
     3541 gccgtactcg tcgaccacca gcgcgaaatg ctcgtggcgg ccgcggaacg cggcgagctg
     3601 ctcggcgagg ctggtggtgt cgggcacgaa ccacggctcg ttggcgacct cggcgatgtc
     3661 catccggtcg acctcgccgg cgtgctgctg gaccgcgcgc aacaggcttt tggcgtgcag
     3721 cacgccgacg atgttgtcgg ggttgtcgcg ccacgtcggc acccgggtgt gggggctcga
     3781 cagcacctcg tccacgatct tggacaccgg ctggcccgcg tcgaccgtga ccatgttgcg
     3841 ccggtgggtc atgatctcgc cgacctcgac ctcggacagg tcgaggatgc tgcgcagcat
     3901 gtcgcggtaa tgcttgcgca cccggccctc gcgggcgtgc aggtcgaaca cgccgcgcag
     3961 ctcgtcggcg gtcgacaaca ggcgccggtc ggcgcgctcg gtgccggcca ggcgcaggat
     4021 cgcgccgacc agcgcctgca ccgcccgggt cagcggtgcc agcaccacca ccacgacgcg
     4081 caaggtcggc gctaccgcca gcgccacctg gtcggcgcgg cggatggcca aggtcttggg
     4141 cagcacctcg gcgaacacga atatcaacag cgtcatgacc acggtcgcgt agaccaccgc
     4201 gccctcgccg accagggtga tgaacaggct ggtggcgagc gcggaggcga ggatgttgac
     4261 cgcgttgttg ccgaacagca ccgccccgat cagctgctcg cggcgctcga tcaggcgcgc
     4321 gaccaggcgc gcgcgccggt caccgcgctc ggccaaatga tgcatgcgcg agcgcgacgc
     4381 ggcggtgacc gcggtctcgg agccggagaa gaacgccgac atcagcagca gcagcaggat
     4441 cgccgccagg gtcagcagca gcggcgcgct catgtcgcga ccgaatccgg cgcgccacgc
     4501 ccgctggcgt caggcggcga gagcatcttc aagcaccgga aggatctgct cgtcggtggc
     4561 atcgcggtag aggaaggcgc ggccgatgcc gcgggcgagc accagcaccg cgcgcccgcc
     4621 gcgcactttc ttgtcgtgtt tcatgtgcgc gatcaggtcg cccgcctgcc aggcgcggcc
     4681 ctcgatgcgc ggcggcgcca ccggcaagcc ggcggcggcg agatgggcgc gcacgcgcgc
     4741 aaggtcctcg cgcgggcaga agccgaggcg atgcgacagg tcgaacgcca tgaccatgcc
     4801 gatcgccacc gcctcgccgt gcagcaacag cccgccgtag ccgctttcga cctcgagcgc
     4861 gtgggcgaag gtgtggccca ggttgagcag cgcgcgccgc tcgccctcgt gctcgtccgc
     4921 cgccaccacg gcggccttgg cgcggcagca gacggcgatc gcatgggcgc gcgcggccgc
     4981 gacccggtcg ggcgcgccct cgccgagcac cgcggcgccg ttcgcggcca gccagtcgaa
     5041 cagcggcgca tcgtcgatca gcgcgtactt gaccacctcc gcatagccgg cgcaaagctc
     5101 gcgtcggggc aggctgtcga gcaccgccgt gtcggccagc accaggcggg gctggtggaa
     5161 gctgccgacc aggttcttgc cgtgccgggt gttgatgccg gtcttgccgc cgaccgagct
     5221 gtcgacctgg gcgagcaggg tggtcggcac ctgcaccacc gccaccccgc gcagcaggat
     5281 gctggcggcg aagccggcaa ggtcgccgat cacgccgccg cccaaggcga tcacggtgac
     5341 gtcgcgctcg gggcgcgccg ccagcatgtc gtcgagcagc gattcgagcc cggcgaagct
     5401 cttggcgtgc tcgcccgcag ccagcacgaa gcggtcgctg gcgatgccgg cgcgatgcag
     5461 gctctgctcg agtgccggca ggtacctctc ggccaccgcc cggtcggtca cgatcagcgc
     5521 ccgccgtccc gccatcacct cgctcgcccg gcgcccggcc tcggccagca gcccggcgcc
     5581 gatgacgatg tcgtagctgc gtgccgccag cttcacccgg accgtggtcg tggtgccctc
     5641 gctcatcgtt cagccgcgcc ctccgccgcg atccgcgcct tgagcgcgac cacgatcttg
     5701 tcgaccatcg cgtcgtgggg cacgtcgccg ctttccaccg tcacgtccgc ctcggcatag
     5761 accggatagc gctcctcgat cagccgttcc agcacctcgc gcggttcgtc gcggcgcagc
     5821 agcgggcgcg tgctgcggcg ggcgacgcgg tgatagagca cgtcgagatc ggccttgagc
     5881 cagaccgaga tcgcgcgctc ggcgatcagc gcccgcgtgt cggggtccat gaacgcgccg
     5941 ccgccggtcg ccagcacgag cccgcggtgc tcgagcaggc gggcgatcac ccgccgctcg
     6001 ccctcgcgga aatgggcctc gccatgggcc tcgaacatat tgctgatcga catgccggcg
     6061 gcggcctcga tctcgttgtc ggcgtcgacg aagcgcaggc ccaggcggtc ggcgaggcgc
     6121 cgcccgacgg tggtcttgcc ggcgcccatc aggccgacca gcacgatgat acggtcctgc
     6181 accggtatcc gacagtcgcc ggtcaccgta tgccggccgt cgcccagccg gcgcgcgcgc
     6241 cgcacatgcg ccttgcgccg gtcatgcgcg ccgcatgccg caatcgggcc ttgacgggat
     6301 agcggcggcg gcacaacgcg gtggccatct attgtgcaag cgagtccgag tccgtatacc
     6361 aatggtcgga tacggcgagc cgcgtcatcg gcgcggcgtc gcccggtccg cgcctgacgc
     6421 gcatcggatc gagccgcggg cgaacgggtc ggtatatccg ctcattggtg gttgacttgg
     6481 ccactccgga acgggcgtga ctatagcaca gccgaatcac actgtcccaa aagcgtcggc
     6541 cgggtaagcg gatgcgcttc atcacccggc tgttcgtgct gttggtcctg gtcgccgtca
     6601 tcggcggcgc cgtgtttctg gccacctggg acataccgcc gccgaccgcg accacgacca
     6661 cggacattcc cgatgatcgc tttccgcgct gagcgcgtgg cggtgctggc ggcggcactg
     6721 gcgctggcga gcgtgtccgc ccgcgcccag gacgagggcg cgccgctgcg cctggtgccg
     6781 ctgccgggcc cggcgcccga ggcccagccg ctcgccgcgc ccgaggaggc ggggcccgcc
     6841 cagccggcac cggcggcggg cgagcccgag atctcgatcg gccgcctgga ggaggtcggc
     6901 gtcgacgcct tcggcctgct cgcgccgggc gacggtgggc tcgggcccga gctgtggca
//
LOCUS       DASWHU010000292        17791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3138403, whole genome shotgun sequence.
ACCESSION   DASWHU010000292 DASWHU010000000
VERSION     DASWHU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17791
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138403"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VGB88_10855"
     CDS             complement(<1..121)
                     /locus_tag="VGB88_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012557166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9647989.1"
                     /translation="MTVVSMTGFARRQGQDARHGWTWELKSVNHRAFDLRCRLP"
     gene            complement(248..1234)
                     /gene="mltG"
                     /locus_tag="VGB88_10860"
     CDS             complement(248..1234)
                     /gene="mltG"
                     /locus_tag="VGB88_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9647990.1"
                     /translation="MRLIGGLVSLVLLLALCAGVGGLWLVRAMDRPGPLAEESLVEVA
                     GGGGVGEIAALLEQAGVIKNAVLFEVGARLLAGGDTLKAGEYLIPAGVSARAVLDMLI
                     GGRTFARKLTVPEGLTARQVVALIEQAPGLAGEVEAVPPDGSLLPETYHYSRGDMRAE
                     VLARMRQALDETLAELWPRRADGLPYDSPEQALVLASIVEKETALEAERRHVAGVFVN
                     RLRRGMRLQSDPTVIYGLTGGAAPLGRPLTGADLDADNAFNTYVIGGLPPQPIANPGR
                     ASIAAVLDPADTEDLYFVADGAGGHVFARTLEEHNRNVAKWRRLVERASESR"
     gene            complement(1281..2537)
                     /gene="fabF"
                     /locus_tag="VGB88_10865"
     CDS             complement(1281..2537)
                     /gene="fabF"
                     /locus_tag="VGB88_10865"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9647991.1"
                     /translation="MRRVVVTGMGMVSPLACGVDASWRRLLEAESGISAIKAFDASDL
                     PARIAAQVPREGEDAFSPDDYIEPKEQRKMDPFIQHAIAASTQAVEDAGWTPADEHDR
                     ERTGVLIGSGIGGLPSIEAATMVLHEKGPRRISPFFIPSCLINLAAGQVSIRYGFKGP
                     NHSVVTACSTGAHAIGDAARLIMWGDADVMVAGGAEAAISRLGIAGFAAARALSTNFN
                     DQPTRASRPWDKDRDGFVMGEGAGIVVLEDYEHAKQRGARIYAEFVGYGLSGDAYHIT
                     APDPNGEGGYRAMRAALERAQMSPDDIDYINAHGTSTPLGDEIELGAVKRLFGHSAYK
                     LSMSSTKSSIGHLLGAAGAVEAIFSILAVVHDVVPPTLNLESPSEGCDIDLVPNEAKE
                     RPVRAALSNSFGFGGTNASLIFRKPS"
     gene            complement(2618..2860)
                     /locus_tag="VGB88_10870"
     CDS             complement(2618..2860)
                     /locus_tag="VGB88_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9647992.1"
                     /translation="MSDGNDVAGRVKKIVVEHLGVDESKVTESASLMDDLGADSLDAV
                     ELVMAFEEEFGCEIPDDAADKIVTVQDAIDYISEHV"
     gene            complement(3046..3783)
                     /gene="fabG"
                     /locus_tag="VGB88_10875"
     CDS             complement(3046..3783)
                     /gene="fabG"
                     /locus_tag="VGB88_10875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX9647993.1"
                     /translation="MFDLTAKGALVTGASGGIGGAIARALHRAGAAVALSGTRIDALQ
                     ALAGELGARAHVLACDLADAAAVAALAGDAEAALGRLDILVNNAGLARDGLMVRMKDE
                     DWQAVIEVNLSAPFRLSRAALRPMMRRRYGRIINVTSIVAVTGNPGQANYAAAKAGMI
                     AMSKSLAAEVAGRGITVNCIAPGMIDTAMTRALDADQRARLASAIPCGRLGSGEDVAA
                     GAVYLASDEAAYVTGQTLHINGGMAMV"
     gene            complement(3830..4771)
                     /gene="fabD"
                     /locus_tag="VGB88_10880"
     CDS             complement(3830..4771)
                     /gene="fabD"
                     /locus_tag="VGB88_10880"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9647994.1"
                     /translation="MARAFVFPGQGSQVVGMGGELCDAFQAARLVYEEVDEALGQRLS
                     RVILDGPESELSLTENAQPALMATSLAVVRVLEDEAGLRLAEAGCFVAGHSLGEYSAL
                     AAAGALSLADTARLLRQRGRAMQQAVPVGEGAMAAVLGLELDKVRCLAGAAANGAVCA
                     LASDNAPGQVVVSGAASAIDRLIELAPARGARRAVRLQVSAPFHCALLAPAAAEMAAA
                     LDSIAIAAPAPALVANVTAEAVVEPARIRALLVEQMTAMVRWRESVLYMKDHDVDTLV
                     ELGAGKVLANLARRIDPELAGVAVLTPHDVEALARSL"
     gene            5050..5538
                     /gene="rpsF"
                     /locus_tag="VGB88_10885"
     CDS             5050..5538
                     /gene="rpsF"
                     /locus_tag="VGB88_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9647995.1"
                     /translation="MPLYESVFIARQDVPAQDVEALAERYAGILAEHGGRVAKREYWG
                     LKNLAFRIKKNRKGHYTMMQLEAPADAVHEMERNMRISEDVLRYLTVRIDKVDDEPSV
                     MMQARSARDDRARRDDRRGGRGGEFRPRAEATGERAEAVRKDAGEAQASADAGPVKEE
                     SR"
     gene            5539..5802
                     /gene="rpsR"
                     /locus_tag="VGB88_10890"
     CDS             5539..5802
                     /gene="rpsR"
                     /locus_tag="VGB88_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9647996.1"
                     /translation="MADAKSSARPVPLPRRPFFRRRKSCPFSGANAPKIDYKDVKTLQ
                     RFTSERGKIVPSRITAVSAPKQRRLAQAIKRARFLALMPYLLK"
     gene            5844..6494
                     /gene="rplI"
                     /locus_tag="VGB88_10895"
     CDS             5844..6494
                     /gene="rplI"
                     /locus_tag="VGB88_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9647997.1"
                     /translation="MEVILLERVENLGQMGDVVRVRDGFARNYLLPRQKALRATKGNR
                     ERFERQRTRLEADNLTHRQEAEQVAKRMAGVSVVMVRQAGDTGQLYGSVNARDIAAAV
                     TEAGYTVDRRQVALNQVIKSLGLHPARLVLHPEVSVEVKVNVARSQEEAKLQAAGVDV
                     TALDREEAEREAAAAAAEAEAAKLFEEQEDAARAVVSARDEDEPAAGASEDQADKD"
     gene            6635..7864
                     /locus_tag="VGB88_10900"
     CDS             6635..7864
                     /locus_tag="VGB88_10900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX9647998.1"
                     /translation="MLLEGLLRGLIRHGRLTVIRHDGRQRTFGSAPGAEVVIRLHDRA
                     TAWRLALSPTLATAEAYMDGRLTLDKGELYDLLALAAANIERSGKPLVMRVRDALSRL
                     TRWFRQYNPAGRARRNAAHHYDLSDELYELFLDSNRQYSCAYFTDPGQDLEGAQRAKM
                     RHIAAKLLLAPGQTVLDIGSGWGGLGLELAREAGVEVRGVTLSEEQHKVATARAAKAG
                     LGDRVKFALADYRHEAGHYDRIVSVGMFEHVGVGHYGAFFAKLAALLKPDGVALLHTI
                     GRSDGPGSTDPFARKYIFPGGYTPALSEVVRPIERARLVITDIEVLRLHYAETLRAWR
                     TRFKANWDKAKALYDERFCRMWEFYLAGSEVSFRHLGMVVFQIQLAHRQDAVPLTRDY
                     VTDHDRSPDAKDSVAAE"
     gene            complement(8196..8822)
                     /locus_tag="VGB88_10905"
     CDS             complement(8196..8822)
                     /locus_tag="VGB88_10905"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX9647999.1"
                     /translation="MAGTDHDPPARLIVGISGASGAIYGVRLLELLRACAVESHLVMS
                     HSAEVTLAHETDRKLAEVRALADAWHPVDDLAASLSSGSYPTLGMIVAPCSVRCMSEI
                     ATGLCANLLTRAADVVLKERRPLVLMVRETPLHAIHLANMTRLAELGVVIAPPVPAFY
                     ARPQSLEEMIDHTLGRVLDLFGLDAGTVTRWGDSATGKAGRRPRRSPA"
     gene            complement(8828..10288)
                     /gene="rfaE2"
                     /locus_tag="VGB88_10910"
     CDS             complement(8828..10288)
                     /gene="rfaE2"
                     /locus_tag="VGB88_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX9648000.1"
                     /translation="MTELANLAGDVERLGAVRVLCVGDLMLDRYVYGTVERMSPEGPA
                     PVMRIVAEATMLGGAGNVVRNLVSLGARCTFVSVIGGDPVGRELEALLTSSPAVEPHL
                     LVDSEHGSIVKSRYVADGQQLLRADRDPLAPLPSQVTAEVSRLAAEAVPACDAVVLSD
                     YGKGVLDGPVVQAVVECARRCARPVVVDPKGLDFNRYRGADVLTPNWLELGAEAGAKI
                     DDDTSAVVAARRVIQRCGIQRVLVTRGRNGMSLVDFSHTEHIPAHTQEVLDMSGAGDT
                     VAATFTAAIAAGIDMLDAARLANFAAGIVVGKVGTAAVKADELRQALRHGSVRAADTK
                     VLAMEPAAKRIAMWRRQGQKIGFTNGCFDLLHPGHISLLRQARAACDRLVVGLNSDAS
                     VARIKGPGRPIQPEAARAMLLAALEHIDLVVVFEDDTPMRLLRLFRPDVLVKGADYLL
                     SEVVGAEFVKSYGGDVLLAEITPGYSTTSTIERIAG"
     gene            complement(10322..10912)
                     /locus_tag="VGB88_10915"
     CDS             complement(10322..10912)
                     /locus_tag="VGB88_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX9648001.1"
                     /translation="MAQPLMPKATAVWLVENTTLTFDQIADFCGLHPLEVQGIADDEV
                     AVGIVGLDPVGNGQVSRADIKRCEADPKARLKLLSAEVAAAPKRKGPRYTPVSRRGDR
                     PDGIAWLLKYTPELTDAQICKLVGTTKPTIEAVRSRSHWNISNIQPRDPVLLGICSQG
                     ELEEQLARSRRRLAREQAADAAQDGAAAGPADSQGG"
     gene            complement(11050..12027)
                     /locus_tag="VGB88_10920"
     CDS             complement(11050..12027)
                     /locus_tag="VGB88_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX9648002.1"
                     /translation="MTAVEISAPGGPEVLRAVTRPVPVPGHGEVLIRVAAAGVNRPDV
                     MQRAGSYPPPPGASDLPGLEVAGTVVRAGAGVAGPAPGEAVCALVTGGGYAEFCVAPA
                     DQTLPIPAGLDAVQAAALPETYFTVWTNLFERGRLDGGESVLVHGGSSGIGTTAVQLA
                     DAFGARVFATAGSPEKCRACESLGAERAIDYRSEDFLAVVKDATAGRGVDVILDMVGG
                     DYLKRNLAALADDGRLVIIAFLGGSAAEVDLMPILRRRLTVTGSALRPRTVERKSQIA
                     EALRAHVWPLLESGRVRPVIHRTFPLTSAAEAHALMEAGRHVGKIVLAV"
     gene            complement(12048..12893)
                     /locus_tag="VGB88_10925"
     CDS             complement(12048..12893)
                     /locus_tag="VGB88_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9648003.1"
                     /translation="MPETPDFFVRFWGVRGSIACPGPGTMRYGGNTSCLEVNCDGRLL
                     VLDAGTGIHALGTDIVRNRPHDGPFTADLFFTHTHFDHICGFPFFAPAFAAENRLSIW
                     AGHLLPERTVREVLIYLMMDPLFPIPIDTMHAELDFHDFVAGEALQPHDGVVVRTAPL
                     NHPNRATGYRIEYGGRSICYVTDTEHVPGKPDRNILGLIEGADLFIYDSTYTDDEFAD
                     HLEWGHSTWQEGVRLADEAKTKTLVVFHHNPDHDDDFMDRIAGEAEKARAGTLVAREG
                     MVLTP"
     gene            13128..13319
                     /locus_tag="VGB88_10930"
     CDS             13128..13319
                     /locus_tag="VGB88_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX9648004.1"
                     /translation="MFDDDDRPARPARTIPRNLDKMSIAALEDYIAELEGEIARVRQD
                     ISAKQRLQSEAEKVFKSGR"
     gene            13628..14923
                     /locus_tag="VGB88_10935"
     CDS             13628..14923
                     /locus_tag="VGB88_10935"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX9648005.1"
                     /translation="MAEAKFLNAETLMLHAGQHPDPEFGARAVPIFQTTSYVFPDTDT
                     AASMFNLERAGHLYSRISNPTVAVLEERLSALERGVGGVCTASGQASLHLAVATLMNA
                     GGHIVASKSIYGGSYNMMGHTLPRFGITTTFVDARDPAAFRDAIRPETRLLFGETLGN
                     PGLEVMDIAAVAEIAHAARLPLLIDNTFATPHLCRPFEFGCDLVMHSATKFLGGHGIA
                     IGGALIDSGRFDWEQSGKFPTMTEPYEGYHGLDFAEEFGPAAMIMRARAEGLRDFGGC
                     MSPQNAFYILLGVETLAVRMERHVANARKVVEYLCGHDAVEWVTWPELPEHPDYELAK
                     RMLPKGCGAIFSFGIKGGRAAGRSFIERLKLFSHLANVGDAKSLVIHPASTTHQQMSA
                     AELKAAGVGEELVRLSVGLEDPGDLIDDLGQALRASQRA"
     gene            14927..15724
                     /locus_tag="VGB88_10940"
     CDS             14927..15724
                     /locus_tag="VGB88_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9648006.1"
                     /translation="MELEVAGAPVHAATGGVALDPARPLIVFVHGAGMDHTVWALQAR
                     YFAHNGYGVLAVDLPGHGRSAGEALDSIEALGRWLWRLVDAAGPEHARLVGHSMGALA
                     ALEAAAAAPARARGLVLIGANAAMPVNDELLSAAREEPARAAALIVSWAYGERSHMGG
                     MRAPGLWMLGGGLSLLARARAGVLHADFAACNSYRGGLAAAARVSCPTLVLAGRGDRM
                     TPARGAAKLAEAIPGARLAVIEDAGHMPMIEQPDRTLDALRAQFAAP"
     gene            15721..16173
                     /locus_tag="VGB88_10945"
     CDS             15721..16173
                     /locus_tag="VGB88_10945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9648007.1"
                     /translation="MTETEARRRTDYAYLRAVPTRWNDNDLYGHLNNVAYYEIFDTVI
                     NRFLIEEGGLDIARGPVIGIVPETRCRYLKPLRYPDVIDVGLAVARLGRTSVVYDIAL
                     FRQGEDDAAALGHFVHVFVARAAQDRTRPIPRRVRSALARIARAPEED"
     gene            complement(16179..16409)
                     /gene="rpmE"
                     /locus_tag="VGB88_10950"
     CDS             complement(16179..16409)
                     /gene="rpmE"
                     /locus_tag="VGB88_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9648008.1"
                     /translation="MKTDIHPDYHEITVVMTDGSEFKTRTTWGKEGDVMKLDVDSLSH
                     PAWTGGAQRLVDTEGQVARFNKRFAGFGLGKK"
     gene            16647..>17791
                     /locus_tag="VGB88_10955"
     CDS             16647..>17791
                     /locus_tag="VGB88_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9648009.1"
                     /translation="MTLADARASDRPRSRELRHLAKLAGYLLPYRWVIAGAIGAVAVA
                     AATVLSLGIGLRLLVDEGFGGGDPALLDRALVALVAAVVVLASATYGRFYLVSWIGER
                     VIADIRRDVYNHVIALSPAFFETLRTGEVMSRLTADTTLLQVVIGSSASVALRNLLLF
                     AGGSVMLAVTSAKLTLITVVLVPLVVLPIVVFGRRVRRLSRTAQDRVADVSAQVEESV
                     AAIRTVQAFTHEAHDRARFAGHVESAFGAAVARVRARALLTALVMCSTFGGVALVLWI
                     GGRDVLAGGLSAGELSQFVFFAVVVAGALGAISEVYGDLQRAAGATERLLELLDARSE
                     VAAPAHPVPLPKPAQGAIAFQAVTFFYPSRPDAASLTELTLDIAPGER"
BASE COUNT         2550 a         6376 c         6304 g         2561 t
ORIGIN      
        1 gcggcaggcg gcagcgcagg tcgaaggcgc ggtggttgac gcttttcagc tcccaggtcc
       61 agccgtgccg cgcgtcttgt ccctggcggc gggcgaaccc ggtcatgctg acgacggtca
      121 cggacacctc ccggacccgc aggccgcgga gggtttcccc ggcggcggcg gccggacctg
      181 tatacggtgc gcggattccc gacacaagcc gaccgggagc gcgcccgccg aggccgccgg
      241 cgccggctca ccggctctcg ctcgcgcgct ccaccaggcg ccgccacttg gcgacgttgc
      301 ggttgtgctc ctcgagggtg cgcgcgaaca cgtgcccgcc cgcgccatcg gcgacgaagt
      361 agaggtcctc ggtgtcggcc gggtcgagca ccgccgcgat cgaggcgcgg cccgggttgg
      421 cgatcggctg gggcggcagg ccgccgatga cgtaggtgtt gaaggcattg tcggcgtcga
      481 ggtcggcgcc ggtcagcggc cggccgagcg gcgccgcgcc gccggtcagg ccgtagataa
      541 cggtcgggtc ggactgcagc cgcatgcccc ggcgcaaccg gttgacgaac acgccggcga
      601 cgtggcgccg ctcggcctcg agcgcggtct ccttctcgac gatcgaggcc agcaccagcg
      661 cctgctcggg gctgtcgtac ggcagcccgt ccgcgcgccg gggccacagc tcggcgagcg
      721 tctcgtcgag cgcctgccgc atgcgcgcca gcacctcggc gcgcatgtcg ccgcggctgt
      781 aatgataggt ctcgggcagc aggctgccgt ctggcgggac cgcttcgacc tcgcccgcga
      841 gcccgggcgc ctgctcgatg agcgccacca cctggcgcgc ggtgagccct tcgggcacgg
      901 tcagcttgcg tgcgaaggtg cggccgccga tcagcatgtc gagcaccgcg cgcgcgctca
      961 ccccggccgg gatcaggtac tcgccggcct tgagcgtgtc gccgccggcg agcaggcggg
     1021 cgccgacctc gaacagcacc gcgttcttga tcaccccggc ctgctcgagc agcgccgcga
     1081 tctcgccgac cccgccgccg ccggcaacct cgaccagcga ctcctcggca agcggccccg
     1141 ggcggtccat ggcgcggacc agccacagcc cgccgacgcc cgcgcacagc gccagcagca
     1201 ggacgagcga aaccagcccg ccgatcagcc gcatcgtgcc tgccgcggcg gcgcgccggc
     1261 caccgcccgc cggccggggc tcaggacggc ttgcggaaga tcaggctggc gttggtgccg
     1321 ccgaagccga aggagttgga cagcgccgcc ctgaccgggc gctccttggc ctcgttcggc
     1381 accagatcga tgtcgcatcc ctcagacggg ctctcaaggt tgagcgtcgg cggcaccacg
     1441 tcgtggacca ccgccaggat cgagaagatc gcctcgaccg cgccagcggc gccgagcaga
     1501 tgcccgatcg aggacttggt cgaggacatc gacagcttgt aggccgagtg cccgaacagg
     1561 cgcttgaccg caccgagctc gatctcgtcg ccgagcgggg tcgaggtgcc gtgggcgttg
     1621 atatagtcga tgtcgtccgg cgacatctgc gcgcgctcga gcgccgcgcg cattgcgcga
     1681 tagccgcctt cgccattcgg atcgggcgcg gttatgtgat aggcgtcgcc cgacaggccg
     1741 taaccgacga actccgcgta gatcctggcg ccgcgttgct tggcatgctc atagtcttca
     1801 agcacaacga tgccggcgcc ctcgcccatg acgaagccgt cacggtcctt gtcccacggc
     1861 cgcgacgccc gcgtcggctg gtcgttgaag ttggtcgaca gcgcgcgcgc cgcggcgaag
     1921 ccggcgatgc cgagcctgga tatcgccgct tccgcgccgc ccgcgaccat cacgtcggca
     1981 tcgccccaca tgatcagccg cgcggcgtcc ccgatcgcgt gcgccccggt cgagcaggcg
     2041 gtgaccaccg aatggttggg gcccttgaag ccgtaacgga tcgaaacctg cccggcggca
     2101 aggttgatca ggcagctcgg gatgaagaac gggctgatcc gcctcggccc cttctcgtgc
     2161 agcaccatgg tcgcggcctc gatgctgggc aggccgccta tgcccgagcc gatcagcacg
     2221 ccggtgcgct cgcggtcgtg ctcgtccgcg ggcgtccagc cggcgtcctc caccgcctgc
     2281 gtcgacgccg cgatcgcatg ctggatgaag gggtccatct tgcgctgttc cttgggctcg
     2341 atgtagtcgt cggggctgaa cgcgtcctcg ccctcgcgcg gcacctgggc ggcgatccgc
     2401 gccggcaggt ccgaggcgtc gaacgccttg atcgccgaaa tcccagactc ggcctcgagc
     2461 agccgccgcc agctcgcgtc gaccccgcat gccaggggcg acaccatccc catcccggtg
     2521 acgacgacgc gcctcatggt cttcgctccc cgcctgccgc gttgcgccgg ggcctcgcgg
     2581 ccacgcgccg ggcccggccc ggccgatcgt gccgcgacta gacgtgctcg ctgatgtagt
     2641 cgatggcgtc ctgaacggtg acgatcttgt ccgccgcgtc gtccggaatc tcgcagccga
     2701 actcctcctc gaacgccatc accagctcga cggcgtcaag gctgtccgcg ccgagatcgt
     2761 ccatcaggct ggcgctttcg gtcaccttcg attcgtccac cccgaggtgc tcgacgacga
     2821 tcttcttgac ccgaccggcg acgtcattcc cgtcactcat tgcacccaca cccctgaact
     2881 ctgccgttga cgacaagccg tccgcgctgg cttacgacaa ttgcccaccc cgggcaagcc
     2941 gttgccgagc ctgcctgcgc gaagccgaag cggcttcgct tcggcgaagg caggcgggcg
     3001 cgggctaggt agcacactcg cgccgccttt gccagccggc gccggtcaga ccatggccat
     3061 gccgccgttg atgtgcagcg tctggccggt gacgtaggcg gcctcgtcgg aggcgagata
     3121 gaccgcgccc gcggccacgt cctcgccgct gccgagccgc ccgcacggga tcgcggacgc
     3181 gagccgtgcc cgctggtccg catccagcgc ccgggtcatc gcggtgtcga tcatgccggg
     3241 agcgatgcag ttgacggtaa tcccgcgtcc cgcgacctcg gcggccagcg acttggacat
     3301 ggcgatcatg cccgccttcg ccgcggcgta gttagcctgg ccgggattgc cggtgacggc
     3361 gacgatcgag gtgacgttga tgatgcgccc gtagcgccgg cgcatcatcg gacgcagcgc
     3421 cgcgcgcgac aggcggaacg gggcggacag gttgacctcg atcaccgcct gccagtcctc
     3481 gtccttcatc cgcaccatca ggccgtcgcg ggccagcccg gcgttgttga ccaggatgtc
     3541 gagccggccg agcgccgcct cggcatcgcc cgcgagcgcg gccaccgccg ccgcgtcggc
     3601 aaggtcgcag gcgagaacat gggcgcgcgc gccaagctcg ccggcgagcg cctgaagggc
     3661 gtcgatgcgg gtgcccgaca gggcgacagc ggcgccggca cgatggagcg cgcgcgcgat
     3721 cgcgccgccg atgccgccgg acgcgccggt gacgagcgcg cccttggcgg tcaggtcgaa
     3781 catgccccgc ccctttcacc cccgggcgcg gcggctccgc ccggatcgtt cacagcgacc
     3841 tggccagcgc ttcaacgtcg tgcggtgtca gcaccgcgac accggcgagc tcggggtcga
     3901 tgcgccgggc gaggttcgcc agcaccttgc ccgcgccgag ctcgaccagc gtgtcgacat
     3961 cgtggtcctt catgtacagc acgctttccc gccagcgcac catggcggtc atctgctcga
     4021 ccaggagcgc acggatgcgg gccggctcga ccaccgcctc ggcggtgacg ttggcgacca
     4081 gcgccggtgc cggcgcggcg atcgcgatgc tgtccagcgc cgccgccatc tccgccgccg
     4141 ccggcgccag cagcgcgcaa tggaacgggg cgctgacctg caggcggacc gcgcgccggg
     4201 cgccgcgcgc gggcgcaagc tcgatcagcc ggtcgatcgc cgaggcggcg ccgctgacca
     4261 ccacctggcc gggcgcgttg tcgctggcca gcgcgcacac cgcgccgttc gccgccgccc
     4321 cggcgaggca tcgcaccttg tcgagctcga ggccgaggac cgccgccatc gcgccctcgc
     4381 ccaccggcac cgcctgttgc atggcgcggc cccgttggcg cagcaggcgc gcggtgtcgg
     4441 ccagcgacag cgccccggcg gcggcgagcg ccgagtactc gccgagcgag tggccggcga
     4501 cgaagcagcc ggcctcggcc aggcgcaggc cggcctcgtc ctcgagcacc cggaccacag
     4561 ccagcgaggt cgccatcagc gccggctggg cgttctcggt cagcgacagc tcggactcgg
     4621 ggccgtcgag gatgacccgc gacagccgct ggccgagggc ctcgtcgacc tcctcgtaga
     4681 ccaggcgcgc ggcctggaat gcgtcgcaca gctcgccgcc catgccgacg acctgcgagc
     4741 cctgcccggg aaacacgaat gcgcgcgcca tcgccccgct tttgctgccg atcgccgccg
     4801 gacccgagtt ggcgaagcgc tacaactacc gtcgccgccg gcgctgtcaa ggcgcgccgc
     4861 agcgccggcc gtgtgccgct tgcgcgcatc gaggaattgg gtatagtgcg gcgctttcgg
     4921 gcaccgggca cccatggcgg tccttccgga cccggggtcg tgccggattt gccgtcgatc
     4981 gcgggcgtcc cggagcagat ccggggttgc ccggcacgac cgaccgagga accaatcgag
     5041 gagccgctca tgccgctcta cgagagtgtg tttatcgcgc gccaggacgt gcccgcgcag
     5101 gatgtcgagg cactggccga gcgctacgcg gggattctgg ccgagcatgg cggacgggtg
     5161 gccaagcgcg agtactgggg tctgaagaac ctggccttcc ggatcaagaa gaaccgcaag
     5221 ggccattaca cgatgatgca gctcgaggcg ccggcggacg cggtccacga gatggagcgc
     5281 aacatgcgca tcagcgagga cgtgctgcgc tatctgaccg tgcgcatcga caaggtcgac
     5341 gacgagccgt cggtgatgat gcaggcccgc tcggcgcgcg acgaccgcgc gcgccgcgac
     5401 gaccggcgcg gcggacgggg cggcgagttc aggccgcggg ccgaagcgac cggcgagcgg
     5461 gccgaggccg tcaggaagga cgccggcgag gcccaggcca gcgcggatgc cgggccagtg
     5521 aaagaggaga gccgatagat ggccgacgcc aagtcctcag ccaggccggt gccgctgccc
     5581 aggcggccgt tctttcgccg ccgcaagtcg tgcccgttca gcggcgcgaa cgcgcccaag
     5641 atcgactaca aggacgtcaa gaccttgcag cggttcacct ccgagcgcgg caagatcgtc
     5701 cccagccgga tcaccgcggt ctcggcgccc aagcagcgcc ggcttgcgca ggcgatcaag
     5761 cgcgcccgct tcctggcgct gatgccctat ctgctgaagt agcgccggcc tcgccgcagg
     5821 acgacaaccg ggaacggctg acgatggaag tgatattgct cgagcgggtc gagaacctgg
     5881 gccagatggg cgacgtggtg cgggtgcgcg acggctttgc ccgcaactac ctgctgcccc
     5941 ggcagaaggc gctgcgggcg acgaagggca accgtgagcg tttcgagcgc caacgtaccc
     6001 ggctcgaggc cgacaacctg acccaccgcc aggaggccga gcaggtggcc aagcgaatgg
     6061 ccggggtgtc ggtggtcatg gtccgccagg ccggcgacac cggccagctg tacggctcgg
     6121 tgaacgcgcg cgacatcgcg gcggcggtga ccgaggccgg ctacacggtc gaccgccgcc
     6181 aggtggcgct caaccaggtg atcaagtcgc tcggcctgca tccggcccgc ctggtgctgc
     6241 accctgaggt gtcggtggag gtgaaggtca acgttgcccg ctcgcaggag gaggccaagc
     6301 tccaggccgc cggcgtcgac gtcaccgccc tcgaccgcga ggaggcggag cgcgaggcgg
     6361 cggcggccgc ggcggaggcg gaagccgcca agctgttcga ggagcaggag gacgcggcgc
     6421 gcgcagtcgt ctccgcccgc gacgaggacg agcccgccgc cggcgcgagc gaagaccagg
     6481 ccgacaagga ctaggccgcc ggccgggcgc ggccgggtgg gttcccggcc ggccgaaagg
     6541 cgctataatc cgaggcgttg cgaaccccat cggggtgcgg cgtccgccgc ttcgggcaga
     6601 cctgccctgg cggcggcccg atcgggaggc ggagatgctg ctcgagggtc tgctccgagg
     6661 cctgatccgg cacggccggc tgacggtgat ccgccatgac gggcgccaac gcaccttcgg
     6721 gtcggcgccc ggagcggagg tcgtgatccg cctgcacgac cgcgccaccg catggcgcct
     6781 ggcgctgagc ccgacgctcg ccaccgccga ggcctacatg gacgggcggc tgacgctcga
     6841 caagggcgag ctctacgacc tgctcgcgct cgccgcggcc aatatcgagc gcagcggcaa
     6901 gccgctcgtg atgcgcgtcc gcgatgcgct cagccggctg acgcgctggt tccggcagta
     6961 caacccggcg ggccgcgcca ggcgcaacgc cgcccaccac tacgacctgt cggacgagct
     7021 gtacgagctg ttcctggact cgaaccgcca gtattcctgc gcctacttca ccgacccggg
     7081 ccaggacctg gagggcgcgc agcgggccaa gatgcgccat atcgcggcca agctcctgct
     7141 tgcgccgggc cagacggtgc tcgacatcgg ctcgggctgg ggcgggctcg ggctcgagct
     7201 ggcgcgcgag gccggggtcg aggtgcgcgg cgtgaccctg tccgaggagc agcacaaggt
     7261 cgcgaccgcg cgcgcggcca aggcgggcct cggcgatcgg gtcaagttcg cgctcgcgga
     7321 ctatcgccac gaggccgggc actacgaccg catcgtctcg gtcggcatgt tcgagcatgt
     7381 cggcgtcggc cattacggcg cgttcttcgc caagctcgca gccctgctca agcccgatgg
     7441 cgtcgcgcta ttgcacacca tcgggcgctc ggacgggccg ggctcgaccg atcccttcgc
     7501 ccgcaagtac atcttcccgg gcggctacac cccggcgctg tccgaggtgg tgcgcccgat
     7561 cgagcgcgca cgcctggtga tcaccgacat cgaggtcttg aggctccact acgccgagac
     7621 cttgcgcgcc tggcgcacgc gcttcaaggc caactgggac aaggccaagg cgctctacga
     7681 cgagcgcttc tgccggatgt gggagttcta cctcgccggc agcgaggtgt cgttccgcca
     7741 tctcggcatg gtggtgttcc agatccagct cgcccaccgc caggacgcgg tgccgctgac
     7801 gcgcgactac gtcaccgacc acgaccgcag ccccgacgcc aaggacagcg tcgccgcgga
     7861 atagcgcgcc cgcccgatcg aaggccgacc gacgtcgcga tgtttcaacc ggggcggaac
     7921 ggggccaggc acatccacac gccgtcccga aaccttgact gggcggaacg gcccgtgcag
     7981 cgtcgccgtg cctagagcct gatgatttga aacatggtca tccatcgccc gtcaccccgg
     8041 ccaaggcgcg cagcgccgcg agccagggtc catcccgcca cttgcccggg tggtcgagcg
     8101 cgtggcaaag gcgtatggac cccggatatc cgcttcgcgg agtccggggt gacggccaaa
     8161 acaggctcag ggatccgact taatatcatc ccgctctagg cgggtgagcg gcgcgggcgg
     8221 cggccggctt tgccggtggc cgagtcgccc cagcgggtga cggtgccggc atcgagcccg
     8281 aacaggtcga gcacccgccc cagcgtgtgg tcgatcatct cctcgaggct ctgcgggcgg
     8341 gcatagaacg ccggcaccgg cggcgcgatc accacgccga gctcggcaag ccgggtcatg
     8401 ttggcgaggt ggatggcgtg cagcggcgtc tcgcgcacca tcagcaccag cggccggcgc
     8461 tccttgagca ccacgtcggc ggcgcgggtg agcaagttgg cgcacaggcc ggtggcgatc
     8521 tcggacatgc agcgcaccga gcacggcgcg acgatcatgc cgagcgtcgg gtacgagccg
     8581 ctcgacagcg acgccgccag gtcgtcgacc gggtgccagg cgtcggcgag cgcccgcacc
     8641 tcggcgagct tgcggtcggt ctcgtgggcg agcgtcacct cggccgagtg gctcatcacc
     8701 aggtggctct cgaccgcgca cgccctcaac agctcgagca ggcgcacccc gtagatcgcg
     8761 cccgaggcgc cgctgatgcc gacgatcagg cgcgccggcg ggtcgtgatc ggtgcccgcc
     8821 accggcgtca accggcaatg cgctcgatcg tgctggtggt gctgtatccg ggcgtgattt
     8881 cggccaggag cacgtcgccg ccgtagcttt tcacgaactc cgcccccacc acctcggaca
     8941 gcaagtagtc ggcgcctttg accagtacgt cgggccggaa cagcctgaga agccgcatcg
     9001 gcgtgtcgtc ctcgaacacg accaccaggt cgatgtgctc gagcgcggcg agcagcatcg
     9061 cccgcgccgc ctcgggctgg atgggtcggc cggggccctt gatccgcgcc accgaggcgt
     9121 cgctgttaag gccgaccacc agccggtcac aggcggcgcg cgcctggcga agcagcgaga
     9181 tgtgccccgg gtgcaaaagg tcgaagcagc cgttggtaaa gccgatcttc tggccctgcc
     9241 gccgccacat ggcgattcgc ttggcggcgg gctccatggc cagcaccttg gtgtcggcgg
     9301 cgcgtacgct gccgtggcgc aaggcttgcc tgagctcgtc agccttgacc gcggcggtgc
     9361 cgaccttgcc gacgacgata ccggcggcga agttggccag ccgggcggca tcgagcatgt
     9421 cgattccggc ggcgattgcg gcggtgaaag tcgccgccac ggtatctccc gctcccgaca
     9481 tatccaagac ctcctgggtg tgcgcgggga tgtgctcggt gtgactgaag tcaaccaggc
     9541 tcatcccgtt gcgtccgcgc gtcaccagca ctctctggat gccacaacgc tggatcaccc
     9601 ggcgcgccgc caccaccgcg ctggtgtcgt catcgatctt ggcccccgcc tcggcgccga
     9661 gctcgagcca atttggcgtg agaacgtccg cgcctcgata ccggttgaag tcgagcccct
     9721 tggggtcgac gaccaccggt cgcgcgcacc ggcgcgcgca ctccacgacc gcctggacga
     9781 ccggtccgtc gaggacgcct ttgccgtaat cggacaggac tacggcgtcg caggccggca
     9841 cggcctcggc ggcgaggcgt gagacttcgg cggtgacttg actgggcaat ggcgccaaag
     9901 ggtcgcgatc ggcacgtaac agttgttgcc cgtcagcgac gtaacggctc ttgacgatcg
     9961 atccgtgctc cgagtcgacg agcagatgcg gctcgacggc agggctgctg gtcagcagcg
    10021 cctccagctc acgtccaacc ggatcgccgc cgatgaccga gacaaaggtg cagcgggcgc
    10081 cgagcgagac caggttgcgg accacgttgc cggcgccgcc caacatggtg gcctccgcca
    10141 cgatccgcat gaccggcgcc ggcccctccg gcgacatgcg ctcgacggtg ccgtagacgt
    10201 atctgtcgag catcaggtcg ccgacgcaca gcacccgcac cgcgcccagc cgctcgacat
    10261 cgccggcaag gttcgcgagc tcggtcatgc cccggatgcc cggtccggca accgtgccgg
    10321 ctcagccgcc ctgcgagtcc gccgggccgg cggcggcgcc gtcctgcgcg gcgtccgccg
    10381 cctgctcgcg tgccaggcgg cggcgcgatc gcgccagctg ctcctccagc tcgccctggc
    10441 tgcagatgcc gagcagcacc ggatcgcgcg gctggatgtt gctgatgttc cagtggctgc
    10501 ggctgcgcac cgcctcgatg gtcggcttgg tggtgccgac cagcttgcag atctgcgcgt
    10561 cggtcaactc cggcgtgtat ttgagcagcc aggcaatccc gtccggccgg tcgccgcggc
    10621 gcgagaccgg cgtgtagcgc gggcccttgc gcttgggcgc cgccgcgacc tcggcgctga
    10681 gcagcttcag ccgggccttg gggtcggcct cgcagcgctt gatgtcggcg cgcgacacct
    10741 ggccgttgcc gaccggatcc aggccgacga tgccgaccgc gacctcgtcg tcggcgatcc
    10801 cctggacctc gagcgggtgc aggccgcaga agtctgcgat ctggtcgaag gtcagcgtcg
    10861 tgttctcgac cagccatacg gccgtggcct tcggcatcaa tggctgcgcc atcatcggtc
    10921 ctcgttgtca ggtcattgcg cgccgccgcc cggggcatcg agggcgcgcc acgcagccga
    10981 tcttgggcgc ccacggggcg cccgccgact atagtcgacc ttgcgcgtgc gcgaaaggcg
    11041 cggctgcacc tagaccgcga gcacgatctt gccgacgtgg cggcccgctt ccatcagcgc
    11101 atgcgcctcg gccgcggacg tgagcgggaa ggtccggtgg atcaccggcc tcacccgccc
    11161 cgattccagc agcggccaga catgcgccct gagcgcttcg gcgatctggg acttgcgttc
    11221 gaccgtgcgc ggcctgagcg cgctgccggt gacggtcagc cggcggcgca ggatcggcat
    11281 caggtcgacc tctgcggcac tgccgccgag gaaggcgatg atcaccagcc ggccgtcgtc
    11341 ggcgagcgcg gcgaggttgc gcttgagata gtcgccgccg accatgtcga ggatgacgtc
    11401 gacgccgcgg ccggcggtcg cgtccttgac cacggcgagg aagtcctcgc tccggtagtc
    11461 gatcgcgcgc tcggcgccga gcgactcgca ggcgcggcac ttctcgggcg agccggcggt
    11521 ggcgaagacg cgggcgccga acgcgtcggc gagctggacc gcggtggtgc cgatgccgct
    11581 cgagccgcca tggaccagca cgctctcgcc gccgtcgagg cggccgcgct cgaacaggtt
    11641 ggtccacacg gtgaaatagg tctcgggcag cgccgccgcc tggaccgcat cgagcccggc
    11701 cgggatcggc agcgtctggt cggcgggcgc gacgcagaac tcggcgtagc cgccgcccgt
    11761 gacgagcgcg cacaccgcct cgcccggggc gggcccggca acgcccgcgc cggcgcgcac
    11821 cacggtgccc gcgacctcga gcccgggcag gtccgaggcc ccgggcgggg gcggatagct
    11881 gcccgcgcgc tgcatcacgt cggggcggtt gacccccgcc gcggcaaccc ggatcagcac
    11941 ctcgccgtgg cccggcaccg gcaccggacg cgtgaccgcg cgcagcacct cgggcccgcc
    12001 gggagcggag atctcgaccg cggtcatggt ctctggcacg gcgccggtca cggcgtcagc
    12061 accatcccct cgcgcgccac caacgtgccg gcgcgcgcct tctcggcctc gcccgcgatc
    12121 ctgtccatga agtcgtcgtc atggtcggga ttgtgatgga acacgaccag ggtcttggtt
    12181 ttggcctcgt cggcgaggcg caccccctcc tgccaggtcg agtggcccca ctcgaggtgg
    12241 tcggcgaact cgtcgtcggt gtaggtgctg tcgtagatga acaggtcggc gccctcgatc
    12301 aggccgagga tgttgcggtc gggcttgccc ggcacgtgct cggtgtcggt gacgtagcag
    12361 atcgagcggc cgccgtactc gatccggtag ccggtggcgc ggttgggatg gttgagcggc
    12421 gccgtgcgca cgaccacgcc gtcatgcggc tgaagcgcct cgccggcgac gaagtcgtgg
    12481 aagtcgagct ccgcgtgcat ggtgtcgatc gggatcggga acagcgggtc catcatcagg
    12541 tagatcagca cctcgcgcac ggtgcgctcg ggcaacaggt gcccggccca gatcgacagc
    12601 cggttctcgg ccgcgaacgc gggcgcgaag aacgggaagc cgcagatgtg gtcgaaatgg
    12661 gtatgggtga agaacaggtc ggcggtgaac ggcccgtcat gcgggcggtt gcgcacgatg
    12721 tcggtgccga gcgcgtgaat cccggtgccg gcgtcgagca ccagcagccg cccgtcgcaa
    12781 ttcacctcca ggcacgaggt gttgccgcca tagcgcatgg tgccgggccc cgggcacgcg
    12841 atgctgcccc tgacccccca gaagcgaacg aagaagtcgg gtgtctccgg caagtctcgc
    12901 tctccgcggg tctccgttcc gccgccggcg ctgaccgccg atctggaccg cccccgcgcg
    12961 gccggcgggc ggttgctccg agctcgggcc tcgtccgttt gtgacgtttc gacgatacgc
    13021 gaaccgcccc ttattgcaac cgggatgttt tcgcctaagc tgtgcgctcg agcgccgagc
    13081 gccgcgcctc gaacggcgcg cgccggcgcg gttgcgaggg atgccggatg ttcgacgacg
    13141 acgaccgccc cgcccggccc gcgcgcacca ttccgcgcaa cctggacaag atgtcgatcg
    13201 ccgcgctcga ggactacatc gccgagctcg aaggcgagat cgccagggtc aggcaggaca
    13261 tcagtgccaa gcaacgcctg cagtccgaag ccgagaaggt cttcaagagc ggccggtgac
    13321 ctgcggcgac cgctaaaatt tgttgcaatt tgcttacgaa agtttggcgg tcgtcttgcc
    13381 ggcgcaacca attacgctag attgctcaag tggtagaacg cggccggaga ttcgtggatt
    13441 tgccgaccgt gtcatgctga tgacctccct gtggacttac tgggccgccc gaaccaattc
    13501 ggcggccctt ttgtttgcgc tgcccgcaac ccgaatccga accgcccggc gaccgctgga
    13561 ccgggcgacg ggctgactgt aggctcgccg ccgctgcccg aacccgtccc gattcgcgga
    13621 gcgagagatg gccgaagcaa agttcctcaa cgccgagacg ctcatgctgc acgccggcca
    13681 gcatccggac cccgagttcg gcgcgcgcgc ggtgccgatc ttccagacca cgtcctacgt
    13741 gtttcccgac accgacaccg ccgcctcgat gttcaacctc gagcgcgccg ggcacctgta
    13801 ctcgcgcatc agcaacccga cggtggcggt gctcgaggag cgcctgagcg cgctcgagcg
    13861 cggggtcggc ggcgtctgca cggcgagcgg ccaggcctcg ctccatctcg ccgtcgcgac
    13921 cctgatgaat gcgggcggcc acatcgtcgc ctcgaagtcg atctacggcg gcagctacaa
    13981 catgatgggc cacaccttgc cgcgcttcgg cattaccacc accttcgtcg acgcgcgcga
    14041 tcctgcggcg ttccgcgacg cgatccggcc cgagacccgg ctgttgttcg gcgagacgct
    14101 cggcaacccg gggctcgagg tgatggatat cgcggcggtc gccgagatcg cccacgccgc
    14161 gcggctgccg ctgctgatcg acaacacctt cgccaccccc catctgtgcc ggccgttcga
    14221 gttcggctgc gacctggtga tgcactcggc gaccaagttc ctgggcggcc acggcatcgc
    14281 catcggcggc gcgctcatcg acagcggccg gttcgactgg gagcaatcgg gtaagttccc
    14341 gaccatgacc gagccctacg agggctatca cggcctcgac ttcgccgagg agttcgggcc
    14401 ggcggcgatg atcatgcgcg cgcgcgccga gggcctgcgc gacttcggcg gctgcatgag
    14461 cccgcagaac gcgttctata tcctgctcgg ggtcgagacc ttggcggtgc gcatggagcg
    14521 ccacgtcgcc aacgcgcgca aggtggtcga gtatctgtgc ggccacgacg cggtcgaatg
    14581 ggtgacctgg cccgagcttc ccgagcaccc cgattacgag ctggccaaac gcatgctgcc
    14641 gaagggttgc ggtgccatct tttcgttcgg gatcaagggc ggccgcgccg ccgggcgcag
    14701 cttcatcgag cggctgaagc tgttctcgca cctggccaat gtcggcgacg ccaagtcgct
    14761 ggtgatccac ccggccagca ccacccacca gcagatgagc gcggccgagc ttaaggccgc
    14821 gggcgtcggc gaggagctgg tgcggctgtc ggtcgggctc gaggacccgg gcgacctgat
    14881 cgacgacctc ggccaagcgc tgcgcgcctc gcagcgggct tgagcgatgg agctcgaggt
    14941 cgcaggcgcg ccggtgcacg ccgccaccgg cggcgtcgcg ctcgatcccg cccggccgct
    15001 gatcgtgttc gtccacggcg ccggcatgga ccacacggtc tgggcgctgc aggcgcgcta
    15061 cttcgcccat aacggctatg gcgtgctggc ggtcgacctg cccggacacg ggcgcagcgc
    15121 cggcgaggcg ctcgactcga tcgaggcgct cggccgctgg ctgtggcggc tggtcgatgc
    15181 cgccgggcct gagcacgcgc gcctggtcgg ccacagcatg ggcgcgctcg ccgcgctcga
    15241 ggcggcggcg gcggcgccgg cccgcgcgcg cggcctggtg ctgatcggcg ccaatgcggc
    15301 gatgccggtc aacgacgagc tgctgtcggc ggcgcgcgag gagcccgcgc gggcggcggc
    15361 actgatcgtc tcctgggcgt atggcgagcg cagccatatg ggcggcatgc gcgcgcccgg
    15421 gctgtggatg ctgggcggcg gcctgagcct gctcgcgcgc gcgcgagccg gtgtgctgca
    15481 cgcggacttc gcggcctgca acagctaccg gggcgggctc gccgcggcgg cgcgcgtgtc
    15541 ctgcccgacc ttggtgctgg cggggcgcgg cgaccgcatg acgcccgcgc gcggcgccgc
    15601 caagctcgcc gaggcgatcc ccggcgcgcg cctcgccgtg atcgaggacg ccggccacat
    15661 gccgatgatc gagcagcccg accgcacgct ggacgcgctc agggcgcagt tcgccgcgcc
    15721 gtgaccgaga ccgaagcgcg gcgccgcacc gactatgcct acctgcgcgc cgtgccgacg
    15781 cgctggaacg acaacgacct ctacggccac ctcaacaacg tcgcctacta cgagatcttc
    15841 gataccgtca tcaaccgctt cctaatcgag gaaggcggcc tcgacatcgc ccgcgggccg
    15901 gtgatcggga tcgtgcccga gacccgctgc cgctacctca agccgctgcg ctatcccgac
    15961 gtgatcgacg tcgggctcgc ggtcgccagg ctcgggcgga ccagcgtggt ctacgacatc
    16021 gccctgttcc gccagggcga ggacgatgcg gccgcgctcg gccacttcgt gcacgtgttc
    16081 gtcgcccgcg ccgcgcagga ccggacccgg ccgatcccgc gccgcgtgcg gagtgcgctg
    16141 gcgcgcatcg cgcgcgcgcc ggaggaagat tgagacggct acttcttgcc caggccgaag
    16201 ccggcgaagc gcttgttgaa gcgcgccacc tggccctcgg tatcgaccag gcgctgggcg
    16261 ccgccggtcc aggccggatg gctgagggag tcgacgtcga gcttcatcac gtcgccttcc
    16321 ttgccccagg tagtgcgggt cttgaactcg ctgccgtcgg tcatcaccac ggtgatctcg
    16381 tggtaatcgg gatggatgtc cgtcttcatc gcctggctct cctgtcgctc ggcgccgtat
    16441 atatccaacc acgtgcgccg gtacaagacg gggcacgcgg cggcgcggag ggcgcgggca
    16501 gggcgcgcgc cggcgccggc cgggacgata ggtaggatta ccgcgtctcc gcgcctcggc
    16561 gtgagcccaa cgaccgccct gtggcacctc gggccgggcc gatatacggt gcgcgggatg
    16621 accgaatgcc ctgatcggcg cccgcgatga cgctcgcgga tgcgcgcgcg agcgaccgcc
    16681 cgcgcagccg cgagctgcgc cacctggcca agctcgccgg ctacctgctg ccctaccgct
    16741 gggtgatcgc cggcgcgatc ggcgcggtcg cggtggccgc cgccaccgtg ctgtcgctcg
    16801 gcatcggctt gcggctgctg gtcgacgagg gcttcggcgg cggcgatccg gcgctgctcg
    16861 accgcgcgct ggtcgcgctg gtcgcggcgg tggtggtgct ggcgagcgcg acctatggcc
    16921 ggttctacct ggtcagctgg atcggcgagc gggtgatcgc cgacatccgc cgcgacgtct
    16981 ataaccacgt catcgccctg tcgccggcct tcttcgagac cctgcgcacc ggcgaggtga
    17041 tgtcgcggct gaccgcggac accacgctgt tgcaggtggt gatcggatcg agcgcctcgg
    17101 tggcgctgcg caacctgctg ttgttcgcgg gcggcagcgt gatgctggcg gtgaccagcg
    17161 ccaagctgac cctgatcacg gtggtgctgg tgccgctggt ggtgctgccg atcgtggtct
    17221 tcggccggcg ggtgcggcgc ctgtcgcgca ccgcccagga ccgtgtcgcc gacgtcagcg
    17281 cccaggtcga ggaatcggtc gcggcgatcc gcaccgtcca ggccttcacc cacgaggcgc
    17341 acgaccgcgc ccgcttcgcc ggccatgtcg agagcgcgtt cggcgccgcg gtggcgcgcg
    17401 tgcgcgcgcg cgcgctgctg accgcgctgg tcatgtgcag caccttcggc ggggtggcgc
    17461 tggtgctgtg gatcggcggc cgcgacgtgc tggcgggcgg gctgtcggcg ggggagctat
    17521 cgcagttcgt gttcttcgcg gtggtggtcg ccggcgccct cggcgcgatc agcgaggtct
    17581 atggcgacct ccagcgcgcc gccggcgcca ccgagcgcct gctcgagctg ctcgatgcgc
    17641 gctccgaggt cgcggcgccg gcgcatccgg tgccgctgcc caagccggca cagggcgcga
    17701 tcgcctttca ggcggtgacg ttcttctacc cgagccgccc cgacgccgcc tcgctcacgg
    17761 agctcacgct cgacatcgcg cccggcgagc g
//
LOCUS       DASWHU010000293         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2033022, whole genome shotgun sequence.
ACCESSION   DASWHU010000293 DASWHU010000000
VERSION     DASWHU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033022"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..3433
                     /locus_tag="VGB88_10960"
     CDS             320..3433
                     /locus_tag="VGB88_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9648010.1"
                     /translation="MLRWIVRASVNHAGVVALLAVLLLAYGGYRLAIASLDIFPEFSP
                     KLVIIQTEAPGFAAEQVELLVSQPIESALGGLIDVTEVRSQSIQGLSVVNVVFDDAIA
                     LLRARQVVAERLSRLATEMPAGVAAPTMVPLTSSSSTVMTIGLTSASLSQGELRSLAD
                     WTLKPRLLRVAGVADVNVFGGEVRELQIQARVGDLRRHRVALAELEAAAHSAVDLRGA
                     GFVQTPTQQITLRTTGQTVAAGELAGVVVRPGRPAIRIGDVADVIDGAAPAISKASVL
                     AEPGVVIMIIGQFGANTLAVSRNVDAALAEVRPVLERQGVSLRDDLFRPANYIERSLA
                     GIAQHLAVGAVMVIVVLVLFLFNLRSAFISAMAIPLSLVTATLVLLEAGVNLNIMVLG
                     GLAIALGEVVDDAIIDTENIFRRLRENRRLAQPRPFRAVVFDASMEVRGSVVYASFIV
                     GLAFVPLLSLSGAAGRMFEPVGVTYILAILASLAVALTVTPALCVLLLTGKRLSLRDP
                     PLMRLLQPAYGALLRAIGRHDRLVMALVAVGLAIGLGVLPYLGSRFLPELREGHYMIH
                     TSGVPGTSLDETIRTGARLTEAVLQVPGVRAMSQWAGRAERGADTYGSHYAEYEVDLE
                     RLSGREQQRVLDTIRGILDRFPGIVSEINTFLIERVDETISGYTAAVVVNLYGADLDF
                     LDRKGQEIAALLRAVPGAVDVQIQAPHGVPILAVELDEQRLALHGIRPGDVYDVIQTV
                     YEGSAVGALHDGDRVLPLVVTVTPELRTRPEAAAELPVRAASGEVIALGDVADLRPDS
                     GRYAILHEGGRRLQTVTANVAGRDFGSFVAELQRRLNDDIALPIDSYIEITGSAVAAA
                     QSRRELIIHSALAGIGVLLLLQLALGRAWNVALVVTNLPFSLVGGVVAALVTGGVLSL
                     GSIVGFITLFGITLRNAIMLVSHYQHLVEIDGEPWTMDTAVRGAKERLPSILMTALVT
                     ALAMLPIAVDSDNPGREIMGPMASVIIGGMASSTLLNLLVLPSIMYRYGRFGARTGLV
                     GEAAAAR"
BASE COUNT          444 a         1204 c         1234 g          559 t
ORIGIN      
        1 ctcgaccacg cgggcgcgga cggcgtgtac gtggtcgaca ccctcgccga ggacgacgag
       61 gtggtggtca gcggcgcggt cgcggtctac gccgaggagt tccgctcgca gatccgcaac
      121 gaggacgact gactatgtac cgcccctgcg cgcacggccc cctcaccctt ccctctcctc
      181 ccactcgtgg ggggagaggg ttgcgaaggg ttggcgcggc gcacgccgcg ccctacccgg
      241 tccccggatt aaatccgggg atgggtgagg ggggcgcgat ccatcggcgg cggataaggt
      301 tctagacggg gctccggcga tgctgcgctg gatcgtccgg gcctcggtca accacgccgg
      361 cgtcgtcgcc ctgctcgcgg tgctgctgct cgcctacggc ggctaccggt tggccattgc
      421 cagcctcgac atcttccccg agttctcgcc caagctggtg atcatccaga ccgaggcgcc
      481 gggcttcgcc gccgagcagg tcgagctgct ggtctcgcag ccgatcgaaa gcgcgctcgg
      541 cggcctgatc gacgtaaccg aggtgcgctc gcagtcgatc cagggcctgt cggtggtcaa
      601 cgtggtgttc gacgacgcca tcgcgctgct gcgcgcgcgc caggtggtcg ccgagcgcct
      661 gtcgcgcctc gccaccgaga tgccggcggg ggtcgccgcg ccgaccatgg tgccgctcac
      721 ctcgtcgtcg tcgacggtga tgaccatcgg gctgacctcg gccagcctga gccagggcga
      781 gctgcgcagc ctcgccgact ggaccttgaa gccgcgcctg ctcagggtcg ccggcgtcgc
      841 cgacgtcaac gtgttcggcg gcgaggtgcg cgagctgcag atccaggcgc gcgtcggcga
      901 cctgcgccgc catcgcgtgg cgctggcgga gctcgaggcc gcggcgcatt cggcggtcga
      961 cttgcgcggc gccggcttcg tccagacgcc gacccagcag atcaccttgc gcaccaccgg
     1021 ccagacggtg gccgcgggcg agcttgccgg cgtcgtcgtc cgccccggcc ggccggcgat
     1081 ccggatcggc gacgtggccg acgtcatcga cggcgcggca cccgcgatca gcaaggcgag
     1141 cgtgctcgcc gagcccggcg tggtgatcat gatcatcggc cagttcggcg ccaacacgct
     1201 ggccgtcagc cgcaacgtcg acgcggcgct cgccgaggtg agaccggtgc tcgagcgcca
     1261 gggcgtgagc ttgcgcgacg acctgttccg gccggccaac tacatcgagc gctcgctcgc
     1321 cggcatcgcc cagcacctgg cggtgggcgc ggtgatggtg atcgtggtgc tggtgctgtt
     1381 cctgttcaac ctgcgcagcg cattcatctc ggcgatggcg atcccgctgt cgctggtcac
     1441 cgcgacgctg gtgctgctcg aggccggggt caacctcaac atcatggtgc tgggcgggct
     1501 cgcgatcgcg ctcggcgagg tggtcgacga cgcgatcatc gataccgaga acatcttccg
     1561 ccggctgcgc gagaaccgcc ggctggcgca gccgcgcccg ttccgcgccg tggtgttcga
     1621 cgcctcgatg gaggtgcgcg gctcggtggt ctatgcgagc ttcatcgtcg ggctagcctt
     1681 cgtgccgctg ttgtcgctgt cgggcgcggc ggggcgcatg ttcgagccgg tcggggttac
     1741 ctacatcctc gccatcctcg cctcgttggc ggtggcgctg accgtgaccc cggcattgtg
     1801 cgtgctgctg ctgaccggca agcggctgtc gttgcgcgat ccgccgctga tgcggctgtt
     1861 gcagccggcc tacggcgcgc tgctgcgcgc gatcggccgc cacgaccggc tggtgatggc
     1921 gctggtcgcg gtcgggctcg cgatcgggct cggcgtcctg ccctatctcg gcagccgctt
     1981 cctgcccgag ctgcgcgagg gccattacat gatccacacc tcgggcgtgc ccggcacctc
     2041 gttggacgag accatccgca ccggcgcccg cctgaccgag gcggtgctcc aggtgcccgg
     2101 ggtgcgcgcg atgtcgcaat gggccgggcg cgccgagcgc ggcgccgaca cctatggcag
     2161 ccattacgcc gagtacgagg tcgacctcga gcgcctgtcg gggcgcgagc agcagcgcgt
     2221 gctcgacacc attcgcggca tcctcgaccg cttcccgggc atcgtctcgg agatcaacac
     2281 cttcctgatc gagcgcgtgg acgagaccat ctcgggctat accgcggcgg tggtggtcaa
     2341 cctgtacggc gccgacctcg acttcctcga ccgcaagggc caggagatcg ccgccctgct
     2401 gcgcgccgtc ccgggcgcgg tcgacgtgca aatccaggcg ccgcacgggg tgccgatcct
     2461 ggcggtcgag ctcgacgagc aacgattagc gctgcatggg atccgtccgg gcgacgtcta
     2521 cgacgtgatt cagaccgtct acgagggcag cgcggtcggc gcgctccacg acggcgaccg
     2581 ggtgctgccg ctggtggtca cggtcacgcc cgagctgcgc acgcgccccg aggccgcggc
     2641 ggagctgccg gtgcgcgccg cgtccggcga ggtgatcgcg ctcggcgacg tcgccgacct
     2701 ccgtcccgat tccggccgtt acgcgatcct gcacgaaggc gggcggcggt tgcagacggt
     2761 caccgccaac gtggccgggc gcgacttcgg ctcgttcgtc gccgagctgc aacgccgcct
     2821 caacgacgac atcgcgctgc cgatcgactc ctatatcgag atcacgggct cggcggtggc
     2881 ggcggcgcaa tcgcgccgcg agctgatcat ccactcggcg ctcgccggca tcggcgtact
     2941 gctgctgttg cagcttgctt tggggcgggc gtggaacgtc gcgctggtgg tcaccaacct
     3001 gccgttctcc ctggtcggcg gcgtggtcgc ggcgctggtc acggggggcg tgctgtcgct
     3061 cggctcgatc gtcggcttca tcaccctgtt cgggatcacg ctcaggaacg cgatcatgct
     3121 ggtctcgcac taccagcacc tggtcgagat cgacggcgag ccgtggacca tggacaccgc
     3181 cgtgcgcggg gcgaaggagc ggctgccgtc gatcctgatg accgcgctgg tcaccgcgct
     3241 cgcgatgctg ccgatcgcgg tcgacagcga caacccgggg cgcgagatca tgggcccgat
     3301 ggcgagcgtg atcatcggcg gcatggcgtc gtcgacattg ctcaacctcc tggtgctgcc
     3361 ctcgatcatg taccgctacg gccgcttcgg agcgcgcacc gggctggtcg gcgaggccgc
     3421 ggcggcgcga tgagcgagac g
//
LOCUS       DASWHU010000294         5055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_161898, whole genome shotgun sequence.
ACCESSION   DASWHU010000294 DASWHU010000000
VERSION     DASWHU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161898"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="lpxB"
                     /locus_tag="VGB88_10965"
     CDS             complement(<1..915)
                     /gene="lpxB"
                     /locus_tag="VGB88_10965"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9648011.1"
                     /translation="MASPAEPAPAARSPLVFVVAGEPSGDALGARLMAALARATGARV
                     RFAGVGGRAMTACGLDSLFPMSELSLMGLAEIIPHIPRVLRRLRETAAAVARLRPDVV
                     VTIDSPGFSFRLARRIRALGVPMVHYVAPQVWAWRPKRAQTLAGLFDRLIAVLPFEPA
                     FFAEFGVRCSYVGHPVVEGGFDAGDGARFRARHGLAPETSLVAVLPGSRRGEVDRLLP
                     IYGRALALLTPRHAGLCAVVPTTDTVAARLGAAVARWPVPAIVVGDPAEHADAFAASR
                     AAITKSGTSTLQLALAGVPMVVCYRVNPL"
     gene            complement(899..1882)
                     /locus_tag="VGB88_10970"
     CDS             complement(899..1882)
                     /locus_tag="VGB88_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9648012.1"
                     /translation="MAGRIRTAVVGVGHFGARHAEKYAAHGGADLAFVVDSDRARAAD
                     VGARLGATATTELGDLVGAVDAASVAVPTPHHYAVARTLLEHGIHVLVEKPITDRLEL
                     AEDLIVLAEARGLVLQVGHIERFSPAFEALRSRVRRPLYIECTRVSAFSGRSTEIDVV
                     LDVMIHDIDLVLALVKARLESVDAVGAPVLGSRDDIANTRLMFDNGCVANVTASRVSN
                     KTERKMRVFQADAYIVADFAGGKLVHVRKGKGEMMPGIPAIEREEHDIGTADALEREI
                     AAFLDSVATGAPVRVTGRDGAEALAAALLVGTSLTDHRKRLSGDVGLGVAG"
     gene            1972..3024
                     /locus_tag="VGB88_10975"
     CDS             1972..3024
                     /locus_tag="VGB88_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9648013.1"
                     /translation="MATFDSPDFDDHEAVHWFNDTRSGLRAIVAIHKRRGGRAGGGAR
                     MMAYPDEQAALNDALRLSRAMTYKHVLCGSPFGGGKIIVLGDPARAKTDAKFRALGRI
                     IDSFAGGYVTGEDVGTTPRDMALIREGTRWVIGVEGAGGDTSPATAYGVQLGIEAAVR
                     RALGATSVEGLTIAVQGVGNVGFALARRLAEAGARLVAADADPARAQRAAAELGARIV
                     APEQILAVEAEVLSPCALGGVLNDATIPRIRAKVVAGAANNQLAEARHGAMLAERGIL
                     YAPDYVINAGGVLHALVELEGFDPARVFRDVRRIREALEEVFARAVRTGEPTSAAADA
                     IARERLAAEADGAQAA"
     gene            complement(3079..4212)
                     /locus_tag="VGB88_10980"
     CDS             complement(3079..4212)
                     /locus_tag="VGB88_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9648014.1"
                     /translation="MRRVQEHKFIDLAKPVERKTDQAIDRRSFLAAATAIAGITAMSS
                     SAFARNFGPDDEPVRYPEPDVMGLDPRFKYKLGNTPILRLYRGTLWAEGCAWNGVGRY
                     LIWSDIPENMQLRWIEEDGHVSKMFRYPSGNSNGNTFDYQGRQIACEHGNRRVVRYEH
                     DGSMSVIADSADGKELNAPNDVVVHPDDGSIWFTDPGYGGLMNYEGHRLGTGSVQPIQ
                     KEATYRVDAKTGKMTKVADEPFKPNGLCFSNDYKKMYIADTGASHYKEAKNIIWGYDV
                     DGATLKNPRTFADMELDGRAGFADGIRADEDGNVWSSAGWVGDGYDGVHIFAPNGDRI
                     GQIRMPEIISNVCFGGSKKNRLFMTGSQSLYAVYVETRGAHIT"
     gene            complement(4459..>5055)
                     /locus_tag="VGB88_10985"
     CDS             complement(4459..>5055)
                     /locus_tag="VGB88_10985"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEX9648015.1"
                     /translation="LSRLVVLDVDPGHGGADSIAAWERRHGPMPETVECTTGGGGRHL
                     YFGHPGGEVHNRAGLAPGIDLRGDGGLVVAPPSIHPSGRRYRWRAGRDPDRRALAPMP
                     AWLLRVVLREGARRGHPTAYWRSLVEAGVAEGRRNDTAASLVGHLLWHGVDPEVALEL
                     LLCWNRVKCRPPLPDSEVAEVVAGIARLHAREQGEDGP"
BASE COUNT          728 a         1813 c         1823 g          691 t
ORIGIN      
        1 cagcgggttg acccggtagc acaccaccat cggcacgccg gcgagcgcga gctgcaaggt
       61 gctggtgcct gacttggtga tcgccgcgcg gctggcggcg aaggcatcgg cgtgctcggc
      121 cggatcgccg accacgatcg cgggcaccgg ccagcgcgcc accgccgcac cgagccgcgc
      181 ggcgacggta tcggtggtcg gcaccaccgc gcacagcccc gcatggcgcg gcgtgagcaa
      241 agccagcgcg cggccgtaga tcggcagcag ccggtcgacc tcgccccggc ggctgccggg
      301 cagcaccgcg accaacgaag tctcgggggc aaggccgtgg cgggcgcgga accgcgcgcc
      361 gtcgccggca tcgaagccgc cctcgaccac gggatggccg acataactgc agcggacgcc
      421 gaactcggcg aagaacgcgg gctcgaacgg caacaccgcg atcaggcggt cgaacaggcc
      481 ggcgagcgtc tgcgcccgct tcggccgcca ggcccagacc tggggcgcca cgtaatgcac
      541 catcggcacg ccgagggcgc gaatccggcg cgcgagccgg aacgagaagc cgggcgaatc
      601 gatggtgacg acgacgtcgg gccgcaggcg cgccaccgcc gccgcggtct cgcgcaggcg
      661 ccgcagcaca cgcgggatgt gcgggatgat ctcggccagg cccatcagcg acagctcgga
      721 catggggaac aggctgtcga gcccgcacgc cgtcatcgcc cgcccgccga cgccggcgaa
      781 gcggacgcgc gcacccgttg cgcgcgcgag cgcggccatc aggcgcgcgc cgagcgcgtc
      841 gcccgagggc tcgccggcga ccacgaacac caacggcgag cgtgcggcgg gcgcgggctc
      901 agccggcgac gccaaggccg acatcgcctg acaggcgttt gcggtggtcg gtgaggctgg
      961 tgccgaccag cagcgccgcc gccagcgcct cggcgccgtc gcgcccggtc acccgcaccg
     1021 gcgccccggt cgccaccgag tcgagaaagg cggcgatctc gcgctcgagt gcatcggcgg
     1081 tgccgatgtc gtgctcctcg cgctcgatcg cgggaatccc gggcatcatc tcgcccttgc
     1141 ccttgcgcac gtggaccagc ttgccgccgg cgaagtcggc gacgatatag gcgtcggcct
     1201 ggaacacgcg catcttgcgc tcggtcttgt tgctgacccg gctggcggtg acgttggcga
     1261 cgcagccgtt gtcgaacatc agccgggtgt tggcgatgtc gtcccggctc cccagcaccg
     1321 gcgcgccgac tgcatcgacc gactcgagcc gcgccttaac cagcgccagc accaggtcga
     1381 tgtcgtggat catgacgtcg agcaccacgt cgatctcggt gctgcgcccg ctgaacgccg
     1441 agacgcgcgt gcactcgatg tagagcggac ggcggacgcg gctgcgcagc gcctcgaacg
     1501 ccggcgagaa gcgctcgata tggccgacct ggagcaccag cccgcgcgcc tcggcgagca
     1561 cgatcaggtc ctcggcgagc tcgagccgat cggtgatcgg cttctcgacc aggacgtgga
     1621 tgccgtgctc gagcaaggtg cgggcgacgg cgtaatggtg gggggtgggc accgcgacgc
     1681 tcgccgcgtc gaccgcgccg accaggtcgc cgagctcggt ggtcgccgtg gcgccgaggc
     1741 gggcgccgac gtccgcggcg cgcgcccggt cgctgtcgac cacgaaggcg aggtcggcgc
     1801 cgccatgcgc ggcatatttc tcggcgtggc gggcgccgaa atggccgacg ccgacgaccg
     1861 cggtgcgaat cctgcctgcc attatccagc cctgttgccg acgcgcattg tagccggtcg
     1921 ggcgcgagcg ctacagtcgc gccgcgcacc ccgtgtcacg ccggaggccc gatggcaacc
     1981 ttcgacagcc ccgatttcga cgatcacgag gcggtccatt ggttcaacga tacgcggtcg
     2041 ggcctgcgcg cgatcgtcgc catccacaag cgccgcggcg gccgggccgg cgggggtgcg
     2101 cgcatgatgg cctacccgga cgagcaggcg gcgctcaacg acgcgctgag gctgtcgcgg
     2161 gcgatgacct acaagcatgt cctgtgcggc agcccgttcg gcggcggcaa gatcatcgtc
     2221 ctcggcgacc cggcgcgcgc caagaccgac gccaagttcc gcgcgctcgg gcggatcatc
     2281 gactccttcg ccggcggcta cgtgaccggc gaggacgtcg gcaccacgcc ccgcgacatg
     2341 gcgctgatcc gcgagggcac gcgctgggtg atcggcgtcg agggcgccgg cggcgacacc
     2401 tcgccggcga ccgcctacgg cgtccagctc ggcatcgagg cggcggtgcg gcgcgccctc
     2461 ggcgcaacct cggtcgaggg cctcacgatc gcggtccagg gggtcggcaa cgtcggcttc
     2521 gcgctcgccc gccgcctcgc cgaggccggg gcgcggctgg tggcggccga cgccgacccg
     2581 gcgcgcgccc agcgcgccgc ggccgagctc ggcgcccgga tcgtggcccc ggagcagatc
     2641 ctggcggtcg aggccgaggt gctgagcccg tgcgcgctcg gcggcgtgct caacgacgcg
     2701 accatcccgc gcatccgcgc caaggtggtc gccggcgccg ccaacaacca gctcgccgag
     2761 gcccgccacg gcgccatgct ggccgagcgc ggcatcctgt acgcgcccga ctacgtgatc
     2821 aacgcgggcg gcgtgctgca tgcgctggtc gagctcgagg gtttcgatcc ggcgcgcgtg
     2881 ttccgcgacg tgaggcgcat ccgcgaggcg ctggaagagg tgttcgcgcg cgcggtgcgc
     2941 accggcgagc cgaccagtgc cgccgccgac gccatcgcgc gcgagcgcct ggcggccgag
     3001 gccgacggcg cgcaggccgc ataaaggagc ccgcgccccg aagggcgcgg gctgaaacgt
     3061 gccgggaccg gtgcgccgtc aggtgatgtg ggcgccgcgg gtctcgacgt agaccgcgta
     3121 cagcgactgg ctgccggtca tgaacagccg gttcttcttc gagccgccga agcagacgtt
     3181 cgagatgatc tcgggcatcc ggatctggcc gatgcggtca ccgttggggg cgaagatgtg
     3241 gaccccgtca tagccgtcgc cgacccagcc ggcgctcgac cagacgttgc cgtcctcgtc
     3301 ggcgcggatg ccgtcggcga agccggcgcg gccgtccagc tccatgtcgg cgaaggtgcg
     3361 cggattcttc agcgtcgcgc cgtcgacgtc gtagccccag atgatgttct tggcttcctt
     3421 gtagtgcgag gcgccggtgt cggcgatgta catcttcttg tagtcgttgg agaagcacag
     3481 gccgttgggc ttgaacggct cgtccgcgac cttggtcatc ttgccggtct tggcgtcgac
     3541 ccgataggtc gcctccttct ggatcggctg gaccgagccg gtgccgagcc tgtggccctc
     3601 gtagttcatc aggccgccat agccgggatc ggtgaaccag atcgagccgt cgtcgggatg
     3661 gaccacaacg tcgttgggcg cgttgagctc cttgccgtcg gcgctgtccg cgatcaccga
     3721 catcgagccg tcatgctcgt agcgcaccac ccggcggttg ccgtgctcgc aggcgatctg
     3781 gcggccctga tagtcgaagg tgttgccgtt cgagttgccg gacggatagc ggaacatctt
     3841 cgagacgtgc ccgtcttcct cgatccagcg cagctgcatg ttctccggga tgtcgctcca
     3901 gatcaggtaa cggccgacgc cgttccatgc gcagccttcg gcccacagcg tgccccgata
     3961 gagccgcagg atcggggtgt tgccgagctt gtacttgaag cgcgggtcga gccccatgac
     4021 gtcgggctcg ggatagcgca ccggctcgtc atcggggccg aagttgcgcg cgaatgcgct
     4081 cgacgacatg gcggtgatgc cggcgatcgc ggtcgccgcg gcaaggaacg accggcgatc
     4141 gattgcctgg tcggtcttgc gctcgaccgg cttcgccaag tcgatgaact tgtgctcttg
     4201 gactctacgc atgtgacttg cctccctcgt tgttttgcgc gagtaccatc ggcgcccgcg
     4261 cacagattgc tatgccgcgc gtcgcgcgcc ggccatgcgg aagccggcgg tgcgcccggc
     4321 gaagcccctc ccttcgagac cgctccgccg accggtcgag atggccgaga cgcggagccc
     4381 gggggatatt ggcgccgaat cgcggtcgcc gcaaccgtcg cgccggcggc cgtgccggct
     4441 gttcgacgct gatatgtctc acggcccgtc ctcgccctgc tcgcgggcat gcaggcgggc
     4501 aatgccggca accacctcgg cgacctcgga atcgggcagc ggcgggcggc atttgacccg
     4561 gttccagcac agcagcagct cgagcgcgac ctccggatcg acgccgtgcc acagcagatg
     4621 gccgaccagc gaggcggcag tgtcgttgcg ccgtccctcg gccacccccg cctcgaccag
     4681 cgagcgccaa taagcggtcg ggtggccgcg gcgcgcgccc tcccgcagca ccacgcgcag
     4741 cagccacgcc ggcatcggcg cgagcgcacg gcggtccggg tcgcgccccg cccgccaccg
     4801 gtagcgacgg cccgagggat ggatcgaggg cggcgccacc accaggccgc cgtcgccgcg
     4861 caggtcgatg cccggggcca gacccgcgcg attgtgcacc tcgccgcccg gatgcccgaa
     4921 atagaggtgg cggccgccgc cgccggtggt gcattcgacc gtctcgggca tcggaccgtg
     4981 ccgacgctcc caggcggcga tgctgtccgc cccgccatgc cccggatcga catcgaggac
     5041 caccagcctg gagag
//
LOCUS       DASWHU010000295         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2034425, whole genome shotgun sequence.
ACCESSION   DASWHU010000295 DASWHU010000000
VERSION     DASWHU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034425"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB88_10990"
     CDS             complement(<1..266)
                     /locus_tag="VGB88_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9648016.1"
                     /translation="MTLFARLAAITAGFVFVQIVLGAVVRLTNSGLSCPDWPLCYGLW
                     LPTPAKLAAIPGVDYSFGQVMAEWTHRLNAAAVVTPLVVVLVALA"
     gene            complement(289..1017)
                     /locus_tag="VGB88_10995"
     CDS             complement(289..1017)
                     /locus_tag="VGB88_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX9648017.1"
                     /translation="MPAASPDRAARRARNPAWLAWPAIALLVGLGSWQLQRAHWKEAL
                     IATMEARLAAPPIALPERLDAAAVEALGYRRILLRGRFLHSREMLVAPRTYQGRVGAE
                     LVTPFETEDGRVVLVDRGWVPDAAMAPARRPESRREGTVAFEGVVRPPPSPNPFTPAN
                     DPAHGAWYWIDPQAMARAAGIAPLPFTIAALAGEGGGGALPLARPARIELRNAHLYYA
                     LTWYALAVALGVVYLLFRRGARRA"
     gene            complement(1023..1400)
                     /locus_tag="VGB88_11000"
     CDS             complement(1023..1400)
                     /locus_tag="VGB88_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX9648018.1"
                     /translation="MVGRQLRGAISPLSAALRRRCPRCGEGRLFAGYLTVAERCEVCD
                     LDLRRQDTGDGPAVFVILLLGFIIVGLALAVEVRVAPPYWLHLILWLPLTIGLALVML
                     PPLKAWLIAQHYRHDLLDDRPGA"
     gene            complement(1382..2206)
                     /locus_tag="VGB88_11005"
     CDS             complement(1382..2206)
                     /locus_tag="VGB88_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9648019.1"
                     /translation="MTDAPAKHPYHLVAPSPWPVVGAAAALLLTVGMVMFMHEIAGGT
                     AVLAAGGVLVAATMVVWWRDVVREATFQGHHTPVVQLGLRYGMLLFIASEVMFFAAWF
                     WAFFDASLFADEAIQVARAEFTGGTWPPQGVETFEPFGVPFLNTVVLLTSGATVTWAH
                     HALREGNRGHLILGLWLTVGLGALFTSLQAYEYAHAPFGFTEGIYASTFFMATGFHGA
                     HVIIGTLFLIVCLGRAYAGHFKPEQHFGFEAAAWYWHFVDVVWLFLFTCVYWWGGS"
     gene            complement(2257..2850)
                     /locus_tag="VGB88_11010"
     CDS             complement(2257..2850)
                     /locus_tag="VGB88_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX9648020.1"
                     /translation="MTAARTRHLASAVLLGLVAGGMVGAAYAAVPLYALFCRVTGYGG
                     TTRIAAGPAPEVGERVITIRFNGDVQSGLPWRFTPVERQTTARVGETRLAYFRAENLG
                     DEPLVGSATFNVTPQIAGAYFDKIDCFCFTEQFLKPGEVAELPVSFYVDPAIVDDPDT
                     ANVRTITLSYMFFDAGADAVAVHLAAEARAPAAAKLN"
     gene            complement(2847..2969)
                     /locus_tag="VGB88_11015"
     CDS             complement(2847..2969)
                     /locus_tag="VGB88_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648021.1"
                     /translation="MPLHDEHRRRRLRNVALAVTLGALVLLFFLITIAKLSGNA"
     gene            complement(2989..>3880)
                     /locus_tag="VGB88_11020"
     CDS             complement(2989..>3880)
                     /locus_tag="VGB88_11020"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9648022.1"
                     /translation="RPGIGLRRTVAAYIALTKPRIIELLLITTVPAMVLAAKGLPPIA
                     LVFWTLVGGSLAAGSANAINCYLDRDIDSLMARTRRRPLPAHEVEPERAVLFGLVLGV
                     LSFVVLAFFVNLIAAFLAQLAIAFYVVVYTMLMKRSTPQNIVIGGAAGALPPVIGWAA
                     VTGSVSLAPLLLFAIIFMWTPPHFWALALVRSDDYARAGVPMLPVVAGGDETRRQIMI
                     YSIVLVPLAMLPAAIGVSGSVYALSAAALGIWFLHRARAVWRRRGDDDARRLFGASIA
                     YLFLLFAALVVDHWAQAAVG"
BASE COUNT          667 a         1373 c         1400 g          440 t
ORIGIN      
        1 gccagcgcga ccagcaccac gaccagcggc gtgaccaccg cggcggcatt gagccggtgg
       61 gtccactcgg ccatcacctg gccgaagctg taatcgacgc cgggaatcgc ggcgagcttg
      121 gccggggtcg gcagccacag gccgtagcac agcggccagt ccgggcagga caggcccgaa
      181 ttggtcagcc gcaccaccgc gccgagcacg atctgcacga acacgaaacc cgcggtgatc
      241 gcggcgagcc gcgcgaacag ggtcatccgc acctcatcct gcgcgccgtc acgcgcggcg
      301 cgcgccgcgc cggaatagca gatagacgac ccccagcgcg accgccaatg cgtaccaggt
      361 gagcgcgtag tagaggtgcg cgttgcgcag ctcgatgcgg gccgggcgcg cgagcggcag
      421 cgcgccgccg ccgccttcgc ccgccagcgc ggcgatggtg aacggcagcg gcgcaatgcc
      481 ggcggcgcgc gccatcgcct gcgggtcgat ccagtaccag gcgccgtgcg ccggatcgtt
      541 ggccggggtg aacgggttgg ggctgggtgg cgggcgcacc acgccctcga aagcgaccgt
      601 gccctcgcgt cggctctccg gacggcgcgc cggagccatc gccgcgtcgg gcacccagcc
      661 gcgatcgacc agcaccacgc gcccgtcctc ggtctcgaac ggcgtcacca gctcggcgcc
      721 gacgcggccc tgataggtgc gcggcgcgac cagcatctcg cggctgtgca ggaaccggcc
      781 gcggagcagg atgcgccggt agccgagcgc ctcgaccgcg gccgcatcca gcctctccgg
      841 cagtgcgatc ggcggcgccg cgagccgcgc ctccatggtc gcgatcagcg cctccttcca
      901 gtgcgcgcgc tggagctgcc agctgccgag gccgaccagc agcgcgattg ccggccacgc
      961 cagccaggcc gggttgcggg cgcggcgcgc ggcgcggtcc gggctcgccg cgggcaccgg
     1021 cgtcaggcgc ccggacggtc gtcgagcagg tcgtgacggt agtgctgggc gatcagccac
     1081 gccttgagcg gcggcagcat caccagcgcg aggccgatgg tcagcggcag ccacaggatc
     1141 aggtgcagcc agtagggcgg ggcgacgcgg acctcgaccg ccagcgccag cccgacgatg
     1201 ataaagccga gcagcaggat cacgaacacc gccgggccgt cacccgtgtc ctggcggcgg
     1261 aggtccaggt cgcacacctc gcaacgctcg gcgaccgtca gatagccggc gaacaatcgg
     1321 ccctcgccgc agcgcgggca gcgccgcctg agggccgcag acagcggtga gatcgcgccc
     1381 ctcagctgcc gccccaccag tagacgcagg tgaacaggaa cagccacacg acgtcgacga
     1441 aatgccagta ccaggcggcg gcctcgaagc cgaaatgctg ctccggcttg aagtggccgg
     1501 cataggcccg gcccaggcac acgatcagga acagggtgcc gatgatgacg tgggcgccgt
     1561 ggaagccggt ggccatgaag aaggtcgagg cgtagatgcc ctcggtgaag ccgaacggcg
     1621 cgtgcgcata ctcgtaggcc tgcagcgagg tgaacagcgc gccgagaccg accgtgagcc
     1681 acagcccgag gatcaggtgc ccgcggttgc cctcgcgcag cgcatgatgc gcccaggtca
     1741 cggtcgcgcc cgaggtcagc agcacgacgg tgttgaggaa cggcaccccg aacggctcga
     1801 acgtctccac cccctgcggc ggccaggtgc cgccggtgaa ctcggcacgc gcgacctgga
     1861 tcgcctcgtc cgcgaacagc gaggcatcga agaacgccca gaaccaggcg gcgaagaaca
     1921 tcacctcgga ggcgatgaac agcagcatgc cgtaacgcag cccgagctgg acgaccggcg
     1981 tgtgatggcc ctggaaggtt gcctcgcgaa ccacgtcgcg ccaccacacg accatggtcg
     2041 ccgcgaccag cacgccgccg gccgcgagca ccgccgtgcc gccggcgatc tcgtgcatga
     2101 acatgaccat gccgacggtg agcagcagcg ccgcggcggc gccgaccacc ggccacgggc
     2161 tcggggcgac caggtggtac gggtgcttgg cgggggcgtc ggtcatcact cggtctccgc
     2221 gatggtcacg tcggtgtgcg gcccgccggg cgcggctcag ttgagctttg cggcggccgg
     2281 ggctcgcgcc tcggcggcga gatggacggc gaccgcgtcg gcgccggcgt cgaagaacat
     2341 gtaggacaag gtgatggtac ggacgttggc ggtgtcggga tcgtcgacga tcgccgggtc
     2401 gacgtagaac gacaccggca gctcggcgac ctcgcccggc ttgaggaact gctcggtgaa
     2461 gcagaagcag tcgatcttgt cgaaataagc gccggcgatc tgcggcgtga cgttgaacgt
     2521 ggcgctgccg accagcggct catcgcccag gttctcggcg cggaagtagg cgagcctggt
     2581 ctcgccgacg cgcgccgtgg tctggcgctc gaccggggtg aagcgccacg gcaggccgga
     2641 ctggacgtcg ccgttgaagc ggatcgtgat cacgcgctcg ccgacctcgg gcgccgggcc
     2701 ggcggcgatg cgggtggtgc cgccatagcc ggtgacccgg cagaacaggg cgtacagcgg
     2761 caccgccgcg taggccgcgc cgaccatgcc cccggcaacc aggccgagca ggaccgccga
     2821 ggccaagtgc cgggtgcgtg ccgcagtcat gcgttgccgc tcagcttggc gatggtgatc
     2881 aggaagaaca gcagcaccag cgcgccgagc gtaaccgcca gcgccacgtt gcgcagccgc
     2941 cggcgcctgt gctcatcatg cagcggcatc accatcgtcc gccgcgccct agccgaccgc
     3001 ggcttgcgcc caatggtcga cgaccagggc ggcgaacagc aggaacagat aggcgatcga
     3061 ggcgccgaac agccgccgcg cgtcgtcgtc gccgcggcgg cgccacaccg cccgggcgcg
     3121 gtgcaggaac cagatgccga gcgcggcggc gctcaaggcg tagacgctgc cgctcacgcc
     3181 gatcgccgcc ggcagcatcg ccagcggcac cagcacgatg ctgtagatca tgatctggcg
     3241 ccgggtctcg tcgcctcccg ccaccaccgg cagcatcggc acgcccgcgc gcgcatagtc
     3301 gtccgagcgc accagcgcca gcgcccagaa atgcggcggc gtccacatga agatgatcgc
     3361 gaacagcaag agcggcgcaa gcgaaaccga gccggtcacc gccgcccagc cgatcaccgg
     3421 cggcagcgcg ccggcggcgc cgccgatcac gatgttctgg ggcgtcgagc gcttcatcaa
     3481 catcgtgtac acgaccacgt agaaggcgat cgccagctgc gccaggaacg cggcgatcag
     3541 gttcacgaag aacgccagca cgacgaacga gaggacgccc aggacgaggc cgaacaggac
     3601 ggcgcgctcc ggctcgacct cgtgcgcggg caacgggcgc cgccgcgtcc tcgccatgag
     3661 cgaatcgatg tcgcggtcga ggtagcagtt gatcgcgttc gcgctgccgg ccgcgagcga
     3721 cccgccgacc agcgtccaga acacgagcgc gatcggtggc aggcccttcg cggcgaggac
     3781 catcgcgggc acggtcgtga tcagcagcag ctcgatgatc cggggcttgg tcagcgcgat
     3841 gtaggccgcg accgtacgcc gcaggccgat gcccgggcgg
//
LOCUS       DASWHU010000296         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2035197, whole genome shotgun sequence.
ACCESSION   DASWHU010000296 DASWHU010000000
VERSION     DASWHU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035197"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..766
                     /locus_tag="VGB88_11025"
     CDS             29..766
                     /locus_tag="VGB88_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648023.1"
                     /translation="MIEFIELRKAFGAIQAVAGVSMIASDGRVTGLIGPNGAGKTTAF
                     RTVYGLLVPDAGSVRVDGIDVTADRIAVQRRLGVLPDVRGLYPRLTGREHIRFYGELQ
                     GLSGAALESRIDDLIERLGMGEFADRRARGYSRGQELKVALGRAMIHRPHNLILDEPT
                     NGLDVVSSRAVRQLICELRDRGHCILISSHIMAEVGALCDQLVIVSEGAVIAEGTPDE
                     LRRRFTASDLEDVFVASVSGAQMSGVS"
     gene            763..>1736
                     /locus_tag="VGB88_11030"
     CDS             763..>1736
                     /locus_tag="VGB88_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9648024.1"
                     /translation="MTPHGPVHRGPWPRLAVVVRKELRDHFRDKRSLLLALLYPLLGP
                     LLVAGSLYVAGDVLNRAQGSAGHISVPARGMEHALDFVRFLDAHDVGLYPAPRPPGDM
                     VRTGEVAVGLDIPPEAASGRFTVRVYVDFAKIGNLRASATIGDLISAYNRERAATLAQ
                     DAGLPEDFLVSVAIQQVNVSRPADMAVFLYNLMPPLVMFMIFLAGVHITVDMTAGERE
                     RGSLEPLLIAPVERAGLLFAKFAVGFLMTALAMVINLVGFRTLLGIVAGMHPAMTPPP
                     TLGAFLTIFAIAVPLMALAVAFQIAIALASRSMKEAQIYLGLLPLVPAL"
BASE COUNT          219 a          633 c          613 g          271 t
ORIGIN      
        1 gcccggcggg gggagggaca gggccggcat gatcgagttc atcgagctgc gcaaggcgtt
       61 tggcgcaatc caggcggtgg ccggggtgtc gatgatcgcc agcgacggcc gcgtgaccgg
      121 cctgatcggg cccaacggcg ccggcaagac caccgccttc cgcaccgtct acggcctgct
      181 ggtccccgat gccgggtcgg tgcgggtcga cggcatcgac gtcaccgccg accgcatcgc
      241 ggtgcagcgc cggctgggtg tgctgcccga cgtgcgcggg ctgtatccgc ggctcaccgg
      301 gcgcgagcac atccgcttct acggcgagct gcagggcctg tcgggggcgg cgctggagag
      361 ccgcatcgac gacctgatcg agcggctggg gatgggcgag ttcgccgacc ggcgcgcgcg
      421 cggctactcg cgcggccagg agctcaaggt cgcgctcggc cgcgccatga tccaccggcc
      481 gcacaacctg atcctggacg agccgaccaa cggcctcgac gtggtctcga gccgcgccgt
      541 gcgccagctg atctgcgagc tgcgcgaccg cggccactgc atcctgatct cgagccacat
      601 catggccgag gtgggggcgc tgtgcgacca gctggtgatc gtctccgagg gcgcggtcat
      661 cgccgagggc acgcccgacg agctgcgccg ccggttcacc gccagcgacc tcgaggacgt
      721 gttcgtcgcc tcggtcagcg gcgcccagat gagcggcgta tcgtgacccc gcacgggccc
      781 gtccaccgcg gcccctggcc gcggctcgcg gtggtcgtgc gcaaggagct gcgcgaccac
      841 ttccgcgaca agcgcagcct gctcttggcg ctgctctatc cgctgctcgg gccgctgctg
      901 gtcgccggca gcctgtacgt cgcgggcgac gtgctcaacc gcgcgcaagg cagcgccggg
      961 cacatctcgg tgccggcgcg cggcatggag cacgcgctcg acttcgtgcg cttcctcgac
     1021 gcccacgacg tcggcctcta cccggcgccg cgcccgcccg gcgacatggt gcgcaccggc
     1081 gaggtcgcgg tcgggctcga catcccgccc gaggccgcga gcggccggtt caccgtccgg
     1141 gtctatgtcg acttcgccaa gatcggcaat ctgcgcgcct cggccaccat cggcgacctg
     1201 atctccgcat acaaccgcga gcgcgcggcg accctcgccc aggacgccgg tctgcccgag
     1261 gacttcctgg tctcggtcgc gatccagcag gtcaacgtct cgcgcccggc cgacatggcg
     1321 gtgttcctgt acaacctgat gccgccgctg gtgatgttca tgatcttcct cgccggcgtg
     1381 cacatcacgg tggacatgac cgcgggcgag cgcgagcgcg gctcgctcga gccgctgctg
     1441 attgcgccgg tggagcgcgc cgggctcttg ttcgccaagt tcgcggtcgg gttcctgatg
     1501 accgcgctgg cgatggtcat caacctggtc ggcttccgca ccttgctcgg catcgtcgcc
     1561 ggcatgcacc cggcgatgac cccgccgccc acgctcggcg cgttcctgac catcttcgcg
     1621 atcgcggtgc cgctgatggc gctggcggtg gcgttccaga tcgccatcgc gctcgcctcg
     1681 cggtcgatga aggaggcgca gatctatctc gggctgctgc cgctggtgcc ggcgct
//
LOCUS       DASWHU010000297         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3481908, whole genome shotgun sequence.
ACCESSION   DASWHU010000297 DASWHU010000000
VERSION     DASWHU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481908"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGB88_11035"
     CDS             complement(<1..473)
                     /locus_tag="VGB88_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648025.1"
                     /translation="MVNRTLAALAAAAVVAAALPLAAQGAPYHIRGTLAGVDGSKLTI
                     ATDDGMSKTATLNDDARVFVVDRATIDDIKRGQFVGITSVYAEGEATALEVHIFEEAL
                     RGLAEGHYPWDLVEEENMMTNAGIAKLVEVGKDRKLTVTYVEGEPTTRKVGMQEIT"
     gene            complement(685..1014)
                     /locus_tag="VGB88_11040"
     CDS             complement(685..1014)
                     /locus_tag="VGB88_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648026.1"
                     /translation="MAGFGKKNLRLTELRAAMIHAAAQAYLKEVENKRLLTDEELKGV
                     VWRILTANGQQTNDREFDYATTGTKALMRERTYVQELASRWTRGTIGIGYDDMKRVDQ
                     ILAMQTR"
     gene            complement(1247..>1546)
                     /locus_tag="VGB88_11045"
     CDS             complement(1247..>1546)
                     /locus_tag="VGB88_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648027.1"
                     /translation="EARAVQSERYAGETRAGGAPVRTNSDADGTLLERVAAPDERGRA
                     LLTDAVDRLRLSARGYHRVLRVARTLADLDGAGGVARRHVAEALSYRRISPARAA"
BASE COUNT          191 a          564 c          538 g          253 t
ORIGIN      
        1 gtgatctcct gcatgccgac cttgcgcgtg gtcggctcgc cctcgacata ggtcaccgtc
       61 agcttgcggt ccttgccgac ctcgaccagc ttggcgatgc cggcgttggt catcatgttc
      121 tcttcctcga ccaggtccca cgggtagtgg ccctcggcga ggccgcgcag cgcctcctcg
      181 aagatgtgga cctcgagcgc ggtggcctcg ccctcggcgt agaccgaggt gatgccgacg
      241 aactggccgc gcttgatgtc gtcgatggtc gcgcggtcga ccacgaacac gcgcgcgtcg
      301 tcgttgaggg tcgccgtctt cgacatgccg tcgtcggtgg cgatggtgag cttggacccg
      361 tcgacacccg ccaaggtgcc gcggatgtgg tagggcgcgc cctgggccgc gagcggcagc
      421 gccgccgcga ccacggccgc cgcggcaagc gcggcgagag tgcgattgac catgtcttcc
      481 tccctcgatc ccggccgcag ggcctcgttg ggcccgcccc ggccgcggcc cctgaccagc
      541 ggggccgttc gctgttgccg ggcagcgggc gaggcaccgc ccatggcgcc cgccgcggcc
      601 cgcaggctgg tgtatcaccg cggccggcgt gcgtccatcg acgcgcaggc ggcggctggt
      661 cgcagcggcg cggcggccgc gcgcctagcg cgtctgcatg gccaggatct ggtcgacgcg
      721 cttcatgtcg tcgtagccga tgccgatggt gccgcgcgtc cagcggctcg ccagctcctg
      781 gacgtaggtc cgctcgcgca tcagcgcctt ggtgccggtg gtggcatagt cgaactcgcg
      841 gtcgttggtc tgctggccgt tggcggtcag gatccgccag acgacgccct tgagttcctc
      901 gtcggtcagc aggcgcttgt tctcgacttc cttgaggtag gcctgcgccg cggcatgaat
      961 catcgccgcc ctgagctcgg tcagcctgag attcttcttt ccaaacccgg ccatggcgag
     1021 gttccggcag ttccggcgcc cctgttgccc cgggggcgcg tgatccctga tagccgagcc
     1081 aacggcgcaa gccaaccggg tcaccgccgc cccgatcgtg cgagccgcgc ccggaccatc
     1141 tgcccgcgcc gccggacagg cttctaagtg cctactgtca aacggctttt ctcaattctc
     1201 tacaatttta gcaaaatcgg cgaccgggca caatcacgcg cccgcctcac gccgcacgcg
     1261 ccgggctgat gcggcggtag gagagcgcct cggcgacgtg gcggcgggcg accccgccgg
     1321 cgccgtcgag gtcggcgagg gtgcgcgcga cgcggagcac ccggtggtag ccgcgcgccg
     1381 acaacctgag ccggtcgact gcgtccgtga gcagcgcccg gccgcgctcg tccggcgccg
     1441 cgacgcgctc caagagcgtg ccgtcggcgt ccgaattggt gcgcaccggc gccccgcccg
     1501 cgcgcgtctc gccggcatag cgctcggact gcaccgcgcg cgcctc
//
LOCUS       DASWHU010000298         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2762833, whole genome shotgun sequence.
ACCESSION   DASWHU010000298 DASWHU010000000
VERSION     DASWHU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762833"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..794
                     /locus_tag="VGB88_11050"
     CDS             171..794
                     /locus_tag="VGB88_11050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9648028.1"
                     /translation="MRDRLARIHDRDIFLERAESEVRRAQRYGRTLTALMLAVDGRPA
                     IVKRYGRAWLELLMDIIGLICRENLRDPDILGRHNEHVLAIVLPEAGEASGVQLAARL
                     IAGLSAIRLPTANGELAFTVSAGVAATVGEVAELDHILRNAEDQLKAAQAAGGHQVKG
                     CFVGDRREHLDEALARLGRRRTDPDEVTQAVELTREELDVLLKRRVE"
     gene            999..1436
                     /gene="speD"
                     /locus_tag="VGB88_11055"
     CDS             999..1436
                     /gene="speD"
                     /locus_tag="VGB88_11055"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX9648029.1"
                     /translation="MDETTAVPTRTRGGGADTRIVADDHFVERDGCRFAGFHLIIDLW
                     QARRLDDLPHIKRTLRRAAEVAGATVLRLDAHRFESSDGISAVAILAESHISIHTWPE
                     LSYAAIDIFMCGDTRPHDAIPLLEAAFEAARVEVIEHRRGMLE"
     gene            1433..2281
                     /gene="speE"
                     /locus_tag="VGB88_11060"
     CDS             1433..2281
                     /gene="speE"
                     /locus_tag="VGB88_11060"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX9648030.1"
                     /translation="MIQFVERLFQEYTQLFSVERILYRERTEHQDLVIFENRIFGRVL
                     ALDGVIQTTERDNHVYHEMIAHVPILAHGSVREVLIIGGGDGGTLREVVKHDIARATM
                     VEIDAGVIELSRKYLPGLSDGAFDHARADVVIADGCAYVRETDRRFDVIIVDSTDPVG
                     PGAVLFTDEFYGACKRCLTDGGVLVTQSGVVLMQPDEVVTTHRRLGALFADASFFQAP
                     VPTYIGGPMTCGWASDDASLRRLPEAELAARYQRAGLHTRHYSPAIHLASFALPPELH
                     ELLARS"
     gene            complement(2301..3455)
                     /locus_tag="VGB88_11065"
     CDS             complement(2301..3455)
                     /locus_tag="VGB88_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648031.1"
                     /translation="MRPAGLIAAAVTLALEPGGAAVAADVPSPPDLALAADQVTTLAV
                     PFSFEVGPNAETRTVVLHYRLYLPRDYHDHPERAYPAMFMASPIGDATMGAFEQRLRA
                     ERWLVAMLIESRNQSQLWMPNFLAAHDDLVRRVRVQPGMLFCTGLSGAARACSAYPGL
                     REGFRGIILQGAGPWRGDVFAGEANRDLVVYGTFGAEDFNLPHALRIRRGLPPTVRRM
                     VEVWDGGHDWAPAPVAERALDWVEETAFVDADLGPAASDAAWWYATVLADRFEQSGPG
                     IARYVVAREAQAMLALHGALLDAESESRLAQVADMAAVLAAEPGLVREVEAWEAYRTA
                     LSFEEAGDGHTLPEAEARYAAVAERLGDTEYGRLAALRAQSIGREVALLR"
BASE COUNT          551 a         1324 c         1178 g          530 t
ORIGIN      
        1 aacccgctcc cgatcgccgg cgcgcgcggt cagttaacca ttccctcatt tgtcggccga
       61 aaaataagga tgcgatcgag cgatggcgat tgcgagttcg cctcgagcgc ccgatagttc
      121 atcccctcgg tggccgcgct cgaacgccac catagcccaa caattctgcg atgcgcgacc
      181 gcttggccag aatccacgac cgggacatct tcctggagcg cgccgaatcc gaggtccgcc
      241 gcgcccagcg ctatggccgc acgctcaccg cgctgatgct ggcggtcgac ggccggcccg
      301 ccatcgtcaa gcgttacggc cgtgcctggc tcgagctgct gatggacatc atcggcctga
      361 tctgccgcga gaacctgcgc gaccccgaca tattggggcg ccacaacgag cacgtgcttg
      421 ccatcgtcct gcccgaggcc ggcgaggcct cgggcgtcca gctcgccgcc cggctgatcg
      481 ccgggctgtc ggcgatccgc ctgccgacgg cgaacggcga gctcgccttc accgtcagcg
      541 ccggcgtcgc ggcaaccgtg ggcgaggtgg cggagctcga ccacatcctc aggaatgccg
      601 aggaccagct caaggccgcc caggcggcgg gcggccacca ggtcaagggc tgctttgtcg
      661 gcgaccggcg cgagcacctg gacgaggccc tcgcacggct cgggcgcagg cgtacggacc
      721 ccgatgaggt cacgcaggcg gtcgagctga cccgggaaga gctcgacgtg ctgctcaagc
      781 gccgcgtcga gtgagcgcgc ccgcgccgga gcgctgaccg ggccggcccc ggtcctcggc
      841 acgaatttgc gcttgcggcg caaaaatctc ttgccttccg cgccgaaatg tcgctttttg
      901 cgcgccgtcg gcgtgccccg gacgcgtcgg acaacacttc ggctcgtttg cagaaggggg
      961 tggccatggg cgcgcaccac gccccatccc gaatgggctt ggacgaaacg accgcggtcc
     1021 cgacccgtac gcgcggcggg ggcgccgata cgcgcatcgt cgccgacgat catttcgtcg
     1081 agcgcgacgg ctgccggttc gccgggttcc atctgatcat cgacctgtgg caggcgcgcc
     1141 gactggacga cctgccccat atcaagcgta ccctgaggcg ggccgcggaa gtcgccggcg
     1201 cgaccgtgct caggctcgat gcgcaccgct tcgagtcgag cgacggcatc tccgcggtgg
     1261 cgatcctggc ggaaagccac atcagcattc acacctggcc cgagctatcc tacgcggcga
     1321 tcgacatatt catgtgcggc gacacccgcc cccacgatgc cattcccctg ctcgaggccg
     1381 cgttcgaagc ggcccgggtc gaggtcatcg agcatcgccg gggcatgctt gaatgatcca
     1441 gttcgtcgag cgcctgtttc aggagtacac gcagctcttc tcggtcgaac ggatcctcta
     1501 ccgcgagcgg accgagcatc aggacctggt catcttcgag aatcgcatct tcggccgcgt
     1561 gctggccctc gacggtgtca tccagacgac cgagcgcgac aaccacgtct atcacgagat
     1621 gattgcgcac gtgccgatcc tcgcccacgg gtcggtgcgc gaggtgctga tcatcggcgg
     1681 cggcgacggc ggcaccttgc gcgaggtggt caagcacgac atcgcccgcg cgaccatggt
     1741 cgagatcgac gccggcgtca tcgagctgtc gcgcaagtac cttcccggcc tgtccgacgg
     1801 cgcgttcgac catgcgcgcg ccgacgtcgt catcgccgac ggctgcgcct atgtgcgcga
     1861 gacggatcgc cggttcgacg tcatcatcgt tgattcaacc gatccggtgg gcccgggcgc
     1921 cgtgctgttc accgacgagt tctacggcgc gtgcaagcgc tgcctcaccg acggcggcgt
     1981 gctggtgacc cagagcggcg tggtgctgat gcagccggac gaggttgtaa ccacgcaccg
     2041 ccggctcggc gccctgttcg ccgacgcgag cttttttcag gcgccggttc cgacctatat
     2101 cggcggcccg atgacctgcg gctgggcgag cgacgatgcg tccttgcgcc gcctgccgga
     2161 ggccgagctc gccgcgcgct accagcgtgc cggcctgcat acccgccact actctccggc
     2221 gatccacctc gcgagcttcg cgctgccgcc cgagctccac gagctgctcg cccgatcctg
     2281 accgccgtcg caccgcgctc ctatcgcaac agcgccactt cgcggccgat cgactgcgcg
     2341 cgcagcgccg ccagccggcc gtactcggtg tcgccgaggc gctccgcgac ggcggcgtag
     2401 cgtgcctcag cctccggcag ggtgtggccg tcgccggctt cctcgaacga cagcgccgtg
     2461 cgatacgcct cccatgcctc gacctcgcgt acgagcccgg gctcggcggc aagcacggcc
     2521 gccatatcgg caacctgcgc cagccgcgac tcgctctcgg catcgagcag cgcgccgtga
     2581 agcgccagca tcgcctgtgc ctcgcgcgcc acgacgtagc gggcgatccc cggccccgac
     2641 tgctcgaacc ggtcggcgag cacggtcgcg taccaccacg ccgcgtccga cgccgccgga
     2701 ccgaggtcgg catcgacgaa cgccgtctcc tcgacccagt cgagcgcccg ctccgcgacc
     2761 ggcgccggcg cccagtcatg gccgccgtcc cacacctcga ccatccttcg caccgtcggc
     2821 ggcaggccgc gccggatgcg cagcgcatgc ggcaggttga aatcctcggc cccgaaggtg
     2881 ccgtagacca ccaggtcgcg gttggcctcg ccggcgaaca cgtcgccccg ccacggcccg
     2941 gccccttgca ggatgatgcc gcgaaagccc tcgcgcaggc cgggataggc gctgcaggcg
     3001 cgtgcggcgc ccgacaaccc ggtgcagaac agcatgcccg gctggacgcg cacgcggcgc
     3061 accaggtcgt cgtgggcggc aaggaagttc ggcatccaca gctgcgactg gttgcgcgat
     3121 tcgatcagca tcgccaccag ccagcgctcg gcgcgcagcc tctgctcgaa cgcgcccatt
     3181 gtcgcatcgc cgatcggcga ggccatgaac atcgccgggt aggcgcgctc ggggtgatcg
     3241 tgataatcgc gcggcaggta caggcgatag tgaagcacga ccgtccgcgt ctccgcgttc
     3301 gggccgactt cgaaggagaa cgggaccgca agcgtggtca cttggtccgc agctagcgca
     3361 agatcgggcg ggctcggtac gtcggctgcc accgccgcgc cgccgggttc cagcgccagc
     3421 gtcactgctg ccgcgatcag tccggccggc cgcatcacgc gttcacggtc cgctcgacca
     3481 gccagaacag gccgagcgct ccgatcgtca gcgacgccgc atgcaccacg accgcgcgcc
     3541 gcgcctcggg gccgcgaatc cggtcgatgg ccagcagcac cgg
//
LOCUS       DASWHU010000299         5825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1276628, whole genome shotgun sequence.
ACCESSION   DASWHU010000299 DASWHU010000000
VERSION     DASWHU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276628"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGB88_11070"
     CDS             <1..384
                     /locus_tag="VGB88_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648032.1"
                     /translation="GSAVNLVVSSGPAPGTMHVGDLDGTGSAAVWWWWTAQATITVHD
                     GNHAPVAGVLVSGTGSAGGASASASCTTNSAGQCTVSSTVWIWVASASFTVNGLSGGG
                     LTYESTANHDPDGSSNGTTVSVNRL"
     gene            465..1814
                     /locus_tag="VGB88_11075"
     CDS             465..1814
                     /locus_tag="VGB88_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX9648033.1"
                     /translation="MSLYAALAKRARDGRPVRVGLIGAGKFGSMFLAQVPTMTGVHVL
                     AIADLSVERARAALNRVGWDAARAAAAGIDRALRDGTTWVCDDPMAVIAAAPVEVVID
                     ATGDPLAGVRHALAAIDHGKHVVMVNVEADALVGPALARRAAEAGVVYSLAYGDQPAL
                     ICELVDWARACGFAVAAAGKGTKYLPAFHASTPETVWANFGIDPDEAARDRLNAQMFN
                     SFIDGTKSAIEMCAVANATGLTPAPGGLTFPPCGTEELATVLRPSSHGGSLHHAGQVE
                     VVSSLQRDGSRVERDLRHGVYVVIEAPNDYTARCLRQYGLAIDPSGRFAAMYKPYHLI
                     GLELAVSVASAALRGEPTGAPAAFRADAVAVAKRALAPGEVLDGEGGYSVWGRLSPAA
                     DSIAAGALPIGLAHGARIERAVAKGAVVTLKDVTLAGADQALALRRELAPAHPARAA"
     gene            complement(1837..2046)
                     /locus_tag="VGB88_11080"
     CDS             complement(1837..2046)
                     /locus_tag="VGB88_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX9648034.1"
                     /translation="MNGGKAVIRNNFFRILRRIGESRDGATAIEYGLICALIALVIVG
                     SVTMVGTEMTTTYDSIASAVSSVSN"
     gene            complement(2159..3805)
                     /locus_tag="VGB88_11085"
     CDS             complement(2159..3805)
                     /locus_tag="VGB88_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9648035.1"
                     /translation="MAAGSVAIGHVISVGGAQVTAALSKFHAQFGDAAAEAADAAIDG
                     DSAVRIGSLVKMRTPASTIYGIVTKLATDVEPGPAGPVERNIADIDLMGEILRGPKAN
                     GGPSFERGVSIMPTLGVPLMATDTSDVALVYAQPSASNVRIGTVHQDKTTPAYLMIDD
                     LLAKHIAVLGTTGSGKSSAVALILRKILSGHPAGHVILLDPHNEYRNAFLDLAEVIQP
                     ENLSLPCWLLNMDEMAAVLVRGGDPADREAQLANLRDAIVEAKRRFAGRDADAHALTV
                     DTPVPYRLTELIRIIDQSMGKLDKPDTSAPYLRLLARLEQINSDKRYAFMFSGLLVRD
                     TMPAVMSRMLRIPVRGKPLTIVDLSGVPSEIVDVVVSVVARMVFDFALWAERDRRPPI
                     MLVCEEAHRYIPEQDLGESFLSTKRALSRIAKEGRKYGVSLCLVTQRPSELSSTVVSQ
                     CGTLFALRMNNHKDQRFVEAAMPENGLGLLAALPALRRQEAVVVGEGVSVPMRLRFDT
                     LPPEHLPRSGDAPFAEAWSKETVDPSFVLDSITRWRRQQR"
     gene            4021..4998
                     /locus_tag="VGB88_11090"
     CDS             4021..4998
                     /locus_tag="VGB88_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648036.1"
                     /translation="MSALIEIDSVSKRFGALTAVDGVSFKVARGELLGFLGPNGAGKT
                     TTMRMITGFLEPDGGNIRVSGHDVVADQVAAKALIGYLPEGAPLYGDMTPSGLLRFVT
                     AARGLPAKRAAERIDWAVARLGLGSVLAQSIDTLSKGFKRRLGLAQALVHDPEVLILD
                     EPTDGLDPNQKHEVRRLIREMAPDKAIIISTHLLDEVDAVCSRALIINRGRIVADGTP
                     VELAARSSYHNAVTVTVQGVAAGDVERSLRAIAGVAKVSVATVDGGVRCTLFPAGART
                     IVADVSRQLQAVGWQIDQFGVETGRLEEVFRTVTTGAQGAAKGAKAAAR"
     gene            4995..5729
                     /locus_tag="VGB88_11095"
     CDS             4995..5729
                     /locus_tag="VGB88_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9648037.1"
                     /translation="MSAVWAIFKRELAGYFATPVAYVFIVIFLFLTGIFTFYLGDFYE
                     RGQADLQPFFAWHPWLYLFLVPALGMRLWAEERRTGTIELMMTLPVSVTAAVLGKFLA
                     AWAFTAISLALTFPVWITVNYLGQPDNGVIVAGYLASLLMAGGFLAISACISALTKNQ
                     VIAFVVAVVACFLFTVSGLPLVLDAFSAWAPQALVQTIASFSFLTNFADMMRGVIDLR
                     HIVYFGSLIAVWLFANVVAVEHIKAA"
BASE COUNT          850 a         1994 c         2066 g          915 t
ORIGIN      
        1 ggcagcgcag tcaacctggt ggtgtcgagc ggcccggcgc cgggcacgat gcatgtcggc
       61 gatttggacg gcacgggctc ggccgcggtt tggtggtggt ggactgccca ggcgaccatc
      121 accgtccacg acggcaacca cgccccggtc gcaggcgtgc tggtgagcgg caccggcagt
      181 gccggcggcg ccagtgcatc ggcctcgtgc acgaccaatt cggcggggca atgcacggtc
      241 agctcgacgg tctggatctg ggtggcgagc gcgagcttca cggtcaacgg gctgtcgggc
      301 ggcggcctga cctacgagtc gaccgccaat cacgaccctg acggcagcag caacggcacc
      361 acggtctccg tcaaccggct ttgacggcgt cgctcgctta aggatgtcgc catcctcgcc
      421 gatggcgcta gactgcgccg agtaggttag ctgggagagg cacgatgtcg ctttacgcag
      481 cgctcgccaa gcgcgcccgg gacggccgcc cggtccgcgt cgggctgatc ggcgccggca
      541 agttcggcag catgttcctg gcgcaggtgc cgaccatgac cggcgttcat gtgctcgcga
      601 tcgccgatct gtcggtcgag cgcgcgcggg cggcgctgaa ccgggtcggc tgggacgcgg
      661 cgcgcgccgc cgccgccggt atcgaccggg cgctgcgcga cggcaccaca tgggtctgcg
      721 acgacccgat ggcggtgatc gcggcggcgc cggtcgaggt ggtgatcgac gccaccggcg
      781 atccgctggc cggggtgcgc cacgcgctcg ccgccatcga ccacggcaag cacgtggtca
      841 tggtcaacgt cgaggccgac gcgttggtcg gcccagcctt ggcgcgccgc gccgccgagg
      901 ccggggtggt ctactcgctc gcctatggcg accagccggc gctgatctgc gagctggtcg
      961 actgggcgcg cgcctgcggc ttcgcggtcg ccgccgccgg caagggcacc aagtacctgc
     1021 cggccttcca cgcctccacg ccggagacgg tgtgggcgaa tttcggcatc gacccggacg
     1081 aggccgcgcg cgaccggctg aacgcgcaga tgttcaactc gttcatcgac ggcaccaagt
     1141 cggcgatcga gatgtgcgcg gtggccaacg ccaccgggct gaccccggcg ccgggcgggc
     1201 tcaccttccc gccttgcggc accgaggagc tggcgacggt gctgcgcccg tccagccacg
     1261 gcggcagcct gcatcacgcc ggccaggtcg aggtggtgtc ctcgctgcag cgcgacggca
     1321 gtcgtgttga gcgcgacctg cgccacggcg tctacgtcgt catcgaggca cccaacgact
     1381 acaccgcccg ctgcctgcgc caatacggcc tggcgatcga cccctccggg cgtttcgccg
     1441 cgatgtacaa gccctaccac ctgatcggtc tcgagctcgc ggtgtcggtg gcgagcgcgg
     1501 cattgcgcgg cgagccgacc ggcgcgccgg ccgcgttccg cgccgacgcg gtcgcggtcg
     1561 ccaagcgcgc actggcgccg ggcgaggtgc tcgacggcga gggtggctac agcgtgtggg
     1621 ggcggctgtc gcccgccgcc gacagcatcg ccgccggcgc gctgccgatc gggctcgccc
     1681 acggcgcgcg catcgagcgg gcggtggcga aaggcgcggt ggtgacgctg aaggacgtga
     1741 cgcttgccgg cgcggaccag gcgctcgcgc tcaggcgcga gcttgcgccc gcgcacccgg
     1801 cgcgggcggc ataggcgcgg ccgcggggcg ggccgcctag ttagagacgc tgctgacggc
     1861 gctggcgatg ctgtcgtagg tggtcgtcat ctcggtgccg accatcgtga cgctgccgac
     1921 gatgaccagc gcgatgagcg cacagatcaa gccgtactcg atcgcggtgg cgccgtcgcg
     1981 cgattcgcca atgcgtctta atattctaaa aaaattgttt cgtatcacgg ccttgccgcc
     2041 attcattaag aatcgctcca gagtcgatat agggcgcggg aaagccctag caatggtttc
     2101 gcaggcggat tgaaaccggg cgaggccgcg gccgtgagcg gggccgggcg gggggcggtc
     2161 agcgctgctg gcgccgccag cgggtgatcg agtcgagcac gaacgacggg tccacggtct
     2221 ccttggacca ggcctcggca aacggcgcgt cgccgctgcg cggcaggtgc tcgggcggca
     2281 gggtatcgaa ccgcaaccgc atcggcacgc tcacgccctc tccgaccacc accgcctcct
     2341 ggcggcggag cgccggcagc gcggcaagca ggccgaggcc gttctcgggc atcgcggcct
     2401 cgacgaacct ctggtccttg tgattgttca tgcgcagcgc gaacagcgtc ccgcactgcg
     2461 acacgacggt cgaggacagc tcggacggcc gctgggtcac caggcacagc gagacgccgt
     2521 acttccgccc ctccttggcg atccgcgaca gcgcccgctt ggtcgacagg aagctctcgc
     2581 cgaggtcctg ctcggggatg tagcggtgcg cctcctcgca caccagcatg atcggcggcc
     2641 ggcggtcgcg ctccgcccac agggcgaaat cgaacaccat gcgcgcgacc accgagacta
     2701 cgacgtcgac gatctcggac gggacccctg acaggtcgac gatggtcagc ggcttgcccc
     2761 ggaccgggat ccgcagcatc cgcgacatca ccgccggcat ggtgtcgcgc accagcaggc
     2821 ccgagaacat gaacgcgtag cgcttgtccg agttgatctg ctcgagccgg gccagcaggc
     2881 gcaggtaggg cgccgaggtg tcgggcttgt cgagcttgcc catgctctgg tcgatgatcc
     2941 ggatcagctc ggtcagccga tacggcaccg gggtgtcgac ggtcagcgca tgggcgtccg
     3001 cgtcgcggcc ggcgaagcgg cgcttggcct cgacgatggc gtccctgagg ttggcaagct
     3061 gggcctcgcg atcggccgga tcgccgccgc ggaccagcac cgccgccatc tcgtccatgt
     3121 tcaagagcca gcacggcagc gacaggttct cgggctggat cacctcagcg aggtcgagaa
     3181 aggcgttgcg gtactcgttg tgcgggtcga gcaggatcac gtggccggcc gggtggccgc
     3241 tcaggatctt gcgcaggatc agggcgaccg cgctcgactt gcccgagccg gtggtcccca
     3301 gcacggcgat gtgcttggcc agcaggtcgt cgatcatcag ataggcgggc gtcgtcttgt
     3361 cctggtggac ggtgccgatc cgcacgttgc tggccgaagg ctgggcgtag acgagcgcca
     3421 cgtccgaggt gtccgtggcc atgagcggca cgccgagcgt cggcatgatc gacacgccgc
     3481 gctcgaagct cggcccgccg ttggccttgg gcccgcgcag gatctcgccc atcaggtcga
     3541 tgtcggcgat gttgcgctcg accgggccgg ccggtcccgg ctcgacgtcg gtagcgagct
     3601 tggtcacgat gccgtagatg gtcgaggccg gggtgcgcat cttgaccagc gacccgatgc
     3661 gcacggccga atcgccgtcg atggcggcgt cggcggcttc ggccgcggcg tcgccgaact
     3721 gggcatggaa cttggacagc gccgcggtca cctgcgcgcc gccgaccgag atcacatgcc
     3781 cgatcgcgac cgatcctgct gccatgccat gaatcccggc tgatgcccgt ccaacaactt
     3841 gcgatcttat caaaatacgt ccgacgcaac cgcatttcga gtgccgtgcg taccttccgg
     3901 cgacaaatcc ccttgaaagc gccgggccgg gcttctacct atgcgccggg ttttgcctgg
     3961 taaaagtcgc gcccaatcag agggatggcc ctccgagacc ggccgggagg gggagagcgg
     4021 gtgtcggcat tgatcgagat cgacagcgtt tccaagcgtt tcggcgcgct gaccgcggtc
     4081 gacggggtgt cgttcaaggt cgcgcgcggc gagctgttgg gcttcctcgg ccccaacggc
     4141 gccggcaaga ccaccaccat gcgcatgatc accggcttcc tcgagcccga cggcggcaac
     4201 atccgggtca gcggccacga cgtcgtcgcg gaccaggtcg cggccaaggc gctgatcggc
     4261 tacctgcccg agggcgcgcc gctgtacggc gacatgaccc cgagcgggct gttgcgcttc
     4321 gttaccgccg cgcgcgggct gccggccaag cgcgccgccg agcgcatcga ctgggcggtc
     4381 gcgcggctgg ggctcggctc ggtgctggcg cagtcgatcg acaccctgtc gaaaggcttc
     4441 aagcggcggc tcgggctggc ccaggcgctg gtccacgacc ccgaggtgct gatcctcgac
     4501 gagccgaccg acggcctcga ccccaaccag aagcacgagg tgcgccgcct gatccgcgag
     4561 atggcaccgg acaaggcgat catcatctcg acccatctcc tggacgaggt cgacgcggtg
     4621 tgcagccgcg ccctgatcat caaccgcggc cgcatcgtcg ccgacggcac cccggtcgag
     4681 ctggccgcgc ggtcgagcta ccacaacgcg gtcacggtca cggtccaggg cgtcgccgcg
     4741 ggcgacgtcg agcgctcgct gcgggcgatc gcgggcgtcg ccaaggtctc ggtcgcaacc
     4801 gtcgacggcg gggtgcgctg cacgctgttc cccgccggcg cgcgcacgat cgtcgccgac
     4861 gtcagccggc agctgcaggc ggtcggctgg cagatcgacc agttcggggt cgagaccgga
     4921 cggctggagg aggtgttccg gaccgtcacc accggcgccc agggggccgc caagggcgcg
     4981 aaagcggcgg cccgatgagc gccgtctggg cgatcttcaa gcgcgagctg gcgggctact
     5041 tcgccacccc ggtggcctat gtgttcatcg tcatcttcct gttcctgacc ggcatcttca
     5101 cgttctacct gggcgacttc tacgagcgcg ggcaggccga cctgcagccg ttcttcgcct
     5161 ggcacccgtg gctgtacctg ttcctggtgc cggcgctcgg catgcgcctg tgggcggagg
     5221 aacggcgcac cggcaccatc gagctgatga tgaccctgcc ggtgtcggtg accgcggcgg
     5281 tgctcggcaa gttcctcgcc gcctgggcgt tcaccgcaat ttcgctggcg ctcaccttcc
     5341 cggtctggat caccgtcaac tatctgggcc agcccgacaa cggcgtgatc gtcgccggct
     5401 atctggcgag cctgctcatg gccggcgggt tcctggcgat ctcggcgtgc atctcggcgc
     5461 tgaccaagaa ccaggtgatc gcgttcgtgg tcgcggtggt ggcgtgcttc ctgttcacgg
     5521 tcagcggcct gccgctggtg ctcgacgcgt tcagcgcgtg ggcgccgcag gcgctggtcc
     5581 agaccatcgc ctcgttcagc ttcctgacca acttcgccga catgatgcgg ggcgtgatcg
     5641 acctcaggca catcgtctat ttcgggtcgc tgatcgcggt ctggctgttc gccaacgtgg
     5701 tcgcggtcga gcacatcaag gcggcctaga gcgcgatgat attaagtcgg atccctgagc
     5761 ctgttttggt cgtcaccccg gactccgcga agcggatatc cggggtccat acgcctttgc
     5821 cacgc
//
LOCUS       DASWHU010000300         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_909919, whole genome shotgun sequence.
ACCESSION   DASWHU010000300 DASWHU010000000
VERSION     DASWHU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909919"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGB88_11100"
     CDS             <1..300
                     /locus_tag="VGB88_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648038.1"
                     /translation="VESWTWAFSDYTGASGRAWGYGTTTFDNGDKWFTAFSGTAQAQP
                     TTDGESRRTFTGTGTITGGTGVYKGVRGTIRLDSRFDPATGYNLAETAVEYWIEQ"
     gene            complement(372..448)
                     /locus_tag="VGB88_11105"
     tRNA            complement(372..448)
                     /locus_tag="VGB88_11105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Pro,seq:cgg)
     gene            complement(510..1532)
                     /locus_tag="VGB88_11110"
     CDS             complement(510..1532)
                     /locus_tag="VGB88_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9648039.1"
                     /translation="MALAVGLESAGVEPNRDALRRLHALVGDDLAAVNRVIVERMHSP
                     VALIPQLAGHLTASGGKRLRPLVTLLAARMCGYGGERHIPLAACIEFIHTATLLHDDV
                     VDASALRRGSATANAVWGNQASVLVGDFLLSRAFEMMVEDGALEVLSILARASAVIAE
                     GEVTQMMTSNDTSTTQSVYLEVITAKTARLFAAAAEIGAVVADRPAAERQALFEYGLN
                     LGISFQLIDDGLDYGAAQVTLGKSIGDDFREGKVTLPVLLAYARGTADDREFWRRTIE
                     QGRQREGDLSHAIDLLNRSEAIADTVARARAYGERARSALGGFAAGDYRDALVETIDF
                     AIERAY"
     gene            1707..1925
                     /locus_tag="VGB88_11115"
     CDS             1707..1925
                     /locus_tag="VGB88_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX9648040.1"
                     /translation="MRELLRTNDLVKISWVRSVLADAGIEAVVLDAHASVVEGSVLAI
                     QRRIMVLDDDLAEARRVLDDVGESYPRD"
     gene            1897..2685
                     /locus_tag="VGB88_11120"
     CDS             1897..2685
                     /locus_tag="VGB88_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9648041.1"
                     /translation="MLARAIRATDGAGDAGIGEDRLLGGRVRLRQPRRGYRAAIDPVL
                     LAAAVPAAPGELVADLGTGAGAAALCLLARCPGCRVTGFELQPGLAALAADNARLNGL
                     EDRFSVVAGDIARAAQDWRGRFDRVMANPPYLPAARADMSAGDDPATVEGGAGLGDWV
                     RAALALVRPKGTVALIHRADRLDELLAHLAAGAGGIVVMPLWPKPGLAAKRVVVCARR
                     GVRAPLTLGPGLVLHDARGCYTGAAEQVLRAAQGLFEAQSHGGV"
     gene            2736..>2957
                     /locus_tag="VGB88_11125"
     CDS             2736..>2957
                     /locus_tag="VGB88_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX9648042.1"
                     /translation="MDLGFLPFATRAPVVAVMRLAGVVSPAPQPFRGALNLAGMAGLI
                     ERACTLRGVRALALQVNSPGGSAVQSTLIA"
BASE COUNT          396 a         1051 c         1078 g          432 t
ORIGIN      
        1 gtggagagct ggacctgggc gttctcggac tataccggcg ccagcggccg cgcctggggc
       61 tacgggacga ccaccttcga caacggcgac aagtggttca cggcattttc cggcaccgcg
      121 caagcgcagc cgacgacgga cggcgagagc cggcgtacct tcaccggcac cggcacgatc
      181 accggcggca ccggcgtcta caagggcgtc aggggcacca tacgcctgga tagccgcttc
      241 gaccccgcga ccggctacaa cctcgccgag accgcagtcg agtactggat cgagcaatag
      301 ccgggccgag gcgacggttc acttgcacca tcgcacgctg tcctgcggca ccgttaaggt
      361 cttgaaatcg ttggtcggag tgagtggatt cgaaccaccg gcccccgcct cccgaaggcg
      421 gtgctctacc aggctgagct acactccgac cgggcccgga ccggcgccta tatagcggct
      481 ggccggctcc ggggcaagac ggcggcggat cagtaggcgc gctcgatcgc gaagtcgatc
      541 gtctcgacca gggcatcgcg gtagtcgccg gcggcgaacc ccccgagcgc gctccgcgcc
      601 cgctcgccat aggcacgcgc gcgcgcgacc gtgtcggcga tcgcctcgct gcggttaagc
      661 agatcgatag cgtgcgaaag gtcgccctcg cgctggcgcc cctgttcgat cgtccgccgc
      721 cagaactccc ggtcgtctgc cgtgccgcgg gcgtaggcga gcagcaccgg cagggtgacc
      781 ttgccctcgc ggaagtcgtc gccgatgctc ttgccgagag tgacctgggc ggcgccgtaa
      841 tcgaggccgt cgtcgatcag ctggaaactg atgccgagat tgaggccgta ctcgaacagc
      901 gcctggcgct ccgcggcggg ccggtcggcg accaccgcgc cgatctcggc ggcggcggcg
      961 aacaggcgcg cggtcttggc ggtgatcacc tccagataga ccgactgcgt ggtgctggtg
     1021 tcgttcgagg tcatcatctg cgtcacctcg ccctcggcga tcaccgcgct cgctctcgcc
     1081 aggatcgaga gcacctcgag cgcgccgtcc tcgaccatca tctcgaacgc ccggctgagc
     1141 aggaaatcgc cgaccagcac gctcgcctgg ttgccccaca ccgcgttcgc ggtcgccgag
     1201 ccgcggcgca gcgcgctggc gtcgaccacg tcgtcgtgga ggagcgtcgc ggtgtggatg
     1261 aactcgatgc aggcggcaag cgggatgtgg cgctcgcccc catagccgca catgcgggcg
     1321 gccagcaagg tcaccagcgg gcgcaaccgc ttgccgcccg aggcggtcag atggccggcc
     1381 agctgcggga tcagcgccac cgggctgtgc atccgctcga cgatcacccg gttgacggcg
     1441 gcaaggtcgt ccccgaccag cgcatgcagc cggcgcaagg cgtcgcggtt cggctcaacg
     1501 cctgcgctct cgagtccgac ggctagcgcc acttcaccga tccccttgca ggcccggctc
     1561 ggcgtcaacc cgccgatccc ggccgatgac cttgacgcct gccgcggcgc ggcggagcat
     1621 agaagcggcc tcggcccgcg gtcaagcgca tggccgggcc gcccggccgc gcaagtgcga
     1681 gcctattatg taagtggcga tgcccggtga gggaactact ccgtaccaac gatctggtca
     1741 aaatctcgtg ggtccgctcg gtgctcgccg acgcgggcat cgaggcggtg gtgctcgatg
     1801 cccatgcgag cgtggtcgag ggctcggtcc ttgccatcca gcgccggatc atggtcctcg
     1861 atgacgacct cgccgaagcg cgccgcgtcc tcgacgatgt tggcgagagc tatccgcgcg
     1921 actgatggcg ccggcgacgc cggcatcggc gaggaccgcc tgctcggcgg gcgtgtgcgc
     1981 ctgcgccagc cccggcgcgg ctatcgggcg gcgatcgatc cggtgctcct cgccgccgcg
     2041 gttccggccg cccccggcga gctggtggcc gacctcggca ccggcgccgg cgcggcggcg
     2101 ctgtgcctgc tcgcgcgctg tcccgggtgc cgggtcaccg gcttcgagct gcagccgggc
     2161 ctggccgcgc ttgccgccga caacgcccgc ctcaacggcc tcgaggaccg gttctcggtg
     2221 gtggccggcg acatcgcccg cgcagcgcag gattggcgcg gccggttcga ccgggtgatg
     2281 gcgaacccgc cctatctgcc cgccgcgcgc gccgacatgt ccgccggcga cgatccggcg
     2341 accgtcgagg gcggggcggg gctcggcgac tgggtccggg cggcgctggc gctggtgcgc
     2401 ccgaagggga cggtggcgtt gatccaccgt gccgaccgcc tggacgagct gctggcgcat
     2461 ctcgccgccg gcgccggcgg catcgtcgtg atgccgctgt ggccgaagcc ggggctcgcc
     2521 gccaagcggg tggtggtgtg cgcccggcgc ggcgtgcgcg cgccgctcac gcttggcccc
     2581 gggctggtgt tgcacgatgc ccgggggtgc tataccggag cggccgagca ggtgctgcgc
     2641 gcggcacagg ggctgttcga ggcgcaatcg cacggcggcg tttgacggcg ggccggatgc
     2701 ggacttgagc ccggccagga agcgggacta cgccgatgga cctcggtttt ctgcccttcg
     2761 cgacgcgcgc gccggtggtg gcggtgatgc ggctcgccgg ggtcgtctcg cccgcgccgc
     2821 agccgtttcg cggggcgctg aacctggctg gcatggcggg gctgatcgag cgcgcctgca
     2881 ccttgcgcgg ggtgcgcgcg ctggcgctgc aggtcaactc gccgggcggc tcggcggtcc
     2941 agtcgaccct gatcgcc
//
LOCUS       DASWHU010000301         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2765228, whole genome shotgun sequence.
ACCESSION   DASWHU010000301 DASWHU010000000
VERSION     DASWHU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765228"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VGB88_11130"
     CDS             complement(<1..1177)
                     /locus_tag="VGB88_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX9648043.1"
                     /translation="MDWELAAVAAKAATDETKDGKPARTLCLVDGSGFIFRAFHALPP
                     LTRADGTPVNAVLGFCNMLYKLARDYRCDAIAVLFDTARVTFRNDIYPDYKANRPEPP
                     AELVPQFPLVREATRAFGLPAIEVAGFEADDLIATYVRDARARGDRVTIVSSDKDLMQ
                     LVGDGVVMLDPIKMREIGADEVRERFGVGPDKVIDVQALAGDSTDNVPGVPGIGVKTA
                     AQLIEAFGDLDTLLARAGEITQPKRRQTLLDNAELARTSRELVTLRDDAPVPEPVASF
                     GPLDPEPEALTAFLKAQEFKALLARIQADLGWQAEAGPAPGRAPQPKAKDIAYELVQS
                     VDELQRWADAAVEAGRVAVDTETTSLDAMRAKLVGVSLSIEPGRACYVPLGHRPPDGE
                     "
     gene            1344..>1608
                     /locus_tag="VGB88_11135"
     CDS             1344..>1608
                     /locus_tag="VGB88_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9648044.1"
                     /translation="MNETGLEAQGAAGPRLIAGRLPVVPGSGPGVAWRLAAFYGTAFL
                     LIGLYMPFWPVWLQSRGLDAGEIGVVLALTTWTKVVASPLFAGL"
BASE COUNT          202 a          569 c          601 g          236 t
ORIGIN      
        1 gctcgccgtc gggcgggcgg tggccgagcg gcacgtagca cgcgcgcccc ggctcgatcg
       61 acagcgacac gccgacgagc ttcgcgcgca tggcatcgag cgaggtggtc tcggtgtcga
      121 ccgcgacccg gccggcctcg acggcggcgt cggcccagcg ctgaagctcg tccacgctct
      181 gcaccagctc gtaggcgatg tccttggcct tgggctgcgg cgcccgtccc ggcgccggcc
      241 ccgcctcggc ctgccagccg aggtcggcct ggatgcgcgc gagcagcgcc ttgaactcct
      301 gcgccttgag gaacgcggtc agcgcctcgg gctcggggtc gagcgggccg aagctcgcca
      361 ccggctccgg caccggcgcg tcgtcgcgca aggtcaccag ctcgcggctg gtgcgcgcga
      421 gctcggcgtt atcaagcaag gtctggcggc gcttgggctg ggtgatctcg ccggcgcgcg
      481 cgagcagcgt gtcgaggtcg ccgaacgcct cgatcagctg cgccgcggtc ttgaccccga
      541 tgccgggcac gccgggcacg ttgtcggtcg agtccccggc cagcgcctgg acgtcgatca
      601 ctttgtcggg gccgaccccg aagcgctcgc gcacctcgtc cgcgccgatc tcgcgcatct
      661 tgatcgggtc gagcatgacc acgccgtcgc cgaccagctg catcaggtcc ttgtcggagg
      721 agacgatggt cacccggtcg ccgcgggcgc gcgcgtcgcg cacataggtc gcgatcaggt
      781 cgtcggcctc gaaccccgcg acctcgatcg ccggcaggcc gaaggcgcgg gtcgcctcgc
      841 gcaccagcgg gaactgcggc accagctccg cgggcggctc gggccggttg gccttgtagt
      901 cggggtagat gtcgttgcgg aaggtgacgc gcgcggtgtc gaacagcacc gcgatggcgt
      961 cgcaccggta gtcgcgcgcg agcttgtaga gcatgttgca gaagccgagc acggcattga
     1021 ccggggtgcc gtcggcgcgg gtgagcggcg gcagcgcgtg gaacgcgcgg aagatgaaac
     1081 ccgagccgtc gaccaggcac aaggtgcgcg ccggcttgcc gtccttggtc tcgtccgtcg
     1141 ccgccttggc cgccaccgcc gcgagctccc agtccatccc cgagcttgac ccggggacca
     1201 ccggcgacca tgcccgagga cgcgccgcaa gtcgagttgc gcttgacccg cgcgccgctc
     1261 tggccgatcc agccttcgta gccgaagcgc ctgcgtcggc ggagtaggct tgccgccgct
     1321 caggccaagg ggtgggcgcg cgcatgaacg agaccggact cgaggcgcaa ggtgcggcgg
     1381 gaccgcggct gatcgccggc cggttgccgg tggtccccgg gtcaggcccc ggggtggcct
     1441 ggcggctggc cgcgttctac ggcaccgcct tcctgctgat cggcctgtac atgccgttct
     1501 ggccggtgtg gctgcaaagc cgggggctcg atgccggcga gatcggcgtg gtgctggcgc
     1561 tgaccacctg gaccaaggtg gtggcgagcc cgctgttcgc cggcctcg
//
LOCUS       DASWHU010000302        10914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2766100, whole genome shotgun sequence.
ACCESSION   DASWHU010000302 DASWHU010000000
VERSION     DASWHU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766100"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VGB88_11140"
     CDS             <1..134
                     /locus_tag="VGB88_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648045.1"
                     /translation="YGGDVTRKRKLLEKQKRGKKRMRQVGNVEIPQEAFLAALKLET"
     gene            complement(812..1963)
                     /locus_tag="VGB88_11145"
     CDS             complement(812..1963)
                     /locus_tag="VGB88_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9648046.1"
                     /translation="MAAQRLGVILNGITGRIGVNQHLRWAIGPIIAEGGVGLADGSRI
                     ELDPVLVGRDEDKLAAVARRFDIARWTTDLDAALGDPADTVFFDATTTDRRVANVRKA
                     LAAGKHVYVEKPTATNAEEAVALYRAARDAGVRHGVVQNYLWLPGFLKLRRLIESGFF
                     GRVLAIRIDFGYWIFEGDWQRGQRPSWNYRKEDGGGIVLDLMPHWRYVLETLFGAVES
                     VSCLALNQIAERRDEQGRRYRATADDAAFATLLLAGGVVVQVSNSWCTRIRRDDLSVF
                     QVDGTHGSAVVGLYDCLIQPRAATPAPVFNPDPANRPHFFADWRPVPDNQAFESAFKV
                     QWQLFVRHVVEGAPFPHDLRAGAKGVQLAELAYRSSAERRWIDVPEIPE"
     gene            complement(1977..3002)
                     /locus_tag="VGB88_11150"
     CDS             complement(1977..3002)
                     /locus_tag="VGB88_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9648047.1"
                     /translation="MTRRLAIIGLGMGVAPHAKSLCDLAGRVEVVWAASRTAERCRAF
                     AERFQFPTTTDVEAAITDPRVDAVILVTPPSTHLDLVRRAAGAGKHVLLEKPVEIDTA
                     RSTAVVEAAAGAGITLGVVLQFRFRDPSRRLRALVDDGSLGDIACAGVVVPWWRPQSY
                     YDQPGRGTRARDGGGVLITQAIHTLDLFQSLVGGVREVAAVAATTHAHRMECEDFVAA
                     GIVLANGAPGALRATTAAYPGFAERIELFGTRASAILDGATLEVSHRNGRTESFGDGQ
                     GTGGGADPMDFPHDAHRAVLVDFLDALDDGRAPAVSGAEALKVHRLIDALLESAQTGR
                     RAAVAAG"
     gene            3158..3820
                     /locus_tag="VGB88_11155"
     CDS             3158..3820
                     /locus_tag="VGB88_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9648048.1"
                     /translation="MPVGDSRRGAGGSLPRRARNPEATRRRILRAATGEFARLGLSGA
                     RVDRIAERARVNKRMIYHYFGSKDELFLAVLEGAYANIRNAERRLDLEHLAPEEAIRT
                     LVTFTWNYYLDNPGFLRLVNSENLHKARHLKRSKVIKDMHSPFVEMVADILRRGARHG
                     IFRKGVDPVQLNMTIAALGYYYLTNRYTSSIIFDRDLMAPAALDARLAFIIDNVLGYL
                     RP"
     gene            complement(3890..4231)
                     /locus_tag="VGB88_11160"
     CDS             complement(3890..4231)
                     /locus_tag="VGB88_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648049.1"
                     /translation="MKKSEIIKPFSWGVATGAIVLTIVAFSAGWVVTSGARTVQVRTA
                     WIDGQASICASLAQAHRQESGDVTDLSGYQARVARDDLAKAFAVALVGEAQADPRVVN
                     ACSDLLNKRDT"
     gene            5002..6501
                     /locus_tag="VGB88_11165"
     CDS             5002..6501
                     /locus_tag="VGB88_11165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9648050.1"
                     /translation="MFWPRERGRFGGQSSCRDNWHNLPRPSGSRGPVQGAARQRVAAG
                     DPRRCGRRVKTSSLTQFSDLGLARPILDALSAERYTRPTPIQTQAIPHVLAGRDLFGI
                     AATGTGKTAAFALPILDRLAATRRTGSRPRGSCRVLVLSPTRELAAQIAERFRAYGRK
                     LGVRVAVVVGGLPFKPQASALAGGVDVLVATPGRLLDHLGQGTARLDLTEVLVLDEAD
                     HMLDLGFIPAVRELIGLLPPRRQSLLFSATMPAPIKALAADFLRDPETVSVSPPDTPA
                     ERIDQSLIHVGQGRKGDLLAEILRAHDRGRVLVFTRTKRGADRVVRGLAASRLAAAAI
                     HGNKNQNQRTLALAGFRAGHTPILVATDIAARGIDVHDIALVVNFDLPNVPETYIHRI
                     GRTARAGAAGVAVSFCGPDEVPQLRAIEALIRARIPARSDARSSATKPAGPTTPHRDR
                     ATPAGAAGGTMADLPFMRTTKDRSRGSRRRRRDRPARSRPETQAMLSTA"
     gene            complement(6848..7558)
                     /locus_tag="VGB88_11170"
     CDS             complement(6848..7558)
                     /locus_tag="VGB88_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9648051.1"
                     /translation="MTRDGGLRREPSGARPGHRLSSATTFPGERAADRFVHVLGVAAS
                     LAAAGALMIVAIANLETLPVLSLAVYATGLAAGFGLSAGYHLVTREALRKTLRRLDHA
                     AIFVLIAGTYTPFALVSIGGPWGHALLAAVWAIALVGIAAKVLLPGRFERAAIVLYLV
                     QGWAGLAVLNPLVNGVSATTLSLLGAGGVLYTLGVVFHLWRGLRYQTAIWHAFVLVAA
                     ACHYVAVLDAVALAGADR"
     gene            complement(7741..7944)
                     /locus_tag="VGB88_11175"
     CDS             complement(7741..7944)
                     /locus_tag="VGB88_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9648052.1"
                     /translation="MAQGKVKWFNPGKGYGFIEPDDGSGDVFVHKTAVERAGLATLAE
                     GQTVSFDVASDKGKTHAVNLQAN"
     gene            8362..9498
                     /locus_tag="VGB88_11180"
     CDS             8362..9498
                     /locus_tag="VGB88_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9648053.1"
                     /translation="MLYETDDHRQIRDTARRFADQVIRPQAADLDRDELFPAEIYARM
                     AELGLFGITAPAADGGAGMDTLAYAVVMEELARGYASIADQCGLVELIATLLTRHGTA
                     AQKARHLGPILRAETRVAYCITEAEAGSDVAGVRTTAERARDGWELSGSKLWIHNAPV
                     ADLAFVLARTDKEAGRRGMSIFIVDCAAPGVTRGPKEHKMGQRASQVGALGFDSVALG
                     PDALLGEEGRGFHIMMSALDKGRVGIAALAVGIAQAALEAALAYVPQRRQFGRPIADF
                     QGIQWLLADMAKDVAAARLLTHQAAVTLDAGGRATLECSMAKCFASDAAVLHTANAVQ
                     IYGGSGYIRGFEVERLYRDAKITQIYEGTNQIQRTIIARQLLGG"
     gene            9503..10294
                     /locus_tag="VGB88_11185"
     CDS             9503..10294
                     /locus_tag="VGB88_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HEX9648054.1"
                     /translation="MCADSTRPVALVTGARRGIGRATALALARAGFDVAANDLEAGDG
                     LAATVRAVESEGARACAVPGDIAAIDGHAALLDAVTSGLGRLDCLVNNAGVSVLSRGD
                     LLDVSPESYDRCLAVNTRGTFFLTQAFARRLVAAPPPDDGIPRSIVTITSVNAEAASI
                     NRGEYCISKAALSMASRLFALRLAEVGAGIGVWEVRPGFIETEMSAPSKARYDDYISR
                     GMTAIRRWGRPEDVAQVIVTLASGGLPYAVGEAIHVDGGLTIAKF"
     gene            10342..10743
                     /locus_tag="VGB88_11190"
     CDS             10342..10743
                     /locus_tag="VGB88_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648055.1"
                     /translation="MHGSRPLALAYALAILVLGPGAFGADATYAFRGYSTTLIRGQAL
                     FGEMTEACRHDFGGKARLCSAGEWSASAAAAAPRAAAWIGPDAGDGIGNDQCRLAPAR
                     GLGLALFPDGRLVGISCDRFRPVTCCTPRVR"
BASE COUNT         1701 a         3856 c         3759 g         1598 t
ORIGIN      
        1 gctacggcgg cgacgtcacc cgcaagcgca agctgctgga gaagcagaag cgcggcaaga
       61 agcggatgcg ccaggtcggc aacgtcgaga tcccgcagga agcgttcctc gccgcgctca
      121 agctggagac atagcgtcca ttcatcgcga ccccgggctt gaccccaggg cgattgcata
      181 tggcgaaggt cccgggcggt gagccgccct taggcacgcg gcccgtcctc cgcagcagcg
      241 gcagggccgc tgccgctgcg gagggtggaa ggcgcggagc acgcggagaa ggcggaaagg
      301 aaaaccacag agtgcaatga ggccacagaa gtaggttttc tctgtgtcct ctgtgtcctc
      361 tgtggttgat cccgaccacg ccccttccgt gtcctccgcg cctccgcgtg aggaaaatga
      421 cagccggcta tccgacactg cccgaacaag caatcgacgc acatgcgatc gccatggccc
      481 gcgggggagg gcttttggtc ttgaacgtcc cccgcttgcg ggctatcgga ttcacacaat
      541 ccaccgcctg ggatggcgat gaagagctgt tgaaattgaa agaaaatgtt cccacgccgc
      601 cattgcgagg agaccggcga atgccgggcg acgaagcaat ccagcccgtc gtcggccgcg
      661 cacgccggcc tggattgccg cgcgccctcc cgccttcgcg aagcccgctt cggcgggcga
      721 agcaaggtcg ggcgctcgca acgacggtcg aaatagacgt gtgaatacca tagcgcttgc
      781 ggggggaggt aggtgggggt gcctcggcgc attattccgg gatctcgggc acgtcgatcc
      841 aacgccgctc ggcggagctg cggtaagcga gctcggcgag ctggacgccc ttcgcgcccg
      901 cgcgcaggtc gtggggaaac ggcgcaccct cgaccacatg gcgcacgaac agttgccact
      961 gcaccttgaa cgcgctctcg aacgcctggt tgtcgggcac cgggcgccag tcggcgaaga
     1021 agtgcgggcg gttggccggg tctgggttga acaccggtgc cggcgtcgcg gcgcgcggct
     1081 ggatcaggca gtcgtacagg ccgaccaccg cgctgccgtg ggtgccgtcg acttggaaca
     1141 ccgacaggtc atcgcggcgg atgcgggtgc accacgagtt cgacacctgg accaccaccc
     1201 cgccggcgag caggagggtc gcgaacgcgg cgtcgtcggc ggtggcacga tagcgccggc
     1261 cctgctcgtc ccggcgctcg gcaatctggt tgagcgcgag gcagctcacg ctctcgaccg
     1321 cgccgaacag cgtctccagc acgtagcgcc aatgcggcat caggtcgagc acgatgccgc
     1381 cgccgtcctc cttgcggtag ttccacgacg gccgctggcc gcgctgccag tcgccctcga
     1441 agatccagta gccgaagtcg atgcggatcg cgagcacgcg gccgaagaag cccgactcga
     1501 tcagccgcct gagcttgagg aaccccggca gccacaggta gttctggacc acgccgtgcc
     1561 gcacgcctgc gtcgcgcgcg gcacggtaga gcgccaccgc ctcctcggcg ttggtcgcgg
     1621 tcggcttctc gacgtagacg tgcttgccgg cggcgagcgc cttgcggacg ttggcgaccc
     1681 gccggtcggt cgtggtggcg tcgaagaaca cagtgtccgc cgggtcgccc agcgcggcgt
     1741 cgaggtcggt ggtccagcgt gcgatgtcga accggcgcgc gactgcggcg agcttgtcct
     1801 cgtcgcgccc gaccagcacc gggtcgagct cgatgcgcga accgtccgcg agcccgaccc
     1861 cgccctcggc gatgatcggg ccgatcgccc aacgcaggtg ctgattgacc ccgatgcgcc
     1921 cggtgatgcc gttgagaatc acgccgagcc gttgtgcggc catcgcccgc gcctcctcag
     1981 cctgccgcca ccgcggccct gcgcccggtc tgcgccgact cgagcagggc gtcgatcaag
     2041 cggtgcacct tgagcgcctc ggcgcccgag accgccggcg cgcgcccgtc atcgagcgcg
     2101 tcgaggaagt cgaccagcac cgcccggtgg gcgtcgtgcg ggaagtccat cgggtcggcg
     2161 ccgccgccgg ttccctggcc gtcgccgaag ctctcggtgc ggccattgcg gtggctgacc
     2221 tcgagggttg cgccgtcgag gatcgcgctg gcgcgcgtgc cgaacagctc gatccgctcg
     2281 gcgaagccgg gataggcggc ggtggtcgcg cgcagcgcgc ccggggcgcc gttggcgagc
     2341 acgatgccgg cggcgacgaa gtcctcgcac tccatccggt gggcgtgcgt cgtcgccgcc
     2401 accgcggcga cctcgcgcac cccgccgacc aggctctgga acaaatcgag ggtgtggatc
     2461 gcctgggtga tcagcacccc gccgccgtcg cgcgcgcggg tgccgcgccc gggttggtca
     2521 tagtaggact gcggccgcca ccacggcacc acgacgccgg cgcaggcgat gtcgccaagg
     2581 ctgccgtcat cgaccagcgc ccgcagcctc cggctgggat cgcgaaaccg gaactgcagc
     2641 accacgccga gagtgatccc cgcgccggcc gcggcctcga ccacggccgt gctgcgcgcg
     2701 gtgtcgatct cgaccggctt ctcgagcagc acgtgcttgc ccgcgcccgc ggcgcggcgc
     2761 accaggtcga gatgggtgct cggcggggtg accaggatta ccgcgtcgac ccgcgggtcg
     2821 gtgatcgccg cctcgacgtc ggtggtggtc gggaattgga agcgttcggc gaacgcgcgg
     2881 cagcgctccg ccgtgcggct cgccgcccac accacctcca cgcgcccggc gaggtcgcac
     2941 aggctcttgg catgcggcgc gacccccatg ccgagcccga tgatggcgag cctgcgagtc
     3001 atccccctcc ccggtggccg gcggccggcc ttgatgcggg ccaatcatcg gccggccggt
     3061 tgcgcggcgc aaggcgcggc ggcgataatg ggcggcgagt gccgcgcgcg cacccgaagg
     3121 tggcgtgcgc gacggcctcg attggggggg cagccagatg cccgttgggg acagtcgccg
     3181 cggcgcgggc ggctcgctgc cgcgccgggc gcgcaatcct gaggcaaccc gccggcgcat
     3241 cctcagggcg gcgacgggcg agttcgcgcg cttagggctg agcggcgccc gcgtcgaccg
     3301 catcgccgag cgggcgcggg tcaacaagcg catgatctac cactacttcg gctccaagga
     3361 cgagctgttc ctggcggtgc tcgaaggggc ctacgccaac atccgcaacg ccgagcggcg
     3421 cctcgacctc gagcatctgg cgcccgaaga ggcgatccgc accctggtca ccttcacctg
     3481 gaactactac ctcgacaacc cgggcttcct gcgcctggtc aacagcgaga acctgcacaa
     3541 ggcccgccat ctcaagcggt cgaaggtgat caaggacatg cactcgccgt tcgtcgagat
     3601 ggtcgccgac atcctgcgcc ggggcgcgcg ccacggcatc ttccgcaagg gcgtcgatcc
     3661 ggtccagctc aacatgacca tcgccgcgct cggctactac tacctgacca accgctacac
     3721 ctcgtcgatc atcttcgacc gcgacctgat ggcgccggcg gcgctcgacg cccgccttgc
     3781 cttcatcatc gacaacgtgc tcggctatct gcgcccgtga gcgcaccgcg gtcgcgcacg
     3841 ccgcaggttt cgaccggatc ccgatcggtt ggcggcgccg atcggaaggt cacgtgtcgc
     3901 gcttgttgag caggtccgaa caggcgttga cgacgcgggg atcggcctgc gcctcgccca
     3961 ccagggcgac ggcaaaggcc ttggccaggt catcgcgcgc cacgcgggcc tggtagccgg
     4021 acaggtccgt gacatcgccc gattcctggc ggtgggcttg agccagcgag gcgcagatcg
     4081 acgcctgccc gtcgatccag gcggtgcgta cctggacggt cctcgcgccg ctggtgacca
     4141 cccagccggc gctgaaggca acgatggtca agacgatggc gccggttgcg acgccccacg
     4201 agaacggctt gattatttcc gacttcttca tgacgaactc cttgtacgtg actgatgtgg
     4261 actgcacgaa ctgctttgga ctcatgcggt tccagccggc cgtcgagcca accggagcgc
     4321 gcggcgcgga tcaaccgccc ggccgaagcc ggtccaaacc ctcgtcagtg gacggcgtag
     4381 gccatgcttg cgattgcgat caggctgacg acggccgcga gcagggtcca ctggtagatc
     4441 gtcaggctcg gcgaaatgtt catgatcact cccttgttca ggtcgggcac gccgcggccc
     4501 atcgtcatcg gccgcagcat tgagccggga cctgcgatgt gcgcaccgac gcgtcgcgtt
     4561 gtggccgcgg cttgcgagtt gatctcgacc cgcgccgccg gccgtggctc cgacgaaatg
     4621 ggaagggtga gatcctgctt ggccgctgtc ggccggccgg atcgccgcgc taagtaccat
     4681 accgcctgat cgcgattgca gcaagcaaaa gttgctcggg atttcgcgga gttcggaaat
     4741 cattaggtga aacccaacat tatcagcgag ttaatattta tctcttctgt cgttttattt
     4801 tagtcggctg gcatgggagt tgccggcccg accggacccg tcttgcgccg cctgccaggc
     4861 gcagggccgc agggcgcggc cggagcggcg acacgagcat ggcgaaccgc gagtggctcg
     4921 ggccaggcgg tgcttcgatg tattcggttg actttccggc cgccgtaccc acgtatccgg
     4981 tagtcgtatc gacgacagct ggtgttctgg ccgcgtgaac gaggccgctt cggcggccaa
     5041 tcgtcctgcc gcgataactg gcataatctt cctcgaccgt ccggatcacg cgggccggtc
     5101 caaggggccg cacgacagcg cgtcgccgca ggtgacccgc gtcgatgcgg acgacgtgta
     5161 aagacatcat cattgaccca attctccgac ctcggcctcg cgcggccgat cctcgacgcg
     5221 ctgtcggccg agcgatacac ccggccaact cccattcaaa cccaggccat tccgcacgtg
     5281 cttgcgggac gtgacctgtt cggcatcgcc gcgaccggca ccggcaagac ggcggcgttc
     5341 gccctgccga tcctcgatcg cctggcggcc acgcgccgga ccggatcgag gccgcgcggc
     5401 tcctgccgcg tgctcgtgct cagtccgacg cgcgagctcg ccgcgcagat cgccgagcgc
     5461 tttcgcgcct acgggcgcaa gctcggggtt cgcgtcgcgg tcgtcgtcgg cggcttgccc
     5521 ttcaagccgc aggccagcgc gctggcgggc ggcgtggacg tgctcgtcgc gacgcccggg
     5581 cgtttgctcg atcacctggg ccagggcacg gcgcggctcg acctgaccga ggtcctggtc
     5641 ctggacgagg ccgaccacat gctcgacctg ggcttcatcc cggcggtccg cgagctgatc
     5701 gggctgcttc cgccaagacg gcagagcctg ttgttctcgg ccaccatgcc ggccccgatc
     5761 aaggcgctcg ccgccgactt cctgcgcgat cccgaaaccg tatcggtgtc gccacctgac
     5821 acgcccgcag agcgcatcga ccagagcctg atccacgtcg gccagggccg gaaaggcgat
     5881 ctgcttgccg agattcttcg cgcccacgac cgcggccggg tgctcgtgtt cacccgcacc
     5941 aagcgcggcg ccgaccgggt cgtgcgcggc ctggccgcgt cacggcttgc cgccgcggcg
     6001 atccacggca acaagaacca gaaccagcgg acgcttgcgc tggccggctt ccgtgccggc
     6061 cataccccga tactggtcgc caccgacatc gcggcgcgcg gcatcgacgt tcacgacatc
     6121 gcgctggtgg tgaacttcga tctgccgaac gtgccggaaa cctacatcca ccgcatcggg
     6181 cgcacggccc gcgccggcgc cgccggtgtc gccgtctcgt tctgcgggcc ggacgaagtc
     6241 ccgcagctcc gtgccatcga ggcgctgatc cgcgcgcgca ttcccgcccg atcggacgcg
     6301 cgctcgagcg cgacgaagcc ggcggggccc actacgccgc accgggaccg ggcgacccct
     6361 gcgggggccg ccggcgggac catggctgac ctgccgttca tgcgcacgac caaggaccgg
     6421 agccgcgggt cgcgtcgccg tcgccgcgat cgcccggcaa ggtcgcgacc cgaaacgcag
     6481 gctatgctgt cgaccgcgta gcggccaccc gggcaccttg gggctcggcg gcgtggatcg
     6541 gcgcgtcgcc gcgccgcccg gtgccactgg aggaaacgag ccatggccaa gagaccgccg
     6601 caacaaggcg ccgggtttcg gcgctacaag gagcctggct tgccgcgcgt gtccggcgac
     6661 gcgccgcgag aacggcggtg catgacctgc ggggcggact ttctcagcga gggctggcac
     6721 aaccgactct gccggcgctg tcgcacgcgc agccagccgg cgggcaacta tgggccgaac
     6781 ggcgccccgc tctcgcggcg gagcacgtcg aattgacacc gcctgcccac tcccgattgc
     6841 gaccggctca tcggtcggcc ccggcgagcg ccacggcatc gagaacggcc acataatggc
     6901 aagcggcggc aaccagaacg aaggcatgcc agatcgcggt ctggtatcgc agccctcgcc
     6961 acagatggaa caccacgccc agcgtgtaga ggacaccgcc ggcgcccagc agtgacagcg
     7021 tggtcgccga tactccgttg accaacgggt tcaaaaccgc cagacctgcc cacccctgca
     7081 ccaggtagag cacgatcgct gcccgctcga aacggccggg cagcaggacc ttggccgcga
     7141 taccgaccag cgcgatcgcc cacacggcgg cgagcaaagc atgcccccat gggccgccga
     7201 tgctgaccag ggcgaacggc gtgtaggtgc cggcgatcag gacgaagatc gcggcatggt
     7261 cgagccggcg cagggttttc ctgagggcct cccgagtgac gaggtggtag ccggccgaca
     7321 gtccgaaccc ggcagccaaa ccggtcgcgt agaccgcgag gctgaggacc ggcagggtct
     7381 ccaggttggc gatggcgacg atcatcaacg cgcccgccgc cgccaggctc gccgcgacgc
     7441 cgaggacatg gacgaagcgg tctgccgcgc gttcgccggg gaacgtcgtc gcactcgaca
     7501 atcggtgtcc ggggcgcgcg cccgacggtt cgcgtcgcag gccgccgtct cgggtcatgg
     7561 gcgcagtctt aaggccggcg ccgggccccg cgccagcgga ctttggccgg cgaccggccg
     7621 acgggctgca ggcaaaaata aaggacggcg gccctccctt acccgacgtc ccaggtgtct
     7681 gttcttcagt tcggagggcg gcggcttagg accgcacgtc ttatcccccg atccggacga
     7741 tcagttagct tgaaggttga ccgcgtgggt cttaccctta tccgaggcga cgtcgaaact
     7801 gaccgtctga ccctcggcga gtgtggccag accggcgcgc tcgactgccg tcttgtgcac
     7861 gaacacatcg cccgaaccgt cgtccggttc gatgaacccg tatcccttgc cggggttgaa
     7921 ccacttcacc ttaccctgag ccatatcggc ctcccaaagg gtgacgtgca cgcgcccgtc
     7981 gcgtgaaccg acgcaatcgt cgtttgcgga gatatcctgg acgaacgccc gaaggccgaa
     8041 cgacaagacc gccggaagcg cagattggct gttgctacat ggcccccggc cggccgaggt
     8101 acaaggcgga gggatcaaga ttcgtccgat gccggcgcgg caggcgatca ccgcaacctt
     8161 gatttctggg cggtcggccg ccgcgttcgc cccggcgcgg gcccggtcaa ccctgacggc
     8221 gggacaaccg aggcccgacg acatcgcccg cagcgtcccg gttatgccat catcgacgct
     8281 gccactgcgc cccgcttgac aggcgcgccg ggcaggacct aggaagtaac cgattggtta
     8341 ctatacggac ggcgtggaga gatgctctac gagaccgacg accaccgcca gatcagggac
     8401 acggcgcggc gcttcgccga ccaggtgatc cgcccgcagg ccgccgacct cgaccgcgac
     8461 gagcttttcc cggccgagat ttatgcgcgc atggccgagc tcggcctgtt cggcatcacc
     8521 gcgccggccg cggacggcgg cgccggcatg gacaccctgg cttacgcggt ggtcatggag
     8581 gagctcgcgc gcggctatgc ctcgatcgcc gaccagtgcg ggctggtcga gctgatcgcg
     8641 accctgctca cccgccacgg cacggccgcg cagaaggcgc ggcatctcgg ccccatcctg
     8701 cgcgccgaga cccgcgtcgc ctactgcatc accgaggctg aggcgggctc ggacgtggcc
     8761 ggcgtcagga ccacggcgga gcgtgcgcgc gacggctggg agctgtcggg gtccaagctg
     8821 tggatccaca acgccccggt cgccgacctc gccttcgtgc tcgcgcgcac cgacaaggag
     8881 gcgggccggc gcggcatgag catcttcatc gtcgactgcg ccgcgcccgg agtgacgcgc
     8941 gggcccaagg agcacaagat gggccagcgc gcctcgcagg tcggcgcgct cggcttcgac
     9001 tcggtggcgc tcgggccgga cgcgctgctc ggcgaggagg gacgcggctt tcacatcatg
     9061 atgagcgcgc tcgacaaggg ccgggtcggc atcgccgcgc tcgcggtcgg catcgcccag
     9121 gcggcgctcg aggcggcgct cgcttacgtg ccccagcggc gccagttcgg ccggccgatc
     9181 gctgatttcc agggcatcca gtggctgctc gccgacatgg ccaaggatgt cgccgccgcg
     9241 cgcctgctca cccaccaggc ggcggtcacg ctcgatgccg gcgggcgggc gacgctcgaa
     9301 tgctcgatgg ccaagtgctt tgccagcgac gccgccgtcc tgcacaccgc caacgccgtc
     9361 cagatctatg gcggcagcgg ctacatccgg ggcttcgagg tcgagcgcct gtaccgcgac
     9421 gccaagatca cgcagatcta cgagggcacc aaccagatcc agcgcaccat catcgcgcgc
     9481 caactgttgg gaggctaaga taatgtgtgc agactcgacc cggccggtgg cgctggtcac
     9541 cggggcgcgg cgcggcatcg gccgggcaac cgcgctggcg ctggcgcggg ccggattcga
     9601 cgtcgccgcc aacgacctcg aggccggcga cggcctggcc gccacggtgc gcgcggtcga
     9661 gagcgaaggt gccagggcgt gcgccgtccc gggcgacatc gccgcgatcg acggccacgc
     9721 ggcgctgctc gacgcggtga caagcgggct cggccggctc gactgcctgg tcaacaacgc
     9781 cggggtctcg gtgctgagcc gcggcgacct gctcgatgtc tcgccggaga gctacgaccg
     9841 ctgcctcgcg gtcaatacgc gcggcacgtt ctttctcacc caggcgttcg cgcgccgcct
     9901 ggttgccgcg ccgccgcccg acgacggcat cccgcgctcc atcgtcacca tcacctcggt
     9961 caacgccgaa gccgcctcga tcaaccgcgg cgagtactgc atctcgaagg cggcgctgtc
    10021 gatggcgtct cgcctgttcg cgttgcgcct ggccgaggtc ggcgccggca tcggggtatg
    10081 ggaggtgcgc ccgggcttca tcgagaccga gatgagcgcg ccatcgaaag cgcgctacga
    10141 cgactacatc agtcgcggca tgaccgcgat ccggcgctgg ggccgaccgg aggacgtggc
    10201 gcaagtgatc gtcaccctcg cctcgggcgg cctcccctac gcggtcggcg aggcgatcca
    10261 cgtcgacggc ggtctgacga tcgccaagtt ctgattccgc gggcggatca aggctcatcg
    10321 cgcagtgcta tgatgccatc gatgcacggc tcccgaccac ttgccctggc gtacgcgctc
    10381 gccatcctgg tcctggggcc gggcgctttc ggcgccgatg cgacctatgc gttcaggggc
    10441 tactcgacca cgctgattcg cggccaggcg ctgttcggcg agatgaccga ggcctgccgg
    10501 cacgacttcg gcggcaaggc gcggctgtgc agcgccggcg aatggtcggc ctcggcggcg
    10561 gcggcggcac cgcgggcggc ggcgtggatc ggccccgacg ccggcgacgg catcggcaac
    10621 gaccagtgcc gcctggcgcc ggcgcgcgga ctaggcctgg cgctgttccc ggacggccgc
    10681 ctggtcggca tctcctgcga caggttcagg ccggtcacct gctgcacccc gcgcgttcgc
    10741 tgagcaccgc acggcgtcgc ggagacaaga aacaaaaagg gccgcagcat gtggcgggga
    10801 ggtttcgccg ctgcggccca gtgccagagg gaggacactc acgcactctc gcgcggcagc
    10861 ggctcttcgt ctgtgaccgt ggtcactctc gcgttgcgcg cgcgcaacag cggg
//
LOCUS       DASWHU010000303        15206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_168443, whole genome shotgun sequence.
ACCESSION   DASWHU010000303 DASWHU010000000
VERSION     DASWHU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168443"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGB88_11195"
     CDS             complement(<1..596)
                     /locus_tag="VGB88_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9648056.1"
                     /translation="MIERAHVVGAGLAGLAAALGLARAGVAVSLYDAAGHAGGRCRSL
                     RDRALGCEIDNGNHLVMAGNRATFAYLDEIGARDRVVGLAPAAFPFLDLDTGERWTVR
                     PNPGPLPWWPLAPRRRVAGTRAADYLAGLKLAFAGAEDTVAGMLARPETLYRRLWQPL
                     ATSALNTDPEHGAARLLWPVLRDTFLRGEAWCRPYVADR"
     gene            complement(607..1479)
                     /gene="hpnD"
                     /locus_tag="VGB88_11200"
     CDS             complement(607..1479)
                     /gene="hpnD"
                     /locus_tag="VGB88_11200"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX9648057.1"
                     /translation="MTAAPDAEAVAAEARAVVARASTSFYWAMRLLPRPKREAMFALY
                     AFCRAVDDIADDPGLVEDKLARLDAWRRDLDTLYRGATPRHAIARALADPIARFALPR
                     APFDEVLRGMESDARGPVQAPSMAELETYCARVAGAVGLQSVRIFGCTDAHADDFALA
                     TGHALQLTNILRDIRDDAAAGRLYLPREALAAAGVEARDPVGVLRDPGLGEACAWLAE
                     RARRRYREADAIRSRMAAADAHSLLPAVIMAAIYRRLLRRIVARGWGAARAPVALGGA
                     EKLWIALRCLAAGG"
     gene            complement(1476..2351)
                     /gene="hpnC"
                     /locus_tag="VGB88_11205"
     CDS             complement(1476..2351)
                     /gene="hpnC"
                     /locus_tag="VGB88_11205"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003616600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX9648058.1"
                     /translation="MAQPSHMPVGVSGRAVEAAASRTADAENFPVGSWLLPRRLRRHI
                     ALYYAFARAADDIADDPDRAAERKVAELDAMDAALSGAKAATSPASAKAVRLAASLAE
                     TGVPIAHGAELLRAFRQDATKLRYTDWAELMDYCRYSAAPVGRYLLDLHGESRATWGP
                     SDALCASLQVLNHIQDCRADYQRLDRVYLPLDAFAAAGAAVGDLDRPAASPELRAVLD
                     RCLDGCEALNREARALPGLIRDRRMRMEAAVIVQIAHRLCALLRGADPLARRIELGPA
                     GKLACLAAGVWTALR"
     gene            complement(2819..4276)
                     /locus_tag="VGB88_11210"
     CDS             complement(2819..4276)
                     /locus_tag="VGB88_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HEX9648059.1"
                     /translation="MTRGSPSRLAGCARACAAALALAAAVVPGAGPLGAADLMLDEIQ
                     QTRIDEAVRAFRMGDYAPALDRLGVLAEEGVADAQYYLGFMNAQGLGVPQDFVAAASW
                     YQEAAGQGHAQAQNHLGLLYFEGSGVPRSFLDAFIYFELAAAAGNEEAANNRLIVARK
                     MTSAQITEAQKAAGQIIAAERATEAVVVRPKRTATGVAITGDGLVLTLAGAIEGCAEP
                     TLRSDAGVMAAAHLIAADEFNRLVLLAGLGPLPAASLRAGPLEAGEAVTVVGFGHDQI
                     EAPPAELRAAQVLRDPALARVDARFFQLDHMLAPGDLGAPVFDAAGRLAALAVPGLDA
                     RQVADVVGRATAAGFALRADLATLLLDANGYVYDVGAADPPPAAEGLDSRLRAVTVAV
                     ECWREDESWPDSTQAATSPGGRRAGGRRRAMGARPRCSFAISAAASINRAASCRCSTA
                     TARRSRRARSAAASARAGRNPGSATRSSPTGWSAG"
     gene            4396..4875
                     /locus_tag="VGB88_11215"
     CDS             4396..4875
                     /locus_tag="VGB88_11215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648060.1"
                     /translation="MAKAASKVVPSAERGHRLVVSRADGATYETDGLREEFAYRDLGS
                     ADATRGEIHAHIIRAKHLHGGHNGLHRHIVDFQFALVLKGWVSFYYPEHGEIRYQQGD
                     AVTVPGGQLHELREYSEDLELLEITGPAVYDTVKEDGTAMATPGQKVRIGERVARAG"
     gene            4872..5747
                     /locus_tag="VGB88_11220"
     CDS             4872..5747
                     /locus_tag="VGB88_11220"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9648061.1"
                     /translation="MSAAARAVFRGYDRAALDAQYNNRKRFPHYVDRFAAWAAWSRDA
                     RARLDARLDLAFGAGEKERLDVFPAAPAGAPIVMFVHGGYWYSLDKGDYSFVAGGLVP
                     NGVATVVNNYALAPTADMDEIVRQNRAACAWIWRHAAEFGWDRDRIFVAGHSAGGHLA
                     AMMLATDWPSFEPGLPADLVKGASAISGLFELEPIRLSYLNDTLHLDAAMAARNAPLL
                     QTYARPAPLLLVLGEDESEEYHRQSRAMAAMWQRLGYRCELVVPDGLDHFSIVDSLRE
                     PDSDLTRRQLALVRS"
     gene            complement(5761..6687)
                     /locus_tag="VGB88_11225"
     CDS             complement(5761..6687)
                     /locus_tag="VGB88_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9648062.1"
                     /translation="MTAEADIAPGRRSLARLTLERFLRHRMALISAVVLAALAVAALA
                     AGMVEARLGVDANEVDLLARFEAASPAHPLGTDELGRDELVRLLYGGRVSLLVALAAA
                     VISALIGTLIGLVAGYYGGWLDRLLMRLTDTVIALPLLPLLIVLAAADLSKLGIPAEL
                     AASPEVSLYRIVLIISLVGWTTVARLVRGATLSLRERDFVLAAISQGAGGVRIMLRHI
                     LPNAVSPIIVATTLSVGTIILLESVLSFLGLGIQPPLASWGNMLTNAQELIYEAPMLA
                     VWPGLAIFVTVIAFNFLGDGLQDALDPRAIEG"
     gene            complement(6684..7652)
                     /locus_tag="VGB88_11230"
     CDS             complement(6684..7652)
                     /locus_tag="VGB88_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9648063.1"
                     /translation="MTRYVAGRMVESLVVLALMSFAIYALMALMPGDPIDIMVQSDPN
                     LTPADAARLKALYGLDKPIVERYGNWLAAAAEGDFGYSRVHNRPVLEVLGPRLLNTLV
                     LMGLTLVISLAAALPIGIWSALRPYSLADYSINFAALGGISVPVFWLALMLIVVFAVT
                     LGWLPAGGMADIGADQTGLGALWDRIRHLILPVATLSVASIGGLTRYVRASMMEVMRH
                     DYIRTARAKGASPARVVLNHALRNAMIPVVTILALSFGTLFSGALITETMFSYLGMGK
                     LIYDSIIGNDYNMALVSLLFATALVLVGNLLADLCYAWLDPRVSYR"
     gene            complement(7657..8631)
                     /locus_tag="VGB88_11235"
     CDS             complement(7657..8631)
                     /locus_tag="VGB88_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9648064.1"
                     /translation="MSELLIEGVGVVKHFPVRAPGLFGRRTGAVHAVNGVSLVVRRGE
                     TLALIGESGCGKTTLGRVLAGLYPATAGEVRFAGQSLAGLDRAGRRRHRRSVQLIFQD
                     PYGSLDPRMTAGAIIDEPLAIHRLGSAAERRARVAELMRAVGLAPVLADHYPHEFSGG
                     QRQRIGIARALAPDPALVIADEPVSALDVSIQSQILNLLRELQAARGLAYLFITHDLA
                     AVDFLADRVAVMYAGRIVEYGQRAQVLEAPRHPYTVALRAAVPEPGRGKRQLGGALRG
                     EVPNPMALPAGCPFHPRCPKAVDECRRTLPRLAPVAGTPGQFAACHLV"
     gene            complement(8628..9644)
                     /locus_tag="VGB88_11240"
     CDS             complement(8628..9644)
                     /locus_tag="VGB88_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648065.1"
                     /translation="MSDLRQAVLGPPPKRPAPPPVLAVDGLTVEFHTRRGVVRPADDV
                     SYQIARGQTLAVVGESGCGKTVGALAVMGLVDPPGRITAGRVMLRGTDLRLLPEAALR
                     RLRGREMAMIFQEPATALNPVMCIGDQVAEPLILHDRLGPREAWRAAVELLDRVGIPM
                     PARRARDYPHQLSGGMRQRVMIAIALACNPSLLIADEPTTALDVTVQAQILELLLALQ
                     ESYGTAIQYISHDLGVISEIADEVAVMYAGRIVERAPAGQLFEDPRHPYTRGLLATRP
                     RIGVRAKRLPAIPGQVPDLARLASGCAFRDRCALARPHCAERPPALETIAAGHQAACF
                     EVKP"
     gene            complement(9670..11343)
                     /locus_tag="VGB88_11245"
     CDS             complement(9670..11343)
                     /locus_tag="VGB88_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9648066.1"
                     /translation="MTATSTRRAARLALVLAGTLALGAPALAKDEIVIGITQFPSTLH
                     PHINAMLAKSYIISAAMRPLTTYDQDWKMVCVLCTELPTIDNGGAVVETLEDGSQGVA
                     VTYTIQPEARWGDGVPVTSDDALFTWQVGRDEQSGFAGIEGFRRTLSVEVVDDKTFTF
                     HVDRLTYQYNDANGFYLLPRHREAEAFADPAQYRFRTRYDTAPTDPGLYYGPYRVTEV
                     ESGSHVVLEPNPTWYGPKPHFKRVVVRVIENTAALEANLLSGAIDYVAGELGFTVEQA
                     LSFESRNRDRFDVLYKPGLIYEHLDVNLDNPVLADRRVRQALLYALDRDNMTAKLFGG
                     RQPVADSNVNPLDRVHAEEVPRYPYDPDRAVALLEDAGWTELRDGVRRNEAGERLSFP
                     LMTTAGNKSRELVQQVLQSEWRKVGVEAVIRNEPARVFFGETTAKRQNPGLSMYAWLS
                     APESVPLSTLRSDQIPAEANGWSGQNYPGYRNERMDALIDTIERELDAQARLPLWAEL
                     QALYATDLPALPLYFRAEAYAFPKWLEGVRPTGHLVTTTMWIEEWRARD"
     gene            complement(11360..11971)
                     /locus_tag="VGB88_11250"
     CDS             complement(11360..11971)
                     /locus_tag="VGB88_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX9648067.1"
                     /translation="MADRIDYYLALSSPWTYLAGPRFKALARRNKLDVTFRPYNIMHV
                     FGINGTKPVAQRPKPIQANRLRELGRWHAFLGMPLNLKPKFFPVDPTLAGKMLIAAQR
                     TGAAGDKVIDLSFAFLSACWAEERNLADDDTLVAIANDAGFDGGALLKSAKAGAADAE
                     FDRNTEDALANDVFGSPTWMFMGELFWGQDRLEFLTRTVEGGS"
     gene            complement(12057..12914)
                     /locus_tag="VGB88_11255"
     CDS             complement(12057..12914)
                     /locus_tag="VGB88_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9648068.1"
                     /translation="MRRARDVLQGLVPVLVAVAVGYAGVLGAAYLLQRQMVYFPDPRP
                     PDPEVAAALGFEPVTLATADGLALVSWYAPPKSAQHPVLVYLHGNADAIGRRAPKFVP
                     YLDAGFGALLVEYRGYAGNPGTPHEAGLYRDGEAALAWLGRHGIDGPRVVAFGSSLGT
                     GVAIELASRHALGALVLEAPFTSLAEVGARAYPWLPVRVLARDRFDSLAKMARIAAPV
                     LVIHGEADRIVPAAMGRAILEAARGPKAGLFLPSLDHDEVSIEGAPAFVIESLRGFGV
                     APAAAGHGP"
     gene            13028..14110
                     /locus_tag="VGB88_11260"
     CDS             13028..14110
                     /locus_tag="VGB88_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9648069.1"
                     /translation="MFLAACGPLSREAAWLLDDIAAGAGPSRLKDETPAPVPSAVRLA
                     AGGIALDADLYLSPAGARAALVLVPGLARAGRADARLVALAQSLARVRFAVLVPEITG
                     LSDLQVAPAQAGEIAAAAAWLAGRGDLARGRPVGVVAISYAAGPALIAAASPAGRADI
                     GFVVTVGGYYDIESAVTYVTTGAYYASGRWRHGTPNAYGKWVFVNSNAHRLADSRERA
                     ALAAIAERKLDDPEAPIDDLARALRDDGRAVLALVTNDRRRRVAELIAALPEAIRADL
                     AALDPAARPLEDMRARLILIHGRDDTIIPYSESVDLARRLGSARASLYLLDGLWHVDA
                     AFSLGDRLTLWRAAVELLELRDGAER"
     gene            complement(14224..>15206)
                     /locus_tag="VGB88_11265"
     CDS             complement(14224..>15206)
                     /locus_tag="VGB88_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX9648070.1"
                     /translation="HAVERAVVPPGVAFDIYNERDPAAIPDRVWQRCDGMVTGIRMPI
                     DGAVIGRLESCRIITRLGVGYDLIDVAAAGARGIAVCNVPDYGTTEVADHALALLLSL
                     TRGVARYNEAYRADAEAGWNYQIPPTVTRHGGKTFGVIGLGRIGTAAAMRAKAFGMRV
                     VFYDPYVPDGQELALGISRVRALADLLAQADFVTVHAPLTEDTRHLIDARAVAAMKPG
                     LILVNTARGPIVDLDAAYDGLKSGKLGAVGLDVFDLEPPPADHPLIRAWRAGEDWIAG
                     RCLITPHAAFYSPTSVVDLREKAVMTCVDFLTAGVLRNCVNRALLKDGPG"
BASE COUNT         2193 a         5427 c         5615 g         1971 t
ORIGIN      
        1 cggtcggcga cgtacggccg gcaccaggcc tcgccgcgca ggaaggtgtc cctgagcacc
       61 ggccacagca gccgcgccgc gccgtgctcg ggatcggtgt tgagcgccga ggtggcgagc
      121 ggctgccaca gccgccggta cagcgtctcc gggcgcgcca gcatccctgc gaccgtgtcc
      181 tcggcgccgg cgaaagcgag cttgagcccg gccagataat ccgcggcacg ggtgccggcg
      241 acgcgccgcc gcggcgccag cggccaccac ggcagcgggc ccgggttcgg gcgcacggtc
      301 cagcgctcgc cggtgtcgag gtcgaggaac gggaacgccg ccggggcgag cccgaccacg
      361 cggtcgcgcg cgccgatctc gtcgagatag gcgaaggtcg cgcggttgcc ggccatcacc
      421 aggtggttgc cgttgtcgat ctcgcagccg agcgcgcggt cgcgaagcga gcggcagcgc
      481 ccgccggcat ggccggcggc atcgtagagc gacaccgcga cccccgcgcg ggcaaggccg
      541 agcgccgcgg cgaggccggc gaggccggcg ccgacgacgt gggcgcgctc tatcaccgtc
      601 ggctgctcag ccgccggcgg cgaggcagcg cagcgcgatc cacagcttct cggcgccgcc
      661 gagcgcgacc ggcgcgcgcg ccgcgcccca gccgcgggcg acgatgcgcc ggagcaggcg
      721 gcggtagatc gcggccatga tcaccgccgg cagcaggctg tgcgcgtcgg ccgccgccat
      781 gcggctgcgg atcgcgtcgg cctcgcggta gcggcggcgc gcgcgctcgg ccagccaggc
      841 gcaggcctcg ccgagccccg ggtcgcgcag cacgccgacc gggtcgcggg cctcgacgcc
      901 ggccgccgcg agcgcctcgc gcggcaaata gagccgcccc gcggcggcgt cgtcgcggat
      961 gtcgcgcagg atgttggtga gctggagcgc gtgcccggtg gcgagcgcga agtcgtcggc
     1021 gtgggcgtcg gtgcagccga agatgcgcac cgattgcagc ccgaccgcgc cggcgacgcg
     1081 cgcgcaatag gtctcgagct cggccatcga cggtgcctgc accggcccgc gggcgtcgct
     1141 ctccatgccg cgcagcacct cgtcgaacgg cgcccgcggc agggcgaagc gcgcgatcgg
     1201 gtcggcgagc gcgcgcgcga tggcatggcg cggcgtggcg ccgcggtaga gcgtgtcgag
     1261 gtcgcggcgc caggcgtcga gccgggcgag cttgtcctcg accaggcccg ggtcgtcggc
     1321 gatgtcgtcg accgcgcggc agaaggcata gagcgcgaac atcgcctcgc gcttgggccg
     1381 cggcaagagc cgcatcgccc agtagaacga cgtgctcgcg cgcgcgacca cggcgcgggc
     1441 ctcggccgcg accgcctcgg cgtcgggcgc cgcggtcacc tgagcgccgt ccacacgccc
     1501 gccgccaggc aggcgagctt gccggccggg ccgagctcga tgcgccgcgc cagcgggtcg
     1561 gcgccgcgca ggagcgcgca cagccggtgc gcgatctgga cgatcaccgc cgcctccatg
     1621 cgcatccgcc ggtcccggat caggccgggc agcgcccgcg cctcgcggtt gagcgcctcg
     1681 cagccgtcga gacagcggtc gagcaccgcg cgcagctccg ggctcgccgc cggccggtcc
     1741 aggtccccga ccgcggcgcc ggcggcggcg aacgcatcga gcggcaggta gacccggtcg
     1801 aggcgctggt agtcggcccg gcagtcctgg atgtggttga gcacctggag cgaggcgcag
     1861 agcgcgtccg agggccccca ggtcgcccgc gactcgccgt gcaagtcgag cagatagcgc
     1921 ccgaccggcg ccgccgaata gcggcagtag tccatcagct cggcccagtc ggtgtagcgc
     1981 agcttggtcg cgtcctggcg gaaggcgcgc agcagctcgg cgccgtgggc gatcggcacg
     2041 cccgtctcgg ccaggcttgc ggccaggcgg acggccttgg cggatgccgg cgaggttgcg
     2101 gccttcgccc ccgacaaggc cgcgtccatc gcgtccagct cggcgacctt gcgctcggcc
     2161 gcccggtcgg ggtcgtcggc gatgtcgtcg gcggcgcggg cgaaggcgta atagagcgcg
     2221 atgtgccggc gcagtcgccg cggcagcagc cacgagccga ccgggaagtt ctccgcgtcg
     2281 gcggtgcgcg aggccgccgc ctcaacggcg cgcccgctga cgccgaccgg catgtgcgat
     2341 ggctgtgcca acggcttcgt ccgtgacgcc gatcccccga tccggcctac ctaatagccg
     2401 gggcgttccc cgctcacaag acgcacgccc gggcggcacc aaacgaggca aaaaattgcc
     2461 ccccttcccg gggggaacgg gaaggggggc acacaagtcc gcggcggggc gggggacatg
     2521 gagggatacc cgccgctttg gtaagggagg agttgactcg aacgggccat cccattgcgg
     2581 gggcgcgttc gactgcctcg ggcgcatata tgggtcaatc gaacgtttaa atcaagtgat
     2641 tgttaatccc gatccgaggc caagcgcgaa agacaaaacg accgttcatc aatacgttag
     2701 cgaatcgacc tcaattccgg tcgaaaggcg cccgacatca caataatttg aggggctcgg
     2761 ggcccgcggg gttatcgcgg ggttgcgggc tcggacgcca cggcgcggcc gtgcgcggtc
     2821 agccggcaga ccagccagtc gggcttgatc gggttgccga accagggttc cgcccagccc
     2881 tggcggatgc agctgcggat cgtgcgcggc gagatcgccg cgccgtcgcg gtcgaacagc
     2941 ggcagcttgc cgcccggttg atcgaggccg cggcagagat agcgaagctg caccgcggac
     3001 gggcgcccat cgcccgccgc ctgccgccgg cgcgccggcc gccgggcgag gtggccgcct
     3061 gcgtcgagtc cggccacgac tcgtcctccc tccagcactc gaccgcgacc gtcaccgcgc
     3121 gcagtcgcga gtccagtccc tcggccgcgg gcggcgggtc ggccgcgccg acatcataga
     3181 cgtagccgtt ggcgtccaac aagagcgtgg ccaggtcggc gcggagcgcg aagccggccg
     3241 cggtcgcccg gccgaccacg tccgcgacct ggcgcgcatc caggcccggc acggccaggg
     3301 cggcgagccg gcccgcggcg tcgaacaccg gcgccccgag gtcgccggga gcgagcatgt
     3361 ggtcgagctg gaagaagcgc gcatcgacgc gcgcgagcgc cgggtcgcgc aggacctggg
     3421 ccgcgcgcag ctcggccggc ggggcctcga tttggtcgtg cccgaagccg accacggtga
     3481 ccgcctcgcc ggcctcgagc ggcccggcgc gcaggcttgc cgccggcagc ggcccgaggc
     3541 cggcgagcag caccagccgg ttgaactcgt ccgcggcgat caggtgtgcc gccgccatca
     3601 cgcccgcgtc gctgcgcaag gtcggctcgg cgcagccctc gatcgccccg gccaaggtca
     3661 gcaccagccc gtcgccggtg atcgccacgc cggtcgcggt gcgctttggc cgcaccacca
     3721 ccgcctccgt tgcgcgctcg gcggcgataa tctggcccgc cgccttctgc gcctcggtga
     3781 tctgcgccga ggtcatcttg cgggcgacga tcaggcggtt gttggccgcc tcctcgttgc
     3841 ccgcggcagc ggccagctcg aaatagatga acgcgtcaag gaacgagcgc ggcacgcccg
     3901 agccctcgaa atagagcagc cccagatggt tctgcgcctg ggcgtggccc tggccggcgg
     3961 cctcttgata ccacgacgcc gcggcgacga agtcctgggg cacgccgaga ccctgggcgt
     4021 tcatgaagcc gaggtagtac tgggcgtcgg cgacgccctc ctcggccagg acgccgagcc
     4081 ggtcgagcgc cggcgcgtag tcgcccatcc ggaacgcccg caccgcctcg tcgatgcggg
     4141 tctgctggat ctcgtcgagc atcaggtcgg cggcgccaag cggcccggcg ccggggacca
     4201 cggcagccgc gagcgcgagc gccgccgcgc aggcgcgcgc gcatccggca agacgtgacg
     4261 gggagccccg ggtcatggcc tcgcccgagc gtaggcccgc gattgctgac aaccagttaa
     4321 cgcgcgcgcc catccggggt agtgttggcg ccggcgccgt cgcggcgcgc agcttggcga
     4381 gcagggagaa cgaccatggc aaaggcggca tccaaggtgg tgccctcggc ggagcggggc
     4441 caccggctgg tggtcagccg cgcggacggc gcgacctacg agaccgacgg cctgcgcgag
     4501 gagttcgcct atcgcgacct tggctcggcc gacgctacgc gcggcgagat ccacgcccac
     4561 atcatccgcg ccaagcacct gcacggcggc cataacggct tgcaccgcca catcgtcgac
     4621 ttccagttcg cgctggtgct caagggctgg gtgagcttct actacccgga gcacggcgag
     4681 atccgctacc agcagggcga tgccgtcacc gttcccggcg gccagctgca cgagctgcgc
     4741 gagtactccg aggacctgga gctgctggag atcaccggcc ccgcggtcta cgacaccgtc
     4801 aaggaggacg gcacggcgat ggcgacgccg ggccagaagg tgcgcatcgg cgagcgcgtc
     4861 gcccgcgccg gatgagcgcc gcggcacgcg cggtctttcg cggctacgac cgggcggcgc
     4921 tcgacgccca gtacaacaac cgcaagcgct ttccccacta tgtcgaccgg ttcgcggcct
     4981 gggccgcgtg gagccgggat gcgcgcgcac ggctggacgc gcgcctcgac ctcgccttcg
     5041 gcgccggcga gaaagagcgg ctcgacgtct ttcccgccgc gccggccggc gcgccgatcg
     5101 tgatgttcgt ccacggcggc tactggtact cgctggataa gggcgactac agcttcgtcg
     5161 ccggcggcct ggtgccgaac ggggtcgcga ccgtggtcaa caactacgcg cttgcgccca
     5221 ccgccgacat ggacgagatc gtgcgccaga accgcgccgc ctgcgcctgg atctggcgcc
     5281 acgccgcgga gttcggctgg gatcgcgatc gcattttcgt cgccggccat tccgcgggcg
     5341 ggcatctggc ggcgatgatg ctcgccaccg actggccaag cttcgagccg ggcctgccgg
     5401 ccgacctggt caagggcgca tccgcgatca gcggcctgtt cgagctcgag ccgatccggc
     5461 tgtcctacct caacgacacc ctccacctcg acgccgccat ggcggcgcgc aatgcgccgc
     5521 tgctccagac ctatgcgcgg ccggcgccac tcctgctggt gctgggcgag gacgagtcgg
     5581 aggagtacca ccgccagtcg cgcgccatgg cggcgatgtg gcagcggctc ggctatcggt
     5641 gcgagctggt ggtcccggac ggcctcgacc acttctcgat cgtcgactcg ctccgcgagc
     5701 ccgactccga cctcacacgc cgccagctcg ccctggtccg cagctagggc gcgcgcccgc
     5761 tcagccttcg atcgcgcgcg ggtcgagggc gtcctggagg ccgtcgccga ggaagttgaa
     5821 cgcgattacg gtgacgaaga tcgcgagccc cggccacacc gccagcatcg gcgcctcgta
     5881 gatcagctcc tgggcgttgg tcagcatgtt gccccagctc gcgagcggcg gctggatgcc
     5941 gaggccgagg aagctcagca ccgattccag caggatgatg gtgccgaccg acaaggtggt
     6001 ggcgacgatg atcggcgaga cggcgttggg caggatgtgg cggagcatga tgcgcacccc
     6061 gccggcaccc tggctgatcg cggcgagcac gaagtcgcgc tcgcgcaagg acagcgtcgc
     6121 gccgcgcacc aggcgcgcga ccgtggtcca gccgaccagc gagatgatca gcacgatccg
     6181 gtacaggctc acctcgggcg aggccgcgag ctcggccggg atgccgagct tcgacaggtc
     6241 cgccgcggcg agcacgatca gcagcggcag cagcggcaac gcgatcaccg tgtcggtgag
     6301 ccgcatcagc agcctgtcga gccagccgcc gtaatagccg gcgacgagcc cgatcagggt
     6361 gccgatcagc gccgagatca ccgctgcggc gagcgccacc aggagcgata cccgcccgcc
     6421 atagagcagg cgcaccagct cgtcgcggcc gagctcgtcg gtgccgagcg gatgcgcggg
     6481 cgaggcggct tcgaagcgcg ccagcaggtc gacctcgttg gcatcgaccc caagccgcgc
     6541 ctcgaccatc ccggcggcga gcgccgcgac ggcgagggcc gccagaacca ccgcactgat
     6601 gagcgccatg cgatggcgca ggaagcgctc gagggtgagg cgtgcaagcg accggcgccc
     6661 gggggcgatg tccgcttccg cggtcatcgg tagctcaccc tggggtcgag ccaggcgtag
     6721 cacaggtcgg cgagcaggtt gccgaccagc accagcgcgg tcgcgaacaa gaggctcacc
     6781 agcgccatgt tgtagtcgtt gccgatgatc gagtcgtaga tcagcttgcc catgccgaga
     6841 taggagaaca tggtctcggt gatcagcgcg cccgagaaca gggtgccgaa cgacaaagca
     6901 aggatggtga ccaccgggat catggcgttg cgcagcgcgt ggttcagcac cacgcgcgcg
     6961 ggcgatgcgc ccttggcgcg ggcggtgcgg atgtagtcgt ggcgcatcac ctccatcatc
     7021 gaggcgcgca cgtagcgggt cagcccgccg atgctggcga cgctcaaggt ggccaccggc
     7081 aggatcaggt ggcggatgcg gtcccacagc gcgccgaggc cggtctggtc ggcgccgatg
     7141 tccgccatgc cgcccgccgg cagccagccg agggtgaccg cgaacaccac gatcagcatc
     7201 agtgccagcc agaacaccgg caccgagatg ccgccgaggg cggcgaagtt gatcgagtag
     7261 tcggccaacg aatagggccg cagcgccgac cagatgccga tcggcagcgc cgcggcgagc
     7321 gagatgacca gggtcaggcc catcagcacg agcgtgttca ggagcctggg ccccagcacc
     7381 tcgagcaccg ggcggttgtg gacgcgcgag tagccgaagt cgccctccgc cgccgccgcc
     7441 agccagttgc cgtagcgctc gacgatcggc ttgtcgagcc cgtacagcgc cttcagccgg
     7501 gcggcgtcgg caggcgtgag gttcgggtcc gactggacca tgatgtcgat cgggtcgccc
     7561 ggcatcagcg ccatcagcgc gtagatcgcg aacgacatca gcgccagcac caccagcgat
     7621 tcgaccatgc ggccggcgac gtagcgggtc atcggctcac accaggtggc aggcggcgaa
     7681 ctggccgggg gtgcccgcga ccggcgcgag ccggggcagc gtgcgccggc actcatcgac
     7741 cgccttcgga cagcgcgggt ggaacgggca gcccgcgggc aacgccatcg gattcggcac
     7801 ctcgccgcgc agcgcacccc cgagctggcg cttgccgcgc ccgggctcgg gcaccgccgc
     7861 gcgcagcgcc accgtgtagg gatggcgcgg cgcttccagc acctgcgcgc gctggccgta
     7921 ctcgacgatc cgcccggcat acatcaccgc cacccggtcg gcgaggaagt cgaccgcggc
     7981 gaggtcgtgg gtgatgaaca ggtaggcgag cccgcgcgcc gcctgcagct cgcgcaacaa
     8041 gttgaggatc tggctctgga tcgacacgtc gagcgccgag accggctcgt cggcgatgac
     8101 cagcgccggg tcgggcgcga gcgcgcgcgc gatcccgatc cgctggcgct ggccgcccga
     8161 gaactcgtgc gggtaatggt cggcgagcac cggcgcgagg ccgaccgcgc gcatcagctc
     8221 ggcgacgcgc gcgcgccgct cggcggcgct gccgaggcga tggatggcga gcggctcgtc
     8281 gatgatcgcc cccgccgtca tgcgcggatc gagcgagccg taagggtcct ggaagatcag
     8341 ctgcaccgag cggcggtggc ggcgccggcc ggcgcggtcg aggccggcca acgattggcc
     8401 ggcgaaccgt acctcgcccg cggtcgccgg gtagagcccg gcgagcacgc gcccgagggt
     8461 cgtcttgccg cagccgctct cgccgatcaa ggccagcgtc tcgccgcggc ggacgaccag
     8521 cgagacaccg ttgaccgcgt gcacggcgcc ggtcctgcgc ccgaacaggc cgggcgcgcg
     8581 caccgggaaa tgcttgacca cgccgacgcc ctcgatcagc agctcgctca cggcttgacc
     8641 tcgaagcagg cggcctggtg gccggcggcg atggtctcga gcgcgggcgg ccgctcggcg
     8701 cagtgcgggc gcgcgagcgc acagcggtcg cggaacgcgc agcccgaggc gagccgggcg
     8761 aggtccggga cctggccagg gatcgcgggc agccgcttcg cgcgcacccc gatccgcggc
     8821 cgggtggcga gcaggccgcg ggtgtagggg tggcgcgggt cctcgaacag ttgcccggcg
     8881 ggcgcgcgct cgacgatccg cccggcatac atgaccgcga cctcgtcggc gatctccgag
     8941 atcacgccga ggtcgtggct gatgtactgg atcgccgtgc cgtaggactc ctgaagcgcg
     9001 agcaggagct cgaggatctg cgcctgcacg gtgacgtcga gcgccgtggt cggctcgtcg
     9061 gcgatcagca gcgacgggtt gcaggcgagc gcaatcgcga tcatcacccg ctggcgcatg
     9121 ccccccgaaa gctgatgcgg atagtcgcgt gcgcgcctcg ccggcatcgg gatgccgacc
     9181 cggtccagca gctcgaccgc cgcccgccac gcctcgcgcg ggccgaggcg atcatgcagg
     9241 atcagcggct cggcgacctg gtcgccgatg cacatcaccg ggttgagcgc ggtcgccggc
     9301 tcctggaaga tcatcgccat ctcgcgcccg cgcagccggc ggagcgcggc ctcgggcaac
     9361 agacgcaggt cggtgcccct gagcatcacc cggccggcgg tgatccgccc cggcggatcg
     9421 actaggccca tcaccgcaag cgcgcccacg gtctttccgc agccgctctc gccgaccaca
     9481 gcgagcgtct gcccgcgcgc gatctggtag ctgacgtcgt cggcggggcg caccacgccg
     9541 cgccgggtgt ggaactcgac cgtcagccca tcgaccgcga gcaccggcgg cggcgccggc
     9601 ctcttgggcg gcgggccgag aaccgcttgg cgcaggtcgg acatcgctcg cgtctgctcc
     9661 gcgcttaccc taatcgcgcg cgcgccactc ctcgatccac atggtggtgg tgaccagatg
     9721 gccggtcggg cgcacgccct caagccactt ggggaaggcg taggcctcgg cgcggaagta
     9781 gagcggcagc gccggcaggt cggtggcata cagcgcctgc agctcggccc acagcggcag
     9841 ccgcgcttgc gcgtcgagct cgcgctcgat cgtgtcgatc agcgcgtcca tgcgctcgtt
     9901 cctgtagccc ggatagttct ggcccgacca gccgttggcc tcggccggga tctggtccga
     9961 gcgcagggtc gacagcggca cgctctctgg cgcgctcagc caggcataca tcgacaggcc
    10021 cgggttctgg cgcttggccg tggtctcgcc gaagaacacc cgcgccggct cgttgcggat
    10081 caccgcctcg acgccgacct tgcgccactc gctctgcagc acctgctgga ccagctcgcg
    10141 cgacttgttg ccggcggtgg tcatcagcgg gaagctcagg cgctcgccgg cttcgttgcg
    10201 gcgcacgccg tcgcggagct cggtccagcc ggcgtcctcg agcagggcca ccgcccggtc
    10261 cgggtcatag gggtatctgg gcacctcctc ggcgtggacc cggtcgagcg ggttgacgtt
    10321 cgagtccgcc accggctggc ggccgccgaa cagcttggcg gtcatgttgt cgcggtcgag
    10381 cgcgtagagc agcgcctggc gcacccgccg gtcggcgagc accgggttgt cgagattgac
    10441 gtccaggtgc tcgtagatca gcccgggctt gtacaggacg tcgaagcggt cgcggttgcg
    10501 gctctcgaac gacagcgcct gctcgaccgt gaagccgagc tcgccggcga cgtagtcgat
    10561 cgcgcccgac aacaggttgg cctcgagcgc ggcggtgttc tcgatcacgc gcacgaccac
    10621 gcgcttgaaa tggggcttgg gcccgtacca ggtcgggttg ggctcgagca ccacgtgcga
    10681 gcccgactcg acctcggtca cccgataggg gccgtaatag agcccggggt cggtgggcgc
    10741 ggtgtcgtag cgggtgcgga agcggtactg cgccggatcg gcgaacgcct cggcctcgcg
    10801 gtggcgcggc agcaggtaga agccgttggc gtcgttgtac tgataggtca gccgatcgac
    10861 atggaaggtg aaggtcttgt cgtcgaccac ctcgaccgag agcgtgcgcc ggaagccctc
    10921 gatgccggcg aagccgctct gctcgtcgcg gccgacctgc caggtgaaca gggcgtcgtc
    10981 cgaggtcacc ggcacgccgt cgccccagcg cgcctcgggc tggatggtgt aggtgacggc
    11041 gacgccctgg ctgccgtcct cgagcgtctc gaccaccgcg ccgccgttgt cgatggtcgg
    11101 cagctcggtg cacagcacgc acaccatctt ccagtcctgg tcgtaggtgg tcagcggccg
    11161 catcgcggcg ctgatgatgt aggacttggc cagcatggcg ttgatgtgcg ggtgcagggt
    11221 cgaggggaac tgggtgatgc cgatcacgat ctcgtccttg gccagcgccg gcgcgcccag
    11281 cgcgagcgtg ccggcgagga cgagcgcgag ccgcgccgcg cgccgtgtgg acgtcgcggt
    11341 catcgcccgt gcgctcggat caggacccgc cctcgaccgt gcgggtcagg aactcgagcc
    11401 ggtcctggcc ccagaacagc tcgcccatga acatccaggt cggcgagccg aacacgtcgt
    11461 tggcgagcgc atcctcggtg ttcctgtcga actcggcgtc ggccgcgccg gccttggccg
    11521 acttcaacag cgccccgccg tcgaagcccg cgtcgttggc gatcgcgacc agggtgtcgt
    11581 cgtcggcgag gttgcgctcc tccgcccagc aggcgctcag gaacgcgaac gacaggtcga
    11641 tcaccttgtc gcccgcggcg ccggtgcgct gcgcggcaat cagcatcttg cccgcgagcg
    11701 tcgggtcgac cggaaagaac ttgggcttga ggttgagcgg catgccgagg aacgcgtgcc
    11761 aacggccgag ctcgcgcagc cggttggcct ggatcggctt cggccgctgg gcgaccggct
    11821 tggtgccgtt gatcccgaac acgtgcatga tgttgtacgg cctgaaggtg acgtcgagct
    11881 tgttgcgccg cgccagcgcc ttgaaccgcg gcccggcgag gtaggtccag ggcgaggaca
    11941 atgccaggta atagtcgatc ctgtcggcca tggcggaagc ggtctcctgt cggtcgccgg
    12001 aatgcgggac ggtgtcgtga cggcgccgga atgataggcg gcgcctcgat ccccgatcaa
    12061 gggccatggc cggccgcggc cggcgcgacg ccgaagccgc gcagcgactc gatcacgaac
    12121 gcgggcgcgc cctcgatcga cacctcgtca tggtcgagcg agggaaggaa cagccccgcc
    12181 ttgggccccc gcgccgcctc caggatcgcc cggcccatgg cggccggcac gatgcggtcg
    12241 gcctcgccgt gaatgaccag caccggcgcc gcgatgcgcg ccatcttcgc cagcgagtcg
    12301 aagcggtcgc gcgccagcac gcgcaccggc agccacggat aggcgcgcgc gccgacctcg
    12361 gcaagcgagg tgaacggcgc ctcgagcacc agcgcgccga gcgcatggcg gctggcgagc
    12421 tcgatcgcga cgccggtgcc gagcgagctg ccgaacgcca ccacgcgcgg cccgtcgatg
    12481 ccgtggcggc cgagccaggc gagcgcggcc tcgccgtcgc ggtacaggcc cgcctcgtgc
    12541 ggggtgccgg ggttgccggc atagccccgg tactcgacca gcagagcgcc gaagccggcg
    12601 tcgagataag gcacgaactt cggtgcccgc cggccgatgg catcggcgtt gccgtgcagg
    12661 tacaccagca ccggatgctg cgccgacttg ggcggcgcgt accacgacac cagcgcaagc
    12721 ccgtcggcgg tcgccagcgt caccggctcg aagccgagcg cggcggcgac ctcggggtcg
    12781 ggcgggcgcg ggtcggggaa atagaccatc tggcgctgga gcagataggc cgcgccgaga
    12841 acgccggcgt agccgacagc caccgcgacc agcacgggaa ccagaccctg aagcacgtcg
    12901 cgcgcccttc gcatccgtcc cacgctcgcg cgggccgacc gggatgaccc tagcaaaccg
    12961 cttggccgtc tgtctccgcc ggccggggag cgcggtcctg cgcgccggtc cggcgctcgt
    13021 cctctgtgtg ttcctggccg cctgcggccc gctttcgcgc gaggcggcgt ggctgctcga
    13081 cgacatcgcg gcgggcgccg ggccgagccg gctcaaggac gagaccccgg cgccggtgcc
    13141 gagcgcggtg cggctcgcgg ccggcgggat cgcgctcgac gccgacctgt acctgtcgcc
    13201 cgcgggcgcg cgcgccgcgc tggtgctggt gccggggctt gcgcgcgcgg gccgggcgga
    13261 tgcgcgcctg gtcgcgctcg cgcaaagcct ggcgcgggtg cggttcgcgg tgctggtgcc
    13321 cgagatcacg gggctcagcg acctccaggt ggcgccggcc caggctgggg agatcgccgc
    13381 cgccgcggcc tggctcgcgg ggcgcggcga cctcgcccgc ggccgccccg tgggcgtggt
    13441 cgcgatcagc tatgccgccg ggccggctct gatcgcggcg gcctcgcccg ccgggcgcgc
    13501 ggacatcggc ttcgtggtca cggtgggcgg ctattacgac atcgagagcg cggtgaccta
    13561 tgtcaccacc ggcgcctact acgcctccgg gcgctggcgc cacggcacgc ccaacgccta
    13621 cggcaagtgg gtgttcgtca acagcaacgc ccaccgcctg gccgattcgc gcgagcgcgc
    13681 ggcgctggct gcgatcgccg agcgcaagct cgacgacccg gaggcgccga tcgacgacct
    13741 cgcgcgagcg ctccgcgacg acgggcgggc ggtgctcgcg ctggtcacca acgaccgccg
    13801 ccggcgcgtc gccgagctca tcgccgcgtt gcccgaggcg atccgcgccg acctggcggc
    13861 gctcgatccg gcggcgcggc cgctcgagga catgcgtgcg cggctgatcc tgatccacgg
    13921 ccgcgacgac accatcatcc cctattccga gagcgtcgac ctcgcccggc ggctcggctc
    13981 ggcgcgcgcc tcgctctatc tgctcgacgg cttgtggcac gtggacgccg cgttctcgct
    14041 cggcgaccgc ctgaccttgt ggcgggccgc ggtcgagctg ctcgagctgc gcgacggcgc
    14101 ggagagatag gccacccccc acctgcctcc ccccgccagc ggggggaggt tcaacgttaa
    14161 atccctcccc cgtgggcggg ggagggtttg ggtggggggc gagcgcacgc gagccgaagc
    14221 cggtcaaccg ggcccatcct tcagcagggc gcggttgacg cagttgcgca agacgccggc
    14281 agtgaggaag tcgacgcagg tcatcaccgc cttctcgcgc aaatcgacga cgctggtcgg
    14341 gctgtagaag gcggcgtgcg gggtgatcag gcagcgcccg gcgatccagt cctcgccggc
    14401 gcgccaggca cggatcagcg gatggtcggc gggcggcggc tcgaggtcga acacgtcgag
    14461 gccgaccgcc cccagcttgc cgctcttgag gccgtcatag gcggcgtcga ggtcgacgat
    14521 cgggccgcgc gcggtattga ccaggattag gcccggcttc atcgcagcca ccgcgcgcgc
    14581 gtcgatcagg tggcgggtat cctcggtcag cggcgcgtgg acggtgacga agtcggcctg
    14641 cgcgagcagg tcggcgagcg cgcgcacgcg actgataccg agcgcgagct cctggccgtc
    14701 gggcacgtag gggtcgtaga acaccacccg catgccgaat gccttggcgc gcatcgccgc
    14761 cgcggtgccg atccgcccca gcccgatgac gccgaaggtc ttgccgccgt ggcgggtgac
    14821 ggtgggcggg atctggtagt tccagccggc ctcggcgtcg gcccggtagg cctcgttgta
    14881 gcgggcgacg ccgcgggtga gcgacagcag cagcgcgagc gcatggtcgg cgacctcggt
    14941 cgtgccgtag tcgggcacgt tgcacaccgc gatgccgcgc gcgccggcgg cggcgacgtc
    15001 gatcaggtcg tagccgacgc cgagccgggt gatgatccgg catgactcga gccggccgat
    15061 aacggcgccg tcgatcggca tgcggatgcc ggtgaccatg ccgtcgcagc gctgccagac
    15121 ccggtccggg atcgccgccg ggtcgcgctc gttgtagatg tcgaaggcca cccccggcgg
    15181 caccaccgcg cgctcgaccg catgct
//
LOCUS       DASWHU010000304         8167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2766787, whole genome shotgun sequence.
ACCESSION   DASWHU010000304 DASWHU010000000
VERSION     DASWHU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766787"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..540)
                     /locus_tag="VGB88_11270"
     CDS             complement(37..540)
                     /locus_tag="VGB88_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9648071.1"
                     /translation="MALASFRLRAAQGGAAAGAVKGSLDRRPAPAMMHAMARRPRHPP
                     SEDELREIAAAVLATIPEMLRRQAGNVVVQVTDFADDEVLDDMGLDSPFDLMGLYQGV
                     AMTERSVSQPAPEPDMVYLYRRPLLDYWCETGADLAEVVRYVLIHEIGHHFGYSDEEM
                     DELQGEP"
     gene            539..829
                     /locus_tag="VGB88_11275"
     CDS             539..829
                     /locus_tag="VGB88_11275"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9648072.1"
                     /translation="MSEKITGAALDAALAGLAGWARVAGREAISKSYRFADFNAAFGW
                     MTRAALMAERMDHHPEWFNVYNRVEVTLASHDVGGVSARDIALARFLDSLPG"
     gene            840..1304
                     /locus_tag="VGB88_11280"
     CDS             840..1304
                     /locus_tag="VGB88_11280"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEX9648073.1"
                     /translation="MAGTRASVPRPALVLGVAGAAPFVAGALAMLVGPGWLKLTAYVY
                     LVNYAAVALAFVGAVHWGLALAASRAGVAVGWSWYGGSVLPALAGWVALLLIQPAPKL
                     VLLGLGFAAVFIADVRAERAGAAPAWYPALRKPLTIVVLVSLLGALAATWRH"
     gene            complement(1301..2212)
                     /locus_tag="VGB88_11285"
     CDS             complement(1301..2212)
                     /locus_tag="VGB88_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9648074.1"
                     /translation="MSAGTAGALARLRASWQALPANLRGAVWVLGAALALSAMGACVK
                     LLGARLDSLQIAFFRSLFGLLVVVPFIHRQGGAWAVLRTRRLGALFVRAVLGVGAMLA
                     SFYALTRLPLADAVTLAFTQPLFSIPLAAIVLHETIRAPRWIATACGFAGVTIMVGPS
                     GAEYGVAALVGIAGAFLVACVRIMVKQLSATERPLTILVWFGVFSTLLSLAPALAVWQ
                     APSAGEYVLLALVGALAAGAQTLIIWGLRAGEASAVVPFEYSRLLFAAGFGYLIFGDL
                     PGLATLAGAAVIVASSVFLGRAELRAR"
     gene            complement(2209..3144)
                     /locus_tag="VGB88_11290"
     CDS             complement(2209..3144)
                     /locus_tag="VGB88_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9648075.1"
                     /translation="MSSLKAPPGDAPAAALAGTAPGLKAALWMLLACACFASMNGIVR
                     HVAQELPVLVVVFFRSAFGLIAMLPWLAGSGLASLKPGRPRLHAVRVALAVVAMTAWF
                     YGLKHMPLAEATALSFTTPLFISVAAVVLLGEVMRARRWTATAIGFAGAMIILRPGLV
                     EITDATLAVLLAAMLMAASQILVKLLSRVEHANATVFWLVLLMTPTSLVPALAVWQTP
                     DPGQLAWLAALGAIATIGHQSLARSLRLADATAVYPLDFTRLVFAALIGYLAFAEVPD
                     VWTWIGGAVVMASSVYIAHREARLRRRAQPPAPEP"
     gene            3306..3629
                     /locus_tag="VGB88_11295"
     CDS             3306..3629
                     /locus_tag="VGB88_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648076.1"
                     /translation="MAEGSPDDAAKRFRDIEKGMLEAFERWHRFHEDLLKLGDFSNWL
                     SESENQVDLVTFFALDKDSGTSYRQALEHQKMFLSAIQQLLEQIETMFDEYESAKGAG
                     GEGAG"
     gene            3681..4376
                     /locus_tag="VGB88_11300"
     CDS             3681..4376
                     /locus_tag="VGB88_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX9648077.1"
                     /translation="MNKVYADARAALDGLLFDGMTIMAGGFGLCGIPENLIVALRDSG
                     VKRITVISNNCGIDDFGLGLLLQTRQIAKMVSSYVGENKLFEQQYLNGELELEFNPQG
                     TLAERCRAGGAGIPAFYTRTGVGTQVAEGKETRDFAGETYVMETALTADLAIVKAWLG
                     DSHGNLVYRKTARNFNPAMAMAGKVTVAEVEELVELGGLDPDHVHTPGIFVQRLIEGA
                     AYEKRIERLTLRE"
     gene            4381..5034
                     /locus_tag="VGB88_11305"
     CDS             4381..5034
                     /locus_tag="VGB88_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEX9648078.1"
                     /translation="MAWSREDMCRRAAGELKDGFYVNLGIGMPTMVANYVPDGMHVIL
                     QSENGMLGMGPFPRANEVDADLINAGKQTISELPESVYFDSAASFAMIRGGHIDLSIL
                     GALQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVLMDHNAKDGTPKLLRRCNL
                     PLTGAGVVDMVITDLGVFEIGDGRMRLVEIAPGISLDEIEARTEAGFEVVPELPAPA"
     gene            5031..5531
                     /locus_tag="VGB88_11310"
     CDS             5031..5531
                     /locus_tag="VGB88_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9648079.1"
                     /translation="MSEPSVTTFARYNAWANARLYDACAALPEAAYFAQRACAFFTSL
                     HGTLNHILVGDRAWMRRLTGEGPAPERLDQELYADLASLRVARAAEDARILAFVERLD
                     DAALARTLIYRTMAGETHANPVGLLLAHMFNHQTHHRGQAHALLKDAGAEPPPLDLIY
                     FLRARA"
     gene            5540..6214
                     /locus_tag="VGB88_11315"
     CDS             5540..6214
                     /locus_tag="VGB88_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648080.1"
                     /translation="MAAGARAVLSWSSGKDSAWMLHRLRQAGEVEIVGLLTTVTETYA
                     RVSMHAVRDGLLAAQAEAARLPLHRVPIPDPCPNEVYERAMSRAIERIKADGATAIAF
                     GDLFLADVRRYREQRLAGTGLTPLFPLWGEDTGALARKMVAGGLAATITCVDPKALDA
                     GFCGRAFDASFLDDLPAGVDPCGENGEFHSFAHAGPMFARPIPIRVGETVARGGFVFT
                     DLLPAG"
     gene            complement(6221..6622)
                     /locus_tag="VGB88_11320"
     CDS             complement(6221..6622)
                     /locus_tag="VGB88_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9648081.1"
                     /translation="MSARPFLDTNVLVYAFAANDPRSDRAEALLAAGGVIGVQVLNEF
                     VNVSRRKLGRGWAEIESEVAVVRALLDPPVPLTIDVHEAAVALARNHGFAFYDALVIA
                     AARRAGCTVLYTEDMHHGQAVGELTIQNPFA"
     gene            complement(6612..6842)
                     /locus_tag="VGB88_11325"
     CDS             complement(6612..6842)
                     /locus_tag="VGB88_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9648082.1"
                     /translation="MHVAKWGNSLAVRLPKAVVEALDLKAGDEIEVVVAGGRRFEIVR
                     DRSREQAIARLKSLKRPLPKGFRFDRDEAHER"
     gene            complement(6905..>8167)
                     /locus_tag="VGB88_11330"
     CDS             complement(6905..>8167)
                     /locus_tag="VGB88_11330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9648083.1"
                     /translation="RMTRTRQAQHTDARVRAQYEDYPYPARKPEDEARRLVTGSPSHL
                     AEIIHYLYAGRAPYGPGKRFRALVAGGGTGDGVIMLAQQLATAGADAEVVHLDVARAT
                     ARIAKARARARGLRNIRFVDGSLLDLEGLGLGGFDYIDCCGVLHHLADPAAGLRALVR
                     ALGPGGGIGLMVYGTLGRTGVYHVQDALKLLGEADDPPAARVKQARALLADLPETNWL
                     RRNPFVADHLHGDDAGLHDLLLHARDRAYTVPEVFALCAGAGLAITSFIEAARYEPAT
                     YLHDADLVARAGALAWPERAALAELLSGAIKRHVVYAVAGSAEGRVAAPDSPDMVPVL
                     REGDGQAYARALAAAGRLTAQLENLTLSFSFGPLAPAILSRIDGARSLADIHAALTTD
                     TDWATFKAAFDDLYAKLNGINLLLLRAR"
BASE COUNT         1253 a         2913 c         2902 g         1099 t
ORIGIN      
        1 catcacgctc taggtcccac gctcggcggt caccgctcag ggctcgccct gaagctcgtc
       61 catctcctcg tcggagtagc cgaagtggtg gccgatctcg tggatcagca cgtagcggac
      121 cacctcggcc aagtcggcgc cggtctcgca ccagtagtcg agcagcggcc ggcggtaaag
      181 gtagaccatg tccggctcgg gcgccggctg gctgacgctg cgctcggtca tggccacgcc
      241 ctggtacagc cccatcaggt cgaacgggct gtcgaggccc atgtcgtcga gcacctcgtc
      301 gtccgcgaag tcggtgacct ggacgacgac gttgcccgcc tggcgcctga gcatctcggg
      361 aatggtggcg agcaccgccg ccgcgatctc gcgcagctcg tcctcgctcg gcgggtgtct
      421 cggccggcgc gccatggcgt gcatcatcgc cggcgcgggg cggcggtcaa gcgagccctt
      481 gacggcgccg gcggcggcgc caccctgggc ggcgcgcaac cggaaggagg ccaaggccat
      541 gtccgaaaag atcaccggcg cggcactcga tgcggcgctc gccgggctcg ccggctgggc
      601 ccgcgtcgcg gggcgcgagg cgatttccaa gagctaccgc ttcgccgact tcaatgccgc
      661 gttcggctgg atgacgcgcg ccgcgctgat ggccgagcgc atggaccacc atccggagtg
      721 gttcaacgtc tacaaccgag tcgaggtgac cttggcaagc cacgacgtgg gcggggtcag
      781 cgcgcgcgac atcgcgctcg cccgcttcct cgattcgctg ccggggtgag cgccgggtga
      841 tggcaggcac gcgcgcgagc gtgccgcggc cggcgctggt actgggcgta gccggcgcgg
      901 cgccgttcgt cgccggcgcg ctcgccatgc tggtcgggcc gggctggctc aagctcaccg
      961 cctacgtcta cctggtcaac tacgccgcgg tcgcgctcgc cttcgtcggc gcggtccatt
     1021 gggggctggc gctggcggcg tcgcgcgcgg gcgtcgcggt cggctggtcg tggtacggcg
     1081 gctcggtgct gccggcgctc gccggctggg tcgcgctctt gctgatccag ccggcgccga
     1141 agctggtgct gctcggcttg ggcttcgccg cggtgttcat cgccgacgtg cgcgccgagc
     1201 gcgccggcgc cgcgcccgcc tggtacccgg cgctgcgcaa gccgctgacc atcgtggtgc
     1261 tggtatcgct gctcggggcg ctggcggcga cctggcggca ttagcgtgcg cgcagctcgg
     1321 cgcggccgag gaacaccgag ctcgccacga tcaccgccgc gcctgcgagc gtcgccaggc
     1381 ccggcagatc gccgaagatc aggtagccga agccggccgc gaacaacagc cgcgaatact
     1441 cgaacggcac caccgcgctc gcctcgcccg cgcgcagccc ccagatgatc agggtctggg
     1501 cgccggcggc gagcgcgccg accagcgcca gcagcacgta ctcgcccgcg ctcggcgcct
     1561 gccacacggc gagcgccggt gccagcgaca agagcgtcga gaacacgccg aaccagacca
     1621 ggatggtcag cggccgctcg gtcgcggaca gctgcttgac catgatccgg acgcacgcga
     1681 ccaggaacgc gccggcgatg ccgaccagcg cggcgacgcc gtattcggcg ccgctcggcc
     1741 cgaccatgat cgtcacgccg gcgaagccgc aggcggtggc gatccagcgc ggcgcgcgga
     1801 tcgtctcgtg cagcacgatc gcggccagcg ggatgctgaa cagcggctgg gtgaaggcga
     1861 gcgtgaccgc atccgcgagc ggcagtcggg tgagcgcata gaagctcgcc agcatggcgc
     1921 cgacaccgag cacggcgcgc acgaacaagg cgcccagccg acgcgtgcgc agcaccgccc
     1981 aggcgccgcc ctggcggtgg atgaacggca ccaccaccag cagcccgaac aggctgcgga
     2041 agaacgcgat ctgcaggctg tcgaggcggg cgccgagcag cttgacgcac gcgcccatcg
     2101 ccgacagcgc cagcgccgcg cccagcaccc ataccgcgcc gcgcaggttc gccggcagcg
     2161 cctgccacga tgcgcgcagg cgcgcgagcg cgcccgccgt ccctgcgctc acggctcggg
     2221 cgcgggcggc tgcgcccggc ggcgaagccg ggcctcgcgg tgggcgatat agaccgagct
     2281 cgccatgacc accgcgccgc cgatccaggt ccacacatcg ggcacctcgg cgaaggcgag
     2341 gtagccgatc agcgccgcga acaccaggcg cgtgaagtcg agcggataga ccgcggtcgc
     2401 atccgccagg cgcaggctgc gcgcgagcga ctggtggccg atggtggcga tcgcgccgag
     2461 cgcggcgagc caggcgagct ggccggggtc cggggtctgc cagaccgcga gcgccggcac
     2521 cagcgaggtc ggcgtcatca gcagcaccag ccagaacacg gtggcattcg cgtgctcgac
     2581 ccgcgacaac agcttgacca ggatctggct cgccgccatc agcatcgccg ccagcagcac
     2641 cgcgagcgtg gcgtcggtga tctcgaccag ccccgggcgc aggatgatca tcgcgccggc
     2701 gaagccgatc gcggtcgcgg tccagcgccg tgcccgcatc acctcgccga gcagcaccac
     2761 cgccgccacc gagatgaaca gcggcgtggt aaagctcaac gcggtcgcct cggcgagcgg
     2821 catgtgcttg aggccgtaga accacgcggt catggcgacc accgcgagcg ccacgcgcac
     2881 tgcgtgcaga cgcggccggc ccggcttgag cgaggcgagc cccgagcccg cgagccacgg
     2941 cagcatcgcg atcagcccga aggccgagcg gaagaacacc accaccagca ccggcagctc
     3001 ctgggcgacg tggcggacga tgccgttcat cgacgcgaag caggcgcagg cgagcagcat
     3061 ccacagcgcc gccttcaggc ccggggccgt cccggccaac gccgccgccg gcgcgtctcc
     3121 cggtggcgcc ttcaagctgc tcatcgctgc accctatcgc cggccttgcc cgcccgccag
     3181 cggcgaggcg cgcgggacag gcgcgcgcgc ggcgcagggg ttcgattgac acgcccccgc
     3241 tgcgccgact atgaaccgca agcgtgggcg cccgaccggc gcccggcagg aacaggagaa
     3301 ggcgcgtggc cgagggctcg ccggacgacg ccgccaagcg gttccgcgac atcgagaagg
     3361 gcatgctcga ggcgttcgag cgctggcacc gcttccacga ggacctgctc aagctcggcg
     3421 acttctcgaa ctggctgagc gagtccgaga atcaggtcga cctggtcacc ttcttcgccc
     3481 tcgacaagga cagcggcacc agctaccgcc aggcgctcga gcaccagaag atgttcctgt
     3541 cggcgataca gcagctgctg gagcagatcg agaccatgtt cgacgagtac gagagcgcca
     3601 agggtgcggg cggcgagggc gcgggctgag gcggcgtcga cgacgcaggc cgcgccgctt
     3661 cgacaccggc ggcttcgacg atgaacaagg tctacgccga cgcccgcgcc gcgctcgacg
     3721 gcctgctgtt cgacggcatg acgatcatgg ccggcggctt cggcctgtgc gggattcctg
     3781 agaacctgat cgtggcgctg agggactcgg gcgtgaagcg gatcaccgtg atcagcaaca
     3841 attgcggcat cgacgatttc ggcctcgggc tgttgctgca aacgcgccag atcgccaaga
     3901 tggtgtcgtc ctatgtcggc gagaacaagc tgttcgagca gcaatacctg aacggcgagc
     3961 tcgagctcga gttcaacccg caagggacgc tcgccgagcg ctgccgcgcg ggcggcgccg
     4021 gcatcccggc gttctatacc cgcaccgggg tcggcaccca ggtcgccgag ggcaaggaga
     4081 cgcgcgactt cgccggcgag acctatgtga tggagaccgc gctcaccgcc gacctcgcca
     4141 tcgtcaaggc gtggctcggc gacagtcacg gcaacctcgt ctaccgcaag accgcgcgca
     4201 acttcaaccc ggcgatggcg atggcgggca aggtgaccgt ggccgaggtc gaggagctgg
     4261 tcgagctcgg cgggctcgac cccgaccacg tccatacccc gggaatcttc gtccagcggc
     4321 tgatcgaggg cgcggcctac gagaagcgca tcgagcgctt gacgttgcgg gagtagcgca
     4381 atggcctggt cacgcgagga catgtgccgg cgggcggcgg gcgagctcaa ggacggcttc
     4441 tacgtcaacc tcggcatcgg catgccgacc atggtcgcga actacgtgcc ggacggcatg
     4501 cacgtgatcc tgcaaagcga gaacggcatg ctcggcatgg gcccgttccc gcgcgccaac
     4561 gaggtcgacg ccgacctgat caacgccggc aagcagacca tctcggagct gccggaatcg
     4621 gtctacttcg acagcgccgc ctcgttcgcg atgatccgcg gcggccacat cgacctgtcg
     4681 atcctgggcg cgctgcaggt ggccgagaac ggcgacctcg ccaactggat gatcccgggc
     4741 aagatggtca agggcatggg cggcgccatg gacctggtgg caggggtcaa gcgcgtggtg
     4801 gtgctgatgg accacaacgc caaggacggc acgcccaagc tgctcaggcg ctgcaacctg
     4861 ccgctcaccg gcgcgggcgt ggtcgacatg gtgatcaccg acctcggcgt gttcgagatc
     4921 ggggacggcc gcatgcgcct ggtcgagatc gcgcccggca tctcgctcga cgagatcgag
     4981 gcgcgcaccg aggccgggtt cgaggtcgtg cccgagctgc cggcgccggc gtgagcgagc
     5041 cttccgtcac caccttcgcc cgctacaacg cctgggcgaa tgcgcgcctg tacgacgcct
     5101 gcgcggcctt gccggaggcg gcgtacttcg ctcaacgcgc gtgcgcgttc ttcaccagcc
     5161 tacacggcac gctcaaccac atcctggtcg gcgaccgggc gtggatgcgg cgcttgaccg
     5221 gcgaggggcc ggcgcccgaa cggctggacc aggagctgta tgccgacctc gcctcgttgc
     5281 gcgtcgcgcg cgcggcggag gacgcgcgca tcctcgcctt cgtcgagcgg ctcgacgatg
     5341 ccgcgctcgc gcgcacgctg atctaccgga ccatggccgg cgagacccac gccaacccgg
     5401 tcggcctcct cctcgcgcac atgttcaacc accagaccca ccaccgcggc caggcgcacg
     5461 cgttgctcaa ggacgccggc gccgagccgc cgccgctcga cctgatctac ttcctgcgcg
     5521 cgcgcgcctg aaggttcgga tggcggccgg cgcgcgcgcg gtgctgtcgt ggagcagcgg
     5581 taaggacagc gcctggatgc tgcaccggct gcgccaggcg ggcgaggtcg agatcgttgg
     5641 cctgctcacg accgtcaccg agacctacgc acgcgtgtcg atgcacgcgg tgcgcgacgg
     5701 cctgctcgcg gcgcaggccg aggcggcgcg gctgccgctc catcgggtgc cgattcccga
     5761 cccgtgcccg aacgaggtct atgagcgggc gatgagccgg gcgattgaac ggatcaaggc
     5821 cgacggcgcc accgcgatcg cattcggcga cctgttcctc gccgacgtcc gccgctaccg
     5881 cgaacaacgg ctcgccggca ccgggctcac gcccctgttc ccgctgtggg gcgaggacac
     5941 cggcgcgctc gcccgcaaga tggtcgcggg cgggctcgcc gcgaccatca cctgcgtcga
     6001 tccgaaggcg ctcgatgcgg gcttctgcgg gcgcgccttc gacgcctcgt tcctcgacga
     6061 cctgcccgcc ggcgtcgacc cgtgcggcga aaacggcgag ttccacagct tcgcccatgc
     6121 cgggccgatg ttcgcgcgcc cgatcccgat ccgcgtcggc gagacggtcg cgcgcggcgg
     6181 cttcgtgttc accgacctgc tgcctgccgg ctgatcgctg tcacgcgaac ggattctgga
     6241 tcgtcagctc gccgacggcc tgtccgtggt gcatgtcctc ggtataaaga accgtacagc
     6301 cggcccgtct tgccgcggcg atgaccagag catcgtagaa tgcgaagccg tgattgcggg
     6361 caagcgcgac cgccgcctcg tgcacgtcga tggtcaaggg gacgggcgga tcgagcagcg
     6421 cccgcaccac ggcgacctcg ctttcgatct ccgcccagcc ccggccgagc ttgcgccgcg
     6481 acacgttgac gaactcgttc agcacctgga cgccgatcac gccgcccgcc gcaagcaagg
     6541 cctcggcccg gtcgctgcgc ggatcgttgg cggcaaaggc gtaaacgagc acgttcgtgt
     6601 cgagaaacgg cctagcgctc atgcgcctcg tcccggtcga acctgaatcc cttgggcagg
     6661 ggccgcttga gcgacttcag gcgggcgatg gcctgctcgc ggctgcggtc gcggacaatc
     6721 tcgaaacgtc ggccgccggc gaccacgacc tcgatctcgt cgcccgcctt caggtcgagt
     6781 gcctcgacca cggccttggg caggcgcacg gcgaggctgt tgccccattt ggcgacgtgc
     6841 atggcgacct ctctgatata caattaccat gtatatcata tacgctgact cggcaacctg
     6901 caagtcaccg cgcgcgcagc agcagcaggt tgatgccgtt gagcttggcg taaaggtcat
     6961 cgaacgcggc cttgaacgtg gcccaatcgg tgtcggtggt cagcgcggcg tggatgtcgg
     7021 cgaggctgcg cgcgccgtcg atgcgcgaca ggatcgcggg cgcgagcggg ccgaaggaga
     7081 agctcagcgt caggttctcg agttgcgcgg tgagccgccc tgccgccgcc agcgcgcggg
     7141 cgtaggcctg gccgtcgccc tcgcgcagca caggcaccat gtcgggcgag tcgggcgcgg
     7201 cgacgcggcc ctcggcgctg cccgcgaccg cgtagaccac atggcgcttg atcgcccccg
     7261 acagcagctc ggcgagcgcc gcgcgctcgg gccaggcgag cgcgcccgcg cgcgcgacca
     7321 ggtccgcatc gtgcaggtag gtcgccggct cgtagcgcgc ggcctcgatg aacgaggtga
     7381 tggcgagccc cgcgcctgcg cacagcgcaa acacctcggg cacggtgtaa gcgcggtcgc
     7441 gcgcgtgcag cagcaagtcg tggagcccgg catcgtcgcc atgcaggtga tcggcgacga
     7501 acgggttgcg cctgagccag ttggtctcgg gcagatccgc gagcagcgcg cgcgcctgct
     7561 taacacgcgc ggccggcggg tcgtccgcct cgccgagcag cttgagcgca tcctgcacat
     7621 ggtagacgcc ggtccgcccg agcgtgccgt agaccatcag cccgatcccg ccgccggggc
     7681 cgagcgcgcg caccagcgcg cgcaagcctg cggccggatc ggcgagatgg tgcaggacgc
     7741 cgcagcagtc gatgtagtcg aagccgccga ggccgagccc ctcgaggtcg agcagcgagc
     7801 cgtctacgaa cctgatgttc ctgagcccgc gcgcccgtgc ccgcgccttg gcgatgcgtg
     7861 cggtcgcgcg cgcgacgtcg agatgcacca cctcggcgtc ggcgccggcg gtggcgagct
     7921 gctgggcgag catgatcacc ccgtcgccgg tgccgccgcc cgcgaccagc gcgcggaagc
     7981 gcttgcccgg cccgtagggc gcgcgcccgg cgtacaggta gtggatgatc tcggcgaggt
     8041 ggctcggcga gccggtgacc aggcgccgcg cctcgtcctc gggcttgcgc gccgggtagg
     8101 ggtagtcctc gtactgcgcg cgcacccgcg cgtcggtgtg ttgcgcctgc cgcgtcctgg
     8161 tcatgcg
//
LOCUS       DASWHU010000305         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3487425, whole genome shotgun sequence.
ACCESSION   DASWHU010000305 DASWHU010000000
VERSION     DASWHU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487425"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="gcvPA"
                     /locus_tag="VGB88_11335"
     CDS             complement(<1..1163)
                     /gene="gcvPA"
                     /locus_tag="VGB88_11335"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX9648084.1"
                     /translation="MRYLPLTPADRAAMLETIAASPIGARTIDDLYRDVPAAARLDGL
                     LDLPAHAGEIEVGRALAAFAARNLATSAAPAFLGAGAYRHHVPAAVDALIQRGEFLTS
                     YTPYQPEISQGTLQALFEFQTQVALITGMEVANASMYDGASACAEAVQMASRITRRGR
                     AVLSGGLHPHYAEAVETFARFSGREIVRNRALAPTRRPHDTSALEAAIDAECACVVVQ
                     QPDLFGVVRDFSQLAEACHGAGALLVACVAEPLSLGLIEPPGAWGADIVVGEGQGLGP
                     GLQFGGPPLGLFATRERFVRQMPGRIAGETVDVDGRRGWVLTLSAREQHIRRDKATSN
                     ICTNSGLCALAFSIHLALLGDDGFARLARLNHAYAVRAADRLDEVPGVEVLNET"
     gene            complement(1167..1544)
                     /gene="gcvH"
                     /locus_tag="VGB88_11340"
     CDS             complement(1167..1544)
                     /gene="gcvH"
                     /locus_tag="VGB88_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9648085.1"
                     /translation="MAARRYTEEHEWITVDGDVGTVGISDYAQEQLGDIVYIELPEAG
                     RKLAQGDEAAVIESVKAASELYAPVDGEVVEVNRRLADEPGLVNADATGDGWFVKLRI
                     TDASQLDGLMDDAGYQEFVSNLE"
BASE COUNT          222 a          587 c          603 g          234 t
ORIGIN      
        1 gtctcgttga gcacctcgac gcccggcacc tcgtcaagcc ggtcggcggc gcgcaccgcg
       61 taggcgtggt tgagccgggc gaggcgggca aagccgtcgt cgcccagcag cgccaggtgg
      121 atcgagaagg cgagcgcgca caggcccgaa ttggtgcaga tgttgctggt cgccttgtcg
      181 cggcggatgt gctgctcgcg ggcgctcaag gtcagcaccc agccgcgccg gccgtcgacg
      241 tcaacggtct cgccggcgat ccggcccggc atctggcgca cgaaacgctc gcgggtcgcg
      301 aacaggccga gcggcgggcc gccgaactgg aggcccggcc caagcccctg gccctcgccg
      361 accacgatgt cggcgcccca ggcgccgggc ggctcgatca ggccgagcga cagcggctcg
      421 gcgacgcacg cgaccagcag cgcgccggcg ccgtggcacg cctcggcgag ctggctgaag
      481 tcgcgcacga cgccgaacag gtcgggctgc tggacgacca cgcaggcgca ctcggcgtcg
      541 atcgcggcct cgagcgcgct ggtatcatgc ggccggcgcg tgggcgcgag cgcccggttg
      601 cgcacgatct cgcgccccga gaaccgggcg aacgtctcga ccgcctcggc atagtgcggg
      661 tgcaagccgc ccgacagcac cgcccggccg cggcgggtga tccggctcgc catctgcacc
      721 gcttcggcgc aggccgaggc gccgtcatac atcgaggcgt tggccacctc catgccggtg
      781 atcagcgcga cctgggtctg gaactcgaac agcgcctgca gggtgccctg gctgatctcg
      841 ggctggtagg gggtgtacga ggtcaggaac tcgccgcgct ggatcagcgc atcgaccgcg
      901 gccggcacgt ggtggcggta ggcgccggcg ccgagaaagg cgggcgcggc gctggtcgcg
      961 aggttcctgg cggcaaacgc ggccaatgcg cggccgacct cgatctcgcc ggcatgcgcc
     1021 ggcaggtcga gcaggccatc gaggcgcgcg gctgcgggca cgtcgcggta cagatcgtcg
     1081 atggtgcgcg cgccgatcgg gctcgcggca atggtctcga gcatcgccgc gcggtcggcc
     1141 ggggtcagcg gcaggtagcg catggcctac tccaggttgc tcacgaactc ctgatagccg
     1201 gcgtcgtcca tcagcccgtc gagctggctc gcgtcggtga tcctgagctt gacgaaccag
     1261 ccgtcgccgg tggcgtcggc attgaccagg ccgggctcgt cggcgaggcg gcggttgacc
     1321 tcgaccacct cgccgtcgac cggggcgtac agctcgctcg ccgccttgac cgactcgatc
     1381 accgccgcct cgtcgccctg cgcgagcttg cgcccggcct cgggcagctc gatgtagacg
     1441 atgtcgccga gctgctcctg ggcgtagtcg ctgatgccga cggtgccgac gtcgccgtcg
     1501 acggtgatcc actcgtgctc ctcggtatat ctgcgtgccg ccatcgatcg tctccctccc
     1561 ttgcgcgatt gccccggggc ttgtggtcag cccttgcggt aacggtgcgg cacgaacggc
     1621 agcgccgcca cctcggccgg cacctg
//
LOCUS       DASWHU010000306         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3148062, whole genome shotgun sequence.
ACCESSION   DASWHU010000306 DASWHU010000000
VERSION     DASWHU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148062"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..942)
                     /locus_tag="VGB88_11345"
     CDS             complement(55..942)
                     /locus_tag="VGB88_11345"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9648086.1"
                     /translation="MNATVDCVLDCRCTLGEGLVWDEQAGRLWWTDIPAGRIYALERG
                     SGAVRTFDLPGPVGSFGLCAGSDSLVVATVGGVHLFEPATGRLELIVDPEPEMPTNRL
                     NDGKIGPDGAFWVGSMDNRPLAEKQPVGALYRVTAGGGCERKVAGVIVSNGIGWTGDG
                     RTMYHSDSRGMWIDRWDCDTATGEITNRVRIAEPGPADGRPDGAAVDIEDCYWSAGVS
                     AGCLNRYAPDGELLTKIEVPVPTPTMCCFGDGDMQTLFITSLQVDVPADVLARHPWTG
                     SVLALRLPIAGVPTWRFKL"
     gene            complement(951..1913)
                     /locus_tag="VGB88_11350"
     CDS             complement(951..1913)
                     /locus_tag="VGB88_11350"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEX9648087.1"
                     /translation="MPYYVLVDDEQTGPFEDAAIADMVARGEVTADTLVWAAGMADWA
                     PARGVAGLASLFGAAPPPAAGFARRPAAPVPAAAGPVERLDIAAAIATGLGAFSRQPG
                     RAMLAALVYNIVPIVLVAPVFGVLFALYGGEPEAGEANPGMMVLIALLVVAMLAVVVI
                     LYGGFCACMIDLVRDRQVEVAKVFSGFRRAGPLIAFGILYALAVAVGFMVLIAPGVFV
                     AVAFMLTPYIVIESELSGTAAMKASFGAIMTLGWWRCFALLVLLFAALVVASLVLEGL
                     GLVLGDLVHALLQFAFNIALTAVAGAIFAAIYEQARRNSERAEA"
     gene            2264..>2840
                     /locus_tag="VGB88_11355"
     CDS             2264..>2840
                     /locus_tag="VGB88_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648088.1"
                     /translation="MALLFASPVDDPVAWTVALTRLEPTLEVRVWPAAGALDAIEAAL
                     VWRPPAGLLASLPNLKLIQSLGAGIDHILADPALPDGVPICRLVDPALTRQMVEYVTL
                     AVLHHHRRMADYREFQARAEWHQLAPLEGRHRRVGVMGLGVIGAAVAQRLAEFDFTVA
                     GWCRTETTIPNVASFYGPGSLAPFLARSDVLV"
BASE COUNT          422 a         1048 c          994 g          376 t
ORIGIN      
        1 ttcggtcaag aagattagat tggcaatgta gcgctcatcg gccggagcgg tagctcacag
       61 cttgaagcgc caggtgggga cgccggcgat tgggaggcgc agcgccagca cgctgccggt
      121 ccaggggtgg cgcgcgagca cgtccgcggg cacgtccacc tgcaggctgg tgatgaacaa
      181 ggtctgcatg tccccgtcgc cgaagcagca catggtcggc gtcggcaccg gcacctcgat
      241 cttggtcaag agctcgccgt cgggcgcgta gcggttgagg cagccggcgg agacgccggc
      301 gctccagtag cagtcctcga tgtcgaccgc ggcgccgtcc gggcggccgt ccgcaggccc
      361 gggctctgcg atgcgcaccc ggttcgtgat ctcgccggtc gccgtatcgc agtcccagcg
      421 gtcgatccac atgccgcggc tgtcgctgtg gtacatggtg cgcccgtcgc cggtccagcc
      481 gatgccgttc gacacgatca cgccggcgac cttccgctcg cagccgccgc ccgcggtgac
      541 ccgatagagc gcgccgacgg gctgcttctc ggccagcggg cggttgtcca tgctgcccac
      601 ccagaacgcg ccgtcggggc cgatcttgcc gtcgttcagc cggttggtcg gcatttccgg
      661 ttcggggtcg acgatcagct cgaggcgccc ggtcgcgggc tcgaacaggt gcacgccgcc
      721 gacggtcgcc acgaccagcg agtcgctccc cgcgcacagc ccgaacgagc cgaccgggcc
      781 cggcaggtcg aaggtccgca ccgcgcccga gccgcgctcg agggcgtaga tgcgccccgc
      841 cgggatgtcg gtccaccaca gccgcccggc ctgctcgtcc cagaccaagc cctcgccgag
      901 ggtgcagcgg cagtcgagca cgcagtcgac ggtcgcgttc atggcgcgcc tcaggcctcc
      961 gcgcgttcgg aattgcgccg cgcctgctcg tagatcgcgg cgaagatggc gccggcgacc
     1021 gcggtcagcg cgatgttgaa cgcgaactga agcagcgcgt ggaccaggtc gccgagcacc
     1081 aggccgagcc cctccagcac cagcgacgcg accaccaggg cggcgaacag cagcaccagg
     1141 agcgcgaagc agcgccacca gcccagcgtc atgatcgcgc cgaagctcgc cttcatcgcc
     1201 gcggtcccgg acagctcgct ctcgataacg atgtagggcg tcagcatgaa cgccaccgcg
     1261 acgaacacgc cgggcgcgat cagcaccatg aacccgaccg cgaccgcgag cgcgtacagg
     1321 atgccgaagg cgatcagcgg gccggcgcgg cggaagcccg agaacacctt ggcgacctcc
     1381 acctgccggt cgcggaccag gtcgatcatg caggcgcaga agccgccgta gaggatcacc
     1441 accacggcca gcatcgccac caccagcagc gcgatcagca ccatcatgcc cgggttcgcc
     1501 tcgcccgctt cgggctcgcc cccatagagc gcgaacagga cgccgaacac cggcgcgacc
     1561 agcacgatcg gcacgatatt gtagaccagc gccgccagca tcgcgcggcc cggctggcgg
     1621 gagaacgcgc cgagaccggt cgcgatcgcg gcggcgatgt cgagcctctc gacagggccc
     1681 gcggctgccg gcacgggcgc ggccggcctg cgtgcgaacc cggccgccgg cggcggcgcc
     1741 gcgccgaaca gggacgcgag cccggcgacg ccgcgcgcgg gcgcccagtc ggccatgccc
     1801 gcggcccaca ccagggtgtc cgccgtcacc tcgccgcggg cgaccatgtc ggcgatggcc
     1861 gcgtcctcga acgggcccgt ctgctcgtcg tcgaccagga cgtaatacgg cattgccccc
     1921 actccggcgc cgcgcgcgcc gggcgagcct acaccagtgc gcgcgcctct gcgatcccgc
     1981 ttccgcgctc gagtcatatg catccgatga tcgagcgacg ggctgggtgg gtgttgggca
     2041 cgcggagtcg cgcagacgcg gcggatcggt aagcgaactg ggcgattgcc gtcgctgcgc
     2101 ccgccttgtg ctccgcggcc tccgcgcctc cgcgtgaacc gaatcacccg tggccgaatg
     2161 cgcacgcgaa acggtgcatg ggcgatgacc tcgctgcgcc gccggccttg tctcgaccgc
     2221 cgccattggc gataacggcg cgcgcgattg gggagaaggg gcgatggcgt tgctgtttgc
     2281 gagcccggtc gacgatccgg tggcgtggac cgtcgcgctc acccggctcg agccgacgct
     2341 cgaggtgcgc gtgtggcccg ctgccggcgc gctcgatgcg atcgaggcgg cgctggtctg
     2401 gcgccccccc gccggcctgc tcgcaagcct gccgaacctc aagctgatcc agtcgctcgg
     2461 cgccggcatc gaccacatcc tcgccgatcc cgcgctgccc gacggcgtgc cgatatgccg
     2521 gctggtcgat cccgcgctga cccgccagat ggtcgagtac gtgacccttg ccgtgctcca
     2581 ccaccaccgg cgcatggccg actaccggga gttccaggcg cgcgccgagt ggcaccagct
     2641 ggcgccgctc gagggccggc accggcgcgt cggcgtgatg ggcctcggcg tgatcggcgc
     2701 ggcggtggcg caaaggctcg ccgagttcga cttcacggtc gcgggctggt gccggaccga
     2761 gacgacgatc cccaacgtgg cgagcttcta tgggccgggc agcctcgcgc cgttcctcgc
     2821 ccgcagcgac gtgctggtct
//
LOCUS       DASWHU010000307         6752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_170569, whole genome shotgun sequence.
ACCESSION   DASWHU010000307 DASWHU010000000
VERSION     DASWHU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170569"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGB88_11360"
     CDS             <1..426
                     /locus_tag="VGB88_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX9648089.1"
                     /translation="PAVISNLYGAGFPTEFEWTGTRDYSAVLAVPAALAFWRELGPAA
                     ARDHMASLAAEAAATLSAAWDSEAAAPPAMCAAMAAVRAPVAGAADYAAARALRDRVL
                     AEHRIEVAVNPIDDSLWVRVSAHVYNSPDQYLRLAEALR"
     gene            complement(428..1183)
                     /gene="ubiG"
                     /locus_tag="VGB88_11365"
     CDS             complement(428..1183)
                     /gene="ubiG"
                     /locus_tag="VGB88_11365"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX9648090.1"
                     /translation="MEQPTADDRTADADEVARFAQLAERWWDARGPLAPLHRLNPTRI
                     RYIRDTVAARLGRDPERPRPLDGLTLLDIGCGGGLIAEPMVRLGARVTGIDAAAANIA
                     AARLHAEAVGLDIDYRSEPVEALAGAGARYDVVLALEVVEHVADLAAFAAAAGACVAP
                     GGAIIVATLNRTLKAYALAVVGAEYLLGWLPRGTHDWSKFVRPSELAGLIADAGLAIE
                     DMTGVCYRPLDGRWALCRDLAVNYMIAAGRPAD"
     gene            1296..2516
                     /locus_tag="VGB88_11370"
     CDS             1296..2516
                     /locus_tag="VGB88_11370"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9648091.1"
                     /translation="MARIVQKFGGTSVADLDRIRSAAGRVKRAHDAGDRIAVVVSAMA
                     GATNQLDLWTRSIARLHDAREYDVVVSSGEQVSVGLMALALQDIGVAARSWLGWQVPI
                     RTDGMHGRARITRIDAAEIGRRMDSGEVAVVAGFQGLGPRNRVTTLGRGGSDATAVAL
                     ASALGAERCDIFTDVDGVYTSDPRIVAKARKLDRISYEEMLEMASLGAKVLQTRAVEI
                     AMRHRVTVQVLSSFSDAPGTLLVDEDEIVEKDIVSGIAYSRDEAKVTLTRIADRPGVA
                     ASIFGPLADSGINVDMIVQASSHDGRFTDLTFTVGRTELARALEVLAGRKADLGYDEL
                     LSDPSVVKVSVIGVGMRTHAGVAQKMFQTLSKRGINIQVISTSEIKISVLIAEEYTEL
                     ALRSLHSAYGLDAE"
     gene            2506..3597
                     /locus_tag="VGB88_11375"
     CDS             2506..3597
                     /locus_tag="VGB88_11375"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9648092.1"
                     /translation="MPNESPVALPHGQVRDPGRLDRLWRRGTALLGTRYAIMGGAMSW
                     VSERHLVAAISNAGGFGVIACAAMGPARLSEEIAATKALTDLPFGVNLITMHPELDDL
                     IEACAEQEVSHVVLAGGLPRAQAIGRIKGSGARLLCFAPALSLARKLVRMGVDALIIE
                     GMEAGGHVGPVATSVLAQEILPEIGEVPVFVAGGIGRGEAIAGYLEMGASGCQLGTRF
                     VCATESIAHPAFKRAFIRASARDAQTSVQLDARFPVIPVRALANAASARFIDCQRQVI
                     ARYQGGELDMKAAQLEIERFWVGALRRAVIDGDVEGGSVMAGQSVGMVTAEQSTADII
                     AELVSEAAVALARRGDAAESEAAAPDPSE"
     gene            3594..5861
                     /gene="ptsP"
                     /locus_tag="VGB88_11380"
     CDS             3594..5861
                     /gene="ptsP"
                     /locus_tag="VGB88_11380"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX9648093.1"
                     /translation="MTQSPSITGPRKLLQRLRDVMAGHGSAQDRLDRIVRIIAADMVA
                     EVCSCYLMRAGEVLELFATQGLNPDAVHKTRLRVNEGLVGYVAGHARPLNLADAQSHP
                     LFAYRPETGEEIYQSLMGVPILRDGRVVGVLVVQNVTRRHYDEEEVEALQVVAMVLAE
                     LVAGGELVDARELFASEDRLSGPATIEGLSLMRGIAMGTVVLKRPRPQLGRLVADDPA
                     EERARLTQAVAALQAGIDRMLDAPEVAEGSASHDVLETYRMFARDSGWLRRIEDAIGS
                     GLTAEAAVIRVQEDNRLRLAHAASGYLRERLLDLEELGDRLLHHLAGDAVGPPQALPD
                     DIVLVARSLGPADLLEYDRAALRAVVLEEGSTTMHLVIVARAFEIPVVGQVRDALSRL
                     APGETVLVDGNAGRVTVRPSDEQVGLFRAGAAARAEQRAYFETLRERKARTRDGIELA
                     LNINVGLSVDADQIAAVGADGVGLCRTEIPFMVHHRFPDVAAQASLYRAVLDVAGERP
                     VMFRTLDIGGDKRLPYLPVIPEENPAMGWRAVRIGLDQPAILRHQLRALVQAAAGRAL
                     NVMFPMVAIVDEFLRARALLSRELDRARALGSAMPKAVRAGVMLEVPALMWQLPALLS
                     AVDFVSVGTNDLVQFLFASDRGNPLLARRYGLLSPATLALLRDLVAACEVSGVALSVC
                     GEAAGEPLEAMAMIGVGVRSLSMPPGRIGPVKAMCLSLAVGPLAHYLENHYQAPDADL
                     GQKLRAFARDHGVSL"
     gene            6030..>6752
                     /locus_tag="VGB88_11385"
     CDS             6030..>6752
                     /locus_tag="VGB88_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9648094.1"
                     /translation="MAQIEPIELAKYREPGVAGYAGVGAEIRDAREAAGRTLAEVADA
                     LRIRPVHLQAIEEGRFDDLPGRVYAIGFLRAYAGFLALDPQLVVSRFRQETAGGAAGP
                     ELNFPEPPPQNWRPNIGLFGLALAIVVGAYAAWYMLRTADDLSLQLVAEVPARLLEAA
                     TPPAPPSVTVAEAPVEAEADAEAAVAAMADVASAPTEEVTPDPLPRAEAAPATARPEP
                     AEDIVEGTGVAAAPPELRLGGAG"
BASE COUNT          904 a         2363 c         2508 g          977 t
ORIGIN      
        1 ccggcggtga tctcgaacct ctacggcgcc ggtttcccga ccgagttcga gtggaccggc
       61 acccgcgact actcggccgt cctggcggtg ccggcggcgc tcgccttctg gcgcgagctc
      121 ggcccggcgg cggcgcgcga ccacatggcc agccttgcgg ccgaggcggc ggcgacgctg
      181 agcgcggcct gggacagcga ggccgcggcg ccgccggcga tgtgcgcggc gatggcggcg
      241 gtacgcgccc cggtcgccgg cgccgccgac tacgccgcgg cgcgcgcgct gcgcgaccgc
      301 gtactcgccg agcaccgcat cgaggtcgcg gtcaacccga tcgacgattc gctctgggtg
      361 cgcgtctcgg cgcatgtcta caacagcccc gaccagtacc tgaggctcgc cgaggcgctc
      421 cgctgagtca gtcggccggc cggccggcgg cgatcatgta gttgaccgcg aggtcgcggc
      481 acaacgccca gcggccgtcg agcgggcggt agcagacccc ggtcatgtcc tcgatcgcga
      541 gcccggcgtc ggcgatcagc ccggcgagct ccgaggggcg cacgaacttc gaccagtcgt
      601 gggtgccgcg cggcagccag ccgagcaggt actcggcgcc gaccaccgca agggcatacg
      661 ccttcagggt gcggttgagt gtggcgacga tgatcgcccc gcccggcgcg acgcaggcgc
      721 cggcggcggc ggcgaaggcg gcgagatcgg cgacgtgctc gaccacctcg agcgccagca
      781 ccacgtcgta gcgcgcgccg gcgccggcca gcgcctcgac cggctcgctg cgatagtcga
      841 tgtcgaggcc gaccgcctcg gcgtgcaggc gcgcggcggc gatgttggcc gcggcggcgt
      901 cgatgccggt gacccgcgcg ccgagcctca ccatcggctc ggcgatcagg ccgccgccgc
      961 agccgatgtc gagcagcgtg agcccgtcga gcggccgcgg ccgttcggga tcgcgcccga
     1021 ggcgcgccgc gaccgtgtcg cggatgtagc ggatgcgggt cgggttgagc cgatgcagag
     1081 gcgccagcgg gccgcgcgcg tcccaccagc gctcggcaag ctgcgcgaac cgggcgacct
     1141 cgtcggcgtc tgcggtgcgg tcgtctgcgg tcggctgttc catcgcctcg atgtcggctt
     1201 gcgagggcgc cggcggaaac gtatgtatag ccgcgcccgc gggcaactgc ggggtgtttt
     1261 ttgctgcccg cagctcgatc gggagccggc gccgcatggc ccgcatcgta cagaaattcg
     1321 gcgggacctc ggtcgcagac ctcgaccgca tccgcagcgc cgccgggcgc gtcaagcgcg
     1381 cccatgacgc cggcgaccgg atcgcggtgg tggtctcggc gatggccggc gccaccaacc
     1441 agctcgacct gtggacgcgc agcatcgccc ggctgcacga tgcgcgcgag tacgacgtgg
     1501 tcgtctcgtc gggcgagcag gtcagcgtcg ggctgatggc gctggcgctg caggacatcg
     1561 gcgtcgcggc gcgctcgtgg ctcggctggc aggtgccgat ccgcaccgac ggcatgcacg
     1621 gccgggcgcg catcacgcgt atcgacgcgg ccgagatcgg ccggcgcatg gactcgggcg
     1681 aggtcgccgt ggtcgccggc ttccaggggc tcgggccgcg caaccgggtc accacgctgg
     1741 gccgcggcgg ctccgacgcg accgcggtgg cgctcgcctc ggcgctcggc gccgaacgct
     1801 gcgacatctt caccgacgtc gacggcgtct acaccagcga cccgcgcatc gttgccaagg
     1861 cccgcaagct tgatagaatt tcctacgagg agatgctcga gatggcgtcg ctcggcgcca
     1921 aggtgctgca gacccgggcg gtcgagatcg ccatgcgtca ccgggtcacc gtccaggtcc
     1981 tgtcaagctt cagcgacgcg cccggaaccc ttctggtcga cgaggacgag atcgtggaaa
     2041 aggacatcgt cagcggcatc gcctacagcc gggacgaggc gaaggtgacg ctcacccgga
     2101 tcgccgaccg ccccggggtg gcggcgagca tcttcggccc gctcgccgac tcaggcatca
     2161 atgtcgacat gatcgtgcag gcctcctcgc acgacggccg cttcaccgac ctcaccttca
     2221 ccgtcgggcg gaccgagctt gcgcgcgcgc tcgaggtgct cgccggccgc aaggcggacc
     2281 tcggctacga cgagctgctg tccgatccct cggtggtcaa ggtgtcggtg atcggggtcg
     2341 gcatgcgcac ccatgccggc gtcgcccaga agatgttcca gaccctgtcc aagcgcggca
     2401 tcaacatcca ggtgatctcg acctccgaga tcaagatcag cgtcctgatc gccgaggaat
     2461 ataccgagct ggcgttgcgc tcgctgcaca gcgcctatgg cctcgatgcc gaatgagtcg
     2521 cccgtggcgt tgccgcatgg gcaagtgcgc gaccctggcc ggctcgaccg gttgtggcgg
     2581 cggggcacgg ccctgctcgg cacccgctac gcgatcatgg gcggcgccat gtcgtgggtg
     2641 tccgagcgcc acctggtcgc cgcgatctcg aatgccggcg gcttcggggt catcgcctgc
     2701 gccgcgatgg ggccggcgcg cctgagtgag gagatcgccg ccaccaaggc gctgaccgac
     2761 ctgccgttcg gggtcaacct gatcaccatg caccccgagc tcgatgacct gatcgaggcg
     2821 tgcgcggagc aggaggtgag ccacgtggtg ctcgccggcg gcctgccgcg cgcccaggcg
     2881 atcgggcgca tcaagggctc gggcgcgcgc ctgctgtgct tcgcgccggc gctgtcgctc
     2941 gcccgcaagc tggtgcgcat gggggtcgat gcgctgatca tcgagggcat ggaggcgggc
     3001 ggccatgtcg gcccggtcgc gaccagcgtg ctggcccagg agatcctgcc cgagatcggc
     3061 gaggtgccgg tgttcgtcgc cggcggcatc ggccgcggcg aggcgatcgc cggctatctc
     3121 gagatgggcg ccagcggctg ccagctcggc acccggttcg tgtgcgcgac cgaatcgatc
     3181 gcccatccgg cgttcaagcg cgcgttcatc cgcgccagcg cccgcgacgc ccagacctcg
     3241 gtccagctcg atgcgcgctt tccggtgatc ccggtgcgcg cgctggccaa tgcggcgagc
     3301 gcccggttca tcgactgcca gcgccaggtg atcgcgcgct accagggggg cgagctcgac
     3361 atgaaggccg cccagctcga gatcgagcgt ttctgggtcg gcgcgctcag gcgggcggtg
     3421 atcgacggcg acgtcgaggg cggctcggtg atggccggcc agagcgtcgg catggtcacg
     3481 gccgagcaat cgaccgccga catcatcgcc gagctggtaa gcgaggccgc ggtggcgctg
     3541 gcgcgccggg gggatgcggc ggagagcgaa gcagcggcgc ccgacccgag cgaatgaccc
     3601 agtcaccgag catcaccggg ccgcgcaagc tgttgcagag gttgcgcgat gtcatggccg
     3661 ggcatggctc ggcgcaggac cggctcgacc gcatcgtgcg catcatcgcc gccgacatgg
     3721 tcgccgaggt gtgctcgtgc tacctgatgc gcgccggcga ggtgctggag ctgttcgcca
     3781 cccagggcct caacccggat gccgtgcaca agacccggct cagggtgaac gagggcctgg
     3841 tcggctacgt cgccggccac gcccgcccgc tcaacctggc cgacgcccag tcgcacccgc
     3901 tgttcgccta ccgcccggag accggcgagg agatctacca gtcgctgatg ggcgtgccga
     3961 tcctgcgcga cgggcgcgtg gtcggcgtgc tggtggtcca gaacgtcacc cggcgccatt
     4021 acgacgagga ggaggtcgag gcgctgcagg tggtggccat ggtgctcgcc gagctggtcg
     4081 ccggcggcga gctggtggat gcgcgcgagc tgttcgcctc ggaggaccgc ctgtccgggc
     4141 ccgcgaccat cgagggcctg tcgctgatgc gcggcatcgc catgggcacc gtggtgctca
     4201 agcggccgcg gccgcagctc ggccggctgg tcgccgacga cccggcggag gagcgggcgc
     4261 ggctcaccca ggcggtggcg gcgctgcagg cgggcatcga ccgcatgctg gatgcgccgg
     4321 aagtagccga gggcagcgcc tcccacgacg tgctcgagac ctatcgcatg ttcgcgcgcg
     4381 acagcggctg gctcaggcgc atcgaggacg ccatcggctc cgggctgacc gccgaggcgg
     4441 cggtgatccg ggtccaggaa gacaaccgac tcaggctcgc gcacgccgcc agcggctatc
     4501 tgcgcgagcg cctgctcgac ctggaggagc tcggcgaccg cctgcttcac cacctcgcgg
     4561 gcgatgcggt cgggccgccg caagcgctgc ccgacgacat cgtgctggtc gcgcgcagcc
     4621 tcggccccgc cgacctcctg gagtacgacc gcgccgcgct gcgcgcggtg gtgctcgagg
     4681 agggctcgac caccatgcac ctggtgatcg tcgcgcgcgc cttcgagatt ccggtggtcg
     4741 gccaggtccg cgacgcgctc agccggctcg cccccggcga gacggtgctg gtcgacggca
     4801 acgccggccg ggtgaccgtg cggccgtcgg acgagcaagt cgggctgttt cgcgccggcg
     4861 ccgccgcccg cgccgagcag cgcgcctatt tcgagacctt gcgcgagcgc aaggcgcgca
     4921 cccgcgacgg tatcgagctg gcgctcaaca tcaatgtcgg gctgtcggtc gatgccgatc
     4981 agatcgcggc ggtgggcgcc gacggcgtcg gcctgtgccg gaccgagatc ccgttcatgg
     5041 tccaccaccg cttccccgac gtcgccgccc aggccagcct ctaccgcgcc gtgctcgacg
     5101 tcgccggcga gcgcccggtg atgttccgca ccctcgacat cggcggcgac aagcgcctgc
     5161 cctaccttcc ggtcattccg gaggaaaatc ccgcaatggg gtggcgtgcc gtgcgcatcg
     5221 ggctggacca gccggccatc ctgcgccacc agctgcgcgc gctggtgcag gcggcggcgg
     5281 ggcgggcgct caacgtgatg ttcccgatgg tggccatcgt cgacgagttc ctgcgtgccc
     5341 gggcgctgct cagccgcgag ctcgaccgcg cccgcgccct cggctcggcg atgccgaagg
     5401 cggtgcgcgc gggcgtcatg ctcgaggtcc cggccctgat gtggcagctg ccggcgctgc
     5461 tcagcgcggt cgacttcgtt tcggtcggca ccaacgacct ggtccagttc ctgttcgcca
     5521 gcgaccgcgg gaatccgttg ctggcccgcc gttacggcct gctgtcgccg gcgacgctgg
     5581 cgctgctgcg cgacctggtc gccgcctgcg aggtgtcggg cgtggcgctg tcggtgtgcg
     5641 gcgaggcggc gggcgagccg ctggaggcga tggccatgat tggggtcggt gtgcgcagcc
     5701 tgtcgatgcc gcccggtcgc atcggcccgg tcaaggcgat gtgcttgagc cttgccgtgg
     5761 ggccgcttgc gcactatctg gaaaatcatt atcaggcccc ggatgccgat ctggggcaaa
     5821 agttgcgcgc gttcgcgcgc gatcacggcg tcagtctgtg agtaaacctt gtcgctccat
     5881 tggttgccga gcgactttaa atttgacgag ccgggagcat atttgtttta tcgggattgc
     5941 aagcaaaacg cccgactccc tggtcgtccc gtgcgtcggt gagaccagag cgcagcgtcc
     6001 tgggggggcc gggtgcaagg ttgacgcgcg tggcgcaaat cgaaccgatt gagctggcca
     6061 agtatcgcga accgggtgtc gcgggctatg ccggagtcgg tgccgagatt cgcgacgcgc
     6121 gcgaggcggc gggccgcacc ctggccgagg tcgccgacgc attgcgtatc cggccggtcc
     6181 atctgcaggc gatcgaggag ggccgattcg atgacctgcc gggtcgggtc tacgcgatcg
     6241 gcttcctcag ggcgtatgcc gggttcctcg ccctcgaccc gcagctggtg gtttcgcgct
     6301 tccgccagga gacggcgggg ggcgcggccg gtcccgagct caacttcccc gagccgccgc
     6361 cgcagaactg gcggcccaac ataggcctgt tcggcctggc gctggcgatc gtcgtcggcg
     6421 cctacgcggc gtggtacatg ctgcggaccg cggacgatct gtcgctgcag ctggtggcgg
     6481 aggtgccggc gcggctgttg gaggcggcga cgccgcccgc gccacccagc gtcaccgtcg
     6541 ccgaggcgcc ggtcgaggcc gaggccgacg ccgaggctgc ggtggcggcg atggcagacg
     6601 tggcatcggc accgaccgag gaggtgacgc ccgatccgct gccgcgggcg gaggctgcgc
     6661 ccgccaccgc ccggccggag cctgccgagg acatcgtcga gggcaccggg gtcgccgcgg
     6721 ccccgcccga gctgcgcctg ggcggcgccg gc
//
LOCUS       DASWHU010000308         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_556789, whole genome shotgun sequence.
ACCESSION   DASWHU010000308 DASWHU010000000
VERSION     DASWHU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556789"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VGB88_11390"
     CDS             <1..441
                     /locus_tag="VGB88_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9648095.1"
                     /translation="AIVVGDPAEHADAFAASRAAITKSGTSTLQLALAGVPMVVCYRV
                     NPLTAFLARRLITVANVALVNLLADEPVVPELLQERCTPQNIADALDALIADEAARSA
                     QRAALAKVAAGLGTAAGAPSELAARIVLDAIEAGAGAARRKAGA"
     gene            438..1172
                     /locus_tag="VGB88_11395"
     CDS             438..1172
                     /locus_tag="VGB88_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648096.1"
                     /translation="MSAVETQVTRGAVTFRAEGISKVYRTGAVAVHALRGVDIAFYAG
                     ELAVLLGPSGSGKSTFLNILGGLDRPTEGRVFFDEHELTALGDAALTEFRRNHVGFVF
                     QFYNLIPSLTARENVALVTEIARRPIRPEDALAMVGLGARLDHFPAELSGGEQQRVAI
                     ARAIAKQPEVLLCDEPTGALDSNTGVVVLEAIDRVNRELGTTTALITHNAAIAGMADR
                     VITFADGRIAGVRDNAPRLAPAQISW"
     gene            1186..3549
                     /locus_tag="VGB88_11400"
     CDS             1186..3549
                     /locus_tag="VGB88_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9648097.1"
                     /translation="MTSLDRKLVRDLWHIRGQALAIALMMAAGVAMSVMSLGMLRSLE
                     ETLGAYYERYRFADVFAQVKRAPDWLATRVAAIPGVGVVETRIVADVTLNVPGLLEPA
                     TGRLISLPERGAPHIDDIAIRQGRRLAPGRASEVVASEAFAEANRLVPGDTFSAVING
                     VERRLTIVGIALSPEYVYSIGPGALVPDDRRFGVLWMGSRTLAAAFDMDGAFNDLALA
                     LLPGASQDEVVARLDQLLDPYGGTGAYAREHQQSNWFLSNELKELTTLATIAPPIFLG
                     VAAFLLNVVASRLVETQREQVGLLKAFGYGNLAVAWHYAKLVGVIVIVGAALGVGAGI
                     WLGRGLAELYTQFFRFPFLHYQLGGQALAVPVLASIAAAALGAFAALRRVFRLAPAVA
                     MQPPAPMRYGRTGAFGLRLVNALSPPSRMIARHIVRRPLRSALTVVSLSSAVAVLIAA
                     MFMLDAIDYMLEVHFHQSQRQDLTVSFTEPEPGRVVHDAARLPGVLRAEPFRAVPVRL
                     RFGHLSERTSIQGVDPAATLGRQLDHALAPLSAPPAGLMLSSWLAGELGVGLGDRITV
                     EVMEGRRPVRALPVTALVEELIGARAYMRMDALNRLMGEGPSVSSVNLQIDPERSADL
                     YRRLKETPAVADTVILTAALQSVRDTLGETLLKMTSFQIAFAAIIAFGVVYNSARISL
                     SERGRELASLRVIGFRHGEVSFILLGELAVLTLLALPLGCVIGRGLAWALAVGLQSDL
                     YRVPLVVERSTYGLSVALVVAAWVACALVVALRVRRLDLIAVLKTRE"
     gene            3556..4779
                     /locus_tag="VGB88_11405"
     CDS             3556..4779
                     /locus_tag="VGB88_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9648098.1"
                     /translation="MSGPARRMLATVAAALVVAAALAWAFWPEPVPVDLAVAARAPMQ
                     VTVDEEGETRVQDIYVVSVPLTGWLRRVQVKAGDAVDGGKTVIATMQETEPSFLDVRS
                     RRQGESRVRAAEAAYALAEAERKRALAELDYARSEWQRAQTLAERGNISTAARDRAEL
                     ALRSQEAALATAEAAVNVRASEVETARAELIAPTVGGFGDEPEGVELGVAVRSPVSGR
                     VLRVIKESAGVVQSGMPLVEVGDPANLEVVVDLLSEDAVKVGAGDPVLIEQWGGQGEL
                     AGRVRRVEPYGFTKVSALGIEEQRVNVVIDFTDAREKWQALGHGFRVEVRIVIWQADD
                     VLQVPIGAMFRIGDAWAVFVDTGGRAARRTIEVGHLNDRTAEVLSGLDEGEAVVLHPS
                     DRVAEGARILKRLVE"
     gene            4835..>5116
                     /locus_tag="VGB88_11410"
     CDS             4835..>5116
                     /locus_tag="VGB88_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9648099.1"
                     /translation="MSGLARWAWVIGVVAAAAAPARAADYDAAIAATAAAIEGKVIAW
                     RRDIHQNPELGNREVRTAALVADHLRGLGFDEVRTGIAHTGVVGILRGGL"
BASE COUNT          648 a         1842 c         1872 g          754 t
ORIGIN      
        1 gcgatcgtgg tcggcgatcc ggccgagcac gccgatgcct tcgccgccag ccgcgcggcg
       61 atcaccaagt caggcaccag caccttgcag ctcgcgctcg ccggcgtgcc gatggtggtg
      121 tgctaccggg tcaacccgct gaccgcgttc ctcgcccgcc gcctgatcac ggtcgccaac
      181 gtcgccctgg tcaacctgct ggccgatgag ccggtcgtgc ccgagctgct gcaggagcgc
      241 tgcacgccgc aaaacatcgc cgacgcgctc gatgcgctga tcgcggacga ggcggcacgc
      301 agcgcccagc gcgcggcgct ggcgaaggtc gccgccggcc tcggcaccgc cgccggcgcg
      361 cccagcgagc tcgccgcgcg gatcgtgctc gacgcgatcg aggcgggcgc cggcgcggcc
      421 cggcgcaagg ccggcgcgtg agcgcggtcg agactcaggt gacgcgcggc gcggtcacct
      481 tccgcgccga gggcatctcg aaggtctacc gtaccggcgc ggtcgcggtg cacgcgctgc
      541 gcggcgtcga catcgcgttc tatgccggcg agttggcggt cctgctcggc ccctcgggca
      601 gcggcaagtc gaccttcctc aacatcctgg gtggcctcga ccggccgacc gaaggccgcg
      661 tgttcttcga cgaacacgag ctgaccgccc tcggcgatgc ggccctgacc gagttccggc
      721 gcaaccatgt cggcttcgtg ttccagttct acaacctgat cccgagcctg accgcgcgcg
      781 agaacgtggc cctggtcacc gagatcgcgc gccgcccgat caggcccgag gacgcgctcg
      841 ccatggtcgg gcttggcgcc cgcctcgacc acttcccggc cgagctgtcg ggcggcgagc
      901 agcagcgggt cgcgatcgcg cgcgccatcg ccaagcagcc cgaggtgctg ttgtgcgacg
      961 agccgaccgg cgcgctcgac agcaacaccg gagtggtcgt gctcgaggcg atcgaccggg
     1021 tcaaccgcga gctcggcacg accaccgcgc tgatcaccca caacgccgcc atcgccggca
     1081 tggccgaccg cgtgatcacc ttcgccgacg gccgcatcgc cggcgtgcgc gacaacgcac
     1141 cgcgcctcgc gcccgctcag atcagctggt agggtgcggc cccggatgac ctctctcgac
     1201 cgcaagctgg tgcgcgacct gtggcacatc cgcggccagg cgcttgcgat cgcgctgatg
     1261 atggcggcgg gcgtggcgat gtcggtgatg tcgctcggca tgctgcgctc gctcgaggaa
     1321 acgctcggcg cctattacga gcgctaccgc ttcgccgacg tgttcgcgca ggtcaagcgt
     1381 gctccggact ggctggcgac ccgggttgcc gccatccccg gggtgggcgt ggtcgagacc
     1441 cggatcgtcg ccgacgtgac cttgaacgtg cccgggctgc tcgagccggc gaccggacgc
     1501 ctgatctcgc tgcccgagcg gggcgcgccg cacatcgacg acatcgccat ccgccagggc
     1561 cgccggctcg cccccggccg cgccagcgag gtggtagcga gcgaggcgtt cgccgaggcg
     1621 aaccggctgg tgcccggcga tacgttcagc gcggtgatca atggcgtcga gcgccggctc
     1681 accatcgtcg gcatcgcgct gtcgccggaa tatgtctact cgatcgggcc cggcgcgctg
     1741 gtcccggacg accgccgctt cggcgtgctg tggatgggca gccgcacgct ggccgcggcg
     1801 ttcgacatgg acggcgcgtt caacgacctc gcgctggcgc tgctgccggg cgcctcgcag
     1861 gacgaggtgg tcgcgcgcct cgaccagctg ctcgaccctt acggcggcac cggcgcctac
     1921 gcgcgcgagc accagcagtc gaactggttc ctgtccaacg agttgaagga gctgaccacg
     1981 ctcgccacca tcgcgccgcc gatcttcctc ggcgtcgccg cgttcctgct caacgtcgtc
     2041 gcctcgcgcc tggtcgagac ccagcgcgag caggtcgggc tcttgaaggc gttcggctac
     2101 ggcaacctcg cggtcgcctg gcattacgcc aagctggtcg gcgtgatcgt gatcgtcggc
     2161 gccgcgctcg gcgtcggcgc cggcatctgg ctcggccgcg ggctggccga gctgtacacc
     2221 cagttcttcc gcttcccgtt ccttcactac cagctcggcg gccaggcgct ggcggtgccg
     2281 gtgctggcca gcatcgccgc cgccgcgctc ggcgccttcg ccgcgctcag gcgcgtgttc
     2341 aggctcgcgc ccgcggtcgc catgcagccg ccggcgccga tgcgctacgg ccgcaccggc
     2401 gccttcgggc tccggctcgt gaacgcccta tcgccgccgt cgcggatgat cgcccgccac
     2461 atcgtccgcc gtccgctgcg ctcggcgctg accgtggtca gcctgtcgag cgcggtggcg
     2521 gtgctgatcg ccgcgatgtt catgctcgat gcgatcgatt acatgctcga ggtccatttc
     2581 caccagagcc agcgccagga cctgaccgtg agcttcaccg agcccgagcc cggccgcgtg
     2641 gtccatgacg cggcgcgcct gccgggcgtg ctgcgcgccg agccgttccg cgcggtgccg
     2701 gtgcggctgc gcttcgggca tctgagcgag cgcacctcta tccagggcgt cgatccggcg
     2761 gcgaccctgg gccgccagct cgaccacgcg ctcgcgccgc tgtcggcgcc gcccgcgggg
     2821 ctgatgctgt cgagctggct ggcgggcgag ctcggcgtcg gcctcggcga caggatcacc
     2881 gtcgaggtga tggaagggcg ccgcccggtg cgcgcgctgc cggtgaccgc gctggtcgag
     2941 gagctgatcg gcgcccgcgc ctacatgcgc atggacgcgc tcaaccggct gatgggcgag
     3001 gggccttcgg tctccagcgt caatctccag atcgaccccg aacggagcgc ggacctctac
     3061 cgccggctca aggagactcc cgccgtcgcc gacaccgtga tcctgaccgc ggcgctgcag
     3121 agcgtgcgcg acacgctcgg cgagacgctg ctcaagatga cctccttcca gatcgccttc
     3181 gccgccatca tcgccttcgg cgtggtctac aacagcgccc gcatctcgtt gtccgagcgc
     3241 gggcgcgagc tcgcctcgct gcgcgtgatc ggattccgcc acggcgaggt atcgttcatc
     3301 ctgctcggcg agctggcggt gctgacgctg ctggcgctgc cgctcggctg cgtgatcggc
     3361 cgcggcctcg cctgggcgtt ggcggtcggc ctgcagagcg acctctaccg cgtgccgctg
     3421 gtggtcgagc gctcgaccta cggcctgtcg gtggcactgg tggtcgcggc ttgggtggcg
     3481 tgcgcgctgg tggttgcatt gcgggttcgc cgcctcgacc tgatcgccgt gctcaagacg
     3541 cgggagtgat cggcgatgtc gggccccgca cggcgcatgc tcgccaccgt cgcggccgcg
     3601 ctggtggtcg ccgcggcgct cgcctgggcg ttctggcccg agcccgtgcc ggtcgacctt
     3661 gcggtcgcgg cgcgcgcgcc gatgcaggtc accgtcgacg aggagggcga gacccgcgtg
     3721 caagacatct acgtggtctc ggtgccgctc accggctggc tcaggcgggt ccaggtcaag
     3781 gccggcgacg cggtcgacgg cggcaagacc gtgatcgcca ctatgcagga gaccgagccg
     3841 agctttctcg atgtgcgctc ccgccgccaa ggcgagtcgc gggtgcgcgc ggcggaagcg
     3901 gcctatgcgc tcgccgaggc cgagcgcaag cgcgccctgg ccgagctcga ctatgcccgc
     3961 tccgagtggc agcgcgcgca gacgctggcc gagcgcggca acatctcgac cgccgcccgc
     4021 gaccgcgccg agctcgcgct gcgcagccag gaggcggcgc tcgccaccgc cgaggccgcg
     4081 gtcaatgtgc gtgcgtccga ggtcgagacc gcgcgcgccg agctgatcgc gccgacggtg
     4141 ggcggcttcg gcgacgagcc ggaaggcgtc gaattgggcg ttgcggtgcg ctcgccggtg
     4201 agcgggcggg tgctgcgtgt gatcaaggag agcgcgggcg tggtccagag cggcatgccc
     4261 ctggtcgagg tcggcgaccc ggcgaacctc gaggtggtgg tcgacctgct gtccgaggac
     4321 gcggtcaagg tgggcgcggg cgatccggtg ctgatcgagc agtggggcgg ccagggcgag
     4381 ctcgccggcc gggtgcgccg ggtcgagccc tacggcttca ccaaggtctc ggcgctcggc
     4441 atcgaggagc agcgggtcaa cgtggtgatc gacttcaccg atgcgcgcga gaaatggcag
     4501 gcgctcggcc acggcttccg ggtcgaggtg cggatcgtca tctggcaggc ggacgacgtg
     4561 ctccaggtgc cgatcggcgc catgttccgc atcggcgacg cgtgggcggt gttcgtcgat
     4621 accggcggcc gcgccgcccg ccgcaccata gaggtcggcc acctgaacga ccgcaccgca
     4681 gaggtgctgt cggggctgga cgagggcgag gccgtcgtcc tccaccccag cgaccgcgtc
     4741 gccgagggcg cgcgcatctt gaagcggctg gtggagtgac cgcgccggtg cccctggtct
     4801 gctatgctgg gcgcggccaa cgagggagag ggcgatgagc ggcttggcgc ggtgggcatg
     4861 ggtgatcggc gtggtcgcgg cagcggcggc gccggcgcgc gcggcggact acgacgcggc
     4921 aatcgcggca acggcggcgg cgatcgaggg caaggtgatc gcctggcggc gcgacatcca
     4981 ccagaacccc gagctcggca accgcgaggt ccgcaccgcc gcgctggtcg cggatcatct
     5041 gcgcggcctc ggcttcgacg aggtgcgcac cggcatcgcc cacaccggcg tggtcggcat
     5101 cctgcgcggc gggctg
//
LOCUS       DASWHU010000309         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_558153, whole genome shotgun sequence.
ACCESSION   DASWHU010000309 DASWHU010000000
VERSION     DASWHU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558153"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGB88_11415"
     CDS             <1..486
                     /locus_tag="VGB88_11415"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX9648100.1"
                     /translation="GHQIVAVGWTPVADAASAALFAGRIEAAGRQMYGETSIPHALRF
                     CMELIESNGIEGQRKAIDLSGDGPTNFGRPPDEMRDRVNAAGIAINGLAILNEFPHLD
                     DYFAAHVVGGPGAFLEVAADYRDFAEAIRRKLIQEIEGVPVGYLVPNSRSPASPNPGT
                     M"
     gene            complement(432..1064)
                     /gene="upp"
                     /locus_tag="VGB88_11420"
     CDS             complement(432..1064)
                     /gene="upp"
                     /locus_tag="VGB88_11420"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9648101.1"
                     /translation="MPENVTVVDHPLVQHKLTLMRRKEASTQEFRQLLREIGMLLAYE
                     VTQDLPVTAERIETPLAVMEAPMLAGKKLCLVSILRAGDGILEGMLDLLPSARVGHIG
                     LYREPASFVAVEYYFKVPEDLAERLVIVVDPMLATGNTAVAAVQRLKDAGGQAIKFVC
                     LVAAPEGIAVFHAAHPDVPVFAAAIDRGLDEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1099..2340)
                     /locus_tag="VGB88_11425"
     CDS             complement(1099..2340)
                     /locus_tag="VGB88_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HEX9648102.1"
                     /translation="MKALHQPAAALAWLRSPAAIRSRCAALLELAERDRLDHFAVDAD
                     RIDAAADYVAEVIAESYPTLEVPLHSRWRQFSAGGIDRWGALARGLANTAPDEVARIR
                     IDLAVTSVLLDAGAGAGWRFREPGSGACLGRSEGLAAASLHLFAAGTLSARPGAPLRA
                     DAAALARIDARSLAAAFQVGDDNPLVGLDGRAAVLRRLGEALAAAPDMFGATAPRIGN
                     LFNYLAGRARDGRVPAPAILDAVLRALGAVWPGRPGVDGVSLGDVWRHPAIRTRDLTD
                     GLVPFHKLAQWLTYSLVEPLAEAGLEVTELDALTGLAEYRNGGLLVDLGVLAPKHDAV
                     LAEVHPVDGEIVVEWRALTVALLDRLADAVRARLDVDAASLPLVKLLEGGTWRAGRKI
                     AASLRADGGPPIRVASDGTVF"
     gene            complement(2348..3580)
                     /locus_tag="VGB88_11430"
     CDS             complement(2348..3580)
                     /locus_tag="VGB88_11430"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX9648103.1"
                     /translation="MDIKKPQQRHIRLASHPGAGAGKAPPIAWGAASAAERGPVIGTL
                     TDLARRNVIGAHAGGYALYRALAIAAQSLDPVHVPDLTDTAPAATIGPFPQWADADRI
                     VSLDPYGHQVAEVFGDRLKDGWNIRPTIAVAKARIRMAELADAVRAGRLKADGELLTE
                     KGDVRVIKAAIEPVWYLPGIAKRCGVDEHALRRALFEHTGGMFPELVTRPDLKVFLPP
                     IGGVTVYLVGEVGALTDPHRPLACRVHDECNGSDVFGSDMCTCRPYLAHGIEVCVATA
                     QAGGAGLIVYNRKEGRALGEVTKFLVYNARKRQDGGDRAEAYFQRTECVAGVQDVRFQ
                     ELMPDVLHWLGITRIDRFVSMSNTKYDAIVRQGIEIAERVPIPPELIPDDAHVEIDAK
                     KAAGYFSADGPPAAPRGR"
     gene            complement(3772..>4057)
                     /locus_tag="VGB88_11435"
     CDS             complement(3772..>4057)
                     /locus_tag="VGB88_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9648104.1"
                     /translation="LWQLEQPAAAVANGVFIGAINRVGEEAPWNIGRFYGSSYVVDPR
                     GKIIAQASESDDELVVADMDLDQIREVRELWQFYRDRRPETYGDLVDLRK"
BASE COUNT          581 a         1486 c         1438 g          552 t
ORIGIN      
        1 ggccatcaga tcgtcgccgt cggctggacg ccggtcgcgg acgcggcgtc ggcggcgctg
       61 ttcgccggcc gcatcgaggc ggcggggcgc cagatgtacg gcgagacctc gatcccgcac
      121 gcgctccgct tctgcatgga gctgatcgag agcaacggca tcgagggcca gcgcaaggcg
      181 atcgacctct cgggcgacgg gccgaccaac ttcggccgcc cgccggacga gatgcgcgac
      241 cgggtgaatg ccgccggcat cgccatcaac gggctcgcca tcctcaacga gttcccgcac
      301 ctcgacgact acttcgccgc ccacgtggtc ggcgggccgg gcgcgttcct cgaggtcgcc
      361 gccgactacc gcgacttcgc cgaggcgatc cggcgcaagc tgatccagga gatcgagggc
      421 gtcccggtcg gctacttggt gccgaacagc cggtcgccgg cgtcgcccaa tccgggcacg
      481 atgtagccgt gctcgtcgag cccgcggtcg atggcggcgg cgaacacggg cacgtcggga
      541 tgggcggcgt gaaacaccgc gatgccctcg ggcgccgcca ccaggcacac gaacttgatc
      601 gcctggccgc ccgcgtcctt gagccgctgg accgcggcga ccgccgtgtt gccggtcgcc
      661 agcatcgggt cgaccacgat caccagccgc tcggccaggt cctcgggcac cttgaagtaa
      721 tactcgacgg cgacgaacga ggccggctcg cggtacagcc cgatatgccc gacccgcgcc
      781 gacggcagca ggtcgagcat gccttcgagg atgccgtcgc cggcgcgcag gatcgacacc
      841 aggcacagct tcttgccggc cagcatcggc gcctccatca ccgcgagcgg cgtctcgatg
      901 cgctccgcgg tcaccggcag gtcctgggtc acctcgtaag cgagcagcat gccgatctca
      961 cgcaggagct ggcggaactc ctgcgtcgac gcctccttgc gccgcatcag ggtgagcttg
     1021 tgctggacca gcgggtgatc gacaacggtc acgttctcgg gcatggcacg gaccttgcgg
     1081 ggcgcgagcg ctaccaaact agaacaccgt gccgtcgctg gcgacgcgga tcggcggccc
     1141 gccgtcggcg cgcaagctgg cggcgatctt gcgccccgcc cgccaggtgc cgccctcaag
     1201 cagcttgacc agcggcaggc tggccgcgtc gacgtcgagc cgcgcgcgca ccgcatccgc
     1261 cagccggtcg agcagcgcca cggtcagcgc ccgccactct accacgatct cgccgtccac
     1321 cggatggacc tcggccagca ccgcgtcgtg cttcggcgcc agcacgccga ggtcgaccag
     1381 caggccgccg ttgcgatact cggcgaggcc ggtgagcgca tcgagctcgg tgacctcgag
     1441 ccctgcctcg gcgagcggct cgaccagcga gtaggtcagc cactgggcga gcttgtggaa
     1501 cggcaccagg ccgtcggtga ggtcgcgcgt gcggatcgcc ggatgccgcc agacatcgcc
     1561 cagcgacacg ccgtcgacgc ccggccgtcc gggccagacc gcgccgagcg cgcgcagcac
     1621 cgcatcgagg atggccggtg ccggcacccg cccgtcccgc gcccgcccgg ccagatagtt
     1681 gaacaggttg ccgatcctgg gcgcggtggc gccgaacatg tcgggcgccg cggcaagcgc
     1741 ttcgcccagc cggcgcagta ccgccgcccg accgtctagc ccgaccagcg ggttgtcgtc
     1801 gccgacctgg aacgccgccg ccaggctgcg cgcatcgatt ctcgccagcg ccgcggcgtc
     1861 ggccctgagc ggcgcgcccg ggcgcgccga cagggtgccc gcggcgaaca aatgcaagct
     1921 tgccgccgcg aggccttccg agcgcccgag gcaggcgccc gacccgggct cgcggaagcg
     1981 ccagcccgcg cccgcgccgg cgtcgagcag gacgctggtg accgcgaggt cgatgcggat
     2041 gcgcgcgacc tcgtcgggcg cggtgttggc gagaccgcgg gcgagcgccc cccagcggtc
     2101 gatgccgccg gccgagaact gccgccagcg gctgtggagc ggcacctcga gcgtcgggta
     2161 cgactccgcg atcacctcgg cgacgtaatc cgcggcggcg tcgatccggt cggcgtcgac
     2221 ggcgaagtgg tcgagccggt cgcgttcggc gagctcgagc agcgccgcgc agcggctgcg
     2281 gatcgccgcc ggcgagcgca gccaggcgag cgccgccgcc ggctggtgca gcgctttcaa
     2341 ccggccccta ccgtccgcgc ggcgcggccg gcgggccgtc ggcggagaag tagccggcgg
     2401 ccttcttggc gtcgatctcg acatgggcgt cgtcggggat cagctcgggc gggatcggca
     2461 cccgctcggc gatctcgatg ccctggcgga cgatggcgtc gtacttggtg ttgctcatcg
     2521 acacgaagcg gtcgatgcgc gtaatcccga gccagtgcag cacgtcgggc atgagctcct
     2581 ggaagcgcac gtcctggacg ccggcaacgc attcggtgcg ctggaagtag gcctcggcgc
     2641 ggtcgccgcc gtcctggcgc ttgcgcgcgt tgtagaccag gaacttggtc acctcgccga
     2701 gcgcgcggcc ctcctttcgg ttgtagacga tcagcccggc gccgccggcc tgggcggtgg
     2761 cgacgcacac ctcgatgccg tgggcgaggt agggccggca ggtacacatg tccgagccga
     2821 acacgtccga gccgttgcac tcgtcgtgca cccggcaggc gagcgggcgg tggggatcgg
     2881 tgagcgcgcc gacctcgccg acgaggtaga ccgtgacgcc gccgatcggg ggcaggaaca
     2941 ccttgaggtc cggccgggtg accagctcgg ggaacatgcc gccggtgtgc tcgaacaggg
     3001 cgcggcggag cgcatgctcg tcgaccccgc agcgcttggc gatgcccggc aggtaccaga
     3061 ccggctcgat cgcggccttg atcacccgca cgtcgccctt ctcggtcaag agctcgccgt
     3121 cggccttcag gcggccggcg cgcaccgcgt cggcaagctc ggccatcctg atccgcgcct
     3181 tggcgaccgc gatcgtcggg cggatgttcc agccgtcctt gagccggtcg ccgaacacct
     3241 cggcgacctg gtggccgtag gggtcgagcg agacgatccg gtccgcatcg gcccattgcg
     3301 ggaacggccc gatcgtcgcc gccggcgcgg tgtcggtgag gtccggcacg tgcaccgggt
     3361 cgaggctctg cgcggcgatc gcaagcgcgc gatagagcgc gtagccgccg gcatgggcgc
     3421 cgatcacgtt gcgccgggcg agatccgtga gcgtgccgat caccggcccg cgctcggccg
     3481 cgctggccgc gccccaggcg atcggcggcg ccttgccggc gcccgcgccc ggatgcgagg
     3541 cgagcctgat atgcctctgc tgcggcttct tgatgtccat cggacgatgc ctccgtctcg
     3601 gtggcgtccc gcggccggtg cccttacccg gccccagaac gcaaaacagc cagcagtttt
     3661 tcccgctggc tgcgcgcgga cggcccaaag ccgccgtcga ggcgcgattt gccggaagcg
     3721 aatgaatcta caccgaaccc gccccgcgca caatcgccgc ccaaagaccg gctacttgcg
     3781 caggtcgacc aggtcgccgt aggtctccgg ccgccggtcg cggtagaact gccacagctc
     3841 gcgtacctct cttatctggt cgaggtccat gtccgcgacc accagctcgt cgtcggactc
     3901 gctcgcctgg gcaataatct tgccgcgcgg gtcgaccacg taggacgagc cgtagaagcg
     3961 gccgatgttc cacggcgcct cctcgccgac ccggttgatc gcgccgatga acacgccgtt
     4021 ggcgaccgcc gccgccggct gctcgagctg ccacaaa
//
LOCUS       DASWHU010000310        12209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1281396, whole genome shotgun sequence.
ACCESSION   DASWHU010000310 DASWHU010000000
VERSION     DASWHU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281396"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..403
                     /gene="arsC"
                     /locus_tag="VGB88_11440"
     CDS             56..403
                     /gene="arsC"
                     /locus_tag="VGB88_11440"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006000.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX9648105.1"
                     /translation="MAVTIYHNPRCSTSRRTLELLRARGIEPRVVEYLKDPPDAGTLK
                     RLLRMLGLKPRELMRRKESAYTDNRLDDPGLGEEALVRAMVAHPILIERPIVVSGAKA
                     ALGRPPESVLDIL"
     gene            408..1118
                     /locus_tag="VGB88_11445"
     CDS             408..1118
                     /locus_tag="VGB88_11445"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX9648106.1"
                     /translation="MSDYEHGGGGWPMWPLIVAALVLLSVGVVALDRLAPGVLGSEAG
                     AMRFVYLILLLLILGPALFAGPLARALRHLVVWVAAGAVLVLGYTLWQRGQLTGETVG
                     GELLPAQDSAAHENQARFVANSDGDFLITARVDHSNVRFRVDTGASDVMLTRADAIRI
                     GFNPDTLDYTRAYMTANGKVFAAPVRIGRIVVGDVTIRDVVASVDNGKLDVSLLGMSF
                     LSRLSGYEVRDGVLILHQ"
     gene            complement(1123..2754)
                     /locus_tag="VGB88_11450"
     CDS             complement(1123..2754)
                     /locus_tag="VGB88_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9648107.1"
                     /translation="MGANPYQVGLDKTPANHRPLTPLSFLERACTVYPERTAWVHGGK
                     SVDYRTFDARCRRLAGALSRRGIGAGDTVAVMAPNVPAILEAHYGVPLAGAVLNALNV
                     RLDAGAIRFILEHGEAKLVISDREFSPVIKDALAGMARPPVVIDYDDPSYRGAGERLG
                     EVEYESFLAEGAADFEGVPVEDEWAAISLNYTSGTTGNPKGVVYHHRGAFLNAIGNLM
                     VWAMPPHPVYLWTLPMFHCNGWCFPWTVTALAGTHVCLRRVEAGQILGAIAEHGVSHF
                     CGAPIVLNMVANADAAALARINRRIEVMTAAAPPPPPVIEAMERNGFHVTHVYGLTEC
                     YGPAVVCAWHEEWDALAIEQQARMKARQGVRYPVLEGLAVMAPETMAPVAADGATMGE
                     VMMRGNVVMKGYLKNPKATEEAFAGGWFHTGDLGVIHADGYIELKDRSKDIIISGGEN
                     ISSIEVEGTLYRHPAVMEAAVVARPDDTWGETPCAFVELKPGAAPVSEADLIAFCRAR
                     MARFKAPKTVVFGPLPKTSTGKIQKFVLREKARNL"
     gene            complement(2759..3625)
                     /locus_tag="VGB88_11455"
     CDS             complement(2759..3625)
                     /locus_tag="VGB88_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9648108.1"
                     /translation="MTAPCQGPDPHTRQPRLEVPAGACDCHAHVFAPLSEAAMVAARA
                     YTPPPAPLEAYRHMLSILGIQRAVIVQPSVYGADNRVAMAAVRAYGRDCRAVVVVDPG
                     IVEAELKAMDAAGARGVRLNLQYAGGVGLDAAAALARRIAPLGWHLQLLLDARDLPDL
                     LPAIRALPVEVVVDHMGHMNAAHGIGHPGFRALLEALAGGRCWVKLSGNYRITAAGAP
                     YDDAIPFARALVAANPERCVWGTDWPHPALEAPMPNDADLLDALAAQVPDEAARARIL
                     VDNPARLYRFDG"
     gene            complement(3635..4147)
                     /locus_tag="VGB88_11460"
     CDS             complement(3635..4147)
                     /locus_tag="VGB88_11460"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9648109.1"
                     /translation="MSCEAPATGHSRPGHRAAAALALAAMLSAPPAGAAPACESAGPA
                     EAQALAERAAALLEQAGPKRAFTTFMIADGGFIDRDLYVFVIDFDGTLWANGAFPNSV
                     GSAAVDAHTADGHYYIREMISVAAERGTGWVEYEWINPCTGEFEPKVSYVIRVGRFII
                     GVGAYGTVGT"
     gene            4420..7083
                     /locus_tag="VGB88_11465"
     CDS             4420..7083
                     /locus_tag="VGB88_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9648110.1"
                     /translation="MADDVLAAVLDTVAAWIVRCDSRGRLIDVNQACLAATGYDRSDI
                     VGRPVWDTVIPPDQADEVKRVLADLVTGKQPSRFINDWVAKDGSRRTVAWSNCVTTNK
                     RGAVDAIIGTGIDITDSVKVERHLGDNERFVQALVDNALDLITVIDADGTIRFSSPSI
                     ERVLGYKLEERVGRNAFEFVHPDDAERVRQAIARGLKPGREIETAEYRYRAADGTWHW
                     LESVGRNLIADPAVGGIVINSRDVTIRRELEQRYQAIVEQSPDAIMVIDPETTLMLAF
                     NRAACEVHGYSAEQMRALTIADYDFGETPDEIRRRIERVLAAGTDDFETRMRRGDGEI
                     RDVQVRLSLIEISGRPVLNAIVRDITLHKRAQEALAKSELRFRDLVETTPDWVWQADE
                     NGVFTYASPQVKQLLGHEPEAVLGHTPYDFMPPEEAERVRAEVTGLTRGQGPFEHLQN
                     VMRHKDGRLVVLETSGVPVLAPDGAFRGHRGIARDVTQSRAAMRELQRTNRLLVLIRR
                     CNQELVRARDETALLASICRLTVEEGGYRFAYVGFVDGDRKETLRLLASYGEGGGFLD
                     ALRLPDGEDRIADDVPRRAAADSRGVPAVVRDIAANGKATPWRDAALAAGFASRASFA
                     LKVGDRVIGVLNAFSAAADVFDDDEIRLLRELADDLAFGIVSLRETGRRLQAEQRQLE
                     LERQREQALIQTIDAIALAAEARDPYTSGHEKRVARLAKAIAVEMGLPADQCEGIGLA
                     GAIHDIGKLHVPMEILARPARLNAAEFELVKAHAPIGYNILKSIQFSWPVAEAVYQHH
                     ERWDGSGYPNGLKGEAIMLEARILGVADVIEAMASHRPYRPALGIDAALLHVLQNRGV
                     LYDPRVVDACLVVCKESDPFFDDAAPSPMSN"
     gene            7095..7427
                     /locus_tag="VGB88_11470"
     CDS             7095..7427
                     /locus_tag="VGB88_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648111.1"
                     /translation="MKASQRCDHCQFWVSLWSLKRKAAEPGQDGAERRSKRGQCHRRA
                     PQSSALTVFWPWTLAEDRCGEFEPWPDERRKPAHGGKVMAPGPAGAEHRQVPPHGARH
                     EHLGTGVR"
     gene            7833..10106
                     /locus_tag="VGB88_11475"
     CDS             7833..10106
                     /locus_tag="VGB88_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648112.1"
                     /translation="MTASQAGVPARRSPRAHAAITYAVLFVIALAPIVALGVYAHRAA
                     STAIDRLVRSNNLAAATLTRGLVEQEFTQWVGNLVGFARQPGLIDSVRRHDAPAVASR
                     LEPLVESQPHIVRAFVTDPEGLLWSDYPPAPESLGQTFAHRDWYRGLSASWQPYVSAV
                     YRRHAAPRLLLVAIAAPIRDPDSDRVIGAVVYQVTPEALTRTLRQLVVGHDGFVYLID
                     HAGALVAHPGIDLQAREYNDYADSRPVTEARAAGVTTVEYADPLSRADMLATAVTATV
                     AGHQWVVVAQQPLAAAFRATRLLAAQIGGAGLQVFAALSALFFVLGGYHVRMRRLNLR
                     LGHLNRALSRENQDRRLAEQALSQANDQLERRVEDRTRELRETEQQLVQTQKMEAIGR
                     LAGGIAHDFNNMLTVIIGYADLILHSPPKDESLRAKVVQIHDAGVRASTLTKQLLAFS
                     RKQVLKPEIIDLNDVLRRMDQMMRRLIGEDIDLLTTFDPDLDPVEADPGQIEQIIMNL
                     AVNARDAMPGGGKLTIGTANVDLDPDYARTHPGAQPGWHVMIAVADTGCGFDAATRAR
                     IFEPFFSTKKDSGGTGLGLSTVYGIVKQSGGNIWVYSEPGKGTTFKVYLPRAEQAGAE
                     AKARIEAQAPSGGTETILVVEDEDGVRSLVCDVLTSAAYTVIDTGEVERAIEICRRYD
                     GPIDLLLTDVVMPGMNGRELATAALALRPDLRVVYMSGYTDDAIVHHGVLDPGTAFIE
                     KPVTPRALLEKIRDALH"
     gene            10263..10475
                     /locus_tag="VGB88_11480"
     CDS             10263..10475
                     /locus_tag="VGB88_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648113.1"
                     /translation="MHITRHDHDHAQALAERGRRGEIYLTPSDLKALDTLVAAWIGQI
                     PGYPPTISENSYRRARQLIEQYEESR"
     gene            complement(10693..11073)
                     /locus_tag="VGB88_11485"
     CDS             complement(10693..11073)
                     /locus_tag="VGB88_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648114.1"
                     /translation="MSEPRPAIEHRDSLPRYGPPGHAGTVNVRLVDAGFGGGFELVHG
                     TVAPGGRAERHNHGREYQVIYVVEGTGRVALGDQPARTCGPGTVVRIPPGLDHEVVND
                     GTVPLKVLIVYSPPLARRADTALD"
     gene            complement(11070..>12209)
                     /locus_tag="VGB88_11490"
     CDS             complement(11070..>12209)
                     /locus_tag="VGB88_11490"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9648115.1"
                     /translation="DVIKVESPEGDTARTSGPEPTRGRGTLFIGSNRNKRSLVLDLKQ
                     ADGRDALLALAARADVFLHNMRPQAIARLGLAYAEVSSASPAIVYCAAYGFRAGGPYS
                     HKPAFDDMIQAASGFAALQGRNLGRPVYATSIIADKICAMAAAQAIVMALFSRARSGR
                     GQAIEMPMFETLVAFNMVEHLYGRTFEPARGPAGYPRALSPDRRPYRTADGYIGVLPY
                     TERQWQAFLGVAGRPELIDDARFASYGARLANVDALYAEIAACLAGRTSAEWLAALDE
                     VNVPCTVVNAPNDLPDDPHLAAVGFWEMREDPELGTLRLPGIATRFSDTPGAIRRLPP
                     RLGEHSLEVLREIGLSEQRIRHMLDRGVTVQAPPLAAPAEPEDVP"
BASE COUNT         1838 a         4292 c         4326 g         1753 t
ORIGIN      
        1 gggtcaccgt cgccgcgacc aagtggccgg cgcgcacgac acgagaggac gagccatggc
       61 cgtgaccatc taccacaacc cgcgctgcag cacctcgcgc cggaccctgg agctgctgcg
      121 cgcgcgcggc atcgagccca gggtggtcga gtatctcaag gacccgcccg acgcgggcac
      181 gctcaagcgg ctgttgagga tgctcggcct taagccgcgc gagctgatgc ggcggaagga
      241 gtccgcctac acggacaacc gcctcgacga cccgggcctc ggcgaggagg cgctggtccg
      301 ggcgatggtc gctcacccga tcctgatcga gcgcccgatc gtggtcagcg gcgccaaggc
      361 ggcgctgggt cggccgcccg agtcggtgct cgacatcctg tgaggcgatg agcgactacg
      421 aacacggcgg cgggggctgg ccgatgtggc cgctgatcgt ggctgcgctg gtgctgctgt
      481 cggtgggcgt ggtggcgctc gaccgcctgg cgcccggggt gctcggcagc gaagcgggcg
      541 cgatgcgctt cgtctatctg atcctgttgc tgctgatcct cggaccggcg ctgttcgccg
      601 ggcccttggc gcgcgcactc cgccacctgg tggtgtgggt ggcggcgggc gcggtgctgg
      661 tgctcggcta cacgctgtgg cagcgcggcc agctcaccgg cgagacggtc ggcggcgagc
      721 tgttgccggc gcaggattcg gcggcgcacg agaaccaggc ccgcttcgtc gccaacagcg
      781 acggcgactt cctgatcact gcgcgggtcg accacagcaa cgtccgcttc cgggtcgaca
      841 ccggcgccag cgacgtgatg ctgacccgcg ccgacgccat ccgcatcggc ttcaaccccg
      901 acaccctcga ctacacccgt gcctacatga ccgccaacgg caaggtgttt gcggccccgg
      961 tgcggatcgg ccgcatcgtg gtcggcgacg tcaccatccg cgacgtcgtg gcctcggtcg
     1021 acaacggcaa gctcgacgtc tcgctgctag gcatgagctt cctgtcgcgc ctgtccggct
     1081 acgaggtccg cgacggcgtc ttgatcctcc atcagtagcg cgctaaaggt tgcgcgcctt
     1141 ctcgcgcagc acgaacttct ggatcttgcc ggtggaggtc ttgggcagcg gcccgaacac
     1201 caccgtcttg ggcgccttga agcgcgccat gcgcgcgcgg cagaaagcga tcaggtccgc
     1261 ctcgctgacc ggcgcggcgc cgggcttgag ctcgacgaag gcgcacggcg tctcgcccca
     1321 ggtgtcgtcg ggacgcgcca ccaccgccgc ctccatcacc gcgggatggc ggtagagcgt
     1381 gccctcgacc tcgatcgagg agatgttctc gccgcccgag atgatgatgt ccttggagcg
     1441 gtccttgagc tcgatatagc cgtcggcgtg gatgacgccg aggtcgccgg tgtggaacca
     1501 gccgccggcg aacgcttcct cggtcgcctt cgggttcttg aggtagccct tcatgaccac
     1561 gttgccgcgc atcatcacct cgcccatggt ggcgccgtcg gcggcgaccg gggccatggt
     1621 ctcgggcgcc atcacggcga gaccctcgag caccggatag cgcaccccct ggcgcgcctt
     1681 catgcgcgcc tgctgctcga tcgcgagggc gtcccattcc tcgtgccagg cgcacaccac
     1741 cgccgggccg tagcactcgg tcaggccgta gacatgggtg acgtggaagc cgttccgctc
     1801 catcgcctcg atcaccggcg gcggcggcgg cgcggcggcg gtcatcacct cgatgcgccg
     1861 gttgatgcgc gccagcgcgg ccgcatccgc attggcgacc atgttgagca cgatcggcgc
     1921 gccgcagaag tggctcaccc cgtgctcggc gatggcgccc aggatctggc cggcctcgac
     1981 ccggcgcagg cacacatggg tgccggcgag cgcggtcacc gtccacggga agcaccagcc
     2041 gttgcagtgg aacatcggca gcgtccacag atagaccggg tgcggcggca tcgcccagac
     2101 catcaggttg ccgatcgcgt tcaggaacgc gccgcgatgg tggtagacca cgcccttggg
     2161 gttgccggtg gtgcccgagg tgtagttgag cgagatcgcc gcccactcgt cctcgaccgg
     2221 aacgccctcg aagtcggccg cgccctcggc gaggaaggac tcgtactcga cctcgcccag
     2281 ccgctcgccc gcgccccggt aggacgggtc gtcatagtcg atcaccaccg gcgggcgcgc
     2341 catgccggcg agcgcgtcct tgatgaccgg gctgaactcg cggtccgaga tcacgagctt
     2401 ggcctcgccg tgctcgagga tgaagcggat cgcgccggca tcgaggcgca cgttgagcgc
     2461 gttcagcacc gcccccgcca gcggcacgcc gtaatgcgcc tcgaggatcg ccggcacgtt
     2521 gggcgccatc accgccaccg tgtcgccggc gccgatgccg cgccggctga gcgcgccggc
     2581 gaggcggcgg cagcgggcgt cgaacgtgcg gtagtcgacg ctctttccgc catgcaccca
     2641 ggcggtacgc tcggggtaga cggtgcaggc gcgctcgagg aagctcagcg gcgtcagcgg
     2701 ccggtggttg gccggggtct tgtcgagccc gacctggtac gggttggcgc ccatccgctc
     2761 agccgtcgaa gcggtacagc cgggccgggt tgtcgaccag gatgcgcgcg cgcgccgcct
     2821 cgtccggcac ctgtgcggcg agcgcgtcga ggaggtcggc gtcgttgggc atcggcgcct
     2881 cgagcgccgg gtgcggccaa tcggtgcccc acacgcagcg ctcgggattc gccgccacca
     2941 gcgcgcgcgc gaacggaatc gcgtcgtcat agggcgcgcc cgcggcggtg atgcggtagt
     3001 tgcccgacag cttgacccag cagcgcccgc ccgcgagcgc ctcgagcagg gcgcggaagc
     3061 ccggatggcc gatgccgtgg gcggcgttca tgtggcccat atggtcgacc accacctcga
     3121 ccggcagcgc gcggatggcg ggcaagaggt cgggcaggtc gcgcgcgtcg agcaacagct
     3181 ggaggtgcca gccgagcggc gcgatccggc gcgccagcgc ggccgcggcg tcgagcccga
     3241 ccccgccggc atattggaga ttgagccgca ccccgcgcgc acccgccgcg tccatcgcct
     3301 tgagctcggc ctcgacgatg ccggggtcga ccaccaccac cgcgcggcag tcgcgcccgt
     3361 aggccctgac cgcggccatg gcgacccggt tgtcggcgcc gtagacgctc ggctggacga
     3421 tcaccgcgcg ctggatgccg aggatcgaca gcatgtgccg atacgcctcg agcggcgccg
     3481 gcggcggcgt gtaggcgcgc gccgcgacca tcgctgcctc ggacaacggc gcgaacacat
     3541 gcgcgtggca gtcgcaggcg ccggccggga cctcgagccg gggctggcgc gtgtgcgggt
     3601 cgggaccctg gcagggagcg gtcatggccg gaggtcaggt gccgacggtg ccgtaggcgc
     3661 cgacgccgat gatgaagcgc ccgacccgga tcacgtagct caccttcggc tcgaactcgc
     3721 ccgtgcacgg gttgatccac tcatactcga cccagccggt gccgcgctcc gccgccaccg
     3781 agatcatttc gcgaatgtaa tagtggccgt cggcggtgtg cgcatcgacc gccgcgctgc
     3841 cgaccgagtt cggaaacgcg ccgttggccc acaatgtgcc gtcgaagtcg atcacgaaca
     3901 catacaggtc gcggtcgatg aagccgccgt cggcgatcat gaaggtggtg aacgcccgct
     3961 tgggacccgc ctgctcgagc agcgccgccg cgcgctcggc gagcgcctgc gcctcggccg
     4021 ggcccgccga ctcgcaggcc ggggcggcgc cggccggcgg cgccgacagc atggcggcga
     4081 gggcgagcgc cgccgcagcc ctgtgtcccg gccgggaatg gcccgtcgct ggcgcttcgc
     4141 acgacatctc acccgcctct gctcccgacc gcgtcgagcg cgcatcatgg cgcagcccgg
     4201 gccgctccga caaggccccg gtacatccgg acgcactgcc ggcggcgcgg ttacggccgg
     4261 cacctcgggg cgcgctccgg cgcaagcgga agtgcgcaac ggatcgcccc tactcgcttg
     4321 ccgagcattg caagtgccct aatttcctag gttgttatgg aacgcgaagg tctcgtgtta
     4381 cacgcagggg ttgtacacgg gtcgaagcgg aggagcggga tggcggacga cgtgctcgcc
     4441 gccgtcctcg atacggtagc tgcctggatc gtccgctgcg acagccgcgg ccggctgatc
     4501 gacgtcaacc aggcgtgcct tgccgccacc ggctacgacc gttcggacat cgtcgggcgc
     4561 ccggtgtggg atacggtcat cccgccggat caggccgacg aggtgaagcg cgtgctcgcc
     4621 gatctggtca cgggcaagca acccagccgg ttcatcaacg attgggtggc caaggacggc
     4681 tcgcgccgca ccgtcgcgtg gtccaattgc gtcaccacca ataaacgcgg cgcggtcgat
     4741 gcgatcatcg gcacgggcat cgacatcacc gactcggtga aggtcgaacg acacctcggc
     4801 gacaacgagc gcttcgtcca ggcgctggtc gacaacgcgc tcgacctgat caccgtgatc
     4861 gacgccgacg gcaccatccg cttctcgagc ccctcgatcg agcgcgtgct cggctacaag
     4921 ctggaagagc gggtcggcag gaacgcgttc gagttcgtgc atcccgacga cgcggagcgc
     4981 gtgcgccagg cgatcgcccg cggcctcaag cccggacgcg agatcgagac cgccgagtac
     5041 cgctatcgcg ccgccgacgg gacctggcac tggctcgagt cggtcgggcg caacctgatc
     5101 gcagaccccg ccgtcggcgg catcgtcatc aactcgcgcg acgtgacgat ccggcgcgag
     5161 ctcgagcagc gctaccaggc catcgtcgag cagtcgcccg acgcgatcat ggtcatcgac
     5221 ccggaaacga cgctgatgct ggcgttcaac cgggccgcct gcgaggtcca cggctacagc
     5281 gccgagcaga tgcgtgcgct gaccatcgcc gactacgact ttggcgagac cccagacgag
     5341 atccgccggc gtatcgagcg cgtcttggcc gccggcacgg acgacttcga aacccggatg
     5401 cgccgcggcg acggcgagat ccgcgacgtc caggtacgcc tctccctgat cgagatttcg
     5461 ggccgcccgg tgctcaacgc catcgttcgc gacatcaccc tgcacaagcg ggcgcaagag
     5521 gcgctggcca agagcgagct caggttccgc gacctggtcg agaccacgcc cgactgggtg
     5581 tggcaggcgg acgagaacgg cgtcttcacc tatgccagcc cccaggtgaa acagcttctc
     5641 ggccatgagc ccgaggcggt gctggggcac acgccgtacg atttcatgcc gcccgaggag
     5701 gccgagcgcg tgcgcgccga ggtcaccggg ctgacccgcg ggcaggggcc gttcgagcat
     5761 ctccagaacg tcatgcgcca caaggacggc cgcctggtcg tgctggagac gagcggcgtg
     5821 ccggtgctcg cccccgacgg ggccttccgc ggccatcgcg gcatcgcccg cgacgtcacc
     5881 cagagccggg cggcgatgcg cgagctgcag cggaccaatc gtctgctcgt gctcatccgc
     5941 cggtgcaacc aggagctcgt cagggcacgg gacgagaccg cacttctggc aagcatctgc
     6001 cgcctgacgg tcgaagaggg cggctaccgg ttcgcctatg tcggattcgt cgacggcgat
     6061 cggaaggaga cgctgcgcct gcttgcctca tatggcgagg gcggaggttt cctcgacgcg
     6121 ttgcgactcc ccgacggcga agatcggatc gccgacgacg tgccgcggcg agccgccgcc
     6181 gatagccggg gcgtgccggc ggtggtccgc gacatcgcgg ccaacggcaa ggccacgccc
     6241 tggcgcgacg ccgcgcttgc cgccggattc gcctcccggg cgagctttgc cttgaaggtc
     6301 ggcgatcggg tcatcggggt gctcaacgcg ttttcggcgg cggccgatgt tttcgacgac
     6361 gacgagatca ggctgttgcg cgagcttgcc gacgacctcg ctttcggcat cgtgtcgttg
     6421 cgcgagaccg gccggcgctt gcaggcggag cagaggcagc tcgagctgga gcgccagcgc
     6481 gagcaagcgc tgatccagac catcgatgcg atcgcgctcg ccgccgaggc gcgcgacccc
     6541 tacaccagcg gccacgagaa gcgggtcgcc cggctggcca aggcgattgc ggtcgagatg
     6601 ggcctgccgg cggaccagtg cgagggcatc gggctcgccg gcgcgatcca cgacatcggc
     6661 aagctccatg tgccgatgga gattctcgcc cgccccgcgc gcctgaacgc ggccgagttc
     6721 gagctggtca aggcacatgc gccgatcggc tacaacattc tcaagtcgat ccagttctcg
     6781 tggccggtcg ccgaggcggt ctaccagcac cacgagcgct gggacggctc gggatacccg
     6841 aatgggctga agggcgaggc gatcatgctc gaggcccgca tcctcggcgt cgccgacgtg
     6901 atcgaggcga tggcgagcca ccgcccctac cggccggcgc tcggcatcga cgccgcgttg
     6961 ctccacgtcc tccagaacag gggcgttctg tacgatcccc gcgtcgtcga cgcctgcctt
     7021 gtcgtctgca aggagtccga cccgtttttc gacgatgccg cgccgtcgcc catgtccaac
     7081 tgagggatgc gccgatgaaa gccagccaac gatgtgacca ttgccaattc tgggtctcgc
     7141 tgtggtcgct caagcgcaag gccgcggaac ctggccagga cggcgccgaa cgtcgctcga
     7201 agcgcgggca gtgccaccgc cgcgcgccgc agtcgagcgc gctgacggtg ttctggccgt
     7261 ggaccctggc cgaggaccgc tgcggcgagt tcgagccgtg gcccgacgag cggcgcaagc
     7321 cggctcatgg cggcaaggtg atggcgccgg gtccagctgg tgcggagcat cggcaggtgc
     7381 cgcctcacgg cgcccggcat gaacatctcg ggacgggggt tcggtgatga acaggtacca
     7441 gctttgcagc cgttgccggt tctgggtatc gccgcgcggg ccgggccggt tctggtccgg
     7501 cctcggcggc ggcagccggc ggtcgaagcg cggccagtgc cgccggcacg cgccgcgggc
     7561 gagctcgctg acgccgcact ggccgtggac gagggccgac gacgtgtgcg gcgagttcga
     7621 gctgcggccg gacgagcatc gcgatcttga gccgtcgccc gcaccggcga cgggcggcct
     7681 cgagacgatt tcgtgagtga cgcgccggcg cggctgaaac gggcgtcatc gcggccgcgc
     7741 accgtggcgg ccacgactcg aaggcggatg gcgaaggccg gccggatggg cgcatcgcgg
     7801 caccggcagg cgggtgttga gcggatgcgg tgatgaccgc ttcacaggcc ggtgtccccg
     7861 cgcggcggtc gccgcgcgcc cacgccgcga tcacctatgc ggtcctgttc gtgatcgcac
     7921 tcgcgccgat cgtcgcgctc ggcgtctacg cgcaccgggc cgcaagcacg gcgatcgacc
     7981 gcctggtgcg cagcaacaac ctggccgccg cgacccttac ccgcgggctg gtcgagcagg
     8041 agttcacgca gtgggtcggc aacctggtcg ggttcgcgcg ccagcccggg ctgatcgact
     8101 cggtgcgccg ccacgatgcg cctgcggtcg ccagccggct cgagccgctg gtcgagtccc
     8161 agccccacat cgtgcgcgcc ttcgtcaccg atcccgaggg cctgttgtgg agcgactatc
     8221 cgccggcgcc cgagtcgctg ggccagacct tcgcccaccg cgactggtac cggggcctgt
     8281 ccgcctcctg gcagccatac gtctcggcgg tgtatcgccg ccacgccgcg ccgcggctgc
     8341 tcctggtcgc gatcgcggcg ccgattcgag acccggacag cgaccgggtg atcggcgccg
     8401 tggtctatca ggtgacgccg gaagccttga cccggacgct ccgccagctg gtcgtcggcc
     8461 acgacggttt cgtctacctg atcgaccacg ccggcgccct ggtcgcgcat cccggaatcg
     8521 accttcaagc gcgggaatac aacgactatg cggactcgag gccggtcacc gaggcgcgcg
     8581 cggccggcgt gacgacggtc gaatacgcgg acccgctgag ccgcgccgac atgcttgcga
     8641 ccgcggtcac cgcgaccgtc gccggccacc aatgggtggt ggtcgcccag cagcccctcg
     8701 ctgccgcctt tcgcgcgacc cgcctgctcg cggcgcagat cggcggcgcc gggctgcaag
     8761 tgttcgcggc gctgagcgcg ctgttcttcg tgctcggcgg ataccatgtg cggatgcggc
     8821 gcctgaatct gcgtcttggc catctcaacc gggcgctgtc gcgcgagaac caggatcgcc
     8881 gcctggccga gcaggcgctg tcgcaggcca acgaccagct cgagcgacgg gtcgaggacc
     8941 gcacccgcga gctgcgcgag accgagcagc agctggtgca gacccagaag atggaggcga
     9001 tcggccggct cgccggcggc atagcgcacg acttcaacaa catgctgacc gtgatcatcg
     9061 gctacgccga cctgatcctg cacagcccgc ccaaggacga gtcgctgcgg gcgaaggtcg
     9121 tccagatcca tgacgccggc gtccgcgcca gcaccctcac caagcagctt ctggcgttca
     9181 gccgcaagca ggtcctcaag ccggaaatca tcgacctcaa cgacgtcctg agacggatgg
     9241 accagatgat gcgccggctg atcggcgagg acatcgatct tttgaccacg ttcgatcccg
     9301 acctcgaccc ggtcgaagcc gaccccggcc agatcgagca gatcatcatg aacctggcgg
     9361 tcaacgcccg cgacgcgatg cccggcggcg gcaagctgac catcggcacc gccaatgtcg
     9421 acctcgaccc ggactacgcg cgcacgcacc ccggcgcgca gcccggctgg catgtgatga
     9481 tcgcggtcgc cgataccggt tgcggcttcg acgccgcgac ccgcgcgcgc atcttcgagc
     9541 cgttcttctc gaccaagaag gacagcggcg gaaccggcct cgggctgtcg acggtctacg
     9601 gcatcgtcaa gcagagcggt ggcaacatct gggtgtacag cgagcccggc aagggcacca
     9661 cgttcaaggt ctacctgcct cgcgccgagc aagccggcgc cgaggcgaaa gcgcgcatcg
     9721 aggcgcaggc gccttccggg ggcaccgaaa ccatcctcgt ggtcgaggac gaggacggcg
     9781 tccgcagcct ggtgtgcgac gtgctgacct cggccgccta caccgtgatc gacaccggcg
     9841 aggtcgagcg cgcgatcgag atctgccggc gctacgacgg ccccatcgac ctgttgctga
     9901 ccgacgtggt gatgccgggg atgaacggtc gcgagctggc cacggcggcg cttgcgctgc
     9961 gccccgacct gcgggtggtc tacatgtcgg gttacacgga cgatgcgatc gtccaccacg
    10021 gcgttctcga cccgggcacg gcgttcatcg agaagccggt caccccgcga gccctgctcg
    10081 agaagatccg ggacgcgctg cattagatcg gctgtcccgg cgcgcccgcc gcccggtgac
    10141 gctgcgggca ggcggctcga tcccggccgg atcgtactat ttttatccct tgccgatgtg
    10201 gaccaagctt gggcgccgcc gcgagcatgg gtcggcggcc aggcaagccg caagaggtgg
    10261 ctatgcacat cacgcgccac gaccatgacc acgcgcaggc gctggccgag cgtggcaggc
    10321 gcggggaaat ctatctcact ccgagcgacc tcaaggcgct ggatacgctg gtcgccgcgt
    10381 ggatcgggca gattcccgga tatccgccga caatctccga gaacagctac cgccgcgccc
    10441 gccagctgat cgagcaatac gaggagagcc gctagggcga cgcccgccgg cccggccccg
    10501 gcctcctcgc agagtcgccc gaaaagcggg taggctcgca ctcatctact acttttacgt
    10561 gtaaaaccaa cgcttgcgga ccgaccgagg cgtgtcgtcg cgtcgccgtt gcgcggacca
    10621 cgaccggcgg tcgaggcgcg ccgcgcccga gaccgttagg cggctatcgt tcccgtggat
    10681 cgcgggtcac ggtcagtcga gcgcggtgtc ggcgcggcgc gcgagcggcg ggctgtagac
    10741 gatcagcacc ttgagcggca cggtgccgtc gttgaccacc tcgtggtcga ggccgggcgg
    10801 gatgcggacc accgtgcccg ggccgcaagt gcgcgccggc tggtcgccga gcgcgacccg
    10861 gccggtgccc tcgaccacgt agatcacctg gtactcgcgg ccgtggttgt ggcgctcggc
    10921 gcgcccgccc ggggcgaccg tgccgtggac cagctcgaag ccgccgccga agccggcatc
    10981 gaccaggcgc acgttgaccg tgccggcgtg ccccggcggg ccgtagcggg gcaacgaatc
    11041 gcgatgctcg atcgccgggc gcggctcgct catggcacgt cctcgggctc ggccggcgcg
    11101 gccagcggcg gcgcctgcac ggtcactcct cggtccagca tgtggcgaat ccgctgctcg
    11161 ctcaacccga tttcgcgcag cacctcgagg ctgtgctcgc cgagcctggg cggcagccgg
    11221 cgaatcgccc ccggggtgtc cgagaagcgg gtggcgatgc cgggcagccg caaggtgccg
    11281 agctcggggt cctcgcgcat ctcccagaag ccgaccgcgg cgagatgcgg atcgtcgggc
    11341 aggtcgttgg gcgcattgac caccgtgcac ggcacgttga cctcgtcgag cgccgccagc
    11401 cactcggcgc tggtgcgccc ggccaggcag gcggcgatct cggcatagag cgcatcgacg
    11461 ttggcgagcc gcgcgccata gctcgcgaac cgcgcatcgt cgatcagctc cgggcggccg
    11521 gcgacgccga ggaacgcctg ccactggcgc tcggtatagg gcagcacgcc gatatagccg
    11581 tcggcggtcc ggtacggccg gcggtcgggc gacagcgcgc gcggataccc ggccggcccg
    11641 cgcgccggct cgaaggtgcg gccgtacagg tgctcgacca tgttgaacgc gaccagggtc
    11701 tcgaacatcg gcatctcgat cgcctggccg cggccgctgc gggcgcggct gaacagcgcc
    11761 atcacgatcg cctgcgccgc ggccatggcg cagatcttgt cggcgatgat cgaggtggcg
    11821 tagaccggcc gccccaggtt gcggccctgc agggcggcga agcccgaggc ggcctggatc
    11881 atgtcgtcga acgccggctt gtggctgtag ggcccgccag cgcggaagcc gtaggccgca
    11941 cagtagacga tggccgggct ggcggagctc acctcggcgt aggcgaggcc gaggcgcgcg
    12001 atcgcctgcg ggcgcatgtt gtgcaggaac acgtccgcgc gcgcggcgag cgcgagcagg
    12061 gcatcgcgcc cgtccgcctg cttgaggtcg agcaccagcg agcgcttgtt gcggttggag
    12121 ccgatgaaca gggtgccgcg gccccgggtc ggctcagggc cgctggtgcg cgcggtatcg
    12181 ccctcgggcg attcgacctt gatcacgtc
//
LOCUS       DASWHU010000311         8414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2417458, whole genome shotgun sequence.
ACCESSION   DASWHU010000311 DASWHU010000000
VERSION     DASWHU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417458"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGB88_11495"
     CDS             complement(<1..304)
                     /locus_tag="VGB88_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9648116.1"
                     /translation="MPRTVAQSHRQRNSDRSARSRRARAGLADVRLNDLRHSFAGVGA
                     AAGLDLPIVGRLLRHRDPKTTARYSHIGDDRARRRRPRVRHHRRRQVVQKRRRGCAV"
     gene            350..946
                     /locus_tag="VGB88_11500"
     CDS             350..946
                     /locus_tag="VGB88_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012325548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9648117.1"
                     /translation="MRHVKLAYLALAAGALLPLHADAQGLPGLRGTDHVAITVPNLTE
                     AVSFLVDVVGCEAFYVMGPFKSDDNWMKVHLDVDPRAEIPRMQLVRCGYGSNLEVIEY
                     TAPDQKTNVPRNSDIGGHHIAFYVDDIDAAVARLKAAGVRMLGEPTTNTAGPNAGTTW
                     GYFLAPWGLYLEIVSYNEGNAYEKDYDKRLWDPRQPGM"
     gene            1029..2309
                     /locus_tag="VGB88_11505"
     CDS             1029..2309
                     /locus_tag="VGB88_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX9648118.1"
                     /translation="MRRLTGRGRGRRWAATLGVLVLAAGWAAPAVAHLLATFNLRVVH
                     FEHHDDGLTAYFRLTLPLVVAGGLGPQRADGTFEPAPYTFNRLESGTVFHYVDVDQVR
                     RDVAGLGALVAAGHSLIVDDAPVAAEVVSARVHPRGQVPPFNTLAEVKAAVDGPPYPA
                     AAETILADFALVDVALRYRHPGGMSRFRFSSTLAVGVLGDARTVNMLVDHGPDRDVVY
                     RFDGFLHSAVTINPSPLSAGWSFTVEGIRHILEGRDHLLFIFCLTIGAATLGGLAWRV
                     TGFTAGHTVTLIGGFLGYAPDAAWFMPLVEAAIALSIIIAALAVWLPRLSATTTAATA
                     VIGLIHGFGFSYALQAILRTDAPNLLVSLGSFNVGVEIGQLAFILVAWPALLWLRTRT
                     PRFARDARAGIAVASIAIASVWLYERAPMIWQAA"
     gene            2405..2833
                     /locus_tag="VGB88_11510"
     CDS             2405..2833
                     /locus_tag="VGB88_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648119.1"
                     /translation="MRDTTVGSSGTRAPRGLVVALAAGAVLAAAQALCAGPALAQENY
                     NLAETHSAVYSTVYEDDSVWIWEAYYEPGEKGTPHSHAKPYTVYIVKGTTFRITEEDG
                     SVRDVELKVGAHFESPALKNHWGWNTDEQNDARFIIVQPK"
     gene            3037..3426
                     /locus_tag="VGB88_11515"
     CDS             3037..3426
                     /locus_tag="VGB88_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEX9648120.1"
                     /translation="MPNPAREHQQAPAATEATVLTKAALRAAARLGLTNKTLAEVIGV
                     SEATVSRMKDGAYLLRRGRKPFELAVLFVRLYRSLDAIVGGDERVAREWLRSRNTVLG
                     EVPGTLIRRVDGLMDVIQYLDARRAIV"
     gene            3476..4153
                     /locus_tag="VGB88_11520"
     CDS             3476..4153
                     /locus_tag="VGB88_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX9648121.1"
                     /translation="MVSTFKLVDDLDEQATLESLINQAKPPVPPDCRHLHYLLSTPFR
                     YGAPYPGGSRFRRAGQTEGVFYASSTVETAICEAAFWRLLFFAESPDTPWPSNPAAHT
                     AFSTDYASDDALDLTAAPLIADRADWVHPTDYAPCQDLADRARNVAIEVIRYESVRDP
                     RRRANIAIMRCAAFSTAEPTVSQTWRIHLGDRGVRAIGEFPPARIEFGRFESCPDPRL
                     AAIRWQR"
     gene            4339..5343
                     /gene="dctP"
                     /locus_tag="VGB88_11525"
     CDS             4339..5343
                     /gene="dctP"
                     /locus_tag="VGB88_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9648122.1"
                     /translation="MTGWTRILAGAGAGVLCALALGAQAGEVTLRASHQWPSGTGDVR
                     DEMVQLIAREVAAAGVGLELRIYAASSLFKPKQQWDAMVKDQLDISAFPLDYASGRHP
                     QFSATLMPGLVKNHEHARRLNDSPFMAEIKRIIDDAGVMVLSDAWLAGGFAGRERCVV
                     RPDDATGMVMRAAGPAFEQMLAAAGASINAMPSSEIYSALQTGVLDAVNTSSGSFVSY
                     RIYEQAKCLTPPGAYALWFMYEPILMSRQSFARLDADQQRALVDAGRAAEDYFFEAAK
                     GLDTELVETFKAHGVEVAEMGKADFDAWLALARESAYRHFADEVPGGAELIDKALAVE
                     "
     gene            5380..5898
                     /locus_tag="VGB88_11530"
     CDS             5380..5898
                     /locus_tag="VGB88_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9648123.1"
                     /translation="MDAFIAAVRALSRACGVVSVLLLLAAVLVVNHLVILRYVLNASA
                     IWQYEFVTYSLIGATIIGSPYVLMTRGHVNVDLLPLYLGPRLRFALALIAAVTGLGFC
                     LTLAVTGIGFWYQAWSKGWVSETVWAPPLWIPYLAVPLGMGLLALQYLADILALVTGR
                     EPPFGLGDEGRR"
     gene            5895..7202
                     /locus_tag="VGB88_11535"
     CDS             5895..7202
                     /locus_tag="VGB88_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9648124.1"
                     /translation="MSELELGLLVAAVSIAVILTGAPVAFALGAVAVGFLVAHDGWGA
                     LSFVPDVFFGALDSFVLLSIPMFILMGGIVASSRAGADLYEALDRWLHRVPGGLMASN
                     LAACAIFAALSGSSPATCAAIGKMGIPEMRKRGYPAAVATGAIAAGGTLGILIPPSIT
                     MIVYGIATETSIGRLFVAGVVPGVMLTALFVAWGVFAARRRGYDPADPSVRYSLRQRI
                     EVLPKVAPFLAIIAAILYVLYGGIATPSEAAGVGALFCLVLVVVIYRLRRPRELWRIF
                     EQAMRESVMIMMIIAAADLFSYMMSSLFITQSLAMWVAALELDRWLLMVCINLFLLVA
                     GFFLPPVAVILMAAPTLLPIVQGLGFDPVWFAVVLTLNMEIGLITPPVGLNLYVINGI
                     APDVRLQTVLAGALPFMLLMVLAIVLLCVFPGIALWLPRQVMG"
     gene            7297..8370
                     /locus_tag="VGB88_11540"
     CDS             7297..8370
                     /locus_tag="VGB88_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9648125.1"
                     /translation="MRRRAFLKGVAAGAAAGALPAPALAGDKLSWKLVTCWPRNFPAL
                     GTGSQRLADSITAMSGGRLTVKVYGAGELVPAFEAFDAVREGIAEMCHDAPYYWIAKH
                     PATAFFCSVPGGLTAQEQASWIYFGGGQALWDELYADFGLTAFLAGSGGPNVGGWFRK
                     EINSVDDFKGLRMRIPGLGGELLNRMGATTVNLPGAEILPALQTGAIDAAEWVAPYND
                     LAFGFHKVAKYYYAPGVHEPAPAGALTVNRKAFEALPADLQEIVRRAAGDEALRMLAE
                     MTAGNGAALEVLIGKHGVQLRRFPPDVYRALMATAAEVVADTAAKDPFTARVYQNWSA
                     FRQKQMALAPLTELGYMTIRAAS"
BASE COUNT         1164 a         2948 c         2927 g         1375 t
ORIGIN      
        1 caacggcaca gcctcgccgc cgcttttgga cgacctgtct gcggcgatgg tgccggacac
       61 gcggtcggcg gcggcgcgcg cgatcgtcgc caatatggct gtatctcgcc gtggtcttgg
      121 ggtcgcggtg cctcaggagc ctgccgacga tcggcaggtc gaggccggca gcagcgccga
      181 cgccggcgaa gctgtgcctg aggtcgttaa ggcgcacgtc ggcgagcccg gcacgggcgc
      241 gcctgctgcg tgccgatcgg tcggaattcc gctgccggtg cgattgtgct accgtcctcg
      301 gcattcgggc ggaaccgacc gggacaaacc cggctcaagg gaggagacca tgagacacgt
      361 caagctcgcg tatctcgcgc tcgcggcggg cgctctgctg ccgcttcacg ccgacgcgca
      421 ggggctgccg gggctgcgcg gcaccgacca cgtcgcgatc accgtgccca atctcaccga
      481 ggcggtaagc ttcttggtcg acgtcgtcgg ctgcgaggcc ttctacgtca tgggcccgtt
      541 caagtccgac gacaactgga tgaaggtcca cctcgacgtc gatccgcgcg ctgagatccc
      601 gcgcatgcag ctggtcaggt gcggctacgg ctcgaacctc gaggtgatcg agtacaccgc
      661 gcccgatcag aagacgaacg tgcccaggaa cagcgacatc ggcggccatc acatcgcctt
      721 ctacgtcgac gacatcgacg ccgcggtggc gcgcctgaag gccgccgggg tcaggatgct
      781 cggcgagccg accaccaaca ccgccgggcc caacgccggc accacctggg gctacttcct
      841 ggcgccgtgg gggctctatc tcgagatcgt gtcctacaac gagggcaacg cctacgagaa
      901 ggactacgac aagcgcctct gggatccgcg gcagccgggc atgtagggac cgcccgcgcg
      961 ggccctgacc gcgaggggtg gcgttccggt ccgggtgccg cccgcgtccg ctcggggcgg
     1021 cacacccgat gaggcgtctg accggccggg gccgcggtcg tcggtgggcg gcaacgctgg
     1081 gcgtgctggt cctggccgcg ggctgggccg cgccggcggt cgcccatctg ctcgccacct
     1141 tcaaccttcg ggtggtccat ttcgagcacc acgacgacgg cctgaccgcc tatttccgcc
     1201 tgaccctgcc gctggtcgtc gccggcgggc tcgggccgca gcgcgccgac ggcaccttcg
     1261 agccggcgcc gtacaccttc aaccgcctgg aaagcggcac ggtgttccat tacgtcgacg
     1321 tcgatcaggt gcgccgcgac gtggccggcc tcggcgcgct cgtcgccgcg ggccacagcc
     1381 tgatcgtcga cgacgcgccg gtcgcggccg aggtcgtctc ggcgcgggtg cacccgcgcg
     1441 ggcaggtgcc gcccttcaac accctggccg aagtcaaggc cgcggtcgac ggcccgcctt
     1501 accccgccgc tgcggagacc atcctcgccg acttcgccct ggtcgacgtg gcgctccgtt
     1561 accgccatcc gggcggcatg tcgcggttcc gcttttcgag caccctcgcc gtcggcgtcc
     1621 tcggcgacgc ccggaccgtc aacatgctcg tcgaccacgg cccggacagg gacgtggtct
     1681 accgcttcga cggattcctg cacagcgcgg tgaccatcaa tccctcgccc ttgagtgccg
     1741 gctggagctt caccgtcgag ggcatacgcc acatcctgga gggccgggac catcttctgt
     1801 tcatcttctg cctgaccatc ggcgccgcca cccttggcgg gctcgcctgg cgggtcaccg
     1861 gcttcaccgc cggccatacc gtgaccctga tcggcgggtt cctcggctat gcgccggatg
     1921 cggcctggtt catgccgctg gtggaggcgg cgatcgcgct gtcgatcatc attgccgctc
     1981 tggcggtgtg gttgccgcgc ctcagcgcga ccactaccgc ggcgaccgcg gtcatcggcc
     2041 tcatccacgg cttcggcttc tcatatgcgc tccaggcaat cctgcgcacc gacgccccca
     2101 acctgctggt cagcctgggc tcgttcaacg tcggcgtcga gatcggccag ctcgccttca
     2161 tcctggtggc gtggccggcg ctgctgtggt tgcggacgcg cacgccgcgc ttcgcccggg
     2221 acgcgcgcgc cgggatcgcg gtggccagca tcgccatcgc ttcggtctgg ctgtacgagc
     2281 gcgcgccgat gatctggcag gcggcctagg ggctctcttg ccggcgcccc tgtgcccgct
     2341 ctaggctcac ggccggcagc cgcaccgcgg cggcgataag gcgaaacggg aggaggagac
     2401 ccccatgcgt gacacgacag ttggctcgag cgggacccgc gcgccgcgcg gcttggtcgt
     2461 cgcgttggcc gccggcgccg tgctggcggc cgcccaggcg ctgtgcgccg ggccggcgct
     2521 ggcgcaggag aactacaacc tggcggagac ccattccgcc gtctactcga cggtctacga
     2581 ggacgactcg gtgtggatct gggaggccta ttacgagccc ggcgagaagg gcacgccgca
     2641 ctcgcacgcc aagccgtaca cggtctacat cgtcaagggc accacgttcc ggatcaccga
     2701 ggaggacggc agcgtccgcg acgtcgagct gaaggtgggc gcgcacttcg agtcgccggc
     2761 gctcaagaac cactggggct ggaacaccga cgagcagaac gacgcgcggt tcatcatcgt
     2821 ccagccgaaa taggctgcgg ccgggacccg tcgcgggcgc gccgattcgt ctcgacgacc
     2881 cggcagacgg gctttgcgcg gcgaggtgcc tgggacgccg tggggggcgg cgcgtcccca
     2941 actgcgagca ctcgcgatgc acattagtgt ggtcaaattc tcctcttgca tgagaaggtt
     3001 tacgagccta ttctttccat agagaaggaa tgcacggtgc caaaccccgc gcgcgaacac
     3061 cagcaggcgc cggcggccac cgaggccacc gtactgacca aggctgcgct gcgcgccgcg
     3121 gcgcgcctgg ggctgacaaa caagaccttg gccgaggtga tcggcgtctc agaggccacc
     3181 gtgtcgcgca tgaaggacgg cgcatacctg ctccggcggg gccgaaagcc gttcgagctg
     3241 gccgtcctgt tcgtgcggct gtatcgatcg ctcgacgcga tcgtaggcgg cgatgagcgc
     3301 gtcgcgcgag aatggctgcg gagcagaaat acggtgcttg gcgaagtgcc gggaacgttg
     3361 atccggaggg tcgacgggct gatggatgtc atccagtacc tggacgccag gcgcgctatc
     3421 gtctagccgc aagacgctgc gcggacaatg ctggcgcgtt gtcgaggcgc agcatgtggt
     3481 ctcgaccttc aagctggtcg acgatctaga tgagcaggcg accctcgagt ccctgatcaa
     3541 ccaggccaag ccacccgttc cgccggattg ccggcacctg cattatctcc tgtcgacgcc
     3601 gtttcgctac ggcgcaccct atcctggcgg ctcacgattt cgccgcgcgg gccaaaccga
     3661 gggggtgttc tacgcctcct ccaccgtcga gaccgcaatc tgtgaggcag cgttctggcg
     3721 gctgttgttt tttgccgagt cgcccgacac gccgtggccg tccaatccgg ccgcacacac
     3781 cgccttctcg acagactacg cgagcgacga cgcgcttgat ctgacggcgg cgcctttgat
     3841 cgcagacagg gcggattggg tgcacccgac cgactatgcg ccgtgccagg atcttgcgga
     3901 ccgcgcgcga aacgtagcga tcgaggtgat ccgatacgag tccgtgcgcg atccgcgcag
     3961 gcgggcaaac atcgcgatca tgaggtgcgc cgcattcagc accgccgaac cgaccgtctc
     4021 gcagacgtgg cgaatacacc tcggcgaccg cggtgtccgg gcgatcggcg agtttccgcc
     4081 agcgcgcatc gagttcggca ggttcgagtc ctgcccagac ccgcgtctgg cggccatccg
     4141 ctggcaacgc tagtggcagg atccatcgcc catctcccgc tgaacatgat cgaccgattg
     4201 agcagtccgc gggcgacggc gttcgtgtcc ggcgggacgc gtcgccggcg cctcggccgc
     4261 ggcgggcggt gggtcgcgca cgagtttccc tgctatgatc gccgggcgag gcggccatcg
     4321 gcggggagga gtcggcacat gacagggtgg accaggatac tggccggcgc gggtgccggc
     4381 gtcctctgcg cgctcgctct cggcgcccag gccggcgagg tcaccttgcg cgcctcgcac
     4441 cagtggccgt ccggcaccgg cgacgtgcgc gacgagatgg tccagctgat cgcccgcgag
     4501 gtcgccgccg cgggcgtcgg cttggagctg cgcatctacg ccgcctcgtc cttgttcaag
     4561 cccaagcagc agtgggacgc gatggtcaag gaccagctcg acatctcggc cttcccgctc
     4621 gattacgcga gcggccgcca cccccagttc agcgccacgc tgatgcccgg cctggtcaag
     4681 aaccacgagc acgccaggcg gctcaacgac tcgccgttca tggccgagat caagaggatc
     4741 atcgacgatg ccggggtgat ggtgctgtcg gacgcctggc tcgccggcgg cttcgccggg
     4801 cgcgagcgct gcgtggtgcg ccccgacgac gccacgggca tggtcatgcg cgccgccggc
     4861 ccggcgttcg agcagatgct ggcggcggcc ggcgcctcga tcaacgccat gccgagctcc
     4921 gagatctaca gcgcgctgca gaccggcgtg ctggatgccg tcaacaccag ctcgggcagc
     4981 ttcgtttcgt atcgcatcta tgagcaggcc aagtgcctga ccccgccggg cgcgtacgcg
     5041 ctgtggttca tgtacgagcc gatcctgatg tcgagacaga gcttcgcgcg cctcgacgcc
     5101 gaccagcagc gggcgctggt cgatgccgga cgcgcggccg aggactactt cttcgaggcg
     5161 gcgaagggcc tcgacaccga gctggtcgag accttcaagg cgcacggggt cgaggtcgcc
     5221 gagatgggca aggccgactt cgatgcctgg ctcgcgctcg cgcgcgagtc cgcctaccgg
     5281 catttcgccg acgaggtgcc gggcggcgcc gagctgatcg acaaggcgct ggcggtcgag
     5341 taggccgccg acgcgccgcc gcgcgcggga cgattcgcga tggacgcctt catcgccgcg
     5401 gtgcgcgccc tgtcgcgcgc ctgcggtgtg gtctcggtgc tgctgctgct cgccgcggtg
     5461 ctggtggtca accacctggt catcctgcgc tacgtgctca atgcgtcggc catctggcag
     5521 tacgagttcg tcacctacag cctgatcggt gcgaccatca tcggcagccc ctacgtgctg
     5581 atgacgcgcg gccacgtcaa cgtcgacctg ctgccgctct atctcgggcc caggctgcgc
     5641 ttcgcgctgg cgctcatcgc cgcggtcacg ggcctcggct tctgcctgac actcgccgtg
     5701 accggcatcg gcttctggta ccaggcctgg tcgaagggct gggtctcgga gacggtgtgg
     5761 gcgccgccgt tgtggatccc gtacctcgcg gtgccgctcg gcatggggct gctggcgctg
     5821 caataccttg ccgacatcct ggcgctggtc accggccgcg agccgccgtt cggactcggc
     5881 gacgaggggc ggcggtgagc gagctcgagc tcggcttgct ggtcgccgcg gtgtcgatcg
     5941 ccgtaatcct gaccggcgcg ccggtggcgt tcgcgctcgg cgcggtcgcg gttggcttcc
     6001 tggtcgcgca cgacggctgg ggcgcgctct cgttcgtgcc cgacgtgttt tttggcgcgc
     6061 tcgacagctt cgtgctgctg tcgatcccga tgttcatcct gatgggcggc atcgtcgcct
     6121 cctcgcgcgc cggcgccgac ctgtacgagg cgctcgaccg ctggctgcat cgcgtgccgg
     6181 gcgggctgat ggcctcgaac ctcgccgcct gcgcgatctt cgccgcgctg tcgggctcat
     6241 cgcccgcgac ctgcgcggcg atcggcaaga tgggcattcc cgagatgcgc aagcgcggct
     6301 acccggcggc ggtcgccacc ggcgcgatcg ccgccggcgg cacgctcggc atcctgatcc
     6361 cgccgagcat caccatgatc gtctacggca tcgccaccga gacctcgatc gggcggctgt
     6421 tcgtcgccgg cgtggtgccg ggggtgatgc tgaccgcgct gttcgtcgcc tggggcgtgt
     6481 tcgcggccag gcgccggggc tacgacccgg ccgacccgag cgtccgctac agcttgcgcc
     6541 agcgcatcga ggtgctgccg aaggtggcgc ccttcctcgc catcatcgcc gccatcctgt
     6601 acgtgctcta cggcggcatc gcgacgccgt ccgaggccgc cggggtcggc gcgctgttct
     6661 gcctggtgct ggtggtcgtc atctaccgcc tgcgcaggcc ccgcgagctg tggcgcatct
     6721 tcgagcaggc gatgcgcgaa tcggtgatga tcatgatgat catcgccgcc gccgacctgt
     6781 tcagctacat gatgtcgtcg ctgttcatca cccagtcgct cgccatgtgg gtggccgcgc
     6841 tcgagctcga ccgctggctg ctgatggtct gcatcaacct gttcctgctg gtcgcgggct
     6901 tcttcctgcc gccggtggcg gtgatcctga tggccgcgcc gacgttgctg ccgatcgtcc
     6961 agggcctcgg cttcgacccg gtgtggttcg cggtcgtgct caccctcaac atggagatcg
     7021 gcctgatcac gccccccgtg ggcctcaacc tctacgtcat caacggcatc gccccggacg
     7081 tgcgcctcca gaccgtgctc gccggcgcgc tgccgttcat gctgctgatg gtgctcgcga
     7141 tcgtgctcct gtgcgtcttc cccggcatcg cgctgtggct gccgcgccag gtcatgggct
     7201 aagggccacg gccgcggcgc gcaagcctct ggcctcgtgc cggcaaccgt ggtccgatgg
     7261 ctgggccaca tggggccaga cggggagggc cgggcgatga gacggcgtgc gtttctcaag
     7321 ggcgtcgcgg cgggggcggc ggcgggcgcg ctgcccgcgc cggcgctggc gggggacaag
     7381 ctgagctgga agctggtcac ctgctggccg cgcaacttcc cggcgctggg gaccgggtcg
     7441 cagcgcctgg ccgattcgat caccgccatg tcgggcggca ggctcacggt caaggtctac
     7501 ggcgcgggcg agctggtgcc ggcgttcgag gcgttcgacg cggtgcgcga gggcatcgcc
     7561 gagatgtgcc acgacgcgcc ctactattgg atcgccaagc acccggcgac ggctttcttc
     7621 tgctcggtgc cgggcgggct gaccgcccag gagcaggcgt cgtggatcta cttcggcggc
     7681 ggccaggcgc tgtgggacga gctctacgcc gacttcgggc tgacggcgtt cctggcaggc
     7741 agcggcgggc ccaatgtcgg cggctggttc cggaaggaga tcaactcggt cgacgacttc
     7801 aagggcttgc gcatgcgcat cccgggcctc ggcggcgagc ttctgaaccg catgggcgcg
     7861 accacggtga acctcccggg cgccgagatc ctgcccgcgc tccagaccgg cgcgatcgat
     7921 gccgccgagt gggtcgcgcc ctacaacgac ctcgccttcg gcttccacaa ggtggccaag
     7981 tactactacg cgccgggggt gcacgagccg gcgccggccg gggcgctcac ggtcaaccgc
     8041 aaggcgttcg aggcgctgcc cgccgacctc caggagatcg tccgccgggc ggcgggcgac
     8101 gaggcgctgc gcatgctggc cgagatgacc gccggcaacg gcgcggcgct cgaggtgttg
     8161 atcggcaagc acggggtcca gctccgccgc ttcccgcccg acgtctaccg ggcgctgatg
     8221 gcgaccgccg ccgaggtggt cgccgacacc gccgccaagg acccgtttac ggcgcgcgtg
     8281 taccagaact ggtcagcctt ccggcagaag cagatggcgc tcgcgccgct caccgagctc
     8341 ggctacatga ccatccgcgc cgcgtcgtga cgcgccgccc cccacctgcc tccccccgca
     8401 cgcgggggga ggtt
//
LOCUS       DASWHU010000312         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1282444, whole genome shotgun sequence.
ACCESSION   DASWHU010000312 DASWHU010000000
VERSION     DASWHU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282444"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGB88_11545"
     CDS             complement(<1..310)
                     /locus_tag="VGB88_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX9648126.1"
                     /translation="MNSFTKVLLAAGAAGVIGVVAIAGTSQAGRWGGGGHHGGFGAMR
                     MLDQFDANGDGKVTQDEIDTARSARFAKYDANGDGKLGIDEFDALFREVTRPMMVRAF
                     Q"
     gene            480..1199
                     /locus_tag="VGB88_11550"
     CDS             480..1199
                     /locus_tag="VGB88_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9648127.1"
                     /translation="MERTPHILVVDDHRDIRDLLARYLARHELRVTAAGDAAEARRAL
                     KGASIDLVVLDIMMPGEDGLSLCRYLRESAGLPVILLTAMAEETDRVVGLEIGADDYV
                     TKPFAPRELLARIRAVLRRTHSLPPSREPAGHGKVRFDRWTLDLDRRELVGEDGVLTP
                     LSTGEFRLLAAFVEHPRRVLSRDQLLDLTRGRAAAPFDRSVDNQVSRLRRKLERDARK
                     PLLIKTVWGGGYSFAAEVTRA"
     gene            1196..>2362
                     /locus_tag="VGB88_11555"
     CDS             1196..>2362
                     /locus_tag="VGB88_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9648128.1"
                     /translation="MTLVPRSFAGRLALLLVLALLVAQAAAFALFAHERAQALRHVYR
                     DAVVARTASVVRLIRDTQPATHLRVAEAASTRWLRYRLADRPDIAGGAAEGRAADIAG
                     DLARRLELAPDQVRADFALLRARADERAEDDRDDEDELRPERSRAGGAARLRWLRLSV
                     ALPDGRWLNVSAGAAPGAPRLGGPFLVTLAVSALAVAGVAVLAARRLSRPLLRLSHAA
                     DRLGRGEAVDSLPEEGPEEVRRSVRAFNRMRERLDRFVRDRTAMLAAISHDLRTPITS
                     LRLRAEFVTDEDTRARILETLDEMQEMTEAALAFAREEATAEQTRSVDLAALIDSVAA
                     DLAELGYAVDFAGAARTPIPCRPGALRRALRNLIDNAATYGRRARVSLDGDAAEL"
BASE COUNT          284 a          895 c          864 g          319 t
ORIGIN      
        1 tctggaaggc ccgcaccatc atcggccgcg tgacctcgcg gaacagggca tcgaactcgt
       61 cgatgccgag cttgccgtcg ccgttggcgt cgtacttggc gaaccgcgcg ctgcgcgccg
      121 tgtcgatctc gtcctgggtg accttgccgt cgccgttggc gtcgaactgg tcgagcatgc
      181 gcatggcgcc gaacccgccg tggtggccgc cgccgcccca gcgcccggcc tggctggtgc
      241 cggcgatggc gaccacgccg atcacgcccg cggcgccggc cgcgagaagg accttggtga
      301 agctgttcat gacttggctc cggttccggt ttcgggatgc cgccaatatg ggcgggacct
      361 gtcgcaacac tatgccggga cggggcgatc ttgtcgcagc cttgcgcggt cccggccgct
      421 gacacacctt gcgacaaaat agtcgcgcac ccgcccggtg aacgggctag ctgttggata
      481 tggagcgcac gccgcacatc ctggtcgtcg acgaccacag ggacatccgc gacctgctgg
      541 cgcgctacct ggcgcgccac gagctgcgcg tgaccgcggc cggcgacgcg gccgaggcgc
      601 ggcgggcgct gaaaggggcg tcgatcgacc tggtggtgct cgacatcatg atgcccggcg
      661 aggacggcct gtcgctatgc cgctacctgc gcgagagcgc cgggctgccg gtgatcctgc
      721 tcaccgcgat ggccgaggag accgaccgcg tggtcgggct ggagatcggc gccgacgact
      781 acgtgaccaa gccgttcgcc ccgcgcgagc tgctggcgcg gatcagggcg gtgctgcgcc
      841 ggacccacag cctgccgccc agccgcgagc cggcgggcca cggcaaggtg cgcttcgacc
      901 gctggacgct cgacctcgac cgccgcgagc tggtcggcga ggacggcgtg ctgacgccgc
      961 tgtcgaccgg cgagttccgc ctgctggccg cgttcgtcga gcacccgcgc cgggtgctgt
     1021 cgcgcgacca gcttttggac ctgacccgcg gccgcgccgc ggcgccgttc gaccgcagcg
     1081 tcgacaacca ggtcagccgg ttgcgccgca agctcgagcg cgacgcgcgc aagccgttgc
     1141 tgatcaagac cgtgtggggc ggcggctaca gcttcgccgc cgaggtgacg cgggcgtgac
     1201 cctggtgccg cggagcttcg ccgggcggct cgcgctcttg ctggtgctcg cgctcctggt
     1261 cgcccaggcc gccgccttcg ccctgttcgc ccacgagcgc gcccaggcgc ttcgccacgt
     1321 ctatcgcgac gccgtggtcg cgcgcaccgc aagcgtcgtg cgcctgatcc gggacaccca
     1381 gccggccacc catctccggg tggccgaggc cgccagcacg cgctggctgc gctaccggct
     1441 cgcggaccgc cccgacatcg ccggcggcgc ggccgagggt cgggccgcgg acatcgccgg
     1501 cgacctcgcc cgcaggctgg agctggcgcc cgaccaggtg cgcgccgact tcgcgctgct
     1561 gcgcgcgcgc gccgacgagc gcgccgagga cgaccgcgac gacgaggacg agctcaggcc
     1621 cgagcgctcg cgcgccggcg gcgcggcgcg gttgcgctgg ctgcgcctgt cggtcgcctt
     1681 gcccgacggg cgctggctca acgtgtccgc gggcgcggcg cccggcgcgc cccggctcgg
     1741 cgggccgttc ctggtcaccc tggcggtctc ggcgctggcg gtcgcgggcg tcgcggtgct
     1801 cgcggcccgg cggctgtcgc ggccgcttct gcggctgtcg cacgccgccg accggctcgg
     1861 gcgcggcgag gcggtcgatt cgctgcccga ggaggggccc gaggaggtgc gccgcagcgt
     1921 ccgcgccttc aaccgcatgc gcgagcgcct cgaccgcttc gtgcgcgacc gcaccgccat
     1981 gctcgccgcc atctcgcacg acctgcgcac gccgatcacg agcttgcgcc tgcgcgccga
     2041 gttcgtcacc gacgaggaca cgcgcgcgcg catccttgag accctcgacg agatgcagga
     2101 gatgaccgag gcggcgctcg ccttcgcccg cgaggaggcg accgccgagc agacgcgcag
     2161 cgtcgatctc gccgcgctca tcgacagcgt cgccgccgac cttgccgagc tcggctacgc
     2221 ggtcgacttc gccggcgccg cgcgcacccc gatcccgtgc cggccgggcg cgctcaggcg
     2281 cgcgctgcgc aacctgatcg acaacgccgc gacctacggc cggcgcgcgc gggtaagcct
     2341 cgacggcgac gcggcggagc tt
//
LOCUS       DASWHU010000313         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_561021, whole genome shotgun sequence.
ACCESSION   DASWHU010000313 DASWHU010000000
VERSION     DASWHU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561021"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..244)
                     /locus_tag="VGB88_11560"
     tRNA            complement(171..244)
                     /locus_tag="VGB88_11560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Cys,seq:gca)
     gene            458..1129
                     /locus_tag="VGB88_11565"
     CDS             458..1129
                     /locus_tag="VGB88_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX9648129.1"
                     /translation="MPDFDTMRAHMVDSQILPNKVTDPRLIEALRAVPRELFVPEAKR
                     GVAYVDEDLPLGGGRYLMEPLVFARLVEAAAVAAADTVLDLGCASGYSTAVLGRLAAS
                     VIGVESDPDLLGMAGAVLARLEVDNAVVVEGALAEGFPEQAPYDVILVNGAVATLPQS
                     ITAQLAEGGRLVTVIVDRQVSDRCHVGQAVRLTRHGGMLSRRPLFDAAVPPLPGFEVQ
                     PGFVF"
     gene            1220..2635
                     /locus_tag="VGB88_11570"
     CDS             1220..2635
                     /locus_tag="VGB88_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX9648130.1"
                     /translation="MTSARRFAPHGALLAVAALIAASAPAAAQSLDQALAQGYATNPR
                     LLAERAALRAVDEGVPQALSGWRPTVEVGANIGKRYLNNKPEGFPSSSSTTVPYQTGL
                     TLSQPIYRGGGTVAGTRAAEARVLAGRANLIDVEQQVLVDVTTAYMSVVRDLSVVDLN
                     ISNEQVLERQLQAARDRFEVGEVTRTDVSQAEARLSDAQALRIEAEGNLEVSRANFLR
                     AVGILPETLTFPDLSTMLSLPESRQAALAVAEQRNPALLRAGYLERSALNNISVAGAQ
                     LLPQVSIDGTVARSWEPSSFFERSDTAAVFANVTVPLYQSGAEYSRVRELRQIAVQRR
                     REVDDALRAVRAAVEQSWETLQSLRARIRAFESSVRANEIALDGVQQEAAVGARTVLD
                     VLDAEQELFLARVNLVRAESEEVVISFQLLQVMGGMTVIDLALPVEFYDPAAHYDEVR
                     EKWIGFGSDYQGGNPFGIGPE"
     gene            2765..3367
                     /locus_tag="VGB88_11575"
     CDS             2765..3367
                     /locus_tag="VGB88_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX9648131.1"
                     /translation="MAVTEDKAQHEPSMEEILSSIRRIISADGEDEPAADAANGRGAG
                     HAPEARTARAPAKPVADDAETEVEDTLAAEDDDEEEVLELTDPAEPEPEPEPQPVEDE
                     APAAAPARPAPVRDAPVRDAPGLVSPGTAAASVTSLAQLADKLAATSPPEAFAGRTVE
                     QLVVDLLRPMLREWLDQNLPGMVDTLVRAEIERLAGLVRR"
     gene            3455..>6085
                     /locus_tag="VGB88_11580"
     CDS             3455..>6085
                     /locus_tag="VGB88_11580"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9648132.1"
                     /translation="MLDKTYRPEQVEQRIYARWLEARAFDADPADSRPPFAVMMPPPN
                     VTGSLHMGHALNMTLQDVLTRYWRMRGRNALWQPGTDHAGIATQMVVERQLADQGLDR
                     RALGREKFVERVWQWTEQSGGMIARQLRRLGASPDWRRERFTLDEGLSRAVRTVFVRL
                     YRDGLIYRDKRLVNWDPELHTAISDLEVDQREVKGHLWHLRYPIEGADGAFITVATTR
                     PETMLGDTAVAVHPEDPRYRHLIGRHALLPLVGRRIPIIADEYANPEQGSGAVKITPA
                     HDFNDFEVGRRHGLEAINVLDARGRLNDAVPEAYRGLDRFAARARVVADMDALGLLEK
                     VEDQVHAVPYGDRSNAPIEPWLTDQWYVDAGRLAGPAIAAVEEGRIRFVPANWANTYF
                     EWMRNIQPWCISRQLWWGHQIPAWYGPDGKVFVAIDEAEAEELARRHYGRASELRRDE
                     DVLDTWFSSGLWPFSTLGWPEKTPELATFYPGDVLVTGFDIIFFWVARMIMLGLYCMG
                     KVPFRDVYIHALVRDEHGQKMSKSKGNIIDPLVLMDSYGTDALRFTLAAMAAQGRDIK
                     LSEQRVSGYRNFVTKLWNAARFCQMNECRPDPSFDPAACRSTTNRWIVGELAGAVHQA
                     GEAIEAYRFNDAAGALYQFLWHTFCDWYLEISKPVLYGDDEAAKAETRAAAAWTLDRA
                     LAALHPLMPFVTEELYASLAERGEAMLITAAWPDAAGMPVDQAAREELDWVVRLVSEI
                     RSVRAEMNVPPAAELPVALAEAGELSRQRFARYSGTIETLARVKTQRLDGGGAAGARA
                     GAVQLVFEDASLLLWVADLVDVAAERARLARELKKVISEIAKLDTKLSNEQFLARAPE
                     DVIAEQRERRAEADLSRRKLD"
BASE COUNT          897 a         2110 c         2199 g          879 t
ORIGIN      
        1 atcgtcccgg cctgcctcga agcgccgtac cggcgttcgg cgcaagcggt ggccctgcgc
       61 ggcggtcggg ccggggcacg cgaggctcct gcgggaacgg tcctctgcga cgcgagccaa
      121 gccgggagcg gggcgcgcag cccgtaagta acgagtacct tagggaaatc tggaggccgg
      181 gaccggaatc gaaccgacgt acgcggattt gcagtccgct gcataaccac tctgccaccc
      241 ggccgccccg cgcgcgctcc gcgacgccgc tgcgggcttg atataggccg ggccggcgaa
      301 ggcggtcaag ccatcgccgg gcaggtgcca tgcgcccgcc ggcgttgccc ggatgtcaat
      361 cccattctat atctccccgc ctatccgggc gcgctcgggc cccgccgggg cggggcaggg
      421 cgcgggtcac gacgccggag gagcggggct ttacctcatg ccggacttcg acaccatgcg
      481 cgcgcacatg gtcgacagcc agatcctgcc caacaaggtc accgacccgc ggctgatcga
      541 ggccctgcgg gcggtgccgc gcgagctgtt cgtgcccgag gccaagcgcg gcgtcgccta
      601 tgtcgacgag gacctgccgc tcggcggtgg ccgctacctg atggagccgc tggtgttcgc
      661 gcgcctggtc gaggcggccg cggtggccgc cgcggacacg gtcctcgacc tcggctgcgc
      721 cagcggctac tcgaccgccg tgctggggcg gctggcggcg agcgtcatcg gcgtcgaatc
      781 ggaccccgac ctgctgggca tggcgggcgc ggtgctcgcc cggctcgagg tcgacaacgc
      841 ggtcgtggtc gagggggcgc tcgccgaggg ctttcccgag caggcgccct acgacgtgat
      901 cctggtcaac ggcgcggtcg cgacgctgcc ccagtcgatc accgcccagc ttgccgaggg
      961 cggcaggctg gtgaccgtga tcgtcgaccg ccaggtgtcg gaccggtgcc atgtgggcca
     1021 ggcggtgcgc ctgacccggc acggcggcat gctctcgcga cggccgctgt tcgacgccgc
     1081 ggtgccgccg ctgccgggct tcgaggtgca gccggggttc gtgttctagg ggagtcgcgt
     1141 aaagccggcg cgagtcgaca aacggtatca actcgggcgc ggccttggta atgtccgccg
     1201 ctgtggagga attggccaga tgacttcagc acgtcgcttc gcgccccacg gcgccctgct
     1261 ggcggtggcg gccctgatcg ccgcgtccgc gcccgccgcg gcccagagcc tcgaccaggc
     1321 gctggcccag ggctatgcca ccaatccgcg cctgctggcg gagcgcgccg cgctgcgcgc
     1381 ggtcgacgag ggcgtgccgc aggcgttgtc gggctggcgg ccgacggtcg aggtcggcgc
     1441 gaatatcggc aagcgctacc tcaacaacaa acccgagggc ttcccgtcgt cgagctcgac
     1501 cacggtacct taccaaaccg ggcttacgct gtcgcagccg atctaccgcg gcggcgggac
     1561 cgtggccggg acacgggccg ccgaggcccg ggtgctggcc gggcgggcca acctgatcga
     1621 cgtcgagcag caggtgctgg tggacgtgac caccgcctat atgagcgtgg tgcgcgacct
     1681 ctcggtggtc gacctcaaca tcagcaacga gcaggtgctg gagcgccagc tgcaggcggc
     1741 ccgcgaccgg ttcgaggtcg gcgaggtgac gcggaccgac gtctcgcagg ccgaggcgcg
     1801 cctgtccgac gcccaggcgc tgcgcatcga ggccgagggc aatctcgagg tatcgcgcgc
     1861 caatttcctg cgcgcggtcg gcatcttgcc tgagacgctg acgttcccgg acctgtcgac
     1921 catgctgtcg ctgccggaga gccggcaagc ggcgctggcg gtggccgagc agcgcaatcc
     1981 ggcgctgctg cgcgccggct atctcgagcg cagcgcgctg aacaatatca gcgtcgccgg
     2041 ggcgcagctg ctgccccagg tctcgatcga cggcaccgtg gcgcggagct gggagccgag
     2101 ctcgttcttc gagcgcagcg acaccgcggc cgtgttcgcc aatgtcacgg tgccgctcta
     2161 ccagtcgggg gccgagtact cccgggtccg cgagctgcgc cagatcgcgg tccagcgccg
     2221 gcgcgaggtc gacgatgcgc tgcgcgcggt gcgcgcggcg gtcgagcagt cgtgggaaac
     2281 gctgcagtcc ttgcgggcgc gcatccgcgc cttcgaatcg tcggtgcgcg ccaacgagat
     2341 cgcgctcgat ggcgtccagc aggaggctgc ggtcggcgcg cgcacggtgc tcgacgtgtt
     2401 ggacgccgag caggagctgt tcttggcgcg ggtcaacctg gtgcgtgccg aatctgaaga
     2461 ggtggtgatc tcgttccagc tgctccaggt gatgggcgga atgaccgtga tcgatctcgc
     2521 cttgccggtg gagttctacg acccggctgc ccactacgac gaggtgcgcg agaagtggat
     2581 cgggttcggc agcgactatc agggcggcaa cccgttcggt atcggccccg aataggcgcg
     2641 ccggcgccgt taagccggtc ttaaccttag gcgccaatgc tgccatctgg gcgggtggtc
     2701 atgtcgctgg tcatgatcct gcgcttgcac taagcttgtc gccacagacg aaaaggaacg
     2761 cgtcatggcg gtgacggaag acaaagccca gcacgagccg tccatggagg agatcctgtc
     2821 gtcgatccga cggatcatct ccgctgacgg cgaggacgag ccggcggccg atgcggcgaa
     2881 cggcagaggg gcgggacacg cgcccgaggc ccgcaccgcg cgcgcccccg ccaagcccgt
     2941 cgcggacgac gccgagacgg aggtcgagga cacgctggcg gccgaggacg acgacgagga
     3001 ggaggtcctg gagctgaccg acccggccga gcccgagccc gagcccgagc cgcaaccggt
     3061 cgaggacgag gcaccggctg ccgctccggc ccggcctgcg ccggtgaggg acgcgccggt
     3121 gagagacgcg ccggggctgg tctcgccggg gaccgcggcg gcgtcggtca cctcgctcgc
     3181 ccagcttgcc gataagctgg ccgcgacgag cccgccggag gccttcgccg gccgcacggt
     3241 cgagcagctc gtcgtcgacc tgctgcgccc gatgctgcgc gagtggctcg accagaacct
     3301 gcccggcatg gtcgacaccc tggttcgcgc cgagatcgag cggctcgccg gactggtcag
     3361 gcgctaggct cgccccgggc gcgcgcccgc cgcacgtcgc cggcccgtcg gccgcggcgg
     3421 aaggtccggg gcgcccgact tctctctgat cgtcgtgctc gacaagacct atcgacccga
     3481 acaggttgag cagcgcatct acgcacgctg gctggaggcg cgcgccttcg acgccgaccc
     3541 ggcggattcg cgcccgccgt tcgcggtgat gatgccgccg ccgaacgtca ccggcagcct
     3601 gcacatgggc catgccctca acatgaccct gcaggacgtg ctcacccgct actggcggat
     3661 gcgcgggcgc aacgcgctgt ggcagccggg caccgatcac gccggaatcg ccacccagat
     3721 ggtggtcgag cgccagctcg ccgaccaggg cctcgaccgt cgcgcgctgg ggcgcgagaa
     3781 gttcgtcgag cgggtctggc agtggacgga gcagtcgggc ggcatgatcg cccgccagtt
     3841 gcgccggctc ggcgcctcgc ccgactggcg gcgcgagcgc ttcactctcg acgagggcct
     3901 gagccgggcg gtgcgcaccg tgttcgtgcg cctctatcgc gacggcctga tctaccgtga
     3961 caagcgcctg gtcaactggg acccagagct gcacaccgcc atctccgacc tcgaggtcga
     4021 ccagcgcgag gtgaaaggcc atctctggca cctgcgctac ccgatcgagg gtgcggacgg
     4081 cgcgttcatc accgtcgcga ccacccggcc cgagaccatg ctcggcgaca ccgcggtcgc
     4141 cgtccatccc gaggatccgc gctaccgcca tctgatcggc cgccacgcgc tgctgccact
     4201 ggtcgggcgg cgcatcccga tcatcgccga tgagtatgcc aaccccgaac agggatcggg
     4261 cgcggtcaag atcacgcccg cccacgactt caacgacttt gaagtcggcc gccgccacgg
     4321 cctcgaggcg atcaacgtgc tcgacgcccg cggccggctc aacgacgccg tgcccgaggc
     4381 gtatcgcggg ctcgaccggt tcgcggcacg cgcgcgcgtg gtcgcggaca tggacgccct
     4441 ggggctgctc gagaaggtcg aggaccaggt gcatgccgtc ccctatggcg accgctcgaa
     4501 cgccccgatc gagccgtggc tgaccgacca gtggtacgtc gatgcgggcc ggctcgccgg
     4561 gcctgcgatc gcggcggtcg aggaggggcg catccgcttc gtgccggcga attgggccaa
     4621 tacctatttc gagtggatgc gcaacattca gccctggtgc atctcgcgcc agctgtggtg
     4681 ggggcaccag atcccggcct ggtacggtcc cgacggcaag gtgttcgtgg cgatcgacga
     4741 ggccgaggcc gaggagctcg cgcggcggca ttacggccgg gcgagcgagc tgaggcgcga
     4801 cgaggacgtg ctcgacacct ggttctcgtc cgggctgtgg ccgttctcga ccctcggctg
     4861 gcccgagaag acccccgagc tcgcgacctt ctatcccggc gacgtgctgg tcaccggctt
     4921 cgacatcatc ttcttctggg ttgcgcgcat gatcatgctc ggcctgtact gcatgggcaa
     4981 ggtgccgttc cgcgacgtct acatccacgc cctggtgcgc gacgagcacg gccagaagat
     5041 gtcgaagtcg aagggcaaca tcatcgaccc gctggtgctg atggacagct acggcaccga
     5101 cgcgctgcgc tttaccctgg ccgcgatggc cgcccagggg cgcgacataa agctgtccga
     5161 gcaacgggtg tccggctatc ggaacttcgt caccaagctg tggaacgcgg cccggttctg
     5221 ccagatgaac gagtgccggc ccgacccgag cttcgatccc gcggcgtgcc gcagcacgac
     5281 caaccgctgg atcgtgggcg agctcgccgg cgcggtgcac caggcgggcg aggcgatcga
     5341 ggcctaccgt ttcaacgatg cggccggcgc gctgtaccag ttcctctggc acaccttctg
     5401 cgactggtac ctggagatct cgaagccggt gctttacggc gacgacgagg ccgccaaggc
     5461 cgagacccgc gccgccgccg cctggacgct cgaccgcgcg ctcgccgcgc tgcacccgtt
     5521 gatgccgttc gtcaccgagg agctgtatgc gagcctcgcc gagcgcggcg aggcgatgct
     5581 gatcaccgcc gcctggcccg atgcggcagg gatgccggtc gatcaggcgg cgcgcgagga
     5641 gctcgactgg gtggtccggc tggtgagcga gattcgctcg gtgcgtgcgg agatgaacgt
     5701 gccgccggcg gccgagctgc cggtggcgct ggccgaagcg ggggagctct cccggcagcg
     5761 cttcgcgcgc tacagcggca ccatcgagac cctggcccgg gtcaagacgc agcgcctcga
     5821 cggcggcggt gcggcgggtg cgcgcgcggg cgcggtccag ctggtgttcg aggacgcgag
     5881 cctgttgctc tgggtcgcgg acctggtcga cgtcgccgcc gagcgcgcgc ggctcgcgcg
     5941 cgaactcaag aaagtcatct cggagatcgc caagctggat accaagctgt cgaacgagca
     6001 gttcctcgcc cgcgcgcccg aggacgtgat cgcagagcag cgcgagcgcc gcgccgaggc
     6061 cgacctgtcg cgacgcaagc tcgat
//
LOCUS       DASWHU010000314         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_919658, whole genome shotgun sequence.
ACCESSION   DASWHU010000314 DASWHU010000000
VERSION     DASWHU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919658"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGB88_11585"
     CDS             complement(<1..181)
                     /locus_tag="VGB88_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648133.1"
                     /translation="MTGPAPAARAERPVWVVRARTPLPCSPGERRPGQAMSVTAPQTD
                     AAPAIAGEDMEARVLA"
     gene            complement(178..1359)
                     /locus_tag="VGB88_11590"
     CDS             complement(178..1359)
                     /locus_tag="VGB88_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648134.1"
                     /translation="MAEPGNNPTRMELPKGAVPSPPDIPLKLLLVQFWRDIFGKEVQS
                     AATYSYLWIADQMGHVAVGIVCQFAMAFALGHVLAPLLDIGWLATWANGLALAAVAGA
                     VSFWEYRAFTVAKAGASSGLFPLDEDILRRNAIIAAAYMIFGVVVGYGFQLSAYWGAL
                     VFLVMVGAAMVCAPPWLRQKITWQKASLPYLFRLADLSVDIPKPLAGEVQRFIDTPPA
                     TGAPRQMIIVGPVASGRTPMVCGLGTELAFKKRKVRYLSFDTLIEISDQYRTRPPDAA
                     LPPIGPWGPKNIFYWPWFEAETLIIDDISPIIGAAMREFGPDTFEKILTTGLAGAAAE
                     LRSRDTIWVFGVESADISFKLARPGEEIALRYARNIQAFCEAESPPLVVQLPPAETRM
                     E"
     gene            1645..2373
                     /locus_tag="VGB88_11595"
     CDS             1645..2373
                     /locus_tag="VGB88_11595"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9648135.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEEAGADYVDVARESRRDGGGVP
                     AMMELLESEAVARPSFAPPFLKAGAQIIGQTANILLYLGARHRLAPAGEAGRLWTHQL
                     QLTVTDLVVEVHDTHHPISSGLYYEEQRAEARARTGHFLKARAPKYLGYFDRVLARNP
                     AGDKHLVGARLTYADLSLFQVVAGLRYAFPRTMARLETRMPRLVALHDRVAARPRLRA
                     YLESERRIPFNEDGIFRHYPELEA"
     gene            2449..>3188
                     /locus_tag="VGB88_11600"
     CDS             2449..>3188
                     /locus_tag="VGB88_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648136.1"
                     /translation="MPSLVAFDERTERLVRFVEDTAPADIVEATCRRLDDGEAPDRLL
                     EAAALAVSRSTVLPPSHHGGPVHPVAGLHAISGLRARLDGARARLPAIQGVALANKHI
                     HSPIMGPGAMAAIEPGALAGKTVPELLAGLESALAKRLAAAAERHLAALLGVAEPVQV
                     LEILLRVALPRNALDDHYFLYLVQAFRALDAVGWDHAEVILRPALCFLARHPMMEADP
                     DQRGRIIADGIDLYRNYTALERLVDDRGL"
BASE COUNT          463 a         1127 c         1135 g          463 t
ORIGIN      
        1 gcgcgagcac gcgcgcctcc atgtcctcgc ccgcgatggc gggcgcggcg tccgtctgcg
       61 gtgcggtcac cgacatggct tggcccggcc gtcgctcacc gggcgagcat ggcagaggcg
      121 tgcgcgcgcg cacaacccaa accggccgct cggcgcgcgc cgccggcgcc gggccggtca
      181 ctccatgcgg gtctcggccg gtggcagctg caccaccagc ggcggcgact ccgcctcgca
      241 gaacgcctga atgttgcggg cgtagcgcaa ggcgatctcc tcgcccggcc gcgccagctt
      301 gaacgatatg tcggcgcttt cgacgccgaa cacccagatg gtgtcgcgcg acctcagctc
      361 cgcggccgcc ccggcgagcc cggtggtgag gatcttctcg aaggtgtcgg ggccgaactc
      421 gcgcatcgcc gcgccgatga tcgggctgat gtcgtcgatg atcagggtct cggcctcgaa
      481 ccacggccaa tagaagatgt tcttggggcc ccacgggccg atcggcggca gcgccgcgtc
      541 gggcggccgg gtgcggtact ggtccgagat ctcgatcagc gtgtcgaacg acaggtagcg
      601 caccttgcgc ttcttgaacg cgagctcggt gccgaggccg cagaccatgg gcgtgcgccc
      661 cgaggcgacc gggccgacga tgatcatctg tcgcggcgcg ccggtggccg gcggggtgtc
      721 gatgaaccgc tggacctcgc cggcgagcgg tttgggaata tccacgctca ggtcggcgag
      781 gcggaacagg tagggcagcg acgccttctg ccaggtgatc ttctggcgca accacggcgg
      841 cgcgcacacc atcgccgcgc cgaccatcac caggaacacc agcgcgcccc agtaggcgga
      901 aagctggaag ccgtagccga ccaccacgcc gaagatcatg taggcggcgg cgatgatcgc
      961 gttgcggcgc aggatgtcct catcaagcgg gaacaggccg ctgctcgcgc cggccttcgc
     1021 caccgtaaag gctcggtact cccagaacga caccgcgccc gcgaccgcgg ccaaggcgag
     1081 cccgttggcc caggtcgcga gccaaccgat gtcgagcagc ggcgccagca cgtgcccgag
     1141 cgcgaacgcc atcgcgaact ggcagacgat gccgaccgcg acgtggccca tctggtcggc
     1201 gatccagaga taggaatagg tcgccgcgct ctgcacctcc ttgccgaaga tgtcgcgcca
     1261 gaactgcacc agcaaaagct tcagcgggat gtcgggcggc gacggcacgg cgcccttcgg
     1321 cagctccatc cgggtcggat tgttgccggg ctcggccacg gcgtcactgc cgctccgcgc
     1381 gacgcaagcc cggcgcggtg acgaaagcgg cggtagtatc gcaattattg gtgtcgcggc
     1441 gcatcgccac ccggcccctc ccgagcgcgg cagacgatag cacagccgcg catcgataga
     1501 aagcggcggc gtgcctgacg ccacgcgccc ggattgcgat tgaggcgcca ggcgcgcgga
     1561 gtcatactgc cggcgcttgt cttgatgcgc gttgggcggg tttcgcgcgt cccccggggc
     1621 aggccggcgg ggagggtcgg gtcggtgagg tacgagctct actattggcc gtcgatccag
     1681 gggcgcggcg agttcgtgcg cctcgcgctc gaggaggccg gcgccgacta cgtcgacgtc
     1741 gcgcgcgaat cacggcgcga cggcggcggc gtgccggcaa tgatggagct gctcgagtcc
     1801 gaggcggtcg cacgcccgtc gttcgcgccg ccgttcctca aggccggtgc ccagatcatc
     1861 ggccagaccg cgaacatcct gctctatctc ggcgcccgcc accgcctggc gccggcgggc
     1921 gaggcgggcc ggttgtggac ccatcagctg cagctcacgg tgaccgacct ggtggtcgag
     1981 gtccacgaca cccaccatcc gatctcgagc ggcctgtact acgaggagca gcgcgccgag
     2041 gcccgcgccc ggaccggcca tttcctcaag gcgcgggcgc cgaaatatct cggctatttc
     2101 gaccgggtgc tggcgcgcaa cccggccggg gacaagcacc tggtgggtgc gcgcctgact
     2161 tacgcggacc tgtcgctgtt ccaggtcgtg gccgggctgc gctacgcgtt cccgcgcacc
     2221 atggcacggc tcgagacgcg catgccgcgc ctggtcgccc ttcacgaccg ggtcgcggcg
     2281 cgtcccaggc tgcgcgccta cctcgagtcc gagcgccgca tcccgttcaa cgaggacggc
     2341 atcttccgcc actacccgga gctcgaggct taggcaatag catttgaggc ggggcggggc
     2401 gcggcgtaag gtcggcggcg ttcgctgccg cagggaaggg gtcgtgccgt gccgagcctg
     2461 gtcgcattcg atgagcggac ggagcgcctg gtccgcttcg tcgaggacac cgcgcccgcc
     2521 gacatcgtcg aggcgacctg tcggaggctc gacgacggcg aggcgccgga ccggctgctc
     2581 gaggccgcgg cgctcgcggt cagccgctca acggtgctgc ctccgtccca ccacggcggt
     2641 cccgtccatc cggtggctgg gctgcacgcg atcagcggtt tgcgggcgcg gctcgacggc
     2701 gcgcgcgccc gcctgcccgc gatccagggc gtcgcgctcg ccaacaagca catccattcg
     2761 cccatcatgg ggccgggcgc gatggcggcg atcgagcccg gcgcgctcgc cggcaagacc
     2821 gtgcccgagc tgctcgccgg gctcgaatcg gcgcttgcca agcggctggc ggccgctgcg
     2881 gagcgccacc tcgcggccct gctcggcgtc gccgagccgg tgcaggtgtt ggagatcctg
     2941 ctgcgcgtcg cgttgccgcg caacgccctc gacgaccact acttcctgta cctggtccag
     3001 gcgtttcgcg cgctcgacgc ggtcggctgg gaccatgccg aggtgatcct gcggccggcg
     3061 ctgtgcttcc tcgcccgcca cccgatgatg gaggccgatc cggaccagcg cggccggatc
     3121 attgccgacg gcattgattt gtatcgcaac tatacggcgc tagagcggct ggtcgacgac
     3181 cgcggcct
//
LOCUS       DASWHU010000315        21771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3156613, whole genome shotgun sequence.
ACCESSION   DASWHU010000315 DASWHU010000000
VERSION     DASWHU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 21771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21771
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3156613"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..877
                     /locus_tag="VGB88_11605"
     CDS             221..877
                     /locus_tag="VGB88_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX9648137.1"
                     /translation="MASRHAQAIAGRALFAGVAACCLAGRPSVAEALAALAGEPVYLV
                     PALMADGYTARTLLKRALTETAPADRARVVVRPPAGTHAGLARLCLGAALRACAVEGW
                     HADAARLVLAAHGTKRSARSAEAARRHAEAIAHGARFAHVSCGFLEQEPRLDAVLAEL
                     PAVPTVVVGLFADRGGHGEGDVGALVAAAPGPAHYAGPIGTDPGFAELIVARALAADA
                     "
     gene            complement(1076..2011)
                     /locus_tag="VGB88_11610"
     CDS             complement(1076..2011)
                     /locus_tag="VGB88_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9648138.1"
                     /translation="MITERIDAITLIPPEYRTAAPPCPKSVKIELTARCDFKCFFCAT
                     GDGLRTKGDIDWDLLRRLLAEMRQAGVDEIGMFYLGESFLYRRLPDAIAHASALGYPY
                     IFLTTNGRLARPDRVRACIEAGLDSLKFSFNWADPAQCREVTQVDAFDAVVANIAAAR
                     RVRDKVEAETGHRCGLYASSIQYDGGQRARMAAAVETIRPFVDEHYWLPLYNQAGLTA
                     GARGTRPVAGNVGRVGALRESLPCWALFTEGHITYDGRLSACCFDHDGRFDMGDLNRM
                     SFLEAWQGAPFRALRAANLQKDVAGTPCETCIAYQ"
     gene            complement(2200..2568)
                     /locus_tag="VGB88_11615"
     CDS             complement(2200..2568)
                     /locus_tag="VGB88_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648139.1"
                     /translation="MLALSALAGAPGALAGDAMMESKTAAQHAQLAADSADLEMVRAH
                     LHHAVNCLVGPDGDGFDSTQANPCQDQGSGAIPDAGDAAIKQKLEAALAVAMDGLASD
                     DLAQAQMAATKTGEMLNQGM"
     gene            2907..3251
                     /locus_tag="VGB88_11620"
     CDS             2907..3251
                     /locus_tag="VGB88_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9648140.1"
                     /translation="MIRSSLRRGLAALVLGAALAAPSAVADEPSFELVIRDHKFEPET
                     LEVPANARIKLIVQNADPTPEEFESYELNREKVIPGNSQAIVYIGPLDPGTYPFFGEF
                     HMDTAQGRIIAE"
     gene            3282..4853
                     /locus_tag="VGB88_11625"
     CDS             3282..4853
                     /locus_tag="VGB88_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX9648141.1"
                     /translation="MSATALIVFREVLEAALVVGIVMAATRGLPGRNAWIGAGLALGA
                     GGAVLVAAFAGAIAAAASGMGQELFNAAVLFIAVAMLGWHNVWMSRAGRAMARELTAV
                     GRAVRDAERPIYILAIVVGVAVLREGSEVVLFLYGIAAAQGTDAGAMLAGGAVGLALG
                     IAAGAVIYFGLIRLAGRYLFAITGGMILLLAAGMAAQGAKFLSQAGYLPTLGHGIWDT
                     SAVISDGGPLGNLLHVLIGYSARPDGIQLVFYIATLVAIGGLMYLFRTPKARVAAVAA
                     VAIVAAAATFGPTGGEPARAADLKVYSPVVEEGEFAIEARGNLTFDDAADKDRAQSQR
                     YEIEYTPNDFWHTALVGKLEQAAEGSLRYTATAWENILQLFPQGQQWLDLGVYLEYEL
                     ARDRSDADAFEGKILAEKNLGRVAFTVNPTFEQEIGGDDTDSTEFKYAARVKWRWKPE
                     LEPAIEAYGDIGEIGDVKAMRAQRHQVGPVLLGTFRLTDTSKLRYEAGYLFGLTSDGS
                     PDGAFKWLVEVEHYF"
     gene            complement(4844..6097)
                     /locus_tag="VGB88_11630"
     CDS             complement(4844..6097)
                     /locus_tag="VGB88_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX9648142.1"
                     /translation="MATGADQPSGREPGRVAGHAPLPRYRQAAVPAVLAHGFRPFFLL
                     AGIWAPVALALSVATIRGWITLPTAFDPIAWHYHEMLFGFVAATVTGFLLTAIPNWTG
                     RLPLQGGPLLALAGLWLAGRIAVAASALVGMWPAAVVDLAFLAAVAAVALREVVAGKN
                     WRNLPVVSAVLLFLACNLLSHGAVAGLVDDGLARRAAIAVIIALITLIGGRIIPSFSR
                     NWLVKRDPQALPASFGVVDRVSLAATALALAWWAATPVTATAAALAGLAAALNAVRLA
                     RWRGWRTRAEPLLWVLHVGYLWIPIGLALLAASFVVEGLSQTSAIHALTAGAMGTMTM
                     AVMSRATLGHTGRELRAGPGLSAAYVLVTVAALTRLIASAWTDAYMPLVITSATAWMV
                     AFALYLAVCGPLLARAQPPGAADQK"
     gene            complement(6292..6873)
                     /locus_tag="VGB88_11635"
     CDS             complement(6292..6873)
                     /locus_tag="VGB88_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648143.1"
                     /translation="MTGTRPRSNGACAMLCLLVGSLVTAAPAAADGSGTPAQVAATVP
                     AGPAEAEPEQRALDSGAVPLDRSRPEVCSEPWPRTFASLPAGHRDVALHLYLAQIAFL
                     AQTMQGGDRDAGGPGAVDIAHGYGPCPATGDIWSLEQIAVARLTSSGWGQVFHDMYRA
                     GLLDLGVLAGISGAVGPVFGTDLSPSGTASAGQ"
     gene            7213..7731
                     /locus_tag="VGB88_11640"
     CDS             7213..7731
                     /locus_tag="VGB88_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648144.1"
                     /translation="MLGGCAMASATREDVDRVSADEDHAPTAGGARPIVAVPETEPPA
                     SVLPDEAAGEQPAAIELSVRTPPAKPTPPPKPLPSPDPDKLLGMSTGAVTAELGAPAA
                     QRDEPPAHVWRYTTDFCSLDVFFFLDMTSDSRRVLAYDIQIIDGRSEDELLCYREIEK
                     RHAVASENIDLR"
     gene            7697..8467
                     /locus_tag="VGB88_11645"
     CDS             7697..8467
                     /locus_tag="VGB88_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9648145.1"
                     /translation="MLSQAKTSTSDNKRILVVDDDDLFRESIAANLSESGFDVSAFAD
                     GESFLDFMGEGCGDLVLLDWKMPGMSGIQVLHRIRELGIELPVIFLTHLSDQVYEERA
                     LGGGAVDFVEKTRSFTIIQRRIELILSGRRAGAAGIADAASDQKGVGVIRSASLTLDA
                     GSCRAFWNDLEVDLTLTEFEIVQWLVERAGRDVPYRDLYDLVHGPDFIAGLGSEGYRA
                     NVRAFIKRIRQKFRNLDDNFDGIVNYPGFGYRWRADGD"
     gene            8457..9998
                     /locus_tag="VGB88_11650"
     CDS             8457..9998
                     /locus_tag="VGB88_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9648146.1"
                     /translation="MATEAGAGRSGFSLLRRSISVKFLVLFLLFSIVPFIIYDQLQQA
                     YLQHRTLLLNSIQEQGRLVAIGIAPWLPSETPKELRATGKLLERYDSRQVSIKLLWRP
                     AATLEDSTLYYIGSAPAVPAAYLDEEVRRFREMGVISNVLGSCSGGGPVASRYVNPAG
                     GHEILTAITPILKGEECWIVITSASEEVLGEDGLGTPYWQRREVVLSGVIYLVVLAVT
                     LSLLLSVWGNVRRFGWLARRLRTARERSRRFVDLNRVPELAGVAQALDDLVSSLRRSA
                     ESLRESSEERAHAFKTPIATIAQSVQPIRNTLGDSDGRAGRALKVIERSLERLEELLV
                     ASHRMDEENAKLIDPPREPVDLAALVRQTASGYDQSLKAQDSAIEIRASVPDKAVIVE
                     ASEDLIETVLENLLDNAISYAPANGCVEVAVTASGGAARVTVADNGPGMDPAMLNRAF
                     ERYFSTRAGGRRGDDGGEAGAPSHFGIGLWVVRRNIEALGGRVALENRNGAGLVVRVD
                     IPLAR"
     gene            complement(10735..11298)
                     /locus_tag="VGB88_11655"
     CDS             complement(10735..11298)
                     /locus_tag="VGB88_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020581654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9648147.1"
                     /translation="MRIDAKPLASYPWYLRPFFWNQRRKYGAVLDSALLWARAPKLFL
                     GVAVLYGMIDRKSSPLDPALRSLVTVRVSQINWCRFCVDLNSATLLKRGVAPAKIEAL
                     DDWRASEQFEARERAALAYAEAITRTDQQVDEALFAELRHHFDDDTIIELTALIAFQN
                     LSSKFNSALDVPAQGFCRLPARSSRVG"
     gene            11472..12581
                     /locus_tag="VGB88_11660"
     CDS             11472..12581
                     /locus_tag="VGB88_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9648148.1"
                     /translation="MPAILTRDGLDALLAALRGRGYRVVGPTVRESAIVYDDIAATAD
                     LPEGWTDEQDGGRYRLVRRGDGALFGYAVGPQSWKKYLHPPILPLWRARRDGDGLSIE
                     ATEPVSERVAFIGVRACELHAIAIQDRVLLEGRYVDPHYRARREGAFIVAVNCGHAGG
                     TCFCVSMGTGPRAESGWDLALTELAGEGEHRFLIEAGTDAGREVLAALPHRAARPEEV
                     AAAEAVIAKTAANMGREMPAAEVKELLLANLEHPRWDQVADRCLTCGNCTMVCPTCFC
                     TSVEDSSDLDGAGAARTRRWDSCFTMDFSFIHGGSVRSSARSRYRQWMTHKLATWHDQ
                     FGSSGCVGCGRCITWCPVGIDITEEVHAIRGGSAR"
     gene            12593..13054
                     /locus_tag="VGB88_11665"
     CDS             12593..13054
                     /locus_tag="VGB88_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9648149.1"
                     /translation="MQGLERIVREHPFFAGLEEGFLELVCGCAKNVRFDAGDYLCHEG
                     EQANEFYMIRHGRVGLEITVPGRASVTFQTIGEGGIVGVSWLVPPYRWNYDAKALELV
                     RAIALDASCLREKSEQDHDLGYELMKRFMPILVQRLQATRLQILDVYGSPR"
     gene            13056..13904
                     /locus_tag="VGB88_11670"
     CDS             13056..13904
                     /locus_tag="VGB88_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX9648150.1"
                     /translation="MRALAPALGDAMVPRIARVRRRRRECPQTWTLEIEPEGAPPDVR
                     FAPGQFNMLYAFGVGEAPVSASGDPAAAGPLVHTIRAVGAVSTALVRLGPGDAVGLRG
                     PFGSGWPLAEAAGRDVVVIAGGLGLAPLRPLILRLLAERERYGKVALLYGTRSPLDIL
                     YRRELETWRRRLDSEIEVTVDHAAGGWRGNVGVVTDLVPRAAFDPLHTIAMVCGPEVM
                     MRFAIAALIEAGVAPDAIWLSMERNMKCAIGHCGHCQFGPAFVCKDGPVFRYDRVRDI
                     LALKEI"
     gene            13906..14688
                     /locus_tag="VGB88_11675"
     CDS             13906..14688
                     /locus_tag="VGB88_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9648151.1"
                     /translation="MARGARKPRLAVWKFASCDGCQLSLLDCEDELLAVAGALDIAYF
                     PEAMRGEVKGRYDLSLVEGSITTADDAERIKEVRRRSRRLVTIGACATSGGIQALRNF
                     KDVAAFISIVYARPDYIDSLATSTAIADHVPVDFELRGCPIDKRQLLEVIGAFLAGRK
                     PVTPAHSVCLECKLRGTVCVMVAHGTPCLGPVTHAGCGALCPAYDRGCYGCFGPMETP
                     NAPSLSGWLGALGMGARDLERVWRTFNANAPAFREESERHGA"
     gene            14678..15967
                     /locus_tag="VGB88_11680"
     CDS             14678..15967
                     /locus_tag="VGB88_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX9648152.1"
                     /translation="MARRTIQVKQLARVEGEGALKVTVRDGVVSSAELRIFEPPRFFE
                     AFLRGRAYREAPDITARICGICPVAYQMSAVHAMEDAFSVAVDAPLRDLRRLLYCGEW
                     IESHVLHVYMLHAPDFLGYDGAIDMARDHPDLVRRGLALKKAGNELMALLGGREIHPI
                     NVRVGGFYKTPRRRELAAVGERLKWARDAALETVTWVAGLDVPDRAYDYEFVALRHAR
                     DYPFNDGRIASSRGLDIAASEFDDHFEESHVKHSNALHARIRGRGAYLVGPLARYALN
                     FERLSPLAREAAKDAGLGPVCTNPYRSIVVRAVEVLYACDEALRLIEAYREPDRPAVP
                     VEPCAGTGHACTEAPRGILYHRYRLDADGTILEAHIVPPTSQNQASIEDDLRRYVAGW
                     LDLGDDELRHRCEQTVRNYDPCISCATHFLKLEMDRG"
     gene            15960..16442
                     /locus_tag="VGB88_11685"
     CDS             15960..16442
                     /locus_tag="VGB88_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX9648153.1"
                     /translation="MAERARRVVVGLGNPDRGDDGAGRTVARRLRGRLPRDVALVEHD
                     GEASGLLAQIEGADAAYLIDACASGAPAGTVRRFDAARAPLPEAAFEVSTHGLGLAAA
                     IELARALGALPRRCVVYAIEGQSFEVGAGLSPAAAAAVAEVVEQLVKEIRAPERAGHA
                     "
     gene            16435..16698
                     /locus_tag="VGB88_11690"
     CDS             16435..16698
                     /locus_tag="VGB88_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HEX9648154.1"
                     /translation="MHEAGLIAGLMRRILEVAEAENATRVVGASVWLGALSHMSAAHL
                     AEHFARAAAGTIAEGAALDIELSGDATHAEADGIVLHSVEVES"
     gene            16703..19048
                     /gene="hypF"
                     /locus_tag="VGB88_11695"
     CDS             16703..19048
                     /gene="hypF"
                     /locus_tag="VGB88_11695"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX9648155.1"
                     /translation="MSAPPPFELDMAERARLRIRVRGAVQGVGFRPFVYRRAAELGLA
                     GWVTNSTEGVTVEAEGDPGRLAALVESLRDAAPAPARVSQVETCAIPPCAAEGFEIRA
                     SVTTGARSAEVLPDLATCEDCLAELFDASDRRYLYPFINCTSCGPRYSIIEDLPYDRA
                     RTSMRRFEMCAACRSEYDNPADRRFHAEPNACADCGPSLALWDSSGAPVAHKHDALLA
                     AARMLGEGRIVAVKGLGGFHLMVDAQSEDAVRALRRRKRRPDKPFAVMFASLAEVATH
                     CRASAAEAALLVGPERPIVLVRRNSGAVADAVAPGNPWLGVLLAYAPLHHLLLGALGF
                     PVVATSGNLCDEPIVTDEHEAVRRLAGIADAFLVHDRAIVRPLDDSVVRVVAGRMLML
                     RRARGYAPAPVGEATLAPGILALGGHLKATVALTTASGAIVSQHIGDLETVEARAAYR
                     RAADDIARLHDARPRLVVRDLHPDYDSSRVAEAAGLPVVSVPHHLAHVAACLAEHGLD
                     APVLGVAWDGTGYGPDGTVWGGEFLHVTARAWRRVAHLATFRLPGGEAVAREPRRAAL
                     GALYRAFGPAALDMTDLAPVAAFEPAERRVLGAMLARGLNAPVTSSAGRLFDAVAALA
                     GVRQRASYEGQAAAELEWAAGEGANGARYDLALRPADGDGPLVVDWLPALAAIVADLR
                     AGIPAAIVSAKFHDGLAAAIAEVAAVIGEPRVVLSGGCFQNKRLTESTVAALRARGLE
                     PVWHRRVPPNDGGIALGQAAWAAWSESRGETPCAWRSPDES"
     gene            19018..19269
                     /locus_tag="VGB88_11700"
     CDS             19018..19269
                     /locus_tag="VGB88_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX9648156.1"
                     /translation="MCLAVPGRILSQSGDDPLMRVGRVDFGGVVKEINLAFVPEARIG
                     DHVLVHVGFAITVIDEAEAARVFEHLREIGELAEDGAAP"
     gene            19266..20384
                     /gene="hypD"
                     /locus_tag="VGB88_11705"
     CDS             19266..20384
                     /gene="hypD"
                     /locus_tag="VGB88_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX9648157.1"
                     /translation="MRFRDEYRAPAAAQGYARAIAKAATRPWTLMEVCGGQTHTIVRY
                     GIDQLLPVGIELVHGPGCPVCVTPVEYVDKAIEIASRPGATLCSFGDMLRVPGRGGDL
                     LGAKSRGADVRIVYSPLDALAVARAEPAREVVFFAVGFETTAPANAMAAWQARRQGLA
                     NFSLLVSHVLVPPAMRAILDAPANRIEGFLAAGHVCTIMGTREYEPIAARYRVPIVVT
                     GFEPLDILEGVYMCVRQLEDGRAAVENQYVRSVRRAGNLRAQALMGEVFRVIPRRWRG
                     IGDIADSGLGLAEAYADLDAERRFGAVRTVGEAAGECISGLVLQGARKPPECPAFGTR
                     CTPERPLGVTMVSSEGACAAYYRYRGAPRPAETESAGA"
     gene            20381..21433
                     /gene="hypE"
                     /locus_tag="VGB88_11710"
     CDS             20381..21433
                     /gene="hypE"
                     /locus_tag="VGB88_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX9648158.1"
                     /translation="MTAARGFAQSCPLPAGDGECVQLAHGGGGRAMARLLDALIRPAF
                     DDPELDRRHDGAALEVTGPVAFTTDSYVVKPLVFPGGDIGSLAVNGTVNDLAMCGARP
                     LYLSVGLIVEEGLALETLRAVVASIAAAAKAAGVRLVTGDTKVVDRGSADGLFVNTAG
                     IGEIVAPAPVGPAQVRAGDAVVLSGDIGRHGIAVMAAREALGFETAIESDCAPLAAPV
                     LALVESGIAVRCLRDLTRGGLASALVEIAETAGKRIAIEEAAIPVRADVSAACELLGL
                     DALHVANEGRFVAFVAAEDAARAVEVLRRYDAAGAAAVIGVVGDGPPGQVSCRGAIGA
                     SRVIDMLSGEQLPRIC"
     gene            21448..>21771
                     /locus_tag="VGB88_11715"
     CDS             21448..>21771
                     /locus_tag="VGB88_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX9648159.1"
                     /translation="MARAAPNRTATGLTPPPEPRRRWHAESIETVLAAVEATPEGLTQ
                     AAVEARLARHGPNRLTPPRPRGALARLLAQFHNVLIDVLLVAAAVTAGLGHWTDSAVI
                     AAVVVV"
BASE COUNT         3032 a         7752 c         7842 g         3145 t
ORIGIN      
        1 ctgctccagt ccgtgcttga gaaggccgtg caagcctcgc agggagacag ccccttgggc
       61 gcgcccggca cgacgcgata gccgcttgcg cgtagtgagc gagtccgcgg ccctcgggac
      121 gtctcgctcg cggccggaga gacaagggca tccgagagcg tgcgccggcc gcactgcggc
      181 gctgctggtc tgcgcgcacg gcgtcgccgg cggccccggc atggcgagcc ggcacgccca
      241 ggcgatcgcc ggccgtgcct tgttcgccgg ggtcgcggcc tgctgcctcg ccggccggcc
      301 gagcgtcgcc gaggccttgg ccgcgctcgc cggcgagccg gtctacctgg tgccggcgct
      361 gatggcggac ggctataccg cgcgcacgct gctcaagcgc gcgctcactg agacggcacc
      421 cgcggaccgc gcgcgcgtgg tggtgcggcc gccggccggc acccatgccg ggctcgcgcg
      481 gctgtgcctc ggcgccgcgc tgcgcgcctg cgcggtcgaa ggctggcacg ccgatgcggc
      541 gcggctggtg ctcgccgcgc acggcacgaa gcgcagcgcg cgctcagccg aggccgcgcg
      601 ccgccacgcc gaggcgatcg cgcacggcgc ccgtttcgcc catgtctcgt gcgggttcct
      661 cgagcaggag ccgaggctcg acgccgtgct cgccgagctg cccgcggtgc cgaccgtcgt
      721 ggtcgggctg ttcgccgacc gcggcggcca cggcgagggc gacgtcggcg ccctggtcgc
      781 ggcggcgccg gggccggcgc actacgccgg cccgatcggc accgaccccg gcttcgccga
      841 gctgatcgtc gcgcgtgcgc tggccgccga cgcctgagcg ggttgcaggc cgggcgtcag
      901 cggcgccgcg gtggcgcagc accggcggca cgtgctcatc tgctcgggcc gcgcgctcaa
      961 gtcgaggatc atgcccatcg cggcgagcgt ctggccgggc ccgcaccagt gcccagacca
     1021 gagtttggag tcgtccgcca ctcgacattg gggtaccccc acgcgcccgg caccgtcact
     1081 gatacgcgat gcaggtctcg cagggggtgc cggcgacgtc cttttggagg ttcgccgcgc
     1141 gcaaggcgcg gaacggcgcg ccctgccacg cttccaggaa agacatccgg ttgaggtcgc
     1201 ccatgtcgaa gcgcccgtcg tggtcgaagc agcacgccga cagccggccg tcataggtga
     1261 tgtggccttc ggtgaacagc gcccagcacg gcaacgactc gcgcagcgct cccacccggc
     1321 cgacgttgcc cgcgaccggc cgcgtaccgc gggcgcccgc ggtcagcccc gcctggttgt
     1381 agagcggcag ccagtaatgc tcgtcgacga acgggcggat ggtctcgacc gccgccgcca
     1441 tgcgcgcgcg ctggcctccg tcgtactgga tcgaggatgc gtagaggccg cagcggtggc
     1501 cggtctcggc ctcgaccttg tcgcgcacgc gccgcgcggc ggcgatgttc gcgaccactg
     1561 cgtcgaaggc atcgacctgg gtcacctcgc ggcattgcgc cgggtcggcc cagttgaacg
     1621 agaacttgag gctgtcgagg cccgcctcga tgcaggcgcg cacccggtcg ggcctggcca
     1681 ggcggccgtt ggtggtgagg aagatgtagg gatagccgag cgcgctggcg tgggcgatgg
     1741 catcgggcag gcgccggtac aggaacgact cgccgagata gaacatgccg atctcatcga
     1801 ccccagcctg ccgcatctcg gccagcaggc ggcgcagcag gtcccaatcg atgtcgccct
     1861 tggtgcgcag gccgtcgccg gtggcgcaga agaagcactt gaagtcgcag cgggcggtga
     1921 gctcgatctt gacgctcttc ggacacggcg gcgccgccgt gcggtattcg ggcgggataa
     1981 gggtgatcgc gtcgatgcgc tcagtgatca tggcaacctt ctctgctggc gcactcgaac
     2041 ctcgcacgag cggaagattt gccggccgtg tgcacggcct cgtttccatt ggagttagtg
     2101 ttgccgacgg gcagggcaat gcagccctcg cgcggtcgac ggcgcgggca gcggcggacc
     2161 ggcccgcgcc gtcacccgcg ccgctcgttg accgagcggc tacatgcctt gattgagcat
     2221 ttccccggtc ttggtcgcgg ccatctgcgc ctgcgccagg tcgtcgctcg cgaggccgtc
     2281 catggccacc gccaaggccg cctcgagctt ctgcttgatg gcggcatcgc cggcgtcggg
     2341 aatcgcgccg ctgccctggt cctggcacgg attggcctgg gtcgagtcga agccgtcgcc
     2401 gtccgggccg accaggcagt tgacggcatg atgaagatgg gcgcgcacca tctccaggtc
     2461 cgccgagtcg gcggcaagct gggcgtgctg ggcggcggtc ttggactcca tcatggcgtc
     2521 gccggcgagc gccccgggcg cgccggcgag agcggacagt gcgagcattg cggcagccgc
     2581 cgccgatgtc gaaagcctgt ggatcgtcgt catgaccttg cttcctctgc tgtgaagatc
     2641 ggtggtgttc gggaaccacg cgtgatcaac gcttgtaccc ggcgccccgt ggccggccag
     2701 ccgggcgtag cgaatgtggg cacgcgaccg caggcgccgc aagacgtcgc cgcatgaata
     2761 gcgccggcag cggcgggcca cccggccgcg atgtcgcgcg ccgagccttg agattaagtg
     2821 cgaatgcgtc gcagtcctat tgtaattgcg gcagccgcgt gctacctcag cgcagttgcg
     2881 agtaattctc aatcggagcc ggccagatga tccgatcgtc cttgcgccgc gggctcgcgg
     2941 ctttggtgct gggtgcggct ctcgccgcac cgtcggcggt ggccgacgag ccgagcttcg
     3001 agctcgtcat ccgcgaccac aagttcgagc ccgagacgct cgaggtgccg gccaacgccc
     3061 ggatcaagct gatcgtccag aacgccgatc cgacgcccga ggagttcgaa agctacgagc
     3121 tcaaccgcga gaaagtgatc cctggcaaca gccaggcgat cgtgtatatc ggccctctcg
     3181 atcccggcac ctatccgttc ttcggcgagt tccacatgga caccgcccag ggccggatca
     3241 ttgccgaata gtcccgcaac gacgaggcaa ggatcacgac catgtcggcg accgcactga
     3301 tcgtattccg cgaggtcctt gaggcggcgc tggtcgtcgg catcgtcatg gcggccacgc
     3361 gcgggctgcc cggtcgcaac gcgtggatcg gcgcaggcct tgcgctcggc gcgggcggcg
     3421 cggtgctggt ggcggccttc gcgggcgcga tcgccgcggc cgcgtcgggc atgggccagg
     3481 agctgttcaa tgccgcggtc ctgttcatcg cggtcgcgat gcttggctgg cacaacgtct
     3541 ggatgagccg ggcgggccgc gccatggcgc gcgagctgac cgccgtcggg cgggccgtgc
     3601 gcgacgccga gcggccgatc tacattctcg ccatcgtggt cggcgttgcc gtcctgcgcg
     3661 aaggctcgga ggtggtgctg ttcctctatg gcatcgccgc cgcccagggg accgacgccg
     3721 gcgccatgct cgccggcggg gcggtcggcc tcgcgctcgg catcgcggcg ggggcggtga
     3781 tctatttcgg gctgatccgg ctcgccggcc gctacctgtt cgcgatcacc ggcgggatga
     3841 tcctgctcct cgccgccggg atggcggcgc agggcgccaa gttcctgtcc caggccggct
     3901 atctgcccac cctcggccac ggcatctggg acacctcggc ggtgatctcc gacggcggcc
     3961 cgctcggcaa tctgttgcac gtcctgatcg gctactcggc ccgcccggac ggcatccagc
     4021 tcgtgttcta tatcgcgacg ctggttgcga tcggcggtct catgtacctg ttccgcacgc
     4081 ccaaggcgcg cgtggcggcg gtggcggcgg tggcgatcgt ggctgcggcg gcgacgttcg
     4141 ggccgaccgg cggcgagccg gcccgggccg ccgaccttaa ggtctattcg ccggtggtcg
     4201 aggaagggga gttcgcgatc gaggcgcgcg gcaacctcac cttcgacgac gccgcggaca
     4261 aggaccgggc ccagtcgcag cgctacgaga tcgagtacac gccgaacgac ttctggcaca
     4321 cggcgctggt cggcaagctg gaacaggcgg ccgagggcag cctgagatac accgcgaccg
     4381 catgggagaa catcctccag ctgttccccc agggccagca gtggctcgat ctcggcgtct
     4441 acctcgagta cgagctcgcc cgcgaccgca gcgacgccga cgcgttcgag ggcaagatcc
     4501 tggcggagaa gaacctcggc cgtgtcgcct tcacggtcaa cccgaccttc gagcaggaga
     4561 tcggcggcga cgacacggac tcgaccgagt tcaaatatgc ggcgcgcgtg aaatggcgct
     4621 ggaagccgga actagagccg gcgatcgagg cctatggcga catcggcgag atcggcgacg
     4681 taaaggcgat gcgcgcacag cgccatcagg tcggcccggt gctgctcggc acgttccgcc
     4741 tcaccgatac cagcaagctg cgctacgagg ccggctatct attcggactc acctccgatg
     4801 gcagccccga cggcgctttc aagtggctgg tggaggtcga gcactacttc tgatcggccg
     4861 cccccggcgg ctgcgcgcgc gccagcagcg gtccgcagac cgccaggtaa agcgcaaacg
     4921 cgaccatcca cgcggttgcc gacgtgatca caaggggcat gtaggcgtcg gtccaggcgg
     4981 aggcgatcag gcgcgtcagc gcggcgacgg tgaccagcac gtaggccgcg ctcaggcccg
     5041 gcccggcgcg gagttcgcgg ccggtatggc cgagcgtcgc ccggctcatc accgccatgg
     5101 tcatggttcc catcgcgccc gcggtcagcg cgtgaatggc gctggtctgg gacagtccct
     5161 cgaccacgaa gctcgccgcg agcagcgcca agccgatcgg gatccacaga tagccgacat
     5221 gcagcaccca cagcagcggc tcggctcggg tgcgccagcc gcgccagcgc gcaaggcgca
     5281 ccgcgttcag cgccgccgcc agccccgcga gcgcggccgc ggtcgcggta accggcgtcg
     5341 ccgcccacca cgccaaggcc agcgccgtcg cggccaggct cacgcggtcg acgacgccga
     5401 agctcgccgg caacgcctgc ggatcgcgct tgaccagcca gttgcggctg aagctcggga
     5461 tgatccgccc gccgatcagc gtgatcaggg cgatgatcac ggcgatcgcc gcgcgccggg
     5521 cgagcccgtc gtcgaccaag cccgcgaccg cgccgtgcga cagcaagttg caggcgagaa
     5581 acaggagcac ggccgacacc accggcaggt tgcgccagtt tttgccggcc acgacctcgc
     5641 gcagggccac cgcggcgacc gccgcgagga aggcgaggtc gacgaccgcg gccggccaca
     5701 taccgaccag cgccgaggcc gcgaccgcga tccgcccggc gagccacagc ccggccagcg
     5761 ccagcagcgg cccgccctgc agcggcaggc ggccggtcca gttggggatc gcggtgagca
     5821 gaaacccggt gacggtggcg gcgacgaagc cgaacagcat ctcgtgatag tgccaggcga
     5881 tcgggtcgaa ggcggtcggc agcgttatcc aaccccggat cgtcgccacc gacagcgcca
     5941 gcgccaccgg cgcccagatg ccggcgagaa ggaagaacgg gcggaagccg tgggcaagga
     6001 ccgccggcac ggcagcctgg cggtagcgcg gcagcggcgc gtggccggca accctgcccg
     6061 gttcgcgccc gctcggctgg tccgctcccg tggccatccc gcagcctcac taagatgccg
     6121 ccccgggctg cgagcattgg catgctccat ttcggctgcg ccggggaccc gcagcatctt
     6181 acggccttgc gccgcgaacg ggggaggatg tcgatcgagg cgccgccacg gaggtcgcga
     6241 ttgccggtcg cgcaacccgc ccgcgcctgc ctcacgccga ccgtcaatcc gtcactggcc
     6301 ggcggaggcg gttccggacg gcgacaggtc ggtcccgaac accggcccga cggcgcccga
     6361 gatcccggcc agaaccccga ggtcgagcag cccggcacgg tacatgtcgt ggaacacctg
     6421 gccccagccg ctcgaggtga gccgcgcgac cgcgatttgc tccaggctcc agatgtcgcc
     6481 ggtggcgggg cacgggccgt agccgtgcgc gatgtccacc gcgccggggc cgccagcatc
     6541 gcggtcgccg ccttgcatgg tctgcgccag gaacgcgatc tgcgccaggt acaggtgaag
     6601 cgcgacgtcc cggtggccgg ccggaagcga cgcaaaggtg cgcggccagg gttcgctgca
     6661 cacctcaggc cgtgaccggt ccagcggcac cgcgccgctg tccagggcgc gctgctcggg
     6721 ctcggcctcc gccgggcccg cagggaccgt cgccgcgacc tgcgcgggcg tgccgctgcc
     6781 gtcggctgcc gcgggagcgg ccgtcaccag cgatccgacc agcaggcaca gcatggcgca
     6841 ggcgccgttc gatcgcgggc gagtcccggt catgcgagcc ctccatttcg ccgtcccgca
     6901 ccgcacggtc ctgcgggcca tggccaaatg tttcgggaaa acgctaataa gtataatccc
     6961 tagtgcgtgc ggcgggggtg acaagcggcg catattgttg tgacaatttc cggatcgggc
     7021 tgggcaaccc cgtccgaccc cggatatcgt tatcctgcaa cggttttccg gctcaagcgg
     7081 ccgacggccg ggcgtcacaa aagattccgt gctataaaat cggcaggggt gcagaaatct
     7141 tgagcggggt cggcggcagt gtccattgcg ctggggtcag ccatccggtc cctgatcggg
     7201 accatcgtcc tgatgctggg cgggtgcgcc atggcctcgg cgacgcgcga ggacgtcgac
     7261 cgggtgtcgg cggacgagga ccacgccccg accgcgggcg gcgccaggcc gatcgtggcc
     7321 gtgcccgaga ccgagccgcc ggcgtcggtg ctgccggacg aggcggccgg cgagcagccg
     7381 gcggcgatcg agctttcggt gcgcacgccg ccggccaagc cgacgccgcc gcccaagccg
     7441 ctgccgtccc ccgacccgga caagctcttg ggcatgagca cgggcgcggt cacggcggag
     7501 ctcggcgcgc cggcggccca gcgcgacgag ccgccggcac atgtgtggcg ctacacgacc
     7561 gatttctgct cgctcgacgt cttcttcttc ctggacatga ccagcgacag ccgtcgcgtg
     7621 ctggcctacg acatccagat catcgacggg cgcagcgagg acgagctgct ctgctatcgt
     7681 gagatcgaga agagacatgc tgtcgcaagc gaaaacatcg acctcagata acaaacgaat
     7741 cctggtcgtc gacgacgacg acctgttccg cgagtcgatc gcggcgaacc tttcggaatc
     7801 cggtttcgac gtttcggcgt tcgccgacgg cgagagcttt ctcgacttca tgggcgaggg
     7861 gtgcggcgac ctcgttctgc tcgactggaa gatgccgggc atgtccggca tccaggtgct
     7921 gcaccgcatc cgcgagctcg ggatcgagct tccggtcatc ttcctcaccc atctcagcga
     7981 ccaggtctac gaggagcggg cgctcggcgg cggcgcggtg gatttcgtcg agaagacgcg
     8041 cagcttcacc atcatccagc gccgcatcga gctgatcctg agcgggcggc gggccggcgc
     8101 cgcgggaatc gccgacgcgg cctcggacca gaaaggcgtc ggcgtcatcc gttcggcgtc
     8161 gctcaccctc gatgccggct cgtgccgcgc gttctggaac gatctggagg tcgacctgac
     8221 actgaccgag ttcgagatcg tgcagtggct ggtcgagcgc gccgggcgcg acgtgcccta
     8281 ccgcgacctg tacgatctgg ttcacgggcc cgatttcatc gccggcctgg gatcggaggg
     8341 ctatcgcgcg aacgtgcgcg cgttcatcaa gcgcatccgc cagaagttcc ggaacctgga
     8401 cgacaacttc gatggcatcg tcaactatcc cggcttcggc taccgatgga gagcggatgg
     8461 cgactgaggc cggcgcgggc cggtcgggct tctcgctgct gcggcgttcg atcagcgtga
     8521 agttcctggt gctgttcctg ctgttctcga tcgtgccgtt catcatctac gaccagcttc
     8581 aacaggccta tctgcagcac cgcaccttgc tgctcaacag catccaggag caggggcgcc
     8641 tggtcgcgat cgggatcgcg ccgtggctgc cgtccgagac gccgaaggag ctgcgcgcga
     8701 ccggcaagct gctcgagcgc tacgattcgc gtcaggtcag catcaagctc ttgtggcggc
     8761 ccgccgcgac cctcgaggac tcgacgctct attacatcgg ctcggcgccg gcggtgcccg
     8821 ccgcctacct ggacgaggag gtgcggcgat ttcgcgagat gggcgtgatc tcgaacgtgc
     8881 tgggctcgtg cagcggcggc gggccggtcg cctcgcgcta cgtcaatccg gccggcgggc
     8941 acgagatcct caccgcgatc acgccgatcc tcaagggcga ggaatgctgg atcgtcatca
     9001 cctcggcatc ggaagaggtg cttggcgagg acgggctcgg caccccttac tggcaacgcc
     9061 gcgaggtggt gctgtccggc gtcatctatc tggtcgttct tgccgtcacg ctgtcgctgc
     9121 tgctgtcggt gtggggcaac gtccggcgct tcggctggct ggcgcggcgg ctgcggacgg
     9181 cgcgcgaacg gagccggcgc ttcgtcgacc tcaatcgcgt gcccgagctc gccggcgtgg
     9241 cgcaggcgct cgacgacctg gtgtcgtcgc tccgccgctc ggccgagagc ctgcgcgaga
     9301 gctcggagga acgggcgcac gccttcaaga cgccgatcgc caccatcgcg cagtcggtcc
     9361 agccgatccg caacacgctc ggcgacagcg acggcagggc ggggcgcgcg ctcaaggtca
     9421 tcgagcgctc cctggaaagg ctcgaggagc tcctggtcgc cagccaccgc atggacgagg
     9481 aaaatgccaa gctgatcgat ccgccgcgcg agcccgtcga cctggcggcc ctggtgcgcc
     9541 agaccgcaag cggctacgac cagagcctga aggcgcagga cagcgccatc gagatccgcg
     9601 cctcggtgcc cgacaaggcg gtcatcgtcg aggccagcga ggacctgatc gagaccgtgc
     9661 tcgagaacct gctcgacaac gccatcagct acgcgcccgc gaacggctgc gtcgaggtgg
     9721 cggtgaccgc gtccggtggc gccgccaggg tgaccgtcgc cgacaacggc ccgggcatgg
     9781 acccggccat gctgaaccgc gccttcgagc gctacttctc gacccgtgcc ggggggcggc
     9841 ggggcgacga cggcggcgag gcgggcgcac cgtcgcattt cggtatcggg ctatgggttg
     9901 tacggcgcaa catcgaggcg ctcggcggca gggtggcgct cgagaaccgc aacggcgcag
     9961 gcctggtggt gcgcgtcgat atcccgctcg cacgctagaa cggatcggcg gccgacgctt
    10021 gtcacagatt tgtcgctgcc ccgccggccg aggtcccgct acggtccgag ggccggcgca
    10081 acgcgccacg caacgcggag acgggatcgg ccgacgcgca aggcactcct tcggcactgc
    10141 caccgcaatt gcgacatggt cgtgggggat gcgccggcac cttctgcgcc cggcttcgtt
    10201 gcacgccccc cgaacagcgg cggccaacgc gcatcgcgcc cgcaagcgtc gccgaaaaaa
    10261 agaccccgca ggcaatgcgg ggtcgaagtc gctcacgtgg gggacgtcga cgtgagcatt
    10321 tcaagggggg gtctcttgtt gttgttgtgg gcacgtcacg tccgttgcgg gccgccgatc
    10381 ctggggtcgt ccgtgccggc cggcgccccg atcggggctg tgcccgcggt cgatggcctg
    10441 ggcacgggcc gcggccggaa gcagcgccgc gagcgcgatc agccatgttc tcatctcgat
    10501 gcctcggcgc aattgacgtc cccgcgttgc gcgtcggttg ccctgttgcc cttttctaac
    10561 cgaacgcggc gcgatgcggt tgtgaccggc gtgggacaaa tttgagtcgt ggtcccgtct
    10621 cgccttgcgg ccggcgtcgc ggtcgagtcg gtgccgggcg tgacatgcat cgacgccccc
    10681 cttgcggtgc tcccggccgc catcggcggc ctcggccggg cctgacgcgc gtgttcagcc
    10741 gacccgcgag ctccgcgccg gcagccggca gaacccctgc gccggcacat cgagcgcgct
    10801 gttgaacttg ctcgacaggt tctggaaggc gatcagggcg gtgagctcga tgatcgtatc
    10861 gtcgtcgaaa tggtgccgca gctccgcgaa cagcgcctcg tcgacctgct ggtcggtgcg
    10921 ggtgatcgcc tcggcgtacg cgagcgccgc ccgttcgcgc gcctcgaact gctcgctcgc
    10981 gcgccagtcg tcgagcgcct cgatcttggc cggcgcgacg ccgcgcttga gcagcgtcgc
    11041 cgagttgagg tcgacgcaga accggcacca gttgatctgc gacacccgca ccgtgaccag
    11101 cgagcgcagc gccggatcga gcggcgacga tttgcggtcg atcatgccgt agagcacggc
    11161 gacgcccagg aacagcttgg gcgcccgcgc ccacagcagc gccgagtcga gcaccgcgcc
    11221 gtatttccgc cgctggttcc agaagaacgg gcgcaggtac caggggtagc tcgccagcgg
    11281 tttcgcgtcg attctcatga cttgaccttt ccgggccggc gcggaccctt gatgacaaga
    11341 acgggccgaa ctgtcgctat catggatcag ggccggcgcg ccggcacgag cggccttaac
    11401 ctcggcggcc agacgtggga tcatgggccg atgcgtcaac gctgcgacac ggcaagggga
    11461 cggcccgggg cgtgccggcg atcctgaccc gggacgggct cgacgccctg ctcgccgcgc
    11521 tcaggggccg cggctaccgg gtggtcggcc cgaccgtgcg cgagtccgcg atcgtctacg
    11581 acgacatcgc cgccaccgcc gacctgcccg agggctggac cgacgagcag gacggcgggc
    11641 gctaccggct ggtgcggcgc ggcgacggcg cgctgttcgg ctacgcggtc gggccgcagt
    11701 cgtggaagaa gtacctgcac ccgccgatcc tgccgctgtg gcgggccagg cgcgacggcg
    11761 acgggctcag catcgaggcc accgagccgg tcagcgagcg cgtcgccttc attggcgtgc
    11821 gcgcctgcga gctgcacgcg atcgcgatcc aggaccgggt gctgctcgag ggccgctacg
    11881 tcgaccccca ctatcgggcg cggcgcgagg gcgcgttcat cgtcgccgtc aactgcggcc
    11941 acgccggcgg cacctgcttc tgcgtgtcca tgggcaccgg cccgcgcgcc gaatcgggct
    12001 gggatctggc gctgaccgag ctggcggggg agggcgagca ccggttcctg atcgaggcgg
    12061 gcaccgacgc gggccgcgag gtgctggccg cgctgcccca tcgcgccgcc cggcccgagg
    12121 aggtcgcggc cgccgaggcc gtgatcgcca agaccgcggc caacatgggg cgcgagatgc
    12181 cggcggccga ggtcaaggag ctgctgctcg ccaacctcga gcacccgcgc tgggaccagg
    12241 tcgccgatcg ctgcctcacc tgcggcaact gcaccatggt ctgcccgacc tgcttctgca
    12301 cctcggtcga ggattcgagc gatctcgacg gcgcgggcgc ggcgcgcacg cggcgctggg
    12361 actcgtgctt caccatggac ttctcgttca tccacggcgg cagcgtgcgc agctccgcgc
    12421 gctcgcgcta ccggcagtgg atgacccaca agctcgccac ctggcacgac cagttcggca
    12481 gctccggctg cgtcggctgc gggcgctgca tcacctggtg cccggtcggc atcgacatca
    12541 ccgaagaggt gcacgcgatc cgcggcggat ccgcgcgttg agggagggcg ccgtgcaagg
    12601 cctcgaacga atcgtcaggg agcacccgtt cttcgccggc ctcgaggagg gcttcctcga
    12661 gctcgtgtgc ggctgcgcca agaacgtccg cttcgacgcc ggcgactatc tgtgccacga
    12721 gggcgagcag gccaacgagt tctacatgat ccgccacggc cgcgtggggc tcgagatcac
    12781 cgtgccgggg cgtgcgtcgg tcaccttcca gaccatcggc gagggcggca tcgtcggcgt
    12841 gtcgtggctg gtgccgccct atcgttggaa ctacgacgcc aaggcgctcg agctggtgcg
    12901 cgcgatcgcg ctcgatgcga gctgcctgag ggagaagagc gagcaggacc acgacctcgg
    12961 ctacgagctg atgaagcgtt tcatgccgat cctggtgcag cggctgcagg cgacgcggct
    13021 gcagatcctc gacgtgtacg gaagcccgcg ctgacatgcg cgcgctcgcg cccgcgctcg
    13081 gtgatgcgat ggtgccgcgc atcgcgcgcg tgcgccggcg ccggcgcgag tgcccgcaga
    13141 cctggacgct cgagatcgag cccgagggag cgccgccgga tgtgcgcttc gcccccggcc
    13201 agttcaacat gctgtacgct ttcggggtcg gcgaggcacc ggtcagcgcc agcggcgacc
    13261 cggcggccgc gggcccgctg gtccacacca tccgcgcggt cggcgcggtc tcgactgcgc
    13321 tcgtccgcct cgggccggga gacgcggtcg gcctgcgcgg gccgttcggc agcggctggc
    13381 cgctggcgga agcggccggg cgcgacgtgg tggtgatcgc cggcgggctc ggccttgcgc
    13441 cgttgcgccc gctcatcctg cgcctgctcg ccgagcgcga gcgttacggc aaggtcgcgc
    13501 tgctctacgg cacccgcagc ccgctcgaca tcctttaccg gcgcgagctc gagacatggc
    13561 gccgccggct cgacagcgag atcgaggtca cggtcgacca cgccgccggc ggctggcggg
    13621 gcaatgtcgg cgtggtcacc gacctggtgc cgcgcgccgc cttcgacccg ctgcacacga
    13681 tcgccatggt ctgcgggccc gaggtcatga tgcggttcgc gatcgcggcg ctcatcgagg
    13741 cgggcgttgc cccggacgcg atctggctgt cgatggagcg caacatgaag tgcgccatcg
    13801 gccattgcgg ccattgccag ttcggcccgg cgttcgtgtg caaggacggc ccggtgttcc
    13861 gctacgaccg cgtccgtgac atcctggcgc tgaaggaaat ctgacatggc ccggggcgcg
    13921 cgcaagccaa ggctcgcggt gtggaagttc gcctcgtgcg acggctgcca gctcagcctg
    13981 ctcgattgcg aggacgagct gctggcggtc gccggcgcgc ttgacatcgc ctacttcccg
    14041 gaggcgatgc ggggcgaggt caagggccgc tacgacctgt cgctggtcga gggctcgatc
    14101 accaccgccg acgatgccga gcgcatcaag gaggtgcggc ggcgctcccg ccgcctggtc
    14161 accatcggcg cctgcgcgac ctccggcggc atccaggcgc tgcgcaactt caaggacgtg
    14221 gccgcgttca tctcgatcgt ctacgcgcgg cccgactaca tcgacagctt ggcgacttcg
    14281 accgcgatcg cggaccacgt gccggtcgat ttcgagctgc gcggctgccc gatcgacaag
    14341 cgccagctgc tcgaggtgat cggcgccttc ctggccgggc gcaagccggt cacgcccgcg
    14401 cacagcgtgt gcctcgagtg caagctgcgc ggcaccgtct gcgtcatggt cgcccacggc
    14461 acgccgtgcc tggggccggt gacccatgcc ggctgcggcg cgctgtgccc ggcctatgac
    14521 cgcggctgct atggctgctt cgggccgatg gagacgccga atgcgccgtc cttgagcggt
    14581 tggctcggcg ccctcggcat gggcgcgcgc gacctcgagc gtgtctggcg caccttcaac
    14641 gccaacgccc cggcgttccg cgaggaaagc gagcgccatg gcgcgtagga cgatccaggt
    14701 caagcagctc gcccgggtcg agggcgaggg cgcgctgaaa gtgacggtgc gcgacggcgt
    14761 ggtcagcagc gccgagctca ggatcttcga gccgccgcgc ttcttcgagg cgttcctgcg
    14821 cgggcgcgct tatcgcgagg cgcccgacat caccgcgcgc atctgcggca tctgcccggt
    14881 cgcctaccag atgagcgcgg tacacgcgat ggaggacgcg ttctccgtcg cggtcgatgc
    14941 gccgctccgc gatctcaggc gcctgctcta ttgcggcgag tggatcgaga gccacgtgct
    15001 ccacgtctac atgctgcacg cgcccgactt cctcggctat gacggcgcca tcgacatggc
    15061 gcgcgaccat cccgacctgg tccggcgcgg gctcgcgctc aagaaggccg gcaacgagct
    15121 gatggcgctg ctcggcgggc gcgagatcca cccgatcaac gtccgcgtcg gcggcttcta
    15181 caagaccccg cgccggcgcg agctggcggc ggtcggcgag cggctgaaat gggcgcgcga
    15241 cgcggcgctg gaaaccgtca cgtgggtcgc cggcctcgac gtcccggacc gggcttacga
    15301 ctacgagttc gtcgccctca ggcacgcccg cgactacccg ttcaacgacg gccgcatcgc
    15361 ctcgagccgc ggtctcgaca tcgccgccag cgagttcgac gaccacttcg aggagagcca
    15421 cgtcaagcac tcgaacgcgc tgcacgcgcg catccgcggc cgcggcgcct atctggtcgg
    15481 cccgctcgcc cgctacgcgc tgaacttcga gcgcctgtcg ccgctcgccc gcgaggcggc
    15541 gaaagacgcc ggcctcgggc cggtctgcac caacccctac cggagcatcg tcgtgcgcgc
    15601 ggtcgaggtg ctctatgcct gcgacgaagc gctgcgcctg atcgaggcgt accgcgagcc
    15661 ggaccgtccc gcggtgccgg tcgagccctg cgccggcacc ggccacgcct gcaccgaggc
    15721 gccgcgcggc atcctctatc accgctatcg gctggatgcc gacggcacca tccttgaggc
    15781 gcacatcgtg ccaccgacct cgcagaacca ggcgagcatc gaggacgacc tccgccgcta
    15841 cgtcgccggc tggctcgacc tcggcgacga cgagctgcgc catcgctgcg agcagacggt
    15901 gcgcaactac gacccgtgca tctcctgcgc gacccatttc ctcaagctgg agatggaccg
    15961 tggctgagcg ggcgcgccgc gtggtggtcg gcctcggcaa tcccgaccgc ggcgacgacg
    16021 gcgccgggcg cacggtcgcg cgcaggctgc gcgggcggct gccgcgggac gtcgccctcg
    16081 tcgagcacga cggcgaggcg agcggccttc tggcgcagat cgagggcgcc gatgccgcct
    16141 acctgatcga cgcctgtgcg tcgggggcgc cggccggcac cgtgcgccgg ttcgacgcgg
    16201 cccgcgcgcc gttgcccgag gcggccttcg aggtgtcgac ccacggcctc ggccttgccg
    16261 ccgcgatcga gcttgcgcgc gcgctcggcg cgctgccgcg ccggtgcgtc gtctatgcca
    16321 tcgagggcca gtcgttcgag gtcggggcag gcctgtcgcc tgcggccgcc gcggcggtcg
    16381 ccgaagtggt ggagcagctg gtgaaggaaa tccgtgcgcc ggagcgcgcc ggccatgcat
    16441 gaggcggggc tgatcgcggg cctgatgcgc cgcatcctcg aggtcgccga ggccgagaac
    16501 gcgacccgtg tggtcggcgc ctcggtgtgg ctcggcgcgc tcagccacat gtcggccgcg
    16561 cacttagccg agcacttcgc gcgggctgcc gccggcacca tcgccgaggg cgccgcactc
    16621 gacatcgagc tgtcgggcga tgcgacccac gccgaggctg acggcatcgt cctgcacagc
    16681 gtcgaggtcg agagctgacg cgatgtccgc gcccccgcct ttcgagctcg acatggcgga
    16741 gcgcgcgcgc ctgcgcatcc gggttcgcgg cgccgtgcag ggggtcggct tccgcccgtt
    16801 cgtctaccgg cgcgcggccg agctcggcct cgccggctgg gtgaccaact cgaccgaagg
    16861 ggtcacggtc gaggccgaag gcgatccggg tcgcctcgcc gcgctggtcg agtccttgcg
    16921 cgacgcggcg ccggcgcctg cgcgggtgtc gcaagtcgag acctgcgcga tcccgccatg
    16981 cgcggccgag gggttcgaga tccgggcgag cgtgaccacc ggcgcgcgca gcgccgaggt
    17041 gctgcccgac cttgccacct gcgaggactg cctggccgag ctgttcgacg catccgaccg
    17101 ccgctacctc tacccgttca tcaactgcac cagctgcggg ccgcgctaca gcatcatcga
    17161 ggacctgccc tacgaccgcg cgcgcacctc gatgcgccgg ttcgaaatgt gcgcggcctg
    17221 ccggtccgaa tacgacaacc cggccgaccg ccgcttccac gccgagccca acgcctgcgc
    17281 ggactgcggg ccgagcctcg cgctgtggga ctcgtcgggc gcgccggttg cgcacaagca
    17341 tgacgcgctg ctcgccgccg cgcgcatgct cggcgaaggg cgcatcgtcg cggtcaaggg
    17401 gctcggcggc ttccacctga tggtcgatgc acagagcgag gacgcggtgc gcgcgctgcg
    17461 ccggcgcaag cggcgccccg acaagccgtt cgcggtcatg ttcgcctccc tcgccgaggt
    17521 cgcgacgcac tgccgcgcct ctgcggccga ggccgcgctg ctcgtcggcc cggaacggcc
    17581 gatcgtgctg gtgcgccgca actccggcgc cgtcgcggat gcggtcgcgc cgggcaaccc
    17641 gtggctcggc gtcctgctcg cctatgcgcc gctgcatcac ctgctgcttg gcgcgctcgg
    17701 ctttcccgtg gttgcgacca gcggcaacct ctgcgacgag ccgatcgtca ccgacgagca
    17761 cgaggcggtg cgccgccttg ccggcatcgc cgacgcgttc ctggtccacg accgcgccat
    17821 cgtccgcccg ctcgacgact cggtcgtgcg cgtcgtcgcc gggcggatgc tcatgctgcg
    17881 ccgcgcgcgc ggctatgcgc cggcgccggt cggtgaagcc acactcgcgc ccggcatcct
    17941 cgctctcggc gggcacctca aggcgacggt tgccttgacc accgcaagcg gcgcaatcgt
    18001 gagccagcat atcggcgacc tcgagacggt cgaggcacgc gccgcctacc ggcgcgcggc
    18061 cgacgacatc gcgcgcctgc atgacgcccg gccgcgcctg gtggtgcgcg acctgcatcc
    18121 ggactacgac tcgagccgcg ttgccgaggc tgccggcctg ccggtcgtct ccgtcccgca
    18181 ccacctggcg catgtcgccg cgtgcctggc cgagcacggt ctcgatgcgc cggtgctcgg
    18241 tgtcgcctgg gacggcaccg gctacgggcc ggacgggaca gtgtggggcg gggagttcct
    18301 gcacgtcacc gcgcgcgcct ggcgccgggt cgcgcacctc gccaccttcc gcctgcccgg
    18361 cggcgaggcg gtggcacgcg agccgcgccg cgccgcgctc ggcgcgctct accgcgcgtt
    18421 cgggccggcc gcgctcgaca tgaccgacct cgcgccggtc gcggcattcg agccggccga
    18481 gcgccgcgtg ctgggcgcga tgctggcgcg cggcctgaac gcgccggtga cgtcgagcgc
    18541 gggtcggttg ttcgacgcgg tcgcggcgct ggccggggtg cgccagcgcg caagctatga
    18601 aggccaggcc gcggccgagc tcgaatgggc ggcgggcgag ggcgcaaacg gcgcgcgcta
    18661 cgacctcgcg cttcggcccg cggacggcga cgggccgctg gtggtcgact ggctgccggc
    18721 gctggcggcg atcgtcgcgg acctgcgcgc cggcatcccg gccgcgatcg tctcggcgaa
    18781 gttccacgac ggccttgccg ccgcgatcgc cgaggtcgcg gccgtgatcg gcgagccgcg
    18841 ggtggtgctc agcggcggct gcttccagaa caagcgcctc accgagtcga ccgtcgcggc
    18901 gctgcgggcg cggggtctcg agcccgtctg gcaccggcgc gtgccgccca acgacggcgg
    18961 catcgcgctc ggccaggcgg catgggcggc gtggtcagaa agcagaggag agacgccatg
    19021 tgcctggcgg tccccggacg aatcctgagc caatccggcg acgacccgct gatgcgggtc
    19081 gggcgggtcg acttcggcgg cgtggtcaag gagatcaacc tcgccttcgt gcccgaggca
    19141 cgtatcggcg accacgtact ggtccatgtc ggtttcgcca tcaccgtgat cgacgaggcc
    19201 gaggcggcgc gcgtgttcga gcatttgcgc gagatcggcg agctcgcaga ggacggggct
    19261 gcgccatgag gttccgcgac gagtatcgcg cgccggccgc cgcgcagggc tatgcgcgcg
    19321 cgatcgccaa ggcggcgacc cggccgtgga ccttgatgga ggtgtgcggc ggccagaccc
    19381 ataccatcgt ccgctacggc atcgaccagc tattgcccgt cggcatcgag ctggtgcacg
    19441 gcccgggctg cccggtctgc gtcaccccgg tcgaatatgt cgacaaggcg atcgagatcg
    19501 catcgcggcc gggggccacg ctgtgctcgt tcggcgacat gctgcgcgtg ccgggacgcg
    19561 gcggcgacct gctcggcgcc aagtcgcggg gcgccgacgt gcgcatcgtc tactcgccgc
    19621 ttgacgcgct cgccgtcgcc cgcgccgagc ccgcgcgcga ggtggtgttc ttcgcggtcg
    19681 gcttcgagac cacggcgccg gccaacgcca tggccgcctg gcaggctcgg cgccaaggcc
    19741 tcgccaactt ctcgctgctg gtctcgcacg tgctggtgcc gccggcgatg cgcgcgatcc
    19801 tggacgcgcc ggcgaaccgc atcgagggct tcctcgccgc cggccatgtg tgcacgatca
    19861 tgggcacccg cgagtacgag ccgatcgccg cccgctatcg cgtgccgatc gtggtcaccg
    19921 gcttcgagcc gctcgacatc ctcgaaggcg tctacatgtg cgtccgccag ctcgaggacg
    19981 gccgcgccgc ggtcgagaac caatatgtcc gctcggtccg gcgcgccggc aacctgcgcg
    20041 cgcaggcgct gatgggtgag gtgttccggg tgatcccgcg ccgctggcgc ggcatcggcg
    20101 acatcgccga cagcggcctc ggcctcgccg aggcttatgc cgatctcgac gccgagcgcc
    20161 gtttcggtgc cgtgcgaacg gttggcgagg ccgccggcga gtgcatcagc ggcctggtgc
    20221 tgcagggcgc gcgcaagccg cctgagtgcc cggccttcgg cacccgctgc acgcccgagc
    20281 ggccgctcgg cgtgaccatg gtctcgagcg agggcgcctg cgccgcctat taccgctatc
    20341 gcggcgcgcc gcgcccggcc gagaccgaga gcgccggcgc atgaccgcgg cgcgcggctt
    20401 cgcgcagagc tgcccgctgc cggccggcga cggcgagtgc gtgcagctcg cccacggcgg
    20461 cggcgggcgg gccatggcgc gcctgctcga tgcgctgatc cggccggcgt tcgacgaccc
    20521 cgagctcgac cgccgccacg acggcgcggc actggaggtt accggcccgg tggcgttcac
    20581 caccgacagc tacgtggtca agccgctggt cttcccgggc ggcgacatcg gctcgctcgc
    20641 ggtcaacggc acggtcaacg acctcgccat gtgcggcgcg cgcccgctct atctcagcgt
    20701 cgggctgatc gtggaggaag ggctcgcgct cgagaccttg cgcgccgtcg tcgcctcgat
    20761 cgccgccgcg gcgaaggcgg ccggggtgcg gctggtcacc ggcgacacca aggtggtcga
    20821 ccgcggcagc gccgacggcc tgttcgtcaa caccgccggc atcggcgaaa tcgtcgcgcc
    20881 ggcgccggtc ggcccggcgc aggtgcgcgc cggcgacgcg gtggtgctga gcggcgacat
    20941 cggccgccac ggcatcgcgg tcatggccgc gcgcgaggcg ctcggcttcg agaccgcgat
    21001 cgagagcgac tgcgcgccgc tcgccgcacc cgtgctggcg ctggtcgagt cagggatcgc
    21061 ggtccgctgc ctgcgcgacc tcacccgcgg cgggctggcg agcgcgctgg tcgagatcgc
    21121 cgagaccgcg ggcaagagga tcgcgatcga ggaggccgca atccccgttc gcgccgacgt
    21181 gtccgcggcg tgcgagctgc tcgggctcga cgcactccac gtcgccaacg agggccggtt
    21241 cgtcgccttc gtcgccgccg aggatgcggc gcgcgcagtc gaggtgctgc gccgttacga
    21301 cgcggccggc gccgccgccg tgatcggcgt tgtcggcgac ggcccgccgg gacaggtatc
    21361 gtgccgcggc gcgatcggcg ccagccgagt catcgacatg ctgagcggcg agcagctgcc
    21421 gagaatctgc taggccggcc gagcgggatg gcgagagcgg cgccgaaccg gaccgcgacc
    21481 ggcctcacgc caccgcctga gccgcgccga cgctggcacg cggagagcat cgagacggtg
    21541 ctcgccgccg tcgaggcgac gcctgaggga ctgacccagg cggcggtgga ggcgcgcctc
    21601 gcccggcacg gtcccaatcg cctgacgccg cccaggccgc gcggcgcgct cgccaggctt
    21661 ctcgcccagt tccacaacgt cctgatcgac gtgctgctgg tcgcggcggc ggtcaccgcc
    21721 ggcctcggcc actggaccga cagcgcggtc atcgccgcgg tggtcgtcgt c
//
LOCUS       DASWHU010000316        16945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3160792, whole genome shotgun sequence.
ACCESSION   DASWHU010000316 DASWHU010000000
VERSION     DASWHU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16945
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3160792"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VGB88_11720"
     CDS             complement(<1..470)
                     /locus_tag="VGB88_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9648160.1"
                     /translation="MFDIHRKEITWAGRPLILETGRMARQADGAVLVTYGDTTVLCTA
                     VAQRSPRPGIDFFPLTVNYQEKTFAAGKIPGGFFKREGRPTEKETLTSRLIDRPIRPL
                     FAKGFKNETQIIATVVSHDLENDPDIVAMVAVSAALTISGIPFMGPIGAARVGY"
     gene            complement(680..949)
                     /gene="rpsO"
                     /locus_tag="VGB88_11725"
     CDS             complement(680..949)
                     /gene="rpsO"
                     /locus_tag="VGB88_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9648161.1"
                     /translation="MSITVARKQEVIAGFATKAGDTGSPEVQVAILSERIARLTEHFK
                     DHAKDKHSRRGLLKLVSQRRSLLDYLKSRSPDRYQTLIARLGLRR"
     gene            complement(998..1969)
                     /gene="truB"
                     /locus_tag="VGB88_11730"
     CDS             complement(998..1969)
                     /gene="truB"
                     /locus_tag="VGB88_11730"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9648162.1"
                     /translation="MTADARTAPARLGAARRERRRRGRAVHGWVVIDKPAAMTSTRVV
                     ALVRRRLDAAKAGHGGTLDPLATGVLPIALGEATKTVGYVVDSTKGYRFTLRWGEGRD
                     TDDADGAVVETSPVRPTRAAIEAALPAFTGDIMQVPPDYSAVKVGGRRAYELARNDRS
                     VALAARPVRIDRLALVACADDQTASFEMDCGKGGYVRALARDLAHALGSCAHVVALCR
                     TRVGPFTLADAISLAELDRLADSGAAAQWLLPVARGLAGLPALSVNAEQAARLKRGQP
                     IYLVRPPHDAHGAPLAQGTVWATFADRPVAVAEFGGGELHPVRVFNF"
     gene            complement(1959..2360)
                     /gene="rbfA"
                     /locus_tag="VGB88_11735"
     CDS             complement(1959..2360)
                     /gene="rbfA"
                     /locus_tag="VGB88_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9648163.1"
                     /translation="MAAGARKGREPSQRQRRVGETLRHALAELLGRRELRDPALAERS
                     ITVSEVQVSPDLRTAVVYVSPLGGGDPEAVVEGLARAAPHLAAQLARMVKLKTMPRLS
                     FVADRSFDAAGRLGMILRDLESARPETDDDR"
     gene            complement(2556..5153)
                     /gene="infB"
                     /locus_tag="VGB88_11740"
     CDS             complement(2556..5153)
                     /gene="infB"
                     /locus_tag="VGB88_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9648164.1"
                     /translation="MTEKNESQNTKTLGLSRPGQLELKKTVEGGQVRQSFSHGRSKTV
                     QVEVKKKRTFRRGSSGRLTEIKKQVPTGAPGDAAALAEAEAHAAFRSLTGEERAARAR
                     ALESARLAEEDAQRQRVIEAHREAEEQRLRAEAEAFAAAVPAAAEAAPAEAAAPVPAA
                     AVAAPEAAAAEAKAAKPAPRRREETREEGEAAPKRRKGHGHRLSIERRDQKRRAGKMT
                     LVQALSDEERQRSLASIRRAREREKRAHARTGLEAHAKVVREVVVPEAITVQELASRM
                     AVRGAEVVKAMMKLGVMATINQVIDPDTAELVVGEFGHRIKRVSEADVEVGLSGPEDL
                     AEELHPRPPVVTVMGHVDHGKTSLLDALRKTDVAAGEAGGITQHIGAYQVEMPSGARI
                     TFLDTPGHAAFTQMRKRGANATDIVVLVIAADDGVMPQTVEAIQHAEAAGVPIIVAIN
                     KIDRPDANPEKVRNELLQHNIVVEEVGGDVLAVPVSATARTNLDRLEEAILLQAEVLE
                     LKANPDRPAEGVVIEAKRERGRGVVVTVLVQRGTLRVGDIMVAGSQWGRVRALVDDRG
                     RNIKAAGPSEPVEVLGLGDIPQAGEEMVVVSDEGRAREVSEYRKKLERDKAALAASRG
                     TVEQMLSQIAEGAVRELPIVVKADVHGSLEAILSNVAALTKEEKEVTARVLLAGVGGI
                     SESDITLAAASNAMVIGFNVRADAPARQLARRDGVDIRYYSVIYELLEDLRGMLSGLL
                     KPTERESLLGYARIKEVFSITKVGKIAGCEVTEGTVRRGAKVRILRDSVVIHDGTLSS
                     LKRHKDEVREVREGLECGMAFENYQDIREGDVIEVYDIEQVARELQPGAANLTMATYA
                     PSKDITSRK"
     gene            complement(5150..5806)
                     /locus_tag="VGB88_11745"
     CDS             complement(5150..5806)
                     /locus_tag="VGB88_11745"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9648165.1"
                     /translation="MANAISRTEAAPAPRARRLAPERRCAVTRQTRAQGELVRFVTAP
                     DGSLVVDLDGTLPGRGLWVGAERALIERALRKGVLARAGRVEADIADHIERGLARRCR
                     DLIGLARRAGQLVAGAEAVRAALAAGRAAVLIEARDGAPQARARLARLGRAVAPDLVV
                     VAVLDRAELGAALGRDDAVHLALAAGRLAGRLAIEAGRLAGFRPAAGDGDVAEETMRR
                     "
     gene            complement(5822..7393)
                     /gene="nusA"
                     /locus_tag="VGB88_11750"
     CDS             complement(5822..7393)
                     /gene="nusA"
                     /locus_tag="VGB88_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9648166.1"
                     /translation="MLNEVTPSLTRPELLQVADAVARDKSIDREIVIEAMEQAIQTAA
                     RRKYGQELDIVAHIDRASGEIRLSRRRAVVEEVEDEAIQIDLAQAQARDPALGAGDFL
                     IEPLPPIDLGRVAAQAAKQVIVQKVREAERERQHEEYKDRTGEIVNGLVKRVEYGNVI
                     VDLGRAEAIMRRDESLPRENFHNGDRLRAYIYDVRREPRGPQIFLSRSRPEFMAKLFA
                     QEVPEIYDGIIEIKAVARDPGSRAKIAVLSNDSSIDPVGACVGMRGSRVQAVVNELQG
                     EKIDIIQWNPDPATFIVNALAPAEVTKVVLDEDTRRIEVVVPDDQLSLAIGRRGQNVR
                     LASQLTGWEIDILTESQESERRTTEFRAITGLFMEALNVDDVIAHLLVTEGFTTVEAV
                     AFMPVEELAEIEGFDENLADELRERARDYLVRRDAELTEKRRALGVAEEVAEIEGLTP
                     AMLVALGEHDVKTLDDLADLASDELIDVVGEQQLDAESADAIIMAARAHWFADQPPPA
                     AGEGAAAEDRPQVEE"
     gene            complement(7400..7906)
                     /gene="rimP"
                     /locus_tag="VGB88_11755"
     CDS             complement(7400..7906)
                     /gene="rimP"
                     /locus_tag="VGB88_11755"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX9648167.1"
                     /translation="MSRATDDALETLIAPTASAMGYSLVRVRLTGSHPPRLQVMAERA
                     DGAGMTVDDCAGLSTAISALLDVEDPIAGRYELEVGSPGIDRPLVRLDDFKRFAGREA
                     KVETNAPVDGRKRFRGRLAGTDGDGIVLDLDAGRGRIVLPFAAVEEAKLVLTDELIRA
                     AMKTRAGD"
     gene            complement(8008..8769)
                     /gene="trmB"
                     /locus_tag="VGB88_11760"
     CDS             complement(8008..8769)
                     /gene="trmB"
                     /locus_tag="VGB88_11760"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX9648168.1"
                     /translation="MAGQRFLAEGDSPPGARRMVYGRRRGPALSPHRRQLLAELLPRI
                     AYPVHAAGRIARPLAPFPHRRWRGLWLEIGFGAGEHLAAQAAAHPDIAVIGCEPYVNG
                     VARLLSAIEAAALDNVRIWPDDARAVVEALPDRCLGRVFMLFPDPWPKTRHHKRRLLG
                     EPLLGELARVMRDGAELRLASDDRGYCRWMLARLVGHPAFAWMTRAAYDWQRRPADWP
                     QTRYEHKARAAGRGCVYLSFTRRRRRANSLKPAKN"
     gene            complement(8796..9965)
                     /gene="metK"
                     /locus_tag="VGB88_11765"
     CDS             complement(8796..9965)
                     /gene="metK"
                     /locus_tag="VGB88_11765"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9648169.1"
                     /translation="MPVRDYLFTSESVSEGHPDKVCDRISDAIVDAYMTTEDPNTRVA
                     CETLVTTNLIVIAGETRGPDISHARIEDIARAAVRDIGYEQPGFHWRHARIENYLHTQ
                     SPDIAQGVDSSGNKDVGAGDQGIMFGFACRETPVLMPAAIYFSHEILRSLARARHSGA
                     AAGLGPDAKSQVTLHYVAGKPVAAASVVVSTQHAADLDQEQVREIVRPHVVDVLPEGW
                     MCGEDDFYVNPTGRFVVGGPEGDTGVTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAARYLAKNVVAAGLADKCTIQLAYAIGVSKPLAVYVDTAATGEVDEERLAKVLQE
                     VMDLTPRGIRDRLGLVKPIYARTAAYGHFGREPQADGGFSWERTSLVEDLKSAFA"
     gene            complement(10080..10481)
                     /locus_tag="VGB88_11770"
     CDS             complement(10080..10481)
                     /locus_tag="VGB88_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9648170.1"
                     /translation="MTSKPNPIDIHVGGRIRLRRTLLGLSQEKLGKAIGLTFQQVQKY
                     ERGTNRVGSSRLYQIAKVLSVPVSFFFDDMAADPGAAAPQLAEDADPFKANPLANRET
                     LELVRAFQRIADPAVRKRIQDLVKAIAGSGE"
     gene            complement(10828..12399)
                     /gene="lnt"
                     /locus_tag="VGB88_11775"
     CDS             complement(10828..12399)
                     /gene="lnt"
                     /locus_tag="VGB88_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9648171.1"
                     /translation="MSRPPPTLLAGAARHLGALGGWRRALAAGLLGLCATLALPPLAL
                     VPLAVVAFSGLVWLAAGSATRTGWFALGFWFGLGHFLSGLYWVGNAFAPAGVSTWLAP
                     FAVVALAATCAVFPGLALLAARVSGATGVAGVVVLATAWTGAEWLRAFALFGGFPWNL
                     AGYVWMPSGAGIQLAALFGVFGLSWLAVLAAALPAQLAEARGAWRAVAAAWAAVALAH
                     AGGAVRLGIAEVAEVPGVELRLVQANISQFHKWQPALRERHLAKQLELSALPGVPPPS
                     HMIWPETATPYVLAEDGAVRAALAAVVPLGGALITGALRVETGDHGFRVWNSLEAVDG
                     AGRIVAAYDKARLVPFGEYVPLRSILDLAKLTDGETDFSRGPGPRTLAIPGLPPVSPL
                     ICYEAIFPGRVVDPQARPDWLLNVTNDAWYGDSAGPYQHFEQARLRAVEEGLPLVRVA
                     NTGISGVVDPYGRVTARLGLGEEGVIDAPLPRPAPLRTPYSRWRDWPLLALVVAVALW
                     SQRRRRQADARAKRS"
     gene            complement(12396..13331)
                     /locus_tag="VGB88_11780"
     CDS             complement(12396..13331)
                     /locus_tag="VGB88_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9648172.1"
                     /translation="MTENDLRPPRPSGRQRREADDRALVSVIGDWLRSLTGLGERRTS
                     LREAVERLIQQYRESGIAIDEQERVMLLNLLAFGGLRVEDVMVPRADIVAVPETATLA
                     EVIRVMSEAGHSRLPVYRDSLDDILGMIHVRDLIAFWHDHGAFSLAKAMRPLPFVPPS
                     MPVQTLLRQMRASKLHMALVVDEHGGIDGLVTIEDLVEEIVGEIEDEHDRAVAPAIVE
                     RADGSVVADARALIEDLEPVLGIDLLPADRDEEIDTVGGLVFDIAGRVPAAGEVISHP
                     AGLDIQILDADPRRVRRVRIVRGQAGQADAPAAHG"
     gene            complement(13328..13831)
                     /gene="ybeY"
                     /locus_tag="VGB88_11785"
     CDS             complement(13328..13831)
                     /gene="ybeY"
                     /locus_tag="VGB88_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9648173.1"
                     /translation="MSGPAGSGDEVAVAIADDGWLSAVPDAAALCARAARAALRQAGE
                     AGEVSIVLADDGRVAELNARYRGKEGATNVLAFPMADAGAAAAGAPRLLGDVVLARET
                     VVREARAQAKDVADHMAHLVAHGVLHLVGHDHHASAAAEAMEALETAALASLGIADPY
                     RLPRDVA"
     gene            complement(13828..14865)
                     /locus_tag="VGB88_11790"
     CDS             complement(13828..14865)
                     /locus_tag="VGB88_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9648174.1"
                     /translation="MTRPAGATDAAKDATPLYLAFDDNALLGALYGEHDRNLARIEQK
                     LGVWVASRGNRVAITGRPDAAERAKRVLSELYDMLARGIPVGTAEVDGVLRMAAGRDA
                     GAPGPARLSAEDSVVTTQKRRIMARSPMQAAYVRAIYDHELVFGIGPAGTGKTYLAVA
                     VAASMLARGQVDRIILSRPAVEAGERLGFLPGDMREKVDPYLRPLYDALYDMMPAERV
                     VDRIQSGEIEIAPLAFMRGRTLANAFVILDEAQNTTPVQMKMCLTRLGENSRMVVTGD
                     PSQVDLPVGNRSGLKDALVVTADIDGVEVVRFTDADVVRHELVTRIVRAYDEFERNGR
                     PGAAEDRERPR"
     gene            complement(14862..16292)
                     /gene="miaB"
                     /locus_tag="VGB88_11795"
     CDS             complement(14862..16292)
                     /gene="miaB"
                     /locus_tag="VGB88_11795"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9648175.1"
                     /translation="MHHPDPGPAATAGRKAAARARAPAANKLYLKTYGCQMNAYDSAR
                     MAEVLAPLGYAPVARAEDADMVILNTCHIREKASEKVYSELGRLRPLKAEKAAAGGRM
                     ILAVAGCVAQAEGAEVIRRAPFVDIVLGPQTYHRLPEMVARAHRAAGAPVLDTSFPVE
                     AKFDHLPEARAAGGVAALVSVQEGCDRFCTFCVVPYTRGAEYSRPVAQVVAEVERLVA
                     AGAREITLLGQNVNAYHGEGPDGRAWGLARLIGRLAGIEGLRRLRYTTSHPRDMDPEL
                     IAAHRDVPALMPYLHLPVQSGSDRVLAAMNRGHDADRYRRIVAELRAARPDLALSSDF
                     IVGFPGESARDVDDTLALVEEIGFAAAFSFKYSARPGTPAAAAAGQVPEAEKRARLTA
                     LQALLADQQRAFNQATVARTLPVLLEKRGRHPGQLAGRSPYLQPVHLAAPAAAIGDVV
                     EVRITAVLANSLLGEVVAAPAAEGRA"
BASE COUNT         2274 a         6262 c         5996 g         2413 t
ORIGIN      
        1 atgtagccca cgcgcgcggc gccgatcggc cccatgaagg gaatgccgga aatggtcagc
       61 gcggcggaga ccgcgaccat ggcgacgatg tcgggatcgt tctcgaggtc gtggctgacg
      121 acggtggcga tgatctgggt ctcgttcttg aagcccttgg cgaacagcgg acggatcggc
      181 cggtcgatca ggcgcgaggt cagcgtctcc ttctcggtcg ggcgcccctc gcgcttgaag
      241 aagccgcccg ggatcttgcc cgcggcgaac gtcttctcct ggtagttgac ggtcagcggg
      301 aagaagtcga tacccggtct gggcgacctc tgggcgaccg ccgtgcacag caccgtggtg
      361 tcgccgtagg tcaccagcac ggcgccgtcg gcttggcgcg ccatgcgccc ggtctcgagg
      421 atcagcgggc gcccggccca ggtgatctcc ttgcgatgaa tgtcgaacat gcagcccttt
      481 cctcagcctg cctgccgcgg ccgccccatg cggccgcggg atggtccgcg ctgcgcccaa
      541 gcgccgcgga caaacgaaaa tgcccggccg cagcccgacc ggacacgccg accggcgccg
      601 tccgcgcgcc ggcacggaat cgcgccggcg gtgccggccg gtcccgtcgc gacgccgctg
      661 ccaccaccgg ggtcgggtcc taccggcgca gccccagacg cgcgatcagc gtctgatagc
      721 ggtcggggct gcgcgatttc aggtagtcga gcaggctgcg gcgctggctg accagcttca
      781 gcaggccgcg ccgcgaatgc ttgtccttgg cgtggtcctt gaagtgctcg gtcaggcgcg
      841 cgatgcgctc gctcaggatc gccacctgga cctcgggcga tccggtatcg ccggccttgg
      901 tcgcgaaccc ggcgatcacc tcctgcttgc gtgcgacggt tatcgacatg actcaaccaa
      961 ctccaaccgg cgaatccgcc cttctacgcc aggcgggcta gaaattgaag acccgaaccg
     1021 ggtgcagctc gccgccgccg aattcggcga ccgccaccgg ccggtcggcg aacgtcgccc
     1081 aaacggtgcc ctgggcgagc ggagcgccgt gggcgtcgtg cggcggacgg accaagtaga
     1141 tcggctgccc gcgcttcagt cgcgcggcct gttcggcatt gaccgacaag gccggcaggc
     1201 cggcaagccc ccgcgccacc ggcagcagcc actgtgcggc ggcgccacta tcggcaaggc
     1261 ggtcgagctc ggcaagcgaa atcgcgtccg caagcgtgaa cgggccgacc cgggtgcggc
     1321 acagcgcgac cacgtgggcg cagctgccga gcgcgtgggc gaggtcgcgg gcgagcgcgc
     1381 gcacatagcc gcccttgccg cagtccatct cgaagctcgc ggtctggtcg tcggcgcagg
     1441 cgaccagggc caggcggtcg atgcgcaccg ggcgcgccgc gagcgccacg ctccggtcgt
     1501 tgcgcgccag ctcataggcc cgccggccgc cgaccttgac cgccgaatag tccggcggca
     1561 cctgcatgat gtcgccggtg aaggcgggca gcgcggcctc gatcgcggcc ctggtcgggc
     1621 gcaccgggct ggtctcgacc accgcgccgt cggcgtcgtc ggtgtcgcgc ccctcgcccc
     1681 agcgcagcgt gaagcggtag cccttggtgc tgtcgaccac gtagccgacg gtcttggtcg
     1741 cctcgccgag cgcgatcggc agcacgccgg tggccagcgg gtcgagggtg ccgccgtggc
     1801 ccgccttggc ggcatcgagc cggcggcgga ccagcgcgac cacgcgcgtc gaggtcatcg
     1861 cggccggctt gtcgatcacc acccagccgt ggaccgcgcg cccgcgccgg cggcgctcgc
     1921 ggcgggcggc gccgagacgc gcgggtgcgg tcctggcgtc agcggtcatc gtcggtctcg
     1981 ggccgggcgg actcgaggtc gcgcaggatc atgccgagcc gcccggcggc gtcgaacgag
     2041 cggtcggcga cgaagctgag ccgcggcatg gtcttgagct tgaccatgcg ggcgagctgg
     2101 gcggcgagat gcggcgccgc ccgggcgagg ccctcgacca ccgcctcggg gtcgccgccg
     2161 ccgagcgggc tgacatagac caccgcggtg cgcaggtcgg ggctgacctg cacctccgag
     2221 accgtgatcg agcgctcggc aagcgccggg tcgcgcagct cgcgccgacc gagcagctcg
     2281 gccagcgcgt gcctgagcgt ctcgccgacg cgccgctggc gctggctcgg ctcgcgccct
     2341 ttcctggccc cggcagccat cgtcgtgaca ccaaaccccg cggaccgcta ggccgagtcc
     2401 cgcccgcgcc gacgtcgtcc cccctcaccc agctccggga aaggcgcggc atacgccgcg
     2461 ccaaccctgc gcaaccctct ccccccgcgc gcgggggaag agggaagggt gagggggccg
     2521 atcgaagcta agcgggccat tctctagatc gcaggtcact tgcgggacgt aatatcctta
     2581 gacggcgcat aggtggccat cgtcaaattt gccgcgccgg gctgcagctc gcgcgcgacc
     2641 tgctcgatgt cgtagacctc gatcacgtcg ccctcgcgga tgtcctggta gttctcgaac
     2701 gccatgccgc attcgagccc ttccctgacc tcgcgcacct cgtccttgtg gcgcttgagg
     2761 ctggacagcg tgccgtcgtg gatgaccacg ctgtcgcgca ggatgcgcac cttggcgccg
     2821 cggcgcacgg tgccctcggt gacctcgcag ccggcgatct tgccgacctt ggtgatcgag
     2881 aacacctcct tgatgcgcgc gtagcccaag aggctctcgc gctcggtcgg cttgagcagc
     2941 ccggacagca tgccgcgcag gtcctcgagc agctcgtaga tcaccgagta gtagcggatg
     3001 tcgacgccgt cgcgacgcgc gagctggcgc gccggggcgt cggcgcggac gttgaacccg
     3061 atcaccatcg cgttcgacgc cgcggcaagc gtgatgtcgg actcgctgat gccgccgaca
     3121 ccggccagca gcacgcgcgc ggtcacctcc ttctcctcct tggtcagggc ggcgacgttc
     3181 gacaggatcg cctccagcga gccgtggacg tcggccttga ccacgatcgg cagctcgcgc
     3241 accgcgccct cggcgatctg cgacagcatc tgctcgacgg tgccgcggct ggcggcgagc
     3301 gccgccttgt cgcgctcgag cttcttgcgg tactcgctga cctcgcgcgc ccggccctcg
     3361 tcggaaacca ccaccatctc ctcgcccgcc tgcgggatgt cgccgaggcc gagcacctcg
     3421 accggctccg acggcccggc cgccttgatg ttgcggccgc ggtcgtcgac cagggcgcgc
     3481 acccggcccc actggctgcc ggcgaccatg atgtcgccga cccggagcgt gccgcgttgg
     3541 accagcaccg tcaccaccac gccgcgcccg cgctcgcgct tggcctcgat caccacgccc
     3601 tcggcggggc ggtccgggtt ggccttgagc tcgagcacct cggcctgcag caggatcgcc
     3661 tcctcgagcc ggtcgagatt ggtcctggcg gtcgccgaga ccggcaccgc cagcacgtcg
     3721 ccgccgacct cctcgaccac gatgttgtgc tgcaacagtt cgttgcgcac cttttccggg
     3781 ttcgcgtcgg ggcggtcgat cttgttgatc gcgacgatga tcggcacccc ggccgcctcg
     3841 gcgtgctgga tcgcctcgac cgtctgcggc atcaccccgt cgtcggcggc gatcaccagc
     3901 accacgatgt cggtggcgtt ggcgccgcgc ttgcgcatct gggtgaacgc ggcgtggccg
     3961 ggcgtgtcca ggaaggtgat gcgcgcgccc gacggcatct cgacctgata ggcgccgatg
     4021 tgctgggtga tgccgccggc ctcgccggcg gcgacgtcgg tcttgcgcag cgcgtccaga
     4081 agcgaggtct tgccgtgatc gacatggccc atgaccgtga ccaccggcgg gcgcgggtgc
     4141 agctcctcgg ccaggtcctc cggcccgctc aggccgacct cgacgtcggc ctcgctgacc
     4201 cgcttgatcc ggtggccgaa ctcgccgacc accagctcgg cggtatccgg gtcgatgacc
     4261 tggttgatgg tcgccatgac gccgagcttc atcatcgcct tgaccacctc ggcgccgcgc
     4321 accgccatgc ggctggcaag ctcctggacc gtgatcgcct cgggcaccac cacctcgcgc
     4381 accaccttgg cgtgcgcctc gagcccggtc ctggcgtggg cgcgcttctc gcgctcgcgg
     4441 gcgcggcgga tcgaggccag gctgcgctgg cgctcctcgt cgctgagcgc ctggacgagg
     4501 gtcatcttgc cggcgcggcg tttctggtcg cggcgctcga tcgacagccg gtggccgtgg
     4561 cccttgcgcc gcttgggcgc ggcctcgcct tcctcgcgcg tctcctcgcg ccggcgcggg
     4621 gcgggcttgg cggccttcgc ctcggcggcg gcggcctccg gcgccgccac ggccgcggcc
     4681 ggcaccggcg cggcggcctc ggcgggcgcg gcctcggccg cggcgggcac ggccgcggcg
     4741 aacgcttccg cctcggcgcg cagcctctgt tcctcggcct cgcgatgggc ctcgatcacg
     4801 cgctggcgct gggcgtcctc ctcggccagg cgggcgctct ccagcgcgcg ggcgcgggcc
     4861 gcacgctcct cgccggtcag gctgcggaac gcggcatggg cctcagcctc ggcgagcgcc
     4921 gcggcgtcgc ccggcgcccc ggtcggcacc tgcttcttga tctcggtcag gcgcccggac
     4981 gagccgcgcc tgaaggtgcg cttcttcttg acctcgacct gcaccgtctt gctgcgcccg
     5041 tgcgagaagc tctggcgcac ctggccgccc tcgacggtct tcttgagctc gagctggccg
     5101 ggccgggaaa gaccaagcgt cttggtgttc tgggactcgt tcttctcggt catcgcctca
     5161 tggtctcctc agccacgtcg ccatcgcccg cagccggccg gaacccggcc aggcgcccgg
     5221 cctcgatcgc caggcgcccg gccagccggc cggccgcgag ggcgaggtga accgcatcgt
     5281 cgcggccgag cgcggcgccg agctcggcgc ggtcgagcac cgccaccacg accaggtcgg
     5341 gcgccaccgc gcggcccagt cgcgcgagcc gcgcccgcgc ctggggcgcg ccgtcgcgcg
     5401 cctcgatcag caccgcggcg cggccggcgg cgagcgcggc gcgcaccgcc tcggcgcccg
     5461 cgaccaactg gccggcgcgg cgggcaagcc cgatcaggtc gcggcagcgg cgggcgaggc
     5521 cacgctcgat atggtcggcg atgtcggcct cgacgcgccc ggcgcgggcg aggacgcctt
     5581 tcctcagtgc ccgctcgatc agcgcccgct cagcgccgac ccacaggccg cggccgggca
     5641 gggtgccgtc gaggtcgacg accaggctgc cgtcgggggc ggtgacgaag cgcaccagct
     5701 cgccctgcgc gcgcgtctgg cgggtcaccg cgcagcgccg ctccggcgcc agccggcgtg
     5761 cgcgcggcgc gggcgccgcc tcggtgcggg agatcgcgtt ggccatggtc accgcggctg
     5821 gtcactcctc gacctgcggc cggtcctcgg ccgcggcgcc ctcgcccgcg gccggcggcg
     5881 gctggtcggc gaaccaatgg gcacgcgccg ccatgatgat cgcgtcggcg ctctcggcat
     5941 cgagctgctg ctcgccgacg acgtcgatca gctcgtcgct ggcgaggtcg gcgaggtcgt
     6001 cgagcgtctt cacgtcgtgc tcgccgagcg ccaccagcat cgccggcgtc aggccttcga
     6061 tctcggccac ctcctcggcg acgccgagcg cgcgccgctt ctcggtcagc tcggcgtcgc
     6121 ggcgcaccag gtagtcgcgc gcgcgctcgc gcagctcgtc cgccaggttc tcgtcgaagc
     6181 cctcgatctc ggccagctcc tcgaccggca tgaaggcgac cgcctcgacc gtggtgaagc
     6241 cctcggtgac cagcaggtgg gcgatcacgt cgtcgacgtt gagcgcctcc atgaacagcc
     6301 cggtgatggc gcggaactcg gtggtccgcc gctcggactc ctggctctcg gtcaggatgt
     6361 cgatctccca gccggtgagc tgcgaggcaa ggcgcacgtt ctgcccgcgc cggccgatgg
     6421 cgaggctgag ctggtcgtcg gggaccacca cctcgatccg ccgggtgtcc tcgtcgagca
     6481 ccaccttggt cacctcggcc ggcgccaacg cgttgacgat gaaggttgcc gggtccgggt
     6541 tccactggat gatgtcgatc ttctcgccct gcagctcgtt gaccaccgcc tgcacgcggc
     6601 tgccgcgcat gccgacgcag gcgccgaccg ggtcgatgct cgaatcgttc gacagcacgg
     6661 cgatcttggc ccggctgccg ggatcgcgcg ccaccgcctt gatctcgatg atgccgtcgt
     6721 agatctcggg cacctcctgg gcgaacagct tggccatgaa ctcgggccgc gagcgcgaca
     6781 ggaagatctg cgggccgcgc ggctcgcggc ggacgtcgta gatgtaggcg cgcagccggt
     6841 cgccgttgtg gaagttctcg cgcggcagcg attcgtcgcg gcgcatgatc gcctcggcgc
     6901 ggccgaggtc gacgatcacg ttgccgtact cgacccgttt caccaggccg ttgacgatct
     6961 cgccggtgcg gtccttgtac tcctcgtgct ggcgctcgcg ctccgcctcg cgcaccttct
     7021 gcacgatcac ctgcttggcc gcctgggcgg cgacgcggcc gaggtcgatc ggcggcagcg
     7081 gctcgatcag gaagtcgccc gcgccgagcg cgggatcgcg cgcctgcgcc tgagcgaggt
     7141 cgatctggat cgcctcgtcc tcgacctcct cgaccaccgc gcgccggcgg ctcaagcgga
     7201 tctcgccgct ggcgcggtcg atgtgggcga cgatgtcgag ctcctggccg tacttgcgcc
     7261 gcgccgcggt ctggatcgcc tgctccatcg cctcgatcac gatctcgcgg tcgatcgact
     7321 tgtcgcgcgc caccgcgtcg gccacctgca gcagctcggg ccgggtcagg cttggagtca
     7381 cttcgttcaa catgatcacc taatcgccag ctctcgtttt catcgccgcc ctgatcagct
     7441 catcggtcag caccagcttg gcttcctcga ccgccgcgaa cggcagcacg attcgtcctc
     7501 gcccggcgtc gaggtcgagg acaatgccgt cgccgtcggt ccccgccagc cggccgcgaa
     7561 accgcttgcg cccgtccacc ggcgcgttgg tctcgacctt ggcctcgcgc cccgcgaagc
     7621 gcttgaagtc gtcgaggcga accagcggcc ggtcgatccc aggcgagccg acctcgagct
     7681 cgtagcgtcc ggcgatcggg tcctcgacgt cgagcagcgc agagatcgcc gtgctcagcc
     7741 cggcgcagtc gtccacggtc atgcccgcgc cgtcggcccg ttcggccatc acctgcagcc
     7801 gcggcgggtg cgatccggtc aggcggaccc gtaccagact atagcccatt gcggacgcgg
     7861 ttggcgcgat cagagtttcg agcgcgtcgt ccgtggctcg agacaccttt gcttcccggt
     7921 cgccgaaaac aaaaaagcgg gcacgcggcc cacggttcct aaagtgccct tcagcgcagg
     7981 cgtctttatt ctttcgctcc tatatactca atttttcgcc ggcttcaagc tgttcgcgcg
     8041 gcggcggcgg cgggtgaagc tgagatagac gcagccgcgc cccgccgcgc gcgccttgtg
     8101 ctcgtagcgg gtctgcggcc agtcggcggg ccggcgctgc cagtcgtagg ccgcgcgcgt
     8161 catccaggcg aacgccggat ggccgaccag ccgggcgagc atccaccggc agtagccgcg
     8221 atcgtcgctc gccagcctga gctcggcgcc gtcgcgcatc acccgcgcga gctcgccgag
     8281 caacggctcg ccgagcagcc ggcgcttgtg atggcgggtc ttcggccacg ggtccggaaa
     8341 cagcatgaac acccgcccga ggcagcgatc ggggagcgcc tcgaccacgg cgcgggcgtc
     8401 gtcgggccag atgcggacgt tgtcgagcgc cgccgcctcg atcgccgaca acagccgggc
     8461 gacgccgttg acataaggct cgcagccgat gaccgcgatg tcggggtggg cggccgcctg
     8521 cgccgccagg tgctcgccgg cgccgaagcc gatctcgagc cacaggcccc gccagcgccg
     8581 gtgcgggaac ggcgccagcg gccgtgcgat gcgcccggcg gcgtgcaccg gataggcgat
     8641 gcgcggcagc agctcggcca gcagctggcg ccggtgcggg ctgagcgccg ggccgcgccg
     8701 gcgaccgtag accatgcgcc gcgcgcccgg cggcgagtcg ccctcggcga ggaatcgttg
     8761 cccggccatg gacccgcgcc cgcgacgcgc gcgcctcagg cgaacgccga cttgaggtcc
     8821 tcgaccaggc tggtgcgctc ccacgagaag ccgccgtcgg cctgcggctc gcggccgaaa
     8881 tgaccatagg ccgcggtgcg cgcgtagatc ggcttgacca ggcccagacg gtcgcggatg
     8941 ccgcgcggcg tgagatccat cacctcctgc aggaccttcg ccaggcgctc ctcgtcgacc
     9001 tcgccggtgg cagcggtatc gacatagacc gcgagcggct tcgacacccc gatcgcgtag
     9061 gcgagctgga tggtgcattt gtcggccagg ccggcggcga ccacgttctt ggccaggtag
     9121 cgcgcggcat aggccgccga gcggtcgacc ttgctcgggt ccttgcccga gaacgcgccg
     9181 ccgccgtgcg gcgccgcgcc gccataggta tcgacgatga tcttgcgccc ggtcaccccg
     9241 gtgtcgcctt ccggcccgcc gaccacgaac cggccggtcg ggttgacgta gaaatcgtcc
     9301 tcgccgcaca tccagccctc gggcagcacg tcgaccacgt gcgggcggac gatctcgcgc
     9361 acctgctcct ggtcgaggtc ggcggcgtgc tgggtggaca ccaccaccga ggccgcggcg
     9421 accggcttgc cggcgacata gtggagcgtc acctggctct tcgcgtccgg gccgagcccc
     9481 gccgccgcgc ccgaatgccg ggcgcgggcc agcgagcgca ggatctcgtg gctgaagtag
     9541 atcgccgccg gcatcagcac cggcgtctcg cggcaggcga agccgaacat gattccctgg
     9601 tcgcccgcgc cgacatcctt gttgccgctc gaatcgacgc cttgggcgat gtcgggcgac
     9661 tgggtgtgga ggtagttctc gatgcgcgcg tggcgccagt ggaagccggg ctgctcgtag
     9721 ccgatgtcgc gcaccgccgc gcgcgcgatg tcctcgatcc gggcgtggct gatgtcggga
     9781 ccgcgcgtct cgccggcgat cacgatcagg ttggtggtca ccagggtctc gcaggcgacc
     9841 cgggtgttcg ggtcctcggt ggtcatgtag gcgtcgacga tggcatccga gatgcggtcg
     9901 cagaccttgt cggggtgccc ctcggacacc gactcgctgg tgaacaggta atcgcgcaca
     9961 ggcatgtgcc ctccccggcg ccgctgcgat ggtcgggaac ccacagccgc gccccggcgg
    10021 ccgcgcgcag tcttatatca aaggcgcgcc ggcgcgaccc gcttgatgcg cgggcagggt
    10081 cactcgccgg agccggcgat cgccttgacc aggtcctgga tgcgcttgcg caccgccggg
    10141 tcggcgatgc gctggaacgc ccgcaccagc tcgagcgtct cgcggttggc gagcgggttg
    10201 gccttgaacg ggtcggcgtc ctcggcgagc tgcggcgcgg cggcgcccgg gtccgccgcc
    10261 atgtcgtcga agaagaacga caccggcacg ctgagcacct tggcgatctg gtacaggcgg
    10321 ctggagccga cgcggttggt accgcgctcg tatttctgga cctgctggaa ggtgagcccg
    10381 atcgccttgc cgagcttctc ctggctcaag ccgagcaggg tgcggcgcag gcgaatcctg
    10441 ccgccgacat ggatatcgat ggggttgggc ttggaagtca tcccggttca ctccgttgcg
    10501 accagtcgtt cgccccgccg gcacgaacag ccttgtctca agatcgacgt cacggcgcgc
    10561 atccgtgacc agccgcgcgc cctcgcgcgc ccggcgagct ggcgggcccc ccggggccac
    10621 gcgcgacgcc tggcgtcacc cgaggccgtg ccgaactcgg cccgcgtccg ccgcccgtcc
    10681 ccgtcagtag cgggcacgat ggccgagtgg ctggcaatat ccccaaccgt gccgcagttg
    10741 tctacgaaat tgcagcccct atggcgcaca aaccctggaa attttcttcc ccgacagtcg
    10801 ccggacaccg ttccgccagc gaaccgttca gctccgcttc gcccgggcgt ccgcttgccg
    10861 gcggcggcgt tgcgaccaca gcgcgaccgc caccacaagc gcgagcagcg gccagtcgcg
    10921 ccaccggctg tagggggtgc gcaacggcgc cggccggggc aggggggcat cgatcacccc
    10981 ctcctcgccg aggccgaggc gcgccgtcac ccgaccgtac gggtcgacca cgcccgagat
    11041 gccggtgttc gccacccgca ccagcggcag cccttcctcg accgcgcgca gccgcgcctg
    11101 ctcgaaatgc tggtaggggc cggcgctgtc gccgtaccag gcgtcgttgg tgacgttgag
    11161 cagccagtcg gggcgcgcct gcggatcgac cacccggccg gggaagatcg cctcgtagca
    11221 gatcagcggg ctcaccggcg gcaggccggg gatggccagc gtgcgcgggc cggggccgcg
    11281 cgagaagtcg gtctcgccgt cggtcagctt ggcgaggtcg aggatcgagc gcagcggcac
    11341 gtactcgccg aacggcacca ggcgcgcctt gtcgtaggcg gcgacgatgc ggccggctcc
    11401 gtcgaccgcc tcaaggctgt tccagacgcg gaagccgtgg tcgccggtct cgaccctgag
    11461 cgcgccggtg atcagcgccc cgcctagcgg caccacggcg gcgagcgccg cgcgcaccgc
    11521 gccgtcctcg gccaacacgt aaggggtcgc ggtctcgggc cagatcatgt ggctcggcgg
    11581 cggcacgccc ggcagcgccg acagctcgag ctgcttggcc aggtggcgct cgcgcagcgc
    11641 gggctgccat ttgtggaact gggagatgtt ggcctggacc aggcgcagct cgacccccgg
    11701 cacctcggcc acctcggcga tgccaagccg caccgccccg cccgcgtgcg cgagcgcgac
    11761 cgccgcccag gccgcggcca ccgcccgcca ggcgccgcgc gcctcggcga gctgcgccgg
    11821 cagggcggcg gcgagcacgg cgagccagct cagcccgaac acgccgaaca gcgcggcgag
    11881 ctgaatgccg gcgccggacg gcatccacac gtagccggcg aggttccacg ggaagccgcc
    11941 gaacagggcg aaggcgcgca gccattcggc gccggtccac gccgtcgcca gcaccaccac
    12001 cccggccacc ccggtcgcgc ccgagacgcg cgcagccagc agggcgaggc cgggaaagac
    12061 ggcgcaggtg gcggccagcg cgaccaccgc gaacggcgcc agccaggtcg acaccccggc
    12121 cggcgcgaag gcgttgccga cccagtacag gccgctcagg aaatggccga ggccgaacca
    12181 gaagccgagg gcgaaccagc cggtccgggt cgcgcttccg gcggcgagcc agaccaggcc
    12241 ggagaacgcc accaccgcca gcggcaccag cgcgagcggc ggcaaggcga gggtcgcgca
    12301 cagcccgagc aggcccgcgg ccagcgcccg ccgccagccg ccgagcgcgc cgagatgccg
    12361 tgccgcgccc gcgagcaggg ttggcggtgg ccggctcacc cgtgcgcggc gggggcgtcg
    12421 gcctggccgg cctggccgcg gacgatgcgc acccggcgga cccgccgcgg gtcggcgtcg
    12481 aggatctgga tgtcgagccc ggccggatgg gagatcacct cgcccgccgc cggcacccgc
    12541 ccggcgatgt cgaacaccag gccgccgacg gtgtcgatct cctcgtcgcg gtcggccggc
    12601 aacaggtcga tgccgagcac cggctcgagg tcctcgatca gcgcccgcgc gtcggcgacc
    12661 acgctgccgt cggcgcgctc gacgatcgcc ggcgccaccg cccggtcgtg ctcgtcctcg
    12721 atctcgccga cgatctcctc gaccaggtcc tcgatcgtca ccaggccgtc gatgccgcca
    12781 tgctcgtcga ccaccagcgc catgtggagc ttgctcgcgc gcatctggcg cagcagcgtc
    12841 tgcaccggca tcgacggcgg cacgaacggc agcggccgca tcgccttggc gagcgagaac
    12901 gcaccgtggt cgtgccagaa ggcgatcagg tcgcggacgt ggatcatgcc caggatgtcg
    12961 tcgaggctgt cgcggtagac cggcaggcgc gaatggccgg cctcggacat cacccggatc
    13021 acctcggcca aggtcgcggt ctcgggcacg gcgacgatgt cggcgcgcgg caccatcacg
    13081 tcctcgaccc tgaggccgcc gaaggcgagc aggttgagca gcatcacccg ctcctgctcg
    13141 tcgatggcga ttcccgattc ccggtactgc tggatcagcc gctcgaccgc ttcgcgcaag
    13201 gacgtgcggc gctcgccgag cccggtcagc gagcgcagcc agtcgccgat caccgacacc
    13261 agcgcgcggt cgtccgcctc gcgcctctgc cggcccgacg gccgcggcgg gcgaaggtcg
    13321 ttctcggtca tgccacgtcc cgcggcaggc ggtagggatc ggcgatgccg aggctggcga
    13381 gcgccgccgt ctccagcgct tccatggcct cggccgcggc tgaggcgtgg tgatcgtggc
    13441 cgaccagatg gagcacgccg tgggccacca ggtgggccat gtggtcggcc acgtccttgg
    13501 cctgcgcccg cgcctcgcgc accaccgtct cgcgcgccag caccacgtcg ccgagcaggc
    13561 gcggcgcgcc ggcggccgcg gccccggcat ccgccatcgg gaacgccagc acgttggtcg
    13621 cgccctcttt gccgcggtag cgggcattga gctcggcgac gcggccgtcg tcggcgagca
    13681 cgatgctcac ctcgcccgcc tcgcccgctt ggcgaagggc cgcgcgcgcg gcacgcgcgc
    13741 acagcgcggc ggcatcgggc accgcgctca gccagccgtc gtcggcgatc gcgaccgcga
    13801 cctcgtcgcc gctgccggca ggcccgctca ccgcggccgc tcccggtcct cggccgcgcc
    13861 ggggcgcccg ttgcgctcga actcgtcata ggcgcggacg atgcgggtga ccagctcgtg
    13921 gcggaccacg tcggcgtcgg tgaaccggac cacctcgacg ccgtcgatgt cggcggtcac
    13981 caccagcgcg tccttgaggc ccgagcggtt gccgaccggc aggtcgacct gcgagggatc
    14041 gccggtcacc accatgcgcg agttctcgcc cagccgcgtc aggcacatct tcatctgcac
    14101 cggggtggtg ttctgcgcct cgtccaggat gacgaaggcg ttggccagcg tgcgcccacg
    14161 catgaacgcc agcggcgcga tctcgatctc gccgctctgg atgcggtcga cgacacgttc
    14221 ggcgggcatc atgtcgtaca gcgcatcgta gagcgggcgc aggtacggat cgaccttttc
    14281 gcgcatgtcg ccgggcagga agccgagccg ctcgccggcc tcgaccgcgg gccgcgacag
    14341 gatgatcctg tcgacctggc cgcgcgccag catcgacgcc gccaccgcca ccgcgagata
    14401 ggtcttgccg gtgccggccg ggccgatgcc gaacaccagc tcgtggtcgt agatcgcgcg
    14461 cacataggcc gcctgcatcg gcgagcgcgc catgatccgg cgcttctggg tggtgacgac
    14521 gctgtcctcg gcactcagcc gcgccggccc gggcgccccg gcgtcgcggc cggcggccat
    14581 gcgcagcacc ccgtcgacct cggcggtgcc gaccggaatg ccgcgcgcga gcatgtcgta
    14641 gagctcgctc agcacccgct tggcgcgctc ggcggcatcg ggccggccgg tgatcgccac
    14701 ccggttgccg cggctcgcca cccacacgcc gagcttctgc tcgatgcgcg cgaggttgcg
    14761 gtcgtgctcg ccgtagagcg cgcccaacag cgcgttgtcg tcgaaggcaa ggtaaagcgg
    14821 cgtcgcgtcc ttggccgcgt cggtggcgcc ggcgggacgg gtcacgcgcg tccttccgcc
    14881 gccggcgcgg caaccacctc gcccaacagg ctgttggcga gcacggcggt gatgcggacc
    14941 tcgacgacgt cgccgatggc ggccgcgggg gcggcgaggt ggaccggctg caagtacggg
    15001 ctgcgcccgg cgagctggcc gggatgccgc ccgcgcttct ccagcagcac cggcagcgtc
    15061 cgggcgaccg tggcttggtt gaacgcccgc tgctggtcgg cgagcagcgc ctgcagcgcg
    15121 gtcaggcgcg cgcgcttctc ggcctcgggc acctggccgg ctgcggcggc cgcgggcgtg
    15181 cccggacggg cgctgtactt gaacgagaac gcggccgcga agccgatctc ctcgaccagg
    15241 gcgagcgtgt cgtcgacgtc gcgcgcggac tcgcccggga agccgacgat gaagtcggac
    15301 gacagcgcga ggtcggggcg cgccgcgcgc agctcggcga cgatgcgccg gtagcggtcg
    15361 gcgtcgtggc cgcggttcat cgccgccagc acccggtcag agcccgactg gaccggcagg
    15421 tgcaaatagg gcatcagcgc cggcacgtcg cgatgggcgg cgatcagctc agggtccatg
    15481 tcgcgcgggt gcgaggtggt atagcgcagg cgccgcaagc cctcgatccc ggccaggcgc
    15541 ccgatcagcc gggcgagccc ccaggcgcgc ccgtccgggc cctcgccgtg ataggcgttg
    15601 acgttctggc caagcagcgt gatctcgcgc gcgcccgccg cgaccaggcg ctcgacctcg
    15661 gcgaccacct gggcgaccgg gcgcgagtac tcggcgccgc gggtgtaggg caccacgcag
    15721 aaggtgcaga acctgtcgca gccctcctgg accgagacca aggcggcaac cccgccggcc
    15781 gcgcgcgcct cgggcaggtg gtcgaacttg gcctcgaccg ggaacgaggt atcgagcacc
    15841 ggcgcgccgg cggcgcggtg cgcccgcgcc accatctccg gcagccggtg gtaggtctgg
    15901 gggccgagca cgatgtcgac gaacggcgcc cggcggatca cctcggcgcc ctcggcctgg
    15961 gcgacgcagc cggcgacggc caggatcatg cgcccgccgg cggcggcctt ctccgccttc
    16021 agcggccgca gccggccgag ctcggaatag accttctcgc tcgccttctc gcggatgtgg
    16081 caggtgttga ggatgaccat gtcggcatcc tcggcacgcg cgaccggcgc gtagccgagc
    16141 ggtgccagca cctccgccat gcgcgccgag tcgtaggcgt tcatctggca gccgtaggtc
    16201 ttgaggtaaa gcttgttggc ggccggcgca cgcgccctcg ccgcggcctt ccggccggcc
    16261 gtcgccgcag gcccagggtc agggtgatgc acggtcagtc ggtttcgcag cctcgctcac
    16321 gccacggccc cggcgtcgga tcgcccgccg gccggtccgg ccgaccagca tccgctaaac
    16381 ctttccagcg acacgggtga atgcctcggc acggaccgtc gcgatgggcg aagcccggac
    16441 ggcgcgggcc ctacgtccct cactctagcg ccggcgccaa ggctgtcaag cggcgcccgg
    16501 cggcggtagt ggctgaatta ggacaaccgg accagtaaat taggacaaac tccctcgctc
    16561 agacactcgg agtagagccc tgaactcatc ctttcgaccc gctatttggt cgttcaactc
    16621 aatcagctcg tggagcacag cagtgaagct gaggtgtacg acgaacacga tccatgcggc
    16681 gatgccagcc agcacgtaga tcgtcgcaac cccctgccag cctgcaatgg ctaccccggc
    16741 tgccagtatc cccgtcaaca cccagaactg ccaaactcga ccccaaaagg cgatccgctc
    16801 ttgcagctta tccgcctcta agtgtccgtc cggggagttt gtgagtcggg cgaatcggtt
    16861 ggtcgaattg accccttgaa gcgcgggact cgggtgtgat tcgctccggg cattgatctt
    16921 cctgccgcga ggtgcccgat gtgga
//
LOCUS       DASWHU010000317        10466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_182172, whole genome shotgun sequence.
ACCESSION   DASWHU010000317 DASWHU010000000
VERSION     DASWHU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182172"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VGB88_11800"
     CDS             complement(<1..234)
                     /locus_tag="VGB88_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9648176.1"
                     /translation="MDRFPDSLIAFQRMFPDDHACAAWLASVRWPEGFVCPRCGHDHG
                     WALGAKAHTFECARCHRQTSVTAGTLLHGSKLDL"
     gene            840..1943
                     /locus_tag="VGB88_11805"
     CDS             840..1943
                     /locus_tag="VGB88_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9648177.1"
                     /translation="MALVLAGAAGAARAQEAAPVGVDAVRVEPLDQTQPVIGRFVARQ
                     AGEVAARTAGSITALPVQVGDRVKTGDVIAALDVERLTWQRDLAASTAREMEALLDSA
                     ESQLVKRRQELARLEGIRQSAAFNQARYEDAVQDLATGEAAVAAAGAVLARVRANLRI
                     AEDDLRHGRVLAPYDGVISRRLTEVGAYVQVGDPVASMINEDDLEVEADVPYNRMAGM
                     QPDTVVTIELADGSSHNAVVRAIGAEENPMTRTRQVRFTPAVEGARVKNAVGESVAVG
                     LPLGAPREVVTVHKDAILKRQGMSLVYVVGEDDTAQVRPVELGEAVGPRFEVLGGVAA
                     GEMVVVRGNERLRPGQAVAVTADEAASAEGASQ"
     gene            1940..5137
                     /locus_tag="VGB88_11810"
     CDS             1940..5137
                     /locus_tag="VGB88_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9648178.1"
                     /translation="MSVIQGSINRPIAVIAAVLMVIIFGVLALQRIPIQLAPDVSKPV
                     VSVTTYWPGGSPLEVEREIVTRQEEKLKGIEGLVEMESSAQDGRAEITLEFEIGANMD
                     RALLLVANRLDQVERYPDEALRPTISTSSLGDNPVAWFILTRTAGNERPVASYGDFVE
                     DVIQERLERVPGVSLTNVYGGTEREMRIVIDPDLMARYRLTVPDVVQSLRAANASISA
                     GDVDEGKRRYVVRAEGEFETVDQVRQVVLTSQESAATGRIGRVTVGDIADVRFDYKDS
                     TAHIRFLGEDSLAVNAVRETGANVIETMKGIDQAVGELNQYALPQAGLRMRQVYDETV
                     YIKSSIALVRQNIWVGGTLAALILLLFLRSLRATLIVSLAIPISVVAAFVAMAALGRS
                     INVISLAGIAFAVGMVVDAAIVVLENIYRYRERGLAPIEAAYKGTKQVWSAVLVSALT
                     TVLVFIPILVMELEAGQLFRDIAVAISVAVLASLVVSITVVPALSKTLLKNVNISESR
                     LRLPVVDLFARLFAGLIMGYARTIIRSRVASLIVVGALVAGTAVASLEFLPKLEYLPE
                     GNRNLIFGIMLPPPGYNLDTMAEIAGRVEGRVRHLWGSETGPEPEPGGPPKIRNYFFV
                     ALPNRAFFGAGTYDETSDRVGELIPILSQSLWGEPGTFGFFTQPSLFSRDIAGGRAIK
                     LDISGPDLEDVVVTSQKAAGLVEAAYPRAQGNQMRPVPGLELGSPEVRVLPDRVRLAD
                     NGVSALALGQSIDAFNDGLRVAEITVDGRRTDLTLRGPENKIDRTQGIDNLPVVTASG
                     QILPVSMLANVVITTGPTEVRHLERERTITLEIRPRNEVSLEEAIDTVQTQVIDKLRA
                     EGLPAGVRVRMSGSAAELDVTFDAMVNNLVVAIVIVYLLMAVLFESFVYPFIIMLSVP
                     LATAGGVLGLVVLNIYMSRSGGVQALDMLTMLGFIILVGIVVNNAILLVDQTLKLMRE
                     EGMASFDAICEATRNRLRPIFMSTLTSVFGMLPLVVFPGAGSELYRGLGSVVVGGLSL
                     SAILTLLIIPPLLAIFLPKPEAAAAETVALRAAAE"
     gene            complement(5183..5503)
                     /locus_tag="VGB88_11815"
     CDS             complement(5183..5503)
                     /locus_tag="VGB88_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9648179.1"
                     /translation="MAKLVEMRTYRLKPGGPAQWMKTYEEQALKLQKEILGHLVGYYQ
                     TEIGPLNEVIHMWAYDGLDARAERRAKLASHPDWQKFIPNVRPLVETQESKILIPAPF
                     SPTP"
     gene            5646..6638
                     /locus_tag="VGB88_11820"
     CDS             5646..6638
                     /locus_tag="VGB88_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9648180.1"
                     /translation="MAETYRALVVDKAGEGTEVALVERSADELPAGEVTIRVAYSSVN
                     YKDGLATLAKGGVVRKWPHVPGIDLAGTVAASRDPRFAEGDEVLVTGYDLGVAHDGGF
                     AEFARVPADWVVKRPDGLSLRECMAFGTAGFTAALALDALDRHGVTPAAGPAIVTGAT
                     GGVGSIAVSMLSGLGFEVAASTGKAGEHDYLRSLGASRILSRDEVSAETRPLDAAMWA
                     AAVDQVGGSTLPWLMARMTYRGAIASTGLTGGPAFKATVLPFLLRSVAILGIDSVFCP
                     MAERLVLWQRMADDLKPPHLTDMIAAETGLDGVKDVLAGILKGGVRGRTVVRIA"
     gene            complement(6650..7228)
                     /locus_tag="VGB88_11825"
     CDS             complement(6650..7228)
                     /locus_tag="VGB88_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648181.1"
                     /translation="MTGAKAQPAVLVALAGAGGVAPALRKAWAASWSYRIARNRGRAD
                     AFSDTRVVARFPGAVRAVRCALAVEADLALFAQRLSRPADLALRIGVAIGAADMAAAL
                     AELAPAAGLAVSGAVRAEVEGKLDLDFAALDGVPADSGAVAVTSALKQRVFRFKPWTT
                     PGRRSWGLIAAALVLAAVVIGLALSLPPAGAP"
     gene            7285..8145
                     /locus_tag="VGB88_11830"
     CDS             7285..8145
                     /locus_tag="VGB88_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9648182.1"
                     /translation="MLYRDFATVEDLDREYNLQLRVPEAPAIIERWARESEAARAKLG
                     GALGVRFGPTVDEYLDIFPATGGGKRPVHLFIHGGYWRRFTAREFSFVAPRLVAAGVC
                     VAVSNYALCPKVSVTEIVRQTRAALAWLHGHAADYGGDAGRITVSGHSAGGHLTALML
                     ATDWPGDYGLPAELIKGACPISGVHDLRPVPYTFIQPSVQLGWDEARRLSPIDLIPAS
                     APPITVAVGGGETDEFVRQSRAYAEACRAAGLGCAYLEIDGAHHFDILDGFTDPGSLL
                     FKAILALTGA"
     gene            complement(8153..8944)
                     /locus_tag="VGB88_11835"
     CDS             complement(8153..8944)
                     /locus_tag="VGB88_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX9648183.1"
                     /translation="MIVKQIYTDNAYRNFNYLIACPDTGAALAIDPLDHRKCLAAAKA
                     EGWDITQILNTHEHGDHTGGNRVMIEATGAKLLAHKGATGKIPGIDVGLSAGDVIKVG
                     KTVELEVLDTPGHTMSHVCVLSHTDRPALLCGDTLFNAGAGNCHNGGHPEELYHTFAG
                     QLARLPDQTRVFPGHDYIANNLAFTLDREPDNARARVLLAEVKDQDPHHARVTTLALE
                     KEINTFFRLHSPSVITRLREAVPGFPDAPTPKQVFLALRALRNTW"
     gene            complement(9424..9852)
                     /locus_tag="VGB88_11840"
     CDS             complement(9424..9852)
                     /locus_tag="VGB88_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9648184.1"
                     /translation="MSYLIDTNIISEVRKGPRCDAAVAAWYASIDDADLYLSVLVLSE
                     IRNGIERARPREPAKAEALDRWLTEVASAFGERVLAIDRAVADDWGRMMALRPLPVID
                     SLLAATAKAHRMTLVTRNDADVSGLGAKLLNPFKPAAAGG"
     gene            complement(9849..10133)
                     /locus_tag="VGB88_11845"
     CDS             complement(9849..10133)
                     /locus_tag="VGB88_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648185.1"
                     /translation="MADTRETPVTRHRRLMKRQGVVRVEVQVRKEDASLVRSVARALT
                     DPEREADARALLRQRFAEPRAKGLKALLAAAPLDGIDIERVRDTGRMVEL"
     gene            complement(10262..>10466)
                     /locus_tag="VGB88_11850"
     CDS             complement(10262..>10466)
                     /locus_tag="VGB88_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9648186.1"
                     /translation="PFREVRRRLLAAGWVEVAATGSHVKFAKRSAAGTRTAIVPKHRE
                     IARGTLRSSPRQAGISPEAFDRM"
BASE COUNT         1529 a         3674 c         3671 g         1592 t
ORIGIN      
        1 gagatcgagc ttgctgccat gcaggagggt gcccgccgtc accgaggtct ggcgatggca
       61 gcgggcgcat tcgaaggtgt gcgccttggc gccgagcgcc cagccatgat catggccgca
      121 gcgcgggcag acgaagccct ccggccaacg caccgacgcc agccatgccg cgcaggcgtg
      181 gtcgtcgggg aacatgcgtt ggaaggcgat caggctgtcg gggaagcggt ccatggcgcg
      241 gctcctggcg ttggaacaaa ccaggaacat gctaccgact caccgccctc aacgcaacat
      301 gtcgtgcggc cggagcgtcg tgcataagcg actacctgta atcgatcggg aacgcgcgtt
      361 cgtttacttt agactcttcc cgacgtttat ttcggacata gagtcttttg cgaattattg
      421 cggcacgcat gaaatatagg ttccccggca tcaccaaatg gcgaccgcct atgcactata
      481 cgttaacacg aaaaactcgg aagtgttgcc acttttggcc gacaaatgga tgtctcctcc
      541 tagatattga ccgtcaagac agtcgccact ggtggcggac cggtttgcga ttcaagagtt
      601 gtgaagtcga gccagcactc ctgaacgggc gcctcaagtg ggtcgctgac ccccgcttct
      661 aggccgaggc accgccgccg cggggtcccg cttccgattc gaacggttgt ttgataccgt
      721 gccgcgccgg cctggggcgc gtgtgacctt gttcacagtg cggccccggg gccggggcgg
      781 agagtagggc gatgacggtt gcgcgggtgg gcgcggtgcg ccgctctctg gcgttggttg
      841 tggccctggt cctggccggg gcggcggggg cggcgcgcgc gcaggaggcg gcgccggtgg
      901 gcgtggacgc ggtgcgcgtc gagccgctcg accagaccca gccggtgatc ggccgcttcg
      961 tcgcccgcca ggcgggcgag gtggcggcgc gcaccgccgg ctcgatcacc gccctgccgg
     1021 tccaggtcgg cgaccgggtc aagaccggcg atgtgatcgc cgccctcgac gtcgagcgcc
     1081 tgacttggca gcgcgacctc gctgcctcga ccgcgcgcga gatggaggcg ctcttggaca
     1141 gcgccgagtc gcagctggtc aagcgccgcc aggagcttgc gcgcctggaa ggcatccgcc
     1201 agtcggcggc gttcaaccag gcgcgctacg aggacgcggt ccaggacctc gccaccggcg
     1261 aggccgcggt cgccgcggcc ggcgcggtgc tcgccagggt gcgcgcgaac ctcaggatcg
     1321 ccgaggacga cctccgccac gggcgtgtgc tcgcccccta tgacggggtg atcagccgcc
     1381 gcctcaccga ggtcggcgcc tacgtccagg tcggcgaccc ggtggcctcg atgatcaacg
     1441 aggacgacct cgaggtcgag gccgacgtgc cctacaaccg catggccggc atgcagccgg
     1501 acacggtggt gaccatcgag ctcgccgacg gctcgagcca caacgcggtg gtgcgcgcga
     1561 tcggcgccga ggagaacccg atgacccgca cccgccaggt ccggttcacg ccggcggtgg
     1621 aaggggcgcg ggtcaagaac gcggtcggcg agtcggtcgc ggtcgggctg ccgctcggcg
     1681 ccccgcgcga ggtggtgacc gtgcacaagg acgcgatcct caagcgccag ggcatgtcgc
     1741 tggtctacgt ggtcggcgag gacgacaccg cccaggtgcg cccggtcgag ctcggcgagg
     1801 cggtggggcc gcgcttcgag gtgctcggcg gcgtcgccgc gggcgagatg gtggtggtgc
     1861 gcggcaacga gcggctcagg ccgggccagg cggtggcggt caccgcggac gaggccgcga
     1921 gcgccgaagg ggccagccaa tgagcgtcat ccagggctcg atcaaccgcc cgatcgcggt
     1981 catcgcggcg gtgctgatgg tgatcatctt cggcgtgctg gcgctccagc gcatcccgat
     2041 ccagctcgcg ccggacgtga gcaagccggt ggtcagcgtc accacctact ggccgggcgg
     2101 ctcgccgctc gaggtcgagc gcgagatcgt cacccgccag gaggagaagc tcaagggcat
     2161 cgagggcctg gtcgagatgg agagctcggc gcaggacggg cgtgccgaga tcacgctcga
     2221 gttcgagatc ggcgccaaca tggaccgcgc gctgctgctg gtcgcgaacc gcctcgacca
     2281 ggtcgagcgg tacccggacg aggcgctcag gccgaccatc tcgacctcga gcctgggcga
     2341 caacccggtc gcctggttca tcctcacccg caccgccggc aacgagcgcc cggtcgcgag
     2401 ctacggcgac ttcgtcgagg acgtgatcca ggagcggctc gagcgcgtgc cgggcgtgtc
     2461 gctgaccaac gtctacggcg gcaccgagcg cgagatgcgc atcgtcatcg atcccgacct
     2521 gatggcgcgc taccgcctga ccgttcccga cgtggtgcag tcgctccggg cggccaacgc
     2581 ctcgatctcc gccggcgacg tcgacgaggg caagcgccgc tacgtggtcc gcgccgaggg
     2641 cgagttcgag accgtcgacc aggtccgcca ggtggtgctg accagccagg agagcgcggc
     2701 caccgggcgc atcggccggg tcaccgtcgg cgacatcgcc gacgtgcgct tcgactacaa
     2761 ggactcgacc gcgcacatcc gcttcctggg cgaggactcg ctggcggtga acgcggtgcg
     2821 cgagaccggc gccaacgtga tcgagaccat gaaggggatc gaccaggcgg tcggcgagct
     2881 caaccaatac gcgctgccgc aggccggcct caggatgcgc caggtctacg acgagaccgt
     2941 gtacatcaag tcctcgatcg cgctggtgcg ccagaacatc tgggtcggcg gcacgctcgc
     3001 ggcgctgatc ctgctgctgt tcctgcgctc gctcagggcg acgctgatcg tgtccttggc
     3061 gatcccgatc tcggtggtgg cggcgttcgt cgccatggcc gcgctgggac ggtcgatcaa
     3121 cgtcatctcg ctcgccggca tcgcgttcgc ggtcggcatg gtggtcgacg cggcaatcgt
     3181 ggtactggag aacatctacc gctatcgcga gcgcggcctg gcgccgatcg aggccgccta
     3241 caagggcacc aagcaggtgt ggagcgcggt gctggtctcc gcgctgacca cggtgctggt
     3301 gttcatcccg atcctggtca tggagctcga ggccggccag ctgttccgcg acatcgcggt
     3361 cgccatctcg gtcgccgtgc tcgcctcgct ggtggtctcg atcacggtcg tcccggcgct
     3421 gtcgaagacg cttctgaaga acgtcaatat ctccgagtcc aggctcaggc tgccggtggt
     3481 cgacctgttc gcgcgcctgt tcgcgggcct gatcatgggc tatgcgcgga ccatcatcag
     3541 aagccgcgtc gcctcgctga tcgtggtcgg ggcgctggtc gccggcaccg ccgtcgccag
     3601 cctcgagttc ctgcccaagc tcgagtacct gcccgagggc aaccgcaacc tgatcttcgg
     3661 catcatgctg ccgccgccgg gctacaacct cgacaccatg gccgagatcg ccggccgggt
     3721 ggaggggcgc gtgcgccacc tgtggggcag cgagaccggg cccgagcccg agcccggcgg
     3781 gccgcccaag atccgcaact acttcttcgt cgcgctgccc aaccgcgcct tcttcggcgc
     3841 cggcacctac gacgagacct cggaccgggt cggcgagctg atcccgatcc tcagccagtc
     3901 gctgtggggc gagcccggca ccttcggctt cttcacccag ccgagcctgt tcagccgcga
     3961 catcgccggc gggcgcgcga tcaagctcga catctcgggc cccgacctcg aggacgtggt
     4021 ggtcacctcg caaaaggcgg cgggcctggt cgaagcggcg tacccgcgcg cgcaaggcaa
     4081 ccagatgcgc ccggtgcccg ggctcgagct cggctcgccc gaggtgcgcg tgctgcccga
     4141 ccgggtgcgg ctcgccgaca acggcgtctc tgcgctcgcg ctcggccaga gcatcgacgc
     4201 cttcaacgac ggcctgagag tcgccgagat caccgtcgac ggccggcgca ccgacctcac
     4261 gctccgcggc ccggagaaca agatcgaccg cacccagggc atcgacaacc tgccggtggt
     4321 gaccgcgagc ggccagatcc tgccggtgtc catgctcgcc aacgtggtca tcaccaccgg
     4381 cccgaccgag gtgcgccatc tcgagcgcga gcgcaccatc accctcgaga tcaggccgcg
     4441 caacgaggtc tcgctcgagg aggcgatcga cacggtgcag acccaggtga tcgacaagct
     4501 gagggccgag ggcctgccgg cgggggtgcg cgtgcgcatg tccggctccg ccgccgagct
     4561 cgacgtcacc ttcgacgcca tggtcaacaa cctggtggtc gccatcgtca tcgtctacct
     4621 gttgatggcg gtgctgttcg agagcttcgt ctatccgttc atcatcatgc tgtcggtacc
     4681 gctggcgacc gcgggcggcg tgctcgggct ggtcgtgctc aacatctaca tgagccggtc
     4741 cggcggcgtg caggcgctcg acatgctgac catgctcggc ttcatcatcc tggtcggcat
     4801 cgtcgtcaac aacgcgatcc tgctggtcga ccagacgctc aagctgatgc gcgaggaggg
     4861 catggcctcg ttcgacgcga tctgcgaggc gacccgcaac cggctcaggc cgatcttcat
     4921 gtcgaccttg accagcgtgt tcggcatgct gccgctggtg gtcttccccg gcgccggctc
     4981 cgagctctat cgcggactgg gctcggtggt ggtcggcggg ctgtcgctgt cggcgatcct
     5041 gacgctgctg atcatcccgc cgctgctcgc gatcttcctg cccaagcctg aggccgccgc
     5101 cgccgagacg gtggcgctgc gcgcggccgc cgagtagccg cgcccggccg cgacgcgagc
     5161 cgccgcggcc gggcacgccg gatcagggcg tcggcgagaa cggcgccggg atcaggatct
     5221 tcgactcctg ggtctcgacc agcggccgga cgttggggat gaacttctgc cagtcggggt
     5281 gcgaggcgag cttggcgcgg cgctcggcgc gcgcatccag gccgtcatac gcccacatgt
     5341 ggatgacctc gttcaacggc ccgatctcgg tctggtagta gccgaccaga tggccgagaa
     5401 tctccttctg cagcttgagc gcctgctcct cataggtctt catccactgc gccggcccgc
     5461 cgggcttgag ccgataggtg cgcatctcga ccagtttggc catttgctcc ctcccaatga
     5521 aatgatcgcg ccgcggatcg gcgcctgagt gcgcggccac gaaaccagcc gacccgaccc
     5581 ttgtcaacgc gcgcaaggaa ggactagaca gtcaccgcgc gcgcaagcct gagggaggga
     5641 cgatcatggc cgagacctac cgcgcgctcg tcgtcgacaa ggcgggcgag ggcaccgagg
     5701 tcgcgctcgt cgagcgcagc gccgatgagc tgcctgcggg cgaggtgacg atccgcgtcg
     5761 cctattccag cgtcaactac aaggacggcc tggcgacgct cgccaagggc ggcgtggtgc
     5821 gcaagtggcc gcacgtcccc ggcatcgacc tcgccggcac tgtggccgcc tcgcgcgatc
     5881 cgcgcttcgc cgaaggcgac gaggtcctcg tcaccggcta cgacctcggc gtcgcccatg
     5941 acggcggctt cgccgagttc gcgcgcgtgc ccgccgactg ggtggtcaag cgccccgacg
     6001 gtctcagctt gcgcgagtgc atggcgttcg gcaccgccgg cttcaccgcc gcgctcgcgc
     6061 tcgatgcgct cgaccgccac ggggtgacgc ccgcggcggg cccggcgatc gtcaccgggg
     6121 cgaccggcgg ggtcggctcg atcgcggtca gcatgctgag cgggctcggc ttcgaggtcg
     6181 cggcgtccac cggcaaggcg ggtgagcacg actacctgag aagcctcggc gcgagccgga
     6241 tcctgtcgcg cgacgaggtc tcggccgaga cccggccgct ggatgcggca atgtgggcgg
     6301 cggcggtcga ccaggtcggc ggcagcacgc tgccgtggct gatggcgcgc atgacctatc
     6361 gcggcgcgat cgccagcacc gggctcaccg gcggcccggc gttcaaggcc accgtgctgc
     6421 cgttcctgct gcgcagcgtg gcgattctcg gcatcgactc cgtgttctgt ccgatggccg
     6481 agcgactggt gctgtggcaa cggatggcag acgacctgaa gccgcctcat ctcaccgaca
     6541 tgatcgcggc cgagaccggc ctcgacggcg tcaaggacgt gctcgccggc atcctcaagg
     6601 gcggggtgcg cgggcgcacg gtggtcagga tcgcctgaca gggcgccgct cagggcgcgc
     6661 ccgccggcgg cagcgacaaa gcgagcccga tcaccaccgc ggcgagcacc agcgccgccg
     6721 cgatcaggcc ccagctgcgc cggccgggcg tggtccacgg cttgaaccgg aacacgcgct
     6781 gtttgagcgc gctggtgacc gcgaccgcgc cgctatccgc cgggacgccg tcgagcgcgg
     6841 cgaagtcgag atcgagcttg ccctcgacct cggcgcggac cgcgcccgag accgcgagcc
     6901 cggccgccgg cgcgagctcg gcgagggctg ctgccatgtc cgccgcgcca atggcgacgc
     6961 cgatgcgcaa ggcgaggtcc gccggccggg acaggcgctg cgcgaacaag gcgaggtcgg
     7021 cctcgaccgc gagcgcgcag cgcaccgcgc gcactgcgcc cggaaagcgc gccaccacgc
     7081 gcgtgtccga gaacgcgtcc gcccggccgc ggttgcgcgc gatgcggtag ctccacgacg
     7141 ccgcccacgc cttgcgcagc gccggcgcga cgccgccggc gccggcgagc gcgaccagga
     7201 ccgcgggctg cgccttggcg cccgtcatcg accgcggcgc cgcgctcggc tatgactggc
     7261 gccggccaac cggggaggac cgccatgctc tatcgcgact tcgccaccgt cgaggacctc
     7321 gaccgcgagt acaacctgca actgcgcgtg cccgaggcgc cggcgatcat cgagcgctgg
     7381 gcgcgcgagt ccgaggccgc gcgcgccaag ctcggcggcg cgctcggcgt ccgcttcggg
     7441 ccgacggtgg acgagtacct cgacatcttc ccggccacgg gcggcggcaa gcggccggtg
     7501 cacctgttca tccacggcgg ctactggcgc cgcttcacgg cgcgcgagtt ctcgttcgtc
     7561 gccccgcgcc tggtcgcggc cggggtgtgc gttgcggtca gcaactacgc gctgtgcccg
     7621 aaggtgtcgg tgaccgagat cgtgcgccag acccgcgccg cgctcgcctg gctccacggc
     7681 catgccgccg actatggcgg cgatgccggc cggatcacgg tctcggggca cagcgccggc
     7741 ggccacctga ccgcgctgat gctggccacg gactggccgg gcgactacgg cctgcccgcc
     7801 gagctgatca agggcgcgtg cccgatcagc ggcgtccacg acctccgccc ggtgccctac
     7861 accttcatcc agccgtcggt gcagctcggc tgggacgagg cgcgccggct gtcgccgatc
     7921 gacctgatcc cggcctccgc gccgccgatc acggtcgcgg tcggcggcgg cgagaccgac
     7981 gagttcgtgc gccagagccg cgcctacgcc gaggcgtgcc gggcggcggg cctcggctgc
     8041 gcgtatctcg agatcgatgg cgcccaccat ttcgacatcc tggacggatt caccgacccc
     8101 ggcagcctgc tgttcaaggc gatcctggcg ctgaccggag cctgagcgcg cctcaccatg
     8161 tgttgcgcag cgcgcgcaac gccaggaaca cctgcttcgg cgtcggcgcg tcgggaaagc
     8221 cgggcaccgc ctcgcggagc cgcgtgatca cgctcgggct gtgcaggcgg aaaaaggtgt
     8281 tgatctcctt ctcgagcgcg agcgtggtca cgcgcgcatg gtgcggatcc tggtccttga
     8341 cctccgccag cagcacgcgg gcgcgcgcat tgtcgggctc gcggtcgagg gtgaaggcga
     8401 gattgttggc gatgtagtcg tggccgggaa agacccgcgt ctggtcgggc agcctcgcga
     8461 gctggccggc gaaggtgtgg tacagctcct cggggtggcc gccgttgtgg cagttgccgg
     8521 cgccggcgtt gaacagcgtg tcgccgcaca ggagcgccgg ccggtcggtg tgcgacagca
     8581 cgcagacgtg gctcatggta tggcccgggg tatcgagcac ctcgagctcg accgtcttgc
     8641 cgaccttgat cacgtcgccc gcggaaagcc ccacgtcgat gcccgggatc ttgcccgtgg
     8701 cgcccttatg cgcgagcagc ttggcgccgg tggcctcgat catcacccgg ttgccgccgg
     8761 tatggtcgcc gtgctcgtgg gtgttgagga tctgggtgat gtcccagcct tcggccttcg
     8821 ccgccgccag gcacttgcgg tggtcgagcg ggtcgatggc cagcgccgcg ccggtgtccg
     8881 ggcaggcgat caggtagttg aagttgcggt aggcgttgtc ggtgtagatc tgcttgacga
     8941 tcatcggctc ctccgcggcg gccggcggca gtctagaccg ggaggctacc gaggcacaaa
     9001 gcgcgccgat tggtgaccgc cgtcacagtg ccggcgcgcg gtccgtggca caagggttgc
     9061 gcttccccgc aacaccttgg cgccgcccat tgcttcccaa tgggcggcgc ctcttttttt
     9121 tcccgcctcg ccggcgcggc gaaaaatcgc ccgcgcggtg tgaactggtt cacagcgccg
     9181 cctcaggcga cgcgccatga tggcgacgtt ccctcctcca cacactgcgc cccgcggcgc
     9241 cggtccggac tcccccccga catccccctc cggaccggcg ccgcggggga atgccgcgat
     9301 gatcgtcctt cagcccggct cctgcgcctt caccggaacc gcgacgcggc gcgccacatg
     9361 cgatcgaacg cctccgggct gatcccggcc tgacgaggac tgctgcgcaa ggtggcgcgc
     9421 ggatcagccg ccggccgcag cgggtttgaa cgggttcagc agcttggcgc cgagcccgct
     9481 cacgtcggca tcgttgcggg tgaccagcgt catccgatgt gccttggcgg ttgccgcgag
     9541 caggctgtcg atcaccggca gcggacgcag cgccatcatc cggccccagt catcggcgac
     9601 tgcacggtcg atggcaagaa cgcgctcgcc gaaggccgag gcgacctcgg tcaaccacct
     9661 gtcgagcgcc tccgccttcg ccggttcgcg cggtcgcgcg cgttcgatgc cgttgcggat
     9721 ttcgctcagc accagcacgc tcaaatagag atcggcgtcg tcgatcgatg cgtaccaggc
     9781 ggcgacggcc gcgtcgcagc gcggcccctt gcggacttcg gagatgatgt tggtgtcgat
     9841 caggtagctc acagctcgac catccgcccg gtgtcgcgga cgcgctcgat gtcgatgccg
     9901 tcgagcggcg cggccgcgag tagtgctttc agccccttgg cacgcggttc ggcgaaccgc
     9961 tggcgcagga gcgcccgcgc gtccgcttcg cgctctggat cggtcaacgc gcgggcgacg
    10021 ctgcgcacca gggacgcgtc ctccttgcgc acctgcacct cgacccgcac cacgccctgg
    10081 cgcttcatga ggcggcgatg acgcgtgacc ggggtctccc gagtatcggc catggcaacg
    10141 atcctccaac cgtttccggt aatcttaccg ggaatacggc ccaagggcaa cgttggacga
    10201 cacaaaagga tacgctcagc ccggctcctc ggccttctcc agcaccgcgc gcaacggcac
    10261 gtcacattcg gtcgaacgcc tccgggctga tgccggcctg acgaggactg ctgcgcaaag
    10321 ttccgcgggc gatctcgcga tgcttgggca cgatcgccgt acgcgtgcct gccgcggagc
    10381 gctttgcgaa cttcacgtgg ctgcccgtcg ccgcgacctc gacccaacct gccgccagca
    10441 accgccgccg gacctcgcga aacggc
//
LOCUS       DASWHU010000318         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2052428, whole genome shotgun sequence.
ACCESSION   DASWHU010000318 DASWHU010000000
VERSION     DASWHU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052428"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VGB88_11855"
     CDS             <1..1021
                     /locus_tag="VGB88_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018864395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX9648187.1"
                     /translation="ITEAAAEAMGVARANVCLFNETETELRCIDLYEAASRRHSEGEV
                     RTEAEYANEFRALKSARFVDAHDPLTDPRTAGYVERYLKPLGITAMLDAVIQVSGRNL
                     GLLCLEHVGQPHRWEQDEIAFACQLADKIALAVVLRERHESQAALRRGLEDTVLAIAA
                     TAEMRDPYTSGHERRVAELSVAIGREMGLPEHAIEGLHFGATIHDLGKMKIPAEILVK
                     PSRLSPIEYELVKGHAQAGYETLKGISFPWPVAQMVRQHHERMDGSGYPQGLKGDAIL
                     PEARILAIADTVEAMASHRPYRPGLGIDKALAEIERGRDAQYCAQAVDACLRLFREKG
                     YTLPA"
     gene            1053..1691
                     /locus_tag="VGB88_11860"
     CDS             1053..1691
                     /locus_tag="VGB88_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9648188.1"
                     /translation="MTRTLLLALLFACGPALAQNPGWYIGAGAGSTQADFVRSDFTAT
                     GLATGTYSADDSDIGIRIFGGYRVAPNWALEFGYASLGRYKHRYNNAGNVAIYDYEAS
                     ALTVAAAASLPLAGGLSLNGRVGVALTAAELGLRRDNGTANPPFCPDSWWWSSDCSSR
                     RNNLYWGLGAQFDVSPRLGIRLDYDNYGEVGEEFETGRADIETVSVNFVWRF"
     gene            complement(1688..3802)
                     /locus_tag="VGB88_11865"
     CDS             complement(1688..3802)
                     /locus_tag="VGB88_11865"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HEX9648189.1"
                     /translation="MVTNLNTFAIAGLAFTDSYPANLVNAAVPGLVNSCGGAASAVAG
                     GTQLTLNGGTIPAASSCTVSVSVTSAVAGSYNNGTGTVTTTNAGNGAPTFAVLDVAAA
                     VSSFNVVEPGGDAVTGRIFTRIAGQDIAVDIVARDASNMVAAGFTGTVAVELVDNTSG
                     GACAGLPLIKALADQTFTGGDAGRHPLSAGQFEANAWRNVLFRVKFPVAAPTVTSCSS
                     DAFANRPLQFASVLVRDQDRVTAGTTRTLNNTSNPGIGTVHNAGRPFRVDATAQNGAG
                     APATTTLYSPGAGQPVAILAQCGAGAVCPATPGTLGLGAWSAAAGVITTTTATYADVG
                     AFNLTLQDQTFASVDAGDGTATSVRYISSATLTVGRFVPDHFTLAAGAAITPRTDIGA
                     CAGSTFTYMNERMDLAFTLDARASGGALTPGYSGATLGALVLNSAASYNFGAMDAAAP
                     TPLTTRLDLGMIGSIAAAWAGGTASVVAPVALMRAAAPDGPYGMLRLGIAPADPDGVA
                     PLGAALDLDADNDATPERAQIGAATAGRFGRLVLQSVYGPSNRDLPVTLEAQYWDGTG
                     PTPGFTRNNDDGCTDFQRSDFALAFTSPPPPDPNLVACETAMVEANLTLVSGRATITL
                     ANPGAGNSGTVRLTANLDSLGGNYCPATGVAEAPATNAARAYLLGRWDDGAAYDDKPS
                     AAAGFGLYGSQPKNFIFFRENY"
BASE COUNT          570 a         1363 c         1404 g          563 t
ORIGIN      
        1 gatcaccgag gcggcggccg aggcgatggg cgtcgcgcgc gccaacgtgt gcctgttcaa
       61 cgaaacggag accgaactgc gctgcatcga cctgtacgag gcggcctcgc gccgccattc
      121 ggagggtgag gtgcgcaccg aggccgagta cgcgaacgag ttccgcgcgc tgaagagcgc
      181 gcgcttcgtc gacgcgcacg atcccctcac cgacccgcgc accgccggct acgtggagcg
      241 ctacctgaag ccgctcggca ttaccgcgat gctggacgca gtgatccagg tctcgggccg
      301 gaacctcggg ctgctgtgcc tcgagcacgt cggccagccg caccgctggg agcaggacga
      361 gatcgccttc gcctgccagc tcgccgacaa gatcgcgctc gccgtcgtgc tgcgcgagcg
      421 ccacgaaagc caggccgcgc tgcgccgggg gctcgaggat acggtgctcg ccatcgctgc
      481 caccgcggag atgcgcgacc cctatacctc gggccacgag cggcgcgtgg ccgagctctc
      541 ggtggcgatc gggcgcgaga tggggctgcc cgagcacgcg atcgaaggcc tgcacttcgg
      601 cgccacgatc cacgacctcg gcaagatgaa gatcccggcc gagatcctgg tgaagccctc
      661 gcgcctctcg cccatcgagt acgagctcgt gaagggccac gcgcaggcgg gctacgagac
      721 gctgaagggc atctcgtttc cctggccggt ggcgcagatg gtgcgccagc accacgagcg
      781 catggacggc agcggctatc cgcagggcct caaaggcgac gccatccttc ccgaggcgcg
      841 catcctcgcc atcgccgata ccgtcgaagc gatggcctcg caccgaccgt accgtccggg
      901 cctgggcatc gacaaggcgc tcgccgagat cgagcgcggc cgcgacgcgc agtattgcgc
      961 gcaagcggtg gacgcctgcc tgcggctgtt ccgcgagaag ggctatacac tgccggcgtg
     1021 acaccccagt cccttcctaa aggagactcg ccatgacaag aaccctgctg ctcgccctgc
     1081 tgttcgcctg cggcccggcg ctcgcgcaga accccggctg gtacatcggc gccggcgccg
     1141 gcagcaccca ggccgatttc gtgcgcagcg acttcaccgc cacgggcctc gccaccggca
     1201 cctactcggc ggacgacagc gacatcggga tccggatatt cggcggctat cgcgtggcgc
     1261 ccaactgggc gctcgaattc ggctacgcct cgctcgggcg ctacaagcac cgctacaaca
     1321 acgccgggaa cgtcgctata tacgactacg aggcgagcgc cctcacggtc gcggccgccg
     1381 ccagcctgcc gctcgccggc ggcctgtcgc tgaatgggcg cgtcggggtg gcgctcactg
     1441 cggccgagct ggggctgcgc cgggacaacg gcaccgccaa cccgccgttc tgcccggaca
     1501 gctggtggtg gtccagcgac tgctcttccc ggcgcaacaa tctctactgg ggcctcggcg
     1561 cgcagttcga cgtcagcccg cgcctgggca tccggctcga ctacgacaac tacggcgagg
     1621 tcggcgagga gttcgagacc gggcgcgccg acatcgagac ggtgtcggtg aacttcgtct
     1681 ggcgcttcta gtagttctcg cggaagaaga tgaagttctt cggctgcgag ccgtacaggc
     1741 cgaagcccgc cgccgcgctc ggcttgtcgt cgtaggccgc gccgtcgtcc cagcgcccga
     1801 gcaggtaggc gcgcgcggcg ttcgtcgccg gcgcctcggc cacgcccgtc gccgggcagt
     1861 agttgccgcc cagggaatcg aggttggccg tcaggcgcac ggtgcccgag ttgccggcgc
     1921 cgggattggc gagggtgatc gtcgcgcgtc ccgagaccaa agtgagattt gcttccacca
     1981 tggccgtctc gcatgcgacc aggttcgggt ccggcggcgg cggcgaggtg aacgccagag
     2041 cgaagtcgga gcgctggaaa tccgtgcagc cgtcgtcgtt gttgcgcgtg aagcccggcg
     2101 tggggccggt cccgtcccag tactgcgctt ccagcgtcac gggcaggtcg cggttgctcg
     2161 ggccgtagac gctctgcagg acaagccgcc cgaagcgccc agcagtggca gcgccgatct
     2221 gggcacgctc cggcgtcgcg tcgttgtcgg cgtcgaggtc gagcgccgcg ccgagcggcg
     2281 cgacgccgtc gggatccgcc ggcgcaatgc cgaggcgtag catgccgtag ggcccgtcgg
     2341 gcgcggccgc gcgcatgagc gccaccggcg cgaccacgct cgccgtgccg ccggcccagg
     2401 ccgccgcgat gctgccgatc attcccaggt cgagccgcgt cgtgagcggt gtcggcgccg
     2461 ccgcatccat tgcgccgaag ttgtaggacg cggcgctatt cagcaccagc gcgccgagcg
     2521 tggcgccgga gtagcccggc gtcagcgcgc cgccgctcgc gcgcgcatcg agcgtgaacg
     2581 cgagatccat gcgctcgttc atgtaggtga acgtcgagcc ggcgcaggcg ccgatgtccg
     2641 tgcgcggcgt gatcgcggcg cccgccgcca gcgtgaagtg gtccggcacg aagcggccca
     2701 cggtgagcgt ggccgagctg atatagcgca ccgaggtcgc cgtgccgtcg ccggcgtcga
     2761 cgctggcgaa ggtctggtcc tgcagggtca ggttgaacgc ccccacgtcg gcgtaggtcg
     2821 cggtggtggt ggtgatcacg ccggccgccg ccgaccaggc gccgaggccc agcgtgccgg
     2881 gggtggccgg gcacaccgcc cccgcgccgc actgcgcgag gatcgccacc ggctgcccgg
     2941 cgccggggct gtacagcgtg gtggttgccg gcgccccagc cccgttctgc gccgtggcgt
     3001 cgacgcggaa cggccggccg gcgttgtgga cggtgccgat accgggattc gatgtgttgt
     3061 tgagcgtgcg cgtcgtgccc gcggtcacgc ggtcctggtc gcgcaccagc acgctcgcga
     3121 actgcagcgg ccggttcgcg aaggcgtcgc tggagcagct cgtgaccgta ggcgcggcga
     3181 ccgggaactt gacgcggaac agcacattgc gccaggcatt ggcctcgaac tggcccgcgc
     3241 tcaagggatg ccgcccggca tcgccgccgg tgaaggtctg gtcggcgagc gccttgatga
     3301 gcggcaggcc cgcgcaggcg ccgccgctgg tattgtcgac cagctccacc gcgacggtgc
     3361 cggtaaagcc ggcggcgacc atgttggagg cgtcgcgcgc gacgatatcg accgcgatgt
     3421 cctgcccggc gatcctggtg aagatgcgtc cggtgaccgc gtcgccgccg ggctcgacca
     3481 cgttgaagct cgacaccgcg gcggcgacgt cgagcaccgc gaatgtcggc gcgccgttgc
     3541 ccgcgttggt ggtcgtcacc gtgccggtgc cgttgttgta gctgcccgcg accgccgagg
     3601 tcacgctcac gctcaccgtg cagctgctcg cggccggaat cgtgccgccg ttcagcgtga
     3661 gctgcgtgcc gcccgcaacg gcgctcgccg cgccgccgca actgttgacg aggcccggca
     3721 cggcggcatt gacgagattc gccggatagc tgtcggtgaa ggccaggccg gcgatggcga
     3781 aggtgttcag attggtgacc atgaccgaca gcgtgctcga ttggccggcg atgatcgacg
     3841 aagggctgaa gctcttcgcc accacgggcg gcgggaagaa ggtcaccgtc gcggtctgcg
//
LOCUS       DASWHU010000319         5298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_928367, whole genome shotgun sequence.
ACCESSION   DASWHU010000319 DASWHU010000000
VERSION     DASWHU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928367"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VGB88_11870"
     CDS             complement(<1..1147)
                     /locus_tag="VGB88_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9648190.1"
                     /translation="MTAALVEGAGRELGAIYAPEDGERLDARIAALTDSRLRARAAAS
                     DLVAALPGLLAALGWRAGAGPLLDLLPDAGHGFGLGELCRVLRRAGYQPTVRRLRRLA
                     AGETALPCLVVPPASPAVVALEARGPRLLIYDPAAAGMRLVAADAYQRVQVVEARPRG
                     AGEAPSEGWLRRRLARLRPVAWLALALVLAANLLSLGVPLFVMAVFDLAVGYATTGVL
                     PLLVAGIAGILAFELVLRWLRRELLIRAGAELSHALSTAVFGKLLSLPLDRVEGAGTR
                     SQIARMKDIDQMRGAVGGPLALAVLELPFVALALLVVGLLAGSVVWVPLAALLVSVAV
                     GLPLTAWVNGCQAAASRAVGRRQDLALEVVRDMRALRVASAADRWLDR"
     gene            complement(1144..2829)
                     /locus_tag="VGB88_11875"
     CDS             complement(1144..2829)
                     /locus_tag="VGB88_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648191.1"
                     /translation="MTTPAAPSGVAVVDRLTRDRRARLELIGVSLVINLLGLALPLVL
                     LQVYDRVIPYRAPETLVALALIVIAACVIETVLRLGRAALTARLGAGLEHRLGVAAVG
                     HLFRSDVARLEAAGCGTLFERLNAIPALRERMAGDVGLIASELAFVPLFLILLAHLGG
                     WLALAPIAVLALLALAAPIAAGTVQAAFVRHAENEDRRSAFLISAFNAVRGIKCIAAE
                     PPLEEQYRRLDRARGASAIAAERQSAAAGDLAATLGQAGIVATAAFGAAMVVDGALSA
                     GALAACILLAGRALQPAQRAVTLWPRAQAAAMARDRLAALFELPVAPVGGRIAGPIRG
                     QVSLRDVAVGVSDAGGPVLAGINLSVRPGEMVALVGPNGCGKTLLLSVIAGLARPSAG
                     TVLIDGRPLDDHDPGGLRRSIAYLAQHETLFQGSIMDNLTMFRPHAEQRALAAAAFIG
                     LADEVRAMPAGFDTAVGAGRAHGISRCLAQRIAIARALVDRPRLVIFDEANSELDAAA
                     DDKLKVALAALRGRCTVIIAGYRPSILRLADRIHDVGSGSVDPGGLTVRAAAP"
     gene            3284..3667
                     /locus_tag="VGB88_11880"
     CDS             3284..3667
                     /locus_tag="VGB88_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9648192.1"
                     /translation="MAPAPANPGIRMDAETVGDMVVVTLREARLDATAAVAFRQRIAE
                     LTDGGARTLVLDMSAVEVVDPSGLGALISVRNATRANAALALCGLRPSAAAAFRLTRL
                     DKLFRIFADPQEARAALTERPGGAG"
     gene            complement(3680..4669)
                     /locus_tag="VGB88_11885"
     CDS             complement(3680..4669)
                     /locus_tag="VGB88_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX9648193.1"
                     /translation="MSAQVLVGDIGATNVRLALAEPGRRPGAHVAVAADAHANLAQAV
                     EAGLAALAPRTRPRHAALALAGPVIGDGFAFTNRPAWAFSAAALCRTLGLERLTLVND
                     LAALALALGLAALGEADTVPLGGPARPADPTAPCAVLGSGTGFGVAALVRCDGEAVAV
                     AGEGGHAGLAARNQAEAKILRTLRDRGLRPTPDRLLSGPGLGLLYAAIAGTDADAAPA
                     AAEIARARHSDPIAARTLDCFARWLGGFAGDVVLILGARGGAFIGGGVVPALGPAFPV
                     ATFRAGFEDKGRFRDYMAAIPTRLVVSEAAVLAGALAAAGRGGSGIVTVTGGR"
     gene            complement(4666..>5298)
                     /locus_tag="VGB88_11890"
     CDS             complement(4666..>5298)
                     /locus_tag="VGB88_11890"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648194.1"
                     /translation="LGGAPNPVAAARWYRLAAEQGHLDSQTNLGRLYLSGDGLPRDPA
                     QASDWFRRTADRDFAPAQYYLAELYRTGDGVTIDPETAVALHQAAAEQGLPESAFQLS
                     RMYRNGEGVARDPREARKWLVRAAVAGMPEAAHALAGLYLSGEGEEPDLVRAYLWSAL
                     AAQAGIKDAGEMRESIAERLTPEQRDEARRLAAESGKALESLDPDKATAQ"
BASE COUNT          783 a         1985 c         1938 g          592 t
ORIGIN      
        1 agcggtccag ccagcgatcc gcggcgctcg ccaccctgag cgcgcgcatg tcgcggacca
       61 cctcgagggc aaggtcctgg cgtcgtccga cggcgcgcga ggcggcggcc tggcagccgt
      121 tgacccaggc ggtcagcggc aatccgaccg cgaccgagac gagcagcgcc gccagcggca
      181 cccagacgac gctgcccgcc agcagaccga cgacgagcag cgccagcgcc acgaacggca
      241 gctcgaggac ggcgagcgcg agcgggccgc cgaccgcgcc gcgcatctgg tcgatgtcct
      301 tcatgcgcgc gatctgcgac ctggtgccgg cgccctcgac ccggtcgagc ggcaacgaca
      361 ggagcttgcc gaacaccgcg gtcgagagcg cgtgcgacag ctcggcgccg gcgcggatca
      421 gcagctcgcg cctgagccag cgcagcacca gctcgaaggc gaggatgccg gcgatgccgg
      481 ccaccagcag tggcaggacg ccggtcgtcg catagccgac cgccaggtcg aacaccgcca
      541 tcacgaacaa cggcacgccg agcgacagca ggttggcggc gagcaccagc gcgagcgcca
      601 gccaggcgac cgggcgcaag cgggcgagcc ggcgtctgag ccacccctcg gacggtgcct
      661 cgccggcgcc gcgcgggcgc gcctcgacaa cctgcacccg ctgataggcg tcggcggcga
      721 ccagccgcat gcccgccgcc gccggatcgt agatcagaag ccgcggccca cgcgcctcga
      781 gggcgacgac cgccgggctc gccggcggta ccaccaggca cggcaatgcc gtctcgccgg
      841 cggcgaggcg gcgcaggcgc cgcaccgtcg gctggtagcc ggcgcgacgc agcacgcggc
      901 acagctcgcc aagaccgaag ccgtgcccgg catcgggcag caggtcgagc agcggcccgg
      961 ccccggcgcg ccagccaagg gcggcaagca ggcccggcaa cgcggcaacc aggtccgacg
     1021 ccgccgcgcg cgcgcgcaac cgggaatcgg tcagcgccgc aatgcgcgca tcgagtcgct
     1081 cgccgtcctc gggcgcgtag atcgcgccga gctcccgccc cgccccctcg accagcgccg
     1141 cggtcatggc gcggccgccc gcactgtcag gccgcccggg tccacgctcc ccgagccgac
     1201 atcgtgtatc cgatcggcga ggcgcaggat cgacggccgg tagccggcga tgatcaccgt
     1261 gcaacggcca cgcaacgccg ccagcgcgac cttcagcttg tcgtcggcgg cagcgtcgag
     1321 ctcgctattg gcttcatcga agatcaccag ccgcggccgg tcgaccaggg cgcgggcgat
     1381 ggcgatgcgc tgggcgaggc agcgtgagat gccgtgcgcc ctacccgccc cgaccgcggt
     1441 atcgaagccc gcgggcatcg cccgcacctc gtcggcgagg ccgatgaacg ccgcggcggc
     1501 aagcgcccgc tgctcggcgt gcgggcggaa catggtcagg ttgtccatga tcgagccctg
     1561 gaacagcgtc tcgtgctggg cgaggtaagc gatggaccgg cgcagcccgc ccgggtcgtg
     1621 gtcgtcgagc gggcggccat cgatcagcac ggtgccggcg ctcggccgcg ccaggcccgc
     1681 gatcaccgac agcagcaaag tcttgccgca gccgttgggg ccgaccagcg cgaccatctc
     1741 tcccggccgg acggacaggt tgatgccggc aagcaccggg ccgccggcgt cgctgacacc
     1801 gaccgcgacg tcacgcaagc tcacctggcc gcggatcggg ccggcgatgc gcccgccgac
     1861 cggcgccacc ggcagctcga acagcgcggc gaggcggtcg cgggccatgg cggcggcctg
     1921 cgcgcgcggc cacagcgtga ccgccctctg cgccggctga agggcgcggc cggcgagcag
     1981 gatgcacgcg gcgagcgcgc ccgcgctgag cgcgccgtcg acgaccatgg cggcgccgaa
     2041 ggcggcggtg gcgacgatgc cggcctgacc cagcgtcgcc gccaggtcgc ccgcggcagc
     2101 cgactgccgc tcggcggcga tcgcgctcgc gccgcgcgcc cggtcgaggc gccggtattg
     2161 ctcctcgagc gggggctcgg cggcgatgca cttgatgccg cgcacggcat tgaacgcgct
     2221 gatcaggaac gccgagcgcc gatcctcgtt ctcggcgtgc cggacgaacg ccgcctggac
     2281 cgtgccggcc gcgatcggag cggcgagcgc cagcagcgcc agcacggcaa tcggcgccag
     2341 cgccagccag ccgcccagat gggccagcag gatgaggaac aacggcacga acgcgagttc
     2401 tgaggcgatc aggccgacat cgcccgccat gcgctcgcgc agcgccggga tggcgttgag
     2461 gcgctcgaac agggtgccgc agcccgcggc ctcgaggcgc gcgacgtcgc tccggaacag
     2521 gtggccgacg gcggcgacgc cgagacggtg ctcgagcccc gccccgagac gtgcggtgag
     2581 cgccgcgcgg ccaagtcgca agacagtctc aataacgcag gccgcgatca cgatcagcgc
     2641 cagcgccacc agggtctcgg gtgcccggta ggggatcacc cggtcgtaga cctgcagcaa
     2701 caccagcggc agcgccaggc cgagcagatt gatcaccagg gacacgccga tcagctccag
     2761 gcgcgcgcgc cggtcgcgcg tcaggcgatc gacgaccgcc acgcccgagg gcgctgccgg
     2821 cgtggtcacc gcgatcgacc cgatcggtcg acgaccgggg ccggcggggc cgccacccgg
     2881 cgtcccgccg cctccccggc catgccgcga tcggccttcg ccaaggccgc gattgggctc
     2941 atggtcccgc tgcacaatac ccgcatagat tgtctcgaat tatagaccga atttgacgtt
     3001 atacaatctt tggctgaagt gtgaaaagac accgttgtca gcatggcaaa agtttccgca
     3061 cgattcctgg agattgatcg tacggattga cggtttataa tcgtttaagt agatcaccgt
     3121 cttgcccggc cgtgtccggc cggcaggaga ttggctgtgc atcgcgcgac atggcaaaga
     3181 aatttcacgt attggttgaa gaaacctcgg ccccgatctc gccccgtccg gcgtccgtct
     3241 gcggcgcctc cggttcgcgc ccgcaccgcc catgagcggg ccggtggcac cggcgccggc
     3301 aaacccgggc atcaggatgg atgccgagac cgtcggcgac atggtcgtgg tcacgctgcg
     3361 cgaggcgcgg ctggacgcga ccgcggcggt cgcgttcagg caacgtatcg ccgagctgac
     3421 ggacggcggc gcgcggacgc tggtgctcga catgtcggcg gtggaggtgg tcgacccgag
     3481 cgggctcggc gcgctgatat cggtccgcaa cgcgacgcgc gcgaacgctg cgctcgccct
     3541 gtgcggattg cgcccgtcgg cggccgcggc cttcaggctg acccgcctgg acaagctgtt
     3601 ccgcatcttc gccgacccgc aggaggcgcg cgcggcgctc accgaaaggc cgggtggggc
     3661 cggttgacgg cggctggcct cagcgcccgc cggtgaccgt gacgatgccg gacccgccgc
     3721 gcccggccgc cgccagcgcc cccgccagca ccgccgcttc gctcaccacc aggcgggtcg
     3781 ggatcgccgc catgtagtcg cgaaaccggc ccttgtcctc gaagccggcg cgaaacgtcg
     3841 ccaccggaaa cgccgggcca agcgccggca ccacgccgcc gccgatgaat gcgccgccgc
     3901 gcgcccccag gatcagcacg acgtcgccgg cgaagccgcc gagccagcgg gcgaagcagt
     3961 cgagggtgcg ggccgcgatc gggtcgctgt ggcgcgcgcg cgcgatctcc gccgccgccg
     4021 gcgccgcgtc tgcgtcggtg cccgcgatcg cggcatagag caagccaagg ccgggccccg
     4081 acagcagccg gtcgggtgtc gggcgcaggc cgcggtcgcg cagggtgcgc agaatcttcg
     4141 cctcggcctg gttgcgcgcg gcgagcccgg catgcccgcc ctcgccggcc acggccacgg
     4201 cctccccgtc gcagcggacc agagcggcga cgccgaagcc ggtgccggag ccgagcaccg
     4261 cgcacggggc ggtcgggtcc gcggggcgcg cggggccgcc gagcggcacc gtgtccgcct
     4321 cgccgagcgc ggccaggccg agcgcgagcg cgagcgcggc aaggtcgttg accagggtca
     4381 gccgctcgag gcccagggtc cggcacagcg cggccgcgga gaacgcccac gccgggcgat
     4441 tggtgaacgc gaagccgtcg ccgatgaccg ggccggcgag cgccaaggcg gcgtggcgcg
     4501 ggcgggtgcg cggcgcgagc gcggcgagac ccgcctcgac cgcctgggca aggttcgcat
     4561 gggcatccgc ggcaacggcc acatgcgcgc cggggcgtcg tccgggctcg gccagcgcca
     4621 ggcggacatt ggtggccccg atatcgccga ccagcacctg cgcgctcact gtgccgtggc
     4681 cttgtccgga tcgagcgact ccagcgcctt gccactctca gccgctagcc ggcgggcctc
     4741 gtcgcgctgc tcgggcgtga gccgctcggc gatcgactcg cgcatctcgc cggcgtcctt
     4801 gatgccggcc tgcgccgcca gcgcggacca cagataggcc cgcaccaggt cgggttcctc
     4861 gccctcgccg ctcaggtaga ggccggccag cgcgtgcgcc gcctcgggca ttcccgcgac
     4921 cgccgcccgc accagccact tgcgcgcctc gcgcggatcg cgcgcaacgc cctcgccgtt
     4981 gcggtacatg cgcgacagct gaaacgcgga ctcgggcaag ccctgctcgg ccgccgcctg
     5041 gtgcagcgcc accgccgtct ccgggtcgat ggtcaccccg tcgccggtgc ggtacagctc
     5101 ggcgaggtaa tactgggcgg gcgcgaagtc ccggtcggcg gtgcgccgga accagtcgga
     5161 cgcctgcgcc gggtcgcgcg gcagcccgtc gccgctcagg tacagccgcc cgagattggt
     5221 ctgggagtcg agatggccct gctcggccgc cagccggtac cagcgcgcgg cggcgaccgg
     5281 gttgggcgcg ccgccgag
//
LOCUS       DASWHU010000320        15477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_929062, whole genome shotgun sequence.
ACCESSION   DASWHU010000320 DASWHU010000000
VERSION     DASWHU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15477
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929062"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGB88_11895"
     CDS             <1..882
                     /locus_tag="VGB88_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX9648195.1"
                     /translation="SCRYLDSLPVAGSAAGHAIRDPALEQEILEMTRNFGIGAQFGGK
                     YFCHDVRVVRLPRHGASLPIGIGVSCAADRQALAKITADGVFLEQLETNPAKYLPEVE
                     PRQLAGEVVEIDLARPMDEIRRELSRHPIRTRVKVSGPMIVARDAAHARLREALEAGE
                     DLPRYFKDHCVYYAGPAKTPAGYATGSFGPTTAGRMDDYVDQFQAHGGSLVMLAKGNR
                     SRAVVEACKTHGGFYLGSIGGPGARLAQDCIRKVDCIAYPELGMEAIWRIEVEDFPAF
                     IVIDDKGNDFFTEINRR"
     gene            893..1255
                     /locus_tag="VGB88_11900"
     CDS             893..1255
                     /locus_tag="VGB88_11900"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX9648196.1"
                     /translation="MPERPEIIDHVSDLLAPLGPVEPRFMFGGWGLYLGGVMFALIAD
                     GELYLRTDSRTAPAYEAQGLPRFKPWDDKPVTMPYHLAPEAAFETAEAMVALAQDALA
                     AARQAKRGKRSRTGKARA"
     gene            1252..2658
                     /gene="fumC"
                     /locus_tag="VGB88_11905"
     CDS             1252..2658
                     /gene="fumC"
                     /locus_tag="VGB88_11905"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9648197.1"
                     /translation="MSGTAGTRTETDSLGPVEVPAERYWGAQTERARRNFPIGEERMP
                     LELVHALALVKRAAAEVNRDLGRLDPALAEAIAAAAAEVADGALDGEFPLPVWQSGSG
                     TQTNMNVNEVIAGRANERLGAGRGGKRPVHPNDHVNLSQSTNDSFPTAMHVAAALSFR
                     DRLRPALAGLSAALADKARAFADLVKIGRTHLQDAVPITLGQEFAGYASQVGAAIERL
                     DQVLARLCPLAQGGTAVGTGLNAPPDFGARVAARLGALTGIPFTAAADRVSAIAAHDA
                     LVDASAALATLATALTKIANDIRLMGSGPRSGLGELVLPANEPGSSIMPGKVNPTQAE
                     ALTMVCAQVIGNHVAVTVAGAGGHFELNACKPVIIDNLLRSARLLADAAASFTERCVR
                     GLEADQARLTELVERSLMLVTALSPHIGYDRAAAIATKAHREGLTLRAAALALGSVSA
                     ADFDRWVRPETMVGPGEA"
     gene            2655..2885
                     /locus_tag="VGB88_11910"
     CDS             2655..2885
                     /locus_tag="VGB88_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX9648198.1"
                     /translation="MTGLSKVSVTIGGHRTSLSLEPAFWRAFAELCRAEGRSRAAVLA
                     EIDGARRSNLSSAVRLWVLERLSRGARLSAAG"
     gene            complement(2869..6960)
                     /locus_tag="VGB88_11915"
     CDS             complement(2869..6960)
                     /locus_tag="VGB88_11915"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9648199.1"
                     /translation="MRSLLYGFLAVLLVVIGALLIGPSFVDWNRFKPEIVDQLAKLTG
                     RDIAIGGPIAFAMLPTPTLSAERVRVANLDGAADPDLISLDSVGVRVALVPLVAGKVR
                     IDSVILAAPRLQLERLADGRVTWRLGPPEAADEAGGSAPAAPPGLSLFGLPLEISVER
                     VTVERGRLVYRDSAAGIEETVDDIDVVLSAGSLSGPFTMRGRLVARGLPVDADVALGD
                     LRPAQRVAMRARLSSGGVSVGFTGGGTPTAERPAVDGKATVTSASLAELAGLVAAATG
                     RTVTARMPRRQRLAIEAQVAATENDLSANDVEIRFGDTVAKGKLTTKFAGGGQFELAL
                     DANRIDIDALIADLAGQPATTGQDAGPQDDEGGAAPVAVWAPPRGLNGSLDLTVDAVL
                     YRDQIVRQTVVNLGVAGGMVNIQRLSALLPGGSDVTLFGSIEPRPEGPHFSGQLDAAS
                     DNLRALLAWFNVDISRIPADRLRKFSLSAAIDGDPKNGQLTGIDVGLDTTRMIGGIAY
                     AMRARPAFGVNLRIDRVNVDAYLQAPAAGVPLPATRPAPPGPAVPGAAPEQPEPEPAR
                     RPLAVLADFDADFVIQADSLTWREVPVSGIKLDGLLQGGALTFRDARVADLAGARAAL
                     AGRIEQLGGTPAVDVALKFDTADLSAMSRMVPALRGLPPAFAGALSVDTKLKGDFDSL
                     AIDGALEALASRVAVDGTIRDLAGTPRFELKAGLSNTNLAGLLDAVGALARADAPAVL
                     AAPVTVSTALAGTLAGFDVDAGGSLADARLGLKGRIEGLPAPARFDLVIEAEHPSLAR
                     LLGRSAALDAGTPPPALAGPVTVEGSAAGTVAGFDLDARLKAAGAEASAQGKIEDIAD
                     APRYTLTAAASHPDMQALLDGLGVDVETDAYAGPATVSGRLSGGPSAVSVPNLSANLG
                     GSSVVGSVSVALDGARPRLTADLITGPLVVEHFLGVAAAVAPGIAGGLPPAPDTGSGA
                     ERWSRAPLELSALRAFDASVKLSTDALGYDGYRFEAAEVDLGLDNGTLQVRRLVGRLF
                     GGDLSCEGQLVSGAVPSARLAIELAGVDIRAALGRLAGIENITGRIGFEGSFTTRGSS
                     AQQMMLALNGRGRFRDATGGIIEGLDLAALSRRLDEIEGPQGITGLLAAGFEAGRTRY
                     SQLRGTFSITDGRIATEDTYLAADGGTGSLTGGADLPAWQVDATAMFTLADHPEVPPI
                     GVRLSGPLEAPQKQIEAARLDQFLIEKSARELLQEPPPGNEEASEAPAPEAEAPLAEV
                     DVPAAEAPVPEPAATAQPMPEPVAKPTPKPAPPVEPAPEPVAEPAPTPPAEPEPTPQA
                     APAPAPAAGTPPPPPPAAAPAQPAPRPQEKFEDVLEDLLSPPR"
     gene            complement(7554..8960)
                     /locus_tag="VGB88_11920"
     CDS             complement(7554..8960)
                     /locus_tag="VGB88_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9648200.1"
                     /translation="MAVAPAQARSRLDPRSPLIAELRALFAERLSVSAAVRELHGRDE
                     SYHTPYSPDAVVFARTTEEVSDAVTLCARYCAPVIAYGTGTSLEGQVAALEGGISIDV
                     SQMTEVVEVNPEDLDCTVQAGVTRKQLNEYLRDTGLFFPIDPGADASLGGMAATRASG
                     TNAVRYGTMRENVLALTVVLPDGRVIRTARRARKSAAGYDLTRLFVGSEGTLGVITEV
                     TLRLAGIPEAISAATCSFPSIGDAVDAVIVTIQSGIPVARIELIDKVSMEAINAFSKT
                     DYPVAYTLFYEFHGSQASVREQAEHAQEFAADHGGGDFRWTADHEQRNKLWQARHDAY
                     YAMRALCPGTKGMVTDVCVPISRLAECVVETEKDNATAPFKCPIVGHVGDGNFHVSYM
                     IDPDDQAQISFGARLHDRLVARALAMEGTCTGEHGVGYGKIDFLTEELGEALSVMRAI
                     KRAIDPDNIMNPGKIVRL"
     gene            9059..9562
                     /locus_tag="VGB88_11925"
     CDS             9059..9562
                     /locus_tag="VGB88_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9648201.1"
                     /translation="MTSPAHRRSQPLVLHREPVREAWLDYNGHMNVAYYLLAFDHASD
                     AFAEHIGIGQAYTRTSGRSIFILECHVAYLREVVGGDPLRFTTHLLGFDDKRIHFAHA
                     MHHDVRSYHVATGELMLVHVDLAQRKATAMPAPTLAALARIASDQQGLERPVGLSRVM
                     GLGAGRP"
     gene            9668..9907
                     /locus_tag="VGB88_11930"
     CDS             9668..9907
                     /locus_tag="VGB88_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648202.1"
                     /translation="MSIPPVNFPLPSPIASALKAIRDANETVAEVAQDVAEHGAEGFA
                     ENAVALTTAEISAKAAIAVLRTAAELEQETLDILA"
     gene            10001..10396
                     /locus_tag="VGB88_11935"
     CDS             10001..10396
                     /locus_tag="VGB88_11935"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX9648203.1"
                     /translation="MAGSPSPARKPTVVGHDDRWLRLLYMVIFAIIGYTVFWVVLFLA
                     AVQFLLTWINGEPNHNLRDFAGKLDAYLQAVLDFIGYATDVVPFPLSPLETSLASAAP
                     AAARRRSRSAGARAKGGAAAKTEPGAEDG"
     gene            10550..12247
                     /locus_tag="VGB88_11940"
     CDS             10550..12247
                     /locus_tag="VGB88_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9648204.1"
                     /translation="MLALVIGAAAGYGAIAFRLGIDAIQLLFYGSATQQVLSMARHLE
                     WWHRLLAPALGGLGVGLFIHYALPERRPHGVADVIEACALRGGRMRLRDGLAAGFASA
                     ASIGVGASVGREGPVVHLAAAMAGALGGRLGLDRGQLRTLLGCAVASGIAASFNAPIA
                     GVFFALEVVVGHYALGAFAPVVIAAVIGTIITRAQFGDFPAFVVPGAEIASYYEMPAF
                     LLLGAVCALVALLFMSGTMFTAKVVARVPVAGYLKPAAGGLLTGAIAAAFPEVIGVGY
                     ETTDLALTGAFTLDVLLLLVVAKTTASVICLGSGFAGGVFSPSLCIGALTGAAFGIIA
                     TAVVPEAQASINAYSLVGMGAVAGAVLGAPISTILIIFELTGDYAVTIAVMLSVVTAS
                     LITRRVADGSFFTLQLRARGLDPASAEARAPSRAITVRSLMSDSFRTVDADTPVGEIR
                     ELLRSAPDYDLYVTDDAGRLTGVVPFDRLRALLFDPSPDLDARASSLAVEALDVLVPG
                     DTLDLALDMLETGQARHLPVVEDRLARRLIGVVFRTDALHAYNRALLRERALEHGSG"
     gene            12326..14617
                     /locus_tag="VGB88_11945"
     CDS             12326..14617
                     /locus_tag="VGB88_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9648205.1"
                     /translation="MVDAFDPFDLTQIAPAARPVSAPGAERYLEALNEAQRRAVECTE
                     GPVLVLAGAGTGKTRVLTTRLAHILESRLAHPSETLAVTFTNKAAREMRERVERLLGR
                     SVEGWHLGTFHAVAARILRRHAECVGLKSAFTIIDADDQVRLLKQLLAAENIDEKRWP
                     ARTLAAIIGRWKDRGLTPDKVSRTDIGEFAAGRALDLYRAYQERLATLNAADFGDLLL
                     HNLTLFARDARVLADYQDRFRYVLVDEYQDTNVAQYLWLRLLAQKHRNLCCVGDEDQS
                     IYAWRGAEIGNILRFEHDFPGATVIRLERNYRSTPHILGAASGLIAHNESRLGKTLWA
                     EGEAGETVRVVGVWDGEEEARLVGDQIESLHRRGQPLRESAILVRASFQTRAFEERFI
                     QIGLPYRVIGGLRFYERLEIRDALAYLRVIGQPADDLAFERIVNRPKRGIGDASLQTL
                     NLHARTRGIALFDAARELVESDELRGRARAAMRALLDSLDRWRALAAGGTELPALAET
                     VLDESGYTGMWQQDRSADAPGRLENLKELVIALEEFDGLGGFLEHVALVMDNAAGAGE
                     DGVNLMTLHGAKGLEFDTVFLPGWEEELFPNRRALEDGGVRALEEERRLAYVGLTRAR
                     KRAMVLYAANRHAFGQWLSAAPSRFLAELPAAHVARDSVPAAYYGGLSEAEVADPSGF
                     DRPAIGARRSNWARYAARYGAEPALPVETRSHAPGTFAPGSRVFHQKFGYGEVRAIDG
                     NKLEIAFDKAGTKKVMDSFVTPA"
     gene            complement(14604..>15477)
                     /locus_tag="VGB88_11950"
     CDS             complement(14604..>15477)
                     /locus_tag="VGB88_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9648206.1"
                     /translation="ARCVMILLERLLDGLEVAVQPFSVSRLAAGESAHGGRRDEAVVH
                     YLLDGAGGLSIEGLPAIRLERRSAVIVPAGRSHRFAADGEAPGEGGVVLACGAIGATF
                     HRGHGLFDYLPEPIVVRGAADRAFADAFEALLAELAHPQPGTDVLVRALMQQCLVHVL
                     RRYCESGECRVPWLIALEDERIGCVVRAMLDDPGRPFTVERLAEVAGMSRSTFAEHFR
                     HAFGRGPIDFLKEVRLRRAAALLVTSERPVKAVGAAVGYDSRSHFSRAFFALYGCTPT
                     AWRANTRAGALTPG"
BASE COUNT         2092 a         5699 c         5589 g         2097 t
ORIGIN      
        1 agctgccgct atctcgactc gttgccggtc gcgggcagcg ccgcggggca cgcgatccgc
       61 gaccccgcgc tcgagcaaga gatcctcgag atgacccgca acttcggcat cggcgcccag
      121 ttcggcggca agtatttctg ccacgacgtg cgcgtcgtgc gcctgccgcg ccacggcgcc
      181 agcctgccga tcggcatcgg cgtctcctgc gccgccgacc gccaggcgct ggccaagatc
      241 accgcggacg gggtgttcct cgagcagctc gagaccaatc cggccaagta cctgcccgag
      301 gtcgagccgc ggcagctcgc cggcgaggtg gtcgagatcg acctcgcccg gccgatggac
      361 gagatccggc gcgagctcag ccggcacccg atccgcaccc gcgtcaaggt ctccggaccg
      421 atgatcgtcg cccgcgacgc cgcccatgcg cgcctgcgcg aggcgctcga ggcgggcgag
      481 gacctgccgc ggtacttcaa ggaccactgc gtctactatg ccgggccggc caagaccccg
      541 gcgggctacg ccaccggctc gttcggcccg accaccgccg ggcgcatgga cgactatgtc
      601 gaccagttcc aggcccacgg cggctcgcta gtgatgctgg ccaagggcaa ccgctcgcgc
      661 gccgtggtcg aggcgtgcaa gacgcatggc ggcttttacc tcggctcgat cggcggcccc
      721 ggcgcacgcc tcgcccagga ctgcatcagg aaggtcgact gcatcgccta ccccgagctc
      781 ggcatggagg cgatctggcg gatcgaggtc gaggactttc cggcgttcat cgtcatcgac
      841 gacaagggca acgacttctt caccgagatc aaccgccgct gagagtgcgc ccatgcccga
      901 gcgcccggag atcatcgacc acgtaagcga cctgctcgcc ccgctcgggc cggtcgagcc
      961 gcggttcatg ttcggcggct ggggcctcta tctgggcggg gtgatgttcg cgctgatcgc
     1021 cgacggcgag ctctacctgc gcaccgattc gcgcaccgcc cctgcctacg aggcccaggg
     1081 cctgccccgg ttcaagccgt gggacgacaa gcccgtgacc atgccctatc acctcgcgcc
     1141 cgaggccgcg ttcgagacgg ctgaggcaat ggttgccctg gcacaggacg cgctggcggc
     1201 ggcgcggcag gcgaagcgcg gcaagcggtc gcgcaccggc aaggcgcgcg cgtgagcggc
     1261 acggcaggca cgcgcaccga gaccgacagc ctgggtccgg tcgaggtccc ggccgagcgc
     1321 tactggggcg cacagaccga gcgcgcgcgc cgcaacttcc cgatcggcga ggagcgcatg
     1381 ccgctcgagc tggtccacgc cctggcactg gtcaagcgcg ccgccgccga ggtcaaccgc
     1441 gacctcggcc gcctcgaccc cgccctcgcc gaggcgatcg cggcggccgc ggcggaggtc
     1501 gccgacggcg ccctcgacgg cgagttcccg ctgccggtgt ggcagtcggg ctcgggcacc
     1561 cagaccaaca tgaacgtcaa cgaggtgatc gcgggccgcg ccaacgagcg cctcggcgcc
     1621 ggccgcggcg gcaagcgccc ggtccacccc aacgaccacg tcaacctgtc gcagtcgacc
     1681 aacgacagct tcccgaccgc gatgcacgtc gccgccgcgc tctcctttcg cgaccgcctg
     1741 cggccggcgc tcgccgggct gagcgcggcg ctcgccgaca aggcgcgcgc gttcgccgac
     1801 ctggtcaaga tcgggcgcac ccatctccag gacgcagtgc cgatcaccct cggccaggag
     1861 ttcgcgggct acgcctccca ggtcggggcg gcgatcgagc gcctcgatca agtgctggcg
     1921 cgattgtgcc cgctggcgca gggcggcacc gcggtcggca ccggcctcaa cgccccgccg
     1981 gacttcggcg cccgcgtcgc ggcccggctc ggcgcgctca ccggcatccc gttcaccgcc
     2041 gccgccgacc gggtctccgc gatcgccgcc cacgacgcgc tggtcgatgc ctcggccgcg
     2101 ctcgccaccc tcgccacggc gctgaccaag atcgccaacg atatccggct gatgggctcc
     2161 gggccgcgct cggggctcgg cgagctggtc ctgccggcca acgagcccgg ctcctcgatc
     2221 atgcccggca aggtcaaccc gacccaggcc gaggcgctga ccatggtctg cgcgcaagtg
     2281 atcggcaacc acgtcgcggt gacggtcgcc ggcgcgggcg gccatttcga gctcaacgcg
     2341 tgcaagccgg tcatcatcga caacctgctg cgctcggccc ggctgctcgc cgacgccgcg
     2401 gcgagcttca ccgagcgctg cgtgcgcggg ctcgaggccg accaggcgcg gctcaccgag
     2461 ctggtcgagc gctcgctgat gctggtcacc gcgctcagcc cgcatatcgg ctacgaccgc
     2521 gccgccgcga tcgcgaccaa ggcgcaccgc gagggcctca ctttgcgcgc ggcggcgctt
     2581 gcgctgggca gcgtctcggc cgcggacttc gaccgctggg tgcgacccga gacgatggtc
     2641 ggcccgggcg aggcatgacc ggcctcagca aggtctcggt gaccatcggc ggccaccgca
     2701 ccagcctgtc gctcgagccc gcgttctggc gcgccttcgc cgagctgtgc cgcgccgagg
     2761 gccgcagccg cgccgcggtg ctggcggaga tcgacggcgc gcggcgcagc aacctgtcga
     2821 gcgcggtgcg cctgtgggtg ctcgagcgcc tcagccgcgg cgcgcggctc agcgcggcgg
     2881 gctgagcagg tcctcgagca cgtcctcgaa cttctcctgc gggcgcggcg cgggctgcgc
     2941 cggggccgcc gcgggcggcg gcggcggcgg cgtccctgcc gcgggcgccg gcgcgggcgc
     3001 ggcctggggg gtcggctccg gctccgcggg cggcgtcggc gcgggctcgg cgaccggctc
     3061 gggcgccggc tcgaccggcg gcgccggctt gggcgtcggc ttggcgaccg gctcgggcat
     3121 cggctgcgcc gtggcggcgg gctccgggac cggggcttcg gccgccggaa cgtcgacttc
     3181 ggcgagtggt gcctcggcct cgggcgcggg cgcttcgctc gcctcctcgt tgccgggcgg
     3241 cggctcctgg agcagctcgc gggcgctctt ctcgatcagg aactggtcga gccgcgccgc
     3301 ctcgatctgc ttctgcggcg cctccagcgg gcccgacagg cggaccccga tcggcggcac
     3361 ctcgggatgg tcggcgaggg tgaacatcgc cgtcgcgtcg acctgccacg cgggcaggtc
     3421 ggcgccgccg gtgaggctgc cggtgccgcc gtcggcggcc agatatgtgt cctcggtggc
     3481 gatccgcccg tcggtgatcg agaacgtgcc gcgaagctgg ctgtagcgcg tgcggccggc
     3541 ctcgaatccg gccgcgagca ggccggtgat gccctgcggc ccttcgatct cgtcgagccg
     3601 gcgcgacagg gcggcgaggt cgaggccttc gatgatgccg ccggtcgcgt cgcggaaccg
     3661 gccgcgcccg ttcagcgcca gcatcatctg ctgggcgctg ctgccgcggg tggtgaagct
     3721 gccctcgaaa ccgatccgcc cggtgatgtt ctcgatcccg gcgagccggc cgagcgccgc
     3781 gcggatgtcg accccggcga gctcgatcgc caggcgcgcg ctcggcacgg cgccggagac
     3841 cagctggccc tcgcacgaca ggtcgccgcc gaacagccgc ccgaccaggc ggcggacctg
     3901 gagcgtgccg ttgtcgaggc cgaggtcgac ctcggctgcc tcgaaacggt agccgtcata
     3961 gccgagggcg tcggtcgaca gcttgaccga ggcgtcgaac gcgcgcagcg ccgacagctc
     4021 gagcggcgcg cgcgaccagc gctccgctcc ggagccggtg tccggcgccg gcgggagccc
     4081 gccggcgatg ccgggcgcga cggcggcggc gacgccgaga aagtgctcga ccaccagcgg
     4141 cccggtgatc aggtccgcgg tcagccgcgg gcgcgcgccg tcgagggcga cggagacgct
     4201 gccgaccacc gacgagccgc ccagattggc gctcaggttc ggcaccgaga ccgcggacgg
     4261 cccgcccgac aggcgcccgg agacggtcgc gggcccggca taggcgtcgg tctcgacgtc
     4321 gacgccaagg ccgtcgagca acgcctgcat gtcgggatgc gaggcggcgg cagtcagcgt
     4381 gtagcgcggc gcgtccgcaa tgtcctcgat cttcccctgc gcggaagcct cggcgccggc
     4441 ggccttgagc cgggcgtcga ggtcgaagcc agcaacggtg ccggccgcgc tgccctcgac
     4501 cgtcaccggc ccggccaagg ccggcggcgg cgtgccggca tcgagcgccg cgctccgccc
     4561 cagcagccgg gcgaggctcg gatgctcggc ctcgatcacc aggtcgaacc gcgccggcgc
     4621 cggcaggccc tcgatgcggc ccttgaggcc gagccgggca tcggcgagcg agccgccggc
     4681 gtcgacgtcg aagccggcga gcgtgcccgc gagcgcggtc gacaccgtca ccggcgcggc
     4741 cagcaccgcc ggcgcgtccg cccgcgccag cgcaccgacc gcgtcgagca gcccagcgag
     4801 gttggtgttg gacaggcccg ccttgagctc gaatctcggc gtgccggcaa ggtcgcggat
     4861 cgtgccgtcg accgccaccc ggctcgccag cgcctcgagc gcgccgtcga tggcgagcga
     4921 atcgaagtcg cccttgagct tggtatccac gctcagcgcg ccggcgaagg cgggcggcag
     4981 gccgcgcagc gccggcacca tgcgcgacat cgccgacagg tcggcggtgt cgaacttgag
     5041 tgccacgtcg acggccggcg tcccgcccag ctgctcgatc cggccggcga gcgccgcccg
     5101 cgccccggcc aggtcggcga cccgggcgtc gcggaaggtg agcgcgccgc cctgcaacag
     5161 cccgtcgagc ttgatgccgc tgaccgggac ctcgcgccag gtgagcgaat ccgcctggat
     5221 gacgaaatcg gcgtcgaagt cggcgagcac cgcgagcggc cggcgcgcgg gctcgggctc
     5281 gggctgttcg ggggcggccc cgggcaccgc cggtcccggc ggcgccgggc gcgtcgccgg
     5341 caacggcacg ccggccgccg gcgcctgcag gtaggcgtcg acgttcaccc ggtcgatgcg
     5401 caggttgacc ccgaaggccg ggcgcgcgcg catcgcgtag gcgatgccgc cgatcatgcg
     5461 cgtggtgtcc aggccgacat cgatgccggt gagctggccg ttcttcgggt cgccgtcgat
     5521 cgccgccgac aggctgaact tgcgcagccg gtcggccggg atgcgcgaga tgtcgacgtt
     5581 gaaccaggcg agcagggcgc gcaggttgtc cgaggcggcg tcgagctggc cgctgaaatg
     5641 cggcccctcc ggccggggct cgatcgagcc gaacagggtg acgtcggagc cgccgggaag
     5701 cagcgccgac agccgctgga tgttgaccat gccgccggcg acgccgaggt tgaccaccgt
     5761 ctggcggacg atctggtcgc gatagagcac ggcgtcgacg gtgaggtcga ggctgccgtt
     5821 gagaccccgc ggcggcgccc atacggccac cggcgccgcg ccgccctcgt catcctgcgg
     5881 cccggcgtcc tggccagtcg tcgccggctg gccggcgagg tcggcgatca gcgcatcgat
     5941 gtcgatccgg ttggcgtcca gcgccagctc gaactggccg ccgccggcga acttggtcgt
     6001 cagcttgccc ttggcgacgg tgtcgccgaa acgaatctcg acgtcgttgg ccgacaaatc
     6061 attctccgtc gccgccacct gggcttcgat cgccagtcgc tggcggcgcg gcatgcgcgc
     6121 cgtcaccgtt cgcccggtcg ccgccgcgac caggcccgcc agttcggcga ggctggcgct
     6181 ggtgacggtc gccttgccgt cgacggcggg ccgctcggcg gtcggcgtgc cgccgccggt
     6241 gaagccgacc gacacgccgc ccgaggacag ccgcgcgcgc atcgccaccc gctgcgccgg
     6301 gcgcaggtcg ccgagcgcga cgtcggcgtc gaccggcagg ccgcgggcga ccaggcgccc
     6361 gcgcatcgtg aacgggccgg acagcgatcc ggccgatagc acgacgtcga tgtcgtccac
     6421 ggtctcttcg atgccggcag ccgaatcgcg atagaccagc cggccgcgct cgaccgtgac
     6481 ccgctcgacg ctgatctcga gcggcaagcc gaacagcgac aggcccggcg gcgccgccgg
     6541 cgcggagccg ccggcttcgt ccgcggcctc gggcggaccg agccgccagg tgacgcggcc
     6601 gtccgccagc cgctcgagct ggagtcgcgg ggcggccagg atcacgctgt cgatgcgcac
     6661 cttgcccgcg accagcggca ccagggcgac gcgcacgccg accgagtcga gcgagatcag
     6721 gtcgggatcg gcggcgccgt ccaggttggc gacccgcacc cgctcggccg acaatgtcgg
     6781 cgtcggcagc atggcgaacg cgatcgggcc gccgatcgcg atgtcgcggc cggtcagctt
     6841 cgccagttga tcgacgatct cgggcttgaa ccggttccag tcgacgaagc tcggcccaat
     6901 cagcagcgcc ccgatcacga ccagcagcac cgccaggaac ccgtagagca gtgacctcac
     6961 cgaacggtcc cctgacgatt gcgccggacg tgcctcaagg ttggctcccg cgggcttgcg
     7021 ccactcccga ataccgctaa tctaaccaat gcgttacggc cgttacatag ggacgggagc
     7081 gggacgcgag gctggggcgg gcgccggccg cggcgccgcg tcccgctgcg cggcggaggc
     7141 gccgatgggt gaggtagtga atctcgacct ataccgcaag cgcgcgcgga ccagggccgg
     7201 ccgcggcgcg gtgccggcaa ccccgggacc gcagggcgga ccgccggcgc gcgctgcggg
     7261 accggcaacc gaagaccggc cggaatgcga cgatgcgggc gccggcgacg gcgccccgcc
     7321 ggcgccgggc ggcccggaca ccgccgcggc cgattgagca aggacgcggc gcctcgggcc
     7381 cagcagcacc ataggcggcg cgcggcggca ggataggcgc ggcgctcggg cgatcgcgac
     7441 gcgcgaaggc ggcgtgcctg tgccgcgacc ggagcgccga atcgcggtgg cggaaatcga
     7501 tgtcacgcaa attatgtgtc gcgcccgccc gcgtcccgag cggcggagcg tccctacagg
     7561 cgcacgatct tgcccgggtt catgatgttg tcggggtcga tcgcgcgctt gatcgcgcgc
     7621 atcaccgaca acgcctcgcc gagctcttcg gtcaggaagt cgatcttgcc gtagccgacg
     7681 ccgtgctcgc cggtgcaggt gccctccatc gccagcgcgc gcgccaccag gcggtcgtgg
     7741 agccgggcgc cgaatgatat ctgcgcctgg tcgtcggggt cgatcatgta ggagacatgg
     7801 aagttgccgt cgccgacatg gccgacgatc gggcacttga acggcgccgt ggcgttgtcc
     7861 ttctcggtct cgaccacgca ctcggcgagc cgggagatcg gcacgcacac gtcggtgacc
     7921 atgcccttgg tgcccgggca cagcgcgcgc atggcgtaat aggcgtcgtg gcgcgcctgc
     7981 cacagcttgt tgcgctgctc gtggtcggcg gtccagcgga agtcgccgcc gccgtggtcg
     8041 gcggcgaact cctgcgcgtg ctcggcctgc tcgcgcaccg aggcctggct gccgtggaac
     8101 tcgtaaaaca gtgtgtaggc gaccgggtag tcggtcttgg agaaggcgtt gatcgcctcc
     8161 atcgacacct tgtcgatcag ctcgatgcgc gccaccggga tgcccgactg gatggtcacg
     8221 atcaccgcgt cgaccgcgtc gccgatcgag gggaacgagc aggtggcggc gctgatcgcc
     8281 tcggggatgc cggccaggcg caaggtcacc tcggtgatca cgccgagggt gccctccgag
     8341 cccacgaaca ggcgcgtcag gtcgtagccc gcggcagact tgcgcgcccg gcgggcggtg
     8401 cggatcacgc gcccgtcggg cagcaccacg gtcagcgcaa ggacgttctc gcgcatggtg
     8461 ccgtagcgca ccgcgttggt gccgctcgcg cgcgtcgccg ccatgccgcc gagcgaggcg
     8521 tccgcgcccg ggtcgatcgg gaagaacagg ccggtgtcgc gcagatattc gttgagctgc
     8581 ttgcgcgtca cgcccgcctg gacggtgcag tcgaggtcct cgggattgac ctcgaccacc
     8641 tcggtcatct ggctcacgtc gatgctgatg ccgccttcca gcgcggccac ctggccctcg
     8701 agcgaggtgc cggtgccgta ggcgatcacc ggcgcgcagt acctggcgca cagcgtgacc
     8761 gcgtcgctca cctcttcggt ggtgcgggcg aacaccaccg cgtccgggct gtagggggtg
     8821 tggtaggact cgtcccggcc gtgcagctcg cgcaccgccg ccgagaccga caggcgctcg
     8881 gcgaacagcg cgcgcagctc cgcgatcagc ggcgagcgcg ggtcgaggcg gcttcgagct
     8941 tgggcaggcg cgacggccat cgccggactc cctgacttaa gaccttgcag cgccggatat
     9001 agcatcggcg gcgccaaagg ttaagatgcg cgcctcgccc cggcagcgga gcccgcccgt
     9061 gacctcaccc gctcaccgcc gctcccagcc cctggtgctg caccgcgagc cggtgcgcga
     9121 ggcgtggctc gactacaacg gccacatgaa cgttgcctac tacctgctcg ccttcgacca
     9181 cgccagcgac gccttcgccg agcatatcgg catcggccag gcctatacgc gcaccagcgg
     9241 ccgctcgatc ttcatcctcg aatgccatgt cgcgtacttg cgcgaggtgg tcggcggcga
     9301 cccgttgcgc ttcaccaccc acctgctcgg cttcgacgac aagcgcatcc atttcgccca
     9361 cgccatgcat cacgacgtcc gcagctacca cgtggcgacc ggcgagctga tgctggtcca
     9421 tgtcgacctc gcccagcgca aggccaccgc catgcccgcc ccgacgctcg ccgcgctcgc
     9481 ccgcatcgcc tccgaccagc agggcctgga gcggccggtc ggcctgagcc gggtcatggg
     9541 cctgggggcg ggacggccct gagccgggcg ccgccgggcc tgtgaccgca gtcacgcgca
     9601 ggggcgcggg cgggcgctat atgcgctcat ggagctgaac caggcgctcg ccgataggag
     9661 ggccgagatg tcgatccctc cggtcaattt tcccttgccg tcgccgatcg cctcggcgct
     9721 gaaggcgatt cgggacgcca acgaaacggt cgccgaggtc gcgcaggacg tggccgagca
     9781 cggcgccgag ggcttcgccg agaacgcggt cgcgctgacc accgccgaga tctccgccaa
     9841 ggccgcgatc gcggttctcc gcaccgccgc cgagctcgag caggagacgc tcgacatcct
     9901 cgcctgagcg gcgcgccgcg gccccgcgcc tgagccggtt gaaaggcgca tccgatcatc
     9961 ccatcataag tggcgatcga cgccatcggg ggagcccaac atggccggaa gcccgtcgcc
    10021 tgcccgcaag ccgaccgtcg tcggccacga cgaccgctgg ctcaggctgt tgtacatggt
    10081 gatcttcgcg atcatcggtt acacggtgtt ctgggtggtc ctgttcctgg ccgcggtcca
    10141 gttcctgctc acctggatca acggcgagcc gaatcacaac ctgcgcgact tcgcgggcaa
    10201 gctcgatgct tatctccagg cggtgctcga cttcatcggc tatgcgaccg acgtggtgcc
    10261 gttcccgttg tcgccgctgg agacgagcct tgccagcgcg gcgccggccg ccgcccggcg
    10321 caggtcgcgc tcggccggcg cgcgcgccaa gggcggggcc gccgccaaga cggagcccgg
    10381 ggcggaggac ggctgaggcg ccgcgaacac cgctcggccg ggaccggggc ggcccgttta
    10441 gccggcgaat gattgccacc ctagcgccgt gacctcgtcg ggcgctagcg gactggccgg
    10501 gcttcgtcgc cggttgtcgc tcggtcgcga tcctcacctt tatctgtcgg tgcttgccct
    10561 cgtcatcggc gccgccgccg gctacggggc gatcgccttc aggctcggca tcgacgcgat
    10621 ccagcttctg ttctacggct cggcgacgca gcaggtgctg tcgatggcgc ggcacctgga
    10681 gtggtggcat cggctgctgg cgccggcgct gggcgggctc ggggtcggcc tgttcatcca
    10741 ctacgcgttg cccgagcgcc gcccgcacgg ggtcgccgac gtgatcgagg cgtgcgcgct
    10801 gcgtggcggg cggatgcggc tgcgcgacgg gctggcggcc gggttcgcca gcgccgcctc
    10861 gatcggcgtc ggcgcctcgg tcgggcgcga gggcccggtg gtccacctgg ccgccgccat
    10921 ggcgggcgcg ctcggcggcc gcctcgggct cgatcggggg caactgcgca ccctgctcgg
    10981 ctgcgccgtc gccagcggca tcgcggcctc cttcaacgcc ccgatcgcgg gcgtgttctt
    11041 cgcgctcgag gtggtggtcg gccactacgc gctgggcgcg tttgcgccgg tggtgatcgc
    11101 ggcggtgatc ggcaccatca tcacccgcgc ccagttcggc gacttcccgg cgttcgtggt
    11161 gccgggggcg gaaatcgcgt cgtactacga gatgccggcc ttcctgctgc tcggcgcggt
    11221 gtgcgcactg gtcgcgctgc tgttcatgtc ggggaccatg ttcaccgcca aggtggtggc
    11281 cagggtgccg gtcgcgggct acctgaagcc ggcggcgggc gggctgctca ccggcgcgat
    11341 cgcggcggcc tttcccgagg tcatcggggt cggctacgag accaccgacc tggcgctcac
    11401 cggcgcgttc acgctcgacg tgctgctgct cttggtggtg gccaagacca ccgccagcgt
    11461 catctgcctc ggctcgggct tcgccggcgg cgtgttctcg cccagcttgt gcatcggcgc
    11521 gctgaccggc gccgcgttcg gcatcattgc cacggccgtg gtgcccgagg cccaggccag
    11581 catcaacgcc tacagcctgg tcggcatggg cgcggtcgcc ggcgcggtgc tgggcgcgcc
    11641 gatctcgacc atcctgatca tcttcgagct gaccggcgac tatgcggtga cgatcgcggt
    11701 catgctatcg gtggtcaccg cctcgctgat cacccggcgc gtcgccgacg gctcgttctt
    11761 cacgctccag ctgcgcgcgc gcgggctgga tccggccagc gccgaggcgc gcgcgccgag
    11821 ccgcgcgatc accgtccggt cgctgatgag cgacagcttc cgcaccgtcg acgccgacac
    11881 cccggtgggc gagatccgcg agctgctgcg cagcgccccc gactacgacc tctacgtcac
    11941 cgacgacgcc ggccggctga ccggcgtggt gccgttcgac cggttgcgcg cgctgctgtt
    12001 cgacccgagc ccggacctcg atgcgcgcgc gagcagcctc gccgtcgagg cgctggacgt
    12061 gctggtgccg ggcgacacgc tcgacctcgc gctcgacatg ctcgagaccg gccaggcgcg
    12121 gcatctgccg gtggtcgagg accgcctcgc gcgccggctg atcggggtcg tgttccgcac
    12181 cgacgcgctg cacgcctaca accgcgccct gttgcgcgag cgcgcgctcg agcacggcag
    12241 cggttgagct ggtgctttcg tcgtccgacg agggcccatg gcccgtgcgc cgggggccgc
    12301 gcttgactat attggcgggc gaatcatggt tgacgcgttc gaccctttcg acctgaccca
    12361 gatcgcgccc gcggcgcgcc ccgtttcggc gcccggggca gagcgctatc tcgaggcgct
    12421 gaacgaggcc cagcggcgcg cggtcgagtg caccgagggg ccggtactgg tgctcgccgg
    12481 cgccggcacc ggcaagaccc gggtgctgac cacgcgcctc gcccatatcc ttgagtcgcg
    12541 gctcgcgcac ccgtccgaga cgctcgcggt caccttcacc aacaaggcgg cgcgcgagat
    12601 gcgcgagcgg gtcgagcgcc tgctcggccg ctcggtcgag ggctggcacc tcggcacctt
    12661 ccacgccgtc gccgcgcgca tcctgcgccg ccacgccgag tgcgtcgggc tcaagtcggc
    12721 cttcaccatc atcgacgccg acgaccaggt gcgcctgctc aagcagctgc tggcggcgga
    12781 gaacatcgac gagaagcgct ggccggcgcg cacgctcgcc gcgatcatcg gccgctggaa
    12841 ggaccgcggc ctgaccccgg acaaggtgtc gcgcacggac atcggcgagt tcgccgccgg
    12901 ccgcgcgctc gacctctacc gcgcctacca ggagcggctg gccaccctca atgccgccga
    12961 cttcggcgac ctgctgctgc acaacctgac cctgttcgcg cgcgatgcgc gcgtgctcgc
    13021 cgactaccag gaccggttcc gctacgtgct ggtcgacgag taccaggaca ccaacgtcgc
    13081 ccagtatctg tggctcaggc tcctggccca gaagcatcgc aacctgtgct gcgtcggcga
    13141 cgaggaccag tcgatctacg cctggcgcgg cgccgagatc ggcaacatcc tgcgcttcga
    13201 gcacgacttc ccgggtgcga cggtgatccg gctcgagcgc aactaccgct cgaccccgca
    13261 catcctcggc gccgccagcg gcctaatcgc gcacaacgag agccggctcg gcaagacgct
    13321 gtgggccgag ggcgaggccg gcgagacggt gcgcgtggtc ggggtgtggg acggcgagga
    13381 ggaggcgcgc ctggtcggcg accagatcga gtcgctgcac cggcgcgggc agcccttgcg
    13441 cgagagcgcg atcctggtgc gggcgagctt ccagacccgc gccttcgagg agcggttcat
    13501 ccagatcggc ctgccctacc gggtgatcgg cggcctgcgc ttctacgagc ggctcgagat
    13561 ccgcgacgcg ctcgcgtatc tgcgcgtgat cggacagccc gccgacgacc tcgccttcga
    13621 gcgcatcgtc aaccggccca agcgcggcat cggcgacgcc agcctgcaga ccttgaacct
    13681 tcacgcccgg acgcgcggaa tcgcgctgtt cgacgccgcc cgcgagctgg tcgagtccga
    13741 cgagctgcgc gggcgcgcgc gcgccgcgat gcgcgcgctc ttggacagcc tcgaccgctg
    13801 gcgcgcgctg gccgcgggcg gcaccgagct gccggcgctc gccgagaccg tattggacga
    13861 gtcgggctac accgggatgt ggcagcagga ccgctcggcg gacgcgcccg ggcggctcga
    13921 gaacctcaag gagctggtga tcgcgctcga ggagttcgac ggtctcggcg gcttcctcga
    13981 gcatgtcgcg ctcgtcatgg acaacgccgc cggcgccggc gaggacgggg tcaacctgat
    14041 gacgttgcac ggcgccaagg ggctcgagtt cgacaccgtg ttcctgcccg gctgggagga
    14101 ggagctgttc cccaaccggc gcgcgctcga ggacggcggc gtgcgcgcgc tcgaggagga
    14161 gcgccgcctc gcctatgtcg ggctgacgcg cgcgcgcaag cgggcgatgg tgctgtatgc
    14221 cgccaaccgg cacgccttcg gccaatggct gtcggcggcg ccgtcgcgct tcctcgccga
    14281 gctgcccgcg gcgcatgtgg cgcgcgacag cgtgcccgcc gcctattacg gcggcctctc
    14341 ggaggccgag gtggcggacc cctccggctt cgaccggccg gcgatcggcg cccggcgcag
    14401 caactgggcg cgctacgccg cgcgttacgg ggccgagccg gcgctgccgg tcgagacccg
    14461 cagccacgcg ccgggcacct tcgcgccggg cagccgcgtg ttccaccaga agttcggcta
    14521 cggcgaggtg cgcgcgatcg acggcaacaa gctcgagatc gcgttcgaca aggccggcac
    14581 caagaaggtg atggacagct tcgtcacccc ggcgtgagcg cgccggcgcg cgtattcgcc
    14641 cgccaagccg tcggcgtaca gccgtataag gcgaagaacg cgcggctgaa gtggctgcgg
    14701 ctgtcatagc cgaccgcggc gcccaccgcc ttgaccggcc gctccgaggt aacgagcagt
    14761 gccgccgccc ggcgcaaccg cacttccttg aggaagtcga tcggcccgcg gccgaaggcg
    14821 tggcggaagt gctcggcgaa cgtggagcgg ctcatgccgg cgacctcggc caggcgctcg
    14881 accgtgaacg ggcgccccgg atcgtcgagc atcgcccgga cgacgcagcc gatgcgttcg
    14941 tcctcaagcg cgatcagcca cggaacgcga cactcgccgc tctcgcaata gcggcgcagc
    15001 acgtgcacca ggcattgctg catcagcgcc cgcaccagca catcggtgcc gggctgggga
    15061 tgcgcgagct cggcgagcag ggcctcgaac gcgtcggcga acgcgcggtc ggccgccccg
    15121 cggaccacga tcggctcggg caggtagtcg aacagcccat ggccgcggtg gaaggtggcg
    15181 ccgatcgcgc cgcacgccag taccacgccg ccctcgcccg gcgcctcacc gtccgcggcg
    15241 aagcggtggc tgcgtcccgc cggcacgatc acggccgagc gccgctcgag cctgatcgcg
    15301 ggcaggccct cgatcgagag cccgcccgcg ccgtcgagca ggtagtggac gaccgcctcg
    15361 tcgcgccggc ccccatgcgc gctctcgccg gccgccagcc gcgagaccga gaacggctgc
    15421 accgccacct cgagcccatc gagcagccgc tcgagcagga tcatgacgca cctcgca
//
LOCUS       DASWHU010000321        23040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3505097, whole genome shotgun sequence.
ACCESSION   DASWHU010000321 DASWHU010000000
VERSION     DASWHU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 23040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23040
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505097"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VGB88_11955"
     CDS             <1..924
                     /locus_tag="VGB88_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648207.1"
                     /translation="GFAEYRWRFRTGLPWLEPRRFDQAAWHGEPLDGKAVLAWGEQGV
                     GEEVMFASLLPRLAEVARRVVVECDPRLVPLFRRSFADLSVVARATLAAPAARDVEIE
                     VQLAAGDLPAALRFGAGDDPAPAAYLVADPGQLAAAKRRLADLGPGLKVGIAWRSVGA
                     NMPFSRSKSSSLACWQAVLRTAGAVFVNLQHGDCARELAQARDRFGVQIHDDPSVDQR
                     ADLDAFAAQVAALDLVVTTSNTTAHMAGALGQEVWVVLPHVADWRWQHRRSDSLWYPR
                     ASLIRQPQPGDWAGALAEVARRLGARAGGAS"
     gene            921..2429
                     /locus_tag="VGB88_11960"
     CDS             921..2429
                     /locus_tag="VGB88_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648208.1"
                     /translation="MTAPAAAAAVSDGYRRALHAHRAGRLQDAAGLYAECLSAAPERA
                     DIAHLLGLSLLGLGDFAGGVAALQLSLSLDPEAAAVEHDLANALVSLGHAEAALDHFD
                     RALALDPGLVDAQVNRATALAKLGRHGEAAPALEAALVLAPRHAEALYRLGRVRAAAR
                     ERAGAIAAYQACLAVAPDHAEARYHLGDMQLAAGDFAAGWANYEARFHRPGNAVPMRP
                     FAAPRWDGQPLAGRCLLVWGEQGVGEEILFASRFADLAGLGGRIVVECDPRLVPLFER
                     SFPGVEAVGRRTPPDPRIAALAPDIQTPSGSLGRWLCAERAAFRPRPYLVADADRIAA
                     ARARHEALGPGLKVGIAWRSVGPNARYGRAKSTALADWAPLLATPGARFESLQYGDCA
                     REIADLGATCGATVHRDPAVDQAASLDDFAAQVAALDLVVTTSNTTAHMAGALGVETW
                     VLLPPAVDWRWQGAGEGSLWYPRARLFRRAPSEDWSAPIDAAVRLLAARTNG"
     gene            2422..4230
                     /locus_tag="VGB88_11965"
     CDS             2422..4230
                     /locus_tag="VGB88_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648209.1"
                     /translation="MADARLAGALDHHRQGRLDDAAVLYDRLIAENPANADAWHLRGV
                     VALQQGDARRALVLIGQALAAREDVADFHSNIGEAWRALGDLDRALGHYRRALALDAG
                     HLDAHNNAGVALKAKGALEAAEAHFRRVLAAEPDHSRALNNLGALLRARGLPAEAEQC
                     LRRAVAVAPSYADAHTNLGNALWALGREDEALGHYRRAIALDPGSAGALINLGTALEQ
                     QGDLESSSACYRRALAASPDNADAHFNLANNLAAEGLGEDARAAYLRAIAADAGHVGA
                     QVNLARLDLLDGRFAAGFDRWEWRWREPDCWRRILDSPVWDGAPLAGRTLAVWGEQGV
                     GDEIMLGSVLPDAIAAAGHVVVECDPRLVPLFARACPAAEFVARDSPPAPRLAAADID
                     LQCALGSLCRWLRRRPEDFAAPKPTLIADPAKVEAARARSGAPGPGLKVGIAWRSVPR
                     GAEAKTRRFSRAKSIPLDDWAPILTTPGVRFVDLQYGDTADELRRIEDRLGVRIHHDE
                     AVDQMASLEDFAAQIAALDMVVAASNTTVHLAGALGLRVWTMVPFVPDWRWQRGREDT
                     LWYPHMRLFRQPAPGAWAPVIARVAEELGRLAEGAA"
     gene            complement(4237..4977)
                     /locus_tag="VGB88_11970"
     CDS             complement(4237..4977)
                     /locus_tag="VGB88_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9648210.1"
                     /translation="MSPATPFRVYVGWDSREDIAYQVCRYSLLKRASVPIEVIPLRQE
                     ELRAQGLYWRERDPLASTEFTYTRFLVPQLAGRRGWALFCDCDFLWLADIAELLALID
                     DRCAVMCVHHDHNPPEMVKMDGAVQTRYPRKNWSSLVLFNCGHRANAKLTADVVSRES
                     GQYLHRFRWLDDAVIGALPETWNWLEGWSPRPDSGTPKVVHFTRGGPWFENWQDVTYA
                     ELWLDEERSHRRSLAAPAPRTVPAPAPA"
     gene            complement(5112..5492)
                     /gene="flaF"
                     /locus_tag="VGB88_11975"
     CDS             complement(5112..5492)
                     /gene="flaF"
                     /locus_tag="VGB88_11975"
                     /inference="COORDINATES: protein motif:HMM:NF009435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX9648211.1"
                     /translation="MAADRYARAIATTETDREREYRLLARANRLMVEAKKTGDLASLY
                     RAVLYNRQIWNTFLIDLTDERNALPRELKGALISIGIWVERFTDPVCEGAESVDDLID
                     VNRTIMEGLMPNAAAKPVPALPTV"
     gene            complement(5482..5877)
                     /locus_tag="VGB88_11980"
     CDS             complement(5482..5877)
                     /locus_tag="VGB88_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEX9648212.1"
                     /translation="MALKLTLKPNEKIIVNGAVLTNGPAKTTFSIENTAVILRQKDIL
                     TEENANTPAKRIYFCLQMAYLDREHERDYLEKANHLVRDFVAAVPTEEVRGILEPVGV
                     DVSKRNYFPALRQLRKLIAFEDKRLTYGR"
     gene            complement(5943..6767)
                     /locus_tag="VGB88_11985"
     CDS             complement(5943..6767)
                     /locus_tag="VGB88_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX9648213.1"
                     /translation="MAGQNSINTNVGALVALRNLVATNTELERVQNRVSTGLRVIGAQ
                     DNASIFAIAQGIRGDIKATAAVQQGLSQGKGISSVALSGATQVSNLLQDIKAKIVEGM
                     NPANTSEQQTILNADYAELVKQALSFIQNAEYNGQNLLKSGATTINVISDVSGGTLRV
                     RSQDLEGTAYSQLNAQNLANVTAASAALTQVNTVINTVNTALGQLGADDRALGYQDTF
                     LTTISDALDEGLGALVDADLAKESARLQALQVKQQLAVQTLSIANSSPQILLGLFQ"
     gene            complement(6934..7887)
                     /locus_tag="VGB88_11990"
     CDS             complement(6934..7887)
                     /locus_tag="VGB88_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648214.1"
                     /translation="MTRISTLSQNQYILQSTLATQSRLYDLRRQLASGNKAADFRDLA
                     SQAQSLSGALTRIARAEQYQANNVQTKAKLTLREAAVREISELAKDLKAQFIEAEGAE
                     DSRQLQADAENQLTRLVSILNSRDQNGNYMFAGSRTNLAPVTMTANGAPPSAFTFTFN
                     NDQVVEQARIDDGLVIDIGVLAGADAATPAAAFAQLFEVLNYFAAGVYPPPVAGTPAM
                     PQPGVPPVAGAPAQVIPVINAALDAVDQLDAALGLRLKLLEQVEVGLQEDVDLTREFA
                     GAINDADIAEVMTRISQDEVALEASYRITGELRLLSLVEFL"
     gene            complement(7909..10080)
                     /gene="flgK"
                     /locus_tag="VGB88_11995"
     CDS             complement(7909..10080)
                     /gene="flgK"
                     /locus_tag="VGB88_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX9648215.1"
                     /translation="MSLTGALNAALSGLNVSQASLALVAQNVANANNPDYRRKLAVQE
                     AQVTDGAVTGVQIAEIKRTIDEFLLRDVRASTSDFGRYDAQTPFLDRLQAFFGDPDSE
                     TTLSSRLDQVFAAFETAGTLNDNPTARSEVVFALDILGREIRRTFDQIQALRTDAEHQ
                     IVDDIADVNLALARIEVLNEKIVREVAAGRQPQDLLDQRDAQLAVIAQRLSVTSYVQG
                     DGALVVMAGRGMVLLDQEARVLDYSPANIAQPETVFDTITVRRASQLAGTGTPLGQDI
                     LSGRLRGLLDVRDGALADAASELGEFAARLADEVNRVHNDNVAFPPPAALTGSRNTGL
                     VAADAHNFTGRVTFSVMDPTVPGAGGWGVVASVTVDFDAGTVEQDFSGAPVVVPLTTI
                     QNVLDAVNGANGLNGAATLSLSGGVMSLAAATATNGVGTAQDATVPSDRGGRGFSHFF
                     ALNDLLVTTTPRMFEHGFAAADTHLFTGDTRFTVLDANGETVTTVTVTPVAGTFAGLV
                     TQLDTALTVGGTPYASFALDANGRLVMTEAAGYTVLASDSGPPATSDRGGTGIGLASL
                     LGLGADKVQRIASNIAVNPAILADNDRLAIAKLQGAALNDPGATPGDARGAQALAAIA
                     TTAVAINGAGNLSARTTTLSAYVGALLGDAAIQARNADGQRAEFKLLSDTLSNMFQSI
                     AGVNVDEELATMIVLQNAYSASARLITAVSQMFDDLMAIAT"
     gene            10399..10761
                     /locus_tag="VGB88_12000"
     CDS             10399..10761
                     /locus_tag="VGB88_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648216.1"
                     /translation="MSLVQPGKATGPPASARDPVAVAGGPRTPTAARAGEPQPSVTTA
                     VARDFSEHLLEASRPLAHTRLSILYDKDAELFITRQVEKGSGEVVHQFPYEEQVARIR
                     YFLDQMREDREARLDEVA"
     gene            complement(10789..11244)
                     /locus_tag="VGB88_12005"
     CDS             complement(10789..11244)
                     /locus_tag="VGB88_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648217.1"
                     /translation="MTAEPHADARIETLIDVLAQLSSVLGEETAAVRARDYRRLEGLT
                     ERKGQLAATYGRALGGLAPHHRAGRGIEVRLSARLRTRAREFMASLETNAHALGAAKT
                     AHERLIRSISEAVAATARPARGYSRSGAWRAGGRPGRTEMVPIALNRKV"
     gene            complement(11241..11558)
                     /locus_tag="VGB88_12010"
     CDS             complement(11241..11558)
                     /locus_tag="VGB88_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEX9648218.1"
                     /translation="MIDTIAGTALDTPRPPALLPRIDPKAAPDQLRKVAREFESVFLG
                     QMLETMFEGIETDGPFGGGHGERVFRSLLLQEYGRTISAQGGIGIADAVVRQLLAVQE
                     TQE"
     gene            complement(11563..12729)
                     /locus_tag="VGB88_12015"
     CDS             complement(11563..12729)
                     /locus_tag="VGB88_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX9648219.1"
                     /translation="MDDDRTHRRPCRLRPRLAGAALIGALAVVAAGIGAAAAASVRIK
                     DVSDFEGIRDNMLVGYGLVVGLDGTGDTLNNSPFTRQSLEAMLERLGVNTRGGNVKTD
                     NVAAVMVTATLPPFARQGTRIDVTVNSLGDAENLKGGTLMVTPLLGADGEVYAVAQGQ
                     VSVSGFAAQGAAGTSVTQGVPTSGRISNGAIVEREVGFELADLDRVTVALRNPDLTTA
                     GRIAIAINAKLGRAHARALDPGTVMVSVPESYRHRMVELMTDIEQLRIEPDQVARVVV
                     DEVSGTIVMGENVRISTVAIAQGNLTIRITETPQVSQPGAFAPDSTSTVVVERTEIEI
                     EEGEDNKLAVLPEGVSLQELVDGLNALGVGPRDIISILQAIKAAGALQADLELM"
     gene            12925..13350
                     /locus_tag="VGB88_12020"
     CDS             12925..13350
                     /locus_tag="VGB88_12020"
                     /inference="COORDINATES: protein motif:HMM:NF022227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HEX9648220.1"
                     /translation="MKIVTERIGAPAAPKRPARAGRAGDGAAFATMVETPSSEDAQGP
                     AAVHPGTPADALLMAQQLPEGELGRRRNLQRGRTILAQLESLRLALIAGAIPRAQLRQ
                     LLAMVELERVPAADPRLAAVLDEIELRARVELAKYGQFD"
     gene            13898..14263
                     /gene="dksA"
                     /locus_tag="VGB88_12025"
     CDS             13898..14263
                     /gene="dksA"
                     /locus_tag="VGB88_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX9648221.1"
                     /translation="MGARQREYFRRKLVTWRDEIRAESRETIEHLREQNSQEPDFADR
                     ATIETERLIELRTRDRERKLLSKIDAALLRIEEGSYGFCLETGDPISLKRLEARPIAT
                     LSIDAQERHERLEKTYRDD"
     gene            complement(14285..15043)
                     /gene="flgH"
                     /locus_tag="VGB88_12030"
     CDS             complement(14285..15043)
                     /gene="flgH"
                     /locus_tag="VGB88_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX9648222.1"
                     /translation="MIRRPAPRLRPLLAVSAALLLGACSAAERLGQVGRAPDFDPIIN
                     PIQKPDYAPVTMPMPAPEIASHQANSLWRTGARAFFKDQRAAQVGDILTIEIAIDDGA
                     TISNETTRSRDNLESSKVSSLFGYEASFAQVLPEAVDPTNLLSIDTDLSNKGSGEIKR
                     GEQIDLKLAAIVTQILPNGNMVIEGHQEVLVNYDLRALNLRGIVRPEDISASNTISYE
                     KIADARISYGGRGQLHDVQQPRYGSQVLDVINPF"
     gene            complement(15075..16052)
                     /gene="flgA"
                     /locus_tag="VGB88_12035"
     CDS             complement(15075..16052)
                     /gene="flgA"
                     /locus_tag="VGB88_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX9648223.1"
                     /translation="MTPFRLRTARHAAACVVVLAATAAAAAGPEPRLRPVVEVEGAVV
                     TLGDLFEGAGAGAGEPVLAAPAPGERLLVSIRQAFDLANDHGLGWRPVAPFDHVVVTR
                     AWRMLDRAEVVRALEASLRALGAGDTLRIELPPAPTRLAVARGAIAELAVEDATWQAE
                     TGRFQATLVVDDDAGPARRHALSGRAWPMVELPVLNARLRRDDVIAAGDISWKTFRAD
                     RVPDNALDRADQLVGLSPVRTVTPGRPLRADDLGAPIIVDKGALVTMSLVTERMKLVA
                     TGRAQESGALGDVIRVRNAHSKLVVEAIVTGPNRVSVTSPGLRVVAHEG"
     gene            complement(16078..16863)
                     /gene="flgG"
                     /locus_tag="VGB88_12040"
     CDS             complement(16078..16863)
                     /gene="flgG"
                     /locus_tag="VGB88_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX9648224.1"
                     /translation="MRSLSIAATGMLAQQLNVEVISNNIANMQTTGYKRQRPEFQDLL
                     YQSVRQVGSTSSDTGTVVPSGIQLGLGVRTASVYRIIEQGTILGTNATFDLAIQGKGW
                     FQVQLPSGETGYTRAGSFQLSPSGEIVTADGYTVLPAITIPPEAIQVSINATGDVEIK
                     LAGQSAPSNVGTLTLASFPNEAGLESIGNNLMLESASSGGAVTGIPGSGGLGTLLQGF
                     LESANVNAVAEITSLITAQRAYEMNSKVITTSDEMMAAISQLR"
     gene            complement(16903..17610)
                     /locus_tag="VGB88_12045"
     CDS             complement(16903..17610)
                     /locus_tag="VGB88_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HEX9648225.1"
                     /translation="MDTTTYVSLSVQNALQRQMDIIAHNIANAATGAFKSEKPIFVEV
                     LDENNDVAYVEDYGVARDMQAGRLEVTGGPLDVAVQGDAFLAVQVGDEVRYTRNGHLQ
                     LDAEGELSTSTGYAVLDIDQRPIEIPPGVDGITISPDGTVSGGDGPLARIGMVAFDNP
                     LDMERAPDSLYVTAQAPVPPRDASLVQGALEMSNVSPVLQLTEMIEVLRAYQRNAELA
                     ENEHQLQLKTIDTAIQA"
     gene            17863..18399
                     /locus_tag="VGB88_12050"
     CDS             17863..18399
                     /locus_tag="VGB88_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX9648226.1"
                     /translation="MADKDAAAAADDGAEEAPRKRRIAGKKIVLFGVLPALVLIGAGV
                     GLLTSGLFGGGADEAGEAMPGDQGMAEARSPDQAVFYDLPPMLVNLNSGARQSSFLKI
                     QVSLELGGPDAVPEVEAMLPRIVDNFQVYLRELRPDELSGTKGVARLKEELLVRVNRA
                     VEPVTVYDILFKEILVQS"
     gene            18422..19480
                     /gene="fliM"
                     /locus_tag="VGB88_12055"
     CDS             18422..19480
                     /gene="fliM"
                     /locus_tag="VGB88_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX9648227.1"
                     /translation="MAEPASPEETAAEETADGDEADAGAGGAGHATRVLSQEEIDSLL
                     GFDEHGAGGQDGAGIHAVLNSALVSYERLPMLEVVFDRMVRMMSTSLRNFTSDNVEVS
                     LDRITSARFGDYLNSIPLPAMLSVFKAREWDNYGLITVDSALIYSIVDVLLGGRRGTA
                     AMRIEGRPYTTIERNLVERMICVILQDMSQSFEPLSRVTFEYDRLETNPRFATIVRPG
                     NAAIVASLRIDMEDRGGRVELVIPYATLEPVRELLLQMFMGEKFGRDSIWESHLATEL
                     WVTDVDLEAVLDEQVMSLDQVMKLKVGDTIILDADPSSEIELRCGGIPLMVGTMGRSG
                     NQIAIHVDRTLKRLGRRQ"
     gene            19477..19887
                     /locus_tag="VGB88_12060"
     CDS             19477..19887
                     /locus_tag="VGB88_12060"
                     /inference="COORDINATES: protein motif:HMM:NF039465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HEX9648228.1"
                     /translation="MTLGLTVDAALALLLVVTIAYCAAVHRRLGAIRAAQADMRALAD
                     RLAAAGAQAEAGLGQLRVVAAEAADGLGPRIKTARRLAGDLDMLCHRADKLAERLDRT
                     QAPSRARAAGLAAQHAARRSRSEHALIEALKAKP"
     gene            19904..20581
                     /locus_tag="VGB88_12065"
     CDS             19904..20581
                     /locus_tag="VGB88_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648229.1"
                     /translation="MRIRLLPVTMFLGGLLLTVKLGALVSGGGPVTAGIQVADSQAQQ
                     ADAATAPGEGDAPAGEPDSELAGLEPPPALPPLAPPTEFTQAELDVLQNLRERRETLD
                     ARETELDLRQSTIEAAEHNVEGRIEDWKRLKAEVEQLLTRYEKAQNDELDTLAAYYEK
                     MKPKDAARVFDTLDLPYLIEIVGRMKAAKVADVIGKMDTLKAKELTIELARQDQDAGA
                     GAGTATP"
     gene            20678..21064
                     /locus_tag="VGB88_12070"
     CDS             20678..21064
                     /locus_tag="VGB88_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9648230.1"
                     /translation="MAVDKDMSVLIVDDLESMLSTIRSLLAQLGFHNVEQATDGRAAL
                     AKARTKPPGLIISDWNMEPMGGLELLKEVRADASLRHVPFIIVSAEARAEAIIAAKRA
                     GADTYILKPFGAETLRAKLASVLGAF"
     gene            21089..21760
                     /locus_tag="VGB88_12075"
     CDS             21089..21760
                     /locus_tag="VGB88_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648231.1"
                     /translation="MDDDAESKKAPQKDPTLALFLGLYLILLAFFVLLNTMATPKDDR
                     IKAVLGSLLATFSTEILNRVTPTEFAASIGEDVALQEYHRDIRTFFEVAVPLARVEYY
                     SAGTVMRVRLPADQLFEPESIRVRGDREELWSRIQRAMQRDVRDKRYELEFIVFTGPF
                     LAGEAPSGRLLEVARAGAFARLLEARAVRRDLIVIGARPGNPDEIEMTFQVRVLEPVK
                     ADLPG"
     gene            21765..22526
                     /locus_tag="VGB88_12080"
     CDS             21765..22526
                     /locus_tag="VGB88_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX9648232.1"
                     /translation="MADKAIIDEAPPPPEPDDDEPSGITIDSEAWMLSFGDLVSLMLV
                     FFVMLFAMSTLEDEKYEAIVSALAQSFNPTAVVDMPVPSQDQDIPKTDVKELYSLDYL
                     RALLEEKLAGDAMLADIGLHQLNDRLVISLPADQLFTATGARLADGARETVARMGTVI
                     RFVGNRVDVDGHTDPSPVADASFPSNWELSLARAMAVADELRAGGYVDTINAFGLADT
                     HFAEISLDLRVDERYRRARRVDIVVRKDRAEEGTP"
     gene            22523..>23040
                     /locus_tag="VGB88_12085"
     CDS             22523..>23040
                     /locus_tag="VGB88_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648233.1"
                     /translation="MTARAARWLAALAALLCAAGPAHAQDTSPTEVPVQAVAEGDSAM
                     IVFLWPSPVAFDARVAGRSLQVRFDRPIVPRLSEVRTKLGAFVESVGVEDDGRMLIMT
                     LTGAFSLSSARQDNAVVLTLTRAEGGRQAGEELPVVGVRVGEHEAFGRVVFDWSREVG
                     YRVERDGDTAVLR"
BASE COUNT         3202 a         7974 c         8453 g         3411 t
ORIGIN      
        1 ggtttcgccg agtatcgctg gcgcttccgc accggcctgc cgtggctcga gccgcgccgc
       61 ttcgaccagg ccgcctggca cggcgagccg ctcgacggca aggcggtgct cgcgtggggc
      121 gagcaggggg tgggcgagga ggtcatgttc gcgtccttgc tgccgcggct cgccgaggtg
      181 gcgcgccggg tcgtggtcga gtgcgatccg cgcctggtgc cgctgttccg ccgctcgttc
      241 gccgatctct cggtggtcgc gcgagccacg ctcgcggcgc cggcggcgcg cgacgtggag
      301 atcgaggtcc agctcgccgc cggcgacctg ccggccgcgc ttcgcttcgg cgccggcgac
      361 gacccggcgc cggccgcata tctggtcgcc gaccccgggc agctggcggc ggcgaagcgg
      421 cggctcgccg acctcggccc ggggctcaag gtcggcatcg cctggcgctc ggtgggcgcg
      481 aacatgccgt tcagccggtc caagtcgagc agcctcgcct gctggcaggc ggtgctgcgg
      541 accgcgggcg cggtgttcgt caatctccag catggcgact gcgcgcgcga gcttgcccag
      601 gcccgcgacc gctttggggt gcagattcac gacgacccga gtgtcgacca gcgcgccgac
      661 ctcgacgcct tcgccgcgca ggtggcggca ctcgacctgg tggtgacgac ctccaacacc
      721 accgcccaca tggccggtgc gctgggccag gaggtgtggg tggtgctgcc ccatgtcgcc
      781 gactggcgct ggcagcaccg gcgcagcgac agcctgtggt acccgcgcgc atcgttgatc
      841 cgccagcccc agcccggcga ctgggccggc gcgctggcgg aggtcgcccg ccgcctcggg
      901 gcgcgcgccg gcggcgcgtc gtgaccgcgc cggcggccgc ggcggcggtc agcgacggct
      961 accggcgggc gcttcatgcc catcgcgccg ggcggctcca ggacgccgcc gggctctacg
     1021 ccgaatgcct gagcgcggcc cccgagcgcg ccgatatcgc ccatcttctg gggctcagcc
     1081 tgctcgggct cggcgacttc gccggcggcg tcgccgcgct gcagctgtcg ctcagcctcg
     1141 atccggaggc cgcggcggtc gagcacgacc tcgccaatgc gctggtctcg ctcggccatg
     1201 ccgaggctgc gctcgaccat ttcgaccgcg cgctggcgct cgatcccggc ttggtcgatg
     1261 cccaggtcaa ccgcgccacg gcgctcgcca agctcggccg gcacggcgag gccgcgccgg
     1321 cgctcgaagc cgcgctcgtg ctggcgccgc gacatgccga ggcgctctac cggctcggcc
     1381 gggtacgcgc ggcggcgcgc gagcgggcgg gcgcgatcgc cgcctatcag gcgtgcctgg
     1441 cggtcgcccc cgaccacgcc gaggcgcgct atcacctcgg cgacatgcag ctcgcggcgg
     1501 gcgacttcgc cgccggctgg gccaattacg aggcccgctt ccaccgcccc ggcaacgccg
     1561 tgccgatgcg cccgttcgcc gcgccgcgct gggacggcca gccgcttgcc gggcggtgcc
     1621 tgctggtctg gggcgagcag ggggtgggcg aggaaatcct gttcgcctcc cggttcgccg
     1681 acctcgccgg cctcggcggg cgcatcgtcg tcgaatgcga cccgcgcctg gtgccgctgt
     1741 tcgagcgctc gtttccgggc gtcgaggcgg tcgggcggcg gacgccgccg gacccgcgca
     1801 tcgcggcgct ggcgcccgat atccagaccc cgagcggcag cctcggccgc tggctgtgcg
     1861 ccgagcgcgc cgcgttccgc ccgcgcccct atctggtcgc cgacgcggac cgcatagcgg
     1921 ccgcccgcgc gcgccatgag gcgctcggcc cggggctcaa ggtcggcatc gcctggcgca
     1981 gcgtcggccc caacgcccgc tacggccgcg ccaagtcgac ggcgctcgcc gactgggcgc
     2041 cgctgctggc gacgccgggc gcgcggttcg agagcctgca atacggcgat tgcgcgcgcg
     2101 agatcgcgga cctgggcgcg acctgcgggg ccacggtcca ccgtgacccg gcggtcgatc
     2161 aggcggcgag cctcgacgac ttcgccgccc aggtggcggc gctcgacctg gtcgtcacca
     2221 cctccaacac caccgcccac atggcgggcg cgctcggcgt cgagacctgg gtgctgctgc
     2281 cgccggcggt cgactggcgc tggcaagggg cgggcgaggg gagcctgtgg tatccgcgcg
     2341 cgcgcctgtt ccggcgcgcg ccgtccgagg actggtcggc gccgatcgac gcggcggtgc
     2401 ggctgctggc ggcgcgcacc aatggctgac gcgcggctcg ccggggcgct cgaccatcac
     2461 cgccagggcc ggctcgacga cgccgcggtg ctgtacgatc gcctgattgc cgagaatccg
     2521 gccaacgccg atgcctggca tctgcgcggg gtggtggcgc tgcaacaggg cgatgcccgg
     2581 cgcgcgctgg tgctgatcgg ccaggcgctg gcggcgcgcg aggacgtcgc cgacttccac
     2641 agcaacatcg gcgaggcctg gcgcgcgctc ggcgatctcg accgcgcgct cggccactac
     2701 cggcgggcgc tggcgctcga tgccggccat ctggatgcgc acaacaatgc cggcgtggcg
     2761 ctgaaggcga agggcgcgct ggaggcggcg gaggcgcatt tccggcgcgt gctggcggcc
     2821 gagcccgacc acagccgcgc gctcaacaac ctcggtgccc tcctgcgcgc gcgcgggctg
     2881 cccgccgagg ccgagcagtg cctgcgccgg gcggtggcgg tggcgccgtc ctacgccgat
     2941 gcgcacacca atctcggcaa cgcgctgtgg gcgctcggcc gcgaggacga ggcgctcggc
     3001 cattaccggc gcgcgatcgc gctcgacccc ggaagcgcgg gcgcgctgat caacctcggc
     3061 accgcgctcg agcagcaggg cgacctcgag tcgtcgtccg cctgttaccg ccgcgcgctg
     3121 gcggcgagcc cggacaacgc cgacgcccac ttcaacctgg ccaacaacct tgccgcagag
     3181 ggcctgggcg aggacgcgcg cgccgcctac ctgcgggcga tcgccgccga tgccggccat
     3241 gtcggggcgc aggtaaacct cgcccggctc gatctcctgg acggccgctt cgccgccggc
     3301 ttcgaccgct gggagtggcg ctggcgcgag cccgactgct ggcggcgcat cctcgattcg
     3361 ccggtctggg acggcgcgcc gctcgccggg cgcacgctcg cggtgtgggg cgagcagggg
     3421 gtcggcgacg agatcatgct gggatcggtg ctgcccgatg cgatcgccgc cgccggccat
     3481 gtggtggtcg agtgcgaccc gaggctggtg ccgctgttcg cgcgcgcctg tcccgcggcc
     3541 gagttcgtcg cccgcgactc gcccccggcg ccgcggctcg ccgccgccga catcgacctc
     3601 caatgcgcgc tcggcagcct gtgccggtgg ctcaggcgca ggcccgagga cttcgccgcg
     3661 cccaagccca ccctgatcgc cgatccggcc aaggtcgaag cggcgcgggc ccgcagcggc
     3721 gcgcccggcc cgggcctcaa ggtcggcatc gcctggcgca gcgtcccccg cggggctgag
     3781 gccaagaccc ggcgcttcag ccgcgcgaag tcgatcccgc tcgacgactg ggcgccgatc
     3841 ctgaccaccc cgggtgtgcg gttcgtcgac ctccagtacg gcgacaccgc ggacgagctg
     3901 cggcggatcg aggaccgatt gggggtgcgc atccaccacg atgaggccgt cgaccagatg
     3961 gcgagcctcg aggacttcgc ggcccagatc gcggccctcg acatggtggt ggcggcgtcg
     4021 aacaccaccg ttcacctggc cggcgcgctc ggcctgaggg tgtggaccat ggtgccgttc
     4081 gtgcccgact ggcgctggca gcgcgggcgc gaggatacgc tctggtatcc gcacatgcgc
     4141 ctgttccgcc agccggcgcc aggcgcatgg gcgccggtga tcgcgcgcgt ggccgaggag
     4201 ctcggccggc tcgccgaggg cgcggcctga gccggctcag gccggcgccg gcgcgggcac
     4261 ggtgcgcggc gcgggcgcgg ccagcgatcg gcggtgcgac ctctcctcgt cgagccagag
     4321 ctcggcatag gtcacgtcct gccagttctc gaaccacggc ccgccgcggg tgaagtggac
     4381 caccttgggg gttcccgagt cgggccgcgg gctccagccc tcgagccagt tccaggtctc
     4441 gggcagcgcg ccgatgacgg cgtcgtcgag ccaccggaag cggtgcagat actggccgct
     4501 ctcgcggctg accacgtccg ccgtgagctt cgcgttggcc cggtggccgc agttgaacag
     4561 caccagcgac gaccagttct tgcgcgggta gcgggtctgc accgcgccgt ccatcttcac
     4621 catctcgggc ggattatggt cgtggtggac gcacatcacc gcacagcggt cgtcgatcag
     4681 cgccagcagc tcggcgatgt cggcgagcca caggaagtcg cagtcgcaga acagcgccca
     4741 gccccgccgc ccggcgagct gcggcaccag gaagcgggtg taggtgaact cggtcgaggc
     4801 gagcgggtcg cgctcgcgcc agtacagccc ttgcgcgcgc aactcctcct ggcgcagcgg
     4861 gatcacctcg atcggcacgc tcgcccgctt aagcagcgaa tagcgacaga cctgataggc
     4921 gatgtcctcg cggctgtccc agccgacgta gacgcggaac ggggtggcgg ggctcatgat
     4981 tgtcctgtcc tgcgtgtgcg gtagcagtcg atatagggcg cggcgcggtc gcgaatagcg
     5041 cgcaacgcgg cctcggcccc gacacgcaag cgccgtgcca ggtggcaggc ggcgcgggac
     5101 ggcgagtgcg gttatacggt ggggagcgcg ggcaccggct tggccgcggc gttgggcatc
     5161 aggccttcca tgatcgtgcg gttgacgtcg atcaggtcgt cgacgctctc cgcgccctcg
     5221 cacaccggat cggtgaacct ctcgacccag atgccgatcg agatcagcgc gcccttcagc
     5281 tcgcgcggca gcgcgttgcg ctcgtcggtg aggtcgatca ggaaggtgtt ccagatctgg
     5341 cggttgtaca gcaccgcgcg gtacaggctg gcgaggtcgc cggtcttctt cgcctcgacc
     5401 atcagccggt tggcgcgcgc cagcaggcgg tattcgcgtt cgcggtcggt ctcggtggtg
     5461 gcgatggcgc gggcgtaacg gtcagcggcc ataggtcagc ctcttgtcct cgaaggcgat
     5521 cagcttccgc aattgcctga gcgccgggaa atagttccgc ttcgacacgt ccacgcccac
     5581 cggctccagg atgccgcgca cctcttccgt gggcactgcg gcgacgaagt ctcgcaccaa
     5641 gtggtttgcc ttctctaaat agtctcgctc gtgctcgcgg tcgagatagg ccatctgcag
     5701 gcagaaatag atgcgcttgg ccggcgtgtt ggcgttctcc tcggtcagga tgtccttctg
     5761 gcgcaagatg acggcggtat tctcgatgct gaaggtggtc ttggccggac cgttggtcag
     5821 gacggcgccg ttgacgatga tcttttcgtt cggtttcagc gtcagcttca gcgccattca
     5881 ggtccccgcg cctgaagaat gacggggccg gtgcgcggca ccggccccgc cagggaaggc
     5941 cgctactgga acaggccgag caagatctgc ggcgaggagt tggcgatgct gagcgtctgc
     6001 accgccagtt gctgcttcac ctgcagggct tgcaaccgcg ccgattcctt ggccaggtcg
     6061 gcgtcgacca gtgcccccag gccttcgtcg agcgcgtcgg agatcgtggt caggaaggtg
     6121 tcctgatagc cgagcgcgcg atcgtcggcg ccgagctggc cgagcgccgt gttgaccgtg
     6181 ttgatcaccg tgttgacctg ggtcagcgcg gccgaagcgg cggtcacgtt ggccaggttc
     6241 tgggcattga gctggctgta ggccgtgccc tccaggtcct gcgagcgaac ccgcagggtg
     6301 ccgccggaca cgtccgagat cacattgatc gtggtggcac cggacttgag caggttctgg
     6361 ccgttgtact cggcgttctg gatgaagctc agcgcctgct tgaccagctc ggcgtagtcg
     6421 gcgttcagga tcgtctgctg ctccgacgta ttggccgggt tcatgccctc gacgatcttg
     6481 gccttgatgt cctgaagcag gttcgagacc tgggtcgcgc ccgacagcgc caccgaggag
     6541 atgcccttgc cctgggacag gccctgctgg accgccgcgg tcgccttgat gtcgccgcgg
     6601 atgccctggg cgatggcgaa gatcgaggcg ttgtcctggg cgccaatcac ccgcaggccc
     6661 gtcgacacgc ggttctggac tctttcgagc tcggtgttgg tggcaaccag gttcctgagc
     6721 gcgacgagcg ctccgacatt ggtattaatg gaattttgac cagccatgaa aaggctcctc
     6781 gttctaaggc ttgaaattac atgccttctg gcacggacaa ccgccgcgaa ccggcgcctc
     6841 gtggggactc ggcgtcgccg cgacggtcgc ggataatagg gtagatcggc gccccgcgat
     6901 atcaagcccg cggcgcgcgg cccgcgcccg gtcctagagg aactcgacca ggctcaagag
     6961 gcgcagctcg ccggtgatgc ggtacgaggc ctcgagcgcg acctcgtcct ggctgatgcg
     7021 ggtcatcacc tcggcgatat cggcatcgtt gatggcgccg gcgaactccc tggtcaggtc
     7081 gacgtcctcc tgcaggccga cctcgacctg ctcgagcagc ttgagcctga gcccgagcgc
     7141 ggcatcgagc tggtcgaccg catcgagcgc ggcgttgatc accgggatca cctgggcggg
     7201 cgcgccggcc accggcggca ccccgggctg cggcatcgcc ggcgtgccgg cgaccggcgg
     7261 cgggtagacg ccggcggcga agtagttgag cacctcgaac agctgggcga aagcggcggc
     7321 gggggtggcc gcatcggcgc cggccagcac cccgatgtcg atcaccaggc cgtcgtcgat
     7381 gcgcgcctgc tcgaccacct ggtcgttgtt gaaggtgaag gtgaaggcgg acggcggcgc
     7441 gccgttggcg gtcatggtca ccggcgccag gttggtgcgc gagccggcga acatgtagtt
     7501 gccgttctgg tcgcgcgaat tgaggatgct gaccaggcgg gtgagctggt tctcggcgtc
     7561 ggcctgaagc tggcgcgagt cctccgcccc ctcggcctcg atgaactggg ccttgaggtc
     7621 cttggccagc tcggagatct cgcgcaccgc cgcctcgcgc aaggtgagct tggccttggt
     7681 ctgcacgttg ttggcctggt actgctcggc gcgcgcgatc cgggtcagcg cgccgctcag
     7741 gctctgcgcc tggctggcga gatcgcgaaa gtccgccgcc ttgttgccgg aggcgagctg
     7801 gcggcgcagg tcgtagagcc gcgactgagt ggcgagcgtc gactgcagga tgtactggtt
     7861 ctgcgacagg gtcgagatgc gggtcatggg ggttctcgtc gcgaagcgtc aggtggcgat
     7921 ggccatcagg tcgtcgaaca tctggctgac cgcggtgatc aggcgcgccg aggccgaata
     7981 ggcgttctgc agcacgatca tggtcgcgag ctcctcgtcg acgttcaccc cggcgatcga
     8041 ctggaacatg ttcgacaggg tgtccgacag gagcttgaac tcggcgcgct ggccgtcggc
     8101 gttgcgggcc tggatcgcgg cgtcgccgag cagcgcgccg acataggccg acagcgtcgt
     8161 agtccgcgcc gacaggttgc ccgcgccgtt gatcgcgacc gcggtggtgg cgatcgcggc
     8221 cagcgcctgg gcgccgcgcg cgtcgcccgg ggtcgctccc ggatcgttca gcgccgcgcc
     8281 ctgcagcttg gcgatcgcca gccggtcgtt gtcggcgagg atcgccgggt tgaccgcgat
     8341 attgctcgcg atgcgctgca ccttgtcggc gccgaggccg agcagcgagg caaggccgat
     8401 gccggtgccg ccgcggtcgg aggtcgccgg cggcccggaa tcgctcgcca gcaccgtata
     8461 gcccgcggcc tcggtcatca ccaggcggcc gttggcgtcg agcgcgaagc tcgcgtaagg
     8521 cgtgccgccg acggtcaagg cggtatcaag ctgggtgacc aggcccgcga aggtgccggc
     8581 cacgggcgtg accgtgaccg tggtcacggt ctcgccgttg gcgtcgagga cggtgaagcg
     8641 ggtgtcgccg gtgaacaggt gcgtgtcggc ggcggcgaag ccgtgctcga acatgcgcgg
     8701 cgtggtcgtg accaagaggt cgttgagggc gaagaaatga gagaagccgc gcccgccgcg
     8761 atcgctgggg acggtcgcgt cctgggcggt gccgacgccg ttggtggcgg tggccgcggc
     8821 gagcgacatg acgccgcccg agagcgacaa ggtcgccgcg ccgttgaggc cgttggcgcc
     8881 gttgaccgcg tcgagcacgt tttggatggt ggtgagcggc accaccaccg gcgcgccgct
     8941 gaagtcctgc tcgaccgtgc cggcgtcgaa gtcgaccgtg accgaggcga cgacgcccca
     9001 gccgccggcg ccgggcacgg tcgggtccat caccgagaac gtcacccggc cggtgaagtt
     9061 gtgcgcatcg gccgcgacca ggccggtatt gcgcgacccg gtcagcgccg ccggcggcgg
     9121 aaaggcgaca ttgtcgttgt ggacccggtt gacctcgtcg gccaggcgcg cggcgaactc
     9181 gccgagctcg ctcgcggcgt cggcaagcgc gccgtcgcgg acgtccagga gcccgcgcag
     9241 ccggccgctg aggatgtcct ggccgagcgg cgtgccggtg ccggcgagct ggctcgcgcg
     9301 gcgcaccgtg atggtgtcga acacggtctc cggctgggcg atgttggccg ggctgtagtc
     9361 gagcacccgc gcctcctggt ccagcagcac cattcctcgc ccggccatga ccaccagggc
     9421 gccgtcgcct tgcacgtagc tggtgacgct caggcgctgg gcgatcaccg ccagctgggc
     9481 gtcgcgctgg tcgagcaggt cctgcggctg gcgcccggcg gcgacctcgc gcacgatctt
     9541 ctcgttgagc acctcgatgc gcgccagcgc caggttgacg tcggcgatgt cgtcgacgat
     9601 ctgatgctcg gcgtcggtgc gcaaggcctg gatctggtcg aaggtccgcc ggatctcgcg
     9661 ccccaggatg tcgagcgcga acaccacctc gctgcgcgcg gttgggttgt cgttgagggt
     9721 gccggcggtc tcgaaggcgg cgaacacctg gtcgagccgg ctcgacaggg tggtttctga
     9781 gtcggggtcg ccgaagaacg cctgcaggcg gtcgaggaac ggcgtctggg cgtcgtagcg
     9841 gccgaagtcg gacgtgctgg cgcgcacgtc gcggagcagg aactcgtcga tggtgcgctt
     9901 gatctcggcg atctggacgc cggtgaccgc accgtcggtg acctgcgcct cctggaccgc
     9961 cagcttgcgc cggtagtcgg ggttgttggc gttggcgacg ttctgcgcta ccagcgcgag
    10021 cgaggcctgg ctgacgttga gcccgctgag cgctgcgttg agggcgccgg tgagcgacat
    10081 ctgacggggt ctcctcgagg ctgtcggggg ttgatcctgc aaatgccggg ccacgccggc
    10141 ggggccgggc aaggagcggg caaagtctcg gaatgccgcg cttttccggg gcgccgcggc
    10201 gaccggccct agacccgccg gcgtcgcccg cgcggcagtt cttgccactg ccgtccgggc
    10261 cgggccgggc ggcttgactt ggccggatgc gagctatctt cactgggtgt tcccggaacc
    10321 gggaaccccc cgctggcttg tttcgaaacc gaaggttttc gggccatgat gaaggctgtc
    10381 ggggcgggga caggcattgt gtcgttggtg cagcccggca aggcgaccgg gccgccggcg
    10441 tctgcgcgcg atccggtcgc ggtcgcgggt gggccacgca cgcccaccgc cgcgcgtgcc
    10501 ggcgagccgc agccgagcgt gaccaccgcg gtggcgcgcg atttctccga gcatctgctc
    10561 gaggcctcgc ggccgcttgc ccacacccgg ctgtcgatcc tgtacgacaa ggacgccgag
    10621 ctgttcatca cccgccaggt cgaaaagggc tcgggcgagg tggtgcacca gttcccttac
    10681 gaggagcagg tggcgcgcat ccgctacttc ctcgaccaga tgcgcgagga ccgcgaggcg
    10741 cgcctggacg aggtcgcctg agcgcggcgc cggcgcgccc gcctgccgtt agaccttccg
    10801 gttcaacgcg atcggcacca tttcggtgcg ccccggccgg cccccggcgc gccaggcgcc
    10861 cgagcgcgag tagccgcgcg cgggccgcgc ggtcgccgcg accgcctcgc tgatcgacct
    10921 gatcagccgc tcatgcgccg tcttcgcggc gccgagggcg tgcgcgttgg tctcgagcga
    10981 ggccatgaac tcgcgcgcgc gcgtccgcag cctggcgctg agccgcacct cgatgccgcg
    11041 cccggcgcga tggtgcggcg caagcccgcc cagcgcacgg ccataggtcg cggcgagctg
    11101 ccccttccgt tcggtgagcc cctcgagccg gcgatagtcg cgcgcgcgca ccgccgcggt
    11161 ctcctcgccg agcacggaag agagctgggc gagcacgtcg atcagggtct cgatacgcgc
    11221 gtcggcgtgg ggctctgcgg tcattcttgc gtctcctgca ctgcgagcaa ctggcggacc
    11281 accgcgtcgg cgatgccgat gccgccttgc gcggaaatgg tccggccgta ctcctggagc
    11341 agcagcgagc ggaacacccg ctcgccatgg ccgccgccga acggcccgtc ggtctcgatg
    11401 ccctcgaaca tggtctcgag catctggccg aggaacactg actcgaactc ccgggcgacc
    11461 ttgcgcagct ggtccggggc ggccttgggg tcgatccggg gcagcagcgc cggcggccgg
    11521 ggggtatcga gagcggtgcc tgcgatcgtg tcgatcatcg ccttacatca gctccaggtc
    11581 cgcctgcagc gcgccggcgg ccttgatcgc ctgcaggatc gagatgatgt cgcgcgggcc
    11641 gacgccgagc gcgttcaggc cgtcgaccag ctcctgcaag ctgactcctt cgggcagcac
    11701 ggcgagcttg ttgtcctcgc cttcctcgat ctcgatctcg gtgcgctcga ccaccaccgt
    11761 cgaggtgctg tcgggggcga acgcgcccgg ctgcgacacc tgcggggtct cggtgatgcg
    11821 gatggtcagg ttgccctggg cgatggcgac ggtcgagatg cgcacgttct cgcccatcac
    11881 gatggtgccc gagacctcgt cgaccaccac ccgcgccacc tggtcgggct cgatgcgcag
    11941 ctgctcgatg tcggtcatca gctcgaccat ccggtggcgg tagctctcgg gcaccgacac
    12001 catcaccgtg ccggggtcga gcgcgcgcgc gtgcgcccgg ccgagcttgg cgttgatcgc
    12061 gatcgcgatg cgcccggcgg tggtcaggtc ggggttgcgc agcgccaccg tgacccggtc
    12121 gaggtcggcc agctcgaagc cgacctcgcg ctcgacgatg gcgccgttgg agatccgccc
    12181 cgaggtcggc acgccctggg tgacgctggt gccggcggcg ccctgggcgg cgaagccgga
    12241 gaccgagacc tggccctggg cgaccgcgta gacctcgcca tcggcgccga gcagcggggt
    12301 gaccatcaag gtgccgccct tgaggttctc ggcgtcgccc agcgagttga cggtgacgtc
    12361 gatgcgcgtg ccctggcggg cgaacggcgg cagggtggcg gtgaccatga ccgcggcgac
    12421 gttgtcggtc ttgacgttgc cgccgcgggt gttgaccccc agccgctcga gcatcgcctc
    12481 gaggctttgg cgggtgaacg gcgagttgtt gagcgtgtcg ccggtgccgt ccaggccgac
    12541 caccaggccg tagccgacca gcatgttgtc gcggatgccc tcgaagtcgc tcacgtcctt
    12601 gatgcgcacg ctcgctgccg cggccgcgcc gatgccggcc gcgaccacgg cgagggcgcc
    12661 gatcagcgcc gcgccggcaa ggcgcgggcg caagcggcac gggcggcggt gtgtccggtc
    12721 gtcgtccatc gctgggctcg tccaatgtcc tggtccgccc atgccaggac cgtgccaacc
    12781 gcggaaccgg caccggcagc cgccgccggc gccggcacga ggcgcggcgc gggcaaaaac
    12841 tgccgcccgc cgcggcccgc gcgcgattaa acgcttgtta acaacgccgt gccagggtcg
    12901 gcgcgcaaac acgggccgag ggcaatgaag atcgtcaccg agcgcatcgg cgcgcccgcc
    12961 gcgcccaagc gtccggcccg cgccggccgg gccggcgacg gcgcggcgtt cgcgaccatg
    13021 gtcgagacgc cgtcgagcga ggacgcccag ggcccggccg cggtccaccc cggcacgccc
    13081 gccgacgccc tgctcatggc ccagcagctg cccgagggcg agcttggccg gcggcgcaac
    13141 ctgcagcgcg gacgcaccat cctcgcccag ctcgagtccc tgcggctcgc cctgatcgcg
    13201 ggcgcgatac cgcgtgcgca gctgcgccaa ttgctcgcaa tggttgaatt agagcgtgtc
    13261 ccggcggccg atccgcgcct cgccgcggtg ctcgacgaga tcgagttgcg ggcccgcgtc
    13321 gagctcgcca aatacggcca gttcgactga catcgtcgcc gtttgcgaat tccctggagg
    13381 cgcttctggc cggttgcagg gccaagggcg gattcatata ctccgccgcc aaaaaaaatc
    13441 gggccgcgcc aagcccgtga taacgaggag cttcatggca gcgcagggaa aggccaagcg
    13501 gacaggcagg agcaaaacca caagccgcgc gcgggacaag gtcaagaccc tggcgcatgg
    13561 cggggccaag gtcaaaggcg gcggacgggt caaggcggcg gtgccgacca aggccaagac
    13621 cgcggtgcgg cgcagcgcca agcacgcaac gccggccaag accgcccgcc gggtagccgc
    13681 caagatcgcc ggccgggtag ccgccaagac caaggcgcgc gcgccggcca agtcgagggc
    13741 caaggccccg gccaaggcgg cggccaagtc ggcgcccaag tcggcgccca aggcggcggc
    13801 cagacaggcc ggccggtcgg gcaacggcgc cggccgccgg cgcaagcggg gaaccagcat
    13861 tcccgccggc taccgcccga gcgaccaaga ggagttcatg ggcgcgcgtc agcgcgagta
    13921 cttccgccgc aagctggtca cttggcgcga cgagattcgt gccgagtcgc gcgagaccat
    13981 cgagcacctg cgcgagcaga acagccagga gccggacttc gccgaccggg cgacgatcga
    14041 gaccgagcgg ctgatcgagc tcaggacccg cgaccgcgag cgcaagctcc tgtccaagat
    14101 cgacgccgcg ctgttgcgga tcgaggaggg cagctacggg ttttgcctgg agaccggcga
    14161 tccgatcagc ctcaagcggc tcgaggcgcg cccgatcgcg accctcagca tcgacgccca
    14221 ggagcgccac gagcgcctgg agaagaccta ccgcgacgat tgagccggcg ccgctgcggg
    14281 tgggtcagaa cgggttgatg acgtcgagca cctggctgcc gtagcgcggc tgctggacgt
    14341 cgtgcagctg gccgcggccg ccgtaggaga tgcgcgcgtc ggcgattttc tcgtagctga
    14401 tggtgttgct ggccgagatg tcctcggggc gcacgatgcc gcgcaggttg agcgcgcgca
    14461 ggtcgtagtt gaccagcacc tcctggtggc cctcgatcac catgttgccg ttgggcagga
    14521 tctgggtgac gatcgcggcg agcttgaggt cgatctgctc gccgcgcttg atctcgccgc
    14581 tgcccttgtt gctcaggtcg gtgtcgatgc tgagcaggtt ggtcgggtcg accgcttcgg
    14641 gcagcacctg ggcgaagctc gcctcgtaac cgaacaggct gctcaccttc gagctctcga
    14701 ggttgtcgcg cgagcgcgtg gtctcgttgg agatggtggc gccgtcgtcg atcgcgatct
    14761 cgatggtcag gatgtcgccg acctgggcgg cgcgctggtc cttgaagaac gcgcgtgcgc
    14821 cggtccgcca cagcgagttc gcctggtggc tggcgatctc gggcgccggc atcggcatgg
    14881 tcaccggcgc gtagtccggc ttctggatcg ggttgatgat cgggtcgaag tcgggcgcgc
    14941 gcccgacctg gccgaggcgc tcggccgccg agcaggcgcc gagcagcagc gcggccgaga
    15001 ccgcgagcaa gggccgaagg cgaggggcgg ggcggcggat catgatcgca tgtccctggc
    15061 tcgcggcggg cgagtcaacc ctcgtgcgca accacgcgca ggccgggcga ggtgaccgag
    15121 acccggttcg gtccggtgac gatggcttcc accaccagct tgctgtgcgc gttgcggacc
    15181 cggatcacgt cgccgagcgc gcctgattcc tgcgcccgcc cggtcgccac cagcttcatc
    15241 cgctcggtga ccaggctcat ggtgaccagc gcgcccttgt cgacgatgat cggcgcgccg
    15301 agatcgtcgg cgcgcagcgg ccggccgggg gtgacggtgc gcaccggcga cagcccgacc
    15361 agctggtcgg cgcggtcgag cgcgttgtcc ggcacgcggt cggcgcggaa ggtcttccag
    15421 ctgatgtcgc cggccgcgat cacgtcgtcg cggcgcaggc gggcgttgag caccggcaat
    15481 tcgaccatcg gccaggcgcg cccgctgagc gcatggcggc gcgccgggcc ggcgtcgtcg
    15541 tcgaccacca gcgtcgcctg gaagcggccg gtctcggcct gccaggtcgc gtcctcgacc
    15601 gcgagctcgg cgattgcgcc gcgcgccacc gcaagcctgg tcggcgccgg cggcagctcg
    15661 atgcgcaggg tgtcgccggc gccgagggcg cgcagcgacg cctcgagggc gcgcaccacc
    15721 tcggcgcggt cgagcatgcg ccaggcgcgg gtgacgacca cgtggtcgaa cggcgccacc
    15781 ggccgccagc cgaggccgtg atcgttggcg aggtcgaacg cctggcggat cgacaccagc
    15841 agccgctcgc ccggcgccgg cgcggccagc accggctcgc cggcgccggc gcccgcgccc
    15901 tcgaacaggt cgccgagggt gaccaccgcg ccctcgacct cgaccaccgg gcgcagcctg
    15961 ggctcgggcc cggccgccgc cgcggcggtc gcggccagca ccacgacgca ggctgcggcg
    16021 tgacgtgccg tgcgcagtcg gaaaggcgtc atcggccggt ctcctcagcc accgtgttca
    16081 gcgcagctgg ctgatggcgg ccatcatctc gtccgaggtg gtgatcacct tcgagttcat
    16141 ctcgtaggcg cgttgggcgg tgatcaggct ggtgatctcg gcgaccgcat tgacgtttgc
    16201 gctctcaagg aagccttgca aaagggtgcc gaggccgccg ctgccgggga tgccggtgac
    16261 cgcgccgccc gacgaggcgc tctccagcat caggttgttg ccgatcgatt cgaggccggc
    16321 ctcgttggga aagctcgcca aggtcaaggt gccgacattg gacggcgccg actggccggc
    16381 gagcttgatc tcgacgtcgc cggtggcatt gatgctgacc tggatcgcct ccggcggaat
    16441 ggtgatcgcc ggcagcacgg tgtagccgtc ggcggtgacg atctctcccg acgggctgag
    16501 ctggaacgag ccggcacggg tatagccggt ctcgccgctc ggcagctgga cctggaacca
    16561 gcccttgccc tggatggcga ggtcgaaggt ggcgttggtg cccaggatgg tgccctgctc
    16621 gatgatccgg tagaccgagg cggtgcgcac gccgaggccg agctggatgc ccgacggcac
    16681 caccgtgccg gtatccgagg aggtcgagcc gacctggcgc acgctctggt acagcaggtc
    16741 ctggaactcg ggccgctggc gcttgtagcc ggtggtctgc atgttggcga tgttattcga
    16801 gatcacctcg acgttgagct gctgggccag catgccagtg gcggcgatac tcagggatct
    16861 catggcggca atcctcgact tggctcgtcg cgcgcgtccg gcctaggcct ggatcgcggt
    16921 gtcgatcgtc ttcagctgca gctggtgctc gttctcggcc agctcggcgt tgcgctggta
    16981 ggcgcgcagc acctcgatca tctcggtcag ctggagcacc gggctcacgt tcgacatctc
    17041 gagcgcgccc tggacgaggg atgcatcgcg cggcggcacc ggtgcctggg cggtcacgta
    17101 caggctgtcg ggcgcgcgct ccatgtcgag cgggttgtcg aaggcaacca tgccgatgcg
    17161 cgccagcggg ccgtcgccgc ccgagaccgt gccgtcgggg ctgatggtga tgccgtcgac
    17221 cccggggggg atctcgatcg ggcgctggtc gatgtcgagc acggcgtagc cggtcgaggt
    17281 gctgagctcg ccctcggcgt cgagctgcaa atggccgttg cgggtgtagc gcacctcgtc
    17341 gccgacctgg accgcgagga acgcatcgcc ctggacggca acgtcgagcg gcccgccggt
    17401 cacctcgagc cggccggcct gcatgtcgcg ggcgacgccg tagtcctcga cataggcgac
    17461 gtcgttattc tcgtcgagga cctcgacgaa gatcggcttc tcggacttga acgcgccggt
    17521 cgcggcgttg gcgatgttgt gggcgatgat gtccatctgg cgctggagcg cgttctgcac
    17581 cgacagcgat acataggtgg tggtgtccat cggcgccgtc cgcctcgtct catgcccgcg
    17641 gcgcgggctc acgcgccgag ggatgcaggc cccgtgccaa ccgcggaacg cggcgccgcc
    17701 aaggcccttg gcgggcgcgc gcgccggcgc ggggcaagcc gtgccgggca gttcttgccg
    17761 ccggcgactc gggcgcaggc gcgcaaccgc ttgttaacca agcccgccta gagtgcacgc
    17821 ggaataaggc ggacggccgc gttgcggccg ggcgagggga cgatggccga caaggacgca
    17881 gctgcagccg cagacgacgg cgccgaggag gcgcccagga agcggcggat cgcgggcaag
    17941 aagatcgtgc tgttcggggt gctgccggcg ctggtgctga tcggcgccgg ggtcggcctg
    18001 ttgacatcgg gcctgttcgg cggcggcgcg gacgaggccg gcgaggccat gcccggcgac
    18061 caggggatgg ccgaggcgcg cagtccggac caggcggtgt tctacgacct gccgccgatg
    18121 ctggtgaacc tcaacagcgg cgcccgccag tcgagcttcc tcaagatcca ggtcagcctc
    18181 gagctcggcg gcccggacgc ggtgcccgag gtcgaggcga tgctgccgcg gatcgtcgac
    18241 aacttccagg tctacctgcg cgagctgcgc cccgacgagc tcagcggcac caagggcgtg
    18301 gcgcggctga aggaggagct tctggtgcgc gtcaaccggg cggtcgagcc ggtcacggtc
    18361 tacgacatcc tgttcaagga aatcctggtc caaagctgac tttgcggggc ggatcggagc
    18421 gatggcggaa ccggcatcac cggaggagac cgcggcggaa gagaccgccg acggcgacga
    18481 ggccgacgcc ggcgccggcg gtgccggcca cgcgacccgg gtcctcagcc aggaggagat
    18541 cgacagcctg ctcggcttcg acgagcacgg cgcgggcggg caggacggcg ccggcatcca
    18601 cgccgtgctc aactcggcgc tggtctccta cgagcgcctg cccatgctcg aggtggtgtt
    18661 cgaccgcatg gtgcgcatga tgtcgacgtc gctgcgcaac ttcacctcgg acaatgtcga
    18721 ggtgtcgctc gaccgcatca cctcggcgcg gttcggcgac tatctcaact cgatcccgct
    18781 gcccgccatg ctgtcggtgt tcaaggcgcg ggagtgggac aactacgggc tgatcacggt
    18841 cgatagcgcg ctcatctatt cgatcgtcga cgtgctgctg ggcgggcgcc gcggcaccgc
    18901 cgcgatgcgc atcgaaggcc gcccctatac caccatcgag cgcaacctgg tcgagcgcat
    18961 gatctgcgtg atcctgcagg acatgagcca gtcgttcgag ccgctgagcc gggtgacgtt
    19021 cgagtacgac cggctggaga ccaacccgcg cttcgccacc atcgtccgcc cgggcaacgc
    19081 ggcgatcgtc gcctcgctgc gcatcgacat ggaggaccgc ggcgggcgcg tcgagctggt
    19141 gatcccgtac gccacgctcg agccggtgcg cgagctgttg ctgcagatgt tcatgggcga
    19201 gaagttcggc cgcgattcga tctgggaaag ccacctcgcc accgagctgt gggtcaccga
    19261 cgtcgacctc gaggcggtgc tcgacgagca ggtcatgtcg ctcgaccagg tcatgaagct
    19321 gaaggtcggc gacaccatca tcctcgacgc cgacccgagc tcggagatcg agctgcgctg
    19381 cggcggcatc ccgttgatgg tcggcaccat ggggcggtcg ggcaaccaga tcgcgatcca
    19441 cgtcgaccgg acgctcaagc ggctcgggcg gcggcaatga ccctcgggct cacggtcgat
    19501 gcggcgctgg cgctgctgct ggtggtgacc atcgcctact gcgccgccgt ccaccgccgc
    19561 ctcggcgcga tccgggcggc acaagccgac atgcgcgcgc tcgccgaccg gctcgccgcc
    19621 gccggcgccc aggccgaggc cgggctcggc cagctgcgcg tggtcgccgc cgaagccgcg
    19681 gacgggctcg ggcccaggat caagaccgcg cgccggctcg ccggcgacct cgacatgctg
    19741 tgccaccgcg ccgacaagct cgccgagcgg ctcgaccgga cgcaggcgcc ctcgcgcgcg
    19801 cgcgccgccg ggctcgcggc ccagcacgcg gcccggcgct cgcgctcgga gcacgccttg
    19861 atcgaggcgt tgaaggccaa gccgtgatcc gcggagacag gcgatgcgga taaggctgct
    19921 gccggtcacc atgttcctcg gcgggctgct gctgacggtc aagctgggcg cgctggtcag
    19981 cggcggcggg ccggtcaccg ccggcatcca ggtcgccgat tcccaggccc agcaggccga
    20041 tgccgcgacc gcgccgggcg agggcgatgc gccggccggc gagccggatt cggagctggc
    20101 cggcctcgag ccgccgcccg cgttgccgcc gctcgcgccg ccgaccgagt tcacccaggc
    20161 cgagctcgac gtgctgcaaa acctgcgcga gcgccgcgag acgctggatg cgcgcgagac
    20221 cgagctcgac ctgcgccagt ccacgatcga ggccgccgag cacaacgtgg aaggccgcat
    20281 cgaggactgg aagcggctca aggccgaggt cgagcagttg ctgacccgct acgagaaggc
    20341 ccagaacgac gagctcgaca ccctcgccgc ctattacgag aagatgaagc cgaaggacgc
    20401 cgcgcgggtg ttcgacaccc tcgacctgcc ttacctgatc gagatcgtcg ggcgcatgaa
    20461 ggcggccaag gtggccgacg tcatcggcaa gatggacacg ctcaaggcca aggagctgac
    20521 catcgagctg gcgcgccagg accaggacgc cggcgccggc gccggcaccg ccacgccatg
    20581 atcgccgcgg ccgcgccgcc gcccgcgggc cgccgctgcg acggcgccgg gactgggcta
    20641 tcatcaccgc ttgcggcgcg agcgcggagg ccgggcgatg gccgtggaca aggacatgtc
    20701 ggtgctgatc gtggacgatc tcgagtccat gctgagcacc atccgcagcc tgctcgccca
    20761 gctcggcttc cacaatgtcg agcaggcgac cgacgggcgt gcggcgcttg ccaaggcgcg
    20821 cacgaagccg cccggcctga tcatctcgga ctggaacatg gagccgatgg gcgggctcga
    20881 gctgctcaag gaagtgcgcg ccgacgcctc cctcaggcac gtgccgttca tcatcgtcag
    20941 cgccgaggcc cgggccgagg ccatcatcgc cgccaagcgg gcgggcgccg acacctacat
    21001 cctcaagccg ttcggcgccg aaaccctgcg cgcgaagttg gcctccgtgc tcggcgcgtt
    21061 ctgagccgcg cggcggcgtg agccggacat ggacgacgac gccgagtcaa aaaaggcgcc
    21121 ccaaaaggac ccgaccctcg ccctgttcct gggcctgtac ctgatcctgc tcgccttctt
    21181 cgtgctgctc aacaccatgg cgacgcccaa ggacgaccgg atcaaggcgg tgctcggcag
    21241 cctgctcgcc acgttctcga ccgagatcct caaccgggtc acgccgaccg agttcgccgc
    21301 cagcatcggc gaggacgtgg cgctccagga gtaccaccgc gatattcgca cctttttcga
    21361 ggtcgcggtg ccgttggcgc gggtcgagta ctactcggcc ggcaccgtga tgcgcgtccg
    21421 cctgcccgcc gaccagctgt tcgagcccga gagcatccgc gtccgcggcg atcgcgagga
    21481 gctgtggtcg cgcatccagc gggcgatgca gcgcgacgtc agggacaagc gctacgagct
    21541 ggagttcatc gtcttcaccg gcccgttcct cgccggcgag gcgccgtcgg ggcggctgtt
    21601 ggaggtggcg cgggcgggcg cgttcgcgcg cctgctcgag gcgcgcgccg tgcgccgcga
    21661 cctgatcgtg atcggcgccc ggcccggcaa cccggacgag atcgagatga ccttccaggt
    21721 gcgcgtcctc gagccggtca aggccgacct gcccgggtag tccggtggcg gacaaggcga
    21781 tcatcgacga ggccccgccg ccgcccgagc cggacgacga cgagccgtcg gggatcacca
    21841 tcgactccga agcctggatg ctgtcgttcg gcgacctggt gtcgctgatg ctggtgttct
    21901 tcgtcatgct gttcgcgatg tcgaccctcg aggacgagaa atacgaggcg atcgtcagcg
    21961 cgctggccca gagcttcaac ccgaccgcgg tggtcgacat gccggtgccg tcgcaggacc
    22021 aggacatccc caagacagac gtcaaagagc tctacagcct cgactacctg cgcgcgctgc
    22081 tggaggagaa gctcgccggc gatgccatgc tcgccgacat cggcctgcac cagctcaacg
    22141 accggctggt gatctcgctg cccgcggacc agctattcac cgccaccggc gcgcgcctcg
    22201 ccgacggcgc ccgcgagacg gtggcgcgca tgggcaccgt catccgcttc gtcggcaacc
    22261 gggtcgacgt cgacggccac accgacccct cgccggtggc cgacgcctcg ttcccgtcca
    22321 actgggagct gtcgctcgcg cgcgcgatgg cggtcgcgga cgagctgcgc gccggcggct
    22381 atgtcgatac catcaacgcc ttcggcctcg ccgacaccca tttcgccgag atctcgctcg
    22441 acctgcgggt cgacgagcgc tatcgacggg cgcgcagggt cgatatcgtg gtgcgcaagg
    22501 accgggccga ggagggcacg ccgtgaccgc acgggccgcg cgatggctgg ccgcgcttgc
    22561 cgcgctcctg tgcgcggccg ggcccgcgca cgcgcaggac acgagcccca ccgaggtgcc
    22621 ggtgcaggcg gtcgccgagg gcgacagcgc gatgatcgtg ttcttgtggc cgagcccggt
    22681 cgcgttcgat gcgcgcgtgg cggggcggtc gctgcaggtc cggttcgacc ggccgatcgt
    22741 gccgcgcctg tcggaggtgc ggaccaagct cggcgcgttc gtcgagagcg tcggcgtcga
    22801 ggatgacggc cgcatgctga tcatgacctt gaccggcgcg ttcagcctgt cgagcgcgcg
    22861 ccaggacaac gccgtggtgc tgaccctgac cagggccgaa ggcggccgcc aagcgggcga
    22921 ggagctgccg gtggtgggcg tgcgggtcgg cgagcacgag gcgttcggcc gggtggtgtt
    22981 cgactggtcg cgcgaggtcg gttatcgggt cgagcgcgac ggcgacaccg cggtgctgcg
//
LOCUS       DASWHU010000322        24183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_577499, whole genome shotgun sequence.
ACCESSION   DASWHU010000322 DASWHU010000000
VERSION     DASWHU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577499"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /gene="mobB"
                     /locus_tag="VGB88_12090"
     CDS             complement(<1..256)
                     /gene="mobB"
                     /locus_tag="VGB88_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX9648234.1"
                     /translation="MKVIGIAGWSGSGKTTLVTRLLPELVGRGLSVSTVKHAHHEFDI
                     DHPGKDSYQHRVAGATEVLVSSARRRALIRELRGETEAGLD"
     gene            complement(273..866)
                     /gene="pgsA"
                     /locus_tag="VGB88_12095"
     CDS             complement(273..866)
                     /gene="pgsA"
                     /locus_tag="VGB88_12095"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9648235.1"
                     /translation="MPNLLTLSRVAVIPVFVAAFFLPAQAADWATFVLFVAASATDYA
                     DGWLARRREQETALGRFLDPIADKLLVAAALVMLLWLARAPALAALVILCREIMVSGL
                     REFLAGREVTLPVSRLAKWKTAIQMLSLCVLLAAGAAPALGLGTDQALALGGGLLWLA
                     ALLTAVTGLDYLRVGLRHMAARDRPAAQSLRAAGRRR"
     gene            complement(892..2748)
                     /gene="uvrC"
                     /locus_tag="VGB88_12100"
     CDS             complement(892..2748)
                     /gene="uvrC"
                     /locus_tag="VGB88_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9648236.1"
                     /translation="MIRDALAALPPGPGVYRMLNAHGKALYIGKARSLRKRVAAYANP
                     IRQSTRIARMIGETAAVEVVTTHTEAEALLLEANMIKRLKPHYNIVLRDDKSHPYILL
                     TGDSDWPQLLKHRGARSRPGRYFGPFASAGAVNRTLNALQRAFPLRTCSDNVFAGRTR
                     PCLQYQIKRCTAPCVGLIAAEDYAEIVDQTRDFLSGRSQAVQDRLAESMQKASDALEF
                     EGAAVLRDRIRALAHIQAHQGINVEAIGDADVIAADQQAGQTCVQVFFFRSGRNYGNR
                     AHFPSHARDEPVAGVLAAFVAQFYDSRTPPPLILASDALDGQALIEEALGLRAERRVR
                     ILCPQRGDKRQLVDNAVANAREALARRLAESASQRRQLDGIAQLLAMETAPERIEVYD
                     NSHISGTRAVGAMIAAGPQGLVKGGYRKFNIRGARDGDGAGFAPGDDYAMMREVLRRR
                     FTRLQKEDPERAQGQWPDLVLLDGGAGQLSSALEVFADLGIEVGPGNLTVAAIAKGPD
                     RDAGRERIHLPGRAPFMLPPNTPASYFLQRLRDEAHRFAIGGHRARRSRALGLSELDQ
                     IAGVGPKRKRALLNRFGSARAVGEAGLADLDKVEGVSKQMARAIYDHFHGAG"
     gene            complement(3182..3592)
                     /gene="folB"
                     /locus_tag="VGB88_12105"
     CDS             complement(3182..3592)
                     /gene="folB"
                     /locus_tag="VGB88_12105"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9648237.1"
                     /translation="MTPPGKVHPIPQPVPRTVRRRLFIRDLELRCAIGAYAHERGKRQ
                     RVRIGVELEMAEPDAPHDDRLAAVVSYDDIAQGVRRVVDEGHINLVETLAERIADLCL
                     ADRRVQVVRVRIEKLDVLPDAAGAGVEIERRLAE"
     gene            complement(3601..4377)
                     /locus_tag="VGB88_12110"
     CDS             complement(3601..4377)
                     /locus_tag="VGB88_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9648238.1"
                     /translation="MTADRRGLALVTGAGTRIGRALALDLAAHGWAVAVHHRRSGDAA
                     AEVVDRIVAGGGHAVALAADLAVEAEAAGLVDAAAAAAAGAVTCLVNNAAAFEYDSVA
                     TATRRSWDLHLDTNLRAPFVLSQAFARALPEGLSGTIVNMIDQRVWNLSPHFVSYTLS
                     KAGLWTLTRTLALALAPRIRVNAIGPGPVLASARQSAESFAAQVARTPLRRQVGLNEI
                     CAALRFVVDAPSLTGQMIALDGGQHLGWQHPGEPGAPEPE"
     gene            4474..6225
                     /locus_tag="VGB88_12115"
     CDS             4474..6225
                     /locus_tag="VGB88_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX9648239.1"
                     /translation="MESQFLPQVLVLLIAAVVIVFAFERLRLSPVLGYIVAGAAIGPY
                     GLKLISGVEETRALAELGIVFLLFMIGLELPYERLAAMRRYVLGLGVLQVAITGVIFG
                     GLARWLGQSLESSVVIGVGLALSSTAIALQLLVLRGEQTTPLGRASIAILITQDLLVV
                     PMLVLIPGLGDEAGSLTGALGEALLQAAVVLAAIILAGRLLLRHVFRLIARARQAELL
                     TATALVVILGTAAVTAQVGLSLALGAFIAGLLLAESEYRHHVEADIAPFRGILLGLFF
                     MTVGMSVDIGRLPAELSTVAALVAVLLAVKAAVLFALVSLTRLGAAAAVRTALLLCQG
                     GEFAFIMFTLAIEHGVMAQATYDLLILVVGVTMAMTPGLAWIGRALARHMMAGEADRH
                     GGLAAGAAELEGHVVILGFGRVGQTIATMLKAIDIPYVAVDLEPRRVIEGRARGLPVF
                     YADASRPQVLRRANVERACAAVVAVDNPANAVRVVDAIRRAGPEVGIYARARDRTEMQ
                     RLEAAGATVVIPDTLEASLQLGGQLLSRVGMSDTDIDRLCAELRTEGYGRLADMIPAR
                     TPPAAAERTADAPRAAE"
     gene            complement(6242..7492)
                     /locus_tag="VGB88_12120"
     CDS             complement(6242..7492)
                     /locus_tag="VGB88_12120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9648240.1"
                     /translation="MTRLGSVAAWGLAATAFLVVPLAALAPKGLTPLGVVAGLFAIAA
                     LRARAQPLPPAFLWLTAAIAALLAWCWLSALWALDPGLAVRRAEVLTLIAVVGPLILA
                     GTLALPADRQSWLGAGLCAGLALGVAFMAADAMLDGALRHAIRDDPGYYSMSSLNRGA
                     TVAVLVVWPAALWLWRRGVRADRRWMRAAAPALALSCVGLLALLESESSMAALAVGAA
                     TFAVVALARGIGTRLLAAALALGTMAAPALSLTILSPDRVLAWTDRLGSTAVHRLFIW
                     HFTAERILERPWLGWGLDAARAMPGRDTHIVTLRPDLPYDVYKDMQVLPLHPHNAPLQ
                     VWLELGLPGAILFAALSAGLLLWLARRVPGRMQAAAAASVLSVALAMSSFSYGIWQTW
                     WLAALWLAAVATAAVLRMPARPRG"
     gene            complement(7493..8233)
                     /locus_tag="VGB88_12125"
     CDS             complement(7493..8233)
                     /locus_tag="VGB88_12125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9648241.1"
                     /translation="MPAVPAGLGWRQKAALAAHLFKAVARRHHRALEPVLRQVVPPAG
                     VVLDVGAHAGQFTKLFARLAPAGHVHAIEPGSYARFVLATAVRMLGLGNVTVHALALS
                     DRDGEAELVVPIKAGGGVGFGLGHLAGDEPARKALCERVPVTTLDRFVSRLGLDRLDL
                     IKADVEGAEAALLAGGRDTLARFHPALLVEIDSSRLARAGATPADIWSALAALGYQGR
                     RLDADGRLAERAGDLGDGDYLFRAPAAG"
     gene            complement(8259..10412)
                     /gene="uvrB"
                     /locus_tag="VGB88_12130"
     CDS             complement(8259..10412)
                     /gene="uvrB"
                     /locus_tag="VGB88_12130"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9648242.1"
                     /translation="MAEAAQTIAAPAPGRAAFELVSGFQPAGDQPQAIAELVAGLKRG
                     ERDQVLLGVTGSGKTFTMAHVVRQIARPTLVLAPNKTLAAQLYGEMRSFFPHNAVHYF
                     VSYYDYYQPEAYVPRTDTYIEKDASINEQIDRMRHAATRALLEQDDVVIVASVSCIYG
                     IGSVETYSQMVIDLAAGARIDRNQLLKQLVELQYRRNDANFTRGAFRVRGDGVEIFPA
                     HYEDRAWRLSLFGDEIESIVEFDPLTGDKTDRLDKIRVYANSHYVIPRPTIQQAVKGI
                     RVELKERLAELERQGKLLEAQRLEQRTTFDLEMIETTGSCAGIENYSRYLTGRNPGEP
                     PPTLFEYLPETALLFVDESHVTVPQLGGMFRGDFNRKSTLAEYGFRLPSCVDNRPLKF
                     EEWEAMRPPTVFVSATPGRWEIERTGGAFVEQVIRPTGLIDPVCIVRPAATQVDDLLA
                     ECRTCADRGQRVLVTTLTKRMAEDLTEYLSEAGLRVRYLHSDIDTIERIEIIRDLRLG
                     AFDILVGINLLREGLDIPECALVAILDADKEGFLRSQTSLVQTIGRAARNLDGRCILY
                     ADRMTNSLETAIGETNRRREKQQAYNAANGITPESVRKAIGDILDGVYEADYVTVETG
                     DEAAPHLIGHNLKAYVADLEARMHEHAANLEFEEAARLRDQIRRLEETDLGLTGGRAP
                     GRSTGGRASAMKANRRDDSRRSRGGRHGTARRARR"
     gene            10607..11338
                     /locus_tag="VGB88_12135"
     CDS             10607..11338
                     /locus_tag="VGB88_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648243.1"
                     /translation="MGGTIAVLGVGASQGLGAGIARRFAREGFRAFIAGRNAGRLAQV
                     AAEISDLGGGEATAVPADVTDRDQVVRFFDAVEEAAGVPDVVAYNAGGNRRIPFLELE
                     PETFTNFWRVCCLGGFLVGQEAARRMLPRGAGTITFTGASASRRGRAGFAAFAAGKAG
                     LKMVAESMAREFGPQGLHVAHVVIDGAIDGERIHQRRPDVAAQRGEGGLLAIDDIAEA
                     FWQIHNQGWTAWTFETELRPYKEPF"
     gene            complement(11349..12539)
                     /locus_tag="VGB88_12140"
     CDS             complement(11349..12539)
                     /locus_tag="VGB88_12140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9648244.1"
                     /translation="MGRLGTESAFEVLARAKALEAQGAEVINLGIGQPDFRTPDHIVA
                     AAKKALDDGHHGYTPGNGILPLREAVAADIAKYRGVEVDPARVVIVPGGKVTMWYAIL
                     MFGGPGTEIMYPNPGFPIYESVIRFTGAEPVAIPLSEDKGFAFDAEEVLAKITPRTRL
                     MIVNSPANPTGGVAPRAELDRLVAGLEAHPQVTVLSDEIYARLTYGGAEFVSMLGYEG
                     LRDRLILLDGWSKTYAMTGWRIGYGVWPAPLVDLVTRLNINSVSCTNAATQYAAIAAL
                     AGPQDSVEEMRRAFDARRKVIVELTNDVPGMSSILPAGAFYTMPNITGTGVAAKAMET
                     FLLERLHVAAIAGTSFGAYGEGYIRFSYANSTDNIRTALARIRDFAVAEGWDKLARGA
                     RAAE"
     gene            complement(12655..13839)
                     /locus_tag="VGB88_12145"
     CDS             complement(12655..13839)
                     /locus_tag="VGB88_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648245.1"
                     /translation="MTAGLALSAALAAPGAHALSRAVVLDHGRSLALLTRQVTTRAEV
                     LVTLGELAFKSDFLLEGSPARNIGAHITCDTCHPDGGASRVIFFEGLSDKPGNLDVTN
                     RAISLIEDGIRNPLNVPSIRGAAVTAPFARDGRFESVEDFTLFAIANEFAGGTPSDLV
                     IESLVAYQDALGFADNALLDAAGRLTDSAPEAARRGEAVFARPVPGAAALSCAGCHVP
                     ADNFVDHASHDVGTGRAGRAGKAFDTPTLRDTLITPPYLHDGRYDTLAEVVDYFDGFF
                     ALDLSADERADLTAYLEAVGGGTHAAPAGDGAMHPETAVALLGAALAEDDWLLTRMLV
                     PKLTTELADWRGGAGAPADAVITGWIARLRRIEAQVKSQDFAGARATLDELAAAIAEV
                     AG"
     gene            14003..15205
                     /locus_tag="VGB88_12150"
     CDS             14003..15205
                     /locus_tag="VGB88_12150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9648246.1"
                     /translation="MAFLAERLGRIKPSPTIAVTTRARELRAAGHDVIGLGAGEPDFD
                     TPDPVKEAAIAAIRAGQTKYTAVGGTPELKAAIAAKFKRDNGLDYAPKQIIVGAGGKQ
                     VLFNAILATVEDGDQVIVPAPYWVSYPDMVLLAGGEPVVVETSEDNGFRMRPEDLERA
                     ITPRTKWLILNSPSNPSGAAYSRDELKALAEVLVRRRHVWVMADDMYEKIVYDNFVFT
                     TLAEVEPGLYERTLTVNGVSKAYAMTGWRIGYAGGPEALIQAMDSIQSQSTTNAASIS
                     QAAAVEALNGDQDFIKPRNAAFKKRRDMVVRRLNEVPGLTCRTPEGAFYVYPSCAGVI
                     GKTTPEGKTIATSEDYATHLLESVGVAVVHGTAFGLDPYFRISYATSDAALEEACMRI
                     RRATAALN"
     gene            15223..17280
                     /locus_tag="VGB88_12155"
     CDS             15223..17280
                     /locus_tag="VGB88_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX9648247.1"
                     /translation="MDRDSPLDIAFSACPHDCPSTCALEVERIDTHTIGRVHGARDNS
                     YTAGVVCAKVARYAERVHHPDRLRHPLRRTPSGGFERIPWPDALDEVANAFVRAAQAH
                     GPEAVWPYYYAGTMGLVQRDGINRLRHVMRYSGQHSTICTTLVHSGWKAGVGALIGPD
                     PREMAKADLIVVWGGNPASTQVNAMTHIQRARKERGARLVVVDPYRTHTAQVADQHLA
                     LRPGTDGALACAMMHVLFRDGLADREFMARYADCPERLEQHLSARDPAWAQAITGLDA
                     RVIEDFARRYGTTERSFIRVGYGFSRSRNGAANVHAVSCLPTVTGAWRREGGGAFYNN
                     GAIYHWDKTLIEGLDAVDPAVRMLDQSRIGPVLCGDARDIGDGAPVTAMLVQNTNPAV
                     VAPESTLANQGLARDDLFLCVHEQFMTETAAHADIVLPATTFLEHDDVYQGGGHQHII
                     LGPKVIEPVAEARPNHFVICELAKRLGAKHPGFEMTENEIIDATLRASGWGSLEELRA
                     ARWIDCQPDFEEAHFTAGFPQLDGRFHFAPDWARIGPDHERMPTLPDHHEVIEAADED
                     CPLRMVAAPAHNYLNTSFTETPTSQAKERRPQVKLHPDDARRLGVADGARVRLGNRRG
                     SVVVHAELFDGLQPGVVVVESIWPNKAFEEGRGINTLIGADAGPPNGGAAFHDTAVWL
                     RPA"
     gene            17405..18409
                     /gene="msrP"
                     /locus_tag="VGB88_12160"
     CDS             17405..18409
                     /gene="msrP"
                     /locus_tag="VGB88_12160"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX9648248.1"
                     /translation="MLIKRKPGWALPESAATPEAKFRNRRTIVKGMIAGGLIAGGGVL
                     GARLAGIGEDGGIDAVAAAEDDPNAARYPAERNERLSVDRAVTEERITSTYNNFYEFG
                     SHKNVWKAAQKLAIRPWEVLIDGLVETEVRIQADELIARMPLEERITRHRCVEAWGMT
                     IPWTGFPLARLVELAKPLGSAKYLKMTTFFDPEVAPGHRQTFYPWPYVEGLTMAEATH
                     ELAFIVTGAYGKPLLKQYGTPLRLATPWKYGFKQIKSIVNFSFTEDRPKTFWEIIAPS
                     EYGFWANVNPGFSHPRWSQSSERDLATEERVATKLYNGYGEFVASLYEDMPQDRRLFM
                     "
     gene            complement(18440..19330)
                     /locus_tag="VGB88_12165"
     CDS             complement(18440..19330)
                     /locus_tag="VGB88_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9648249.1"
                     /translation="MDWDKLRVFHVVAESGSFTHAGELLELSQSAVSRQIRALEDSLH
                     VALFHRHARGLIATEQGELLHRITREVFAKVSYAEAMLTESTDRPRGPLRITTTVGFG
                     SMWLTGHVGEFLDNYPEITVSLVVTDAELDLTMRQADVAIRMAPPRQPDLIQRHLFTG
                     RFGVFAIPDYLNRFGTPRTIEDLVEHRVITFGEQVSKPFEDINWLADKVRAFKPDFQP
                     AFTVNNVYGSLRAVETGIGIAGLPAFLARQNPRLVRVLPEIESPKVDAYFVYPEQLRQ
                     SRRVVVFRDFLLRKVAETPF"
     gene            complement(19389..20351)
                     /gene="trxB"
                     /locus_tag="VGB88_12170"
     CDS             complement(19389..20351)
                     /gene="trxB"
                     /locus_tag="VGB88_12170"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9648250.1"
                     /translation="MPDRHHAKVLILGSGPAGLTAAIYAARAGLEPMLVHGLQPGGQM
                     TVTTDVENYPGFAEVVQGPWLMEQMTAQAKAVGTRMFADIAVSVDLARHPFTAQGDSR
                     DTYAGDSLIIATGAQARWLGLDSERKFQGFGVSACATCDGFFFRGREVAVVGGGNTAV
                     EEALFLTNFATKVTLIHRRDSLRADRTNQDRLLRNAKITVLWNHVVEEIVGDEDPPGV
                     TGLKLREVNSGTVSELAVDGVFVAIGHTPASAPFKGQLALDREGYIVTAGDSTATSVP
                     GVFAAGDVQDKVYRQAVTAAGTGCMAALEAERYLAQLDTAEAAE"
     gene            complement(20392..20865)
                     /gene="greA"
                     /locus_tag="VGB88_12175"
     CDS             complement(20392..20865)
                     /gene="greA"
                     /locus_tag="VGB88_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9648251.1"
                     /translation="MQREPMTADGFRRLEAELRKLKTEDRPAVIRAISDARDHGDLAE
                     NAEYHAARERQSFIEGRMRELEDKIGRAQVIDVSKLSGDTVKFGATVTLADEDTDEES
                     TYQIVGVDESDVALGRLSISAPLARALINKAVGDSVGVETPSGLKSFEIVDVKFR"
     gene            complement(20925..24182)
                     /gene="carB"
                     /locus_tag="VGB88_12180"
     CDS             complement(20925..24182)
                     /gene="carB"
                     /locus_tag="VGB88_12180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9648252.1"
                     /translation="MPRREDISSILIIGAGPIVIGQACEFDYSGTQACKALKAEGYRV
                     VLVNSNPATIMTDPGMADATYIEPITPEMVAKIIARERPDALLPTMGGQTALNVAFAL
                     AENGTLARYGIELIGASREAIAKAEDRDLFQAAMANIGLETPRGLVAHAPEEARRALD
                     EIGLPLIIRPSFTLGGTGGGVAFNREEFDQIVAGGLDASPAGEVLIEQSVLGWKEYEM
                     EVIRDRADNCIIVCSIENVDPMGVHTGVSITVAPALTLTDKEYQIMRTASIAVLREIG
                     VETGGSNVQFAVDPDSGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IQNDITGVTPASFEPTIDYVVTKVPRFTFEKFPGTEPLLTTAMKSVGEAMSIGRSFAE
                     SLQKALRSMETGLTGLNEVEIPGYIPGGDRDAILAALARPSPDRVLVIAQALREGLSV
                     ADVNRACRYDPWFLRQIGGIVAAEAAVRANGLPDGARGLAELKRMGFSDARLAELTGR
                     SEAEVAALRARLGVHPVYKRVDTCAAEFDSRTPYMYATYEGDVEAPDAPESEARPSAR
                     DKVIILGGGPNRIGQGIEFDYCCVHAAYALSQAGFETVMINCNPETVSTDYDTSDRLY
                     FEPLTAEDVIGIARAEASAGRLVGLVVQYGGQTPLKLARALEAAGLPIIGTSPDAIDL
                     AEDRERFQGLLTRLGLEQPPNGICRTVEQALEIAERLGYPVLVRPSYVLGGRAMEIVR
                     DRQGLGDYVGAAVRLFGGAPILIDRFLADAVEVDVDALADGEDVHVAGIMEHIEEAGI
                     HSGDSACALPPFSLGAGVIAEIERQTVALAKALGVVGLMNVQFAIKTEAGNAQVYILE
                     VNPRASRTVPFVAKATGVPIAKIAARLMAGEKLKSFGLTARKLAHVSVKEAVFPFARF
                     PGVDVLLGPEMKSTGEVMGIDTDFGRAFAKSQIAAGVPLPDAGNVFISVRDADKKAMV
                     PLGRSLVEMGFTLIATSGTAAFLQAAGLEVRRINKVREGRPHLVDAIIDGQVALIFNT
                     TESGQAIADSYDLRRAALIHKVPYYTTVAGSRAVVQAIKALSRGSLEVAPLQSYT"
BASE COUNT         3464 a         8551 c         8728 g         3440 t
ORIGIN      
        1 cgtcgagccc ggcctcggtc tcgccgcgca gctcgcggat cagcgcccgc cgccgcgccg
       61 acgacaccag cacctcggtc gcgccggcga cgcggtgctg gtaggagtcc ttgcccggat
      121 ggtcgatgtc gaactcgtga tgggcgtgct tgaccgtgga cacgctgagc ccgcgcccga
      181 ccagctcggg caagaggcgg gtgaccagcg tggtcttgcc gctgccgctc cagccggcga
      241 tgccgatcac cttcacggcc ggcccgcggg cgtcaccgcc gacggcccgc cgcccgaagg
      301 ctttgggccg cagggcggtc gcgcgcggcc atgtgcctga ggccgacgcg caggtagtcg
      361 aggccggtga ccgcagtcag cagggcggcg agccacagca gcccgccgcc gagcgcgagc
      421 gcctgatcgg tgccgaggcc gagcgccggc gccgcgccgg cggccagcag cacgcacagg
      481 ctgagcatct ggatcgcggt cttccacttc gccagccggc tgaccggcag cgtcacctcg
      541 cgcccggcca ggaactcgcg caggcccgaa accatgatct cgcggcacag gatgaccagc
      601 gccgccagcg ccggcgcgcg cgcgagccac agcagcatga ccagcgccgc cgcgaccaag
      661 agcttgtcgg cgatcgggtc gaggaaccgg ccgagcgcgg tttcctgctc gcgccggcgc
      721 gccagccagc cgtccgcata gtcggtggcg ctggccgcga cgaacagcac gaaggtcgcc
      781 cagtcggccg cctgcgcggg caggaagaac gcggcgacga ataccggaat cacggccacg
      841 cgcgacaagg tcaggaggtt cggcacgccg agccacatgg cgggggcctg gttagccggc
      901 gccgtggaaa tggtcgtaga tcgcgcgcgc catctgcttg ctcaccccct cgaccttgtc
      961 gagatcggcg agcccggcct cgccgaccgc gcgcgccgag ccgaagcggt tgagcagcgc
     1021 gcgcttgcgc ttgggcccga cgccggcgat ctggtcgagc tcggacaggc cgagcgcgcg
     1081 cgaccggcgc gcgcgatggc cgccgatcgc gaacctgtgc gcctcgtcgc gcaggcgttg
     1141 caggaagtag ctcgccgggg tgttgggcgg cagcatgaac ggcgcccgtc ccggcaggtg
     1201 gatgcgctcg cgcccggcgt cacggtccgg ccccttggcg atcgcggcca ccgtcaggtt
     1261 gccggggccg acctcgatgc cgaggtcggc gaacacctcg agcgcgctcg acagctggcc
     1321 ggcgccgccg tcgagcagca ccaggtcggg ccactggccc tgggcgcgct ccgggtcctc
     1381 cttctgcagg cgggtgaagc ggcggcgcag cacctcgcgc atcatcgcgt agtcgtcgcc
     1441 gggggcgaag ccggcgccgt cgccgtcgcg cgcgccgcgg atgttgaact tgcgatagcc
     1501 gcccttgacc aagccctgcg ggccggccgc gatcattgcg ccgaccgcgc gcgtgcccga
     1561 gatgtggctg ttgtcataga cctcgatccg ctcgggcgcg gtctccatcg ccaggagctg
     1621 ggcgatgccg tcgagctggc ggcgctgcga ggcgctttcg gccagccgcc gggccagcgc
     1681 ctcgcgcgca ttggcgaccg cgttgtcgac cagctggcgc ttgtcgccgc gctgcgggca
     1741 caggatgcgc acccgccgct cggcgcgcag cccgagcgcc tcctcgatca gcgcctggcc
     1801 gtcgagcgcg tcgctggcca ggatcagcgg cggcggcgtg cgcgagtcgt agaactgggc
     1861 gacgaaggcg gcgagcactc ccgccaccgg ctcgtcgcgc gcgtggctcg ggaaatgggc
     1921 ccggttgccg tagttgcggc cgctgcggaa gaagaacacc tggacgcagg tctgcccggc
     1981 ctgctggtcg gcggcgatca cgtcggcgtc gccgatcgcc tcgacgttga tcccttggtg
     2041 cgcctggata tgggcgagcg cgcggatgcg gtcgcgcagc accgccgcgc cctcgaactc
     2101 gagcgcgtcg ctcgcctttt gcatgctctc ggcgagacgg tcctggaccg cctggctgcg
     2161 cccggacagg aagtcgcgcg tctggtcgac gatctcggcg tagtcctcgg ccgcgatcag
     2221 gccgacgcac ggcgcggtgc agcgcttgat ctgatattgc aggcaggggc gggtgcggcc
     2281 cgcgaacacg ttgtccgagc aggtgcgcag cgggaacgcg cgttgcagcg cattgagcgt
     2341 gcggttgacc gcgccggccg aggcaaaggg gccgaagtag cgcccggggc ggctgcgcgc
     2401 gccgcggtgc ttgagcagct gcggccagtc gctgtcgccg gtcagcagga tgtaggggtg
     2461 cgacttgtcg tcgcgcagca cgatgttgta gtgcggcttg aggcgcttga tcatgttcgc
     2521 ctcgagcagc agcgcctcgg cctcggtgtg ggtggtcacc acctcgaccg cggcggtctc
     2581 gccgatcatg cgcgcgatgc gggtcgactg gcggatcggg ttggcgtagg cggcgacgcg
     2641 cttgcgcagg ctgcgcgcct tgccgatata gagcgccttg ccgtgggcat tgagcatgcg
     2701 atagacgccg ggccccggcg gcagcgccgc aagggcgtcg cgaatcaccg ccgcgccgcg
     2761 gcggcgcgag gcctccggcc gcggtcgctc ggctgcggcg ccggctttgt ccgagacggc
     2821 catggccgca atatcgccac gccgcgggcc gcggtcaacg cgccccatga cggcgccggg
     2881 cgcggggacg ccggcctttc cggggatcac tcccgctatc cacggaacct gtggaaaacc
     2941 cggtgaaaaa cctgcgcgat gagccgccgg cacgcccgcc cgcgggcccg cgcggctctc
     3001 gacgcgcgaa cccggaaatc tattaagaga ttatcatcaa aggatatttt gttgccgggg
     3061 cacgatcacc ggtgagtctc gaaaggaata ccgcaaccac ggcgcgcaag acctgccgcc
     3121 ggggagcgct gtgaacaaaa tcatgtgacc ggattgtgac gaaggctctg aaaacgcttc
     3181 gttattcggc caggcgacgc tcgatctcga cacccgcccc ggcggcgtcg gggagcacat
     3241 cgagcttctc gatgcgtacc cgcaccacct gcacgcgccg gtcggcgagg cacaggtcgg
     3301 cgatccgctc ggccagcgtc tcgaccaggt tgatgtggcc ctcgtcgacc acgcggcgga
     3361 cgccctgggc gatgtcgtcg tagctgacca ccgccgccag gcgatcgtcg tgcggcgcgt
     3421 cgggctcggc catctccagc tcgacgccga tgcgcacccg ctggcgcttg ccgcgctcat
     3481 gggcgtaggc gccgatcgca cagcgcagct cgaggtcgcg gataaacagc cgccggcgca
     3541 cggtgcgcgg caccggctgg gggatggggt ggaccttgcc cggcggggtc atcgcgtatc
     3601 ctactccggc tcgggcgcgc ccggctcgcc cgggtgctgc cagccgagat gctggccgcc
     3661 gtcgagggcg atcatttgcc cggtcagcga cggcgcgtcc acgacaaagc ggagcgcggc
     3721 gcagatctcg ttgaggccga cctggcggcg gagcggggtg cgcgcgacct gggcggcgaa
     3781 cgactccgcg ctctggcgcg cgctcgccag caccggcccg gggccgatgg cattgacccg
     3841 gatgcgcggc gccagcgcca gcgccagcgt gcgcgtcaag gtccacagcc cggccttgct
     3901 cagcgtgtac gagacgaagt gcgggctcag gttccacacc cgctggtcga tcatgttgac
     3961 gatcgtcccg ctcaggccct cgggcagggc gcgcgcgaac gcctgcgaca gcacgaacgg
     4021 cgcgcgcagg ttggtgtcga gatgcaggtc ccagctccgc cgggtcgcgg tcgcgaccga
     4081 gtcgtactcg aacgcggccg cgttgttgac caggcaggtg accgcgccgg cggcggccgc
     4141 ggctgcggcg tcgaccagcc ccgcggcctc ggcctcgacc gcgaggtcgg cggcgagcgc
     4201 gaccgcgtgc ccgccgccgg cgacgattcg atcgaccacc tcggcagcgg cgtcgcccga
     4261 gcggcgatga tgcaccgcca ccgcccagcc gtgggcggcg aggtcgagcg ccagcgcgcg
     4321 cccgatccgc gtgccggcgc cggtcaccag cgcgagtccg cggcggtcag cggtcatgcg
     4381 cgcacgatgg cgaaacttcg ccgccggcgc aacgccggcc gtgcgcccat gccgggcttg
     4441 accgtaaccc gccgccgcct gctaatcgcc gatatggagt cgcagttcct gccgcaggtg
     4501 ctggtccttc tgatcgccgc cgtggtgatc gtgttcgcgt tcgagcggct ccggctcagc
     4561 cccgtgctcg gctatatcgt cgccggcgcg gcgatcgggc cgtacgggct gaagctgatc
     4621 tcgggcgtcg aggaaacgcg ggcgctggcc gagctcggca tcgtcttcct gctgttcatg
     4681 atcgggctcg agctgccgta cgagcggctc gccgcgatgc gccgctacgt gctcgggctc
     4741 ggcgtgctcc aggtcgcgat caccggggtc atcttcggcg gccttgcccg ctggctcgga
     4801 caaagcctcg aatcgtccgt ggtgatcggc gtcgggctgg cgctgtcctc gaccgcgatc
     4861 gcgctccagc tgctggtgct gcgcggcgag cagaccaccc cgctcggccg ggcctcgatc
     4921 gcgatcctga tcacccagga cctgctggtg gtgccgatgc tggtgctgat cccgggcttg
     4981 ggcgacgagg ccggctcgct caccggcgca ctcggcgagg cgctgctgca ggcggcggtc
     5041 gtgctggccg cgatcatcct tgccgggcga ttgttgctgc ggcacgtatt ccgcctcatc
     5101 gcccgggcgc ggcaggccga gctgctgacc gccaccgcgc tggtggtgat cctgggcacc
     5161 gcggcggtca ccgcgcaagt cgggctgtcg ctggcgctcg gcgcgttcat cgccggtctg
     5221 ctgctcgccg agtccgagta ccgccaccac gtcgaggccg acatcgcccc gttccgcggc
     5281 atcctgctcg gcctgttctt catgaccgtc ggcatgtcgg tcgacatcgg ccggctgccg
     5341 gccgagctca gcaccgtggc ggccctggtc gcggtcctgc tcgcggtcaa ggcggcggtg
     5401 ctgttcgcgc tggtcagctt gacccggctc ggcgccgccg ccgcggtgcg caccgcgctg
     5461 ctgctgtgcc agggcggcga gttcgcgttc atcatgttca cccttgcgat cgagcacggg
     5521 gtgatggcgc aggcgaccta cgaccttttg atcctggtgg tgggcgtgac catggccatg
     5581 acccccgggc tcgcctggat cggccgggcg ctggcgcggc acatgatggc gggagaggcg
     5641 gaccggcatg gcgggctggc ggcgggggcg gcggagctcg aaggccatgt cgtcatcctc
     5701 ggcttcggcc gggtcggcca gacgatcgcg accatgctca aggcgatcga catcccctat
     5761 gtcgcggtcg atctcgagcc gcggcgggtg atcgagggcc gcgcccgcgg cctgccggtg
     5821 ttctacgccg acgcgagccg cccccaggtg ctcaggcgcg ccaatgtcga gcgcgcctgc
     5881 gcggcggtgg tcgcggtcga caacccggcg aacgcggtgc gcgtggtcga cgcgatccgg
     5941 cgcgcgggcc ccgaggtcgg catctatgcg cgtgcccgcg accgcaccga gatgcagcgc
     6001 ctggaggccg ccggcgccac ggtggtaatc cccgacacgc tcgaggcgag cctgcagctc
     6061 ggcggccagc tcctgtcgcg ggtcggcatg tccgacaccg acatcgaccg gctgtgcgcg
     6121 gagctgcgca ccgaaggcta cgggcgtctc gccgacatga tcccggcgcg cacgccgccc
     6181 gccgccgccg aacgcaccgc cgacgcgccc cgcgccgccg agtgagccgc gcccgccacg
     6241 ttcagccgcg cggccgcgcc ggcatgcgca gcaccgcggc ggtcgccacc gccgccagcc
     6301 acagcgccgc cagccaccag gtctgccaga tgccgtagct gaagctcgac atcgccagcg
     6361 ccacgctcag cacgctcgcc gccgccgccg cctgcatgcg gcccggcacg cggcgcgcga
     6421 gccacagcag caatcccgcc gacaacgccg cgaacaggat cgcgcccggc aggccgagct
     6481 cgagccagac ctgcagcggc gcattgtgcg ggtgcagcgg cagcacctgc atgtccttgt
     6541 agacgtcgta gggcaggtcg gggcgcaggg tgacgatgtg ggtgtcgcgc ccgggcatcg
     6601 cgcgcgccgc atcgagcccc cagccgagcc acggccgctc gaggatgcgc tcggcggtga
     6661 agtgccagat gaacaggcgg tggaccgcgg tcgagccgag ccggtcggtc caggcgagca
     6721 cccggtcggg ggacaggatt gtcagcgaca gcgccggcgc ggccatggtg ccgagcgcga
     6781 gcgccgcggc cagcaggcgc gtgccgatgc cgcgcgccag cgcgacgacg gcgaaggtcg
     6841 cggcgccgac cgcgagcgcg gccatcgagg actcgctttc gagcaaggcc agcaagccga
     6901 cgcagctcaa cgccagcgcc ggcgccgcgg cgcgcatcca gcgccggtcg gcgcgcacgc
     6961 cgcgccgcca cagccacagc gccgccggcc acaccacgag caccgcgacg gtcgcgccgc
     7021 ggttgaggct ggacatgctg tagtagcccg ggtcgtcgcg gatcgcgtgc ctcagcgcgc
     7081 cgtcgagcat ggcgtcggcg gccatgaatg cgaccccgag cgcgagcccc gcgcacaggc
     7141 cggcgccgag ccatgactgg cggtccgccg gcagcgccag ggtcccggcc aggatcagcg
     7201 gcccgaccac ggcgatcaag gtgagcacct ccgcccggcg gaccgcgagg cccgggtcga
     7261 gcgcccacag cgcgctcagc cagcaccagg cgagcagcgc cgcgatcgcg gcggtgagcc
     7321 acaggaacgc cggcggcaac ggctgcgcgc gcgcgcgcag cgcggcgatc gcgaacagcc
     7381 cggcgacgac gccgagcggc gtcagcccct tcggcgccaa tgccgccagc ggcaccacca
     7441 ggaacgcggt cgcggccagc ccccacgccg cgacggagcc gagcctggtc attcagccgg
     7501 ccgccggcgc gcggaacagg tagtcgccgt cgccgaggtc gccggcccgc tcggcgagcc
     7561 tgccgtcggc gtcgaggcgc cggccctggt agccgagcgc ggcgagcgcc gaccagatgt
     7621 cggcgggcgt ggcgcctgcg cgcgcgagcc ggctcgagtc gatctcgacc agcagtgccg
     7681 ggtggaaccg ggcaagcgtg tcccgcccgc cggcgagcag cgcggcctcg gccccctcga
     7741 cgtcggcctt gatcaggtcg agccggtcga ggccgaggcg cgaaacgaat cgatcgaggg
     7801 tggtcaccgg cacgcgctcg cacagcgcct tgcgcgccgg ctcgtcgccg gccaggtggc
     7861 cgaggccgaa gccgaccccg ccgccggcct tgatcggcac caccagctcg gcctcgccgt
     7921 cgcggtcgga caaggcgagc gcatggacgg tgacgttgcc gagaccgagc atgcggaccg
     7981 cggtcgcgag cacgaagcgg gcatagctgc cgggctcgat cgcgtgcaca tgcccggccg
     8041 gcgccaggcg cgcgaacagc ttggtgaact ggccggcatg ggcgccgacg tcgagcacca
     8101 cgcccgccgg cggcaccact tgcctcagca ccggttcgag cgcgcgatgg tggcggcgcg
     8161 ccaccgcctt gaacaggtgc gcggcgagcg ccgccttctg gcgccagccg aggccggcgg
     8221 ggactgcggg catcgcggag cctgcgccgg ccgacgcctc agcggcgtgc gcgccgcgcg
     8281 gtcccgtgcc ggccgccgcg gctgcgccgc gaatcgtcgc gccggttcgc cttcatggcg
     8341 ctggcccgcc cgccggtcga gcgtcccggc gcccgcccgc ccgtgagccc gaggtcggtc
     8401 tcctcgagcc gcctgatctg gtcgcgcagc cgcgccgcct cctcgaactc gaggttggcg
     8461 gcgtgctcgt gcatgcgcgc ttccagatcg gcgacgtagg ccttgaggtt gtggccgatc
     8521 aggtgcggcg ccgcctcgtc gccggtctcg acggtgacgt agtccgcctc gtagacgccg
     8581 tccaggatgt cgccgatcgc cttgcgcacc gattcgggcg tgatgccgtt ggcggcgttg
     8641 taggcctgct gcttctcgcg ccggcggttg gtctcgccga tcgcggtctc gagcgagttg
     8701 gtcatgcggt cggcgtagag gatgcagcgg ccgtcgaggt tgcgcgcggc gcggccgatg
     8761 gtctggacca gcgaggtctg cgagcgcagg aagccctcct tgtcggcgtc aaggatcgcg
     8821 accagcgcgc attctgggat gtcgagcccc tcgcgcagca ggttgatgcc gaccaggatg
     8881 tcgaacgcgc cgaggcgcag gtcgcggatg atctcgatcc gctcgatggt gtcgatgtcg
     8941 gagtgcaggt agcgcacgcg caggccggcc tcgctcaaat attcggtcag gtcctcggcc
     9001 atgcgcttgg tcaaggtggt caccagcacc cgctggccgc ggtcggcgca ggtgcggcac
     9061 tcggccagca ggtcgtcgac ctgggtcgcg gcggggcgga cgatgcacac cgggtcgatc
     9121 agcccggtcg ggcggatcac ctgctcgacg aacgcgccgc cggtgcgctc gatctcccag
     9181 cgcccgggtg tcgccgagac gaacacggtc ggcgggcgca tcgcctccca ctcctcgaac
     9241 ttgagcgggc ggttgtcgac gcacgacggc agccggaagc cgtactcggc gagggtggac
     9301 ttgcggttga agtcgccgcg gaacatgccg ccgagctggg gcacggtgac atggctctcg
     9361 tcgacgaaca ggagcgcggt ctcgggcagg tactcgaaca gcgtcggcgg cggctcgccg
     9421 gggttgcgcc cggtgagata gcgcgagtag ttctcgatgc ccgcgcacga gcccgtggtc
     9481 tcgatcatct cgaggtcgaa ggtggtgcgc tgctcgagcc gctgcgcctc caagagcttg
     9541 ccctggcgct cgagctcggc gagccgctcc ttgagctcga cgcggatgcc cttgaccgcc
     9601 tgctggatgg tcgggcgcgg gatcacgtag tggctgttgg catagacgcg gatcttgtcc
     9661 agccggtcgg tcttgtcgcc ggtcaaagga tcgaactcga cgatcgactc gatctcgtcg
     9721 ccgaacaagg acagccgcca ggcgcggtcc tcgtaatggg ccgggaagat ctcgaccccg
     9781 tcgccgcgca cccggaacgc gccgcgggtg aagttggcgt cgttgcgccg gtactgcagc
     9841 tcgaccagct gcttgagcag ctggttgcgg tcgatgcgcg cgccggcggc gaggtcgatg
     9901 accatctgcg agtaggtctc gaccgagccg atgccgtaga tgcatgacac gctggcgacg
     9961 atcaccacgt cgtcctgctc cagcagcgcg cgcgtcgccg cgtgccgcat gcggtcgatc
    10021 tgctcgttga tcgaggcgtc cttctcgata taggtgtcgg tgcgcggcac atatgcctcg
    10081 ggctggtagt agtcgtagta cgagacgaag tagtgcaccg cgttgtgcgg gaagaacgac
    10141 ctcatctcgc cgtagagctg ggcggcgagc gtcttgttgg gcgcgagcac cagggtcggg
    10201 cgcgcgatct ggcgcaccac gtgcgccatg gtgaaggtct tgcccgagcc ggtgaccccg
    10261 aggaggacct ggtcgcgctc gccgcgcttg agccccgcga ccagctctgc gatcgcctgc
    10321 ggctggtcgc ccgcgggctg gaacccggag accagctcga acgccgcgcg cccgggcgcc
    10381 ggcgccgcga tcgtctgtgc cgcttccgcc atggccgcaa atatcggcga gggggatggc
    10441 ggatgcaatg cgcccgtttc gatgaaccac agaggacaca gaggaattgt tattcggaaa
    10501 aaagcgcggc ccgcgcgggc cgcttgcgga aaataccttt ctctgtgaac tctatgtcct
    10561 ctgtggttcc ctttcttgac cgcgcgcgag cgcgaaggag cctgacatgg gcggcacgat
    10621 cgcggttctc ggcgtcggcg cgagccaggg cctcggcgcc ggcatcgcgc gccggttcgc
    10681 gcgcgagggc ttccgcgcct tcatcgccgg tcgcaacgcc ggccgcctcg cccaggtcgc
    10741 ggccgagatc agcgacctcg gcggcggcga ggcgaccgcg gtgccggccg acgtcaccga
    10801 ccgcgaccag gtggtgcgat tcttcgatgc ggtcgaggag gcggcgggcg tgcccgacgt
    10861 ggtcgcctac aacgccggcg gcaaccggcg catcccgttc ctcgagctcg agcccgagac
    10921 cttcaccaac ttctggcggg tgtgctgcct gggcggcttc ctggtcggcc aggaggcggc
    10981 gcggcgcatg ctgccgcggg gggcgggcac gatcaccttc accggcgcct cggccagccg
    11041 gcgcgggcgc gccggcttcg ccgccttcgc cgccggcaag gccgggctca agatggtcgc
    11101 cgagtcgatg gcgcgcgagt tcgggccgca gggcctccac gtcgcccatg tggtgatcga
    11161 cggcgccatc gacggcgagc gcatccacca gcgccgcccc gacgtcgccg cccagcgcgg
    11221 cgagggcggc ttgttggcca tcgatgacat cgccgaggcg ttctggcaaa tccacaacca
    11281 gggctggacc gcctggacct tcgagaccga gctccgcccc tacaaggagc cgttctgagg
    11341 cgcggacgct actcggcggc gcgggcgccg cgcgccagct tgtcccagcc ctcggcgacg
    11401 gcgaagtcgc ggatgcgggc gagcgcggtg cggatgttgt cggtcgagtt ggcgtaggag
    11461 aagcggatgt agccctcgcc gtaggcgccg aagctggtgc cggcgatcgc ggcgacatgc
    11521 aggcgttcca gcaggaaggt ctccatcgct ttcgccgcca cgccggtgcc ggtgatgttg
    11581 ggcatggtgt agaacgcgcc cgcgggcagg atcgagctca tccccggcac gtcgttggtc
    11641 agctcgacga tcaccttgcg gcgggcgtcg aacgcgcgcc gcatctcctc gaccgagtcc
    11701 tgcgggcccg cgagcgcggc gatcgcggcg tactgggtgg cggcgttggt gcacgagacc
    11761 gagttgatgt tgagccgggt gaccaggtca acgagcggcg ccggccacac gccgtagccg
    11821 atccgccagc cggtcatggc gtaggtcttg ctccagccgt cgagcaggat cagccggtcg
    11881 cgcaagccct cgtagccgag catggagacg aactcggcgc ctccataggt gagccgggcg
    11941 taaatctcgt ccgacaatac ggtcacctgc gggtgcgcct cgagcccggc gaccaggcgg
    12001 tcgagctcgg cgcgcggcgc caccccgccg gtcgggttgg cggggctgtt gacgatcatc
    12061 agccgggtgc gcggcgtgat cttggccagc acctcctcgg catcgaaggc gaagcccttg
    12121 tcctcgctga gcgggatcgc caccggctcg gcgccggtga acctgatcac cgactcgtaa
    12181 atcgggaagc ccgggttggg gtacatgatc tcggtgccgg ggccgccgaa catcaggatc
    12241 gcgtaccaca tggtgacctt gccgccgggc acgatcacca cccgggcggg atcgacctcg
    12301 accccgcggt acttggcgat gtcggcggcg acagcctcgc gcaggggcag gatgccgttg
    12361 cccggggtgt agccgtggtg gccgtcgtcg agcgccttct tggccgcggc gacgatgtgg
    12421 tcgggggtgc ggaagtcggg ctggccgatg ccgaggttga tcacctcggc gccttgggcc
    12481 tcgagcgcct tggcgcgggc gagcacctcg aacgcgctct ccgtgcccag ccggcccatg
    12541 ctgtcgttga gctcgaatcc ccctagccgc accgtatcct cccgtgtctt gagccggccg
    12601 gcggccggca tcggagtatg cgccaccgcc gactgtcgcg gcaacggcgc cggcctaacc
    12661 ggcgacctcg gcgatcgctg cggcgagctc gtcgagggtg gcgcgcgcgc cggcgaagtc
    12721 ctgggacttg acctgggcct cgatgcgcct gaggcgcgcg atccagccgg tgatcaccgc
    12781 gtcggcgggc gcgcccgcgc cgccccgcca gtcggcgagc tcggtggtga gcttgggcac
    12841 cagcatccgg gtcagcagcc agtcgtcctc ggcgagcgcg gcgccgagca gtgccaccgc
    12901 cgtctcgggg tgcatggcgc cgtcgccggc gggtgcggcg tgggtgccgc cgccgaccgc
    12961 ctccagatag gcggtgaggt cggcgcgctc gtcggcactc aggtcaagcg cgaaaaagcc
    13021 gtcgaagtag tcgaccacct cggcgagcgt gtcgtagcgg ccgtcgtgca gatagggcgg
    13081 ggtgatcagc gtgtcgcgca aggtcggcgt atcgaacgcc ttgccggcgc gcccggcgcg
    13141 gccggtgccg acatcgtgac tggcatggtc gacgaagttg tccgcgggca cgtggcagcc
    13201 ggcgcagctc agcgccgccg cgcccgggac ggggcgcgcg aacaccgcct cgccgcgccg
    13261 cgcggcctcc ggcgccgagt cggtcagccg gcccgccgca tccagcagcg cattgtcggc
    13321 gaagccgagc gcgtcctggt aggcgaccag cgactcgatc accaggtccg acggcgtgcc
    13381 gccggcgaac tcgttggcga tcgcgaacag ggtgaagtcc tcgaccgact cgaagcggcc
    13441 gtcgcgcgcg aacggcgcgg tcacggcggc gccgcggatg ctcggcacgt tgagcgggtt
    13501 gcggatgccg tcctcgatca gcgagatcgc gcggttggtg acgtcgagat tgccgggctt
    13561 gtcggacagg ccctcgaaga agatcacccg gctcgcgccg ccgtcggggt ggcaggtgtc
    13621 gcaggtgatg tgcgcgccga tgttgcgcgc cggcgagccc tcgagcagga agtccgactt
    13681 gaaggcgagc tcgccgaggg tcaccagcac ctcggcgcgc gtcgtcacct ggcgggtgag
    13741 caaggccagg ctccggccgt ggtcgaggac gacggcgcgt gacagggcgt gcgcccccgg
    13801 cgccgccagg gcggccgaca gggcaaggcc ggcagtcacg gcaagcgcga ctcgcgcgcg
    13861 ggttgttttc ggcgccttcg gcatggccgc aggaatctag gagagcggcg acccaccggc
    13921 aagcgccggc gcttgtcagg caggcggcct gcgcgtagct tgcgcgggcg aaccaacccg
    13981 gccaaccgga cccgaaacga tcatggcctt cctcgccgaa cgcctggggc ggatcaagcc
    14041 ctcgccgacc atcgcagtca ccacgcgcgc gcgcgagctg cgcgcggccg gccacgacgt
    14101 gatcgggctt ggcgcgggcg agcccgactt cgacaccccc gacccggtca aggaggcggc
    14161 gatcgcggcc atccgcgccg gccagaccaa gtataccgcg gtcggcggca cgcccgagct
    14221 gaaggcggcc atcgccgcca agttcaagcg cgacaacggc ctcgactacg cgcccaagca
    14281 gatcatcgtc ggcgccggcg gcaagcaggt gctgttcaac gccatcctgg ccaccgtcga
    14341 ggacggcgac caggtgatcg tgccggcgcc gtactgggtg tcgtacccgg acatggtgct
    14401 gcttgcgggc ggcgagccgg tggtggtgga gaccagcgaa gacaacggct tccgcatgcg
    14461 gcccgaggac ctggagcggg cgatcacgcc gcgcaccaag tggctgatcc tcaactcgcc
    14521 gtcgaacccc tcgggcgcgg cctactcgcg cgacgagctc aaggcgctgg ccgaggtgct
    14581 ggtgcgccgc cgccacgtct gggtgatggc cgacgacatg tacgagaaga tcgtctacga
    14641 caacttcgtg ttcaccacgc tggccgaggt cgagcccggc ctgtacgagc gcacgctcac
    14701 cgtcaacggc gtgtccaagg cctacgccat gaccggctgg cggatcggct atgccggcgg
    14761 gccggaggcg ttgattcagg cgatggacag catccagtcg cagagcacga ccaatgccgc
    14821 ctcgatcagc caggcggcgg cggtcgaggc gctcaacggc gaccaggact tcatcaagcc
    14881 gcgcaacgcc gcgttcaaga agcgccgcga catggtggtg cgccggctca acgaggtgcc
    14941 cgggctcacc tgccgcacgc cggaaggggc gttctacgtc tatccgagct gcgccggggt
    15001 gatcggcaag accacgcccg agggcaagac catcgccacc agcgaggact acgccacgca
    15061 cctgttggag agcgtgggcg tcgcggtcgt ccacggcacc gcgttcggcc tcgaccccta
    15121 tttccgcatc tcctatgcta cctcggacgc ggcgctcgag gaggcgtgca tgcgcatccg
    15181 gcgcgccacc gccgcactca actagcccgg acggacgccg tcatggaccg cgactcgccg
    15241 ctcgatatcg ccttctcggc ctgcccgcac gactgcccga gcacctgcgc gctcgaggtc
    15301 gagcgcatcg atacccacac catcggccgg gtccacggcg cgcgcgacaa cagctacacc
    15361 gcgggcgtgg tgtgcgccaa ggtcgcgcgc tacgccgagc gcgtccacca ccccgaccgg
    15421 ctgcgccacc cgttgcggcg cacgccctcg ggcggcttcg agcgcatccc gtggccggac
    15481 gcgctcgacg aggtcgccaa cgccttcgtc cgcgcggccc aggcgcatgg gcctgaggcg
    15541 gtgtggccct actactacgc cggcaccatg ggcctggtgc agcgcgacgg catcaaccgg
    15601 ctgcgccacg tgatgcgcta ctcgggccag cactcgacga tttgtaccac cctggtccat
    15661 tccggctgga aagcgggcgt cggcgcgctg atcggccccg acccgcgcga gatggcgaag
    15721 gccgacctga tcgtggtctg gggtggcaac ccggcctcga cccaggtcaa cgccatgacc
    15781 cacatccagc gcgcgcgcaa ggagcgcggc gccaggctgg tcgtggtcga cccctaccgc
    15841 acccacaccg cccaggtcgc cgaccagcac ctggcgctgc gaccgggcac cgacggcgcg
    15901 ctcgcctgcg cgatgatgca cgtgctgttc cgcgacggcc tcgccgaccg cgagttcatg
    15961 gcgcgctacg ccgactgccc ggagcggctc gagcagcacc tgtcggcgcg cgacccggcc
    16021 tgggcgcagg cgatcacggg gctggatgcg cgcgtgatcg aggacttcgc gcgccgctac
    16081 ggcaccaccg agcgcagctt catccgcgtc ggctacggct tctcgcgctc gcgcaacggc
    16141 gccgccaacg tccatgcggt gagctgcctg ccgaccgtga ccggcgcctg gcgccgcgag
    16201 ggcggcggcg cgttctacaa caacggcgcc atctaccact gggacaagac cctgatcgag
    16261 gggctggacg cggtcgaccc cgcggtgcgc atgctcgacc agtcgcgcat cgggccggtg
    16321 ctgtgcgggg acgcgcgcga catcggcgac ggtgcgccgg tgaccgcgat gctggtccag
    16381 aacaccaacc cggcggtggt cgcgcccgaa tcgacgctcg ccaaccaggg cctcgcgcgc
    16441 gacgacctgt tcctgtgcgt gcacgagcag ttcatgaccg agaccgcggc ccatgccgac
    16501 atcgtgctgc cggcgaccac gttcctggag catgacgacg tctaccaggg cggcggccac
    16561 cagcacatca tcctcgggcc gaaagtgatc gagccggtgg ccgaggcgcg gcccaaccac
    16621 ttcgtcatct gtgagctggc caagcgtttg ggcgccaagc atcccggctt cgagatgacc
    16681 gagaacgaga tcatcgacgc caccctgcgc gcatcgggct gggggtcgct cgaggagctg
    16741 cgcgcggcgc gctggatcga ctgccagccg gacttcgagg aggcgcattt cacggccggc
    16801 ttcccgcagc tcgacggccg cttccatttc gcccccgact gggcgcgcat cggccccgac
    16861 cacgagcgca tgccgacgct gcccgaccac cacgaggtca tcgaggcggc ggacgaggac
    16921 tgcccgttgc gcatggtcgc ggcgccggcg cacaactatc tcaacaccag cttcaccgag
    16981 accccgacct cgcaggcgaa ggagcgccgc ccgcaggtca agctccatcc cgacgacgcc
    17041 cgccggctcg gcgtcgccga cggcgcgcgc gtcaggctcg gcaaccggcg gggcagcgtg
    17101 gtcgtccatg ccgagctgtt cgacggcctg cagccgggcg tggtggtggt cgagagcatc
    17161 tggccgaaca aggcgttcga ggaagggcgc ggcatcaaca ccctgatcgg ggccgatgcg
    17221 ggcccgccca acggcggcgc ggcgttccac gacaccgccg tctggctgcg cccggcgtaa
    17281 ggcgcggcgc gcgcacacgc cccttcaaca tcgcgtgagc gccggtagcc gggactggcg
    17341 ccgcgcccga tctccgggtc acgatgccaa gggccggagc cggccgatcg agtggagaga
    17401 cgacatgctg atcaagcgca agcccggatg ggcgctgccc gagagcgcgg caacgccgga
    17461 agcgaagttc cgcaaccgcc gcaccatcgt caagggcatg atcgccggcg gcctgatcgc
    17521 cggcggcggc gtcttgggcg cgcggctcgc cggcatcggc gaggacggcg gcatcgacgc
    17581 cgtggccgcg gccgaggacg accccaacgc cgcccgctac ccggccgagc ggaacgagcg
    17641 cctgtccgtg gaccgggccg tgaccgagga gcggatcacc tccacctaca acaacttcta
    17701 cgagttcggc tcgcacaaga acgtgtggaa ggcggcgcag aagctcgcca tccgcccgtg
    17761 ggaggtgctg atcgacggcc tggtcgagac cgaggtccgc atccaggcgg acgagctgat
    17821 cgcgcgcatg ccgctcgagg agcgcatcac ccgccaccgc tgcgtcgagg cgtgggggat
    17881 gaccattccc tggaccggct tcccgctcgc caggctggtc gagctggcca agccgctggg
    17941 ctcggcgaag tacctcaaga tgaccacctt cttcgacccc gaggtggcgc cgggccaccg
    18001 ccagaccttc tatccgtggc cctatgtcga gggcctgacc atggccgagg cgacgcatga
    18061 gctcgccttc atcgtcaccg gcgcctacgg caagccgctg ctgaagcagt acggcacccc
    18121 gttgcgcctg gcgacgccgt ggaagtacgg cttcaagcag atcaagtcga tcgtcaactt
    18181 cagcttcacc gaggaccggc cgaagacctt ctgggagatc atcgcgccga gcgagtacgg
    18241 cttctgggcg aacgtcaacc cgggcttctc gcacccgcgc tggagccagt cgagcgagcg
    18301 cgacctggcc accgaggagc gggtcgcgac caagctgtac aacggctacg gcgagttcgt
    18361 ggcgtcgctc tacgaggaca tgccgcagga ccgcaggctg ttcatgtagc ggcgtggcgc
    18421 cggcgcgcga gccggctggt cagaacggcg tctcggcgac cttgcgcagc aggaagtcgc
    18481 ggaacaccac cacccgccgg ctctggcgga gctgctcggg atagacgaag taggcgtcga
    18541 ccttgggcga ctcgatctcg ggcagcaccc gcaccaggcg cgggttctgg cgggcgagga
    18601 acgccggcag cccggcgatg ccgatgccgg tctccaccgc gcgcaggctg ccatagacgt
    18661 tgttgaccgt gaacgcgggc tggaagtcgg gcttgaacgc gcgcaccttg tcggccagcc
    18721 agttgatgtc ctcgaacggc ttcgacacct gctcgccgaa ggtgatgacg cggtgctcga
    18781 ccaggtcctc gatggtgcgc ggcgtgccga agcggttgag atagtcggga atcgcgaaca
    18841 cgccgaagcg gccggtgaac aggtggcgct ggatcaggtc gggctggcgc ggcggcgcca
    18901 tgcggatcgc cacgtccgcc tggcgcatgg tcaggtcgag ctcggcgtcg gtgaccacca
    18961 ggctgaccgt gatctcggga tagttgtcca ggaactcgcc gacgtggccg gtcagccaca
    19021 tcgagccgaa gccgaccgtg gtggtgatcc tgagcgggcc gcgcgggcga tcggtgctct
    19081 cggtcagcat cgcctcggcg tacgagacct tggcgaacac ctcgcgggtg atgcggtgca
    19141 gaagctcgcc ctgctcggtc gcgatcaggc cgcgcgcatg gcggtggaac agggccacgt
    19201 gcagcgagtc ctcgagcgcg cgaatctggc ggctgaccgc cgactggctg agctcgagca
    19261 gctcgccggc atgggtgaag ctgcccgact cggcgaccac gtggaacacg cgcagcttgt
    19321 cccaatccat ggccgcgact ctactgggcg ttgccgtgcc gcgcgagcgc gaatccccgc
    19381 gtcggccgct actcggcggc ctcggcggtg tcgagctggg cgagatagcg ctcggcctcg
    19441 agcgcggcca tgcagccggt gccggcggcg gtaaccgcct ggcgatagac cttgtcctgg
    19501 acgtcgccgg cggcgaacac gccggggacc gaggtcgcgg tgctgtcgcc ggcggtgacg
    19561 atgtagccct cgcggtcgag cgcgagctgg cccttgaacg gcgcgctcgc cggcgtgtgg
    19621 ccgatggcga cgaacacgcc gtcgacggcg agctcggaga ccgtgcccga gttgacctcg
    19681 cgcagcttga gcccggtcac cccgggcggg tcctcgtcgc cgacgatctc ctcgaccacg
    19741 tggttccaca gcaccgtgat cttggcgttg cgcaacagcc ggtcctggtt ggtcctgtcg
    19801 gcgcgcagcg aatcgcggcg gtggatcagg gtcaccttgg tggcgaagtt ggtcaggaac
    19861 agcgcctcct cgaccgcggt gttgccgccg ccgaccaccg ccacctcgcg gccgcggaag
    19921 aagaagccgt cgcaggtcgc gcaggccgag acgccgaagc cctggaactt gcgctcgctg
    19981 tcgaggccga gccagcgcgc ctgggcaccg gtggcgatga tcagcgaatc gcccgcatag
    20041 gtatcgcgcg aatcgccctg cgccgtgaac gggtggcggg cgaggtcgac cgagaccgcg
    20101 atgtcggcga acatgcgggt gccgaccgcc ttggcctggg cggtcatctg ctccatcagc
    20161 cacggcccct ggaccacctc ggcgaagccc gggtagttct cgacgtcggt ggtgacggtc
    20221 atctggccgc cgggctgcag gccgtgcacc agcatcggct cgagcccggc gcgggcggcg
    20281 tagatggcgg cggtcaggcc ggcggggcct gagccgagga tcagcacctt ggcgtggtgg
    20341 cggtcgggca tgtcgggggc gaggctcctg gcggtcccgg gccgggcggg ctcagcggaa
    20401 cttgacgtcg acgatctcga acgacttgag gccggacggc gtctcgacgc cgacgctgtc
    20461 gccgaccgcc ttgttgatca gcgcgcgcgc cagcggcgcc gatatcgaca gccggcccag
    20521 ggcaacgtcg gactcgtcga cgccgacgat ctggtaggtc gattcctcat cggtgtcctc
    20581 gtcggccaag gtcacggtgg cgccgaactt gaccgtatcg ccggacagct tggagacgtc
    20641 gatcacctgg gcgcgcccga tcttgtcctc gagctcgcgc atgcggccct cgatgaagct
    20701 ctggcgctcg cgcgcggcat ggtactcggc gttctccgcc aggtcgccgt ggtcgcgcgc
    20761 gtccgagatc gcccggatca ccgccgggcg gtcctcggtc ttgagcttcc tcaattctgc
    20821 ttccagccgc cgaaagccgt cggcggtcat tggctctcgt tgcatgtccg gaccttgacc
    20881 gatcgcgccg ccacgcgccc cgcggggcac ccggcggcca acgctcaagt gtaggattga
    20941 agcggagcca cttcaaggct gccacggctg agcgccttga tcgcctgcac caccgcgcgg
    21001 ctgccggcaa cggtggtgta atagggcacc ttgtggatca gcgcggcgcg gcgcaggtcg
    21061 tagctgtcgg cgatcgcctg gccgctctcg gtggtattga agatcagcgc cacctggccg
    21121 tcgatgatcg catcgaccag atgcgggcgg ccctcgcgca ccttgttgat gcgccggact
    21181 tcgagcccgg cagcctgcag aaacgccgcc gtgccgctag tcgcgatcag cgtaaagccc
    21241 atctcgacca ggctccggcc cagcggcacc atcgccttct tgtcggcgtc gcgcaccgag
    21301 atgaacacgt tgcccgcatc gggcagcggc acgccggcgg cgatctgcga cttggcgaag
    21361 gcgcggccga agtcggtgtc gatccccatc acctcgccgg tcgatttcat ctccgggccc
    21421 agcagcacgt cgacgccggg gaagcgggcg aacgggaaca ccgcttcctt gaccgagaca
    21481 tgggcgagct tgcgcgcggt gaggccgaag ctcttgagct tctcgcccgc catcagccgc
    21541 gccgcgatct tggcgatcgg cacgccggtt gccttggcaa cgaatggcac cgtgcggctg
    21601 gcgcgcggat tgacctcgag gatgtacacc tgggcattgc cggcctcggt cttgatcgcg
    21661 aactggacgt tcatcaaccc gaccacgccg agcgccttgg ccagcgccac ggtctggcgc
    21721 tcgatctcgg cgatcacccc ggcgccgagc gagaacggcg gcagcgcgca cgccgaatcg
    21781 ccagagtgga tgccggcctc ctcgatatgc tccatgatgc cggcgacatg gacgtcctcg
    21841 ccgtcggcga gcgcatccac gtcgacctcg accgcgtcgg cgaggaagcg gtcgatcagg
    21901 atcggcgcgc cgccgaacag ccgcaccgcc gcgccgacat agtcgccgag cccctggcgg
    21961 tcgcggacga tctccatcgc ccgcccgcca agcacgtagg acgggcgcac cagcaccgga
    22021 tagcccagcc gctcggcgat ctcgagcgcc tgctcgaccg tgcggcagat gccgttgggc
    22081 ggctgctcga ggccgagccg ggtgagcagg ccctggaagc gctcgcggtc ctcggccagg
    22141 tcgatggcgt cgggcgaggt gccgatgatc ggcagccccg ccgcctcgag cgcgcgcgcg
    22201 agcttgagcg gcgtctggcc gccatactgc accaccagcc ccacgagccg gcccgcggac
    22261 gcctcggcgc gcgcaatccc gatcacgtcc tcggcggtca gcggctcgaa gtacagccgg
    22321 tccgaggtgt cgtagtcggt cgatacggtc tccgggttgc agttgatcat cactgtctcg
    22381 aagccggcct ggctgagcgc gtaggcggcg tgcacgcagc aatagtcgaa ctcgatgccc
    22441 tggccgatcc ggttggggcc gccgccgagg atgatcacct tgtcgcgcgc gctcggccgc
    22501 gcctcggact cgggcgcatc gggcgcctct acatcgccct cataggtcgc atacatgtag
    22561 ggcgtgcggg agtcgaactc ggcggcgcag gtatcgaccc gcttgtagac cggatggacg
    22621 ccgagccgcg cgcgcagcgc cgccacctcc gcctcgctcc ggccggtgag ctcggcgaga
    22681 cgtgcgtcgg agaagcccat ccgcttgagc tcggcgagcc cgcgcgcccc gtccggcagg
    22741 ccgttcgcgc gcactgccgc ctcggccgcg acgatgcccc cgatctggcg caggaaccac
    22801 gggtcgtagc ggcaggcgcg gttgacgtcg gccaccgaca gcccttcgcg cagcgcctgg
    22861 gcgatgacca gcacccggtc ggggctcggg cgggcaagcg cggccaggat cgcgtcgcgg
    22921 tcgccgccgg ggatgtagcc cgggatctcg acctcgttga gcccggtgag accggtctcc
    22981 atcgagcgca gcgccttctg cagcgattcg gcgaagctgc gcccgatcga catggcctcg
    23041 ccgaccgact tcatcgccgt ggtcaacagc ggctcggtgc cggggaactt ctcgaaggta
    23101 aagcgcggca ccttggtcac cacgtagtcg atggtcggct cgaacgaggc cggggtgacg
    23161 ccggtgatgt cgttctggat ctcgtcgagc gtgtagccga ccgcgagctt ggccgcgacc
    23221 ttggcgatcg ggaagccggt cgccttcgag gcgagcgcgg acgagcgcga gacgcgcggg
    23281 ttcatctcga tcaccaccat ccggcccgag tcgggatcga ccgcgaactg cacgttcgag
    23341 ccgccggtct cgacgccgat ttcgcgcagc accgcgatcg aggcggtgcg catgatctgg
    23401 tattccttgt cggtcagggt cagcgccggc gccaccgtga tgctgacgcc ggtatgcacc
    23461 cccatcgggt cgacgttctc gatcgagcag acgatgatgc agttgtccgc gcggtcgcgg
    23521 atcacctcca tctcgtactc cttccagccc agcaccgact gctcgatcag cacctcgccc
    23581 gcgggcgagg cgtcgagccc gccggcgacg atctggtcga actcctcgcg gttgaaggcg
    23641 acgccgccgc cggtgccgcc gagggtgaac gaggggcgga tgatcagcgg caggccgatc
    23701 tcgtcgagcg cccgccgcgc ctcctcgggc gcgtgcgcga ccaggccgcg cggcgtctcc
    23761 agcccgatat tggccatcgc cgcctggaac aggtcgcggt cctcggcctt ggcgatcgcc
    23821 tcgcggctgg cgccgatcag ctcgatcccg tagcgggcga gcgtgccgtt ctcggcgagc
    23881 gcgaaggcga cgttgagcgc ggtctgcccg cccatggtcg gcagcagcgc gtcgggccgc
    23941 tcgcgggcga tgatcttggc caccatctcg ggcgtgatcg gctcgatata ggtggcgtcc
    24001 gccatgcccg ggtcggtcat gatggtcgcc gggttcgagt tcaccagcac cacccggtag
    24061 ccctcggcct tgagcgcctt gcacgcctgg gtgcccgagt agtcgaactc gcacgcctgg
    24121 ccgatcacga tcgggcccgc gccgatgatc aggatcgaag agatgtcctc gcgccgtggc
    24181 atg
//
LOCUS       DASWHU010000323         6192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2061817, whole genome shotgun sequence.
ACCESSION   DASWHU010000323 DASWHU010000000
VERSION     DASWHU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061817"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGB88_12185"
     CDS             complement(<1..390)
                     /locus_tag="VGB88_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9648253.1"
                     /translation="MTIRNLQFMFKPASVALIGASQRPGTVGSVVARNLFRAGFAGPV
                     MPVNPHYQAIEGVLTYPDVASLPLVPELAVIGTPPDTVPGIIGELGARGTKAAIIITA
                     GFAESGASGRLLQQAVLDAAKPHLLRIV"
     gene            480..2261
                     /gene="acsA"
                     /locus_tag="VGB88_12190"
     CDS             480..2261
                     /gene="acsA"
                     /locus_tag="VGB88_12190"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9648254.1"
                     /translation="MKVETRGRWPIIRKRPGGGAAANLADYDRARASFSWAAARAELD
                     GLPGGRGLNIAHEAVDRHAAGSLRDQIALRWLGRDGTVRDHSYRDLAALSGRFANLLQ
                     GLGVAKGERVFALSGRVPELYVAALGTLKAGSVFCPLFSAFGPEPIHARMSIGGAKVL
                     VTSDRLYRRKVAGLRDSLPELEHVLIFGHDGALPDGPGIHDLHALMDAADAEFEIPPT
                     EPEDMALLHFTSGTTGKPKGAVHVHGAVVAHHATGRFALDLRAGDVFWCTADPGWVTG
                     TSYGIIAPLAIGATMIVDEGEFDAERWYRILEAEKVAVWYTAPTAIRMMMKLGAELPR
                     TFDHSSLRFMASVGEPLNPEAVVWGQDAFGMPFHDNWWQTETGGIMIANYAAMDIKPG
                     SMGKPLPGIEAAIVARGEGGTVRVIDAPDEDGELALKPGWPSMFRAYLHEDERYRKCF
                     AGGWYLTGDLARRDGDGYYWFVGRADDVIKTAGHMVGPFEVESALMEHAAVAEAGVIG
                     MPDPIAGEVVKAFVALKPGHEWSAALERELLAHARRLLGPAVAPKEIAFKPDLPKTRS
                     GKIMRRLLKARELGLPEGDISTLEGGA"
     gene            2258..3256
                     /gene="pdhA"
                     /locus_tag="VGB88_12195"
     CDS             2258..3256
                     /gene="pdhA"
                     /locus_tag="VGB88_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX9648255.1"
                     /translation="MSSGATPPAALERTHALELLHGMVRIRRFEEACAEHYSAGSIRG
                     FLHLYIGEEAIAVGVMRALEPADSVIATYREHGHALARGIPAAKIMAEMFGKQEGCSR
                     GRGGSMHLFDAATRFYGGNAIVGGGLPLAVGLALADKMQGRPQLAACFFGEGAVAEGE
                     FHESLNLAALWRLPVLFLCENNLYAMGTRLDLSEAQTDIALKARSYGIEAESVDGMDV
                     LAVEAAAARAAAAVRAGAGPCLLELRTYRFRAHSMFDPELYRDKAEVEEWKKRGPVVA
                     FIASLKELGLATEGDLADIEARVAAEVENAVAYAEAGSWEPVEDLLRDVYTEPAAQ"
     gene            3253..4257
                     /locus_tag="VGB88_12200"
     CDS             3253..4257
                     /locus_tag="VGB88_12200"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9648256.1"
                     /translation="MTAAANKPKVRTLSFREALREALREALAADSRVFLMGEDVGRYG
                     GCYAVSKGLLAEFGPERVRDTPLSESAFVGAGIGAALGGMRPIVEIMTVNFSLLALDQ
                     IVNNAATIRHMSGGQFSIPLVIRMATGAGRQLAAQHSHSLEGWYAHIPGIRVVTPATL
                     EDARGMLASALACPDPVLMFEHAGLYNMEGELRPDAGPVDIDRAAVRRRGADVTLITY
                     GGSLHKTLDAAERLAAGGVDAEVIDLRTLRPLDQETIVESVRRTRRAVIVDEGWRSGG
                     VSAEISARIMEAAFYDLDAPVGRVCGEEVPIPYAKHLEDAALPQPDKIVAAAQATLGG
                     "
     gene            4263..5423
                     /locus_tag="VGB88_12205"
     CDS             4263..5423
                     /locus_tag="VGB88_12205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9648257.1"
                     /translation="MAEFRMPSLGADMEAGTLVEWRVAPGDRVARGDIVAVVETQKGA
                     IEVEIFDDGEIESLLVEPGEKVPVGTPLARLSGGAAGETAVRPVPEPRAEAKPPTLPP
                     RPTPAAPAAGAGRLRSSPAARRRAAALGVELAAVAGSGPQGAITVADVEAAAGAAAKP
                     APAGERRKGMREAIAAAMSRAKREIPHYYLAATIDLAPALAWLEAENDHRPPTERLLY
                     SVLLLKAVARALRDFPELNGFYADGERRASEAIHVGVAIALRGGGLIAPALADTDGKD
                     VATLMREFRDLVARARVGGLRSSELGAATITVTSLGERGVETIFPVIYPPQVAIVGFG
                     SVVERPWSLAGEIVSRRLITASLAADHRVSDGHRGALFLERLADLLKEPEKL"
     gene            5420..5680
                     /locus_tag="VGB88_12210"
     CDS             5420..5680
                     /locus_tag="VGB88_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9648258.1"
                     /translation="MTEDEIRTAVVEQLVSVAPDIDAGDIDPAASFRDQFDIDSMDFL
                     NFVIALHERLGVDIPEIDYPKLSSLDGCVAYLAGKLAQGAAP"
BASE COUNT          886 a         2197 c         2231 g          878 t
ORIGIN      
        1 gacgatcctg agcaggtgcg gcttggcggc gtcgagcacc gcctgctgga ggaggcggcc
       61 gctggcgccg ctctcggcga agccggcggt gatgatgatc gcggccttgg tgccgcgcgc
      121 gccgagctcg ccgatgatgc cgggcaccgt gtcggggggc gtgccgatga ccgcgagctc
      181 gggcaccagc ggcaggctgg cgacgtcggg ataggtgagc acgccctcga tcgcctggta
      241 gtgcggattg accggcatga ccgggccggc gaagccggcg cggaacaggt tgcgtgccac
      301 caccgagccg accgtcccgg gccgctggct ggcgccgatc agcgccaccg aagccggctt
      361 gaacatgaac tggagattgc ggatggtcat gccggtttcc ccacgtcgca ccaacacagg
      421 taaaggaaac gcggcgccgg ttgaacgaca gaatgcgaca tgatcagatt tcccgcgcca
      481 tgaaagtcga gacacgcggt cgctggccga tcatccgcaa gcgccccggc gggggtgcgg
      541 cggcgaacct cgccgactat gaccgggcgc gcgcgtcgtt ttcgtgggcc gcggcgcgcg
      601 ccgagctcga cgggctgccc ggcggccgcg gcctgaacat cgcccacgag gccgtcgacc
      661 gccacgccgc gggctcgttg cgcgaccaaa tcgcgctgcg ctggctcggg cgcgacggca
      721 ccgtgcgcga tcacagctat cgcgacctcg ccgcgctcag cggccgcttc gccaatctgc
      781 tgcagggcct cggcgtcgcc aagggcgagc gcgtgttcgc gctctccggg cgcgtgcccg
      841 agctgtacgt cgccgcgctc ggcaccctca aggcgggcag cgtgttctgc ccgctgttct
      901 cggccttcgg gccggagccg atccacgccc gcatgtcgat cggcggcgcc aaggtgctgg
      961 tcaccagcga ccgcctctac cggcgcaagg tcgccggctt gcgcgactcc ttgcccgagc
     1021 tcgagcacgt gctgatcttc ggccacgacg gcgcgttgcc cgacggtccc ggcatccacg
     1081 acctgcatgc gctcatggat gcggccgacg ccgagttcga gatcccgccc accgagcctg
     1141 aggacatggc gctgctgcac ttcaccagcg gcaccacggg caagcccaag ggcgcggtcc
     1201 acgtccacgg cgcggtcgtc gcccaccacg ccaccggccg cttcgccctc gacctgcgcg
     1261 cgggcgacgt gttctggtgc accgccgatc cgggctgggt taccggcacc tcctacggca
     1321 tcatcgcgcc gctcgccatc ggcgcgacca tgatcgtcga cgagggcgag ttcgacgccg
     1381 agcgctggta tcgcatcctc gaggccgaga aggtcgccgt gtggtacacc gcgccgacgg
     1441 cgatccggat gatgatgaag ctgggcgctg agctgccgcg cacgttcgac cactcaagcc
     1501 tgcgcttcat ggccagcgtc ggcgagccgc tcaaccccga agcggtggtg tgggggcagg
     1561 acgccttcgg catgccgttt cacgacaact ggtggcagac cgagaccggc ggcatcatga
     1621 tcgccaacta cgccgccatg gacatcaagc cgggttccat gggcaagccg ctgcccggca
     1681 tcgaggcggc gatcgtcgcg cgcggcgagg gcggcacggt gcgcgtgatc gacgcgccgg
     1741 acgaggacgg cgagctcgcg ctcaagcccg gctggccgtc gatgttccgc gcctatctgc
     1801 acgaggacga gcgctatcgc aaatgcttcg ccggcggctg gtacctcacc ggcgacctcg
     1861 cccggcgcga tggcgacggc tactattggt tcgtcggccg cgccgacgac gtgatcaaga
     1921 ccgctggcca catggtcggg ccgttcgagg tggagagcgc gctgatggag catgcggcgg
     1981 tggccgaggc cggggtgatc ggcatgcccg acccgatcgc cggcgaggtg gtcaaggcgt
     2041 tcgtggccct caagccgggc catgaatgga gcgcggcgct cgagcgcgag cttctcgccc
     2101 acgcccgccg cctgctcggg ccggcggtgg cgcccaagga gatcgcgttc aaaccggacc
     2161 tgcccaagac gcgcagcggc aagatcatgc gcaggctgtt gaaggcgcgc gagctcggcc
     2221 tgcccgaggg cgacatctcg accctggagg gcggcgcgtg agctccggcg cgacgccgcc
     2281 cgccgccctc gagcgcacgc acgcgctcga gctgctgcac ggcatggtgc gcatccgccg
     2341 cttcgaggag gcctgcgccg agcattacag cgccggcagc atccgcggct tcctgcatct
     2401 ctacatcggc gaggaggcga tcgccgtcgg ggtgatgcgc gcgctcgagc ccgccgattc
     2461 ggtgatcgcc acctatcgcg agcacgggca cgcgctcgcg cgcggcattc ctgcggccaa
     2521 gatcatggcc gagatgttcg gcaagcagga gggctgcagc cggggccgcg gtggctcgat
     2581 gcacctgttc gacgcggcga cgcgcttcta tggcggcaac gccatcgtcg gcggcgggct
     2641 gccgctggcg gtgggccttg cgcttgccga caagatgcag ggccggccgc agctcgcggc
     2701 gtgctttttc ggcgaaggcg cggtcgccga aggcgagttc cacgaatcgc tcaacctggc
     2761 cgcgctgtgg cgcctgccgg tgctgttcct ttgtgagaac aatctctacg ccatgggcac
     2821 gcgcctcgac ctgtccgagg cccagaccga catcgcgctg aaagcgcgca gctacggcat
     2881 cgaggccgag tcggtcgacg gcatggacgt gctcgcggtc gaggcggcgg ccgcgcgcgc
     2941 ggcggcggcg gtgcgcgcgg gcgcgggccc ctgtctgctc gagctgcgca cctatcgctt
     3001 ccgcgcccat tcgatgttcg atcccgagct ctaccgcgac aaggccgagg tcgaggaatg
     3061 gaagaagcgc ggcccggtgg tcgcgttcat cgcctctctc aaggagctcg gcctcgccac
     3121 ggagggcgac ctcgcggata tcgaggcgcg tgttgcggcc gaggtcgaaa acgccgtcgc
     3181 ctacgccgag gccggcagct gggagccggt ggaggacctg ctgcgcgacg tctacacgga
     3241 gccggcggcg caatgaccgc ggcggcaaac aagccgaagg ttcgcacgct cagcttccgc
     3301 gaggcgctgc gcgaggcgtt gcgcgaggcg ctggcggcgg attcgcgcgt gttcctgatg
     3361 ggcgaggacg tcggccgcta cggcggctgc tacgcggtga gcaaggggct gctggccgag
     3421 ttcgggcccg agcgcgtccg cgacacgccg ctgtcggagt cggccttcgt cggcgccggc
     3481 atcggcgccg cgctcggcgg catgcgcccg atcgtcgaga tcatgaccgt caacttcagc
     3541 cttctcgcgc tcgaccagat cgtcaacaac gccgccacca tccggcacat gtcgggcggc
     3601 cagttctcca tcccgctggt gatccgcatg gccaccggcg ccggccgcca gctcgccgcc
     3661 cagcactcgc acagcctgga gggctggtac gcgcacatcc ccggcatccg cgtggtcacc
     3721 ccggccacgc tcgaggacgc gcgcggcatg ctcgccagcg cgctcgcctg ccccgacccg
     3781 gtgctgatgt tcgagcacgc cggcctctac aacatggagg gcgagctcag gccggacgcc
     3841 ggcccggtcg acatcgaccg cgcggccgtg cgccgccgcg gcgcggacgt cactctgatc
     3901 acctatggcg gcagcctgca caagacgctc gacgccgccg agcggctcgc cgcaggcggc
     3961 gtcgacgccg aggtgatcga cctgcgcacg ttgcgtccgc tggaccagga gacgatcgtc
     4021 gagtcggtca ggcgcacccg gcgggcggtg atcgtcgacg agggctggcg cagcggcggc
     4081 gtgtcggcgg agatcagcgc gcgcatcatg gaggcggcgt tctacgacct cgacgcgccg
     4141 gtcgggcgcg tgtgcggcga ggaagtgccg atcccctacg ccaagcatct cgaggacgcg
     4201 gcgctgcccc agcccgacaa gatcgtcgcc gcggcgcagg cgaccttggg cggctagcgg
     4261 ccgtggccga gttccgcatg ccctcgctcg gcgccgacat ggaggcgggg acgctggtcg
     4321 agtggcgcgt cgcgcccggc gaccgggtcg cgcgcggcga catcgtcgcc gtggtcgaga
     4381 cccagaaagg ggcgatcgag gtcgagatct tcgacgacgg cgagatcgag agcctgctgg
     4441 tcgagccggg cgagaaggtc ccggtcggca cgccgctcgc gcgactcagc ggcggcgcgg
     4501 cgggagagac ggcggtgcgg ccggtacccg aaccgcgcgc cgaggcgaag ccccccacgc
     4561 tgccgccgcg cccgacgccg gcggcgcccg cggccggggc ggggcgcctg cgctcctcgc
     4621 ccgcggcgcg ccggcgcgcg gcggcgttgg gggtcgagct ggcggcggtc gccggcagcg
     4681 ggccgcaggg cgcgatcacg gtcgccgacg tcgaggccgc cgcgggcgcc gcggccaagc
     4741 ccgcacccgc gggcgagcgc cgcaagggga tgcgcgaggc gatcgccgcc gccatgtcgc
     4801 gcgccaagcg cgagatcccg cactactact tggccgccac catcgacctg gcgccggcgc
     4861 tcgcctggct cgaggccgag aacgaccatc ggccgccgac ggagcggctc ctctactcgg
     4921 tacttctcct caaggcggtg gcgcgcgcgc tgcgcgactt ccccgagctc aacggcttct
     4981 atgccgacgg cgagcgccgg gcgagcgagg cgatccacgt cggcgtcgcc atcgcgctgc
     5041 gcggcggcgg cctgatcgcg ccggcgctgg ccgacaccga cggcaaggac gtggccacgc
     5101 tcatgcgcga gttccgcgac ctggtcgcgc gcgcccgcgt cggcggcctg cgcagctccg
     5161 agctgggcgc cgcgacgatc accgtcacca gcctcggcga gcgcggcgtc gagaccatct
     5221 tcccggtcat ctacccgccg caggtggcga tcgtcggctt cggctcggtg gtcgagcggc
     5281 cgtggtcgct ggcgggcgag atcgtttcgc ggcggctgat caccgccagc ctcgccgccg
     5341 accacagggt cagcgacggc caccgcggcg cgctgttcct ggagcggctg gccgacctgt
     5401 tgaaggagcc cgagaagcta tgaccgagga cgagatcagg accgcggtgg tcgagcagct
     5461 ggtgagcgtc gccccggaca tcgacgccgg cgacatcgac ccggcggcga gcttccgcga
     5521 ccagttcgac atcgattcga tggacttcct caacttcgtc atcgcgctgc acgagcgcct
     5581 cggtgtcgac atcccggaga tcgactatcc gaagctgtcg agcctcgacg gctgcgtcgc
     5641 gtacctggcg ggcaagctcg cccaaggcgc cgcgccgtga tcgagcgcta ggcggcagcc
     5701 ccttacctgc cgcctcccgc gcgcgggaag gcaatcgcat acgcgtcggt tgcttgtttg
     5761 gatggtgccc tatgtccggg cctctttcga ctcacgcgga ggcgcagagg gcgcggagtt
     5821 cgtgtgactc ggagctgccc agcacccggc actctcgcct tgtgcactcg ttcacccgcg
     5881 cgcggcggtt cggtgcgggc gcggccatgt cggcgcggag ctcgcgacta gtgcgatatg
     5941 attctatgcc cgatcatcct tgaccggtca ccccggccaa ggcgcgaatc gccgcgagct
     6001 ggggtccatc ccgccgcttg cacgagcgcc tgattagctg caaaggcgta cggaccccgg
     6061 ataacggctt cgccgtttcc ggggtgacga cttagaaaat cccagcgatt cgagcaaata
     6121 tcatcccgtt ctaatcgtcg cggcgccagc tgcccctgag gaagtagcct tccttgtcgt
     6181 agtcgaaatc ga
//
LOCUS       DASWHU010000324         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_577990, whole genome shotgun sequence.
ACCESSION   DASWHU010000324 DASWHU010000000
VERSION     DASWHU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577990"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VGB88_12215"
     CDS             complement(<1..408)
                     /locus_tag="VGB88_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX9648259.1"
                     /translation="MAAIAPPFRADHVGSLLRPAELAAVRASRAAGRIGADELGAVED
                     RCIEAAIARQAEVGLRCATDGEYRRSMWHYDFLERLDGIESFATPHGIKFHGGIETAA
                     RGLRVAGRIGFSGHPMVAHFKFVRDHARAVAKQT"
     gene            complement(421..1467)
                     /locus_tag="VGB88_12220"
     CDS             complement(421..1467)
                     /locus_tag="VGB88_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9648260.1"
                     /translation="MTAVRWSRAGLALAGALAWAGLAGAASAADVTLRLHQMLSPEAQ
                     IPAHFLVPWIDKVERESGGRIKIEHYPSMQLGGKPPQLYDQAKDGVVDIVWTVAGYTP
                     GRFPESEVFELPFMPASGEATSRALWEFYERHLTGEFADVHVLGMHVHGPGLLHVKGA
                     GIHRLEDMAGKKLRGPSRMIIKLLDSLGATPVGMPVPALPEALSRGVIDGTVIPWEIT
                     APLHIAELVNTHTEFSGTRGLYTAVFVFAMNKDSYGRLPPDLKKVIDDNSGLETSAWA
                     GRVMDEGDPPAKEIAVARGNDIVVLDQAETARWKAAAQPVIDGWIAEMNDRGLDGAAL
                     YAEAQALVAKYAGN"
     gene            complement(1569..>1986)
                     /locus_tag="VGB88_12225"
     CDS             complement(1569..>1986)
                     /locus_tag="VGB88_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9648261.1"
                     /translation="GLGWGFPAALGLCLADRDRLVVASVGDGAYMFANPVACHQIAES
                     LALPALVVILNNASWNAVRRGALEVYPSGFAARANRMPLISLAPSPDFVKIAEASRGW
                     AKRVEDGAELPAALAEAVEVVRTERRQALLDVQVAA"
BASE COUNT          280 a          733 c          703 g          270 t
ORIGIN      
        1 ggtctgcttg gcgaccgcgc gcgcatggtc gcgcacgaac ttgaagtgcg cgaccatcgg
       61 gtggccggag aagccgatcc tgccggccac gcgcaggccg cgggccgcgg tctcgatgcc
      121 gccgtggaac ttgatgccgt gcggggtggc gaagctctcg atgccgtcga ggcgctcgag
      181 gaagtcgtag tgccacatcg agcggcggta ctcgccgtcg gtggcgcatc gcagcccgac
      241 ctcggcctgg cgggcgatcg cggcctcgat gcaccggtcc tcgaccgcgc cgagctcgtc
      301 cgcgccgatc ctgcccgccg cgcgcgaggc gcgcacggcc gcaagctctg ccgggcgcaa
      361 caggctgccg acgtggtcgg cgcggaacgg cggcgcgatc gcggccacgc tcggcgccgc
      421 tcagttgccg gcgtacttgg cgaccagcgc ctgcgcctcc gcgtacaacg cggcgccgtc
      481 gaggccccgg tcattcatct cggcgatcca gccgtcgatc accggctgcg ccgcggcctt
      541 ccagcgcgcg gtctcggcct ggtcgagcac cacgatgtcg ttgccgcgcg cgaccgcgat
      601 ctccttggcc ggcgggtcgc cctcgtccat cacccgcccc gcccaggcgg aggtctccag
      661 acccgagttg tcgtcgatca cctttttcag gtcgggcggc aggcggccgt aggagtcctt
      721 gttcatggcg aacacgaaca ccgcggtgta cagcccgcgc gtgcccgaga actcggtgtg
      781 ggtattgacc agctcggcga tgtgcagcgg cgcggtgatc tcccacggga tcacggtgcc
      841 gtcgatgacc ccgcgcgaca gcgcctcggg cagcgccggc accggcatgc cgaccggggt
      901 ggcgccgagc gaatccagga gcttgatgat catgcgcgag ggcccgcgca gcttcttgcc
      961 cgccatgtcc tcgaggcggt ggatgccggc gcccttgacg tgcaacagcc ccgggccgtg
     1021 gacgtgcatg ccaagcacgt ggacatcggc gaactcgccg gtcaggtggc gctcatagaa
     1081 ctcccacagc gcgcgcgagg tggcctcgcc cgaggccggc atgaacggca gctcgaacac
     1141 ctcggactca gggaaccggc ccggggtgta gccggccacg gtccagacga tgtcgaccac
     1201 gccgtccttg gcctggtcgt agagctgcgg cggcttgccg ccgagctgca tcgaggggta
     1261 gtgctcgatc ttgatccgcc cgcccgactc gcgctcgacc ttgtcgatcc aggggacgag
     1321 gaagtgcgcc ggaatctgcg cttccggcga caacatctgg tgcaggcgaa gcgtcacgtc
     1381 cgctgccgag gccgcccccg cgaggcccgc ccaggccagc gccccggcga gcgccaggcc
     1441 cgccctcgac caccgaaccg ccgtcatcgc cgcctcctgc cgtgagccct ccccaaccgg
     1501 cgtcttggcg cgccggcgcc ccgttccgta ccacctaagc ccggcaaaat ccaaccggcg
     1561 cgcggaactc aggcggcgac ctggacgtcg aggagggcct ggcggcgctc ggtcctgacc
     1621 acctcgaccg cttcggcgag cgccgccggc agctcggcgc cgtcctcgac ccgcttggcc
     1681 cagccgcggc tggcctcggc gatcttgacg aagtcgggcg agggggcgag cgagatcagc
     1741 ggcatgcggt tcgcgcgcgc agcgaacccc gaggggtaga cctcgagggc gccgcgccgc
     1801 accgcgttcc acgatgcgtt gttgaggatc accaccaggg ccggcagcgc cagcgactcc
     1861 gcgatctggt ggcaggcgac cgggttggcg aacatgtagg cgccgtcgcc gacgctcgcc
     1921 accaccagcc ggtcgcggtc ggcgaggcag aggccgagcg cagccgggaa gccccagccg
     1981 aggccg
//
LOCUS       DASWHU010000325         6320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_194588, whole genome shotgun sequence.
ACCESSION   DASWHU010000325 DASWHU010000000
VERSION     DASWHU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194588"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGB88_12230"
     CDS             complement(<1..359)
                     /locus_tag="VGB88_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9648262.1"
                     /translation="MEGDNVVRAFSESHVERLTGLSKGQLRYWDKTGFFSPRYGYENR
                     RSPYSRVYSFRDVVGLKTLAILRKDYRVSLQHLRQAASELSRLRDDLWAGTTLYVADR
                     KVYFKEPDTGRIRGAVDR"
     gene            complement(418..1338)
                     /locus_tag="VGB88_12235"
     CDS             complement(418..1338)
                     /locus_tag="VGB88_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009605793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9648263.1"
                     /translation="MTTRRLSEQEIAHYRDQGYVVVADLLSEAVLAAIDRDIARVIDG
                     ARAVAKSNDIYDLEDTHRADRPRVRRIKQPHKHCPAVDAALRSAEIVTLVGQLVGPDI
                     RLQGTKLNMKSAEYGAPVDWHQDWAFYPHTNTDVLAVGVMLDDMTEDNGPLMVLPGSH
                     KGPVYDHHDGGFFRGAFDVAGAGLDISKAVKLKGRRGSISIHHALMVHGSSLNRSGAD
                     RRLLLFELMAADAWPLAGTATKWIDLDEFRSRMVAGELCLEPRMEAAPVRIPQPQPPG
                     VGSIYDYQATRTKRHFETYREDAPAPLPAK"
     gene            1572..2561
                     /gene="galE"
                     /locus_tag="VGB88_12240"
     CDS             1572..2561
                     /gene="galE"
                     /locus_tag="VGB88_12240"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX9648264.1"
                     /translation="MEVGGAILVTGGAGYVGSHVCKALAGAGYLPLTYDDLSTGRREL
                     VKWGPLEHGDVADRGRLAEVFLRTRPRAVIHLAALVVDAGPNFEPDNYYTTNVAGSVS
                     LLRAMAEAGIVHLVYCSAAAVYGVPESVPVAEDHPLDAITPYGTSKLMVERTIPDFAS
                     AHHMSWIALRVFNAIGADAEGDAGEWHQRETHLVPSAIEAAMGERAVLQVYGTDYGTV
                     DGTAVRDFVHVSDIAEAHLLALGHVLAGRRPAAVNIGSGRGHSVSQAIDAVEQVTGRS
                     VPVRAAPPRPGDAPVLVADRGRAAKLLGWRPRHGDLVEAVTTACRWHAGHKVR"
     gene            2561..3280
                     /locus_tag="VGB88_12245"
     CDS             2561..3280
                     /locus_tag="VGB88_12245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9648265.1"
                     /translation="MRTPPAAPAGTRIYAIGDIHGRADLLGELYRLIAADAAAVRGAG
                     RRVVVHIGDYIDRGPDSRGVIELCLAHPLAGFLPVYLKGNHEQMMLDYLDGRNDGRLW
                     VMNGGGETLTSYGIDPETTLGPPVRAALTSALPEGHRAFLDHLALSARFGDYLFVHAG
                     IDPERAIDDQDEDDMIWIRQPFLSSTTDFGCVVVHGHTITRAPAVRANRIGIDTGAFG
                     SGVLTALVLDGADRAFLHTGR"
     gene            complement(3277..3807)
                     /locus_tag="VGB88_12250"
     CDS             complement(3277..3807)
                     /locus_tag="VGB88_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX9648266.1"
                     /translation="MSRASARHLLFAAAAATLAAGPAAADMATGNIVAANGDSIGTVM
                     ATAAPQGVLLRIQIAPRGLPPGQHGMHLHQVGDCSDVGTFKMSGGHINVANREHGLLN
                     PNGPDNADLPNLIVQRDGSADVELFTTRVRLTNDDAALLDDDGSALVIHEAPDDHMTQ
                     PIGGAGARIACAPITR"
     gene            3979..4398
                     /locus_tag="VGB88_12255"
     CDS             3979..4398
                     /locus_tag="VGB88_12255"
                     /inference="COORDINATES: protein motif:HMM:NF039479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HEX9648267.1"
                     /translation="MAARPFTARSFLIRWLVAVGLVLATFNTTRFSFYRWVGDVEAGS
                     WPLKLVVGLVLVMLYIVYLRATWLSIGPIGVGLVLAFFGAVIWLMIHWGLLDPGQAKV
                     MTYVVLVVIATVMAVGISWSHIRRRVTGQADADIVGD"
     gene            4376..5722
                     /locus_tag="VGB88_12260"
     CDS             4376..5722
                     /locus_tag="VGB88_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HEX9648268.1"
                     /translation="MPTSSATDRLAGGPTASRRAVLRGLAASAVLGLARPAAAAAPAA
                     AAAVYFVPGYAPELAFFRGRPVTEQPAFTRPLPRGYDGPLTLVTRIDEAAGAVRRALM
                     PVAGHQIAVAPGAGPALFCSLNGGHFAAFDADALELVAVRRHGDGFIGGGHAAFLDGG
                     RTCVVVERRAFGPYSGRPEDHYGRLVICDGATLDPLEALSCFGVAPHEVAITHDARYA
                     VVANYGKASWPEGATGEALAEVVEPSLTVIELASGRLVDKAPSTGPGLELRHVAAHGL
                     DRVFAIQAQLVAFAAAEQTMSDWDGVYEADVWSAGPDLGYLPAPLARFDLDRDPPRAA
                     PSITADARLMRYGQSIVYDPVHDQAIATYPTRHCVVAFDGATGAVRHVLHTDGIGLRQ
                     PRGLALHPDGRHYAVSGYWHDIAVIERGSHRPVPGRAQHATLFGHSHMTAAAAKRG"
     gene            5762..>6320
                     /locus_tag="VGB88_12265"
     CDS             5762..>6320
                     /locus_tag="VGB88_12265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9648269.1"
                     /translation="MIGLRVGMPAAAAVLCAVAALAEPLPSAAPEEVGLSGARLAQIG
                     EVMGAGIEAGEIPGAVVLVVRHGRVAYYEAFGWRDRASGAPMARDAIFRIYSMTKPLV
                     SVGAMMLHEQARLYPSEPLAKYVPAFETMAVGVESFDPVTGRQGFHTEPARRAITIHD
                     LLRHTSGLTYGFFGTSRVKDMIKEAG"
BASE COUNT          842 a         2231 c         2273 g          974 t
ORIGIN      
        1 cggtccaccg caccccgaat cctgccggta tcgggttcct tgaaatacac ttttcggtcc
       61 gcgacataca gtgttgtgcc ggcccaaaga tcatcccgca gccggctcag ctcggaggca
      121 gcctgccgca ggtgctgtag cgacacgcga tagtccttgc ggaggatggc gagcgtcttg
      181 aggccaacta catcacgaaa ggagtagaca cgactgtacg ggctgcgccg gttctcgtaa
      241 ccataacgcg ggctgaaaaa tcccgttttg tcccagtagc ggagctgacc tttggacagg
      301 ccggtcagcc gttcgacgtg gctctcactg aacgcacgaa caacattgtc gccttccatg
      361 cttgtccctg cgcgcatgtc ggagcgtttc atccctgttg ccccgggaac ccgatcgtta
      421 cttcgccggc agcggcgccg gggcgtcctc gcggtaggtc tcgaagtggc gcttggtgcg
      481 ggtcgcctgg tagtcgtaga tcgagccgac gccgggcggc tgcggctgcg ggatgcgcac
      541 cggcgcggcc tccatgcgcg gctcgaggca cagctcgccc gcgaccatgc gcgagcggaa
      601 ctcgtcgagg tcgatccact tggtggcggt tcccgcgagc ggccaggcgt cggccgccat
      661 cagctcgaac agcagcagcc ggcggtcggc gccggagcgg ttgaggctcg agccgtgcac
      721 catcagcgcg tggtggatgc tgatcgagcc gcgccggccc ttgagcttga ccgccttcga
      781 gatgtcgagc cccgcgcctg ccacgtcgaa ggcgccgcgg aagaagccgc cgtcgtgatg
      841 gtcgtagacc gggcccttgt ggctgccggg aagcaccatc agcgggccgt tgtcctcggt
      901 catgtcgtcg agcatcacgc ccaccgccag cacgtcggtg ttggtgtggg gatagaacgc
      961 ccagtcctgg tgccagtcga ccggagcccc gtactcggcc gacttcatgt tgagcttggt
     1021 cccctgcagg cggatgtccg gcccgaccaa ctggccgacg agggtgacga tctcggccga
     1081 gcgcagcgcc gcgtcgaccg ccgggcagtg cttgtgcggc tgcttgatcc ggcgcacccg
     1141 cggccggtcc gcccggtgcg tgtcctcgag gtcgtagatg tcgttgctct tggccaccgc
     1201 gcgcgcgccg tcgatgacgc gcgcgatgtc gcggtcgatc gccgccagca ccgcctcgga
     1261 caacaggtcc gcgacgacga cgtagccctg atcgcgatag tgcgcgatct cctgctcgct
     1321 cagcctgcgc gtggtcatgc tcgtgtgcct cgatcacggc ccgtggtccg cggcggcacc
     1381 cgggcgggga tgatcgataa cgatagcaag ccgggccgcg cccggtcaac gccgcgcccc
     1441 gatcacggcc cggcgcggcc gggaaagttg agattggcgc tgccagagcc aatcttatct
     1501 gctaagaccg gcggcgcgcg ccgccatggt agcgccggcc gatccctcaa ccaggacgtg
     1561 acgcagcggg catggaggta ggtggggcga tcctggtcac gggtggcgcc ggttacgtcg
     1621 gcagccacgt ctgcaaggcg ttggcgggcg ccggctacct gcccctgacc tacgacgatc
     1681 tcagcaccgg ccggcgcgag ctggtcaagt ggggtccgct cgagcacggc gacgttgccg
     1741 accgcgggcg cctggccgag gtgttcctgc gcacccgccc gcgggcggtg attcacttgg
     1801 ccgcgctggt cgtcgatgcc ggcccgaact tcgagcccga taactattat acgactaacg
     1861 ttgccggcag cgtcagcctg ctcagggcga tggccgaggc cgggatcgtg cacctggtct
     1921 attgcagcgc cgccgcggtc tacggcgtgc cggaatccgt gccggtggcc gaggatcatc
     1981 cgctggatgc gatcacgccc tacggcacca gcaagctgat ggtcgagcgc acgatccccg
     2041 atttcgccag cgcccaccac atgagctgga tcgcgctcag ggtgttcaat gccatcggcg
     2101 ccgatgcgga gggcgacgcc ggcgagtggc accagcgcga gacccacctg gtccccagcg
     2161 cgatcgaggc ggcgatgggc gagcgcgcgg tgctgcaggt gtacggcacc gactacggca
     2221 ccgtcgacgg caccgcggta cgcgacttcg tgcacgtctc ggacatcgcc gaggcgcacc
     2281 tgctggcgct cggccacgtg ctcgccggca ggcgaccggc cgcggtcaac atcggctcgg
     2341 gccggggcca ctcggtcagt caggcgatcg acgccgtcga gcaggtgacc gggcgcagcg
     2401 tgccggtgcg cgcggcgccg cccaggccgg gcgacgcgcc ggtgctggtc gccgaccgcg
     2461 ggcgggcggc gaagctgctg gggtggcggc cgcgccacgg cgacctggtc gaggcggtga
     2521 ccaccgcctg ccgctggcac gcgggccaca aggtgcgctg atgcgcacgc cgcccgccgc
     2581 ccccgcgggc acgcgcatct acgccatcgg cgacatccac ggccgcgccg acctgctcgg
     2641 cgagctctac cgcctgatcg cggccgatgc cgcggcggtg cgcggcgccg ggcgccgcgt
     2701 ggtggtccat atcggcgact acatcgaccg cgggcccgac agccgcggcg tcatcgagct
     2761 ctgcctcgcg cacccgctcg ccggtttctt gccggtctat ctcaagggca atcacgagca
     2821 gatgatgctc gactacctcg acggccgcaa cgacggccgg ctgtgggtca tgaacggcgg
     2881 cggcgaaacg ctgaccagct acggcatcga tccggagacg acgttagggc cgccggtgcg
     2941 cgcggcgctc acctcggcgc tgcccgaagg ccatcgcgcc ttcctcgacc acctggcgct
     3001 gagcgcacgc ttcggcgact acctgttcgt ccacgccggc atcgaccccg agcgggcgat
     3061 cgacgaccag gacgaggacg acatgatctg gatccgtcag ccgttcctgt cctcgaccac
     3121 ggacttcggc tgcgtggtag tgcacggcca caccatcacc cgcgccccgg cggtgcgcgc
     3181 caaccgcatc ggcatcgaca ccggcgcgtt cggctcaggg gtgctgaccg cgctggtgct
     3241 cgacggcgcg gaccgggcgt tcctgcacac cggccgctag cgggtgatcg gcgcgcaggc
     3301 gatgcgcgca ccggcgccgc cgatcggctg ggtcatgtga tcgtcggggg cctcgtggat
     3361 caccagcgcc gagccgtcgt cgtcgagcag tgccgcgtcg tcgtttgtca gcctcacccg
     3421 ggtggtgaac agctcgacgt cggccgagcc gtcgcgctga acgatcaggt tgggcaggtc
     3481 ggcgttgtcg gggccgttcg ggttgagcag gccatgctcg cggttggcga cgttgatgtg
     3541 gccgcccgac atcttgaagg tgccgacgtc gctgcagtcc ccgacctggt gcaggtgcat
     3601 cccgtgctgg ccgggcggca ggccgcgggg ggcgatctgg atgcgcagca acacgccctg
     3661 gggcgcggcc gtggccatga ccgtgccgat cgaatcgccg ttggcggcga cgatgttgcc
     3721 ggtcgccata tcggcggcgg ccggtccggc ggcaagcgtc gccgccgcgg cggcgaacag
     3781 cagatgtctg gcggatgcgc gtgacatgga ctccactccc tggtttcggc gaccatcggc
     3841 cgcgacggcg caatcgtcgc cgtcggccgc gcccgggtca accgccgccc gccccgcgcc
     3901 ccggccggct tgcgtggcgc ggcgggctct gggttagact ggccggacac ggagccattg
     3961 tagcggggag atcgcgccat ggccgcaagg ccgttcaccg cccggagctt cctgatccgc
     4021 tggctggtcg cggtggggct ggtgctggcg accttcaaca ccaccaggtt ctcgttctat
     4081 cgctgggtcg gcgacgtcga ggcgggcagc tggccgctga agctggtcgt cggcctggtg
     4141 ctggtgatgc tctatatcgt ctacctgcgc gcgacctggc tgtcgatcgg gccgatcggg
     4201 gtcgggctgg tgctggcgtt cttcggcgcg gtgatctggc tgatgatcca ctgggggctg
     4261 ctcgatcccg gccaggccaa ggtcatgacc tatgtggtgc tggtcgtgat cgcgaccgtg
     4321 atggcggtcg gcatctcgtg gtcgcacatc cgccgccggg tgaccggcca ggccgatgcc
     4381 gacatcgtcg gcgactgacc gcctcgccgg cggccccacc gcctcccggc gggcggtgct
     4441 gcgcgggctc gccgcgagcg cggtccttgg cctcgcccgg ccggccgcgg cggcggcccc
     4501 cgcggcggcg gccgcggtct acttcgtgcc cggctacgcg cccgagctcg ccttcttccg
     4561 cggccggccg gtgaccgagc agcccgcgtt cacccgcccg ctgccgcgcg gctatgacgg
     4621 ccccctcacc ctggtcacgc gcatcgacga ggcggccggc gcggtgcggc gggcgttgat
     4681 gccggtcgcc ggccaccaga tcgcggtcgc ccccggggct gggccggcgc tgttctgcag
     4741 cctcaacggc ggccatttcg ccgccttcga tgcggacgcc ctggagctgg tcgccgtccg
     4801 ccgccacggc gacggcttca tcggcggcgg ccacgcggcg ttcctcgacg gcgggcggac
     4861 ctgcgtggtg gtcgagcgcc gcgccttcgg cccctattcc ggccggcctg aggaccatta
     4921 cgggcggctg gtaatctgcg atggcgccac actcgatccg ctcgaggcgc tgtcctgctt
     4981 cggcgtcgcg ccgcacgagg tcgcgatcac gcacgacgcg cgctacgccg tggtcgccaa
     5041 ctacggcaag gccagctggc ccgaaggagc cacgggcgag gcgctggccg aggtggtcga
     5101 gccgtcgctg accgtgatcg agctcgccag cggccgcctg gtcgacaagg cgccgagcac
     5161 cggccccggc ctcgagctcc gccacgtcgc ggcgcacggg ctggaccggg tgttcgccat
     5221 ccaggcgcag ctcgtcgcct tcgccgcggc cgagcagacc atgtccgact gggacggggt
     5281 ctacgaggcg gacgtctggt cggcgggccc cgacctcggc tatttgccgg cgccgctcgc
     5341 gcgcttcgac ctcgaccgcg atccgccgcg ggccgcaccg tcgatcaccg cagacgcgcg
     5401 cctgatgcgc tacggccaga gcatcgtcta cgacccggtt cacgaccagg cgatcgccac
     5461 ctacccgacg cggcattgcg tggtggcgtt cgacggcgcg accggcgccg tccgccacgt
     5521 gctccatacc gacggcatcg ggctcaggca gccgcgcggc ttggcattgc atccggacgg
     5581 gcgccattac gcggtctcgg gctactggca cgatatcgcg gtcatcgagc gcggatcgca
     5641 ccggccggtg cccgggcgcg cgcagcacgc caccctgttc ggccacagcc acatgaccgc
     5701 ggcggcggcg aaacgcgggt aagctgcacc ggttcgcggg gcgggaaaag ggaggcgaga
     5761 gatgatcgga cttcgcgtcg gcatgccggc ggcggcggcg gtgctgtgtg cggtcgccgc
     5821 gctcgccgag ccgctgccgt cggctgcgcc cgaagaggtg ggcctgtcgg gcgcgcgcct
     5881 ggcgcagatc ggcgaggtga tgggcgccgg catcgaggcc ggcgagatcc cgggcgcggt
     5941 ggtgctggtc gtgcgccacg ggcgcgtcgc ctactacgag gcgttcggct ggcgcgaccg
     6001 cgcgagtggt gcgccgatgg cgcgcgatgc gatcttccgc atctactcga tgaccaagcc
     6061 gctggtcagc gtcggcgcga tgatgttgca cgagcaggcg cggttgtacc cgtccgagcc
     6121 gctggccaag tacgtgcccg cgttcgagac catggcggtc ggggtggaga gcttcgaccc
     6181 ggtcaccggc cgccagggct tccataccga gcctgcgcgc cgcgccatca ccatccacga
     6241 cctgctccgc cacacctcgg gcctgaccta cggcttcttc ggcacctcgc gggtcaagga
     6301 catgatcaag gaggcgggcc
//
LOCUS       DASWHU010000326         5462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_938588, whole genome shotgun sequence.
ACCESSION   DASWHU010000326 DASWHU010000000
VERSION     DASWHU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938588"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VGB88_12270"
     CDS             complement(<1..643)
                     /locus_tag="VGB88_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9648270.1"
                     /translation="MHRRNFIAGLLAAGLALAPAASAWAQAATPAPLPEADRALLARI
                     EGYLDGLTTLRARFTQANADGSEDHGVLWLARPGHARIEYAPPTDILVVADGTWLIYY
                     DAELDQVSHVPLGTGPFRFLLEDDVKLSGEVAVTSLEVGAGLVRVTLVDRERADEGRV
                     TLVFDAEPLSLRQWQVLDAQGFLTTVTLDDQVAGESYPRRMFYFPDSARKRDFR"
     gene            complement(695..1705)
                     /locus_tag="VGB88_12275"
     CDS             complement(695..1705)
                     /locus_tag="VGB88_12275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9648271.1"
                     /translation="MTLLVGRPGRAAAAAGAAEVVMRDGDIAVPAAKARAGALARALG
                     GGDRQVAEVAYAAGEIAAGLVTQGGGALCVRAIEREGRIGIEVSGVSATDRPAARPEV
                     VCRSMDEVRLAAPAGARGGLELTCRRWLAPRLRAAGAHALSLDVGVATRALPGQAENG
                     DAFLMEQWRDRVLVAVIDGFGHGPPACQAAQAARRYLEGQRQLELDRMIAGTGEACRD
                     CLGVVLSVLRFDCVRGLVEHAAVGNVVLRRWREGVSSRDPLRRGTLGVGTPVSLITVR
                     AFRPDDIFVLYSDGVAARWDWRWANEVRGVPAAAAARLLLDRYDRADDDATVAVVKVA
                     GH"
     gene            complement(1890..4235)
                     /locus_tag="VGB88_12280"
     CDS             complement(1890..4235)
                     /locus_tag="VGB88_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9648272.1"
                     /translation="MSARSQAMRGAPLLPRGAGAALRRQLVAATAVAAGAAALLMLAA
                     LASYAPGDPSWNTAAGGPAANLLGRAGAHFADAALQAFGIAAAVPALALIAWGWRLWR
                     DGRLAGPWLRLSAAVLAMLALPAAAAAAVPAVSWPSHAGAGGVVGRFLTAELAALVAP
                     AGLGRWPVVAGCGLAAVALVLAALGLRREEWRRMAAAGALALGQLWQAAAALWAASGE
                     LAARRLARGPQVMPAPGELPREVVRPAPPRAAGSRRAAARVVGAEAARTGARELAERQ
                     GRLELDAGAGFRLPPLGLLAEPDARARALAADDEALAQNARMLETVLADFGVRGEIVR
                     VRQGPVVTRYELEPAPGTRAARVVALADDIARSMSAISVRVAVIPGQNAIGIELPNAR
                     REIVYLRLLLGSHDYERAPAELPLALGKDISGAPVIVDLARMPHLLVAGTTGSGKSVA
                     VNSMILSLLYRLAPEQCRLILIDPKMLELSIYDDIPHLLHPVVVDPAKAVTALKWAVK
                     EMNERYRMMSFLNVRNIAGYNRRLAEAARKGERLERTVQTGFDAETGRPLYESQPIDM
                     TPLPFIVVVVDEMADLMLVAGKDIEITIQSLAQKARAAGIHLIIATQRPSVDVITGTI
                     KANLPTRISFQVTSKIDSRTILGEQGAEQLLGMGDMLYMAGGGQVIRVHGPFVSDQEV
                     EEVANFLRAEGEPDYLDAVTEDADDDPLAGPGEGGDDLFDQAVALVARERKASTSFIQ
                     RQLSIGYNRAAKIIERMEAEGMVGQANHVGRREVLLPDHGA"
     gene            complement(4261..>5462)
                     /locus_tag="VGB88_12285"
     CDS             complement(4261..>5462)
                     /locus_tag="VGB88_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9648273.1"
                     /translation="NDRLANLVDYPFRRLAALLADTAPPAGLSPINMAIGEPQHPYPA
                     LVAEVLAANAHLWGRYPPTVGTPAFRAAAAGWLSRRFALPEGMIEAERHVLPCNGTRE
                     ALFTVALLAVPQAVAGAKPVVLMPDPFYQVYAGAAVMAGAEPVFVAATAETGFLPDFA
                     ALPADTLARAALAYLCTPANPQGTVAGRDYLAGLVALARAADFVLAIDACYCDVYTGA
                     APVGVFEACATDAKGLDHVIAFHSLSKRSSVPGLRSGFVAGDPALMAEYARLRSYTGG
                     ASPLPVIATATALWGDEAHVEANRALYQRKFALAAERLRGRCGFYVPPGGFYLWLEVG
                     DGEAAAQRLWREHGVRAMPGAYLSRGGCGGETGQAPGAPYIRLALVHDLATTEAALDR
                     LARALPG"
BASE COUNT          739 a         2053 c         2025 g          645 t
ORIGIN      
        1 cgcggaagtc gcgcttgcgg gcgctgtcgg ggaaatagaa catgcgccgc gggtagctct
       61 cgccggcgac ctggtcgtcg agggtgacgg tggtcaggaa cccctgcgca tcgagcacct
      121 gccactggcg gaggctgagc ggctcggcgt cgaacaccag ggtcacgcgg ccctcgtcgg
      181 cgcgctcgcg gtcgaccagg gtgacgcgca ccagcccggc gccgacctcg agcgaggtca
      241 ccgcgacctc gccgctgagc ttgacgtcgt cctcgagcag gaaccggaac gggccagtgc
      301 cgagcggcac gtggctgacc tggtcgagct cggcgtcgta gtagatcagc caggtgccgt
      361 cggcgaccac caggatgtcg gtcggcggcg catactcgat gcgcgcatgc cccgggcgcg
      421 cgagccacag cacgccgtgg tcctcgctgc cgtcggcgtt ggcctgggtg aagcgggcgc
      481 ggagcgtggt caggccgtcg agatagccct cgatgcgcgc cagcagcgcg cggtccgcct
      541 cgggcagcgg cgcaggcgtc gccgcctgcg cccaggccga ggccgccgga gcgagcgcga
      601 ggccggcggc gagcagcccg gcgatgaagt tgcgcctgtg catcgccgca atgtgtgggc
      661 gtgcgcgccg cggcgcaagg cgcggacacg gcggtcagtg gccggcgacc ttgaccacgg
      721 cgacggtggc gtcgtcgtcg gcgcggtcgt agcggtcgag cagcaggcgc gccgccgccg
      781 ccgccggcac cccgcgcacc tcgttcgccc agcgccagtc ccagcgcgcg gcgacgccgt
      841 ccgagtagag cacgaagatg tcgtcgggcc ggaacgcgcg caccgtgatc agggagaccg
      901 gcgtgccgac gccgagcgtg ccgcggcgca gcgggtcgcg cgaggacacc ccctcgcgcc
      961 agcggcgcag cacgacgttg ccgaccgcgg catgctcgac cagcccgcgc acgcagtcga
     1021 agcgcagcac cgacagcacc acgccgaggc agtcgcggca ggcctcgccg gtgccggcga
     1081 tcatgcggtc gagctcgagc tggcgctggc cctcgaggta gcggcgcgcc gcctgggcgg
     1141 cctggcaggc gggcgggccg tggccgaagc cgtcgatcac cgccaccagc acccggtcgc
     1201 gccattgctc catcaggaag gcgtcgccgt tctcggcctg gcccggcagc gcccgggtgg
     1261 cgacgccgac gtcgaggctg agcgcgtggg caccggcggc gcgcaggcgc ggcgccagcc
     1321 agcgccggca ggtgagctcc aagccgccgc gcgccccggc cggcgcggcc aggcgcacct
     1381 catccatcga ccggcagacc acctcgggcc tggccgccgg ccggtcggtg gcggagacgc
     1441 cgctgacctc gatgccgatc cgcccctcgc gctcgatcgc gcgcacgcag agcgcgccgc
     1501 cgccctgcgt gaccaggccg gcggcaatct cgcccgccgc gtaggccacc tccgcgacct
     1561 ggcggtcgcc gccgccgagc gcgcgcgcca gggccccggc ccgggccttg gccgcgggca
     1621 cggcgatgtc gccgtcgcgc atcaccacct cggcggcccc ggcggccgcc gccgcgcgac
     1681 cgggccgacc cacaagcaga gtcatccctc cctccttccg cgcccttggg cgagggtcgg
     1741 gcgtgggacc cgagctcccg cgcccggagc gcgcgtctac ccgtataacg tgtagatcaa
     1801 agtgatctgg tgtcgcccgg ccgggccgtc aatctcgacc gcgaaaatgt ccggtaagat
     1861 attggttccg aaatattatc gtcgtcagat caagcgccgt ggtcgggcag aaggacctcg
     1921 cggcggccga cgtggttggc ctgcccgacc atgccttcgg cctccatgcg ctcgatgatc
     1981 ttcgccgccc ggttgtagcc gatcgagagc tggcgctgga tgaagctggt cgaggccttg
     2041 cgctcacgcg caaccagcgc caccgcctgg tcgaacaggt cgtcgccgcc ctcgccgggc
     2101 ccggccagcg ggtcgtcgtc ggcgtcctcg gtgaccgcgt cgagatagtc gggctcgccc
     2161 tcggcgcgca ggaagttggc cacctcctcg acctcctggt cgctgacgaa cgggccgtgg
     2221 acgcggatca cctggccgcc gccggccatg tacagcatgt cgcccatgcc caacagctgc
     2281 tcggcgccct gctcgccgag gatggtgcgg ctgtcgatct tggaggtgac ctggaagctg
     2341 atccgggtcg ggagattggc cttgatggtg ccggtaatca cgtcgaccga cggccgctgg
     2401 gtggcgatga tcaggtggat gccggcggcg cgcgccttct gcgccaggct ctggatggtg
     2461 atctcgatgt ccttgcccgc gaccagcatc aggtccgcca tctcgtcgac caccaccacg
     2521 atgaacggca gcggggtcat gtcgatcggc tggctctcgt agagcgggcg gccggtctcg
     2581 gcgtcgaacc cggtctgcac cgtgcgctcc agccgctcgc ccttcctcgc cgcctcggcc
     2641 aggcgccggt tgtagccggc gatgttgcgc acgttgagga acgacatcat gcggtagcgc
     2701 tcgttcatct ccttgaccgc ccatttgagc gcggtcaccg ccttggccgg gtcgaccacc
     2761 accgggtgca gcaaatgcgg gatgtcgtcg tagatcgaca gctcgagcat cttggggtcg
     2821 atcaggatca gccggcactg ctcgggcgcg agccggtaca ggagcgacag gatcatgctg
     2881 ttgaccgcca ccgacttgcc cgagccggtg gtgcccgcga ccagcagatg cggcatgcgc
     2941 gccaggtcga cgatcaccgg cgcgccgctg atgtccttgc cgagcgcgag cggcagctcg
     3001 gcgggcgcgc gctcgtagtc gtgcgagccc agcagcaggc gcaggtagac gatctcgcgc
     3061 cgggcgttgg gcagctcgat gccgatcgcg ttctggcccg ggatcaccgc cacccgcacc
     3121 gagatcgcgc tcatcgagcg ggcgatgtcg tcggccagcg ccaccacccg cgcggcgcgc
     3181 gtgcccggcg ccggctcgag ctcgtagcgg gtcaccaccg gcccctggcg gacgcgcacg
     3241 atctcgccgc gcacgccgaa gtcggcgagc acggtctcga gcatgcgcgc gttctgggcc
     3301 agcgcctcgt cgtcggcggc cagcgcgcgg gcgcgcgcgt ccggctcggc gagcaggccg
     3361 agcggcggca ggcggaagcc cgcgccggcg tcgagctcga gccggccctg gcgctcggcc
     3421 agctcgcggg cgccggtgcg cgcggcctcg gcgccgacca cgcgcgccgc ggcccggcgg
     3481 ctgccggcgg cgcgcggcgg cgccgggcgc acgacctcgc gcggaagctc gcccggcgcc
     3541 ggcatgacct gcgggccgcg cgcgagccgc cgcgcggcaa gctcgcccga cgccgcccac
     3601 agcgccgccg ctgcctgcca aagttgaccg agcgcgagcg ccccggccgc ggccatccgc
     3661 cgccattcct cgcgcctgag cccgagcgcg gcgagcacca gggcaaccgc ggccagcccg
     3721 cacccggcca ccaccggcca gcggccgagg cccgccgggg cgaccagcgc cgcaagctca
     3781 gccgtgagga accggccgac cacgccgccg gcgccggcgt ggctcggcca ggagacggcg
     3841 ggcacggccg cggccgcggc cgcgggcagc gccagcatgg cgagcaccgc cgccgacagc
     3901 ctgagccacg gcccggccag gcggccgtcg cgccacagcc tccagcccca ggcgatcagc
     3961 gccagcgccg gcaccgcggc ggcgatgccg aacgcctgca gcgcggcatc ggcgaaatgc
     4021 gccccagcgc ggcccagcag attggcggcc gggccgccgg cggcggtgtt ccaggacgga
     4081 tccccgggcg cgtagctcgc cagcgcggcc agcatcagca gcgccgccgc gcccgccgcc
     4141 accgccgtcg cggccaccag ctggcggcgc agcgccgcgc ccgcgcccct gggcagcagc
     4201 ggcgcgccgc gcatcgcctg agatctcgcg ctcatgatcg tgcgctcacc gcccctcctg
     4261 tcagcccggc agcgcccgcg ccagacggtc gagcgcggcc tcggtggtcg cgaggtcgtg
     4321 gaccaacgcc aaacgaatat agggcgcgcc gggcgcctgg cccgtttcgc cgccgcaacc
     4381 accgcgactg agataggcgc cgggcatcgc gcgcacgccg tgctcgcgcc acaggcgctg
     4441 cgccgccgcc tcgccgtcgc cgacctcgag ccacaggtag aagcccccgg gcgggacgta
     4501 gaagccgcag cgcccgcgaa gccgctcggc ggcgagcgcg aacttgcgct ggtaaagcgc
     4561 gcggttcgcc tcgacatgcg cctcgtcgcc ccacagcgcg gtcgcggtcg cgatcaccgg
     4621 caaggggctc gcgccgccgg tgtagctgcg caggcgcgca tactccgcca tcagcgccgg
     4681 atccccggca acgaagcccg agcgcagccc gggcacgctc gagcgcttgg acagcgagtg
     4741 gaaggcgatc acgtggtcga ggcccttagc atcggtcgcg caggcctcga acacgccgac
     4801 cggcgccgcg ccggtgtaga cgtcgcagta gcaggcgtcg atcgcgagca cgaagtcggc
     4861 tgcgcgcgcg agtgcgacca ggccggcgag gtagtcgcgg cccgccaccg tgccctgcgg
     4921 gttggcgggc gtgcacaggt aggcgagcgc ggcgcgcgcg agcgtgtctg cgggcagcgc
     4981 cgcgaagtcg ggcaggaagc cggtctcggc ggtggcggcg acgaacacgg gctcggcgcc
     5041 cgccatcacc gcggcgccgg catagacctg atagaacggg tccggcatca gcaccaccgg
     5101 cttggcgccg gcgactgctt gcggcaccgc cagcagcgcc accgtgaaca gcgcctcgcg
     5161 ggtgccgttg cacggcagca cgtggcgctc agcctcgatc atgccttcgg gcagcgcgaa
     5221 ccggcggctg agccagcccg ccgccgcggc gcggaaggcg ggcgtgccga cggtcggtgg
     5281 gtagcgcccc cacagatggg cgttggcggc aagcacctcc gcgaccagcg ccggataggg
     5341 gtgctgcggc tcgccgatcg ccatgttgat cgggctcagg cccgcgggcg gggcggtgtc
     5401 ggcgagcagc gcggcaaggc ggcggaacgg gtagtcgacc aggttggcga gacggtcgtt
     5461 ga
//
LOCUS       DASWHU010000327         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_197086, whole genome shotgun sequence.
ACCESSION   DASWHU010000327 DASWHU010000000
VERSION     DASWHU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197086"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..949)
                     /locus_tag="VGB88_12290"
     CDS             complement(95..949)
                     /locus_tag="VGB88_12290"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX9648274.1"
                     /translation="MTEDSRPRIAFQGLAGAYSNMACLAAYPGHEPLACETFEDAFAA
                     VAGGGAALAMIPVENSLAGRVADIHHLLPDSGLNIVAEHFQRVEHHLLALPGAALEGL
                     RTVHSHVHALAQCRDRIRALGLKPVIEADTAGAAAAIAKRGEASQAAIASALAAEIYG
                     LKVLEANIEDAAHNTTRFLVMARAPEEPEPGAGPVITSFVFKVRHIPAALYKALGGFA
                     TNGVNMLKLESYMLGGAFTSAQFYCDIAGHPAEEPVRLALEELGFFTREVKILGVYPA
                     HPFRKQGG"
     gene            complement(963..1712)
                     /locus_tag="VGB88_12295"
     CDS             complement(963..1712)
                     /locus_tag="VGB88_12295"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX9648275.1"
                     /translation="MVEADEAIVLIPARLASTRLPGKVLADINGAPMIVHVWRRAVEA
                     DVGPVVVACADAAICEAVERAGGRAQPTPPELPSGSDRVCAALAALDPARRYARVVNL
                     QGDLPTIAPADIRTALALLDSPAVDIATLAAEIADDAERADPNVVKVVAGFAAGARRA
                     RALYFTRVAAPWGEGPHYHHIGLYAYRRDALERFVALAPSALERRERLEQLRALEAGL
                     RIEVALVDTVPLGVDTAADLAAARRLLAQPE"
     gene            1853..2449
                     /locus_tag="VGB88_12300"
     CDS             1853..2449
                     /locus_tag="VGB88_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9648276.1"
                     /translation="MSTIEINKFVGGFLMAMLLALGFNFIAQLLYGGTEHGREAEPVF
                     VVEPAAPAAAQPAEPAAEEVAAEPAAEAAAPAEAATEPAPDQVAAAPAASAAGDIEAG
                     AKVFKKCAACHGDSAGAGHKIGPNLWGVVGAPVAHHQDFKYSSALSGLGGTWDQARLD
                     AFLADPKSYAPGTKMAFPGLSDAGDRAAVIAYLAGRGG"
BASE COUNT          344 a          890 c          982 g          336 t
ORIGIN      
        1 gggttattgc gcggcaaccg ttgttaacgg cgctaagggg cggtaatggc gccggattca
       61 gaacttgatt ttcggaggga acgcctctgg cctctcaccc gccctgcttc cggaacgggt
      121 gcgcggggta gacgccgagg atcttgacct cgcgggtgaa gaagccgagc tcctcgagcg
      181 cgagcctcac cggctcctcg gccgggtggc cggcaatgtc gcagtagaac tgcgccgagg
      241 tgaacgcgcc gccgagcatg tagctctcga gcttgagcat gttcaccccg ttggtggcaa
      301 acccgccgag cgccttgtag agcgcggccg ggatgtggcg caccttgaac acgaagctgg
      361 tgatcaccgg gccggcgccc ggctcgggct cctcgggcgc gcgcgccatg accaggaagc
      421 gggtggtgtt gtgggcggcg tcctcgatgt tcgcctccag caccttaagc ccgtagatct
      481 cggcggcgag cgcggaggcg atcgcggcct ggctcgcctc gccgcgcttg gcgatcgccg
      541 ccgccgcgcc cgcggtgtcg gcctcgatca ccggcttgag cccgagcgcg cggatgcggt
      601 cgcggcactg ggcgagggcg tggacgtggc tgtgcacggt gcggagacct tcgagcgcgg
      661 cgccggggag cgcgagcagg tggtgctcga cgcgctggaa gtgctcggcg acgatgttga
      721 gccccgagtc cggcagcagg tggtggatgt cggcgacccg gccggcgagc gagttctcga
      781 ccgggatcat ggcgagcgcg gcgccgccgc ccgcgaccgc ggcgaaggcg tcctcgaagg
      841 tctcgcacgc cagcggctcg tggcccggat aggcggcgag gcaggccatg ttcgagtacg
      901 ccccggccag gccctggaag gcgatgcgcg ggcgcgagtc ttcggtcatc ggtcggcggt
      961 catcattcgg gctgggcgag caggcggcgg gcggcggcga ggtcggcggc agtgtcgaca
     1021 ccgaggggga ccgtgtcaac cagcgccacc tcgatgcgca ggccggcctc gagcgcgcgc
     1081 agctgctcga gccgctcgcg ccgctcgagg gccgagggcg cgagcgcgac gaagcgctcg
     1141 agtgcgtccc gccgataggc gtagagcccg atgtggtggt agtgcggccc ttccccccac
     1201 ggcgcggcca cgcgggtgaa gtagagcgcg cgcgctctcc tcgcgccggc ggcgaacccc
     1261 gccaccacct tgaccacgtt ggggtcggcg cgctcggcgt cgtccgcgat ctcggccgcg
     1321 agcgtggcga tgtcgaccgc gggcgagtcc agcagcgcga gggccgtgcg gatgtcggcg
     1381 ggcgcgatgg tcggcaggtc gccctgcaaa ttgaccacgc gcgcgtagcg gcgcgcgggg
     1441 tcgagcgcgg cgagcgcggc gcagacgcgg tccgagcccg agggcagctc cggcggggtc
     1501 ggctgggcgc gcccgccggc gcgctcgacg gcctcgcaga tcgcggcgtc cgcgcacgcc
     1561 accaccaccg ggcccacgtc cgcctcgacc gcgcgccgcc agacatggac gatcatcggc
     1621 gcgccgttga tgtcggcgag caccttgccc ggcaggcggg tcgaggccag acgggccggg
     1681 atcagcacga tcgcctcgtc cgcctcgacc atggcgcgca cagctccgcg cgaagctacc
     1741 cgggaatgcc cgccgcgcgg gcgcatagga gttgcggaag cgccggcgat gccgctaggt
     1801 tgcccgcctc ccggcgccgc cgggacgccc accatcgcag gccacggtgc gcatgtccac
     1861 gatcgagatc aacaagttcg tcggcggctt cctgatggcg atgctgctgg cgctgggctt
     1921 caatttcatc gcccagctgc tctatggcgg caccgagcac ggccgcgagg cggagccggt
     1981 gttcgtggtc gagccggctg cgccggcggc ggcgcaaccg gccgagccgg cggccgagga
     2041 ggtcgcagcc gagcccgccg ccgaggcggc cgcaccggcc gaggccgcga ccgagcccgc
     2101 tcccgatcag gtcgcggccg cgccggcggc gagcgcggcc ggcgacatcg aggcgggggc
     2161 gaaggtgttc aagaagtgcg ccgcctgcca cggcgacagc gccggcgccg ggcacaagat
     2221 cggccccaat ctgtggggcg tagtcggggc gcccgtcgcc caccatcagg acttcaagta
     2281 ttcgagcgcg ctgtccggcc tcggcggcac ctgggaccag gccaggctcg atgcgttcct
     2341 tgccgatccg aagtcgtatg cacccggcac caagatggcc ttccccgggc tttcggatgc
     2401 gggcgaccgc gccgccgtga tcgcctatct cgccggccgg ggcggctgag ggagcgcgcg
     2461 gacgccggtc acgatcgtgc gcgcgccctt gaactggggc gcgcatcggg caccttatag
     2521 ggaccggcac cggcgggcgg caccgcccca gc
//
LOCUS       DASWHU010000328         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1695149, whole genome shotgun sequence.
ACCESSION   DASWHU010000328 DASWHU010000000
VERSION     DASWHU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695149"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGB88_12305"
     CDS             complement(<1..311)
                     /locus_tag="VGB88_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648277.1"
                     /translation="MSRRGRTVGIVQARMRSERLPGKAMVELAGRPAIHYVLSRAGAA
                     KSLDELWLACTDDPGDDALAAYAEGLGVRVFRGDDADVISRFVAIARQSDAGAIVRLT
                     G"
     gene            complement(308..1351)
                     /locus_tag="VGB88_12310"
     CDS             complement(308..1351)
                     /locus_tag="VGB88_12310"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9648278.1"
                     /translation="MTVRLMITAAGGALSCQTIAYAKASARYTIEVLAVDMADTALGR
                     YAADHFATVPHGASDGYVDRICALVADYGIDLVLPCSDEEALALAAERARLEALGSTL
                     ACADIETLRVMGDKSRAYRTLAAHGLPAPAFRLAETPAALAAATEAFQAERGAFVVKP
                     PAGSRGGRDTIIVHGGVAGARPYSGGRELEMDYDTFRREFLAAAAGRLPALVCERLVP
                     PAYDVDVLARDGRALRAVPRRRLNPAGVPYRGYVVEDRPDLVALAAATAEALALSWLY
                     DFDVMQTLDGRPAILELNPRPSGSFATSIAAGVPLIDDLVALAKGEPLSDAGPLPSAL
                     TVVPQTMLKVIAA"
     gene            complement(1348..>2248)
                     /locus_tag="VGB88_12315"
     CDS             complement(1348..>2248)
                     /locus_tag="VGB88_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX9648279.1"
                     /translation="AAGADAVKFQTVFADESYQPGTESHRVFSATALSPEAMRRLNAR
                     ARARGLVPFSTPGGFRALAAMLESGMAMIKISSGLLTNWPLIERAAASGLPLVLSTGM
                     ADADEVEAALLRARAAGAQALAVLHCTSLYPAPAETLNLRAIVTLARTTGLPVGYSDH
                     FRGPLAAVAAVALGACVIEKHLTLDSSLAGADHAISAEPEAFADMVRQIRAVEAMLGD
                     GIKRAAPAEVALRSGRRRFVVARRAIPAGARLVADDLALLRCAPGAGRLPANAYHQVL
                     GARARVPIASGEPLDAAMIEGGP"
BASE COUNT          309 a          822 c          862 g          255 t
ORIGIN      
        1 tcgccggtca ggcgcacgat cgcgcccgcg tcgctctggc gcgcgatggc gacgaagcgg
       61 ctgatcacgt cggcgtcgtc gccgcggaag acgcgcacgc cgaggccttc ggcataggcc
      121 gcgagcgcat cgtcgcccgg atcgtcggtg caggcgagcc acagctcgtc gaggctcttc
      181 gcggcgccgg cgcggctcag cacgtaatgg atcgccggcc ggccggcgag ctcgaccatg
      241 gccttgccgg gcaggcgttc ggagcgcatg cgcgcctgga cgatgccgac cgtgcgcccg
      301 cgccggctca cgcggcgatc accttaagca tggtctgcgg caccacggtc aacgccgaag
      361 gcagcggccc cgcgtccgac agcggctcgc ccttggccag cgcgaccaaa tcgtcgatca
      421 gcggcacgcc ggccgcgatc gaggtggcga agctgccgct cgggcgcggg ttgagctcga
      481 ggatcgcggg ccggccgtcc aaagtctgca tgacgtcgaa gtcgtagagc cacgacagcg
      541 ccaacgcctc ggcggtggcg gcggcgagcg cgaccaggtc ggggcggtcc tcgactacgt
      601 agccgcggta ggggaccccg gcggggttga gcctgcgccg cggcaccgcg cgcagcgcgc
      661 gcccgtcgcg tgccagcacg tccacgtcgt aggccggcgg caccagccgc tcgcacacca
      721 gcgccggcag gcggccggcg gcggcggcga ggaactcgcg gcggaaggtg tcgtagtcca
      781 tctcgagctc gcgcccgccg gagtacggcc gcgcgccggc gaccccgccg tgaacgatga
      841 tggtgtcgcg cccgccgcgt gagcccgccg gcggcttgac cacgaacgcg ccgcgctccg
      901 cctggaacgc ctcggtcgcc gcggcgagcg ccgccggcgt ctcggcgagg cggaacgcgg
      961 gcgccggcag cccgtgcgcc gcaagcgtcc ggtaggcgcg ggacttgtcg cccatcacgc
     1021 gcagggtctc gatgtcggcg caggcgagcg tagagccgag cgcctcgagg cgcgcgcgct
     1081 cggcggcaag cgccagcgcc tcctcgtcgg agcacggcag caccaggtcg atgccgtaat
     1141 ccgcgaccaa cgcgcaaatt cggtcgacgt acccgtcgct cgcgccgtgg ggcacggtgg
     1201 cgaagtggtc ggcggcgtag cggccgagcg cggtgtcggc catatcgacc gccagcacct
     1261 cgattgtgta gcgcgcgctc gccttggcat aggcgatggt ctggcacgac agcgcgccgc
     1321 ccgccgcggt gatcatcagc cgcacggtca cgggccgccc tcgatcatgg cggcgtcgag
     1381 cggctcgccc gaggcgatgg ggacgcgcgc gcgcgcgccc agcacctggt ggtaggcgtt
     1441 cgccggcagg cggcccgcgc ccggcgcgca gcgcagcagc gcgaggtcgt cggcgaccag
     1501 gcgcgcgccg gccgggatcg cgcgccgggc gaccacgaac cggcgccggc ctgagcgcag
     1561 cgcgacctcg gccggggcgg cgcgcttaat gccgtcgccg agcatcgcct cgaccgcgcg
     1621 gatctggcgc accatgtcgg cgaacgcctc gggctcggcg gagatggcgt ggtcggcgcc
     1681 ggcgagcgag gagtcgagcg tgaggtgctt ctcgatcacg cacgcgccga gcgccaccgc
     1741 ggcgaccgcg gcgagcggac cccggaaatg gtctgagtag ccgaccggca ggccggtggt
     1801 gcgggcgagt gtcacgatcg cgcgcaggtt cagcgtctcg gccggcgccg ggtaaagcga
     1861 ggtgcagtgc aggaccgcga gcgcctgcgc gccggccgcg cgcgcgcgca gaagcgcggc
     1921 ctcgacctcg tcggcatcgg ccatgccggt cgacagcacc aacggcaggc cgctcgccgc
     1981 cgcgcgctcg atcagcggcc agttggtcag cagccccgag gagatcttga tcatcgccat
     2041 gcccgactcg agcatcgcgg cgagcgcgcg gaagccgccg ggagtcgaga acggcaccag
     2101 gccgcgggcg cgggcgcgcg cgttcagccg gcgcatcgcc tcgggcgaca gcgcggtcgc
     2161 ggagaacacg cggtgcgact cagtgcccgg ctgatagctc tcgtcggcga acacggtctg
     2221 gaacttgacc gcatccgccc ccgccgcg
//
LOCUS       DASWHU010000329        10881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_944040, whole genome shotgun sequence.
ACCESSION   DASWHU010000329 DASWHU010000000
VERSION     DASWHU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944040"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VGB88_12320"
     CDS             complement(<1..540)
                     /locus_tag="VGB88_12320"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9648280.1"
                     /translation="MADAELSRTDGLILSSGRFFFRRRNQVFPIVFLALLLGLRPALF
                     DDPALELANDGAALLLALAGSGFRLWTIGLAYIKRGGVDKKIHADRLVTAGMFGVSRN
                     PLYLGNALVMLGLLVAIGNAVALAVGALFFGAVYASIIVAEERYLHDRFGAEYAAYCA
                     RVPRLWPDFSLYRAAARDMT"
     gene            complement(544..1293)
                     /gene="cmoA"
                     /locus_tag="VGB88_12325"
     CDS             complement(544..1293)
                     /gene="cmoA"
                     /locus_tag="VGB88_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007534868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEX9648281.1"
                     /translation="MASEPEPTPGNVDRLFKEARAPSDFKFGRETARVFDDMVGRSVP
                     FYGEIQRMMAEIAADFATPGSSVWDLGCASGTTMAGLDAALDAGVRLIGVDNSRDMLD
                     KAREKLAASTSGRAIEFIEADIHALPVISDASVVVMCLTLQFVRPLHRSRVLDAVWHG
                     MRDNGCLILVEKLTLEDSDLNRLYISYYYDLKRRNGYAESEIAQKREALENVLIPYRF
                     DENLDLLRDTGFRSIETFFRWYNFCGLLAVK"
     gene            complement(1357..2352)
                     /locus_tag="VGB88_12330"
     CDS             complement(1357..2352)
                     /locus_tag="VGB88_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9648282.1"
                     /translation="MIEAAIVGLGWWGKKLAEVARASRHIRFTLGVEPDPATARQAAE
                     TFGFPVVPDYARALGDARIRAVVLATPHALHDDQIAAAAAAGKHVFCEKPLALTKAAA
                     AQSVARCREAGLVLGLGHERRFEPPVAEMLRAVRAGELGTVLQVEANFSHDRFTRLDA
                     GNWRLSKDNAPAGGMTATGIHVLDLATAMLGEGAFALASSETLASDIANGDSTCALVK
                     YKSGATAYVATMLATPFISRFAVYGRDGWIEIRDKAHVEAPAGWTVLRAGADGTVTET
                     DVANSEPVRDNLEAFARAAAGEAAYPIATGEMIANAAVMEAIFRSAESGRIEPVP"
     gene            2479..3612
                     /locus_tag="VGB88_12335"
     CDS             2479..3612
                     /locus_tag="VGB88_12335"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HEX9648283.1"
                     /translation="MTRNLLGRCAIAAALAATAIGSAYADTEITISSWVGPKHPVNFG
                     GYEPYIAAVEAASGGAIRFRLFTGGALLGAKATLAGIRDGIADTGVIALTYHPAEFPL
                     AQLIADLALWSDDALVAGAAVTEFNLLHCPACLDEFRAQGLVYNGTYSTAPYTIIGRR
                     PINVPADLAGLKVRTPGSVWDRWVQHMGAVPVNLPASDMYESLERGITDLVLQPVAAL
                     RSYSLWDVATDVTNLNLGTFHSLSLLGYGREAWQGYDAGVRKLLMDNAPVALIGASQG
                     YVDLDQEVIADADELKVVIHPPGEGLVAATRAFAEADRAELAEISRSRHGIAEPEPMI
                     ETFLGLLDKWRAIMAPIRDDPAKMAAALKAEVFDHLDPARHGM"
     gene            complement(3620..4924)
                     /locus_tag="VGB88_12340"
     CDS             complement(3620..4924)
                     /locus_tag="VGB88_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9648284.1"
                     /translation="MSPLTIGFAGLAVMIVLLLARLPVGVTLGVVAIAGVWLLVGERA
                     ALGLLNEIPYEFVAHWTLSSVPMFLLMGYVSFRAGLTEGLFRAARLWLGGLPGGLAIT
                     TIGGAAAFSAVTGSSVACAAAMGRIALPEMLRYRYDPALASGAVAAAGTLGSLIPPSI
                     ILLIYGVFAEVPISRLFVAGILPGLLTAVLYAAMVVVRVRITPALAPSAREPATARQK
                     LAALGETWPVILMIVGVFGGLFGGLFTPTEAGAVGALLALVIAAAKRTLSFESFRQAV
                     SETLHSTAAIFIIAIGAQLLTRFLALSGVPEYLAEAVAVEGGGEIALVLGFSLAYLAL
                     GMFLDPIGIMLLTLPIILPVAEAVHMDLIWLGILLTKYLEIALITPPVGLNVFVVRAA
                     APDIATTTIFRGIAWFVATDLVTVALLIAFPAISLALPRLLG"
     gene            complement(4942..5445)
                     /locus_tag="VGB88_12345"
     CDS             complement(4942..5445)
                     /locus_tag="VGB88_12345"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9648285.1"
                     /translation="MSDGGARGPVERGARAAASALDWLGGIALVAMLVHVNADVLGRY
                     LFNAPLPMTTEVVSYYYMVAVVFLPLAAVEWRDGHISVELLTQLMGPRARMALLVPTA
                     VIAAGYFALLCWRTLLIAIDKYQVGEFVTGVMALDIWPTRFMVPAGCGLIALVILLRL
                     HALSRPH"
     gene            complement(5462..5938)
                     /locus_tag="VGB88_12350"
     CDS             complement(5462..5938)
                     /locus_tag="VGB88_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9648286.1"
                     /translation="MLNRLEDYVVYFEDLAVGDRFVSPARTVTEADIAAFAGLSGDYN
                     PLHTDAVAAGASAYGERIAHGLLVLAIASGLCSRTPVMKFMERSVLGLLGVECRWLKP
                     TLIGDTVHVVLEVTATRATSKPGRGLVEMRRSAVNQRGETVMESDWTLLVRARGAA"
     gene            complement(6074..6538)
                     /locus_tag="VGB88_12355"
     CDS             complement(6074..6538)
                     /locus_tag="VGB88_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648287.1"
                     /translation="MARLTFAFAALLAILAAPALGDEGSGVDYARASTGTRLLEKGDI
                     DIRMLLEASNLGGDEIEIGEITLPVSYGQGGGHLHGRMEIFYVLSGRLGHSLNGEAHV
                     LEPGMIGVVRVGDSVAHAVLSDEPVKALVIWLPAGEADRLIGEFGYSEALLD"
     gene            6695..7906
                     /locus_tag="VGB88_12360"
     CDS             6695..7906
                     /locus_tag="VGB88_12360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9648288.1"
                     /translation="MGASYERPFEGIRVADLSQGLAGPGCGLLLAAHGADVVKVEPPE
                     GDWARTLGTRHGEHAALDMAVNRGKRSIALDLKSPEGRKVALSLIARADVVIESFRPG
                     VAARLGLDYESVRACNPRALHVSISGFGQTGPYAARAATDTVGQAHSGIMSVNRDADG
                     RPVKVGFVLVDTVTALFAFQAVATSLYARADRGRLIEVSLMQSAAALIAPKIVERHLE
                     GESPRALNAPSGCYRTGDGWIAVTLIRESQYRALCQALGRPDLAGDARFADPALRADA
                     AAVLVPIVEGIIATRTTADWLDRLAAAGVMCSRVNDIGDWLEDAHVQAVDAAPLLPQE
                     PVGAVPLARIPGLPHAALANLAPEAPAIGAHGAEILADLGYSPAEIARLIAGGAVRVP
                     ARSGARAAREA"
     gene            7910..8386
                     /locus_tag="VGB88_12365"
     CDS             7910..8386
                     /locus_tag="VGB88_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9648289.1"
                     /translation="MARPPARRPAGPRFDDFRAGEVFESYRRTVTEADIATFTGLAGL
                     RLPIFLDDEYARVHSPFGSRIAPGFLTAALSAGMIESVLGSDVIAGLAMDGFRFRSPV
                     RIGDTLRAEIEIIEARPTSDGVRGVVTLAIRVLNQEDEVPLAYRASLMMHRRADPA"
     gene            complement(8411..>10881)
                     /locus_tag="VGB88_12370"
     CDS             complement(8411..>10881)
                     /locus_tag="VGB88_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9648290.1"
                     /translation="DCIVCSIDDDCVAGRSCIDIGSTMAAEGTSVRVGAYSEYSTPAN
                     HDLRQGTNTTCPAACMPIAAADCLASFNDDTGRFEGAHQTLVHGESDWETMASAQPGV
                     FSIPAALPDAGPASCQDVQPTFVSNIGELDRVILLLDRSLSMRETVAAGIDEICDNGK
                     DDDEDGQTDENNCAQSRIEYARAAARAFIDLQQNAGIELGIVLFNDTTKVQRALGPLN
                     PGNVDDFFDDVNTFNTGFDTAIGAGIDVATDEFLDDADIGNTQTILLMSDGHNNVGPD
                     PLQSAQDFKDAIDNESGIGRIFTVPVSDSADEALMSDIASDPAKMFQAPTGEELPAIY
                     AELAAIFGGDALVLPRTDGVVDGGLASYDIPVEIGAEALTIFIAGRNSRMADWDVNFA
                     LEGPASSVFEQTSCKQNADPYYCRLRVANPEPGMWSLNVSTSSTEDQSFTVLAFVENA
                     SPDCFVDLLPRVQPSETAPTLVTAGVYYRTNLEGDVGLAGTIHRPDGTAVPFSIMHDA
                     FYPAHAVGFDDYVGRGLYQVRLTCDVPEGTLPARGEPIFDGPERADVRVVPFVRHASA
                     AFYLDDGDFPVCTSPDCDNDGKPNTVDRCDVDTDHDGRPDCRDGDADGDELPDSREGR
                     DDVDLDGAPDFLDRDSDGDGIPDTYDPDPTVAAACDCSARGAIRGGPFADLLIGTARD
                     DIICGMGGNDLIFAGAGNDTICGSTGNDLIYGGPGNDLILGDGGHDILSGWLGDDLIL
                     GGPGNDVMYGDQGMDLLFGETGRDSMAGGVDDDRLFAGIGADLTAGGPDDDLQDGGPG
                     FDTQFGGPGNDVCVGGELQFGCAP"
BASE COUNT         1624 a         3884 c         3834 g         1539 t
ORIGIN      
        1 ggtcatgtcg cgcgccgccg cccggtacag cgagaagtcg ggccacaggc ggggcacgcg
       61 cgcgcaatag gcggcgtact cggcgccgaa cctgtcgtgc aggtagcgct cctcggcgac
      121 gatgatcgag gcgtagacgg cgccgaagaa tagggcgccg acggcaagcg cgacggcgtt
      181 gccgatcgcc accagcagcc ccagcatgac cagcgcgttg ccgagataga gcgggttccg
      241 cgacaccccg aacatgcccg cggtgaccag ccggtcggcg tggatcttct tgtccacccc
      301 gccgcgcttg atgtaggcga ggccgatcgt ccacagcctg aagcccgagc ccgccagcgc
      361 cagcagcagc gccgcgccgt cgttggcgag ctcgagcgcg gggtcgtcga acagcgccgg
      421 cctcagcccc agcagcaggg ccaggaagac gatcgggaag acctggttgc ggcggcggaa
      481 gaagaaccgt ccgctcgaca ggatcaggcc gtccgtgcgc gagagctcgg cgtcggccat
      541 cggctacttc accgccagca ggccgcagaa gttgtaccag cggaagaacg tctcgatcga
      601 gcggaagccg gtgtcgcgca gcaggtccag gttctcgtcg aaccggtagg ggatgagcac
      661 gttctcgagc gcctcgcgct tctgcgcgat ctcgctctcg gcgtaaccgt tgcggcgctt
      721 gaggtcgtag taatagctta tgtacagccg gttgaggtcg gagtcctcga gcgtcagctt
      781 ctcgaccagg atcaggcagc cgttgtcgcg catgccgtgc cagaccgcgt cgagcacgcg
      841 gctgcggtgc agcgggcgga cgaactgcaa ggtcaggcac atcaccacca cggaggcgtc
      901 cgagatcacg ggcaaggcgt ggatgtcggc ctcgatgaac tcgatggccc gcccgctcgt
      961 cgaggccgcc agcttttcgc gcgccttgtc gagcatgtcg cgcgagttgt cgaccccgat
     1021 caggcggact cccgcatcga gcgccgcatc gagccccgcc atggtggttc ccgaggcgca
     1081 gccgaggtcc cacacgctcg accccggggt cgcgaagtcg gccgcgatct cggccatcat
     1141 gcgctggatc tcgccgtaga acggcaccga ccggccgacc atgtcgtcga acacgcgcgc
     1201 ggtctcgcgg ccgaacttga agtcgctggg cgcgcgcgcc tccttgaaca ggcggtcgac
     1261 gttgccgggg gtcggctcgg gttcgctggc catgcgctgt ctcctgtcag cttccgccgg
     1321 ggcggccaag cccaaagccg cgtcgcgcgc caccgctcag ggcaccggct cgatccgccc
     1381 cgactcggcg gagcggaaga tcgcctccat gaccgcggca ttggcgatca tctcgccggt
     1441 cgcgatcggg taggcggcct cgcccgcggc cgcgcgcgcg aacgcctcca gattgtcgcg
     1501 caccggctcg ctgttggcca catcggtctc tgtgaccgtg ccgtccgcgc cggcgcgcag
     1561 cacggtccag cccgccggcg cctcgacatg cgccttgtcg cgaatctcga tccagccgtc
     1621 acgcccgtag accgcgaagc gcgagatgaa cggggtcgcg agcatggtcg cgacataggc
     1681 ggtggcgccc gacttgtact tgaccagcgc gcaggtcgaa tcgccgttgg cgatgtcgga
     1741 ggcgagcgtc tccgagctcg ccagcgcgaa cgcgccctcg ccgagcatgg cggtcgccag
     1801 gtcgagcacg tggatgccgg tcgcggtcat gccgccggcc ggggcgttgt ccttcgacag
     1861 ccgccagttg ccggcatcga gccgggtgaa gcggtcgtgg ctgaagttcg cttcgacctg
     1921 gaggacggtg ccgagctcgc ccgcgcgcac cgcgcgcagc atctcggcga ccggcggctc
     1981 gaaccggcgc tcgtggccga ggccgagcac cagcccggcc tcgcggcagc gggcgacgct
     2041 ctgcgcggcg gcggccttgg tgagcgccag cggcttctcg cagaacacgt gcttgccggc
     2101 cgccgcggcg gctgcgatct ggtcgtcgtg gagagcgtgc ggggtcgcca gcaccaccgc
     2161 ccggatgcgc gcgtcgccga gcgcccgggc gtaatcgggg accaccggga agccgaacgt
     2221 ctccgccgcc tgccgcgccg tcgccgggtc gggctcgacg ccgagggtga agcggatgtg
     2281 gcggctcgcg cgcgccacct cggcgagctt cttgccccac cagccgagtc cgacgatcgc
     2341 cgcctcgatc atccgcgcct cccgctcctc gccaacgcgg ccgatggtag gacatggcgc
     2401 agggcgccgc caccggtggc gcgggcaggc gcgttgcgct atggcttatg cgggagggcg
     2461 aggggaggtg cgcgggcgat gacaagaaac ctgctcgggc ggtgcgcgat cgcggcagcg
     2521 ctggcggcga cggcgattgg gtccgcctac gccgacaccg agatcaccat cagctcgtgg
     2581 gtgggaccca agcacccggt caacttcggc ggctacgagc cctatattgc cgcggtcgag
     2641 gcggccagcg gcggcgcgat ccgcttccgc ctgttcaccg gcggcgcgct gctcggcgcc
     2701 aaggcgacgc tcgccggcat ccgcgacggc atcgccgata ccggcgtgat tgcgctcacc
     2761 taccacccgg ccgagttccc gctcgcccag ctgatcgccg acctcgcgct gtggagcgac
     2821 gatgcgctgg tcgccggcgc ggcggtgacc gagttcaacc tgctccactg cccggcgtgc
     2881 ctcgacgagt tccgcgccca gggcctggtc tacaacggca cttattccac cgcgccctac
     2941 accatcatcg gccggcgccc gatcaacgtg cccgccgacc tcgccgggct caaggtccgc
     3001 accccgggct cggtgtggga ccgctgggtc cagcacatgg gcgcggtgcc ggtcaacctg
     3061 ccggcgtccg acatgtacga gagcctcgag cgcggcatca ccgacctcgt cctgcagccg
     3121 gtggcggcgt tgcgctccta cagcctgtgg gacgtcgcca ccgacgttac caacctcaac
     3181 ctcggcacct tccattcgct gtcgctgctc ggctacggcc gcgaggcgtg gcagggctac
     3241 gacgcgggcg tgcgcaagct gttgatggac aacgcgccgg tcgccctgat cggcgcctcg
     3301 cagggctatg tcgacctcga tcaggaggtg atcgccgacg ccgacgagct gaaggtggtg
     3361 atccatccgc cgggcgaggg gctcgtcgcc gcgacccgcg ccttcgccga ggctgaccgc
     3421 gccgagctcg ccgagatctc ccgcagccgg cacggcatcg ccgagcccga gccgatgatc
     3481 gagaccttcc tcggcctgct cgacaagtgg cgggcgatca tggccccgat ccgcgacgat
     3541 ccggcgaaga tggcggcggc gctcaaggcc gaggtgttcg accacctcga tcccgcgcgc
     3601 cacgggatgt gaccgcggct cagccgagca gcctgggcaa ggcgagcgag atcgccggga
     3661 aggcgatcaa cagcgccacc gtcaccaggt cggtggcgac gaaccaggcg atgccgcgga
     3721 agatggtggt ggtggcgatg tccggcgcgg cagcgcgcac gacgaacacg ttgagcccga
     3781 ccggcggcgt gatcagcgcg atctcgagat acttggtcag caggatgccg agccagatca
     3841 ggtccatgtg caccgcttcc gcgaccggca ggatgatcgg cagggtcagc agcatgatcc
     3901 cgatcgggtc gaggaacatg ccgagcgcca gataggcgag cgagaagccg agcaccagcg
     3961 cgatctcgcc gccgccctcg accgcgaccg cctcggcgag atactcgggc accccggaca
     4021 aggcgaggaa gcgggtcaag agctgcgcgc cgatcgcgat gatgaagatg gcggcggtcg
     4081 agtgcagcgt ctcggacacc gcctggcgaa acgattcgaa gctgagcgtg cgcttggcgg
     4141 cggcgatgac cagcgccaag agcgcgccca ccgcgcccgc ctcggtgggc gtgaacaggc
     4201 cgccgaacag gccgccgaag acgccgacga tcatcaggat caccggccag gtctcgccca
     4261 gcgcggcgag cttctggcgc gcggtcgcgg gctcgcgcgc gctcggcgcg agcgcgggcg
     4321 tgatgcgcac gcgcaccacc accatcgccg cgtacaggac ggcggtcagc agtccgggca
     4381 ggatgccggc gacgaacagc cggctgatcg gcacctcggc gaagacgccg tagatcaaga
     4441 ggatgatgct cggcgggatc agcgagccga gcgtgccggc ggcggcgacc gcgcccgagg
     4501 cgagcgccgg gtcgtagcgg tagcgcagca tctcgggcag cgcgatccgt cccatcgccg
     4561 ccgcgcacgc gaccgacgat ccggtcaccg cggagaacgc ggcggcgccg ccgatggtgg
     4621 tgatcgcaag cccgccgggg agcccgccga gccacagccg cgcggcccgg aacaggccct
     4681 cggtcagccc ggcgcggaag ctgacatagc ccatcagcag gaacatcggc accgagctca
     4741 gcgtccagtg ggcgacgaac tcgtacggga tctcgttcag cagcccgagc gccgcgcgct
     4801 cgccgaccag cagccacacg ccggcgatcg cgaccacgcc caaggtcacg cccaccggca
     4861 gcctggccag cagcagcacg atcatgacgg caaggccggc gaagccgatg gtgagcgggc
     4921 tcatgattgg tccgccagtc ctcagtgcgg gcgcgacagt gcgtgcaacc tgagcaggat
     4981 caccagcgca atcaggccgc agccggccgg caccatgaac cgggtcggcc agatgtcgag
     5041 cgccatcacc ccggtgacga actcgccgac ctggtacttg tcgatcgcga tcagcagcgt
     5101 ccgccagcac agcagggcga agtagccggc ggcgatgacc gcggtcggga ccagcagcgc
     5161 catgcgcgcg cggggcccca tcagctgggt cagcagctcg acgctgatat ggccgtcgcg
     5221 ccactcgacc gcggccagcg gcaggaacac caccgccacc atgtagtagt aggacaccac
     5281 ctcggtggtc atcggcagcg gcgcgttgaa cagatagcgg ccgagcacgt cggcattcac
     5341 gtggaccagc atcgccacca gggcgattcc gccgagccag tcgagcgcgc tcgccgcggc
     5401 gcgcgcgccg cgctcgaccg ggccccgggc gccgccgtcg ctcacgcttc cgcgcctccg
     5461 ctcacgccgc cccgcgggcg cgtaccagca gcgtccagtc gctttccatc accgtctcgc
     5521 cgcgctggtt gaccgccgag cggcgcatct cgaccagccc gcgccccggc ttgctggttg
     5581 cgcgcgtggc cgtcacctcg agcaccacgt ggacggtatc gccgatcagg gtcggcttga
     5641 gccaccggca ctcgacgccg agcagcccga ggaccgagcg ctccatgaac ttcatcaccg
     5701 gcgtgcgcga gcacagcccc gaggcgatcg ccagcaccaa gagcccgtgg gcgatgcgct
     5761 cgccataggc gctggcgccg gcggcgacgg cgtcggtgtg cagcgggttg tagtcgcccg
     5821 acaggccggc gaaggcggcg atgtcggcct cggtcaccgt gcgcgccggc gagacgaacc
     5881 ggtcgccgac cgccaggtcc tcgaaataga cgacatagtc ttcgagccgg ttcagcatcg
     5941 ccgcccccct gcttgcgcac cgttggcccc gaggaccggc gccgacctcc ggaacccgcc
     6001 gccgagcctc cgcctcggcc gcggaccgcg cgccgtccca cgacgaaatg gcgtgtcctt
     6061 agcctggcaa cggctagtcg aggagagctt ccgagtagcc gaactccccg atcagacgat
     6121 ccgcctcgcc ggccgggagc cagatcacca gcgccttgac cggctcgtcg gacagcaccg
     6181 cgtgggccac cgagtcgccg acgcgaacga cgccgatcat gccgggctcg agcacgtgcg
     6241 cctcgccgtt gaggctgtgg ccgagccgcc cggagaggac gtagaagatc tccatgcggc
     6301 cgtgcagatg gccgccgccc tggccatagc tgacgggcaa ggtgatctcg ccgatctcga
     6361 tctcgtcgcc gccgagattc gacgcctcga gcagcatgcg gatgtcgata tcgcctttct
     6421 cgagcagccg ggtgccggtg cttgcgcgcg cgtaatcgac gcccgagccc tcgtcgccga
     6481 gcgccggcgc agccaggatc gccaacaggg ccgcgaatgc aaacgtcagg cgcgccattt
     6541 tcgtttcctc ccaaatccgg tgccgccatc ccaccgttcc gatcctgtct caaatcaggc
     6601 cgccgcgcaa cggaggcccg gtcggcgaac gcgcgcgtcc ttgccgcggg cgtgcgccca
     6661 gcccacaatg ccgccggagg atgacggcag ggcgatgggc gcgagctacg agcggccgtt
     6721 cgagggcatc cgcgtggccg acctcagcca gggcttggcc gggccgggct gcgggctgct
     6781 gctcgccgcc cacggcgcgg acgtggtcaa ggtcgaaccg cccgagggcg actgggcgcg
     6841 caccttgggc acccgccacg gcgagcacgc ggcgctcgac atggcggtca accgcggcaa
     6901 gcgcagcatc gccctcgacc tcaagtcgcc cgagggccgc aaggtggcgc tcagcctgat
     6961 cgcgcgcgcg gacgtggtga tcgagagctt ccgccccggc gttgcggcgc gcctcgggct
     7021 cgactacgag tccgtgcggg cgtgcaatcc gcgcgcgctg cacgtctcga tcagcggctt
     7081 cggccagacc ggtccctacg ccgcgcgcgc cgccaccgac acggtcggcc aggcgcattc
     7141 gggaatcatg tcggtcaacc gcgacgccga cgggcgcccg gtcaaggtcg gcttcgtgct
     7201 ggtcgatacg gtgaccgcgc tgttcgcgtt ccaggcggtc gcgacctcgc tctacgcgcg
     7261 cgcggaccgg ggccggctga tcgaggtcag cctgatgcag tcggcggccg ccctgatcgc
     7321 gcccaagatc gtcgagcgcc atctcgaggg cgagagcccg cgcgcgctca acgcacccag
     7381 cggctgctac cgcaccgggg acggctggat cgcggtcacc ttgattcgcg agtcccagta
     7441 tcgggcgctg tgccaggcgc tcggccgacc cgacctcgcc ggcgatgcgc gcttcgccga
     7501 tcccgcgctg cgcgcggacg ccgctgcggt cctggtgccg atcgtcgagg gcatcatcgc
     7561 cacgcgcacc accgccgact ggctcgaccg cctggccgcg gccggcgtga tgtgcagccg
     7621 ggtcaacgac atcggcgact ggctcgagga tgcgcatgta caggcggtgg atgcggcgcc
     7681 gctgctgccc caggagccgg tcggcgcggt gccgctcgcg cgcatcccgg ggctgccgca
     7741 cgccgcgctc gccaacctcg cgcccgaggc gccggcgatc ggcgcacacg gggccgagat
     7801 tctcgccgac ctcggctatt cgcccgccga gatcgcgcgc ctgatcgcgg gcggcgcggt
     7861 gcgcgtcccg gcgcgttcgg gcgcgcgtgc ggcgcgcgag gcttagccga tggcgcgccc
     7921 gcccgcccgg cgaccggccg ggcctcggtt cgacgacttc cgggccggcg aggtgttcga
     7981 gagctaccgg cgcacggtga ccgaagccga catcgccacc ttcaccggcc tcgccgggct
     8041 caggctgccg atcttcctcg acgacgagta tgcgcgcgtg cactcgccgt tcggctcgcg
     8101 catcgcgccc gggttcctga ccgcggcgct gtcggccggg atgatcgaga gcgtgctcgg
     8161 cagcgacgtg atcgccggcc tcgccatgga cggcttccgc ttccggtcgc cggtgcgcat
     8221 cggcgacacc ttgcgcgccg agatcgagat catcgaggcg cgcccgacct ccgacggcgt
     8281 gcgcggcgtc gtcaccctcg ccatccgcgt gctcaaccaa gaggacgagg tgccgctcgc
     8341 ctaccgggcg agcctgatga tgcaccggcg cgccgacccg gcataagcgc gctccgccgc
     8401 cgaccgcagc ttaaggcgcg cagccgaact ggagctcgcc gccgacgcag acgtcgttgc
     8461 cgggaccgcc gaactgggta tcgaaccccg ggccgccgtc ctgcaggtcg tcgtcggggc
     8521 cgcccgcggt gaggtcggcg ccgatgccgg cgaacaggcg gtcgtcgtcg accccgccgg
     8581 ccatgctgtc gcgtccggtc tcgccgaaca gcaggtccat gccctggtcg ccgtacatca
     8641 cgtcgttgcc ggggccgccg aggatcaggt cgtcgccgag ccagccggac aggatgtcgt
     8701 ggccgccgtc gcccaggatc aggtcgttgc ccgggccgcc gtagatcagg tcgttgccgg
     8761 tggagccgca gatcgtgtcg ttgccggcgc cggcgaagat caggtcgttg ccgcccatgc
     8821 cgcagatgat gtcgtcgcgc gccgtgccga tcaggaggtc ggcgaacggg ccgccgcgga
     8881 tcgccccgcg tgcgctgcaa tcgcaggccg cggccacggt cgggtcgggg tcgtaggtgt
     8941 cggggatgcc gtcgccgtcg gaatcgcggt cgaggaagtc gggcgccccg tccaaatcga
     9001 catcgtcacg tccttcccgc gagtccggca actcgtcgcc gtcggcgtcg ccgtcgcggc
     9061 agtccgggcg cccgtcgtgg tcggtgtcca cgtcgcagcg gtcgacggtg ttgggcttgc
     9121 cgtcgttgtc gcagtcggga ctggtgcaga cggggaagtc gccgtcatcg aggtagaacg
     9181 ccgcgctcgc atggcgcacg aacggcacca cgcggacgtc ggcgcgctcc gggccgtcga
     9241 aaatcggctc gccccgcgcc ggcagcgttc cctcgggcac gtcgcaggtg agccgcacct
     9301 ggtagaggcc gcggccgacg taatcgtcga acccgaccgc atgagcggga tagaacgcgt
     9361 catgcatgat cgagaacggg acggccgtgc catcggggcg atggatggtt cccgcgaggc
     9421 ccacgtctcc ctcaagattg gtgcggtagt acacgcccgc ggtcaccagg gtcggggcgg
     9481 tctccgacgg ctggactcgc ggcaacaggt cgacgaagca gtccgggctg gcgttctcga
     9541 cgaacgccag caccgtgaac gactggtctt cggtggagga tgtcgagacg ttcaagctcc
     9601 acatccccgg ctccgggttc gcgacgcgca ggcggcagta gtaggggtcg gcattctgct
     9661 tgcacgacgt ctgctcgaac accgaggacg ccggaccctc gagcgcgaag ttcacgtccc
     9721 agtccgccat gcgcgaattg cgcccggcga tgaagatggt cagcgcctcg gcgccgatct
     9781 cgaccggtat gtcgtagctc gcgaggcccc catccaccac gccgtcggtg cgcggcagca
     9841 ccagcgcgtc gccgccgaag atcgcggcaa gctcggcata gatcgccggc agctcctcgc
     9901 cggtcggcgc ctggaacatc ttggccgggt ccgaggcgat gtcggacatc agcgcctcgt
     9961 ccgcggagtc gctgaccggg accgtgaaga tgcgcccgat gccgctctcg ttgtcgatcg
    10021 cgtccttgaa gtcctgggcg ctctgcaacg ggtcgggccc gacattgttg tggccgtcgg
    10081 acatcagcag gatggtctgg gtgttgccga tgtcggcatc gtccaggaac tcgtcggtcg
    10141 cgacgtcgat gccggcgccg atcgcggtgt cgaagccggt gttgaaggtg ttcacgtcgt
    10201 cgaagaaatc gtcgacgttg cccgggttca gcgggccgag cgcgcgctgc accttggtgg
    10261 tgtcgttgaa cagcacgatg ccgagctcga tgccggcgtt ctgctgcagg tcgatgaacg
    10321 cgcgcgccgc ggcccgcgcg tactcgatgc ggctctgggc gcagttgttc tcgtcggtct
    10381 ggccatcctc gtcgtcgtcc ttgccgttgt cgcagatctc gtcgatgccg gcggccacgg
    10441 tttcgcgcat gctgagcgag cggtcgagca gcaggatgac cctgtccagc tcgccgatgt
    10501 tgctgacgaa ggtcggctgc acgtcctggc acgaggccgg tccggcgtca ggcaacgccg
    10561 ccggtatgga gaagactcca ggctgggcgc tcgccatcgt ctcccaatcg gactcgccgt
    10621 ggaccagcgt ctggtgcgcg ccctcgaaac gtccggtgtc gtcgttgaag ctcgccaggc
    10681 agtcggcggc ggcgatcggc atgcaggcgg cgggacaggt cgtgttggtc ccctgcctca
    10741 agtcgtggtt ggccggcgtg ctgtactcgc tgtaggcgcc gacccgcacc gaggtcccct
    10801 cggcggccat cgtgctgccg atgtcgatgc acgaccgtcc cgccacgcag tcgtcgtcga
    10861 tgctgcacac gatgcagtcc g
//
LOCUS       DASWHU010000330         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_201045, whole genome shotgun sequence.
ACCESSION   DASWHU010000330 DASWHU010000000
VERSION     DASWHU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201045"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VGB88_12375"
     CDS             <1..938
                     /locus_tag="VGB88_12375"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEX9648291.1"
                     /translation="LFDIALYIHRGLGDAMVDGTPAVPRASPNHAEGDRMTFDIGRAV
                     LAMTAALGGLVAGGAAHALDLLPHRALYTLSLAAVRGSSDISSLDGRMALEWADACDG
                     WTVNQKVLMTLGNRNGPAVTNEFSFSSFESKNGDNLRFTMRSSADGEPYDEFVGTAQR
                     SAQGGKVEFSQPEGLTLDLPAGTIFPSEHLALLVEGALAGKTWIAESVFSGTGPESLH
                     QVTAFIGPEIPVGSRPLVPAGTGDATLLALGELRSWPVSMAYFPLRRKDAEPEFEVSY
                     RLVENGVAANLVLDYGEFAMRAILSKLEFLDPPDC"
     gene            complement(946..>2014)
                     /locus_tag="VGB88_12380"
     CDS             complement(946..>2014)
                     /locus_tag="VGB88_12380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9648292.1"
                     /translation="ELNQLAIAHLDCDAFYAAVEKRDNPALQDKPVIVGGGRRGVVST
                     ACYIARLYGVGSAMPMFKALEACPDAVVIHPDMRKYQAVGREVRSLMAETAARVQPVS
                     IDEAFLDLSGAGEGAEAPAERLVRLARRIEAEIGITASIGLSYNKFLAKLASDLDKPR
                     GFAVVGRAEAQAFLAALPVRKLWGVGPAFAARLQRDGINLIGQLQERDEAWLVRRYGS
                     IGHRLAGFSRGEDDRQVQAREVAKSVSAETTLDADSGEAAVLAARLERLCARVADRLR
                     RGERAAQTVVLKLKTRDFRLRTRSATLAAPTQRAEVLLRAADALLRREIDGTKFRLIG
                     VAGANLTAGAGADPPDMLDAG"
BASE COUNT          282 a          719 c          705 g          308 t
ORIGIN      
        1 ttttgttcga catcgctctc tatattcatc gcgggctggg ggacgcgatg gtcgatggca
       61 cgcccgccgt gccgcgcgca agcccaaacc acgcggaagg cgaccgaatg accttcgaca
      121 tcggccgggc cgtgcttgcg atgacggccg cgcttggcgg cctggtcgcg ggcggcgccg
      181 cgcacgcgct cgacctgctc ccccatcgcg cgctctacac cctcagcctc gccgcggtca
      241 ggggctcgag cgacatctcg tctctcgacg ggcgcatggc gctcgaatgg gcggacgcct
      301 gcgacggctg gacggtcaac cagaaggtgc tgatgactct cggcaaccgc aatggaccgg
      361 cggtaaccaa cgagttcagc ttctccagct tcgagagtaa gaacggcgat aacctgcgct
      421 tcaccatgcg ctcttccgcc gatggcgagc cgtacgacga gtttgtcggc acggcgcagc
      481 gctcggcgca gggcggcaag gtggagttct cgcagcccga aggcctcacg ctcgacctgc
      541 cggccggcac gatctttccg tccgagcacc tggcgctgtt ggtcgagggt gcgctcgccg
      601 gcaagacctg gatcgccgag tcggtgttca gcggcaccgg gccggagagc ctgcaccagg
      661 tcaccgcctt catcggcccg gagatcccgg tcggcagcag gccgttggtg ccggcgggca
      721 ctggcgacgc cacgctgctg gcgttgggcg agctgcgctc atggccggtg tcgatggcct
      781 acttcccgct caggcgcaag gatgccgagc ccgagttcga ggtctcctac cggctggtcg
      841 agaacggcgt ggccgccaac ctggtgctcg attacggcga gttcgcgatg cgggcgatcc
      901 tgtccaagct cgagttcctc gacccgcccg actgctgaca ggctctcacc cggcatccag
      961 catgtccggc gggtcggcgc cggcgccggc ggtgaggttg gcgccggcga cgccgatcag
     1021 ccggaacttg gtgccgtcga tctcgcgcct gagcagcgcg tcggcggcgc gcagcagcac
     1081 ctcggcgcgc tgggttggcg cggccagtgt tgctgagcgg gtgcgcagcc ggaagtcccg
     1141 cgtcttgagc ttcagcacca ccgtctgcgc cgcgcgctcg ccccgccgca ggcgatcggc
     1201 gacgcgcgcg caaagtcttt cgagccgggc ggcgagcacc gccgcctcgc cgctgtcggc
     1261 gtcgagcgtg gtctccgccg acacgctctt ggcgacctcg cgcgcctgca cctggcgatc
     1321 gtcctcgccg cgggaaaaac cggcgagccg atggccgatg ctgccgtacc ggcgcaccag
     1381 ccacgcctcg tcgcgttcct gcagctggcc gatcaggttg atgccgtcgc gctgcaggcg
     1441 ggcggcgaac gccgggccca cgccccacag cttgcgcacc ggcagcgcgg cgaggaaagc
     1501 ctgcgcctcg gcgcgcccga ccacggcgaa gccgcgcggc ttgtcgaggt cggaggcgag
     1561 cttggccagg aacttgttgt agctgaggcc gatcgaggcg gtgatcccga tctcggcctc
     1621 gatccgccgg gcgagccgga ccaggcgttc ggcgggcgct tcggcgccct cgccggcccc
     1681 ggacaggtcg aggaacgcct cgtcgatgga caccggctgc acccgcgccg cggtctcggc
     1741 catcagcgac cgcacctcgc gcccgaccgc ctggtacttg cgcatgtcgg gatggatcac
     1801 gaccgcgtcg gggcatgcct cgagcgcctt gaacatcggc atcgccgagc ccacgccgta
     1861 caggcgcgcg atgtagcagg cggtgctgac caccccgcgc cggccgccgc cgacgatcac
     1921 cggcttgtcc tgcaaggccg ggttgtcgcg cttctcgacc gcggcataga acgcgtcgca
     1981 gtcgagatgg gcgatggcaa gctggttcag ctcg
//
LOCUS       DASWHU010000331         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1309521, whole genome shotgun sequence.
ACCESSION   DASWHU010000331 DASWHU010000000
VERSION     DASWHU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309521"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VGB88_12385"
     CDS             complement(<1..1359)
                     /locus_tag="VGB88_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648293.1"
                     /translation="MDPLPGDAGGGVMATLALIDSLPALAHVRAAHPGAVAVTDNPLL
                     AADPRSAGAVDNADALIDQPACFALGRLAIEVANALDAHLRARGIEARFGLVPGHVRL
                     AGLTSRLVAGVLYRASLLARLVADRRPGRLVLALHDAPAWDPAQPLVPPRFGAPYAGL
                     AARGFVDGVEVVTEPVPVPVPARVNDTGILDLGRRIAPVPAAALIHEGLARLGLESSV
                     GGATLAVGAGNEALDETLLWLWLRGYRLVRLGSPARAAAEVRTDPAAPPPTGALDAEI
                     ADWLAANLAATDGLGTGQVRAVAHLISGHVAAGLDHLGRQAEAVRRHLDSPWPGARRP
                     RALLTNGLIGPLGAQIYGLLSERGVPVIDFEHGVTTSLSALSQARIDASEAAACDLLL
                     VCAEDAARAFRKANRADAPVVHTIGLADQTRRLLRPRWQRRLARRRLGLGADAAVVMH
                     VST"
     gene            complement(1323..>1794)
                     /locus_tag="VGB88_12390"
     CDS             complement(1323..>1794)
                     /locus_tag="VGB88_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648294.1"
                     /translation="DALDRVEGALRVTVALGASAPGLESVRAKRRARGDAGLVTDAAD
                     LIGLYEASDIAIGAPGVSFLERLCCGLPSVLVAQGPRQRPSLDWAARAGSALVAEADV
                     AAIARAVQRLLDDDFLWRRLRARGMRLIDGRGAGRLAARLHEAWTRFPVTPAAG"
BASE COUNT          246 a          681 c          684 g          183 t
ORIGIN      
        1 ggtcgagacg tgcatgacca ccgccgcgtc ggccccgagg ccgagccgcc ggcgggccaa
       61 gcggcgctgc cagcgcggcc tgagcaggcg gcgggtctgg tcggcgaggc cgatcgtgtg
      121 cacgaccggc gcgtccgcgc ggttcgcctt gcggaacgcg cgcgcggcgt cctcggcgca
      181 caccagcaac aggtcgcagg ccgcggcctc ggaggcgtcg atgcgcgcct gggacagcgc
      241 cgacagcgat gttgtgacgc cgtgctcgaa atcgatcacc ggcacgccgc gctcgctgag
      301 caggccatag atctgggcgc cgagcggacc gatcaggccg ttggtgagca gcgcccgcgg
      361 ccggcgcgcg cccggccatg gcgagtcgag atgccggcgc accgcttcgg cctggcggcc
      421 gagatggtcg aggccggcgg cgacatggcc cgagatcaag tgcgccaccg cccgtacctg
      481 cccggtgccg agcccatccg tcgcggcgag gttcgccgcc agccagtcgg cgatctcggc
      541 gtcgagtgcg cccgtgggcg gcggtgccgc cgggtcggtg cgcacctcgg ccgcggcgcg
      601 tgccggcgac ccgagcctga ccaaccgata gccgcgcagc caaagccaga gcagagtctc
      661 atcgagcgcc tcgttgccgg cgccgacggc gagcgttgcg ccgccgaccg acgattcgag
      721 tccgagccgc gctagcccct cgtggatgag cgccgccgcc ggcaccggcg cgatgcgccg
      781 gccgagatcg aggatgccgg tgtcgttgac ccgcgccggc accggcaccg gcaccggctc
      841 ggtgacgacc tcgacgccgt cgacgaagcc gcgcgcagcg agcccggcat agggagcgcc
      901 gaagcgcggc ggcaccagcg gctgcgccgg gtcccaggcc ggcgcgtcgt gaagcgccag
      961 caccagccgc ccgggcctgc ggtcggccac cagccgggcg agcaagctcg cccggtagag
     1021 cacgcccgct accagccggg aggtgagccc ggccaacctg acatggcccg gcaccaggcc
     1081 gaagcgcgcc tcgatgccgc gcgcgcgcag atgcgcatcg agcgcgttcg cgacctcgat
     1141 cgccagccgg ccaagggcga agcacgccgg ctggtcgatc agcgcatcgg cgttgtcgac
     1201 cgcaccggcc gagcgcgggt cggcggcgag cagcgggttg tcggtgaccg cgacggcgcc
     1261 ggggtgggcc gcgcggacgt gggcgagcgc gggcaggctg tcgatcaggg cgagggtggc
     1321 catcaccccg ccgccggcgt caccgggaag cgggtccacg cctcgtgcag ccgcgcggcg
     1381 agccgccccg cgccgcgtcc gtcgatgagg cgcatgccgc gcgcgcgcag ccgccgccac
     1441 aagaagtcgt cgtcgagcag gcgctgcacc gcgcgcgcga tcgccgccac atcggcctcg
     1501 gcgaccagcg cgctgcccgc gcgcgccgcc cagtcgaggc tcggccgctg acgcgggcct
     1561 tgcgccacca gcaccgacgg caggccgcag cacaaccgct cgagaaagga gacgcccggc
     1621 gcgccgatcg cgatgtcact cgcttcgtaa aggccgatca ggtcggcggc gtcggtgacc
     1681 aggccggcgt cgccgcgcgc gcggcgcttt gcgcgcaccg actcgaggcc gggcgcactc
     1741 gctccgagcg cgacggtgac tcgcagcgcg ccctcgacgc ggtcgagagc atcg
//
LOCUS       DASWHU010000332        30359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_944811, whole genome shotgun sequence.
ACCESSION   DASWHU010000332 DASWHU010000000
VERSION     DASWHU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 30359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30359
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944811"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VGB88_12395"
     CDS             complement(<1..504)
                     /locus_tag="VGB88_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648295.1"
                     /translation="MSFKHLIGFAAAAALVAAAAGSASAQYYEGMDAGFPAPTGDAAF
                     VPAGSKLMRVFDGGCILTEGVAAGHDGYMYFSDITFSKFCKDPSGMYVQAGNIWRYDP
                     KSGESTIFRSPSGMSNGLKFDRDGNMLAALGADFGGRMLIKTDMATGKTYILSGLYNG
                     RPYNALND"
     gene            complement(705..1991)
                     /locus_tag="VGB88_12400"
     CDS             complement(705..1991)
                     /locus_tag="VGB88_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011456203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9648296.1"
                     /translation="MVSAIAVLLVMGLPLAFVTGFVAMAFALGEFGMPGLFLIESRIF
                     SFVREFVLVAVPMFILMASVLERSGVARDLYRAMHIWAGGLRGGLAIQTMVVAVLMAA
                     MTGIIGGEIILLGLVALPQMLRLNYDRKLAIGTICAGGSLGTMIPPSIVLIIYGLTAG
                     VDIGELFVATIIPGLLLATIYVAYIALRCQINPELGPPAPVEERNLPRREKLRLIGGL
                     VLPLGVAGAVLGSIYLGIASVSEAAGMGAVGAIVSAAVRRELSWPMLREALFQTMNTC
                     GMLLWITFGATALIGVYNLMGGNAFVKGLITGLPFGPLGIVVVMMAVLVVLGMFMDWI
                     GICLLTMPIFVPAVVALGYDPVWFGILFCMNMQISYLTPPFGPAAFYLKGVAPPGIEL
                     ANIYNAMWPFIGLQAVGLAMVLLVPDIALWLPAAIR"
     gene            complement(2015..2533)
                     /locus_tag="VGB88_12405"
     CDS             complement(2015..2533)
                     /locus_tag="VGB88_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9648297.1"
                     /translation="MVERPRAEPAVDRFARRTGELFSYLFLASMLIIAFEVVSRYAFG
                     APTVWAHDTTTTLSAVGFVVSGVYTLQRRAHIRITLLYDRMPAAARRALDVVNELIML
                     AFVGLLGWQSSLDAWKSISIMETAGTASRLPLPPIVKSAMVFACAAMFALGIGQLFRD
                     LSRRSRDREPPP"
     gene            complement(2543..3595)
                     /locus_tag="VGB88_12410"
     CDS             complement(2543..3595)
                     /locus_tag="VGB88_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9648298.1"
                     /translation="MPRLARAVTAIRRAGCALALVAISASAVTIPARAADTEWTFQAA
                     TSTGEIAFQIMQDFTDGIETLTGGRLKVRLLPVGAVVQYSETLDAVGAGLLDGHITAT
                     VYFSGKDAAFALLGDLIAAYDHPDQMLMFLHKGGGDQLLADLHAGYGVHYIGGATTGK
                     EAFVSKIPLRGVDDFRGVKLRAPEGMAQDIFQRIGAAPVNLPASEVFTALERGVVDAA
                     DWATFSMNHQLGFHRIARYPIYPGIHSMPVIEVSVNQARWDALAPDVQAILEVAVRDF
                     ARDMTRRLELQDLADVAADRGAGVEIVDWPASERQKLRNIAIEVWRDWAKRSPGAERA
                     YQAQIKFLRTIGLLTD"
     gene            complement(3607..4641)
                     /locus_tag="VGB88_12415"
     CDS             complement(3607..4641)
                     /locus_tag="VGB88_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9648299.1"
                     /translation="MSRRVGRWIAVALAAALALPGAPHAEPQFSWKMQSLWQAGSINQ
                     KVFEAFCARVGEITGGRLVIEPLPVGTVVAYNETLDAVAAGLLDSMHGGTGYFAGKDP
                     AFSLLADLNGGYDNVRQLHMWLDYGGGIELGRELFAQYGSYYVGAVSWGHESIPSKKP
                     IRSLADFKGVKIRAPEGMAQEIFKRIGAAPVNIPGAEVYTALERGVVEAADWGTLGMN
                     DDLGYHAIAPYPLYPGFHSAPMADVAVNTDRWNELPADIKALVEMAVRDFSRDMVEEI
                     ALQDAAVAAHAAEKGIELVSWSADERRKFREVAVEVWAEYAARSPMAKRIYDSHIAFL
                     KRLGLLGASD"
     gene            complement(4757..5590)
                     /locus_tag="VGB88_12420"
     CDS             complement(4757..5590)
                     /locus_tag="VGB88_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9648300.1"
                     /translation="MSRLLTDAQIEAYRRDGYVFPVPIMPEAAAGKLRARLEAAEAAK
                     GGKFEGTLRFKPHLLYTFLADLVRDETVLDAVEDVIGPNILCWGSSFFTKDARDPRYV
                     SWHQDSTYWGLSVPDVATAWVALSPSVPENGCMRVIPGTHTLGQLPHVDTNAKDNMLT
                     RGQVVDYPVDEGRAVDVILRPGEMSLHHVRLIHGSNPNPSDDRRIGFAVRYIAPHVRQ
                     LNGPHDSAVLVRGEDDFGYYEREPKPASDFDPIAVAAHTRAAELHGALLYQGSERAPF
                     S"
     gene            5766..5996
                     /locus_tag="VGB88_12425"
     CDS             5766..5996
                     /locus_tag="VGB88_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9648301.1"
                     /translation="MLAKLTSKNQLTLPKAVLEAVAKADYFDVVAEDGRIVLTPVRVA
                     RAEAVRAKLRELGVDPKDVAAAVGWARDAKSR"
     gene            5993..6445
                     /locus_tag="VGB88_12430"
     CDS             5993..6445
                     /locus_tag="VGB88_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX9648302.1"
                     /translation="MRVVLDTNVLVSALLFSRGQVSWLRPAWQDGGLRPLVSKATATE
                     LLRVLQYPKFRLTTAERDDLLADVLPFCETVTLDDPPANVPSCRDPHDTKFLELALAG
                     GADALVTGDADLLALAPAFAVPIVTPAQLKERLRAARRRDSPGCSDGS"
     gene            6756..7865
                     /locus_tag="VGB88_12435"
     CDS             6756..7865
                     /locus_tag="VGB88_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9648303.1"
                     /translation="MKITDLTITLFAWTGIPPTSYARHTGRFAGESRLGLVAVATDQG
                     VTGHAVIGAAAHGADQDAGTVVKYLKPLLIGEDPLDRARLYDRIGARRRYVPPRCLGA
                     VDVALWDIAGKVAALPVHRLLGSFRHAVPAYASSAVLEAPAAYAEEAVRFKEAGWRAY
                     KIHPFGRWREDIEICRAVRRAVGDDYTLMLDSTWNYRYEHALKVGRAIEELGFYWYED
                     PLADDDIYNCVKLKEKLDIPLMATEYAPGGFTAYAPWLLLRATDYLRGDVAVKGGITA
                     MVKSAHLAEAFALNYEVHHGGNSHNNWANLHVIAAIPNTEFFEVLLPAAAQKYGVVDD
                     LEVDRDGLVHVPDRPGIGAEIDFELIERTKLGVLA"
     gene            8113..10287
                     /locus_tag="VGB88_12440"
     CDS             8113..10287
                     /locus_tag="VGB88_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9648304.1"
                     /translation="MEAKVVWLFVFVAVYWAYCIFWGVKGALSARTASDYFIAGRGIS
                     VWVFVLAATATSFSGWTFMGHPGLVYRDGFPYAYASFYAITIPFTGVLFLKRQWMIGK
                     RFGFVTPGEMLSDYFRSDVIRILVVIVALSFSIPYLGVQLRASGFLFNVLTDGMLSVD
                     VGMWLLSAVVIIYVASGGLRAVAYVDTVQCILLSAGIVIIGLITLSYVGGLGVLIDGI
                     GALTQIDAKTTPDGYSHYVAIPGVIQFVDNSAKAQGGVWTGIMVLTYMFALMGIQSAP
                     AFSMWAFSNRSPQPFAPQQVWASSFGIGLILFVFTAIQGLGSHQLGTDLAFMRQFPEL
                     TSESASVLQTGVIESQYRGALDPLDRSETRDVVAAGIEQFKATGTVPTFADIAAGGGP
                     AAEFARRVVSASAGTNFNPGASPVLLDLVADLKQPDMLVPQLIRLMQDVAPWMVGLLS
                     VCALAAMQSTGAAYMSTAGGMLTRDLLKHYLMPSASHKLQKFWGRVGVVVIVMAALIV
                     ASASSDALVLLGGLAVAYGFQMWPALIAVCWWPWLTREGVTLGLIAGLIGVTLTDSLG
                     PDWFGIEAWGRWPLTIHSAGWGMLFNIGVAILVSLVTQRDSDLAHRMTFHSFLREHAS
                     LPASKRNLIPRAWAITLIWFVFAIGPGAVIGNYIFGSPTDPSSWLFAIPSIWAWQLLW
                     WALGVFMMWYLAYKMELSTVPSREVEALHEDIGDIQLALDKP"
     gene            10295..10537
                     /locus_tag="VGB88_12445"
     CDS             10295..10537
                     /locus_tag="VGB88_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648305.1"
                     /translation="MTGAADSGYLLWVVALAAALWWPTSRLVWVLSVRRLERKLGRRL
                     GEGELAGQKRRAWLIAAALSLIFSALFNLRLIDRGG"
     gene            10570..11154
                     /locus_tag="VGB88_12450"
     CDS             10570..11154
                     /locus_tag="VGB88_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648306.1"
                     /translation="MTADARSDARWKGLAIALAIGCAAWFLYDTLIAARDPALDAQSA
                     ADRQFEDGRYERALALYRERLAVEPANAYALRGAALSLMQLGRDADALAAFADAIAAD
                     PGFAGAYANRGILHDRMGDWQAAVADYERALALDPEVADGPHWLTLFLRNQPERPPGV
                     ADRLAYLRQELAKPEGERVLRMPELDQAQRPYQQ"
     gene            11260..11682
                     /locus_tag="VGB88_12455"
     CDS             11260..11682
                     /locus_tag="VGB88_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX9648307.1"
                     /translation="MRLVLDTDVLVAAMRSPRGASAALLRAVREGSATLLLSVPLALE
                     YEAVCRQRQHRLAAALSDKEVTTFLDAVIAMAEPVEMHFLWRPQLQDPADEMVLETAV
                     NGVAEALVTFNSRDFGAAPSRFGIEILLPRDALTRIRR"
     gene            11679..11990
                     /locus_tag="VGB88_12460"
     CDS             11679..11990
                     /locus_tag="VGB88_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HEX9648308.1"
                     /translation="MTKAKTYSLRLPRSLKEAVAELSREEGTSINQFVATAVAEKVSA
                     LSTARYFAQRRARADLAAFDRLMGRSAGETPREGDEIAEHGPGRVRNRKVSPGCRRKR
                     S"
     gene            12119..12376
                     /locus_tag="VGB88_12465"
     CDS             12119..12376
                     /locus_tag="VGB88_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9648309.1"
                     /translation="MPNLIIRIGQALRLASRLARLTGETLTEAVAEALRERLEREERR
                     RQRTGIAARLMEIGRRCSAVPMRDARSREEILGYDERGLPQ"
     gene            complement(12540..12713)
                     /locus_tag="VGB88_12470"
     CDS             complement(12540..12713)
                     /locus_tag="VGB88_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648310.1"
                     /translation="MPGVFDVVFLLITGAVAWHALTWRDEHGKSDWVRLLFGCIALLF
                     FLRVLLVDVLGLV"
     gene            complement(12752..13162)
                     /locus_tag="VGB88_12475"
     CDS             complement(12752..13162)
                     /locus_tag="VGB88_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648311.1"
                     /translation="MFSLDPDLSEDVTNVLDSAGLLEVSEFEVFRMAYASWFGEPTGD
                     SDRRVLDRYFFDYLYRDKVPPWARAFTREVVRRADANCLDLADYGIVHPPPTPTMIYL
                     GIRYAIWAAFTIAFIVATAHMVNGPEGCILPPCY"
     gene            13293..14162
                     /locus_tag="VGB88_12480"
     CDS             13293..14162
                     /locus_tag="VGB88_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HEX9648312.1"
                     /translation="MARSMVFAALLACLAVLGARGAGAACPSAGEVDARVAAYLAAQP
                     VASYGAGLSIADAYCGQDLYVARLEAALGPRIGYKVGFTGKASQERFGVTEPARGVLF
                     APMILESGAELPADYGVRPLYEADLIVTIADASIMDATTPLEAAAALGAVVPFIELPD
                     IVVPEGETLDAANIIALNVMPRYGVVGPGIPVDADEAFVAAFADMEAIVIDETGAEIE
                     RSRGSALLGNPLNAMLWLVGNLKASGLALKPGDMISLGSLGKLYPTAPGKTITMRYQG
                     LPGGTSEVSVRFR"
     gene            14198..15655
                     /locus_tag="VGB88_12485"
     CDS             14198..15655
                     /locus_tag="VGB88_12485"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX9648313.1"
                     /translation="MASQTAVFTRVVGDHMRAAPPTVPAGTPIADALARLVAAKATAL
                     IVTDQGGRIAGIVTEQDVARRIACRAGRTAPIEQAMTKPVATVRSDDPLYAAIGQMRK
                     RALRHLPVVDAAGAPVGMIELYAALQVAAAQMVDQIDRLTHDATEAGLKAVKGEQVAV
                     AEQLFADGVAGGEVLALISQINCDLYRRVVERSLGEMAACGAGAPPVEFAVLVMGSGG
                     RGESAIGADQDNGFVLADYDDSDHDRIDAFFIALAESMTAMLDRIGLPLCPGGVMATN
                     PLWRKSLGQWRHQIGFWIRRHDEGLVRLADIFFDFRCVYGREDLARALREAVTERMRT
                     AHAFLRTMSTVQHEHHTALGWFGRLRARGDAGHAGALHLKYDAALPLVETVRLLALAH
                     GVAATGTPARIDALAAAGVLDADERDWLRASFHLIAGLVIRQQVADFRAGREVGYWLD
                     PASLRARERDRLVDDLRAIEALRGRVRSDFTGAIF"
     gene            15655..17016
                     /locus_tag="VGB88_12490"
     CDS             15655..17016
                     /locus_tag="VGB88_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9648314.1"
                     /translation="MASAQGGRGASLALALAALGGIALAAAGTAAAWAWPAHSAMALA
                     AALAALALAAGTLGALALRLVRLYDGLDRVRGDLAAVAAHRVPAGRPGPAHGLDADAA
                     RLAALVADLGGAIAEAEARRDSRLEAVLASLAEAVVVVTEAGLVSLVNAPAKALLDGA
                     RVAVGTSVYAALERDGAVAAAARARAAGMPVEAGLATAAGATLTARVADLGGHAGTVY
                     CFPGANGSHGHGLEHDLSLHDRAPAAPPPAPDTPLAALPVLVIDLETTGLEVRTDRVV
                     SIGALRAMGARVFRGELVDLVVDPGRPIPRLATAIHGITDAMVAGAPRFAEVWPEVAG
                     LFRDRIAVGHNVGFDLAVLRAECARAGLDWAEPAALDLARLAAALEPREPDMTLDGLA
                     LRWGVPVIGRHTALGDALLSAELWRRALPRLAERGVTSLAEAHAFAARARALVARQRQ
                     MGW"
     gene            complement(17019..18395)
                     /locus_tag="VGB88_12495"
     CDS             complement(17019..18395)
                     /locus_tag="VGB88_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9648315.1"
                     /translation="MDTDSGCLSIVIVGAGPAGFYAADALLRKLSACRIDIVDRLPTP
                     FGLVRSGVAPDHQSTKNVIRLYDRILQHDAVRLLGNVELGADVAYGELKRLYDVVVLA
                     FGAQVPRQLGVLGESLEGVVDSVSFVNWYNAVPDALDLSADVSGARAAVVVGNGNVAL
                     DIARLLAKTPAELAEGDIDPEAARAIAGAPLTDIYVVGRRGPLQASFTSPELAELGEL
                     AEAVPVVDAADLPESAASVPAAERPKKEKNLRILREFADADGAADPRPVKVRFVFAAS
                     PDEILGEDHVRGVRFERNRIEGARALPTGETFEVAAQLCVTAIGYRASGGGDGPPIDE
                     RRGIVANADGRVEPGVYAVGWARRGPTGVIGTNRNDAREVVDAIVAEVEPGAKPGPAG
                     LDALLAGRGVRVVDYQGWLRIDAAEVAAAGGDPKRPRVKLARVEDLLRAAEADAGAKR
                     STAGKAAG"
     gene            complement(18483..18962)
                     /gene="paaI"
                     /locus_tag="VGB88_12500"
     CDS             complement(18483..18962)
                     /gene="paaI"
                     /locus_tag="VGB88_12500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX9648316.1"
                     /translation="MNERHDPDPGAEAIAQSVYRAMEANDRVFIHYAMRALEVGPGRC
                     VLEMDVREDMLNGHDVCHGGVLFALADTAFAVACNSHNRVALAAGAEVSFVAPARVGE
                     RLRAEASERVVRKRTGIYDVTVTGQDGRLVALFRGRAHRLDVKVVDGLPADSEGDAR"
     gene            complement(18959..20494)
                     /locus_tag="VGB88_12505"
     CDS             complement(18959..20494)
                     /locus_tag="VGB88_12505"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9648317.1"
                     /translation="MTAIADGAPVAVLGAGTMGGGIAQVAAVAGHPVTLFDVTEGAVE
                     RVLEEIARRLARAVERERMTAAARDATLARIRPSVSLADAKSAALVIEAVVEELGVKH
                     GLFRELELIVAPEAVFASNTSSLSITALAAGLRFPERVVGMHFFNPAPVMALVEVVSG
                     LGTGRAVADAVYALAERWGKSPVHARSTPGFIVNRVARPFYGEGLRLLDEGAADVATI
                     DAVLREAGGFRMGPFELIDLIGLDVNLAVSRSVHAAFHGDPRYAPSIVQQDYVDAARL
                     GRKSGRGFYDYGQDAVRPRPATAPGAPAPSAVLVEGNLGPAEGLVDAIAAAGIAVTRD
                     EAGPAGPMLVLDGAALALTDGRLAAERAHEPRHPLVLFDLALDWAACTRVAVAPAPNA
                     GPAALMRACGLFQALGKQVSVIADSPALVVMRTVAMLANEAADSVLHRVCDAAAVDTA
                     MRKGVNYPDGPLALADRLGPARVLAVLDNLARLYGEDRYRASLLLRRLVAANARFHEA
                     SRQ"
     gene            complement(20491..23643)
                     /locus_tag="VGB88_12510"
     CDS             complement(20491..23643)
                     /locus_tag="VGB88_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX9648318.1"
                     /translation="MIFDLFIRRPRLAMVISLVITLAGLIAITVIPVAQYPDIAPPTV
                     RVSASYPGADAEVVEQSVAQPLEEAVNGVDGMRYMKSTSASDGSYTLTVTFAVGTDPD
                     IDTVNVQNRSALATSRLPEEVRRTGLTINKVSTDLLQVFLFYSPDGRYDQLFLSNFVT
                     LNILDEIKRVPGVGNASIFGARDYAMRVWFDPERLSNYGLATGDVIGAIRSQNVQAAA
                     GRIGAAPLSPDQRLQLTVTTKGRLATIEEFENIVVRAAAAGALVRVKDIARVELESAS
                     FDTMARYQGGPTAPVGVYLSPGANAVSVAEAVSARVESLRPRFPEGFDYAYVYNTAEF
                     VNAMIGKVLTTLVEAFALVALVVFVFLGRLRATVIPLVAVPVAIIGAAAVMLAFGYSA
                     NTISLLALVLAIGIVVDDAIIVVENVERVMEEEPELSPAAATRKAMSEIVGPIIAITL
                     VLLSVFVPVAFLPGSSGELFRQFAVAISAAMVISAVNALTLSPALCAMVLRPGRPVAV
                     MRAVTAGIARIGRGYAWTVRGLVRAAPLGVVAVAAAGFAIFWLFARTPAGFLPDEDRG
                     YIIVVMQLPPGASLNRTDAAALKAEEIIRQDPAVATVVSVLGLDFLGGGAASNTGVMF
                     ARLKPYAERETRALHALAAVGRLYRALATIPEGTFIPLNPPAISGIGQVGGFEYVLEG
                     FEGQAPADMAAAMRALVVAANQDARLAGVFSTFEATTPRVRLDIDRTKAAALGLDLSD
                     VFATLQATLGGYYVNDFNLFGRTWTVRMQAGQPYRDAIGDIAAIHVRNAAGGMTPLAA
                     IAETKLDVGPRTLTRYNNYRAVSINGGPAPGRGPGDATAAMEALSAATLPAGYGYEWT
                     GQALEQKQSAGQTPIVLGLAVLFAYLFLVALYESWNVPIAVLLSVVVAALGALAAIWA
                     FAMSFVLYTQIGLVVLIALAAKNAILINAFALERRTAGLSVAAAAAEGARMRFRPVMM
                     TSFAFIMGLVPLVVAVGPGAGSMVAVGVPVLAGMLAATLVGIFLIPMLFVAFQWLRER
                     TGWRPPQPAGDGARQGAAGR"
     gene            complement(23640..24773)
                     /locus_tag="VGB88_12515"
     CDS             complement(23640..24773)
                     /locus_tag="VGB88_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9648319.1"
                     /translation="MDRSVRAGFASLAVPPWLALLAGVACAQAPGGREAGAPGVIVEP
                     AAERDISESWSFTGRVQAIDKVELRARVQGYLAARNVEEGAEVARDQLLFVIEKAPYQ
                     AGVAQAEANLASASAAEELARVTLGRTETLVSRETVSRAALDEANAQYLQAQAAAQAA
                     EAALTRARLDLAYTDIRAPMAGRIGRAAFSVGDLVGPDAGPLATLVAQDPMYAAFPVP
                     SRVLLEVRREGRARESVSVHLELADGSLYEHAGEVRFAEVEADPTTDTVTVRATVPNP
                     DRLLVDRQLVGVTVEAKEPAARLVVSQSALLLDQQGAYVLVVDANGQVAARRVALGGQ
                     QGAQVIVESGLAAGEQVIVGGVQKVRPGMTVSPQMVSGSDPAR"
     gene            24992..25285
                     /locus_tag="VGB88_12520"
     CDS             24992..25285
                     /locus_tag="VGB88_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648320.1"
                     /translation="MLKVVTIGRIKYLSARVVAQIGATIAMAACAFIGYQLHALTSDS
                     RFVATFWRTYRDVTGTVASFFDMDPIVATIFYPAVIVGMLILGGLYLWRQGDG"
     gene            25503..26120
                     /locus_tag="VGB88_12525"
     CDS             25503..26120
                     /locus_tag="VGB88_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9648321.1"
                     /translation="MTDVLVGGPGAAAPTLVLAHGAGAPMDSPFMTAIAAALARRGVR
                     VARFEFPYMRARRAGAGRRPPDRQPVLLDAWREACAKLGASARLVVGGKSMGGRMASL
                     IADEVGAAGLVCLGYPFHPPGRRERTRTAHLEALATPALIVQGSRDPFGDPEDVTGYR
                     LSPAIAIHWIADGDHDLKPRKASGRTHADAIAEAADAVTRFVDGL"
     gene            26251..27090
                     /locus_tag="VGB88_12530"
     CDS             26251..27090
                     /locus_tag="VGB88_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX9648322.1"
                     /translation="MKVCYIDEAGDLGALADPPQPNDQPVLVIGGLFLDTDSIEAVTN
                     EFLGLKQLFFPGLPYRSPTHLDRILPEIKGAEVRSNATRGSAAQRHHAIGFLDRVVGL
                     LRRHEAKLVARIWIKGVGLPFEPTPVYTSSIQGICSYFDHYLGQSQAAGFCIADSRNK
                     FKNVNVSHSIFTQKYSPVSRNYRQILELPTFAHSDNHAGLQICDLVCSALLYPIACYA
                     YCTGHVVNVHVQPGAAALKQRYGQAIRDLQYRYLDADTGRYRGGLVVADAIAQRNASL
                     MFR"
     gene            complement(27187..28542)
                     /locus_tag="VGB88_12535"
     CDS             complement(27187..28542)
                     /locus_tag="VGB88_12535"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX9648323.1"
                     /translation="MSEAGGGHDDSVGARLRRFARVSTHVGGLAARLAGERYLGVPID
                     RRRHAEDLRRALGGLKGPLMKVAQLLSTIPEALPAEYAGELAQLQADAPAMGWLFVRR
                     RMASELGPDWQAKFASFERSAARAASLGQVHRARAFDGRELACKLQYPDMRAAVEADL
                     AQLKLIFAVYRRYDPTIDPGEIHAEIAARLHEELDYQREARHIRLYAAMLGREAGVHV
                     PEVVDALSTRRLLTMTWLEGRPLLAFKDAAPEVRNAIAGNLFRAWYVPFYDYGVIHGD
                     PHLGNYTVREDHAINLLDYGCVRIFQGRFIKGVIDLYRALSDGNEELAVEAYRSWGFQ
                     DLTRELIDVLNLWAAYVYAPLLEDRDRRIQEGGAGHYGHDVADKVHRELRRLGGVRPP
                     REFVFVDRAAIGLGSVFMHLRAEINWHRLFERLIGGFDADALDRRQRDACAAVGLEPP
                     E"
     gene            complement(28539..30356)
                     /locus_tag="VGB88_12540"
     CDS             complement(28539..30356)
                     /locus_tag="VGB88_12540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX9648324.1"
                     /translation="MTATTAIDRRAEIGELPSWDLGDLYAGPDAPAIEADLAGAGAEA
                     RALAERYRGRLASLAGAELAELVQGYERLTDTLGRISAYANLHHARDMSDPEAGRFLQ
                     TMQERITDIGTLVLFVTLELNRIDDEVLERQLAAPELAHYRPWIRDLRAFRDHQLSDD
                     LEQLLHEKYVAGRAAWVRLFDESMARLRFPFQGEQLTSAQALDLLSAKDGAVRREIAG
                     VLSEVLGRNIHTFALITNTLAKDKEIEDRWRHYPRPISQRNLENLVEDEVVEALIGAV
                     RAAFPRLSHRYYALKARWFGVEALDHWDRNAPLPEDEDRIVPWAEAERIVLDAYAGFA
                     PELADIGRRFFDRRWIDTPVLPGKAPGAFSHPTVPSVHPFILMNYQGRARDVMTLAHE
                     LGHGVHQVLAADQGALMAQTPLTLAETASVFGEQLAFRAMLEREQSPVRRRVMLAGKV
                     EDMLNTVVRQVAFCDFEQRVHDRRRGGELTADQIGDIWLAVQSESLGPALRLGDDYRC
                     YWSYIPHFIHSPFYVYAYAFGDCLVNALYALYQEHGQGFRERYLDMLRAGGSLRHREL
                     LAPFGLDAGDPAFWDKGLGVIESFIDQLEGELKGEAGAA"
BASE COUNT         4429 a        10762 c        10723 g         4445 t
ORIGIN      
        1 gtcgttgagc gcattgtacg ggcggccgtt gtacaggccg gacaggatgt aggtcttgcc
       61 ggtcgccatg tcggtcttga tcagcatgcg cccgccaaag tcggcgccga gggcggcgag
      121 catgttgccg tcgcggtcga acttgaggcc gttcgacatg cccgacggcg agcggaagat
      181 ggtcgactcg ccgctcttcg ggtcatagcg ccagatgttg ccggcctgga cgtacatgcc
      241 cgacgggtcc ttgcagaact tgctgaaggt aatgtcgctg aagtacatgt agccgtcatg
      301 gccggcggcg acgccctcgg tcaggatgca gccgccgtcg aacacgcgca tcagcttcga
      361 gcccgcgggc acgaacgcgg catcgccggt cggcgccggg aagccggcgt ccatgccctc
      421 gtaatactgt gccgaagccg agccagcggc ggcggccacc agcgccgccg cggccgcaaa
      481 accgatcaga tgcttgaagc tcattgtcat cctccctgat gagtcgagtc cgtgccggcc
      541 tgcgcggccg gcccggcaat gggggcgatc cgtgaccgac cgctccccgg atcgggcacg
      601 tcgccggcag cacgcaggtg gagcgcgtgc cgggtcggaa cccgagatcg ccgcaacgat
      661 accccatcgg gcgtgccgag atgcaagagt ttctgccccg gcgttcagcg gatcgccgcc
      721 ggcagccaca gcgcgatgtc ggggaccagc agcaccatgg cgaggccgac cgcctgcagg
      781 ccgatgaacg gccacatggc attgtagatg ttggcgagct cgattccggg cggcgccacc
      841 cccttgaggt agaacgccgc cggcccgaac ggcggcgtca ggtagctgat ctgcatgttc
      901 atgcagaaca ggatgccgaa ccacaccggg tcgtagccca gcgcgacgac cgccggcacg
      961 aagatcggca tggtcaacag gcagatgccg atccagtcca tgaacatgcc cagcaccacc
     1021 agcaccgcca tcatcaccac gacgatgccg agcggcccga acggcaggcc ggtgatcagg
     1081 cctttgacga aggcgttgcc gcccatcagg ttgtagaccc cgatcagcgc ggtggcgccg
     1141 aaggtgatcc acagcagcat gccgcaggtg ttcatggtct ggaacagcgc ctcgcgcagc
     1201 atcggccagc tcagctcgcg gcgcaccgcc gccgagacga tcgcgccgac cgcgcccatg
     1261 ccggcggcct cgctgaccga ggcgatgccg aggtagatcg agccgagcac ggccccggca
     1321 acgcccagcg gcagcaccag gccgccgatc agccttagct tctcgcgccg cggcaggttg
     1381 cgctcctcga cgggcgccgg cggccccagc tccgggttga tctggcagcg cagcgcgatg
     1441 taggcgacgt agatggtcgc cagcagcagg cccgggatga tggtggcgac gaacagctcg
     1501 ccgatgtcga cgccggcggt cagcccgtag atgatcagca cgatcgaggg cgggatcatg
     1561 gtccccagcg agccgcccgc gcagatggtg ccgatggcga gcttgcggtc gtagttcagc
     1621 cgcagcatct gcggcagcgc gacgaggccc agcaggatga tctcgccgcc gatgatgccg
     1681 gtcatcgccg ccatcagcac cgccaccacc atggtctgga tggcgagccc gccgcgcagg
     1741 ccgccggccc agatgtgcat ggcgcggtac aggtcgcgcg ccacgcccga gcgctcgagc
     1801 accgacgcca tcaggatgaa catcggcacc gcgaccagca cgaactcgcg cacgaagctg
     1861 aagatgcggc tctcgatcag gaacaggccg ggcatgccga actcgccgag cgcgaacgcc
     1921 atcgccacga agccggtgac gaaggcgagc ggcaggccca tcaccagcag cacggcgatg
     1981 gcggacacca tcaccacggt cgcgagctcg atgctcacgg cggcggctcg cgatcacggc
     2041 tgcgccggct gaggtcgcgg aaaagctggc cgatgccgag cgcgaacatc gccgcacagg
     2101 cgaacaccat cgcgctcttg acgatcggcg gcagcggcag gcgcgaggct gtgcccgcgg
     2161 tctccatgat gctgatcgac ttccaggcgt cgagcgagga ctgccagccg agcagcccga
     2221 cgaaagcgag catgatcagc tcgttgacca cgtcgagcgc gcgccgcgcc gcggccggca
     2281 tgcggtcgta aaggagcgtg atgcggatat gcgcgcgccg ctgcagggta tagacgccgc
     2341 tgaccacgaa gccgaccgcg gacagcgtcg tggtggtgtc gtgcgcccac acggtcgggg
     2401 cgccgaaggc gtagcgcgag accacctcga aggcgatgat cagcatgctg gcgaggaaca
     2461 ggtagctgaa cagctcgccg gtccgtcgcg cgaagcggtc gaccgccggc tcggctcgtg
     2521 gacgctcgac cacggccggc ggttagtcgg tgagcaggcc gatggtgcgc aggaacttga
     2581 tctgcgcctg gtaggcgcgc tcggcgcccg ggctgcgctt ggcccagtcg cgccacacct
     2641 cgatcgcgat gttgcgcagc ttctggcgct cgctcgccgg ccagtcgacg atctcgaccc
     2701 cggcgccgcg gtcggcggcg acgtcggcca ggtcctggag ctcgagccgg cgggtcatgt
     2761 cgcgcgcgaa gtcgcgcacc gcgacctcga ggatcgcctg aacgtcgggc gcgagcgcgt
     2821 cccagcgcgc ctggttgacc gagacctcga tcaccggcat cgagtggatg cccgggtaga
     2881 tcgggtagcg cgcgatcctg tggaagccga gctggtggtt catcgagaac gtcgcccagt
     2941 cggcggcgtc gaccacgccg cgctcgagcg cggtgaacac ctctgacgcc ggcaggttga
     3001 ccggcgcggc gccgatgcgc tggaagatgt cctgggccat gccctcgggc gcgcgcagct
     3061 tgacgccgcg gaaatcgtcg accccgcgga gcgggatctt ggagacgaac gcctccttgc
     3121 cggtggtcgc gccgccgatg tagtgcacgc cgtagcccgc atgcaggtcg gcgagcagct
     3181 gatcgccgcc gcccttgtgc aggaacatca gcatctggtc gggatggtcg taggccgcga
     3241 tcaggtcgcc gagcagcgcg aacgccgcgt ccttgcccga gaaatagacg gtcgccgtga
     3301 tatggccgtc gagcagcccg gcgccgaccg cgtccagcgt ctcgctgtac tggaccaccg
     3361 cgccgaccgg cagcaggcgc accttgagcc gcccgccggt cagcgtctcg atgccgtcgg
     3421 tgaagtcctg catgatctgg aacgcgatct cgccggtcga ggtcgcggcc tggaaggtcc
     3481 attcggtgtc ggcggcgcgc gcgggaatgg tcacggccga ggccgagatc gcgaccaggg
     3541 cgagcgcgca cccggcgcgg cggatggcgg tgacggcgcg ggcaaggcgc ggcatggcag
     3601 gcatcctcag tcgcttgcgc caagcagccc gaggcgcttg aggaaggcga tgtgcgagtc
     3661 gtagatgcgc ttggccatcg ggctgcgcgc ggcgtactcg gcccacacct cgaccgcgac
     3721 ctcgcggaac ttgcgccgct cgtccgcgct ccagctgacc agctcgatgc ccttctccgc
     3781 cgcatgcgcc gcgaccgccg cgtcctggag cgcgatctcc tcgaccatgt cgcgcgagaa
     3841 gtcgcgcacc gccatctcga ccagcgcctt gatgtcggcg ggcagctcgt tccagcggtc
     3901 cgtgttgacc gcgacgtcgg ccatcggcgc cgagtggaac ccgggataga gcgggtaggg
     3961 ggcgatcgcg tggtagccga ggtcgtcgtt catgccgagg gtgccccagt cggcggcctc
     4021 gacgacgccg cgctcgagcg cggtgtagac ctcggcgccg ggaatgttca ccggcgccgc
     4081 gccgatgcgc ttgaagatct cctgggccat gccctcgggc gcgcggatct tgacgccctt
     4141 gaaatcggcc aggctgcgga tcggcttctt cgacgggatc gactcgtgtc cccacgagac
     4201 cgcgccgacg taatacgatc cgtactgggc gaacagctcg cgcccgagct cgatgccgcc
     4261 gccgtagtcg agccacatgt gcagctggcg gacgttgtcg tagccgccgt tgaggtcggc
     4321 gagcagggag aaggcggggt ccttgccggc gaagtagccg gtgccgccgt gcatcgagtc
     4381 gagcaggccg gcggcgaccg catcgagcgt ctcgttgtag gcgaccaccg tgcccaccgg
     4441 cagcggctcg atcaccagcc ggccgccggt gatctcgccg acgcgcgcgc agaacgcctc
     4501 gaacaccttc tggttgatgc tgcccgcctg ccacagcgac tgcatcttcc acgagaactg
     4561 cggctcggcg tgaggtgcgc cgggaagcgc gagggcggcg gcgagcgcca ccgcgatcca
     4621 tcgcccgacg cgtcggctca ttcgcttcct ccctgtcggc ggcgcctggc cgccttcggc
     4681 cctgtcttgg ttccgggccg ttcccctctg catgactcta ccttgaccgg cgcgggcaaa
     4741 ccagcgccgg ccgcggtcac gagaacggcg cgcgctcgct gccctggtac agcagcgcgc
     4801 cgtgcagctc ggcggcgcgg gtgtgcgcgg cgaccgcgat cgggtcgaag tccgaggccg
     4861 gcttgggctc gcgctcgtaa tagccgaagt cgtcctcgcc gcgcaccagc accgccgagt
     4921 cgtgcggacc gttgagctgg cggacgtgcg gcgcgatgta gcggacggca aagccgatac
     4981 gccgatcgtc cgacgggttc gggttcgagc cgtggatcag gcgcacatgg tgcagcgaca
     5041 tctcgccggg acgcaggatc acgtcgaccg ctcggccctc gtcaaccgga taatcgacca
     5101 cctggccgcg ggtcagcatg ttgtccttgg cgttggtgtc gacatggggc agctggccaa
     5161 gcgtgtgggt gcccgggatc actcgcatgc agccgttctc gggcacgctc gggctcagcg
     5221 ccacccaggc ggtggccacg tcgggcacgc tcaaccccca ataggtcgag tcctggtgcc
     5281 acgagacgta gcggggatcg cgcgcgtcct tggtgaagaa cgacgatccc cagcacagga
     5341 tgttgggccc gatcacgtcc tcgaccgcat ccagcaccgt ctcgtcgcgc accaggtcgg
     5401 cgaggaaggt gtacagcaaa tgcggcttga acctgagcgt gccctcgaac ttgccgccct
     5461 tggcggcctc cgcggcctcg agcctcgccc gcagcttccc ggccgcggcc tccggcatga
     5521 tcggaaccgg gaatacgtag ccgtcgcgcc gataggcctc gatttgcgcg tcggtcagca
     5581 gtctcgacat cgtccgcctc gtcaagctgg gtcgcccgcc cgcccttaaa ccacagcagg
     5641 gcgtcggcgc caagggccgc agggggcgct aagatggccc ggccacggtg ccggcaggct
     5701 cgtgtcgtca aagggcttgc ccgcggtaca aggattcctt actttcccct tgaaaggata
     5761 tcctcatgct ggcgaagctc acctcgaaga accagttgac cctgcccaag gcggtgctcg
     5821 aggcggtcgc caaggcggac tacttcgacg tcgtcgctga ggacgggcgc atcgtgctca
     5881 cgcccgtgcg cgtggcccgc gccgaggccg tccgcgccaa gctgcgcgag ctcggcgtcg
     5941 acccgaaaga tgtcgcggcg gccgtcgggt gggcgcgcga cgccaagtca cggtgagggt
     6001 cgttctcgac accaacgtcc tggtctcggc gctgttgttc agccgcggtc aagtgtcgtg
     6061 gctgcggccg gcttggcaag atggcgggct gcgccctctc gtcagcaagg cgacggcgac
     6121 ggagttgctg cgggtgctcc agtacccaaa gttccgcctc accacagctg agcgggacga
     6181 tctgctggcg gacgtcctgc cgttctgtga aacggtcacg cttgacgatc cgcctgcaaa
     6241 cgtcccctcc tgtcgcgatc ctcacgacac gaaattcctt gagctcgcac tcgccggcgg
     6301 ggccgacgcg ctggtgaccg gcgacgcgga cctgttggcg ctggcgcccg cgttcgccgt
     6361 accgatcgtg acgcctgcgc aactgaaaga gcggctccgc gctgcccggc gccgggacag
     6421 ccccgggtgc tcggacggga gctagtactc ggttcgtgaa aagccggcac cgaggaacgg
     6481 cctcgcgctt cgagatggcc ctgcgggcct cctcagcgtg aggcctttat tttgcaatga
     6541 aaatcctcgt cctgaggagc gagcgaagcg agcgtctcga aggacgtgga ctgtatcacg
     6601 catttgcggt tcctggtacc ggccttgctc cgcaaacggg tggctgggca cgatcgcgaa
     6661 agcgaaatcc cttgcgacgt aaacaggtgg tcgcacgcgg ccgcggccaa tgtcatgttg
     6721 aacggacccg cgtcgacgtt gtgttgcgag gcgtcatgaa aatcaccgac ctcacgatca
     6781 ccctgttcgc ctggaccggc atcccgccca cctcctatgc ccgccatacc gggcggttcg
     6841 cgggcgagag ccggctcggc ctggtggccg tggcgaccga ccagggagtc accgggcatg
     6901 ccgtcatcgg cgccgcggcg cacggcgccg accaggacgc aggcacggtg gtgaagtacc
     6961 tgaagccgct gctcatcggc gaggacccgc tcgaccgcgc gcggctctac gaccgcatcg
     7021 gcgcgcgccg gcgctacgtg ccgccgcgct gcctcggcgc ggtcgacgtg gccctgtggg
     7081 acattgccgg caaggtcgcc gcgctccccg tccaccgcct gctcggcagc ttccgccatg
     7141 ccgtgccggc ctacgcctcc tcggcggtgc tcgaggcgcc cgcggcttac gccgaggagg
     7201 cggtgcgctt caaggaggcc ggctggcgcg cctacaagat ccatccgttc gggcgctggc
     7261 gcgaggacat cgagatctgc cgggcggtgc gccgcgcggt cggcgacgac tatacgttga
     7321 tgctcgattc gacctggaac tatcgctacg agcacgcgct taaggtcggg cgggcgatcg
     7381 aggagctcgg cttctattgg tacgaggacc cgctcgccga cgacgacatc tacaactgcg
     7441 tcaagctcaa ggagaagctc gacatcccgt tgatggcgac cgagtacgcg ccgggcggat
     7501 tcacggctta cgcgccgtgg ttgctgttgc gcgcgaccga ctacctgcgc ggcgacgtcg
     7561 cggtcaaggg cgggattacc gcgatggtca agagcgcgca cctggccgag gcgttcgcgc
     7621 tcaactacga ggtccatcac ggcggcaact cgcacaacaa ctgggccaac ctgcacgtca
     7681 tcgcggcgat ccccaacacc gagttcttcg aggtgctgct gccggcggcg gcgcagaagt
     7741 acggcgtcgt cgacgacctc gaggtcgacc gcgacggcct ggtccacgtc cccgaccgcc
     7801 ccggcatcgg cgccgagatc gacttcgagc tgatcgagcg gaccaagctc ggcgtgctcg
     7861 cctgaccggc ctcgccggtg cgccggcggg cgcgcgcgca ggtccttgct ttgcgccgca
     7921 aaatagcggc tccggccggg atatggtcgt tgaccggcga tcaaacgcgt ggctagagtg
     7981 cgccgtgccg cgctcccaga gggcgcggtt gtggtgtccg gggccggcgt cagcgtgctc
     8041 gcgcccaccc ggcgccaagg cgcggggtcg cgccccgggc cgcgaggtgg cgggcgagga
     8101 ggggggtctt ccatggaagc caaggtcgtc tggctgttcg tgttcgtggc cgtctactgg
     8161 gcctactgca tcttctgggg cgtcaagggg gcgctgagcg cgcgcacggc gagcgactac
     8221 ttcatcgccg ggcgcggcat ctcggtgtgg gtgttcgtgc tggcggcgac cgcgacctcg
     8281 ttctccgggt ggacgttcat gggccatccg ggcctggtct accgcgacgg cttcccgtac
     8341 gcctatgcct cgttctacgc catcaccatc ccgttcaccg gcgtgctgtt cctcaagcgg
     8401 cagtggatga tcggcaagcg cttcggcttc gtcacccccg gcgaaatgct gtccgactac
     8461 ttccgctccg acgtcatccg catcctggtg gtgatcgtcg cgctgagctt ctcgatcccc
     8521 tatctcggcg tgcagctgcg cgcttcgggc ttcctgttca acgtgctcac cgacggcatg
     8581 ctcagcgtcg acgtcggcat gtggctgctg tcggcggtgg tgatcatcta cgtcgcctcc
     8641 ggcggcctgc gcgcggtggc ctacgtggat accgtgcagt gcatcctgct gtcggcgggc
     8701 atcgtcatca tcggcctgat cacgctcagc tatgtcggcg gcctcggcgt cctgatcgac
     8761 ggcatcggtg cgcttaccca gatcgacgcc aagaccaccc ccgacggcta cagccactat
     8821 gtggcgatcc ccggggtgat ccagttcgtc gacaacagcg ccaaggccca gggcggggtc
     8881 tggaccggca tcatggtgct gacctacatg ttcgcgctga tgggcatcca gtcggcgccg
     8941 gcgttctcga tgtgggcgtt ctcgaaccgc agcccgcagc cgttcgcgcc gcaacaggtg
     9001 tgggcgtcgt cgttcggcat cggcctgatc ctgttcgtgt tcaccgccat ccagggcctc
     9061 ggcagccacc agctcggcac cgatctcgcg ttcatgcggc agtttccgga gctgacctct
     9121 gagtcggcgt ccgttctcca gaccggagtg atcgagtcgc aataccgcgg cgcgctcgac
     9181 ccgctcgacc ggagtgagac gcgcgacgtg gtggcggcgg ggatcgagca gttcaaggcc
     9241 accggcacgg taccgacatt tgccgacatc gcggcgggcg gcggccccgc cgccgagttc
     9301 gcccggcgcg tggtgagcgc gagtgcaggc accaacttca accctggggc ctcgcccgtg
     9361 ctgctcgacc tggtcgccga tctcaagcag ccggacatgc tggtgcccca gctgatccgc
     9421 ctgatgcagg acgtggcgcc gtggatggtg gggctgttgt cggtgtgcgc cctggcggcg
     9481 atgcagtcga ccggcgccgc ctacatgtcg accgcaggcg gcatgctcac ccgcgacctg
     9541 ttgaagcact acctgatgcc gagcgcgagc cacaagttgc agaagttctg gggccgcgtc
     9601 ggcgtggtgg tgatcgtgat ggcggcgctg atcgtggcca gcgcctcgtc ggacgcgctg
     9661 gtcctgctcg gcggcctcgc ggtcgcctac ggcttccaga tgtggccggc gctgatcgcg
     9721 gtgtgctggt ggccgtggct gacgcgcgag ggcgtcaccc tcggcctgat cgccgggctg
     9781 atcggggtca cgctcaccga ctcgctcggt ccggactggt tcggcatcga ggcgtggggc
     9841 cgctggccgc tgaccatcca ctccgccggc tggggcatgc tgttcaacat cggcgtcgcc
     9901 atcctggtct cgctggtcac ccagcgcgac agcgacctcg cccatcggat gaccttccac
     9961 agcttcctgc gcgagcatgc cagcctgccc gcgagcaagc gcaacctgat cccgcgcgcg
    10021 tgggcgatca cgctcatctg gttcgtattc gcgatcggcc cgggcgcggt gatcggcaac
    10081 tacatcttcg gctcgccgac cgatccgtcg agctggctgt tcgccatccc ctcgatctgg
    10141 gcgtggcagc tcctgtggtg ggcgctcggc gtgttcatga tgtggtacct cgcctacaag
    10201 atggagctgt cgacggtgcc gtcgcgcgag gtcgaggccc tgcacgagga catcggcgac
    10261 atccagctgg cgctggacaa gccctgatcg cggcgtgacc ggcgccgcgg acagcggcta
    10321 cctgctgtgg gtggtcgcgc tcgccgcggc gttgtggtgg ccgaccagcc gcctggtctg
    10381 ggtgctcagc gtgcgccgcc tcgagcgcaa gctggggcgc aggctgggtg agggcgagct
    10441 cgccgggcag aagcgccggg cgtggctgat cgcggcggcc ctcagcctga tcttctcggc
    10501 gctgttcaac ctgcgcctga tcgaccgcgg cggttgaccg cgcacgcgca atccggcagc
    10561 actcgcatca tgaccgcgga tgccaggagt gacgcgcgct ggaagggact cgcgatcgcg
    10621 ctcgcgatcg gctgcgccgc atggttcctc tacgacacct tgatcgcggc ccgcgacccc
    10681 gcgctcgacg cccagtcggc ggccgaccgc cagttcgagg acggccgcta cgagcgcgcg
    10741 ctcgccctct accgcgagcg gcttgcggtc gagcccgcca acgcctacgc gttgcgcggg
    10801 gcggcgctgt cgctgatgca gctcggccgc gacgccgacg cgctggccgc gttcgccgac
    10861 gcgatcgccg ccgaccccgg atttgccggc gcctacgcca accgcggcat cctccacgac
    10921 cgcatgggcg actggcaggc ggcggtcgcc gactacgagc gcgcgctcgc cctcgacccc
    10981 gaggtggccg acgggccgca ctggctgacc ctgttcctgc gcaaccagcc cgagcgcccg
    11041 ccaggcgtcg ccgaccggct cgcctacctc aggcaggagc tcgccaagcc cgagggcgag
    11101 cgcgtgctgc gcatgcccga gctcgaccag gcccagcgcc cctaccagca atagcgcggc
    11161 ggccgttacg cctcggcgtg cggcctcgcc aggtcgtgga acgcgaccgt tcgcaaacgc
    11221 gccgtcctaa acctcagggc gcgtgatatc atatatgata tgaggctggt gctcgacacc
    11281 gatgtcctcg tggcggcgat gcgtagccct cgcggcgctt cggcggcgct actgcgggcg
    11341 gtacgtgagg ggagtgcgac actcttgctc agcgttccgc tcgcccttga gtacgaggct
    11401 gtgtgccgac aacgccagca tcgcttggcg gcggcactga gcgacaaaga agtgaccacg
    11461 ttcttggacg cagtgatcgc gatggcggaa ccggtcgaga tgcacttctt gtggcgccct
    11521 caacttcagg acccggccga cgagatggtg ctcgaaaccg cggtcaacgg cgtggcggag
    11581 gcgctggtga ccttcaactc gagagacttc ggcgcggccc cgtcgcggtt cgggatcgag
    11641 atcctcctgc cacgcgacgc attgacgagg attcgacgat gacgaaggca aagacctaca
    11701 gcctgcgact tccgcgctcg ctcaaagagg cggtcgcaga gttgagccga gaggaaggga
    11761 cgagcatcaa ccagttcgtc gccaccgcag tggccgagaa ggtctccgcg ctcagtacgg
    11821 ccaggtattt cgcgcaacgg cgcgcacgtg cggacctcgc cgccttcgac cgcctcatgg
    11881 gtcggtccgc gggcgagacg ccgcgcgagg gcgacgagat cgcggaacat gggcctgggc
    11941 gggtcaggaa ccgcaaggtc tcgccgggtt gccggcgcaa acgatcgtga cggtgcccga
    12001 gctcgaccac gcccagcgcg cctaccggca gtagcgcggc gaccaaccta cttcacaacc
    12061 gccagcagca accgtaggct gggtaatcgg ccttcccgca cctgcgcggt ccatgaccat
    12121 gcccaatttg atcatcagga tcggccaggc ccttcgcttg gcgtcgcggc tcgcgcggct
    12181 gactggcgag acgctgaccg aggccgttgc tgaggcgctg cgcgagcggc tggaaaggga
    12241 agagcgccgc cgccaacgca ccggcatcgc ggcccgtctg atggagattg gccgccgttg
    12301 ctctgccgtg ccgatgcgcg acgcacgatc tcgcgaagag attcttggtt acgacgagcg
    12361 cgggctcccg cagtgacggc ggtatcgccg tcgcgacggc aagattcgtg gcccattcgt
    12421 ccgtccccat cgggcggcgt caccccggag cgcccgcaag gcgaatccgg ggtccatagc
    12481 gcggcgcgca ccgccggatg gatggcggct ctttggccgg catgacggaa tagccgcgct
    12541 cacacgaggc caagcacgtc gaccagcagc acgcgcagga agaacaggag cgcgatgcag
    12601 ccgaacagca gccgcaccca gtctgacttg ccgtgctcgt cgcgccaggt cagcgcgtgc
    12661 caggcgaccg cgccggtaat cagcaggaac acgacgtcga agactcccgg catcggccca
    12721 gtctgcgttt gcccggcgcc gcgccccgcg gtcagtagca gggcggcagg atgcagccct
    12781 cgggcccgtt caccatgtgc gcggtggcga cgatgaaggc gatcgtgaac gccgcccaga
    12841 tcgcgtagcg gatgccgaga tagatcatgg tcggggtcgg cggcgggtgc acgatgccgt
    12901 agtcggcgag gtcgaggcag ttcgcgtccg cccggcgcac cacctcgcgg gtgaacgcgc
    12961 gcgcccaggg cggcaccttg tcgcggtaca gatagtcgaa gaagtagcgg tcgagcacgc
    13021 gccggtcgct gtcgccggtc ggctcgccga accacgacgc ataggccatc cggaacacct
    13081 cgaactccga gacctccagc aggcctgcac tgtcgaggac gttggtcacg tcctcggaaa
    13141 ggtccggatc cagcgaaaac atgggtcgcg ctgctttctg cgggcgcgcg ctgtggccag
    13201 cgcgcgaatc cagtatagtc aatatagccg caccggcggc gccgatgaaa ccgccgccgg
    13261 cgccggcgca cacggtccca ggggaggaga cgatggcccg atcgatggta tttgccgccc
    13321 tgctggcttg ccttgccgtg cttggcgcgc gcggcgccgg cgccgcctgc ccgagcgccg
    13381 gcgaggtgga tgcgcgcgtc gccgcctatc tcgccgccca gccggtggcg agctacggcg
    13441 ccggcctcag catcgccgac gcctattgcg gccaagacct gtacgtcgcc cgtctcgagg
    13501 ccgcgctcgg gccgcgcatc ggctacaagg tcggcttcac cggcaaggcg agccaggagc
    13561 gcttcggggt taccgagccc gcgcgcgggg tgctgttcgc gccaatgatc ctcgagtccg
    13621 gcgccgagct tccggccgac tacggggtgc ggcccttgta tgaggcggac ttgatcgtga
    13681 ccatcgccga cgcgagcatc atggatgcga ccacgccgct cgaggcggcg gcggcgctcg
    13741 gcgccgtggt gccgttcatc gagctgcccg acatcgtcgt gcccgagggc gagacgctgg
    13801 atgcggccaa catcatcgcg ctcaacgtga tgccgcgcta cggcgtggtc ggccccggca
    13861 tcccggtcga cgcggacgag gcgttcgtcg ccgccttcgc cgacatggag gcgatcgtga
    13921 tcgacgagac cggcgccgag atcgagcgca gccggggctc ggcgctgctc ggcaacccgc
    13981 tcaatgccat gctgtggctg gtcggcaacc tgaaggcgtc cggcctcgcg ctcaagcccg
    14041 gcgacatgat cagcctgggc tcgctcggca agctttatcc gaccgcgccg ggcaagacca
    14101 tcaccatgcg ctaccagggc ctgcccggcg gcaccagcga ggtcagcgtg cgcttccgct
    14161 gagcggccgc gggcggcggc ggtcggggcg tgccgccatg gcctcgcaga ccgcggtgtt
    14221 cacccgcgtg gtcggcgacc acatgcgcgc ggcgccgccg accgtgcccg ccgggacgcc
    14281 catcgccgat gccctcgcgc gcctggtcgc ggccaaggcg acggcgctga tcgtcaccga
    14341 tcagggcggg cgcatcgccg gcatcgtcac cgagcaggac gtcgcccggc gcatcgcctg
    14401 ccgcgccggg cgcaccgccc cgatcgagca ggcgatgacc aagccggtcg ccaccgtccg
    14461 cagcgacgat ccgctctacg ccgcgatcgg ccagatgcgc aaacgcgccc ttcgccacct
    14521 gccggtggtc gacgccgccg gcgcaccggt ggggatgatc gagctctacg ccgcgctgca
    14581 ggtggcggcg gcgcagatgg tcgaccagat cgaccgcctg acccacgacg cgaccgaggc
    14641 cggcctcaag gcggtcaagg gcgagcaggt cgccgtcgcc gagcagctgt tcgccgatgg
    14701 cgtcgccggc ggcgaggtgc tggcgctgat cagccagatc aactgcgacc tttaccggcg
    14761 cgtggtcgag cgctcgctgg gcgagatggc ggcttgcggc gccggcgcgc cgccggtcga
    14821 gtttgcggtg ctggtgatgg gctcgggcgg gcgcggcgaa agcgcgatcg gcgccgacca
    14881 ggacaacggc ttcgtgctcg ccgattacga cgactccgac cacgaccgga tcgacgcgtt
    14941 cttcatcgcg ctcgccgaat cgatgaccgc gatgctcgac cgcatcggcc tgccgctgtg
    15001 cccgggcggg gtgatggcga ccaacccgct gtggcgcaag tcgctcggcc aatggcggca
    15061 tcagatcggg ttctggattc ggcgccacga cgagggcctc gtgcgcctgg ccgacatctt
    15121 cttcgacttc cgctgcgtct acggccgcga ggacctggcg cgggcgttgc gcgaggccgt
    15181 caccgagcgg atgcgcaccg cgcacgcctt cctgcgcacc atgagcacgg tccagcacga
    15241 gcatcacacc gcgctcggct ggttcggccg gctgcgcgcc cgcggcgacg ccggccacgc
    15301 gggcgcgctc cacctcaaat acgacgcggc cctgccgctg gtcgagaccg tgcgcctgct
    15361 cgcgctcgcc cacggcgtag ccgcgaccgg tacgccggcg cgaatcgacg ctcttgccgc
    15421 cgccggcgtg ctcgatgccg acgagcgcga ctggctgcgc gcgtcgtttc atctgatcgc
    15481 cggcctggtc atccgccagc aggtggccga tttccgcgcc gggcgcgagg tcggctactg
    15541 gctcgacccg gcgagcctca gggcgcgcga gcgcgaccgg ctggtcgacg acctgcgcgc
    15601 gatcgaggcg ctgcgcggcc gggtccgctc cgacttcacg ggcgcgatct tctgatggcc
    15661 tcggcgcagg gcggtcgcgg cgcctcgctg gcgctggcgc tggcggcgct cggcgggatc
    15721 gcgctcgccg ccgccggcac ggccgccgcc tgggcgtggc ccgcgcatag tgcgatggcg
    15781 ctggcggcgg cgctggctgc gctcgcgctc gccgccggca cgctcggcgc gctcgcgttg
    15841 cgcctggtcc gcctctatga cgggctcgac cgggtgcgcg gcgacctcgc cgccgttgcc
    15901 gcgcaccggg tgccggcggg gcggcccggg cccgcgcacg gcctcgatgc cgacgccgcg
    15961 cgcctggcgg cgctggtcgc cgacctcggc ggggcgatcg ccgaggccga ggcgcgccgc
    16021 gattcgcgcc tcgaggcggt gctcgcgagc ctcgccgagg cggtggtggt ggtcaccgag
    16081 gcgggcctgg tcagcctggt caacgccccg gccaaggcgc tgctcgacgg cgcccgggtc
    16141 gcggtcggca ccagcgtcta tgccgcgctc gagcgcgacg gcgcggtcgc cgccgcggcc
    16201 cgggcgcgcg ccgcgggcat gccggtcgag gccgggctcg ccaccgccgc cggcgccacc
    16261 ctcaccgccc gggtcgcgga cctcggcggc cacgccggca cggtctactg ctttcccggc
    16321 gctaacggca gccacggcca cggcctcgag catgatctgt cgctccacga ccgtgccccg
    16381 gcggcgccgc cgccggcgcc cgacaccccg ctcgccgcgc tgccggtgct ggtcatcgac
    16441 ctcgagacca ccgggctcga ggtgcggacc gaccgggtgg tctcgatcgg cgcgctacgc
    16501 gcgatgggcg cgcgcgtctt tcgcggcgag ctggtcgacc tggtggtcga tcccgggcgg
    16561 ccgatcccga ggctcgcgac cgcgatccac ggcatcaccg acgccatggt cgccggcgcg
    16621 ccgcgcttcg ccgaggtgtg gccggaggtc gccggcctgt tccgcgaccg gatcgcggtc
    16681 ggccacaatg tcggcttcga cctggcggtg ctgcgcgccg agtgcgcacg cgcggggctc
    16741 gactgggccg agccggcggc gctcgacctc gcgcgcctcg ccgcggcgct cgagccgcgc
    16801 gagcccgaca tgaccctcga cggcctggcg ctgcgctggg gggtgccggt gatcgggcgg
    16861 cataccgcgc tcggcgatgc gctgctcagc gccgagctgt ggcgccgcgc gctgccgcgc
    16921 ctcgcagagc gcggggtgac cagcctcgcc gaagcacacg ccttcgcggc gcgcgcccgc
    16981 gcgctggtcg cgcgccagcg ccagatgggc tggtaggctc agcccgccgc cttgccggcg
    17041 gtgctgcgct tggcgccggc gtccgcctcg gcggcgcgca gcaggtcctc gacgcgggcg
    17101 agcttgaccc gcggccgctt tgggtcgccg ccggcggcgg cgacctcggc ggcgtcgatg
    17161 cgcagccagc cctggtagtc gaccacgcgc acgccgcgcc cggcgagcag cgcatcgagc
    17221 cccgccggcc cgggcttcgc cccgggctcg acctcggcca cgatcgcgtc caccacctcg
    17281 cgggcgtcgt tgcggttggt gccgatcacc ccggtcgggc cgcggcgcgc ccagccgacc
    17341 gcgtacaccc cgggctcgac ccggccgtcg gcattggcga cgatgccgcg ccgctcgtcg
    17401 atcggcgggc cgtccccgcc gccgctcgcg cggtagccga tggcggtcac gcacagctgc
    17461 gccgcgacct cgaaggtctc cccggtcggc agcgcgcgcg cgccctcgat ccggttgcgc
    17521 tcgaagcgca cgccgcggac atggtcctcg cccaaaatct cgtcgggcga ggcggcgaag
    17581 acgaaccgga ccttgaccgg gcgcgggtcg gcggcgccgt cggcgtcggc gaactcgcgc
    17641 aggatgcgca ggttcttctc cttcttgggc cgctcggccg cgggtaccga ggccgccgac
    17701 tcgggcaggt cggcggcatc gaccaccggc accgcctcgg ccagctcgcc gagctcggcg
    17761 agctcgggcg aggtgaagct cgcctgcagc ggcccgcgcc ggccgaccac gtagatgtcg
    17821 gtgagcggcg ccccggcgat cgcgcgcgcc gcctcggggt cgatgtcgcc ctcggccagc
    17881 tccgccggcg tcttggccag cagccgcgcg atatcgaggg cgacgttgcc gttgccgacc
    17941 accaccgccg cgcgcgcgcc cgagacgtcg gcgctgaggt cgagcgcgtc gggcacggcg
    18001 ttgtaccagt tgacgaagct gaccgagtcg accaccccct cgaggctctc gccgaggacg
    18061 ccgagctggc gcggcacctg ggcgccgaaa gcgagcacga ccacgtcgta aagccgcttg
    18121 agctcgccat aggcgacgtc ggcgccgagc tcgacattgc cgagcagtcg caccgcgtcg
    18181 tgctgcagga tgcggtcgta gagccggatc acgttcttgg tgctctggtg gtcgggggcg
    18241 acgcccgagc gcaccaggcc gaacggggtg ggcaggcggt cgacgatgtc gatgcggcag
    18301 gcggacagct tgcgcagcag cgcgtcggcg gcgtagaagc cggcgggccc ggcgccgacg
    18361 atcacgatcg acaggcagcc gctgtccgtg tccatgcgtc tccccccttc acgcacgtgc
    18421 tcgccggtgc ggcgcgatca tagaagggcg cgcgcgcatc cggcaaccgg gcgcccgccc
    18481 gctcagcgcg cgtcgccttc cgagtccgcc ggcaggccgt cgacgacctt gacgtcgagg
    18541 cggtgggcgc ggccgcggaa cagcgccacc aggcggccgt cttgcccggt cacggtcacg
    18601 tcgtagatgc cggtgcgctt gcgcaccacg cgctcgcttg cctcggcgcg caggcgctcg
    18661 ccgacgcggg cgggggcgac gaagctcacc tcggcgccgg cggcgagcgc gacccggttg
    18721 tgcgagttgc aggcgacggc gaaggcggtg tcggcaagcg cgaacagcac gccgccgtgg
    18781 cagacgtcgt ggccgttgag catgtcctcg cgcacgtcca tctcgagcac gcagcgcccc
    18841 gggccgacct cgagcgcgcg catggcgtaa tggatgaaca cccggtcgtt tgcctccatc
    18901 gcccgataca ccgattgggc gatcgcctcg gcgccggggt ccggatcgtg ccgctcgttc
    18961 attgtcggct cgcttcatgg aaacgcgcat tcgcggcgac cagccggcgc agcagcagcg
    19021 aggcgcgata gcggtcctcg ccgtacagcc gcgccaggtt gtcgagcacc gccagcaccc
    19081 gcgccggtcc gagcctgtcg gcgagcgcga gcgggccatc ggggtaattg acgcccttgc
    19141 gcatcgcggt atcgaccgca gccgcgtcgc acacccggtg cagcaccgag tccgcggcct
    19201 cgttcgcgag catggcaacg gttcgcatga ccaccagcgc cgggctgtcg gcgatcaccg
    19261 agacctgctt gccgagcgcc tggaacaggc cgcaggcgcg catcagcgcc gccgggccgg
    19321 cattcggcgc cggcgcgacg gcgacgcgcg tgcacgcggc ccagtcgagg gcgaggtcga
    19381 acagcaccag cggatggcgc ggctcgtgcg cgcgctcggc ggcgagccgg ccgtcggtga
    19441 gcgcgagcgc ggcgccgtcg agcaccagca tcggccccgc cggcccggcc tcgtcgcggg
    19501 tgacggcgat gccggccgcg gcgatcgcgt ccaccaggcc ctcggccggg ccgagatttc
    19561 cctcgaccag gaccgcggac ggcgccggcg ccccgggcgc ggttgcgggc cggggcctga
    19621 ccgcgtcctg gccgtagtcg tagaagccgc gccccgactt ccgccccagc cgggcggcgt
    19681 cgacgtagtc ctgctggacg atcgagggcg cataccgcgg atcgccgtgg aaggcggcgt
    19741 gcaccgacct ggacaccgcc aggttgacgt cgaggccgat caggtcgatc agctcgaacg
    19801 gccccatgcg gaagccgccg gcttcccgca gcacggcatc gatggtggcc acgtcggccg
    19861 cgccctcgtc cagcagccgc aagccttcgc catagaacgg gcgcgccacc cggttgacga
    19921 tgaaccccgg cgtcgagcgg gcatgcaccg ggctcttgcc ccagcgctcg gcgagcgcat
    19981 agaccgcgtc ggcgaccgcg cgcccggtgc cgaggccgct caccacctcg accagcgcca
    20041 tcaccggcgc cgggttgaag aagtgcatgc cgaccacgcg ctccggaaat cgcagcccgg
    20101 cggcgagcgc ggtgatcgac agcgacgagg tgttgctggc gaacaccgcc tcgggcgcca
    20161 cgatcagctc gagctcgcgg aacagcccgt gcttgacgcc gagctcctcg accaccgcct
    20221 cgatcaccaa tgccgcgctc ttggcgtcgg ccagcgacac cgacgggcgg atgcgggcga
    20281 gcgtcgcatc gcgcgccgcc gccgtcatgc gctcgcgctc gaccgcgcgc gcgaggcggc
    20341 gcgcgatttc ctcgagcacg cgctcgaccg cgccctcggt gacgtcgaac agggtcaccg
    20401 ggtgcccggc gaccgccgcg acctgggcga tgccgccgcc catggtgccg gcgccgagga
    20461 ccgcgaccgg cgcgccgtcc gcgatcgcgg tcaccggccc gcggcccctt gccgcgcgcc
    20521 gtcgccggcc ggctgcggcg gccgccatcc ggtccgttcg cgcagccact ggaaggccac
    20581 gaacagcatc gggatcagga agattccgac aagcgtcgcc gccagcatgc cggccagcac
    20641 cggcacgccg actgcaacca tgctgccggc gccggggccg accgcgacca ccagcggcac
    20701 caggcccatg atgaaggcga agctggtcat catcaccggc cggaagcgca tgcgcgcgcc
    20761 ctcggccgcc gccgccgcga ccgacagccc cgcggtccgg cgctcgagcg cgaacgcgtt
    20821 gatcaggatc gcgttcttcg ccgccagcgc gatcagcact acgagcccga tctgggtgta
    20881 gagcacgaag ctcatggcga acgcccagat cgcggcgagc gcgccgagcg cggccaccac
    20941 caccgacagc agcaccgcga tcggcacgtt ccagctctcg tacagcgcga ccaggaacaa
    21001 ataggcgaac agcaccgcga ggccgagcac gatcggggtc tggccggccg actgcttctg
    21061 ctcgagcgcc tggccggtcc actcgtagcc gtagccggcg ggaagggtgg ccgccgacag
    21121 cgcctccatc gccgcggtgg catcgcccgg gccgcgcccc ggcgccggtc cgccgttgat
    21181 cgagaccgca cggtagttgt tgtagcgggt gagcgtgcgc gggcccacgt cgagcttggt
    21241 ctcggcgatg gcggcgagcg gcgtcatccc gccggcggcg ttgcgcacgt ggatggcggc
    21301 gatgtcgccg atagcgtcgc ggtagggctg cccggcctgc atgcggaccg tccaggtgcg
    21361 gccgaacagg ttgaagtcgt tgacgtagta gccgccgagg gtggcttgca gggtggcgaa
    21421 cacgtcgctc aggtcaagcc ccagcgccgc ggccttggtg cggtcgatgt cgaggcgcac
    21481 ccgcggcgtc gtcgcctcga aggtcgagaa cacgccggca aggcgcgcat cctggttggc
    21541 ggcgacgacc agtgcgcgca tcgccgccgc catgtccgcg ggcgcctggc cctcgaaccc
    21601 ttcgagcacg tactcgaagc cgccgacctg gccgatgcct gagatcgccg gcgggttgag
    21661 cgggatgaag gtgccctcgg ggatggtggc gagcgcgcgg tagagccgcc cgaccgcggc
    21721 cagcgcgtgc agcgcgcgcg tctcgcgctc ggcgtagggc ttgagcctgg cgaacatcac
    21781 cccggtgttc gacgccgcgc cgccgccgag gaagtcgagg ccgagcacgc tgaccacggt
    21841 ggcgaccgcc ggatcctggc ggatgatctc ctccgccttg agcgcggcgg cgtcggtgcg
    21901 gttgagcgag gcgccgggcg gcagctgcat gaccacgatg atgtagccgc ggtcctcgtc
    21961 cggcaggaag cccgcgggcg tgcgcgcgaa cagccaaaag atcgcgaacc cggccgccgc
    22021 caccgcgacc acaccgagcg gcgccgcccg caccagcccg cgcacggtcc acgcatagcc
    22081 gcgcccgatc cgcgcgatgc ctgcggtcac cgcgcgcatc acggcgaccg gccggcccgg
    22141 cctgagcacc atcgcgcaca gtgccggcga gagcgtcagc gcgttgaccg cggagatcac
    22201 catcgccgcc gagatcgcga ccgcgaactg gcggaacagc tcgcccgacg agcccggcag
    22261 gaaggcgacc ggaacgaaca ccgacagcag caccagggtg atcgcgatga tcgggccgac
    22321 gatctcggac atcgccttgc gggtcgccgc ggcgggcgac agctcgggct cctcctccat
    22381 cacccgctcg acgttctcga ccacgatgat cgcgtcgtcg acgacgatgc cgatggcgag
    22441 caccaatgcg agcagcgaga tggtgttcgc cgagtagccg aaggcgagca tcaccgccgc
    22501 cgcgccgatg atcgcgaccg gcaccgcgac cagcgggatc acggtcgcgc gcagcctgcc
    22561 caggaacacg aacaccacca gggcgaccag cgcgaacgcc tcgaccaggg tggtcagcac
    22621 cttgccgatc atggcgttga cgaactcggc ggtgttgtag acgtaggcgt agtcgaagcc
    22681 ctcggggaag cgcggcctga gcgattcgac gcgcgccgag accgcctcgg cgaccgagac
    22741 cgcgttggcg ccgggcgaca ggtacacccc gaccggggcg gtcggcccgc cctgatagcg
    22801 ggccatggtg tcgaacgagg ccgattcgag ctcgacgcgc gcgatgtcct tgaccctgac
    22861 cagcgcgccc gcggcggcgg cgcgcacgac gatgttctcg aactcctcga tcgtggcgag
    22921 ccggcccttg gtagtgacgg tgagctggag gcgctggtcg gggctgagcg gcgcggcgcc
    22981 gatccgcccc gctgcggcct gcacgttctg ggagcggatc gcgccgatca cgtcgccggt
    23041 cgcaagcccg tagttcgaca ggcgctcggg atcgaaccac acgcgcatcg cgtagtcgcg
    23101 ggcgccgaag atcgaggcgt tgcccacccc gggcacgcgc ttgatctcgt cgaggatgtt
    23161 gagcgtgacg aagttgctga ggaacagctg atcgtagcgc ccgtcgggcg agtagaacag
    23221 gaacacctgc agcaggtcgg tcgacacctt gttgatggtg agcccggtgc gccgcacctc
    23281 ctcgggcagg cgcgaggtgg cgagcgcgga gcggttctgg acgttgaccg tatcgatgtc
    23341 ggggtcggtg ccgacagcga aggtgacggt caaggtgtag gagccgtcgc tggccgaggt
    23401 cgacttcatg tagcgcatgc cgtcgacgcc gttgaccgcc tcctcgagcg gctgggcgac
    23461 gctttgctcg accacctcgg cgtcggcccc cggatagctg gcggagaccc gcaccgtcgg
    23521 cggcgcgata tcggggtact gcgcgaccgg gatcacggtg atcgcgatca ggccggcgag
    23581 ggtgatcacc agcgagatca ccatcgccag ccgcggccgg cggatgaaca ggtcgaagat
    23641 catcgtgccg ggtcgctgcc gcttaccatc tgggggctta cggtcatgcc cgggcggacc
    23701 ttctggacgc cgccgacgat gacctgctcg cccgcggcga gcccgctttc gacgatcacc
    23761 tgcgcgccct gctgcccgcc cagggccacc cgccgcgccg ccacctggcc gttggcgtcg
    23821 accaccagca cgtaggcgcc ctgctggtcg agcagcagcg ccgactgcga aaccaccagc
    23881 ctcgccgcgg gctccttggc ctcgaccgtg accccgacca gctgccggtc gaccagcagg
    23941 cggtcgggat tgggcaccgt ggcgcggacg gtaacggtgt cggtggtcgg gtcggcctcg
    24001 acctcggcga aacggacctc gcccgcatgc tcgtagagcg agccgtcggc gagctccaga
    24061 tggacgctca cgctttcgcg cgcgcggccc tcgcggcgca cctccaggag cacccgcgac
    24121 ggcaccggga aggcggcata catcgggtcc tgcgcgacca atgtcgcgag cggccccgcg
    24181 tccggcccaa ccaggtcgcc gaccgagaac gcagcgcggc cgatgcgccc ggccatgggc
    24241 gcgcggatgt cggtgtaggc gaggtcgagc cgggcgcggg tgagcgcggc ctcggcggcc
    24301 tgggcggcgg cctgggcctg gaggtactgg gcgttcgcct cgtcgagcgc ggccctggac
    24361 acggtttcgc ggctgaccag ggtctcggtc cggcccaagg tgacccgggc gagctcctcg
    24421 gcggcggatg cgctggcgag attcgcctcg gcctgggcga cgccggcctg gtacggcgcc
    24481 ttctcgatca cgaacagaag ctggtccctg gcgacctcgg caccttcctc gacgttgcgc
    24541 gcggcgagat agccctgcac ccgggccctg agctcgacct tgtcgatcgc ctggacgcgg
    24601 ccggtgaagc tccagctttc cgagatgtcg cgctccgcgg ccggctcgac gatcacgccc
    24661 ggcgcgcccg cctcgcgccc gcccggggcc tgggcgcagg caacaccggc gagcaaggcg
    24721 agccatggcg gcaccgccag gctcgcgaaa cccgcccgca ccgatcggtc catggccgcg
    24781 ctcctccgct ccttgctggc gacccggctg gccgagggcg accgtcccgc cgcgccctca
    24841 agctcagaca gcctatacca cagcccgcga tcaacggctg acgatcggcg ggccggccgg
    24901 ttcgagatcg aaacggcggg cgtgtgactc gagtcactgg cattcggcgg cgcccgcgct
    24961 aactcgttac ccggttgcga aggagacgac gatgctcaag gtcgtgacga tcggccgcat
    25021 caagtatctc tccgctcgtg tcgtcgccca gatcggcgcc acgatcgcga tggcggcctg
    25081 cgcattcatc ggctaccagc tgcacgcgct tacctccgat agccgtttcg tggcgacgtt
    25141 ttggcgcacc taccgcgacg tcacgggtac ggtggcgtcg ttcttcgaca tggacccgat
    25201 cgtcgccacc atcttctacc cggcggtgat cgtcggaatg ctgatcctcg gcggcctgta
    25261 cctgtggcgc cagggggacg gctgagcgcg cccaccgtga cagccgggcc ggtcgcggcc
    25321 gcctcctgac cccggaggcg gccgctgcta ttctggccgg cgccgggccg catcccggcg
    25381 cggcgcgcgg ggacggacgt ggcgcatctc aaagtgttcg cgatcgccgc cggcttcggc
    25441 cttgcggcct acgcggcggt gatggcgctg gtggcgctgg cccggctcgc cggcctcggc
    25501 ggatgaccga tgtcctggtc ggtgggcctg gcgcggcggc gccgacgctg gtgctcgccc
    25561 acggcgccgg tgcgccgatg gacagcccgt tcatgaccgc gatcgccgcg gcgctcgcgc
    25621 gccggggtgt gcgcgtcgcg cgcttcgagt tcccttacat gcgcgcccgc cgcgccggcg
    25681 ccggtcgccg cccgcccgac cgccagccgg tgcttctcga cgcctggcgc gaggcctgcg
    25741 cgaagctcgg cgcgtccgcg cgcctggtcg tcggcggcaa gtcgatgggc gggcggatgg
    25801 cgagcctgat cgccgacgag gtcggcgccg ccgggctggt ctgtctcggc tacccgttcc
    25861 atccgcccgg gcgccgcgag cgcacccgca ccgcgcatct ggaagcgctc gccacgcccg
    25921 cgctgatcgt ccagggcagc cgcgacccgt tcggcgaccc cgaggatgtc accggatacc
    25981 ggctgtcgcc ggccatcgcg attcattgga tcgccgacgg cgaccacgac ctcaagccgc
    26041 gcaaggcgag cggccgcacc catgccgacg ccatcgcaga ggcggcggac gccgtcaccc
    26101 gtttcgtcga cggtttgtaa gtccccgcct gccggttgac aatgaggttg gacctaccta
    26161 ttttgttctc agctatcacg acccgctgac ccgtcatggg cagcagaccc tatggccacc
    26221 tacgggtggc catagccatg tcaggggcgg atgaaagtct gttatatcga cgaagcgggc
    26281 gacctaggtg cgctcgctga tccaccgcag ccgaatgatc agccggtcct ggtcatcggc
    26341 ggcctgttcc tcgataccga cagtatcgag gccgtcacca atgaatttct cggcctgaag
    26401 caactcttct tcccgggcct gccataccga tcgccgaccc atctcgatcg cattttgccc
    26461 gaaatcaagg gcgcggaggt ccgctcgaat gccacgcgcg gttcagccgc gcagcgtcac
    26521 cacgcaatcg gattcctcga ccgcgtcgtg ggtctacttc gtcgtcatga agcgaagctt
    26581 gtcgctcgca tctggatcaa gggcgttggc ctcccgtttg agcccacgcc ggtctacacg
    26641 tcctcgatcc aaggcatctg ctcgtatttc gaccactacc tgggacagtc gcaggcggcc
    26701 ggcttttgca ttgccgacag ccggaacaag ttcaagaacg tcaacgtgtc ccattccatc
    26761 ttcacgcaga aatacagccc cgtgtcccgg aactatcggc agattctcga gctgccgacc
    26821 ttcgcgcaca gcgacaatca cgcggggctg cagatctgtg atctggtatg ttcggcgctg
    26881 ctgtacccga ttgcctgcta cgcgtactgc accggccacg tggtcaacgt tcatgtgcaa
    26941 cccggcgctg cggcgctcaa gcagcgctat ggccaagcga tcagggacct gcagtaccgc
    27001 tatctcgatg ctgatacggg ccgataccgt ggcggcctgg tggttgcgga cgcgatagcg
    27061 caacgcaatg cgtcgctgat gtttcgctga tcgcgcgccg taaatgaccg gacaatgcca
    27121 tggtggatgc cgtggctgtc cgtgtatcgc gctgatggcg tacctcaccc gcctgtaggg
    27181 gtcgcttcac tccggcggct cgaggccgac cgcggcgcag gcgtcgcgct ggcggcggtc
    27241 gagcgcatcc gcgtcgaagc cgccgatcag gcgctcgaac aggcggtgcc agttgatctc
    27301 cgcgcgcagg tgcatgaaca ccgagccgag cccgatcgcg gcgcggtcga cgaacacgaa
    27361 ctcgcgcggc ggccgcacgc cgccgagccg gcgcagctcg cgatgcacct tgtctgcgac
    27421 gtcgtggccg taatggccgg caccgccttc ctggatgcgc cggtcgcggt cctcgagcag
    27481 cggcgcgtac acgtatgccg cccacaggtt gagcacgtcg atcagctcgc gagtgaggtc
    27541 ctggaagccc cagctccggt aggcctcgac cgcgagctcc tcgttgccgt cgctgagcgc
    27601 gcggtagagg tcgatcacgc ccttgatgaa ccggccctgg aagatgcgga cgcagccata
    27661 gtcgagcaaa ttgatggcgt ggtcctcgcg caccgtatag ttaccgagat gcgggtcgcc
    27721 gtggatcacg ccgtagtcgt agaacggcac gtaccaggcg cggaacaggt tgccggcgat
    27781 ggcgttgcgg acctcgggcg cggcgtcctt gaacgcgagc agcggccgcc cttcgagcca
    27841 ggtcatggtc aacagccgcc gcgtcgacag cgcgtccacc acctcgggca cgtgcacgcc
    27901 ggcctcgcgg ccgagcatgg cggcgtagag gcggatgtgg cgcgcctcgc gctggtagtc
    27961 gagttcctcg tgcagccggg cggcgatctc ggcgtggatc tcgcccgggt cgatggtcgg
    28021 gtcgtagcgc cggtagacgg cgaagatcag cttgagctgg gcgaggtcgg cctcgaccgc
    28081 ggcgcgcatg tcgggatatt ggagcttgca ggcaagctcg cggccgtcga atgcgcgcgc
    28141 gcgatggacc tggccgagcg aggcggcgcg cgccgcgctg cgctcgaacg aggcgaactt
    28201 ggcctgccaa tcggggccga gctcgctcgc catccgccgg cgcacgaaca accagcccat
    28261 cgccggcgcg tcggcctgga gctgggcgag ctcgccggca tactccgcgg gcagcgcctc
    28321 ggggatggtc gacaagagct gggcgacctt catcagcggg cctttcagcc cgccgagcgc
    28381 gcggcgcagg tcctcggcgt ggcggcggcg gtcgatcggc acgccgagat agcgctcgcc
    28441 ggcaagccgc gcggcgagtc ccccgacatg ggtcgagacg cgggcaaagc ggcgcagccg
    28501 cgcgccgacg ctgtcgtcgt gcccgccgcc ggcttcgctc atgccgcgcc cgcctcgccc
    28561 ttgagctcgc cttcgagctg gtcgatgaac gattcgatca cccccaggcc cttgtcccag
    28621 aacgccggat cgccggcatc gagcccgaac ggcgccagca gctcgcggtg gcgcaagctg
    28681 ccgccggcgc gcaacatgtc caaatagcgc tcgcggaagc cctggccgtg ctcctggtag
    28741 agcgcgtaca gcgcgttcac caggcagtcg ccgaaggcgt aggcgtagac gtagaacggg
    28801 ctgtggatga agtgcgggat gtacgaccag tagcagcggt aatcgtcgcc gagccgaagc
    28861 gccggcccca ggctctcgct ctgcacggcg agccagatgt cgccgatctg gtccgcggtc
    28921 agctcgccgc cgcggcgccg gtcgtggacg cgttgctcga agtcgcagaa cgcgacctgg
    28981 cggaccaccg tgttcagcat gtcctcgacc ttgccggcca gcatcacccg ccgccgtacc
    29041 gggctctgct cgcgctcgag catggcgcgg aaggcgagct gctcgccgaa cacgctcgcg
    29101 gtctcggcca aggtcagcgg tgtctgcgcc atcagcgcgc cttggtccgc cgccagcacc
    29161 tggtggacgc cgtggccgag ctcgtgcgcc agcgtcatca cgtcgcgcgc ccggccctgg
    29221 tagttcatca ggatgaacgg gtgcacggac ggcacggtcg gatgcgagaa cgcgcccggc
    29281 gccttgccgg gcagcaccgg ggtgtcgatc cagcgccggt cgaagaaccg ccggccgatg
    29341 tcggcaagct cgggcgcgaa ccccgcatag gcgtcgagca cgatccgctc ggcctcggcc
    29401 cagggcacga tccggtcctc gtcctcgggc agcggcgcgt tgcggtccca gtggtccagc
    29461 gcctcgaccc cgaaccagcg cgccttgagc gcgtaatagc gatgtgacag gcgcgggaag
    29521 gcggcgcgca ccgcgccgat cagcgcctcg accacctcgt cctcgaccag gttctcgaga
    29581 ttgcgctgcg agatcgggcg cgggtagtgc cgccagcgat cctcgatctc cttgtccttg
    29641 gcgagcgtgt tggtgatcag cgcgaaggtg tggatgttgc ggcccagcac ctcggacagg
    29701 acgccggcga tctcgcgccg taccgcgccg tccttcgccg acaacaggtc gagcgcctgg
    29761 gcgctggtga gctgctcgcc ctggaacggg aaccgcagcc gcgccatgga ctcgtcgaac
    29821 agccgcaccc aggcggcgcg accggccacg tacttctcgt gcagcagctg ctcgagatcg
    29881 tccgacagct ggtggtcgcg gaaggcgcgc aggtccctga tccacggccg gtaatgggcc
    29941 agctccggcg ccgcgagctg gcgttcgagc acctcgtcgt cgatgcggtt gagctcgagg
    30001 gtgacgaaca gcaccagggt gccgatgtcg gtgatgcgct cctgcatggt ctgcaggaag
    30061 cggccggcct cgggatcgct catgtcgcgg gcgtggtgca gattggcgta ggcggagatc
    30121 cggcccaggg tgtcggtgag ccgctcatag ccctgcacca gctcggcgag ctcggcgccg
    30181 gcaagggagg cgagccggcc gcggtagcgc tcggcgagcg cgcgcgcctc ggccccggcg
    30241 ccggcgaggt cggcctcgat cgcgggcgcg tcgggcccgg catagaggtc gcccaggtcc
    30301 cagctcggca gctcgccgat ctcggcccgg cggtcgatag cggtggtcgc ggtcatgca
//
LOCUS       DASWHU010000333         6715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_202889, whole genome shotgun sequence.
ACCESSION   DASWHU010000333 DASWHU010000000
VERSION     DASWHU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202889"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VGB88_12545"
     CDS             <1..150
                     /locus_tag="VGB88_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9648325.1"
                     /translation="IARQAGVAESGYRVVANTGPDSGQEVMHLHFHVFAGRRLGGMRR
                     QAGSE"
     gene            complement(642..1001)
                     /locus_tag="VGB88_12550"
     CDS             complement(642..1001)
                     /locus_tag="VGB88_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648326.1"
                     /translation="MALKTIRLELARTRDFPDGNPACGYEFHAPLTADGHIDAEQWRA
                     HKTDSTVRRFWQGEDDEHGHLIHTRHRTWAFSYAPGEEDDTPFFRFEAHSFVPGEYVA
                     ITEKGGEAYPFKVVSVE"
     gene            complement(1013..1285)
                     /locus_tag="VGB88_12555"
     CDS             complement(1013..1285)
                     /locus_tag="VGB88_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648327.1"
                     /translation="MLSLQDCIDLCELTEAEIEAIAEHEHIPTVVAVELAEYLVESEG
                     GERRIRAMIRDDIQAALARSDRAHAQALVRVLRNFIATHPERETVD"
     gene            complement(1297..2157)
                     /locus_tag="VGB88_12560"
     CDS             complement(1297..2157)
                     /locus_tag="VGB88_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9648328.1"
                     /translation="MTWASILALLHGDDGDRGAVAAALAIAKAFDAGVDGLIVRPDPR
                     DAVRLAVDGMSPAMIESVIDAARSESQRRVERARATFEAACREAGVALTDAGPRGLAA
                     RWHEEVAIAADRVAYHGRLSDLVVVGRSGDRASDESDIAIETVLFETGSPLLIAAGEG
                     EGLVGGGAMVAWNGRREAARAVTAALPLLARAGSVAVVAANALDAALPGPEALCGYLA
                     RHGISAEARTVDAGAGSVPKFLLEHAAESGAGLLVMGAYGHSRLRELVLGGVTRQVLV
                     DAALPVFMVH"
     gene            complement(2237..3769)
                     /locus_tag="VGB88_12565"
     CDS             complement(2237..3769)
                     /locus_tag="VGB88_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9648329.1"
                     /translation="MIVEDQSEVVALLCDPASHGGAAVEVIETHAAVVVLAGATAYKL
                     KRAVRFPFLDFSTAAKRRVACEAEVRLNRRTAPELYRGVIAVTRGAGGALAIGGDGDA
                     VDWLVVMRRFDQDRLFDRLAARGELTAALMQELADAIADFHDGAEPRPDKGGRAGIAQ
                     VIDGNMETLRGPGAGTFKAAELDRLERLWREAVESGAGLLEARRAGGLVRWCHGDLHL
                     RNICLIDGRPTLFDGIEFNPDIACIDVLYDLAFLLMDLQHRRLGAFANLVLNRYLGRT
                     EDVAGCALLGLFQSLRAGVRAMVAAIEAGEGQAEMAGEARAYFRLALAFFDSPPARLI
                     AIGGLSGTGKSTLAAAIAPEIGAAPGALVLRSDVVRKRLFGVPPEQRLGPEAYGGDID
                     RRVYERLRTLARQGLAAGRAVIVDAVYARPAERAALAQLAESARVPFTGLWLEAAPEV
                     LRRRVGGRGADASDATEAIVTRQLAYDLGDIAWARLAADADTSALRKAALGVIARPAA
                     GD"
     gene            complement(3809..4756)
                     /locus_tag="VGB88_12570"
     CDS             complement(3809..4756)
                     /locus_tag="VGB88_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9648330.1"
                     /translation="MSAPASAPIGREDARLLRLATHASVAVAATLIVLKFGAWVLTDS
                     VAILSSLVDSVLDSFASLVNLFAVRQALTPADPEHRFGHGKAESIAGLGQAAFIAGSA
                     LFVLVEAVARLVTPHPLGYGPVGLAVMVISIVLTFALVRFQMHVVRRTGSTAISADSL
                     HYRSDLLVNGGIIASLGVSVYVGVPWVDPLVAIAIAGYIVYSAWAIAREALDALMDRE
                     FPDHDRQRILDIVAAHVQVKGIHDLRTRRSGLQPFVQLHLELDGSITLNDAHIISDQV
                     EAEIMAAFPGAEVIIHQDPHGIPETMPAYAREDAPSGPS"
     gene            complement(4753..5352)
                     /gene="pdxH"
                     /locus_tag="VGB88_12575"
     CDS             complement(4753..5352)
                     /gene="pdxH"
                     /locus_tag="VGB88_12575"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9648331.1"
                     /translation="MLIRARDDPVALFNEWLSEAVAKEPNDPNAVCLATVGAGGVPSA
                     RMVLLKGADAAGFVFFTNLGSRKGAEIKANPRAALCFHWKSLRRQVRVEGPASRVTDD
                     AADAYFASRTRQSRIGAWASKQSQPLASRYELERRVAKYAARFGLGEVPRPPFWSGFR
                     IVPLRIEFWRSGNFRLHDRLVYERDDAGDAGWRTARLFP"
     gene            5494..5970
                     /locus_tag="VGB88_12580"
     CDS             5494..5970
                     /locus_tag="VGB88_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX9648332.1"
                     /translation="MNSFKAVVAAGMILSLGACLEGQGDKQVAGTLVGAGLGGLAGSQ
                     IGSGTGQLAAVGAGVLLGGLLGSEVGRSLDKADQAYAAQTYQSTLESVPTGQTQSWAN
                     PDSGNQGTYTPTRTYQTESGQYCREFQQTITVGGEVQSGYGTACRQGDGTWKIVSN"
     gene            6201..>6715
                     /locus_tag="VGB88_12585"
     CDS             6201..>6715
                     /locus_tag="VGB88_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9648333.1"
                     /translation="MARDASQDQIKKAYRKLAKQLHPDTHPGDTKVEDRFKQVSAAYA
                     ILSDPEMRQRYDRGEVDASGQERPQWRFRQPHGADAGAGGFAGGFNVEDILGDLFGGR
                     FGAGRGAMRARGRGVSYTVRIGFEESARGGKRRVTLGGGRSLDVAIPEGIEDGQSIRL
                     KGQGEPGIGGGP"
BASE COUNT         1010 a         2436 c         2386 g          883 t
ORIGIN      
        1 atcgcgcgcc aggccggtgt cgccgagtcc ggctaccgcg tggtcgccaa caccggcccc
       61 gactcgggcc aggaggtgat gcacctccac ttccacgtct tcgccggccg ccgcctcggc
      121 ggcatgcgcc gccaggccgg cagcgagtag ccggcgcgcc gcgcgatgaa gaagaaagat
      181 taaaccacag aggacacaga gacgggtatt gcacttctgt cacgcgcggc gcgacggaac
      241 atactttctt cctctgtgac ctctgtggtt aatcgttagg ccgctagcga ataggcggcg
      301 gcgttcgccg cccggacacg cggcggggtc ataccagcga gcatatgaac gacgccgccc
      361 ctgccgtcat tccggccgga gcggagcgga gagccggaat ccagggccgc gtaatccggt
      421 cgccggaccc ccgggcgccg acggcgcttc gcgccgtcct ggaccccggg tctcggctgc
      481 gcctcgcccg gggtgacgct ttggtgtcga cgggcgccgg cgagtcaatt ggttggctcg
      541 ctggtatcag actctaagga gcgataagtt agtctggcac ggaacgcaac cgccgcgtcc
      601 tccgcgtctc cgcgtgaacc gaataatcgg cagtcagcgc gtcactcgac cgacaccacc
      661 ttgaacgggt aggcctcgcc gcccttttcg gtgatggcga cgtactcgcc cggcacgaag
      721 ctgtgggcct cgaagcggaa gaacggcgtg tcgtcctcct cgccgggggc gtaggagaac
      781 gcccaggtgc gatggcgggt gtggatcaga tggccgtgct cgtcgtcctc gccctgccag
      841 aagcggcgca cggtgctgtc cgtcttgtgc gcgcgccatt gctcggcgtc gatatggccg
      901 tcggcggtga gcggcgcgtg gaactcgtag ccgcaggcgg ggttgccgtc gggaaagtcg
      961 cgcgtgcgcg cgagctcgag ccggatcgtc ttcagcgcca tcgcggcctc gttcaatcga
     1021 cggtttcgcg ctcggggtgg gtcgctatga aattcctgag cacgcgcacc agcgcctggg
     1081 cgtgggcgcg atcgctgcgc gcgagcgcag cctggatgtc gtccctgatc atcgcccgga
     1141 tgcggcgctc gccgccctcg ctctcgacca ggtactcggc gagctcgacc gcgaccacgg
     1201 tcgggatgtg ctcgtgctcg gcgatcgcct cgatctccgc ctcggtcagc tcgcacaggt
     1261 cgatgcagtc ctgaaggctc aacatcgaac gccccgtcaa tggaccatga acaccggcag
     1321 cgcggcgtcc accagcacct ggcgggtgac cccgccgagc accagctcgc gcagccggct
     1381 gtggccgtag gcgcccatca ccaacagccc ggcgccggac tcggcggcat gctcgagcag
     1441 gaacttgggc accgacccgg cgccggcatc gacggtgcgc gcctcggccg agatgccgtg
     1501 gcgggcgaga tagccgcaca gcgcctcggg cccgggcagc gcggcgtcga gcgcgttggc
     1561 cgcgaccacc gccacgctgc cggcgcgcgc gagcagcggc agcgccgcgg tcaccgcccg
     1621 cgccgcctcg cgccgcccgt tccaggccac catcgcaccg ccgccgacca ggccttcgcc
     1681 ctcgcccgcg gcgatcaaca gcggcgagcc ggtctcgaac agcacggtct cgatggcgat
     1741 gtcggactcg tcgctggccc ggtcgccgct gcgcccgacc accaccaggt ccgacaggcg
     1801 gccgtgatag gcgacgcggt cggcggcgat cgccacctcc tcgtgccagc gcgcggcgag
     1861 cccgcgcggc ccggcgtcgg tcagcgcgac accggcctcg cggcaggcgg cctcgaaggt
     1921 cgcccgcgcg cgctcgaccc gacgctggga ttcgctgcgc gcggcgtcga tgacgctctc
     1981 gatcatcgcc ggcgacatgc cgtcgaccgc gagccggacc gcgtcgcgcg ggtcggggcg
     2041 gacgatcagg ccatcgacgc cggcgtcgaa cgccttggcg atggcgagcg ccgccgccac
     2101 cgcgccgcgg tcgccgtcgt cgccgtggag cagcgccagg atgctcgccc aggtcatcgc
     2161 ccgttccttc ccgcgtgtca gggtagcata aacgaaaccg gaccgcccct gcgcattgac
     2221 ctggatcagg atcggctcag tcccccgccg cgggccgcgc gatcacgccc agcgccgcct
     2281 tgcgcaacgc gctggtgtcg gcgtcggcgg cgagcctcgc ccaggcgatg tcgccgaggt
     2341 cgtaggcgag ctggcgggtg acgatcgcct cggtcgcatc ggaggcgtcg gcgccgcgcc
     2401 cgcccacccg gcggcgcagc acctccggcg cggcctcgag ccacaggccg gtgaacggca
     2461 ccctggcgga ctcggccagc tgcgccagcg ccgcccgctc cgccgggcgc gcatagaccg
     2521 catcgacgat caccgcgcgc ccggccgcga gcccctggcg ggcgagcgtg cgcagacgct
     2581 cgtagacgcg ccggtcgatg tccccgccat aggcctccgg ccccaggcgc tgctcgggcg
     2641 ggaccccgaa caggcgcttg cgcaccacgt cgctgcgcag caccagcgcg cccggcgcgg
     2701 cgccgatctc gggcgcgatc gcggcggcga gagtcgactt gccggtcccc gacaggccgc
     2761 cgatcgcgat caggcgcgca ggtggggagt cgaagaaggc gagcgcgagc cgaaaatacg
     2821 cgcgcgcctc gcccgccatc tccgcctgcc cctcgccggc ctcgatcgcc gcgaccatgg
     2881 cgcgcacgcc ggcgcgcagc gactggaaca gccccagcag cgcgcagccg gcgacgtcct
     2941 cggtgcggcc gagatagcgg ttgagcacca ggtttgcgaa cgcgccaagg cgccggtgct
     3001 ggaggtccat gagcaggaag gcgaggtcgt agagcacgtc gatgcaggcg atgtcggggt
     3061 tgaactcgat gccgtcgaac aaggtcgggc ggccgtcgat caggcagatg ttgcgcaggt
     3121 gtagatcgcc gtggcaccaa cgcaccaggc cgccggcgcg gcgcgcctcg agcaggccgg
     3181 cgccggactc caccgcctca cgccacagcc gctccaggcg gtcgagctcg gcggccttga
     3241 aggtgccggc gcctgggccg cgcaaggttt ccatgttgcc gtcgatgacc tgggcgatgc
     3301 cggcgcgccc gcccttgtcg ggccgcggct cggcgccgtc gtggaagtcg gcgatggcgt
     3361 cggccagctc ctgcatcagc gcggcggtca gctcgccccg cgccgccagc cggtcgaaca
     3421 gccggtcctg gtcgaagcgg cgcatgacca ccagccagtc gaccgcatcg ccgtcgccgc
     3481 cgatggcgag cgcgccgccc gcgccgcgcg tgaccgcgat cacgccccga tacagctctg
     3541 gcgcggtgcg ccggttcagg cgcacctcgg cctcgcaggc aacccggcgc ttggccgcgg
     3601 tcgagaagtc gaggaacggg aagcgcaccg cgcgcttgag cttgtaggcg gtcgcgccgg
     3661 ccagcaccac caccgcggca tgggtctcga tcacctccac cgccgccccg ccgtggctcg
     3721 cgggatcgca cagcagcgcg acgacctcgc tctggtcctc gacgatcacg gccgcagcgt
     3781 cagcgccgac cgaggcctgc gcggccgctc acgacggccc gctcggcgca tcctcgcgcg
     3841 cataggccgg catggtctcc ggaatgccgt gggggtcctg atggatgatc acctcggcgc
     3901 cggggaacgc ggccatgatc tcggcctcga cctggtccga gatgatgtgc gcatcgttga
     3961 gcgtgatcga gccgtcgagc tcgaggtgca gctgaacgaa gggctgcagg cccgagcgac
     4021 gcgtgcgcag gtcgtggatg cccttgacct ggacgtgcgc ggcgacgatg tcgaggatgc
     4081 gctggcggtc gtggtcgggg aactcgcggt ccatcagggc atccagcgcc tcgcgcgcga
     4141 tcgcccaggc gctgtagacg atgtagccgg cgatcgcgat cgcgaccagc gggtcgaccc
     4201 aggggacgcc aacatagacg ctcaccccga gcgaggcgat gatcccgcca ttgaccagca
     4261 ggtcggagcg atagtggagc gagtcggcgc tgatcgcggt cgagccggtg cgccgcacga
     4321 catgcatctg gaagcgcacc agggcaaagg tgagcacgat cgagatcacc atcaccgcca
     4381 gcccaacggg gccgtagccg agcgggtgcg gggtgaccag gcgcgcgacc gcctcgacca
     4441 gcacgaacaa cgccgagccg gcgatgaacg ccgcctggcc gaggccggcg atcgactcgg
     4501 ccttgccgtg gccgaagcgg tgctcggggt cggccggcgt cagcgcctgg cggaccgcga
     4561 acaggttgac cagcgaggcg aatgaatcga gcaccgagtc gaccagcgac gacaggatcg
     4621 caaccgaatc ggtcagcacc caggcgccga acttgagcac gatcagcgtc gccgccaccg
     4681 ccaccgaggc atgggtggcg aggcgcagca ggcgcgcatc ctcgcggccg atgggtgctg
     4741 acgcgggcgc gctcacggga acagccgcgc cgtgcgccag ccggcatcgc cggcatcgtc
     4801 gcgctcgtag accagccggt cgtggaggcg gaagttgccg ctgcgccaga actcgatccg
     4861 cagcggcacg atgcggaagc cggaccagaa cggcggccgc ggcacctcgc cgagcccgaa
     4921 cctggccgcg tacttcgcca cccggcgctc gagctcgtag cggctggcca gcggctgcga
     4981 ctgcttgctc gcccacgccc cgatccggct ctggcgcgtg cggctggcga aataggcgtc
     5041 ggccgcgtcg tcggtgaccc ggctcgccgg gccttcgacg cgcacctggc ggcgcagcga
     5101 cttccagtgg aagcacagcg ccgcgcgcgg gttggccttg atctcggcgc ccttgcggct
     5161 gccgaggttg gtgaagaaca cgaatcccgc ggcatcggca cctttgagca acaccatgcg
     5221 tgccgacggg accccgcccg cgcccaccgt ggccaggcat accgcgttgg gatcgttggg
     5281 ttccttggcc acggcctcgc tcagccactc gttgaacaag gccacggggt cgtcgcgcgc
     5341 ccggatcagc atttcgcctg cccgcggttc ttttaccgcc tccgggcact ccctatattg
     5401 tagactcgtg agtgacaagc ccgcggacgt tgggggccgt tcgcccagcc ccgggggccc
     5461 gccaaacccc tgagacgctt gaggattgca gtcatgaaca gtttcaaggc cgtggtcgcg
     5521 gcggggatga tcctgtcgct aggcgcgtgc ttggagggtc aaggcgacaa gcaggtcgcc
     5581 ggcaccctgg tcggcgccgg gctgggcggt cttgccggtt cgcagatcgg cagcggtacc
     5641 ggccagctgg cggcggtcgg cgcgggcgtg ctgctcggcg gcctgctcgg cagcgaggtc
     5701 ggccggtcgc tcgacaaggc cgaccaggct tacgccgcac agacctatca gagcaccctg
     5761 gaatcggtgc ccacgggcca gacccagagc tgggccaacc ccgacagcgg caaccagggc
     5821 acctacaccc cgacccgcac ctatcagacc gaatcggggc agtactgccg cgagttccag
     5881 cagaccatca ccgtcggcgg cgaggtccag tcgggctacg gcaccgcctg ccgccagggc
     5941 gacgggacct ggaagatcgt cagcaactga cgccgcaccg ccggcgcgcc gtaaccccta
     6001 cctgatgcat ggaccctctc ccgggcttat ggcccgagag agggcgccgg cgcgccgccg
     6061 cgtcgggggc cgccattgcg ggatcgacgc cgttagccta aatccgacct ctccgggctc
     6121 gatccgtggc ggcggatcga tcccaaccaa actcggcacg ggtcacccgc acgatgaggg
     6181 atccctacga ggttctgggc gtggcccgcg acgccagcca ggaccagatc aagaaggcct
     6241 accgcaagct cgccaagcag ctgcacccgg acacgcatcc gggtgacacc aaggtcgagg
     6301 atcgcttcaa gcaggtgtcg gcggcctacg ccatcctgtc cgaccccgag atgcgccagc
     6361 gctacgaccg cggcgaggtc gacgccagcg gccaggagcg gccgcagtgg cgcttccgcc
     6421 agccgcacgg ggcggacgcc ggcgccggcg gcttcgccgg cgggttcaac gtcgaggaca
     6481 tcctgggcga cctgttcggc ggccgcttcg gcgccggccg aggggcgatg cgcgcccgcg
     6541 gccgcggcgt gagctacacg gtgcgcatcg gcttcgagga atcggcgcgc ggcggcaagc
     6601 ggcgcgtcac cctgggcggc gggcgcagcc tcgatgtcgc catcccggag gggatcgagg
     6661 acggccagtc gatccggctc aagggccagg gcgagcccgg catcggcggc gggcc
//
LOCUS       DASWHU010000334         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2801152, whole genome shotgun sequence.
ACCESSION   DASWHU010000334 DASWHU010000000
VERSION     DASWHU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801152"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VGB88_12590"
     CDS             <1..723
                     /locus_tag="VGB88_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9648334.1"
                     /translation="LIARFWKSPYRAGLVRWGTGLHDRFMLPHFVWQDLCEVIADMTR
                     AGYALNADWFAPHHEFRFPLYGKIAHGDIALELRQALEPWHVMGEEGAPGGTVRYVDS
                     SVERLQVKATNLAGGRYLIACNGKRVPMVPTGTAGEVVGGVRFRAWQPASSLHPTIGI
                     DAPLVFDLYDRWSGRAVAGCTYHVAHPGGLSFEHFPVNAYEAESRRLARFFAFGHTGG
                     PCPEPADLARPEFPCTLDLRWS"
     gene            744..3338
                     /locus_tag="VGB88_12595"
     CDS             744..3338
                     /locus_tag="VGB88_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9648335.1"
                     /translation="MPETALPLTASPLPPGADLLGSYRPAAGAYDELMAGDGAVRRHW
                     RWFLDAFAGLDDDERAHALEVAQHLLRENGVTYVARGETEHGNRPWQLDLIPLLVEQA
                     EWQRLEAALVQRAQLLNSVLADLYGPQRLLKEGVIPAALVYGNPQFLRPCHGIAVPGG
                     VHLHFVAFDLARAADASWWVLSNRTQAPSGAGYALENRIVASRALPELFAQANVQRLA
                     SFFGAFSEHFLALSRHDEPLAVVLSPGPTKEAYFEHAYLARYLGYTVVEGSDLTVRDD
                     RLYLKTVAGLKVVDLLLRRVPSDMCDPLELNTNSGLGVPGLLQAARAGHVAIANALGS
                     GLVENDALISFLPGLCRFLRGEELIMPSIATWWCGQSRERHYVTQNLDRLVVRKVVSP
                     KTIFAPGRDSYIGPELNEADRDRLAREIARHGHAFIGQEMVSLSTTPTWRDGDGLGPA
                     PMTLRVYLAAGPDGYQVMPGGLTRVALNAPARAPWLGPGDLSKDTWVLWREPVDTFSV
                     FTQPHHELRLKRSGSDLPSRTADNLFWLGRYAERSEGAVRLLRSLVVRLGGEAGSSAD
                     SAMLERLVSLLVIQDHLSQRRARRALDVGIRAVEQEFSNILFDPDSPDGLARVLGNVR
                     RIAELVRERLSHDTWQIIKDLTEITATWRLGEGQELDDARRLLNRMIQLSAALNGMVL
                     ENMTRGYGWRFLDMGRRLERAQHGARLVRHLAAHGDPEATGMLDLLLELADSTMTYRT
                     RYRASPRLHTALDLVLADETNPRSLLFQLMAIDEHLAILPLEARDAGLSPAKRLTTGL
                     CTDLRLADMARLCEVSGKRGLRVNLDRLVRRLEAGVQELSDVVARTYFSHSLTQHISG
                     PHWIEAVP"
     gene            3335..>4011
                     /locus_tag="VGB88_12600"
     CDS             3335..>4011
                     /locus_tag="VGB88_12600"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9648336.1"
                     /translation="MIYALSHRTLFSYSQSVSISHHVLHLAPRESPRQSCLESAIAVD
                     PAPAIRYDDHDYFGNPVTFLTVQEPHPELEVHARSRIEVRAAAGVALDEGPRWEEVAR
                     LAETSREAEPLDAYQYAFDSPYVSCGEETYDFALGSFTPGRPMLAGVIDLTARIFTGF
                     AYQGGVTDVSTPVSEVLKTRTGVCQDFAHVEIACLRSLGLPARYVSGYLLTRPPEGKE
                     RLVGADAS"
BASE COUNT          543 a         1482 c         1370 g          616 t
ORIGIN      
        1 ctgatcgcgc gcttctggaa aagcccctac cgcgccggcc tggtgcgctg gggcaccggg
       61 ctgcacgacc ggttcatgct gccccacttc gtctggcagg acctctgcga ggtgatcgcc
      121 gacatgaccc gcgccggcta cgcgctgaac gcggactggt tcgcgccgca tcacgagttc
      181 cgcttcccgc tctacggcaa gatcgcgcac ggtgatatcg cgctcgagct gcgccaggcg
      241 ctggagccgt ggcacgtgat gggcgaggaa ggcgcccccg gcggcaccgt gcgctacgtc
      301 gattcctcgg tcgagcggct gcaggtcaag gccacgaacc tggcgggcgg ccgctacctg
      361 atcgcgtgca acggcaagcg ggtgccgatg gtgccgaccg gcaccgcggg cgaggtcgtg
      421 ggcggcgtcc ggttccgcgc ctggcagccg gcctcgagcc tgcatccgac catcggcatc
      481 gacgcgccgc tggtgttcga tctgtacgac cgctggtcgg ggcgcgcggt cgccggctgc
      541 acctatcacg tcgcccatcc gggcgggctc agcttcgagc atttcccggt caacgcgtac
      601 gaggcggaga gccgccggct ggcgcggttc ttcgccttcg ggcatactgg cgggccttgc
      661 ccggagccgg cggacctcgc ccggccggag ttcccgtgca cgctcgacct gcgctggtcg
      721 tgagcgccgc tggtttgtcc ccgatgcccg aaaccgccct gccactcacc gcgagcccgt
      781 tgccgcccgg tgccgacctg ctggggtcgt accgtcccgc cgccggggcc tatgacgagc
      841 tgatggcagg cgacggcgcg gtgcgccggc actggcgctg gttcctcgac gctttcgccg
      901 gtctcgacga cgacgagcgg gcgcacgcgc tcgaggtcgc ccagcacctg ctgcgcgaga
      961 acggcgtcac ctacgtcgcc cggggcgaga ccgaacacgg caaccggccg tggcagctcg
     1021 acctgatccc gctgctggtc gagcaggcgg aatggcagcg gctcgaggcg gcgctggtcc
     1081 agcgggcaca gctcctgaac agcgttcttg ccgacctcta cggcccccag cggctgctca
     1141 aggaaggggt gattccggcc gccctggtct acggcaatcc ccagttcctg cgcccgtgcc
     1201 acggcatcgc ggtgccgggc ggcgtgcacc tgcatttcgt cgccttcgac cttgcgcgcg
     1261 ccgccgacgc cagctggtgg gtgctgagca accgcaccca ggcgccgtcg ggcgcgggct
     1321 acgcgctcga gaaccgcatc gtcgcctcgc gcgccttgcc cgagctgttc gcccaggcca
     1381 acgtccagcg cctggcctcg ttcttcggcg cgttcagcga gcatttcctg gcgctgtcgc
     1441 gccacgacga gccgctggcg gtggtgctgt cgccgggccc gaccaaggag gcctatttcg
     1501 agcacgccta tctcgcgcgc tatctcggct acacggtggt cgagggctcg gacctcaccg
     1561 tgcgcgacga ccggctgtac ctgaagacgg tcgcgggcct aaaggtcgtc gacctgttgc
     1621 tgcgccgcgt gccgtccgac atgtgcgacc cgctcgagct caacaccaac tcgggcctcg
     1681 gcgtgcccgg cctgttgcag gcggcgcgcg ctggccacgt cgccatcgcc aacgcgctcg
     1741 gcagcggcct ggtcgagaac gacgcgctga tcagcttcct gcccggcctg tgccggttcc
     1801 tgcgcggcga ggagctgatc atgccgagca tcgccacctg gtggtgcggc cagtcgcgcg
     1861 agcgccacta cgtgacgcag aacctggacc ggctggtggt gcgcaaggtg gtgtcgccga
     1921 agaccatctt cgcccccggc cgcgacagct atatcggccc cgagctgaac gaggcggacc
     1981 gcgaccggct ggcgcgcgag atcgcccgcc atggccatgc attcattggc caggaaatgg
     2041 tttccctgtc gaccacgccg acctggcgcg acggcgacgg cctgggcccg gcgccgatga
     2101 ccttgcgagt ctatctcgcc gccggccccg acggctacca ggtgatgccg ggcgggctca
     2161 cccgagtcgc gttgaacgcg ccggcgcggg cgccgtggct cgggccgggc gacctcagca
     2221 aggacacctg ggtgctgtgg cgggagccgg tcgacacctt cagcgtgttc acccagccgc
     2281 atcacgagct caggctcaag cgcagcggca gcgacctgcc gagccgcacc gccgacaacc
     2341 tgttctggct cggccgctac gccgagcgct cggagggcgc ggtgcgcctg ttgcgcagcc
     2401 tggtcgtcag gctcggcggc gaggccggat cgagcgccga ttcggcgatg ctcgagcggc
     2461 tggtctcctt gctcgtgatc caggatcacc tgtcccagcg gcgcgcgcgg cgcgcgctcg
     2521 acgtgggcat ccgcgcggtc gagcaggagt tctccaatat cctgttcgac cccgattcgc
     2581 ccgacggcct cgcccgggtg ctcggcaacg tccgccgcat cgccgagctg gtgcgcgagc
     2641 gcctgtcgca cgatacctgg cagatcatca aggacctgac cgagatcacc gccacctggc
     2701 ggctcggcga gggccaggag ctcgacgacg cccggcgcct gctcaaccgc atgatccagc
     2761 tttcggcggc gctcaacggc atggtgctcg agaacatgac ccgcggctac ggctggcgct
     2821 tcctcgacat gggccggcgc ctggagcgcg cccagcacgg cgcgcggctg gtgcgccacc
     2881 tcgccgccca cggcgacccc gaagcgaccg gcatgctcga cctgctgctc gagctcgccg
     2941 acagcaccat gacctaccgc acgcgctacc gcgcgagccc gcgcctgcac accgccctcg
     3001 acctggtgct ggccgacgag accaacccgc gctcgctgtt gttccagctg atggcgatcg
     3061 acgagcatct cgccatcctg ccgctggagg cgcgcgatgc cgggctcagc ccggccaagc
     3121 gcctgaccac cggcctgtgc accgacctca ggctcgccga catggcgcgc ctgtgcgagg
     3181 tgagcggcaa gcgggggctc agggtcaacc tcgaccggct ggtgcgccgc ctcgaggctg
     3241 gcgtgcagga gctgtccgac gtggtcgcgc gcacctattt cagccactcg ctgacccagc
     3301 acatctccgg cccccattgg atcgaggcgg tgccgtgatc tacgcgctca gccatcggac
     3361 gttgttctcg tactcgcagt cggtgtcgat ctcgcaccac gtcctgcacc tcgcgccgcg
     3421 cgagagcccg cgccagtcgt gcctcgagtc ggcgattgcg gtcgatccgg cgccggcgat
     3481 ccgctacgac gaccacgact acttcggcaa cccggtgacc ttcctcaccg tccaggagcc
     3541 gcacccggag ctcgaggtgc acgcgcgctc gcgcatcgag gtgcgcgccg cggccggggt
     3601 cgcgctcgac gagggcccgc gctgggaaga ggtggcgcgg ctcgccgaga cctcgcgcga
     3661 ggccgagccg ctggatgcct atcagtacgc cttcgattcg ccctacgtga gctgcggcga
     3721 ggaaacctac gacttcgcac tcggctcgtt caccccgggc cggccgatgc ttgccggcgt
     3781 gatcgacctg acggcgcgca tcttcacggg cttcgcctat cagggtggtg tcaccgacgt
     3841 ctcgaccccg gtcagcgagg tgctcaagac gcgcaccggc gtgtgccagg acttcgccca
     3901 tgtcgagatc gcctgcctgc gcagcctcgg cctgccggcg cggtatgtca gcggctacct
     3961 gctgacccgc ccgcccgagg gcaaggagcg gctggtcggc gccgacgcct c
//
LOCUS       DASWHU010000335         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3182519, whole genome shotgun sequence.
ACCESSION   DASWHU010000335 DASWHU010000000
VERSION     DASWHU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3182519"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VGB88_12605"
     CDS             complement(<1..878)
                     /locus_tag="VGB88_12605"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX9648337.1"
                     /translation="MTLTRRALAAAAVLGLAALPAAAQVEVRVYPPPPPAVSPIAGAI
                     DFHVHSAPDVFGRNLLDVEVARTAARAGMRALVLKNHVTSSADRAYLAMREVPGIEVF
                     GGVVLNRAVGGINPDAVEWMYRMEGGRGKVVWLPSFDADFHLRTFGDPGDGLKVAIDG
                     VVTAEMEAVLKIVARERLVLCTSHVSPDEALAVIRRGRDLGVENMVVTHAMADVPGMT
                     LGEMQQAAAMGAYLELVYLNDLMGPNAHLAWMRHWTRVSTADMAAAIRAVGAAAFILS
                     TDLGQTGNPIHPDGYKT"
     gene            1004..>2733
                     /locus_tag="VGB88_12610"
     CDS             1004..>2733
                     /locus_tag="VGB88_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9648338.1"
                     /translation="MSQDQAFAFAILLGALALFAWGRLRYDVVAALALGAAVALGLVP
                     GADAFAGFGHPAVITVALVLMISHGLKQSGLVERMAGMLAPLTGPLLLHIAALTAVVA
                     VASAFMNNVGALALMLPVALATAAERKRSPALLLMPLAFGSILGGMTTMIGTPPNVII
                     ATFRADYAGAPFAMFDFSPVGVAVAAVGVVFVALVGWRMIPAERRAQNPPQQLFAIDD
                     YLTEIEVGKSSPLVDERLEETKAFDDAAVDVIGLIRHGSDRPISVPRQPLKAGDVLLV
                     RGDPEALEPLMAKLDLKLAQKPSAHLKGDELTLAEGVVSPGAALEGRGLRYLRRRTGG
                     ALAVVALARQGRPPRGRLTGQTFRAGDVLLLHGQGDALDDWFATLGLLPLAARGLSLG
                     QKRRMGLALGVFAAAILTMTLGLAPAPVALAFAAIAYVLLDVLPLRDLYREIDWPVVV
                     LLGAMIPVGGALQETGAAALAADAVARLSAGAGPLLVLAFVLVLAMFLSDVINNVATA
                     LVMAPIAIGIADRLDANPDSFLMAVAVGSSCAFLTPIGHQSNTLVMGPGGYRFGDYWR
                     MGLPLEAAIVA"
BASE COUNT          331 a          975 c          995 g          432 t
ORIGIN      
        1 gtcttgtagc cgtcggggtg gatcggattg ccggtctggc cgagatcggt gctgaggatg
       61 aacgccgcgg cgccgaccgc gcggatcgcc gccgccatgt cggcggtcga gacccgcgtc
      121 cagtggcgca tccaggcgag atgcgcgttc gggcccatca ggtcattgag gtagaccagc
      181 tcgagatagg cgcccatcgc cgccgcctgc tgcatctcgc caagcgtcat gccgggcacg
      241 tcggccatgg cgtgggtgac gaccatgttc tcgaccccga ggtcgcggcc gcggcggatc
      301 accgccaggg cctcatccgg tgagacgtgg ctggtgcaca gcaccagccg ctcgcgggcg
      361 acgatcttga gcaccgcctc catctccgcg gtgaccacgc cgtcgatcgc gaccttgagc
      421 ccgtcgccgg ggtcgccgaa ggtgcgcagg tggaaatcgg cgtcgaacga gggcagccac
      481 accaccttgc cgcgaccgcc ctccatccgg tacatccact cgaccgcgtc cgggttgatg
      541 ccgccgaccg cccggttgag cacgacgccg ccgaacacct cgatcccggg cacctcgcgc
      601 atcgccaggt aggcgcggtc ggcgctgctg gtgacgtggt tcttgagcac gagtgcgcgc
      661 atgcccgcgc gggccgcggt gcgcgcgacc tcgacgtcga gcaggttgcg cccgaacacg
      721 tccggcgccg aatggacgtg gaagtcgatg gcgcccgcga tcgggctcac cgccggcggc
      781 ggcggcgggt agacgcgcac ctccacctgg gccgcggccg gcagcgcggc aagccccagc
      841 actgccgccg cggccagtgc ccgccgtgtc aaggtcatcg gatcctccct ccaaagccgc
      901 gcctgcgcgc gccccgcggg cgatgctacg tccgggcgat gcgcgttgca aaccggcgcg
      961 gcgcctgtgg cggcgcgtac cggcggcatg ctaagtccgc gcgatgagcc aggatcaggc
     1021 gttcgctttc gcgatccttt tgggtgcgct ggcgctgttc gcctgggggc ggctgcgcta
     1081 cgacgtggtc gcggcgctcg cgctgggcgc ggcggtggcg ctcggcctgg tgccgggcgc
     1141 ggacgcgttc gccggtttcg gccatccggc ggtgatcact gtggcgctgg tgctgatgat
     1201 cagtcacggc ctcaagcagt cggggctggt cgagcggatg gcggggatgc tcgcgccgct
     1261 caccggcccg ctgctgctgc acatcgcggc gctcacggcc gtggtcgcgg tggcgagcgc
     1321 attcatgaac aatgtcggcg cgttggcgct gatgctgccg gtggcgctgg cgaccgcggc
     1381 ggagcgcaag cgttcgccgg cgctgctgct catgccgctc gccttcggca gcatccttgg
     1441 cggcatgacc acgatgatcg gcaccccgcc caacgtgatc atcgccacct tccgcgccga
     1501 ctacgccggg gcgccgttcg ccatgttcga cttcagcccg gtcggcgtgg cggtggcggc
     1561 ggtcggcgtc gtcttcgtcg cgctggtcgg ctggcggatg attcccgcgg agcgccgcgc
     1621 gcaaaatccg ccgcagcagc tgttcgcgat cgacgactat ctgaccgaga tcgaggtcgg
     1681 caagtcgagc ccgctggtcg acgagcggct ggaggagacg aaggcgttcg acgacgccgc
     1741 ggtcgacgtt atcggcctga tccgccacgg ctcggaccgc ccgatatcgg tgccgcgcca
     1801 gccgctcaag gccggcgacg tgctgctggt gcgcggtgac ccggaggcgc tcgagccgct
     1861 gatggccaag ctcgacctca agctggcgca gaagccgtcc gcccacctca agggcgacga
     1921 gctgactctc gctgagggcg tggtcagccc cggtgcggcg ctggagggac gggggctgcg
     1981 gtatctcagg cggcgcaccg gcggcgcgct cgccgtggtt gcactcgccc gccagggccg
     2041 gccgccgcgc ggccggctca cgggccagac cttccgcgcc ggcgacgtgc tgctgctcca
     2101 cggccagggc gatgcgctcg acgactggtt cgccaccctc ggcctcctgc cgctcgccgc
     2161 gcgcgggctc agcctcggcc agaagcgccg catggggctc gcgctcgggg tgttcgccgc
     2221 cgcgattctg actatgacgc tcgggctcgc gccggcgccg gtcgcgctcg cgttcgcggc
     2281 gatcgcttac gtcctgctcg acgtgctacc gctgcgcgac ctctaccgcg agatcgactg
     2341 gccggtggtc gtcttgctcg gcgcgatgat cccggtgggc ggcgcgttgc aggagaccgg
     2401 cgccgcggcg ctcgccgccg atgcggtcgc gcgcctctcc gcgggcgccg ggccgctcct
     2461 ggtgctcgcc ttcgtgctgg tgctggccat gttcctgtcc gacgtgatca acaacgtcgc
     2521 caccgcgctg gtcatggcgc cgatcgcgat cggtatcgcc gaccgcttgg acgccaaccc
     2581 ggattcgttc ctgatggcgg tcgcggtcgg ctcgagctgc gcctttctca cgcccatcgg
     2641 gcaccagtcg aacacgctgg tgatggggcc gggcggctat cgcttcggcg actactggcg
     2701 gatgggcctg ccgctggagg ccgcgatcgt cgc
//
LOCUS       DASWHU010000336         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_947359, whole genome shotgun sequence.
ACCESSION   DASWHU010000336 DASWHU010000000
VERSION     DASWHU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947359"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VGB88_12615"
     CDS             <1..1090
                     /locus_tag="VGB88_12615"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9648339.1"
                     /translation="DIAAMPRSFLFARAWAQWYGGLAIVVLAVALALVPGAVAKRIAF
                     EGEAGDLLGGAHARARRLLGVYAVLTVAAAAALWLLGVPAFDAVTHALSAVSTGGFST
                     RADSAAGLGGGAARAALALACVAGAISFALQYRVSREGPGPLWRDREVRALALVALAG
                     FGALVLVRIAAGAGATPGGVGGAAFLALSAQTTAGFSTAPVAGLDGASKLVLMVQMAI
                     GGDSGSTAGGIKIIRFLVALRLVQALFARTCLPAHAVFEPHYAGRALTPAEVQVIAGM
                     VLLYLGVALVSWLAFLLGGLAPLDSLFEVVSALGTVGLSAGVTGPDLAPALKLVLCAD
                     MLMGRLELVAVLVLLYPRTWLGRRAERA"
     gene            1087..1746
                     /locus_tag="VGB88_12620"
     CDS             1087..1746
                     /locus_tag="VGB88_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9648340.1"
                     /translation="MRAVIIGAGNLGAAVAAGLAERGQDVIMVESDNQRIAALTGTLD
                     CAFIHGDGSRPSILGETGPAKSDVLLCLTGNDQVNIIATLVGRSLGFARIITKIEDPE
                     YEHLCVELAFTDVIIPDRHVARTLADLVSGTLAEDYSTFFKGAARLFSFAARAEDAGE
                     VEALELPADSRVICLYRDDRLVIAKPGAAIRAGDQVVVLATDAALAKLRDRFAAEGGR
                     G"
     gene            1799..>2439
                     /locus_tag="VGB88_12625"
     CDS             1799..>2439
                     /locus_tag="VGB88_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648341.1"
                     /translation="MTGLDLRALAWLTGALAIAGAASGCVVDPTLGIAGAAVNGASYV
                     ATGRSADSHGLSWLTGEDCDLARALSRAPVCRPVGSGDGTIGLAAADGEPERTALAAL
                     YEQAALAPVLVGAAAATAYSEPVPPRLYVVAASYDRRAEATAAAYTLGDLPAAVARTE
                     VAGAVRHRVVVGPLEPRLEAVLGPRLAAAGAEHWFPVALCPPALTPPPCLGPAA"
BASE COUNT          243 a          916 c          931 g          349 t
ORIGIN      
        1 cgacatcgcg gcgatgccgc ggagcttcct gttcgcgcgc gcctgggcgc aatggtacgg
       61 cgggctcgcc atcgtggtgc tggcggtggc gctcgccctg gtgccgggcg cggtcgccaa
      121 gcgcatcgcc ttcgagggcg aggccggcga cctgttgggc ggggcgcatg cgcgcgcgcg
      181 ccgcctgctc ggggtctacg ccgtgctcac cgtcgccgcg gcggcggcct tgtggctgct
      241 cggggtgccc gcgttcgacg ccgtgaccca cgcgctgtcg gcagtctcga ccggcggctt
      301 ctcgacccgc gccgacagcg ccgcggggct cggcggcggc gcggcgcggg cggcgctggc
      361 gctcgcgtgt gtcgccggcg cgatctcgtt cgcgctccag taccgagtct cgcgcgaggg
      421 ccccgggccg ctgtggcgcg accgcgaggt gcgggcgctg gcgctggtcg cgctcgccgg
      481 tttcggcgcg ctcgtgctcg tgcgcatcgc ggcgggcgcg ggcgccacgc ccggcggcgt
      541 cggcggtgcc gcgttcctcg cgctctcggc ccagaccacc gcgggcttca gcacggcgcc
      601 cgtggccggg ctcgacggcg cctccaagct cgtgctcatg gtccagatgg cgatcggcgg
      661 cgacagcggc tcgaccgcgg gcggcatcaa gatcatccgc ttcctggtcg cgctgcgcct
      721 ggtccaggcg ctgttcgcgc gcacctgcct gccggcgcat gcggtgttcg agccgcacta
      781 tgccggccgc gcgctcacgc ctgccgaggt gcaggtgatc gccggcatgg tgctgctcta
      841 cctcggcgtg gcgctcgtct cgtggctcgc cttcctcctc ggcgggctgg cgccgctcga
      901 ctcgctgttc gaggtggtct cggcgctcgg cacggtcggc ctgtccgcgg gcgttaccgg
      961 tcccgacctc gcgcccgccc tcaagctggt gctgtgcgcg gacatgctga tgggcaggct
     1021 tgagctggtg gccgtgctcg tgctgctgta cccgcgcacc tggctcggca ggagggccga
     1081 acgcgcatga gggcggtgat catcggcgcc ggcaacctgg gcgcggcggt ggcggccggc
     1141 ctcgccgagc gcggccagga cgtgatcatg gtcgagtccg acaaccagcg catcgccgcg
     1201 ctcaccggca ccctcgactg cgccttcatc cacggcgacg gcagccggcc gagcatcctc
     1261 ggcgagaccg ggccggcgaa gagcgacgtg ctgctgtgcc tgaccggcaa cgaccaggtc
     1321 aacatcatcg ccaccctggt cggacgctcg ctcggcttcg cccgcatcat caccaagatc
     1381 gaggaccccg agtacgagca cctgtgcgtg gagctcgcct tcaccgacgt catcatcccc
     1441 gaccggcacg ttgcgcgcac gctcgccgac ctggtctcgg gcacgctcgc cgaggactac
     1501 tcgaccttct tcaagggcgc ggcgcggctg ttctcgttcg cggcgcgcgc ggaggacgcg
     1561 ggcgaggtcg aggcgctcga gcttcccgcc gacagccggg tgatctgcct ctaccgggac
     1621 gaccggctgg tgatcgccaa gccgggggcg gcgatccgcg ccggcgacca ggtggtcgtc
     1681 ctcgccaccg acgccgccct ggccaagctg cgcgaccgct tcgccgccga gggcgggcgc
     1741 ggctaggagg cgggcgccgg cggcggaaac gcggcgcgcg ggcgctatat cgacagtcat
     1801 gaccggcctc gaccttcgcg ccctcgcttg gctcaccggc gcgctcgcaa tcgcgggcgc
     1861 cgcgtcgggg tgcgtggtcg acccgaccct cggcatcgcc ggcgccgcgg tcaacggcgc
     1921 gagctatgtc gccaccggca ggagcgcgga ctcccacggc ctgtcgtggc tcaccggcga
     1981 ggactgcgac ctggcgcggg ccctgtcgcg cgcgcccgtg tgccggccgg tgggcagcgg
     2041 cgacggcacg atcggcctgg cggcggcgga cggcgagccg gagcgtaccg cgctggccgc
     2101 gctctacgag caggcggcgc tagcgccggt gctggtcggt gccgccgcgg ccacggccta
     2161 ttccgagccg gtgccgccgc ggctgtacgt ggtcgcggcg agctatgacc ggagggccga
     2221 ggcgaccgcc gccgcgtata cgctcggcga cctgccggcg gcggtggcac ggaccgaggt
     2281 cgcgggcgcg gtccgccacc gggtggtggt cggcccgctc gagccgcgcc tggaggcggt
     2341 gctcggcccg cgcctcgccg ccgccggcgc cgagcactgg ttcccggtcg cgttgtgccc
     2401 gccggcgctg accccgccgc cgtgcctcgg cccggccgc
//
LOCUS       DASWHU010000337         9938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_950611, whole genome shotgun sequence.
ACCESSION   DASWHU010000337 DASWHU010000000
VERSION     DASWHU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950611"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VGB88_12630"
     CDS             <1..711
                     /locus_tag="VGB88_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9648342.1"
                     /translation="LDAELVEIVARSLAVSLSAVVLAAAVGLPLGAAVAVYRFPGRRA
                     LVVLLNALMGLPPVFVGLMLYVLLSRAGPLGFLGLLFTPTAMVIAQAVLITPIIAALS
                     HRVLRDLYEGYREQLCSLGARPWDAVGTLLWDARVSLVTVVMAGFGRASAEVGAVLIV
                     GGNIAHLTRVMTTTIALETSKGNLALALALGLILLLVAVGVNAAAAVIGGVRFGPAAQ
                     RLDRPSVGRSRLSDGEGR"
     gene            708..1412
                     /locus_tag="VGB88_12635"
     CDS             708..1412
                     /locus_tag="VGB88_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648343.1"
                     /translation="MTILPLMLDEAVVSAGAHRLLGPLTLSLEAGPRTVILGANGAGK
                     SLLLRLCHGLIPPSAGRVRWHGADAFRRQAMVFQQPVMLRRSVRANVAYGLGLRAVAR
                     AEREPRIAAALAAAGLAPLADRPAPVLSAGEQQRLALARVWALAPEVLFLDEPTANLD
                     PGATGAVERIVDALHAAGCKIIMTTHDMNQARRLADEVIFLHGGVVVERGEAARFFDR
                     PQTETATAFLEGRLLW"
     gene            1415..2245
                     /locus_tag="VGB88_12640"
     CDS             1415..2245
                     /locus_tag="VGB88_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9648344.1"
                     /translation="MHSGIRGRVASLVVAAALVAQPAAADRAIVLASTTSTEVSGLFA
                     HILPPFEAETGIAVRVVAVGTGQALKLAQNGDADVLLVHHRPSEEAFVAEGYGVERFD
                     VMYNDFVLVGPGADPAGIKGLAGAAEALARIAASHAPFASRGDDSGTHKAERALWQAA
                     GVDPAAASGGWYRELGQGMGATLNAAQAMDAYTLSDRATWLAFENKGSLEILVEGDPA
                     LFNQYGVILVNPARHPHVDAAGGQAFIDWLLSPAGQGAIAGFHISGAQLFFPNAKARR
                     "
     gene            2352..2966
                     /gene="rpsD"
                     /locus_tag="VGB88_12645"
     CDS             2352..2966
                     /gene="rpsD"
                     /locus_tag="VGB88_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9648345.1"
                     /translation="MSKRKTSKYKLDRRLGVNLWGRPKSPINLREYGPGEHGQRRRKV
                     SDYGIQLAAKQKLRGYYGDLSERQFRRTYQEAIRRSGDTGENLVGLLERRLDAVVYRM
                     GLAPTVFSARQIVNHGHIRVNGRRVTIPSYLVNEGDVLEVHEKSKGHPLILETIQQPE
                     RDVPDYIEVDFDKLKGTFLRQPQLADIPYAVQMEPNLVIEYYSR"
     gene            complement(3318..4532)
                     /locus_tag="VGB88_12650"
     CDS             complement(3318..4532)
                     /locus_tag="VGB88_12650"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9648346.1"
                     /translation="MADAGAKPRPLDGIRVIDAATFIAAPFCATLLGEFGAEVIKVEL
                     PGTGDPARHFGSATPCGDSLVWLNEARNKKSITLDLRTADGAALFKRLAAQADVVCEN
                     FRAGTLERWGLGYEDLRAHNAGLVMVRITGYGQTGPYAARPGFGRIGNAFGGLSFLAG
                     HPDREPVTPGSATLADYVSGLFGAFGALLALRAREATGTGQVVDIGLYESIFRLLDEL
                     VPAYAMFGKVRQRIGAGTVNAVPHSHYPTRDGTWVAIACTSDKMFARLAALMGRPELA
                     GDGRLATVARREAAREEVDRMVADWTRAHGRAELLAACERAEVPCGPVYAIDEIFADP
                     QYRARGNLRTIEDSRAGPVTVAEVVPRLSETPGAIDWLGPALGAHLAEVCGRLLGLDE
                     AEVAALKRRGVL"
     gene            complement(4554..5771)
                     /locus_tag="VGB88_12655"
     CDS             complement(4554..5771)
                     /locus_tag="VGB88_12655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9648347.1"
                     /translation="MSRTSRLVTQGFAGVAHSFAHLFVLLFATVVLVLEREWGMDYAS
                     LFALGIPMSILFGAGALPAGWLGDRWSETGMMTVYFFGLGAATILTGFADGPASLSGG
                     LALMGLFASIYHPVGIPWLVRHAPNRGRALGINGVFGSLGTAAAALVAGTLASYLSWR
                     WAFFVPGAVCMSAGIVFVFARRRGLFEATSTATSRISGERPAEGARRVFTVLAVTVLC
                     TGMIYQMTSFALPKIFAERLGALFGDGVVGVAGMVTLVYLASSLTQIVGGEFADRFRL
                     KTVYLTTQFLQVPVLLVAFALVHPALVPVAALMIGLNVAGQPAENALLARYTPAHWRG
                     RVFGAKFMLTLGVSSFGVALIPIVYRMTGSLDLLFLAMTVLALAAGTAALRLPAGEGQ
                     AAPAPLPSRAPAE"
     gene            complement(5821..7146)
                     /locus_tag="VGB88_12660"
     CDS             complement(5821..7146)
                     /locus_tag="VGB88_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008756818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9648348.1"
                     /translation="MGVEIPIGWLTLLMFGSLLLLLMAGLPLAFVTGGLACVFLFVLN
                     DTQMLNMVPSRIFPLMTDYQLSAIPLFIFMAAMLERAGLIGEMFDVIYKLLGGIRGGL
                     ASATIVASTILAAMVGIIGAAVVTMGIIALPAMLKRNYAPKIAMGAIMAGGTLGILIP
                     PSILAIIYAVVAEQSVGELFIGSVIPGLMLSGMYIAYVSLRCWVSPEMGPALPLAERI
                     GTRDKLRLVGTMTAPILLIVLVLGVIFTGIATPVEAAGLGTFGSFVVAALHRRLDWLT
                     VREACITTLKATSMVLWIMFGATVFVGLYVLQGGQEFVTGALEATGLGRWGILILMQL
                     ILIVLGMFLDWVGILLLCVPIFVPIIKGLGFDPLWFGVLYLVNMQMSFLSPPFGYALF
                     YLRGVAPPDIPMADIFKAALPFLAIQATGLAACMMYPPLITWLPRLVYG"
     gene            complement(7150..8238)
                     /locus_tag="VGB88_12665"
     CDS             complement(7150..8238)
                     /locus_tag="VGB88_12665"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9648349.1"
                     /translation="MPDLTFVLPHWLYWSGLAAFPLLAMALVRRQRLAGPRPAVTLAV
                     GYLLLITAGFAGIHRFYVKNWRGVIYLPFVVAIVVANIESRASREVISAAESQVMIAQ
                     FDVDRARAAVDAGDEDAGAKLEAGRAELTRAEAAKGAAAATFDQWAGIARTVAIVIGI
                     LLLFDAIRLPALARLCNQREPAIPVPEPVAPPVAPARPASIVRHFETLSRWTGEYVAY
                     WSVIAVFAYYYEVVARYLFNSPTNWVHESMFLMFGMQYLIAGAYAYLTDSHVRVDIFY
                     ARLSRRGRAATDLLTSVFFFIFAGTLLATGWVFLMDSVGVWEVSFTEWAIQYWPVKIT
                     IAVGAGLILIQGLAKMLRDLAILVAREA"
     gene            complement(8334..9503)
                     /gene="dctP"
                     /locus_tag="VGB88_12670"
     CDS             complement(8334..9503)
                     /gene="dctP"
                     /locus_tag="VGB88_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9648350.1"
                     /translation="MTESTTTGRDKRPAARARGASRRAVLKGAVGAAGAAAAFAAADR
                     LGAPFIGTARAQTATWKIQTSWPGGIGLDIFRSWCSTIVEKTGGELAFQPFGAKDVVG
                     DFQLFDAVKNGVLDAMNPFTIYWAGRMPASVFLTSYPLGPRYPHEWDVFYYGLGGLEI
                     ARELFAKQDMYYVGPIQHGPNIIHSKVPIRSIEDFRGRKMRLPGGMVAEVFQAAGAKT
                     TLLPGSEIFPALEKGTIDVADYVGPAVNYALGFHQVTKFISMGPPGFMSLYQPVDLMD
                     LTVSNRAWNALSPQMRQFVEMEVHVYSDMHHAAIQKADQESWAKFAEAGTVVTRLTEA
                     DVEKFTRLAIPRWFEWANKDQDAARVFKIQLDYMMSGSLGYVTPDMIKGQELKWS"
     gene            complement(9605..>9938)
                     /locus_tag="VGB88_12675"
     CDS             complement(9605..>9938)
                     /locus_tag="VGB88_12675"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9648351.1"
                     /translation="TDGRFSGATSGLSVGHVSPEAADGGELALIEDGDEIAFDIPARS
                     IRLAVDDAALARRRTAMAARADGGWKPVNRARIVSRALRAYGAMVRSASFGAVRDPAV
                     LNGAGPGA"
BASE COUNT         1563 a         3522 c         3498 g         1355 t
ORIGIN      
        1 ctcgacgccg agctggtcga gatcgtggcg cgctcgctcg cggtcagcct gagcgcggtg
       61 gtgctcgccg ccgcggtcgg cctgccgctc ggcgccgcgg tcgcggtcta ccggttcccc
      121 ggccggcgcg cgctggtggt actgctcaac gccctgatgg ggctgccgcc ggtgttcgtc
      181 ggcctgatgc tgtacgtgct gctgtcgcgc gccggcccgc tcggcttcct cggcctgctg
      241 ttcaccccga ccgcgatggt gatcgcgcag gcggtgctga tcaccccgat catcgccgcg
      301 ctgtcgcacc gggtgctgcg cgacctctac gagggctatc gcgagcagct gtgctcgctc
      361 ggcgcgcgcc catgggacgc ggtcggcact ctcttgtggg acgcgcgcgt cagcctggtg
      421 acggtggtga tggccgggtt cgggcgcgcc agcgccgagg tcggcgcggt gctgatcgtc
      481 ggcggcaaca tcgcgcacct cacccgcgtg atgaccacca ccatcgcgct cgagaccagc
      541 aagggcaacc tcgcgctggc gctcgctctc ggcctgatcc tgctcttggt ggcggtaggc
      601 gtgaacgccg cggcggcggt gatcgggggc gtgcggttcg gtcccgccgc gcagcggctc
      661 gaccgacctt cagtcggccg aagccgactt tcggacggcg aaggccgatg acgatcctgc
      721 cgctgatgct cgacgaggcg gtggtgagcg cgggcgcaca ccgcctgctg gggccgctca
      781 cgctcagcct ggaggccggc ccgaggaccg tgatcctcgg tgccaacggc gccggcaaga
      841 gcctgctgtt gcgcctctgc cacgggctga tcccgcccag cgcgggccgc gtgcgctggc
      901 acggcgccga cgccttccgg cgccaggcga tggtgttcca gcagccggtg atgctgcgcc
      961 gctcggtgcg cgccaacgtc gcctacggcc tcggcctgcg cgctgtggcg cgcgccgagc
     1021 gcgagccgcg catcgccgcc gcgctcgcgg ccgccggcct cgccccgctc gccgaccgcc
     1081 ccgccccggt gctgtcggcg ggcgagcagc agcggctcgc gctggctcgg gtgtgggcgc
     1141 tcgcgcccga ggtgctgttc ctcgacgagc cgaccgccaa cctcgatccg ggcgcgaccg
     1201 gcgcggtcga gcggatcgtc gatgccttgc acgccgccgg ctgcaaaatc atcatgacca
     1261 cgcacgacat gaaccaggcg cgccggctcg ccgacgaggt catcttcctg cacggcggcg
     1321 tggtggtcga gcgcggcgag gccgcgcgct tcttcgaccg gccgcaaacc gagaccgcaa
     1381 ccgcgttcct ggaaggacga ttgctatggt gagcctgcat tcaggcattc gtggccgggt
     1441 cgcgtcgctg gtcgtggcgg cggctctcgt tgcgcagccg gcggcagccg accgcgcgat
     1501 cgttctcgcc tcgaccacct cgaccgaggt ctcgggcctg ttcgcccaca tcctgccgcc
     1561 gttcgaggcc gagaccggca tcgccgtgcg cgtggtcgcg gtcggcaccg gccaggcgct
     1621 caagctggcc cagaacggcg atgccgacgt gctgctggtc caccaccgcc cgtccgagga
     1681 ggcgttcgtc gccgagggct acggcgtcga gcgcttcgac gtcatgtaca acgacttcgt
     1741 gctggtcggc cccggcgccg acccggccgg catcaaaggc ttggcgggcg cggccgaggc
     1801 actggcgcgg atcgccgcct cgcacgcccc gttcgcctcg cgcggcgacg acagcggcac
     1861 ccacaaggcc gagcgcgcgc tgtggcaggc ggcgggcgtc gatccggccg cggccagcgg
     1921 cggctggtac cgcgagctcg gccagggcat gggcgcgacg ctcaatgccg cccaggcgat
     1981 ggacgcctat accctcagcg accgcgccac gtggctcgcg ttcgagaaca agggcagcct
     2041 cgagatcctg gtcgaaggcg atccggcgct gttcaaccag tacggggtga tcctggtcaa
     2101 ccccgcgcgc catccgcacg tcgatgcggc gggcggccag gcgttcatcg actggctctt
     2161 gtcgccggcc gggcaggggg cgatcgccgg ctttcacata agcggcgcgc agctgttctt
     2221 tcccaacgcc aaggcccggc gctgaggcgc ttacggtttg acagacccgg ccgcgccgat
     2281 atagtacgcg gcttcagttt cgcgggcggc cgccggcggc gcgccgcccg cgccttctac
     2341 tcggatcacg gatgagcaag cgcaagacct cgaagtacaa gctcgaccgc aggctggggg
     2401 tcaacctctg gggccggccc aagagcccga tcaacctgcg cgagtacggt cccggcgagc
     2461 acggccagcg ccgccgcaag gtgtcggatt acggcatcca gctggcggcc aagcagaagc
     2521 tcaggggcta ttacggcgac ctgtcggagc ggcagttccg gcgcacctac caggaagcga
     2581 tccggcgcag cggcgatacc ggcgagaacc tggtcggcct gctcgagcgc aggctcgatg
     2641 cggtggtcta ccgcatgggc ctggcgccga ccgtcttctc cgcccgccag atcgtcaacc
     2701 acggccacat ccgggtcaac ggccggcggg tgacgatccc gagctacctg gtcaacgagg
     2761 gcgacgtgct cgaggttcac gagaagtcga agggccaccc gctgatcctg gagaccatcc
     2821 agcagcccga gcgcgacgtt cccgactaca tcgaggtcga cttcgacaag ctcaagggca
     2881 ccttcctgcg ccagccgcag ctcgcggaca tcccctatgc ggtgcagatg gagcccaatc
     2941 tggtgatcga gtactactcg cgctgagccg cgggcagcgg tgggggacag cgcgaccggc
     3001 cgccccgcgg ggcggccttc ttttttctat gcagtcccag gtcgggcctt cgccacagga
     3061 catacagggt cgtcatcgcg agcggagcga agcgatccag aggcggcgcg ggccgaaccg
     3121 aagactggat cgcctgcctg cgcgaagccg accttccttc gcccgccgaa gcgggcttcg
     3181 cgaaggcggg agcggcttcg cttcggcccg tcgcccgaag gctttgggcc gcagggcggc
     3241 gaaggcaggc cacggcgctg cgcgcctcgc gatgaccgcg ttctgcgcgc cctcaccgtc
     3301 cttgtcgttc aattacctca taagactccg cggcgcttga gggctgcgac ctcggcctcg
     3361 tccaggccta gcagccggcc gcagacctcg gccagatgcg cgccgagcgc cgggccgagc
     3421 cagtcgatcg cgcccggggt ctcgctcagg cgcggcacca cctcggcgac ggtgaccggg
     3481 cctgcgcgcg aatcctcgat ggtcctgagg ttgccgcgtg cgcggtattg ggggtcggcg
     3541 aagatctcgt cgatcgcgta gaccggcccg cacggcacct cggcgcgttc gcacgccgcg
     3601 agcagctcgg cgcgaccgtg ggcgcgggtc cagtccgcga ccatgcggtc gacctcctcg
     3661 cgcgccgcct cgcgccgagc cacggtggcg aggcggccat cgccggcgag ctcgggccgg
     3721 cccatcagcg cggcgaggcg cgcgaacatc ttgtcgctgg tgcaggcgat cgcgacccac
     3781 gtgccgtcgc gggtcgggta gtggctgtgg ggcaccgcgt tcaccgtgcc cgcgcctatg
     3841 cgctggcgca ccttgccgaa catcgcgtag gccgggacga gctcgtcgag caggcggaag
     3901 atcgactcgt acaggccgat gtcgaccact tggccggtgc cggttgcctc gcgcgcacgc
     3961 agcgcgagca gcgcaccgaa cgcgccgaac aggcccgaga cgtagtcggc gagcgtcgcc
     4021 gagcccggcg tcaccggctc gcggtcggga tggccggcaa ggaacgacag gccgccgaag
     4081 gcgttgccga tgcgcccgaa accggggcgg gcggcgtacg gcccggtctg gccgtagccg
     4141 gtgatgcgca ccatcaccag ccccgcattg tgcgcgcgca agtcctcata gccgagcccc
     4201 cagcgctcga gcgtgccggc gcggaagttc tcgcacacca cgtcggcctg ggccgccagc
     4261 cgcttgaaca gcgccgcgcc gtcggcggtc ctcaagtcga gggtgatcga cttcttgttg
     4321 cgggcctcgt tcagccacac cagcgagtcg ccgcaaggag tggcgctgcc gaagtgccgc
     4381 gccgggtcgc cggtgccggg cagctcgacc ttgatcacct cggcgccgaa ctcgcccagc
     4441 agcgtcgcgc agaacggcgc ggcgatgaag gtggcggcgt cgatcacacg gatgccgtcg
     4501 agcgggcgcg gcttggctcc ggcgtctgcc accacgggta cgaggtgtgc gggctactcg
     4561 gccggcgcgc ggctggggag cggagcgggg gcggcttgtc cctcgcccgc aggcaggcgc
     4621 agcgcggcgg tgcctgcggc cagcgccagc accgtcatcg ccaggaacag caagtcgagc
     4681 gagccggtca tgcgatagac gatcgggatc agcgcgaccc cgaacgacga cacgccgagg
     4741 gtgagcatga acttggcgcc gaacacccgc ccgcgccagt gcgccggggt atagcgggcg
     4801 agcagcgcgt tctcggccgg ctggccggcg acgttgaggc cgatcatcag cgccgccacc
     4861 ggcaccagcg ccggatgcac cagcgcgaag gcgaccagca gcaccggcac ctggaggaac
     4921 tgggtggtca ggtagacggt cttgaggcgg aaccggtcgg cgaactcgcc gccgacgatc
     4981 tgggtcaggc tgctggccag gtagaccagg gtcaccatgc cggcgacgcc gaccacgccg
     5041 tcgccgaaca gggcgccgag acgctcggcg aagatcttgg gcagcgcaaa cgaggtcatc
     5101 tggtagatca tgcccgtgca cagcacggtc accgcgagca cggtgaacac gcgccgcgcg
     5161 ccctccgccg gccgctcgcc cgagatccgc gaggtcgccg tggacgtcgc ctcgaacagc
     5221 ccgcgccggc gggcgaacac gaacacgatg ccggccgaca tgcacaccgc gccgggcacg
     5281 aagaacgccc agcgccagct gagatagctc gccaaggtgc ccgccaccag cgccgccgcc
     5341 gcggtcccca gcgagccgaa cacgccgttg atgccgagcg cgcgcccgcg gttgggggcg
     5401 tggcggacca gccacgggat gccgaccggg tggtagatcg aggcgaacag ccccatcagc
     5461 gcgaggccgc ccgacaagct cgccggcccg tcggcgaagc cggtcaggat cgtcgccgcg
     5521 ccgaggccga agaaatagac cgtcatcatg ccggtctcgg accagcggtc gccgagccat
     5581 cccgccggca gcgcgccggc gccgaacagg atgctcatcg ggataccgag cgcgaacagg
     5641 ctggcgtagt ccatgcccca ctcgcgctcg agcaccagca ccacggtcgc gaacagcagc
     5701 acgaacaggt gcgcgaacga atgcgcgacg cccgcgaagc cctgggtgac caggcgcgag
     5761 gtccggctca tttcccgggc tcccggcccg tcctgctcat ccgccccaac gtcatgcggc
     5821 tcacccgtac accaggcgcg gcagccaggt gatcagcggc gggtacatca tgcacgccgc
     5881 caagccggtg gcctggatgg cgaggaacgg cagcgccgcc ttgaagatgt cggccatcgg
     5941 aatatccggc ggcgcgacgc cgcgcaagta gaacaaggcg tagccgaacg gcggcgacag
     6001 gaacgacatc tgcatgttga ccagatagag caccccgaac cacagcggat cgaaccccag
     6061 gcccttgatg atcggaacga atatgggcac gcacagcagc aaaatcccga cccagtcgag
     6121 gaacatgccg agcacgatca ggatcagctg catcaggatc aggatgcccc agcgcccgag
     6181 cccggtcgcc tcgagcgcgc cggtgacgaa ctcctggccg ccctgaagca cgtacaggcc
     6241 gacgaacacg gtggcgccga acatgatcca cagcaccatc gaggtcgcct tcagcgtggt
     6301 gatgcacgcc tcgcgcacgg tcagccagtc gagccggcgg tgcagcgcgg cgaccacgaa
     6361 cgagccgaag gtgccgaggc cggcggcctc gaccggggtc gcgatgccgg tgaagatcac
     6421 gcccagcacc aggacgatca gcaggatcgg cgcggtcatg gtgccgacca gcctgagctt
     6481 gtcgcgcgtc ccgatccgct cggcgagcgg cagggccggg cccatctcgg ggctcaccca
     6541 gcagcgcagc gagacatagg cgatgtacat gcccgacagc atcaggccgg ggatcaccga
     6601 gccgatgaac agctcgccga ccgactgctc ggcgaccacc gcgtagatga tcgccaggat
     6661 gctcggcggg atcaggatgc ccagcgtgcc cccggccatg atcgcgccca tggcgatctt
     6721 gggcgcgtag ttgcgcttga gcatcgccgg cagcgcgatg atgcccatgg tcaccaccgc
     6781 ggcgccgatg atgccgacca tcgcggcgag gatggtcgag gcgacgatgg tcgcgctcgc
     6841 caggccgccg cggatgccgc ccagcagttt gtagatgacg tcgaacatct cgccgatcag
     6901 cccggcgcgt tcgagcatcg ccgccatgaa gatgaacagc gggatcgccg atagctggta
     6961 gtcggtcatc agcgggaaga tgcgcgacgg caccatgttc agcatctgcg tgtcgttgag
     7021 cacgaacagg aacacgcagg ccagcccgcc ggtgacgaag gcgagcggca ggcccgccat
     7081 cagcagcagc agcaggctgc cgaacatcag cagggtcagc catccgatcg ggatctcgac
     7141 acccaccgct caggcctccc gcgcgaccag gatggcgagg tcgcgcagca tcttggccag
     7201 cccctgaatg aggatcagcc ccgcgccgac cgcgatggtg atcttcaccg gccagtactg
     7261 gatcgcccac tcggtgaacg acacctccca gacgccgacc gaatccatca ggaacaccca
     7321 gccggtcgcc agaagcgtgc cggcgaagat gaagaagaac accgaggtca gaaggtcggt
     7381 cgccgcccgc ccgcgccgcg acaggcgggc atagaagatg tccacccgga cgtggctgtc
     7441 ggtcaggtag gcataggcgc ccgcgatcag gtactgcatg ccgaacatca ggaacatgct
     7501 ctcatgcacc cagttggtcg gcgagttgaa caggtagcgc gccaccacct cgtagtagta
     7561 ggcgaacacc gcgatcaccg accaataggc gacgtactcg ccggtccagc ggctgagcgt
     7621 ctcgaaatgg cgaacgatcg aggcgggccg cgccggggcc acgggcgggg ccaccggctc
     7681 gggaacgggg atcgcgggct cgcgctggtt gcacaggcgc gccagcgccg gcaggcggat
     7741 cgcgtcgaac agcagcaaga tgccgattac gatcgccacc gtgcgcgcga tcccggccca
     7801 ctggtcgaac gtcgctgccg ccgcgccctt cgcggcctcg gcacgcgtca gctcggcgcg
     7861 gccggcctcg agcttggcgc cggcgtcttc atcgccggca tcgaccgcgg cccgggcccg
     7921 gtcgacgtcg aactgggcga tcatcacctg cgactcggcc gccgagatca cctcgcgcga
     7981 ggcgcgcgac tcgatattgg cgacgacgat cgcgaccacg aacgggaggt agatcacgcc
     8041 ccgccagttc ttgacgtaga agcggtggat gccggcgaag ccggcggtga tcagcagcag
     8101 gtagccgacc gcgagcgtca ccgccggccg cgggcccgcc aggcgctggc gccggaccag
     8161 cgccatcgcc agcagtggga aggcggcgag ccccgaccag tacagccagt gcggcaggac
     8221 gaaggtgagg tcgggcatga cgggccttga ggcgctggcg gtgtccgggc gagcggccgg
     8281 ggcggcgctc cggccttagc ggccggacgg cgcggcgccg gcggccgcct cggtcagctc
     8341 cacttgagct cttggccctt gatcatgtcc ggagtgacgt agccgaggct gcccgacatc
     8401 atgtagtcga gctggatctt gaacacccgc gcggcgtcct ggtccttgtt cgcccactcg
     8461 aaccagcgcg ggatggcgag gcgggtgaac ttctcgacgt cggcctcggt caggcgcgtg
     8521 accaccgtgc cggcctcggc gaacttggcc cacgactcct ggtcggcctt ctggatcgcc
     8581 gcgtggtgca tgtccgagta cacgtgcacc tccatctcga cgaactgcct catctgcggc
     8641 gacagcgcgt tccaggcccg gttgctgacc gtcaggtcca tcaggtcgac cggctggtac
     8701 agcgacatga agccgggcgg gcccatcgag atgaacttgg tgacctgatg gaagccgagc
     8761 gcgtaattga ccgccgggcc gacataatcc gcgacgtcga tggtgccctt ctcgagtgcc
     8821 gggaagatct ccgagcccgg cagcagcgtg gtcttggcgc cggccgcctg gaacacctcg
     8881 gcgaccatgc cgccgggcag gcgcatcttg cggccgcgga agtcctcgat cgagcggatc
     8941 ggaaccttcg agtggatgat gttggggccg tgctggatcg gcccgacata gtacatgtcc
     9001 tgcttggcga acagctcgcg cgcaatctcg aggccgccga ggccgtagta gaacacgtcc
     9061 cactcgtgcg ggtagcgcgg cccgagcgga taggaggtca ggaacaccga tgccggcatc
     9121 cgccccgccc agtagatggt gaacgggttc atggcgtcga gcacgccgtt cttgaccgcg
     9181 tcgaacagct ggaagtcgcc gaccacgtcc ttggcgccga acggctggaa ggcgagctcg
     9241 ccgccggtct tctcgacaat ggtgctgcac cagctcctga agatgtcgag gccgatgccc
     9301 cccggccacg aggtctggat cttccaggtc gcggtctggg ccctggccgt gccgatgaac
     9361 ggtgccccca ggcggtcggc tgcggcgaac gcggccgcgg cgccggcggc accgaccgcg
     9421 cctttcaaaa ccgcccggcg gctcgcgccg cgagcgcgcg ccgcgggccg cttgtcgcgc
     9481 ccggtggtgg tggattcggt catgttctgg ctctccctgt cgcgtttcgg cggctgcgcc
     9541 ccttcccggg acgtagtcta cacgactccc aggccccgcc cgcaagcggc cttgcaacct
     9601 ccggtcaggc gccggggccg gcgccgttga gcaccgcggg atcgcgcacc gcgccgaacg
     9661 aggcgctgcg caccatcgcg ccgtaggccc tgagcgcgcg cgagacgatc cgcgcccgat
     9721 tgaccggctt ccagccgccg tcggcgcgcg ccgccatcgc cgtccggcgg cgggcgaggg
     9781 cggcgtcgtc gaccgctagg cggatgctgc gcgccgggat gtcgaaggcg atctcgtcgc
     9841 cgtcctcgat cagcgccagc tcgcccccgt ccgcggcttc gggcgagaca tggccgaccg
     9901 acaggcccga ggtcgcgccc gagaagcggc cgtcggtg
//
LOCUS       DASWHU010000338         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_207273, whole genome shotgun sequence.
ACCESSION   DASWHU010000338 DASWHU010000000
VERSION     DASWHU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207273"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGB88_12680"
     CDS             <1..592
                     /locus_tag="VGB88_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9648352.1"
                     /translation="VALDSLAVPAGLDFAGYDTAAAGSPALWDDYAPGERIDHIDGTT
                     VEAAEHMAATRLYQNTARAHFDRRRAAATRFGRRIVYGGHVISLARALSFNGLANAQW
                     VAAINGGRHVAPVFAGDTVYAWSEVLEKAPLAGRDDVGALRLRTVAAKDRDCADFPDR
                     DAAGRPDASVVLDFDYTVLMPRRIVRTPVDLLSGRV"
     gene            643..1038
                     /gene="sdhC"
                     /locus_tag="VGB88_12685"
     CDS             643..1038
                     /gene="sdhC"
                     /locus_tag="VGB88_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX9648353.1"
                     /translation="MAQADRPLSPHLQIYRPQLTSVLSILHRITGVVLGLGTALLVWW
                     LAAAASGPEAFAAVQAFNASILGRLLLLGWTFALFYHLCNGVRHLAWDAGWGFELGAV
                     YRSGWAVVAGSGALTVIAWIAGYAVRGAP"
     gene            1044..1418
                     /gene="sdhD"
                     /locus_tag="VGB88_12690"
     CDS             1044..1418
                     /gene="sdhD"
                     /locus_tag="VGB88_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX9648354.1"
                     /translation="MRAPLAEARGLGAAKDGVGHWWAQRVSALALVPLSLWLVASLVA
                     MTGAGHAEVSAWLARPWVAVTLVLTLFATFFHLKLGLQVVVEDYVEGRVARTALELLV
                     TFAAIALGTGAVFAVLKVALGG"
     gene            1425..3215
                     /gene="sdhA"
                     /locus_tag="VGB88_12695"
     CDS             1425..3215
                     /gene="sdhA"
                     /locus_tag="VGB88_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX9648355.1"
                     /translation="MSDAYPVTDHAYDVVVIGAGGAGLRATFGMAEKGLKTGCLTKVF
                     PTRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYMCREAAAAVVE
                     LEHFGVPFSRTPEGKIYQRAFGGMTTRFGEGTAQRTCAAADRTGHAILHTLYQQALRA
                     RAEFMVEYVAIDLIMDDDGACRGVMAWNLDDGTLHRFRAHIVVLATGGYGRTYFSCTS
                     AHTCTGDGNAMVLRAGLACQDMEFIQFHPTGIYGAGCLITEGVRGEGGYLTNSEGEHF
                     MERYAPHAKDLASRDVVSRSMTIELREGRGVGPHKDYIYLHLEHLDPDIIAERLPGIA
                     ETARIFAGVDVTKEPIPVLPTCHYNMGGIPTNHHGEVVTLRDGDPEAVVPGLMAIGEA
                     ACVSVHGANRLGSNSLLDIVVFGRAAALRAAELVRPGEPHKDLAAGAGDEAVARLDRL
                     RHAKGGTPTAAMRLEMQRTMQDHCAVFRTGEVLAEGARKLEALWRRLGDIRVSDRSMI
                     WNSDLVETLEYENLLAQAVAAIAGAANRTESRGAHAREDYPGRDDEAWLKHSLIWVDE
                     AGRARIDYRPVHLNTLTNEVQPIPPKPRVY"
     gene            3259..4038
                     /locus_tag="VGB88_12700"
     CDS             3259..4038
                     /locus_tag="VGB88_12700"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX9648356.1"
                     /translation="MADFTLPANSRVRAGTTHRAPAGATNVRTFATYRWSPDDSANPR
                     LDRFEIDLDGCGPMVLDALIKIKNETDSTLTFRRSCREGVCGSCAMNIDGTNTLACTK
                     YISEVTGEVRIYPLPHLPVVKDLVADLSHVAAQYASIKPWLRAESPPPPDRERLQSPE
                     ERRRLDGLYECILCFCCTTACPSYWWTGERYLGPAALLQAYRWLADSRDEAAGERLDE
                     LEDPFKLYRCHTIMNCTRTCPKGLNPAEAIGAIKRLMVARR"
     gene            4294..5256
                     /locus_tag="VGB88_12705"
     CDS             4294..5256
                     /locus_tag="VGB88_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX9648357.1"
                     /translation="MMYLLAAGGLMLLLGGGELLVRGAVGLAQSFGVAPLVIGLTVVA
                     FGTSAPELVVCLEAAFGDHPDIVLGNIVGSNIANLLAIIGIAALLRPIAIASGVVRRE
                     GTILLAVSAAFLAVCLTGELTRAVGGVMVLLLAAYTWWSFWAARRRSRAEAREFKRAT
                     EEQAPPSARLALVFTALGIAGVIAGADLLIEGAVGIAQAAGLSEAVIGLTLVALGTSL
                     PELATSGMAAVRGHSDLAVGNAVGSNLFNMLAIGGITALAVPVMVPAEVIGFDLWVML
                     AVTLALVALMAVNGRIGRSHGAALTASYAGYVALLVTGAAPRLM"
     gene            5319..6431
                     /locus_tag="VGB88_12710"
     CDS             5319..6431
                     /locus_tag="VGB88_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9648358.1"
                     /translation="MRATVAIVMLIVGLVIGGLIGRFALAPARVDTSIETGGTPGEPA
                     PAAAAPVRWKLASAYAHSTAVLGETVDHFIDSLGAMSQGDIELRFYEPGALIPALEVF
                     DAVSSGSVESGYSTPGFWAGKEPALQLFASVPFGPSAPEYMAWYYMGGGQAMLDELYA
                     KHDIKPLLCGLFPPEASGWFRNEITGPDDLKGLKMRFFALGARVMEKLGVSTQLLAGG
                     DIFPALELGTIDATEYASPAIDRNLGFYQVAKHYYFPGWHQQSTWVEIIVNLDKWNGL
                     SESQRSMFEVACGDMVRYSIALGEAAQVPAMEFLQQQGVVFHKWSPEMLGLFKSGWDQ
                     VLAEEVAKDPTFARVWASLNDFRTRYATWRDLGYLN"
     gene            6473..>7920
                     /locus_tag="VGB88_12715"
     CDS             6473..>7920
                     /locus_tag="VGB88_12715"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9648359.1"
                     /translation="MTDTLHHFIAGRKVAGSSGRKGEVYNPATGEVAKTVPFAGAADV
                     DQAVAAARAAFPRWAATTPIARARVMFRLKELIERNLDELAVAVSSEHGKTIPDAKGS
                     ITRGLEVVEFACGIPHLLKGEFTEAVGTGVDSYSMRQPIGVCAGITPFNFPAMIPLWM
                     APLAIACGNSFILKPSERDPSCPLRLAELFTEAGVPDGVFNVVNGDKEAVDAILAHPD
                     IGAVSFVGSTPIAEHVYATGSAHGKRVQALGGAKNHMVVMPDADLDQAVDALMGAAYG
                     SAGERCMAVSVAVAVGGTGEPLIERLVPRIAGLKIGPYTDPDAEMGPIVTRQALTRIR
                     ALVESGVKQGAELVIDGRDYALQGYENGFFMGGCLFDRVTPEMDIYKQEIFGPVLSVV
                     RAPDYDSALGLVCDSPYGNGVAIFTRDGDCAKDFAQRANIGMVGVNVPIPVPVAYHSF
                     GGWKRSKFGAANTHGMEGVRFYTQIKTVTARWP"
BASE COUNT         1110 a         2856 c         2774 g         1180 t
ORIGIN      
        1 cgtcgcgctc gactcgctcg cggtgccggc gggcctcgac ttcgccggct acgataccgc
       61 cgccgccggg tcgcccgcgc tgtgggacga ctacgcgccc ggcgagcgca tcgaccacat
      121 cgacggcacg acggtggagg ccgccgagca catggcggcg acgcggctct accagaacac
      181 agcgcgcgcc catttcgacc gccggcgcgc ggcggcgacc cgattcggcc ggcgcatcgt
      241 ctatggcggc cacgtcatca gcctggcgcg ggcgctgagc ttcaacggcc tcgccaacgc
      301 ccagtgggtg gcggcgatca acggcggccg gcacgtggcg ccggtgttcg cgggcgacac
      361 ggtctacgct tggtcggagg tgctggagaa ggcgccgctc gccggccgcg acgatgtcgg
      421 cgcgctgcgc ctgcgcacgg tcgcggccaa ggaccgcgac tgcgccgatt tccctgaccg
      481 cgatgccgca gggcgccccg acgcctcggt cgtgctcgat ttcgactaca cggtgctgat
      541 gccccgccgc atcgtccgaa ccccggttga cttgctttcg ggcagggtct agttcgtagg
      601 cggtcgcggc tcccgcgacc gtcctcacct gggagcttcc ggatggcgca ggcggaccgg
      661 ccgctgtcgc cgcatctcca gatttatcgc ccgcagctga cctcggtgct gtcgatcctg
      721 caccggatca ccggcgtcgt gctgggcctg ggcacggccc tgctggtgtg gtggctggcg
      781 gccgcggcga gcgggcccga ggcgttcgcc gcggtccagg cgttcaacgc ctcgatcctc
      841 ggccggctgc tgctgctggg ctggaccttc gcgctgttct accacctgtg caacggcgtc
      901 cgtcacctgg cctgggacgc gggctggggg ttcgagctcg gcgccgtcta ccgctcgggc
      961 tgggcggtgg tcgcgggcag cggggcgctg accgtgatcg cctggatcgc cggctacgcc
     1021 gtgcggggcg cgccgtgacc ggcatgcgcg cgccgctcgc cgaggcgcgc ggcctcggcg
     1081 cggccaagga cggcgtcggc cactggtggg cgcagcgggt gagcgcgctg gcgctggtgc
     1141 cgctgagcct gtggctggtg gcctcgctgg tggcgatgac cggcgccggc cacgccgagg
     1201 tgagcgcctg gctcgcccgg ccgtgggtcg cggtcaccct cgtgctgacc ctgttcgcga
     1261 ccttctttca cctcaagctc ggcctgcagg tggtggtcga ggactacgtc gagggccggg
     1321 tcgcgcgcac cgcgctcgag cttctggtca ccttcgccgc catcgcgctc ggcaccggcg
     1381 cggtgttcgc cgtgctcaag gtcgcgcttg gcggctgagc ggcgatgagc gacgcctatc
     1441 cggtcaccga ccatgcttac gacgtggtcg tcatcggcgc cggcggtgcc ggcctgcgcg
     1501 ccaccttcgg catggccgag aaggggctca agaccggctg cctgaccaag gtgttcccga
     1561 cccgcagcca caccgtcgcc gcccagggcg gcatctcggc ggcgctcggc aacatgggcg
     1621 aggacgactg gcgctggcac atgtacgaca cggtcaaggg ctcggactgg ctcggcgacc
     1681 aggacgcgat cgagtacatg tgccgcgagg ccgcggcggc ggtggtcgag ctcgagcatt
     1741 tcggggtgcc gttctcgcgc acgcctgagg gcaagatcta ccagcgcgcc tttggcggca
     1801 tgaccacccg tttcggcgag ggcaccgccc agcgcacctg cgctgccgcc gaccgcaccg
     1861 gccacgccat cctgcacacc ctctaccagc aggcgctgcg cgcccgggcc gagttcatgg
     1921 tcgagtacgt cgccatcgac ctgatcatgg acgacgacgg cgcctgccgc ggggtgatgg
     1981 cgtggaacct cgacgacggc accctccacc gcttccgcgc ccacatcgtg gtgctggcca
     2041 ccggcggcta cggccgcacc tacttctcgt gcacctcggc gcacacctgc accggcgacg
     2101 gcaacgccat ggtgctgcgc gccggcctcg cctgccagga catggagttc atccagttcc
     2161 acccgaccgg catctacggc gccggctgcc tgatcaccga gggcgtgcgc ggcgagggcg
     2221 gctacctgac caactcggag ggcgagcact tcatggagcg ctacgcgccc cacgccaagg
     2281 acctcgcctc gcgcgacgtg gtcagccggt cgatgaccat cgagctgcgc gagggccgcg
     2341 gcgtcggccc gcacaaggac tacatctacc tgcatctcga gcacctggac cccgacatca
     2401 tcgccgagcg cctgcccggc atcgccgaga ccgcgcgcat cttcgccggc gtcgacgtga
     2461 ccaaggagcc gatcccggtg ctgccgacct gccactacaa catgggcggc atcccgacca
     2521 accatcacgg cgaggtggtg acgctccgcg acggcgaccc cgaggcggtg gtgccggggc
     2581 tgatggcgat cggcgaggcg gcgtgcgtgt cggtgcacgg cgccaaccgg ctcggctcca
     2641 actcgctgct cgacatcgtc gtgttcggcc gcgccgcggc cctgcgcgcg gccgagctcg
     2701 tgcgcccggg cgagccgcac aaggaccttg ccgccggcgc cggcgacgag gcggtcgcgc
     2761 gcctcgaccg gctccgccac gccaagggcg gcaccccgac cgcggccatg cgccttgaga
     2821 tgcagcgcac catgcaggac cattgcgcgg tgttccgcac cggcgaggtg ctggccgagg
     2881 gcgcgcgcaa gctcgaggcg ctgtggcggc ggctcggcga catccgggtc tctgaccgct
     2941 ccatgatctg gaactcggac ctggtcgaga cgctcgagta cgagaacctg ctcgcccagg
     3001 cggtggcggc gatcgcgggc gccgccaacc gcaccgagag ccgcggcgcg cacgcccgcg
     3061 aggactaccc ggggcgcgac gacgaggcct ggctcaagca cagcctgatc tgggtcgacg
     3121 aagccgggcg ggcgcgcatc gactaccgcc ccgtccacct caacacgctg accaacgagg
     3181 tccagccgat cccgcccaag ccgcgggtct attgagcgca cggcgaaccg cgccgcccga
     3241 cgatcgaggc accgaccgat ggccgacttc acccttcccg cgaactcgcg agtgcgcgcg
     3301 ggcaccacgc accgggcgcc ggcgggcgcg accaacgtca ggaccttcgc gacctaccgc
     3361 tggagcccgg acgacagcgc caacccgcgc ctcgaccgct tcgagatcga cctggacggg
     3421 tgcgggccga tggtcctcga cgccctcatc aagatcaaga acgagaccga ctcgacgctc
     3481 accttccgcc gctcgtgccg ggagggcgta tgcggcagct gcgccatgaa catcgacggc
     3541 accaacaccc tcgcctgcac caagtacatc agcgaggtca cgggcgaggt gcgcatctac
     3601 ccgctgccgc atttgccggt ggtcaaggac ctggtggcgg acttgagcca cgtcgccgcg
     3661 caatacgcct cgatcaagcc gtggctgcgc gccgagtcgc cgccgccgcc cgaccgcgag
     3721 cgcctgcaat cgcctgagga gcgccgccgg ctcgacggcc tctacgagtg catcctgtgc
     3781 ttctgctgca ccacggcctg cccgagctac tggtggaccg gcgagcgcta tctcggcccg
     3841 gcggcgctgc tgcaggccta ccggtggctc gccgacagcc gcgacgaggc ggcgggcgag
     3901 cggctcgatg agctcgagga cccgttcaag ctctatcgct gccacacgat catgaactgc
     3961 acccggacct gccccaaggg gctcaacccg gccgaggcga tcggcgcgat caagcgcctg
     4021 atggtcgcac gccgctagag cggtatgata tttggtcgaa ccgccggatt ttattggtcg
     4081 tcaccccgga aacggcgaag ccgttatccg gggtccatac gcctttgcaa ccaatcggcc
     4141 gctcgtgcaa gcggcgagat ggaccccggc tcgcggcgct tcgcgccttg gccggggtga
     4201 cgggtcaagg atgatcaggt ttcaaatcat cacgctctag ccaccgcccg cggccgcgga
     4261 cgcgccgcgt cccgcccaca gggagctgac cggatgatgt acctgttggc cgccggcggg
     4321 ctgatgctcc tgctcggcgg cggcgagctg ctggtccgcg gcgcggtcgg cctggcgcag
     4381 agcttcggcg tcgcgccgct ggtgatcggg ctgacggtgg tcgcattcgg cacctcggcg
     4441 cccgagctgg tggtgtgcct cgaggccgcg ttcggcgatc accccgacat cgtgctcggc
     4501 aacatcgtcg gctccaacat cgccaacctg ctcgccatca tcggcatcgc cgcgctgctg
     4561 cgcccgatcg ccatcgcctc gggcgtggtc cggcgcgagg gcaccatcct gctcgcggtc
     4621 tcggccgcgt tcctcgccgt ctgcctgacc ggcgagctga cccgcgccgt cggcggggtc
     4681 atggtgctgc tgctcgccgc ctacacctgg tggtcgttct gggccgcccg gcggcgcagc
     4741 cgcgccgagg ccagggagtt caagcgcgcg accgaagaac aggcgccgcc ctcggcccgg
     4801 ctcgccctgg tgttcaccgc gctcggcatc gccggcgtga tcgcgggcgc cgacctgttg
     4861 atcgagggcg cggtcggcat cgcgcaggcg gcggggctgt cggaggcggt gatcgggctc
     4921 accctggtgg cgctgggcac gtcgctgccg gagcttgcga cctcgggcat ggcggcggtg
     4981 cgcggccaca gcgaccttgc ggtcggcaac gccgtcggca gcaacctgtt caacatgctc
     5041 gccatcggcg gcatcaccgc gctggcggtg ccggtgatgg tgccggcgga ggtgatcggc
     5101 ttcgacctgt gggtgatgct tgcggtcacg ctcgcgctcg tggcactgat ggccgtcaac
     5161 ggccgcatcg gccgcagcca tggcgcggcg ctgacggcga gctatgcggg ctatgtcgcg
     5221 ctgctggtca ccggcgccgc gccgcggctg atgtgaccgc gggctgtcgc gccgccgcat
     5281 tttgcgtatg ctagccggcg caacgagggg aggacaccat gcgtgcaacc gtcgcgatcg
     5341 tgatgctgat cgtcggcctg gtgatcggcg ggctgatcgg ccgcttcgcg ctcgccccgg
     5401 cccgggtcga cacctcgatc gagaccggcg ggacgccggg cgagcccgcg cccgctgccg
     5461 ccgcgccggt gcgctggaag ctggccagcg cctacgccca ctcgaccgcg gtgctcggcg
     5521 agaccgtcga ccacttcatc gacagcctgg gggcgatgtc gcaaggcgac atcgagctca
     5581 ggttctacga gccgggggcg ctgatcccgg cgctcgaggt gttcgacgcg gtctcctccg
     5641 gctcggtcga gtcgggctac tcgacgcccg gcttctgggc cggcaaggag ccggcgctgc
     5701 agctgttcgc gtcggtcccc ttcggtccca gcgcgcccga gtacatggcc tggtactaca
     5761 tgggcggcgg ccaggcgatg ctcgacgagc tgtacgccaa gcacgacatc aagccgctcc
     5821 tgtgcgggct gttcccgccc gaggcgtcgg gctggttccg caacgagatc accggccccg
     5881 acgacctcaa gggcctgaag atgcgcttct tcgcgctcgg cgcgcgggtg atggagaagc
     5941 tcggcgtctc cacccagcta ttggccggtg gcgacatctt cccggcgctc gagctcggca
     6001 cgatcgacgc caccgaatat gccagccccg cgatcgaccg caatctcggc ttctaccagg
     6061 tcgccaagca ctactacttc ccgggctggc accagcagtc gacctgggtc gagatcatcg
     6121 tcaacctgga caagtggaac gggctcagcg agtcccaacg cagcatgttc gaggtcgcgt
     6181 gcggcgacat ggtccgctat tcgatcgcgc tcggcgaggc cgcgcaggtg ccggcgatgg
     6241 agttcctcca gcagcaggga gtggtgttcc acaagtggtc gcccgagatg ctggggctgt
     6301 tcaagtcggg ctgggaccag gtgctcgccg aggaggtggc gaaggacccg accttcgccc
     6361 gcgtctgggc ctcgctcaac gatttccgca cgcgctatgc gacctggcgc gacctgggtt
     6421 acctgaactg agcggcgcgg cgcgccggct cggacgagga ttgggaggcg acatgaccga
     6481 cacgcttcat cacttcatcg ccggcaggaa ggtggccggc agcagcggcc gcaagggcga
     6541 ggtctacaac ccggccaccg gcgaggtcgc caagaccgtg ccgttcgcgg gcgcggccga
     6601 cgtcgaccag gcggtggccg cggccagggc ggcgttcccg cgctgggcgg cgaccacccc
     6661 gatcgcgcgc gcgcgggtga tgttccggct caaggagctg atcgagcgca acctcgacga
     6721 gctcgcggtg gcggtcagct ccgagcacgg caagaccatc cccgacgcca agggctcgat
     6781 cacccgcggc ctcgaggtgg tcgagttcgc ctgcggcatc ccgcacctgt tgaagggcga
     6841 gttcaccgag gcggtcggca ccggcgtcga cagctactcg atgcgccagc cgatcggggt
     6901 gtgcgccggc atcaccccgt tcaacttccc ggccatgatc ccgctgtgga tggcgccgct
     6961 cgccatcgcc tgcggcaaca gcttcatcct caagccgtcc gagcgcgacc cctcctgccc
     7021 gctgcggctc gccgagctgt tcacggaggc gggggtcccc gacggcgtgt tcaacgtcgt
     7081 caacggcgac aaggaggcgg tggacgcgat cctcgcccat cccgacatcg gcgcggtgag
     7141 cttcgtcggc tcgaccccga tcgcggagca cgtctacgcc accggctcgg cccacggcaa
     7201 gcgtgtccag gcgcttggcg gcgccaagaa ccacatggtg gtgatgcctg acgccgacct
     7261 cgaccaggcg gtcgatgcgc tgatgggcgc ggcctacggc tcggccggcg agcgctgcat
     7321 ggcggtctcg gtcgcggtcg cggtcggcgg caccggcgag ccattgatcg agcggctggt
     7381 cccgcgcatc gccggcctca agatcggccc ttacaccgat cccgacgccg agatgggccc
     7441 gatcgtcacc cgccaggcgc tgacccgcat ccgcgccctc gtcgaatcgg gcgtcaagca
     7501 gggcgcggag ctggtcatcg acggccgcga ttacgcgctc cagggctacg agaacggctt
     7561 cttcatgggc ggctgcctgt tcgaccgcgt cacgcccgag atggacatct acaagcagga
     7621 gatcttcggc ccggtgctgt cggtggtgcg cgcgcccgac tacgacagcg cgctcgggct
     7681 ggtgtgcgac agcccctacg gcaacggggt cgcgatcttc acccgcgacg gcgactgcgc
     7741 caaggacttc gcccagcgcg ccaatatcgg catggtcgga gtcaacgtgc cgatcccggt
     7801 gccggtcgcc tatcacagct tcggcggctg gaagcgctcg aagttcggcg ccgccaacac
     7861 ccacggcatg gagggggtgc gcttctacac ccagatcaag acggtcaccg cgcgctggcc
//
LOCUS       DASWHU010000339         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_951517, whole genome shotgun sequence.
ACCESSION   DASWHU010000339 DASWHU010000000
VERSION     DASWHU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951517"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VGB88_12720"
     CDS             complement(<1..451)
                     /locus_tag="VGB88_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9648360.1"
                     /translation="MAAVGRVAIRPLARGDLAAVVALDAEATGMPKAAYWERLFDAYA
                     PAGANRHALAAELDGALVGYVLGEVRSWEFGSQPCGWVFAINVARRLQEAGIGTRLLE
                     ALCAAFRTDGVATVRTMVARDATLLLSFFRAQGMTAGPFLELEMPLAD"
     gene            524..2218
                     /gene="boxC"
                     /locus_tag="VGB88_12725"
     CDS             524..2218
                     /gene="boxC"
                     /locus_tag="VGB88_12725"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX9648361.1"
                     /translation="MAESTPAAGPGHGAGANLPAEVDFRTAPERYRHWRLAIDGPVAT
                     LALDVDEAAGLRPGYELKLNSYDLGVDIELADAVQRLRFEHPGVRAVVITSAKARLFS
                     AGANIRMLATASHAEKVNFCKFTNETRCAIEQAGAESGQHYVAALNGTASGGGYELAL
                     ACERIVLADDGASAVSLPELPLLAVLPGTGGLTRLVDKRKVRRDRADVLCTTAEGVRG
                     RRALDWGLVDALARPSELADAAHALALALADEQDRPSAADAIALGPLAREASPDRLSY
                     DGLEVDIDGDRRLATVTVKGPAAPAPADAAGIRAAGDAFWPLALARALDDAVLHLRTN
                     RADIGTWVLRSEGDGEMVAAYDALLAAQAGDRLAREVTLYWRRVLKRLDLSSRSLIAL
                     IEPGSCFAGMLLELALAADRSYMRDAEDEAPALRLTAMNFGPLAAANGLSRLATRFLG
                     APAALEAARAAADRPLGAAEAEAIGLVSFTPDDIDWDDEVRLALEERASFSPDALTGL
                     EANLRFAGPETLETKIFGRLSAWQNWIFQRPNATGAGGALTVYGSGRRSDFDLRRV"
     gene            2221..>2543
                     /locus_tag="VGB88_12730"
     CDS             2221..>2543
                     /locus_tag="VGB88_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX9648362.1"
                     /translation="MAIDYGERIPNNVELAGNRRLQRALESWQPRFIDWWRELGPAGF
                     QAAEVYLRTAVSVDAGGWAHFGHVKMPDYRWGIFLAEPEPDRRIAFGDHKGEPAWAEV
                     PGEYRA"
BASE COUNT          343 a          965 c          904 g          331 t
ORIGIN      
        1 cgtcggcgag cggcatctcg agctcgagga acggcccggc ggtcatgccc tgggcgcgga
       61 agaacgacag cagcaaagtg gcatcgcgcg cgaccatggt ccgcaccgtg gccacgccgt
      121 cggtgcggaa ggcggcgcac agcgcctcga gcaggcgcgt gccgatgccc gcctcctgca
      181 accggcgcgc gacgttgatc gcgaacaccc agccgcacgg ctgcgagccg aactcccagc
      241 ttcgcacctc gcccaggacg tagccgacca gcgccccgtc cagctcggcg gcgagggcgt
      301 ggcgattcgc cccggccggc gcgtaggcgt cgaacagccg ctcccagtag gcggccttgg
      361 gcatgccggt ggcctcggca tcgagcgcca ccaccgccgc gaggtcgccc cgcgcgagcg
      421 ggcgaatcgc caccctgccc accgccgcca ttcgccgctt gactccgctt ggcccccggc
      481 taccgttgca attcagtata gcaataccga attggatgcg gccatggccg agtccacgcc
      541 tgcggccggg cccggtcacg gcgccggcgc caacctgccg gccgaggtcg acttccgcac
      601 cgcgcccgag cgctaccgcc actggcggct cgcgatcgac gggccggtgg cgaccttggc
      661 gcttgacgtc gacgaggcgg cgggcctgcg cccgggctac gagctcaagc tcaactccta
      721 tgacctcggc gtagacatcg agctcgcgga cgcggtgcag cggctgcgct tcgagcatcc
      781 cggcgtgcgc gcggtggtga tcacctcggc gaaagcgcgc ctgttctcgg ccggagccaa
      841 catccgcatg ctggcgaccg cgagccacgc cgagaaggtg aacttctgca agttcaccaa
      901 cgaaacccgc tgcgcgatcg agcaggcggg cgccgagtcg ggccagcact acgtcgccgc
      961 gctcaacggc accgcctcgg gcggcggcta cgagcttgcg cttgcctgcg agcgcatcgt
     1021 gctcgccgac gacggcgcga gcgcggtctc gctgcccgag ctgccgctgc tcgcggtgct
     1081 gcccggcacc ggcgggctga cccgcctggt cgacaagcgc aaggtgcgcc gcgaccgcgc
     1141 cgacgtcctg tgcaccacgg ccgagggcgt gcgcggcagg cgcgcgctcg actgggggct
     1201 ggtggatgcg ctggcgcgcc cgtcggagct cgccgacgcc gcgcacgccc tggcgctggc
     1261 gctggctgat gagcaggatc ggccgagcgc cgcggacgcc atcgcgctcg gcccgctcgc
     1321 ccgcgaggcg agcccggatc gcctgtccta tgacgggctc gaggtggaca tcgacggcga
     1381 ccgccgcctc gccaccgtca cggtcaaggg cccggccgcg cccgcgcccg ccgatgccgc
     1441 gggaatcagg gccgcgggcg atgcgttctg gccgctcgcg ctcgcccgcg cgctcgacga
     1501 cgcggtcctc catctgcgca ccaaccgcgc cgacatcggc acctgggtgc tgcgcagcga
     1561 gggcgacggc gagatggtcg ccgcctacga tgcgctgctt gccgcccagg ccggcgaccg
     1621 gctggcgcgc gaggtgacct tgtactggcg gcgggttctc aagcgcctcg acctgtcctc
     1681 gcgcagcctg atcgcgctga tcgagccggg aagctgcttc gccggcatgc tcctcgagct
     1741 cgcgctcgcc gctgaccgga gctacatgcg cgacgccgag gacgaggcgc ccgcgctccg
     1801 cctgacggcg atgaacttcg gcccgctggc tgcggccaac gggctgtcgc gcctcgccac
     1861 ccgcttcctc ggcgcgcccg cggcgctgga ggccgcgcgc gcggccgccg accgcccgct
     1921 cggagccgcc gaggccgagg cgatcgggct ggtgagcttt acgcccgacg acatcgactg
     1981 ggacgacgag gtccgccttg cgctcgagga gcgggcgagc ttctcgcccg acgcgctgac
     2041 cgggctcgag gccaacctgc gcttcgccgg gcccgagacg ctggagacca agatcttcgg
     2101 ccgcttgagc gcctggcaga actggatctt ccagcggccc aatgccaccg gcgccggcgg
     2161 cgcgctcacc gtctacggca gcggccggcg cagcgacttc gacttgcggc gggtgtgacc
     2221 atggcgatcg actacggcga gcggattccc aacaacgtcg agcttgcggg caaccgccgc
     2281 ctgcagcggg cgctcgagtc gtggcagccg cgcttcatcg actggtggcg cgagctcggc
     2341 cccgccggct tccaggccgc cgaggtctat ctccgcaccg cggtcagcgt cgatgccggc
     2401 ggctgggcgc atttcggcca cgtcaagatg cccgactacc gctggggaat cttcctcgcc
     2461 gagcccgagc ccgaccggcg catcgctttc ggcgaccaca agggcgagcc cgcctgggcc
     2521 gaggtgccgg gcgagtatcg ggc
//
LOCUS       DASWHU010000340         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_952492, whole genome shotgun sequence.
ACCESSION   DASWHU010000340 DASWHU010000000
VERSION     DASWHU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952492"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGB88_12735"
     CDS             complement(<1..569)
                     /locus_tag="VGB88_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9648363.1"
                     /translation="MGALVEGKWQSRAPRARTRDGQFVRRDSQFRDWIDAGGETPFAP
                     AAGRYHLYVSLACPWAHRTVIFRALKGLEEVIGLSVVDPYMGSDGWRFSDAPGCIRDT
                     VNGARFLHQIYTLARPGYSGRVTVPVLWDTHSGTIVNNESSEIIRMFNAAFDSLTGNR
                     LDFYPAPLRAEIDAVNAEVYANVNNGVYRA"
     gene            640..1134
                     /locus_tag="VGB88_12740"
     CDS             640..1134
                     /locus_tag="VGB88_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648364.1"
                     /translation="MRSLKILALMAGLLTALPQAGSAAEAISGNALPARANLLMIAGQ
                     VVRLFAIEVLHPEQTCDNTLVVYPCGQVALARLAHAIVRQTVVCVPVAEIHQDGPDDP
                     IVSVCHVGDIDLGYWLVRTGFALADRRHPDVYGDAEAKAREVGNGIWHGPFVMPWDWA
                     AGVR"
     gene            1107..1364
                     /locus_tag="VGB88_12745"
     CDS             1107..1364
                     /locus_tag="VGB88_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648365.1"
                     /translation="MGLGRRRALIAAGLGLACAVAPAAGAGEGEDRDQVIAAAAERGC
                     VAREVKRLGAPHQGVIEVRCADSHVIWFEKVDGAWVLKPLG"
     gene            1375..>1708
                     /locus_tag="VGB88_12750"
     CDS             1375..>1708
                     /locus_tag="VGB88_12750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9648366.1"
                     /translation="MAAAGVIGWAGALGSALWLALLALPWRPWSTAERLEPDRDEGQA
                     ADLSDIAVLIPARDEAQVIGATLDALAAQGTGLDVVVVDDQSLDGTGEVARARAPVRV
                     VAGAGLPAG"
BASE COUNT          218 a          593 c          647 g          250 t
ORIGIN      
        1 gcgcggtaga cgccgttgtt gacgttggcg taaacctcgg cgttgaccgc gtcgatctcg
       61 gccctgagcg gcgccgggta gaagtcgagg cggttgccgg tgagcgaatc gaacgccgcg
      121 ttgaacatgc ggatgatctc ggacgattcg ttgttgacga tcgtgccgct atgcgtgtcc
      181 cacagcaccg gcaccgtcac ccggccgctg tagccgggcc tggcaagggt gtagatctgg
      241 tgcaggaagc gcgcgccgtt gacggtgtcg cggatgcagc cgggcgcgtc gctgaaccgc
      301 cagccgtcgc tgcccatata cgggtcgacc accgacaagc cgatcacctc ctcgagcccc
      361 ttgagcgcgc ggaagatcac ggtgcggtgc gcccacgggc aggcgagcga gacgtagagg
      421 tggtagcgcc cggccgcggg cgcgaacggg gtctcgccgc cggcgtcgat ccagtcccgg
      481 aactgcgagt cgcggcgcac gaactgcccg tcgcgcgtgc gtgcgcgcgg cgcccgcgat
      541 tgccacttgc cctcgaccaa tgcgcccatc cgcgcctccg tccggttcgc ggctcgccgc
      601 gaccttgccg gcgccacggg ttcgacttat aagcagttga tgcgcagtct caaaattctc
      661 gccctgatgg cggggctgct caccgccctt ccgcaggccg gatcggcggc ggaggccatc
      721 tcgggcaacg ccctgccggc gcgggccaac ctgctgatga tcgcgggcca ggtcgtgcgc
      781 ctgttcgcga tcgaggtgct gcatccggag cagacctgcg acaacacgct ggtggtctat
      841 ccgtgcggcc aggtcgcgct ggcgcgcctc gcccacgcga tcgtcaggca gacggtggtg
      901 tgcgtgccgg tggcggagat ccaccaggac gggcccgacg acccgatcgt ctcggtctgc
      961 catgtcggcg acatcgacct cggctactgg ctggtgcgca ccggcttcgc gctcgccgac
     1021 cgcaggcacc cggacgtcta cggcgacgcc gaggccaagg cgcgcgaggt cggcaacggc
     1081 atctggcacg gcccgttcgt gatgccgtgg gactgggccg ccggcgtgcg ctgatcgccg
     1141 ccggcctggg gctcgcctgc gccgtcgccc cggcggccgg cgccggcgag ggcgaggatc
     1201 gcgaccaggt catcgccgcc gccgccgaac gcggctgtgt cgcgcgcgag gtgaagcggc
     1261 tcggcgcgcc gcaccagggc gtcatcgagg tgcgctgcgc cgattcgcac gtcatctggt
     1321 tcgagaaggt cgacggcgcg tgggtgctga agccgctcgg ctgaccgcgg ggcgatggct
     1381 gccgccggcg tgatcggctg ggcgggcgca ctgggcagcg cgctgtggct ggcgctgctc
     1441 gccctgccat ggcggccgtg gagcaccgcc gagcgcctgg agcctgatcg ggacgagggg
     1501 caggccgccg acctgtcgga catcgcggtg ctgatcccgg cgcgcgacga ggcgcaggtg
     1561 atcggcgcga ccctcgatgc gctcgccgcc cagggcaccg gcctcgacgt ggtggtggtc
     1621 gacgaccagt cgctggacgg caccggcgag gtcgcgcgcg cccgcgcgcc ggtgcgggtg
     1681 gtggcgggcg cgggcctgcc cgccggct
//
LOCUS       DASWHU010000341         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2453424, whole genome shotgun sequence.
ACCESSION   DASWHU010000341 DASWHU010000000
VERSION     DASWHU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453424"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VGB88_12755"
     CDS             <1..594
                     /locus_tag="VGB88_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9648367.1"
                     /translation="GPAPLPLFAAAEARHGAALAGPEPAVSLPVAGLGEEVADDYRSL
                     RLSLKAHPLALLRNELGRRRAVPAARLADIRAGARVRVAGLVLVRQRPGTAKGVIFAT
                     LEDETGTANVIVWPDVFERFRRTVLAAKLLCVEGEVQREGIVLHVVAARLEDWTPLLG
                     RLTETRTELSPALARADHVARPGHDPRTLPVRSRDFH"
     gene            672..1049
                     /locus_tag="VGB88_12760"
     CDS             672..1049
                     /locus_tag="VGB88_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648368.1"
                     /translation="MTWDHVVALVDSLAWPAIVLVALFVLRRQAAAIAASIAARIRDP
                     RTTVTVGREGVTLQQAVAEAQARHDPIIDKSRTDPEFEERLRTWLKDNGIQASVTSFL
                     YGAQYEAARAKALADLIGEPKHT"
     gene            1056..1214
                     /locus_tag="VGB88_12765"
     CDS             1056..1214
                     /locus_tag="VGB88_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648369.1"
                     /translation="MEQKPKRIEKLTHVPVTLVAEIVEGFEKDGAVRIVTEREDDQHR
                     TVTAEFAS"
     gene            complement(1244..>2109)
                     /locus_tag="VGB88_12770"
     CDS             complement(1244..>2109)
                     /locus_tag="VGB88_12770"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9648370.1"
                     /translation="ITVERARRFYGPPEPVDRVEELTLPGPAGPLRARLYAAASDGPM
                     PVVVYFHGGGWVLGDVDSHDNGARALTNSAGALTVSIEYRRAPEHPFPAAVEDCLAAL
                     TWVAAHAGALGGDARRIAVAGDSAGGNLAAACALHSRDGAAPPLAFQLLIYPVLDSDL
                     DTPSYRAHAEAPMLTAERMAYFWRCYVPDAARRRDWRCAPLRAPELAGVAPAMIVASG
                     IDPLWSEGLAFAERLRAAGVAAEHVPFPRMTHAFFQAPSLLEDARAAIDRAGAALMRA
                     FATEPDRAAAK"
BASE COUNT          314 a          757 c          775 g          263 t
ORIGIN      
        1 gggcctgcgc ccttgcccct gttcgccgcc gccgaggccc gccacggcgc ggcgctcgcc
       61 ggccctgagc ccgcggtttc gctgcccgtg gcagggctcg gcgaggaggt cgccgacgac
      121 tatcggagcc tgcgcctgtc gctgaaggcg cacccgctgg cgctgctcag gaacgagctc
      181 ggccgccggc gggcggtgcc ggcggcgcgg ctcgcggaca tccgcgccgg ggcgcgcgtg
      241 cgcgtcgcag ggctggtgct ggtgcgccag cgcccgggca ccgccaaggg ggtgatcttc
      301 gccaccctcg aggacgagac cggcaccgcc aacgtcatcg tctggccgga cgtcttcgag
      361 cgcttccggc gcacggtgct ggcggcgaag ctgttgtgcg tcgagggcga ggtgcagcgc
      421 gagggcatcg tgctccacgt cgtcgccgcg cgcctggagg actggacgcc gctgctcggc
      481 cgcctcaccg agacacgaac cgagctgtcc ccggcgctgg cccgcgccga ccacgtcgcc
      541 cgccccggcc acgatccgcg caccctcccc gtccgcagcc gcgacttcca ctagatggac
      601 gcacagggca gccttgtcgg tttcttcggc acgaccgata ctcgcaggcc ggagatgacc
      661 acgaaccttc gatgacctgg gaccacgtcg ttgcactggt cgacagcttg gcctggccgg
      721 cgatcgtgct ggtggcattg ttcgtcctcc gccgccaggc ggcagcgatc gcggccagca
      781 tcgccgcccg gatcagggac ccgcgcacca cggtgaccgt cggacgcgaa ggcgtgaccc
      841 tgcaacaggc cgtggccgag gcgcaggcga ggcacgatcc gatcattgac aagagccgga
      901 ccgatcccga gtttgaggag cgcctcagaa cctggctaaa ggacaatggt atccaggcct
      961 ctgtgacctc gttcctgtat ggggctcaat acgaggcggc gcgggcaaag gcgctggcag
     1021 atctgatcgg tgagccgaaa cacacgtgaa cccaaatgga acaaaagccc aaaaggatag
     1081 aaaaactcac ccacgtgccg gtgaccctgg tcgccgagat cgtcgagggc ttcgagaagg
     1141 acggcgcggt caggatcgtc accgagcgtg aggacgacca gcaccgcacc gtgactgccg
     1201 agttcgcgag ctgagcccgc gccgatcgct ctccggcgaa gggttacttg gccgcggcgc
     1261 ggtcgggctc ggtcgcgaag gcgcgcatga gcgccgcccc ggcgcggtcg atcgcggcgc
     1321 gcgcatcctc gagcagcgac ggcgcctgga agaaggcgtg ggtcatgcgc gggaagggga
     1381 cgtgctccgc cgcgaccccg gcggcgcgca gccgctcggc gaacgcgagg ccttcgctcc
     1441 acagcgggtc gatgcctgag gcgacgatca tcgccggcgc gaccccggca agctcgggcg
     1501 cgcgcagcgg cgcacagcgc cagtcgcggc ggcgggcggc atccggcacg tagcagcgcc
     1561 agaagtacgc catgcgctca gccgtcagca tcggcgcctc ggcgtgggcg cgataggacg
     1621 gggtgtcgag gtcgctgtcg agcaccggat agatcaggag ctggaaggcg agcggcggcg
     1681 cggcgccgtc gcggctatga agcgcgcacg ccgcggcgag gttgccgccg gcgctgtcgc
     1741 cggcgaccgc gatccgccgc gcgtcgccgc cgagcgcgcc ggcatgggcc gcgacccagg
     1801 tgagcgccgc caggcagtcc tcgaccgcgg ccgggaacgg gtgctccggc gcgcgccggt
     1861 actcgatcga cacggtgagc gcgccggcgg agttggtcag cgcgcgcgcg ccgttgtcgt
     1921 ggctgtcgac gtcgccgagc acccagccgc caccgtggaa gtagaccacc accggcatcg
     1981 gcccgtcgct cgccgccgca tagagccgcg cgcgcaacgg gcccgccggg cccggcaggg
     2041 tcagctcctc gacccggtcg accggctccg gcgggccgta gaagcgccgc gcgcgctcga
     2101 ccgtgatcc
//
LOCUS       DASWHU010000342         4340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_210465, whole genome shotgun sequence.
ACCESSION   DASWHU010000342 DASWHU010000000
VERSION     DASWHU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210465"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGB88_12775"
     CDS             complement(<1..288)
                     /locus_tag="VGB88_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648371.1"
                     /translation="MTRRVHFVYDDREPVPEAIARIVGARRFGSILRNRTPLSETIAE
                     AARAAGVDEITIIEDTRDLERLRARLRAGPQDGCFVHFRARGAIADRDGLAL"
     gene            complement(285..1286)
                     /locus_tag="VGB88_12780"
     CDS             complement(285..1286)
                     /locus_tag="VGB88_12780"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9648372.1"
                     /translation="MRTRAAWRVDLGFALFAAFAVAAFVAGGYELRASALPAAAKAQG
                     WAWAPVIGAFAAYLGSHALRCLRLYVILLDFERSFARILRLYATLAVVGRLLPFKLGE
                     IFRFTELSHLLDSSRTALIAIATERWFDAVTLLAILVYGLLLDPGVAAGTSVLVVVLS
                     VVAVFIALAYRGLAGFARYLRLLAATRSRSARGLAALRLARLLDALSADVHRLLRGRA
                     MVLAVTSLAIWVLEIATMALLLSALAELSPSGFVGDLLRALDLLLGSGAAAGPSVQAY
                     FLVTLVAVGAAFIPLTVSYTLARVASWLAARRTSAEARRRYRRSPLPARAMRPRPAP"
     gene            complement(1297..2094)
                     /locus_tag="VGB88_12785"
     CDS             complement(1297..2094)
                     /locus_tag="VGB88_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9648373.1"
                     /translation="MPSSILLFIPAYNCAPQIGRTLAQIDARTAKLIDEVIVIDNRST
                     DATVAAARAAAARLDWGPVRILRNRENYGLGGSHKVAFAWALERAHSHLAVLHGDDQG
                     RIADLLPLIQAGAHESHDCLLGARFAAGSRLVGYAPTRILANRAFDLLFSLACGRRLY
                     DLGAGLNLYRTAILGDRFYARFPDDLTFNYCMMLAHAHLGHRFRFFPIEWREEDQVSN
                     VRALRQAARMLALLAGYVAGPDRFLAAEHRAVARASYPFDEVTDSGR"
     gene            complement(2189..3430)
                     /locus_tag="VGB88_12790"
     CDS             complement(2189..3430)
                     /locus_tag="VGB88_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX9648374.1"
                     /translation="MNRREVLLGGGSLLALAASGPAAWAEARKFDLVVKGGEVLDPSR
                     SLRARLDIGIRDGVIAALEDDIAPERAVRLLDASGNIVTPGLVDLHTHVFAYGSALGI
                     PPDELVPYTCTTTAVSAGDAGANNIAALRRFIAAQSRTRIYAFVNIANFGLAGFPVGE
                     LLTIDHAEVENCARAVASNPQFCIGVKVRESENVVGANGLEPLRRAIEAAEMAGPWAK
                     VMCHIGGAPGELSALLDLLRPGDILTHAYSGAGNNVVRDGTLLPAAKAAKERGVTIGV
                     GHGAGSFDYTVAEPAMAQGLMPDVISSDIHVLSGNSPGMPYLPWVMSKFLNLGMSLEQ
                     VVAAATVTPARVIDREPKLGTLEPGAPADIAVMALVEGPVTFEDTRRNLRRGSVHLAP
                     VATVRAGVPFGRPYMAPFSRG"
     gene            complement(3465..>4340)
                     /locus_tag="VGB88_12795"
     CDS             complement(3465..>4340)
                     /locus_tag="VGB88_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9648375.1"
                     /translation="PGDHVVCSWNPSCGHCFYCDRDLPILCEPFTRHQPRGHLLDGTS
                     RLSLGGRTLHHFSVVSSHAQYCVVPETGAIRVPAEIPFDRACLIGCGVMTGWGAVVNI
                     ARARPGASVAVIGCGAVGLNAVQAAALAGCEPVIAIDVADAKLEVARAFGATHALPAG
                     GDGPVDSRIKALTGGRGADYVIEAAGIANTLQLAFDCTRPGGDVVLLGKTDVDAKVAL
                     RWGSMMGERRMIRSSYGGARPRRDFPLLAKLYLEGKLKLDELITDRIALSDINRGFAA
                     MKQGRVIRAVVEMAH"
BASE COUNT          670 a         1487 c         1641 g          542 t
ORIGIN      
        1 cagggcgagg ccgtcgcggt cggcgatcgc gccgcgggcg cggaaatgga cgaagcagcc
       61 atcctgcggc ccggcgcgga ggcgggcgcg caggcgctcg aggtcgcgcg tgtcctcgat
      121 gatggtgatc tcatcgacgc cggcggcgcg cgcggcctcc gcgatggtct cgctgagcgg
      181 cgtgcggttg cgcaggatgc tgccgaaccg gcgggcgccg acgatgcgcg cgatcgcctc
      241 cggcaccggc tcgcggtcgt cgtagacgaa gtgcaccctc cgggtcatgg cgccggccgg
      301 ggacgcatcg cgcgcgccgg cagcgggctg cggcgatagc gccgccgcgc ctccgccgac
      361 gtccgccgcg cagccagcca cgacgcgacc cgcgccagcg tgtagctgac ggtgagcggg
      421 ataaacgccg cgccgactgc gaccagggtg accaggaaat aggcctgcac cgatggtccc
      481 gccgccgcgc cgctgcccaa cagcaggtcg agcgcgcgca acaggtcgcc gacgaagccg
      541 gacggcgaca gctcggccaa cgccgacagc agcagcgcca tggtcgcgat ctcgagcacc
      601 cagatggcga ggctggtgac cgcaagcacc atcgcccggc ccctgagcag gcggtggacg
      661 tcggcgctca aggcgtcgag caggcgcgcg agacggagcg cggcgaggcc gcgggcgctg
      721 cggctgcggg tcgcggcgag caggcgcagg tagcgcgcga accccgcgag cccgcgatag
      781 gcgagcgcta tgaacaccgc gaccacggac agcaccacca ccagcacgct ggtgccggcg
      841 gccacccccg ggtcgagcag caagccgtag accaggatcg cgagcagggt gacggcgtcg
      901 aaccagcgct cggtggcgat cgctatcagc gccgtgcgcg agctgtcgag gagatggctg
      961 agctcggtga agcggaagat ctcgccgagc ttgaacggca gcaggcggcc gaccacggcg
     1021 agcgtggcgt acagcctgag gatgcgggcg aacgagcgct cgaagtcgag caggatgacg
     1081 tagagccgca ggcatctgag cgcgtgtgag ccgagatagg cggcgaacgc gccgatcacc
     1141 ggcgcccacg cccacccctg cgccttggcc gcggcgggca gcgcgctggc gcgcagctcg
     1201 tagccgccgg cgacgaacgc ggcgacggcg aaagcggcga acagggcgaa gccgagatcg
     1261 acccgccagg ccgcgcgtgt gcgcacgatt ggcgtgctat cggccactgt cggtgacctc
     1321 gtcgaacgga tacgacgcgc gcgccacggc gcggtgctcg gcggccagga agcggtcggg
     1381 gccggcgacg tagccggcga gcagcgccag catgcgcgcc gcctggcgga gcgcgcgcac
     1441 gttcgagacc tggtcctcct cgcgccattc gatcgggaag aacctgaacc ggtggccgag
     1501 atgcgcgtgc gcgagcatca tgcagtagtt gaaggtgagg tcgtcgggaa agcgcgcata
     1561 gaaccgatcg ccgagaatcg cggtgcgata gaggttgagc cccgcgccca gatcatacag
     1621 ccttcgcccg caggcgagcg agaacagcag gtcgaaggcg cgattggcga ggattcgcgt
     1681 cggcgcatag ccgaccaggc gcgagccggc ggcgaatcgc gcgccgagca ggcagtcgtg
     1741 gctttcgtgc gcacccgcct ggatcagcgg caacagatcg gcgatgcggc cctggtcgtc
     1801 gccatggagc accgcgaggt ggctgtgcgc gcgctcgagc gcccaggcga aggcgacctt
     1861 gtgcgagccg ccgaggccgt agttctcgcg gttgcgcagg atgcgcaccg gcccccagtc
     1921 gagccgcgcg gcggcggcgc gcgcggccgc gacggtggcg tcggtcgagc ggttgtcgat
     1981 gacgatcacc tcgtcgatca gcttggccgt gcgcgcgtcg atctgggcga gcgtgcgccc
     2041 gatctggggc gcgcagttgt aggcggggat gaacaacagg atcgatgaag gcatgtccgg
     2101 gggccgctgg ctgcgctggg ccccaatgaa ggtggtcgat gcgccgccct ttttccagcg
     2161 aggcgcgggc ttcgcggtcg ccgcccgctc agccgcgcga gaacggcgcc atgtacgggc
     2221 ggccgaaggg caccccggcg cgcacggtcg cgaccggcgc cagatggacg ctgccccggc
     2281 gcagattccg gcgcgtgtcc tcgaaggtca ccggtccctc gaccagcgcc atgaccgcga
     2341 tgtcggccgg cgcacccggc tccagggtgc cgagcttcgg ctcgcggtcg atcacccggg
     2401 cgggggtgac ggtggccgcc gccactacct gctcgagcga catgccgagg ttgaggaact
     2461 tgctcatcac ccaaggcagg tacggcatgc ccggcgagtt gccggacagg acgtggatgt
     2521 cgctcgagat cacgtcgggc atcaggccct gggccatggc cggctcggcg acggtgtagt
     2581 cgaacgagcc cgcgccgtgg ccgacgccga tcgtcacccc gcgctccttg gcggccttgg
     2641 cggccggcag cagcgtgccg tcccggacga cgttgttgcc ggcaccgctg taggcgtggg
     2701 tcaggatgtc gcccggcctg agcaggtcga gcagcgccga cagctcgccc ggcgcgccgc
     2761 cgatgtggca catcaccttg gcccacgggc ccgccatctc ggccgcctcg atcgcgcgcc
     2821 tgagcggctc gagcccgttg gcgccgacca cgttctcgct ctcgcgcacc ttgacgccga
     2881 tgcagaactg cgggtttgag gcgacggcgc gggcgcagtt ctcgacctcg gcgtggtcga
     2941 tggtcagcag ctcgccgacc gggaagccgg cgaggccgaa attggcgatg ttgacgaacg
     3001 catagatgcg ggtgcgcgac tgggcggcga tgaagcgccg gagggcggcg atgttgttgg
     3061 cgcccgcgtc gccggccgag acggcggtgg tcgtgcaggt gtaggggacg agctcatcgg
     3121 gcgggatgcc gagcgccgag ccgtaagcga acacgtgggt gtggaggtcg accaggccgg
     3181 gggtgacgat gttgcccgag gcgtcgagca gccgcaccgc gcgctccggc gcgatgtcgt
     3241 cctcgagcgc ggcgatcacg ccgtcgcgga tgccgatgtc gagcctggcc ctcaggctcc
     3301 ggctcggatc gagcacctcg ccgcccttga cgacgagatc gaacttacgc gcctcggccc
     3361 acgccgccgg gcccgaagcg gccagcgcca gcagcgagcc gccgcccaac aagacctcgc
     3421 gccggttcat atcgtgcctc ccatcccgcg cattccccat cgatctaatg cgccatctcg
     3481 accacggcgc ggatcacgcg gccctgcttc atggcggcga agccgcggtt gatgtcggaa
     3541 agcgcgatcc gatcggtgat cagctcgtcg agcttgagct tgccctcgag atagagcttg
     3601 gcaagcagcg ggaagtcgcg cctggggcgg gcgccgccgt aggacgagcg gatcatcctg
     3661 cgctcgccca tcatcgagcc ccagcgcagc gccaccttgg cgtccacgtc ggtcttgccg
     3721 agcaggacca cgtcgccgcc ggggcgggtg cagtcgaagg cgagctgcaa ggtgttggcg
     3781 atgccggcgg cctcgatcac gtagtcggcg ccgcgcccgc cggtgagcgc cttgatgcgc
     3841 gaatcgaccg ggccgtcgcc gccggcgggc agcgcatggg tggcgccgaa ggcgcgcgcg
     3901 acctcgagct tggcgtcggc gacgtcgatc gcgatcaccg gctcgcagcc ggcgagcgcg
     3961 gcggcctgga ccgcgttcag cccgaccgcg ccgcagccga tcaccgccac ggacgcgccg
     4021 gggcgggcgc gggcaatgtt gaccaccgcg ccccagccgg tcatcacccc gcagccgatc
     4081 aggcaggcgc ggtcgaacgg gatctcggcg ggcacgcgga tcgcccccgt ctcgggcacc
     4141 acgcagtact gggcgtgcga ggagacgacc gagaagtggt gcagcgtccg cccgccgagg
     4201 ctgagccgcg aggtgccgtc gagcagatgg ccgcgcggct gatggcgggt gaacggctcg
     4261 cacaggatcg gcaggtcgcg gtcgcaatag aagcagtggc cgcagctcgg gttccacgag
     4321 cacaccacgt ggtcgccggg
//
LOCUS       DASWHU010000343         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_954283, whole genome shotgun sequence.
ACCESSION   DASWHU010000343 DASWHU010000000
VERSION     DASWHU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954283"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VGB88_12800"
     CDS             complement(<1..1568)
                     /locus_tag="VGB88_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX9648376.1"
                     /translation="MSDLGFLATVLIFLAAGVIVAPIFQRLRSSLVLGYLVAGVIIGP
                     HVLGLVANEERTRGIAELGVVALMFTIGLELTIDRLRVVRRHVFGVGTTQVLVTALVI
                     GVIAALAGAEPRAAILIGGALAMSSTAVVLQILAERGGLATRVGRVAFAVLLLQDMAV
                     VPLLALVPALAGPESQLVPTVLETLAKAAVALVAIFALGRLVLRPVFRIFAIGAVPEL
                     FAAVTLLVLLGTSFATHEFGLSLGIGGFLAGILLAETEYRHQVAADIQPFRGLLLGLF
                     FMTVGMAVDLPLIAQQVWPIVGLTVALIVVKSALLALLCRGSGLPWGASIHAGLLLGQ
                     GSEFAFVVIGSAAAVRLIDPAVGDFLVVVVALTMAATPLLAAMGQRLDRRFEVHRVNT
                     TQHVEEETSDLKDHAIVAGFGRVGQSVSRMLNAAGQPYVAFELDANRVAAARRHGLPV
                     YYGDASRVEVLRAAGAGRARTAVIILDNAAAAENTVSVLHHNFPAVKTFVRARDHRHR
                     RRLEAVGAQNVVHET"
BASE COUNT          290 a          550 c          590 g          178 t
ORIGIN      
        1 gtctcgtgga cgacgttctg cgcgccgacc gcctccaacc ggcggcgatg gcggtgatcg
       61 cgcgcccgca cgaaggtctt cacggcgggg aaattgtggt gcagcaccga cacggtattc
      121 tccgccgcgg cggcattgtc gaggatgatc acggcggtgc gcgcccgccc cgcgccggcg
      181 gcgcgcagca cctcgacgcg gctcgcgtcg ccataataga ccggcagtcc gtgacgcctg
      241 gccgcggcga cgcggttcgc gtcgagctcg aaggcgacgt agggctggcc ggccgcgttc
      301 agcatgcgcg acaccgactg cccgacccgg ccgaacccgg cgacgatcgc atgatccttg
      361 aggtcggagg tctcctcctc gacgtgctgg gtcgtgttga cgcgatggac ctcgaaccgc
      421 cggtcgaggc gctggcccat cgcggcgagg agcggcgtcg ccgccatggt gagggcgacg
      481 acgacgacaa ggaagtcgcc gaccgccgga tcgatcagcc gcaccgcggc cgccgatccg
      541 atgaccacga aggcgaattc gctgccctgg ccgagcagca acccggcatg gatcgaggcg
      601 ccccacggca ggccggagcc gcggcacagg agcgcgagga gcgccgactt gacgacgatg
      661 agggcgacgg tcaagccgac gatcggccag acctgctggg cgatcaacgg caaatcgacc
      721 gccatgccga cggtcatgaa gaacagcccg agcaagaggc cgcggaacgg ctggatgtcg
      781 gccgcgacct gatggcgata ctcggtctcg gcgaggagga tgccggcgag gaacccgccg
      841 atgccgagcg agagcccgaa ttcgtgggtg gcgaagctgg tgccgaggag aacgagtagc
      901 gtcaccgccg cgaacagctc gggcaccgct ccgatcgcga agatgcggaa caccgggcgc
      961 agcaccagcc ggccgagggc gaagatcgcg accagcgcga ccgccgcctt ggcgagcgtc
     1021 tcgagcacgg tcggcacgag ctgggattcg ggaccggcga gcgccggcac gagagcgagc
     1081 agcggcacga ccgccatgtc ctgcagcagg agcaccgcga atgcgacgcg gccgacgcgg
     1141 gtcgcgagcc cgccccgctc cgccaggatc tgcagcacca cggcggtcga ggacatggcg
     1201 agcgcgccgc cgatcaggat cgccgcgcgc ggctcggcgc cggcgagcgc cgcgatgacg
     1261 ccgatgacga gcgccgtcac caggacctgg gtcgtgccca ccccgaacac gtggcggcgc
     1321 acgacgcgga gccggtcgat ggtgagctcg agcccgatgg taaacatgag ggcgacgacg
     1381 cccagctcgg cgatgccacg cgtgcgctcc tcgttggcga ccagtcccag cacgtgcggc
     1441 ccgatgatca cgccggccac caggtagccc agcaccaggc tcgaccgcag ccgctggaag
     1501 atcggcgcga cgatcacgcc cgcggcgaga aagatcaaga cggtggcgag gaacccgaga
     1561 tcggacatgg gatatgtggc gggccgccgg atgcggccgt gtcgcaat
//
LOCUS       DASWHU010000344         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3529056, whole genome shotgun sequence.
ACCESSION   DASWHU010000344 DASWHU010000000
VERSION     DASWHU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3529056"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGB88_12805"
     CDS             complement(<1..181)
                     /locus_tag="VGB88_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648377.1"
                     /translation="MAAAPLRASACRLMMAARDLPAYPEGPGWRDNEASIAAAEAMAA
                     DAPTIRARVLRAIAGA"
     gene            complement(240..383)
                     /locus_tag="VGB88_12810"
     CDS             complement(240..383)
                     /locus_tag="VGB88_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648378.1"
                     /translation="MSKNTVRRYLRPPGHPEHRRPPDEVKLRIAEATDGEVPVSAWFE
                     AAE"
     gene            535..1401
                     /locus_tag="VGB88_12815"
     CDS             535..1401
                     /locus_tag="VGB88_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9648379.1"
                     /translation="MIFPGPPSPDPAPPAMLARMAKLLRLPGRSPCPPAGHDPPGRTP
                     GPPHPNRIALLARRKGWKQKHLVEATGLSQQAVSRYWRGERPLRTDTLDLFAAALGCR
                     PAAILAGDEDTPSVPETMIVAAAFAEAGPGPGGFDLAPPWRMREVLPGLAHTGQCFAA
                     RVADNSADGFYPPGSLLVACPLEALTGPLCAGDMVLVRRRDATGHTMEILAGMLALTP
                     DGELLVRIRSSNAEVSRVVVLGTLPQAGRMAESAAPWREAGAGAAIGYAPEPGDRDEI
                     IARIEQKFTAQS"
     gene            1802..2047
                     /locus_tag="VGB88_12820"
     CDS             1802..2047
                     /locus_tag="VGB88_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648380.1"
                     /translation="MPGSGLELSEELSHVEHFLIRNFRSLRGGQRRAVADIVGDLARA
                     ELARAEPHTRGQLCGPMTAALRAKGGHVHRSLEGEGG"
     gene            2092..2268
                     /locus_tag="VGB88_12825"
     CDS             2092..2268
                     /locus_tag="VGB88_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648381.1"
                     /translation="MAAPKPRASVKVTVTTVGGTPLGSALLAGAGDSALELAADVLAL
                     IAAHYDAVEEPRRS"
     gene            2528..2761
                     /locus_tag="VGB88_12830"
     CDS             2528..2761
                     /locus_tag="VGB88_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648382.1"
                     /translation="MPRRGPARMTVIRWLGTALLLGGTLALAAGWPQWHTAAFGMRVA
                     GDACWLAAAVVRADAALAVTIGGFLLIDLWGAS"
     gene            3036..3413
                     /locus_tag="VGB88_12835"
     CDS             3036..3413
                     /locus_tag="VGB88_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648383.1"
                     /translation="MTPGMVHYPSGRSVVMTDLGPLDELARVRTASPCFDVRVLCSGA
                     KTSLVSDGRRTWWIESWRLTRTGVQRAAEAAVDRAAANHAALRAYRNGFDDTLRLTTR
                     TGDAIELRRGDHPEPPSDTPWPA"
     gene            3439..3687
                     /locus_tag="VGB88_12840"
     CDS             3439..3687
                     /locus_tag="VGB88_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9648384.1"
                     /translation="MPAAARHIDHAILAELAATGPLTAAEAGARLGVSRQRAARALEL
                     MRRRLLVTIAAYRLPPGHRGRAMKRATPVYAAGESADA"
     gene            3680..>3991
                     /locus_tag="VGB88_12845"
     CDS             3680..>3991
                     /locus_tag="VGB88_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9648385.1"
                     /translation="MPDRRAVAQAGPLDDARLEALLVGHVAILGRTGSGKSYVAKGWV
                     ERLLRLGRRVCVIDPTSVWWGLKSSADGKAPGFPVAVLGGDHADIALTETAGELLGRT
                     VA"
BASE COUNT          513 a         1518 c         1437 g          523 t
ORIGIN      
        1 aggcgccggc gatcgcgcgc agcacgcggg cgcggatggt cggtgcgtcc gccgccatcg
       61 cctcggcggc ggcgatcgag gcctcgttgt cgcgccagcc cggcccctcg ggataggcgg
      121 gaaggtcgcg ggcggccatc atgagacggc acgcggaggc gcggagtggt gctgcagcca
      181 ccctccgcgc ctccgcgtgc accaccgcgt tgcctctgac ggcctgcggg actggatgct
      241 cattcggccg cctcgaacca ggccgagacc ggcacctcgc cgtccgtcgc ctcggcgatc
      301 ctgagcttga cctcgtcggg cgggcggcgg tgctcgggat ggcctggcgg ccgcaggtag
      361 cggcggaccg tgttcttcga cacgcccagc gcctcggtgc cgaaacgctc cgggctgaga
      421 cctttccctc gcaaccagtc ggccaatgtt tgcaagatgc ggcggtctcc gaggccaacg
      481 cctaccaaat gtggggtctg gccctgatac cccgtttcgg ggtatgatgc aaccgtgatt
      541 ttccccggcc cgccgtcgcc cgatccggcg ccaccggcca tgctcgcgcg catggcgaag
      601 ctgttgcgcc ttcccggccg cagcccgtgt ccgccggccg gccacgaccc gccggggcgc
      661 acgccgggcc cgccgcaccc caaccgcatc gccctgctcg cgcgccgcaa gggctggaag
      721 cagaagcacc tggtcgaggc gaccgggctg tcgcagcagg cggtgtcgcg ctactggcgc
      781 ggcgaacggc cgctgcgcac cgacacgctc gatctgttcg ccgccgccct gggctgccgg
      841 ccggcggcga tcctggccgg ggacgaggac accccgtcgg tgcccgagac catgatcgtc
      901 gccgcggcct tcgccgaggc cggacccggc ccgggcgggt tcgatctcgc cccgccctgg
      961 cgcatgcgcg aggtgctgcc gggcctcgcc cacaccggcc agtgctttgc cgcgcgcgtc
     1021 gccgacaaca gcgccgacgg gttctacccg ccgggctcgc tgctcgtcgc ctgcccgctc
     1081 gaggcgctga ccggcccgct gtgcgccggc gacatggtcc tggtccggcg ccgcgacgca
     1141 acggggcaca ccatggagat actggccggc atgctcgcgc tgacccccga cggcgagctc
     1201 ctggtgcgca tccgctcgtc caacgccgag gtgagccggg tcgtcgtgct cggcaccctg
     1261 ccccaggcgg ggcgcatggc ggaatcggcc gcgccgtggc gcgaggccgg cgccggcgca
     1321 gccatcggct acgcgcccga gcccggcgac cgcgacgaga tcatcgcccg gatcgagcag
     1381 aagttcaccg cccagtcgta gcgccccccg cttccgcggg ggcaggctcg gccgcgccgg
     1441 cgccgagaac cgcccgcgcc gggcggtttt ccgcacccct acgaatagcc cttgaccgaa
     1501 taccccgaac tggggtatcc gggttgcgtt cgccaccggt ccttccctcc ctgggccggc
     1561 ggcggccgcc gaagccgggg cggcgtgggg acgcccggca cgccctgcgg agcgcggtac
     1621 gcgcgaagca gggacatgga gcggaacgga gcagcccggc cggcggcaac cttcggaccc
     1681 gagagccgaa ggccctgcac ctgagccacg aagccccgcc tgccttcgcc gaagcgaagc
     1741 tgcttcggct tcgcgcaggc aggccgccgg ccgggcatcc ggaccaccga aggacgcatc
     1801 gatgcccgga tccggcctag agctgagcga agagctgagc cacgtggagc acttcctgat
     1861 ccgcaacttc cgctcattgc gcggcggcca gcgccgcgcg gtggccgaca tcgtcggcga
     1921 cctggcgcgc gccgagctgg cccgcgccga gccccacacc cgcggccagc tctgcggccc
     1981 gatgaccgcg gcgcttcgag ccaaaggcgg acacgtccac cgtagcctgg aaggcgaagg
     2041 cggatgacgg cggcgctcgg cagcactcat gcccaaccgg cgccacgcgt cgtggcagcg
     2101 ccaaagccgc gcgcctcggt caaggtcacg gtcaccaccg tcggcggtac gccgctcggc
     2161 tcggcattgc tcgccggggc gggcgacagc gcgctcgagc tcgccgcgga cgtgctggcc
     2221 ctgatcgccg cccactacga cgcggtcgag gagccgaggc ggtcatgatc gccgcctgtg
     2281 gcacgacgtt ggtcgcgagt cggtcacgcg gcgcccccgg gtctccgccc ggggcaggcg
     2341 cggagagcat gcggggtgcc agtgcggcgg accgtggtgc gcgcagcgcg cgtgcccagg
     2401 agaccatccg tccgctcccg gaggcagcat ccgggccccg ctccatcctg gtctacgacg
     2461 tccgccgcga gctcacagcc accctgtggg acgccgccgg cccgccgccg gccgaggacg
     2521 cgcggccatg ccgcgcaggg ggccggcgcg catgacagtc atccgctggc tcggcactgc
     2581 gctgctgctc ggcggcacgc tcgcgctcgc cgccggctgg ccgcagtggc acacggccgc
     2641 tttcgggatg cgcgtcgcgg gcgatgcgtg ctggctcgcc gccgcggtgg tgcgcgccga
     2701 cgccgcgctc gccgtcacca tcggcgggtt cctgctgatc gacctgtggg gcgcgtcgtg
     2761 agtgcggatc gggcacgcgg agacgcggag ggcctgctcc cgaccgaatc gggagtcgcg
     2821 gatatcgttc tcgccgcgcg gcatgcgctc gcctgggccg atcgcgtcga acctcctgag
     2881 ggcgacgatg ccgctctcga agccgccaac gattggctga acgaaggcgg cggctgggac
     2941 gatctacgca ccgtgcaaac agcacttcgg attgcactcg agcccttctc cgcggctccg
     3001 cgtgccccag gccttggcgg acgaaaggag ccccgatgac ccccggaatg gtccactacc
     3061 catcgggccg gtcggtggtg atgaccgacc tcggcccgct tgacgagctt gcccgcgtgc
     3121 gcaccgcgag cccctgtttc gacgtgcgcg tgctgtgcag cggcgccaag acgtctctgg
     3181 tcagcgacgg gcggcgcacc tggtggatcg agtcctggcg gctcacccga accggggtcc
     3241 agcgcgccgc cgaggccgcg gtcgacaggg cagcggccaa ccatgccgcg ctgcgcgcct
     3301 accggaacgg cttcgacgat acgcttaggc tgactacccg gacgggcgac gccatagagc
     3361 ttaggcgagg cgatcacccc gagccgccat ccgacacgcc gtggccggca tgaccctcga
     3421 cgccgcgtcc ccgttctgat gcccgcagcg gcgcgccata tcgaccacgc catcctcgcc
     3481 gagctcgcgg caacggggcc gctgaccgcc gccgaagccg gcgcgcgcct cggcgtgtcg
     3541 cgccagcgtg cggcgcgcgc tctcgagctg atgcgccgcc ggctgctggt cacgatcgcg
     3601 gcgtaccggc tgccgcccgg ccaccgcggg cgcgcgatga agcgcgccac gccggtttat
     3661 gcggcgggcg agtccgctga tgcctgaccg ccgcgccgtc gcccaggcgg gtccgttgga
     3721 cgatgcccgg ctcgaggcgc tgctcgtcgg ccacgtcgcc atcctgggcc gcaccgggtc
     3781 gggcaagtcc tacgtcgcca agggctgggt ggagcgcctg ctgcggctcg gccggcgggt
     3841 gtgcgtgatc gacccgacat ctgtgtggtg ggggctcaag tcgagcgccg acggcaaggc
     3901 gccgggcttc ccggttgcgg tgctcggcgg cgaccacgcc gacatcgctc tcaccgagac
     3961 cgccggcgag ctgctgggcc gcaccgtcgc c
//
LOCUS       DASWHU010000345         4670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_956084, whole genome shotgun sequence.
ACCESSION   DASWHU010000345 DASWHU010000000
VERSION     DASWHU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956084"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="hisG"
                     /locus_tag="VGB88_12850"
     CDS             complement(<1..665)
                     /gene="hisG"
                     /locus_tag="VGB88_12850"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX9648386.1"
                     /translation="MADAGKLVIAVPKGRILDELVPLLARAGIEPEPAFAEPESRQLA
                     FATSQPGVTLIRVRSFDVATFVAFGAAQLGVAGRDVLMEFDYPELYAPLDLRIGRCRL
                     SVAEPAELASGDDPRRWSHVRVATKYPETTRRHFAARGVQAECIKLNGAMELAPRLGL
                     CRRIVDLVSTGNTLKANGLVEVEKIADVSARLIVNRTAWKTRPGDLGRWLDRLREVVH
                     GAAA"
     gene            complement(665..1129)
                     /locus_tag="VGB88_12855"
     CDS             complement(665..1129)
                     /locus_tag="VGB88_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX9648387.1"
                     /translation="MRALTPSLLRVRAVEAGDYEVLAACLQDALMPLAEMAFRPRERR
                     FVAVFDRFMWEHCAPLTAERRPPFVVQSALRVEGVLRTRSTGIELTARDRLLELLTVA
                     SEPGALVLVFAGGAAIRLEGERLACVVEDLAQPRPARAVPRHRLGAGGEEGA"
     gene            complement(1126..2400)
                     /gene="murA"
                     /locus_tag="VGB88_12860"
     CDS             complement(1126..2400)
                     /gene="murA"
                     /locus_tag="VGB88_12860"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9648388.1"
                     /translation="MDKIRIRGGRPLSGRITIGGAKNAALPLLAACLLTDETVELGNL
                     PRVADIDTMSHLLESLGVEAATADGAGGRPGLAVRARTLRDHTAPYDLVRRMRASVLV
                     LGPLLARAGRARVSLPGGCAIGPRPVDLHLQGLRQLGAEIALDDGYIDARAPAGLRGA
                     HISLPSVSVGATENLIMAAALARGTTTLANAAREPEVVDLANCLAAMGADIAGAGSDT
                     ITVTGTDRLHGTRYDVLPDRIETGTYLVAAAITGGELELAGARLDLIDAVAETLAAAG
                     VELRESAHGLRVRRRDSAIAGFDVMTAPYPGFPTDMQAQMMSLMAIADGASLITETIF
                     ENRFMHVPELARMGANIVVHGGSAMVRGVPALTGAPVMATDLRASVSLVLAGLAAREE
                     TVVNRVYHLDRGYERVEEKLAACGADIERIRA"
     gene            complement(2563..2781)
                     /locus_tag="VGB88_12865"
     CDS             complement(2563..2781)
                     /locus_tag="VGB88_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648389.1"
                     /translation="MANKIEPVGGSANSTQGQTGRGRFQKLAEALRANLRKRKEQERA
                     RRDQADRPSGEDDPNLVPSPPHITDFEA"
     gene            2973..3047
                     /locus_tag="VGB88_12870"
     tRNA            2973..3047
                     /locus_tag="VGB88_12870"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3005..3007,aa:Thr,seq:ggt)
     gene            3402..3596
                     /locus_tag="VGB88_12875"
     CDS             3402..3596
                     /locus_tag="VGB88_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648390.1"
                     /translation="MKLMYAALIHAAEPWRGLKMTEFERRQLQAIRDELDRAHAERIA
                     PAVRANVTASQPRLSSKERT"
     gene            3598..4448
                     /locus_tag="VGB88_12880"
                     /pseudo
     CDS             3598..4448
                     /locus_tag="VGB88_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251879.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          712 a         1694 c         1584 g          680 t
ORIGIN      
        1 gccgccgcgc catgcaccac ctcgcgcagc cggtcgagcc agcggccgag gtcccccggg
       61 cgggtcttcc aggcggtccg gttgacgatc aggcgcgccg agacgtcggc gatcttctcg
      121 acctcgacca ggccgttggc cttgagcgtg ttgccggtcg agaccaggtc gacgatgcgc
      181 cggcacaggc cgagccgcgg cgccagctcc atcgcgccgt tgagcttgat gcactccgcc
      241 tggacgccgc gcgcggcgaa atggcggcgc gtggtctcag gatacttggt cgccacccgc
      301 acgtggctcc agcgcctggg atcgtcgccc gaggcgagct cggccggctc ggcgaccgac
      361 agccggcagc gcccgatacg caggtcgagc ggcgcgtaca gctcggggta gtcgaactcc
      421 atcagcacgt cgcgcccggc gacgccgagc tgcgcggcac cgaaggcgac gaaggtggcg
      481 acgtcgaagc tgcgcacgcg gatcaaggtg accccgggct ggctggtggc aaaggcgagc
      541 tgacgcgact cgggctcggc gaacgcgggc tcgggctcga tgccggcgcg ggccaagagt
      601 ggcaccagct cgtcgaggat gcggcccttc ggcaccgcga tcaccagctt gccggcatcg
      661 gccatcacgc gccctcctcc ccgcccgcgc ccaggcgatg gcgaggcacc gcgcgcgccg
      721 gccgcggctg cgccaggtcc tcgacgacgc acgccagccg ctcgccctcg agacggatcg
      781 ccgccccgcc cgcaaacacc agcaccagcg cgcccggctc gctggcgact gtcaggagct
      841 caagcaaccg atccctcgcc gtcagctcga tgccggtcga gcgcgtccgc aacacgccct
      901 cgaccctgag cgccgactgg accacgaagg gcgggcgccg ttcggcggtg agcggcgcgc
      961 aatgctccca catgaagcgg tcgaacacag cgacgaaccg gcgctcgcgc ggccggaacg
     1021 ccatctcggc gagcggcatc agggcatcct gaaggcacgc cgccagcacc tcgtagtcgc
     1081 cggcctcgac cgcgcgcacg cgcaacaggc tcggcgtcag ggctctcatg cgcggatccg
     1141 ctcgatatcg gcgccgcagg cggcgagctt ctcctcgacc cgctcgtagc cgcggtcgag
     1201 atggtacacc cggttgacca cggtctcctc gcgcgccgcg agcccggcca gcaccagcga
     1261 gacgctggcg cgcaggtcgg tcgccatcac cggcgcgccg gtcagcgccg gcacaccgcg
     1321 gaccatcgcc gagccgccgt ggacgacgat gttcgcgccc atgcgcgcca gctcgggcac
     1381 gtgcatgaac cggttctcga agatcgtctc ggtgatcagg gaggcgccgt cggcgatcgc
     1441 catcagggac atcatctgcg cctgcatgtc ggtggggaac ccggggtaag gcgcggtcat
     1501 gacgtcgaac ccggcgatcg ccgagtcgcg ccggcgcacg cgcaggccgt gcgcgctctc
     1561 gcgcagctcg acaccggccg cggcgagcgt ctcggcgacc gcatcgatca ggtcgagccg
     1621 cgcgcccgcg agctcgagct cgccgccggt gatcgccgcc gcgaccagat aggtgccggt
     1681 ctcgatgcgg tccggcagca cgtcgtagcg ggtgccgtgc aggcggtccg tgccggtgac
     1741 ggtgatggtg tccgagccgg cgcccgcgat gtcggcgccc atcgcggcca ggcaattggc
     1801 gaggtcgacc acctcgggct cgcgcgcggc gttggcgagc gtggtggtgc cgcgggcgag
     1861 cgcggccgcc atgatcaggt tctcggtcgc gccgaccgac accgagggca gcgagatgtg
     1921 cgcgccgcgc aagcccgcgg gcgcgcgggc gtcgatatag ccgtcgtcga gcgcgatctc
     1981 ggcgccgagc tggcgcaggc cctggaggtg caaatcgacc gggcgcgggc cgatcgcgca
     2041 gccgccgggc agcgacaccc gcgcccgccc cgcgcgcgcc agcagcggcc ccagcaccag
     2101 caccgaggcg cgcatccggc gcaccaggtc gtagggcgcg gtatggtcgc ggagggtgcg
     2161 ggcgcgcacc gccagcccgg gccggccgcc ggcgccgtcg gccgtggccg cctcgacgcc
     2221 caggctctcc agcaggtgcg acatggtgtc gatgtcggcg acccgcggca ggttgccgag
     2281 ctcgaccgtc tcgtcggtga gcaggcaggc ggcgagcaac ggcagcgccg cgttcttcgc
     2341 gccaccgatg gtgatccgtc ccgacagcgg ccggccgccg cgaattcgta tcttgtccat
     2401 gttcgatcgc tgccgcgagc cgcccgctgc ggcggattgg gcgccaaccg cgccgatgcc
     2461 cgttcaagat gtccgacgcg cccgggccgc gcccgccccg gcgcgcgcgg cggaggtatt
     2521 tataccagcg ccggagtctt ctcaacctgg ccgcgaccgc gctcaggcct cgaagtcggt
     2581 gatgtggggc ggcgagggca ccaggttcgg atcgtcctcg ccggagggcc ggtcggcctg
     2641 gtccctgcgc gcgcgttcct gctccttgcg cttgcgcagg ttcgcgcgca acgcctcggc
     2701 gagcttctgg aaccggccgc ggccggtctg gccttgggtc gaattcgccg agccgccgac
     2761 cggctcaatc ttgttggcca tcgacctgct cccgtccgcg tcccgaccgg gacccgggcg
     2821 tccgcgccta gactagcgca agtgcggccg gtgccgaaac cttgccgaac atgcgcgccc
     2881 cccgccccct gcgcttgcag gcccgagggg gctgtggcat ggtccgccct gcggcccaga
     2941 gcctacgggc gaccgggccg cccgcgccga ccgccgccgt agctcagagg tagagcacac
     3001 gcttggtaag cgtggggtcg gtggttcgat tccacccggc ggcaccattc caaatcaaca
     3061 gattatcaga tgtttttcta gtgacgaact agacgattcg gtgggattgg taacagtatt
     3121 ggtaacagaa ttgcagtcgg ccgggccggg cgacacggaa gtgccgggcc cttaagagcc
     3181 ggacgccgcc tggaggtcgg tcaccagtcc ttcactcggt cgcccgggac tccgccagcg
     3241 ccgctggctg gtcagtaccc ctgccaagcg cgcgggcatt ccgcaacctt agttgcccca
     3301 atgcgaacgg cgtggcggaa tcgcccgcct cgacgtgctc gcagaaacag cccgccacgt
     3361 tatcgagata cgcaccacat gccttcggcg agcgtgcggt cttgaagctg atgtatgccg
     3421 cgctcatcca cgccgccgag ccttggcggg gcctcaagat gaccgagttc gagcgccgtc
     3481 agctgcaagc gatccgtgac gagctagacc gcgctcacgc cgagcgaatc gcgccggccg
     3541 tgcgcgcaaa cgtcaccgca tcccagcccc gtttatccag caaggagcgg acttaacctg
     3601 ccgcgctacc agaccgatct gaccgacacg gaatggcgcc tgatcgagcc ccacctgccg
     3661 ccgacgaaga ccacggggcg gccacgcgcc tggccgatgc gggagatcgt caacgccaac
     3721 ttctacgtga tgcgcggcgg catagcctgc cgccttctgc ccttggactt cctgccctgg
     3781 ggcacagtct atcgcgggct tgcgacctgg cgggacgacg gcgtcttcga gcggatcaac
     3841 catgctccgg tgatcgccga ccgcgagcgg gtcgggcgcg aggcgagccg ttcagccgcg
     3901 atcatccccg gattaaaccc ggggaccagc cagagcgtaa agaccaaaga ggcgggaggc
     3961 ccgcgcgacg gagcctgtcc tcgggccagc cttcggtcgg acccggggaa cgcgggcaag
     4021 aagatcaatg gccgcaagcg acacgcgctc gtcgacaccg acggccgggc attggtcgca
     4081 gtgccgcttc cgactaatat ccagaaccgt gacggcggcg ggccgctgct cgaggcctcg
     4141 cgccccatct tcccgttcat cgaacgcgtc ttcgcccatg ccgcatacga tcacgagcgg
     4201 gtcaccgccg ccaccaccgt cgccgtcgag atcgtccgca agctccccga tcagctcggc
     4261 tttatcgtgc tggcgcgccg ttgggtggtc tagcgtttct tcgcttggat cggccgaaac
     4321 cgcaggctcg ccaagagcct gccccggcga aggccgggga cttcgaggcg accattgcgt
     4381 ctgcacgcgg cttcctctac gccgcctctg tcatgctgct cgttcgccgc ctcgccaaag
     4441 cttcatgagt ttcgaaaccg cctctcagtc atccccgcct cgccggcgcc ggaacccttg
     4501 tcaggctacc ggatcacacg acgtggcacc gagcctgggg acttttcaga ggtttcctaa
     4561 ggatcgttgc ctcaactccc agacaatttt atagaattcg atggcgtctt cataagttgc
     4621 catcaaatat tgtctcgggc tcttctcagc ttgcatgagc acttcgacga
//
LOCUS       DASWHU010000346         9277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1321212, whole genome shotgun sequence.
ACCESSION   DASWHU010000346 DASWHU010000000
VERSION     DASWHU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321212"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VGB88_12885"
     CDS             complement(<1..1001)
                     /locus_tag="VGB88_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9648391.1"
                     /translation="MQAAQDRWRAELEAQPVAFMLDRLAPALRQAAAELAAFLGAEAG
                     DLALVANATEGANAVLRAVDLGPGDVVLATSGAYPAVRNTLSHLCRERGATLHVVALP
                     TPLAGPEAAVEPVVAALDRGARLAVLDHVVSASGTVLPIAALCRACRERGVAVLVDGA
                     HAPGMLDLDVPAIGADWYVGNCHKWLCAPKGAGFLWARKDRRERLHPAVISNLYGAGF
                     PTEFEWTGTRDYSAVLAVPAALAFWRELGPEAARANMAGLARQAAVLLSAAWSTEAAA
                     PPEMCAAMAAVRAPVAGAADYAAARALRDRLLAEHRIEVAVSPIDDALWVRVSAHVY"
     gene            complement(1121..1534)
                     /locus_tag="VGB88_12890"
     CDS             complement(1121..1534)
                     /locus_tag="VGB88_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX9648392.1"
                     /translation="MIVYNLRCPDEHLFEAWFRDSAGFDEQVRAGAVACPTCASTDIA
                     KAPMAPNITSRRDAPAKREVPAEVLVKLKQLRAEVEHNSEHVGPRFPEEARKIHYGET
                     EPRNIHGDATADEARALSDEGIEFAVIPWVTRTDS"
     gene            1622..2161
                     /locus_tag="VGB88_12895"
     CDS             1622..2161
                     /locus_tag="VGB88_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9648393.1"
                     /translation="MSPGPGHGEGLAPSAWVSRFAPLVAAGGAVLDIASGSGRHARFF
                     LARNHPVTAVDRDLSGLADIDDARLARIEADLEGGRPWPLGPARFAAVVVANYLHRPL
                     FGPILGALAARGVLIYETFAQGNERFGRPRNPDFLLRPGELLEVARHGGLRVLAYEDL
                     EVAMPRPACIQRICAAAPG"
     gene            complement(2170..3027)
                     /locus_tag="VGB88_12900"
     CDS             complement(2170..3027)
                     /locus_tag="VGB88_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9648394.1"
                     /translation="MNESLLRVACVQMTSTPDVAANLDQAGALVREAAAGGAELVLLP
                     ENVACLAGQREMRAAAGDDGAHLARRVFADLARATGIWLLAGSIAERRRDESRLANRS
                     LLFDGRGALVATYDKIHMFDVDLDGGESYRESGNYAPGERAVLATTPWGGLGMTVCYD
                     LRFPQLYRALAKAGAAMLAIPSAFTVPTGRAHWQVLLRARAIECGCFVFAPAQCGSHY
                     GRRQTYGHSLIVDPWGEVLAEAGEAPGVIAADIDLGRVADARAKVPSLGHDRPFAAPA
                     PLETARAAE"
     gene            complement(3024..3284)
                     /gene="grxC"
                     /locus_tag="VGB88_12905"
     CDS             complement(3024..3284)
                     /gene="grxC"
                     /locus_tag="VGB88_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX9648395.1"
                     /translation="MAHVVIYTSPFCGYCHRAKRVLAGKGAEFEEVDLVQQPARRDEM
                     VERAQGRATVPQIFIDGRHVGGHEELVALERTGDLDKLLGTP"
     gene            complement(3339..4091)
                     /locus_tag="VGB88_12910"
     CDS             complement(3339..4091)
                     /locus_tag="VGB88_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9648396.1"
                     /translation="MANGALIAGAGRLLAGAFDMLMPPGCMACRGPVDAPGRLCPACW
                     AEVEFLAPPQCACCGLPFDYGIGAGATCAPCAARAPAFDRARAVMRYGAVARRLVVGL
                     KHGDRTHLVPALGAWLVRAGAELLDGADALVPVPLHRHRLLARRFNQSALLARAAARA
                     TGVPVAVDALRRTKATPSQGGLSRHQRQRNVAGAFEVAAERRASLAGRRLVLVDDVFT
                     TGATVEACARTLRRAGAGHVDVLTLARVVFAD"
     gene            4187..5098
                     /locus_tag="VGB88_12915"
     CDS             4187..5098
                     /locus_tag="VGB88_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9648397.1"
                     /translation="MDHAIEVFDRRLVRRRRARAAPGFAGFDFLVREVARRLVERLAD
                     IDRRFADALDLGCHRGEVAAALVQAGFTGTLVQCDLAPEMVALAPGGPERLRLAADEE
                     ALPFRADSFDLALSAFSLHWVNDLPGTLIQISRLLRADGLMLAAIPGAATLAGLRAAL
                     AEGELAEAGGASPRVSPFLDIRDAGALLQRAGFALPVVDTETITVAYRDPMRLLADLR
                     GMGEGNALIKRRRAPLARTTLARALENLRLSRAGADGGLEVGFEVLTLTGWKAHPGQP
                     QPLRRGSAAARLADALERLEKPGSGRE"
     gene            complement(5084..6022)
                     /locus_tag="VGB88_12920"
     CDS             complement(5084..6022)
                     /locus_tag="VGB88_12920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX9648398.1"
                     /translation="MIFAAIETDRLYLAPLEPADAPALAALLGDDTLARHTPGLPRPY
                     DHDAALGWIARSNSRLYAGEEFLVGCRLRAGGGLVGVVELALRPDEAAAELGYWFDPA
                     FWGRGLATEAVRRMVAFGFDTLGLGELRAATLVGNPASARVLAKAGLIEVAEGEVEGH
                     AARYHRLEAGRYRPQSLAPRLVYVAAAALIDGGGRVLLARRPPGKAMAGLWEFPGGKV
                     EPGERPRATLARELSEELSVEVAPSELVAFTAVCHRYDDFQLLMPLMVCRRWGGAPTP
                     REGQEVAWTTARHLTNYPMPPADAPLVRELAPLLAP"
     gene            complement(6019..7263)
                     /gene="argJ"
                     /locus_tag="VGB88_12925"
     CDS             complement(6019..7263)
                     /gene="argJ"
                     /locus_tag="VGB88_12925"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX9648399.1"
                     /translation="MAAVLPKPSPLAPAAAPELPPIAGARLAATASGIRYRGRTDLML
                     ASFDAGTAVAGMFTRSLTASPAVAWCRSALAGGQARALVVNSGNANAFTGAAGRSAVE
                     RVADATAQLIGCRREQVFSSSTGVIGEPLPVDKIIAALPYLSERLSPAGWQVAAEAIM
                     TTDTFAKAATRACDIDGRAVRINGIAKGSGMIAPDMATMLAYLATDAALPAPLLQQML
                     AASVAKSFNAITVDSDTSTSDTVLMFATGKVPVAPVPATARDRRLGAFRAALDEVCLD
                     LAHQIVRDGEGATKFVTVTVGGAASARAARRIAFAIANSPLVKTAIAGSDPNWGRIVM
                     AVGKAGEKADADALKVWIGPTQITEGGRLRAGYDEAPVAAYMKGDEITIRVDVGVGRG
                     RATVWTCDLNHGYIDINADYRS"
     gene            complement(7283..8167)
                     /locus_tag="VGB88_12930"
     CDS             complement(7283..8167)
                     /locus_tag="VGB88_12930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9648400.1"
                     /translation="MGAIARAGLVGGLLAAAWAVPALAQSPAEAATTDPAAVADDVVV
                     ARVGAEAIMLSEVIGQIYAMSEEERGKYAFDEIYRETLQRRIDRELVYLAARDNGIGE
                     DPDYLARMRILERRVMSDTYMQRELARRVSEQEIQGRYDRMAEAQVDRTELWARHIMA
                     GDEAAARALKSQLAAGADFAEVARGLDYPGASVGGDLGYFTEDNMAPEIVAVARTLEV
                     GAVSEPFETPYGWLLIKLEGERPVALRAFAEVRQQLYEELSRDAITTVLDELRAATPV
                     ARFNRDGTPLEEPAAETD"
     gene            8356..>9277
                     /locus_tag="VGB88_12935"
     CDS             8356..>9277
                     /locus_tag="VGB88_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9648401.1"
                     /translation="MISALARRIFGTPNDRLIKAHGRVVEQINALEPEIEALSDDALR
                     ARTEAFRERVAGGEALDGLLVEAFATVREAAKRTLGQRHFDVQLIGGMVLHDGKIAEM
                     KTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLARRDAEWMGQIYRFLGLEVGCILSG
                     LNTQERRRAYAADVTYGTNNEFGFDYLRDNMKFALEEYVQRDLHYALVAEVDNILNDE
                     ARTPLIISGPAEESTQKYYSIDRVIPKLRKEADYTIDEKLRQAMLTDEGVAKVERILA
                     IPNLYDPTEIDTLHHVQQALRAHTLHKKDVD"
BASE COUNT         1252 a         3462 c         3383 g         1180 t
ORIGIN      
        1 ttgtagacat gcgccgagac gcgcacccac agcgcatcgt cgatcgggct gaccgcgacc
       61 tcgatgcggt gctcggcgag caggcggtcg cgcagcgcgc gcgccgcggc gtagtcggcg
      121 gcgccggcga ccggcgcgcg caccgccgcc atcgccgcgc acatctccgg cggcgccgcg
      181 gcctcggtgc tccaggcggc gctcagcagc accgcggcct ggcgcgcgag gccggccata
      241 ttggcgcgcg ccgcttccgg gccgagctcg cgccagaagg cgagcgccgc cggcaccgcc
      301 aggacggccg agtagtcgcg ggtgccggtc cactcgaact cggtcgggaa gccggcgccg
      361 tagaggttcg agatcaccgc cgggtgcagg cgctcccggc ggtccttgcg cgcccacagg
      421 aagcccgcgc ccttgggcgc gcacagccac ttgtggcagt tgccgacata ccagtcggcg
      481 ccgatcgcgg gcacatcgag gtcgagcatg cccggggcgt gggcgccgtc gaccaggacg
      541 gcgacgccgc gctcgcggca cgcccggcac agggcggcga tcggcagcac cgtgcccgag
      601 gccgagacga cgtggtcgag caccgcgagc cgcgcacccc ggtcgagcgc cgcgaccacc
      661 ggctcgaccg cggcctcggg cccggcgagc ggcgtcggca gggccaccac gtgcagggtc
      721 gcgccgcgct cccggcacag atgggaaagc gtgttgcgca ccgccggata ggcgcccgag
      781 gtggcgagca ccacgtcgcc cgggccgagg tcgacggcgc gcagcaccgc gttcgcaccc
      841 tcggtggcgt tggcgaccag cgccaggtcg cccgcctctg cgcccaggaa cgccgccagc
      901 tccgccgccg cctgcctcag cgccggagcc aggcggtcga gcatgaacgc gaccggctgc
      961 gcctcgagct cggcgcgcca ccggtcctgg gcggcctgca ccgcccgcgg cgtggcgccg
     1021 aaggagccgt ggttgaggaa gtcgacgcgc ccgtccagcg tccacagccc ccgcgccgcg
     1081 gcgccgaagc gcggcgcgcc ggcggcgggc gcggcgccgc tcagctgtcg gtgcgcgtca
     1141 cccaggggat gaccgcgaat tcgatgccct cgtcggacag cgcgcgggcc tcgtccgcgg
     1201 tcgcgtcgcc gtggatgttg cggggctcgg tctcgccata gtggatcttg cgcgcctcct
     1261 cggggaagcg cggcccgaca tgctcgctgt tgtgctcgac ctcggcgcgc agctgcttca
     1321 gcttgaccag cacctcggcc ggcacctcgc gcttggccgg ggcgtcgcgg cggcttgtga
     1381 tattgggcgc catcggcgcc ttggcgatgt cggtgctggc gcaggtgggg caggcgaccg
     1441 cgcccgcgcg cacctgctcg tcgaagccgg cgctgtcgcg gaaccaggcc tcgaacagat
     1501 gctcgtccgg gcatctgagg ttgtacacga tcattgccgc caagcccttg aaaaacccga
     1561 caccctcggg cttcagtacc aattagatgg catgctttaa ccgccataac aagcgccggc
     1621 catgagcccc ggccccggtc acggagaggg gctggcgccg tcggcgtggg tgtcgcgctt
     1681 cgcgcccctg gtggcggccg gcggggcggt gctcgacatc gccagcggca gcggccgcca
     1741 cgcgcggttc tttcttgccc gcaaccaccc ggtgaccgcc gtcgaccgcg acctgtcggg
     1801 cctggccgac atcgacgacg cgcggctggc gcgcatcgag gccgacctcg agggcggccg
     1861 gccgtggccg ctcgggccgg ccaggttcgc cgccgtggtg gtggccaact acctgcaccg
     1921 gccgctgttc gggcccatcc tgggcgcgct ggcggcgcgc ggggtgctga tctacgagac
     1981 cttcgcccaa ggcaacgagc gtttcggccg gccgcgcaat cccgatttcc tgctccgccc
     2041 cggagagctc ctggaggtcg cgcgccacgg cggcctgcgc gtgctggcct atgaggacct
     2101 cgaggtggct atgccgcgcc cggcctgcat ccagcgcatc tgcgcggccg cgccgggctg
     2161 agggcggcgc tactcggcgg cgcgcgcggt ttcgagcggc gccggcgcgg cgaacgggcg
     2221 gtcgtggccg aggctcggca ccttggcgcg cgcgtcggcg acccggccga ggtcgatgtc
     2281 ggcggcgatc accccgggcg cctcgccggc ctcggccagc acctcgcccc acgggtcgac
     2341 gattaggctg tggccgtagg tctgccgccg gccgtaatgg ctgccgcact gggccggcgc
     2401 gaacacgaaa cagccgcact cgatcgcgcg cgcgcgcagc agcacctgcc agtgcgcacg
     2461 cccggtcggc acggtgaacg ccgaggggat cgccagcatc gccgcgccgg ccttggccag
     2521 cgcccggtag agctgcggga agcgcaggtc gtagcacacg gtcatgccga gcccgcccca
     2581 cggcgtcgtc gccagcacgg cgcgctcgcc cggggcgtag ttgcccgact cgcggtagct
     2641 ctcgccgccg tcgaggtcga cgtcgaacat gtgaatcttg tcgtaagtcg cgaccagcgc
     2701 gccgcggccg tcgaacagca gcgagcggtt ggcgagccgc gactcgtcgc gacggcgctc
     2761 ggcgatgctg ccggcgagca gccagatgcc ggtggcgcgg gcgagatcgg cgaatacgcg
     2821 gcgcgcgagg tgcgcaccgt catcgccggc ggcggcgcgc atctcgcgct gcccggccag
     2881 gcaggcgacg ttctcgggca gcagtaccag ctcggcgccg ccggccgccg cctcgcgcac
     2941 caatgcgccc gcctggtcga ggttcgcggc cacgtccggc gtgctggtca tctgcacgca
     3001 ggcgacgcgc agcaaggact cgttcatggc gtccccagca gcttgtcgag gtcgcccgtg
     3061 cgctcgagcg cgaccagctc ctcgtggccg ccgacgtggc ggccgtcgat gaagatctgc
     3121 ggcacggtcg cgcgaccctg ggcgcgctcg accatctcgt cgcgccgcgc cggttgctgg
     3181 accaggtcga cctcctcgaa ctcggcgccc ttgccggcca gcacccgctt ggcgcggtgg
     3241 cagtagccgc agaacggcga ggtgtagatc acgacatggg ccatggcgga ctcgccgcgg
     3301 ttgcgcgcgc ccggcgcgct tgcctcaagc ctagcaagct agtcggcgaa caccacccgg
     3361 gcaagggtga gcacgtcgac gtgaccggcg ccggcgcgcc tgagggtgcg cgcgcaggcc
     3421 tcgacggtgg cgccggtggt gaacacgtcg tcgaccagca ccagccggcg gccggcgagg
     3481 gacgcccgcc gctccgccgc cacctcgaac gcgccggcga cgttgcgctg gcgctggtgg
     3541 cggctgagcc cgccctggct cggcgtcgcc ttggtccggc gcagcgcgtc caccgccacc
     3601 ggcacaccgg tcgcccgcgc cgcggcccgg gcgagcagcg ccgactggtt gaagcggcgg
     3661 gcaagcaggc ggtggcggtg cagcggcacc ggcaccagcg catcggcgcc gtcgagcagc
     3721 tcggcccccg cccgcacgag ccacgcgccg agcgccggca ccagatgggt gcggtcgccg
     3781 tgcttgaggc cgaccaccag ccggcgcgcg accgcgccgt agcgcatcac cgcccgcgcc
     3841 cggtcaaagg cgggggcgcg cgccgcgcag ggcgcgcagg tcgcgcccgc gccgatgccg
     3901 taatcgaacg gcagcccgca gcacgcgcac tgcggcgggg ccaggaactc gacctcagcc
     3961 cagcaagccg ggcacagccg cccgggcgcg tccaccggcc cgcggcacgc catgcagccc
     4021 ggcggcatca gcatgtcgaa cgcgccagcc agaagtcggc ccgcgcctgc gatcaacgcg
     4081 ccgttcgcca tcccgcgccc ccgacccgat cgagatcgcc ccgccccacc cgccggcccg
     4141 gtggacaggc gggccgggcg gcgggttatc tgataatagc agagcgatgg accacgcgat
     4201 cgaggtgttc gaccggcgcc tggtgcgccg gcgccgcgcg cgtgccgctc cgggtttcgc
     4261 cgggttcgac ttcctggtgc gcgaggtcgc ccggcgcctg gtcgagcggc tggccgacat
     4321 cgatcggcgg ttcgccgacg cgctcgacct cggctgccac cgcggcgagg tcgcggcggc
     4381 gctggtgcaa gccggcttca ccggcacgtt ggtccagtgc gacctggcgc cggagatggt
     4441 cgcgctggcg ccgggcgggc cggagcggct gcgcctcgcc gccgacgagg aggcgctgcc
     4501 gttccgcgcc gacagcttcg acctggcgct gagcgcgttc agcctgcatt gggtcaacga
     4561 tcttcccggc accctgatcc agatttcgcg gctgttgcgc gcggacgggc tgatgctggc
     4621 cgcgattccg ggcgcggcga cgctcgccgg gctgcgcgcc gcgctcgccg agggcgagct
     4681 cgccgaggcg ggcggcgcca gcccgcgcgt ctcccccttc ctcgacatcc gcgatgccgg
     4741 cgcgctgttg cagcgcgcgg gcttcgcgct gccggtggtc gataccgaga ccatcacggt
     4801 ggcgtaccgg gacccgatgc gcctcctcgc cgacctgcgc ggcatgggcg agggcaacgc
     4861 gctgatcaag cgccggcgcg cgccgctcgc ccggacgacg ctcgcccgtg cgctcgagaa
     4921 cctgcgcctc agccgcgccg gcgccgacgg cggcctcgag gtcggcttcg aggtgctgac
     4981 cttgaccggc tggaaggcgc atcccggcca gccgcagccg ctcaggcgag gcagcgccgc
     5041 ggcgaggctc gccgatgcct tggaacggct cgaaaagccc gggtcagggc gcgagtagcg
     5101 gcgccagctc ccggaccagc ggagcgtcgg ccggcggcat cgggtaattg gttaggtggc
     5161 gagccgttgt ccaggcaact tcctggccct cgcgcggcgt cggtgccccg ccccagcgcc
     5221 ggcagaccat cagcggcatc agcagctgga aatcgtcgta gcggtggcag accgcggtga
     5281 acgccaccag ctcggacggc gccacctcga ccgacagctc ctcggacagc tcgcgggcaa
     5341 gcgtggcgcg cggccgctcg ccgggctcga ccttgccgcc gggaaactcc cacagcccgg
     5401 ccatcgcctt gcccggcggc cggcgcgcga gcagcacgcg cccgccaccg tcgatcagcg
     5461 ccgccgcggc gacgtagacc aggcgcggcg cgagcgactg cgggcggtag cggccggcct
     5521 cgaggcgatg gtagcgcgcg gcatggccct cgacctcgcc ctcggcgacc tcgatcaggc
     5581 cggccttggc cagcacccgg gcgcttgccg ggttgccgac cagggtcgcc gcgcgcagct
     5641 cgccgaggcc gagggtgtcg aagccgaagg cgaccatccg gcgcaccgcc tcggtggcga
     5701 ggccccgccc ccagaaggcg ggatcgaacc agtagccgag ctcggccgcc gcctcgtcgg
     5761 ggcgcagcgc cagctcgacc accccgacca gcccgccgcc ggcgcgcagc cggcagccga
     5821 ccaggaactc ctcgccggca tagaggcggg agttggagcg cgcgatccag cccagcgccg
     5881 cgtcgtggtc gtaaggccgc ggcaggcccg gcgtgtggcg ggccagcgtg tcgtcgccga
     5941 gcagcgcggc gagcgcgggc gcgtcggcgg gctcgagcgg agcgaggtaa agccgatcgg
     6001 tctcgatcgc ggcgaaaatc acgaccggta gtcggcgttg atgtcgatgt agccgtggtt
     6061 gaggtcgcag gtccacacgg tcgcccggcc gcggccgacg ccgacatcga cgcgaatcgt
     6121 gatctcgtca cccttcatgt aggcggcgac cggcgcctcg tcgtagccgg cgcgcagcct
     6181 gccgccctcg gtgatctggg tcggcccgat ccagaccttg agcgcgtccg cgtcggcctt
     6241 ctcgccggcc ttgccgaccg ccatcacgat ccgcccccag ttgggatccg agcccgcgat
     6301 cgcggtcttg accagcggcg agttggcgat cgcgaacgcg atccgccgcg ccgcgcgcgc
     6361 gctcgcggcg ccgccgaccg tgaccgtgac gaacttggtc gcgccctcgc cgtcgcgcac
     6421 gatctggtgg gcgaggtcga ggcacacctc gtccagcgcg gcgcgaaagg cgccgaggcg
     6481 gcggtcgcgc gccgtggccg gcacgggcgc aaccggcacc ttgccggtgg cgaacatcag
     6541 caccgtgtcg ctggtcgagg tgtcgctgtc gaccgtaatc gcattgaacg acttggccac
     6601 cgacgcggcg agcatctgtt gcagcagcgg cgccggcagc gcggcgtcgg tggcgaggta
     6661 ggcgagcatg gtcgccatgt cgggcgcgat catgcccgag cccttggcga tgccgttgat
     6721 gcgcaccgcc cggccgtcga tgtcgcaggc gcgggtggcg gccttggcga aggtgtcggt
     6781 ggtcatgatc gcctccgcgg ccacctgcca gcccgcgggc gacagccgct cgctcaagta
     6841 gggcagcgcg gcgatgatct tgtcgaccgg cagcggctcg ccgatcacgc cggtcgatga
     6901 gctgaacacc tgctcgcgcc ggcagccgat cagctgggcg gtggcgtccg cgacccgctc
     6961 gaccgccgag cggccggcgg cgccggtgaa cgcgttggcg ttgcccgagt tgaccaccag
     7021 cgcgcgcgcc tgcccgccgg cgagcgcgct ccggcaccag gcgaccgcgg gcgaggcggt
     7081 gagcgagcgg gtgaacatcc cggcgaccgc ggtgccggca tcgaagctcg ccagcatcag
     7141 gtcggtgcgc ccccggtagc ggatgcccga ggcggtggcg gccaggcgcg cgccggcgat
     7201 cggcggcagc tcgggcgcgg ccgcgggcgc aagcggtgag ggtttcggca ggaccgccgc
     7261 catcgacgaa tcgaccgccg gttcagtcgg tctcggccgc cggttcctcg agcggcgtgc
     7321 cgtcgcggtt gaagcgcgcg accggggtcg ccgcccgcag ctcgtcgagc acggtggtga
     7381 tggcgtcgcg cgacagctcc tcgtagagct gctggcgtac ctcggcaaag gcacgcagcg
     7441 cgaccgggcg ctcgccctcg agcttgatca gcagccagcc ataaggggtc tcgaacggct
     7501 ccgagacggc gccgacctcg agcgtgcgcg ccaccgccac gatctcgggc gccatgttgt
     7561 cctcggtgaa gtagccgagg tcgccgccca ccgaggcgcc cggatagtcg agcccgcgcg
     7621 cgacctcggc gaagtcggcg ccggcggcga gctgcgactt cagcgcccgc gccgcggcct
     7681 cgtccccggc catgatatgg cgcgcccaca gctcggtgcg atcgacctgc gcctcggcca
     7741 tccggtcgta gcggccctgg atctcctgct cgctcacccg gcgggcgagc tcgcgctgca
     7801 tgtaggtgtc cgacatcacc cggcgctcga ggatgcgcat ccgcgccagg tagtcggggt
     7861 cctcgccgat gccgttgtcg cgggccgcca ggtagaccag ctcgcggtcg atccggcgct
     7921 gcagggtctc gcggtagatc tcgtcgaagg cgtacttgcc gcgctcctcc tcggacatgg
     7981 cgtagatctg gccgatgacc tcggacagca tgatggcctc ggcgccgacc cgggccacca
     8041 ccacgtcgtc ggcgaccgcg gccgggtcgg tcgtcgccgc ctccgcgggc gactgcgcca
     8101 acgcgggcac ggcccacgcc gcggcaagca gcccgccgac caggccagcg cgcgcgatcg
     8161 ctcccatcgt gatacccttt ctcgtgccgg acccccgccg cacggcacgc gccggggtca
     8221 gccggcatca tgcatattgc ccgcaccgcg acaagcggct cggggcgggc gcgttgacag
     8281 gctcatggtg gacacttatc tgaaggctcg aaccgcggac gcccggcgtc cgcccccgcc
     8341 tcacgggtcc gcttcatgat cagcgcgctc gcccgccgca tattcggcac gccgaatgac
     8401 cgcctgatca aggcgcatgg gcgcgtggtc gagcagatca acgccctcga gcccgagatc
     8461 gaggcgctgt cggacgacgc gctgcgcgcg cgcaccgaag cgttccgcga gcgcgtggcg
     8521 ggcggcgagg cgctcgatgg gctcctggtc gaggcgttcg cgaccgtgcg cgaggcggcc
     8581 aagcgcaccc tgggccagcg ccatttcgac gtccagctga tcggcggcat ggtgctgcac
     8641 gacggcaaga tcgccgagat gaagaccggc gagggcaaga ccctggtggc gacgctcgcg
     8701 gtctacctga acgcgcttgc cggcaagggc gtgcacgtgg tcacggtcaa cgactacctc
     8761 gcccgccgcg acgccgagtg gatgggccag atctaccgct tccttggcct cgaggtcggc
     8821 tgcatcctga gcggcctcaa cacccaggag cgccgccgcg cctacgccgc cgacgtcacc
     8881 tacggcacca acaacgagtt cggcttcgac tacctgcgcg acaacatgaa gttcgcgctc
     8941 gaggagtacg tccagcgcga cctccactac gcgctcgtcg ccgaggtcga caacatcctg
     9001 aacgacgagg cgcgcacgcc gctcatcatc tccgggcccg cggaggagtc cacgcagaag
     9061 tattactcga tcgatcgggt gattccgaag ctccgcaagg aggccgacta cacgatcgac
     9121 gaaaagctgc gtcaggccat gctcaccgac gaaggcgtgg ccaaggtgga gcgcatcctc
     9181 gccatcccga acctctacga cccgaccgag atcgacaccc ttcaccacgt gcagcaggcg
     9241 cttcgcgccc acacgctgca caagaaggac gtcgact
//
LOCUS       DASWHU010000347         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2083749, whole genome shotgun sequence.
ACCESSION   DASWHU010000347 DASWHU010000000
VERSION     DASWHU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083749"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGB88_12940"
     CDS             <1..273
                     /locus_tag="VGB88_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648402.1"
                     /translation="APDFAEGWNKRATLYWLMGDFEASVADIERTLALEPRHFGALSG
                     LAMIRMAQQRLADAAKALKAVIAIHPHAGDAARQLERIEQIIGTET"
     gene            complement(286..1755)
                     /gene="pyk"
                     /locus_tag="VGB88_12945"
     CDS             complement(286..1755)
                     /gene="pyk"
                     /locus_tag="VGB88_12945"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9648403.1"
                     /translation="MNARSRKAKILATLGPATASRAALEALHGAGADAFRLNFSHGSH
                     HDIAALYRTIREVEEQARHPIAVVADLQGPKLRVGHLKGGAVTLAAGQPFRLDLTRAK
                     GDEARAPLPHPEVFAALAPGMQLLLDDGRIRLKVTRCGPRFADTEVVTGGRLSDHKGV
                     NIPGAVLAIKALTEKDRRDLAFAVNLGVDWIALSFVQRPDDIAEARRLAGPGTAIMAK
                     IEKPAAVERFAEILELADGIMVARGDLGVEMAPEEVPRIQKQIVRAAREAGKPVVVAT
                     QMLDSMVHAPEPTRAEASDVATAVYDGGDALMLSAETAVGEYPERAVDMMRRIIERVE
                     DDPLHRQMLDAAHANPTPTAADAISAAAHQVADTVGARAIITYTQSGATALRAARERA
                     RMPVIALTPNRGMARRLALVWGLHCIFGDDARDFDDMVARACRAAHFRGYAEAGERVV
                     IIAGVPFGTPGATNLLHIAYIPPFAERRKTPRPLIAAAE"
     gene            complement(1752..3047)
                     /locus_tag="VGB88_12950"
     CDS             complement(1752..3047)
                     /locus_tag="VGB88_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9648404.1"
                     /translation="MTGTPKSVLRAMFDAAVASALPERVVPAHLPPAPAGRCVVVGAG
                     KAAGAMARAVEAAWADAGRKGALDGLVITRYGHKVETLRIRVVEAAHPVPDRAGLAAA
                     AEILGLVSGLGANDQVLCLISGGGSALLTLPAAGLDLDDKRAVTAELLRCGATISEIN
                     CVRKHLSAIKGGRLAAAAHPAEVVTLMISDVPGDDPAVIASGPTVADPTTFADARAVL
                     AKYAITPPAAVAKRLAAVADETPKPGDARLARARAILIATPQSALEAAAGVARGVGYA
                     PLILSDSIEGEARDVALVHAAIARQVALRGQPARAPCALISGGETTVTVRGQGRGGRN
                     AEFLLALTIALDGLGHVHAIACDTDGVDGSEDNAGALCGPDSLARAAAAGVDARARLA
                     DNDAYGFFAALGDLVVSGPTLTNVNDFRAILVADRVGER"
     gene            complement(3098..>3994)
                     /locus_tag="VGB88_12955"
     CDS             complement(3098..>3994)
                     /locus_tag="VGB88_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9648405.1"
                     /translation="TRLAVVLLLLGTFAIGFSPTFVRLSEIGPVATAVWRVALAVPAL
                     FAVVAVEGAPRRTPTGAEGLTRADVGGLVLAGLLFAGDLAFWHWSIAATSVANAALFA
                     TSTPVFVTLAAWAWFGEHITRRFLIGLALSLAGAAMLAGASLRSAPGHLLGDLFGIVT
                     ALFFSGYLLTVKRLRARLSAARIMAWSGLVTAAALAALNTIVGEAWAPISAAGWLVLV
                     GLALLSHAGGQGLVAQALATLPASFSAVAMLSEPVFAGAIAWTVLSEAVGPLQAAGGA
                     VILAGIFLAMPRRRALQAAAVV"
BASE COUNT          558 a         1476 c         1474 g          486 t
ORIGIN      
        1 gcgcccgact tcgccgaagg ctggaacaag cgcgcgacct tgtactggct gatgggcgac
       61 ttcgaggcct cggtcgccga catcgagcgc acgctcgcgc tcgagccgcg ccacttcggc
      121 gcgctgtcgg ggctcgccat gatccgcatg gcccagcaac ggctcgcgga cgcggccaag
      181 gcgctcaagg cggtgatcgc gatccacccc cacgccggcg acgccgcccg ccaactcgag
      241 cgcatcgagc agatcatcgg caccgagacc tgataccggc gccgctcact ccgccgcggc
      301 gatcagcggg cgcggcgtct tgcggcgctc ggcgaacggc gggatgtagg cgatgtgcaa
      361 gaggttggtc gcgccggggg tgccgaacgg gacgccggcg atgatcacca cccgctcgcc
      421 ggcctcggcg tagccgcgga agtgcgcggc gcggcaggcg cgcgcgacca tgtcgtcgaa
      481 gtcgcgcgcg tcgtcgccga agatgcagtg caacccccac accagcgcca gccggcgcgc
      541 catgccgcgg ttgggggtga gtgcgatcac cggcatccgg gcacgctcgc gcgcggcgcg
      601 cagcgcggtc gcgcccgact gggtgtaggt gatgatggcg cgcgcgccga ccgtgtcggc
      661 gacttggtgc gcggcggcgc tgatcgcgtc ggccgcggtc ggggtggggt tggcgtgggc
      721 ggcatcgagc atctggcggt gcagcgggtc gtcctccacc cgctcgatga tccggcgcat
      781 catgtcgacc gcgcgctcgg ggtactcgcc gaccgcggtc tcggccgaca gcatcagcgc
      841 atcgcccccg tcatagaccg cggtggcgac gtcggaggcc tcggcgcggg tgggctcggg
      901 cgcgtgcacc atcgaatcga gcatctgcgt cgccaccacc acgggcttgc ccgcctcgcg
      961 cgccgcgcgc acgatctgct tctggatgcg cggcacctcc tcgggcgcca tctcgacgcc
     1021 gaggtcgccg cgcgcgacca tgatgccgtc ggcgagctcg aggatctcgg cgaagcgctc
     1081 gaccgccgcc ggcttctcga tcttggccat gatcgcagtg ccggggccgg cgagcctgcg
     1141 tgcctcggca atgtcgtcgg gacgctggac gaagctgagc gcgatccagt cgacgccgag
     1201 gttgaccgcg aacgcgaggt cgcgccggtc cttctccgtc agcgccttga tcgccagcac
     1261 cgcgcccgga atattcaccc ccttgtggtc gctgagcctg ccgccggtca ccacctcggt
     1321 gtcggcgaag cgcgggccgc atctggtcac cttgagccgg atgcggccgt cgtcgagcag
     1381 cagctgcatg cccggggcga gggcggcgaa cacctccggg tgcggcagcg gcgcgcgcgc
     1441 ctcgtcgccc ttggcgcggg tgaggtcgag gcggaacggc tgcccggcgg ccaaggtcac
     1501 cgcgccgccc ttgagatggc cgacgcgcag cttcggccct tgcaggtcgg cgaccaccgc
     1561 gatcgggtgg cgcgcctgct cctcgacctc gcggatggtg cggtagagcg cggcgatgtc
     1621 gtgatggctg ccgtggctga agttaagccg gaaggcatcg gcgccggcac cgtggagtgc
     1681 ctccaatgcg gcgcggctgg cggtcgccgg gccgagggtg gcgaggatct tggccttgcg
     1741 cgagcgcgcg ttcatctctc gcccacccgg tccgcgacca ggatcgcgcg gaaatcgttg
     1801 acgttggtca gggtcgggcc gctcaccacc aggtcaccaa gcgccgcgaa gaagccgtag
     1861 gcgtcgttgt cggcgagccg cgcgcgcgca tcgacgcccg ccgccgcggc gcgggccaag
     1921 ctgtccgggc cgcacagcgc gccggcgttg tcctcgctgc cgtcgacgcc gtcggtgtcg
     1981 caggcgatgg cgtggacgtg gccgaggccg tcgagcgcaa tcgtgagcgc gagcaggaac
     2041 tcggcgttgc gcccgccgcg cccctgcccg cgcaccgtta ccgtggtctc gccgcccgag
     2101 atcagcgcgc agggcgcgcg cgccggctgg ccgcgcagcg ccacctggcg cgcgatcgcg
     2161 gcgtggacca gggcgacgtc gcgcgcctcg ccctcgatcg aatcgctcag gatcaaaggc
     2221 gcgtagccga cgccgcgggc gactccggcg gcggcctcga gcgccgactg cggggtcgcg
     2281 atcaagatag cgcgcgcgcg cgcgagccgc gcatcgcccg gcttcggcgt ctcgtccgcc
     2341 accgcggcga ggcgcttcgc caccgccgcc ggcggcgtga tcgcatactt cgccagcacc
     2401 gcgcgcgcat cggcgaaggt ggtcgggtcg gccacggtcg ggcccgaggc gatcaccgcc
     2461 gggtcatcgc ccgggacgtc cgagatcatc aaggtcacca cctcggccgg atgggcggcg
     2521 gcagccagcc ggccgccttt gatggccgac aaatgcttgc gcacgcaatt gatctcggag
     2581 atggtcgcgc cgcagcgcaa gagctcggcc gtcaccgcgc gcttgtcgtc gaggtcgagc
     2641 cccgccgccg gcagggtcag caacgccgag ccgccgccgg agatcaggca cagcacctgg
     2701 tcgtttgcgc cgaggccgga gacgagcccc aggatctccg ccgccgccgc gagcccggcg
     2761 cggtcgggca ccgggtgcgc ggcctccacc acccggatgc gcagggtctc gaccttgtgg
     2821 ccgtagcggg tgatcaccaa gccgtcgagc gcgcccttgc gccctgcgtc cgcccacgcc
     2881 gcctcgaccg cgcgcgccat cgcgccggcg gccttgccgg cgccgacgac gacgcagcgc
     2941 ccggccggcg ccggcggcag gtgcgccggc accacgcgct cgggcaaggc cgaggcgacg
     3001 gcggcgtcga acatcgcgcg cagcaccgat ttcggcgtgc cggtcatgcc cgctccccga
     3061 tcaggctcgg tcgtaagcct aacggattcg tggcgcatca aaccaccgcc gcggcctgga
     3121 gcgcccgccg gcggggcatc gccaggaaga tgccggccag gatgacggcg ccgccggcgg
     3181 cctgcagcgg cccgaccgcc tcgctcagca ccgtccaggc gatggcgccg gcgaacaccg
     3241 gctcgctcaa catcgcgacc gccgagaacg acgccggcag ggtcgccagc gcctgggcga
     3301 ccaggccctg gccgccggcg tggctgagca gcgcgaggcc gaccagcacc agccagccgg
     3361 cggcgctgat gggcgcccac gcctcgccga caatcgtgtt gagcgccgcc agcgccgccg
     3421 ccgtcaccag tcccgaccag gccatgatcc gcgccgccga cagccgcgcg cgcaggcgct
     3481 tgacggtcag cagatagccc gagaagaaca gcgccgtgac gatcccgaac aggtcgccga
     3541 gcaggtgtcc gggcgcgctg cgcaacgacg cgccggcgag catcgccgcg ccggcgaggc
     3601 tgagcgcgag gccgatcagg aaccgccgtg tgatgtgctc gccgaaccat gcccaggcgg
     3661 cgagcgtgac gaacaccggc gtcgaggtcg cgaacagggc ggcgttggcg accgaggtgg
     3721 cggcgatcga ccagtgccag aaggcgaggt cgccggcgaa cagcagtccc gccagcacca
     3781 gcccgccgac atcggcgcgg gtgagacctt cggcgccggt cggcgtccgg cgcggggctc
     3841 cctcgacggc gaccaccgcg aacagcgccg gcacggcgag cgccacgcgc cagaccgcgg
     3901 tcgccaccgg gccgatctcg ctcaggcgca cgaaggtcgg cgagaagccg atcgcgaagg
     3961 tgccgagcaa cagcaggacg acggcaagac gtgt
//
LOCUS       DASWHU010000348         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2812387, whole genome shotgun sequence.
ACCESSION   DASWHU010000348 DASWHU010000000
VERSION     DASWHU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812387"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGB88_12960"
     CDS             <1..240
                     /locus_tag="VGB88_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9648406.1"
                     /translation="VQEPLPPLAAGDLVAFRSAGAYGAVMASTYNSRPLVAEVLVSGD
                     RFAVVRPRMDYATLIGRDRLPEWLSERAPLSRGAA"
     gene            270..2747
                     /locus_tag="VGB88_12965"
     CDS             270..2747
                     /locus_tag="VGB88_12965"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEX9648407.1"
                     /translation="MASSAEDDIGQVAGLPRSRIACQLALSAAALLWERGWPAMWPAV
                     GVAGLFVAIGLFGIWGVVPWWLHLGLLAGFAGALAWSLWRARGALRVPRRIETIRRLE
                     RTSGIAHRPLSTLLDEPATAGGPESRALWQQHQGRAAKLVRLLRVGWPAAGLAQRDPH
                     GLRAALVLLLVIGFATAGDQAGTRIAQALEPDFAGLAGARWGRLTAWIDPPDYTRLAP
                     VFLSGSSQGEPVAAGAEAQAVPAGAIEVVGGSVLAARISGGRSVPVLKRDHGEARFEA
                     VGDDNYTIEVAVEGGGALEIVQGRHSFGRWQLAVRADTAPTVALPSAPDQTTRGVLHL
                     AYQAEDDFGLAQVNARIHRRDQGGDVAPIELALPLPGLEPLSVRDSTYRDLTPHPWAG
                     LPVILELSALDNAGQTGRAPEFPMVLPERPFDHPVAKAIVEQRRRLALDRTTRVDVRQ
                     RIRMIGEAPEAFGDDVVAYLGLRVVYERLRRPQDEASEQGVLDLMWDLALRIEDGTLS
                     IAERELRAAQQALQDALEGDATPEELSALMDQLQAALEKYLEALSESGKDLADQRDDR
                     QYAESEMVQRSAQDFRRMLDQARDMAMTGAREQAQEMLRRMQEMLENLEVGRAATQPA
                     QGPAEELMKQLEQIMTDQDQLLDDTFQRAISRGENERETPGRGETGADRQEALRRALG
                     DVMRQVGEAGAEIPEGLGRAERLMRDSREELERDRADRAIDPQAQALQALQQGAQQLL
                     NRQMAGQAGERPGGRDGRQPFDENRDPLGRLPPGNTGDPRGYVEVPKQSDIERSREIL
                     NELYRRAGQPQRPQTERDYIDRLLRWY"
     gene            complement(3000..>3321)
                     /locus_tag="VGB88_12970"
     CDS             complement(3000..>3321)
                     /locus_tag="VGB88_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648408.1"
                     /translation="GLEMRNITARIEPAGDGTRLVIEGNIVNVSREVRPVPLVRVVLR
                     DDRRDLASWRFLATDVNMIPNENVSFTTFYDRVPASATGAALGFSSAPVTNVPVPERA
                     AARE"
BASE COUNT          463 a         1135 c         1286 g          437 t
ORIGIN      
        1 gtccaggagc cgttgccgcc gctggccgcg ggcgacctgg tcgcgttccg ctccgccggc
       61 gcctatggcg cggtgatggc ctcgacctac aacagccggc cgctggtggc cgaagtgctg
      121 gtgtcgggcg accgcttcgc cgtggtgcgc ccgcgcatgg attatgcgac actgatcggg
      181 cgcgatcgcc tgcccgagtg gctcagcgag cgcgcgccgc tgtcgagagg agccgcgtga
      241 tcggtcgagc atccaggggc gatgtgccgg tggcatcatc ggccgaggac gacatcggcc
      301 aggtggccgg gctgccgcgg tcgaggatcg cgtgccagct cgccttgagc gccgccgcgc
      361 tgctctggga acgcggctgg ccggcgatgt ggccggcggt gggggtggcc ggcctgttcg
      421 tcgccatcgg cctgttcggt atctggggcg tggtgccgtg gtggctgcat ctcggcctgc
      481 tggcgggttt cgccggcgcg ctcgcctggt cgctgtggcg cgcgcggggg gcgctgcggg
      541 tgccgcggcg catcgagacc atccgtcgcc tggagcggac cagcggcatc gcccaccggc
      601 cgctgtcgac gttgctcgat gagccggcga cggccggcgg gccggaatcg cgcgcgctat
      661 ggcagcagca tcaggggcgg gcggcgaagc tggtgcggct gttgcgcgtg ggctggccgg
      721 ccgccgggct ggcgcaacgc gatccccacg gcctgcgcgc ggcgctggtg ctgctgctgg
      781 tgatcggctt cgctaccgcc ggggaccagg cgggcacgcg catcgcccag gcgctcgagc
      841 ccgacttcgc cggcctggcc ggcgcccgct ggggccggct gacggcgtgg atcgatccgc
      901 ccgactacac gcgccttgcg ccggtgttcc tgtccggatc gagccaaggc gagccggtgg
      961 cggcgggcgc cgaggcgcaa gcggtaccgg cgggcgcgat cgaggtggtg ggcggcagcg
     1021 tgctggcggc gcggatcagc ggtggccggt cggtccccgt gctcaagcgc gaccacggcg
     1081 aggcgcggtt cgaggcggtc ggcgacgaca actacaccat cgaggtggcg gtcgagggcg
     1141 gcggcgcgct cgagattgtc cagggccgcc actcgttcgg ccgctggcag cttgccgtgc
     1201 gcgccgacac ggcgccgacc gtcgccctgc cctccgcgcc cgaccagacc acgcgcggcg
     1261 tgctccatct cgcctaccag gctgaggacg acttcggcct cgcccaggtc aacgcgcgaa
     1321 tccaccggcg cgaccagggc ggcgacgtgg cgccgatcga gctggcgctg ccgctgcccg
     1381 ggctcgagcc gctgagcgtg cgcgattcga cctaccgcga cctgaccccg cacccgtggg
     1441 cggggctgcc ggtgatcctc gagctgtccg ccctggacaa cgccggccag accggccggg
     1501 cgcccgagtt cccgatggtc ctgcccgagc ggccgtttga tcatccggtg gccaaggcga
     1561 tcgtcgagca gcggcggcgg ctggcgctcg acaggaccac gcgcgtcgat gtgcgccagc
     1621 gcatccgcat gatcggcgag gcgccggagg cattcggcga cgacgtcgtc gcgtatctcg
     1681 gcctgcgcgt ggtttacgag cggctgcgcc ggccgcagga cgaggcgagc gagcagggcg
     1741 tcctcgacct gatgtgggac ctggcgctgc gcatcgagga cgggaccttg tcgatcgccg
     1801 aacgcgagct gcgcgcggcc cagcaggcgt tgcaggacgc gctcgaaggg gacgcgacgc
     1861 ccgaggagct gagcgcgctg atggaccagc ttcaggcggc gctcgagaag tatctcgagg
     1921 cgttgagcga gagcggcaag gaccttgcag accaacgcga cgatcgccaa tacgccgagt
     1981 ccgagatggt gcagcggagc gcccaggact tccgccgcat gcttgaccag gcgcgcgaca
     2041 tggcgatgac cggcgcccgc gagcaggcgc aggagatgct caggcggatg caggagatgc
     2101 tcgagaacct cgaggtcgga cgcgccgcca cgcagccggc ccagggccct gcggaggagc
     2161 tgatgaagca gctcgagcag atcatgaccg accaggacca gctgctcgac gacaccttcc
     2221 agcgggcgat ctcgcgcggc gagaacgagc gcgagacgcc gggccgcggc gagaccggcg
     2281 ccgaccgcca ggaagcgctg cggcgcgcgc tcggcgacgt gatgcgtcag gtcggagagg
     2341 cgggggcgga gatcccggaa ggcctcggcc gcgccgagcg cctgatgcgc gattcgcgcg
     2401 aggagctgga gcgcgaccgt gccgatcgcg cgatcgaccc gcaggcgcag gcgctgcagg
     2461 cgctgcagca gggcgcccaa cagctgctga accggcagat ggcgggccag gcgggggaac
     2521 ggcccggcgg gcgcgacggg cgccagccgt tcgatgagaa ccgggatccg ttggggcgtc
     2581 tgccgccggg caacaccggc gatccgagag gctacgtcga ggtaccgaag caatcggata
     2641 tcgaacgcag ccgcgagatc ctgaacgagc tttatcgccg cgcgggccag ccgcagcgcc
     2701 cgcagaccga gcgcgactac atcgatcgcc tgctccgctg gtactgagac cggcgagcat
     2761 atgaacgacg ccgcccatgc cgtcatttcg gccggagcgg agcggagagc cggaatccag
     2821 ggccaccgat cgagtggtag gatccccagg cgccgtcggc gcttcgcgcc gtcctggacc
     2881 ccgggtctcg gctccgcctc gcccggggtg acgcaatggt gtcgacgggc gccggcgagt
     2941 caattggttc gctcgctggt aatgagaccg gcgcgccgac cgggggcgcc gcccgaggac
     3001 tactcgcgcg ccgcggcgcg ctcgggcacg ggcacgttgg tcacgggagc gctggagaag
     3061 ccgagcgccg cgccggtggc gctcgccggc acccggtcgt agaaggtggt gaacgagacg
     3121 ttctcgttcg ggatcatgtt gacgtcggta gccaggaacc gccagctcgc caggtcgcgg
     3181 cggtcgtcgc gcagcaccac ccgcaccagc ggcaccggcc tgacctcgcg cgagacgttg
     3241 acgatgttgc cctcgatcac cagccgcgtg ccgtcgccgg cgggctcgat gcgggcggtg
     3301 atgttgcgca tctcaaggcc c
//
LOCUS       DASWHU010000349         5579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1713106, whole genome shotgun sequence.
ACCESSION   DASWHU010000349 DASWHU010000000
VERSION     DASWHU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713106"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..525)
                     /locus_tag="VGB88_12975"
     CDS             complement(64..525)
                     /locus_tag="VGB88_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9648409.1"
                     /translation="MRRRNVLGVVVAALALVLAAPAVADITFARTTLSIEAGSGRHSF
                     DVELARTPAQTRRGLMFRGELADDAGMLFDFGEPQPIAMWMKNTLIPLDMVFVGKDGT
                     ITRIARWTTPLSLEPISSGGPVRAVLELKGGITERLGIVAGDRVVFPDPGQ"
     gene            complement(525..1595)
                     /locus_tag="VGB88_12980"
     CDS             complement(525..1595)
                     /locus_tag="VGB88_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648410.1"
                     /translation="MALVALSLGRPEGATAPGVAVTRWTLDVPVLAAAFAGNGAHAAF
                     GLDDGSVRLVALARFGGRRMTATAVHHGPVRALSADGATGGVLSLGADGRVVQLDGAG
                     AATALLDPQGAHGGARLVAGGWLRAVATAGEVIVYDRAGHVGWRAGCDGASALAASPS
                     GDTVAAARGREVTVWRDRPRETPAAALSASAPVHALAFSPYGRRLAAATDSPTALVWD
                     LGDAAGTGALFGSARDLAWSACGRALVGAGDGALLCWRTDRRVHLRGWHVPMAVAPMA
                     GCAVTALACHPRHPLAAAGYDDGSVLLADLDHGRDRVIRHGAEDAVSALAWSPQGSHL
                     ACATAGGEAALFDFTTLVKRPH"
     gene            complement(1601..2593)
                     /locus_tag="VGB88_12985"
     CDS             complement(1601..2593)
                     /locus_tag="VGB88_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9648411.1"
                     /translation="MTGRPDGDPRCPVTLVTGFLGSGKTTLLAALLARPALADSAVVI
                     NEFGEVAIDDALVGGRAGEVVRLESGCICCTARGGLEHALVDLARRRDAGTVPGFARV
                     LVETTGLADPVPVVRALHATAALAKRYRLAAVVVTVDAVTGFRRLDDAPEALRQVALA
                     DRILVTKTDLASADAGARLEARLAAVNPGAARHRVVKGRIDPEIVLAPAGVAALDAAI
                     FDAAHNHGHDPDIATFTVDLGPPLPRAAFERWLDTLLAHRGEDLLRVKGAVAFAGEAR
                     PMLVNIAGGLLHPLEPLDGDVSAPANLLVFITRRLPAAVIRGSLAAALDRLGAE"
     gene            complement(2679..3851)
                     /gene="metC"
                     /locus_tag="VGB88_12990"
     CDS             complement(2679..3851)
                     /gene="metC"
                     /locus_tag="VGB88_12990"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX9648412.1"
                     /translation="MKRDTVLVTAGRDPHANHGVVNPPVYHASTITFRTVAELEAKSR
                     DRFKAGVTYYGRYGTPTTFAFEEAVAAAEGGARAVAFGSGKAAIVAALTAFVEAGDHV
                     LMVDTAYGPTRALCERVLKRFGVETTYYDPLVGGRIGELIRPRTRVVFTESPGSQTFE
                     VQDIPAIAEAAHQAGCVVVMDNTWASPHLFKPFAHGVDVSIQAATKYIVGHSDAMMGV
                     VTTTEALHETVRAAAFDLGASAAPDDVYLALRGLRTLAVRLERHMRSALRIAEWLAGR
                     PEVHRVLHPARPGDAGHALWRRDFLGASGLFGVILEPAPKAALAAMLDRLELFAMGYS
                     WGGYESLIVPTDPAPLRTATRWQAPGPSLRLHVGLEDPDDLIADLEAGFARLNAAR"
     gene            complement(3944..5245)
                     /locus_tag="VGB88_12995"
     CDS             complement(3944..5245)
                     /locus_tag="VGB88_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX9648413.1"
                     /translation="MTARRKLSVTTEILIAMAAGAVLGILLNALFTDSAFVDDIVVDG
                     LFATVGGIFVAALKMLVVPLVFVSLVCGVTGLGDVAALGRIGVKALTLYLVTTAVAIT
                     IALIIAGIVAPGEGFEIDAGAVAYQGAEAPPLRKVITDLVPSNPVAAMAEGNMLQIII
                     FAIVLGVAVTLAGDGGRHVQNVFGDFNEVMMRMVGIVMRIAPIGVFCLIARTFASEGI
                     DVILPLASYFLTVVAALAAHAIGVYGLLLRVVGRLDPLVFYRKMRAAMVFAFSTASSN
                     ATIPVTLETVEHRLGVQNSVASFTVPLGATINMDGTAIMQGVATVFISHVYGVDLGLG
                     DYLTVILIAALASIGTAGVPGVGLIMLSMVLIQVGLPVEGIALIIGVDRLLDMMRTAV
                     NITGDSMVSCLVAGSEGALDRSRFADPDAGVIAGDVPAPAS"
     gene            complement(5367..>5579)
                     /locus_tag="VGB88_13000"
     CDS             complement(5367..>5579)
                     /locus_tag="VGB88_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9648414.1"
                     /translation="DGTLPAPAEIRRRFEAAGLDWARPVITTCGSGVTACILALGLHL
                     AGKDDWAVYDGSWAEWGARHDLPIER"
BASE COUNT          833 a         1994 c         2080 g          672 t
ORIGIN      
        1 gcgcagcttg cggggctcgg ccatggccgg ccggtcaggc gtcccgcgcc gagggcacgg
       61 ccgtcattgg cccgggtcgg gaaagaccac gcggtcgccg gcgacgatgc ccagccgctc
      121 ggtgatgccg cccttgagct cgagcacggc gcggaccggc ccgcccgacg agatcggctc
      181 cagcgacagc ggcgtggtcc aacgcgcgat gcgggtgatc gtgccgtcct tgccgacgaa
      241 caccatgtcg agcgggatca gcgtgttctt catccacatc gcgatcggct gcggctcgcc
      301 gaagtcgaac agcatgccgg cgtcgtcggc aagctcgccg cggaacatca gcccgcgcct
      361 ggtctgcgcg ggtgtccggg cgagctcgac atcgaagctg tggcgtccgc tgccggcctc
      421 gatcgacagg gtcgtgcgcg cgaaagtgat gtcggcgaca gccggcgcgg cgaggaccag
      481 cgccaaggcg gcgaccacga cgccgagcac gttgcgccgg cgcatcagtg cggcctcttt
      541 accagggtgg tgaagtcgaa cagcgccgcc tcgccgcccg cggtggcgca ggcgagatgg
      601 ctgccctggg gcgaccaggc gagcgcgctg accgcgtcct ccgcgccgtg gcggatcacc
      661 cggtcgcggc cgtggtcgag atcggcgagc agcacgctgc cgtcgtcgta gccggcggcg
      721 gcgagcggat ggcgcggatg gcaggcgagc gcggtcaccg cgcagccggc catcggcgcc
      781 accgccatcg gcacgtgcca gccgcgcagg tgcacgcgcc ggtcggtgcg ccagcacaac
      841 agcgcgccgt cgccggcgcc gaccagggcg cgcccgcagg cgctccaggc aagatcgcgg
      901 gccgagccga acagcgcgcc ggtgccggcg gcatcgccga ggtcccagac cagcgcggtg
      961 ggcgagtcgg tggcggcggc gagccggcgg ccgtagggcg agaaggcaag cgcatgcacc
     1021 ggcgcgctgg cgcttaacgc cgccgcagga gtttcccgcg ggcggtcgcg ccagaccgtg
     1081 acctcgcggc cgcgggccgc ggctaccgtg tctcccgacg ggctcgcagc gagcgcgctc
     1141 gcgccatcgc agccggcgcg ccaacccacg tggccggcgc ggtcgtagac gatcacctcg
     1201 ccggccgtcg ccaccgcccg cagccagccg ccggcgacca gccgggcgcc gccgtgggca
     1261 ccctgcgggt cgagcaacgc ggtggcggcg ccggcgccgt cgagctgcac cacccggccg
     1321 tcggcgccga ggctgagcac gccgccggtc gccccgtccg cgctcagcgc gcgcaccggg
     1381 ccatggtgga cggcggtcgc agtcatgcgc cggccgccga accgggccag cgccaccagc
     1441 cggacgctgc cgtcgtcgag cccgaacgcg gcatgggcgc cgttgccggc gaaggcggca
     1501 gccaaaaccg ggacatcgag cgtccagcgg gtgacggcga ccccgggcgc ggtggcgccc
     1561 tcaggccggc ccaggctgag cgccaccagc gccacggccg tcactccgcg ccaagacggt
     1621 cgagcgccgc cgccaaagag ccgcggatca ccgccgccgg cagcctgcgg gtgatgaaca
     1681 ccagcaggtt ggccggggcc gagacgtcgc cgtcgagcgg ttccagcggg tgcagcagcc
     1741 cgccggcgat attgaccagc atcggccgcg cctcgccggc gaaggcgacg gcgcccttga
     1801 cgcgcaacag gtcctcgccg cggtgggcga gcagggtatc gagccagcgc tcgaacgccg
     1861 cccgcggcag cggcggccca aggtcgacgg tgaaggtcgc gatgtccgga tcatggccgt
     1921 ggttgtgcgc ggcgtcgaag atggctgcat cgagcgctgc gacgccggcg ggcgcgagca
     1981 cgatctcggg atcgatccgc cccttgacca cccggtgccg cgcggccccc ggattgaccg
     2041 cggccagacg cgcctcgagg cgcgcgccgg cgtcggcgga ggcgaggtcg gtcttggtca
     2101 ccaggatgcg gtcggcgagc gcgacctggc gcagcgcctc gggcgcatcg tcgagccggc
     2161 ggaagccggt caccgcatcg accgtcacca ccaccgccgc cagccggtag cgcttggcga
     2221 gcgccgcggt ggcatgcagc gcgcgcacca ccggtaccgg gtcggcgagg ccggtggttt
     2281 ccaccaggac gcgcgcgaaa cccggcacgg tcccggcgtc gcgccggcgg gcgaggtcga
     2341 ccagcgcatg ctcgagaccg ccgcgcgcgg tgcagcagat gcagcccgat tcgagccgga
     2401 ccacctcgcc cgcgcgcccg cccaccagcg catcgtcgat cgccacctcg ccgaactcgt
     2461 tgatcaccac cgcgctgtcg gcgagcgcgg gccgcgcgag gagggccgcg agcagagtcg
     2521 tcttgccgct gccgaggaag ccggtcacca gggtgaccgg gcatctcgga tcgccgtcgg
     2581 gccggccggt catcacgcgc gcaccatcgc ctgtccgctc gccgataggg gaccagagcc
     2641 ggcgcgcgcg cgcaagccga accggcaggc gggcaggctc agcgggcggc gttgaggcgg
     2701 gcgaagccgg cctcgaggtc ggcgatcagg tcgtccgggt cctcgagccc gacatgcagg
     2761 cgcaggctcg gccccggtgc ctgccagcgg gtcgcggtgc gcagcggcgc cgggtcggtg
     2821 ggcacgatca ggctctcgta gccaccccag gaatagccca tcgcgaacag ctcgagccgg
     2881 tcgagcatcg ccgccagcgc ggccttcggg gcgggctcca ggatcacgcc gaacaagccg
     2941 ctcgcgccca ggaagtcgcg ccgccacagc gcatgtccgg cgtcgcccgg acgcgccgga
     3001 tgaagcacgc gatggacctc gggccgcccg gcgagccact cggcgatgcg gagcgcgctc
     3061 cgcatgtggc gctcgaggcg cacggcgagc gtgcgcaggc cgcgcagcgc cagatagacg
     3121 tcgtcgggcg ccgccgaggc gccgaggtcg aaggccgccg cgcgcaccgt ctcgtgcagc
     3181 gcctcggtag tggtgacgac gcccatcatg gcgtcggagt ggccgacaat gtacttggtc
     3241 gcggcctgga tcgagacatc gacgccgtgc gcgaacggct tgaacaaatg cgggctcgcc
     3301 caggtgttgt ccatcaccac cacgcagccc gcctggtgcg cggcctcggc gatcgccggg
     3361 atgtcctgga cctcgaaggt ctgcgagccg ggggactcgg tgaacacgac cctggtccgg
     3421 ggtcggatca gctcgccgat ccgcccgccg accagcgggt cgtaataggt ggtctcgacg
     3481 ccgaagcgct tgagcacgcg ctcgcacagc gcgcgcgtcg gcccataggc ggtatcgacc
     3541 atcagcacgt ggtcgccggc ctcgacgaag gccgtgagcg cggcaacgat cgccgccttg
     3601 cccgagccga acgcaaccgc gcgcgcaccg ccctcggctg ccgccaccgc ctcctcgaac
     3661 gcgaacgtgg tcggcgtgcc gtagcggccg taataggtga cgccggcctt gaagcggtcg
     3721 cggctcttgg cttcgagctc ggcgacggtg cggaaggtga tggtcgaggc gtggtagacg
     3781 ggcgggttga ccacgccgtg gttggcgtgc gggtcgcgcc ccgcggtgac gagtacggta
     3841 tcgcgcttca tggcttcgct gtccgtcgtg tcgggccgcg ggcactatgt cacccgcggc
     3901 gtggcggcgg aagcgccgtc gggctaaggc gcggtcggtg cgctcaggac gcgggcgcgg
     3961 gcacgtcgcc ggcgatgacg ccggcgtcgg ggtcggcgaa gcgcgacctg tcgagcgccc
     4021 cctcgctgcc cgcgaccagg cagctgacca tcgaatcgcc ggtgatgttg accgcggtgc
     4081 gcatcatgtc gagcagcctg tcgacgccga tgatcagcgc gatgccctcg accggcagcc
     4141 cgacctggat cagcaccatc gacagcatga tcagccccac ccccggtacg ccggcggtgc
     4201 cgatcgaggc gagcgccgcg atcaggatga cggtcaggta atcgccgagc ccgaggtcga
     4261 cgccgtagac gtggctgatg aacacggtcg ccacgccctg catgatcgcg gtgccgtcca
     4321 tgttgatggt ggcgccgagc ggcaccgtga acgaggccac cgagttctgc accccgaggc
     4381 gatgctcgac cgtctccagg gtcaccggga tggtggcgtt ggagctcgcg gtgctgaacg
     4441 cgaacaccat tgccgcccgc atcttgcgat agaagaccag cgggtcgagg cggccgacga
     4501 cccgaagcag caggccgtaa acgccgatcg catgggccgc cagcgccgcg accacggtca
     4561 ggaagtagct cgccagcggc aggatgacgt cgatgccctc gctggcgaag gtgcgggcga
     4621 tcaggcagaa cacgccgatc ggcgcgatgc gcatgacgat gcccaccatc cgcatcatca
     4681 cctcgttgaa gtcgccgaag acgttctgca cgtggcggcc gccgtcgccg gccaaggtca
     4741 cggcgacgcc gaggacgatg gcgaagatga tgatctgcag catgttgcct tcggccatcg
     4801 ccgccaccgg gttgctgggg acgaggtcgg tgatcacctt gcgcaagggc ggcgcctcgg
     4861 cgccctggta cgcgaccgcg ccggcgtcga tctcgaagcc ctcgccgggc gcgacgatgc
     4921 cggcgatgat cagggcgatg gtgatggcga cggcggtggt gaccagataa agcgtcagcg
     4981 ccttgacgcc gatgcgcccg agcgcggcaa cgtcgccgag cccggtcacg ccgcagacca
     5041 gcgacacgaa caccagcggc acgaccagca tctttagcgc cgcgacaaag atgccgccga
     5101 cggtggcgaa cagcccgtcg accacgatgt cgtcgacgaa tgcggagtcc gtgaacagcg
     5161 cgttgagcag gatgccgagc accgccccgg ccgccatcgc gatcaggatc tcggtggtca
     5221 cgctgagctt gcgtcgggcg gtcatgcagc ctccgtgacg gacccgcggc cgatcgcgtt
     5281 ggtagcacgg cgggcgccgc atcgcgaccc cggcgccgac ttgggaggcg cgctacggtg
     5341 gcgtcaatgc gtctcggcgg ggccgatcag cgctcaatgg gaagatcatg gcgggcgccc
     5401 cattcggccc acgagccgtc atagaccgcc cagtcgtcct tgccggcgag gtgcaggccg
     5461 agcgcgagga tgcaggcggt gacgcccgag ccgcaggtgg tgatcaccgg gcgggcccag
     5521 tcgaggccgg cggcctcgaa ccggcggcga atctcggccg gggcgggcag ggtgccgtc
//
LOCUS       DASWHU010000350         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_215211, whole genome shotgun sequence.
ACCESSION   DASWHU010000350 DASWHU010000000
VERSION     DASWHU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215211"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VGB88_13005"
     CDS             complement(<1..865)
                     /locus_tag="VGB88_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9648415.1"
                     /translation="MDVSSELVHSLLPVFMVTVLGASAVAVGAVEGAAEATASVTKVL
                     SGWLSDRLGRRKALALAGYGLAAVAKPLFALAPTIGWVFAARVIDRLGKGVRGAPRDA
                     LVADLSPPAVRGASYGLRQSLDSVGAFAGPLAAVGLMAAFADDMRIVFWFALPPALIA
                     VAVLAWFVREPERAAPASPAPAPGSSPGVRLADLGRLGGAYWRVVAVGAALALARFSE
                     AFLVLRAADVGLALAWVPMVLVVMSAAYALSAFPAGLIADRAGRKLPFALGCAALVAA
                     DLVLAAAGGVAG"
     gene            complement(943..>3576)
                     /gene="alaS"
                     /locus_tag="VGB88_13010"
     CDS             complement(943..>3576)
                     /gene="alaS"
                     /locus_tag="VGB88_13010"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9648416.1"
                     /translation="RSHFLDYFRRHGHEVVASSPLVPRNDPTLLFTNAGMVQFKNVFT
                     GQETRPYKRAATAQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKELAIEL
                     AWTLVTRELGLDKDRLLVTVFHTDDEAAGHWKKIAGLPDERIIRIPTADNFWAMGDTG
                     PCGPCSEIFYDHGPGIPGGPPGSPDADGDRFIEIWNLVFMQFEQVTKQERVPLPRPSI
                     DTGMGLERVTAVLQGKHDNYDIDLMRALIEASAEASGQAADGAHAVSHRVIADHLRAA
                     AFLIADGVLPSNEGRGYVLRRIMRRAMRHAHRLGVKEPLMHRLVPALTAEMGQPYPEL
                     LRAEPLIRETLRLEESRFKETLERGLRLLDEALAALGPGQALPGETAFKLYDTYGFPL
                     DLTQDILRQQQRSVDTAGFDAAMERQRAAARKTWAGSGEAATETLWFDIRERVGATEF
                     FGYDTLSAEAIVKAVVVGKQEVAAAEAGQEVAVVVNQTPFYGEAGGQMGDAGVLFTAG
                     GTEVAIRDTQKRLGDLVVHLGQVTRGALKLGDTVELRVDGERRDRLRANHSATHLLHA
                     ALRRRLGPHVTQKGSLVAPDRLRFDISHPKPVGDDELGAIEREVNAEILANTPVQTLL
                     MTPSEAVEAGALALFGEKYGAEVRVLRIGGAPQAPYSVELCGGTHVARTGDIGLFKVV
                     GEGAVAAGVRRIEGVTGEGALAYVNAQAAALREAAAVLRTQPAELPARIAQLLEERKR
                     LERELTEARRALATGGAGAGAETRRIAGVAFAARKLDGVPAKELRGMADAMKKQLGSG
                     VVMVVSATDGKGAAVVGVTDDLTGRIDAVDLVRAAAQALGGKGGGGRPDMAQAGGPDG
                     AGADAAIAAVEAALAARAEAA"
BASE COUNT          488 a         1330 c         1305 g          453 t
ORIGIN      
        1 cgcccgccac cccgcccgcg gccgcgagca ccaggtcggc cgccaccagc gcggcgcagc
       61 cgagcgcgaa cggcagcttg cggccggcgc ggtcggcaat gagcccggcg gggaacgccg
      121 acagcgcgta ggccgcgctc atcaccacca gcaccatcgg cacccaggcg agcgccaggc
      181 cgacatcggc cgcgcgcagc accaggaacg cctcgctgaa acgcgccagc gcaagcgcgg
      241 cgccgaccgc cacgacacgc caataggcgc cgcccagccg accgagatcg gcgagacgga
      301 cccccgggct agacccgggg gccggcgccg ggcttgccgg ggcggcgcgt tcgggctcgc
      361 gcacgaacca cgccagcacc gcgaccgcga tcagtgccgg cggcagcgcg aaccagaaca
      421 cgatgcgcat gtcgtcggcg aaggcggcca tcagcccgac cgcggccagc ggcccggcga
      481 acgcgcccac cgagtcgagc gactggcgca ggccgtagct cgcgccgcgt acggcgggcg
      541 gcgacaggtc cgcgaccagc gcatcgcgcg gcgcgccgcg cacgcccttg ccgagccggt
      601 cgatgacgcg ggcggcgaac acccagccga tggtcggcgc gagcgcgaac agcggcttgg
      661 ccacggcggc gaggccgtag ccggcgagcg ccagcgcctt gcgccggccg agccggtcgc
      721 tcagccaccc cgacaggacc ttggtcaccg acgcggtcgc ctcggcggcc ccttcgacgg
      781 caccgaccgc gaccgcgctc gcgccgagca cggtaaccat gaacaccggc agcaggctgt
      841 gcaccagctc ggacgagacg tccatgaaca ggctgacgaa accgagcgcc caaaccccgc
      901 gcgggaccgc gcgccacgcc gtgccggccg tgcgggactc cgtcaggcgg cttcggcgcg
      961 ggcggcgagc gcggcctcga ccgcggcgat ggccgcgtcc gcgcccgcgc cgtcggggcc
     1021 gccggcctgg gccatgtcgg gccgcccgcc gccgcccttg ccgccgagcg cctgggcggc
     1081 ggcgcgcacc aggtcgacgg cgtcgatgcg cccggtcagg tcgtcggtga cgccgaccac
     1141 cgccgcgccc ttgccgtcgg tggcggagac caccatgacc acgcccgagc cgagctgctt
     1201 cttcatcgcg tccgccatgc cgcgcagctc cttggccggc accccgtcga gcttgcgcgc
     1261 ggcgaaggcg acgccggcga tgcggcgggt ctcggcgccc gcgccggcgc cgccggtggc
     1321 gagcgcgcgc cgcgcctcgg tgagctcgcg ctcgagccgc ttgcgctcct ccagcagctg
     1381 ggcgatgcgc gccggcagct ccgcgggctg ggtcctgagc acggccgccg cctcgcgcag
     1441 cgccgcggcc tgcgcattga cgtaggcgag cgcgccctcg ccggtcaccc cctcgatccg
     1501 gcgcacgccg gcggcgaccg cgccctcgcc cactaccttg aacaggccga tgtcgccggt
     1561 gcgcgcgacg tgggtgccgc cgcacagctc gaccgagtac ggcgcttggg gcgcgccgcc
     1621 gatcctgagc acgcgcacct ccgcgccgta cttctcgccg aacaaagcga gcgcgccggc
     1681 ctcgaccgcc tcggacgggg tcatcagcag cgtctggacc ggcgtgttgg ccaggatctc
     1741 ggcgttcacc tcgcgctcga tcgcacccag ctcgtcgtcg cccaccggct tcgggtggct
     1801 gatgtcgaag cgcagccggt cgggggcgac cagcgagccc ttctgcgtca cgtgcgggcc
     1861 gagccgccgc cgcagcgcgg cgtgcagcag gtgcgtcgcc gaatggttgg cgcgcaggcg
     1921 gtcgcgccgc tcgccgtcga cgcgcagctc gacagtatcg cccagcttca gcgcgccgcg
     1981 ggtgacctga cccaggtgca cgacgagatc gcccaggcgc ttctgcgtat cgcggatcgc
     2041 cacctccgtg ccgccggcgg tgaagaggac gccggcatcg cccatctggc cgccggcctc
     2101 gccgtagaag ggggtctggt tgacgacgac cgccacctcc tggccggctt ccgccgcggc
     2161 gacctcctgc ttgccgacga cgaccgcctt gacgatggct tcggcggaaa gcgtgtcgta
     2221 gccgaagaac tcggtggccc cgacccgctc gcggatgtcg aaccagagcg tctcggtcgc
     2281 cgcctcgccg gagccggccc aggtcttcct ggcggcggcg cgctggcgct ccatcgcggc
     2341 atcgaagccg gcggtgtcca cgctgcgctg ctgctggcgc aggatgtcct gcgtcaggtc
     2401 gagcgggaag ccataggtgt cgtagagctt gaaggcggtc tcgcccggca gcgcctggcc
     2461 cgggccgagt gcggccagcg cctcgtccag cagcctgagg ccgcgctcca aggtctcctt
     2521 gaagcggctc tcctccaggc gcagcgtttc gcggatcaag ggttcggcgc gcagcagctc
     2581 cggatagggc tggcccatct ccgcggtcag cgccggcacc agccggtgca tcagcggctc
     2641 cttcaccccc aggcggtgcg cgtggcgcat ggcgcggcgc atgatccggc gcagcacgta
     2701 gccgcggccc tcgttggacg gcagcacgcc gtcggcgatc aggaaggcgg cggcgcgcag
     2761 gtggtcggcg atgacccggt gcgagacggc gtgggcgccg tcggccgcct ggccggaggc
     2821 ctcggcggag gcctcgatca gcgcgcgcat caggtcgatg tcgtagttgt cgtgcttgcc
     2881 ctgcagcacg gcggtgacgc gctcgagccc catgccggtg tcgatcgagg ggcgcggcag
     2941 cggcacgcgc tcctgcttcg tcacctgctc gaactgcatg aacaccaggt tccagatctc
     3001 gatgaagcgg tcgccgtcgg catcggggct gccgggcggg ccgccgggga ttccggggcc
     3061 gtggtcgtag aagatttccg aacaggggcc gcaggggccg gtatcgccca tcgcccagaa
     3121 gttgtcggcg gtcggaatgc ggatgatgcg ctcgtcgggg agcccggcga tcttcttcca
     3181 gtggccggcc gcctcgtcgt cggtgtggaa gacggtaacc agcaggcggt ccttgtcgag
     3241 gccgagctcg cgggtgacca gcgtccaggc cagctcgatc gccagctcct tgaagtagtc
     3301 gccgaaggag aagttgccca gcatctcgaa gaaggtgtga tggcgggcgg tgtagccgac
     3361 attctccagg tcgttgtgct tgccgccggc gcgcacgcac ttctgcgcgg tggcggcgcg
     3421 cttgtagggc cgcgtctcct ggccggtgaa gacgttcttg aactgcacca tgccggcgtt
     3481 ggtaaacagc agcgtcggat cgttgcgcgg caccagcggc gaggaggcca ccacctcgtg
     3541 cccgtggcga cggaagtagt cgaggaaatg actgcg
//
LOCUS       DASWHU010000351         7079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2090331, whole genome shotgun sequence.
ACCESSION   DASWHU010000351 DASWHU010000000
VERSION     DASWHU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090331"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..325
                     /locus_tag="VGB88_13015"
     CDS             56..325
                     /locus_tag="VGB88_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9648417.1"
                     /translation="MGRISFTANLQRHVSCPTAEVRAATVREALDEVLAGNPRLRPYL
                     LDEHGRLRRHVNVFVNDRAVADRIGLSDRVAPEDEIYVFQALSGG"
     gene            329..1411
                     /locus_tag="VGB88_13020"
     CDS             329..1411
                     /locus_tag="VGB88_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX9648418.1"
                     /translation="MSDIFHVASRKGLFRFRRQGSRWVGGAPAFVGEPVSAVFADRRD
                     GALYCALRLGHFGVKLHRSDDGGDSWTEVAAPAFPARPASDESAPAVDMIWTLVAGGA
                     DEPGMLWAGTLPAALFRSADRGESWSLIDGLWTVPERAEWQGGGYDHAGIHSIHVDAR
                     DSGRITLAISIGGVWKSGDAGAAWRLVGAGLRAEYMPPAKAYDPVVQDPHRLAHCAAA
                     PDVVWCQHHNGIFRSTDGGATFDEIAPVRPSAFGFAVAAHPQNPECAWFVPAVKDECR
                     VPVAGRLVVTRTTDGGRSFTALGEGLPSRDCYDLVYRHGLDVDATGARLAMGSTTGNL
                     WTSDDGGDHWTQVSAHLPPIAQVAFG"
     gene            1533..2636
                     /locus_tag="VGB88_13025"
     CDS             1533..2636
                     /locus_tag="VGB88_13025"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9648419.1"
                     /translation="MDILRPTVVALAGALALSLAANGAAGQSAAERDAVARSKAMVPT
                     LPWPQGDQRGMANAIGPGTWLRCAQHLTEPNAKVYEISHVRSNTMPLSPFGVPLAYEY
                     RPTVGIPGTLHAFNGEQVKSGEPGAQGTQMDAIGHFAYLAQPWDGQGDFPAGDAKYYG
                     GYTQAEVKPSPDSPLQKLGIENVPPLITSAVLLDAKSYLGGGQPMQPGQQVTAADIEA
                     MVEAQGLGWRGILPGDVLYIYTGWGDNWKDPDTEKSYYTMGPGLAFDAAEYIEKKGVV
                     LVALDNPFTDPVNQGQLMGQAGPPEGTPPGLPFVIHHYNLAMAGIHQIQNANLGALAK
                     DKVWTSCTIILPLRSSGGSGSPVRPVAIGAPAG"
     gene            2761..3120
                     /locus_tag="VGB88_13030"
     CDS             2761..3120
                     /locus_tag="VGB88_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9648420.1"
                     /translation="MGASSATLHESQDRLDAATVDRHRAIVSLMEELEAVDWYDQRVA
                     ATGDDSLRAVLAHNRDEEKEHAAMILEWLRRHDDALDRMLRSYLFTDQPVTAIEQGGE
                     APPAPADGSLGIGDLRG"
     gene            3129..3977
                     /locus_tag="VGB88_13035"
     CDS             3129..3977
                     /locus_tag="VGB88_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX9648421.1"
                     /translation="MDDLHRELAPVSDAAWTEIEDEARRTVKLALAGRKVVDFDGPRG
                     WDCASVSLGRTEALAQAPAGGIEARLRRVQRLVELRAPFTLRRDELESAARGGRDPDL
                     KPVTDAARTIAAAEDHAIFHGYPAGGIKGIAEGAAHATLALSDDYERYPKVVAEALSH
                     LRHAGVAGPYAIALGPRCYKGLTETTTAGGMRVFDHVRNLLDGPVVWAPAVNGAVIAS
                     MRGGDFVLTVGQDLSIGYLGHDADTVSLYLQESMTFRVLSGEAAVPLSYQAQPRAGGR
                     RAARGR"
     gene            3993..4799
                     /locus_tag="VGB88_13040"
     CDS             3993..4799
                     /locus_tag="VGB88_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648422.1"
                     /translation="MTGASGDLGRVLTDGLRDKGYRLRLSDIVARDDVPAECAFVRAD
                     LADKDAVLGLCEGVDGIAHFGAISRENTFEAILEANIRGTYHIFEGARLNRARVVFAS
                     TNHTIGFHERSERLAEDCHFRPDSYYGLSKAYGELLGRHYWDKHGVESAHLRIGSCFA
                     RPADVRQLSTWLSFADLVELVDGCFRCEDLGCATFWGVSANERRWWTDHARELIRFTA
                     ADNAETFKDRVPPVPADADPIAERYQGGVFCADGYSRATPAPKVLFRTSR"
     gene            4885..6090
                     /gene="pcaF"
                     /locus_tag="VGB88_13045"
     CDS             4885..6090
                     /gene="pcaF"
                     /locus_tag="VGB88_13045"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX9648423.1"
                     /translation="MSEAFICDSARTAIGRYGGALSAIRTDDLAAVPITALIARNVGV
                     DWDAVDDVVYGDANQAGEDNRNVARMAALLAGLPDSVPGQTVNRLCGSGLQAVASAAH
                     AIRAGDARLMIAGGVEGMTRAPFVMAKSEQAFARNLEVYDSTIGWRFVNARMKAAHGI
                     DSMPETAENVAEEYQVSRADQDKFALRSQQRAGAAIAAGRLAEEIVPVAIPQRKGAPV
                     IVDRDEHPRPDTTLDKLAGLATPFRDGGTVTAGNASGVNDGAAAIVIASEQAAARWGL
                     QPIARVLGAASAGVAPRIMGMGPAPATRKLLDRLGLAVSDMDVIELNEAFAAQALAVL
                     RELGLADDAEQVNPNGGAIALGHPLGMSGARLVLTAARELARRKRRYALCTMCIGVGQ
                     GIAMVVERV"
     gene            6120..>7079
                     /locus_tag="VGB88_13050"
     CDS             6120..>7079
                     /locus_tag="VGB88_13050"
                     /inference="COORDINATES: protein motif:HMM:NF023426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX9648424.1"
                     /translation="MNILFIHQNFPAQYRHIALSYAREGGHGLVAVGKDTAGRVKGVR
                     HVLYKLTRPPRQSRHPYVHDFETKVHHGQTVRRALAAIKERGFLPDVILAHPGWGEDM
                     FVPDVFPEVPRLLYAEFYYHAHGADVNFDPEQPLADGDDARIRARNAGQLLSLESATW
                     GVSPTEWQRSQYPELFRPRISVLHDGIDVDLVRPDDGASLVLAGGTTLTRADEVVTYA
                     VRNLEPYRGFHQFLRAARLILERRPRAQIVVVGGDEVSYGRRPPAGTTFREHYLAEAP
                     IDQARVHFLGRLPYDQYLRVLQVSQAHIYLTVPFVLSWSMLEAM"
BASE COUNT         1044 a         2528 c         2516 g          991 t
ORIGIN      
        1 cgcatcgacc catcgcgccg gcgtaagacc agaggtacag agttgagggc ccgcgatggg
       61 gcggatcagc ttcacagcca acttgcagcg ccacgtgtcg tgcccgaccg ccgaagtgcg
      121 cgccgccacg gtgcgcgagg cgttggacga ggtccttgcc ggcaatccgc ggctgcgccc
      181 ctatctgctc gacgaacacg gccgcctgcg ccggcacgtg aacgtgttcg tcaacgaccg
      241 cgcggtcgcg gaccgcatcg ggttgagcga ccgcgtcgcg cccgaggacg agatctacgt
      301 cttccaagcc ctttcgggag ggtgagccgt gtcggacata ttccacgttg ccagccgcaa
      361 gggtttgttt cgctttcgcc ggcaaggctc gcgctgggtg ggcggggcgc ccgcgttcgt
      421 cggcgagccg gtctcggcgg tctttgccga tcgccgcgac ggcgcgctct attgcgcgct
      481 taggctcggc catttcggag tcaagctgca ccgctccgac gacggcggcg acagctggac
      541 ggaagtcgcc gcgcccgcct ttccggcgcg gccggcgtcg gacgagtcgg caccggcggt
      601 ggacatgatc tggacgctgg ttgccggcgg tgcggacgag ccggggatgt tgtgggccgg
      661 cacgttgccg gcggcgctgt tccgcagcgc cgaccgcggc gaaagctgga gcttgatcga
      721 cggcctgtgg accgtgcccg agcgcgccga gtggcaaggc ggcggctacg accatgccgg
      781 aatccattcg atccacgtcg atgcgcgcga cagcgggcgg atcacgctcg ccatctccat
      841 cggcggcgtc tggaagagcg gcgacgcggg cgccgcttgg cgtctcgtcg gcgcgggcct
      901 gcgcgccgaa tacatgcccc cggccaaggc gtacgacccg gtcgtccagg acccgcaccg
      961 cctcgcccat tgcgcggcgg cccccgacgt tgtctggtgc caacaccaca acggcatttt
     1021 tcgctcgacc gacggcgggg cgacattcga cgagatcgct cccgtccggc catcggcgtt
     1081 cggcttcgcc gtggccgcgc acccgcagaa tcccgagtgc gcgtggttcg tgccggcggt
     1141 caaggacgag tgccgcgtgc cggtggctgg gcgcctcgtg gtcacccgca ccaccgacgg
     1201 cgggcgtagc ttcacggcgt tgggcgaggg gctgccgtcg cgcgactgct acgacctcgt
     1261 ctaccggcac ggcctcgacg tcgacgcgac cggcgcgcgg ctggcgatgg gctcgaccac
     1321 gggtaatctg tggaccagcg acgacggcgg cgaccactgg acccaggtca gcgcccatct
     1381 gcccccgatt gcgcaggtgg cgttcggctg acgccgcggc cggcgcccac ttgccaaccg
     1441 gtccgcgtcc gcccaaactc gcgccgcacg acaccaagcc ggccgcagtg ccggcgggct
     1501 ttgcactgtg caggaccgga gggaggacga cgatggacat cttgagaccc acagtggtgg
     1561 cgctcgcggg ggcattggcg ctgagcctgg ccgcgaacgg cgccgccggc cagtcggctg
     1621 ccgagcgcga cgcggtcgca cgctcgaagg cgatggtgcc gacgctgccg tggccgcaag
     1681 gcgaccagcg cggcatggcc aacgcgatcg ggccgggcac ctggttgcgc tgcgcgcagc
     1741 acctgaccga gccgaacgcc aaggtctacg agatctcgca tgtgcgctcg aacaccatgc
     1801 cattgtcgcc gttcggcgtg ccgttggcgt atgagtaccg gccgaccgtc ggcatcccgg
     1861 ggaccctgca tgcgttcaac ggcgagcagg tgaagagcgg cgagccgggc gcccagggca
     1921 cgcagatgga cgcgatcggg cacttcgcct atctcgccca gccgtgggac gggcagggcg
     1981 acttcccggc cggcgacgcg aaatattacg gcggctatac ccaggccgag gtgaagccgt
     2041 cgcccgactc gccgctgcag aagctcggta tcgagaacgt gccgccgctc atcaccagcg
     2101 cggtgctgct cgatgccaag agctatctcg gcggcggcca gccgatgcag ccgggccagc
     2161 aggtcaccgc ggccgacatc gaggcgatgg tcgaggcgca gggcctcggc tggcgcggca
     2221 tcctgccggg cgacgtgctc tacatctaca ccggctgggg cgacaactgg aaggacccgg
     2281 acaccgagaa gtcctactac accatgggcc cggggctcgc gttcgatgcc gccgagtaca
     2341 tcgaaaagaa gggcgtggtg ctggtcgccc tcgacaaccc gttcaccgat ccggtcaacc
     2401 agggccagct gatgggccag gccggcccgc ccgagggcac gcccccgggc ctgccgttcg
     2461 tgatccacca ttacaacctg gccatggcgg gcatccatca gatccagaac gccaatctgg
     2521 gcgcgctcgc caaggacaag gtgtggacct cgtgcaccat catcctgccg ctgaggtcga
     2581 gcggcggctc gggctcgccg gtccgtccgg tggcgatcgg cgcgccggcc ggatagcggc
     2641 ctatgcgaag cggtggcggc gcgctgccgc cgccgctacg ccccgtttcg tcttgtccgg
     2701 ctcggcggat gcccctactg tcgcgacggc gcgacatcac ggaaaagctc ggggacagcc
     2761 atgggcgcga gcagcgcgac actgcacgaa tcgcaagacc gcctggacgc ggcgacggtg
     2821 gacaggcatc gcgccatcgt ctcgctgatg gaggagctcg aggcggtcga ctggtacgac
     2881 cagcgggtgg ccgcgaccgg cgacgactcc ttgcgtgcgg tcctcgcgca caaccgcgac
     2941 gaggagaagg agcacgccgc gatgatactc gagtggctgc gccgccatga cgacgccctc
     3001 gaccggatgc tgcgcagcta tctgttcacc gaccagccgg tcaccgctat cgagcaaggc
     3061 ggcgaggcgc cgccggcgcc cgccgacggc tcgctcggga tcggcgacct gcgcggataa
     3121 gcgctgccat ggacgacctg cacagagagc tcgccccggt ctccgacgcc gcctggacgg
     3181 agatcgagga cgaggcgaga cggacggtca agctcgcgct cgcggggcgc aaggtcgtcg
     3241 acttcgacgg tccgcgcggc tgggactgcg cgtcggtctc gctcgggcgc accgaagcgc
     3301 tggcgcaggc gccggccggc ggcatcgagg cgcgcctgcg ccgcgtgcag cggctggtcg
     3361 agctgcgcgc cccgttcacc ctgcgccgcg acgagctcga gagcgccgcg cgcggcggac
     3421 gcgaccccga cctcaagccg gtgaccgacg cggcgcggac catcgccgcc gccgaggacc
     3481 acgccatctt ccacggctac cccgcgggcg gcatcaaggg catcgccgag ggcgccgcac
     3541 acgccacgtt ggcgctcagc gacgactacg agcgctaccc caaggtggtc gccgaggcct
     3601 tgagccatct gcgccacgcc ggcgtcgccg gtccctatgc cattgcgctc gggccgcgct
     3661 gctacaaggg cctgaccgag accaccaccg ccggcggcat gcgcgtgttc gaccacgtcc
     3721 gcaatcttct cgacggcccg gtggtgtggg cgcctgcggt caacggcgcg gtgatcgcca
     3781 gcatgcgcgg cggcgacttc gtgctcaccg tcggccagga cctgtcgatc ggctatctcg
     3841 gccacgacgc cgacacggtg agcctgtacc ttcaggagag catgacgttc cgcgtgctgt
     3901 cgggcgaggc ggcggtgccg ctgtcgtacc aggcgcagcc ccgcgccggc gggcgccgcg
     3961 cggcgcgcgg gcggtaaatg ccgaccatcc tgttgaccgg ggcctccggc gacctcggcc
     4021 gcgtgctgac cgacggcttg cgcgacaagg gctatcggct ccgcctgtcc gacatcgtgg
     4081 cgcgcgacga cgtgccggcg gagtgcgcgt tcgtgcgcgc cgaccttgcc gacaaggacg
     4141 cggtgctcgg cctgtgcgag ggcgtcgacg gcatcgcgca tttcggcgcc atctcgcgcg
     4201 agaacacgtt cgaggcgatc ctcgaggcca acatccgcgg cacctatcac atcttcgagg
     4261 gcgcccggct caatcgcgcg cgggtcgtgt tcgccagcac caaccacacc atcggtttcc
     4321 acgagcgcag cgagcgattg gccgaggact gccatttccg ccccgacagc tattacggcc
     4381 tgagcaaggc gtacggcgag ctgctcggcc gccactactg ggacaagcat ggggtcgaga
     4441 gcgcgcacct gcgcatcggc tcgtgcttcg cgcggccggc cgacgtgcgc cagctcagca
     4501 cctggctgag ctttgccgac ctggtcgagc tggtcgatgg ctgcttcagg tgcgaggacc
     4561 tcggctgcgc aaccttctgg ggcgtctcgg cgaacgagcg caggtggtgg accgaccacg
     4621 cgcgcgagct gatccgcttc accgccgccg acaacgccga gacgttcaag gaccgggtgc
     4681 cgccggtgcc ggccgacgcc gacccgatcg ccgagcgcta tcagggcggc gtcttctgcg
     4741 ccgacggcta cagccgcgcg acgcccgcgc ccaaggtcct gttccgcacc tcgcgctgat
     4801 cgccggcggc cggattgccg gcgcccgggt caatgtgcat agtgccgtga tccagcgcgg
     4861 ccctggcggg acgggagcgg agcgatgtcc gaagccttca tctgcgactc cgcgcgcacg
     4921 gcgatcgggc gctatggcgg cgcgctgtcc gcgatccgca ccgacgacct cgccgcggtg
     4981 ccgatcacgg cgctgatcgc gcgcaatgtc ggcgtcgatt gggacgcggt cgacgacgtc
     5041 gtctacggcg acgccaacca ggcgggcgag gacaaccgca acgtcgcgcg catggcggcg
     5101 ctgctcgcgg gcctccccga ctcggtgccc ggccagaccg tcaaccggct gtgtggctca
     5161 gggctccagg cggtggcgag cgcggcgcac gcgattcgcg cgggcgacgc ccggctgatg
     5221 atcgcgggcg gggtcgaggg catgacccgc gcgccgttcg tgatggccaa gagcgagcag
     5281 gcgttcgcgc gtaacctgga ggtctacgac agcaccatcg gctggcgctt cgtcaatgcg
     5341 cgcatgaagg cggcgcacgg catcgactcg atgcccgaaa ccgccgagaa cgtcgccgag
     5401 gagtaccagg tctcgcgcgc cgaccaggat aagttcgcgc tccgctcgca gcagcgcgcg
     5461 ggcgccgcga tcgccgccgg ccgcttggcc gaggagatcg tgccggtggc gattccccaa
     5521 cgcaagggcg cacccgtgat cgtcgaccgc gacgagcacc cgcgccccga caccaccctc
     5581 gacaagctgg ccggcctggc gacgccgttc cgcgacggcg gcacggtcac cgcgggcaac
     5641 gcctcgggag tcaacgacgg cgccgcggcg atcgtcatcg cctcggagca ggcggcggcc
     5701 cgctggggct tgcagccgat agcgcgcgtc ctcggcgcgg cgagcgccgg cgtcgcgccc
     5761 aggatcatgg gcatggggcc ggcgccggcg acgcgcaagc tgctcgaccg gctcgggctc
     5821 gcggtctccg acatggacgt gatcgagctc aacgaggcgt tcgccgccca ggcgctggcg
     5881 gtgctgcgcg agctcggcct cgccgacgac gccgagcaag tcaaccccaa tggcggcgcg
     5941 atcgcgctcg gccacccgct cggcatgagc ggcgcccggc tggtgctgac cgcggcgcgc
     6001 gagcttgccc gccgcaagcg gcgctacgcg ctgtgcacca tgtgcatcgg cgtcggccag
     6061 ggcatcgcaa tggtggtcga gcgggtctag gcgcttcgcc aagccgcgac cgggtcggca
     6121 tgaacatcct gttcatccac cagaactttc ccgcccaata ccgccacatc gcgctgtctt
     6181 atgcgcgcga gggcggccac gggctggtgg cggtcggcaa ggacacggcg ggccgggtga
     6241 agggggtgcg ccacgtgctc tacaagctca cgcggccgcc gcgccagagc cgccaccctt
     6301 atgtgcacga cttcgagacc aaggtgcacc acggccagac cgtgcgccgg gcgctggcgg
     6361 cgatcaagga gcgcggcttt ctccccgacg tgatcctcgc tcatccgggc tggggcgagg
     6421 acatgttcgt gcccgacgtc ttccccgagg tgccgcgcct gctttatgcc gagttctact
     6481 atcacgccca cggcgccgac gtgaacttcg accccgagca accgctggcg gacggcgacg
     6541 acgcgcgcat ccgcgcacgc aatgccggcc agctcttgag cctggagagc gcgacctggg
     6601 gggtgagccc gaccgagtgg cagcgaagcc agtacccgga gctgttccgc ccgcgcatct
     6661 cggtgctgca cgacggcatc gacgtcgacc tggtgcgccc cgacgacggc gcgagcctcg
     6721 tcctcgccgg cggcaccacg ctgacccgcg ccgacgaggt ggtcacctat gcggtgcgca
     6781 accttgagcc ctatcgcggc ttccaccagt tcctgcgcgc ggcgcggctg atcctcgagc
     6841 gccggccgcg ggcgcagatc gtggtcgtcg gcggcgacga ggtgagctac ggccgccgcc
     6901 cgcccgcggg cacgaccttc cgcgagcact acctggccga ggcgccgatc gatcaggcgc
     6961 gcgtccactt cctgggccgg ctgccttacg accagtacct gcgggtgctc caggtgtcgc
     7021 aggcacacat ctatctgacg gtgccgttcg tgctgtcgtg gtcgatgctg gaggcgatg
//
LOCUS       DASWHU010000352         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_221136, whole genome shotgun sequence.
ACCESSION   DASWHU010000352 DASWHU010000000
VERSION     DASWHU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221136"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VGB88_13055"
     CDS             <1..317
                     /locus_tag="VGB88_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX9648425.1"
                     /translation="TQDEIDTARSARFAKYDANGDGKLGIDEFDALFREVTRPMMVRA
                     FQRLDPDGDAGVTTDEYDAAFSGMVERMDRNGDGALSRDDMRRHRRGHSDDDRNDDSD
                     EG"
     gene            complement(404..1129)
                     /locus_tag="VGB88_13060"
     CDS             complement(404..1129)
                     /locus_tag="VGB88_13060"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX9648426.1"
                     /translation="MRRRRLALALAATLAAVPVSRADEVLPIGPDLEALGWQVLTFSG
                     KAADRFVGHDDGSLEVISERSVSMLHRPVAVDLAAAPCLSWRWRVDEAPPPTNLTAHG
                     GDDRALAVFVAFPFDGEHASFWEQLVRPLVELFYGADAPGRVVAYVWGGRGQPGDMQS
                     SAYMGAAGRNVLLHPGDAPLGQWLEETVDVAADYRRAFDDPVTRPVRLAITSDTDDTR
                     SRAHAWIAALRFHAGCGQPAGAR"
     gene            complement(1126..1470)
                     /locus_tag="VGB88_13065"
     CDS             complement(1126..1470)
                     /locus_tag="VGB88_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648427.1"
                     /translation="MAEGGGRWALSLAEALALPVPAGRRSALALAHGTMSVRFYAPAG
                     RDEQTPHDQDEIYVVARGRGRFLRAGDRVPFAPGTVLFVPAGVEHRFEDFTDDFATWV
                     VFWGPDGGEGDG"
     gene            complement(1478..3046)
                     /gene="gcvPB"
                     /locus_tag="VGB88_13070"
     CDS             complement(1478..3046)
                     /gene="gcvPB"
                     /locus_tag="VGB88_13070"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9648428.1"
                     /translation="MITDRSQGRKTAERALRASANSGTVTGNRGLAIEEPLIFEQGAD
                     GRSGVDLPDPPAHAPRLGGLARTRACGLPGLSEPQVVRHYTRLSALNYGIDTGFYPLG
                     SCTMKHNPRLGERLAGLPGFADLHPLQPLGTVQGALELIDRLAEWLKTLCGLPAVAMS
                     PAAGAQGELCGMMVIRACLEARGDARQVVLVPESAHGTNPATAVACGYRIEEIPADDS
                     GRVDLASLSARLGPDVAAIMLTNPNTCGLFERDTLAIAEAVHAAGALFYCDGANFNAI
                     VGRVRPGDLGIDAMHLNLHKTFSTPHGGGGPGSGPVAMTAELARFAPLPWRLHGAGGF
                     GLREHAKGEAAAAIGRLRAFHGQFGVFVRALAYMMSHGADGLRQVAEDSVLAANYLLA
                     RLDGALTPAYPGPCMHEALFDDRFLKGTGVSTLDFAKALIDEGFHPPTIYFPLVVHGA
                     LLVEPTETETRETLDLFADTVRALAERAKAGDAAYFHDAPRHAPRRRLDETRAARQPV
                     LRWRPAAPDAAAAE"
     gene            complement(3043..>3397)
                     /locus_tag="VGB88_13075"
     CDS             complement(3043..>3397)
                     /locus_tag="VGB88_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEX9648429.1"
                     /translation="CALAFSIHLALLGDDGFARLARLNHAYAVRAADRLDEVPGVEVL
                     NETFFNEFTVRLARPAADVVEALAERGILAGVPVSRLYPGRDDLAPLLLVAVTETNTD
                     DDVEALARVLGEAVS"
BASE COUNT          471 a         1247 c         1269 g          410 t
ORIGIN      
        1 tcacccagga cgagatcgac acggcgcgca gcgcgcggtt cgccaagtac gacgccaacg
       61 gcgacggcaa gctcggcatc gacgagttcg atgccctgtt ccgcgaggtc acgcggccga
      121 tgatggtgcg ggccttccag aggctcgacc cggacggcga cgccggcgtc accaccgacg
      181 aatacgatgc cgccttctcg ggcatggtcg agcgcatgga ccgcaacggc gacggcgccc
      241 tgtcgcgcga cgacatgcgc cgccaccgcc gcgggcactc ggacgacgac cgcaacgacg
      301 acagcgacga gggctgactc cgattgtcct cgacgcctcc ggcggggctg aacggcgccc
      361 cgccggactc cccttcaata cctagcccgc tatcgatcgt ggctcatcgt gcgccggccg
      421 gctgcccgca gccggcgtgg aagcggagtg cggcgatcca cgcgtgcgcg cggctgcggg
      481 tgtcgtcggt gtccgaggtg atcgcgagcc gcaccggccg ggtgaccggg tcgtcgaagg
      541 cgcgccggta gtcggcggcg acatcgacgg tctcctcgag ccattgcccg agcggggcgt
      601 cgcccggatg gagcagcacg ttgcggccgg cggcgcccat ataggcgctg ctctgcatgt
      661 cgcccggctg gccgcggccg ccccagacat acgccaccac ccgccccggg gcgtcggcgc
      721 cgtagaacag ctcgaccagc gggcgcacca gctgctccca gaagctggcg tgctcgccgt
      781 cgaacgggaa cgccacgaac accgcgagcg cgcggtcgtc gccgccatgc gccgtgaggt
      841 tggtcggcgg cggcgcctcg tccacccgcc agcgccacga caggcacggc gccgccgcga
      901 ggtcgaccgc gaccggccgg tgcagcatcg agacgctgcg ctcgctgatc acctcgaggc
      961 tgccgtcgtc atggccgacg aagcggtccg ccgccttgcc ggagaaggtc agcacctgcc
     1021 agccgagcgc ctcgaggtcg gggccgatcg gcaggacctc gtccgcccgc gacacgggca
     1081 ccgccgccag cgtcgccgcc agcgccagcg ccagccggcg ccgcctcacc cgtcgccctc
     1141 gccgccgtcc gggccccaga acaccaccca ggtcgcgaag tcgtcggtga agtcctcgaa
     1201 ccggtgctcg acgcccgccg gcacgaacag caccgtgcct ggcgcaaacg ggacgcggtc
     1261 gccggcgcgc aggaatcggc cccggccgcg ggcgacgacg tagatctcgt cctggtcgtg
     1321 cggggtctgc tcgtcgcggc ccgcgggcgc gtagaagcgc accgacatgg tgccgtgggc
     1381 gagggcgagc gccgagcgcc ggccggccgg caccggcagc gccagcgcct cggccagcga
     1441 cagcgcccag cgaccgccgc cctcggccat cccgacccta ctccgcggcc gcggcgtccg
     1501 gcgcggccgg gcgccagcgc agcaccggct ggcgcgcggc gcgggtctca tccaggcgcc
     1561 ggcgcggggc gtggcggggg gcgtcgtgga agtacgcggc atcgcccgcc ttggcccgct
     1621 cggccagcgc gcgcacggtg tcggcaaaca ggtcgagagt ctcgcgggtc tcggtctcgg
     1681 tcggctcgac cagcagcgcg ccgtggacga ccagcgggaa gtagatggtc ggcgggtgga
     1741 agccctcgtc gatcagcgcc ttggcgaagt cgagggtcga tactccggtg cccttgagga
     1801 agcggtcgtc gaacagcgcc tcgtgcatgc acgggccggg gtaggccggg gtgagcgcgc
     1861 cgtcgaggcg ggcgagcagg tagttggccg ccagcaccga gtcctcggcc acctggcgca
     1921 ggccgtcggc gccgtggctc atcatgtagg cgagcgcgcg cacgaacacg ccgaactggc
     1981 cgtggaaggc gcgcagccgt ccgatcgccg ccgccgcctc gcccttggcg tgctcgcgca
     2041 ggccgaagcc gcccgcgccg tgcagccgcc agggcagcgg cgcgaagcgg gcgagctcgg
     2101 cggtcatcgc caccggcccc gaccccggcc cgccgccgcc gtggggggtg gagaaggtct
     2161 tgtgcaggtt gaggtgcatc gcgtcgatgc cgaggtcgcc cgggcgcacc cgaccgacga
     2221 tggcgttgaa gttggcgccg tcgcagtaga acagcgcgcc ggcggcgtgc accgcctcgg
     2281 caatggcgag ggtgtcgcgc tcgaacaggc cgcaggtgtt ggggttggtg agcatgatcg
     2341 cggcgacgtc ggggccgagc cgggcgctga gcgaggcgag gtcgacccgg ccgctgtcgt
     2401 cggccgggat ctcctcgatc cgatagccgc aggcgaccgc ggtcgcgggg ttggtgccgt
     2461 gcgcggactc gggcaccagt accacctggc gggcatcgcc gcgggcctcc aggcaggcgc
     2521 ggatcaccat catcccgcac agctcgccct gggcgccggc ggcgggcgac atcgccaccg
     2581 ccggcaggcc gcacagcgtc ttgagccact ccgccagccg gtcgatcagc tcgagtgcgc
     2641 cctgcacggt gccaagcggc tgcagcgggt gcaggtcggc gaagcccggc agcccggcga
     2701 ggcgctcgcc gagccgcgga ttgtgcttca tggtgcacga gccgagcggg tagaagccgg
     2761 tgtcgatgcc gtagttgagc gcgctgagcc gcgtgtagtg gcgcaccacc tgcggctcgg
     2821 acagcccggg caggccgcag gcgcgggtgc gcgcaagccc gccgagccga ggcgcgtgcg
     2881 ccggcggatc gggcagatcg acgccgctgc gcccgtccgc gccctgctcg aagatcagcg
     2941 gctcctcgat ggcgaggccg cggttgccgg tcaccgtgcc ggaattggca ctcgcgcgca
     3001 gcgcgcgctc ggccgtcttt cgtccctggc tcctgtccgt gatcatgaca ccgcctcccc
     3061 cagcacgcgc gccagcgcct cgacgtcgtc gtcggtgttg gtttcggtga ccgcgaccag
     3121 cagcagcggc gcaaggtcgt cgcgccccgg atagaggcgc gataccggca ccccggccag
     3181 gatgccgcgt tctgccagcg cctcgaccac atcggccgcg gggcgcgcaa ggcgcaccgt
     3241 gaactcgttg aagaaggtct cgttgagcac ctcgacgccc ggcacctcgt caagccggtc
     3301 ggcggcgcgc accgcgtagg cgtggttgag ccgggcgagg cgggcaaagc cgtcgtcgcc
     3361 cagcagcgcc aggtggatcg agaaggcgag cgcgcac
//
LOCUS       DASWHU010000353         7155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_598411, whole genome shotgun sequence.
ACCESSION   DASWHU010000353 DASWHU010000000
VERSION     DASWHU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598411"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGB88_13080"
     CDS             complement(<1..711)
                     /locus_tag="VGB88_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX9648430.1"
                     /translation="MQRVRVQFVAAEIFPLAKTGGLADVCAALPRALAERGVDVRLTM
                     PAYESALDQALAPVVAADLGEVLGVERVRLLRARAPDSGLPLWLVDAPALFRRSGGPY
                     QDAEGRDWPDNARRFAVFAHAAARIALGRAGLDWVPDVVHCHDWHAGLVPLLLRAAAE
                     RPPSLFTVHNMSFQGTFPESTLGGLGLPVAGLTDGVEFYGQLSFLKAGLSFADGLTTV
                     SETYAREIQTPEFGHGLDG"
     gene            927..2633
                     /gene="glgP"
                     /locus_tag="VGB88_13085"
     CDS             927..2633
                     /gene="glgP"
                     /locus_tag="VGB88_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX9648431.1"
                     /translation="MTPATIGPFLGRTRIAYFSMEIALQPDIPTYSGGLGVLAGDTAR
                     GCADLELPVVFVTLISRQGYLRQEIDAEGWQVEHPDPWQPAEHATPLRAKVAIPIAGR
                     EVWVRPWLFVLQSTLGYRVPVLLLDTDLDENTPEDRRITDVLYGGDEEYRLKQEMVLG
                     ICGVRILQALGFSIHRYHLNEGHAALLALDLLRRHPRPADQIARGELAYDVGQVRESC
                     IFTTHTPVEAGHDRFSYELARKLLPDYMPFDQLKLLTGNEHFNMTRLALNLSGYVNGV
                     ATRHAETTLRMFPGHRIHAVTNGVHLATWMHPRIAELFDRHLPLWRVEPEILARTDRL
                     DDDEVWDAHAAAKAEFLKLVADRTGCVMDPEVPVLGFARRMTGYKRPAFLFADLERLL
                     AVHQRTPFQVVIAGKAHPRDETGKHAIHIIHRRIGEMQGRMRAAFLPNYDMALAKHVV
                     AGVDIWLNTPVPPMEASGTSGMKAALNGALNLSVLDGWWVEGCIEGVTGWAISHDSGR
                     AAVEATETDLYDKLEHAVLPTYYHDRARWVWMMKQAMAKIGSYFNTNRMMRRYAAEAY
                     VV"
     gene            2673..5090
                     /gene="ppsA"
                     /locus_tag="VGB88_13090"
     CDS             2673..5090
                     /gene="ppsA"
                     /locus_tag="VGB88_13090"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX9648432.1"
                     /translation="MAKRDGLVIWFDDLKRTDVALVGGKNASLGEMVGSLGAEGVRVP
                     PGFATSADAYWQFVEANGLMGVIEAAMRDLDTGKHTLQEAGRAVRQAFVRGDWPEACA
                     RAIRAAYRVLSKRCGAAETDVAVRSSATAEDLPDASFAGQQETFLNVRGERALLDACR
                     RCYASLFTDRAISYRAAKGFDHLQVALSIGVQKMVRSDLAGAGVMFSIDTETGFDKVV
                     VINAAWGLGENVVQGTVNPDEYQVFKPLLADPGLTPIIERALGAKERKMIYAGGAAPT
                     RNVPTSRSERASFVLADAEILQLARWACAIEAHYGMAMDMEWAKDGEAGDLYIVQARP
                     ETVQSRKQGPNFRTYRMTRKGRQLVAGLSIGDAIVTGEVSLIPGADRIADFRDGAVLV
                     TATTDPDWVPIMKRAAAIVTDHGGRTSHAAIVSRELGLPAVVGTGDATRLLHDQQAVT
                     VSCAEGDQGFVYEGIGEFEVDELSLDDIPETRTQVMLNLANPAAAFRWWRLPADGVGL
                     ARMEFVVNNQIKVHPMALVRYDSLRDEDAKRRIGELTEGYADKTEYFVDRLARALARI
                     AAAHHPNPVIVRTSDFKTNEYANLIGGAEFEPKEQNPMIGFRGASRYTSPRYREGFAL
                     ECRALRRLREEMGFANVIVMIPFCRSTGEADRVLEVMAENGLERGVRGLEVYVMCEIP
                     SNVVLAKAFAERFDGFSIGSNDLTQLTLGVDRDSELLAGLFDEQDEAVEWMIRSVIEA
                     AHRAGAKIGLCGQAPSDHPEFAEFLVDCGIDSISVSPDSFIAVKQSVARAEARRAARA
                     KLSRSGA"
     gene            complement(5071..>7155)
                     /locus_tag="VGB88_13095"
     CDS             complement(5071..>7155)
                     /locus_tag="VGB88_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9648433.1"
                     /translation="GQGTGVVVATGDATEIGRIGTLLAGTESLTTPLLRQMAVFGRWL
                     TAGILGLAAVTFAVGTVGHGFTAGDMFLAAVGLAVAAIPEGLPAIMTVTLAIGVERMA
                     RRNAIIRRLPAVETLGSVSVICSDKTGTFTRNEMMVESIATSRHMFAVAGDGYAPAGE
                     FSLDGVPVDLAAHPRLETLARGAILCSDADLSRDGDAWRIEGDPMEAALVVVGMKANL
                     DPDVERDSWPRSDLIPFDSGHRFMATLHHDRSRRGVVFVKGAPESVLPMCSRQGEADG
                     AGPIDLAYWHGRMDEITRRGQRVLALAMKDTGPDHHSLGFADLGSGLTLLGLFGFGDP
                     PREEAIEAVAKCRAAGIRVKMITGDHAGTARAIAQKLGLRNALTVVTGTDLDRMDDEE
                     LAEHVIDVDVFARTTPEHKLRLVEALQARRRIVAMTGDGVNDAPALKRADVGIAMGRK
                     GSEAAKEAAEMVLADDNFASIAHAVEEGRTVYDNLKKAILFLLPTNGGEALTIVAAIA
                     LGRLLPITAVQILWINMITAVTLALALAFEPAEAEVMKRPPRDAREPILSGFVLWRIA
                     LVSLVLVAGVFGIFVWLRDSGADIETARTAAVNTLVLFEAFYLFNVRRMQSSAFASLF
                     DRTAWIAWAAVATVVSFQLLFTYAPFMQTLFQSRPLAAYVWLAVLGVAAIIFLIVELE
                     KTLLRALRRRTG"
BASE COUNT         1147 a         2522 c         2472 g         1014 t
ORIGIN      
        1 gccgtcgagg ccatggccga actctggcgt ctggatctcg cgcgcatagg tctcgctgac
       61 cgtggtcagg ccgtcggcaa aggacagccc ggccttgagg aacgacagct ggccgtagaa
      121 ctcgaccccg tccgtgaggc ccgccaccgg cagcccaagg ccgccgagcg tggattcggg
      181 aaaggtgccc tggaatgaca tgttgtggac cgtgaacagg cttggcgggc gttctgccgc
      241 ggcgcgcagg agcagcggca ccagcccggc gtgccagtcg tggcagtgga ccacgtccgg
      301 gacccagtcg aggccggctc gcccgagcgc gatccgcgcc gcggcatggg cgaagacggc
      361 gaaccgcctg gcattgtcgg gccaatcgcg cccctcggcg tcctggtagg gcccgccgga
      421 gcgccggaac agcgccggcg cgtcgaccag ccacagcggc aggccgctgt ccggggcgcg
      481 cgcgcgcagc aggcgcacgc gctcgacgcc gagcacctcg ccgaggtccg cggccaccac
      541 cggcgcgagc gcctggtcga gcgccgattc gtaggccggc atggtcaggc ggacgtcgac
      601 gccgcgctcg gccagcgccc gcggcagcgc agcgcagacg tcggccaggc cgccggtctt
      661 tgctagaggg aatatctcgg ccgctacgaa ctggacccgc acccgttgca ccttggcact
      721 cggtgaccgc ttggctcccg acggagcccc cctccccctc ggcaacggcg ccaagatggt
      781 acaaatacac ttaggggcct tgacatggat cagccactgg ctttcggtct catgaatttt
      841 tcgtgtccct agatgttgta gctgaagaca agcgggcact cgatcagtag cggggggcga
      901 gcgtgacgcc actcaagaaa gcggcggtga ctccggcgac catcggcccg tttctcggcc
      961 ggacccgaat cgcctacttc tcgatggaga tcgcgctcca gcccgacatc ccgacctaca
     1021 gcggcgggct gggggtgctg gccggcgaca ccgcgcgcgg ctgcgccgac cttgagctgc
     1081 cggtggtgtt cgtcaccctg atcagccgcc aaggctacct gcgccaggag atcgacgccg
     1141 aaggctggca ggtggagcac ccggacccgt ggcagccggc cgagcacgcc accccgctca
     1201 gggccaaggt cgcgataccg atcgccgggc gcgaggtgtg ggtgcggccg tggctgttcg
     1261 tgctgcagag cacgctcggc taccgcgtgc cggtgctgct gctggatacc gacctcgacg
     1321 agaacacgcc cgaggaccgg cgcattaccg acgtccttta cggcggcgac gaggagtacc
     1381 gcctgaagca ggagatggtg ctgggcatct gcggggtgcg catcctgcag gcgctcggct
     1441 tctcgatcca ccgctatcac ctgaacgagg gccatgccgc gctgctcgcc ctcgacctgc
     1501 tgcgccgcca cccccgcccg gccgaccaga tcgcccgcgg cgagctcgcc tacgacgtgg
     1561 gccaggtgcg cgaatcctgc atcttcacca cccacacccc ggtcgaggcc ggccacgacc
     1621 gcttctccta cgagctggcg cgcaagctgc tgccggacta catgccgttc gaccagctca
     1681 agctgctgac cggcaacgag catttcaaca tgacccggct cgcgctcaac ttgagcggct
     1741 atgtcaacgg ggtcgccacg cgccacgccg agaccacctt gcgcatgttc cccggccacc
     1801 gcatccacgc cgtgaccaac ggcgtgcatc tggcgacctg gatgcacccg cgcatcgccg
     1861 agctgttcga tcggcacctg ccgctgtggc gggtcgagcc cgagatcctg gcccgcaccg
     1921 accggctcga cgacgacgag gtctgggacg cccacgccgc cgccaaggcc gagttcctga
     1981 agctggtcgc ggaccggacc ggctgcgtca tggacccgga ggtcccggtg ctcggtttcg
     2041 cccgccgcat gaccggctac aagcgcccgg cgttcctgtt cgccgacctc gagcgcctgc
     2101 tcgccgtcca ccagcgcacg ccgttccagg tcgtgatcgc cggcaaggcg catccgcgcg
     2161 atgagaccgg caagcacgcg atccacatca tccaccgccg gatcggcgag atgcagggcc
     2221 gcatgcgcgc cgcgttcctg cccaactacg acatggcgct ggccaagcac gtggtcgccg
     2281 gcgtcgacat ctggctcaac accccggtgc cgccgatgga ggcctcgggc accagcggca
     2341 tgaaggccgc gctcaacggc gcgctcaact tgagcgtgct cgacggctgg tgggtcgagg
     2401 gttgcatcga aggggtcacc ggctgggcga tcagccacga cagcggcagg gcggccgtcg
     2461 aggcgaccga gaccgacctc tacgacaagc tcgagcacgc ggtgctgccg acctactacc
     2521 acgaccgcgc gcggtgggtg tggatgatga agcaggcgat ggccaagatc ggcagctact
     2581 tcaataccaa tcgcatgatg cgccgctacg ccgcggaagc ctacgtggtc tagccgtggg
     2641 aaccggcgcc ggcgcctgga agcgggagag agatggcgaa gcgcgacggg ctggtgatct
     2701 ggttcgacga cctcaagcgc accgacgtcg ccctggtcgg cggcaagaac gcctcgctgg
     2761 gcgagatggt gggcagcctc ggcgccgaag gcgtgcgggt gccgccggga ttcgccacca
     2821 gcgccgacgc ctactggcag ttcgtcgagg cgaacggcct catgggcgtg atcgaggcgg
     2881 caatgcgcga cctcgacacc ggcaagcata cgctgcagga ggcgggccgg gcggtgcgcc
     2941 aggcgttcgt tcgcggcgac tggcccgagg cgtgcgcgcg cgcgatccgc gccgcctacc
     3001 gcgtgctctc gaagcgctgc ggggcggctg agaccgacgt cgcggtgcgc tcgagcgcga
     3061 ccgccgagga cctgcccgac gcgagcttcg ccggccagca ggagaccttc ctcaacgtcc
     3121 gtggcgagcg cgcgctgctc gatgcctgcc ggcgctgcta cgcctcactg ttcaccgacc
     3181 gggcgatcag ctatcgcgcg gccaagggct tcgaccacct gcaggtggcg ctgtcgatcg
     3241 gggtccagaa gatggtccgc tccgacctcg ccggcgccgg ggtcatgttc tcgatcgaca
     3301 ccgagaccgg cttcgacaag gtggtggtga tcaacgccgc gtgggggctc ggcgagaacg
     3361 tggtgcaggg gacggtgaat cccgacgagt accaggtgtt caagccgctg ctcgccgacc
     3421 cgggcctgac gccgatcatc gagagggcgc tcggcgccaa ggagcgcaag atgatctacg
     3481 cgggcggcgc ggcgccgacc cgcaacgtgc cgacgtcgcg ctccgagcgc gcctcgttcg
     3541 tgctcgccga tgccgagatc ctgcagctcg cgcgctgggc gtgcgcgatc gaggcgcatt
     3601 acggcatggc gatggacatg gagtgggcga aggacggcga ggccggcgat ctctacatcg
     3661 tccaggcgcg ccccgagacc gtgcagtcgc gcaagcaggg ccccaacttc aggacctacc
     3721 gcatgacgcg gaagggccgc cagctggtcg ccggcctcag catcggcgac gccatcgtca
     3781 ccggcgaggt gtcgctgata ccgggcgcgg accgcatcgc cgacttccgc gacggcgcgg
     3841 tactggtcac cgcgaccacg gacccggact gggtgccgat catgaagcgc gccgccgcca
     3901 tcgtcaccga ccacggcggg cgcaccagcc acgccgccat cgtcagccgc gagctcggcc
     3961 tgccggcggt ggtcggcacc ggcgatgcca cccgcctgct ccatgaccag caggcggtca
     4021 ccgtgtcctg cgccgagggc gaccaaggct tcgtctacga gggcattggc gagttcgagg
     4081 tcgacgagct cagcctcgac gacatccccg agacgcgcac gcaggtgatg ctcaacctcg
     4141 ccaacccggc ggccgcgttc cgctggtggc gcctgccggc cgacggcgtc ggccttgcgc
     4201 gcatggaatt cgtggtcaac aaccagatca aggtccaccc gatggcgctg gtccgctacg
     4261 acagcttgcg cgatgaggac gccaagcgcc gcatcggtga gctcaccgaa ggctacgccg
     4321 acaagaccga gtatttcgtc gaccgcctgg cgcgcgcgct ggcgcggatt gcggcggcgc
     4381 accaccccaa tccggtgatc gtgcgcacca gcgacttcaa gaccaacgag tacgccaacc
     4441 tgatcggcgg cgccgagttc gagcccaagg agcagaaccc gatgatcggc ttccgcggcg
     4501 cctcgcgcta cacctcgccg cgctaccgcg aagggtttgc gctcgaatgc cgggcgctgc
     4561 gccggctgcg cgaggagatg gggttcgcca acgtgatcgt gatgatcccg ttctgccgct
     4621 cgacgggcga ggccgaccgg gtgctcgagg tcatggccga gaacggcctc gaacgcgggg
     4681 tgcgggggct tgaggtctac gtgatgtgcg agatcccgtc caacgtggtg ctggccaagg
     4741 cgttcgccga gcgcttcgac ggcttctcga tcggctccaa cgacctcacc cagctcaccc
     4801 tgggggtcga ccgcgattcc gagctgctgg cgggcctgtt cgacgagcag gacgaggcgg
     4861 tcgagtggat gatccgctcg gtgatcgagg ccgcgcaccg cgccggcgcc aagatcgggc
     4921 tgtgcggcca ggcgccgagc gaccaccccg agttcgccga gttcctggtc gactgcggca
     4981 tcgactcgat ctcggtcagc cccgacagct tcatcgccgt caagcagtcc gtcgcccgcg
     5041 ccgaggcgcg gcgcgcggcg cgcgccaagc tcagccggtc cggcgcctga gcgcgcgcaa
     5101 cagcgtcttc tccagctcga cgatcaggaa gatgatggcc gcgacgccaa gaacggcgag
     5161 ccagacataa gccgccagcg gccgcgactg gaacagcgtc tgcatgaacg gcgcataggt
     5221 gaacagcagc tggaacgaga cgacggttgc cacggccgcc cacgcgatcc acgcggttcg
     5281 gtcgaacagg ctggcgaatg ccgacgactg catccgccgc acgttgaaca ggtagaacgc
     5341 ctcgaacagg accagcgtgt tgaccgcggc agtgcgcgcg gtctcgatgt cggcgcccga
     5401 gtccctgagc cagacgaaga tgccgaacac gcccgcgacc agaaccagcg acacgagcgc
     5461 gatccgccac agcacgaacc cggacaggat cggctcgcgc gcatcccgcg gcgggcgttt
     5521 catcacctcg gcctcggcgg gctcgaaggc gagcgccagc gccaaggtca ccgccgttat
     5581 catgttgatc cacaggatct ggacggcggt gatcggcagc agccggccga gcgcgatcgc
     5641 ggcaacgatg gtcagcgcct cgccgccgtt ggtcggcagg aggaacagga tcgccttctt
     5701 caggttgtcg tagaccgtgc gtccctcctc gaccgcatgt gcgatcgagg cgaagttgtc
     5761 gtcggccagc accatctcgg ccgcctcctt ggccgcctcg gagcccttgc ggcccatggc
     5821 gatgccgacg tcggcgcgct tcagcgccgg cgcgtcgttg accccgtcgc cggtcatggc
     5881 gacgatccgc cggcgcgcct ggagcgcctc gaccagcctg agcttgtgct ccggggtggt
     5941 gcgcgcgaac acgtcgacgt cgatcacgtg ctccgcgagt tcctcgtcgt ccatgcggtc
     6001 gaggtcggtg ccggtgacca ccgtaagcgc gttgcgcagg ccgagcttct gggcaatcgc
     6061 ccgcgccgtg ccggcatgat cgccggtgat catcttgacc cggatgccag ccgcgcggca
     6121 tttcgcgacc gcctcgatcg cctcctcgcg cggcggatcg ccgaacccga aaagaccaag
     6181 caaggtgaga ccggagccga ggtcggcgaa gccgagcgaa tgatgatccg ggccggtgtc
     6241 cttcatcgcc agcgccagca ccctctggcc gcggcgcgtg atctcgtcca tccggccatg
     6301 ccagtaggcg aggtcgatcg ggcctgcgcc gtcggcctcg ccttgccggc tgcacatcgg
     6361 cagcacgctt tccggcgcgc ccttgacgaa caccacgccc cggcgcgagc ggtcgtggtg
     6421 gagcgtggcc atgaacctgt gaccggagtc gaacgggatc aggtcgctgc gcggccatga
     6481 gtcgcgctcg acgtcgggat cgaggttcgc cttcatgccg accacgacca gggccgcttc
     6541 catcgggtcg ccctcgatgc gccacgcgtc cccgtcgcgg gagaggtccg cgtcgctgca
     6601 caggatcgcg cctcgcgcca gggtttcaag gcgcgggtgc gccgcgaggt cgaccgggac
     6661 gccgtcgagc gagaactcgc ccgccggggc atagccgtcc ccggcgaccg cgaacatatg
     6721 gcggctggtg gcgatgctct cgaccatcat ctcgttgcgc gtgaacgtgc cggtcttgtc
     6781 ggagcagatg acgctgaccg agcccagcgt ctcgaccgcg ggcaggcggc ggatgatggc
     6841 attgcgccgg gccatgcgct cgacgccgat ggcgagcgtg acggtcatga tcgcgggcaa
     6901 tccctccggg atcgcggcga ccgcgagccc gactgcggcg aggaacatgt cgcccgcggt
     6961 aaatccgtgg ccgacggtgc cgacggcgaa ggtcaccgcc gccagcccca ggatgcccgc
     7021 ggtgagccag cggccgaaga cggccatctg gcgcaacaaa ggcgtggtca ggctctcggt
     7081 gcccgcgagc agcgtgccga tccggccgat ctcggtcgca tcgccggtgg cgaccacgac
     7141 gccggtcccc tggcc
//
LOCUS       DASWHU010000354         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3201692, whole genome shotgun sequence.
ACCESSION   DASWHU010000354 DASWHU010000000
VERSION     DASWHU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3201692"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /gene="acs"
                     /locus_tag="VGB88_13100"
     CDS             complement(<1..1451)
                     /gene="acs"
                     /locus_tag="VGB88_13100"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9648434.1"
                     /translation="MSDNLVFPVPKGLAKSALIDQAGYERMYRQSIEDPDAFWAEQAK
                     RIDWVKAPTRIKNTSFKRPNVSIKWFEDGVLNVSANCVDRHAARTPDRIAILWEGDDP
                     KVDRAVTYGELKREVCRMANVLKKHGIGKGDRVTIYLPMIPEIAFAMLACARIGAIHS
                     VVFGGFSPDSLADRIHDCDSAMVITADEGLRGGRKVPLKANADAALERCPSVKSVLVV
                     RRTGAGIAWSKGRDLWWHEERATVADDCAPEPMGAEDPLFILYTSGSTGQPKGVLHTT
                     GGYLVYVALSHELIFDYHAADIFWCTADVGWVTGHSYIVYGPLCNGATSLMFEGVPNY
                     PDASRFWQVVDKHKVTIFYTAPTAIRALMREGDGPVRKTSRKSLRILGTVGEPINPEA
                     WLWYYNVVGDARCPIVDTWWQTETGGILITPLPGAIALKPGSATKPFFGVQPVIVDGD
                     GKRLDGPAVGNLCMADAWPGLMRTVYGDHERFVA"
     gene            complement(1544..1918)
                     /locus_tag="VGB88_13105"
     CDS             complement(1544..1918)
                     /locus_tag="VGB88_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX9648435.1"
                     /translation="MAEAVRLCALDDIADGAARLFETEPDRAPDIFVVRKGARAFCYV
                     NDCPHQHLPLDFVPGRFLDLSRRHILCTNHGAWFEIETGACLRGPCRGERLCALAVRI
                     EHGAVWLDGGAPVRVKPGQGPG"
     gene            complement(1921..2334)
                     /locus_tag="VGB88_13110"
     CDS             complement(1921..2334)
                     /locus_tag="VGB88_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9648436.1"
                     /translation="MAYWLFKSEPGSYAWADLVREGRTSWDGVRNHQASNNMKAMAKG
                     ERGFFYHSGEVRAIVGVVEVARAYYPDHTDASGRFGMVDVTPVKPVPRPVALAEIKAE
                     PKLSDLLLVRNSRLSVVPVGAAHWRVLCRMAGIEA"
BASE COUNT          355 a          819 c          812 g          357 t
ORIGIN      
        1 gcgacgaagc gctcgtggtc gccgtagacg gtgcgcatca gccccggcca ggcgtcggcc
       61 atgcacaggt tgcccaccgc cgggccgtcg agccgcttgc cgtcgccgtc gacgatcacc
      121 ggctggacgc cgaagaacgg cttggtcgcc gagcccggct tgagcgcgat cgcgcccggc
      181 aggggcgtga tcaggatgcc gccggtctcg gtctgccacc aggtgtcgac gatcgggcag
      241 cgggcgtcgc cgaccacgtt atagtaccac agccaggcct cgggatttat cggctcgccg
      301 accgtgccga ggatgcgcaa ggacttgcgg ctggtcttcc tgaccgggcc gtcgccttcg
      361 cgcatcagcg cccggatcgc ggtcggcgcg gtgtagaaga tggtcacctt gtgcttgtcc
      421 accacctgcc agaagcgcga ggcgtcgggg tagttgggca cgccctcgaa catcagcgag
      481 gtggcgccgt tgcacagcgg gccgtagacg atgtagctgt ggccggtgac ccagccgacg
      541 tcggcggtgc accagaagat gtcggcggcg tggtagtcga agatcagctc gtggctcagc
      601 gcgacgtaga ccaggtagcc gccggtggtg tgcagcacgc ccttgggctg gccggtcgag
      661 cccgaggtgt agaggatgaa cagcgggtcc tcggcgccca tcggctccgg cgcgcagtcg
      721 tccgcgaccg tagcgcgctc ctcgtgccac cacaggtcgc ggcccttgct ccaggcgatc
      781 cccgcgccgg tgcgccgcac caccagcacg ctcttgaccg acgggcagcg ctcgagcgcc
      841 gcgtcggcgt tcgccttcag cggcaccttg cgcccgccgc gcaggccctc gtcggcagtg
      901 atcaccatcg ccgagtcgca gtcgtggatg cggtcggcga gggaatcggg cgagaagccg
      961 ccgaacacca ccgaatggat cgcgccgatg cgcgcgcagg cgagcatggc gaatgcgatc
     1021 tcggggatca tgggcagata gatggtcacc cggtcgccct tgccgatgcc gtgcttcttg
     1081 agcacgttcg ccatccggca cacctcgcgt ttaagctcgc cataggtgac ggcgcggtcg
     1141 accttggggt cgtcgccctc ccacaggatg gcgatgcggt cgggagtgcg cgcggcatgg
     1201 cggtcgacgc agttggccga gacgttgagc acgccgtcct cgaaccactt gatcgacacg
     1261 ttcgggcgct tgaacgaggt gttcttgatc ctggtcggcg ccttgaccca gtcgatccgc
     1321 ttcgcctgct cggcccagaa cgcgtcgggg tcctcgatcg actgccggta catgcgctcg
     1381 tagcccgcct ggtcgataag ggccgacttg gcaagcccct tgggcaccgg aaacaccagg
     1441 ttgtccgaca tcatccgctc cctgtctcgg ttgcagcgcg ctcgcgtcgg gcgggattaa
     1501 atcggcaaac ggccgccgcg taaacggcag aggcggcgga atctcagccc ggcccctggc
     1561 cgggcttgac gcgcacgggc gcgcccccgt cgagccacac cgccccgtgc tcgattcgca
     1621 ccgccagcgc gcacagccgc tcgccgcggc acggcccgcg caggcacgcg ccggtctcga
     1681 tctcgaacca ggcgccgtgg ttggtgcaca ggatgtggcg gcgggagagg tcgaggaagc
     1741 gcccgggcac gaagtcgagc ggcaggtgct ggtgcgggca gtcgttgacg tagcagaagg
     1801 cgcgcgcgcc cttgcgcacg acgaagatgt cgggggcgcg gtcgggctcg gtctcgaaca
     1861 ggcgcgcagc cccgtcggcg atgtcgtcga gcgcgcacag cctgaccgcc tcggccatcg
     1921 tcaggcctcg atgccggcca tcctgcacag cacgcgccag tgggccgcgc ccacgggcac
     1981 caccgacagg cgcgagttgc gcaccagcaa caggtcggaa agctttggct cggccttgat
     2041 ctcggccagg gcgaccgggc gcgggaccgg cttgaccggg gtcacgtcga ccatgccgaa
     2101 gcgcccgctt gcgtcggtgt ggtccgggta ataggcccgc gccacctcga ccacgccgac
     2161 gatcgcgcgc acctcgcccg agtggtaaaa gaacccgcgc tcgcccttgg ccatcgcctt
     2221 catgttgttc gacgcctggt ggttgcgcac cccgtcccag gaggtgcgcc cctcgcgcac
     2281 gagatccgcc cacgcatacg accccggctc cgacttgaac aaccagtacg ccatcgccgc
     2341 ctc
//
LOCUS       DASWHU010000355         7597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3539877, whole genome shotgun sequence.
ACCESSION   DASWHU010000355 DASWHU010000000
VERSION     DASWHU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539877"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGB88_13115"
     CDS             complement(<1..329)
                     /locus_tag="VGB88_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9648437.1"
                     /translation="MKLTLGWLKEHLDTGASLAEIADALTMLGLEVEQVIDRAAELAP
                     FTVAYVEAAAPHPNADRLKLCKVNTGTEVLEVVCGAPNARAGMKGVFAPLGARLPGTG
                     LTLEKRA"
     gene            complement(326..1429)
                     /gene="pheS"
                     /locus_tag="VGB88_13120"
     CDS             complement(326..1429)
                     /gene="pheS"
                     /locus_tag="VGB88_13120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9648438.1"
                     /translation="MDKDVKRTAERVRAEFLAAIADAVDLASLDQVRVAALGRKGRVS
                     ELMRALGAMAETERKRAGPLLNALKDDLGEALEGRRATLEEAALEAGLAEEGVDVTLP
                     ARPEPTGTVHPVSQVLDEVTEIFADMGFAVAEGPDIETDFHNFTALNIPPEHPARQMH
                     DTFYLPAKADGSQLLLRTHTSPVQIRTMMASEPPHKIIIPGRTYRRDLDMTHTPMFHQ
                     IEALVVDRTTHMGHLKGCVIDFARAFFEVADLPLRFRPSYFPFTEPSAEVDIGCTTQG
                     GEFRVGHLGAPEDGWMEILGCGMVHPRVLETCGLDAGEFQGFAFGMGIDRIAMLKYGI
                     RDLRGFFDCDARWLRHYGFRALEVPTLGRGLSR"
     gene            complement(1543..1899)
                     /gene="rplT"
                     /locus_tag="VGB88_13125"
     CDS             complement(1543..1899)
                     /gene="rplT"
                     /locus_tag="VGB88_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008553032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9648439.1"
                     /translation="MARVKRGVTTHARHKKAVKAAKGYSGRRKNVFRVAIQAVERARQ
                     YAYRDRRNRKRQFRALWIQRINAGAREHGLTYGQLIHGLAEAGVELDRKVLADIAARD
                     PASFKELAEAARAALA"
     gene            complement(1924..2124)
                     /gene="rpmI"
                     /locus_tag="VGB88_13130"
     CDS             complement(1924..2124)
                     /gene="rpmI"
                     /locus_tag="VGB88_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9648440.1"
                     /translation="MPKIKTKSGAKKRFRLTATGKVRMNHAGKRHGMIKRTNKFIRSM
                     RGTDIMAEADARIVRKHFLPNG"
     gene            complement(2430..3203)
                     /locus_tag="VGB88_13135"
     CDS             complement(2430..3203)
                     /locus_tag="VGB88_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9648441.1"
                     /translation="MRDNRVKDRLLQGEAVFGTMVFEFVAPGLPAVLAGAGAEFVIYD
                     MEHSGISEAEFKRQVSYCRGLSIVPMVRPPEKTYAATARLLDVGAMGLIFQMVESKSE
                     AEDIVSWTRYPPEGVRGAMFGGAHDDYAGGEVADKMAAANARTLVMALIETARGVEQV
                     DEILSVPGIDVAHIGHFDLSLTMGIPGKFDHPDFVAAIDTVLAACRKHEKPAGFLAPN
                     VDWGRAWMARGFRMVSYSYDIGLLGGALSAGISALKGTN"
     gene            3323..4495
                     /locus_tag="VGB88_13140"
     CDS             3323..4495
                     /locus_tag="VGB88_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9648442.1"
                     /translation="MTAPQPAMIAQPRLLMIGAGAAAEVAAAMAKIGVARPLIVTDPY
                     VRDSGMLARVTEVLDGAGVPYALFADTVPDPTTEVVEAGVAALADGDFDCLIAIGGGS
                     PIDTAKAMAVLASHGGRMRDYKVPNQVDATDYPLIAIPTTAGTGSEVTRFTVITDTET
                     DEKMLCAGLGLVPVCALIDYTLTLSMPWRLTADTGIDTLTHAIEAYVSRKQNPFSDAM
                     ARSCMRLVASHIRTACKEPGNGGAREAMMLAATQGGMAFSNASVCLVHGMSRPIGAHF
                     HVPHGLSNAMLLPAVTAFSAGHALDRYADCARAMGIVDGEVGNQAAVAVLLDELRALN
                     AELEVPTPKGYGIDERAYFELLPKMAEQALASGSPGNNPRVPDAGEIMDLYREAWA"
     gene            4504..5961
                     /gene="gndA"
                     /locus_tag="VGB88_13145"
     CDS             4504..5961
                     /gene="gndA"
                     /locus_tag="VGB88_13145"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX9648443.1"
                     /translation="MAGGPADPRSEAGAGIGVVGLGVMGANLALNLADHGAAVAVYNR
                     SAERAEAFLAGEAARQRAVSGHRSLESLIAALARPRTVLVMVQAGAAVDAQIDALTPL
                     LDAGDLVIDGGNAHYADTARRAEALAAAGLAFLGIGVSGGEVGARWGPAIMAGGDEAG
                     YARAAPLLEAIAAKVDGDPCCAWLGPGGAGHYVKMVHNGIEYALMQLIGEAWLMMRDG
                     LGLAPEAIAAAFAGWADGRLGGYLVEITADILAAPDAETGGMLLDVIDDRAGHKGTGR
                     WCAESALALGVPAATLAAGTLARSLSGASSARSLARRRLDGPETRVDDDIEAALADLE
                     QALLAGFVTAFSEGFQLLDAASAQFAWSLDRARIAALWRGGCIIRARLLDDIRTALSA
                     APGTPLLAAEPAFAAALAEAQPGWRRTVARAVRHGLPAPAMASALAAYDSYRTERLWT
                     ALVQAQRDYFGAHGYRRIDRVGDVHTDWATLAGDG"
     gene            5954..6490
                     /locus_tag="VGB88_13150"
     CDS             5954..6490
                     /locus_tag="VGB88_13150"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX9648444.1"
                     /translation="MADTAGLPVVVVVMGVAGSGKTTVGQRLADALGAAFEEGDDYHP
                     AANVEKMRGGVPLTDADRAPWLRRLAAGIDLWRDQGRRTVLACSALKESYREVLIGRR
                     HDVALVHLIGPEALIRMRLEARVGHYMPAQLLASQLASLEPPGDAITVDVAPAPERIV
                     EAILGRLGAWQRQSLQEE"
     gene            6494..7270
                     /locus_tag="VGB88_13155"
     CDS             6494..7270
                     /locus_tag="VGB88_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016434800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9648445.1"
                     /translation="MDLHLAGRTALVTGGSKGIGRAVAFGLAAEGCHLHLAARGEEAL
                     AKTRDEIARRHQVSVTTHAMDLSDSAAQGELVGRCDGVDILVNNAGGIPGGTLAQIDE
                     ARWREAWDLKVFGYVNLCRAFHARMCARGAGVIVNVIGLAGERFDAGYIAGSTANAGL
                     MAFTRALGSTSLENGVRVVGVNPGLVETDRMLFLMRARAQAELGNPDRWPELTKNLPL
                     GRAARADEVASLVTFLASDRASYVSGVVMTIDGGVAARAR"
     gene            complement(7413..>7597)
                     /locus_tag="VGB88_13160"
     CDS             complement(7413..>7597)
                     /locus_tag="VGB88_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9648446.1"
                     /translation="DDVDAMIARRNAQCPMGFMGDAWDTARAALFLASDEARYITGIE
                     LVVDGGLTLAMSPAQG"
BASE COUNT         1032 a         2704 c         2752 g         1109 t
ORIGIN      
        1 atcgcgcgct tctcgagcgt cagcccggtc cccggcaggc gcgcgccgag cggcgcgaac
       61 acgcccttca tgccggcgcg cgcattcggc gcgccgcaca ccacctcgag cacctcggtg
      121 ccggtattca ccttgcacag cttgagccgg tcggcgttgg ggtgcggcgc ggcggcctcg
      181 acataggcga ccgtgaacgg cgccagctcg gcggcgcggt cgatcacctg ctcgacctcg
      241 aggccgagca tggtcagcgc gtcggcgatc tcggccagcg aggcgccggt gtccagatgc
      301 tccttgagcc agccgagggt gagcttcatc ggctgaggcc ccggcccaag gtcggcacct
      361 cgagcgcgcg gaagccgtaa tggcgcagcc agcgcgcatc gcagtcgaag aagccgcgca
      421 ggtcgcggat gccgtactta agcatggcga tgcggtcgat gcccatgccg aaggcgaagc
      481 cctggaactc gccggcgtcc agcccgcagg tctcgagcac gcgcggatgc accatgccgc
      541 agcccaggat ctccatccag ccgtcctcgg gcgcgccgag atggccgacg cggaactcgc
      601 cgccctgcgt ggtgcagccg atgtcgacct cggccgaggg ctcggtgaac gggaagtagc
      661 tcgggcggaa gcgcagcggc aggtcggcga cctcgaagaa cgcgcgcgcg aagtcgatca
      721 cgcagccctt gaggtggccc atatgggtgg tgcggtcgac caccagcgcc tcgatctgat
      781 ggaacatggg ggtgtgggtc atgtcgaggt cgcggcggta ggtgcgcccc gggatgatga
      841 tcttgtgcgg cggctcgctc gccatcatgg tccggatctg caccggcgag gtgtgggtac
      901 gcagcaacag ctggctgccg tccgccttgg cgggcaggta gaaggtgtcg tgcatctggc
      961 gcgccggatg ctcgggcggg atgttgagcg cggtgaagtt gtggaagtcg gtctcgatgt
     1021 ccggcccctc ggcgaccgcg aagcccatgt cggcgaagat ctcggtgacc tcgtcgagca
     1081 cctggctgac cgggtgcacg gtgccggtgg gctccgggcg cgcggggagc gtcacgtcga
     1141 ccccttcctc ggcgagcccg gcctcgagcg ccgcctcctc gagcgtggcg cggcgcccct
     1201 ccagcgcctc gccgaggtcg tccttgagcg cgttcaagag tgggcctgcg cgcttgcgct
     1261 cggtctcggc catcgcgccg agggcgcgca tcagctcgct cacccgcccc ttgcggccga
     1321 gcgccgcgac tcgcacctgg tcgaggctgg cgaggtcgac ggcgtcggcg atcgcggcca
     1381 ggaactcggc gcggacgcgc tcggccgtcc gtttgacgtc cttatccatc gacggctctc
     1441 ataccagatt ccggcgcccc ggcggcaacc gccggtgcgg ccgcgcgggc cgatccgtca
     1501 ggacttgccc ggggcggtcc gcattttgcc ggccgggcgg gatcaggcga gcgcggcgcg
     1561 ggcggcctcg gccagctcct tgaacgaggc cggatcgcgc gcggcgatgt cggccagcac
     1621 cttgcggtcg agctcgaccc cggcctcggc caggccgtgg atgagctggc cataggtcag
     1681 cccgtgctcg cgcgcgccgg cgttgatgcg ctggatccac agcgcccgga actggcgctt
     1741 gcggttgcgc cggtcgcggt aggcgtactg gcgcgcccgc tcgaccgcct ggatggcgac
     1801 ccggaacacg ttcttgcgcc gcccgctata gcccttggcc gccttgaccg ccttcttgtg
     1861 gcgggcatgg gtggtgacgc cgcgcttgac ccgtgccatg acgcgctccc gtggtgccgg
     1921 cgctcagccg ttgggcagga agtgcttgcg cacgatgcgc gcgtccgctt cggccatgat
     1981 gtcggtgccg cgcatgctgc ggatgaactt gttggtgcgc ttgatcatgc cgtggcgctt
     2041 gccggcgtgg ttcatgcgca ccttgccggt cgcggtcagg cggaagcgct tcttggcgcc
     2101 gctcttggtc ttgatcttgg gcatggcgtg gatcctcgag cgcctgcgtc cgccaagcgc
     2161 ggaaagcgtg ccttatatcg acagccgccg cgcctggcaa gcgggccggc gcgggcgggc
     2221 gggggctgga gcgggatgat attacgtcgg atccctgagc ctgttttggt cgtcaccccg
     2281 gactccgcga agcggatatc cggggtccat acgcctttgc cacgcactcg accacccgtg
     2341 caagcggcgg gatggacccc ggctcgtggc gctgcgcgcc ttggccgggg tgacgggtga
     2401 tggatgacca tgtctcaaat catcaggctc tagttcgtgc ccttgagcgc ggaaatgccg
     2461 gcgctgagcg cgccgccgag caggccgatg tcgtacgaat aggagaccat gcggaagccg
     2521 cgcgccatcc aggcgcggcc ccagtcgacg ttcggcgcga ggaacccggc cggcttctcg
     2581 tgcttgcggc aggcggcgag cacggtatcg atcgcggcga cgaagtcggg atggtcgaac
     2641 ttgcccggga tgcccatggt cagcgacagg tcgaaatggc cgatatgggc gacgtcgatt
     2701 ccgggcaccg acaggatctc gtcgacctgc tcgaccccgc gcgcggtctc gatcagcgcc
     2761 atcaccagcg tgcgcgcgtt ggccgccgcc atcttgtcgg cgacctcgcc gccggcgtaa
     2821 tcgtcgtggg cgccgccgaa catcgccccg cgcacgccct cgggcgggta gcgggtccag
     2881 gagacgatgt cctcggcctc agacttgctc tcgaccatct ggaagatcag ccccatggcg
     2941 ccgacgtcca atagcctggc ggtggcggcg taggtcttct cgggcgggcg caccatcggc
     3001 acgatgctga gcccgcggca gtacgacacc tggcgcttga actcggcctc cgagatgccg
     3061 ctgtgctcca tgtcgtagat cacgaactcg gcgcccgcgc cggcgagcac cgccggcagc
     3121 ccgggggcga cgaactcgaa caccatggtg ccgaacaccg cctcgccctg taacagcctg
     3181 tccttgactc ggttgtcgcg cattcgatcc ctccctcggt cgcgctcgat tatggtcgaa
     3241 acgagcgccg cgacaaccgc cgtcggctgt cgcgcgcggc ccgaatgcgc ttatactgcg
     3301 acggttcgcg agggaggcac cgatgaccgc accccagccg gcgatgatcg cccagccgcg
     3361 cctgttgatg atcggcgccg gcgccgcggc cgaggtcgcg gcggcgatgg ccaagatcgg
     3421 cgtcgcccgg ccgctgatcg tcaccgaccc gtatgtgcgc gacagcggca tgctggcgcg
     3481 cgtgaccgag gtgctggacg gcgccggcgt cccttacgcg ctgttcgccg acaccgtgcc
     3541 cgacccgacc accgaggtgg tcgaggccgg ggtcgcggcg ctcgccgacg gcgacttcga
     3601 ctgcctgatc gcgatcggcg gcggcagccc gatcgacacc gccaaggcga tggcggtgct
     3661 ggcgagccac ggcgggcgga tgcgcgacta caaggtgccg aaccaggtcg atgccaccga
     3721 ctatccgctg atcgccatcc cgaccaccgc cggcaccggc tccgaggtca cccgcttcac
     3781 cgtcatcacc gacaccgaga ccgacgagaa gatgctgtgc gcgggactcg gcctggtgcc
     3841 ggtatgcgcg ctcatcgact acacgctcac cctcagcatg ccgtggcggc tgaccgcgga
     3901 taccggcatc gacaccctca cccatgccat cgaggcctat gtcagccgca agcagaaccc
     3961 gttctcggac gcgatggcgc gctcgtgcat gcgcctcgtc gcgagccaca tccgcaccgc
     4021 ctgcaaggag cccggcaacg ggggcgcgcg cgaggcgatg atgctcgccg cgacccaggg
     4081 cggcatggcg ttctcgaatg cctccgtctg cctggtgcac ggcatgagcc ggccgatcgg
     4141 cgcccatttc cacgtccccc acgggctcag caacgccatg ctgctgccgg cggtgaccgc
     4201 gttctcggcc ggccatgcgc tcgaccgcta cgccgactgt gcgcgcgcca tgggcatcgt
     4261 cgacggggag gtcggcaacc aggccgcggt ggcggtcctg ctcgacgagc tgcgcgcgct
     4321 caatgccgag ctcgaggtgc cgacgccgaa ggggtacggc atcgacgagc gggcctactt
     4381 cgagctgctg cccaagatgg ccgagcaggc gctcgcctcg ggctcgccgg gcaacaaccc
     4441 gcgggtgccc gacgccggcg agatcatgga cctgtaccgc gaggcgtggg cctgaccgag
     4501 gccatggcgg gcggcccggc cgacccccgc tcggaggcgg gggcaggcat cggcgtcgtc
     4561 ggcctcggcg tgatgggcgc gaacctcgcg ctcaacctcg ccgaccacgg cgcggccgtc
     4621 gcggtctaca accgcagcgc cgagcgcgcg gaggcgttcc tggccggcga ggcggcgcgg
     4681 caacgcgcgg tctcgggcca ccgctcgctc gaatccctta tcgccgcgct cgcccgcccg
     4741 cgcaccgtgc tggtgatggt ccaggccggc gcggcggtgg acgcccagat cgacgcgctc
     4801 acgccgctgc tcgatgccgg cgacttggtg atcgacggcg gcaacgccca ctacgccgac
     4861 acggcgcggc gcgccgaggc gctggccgcg gcgggactcg ccttcctcgg catcggcgtc
     4921 tcgggcggcg aggttggcgc gcgctggggc cccgcgatca tggccggcgg cgatgaggcg
     4981 ggatatgcgc gcgcggcgcc ccttctggag gcgatcgcgg ccaaggtcga cggcgacccg
     5041 tgctgcgcct ggctcggtcc cggcggcgcc ggccattacg tcaagatggt ccacaacggc
     5101 atcgagtacg cgctgatgca gctcatcggc gaggcctggc tgatgatgcg cgacgggctc
     5161 gggctcgcgc ccgaggcgat cgcggcggcg ttcgccggct gggccgacgg ccggctcggc
     5221 ggctatctgg tcgagatcac cgccgacatc ctggccgcgc ccgacgccga gaccggcggc
     5281 atgctgctgg acgtgatcga cgaccgcgcc gggcacaagg gcaccggacg ctggtgcgcg
     5341 gaaagcgcgc tcgcgctcgg cgtgccggca gcgaccttgg ccgccggcac tctcgcccgc
     5401 tcgctgtcgg gggcaagcag cgcccggagc ttggcccggc gccggctcga cggtcccgag
     5461 acgcgcgtcg acgacgacat cgaggctgcg ctcgccgacc tcgagcaggc gctgctcgcg
     5521 ggcttcgtga ccgcgttcag cgaaggcttc cagctgctcg acgcggcgtc cgcgcagttc
     5581 gcctggtcgc tcgaccgcgc ccgcatcgcg gcactgtggc gcggcggctg catcatccgc
     5641 gcgcgcctgc tcgacgacat ccgcaccgcg ctcagcgccg cgcccggcac gccgctgctc
     5701 gccgccgagc ccgccttcgc cgcggcgctc gccgaggcgc agccgggctg gcggcggacc
     5761 gtcgcgcgcg ccgtccgcca cggcctgccc gcgccggcaa tggcgagcgc gctcgcggcg
     5821 tatgattcct atcgcaccga gcggctgtgg accgcgctgg tgcaggcgca acgcgactac
     5881 ttcggcgccc acggctaccg gcgcatcgac cgcgtcggcg atgtccacac cgactgggcg
     5941 acgctcgccg gcgatggctg acaccgcggg cctgccggtc gtggtggtgg tgatgggggt
     6001 cgcgggcagc ggcaagacca ccgtcgggca gaggctcgcg gacgcgctcg gcgccgcgtt
     6061 cgaggaaggc gacgactacc atcccgccgc caacgtcgag aagatgcggg gcggggtgcc
     6121 gctcaccgac gccgaccgcg cgccgtggct gcgccgcctc gccgccggca tcgacctgtg
     6181 gcgcgaccaa ggccggcgga ccgtgctcgc ctgctcggcg ctcaaggaga gctatcgcga
     6241 ggtgctgatc ggccgccgcc atgacgtggc gctggtccac ctcattggcc cggaagcttt
     6301 gatccgcatg cgcctggagg caagggtcgg ccattacatg ccggcgcaac tgttggcgag
     6361 ccagctcgcc agcctggagc cgccggggga cgcgatcacg gtcgacgtcg caccggcgcc
     6421 ggagcggatc gtcgaggcga tcctgggccg gctcggcgcg tggcaacgcc aatcattgca
     6481 ggaggagtga gcgatggatc tgcatctcgc cgggcgcacg gcgctggtca ccggcggctc
     6541 gaaggggatc gggcgagcgg tcgcgttcgg gctcgccgcc gagggctgcc atctgcatct
     6601 cgccgcacgc ggtgaggagg ctcttgccaa gacccgcgac gagatcgccc gccgccatca
     6661 ggtgtcggtg accacacatg cgatggacct gtccgacagc gccgcgcagg gcgagctggt
     6721 ggggcgctgc gacggcgtcg acatcctggt caacaacgcc ggcggcatcc cgggcggcac
     6781 gctcgcccag atcgacgagg cgcgctggcg cgaggcgtgg gacctcaagg tgttcggtta
     6841 cgtgaatctg tgccgcgcgt tccatgcccg catgtgcgcg cgcggcgcgg gcgtgatcgt
     6901 caacgtcatc ggcctcgccg gcgagcgctt cgatgccggc tacatcgccg gctccaccgc
     6961 caacgccggg ctgatggcgt tcacccgcgc gctcggctcg accagcctgg agaacggcgt
     7021 gcgcgtggtc ggggtcaatc cgggcctggt cgagaccgac cggatgctgt tcctgatgcg
     7081 cgcccgcgcg caagccgagc tcggcaatcc cgaccgttgg ccggagctga ccaagaatct
     7141 gccgctcggg cgcgcggcgc gcgcggacga ggtggcgagc ctggtcacct tcctcgcctc
     7201 cgaccgcgcc tcctacgtct cgggcgtggt catgaccatc gacggcggcg tcgccgcgcg
     7261 cgcacgctga gacacgcttg cggcgccacc cccaccccgt ccctcccccg cgagcggggg
     7321 agggagtcgc tacactcgaa cctccccccg cgtgcggggg gaggcaggag gggggtgctt
     7381 cggcgcgata gcggtccgga tgcgtcgact gttcagccct gggcggggga catggcgagg
     7441 gtgagcccgc cgtcgaccac cagctcgatg ccggtgatgt agcgcgcctc gtcggaggcc
     7501 aggaacagcg ccgcgcgcgc ggtgtcccag gcatcgccca tgaaccccat cgggcactgg
     7561 gcgttgcgcc tggcgatcat cgcgtcgacg tcgtcat
//
LOCUS       DASWHU010000356        27983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2467140, whole genome shotgun sequence.
ACCESSION   DASWHU010000356 DASWHU010000000
VERSION     DASWHU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 27983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27983
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467140"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1094
                     /locus_tag="VGB88_13165"
     CDS             210..1094
                     /locus_tag="VGB88_13165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9648447.1"
                     /translation="MTNADDRLYDGESLIRAAADADGVFATATEAFPAAVIARLPASV
                     RILGTLSAGTDHIDLAAARARGLVVTSTPDVLSEAVADVAMLLLLAAARRAHEGEALV
                     RSGAWRGWAPTQLLGRELNGHRLGILGMGRIGRALARRAQGFGLKVHYHNRRRLAPQL
                     EHGAHYLADPDSLLAVSEFLSLTCPSNAETRGFLDARRIARLPEGAVVVNVSRGDLVV
                     DDALIAALASGRLAGAGLDVFDGEPDIDPRYRALDNVFLLPHIGSATEQTRTAMGSLV
                     LDGFDAFFAGEEVANRVA"
     gene            1193..1660
                     /locus_tag="VGB88_13170"
     CDS             1193..1660
                     /locus_tag="VGB88_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9648448.1"
                     /translation="MPGRIETRLRDIGIELPKPAAPAANYIPFVTAGSLVFIAGQITF
                     KNGKVAHVGKVGREISVEEAKQAARLCGLNLLAQLRVACAGDLDRVVRCVKLGVFVNC
                     IDGFDRQAEVANGASDLMVEVFGDPGRHARFAVGAYALPRNVAVEADGIFEIA"
     gene            1707..3110
                     /locus_tag="VGB88_13175"
     CDS             1707..3110
                     /locus_tag="VGB88_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX9648449.1"
                     /translation="MSPNEAPRNITLEEMDKHSLLHPYTSIAEHLKSGPVVIAHAKGA
                     RISDIHGKDYIDAMGGLWCVNIGYGRDEMVEAIAAQARALSYYHLFFSMSQEPAIRLA
                     DKVLSLMPKQMSKVFFCNSGSEANDTQVKIAWYYNNLRGKPEKTKIISRRKAYHGVTV
                     AAASMTGLPPVHRSFNLPLPGFLHTSLPHHYWEAPEGMTEREFSAKLAQDLEQMIERE
                     GADTIAAFIAEPVMGAGGVVPPPDGYFEAIQPILSRHDILFIVDEVICGFGRLGRWFG
                     TTYYHLEPDLITIAKGLTSAYVPMSAAVISDKVWQVLKDGSPEVGPFAHGYTYGGHPL
                     AAAAGLKTIEIMERERLVQHAAKVAPYFQRRLREAFADHPLVGEVRGVGLMAGIELVK
                     DKAAKVPFEPPLLVGQRLSRKLLELGMINRGMVNTLCFSPPLVITEADVDEMVDKFAR
                     ALAAMAGELRAEGSWRG"
     gene            3124..4344
                     /locus_tag="VGB88_13180"
     CDS             3124..4344
                     /locus_tag="VGB88_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9648450.1"
                     /translation="MSRVARQRDEGRRGTRFAARLVERIADVGAEAWRRLDDAGNPFV
                     GYEFLSALEDSGCCSAKTGWLPQHVVVEDESGRMVGAMPAYLKSHSFGEYVFDHGWAD
                     AFERAGGRYYPKLQASVPFTPVTGPRLLVDRAADIEVVSGALISAAVQLAARIGVSSL
                     HVTFPTEAEWRRMGEAGLLLRTGEQYHWQNRGYRSFDDFLATLNARKRKMIRKERRRA
                     TEAGMVIRTLCGDDIKPAHWDAFFAFYIDTGSRKWGSPYLNRAFFELLGERLGDRIAL
                     VMCERDGHAIAGALNLVGRDTIYGRNWGCIEYHDVLHFEACYYCAIDYAIEHGLEWVE
                     AGAQGPHKIQRGYLPRRTYSAHWIAEPAFRRAVEEYLARERREVDWEIELVSRDQTPY
                     RKAPGPADSQGGEV"
     gene            4341..4637
                     /locus_tag="VGB88_13185"
     CDS             4341..4637
                     /locus_tag="VGB88_13185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9648451.1"
                     /translation="MSQFAIIVTLKLRPGTGEKFRPLILNNAEAAVRDEPGCHQFQVL
                     CGEDDPETMVFYEVYDSPEALDVHRQQRHYKAFIDGTKDMIAERHVQRCTVIKT"
     gene            4970..5431
                     /locus_tag="VGB88_13190"
     CDS             4970..5431
                     /locus_tag="VGB88_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9648452.1"
                     /translation="MVNDGAEAYRGVIPADCWHEPYMPDAELRREIAAGVAFWGIEED
                     GALVAVMGLQRVGDVDLIRHAYVQGARQRRGLGGELLRSLGARADRPLLVGTWAAAAW
                     AVDFYRKHGFEVVPRAETAALLRRYWSISERQIETSVVLADAAWRARRPAR"
     gene            complement(5428..6432)
                     /locus_tag="VGB88_13195"
     CDS             complement(5428..6432)
                     /locus_tag="VGB88_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9648453.1"
                     /translation="MLDYALKPVLDPDSDPAGARSPAPEAAGDGDLLDAYSRAVARVA
                     EQVGPAVVRVEARGRRGAGSGVVISADGLVLTNSHVVQAAGGARLTTPDGQELEARLI
                     GDDPDTDLALLRADSAAALPAARLADSKTLRVGHLVVAIGNPLGFESTVTAGVVSALG
                     RSLRARSGRLIDDVIQTDAALNPGNSGGPLVSSLGEVVGINTAVILGAQGICFAVASN
                     TASFVVGELIRHGRVRRAYIGVAAQTSAIPRRHARAAGTAAAGAAIISIEPESPAAAA
                     GLAPGDAVVAIDGRAIEGVDDLVRALDGASIARTVTVSVIRRGARRDVSVVPVERSRA
                     "
     gene            6753..6956
                     /locus_tag="VGB88_13200"
     CDS             6753..6956
                     /locus_tag="VGB88_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9648454.1"
                     /translation="MATGKVKWFNPGKGYGFIQPDDGSRDVFVHISAVESAGLSTLTE
                     GQTVSYEIATDRGKTHAVNLRAG"
     gene            complement(7062..8120)
                     /locus_tag="VGB88_13205"
     CDS             complement(7062..8120)
                     /locus_tag="VGB88_13205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9648455.1"
                     /translation="MADATTTEVTPEQQTDLMWEYLKGFHAVHLIRTGVELGLFARIA
                     EAGEAGIDAAGLAARLGLHPPYVAVWCNTAYAWRLVDGDDGPPATFRLAPHVGSILVD
                     RRHARHLAPYFIGATGFYARDLDRYPDFFRTGAVHTFQEHGREFSDGIAATTAGFHPV
                     IARRMLPAIPGLEAKLAQGAKVLDMGCGAAGLMMRIAQTHPAVTCLGIDVDAYGIELA
                     RQRIAEAGLEGRVDVELSDGGEIAHRDEFDVVTMFEVLHEIPPAMRPRVLANCHRALK
                     PGAPLFILDETYPQTLAGLRERAHALAVQTAYNELIWGNVVPTAGEQDALLRGAGFVR
                     IDRAQIATYFTAITAWKG"
     gene            8320..8628
                     /locus_tag="VGB88_13210"
     CDS             8320..8628
                     /locus_tag="VGB88_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9648456.1"
                     /translation="MRRQDIVRILREHEGELRRAGVVSISLFGSAARGEAGLDSDVDL
                     AVRLGAEFSHGGFDYFGKIEDLKARLSRILGQSVDIVEEPVERRSLQDRIDGDRVRAF
                     "
     gene            8654..8965
                     /locus_tag="VGB88_13215"
     CDS             8654..8965
                     /locus_tag="VGB88_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX9648457.1"
                     /translation="MIENADAVARYVAGVDLEGLQADPMRRDAIERCLERISEAAARL
                     GELADELMPGQPWHQVRALGNRLRHEYDRIQTHRLWEIIHDDLPRLRTACDGALRRLR
                     G"
     gene            complement(9089..11416)
                     /gene="clpA"
                     /locus_tag="VGB88_13220"
     CDS             complement(9089..11416)
                     /gene="clpA"
                     /locus_tag="VGB88_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX9648458.1"
                     /translation="MLSKTLEETLHRALAHANARHHEFATLEHLLLSLTEDQDATSVL
                     RACNVDIDRLKRAIEDFLDEELGGLTVEQSVTDAKPTASFQRVIQRAVHHVQMSGREE
                     VTGANVLVAVFSERESHAVYFLHEQDMTRLDAVSYISHGIAKAPGRSETRTPQGSDED
                     DGEKVVKHGTEALDAYCVNLNQKAKEGRIDPLIGRQAEVERTIQVLCRRTKNNPLFVG
                     DPGVGKTAIAEGLARRIVRGEVPEVLINATIFALDMGALLAGTRYRGDFEERVKAVLA
                     ELERYDGAILFVDEVHTVIGAGATSGGAMDASNLLKPALQSGSLRCMGSTTYKEYRNY
                     FEKDRALVRRFQKIDINEPSVEDTIKILEGLKPYYEDYHNVEYTPAALISAVELSHRY
                     IGDRKLPDKAIDVIDEVGAAQALLPEHKRAKIIDQDQIEAIVAKIARIPPKSVSRDDK
                     KMLESLEASLKMVVFGQDAAIGALTNAIKLSRAGLREPEKPIGCYLFSGPTGVGKTEV
                     ARQLAQTMGIELIRFDMSEYMERHTVSRLLGAPPGYVGFDQGGLLTDAIDQHPHAVLL
                     LDEIEKAHPDLFNVLLQVMDHGKLTDHNGKSVDFRNVILVMTTNAGAAELAKAAIGFG
                     REDRAGDDEEAINRMFSPEFRNRLDAVIGFSNLEPETVYRVVDKFVMQLEAQLADRAV
                     TIELTDEARIWLGDKGYDRLYGARPLARVIQEHVKKALADELLFGRLTGGGHVKVQIE
                     AGKVAFDVIPAPKRAKPRKGDGDGDGERKVTEPVN"
     gene            complement(11450..11785)
                     /gene="clpS"
                     /locus_tag="VGB88_13225"
     CDS             complement(11450..11785)
                     /gene="clpS"
                     /locus_tag="VGB88_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX9648459.1"
                     /translation="MGDDGTGGSGGRGRPGTGVVTRTKPKTKKPSMYKVLMLNDDYTP
                     MEFVVYVLERFFHKTREEATRIMLHVHQRGVGICGIYSYEVAETKVTQVMDFARQNQH
                     PLQCTLEKE"
     gene            complement(12030..12560)
                     /locus_tag="VGB88_13230"
     CDS             complement(12030..12560)
                     /locus_tag="VGB88_13230"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9648460.1"
                     /translation="MSQTRTTKTAKAAATGTAEVFAKGQDTVEQLFRAGTEALSGNYD
                     KWIKFNQERATAVLDSFKGWDDVGAQGKETVEAWMAGGRIAAKGLEDITDKWLAFVTA
                     SLEDGVAASRAMFECKDVKQLMELQSKQLRKLIDGWMTEGAEISELTLETATKAMAPI
                     GERVNAAIEKATHPTD"
     gene            13026..14258
                     /locus_tag="VGB88_13235"
     CDS             13026..14258
                     /locus_tag="VGB88_13235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9648461.1"
                     /translation="MAAAALIAALAGGLTVAGPRAGEAGYAALVVDADTGVILHQANA
                     DRTNYPASLTKMMTLYLAFEAIEAKRARLDDRLRVSETAAAQPPSKLGLQAGQTLSLD
                     DAMVALATKSANDVAVVIAEHLAGSEAAFARLMTRRAHELGMTGTSYRNASGLPDDAQ
                     RTNARDISVLARAIYRDFPQYAHYFNNRSFSYHGREYRNTNRLLGVYKGMDGIKTGYI
                     NASGFNLAASVHRGGRHVIAVVLGGKTAPRRDQQMTQLLDRAFTIVDEVRWFDAHVDR
                     PQPKPTFQLAAADGVGGDGSLGNRLGVAAAAVGEALSDYVAIPSASAADTPAREWAIQ
                     VGAFARRDAAERALDTAAMLMPALARGSKRWIEPVVDRYGTLYRARQMGLTWDEATRA
                     CANLSRQRLPCMVAAPGS"
     gene            complement(14286..15038)
                     /locus_tag="VGB88_13240"
     CDS             complement(14286..15038)
                     /locus_tag="VGB88_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9648462.1"
                     /translation="MNDIALAGDSGAALAALSTQLPLARERFAPAGRVGLVIVDEVNG
                     FATVGAGNLAPPEPNAQVARMVAETDRLARAFAARGMPIAAFLDTHQPGKPEPPYPPH
                     CEAGTGEEDLVPELAWLEGEANATLIRKDCINGFVGAIDPASGRNRIIDWIGEHKLDT
                     VLVVGICTDICVMDFVLTLLSARNHRLAPPLKDVVVYEPGCATYHLPRAAAKRLGLPE
                     SASHPQDVTHHMGLYFMHSRGARLAGEVAWAR"
     gene            complement(15109..16497)
                     /locus_tag="VGB88_13245"
     CDS             complement(15109..16497)
                     /locus_tag="VGB88_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9648463.1"
                     /translation="MSRGHALNPTTLRAYDIRGVVGETLDAGDARAVGRAFATMVRAR
                     TGGARICVGYDGRHSSPALESALVEGLCESGAAVTRIGLGPTPLLYFAVKSLGAEAGV
                     MVSGSHNPPAHNGLKMTLASGPFFGDDIQELGRVAAAGAFDDRPGTVAERDVMAAYID
                     VLAGAYRADRGLAVAWDSGNGAAGPAMVRLTARLPGRHVLLCETVDGDFPVHHPDPTV
                     PENLELLIEAVTGQGLDLGLAFDGDGDRLGVVDGQGRILWGDQILCLLAADVLAARPG
                     ATVIADVKASQVLFDQIAALGGVPLMWRTGHSPIKSKMAEVGAPLAGEMSGHIFFADR
                     YYGYDDGLYAGVRLLDVIARSGRSAAELRDALPQMVNTPELRFPCEEARKFAVIDEVA
                     ARLAAAGAEVITVDGVRVNTADGWWLLRASNTQAVLVARAEARDAAGLGRLKAALADQ
                     LRRSGVAPPADL"
     gene            complement(16494..17822)
                     /locus_tag="VGB88_13250"
     CDS             complement(16494..17822)
                     /locus_tag="VGB88_13250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9648464.1"
                     /translation="MRIVMVGTGYVGLVSGACFSEFGVDVTCIDSDRDKIAALAAGRL
                     PIYEPGLEALVARNVAAGRLRFATELAPAMAAADAVFIAVGTPSRRGDGHADLSYVFA
                     AAEQIARAIAVRTVVVTKSTVPVGTGSEVERVIAAARPGLAPGVDFDVASNPEFLREG
                     SAIEDFMHPDRVVVGAASDHARAVLGALYRPLFLRETPIVLTSREAAELTKYAANAFL
                     AAKITFINEIADLCEKVGADVQEVAKGIGLDRRIGAKFLHPGPGFGGSCFPKDALALA
                     RTAREHQVPLHLVETVTAVNEARKAAMAAKVIDACGGSVAAKAIAVLGLTFKPNTDDL
                     RDSPSLVIVPALQKAGATVRAYDPAGMDAARALLPGVAFGSSAYDIAEGAVALVIMTE
                     WNQFRALDLARLRALMARPLMVDLRNVYRPDEVAAAGFDYVGIGRGRAAR"
     gene            complement(17830..18708)
                     /gene="galU"
                     /locus_tag="VGB88_13255"
     CDS             complement(17830..18708)
                     /gene="galU"
                     /locus_tag="VGB88_13255"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX9648465.1"
                     /translation="MAAPVRTAVFPIAGLGSRFLPATKALAKEMLPVIDKPLIQYAVE
                     EAQAAGIERFVFVTGRGKSLIEDHFDHAYELERTLADAGKGALVDVAASARLAPGALF
                     TTRQQEPLGLGHAVWCAREFVGNEPFAVVLPDDLVLHESPCLAQMMAAYERVGGNLVA
                     VMEVPPEHTRRYGILEVGATRGALVEVAGLVEKPEPEVAPSTLSIIGRYILQPAIMDR
                     LARQERGAGGEIQLTDAMAGLIGKAPFHGFRFAGRRFDCGDKLGYIEATLAFALARDD
                     LGTELRAIIRRLAGQE"
     gene            complement(18712..20493)
                     /gene="recJ"
                     /locus_tag="VGB88_13260"
     CDS             complement(18712..20493)
                     /gene="recJ"
                     /locus_tag="VGB88_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX9648466.1"
                     /translation="MAAVGAAPAFLGVERSVAGLRWRSRVDDDGLALALAQALDVPEM
                     LARVLAARKVALDEAEAFLYPTLRALMPDPSGLRDMDRAAARIADAVEGGELIAVFGD
                     YDVDGATASALIRLHLEALGARVRPYIPDRVREGYGPSVAALRILAGEGATLVVTLDC
                     GTTAHGALADAAARGLDVIVVDHHQSGPDLPACHALVNPNRLDDESGLGALAAVGVAF
                     MLAVAVSRELRARGHFAARAEPDLRDRLDLVALGTVCDIVPLTGLNRAFVRHGLKVLA
                     RRANPGLATLADIARIDRAPTAWHAGFVLGPRINAGGRVGKSDLGHRLLTSRSAAEAL
                     PIAQTLDRLNDERKAIESAVLAQALERAEVQAGEGRAMLVVAGAGWHAGVIGLVASRL
                     VEAFARPACVIAVDGGVGKGSARSIAGVDIGAAVIAARQAGLLVDGGGHPMAAGLTAA
                     ADGIDALAAFLDRRVAACADAQRAPSLGLDGALAIAGATVELCGLLDEAGPFGSGNPE
                     PRFALPDCRIAFADVVGAGHVRCRLAGAGGGSLKAIAFRAADAPVGQAVLAARGGALH
                     VAGHLREDRWRGRSEVQLVVDDVARPA"
     gene            complement(20495..21481)
                     /gene="glpX"
                     /locus_tag="VGB88_13265"
     CDS             complement(20495..21481)
                     /gene="glpX"
                     /locus_tag="VGB88_13265"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX9648467.1"
                     /translation="MAETATLDRNLALEVVRVTEAAALSCSRYMGRGDEKVADQAAVD
                     AMRRALNGLAVDGTVVIGEGERDEAPMLYIGEKVGSGGPKVDIALDPLEGTTICATGG
                     ANALSVISMAEQGGFLNAPDVYMDKIAVGGGLPDGVVDLDQAPAANLGNLAKAKGVPV
                     SDLVACILNRPRHEELIAKVREAGARIQLIQDGDVAGVIATSRAGTGIDIYLGIGGAP
                     EGVLAAAALRCIGGQMMGRLVFRNDDERARARNLGIDDLDRKYTLTDLARGEVMFAAT
                     GVTDGSMLRGVRRYAGGASTDSLVMRSKTGTVRVISASHDFTRKAMLGEETR"
     gene            complement(21519..22808)
                     /locus_tag="VGB88_13270"
     CDS             complement(21519..22808)
                     /locus_tag="VGB88_13270"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9648468.1"
                     /translation="MTRPLRVAIAGLGTVGVGTVRLLQTNRELLRQRCGRAIDVVAVS
                     ARDRGKVRGVDLGEARWVDQAEALAEADCDAVVELIGGSGGPALALVRAALARGKPVV
                     TANKAMIAHHGGELAALAEANGVSLGFEAAVAGGIPVIKALRDGLVANRLTRVYGILN
                     GTCNYVLTTMRETGRDFAEVLAEAQALGYAEADPGVDVDGIDAAHKLAILASLAFGTR
                     IDFDSVHVEGIRHVSPVDIAFADDLGYRIKLLGIARQLEAGLEQRVHPCMVPSAAPIA
                     AVEGVLNAVVGEGNFIGSTVFEGPGAGAGPTASAVVADLVDIARGQRVPTFGVPAAEL
                     VELAAVPIERRTGPYYVRLMVVDRPGVIADIAACLRDHDVSMEAMIQRARKPDEAVPV
                     VMTTHDVIEASIGAALRQIAALESVCEPPRMIRIESL"
     gene            complement(22805..24019)
                     /locus_tag="VGB88_13275"
     CDS             complement(22805..24019)
                     /locus_tag="VGB88_13275"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX9648469.1"
                     /translation="MTDEFERIRRLPPYVFAEVNEMKRRARAAGRDIIDFGMGNPDSP
                     TPRHIVEKLVETVRDPRAHRYSVSRGIPGLRRAMAAYYRRRFGVELDPEREVIVTLGS
                     KEGLANLASAMTSPGDIILATNPSYPIHPYGFVIAGAAVHHVPSGPGVDVLAELERAV
                     AACPRTPKAVVVNYPNNPTAEVVDLDFYRRLVAIARKHGIYVLSDLAYAEIYFECAPP
                     PSILEVPDAREVAVEFTSLSKTYSMPGWRIGFAAGNARLIGALARIKSYLDYGAFTPI
                     QVAATAALNGPQECIDEIRARYRERRDVLIDSFAAAGWPLPKPVATMFVWAPIPAAWQ
                     PVGSLEFSKRLLEHAEVAVSPGIGFGEYGEGHVRIALVENRQRIRQAARNLKAFLATV
                     EVPEAPRRAAAS"
     gene            complement(24084..25295)
                     /locus_tag="VGB88_13280"
     CDS             complement(24084..25295)
                     /locus_tag="VGB88_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9648470.1"
                     /translation="MDLLSPWRSVHQVASAVAVPVRVWALGGLFGLALAGCSAMPDAL
                     NPVEWVSSADSYLSGEDEAADPELAARAEQEQAEPVPGADEPFPSLGAVPGQAPKVTA
                     YDERQQIAQGLVADRAAARYVDNPAAEAVMREPALAWREPTAPAVERAPEPEIAAGAP
                     APAVTVTEAAPMPAPTPMAAAEPEPETLPEPMPVPAAGPVASAPMPMPATAVETEAMP
                     WLEAAPVPMAASAPRRETMAPPAPVATATPALEAPASVRVAAAAPVLASAAAPASRPL
                     PVEASGPAMGSVHAGVIYFAYGSTSLSAADTQVLGQIADLYRSRGGRVRVVGHSSGRV
                     DGTDEVKAKLANFAVAFDRARMVAEELARQGVPADQIFPSSMSSDAPAYSETTKLGEA
                     ANRRADIYIDF"
     gene            25534..27333
                     /gene="phaC"
                     /locus_tag="VGB88_13285"
     CDS             25534..27333
                     /gene="phaC"
                     /locus_tag="VGB88_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX9648471.1"
                     /translation="MTETDREPDTAAGTLQAAETWQRIAERSQRIVQRFLTRHAGEET
                     PFGDPANLVGAFAEMTGRLFADPARLMQAQMDLWQSHMRLWTNTAERLLGSRRPEPVA
                     APQPEDRRFRDQAWEENAVFDYIKQSYLVTARWLQSTVRGVEGLDDKTARKVDFYTRQ
                     FVDAMAPTNFVLTNPEVLRETVDSKGENLVRGLENLLHDLERGDGELRISMTDMAAFE
                     VGKNVAVTPGEVMFRNDMIELIQYAPTTATVHKRPLLVVPPWINKFYILDLRPRNSFV
                     KWATDQGYTVFVISWVNPDRKLAEKTFDDYMLEGPIAALDAIEQATGEREACAASLCL
                     GGTLLACTLAYLAAIGDTRIKSATLMASLVDFSEPGELGVFIDDEQLAALETRMRQKG
                     GYLDGGAMSTTFNMLRANDLIWSFVINNYLLGKEPLPFDLLYWNADSTRMPARMHSFY
                     LRNMYQNNRLIQPGGIELAGEKIDLGKVKTPVYVLATREDHIAPWRSAYAAVHLFAGP
                     VRFVLGGSGHIAGITNAPAVAKYPYWTNRRKPKSPDAWLDSASAHQGSWWTDWDKWQA
                     RHAGPLVPARTPGTGRLASLGPAPGAYVLQKEK"
     gene            27340..>27983
                     /locus_tag="VGB88_13290"
     CDS             27340..>27983
                     /locus_tag="VGB88_13290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9648472.1"
                     /translation="MALPNTPRKTLVLTGASRGIGHATVKRFSSAGWRVITCSRMAFP
                     EGCPWEAGPEDHLQIDLGDSDSLDERIAQLKRMLDGSPLHALVNNAAISPKGPDGARL
                     DSLSTPMADWHQVFGVNFFAPIRLARGLIEELKKGPGTIVNVTSIAGGRVHPFAGTAY
                     ATSKAALASLTREMAYDFGRLGIRVNAIAPGEIRTAILSPDTEAQMLPDIPLGRL"
BASE COUNT         4077 a         9952 c         9926 g         4028 t
ORIGIN      
        1 tcgcaagcac ggcccggccg atgtcgaagg tcattcggtc gccttccgcg tggtttgggc
       61 ttgcgcgcgg cacggcgggc gtgccatcga ccatcgcgtc ccccagcccg cgatgaatat
      121 agagagcgat gtcgaacaaa aaaaggattc tggcgacgcg ccgcttgccc gacgcggtcg
      181 aggcgcgtct tgcgcgcgac tacgcggcaa tgacgaacgc cgatgaccgc ctctacgacg
      241 gcgaatctct gattcgcgcc gcggccgacg ccgacggcgt gttcgccacc gcgaccgagg
      301 cgttccccgc ggcagtgatc gcgcggctgc ccgcgagcgt gcgaatcctc ggcacgctct
      361 cggccggcac cgaccacatc gacctcgccg ccgcccgggc acgcggcctg gtggtgacgt
      421 ctaccccgga cgtgctcagc gaagccgtcg ccgacgtcgc catgctgctg ctgctggcgg
      481 ccgcgcgccg cgcccatgag ggtgaggcgc tggtgcgcag cggcgcctgg cgcggctggg
      541 cgccgaccca gctgttgggg cgcgagctca acggccaccg gctcggcatc ctcggcatgg
      601 gccggatcgg gcgcgcgctg gcgcggcgcg cgcagggctt cggcctcaag gtccactacc
      661 acaaccgccg ccgccttgct ccgcaactcg agcatggcgc gcactatctc gccgaccccg
      721 actcgctcct cgcggtgagc gagttcctgt ctctgacctg cccgtcgaat gccgaaacgc
      781 gcggcttcct cgacgcaagg cgcatcgcgc gtctgcccga gggcgcggtg gtggtcaacg
      841 tctcgcgcgg cgacctggtg gtcgacgacg ccctgatcgc cgcgctggca agcggccggc
      901 tcgccggcgc cggcctcgac gtgttcgacg gcgagcccga catcgacccg cgctaccgcg
      961 ccctcgacaa cgtcttcctg ctgccgcaca taggcagcgc caccgagcag acgcgcaccg
     1021 cgatgggcag cctggtgctc gacggcttcg acgccttctt cgccggcgag gaggtggcga
     1081 accgggttgc gtgacgcgcc cgaccggcgc cgatttgcct ctggcgcgct atcgccatgg
     1141 cgcctatcct cgccgccgac cagatcatcc ccggcagctt gaggagacgc ggatgcccgg
     1201 ccggatcgag accaggttgc gcgacatcgg catcgagctg cccaagccgg cggcgccggc
     1261 ggccaactat attccgttcg tgaccgccgg ctcgctggtg ttcatcgccg gccagatcac
     1321 gttcaagaac ggcaaggtcg cccatgtcgg caaggtcggg cgcgagatct cggtcgagga
     1381 agccaagcag gcggcgaggc tgtgcggcct caacctgctg gcccagctgc gagtggcgtg
     1441 cgccggcgac ctcgaccggg tggtgcgctg cgtcaagctc ggggtgttcg tcaactgcat
     1501 cgacggcttc gaccgccagg ccgaggtcgc caacggcgcc tccgacctga tggtcgaggt
     1561 gttcggcgac cccggccgcc acgcccgctt cgcggtcggc gcctacgcgc tgccgcgcaa
     1621 cgtcgcggtc gaggccgacg gcatattcga gatcgcatga gccgcgtgct cgcggccaaa
     1681 acgggggccc gccaaggagt aacgacatga gcccgaacga agctccgcgc aacatcacgc
     1741 tcgaggagat ggacaagcac agcctgctcc atccctacac ctcgatcgcg gagcatctga
     1801 agagcggccc ggtggtgatt gcgcacgcca agggcgccag gatcagcgac atccacggca
     1861 aggactacat cgacgcgatg ggcggtctgt ggtgcgtcaa catcggctac ggccgcgacg
     1921 agatggtcga ggcgatcgcc gcccaggcgc gcgcgctgtc ctactaccac ctgttcttct
     1981 cgatgtcgca ggagccggcg atccgcctcg ccgacaaggt gctgagcctg atgccaaagc
     2041 agatgagcaa ggtgttcttc tgcaactcgg gctcggaggc caacgacacc caggtcaaga
     2101 ttgcctggta ctacaacaat ctgcgtggca agcccgagaa gaccaagatc atctcgcggc
     2161 gcaaggccta tcacggcgtc accgtcgccg cggccagcat gaccgggctg ccgccggtgc
     2221 acaggtcgtt caacctgccg ctgcctggct tcctgcacac ctcgctgccg caccactact
     2281 gggaggcgcc tgagggcatg accgagcgcg agttctcggc caagctcgcc caggacctcg
     2341 agcagatgat cgagcgcgag ggcgccgaca cgatcgccgc gttcatcgcc gagccggtga
     2401 tgggcgcggg cggcgtggtg ccgccgccgg acggttattt cgaggcgatc cagccgatcc
     2461 tctcccgcca cgacatcctg ttcatcgtcg acgaggtgat ctgcggcttc ggccggctcg
     2521 ggcgctggtt cggcaccacc tactaccacc tcgagccaga cctgatcacc atcgccaagg
     2581 gcctgaccag cgcctatgtg ccgatgtcgg cggcggtgat ctcggacaag gtgtggcagg
     2641 tgctgaagga cggctcgccc gaggtcggcc cgttcgccca tggctacacc tatggcggcc
     2701 acccgctcgc cgccgccgcc gggctgaaga cgatcgagat catggagcgc gagcgcctgg
     2761 tccagcacgc ggccaaggtg gcgccctatt tccagcgccg gctgcgcgag gcgttcgccg
     2821 accatccgct ggtcggcgag gtgcgcggcg tcggcctgat ggccggcatc gagctggtca
     2881 aggacaaggc ggccaaggtg ccgttcgagc cgccgttgct ggtcggccag cgcctgagcc
     2941 gcaagctgct cgagctaggc atgatcaacc gcggcatggt caacacgctg tgcttctcgc
     3001 cgccgctggt aatcaccgag gcggacgtcg acgagatggt cgacaagttc gcccgcgcgc
     3061 ttgccgccat ggccggcgag ctgcgcgccg aaggcagctg gcgcggctga tcgcgggcgg
     3121 ggaatgagcc gggtcgcgcg ccagcgggac gaaggccggc gcggcacgcg cttcgccgcc
     3181 cggctggtcg agcgcattgc cgacgtcggt gccgaggcgt ggcgccgtct cgacgacgcc
     3241 ggcaatccgt tcgtcggcta tgagttcctc agcgccctcg aggacagcgg ctgctgctcc
     3301 gccaagaccg gctggctgcc gcaacacgtc gtggtcgagg acgagtccgg ccgcatggtc
     3361 ggcgcgatgc cggcctacct caagagccac tcgttcggcg agtacgtgtt cgaccacggc
     3421 tgggcggacg cgttcgagcg cgcgggcggg cgctactatc ccaagctcca ggcctcggtg
     3481 ccgttcacgc cggtgaccgg gccaaggctg ctggtcgacc gcgcggcgga tattgaggtt
     3541 gtaagcggcg cgctgatctc cgccgcggtc cagctcgcgg cgcggatcgg ggtctcatcg
     3601 ctccacgtca ccttcccgac cgaggcggaa tggcgccgga tgggcgaggc cggactcttg
     3661 ctgcgcaccg gcgagcagta ccactggcag aaccggggct atcggagctt cgacgacttc
     3721 ctcgccacct tgaacgcgcg caagcgcaag atgatccgca aggagcgccg gcgcgcgacc
     3781 gaggccggca tggtcatccg cacgctgtgc ggcgacgaca tcaaacccgc ccactgggac
     3841 gcgttctttg ccttctatat cgacaccggc agccgtaaat ggggctcgcc ctatctcaac
     3901 cgcgcgttct tcgagctgtt gggcgagcgg ctcggcgacc gcatcgcgct ggtgatgtgc
     3961 gaacgcgacg ggcatgcgat cgcgggcgcg ctcaacctgg tcgggcgcga cacgatctat
     4021 ggccgcaatt ggggctgcat cgagtaccac gacgtcctgc atttcgaggc gtgctactac
     4081 tgcgccatcg attacgccat cgagcacggc ctggagtggg tcgaggccgg cgcccagggc
     4141 ccgcacaaga tccagcgcgg ctacctgccc cggcgcacct acagcgcgca ctggatcgcc
     4201 gagcccgcgt tccgccgcgc ggtcgaggag tacctggcgc gcgagcgccg cgaggtggac
     4261 tgggagatcg agctcgtgtc ccgcgaccaa acgccctatc gcaaggcgcc ggggccggcc
     4321 gacagccagg gaggagaagt atgagccagt tcgcgatcat cgtcaccctc aagctcaggc
     4381 ccggcacagg cgagaagttc cgcccgctga tcctcaacaa cgccgaggcg gcggtgcgcg
     4441 acgagcccgg ctgccaccag ttccaggtgc tgtgcggcga ggacgacccc gagaccatgg
     4501 tcttctacga ggtctacgac agccccgagg cgctcgacgt ccaccgccag cagcgtcact
     4561 acaaggcgtt catcgacggc accaaggaca tgatcgccga gcgccacgtc cagcgctgca
     4621 cggtgatcaa gacctgacgc ttttaagtca gtgaaaaggg atcaaccaca gaggacacag
     4681 agaatacaga ggggggacag tcattgcgag cggagcggag caatctagaa acgtggcgat
     4741 gtgccgggcc aacaactgga tcgcggcggc gctacgcgcc tccaccctcc gcagcggcag
     4801 cgccgctgct gcggaggacg ggtcgcgatg acgtgtacgg tgagccctct atgttccccg
     4861 tgtcctctgt ggttcaatct tctttcctat gaacacggaa cctaatggca gcgcggttgc
     4921 tcacgccgtc cgacgctgcc gcacggacga cttcgacgac atcctcgccg tggtcaacga
     4981 cggcgcggaa gcctatcgcg gcgtgattcc cgccgactgc tggcacgagc cgtacatgcc
     5041 ggacgcggag ctccgccgcg agatcgccgc cggcgtcgcg ttctggggca tcgaggagga
     5101 tggcgccctg gtcgccgtga tgggcctgca gcgggtgggg gacgtggact tgatccgcca
     5161 cgcctatgtg cagggcgccc ggcagcggcg cggcctcggc ggcgagctgc tgcggtcgct
     5221 cggcgcgcgc gcggacaggc cgctcttggt cggcacctgg gcggcggccg cgtgggcggt
     5281 cgacttctac cgcaagcacg gcttcgaggt ggtgccgcga gcggagacgg cggcgctgtt
     5341 gcgccgctac tggtcgatat ccgaacgcca gatcgagacc tctgtggtgc tcgccgacgc
     5401 cgcctggcgt gcgcgccgcc cggcccgcta agcgcgcgag cgctcgaccg gcacgacgga
     5461 tacgtcgcgg cgcgcgccgc ggcggataac agacacggtc accgtgcggg cgatgctggc
     5521 gccgtcgagc gcccgcacca ggtcgtcgac gccttcgatc gcgcgcccgt cgatcgccac
     5581 cacagcgtcg cccggggcga ggccggcggc ggccgccggg ctttcgggct cgatgctgat
     5641 gatcgccgcg ccggcggcag cggtgccggc ggcgcgcgcg tggcggcgcg ggatcgcgct
     5701 ggtctgcgcg gcgacgccga tataggcgcg gcgcacccgg ccgtggcgaa tcagctcgcc
     5761 gaccacgaag ctcgcggtgt tgctggcgac cgcaaagcag atgccctggg cgccgaggat
     5821 gaccgcggtg ttgatgccga ccacctcgcc cagcgaggac accagcggtc cgcccgagtt
     5881 gcccgggttg agcgccgcgt cggtctggat gacgtcgtcg atcagccggc cgctgcgcgc
     5941 ccgcaggctg cgcccgagcg ccgagaccac gccggcggtg acggtcgact cgaaaccgag
     6001 cggattgccg atcgcgacca ccagatggcc gaccctgagc gtctttgaat cggcgagccg
     6061 cgccgccggc agggccgcgg cgctgtccgc gcgcagcagc gcgagatcgg tgtcgggatc
     6121 atcgccgatc agccgcgcct ccagctcctg cccgtcgggc gtggtcagcc gcgccccgcc
     6181 ggcagcctgc acgacatggc tgttggtcag caccagcccg tcggcggaga tgacgacgcc
     6241 ggagccggcg ccgcgccgcc cgcgggcctc gacgcggacc acggccggac cgacctgctc
     6301 cgcgacccgc gccaccgcgc gcgagtaggc gtcgagcaga tcgccgtcgc cggccgcctc
     6361 gggtgccggt gagcgcgcgc ccgccgggtc cgagtcgggg tccaggacgg gtttgagcgc
     6421 gtaatcgagc atggcgggcc gtgaccaatc ggggcacaac aggtttgtcg tcggccatgc
     6481 cgtttcaagg cggcccgcgg ccacccggtg cgcggcctcg gcggcggccg atccgccgcg
     6541 cgccgcggcg tcgccaggca cggctcgccc cttgccatcg ccatcgagcc gggttgcaat
     6601 cgggacggcg ccggggcatg gtaatgtcac cagtctttgc ttccggcgct cttgccgttc
     6661 ggcctcgggg cgatcgtcca ggacatctcg gcaagcgacg attgtgtcgg cgtacgcgac
     6721 ggatgcgtgc atgccaccca gaggaggcca atttggctac aggcaaggtg aagtggttca
     6781 acccgggcaa ggggtacggg ttcattcagc cggacgacgg ctcgcgcgac gtgttcgtgc
     6841 acatcagcgc cgtcgagagc gcgggtctga gcaccctgac cgagggacag acggtcagct
     6901 acgagatcgc caccgatcgc ggcaagaccc acgccgtcaa cctccgggct ggataacccc
     6961 agcccgcgat cgatgccgcc gacggcggcc cgccacgggc cgccgcggtt gcgcttcacc
     7021 atcgactcgg cacgccccgc cgatcgtcgc ggccggccgg ctcagccctt ccaggcggtg
     7081 atcgcggtga agtaggtcgc gatctgggcg cggtcgatgc gcacgaagcc ggcgccccgc
     7141 aacagcgcgt cctgctcgcc ggcggtcggc accacgttgc cccagatcag ctcgttgtag
     7201 gcggtctgca ccgccagcgc gtgcgcgcgc tcgcgcaagc ccgcaagcgt ctgcggatag
     7261 gtctcgtcga ggatgaacag cggcgcgccg ggcttgagcg cccggtggca gttggccagc
     7321 acgcgcgggc gcatcgcggg cggaatctcg tgcagcacct cgaacatggt gacgacgtcg
     7381 aactcgtccc ggtgcgcgat ctcgccgccg tccgagagct cgacgtcaac ccggccctcg
     7441 aggcccgcct cggcgatgcg ctggcgcgcg agctcgatgc cgtaggcatc gacgtcgatg
     7501 ccgaggcagg tgaccgcggg atgggtctgg gcgatgcgca tcatcagccc ggcagcgccg
     7561 cagcccatgt cgagcacctt ggcgccttgg gcgagcttcg cctcgaggcc cgggatcgcc
     7621 ggcagcatgc gccgcgcgat caccgggtgg aagccggcgg tggtggcggc aatgccgtct
     7681 gagaactcgc ggccgtgctc ctggaaggta tgcacggcgc cggtgcggaa gaagtcggga
     7741 taacggtcga ggtcgcgcgc gtagaacccg gtcgcgccga tgaagtaggg tgccaagtgg
     7801 cgcgcatgcc ggcggtcgac caggatcgag ccgacatgcg gggcaaggcg gaaggtcgcc
     7861 ggcggcccgt cgtcgccgtc gaccagccgc caggcgtaag ccgtgttgca ccataccgcg
     7921 acatagggcg gatgcaggcc gagccgcgcg gcgagcccgg cggcgtcgat gccggcctcg
     7981 ccggcctcgg cgatgcgcgc gaacaggccg agctcgacgc cggtgcgaat caggtgcacg
     8041 gcgtggaagc ccttcaggta ctcccacatc aggtcggtct gctgctcggg cgtaacctcc
     8101 gtcgtcgtcg cgtccgccat gacctcctcc cccgcctgtt gcccggcggc actttggccg
     8161 ataaccgagg ccgagacaaa cctcgcggga gcagcccgac gaacaccgtg gaggcggtgt
     8221 ggacggtcgc cgcgcagcgg gcacggccgc acccgatcgg cgaccgcccg ccggcgatcc
     8281 ggcgatgacc gggggcagcg cgcggcccat aatacggtca tgaggcgcca ggacatcgtc
     8341 aggatactgc gcgagcatga gggcgagctg cggcgcgccg gggtggtcag catctcgctg
     8401 ttcggctcgg ccgcgcgggg cgaggcaggc ctcgactcgg acgtcgacct ggcagtccgg
     8461 ctgggcgcag aattctccca cggcggcttc gactatttcg gcaagatcga ggatttgaag
     8521 gcgcggctgt cgcggattct cgggcaatcc gtcgacatcg tcgaggagcc ggtcgagcgg
     8581 cggagccttc aggaccgcat cgacggagat cgcgtccgtg ccttctgaaa aggccgctcg
     8641 caggctgacc gatatgatcg agaacgccga tgcggtcgcc cgctacgtgg cgggagtcga
     8701 tctcgaaggt ttgcaggccg atccgatgcg ccgcgacgca atcgagcgat gcctggaacg
     8761 gatcagcgaa gcggcggcga ggcttgggga gctggcggac gagctgatgc ccggtcagcc
     8821 gtggcatcag gtcagggcgc tcggcaaccg gctgcgccac gagtacgacc ggatccagac
     8881 gcatcgtttg tgggaaatca tccacgatga cctgccccgt ctgcgaaccg cctgcgatgg
     8941 ggcgctacgc cgcctccggg gatagcggca atggcggtgc tcgatcggat gcttgcgggc
     9001 gcgccggcga tgggacggta aggcggcgca gcgaaaaggc cgccccgaag ggcggcctga
     9061 tgtcgcttga acgctggacc gggcgcgctc agttgaccgg ctcggtcacc ttgcgctcgc
     9121 cgtcgccgtc gccgtcgccc ttgcgcggct tcgcccgctt gggcgcgggg attacgtcga
     9181 atgcgacctt gccggcctcg atctggacct tgacgtggcc gccgccggtg agcctgccga
     9241 acagcagctc gtcggcgagc gccttcttga cgtgctcctg gatcacccgc gccagcggcc
     9301 gtgcgccata gagccggtcg tagcccttgt cgcccagcca gatgcgcgcc tcgtcggtga
     9361 gctcgatggt gaccgcgcga tcggcgagct gggcctcgag ctgcatcacg aacttgtcga
     9421 ccacgcggta gacggtctcg ggctcgaggt tgctgaaccc gatcaccgca tccaggcggt
     9481 tgcggaactc gggcgagaac atgcggttga tcgcctcctc gtcgtctccc gcccggtcct
     9541 cgcggccgaa gccgattgcc gccttggcca gctccgcggc gccggcattg gtggtcatga
     9601 ccaggatcac gttgcggaaa tcgaccgact tgccgttgtg gtcggtgagc ttgccgtggt
     9661 ccatcacctg cagcagcacg ttgaacaggt cgggatgcgc cttctcgatc tcgtcgagca
     9721 gcaggaccgc gtgcggatgc tggtcgatgg cgtcggtcag cagcccgccc tggtcgaagc
     9781 cgacatagcc cggcggcgcg ccgagcaggc gcgagaccgt gtgccgctcc atgtactccg
     9841 acatgtcgaa gcggatcagc tcgatgccca tggtctgggc gagctggcgc gcgacctcgg
     9901 tcttgccgac gccggtcggg cctgagaaca ggtagcagcc gatcggcttc tcgggctcgc
     9961 gcaggccggc gcgcgacagc ttgatggcgt tggtcagcgc gccgatcgcg gcatcctggc
    10021 cgaacaccac catcttgagg ctcgcctcga gcgattccag catcttcttg tcgtcgcggc
    10081 tgacgctctt gggcgggatg cgcgcgatct tggcgacgat cgcctcgatc tggtcctggt
    10141 cgatgatctt ggcgcgcttg tgctcgggca gcagcgcctg ggcggcgccg acctcgtcga
    10201 tcacgtcgat cgccttgtcc ggcagcttgc ggtcgccgat gtagcggtgc gacagctcga
    10261 ccgcgctgat cagcgccgcc ggcgtgtact cgacgttgtg gtagtcctcg tagtagggct
    10321 tcagcccctc gaggatcttg atcgtgtcct cgaccgacgg ctcgttgatg tcgatcttct
    10381 ggaaccggcg caccagcgcc cggtccttct cgaagtagtt gcggtactcc ttgtaggtgg
    10441 tcgagcccat gcagcgcagg ctgccgctct gcagcgccgg cttgagcagg ttggaggcgt
    10501 ccatggcgcc gcccgaggtg gcgccggcac cgatcacagt gtggacctcg tcgacgaaca
    10561 ggatggcgcc gtcatagcgc tcgagctcgg cgagcaccgc cttgacccgc tcctcgaagt
    10621 cgccgcgata gcgggtgccg gccaagagcg cgcccatgtc gagcgcgaag atggtggcgt
    10681 tgatcagcac ctcgggcacc tcgccgcgca cgatccggcg cgccaggccc tcggcgatcg
    10741 cggtcttgcc gaccccgggg tcgccgacga acagcgggtt gttcttggtc cgccggcaca
    10801 gcacctggat ggtccgctcg acctccgcct ggcgcccgat cagcgggtcg atgcgtccct
    10861 ccttggcctt ctggttgagg ttgacgcaat aggcgtcgag cgcctcggtg ccgtgcttga
    10921 ccaccttctc gccgtcgtcc tcgtccgagc cctggggggt gcgggtttcg ctccgcccag
    10981 gcgccttggc gatgccgtgg ctgatgtagc tgaccgcgtc gagccgggtc atgtcctgct
    11041 cgtgcaggaa gtagacggcg tgcgattcgc gctccgagaa caccgcgacc aggacgttgg
    11101 cgccggtgac ctcctcgcgc cccgacatct ggacgtggtg gaccgcgcgc tggatgacgc
    11161 gctggaagct cgcggtcggc ttggcgtcgg tcaccgactg ctcgacggtg agcccgccga
    11221 gctcctcgtc gaggaagtcc tcgatcgccc gcttgagccg gtcgatgtcg acattgcacg
    11281 cgcgcagcac cgaggtcgcg tcctggtcct cggtcagaga cagcagcagg tgctcgagcg
    11341 tggcgaactc gtggtggcgc gcgttcgcgt gcgcgagcgc ccggtgcagg gtctcctcca
    11401 gcgtcttaga cagcatggca ctcggatcct cggacctgcc gcatggggat cactccttct
    11461 ccagcgtgca ttggagggga tgctggttct gtcgcgcgaa gtccatcacc tgagtcacct
    11521 tagtctcggc gacctcgtag gaatagatgc cgcagatgcc gaccccgcgc tgatggacgt
    11581 gtagcatgat ccgggtcgct tcctcgcgcg tcttgtggaa aaaccgctcc agaacgtaga
    11641 ccacgaactc catcggcgtg tagtcgtcgt tgagcatcag caccttgtac atcgagggct
    11701 tcttggtctt gggcttggtg cgcgtgacca cgccggtgcc cggccggccg cgcccgccgg
    11761 agcccccggt cccgtcgtcg cccatgcgcg gggcacgcgc gctgatcaga gccgttcgac
    11821 ggagcatcgc ctgcgatcga ccctcactcc tggccccgcc gccggcgccg gtcgcgcgcg
    11881 gcggcctcgg ctggtccctc gctgctataa tatcgaacgc ggccagataa attaaagact
    11941 tgaccccggc cgggcgcgcg ctcccgaaaa aaaagacggc ccgacgccgg gcgccgggcc
    12001 atccgcccag ggagacgtgc ggggaaggct tagtcggtgg gatgggtcgc cttctcgatc
    12061 gcggcgttga cccgctcgcc gatcggcgcc atcgccttgg tcgcggtctc gagggtcagc
    12121 tccgaaatct cggcgccctc ggtcatccag ccgtcgatca gcttgcgcag ctgcttggac
    12181 tggagctcca tcagctgctt cacgtccttg cactcgaaca tcgcccgcga ggcggcgaca
    12241 ccgtcctcga gcgacgcggt gacgaaggcc agccacttgt ccgtgatgtc ctcgagaccc
    12301 ttggccgcga tccggccgcc ggccatccag gcctcgacgg tctccttgcc ctgggcgccg
    12361 acgtcgtccc agcccttgaa gctgtcgagg acggcggtcg cgcgctcctg gttgaacttg
    12421 atccacttgt catagttgcc ggacagcgct tcggtgcccg cccggaacag ctgctcgacg
    12481 gtgtcctggc ccttggcgaa cacctcggcg gtgccggtgg cggcggcctt ggcggtcttg
    12541 gtggtcctgg tctggctcat ggtcggtgcc ttcctcgtgg tcatgccctt gggaacccgt
    12601 tcgccgctcg tgcgcccaca gggtggaacg gcgcccctgc gggtttgcga ccgccgccgc
    12661 cggggtggtc cctccaggcg gtttattgtg cggtgcaaca ttgaccgcca atttaggctt
    12721 ggttacgccc gtgtcaaggg gatttgtgcg gcgcaataca attaggtgcg gcgcaaccaa
    12781 tgcgcctccg gcaggcgtcc gcgcgccggt ccggctgcat tctcccgcgc gccaggccgg
    12841 ttgcgcgggc cccggtcggg cttgattgcc gtcaccaaat ttgaggctcg cctttgaaat
    12901 tcctacgaat caatatagaa attatactca ggcaaactcg acggggggct gcgcgactcg
    12961 tgcggagggg atggggattc cgagcggacg gcgacccgag gcattcctcg ggccggcggt
    13021 cgcgggtggc ggcggcggcg ctgatcgcgg ccctggccgg cgggctgacg gtcgccggtc
    13081 cgcgtgccgg cgaggccggc tacgccgcgc tggtggtcga cgccgacacc ggcgtcatcc
    13141 ttcatcaggc caatgccgac cggaccaatt atccggcgtc gctgaccaag atgatgaccc
    13201 tgtacctggc cttcgaggcg atcgaggcca agcgagcgcg gctcgacgat cggctgcggg
    13261 tctccgagac cgccgcggcc cagccgccct cgaagctcgg cctccaggcc ggccagacgc
    13321 tcagcctcga cgacgccatg gtggcgctgg cgaccaagtc ggcgaatgac gtcgcggtgg
    13381 tcatcgccga gcacctggcc ggctcggagg ccgcgttcgc gcggctgatg acccggcgcg
    13441 cgcacgagct cggcatgacc ggcaccagct atcggaatgc ctccgggctg cccgacgacg
    13501 cccagcgcac caacgcccgc gacatcagcg tgctggcgcg cgccatctat cgcgattttc
    13561 cccagtacgc gcactacttc aacaaccgca gcttcagcta ccacggccgc gagtaccgca
    13621 acaccaaccg cctgctcggc gtctacaagg gcatggacgg gatcaagacc ggctacatca
    13681 acgcctcggg cttcaacctg gccgcgtcgg tccatcgcgg cggccgtcac gtcatcgccg
    13741 tcgtcctggg cggcaagacg gcgccgcgcc gcgaccagca gatgacgcag ttgctcgacc
    13801 gggcgttcac catcgtcgac gaggtccgct ggttcgacgc ccatgtcgat cggccacagc
    13861 ccaagccgac gttccagctc gcggcggccg acggcgtcgg cggcgacggc tcgctcggca
    13921 atcgcctggg cgtcgccgcg gcggcggtcg gcgaggcgct gtccgattac gtggcgatcc
    13981 cgtcggcgag cgcggccgac acgccggcgc gcgagtgggc gatccaggtc ggcgccttcg
    14041 cgcggcgcga tgccgccgag cgcgcgctcg ataccgcggc gatgctgatg ccggccctgg
    14101 cgcgcgggag caagcgctgg atcgagccgg tggtcgaccg ctacggcacc ctctatcggg
    14161 cgcggcagat gggcctgacc tgggacgagg cgacgcgcgc ctgcgccaac ctcagccgcc
    14221 agcggctgcc gtgcatggtc gccgcgccgg gctcgtaggc tgcgaccccc gcaccggccc
    14281 ggggctcagc gcgcccaggc cacctcgccc gccaggcgcg cgccgcgcga atgcatgaaa
    14341 tagagaccca tgtggtgggt cacgtcctgc gggtggctgg cgctctcggg tagccccagc
    14401 ctcttcgccg ccgcgcgcgg caggtggtag gtcgcgcagc ccggctcgta gaccaccacg
    14461 tccttgagcg gcggcgccag gcggtgattg cgcgccgaca gcagggtcag cacgaaatcc
    14521 atcacgcaga tgtcggtgca gatgccgacc acgagcacgg tgtcgagctt gtgctccccg
    14581 atccagtcga tgatccggtt gcgcccgctc gccgggtcga tcgcgccgac gaagccgttg
    14641 atgcagtcct tgcggatcag ggtcgcgttg gcctcgccct cgagccaggc gagctcgggc
    14701 accaggtcct cctcgccggt gcctgcctcg caatgcggcg gatagggcgg ctcgggcttg
    14761 cccggctggt gggtgtcgag gaaggccgcg atcggcatgc cgcgcgcggc gaaggcgcgc
    14821 gccaggcggt cggtctcggc gaccatgcgc gcgacctgcg cgttgggctc cggcggcgcc
    14881 aagttgccgg cgccgacggt ggcgaagccg ttgacctcgt cgacgatgac caggccgacc
    14941 cgccccgccg gcgcgaagcg ctcgcgcgcg agcggcagct gggtcgagag cgccgccagc
    15001 gccgcgcccg aatcgccggc caatgcgatg tcgttcacga tcgcctccct ggctgtgtcc
    15061 tcgccacgtg gggaacctcg ccgcgcctgt caacgtggcg ggcgaaggtc acaggtcggc
    15121 gggcggtgca acgccgctcc ggcggagctg gtcggcgagc gcggccttca gccggccgag
    15181 cccggcggca tcgcgcgcct cggcgcgcgc gaccagcacc gcctgggtgt tggacgcccg
    15241 cagcagccac cagccgtcgg cggtgttgac gcgcacgccg tcgaccgtga tcacctcggc
    15301 gccggccgcg gcgaggcggg cggcgacctc gtcgatcacc gcgaacttgc gcgcctcctc
    15361 gcacgggaag cggagctcgg gcgtgttgac catctgcggc agggcatccc gaagctcggc
    15421 cgcgctccgg ccgctgcgcg cgatcacgtc gagcaggcgg acgcctgcgt acaggccgtc
    15481 gtcgtagccg taatagcggt cggcgaagaa gatgtggccg ctcatctcgc cggccagcgg
    15541 cgcgccgacc tcggccatct tgctcttgat cggcgaatgg ccggtgcgcc acatcagcgg
    15601 caccccgccg agggcggcga tctggtcgaa cagcacctgg ctcgccttga cgtcggcgat
    15661 cacggtcgcg ccgggccgcg cggcgagcac atcggcggcg agcaggcaca ggatctggtc
    15721 gccccacagg atgcggccct ggccgtcgac cacccccagc cggtcgccgt cgccgtcgaa
    15781 ggcgaggccg agatcgagcc cctgcccggt cacggcctcg atcagcagct cgaggttctc
    15841 gggcaccgtc gggtccgggt ggtggaccgg gaagtcgccg tcgaccgtct cgcacagcag
    15901 cacgtggcgc ccgggcaggc gcgcggtcag gcgcaccatc gccgggccgg cggcgccgtt
    15961 gccggaatcc caggcgacgg cgagcccgcg gtcggcgcga taggcgccgg ccagcacgtc
    16021 gatataggcc gccatcacgt cgcgctcggc gaccgtgccg ggccggtcgt cgaacgcgcc
    16081 ggcggcggcg acgcggccga gctcctggat gtcgtcgccg aagaacgggc cggaggcgag
    16141 cgtcatcttc aggccgttgt gcgccggcgg gttgtgcgag cccgacacca tgacccccgc
    16201 ctcggcgccg agcgacttga ccgcgaagta gagcagcggc gtcggcccga ggccgatccg
    16261 ggtcaccgcg gccccggact cgcacaggcc ctcgaccagg gcggactcga gcgcgggcga
    16321 ggaatggcgg ccgtcatagc cgacgcagat gcgcgcgccg ccggtgcggg cgcgcaccat
    16381 ggtcgcgaac gcgcgcccga ccgcgcgcgc gtcgcccgca tccagcgtct cgccgaccac
    16441 gccgcggatg tcgtaggcgc gcagcgtggt cggattgagc gcgtgtcccc ggctcatcgc
    16501 gcggcccgcc cgcggccgat gccgacatag tcgaagccgg ccgcggccac ctcgtccggg
    16561 cggtagacgt tgcgcaggtc gaccatcagc gggcgcgcca tcagcgccct gagccgggcg
    16621 aggtcgaggg cgcggaactg gttccactcg gtcatgatga ccagcgccac cgcgccctcg
    16681 gcgatgtcgt aggcggagct gccgaaggcc actcccggca gcagcgcccg cgccgcgtcc
    16741 atgcccgccg ggtcgtaggc ccgcacggtc gcgcccgcct tttggagcgc cggcacgatg
    16801 accaggctcg ggctatcgcg caagtcgtcg gtgttgggct tgaaggtgag gccgaggacc
    16861 gcgatcgcct tggccgcgac cgagccgccg caggcgtcga tcaccttggc ggccatcgcc
    16921 gccttgcgcg cctcgttgac cgcggtcacg gtctcgacca ggtgcagcgg cacctggtgc
    16981 tcgcgcgcgg tgcgcgccag cgccagcgcg tccttgggga agcagctgcc gccgaagccc
    17041 gggcccgggt gcaggaactt ggcgccgatc cgccggtcga ggccgatgcc cttggcgacc
    17101 tcctggacgt cggcgccgac cttctcgcac aaatccgcga tctcgttgat gaaggtgatt
    17161 ttggcggcga ggaaggcgtt ggccgcgtac ttggtcagct cggcggcctc gcggctggtc
    17221 agcacgatcg gcgtctcgcg caggaacagc ggccggtaga gcgcgccgag caccgcgcgc
    17281 gcgtggtccg aggcggcgcc gaccaccacc cggtcgggat gcatgaagtc ctcgatcgcc
    17341 gagccctcgc gcaggaactc ggggttcgag gcgacgtcga agtcgacccc gggcgcaagc
    17401 cccggccggg cggcggcgat cacgcgctcg acctcgctgc cggtgccgac cggcaccgtc
    17461 gacttggtga ccaccaccgt gcgcaccgcg atcgcgcgcg cgatctgctc ggcggcggcg
    17521 aacacgtagc tcaggtcggc atggccgtcg ccgcgccggc tgggggtgcc gacggcgatg
    17581 aacaccgcat cggccgcggc catcgcgggc gccagttcgg tggcgaagcg gagccggccg
    17641 gcggcgacgt tgcgcgcgac cagggcctcg agcccgggct cgtagatcgg cagccggccg
    17701 gccgcgagtg cggcgatctt gtcgcggtcg gaatcgatgc aggtgacgtc gaccccgaac
    17761 tcggagaagc aggcacccga gaccaggccg acatagccgg tgccgaccat gacgatgcgc
    17821 atcgcccgcc tactcctgcc cggccaggcg ccggatgatc gcgcgcagct cggtgccgag
    17881 gtcgtcgcgg gcgagcgcga aggcgagcgt cgcctcgatg tagccgagct tgtcgccgca
    17941 gtcgaagcgc cggccggcga accggaagcc gtggaacggc gccttgccga tcagcccggc
    18001 catcgcgtcg gtaagctgga tctcgccgcc ggcgccccgc tcctggcgcg ccaggcggtc
    18061 catgatcgcg ggctgcagga tgtagcggcc gatgatcgac aaggtcgacg gcgcaacctc
    18121 gggctcgggc ttctcgacca gcccggcgac ctcgaccagc gcgccgcgcg tcgcgcccac
    18181 ctcgaggatg ccatagcggc gggtgtgctc cggcggcacc tccatcaccg cgaccaggtt
    18241 gccgccgacc cgctcatagg cggccatcat ctgggcgagg cacggcgact cgtgcagcac
    18301 caggtcgtcg ggcagcacca ccgcgaacgg ctcgttgccg acgaattcgc gcgcgcacca
    18361 caccgcgtgg ccgaggccga ggggctcctg ctggcgggtc gtgaacagtg cgcccggggc
    18421 aaggcgggcc gaggcggcga catcgaccag cgcccctttg ccggcgtcgg cgagcgtgcg
    18481 ctcgagctcg taagcgtggt cgaagtggtc ctcgatcagc gacttgccgc gcccggtgac
    18541 gaacacgaag cgctcgatcc ccgccgcctg cgcctcttcc accgcgtact ggatcagcgg
    18601 cttgtcgatc accggcagca tctccttggc cagcgccttg gtcgccggca ggaagcgcga
    18661 gccgagcccg gcgatcggga acacggcggt tctgaccggc gccgccatcg cttaagctgg
    18721 cctggcgacg tcgtcgacca cgagctggac ctcgctgcgc ccgcgccagc ggtcctcgcg
    18781 cagatggccg gcgacgtgca gcgcgccgcc gcgcgccgcc agcaccgcct ggccgaccgg
    18841 ggcgtcggcg gcgcgaaacg cgatcgcctt gaggctgccg ccgcccgccc cggccagccg
    18901 gcagcggaca tggccggcgc cgaccacatc ggcaaaggca atgcggcagt cgggcagcgc
    18961 gaaacgcggc tcagggttgc cgctgccgaa cggcccggcc tcgtcgagca ggccgcacag
    19021 ctcgaccgtc gcgccggcga tggcgagcgc gccgtcgagg ccgaggctcg gcgcccgctg
    19081 cgcgtcggcg cacgcggcca cgcgccggtc gaggaacgcg gccagcgcat cgatcccgtc
    19141 cgccgccgcg gtcagcccgg cggccatcgg gtggccgccg ccgtcgacca ggagccccgc
    19201 ctggcgcgcg gcgatgaccg cggcgccgat gtcgacgccg gcgatggagc gcgcgctgcc
    19261 cttgcccacc ccgccgtcga cggcgatcac gcaggcgggc cgcgcgaacg cctcgaccag
    19321 gcggctcgcg accagcccga tcacgcccgc atgccaaccc gcgcccgcga ccaccagcat
    19381 cgcccgcccc tcgcccgcct ggacctcggc gcgctcgagc gcctgcgcca gcaccgccga
    19441 ctcgatcgcc ttgcgctcgt cgttgaggcg gtcgagcgtc tgggcgatcg gcagcgcctc
    19501 ggcggcgctg cggctggtca gcaggcgatg gccgaggtcg gacttgccga cccgcccgcc
    19561 ggcgttgatc cgcgggccga gcacgaagcc tgcgtgccag gcggtcgggg cgcggtcgat
    19621 gcgggcgatg tcggcgagcg tggccaggcc cgggttcgcg cgcctggcga gcaccttgag
    19681 cccatggcgc acgaacgccc ggttgaggcc ggtaagcggc acgatgtcgc acacggtccc
    19741 gagcgcgacc aggtcgagcc ggtccctgag atcgggctcg gcgcgcgcgg cgaaatgccc
    19801 gcgcgcgcgc agctcgcggc tgaccgcgac cgcgagcatg aacgcgacgc ccaccgccgc
    19861 cagcgcgccg aggccgctct cgtcgtcgag ccggttgggg ttgaccaggg cgtgacaggc
    19921 cggcaggtca ggcccggatt gatggtggtc gacgacgatc acgtcgagcc ctcgcgcggc
    19981 tgcgtcggcg agcgcgccat gcgcggtggt gccgcaatcg agggtgacca ccagcgtcgc
    20041 accctcgccg gcaaggatcc tgagcgccgc gacgctcggg ccgtagcctt cgcgcacccg
    20101 gtcgggaatg taagggcgga cgcgcgcgcc gagcgcctcg agatggaggc ggatgagcgc
    20161 cgaggcggtg gcgccgtcga cgtcgtagtc gccgaacacg gcgatcagct cgccgccctc
    20221 gaccgcatcc gcgatgcgcg cggccgcgcg gtccatgtcg cgaaggccgg acgggtcggg
    20281 catcagcgcg cgcaaggtcg gatacaggaa cgcctcggcc tcgtcgaggg ccaccttgcg
    20341 cgccgccagc acccgcgcca gcatctcggg cacgtcgagt gcctgggcga gggccagcgc
    20401 caggccgtcg tcgtcgaccc ggctccgcca gcgcaggccc gcgaccgagc gctcgacgcc
    20461 gaggaaggcc ggtgcggcgc caaccgctgc catgctagcg ggtctcttcc ccgagcatcg
    20521 ccttgcgggt gaagtcgtgg ctggcgctga tcacccgcac cgtgcccgtc ttcgagcgca
    20581 tgaccaggga atcggtgctg gcaccgccgg cgtagcggcg cacgccgcgc agcatcgagc
    20641 cgtcggtgac cccggtggcg gcgaacatca cctcgccacg ggcgaggtcg gtgagggtat
    20701 atttgcggtc caggtcgtcg atgccgagat tgcgcgcgcg cgccctctcg tcgtcgttgc
    20761 ggaacaccag ccggcccatc atctggccgc cgatgcagcg cagcgccgcc gccgccagca
    20821 cgccctcggg cgcgccgccg ataccgaggt agatgtcgat accggtgccg gcgcgcgagg
    20881 tggcgatgac gccggcgacg tcgccgtcct ggatcagctg gattcgcgcc ccggcctcgc
    20941 gcaccttggc gatcagctcc tcgtgccggg gccggttgag gatgcacgcc accaggtcgc
    21001 tcaccggcac gcccttcgcc ttggccaggt tgcccaggtt ggcggccggc gcctggtcga
    21061 ggtcgacgac gccgtcgggc aggccgccgc cgaccgcgat cttgtccatg tagacgtcgg
    21121 gggcattgag gaagccgccc tgctcggcca tcgagatcac cgacagcgcg ttcgccccgc
    21181 cggtggcgca gatcgtggtg ccttcgagcg ggtcgagcgc gatatcgacc ttgggtccgc
    21241 ccgagccgac cttctcgcca atatagagca tcggcgcctc gtcgcgctcg ccctcgccga
    21301 tcaccaccgt gccgtccacg gccagcccgt tcagcgcccg gcgcatggcg tcgaccgcgg
    21361 cctggtcggc gaccttctcg tcgccgcggc ccatgtagcg cgagcaggac agcgccgccg
    21421 cctcggtcac ccgcaccacc tcgagcgcga ggttgcggtc gagtgtcgcc gtctccgcca
    21481 tctcccatcc ctccgccagc ggggtcgcgc cgagcacctt acaacgattc gatgcggatc
    21541 atcctgggcg gctcgcacac gctctcgagc gccgcgatct gcctgagcgc cgcgccgatc
    21601 gaggcctcga tcacgtcgtg ggtggtcatc accaccggca ccgcctcgtc gggcttgcgc
    21661 gcacgctgga tcatcgcctc catcgacacg tcgtggtcgc gcaggcaggc ggcgatgtcg
    21721 gcgatcacgc ccggccggtc gaccaccatc aggcgcacgt aataggggcc ggtgcggcgc
    21781 tcgatcggca ccgcggcgag ctccaccagc tcggcggcgg ggaccccgaa ggtgggcacg
    21841 cgctggccgc gggcgatgtc gaccaggtcg gcgaccaccg cggacgcggt cggcccggcg
    21901 ccggcgcccg ggccctcgaa caccgtcgag ccgatgaagt tgccctcgcc gacgaccgcg
    21961 ttcagcaccc cctcgaccgc cgcgatcggc gccgcggagg gcaccatgca cgggtgcacg
    22021 cgctgctcga ggccggcttc gagctggcgc gcgatgccga gcagctttat gcggtagccg
    22081 agatcgtcgg cgaacgcgat gtcgaccggg ctgacgtggc ggatgccctc gacatggacc
    22141 gagtcgaagt cgatccgggt gccgaaggcg aggctggcga ggatcgcgag cttgtgcgcg
    22201 gcgtcgatgc cgtcgacgtc gacgcccggg tcggcctcgg cgtagcccag cgcctgcgcc
    22261 tcggcgagca cctcggcgaa gtcgcgcccg gtctcgcgca tggtcgtcag cacgtagttg
    22321 caggtgccgt tgaggatacc gtagacgcgg gtcaggcggt tggcgaccag gccgtcgcgc
    22381 agcgccttga tcaccgggat gccgccggcc accgccgcct cgaagccgag cgagacgccg
    22441 ttcgcctcgg ccagcgcggc gagctcgccg ccgtgatggg cgatcatcgc cttgttcgcc
    22501 gtgaccaccg gcttgccgcg cgcgagcgcg gcgcggacca gcgccagcgc cgggccgccc
    22561 gagccgccga tcagctcgac caccgcgtcg cagtcggcct cggccagcgc ttcggcctgg
    22621 tcgacccagc gcgcctcgcc caggtcgacg ccgcgcacct tgccccggtc gcgggccgag
    22681 accgcgacca cgtcgatcgc ccgcccgcag cgctggcgca acagctcgcg gttggtctgg
    22741 agcaggcgca cggtgccgac gccgaccgtg cccaggcccg cgatcgcgac tcgcagcggc
    22801 ctggtcaaga ggcggcggcg cgccgcggcg cctcgggcac ctcgaccgtg gcgaggaacg
    22861 ccttcaggtt gcgcgccgcc tggcggatgc gctggcggtt ctcgaccagc gcgatgcgca
    22921 catggccctc gccgtactcg ccgaagccga tgcccggcga gaccgcgacc tcggcatgct
    22981 cgagcagccg cttggagaac tcgagcgagc cgactggctg ccaggccgcc gggatcggcg
    23041 cccacacgaa catggtggcg accggcttgg gcagcggcca gccggcggcg gcgaagctgt
    23101 cgatgagcac gtcccggcgc tcgcgatagc gcgcgcgaat ctcgtcgatg cattcctgcg
    23161 ggccgttgag cgccgcggtc gccgcgacct ggatcggggt gaaggcgccg taatcgagat
    23221 acgacttgat ccgcgccagg gcgccgatca ggcgcgcatt gccggcggcg aatccgatcc
    23281 gccagccggg catcgagtag gtcttgctga gcgaggtgaa ctcgaccgcg acctcgcgcg
    23341 cgtcgggcac ctcgaggatc gacggcggcg gcgcgcactc gaagtagatc tcggcatagg
    23401 cgaggtcgga caggacatag atgccgtgct tgcgcgcgat cgccaccagc cggcggtaga
    23461 agtcgaggtc gaccacctcg gcggtcgggt tgttcgggta gttgacgacc accgccttgg
    23521 gcgtgcgcgg gcaggccgcc accgcgcgct cgagctcggc gagcacgtcg accccggggc
    23581 cgctcggcac atggtggacg gcggcgccgg cgatgacgaa gccgtaggga tggatcggat
    23641 agctcgggtt ggtcgccagg atgatgtcgc ccggcgaggt catcgccgag gccaggttgg
    23701 cgagaccttc cttggagccg agggtgacga tcacctcgcg ctcagggtcg agctcgactc
    23761 cgaaccggcg ccggtagtag gcggccatgg cccggcgcag ccccgggatg ccgcgcgaga
    23821 ccgagtagcg gtgggcgcgc gggtcgcgca cggtctcgac cagcttctcg acgatgtggc
    23881 gcggcgtcgg gctgtcgggg ttgcccatgc cgaagtcgat gatgtcgcgc cccgcggccc
    23941 gggcgcgccg tttcatctcg ttgacctcgg cgaacacgta gggcggaagc cgacggatac
    24001 gttcgaactc gtctgtcata gggtcatcgc cggaaacgcc gcacgaaagc ggccggggcg
    24061 ggcgttgctt acccgccttg ccgctagaaa tcgatatata tgtcggcgcg ccggttcgcc
    24121 gcctcgccga gcttggtcgt ctccgaatag gccggggcgt cgctcgacat ggagctcggg
    24181 aagatctggt cggcgggcac gccctggcgg gcgagctcct cggccaccat gcgcgcccgg
    24241 tcgaaggcga ccgcgaagtt ggcgagcttc gccttgacct cgtcggtgcc gtcgacgcgg
    24301 ccgctggaat ggccgaccac gcgcacccgc ccgccgcgcg accggtagag gtccgcgatc
    24361 tggccgagca cctgcgtgtc ggccgccgac aggctggtcg agccataggc gaagtagatc
    24421 accccggcat ggaccgagcc catcgccggg ccggacgcct cgaccggcag gggccgcgac
    24481 gccggggcgg cggccgaggc cagcaccggt gccgccgcgg ccacgcgcac ggacgcaggc
    24541 gcttcgagcg ccggggtcgc ggtcgcgacc ggcgccggcg gcgccatggt ctcgcgccgc
    24601 ggcgccgacg ccgccatcgg cacgggcgca gcctcgagcc acggcattgc ctcggtctcg
    24661 actgccgtcg cgggcatcgg catcggcgcg gacgcgaccg ggcctgccgc gggcacgggc
    24721 atcggctcag gcaacgtctc gggctcgggt tccgccgccg ccatgggcgt cggcgcgggc
    24781 attggcgcgg cctcagtgac cgtgaccgcc ggcgccggcg caccggccgc gatctcgggc
    24841 tcgggcgcgc gctcgactgc cggggcggtc ggctcgcgcc aggccagcgc gggctcgcgc
    24901 atcaccgcct ccgccgccgg gttgtcgaca taacgcgccg cagcccggtc cgcgaccagg
    24961 ccctgggcga tctgctggcg ctcgtcatag gcggtcacct tgggcgcctg gccgggcacc
    25021 gcgccgaggc tggggaacgg ctcgtccgct cccggaacgg gctccgcctg ctcctgctcc
    25081 gcgcgcgccg caagctcggg gtcggcggcc tcgtcctcgc cactcaggta ggaatccgcc
    25141 gagctcaccc attcgaccgg gttgagcgcg tccggcatcg ccgagcagcc ggcgagcgcc
    25201 agcccgaaca ggccgccgag cgcccaaacc ctgaccggga cggcgacggc cgatgcgact
    25261 tgatggactg agcgccaagg gctaagtaaa tccatgaatc gcgtgtgctc ctgtgttccg
    25321 aggcacgcgc cgcggcgacc aagcgcaggc ggcggctgtc aagggcccct ccgcccggct
    25381 gccgctggag ggcggcgcgt caccggcgga agtgcaacgg tttcagtcta tcgcaaaggc
    25441 tgttttccac aagccggcag ccgtgccggg gaccggggcg gcggctgatc gcagccgatg
    25501 cggcggaaca cgagagagga cgccgccgcg accatgaccg agacggacag ggaaccggat
    25561 accgccgcgg ggacgctgca ggcggccgag acttggcagc ggatcgccga gcgcagccag
    25621 cgcatcgtgc aacgcttcct cacccgccac gccggcgagg agacgccgtt cggcgacccc
    25681 gccaacctgg tcggcgcgtt cgccgagatg accggccggc tgttcgccga cccggcgcgc
    25741 ctgatgcagg cgcagatgga cctgtggcag agccacatgc ggctgtggac caacaccgcc
    25801 gagcgcctgc tcggcagccg ccggcccgag ccggtggccg cgccccaacc cgaggaccgc
    25861 cggttccgcg accaggcgtg ggaagagaac gcggtgttcg actacatcaa gcagtcttat
    25921 ctcgtgaccg cgcgctggct gcagtcgacg gtccgcggcg tcgagggcct cgacgacaag
    25981 accgcgcgca aggtggactt ctacactcgc cagttcgtcg acgcgatggc gccgaccaac
    26041 ttcgtgctga ccaaccccga ggtgctgcgc gagacggtcg acagcaaggg tgagaacctg
    26101 gtgcgcgggc tcgagaacct gctccacgac ctcgagcgcg gcgacggcga gctcaggatc
    26161 tcgatgaccg acatggcggc gttcgaggtc ggtaagaacg tcgccgtcac ccccggcgag
    26221 gtgatgtttc gcaacgacat gatcgagctg atccagtacg cgccgaccac cgcgacggtg
    26281 cacaagcgcc cgctgctggt ggtgccgccg tggatcaaca agttctacat cctcgacctc
    26341 aggcccagga actcgttcgt caagtgggcc accgaccagg gctacacggt gttcgtcatc
    26401 tcgtgggtga accccgaccg caagctcgcc gagaagacct tcgacgacta catgctggag
    26461 ggcccgatcg ccgcgctgga tgcgatcgag caggcgacgg gcgagcgcga ggcgtgcgcg
    26521 gcctcgctgt gcctgggcgg caccctgctc gcctgcacgc tcgcctacct ggccgcgatc
    26581 ggcgatacgc ggatcaagtc ggcgaccctg atggcgagcc tggtcgactt ctccgagccc
    26641 ggcgagctcg gcgtgttcat cgacgacgag cagctcgcag cgctcgagac gcgcatgcgg
    26701 cagaagggcg gctatctcga cggcggcgcc atgtcgacca ccttcaacat gctgcgcgcc
    26761 aacgacctga tctggtcgtt cgtgatcaac aactatttgc tcggcaagga gccgctgcca
    26821 ttcgacctgc tgtactggaa cgccgattcg acgcgcatgc cggcgcgcat gcactcgttc
    26881 tatctgcgca acatgtacca gaataaccgg ctcatccagc cgggcgggat cgagctcgcc
    26941 ggcgagaaga tcgacctcgg caaggtcaag actccggtct acgtgctggc gaccagggag
    27001 gaccacatcg cgccctggcg ctcggcctat gccgcggtgc acctgttcgc cggcccggtg
    27061 cgcttcgtgc tcggcggatc gggccacatc gcgggcatca ccaacgcgcc ggcggtggcg
    27121 aagtatccgt actggaccaa ccgccgcaag cccaagtcgc ccgacgcctg gctcgactcg
    27181 gcgagcgcgc accagggctc gtggtggacc gactgggaca agtggcaggc gcgccacgcc
    27241 gggccgttgg tgccggcgcg cacccccggc accgggcggc tcgcgtcgct cggcccggcg
    27301 cccggtgcgt acgtgctgca gaaggagaag tgagccgcca tggcgctccc caacactccg
    27361 cgcaagacgc tcgtgctgac cggcgccagc cgcggcatcg gccatgccac ggtcaagcgc
    27421 ttctcgagcg ccggctggcg ggtgatcacc tgctcgcgga tggcgttccc ggagggctgc
    27481 ccgtgggagg cgggcccgga ggaccacctg cagatcgacc tcggcgactc cgacagcctg
    27541 gacgagcgca tcgcccagct caagcgcatg ctcgacggca gcccgctgca tgcgctggtc
    27601 aacaatgccg ccatctcgcc caagggcccg gacggcgccc gcctcgacag cctgtcgacg
    27661 ccgatggccg actggcacca ggtgttcggg gtcaacttct tcgcccccat ccgcctcgcc
    27721 cgcgggctga tcgaggagct caagaaaggc ccggggacga tcgtcaacgt cacctcgatc
    27781 gcgggcgggc gggtgcaccc gttcgccggc accgcctatg ccacctcgaa ggcggcgctc
    27841 gcctcgctca cccgcgagat ggcctacgac ttcggccgcc tcggcatccg ggtcaacgcc
    27901 atcgcgccgg gcgagatcag gaccgcgatc ctgtcgcccg acaccgaggc gcagatgctg
    27961 cccgacatcc cgctcggccg cct
//
LOCUS       DASWHU010000357         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_227888, whole genome shotgun sequence.
ACCESSION   DASWHU010000357 DASWHU010000000
VERSION     DASWHU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227888"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGB88_13295"
     CDS             <1..276
                     /locus_tag="VGB88_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX9648473.1"
                     /translation="GVAALVYTDIARDGALRGLDVAAVAEFARALTTPVIASGGIASL
                     ADIAALKAVADSGPVSEAGAGIVGAICGRALYDGRLDAAEALALLAA"
     gene            282..1043
                     /gene="hisF"
                     /locus_tag="VGB88_13300"
     CDS             282..1043
                     /gene="hisF"
                     /locus_tag="VGB88_13300"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9648474.1"
                     /translation="MLKVRVIPCLDVHAGRVVKGVRFVDLVDAGDPVEQAHVYDAAGA
                     DELCFLDITASAEDRDIILEVVARTADACFMPLTVGGGVRSTEDIRALLLAGADKVSI
                     NTAAVRRPELVAEAATKFGAQCITVAIDAKQAAPGRWEVFTHGGRNATGRDAVAWAKA
                     CAQAGAGEILLTSMDRDGTRAGFDLALTAAVADAVPVPTIASGGVGMLEHLVEGVRAG
                     HASAVLAASIFHFGEATIAEAKAHMQAAGVPVREV"
     gene            1326..1709
                     /gene="hisI"
                     /locus_tag="VGB88_13305"
     CDS             1326..1709
                     /gene="hisI"
                     /locus_tag="VGB88_13305"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9648475.1"
                     /translation="MTENVLDRIVFDRDGLVPVIAQSHASGEVLMLAWMSRDALAETL
                     ASGRMCYFSRSRGRLWRKGETSGQVQRLIELRLDCDGDALLALVDQTGVACHTGRRSC
                     FFTRLSEAGAETVAEVLVPPERLYG"
     gene            1702..2043
                     /locus_tag="VGB88_13310"
     CDS             1702..2043
                     /locus_tag="VGB88_13310"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX9648476.1"
                     /translation="MAESPAAGPEVLARLAAVIEQRRSADPERSYVARRLAQGRHRIA
                     KKLGEEAVECALAAVDGAPETLVAESADLLFHLLILWAACGVAPEQVFAELARREGTS
                     GLDEKRARRAE"
     gene            2067..>2429
                     /locus_tag="VGB88_13315"
     CDS             2067..>2429
                     /locus_tag="VGB88_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX9648477.1"
                     /translation="MAYDDDNVFARILRGEIPADKVYEDEHVLAFNDIAHMAPVHVLV
                     IPKGKYVSMDDFAAKASDAEIAALVRAMGRIARQAGVAESGYRVVANTGPDSGQEVMH
                     LHFHVFAGRRLGGMRRQAG"
BASE COUNT          319 a          867 c          906 g          337 t
ORIGIN      
        1 ggcgtcgccg ccctcgtcta caccgacatc gcccgcgacg gcgcgcttcg agggctcgat
       61 gtcgccgcgg tggccgaatt cgcgcgcgct ctcaccacgc cggtgatcgc ctcgggcggc
      121 atcgcctcgc tcgccgacat cgccgcgctc aaggcggtgg ccgactcagg ccccgtctcg
      181 gaggccgggg caggcatcgt cggcgcgatc tgcggccgcg cgctctacga cgggcggctc
      241 gatgccgccg aggctctagc tctgctcgcg gcgtgaacgc cgtgctcaag gtgcgcgtca
      301 tcccctgcct cgacgtgcac gcggggcgcg tggtcaaggg ggtgaggttc gtcgacctgg
      361 tcgatgccgg cgacccggtc gagcaggcgc acgtctacga cgccgccggc gccgacgagc
      421 tgtgcttcct cgacatcacc gcctcggctg aggaccgcga catcatcctc gaggtggtcg
      481 cgcgcactgc ggacgcgtgc ttcatgccgc tcacggtagg cggcggcgtg cgctcgaccg
      541 aggacatccg cgccctcctg ctcgccggcg ccgacaaggt gtcgatcaac accgccgcgg
      601 tgcgacggcc cgagctggtc gccgaggccg cgaccaagtt cggcgcccag tgcatcacgg
      661 tggcgatcga cgccaagcag gccgcgcccg ggcgctggga ggtgttcacc catggcgggc
      721 gcaacgccac cggccgcgac gcggtggcct gggccaaggc ctgcgcgcaa gccggcgcgg
      781 gcgagatcct gctcacctcg atggaccgcg acggcacccg ggcgggcttc gacctcgcgc
      841 tcacggctgc ggtcgccgac gcggtgcccg tgccgaccat cgcctcgggc ggggtgggca
      901 tgctcgagca cctggtcgaa ggggtgcgcg cgggccacgc cagcgcggtg ctcgccgcct
      961 cgatcttcca tttcggggaa gccacgatcg ccgaggccaa ggcgcacatg caggccgccg
     1021 gggtgcccgt gcgcgaagtg taatttgtgc acgcggagcc gcggagggcg cggaagagcg
     1081 gagttcgtca tcgcgagcgg agcgaagcga tccagggccg gcgcaggccg ctccgagggc
     1141 tggatcgcct gcctgcgcgc agccgaagcg gcttcgcttc ggcgaaggca ggccgcggcg
     1201 cttcgcgcct cgcgatgacg gtgtctccgc gttctccgcg cctccgcgtg agtcgaccgc
     1261 cgggcgcgcg ggtgacaggg tggggcaaat ctgctaccgc tcgccgcagc ttgcggagcg
     1321 cctgaatgac tgagaacgtg ctcgaccgca tcgtcttcga ccgcgacggg ttggtgccgg
     1381 tgatcgccca gagccacgcc tcgggcgagg tgctgatgct cgcctggatg agccgcgacg
     1441 cgctcgccga gacgctggcg agcgggcgca tgtgctactt ctcgcgcagc cgcgggcgct
     1501 tgtggcgcaa gggcgagacc tcgggccagg tgcaaaggct gatcgagctc aggctcgact
     1561 gcgacggcga cgcgctcttg gcgctggtcg accagaccgg ggtcgcctgc cacaccggcc
     1621 ggcggagctg cttcttcacc cgcttgagcg aagccggcgc ggagacggtg gccgaggtgc
     1681 tggtgccgcc ggagcgcctc tatggctgag tcgcccgcgg ccgggcccga ggtgctggcg
     1741 cgcctcgcgg cggtgatcga gcagcgccgc tccgccgacc ccgagcgctc ctatgtcgcc
     1801 cggcgcctcg cccagggccg ccaccggatc gccaagaagc tcggcgagga ggcggtcgag
     1861 tgcgccctcg ccgcggtcga cggcgcgcct gagacgctgg tcgccgagag cgcggacctc
     1921 ttgttccacc tgctcatcct gtgggcggcg tgcggggtgg cgcccgagca ggtgttcgcc
     1981 gagctcgccc ggcgcgaggg cacctcgggg ctcgacgaga agcgcgcgcg gcgggcggag
     2041 tagcccgcat cgagcggagg gcggcgatgg cctatgacga cgacaacgtg ttcgcccgca
     2101 tcctgcgcgg cgagattcct gccgacaagg tctacgagga cgagcacgtg ctcgccttca
     2161 acgacatcgc ccacatggcg ccggtgcacg tgctggtgat cccgaagggc aagtatgtgt
     2221 cgatggacga cttcgcggcc aaggcgagcg acgccgagat cgcggcgctg gtgcgcgcca
     2281 tgggccggat cgcgcgccag gccggtgtcg ccgagtccgg ctaccgcgtg gtcgccaaca
     2341 ccggccccga ctcgggccag gaggtgatgc acctccactt ccacgtcttc gccggccgcc
     2401 gcctcggcgg catgcgccgc caggccggc
//
LOCUS       DASWHU010000358        11455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2472185, whole genome shotgun sequence.
ACCESSION   DASWHU010000358 DASWHU010000000
VERSION     DASWHU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472185"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VGB88_13320"
     CDS             complement(<1..866)
                     /locus_tag="VGB88_13320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9648478.1"
                     /translation="MTPARLRSGAWFARAPVRALSAALVLVYLAALAAYALTGDGLAD
                     YKGRPLGTDFVSFWAAGRLALDGAPEAAYDLDRHAEAERRAIGRDTLPYYAWHYPPIF
                     LLLMLPFALLPYVWSLALWLAATLAAYLAVAARLSRDRLAIWLALAFPAAFVNLIHGQ
                     NAFLSAALVGGALHLLRTRPAAAGALIGVLAFKPQLGLVVPFALAAGRHWRCFAAAAA
                     TVAALAGASVLAFGWPTWQAFAANSGFAWDVTLVQGAAGWPKLQSAFAATRLLGGPAW
                     LGAVVQAAVSVAA"
     gene            complement(1026..1355)
                     /locus_tag="VGB88_13325"
     CDS             complement(1026..1355)
                     /locus_tag="VGB88_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX9648479.1"
                     /translation="MVREIASGEPRESSLSRNARYLPDLRAQMRNVTVLPPPVSGGAP
                     PGPGGPMIRVLRQFRRNRSGATAIEYGLVAAGISVAIIGALTLVGSEIQSTFTTIAAA
                     VDNAVQN"
     gene            complement(1506..3539)
                     /locus_tag="VGB88_13330"
     CDS             complement(1506..3539)
                     /locus_tag="VGB88_13330"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648480.1"
                     /translation="MIRCRGSIAAALVALVLAGPAWADLDAVKAALDRGDSEAAAEQL
                     QPLAEAGDAEAQTLLGKLYLKGTGVPANYSLAWSWFERGANGGNGEAQYQLGEMHWNG
                     VGVPLNEHEAVKQYQAAADQGHEDAQLVLGLIYRDGRRQIPASPAKAHHYLRLAADQG
                     NPEAVLALEEMFVSGTAPADAQQEFSSERETQALTEAERIRAALIQWIEFVNRAGGGQ
                     ALRIGPDVVVAETADGFSVMIPDLVLTPESGAVIRAGTLRIKLTPQGELPEEPEQDLM
                     ADRRYQVEATLPDRIQIQQNLETYNLTIAASELTGLWLPALQTFAEGHMVLDGLALVD
                     QAGHTLFTAGQAKSEIDLKETEPGMWSGPSTFELAEIAVFDRGGPQRLSVGRVYGGNR
                     FRGLRLEDNRQLTMQMQTDPAGFFAGLLSGQIGDSWVKTLTGMMTYTAFEMGIEKLVV
                     YDETGDAAMGGLGRMTFSAEANQEDAGGGLLRLAYGHDGLDVGDQVPGTGGALVPTKA
                     NLDLTLDHLPVEMLVRLMIETFGHMMVESAQAGEDPAQPPPGAMMAESTAKLSQALSE
                     ARTGLSVTLSLDTEASPLRLGGLLVAQSDAAYGAAGDFELSIGNLDQIMALIDAEPTL
                     VAYLPVIQAFAGVAERSGDQGQAAKFRIAVQPDGAVLVNGHDAIELSVGGTAE"
     gene            3821..4477
                     /locus_tag="VGB88_13335"
     CDS             3821..4477
                     /locus_tag="VGB88_13335"
                     /inference="COORDINATES: protein motif:HMM:NF022607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEX9648481.1"
                     /translation="MGVRLFLILVLLVLAGGYLGWPYYTLYRIDVALAGNDTATLDRL
                     VDWGSVRAGVERELAARNVSREDTGAGASGERPGGLAGLVVPRFGQMLVDAYASPRGL
                     ARLMRDARVAPPAARASARAGAGTGAPAPFRPALFEAISYARDKVAAAEALACATYRR
                     LRDDYDYLFFTNPFTFEARYAPRAADQTEPLILTLRFQDLIWRLDRVDLPSARAGDGG
                     "
     gene            complement(4542..5819)
                     /locus_tag="VGB88_13340"
     CDS             complement(4542..5819)
                     /locus_tag="VGB88_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648482.1"
                     /translation="MSYILNALRKSEQERQATGRPPLPVRLAAAGGLKPARARRRWPW
                     LAAGAGAAAVAAAGAVLVLGRGEAPDGGREVVAVAEAVGEPATMPALLVATVDPVRPS
                     ADSPTTLAAARLAPAPAPPPAEPQASPAIPDQQVPQGVGPLSVPSLPLAELVPPPPNV
                     AAGTPPPPVVAQAPAVVAALPAPAGQEPLPRPTTDREAIAKSAQLNELGWDRERDDRY
                     DSAIDAFTRALALRPDYAEAYFGRAWANERLDRLDQAIADYGQAVRLKPDFATAYNSR
                     GIALLYRGRHAEAGNDFATAFDLGDGELKRYAMLWRYIAFERDGQDAPAQLAADARAV
                     DLDRWPGVLARYYLGESSTDAVLAEAESKDPITRRERLCVAYFFLGQRALLRGDPSHA
                     KDYFRSTLDTGVTAFAQYAAAERELLRLTSVRE"
     gene            complement(5860..7752)
                     /locus_tag="VGB88_13345"
     CDS             complement(5860..7752)
                     /locus_tag="VGB88_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9648483.1"
                     /translation="MFWEYLGFDENPFSIAPDPRYLYMSKRHQEALAHLVYGIGEGGG
                     FVLLTGEVGTGKTTLCRSLIEQLPDGVDLAFVLNPRVNEIELLATICEEMGVPYAREN
                     PSLKLLVDRLNQHLLAANARGRQPVLLIDEAQNLSPGVLEQVRLLTNFETTTRKLLKI
                     VLIGQPELRDVLRRPGLRQLDQRITARYHLEPLSAAETAGYVRHRLEVAGATREIFTP
                     RAIREVHRRSGGVPRLINTLCDRALLGAYGQDRSVVDRRLVRGAASEVRGRDPQAGRG
                     RWRFAAAAATVAIAGAVVAADRYGLRSWPGLDPGPGPGPVSEAAATTAAAEPVLDDVF
                     VTAAGPTTAALPGVGSDPGAAATPPAEVPPSPAAGFTVARLPAPPISEPPAAMIAVAM
                     LPPPWPSGPAGATADEAVVMAAVTPPSATRPAPVSLEDRLSGPGSTGDFETAVADLFA
                     VWGFDYEELTGMTACRKARAVNLLCQEGSTDWHQVRIADLPVVMTLHLADGRRVHLAL
                     TGLGDGSATVIVGGEQVETTVATLSALWTGDYLMLWRPPYNFERQLKPGVKGEDVVWL
                     QNRLAELRGAPPAKAARAVFDDSLAAQVRMFQRDHELEVDGVAGPRTMIRLSQTMPGY
                     RAPSLR"
     gene            complement(7957..9078)
                     /locus_tag="VGB88_13350"
     CDS             complement(7957..9078)
                     /locus_tag="VGB88_13350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9648484.1"
                     /translation="MHYTDHPDEVAEEVFAGLSFMAQHNVPTNPSNIAVWYAYAAGRY
                     PDLCRTIDEAIAERRPFTEDLCAEIYDKYFSMARESALVEDTSERIVGELRRAQQYAA
                     AANQRTNEISDRLQQYSAQLSGNEAAVGVKSMIDNLVAEAHSIDSETGELASNLGEVA
                     GEIGAVVDRVLTDSRSLTDEARKLADKLENTVFEIKEIERDFRGVREETLTDALTGVR
                     NLKFFHLALKIAVDRAREDQGQLSVCVLDLDHFEQFNRSYGRTVGDQVLRLVARTIVE
                     CVKGQDAIGRIGGEEFAIMLPETAKDGAVKVAENVRAAFGRKTLTNRKTGERYGTITL
                     SVGVTQYRNGESGSELLRRATTAMMSAKESGRDRVVVEP"
     gene            complement(9242..9466)
                     /locus_tag="VGB88_13355"
     CDS             complement(9242..9466)
                     /locus_tag="VGB88_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648485.1"
                     /translation="MAVLAACVLIGWALFQFAHDHRIDNVVSEWARSVYVTIQVMFRM
                     DPMLTLILFPLGIVIVLVLTGLLIWIQRER"
     gene            10053..>11455
                     /locus_tag="VGB88_13360"
     CDS             10053..>11455
                     /locus_tag="VGB88_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9648486.1"
                     /translation="MQRLADRDFDGIADSLAERLQRLAPADQVDVIVTFAARRTAADA
                     RAQIGPFTLKREFRIINGFAARMPGALVRRLASMPGIARIEEDFEVHAVLDIVTRDTG
                     ASDARADFAVTGFGTTGCVVDTGVDPNHEQLDSKTIGPADFADFINSRTNPYDDHWHG
                     THVAAIIAGDGTGTSASAQRFRGFAPDADIVAAKVLNSNGSGLASQIIAGIEWCASRA
                     DVDVINLSLGGGPTDGLDAMSRAVNCATDPTWSTSCGQVNGHPKVVLVAAGNSGAAPS
                     TVSTPGTAELAITVGAFADWSGSPGSGGQDDGLYLRSFSGRGPVKTGAGAVRYVKPDL
                     AGPGSRVGSAYSANGPTGYAFASGTSMATPSIAGIVALMIEANDVYSDGLLMTADANG
                     LPADKVRQIFELTSTDLGPPGLDNEYGYGVVDAYAAIAEAAGQSYDPTPLPQHLRLAA
                     QSVANGGSWTYGFSIGA"
BASE COUNT         1605 a         4142 c         4057 g         1651 t
ORIGIN      
        1 gcggccaccg acaccgccgc ctgcaccacc gcgccgagcc acgccggccc gcccaagagg
       61 cgcgtcgccg cgaacgcgct ctgcagcttg ggccagccgg cggcgccctg gaccaaggtc
      121 acgtcccagg cgaagcccga gttggcggca aacgcctgcc aggtcggcca gccgaacgcc
      181 agcacgctcg cgccggccag tgcggcaacg gtggccgcgg cggcggcgaa gcaccgccag
      241 tggcgcccgg ccgccagcgc gaacggcacc accaggccga gctggggctt gaacgcgagc
      301 acgccgatga gggcgcccgc cgccgccggc cgggtgcgca gcaggtgcag cgcgccaccg
      361 accagggctg ccgacaggaa cgcgttctgg ccgtgaatca ggttgacgaa cgcggccgga
      421 aacgcgagcg cgagccagat cgcgagccgg tcccgggaca gccgtgccgc caccgcgagg
      481 tacgcggcca aggtcgccgc gagccacaac gcgagcgacc agacgtacgg cagcaaggcg
      541 aacggcagca tcagcagcag gaagatcggc ggatagtgcc aggcgtagta cggcagcgtg
      601 tcgcggccga tcgcccggcg ctcggcctcg gcatggcggt cgaggtcgta ggcggcctcg
      661 ggcgcgccgt cgagcgccag ccgcccggcc gcccaaaagc tgacgaagtc ggtgccgagc
      721 gggcgtccct tgtagtcggc gaggccgtcg ccggtcagcg cataggccgc cagcgcggcc
      781 agatagacca gcaccagcgc cgcgctcagc gcacgcaccg gcgcgcgcgc gaaccaggcg
      841 cccgacctga gccgcgccgg ggtcacgcgc gctgcccgcg cccggcggcg atccggcgtc
      901 gtgggcggcg aggacgcggc gcaggcaacg acatctggcc cggattgctt accatgacgc
      961 gggaggccgt cgcgccgggg acgctgcgcc cgtgtgccgg acgggcccgg ccggacccgc
     1021 cggcgtcagt tctggaccgc gttatcgaca gcagcagcga tagtcgtgaa agtggactgg
     1081 atctcggagc cgacaagggt cagcgcgcct atgatcgcga ccgagatccc ggccgcgacc
     1141 agcccgtact cgatcgccgt ggcgccggac cggttgcgcc gaaactgccg cagcactctg
     1201 atcatgggtc cgcccgggcc cggtggggcg ccgccggaca caggcggcgg caggacggtc
     1261 acgttccgca tctgcgcgcg caggtcgggc aagtaacgcg cattgcggct caggctcgac
     1321 tctcggggct cgcccgatgc gatttcgcga accattgctc ctcaacagcg cggcggccga
     1381 gagcgtggtc ccgcggccac agcattttcc ctatcctgtt gggaacatac cctaatatgt
     1441 cgaccgccgc aaggcggtcc gggccggcgc gctcgcggtc gaagcgggcg cgccggccgt
     1501 tggcgtcact cggcggtgcc gccgaccgac agctcgatgg cgtcgtggcc gttgaccagc
     1561 acggcgccgt ccggctggac cgcgatgcgg aacttcgccg cctggccctg gtcgccgcta
     1621 cgctcggcga ccccggcaaa cgcctggatc accggcaggt acgcgaccag cgtcggctcg
     1681 gcgtcgatca gggccatgat ctggtcgagg ttgccgatcg agagctcgaa gtcgccggcg
     1741 gcgccgtagg cggcgtccga ctgggcgacc agcaggccgc ccaggcgcag cggcgaggcc
     1801 tcggtatcga gcgacagcgt caccgacagg ccggtccgcg cctcgctcag cgcctgggac
     1861 agcttggcgg tgctctcggc catcatcgcc ccgggcggcg gctgcgccgg gtcctcgccg
     1921 gcctgcgcgg actcgaccat catgtggccg aaggtctcga tcatcaggcg caccagcatt
     1981 tcaaccggca ggtggtcgag ggtgaggtcc aggtttgcct tggtgggcac cagggcgccg
     2041 ccggtacccg gcacctgatc gccgacatcg aggccgtcat ggccataggc caggcgcaac
     2101 aggccgcctc cggcgtcctc ctggttggct tcggcactga acgtcatgcg cccgagcccg
     2161 cccatcgccg cgtcgccggt ctcgtcgtag accaccagct tctcgatccc catctcgaag
     2221 gcggtgtagg tcatcatgcc ggtcagggtc ttgacccagc tgtcgcctat ctggccgctc
     2281 agcaggccgg cgaagaatcc ggcgggatcg gtctgcatct gcatggtcaa ctggcggtta
     2341 tcctccagcc gcaggccgcg gaagcggttg ccgccataga cgcgcccgac cgacagccgc
     2401 tgcggcccgc cgcggtcgaa caccgcgatc tccgccagct cgaaggtcga gggtccgctc
     2461 cacattccgg gctcggtttc cttgaggtcg atctccgact tcgcctggcc ggccgtgaac
     2521 agcgtatgcc ccgcctggtc gaccagcgcc aggccgtcga gcaccatgtg gccctcggcg
     2581 aaggtctgaa gtgccggcag ccacaggccg gtcagctcgg atgccgcgat agtgaggttg
     2641 tacgtctcga ggttctgctg gatctggata cggtcgggaa gggtcgcttc gacctgatag
     2701 cggcggtcgg ccatcaggtc ctgctccggc tcctcgggca gctcgccctg cggcgtcagc
     2761 ttgatccgca gcgtcccggc gcgaatcacc gcgccgctct ccggcgtcag caccaggtcg
     2821 gggatcatga cgctgaagcc gtccgcggtc tcggccacca ccacgtcggg accgatgcga
     2881 agcgcctggc cgccgccggc gcggttgacg aactcgatcc actggatcaa cgcggcgcgg
     2941 atgcgctcgg cctcggtcag tgcctgcgtc tcgcgctccg acgagaactc ctgctgcgcg
     3001 tccgccggtg cggtgccgct cacgaacatc tcctcgagcg cgagcacggc ctcggggttc
     3061 ccctggtccg cggccaggcg cagatagtgg tgcgccttgg ccgggctggc cgggatctgc
     3121 cggcggccgt cgcggtagat caggccgagc accagctgcg cgtcctcgtg gccctggtcg
     3181 gcggcggcct gatattgctt gaccgcctcg tgctcgttga gcggcacgcc gacgccgttc
     3241 caatgcatct cgcccagctg gtactgcgct tcgccgttgc cgccgttggc gccgcgctcg
     3301 aaccagctcc aggcgagcga gtagttggcg gggacaccgg tccccttcag atagagcttg
     3361 ccgagcaggg tctgggcctc ggcgtcgccg gcctcggcga gcggctgcag ctgctcggcc
     3421 gcggcctcgc tgtcgccgcg gtcgagcgcc gccttgaccg cgtcgaggtc ggcccaggcg
     3481 ggacctgcca gtaccagcgc caccagcgcg gcggcgatcg aacctctgca cctgatcacg
     3541 gcgaacctcc ggacaagcgt catcatcgcg gaccgatcat aggcggccgg caccggcgcc
     3601 gaaaggcccc gctcggcgca cgacccttcc tgatctcgac tccgtcccgt caatcaatat
     3661 ggggccgccg gcgaccctcg ccacgacccg atgcggggcg cgcgcttggc gttttggctg
     3721 ccgccgcgat agattcatcg ccgcgcgccc gcgtcggcgc ccggtccggc gcgttcgggc
     3781 gttggcccgc agcgccggga accgtgacgg gagacctctc atgggcgtga ggctgttcct
     3841 gatcctggtg ctgctggtgc tcgccggcgg gtatctcggc tggccctact acaccctgta
     3901 ccggatcgac gtcgccctgg ccggaaacga caccgccacg ctcgatcggc tggtcgactg
     3961 gggctcggtg cgcgcaggcg tggagcggga gctcgccgcc cgcaacgtgt cgcgcgagga
     4021 caccggtgcg ggcgcgagcg gcgaacggcc cggcggcctg gccggattgg tcgtgcccag
     4081 gttcggccag atgctggtcg acgcttacgc cagcccgcgg ggcctggccc ggctgatgcg
     4141 cgatgcccgg gtcgcgccgc cggcggcgcg ggcgagcgcg cgcgccggcg ccgggaccgg
     4201 cgcgccggcg ccgttccgcc cggcgctgtt cgaggcgatc tcctatgcca gggacaaggt
     4261 cgcggcggcc gaggcgcttg cctgcgcgac ctaccggcgg ctgcgcgacg actacgacta
     4321 tctgttcttc accaacccgt tcacgttcga ggcccgctac gcgccgcgcg ccgccgatca
     4381 gaccgagccg ctgatcctga cactgcggtt ccaggacctg atctggcgcc tggaccgggt
     4441 cgacctgccg agcgctcgcg ccggcgacgg cggttagccg agcggcggga agcttgcgcc
     4501 gcgggccgca atggccccgg ggcctcggtg cgacaacgcg gtcactcgcg caccgaggtg
     4561 agcctgagca gctcgcgctc ggccgccgcg tactgggcga atgcggtgac gccggtgtcg
     4621 agagttgacc ggaagtagtc cttagcgtgc gaaggatcgc cgcgcagcag cgcccgctgg
     4681 ccgagaaaga aataggcgac gcacagccgc tcgcggcggg tgatcgggtc cttggactcc
     4741 gcttcggcca gcacggcgtc ggtgctcgac tcgcccagat agtagcgggc cagcacgccc
     4801 ggccaacgat ccaggtcgac cgcgcgcgcg tccgccgcca actgggcggg cgcgtcctgc
     4861 ccgtcgcgct cgaaggcgat gtagcgccac agcatcgcgt agcgcttgag ctcgccgtca
     4921 ccgaggtcga aggcagtggc gaaatcgttc cccgcctcgg cgtggcggcc gcggtacaga
     4981 agcgcgatgc cgcggctgtt gtaggcggtg gcgaagtcgg gcttgagccg caccgcctgg
     5041 ccatagtcgg cgatcgcctg gtcgagccgg tcgagccgct cattcgccca ggcgcgcccg
     5101 aaataggcct cggcgtagtc cgggcgcagc gccagcgccc gggtgaaggc gtcgatcgcc
     5161 gagtcgtagc ggtcgtcgcg ctcgcggtcc cagccgagct cgttcagctg cgcggacttg
     5221 gctatcgcct cgcgatcggt ggtcgggcgc ggcaggggct cctggccggc cggcgcgggc
     5281 agcgcggcga ccacggccgg cgcctgcgcg accaccggcg ggggtggcgt gcccgcggcg
     5341 acgttcgggg gcggcggcac cagctcggcg agcggcagcg acggcaccga caacggcccc
     5401 actccctgcg gaacctgctg gtcgggaatc gccggcgacg cttgcggctc ggccggcggc
     5461 ggcgccggag cgggcgcgag ccttgccgcc gcgagcgtgg tgggcgaatc ggcggacggt
     5521 cgaaccgggt cgacggtcgc caccagcaac gccggcattg ttgcaggctc accgactgcc
     5581 tcggcgacgg ccaccacctc gcgcccgccg tcgggtgcct cgccgcgccc gaggacgagg
     5641 acggcgccgg ccgccgcgac cgccgccgcg cccgcgccgg cggcgagcca cggccatcgc
     5701 cgccgcgccc gcgccggctt gagcccgccg gcggccgcca ggcgcaccgg caacggcggt
     5761 ctgccggtcg cctggcgttc ctgttccgat ttcctgagcg cgttcagtat gtaggacatg
     5821 ggcgaccggt cccgaccgat tgaggctctg gatccggcac tagcgcagcg acggcgcccg
     5881 gtagccgggc atcgtctggc tgaggcggat catggtgcgc ggtccggcga cgccgtcgac
     5941 ctccagctcg tggtcgcgct ggaacatgcg cacttgcgcc gccagggagt cgtcgaacac
     6001 cgcgcgggcc gcctttgccg gcggggcgcc gcgcagctcg gcaagccggt tttgcagcca
     6061 gacgacgtcc tcgcccttga cgcccggctt gagctggcgc tcgaagttgt acggcgggcg
     6121 ccaaagcatc aggtagtcgc cggtccacag ggccgacaac gtggcgacgg tggtctcgac
     6181 ctgctcgccg ccgacgatca ccgtcgcgga gccgtcgccg aggccggtca gcgccaggtg
     6241 gacgcggcgc ccgtcggcca ggtgcagggt catcaccacc ggcaggtcgg cgatgcgcac
     6301 ctggtgccag tcggtgctgc cctcctggca caacagattg acggctcgcg ccttccggca
     6361 cgccgtcatg cccgtgagct cttcgtagtc gaatccccac accgcgaaca ggtcggcgac
     6421 cgccgtttcg aagtcgccgg tggaccccgg gccggacagg cggtcttcga gcgacaccgg
     6481 cgccgggcgg gtagccgaag gcggcgtcac cgcggccatc accacggcct cgtccgccgt
     6541 cgcgcccgcc ggcccggacg gccacggcgg cggcagcatc gcgaccgcga tcatggcggc
     6601 cggcggctcg gaaatgggcg gtgccggcag ccgcgcgacc gtgaacccgg cggccggcga
     6661 cggcggcacc tcggctggcg gtgtggcggc ggcgcccggg tcgctgccga caccgggcaa
     6721 ggccgccgtc gtcggcccgg cggcggtgac gaacacgtcg tcgagcaccg gctccgccgc
     6781 cgcggtggtc gcagcggcct cactcaccgg ccccggcccc ggacccggat cgagaccggg
     6841 ccacgagcgc aggccgtacc ggtccgccgc gaccaccgcg ccggcgatcg cgaccgtggc
     6901 cgcggcggcg gcgaaccgcc accggccgcg gcccgcctgc gggtcgcgcc cgcgcacttc
     6961 cgacgccgcc ccgcgcacca gccgccggtc gacgacgctt cggtcctggc cgtaggcgcc
     7021 gagcagcgcg cggtcgcaca gggtgttgat caggcgcggc acgccacccg accggcgatg
     7081 gacctcgcgg atcgcccgcg gggtgaagat ctcgcgcgtc gcgcccgcaa cctcgaggcg
     7141 gtggcggaca tagcccgcgg tctccgccgc gctcaacggc tcaaggtggt agcgcgcggt
     7201 aatgcgctgg tcgagctggc gcaggcccgg gcgcctgagc acgtccctga gctcgggctg
     7261 gccgatcagg acgatcttga gcagcttgcg cgtggtggtc tcgaagttgg tcagcagacg
     7321 gacctgctcg agcacgccgg ggctcaggtt ctgggcttcg tcgatcagca gcaccggctg
     7381 gcggccgcgc gcgttggccg cgagcagatg ctggttcagg cgatcgacca aaagcttcag
     7441 cgacggattc tcgcgcgcgt atggcacgcc catctcctcg cagatggttg ccagcagctc
     7501 gatctcgttg acccgggggt tgaggacgaa ggcgaggtcg acgccgtccg gcagctgctc
     7561 gatcaggctg cggcacagcg tggtcttgcc ggtgccgacc tcgccggtga gcagcacgaa
     7621 cccgccgcct tcgccgatgc cgtagaccag gtgggccagg gcctcctggt ggcgcttgct
     7681 catgtagagg tagcgggggt ccggcgctat cgagaacggg ttctcgtcga agcccaaata
     7741 ttcccaaaac atgctttcgc cttgtcgcgg cgtggcgcag gccgccggca ccgggatgcg
     7801 cgcctcgacc gcgccgccat ccgctgctcg cgccgatcgc tcggcacatc ggtatcggcc
     7861 gcgtccccgg ccggccgtcc ccctgcaaat gcaggccgag agtctaccat gcgcgggacc
     7921 gcttgcaacg tcgccggccc gccgcgccgg cgtcggtcag ggttcgacca ccacccggtc
     7981 gcgtccgctc tcctttgccg acatcatcgc cgtcgtcgcg cgcctgagca gctcgctgcc
     8041 cgactcgccg ttgcggtatt gggtcacgcc gaccgagagc gtgatggtgc cgtagcgctc
     8101 gccggtcttg cgattggtca gcgtcttgcg gccgaacgcc gcgcggacgt tttccgcaac
     8161 cttgaccgcg ccgtccttcg cggtctccgg caacatgatc gcaaactcct cgccgccgat
     8221 ccgcccgatc gcatcctggc ccttgacgca ctcgacgatg gtgcgcgcga ccagacgcaa
     8281 cacctggtcg ccgaccgtgc gcccgtaaga ccggttgaac tgttcgaaat ggtcgagatc
     8341 gagcacgcag acactgagct ggccttggtc ctcgcgcgcc cgatcgacgg cgatcttcag
     8401 ggcgagatgg aagaacttga ggttgcgcac gccggtcagg gcgtcggtca gcgtctcctc
     8461 gcgcacgccc cggaagtcgc gctcgatctc cttgatctcg aacacggtat tctcgagctt
     8521 gtcggccagc ttgcgggcct cgtcggtgag cgagcggctg tcggtcagca cccggtcgac
     8581 cacggcgccg atctcgcccg ccacttcgcc gaggttgctg gcaagctcgc cggtctcgct
     8641 gtcgatggaa tgcgcctcgg cgaccaggtt gtcgatcatc gacttgaccc cgacggcggc
     8701 ctcattgccg gacagttggg cggagtattg ctgcagccgg tcgctgatct cgttggtgcg
     8761 ctggttcgcc gccgccgcat attgctgggc gcgacgaagc tcgccgacga ttcgctcgct
     8821 ggtgtcctcg accagggcgg actcccgcgc catgctgaag tacttatcgt agatctcggc
     8881 gcacaggtcc tcggtgaacg ggcgccgctc ggcgatcgcc tcgtcgatgg tacggcacag
     8941 gtccggatac ctgcccgccg cataggcgta ccagaccgcg atattgctgg ggttggtcgg
     9001 cacattgtgc tgagccatga acgagagtcc ggcgaagacc tcttccgcga cctcgtccgg
     9061 atgatccgtg tagtgcaccg gtcgaatctc ccgccgtcct acgagtcgtg gaccggccgc
     9121 gtcggccgcc gcgccgccgt ccgtgcggtg gccgggggca acgcccgatc tgtcccaagc
     9181 taagtatata tgggccccgg cgcgccttac ccaccgccgg ggacaaaaaa acggcgcccg
     9241 gctagcgctc gcgctggatc cagatcaaga ggccggtaag caccagcacg atgacgattc
     9301 cgagcgggaa caggatcagg gtgagcatgg ggtccatgcg gaacatgacc tggatggtca
     9361 cgtagacgct gcgcgcccat tcgctgacca cgttgtcgat tcggtgatcg tgggcgaact
     9421 ggaacagcgc ccagccgatc aggacgcagg ccgccaagac cgccaagccc agaaccctgc
     9481 cggcgattct ggcgaaagtc tgcatgctgg cctcgcctcc cgctgatcgg gtgccgggct
     9541 tgccacccgc ccgcaacacc agcttggtgg cgcatcgcgt cgggagcggc gtccgtgccg
     9601 acagccaatg ttttgcgccg gcgcggtcct gtcaagccgc tcgcgccgtg cccggcggcg
     9661 ctccgcgggc cgcgctcggc acggcgcggc ggcgggcggg ggttggagcg cgcggatgga
     9721 attctatcct tgaatgatgt taggctttga tcgagcgcag gcatcgggcg gcgcgggccg
     9781 gccctcggct caggagggct ccgtggggcg tcttgcgcaa gtggtgtacg cgcatgggcc
     9841 gtgagcgagg ggcgctggcc gtgatcgcgc gcgaatcgag tggggcaagg cgcggcgttc
     9901 gtgcgccgct ctgcgtggtc atttgggtgc tggtgctgtc ggcgctgggt ttggcgccgc
     9961 cggcggcatc ggcgccactg cactccgaag gcgcggcttc gccggccctg cccggactcg
    10021 gtcctgacgg tcttcccggc cgcctcgggg ctttgcagcg acttgccgat cgcgacttcg
    10081 acggcatcgc cgactcgctg gccgagcggc tccagaggct cgcgccggcc gaccaggtcg
    10141 acgtgatcgt caccttcgcc gcccggcgca ccgccgccga cgcgcgcgcg caaatcgggc
    10201 cgttcacgct caagcgcgag tttcgaatca tcaacggctt cgccgcgcgc atgccggggg
    10261 ccctggtgcg gaggctcgcc agcatgccgg gcatcgcccg catcgaggag gacttcgagg
    10321 tccacgcggt cctcgacatc gtcacccggg acaccggcgc gagcgacgcg cgcgccgact
    10381 tcgccgtcac cggtttcggc accaccggct gcgtcgtcga taccggcgtc gatcccaacc
    10441 acgagcagct cgacagcaag accatcggcc cggcggattt cgccgacttc atcaacagcc
    10501 gcaccaatcc ctacgacgac cattggcacg gcacccacgt cgccgccatc atcgccggcg
    10561 acggcaccgg cacatcggcc tctgcgcaac ggttccgggg cttcgcgccc gatgccgaca
    10621 tcgtcgccgc caaagtgctc aactccaacg gcagcggcct ggcgagccag atcatcgccg
    10681 gcatcgagtg gtgcgcgtcc cgggcggacg tcgatgtcat caatttgagc ctcggcggcg
    10741 ggccgaccga cggcctcgat gcgatgagcc gggcggtcaa ctgcgcgacc gacccgacct
    10801 ggagcacgag ctgcggccaa gtgaacggcc acccgaaggt ggtgctggtc gcggccggca
    10861 actcgggcgc cgcaccgtcc acggtcagca ccccgggtac cgcggaactc gcgatcacgg
    10921 tcggcgcctt cgccgactgg tcggggtcgc ccggcagcgg cggccaggac gacggcctgt
    10981 acctgcgcag cttctcgggc cgcggcccgg tgaagaccgg ggccggcgcc gtgcgttacg
    11041 tcaagcccga tctcgccggt cccggctcgc gggtcggctc cgcctattcg gcgaacggac
    11101 ccaccggtta cgccttcgcc agcggcacct cgatggcgac gccgtcgatc gcgggcatcg
    11161 tcgcgctgat gatcgaggcc aacgacgtct actccgacgg tctgctcatg accgccgatg
    11221 cgaacggcct gccggcggac aaggtgcgcc agatctttga actgacgtcc acggacctgg
    11281 gcccgccggg cctcgacaac gagtacggct acggcgtggt cgacgcctac gccgccatcg
    11341 ccgaggccgc gggccaatcc tacgacccga cgccgctgcc ccagcacctc cgcctggcgg
    11401 cccagtcggt cgccaacggc ggcagctgga cctacgggtt ctcgatcggt gccga
//
LOCUS       DASWHU010000359         7173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3548788, whole genome shotgun sequence.
ACCESSION   DASWHU010000359 DASWHU010000000
VERSION     DASWHU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548788"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGB88_13365"
     CDS             complement(<1..947)
                     /locus_tag="VGB88_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9648487.1"
                     /translation="MSDGAERPLPYGRHAVDDDDVAAVARTLRGDWLTGGPAVAGFEA
                     ALTRVTGASHAIACASGTAALHLAALALDLRPGDAVVVPTLTFLATANAPRHAGAEIA
                     FADVDPDTGLMGVAELEAALARAEAEGTGRVRAAFPVHLNGQCADPAGLAAACRARGL
                     AVVADAAHAFATRYAAGGAERAVGADADALLTAFSFHPVKVIAMGEGGAVTTGSPALA
                     ERLTRLRNHGIARDAGAFRNRDLAFDSGGAANPWYYEMAEPGLNYRASDIHCALALSQ
                     LAKLDRFVARRAALVARYDALLAPLAPAVRPVARAPGCRP"
     gene            complement(949..2670)
                     /locus_tag="VGB88_13370"
     CDS             complement(949..2670)
                     /locus_tag="VGB88_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX9648488.1"
                     /translation="MTASAIGGLLARLETVVDVAARRPGHLAWPSRARLADRLRALFY
                     ATPFYSLTLVGRVPERMAVQPPDPWPGSAVRGDALFQGRYRFAGHEVSAPKRPVWLPV
                     GANAAWLEELHAFEWLRDFKASGGNSARRHARALIGDWLGQFGHWRPLVWRADVQGRR
                     IAAWAGAAGFLLNGAEPGWRRGFLASLSMQLRHLARTAQGDVDGARRIAALRGLLYGG
                     LCLGGGEARVALAVRGLVRECRRQVLADGGHLERSPRLQRRVLGDLADCRALLRAANR
                     VPPEELTRTIDRMAPMLRALCHGDGGLAQFNNSGEGDAALVAATLALAEASGSPQANA
                     PHVGFQRLAAGGTVVIVDCGAPYPGSDHAHAGTLAVEMSAGSHRLVVNCGPHSGREPQ
                     WVQALCSTAAHSTLVVADTSSSAVRGDGSLGRRPLKVACRREQADGAIWLDASHDGYA
                     ANFGLIHRRRLYLESAGGDLRGEDSLFPSGEPGPPGPQPFAVRFHLHPSVQASLVLHG
                     QAALFRLADGSGWQLRAHGGSLALNASVYIDADGERRRCEQAVIAGVTGPEGTTVKWA
                     ITRVAGS"
     gene            complement(2681..3352)
                     /gene="rpe"
                     /locus_tag="VGB88_13375"
     CDS             complement(2681..3352)
                     /gene="rpe"
                     /locus_tag="VGB88_13375"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9648489.1"
                     /translation="MQQRVRIAPSILASDFARLGEELRALTEAGADYIHIDVMDGHFV
                     PNITVGPGVVQALRAHSDRPFDVHLMIAPVDPYLDAFADAGADILTVQAEAGPHLHRS
                     LQRIRSLGKRAGVAINPATPASVIEPVLGDVELVLVMTVNPGFGGQTFIDGQLDKIAR
                     VRALIDGADRAIDLEVDGGINFKTAARAVAAGADVLVAGTAAFVGGPDAYGVNIARLR
                     GCAPA"
     gene            complement(3415..4728)
                     /locus_tag="VGB88_13380"
     CDS             complement(3415..4728)
                     /locus_tag="VGB88_13380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX9648490.1"
                     /translation="MRYTGAMDDDLAARALALDWLEMVLARRRTLDDAIAELTGAALA
                     ERDRAFARALAACVLRRLGQIDALIGRCLESPRAAGARAARALLRLGAAQLLFLDTPP
                     HAAVDTTVRLARARGLDALTGLINGVLRRLARDGAAWREAQDAARLNTPDWMWRSWTA
                     AYGERPARAIAAAHLDEPPLDLTPKRGAQAAALARRLDAKVLATGGLRLAARARIESL
                     AGYGEGEWWVQDAAAALPARLLAPVAGRRVIELCAAPGGKTAQFAAAGADVVAVDRSS
                     PRLELLAANLRRLGLTAETVCADAGRWRPQRPAELVLLDAPCTGTGTIRRHPDILRLR
                     RAEDVARQAALQRRLLDNAAAMLAPGGLLVYAVCSLEPEEGPELVEGLLAGGAPFERV
                     RIEAGEVGGLTELVSPAGDLRTLPCHLADQGGLDGFYAARLKRLA"
     gene            complement(4763..5614)
                     /gene="htpX"
                     /locus_tag="VGB88_13385"
     CDS             complement(4763..5614)
                     /gene="htpX"
                     /locus_tag="VGB88_13385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX9648491.1"
                     /translation="MNLIRTAVLLAAITALFGGVGYLVGGQTGMAVALALALAMNAFA
                     WWNSDRMVLRLYKAREVDERSAPELYGIVRQLAERARLPMPKVYLIDSDQPNAFATGR
                     SPSRAALAATTGLLARLTPQEIAGVMAHELAHVRNRDTLTMTVTATLAGAVSMLANFA
                     LFFGNNRNNPLGLVGVLLVMIMAPLAAALVQMAISRTREYAADRGGAEICGHPLWLAS
                     ALDKISSLARRIDNTGAERNPATAHMFIVNPLHAHRRDGLFSTHPNVENRIRALREMA
                     GVAGPWG"
     gene            5680..5877
                     /locus_tag="VGB88_13390"
     CDS             5680..5877
                     /locus_tag="VGB88_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEX9648492.1"
                     /translation="MTDQRAPAPEASDRSDPAPEPAVGAPRPPRPGRVPAQAPGEVGG
                     PDGPEPTRYGDWERKGRVSDF"
     gene            complement(5890..6150)
                     /locus_tag="VGB88_13395"
     CDS             complement(5890..6150)
                     /locus_tag="VGB88_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648493.1"
                     /translation="MARRFVTVWAGAGLLCLGASAVQAMPLEHDWLSTPGVTQLGTLD
                     IKEVMDNSTEMEMRLSKQIMVLQQQILDLRRELDAVRAAQQR"
     gene            6408..6890
                     /locus_tag="VGB88_13400"
     CDS             6408..6890
                     /locus_tag="VGB88_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9648494.1"
                     /translation="MPSARGWPPVLFVVALIALTGFDAPETGGAETLPVPVRAEVVSV
                     IDGDTLLVRARIWLDVEVEVHVRVAGIDAPERHGKCTEERALAERARDLVATTVDGAE
                     VMLSELQHDKYAGRVLARVAIPDGRDLGALLLAAGVARAYDGGPRQDWCREAAEVREN
                     "
     gene            6945..>7173
                     /locus_tag="VGB88_13405"
     CDS             6945..>7173
                     /locus_tag="VGB88_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648495.1"
                     /translation="MVASKCWHGREGTRAGSGPIRWAVAACLVAALAGCIRAEAAALP
                     ADGPDARLRALAEAGDAGAMTALGARSEHGAG"
BASE COUNT          943 a         2583 c         2762 g          885 t
ORIGIN      
        1 gggcggcagc cgggggcgcg cgcgaccggc ctgaccgccg gggcgagcgg ggcgagcaag
       61 gcgtcgtagc gggcgaccag cgccgcgcgg cgggcgacga agcggtcgag cttggcgagc
      121 tggctcaggg cgagcgcgca gtggatgtcg ctggcgcggt agttgagccc cggctcggcc
      181 atctcgtagt accaggggtt ggcggcgccg cccgaatcga aggcgaggtc gcggttgcgg
      241 aacgcgcccg cgtcgcgcgc gatgccgtgg ttgcgcagcc gcgtgaggcg ctcggcgagc
      301 gcgggggagc cggtggtcac cgccccgccc tcgcccatcg cgatcacctt gaccgggtgg
      361 aacgagaacg ccgtgagcag agcgtcggcg tcggcgccga ccgcgcgctc ggccccgccc
      421 gcggcgtagc gggtggcgaa ggcgtgggcg gcgtcggcga ccacggcgag cccgcgcgca
      481 cggcaggcgg cggcgaggcc ggccgggtcg gcgcactggc cgttgaggtg caccgggaac
      541 gccgcgcgca cccgccccgt cccctcggct tctgcacgcg cgagcgccgc ctcgagctcg
      601 gcaacgccca tcaggccggt gtcggggtcg acgtcggcaa aggcgatctc ggcgccggcg
      661 tggcgcggcg cgttggcggt agcgaggaag gtgagcgtcg gcaccaccac cgcatcgccc
      721 ggtcggaggt cgagggcgag cgcggccaag tgcagcgccg cggtgccgct cgcgcaggcg
      781 atggcgtggg aggcgccggt gacgcgggtg agtgccgcct cgaagccggc caccgcaggc
      841 ccgccggtca gccagtcgcc gcggagcgtg cgcgccaccg cggcgacgtc gtcgtcgtcg
      901 accgcgtggc ggccgtacgg cagcggccgc tcggcgccgt cgctcacgct agcttccggc
      961 gacccgggtg atcgcccatt tgacggtggt gccctcgggg ccggtgacgc cggcgatcac
     1021 cgcctgctcg cagcgccggc gctcgccatc ggcgtcgatg tagacgctcg cgttcaacgc
     1081 cagcgagccg ccatgcgcgc gcagctgcca gcccgagccg tcggcaaggc ggaacagcgc
     1141 cgcctggccg tgcagcacca gcgacgcctg cacgctcggg tgcaggtgga agcgcaccgc
     1201 gaacggctgc ggcccgggcg ggcccggctc gcccgagggg aacaggctgt cctcgccgcg
     1261 cagatcgccg ccggcggact cgagatagag gcggcggcgg tggatcaggc cgaagttggc
     1321 ggcgtagccg tcgtggctcg catccagcca gatggcgccg tcggcctgct cgcgccggca
     1381 ggccaccttg agcggccggc ggccgaggct gccgtcgccg cgcaccgcgc tcgagctggt
     1441 gtcggcgacc accagggtcg aatgcgcggc agtgctgcac agcgcctgca cccattgggg
     1501 ctcgcgtccc gaatgggggc cgcagttgac caccagccgg tgcgagcccg cgctcatctc
     1561 gacggcgagg gtgccggcat gggcatgatc cgagcccggg tagggcgcgc cgcagtcgac
     1621 gatcaccacc gtgccgccgg cggcgagccg ctggaagccg acatggggcg cgttggcctg
     1681 ggggctgccg ctcgcctcgg cgagggcgag ggtagcggcg accagcgctg catcgccctc
     1741 gcccgagttg ttgaactgcg ccaggccgcc gtcgccgtgg cacagcgcgc gcagcatcgg
     1801 cgccatgcgg tcgatggtcc gggtcagctc ctcgggcggc acccggttgg cggcgcgcag
     1861 cagcgcgcgg cagtcggcga ggtcgccgag cacgcgccgc tgcagccgcg gcgagcgctc
     1921 gaggtggccg ccgtcggcga gcacctggcg gcggcattcg cgcaccagcc cgcgcaccgc
     1981 gagcgcgacc cgcgcttcgc ccccgcccag gcacaggccg ccatagagca gcccgcgcag
     2041 cgccgcgatc cggcgcgcgc cgtcaacgtc gccctgcgcg gtgcgcgcca ggtggcggag
     2101 ctgcatcgac aggctggcga ggaagccgcg ccgccagccg ggctcggcgc cgttgagcag
     2161 gaagccggcg gcgcccgccc aggcggcgat ccgccggccc tgcacgtcgg cgcgccagac
     2221 cagcggccgc cagtggccga actggccgag ccagtcgccg atcagggcgc gggcgtggcg
     2281 gcgggcggag ttgccgccgc tggccttgaa gtcgcgcagc cattcgaacg cgtgcagctc
     2341 ctcgagccac gccgcattgg cgccgaccgg cagccacacc ggccgcttgg gcgcgctcac
     2401 ctcgtggccg gcgaagcggt agcggccctg gaacagcgca tccccgcgca ccgcgctgcc
     2461 gggccaggga tccggcggct gcaccgccat ccgctcgggc acccgcccga ccagggtcag
     2521 gctgtagaac ggggtggcgt agaacagcgc ccgcaagcgg tcggcgaggc gcgcgcgcga
     2581 cggccacgcg agatgccccg gccgccgggc ggcgacgtcg accacggtct ccagccgcgc
     2641 cagcaggccg ccgatcgcgg atgcggtcac ggccgggcgc tcaggccggc gcgcatccgc
     2701 gcagcctcgc gatgttgacg ccataggcgt cgggcccgcc gacgaaggcg gcggtgccgg
     2761 cgaccagcac gtcggcgccg gcggcgacgg cgcgcgccgc ggtcttgaag ttgatgccgc
     2821 cgtcgacctc gagatcgatc gcgcggtcgg cgccgtcgat cagcgcgcgc acgcgcgcga
     2881 tcttgtcgag ctggccgtcg atgaacgtct ggccgccgaa gcccgggttg acggtcatca
     2941 ccagcaccag ctcgacgtcg cccagcaccg gctcgatcac gctcgccggc gtcgccgggt
     3001 tgatcgccac gccggcacgc ttgccgagcg aacgaatgcg ctgcaagctt cgatgcagat
     3061 gcggcccggc ctcggcctgg acggtgagga tgtcggcgcc ggcgtcggcg aaggcatcga
     3121 gatagggatc gaccggcgcg atcatcaggt ggacgtcgaa cggccggtcg ctgtgcgcgc
     3181 gcagcgcctg cacgacaccg gggccgacgg tgatgttggg cacgaaatgc ccgtccatca
     3241 cgtcgatgtg gatgtagtcc gcgccggcct cggtcagcgc ccgcagctcc tcgccgaggc
     3301 gagcgaaatc ggaggcgagg atcgacggcg caatgcgcac gcgctgctgc atggacgttg
     3361 acctaccagc ttattccggg cggcgcaaat attgcgcggc agtgccggtc ggtatcaggc
     3421 taaccgcttc aggcgcgcgg cgtagaagcc gtcgagcccg ccctggtccg cgaggtggca
     3481 gggcagcgtg cgcagatcgc cggccgggct gaccagctcg gtgaggccgc cgacctcgcc
     3541 cgcctcgatg cgcacgcgct caaagggcgc gccgccggcg agcagcccct cgaccagctc
     3601 aggcccctcc tcgggctcga gcgagcagac cgcgtagacc agcagcccgc cgggggcgag
     3661 catggcggcg gcattgtcaa gcaggcggcg ctgcagcgcg gcctggcgcg cgacgtcctc
     3721 ggcgcgcctg aggcgcagga tgtccggatg gcggcggatg gtgccggtgc cggtgcacgg
     3781 cgcatcgagc agcaccagct ccgccggccg ctgcgggcgc cagcggccgg catcggcgca
     3841 cactgtctcg gcagtgagac cgagccggcg caggttcgcc gccagcagct cgaggcgcgg
     3901 gctagagcgg tcgaccgcga ccacatcggc gccggcggcg gcgaattggg cggtcttgcc
     3961 gcccggcgcc gcgcacagct cgatcacccg ccggcccgcg accggggcga gcaggcgcgc
     4021 cggcagcgcc gccgccgcat cctgcaccca ccactcgccc tcgccgtagc cggcgagcga
     4081 ctcgatgcgc gcgcgcgcgg cgaggcgcag gccgccggtc gcgagcactt tggcgtcgag
     4141 gcggcgggcg agcgccgcgg cctgcgcgcc ccgcttgggc gtcagatcga gcggcggctc
     4201 gtcgagatgg gcggccgcga tcgcgcgcgc cggccgctcg ccgtaggccg cggtccacga
     4261 ccgccacatc cagtcggggg tgttcagccg cgccgcgtcc tgggcctcgc gccaggcggc
     4321 gccgtcgcgc gcgagccgtc ggagcacgcc gttgatcagg ccggtgagcg catcgagccc
     4381 tcgcgcccgc gccaggcgca cggtggtgtc gaccgcggca tggggcgggg tgtcgaggaa
     4441 caggagctgc gccgcgccga gcctcaatag cgcccgcgcc gcgcgcgccc cggccgcgcg
     4501 cgggctctcg agacagcgcc cgatcagggc gtcgatctgg ccgagccggc gcagcacgca
     4561 ggccgcgagc gcgcgcgcaa acgcgcggtc gcgctccgcc aacgccgcgc cggtcagctc
     4621 ggcgatggcg tcgtcgagcg tccgcctgcg cgccagcacc atctccagcc agtcgagcgc
     4681 gagcgcgcgc gccgcgaggt cgtcgtccat cgcgccggtg tagcgcaccg gacgcgcggg
     4741 tcaatgcgtc ggaatcaagg gctcagcccc atgggccggc gacgccggcc atctcgcgca
     4801 gcgcgcggat gcggttctcg acgttgggat gggtcgagaa caggccgtcg cggcggtggg
     4861 cgtgcagcgg gttgacgatg aacatgtgcg cggtcgccgg gttgcgctcg gcgccggtgt
     4921 tgtcgatgcg gcgggccaag gacgagatct tgtcgagcgc gctcgccagc cacagcgggt
     4981 ggccgcagat ctcggcgccg ccgcggtcgg cggcatactc gcgcgtgcgg ctgatcgcca
     5041 tctgtaccag cgccgccgcc agcggcgcca tgatcatgac cagcagcacc ccgaccaggc
     5101 cgagcggatt gttgcggttg ttgccgaaga acagcgcgaa gttggccagc atcgacaccg
     5161 cgccggcaag cgtcgcggtc acggtcatgg tcaaggtgtc gcggttgcgc acatgggcga
     5221 gctcgtgcgc catcacgccg gcgatctcct ggggcgtgag gcgcgcgagc aggccggtgg
     5281 tcgcggccag cgccgcgcgc gacgggctgc ggccggtggc gaacgcgttc ggctggtcgc
     5341 tgtcgatcag atagaccttg ggcatcggca ggcgcgcgcg ctcggcgagc tgacgcacga
     5401 tgccgtacag ctcgggcgcg ctgcgctcgt cgacctcgcg cgccttgtac aggcgcagca
     5461 ccatgcggtc cgagttccac caggcgaagg cgttcatcgc cagcgccagc gccaacgcca
     5521 ccgccatccc ggtctggccg ccgaccagat agccgacgcc gccgaacagc gccgtgatcg
     5581 cggccagcag gaccgccgtg cggatcaggt tcatggggcg atatttgggc ggcggcccga
     5641 cgccttcaag agcggcccgc ggcgcgctat cctgggcgca tgaccgacca gcgtgccccc
     5701 gcccctgagg caagcgaccg ctcggacccg gcgccggagc ccgccgtcgg cgcgcccagg
     5761 ccgccgcgcc ccgggcgcgt ccccgcccag gcgccgggcg aggtcggcgg ccccgacggc
     5821 cccgagccga cccgctacgg cgactgggag cgcaagggcc gggtcagcga cttctgagcg
     5881 tggctgcggc tagcgctgtt gcgccgcgcg caccgcgtcg agctcgcgcc tgaggtcgag
     5941 gatctgctgc tgcagcacca tgatctgctt ggacagccgc atctccatct cggtcgagtt
     6001 gtccatgacc tccttgatgt cgagcgtgcc cagctgggtc accccggggg tgctcagcca
     6061 gtcatgctcg agcggcatcg cctgaacggc ggatgcgcca aggcacagca gcccggcgcc
     6121 cgcccagacc gtcacgaacc ttctcgccat gcgtagcctc ctgtggctct ggtcatcgcg
     6181 tcatcgacgc gccaccgccg gcccgagtcg atgcgggccg cacggttcgc ggaagcgtga
     6241 cgcggtcggg ccgcgattgg caataccgat ctgatcgcgg gcgcgcgtgc cgtcgccggc
     6301 aaaatcggct atgttccatt tttgttctgg catattgacc ctaattgcgc tagttctatt
     6361 tgaggactac ccgcattacg tgtggttatg ggattgtctt ggtctcgatg ccgtcagcga
     6421 ggggatggcc gccggtcctg ttcgtcgtgg cgctgatcgc gctcaccggc ttcgacgcgc
     6481 cggaaacggg cggcgccgaa actctgcccg ttccggtgcg cgccgaggtg gtgtcggtga
     6541 tcgacggcga tacgctgctg gtgcgggcca gaatctggct cgacgtcgag gtcgaggtgc
     6601 atgtgcgcgt ggccgggatc gatgcccccg agcggcacgg gaaatgcacc gaggagcggg
     6661 cgctggcgga acgcgcgcgc gacctggtcg cgaccacggt cgacggcgcc gaggtgatgc
     6721 tcagcgagct ccagcacgac aaatatgccg ggcgggtcct ggcacgggtg gcgatcccgg
     6781 acggccgcga cttgggcgcg ctgctgcttg ccgccggcgt cgcccgcgcc tatgacggcg
     6841 ggccgcgcca ggactggtgc cgggaggcgg cggaggttag agaaaattga ccgctcggcg
     6901 tatatctggg gcgggttgcg cgacggcccc ggcagggttc gcggatggtg gcaagcaagt
     6961 gctggcacgg gcgcgagggg acgcgcgccg ggtccgggcc gatccggtgg gcggtcgcag
     7021 cctgcctcgt ggccgcgctc gccggctgca tccgggccga ggccgcggcg ctgccggcgg
     7081 acggacccga cgcccgcctg cgcgcgctgg ccgaggctgg cgatgccggg gcgatgaccg
     7141 cgctcggcgc gcgctccgag cacggcgccg gcc
//
LOCUS       DASWHU010000360         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_611205, whole genome shotgun sequence.
ACCESSION   DASWHU010000360 DASWHU010000000
VERSION     DASWHU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611205"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VGB88_13410"
     CDS             complement(<1..893)
                     /locus_tag="VGB88_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648496.1"
                     /translation="MTAAAPARARAPGAWTALRAAKVALPVAALVLVPLLADSPFVTR
                     VFVSALMFGILGAIYDLMLGYAGLINFGFGGFLAVGAYASALASVHYGISPWLGLGIG
                     GLATAFVGLFTGVITLRLKGLYLALMTFFVGEAIRSTIANTPETTRGVLGLAVEPFPD
                     LLGIGFGRDDPLAYYYLLLVLGALIMLAMVLLVRSRYGLAFKAIREDQLAAESLAIDA
                     TRYKLVNFTVASFLTGVVGAFYGHYLGILTPTPEEFGVPRTVEVLIVTYVGGRGTLWG
                     SLFAGFLLIGFQEYFRSLGAWR"
     gene            complement(890..1798)
                     /locus_tag="VGB88_13415"
     CDS             complement(890..1798)
                     /locus_tag="VGB88_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648497.1"
                     /translation="MDLALLLEPSVLAEVLVQGLVRGAMYALMAAGLAVIFGILGVAN
                     FAHGALFMLGSYAMVLVGIDLGLPFAAGIAAAVLVLFAVGFALERTVIETLRRRAGRD
                     WLLDSFVLTIGLMVVLENLALIAFGSRRRGISQLIDGSLELGRAIISYERLFILVAAA
                     AAGAALWAGMRYSAFGKALRATAQNPEAAQTLGIDIARIYSYSFGLGAALAGLAGALL
                     ISIYPAYPTVGYQPVVKGLAVVILGGLGHVPGAVIGGLVLGVVEAYSLLFMASGWQNV
                     VTAGLVVLVLILRPSGLFVAAGGDRP"
     gene            complement(1798..2511)
                     /locus_tag="VGB88_13420"
     CDS             complement(1798..2511)
                     /locus_tag="VGB88_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648498.1"
                     /translation="MTLTVDGLVAGYTREVPILRGVALNAESGRVTALIGPNGAGKST
                     LLKTIYGYLVPERGRILHDGASLAGLAPKDMLGRGIAYLLQGRGTFPSMTVHENLELG
                     AWVLRRDRSAVAGAFERVYARFPRLKEKRRLAAGVLSGGEQRMLEIARLTMTGPRTLL
                     LDEPSVGLMPALVDMVYDELHRLKDEARFTIVMVDQNVKKAIGIADYVYVLRLGENHR
                     QGPQADFRDRLDQIIREWI"
     gene            complement(2519..>3071)
                     /locus_tag="VGB88_13425"
     CDS             complement(2519..>3071)
                     /locus_tag="VGB88_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648499.1"
                     /translation="ASAGRVLLAGRDITNRKLSRVAALGVGRTFQVARAFREMTVLDN
                     MLVAAVPRGLTGAKARDRARALLALAKLDGLADAPAVEISGGQQKLLEFVRTVMGEPR
                     LVLLDEPFSGITPALVERLIAMVRTLNAEHGTTFLLISHEIPEVAALCESVTVLAGGG
                     VIARGTPAEIRADPAVIEAYLGR"
BASE COUNT          534 a         1095 c         1078 g          364 t
ORIGIN      
        1 cgccaggcgc cgaggctgcg gaaatactcc tggaagccga tcagcaggaa gcccgcgaac
       61 agcgagcccc acaaggtgcc gcgccccccc acataggtca cgatcagcac ctcgaccgtg
      121 cgcggcacgc cgaactcctc cggcgtcggc gtgaggatgc cgagatagtg gccgtagaat
      181 gcgccgacga cgccggtgag gaagctcgcg acggtgaagt tgaccagctt gtagcgggtg
      241 gcgtcgatcg ccaggctctc ggcggcgagc tggtcctcgc ggatcgcctt gaaggcgagg
      301 ccgtagcgcg agcgcaccag cagcaccatg gcgagcatga tcagggcgcc taggaccaac
      361 agcaagtaat agtaggccaa cggatcgtcg cgaccgaagc cgatgccgag caggtcgggg
      421 aacggctcga ccgcgagccc gagaacgccg cgcgtggtct cgggcgtgtt ggcgatggtg
      481 gaccggatcg cctcgccgac gaagaaggtc atcagggcga gatagaggcc cttgagccgg
      541 agcgtgatga cgccggtgaa cagcccgacg aatgccgtcg ccagcccgcc gatgccgagc
      601 ccgagccacg ggctgatccc gtaatggacc gaggcgagcg cggaggcgta ggcgccgacc
      661 gcgaggaagc cgccgaagcc gaagttgatc agcccggcat agcccagcat caggtcgtag
      721 atcgcgccca gaatcccgaa catcagcgcc gagacgaaca ccctggtgac gaacgggctg
      781 tcggcgagca ggggcaccag gaccagcgcc gcgaccggca gcgcgacctt ggccgcgcgc
      841 agcgccgtcc aggcaccagg cgcgcgcgcc cgcgcgggcg ccgccgcggt cacgggcggt
      901 cgcctccggc ggcgacgaac agccccgacg ggcgcaggat gagcaccagc accaccaagc
      961 ctgcggtcac cacgttctgc cagccggagg ccatgaacag cagcgagtag gcctcgacca
     1021 cgcccagcac caggccgccg atgaccgcgc ccgggacgtg gccgaggccg cccaggatga
     1081 ccaccgccag ccccttgacc accggctggt agccgaccgt cggataagcc gggtagatcg
     1141 agatcaggag cgcgccggcg agccccgcca aggccgcgcc gaggccgaag ctgtagctat
     1201 agattcgggc gatgtcgatg ccgagggtct gcgccgcctc ggggttctgg gcggtcgcgc
     1261 gcagcgcctt gccgaacgcg gagtagcgca tccccgccca cagcgcagcg ccggccgccg
     1321 ccgcggcgac gaggatgaac aggcgctcat aggagatgat cgcccggccg agctcgagcg
     1381 agccgtcgat cagctggctg atgccgcgcc ggcggctgcc gaaggcgatc agcgccaggt
     1441 tctccagcac caccatcagc ccgatggtca gcacgaatga atcgagcagc cagtcgcgcc
     1501 cggcgcgccg gcgcaaggtc tcgatcacgg tgcgctcgag ggcgaagccg acggcgaaca
     1561 gcaccagcac cgcggcggcg atgccggccg cgaacggcag gccgaggtcg atgccgacca
     1621 gcaccatggc gtagctgccg agcatgaaca gcgcgccgtg ggcgaagttg gcgacgccga
     1681 ggatgccgaa gatgaccgca agcccggccg ccatcagcgc gtacatcgcg ccgcgcacca
     1741 ggccctgcac cagcacctcg gccagcaccg acggctcgag cagcagcgcg aggtccatca
     1801 gatccactcc cggatgatct ggtcgaggcg gtcccggaag tcggcttgcg ggccctggcg
     1861 atggttctcg cccagcctga gcacgtagac gtagtccgcg atcccgatcg ccttcttgac
     1921 gttctggtcg accatgacga tggtgaagcg ggcctcgtcc ttgagccggt ggagctcgtc
     1981 gtagaccatg tcgacgagtg ccggcatcag gcccaccgac ggctcgtcga gcagcagcgt
     2041 gcgcggcccg gtcatggtca ggcgcgcgat ctcgagcatg cgctgctcgc cgcccgacag
     2101 cacgccggcg gccagccggc gcttctcctt caggcgcggg aagcgcgcat agacgcgctc
     2161 gaacgcgccc gccaccgcgc tccggtcgcg gcgcaggacc caggcgccga gctcgaggtt
     2221 ctcgtgcacc gtcatcgacg ggaaggtgcc ccggccctgg agcagatagg cgatgccgcg
     2281 ccccagcatg tccttgggcg caaggcccgc gagcgaggcg ccgtcgtgga ggatgcggcc
     2341 gcgctcggga acgagatagc cgtaaatggt cttgagcagg gtcgacttgc cggcgccgtt
     2401 gggcccgatc agcgccgtca cccgccccga ctcggcgttg agcgcgaccc cgcgcaggat
     2461 cggcacctcg cgcgtgtagc cggcgaccag cccgtccacg gtcaaggtca tggcgccctc
     2521 aacggccgag ataggcctcg atcacggccg gatcggcgcg gatttccgcc ggcgtgccgc
     2581 gcgcgatcac gccgccgccg gcgagcacgg tgaccgactc gcacagcgcc gcaacctcgg
     2641 gaatctcatg gctgatcagc aggaaggtgg tgccgtgctc ggcattgagc gtgcgcacca
     2701 tggcgatcag ccgctcgacc agcgccgggg tgatgccgct gaacggctca tcgagcagca
     2761 ccagccgcgg ctcgcccatt acggtgcgca cgaactccag aagcttctgc tggccgcccg
     2821 agatctcgac cgccggcgcg tccgcaaggc cgtcgagctt ggcgagcgcg agcagcgcgc
     2881 gcgcccggtc ccgcgctttc gccccggtga gcccgcgcgg caccgccgcg accagcatgt
     2941 tgtcgagcac ggtcatctcg cggaacgcgc gcgcgacctg gaaggtgcgc cccacgccca
     3001 gcgccgcgac ccggctgagc ttgcggttgg tgatgtcgcg cccggccagc agcacccggc
     3061 ccgcgctcgc c
//
LOCUS       DASWHU010000361         7066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_613542, whole genome shotgun sequence.
ACCESSION   DASWHU010000361 DASWHU010000000
VERSION     DASWHU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613542"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGB88_13430"
     CDS             <1..393
                     /locus_tag="VGB88_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX9648500.1"
                     /translation="PLMGAGALDKALVEMWQRRVELNGFMMVGDAVRNSEPRFEGRGL
                     SGVPGGVPQIPAVVERGKAAALRLFDKLDNQLAGNEFVAGPRFTIADITLFATLWFAR
                     RAELAVPDACKNLTRWHAQVAARPSAEA"
     gene            complement(406..1251)
                     /locus_tag="VGB88_13435"
     CDS             complement(406..1251)
                     /locus_tag="VGB88_13435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX9648501.1"
                     /translation="MEDLPRLKARIANLGDLRDLIRALRALAASHLQEAQGALGGIRG
                     FVQAIEDSIAAGAALLDTAEQRDGARAPTGEAVAIVVCSEHGFVGAFNELLLDRAERE
                     LTAGSRGLAVIGRRGAMLAEERGLRVGWSLPMATHVGGVLAVTRRIAEQLATAEAVRI
                     VFGAYRRGRAVEVVARDVLPLDPGLLVRSARTSPPLHHLAPAVLLQRLAGEYLFAEIS
                     RAVMESLASENGARLGVMENADRNIADKLEAIQRRERTLRQESITAELIDVVTGAEAV
                     LGRKR"
     gene            complement(1253..2791)
                     /locus_tag="VGB88_13440"
     CDS             complement(1253..2791)
                     /locus_tag="VGB88_13440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9648502.1"
                     /translation="MTRPLRDDLAAWFPAARRRLDALQLAPALEHVGVVVQVGDGVAT
                     IAGLPLTRLDELLRFADGTLGLALRVDEESVGCVLLGAGEGVAAGTKVRGTGEIVRVP
                     VGEALLGRIVDATGAPLDDGAPIAAERLEPVERPAPAIVDRDLVTEPLMTGLVVVDAM
                     IPLGRGQRELIIGDRKTGKSAVAVDAVINQRSSDVICVYAAVGQKASTVNQVVEAVRA
                     HGAIERCIFVVGEANAPPAAQWLAPYAACTMAEYFRDRGRHALLVIDDLTKHAIIYRQ
                     LSLLLRKSPGREAYPGDVFFMHARLLERAAKLSDALGGGSLTALPIAETQAGNLTAYI
                     PTNLISITDGQIYLEPKLFYEGHKPAINVGMSVSRVGGQTQAPAIKQLAETLRLDYAQ
                     FLELEVFTRFGAMVDERIKRTIEHGRRIRAILTQAEFRPLPLVDQVALLLAVAERKLD
                     PVPLDAVERFKAEVGARLQEHCPRACARIEATGELSDEDRGDLVAALDDVIADVAGAR
                     GDGG"
     gene            complement(2772..3530)
                     /locus_tag="VGB88_13445"
     CDS             complement(2772..3530)
                     /locus_tag="VGB88_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648503.1"
                     /translation="MQISWWTLALQALNFLVLVWLLWRFLYKPVREVIDKRKALAEQA
                     FTDARRREQEAEAARRRLEDDRTSLARERQDLLRTLHQELEAERGNVLAAARADADAM
                     IAAATESIASERARALAESRDRIAGLAVDIAAGLLRDIAAAELDGVVLERIEAQLKEM
                     PTDELDRLRKDVAADGARLTVVTAKPIAPADQERWRGRFDACLGSGGKTEFATESGIV
                     GGAELRFPHAALKLTWADQLTKAKELLSGDQAAS"
     gene            complement(3533..3781)
                     /locus_tag="VGB88_13450"
     CDS             complement(3533..3781)
                     /locus_tag="VGB88_13450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9648504.1"
                     /translation="MELNLQMLSIIGAVITVSFGAIGPALGEGRAVAAAMDAIARQPE
                     SAGPLSRTLFVGLAMIETMAIYCLVIALLLLYANPFVG"
     gene            complement(3792..4463)
                     /gene="atpB"
                     /locus_tag="VGB88_13455"
     CDS             complement(3792..4463)
                     /gene="atpB"
                     /locus_tag="VGB88_13455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9648505.1"
                     /translation="MAEGPLAPDILFHLGPVPIDRAVATTWVIMAALSLVCGLGLRHA
                     RVHAGSLQTVLEIVIETLAKQINDIVRRDPWPYLPLLGTLFIFLVVANLSAVIPGAKP
                     PTGHIETPAALAAIVFLSVHVFGVRSRGLGDYLRHYTRPSLLLLPLNVLSEITRTFSL
                     MVRLFGNIMSHELIIAIVVFLAGLLLPIPFMLLGILIGIIQAYIFTVLAAVFIAAAVG
                     SAEAE"
     gene            complement(4466..4738)
                     /locus_tag="VGB88_13460"
     CDS             complement(4466..4738)
                     /locus_tag="VGB88_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX9648506.1"
                     /translation="MSATAPLDLVLGVVAGAAAGTLHFGLLWRAVRLHAQSAAASRIV
                     PLHLVRAGATVAVFWLVAQRGALPLLLALAGFLVARVVVQHRLRAE"
     gene            complement(4735..5025)
                     /locus_tag="VGB88_13465"
     CDS             complement(4735..5025)
                     /locus_tag="VGB88_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9648507.1"
                     /translation="MTLLHRNRGVRELQRAIRRRARRSERWQREGERSIGQNLAMIGA
                     LGWLIVTPILLGVLAGRWLDRAFATGITFSAALIFVGVVLGCYLAWRRIEKE"
     gene            complement(5022..5498)
                     /locus_tag="VGB88_13470"
     CDS             complement(5022..5498)
                     /locus_tag="VGB88_13470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX9648508.1"
                     /translation="MRLVVTTPVAVIVDADDVRHVRAEDETGAFGILAGHADFITTLA
                     ISVITWRNHAAEEHHVAVRGGVLTVQGGALVEVATRQAVGEDTLAKLGRAVLATLREE
                     AAAEEQSRVSATRLHLAAIRQLQRYLEAGRQPVPQGPPPELGAAFRGEPSGAGETR"
     gene            complement(5495..6952)
                     /gene="atpD"
                     /locus_tag="VGB88_13475"
     CDS             complement(5495..6952)
                     /gene="atpD"
                     /locus_tag="VGB88_13475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9648509.1"
                     /translation="MSGVAKQADPEGATPTAPPLGRVTSVSGSVVEMAFPAGRLPAIN
                     AAVEIAWDGPHRLVVEVHQHVDPRTVRGVAMRATSGLACGVGARDTGAPIRVPTGTAV
                     LGRLIDVLGEPIDHGPDFAADVPRAPIHRGPPRLTEQSADRKLFLTGIKVMDLLAPLA
                     RGGKAAMFGGAGVGKTVLIMEFIRTTVEQHAGISVFAGIGERSREGHELWLELKRAGV
                     LMRTALVFGQMSEPPGARWRVGMTALTIAEYFRDVMHQNVLLLIDNVFRFVQAGGEVS
                     GLLGRLPSRVGYQPTLATEIAELEERIASVKDAAVTSIQAVYVPADDFTDPAVAETFT
                     HLDSSIVLSREMANQGLYPAIDPLASSSVMLDARLVGEAHYRTAEEVRRLIEQYRELR
                     EIISLLGMEELSPADRTAVGRARRLVRFLTQPFAVTAQFTGRAGASVPLEETLAGCRA
                     ILDGEADGWAESSLYMVGTLDDARAKEAGTAQAAS"
BASE COUNT         1029 a         2585 c         2487 g          965 t
ORIGIN      
        1 ccgctgatgg gcgcaggcgc gctcgacaag gcgctggtcg agatgtggca gcgccgggtc
       61 gagctcaacg gcttcatgat ggtcggcgac gcggtgcgca actccgagcc gcgcttcgag
      121 ggccgcgggc tgtcgggcgt gcccggcggc gtaccgcaga tcccggcggt ggtcgagcgc
      181 ggcaaggcgg cggcgctgcg cctgttcgat aagctcgata atcagttggc aggcaacgag
      241 ttcgtcgccg ggccgcgctt caccattgcc gacatcacgc tgttcgcgac cctatggttc
      301 gcccggcgcg ccgagcttgc ggtccccgat gcgtgcaaga acctgacgcg ctggcacgcc
      361 caggtcgccg cgcgcccgag cgccgaggcg tgaccccgcg ccgggtcagc gcttgcgccc
      421 gagcacggcc tcggcgccgg tgaccacgtc gatcagctct gccgtgatcg actcctggcg
      481 gagggtgcgc tcgcggcgct gtatcgcctc cagcttgtcg gcgatgttgc ggtccgcgtt
      541 ctccatcacc ccgagccgcg cgccgttctc gctcgccagc gactccatga ccgcgcggct
      601 gatctcggcg aacaggtatt cgcccgccag ccgctgcaac agcaccgccg gggcgagatg
      661 atggagcggc gggctggtgc gcgccgacct caccagaagc cccggatcga gcggcaggac
      721 gtccctggcc acgacctcga ccgcgcggcc cctgcggtag gcgccgaaca cgatgcgcac
      781 cgcctcggcc gtcgccagct gctcggcgat ccttcgcgtc actgcgagca cgcccccgac
      841 atgtgtcgcc atcggcaggc tccagccgac cctgagcccg cgctcctcgg cgagcatcgc
      901 gccgcgccgg ccgatcacgg caaggccgcg tgagccggcg gtgagctcgc gctcggcgcg
      961 gtcgagaagc agctcgttga aggcgccgac gaagccgtgc tccgagcaga cgacgatcgc
     1021 caccgcctcg ccggtgggcg cgcgcgcgcc gtcgcgctgc tccgccgtgt cgagcaatgc
     1081 ggcgcctgcc gcgatcgagt cctcgatcgc ctgcacgaag ccgcgtatcc cgcccagcgc
     1141 gccctgcgcc tcctggagat gcgaggcggc gagcgcgcgc agcgcccgga tcaggtcgcg
     1201 caggtcgccg agattggcga tccgcgcctt cagccgcggc aggtcctcca tgtcaaccgc
     1261 cgtcgccgcg cgcgccggcg acgtcggcga tgacgtcgtc gagcgccgcg accaggtcgc
     1321 cccggtcctc gtcgctgagc tcgccggtcg cctcgatgcg cgcacaggcg cgcgggcaat
     1381 gctcctgaag ccgcgcgccg acctcggcct tgaaccgctc gaccgcatcg agcgggaccg
     1441 ggtcgagctt tcgctccgcc accgccagca gcaacgccac ctgatcgacc agcggcaacg
     1501 gccggaactc cgcctgggtg aggatcgcgc ggatgcgccg gccgtgctcg atggtgcgct
     1561 tgatccgctc gtcgaccatc gcgccgaagc gcgtgaacac ctcgagctcg aggaactgcg
     1621 catagtcgag ccgcagggtt tcggccagtt gcttgatcgc cggcgcctgg gtctgcccgc
     1681 ccacccgcga gacgctcatg ccgacgttga tcgccggctt gtgcccctcg tagaacagct
     1741 tgggctcgag atagatctgg ccgtcggtga tcgagatcag gttggtcggg atgtaggcgg
     1801 tgagattgcc cgcttgcgtc tccgcgatcg gcagcgccgt gagcgagccg ccgccgagcg
     1861 cgtcagagag cttggcggcg cgctccaaca gccgcgcgtg catgaagaac acgtcgcccg
     1921 gataggcctc gcggcccggc gacttcctga gcagcagcga cagctggcga tagatgatgg
     1981 cgtgcttggt caggtcgtcg atgaccagca gcgcgtggcg cccgcggtcc ctgaaatact
     2041 ccgccatggt gcacgccgca tagggcgcga gccactgcgc ggccggcggc gcgttcgcct
     2101 cgccgaccac gaagatgcag cgctcgattg cgccgtgggc gcgcaccgcc tcgaccacct
     2161 ggttgaccgt cgacgccttc tggccgacgg cggcatagac gcagatcacg tcggaggagc
     2221 gctgattgat gaccgcatcg accgcgaccg cgctcttgcc ggtcttgcgg tcgccgatga
     2281 tcagctcgcg ctggccgcgc ccgagcggga tcatggcgtc gaccaccacc agcccggtca
     2341 tcagcggctc ggtgaccagg tcgcggtcga cgatcgccgg cgccggccgc tcgacgggct
     2401 cgaggcgttc ggccgcgatc ggcgcgccgt cgtcgagcgg cgcgccggtg gcatcgacga
     2461 tccgccccag cagcgcctcg cccaccggca cgcggacgat ctcgcccgtg ccgcggacct
     2521 tggtgccggc ggcgaccccc tcgccggcgc cgagcaggac gcagccgacc gactcctcat
     2581 cgacccgcag cgcgaggccg agcgtgccgt ccgcgaaccg gagcagctcg tcgagccggg
     2641 tcaggggcag ccccgcgatc gtcgccacgc cgtcgccgac ctgcaccacc acgcccacat
     2701 gctcgagcgc gggcgcaagc tgcagcgcgt ccagccggcg gcgggcggcg gggaaccagg
     2761 cggcgaggtc gtcacgaagc ggcctggtca ccgctcaaca gctccttggc tttggtcaac
     2821 tggtcggccc aggtcagctt cagcgccgca tgcgggaagc gcagctcggc gccgccgacg
     2881 atcccggatt cggtcgcgaa ctcggtcttg ccgccgctgc cgagacaggc atcgaagcgg
     2941 cctcgccacc gctcctggtc cgccggcgca atcggcttgg cggtgaccac ggtcaggcgc
     3001 gcgccgtcgg cggcgacgtc cttcctgagc cggtcgagct cgtccgtcgg catctccttc
     3061 agctgcgcct cgatgcgttc caggacgacg ccgtcaagct ccgccgcggc gatgtcgcgc
     3121 agcaggccgg cggcgatgtc caccgcgagg ccggcgatgc ggtcgcggct ctcggcgagc
     3181 gcacgcgcgc gctccgaggc gatcgactcc gtcgccgccg cgatcatggc gtcggcgtcc
     3241 gcgcgcgccg cggcgaggac gttgccgcgc tcggcctcaa gctcctggtg cagagtcctg
     3301 agcaggtcct ggcgctcgcg ggcgaggctc gtgcggtcgt cctcgagccg ccgacgcgcc
     3361 gcctccgcct cctgctcgcg gcggcgcgcg tcggtgaacg cctgctcggc gagcgccttg
     3421 cgcttgtcga taacctctct gaccggcttg tacaggaacc gccagagcag ccagacgaga
     3481 accaggaagt tgagcgcctg cagcgcaagc gtccaccagc ttatctgcat cgtcagccga
     3541 cgaacgggtt ggcgtagagc agcagcagcg cgatcaccag gcagtagatc gccatggtct
     3601 cgatcatcgc caagccgacg aacagcgtgc gcgacagggg ccccgcagat tcgggctggc
     3661 gggcgatcgc gtccatcgcc gcggcgaccg cgcggccttc gccgagcgcc gggccgatgg
     3721 cgccgaacga gaccgtgatc accgcgccga tgatgctcaa catctgaagg tttagttcca
     3781 tggggcctcc gtcattccgc ttccgcgctg ccgaccgcgg cggcgatgaa caccgccgcc
     3841 agaacggtga agatgtaggc ctggatgatg ccgatcagga tgccgagcag catgaacggg
     3901 atcggcagca gcaggcccgc caggaacacc acgatcgcga ttatgagctc gtggctcatg
     3961 atgttgccga acaggcggac catgagcgag aaggtgcggg tgatctcgga cagtacattc
     4021 agcggcaaca gcagcaggct gggcctggtg tagtggcgga gatagtcgcc gagcccgcgc
     4081 gagcggacgc cgaagacgtg gaccgacagg aacacgatcg cggccagcgc cgccggcgtc
     4141 tcgatatgcc cggtcggcgg cttcgcgccg gggatcaccg ccgacaggtt ggcgaccacc
     4201 aggaagatga acagcgtgcc gagcagcggc agatacggcc acgggtcgcg gcggacgatg
     4261 tcgttgatct gcttggccaa ggtctcgatc acgatctcga gcacggtctg caagctgccg
     4321 gcgtgaaccc gcgcgtggcg gagccccagc ccgcagacca ggctcagcgc cgccatgatc
     4381 acccaggtcg tcgccaccgc gcggtcgatc ggcaccgggc cgaggtggaa caggatgtcg
     4441 ggagcgagcg ggccttccgc catgatcact cggccctcaa gcggtgctgg accacgacgc
     4501 gcgcgaccag gaagccggcg agcgcgagca gcagcggcag cgcgccccgc tgcgcgacca
     4561 gccagaatac ggccaccgtc gcgccggccc gcaccaggtg cagcggcacg atccggctcg
     4621 ccgccgccga ctgcgcgtgc agccgcaccg cccgccacag cagcccgaag tggagcgtgc
     4681 cggccgcggc gccggcaacg acgccgagaa ccaaatcgag cggggccgtc gcgctcattc
     4741 cttctcgatc cttcgccagg caagatagca gccgagcacg acgccgacga agatcagcgc
     4801 ggcgctgaag gttatgccgg tcgcgaacgc gcggtcgagc cagcgcccgg caagcacgcc
     4861 gagcaggatc ggcgtcacga tcagccagcc gagcgcgccg atcatcgcca ggttctggcc
     4921 gatcgagcgc tcgccctcgc gctgccagcg ctcgctgcgc cgcgcccggc ggcggatcgc
     4981 gcgctgcagc tcgcgcacgc cgcgattgcg gtgcagcagg gtcatcgcgt ttcccccgcc
     5041 cccgacggct cgccgcggaa cgccgcgccg agctcgggcg gcggcccctg cggcaccggc
     5101 tggcgtcccg cttccaggta cctttgcagc tggcggatcg cggccaggtg caggcgcgtg
     5161 gccgagacgc gcgactgctc ctccgccgcg gcctcctcgc gcaacgtggc gagcacggcg
     5221 cggccgagct tggcgagcgt gtcctcgccg accgcctggc gggtggcgac ctcgaccagc
     5281 gcgccgccct gcaccgtcag cacgccgccg cgcaccgcga cgtgatgctc ctccgcggcg
     5341 tggttgcgcc aggtgatcac cgagatcgcg agcgtggtga tgaagtccgc gtgcccggcc
     5401 aggatgccga acgcgccggt ctcgtcctcg gcgcggacgt ggcggacgtc gtcggcatcg
     5461 acgatcaccg cgaccggcgt ggtcacgacc agcctcatga cgcggcctgg gcggtgcccg
     5521 cttccttggc gcgcgcgtcg tcgagcgtac cgaccatgta gagcgagctt tccgcccagc
     5581 cgtcggcctc gccgtcgagg atcgcccggc agccggcgag cgtctcctcg agcggcaccg
     5641 acgcgcctgc gcgcccggtg aactgggcgg tgaccgcgaa cggctgggtc aggaacctga
     5701 ccagccggcg cgcgcgcccg accgcggtcc ggtcggcggg gctgagctct tccatgccca
     5761 agagcgagat gatctcgcgc agctcgcggt attgctcgat caggcgccgg acctcctcgg
     5821 cggtccggta atgggcctcg ccgaccaggc gcgcatcgag catcaccgag gacgacgcca
     5881 gcgggtcgat cgccggatac agcccctggt tcgccatctc gcggctgagc acgatcgagg
     5941 aatcgagatg ggtgaaggtc tcggcgaccg ccgggtcggt gaagtcgtcg gccggcacgt
     6001 agaccgcctg gatcgatgtc accgcggcgt ccttgaccga ggcgatgcgt tcctcgagct
     6061 cggcgatctc ggtggccaag gtcggctggt agccgacccg cgacggcagc cggccgagca
     6121 gccccgagac ctcgccgccc gcctggacga agcggaacac gttgtcgatc agcagcagca
     6181 cgttctggtg catcacgtcg cggaagtact cggcgatcgt gagcgcggtc atgcccaccc
     6241 gccagcgcgc gcccggcggc tcgctcatct ggccgaacac cagcgcggtg cgcatcagca
     6301 cgccggcgcg cttgagctcg agccacagct cgtggccttc gcgcgagcgc tcgccgatgc
     6361 cggcgaacac ggagatgccg gcgtgctgct cgaccgtggt gcggatgaac tccatgatca
     6421 gcacggtctt gccgacgccg gcgccgccga acatcgccgc cttgccgccg cgcgcgagcg
     6481 gcgccagcag gtccatcacc ttgatgccgg tgaggaacag cttgcggtca gcgctctgct
     6541 cggtcagccg cggcgggcca cggtggatcg gggcgcgcgg cacgtcggcg gcgaaatcgg
     6601 gcccgtggtc gatcggctcg ccgagcacgt cgatcagccg cccgagcacg gccgtgccgg
     6661 tcggcacccg gatcggcgcg ccggtatcgc gcgcgccgac gccgcacgcg aggcctgagg
     6721 tggcgcgcat ggccacgccc cgcaccgtgc gcggatcgac gtgctggtgt acctcgacca
     6781 ccagccggtg cgggccgtcc cacgcgatct cgaccgcggc gtttatcgcc ggcaggcggc
     6841 cggcgggaaa ggccatctcg accaccgagc cgctgacgct ggtgacgcgg ccgagcggcg
     6901 gcgccgtggg cgtggcgcct tccggatcag cctgcttcgc gacaccgctc agggccaggt
     6961 cctccggctc agatcgccgt catcgggtgc gggcacgggc cccacctaag aggattcgcc
     7021 cgcccttgtc gatgcgattc ggtcgccgca cttgcgcccg cgcgca
//
LOCUS       DASWHU010000362        19177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_613689, whole genome shotgun sequence.
ACCESSION   DASWHU010000362 DASWHU010000000
VERSION     DASWHU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19177
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613689"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGB88_13480"
     CDS             <1..773
                     /locus_tag="VGB88_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9648510.1"
                     /translation="PEEPRTTADAADRRRGEQGAQWFRRLGALGVPIDWVQAQVEASE
                     LAPGVWHLTGGTHHSLAIALDDGIVLVEPPLYEARSQAVIAKVAELWPGKPIKTVVAT
                     HHHFDHLGGVRSFAAAGAEVVIGAAAEAFLHQVMERPHTLVPDALQQQPRAVAIRAVG
                     EAPLALGDAHREVLAVPIPARHAADMVAVYLPGEKLLFNSDMYTPGRGGPLNELAQRN
                     GEDLRQAIADHGLAVETIVGGHGVGTVSYADFLAHLGG"
     gene            complement(783..1418)
                     /locus_tag="VGB88_13485"
     CDS             complement(783..1418)
                     /locus_tag="VGB88_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010447714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX9648511.1"
                     /translation="MYRPAAFDEPDVGRLHRLIDDIAIAILVSAGADGLEASHLPVLL
                     DAGAGPHGTILGHLARANPHWRRLDGGEVLLVLQGPNGYVSPNWYPTKQETGMVVPTW
                     NYVAVHVTGEARTFDDRDSLRDLVTRLTERHERRFAAPWSVADAPAGFVDRMLGQIVG
                     VAVRVRRLEGKLKLSQNRPPEDRQGVVAGLDATGDAGDALLAAAMRRLSEI"
     gene            complement(1421..1837)
                     /locus_tag="VGB88_13490"
     CDS             complement(1421..1837)
                     /locus_tag="VGB88_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9648512.1"
                     /translation="MSAPIRLRGLDHLVLRTARLESMIAFYRDALGCAVERRLDDLGL
                     VQLRAGASLIDLIDVAGTLGKEGGAAAGPTARNLDHFCLQLAEFDAGAIARHLGAHGA
                     AVGEVTRRYGAEGFGPSVYFKDPDGNMVELKGPAEG"
     gene            complement(1834..2712)
                     /locus_tag="VGB88_13495"
     CDS             complement(1834..2712)
                     /locus_tag="VGB88_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX9648513.1"
                     /translation="MSAGEKRKRLRRRLGEAPVIVAPGVFDMISARIADGLGFDALYM
                     TGYGVSASHLGLPDAGFATLTDMAERVRRIADGTGAPLIADADTGYGGALNVRHTVRT
                     YEAAGAAAVQIEDQEFPKRCGHTTGRRVIPLADMVTKVKVAADARTDADLLIVARTDA
                     RTAYGLDEAIDRAAAYAEAGADVLFVESPESEDEMRRICQSLGKPVLANMVEGGRTPL
                     LGAERLGDIGYRIAIYPATGFLAAAAALASVYGTLRRSGSSAAVEVPLYPFEEFNRMI
                     GFEEVWDFDRTYGGEG"
     gene            2806..4779
                     /locus_tag="VGB88_13500"
     CDS             2806..4779
                     /locus_tag="VGB88_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9648514.1"
                     /translation="MTRPETPYPRLFSPLDLAGRTLRNRIVHLATVTNFNDAGRITDR
                     FIAYHAARAKGGAGMIVTEGLSVHPSSAPNPRVVALYDPANRDGLKRLAGAVEGAGCR
                     LLGQLWHVGRQQLWGPQATPLGVSHLPDALSWQVPHVMDRSQIEALVAAFVACARLLQ
                     EAGFSGAELHGAHGYLITQFLSPWSNLRDDDYGGDRVRRMRFLSEVIAGIRAACGARF
                     ILGLKMPAEEGVAGGIDLAEARAITTALAADGGLDYIAYSQGNFSLSLERHLPDMHFP
                     SAPYAALARALKPAAGAIKVMALARIASAGEAEALLADGAGELVGLGRPLIAEPAWPD
                     KLARGDGAELRPCTYCNYCWSEVYAGRALACAQNVRLGGTQELAPWIAPAKWRRVAVV
                     GAGPAGLEAAWLAASAGHHVTLFGASEAPGGKVRIEALLPGRGEAMEVVRFQAERIAR
                     LGVEQRLGRAASADDIAAAAPEVAVLATGATMQPPPLAGGSAAMAALTDAVPGLVADA
                     APRAGTAVLFDHDQSPPVYAAAELLAARYARVVIVTPATQIARHVPYVSAIGVHRRLF
                     AAGVEIVIGCRPVAWTDGRLVLENLFTGAETAIADVACALYATPRRANDALAAPLAEA
                     GIALHLIGDAYAPRTLHAAMLEARRAAEKIGSS"
     gene            complement(5028..6044)
                     /locus_tag="VGB88_13505"
     CDS             complement(5028..6044)
                     /locus_tag="VGB88_13505"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9648515.1"
                     /translation="MERVRRFVDSSDIVADGPALARRMAQDGYLFIPALVPAADIAAV
                     RSQVLDVVAEAGWLGAEDARADAIANPDAACADPDDRYVPVLKRIYRIEALHALTHHP
                     ATLGLFARMFADQVLVHPMLIPRNIFPQRPELTTPAHQDFPHIQGTPETFAVWLPLSD
                     CPLAMGGLAVAEGSHAQGVHEFRVSSGAGAMEVCDPLDGRWVSGDFAQGDAVIFHSMT
                     VHRALPNMTSRLRQSVDARYQRLGEPVAERSLAPYAGTGSWDQIYRGWRSSEFQYYWR
                     RRPLSLAPFDWQYYERRDQMAFEIAANGDPVARATLLRIIQRDPDPAKREHAARLVAA
                     LDGA"
     gene            complement(6052..6933)
                     /locus_tag="VGB88_13510"
     CDS             complement(6052..6933)
                     /locus_tag="VGB88_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9648516.1"
                     /translation="MTRRGARAGREGMEYLSTLPRRVVTIYVPLAVFVIVLLFPFYWM
                     AITSVKSNQELLDFEAGNPFWVSSPTLAHINKLLFETSYPAWLWNTILVSVVATAFSI
                     FASVLAAYAITRLRFRGGNLVGVAIFLAYLVPPSILFIPLSLMVVQLGVFDTRLALMF
                     TYPTILIPFSTWLLMGYFKTIPYELEECALVDGASRLQVLVRIILPLAVPGLISAGIF
                     AFTLSWNEFIYALTFISSSEVKTVPVGVVTELVEGDVYHWGSLMAGALFGSLPIAILY
                     AFFVEHYVSSMTGAIKE"
     gene            complement(6941..7900)
                     /locus_tag="VGB88_13515"
     CDS             complement(6941..7900)
                     /locus_tag="VGB88_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9648517.1"
                     /translation="MIDRAQELAAPGPDVIGLGRRLARLRDSKALLSFLCMLPAAAVL
                     VGFLAYPLGLGIWLGFTDSTIGRPGIFIGVENFVFLADDPVFWLSVWNTILYTTVASV
                     FKFALGLWLALILNNRIPFKAFLRAIVLLPWIVPTVLSAIAFWWLYDAQFSIISYSLE
                     KLGLIDRYINFLGDPNNARASVIAANIWRGIPFVAITLLAGLQTISPSLYEAAVIDGA
                     GSWQRFRYVTLPQLTPIIAVVMTFSVLFTFTDFQLIYVLTRGGPLNATHLMATLSFQR
                     AIPGGQLGEGAAISTAMIPFLLACIMISYFGLQRRRWQQGTND"
     gene            complement(7908..9233)
                     /locus_tag="VGB88_13520"
     CDS             complement(7908..9233)
                     /locus_tag="VGB88_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9648518.1"
                     /translation="MATITRRGVLTGGAGLAAAGALGLGPLGRAARAQELKFTPEAGA
                     ELRVLRWKRFVEGDETVFTANTARFTEITGVPVRVDNESWEDLRPKAAVAANVGKGPD
                     IILGFNDDPHQFPDKLIDVNDLADYLGAKYGGWYDVCRTYGTKGERWIALPMGGPTSC
                     TVYRRSWLAEAGFEALPGDNAGFLAACQALAAKGHPPGFAFGHAVGDGNAVCQWLLWS
                     HGGMIVDENDQVVLERPETLAALEYAKELYATLIPGTISWLDPHNNKAFLSEQIGLTF
                     NGISIYYAAKNSEEAKLKAIAEDIEHANLPVGPVGRPTQGALVTLAMIFSYSPYPNAA
                     KEYLRFLMEKDQYDPWQQASIGYITQPLAAYESNPVWTEDPKHTPFRDNLKRALWDGF
                     AGSSGYASAATAADFVLVDMFAEVVAGQRTPKEAAQRAQRRAERYYRRT"
     gene            complement(9295..10368)
                     /gene="ugpC"
                     /locus_tag="VGB88_13525"
     CDS             complement(9295..10368)
                     /gene="ugpC"
                     /locus_tag="VGB88_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9648519.1"
                     /translation="MATVEARGVRKMFGATEVLHGIDIDIRDEEFVVLVGPSGCGKST
                     FLRMVAGLEEVTGGEILIDRRVVNAMPPKERNVAMVFQNYALYPHMTVYGNMAFSLKL
                     ARVDRGEIERQVRRAADILGLEEYLDRYPRQLSGGQRQRVAMGRAIVRDPAVFLFDEP
                     LSNLDAKLRVQMRTEIKALHQRLRTTSVYVTHDQVEAMTMADRIVVLNDGRVEQVGEP
                     LAIYDRPDNLFVAAFIGSPAMNFITGRLRRDGGARAWVEAADGTALPLQAPAPERADG
                     AVVFGVRPEHLELADGEGGIACKVSVVEPTGADTLVFARMAGIDICAVFDERHQFKPG
                     QVIHLSPRAGTVHLFDAATGGHL"
     gene            complement(10469..11293)
                     /locus_tag="VGB88_13530"
     CDS             complement(10469..11293)
                     /locus_tag="VGB88_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX9648520.1"
                     /translation="MVEASPGALRVGIAGLGAIGMAVARRLDAGIDGLALVAVAARDL
                     ARARARVADFRAPPEVCAAPRLAEHAEVVVECAPAAAFRAIAEPVLAAGRTLVPASVG
                     QLLAHPDLVELARRSGARIVVPTGALLGLDAVRAAAEGEITAVTMVTRKPPASLAGAP
                     YLAEAGISLDGLTAPLKVFAGSAGEGARGFPANVNVAAALSLAGIGPERTRLEVWADP
                     ALSRNVHHIEVEADSARFSMTIENVPSADNPRTGRITALSIVALLRRLVSPLTVGS"
     gene            11378..12343
                     /locus_tag="VGB88_13535"
     CDS             11378..12343
                     /locus_tag="VGB88_13535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9648521.1"
                     /translation="MRTAGWAIRVLAAAVLWTGAGAAGALDMPCRTARLIVPWAPGGE
                     TAINFELIADAVNRSGVSPRLQVVTIPGQGGNKGAKVARDARPDGCTLFALHQSAITS
                     YLSGRVDFTWDAFEPVALLTGTPSMIGARPGASYADLAGLIAAARAAPQTVLAGSTLG
                     STSHFLFLLLEKAAGIKLKHVSYDGTRERLTALLAQTIDVGEINVVSAKEYLDDDALK
                     ALGIATAARDPLAPEVPTLAEQGVDITFGTDRGVVLPKGAAGEVVAFWERAFLDALAG
                     PELRRALEAKGNTIIGQGAEAYRAALAAEFAALEAAAAEAGLYRR"
     gene            12361..12831
                     /locus_tag="VGB88_13540"
     CDS             12361..12831
                     /locus_tag="VGB88_13540"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9648522.1"
                     /translation="MTCDSAAALGLVALCAALIAATFDIRETNYATPGAEVWPRIVLA
                     WLSALTLVYLARSLVRARRARGPEAEPRPGLPANVAWCFALFAAFLLALPYAGMLVAG
                     IGFVFAALCVMGRRDRRALARHAAIAVASVGTVWAAFTYALGVMLPAGELLGAW"
     gene            12851..14503
                     /locus_tag="VGB88_13545"
     CDS             12851..14503
                     /locus_tag="VGB88_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9648523.1"
                     /translation="MQGADILAAAASVLDPLTLALLAIGVAAGLIAGAIPGFTIAMAV
                     VLTLPFTFGMAPIQGLATMIGVFVGGLSGGLMAAILTGIPGTPSAVATTFDGFPMARG
                     GRPGLALGIGVWASFAGGLISAVILVTLAPVLARIGLEFGPWDYFALIAFALTITASL
                     AGEDILKGLIAGALGLLAATVGEDNINGVARFTFGIDALHQGFAYLPVLIGLFAFSQL
                     MSDVADPVRARSPLLDVPRAAVRIRHRQAIRTVAGEWATLVRSSLIGVFTGILPAAGS
                     SISNILAYDQARKASAHPEQFGTGIPAGIVASESANNATAGGALITMMALGIPGDIVT
                     AVMLGALLIHDVVPSPSFIADAPVLAYGIFIAFFLAHFVMIALQAVCLRGFLLVTRVP
                     MYVLAAVILAYCAVGVFALNNVDFDLWTLLLFGLAGTGLRALGLPLAPMILGVVLGTT
                     AEVNLSRALTISDGAAQAAADFVTRPWSLLFLILAAFSAAFPWYQAARAHARPWTRLY
                     LPAMAVAVSGPLFLMDGWFRPLVGAGLLVAGPWLVWRRLVRA"
     gene            14640..16421
                     /locus_tag="VGB88_13550"
     CDS             14640..16421
                     /locus_tag="VGB88_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9648524.1"
                     /translation="MASGGFDFCLGIGGAAGQGIATPGNVLARIFARRGIHLSAYNAY
                     QSIIRGGHILLTMRASAEPRHSHGDRLDLLICLNQDTMDRHLGLMGKGARVVFNADAI
                     KPGKAASGVQMCPLPVAELSGNSRNKLVQNVASLGAVASLLGLSFDILEAALSQQFGG
                     KGGGAVDENVAVARAAFDHAAASFEPLAEPIPEGTRPLGVWTGNDALAMGGAAAGVKF
                     YCAYPMSPSTGVLHWMARHARDLGIMVRQGEDEIAVANMVIGAAQVGCRAMCATSGGG
                     FALMTEAIGSAGMMEIPAVFINVQRAGPSTGVPTKTEQGDLWQALGASQGDFQRFIVA
                     PTDALDGFNTLPELFNLCDRHQCPGILISDLLISEGTFTVDPEALDLQPAIERGEVIT
                     EGRVDGAYRRYLDTDSGISPRALPGVEGYVHVVATDEHDQDGVLISDEFTNPIKRRQM
                     VEKRARKFDGVAERIAPPALIGPADADVTLVGWGSTKGVIRDAMAALADAGISANQLP
                     VKWIVPFHAAEVGRILAEAKRVVVVENNHSGQFYRYLRSETGLAVDGHIRKYDGEPFM
                     PHHIVEGVQALLAGETDRYVPEHEIMV"
     gene            16432..17334
                     /locus_tag="VGB88_13555"
     CDS             16432..17334
                     /locus_tag="VGB88_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9648525.1"
                     /translation="MAGVAIEAVEAERLTAKDFKGKVDPDWCKGCGDFGVLNALQRAC
                     AELGLKPHEILVVSGIGCSSNLPGYFNAYGMHTLHGRTLAVATGAKLANHALTVIATG
                     GDGDGFGIGGNHFTHTARRNVDLTYIVMDNQIYGLTTGQVSPTSMRKMRTKSTPFGSV
                     EGPLNPITSAIMNGATFVARGFSGDPKYLSDMIMRAIRHRGFAFIDVFSPCVTFNHDN
                     DFAFFKERVKRLDDDGHDRSDWKAACEKAMVWDDVIYTGVFLERTDEPSLGEQEPVLA
                     AGPLSSRPPGLTKEQANGIIARMM"
     gene            complement(17353..18279)
                     /locus_tag="VGB88_13560"
     CDS             complement(17353..18279)
                     /locus_tag="VGB88_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648526.1"
                     /translation="MTRARRSAFAVALLVAAGCRAGGAGEDDLAYVSDYYSFVGADAA
                     GRVAFALDANRGRDGDDFQAELFAALYDDAAGWVDLAGSGPYANAARVLDDVPESADF
                     RYSGTPADGVTILSEPNALTLTTRPIEIRMSDSTGARARRLGSAAATLAWQGRRLDGR
                     VIYEFVRFDGWNRLARTYWSTWNDWQSFYLVAGETVGAGGDDVYLQSQDADGRREVDG
                     FAVLNGRARRLADAELTVEDSDQALGVYRWASAWSGGWPDAPAARLTLVARDRRSFGN
                     WLIGGFAMTLVTGELTVGGRAVPVWGLGELIK"
     gene            complement(18291..19052)
                     /locus_tag="VGB88_13565"
     CDS             complement(18291..19052)
                     /locus_tag="VGB88_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648527.1"
                     /translation="MDLGLGGLKAVVTGGTRGIGRAIAELLAGEGCHVGICARGGGAI
                     APAVAALKAKGVNATGGAADVRDAAALKAWCDAAAAELGGIDILVANVSGFGTSLDEA
                     GWRQAFDGDILGTFNAVEAALPYLERSHAGAIVAISSVAALEAFGGVRPYNALKAALI
                     TYVSNLAHELAAKGVRANSVSPGTIYFKGGVWHQREQQSPELFKRALARNPMGRMGTP
                     DEVARSAAFLASPAASFVTGTNLIVDGCLTQRVQF"
BASE COUNT         2738 a         6797 c         6920 g         2722 t
ORIGIN      
        1 tgcccgagga gccgcgcacg acggccgatg ccgcggaccg caggcgcggc gagcagggcg
       61 cgcagtggtt ccgccgcctc ggcgcgctcg gcgtgccgat cgactgggtg caggcgcagg
      121 tcgaggcgag cgagctcgcg cccggcgtct ggcacctcac cggcggcacc caccattcgc
      181 tcgccatcgc gctcgacgac ggcatcgtgc tggtcgagcc accgctttac gaggcgcgct
      241 cgcaggcagt gatcgcgaag gtggccgagc tgtggccggg caagccgatc aagaccgtgg
      301 tcgcgaccca ccaccacttc gaccatctgg gcggcgtgcg cagcttcgcc gccgcgggcg
      361 cggaggtggt gatcggcgcg gccgccgagg cgttcctgca ccaggtgatg gagcgcccgc
      421 ataccctcgt cccggacgcg ctccagcagc agccgcgcgc ggtcgccatc cgcgcggtcg
      481 gcgaggcgcc gctcgcgctc ggcgacgcgc accgcgaggt gctggcggtg ccgatccccg
      541 cccgccacgc cgccgacatg gtggcggtct acctgccggg ggagaagctg ttgttcaaca
      601 gcgacatgta caccccgggg cggggaggcc cgctgaacga gctggcgcag cgcaacggcg
      661 aggacctgcg tcaggcgatc gcggaccacg gcctcgcggt cgagacgatc gtcggcggcc
      721 acggcgtggg caccgtgtcc tacgccgact tccttgccca tctgggcggc tgagggcgcg
      781 gctcaaatct cgctcagccg gcgcatcgcc gcggcgagca gcgcatcgcc ggcatcgccc
      841 gtggcatcga ggccggcgac cacgccctgc cggtcctcgg gtgggcggtt ctggctgagc
      901 ttgagcttgc cctcgaggcg cctgacgcgc accgccacgc cgacgatctg gccgagcatg
      961 cggtcgacga agccggccgg cgcgtccgcg accgaccacg gcgcggcgaa gcgccgttcg
     1021 tggcgctcgg tgagccgcgt gaccaggtcg cgcaatgagt cacggtcatc gaaggtgcgc
     1081 gcctcgccgg tcacgtggac ggcgacgtag ttccaggtcg gcaccaccat cccggtttcc
     1141 tgcttggtcg ggtaccagtt gggcgagacg tagccgttcg gcccttgcag gaccagcagc
     1201 acctcgccgc cgtcgagccg gcgccaatgc gggttggcgc gggcgaggtg gccgaggatg
     1261 gtgccgtgcg gccccgcgcc cgcgtccagc agcaccggca ggtggctcgc ctcgagcccg
     1321 tccgcgccgg ccgagaccag gattgcgatg gcgatgtcgt cgatcagccg atgcagccgg
     1381 ccgacatccg gctcgtcgaa cgcggccggg cggtacaccg tcagccctcc gccgggccct
     1441 tgagctcgac catgttgccg tccgggtcct tgaaatagac cgacggcccg aagccctcgg
     1501 cgccgtagcg gcgtgtcacc tcgcccaccg cggcgccgtg cgcgccgagg tgccgggcga
     1561 tcgcgccggc gtcgaactcg gcgagctgga ggcagaagtg gtcgaggttg cgcgcggtcg
     1621 gccccgccgc ggcgccgccc tccttgccga gcgtgccggc gacgtcgatc aggtcgatca
     1681 ggctcgcccc ggcgcggagc tgcaccagcc cgaggtcgtc gagccgccgc tcgaccgcgc
     1741 agccgagcgc gtcgcgatag aacgcgatca tcgactcgag ccgggcggtg cgcagcacca
     1801 ggtggtcgag tccgcgcaac ctgatcgggg cgctcacccc tcgcctccat acgtgcggtc
     1861 gaagtcccat acctcctcga agccgatcat gcggttgaac tcctcgaagg gatagagcgg
     1921 cacctcgacc gcggcgctcg acccggagcg cctcaaggtg ccgtagaccg aggccaatgc
     1981 cgcggcggcg gcgaggaagc cggtcgcggg atagatcgcg atgcgatagc cgatatcgcc
     2041 cagccgctcg gcgcccagca gcggcgtgcg cccgccctcg accatgttgg cgagcacggg
     2101 cttgcccagg ctttggcaga tgcggcgcat ctcgtcctcg ctctcgggcg attcgacgaa
     2161 cagcacgtcg gcgccggcct cggcgtaagc cgcggcgcgg tcgatcgcct cgtcgaggcc
     2221 gtaggcggtg cgcgcgtcgg tgcgggcgac gatcaacaaa tccgcgtcgg tgcgggcatc
     2281 ggccgcgacc ttgaccttgg tcaccatgtc ggcgagcggg atcacccgcc ggccggtggt
     2341 gtggccgcaa cgcttgggga actcctggtc ctcgatctgc accgcggcgg cgcccgcggc
     2401 ctcgtaggtg cgcacggtgt ggcggacgtt gagcgcgccg ccatagccgg tatcggcgtc
     2461 ggcgatcagc ggcgcgccgg tgccgtcggc gatgcggcgg acccgctcgg ccatgtcggt
     2521 cagcgtcgca aagccggcgt cgggcaggcc cagatgcgag gccgagacgc cgtagccggt
     2581 catgtacagc gcatcgaagc cgaggccgtc ggcgatgcgc gccgagatca tgtcgaacac
     2641 cccgggggcg acgatcaccg gcgcctcgcc gagccggcgc ctgagccgct tgcgtttctc
     2701 gcctgccgac atgcgcgcct ccctgtgcag cgccgacagc atgttgcccg ccgcgcacgc
     2761 ctgtcaaacc ttgcccgccg ggcgcgggat gcggatagtc ggccgatgac ccgtcccgag
     2821 acgccctatc cgcgcctgtt cagcccgctc gacctcgccg ggcggacctt gcgcaaccgc
     2881 atcgtccacc tggcgacggt gaccaacttc aacgacgcgg ggcggatcac cgaccgcttc
     2941 atcgcctatc acgccgcgcg cgccaagggc ggcgccggca tgatcgtgac cgagggcctg
     3001 tcggtgcacc cgagctcggc gcccaaccca cgcgtcgtcg cgctgtacga tcccgccaac
     3061 cgcgacggct tgaagaggct cgccggggcg gtcgagggcg cgggctgccg gctgctcggc
     3121 cagctctggc atgtcggccg ccagcagctg tgggggccgc aggcgacgcc gctcggcgtc
     3181 tcgcaccttc ccgacgcgct cagctggcag gtgccccacg tcatggaccg cagccagatc
     3241 gaggcgctgg tggcggcgtt cgtcgcctgc gccaggctgt tgcaggaggc gggcttctcc
     3301 ggcgccgagc tgcacggcgc gcacggctac ctgatcaccc agttcctgtc gccgtggagc
     3361 aacctgcgcg acgacgacta cggcggcgac cgcgtgcgcc ggatgcggtt cctcagcgag
     3421 gtgatcgcgg gcatccgcgc ggcgtgcggt gcgcgcttca tcctcggcct caagatgccg
     3481 gccgaagagg gagtcgcggg cggcatcgac ctcgccgagg cgcgcgcgat cactacggcg
     3541 ctcgcggccg acggcggcct cgactacatc gcctacagcc agggcaactt cagcctgagc
     3601 ctcgagcggc atttgcccga catgcatttc ccgtcggcgc cctatgcggc gctcgcgcgc
     3661 gcgctcaagc ccgcggcggg agcgatcaag gtgatggcgc tggcgcgaat cgcctcggcg
     3721 ggcgaggccg aggcgctgct cgccgacggc gccggcgagc tggtcggcct cggccggccg
     3781 ctgatcgccg agcccgcatg gcccgacaag ctcgcccggg gcgacggcgc ggagctgcgc
     3841 ccgtgcacct attgcaacta ttgctggagc gaggtgtatg ccgggcgcgc gctcgcctgc
     3901 gcccagaacg tccgcctcgg cggcacgcag gagctcgcgc cgtggatcgc cccggcgaag
     3961 tggcggcggg tggcggtggt cggcgccggc ccggccgggc tcgaggcggc atggctcgcc
     4021 gccagcgccg gccatcacgt gacgctgttc ggcgcctccg aggcgcccgg cggcaaggtg
     4081 cgaatcgagg cgctcttgcc cggccgtggc gaggcgatgg aggtggtccg gttccaggcc
     4141 gagcggatcg cgcgcttggg cgtcgagcag cgcctcggcc gcgcggcgag cgctgacgac
     4201 atcgccgccg ccgcgcccga ggtggcggtg ctggcgacgg gcgcgaccat gcagccgccg
     4261 ccgctggcgg gcggcagcgc cgcgatggcc gcgctgaccg atgccgtccc cggtctggtc
     4321 gcggacgcgg cgccgcgcgc gggcaccgcg gtgctgttcg accacgacca gtcgccgccg
     4381 gtctacgccg ccgccgagct gctggcggcg cgctacgccc gggtggtgat cgtcaccccg
     4441 gcgacccaga tcgcgcgcca cgtgccctat gtcagcgcga tcggcgtcca ccgccgcctg
     4501 ttcgccgccg gcgtcgagat cgtcatcggc tgccggccgg tggcgtggac cgacggccgg
     4561 ctggtgctcg agaacctgtt caccggcgcc gagaccgcga tcgcggacgt cgcctgcgcg
     4621 ctctatgcga cgccgcggcg ggccaatgac gcgctggccg caccactggc ggaggcgggc
     4681 atcgcgctgc acctgatcgg cgacgcctac gccccgcgca cgctgcatgc cgccatgctc
     4741 gaggcgcgcc gcgctgccga gaagatcgga agctcctgaa attattgctg tagcaaatgc
     4801 cgcggacctc atccttcgag acgcccgctg cgcgggctcc tcaggatgag gtttggtgta
     4861 gatggagcct catcctgagg agggccgccc ttgcttcgcc cgccgacccg tcctccgcag
     4921 cagcggcagc gccgctgccg atgcggaggg tggaagcggg cttcgcgaag gcgggaggcc
     4981 cgtctcgaag gatgaggctc gcctgcgaac gttgcgaatc cggacgccta cgctccgtcg
     5041 agcgcggcga cgaggcgggc ggcgtgctcg cgcttggccg ggtcggggtc gcgctggatg
     5101 atgcgcagca gggtcgcgcg cgcgaccggg tcgccgttgg cagcgatctc gaacgccatc
     5161 tggtcgcggc gctcgtaata ctgccagtcg aacggcgcga gcgagagcgg ccggcggcgc
     5221 cagtagtatt ggaactcgct cgatcgccag ccccggtaga tctggtccca gctgccggtg
     5281 ccggcgtagg gcgcgagcga ccgttccgcg accggctcgc cgagccgctg gtagcgcgcg
     5341 tcgaccgact ggcgcaggcg ggaggtcatg ttgggcagcg cgcggtggac ggtcatcgag
     5401 tggaagatca ccgcgtcgcc ttgggcgaag tcgcccgaca cccagcggcc gtcgagcggg
     5461 tcgcacacct ccatcgcgcc ggcgccgctg gagacgcgga actcgtgcac cccttgcgcg
     5521 tgcgagccct cggcgacggc gagcccgccc atggcgagcg ggcagtcgct caacggcagc
     5581 cacacggcga aggtctccgg cgtgccctgg atgtgcggga agtcctggtg cgcgggagtg
     5641 gtcagctccg ggcgctgcgg gaagatgttg cgcgggatca gcatcgggtg caccagcacc
     5701 tggtcggcga acatgcgcgc gaacaggccg agggtggccg ggtggtgggt cagcgcgtgc
     5761 agcgcctcga tccggtagat gcgcttcagc accggcacgt aacggtcgtc ggggtcggcg
     5821 caggcggcgt ccggattggc gatcgcgtcc gcgcgcgcat cttccgcgcc gagccagccg
     5881 gcttccgcga ccacgtcgag cacctggctg cgcacggcgg cgatgtccgc ggccggcacc
     5941 agcgcaggga tgaacaggta gccgtcctgc gccatgcgcc gggccagcgc cggcccgtcc
     6001 gcgacgatgt ccgaggaatc gacgaaccgc ctcactcgct ccacgtcggg cttactcctt
     6061 gatcgcgccg gtcatggacg aaacatagtg ctcgacgaag aacgcgtaca ggatcgcgat
     6121 cggcagcgag ccgaacagcg cgcccgccat cagcgagccc cagtggtaga cgtcgccctc
     6181 gaccagctcg gtgaccacgc ccaccggcac cgtcttgacc tcggacgagg agatgaaggt
     6241 gagcgcgtag atgaactcgt tccacgacag ggtgaaggcg aagatgccgg ccgagatcag
     6301 ccctggcacc gccagcggca ggatgatgcg caccagcacc tgcaggcgcg aggcgccatc
     6361 gaccagcgcg cactcctcga gctcgtaggg gatggtcttg aagtagccca tcaacagcca
     6421 ggtgctgaac gggatcagga tggtcggata ggtgaacatc agcgcgagcc gggtgtcgaa
     6481 cacgccgagc tggaccacca tcagcgacag cggaatgaac aggatcgagg gcggcaccag
     6541 gtaggcgagg aagatggcga caccgaccag gttgccgccg cgaaagcgca gccgggtaat
     6601 cgcgtatgcg gcgaggacgc tggcgaagat cgagaacgcg gtcgccacca ccgagaccag
     6661 gatcgtgttc cacaaccagg ccggatagga ggtctcgaac aacagcttgt tgatgtgggc
     6721 cagcgtcggg ctcgacaccc agaacgggtt gccggcctca aagtccaaga gctcctggtt
     6781 cgacttcacc gaggtgatcg ccatccagta gaacgggaac aggagcacga tcacgaacac
     6841 cgccagcggc acgtagatgg tgaccacgcg cctgggcagg gtggacaggt actccatgcc
     6901 ctcgcgcccg gcgcgcgcgc cgcgcctggt cattggcacg ctagtcattg gtgccctgct
     6961 gccagcgccg gcgctggaga ccgaaatagc tgatcatgat gcaggcgagc aggaacggga
     7021 tcatcgcggt cgagatcgcc gcgccctcgc cgagctggcc gccggggatg gcgcgctgga
     7081 acgacaaggt cgccatcagg tgggtggcgt tcaacggccc gccgcgggtc agcacgtaga
     7141 tcagctggaa gtcggtgaag gtgaacagca ccgagaacgt catcaccacc gcgatgatcg
     7201 gggtgagctg cggcagggtg acgtagcgga atcgctgcca ggagccggcg ccgtcgatga
     7261 ccgcggcctc gtacagcgac ggcgagatgg tttgcagccc cgcaagcagg gtgatggcga
     7321 cgaacgggat gccgcgccaa atattggcgg cgatcaccga ggcgcgggcg ttgttcggat
     7381 cgccgaggaa gttgatgtac cggtcgatca ggccgagctt ctcgagcgag tagctgatga
     7441 tcgagaactg ggcgtcgtag agccaccaga acgcgatcgc ggacagcacc gtcggcacga
     7501 tccagggcag cagcacgatc gcgcgcagga acgccttgaa cgggatacga ttgttcagga
     7561 tcagcgccag ccacaggccg agcgcgaact tgaagacgct cgccaccgtc gtgtacagga
     7621 tggtgttcca caccgacagc cagaacaccg ggtcgtcggc caggaacacg aagttctcga
     7681 cgccgatgaa gatgcccggc cggccgatgg tggagtcggt gaatcccagc cagattccca
     7741 agcccagcgg ataggcgagg aagcccacca gcaccgccgc cgccggcaac atgcacagga
     7801 agctcaacag cgccttgctg tcgcgcagcc gcgcgagccg gcgcccgagc ccgataacgt
     7861 cgggcccggg cgcggcaagc tcctgggcgc ggtcgatcat gcgccggtca tgtcctgcgg
     7921 taatagcgct cggcgcggcg ctgggcgcgc tgggccgcct ccttgggcgt gcgctggccg
     7981 gcgaccacct cggcgaacat gtcgaccagg acgaagtcgg cggcggtggc ggcggaggcg
     8041 tagccggagg agccggcaaa accgtcccac agcgcgcgct tgaggttgtc gcggaagggc
     8101 gtgtgcttgg gatcctcggt ccacaccggg ttgctctcat aggccgcgag cggctgggta
     8161 atgtagccga tcgaggcctg ctgccacggg tcgtactggt ccttctccat caggaagcgc
     8221 aggtattcct tggccgcgtt cggatacggg ctgtaggaga agatcatcgc caaggtgacc
     8281 agcgcgccct gggttggcct gcccaccggg ccgaccggca ggttggcgtg ctcgatgtcc
     8341 tcggcgatcg ccttgagctt cgcttcctcc gagttcttgg ccgcgtagta gatcgagatg
     8401 ccgttgaagg taagcccgat ctgctctgac aggaacgcct tgttgttgtg cggatcgagc
     8461 cacgagatgg tgcccgggat cagggtggcg tagagctcct tggcgtattc gagcgcggcc
     8521 agggtttccg ggcgctcgag caccacctgg tcgttctcgt ccacgatcat gccgccgtgc
     8581 gaccacagca accattggca cacggcgttg ccgtcgccga ccgcgtggcc gaaggcgaag
     8641 ccgggcgggt ggcccttggc ggcgagcgct tggcaggcgg cgaggaagcc ggcgttgtcg
     8701 ccgggcagcg cctcgaagcc ggcctcggcg agccacgagc gccggtagac cgtgcacgag
     8761 gtcggcccgc ccatcggcag cgcgatccag cgctcgccct tggtgccgta tgtgcggcac
     8821 acgtcgtacc agccgccata cttggcgccc aggtagtcgg cgaggtcgtt gacgtcgatc
     8881 agcttgtccg ggaactggtg agggtcgtcg ttgaagccga ggatgatgtc cggccccttg
     8941 ccgacattgg cggcgaccgc agccttgggg cgcaggtcct cccaggactc gttgtcgacg
     9001 cgcaccggca ctccggtgat ctcggtgaag cgggcggtat tggccgtgaa cacggtctcg
     9061 tcgccctcga cgaagcgctt ccagcgcagc acgcgcagct cggcgcccgc ctcgggcgtg
     9121 aatttgagct cttgcgcgcg cgccgcgcgc cccagcggcc caaggccgag cgcgccggcg
     9181 gcggcaagtc ccgcgccgcc cgtcagcacc cctctgcggg tgattgtcgc catggttgac
     9241 cttcctttcg ttgcggtccc ctccgcattg gccgcgggcg cggcaggcgc ggcgctacag
     9301 gtgcccgccg gtggccgcgt cgaacaggtg cacggtgccc gcacgcgggc tcaggtggat
     9361 cacctggccc ggcttgaact ggtggcgctc gtcgaacacg gcgcagatgt cgatgccggc
     9421 catgcgcgcg aacaccaggg tgtcggcgcc ggtcggctcg accaccgaga ccttgcaggc
     9481 gatgccgccc tcgccgtcgg caagctcgag gtgctccggg cggacgccga acacgaccgc
     9541 gccgtcggcg cgctccggcg ccggcgcctg aagcggcagc gcggtgccgt cggcggcctc
     9601 gacccaggcg cgcgcgccgc cgtcgcggcg cagccggccg gtgatgaagt tcatcgccgg
     9661 cgagccgatg aacgcggcga cgaacaggtt gtcggggcgg tcgtagatcg ccagcggctc
     9721 gccgacctgc tcgacccggc cgtcgttgag cacgacgatc cggtcggcca tggtcatcgc
     9781 ctcgacctgg tcgtgggtga cgtagaccga ggtggtgcgc aggcgctggt gcaacgcctt
     9841 gatctcggtg cgcatctgga cgcgcagctt ggcatcgagg ttggacagcg gctcgtcgaa
     9901 caggaacacc gccgggtcgc gcacgatcgc gcgccccatg gcgacgcgct ggcgctggcc
     9961 gccggaaagc tggcgcggat agcggtcgag gtactcctcg aggccgagga tgtcggcggc
    10021 gcggcgcacc tggcgctcga tctcgccccg gtcgacgcgc gcgagcttga ggctgaacgc
    10081 catgttcccg tagacggtca tgtgcgggta gagcgcgtag ttctggaaca ccatcgccac
    10141 gttgcgctct ttcggcggca tcgcgttgac cacccggcgg tcgatcagga tctcgccgcc
    10201 ggtaacctcc tcgaggccgg cgaccatgcg caggaaggtc gacttgccgc agccggaggg
    10261 gccgacgagc accacgaact cctcgtcgcg gatatcgatg tcgattccgt gcaggacctc
    10321 ggtggcgccg aacatcttgc ggacgccgcg cgcttccacc gtcgccatgg ggaccgtccc
    10381 gtgatcgcag cggctgttgc ctcgccgatt atggcgcccc tgcccccgcc gtcacaaggg
    10441 cggccgcaag cgagctaccc ggccgcggtc agctgccgac ggtgagcggg ctgaccaggc
    10501 ggcgaagcag ggcgacgatg ctgagcgcgg tgatgcggcc ggtgcgcgga ttgtccgcgc
    10561 tgggcacgtt ctcgatggtc atcgagaagc gggcgctgtc ggcctcgacc tcgatgtggt
    10621 ggacgttgcg gctgagcgcc gggtcggccc acacctcgag gcgggtgcgc tcgggcccga
    10681 tgccggcgag gctcaaggcg gcggcgacgt tgacgttggc cggaaagccg cgcgcgccct
    10741 cgccggcgct gcccgcgaac accttcagcg gcgccgtgag gccgtcgagg ctgatgccgg
    10801 cctcggcgag atagggcgcg ccggcgagcg aggccggcgg cttgcgcgtg accatggtga
    10861 ccgcggtgat ctcgccctcg gcggcggcgc gcaccgcatc gaggccgagc agggcgccgg
    10921 tcggcacgac gatccgcgcg ccgctccgcc gggcaagctc gaccaggtcg gggtgggcga
    10981 gcagctggcc gacgctcgcc ggcaccaggg tgcgaccggc ggcgagcacg ggctcggcga
    11041 tggcgcggaa ggcggccgcc ggcgcgcatt cgaccacgac ctcggcgtgc tcggcgagcc
    11101 gcggcgctgc gcacacctcg ggcggggcgc ggaaatcggc gactcgcgcg cgggcgcggg
    11161 ccaggtcgcg cgccgccacc gcgaccagcg ccagcccgtc gatgccggcg tcgagccggc
    11221 gcgcgaccgc catgccgatg gcgccgaggc cggcaatgcc gacccgcagc gcaccagggc
    11281 tcgcctccac cattcccgtc cgccccccgc agcccggccg attgttggta ggttcgcgct
    11341 tacgctaagg tagccgcgca agaggaggca agctgcgatg cggacagccg gctgggcgat
    11401 ccgggtgctg gcggcggcgg tgctgtggac gggcgcgggc gccgcggggg cgctcgacat
    11461 gccgtgccgc acggcgcgcc tgatcgtgcc gtgggcgccg ggcggggaga ccgcgatcaa
    11521 cttcgagctg atcgccgatg cggtcaaccg aagcggcgtc tcgccgcgcc tgcaggtggt
    11581 cacgattccc ggccagggcg gcaacaaggg cgccaaggtc gcgcgcgacg ccaggcccga
    11641 cggctgcacc ctgttcgcgc tgcatcagag cgcgatcacc agctacctga gcgggcgggt
    11701 cgacttcacc tgggacgcgt tcgagccggt ggcgctgttg accggcacgc cgtcgatgat
    11761 cggcgcccgc ccgggcgcgt cctatgccga cctcgccggg ctgatcgccg ccgcccgcgc
    11821 cgcgccgcag accgtgcttg ccggcagcac cctcggctcg accagccact tcctgttcct
    11881 gctgctcgag aaggcggccg gcatcaagct caagcacgtc tcctacgacg gcacccgcga
    11941 gcggctgacc gcgctgctcg cccagaccat cgacgtgggc gagatcaacg tggtcagcgc
    12001 caaggagtac ctcgacgacg acgcgctcaa ggcgctcggc atcgccaccg cggcgcgcga
    12061 tccgctggcg cccgaggtgc cgacgctcgc cgagcagggc gtcgacatca ccttcggcac
    12121 cgaccgcggg gtggtcctgc ccaagggcgc ggcgggcgag gtcgtggcat tctgggagcg
    12181 cgccttcctc gacgccctcg ccgggcctga gctccggcgc gcgctcgagg ccaagggcaa
    12241 caccatcatc ggccagggcg ccgaggccta ccgcgccgcc ctcgccgccg agttcgccgc
    12301 gctcgaggcc gccgccgccg aggccggcct gtaccggcgc tagcgcgccc tccggtcgca
    12361 ttgacctgcg attccgccgc cgcgctcggg ctggtcgcgc tgtgcgcggc gctgatcgcg
    12421 gcgaccttcg acatccgcga gaccaactac gccacccccg gcgccgaggt gtggccgcgg
    12481 atcgtgctcg cctggctgtc ggcgctgacc ctggtctatc tggcgcgctc gctggtgcgc
    12541 gcccggcgcg cgcgcgggcc cgaggcggag ccaagaccgg gtctgcccgc caacgtcgcc
    12601 tggtgcttcg ccctgttcgc cgcgttcctg ctggcgctgc cctatgccgg catgctggtc
    12661 gccggcatcg gcttcgtgtt cgcggcgctg tgcgtgatgg gccggcgcga ccgccgcgcg
    12721 ctcgcccgtc acgccgcgat cgcggtggcc tctgtcggca cggtgtgggc ggcgttcacc
    12781 tacgcgctcg gcgtcatgct gccggcgggc gagctgctcg gggcatggta gcgggccggg
    12841 gcggacgctg atgcagggcg cggacatcct ggccgcggcg gcgagcgtgc tcgacccgct
    12901 caccctcgcg ctcctcgcca tcggcgtcgc cgccggcctg atcgccggcg cgatccccgg
    12961 cttcaccatc gccatggcgg tggtgctgac cttgcccttc accttcggca tggcgccgat
    13021 ccaggggctc gcgaccatga tcggggtgtt cgtcggcggc ctgtccggcg ggctgatggc
    13081 ggcgatcctg accggcatcc cgggcacgcc gtcggcggtc gcgaccacct tcgacggctt
    13141 cccgatggcg cgcggcggcc ggccggggct ggcgctcggc atcggcgtgt gggcgtcgtt
    13201 cgcgggcggc ctgatcagcg ccgtgatcct ggtgaccttg gccccggtgc tcgccaggat
    13261 cgggctcgag ttcggcccct gggactactt cgcgctgatc gccttcgcgc tcaccatcac
    13321 cgcgagcctg gccggcgagg acatcctcaa gggcctgatc gcgggcgcgc tcggcctgct
    13381 cgcggccacc gtcggcgagg acaacatcaa cggcgtcgcc cgcttcacct tcggcatcga
    13441 cgccctgcac cagggcttcg cctacctgcc ggtgctgatc gggctgttcg ccttcagcca
    13501 gctgatgagc gacgtcgccg atccggtgcg cgcgcggagc ccgctgctcg atgtgccgcg
    13561 ggccgcggtg cgcatccggc accgccaggc gatccgcacc gtggccggcg agtgggcgac
    13621 gctggtccgg tcctccctga tcggggtgtt caccggcatc ctgccggccg ccggcagctc
    13681 gatctcgaac atcctcgcct acgaccaggc caggaaggcg tcggcgcacc cggagcagtt
    13741 cggcaccggc attccggccg gaatcgtcgc ctccgagtcg gccaacaacg ccaccgccgg
    13801 cggcgcgctg atcaccatga tggcgctcgg catcccgggc gacatcgtca ccgcggtgat
    13861 gctgggggcg ctgttgatcc acgacgtggt gccgagcccg tcgttcatcg ccgatgcgcc
    13921 ggtgctggcc tacggcatct tcattgcctt cttcctcgcc catttcgtca tgatcgcgct
    13981 gcaggcggtg tgcctgcgcg gcttcctgct ggtcacgcgg gtgccgatgt acgtgctggc
    14041 ggcggtgatc ctggcctatt gcgcggtcgg cgtgttcgcg ctcaacaacg tcgatttcga
    14101 cctctggacc ttgctgctgt tcggcctcgc cggcaccggc ctgcgcgcgc tcggcttgcc
    14161 gctggcgccg atgatcctcg gcgtggtgct gggcaccacc gccgaggtca acctgtcgcg
    14221 ggcgctgacc atcagcgacg gcgccgccca agcggcggcc gacttcgtga cccggccgtg
    14281 gtcgctcttg ttcctgatcc tggcggcctt ctcggccgcc ttcccctggt accaggcggc
    14341 gcgcgcccac gcccggccct ggacccggct gtacctgccg gcgatggcgg tcgcggtctc
    14401 aggccccctg ttcctgatgg acggctggtt caggccgctg gtcggggccg gcctgctggt
    14461 ggccgggccg tggctcgtct ggcggcgcct ggtccgcgcc tgaagcccgg ccgcggggct
    14521 tgaacggcgg cgcagggcgg cgtattccgg ccgcgcacgg gtatggtaga ctcacgccga
    14581 ggcccggccc tgcgcggctt gctaccccgt tcgtcgaccg atccggaaag gacgtccgca
    14641 tggcctctgg aggattcgat ttctgcctcg gcatcggcgg cgccgccggt cagggcatcg
    14701 ccacgcccgg caacgtgctg gcccgcatct tcgcccgccg cggcatccac ctgagcgcgt
    14761 acaacgccta ccagtcgatc atccgcggcg gccacatcct gttgaccatg cgcgccagcg
    14821 ccgagccccg gcacagccac ggcgaccggc tggacctgct gatctgcctc aaccaggaca
    14881 ccatggaccg ccacctcggc ctgatgggca agggcgcgcg ggtggtgttc aacgccgatg
    14941 ccatcaagcc cggcaaggcc gcgagcgggg tccagatgtg cccgctgccg gtcgccgagc
    15001 tgtccgggaa cagccgcaac aagctggtcc agaacgtcgc ctcgctgggt gcggtggcga
    15061 gcctgctggg cctgagcttc gacatcctcg aggcggcgct cagccagcag ttcggcggca
    15121 agggcggcgg tgcggtcgac gagaacgtgg ccgtcgccag ggccgcgttc gatcacgccg
    15181 cggccagctt cgagccgctg gccgagccga tccccgaggg caccaggccg ctcggggtgt
    15241 ggaccggcaa cgacgcgctg gccatgggcg gcgccgccgc cggggtcaag ttctactgcg
    15301 cctacccgat gagcccgtcg accggcgtgc tccactggat ggcccgccac gcccgcgacc
    15361 tcggcatcat ggtccgccag ggcgaggacg agatcgcggt cgccaacatg gtgatcggcg
    15421 ccgcccaggt cggctgccgg gcgatgtgcg cgacctcggg cggcggcttc gcgctgatga
    15481 ccgaggcgat cggcagcgcc gggatgatgg agatcccggc ggtgttcatc aacgtccagc
    15541 gcgccggccc gtccaccggg gtgccgacca agaccgagca gggcgatttg tggcaggcgc
    15601 tgggcgcgag ccagggcgac ttccagcgct tcatcgtcgc gccgaccgac gcgctcgacg
    15661 gcttcaacac cctgcccgag ctgttcaacc tgtgcgaccg ccaccagtgc ccggggatcc
    15721 tgatctcgga cctgctgatc tcggagggca cgttcacggt cgatcccgag gcgctcgacc
    15781 tgcagcccgc gatcgagcgc ggcgaggtga tcaccgaggg ccgcgtcgac ggcgcctatc
    15841 gccgctatct cgacaccgac agcggcatct cgccgcgcgc gctgcccggg gtcgagggct
    15901 acgtgcacgt ggtcgcgacc gatgagcacg accaggacgg ggtgctgatc agcgacgagt
    15961 tcaccaaccc gatcaagcgc cgccagatgg tcgagaagcg ggcgcgcaag ttcgacggcg
    16021 tggccgagcg gatcgcgccg ccggcgctga tcgggcccgc ggacgcggac gtcaccctgg
    16081 tcggctgggg ctcgaccaag ggcgtgatcc gggacgcgat ggcggcgctc gccgacgccg
    16141 gcatctccgc caaccagctg ccggtgaaat ggatcgtgcc gttccacgcc gccgaggtcg
    16201 gccgcatcct ggccgaggcc aagcgcgtgg tggtggtcga gaacaaccat tccgggcagt
    16261 tctaccgcta cctgcgcagc gagaccggcc tcgccgtcga cgggcacatc cgcaagtacg
    16321 atggcgagcc gttcatgccg caccatatcg tcgagggcgt gcaggcgctg ctcgccggcg
    16381 agaccgaccg ctacgtgccc gagcacgaga tcatggtttg agggtgagca aatggcaggc
    16441 gtagcgatcg aggcggtcga ggccgagcgg ctgacggcca aggacttcaa gggcaaggtc
    16501 gatcccgact ggtgcaaggg ctgcggcgac ttcggggtgc tcaacgcgct ccagcgcgcc
    16561 tgcgccgagc tcggcctcaa gccgcacgag atcctggtgg tcagcggcat cggctgctcg
    16621 tcgaacctgc ccgggtactt caacgcctac ggcatgcaca cgctgcacgg gcgcacgctg
    16681 gcggtggcga ccggtgccaa gctcgccaat cacgcactga ccgtgatcgc gaccggcggc
    16741 gacggcgacg gcttcggcat cggcggcaac cacttcaccc acaccgcccg gcgcaacgtc
    16801 gacctcacct atatcgtcat ggacaaccag atctacggcc tgaccaccgg ccaggtgtcg
    16861 ccgaccagca tgcgcaagat gcgcaccaag agcaccccgt tcggcagcgt cgaggggccg
    16921 ctcaacccga tcacgtcggc gatcatgaac ggcgccacct tcgtcgcccg cggcttcagc
    16981 ggcgatccga aatacttaag cgacatgatc atgcgcgcga tccgccaccg cgggttcgcg
    17041 ttcatcgacg tgttcagccc gtgcgtcacc ttcaaccacg acaacgactt cgcgttcttc
    17101 aaggagcggg tgaagcggct cgacgacgac ggccacgaca gaagcgactg gaaggcggcg
    17161 tgcgagaagg cgatggtctg ggacgacgtc atctataccg gggtgttcct tgagcgcacc
    17221 gacgagccct cgctcggcga gcaggagccg gtgctcgccg ccggcccgct cagcagccgc
    17281 ccgccgggcc tgaccaagga gcaggcgaac ggcatcatcg cgcgcatgat gtgagggcgc
    17341 ggcgcgcgcg ccctatttga tcagctcgcc caggccccag accggtaccg cccggccgcc
    17401 gacggtcagc tcgccggtga ccagcgtcat ggcgaagccg ccgatcagcc agttgccgaa
    17461 gctccgccgg tcgcgcgcga ccagagtgag ccgcgccgcc ggcgcatcgg gccagccgcc
    17521 cgaccaggcg ctcgcccaac ggtagacgcc gagcgcctgg tcgctgtcct cgaccgtgag
    17581 ctcggcgtcg gcgaggcgtc gcgcacgccc gttgaggacc gcgaagccgt cgacctcgcg
    17641 cctgccgtcc gcgtcctggc tttgcaggta cacgtcgtcc ccgccggcgc cgaccgtctc
    17701 gccggcgacc aggtagaagc tctgccagtc gttccaggtg ctccaatagg tgcgcgcgag
    17761 ccggttccag ccgtcgaagc ggacgaactc gtagatcacc cgcccgtcga gcctgcgccc
    17821 ctgccaggcg agcgtcgccg cggccgagcc gagccggcgg gcgcgcgcgc cggtcgagtc
    17881 gctcatgcga atctcgatcg gccgggtggt caaggtgagc gcattcggct cgctcaggat
    17941 ggtcaccccg tccgcgggcg tgcccgagta ccggaagtcc gccgactcgg gcacgtcgtc
    18001 gagcacgcgc gcggcgttgg cataggggcc ggagccggcg aggtccaccc agcctgccgc
    18061 gtcgtcgtag agcgcggcga acagctcggc ctggaagtcg tcgccgtcgc gcccgcggtt
    18121 ggcatccagc gcgaacgcga cccggcccgc cgcatcggcg ccgacgaacg agtagtagtc
    18181 ggagacatag gcgaggtcgt cctcgcccgc gccgccggcc cggcagccgg cggccaccag
    18241 cagcgcgacg gcaaatgccg atcgccttgc ccgcgtcatc gccggcgcgc tcagaactgg
    18301 acgcgctggg tgaggcagcc gtcgacgatc aggttggtgc cggtgacgaa gctcgccgcg
    18361 gggctggcca ggaacgcggc cgagcgcgcg acctcgtccg gggtgcccat gcgccccatc
    18421 gggttgcggg cgagcgcgcg cttgaacagc tccgggctct gctgctcgcg ctggtgccag
    18481 acgccgccct tgaagtagat ggtgccgggc gagaccgagt tggcgcgcac gcctttcgcc
    18541 gcgagctcgt gggcgaggtt ggagacgtag gtgatcagcg ccgccttcaa ggcgttatag
    18601 ggacgcacgc cgccgaacgc ctcgagggcg gcgaccgacg agatggcgac gatggcgccg
    18661 gcgtgcgagc gctcgagata cggcagcgcg gcctcaaccg cgttgaaggt gccgaggatg
    18721 tcgccgtcga acgcctgccg ccagccggcc tcgtcgaggc tggtgccgaa gcctgagaca
    18781 ttggcgacca ggatgtcgat gccgccgagc tcggccgcgg ccgcgtcgca ccatgccttg
    18841 agcgccgccg catcgcgcac gtcggccgcg ccgccggtgg cgttgacgcc cttggccttg
    18901 agcgcggcga ccgcgggcgc gatcgcgccc ccgccgcgcg cgcagatgcc gacgtggcag
    18961 ccctcgcccg ccagcagctc ggcgatcgcc cggccgatgc cgcgcgtgcc gccggtgacc
    19021 accgccttca agccgcccaa gcccagatcc atcgcctccc ctccccggtt cgcgccgcgg
    19081 ccgagcctag cggcatatgg cggtcagaac caagtggcct tgtcgaccac gttgcggagc
    19141 gcccgcccct ggtcgaagcg gcggcagttg tcgagga
//
LOCUS       DASWHU010000363         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2835773, whole genome shotgun sequence.
ACCESSION   DASWHU010000363 DASWHU010000000
VERSION     DASWHU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835773"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VGB88_13570"
     CDS             <1..514
                     /locus_tag="VGB88_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648528.1"
                     /translation="AGNGKMKLVDRWNYHNELGAQVPLQALRVMYAASGKQPAACLLC
                     DQRPVIEHGLYWMTPSTLDEGRYLTAILNSETARARGERYQARGQFDVRHFDKVPFNQ
                     PIPRFDGGNELHVALAEAAGRAEKIAAKVELPKGVKFQRARGLVRAALAEARISQRID
                     DLVAELLDAV"
     gene            592..1704
                     /locus_tag="VGB88_13575"
     CDS             592..1704
                     /locus_tag="VGB88_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX9648529.1"
                     /translation="MASARTVALALAALAALAAGRPAAAEIRLDLIRLPAGFAIEVYA
                     EGVANARAMALGARGTVFVGSRAAGNVYALVDEDGDQRADKGWLIDSGLDMPSGIAFH
                     DGALYVGAVSRILRYDDIEARLADPPPPAVVTDRLPDSSHHGWKYLAFGPDGKLYVPV
                     GAPCNVCDPDSPFASMLRMNADGSGAEVFARGIRNSVGFDFHPETGELWFTDNGRDML
                     GDDRPPCELNRARAPGLHFGFPFCHGGVIQDPEFGRGRSCAEFEPPAQPLGAHVAPLG
                     MKFYTGTMFPPAYRGQVFIAEHGSWNRSRKIGYRVSLVRIDPAGAATAYEPFAEGWLQ
                     GQDNWGRPADVLVMPDGALLVSDDQAGAIYRISFGS"
     gene            complement(2046..2294)
                     /locus_tag="VGB88_13580"
     CDS             complement(2046..2294)
                     /locus_tag="VGB88_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648530.1"
                     /translation="MSGSAAFRRAGGAASVAVAPASIADSSSSTGPTGGASARSSSAA
                     TAAAACAGAARADALTSGARIACWSMRWWLMKSWSPGR"
     gene            2303..>3371
                     /locus_tag="VGB88_13585"
     CDS             2303..>3371
                     /locus_tag="VGB88_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9648531.1"
                     /translation="MAAARTLIAGARRPALVAGIQTRARGAAEVARLADALACPVLTT
                     YKAKGVYPDGGARHAGLFTGARTEAQWLADCDLLILYGVDPIELVASPWPYKMAVLEL
                     AEVEIRPHYTTPKASVFGPLPRLAEAVVASARAANWPTSPRPQALPEPALDGLSPHAV
                     ARALARAAPAGARLAVDAGAHMLPAMAHWPARAPFGVLISNGISTMGLALPAAIASAL
                     EEPERPVVVMTGDGGLAMCLAELATCARERLGVTVVVFNDGALKLIDVKQQQRGFATR
                     GVRYPPLDFARIAEGAGMRGVRVETEAALEAAIEVAFDGLPARSRSGVASAKAGGGAT
                     LIDARIDAGGYRAMFEAIRGAP"
BASE COUNT          455 a         1242 c         1210 g          464 t
ORIGIN      
        1 ggctggcaac ggcaagatga agctagtaga caggtggaat tatcacaacg agttgggcgc
       61 gcaggtcccc ctccaggcct tgcgcgtcat gtatgccgcg tctggaaaac agcctgccgc
      121 atgcttgctc tgcgatcagc gccctgtgat agagcatggc ctatactgga tgacgccctc
      181 gacgctggat gagggaaggt atctgaccgc catcctcaac agcgagacgg cgcgagcgcg
      241 gggagagcgg tatcaggctc gcggccaatt cgacgtgcgt catttcgaca aagtcccgtt
      301 caaccagccg atcccgcgct tcgacggtgg aaacgaactc catgtcgccc ttgccgaggc
      361 ggcggggcgc gccgagaaga tcgccgccaa agtggagctt cccaagggag tcaaattcca
      421 gcgcgcccgt ggtctcgtcc gcgccgcgct ggcggaggcg aggatctcgc agcgcatcga
      481 cgatctcgtg gcggagctgc tcgatgccgt atgatcggcg ccggcaaacg attggcaatg
      541 cccgggccgc tgtgctgtaa agtcgggatg caaaaggaac ggggaatcct catggccagc
      601 gcccgcaccg tcgccctcgc cctcgccgcg ctcgcggccc tcgccgcggg gcgtccggcc
      661 gcggccgaga tcaggctcga cctgatccgc ctgcccgcgg gcttcgcgat cgaggtctac
      721 gccgagggcg tggccaatgc gcgcgcgatg gcgctgggcg cgcgcggcac ggtgttcgtc
      781 ggctcgcgcg cggcgggcaa cgtctacgcg ctggtcgatg aggacggcga ccagcgcgcc
      841 gataagggct ggctgatcga cagcggcctc gacatgccga gcggcatcgc cttccacgac
      901 ggcgcgctct atgtcggcgc ggtcagccgc atcctgcgct acgacgacat cgaggcccgc
      961 ctcgccgacc cgccgccccc ggcggtggtc accgaccgcc tgccggacag ctcgcaccac
     1021 ggctggaagt atctcgcctt cggccccgac ggaaagctgt acgtgccggt cggcgcgccg
     1081 tgcaacgtct gcgatccgga ttcgccgttc gccagcatgc tgcgcatgaa cgcggacggc
     1141 agcggcgccg aggtcttcgc ccgcggcatc cgcaactcgg tcggcttcga ctttcaccct
     1201 gagaccggcg agctgtggtt caccgacaac ggccgcgaca tgctgggcga cgaccgcccg
     1261 ccgtgcgagc tcaaccgggc gcgcgcgccg ggcctccatt tcggattccc gttctgccac
     1321 ggcggcgtga tccaggaccc ggagttcggc cgcgggcgct cctgcgccga gttcgagccg
     1381 ccggcgcagc cgctcggggc ccatgtcgcg ccgctgggca tgaagttcta cactggcacc
     1441 atgttcccgc ccgcctatcg cggccaggtg ttcatcgccg agcacggctc gtggaaccgc
     1501 agccgcaaga tcggctaccg ggtgagcctg gtgcgcatcg atccggccgg cgctgcgacc
     1561 gcctacgagc cgttcgccga gggctggctg caaggccagg acaattgggg ccggcccgcc
     1621 gacgtgctgg tcatgcccga cggcgcgctg ctggtctcgg acgaccaggc cggcgccatc
     1681 tatcgcattt ctttcgggag ctgacgcgcg atgcccggtc ccgacgcgac tcggtcggcg
     1741 acggtgagcg ccaccgtcgc cgacgcgatc gcggcaacgc tcgcgtccta cggcgtgcgc
     1801 cggctgttcg gcgtgcccgg cggcggctcg agcctcgagc tgatcgaggc ctgcgcgcgc
     1861 cgcgacatcc gctacgtgct cacccacacc gagtgcgcgg cggcgatcat ggccgcggtc
     1921 acaggcgagc tgaccggcac ccccggcgtg gtctcggtcg gcctcggccc gggcgcggcg
     1981 agcgtcgtca acggcgtcgc ctatgccgcg ctcgaccgcg cgccggtgct ggtggtggcc
     2041 gacagctacc gccccggcga ccacgacttc atcagccacc agcgcatcga ccagcaggcg
     2101 atcctggcgc cgctggtcaa ggcgtcggcg cgcgccgcac ccgcgcacgc cgcggccgcg
     2161 gtggcggcgc tgctcgagcg ggcgctggcg ccgccggtcg ggccggtgct gctcgagctg
     2221 tcggcgatcg acgccggcgc gaccgcgacc gacgcggcgc cgccggcgcg ccggaaggcg
     2281 gccgagccgg acatccggcg acgtggcggc ggcgcggacg ctgatcgcag gcgcgcgccg
     2341 cccggccctc gtcgccggca tccagacccg cgcgcgcggc gcggccgagg tcgcccggct
     2401 cgccgacgcg ctcgcctgcc cggtgctgac cacctacaag gccaaggggg tctacccgga
     2461 cggcggcgcg cgccacgccg gcctgttcac cggcgcgcgc accgaggcgc agtggctcgc
     2521 cgactgcgat ctcttgatcc tgtacggcgt cgacccgatc gagctggtcg cctcgccgtg
     2581 gccctacaag atggcggtgc tcgagctcgc cgaggtcgag atcaggcccc actacacgac
     2641 gcccaaggca tccgtgttcg ggccgctgcc gcggctcgcc gaggcggtgg tcgcgagcgc
     2701 gcgcgcggcc aactggccga cctcgcccag gccgcaggcg ctgcccgagc cggcgctcga
     2761 cggcctgtcg ccgcacgcgg tggcgcgcgc gctcgcccgc gccgcgcccg cgggtgcgcg
     2821 gcttgcggtc gatgccggcg cccacatgct gcccgcgatg gcccattggc cggcgcgggc
     2881 gccgttcggc gtgctgatct cgaacggcat ctcgaccatg ggcctcgcgc tgcccgcagc
     2941 catcgcctcc gcgctcgaag agcctgagcg gccggtggtg gtgatgaccg gcgacggcgg
     3001 gctcgccatg tgcctggccg agctcgccac ctgcgcgcgc gagcgcctgg gcgtgaccgt
     3061 ggtggtgttc aacgacggcg cgctcaagct gatcgacgtc aagcagcaac agcgcgggtt
     3121 cgcgacccgc ggcgtgcgct acccgccgct cgacttcgcc cgcatcgccg agggcgcggg
     3181 catgcgcggc gtgcgagtcg aaaccgaggc cgcgctggag gcggcgatcg aggtcgcctt
     3241 cgacggcctg cctgcgcgaa gccgaagcgg cgtcgcttcg gcgaaggcag gcggcggcgc
     3301 gaccctgatc gacgcgcgca tcgacgccgg cggctaccgc gcgatgttcg aggcgatccg
     3361 cggcgcaccc g
//
LOCUS       DASWHU010000364         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_237424, whole genome shotgun sequence.
ACCESSION   DASWHU010000364 DASWHU010000000
VERSION     DASWHU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237424"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VGB88_13590"
     CDS             <1..1290
                     /locus_tag="VGB88_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9648532.1"
                     /translation="QSFGEGDKPILTASIVMQFEAGIADNDKPNGQDVIGTTNGKRDG
                     KFVGGRFAEIWFQGELTADNGLVYGARISLAADTPTRTNTYPGRKYIWLSGGWGSIEL
                     GDWIGPASGLAINPMAQEVSGNGLFDHAATEYIKIPNSGLTPPGDFQVFNGNYLGWFV
                     ADFGTKVSYYSPKIAGFQAGASYSPTTDNIGQSLSNRGGTAQALNNNGGFSDAVGLGV
                     RYDGDFSGLTFGAAWTAVYGNADDTTSCSALAGGATTGTCTGIQHFKRDDLATWNIGA
                     KVGYAGFILAGNYYDDDGSGFREIEQAQVNGWTVVGTYETGPIKIGLNYMTMEVEHNA
                     LWAARTGFGTAGTIAAPIGARNAINTFALPFTAGVTGDRTVVDSYNAEGFGGGVGYQL
                     APGLKLYADIGYFDFDAPIPELENNGIVGYGGIYVSF"
     gene            1419..1982
                     /locus_tag="VGB88_13595"
     CDS             1419..1982
                     /locus_tag="VGB88_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEX9648533.1"
                     /translation="MRHLCLSVALIGLAIATAGCGDTDVPVEATYPSDNPRPPGTLLG
                     KNQQTYYEGNSLFGGDGIVLFGGPNYEPDRSDGAGIGVNSFLWRASLDTLSFMPLVSA
                     DPFGGVIITDWYTPPQAPDERFKVSAFILDRSLRADGVRVSVFRQTQQSPGNWVDAPL
                     AESMATDLENAILARARQMRIRTLASQ"
     gene            2275..4833
                     /gene="leuS"
                     /locus_tag="VGB88_13600"
     CDS             2275..4833
                     /gene="leuS"
                     /locus_tag="VGB88_13600"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9648534.1"
                     /translation="MSRYDPQSTEAKWQRAWHDAGAFRTGRHGDRPPYYVLEMFPYPS
                     GRIHMGHVRVYTLGDLLARYKRARGFDVLHPMGWDAFGMPAENAAMEEGVHPATWTWS
                     NIEVMRGQLKSMGLSYDWTRELATCSPDYYRHEQRMFLDLLAAGLAYRKQAWVNWDPV
                     DKTVLANEQVIEGRGWRSGAPVERRLLPQWLFRITAFADDLLDSLARLDRWPDSVRTM
                     QANWIGRSDGAVVRFRLAGREGRLEVFTTRPDTIFGASFVAIAPNHPLAAELARFDAA
                     LAGFIEDCNRIGTSEAALETAEKQGFRTALEALHPFRAGLRLPVYVANFVLMEYGSGA
                     IFGVPAHDQRDLEFARKYALPVRIVVRPRDAGEDFAIDGEAYAGDGVAVNSDFLDGLS
                     VADAKGAVTRRLAAAGDGEARTSYRLRDWIVSRQRYWGCPIPVIHCPGCGIVPVPAAD
                     LPVTLPEDVTFDEPGNPLDRHPSWKHVACPTCGSAAVRDTDTFDTFVDSSWYFARFAS
                     PHAELALEPQAMRRWLPVDQYVGGIEHAILHLLYSRFFMRALRQCGYIDFDEPFAGLL
                     AQGMVCHETYREGRSWLYPEQVERGADGSARRRDTGAAVTVGRSEKMSKSRRNVIDPA
                     DMIARYGADTARLFMLSDSPPERALEWTAAGIDGAFRYVQRLWRLVAEPPAALAPRGT
                     AMANRLTPAGLELRRAVHRTIAAVTDDIERFHFNKAVARVRELTNAVASFEVSAADAG
                     AVLREALETLVLLIGPMTPHLAEELWRALGHDRFLCDQPWPAADPALVAVETVVIAVQ
                     VNGKKRATVEVVAGSDAGTVEARALAEPNVASFIAGKPIRKVIVVPDRIVNVVV"
BASE COUNT          726 a         1753 c         1608 g          748 t
ORIGIN      
        1 cagtcctttg gcgaggggga caagccgatc ctcactgcct cgatcgtgat gcagttcgag
       61 gcgggcatcg cggacaacga caagcccaac ggccaggacg tcatcggcac caccaacggc
      121 aagcgcgacg gcaagttcgt cggcggccgg ttcgccgaga tctggttcca gggcgagctg
      181 accgcggaca acggcctggt ctacggcgcg cgcatctcgc tcgccgccga cacgccgacc
      241 cggaccaaca cctatccggg ccgcaagtac atctggctgt cgggcggctg gggcagcatc
      301 gagctgggcg actggatcgg ccccgcctcg ggtctggcga tcaacccgat ggcgcaggaa
      361 gtgtcgggca acggcctgtt cgaccatgcc gcgaccgaat acatcaagat cccgaacagc
      421 ggcctgacgc ctccgggcga cttccaggtg ttcaacggca actacctcgg ctggttcgtc
      481 gccgacttcg gcacaaaggt cagctactac tcgcccaaga tcgccggctt ccaggccggt
      541 gcctcgtact cgccgaccac cgacaacatc ggccagagcc tgtcgaaccg cggcggcacc
      601 gcccaggcac tcaacaacaa cggcggcttc tccgacgcgg tcggcctggg cgtgcgttat
      661 gacggggact tcagcggcct gaccttcggc gcggcgtgga ctgccgtcta cggcaatgcc
      721 gacgacacca catcgtgctc ggcgctggcg ggcggcgcga ccaccggcac ctgcaccggc
      781 attcagcatt tcaagcgcga cgacctggcc acctggaaca tcggcgccaa ggtcggctat
      841 gcgggcttca tcctggccgg caactactac gacgacgacg gctcgggctt ccgcgagatc
      901 gagcaagccc aggtgaacgg ttggaccgtc gtcggcacct acgagaccgg cccgatcaag
      961 atcggcctca actacatgac catggaagtc gagcacaacg ccctgtgggc ggcccggacc
     1021 gggttcggca ccgccggcac gatcgcggcg ccgattgggg cacgcaacgc catcaacacg
     1081 ttcgcgctcc cgttcacggc gggcgtgacc ggcgaccgga ccgtggtcga ttcgtacaac
     1141 gccgagggct tcggcggcgg cgtcggctac cagttggcgc cgggcctcaa gctctacgcg
     1201 gacatcggct acttcgactt cgacgcgccg attccggagc tcgagaacaa cggcatcgtc
     1261 ggctacggcg gcatctacgt cagcttctga cgcgcctgtc cgttcgacgg atcggaaggg
     1321 gtggcgggcc ttgtccgcca cccctttttc cgtgcccgcg cgggccggcg ccattgcccc
     1381 ttgttgattt gtgcggcggc gggcatagaa tggcggccat gaggcacctg tgtctgtccg
     1441 tggccctgat tggcttggcg atcgcgactg ccggttgcgg cgacaccgac gtgccggtcg
     1501 aggcgaccta cccgtccgac aacccgcgac cgcccggaac gctgctgggc aagaaccagc
     1561 agacctatta cgagggcaac agcctgttcg gcggcgacgg catcgtgctg ttcggcggcc
     1621 ccaactacga gcccgaccgc agcgacggcg ccggcatcgg ggtgaactcc ttcctgtggc
     1681 gcgcctcgct cgataccttg tcgttcatgc cgctggtctc ggccgacccg ttcgggggcg
     1741 tgatcatcac cgactggtac accccgcccc aggcgcccga cgagcggttc aaggtttccg
     1801 cgttcatcct cgaccgctcc ttgcgcgccg acggcgtacg cgtgtcggtg ttccgccaga
     1861 cccagcagtc gccgggcaac tgggtcgatg cgccgctcgc cgagagcatg gccaccgacc
     1921 tcgagaacgc gatcctggcg cgcgcgcgcc agatgcgcat ccgcaccctc gccagccagt
     1981 agcgctccgc cggcgcgccg cgccaagggc aatggcagtt gacgattgtc cgatgccctg
     2041 cgacgtcttt gggcacgctg aggtgcagcc ttcgagacgc cctcttacga gggctcctca
     2101 ggctgaggag cgccgaaggc gcgtctcgaa gcctccgcgc ctccgcgtga accgagtgac
     2161 cggcggtcat caggcaccgc gctgcgcgcg tggcgatgac gttttctcaa taccctctgt
     2221 gacctctgtg cactctgtgg tttatctttg aatcggcatg agcgaagcgc ggcgatgagc
     2281 cgatacgacc cccagtcaac cgaggccaag tggcagcgcg cgtggcacga tgcgggcgcg
     2341 ttccgcaccg gccgccacgg cgaccggccg ccctactacg tgctcgagat gttcccctac
     2401 ccgtcggggc gcatccacat gggccacgtg cgcgtctaca ccctcggcga cctgctcgcc
     2461 cgctacaagc gcgcgcgcgg cttcgacgtg ctgcacccga tgggctggga cgcgttcggc
     2521 atgccggccg agaacgcggc gatggaggag ggcgtccatc ccgccacgtg gacctggtcg
     2581 aacatcgagg tgatgcgcgg ccagctcaag tcgatggggc tgtcctacga ctggacgcgc
     2641 gagcttgcga cctgctcgcc cgactactat cgccacgagc agaggatgtt cctcgacctg
     2701 ctcgccgcgg gcctggccta ccgcaagcag gcgtgggtca actgggaccc ggtggacaag
     2761 accgtgctcg ccaacgagca ggtgatcgag ggccgcggct ggcgctcggg cgcgccggtc
     2821 gagcggcgcc tgttgccgca gtggctgttc cgcatcaccg ccttcgccga cgacctgctg
     2881 gattcgttgg cgcggctcga ccgctggccc gactcggtgc gcaccatgca ggccaactgg
     2941 atcggccgct ccgacggcgc tgtcgtgcgc ttccgcctgg ccgggcgcga gggccggctc
     3001 gaggtgttca ccacccgccc cgacaccata ttcggcgcga gcttcgttgc catcgcgccc
     3061 aaccatccgc tcgccgccga gcttgcccgc tttgatgcgg cgctcgccgg cttcatcgag
     3121 gactgcaacc gcatcggcac ctcggaggcc gcgctcgaga ccgccgagaa gcagggcttt
     3181 cgcaccgcgc tcgaggccct gcacccgttc cgcgccggcc tgcgcctgcc ggtctacgtc
     3241 gccaacttcg tgctcatgga gtatggctcg ggcgccatct tcggggtgcc cgcgcacgac
     3301 cagcgcgacc tcgagttcgc ccgcaagtac gcgctcccgg tgcgcatcgt cgtccgcccg
     3361 cgcgacgcgg gcgaggactt cgccatcgac ggcgaggcct atgccggcga cggcgtggcg
     3421 gtcaactcgg acttcctcga cgggctctcg gtcgccgacg ccaagggcgc cgtgacccgc
     3481 cgtctggccg cggccggcga cggcgaggcg cgcaccagct atcgcctgcg cgactggatc
     3541 gtctcgcgcc agcgctactg gggctgccca atcccggtca tccattgccc ggggtgcggc
     3601 atcgtgccgg tgcccgcggc cgacctgccg gtgaccttgc ccgaggacgt caccttcgat
     3661 gagcccggca acccgctcga ccgccacccg agctggaagc acgtcgcctg cccgacctgc
     3721 ggcagcgcgg ccgtgcgcga taccgacacg ttcgatacct tcgtcgattc gtcgtggtac
     3781 ttcgcccgct tcgcctcgcc gcacgccgag ctggcgctgg agccgcaggc gatgcgccgc
     3841 tggctgccgg tcgaccagta tgtcggcggc atcgagcacg cgatcctgca cctgctgtac
     3901 tcgcgcttct tcatgcgcgc gctcaggcag tgcggctaca tcgacttcga cgagccgttc
     3961 gccggcctgc tcgcccaagg catggtgtgc cacgagacct accgcgaggg gcgcagctgg
     4021 ctctatcccg agcaggtcga gcgcggcgcg gacggcagcg cccgccgccg cgataccggc
     4081 gcggcggtca ccgtcgggcg ctcggagaag atgagcaagt cgcggcgcaa cgtgatcgac
     4141 ccggccgaca tgatcgcccg ctacggtgcc gataccgcgc gcctgttcat gctctccgac
     4201 agcccgcccg agcgcgcgct cgagtggacc gcggccggca tcgacggcgc cttccgctac
     4261 gtgcaacggc tgtggcgcct cgtcgccgag ccgccggcgg cgctggcgcc gcgcgggacc
     4321 gcgatggcga atcggctcac gcccgccggc ctcgagctcc gccgcgccgt gcaccgaacg
     4381 atcgccgccg tgaccgacga catcgaacgg ttccacttca acaaggcggt cgcacgcgtg
     4441 cgcgaactga ccaacgccgt cgcctcgttc gaggtctcgg ccgccgatgc gggcgcggtg
     4501 ttgcgcgagg cgctcgagac gctggtgctg ctgatcgggc cgatgacgcc gcatctcgcc
     4561 gaggagctgt ggcgagcgct cggccatgac cggttcctgt gcgaccagcc ctggcccgcg
     4621 gcggatcccg cgctggtcgc ggtcgagacc gtggtgatcg cggtccaggt caacggcaag
     4681 aagcgcgcca cggtcgaggt cgtcgccggc agcgacgccg gcaccgtcga ggcgcgcgcg
     4741 ctggccgagc ccaacgtcgc gagcttcatc gccggcaagc cgatccgcaa ggtcatcgtc
     4801 gtgcccgacc gcatcgtcaa tgtggtggtc tagac
//
LOCUS       DASWHU010000365         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2110709, whole genome shotgun sequence.
ACCESSION   DASWHU010000365 DASWHU010000000
VERSION     DASWHU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110709"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VGB88_13605"
     CDS             complement(<1..509)
                     /locus_tag="VGB88_13605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9648535.1"
                     /translation="MNILLVTDAWAPLVNGVARTFAQVRAQCEAKGHRVDVVSPDRFR
                     TVPCPSYPQIPLAVFPGRRLRAVLEKVRPDAVHLATEGPLGLAARNLLVRRALPFTTS
                     YATRFPEYLAARLPVPLRWGYAAMRWFHAPSRAVMVATETLRRELAGHGFANLRTWSR
                     GVDAELFRPG"
     gene            complement(506..1282)
                     /locus_tag="VGB88_13610"
     CDS             complement(506..1282)
                     /locus_tag="VGB88_13610"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX9648536.1"
                     /translation="MAHSRLHRTIFLSDIHLGTRGCRAATLLDFLRHNDAETIYLIGD
                     IVDGWQLKRAWYWPQAHNDVVQKLLRKARKGTRVVYVPGNHDALVRDYCGTHFGGVEV
                     RLDDIHETADGRRLWVVHGDAFDSVVMNARWLAVLGDWSYVNLLRINVAYNALRRALG
                     LPYWSLSAYLKFKVKNAVQYIGNFETAVAAEARKRAVDGVVCGHIHHAEIRHIDGLLY
                     VNDGDWVESCTAAVEDASGQIEIVHWAEPAAQPVARAAAA"
     gene            1388..2284
                     /locus_tag="VGB88_13615"
     CDS             1388..2284
                     /locus_tag="VGB88_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9648537.1"
                     /translation="MRLLLIHNPEAGGRCGRLLARVREGLEARGIAVDELRTDGAGHA
                     RDIAARIAPGRYDRLAVAGGDGTVNEVVNGLAAHAAPPPLALVPLGTANVLAAEIGLA
                     LEAGAVVETLVAGRVRRVSLGEVSGRRFLLMAGVGFDAHVVAGVDAALKARLGKAAYA
                     ASFVRRLVDFDFPRYRVVIDGAARDAASVVVAKARMYGGAHVIAPAARLEAPEFQVVR
                     FLKSGRGAALAAAAALFTGRLAALPGLAVDAARTVVLDGPEGEPVQADGDIVAHLPAT
                     VRIVEGALDLVYPDPAFAIPGL"
     gene            complement(2248..2880)
                     /locus_tag="VGB88_13620"
     CDS             complement(2248..2880)
                     /locus_tag="VGB88_13620"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HEX9648538.1"
                     /translation="MSARRAAHAALAGLSLALGGWQVATGGWIEAKAWLAQRLLEHAW
                     AESRAGAGPVKPWPWADTWPVARLTIPAHGVDLIVLAGGSGRTLAFAPGHVDGSPLPG
                     RPGTSLIGGHRDTHFRVLARLAPGTEIVVEDAAGARHRFRVRSAEVVDARTTRIAAAG
                     TSPGLALVTCYPFDALVPGGPLRWLVLADLASPAEASPGHSPGIAKAGSG"
     gene            complement(2877..>3848)
                     /locus_tag="VGB88_13625"
     CDS             complement(2877..>3848)
                     /locus_tag="VGB88_13625"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HEX9648539.1"
                     /translation="DADGGTEMAPALARALTGAPPQGKLRQVVFLTDGAVGNETELFA
                     IIDDDLGASRLFTVGIGSAPNSYFMAKAAEIGRGTFTHIGETSQVAERMEALFAKLEH
                     PVMTDIDTAWPAGLVPHMSRATLPDLYAGEPVVFTARLDALEGAVTVSGVRDGEPWAA
                     RLDLAQAAPAHGIAALWARDRIDAMLHESGVLMDPAEVQGEVVALALAHHLVTKFTSL
                     VAVDVTPTRPEGAAMASRKLPHNLPYGWDYEKLFGAQPQPAAPLQRDAAAQASGATLA
                     SADAAVVVGTGFGLSLPQGATPAQLHWLIGLALLVIGLAALGRLCPA"
BASE COUNT          517 a         1349 c         1461 g          521 t
ORIGIN      
        1 ccggggcgga acagctcggc atcgacgccg cgcgaccacg tgcgcaggtt ggcgaagccg
       61 tgcccggcaa gctcgcggcg cagggtctcg gtcgccacca tcaccgcccg cgacggcgca
      121 tggaaccagc gcatcgcggc atagccccaa cggagcggca ccggcagccg ggcggccagg
      181 tattcgggga agcgggtcgc gtagctggtg gtgaacggga gggcgcggcg caccagcagg
      241 ttgcgcgcgg cgaggccgag cgggccctcg gtggcgaggt ggacggcatc gggccggacc
      301 ttctcgagca ccgcccgcag ccggcgcccg gggaacacgg cgagcgggat ctgcgggtag
      361 ctcgggcagg ggacggtccg gaaccggtcg ggcgagacca cgtcgacccg gtgccccttg
      421 gcctcgcact gcgcgcgcac ctgggcgaag gtgcgggcga cgccgttgac cagcggtgcc
      481 caggcgtcgg tgaccaggag gatgttcatg ccgccgcggc gcgcgccacg ggctgggcgg
      541 cgggctcggc ccagtggacg atctcgatct ggccggaggc gtcctcgacc gcagcggtgc
      601 agctctcgac ccagtcgccg tcgttgacgt agagcagccc gtcgatatgg cgaatctcgg
      661 cgtggtggat gtggccgcag accaccccgt cgacggcgcg cttgcgcgcc tccgccgcga
      721 ccgcggtctc gaagttgccg atgtactgga ccgcgttctt gaccttgaac ttgagatacg
      781 ccgacagcga ccagtacggc aggccgagcg cgcgccggag cgcgttgtag gcgacgttga
      841 tgcgcagcag gttgacgtag gaccagtcgc cgagcaccgc cagccacctg gcgttcatca
      901 ccacgctgtc gaaggcatcg ccgtggacca cccacaggcg gcggccgtcg gcggtctcgt
      961 ggatgtcgtc gagcctgacc tcgacgccgc cgaaatgggt gccgcagtag tcgcggacca
     1021 aggcgtcgtg gttgcccggc acgtagacca cgcgggtgcc cttgcgcgcc ttgcgcagca
     1081 gcttctggac gacgtcgttg tgtgcctgcg gccagtacca cgcccgcttg agctgccagc
     1141 cgtcgacgat gtcgccgatc aggtagatgg tctcggcgtc gttgtggcgg agaaagtcga
     1201 gtaacgtcgc ggcccggcag ccacgcgtgc cgagatggat gtcggacagg aagatcgtgc
     1261 ggtgcagtcg cgagtgcgcc attgccgatg acaggtatat agcaagttta gattacagaa
     1321 taatgacaat gaattatagt gattcgttgc gacccgacat cgacatgatc gctgacccgc
     1381 cggggccgtg cgcctgctgc tgatccacaa cccggaggcg ggcgggcgct gcgggcggct
     1441 gctcgcgcgc gtccgcgagg gcctcgaggc gcggggaatc gcggtcgacg agctgcgcac
     1501 cgacggcgcc ggccacgccc gcgacatcgc ggcacggatc gctcccgggc gctacgaccg
     1561 acttgccgtg gcgggcggcg acggcaccgt caacgaggtg gtcaacggcc tcgccgcgca
     1621 cgccgcgccg ccgccgcttg cgctggtgcc gctcggcacc gccaacgtgc tcgccgccga
     1681 gatcggtctc gccctcgagg cgggcgcggt ggtcgagacc ctggtggcgg ggcgcgtgcg
     1741 ccgggtgtcg ctgggcgagg tgtcggggcg acgcttcctg ctgatggccg gggtcggctt
     1801 cgacgcccat gtcgtcgccg gcgtcgatgc cgcgctcaag gcccgcctcg gcaaggcggc
     1861 ctatgcggcc tcgttcgtcc gccggctggt cgatttcgat tttccgcgct atcgggtggt
     1921 gatcgacggc gcggcccggg acgcggcctc ggtggtggtc gccaaggcgc gcatgtatgg
     1981 cggcgctcac gtcatcgcgc cggcggcgcg gctcgaggcg cccgagttcc aggtggtgcg
     2041 cttcctcaaa tcggggcggg gcgcggcgct cgccgccgcg gcggcgctgt tcaccggccg
     2101 cctggcggcg ctgccggggc tcgccgtgga cgccgcgcgc accgtggtcc tcgacgggcc
     2161 cgagggcgag ccggtgcagg ccgacggcga catcgtcgcg cacctgccgg cgacggtgcg
     2221 catcgtcgag ggcgcgctcg acctcgtcta tccggacccc gccttcgcga tccctgggct
     2281 gtgacccggg gatgcttcgg cgggcgaagc aaggtcggcc agcaccagcc agcgcagcgg
     2341 cccgcccggc accagcgcat cgaacgggta gcaggtgacc agcgcgagcc ccgggctggt
     2401 gcccgcggcg gcgatccgcg tcgtgcgcgc atccactacc tccgcgctcc gtacccggaa
     2461 tcggtggcgg gcgccggccg cgtcctcgac cacgatctcg gtaccgggcg cgagccgggc
     2521 aaggacgcgg aagtgggtat cgcggtggcc gccgatcagg ctggtgcccg gccggccggg
     2581 cagcggcgag ccgtcgacat gcccgggcgc gaaagccagc gtgcgcccgc tgccgccggc
     2641 gagcacgatc aggtcgacgc cgtgggccgg aatcgtgagc cgcgcgaccg gccaggtgtc
     2701 ggcccacggc cacggcttca ccgggccggc gccggcgcgg ctctcggccc aggcgtgctc
     2761 gagcaggcgc tgggcgagcc acgctttggc ctcgatccag ccgccggtcg cgacctgcca
     2821 cccgccaagg gcgagcgaca ggccggcgag ggcggcgtgc gccgcgcggc gcgcgctcac
     2881 gcggggcaca agcggcccag cgccgcgagc ccgatgacca gcagcgcgag cccgatcagc
     2941 cagtggagct gggccggagt cgccccttgc ggcaggctca ggccgaagcc ggtcccgacc
     3001 accaccgcgg cgtccgcaga ggcgagggtg gcgcccgagg cctgtgcggc ggcgtcgcgc
     3061 tggagtggcg cggcgggctg cggctgcgcg ccgaacagct tctcatagtc ccagccgtaa
     3121 ggcaggttgt ggggcagctt gcggctcgcc atcgccgcgc cctcgggccg ggtcggggtc
     3181 acgtcgaccg cgaccaggct ggtgaacttg gtcaccaggt gatgggcgag ggcgagcgcg
     3241 actacctcgc cctgcacctc ggccgggtcc atgagcacgc ccgactcgtg cagcatcgca
     3301 tcgatgcggt cgcgcgccca cagcgcggcg ataccgtgcg cgggcgcggc ctgggcaagg
     3361 tcgagccgcg ccgcccaggg ttcgccgtcg cgcaccccgg acaccgtcac ggcgccttcg
     3421 agcgcatcga ggcgggcggt gaacaccacc ggctcgccgg cgtagaggtc gggcagggtc
     3481 gcccgcgaca tgtgcggcac cagcccggcc ggccatgccg tgtcgatgtc ggtcatcacc
     3541 ggatgctcga gcttggcgaa cagcgcctcc atgcgctcgg cgacttggct ggtctcgccg
     3601 atatgggtga aggtgccgcg cccgatctcg gccgccttgg ccatgaagta gctgttgggc
     3661 gccgagccga tgccgaccgt gaacagccgg ctcgcgccca gatcgtcgtc gatgatggcg
     3721 aacagctcgg tctcgttgcc gaccgcgccg tcggtgagga acaccacctg gcgcagcttg
     3781 ccctgcggtg gcgcgccggt gagcgcgcgc gccagcgccg gcgccatctc ggtgccgccg
     3841 tcggcatc
//
LOCUS       DASWHU010000366         6648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_988507, whole genome shotgun sequence.
ACCESSION   DASWHU010000366 DASWHU010000000
VERSION     DASWHU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988507"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..580)
                     /locus_tag="VGB88_13630"
     CDS             complement(80..580)
                     /locus_tag="VGB88_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX9648540.1"
                     /translation="MKTHDVSRRPQRRDRLLREHIHDPYKTRLKLDDPTVCPQCGAVY
                     RSDRWQWGIKPADAREELCQACHRINDKYPAAEITVHGAFVARHRQEILDLVRNEEKI
                     ENAEHPLHRIMDIVADEDGLRITTTDIHLPRRIGLALAHAFNAPFDFDYDKEGYFLRG
                     SWRRDD"
     gene            791..1060
                     /locus_tag="VGB88_13635"
     CDS             791..1060
                     /locus_tag="VGB88_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648541.1"
                     /translation="MYLWRLSPIDPADPNWEASSYRGVAVVRARDEAGARKAAETAFG
                     VKTRFPPGHGVTAPPWLRPASVKAEIIEDSRYQSDGPTEVVFPPP"
     gene            complement(1070..1513)
                     /locus_tag="VGB88_13640"
     CDS             complement(1070..1513)
                     /locus_tag="VGB88_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648542.1"
                     /translation="MTPYDVDAPVNAVADWIRTDRLLDQPRLLLQASKEYAFEADFDR
                     AAFGLGDEDDVALVSVVGLLEVQTRRGRKDWTLQLRIEESLGLRAAGDEDDYEDQPAM
                     PFEAFDEQFLATGLGEVEMSVLAETPAAKARFDRWLTRHLKRAGP"
     gene            complement(1540..1956)
                     /locus_tag="VGB88_13645"
     CDS             complement(1540..1956)
                     /locus_tag="VGB88_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEX9648543.1"
                     /translation="MAENEPEIDTAKVCYIVVRAREFEAQEDVVEPDYASDAIDDGFR
                     QVLEAYADDPTFEELRTFIDGLNVDDQCRLVALTWVGRGDYAKGEWDEALGLARERHN
                     ERTSLYLLGMPLLPDYLEEGLAAFDLSCEDFEMGHL"
     gene            complement(2062..3252)
                     /locus_tag="VGB88_13650"
     CDS             complement(2062..3252)
                     /locus_tag="VGB88_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9648544.1"
                     /translation="MAEQGLARERVSRTVRGIAMSAIKEMAMRSARTEGAVSLAWGLP
                     SFRTPDYIRRAVESELEADPQIGMYSLPDGVPELRQAVTRVHRAATGIAVDADRNVMI
                     TAGNMQGLNALFHVIIDPGDEVIVTDPGFSSHIQQIRLCGGRPVYWTLDEARGWALDL
                     GALPGLVGERTKAIVLVSPSNPTGKIFTEDELRRVGEIAAARDVLILLDDPYSHFTYE
                     NRDRYFNLSSVAALAERIAYLFTFSKTYAMSGWRLGYMIVPEALKREVLKVHDATIIC
                     APRISQVAALAALTAEPAHLDEFEAVLARRRALICERLARVAHVFSFLEPEGAYYVFP
                     RIVAAHRDSFEFSIRLLEEAKVSVTPGSAFGPSGESHVRMAYCVAEDVIERAFDRIEQ
                     RFPA"
     gene            3383..5107
                     /gene="polX"
                     /locus_tag="VGB88_13655"
     CDS             3383..5107
                     /gene="polX"
                     /locus_tag="VGB88_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX9648545.1"
                     /translation="MPVPNVEIARILGEIADLLEIEAANPFRVRAYRNAARTVEALPR
                     EVSAMLGAGATLTDLPGIGADLAAKIADIARTGTTRMLDELHREVPATVGELLRLPGL
                     GPKRVKALWHELGVQTADQLARAARDGRVRALPGFGAKTEARLIAAIAALGGGERRMK
                     LATAAAFADALVGYLEAVPGVGRVIVAGSFRRGRETVGDLDILVTAAAGSPVMERFVA
                     YDDVARVVAQGPTRATVVLRGGLQVDLRVVPERSYGAALHYFTGAKAHNIAIRALGQR
                     RGLKINEYGVFKGKRRIAGETEASVYAAIGLPWIAPELRESRGEIEAARAGRLPKLVE
                     LSDLAGDLHVHTNATDGHNTLREMALAARERGLRYIAITEHSRRLTVARGLDAKRLLR
                     EVERIDRLNDQGLGVTVLTGIEVDILEDGRLDLPDSVLARLDLVVAAVHSAFGLSRDK
                     QTARILAAMDRPHFTILAHPTGRLIAEREPYDVDMERIIRAARARPCYLELNAHPDRL
                     DLLDTHCRMAKEEGVLVSINSDSHRTDGFDNLRFGIGQARRGWLEAKDVLNTRPLAQL
                     RRLLARTM"
     gene            5199..5873
                     /locus_tag="VGB88_13660"
     CDS             5199..5873
                     /locus_tag="VGB88_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9648546.1"
                     /translation="MIPSSATGGAAGAAVTIPPHGLEGLLGLPRAPGGVILFAHGSGS
                     SRFSPRNTYVARALQDAGFATLLFDLLRADEQDDRANVFDIALLSERLEQAIAWVAAD
                     KRTRGLAVGLFGASTGAAAALVAAARRGDVVRAVVSRGGRPDLAGAALARVRAPTLLI
                     VGGADSQVLDLNRAALAMITAEARLEVVPGATHLFEEPGTLDGVIAAASAWFRCHLRA
                     VAEAPR"
     gene            5870..6574
                     /locus_tag="VGB88_13665"
     CDS             5870..6574
                     /locus_tag="VGB88_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX9648547.1"
                     /translation="MSEPVMFLDRADAGRRLAQKLGMWRGRDAVVIALPRGGVPVAAE
                     IARVLEAPLDLVLVRKIGAPFQPELAIGAVVDGEEPELVLNQDVVAALSVPERYIEAE
                     RKRQVAEIERRRTLYFAGRERVSPAGRPVILVDDGIATGATARVAIHALKRKGPARLI
                     LAVPVAPPDSAAELAREVDELVCLETPEQFFAISQFYADFPQVSDQEVSDLLSRQRPP
                     EGAVAARPQSQPSTRS"
BASE COUNT          933 a         2366 c         2375 g          974 t
ORIGIN      
        1 ggaccccgga taacggcttc gccgtttccg gggtgacgac ttagaaaatc ccagcgattc
       61 gagcaaatat catcccgttc taatcgtcgc ggcgccagct gcccctgagg aagtagcctt
      121 ccttgtcgta gtcgaaatcg aacggcgcat tgaaggcgtg cgcgagcgcc aagccgatgc
      181 ggcgcggcag atggatgtcg gtggtggtga tgcgcaagcc gtcctcgtcg gcgacgatgt
      241 ccatgatgcg gtgcagcggg tgctccgcgt tctcgatctt ctcctcgttg cgtacgagat
      301 cgaggatctc ctgccggtgc cgggcgacga aggcgccgtg tacggtgatc tcggcggccg
      361 gatacttgtc gttgatgcgg tggcaggcct ggcacagctc ttcccgggcg tcggcgggct
      421 tgatgcccca ctgccagcga tcgctgcgat agaccgcgcc gcattgcggg cacaccgtcg
      481 ggtcgtcgag cttcagccgc gtcttgtagg gatcatggat gtgctcgcgc agcaggcgat
      541 cgcggcgctg cggccggcgc gaaacgtcgt gggtcttcat ggcggggcaa cctcgagggt
      601 aggcggggcc aggatgggcg agtcgatgta tatcagactg cggcggttcg tggcccggtt
      661 caatgaccca gatcagtccc gcgcgggtcc tgccgctcgg gttcttgaca tggatttgga
      721 cacggcgggt cggaatcggc taggagtcgc gtgccggggc tcgagtcccg atggtggagg
      781 cgtgacaggc atgtatctct ggcggctgag cccgatcgac cccgccgacc ccaactggga
      841 ggcgagctcc taccgcggcg ttgcggtggt cagggcccgc gacgaggcag gcgcgcgcaa
      901 ggcggcggag acggcgttcg gcgtcaagac tcgcttcccg cccggccacg gcgtgaccgc
      961 cccgccgtgg ctgcggcccg cctcggtcaa ggcggagatc atagaggact cgcgctacca
     1021 gtcggacggg ccgaccgagg tcgtgttccc gccgccatga gggcgcgggc tagggacctg
     1081 cccgcttcag gtgccgcgtc agccagcggt cgaagcgcgc cttggcggcc ggggtctccg
     1141 ccagcaccga catctcgacc tcgcccaggc cggtggcgag gaattgctcg tcgaacgcct
     1201 cgaacggcat cgccggctgg tcctcgtagt cgtcctcgtc gccggccgcg cgcaggccga
     1261 gcgactcctc gatcctgagc tgaagggtcc agtccttgcg cccgcgccgc gtctgcacct
     1321 cgagcaggcc cacgaccgag accagggcga cgtcgtcctc gtcgccgagg ccgaaggcgg
     1381 cgcgatcgaa gtcggcctcg aacgcgtact ccttcgacgc ctgcagcagc agccgcggct
     1441 ggtcaagcag ccggtcggtg cgaatccagt ccgccaccgc gttcaccggc gcatcgacgt
     1501 catagggtgt cattatcggc gttcccgatc gcgcggccct tacaggtgcc ccatctcgaa
     1561 gtcctcgcac gacaggtcga acgcggcgag gccttcctcc aggtagtccg gcagcagcgg
     1621 catgccgagc aggtaaagcg acgtgcgttc gttgtgccgc tcgcgggcca ggccgagcgc
     1681 ctcgtcccac tcgcccttgg catagtcgcc gcgcccgacc caggtgagcg cgaccagccg
     1741 gcactggtcg tcgacattga ggccgtcgat gaaggtgcgc agctcctcga aggtgggatc
     1801 gtcggcatag gcctcgagca cctggcggaa cccgtcgtcg atcgcgtccg aggcgtaatc
     1861 gggctcgacc acgtcctcct gggcctcgaa ctcgcgggcc ctgacgacga tgtagcacac
     1921 cttggcggtg tcaatttccg gctcgttttc agccatcatc cgtcctcatc aacttcgccg
     1981 ccccggtcga atcgggcaca tgctgtggcg tgcgcgcggg gcgctccatg atctggatca
     2041 gctcgccggc cgagtcgcgc ctcatgccgg gaaccgctgc tcgatccggt cgaaggcgcg
     2101 ctcgatcacg tcctcggcga cgcagtatgc catgcggacg tggctttcgc cgctcgggcc
     2161 gaaggcgctg ccgggggtga cgctgacctt cgcctcctcg agcaggcgga tcgagaactc
     2221 gaacgagtcc ctgtgggcgg cgacgatcct cggaaacacg taataggccc cctccggttc
     2281 gaggaagctg aacacgtgcg ccacgcgcgc gagccgctcg cagatcagcg cccgccggcg
     2341 ggcgagtacc gcctcgaact cgtccagatg cgcgggctcc gcggtgagcg cggcaagcgc
     2401 cgcgacctgc gagatgcgcg gcgcacagat gatcgtcgcg tcgtgcacct tgagcacctc
     2461 gcgcttgagc gcctcgggca cgatcatgta gccgagccgc cagccgctca tggcgtaggt
     2521 cttggagaag gtgaacagat aggcgatgcg ctcggcgagc gcggcgaccg aggacaggtt
     2581 gaagtagcga tcccggttct cgtaggtgaa atgcgagtag gggtcgtcga gcaggatcag
     2641 cacatcgcgc gcggcggcga tctcgccgac ccgcctgagc tcgtcctcgg tgaagatctt
     2701 gccggtcggg ttcgacggcg agaccagcac gatcgccttg gtccgctcgc ccaccaggcc
     2761 cggcagcgcg ccgaggtcga gcgcccagcc ccgcgcctcg tcgagcgtcc agtacaccgg
     2821 cctgccgccg cacagccgga tctgctggat gtgcgacgag aaccccgggt cggtgacgat
     2881 gacctcgtcg cccgggtcga tgatgacgtg gaacagcgcg ttcaggccct gcatgttgcc
     2941 ggcggtgatc atcacgttgc ggtcggcatc gacggcgatc ccggtcgcgg cgcggtggac
     3001 gcgggtgacc gcctggcgca gctcgggcac gccgtcgggc agcgagtaca tgccgatctg
     3061 cggatcggcc tcgagctcgc tctcgaccgc gcgccggatg tagtcgggag ttcggaacga
     3121 cggcagcccc caggcgagcg agaccgcacc ctcggtgcgc gcgctccgca tcgccatctc
     3181 cttgatcgcg gacatggcga tgccgcgcac ggtgcgcgac acccgctcgc gggccaatcc
     3241 ctgctccgcc atctgccgtc cccccgttgc ccgagctcag ccgacagtag cgcgttcgcg
     3301 gcggcgctcg gaagcgtgcg ccggctccgc ggtcggcgca aggtctggcg cgccgcgcgc
     3361 ctgcctgcga tgctgcactg cgatgccggt gcccaacgtc gagatcgcgc gcatcctcgg
     3421 cgagatcgcc gatctcttgg agatcgaggc cgccaacccg ttccgcgtgc gcgcctaccg
     3481 caatgcggcg cgcaccgtcg aggccctgcc gcgcgaggtc tccgcgatgc tgggggccgg
     3541 cgcgacgctc accgaccttc ccggcatcgg cgccgatctc gcggccaaga tcgccgacat
     3601 cgcgcgcacc ggcaccacgc gcatgctcga cgagctgcac cgcgaggtgc cggccacggt
     3661 cggcgagctg ctcaggctgc ccggcctcgg cccgaagcgg gtcaaggcgt tgtggcacga
     3721 gctcggggtc cagaccgcgg accagctggc gcgcgccgca cgcgacgggc gcgtccgcgc
     3781 gctgccgggc ttcggggcca agaccgaggc gcgcctgatc gcggcgatcg cggcgctggg
     3841 cggcggcgag cggcggatga agctcgccac cgccgccgcc ttcgccgatg cgctggtcgg
     3901 ctatctcgaa gcggtgccgg gcgtcggccg ggtgatcgtg gccggcagct tccggcgcgg
     3961 gcgcgagacc gtgggcgacc tcgacatcct ggtcacggcg gccgccggca gcccggtgat
     4021 ggagcgcttc gtcgcctatg acgacgtcgc ccgggtggtc gcgcagggcc cgacgcgcgc
     4081 gaccgtggtg ctgcgcggcg gcctgcaggt ggacctgcgc gtggtgcccg agcggagcta
     4141 cggcgccgcg ctccactact tcaccggcgc caaggcgcac aacattgcca tccgcgcgct
     4201 cggccagcgg cgcgggctca agatcaacga gtacggcgtg ttcaagggca agcggcgaat
     4261 tgccggcgag accgaggcca gcgtgtacgc cgccatcggc ctgccgtgga tcgcgcccga
     4321 gctgcgcgag agtcgcggcg agatcgaggc cgcgcgcgcc ggccggctgc cgaagctggt
     4381 cgagctctcc gacctcgccg gcgacctgca cgtccacacc aatgccaccg acggccacaa
     4441 caccttgcgc gagatggcgc tcgccgcgcg cgagcgcggg ctccgctaca tcgccatcac
     4501 cgagcactcg cggcggctca ccgtcgcccg cggcctcgac gccaagcggc tgctgcgcga
     4561 ggtcgagcgg atcgaccggc tcaacgacca ggggctcggc gtcaccgtgc tcacgggcat
     4621 cgaggtcgac atcctcgagg acggccgcct cgacctgccc gattccgtgc tcgcgcggct
     4681 cgacctggtg gtcgcggccg tgcacagtgc cttcgggctg tcgcgggaca agcagaccgc
     4741 gcgcatcctc gccgccatgg accggccgca tttcaccatc ctcgcccatc cgacgggacg
     4801 gctgatcgcc gagcgggagc cgtacgacgt cgacatggag cgcatcatcc gcgccgcgcg
     4861 cgcccgcccg tgctaccttg agctcaacgc ccaccccgac cgcctcgatt tgctcgacac
     4921 ccattgccgg atggcgaagg aggaaggggt gctggtcagc atcaactcgg actcccatcg
     4981 taccgacggc ttcgacaacc tccgcttcgg catcggccag gcgcggcgcg gctggctcga
     5041 ggcgaaagac gtgctcaaca cccgcccgct ggcgcaattg cgccgcctgc tcgcgcgcac
     5101 catgtgacgc gccggcgggg cgcggactga cctgcatcaa ggcgcccgcg cgccgtggcg
     5161 ccgagcatcg cgcgagtgga ctcgacaggg aggcgaccat gatcccgtca agcgcgaccg
     5221 gcggcgcggc cggcgcggcg gtgaccatcc cgccgcacgg gctggaaggg ctgctcggcc
     5281 tgccgcgcgc ccccggcggg gtgatcctgt tcgcccacgg cagcggcagc agccgcttca
     5341 gcccgcgcaa cacctatgtc gcgcgcgcgt tgcaggacgc gggcttcgcc accctgctgt
     5401 tcgacctgtt gcgcgcggac gagcaggacg accgcgccaa cgtgttcgac atcgcgctgc
     5461 tgtccgagcg cctcgagcag gcgatcgcct gggtcgcggc ggacaagcgc acgcggggtc
     5521 tcgcggtcgg gctgttcggc gccagcacgg gcgcggcggc ggcgctggtc gcggcggcgc
     5581 gccggggcga cgtggtgcgc gcggtggtct cgcgcggcgg ccggcccgat ctcgccggcg
     5641 cggcgctggc gcgcgtgcgc gcgccgacct tgctgatcgt cggcggcgcc gatagccagg
     5701 tgctcgacct caaccgcgcg gcgctggcga tgatcacggc tgaggccagg ctcgaggtcg
     5761 tgcccggcgc cacccacctg ttcgaggagc cgggcacgct cgatggcgtg attgccgcgg
     5821 ccagcgcgtg gttccggtgc cacctgcgcg cggtcgcgga ggcgccgcgg tgagcgagcc
     5881 cgtgatgttc ctcgaccggg ccgacgccgg ccgccggctg gcccagaaac tcggcatgtg
     5941 gcgcgggcgc gacgccgtgg tcatcgcctt gccgcgcggc ggcgtgccgg tcgcggcgga
     6001 gatcgcgcgc gtcctcgagg cgccgctcga cctggtgctg gtgcgcaaga tcggcgcccc
     6061 gttccagccc gagctcgcga tcggcgcggt ggtcgacggc gaggagccgg agctggtgct
     6121 gaaccaggac gtggtcgccg ccttgagcgt gcccgagcgc tatatcgagg ccgagcgcaa
     6181 gcgccaggtg gctgagatcg agcgccggcg cacgctctac ttcgccgggc gcgagcgggt
     6241 gagccccgcc gggcggccgg tgatcctggt cgacgacggc atcgccaccg gcgccaccgc
     6301 gcgggtcgcg atccatgcgc tcaagcgcaa gggcccggcg cgcctgatcc tcgccgtccc
     6361 ggtcgccccc ccggacagcg ccgccgagct tgcgcgcgag gtcgacgagc tggtctgcct
     6421 ggagacgccg gaacagttct tcgccatcag ccagttctac gccgacttcc cgcaagtctc
     6481 cgaccaggag gtgagcgacc tgctgtcgcg ccagcgcccg cccgagggcg cggtcgcggc
     6541 gcggcctcag tcccagccga gcaccaggtc gtagccgtcg cgcgcggcga tgcgggtgcc
     6601 gaaccgccgg cactcgcgct cgatcacctc caagagccag gcgatgtc
//
LOCUS       DASWHU010000367         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3218249, whole genome shotgun sequence.
ACCESSION   DASWHU010000367 DASWHU010000000
VERSION     DASWHU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218249"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VGB88_13670"
     CDS             <1..1097
                     /locus_tag="VGB88_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9648548.1"
                     /translation="GSHGPSDELKGELMRRARTAGLLSPSVGREWGGLGLDMRGMAVV
                     FEAAGYSLLGPQALNCAAPDEGNMHLLEQVASADQKAQWLRPLAAGEMRSCFSMTEPA
                     PGAGSDPNMLATTARPDGNHWLIRGHKWFITGFDGAKLNIVMARTAERIDKAKGATMF
                     LVDNDDPNIERVRVQDSMDASFAGGHCEIRFNDVRVRADQVLGEIGEGYRYAQVRLAP
                     ARLTHCMRWLGAARRAHDIACAYAGVRESFGKVLAEHQGVGFMLADNEMDLYASRLAI
                     WHTAWLLDRGDEARQESSMSKVLCAEALFRVADRSMQILGGMGITSDTLVERLWREIR
                     PFRIYDGPSEVHRHAIASRLLRRARKARAG"
     gene            1191..>1605
                     /locus_tag="VGB88_13675"
     CDS             1191..>1605
                     /locus_tag="VGB88_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9648549.1"
                     /translation="MGIGSDGLKALTAAIFSNTGSGAAEAEAIARHLVEANLVGHDSH
                     GVIRVPAYVQWVGEGKLVPNQSISVTFENDSVAIVDGNSGFGQVIGEQAVDLGIDKCR
                     TGGLAMVGLRNAGHIGRIGDWAIRAADAGLVSIHFV"
BASE COUNT          252 a          573 c          568 g          212 t
ORIGIN      
        1 agggcagcca cggtccctcg gacgagctca agggcgagct gatgcggcgc gcccgcaccg
       61 ccggcctgct gagcccgagc gtcgggcgcg aatggggcgg gctcggcctc gacatgcgcg
      121 gcatggcggt ggtgttcgag gccgccggct actcgctgct cgggccgcag gcgctcaact
      181 gcgccgcccc cgacgagggc aacatgcacc tcttggagca ggtcgcgagc gccgaccaga
      241 aggcgcagtg gctgcgcccg ctcgccgccg gcgagatgcg ctcgtgcttc tcgatgaccg
      301 agcccgcgcc aggcgccggc tccgacccca acatgctcgc caccaccgcg cggcccgacg
      361 gcaaccactg gctgatccgg gggcacaagt ggttcatcac cggcttcgac ggcgccaagc
      421 tcaacatcgt catggcgcgc accgccgagc gaatcgacaa ggccaagggt gcgaccatgt
      481 tcctggtcga caacgacgat cccaacatcg agcgcgtccg cgtgcaggac tcgatggacg
      541 cgagcttcgc cggcggccac tgcgagatcc ggttcaacga cgtgcgcgtg cgcgccgacc
      601 aggtgctggg cgagatcggc gaaggctacc gctacgccca ggtccggctc gcgccggcgc
      661 ggctgaccca ctgcatgcgc tggctgggcg ccgcccggcg cgcccacgac atcgcctgcg
      721 cctacgccgg cgtgcgcgag tccttcggca aggtgctggc cgagcaccag ggcgtcggct
      781 tcatgctcgc cgacaacgag atggacctct atgccagccg gctcgccatc tggcacaccg
      841 cctggctgct cgaccggggg gatgaggcgc gccaggaatc gtcgatgtcg aaggtgctgt
      901 gcgcggaggc gctgttccgc gtcgccgacc gcagcatgca gatcctgggc ggcatgggca
      961 tcacctccga caccctggtc gagcggctgt ggcgcgagat ccgcccattc cgaatctacg
     1021 acggcccgtc cgaggtccac cgccacgcca tcgccagccg gctgctgcgc cgcgcgcgca
     1081 aggcccgggc cgggtgatcc cggcattgcc ggcttgcccg tgccgggtgg gacggctaag
     1141 agagcgcgcg caacgaccaa ccaacaacca agcacacggg gtcgacaggc atgggcatcg
     1201 ggagcgacgg gctgaaggcc ctgacggcgg cgatcttctc caacaccggc agcggcgccg
     1261 ccgaggccga ggccatcgcc aggcacctgg tcgaggccaa cctggtcggc cacgacagcc
     1321 acggcgtgat ccgggtcccg gcctacgtcc agtgggtcgg cgagggcaag ctggtgccca
     1381 accagtcgat ctcggtgacg ttcgagaacg actcggtggc gatcgtcgac ggcaactcgg
     1441 gcttcggcca ggtgatcggc gagcaggcgg tcgacctcgg catcgacaag tgccggaccg
     1501 gcgggctggc gatggtcggc ctgcgcaatg ccgggcatat cgggcgcatc ggcgactggg
     1561 cgatccgcgc cgccgatgcc ggcctggtgt cgatccactt cgtca
//
LOCUS       DASWHU010000368         6168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2114071, whole genome shotgun sequence.
ACCESSION   DASWHU010000368 DASWHU010000000
VERSION     DASWHU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114071"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1259)
                     /locus_tag="VGB88_13680"
     CDS             complement(276..1259)
                     /locus_tag="VGB88_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX9648550.1"
                     /translation="MTAPITYLEAARAALAEEMRRDPTVWALGEDLGRGGIFGQYRGL
                     AEEFGPARIVDAPISEAAIMGAAVGAALAGSRPVVEMRFTDFALCAQDELVNQAAKAR
                     YMFGGQARVPMVVRQPMGLWRSSAAQHSQSLESWWVHVPGLVVVAPATPGDTYGLLKS
                     AIRCDDPVVYLEHKDLWRIEGEVAAGSEPVPIGRARIARQGGDATIVTWSRMVHVAEA
                     AADALAADGVAAEVIDLRSLWPWDRDAVFASVRRTGRLLVAHEAVRAGGFGAEVAATV
                     AEELGRDLRAPVRRLGAPRVPIPYARPLEDACRVSADDIAGAVRAMIAAPG"
     gene            complement(1256..2239)
                     /locus_tag="VGB88_13685"
     CDS             complement(1256..2239)
                     /locus_tag="VGB88_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9648551.1"
                     /translation="MTLSRDNMDRTRILGHLRMMARIRAFEVEAEAAQKAGHVTGGIH
                     QSIGQEAVAAGVCANLRRDDLLTSNHRGHGHALAKGADAVAMMCELFGKAAGTCQGKG
                     GSMHIADFAAGMLGANGVVGAGITIAVGAAHALRLRGARSIVACLFGDGAVNRGPFLE
                     GLNWAAVHALPVLFVCEDNGFAALTRTSTLTAGPGVGARAESLGVPARAVDGNDVVAV
                     DDLAGELAAEVRAGAGPRLLHARTYRLKGHTTADAGLYRSADEVAAHARDEPIGRCRA
                     LLSDLGAADAALDRIIAEAAAEMAAAAAAAAAAPWPAPALAFTDVQDIGAP"
     gene            2326..3138
                     /locus_tag="VGB88_13690"
     CDS             2326..3138
                     /locus_tag="VGB88_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648552.1"
                     /translation="MPDVLAADSVTHLDSRHRDQVVLGGSHGGVYAGYLAAKAGVRAV
                     ILSDAGVGKDQAGIGALAYLDALGMAAATVGHMSARIGDGADFLARGVISHVNRTAAG
                     VGCAPGQRCRDCAEALRAAPAWHGAPPDYEEGRVLLRERAGEPEVWGLDSASLLAAGD
                     EDRVLITGSHGALPGGQAELALGVRARAAVFNDAGVGIDRAGIARLAALDAIGVPAAT
                     VAAMSARIGDARSTWDDGRVSYVNDLAAALGARPGMRTPALADLVAAGSRRR"
     gene            complement(3141..4094)
                     /locus_tag="VGB88_13695"
     CDS             complement(3141..4094)
                     /locus_tag="VGB88_13695"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX9648553.1"
                     /translation="MNDAIPDSTVAHAARSGLERLLAAGRFVVTAETTPPLSASPAPL
                     LAQCAPLKGVADAVNVTDGAGAKAHMSNVAAAALLVECGIEPILQVTTRDRNLIALES
                     SLLGAAALGVRNVLCLRGDDVATGDQPEASQVHDLDSRGLLELARVMRDDGRLPSGRR
                     IVDPPRLFLGAADAPAEPGPDWSAGAVEAKIAAGAQFFQTQFCFDLAMLRRYLARLGD
                     AGVLERTRYLIGIGPLRSAGSARWMNHHLFGVNVPEQTIARLAGARDAAAEGIDICVE
                     LIEGLCAMPGVAGAHLMGPGCEQAAAEAIRRSGVLDARARG"
     gene            4216..5544
                     /locus_tag="VGB88_13700"
     CDS             4216..5544
                     /locus_tag="VGB88_13700"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX9648554.1"
                     /translation="MGLRAGCAGAIVALVLAAWTAAAAESADPAALSGGPDATVFRAG
                     RDAFSAPMPVLDGERLRSFDAGRRLFRTVWRPAAVSADSSAVGLGPLFNRNACSGCHV
                     RDGRGRPPEHDGAPARGLVARLAPAGADYGVQLQDQAVVGVAYEGRLALRYDEVEGRF
                     ADGGTFSLRAPRAGIDRPAYGPVDGLAVSLRVPPAVAGVGLLEAVDEAALRALADPDD
                     ADGDGISGRLAEITRAGRREIGRFGWKATRARLADQVAAAAADDIGLASAARPDGGCA
                     PAQVAGLAACRQEVEIDDAQIGALVAYLRGLAVPARRDLDAPAVVGGEALFAAAGCPA
                     CHVPVLPLASGAAIRPYTDLLLHDLGEGLADEAGEGAATGAEWRTAPLWGIGLVEVVN
                     GHSFFLHDGRARSLIEAVLWHGGEAAAARDAVLALSADDRAALVAFLGSL"
     gene            5637..6089
                     /locus_tag="VGB88_13705"
     CDS             5637..6089
                     /locus_tag="VGB88_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012164468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX9648555.1"
                     /translation="MLSRIAGALAALLLVVLAATPALAGGPVNKTMLGTAIDGYDAVA
                     YHTDGRPVEGSSEFAYQWLGATWRFASAENRDLFAADPETYAPAYGGYCAYGVAKGAK
                     PGIDPEAWAIVDDRLYLNLSKKVQGLWQQDIPGYIAKADQLWPTIKDE"
BASE COUNT          749 a         2323 c         2338 g          758 t
ORIGIN      
        1 ggcgtgccct ggccgaacgg ctgccgggag ccgtcgggca gggtgacctc gaacggcacc
       61 gggcagctgg cgaaccggct cgcgaacttg ccgagcagcg agcccgcccg cgtcttggcg
      121 ggcgtggatg acggtgcgtg cccgcgccgt atggtggtct gctccgtcat ggctcctcct
      181 gccggtgcgt cctcgcgccg gccggcacgg ggaacgcata ccacacgaca atacccaaac
      241 gcgcgcgccg aggccacgat cgcccgcggg cgccgctatc ccggtgctgc gatcatggcg
      301 cggacggcgc cggcgatgtc gtcggccgag acccggcagg cgtcctcgag cgggcgcgcg
      361 taagggatcg gcactcgcgg cgcgcccagc cggcgcaccg gcgcgcgcaa gtcgcggcca
      421 agctcctcgg cgaccgtcgc cgcaacctcg gcgccgaagc cgcccgcgcg caccgcctcg
      481 tgggcgacca gcaggcggcc ggttcggcgc accgaggcga acaccgcgtc gcggtcccac
      541 ggccacaggc tcctgaggtc gatcacctcg gcggcgacgc cgtcggcggc gagcgcgtcc
      601 gccgcggcct cggcgacgtg gaccatgcgc gaccaggtga cgatggtggc gtcgccgcct
      661 tggcgggcga tgcgcgcgcg cccgatcggc accggctcgg acccggcggc gacctcgccc
      721 tcgatgcgcc acaaatcctt gtgctcgaga taaaccaccg ggtcgtcgca gcggatcgcc
      781 gatttcagca ggccgtaggt gtcccccggc gtcgccggcg ccaccaccac cagccccggc
      841 acgtgcaccc accagctttc caaggactgc gagtgctgcg cggccgacga ccgccacagg
      901 cccatcggct ggcgcaccac catcggcacg cgcgcctggc cgccgaacat gtagcgcgcc
      961 ttggccgcct ggttgaccag ctcgtcctgg gcgcacagcg cgaagtcggt gaagcgcatc
     1021 tcgaccaccg gccgcgagcc ggcgagcgcg gcgccgaccg cggcgcccat gatggcggcc
     1081 tcggagatcg gcgcatcgac gatgcgcgcc ggcccgaact cctccgccag gccccggtac
     1141 tggccgaaga tgccgccgcg gccgaggtcc tcgccgagcg cccacacggt cggatcgcgg
     1201 cgcatctcct cggccagcgc cgcgcgcgcg gcctccagat acgtgatcgg cgcggtcacg
     1261 gcgcgccgat gtcctggacg tcggtgaagg cgagcgcggg cgcgggccac ggcgcagcgg
     1321 cggccgccgc ggccgccgcc gccatctcgg ccgcggcctc ggcgatgatc cggtcgagcg
     1381 cggcgtcggc tgcgccgagg tcgctgagca gcgcgcggca gcgcccgatc ggctcgtcgc
     1441 gggcatgcgc cgcgacctcg tcggcgctgc ggtaaaggcc ggcgtcggcg gtggtatggc
     1501 ccttcaggcg ataggtgcgc gcgtgcagca ggcgcgggcc ggcgccggcg cgcacctcgg
     1561 cggcgagctc cccggccagg tcgtcgaccg caaccacgtc gttgccgtcg accgcgcgcg
     1621 ccggcacgcc gaggctttcg gcgcgcgcgc cgacgccggg ccccgcggtc agcgtgctgg
     1681 tgcgggtgag cgcggcgaag ccgttgtcct cgcacacgaa caggaccggc agcgcgtgca
     1741 ccgccgccca gttgaggccc tccaggaacg ggccgcggtt gaccgcgccg tcgccgaaca
     1801 agcaggcgac gatgcttcgt gcgccgcgca atctgagcgc gtgcgcggcg ccgaccgcga
     1861 tggtgatgcc ggcgccgacc acgccgttgg cgccgagcat gccggcggcg aagtcggcga
     1921 tgtgcatcga gccgcccttg ccctggcagg tgccggcggc cttgccgaac agctcacaca
     1981 tcatcgccac cgcgtccgcg cccttggcca gcgcgtggcc gtggccgcgg tggttgctgg
     2041 tcaagaggtc gtcgcggcgg aggttcgcgc acacgcccgc ggcgaccgcc tcctggccga
     2101 tcgactggtg gatgccgccg gtgacgtggc ccgccttctg tgccgcctcg gcctctacct
     2161 cgaaggcgcg gatgcgcgcc atcatgcgca ggtggccgag gatccgggtc cggtccatgt
     2221 tgtcgcggga gagagtcatc ggcgcggtcc cgaaagacgg tccaatcgtc gccggctacg
     2281 gttatcatcg ttcaccgtcc ccggccacgc cgaagccgca ccgccatgcc cgacgtcctc
     2341 gccgccgaca gcgtcaccca cctggactcg cgccaccgcg accaggtggt gctcggcggc
     2401 tcgcatggcg gcgtctatgc cggctatctc gccgccaagg cgggggtgcg cgccgtgatc
     2461 ctgagcgacg ccggggtggg caaggaccag gccggcatcg gcgcgctcgc ctatctcgac
     2521 gcgctcggca tggcggcggc gacggtcggg cacatgagcg cgcgcatcgg cgacggcgcc
     2581 gacttcctcg cccgcggcgt gatcagccac gtcaaccgca ccgccgcagg cgtcggctgc
     2641 gcgcccggcc agcgctgccg cgactgcgcc gaggcgttgc gcgccgcgcc ggcctggcac
     2701 ggcgccccgc cggactacga ggaaggccgg gtgctgctgc gcgagcgcgc cggcgagccc
     2761 gaggtgtggg ggctcgactc ggcgtcgctg ctggcggcgg gcgacgagga ccgggtcctg
     2821 attaccggct cgcacggggc gctgcccggc gggcaggcgg agctcgcgct cggcgtgcgg
     2881 gcgcgggcgg cggtgttcaa cgacgccggc gtcggcatcg accgcgccgg catcgcccgc
     2941 ctcgccgcgc tcgacgcgat cggcgtgccc gcggcgacgg tggcggcgat gagcgcacgc
     3001 atcggcgatg cccgctcgac ctgggacgac ggccgcgtct cgtacgtcaa cgacctcgcc
     3061 gccgcgctcg gcgcgcgccc cgggatgcgc acgcccgctc tcgccgacct ggtcgccgca
     3121 ggctcgcgcc gccgttagcc tcagccgcgc gcgcgcgcgt ccagcacgcc cgagcggcgg
     3181 atcgcctcgg cggcggcctg ctcgcagccc gggcccatca ggtgcgcccc ggcgacaccc
     3241 ggcatggcgc agaggccctc gatcagctcg acgcagatgt cgattccttc cgcggccgca
     3301 tcgcgcgcgc ccgcgagccg ggcgatggtc tgctcgggga cattgacccc gaacagatgg
     3361 tggttcatcc agcgcgcgct gccggccgag cgcagcgggc cgatgccgat caggtagcgg
     3421 gtgcgctcga gcaccccggc gtcgccgagg cgcgcgagat agcggcggag catggcgagg
     3481 tcgaagcaga actgggtctg gaagaactgg gcgccggcgg cgatcttggc ctcgaccgcg
     3541 cccgcgctcc agtcggggcc cggctcggcg ggcgcgtcgg cggcgccgag gaacagccgg
     3601 ggcggatcga cgatccgccg ccccgacggc aaccggccgt cgtcgcgcat gacgcgcgcg
     3661 agctccagca ggccgcggct gtcgaggtcg tgcacctggc tcgcctccgg ctggtcgccg
     3721 gtggcgacgt cgtcgccgcg caggcacagc acgttgcgca ccccgagcgc ggcggcgccg
     3781 agcagcgagg actcgagcgc gatcaggttg cggtcgcggg tggtgacctg gaggatcggc
     3841 tcgatcccgc actccaccag cagcgccgcg gcggcgacgt tggacatgtg cgccttggcg
     3901 ccggcgccgt cggtgacgtt gaccgcatcc gcgacgccct tgagcggcgc gcactgggcg
     3961 agcaggggcg cgggggatgc cgacaagggc ggcgtggtct cggcggtgac cacgaagcgg
     4021 ccggcggcga gcagccgctc gagcccgctg cgggcggcgt gcgcgaccgt gctgtcgggg
     4081 atggcgtcgt tcatgggcgc atgccgggtc cggcgctgga agcaaggcgc gacaccgtag
     4141 gcgagggccg ggcgagggtc aaaccgatcc tcgccgcggc atccggtcgc gcccgctgcc
     4201 tgctaggatg ggcgcatggg tctgcgtgcc gggtgcgcgg gcgcgatagt cgcgctggtg
     4261 ttggccgcct ggacggcggc ggcggcggag agcgccgatc cggcggcgct gtcgggcggg
     4321 cccgacgcga cggtgttccg tgccggccgc gacgccttct cggcgccgat gccggtgctc
     4381 gacggcgagc gcctccgctc cttcgatgcc ggccggcggc tgttccgcac cgtctggcgg
     4441 ccggcggcgg tgtcggcgga ttcgagcgcg gtcgggctcg ggccgctgtt caaccgcaac
     4501 gcctgctcgg gctgccacgt gcgcgacggc cgcggccgcc cgcccgagca cgacggcgcc
     4561 ccggcgcgcg gcctggtcgc gcggctggcc ccggccggcg ccgactatgg cgtccagctc
     4621 caggaccagg ccgtggtcgg cgtcgcctat gagggccggc tcgcgctccg ctacgatgag
     4681 gtcgagggcc ggttcgccga cggcggcacc ttcagcctgc gcgcgccccg cgccggcatc
     4741 gatcggcccg cctacggtcc ggtcgacggc ctcgccgtgt cgctcagggt gccgccggcg
     4801 gtggccgggg tcggcctgct cgaggcggtc gacgaggcgg cgctgcgcgc gctcgccgac
     4861 cccgacgatg ccgacggcga cggcatctcg gggcggctcg ccgagatcac gcgcgccggg
     4921 cgcagggaga tcggcaggtt cggctggaag gcgacgcgcg cgcgcctcgc cgaccaggtc
     4981 gccgccgccg ccgccgacga catcggcctc gcctcggccg cgcgccccga cggcggctgc
     5041 gcgccggcgc aggtcgcggg cctcgccgcc tgccgccagg aggtcgagat cgacgatgcg
     5101 cagatcggcg ctctggtcgc atatctgcgt ggcctcgcgg tgccggcgcg gcgcgatctc
     5161 gatgcgcccg cggtcgtggg cggcgaggcg ctgttcgccg ccgccggctg tccggcttgc
     5221 catgtgcccg tcctcccact cgcctcgggc gcggccatcc ggccttacac cgacctcctg
     5281 ctccacgacc tgggcgaagg gctggccgac gaagcgggcg agggcgccgc aacgggcgcc
     5341 gagtggcgca ccgcgccgct gtggggaatc gggctcgtgg aggtggtcaa cggccactcg
     5401 ttcttcctcc acgacgggcg cgcgcgctcg ctcatcgagg cggtgctgtg gcatggcggc
     5461 gaggcggcgg cggcgcgcga cgcggtgctc gcgctttcgg cggacgaccg cgccgcgctg
     5521 gtcgcgttcc tgggctcgct gtagcgcgcg catcgacaca atcgcgtgag ggccgcttgt
     5581 gcgccggcgc ccgcgcccgc tagcctcggc gcagccaaac gcaaggagac gcgaccatgc
     5641 tttcgcgcat cgcaggcgcg ctcgccgcgc tgctgctggt ggtgctcgcg gccaccccgg
     5701 cgctcgccgg cggcccggtc aacaagacca tgctcggcac cgcgatcgac ggctacgacg
     5761 cggtcgccta ccacaccgac ggccgcccgg tcgaaggctc gagcgagttc gcgtaccagt
     5821 ggctgggcgc gacctggagg ttcgccagcg ccgagaaccg cgacctgttc gccgccgacc
     5881 cggagaccta cgctcccgcc tacggcggct attgcgccta cggcgtcgcc aagggcgcca
     5941 agcccggcat cgaccccgag gcgtgggcga tcgtcgacga ccgcctgtac ctcaacctga
     6001 gcaagaaggt gcaggggctg tggcagcagg acatcccggg ctacatcgcc aaggccgacc
     6061 agctctggcc gaccatcaag gacgagtagc gcgtccgccc gccgggtcgc gaccgaacac
     6121 cgctgagggc ggggcgaaaa ccccgccctc ttgcgcacag accgacga
//
LOCUS       DASWHU010000369         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_991671, whole genome shotgun sequence.
ACCESSION   DASWHU010000369 DASWHU010000000
VERSION     DASWHU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991671"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGB88_13710"
     CDS             <1..379
                     /locus_tag="VGB88_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017011026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9648556.1"
                     /translation="LVIGAGTDGYVSYTQRGSFAIIEDTLRAIVELFPIFSRVRLMRH
                     WGGIVDIAPDASAIISRTPVDGLYVNCGWGTGGFKATPGSGHLFAHTIARDEPHPLAA
                     PFSLDRFARGALVAEHGAAGVAH"
     gene            397..765
                     /locus_tag="VGB88_13715"
     CDS             397..765
                     /locus_tag="VGB88_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX9648557.1"
                     /translation="MLLISCPWCGARERTEFRCGGEAHIVRPKDPAALTDEQWADYLF
                     AKTNPKGLHYERWHHAHGCRRWFNVARNTADDRILAVYRMGEPPPAIGGQAPATPCGE
                     PDLGSGNRALSPPGPGADTR"
     gene            762..>3428
                     /locus_tag="VGB88_13720"
     CDS             762..>3428
                     /locus_tag="VGB88_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEX9648558.1"
                     /translation="MTGQRFRCAEGGRIDRARPLAFTFDGRAYQGFAGDTLASALVAN
                     GVRLVGRSFKYHRPRGIFAAGADEPNALVRLGEGGRTEPNIRATEVELYDGLIACSQN
                     RWPSLAHDLGAVIGLAAPLVPSGFYYKTFMWPPALWRRLYEPLIRRAAGMGRAPEAPD
                     PDSYDHMHGHCDVLVAGGGAAGLAAALAAGRAGARVVLAEREPEFGGLLLSEPPGEAR
                     IDGMPAIDWVAQAVAELSSLPEVRLLPRTTAFGYHLDNYLCLLERLTDHFPPNQAPAG
                     VPRQRLWTVRARQVVLAAGALERPLVFAGNDRPGVMLAGAARSYVNRFAASPGRRVVV
                     LANHDGGYLCARDLARAGIKVRALIDVRERASDTLAAPLRAAGTEVLAGHAIVGTAGR
                     HRLRAVQVAPLAADGARREIGCDLLCVSGGWNPAVHLFSHSRGTLRFRADDGVFVPDR
                     SHQAERSAGACNGTFALSGCLAEGLAAGQDAARRAGFESAAIAPPAVEAAGEARATPT
                     WLLPADSKAFVDVQNDVTAADLGLAVREGYRSIEHVKRYTTTGMGTDQGKTGNVNALA
                     IVAAARGEDIPAVGVTTFRPPYAPVSFGAVAGREVGQFYQPLRTTPMHAWHQAHGAAF
                     EPVGQWHRPWYYARPGETMQRAVMREAKAVRAGVGVLDATTLGKIDVQGRGAVEFLNR
                     IYINAWTKLGIGRCRYGVMLDDQGMVMDDGVTTRLGETHFHLTTTTGGAAPVLAWLEE
                     WLQTEWPEIEVYLTSVTEQWAVIALAGPDSRKLLARLAEGIDLSGEAFPHMAMREGVV
                     AGVAARVFRISFSGELGYEVNVPASYGLAVWQAVIAAGAEFALTPYGTETMHLLRAEK
                     GYIMVGQESDGTVTPIDLGLERMIGRTKADFIG"
BASE COUNT          437 a         1286 c         1241 g          464 t
ORIGIN      
        1 gctggtgatc ggcgccggca ccgacggcta cgtctcctac acccagcgcg gctcgttcgc
       61 gatcatcgag gacaccctgc gcgccatcgt cgagctgttc ccgatcttca gccgggtgcg
      121 gctgatgcgc cattggggcg gcatcgtcga catcgccccg gacgccagcg cgatcatctc
      181 gcgcacgccg gtggacggcc tgtacgtcaa ctgcggctgg ggcaccggcg gcttcaaggc
      241 gacgccgggc tcggggcatc tgttcgcgca caccatcgcc cgcgacgagc cgcacccgct
      301 cgccgcgccg ttctccctcg accggttcgc gcgcggcgcg ctggtcgccg agcacggcgc
      361 tgccggcgtt gcccactgag cggcgggacg acggcgatgc tgcttatctc ctgcccgtgg
      421 tgcggcgcgc gcgagcgcac cgagttccgc tgcggcggcg aggcgcacat cgtgcggccc
      481 aaggaccccg cggcgctgac ggacgagcag tgggccgact acctgttcgc caagaccaac
      541 cccaagggcc tccattacga gcgctggcac cacgcccacg gctgccggcg ctggttcaac
      601 gtcgcccgca acaccgccga cgaccgtatc ctcgcggtct accggatggg cgagccgccg
      661 cccgccattg gcggccaggc gccggcgacg ccctgcggcg agccggatct cggctcgggc
      721 aaccgggcgc tgtcgccgcc cggcccgggg gccgacacga ggtgaccggg cagcgcttcc
      781 gctgcgccga gggcgggcgc atcgaccggg cgcggccgct cgccttcacc ttcgacggcc
      841 gcgcgtacca gggcttcgcc ggcgacacgc tcgcctcggc gctggtcgcc aacggcgtgc
      901 ggctggtcgg gcgcagcttc aagtaccacc gcccgcgcgg catcttcgcc gccggcgccg
      961 acgagccgaa tgcgctggtc cggctcggcg aaggggggcg caccgaaccc aatatccgcg
     1021 ccaccgaggt cgagctttat gacggcctca tcgcatgcag ccagaaccgc tggccgtcgc
     1081 tcgcgcacga cctcggcgcg gtcatcggcc tcgccgcgcc gctggtgccg agcggcttct
     1141 actacaagac cttcatgtgg ccgccggcgc tgtggcggcg gctgtacgag ccgctgatcc
     1201 ggcgcgccgc gggcatgggc cgcgcgcccg aggcgcccga ccccgactcg tacgatcaca
     1261 tgcacggcca ttgcgacgtg ctggtcgcgg gcggcggcgc cgccgggctt gccgccgcgc
     1321 tcgccgccgg gcgcgccggc gcgcgcgtgg tcctggccga gcgcgagccc gagttcggcg
     1381 gcctgctgct gtccgagccg ccgggcgagg cgcgcatcga cggcatgccc gccatcgact
     1441 gggtcgccca agcggtcgcc gagctgagct ccctgcccga ggtgcgcctg ctgccgcgga
     1501 ccacggcgtt cggctatcac ctcgacaact atctgtgcct gctcgagcgc ctgacagatc
     1561 actttccgcc caaccaggcg ccggcggggg tgccgcgcca gcgcctgtgg acggtgcggg
     1621 cgcgacaggt ggtgctggcc gccggggcgc tcgagcggcc gttggtgttc gccggcaacg
     1681 accgcccggg ggtgatgctg gcaggcgcgg cgcggagcta cgtcaaccgg ttcgccgcca
     1741 gcccggggcg gcgcgtggtg gtgctggcga accacgacgg cggctacctg tgtgcccgcg
     1801 accttgcgcg cgccggcatc aaggtgcggg cgctgatcga cgtccgcgag cgcgcgagcg
     1861 atacgctcgc cgcgccattg cgggcggcgg ggaccgaggt gctggcgggc cacgccatcg
     1921 tcggcacggc gggccggcac cgcctgcgcg cggtgcaggt ggcgccgctc gcggccgacg
     1981 gggcgcgccg cgaaatcggc tgtgacctgt tgtgcgtctc gggcggctgg aatccggcgg
     2041 tgcacctgtt ctcgcactcg cgcgggacct tgcgcttccg cgccgacgac ggggtcttcg
     2101 tgcccgaccg ctcgcaccag gccgagcgct cggcgggtgc ctgcaacggc acctttgcgc
     2161 tttccggctg cctggccgag ggcctggccg caggccagga cgccgcacgg cgtgccgggt
     2221 tcgagtcggc ggcgatcgcg ccgccggcgg tcgaggcggc gggcgaggcg cgcgcgacgc
     2281 cgacttggct gttgcccgcc gactccaagg cgttcgtcga cgtgcagaac gacgtcaccg
     2341 ccgccgacct cggcctcgcg gtgcgcgagg gctaccgctc gatcgagcac gtcaagcgct
     2401 acaccaccac cggcatgggc accgaccagg gcaagaccgg caacgtcaat gcgctcgcca
     2461 tcgtcgccgc ggcgcgcggc gaggacatcc cggcggtcgg cgtcaccacc ttccgcccgc
     2521 cttacgcgcc ggtgagcttc ggcgcggtcg ccggccgcga ggtcgggcag ttctaccagc
     2581 cgctcaggac cacgccgatg cacgcctggc accaggccca cggcgccgcc ttcgagccgg
     2641 tcggccagtg gcaccggccc tggtactacg ctcggcccgg cgagaccatg cagcgcgcgg
     2701 tgatgcgcga ggccaaggcg gtgcgcgcgg gcgtcggcgt gctcgacgcc accaccctcg
     2761 gcaagatcga cgtccagggc cgcggcgcgg tcgagttcct caaccgcatc tacatcaacg
     2821 cctggaccaa gctcgggatc ggccgctgcc gctacggcgt gatgctcgac gaccagggca
     2881 tggtcatgga cgacggcgtc accacgcgcc tgggcgagac ccatttccac ctcaccacca
     2941 ccaccggcgg cgcggcgccg gtgctcgcct ggctcgagga atggctgcag acagaatggc
     3001 ccgagatcga ggtctacctg acctcggtca ccgagcagtg ggcggtgatc gcgctcgccg
     3061 gccccgacag ccgcaagctg ctcgcccgcc ttgccgaggg catcgacctg tcgggcgagg
     3121 cgttcccgca catggcgatg cgcgagggcg ttgttgccgg cgtggccgcg cgcgtcttcc
     3181 ggatcagctt ctcgggcgag ctcggctacg aggtcaacgt gcccgcctcg tacgggctgg
     3241 cggtgtggca ggcggtgatc gccgccggcg ccgagttcgc gctgacgccc tacggcaccg
     3301 agaccatgca cctgctgcgc gccgagaagg gctacatcat ggtcggccag gagtccgacg
     3361 gcaccgtcac cccgatcgac ctcgggcttg agcggatgat cggcaggacc aaggccgact
     3421 tcatcggc
//
LOCUS       DASWHU010000370        17777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_993713, whole genome shotgun sequence.
ACCESSION   DASWHU010000370 DASWHU010000000
VERSION     DASWHU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17777
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993713"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1007)
                     /locus_tag="VGB88_13725"
     CDS             complement(294..1007)
                     /locus_tag="VGB88_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648559.1"
                     /translation="MLEVEGLCAGYGRVPVLDHVSLAIAAGERVAVVGPNGVGKTTLL
                     KALIGLIPVESGRIEFAGAEVTREPSHRRARLGLGYVPQGRELFANLSVRDNLRMGAL
                     GGALDESQALARVLALMPRLGPLLERAAGTLSGGEQQLVAIARALAPRPRLVLLDEPS
                     EGIQPSLVEAILAVLRALADEPGAPPPALLVVEQNLDFVAALAGRALIMQRGRLVREV
                     APEALSDPEVTRALLGGGD"
     gene            complement(1010..1735)
                     /locus_tag="VGB88_13730"
     CDS             complement(1010..1735)
                     /locus_tag="VGB88_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648560.1"
                     /translation="MTAAPLLETRALSRRFGGVIALDRVDFALAERELCCLIGPNGAG
                     KSTFFKCLTGQLAPSAGAIRLRGLDIAGAPSHRIARLGIGIKTQVPSLFDGLTVLDNA
                     WLAARRRLPGRPARAAALAMLERIGVSALATRTVASLGHGERQLVELAMVLAGDPALI
                     LLDEPTAGMSRAEVERIAALIGEIARDHALIVVEHDMRFVSMIAGRVCVFHDGAVLAD
                     GALAEVLADARVQAVYLGRGGRS"
     gene            complement(1732..2748)
                     /locus_tag="VGB88_13735"
     CDS             complement(1732..2748)
                     /locus_tag="VGB88_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648561.1"
                     /translation="MAGAARLGHAALGAAALALLVALPAWAELFTVINATIWVATALL
                     ALSLGLVWGFGGILCLGQTAFFGLGGYAYAVAAINLGDTTLAVPIAVAVAAGFAAALG
                     YVLFYGRLGDVYLGVITLTVSLILFKVINSTSGPEYVIGAARLGGFNGIPATPPLNAP
                     FDPASPLSPATIFRVAMAALIAVYFGCLWLLGTGFGRVAVAIRENELRAALLGYDVRL
                     RKLALFAIGGAIAGLAGVLFANSVFVSPTMFSLYYAAQALIWVIVGGLGTLAGPALGA
                     VAVQVLTTALGRLGQLDPALARLDPNVVLGLVLVLVVLLLPQGLLPALGRALTRLAGG
                     RARP"
     gene            complement(2751..3617)
                     /locus_tag="VGB88_13740"
     CDS             complement(2751..3617)
                     /locus_tag="VGB88_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648562.1"
                     /translation="MDLAAAILIQVLVGIASLVLISIGLAVVFGMMRVINLAHGEFLM
                     LGGYAAVVSHQHGVNLWVAMLIVAPLMVGAVGVVVELVVVRRLYGRMVDTLLATWGLS
                     LLLIGLVTTVFGNVTEGVSAPLGAVRIGGFGIGQYQLVLVALAALIVLALYGGLKWTR
                     AGMIARATMQNADMAAALGVDRGRVYTLTFGLGAALSGLAGGLLAPVSGLVPTIGAAY
                     IAKAFITVISGGAAVVSGTLAAAGLLGAVNTVASFLATPVAGEVALLLAAVILLRLLP
                     QGISGRLFGGAV"
     gene            complement(3802..5034)
                     /locus_tag="VGB88_13745"
     CDS             complement(3802..5034)
                     /locus_tag="VGB88_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9648563.1"
                     /translation="MSISRRRFLAGAAGVAAAGAAAARPARAQSPIKVAGILDASGGL
                     DIYGRPMIDCMRLAVEETNAAGGLLGRPVELLVYDAQSNIQFYTQFATEAATAERVAV
                     VHGGITSASREAIRPLLRRYGTLYFYNTQYEGGVCDRNIFATGSTPAQTVARLVPHVI
                     KRWGKRIYTLAADYNYGQITARWVRKFARDAGGEVVASDFFPLDVTDFGTTISKIQAA
                     KPDMVVSVLVGGAHMSFYRQWAAAGMKDAIPLASTTFGVGNEHRVLSPAESAGIVACY
                     GYFEELETVANRAFLARLRARYGAAAPAVNELAVRSYEGFLLWADGVRRAGTTDHDAV
                     IEALESGVALDGPSGPIRIDAATHHIVQTVYLGEIKGGGFAVLDSFPDQPPADTAAVC
                     DLIANPDDTTMYEIDIDV"
     gene            5329..7320
                     /locus_tag="VGB88_13750"
     CDS             5329..7320
                     /locus_tag="VGB88_13750"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648564.1"
                     /translation="MADGPRGDSGYDELTHNMILPVVAMAAIGLVIIVFVIFLAARWQ
                     DIDAAGRSFQVASSAFNFAHRDLSNTAADYARRPDTLRHLVLEPDPDWADLNVGEPVS
                     ETHGYDWILVIGGDGSASYAWGPGGARSFAAARDGSPALERLVTLARAERARPLRPMT
                     GLAMVAGTPHVAAAVPLISDAGAAPPAVLVFARAIDEAFLDQLSRASGLSKVRLLSAA
                     GPADEDSIPLVAADGTPLGAVAWVVDQSGQEVFTKSLVLIALAGPAMIVLGWAFVRRA
                     RDTVYMLIRQERALQEERARAQEYLAVVRAVVVALDPDGRVQMINDLGRRMLGRDHSE
                     LVGRRWVALAVPEDRRAEVTGLFERMLAGADDAPATIEYEILTRDGARRLVSWQHAAI
                     RDQHGAVIGILASGDDVTDRRRMEQRVREQEAELAHFLRLGTLGEMATGLAHELNQPL
                     AAIKNFAQGCVRRMRAGNADPAELLKAIEQVNAQATRAGEIIRRMRSFVRKREPVRAP
                     MDVNQAIREVAELIARDVKRLGVSLELALDEHLPPALADMIQVEQVILNLARNGLDVI
                     ALEPASARRRLTIRSGPGEQGSVMVSVSDTGRGIAPDQVENLFEPFYTTKKEGLGMGL
                     SISRSIIEAHHGRLWAEPNPDGGSVFRFTLPAASMSKAA"
     gene            7335..7958
                     /gene="fixJ"
                     /locus_tag="VGB88_13755"
     CDS             7335..7958
                     /gene="fixJ"
                     /locus_tag="VGB88_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEX9648565.1"
                     /translation="MNDRKPTIYVVDDDEAVRNSLQWLIASIDLEVRTYGSAQEFLAD
                     FSPEAPGCLVVDVRMPGMSGLELQRQLVDRGSDIPVVVITGHGDVEMAVRAMKAGAFD
                     FIQKPFNDQVLLDLIQTALEKSTRVARAQAERTHVRDQLDQLTEREREVLNGIVAGES
                     NRQIATRLDLSEKTIEFHRAKVMRKMEAGSLAELTRKVVLAEPEPQT"
     gene            complement(8060..8251)
                     /locus_tag="VGB88_13760"
     CDS             complement(8060..8251)
                     /locus_tag="VGB88_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX9648566.1"
                     /translation="MHWLVPLRYGVRRLVKDEKGATAIEYGLIAALIAVVIIAALTMV
                     GTELNATFGKVGSELSSVN"
     gene            8813..10351
                     /locus_tag="VGB88_13765"
     CDS             8813..10351
                     /locus_tag="VGB88_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9648567.1"
                     /translation="MIGLRDRRRERPTFLERYASQLGELVERQHAEAALIAARNNAER
                     SAGDARRAQIETERANLALREEMDRRLKALADLEYLANHDPLTDLPNRNLFNLRLKQV
                     LDRARRTGEAVGLMFLDLDRFKDVNDTLGHDVGDDLLREVARRLKISVRAEDTVARVG
                     GDEFAVIQVGLNYPAEANIQVVRILRYLTEPVHIGSHKLFTSASVGITVFPRDTQSPE
                     QLQKNADLAMYLAKEDQRSTFRFYDSTLDAVAKRRAFLEQELRAAIEQEQLTVYYQPK
                     VSIRSGDPVGAEALVRWLHPEQGMVSPAEFIPVAERTGLIVPIGELTLRRACTQFQKW
                     HRDGYGQIKLAVNLSASQFSITDIPKLVREVLEETGFSPDKLELEVTETAVMNDMHNA
                     VDVLNDLHSIGVSLSIDDFGTGYSSLSYLRQLPVNRIKIDRSFIAEIDRADAAAAIAR
                     AVVNLGRSLGLEVVAEGVETVAQLEYLRSIDCDEAQGFLFSKAVPAAEFEQYLAERVK
                     PRRR"
     gene            complement(10367..12028)
                     /locus_tag="VGB88_13770"
     CDS             complement(10367..12028)
                     /locus_tag="VGB88_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9648568.1"
                     /translation="MPAVTADVATMAQTSSAELTGEPNTIGRVVSVSGGQVICLLNAP
                     EGPASRETMGLQIGSLVRLRTSASNAFGIVSGMTIPIPARDANSDEMKIVELELVGEV
                     PLRPDGKTGPFRRGMSTSPALDDAVLAATQEDLQTVYQRRSEHAVEIGRLHQNRAVPA
                     YVVVDDLLGKHFSILGTTGSGKSCAVSLLLNAIVAKHANAHIVLLDPHAEYANAFKGL
                     AECIEPDDLRLPHWLFNFEEMTEIVFGAQAPDMMTETTILRDMIQRCKVRFTGSSEES
                     RAFTVDTPTPYKFGDLLHLLEQTMGRLENRSDLTPYHRIKARLLALQSDRRYTFLFPS
                     GVVVRDNLTAILARIFRVPVAGKPISIVNLSGVPSEVLNVVVSVLCRMTFDFALWSDQ
                     RVPLLLVCEEAHRYAPARDIDGFEPTKRALARIAKEGRKYGISLGIVSQRPSEIAANV
                     LSQCNTLFAMRMTSEKDQDCLRAAMSEATGGLIDSLPSLGNGEAVAVGEGVAVPMRLV
                     FNQLEEGKMPHSGTAKFSAAWRDGDGTEESLKEVVARWRQSAASA"
     gene            12436..12909
                     /locus_tag="VGB88_13775"
     CDS             12436..12909
                     /locus_tag="VGB88_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEX9648569.1"
                     /translation="MAVDAERAPEARGEAGADAAPARGRRRRGARHALLALGWLCVAL
                     GAIGIVVPGMPTTIFLIIALWAFSRSSEQVRSWLYNHRRFGPPLQAWHAHGVIPARAK
                     LAAVAVMAASVAVLAALSHDWIGPVALAAVMVPVAAFIVSRPSAVPAGRRGIGGC"
     gene            complement(12857..13786)
                     /locus_tag="VGB88_13780"
     CDS             complement(12857..13786)
                     /locus_tag="VGB88_13780"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9648570.1"
                     /translation="MVEAAPAKVNLTLHVVGRRADGYHLLDSLIVFAGVGDVVRVRPS
                     DRLELAVAGPGAAALEAPAAGQNLVERAARALAAATGRAGGAAIELDKRLPVAAGIGG
                     GSADAAATLRALARLWRLEDGAETLRRVAAGLGADVPACLACRPVHVRGIGEVVEPLK
                     GRWPAVHLVLANPGRPLATAAVFAARAGAYGAAEPLIRVPATAAGLAGELARRRNDLE
                     APARRLMPEIGEVLDALAAAPGCLLARMSGSGATCFGLFADRARAAAAAGALARAHPA
                     WWLAAAPMLRRAPAAALSSRRSPCARPAPRSAG"
     gene            complement(13845..15686)
                     /locus_tag="VGB88_13785"
     CDS             complement(13845..15686)
                     /locus_tag="VGB88_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648571.1"
                     /translation="MDHFSGRSRASPPAGAGGGARQGALVAAAAVLILGACAGRVGEP
                     AAGPAEVTAEPATPLRLAQTSQALDLPPGAVDGEGSPLGSYLAGRHAHLENDAPSALR
                     FYLRSLEADPDNPELLNRALMGALAVRDLDLGTELARRLLEVDPESPVAALALAVDAV
                     GDGAYEDAAGWLDKMPGSGFNQYLVPLLRAWTWVGRADADQALAALEPLSANSVFAGT
                     HDYHSGLIGEIFGRSQAAENGYASALSGSPGGPLRVVQAAGGFYQRTGRLDEARAIYD
                     AFLDQNPDTTFLDAAYRALEAGAPPPLPVTSANEGFAEALYGIAISLFQENALEPALV
                     YGQLALYLRPGLDVGAMLLGDILITVGQTDAAVAAYRQVPSESGLAWSVRLRIANALA
                     SSDKIDEAAEEFRAMARERPERPDPLIQLADLMRREERYADAVTAYDQAFARIPTLEQ
                     RHWTLLYARGMSLERAHQWQRAERDLQQALELEPDQPLVLNYLGYSWVEMGIKLDAAK
                     AMIEKAAEQRPNDGYIVDSLGWVLYRLGEYERAVGHLERAAELRPMDPVILDHYGDGL
                     WRVGRKAEARFQWRRALSFQPEPELGEALDEKLSKGLGPPTEITKDM"
     gene            15869..16060
                     /locus_tag="VGB88_13790"
     CDS             15869..16060
                     /locus_tag="VGB88_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648572.1"
                     /translation="MSSRNNPARRQGRSNEKDYQNKKVKPVLYIGSFVGHGRYVAAQD
                     ESGKLVFDAYGKPIPYSQI"
     gene            16070..16879
                     /locus_tag="VGB88_13795"
     CDS             16070..16879
                     /locus_tag="VGB88_13795"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9648573.1"
                     /translation="MAEPGGRRALARRQLAWLVESGADEALGADARDRFAPAPRIQPA
                     PAPPPAAPGPSARPAPPALASTAALIERARETAEAAATVADLAREVAAFDGCALSRTA
                     KTTVFADGNPAADLMLIGEAPGAEEDRRGLPFVGAAGQLLDRMLAAIGRDRGSAYITN
                     MVYWRPPGNRNPTTEEMALCRPFVDRHIALVQPQVLVCLGAISAKSLLARNDGITRLR
                     GRWFEVQPAGLDRPVATIAIFHPAFLLRQPSQKRLAWRDLQAIRQRLTPTT"
     gene            17001..>17777
                     /locus_tag="VGB88_13800"
     CDS             17001..>17777
                     /locus_tag="VGB88_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648574.1"
                     /translation="MTGRRLIDWLAVGALVAGAIWHPPDARADDVSAGGLELVELTTP
                     SARLPRAAGSLSRVPAMLGAADISAYRRIFDLQRDGDWAAADALIAGLGNDVLLGHVL
                     RQRYMHQTWHSDYDGLCAWLGRYADHPGAMRVFRLALKRRVDGATAPAEPEGDSLRGI
                     GEISGRAGPALPRLKLDPVQRQAGDALLERVQHLVGDGKPGEALDALAQPEAGSLLSA
                     AEYDAARATVARGLFSAGRDREAYDVARHAAARSGRLVGDA"
BASE COUNT         2649 a         6456 c         6287 g         2385 t
ORIGIN      
        1 actcgccggg catcaggtag cggtcgtcga gcaccgcctt gccgaacgcg gtcagcagcc
       61 ggtcgcggct cgggtcggtg cgcacccgca cgccgcgctc gagctgcacc acgttcagca
      121 tggcgtcgct gtgtcgcacc ccctcggccc ccgctctcca caggctgtgg ataatttcct
      181 gtggataaaa tcaacatctt ggatgtcgat tggcggaaga ctaccagatg tatggaaaca
      241 cgcaagcccc tatttgcgtc cccgaaaggt tctttggccc cggcgggcgg cagtcaatcg
      301 ccgccgccga ggagcgcgcg ggtgacctcg gggtccgaca gcgcctcggg cgccacctcg
      361 cgcaccagcc ggccgcgctg catgatcagg gcgcgcccgg cgagcgcggc gacgaagtcg
      421 aggttctgct cgaccaccag cagcgccggc ggcggcgcgc ccggctcgtc ggcgagcgcg
      481 cgcagcaccg ccaggatggc ctcgaccagg gacggctgga tgccttccga cggctcgtcg
      541 agcagcacca gccgcggccg gggggcgaga gcgcgcgcga tcgccaccag ctgctgctcg
      601 ccgccgctga gcgtgccggc cgcgcgctcg aggagcggcc cgagccgcgg catgagcgcg
      661 agcacgcgcg ccagcgcttg cgattcgtcc agcgcgcccc cgagcgcgcc catgcgcagg
      721 ttgtcgcgca cgctcaagtt ggcgaacagc tcgcgcccct ggggcacgta gccgagcccg
      781 agccgcgcgc gccggtggct cggctcgcgg gtcacctccg cgccggcgaa ctcgatgcgc
      841 ccggactcga ccgggatcag cccgatcagc gcctttaaga gcgtggtctt gccgaccccg
      901 ttggggccga ccaccgccac ccgctcgccc gccgcgatgg cgaggctgac atggtcgagc
      961 acgggcaccc ggccatagcc cgcgcacaag ccctcgacct cgagcacgct cagctcctgc
     1021 ctccgcggcc gagatagacc gcctgcacgc gcgcgtccgc cagcacctcg gcgagcgcgc
     1081 cgtcggcgag caccgcgccg tcgtggaaca cgcatacccg cccggcgatc atggacacga
     1141 agcgcatgtc gtgctcgacc acgatcagcg cgtggtcgcg ggcgatctcg ccgatcagcg
     1201 cggcgatccg ctcgacctcg gcccggctca tgccggcggt cggctcatcg agcaggatca
     1261 gcgccggatc gccggccagc accatagcga gctcgaccaa ctggcgctcg ccgtggccga
     1321 ggctggccac cgtgcgcgtc gccaaagcgg aaacgccgat gcgctcgagc atcgccagcg
     1381 cggcggcgcg cgcgggccgg ccggggagcc gccggcgcgc cgcgagccag gcgttgtcga
     1441 gcacggtgag gccgtcgaac aggctcggca cctgggtctt gatgccgatg ccgagccggg
     1501 cgatgcggtg gctgggcgcg ccggcgatat cgaggccgcg gaggcggatc gcgcccgcgc
     1561 tcggcgcgag ctggccggtc aggcacttga agaaggtgct cttgccggcg ccgttgggcc
     1621 cgatcaggca gcacagctcg cgctcggcca acgcgaagtc gacgcgatcg agcgcgatca
     1681 cgccgccgaa ccgccggctg agcgcgcgcg tctccagcag cggcgccgcg gtcacggccg
     1741 cgcccgcccg ccggcgagcc gcgtcagcgc acgcccgagc gccggcaaca gcccctgcgg
     1801 cagcagcagc acgaccagca ccagcaccag cccgagcacg acgttgggat cgaggcgggc
     1861 gagcgccggg tcgagctggc cgagccggcc cagcgccgtg gtcaggacct gcaccgcgac
     1921 cgcgcccagc gccggcccgg cgagcgtgcc gaggccgccg acgatcaccc agatcagcgc
     1981 ctgggcggca tagtacaggc tgaacatggt cgggctgacg aacaccgagt tggcgaacag
     2041 cacgcccgcc agcccggcga tggcgccgcc gatcgcgaac agggcgagct tgcgcaggcg
     2101 cacgtcgtag ccgagcagcg ccgcgcgcag ctcgttctcg cggatcgcca ccgcaacccg
     2161 gccgaagccc gtgccgagca gccacaggca gccgaagtag accgcgatca gggccgccat
     2221 cgccacccgg aagatggtcg ccggggaaag cggcgaggcc ggatcgaacg gcgcgttcaa
     2281 cggcggcgtc gccgggatgc cgttgaagcc gccgaggcgc gcggcaccga tcacatattc
     2341 ggggccggag gtgctgttga tcaccttgaa caggatcagc gaaacggtga gcgtgatgac
     2401 gccgagatag acatcgccca ggcggccgta gaacaggacg tagccgagcg cggcggcgaa
     2461 gcccgccgcc accgcgaccg cgatcggcac cgcgagcgtg gtgtcgccga ggttgatcgc
     2521 ggccaccgca taggcgtagc cgccgaggcc gaagaacgcg gtctggccca ggcacaggat
     2581 gccgccgaat ccccacacca ggccgaggct gagggcgagc agcgcggtcg ccacccagat
     2641 ggtcgcgttg atgacggtga acagctcggc ccatgccggg agcgcgacca agagcgcgag
     2701 cgccgccgcg ccgagggcgg cgtggccgag gcgcgcggcc cccgccatgc tcacaccgcc
     2761 ccgccgaaca ggcgccctga gatgccttgg ggcaagaggc gaagcaggat cacggcggcg
     2821 agcagcagcg ccacctcgcc cgccaccggc gtcgccagga agctcgccac ggtgttgacc
     2881 gcgcccagca ggccggcagc ggcgagcgtt cccgacacca ccgccgcacc gccgctgatc
     2941 acggtgatga acgccttggc gatgtaggcc gcgccgatgg tcggcaccag tcccgagacc
     3001 ggcgccaaga ggccgccggc gagcccgctc aaggcggcgc cgaggccgaa ggtaagcgtg
     3061 tagacgcggc cgcggtcgac gccgagcgcc gccgccatgt ccgcgttctg catggtcgcg
     3121 cgcgcgatca tgccggcgcg ggtccatttg agcccgccat agagcgcgag cacgatcagc
     3181 gccgccagcg ccaccagcac cagctggtac tggccgatgc cgaagccgcc gatccggacc
     3241 gcccccagcg gcgccgacac cccctcggtg acgttgccga agacggtcgt caccaggccg
     3301 atcagcagca ggctcagccc ccaggtggcg agcagggtgt cgaccatgcg gccatagagg
     3361 cggcggacca cgaccaattc gaccaccacc ccgaccgcac cgaccatcag cggcgcaacg
     3421 atgagcatgg caacccacag gttgacgccg tgctggtggg aaaccaccgc ggcatagccg
     3481 ccgagcatca ggaactcgcc atgcgccagg ttgatcaccc gcatcatgcc gaacaccacc
     3541 gccagcccga tgctgatcag caccaggctg gcgatgccga ccaggacctg gatcaggatc
     3601 gccgccgcga ggtccatcgc tccccccttg cccgagcgcg cgggacctca gcgatcgtgg
     3661 agcttttccc gcacccttcg cgacgcgccc tgcgggcgct cctcagggtg aggtgacgct
     3721 ttcgatacaa atatcacctc gtcctgagga ggccgcgaag cggccgtctc gaaggacgac
     3781 atgcgacaca ttcacgcgcc ctcagacgtc gatgtcgatc tcgtacatgg tggtgtcgtc
     3841 gggattggcg atcaggtcgc agaccgcggc ggtgtcggcg ggcggctggt cggggaagct
     3901 gtcgagcacc gcgaagcccc cgcccttgat ctcgcccaga tagacggtct ggacgatgtg
     3961 atgggtcgcc gcatcgatcc gtatggggcc gctcgggccg tcgagggcga cgcccgactc
     4021 gagcgcctcg atgaccgcgt cgtggtcggt ggtgccggcc cgccgcaccc cgtccgccca
     4081 cagcaggaag ccctcgtagg agcgcaccgc gagctcgttc accgccggcg ccgccgcgcc
     4141 gtagcgcgcc ctgaggcggg cgaggaaggc ccggttggcg accgtctcga gctcctcgaa
     4201 atagccgtag caggcgacga ttccggcgct ctcggccggc gacagcacgc ggtgctcgtt
     4261 gcccaccccg aaggtggtcg aggcgagcgg gatcgcgtcc ttcatgcccg ccgccgccca
     4321 ctggcggtag aatgacatgt gcgcgccgcc gaccagcacc gagaccacca tgtccggctt
     4381 ggccgcctgg atcttgctga tggtggtgcc gaagtcggtg acatcgagcg ggaagaagtc
     4441 gctggcgacc acctcgccgc cggcgtcgcg cgcgaacttg cgcacccagc gcgcggtgat
     4501 ctggccgtaa ttgtagtcgg cggcgagcgt gtagatgcgc ttgccccagc gcttgatcac
     4561 gtgcggcacc agcctggcca cggtctgcgc aggcgtcgag ccggtggcga agatgttgcg
     4621 gtcgcacacg cccccttcgt attgggtgtt gtagaagtag agcgtgccgt agcgcctgag
     4681 cagcggccgg atcgcctcgc gcgaggcgct ggtgatgccg ccatggacta ccgccacgcg
     4741 ctcggccgtc gccgcctcgg tcgcgaactg ggtgtagaac tggatgttcg actgggcgtc
     4801 gtagaccagc agctcgaccg ggcgcccgag caggccgccg gcggcattgg tctcttcgac
     4861 cgcgagccgc atgcagtcga tcatcggccg gccgtagatg tcgagaccgc ccgaggcgtc
     4921 gaggatgccg gcgaccttga tcgggctttg ggcgcgcgcc gggcgcgccg cggccgcacc
     4981 cgcggcggcc acgccggcgg caccggcaag gaagcgcctg cgcgaaatcg acatcgttgc
     5041 cgtctccaga taccggctgg accgagccgg gcatgccgcc ggcaacgcta gtgacggccg
     5101 ctccgtgagt caacgcgccg cgaccgtcgc ggccgctccg gcgcgccgaa gggtcgttgt
     5161 cgggctcacg atcgagcggg cgaaacggcg gccggcggcc cggcgcggcc gtccccggcc
     5221 gggcgctcgc gacccctgcc cctggcccgc acccccgtcg cgccttattt gctaagatgc
     5281 cgctcgccgg cggccggcat cgggcggcgc gagaggaggt tgccagccat ggcggacggc
     5341 ccgcgtggcg acagcggcta tgacgagctg acgcacaaca tgatcctgcc ggtggtcgcg
     5401 atggcggcta tcggcctggt gatcatcgtg ttcgtcatct tcctggccgc gcgctggcag
     5461 gacatcgacg ctgccgggcg ctcgttccag gtcgcctcca gcgccttcaa cttcgcccac
     5521 cgcgacctgt cgaataccgc cgccgactat gcccgccggc ccgatacgct gcgccacctg
     5581 gtgctcgagc ccgacccgga ctgggccgac ctcaatgtcg gcgagccggt gtcggagacc
     5641 cacggctacg actggatcct ggtgatcggc ggcgacggca gcgcgagcta cgcctggggg
     5701 ccgggcgggg cgcgcagctt cgcggcggcc cgggacggct cgccggcgct cgagcggctg
     5761 gtgacgctgg cgcgcgcgga gcgtgcccgc ccgctcaggc cgatgaccgg cctggcgatg
     5821 gtcgccggca cgccgcatgt cgccgccgcg gtgcccctga tctccgatgc cggcgccgcg
     5881 ccgccggcgg tgctggtgtt cgcgcgcgcg atcgacgagg cgttcctcga ccagctgtcg
     5941 cgggcctcgg gcctgtcgaa ggtgcggctt ctgtccgccg ccggcccggc cgacgaggac
     6001 tcgattccgc tggtcgccgc cgacggcacg ccgctcggcg ccgtcgcctg ggtggtcgac
     6061 cagtccggcc aggaggtgtt caccaagtcg ctggtcctga tcgcgctcgc cgggccggcc
     6121 atgatcgtgc tcggctgggc gttcgtgcgc cgcgcccgcg acaccgtgta catgctgatc
     6181 cgccaggagc gcgccttgca ggaggaacgc gcgcgcgccc aggaatacct tgccgtggtg
     6241 cgcgcggtgg tggtcgcgct cgacccggac gggcgcgtgc agatgatcaa cgacctcggc
     6301 cggcggatgc tcggccgcga ccactccgag ctggtcgggc gacgctgggt cgcgctcgcc
     6361 gtgcccgagg accgccgggc cgaggtgacc ggcctgttcg agcgcatgct cgccggcgct
     6421 gacgacgcgc cggcgaccat cgagtacgag attctcactc gcgacggcgc ccggcgcctg
     6481 gtgtcgtggc agcacgccgc gatccgcgac cagcacggcg cggtgatcgg catcctggcc
     6541 agcggcgacg acgtcaccga ccgccggcgc atggagcaga gggtgcgcga gcaggaggcg
     6601 gagcttgccc atttcctgcg cctgggcacg ctcggcgaga tggccaccgg gctcgcccac
     6661 gagctcaacc agccgctcgc cgcgatcaag aacttcgccc agggctgcgt gcggcggatg
     6721 cgcgcgggca acgccgatcc ggccgagctg ctcaaggcga tcgagcaggt caatgcccag
     6781 gcgacccgcg ccggcgagat catccggcgc atgcgcagct tcgtgcgcaa gcgcgagccg
     6841 gtgcgcgcgc cgatggacgt caatcaggcg atccgcgagg tcgccgagct gatcgcgcgc
     6901 gacgtcaagc gcctcggcgt cagcctggag ctggcgctcg acgagcacct gccgcccgcg
     6961 ctcgccgaca tgatccaggt cgagcaggtg atcctcaacc tggcgcgcaa cggcctcgat
     7021 gtgatcgcgc tcgagccggc ctccgcccgg cgccggctga ccatccgctc gggcccgggc
     7081 gagcagggct cggtgatggt ctcggtcagc gataccggcc gcggcatcgc gccggaccag
     7141 gtcgagaacc tgttcgagcc gttttacacc accaagaagg aggggctggg aatgggcctg
     7201 tcgatctcgc gctcgatcat cgaggcgcat cacggccggc tgtgggccga gcccaacccc
     7261 gacggcggca gcgtattccg attcacgctg ccggccgcta gcatgtccaa agccgcgtga
     7321 acgggatggc gatcatgaat gaccggaaac cgacgatcta tgtcgtcgac gacgacgagg
     7381 cggtacgcaa ctcgctgcaa tggctgatcg cctcgatcga cctggaggtg cgcacctacg
     7441 gctcggcgca ggagttcctg gccgacttct cgcccgaggc gccgggctgc ctggtggtcg
     7501 acgtgcgcat gcccggcatg agcggcctgg agctgcagcg ccagctggtc gatcgcggca
     7561 gcgacattcc ggtggtcgtg atcaccggcc acggcgacgt cgagatggcg gtgcgcgcca
     7621 tgaaggcggg cgccttcgac ttcatccaga agccgttcaa cgaccaggtg ctgctcgacc
     7681 tgatccagac cgcgcttgag aagagcacgc gcgtcgcccg cgcgcaggcc gagcggaccc
     7741 acgtccgcga ccagctggat cagctgaccg agcgcgagcg cgaggtgctg aacggcatcg
     7801 tcgccggcga atcgaatcgc cagatcgcga cccggctgga cctgagcgaa aagacgatcg
     7861 agttccatcg cgccaaggtg atgcgcaaga tggaggccgg gtcgctggcc gagctgaccc
     7921 gcaaggtggt gctggccgag cccgagccgc agacctgaga gcgcgctagc ggcggggcga
     7981 aacgcaacgc ccccggccac ggcgggccgg gggcgaagcg ggtgagaaca aacgtttgct
     8041 ccgaaccaac cgcacgcggt tagttgaccg aggagagttc gctgccgacc ttgccgaagg
     8101 tggcattgag ctcggtgccg accatggtca gcgcggcaat gatcaccacc gcgatcagcg
     8161 cggcaatcag gccgtactcg atggcggtcg cgcctttctc gtccttgacg agacgacgaa
     8221 cgccgtaacg gagcggaacg agccagtgca tcatcaattc ctccaaattt gccctagtca
     8281 cagtcgccat gaaccaaacg gaccggttta acctcgtgca tatgagaccg atcggccgcg
     8341 cacgtagtga ctttcgtcac caagtcccca actcctcggc cccccggacg cagatgtttg
     8401 tcccgttttc cacgacccgg cccaatcttg ccgctcgcac gtgaaccgcc cccggcaact
     8461 gcacggagtg ggtgagaatg gccagcgggc ctcctagagc atctagggtt atcccgaggg
     8521 ttgtcaatat ccaacatcga ggtcgatttc ttttcttttc ggcgctgtcc ggcgaccgcc
     8581 tgcgaaaggc gaatctagcg cggccgaggg attggtcaac agcggccccg ggaagtgccc
     8641 caacccgacc ggtcgcgtcc ctcgcccggg ccggcattag gcataatctc ctaggtaccg
     8701 ggaagcgggc cccgcgggtg ccacgccccg gggcgagggc gcggcggcgg cgtcggcccg
     8761 gcgaggggtg gtgccggtgc cgcgggggga acgacggaga gggcggcagc gcatgatcgg
     8821 actccgcgac cggcggcggg agcgcccaac ctttctcgaa cgctacgcca gccagctcgg
     8881 cgagctggtc gagcgccagc acgccgaggc ggccctgatc gcggcccgga acaacgccga
     8941 gcggtccgcg ggcgatgcgc gccgggccca gatcgagacc gagcgcgcca acctggcgct
     9001 gcgcgaggag atggaccggc ggctgaaggc gctggccgat ctcgaatacc tggccaatca
     9061 cgacccgctg accgatctgc ccaaccgcaa cctgttcaac ctgcgcctga agcaggtgct
     9121 cgaccgcgcc cggcgcaccg gcgaggcggt cgggctgatg ttcctcgacc tcgaccggtt
     9181 caaggacgtc aacgacaccc tcggccacga cgtcggtgac gacctgctgc gcgaggtggc
     9241 gcgccggctc aagatctcgg tgcgcgccga ggacacggtc gcgcgcgtcg gcggcgacga
     9301 gtttgcggtc atccaggtcg gcctcaacta cccggccgag gccaacatcc aggtcgtgcg
     9361 catccttcgc tacctgaccg agccggtcca tatcggctcc cacaagctgt tcaccagcgc
     9421 ctcggtggga atcaccgtct ttccccgcga cacccagtcc cccgagcagc tgcagaagaa
     9481 cgccgaccta gccatgtacc tggccaagga ggaccagcgg agcacgttcc ggttctacga
     9541 ttcgaccctc gatgcggtgg ccaagcggcg cgcgttcctc gagcaggagc tgcgcgcggc
     9601 gatcgagcag gagcagctga cggtctacta ccagcccaag gtgtcgatcc gcagcggcga
     9661 tccggtcggc gccgaggcgc tggtccgctg gctgcacccc gagcagggca tggtgtcgcc
     9721 ggcggagttc atcccggtcg ccgagcgcac cgggctgatc gtgccgatcg gcgagttgac
     9781 gctgcggcgg gcgtgcacgc agttccagaa atggcaccgg gacggctatg gccagatcaa
     9841 gctggcggtc aacctgtcgg cgtcgcagtt cagcatcacc gacatcccga agctggtgcg
     9901 cgaggtgctg gaggagaccg gcttcagtcc cgacaagctc gagctcgagg tcaccgagac
     9961 ggcggtgatg aacgacatgc acaacgccgt cgacgtgctc aacgacctgc attcgatcgg
    10021 cgtcagcctg tcgatcgacg acttcggcac cggctactcg tcgctcagct atctgcgcca
    10081 gctgccggtc aaccggatca agatcgaccg ctcgttcatc gccgagatcg accgcgccga
    10141 cgccgccgcc gcgatcgcgc gcgcggtggt caatctgggc cggagcctgg ggctcgaggt
    10201 ggtcgccgag ggggtcgaga cggtggccca gctcgagtac ctgcgcagca tcgactgcga
    10261 cgaggcgcaa ggcttcctgt ttagcaaggc ggtgccggcg gccgagttcg agcagtacct
    10321 ggccgaacgc gtcaagccgc ggcgcaggta gccgcccggc gtccgctcag gcgctggcgg
    10381 cggactggcg ccagcgcgcg acgacttcct tgagcgattc ctccgtgccg tcgccgtcgc
    10441 gccaggcggc cgagaacttg gcggtgccgc tgtgcggcat ctttccctct tcaagctggt
    10501 tgaacaccag gcgcatcggc accgccaccc cctcgcccac cgccaccgcc tcgccgttgc
    10561 cgagcgaggg cagcgagtcg atcaggccgc cggtcgcctc cgacatcgcc gcgcgcaggc
    10621 aatcctggtc cttctcgctg gtcatgcgca tggcgaacag cgtgttgcac tgggacagca
    10681 cgttggccgc gatctccgac ggccgctggc tgacgatgcc cagcgagatg ccgtacttgc
    10741 gcccttcctt ggcgatccgg gcgagcgccc gcttggtcgg ctcgaatccg tcgatgtcgc
    10801 gcgccggcgc atagcggtgg gcctcctcgc agaccagcag caagggcacc cgctgatcgc
    10861 tccatagggc gaagtcgaag gtcatccggc acagcaccga aacgaccacg ttgagcacct
    10921 cggacggcac ccccgacagg ttgacgatcg agatcggctt gccggcgacc ggcacccgga
    10981 agatgcgcgc caggatggcg gtcaggttgt cgcgcaccac cacccccgag gggaacaaga
    11041 acgtgtaccg gcggtcggac tgcagcgcca gcagccgggc cttgatccga tggtacgggg
    11101 tcaggtcgct gcggttctcg agccgcccca tggtctgttc cagaagatgc agcaggtcgc
    11161 cgaacttgta cggcgtcggg gtatcgacgg tgaacgcgcg cgattcctcg gagctgccgg
    11221 tgaagcgcac cttgcacctc tggatcatgt cgcgaaggat ggtggtctcg gtcatcatgt
    11281 ccggcgcctg ggcgccgaat acgatctcgg tcatctcctc gaagttgaac agccagtgcg
    11341 gcaggcgcag gtcgtcgggc tcgatgcact cggccaggcc cttgaaggcg ttcgcatact
    11401 cggcgtgagg atcgagcagc acgatgtgcg cgttggcgtg cttggcgacg atcgcgttca
    11461 gcagcagcga caccgcgcag ctcttgcccg agccggtggt gccgagaatc gagaaatgct
    11521 tgcccagcag gtcgtcgacc acgacatagg ccggcaccgc gcggttctgg tgcagccgcc
    11581 cgatctcgac cgcgtgctcg gagcggcgct ggtagaccgt ctgcaggtcc tcttgggtcg
    11641 ccgccagcac cgcgtcgtcg agcgcgggcg aggtcgacat gccgcggcgg aacggcccgg
    11701 tcttgccgtc ggggcgcagc ggcacctcgc cgaccagctc gagctcgacg atcttcatct
    11761 cgtcgctgtt cgcatcgcgc gccgggatcg ggatggtcat gccgctgacg atgccgaagg
    11821 cgttgctcgc cgacgtgcgc agcctgacca gcgagccgat ctgcagcccc atcgtctcgc
    11881 ggctcgccgg cccctcgggc gcgttcaaca ggcagatcac ctggccgccc gaaaccgata
    11941 ccacccggcc gatggtgttg ggctcgcccg tcagctccgc gctcgacgtc tgcgccatcg
    12001 tggcgacgtc ggcagtcacc gccggcatgt tcatcgcccg cggcgcccgt gcgagcgcgc
    12061 cgtgccggcg gcggcgccgc cttcgtcgat ccaacgatcg atgcgccatc gaagcgacat
    12121 cgcggcgtcc tcgcaccaaa ccctgctgtc gtcgccaacg gcaccgggcc gcagcacgcg
    12181 cgacccggca ccgcgccttc ccgggcggag cgcagcctac ggcttgccgg cacggggcgg
    12241 cgcgcctcgg ttgcaaaggc tggttagaaa ataaggcccg tccggcaacc ggcaatccga
    12301 atcgttccgc cggcgcgcgc gtcgaattgc ttgcgcctcg ggcagtgccg gggaccgcgc
    12361 gctcgcttgc cggcccgggc gggcgcgcct accatcgcca tggttggcca ccgccagtgg
    12421 aggacgagca gggtcatggc cgtcgacgcc gagcgcgcgc ccgaggcgcg cggcgaagcc
    12481 ggcgcggatg ctgcgcccgc ccgcggccgg cgccggcgcg gcgcccgcca cgcgctgctc
    12541 gcgctcggct ggctgtgcgt cgcgctcggc gccatcggca tcgtggttcc gggcatgccg
    12601 accacgatct tcctgatcat cgccctgtgg gcgttctcac ggagctcgga gcaggtccgt
    12661 tcgtggctgt acaatcaccg ccgcttcggc ccgccgctgc aggcctggca cgcccacggc
    12721 gtcatcccgg cgcgcgccaa gcttgccgcg gtggcggtga tggcggcgag cgtcgcggtg
    12781 ctcgccgccc tctcccacga ctggatcggc cccgtggccc tggccgcggt gatggtgccg
    12841 gtggcggcgt tcatcgtcag ccggccgagc gcggtgccgg ccgggcgcag ggggatcggc
    12901 ggctgctgag cgcggcggcg ggcgcgcgcc tgagcatcgg cgccgctgcc agccaccatg
    12961 ccggatgggc gcgggcgagc gcgccggcgg cggcggccgc ccgggcgcgg tcggcgaaca
    13021 gcccgaagca ggtcgcgccg ctgcccgaca tgcgcgcgag caggcatccc ggcgccgccg
    13081 ccagcgcgtc gagcacctcg ccgatctcgg gcatcaggcg ccgcgccggc gcctcgaggt
    13141 cgttgcgccg ccgcgccagc tcccccgcca gcccggccgc cgtggccggc acccggatca
    13201 gcggctccgc cgccccgtag gcgccggcgc gggccgcgaa caccgccgcc gtggccagcg
    13261 gccggccggg gttggcgagc accagatgga ccgccggcca gcggcctttc agcggctcga
    13321 cgacctcgcc gatgccgcgc acatggaccg gccggcaggc gaggcatgcg ggcacgtcgg
    13381 cgcccagccc ggcggcgacg cgccggagcg tctcggcgcc gtcctcgagc cgccacaggc
    13441 gggcgagcgc gcgtaaggtc gcggcggcgt ccgccgagcc gccgccgatg ccggcggcga
    13501 ccggcaggcg cttgtcgagc tcgatcgcgg cgccgccggc gcggccggtc gcggcggcaa
    13561 gcgcgcgcgc cgcgcgctcg accaggttct ggcccgccgc cggcgcctcg agcgccgccg
    13621 ccccgggccc ggcgacggcg agctcgagcc ggtcggaggg gcgcacgcgc accacgtcgc
    13681 cgacaccggc gaacacgatc aggctgtcga gcaggtggta gccgtcggcg cgccggccga
    13741 ccacgtgcag ggtcaggttg accttggccg gcgccgcctc gaccacctcg ggcgcaggcg
    13801 cgcgccggtc cgccccggcc cgtgccggcg acgagcgagc gatgtcacat gtccttggta
    13861 atctcggtcg gcgggccgag gcctttgctg agcttctcgt caagcgcctc gccaagctcc
    13921 ggctcgggct ggaacgacaa cgcccggcgc cactggaagc gcgcctcggc cttgcgtccc
    13981 acccgccaca ggccgtcgcc gtagtggtcg aggatgaccg ggtccatcgg cctcagctcg
    14041 gccgcccgct cgagatggcc gaccgcccgc tcgtactcgc cgagccgata gagcacccag
    14101 cccaggctgt cgacgatgta gccgtcattg gggcgctgct cggcggcctt ctcgatcatc
    14161 gccttggccg cgtccagctt gatgcccatc tcgacccagc tgtagccgag atagttgagc
    14221 accagcggct ggtccggctc gagctcgagc gcctgctgca ggtcgcgctc ggcgcgctgc
    14281 cactggtgcg cccgctcgag cgacatgccg cgggcgtaga gcagcgtcca gtgccgctgc
    14341 tccagcgtcg gaatgcgcgc aaatgcctgg tcgtaggcgg tgaccgcgtc cgcgtagcgc
    14401 tcctcgcgcc gcatcaggtc ggcgagctgg atcaacgggt cgggccgctc ggggcgctcg
    14461 cgcgccatcg cccggaactc ctcggcggcc tcgtcgatct tgtccgacga ggcgagcgcg
    14521 ttggcgatgc gcaggcgcac cgaccaggcc aggcccgact cgctcggcac ctggcgatag
    14581 gccgccaccg cagcgtcggt ctggccgacc gtgatcagga tgtcgccgag cagcatcgcg
    14641 ccgacgtcga ggccgggacg caggtaaagg gccaactggc catagaccag cgccggctcg
    14701 agcgcgttct cctggaacag cgagatggcg atgccgtaca gcgcctcggc gaagccctcg
    14761 ttcgcgctcg tcaccggcaa cggcggcggc gcgccggcct cgagcgcgcg gtaggcggcg
    14821 tcgaggaagg tggtatcggg gttctggtcg aggaacgcgt cgtagatcgc gcgcgcctcg
    14881 tccagccggc cggtgcgctg gtagaacccg cccgccgcct ggaccacgcg caacgggccg
    14941 cccggcgagc cgctgagcgc gctggcatag ccgttctccg cggcctggct gcggccgaag
    15001 atctcaccga tcaggccgct gtggtagtcg tgggtgccgg cgaacaccga gttggccgac
    15061 agcggctcga gcgcggccag cgcctggtcg gcgtcggcgc gcccgaccca ggtccaggcc
    15121 ctgagcagcg gcaccaggta ctggttgaag cccgagcccg gcatcttgtc gagccacccg
    15181 gcggcgtcct cgtaggcgcc gtcgccgacc gcgtccaccg ccagtgccag cgccgccacc
    15241 ggcgactcgg gatcgacctc gagcaggcgc cgggcgagct cggtcccgag gtccaggtcg
    15301 cgcacggcga gcgcgcccat cagcgcccgg ttgagcagct ccggattgtc gggatccgct
    15361 tccagcgaac gcaagtagaa cctgagcgcg ctgggggcgt cgttctcgag atgcgcgtgg
    15421 cggccggcca gatagctgcc gagcggcgag ccttcgccgt cgaccgcgcc cggcggcaga
    15481 tccagcgctt gcgaggtctg cgccaggcgc agcggggtcg ccggctcggc ggtaacctcg
    15541 gccgggccgg ccgcgggttc gccgacgcgc cccgcgcagg cgccgaggat cagcaccgcc
    15601 gccgccgcga ccagcgcccc ctggcgggca ccgccgccgg cgcccgccgg aggcgacgcc
    15661 cgagaccttc cgctaaagtg gtccactgcc atctcgaccg cgcctacagg ttgcccgccc
    15721 aggactgagt ctattcgccg ccgccgcact tgcaatccac accttgctcc gggcgcgcgc
    15781 ggcggttgac ctttcgcccc gcttcgcgca caacggcgcc ccggggacag acacgaataa
    15841 gtatagcgtt gattgaaggg tggcacgcat gtccagccga aacaatcccg cccgccgcca
    15901 gggccggtcc aacgagaagg actaccagaa caagaaggtc aagccggtgc tctacatcgg
    15961 ctcgttcgtc ggccacggcc gctatgtcgc cgcccaggac gagtccggca agctggtctt
    16021 tgacgcctac ggcaagccga tcccctacag ccagatctga gcggcggcgg tggcggagcc
    16081 cggcggccgg cgcgcgcttg cccgccgcca gctcgcctgg ctggtcgaga gcggcgccga
    16141 cgaggcgctc ggcgctgacg cccgcgaccg gttcgcgccc gcaccgcgca tccagccggc
    16201 gccggccccg ccgcctgcgg cgcccgggcc gagcgcgcgc ccggcgccgc ccgcgctcgc
    16261 cagcaccgcg gcgctgatcg agcgggcgcg ggaaaccgcc gaggcggcag cgacggtcgc
    16321 ggacctcgcc cgggaggtcg ccgcgttcga cggctgcgcg ctcagccgga ccgccaagac
    16381 caccgtgttc gccgacggca acccggccgc cgacctgatg ctgatcggcg aggcgccggg
    16441 cgccgaggag gaccgccgcg gcctgccctt cgtcggcgcc gccggccagt tgctcgaccg
    16501 catgctggcc gcgatcgggc gcgatcgggg ctccgcctac atcaccaaca tggtgtactg
    16561 gcggccgccg ggtaaccgca acccgacgac cgaggagatg gcgctctgcc gcccgttcgt
    16621 cgaccgccac atcgccctgg tgcaacccca ggtgctggtc tgcctgggcg ccatctcggc
    16681 caagagcctg ctcgcccgca acgacggcat cacccgcctg cgcgggcgct ggttcgaggt
    16741 ccagcccgcc gggctcgacc gcccggtcgc caccatcgca atcttccacc cggccttcct
    16801 gctgcgtcag ccgagccaga agcggctcgc ctggcgcgac ctgcaggcga ttcgccagcg
    16861 cctgaccccc acaacctgaa gtagtagcag cgaaatcggc cctaccggcg ggactcgtgc
    16921 gggttgcgcc gggcgcccga attccgtata agacatgcct atttttagtc gcattttaga
    16981 caaatatggg cgtcgggggg atgacaggac ggaggctcat cgattggctc gcggtggggg
    17041 cgctggtcgc cggcgcgatc tggcatccgc ccgacgcgcg cgcggacgac gtgagcgcgg
    17101 gcgggctgga gctggttgag ctgaccacgc cgtccgcccg gctgccgcgc gccgcgggca
    17161 gcttgagccg cgtcccggcg atgctcggcg ccgccgacat ctccgcctat cggcgcatct
    17221 tcgacctgca gcgcgacggc gactgggcgg cggcggacgc gctgatcgcc gggctcggca
    17281 acgacgtgct gctcggccat gtgctgcgcc agcgctacat gcaccagact tggcactcgg
    17341 actacgacgg gctgtgcgcc tggctcgggc gctacgccga ccatccgggc gcgatgcgcg
    17401 tgttccgcct ggcgctcaag cggcgcgtcg acggcgcgac ggcgccggcg gagccggagg
    17461 gcgactcctt gcgcggcatc ggcgagatct cgggccgcgc cggaccggcg ctgccgcgcc
    17521 tcaagctcga ccccgtccag aggcaagcgg gcgatgcgct gctggagcgc gttcagcacc
    17581 tggtcggcga cggcaagccg ggcgaggcgc tggatgccct cgcccaaccc gaggccggat
    17641 cgctgctgag cgcggcggag tatgacgcgg cgcgcgcgac cgtcgcccgc ggcctgttct
    17701 ccgccggacg cgaccgcgag gcctacgacg tcgcccgcca tgccgcggcg cgctcgggaa
    17761 ggctggtggg cgatgcc
//
LOCUS       DASWHU010000371         9347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1748569, whole genome shotgun sequence.
ACCESSION   DASWHU010000371 DASWHU010000000
VERSION     DASWHU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748569"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VGB88_13805"
     CDS             <1..603
                     /locus_tag="VGB88_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine nucleotide alpha hydrolase"
                     /protein_id="HEX9648575.1"
                     /translation="GWALSVVEAGEFADPAYLANPHDRCFHCKTNLYRTIGALTARTV
                     VSGANLDDLADYRPGLAAAARHGVRHPYVEANIDKACVRALAAGLGLDEVADLPAAPC
                     LSSRVETGIAIDADMLRLIDAAERFVRRRVRPDTVRCRLRGSGVVIELDEPALARLEG
                     GAAASLAEAVAAMFARAGARRAVRVAPYVRGSAFVGGARP"
     gene            600..1304
                     /gene="larB"
                     /locus_tag="VGB88_13810"
     CDS             600..1304
                     /gene="larB"
                     /locus_tag="VGB88_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX9648576.1"
                     /translation="MNDVAREVRLDFGRGPRIGLDEAILCEHKSLEQIVHIMEQARVR
                     ARSLLLTRLGADLAAALPEQLAAALDYCATSRTAFFGSVAAPSAPARVAVVAGGSSDA
                     PVAREAARTLAFAGQASELAFDVGVAGLWRLLDEIGRLERMAVVIVAAGMDAALVSVV
                     AGLVPGAVVALPTSTGYGMADRGETALRAALASCSPGVVAVNIDNGYGAACAALRILN
                     ATGARVAAERPGRDAG"
     gene            1294..2601
                     /gene="larA"
                     /locus_tag="VGB88_13815"
     CDS             1294..2601
                     /gene="larA"
                     /locus_tag="VGB88_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX9648577.1"
                     /translation="MQVELRYGKGGLDITLDPAWRARLVRKPSMPAITDPPSAVRRAL
                     AHPVAAPALVEAAAGARSACILICDITRPVPNGTVLPVLVRALIGAGVPAEAITVLIA
                     TGLHRPNEGAELRELVGDDWVLETVRVENHDARDDAAHVHVGTTTRGNVVRLDRRFVD
                     AELKIVTGLVEPHFMAGYSGGRKVIAPGVAHRDTITTFHSARYMEDANAVNCVLDRNP
                     LHGDQLEIVAMIGGAVAVNTVIDEGRRLSFVNYGEIVNSHLDAVAFARRFTEVPVAER
                     FPTVVTSAAGFPLDKTYYQTIKSMVAPLDILEPGGTLIVASQMSEGMGSAEFCDAQRR
                     LIRLGPERFLDSLIAKRHADIDEWQTEMQLKPMRIGKVQLYSTGLSGSDSALTGVEMI
                     DSVEQAIRDSVARAGDSRVALVPEGPYVVPQYRPEAESRRSAA"
     gene            complement(2636..2971)
                     /locus_tag="VGB88_13820"
     CDS             complement(2636..2971)
                     /locus_tag="VGB88_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648578.1"
                     /translation="MPYVKRGPGGRVAALFAEAQADAREFVRADADEVRRFIGAGGAA
                     DDPRRSLTESDFELVRVLEDLISVLLDKNIIQQTDLPQEARAKLLKRRQLRGELDNLV
                     GLVAEEEDI"
     regulatory      3090..3292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3483..4046
                     /locus_tag="VGB88_13825"
     CDS             3483..4046
                     /locus_tag="VGB88_13825"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9648579.1"
                     /translation="MVKLDKIYTRGGDKGATSLGDGSRVPKHALRVDAYGTVDEANAA
                     IGLARLATDGEADAMLARIQNDLFDLGADLCLPAGSRKGKGALRIVDSQVARLEAEID
                     SLNAELAPLDSFILPGGSRASANLHFARTVVRRAERLVAELGEAETVAAPALAYLNRL
                     SDHLFVLARWLNDRGRGDVLWTPGANR"
     gene            4043..5986
                     /locus_tag="VGB88_13830"
     CDS             4043..5986
                     /locus_tag="VGB88_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9648580.1"
                     /translation="MTRAGWLAAGLVLAAAPAAATDAPAYLLPDVVVTPLRAPTPAAR
                     SASTVSVIEGDVIETRQQRDIVEVLRTVPGLNVVQTGPRGAQASVFTRGTNSNHTLVL
                     LNGIQIGDPSVPSNAFDFAHLTADRIERVEVLRGPQATIYGADAIGGVVNIITRRGDG
                     PPGGFVEVEGGSFSTHAERLGVSGGTALYDYDLGVARFETAGVSITPPRLWPGALAVD
                     ERDGYDNLTGQVQLGLRPLDGIELRAYGRRTNTRQDNDSFLNDPNAETKSFQTFARAE
                     ASAELFDGVLETRLGYALTVHDRKNTDDPDAVDPVEVSDFKDQGRRTVVDLQGTLNAI
                     PGNTLVFGAEYERESMSSSNFSIDQFGSLISGGTHEDDEVYAGYLQDTVAIGERLSVT
                     GGVRFDAHQSFGLQTTWRAGASYDLAETGSRLKASYATGFVAPSLFQRYGLSTFTSAF
                     FSSTFVGNPALKPERSRSWEAGIEQDLLDGMVTFGAAYFETEIDNLITSNATFTTNIN
                     LGRADIHGVESFLALRPVDWAELRLDHTYLRAEDADIGEDLLRRPKHKASAALTLYPV
                     DGASVTLDALYVGRRVDGDALLFARITKGSYTVVNLAGDYRLGEHVRVFARVENLFDR
                     DYEDPDGFAHPGLGAFVGVRLSL"
     gene            5983..6819
                     /locus_tag="VGB88_13835"
     CDS             5983..6819
                     /locus_tag="VGB88_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9648581.1"
                     /translation="MSWRAAALLLALLVAAGTAEARPERVVSVNLCTDILTLLVAAPG
                     QVASVTWLGADPDESPVADLAAGLAVNHGAAEEVMRLDADLVVAADYSAQASLALLRR
                     LGVPVVEVAVEDSVAGVAAALKTLGEALGVPENAAAVVARLNGGLARLAATAPGRGRT
                     ALVYRTGGYVAARPGLIDHMLAALGLDNLAAARGFAAGTQPSVEELLLARPELLVVES
                     YRPGAPSLAEARLGHRALAALRDSAQVVAVPAPLWACPSPFIADAAEAVADALIAGDE
                     PR"
     gene            6830..7816
                     /locus_tag="VGB88_13840"
     CDS             6830..7816
                     /locus_tag="VGB88_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9648582.1"
                     /translation="MTAPAPWGLYLAGAVLLVAAAAGSVLVGPGGGLFALWRDDPDLA
                     SLIAFEIRLPRLALALVTGATLGLSGAALQGLLRNPLAEPGLIGASSSAALGAVLVLY
                     FGLSAAVPMALPLAGMAGALVSVVLLAALVGGEASTLTLILAGIAINSFATALTALAL
                     NLAPNPYAVYEIVFWLLGSVADRDLDQLALIAGPVALGWLLLLGAGRGLDALSLGEEV
                     AQSLGVRTGRLFAQVVVGTALAVGAVVSVTGVIGFVGLVVPHLVRPLLGHQPGRLLPA
                     SALAGAILLVVADIAVRLAAPAGGELRLGVVTALVGAPFFLWLLLETRRRML"
     gene            7813..8598
                     /locus_tag="VGB88_13845"
     CDS             7813..8598
                     /locus_tag="VGB88_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648583.1"
                     /translation="MIEARGVSLSLAGREVLRAVDFAAAAGQTVGVVGPNGAGKTSLV
                     RVLAGLIAPAAGEVRLDGRPLGSWPRPARARRLAYLPQGETVHWPITAGRLVALGRLP
                     HLVAWQRPRPEDAAAIERAMAAADVVALAGRRMPELSAGERRRVLIARALAVEAPVLL
                     ADEPVAGLDPYHQLEILELLRATAAAGRTVVVVLHDLALAARFCDRVAVLRDGAVLAA
                     GPARATLGAETIARAFDVDVATVVANGEAAPLPWRRRPKAAGS"
     gene            8624..>9347
                     /locus_tag="VGB88_13850"
     CDS             8624..>9347
                     /locus_tag="VGB88_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9648584.1"
                     /translation="MARPVAYDNAPIAPGIRSRFVERINGLTVHLLEAGFEAKGRPCV
                     VLLHGFPELAYSWRKVMPAIAAAGFHVIAPDQRGYGLTTGWDGSYDGDLASFRPLNLV
                     RDVLALVLALGHRSVAAVVGHDFGARVAAGCALVRPDIFRAMVLMSAPFPGPPPPAST
                     VPAGPSIHDQLAALDPPRKHYQWYYSTRHANDDMRHCAQGVHAFLRAYFHVKSADWKP
                     NRPYRLASWSAGELAKLPTYYVM"
BASE COUNT         1174 a         3455 c         3372 g         1346 t
ORIGIN      
        1 ggctgggcgc tgtcggtggt cgaggccggc gagttcgccg acccggccta cctcgccaac
       61 ccgcacgacc gttgcttcca ctgcaagacc aacctttacc gcacgatcgg cgcgctgacc
      121 gcgcgcacgg tggtttcggg cgccaacctc gacgatctcg ccgactatcg ccctggcctc
      181 gccgccgccg cgcggcacgg cgtgcgccat ccctatgtcg aggcgaacat cgacaaggca
      241 tgcgtgcgcg cgctcgccgc cggtcttggc ctcgacgagg tcgccgacct gccggccgcg
      301 ccgtgcctgt cgagccgggt cgagaccggc atcgccatcg acgccgacat gctgcgcctg
      361 atcgacgccg ccgagcgctt cgtccgccgg cgcgtgcgcc cggacacggt gcgctgccgg
      421 ctgcgcggct cgggcgtcgt gatcgagctc gacgagcctg cgctcgcccg ccttgaggga
      481 ggcgccgcgg catccctcgc cgaggcggtg gcggcgatgt tcgcccgcgc cggcgcgcgc
      541 cgcgcggtgc gcgtcgcgcc ctacgtccgc ggcagcgcgt tcgtcggcgg ggcgcggccg
      601 tgaacgacgt cgcgcgcgag gtcaggctcg acttcggccg cgggccccgg atcggcctgg
      661 acgaggcgat cctatgtgag cacaagagct tagaacagat cgttcatatt atggagcagg
      721 cgcgggtgcg cgcgcgcagc ctgctgctga cgcgcctcgg cgctgacctg gcggcggccc
      781 tgcccgagca gctcgcggcc gcgctcgact actgcgcgac ctcgcgcacg gcgttcttcg
      841 gctcggtcgc ggcgccgagc gcgccggcgc gcgtcgccgt ggtcgcgggc ggcagctcgg
      901 acgccccggt cgcgcgcgag gcggcgcgca ccctcgcctt tgccggccag gcgagcgagc
      961 tcgccttcga cgtcggcgtc gccgggctgt ggcggctgct cgacgagatc gggcggctcg
     1021 agcgcatggc ggtagtgatc gtcgccgcgg gcatggacgc ggcgctggtc agcgtcgtcg
     1081 ccggcctggt gccgggtgcg gtggtggcat tgccgacctc gaccggctac ggcatggccg
     1141 accgcggcga gaccgcgctc agggcggcgc tggcgagctg cagcccgggc gtggtcgcgg
     1201 tcaacatcga caatggttac ggcgccgcct gcgccgcgct gcgcatcctc aacgcgaccg
     1261 gcgcgcgcgt tgcagccgaa aggccgggcc gcgatgcagg ttgagctccg ctacggcaag
     1321 ggcgggctcg acatcacgct cgacccggcg tggcgggcgc gcctggtgcg caagccgtcg
     1381 atgccggcga tcacggatcc cccttcagcg gtgcgccgcg cgctggccca cccggtggcg
     1441 gcgccggcgc tggtcgaggc cgcggccggg gcgcgctcgg cctgcatcct gatctgcgac
     1501 atcacccggc cggtgccgaa cggcaccgtg ctgccggtgc tggtgcgcgc gctgatcggc
     1561 gccggggtgc cggcggaggc gatcaccgtg ctgatcgcga ccggcctgca ccggcccaac
     1621 gagggcgcgg agctgcgcga gctggtcggc gacgactggg tattggagac cgtacgggtg
     1681 gagaaccacg acgcgcgcga cgacgccgcc cacgtccatg tcggcaccac cacccgcggc
     1741 aacgtcgtcc gcctcgaccg gcgcttcgtc gacgccgagc tcaagatcgt caccggcctg
     1801 gtcgagccgc acttcatggc cggctactcg ggcgggcgca aggtgatcgc ccccggcgtc
     1861 gcccaccgcg acaccatcac caccttccac agcgcccgct acatggagga tgccaacgcc
     1921 gtcaactgcg tgctcgaccg caacccgctg cacggcgacc agcttgagat cgtggccatg
     1981 atcggcggcg cggtcgcggt caacacggtg atcgacgagg gccgccggct gtcgttcgtc
     2041 aactacggcg agatcgtcaa cagccacctc gacgcggtcg ccttcgcccg ccgcttcacc
     2101 gaggtgccgg tggccgagcg cttcccgacc gtggtcacca gcgccgccgg ctttccgctc
     2161 gacaagacct actaccagac catcaagagc atggtcgcgc cgctcgacat cctcgagccc
     2221 ggcggcacct tgatcgtcgc ctcgcagatg tccgaaggca tgggctcggc cgagttctgc
     2281 gacgcccagc gccgcctgat caggctcggg cccgagcgct tcctcgacag cctgatcgcc
     2341 aagcgccacg ccgacatcga cgaatggcag accgagatgc agctcaagcc gatgcgcatc
     2401 ggcaaggtcc agctctacag caccgggctc agcggtagcg acagcgcgct caccggggtg
     2461 gagatgatcg actcggtcga gcaggcgatc cgcgactcgg tcgcgcgcgc cggggattcg
     2521 cgggtggcgt tggtgcccga agggccgtac gtcgtgcccc aataccgccc cgaggccgaa
     2581 agccggcgca gcgccgcgtg agcgccgagg cggcgcggcg caacgtgcgc cggcctcaga
     2641 tgtcctcttc ctcggccacc aggccgacca ggttgtcgag ctcgccgcgc agctggcgcc
     2701 gcttgagcag cttggcgcgc gcctcctggg gcaggtcggt ctgctggatg atgttcttgt
     2761 ccagcagcac gctgatcagg tcctcgagca cgcgcaccag ctcgaagtca gactcggtca
     2821 ggctgcggcg cgggtcgtcg gcggcgccgc cggcaccgat gaagcggcgc acctcgtcgg
     2881 cgtcggcgcg cacgaactcg cgcgcgtccg cctgcgcctc ggcgaacagg gctgccacgc
     2941 gcccgcccgg tcctcgcttg acgtacggca tgggtcgcct cctcggggcg tcccgcttcg
     3001 gtaatcgatt cgccccgata aagtgtagac cgcgcccggg cccgggcaaa tccggacgct
     3061 cggtcgggat tgacctttcc cggccgcgct ctatcatcgg cgccggtggt tccccgttgg
     3121 gggattgaaa gggaacgcgg tgcggccggc actcgtccgg ccaataccgc ggctgccccc
     3181 gcaactgtgg gcgggtggcc cgtcgccgca acacgccact gggcaactgg gaaggcgggc
     3241 ggcgggctca gatccgcgag ccaggagacc tgccaccatc gtcagtcaac gtgaaggcgc
     3301 cggacggggt gtgccggcga cggtcatgtc gctcgaaacc agttacccac agtcccaggt
     3361 tttcggccgg cacgcgcccg cattcgcgct gacgcagcac gtcgcgggcg gtggcggcac
     3421 cgcgcgcgac cggatgtgat ccgcccgcgc cggtgcgcgg tcggacgcgg agggacgcac
     3481 cgatggtcaa gctcgacaag atctataccc gcggcggcga taagggggcg acgtcgctcg
     3541 gcgacggcag ccgggtgccg aagcacgcgc tacgggtgga cgcctacggc accgtggacg
     3601 aagccaatgc cgcgatcggg ctcgcgcgcc tggccacgga cggcgaggcc gacgcgatgc
     3661 tcgcgcgcat ccagaacgac ctgttcgacc tcggcgccga cctgtgcctg ccggcaggct
     3721 cgcgcaaggg caagggcgcg ctcaggatcg tcgactccca ggtcgcgcgg ctcgaggccg
     3781 agatcgactc gctcaatgcc gagctggcgc cgctcgactc cttcatcctg cccggcggca
     3841 gccgggcctc ggcgaatctg catttcgcgc gcacggtggt ccgccgggcg gagcggctgg
     3901 tggccgagct cggcgaagcc gagacggtcg ccgcgcccgc gctcgcctat cttaaccggc
     3961 tgtccgatca cctgttcgtg ctcgcccgct ggctcaacga ccgcggccgc ggcgacgtgc
     4021 tgtggacgcc gggggccaac cgatgacgcg cgccggctgg ctcgccgcag gcctcgtgct
     4081 cgcggcggcg ccggcggcgg caaccgatgc tcccgcctac ctgctgcccg acgtcgtcgt
     4141 cacgccgttg cgcgcgccca cgccggcggc gcgatccgcg agcacggtga gcgtgatcga
     4201 gggcgacgtc atcgagaccc gccagcagcg cgacatcgtc gaggtgctgc gcaccgttcc
     4261 cgggctcaac gtggtccaga ccgggccgcg cggcgcgcag gcctcggtgt tcacccgcgg
     4321 caccaactcg aaccacaccc tggtcctgct caacggcatc cagatcggcg atcccagcgt
     4381 gcccagcaac gccttcgact tcgcccatct gactgccgac aggatcgagc gcgtggaggt
     4441 gctgcgcggc ccgcaggcga ccatctatgg cgcggatgcg atcggcggcg tggtcaacat
     4501 catcacccgc cgcggcgacg gcccgccggg cggcttcgtc gaggtcgagg gcggcagctt
     4561 ctcgacccat gccgagcgcc tcggcgtcag cggcggcacc gcgctgtacg actacgacct
     4621 cggcgttgcg cgcttcgaga ccgcgggcgt ctcgatcacg cccccgcgcc tgtggccggg
     4681 ggcgctcgcc gtcgatgagc gcgacggcta cgacaacctg accgggcagg tccagctcgg
     4741 actgcgcccg ctcgatggga tcgagctccg cgcctatggg cggcgcacca atacacgcca
     4801 ggacaatgat tcgttcctga acgatcccaa tgccgagacc aagagcttcc agaccttcgc
     4861 ccgcgccgag gcgtcggccg agctgttcga cggcgtgctc gagacccggc tcggctacgc
     4921 gctcaccgtg cacgaccgca agaacaccga cgaccccgat gcggtcgatc cggtcgaggt
     4981 atcggacttc aaggaccagg gccggcgcac cgtggtcgac ctccagggca ccctcaacgc
     5041 gatccccggc aacaccctgg tgttcggcgc cgagtacgag cgcgagagca tgtcgagctc
     5101 gaacttcagc atcgaccagt tcggcagcct gatcagcggc ggcacccacg aggacgacga
     5161 ggtctatgcc ggctacctcc aggacaccgt cgccatcggc gagcggctgt cggtgaccgg
     5221 cggcgtgcgc ttcgacgccc atcagagctt cggcctgcag accacctggc gggcgggggc
     5281 gagctacgac ctcgcggaga cgggcagccg cctcaaggcg agctacgcca ccggcttcgt
     5341 cgcgccgagc ctgttccagc gctacggctt gtccaccttc acctcggcct tcttcagctc
     5401 gaccttcgtc ggcaacccgg cgctcaagcc cgagcgcagc cgaagctggg aggccggcat
     5461 cgagcaggac ctgctcgacg gcatggtgac cttcggcgcg gcctatttcg agaccgagat
     5521 cgacaacctg atcacctcca acgccacctt caccaccaac atcaacctcg gccgggcgga
     5581 catccacggc gtcgagtcgt ttctcgccct gcgcccggtc gactgggcgg agctgaggct
     5641 cgaccacacc tacctgcgcg ccgaggacgc cgacatcggc gaggacctgc tgcgccggcc
     5701 gaagcacaag gcgagcgcgg cgctgaccct gtacccggtc gacggcgcca gcgtcaccct
     5761 cgatgcgctc tatgtcggcc gccgcgtgga cggcgacgca ttgctgttcg cgcgcatcac
     5821 caagggcagc tacacggtgg tcaacctggc cggcgactac cggctcgggg agcatgtgcg
     5881 cgtgttcgcg cgcgtcgaga acctgttcga ccgcgactac gaggatccgg acggcttcgc
     5941 ccatcccggg ctcggcgcgt tcgtcggcgt gcggctgtcg ctgtgagctg gcgtgcggcg
     6001 gccctgctgc tggcactgct ggtcgcggcc ggcaccgccg aggcgcggcc cgagcgggtg
     6061 gtgtcggtca acctgtgcac cgacatcctg accttgctgg tcgccgcgcc cggccaggtc
     6121 gcctcggtga cctggctcgg cgccgatccg gacgaatccc cggtcgccga cctcgccgcc
     6181 gggctggcgg tcaaccacgg cgcggccgag gaggtgatgc gcctcgacgc cgacctggtg
     6241 gtcgcggctg actactcggc gcaggcgagc ctcgccctgc tgcggcggct cggtgtcccg
     6301 gtcgtcgagg tcgcggtcga ggactcggtt gccggcgtcg cggccgcgct gaagacgctg
     6361 ggcgaggcgc tcggggtgcc ggaaaacgcg gcggcggtgg tcgcgcgcct gaacgggggt
     6421 ctcgcgcggc tcgcggcaac ggcgccgggg cggggacgga cggcgctggt ctatcgcacc
     6481 ggcggctacg tggcggcgcg accggggttg atcgatcaca tgctggcggc gctcggcctc
     6541 gacaacctgg ccgcggcgcg cggctttgcc gcgggcacgc agccatcggt cgaggagctg
     6601 cttctcgccc gccccgagct gcttgtggtc gagtcctatc gcccgggcgc accctcgctg
     6661 gccgaggcgc ggctcggcca tcgggcgctc gccgcgttgc gcgactcggc ccaggtggtc
     6721 gccgtgccgg cgccgctgtg ggcgtgcccg agcccgttca tcgccgacgc cgccgaggct
     6781 gtcgccgatg ccttgatcgc gggcgatgag ccgaggtgac gtgcgcgcga tgaccgcgcc
     6841 cgcgccgtgg gggctctacc tcgcgggcgc ggtgctgctg gtcgcggcgg cggcgggctc
     6901 ggtgctggtc ggcccgggcg gaggcctgtt cgccctgtgg cgcgacgacc ccgacctcgc
     6961 ctcgctgatc gcgttcgaga tccgcctgcc gcggcttgcg ctggcgctgg tcaccggcgc
     7021 gaccctcggc ctcagcggcg ccgcgctcca ggggctgctg cgcaacccgc tcgccgagcc
     7081 cggcctgatc ggcgcctcga gcagcgcggc cctgggtgcg gtgctggtgc tctatttcgg
     7141 gctgagcgcg gcggtgccga tggcgctgcc gctcgccggc atggccgggg cgctggtctc
     7201 ggtggtgctg ctcgcggctc tggtcggggg cgaggcgagc acgctcaccc tgatcctcgc
     7261 cggcatcgcc atcaacagct tcgccaccgc gctcaccgcg cttgcgctca acctcgcgcc
     7321 caacccctac gcggtctacg agatcgtgtt ctggctgctc ggctcggtcg ccgaccgcga
     7381 cctcgaccag ctggcgctga tcgccggccc ggtggcgctc ggctggctgc tgctgctcgg
     7441 cgccgggcgc gggctcgatg cgctgagcct gggcgaggag gtggcgcaga gcctcggcgt
     7501 gcgcaccggg cggctgttcg cgcaggtcgt cgtcggcacc gcgctcgcgg tcggcgcggt
     7561 ggtctcggtg accggcgtga tcggtttcgt cggcctggtg gtgccgcacc tggtccgccc
     7621 gctgctcggc caccagccgg ggcggctgct gccggcgagt gcgctcgccg gcgcgatcct
     7681 gctggtggtc gccgacatcg cggtgcggct cgccgcgccg gccggcggcg agctccgcct
     7741 cggcgtggtc accgcgctgg tgggtgcgcc gttcttcctg tggctgctgc tcgagacgcg
     7801 caggcgaatg ctgtgatcga ggcgcgcggc gtctcgctca gcctggccgg gcgcgaggtc
     7861 ctgcgcgcgg tcgacttcgc cgccgcggcc gggcagacgg tgggcgtggt cgggccgaac
     7921 ggcgccggca agacctcgct ggtgcgggtg ctcgccggcc tgatcgcgcc ggccgccggc
     7981 gaggtcaggc tcgacggccg tccgcttggg tcgtggccgc ggccggcgcg cgcgcgcagg
     8041 ctcgcttatc tgccccaggg cgagaccgtg cattggccga tcaccgccgg acggctggtg
     8101 gcgctcggcc ggctgccgca cctggtggcg tggcagcgcc cgcgccccga ggacgcggcg
     8161 gcgatcgagc gcgccatggc cgcggccgat gtcgttgccc tggcagggcg gcggatgccc
     8221 gagctgtcgg cgggcgagcg gcgccgggtg ctgatcgcgc gcgcgctcgc ggtcgaggcg
     8281 ccggtcctgc tcgccgacga gccggtcgcc ggcctcgatc cctaccacca gctcgagatc
     8341 cttgagctgt tgcgcgccac cgccgcggcc gggcggacgg tggtggtggt gcttcacgac
     8401 ctcgcgctgg cggcgcgctt ctgcgaccgg gtggcggtgc tgcgcgacgg cgcggtgctc
     8461 gccgccgggc ccgcgcgcgc gaccctcggg gccgagacca tcgcgcgcgc gttcgatgtc
     8521 gatgtcgcca ccgtggtcgc gaacggcgag gccgcgccgc tgccctggcg ccgccgcccc
     8581 aaggccgcgg gttcctgata ggctcgtgcc gatggagacc cgcatggccc ggcccgtcgc
     8641 ctacgacaac gcccccatcg cgcccggcat ccgctcgcgc ttcgtcgagc ggatcaacgg
     8701 cctgacggtt cacctgctcg aagcgggctt cgaggccaag ggccggccat gcgtcgtgct
     8761 gctgcacggc tttccggaac tcgcctacag ctggcgcaag gtgatgcccg ccatcgccgc
     8821 ggcggggttt cacgtcatcg cgcccgacca gaggggctac gggctgacca ccggctggga
     8881 cggcagctac gacggcgacc tcgcctcgtt ccgcccgctc aacctggtgc gcgacgtgct
     8941 cgcgctcgtg ctcgcgctcg gccaccgctc ggtcgcggcc gtggtcgggc acgatttcgg
     9001 cgcgcgcgtc gccgccggct gcgcgctcgt ccgcccagat atttttcgcg cgatggtgct
     9061 gatgagcgcg ccgttcccgg gcccgccgcc gccggcgagt accgtccccg ccggccccag
     9121 catccacgac cagctcgccg cgctcgaccc gccgcgcaag cattaccagt ggtactactc
     9181 gacgcgccac gccaacgacg acatgcgcca ctgcgcccaa ggcgtgcacg cgttcctgcg
     9241 cgcctacttc cacgtcaaga gcgcggactg gaaaccgaac cgcccgtacc ggctcgcgtc
     9301 gtggagcgcc ggcgagctcg ccaagctgcc gacctattac gtgatgg
//
LOCUS       DASWHU010000372         7813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2854000, whole genome shotgun sequence.
ACCESSION   DASWHU010000372 DASWHU010000000
VERSION     DASWHU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854000"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGB88_13855"
     CDS             complement(<1..491)
                     /locus_tag="VGB88_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648585.1"
                     /translation="MAINADLSLRAHADTAAMAWAASPAKGVARKRVYHDGAPESGRV
                     TSIVRYAAGSRFPAHEHPEGEEIVVLAGTFSDHTGDWGPGSYLLSPEGSPHAPWSEHG
                     CQLFVHLRQYRGAQRLRIDTAARAWRRTSIPGIAHKVLLNEHDGPDGPLMVRLMRFRP
                     GSER"
     gene            complement(659..2098)
                     /gene="tldD"
                     /locus_tag="VGB88_13860"
     CDS             complement(659..2098)
                     /gene="tldD"
                     /locus_tag="VGB88_13860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX9648586.1"
                     /translation="MTDIAKLEDLFFSRTGMDAGRVADIVADALGGADDGELFLEYRQ
                     SESLAFDDGRLKSASFDTSQGFGLRAVSGEATGYGHASELSEAAIRRAAETVKAVRTG
                     RGGQADVGPHGSNLELYREDNPLNAVDFAAKVALLERMDAYARARDSRVRQVMAALSG
                     SWQAVEIVRADGARVGDIRPLVRLNVSVVVGDGAHMETGSHGSGGRYGYDILLDETTW
                     REHVDEALRQALVLLDARDAPAGEMQVVLGPGWPGILLHEAIGHGLEGDFNRKRTSVF
                     SGLMGEKVAAGGVTVIDDGTLESRRGSLTVDDEGTPTQRTTLIEDGTLVGYMHDRLNA
                     RLMGARSTGNGRRQSFAHQPMPRMRNTIMVAGEADPADIIASVGKGLYAVNFGGGQVD
                     ITSGKFVFSASEAYLIEAGKVTAPVKGATLIGTGHEVLKRVRAIGNDMRLDPGIGTCG
                     KNGQGVPVGVGLPTLLVDKLTVGGTEALG"
     gene            2218..2670
                     /locus_tag="VGB88_13865"
     CDS             2218..2670
                     /locus_tag="VGB88_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648587.1"
                     /translation="MTATAPRVTLAGDGFALSAPAPRAGERIEAPGRGPRALLAAPCL
                     EHRPSAAPAAFARAAAARRPDLSLIALSADDDAVRARWCRHAGWRPGRCPPEAAARLG
                     IWLPRLQLFAPALLVIDAAGRLCHAEIVRDLAAEVDFDAALAALAGLA"
     gene            2667..3995
                     /locus_tag="VGB88_13870"
     CDS             2667..3995
                     /locus_tag="VGB88_13870"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEX9648588.1"
                     /translation="MSDDRRPLLGGLVEHPERAALAAEVHARPPMRLGAPAKLSHLVM
                     LSGEAAAADERAHLGLLADSLDVAPPHHAARQFFADFGAFKLRWERHTEFAAWTFVRE
                     GAAAAATPALAEAFADLPIGHVPEDWLSALPGQVLAHIDLAMLARATSPPTPQQIEEL
                     FDHPVGGLVGDGAARAWTDFRLYGGGMRILVHDLKLTSGRRGRLVQRLLEIETYRMLA
                     LLAFPLVRSFGPQMTEIDARLVDLTARLRDAGESATPRALLDEALALGAEVERISGAT
                     SYRLSAARAYHELVERRIDELAERRSAPGETGLQPIGQFLRRRLTPAMRTCESLRERQ
                     ETAARHLERASHLLRSRIELDLAEQNRAQLAAMNRRSRLQLRLQQTVEGLSVAAITYY
                     VVGLVAIVFRSLPESWLVLSPERASGIAVPAVLVLAWLLVRRIRSRRRSG"
     gene            4194..5045
                     /gene="coxB"
                     /locus_tag="VGB88_13875"
     CDS             4194..5045
                     /gene="coxB"
                     /locus_tag="VGB88_13875"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9648589.1"
                     /translation="MASNSFVMRTVAAAAAVVLPVGVALAEPVAGRPVPWQMGFQPPA
                     SPVMEQIESFHGLLLVIITLIASLVLGLLVYVMIRFNAKRNPTPSRITHNTVIEVLWT
                     VVPVLVLVVIAIPSFRLLYYELELPDADITIKTIGHQWYWSYEYPDEGFTFDAFMVED
                     DSLEEGQPRLLTADNPLVVPVDAVVRLQVTADDVLHSWALPAFGVKVDAVAGRLNEIW
                     FEANQTGTFYGQCSELCGTLHAFMPIEVHVLSKDDYAAWLVQARAEFARIDGEDARVR
                     LARTGAR"
     gene            5081..6694
                     /gene="ctaD"
                     /locus_tag="VGB88_13880"
     CDS             5081..6694
                     /gene="ctaD"
                     /locus_tag="VGB88_13880"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX9648590.1"
                     /translation="MSNLDAAAGHPHHPTGVLRWLYSTNHKDIGTMYLVFAAVGGLIG
                     GTFSMLIRSELSLPGVGVLGDSITDASHVYNVIITAHGLIMIFFMVMPAMIGGFGNWF
                     VPIMIGAPDMAFPRMNNISFWLLIPSFILLLGSAFVDGGPGNGAGTGWTIYPPLSSAG
                     SHAGPAVDMAIFALHLAGASSILGAINFITTIFNMRAPGMTLHRMPLFVWSILVTAFL
                     LLLTLPVLAGAITMLLTDRNFGTAFFKSELGGDPVLFQHLFWFFGHPEVYILILPGFG
                     IISQIVATFSRKPIFGYLGMAYAMVAIGVVGFVVWAHHMYTVGLAVDTRAYFTAATLI
                     IAVPTGIKIFSWIATMWGGSIEFRTPMLYAIGFIFLFTIGGVTGVVLANAGINIALHD
                     TYYVVAHFHYVLSLGAVFAVFAGFYYWIGKMSGRQYPETLGKIQFWTMFIGVNLTFFP
                     MHFLGLAGMPRRYIDYPDAFAPWNMVASIGSYVSGASVLLFMYIVWRTFTTGARCAAN
                     PWGEGATTLEWRTSSPPPFHTYEELPQVR"
     gene            6723..7646
                     /locus_tag="VGB88_13885"
     CDS             6723..7646
                     /locus_tag="VGB88_13885"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9648591.1"
                     /translation="MSEMSSAIGEAPAAGAGPADFVALLKPRVMSLVCFTGLAGMLLA
                     PGSLHPVIAFTALLCMAVGAGAAAAINMWCDRDIDALMARTRARPIPAGRVAAADALG
                     FGVTLAVGAVATMGLAVNWTAAALLALTIAFYVFVYTMWLKRRTPQNIVIGGAAGALP
                     PVIGWAAMSGSVTLEPMLLFLIIFLWTPPHFWALAILKAEEYGRAGVPMMPNIAGPAS
                     TRRQIFVYALAVAACALLPAALGFAGIAYGVAAALLGLEFLRRAWRLLRADRDLAPAR
                     RLFGYSIFYLFAIFAALLIERLLVPATGVAS"
     gene            7643..7798
                     /locus_tag="VGB88_13890"
     CDS             7643..7798
                     /locus_tag="VGB88_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648592.1"
                     /translation="MKPERVELTEREKKARSARNIAIALALAAFVILLYVATWAKLGA
                     NILNRPL"
BASE COUNT         1016 a         2799 c         2701 g         1297 t
ORIGIN      
        1 cgctcgctcc ccggcctgaa cctcatcagc cgcaccatca gcgggccgtc cggcccatca
       61 tgctcgttga ggagcacctt gtgggcgatg cccggaatgc tggtgcgccg ccaggcgcgc
      121 gccgcggtgt cgatgcgcag cctctgcgcg ccgcgatact ggcgcaggtg cacgaacagc
      181 tggcagccgt gctccgacca cggcgcgtgc ggcgagcctt ccgggcttag caggtagctt
      241 cccgggcccc agtcgccggt gtggtcggag aaggtgccgg cgagcaccac gatctcctcg
      301 ccctcagggt gctcgtgcgc cgggaagcgc gagcccgccg catagcgcac gatcgaggtc
      361 acccgcccgg actcaggcgc gccgtcgtga tagacgcgct tgcgcgccac ccccttggcc
      421 gggctcgcgg cccacgccat ggcggcggtg tcggcgtgcg cgcgcaagga caggtcggcg
      481 ttgatcgcca ccgttcccgc cagctcagaa gcccggcgcc ggtcgcctca cccttcgaga
      541 cgcgccccgc gggcgctcct cagggtgagg taattcattg acattcaaga tgtcctcgtc
      601 ctgaggagcg agcgcagcga gcgtctcgaa ggacaacggg cgggtcattc gcccaccctc
      661 agccgagcgc ctcggtgccg cccacggtca gcttgtcgac caagagggtc ggcaggccga
      721 cgccgaccgg caccccctgg ccgttcttgc cgcaggtgcc gatgccgggg tcgaggcgca
      781 tgtcgttgcc gatcgcgcgc acgcgcttga gcacctcgtg gccggtgccg atcagggtcg
      841 cgcccttgac cggggcggtg accttgccgg cctcgatcag gtacgcctcg ctggccgaga
      901 acacgaactt gccgctggtg atgtcgacct ggccgccgcc gaagttgacc gcgtagagcc
      961 ccttgccgac cgaggcgata atgtccgcgg ggtcggcctc gcccgcgacc atgatggtgt
     1021 tgcgcatgcg cggcatcggc tggtgggcga agctctggcg gcggccgttg ccggtcgagc
     1081 gcgcgcccat caggcgggcg ttcagccggt cgtgcatgta gccgaccagg gtgccgtcct
     1141 cgatcagggt ggtgcgctgg gtcggcgtgc cctcgtcgtc gacggtgagc gagccgcggc
     1201 gcgattccag cgtgccgtcg tcgatcacgg tcaccccgcc cgccgcgacc ttctcgccca
     1261 tgagccctga gaacacgctg gtgcgcttgc ggttgaagtc gccctcgagg ccgtggccga
     1321 tcgcctcgtg caacaggatt cccggccagc cggggccgag caccacctgc atctcgcccg
     1381 ccggcgcatc gcgcgcgtcc agcagcacca gcgcctggcg cagcgcctcg tcgacatgct
     1441 cgcgccaggt cgtctcgtcg agcaggatgt cgtagccgta gcgcccgccc gagccgtggc
     1501 tgccggtctc catgtgggcg ccgtcgccga ccaccaccga gacgttgagg cgcaccagcg
     1561 ggcggatgtc gccgacccgg gcgccgtcgg cgcgcacgat ctcgaccgcc tgccagctgc
     1621 ccgacagcgc cgccatcact tggcggacgc gcgagtcccg cgcgcgggcg taggcatcca
     1681 tccgttccag cagcgcgacc ttggcggcga agtcgaccgc gttcagcggg ttgtcctcgc
     1741 ggtacagttc gaggttgctg ccgtgcgggc cgacatcggc ttggccgccg cggccggtgc
     1801 gcaccgcctt gaccgtctcg gcggcgcgcc ggatcgccgc ctcgctcagc tccgaggcgt
     1861 ggccgtagcc ggtcgcctcg cccgagaccg cgcgcaggcc gaatccctgc gaggtgtcga
     1921 agctcgccga cttgagccgg ccgtcgtcga aggcgaggct ctcggactgg cggtactcca
     1981 ggaacaactc gccgtcgtcg gcgccgccga gggcatcggc gacgatgtcc gcgacccgtc
     2041 cggcgtccat gccggtgcgg ctgaagaaca ggtcctcgag cttggcgatg tcggtcatgg
     2101 tctccgctcc ggccgggatt ttgtcgcgcg cgcgctccgt acgtcgccta tatgtagcgg
     2161 ggccgggccg ccaatccaac cggcggccgg cgaacaaggt gacggatggc cccgggcatg
     2221 accgcgaccg cgccaagggt gacgctcgcc ggcgacggct tcgcgctgtc ggcgccggcg
     2281 ccgcgggcgg gtgagcgaat cgaggccccc ggccgcgggc cgcgcgcgct gctcgccgct
     2341 ccctgcctcg agcaccgccc gagcgcggcg ccggcggcgt tcgcgcgcgc cgcggcggcg
     2401 cggcgccccg acctgtccct gatcgcgctc agcgccgacg acgacgcggt gcgcgcgcgc
     2461 tggtgccggc atgccggctg gcggccgggc cgctgcccgc ccgaggccgc cgcccggctc
     2521 ggcatctggc tgccgcggct gcagctgttc gcgccggcgc tgttggtgat cgacgcggcc
     2581 ggccgcctgt gccacgccga gatcgtgcgc gacctcgccg ccgaggtcga tttcgacgcc
     2641 gcgctggcgg cgctcgcggg gctggcatga gcgacgaccg ccggccgctg ctcggcgggc
     2701 tggtcgagca tcccgagcgc gccgcactcg ccgccgaggt ccacgcccgc ccgccgatgc
     2761 gcctcggcgc gccggccaag ctcagccatc tggtgatgct gtcgggcgag gcggcggcgg
     2821 ccgacgagcg cgcccatctc ggcctgctcg ccgacagcct cgatgtcgcc ccgccgcacc
     2881 acgccgcgcg ccagttcttc gccgacttcg gcgccttcaa gctgcgctgg gagcgccata
     2941 ccgagttcgc cgcctggacc ttcgtgcgcg aaggcgcggc ggcggcggcg acccccgcct
     3001 tggcggaggc gttcgccgac ctgccgatcg gccatgtgcc cgaagactgg ctgtcggcgc
     3061 tgcccgggca ggtgctcgcc cacatcgacc tcgccatgct cgcgcgcgcc acctcgccgc
     3121 ccacgcccca gcagatcgag gagctgttcg accacccggt cggcggcctg gtcggcgacg
     3181 gcgcggcgcg cgcctggacc gacttccggc tctacggcgg cggcatgcgc atcctggtcc
     3241 acgacctcaa gctgaccagc ggccggcgcg gccggctggt ccagcgcctg ctcgagatcg
     3301 agacctaccg catgctggcg ctgctcgcct tcccgctggt gcgcagcttt ggtccgcaga
     3361 tgaccgagat cgacgcccgg ctggtcgacc tgaccgcgcg cttgcgcgat gcgggcgaaa
     3421 gcgccacccc gcgcgcgctg ctcgacgagg cgctcgcgct cggcgccgag gtcgagcgta
     3481 tctcgggcgc gaccagctat cgcctcagtg cagcgcgggc ttatcacgag ctggtcgagc
     3541 ggcgcatcga cgagctcgcc gagcgccgca gcgcccccgg cgagaccggc ctgcagccga
     3601 tcggccagtt cctgcgccgg cggctgaccc cggcgatgcg cacctgcgag tcgctgcgcg
     3661 agcgccagga gaccgcggcg cggcatctgg agcgcgccag ccacctgctg cgcagccgca
     3721 tcgagctcga cctggccgag cagaaccgcg cgcagctcgc ggcgatgaac cggcgcagcc
     3781 ggcttcagct caggctccag cagacggtcg aagggctgtc ggtggcggcg atcacctatt
     3841 acgtggtcgg gctggtggcg atcgtgttcc gctcgctgcc cgagtcgtgg ctggtgctgt
     3901 cgccggagcg cgccagcggt atcgcggtgc cggcggtgct ggtgctggcg tggctgctgg
     3961 tgcgtcggat tcgcagccgt cgcagaagcg gttagcgcag ccgcgcgaac gcccgccggc
     4021 acgctcacga aggcgtgatc ggagacttga tttccgcgga cctgcgcggt tatatgcgcc
     4081 cgccccgcgc tgcgcgcccc aacggcgggg aacagcgtac atttcgcccc gcgccgggcg
     4141 agcggaaggc cctgccgctc cgcatcgagc cgaagtttga gggacgcttt ctcatggcat
     4201 cgaacagctt cgtcatgcgg acggtcgcgg cggcggcggc cgtggtgctg ccggtcgggg
     4261 tcgccttggc cgagccggtg gcgggccggc cggtgccgtg gcagatgggc ttccagccgc
     4321 cggcgagccc ggtgatggag cagatcgagt cgtttcacgg cctgctgctg gtgatcatca
     4381 cgctgatcgc cagcctggtg ctcgggctgc tggtttacgt gatgatccgg ttcaacgcca
     4441 agcgcaaccc gaccccgtcg cgcatcaccc acaacaccgt gatcgaggtg ctgtggacgg
     4501 tggtcccggt gctggtcctg gtggtgatcg cgatcccgag cttccgcctg ctctactacg
     4561 agctcgaatt gccggacgcg gacatcacga tcaagacgat cggccatcag tggtactgga
     4621 gctacgagta tccggacgag ggcttcacct tcgacgcctt catggtcgaa gacgacagcc
     4681 tcgaggaggg ccagcccagg ctgctgaccg ccgacaaccc gctggtggtg ccggtcgacg
     4741 ccgtggtccg gctccaggtc accgccgacg acgtgctgca ctcctgggcg ctgccggcgt
     4801 tcggggtcaa ggtcgatgcg gtcgccggcc ggctgaacga gatctggttc gaggccaacc
     4861 agaccggcac cttctacggc cagtgctcgg agctgtgcgg cacgctgcac gcgttcatgc
     4921 cgatcgaggt ccacgtgctg agcaaggacg actacgccgc ctggctggtg caggcgcgcg
     4981 cggagttcgc gcgcatcgac ggcgaggatg cgcgcgtgcg cctggcccga accggcgcgc
     5041 gctgacccgg cgggcgaaca cccagcggga agatggactg atgagcaatc tcgacgccgc
     5101 ggcaggacac ccgcatcacc cgaccggcgt gctgcgctgg ctgtactcga ccaaccacaa
     5161 ggacatcggc acgatgtacc tggtgttcgc cgcggtcggc gggctgatcg gcggcacctt
     5221 ttccatgctg atccgctcgg aactcagcct gcccggcgtc ggcgtgctcg gcgacagcat
     5281 cacggatgcg agccacgtct ataacgtgat catcaccgcc cacggcctga tcatgatctt
     5341 cttcatggtc atgccggcaa tgatcggcgg cttcggcaac tggttcgtgc cgatcatgat
     5401 cggggcgccg gacatggcct tcccgcgcat gaacaacatc agcttctggc tgctgatccc
     5461 gtcgttcatc ctgctcttgg gctcggcgtt cgtcgacggc ggcccgggca acggcgcggg
     5521 aaccggctgg accatctatc cgccgctgtc ctcggccggc agccacgccg gcccggcggt
     5581 cgacatggcg atcttcgcgt tgcacctggc cggcgcgtcg tcgatcctcg gcgcgatcaa
     5641 cttcatcacc accatcttca acatgcgcgc gccgggcatg acgcttcacc gcatgccgct
     5701 gttcgtgtgg tcgatcctgg tgaccgcgtt cctgctgctg ctgacgctgc cggtgctggc
     5761 gggcgcgatc accatgctgc tcaccgaccg caacttcggc actgccttct tcaagtccga
     5821 gctgggcggc gacccggtct tgttccagca cctgttctgg ttcttcggcc accctgaggt
     5881 ctacatcctg atcctgcccg ggttcggcat catcagccag atcgtggcga cgttctcgag
     5941 gaagccgatc ttcggctatc tgggcatggc ctacgcgatg gtcgcgatcg gcgtcgtcgg
     6001 cttcgtggtc tgggcgcacc acatgtacac ggtcggcctc gccgtcgaca cgcgcgccta
     6061 cttcaccgcc gcgaccttga tcatcgcggt gcccacgggc atcaagatct tcagctggat
     6121 tgcgaccatg tggggcggct cgatcgagtt ccgcacgccg atgctgtacg cgatcgggtt
     6181 catcttcctg ttcaccatcg gcggggtgac cggggtggtg ctggcgaatg ccggcatcaa
     6241 catcgcgctc cacgacacct actacgtggt cgctcatttc cattacgtgt tgtctctcgg
     6301 cgccgtgttc gcggtgttcg ccgggttcta ctattggatc ggcaagatga gcggccgcca
     6361 gtacccggag acgctcggca agatccagtt ctggaccatg ttcatcggcg tcaacctgac
     6421 cttcttcccg atgcacttcc tggggctggc cggcatgccg cggcgctaca tcgactatcc
     6481 ggacgcgttc gcgccgtgga acatggtcgc ctcgatcggc tcgtacgtct cgggcgcctc
     6541 ggtgctgctg ttcatgtaca tcgtctggcg caccttcaca acgggcgcgc gctgcgccgc
     6601 caacccgtgg ggcgagggcg cgaccacgct cgaatggcgg acgtcctcgc cgccgccgtt
     6661 ccacacctac gaggagctgc cgcaggtccg ctgatcggcg gcgcggcggg cgggacagac
     6721 caatgagcga gatgtcaagc gcgatcggcg aggcgcccgc ggccggcgcg ggccccgcgg
     6781 acttcgtcgc cctgctcaag ccgcgggtga tgtcgctggt gtgcttcacc gggctcgccg
     6841 gcatgctgct cgcgccggga tcgctccatc cggtgatcgc gttcacggcg ctgctgtgca
     6901 tggcggtcgg cgcgggtgcc gcggcggcga tcaacatgtg gtgcgaccgc gacatcgacg
     6961 cgctgatggc gcgcacgcgg gcgcgcccga tcccggccgg gcgcgtggcc gcggccgacg
     7021 cgctcggctt cggcgtgacg ctcgcggtcg gggcggtggc gacgatgggg ctcgcggtca
     7081 actggacggc ggcggcgctg ctggcgctga ccatcgcgtt ctacgtgttc gtctatacca
     7141 tgtggctcaa gcggcggacc ccgcagaaca tcgtcatcgg cggcgccgcc ggcgcgctgc
     7201 cgccggtgat cggctgggcg gcgatgtcgg gctcggtcac cctcgagccg atgctcctgt
     7261 tcctcatcat ctttctgtgg acgccgccgc acttctgggc gctggcgatc ctcaaggcgg
     7321 aggagtacgg ccgcgccggg gtcccgatga tgccgaatat cgccggcccg gcttcgaccc
     7381 gccgccagat tttcgtctat gcactcgcgg tcgctgcttg cgccttgctg ccggcagccc
     7441 tcggctttgc cggcattgcc tacggcgttg ccgccgctct gctcggcctg gaattcttgc
     7501 gccgcgcctg gcgcctgctt cgagcggacc gcgatctggc ccccgcgcgc cggcttttcg
     7561 gctattcgat tttctatctc ttcgccatct ttgcggcgct gttgatcgaa cgcctgctcg
     7621 tgccggcaac cggggtggcg tcgtgaagcc cgagcgtgtc gaactcaccg aaagggaaaa
     7681 aaaggcgcgt tccgcgcgca atatcgcgat cgccctggcg ctcgccgcct tcgtcatcct
     7741 cctctatgtc gccacatggg ccaagctcgg cgccaatata ttgaaccggc cgctttaggt
     7801 gtttgcgcga tga
//
LOCUS       DASWHU010000373         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2489471, whole genome shotgun sequence.
ACCESSION   DASWHU010000373 DASWHU010000000
VERSION     DASWHU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489471"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VGB88_13895"
     CDS             complement(<1..1652)
                     /locus_tag="VGB88_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9648593.1"
                     /translation="MAADYKDTLFLPRTAFPMKAGLPEREPELLERWQRIDLWGRLRK
                     AAAGHRKFILHDGPPYANGPIHIGHALNKILKDMVNRARQMSGEDAHFIPGWDCHGLP
                     IEWQIEQDYRKSGRSKDDIPVDQFRRECRAYADKWVLVQMAEFQRLGVLADWERRYLT
                     MSYRAEAQIVRELGKFLVGGGLYQGSKPVMWSPVEKTALAEAEVEYHDITSPAIYVRF
                     PVVEASDPALSGADVVIWTTTPWTIPGNRAIAYGAHIEYGVYEVVAVAEGALARPGDR
                     LVFASALEGQVCAKAGLARVERVQTLRGDDLAGTVARHPLYGEHYRIEAPLLAGDFVA
                     TDQGTGFVHIAPGHGEDDFELGRRHGLEVPATVGDDGRFYDHVPLFAGEHVYKVNPVI
                     TAALEERGRLLAREQYVHSYPHSWRSKKPVIFRNTPQWFISMEINGLRAKALAAIDAT
                     RWLPESGHKRIRSMIETRPDWCVSRQRAWGVPITVFVHKHTGEPLRDADVIARVANAV
                     AAGGADVWFSGDASDFLGPGHDPEDYDRVRDILDVWFDSGST"
     gene            complement(1674..>1956)
                     /locus_tag="VGB88_13900"
     CDS             complement(1674..>1956)
                     /locus_tag="VGB88_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX9648594.1"
                     /translation="DTDTDWHPAVASCGVRPTFAGADPVLEVHLFDFDGDLYGRYLRV
                     AFVTWIRAEERFEDAGALVRQMAEDCREARFMLAGARPPRDVGRTAFNQ"
BASE COUNT          283 a          683 c          694 g          296 t
ORIGIN      
        1 tgcgtgctcc ctgagtcgaa ccagacgtcg agaatgtcgc gcacccggtc gtagtcctcg
       61 gggtcgtggc cggggccgag gaagtcggac gcgtcgccgc tgaaccagac gtcggcgccg
      121 cccgccgcca ccgcgttcgc cacgcgcgcg atcacgtccg cgtcgcgcag cggctcgccg
      181 gtgtgcttgt gcacgaacac cgtgatcggc accccccagg cgcgctggcg cgacacgcac
      241 cagtcggggc gggtctcgat catcgagcgg atgcgcttgt gccccgactc cggcagccag
      301 cgggtggcgt cgatcgcggc cagcgccttg gcgcgcaggc cgttgatctc catgctgatg
      361 aaccactgcg gcgtgttgcg gaagatcacc ggcttcttcg accgccacga atggggatag
      421 gagtggacgt actgctcgcg cgcgagcagg cgcccgcgct cctcgagcgc cgcggtgata
      481 acggggttga ccttgtagac gtgctcgccc gcgaacagcg gcacgtggtc gtagaagcgg
      541 ccgtcgtcgc cgacggtggc cggcacctcg aggccgtggc ggcggccaag ctcgaagtcg
      601 tcctcgccgt gcccgggcgc gatgtgcacg aagccggtgc cctggtcggt ggcgacgaag
      661 tcgccggcca agagcggcgc ctcgatccgg taatgctcgc cgtagagcgg gtggcgcgcg
      721 acggtgccgg cgagatcgtc gccgcgcaac gtctgtacgc gctcgacgcg cgcgagcccc
      781 gccttggcgc agacctgccc ctccaacgcg gacgcgaata ccagcctgtc gccgggcctc
      841 gccagcgcgc cctcggcaac cgcaaccacc tcgtagacgc cgtactcgat atgcgcgcca
      901 taagcgatgg cgcggttgcc cgggatggtc cagggcgtgg tggtccagat caccacgtcg
      961 gcgccgctga gcgcgggatc gctcgcctcg accaccggga agcggacata gatggccggc
     1021 gaggtgatgt cgtgatactc gacctcggcc tcggccagcg cggtcttctc gaccggcgac
     1081 cacatcaccg gcttcgagcc ctggtacagt ccgccgccga ccaggaactt gccaagctcg
     1141 cgcacgatct gagcctcggc ccggtaggac atggtcagat accgccgctc ccagtcggcg
     1201 agcacgccga ggcgctggaa ctccgccatc tgcaccagca cccacttgtc ggcataggcg
     1261 cggcactcgc gccggaactg atcgaccggg atgtcgtcct tgctgcggcc cgacttgcgg
     1321 tagtcctgct cgatctgcca ctcgatcggc agcccgtggc agtcccagcc ggggatgaag
     1381 tgggcgtcct cgccgctcat ctggcgggcg cggttgacca tgtccttgag gatcttgttg
     1441 agcgcgtggc cgatatggat cgggccgttg gcgtagggcg ggccgtcgtg gaggatgaac
     1501 ttccggtgcc ccgccgccgc cttgcgcagc cggccccaca ggtcgatgcg ctgccagcgc
     1561 tcgagcagct cgggctcgcg ctcgggcagg cccgccttca tcgggaaggc ggtgcgcggc
     1621 aggaacaggg tgtccttgta gtcggcggcc acgggcgtca tcgcatcgcc aagctattgg
     1681 ttgaaggcgg tccggccaac gtcgcgcggc gggcgcgcgc cggcaagcat gaagcgggcc
     1741 tcgcggcagt cctcggccat ctggcggacc agcgcgccgg cgtcctcgaa cctctcctcg
     1801 gccctgatcc aggtgacgaa cgcgaccctg aggtagcggc cgtagaggtc gccgtcgaag
     1861 tcgaacaggt gcacctcgag caccgggtcg gcgccggcga aggtcgggcg cacgccgcag
     1921 ctcgccaccg ccgggtgcca gtcggtgtcg gtgtcg
//
LOCUS       DASWHU010000374         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_629343, whole genome shotgun sequence.
ACCESSION   DASWHU010000374 DASWHU010000000
VERSION     DASWHU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629343"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VGB88_13905"
     CDS             <1..125
                     /locus_tag="VGB88_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEX9648595.1"
                     /translation="NLIDSLGYRGWVGCEYRPAGATADGLAWAFDYGISGHGGG"
     gene            431..1627
                     /locus_tag="VGB88_13910"
     CDS             431..1627
                     /locus_tag="VGB88_13910"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9648596.1"
                     /translation="MALTARDVEELSRDRGPTARRRVIGEVGRAYANGALSPRARREA
                     LAIARVVLDDVEVVVRQAMAEALCRAPDLPRDLALALAKDVFEVARPILEISRALSDD
                     DLIELIRDPVSRALRARLGLSGEDALAVEAERRRIAVARRQTVSERVAQALIEEGTAT
                     VAATVLANRGATIGEPAIHLAVKRFGTDQGVQSALVDRATLPVTVVERLMYLVSEGLR
                     ASLEQRHTVSPRVLAAIARQVREKATLDLLKGDDGEVRGDRLVAGLERQGRLTPTLIL
                     RAVCTGKIQFAESALAQVTDLPIDAVRSRLRHGSALDVRDLARRAGLPAHFAPVIESL
                     FRRAYVLCGDPSAGSRQWFTEQMTLALVETVGRDAERTRDAGADELDYVLAELERTRA
                     KEAAAR"
     gene            complement(1632..>3125)
                     /locus_tag="VGB88_13915"
     CDS             complement(1632..>3125)
                     /locus_tag="VGB88_13915"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9648597.1"
                     /translation="YDAQLTWDGWAQPSPALGVTTVIIGNCGFAVAPCRARDRDAMMR
                     NLCNVEGMSLEALRAGMRWGFETFPEYLALLEGRGVGPNVAAFCGHSAIRTWVMGEAA
                     NERAARAEEVAAMATLVRGAMADGAVGFATSTFEGHNGEGGVPMPSLLAEDGELAALV
                     GAMGESGGGTFMLTRGSRTTMPFLESLAAQTGRPVVVAALLHDHTRPDAAFAEFDAIA
                     AAQARGHELYGQVSCCPLTMDFTLRNPYLMESYRAWRPAMKAKGEALARVYADGAFRT
                     AMKAELDRAQGLRAFTGDWSRIFVARAADPAHAALEGRDVASLAAEAGEHPLDRFLDL
                     GLAERLETVFSSLLLNSDEDAVARLLADPHSTIALSDAGAHLTFFCDAGFGLHLYGHW
                     VRERALMPLEAAVHQLTARQAAIYRIPDRGRLAVGAHADLMLFDPATIARTANRRVHD
                     LPAGASRLVCAAEGLHGVWVNGARVAAGDGPAAGAPLPGRVIRRFER"
BASE COUNT          422 a         1101 c         1184 g          418 t
ORIGIN      
        1 tcaacctgat cgactcgctc ggctaccggg gctgggtcgg ctgcgagtac cgtcccgccg
       61 gggcgaccgc cgacggcctg gcctgggcct tcgactacgg catcagcggc cacggcggag
      121 gctagagcgg gatgatatta agtcggaacc ctgagcctgt tttggtcgtc accccggact
      181 ccgcgaagcg gatatcccgg gtccatacac ccgtgcaagc ggcgggattg accccggctc
      241 gcggcgctgc gcgccttggc cggggtgacg ggtgatggat gcccatgttt caaatcatca
      301 ggctctcgcc cacgcgtcgg cttgaagcag attgcgcctg tgccatcggc gatattaggt
      361 ttgcccctgg ccgcccgctg gagggaaggg ttcgggctcg gccgacggca aggggggagg
      421 cacgcgcgag ttggcactga ctgcgcggga cgtcgaggaa ttgagccgcg accgggggcc
      481 gacggcgcgc cggcgcgtga tcggcgaggt cggccgcgcc tacgccaacg gcgcgctgtc
      541 gccgcgggcg cggcgcgagg cgttggcgat cgcccgggtg gtgctcgatg acgtcgaggt
      601 ggtggtgcgc caggcgatgg ccgaggccct gtgccgcgcc ccggacctgc cgcgcgacct
      661 cgcgcttgcg ctcgccaagg acgtgttcga ggtggcgcgc ccgatcctgg agatatcgcg
      721 ggcgctgagc gatgacgacc tgatcgagtt gatccgcgac ccggtctcac gggcgctgcg
      781 cgcgcggctc gggctgtcgg gcgaggacgc gctcgccgtc gaggccgagc gccggcgcat
      841 cgccgtggcc cggcgccaga cggtgagcga gcgcgtggcg caggcgctga tcgaggaggg
      901 caccgcgacg gtggcggcga ccgtgctcgc caaccggggc gcgacgatcg gcgagccggc
      961 gatacacctg gcggtcaaac ggttcggcac cgaccagggc gtacagtcgg cgctggtcga
     1021 ccgggcgacg ttgccggtca ccgtcgtcga gcggctgatg tacctggtct cggaggggct
     1081 gcgcgcgagc ttggagcagc ggcacaccgt ttcgccgcgc gtgctcgccg ccatcgcccg
     1141 ccaggtgcgc gagaaggcga ccctcgactt gctgaagggc gacgacggcg aggtgcgcgg
     1201 cgaccgcctg gtggcgggcc tggagcgcca gggccggctg accccgaccc tgatcctgcg
     1261 cgcggtctgc accggcaaga tccagttcgc agagtccgcg ctggcccagg tcaccgacct
     1321 gccgatcgac gccgtgcgca gccggctgcg ccacggctct gcgctcgacg tcagggacct
     1381 ggcgcggcgc gcgggcttgc cggcgcattt cgcgcccgtg atcgaatccc tgttccggcg
     1441 cgcctacgtg ttgtgcggcg atccctcggc cgggtcgcgc cagtggttca ccgagcagat
     1501 gacgctggcg ctggtcgaga cggtgggcag ggacgccgaa cggactcgcg acgccggcgc
     1561 ggacgagctc gactacgtcc tggccgagct tgagcggacg cgcgccaagg aggccgcggc
     1621 gcgctgagcg ctcagcgctc gaaacggcgg atcacccggc cgggcagcgg cgcgcccgcg
     1681 gccgggccgt cgccggcggc gacgcgggcg ccgttgaccc acaccccgtg cagcccctcg
     1741 gccgcgcata ccagccggct tgcgccggcc ggcagatcgt gcacgcgccg gttggcggtg
     1801 cgcgcgatcg tcgccgggtc gaacagcatc aggtcggcat gggcgccgac cgccagccgg
     1861 ccgcggtccg ggatccggta gatcgccgcc tggcgcgccg tgagctggtg gaccgcggcc
     1921 tcgagcggca tcagcgcgcg ctcgcgcacc cagtggccgt agaggtggag cccgaagccg
     1981 gcgtcgcaga agaaggtgag gtgcgcgccg gcatcggaca gcgcgatggt gctgtgcggg
     2041 tcggcgagca gccgggcgac cgcgtcctcg tcggaattga gcagcagcga gctgaacacg
     2101 gtctcgagcc gctccgccag cccgaggtcg aggaaccggt cgagcggatg ctccccggcc
     2161 tcggcggcga ggctcgccac gtcgcggccc tcgagcgcgg catgggcggg gtcggccgcc
     2221 cgggcgacga agatgcggct ccagtcgccg gtgaacgcgc gcagtccctg cgcccggtcg
     2281 agctcggcct tcatcgccgt gcggaaggcg ccatcggcgt agaccctcgc cagggcctcg
     2341 cccttggcct tcatcgccgg ccgccaggcg cggtagcttt ccatcaggta cgggttgcgc
     2401 aaagtgaagt ccatggtcag cggacagcac gagacttggc cgtagagctc gtgcccgcgc
     2461 gcctgcgccg cggcgatggc gtcgaactcc gcgaacgccg catcggggcg ggtatgatcg
     2521 tgcaacagcg ccgccaccac caccggccgg ccggtttgcg cggcgagcga ttcgaggaac
     2581 ggcatggtcg tgcggctgcc cctggtgagc atgaaggtgc ccccgccgct ttcgcccatg
     2641 gcgccaacca gcgccgcgag ctcgccgtcc tcggccagca gcgagggcat cggcacgccg
     2701 ccctcgccgt tgtgcccctc gaaggtcgag gtggcgaagc cgaccgcgcc gtcggccatc
     2761 gcgccgcgca ccagcgtcgc catcgcggcc acctcctcgg cgcgcgccgc gcgctcgttg
     2821 gcggcctcgc ccatcaccca ggtgcggatc gcggaatggc cgcagaacgc cgccacgttc
     2881 gggccgacgc cgcggccctc gagcagcgcc agatattcgg ggaaggtctc gaaaccccag
     2941 cgcatgccgg cgcgcagcgc ctcgagcgac atgccctcga cgttgcacag gttgcgcatc
     3001 atggcgtcgc ggtcgcgcgc gcggcagggg gcgacggcaa agccgcaatt gccgatgatc
     3061 accgtggtga cgccgagcgc cggcgagggc tgcgcccacc cgtcccaggt cagctgggcg
     3121 tcgta
//
LOCUS       DASWHU010000375         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_629493, whole genome shotgun sequence.
ACCESSION   DASWHU010000375 DASWHU010000000
VERSION     DASWHU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629493"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VGB88_13920"
     CDS             <1..983
                     /locus_tag="VGB88_13920"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9648598.1"
                     /translation="EQGEVQLADPVAKFLPAFADMQVSVVETDRFGKVTNTLVPAARP
                     MTVQDLLRHTSGLVYGQLTGNAPVREAYQEAGVYSPEDRGLAIYRDLAPEDEVARLAK
                     APLAHHPGTVWEYGLATDVLGRLVEAASGMRLADFLHERLFVPLGMADTGFWVPADKQ
                     ARIADPFATDPATGVPNQLLDPTVVPANDSGGAGGVSTAGDYLRFAQMLLDGGALDGT
                     RILSRTTVALMASDHLGPDVDTPLGPGQLLLGSPGYTFGLGFSVRTGAGIATVPGSAG
                     EFMWAGYAGTYFWIDPGEQLVGVMMTQAPGPSRASYRRLIKQLTYQAIVD"
     gene            980..>2338
                     /locus_tag="VGB88_13925"
     CDS             980..>2338
                     /locus_tag="VGB88_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648599.1"
                     /translation="MTPSGLRRLVAAAGLLGLGACGWAPPAEVSGALALARIAEHQAR
                     LGDAAGADRALARALQVAESRQEQKDRDAVRFAVAVAEARIGRLAEASNVAATIAEPG
                     ARIAAVLAVGAIALEGGGSEAAAEALAAARTVVDSRPDWADDEILRALDQIALFQAAM
                     GDAAGALATAGRASASAESDPAAVLWLRAMIALIAVETAGEPAALAILAGIERDAAAV
                     EARGEREEIAAALAGVRARAADIDGALEAATATPVLEARAASLAYVAAARAARGDAAG
                     AQATAGEAYRTYLQRPSAGAWFADAAEGMAPDAGAVVDGVDGEGAFAAAGIGVGEVVV
                     QFDGIDVGSADQLEELVGSRPAGTPIEVAVASDAGTRLVRVVLGRADAAEQPTLSVLA
                     AARAASGDVAGAVATADRIADSGDRIATHSMVAAMRASAGDGPGATVAADAACANAER
                     LDA"
BASE COUNT          290 a          843 c          903 g          302 t
ORIGIN      
        1 tcgagcaggg cgaggtgcag ctcgccgacc cggtggcgaa gttcctgccc gccttcgccg
       61 acatgcaggt gagcgtggtc gagaccgacc gcttcggcaa ggtcaccaac acgctggtgc
      121 ccgcggcgcg gccgatgacg gtccaggacc tgctgcgcca cacctcgggc ctggtctacg
      181 gccagctcac cggcaacgcc ccggtgcggg aggcttacca ggaggccggc gtctacagcc
      241 ccgaggaccg gggccttgcc atctaccgcg acctggcgcc cgaggacgag gtcgcgcggc
      301 tggctaaggc gccgctcgcg catcatcccg gcacggtgtg ggagtacggc ctggccaccg
      361 acgtgctcgg ccgcctggtc gaggcggcgt ccggcatgcg cctcgccgac ttcctccacg
      421 agcgcctgtt cgtgccgctc ggcatggccg acaccggctt ctgggtgccc gcggacaagc
      481 aggcgcgcat cgccgatccg ttcgcgaccg atccggccac cggcgtgccc aaccagctgc
      541 tcgatccgac cgtggtgccg gccaacgact cgggcggcgc gggcggggtg tcgaccgcgg
      601 gcgactacct gcgtttcgcc cagatgctgc tcgacggcgg ggcgctcgac ggcacccgga
      661 tcctgagccg gacaacggtt gccctgatgg cctcggacca tctcggcccc gacgtcgaca
      721 ccccgctggg cccgggccag cttttgctcg gctcgcccgg ctacaccttc ggcctcggct
      781 tctcggtgcg caccggcgcc ggcatcgcca cggtgccggg ctccgcgggc gagttcatgt
      841 gggcgggcta cgccggcacc tatttctgga tcgacccggg cgagcagctg gtcggggtga
      901 tgatgaccca ggcgccgggg ccgagccgcg cctcttaccg ccggctgatc aagcagctca
      961 cctaccaggc gatcgtcgat tgaccccaag cggcctccgg cgcctggtcg cggcagcggg
     1021 gctgctcggc ctcggcgcct gcggctgggc gccgccggcc gaggtaagcg gcgcgctcgc
     1081 gctcgcccgc atcgccgagc atcaggcgcg cctaggcgat gccgccggcg cggaccgggc
     1141 tcttgcgcgc gccttgcagg tggcggagtc gcgccaggaa cagaaggacc gggatgcggt
     1201 caggttcgcg gttgcggtcg ccgaggcgcg aatcgggcgg cttgccgagg caagcaatgt
     1261 cgcggccacg atcgccgagc ccggcgcccg catcgccgcc gtgctcgcgg tcggcgcgat
     1321 cgcgctcgag ggcggcggca gcgaggccgc cgcagaggcg ctcgccgcgg cgcggacggt
     1381 cgtcgactcc cgccccgatt gggccgacga cgagatcctg cgcgccctcg atcagatcgc
     1441 gctgttccag gcggcgatgg gcgatgccgc aggggccttg gcgaccgcgg gacgagcctc
     1501 ggcatcggcc gagtcggacc cggcggcggt gctgtggctg cgcgccatga tcgcgctgat
     1561 cgcggtcgag accgcgggcg agccggcggc actggcgatc cttgcaggca tcgaacggga
     1621 tgcggccgcg gtcgaggcgc ggggcgagcg cgaggagatc gcggcggcgc tcgccggagt
     1681 gcgcgcgcgc gcggccgaca tcgacggcgc tctcgaggcc gcgacggcga cgccggtgct
     1741 cgaggcgcgc gccgcgagcc tcgcctacgt cgccgcggca cgggcggcac gcggggacgc
     1801 cgcaggcgcg caagccacgg cgggcgaggc gtatcggacc tatctccagc gcccgtcggc
     1861 gggggcgtgg ttcgccgacg cggccgaagg catggcgccg gacgcgggcg cggtggtcga
     1921 tggcgtcgat ggcgaaggcg cgttcgccgc ggccggcatc ggcgtcggcg aggtcgtcgt
     1981 ccaattcgac ggcatcgacg tcggctcggc cgatcagctc gaagagctgg tcgggtcaag
     2041 gccggccgga accccgatcg aggtcgcggt cgcgagcgat gcggggacgc gccttgtgcg
     2101 cgtcgtcctg ggtcgggcgg acgcggccga gcagccgaca ttgtccgtgc tcgcggccgc
     2161 gcgcgcggcg agcggagacg tggccggagc ggtcgcgacc gccgaccgga tcgccgattc
     2221 gggtgaccgg atcgcgaccc attccatggt cgcggccatg cgggcttcag ccggcgacgg
     2281 gcccggcgcg acagtcgcgg ccgatgccgc ctgcgccaat gccgagcgcc tcgatgca
//
LOCUS       DASWHU010000376        13291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1009544, whole genome shotgun sequence.
ACCESSION   DASWHU010000376 DASWHU010000000
VERSION     DASWHU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009544"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VGB88_13930"
     CDS             <1..819
                     /locus_tag="VGB88_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648600.1"
                     /translation="VGLDALGTIMAHVQQPFTWPNLPDGEVVRAIFAKYAMLPFVIPT
                     PPLRTMLDTTTTQRDNDAVYVRQLALRNAYEYYVQPDPVIGRDVGHFHPSLIHVPPQG
                     VLSVDFGTQTNLIDLSVANDMLAPTSVVAVSSDPRTRAPIPAIAPASTEPPMGLEPTL
                     NRIIPPPVERPAGTDAASPAEVQAQAFARATNSSRAVRANGTVDGLKYNRPLLAGLPV
                     LIRGGGRQYSGLYYVTSVTHRISRDDYTQAFSAWRNAVGLTGAEVFIDPLSAVA"
     gene            834..1544
                     /locus_tag="VGB88_13935"
     CDS             834..1544
                     /locus_tag="VGB88_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX9648601.1"
                     /translation="MPDATARMPDVATRERVYGKYRGVVLNTTDPLNLGRIQASVGEV
                     LGEVPTGWATPCAPYAGTGAGLFSVPPVGAGVWIEFEGGDVSRPIWSGCYWGAGEVPL
                     NPPGAPSLPTTKIWRSDFGLTAVLDDAAQTVTLSDAAATNQVIVSVPTGTVTVKGLAR
                     VVLEGPLIQEGSQSAAHPAVFGDRLLTYLGQLVGMFNAHVHPGELAGGFLPVTPAPPV
                     APMPPPTPSLLSIKVLLE"
     gene            1555..1854
                     /locus_tag="VGB88_13940"
     CDS             1555..1854
                     /locus_tag="VGB88_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648602.1"
                     /translation="MAIEPGRFTTNPTDYSQFVNSMAAEIEAQLNDLLDLDDLPALPT
                     GADDREVRDRRRLFVAIARAVVIHLNDRRLSINIEVPDGIGGTVTVHPTFDIDGI"
     gene            1855..2232
                     /locus_tag="VGB88_13945"
     CDS             1855..2232
                     /locus_tag="VGB88_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX9648603.1"
                     /translation="MPADPTYLDFPLAIDARGRTATTGDDDHVRDLILQVLFTSPGER
                     VNRPEFGCGLKQLVFAPASDALAAATQQLVQGALIRWLDHVIAVEQVQVVAVEASIEA
                     TVVYQRRDTGERREEIFRHPVPA"
     gene            2240..4852
                     /locus_tag="VGB88_13950"
     CDS             2240..4852
                     /locus_tag="VGB88_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648604.1"
                     /translation="MAVSRSGLIWCQDERRSGEVRDPAHLLNGIDFVEYRRDLAALPA
                     RRHRLEVTYLKAPVVALQTDAASYAVTGGVRVVGIRVLSVEPDAGDALRVIAFLDREG
                     DFSTYILAVAHASLDPELSEARFGFKASCPTELDCRIRVDCPPEALAEPALDYLAKDY
                     QSFRRLMIDLIPERNPGWHERLPADLGIALVELFAYAGDYLSYQQDAAVTEGYLDTCL
                     HRVSAARHARLVDWRMHDGRNAFTFVHFAVDGGTDGVVPAGARLLTRVGVPLRGQPAA
                     PGVVIPPAAVDFDGDAALAEVTVFETTATARAVDAHNELVIHDWGNAECCLAKGARQA
                     FLYGLPATGANPSAFRPELEAGDYLLLEERLSPVTGLAADADPDRRQVVRIVEVEDTV
                     DPAYRMVFVGRALTPRAGAGQQPLPLQRLVWQEQDALAFTLCLSAETPETGPIAQVSV
                     ARGNVAPADHGRTVVRELPPPSPGAGRWPLPALDLPDAPLSHQPMPAAPDYADDGRLV
                     QGRHDLARDVREVMPAAVLELHFPGGEDERWEPVPHLLDSTPFDQHFIAEVDDGGVAR
                     LRFGDDQYGRRPLDAESATARYRIGNGRAGNIGAGALVHVIAPTAAELIDPADPLAPP
                     ANFAAVARVYQPLAARLGTDPETIEEVRQLAPEAFRAVQFRAVTEADWAEVALRHPAV
                     AAAKARFRWTGSWHTVFVAIHPRDADDLERLPGGGAALTASFAAAMRAHLRRFKLAGY
                     DLAVRAAVYVPLEIEIEICVARGHFRGDVLAAVGEALSNRAFADGGRGFFHALNFGFG
                     QPVYLSRLYAAVEAVEGVDSAKVLVFKRYWEVARDELERGLIEMGVVEIARLDNHLSA
                     PEYGVLRLLTVGGL"
     gene            4855..8337
                     /locus_tag="VGB88_13955"
     CDS             4855..8337
                     /locus_tag="VGB88_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648605.1"
                     /translation="MIDIPDARFCACCQAPAPRAPAEIWNRPSLSRLAFRIGSFATFR
                     QAMLQAIAREPALAGLATRESDDYAITLLELFAAVGDVLTFYSERYVNELYLGTARER
                     DSILRMVRLIGYQLRPGLAATTMLDFTLDDDALTRIRKGLKVMSVPGQDERAQTFETI
                     EAMAADARLNALDVFAPPVLFNAFHQGRAEAPVTARPDPLGPGDRFVVFGLSRIEEKT
                     VAGLDLRPDGEHLRFAPPIQGANLWVEVARAAKRRRRLRFFGHDAPDSHQRYNDDHTV
                     PPQNRWQSVPIPHTFAENVTSYPLDTRYDDLKPGAQLLIDAGPSAAPRLRTALVVNTE
                     DLPATQGAVTDTVTHATLRQTLRDRPAAVALGGGARRVFARSGAGHVLRIDPAATSRW
                     SDFGGFVATADLSAVATGPAQIDLFTRDGLGHLRHAAWSGGAWGAFADRGGILTTGPA
                     ALAVGGEVFVFVRGLDLGLWYLRISGGAAGAWTARGGILTSPPVPVTSDGTTIDVFVR
                     GLGRGLWRQHWTGAAWSEWEPLAGTLATAPAAASTGAGRLDVVALDDDGALIHRRTTG
                     GDWTEWATLGGAAEGTPAILATGPDRVDVFVRRPNGELWQIARTGAAWSSWVSLGGSL
                     ASSPTVALSGGTLRVFARGADGTLVTRARTGSVWSGWSALGDGLGAIPDRRATRIYEI
                     ETPDIGFRGYDYPREISAGRVVARLAAAPGLGALDKGRRILLDDGASEHLARVTATRL
                     VSSELGAAPDHLAVDFEPALPAPLGAAVLRGNIAEASHGETQPEEPLGHGDAAKAFQR
                     FTLSRKDLTYLPSETSLAGEAELEMRVNGELWHEVPSLYARAPGERAYTARRTDEGET
                     VVTFGDGRTGARVPSGAMNVVARYRTGIGLEGRVKADQLSILLERPVGLRAVTNPLPA
                     TGAADPETRDDARAAAPTTVRTFGRAVSEADFACLAESSGLVKRAHATWVWRRLEKTL
                     HLTVAGEDGARLTEGELATLESALDAARDPNRPLLLANLVRVPLVVRAKLLRDSAFKA
                     DDVEATARAAIEAHFAFEAMPLGQAVHASAIYAVLQGARGVVAVDLDLFHLKDHADLS
                     ADERAVRAVTADPVQAHIRIFPARPTPGDPADIDRYARAGFDGTMPPPVLAAEQAYVA
                     DPAGDIVLTVVESL"
     gene            8339..10606
                     /locus_tag="VGB88_13960"
     CDS             8339..10606
                     /locus_tag="VGB88_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648606.1"
                     /translation="MAFSNANEDKLYGLLPAYLRERDAAEGEPLHALLRIVERQTDAL
                     EGDIGQLYNDAFIETCEPWVVPYIGQLVGTTPLFDDSRVRDGDAARELFPDLRGPSFR
                     PPIALRNRADVAKTIYYRRRKSTLPMLEELARDVTGWAAHAVEFFELLGWTQWLRNHV
                     RPHALRTPDIRSFERMDRLDGAFDEICHTVDVRPISQHEGWHNIPNIGFFLWRLGSYR
                     MEAVAARRLGAAGDFRYHFSPLGNSAPLFSRLRREGDETGLATELHVPQAIRPARFFE
                     DLRAWHALAAPPNHTEFYGLFNDFAGQAAAPAASLMVIVDGVAVAPARVVCRNLADWG
                     QPALDDIAVDVARGRLALGPDYLPADLVEVYFHYGFPADLGGGPYRRRAWLVRRDLAE
                     LMLTVDGTGDPGTHATIAAALADWAAAGRPDCIVRIGDNRTYVEALTIEPADGSFLAI
                     EAADGARPHLRLTAPLTVTGAHPDATLTLGGLLVEGRIEIEGEFGRLRLLHTTLVPGA
                     SIAEPDPDLPPPPPAPVEPSIRAAGTNVDGDVINSELRVELAFSVTGPIRLPAHSETL
                     VAFDSIIDGVGEAAIAGVGAADAIGPALRLERVTVRGETHVRQIDLASEVIFDGLAVA
                     ERVQVGCVRFSYVVPGSATPRRYRCQPDRAELKAIDAAEAQAGPLSPAERAAIRDRAR
                     RRVRPEYAAESYGQPDYLQLSLGGPLEIATGAEDGSEMGAYSHLKQPQREANLRLRLE
                     EYLPFGLEAGFIYVT"
     gene            10650..>13291
                     /locus_tag="VGB88_13965"
     CDS             10650..>13291
                     /locus_tag="VGB88_13965"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX9648607.1"
                     /translation="MAGDYTRFTFDPRKRYSGVLMQQGRVQLDSDWNEEVAIVNRRVR
                     TLALDTFGPVGLPLLTTPDAFLIGLLGGAPADLSIGAGRLYVDGWLAEHFAGEDFTYL
                     NQPFLPAPPDLPGQGDVVVYLDVWQREVTYIEDPELLDAALGGVDTATRRQTVWQVKF
                     DARDGAECGMDVGDPPSAGRLSSEAIAPPAPDDPCILPPLAGYRGLENRLYRVEVHDG
                     GPLGTARFRWSRDNGSIVSAVTDLQVSGGQTTLTVTRIGRDPVLRFRIDDWVSVTDDH
                     RELYEETPEMAQVVDIDETNRRIVLDRALPTGTGRAFAATPGELEDRRTRIQRWDQTA
                     ALNPLDSDGLITTAAGPIDIEEGIRISFTVDPAGGAFRNGDYWVFAARTADASVEELD
                     EAPPRGIHHHYVQLAAITGLGGANPQVSDCRPRPEDGVAQCCCCLVTVGARDDQRADF
                     FDLAGAIAALPNLAPNDSVPVIVCLLDGDHALGTPVVVTRPRVTIRGCGWASRLLPTQ
                     GPALSLRGVEQAVEDLAIFAEQTPPLIDAAGQQLRIEGCRLENQASGPAVLARRVLDL
                     KILRNLVLAQGGGLDLAGDLIEVAGNRILGGPVRVREPSDTVDIRDNDLLGAIEDAVV
                     LGGRGIVYEIDVAHNRIRGALRHGIASGFFDPENQGGDGIVAGLRILGNDIAECIGPN
                     TQRTVNDPPFAGIALGRVYDLVARDNRIERNGEQATGPVCGIYVRHSRGVEIDRNIVR
                     MNGRQPDGTAIPGVQAGISLRDATVMLSETPDPAAANRSIAELGIMPAAKITGNLVDS
                     RRGPALSIFGQGPMMIEGNRFQAIDVLSDLSDLTFSTVDQYVGTVFVYNSGLPAYFSG
                     FLSGAGMNALTTGDVAALSGSPLQT"
BASE COUNT         1828 a         4878 c         4652 g         1933 t
ORIGIN      
        1 gtcggcctcg atgcgctcgg cacgatcatg gcgcatgtcc agcagccctt cacctggccc
       61 aacctgccgg acggcgaggt ggtgcgcgcg atcttcgcga agtacgcgat gctgccgttc
      121 gtgataccga cgccgccgct gcgcacgatg ctcgatacca ccacgactca gcgcgacaac
      181 gacgcggtct atgtacgcca gctcgcgttg cgcaatgcct acgagtacta cgtccagccc
      241 gaccccgtga tcggccgcga cgtgggccat ttccacccct cgctcatcca cgtgccgccg
      301 cagggcgtgc tgtcggtcga tttcggcacc cagaccaact tgatcgacct tagcgtcgcg
      361 aacgacatgc tggcgccgac ctcggtggtt gcggtgtcgt ccgatccgcg tacgcgtgcg
      421 ccgatcccgg ccatcgcgcc ggcctcgacc gagccgccca tggggcttga gccgaccctc
      481 aaccggatca tcccgccgcc ggtcgagcgg cccgcgggca ccgacgcggc gagcccggcc
      541 gaggtccagg cgcaggcgtt cgcgcgcgcg acgaactcca gccgcgcggt gcgtgccaac
      601 ggcaccgtcg acggcctcaa atataaccgg ccgttgctcg ccggcctgcc ggtgctgatc
      661 cgcggcggcg ggcgccagta cagcggcctt tactacgtca ccagcgtcac ccatcgcatc
      721 tcgcgcgacg actacaccca ggccttctcg gcctggcgca acgcggtcgg gctgaccggg
      781 gccgaggtgt tcatcgaccc gttgtcggcg gtcgcctgag gaggagacga gccatgcccg
      841 acgccactgc gcggatgccg gacgttgcca cccgcgagcg ggtctacggc aagtaccggg
      901 gcgtcgttct caacaccacc gacccgctca acctcggccg catccaggcg tcggtcggcg
      961 aggtgctggg cgaggtaccg accggctggg caacgccctg cgcgccctac gctggcaccg
     1021 gcgccggctt gttctcggtg ccgccggtcg gcgccggggt gtggatcgag ttcgagggcg
     1081 gcgacgtgtc gcggccgatc tggagcggct gctactgggg ggcgggcgag gtgccgctga
     1141 acccgccggg cgcgccgagc ctgccgacga ccaagatctg gcgcagcgac ttcggcctga
     1201 cagcggtcct cgacgacgcc gcgcagaccg tcaccctgtc ggacgcggcg gccaccaatc
     1261 aagtgatcgt cagcgtgccg accggcacgg tcaccgtgaa gggcctggcg cgcgtcgtgc
     1321 tcgagggccc tctgatccag gagggcagcc agtccgccgc ccacccggcg gtattcggcg
     1381 accgcctgct cacttatctc ggccagctgg tcgggatgtt caacgcccac gtccacccgg
     1441 gcgagctcgc cggcggcttc ctgcctgtga ccccggcgcc gccggtggcg ccgatgccgc
     1501 cgccgacccc cagcctgttg tcgatcaagg tcctgctcga atagggaggc gcgaatggcg
     1561 atcgagcccg gcagatttac caccaacccg accgactaca gccagttcgt caattcgatg
     1621 gcggcggaga tcgaggcgca gctcaacgac ctgctcgacc tcgacgacct gccggcgctg
     1681 ccgactggcg cggacgaccg ggaggtacgc gaccgccggc gcctgttcgt ggcgatcgcg
     1741 cgcgcggtgg tgatccacct caacgaccgc cggctgtcga tcaacatcga ggtgcccgac
     1801 ggcatcggcg gcaccgtcac cgttcatccg accttcgaca tcgacgggat ctagatgccg
     1861 gcggacccca catacctgga tttcccgctg gcgatcgatg cgcgtgggcg cacggcgacc
     1921 accggcgacg acgaccatgt gcgcgacctg atcttgcagg tcctgttcac cagccccggt
     1981 gagcgcgtca atcggccgga gttcggctgc gggctcaagc agcttgtctt cgccccggcg
     2041 tcggacgcgc tggcggcggc gacgcagcag ctggtgcagg gcgcgctgat ccgctggctc
     2101 gatcacgtga tcgcggtcga gcaggtgcag gtggtcgcag tggaggcgag catcgaggcg
     2161 acggtggtct atcaacggcg cgataccggc gagcggcgcg aagagatctt ccgccacccg
     2221 gtgccggcgt gagcaggcga tggcggtctc gcgcagcggg ctgatctggt gccaggacga
     2281 gcgccgctcg ggcgaggtgc gcgacccggc gcacctgctc aacggcatcg acttcgtcga
     2341 gtatcggcgc gacttggcgg ccttgcctgc gcgccgccac cgcctggagg tgacgtacct
     2401 caaggcgccg gtggtggcgt tgcagaccga tgccgcgtcg tacgcggtca cgggcggcgt
     2461 gcgcgtggtc ggcattcgcg tgctctcggt cgagcccgac gcgggcgatg cgctgcgggt
     2521 gatcgccttc ctcgaccgcg agggcgactt ctcgacctac atcctcgccg tcgcgcacgc
     2581 ctcgctcgac cccgagctgt ccgaggcccg cttcggcttc aaggcctcgt gcccgaccga
     2641 gctcgactgc cgcatccgcg tcgactgccc gcccgaggcg ctggccgagc ccgcgctcga
     2701 ctatctcgcc aaggactatc agagcttccg taggctgatg atcgacctga ttcccgagcg
     2761 caacccgggc tggcacgagc ggctgcccgc tgacctggga atcgcgctgg tcgagctgtt
     2821 cgcctatgcc ggcgactatc tgtcctacca gcaggacgcg gcggtgaccg agggctatct
     2881 cgacacctgc ctgcaccgcg tgtccgccgc gcgccacgcg cgcctggtcg actggcgcat
     2941 gcatgacggg cgcaacgcct tcaccttcgt gcacttcgcg gtcgacggcg gtaccgatgg
     3001 tgtggtgccc gccggggccc ggctgttgac tcgcgttggc gtgccgttgc gcgggcagcc
     3061 cgcggcgccc ggagtggtga tcccgcccgc cgcggtcgat ttcgacggcg acgccgcgct
     3121 cgccgaggtc accgtgttcg agaccacggc caccgcccgc gccgtggatg cgcacaacga
     3181 gctggtcatc cacgactggg gcaacgcaga atgctgcctc gccaaagggg cccgccaggc
     3241 gttcctctat ggcctgcccg cgaccggcgc caacccgagc gcattccgac ccgagcttga
     3301 ggccggcgac tacctgttgc tcgaggaacg gctgagcccg gtgaccggcc tcgccgccga
     3361 cgccgatccc gaccgccgcc aggtggtgag gatcgtcgag gtcgaggaca ccgtcgatcc
     3421 ggcctaccgc atggtgtttg tcggtcgcgc gttgacgccg cgcgcgggcg ccggccagca
     3481 accattgccg ttgcaacggc tggtctggca agagcaggac gcgctcgcct tcaccctttg
     3541 cttgtcggcc gagacgccgg agaccggccc gatcgcgcag gtctccgtcg cccgcggcaa
     3601 cgtcgcaccc gccgaccacg ggcgcacggt ggtccgcgag ctgccgccgc cgtcgccggg
     3661 cgcggggcgt tggcctttgc ccgcgctcga cctgccggat gcgccgctca gccatcagcc
     3721 gatgccggcg gcgccggatt atgccgacga cgggcgcctg gtccagggcc gccacgatct
     3781 cgcgcgcgac gtgcgcgagg tgatgcccgc ggccgtgctc gagctgcatt tcccgggcgg
     3841 cgaggacgag cgctgggagc cggtgccgca cctgctcgac agcacgccgt tcgaccagca
     3901 cttcattgcc gaggtggacg acggcggggt ggcgaggctg aggttcggcg acgatcagta
     3961 cgggcgccgg ccgctcgacg ccgaatcggc gaccgcgcgc taccggatcg gcaatggccg
     4021 cgccggcaat atcggcgccg gcgcgctggt gcatgtcatc gcgccgaccg cggccgagct
     4081 gatcgatccg gcggacccgc tggcgccgcc tgcgaacttc gccgcggtgg cgcgcgtcta
     4141 tcaaccgctc gccgcgcgcc tcggcaccga tcctgaaacc atagaagagg tgcgccagct
     4201 cgcgccggag gcgttccgcg ccgtccagtt ccgcgcggtc accgaggcag actgggcgga
     4261 ggtcgcgctc cgccatcctg cggtcgctgc ggcaaaggcg cgcttccgct ggaccggcag
     4321 ttggcacacg gtgttcgtcg ccatccaccc gcgcgacgcg gacgacctcg agcgcctgcc
     4381 cggcggcggt gccgcgctga ccgcgtcgtt cgccgccgca atgcgggcgc atctgcgccg
     4441 gttcaagctc gccggctacg acctcgcggt gcgcgccgcg gtatatgtgc cgctggagat
     4501 cgagatcgag atctgcgtcg cgcgcggcca cttccgcggc gacgtgctgg cggcggtggg
     4561 cgaggcgctg tcgaaccgcg ccttcgccga cggcggccgc ggcttctttc acgcgctcaa
     4621 cttcggcttc ggccagccgg tctacctgtc gcgcctgtac gccgcggtcg aggcggtcga
     4681 gggtgtcgac tcggccaagg tcctggtctt caagcgctac tgggaggtcg cgcgcgacga
     4741 gctcgagcgc ggcctgatcg agatgggcgt ggtcgagatc gcgcgcctcg acaatcattt
     4801 gagcgcgccc gagtacggtg ttctgcgcct gctgacggtc ggggggctgt agcggtgatc
     4861 gacatacccg atgcccggtt ctgtgcctgc tgccaggcgc cggcgccgcg cgcgccggca
     4921 gaaatctgga accggcccag cctgtcgcgc ctcgccttcc gcatcggtag cttcgcaacc
     4981 tttcgccagg ccatgctgca ggcgatcgcg cgcgagccgg cgctggcggg cctcgccacc
     5041 cgcgagagcg acgactacgc catcaccttg ctcgagctgt tcgccgctgt cggggacgtg
     5101 ctgaccttct acagcgagcg ctacgtcaac gagctgtact tgggcacggc gcgcgagcgg
     5161 gactcgatcc tgcgcatggt gcgtttgatc ggctaccagc tgcggccggg gctcgcggcg
     5221 acgacgatgc tcgacttcac cctcgacgac gacgcgctca cccgcatccg caagggcctc
     5281 aaggtgatga gcgtgccggg ccaagacgag cgcgcgcaga ccttcgagac gatcgaggcg
     5341 atggccgcgg acgcgcggct gaacgcactc gacgtgttcg cgccgccggt cctgttcaat
     5401 gccttccatc aggggcgcgc cgaggcgccg gtcaccgccc ggcccgatcc gctggggccg
     5461 ggagatcgct tcgtcgtgtt cgggctgtcg cgcatcgagg agaagaccgt cgccggcctc
     5521 gacctgcggc ccgacgggga gcacctgcgt ttcgcgccgc cgatccaggg tgcgaacctg
     5581 tgggtcgaag tcgcgcgcgc cgccaagcgc cgccgccgtc tcaggttctt cggccacgac
     5641 gctccggaca gccaccagcg ctacaacgac gatcacaccg tcccgccgca gaatcgctgg
     5701 cagagcgtgc cgatccctca caccttcgcc gagaacgtca ccagctatcc gctggatacg
     5761 cgctacgacg acctcaagcc cggcgcgcag ctcctgatcg acgccgggcc gagcgccgcg
     5821 ccgcggctgc gcaccgcgct ggtcgtcaac accgaagacc tgcccgcgac ccagggcgcg
     5881 gtgaccgaca cggtcaccca cgcgaccctg cgccagaccc tgcgcgaccg gcccgcggcg
     5941 gtcgcgctcg gcggcggcgc gcgccgggtg ttcgcgcgca gcggcgccgg ccacgtgctg
     6001 cgcatcgacc cggcggcgac ctcgcgctgg agcgacttcg gcggcttcgt cgccaccgcc
     6061 gatctgtccg ccgtcgccac cgggccggca cagatcgacc tattcacgcg cgacgggctc
     6121 gggcacctgc gccacgccgc ctggagcggg ggcgcgtggg gcgcgttcgc cgaccgcggc
     6181 ggtatcctga ccaccggccc ggcggcgctc gccgtcggcg gcgaggtgtt cgtgttcgta
     6241 cgcggcctcg acctcggcct ctggtatctg cgcatcagcg gcggcgctgc cggcgcctgg
     6301 accgcgcgcg gcggcatcct cacctcgcca ccggtgccgg tcaccagcga cggcaccacc
     6361 atcgacgtgt tcgtgcgcgg cctcggtcgc gggctgtggc gccagcactg gaccggcgcc
     6421 gcgtggagcg agtgggagcc gctcgccggc acgctggcca ccgcgccggc ggccgcaagc
     6481 accggcgccg ggcggctcga cgtggtcgcg ctcgacgacg acggcgcgct gatccaccgc
     6541 cgcaccaccg gcggcgactg gaccgagtgg gcgactctcg gcggtgcggc cgaaggcacg
     6601 ccggccattc ttgccaccgg ccccgaccgg gtcgacgtgt tcgtgcgccg cccgaacggc
     6661 gagctgtggc agatcgcgcg caccggcgcg gcctggagca gctgggtgtc gctcggcggc
     6721 agcttggcgt cgtcgccgac cgtggcgctc agcggcggca cgctgcgcgt ctttgcacgc
     6781 ggcgccgacg gcaccttggt cacgcgcgcg cgcaccggat cggtgtggag cggctggtcg
     6841 gcgctcggcg acggcctcgg cgccattccc gaccggcggg cgacgcgcat ctacgagatc
     6901 gagacgcccg acatcggctt ccgcggctac gactacccgc gcgagatttc ggccggccgg
     6961 gtggtcgccc ggcttgccgc ggcacccggg ctcggcgctc tcgacaaggg ccgccgcatc
     7021 ctgctcgacg acggcgcgag cgagcacctg gcacgcgtca ctgcgacgcg gctggtgtcg
     7081 tcggagctcg gcgcggcgcc cgatcatctc gccgtagact tcgagccggc gctgcccgcg
     7141 ccgctcggcg cggctgtgct caggggcaac atcgctgagg cgagccacgg cgagacccag
     7201 cccgaggagc cgctcggcca cggcgacgcc gccaaggcgt tccagcgctt caccttgagc
     7261 cgcaaggatc tcacctactt gccgagcgag accagcctgg ccggcgaggc ggagctcgag
     7321 atgcgcgtca acggcgagct gtggcatgag gtgccgagcc tgtacgcgcg cgcgccgggc
     7381 gagcgcgcct acaccgcacg gcgcaccgac gagggcgaaa cggtagtgac cttcggtgac
     7441 gggcgcaccg gcgcgcgggt gccgagcggc gccatgaacg tggtcgcgcg ctatcgcacc
     7501 ggcatcggcc tcgagggccg cgtcaaggcc gaccagctct ccattctgct ggaacgcccg
     7561 gtcggcctgc gcgcggtcac caatccgctg cccgccaccg gcgccgccga ccccgagacg
     7621 cgcgacgatg cccgcgccgc ggcgccgacc acggtgcgca ccttcggccg ggcggtgtcc
     7681 gaagccgact tcgcctgtct cgcggaaagt tccggcctgg tcaagcgcgc gcacgccacc
     7741 tgggtgtggc ggcggctcga gaagacgctg cacctgacgg tcgccgggga ggacggcgcg
     7801 cgcctgaccg agggcgagct agcgacgctc gagtccgcgt tggacgccgc gcgcgatccc
     7861 aaccggccac tgctgctcgc caacctggtg cgggtgccgc tggtggtgcg ggcgaagctg
     7921 ctgcgcgact cggcgttcaa ggccgacgat gtcgaggcca cggcgcgggc tgcgatcgag
     7981 gcgcactttg cgttcgaggc catgccgctt ggccaggcgg tgcacgcgag cgcaatctat
     8041 gcggtgctcc agggcgcgcg cggggtggtg gcggtcgacc tcgacctgtt tcacctcaag
     8101 gaccacgcgg acctcagcgc cgacgagcgc gcggtgcgcg cggtcaccgc cgatcccgtc
     8161 caggcgcaca tccgcatctt ccccgcgcgc ccgacgccgg gcgaccctgc cgacatcgac
     8221 cgctacgccc gcgccggatt cgacggcacg atgccgccgc cggtgctcgc cgccgagcag
     8281 gcttacgtcg ccgacccggc gggcgacatc gtgctgaccg tggtggagag cttgtgacat
     8341 ggccttctcg aacgccaacg aggacaagct ctatggcctg ctgccggctt acttgcgcga
     8401 gcgcgacgcc gccgagggcg agccgttgca cgcgctgctg cgcatcgtcg agcgccagac
     8461 cgacgccctc gagggcgaca tcggccagct ctacaacgac gccttcatcg agacctgcga
     8521 gccgtgggtg gtgccctata tcggccagct ggtcggcacc acgccgctgt tcgacgacag
     8581 ccgcgtgcgc gacggcgatg cggcgcgcga gctgttcccc gacctgcgcg gcccgagctt
     8641 caggccgccg atcgcgctgc gcaaccgcgc cgacgtcgcc aagaccatct actaccgccg
     8701 gcgcaagagc accttgccga tgctcgagga gctggcccgc gacgtcaccg gctgggcggc
     8761 gcatgcggtg gagttcttcg agctgctcgg ctggacccag tggctgcgca accatgttcg
     8821 cccgcacgcg ctgcgcaccc ccgacatccg ctcgttcgag cgcatggacc ggctcgacgg
     8881 cgccttcgac gagatctgcc acacggtcga cgtgcgtccg atctcgcagc acgaaggttg
     8941 gcacaacatc cccaacatcg gtttcttcct gtggcggctc ggcagctatc ggatggaggc
     9001 ggtggcggcc cggcggctcg gcgccgccgg cgatttccgt taccacttca gcccgcttgg
     9061 caactcggcg ccgctgttca gccggctcag gcgcgagggc gacgagaccg ggctcgccac
     9121 cgagctgcac gtgccccagg cgatccgccc ggcgcgcttc ttcgaggacc tgcgcgcctg
     9181 gcatgcgctc gccgcgccgc ccaaccacac cgagttctac ggactgttca acgacttcgc
     9241 cggccaggcc gcggcgccgg ccgcgagcct gatggtgatc gtcgacggcg tcgcggtggc
     9301 gccggcgcgc gtggtgtgcc gcaacctcgc cgactggggg cagcccgcgc tcgacgacat
     9361 cgccgtcgac gtcgcgcgcg ggcggctcgc gctcggcccc gactacctgc ccgcagacct
     9421 ggtcgaggtc tacttccatt acggatttcc cgccgacctg ggcggcgggc cctatcgccg
     9481 gcgcgcgtgg ctggtgcggc gcgacctggc cgagctcatg ttgacggtcg acggcacggg
     9541 cgatcccggc acgcacgcga ccatcgcggc cgcgctcgcc gactgggcgg cggccggcag
     9601 gcccgactgc attgtccgca tcggcgacaa ccgcacctat gtggaggcgc tcacgatcga
     9661 gcccgccgac ggcagcttcc tcgccatcga ggccgcggac ggcgcgcgcc cgcatctccg
     9721 cttgaccgca ccgctcaccg tgaccggcgc gcatccggac gcgaccttga ccctcggcgg
     9781 gctgctggtc gaggggcgga tcgagatcga gggcgagttc ggccggctgc gcctgctgca
     9841 caccacgctg gtgcccggcg cctcgatcgc cgagcctgat ccggacctgc cgccgccgcc
     9901 gccggcgccg gtcgagccga gcatccgcgc ggcgggcacc aatgtggatg gcgatgtcat
     9961 caactccgag ctcagggtcg agctcgcgtt ctcggtcacc gggccgattc gcctgccggc
    10021 gcacagcgag acgctggtcg ccttcgattc gatcatcgac ggcgtcggcg aggcggcgat
    10081 cgccggggtg ggggcggcgg acgcgatcgg tccggcgctc aggctcgagc gggtcacggt
    10141 gcgcggcgag acccatgtgc gccagatcga ccttgccagc gaggtgatct tcgacggtct
    10201 cgccgtcgcc gagcgcgtcc aggtcggttg cgtgcgcttc tcctacgtgg tgccggggtc
    10261 ggccacgccg cggcgctacc gctgccagcc cgaccgcgcc gagctcaagg ccatcgacgc
    10321 ggccgaggcc caggccggcc cgctcagtcc ggcggagcgc gcggccatcc gcgaccgcgc
    10381 ccggcgccgg gtgcgacccg aatatgccgc cgagagctat ggccagccgg actatctgca
    10441 gctcagcctc ggcggcccgt tggagatcgc gaccggcgcc gaggacggct cggagatggg
    10501 cgcctattcg catctcaagc agccacaacg ggaagcgaac ctgcgcctgc gactcgagga
    10561 gtacctgccg ttcggcctcg aggccgggtt catctacgtg acgtgacatg agcggggctg
    10621 agctccgccc cacagggtga ggattggtca tggcgggcga ttacacgcgg ttcacgttcg
    10681 atcctcgcaa gcgctactcg ggcgtgctca tgcagcaggg gcgcgtccag ctcgattccg
    10741 actggaacga ggaggtggcg atcgtcaacc ggcgggtgcg cacgctggcg ctcgacacct
    10801 tcggcccggt cgggctgccg ctgctgacca cgccggacgc gttcctgatc gggctgctcg
    10861 gcggcgcgcc ggcggacctg tcgatcggcg ccgggcggct ttatgtcgac ggctggctgg
    10921 ccgagcactt tgccggtgag gacttcacct acctcaacca gccctttctg cctgcgccgc
    10981 cggacttgcc ggggcagggc gacgtcgtcg tttacctgga cgtctggcag cgcgaggtca
    11041 cctacatcga ggacccggag ctgctcgatg cggcgctcgg cggcgtcgac accgccaccc
    11101 gcaggcagac ggtctggcag gtcaagttcg acgcgcgcga cggcgccgag tgtggcatgg
    11161 acgtgggcga tccgccctcg gcgggacgcc tgtcgagcga ggcgattgcg ccgccggctc
    11221 ccgacgatcc gtgcatcctg ccgccgctcg ccggctaccg tggcctcgag aaccgtctgt
    11281 accgggtgga ggtccacgac ggcggcccct taggcaccgc tcgcttcagg tggtcgcgcg
    11341 acaacggctc gatcgtctcg gcggtgaccg acctccaggt gtcgggcggc cagacgacgc
    11401 tgacggtcac ccggatcggg cgcgacccgg tgctccgctt ccgcatcgac gactgggtct
    11461 cggtgaccga cgaccaccgc gagctgtacg aagaaacgcc tgagatggcc caggtcgtcg
    11521 acatcgacga gaccaaccgc cgcatcgtcc tcgaccgcgc cttgcccacc ggcaccgggc
    11581 gcgccttcgc cgccacgccc ggcgagctcg aggaccgccg tacccgcatc cagcgctggg
    11641 accagaccgc ggcgctcaac cccctcgact ccgacggcct gatcacgacg gccgccgggc
    11701 cgatcgacat cgaggagggg attcgcatca gcttcaccgt ggacccggcg ggcggcgcgt
    11761 tccgcaacgg cgactactgg gtgttcgccg cccgcaccgc cgatgcctcg gtcgaggagc
    11821 tggacgaggc gccgccgcgc ggcatccacc atcattacgt ccagcttgcg gctattacgg
    11881 ggctgggcgg cgccaacccg caggtctcgg attgccgtcc gcggcccgag gacggtgttg
    11941 cccagtgctg ctgctgcctg gtcacggtcg gagcgcgaga cgaccagcgc gcggacttct
    12001 tcgacctcgc cggcgccatc gccgccctgc ccaacctggc gccgaacgac tcggtgccgg
    12061 tgatcgtgtg tctgctcgac ggcgaccacg cgctcggcac gccggtggtc gtgacgcgtc
    12121 cgcgcgtgac aattcgcggc tgcggttggg cctcgcgcct gctgcccact caggggccgg
    12181 cgctgtcctt gcgcggcgtc gagcaagcag tcgaggactt ggcgattttc gccgaacaaa
    12241 cgccgccgct gatcgacgcg gccggccagc agctgcggat cgagggctgt cggctggaga
    12301 accaggcctc ggggccggcg gtgctcgccc gccgcgtcct cgacctcaag atcctgcgca
    12361 acctggtgct tgcccagggc ggggggctgg acctggccgg cgacctgatc gaggtcgcgg
    12421 gcaaccgcat cctcggcggg ccggtgcggg tacgcgagcc gtccgacacg gtcgacattc
    12481 gcgacaacga tttgctcggc gcgatcgagg acgcggtcgt cctcggcggc cgcggcatcg
    12541 tctacgagat cgacgtcgcc cacaaccgca tccggggcgc gctgcgccac ggcatcgcca
    12601 gcggcttctt cgaccccgag aaccagggcg gggacggcat cgtcgccggg ctgcgcatcc
    12661 tcggcaacga catcgccgag tgcatcgggc ccaacaccca acgaacggtc aacgacccgc
    12721 ccttcgccgg catcgctcta ggccgcgttt acgacctggt ggcgcgcgac aaccgcatcg
    12781 agcgcaacgg cgagcaggcg accggcccgg tctgcggcat ctacgtccgc cacagccggg
    12841 gagtcgagat cgaccgcaac atcgtgcgca tgaacggccg gcaaccggac ggcacggcga
    12901 tccccggcgt gcaggccggg atcagcctgc gcgatgccac cgtgatgctg tccgagacgc
    12961 ccgacccggc cgccgcgaac cgtagcatcg cggagctcgg catcatgccg gcggcaaaga
    13021 tcaccggcaa cctggtggac tcgcggcgcg ggcccgcgct cagcatcttc gggcaggggc
    13081 cgatgatgat cgagggtaat cgtttccagg ccatcgacgt gctcagcgac ctgtccgacc
    13141 tcaccttctc aaccgtcgat cagtatgtcg gcacggtgtt cgtctacaac tcgggactgc
    13201 cggcctattt cagcggcttc ctgtcgggcg cagggatgaa cgctctgacc acgggcgacg
    13261 tggccgcgct gtcgggctcg ccgctgcaga c
//
LOCUS       DASWHU010000377         8348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1010257, whole genome shotgun sequence.
ACCESSION   DASWHU010000377 DASWHU010000000
VERSION     DASWHU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010257"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGB88_13970"
     CDS             complement(<1..675)
                     /locus_tag="VGB88_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648608.1"
                     /translation="MANSLTVSGLAAGYGAARVLHDVSVTVGDGETVALLGTNGNGKS
                     TLIKCIMGLVRPTAGEIRADIDGRSLDLVGRKPEQVVEAGIVLVPEGRRLFPALTVEE
                     NLMLGAFRRAARAEIASNLALCFEAFPVLRERRTQRAGSMSGGEQQMLALARALMGSP
                     RILLIDEPSVGLAPILVSRVMSKIKELKDQRGLTVLMAEQNFHQATRICDRGYVIVNG
                     RIAFEGK"
     gene            complement(665..1390)
                     /locus_tag="VGB88_13975"
     CDS             complement(665..1390)
                     /locus_tag="VGB88_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648609.1"
                     /translation="MSAAPLLAVAGVTKRFGGFVALDQVSLEVQRGERLGLIGPNGSG
                     KTTLINCISGTLAVDAGRVRFAGADITREAAYRRARRGIARSFQIPRPFRSMTNLDNL
                     MIPLEYAAGAMEGETTHARAHEILDLVGLSGRAGEDCGSLSQVDLRKLELARALAAGP
                     ALLIVDEVMAGLAAAEVDEMLAILAKLNAQGITIVMIEHIMRAVMSFSERIAVLDAGR
                     LIADGKPADVIRDPLVEKAYLGE"
     gene            complement(1387..2331)
                     /locus_tag="VGB88_13980"
     CDS             complement(1387..2331)
                     /locus_tag="VGB88_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648610.1"
                     /translation="MRTRTYVALAAGLVLAALAATRVVGNDYYFFAGYVVLQYVVLAT
                     AWNILGGYTGYVNFGSAAFFAIGAYTTIALIRLAEVPLYLSIPAAGVVCGLIGLAAGY
                     LTLRLRGVYFAIATLALAIVLETFVANWDFVGGATGSIVMRPKEAPLFDSYVKFLFCV
                     MAALAVAAVSVARYLETSRLGRGLAAIRDDEQAAECSGVPTLRLKLLSATLSGALMGL
                     AGAPFPYYVTYVDPPSAFNLDYAVNAVAMPMVGGTQTWLGPVVGALLLGTLQQVATVT
                     ISSELNLLIVGLVLVGFVILAPEGIVGQVRRVLARRRR"
     gene            complement(2335..3201)
                     /locus_tag="VGB88_13985"
     CDS             complement(2335..3201)
                     /locus_tag="VGB88_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648611.1"
                     /translation="MSDLFLSALASGLMLGGFYAAVTLGLTLAFGYLNVVNIAHPAFV
                     LLGSYAVYGVNARWGVDPVLAGAGLAPLFFVLGVAVYSLYQASFEAREDESLRGLAFF
                     FGLMFLIEVGLILSYGVDYRLVSAPYIGPTVALGAVLLPTRMLVPFLVALAMVWALQL
                     YLARTFTGRAIRAVAQDQQALRLVAADPVRIKRLAFGIAIATACIAGALLVVIGPVEP
                     SMGRAYIGRVFAIVVLGGMGSAGGTLLAALVLGLAESITSTYYGPSWSPAVAFGFLLF
                     ALAVRPAGLLGR"
     gene            complement(3234..4511)
                     /locus_tag="VGB88_13990"
     CDS             complement(3234..4511)
                     /locus_tag="VGB88_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9648612.1"
                     /translation="MPKLVSNRRRSGRWARAALGAVMLMLGAAVPAGAAEPIRIGFGM
                     ALTGALAGAGKAALIAIQIWAEDVNAAGGLLGRKVELVYYDDQTNASKVPGIYTKLIN
                     VDRVDLIVSGYGSAVIAPAIPIAMNYDMVFMSLFGLATNDDFGYDRYFQILPAGPEPR
                     IAWSKGFFDLALSLDPKPRTVALVGADAEFPRNAMIGARVNAERAGLKVVYDRSYPPT
                     TVDFTPVIRSIQAENPDVVYVASYPPDSAGIVRAVSELGLNAALFGGGMVGLQYASFE
                     EALGPLLNGIVNYDFWVPEPTLKFPGIEAFLAKYQAVAADAGVDPLGYYLPPFAYANM
                     QILGDAVEAVGKIDQDALADYIRSHEFETVVGNIRFGPGGEWQKGRILMVQFQGLKDN
                     SLEQFTRPGTRVVLLPEDWRSGSLITPFAKARP"
     gene            complement(4592..5695)
                     /locus_tag="VGB88_13995"
     CDS             complement(4592..5695)
                     /locus_tag="VGB88_13995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9648613.1"
                     /translation="MKLGAFMMPLHPPARPYLETLREDQQAIVLADKLGYCEAFVGEH
                     VTDRAESVTSSLLFIASVAGEARTITLGSGTVNLPNHHPLRVAAEVAMLDTLLEGRFL
                     FGISPGGLLTDAEAFGNLDADRTAMFVEAIDQILAIWAGEPPYDLDGAFWRISTARTM
                     DLAIGQGLVAKPYQKPHPPIVVTAVAPFSKGVVEAAARGWTPISANFLQPEWVASHWP
                     KYREGCALGGRVARPADWRVAKSIFVADDQATARAYAKSADGPYGFYYAQLMRKLIGN
                     ARGNLFKADPDMADDALSLDYVLDSLVICGTVEHVVDQLLAFRDRIGDFGTLLYALHD
                     WADARLARRSMELMADAVMPALNKAIGVEVAAE"
     gene            complement(5712..6947)
                     /locus_tag="VGB88_14000"
     CDS             complement(5712..6947)
                     /locus_tag="VGB88_14000"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9648614.1"
                     /translation="MGDAATGGGRSALIIGGSVAGLFAASLLARAGWRVQVFERSADE
                     LASRGAGIATHPELMTALRRAGVEVDDGFGVEIAGRITFALDGSEIGRHDMPQIMTSW
                     DLLYRRLFACLPAGAYHGGVPLERIEQRPGRVIAHFAGGGSAEGDLLVGADGVRSTVR
                     SQYLPRVNPEYAGYVAWRGMVPEPLVSEATRQVLDDRLSFCLPPGEQFLHYPIANEDG
                     VLTRGRRRMNWVWYRHARPGEELELLLTDRDGRRHDVSMPPDKIGPACVAAMRADAAR
                     GLPAQYAELVRLTERPFLQAIFDVDSPEIRFGRAVLVGDAAFVARPHTGFGVTKAALD
                     VVALADALSTDADIDRALERWHQARLAFGKAAVVRGRVLGRMLDADRSAPAGDSFGLG
                     RGVPRAVMAETGISDAMQP"
     gene            7042..8310
                     /locus_tag="VGB88_14005"
     CDS             7042..8310
                     /locus_tag="VGB88_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9648615.1"
                     /translation="MYRPRMTLAFDPDRPEVLADPYPLYRRLRAEDPVHDSDALGGWV
                     LTRYDDVKLALRDARFSADRATPFAEHMERDARPELARMGRILSQWVVFNDPPRHAVL
                     RRLMVDAFTPRAAESLRARIAEIVDDLIGRLKGRARLDLIADFAYPLPATVIAHILGV
                     PDRDLDRFRAWSDDLVAVIASMRTLPDRMARGALALAEVSDYLAGIVGARKRSGARGA
                     LVDDLIAAHEADDALSFDELVANCVLILFAGHETTTNLIGNGMRLLLERPDQLARLSA
                     DRALMAGAVEEMLRFESPAHSVARVAAEDVELAGTTIRRGERVFAMIGAANRDPDLVD
                     DPERFDVTRPPSRHLSFGYGPHFCVGAPLARIEGEIALARLLDAFPALALEPGPLDWE
                     PSLVLRGLKTLPLAVAGTADRQEAKALASQ"
BASE COUNT         1272 a         2980 c         2957 g         1139 t
ORIGIN      
        1 cttgccctcg aaggcgatgc ggccgttgac gatgacgtag ccgcggtcgc agatgcgggt
       61 cgcctggtgg aagttctgct cggccatcag cacggtgagc ccgcgctggt ccttgagctc
      121 cttgatcttg ctcatcaccc ggctgaccag gatcggcgcg aggccgaccg agggctcgtc
      181 gatcaagagg atgcgcggcg agcccatcag cgcgcgcgcg agtgccaaca tctgctgctc
      241 gccgccgctc atgctgcccg cgcgctgggt gcgccgctcc ctgagcaccg ggaacgcctc
      301 gaagcagagc gcgagattgc tcgcgatctc ggcgcgcgcc gcgcgccgga acgcgcccag
      361 catcaggttc tcctcgacgg tgagcgcggg gaacaggcgc cggccctcgg gcaccagcac
      421 gatgccggcc tcgaccacct gctcgggctt acgcccgacc aggtcgaggc tgcggccgtc
      481 gatgtcggcg cgaatctcgc ccgccgtcgg gcgcaccagc cccatgatgc acttgatcag
      541 cgtgctcttg ccgttgccgt tagtgcccaa cagcgccacc gtctcgccgt cgccgacggt
      601 gaccgagacg tcgtggagca cgcgcgccgc gccgtagccc gcggcaaggc cgctgacggt
      661 gaggctattc gccaagatac gccttctcca cgagcgggtc gcggatcacg tccgcgggct
      721 tgccgtcggc gatcagccgg ccggcgtcga gcacggcgat gcgctcggag aagctcatca
      781 ccgcgcgcat gatgtgctcg atcatgacga tggtgatgcc ttgcgcgttc agcttggcca
      841 ggatcgccag catctcgtcg acctcggcgg cggcgaggcc ggccatcacc tcgtcgacga
      901 tcaggagcgc aggtcccgcg gcgagcgcgc gcgcgagctc gagcttgcgc aggtcgacct
      961 gcgacaggct gccgcagtcc tcgcccgcgc gcccgctgag cccgaccagg tcgaggatct
     1021 cgtgggcccg cgcatgcgtg gtctcgccct ccatcgcgcc ggcggcgtat tcgagcggga
     1081 tcatcaggtt gtcgagattg gtcatcgagc ggaacgggcg cgggatctgg aagctgcgcg
     1141 cgatgccgcg gcgcgcgcgc cgataggcgg cctcgcgggt gatgtcggcg ccggcgaagc
     1201 gcacccgccc ggcatcgacg gcgagcgtgc cgctgatgca gttgatcagc gtggtcttgc
     1261 ccgagccgtt gggcccgatc aggccgagcc gctcgccgcg ctggacctcg agcgacacct
     1321 ggtcgagggc gacgaagccg ccgaagcgct tggtcacgcc cgcgaccgcg agcagcggcg
     1381 ccgcgctcac cgccgcctcc gcgccagcac gcggcgcacc tggccgacga tcccctcggg
     1441 cgccaggatc acgaagccga ccagcaccag gccgacgatc aaaaggttga gctcggacga
     1501 gatggtcacc gtcgccacct gctgaagcgt gcccaagagc agcgcgccga ccaccggccc
     1561 gagccaggtc tgggtgccgc cgaccatcgg catggcgacg gcattgaccg cgtagtcgag
     1621 gttgaacgcc gagggcgggt cgacataggt gacgtaatag gggaacggcg cgccggcgag
     1681 gcccatcagc gcgccgctta acgtcgccga cagcagcttg agcctgagcg tgggcacgcc
     1741 cgagcactcg gccgcctgct cgtcgtcgcg gatcgcggcg agcccgcgcc cgagcctgga
     1801 ggtctcgagg tagcgcgcca ccgacaccgc ggccacggcc agcgccgcca tcacgcagaa
     1861 caggaacttc acatagctgt cgaacagcgg cgcctccttc ggtcgcatga cgatcgagcc
     1921 ggtggcgccg ccgacgaagt cccagttggc gacgaaggtc tcgagcacga tggcgagcgc
     1981 gagcgtggca atggcgaaat agaccccgcg caggcgaagc gtgagatagc cggcggcgag
     2041 cccgatcagc ccgcacacca cgccggccgc cgggatcgac agataaagcg gcacctcggc
     2101 cagcctgatc agcgcgatcg tggtgtaggc gccgatggcg aagaatgccg cgctaccgaa
     2161 gttgacgtag ccggtgtagc cgccgaggat gttccacgcg gtcgccagca cgacgtactg
     2221 gagcaccacg tagccggcga agaagtagta gtcgttgccg accacgcggg tcgcggcgag
     2281 cgcggccagc accaggccgg cggcaagcgc gacataggtg cgggtgcgca cgccttacct
     2341 gcccagcagc ccggcggggc gcacggcgag cgcgaacaac aggaacccga acgccaccgc
     2401 cggcgaccac gacggcccgt aataggtcga ggtgatgctt tcggcgaggc cgaggaccag
     2461 cgccgcgagc aaggtgccgc cggcgctgcc catgccgccg agcacgacga tcgcgaacac
     2521 gcgcccgata taggcccggc ccatcgacgg ctcgaccggg ccgatcacca ccagcagcgc
     2581 gccggcgatg caggcggtgg cgatggcgat gccgaaggcc aggcgcttga tccgtaccgg
     2641 gtcggcggcg accagtctca gcgcctgctg gtcctgcgcc accgccctga tcgcgcgccc
     2701 ggtgaaggtg cgcgcgagat acagctgcag cgcccacacc atcgccagcg cgaccaggaa
     2761 cggcaccagc atccgggtcg gcagcagcac cgcgccgagc gcgacggtgg gaccgatata
     2821 gggtgctgac accagccggt agtcgacgcc gtagctgagg atcaggccga cctcgatcag
     2881 gaacatcagc ccgaagaaaa acgcgagccc gcgcaaggac tcgtcctcgc gcgcctcgaa
     2941 gctcgcctgg tagagcgagt acaccgcgac cccgagcaca aagaacagcg gcgccaggcc
     3001 cgcgcccgcc agcacggggt cgacacccca gcgtgcattg actccgtaca ccgcgtagga
     3061 gcccagcagc acgaacgccg gatgggcgat gttgaccacg ttcaggtagc cgaacgcgag
     3121 cgtcagcccg agcgtgaccg cggcgtagaa gccgccgagc atcagcccgg aggcgagcgc
     3181 gctcaggaac aggtcgctca agacacttgc cgcgcgcccg ccctggcggg cggctagggc
     3241 ctcgcctttg cgaacggcgt gatcaggctg cccgagcgcc agtcctcggg cagcagcacc
     3301 acccgcgtgc ccggcctggt gaactgctcg aggctgttgt ccttgaggcc ctggaactgg
     3361 accatcagga tgcgcccctt ctgccattcg ccgccgggtc cgaagcggat gttgccgacc
     3421 acggtctcga actcgtggct gcggatgtag tcggcgagcg cgtcctggtc gatcttgccc
     3481 accgcctcga ccgcgtcgcc gagaatctgc atgttggcgt aggcgaacgg cggcaggtaa
     3541 tagccgagcg ggtcgactcc ggcgtcggcg gcgaccgcct ggtacttggc gaggaacgcc
     3601 tcgatgcccg ggaacttgag cgtcggctcg ggcacccaga agtcataatt gacgatgccg
     3661 ttgagcagcg gcccgagcgc ctcctcgaac gaggcgtact ggaggccgac catgccgccg
     3721 ccgaacaggg cggcgttcag gccgagctcg ctcaccgcgc gcacgatgcc ggccgaatcc
     3781 ggcgggtagg aggcgacgta gacgacatcc ggattctccg cctggatgga gcggatcacc
     3841 ggcgtgaagt cgaccgtggt cggcgggtag ctgcggtcgt agacgacctt gagcccggcg
     3901 cgctcggcat tgacgcgggc gccgatcatg gcgttgcgcg ggaactcggc atcggcgccg
     3961 accagggcca cggtccgggg cttcgggtcg agcgagaggg cgagatcgaa gaagcccttc
     4021 gaccaggcga tgcgcggctc cggccccgcc ggcaggatct ggaagtagcg gtcgtagccg
     4081 aagtcgtcgt tggtggccag cccgaacagc gacatgaaca ccatgtcgta gttcatcgcg
     4141 atcgggatcg ccggcgcgat caccgccgag ccgtagccgg acacgatcag gtcgacccgg
     4201 tcgacgttga tcagcttggt gtagatgccc ggcaccttgg aggcgttggt ctggtcgtcg
     4261 tagtagacca gctcgacctt gcgcccgagc aggccgccgg cggcattgac gtcctcggcc
     4321 cagatctgga tcgcgatcag cgccgccttg ccggcgccgg cgagcgcgcc ggtgagcgcc
     4381 atgccgaagc cgatgcggat cggctcggcc gcgcccgccg ggacggcggc gccgagcatc
     4441 agcatcaccg cgccgagcgc ggcccgcgcc caccggccgc tgcgccgccg gttgcttacg
     4501 agctttggca tgacgtctcc tcccctggtt cgttgcgctg tcgcccgcgg ccggctcccc
     4561 tctcgccggc acgggcattc cagcagattc cctactcggc ggccacctcg acgccgatcg
     4621 ccttgttgag cgcgggcatc accgcgtccg ccatcagctc catcgaccgg cgcgcgagcc
     4681 gcgcgtcggc ccagtcgtgg agcgcgtaca gcagggtgcc gaagtcgccg atccggtcgc
     4741 ggaacgcgag cagctggtcg accacgtgct cgacggtgcc gcagatgacg agggagtcga
     4801 gcacgtagtc caagctcagc gcgtcgtccg ccatgtcggg gtcggccttg aacaggttgc
     4861 cgcgcgcgtt cccgatcagc ttgcgcatca gctgcgcgta gtagaagccg taggggccgt
     4921 ccgccgactt ggcgtaggcg cgcgcggtcg cctggtcgtc ggcgacgaag atgcttttcg
     4981 ccacccgcca gtcggccggc ctggcgacgc gcccgccgag cgcgcagccc tcccggtatt
     5041 tgggccagtg gctcgccacc cactcgggct gcaggaagtt ggccgagatc ggcgtccagc
     5101 cgcgcgccgc cgcctcgacc acccccttcg agaacggcgc cactgcggtg accacgattg
     5161 gcgggtgcgg cttctgatag ggcttggcca ccaagccctg gccgatcgcc aggtccatgg
     5221 tgcgcgcggt cgagatgcgc cagaacgcgc cgtcgagatc gtagggcggc tcgcccgccc
     5281 agatcgcgag gatctggtcg atcgcctcga cgaacatggc ggtgcggtcg gcatcgagat
     5341 tgccgaacgc ctccgcgtcg gtgagcaggc cgcccgggct gatgccgaac aggaagcggc
     5401 cctcgagcag ggtatcgagc atcgccacct ccgccgcgac tctcagcggg tggtggttgg
     5461 gcaggttgac cgtgccgctg ccgagggtga tggtgcgcgc ctcgcccgcg acgctggcga
     5521 tgaacagcag gctcgaggtg accgactcgg cgcggtcggt gacgtgctcg ccgacgaacg
     5581 cctcgcagta gccgagcttg tcggcgagca cgatcgcctg ctggtcctcg cgcaaggtct
     5641 ccagataggg ccgcgcgggc gggtgcagcg gcatcatgaa cgcgccgagc ttcatccccg
     5701 tctcgcccgc ctcacggctg catcgcgtcc gagatgccgg tttcggccat caccgcgcgc
     5761 ggcacgccgc gcccgaggcc gaacgagtcc ccggccggcg cgctgcggtc ggcatcgagc
     5821 atccgcccca acacgcgccc gcgcacgacc gccgccttgc cgaaggcgag gcgcgcctgg
     5881 tgccagcgct cgagcgcgcg gtcgatgtcg gcgtccgtgc tgagcgcatc cgcgagcgcg
     5941 accacgtcga gcgccgcctt ggtcaccccg aagccggtat gcgggcgggc gacgaaggcg
     6001 gcgtcgccca ccagcaccgc gcgcccgaac cggatctcgg gactgtcgac gtcgaagatc
     6061 gcctgcagga acggccgctc ggtgagccgc accagctcgg catattgcgc gggcaggccg
     6121 cgcgcggcat cggcgcgcat cgcggcgacg caggccgggc cgatcttgtc cggcggcatc
     6181 gacacgtcgt ggcgccggcc gtcgcggtcg gtgagcaaca gctcgagctc ctcgccgggc
     6241 cgggcgtggc ggtaccacac ccagttcatg cgccgccggc cgcgggtcag cacgccgtcc
     6301 tcgttggcga tcgggtaatg caggaactgc tcgccgggcg gcaggcagaa cgacagccgg
     6361 tcgtccagca cctgccgggt ggcctcggac accagcggct cgggaaccat gccgcgccag
     6421 gcgacgtagc cggcatattc ggggttgacg cggggcagat actggctgcg cacggtcgag
     6481 cgcacgccgt ccgcgccgac cagcaggtcg ccctcggcgc tgcccccgcc agcgaagtgc
     6541 gcgatcaccc ggcccgggcg ctgctcgatg cgctcgagcg gcacgccgcc atgataggcg
     6601 ccggccggca ggcacgcgaa caggcgccgg tacagcaggt cccacgaggt catgatctgg
     6661 ggcatgtcgt ggcggccgat ctccgagccg tcgagggcga aggtgatgcg gccggcgatc
     6721 tcgaccccga agccgtcgtc gacctcgacg ccggcgcgcc tgagcgcggt catcagctcg
     6781 ggatgggtgg cgatgccggc gccgcggctg gcgagctcgt ccgcggagcg ctcgaacacc
     6841 tggacccgcc agcccgcgcg ggcgagcagc gaggcggcga acaggcccgc gaccgagccg
     6901 ccgatgatca gggcgcttcg cccgccgccc gtggccgcgt cgcccatggg tctcccgcgt
     6961 ggtcgtggta cctgcgcgcg gccattatgg cacgcccgct gacgccgggg ctacgcccga
     7021 ctgggcgttt ggcttggcgt cgtgtatcgt ccgcgcatga cgctcgcctt cgaccccgac
     7081 cgtcccgagg tgctggcgga cccgtatccg ctgtaccggc ggctgcgcgc cgaggacccg
     7141 gtccatgaca gcgacgcgct cggcggctgg gtgctgaccc gctacgacga cgtcaagctg
     7201 gcgctgcgcg atgcgcgctt ctcggccgac cgggcgacgc cgttcgccga gcacatggag
     7261 cgggacgcgc gccccgagct cgcgcgcatg ggccgcatcc tgagccagtg ggtggtgttc
     7321 aacgacccgc cgaggcatgc ggtgctgcgc cggctgatgg tcgatgcgtt caccccgcgc
     7381 gcggccgaaa gcttgcgcgc gcgcatagcc gagatcgtcg acgacctgat cgggcggcta
     7441 aaggggcgcg cgcggctcga cctgatcgcc gacttcgcct atccgctgcc cgcgaccgtg
     7501 atcgcccaca tcctgggcgt gcccgaccgc gacctcgacc gcttccgcgc ctggtcggac
     7561 gacctggtgg cggtgatcgc ctcgatgcgc acgctgcccg accgcatggc gcgcggcgcg
     7621 ctggccctcg ccgaggtgag cgactacctc gccggcatcg tcggtgcgcg caagcgaagc
     7681 ggcgcgcgcg gcgcgctggt cgatgacctg atcgccgcgc acgaggcgga tgacgcgctg
     7741 agcttcgacg agctggtggc gaactgcgtg ctgatcctgt tcgccggcca cgagaccacc
     7801 accaacctga tcggcaacgg catgcggctg ctgcttgagc ggcccgacca gctcgcccgc
     7861 ctcagcgccg atcgcgcgct catggcgggc gcggtggagg agatgctgcg cttcgaatcg
     7921 ccggcgcatt cggtggcgcg ggtcgccgcc gaggacgtgg agctcgccgg caccacgatc
     7981 cggcgcggcg agcgcgtctt cgccatgatc ggcgccgcca accgcgaccc ggacctggtc
     8041 gacgatcccg agcgcttcga cgtgacccgg ccgccgtcgc ggcacctgtc gttcggctac
     8101 ggcccgcatt tctgcgtcgg cgcgccgctc gcacgaatcg aaggcgagat cgcgctcgcc
     8161 cgcctgctcg acgcgttccc ggcgctcgcg ctcgagccgg ggccgctcga ctgggagccg
     8221 tccttggtgc tccgcggcct caagaccctg ccgctcgccg tcgccggcac ggccgaccgt
     8281 caagaggcca aggcgctcgc gtcgcagtga gaccggtcat tcgccgacgg ttgcctcgct
     8341 aacgatag
//
LOCUS       DASWHU010000378         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1011625, whole genome shotgun sequence.
ACCESSION   DASWHU010000378 DASWHU010000000
VERSION     DASWHU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011625"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VGB88_14010"
     CDS             <1..930
                     /locus_tag="VGB88_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9648616.1"
                     /translation="LKLGAGDLCLNVMPLFHIHGLVAGVLSSLAAGAAVACTPGFNAL
                     RFYAWLDELKPTWYSAVPTMHQAILARAGRNADAIARSRLRLIRSSSASLPPQVMAAL
                     EETFGAPVIESYGMTEASHQMTSNPLPPAARKPGTVGIAAGPEVRVLAAPGAAGEVVI
                     RGPNVTRGYENNPKANAEGFTDGWFRTGDEGRLDAEGYLTITGRLKEIINRGGEKVSP
                     KEVDEVLMDHPAVAQVVTFAMPHPKLGEEVAAAVVLREGASASERDIRDFAARRLADF
                     KVPRKVVFLDEIPKGATGKLQRIGLAERLGVSA"
     gene            938..1921
                     /locus_tag="VGB88_14015"
     CDS             938..1921
                     /locus_tag="VGB88_14015"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9648617.1"
                     /translation="MKICVYGAGAIGGYVAARLAHAGAEVTVIARGDKLAAIRTHGLK
                     LIQERGDIVTRPAATDDPAEAGPQDYVIVAMKAHAVPAVAARMAPLLGPATAVVPAVN
                     GVPWWYFYRLDGPYRDRRLETVDPGGAQWRHIGPERVIGCVVYPAAEVAAPGVVRHIA
                     LDRMPIGEPDGSRSERALRLSEMMILAGMKAPVRARIRDDIWVKLWGNVSFNPVSVLT
                     GATLGEIARDAGTRAVVRAMMVEAQAVGETLGVRFPIGVDARIEGGAEVGDHKTSTLQ
                     DLEAGRPLELAPLVGAVAELGDVVGIDTPTIDMIYALARQRAITAGCWPEG"
     gene            complement(1953..2609)
                     /locus_tag="VGB88_14020"
     CDS             complement(1953..2609)
                     /locus_tag="VGB88_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9648618.1"
                     /translation="MAKTRGLPAVLVSALVVIAAAQVARADELAGAALVEALRAGGYT
                     IYVRHAATDWGQHDRVAAAGDWTSCEPSEMRQLSAEGREAARRLGAAIRALAIPVAKV
                     LSSEYCRAVETARLMDLGPVATTREIMNTRAAEYVGGLEAAIRRARRVLATRPAEGAN
                     TVIVGHGNLMRAATGAYPEEGGSGVFAADPDAPHGFVLVALLSADDWLRLAERFARDG
                     "
     gene            complement(2638..>2926)
                     /locus_tag="VGB88_14025"
     CDS             complement(2638..>2926)
                     /locus_tag="VGB88_14025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9648619.1"
                     /translation="PPLAKRLPHYPVPAAVLGRLAVDLSYQRRGLGELLLLDAVRRVL
                     RASVALAVYAVVVDAKDERAETFYRRYGFVPFASLPRRLFLPLETFVKAGL"
BASE COUNT          417 a         1051 c         1080 g          378 t
ORIGIN      
        1 ctcaagctcg gggcgggcga cctgtgcctc aacgtcatgc cgctgttcca catccacggg
       61 ctggtcgcag gcgtgctgtc ctcgcttgcc gccggggctg cggtggcgtg cacgccgggg
      121 ttcaacgcgc tccgcttcta cgcctggctc gatgagctca agccgacctg gtacagcgcg
      181 gtgccgacca tgcaccaggc gatcctcgcc cgcgccgggc gcaacgcgga cgcgatcgcg
      241 cgctcgcgcc tgcggctgat ccgctcgtcg tcggcctcgc tgccgccgca ggtgatggcg
      301 gcgctggagg agacgttcgg cgcgccggtg atcgagtcct acggcatgac cgaggcgagc
      361 caccagatga cctcgaaccc gttgccgccg gcagcgcgca agccgggcac ggtcggcatc
      421 gccgccggcc ccgaggtgcg cgtgctggcg gccccgggcg cggcgggcga ggtggtgatc
      481 cgcggcccca acgtcacccg cggctacgag aacaacccca aggccaacgc cgagggcttc
      541 accgacggct ggttccgcac cggcgacgag ggccgcctcg atgccgaggg ctacctgacc
      601 atcaccggcc ggctcaagga gatcatcaac cgcggcggcg agaaggtcag ccccaaggag
      661 gtcgacgagg tgctgatgga ccacccggcg gtggcccagg tggtgacctt cgccatgccg
      721 cacccgaagc tcggcgagga ggtggcggcg gcggtggtgc tgcgcgaggg cgcgagcgcg
      781 agcgagcgcg acatccgcga cttcgccgcc cgccgcctcg ccgacttcaa ggtgccgcgc
      841 aaggtggtgt tcctcgacga gatcccgaag ggcgcgaccg gcaagctgca gcgcatcggc
      901 ctcgccgagc ggctcggggt gtccgcctga gcgcgacatg aagatctgcg tctacggcgc
      961 cggcgcgatc ggcggctacg tcgccgcgcg gctggcgcac gcgggcgccg aggtgacggt
     1021 gatcgcgcgc ggcgacaagc tcgccgcgat ccgcacgcac gggctcaagc tgatccagga
     1081 gcggggcgac atcgtcaccc ggccggccgc gaccgacgac cctgccgaag ccggcccgca
     1141 ggactatgtc atcgtcgcca tgaaggcgca cgcggtgccg gcggtggcgg cgcgcatggc
     1201 gccgctgctc ggcccggcga ccgccgtggt gccggcggtc aacggcgtgc cgtggtggta
     1261 cttctaccgg ctcgacgggc cgtaccggga ccgccggctc gagaccgtcg acccgggcgg
     1321 cgcgcagtgg cgccacatcg ggcctgagcg ggtgatcggc tgcgtcgtct atccggcggc
     1381 cgaggtggcg gcgccgggcg tggtgcgcca catcgcgctt gaccggatgc cgatcggcga
     1441 gcccgacggg tcgcgctcgg agcgcgcgct gcggctcagc gagatgatga tcctggccgg
     1501 catgaaggcg ccggtgcgcg cgcgcatccg cgacgacatc tgggtcaagc tgtggggcaa
     1561 cgtgtcgttc aacccggtga gcgtgctgac cggcgcgacc ttgggcgaga tcgcgcgcga
     1621 tgccggcacg cgtgcggtgg tgcgggcgat gatggtcgag gcgcaggcgg tcggcgagac
     1681 gctcggcgtg cgcttcccga tcggcgtcga tgcgcgcatc gagggcggcg ccgaggtcgg
     1741 cgaccacaag acgtccaccc ttcaggacct cgaggccggc cgccccctcg agctcgcgcc
     1801 gctcgtcggc gcggtcgccg agctcggcga cgtggtcggc atcgacacgc ccaccatcga
     1861 catgatctac gccctcgccc gccagcgcgc gatcaccgcc ggctgctggc cggaggggtg
     1921 accagctgcg ccgggcgaaa gggaagccgg tttcagccgt ctctggcgaa gcgctcggcg
     1981 aggcgcagcc agtcgtcggc ggacaggagc gcgaccagca cgaagccgtg cggggcgtcc
     2041 gggtcggcgg cgaacacgcc gctgccgcct tcctcgggat aggcgccggt cgcggcgcgc
     2101 atgaggttgc cgtggccgac gatgacggtg ttcgcgcctt ccgccggccg cgtcgccagg
     2161 acccgccgtg cgcgccggat cgccgcttcg agtccgccga cgtattcggc ggcgcgcgtg
     2221 ttcatgatct cgcgcgtggt cgcgaccggc ccgaggtcca tcaggcgcgc ggtctccacc
     2281 gcccggcagt actcgctcga cagcaccttg gcgacgggga tggcgagtgc gcggatcgcg
     2341 gcgccgagcc gccgcgccgc ctcgcgcccc tcggccgaga gctggcgcat ctccgaaggc
     2401 tcgcagctgg tccagtcacc ggccgccgcg acccggtcgt gctggcccca gtcggtcgcc
     2461 gcgtggcgga cgtagatggt gtagccgccg gcgcgcagcg cctcgaccaa tgccgcgccg
     2521 gcaagctcgt ccgcgcgcgc aacttgagcc gcggcgatga cgacgagcgc cgagaccagc
     2581 accgccggca gtccacgagt cttcgccatg acgcgccgcc gcaccgtttc ccacagtcta
     2641 caggcctgcc ttgacgaaag tctccagtgg caagaacagc cggcgcggca atgatgcgaa
     2701 ggggacgaag ccatagcgcc gatagaatgt ttccgcgcgt tcgtccttgg catccaccac
     2761 caccgcatat acggcgaggg cgacacttgc gcggagcacg cgccgaacag cgtcgagcag
     2821 gagcaactcg cccaagcccc ttcgctggta cgaaagatcg accgcgagcc ggccgagcac
     2881 cgccgccggc accggatagt gcggcaaccg cttggccaag ggcggc
//
LOCUS       DASWHU010000379         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_634483, whole genome shotgun sequence.
ACCESSION   DASWHU010000379 DASWHU010000000
VERSION     DASWHU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634483"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VGB88_14030"
     CDS             complement(<1..714)
                     /locus_tag="VGB88_14030"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390194.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX9648620.1"
                     /translation="MRIERLFTRAGESPYHTIPFRPAASEIRNPDGSVVFRRDDIEVP
                     AAWSQVAVDILAQKYVRKAGVAATLKPVAEADVPEWLWRRRPDPDSPAEPVAKAAATG
                     GETKTVQVFDRMAGAWTYWGWKGGYFDTEADARAFYDELRYMLARQVAAPNSPQWFNT
                     GLHWAYGIDGPAQGHYYVDHRSGALKRSESAYERPQPHACFIQGVADDLVNEGGIMDL
                     WVREARLFKYGSGTGSNFSA"
     gene            complement(978..>1852)
                     /locus_tag="VGB88_14035"
     CDS             complement(978..>1852)
                     /locus_tag="VGB88_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9648621.1"
                     /translation="EAFRALRDEPSQSAIAARWEAFLALALKDPPPYHLRLALREIRR
                     YQGARAADSITVLDHGCGDGLSVLYLRALGYRACGVDLRESAHFARINAELGSEVLQG
                     YDGRRLPFPDASVDVVLSQQVLEHVADNLVEAYYAEEGRVLKPGGIALHQVPHRLVPF
                     DSHTQTWFTHWLPAPLRNALYRRLGFKSLPQRLHYRTPWFHRAAARRHIGLCDDLTIE
                     RLRLVRDFDAGTGPVYDGNVGLRRLVDRAIHAPAVGQLLGRMVRPFVMLDSVSHKRPA
                     QPRQDLAGGGFGPG"
BASE COUNT          271 a          627 c          676 g          278 t
ORIGIN      
        1 cgccgagaag ttggagccgg tgcccgagcc gtacttgaac aggcgcgcct cgcgcaccca
       61 caggtccatg atgccgccct cgttcaccag gtcgtcggcg acgccctgga tgaagcaggc
      121 gtgcggctga ggccgctcgt acgcggattc cgagcgcttc agcgcgccgc tccggtggtc
      181 gacgtagtag tggccctggg ccgggccgtc gatgccatag gcccagtgca gcccggtgtt
      241 gaaccactgc gggctgttgg gcgccgcgac ctggcgggcg agcatgtagc ggagctcgtc
      301 gtagaacgcg cgcgcatccg cctcggtgtc gaagtagccg cccttccagc cccaataggt
      361 ccacgccccg gccatgcggt cgaacacctg caccgtcttg gtctcgccgc ccgtggcggc
      421 tgccttggcg actggctcgg cgggcgaatc ggggtcggga cggcggcgcc acagccactc
      481 gggcacgtcg gcctcggcga ccggcttgag cgtcgcggcc acgcccgcct tgcggacgta
      541 cttctgggcc aggatgtcga ccgcgacctg cgaccaggcg gcgggcacct cgatgtcgtc
      601 gcggcggaac accaccgagc cgtcggggtt gcgaatctcg ctggcggcgg gccggaatgg
      661 gatggtgtgg tatggcgact cgcccgcccg cgtgaagagt cgctcgattc gcatcggtgc
      721 ccccggctgg ccgcccgcgc actgttgtcg gcgcgattca gctttatgtt gcgcggcccc
      781 atatctggtg tatgggtgct cgcctcgcct caaactctag tgccggtcac agggccgtca
      841 agagcgaatc accacatagg acgcgctcgc ggcatatccg cctcaacatg ttgcaggcag
      901 atgacatgcg gacggaaata tcgcgatctc agccctttgc cgccgattgc tcgcgccggc
      961 gcgaagccgg cgccggctta gcccgggccg aagccgccgc cggcaaggtc ctggcggggt
     1021 tgcgccgggc gcttgtggct gaccgagtcg agcatcacga acgggcgcac catccggccg
     1081 aggagctgcc cgaccgcggg cgcgtggatg gcgcggtcga ccaggcgcct gaggccgaca
     1141 ttgccgtcat agaccgggcc ggtgccggcg tcgaagtcgc ggaccagcct gagccgttcg
     1201 atggtcaggt cgtcgcaaag cccgatatgg cggcgcgcgg cggcgcggtg gaaccacggc
     1261 gtgcggtaat gcaggcgctg gggcagcgac ttgaagccga gccggcggta gagcgcgttg
     1321 cgcagcggcg ccggcagcca atgggtgaac caggtctggg tgtgcgaatc gaacggcacc
     1381 agtcggtgcg gcacctggtg cagcgcgatg ccgccgggct tgagcacccg gccttcctcg
     1441 gcgtagtacg cctcgaccag gttgtcggcg acgtgctcga gcacctgctg gctgaggacg
     1501 acgtcgacgc tggcgtcggg gaatggcagg cgccggccgt catagccctg cagcacctcc
     1561 gagccgagct cggcattgat gcgtgcgaag tgcgcgctct cccgcaggtc gacgccgcag
     1621 gcgcgatagc ccaacgcgcg caggtaaagg acgctcagcc cgtcgccgca gccgtggtcg
     1681 agcacggtga tcgaatccgc cgcgcgcgcg ccctgataac ggcggatctc gcgcagcgcg
     1741 agccgcaggt ggtagggcgg cgggtccttg agcgcgaggg cgaggaacgc ctcccagcgc
     1801 gccgcgatcg cgctttgcga cggctcgtcg cgcagcgcgc ggaacgcctc ct
//
LOCUS       DASWHU010000380        23208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2134765, whole genome shotgun sequence.
ACCESSION   DASWHU010000380 DASWHU010000000
VERSION     DASWHU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 23208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23208
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134765"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGB88_14040"
     CDS             complement(<1..185)
                     /locus_tag="VGB88_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEX9648622.1"
                     /translation="MVGGHHVKAGGRAVIELKIAELSTHTPIGLPVQVLHGRRDGPRL
                     LVCAALHGDEINGVEIIR"
     gene            complement(205..1110)
                     /gene="rimK"
                     /locus_tag="VGB88_14045"
     CDS             complement(205..1110)
                     /gene="rimK"
                     /locus_tag="VGB88_14045"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007206460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX9648623.1"
                     /translation="MKIAMLARNPGLYSHQRLIAAARQRGHDIEVYNTLMCYMNITAH
                     RPEVRYGGEVLSGIDAVIPRIGASITFYGLAVLRQFEMMGVWPLNESVAIGRSRDKLR
                     SLQLLARQGIGLPVTGFAHATRYTDDLIEMAGGAPVVVKLLEGTQGLGVVLGETHNSA
                     KSVIEAFRGAKVNILVQEYIKEAGGADIRAFVIGDRVAAAMQRKAPEGEFRSNIHRGG
                     SAARIRITPEERSTAVRAARAMGLNVAGVDMLRSNHGPVVMEVNSSPGLEGIEAATEV
                     DIAGKVIEFVEKHARPHRTRTRGKG"
     gene            complement(1107..1607)
                     /locus_tag="VGB88_14050"
     CDS             complement(1107..1607)
                     /locus_tag="VGB88_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010386450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX9648624.1"
                     /translation="MDDEHRHAVGWREWVALPALGVEKVKAKLDTGARTSAIHAWDIR
                     PRTIDGEQWVAFTLHPLQRNDRVRKSCLARVVDRRYVTNSGGHRERRYVLETVLRIGK
                     LEWPIELTIANRDEMGFRMLIGRSAMTGRLIVDPSRSYLIGKRKPKRTSVGKRVAATA
                     IRRTVP"
     gene            complement(1720..2073)
                     /locus_tag="VGB88_14055"
     CDS             complement(1720..2073)
                     /locus_tag="VGB88_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9648625.1"
                     /translation="MLRTRLNATLIAAVLAAASPLIAGPGAAAELGSVSNGEAIALEI
                     CSDCHVVSDKQVRVGVVGLPSFRGLANNPEKSELWLRTFMRTPHFEMPDFVFSDDQLD
                     DIIAYFRSLEEKRQD"
     gene            complement(2177..3592)
                     /locus_tag="VGB88_14060"
     CDS             complement(2177..3592)
                     /locus_tag="VGB88_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HEX9648626.1"
                     /translation="MVGRRTRRRIGRAVGLVGVVLALALAAAPVCALDYADKLDILDM
                     LRRGSLTKLETWLAAYQNAFENGRASDRLVDHAYSAFANSDPELAETLDRWLDKRPES
                     YAALLARGTYYAHLVWIVRGTVPADKIRPASRRAMGEFLDLARRDLEAAVARKPRLSA
                     AYARLITAHKWLGNASDAEFVMRKGLDLVPGSFVIRAAYLDSLVPWWRAELTVAKALQ
                     DISGFMKEVEYSAQRYPDLRPLLGYPDFVSGELLSRAGRYDAAGGYLDRALSHGAFWM
                     YHFERGVTFALGGQHVFALQRYDRALALRPQAAEILAQRGWAYWGLDNRSQAIVEWDQ
                     ALALDALEPGTLAAKATALEALGRSDEALAAYDKAVFYGAYDRQVRAGRGTLRLHRFG
                     DFAGAVEDLRVATELAPDQVGYWHDYGLALVKMNACDGDAAAALATYIRLCRDNGGCP
                     EDRLIWAAGTVERITAGDCTE"
     gene            complement(3694..4596)
                     /locus_tag="VGB88_14065"
     CDS             complement(3694..4596)
                     /locus_tag="VGB88_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9648627.1"
                     /translation="MEAWVPITIAAAFFQNLRSALQKHLIGRLSTTGATFSRFLYAAP
                     LAVLYVVGLAALAGKPVPAPNSAFALACGIGGLAQIAATALLLHLFAYRNFAVGTAYS
                     KTETVQTALFGIVILGDAVSLGAAVAILVSLAGVIALSAARTGAGLAALAADIAGKPA
                     LIGLLSGAGFGIAAVSYRSAALSLGGDGFVMRAALTLASVLVFQTLLMGAYIAWREPG
                     QLAAVLRCWRVARWVGVSGMVASVGWFTAMTIQNAAYVRALGQIELVFTFVVSALVFR
                     ERSTLREVAGIGLIVGGIVILLVS"
     gene            4785..5462
                     /locus_tag="VGB88_14070"
     CDS             4785..5462
                     /locus_tag="VGB88_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648628.1"
                     /translation="MSGFARVPVAQLLIAAAIAFVAALSGGAASAQDDPGAIVREHRI
                     TDSVAVVPDWRRPMRSRDSALAEYASVFDCTGQILHQSFKGALESPLRFWFGVRDDDR
                     IGGMVTKVGTPLDLPQQEITGRLSPHYDHDGRFTGGRLLVNWPYANAGQPVELAMMRE
                     TFIRSGPRINGVGKILHRTTKHTYLAGSLSFDPASHASLSSFYRVSFIAATCYPAHDL
                     PELTSAE"
     gene            5539..6162
                     /locus_tag="VGB88_14075"
     CDS             5539..6162
                     /locus_tag="VGB88_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648629.1"
                     /translation="MRAPAGLAAVAVLAAVLSACAGPETEAPPSAAPGAVAALVAPPP
                     PKPAVMIRFDCLGTLLHPPVAGVGESPIKFWLGVREDGTLDGRVDQAGAPLDLGPGPL
                     SGHMSLRYDTAGRLIGGTALVNWPHSRDGRPVELSLMRETFIASGSRANGVGRVRHRA
                     SDHTYVTGGLMLDPQAHPALAGMARVTVLSATCLPSSADERPVAAGR"
     gene            complement(6178..6690)
                     /locus_tag="VGB88_14080"
     CDS             complement(6178..6690)
                     /locus_tag="VGB88_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648630.1"
                     /translation="MANKIAIRIAVAAAMAGAALVAVAAPAAFANRNAILQSLGAGGV
                     ESAAPSADMAAGYQAIDAGDYEAALAAFGTAVEADPGNAEAYNELGYVHRRLQDFDQA
                     FAYYRRALEIDPELTGAHQYIGEAYLEVGNLEMAEYHLRQLDLICLFGCDDYYELEQA
                     VALYKANIAG"
     gene            7302..8897
                     /locus_tag="VGB88_14085"
     CDS             7302..8897
                     /locus_tag="VGB88_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9648631.1"
                     /translation="MAQINKDNVGQLRPAWIFQPGDTRMGSHVTPLAVDGAVYTASNP
                     STVWKLDGATGKRLWSWKPELDQAVVARSFFSHTRGLSVGDGRVYMGTADGRVVALDD
                     LSGEVVWEKQVVDSTKETAGFSGAGTFVSSDLMIIGQNGGEYPVEGKIFGLDPKSGDT
                     KWTFYTTGRGDDRALATWGGDSWKYGGGGSWQAGTIDYTNNQAIFGTSNPNPDYDYCG
                     DQCRDPNADAHRPGDNLYTSSTVALDLDTGKLNWYFQEAPSDAYDYDSAPGEFLILAD
                     DSGRRVVLHPGKNGFDHVYDAKTGQPINVYPDMKNQNWTTGYNLESGQWENILWPKAG
                     EKTLVCPAIDGGHSWNPGSYSPQTGLFYRTVQEWCMWLTVAPKGGADYITAGSEVRTT
                     EPFAQAFMAAEWIGTNPPGDTSHGYITARDPVSGKLAWEKRYDIIPHSALMTTGGGLL
                     FNATTDGYAEALDAETGDLLWRFNNGSGHNGGIISYAAGGKQYVAVVSGHGSYVGRAV
                     ESLNRDKLVNYQESSALVVFALP"
     gene            9013..9450
                     /locus_tag="VGB88_14090"
     CDS             9013..9450
                     /locus_tag="VGB88_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9648632.1"
                     /translation="MTLTTCSLRRLGLALVAILALAAAAGRGAEPARAADDPASAYPN
                     TNPMAGDQEATKEGARLYFKWCVACHGKHADGQETRFGAYGADLTNFWRGYPQFVVIV
                     LNGRTGNIGMMPPWGGVLDEDQISQIGAYLETLATAKARWVGY"
     gene            9492..9938
                     /locus_tag="VGB88_14095"
     CDS             9492..9938
                     /locus_tag="VGB88_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648633.1"
                     /translation="MTKLTLTAVALGLAVAAAIPAQAADPVDDTPPQTLYSGIWLCST
                     PEAYDSAVERAADLPRDQMGPLETELAKTKQCIVVDNDDVEEMMAPFVTVLEQRGGKV
                     LIHYMVEFEKRIELLHRQVTQVMFTGWTDRANLRNYYEWLTGKPQT"
     gene            10001..10411
                     /locus_tag="VGB88_14100"
     CDS             10001..10411
                     /locus_tag="VGB88_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648634.1"
                     /translation="MVGALLLVPAAGGVAVATERGRAMTIEGTIRLAAGAEDALAPGD
                     RLIVKIFHPGEGVEMDPKYQIETDFSLPMAFRIAPPIDMNANARWPDYVVEAFTDRDG
                     DVLSMVAGELYARTPRALALGTAEVVLDLAPHGP"
     gene            complement(10439..11779)
                     /locus_tag="VGB88_14105"
     CDS             complement(10439..11779)
                     /locus_tag="VGB88_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9648635.1"
                     /translation="MTAGTGRTGLAQGAAALLLALAVWLRALPAAAAGETDHASLVGA
                     ALELACRAPAVEDMAQKAALLPGFGGFHVQPRAVSPTGWRVSFEVSGGVLTILRLAPG
                     GELRDVTAQFDSQGGRRPELLASADITCSIRTARRLVYGPDGDLQAIEHLDDLFAPVG
                     WQEPVNPEVPRRRDPGGLAVALIDTGVNYLLPEIEARLARDVDDDLLGFDYWDLDRRP
                     FDADPVESVFFPAHHGTGTASLVAREAPVAKLVPYRYPWPDMARLTDLVADAAAKHIR
                     LVNISLADPEAANWRAFADAAARHTEILFIAAAGNLSRDLDREPMYPAALDLPNLITV
                     TSAMASGRLYQHANWGRQTVDLMVPAFGLRVIDFDGGQRRVSGSSYAAARVSALAACL
                     LAAHPEWTTARLRQAILARAVASADSGQVAVGFLPDPAVTDRGACRAMAQVYGL"
     gene            complement(11788..12621)
                     /locus_tag="VGB88_14110"
     CDS             complement(11788..12621)
                     /locus_tag="VGB88_14110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648636.1"
                     /translation="MTRSLIRVPAALTLLLSLAAFETIDSPYEAVALANRAYAEARYR
                     EAGELYEAALSQLPDSARLHYDYGNAFFRLHDLEKAQAEYMRALDTDDGALAARVHYN
                     LGNVHYQRSLNAMRTFRDAMTPIREAMDNYREALELDPQIADAMYNLELADRLYDELK
                     AQRVQPQANPEARDRKGSANRGQDFEKQADAQREQSDQDQTKREGTPQGEMGEVVPEG
                     TPTAQQTAETTPGGRQRDLTAKEAEQVVEMVRDRARAAEALRQQWREAHMRDEAIERP
                     W"
     gene            complement(12625..13647)
                     /locus_tag="VGB88_14115"
     CDS             complement(12625..13647)
                     /locus_tag="VGB88_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9648637.1"
                     /translation="MQLAAPYFLVVLAAVPALAWLYVFGFRRRRRALAGFVALGLAQS
                     LVGRHSWRRRIVKAACLVAAVGLLALAVTQPQWGRDLGDASRRGRDLFILLDVSQSML
                     AEDVAPNRLEAAKAAIAAFVDDLRQVGGHRLGLVAFAGRASLQSPLTMDYAFFLNRLA
                     QVTTSSVERQGSALGDAILKTVYGYGELDAAHTDLIILSDGEDQGSLPLEAARTAAGE
                     GIALYSVGIGDPAEGARIPITTKEGARQYLRFAGQDVVSVMQEPLLKQLARASKGAYL
                     QARTGPFELNTLFVDHIANKAQRDLEVTEGERLVERYHWFVVIALVLLATEMLFAERP
                     RAEMEA"
     gene            complement(13659..14681)
                     /locus_tag="VGB88_14120"
     CDS             complement(13659..14681)
                     /locus_tag="VGB88_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9648638.1"
                     /translation="MRFENPWLLLLLLLVPVLVQLSRRRGRQATVAYPLVADLGVLGP
                     TLMTRLRAALPYLRAVAIGLCVLALARPQWGQQVTRIYRDGIAIVMVVDTSTSMAALD
                     LQIGDEQANRLEVVKQTFESFVRGDGDGLGGREGDLIGMVTFARYSDNLSPLTLDHAA
                     LLAALEEVKIIQLSEEDGTAIGDAVVMGAELLRRAGSKSKVLVVLTDGSNNAGTADPV
                     QAAMAAKALDVKMYTIGTGTRGVAIGPVRQADGSTLMIPTQVFIDEDTLTQMATATGG
                     KYFRAADAEALAAIYAEIDRLEKSTNIAEQYQRYIEGFPALLLAALTLLLLEGVLVNT
                     RLRALP"
     gene            complement(14678..15793)
                     /locus_tag="VGB88_14125"
     CDS             complement(14678..15793)
                     /locus_tag="VGB88_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648639.1"
                     /translation="MRRARLWLALVAAVAAAAPAWAQVARRVEGGPNLEPGAVEAETV
                     KDKVRVVISADRDALAIVDTLRVTLAVEAPPWILVAFPRLVDELGPFKLVREEALGPF
                     VVPGGDRIERNERRYVLDPVKPGEHSIPPMTLSVVDASKAPSIACVYLNECIRERGDF
                     STTRGPEFIRTGRLLVEVTSVLPADADITQPKDILPPVELPPPPPTEIPWLEIAGAAA
                     ALAVVVLAALWLRRRVRFGPRPKPVPTSPAHELALAALRRLEGAEAGTPERIDAFYVR
                     LSAIVRRYLDWRFGLHAPERTTEEVLRAAMQAVTALAARRDLVGDFLGRCDRVKFARQ
                     RPPGRDMRDMLGTAVRFVEDTADASARVPVAQGGEAL"
     gene            complement(15790..16689)
                     /locus_tag="VGB88_14130"
     CDS             complement(15790..16689)
                     /locus_tag="VGB88_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9648640.1"
                     /translation="MLPAEVYKQVKQVHIRTRRLVDGIFAGEYHSVFKGRGIEFAEVR
                     EYVPGDDVRTIDWNVTARTGQPFVKQYVEERELTVMLLVDMSASGRFGSVARLKHQVA
                     TELAAVIALSAINNNDKIGLILFTDKVERFIAPGKGRNRALRLISELLAFEPERTGTR
                     IASSLEYLHSVLRKRSVTFLISDFFPGEEGDDYERALRLVHRRHDVIPVRLFDRRDTD
                     LPAVGFVRLRDLETGEEVLVDTDSAAVREQFRAGREQAWTQRRHFFRALGIDVIEVRT
                     DQHYVGPLMRFFRQRERRLGEGR"
     gene            complement(16707..17696)
                     /locus_tag="VGB88_14135"
     CDS             complement(16707..17696)
                     /locus_tag="VGB88_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9648641.1"
                     /translation="MPLDVSEIDALVETQSQFVGDLMRAMERKIVGQKYMLERILVGL
                     LANGHVLIEGAPGLAKTRAVVALAEGLHAAYQRIQFTPDLLPADLVGTLIYQPQSGAF
                     TVRKGPVFANLVLADEINRAPAKVQSALLEAMQENQVTIGDESFRLPQPFLVLATQNP
                     LELEGTYPLPEAQIDRFMLKLKITYPSRDEELEILERMATTSPEPPLAAVTDAAAILR
                     ARGVVDEIYLDDKIKHYIVDLVHATREPAAHGLDIGRYIDFGASPRGSIYLALAAKAY
                     AFIHGRGYVTPQDIKSIGMDVLRHRMLLTYEAEAADITTEDLVQQIFDRLPVP"
     gene            complement(17796..19994)
                     /locus_tag="VGB88_14140"
     CDS             complement(17796..19994)
                     /locus_tag="VGB88_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9648642.1"
                     /translation="MPQARKLTTDTEWLEIRATAADWRKADASALLRMLHHLHLIRAF
                     EELVLELAGEALVHGPAHSSIGQEGAAVGCISALRAADQINGTHRAHHHFLAKALGYV
                     DTGKPDPFAGALPEAMQTVLMRTLAEILGLEPGYGRGRGGSMHLRWKDAGVVGTNAIV
                     GGGVPIATGVAWSRKQAGEGDVVVTFFGDGAVHQGAVAESFNLAALWDLPILFFIDNN
                     QYAVSTTIAESTRERRLTARGLAYGIPAYTCDGMDALAVRIATMRALALLRAGKGPAI
                     VEASSYRYFHQNGPLPGSAFGYRSKDEEAEWRARDAIDRLGAEMVRLGHLDQRRLARL
                     RTDAQGSVARAGETLLEPEGNRRRIRPALWPDPATVDHGIRGDLSELAGARFVELETF
                     AGAVEERKFVDLVAAVMGRRMATDERIYVLGEDVHHLRGGTNGATRGLAERFPGRIIG
                     TPITEGGFAGVGGGIAADGKFRPVVEFMYPDFCYVAADQVFNQIPKMRHMFGGDMAVP
                     LVLRGKVAAGSGYGSQHSMDPIGLYAMFPGWRIVAPSTPFDYVGLMNSALACDDPVLV
                     VEHLDLYGATGPAPVDDLDYFIPLGKAKVVRPGEDFTVLASLSMVRPCVEVADRLGVS
                     AEVIDLRSLDRAGLDWQTIGGSVAKTNNVLIVEQGTRGTSWGGPVADEIQRRLFDYLD
                     QPVQRVCGAESAPTISKSLDQAANAGAGDIERAYREVLAATGRPYAQAAE"
     gene            complement(20065..20952)
                     /locus_tag="VGB88_14145"
     CDS             complement(20065..20952)
                     /locus_tag="VGB88_14145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648643.1"
                     /translation="MAEAVGFVGLGNMGAPIARRLIDAGHAVRVYNRTAAKAAPLVEA
                     GAKRAEAPGGVAAKGGVVLSIVSDDAALEEIALGPDGVLAALGPGGVHVSMSTVAVAT
                     ARRMEALHAEAGATYLCAPVFGRPAAAAAGKLWVALSGQKAAKVRVAPLLGAFSQGVR
                     DFGDAPGAANVVKLSGNFLIGAAIEAISEACALAEKNGVDRAAFVELLSGSLFDCPVY
                     RIYGEGIAKRQYEPPGFRLSLGLKDVNLVLAAAAAAEVPMPLASLVHDRLLASVAKGR
                     SHLDWMAMDLSVSEDAGTK"
     gene            21057..21509
                     /locus_tag="VGB88_14150"
     CDS             21057..21509
                     /locus_tag="VGB88_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9648644.1"
                     /translation="MAGKVLGTNHTSFTVSDLDRAIQFYTQALGFRLLHRGGRDAAMI
                     EKVVGVPGADIEVAYLQAPGHRMELIRYLGPADRGRVVSRPCDTGFAHVAFDVDDVDA
                     VLAAAARHGVKPVGPVQDLHHGPNAGGRVVYTRDPDGITVEFIEQPKG"
     gene            complement(21506..22300)
                     /locus_tag="VGB88_14155"
     CDS             complement(21506..22300)
                     /locus_tag="VGB88_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648645.1"
                     /translation="MTKAEIERLQGLLNKVGGLLATDGQIGRNTRRAVADARALSGLP
                     GGTEADQALIDWLAAQAEPSPDLPTEGVTFIANEEVGGRDFYEAQATFPQWPGEQSGI
                     TIGVGYDLRFSADIFETDWGDKLPADVLAALRPHLGKLGNRAAAEALSGLRVPWTTAW
                     RVFIGRSLPLQVVRTRGVYTAFANLPGLCRSVLVSLVFNRGTDLDDEPGSDRRLEMRT
                     IRGLLQGGKVDQVPDQLLAMRRLWPDSRGLRERREREAALWRKGLA"
     gene            complement(22303..22872)
                     /locus_tag="VGB88_14160"
     CDS             complement(22303..22872)
                     /locus_tag="VGB88_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9648646.1"
                     /translation="MNAPGLERRAVLRLAGTALAGAALGRAFPLGAALAAGRIDARLA
                     ELGVELPAAAAPVANYVGWVRTGNLVFVAGQGPILDGEVKYKGRVGADLTIEQGQAAA
                     RLCALNVLAQLKAACDGDLDRVVRCVKVGGFVNSAPDFFDQPKVVNGASDLFVEVFGD
                     AGRHARFAVGTSVLPFNIAVEVDAHFEVS"
     gene            complement(22899..>23208)
                     /locus_tag="VGB88_14165"
     CDS             complement(22899..>23208)
                     /locus_tag="VGB88_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9648647.1"
                     /translation="LAREGIEVEVIDPRTLKPLDEATILDSVRKTNRLLIVHEAWRTG
                     GFGAEVAALAVEKVFDYLDAPIARLGALDTPMPYNDELERAAIPSQEKIVEAVRALVT
                     "
BASE COUNT         3482 a         8305 c         8037 g         3384 t
ORIGIN      
        1 cggatgatct cgacgccgtt gatctcgtcg ccgtgcagcg ccgcgcacac caacagccgc
       61 gggccgtcgc ggcggccgtg caggacctgg accggcagcc cgatcggggt gtgggtcgac
      121 agctcggcga tcttgagctc gatcaccgcc cggccgcccg ccttgacgtg atggccgccg
      181 accaccaggt cgggccgacc gccgtcagcc tttgccgcgg gtgcgggtgc ggtgcggtct
      241 ggcatgcttc tcgacgaact cgatgacctt gccggcgatg tcgacttcgg tcgccgcctc
      301 gatgccctcg agcccgggcg aggagttgac ctccatcacc accggcccgt ggttcgagcg
      361 cagcatgtcg acgccggcga cgttgagccc catggcgcgc gccgcgcgca ccgcggtcga
      421 gcgttcctcg ggcgtgatgc ggatgcgcgc cgcgctgccg ccgcgatgga tgttggagcg
      481 aaactcgcct tcgggcgcct tgcgctgcat cgcggcggcc acgcggtcgc cgatcacgaa
      541 ggcgcggatg tcggcgccgc cggcctcctt gatgtactcc tggaccagga tgttgacctt
      601 ggcgccgcgg aacgcctcga tcacgctctt ggccgagttg tgggtctcgc cgaggaccac
      661 gccgagccct tgcgtgccct cgagcagctt gaccaccacc ggcgcgccgc cggccatctc
      721 gatcaggtcg tcggtgtaac gggtggcgtg ggcgaagccg gttaccggca ggccgatgcc
      781 ttggcgcgca agcagctgga gcgagcgcag cttgtcgcgc gaccgcccga tcgcgaccga
      841 ttcattcagc ggccacaccc ccatcatctc gaactggcgc agcaccgcca ggccgtagaa
      901 ggtgatcgag gcgccgatgc gcgggatcac cgcatcgatg cccgacagca cctcgccgcc
      961 atagcgcacc tccgggcggt gggcggtgat gttcatgtag cacatgagcg tgttgtagac
     1021 ctcgatatcg tggccgcgct ggcgcgccgc cgcgatcagg cgctggtgcg agtacaggcc
     1081 tggattgcgg gccagcatgg cgatcttcac ggaacggttc tccttatcgc cgttgcggcg
     1141 acgcgcttgc cgaccgacgt ccgcttgggc ttgcgcttgc cgatcaggta ggaccgggac
     1201 ggatcaacga tcagcctgcc ggtcattgcc gagcgtccga tcagcatgcg aaagcccatc
     1261 tcgtcgcggt tggcgatggt gagttcgatc ggccattcga gcttgccgat ccgcaacacc
     1321 gtctcgagga cgtagcggcg ctcgcggtgg ccgccggagt tggtgacgta gcgccggtcc
     1381 acgacccgcg ccaggcacga ctttctgacc cgatcgttgc gttgcagcgg gtgcagggtg
     1441 aaggcgaccc actgctcgcc gtcgatcgtc cgcggccgga tgtcccaggc gtgaattgcc
     1501 gaggtgcgcg cgccggtgtc cagcttggcc ttgacctttt ccaccccgag cgccggcaat
     1561 gccacccatt cgcgccagcc caccgcgtgc cggtgctcgt cgtccatcgc ccgccgttcc
     1621 ctcacgccgc gacaccgcca gcgccggtca tagcgaaccc ggcgcgccgc cgcaacggcg
     1681 cgcgcgaccc ctcgcgtgcc gccgccggcg aacgcgcggt cagtcctggc gcttttcctc
     1741 gaggctccga aagtaggcga tgatgtcgtc gagctggtcg tcgctgaaca cgaagtcggg
     1801 catctcgaaa tgcggcgtgc gcatgaacgt gcgcagccag agctcggact tctccgggtt
     1861 gttggccaga ccgcggaagc tcggcaggcc gaccacgccg acccgcacct gcttgtccga
     1921 gacgacgtgg cagtccgagc agatctcgag cgcgatcgcc tcgccgttgc tgacgctgcc
     1981 gagttccgcg gccgcccccg gcccggcaat caacgggctc gccgccgcca gcacggcggc
     2041 aataagggtg gcattcaatc gagttcgtag catgaccatt gagccctcgt ggattcggtt
     2101 gcaacgctgc cgccggaagc ggccggtggc cataacctgt atcatggacg gcgcgcggcc
     2161 gtgggcggcg acgacgttac tcggtgcagt cgccggcggt gatccgctcg accgtgccgg
     2221 ccgcccagat cagccggtcc tcggggcacc cgccgttgtc ccggcacagg cggatatagg
     2281 tggcgagcgc cgccgccgca tccccgtcgc aagcgttcat cttgaccagc gccagcccgt
     2341 aatcgtgcca gtagccgacc tggtcgggcg cgagctcggt ggcgacgcgc aggtcctcga
     2401 ccgcgcccgc gaagtcgccg aaccggtgca gcctgagggt gccgcgcccg gcgcgcacct
     2461 ggcggtcgta ggcgccgtag aagacggcct tgtcgtaggc ggccaaggcc tcgtcggagc
     2521 ggcccagcgc ttcgagcgcg gtcgccttgg cggcgagcgt ccccggctcg agcgcatcga
     2581 gcgccagcgc ctgatcccat tcgacgatcg cctgcgagcg attgtccaat ccccaatacg
     2641 cccagccccg ctgggccagg atctcggcgg cctggggacg caacgccagc gcccggtcgt
     2701 atctctgcag cgcgaacacg tgctggccgc cgagggcaaa ggtgacgccg cgctcgaagt
     2761 gatacatcca gaaggcgccg tggctgagcg cacggtcgag atagccgccg gcggcgtcgt
     2821 agcgcccggc ccgcgacagc agctcgccgc tgacgaagtc ggggtagccg agcagcggcc
     2881 gcaggtcagg gtaccgctgg gcgctgtatt cgacctcctt catgaacccg gagatgtcct
     2941 ggagcgcctt tgccaccgtc agctcggcgc gccaccacgg caccaggctg tcgagatagg
     3001 cggcgcggat gacgaacgag ccgggcacca ggtcgagccc cttgcgcatc acgaactcgg
     3061 cgtcgctcgc gttgcccagc cacttgtggg cggtaatcag gcgcgcatag gcggcgctga
     3121 gccgcggctt gcgcgccacc gccgcctcga ggtcccggcg cgcgaggtcg aggaactcgc
     3181 ccatcgcccg ccggctcgcc gggcgaatct tgtccgcggg caccgtgccg cgcacgatcc
     3241 agaccaggtg cgcgtaatag gtgccgcgcg cgagcagcgc ggcgtacgac tccgggcgct
     3301 tgtccagcca gcggtcgagg gtctcggcaa gctcgggatc ggagttggcg aaggcgctgt
     3361 aggcgtggtc gaccaggcgg tccgaggcgc gcccgttctc gaacgcgttc tgataggccg
     3421 caagccaggt ctcgagcttg gtcagcgacc cgcgccgcag catgtcgagg atgtcgagct
     3481 tgtcggcata atcgagggcg cacaccggcg ccgcggcgag tgccagcgca agcaccaccc
     3541 cgaccaggcc gacggcccgg ccgatccgcc gtcgcgtccg ccgccctacc atgggcccgc
     3601 ggtgcgccgc ccgcaggtga tcggcattgt gcccgtgacc gacatcggcg ccattattcc
     3661 agcgcgacgg tcaaccgcaa gcgcggcgcg cagtcacgac accaacagga tcacgattcc
     3721 gccgacgatc aggccgatgc cggcaacctc gcgcagggtc gagcgctcgc ggaacaccag
     3781 ggccgagacg acgaaggtga acaccagctc gatctggccg agggcgcgaa cataggccgc
     3841 gttctggatg gtcatggcgg tgaaccagcc gacgctggcc accatgccgg acacgccgac
     3901 ccacctcgcg acccgccagc agcgcagcac ggcggcaagc tggccgggct cgcgccacgc
     3961 gatataggcg cccatcaaca gggtctggaa taccagcacc gaggcgagcg tcagcgccgc
     4021 ccgcatgacg aagccgtcgc cgcccagcga cagcgccgcc gagcggtagg acacggcggc
     4081 gatgccgaag ccggcccccg acaacagccc gatcagcgcc ggcttgccgg cgatgtcggc
     4141 ggcgagcgcg gccagcccgg cgccggtgcg cgccgccgac agcgcgatca ccccggccag
     4201 gctgaccagg atcgccaccg cggcgcccag gctgaccgca tcgcccagga tgacgatccc
     4261 gaacagcgcc gtctgcaccg tctcggtctt cgagtacgcg gtgccgacgg caaagttgcg
     4321 gtaagcgaac aaatgcagca gcaatgccgt cgccgcgatc tgggcgaggc cgccgatgcc
     4381 gcaggcgagc gcgaaggcgg aattcggcgc cggcaccggc ttgccggcaa gggcggcgag
     4441 gccgaccaca tagagaaccg cgagcggggc ggcgtaaaga aagcgcgaga aggtcgcgcc
     4501 ggtggtgctg agccggccga tcaggtgctt ctggagcgcc gagcgcaggt tctggaagaa
     4561 cgccgcagcg atcgtgatcg gaacccacgc ctccattcga ccccctgcgc accccggcaa
     4621 ggcgcgcaat agaccaagaa aagcagcctg ccggcaaggc caaccgcgaa ctccgcgcgg
     4681 cacgcggggg tgcgcacgca atggtcacaa atttgtcccg gccgcgaggt tggcccgcgg
     4741 atacgctcgg cctaactcca gtcttcggga gagaacggga ccagatgtcg ggtttcgcgc
     4801 gcgtacccgt tgcacaactt ttaattgcag cggcgatcgc attcgtcgcc gcattgagcg
     4861 gcggcgcggc ctccgctcag gacgatcccg gcgcgatcgt gcgcgagcac cggatcaccg
     4921 actcggttgc cgtcgtcccc gactggcgcc ggccgatgcg atcgcgcgac agcgcgctgg
     4981 ccgagtacgc gagcgtgttc gactgcaccg gccagatcct gcaccagagc ttcaagggcg
     5041 cgctcgaatc cccgctcagg ttctggttcg gggtgcgcga cgacgaccgc atcggcggca
     5101 tggtgaccaa ggtcggcacg ccgctggacc tgccccagca ggagatcacc ggcaggctct
     5161 cgccccatta cgaccacgac ggccgcttca ccggcgggcg cttgctggtc aactggccct
     5221 atgccaatgc cggccagccg gtcgagctcg ccatgatgcg cgagaccttc atccgctcgg
     5281 ggccgcggat caacggcgtc ggcaagatcc tgcaccgcac caccaagcac acctatctgg
     5341 ccggaagcct gtcgttcgac ccggccagcc acgcctcgct gtcgtcgttc tatcgcgtct
     5401 cgttcatcgc cgcgacctgc tacccggccc acgacctgcc cgagctgacc agcgccgagt
     5461 agtcggcgcg aggcgcccgg cgccggcacc ggcggccgcc ggcttccaga ccgcgaagat
     5521 agcgactagg gtggccccat gcgcgctcct gccggactcg ccgcggtcgc ggtccttgcc
     5581 gccgtgctgt cggcctgcgc cgggcccgag accgaggcgc cgccgtccgc agccccgggc
     5641 gcggtcgcgg cgctggtggc gccgccgccg cccaagccgg cggtgatgat ccggttcgat
     5701 tgcctcggca cgctgctgca cccgcctgtc gccggcgtcg gcgaatcccc gatcaaattc
     5761 tggcttggcg tgcgcgagga cggcacgctc gacggccgcg tggaccaggc cggcgcgccg
     5821 ctcgacctcg gcccgggccc gctgtcgggc cacatgagct tgcgctacga caccgccggc
     5881 cgcctgatcg gcggcaccgc cctggtcaac tggccgcaca gccgcgacgg ccgcccggtg
     5941 gagctgtccc tgatgcgcga gaccttcatc gcctcgggca gccgcgccaa cggcgtcggc
     6001 cgggtccgcc accgcgccag cgaccacacc tacgtgaccg gcgggctgat gctcgatccc
     6061 caggcgcacc cggcgttggc cgggatggcc agggtcacgg tgctgtccgc gacctgcctg
     6121 ccgagcagcg ccgacgagcg gccggttgcc gccggccgtt gacacggcgc cggcggctca
     6181 gccggcgata ttcgccttgt acaacgccac cgcctgctcg agctcgtaat agtcgtcgca
     6241 gccgaacagg cagatcaggt cgagctgacg caggtggtac tcggccatct ccaggttgcc
     6301 gacctcgaga taggcctcgc cgatgtactg gtgcgcgccg gtcagctcgg gatcgatctc
     6361 gagcgcgcgc cggtagtagg cgaacgcctg gtcgaagtcc tgcagccggc gatgaacata
     6421 gccgagctcg ttgtaagcct cggcgttgcc gggatcggcc tcgaccgccg tcccgaacgc
     6481 cgccagcgcg gcctcgtagt cgccggcgtc gatcgcctgg tatccggccg ccatgtcggc
     6541 gctcggtgcc gcgctctcga ccccgccggc accaagcgat tgcaggatcg cattgcggtt
     6601 ggcgaaggcc gccggtgcgg ccacggcgac cagcgcggca ccggccatcg ccgcggcgac
     6661 ggcgatccgg atcgcgatct tgttggccat cttgcctgcc tccctgctgg cacgcggccc
     6721 cggccgccag cccgggcgga gcgtccgggg accggggccc cccatagata atatctcggc
     6781 gcggcgctgg ctacctcgtc gccgccgggg ccgccgcctg agatgcccgc ggcgcgatcg
     6841 tccgggctcg acgtgcgacc tctcaaggtc ttggtcgcac cacttcaagc gccggcaaaa
     6901 ggacggcgcg gtggcaactc acttgacagg ggaatgccat cccccgatga tccgacctgg
     6961 aggatttcct ggtcgtcggg ggcattggcc gcatcgtccg cgtcggacgt cgctgctgcc
     7021 gtcgcgtggt cccgatccgg tgtcgccgcg acaggcgtgc ggctcggcca ggagatggcg
     7081 cccggtgcgc cgggcgcgag ttcagcaatg tgtctcaagg gaggtcaccc aagatgaaat
     7141 cagggcaagt cctacacgtg acggcgctcg ccgccgtcat tggggctttc gctgcgacgt
     7201 cggccctcgc ggatagcaac gccgcgcgga tccggcactg cccggacagc gactggtgcc
     7261 attaccatcg caccgtcgac accggctggc ggcacagccc cttggcccag atcaacaagg
     7321 acaatgtggg ccagcttcgc ccggcctgga tcttccagcc cggcgatacg cgcatgggca
     7381 gccatgtcac cccgctcgcg gttgacggcg cggtctatac cgccagcaac ccttcgaccg
     7441 tgtggaagct tgacggcgcc accggcaagc ggctgtggtc gtggaagccc gagcttgacc
     7501 aggcggtcgt ggcacggtcg ttcttctcgc atacccgcgg gctctcggtc ggcgacgggc
     7561 gcgtctacat gggcaccgcc gacggccgtg tggtcgcgct cgacgacctg tcgggcgagg
     7621 tggtctggga gaagcaggtc gtcgactcga ccaaggagac ggcgggcttc tcgggtgccg
     7681 gcacgttcgt cagctcagat ctgatgatca tcggccagaa cggcggcgag tacccggtcg
     7741 aaggcaagat cttcgggctc gacccgaaga gcggcgacac caagtggacg ttctacacca
     7801 ccggccgcgg cgacgatcgg gcgctggcca cctggggtgg cgattcgtgg aagtacggcg
     7861 gcggcggctc gtggcaggcc ggcacgatcg actacaccaa caaccaggcg atcttcggca
     7921 ccagcaaccc gaacccggac tacgactact gcggcgacca gtgccgcgat ccgaacgccg
     7981 acgcgcaccg tcccggcgac aacctgtaca cctcgtcgac ggtcgccctc gacctcgata
     8041 ccggcaagct caactggtac ttccaggaag ccccgtcgga cgcgtacgac tacgactcgg
     8101 cgcccggcga gttcctgatc ctggccgacg acagcggccg gcgcgtggtc ctgcatcccg
     8161 gcaagaacgg gttcgaccac gtctacgacg ccaagaccgg ccagccgatc aacgtctatc
     8221 cggacatgaa gaaccagaac tggaccaccg gctacaactt ggagtccggc cagtgggaga
     8281 acatcttgtg gccgaaagcg ggcgagaaga ccctggtctg ccccgccatc gacggcggcc
     8341 acagctggaa cccgggcagc tacagcccgc agaccggcct gttctaccgc acggtccagg
     8401 agtggtgcat gtggctgacc gtggcgccga agggcggcgc ggactacatc accgccggct
     8461 cagaggtgcg caccaccgag ccgttcgccc aggcgttcat ggccgcggag tggatcggca
     8521 ccaaccctcc gggcgatacc tcgcacggct acatcaccgc gcgtgacccg gtgtcgggca
     8581 agctggcgtg ggagaagcgt tacgacatca tcccgcactc ggcgctgatg accaccggcg
     8641 gcggcctgct gttcaacgcc accaccgacg gctacgccga ggcgctggac gcggagaccg
     8701 gcgacctgct gtggcggttc aacaacggct ccggccacaa cggcggcatc atttcctacg
     8761 ccgccggcgg caagcagtat gtggcggtgg tcagcggcca cggctcatat gtcggccgcg
     8821 cggtcgaaag cctcaatcgc gacaagctgg tcaattacca ggagagctcg gcgctggtcg
     8881 tgttcgccct gccgtaagcg acgacctttt tcggcggggc aaggggttag cccccttgcc
     8941 ccgccttttt ttgtgcatta tctgtctggc tcgccctgtg gcagactatc aacacctaca
     9001 ggagattcgc ttgtgacctt gacgacgtgc agcctacggc gcctggggct ggcgctggtc
     9061 gcgatcctgg cgttggcggc ggcggcgggc cgcggagccg agcccgcgcg tgccgccgac
     9121 gatccggcgt ccgcctatcc gaacacgaac ccgatggccg gcgaccagga ggcgaccaag
     9181 gagggcgcgc gcctgtattt caagtggtgc gtcgcctgcc acggcaagca cgccgacggc
     9241 caggaaacac ggttcggcgc ttacggcgcg gacttgacca atttctggcg cggctatccc
     9301 cagttcgtgg tcatcgttct caacggccgc accggcaata tcggcatgat gccaccgtgg
     9361 ggcggcgtgc tcgacgagga ccagatttcc cagatcggcg cctatctcga aactctggcg
     9421 accgccaagg cgcgctgggt cgggtactga tcgcgcctcg gccgcaaccg gacacgtttg
     9481 aaggagatct catgaccaag ctgacactca ccgccgtggc gctcggactg gccgtcgccg
     9541 ccgcgatccc cgcgcaggcc gccgatccgg tggacgatac ccccccgcag accctctata
     9601 gcgggatctg gttgtgctcc acgcccgagg cttacgactc ggcggtcgag cgcgccgccg
     9661 acctgccgcg cgaccagatg gggccgctcg agaccgagct ggcaaagacc aagcagtgca
     9721 tcgtcgtcga taacgacgac gtcgaggaga tgatggcgcc gttcgtcacc gtgctcgagc
     9781 agcgcggcgg caaggtgttg atccattaca tggtcgagtt cgagaagcgg atcgagctgc
     9841 tgcaccgcca ggtgacccag gtcatgttca ccggctggac cgacagggcg aacctgcgca
     9901 actactacga gtggctgacc ggcaagccgc agacgtgagg ccgcgcggtc gggccgggcg
     9961 agggaacgtc ccggcccttg cccggcgagc ggcggcggcc gtggtcggcg cgctgctgct
    10021 ggtgcccgcg gccggcggcg ttgcggtcgc gaccgagcgg ggccgcgcga tgaccatcga
    10081 gggcaccatt cgcctggccg ccggcgccga ggacgcgctc gctcccggcg accggctgat
    10141 cgtcaagatc ttccatcccg gcgagggagt cgagatggac cccaaatacc agatcgagac
    10201 cgatttcagc ttgccgatgg cgttccgcat cgcgccgccg atcgacatga acgccaatgc
    10261 ccggtggccc gactatgtgg tcgaggcatt caccgaccgc gacggcgacg tgctgtcgat
    10321 ggtcgccggc gagctctatg cacgcacccc ccgggcgctc gcgctcggga ccgccgaggt
    10381 ggtcctggac ctcgccccgc acggcccttg aggccgcgac gcgcgcgccg cctccgggct
    10441 acaggccata gacctgcgcc atggcacggc aggcgccgcg gtcggtcacc gccggatcgg
    10501 gcaggaagcc gaccgcgacc tggccgctgt cggcgctggc gacggcgcgc gcgaggatcg
    10561 cttgcctgag ccgcgccgtg gtccattcgg gatgcgccgc cagcaggcac gcggccagcg
    10621 cgctgacccg cgccgcggcg tagctcgagc ccgacacccg gcgctggcca ccgtcgaagt
    10681 cgatcacccg cagcccgaaa gccggcacca tcaggtccac cgtctgccgc ccccagttgg
    10741 cgtgctggta gagccgcccg ctcgccatcg ccgaggtgac cgtgatcagg ttcggcaggt
    10801 cgagcgccgc cggatacatc ggctcgcggt cgaggtcgcg cgacaggttg ccggcggcgg
    10861 cgatgaacag gatctcggtg tggcgcgcgg cggcgtcggc gaacgcgcgc cagttggcgg
    10921 cctccggatc ggccagcgag atgttgacca ggcggatgtg cttggccgcc gcgtcggcca
    10981 ccagatcggt caggcgcgcc atgtccggcc atgggtagcg gtagggcacc agcttcgcca
    11041 ccggcgcctc gcgcgcgacc aggctggccg tgccggtgcc gtggtgggcg ggaaagaaca
    11101 ccgattccac cgggtcggcg tcgaacgggc ggcggtcgag gtcccaatag tcgaagccca
    11161 gcaggtcatc gtcgacatcg cgcgccagcc gcgcttcgat ctcgggcagc aggtagttga
    11221 ccccggtgtc gatcagcgcc accgctagcc cgcccgggtc gcgccggcgc ggcacctccg
    11281 gattgaccgg ctcctgccag ccgaccggcg cgaacaggtc gtccaggtgc tcgatcgcct
    11341 gcaggtcgcc gtccgggccg tagaccagcc ggcgcgcggt gcggatggaa caggtgatat
    11401 cggcgctggc gagcagctcg ggccgccgtc cgccctggct gtcgaactgc gcggtgacgt
    11461 cgcgcagctc gccgccgggg gccagccgca ggatggtcag cacgccgccc gatacctcga
    11521 agctgacccg ccagccggtc ggcgagaccg cgcgcggctg gacatggaag ccgccgaagc
    11581 ccggcagcag ggcggccttc tgggccatgt cctcgaccgc gggcgcacga caggcgagct
    11641 cgagcgcggc gcccaccagc gacgcatgat ccgtctcccc ggccgccgcg gccggcaacg
    11701 cccgcagcca cacggcaagc gccagcagca gggcggcagc gccctgcgcc aatcccgtcc
    11761 tgcccgtgcc ggcggtcacg ctcgccctca ccagggccgt tcgatcgcct cgtcgcgcat
    11821 atgcgcctcg cgccactgct ggcggagcgc ttcggcggcc cgcgcccggt cgcggaccat
    11881 ctccaccacc tgctccgcct ccttggcggt caggtcgcgc tgcctgccgc cgggagtggt
    11941 ctcggccgtt tgctgcgcgg tcggggtgcc ttccggcacc acctcgccca tctcgccctg
    12001 cggcgtccct tcgcgcttcg tctggtcctg gtcgctttgc tcgcgctgcg cgtcggcctg
    12061 cttctcgaag tcctggccgc ggttggcgga gcccttcctg tcgcgtgcct cggggttggc
    12121 ctgcggctgc acccgctggg ccttgagctc gtcgtaaagg cggtccgcga gctccagatt
    12181 gtacatcgca tcggcgatct gcgggtcgag ctcgagcgcc tcgcggtagt tgtccatcgc
    12241 ctcgcggatc ggggtcatgg cgtcgcggaa ggtgcgcatc gcattcagcg accgctgata
    12301 gtggacgttg ccgagattgt agtggacccg ggcggcgagg gcgccgtcgt cagtgtcgag
    12361 cgcccgcatg tactcggcct gcgccttctc cagatcgtga aggcggaaga acgcattgcc
    12421 gtaatcgtag tgcagccggg ccgagtccgg cagctggctc agcgccgcct cgtacagctc
    12481 gccggcctcg cgatagcgcg cctcggcata ggcgcggttg gccagcgcca ccgcctcgta
    12541 aggcgagtcg atggtctcga atgccgccag cgacaatagc agcgtcagcg ccgccggcac
    12601 ccgtatcagc gatcgggtca tggcctaggc ctccatctcc gcgcgcggcc gctcggcgaa
    12661 cagcatttcg gtcgccaaca gcaccagcgc gatcaccacg aaccaatggt agcgttcgac
    12721 caggcgctcg ccctcggtaa cctcgaggtc gcgctgcgcc ttgttggcga tatggtcgac
    12781 gaacagcgtg ttcagctcga acggcccggt gcgggcctgc agataggcgc ccttgctcgc
    12841 ccgcgccaat tgcttgagca gcggctcctg catcaccgag accacatcct ggccggcgaa
    12901 gcgcagatat tggcgcgccc cctccttggt cgtgatcggg atgcgcgcgc cctcggcggg
    12961 gtcgccgatg ccgaccgagt agagcgcgat gccctcgccg gccgcggtcc gcgccgcctc
    13021 cagcggcagg ctgccctggt cctcgccgtc gctgaggatg atcaggtcgg tatgggccgc
    13081 gtccagctcg ccgtagccgt aaaccgtctt gaggatggcg tcgccgagcg cgctcccctg
    13141 gcgctcgacg ctgctggtcg tcacctgagc gagccgattg aggaagaacg catagtccat
    13201 ggtcagcggg ctttgcaggc tggcgcggcc ggcgaacgcg accagcccca ggcgatggcc
    13261 gccgacctgt ctcagatcgt cgacgaacgc ggcgatcgcc gccttggccg cctccagccg
    13321 gttcggcgcg acgtcctcgg cgagcatgct ttgcgagacg tcgagcagga tgaacaggtc
    13381 gcggccgcgg cgcgacgcgt cgcccaggtc gcggccccat tgcggctggg tgaccgccag
    13441 cgccagcagg ccgaccgcag ccaccaggca cgcggccttg acgatccgcc gccgccagct
    13501 gtggcggccc accaggcttt gcgcgagccc cagcgcgacg aaaccggcaa gcgcccgccg
    13561 gcggcggcgg aacccgaaaa cgtagagcca cgccaacgcc gggaccgccg ccagcacgac
    13621 caggaaatag ggcgctgcga gctgcattcc gggcgcgctc agggaagcgc cctcaggcgc
    13681 gtgttgacca gcacgccctc gagcagcagc agggtgagcg cggccaaaag cagcgccggg
    13741 aagccctcga tatagcgctg gtactgctcg gctatgttgg tcgatttctc cagcctgtcg
    13801 atctcggcgt agatcgccgc cagcgcctcg gcgtcggcgg cacggaagta cttgccgccg
    13861 gtggcggtcg ccatctgggt gagcgtgtcc tcgtcgatga acacctgggt cggtatcatc
    13921 aaggtgctgc cgtcggcctg gcgtaccggc ccgatcgcca cgccgcgcgt gccggtgccg
    13981 atggtgtaca tcttgacgtc gagcgccttc gccgccatcg ccgcctggac cgggtcggcg
    14041 gtgccggcgt tgttgctgcc gtcggtcagc accaccagca ccttgctctt gctgccggcg
    14101 cggcgcagga gctcggcgcc catcaccacc gcgtcgccga tcgcggtgcc gtcctcttcg
    14161 gacagctgga tgatcttgac ctcctccagt gccgccagca acgccgcgtg gtcgagggtc
    14221 agtgggctca ggttgtcgct gtagcgggcg aaggtgacca tgccgatcag gtcgccctcg
    14281 cgcccgccca ggccatcgcc gtcgccgcgc acgaacgact cgaaggtctg cttgaccacc
    14341 tcgagccggt tggcctgctc atcgccgatc tgcaagtcga gcgcggccat gctggtcgag
    14401 gtgtcgacca ccatgacgat ggcgatgccg tcgcggtaga tgcgggtcac ctgctggccc
    14461 cattgcgggc gcgccaacgc cagcacgcac aagccgatcg cgaccgcgcg cagatacggc
    14521 agcgcggccc tgagccgggt catcagcgtc ggccccagca cgcccaggtc ggcgaccagc
    14581 ggataggcca cggtcgcctg ccggccgcgc cggcgcgaca gctggaccag caccggcacc
    14641 aacagcagca gcagcagcag ccaggggttc tcgaacctca cagcgcctcg cctccctgcg
    14701 ccaccggcac ccgcgcgctg gcgtcggcgg tgtcctcgac aaagcgcacc gcggtgccga
    14761 gcatgtcgcg catgtcgcgt ccgggcgggc gctggcgggc gaacttgacc cggtcgcagc
    14821 ggccgaggaa gtcgccgacc aggtcgcggc gggcggcgag cgcggtcacc gcctgcatcg
    14881 ccgcgcgcag cacctcctcg gtggtgcgct cgggcgcgtg caggccgaac cgccaatcga
    14941 ggtagcgcct gacgatcgcc gacaggcgca cgtaaaaggc gtcgatgcgc tcgggcgtgc
    15001 cggcctcggc gccctcgagc cgcctgagcg cggcgagcgc cagctcgtgc gccggcgagg
    15061 tcggcaccgg cttcggccgc ggcccgaacc tgacccgccg gcgcagccac agcgccgcca
    15121 gcaccacgac cgcaagcgcg gccgcggcgc cggcgatctc gagccaggga atctcggtcg
    15181 gcggcggcgg cggcagctcg accggcggca ggatgtcctt gggctgggtg atgtcggcat
    15241 ccgccggcag caccgaggtc acctcgacca gcagcctgcc ggtgcggatg aactccggtc
    15301 cgcgggtggt cgagaaatct ccccgttcgc gtatgcactc attcaaatag acgcaggcga
    15361 tcgacggcgc cttgctggcg tcgaccaccg acagggtcat cggcggaatc gagtgctcgc
    15421 cgggcttgac cgggtcgagt acgtagcggc gctcgttgcg ctcgatgcgg tcgccgccgg
    15481 gaaccacgaa cgggccgagc gcctcctcgc gcaccagctt gaacggccca agctcgtcga
    15541 ccaggcgcgg aaacgcgacc aggatccagg gcggcgcctc gaccgcgagg gtgaccctga
    15601 gcgtatcgac gatcgccaac gcgtcgcggt cggcggagat gaccacgcgc accttgtcct
    15661 tgaccgtctc cgcctcgacc gcgccgggct cgaggttcgg cccgccctcg acccggcggg
    15721 cgacctgggc ccaggcgggc gcggccgcgg cgacggcggc gaccagcgcc agccaaagcc
    15781 gggcccgcct caccgcccct cccccagccg gcgttcgcgc tggcggaaga agcgcatcag
    15841 cgggccgaca taatgctggt cggtgcgcac ctcgatcacg tcgatgccga gcgcgcggaa
    15901 gaagtgccgg cgctgcgtcc aagcctgctc gcgcccggcc cggaactgct cgcgcaccgc
    15961 ggcgctgtcg gtgtcgacca gcacctcctc gccggtctcg agatcgcgca ggcgcacgaa
    16021 gccgaccgcc ggcaggtcgg tgtcgcggcg gtcgaacagg cgcaccggga tcacgtcgtg
    16081 acgccggtgg accagccgca gggcgcgctc gtagtcgtcg ccctcctcgc ccggaaagaa
    16141 gtccgagatc aggaacgtga ccgagcgctt gcgcagcacc gaatggaggt actccaggct
    16201 ggacgcgatc cgggtgccgg tgcgctcggg ctcgaacgcc agcagctcgc tgatcagccg
    16261 cagcgcccgg ttcctgccct tgccgggcgc gatgaagcgc tcgaccttat cggtgaacag
    16321 gatcagcccg atcttgtcgt tgttgttgat cgcgctcagc gcgatgaccg cggcaagctc
    16381 ggtcgcgacc tggtgcttga gccgggcgac gctgccgaac cggcccgagg cgctcatgtc
    16441 gaccagcagc atgacggtga gctcgcgctc ctcgacgtac tgcttgacga acggctggcc
    16501 ggtgcgcgcg gtgacgttcc agtcgatggt gcgcacgtcg tcgcccggga cgtactcgcg
    16561 cacctcggca aactcgatgc cgcgcccctt gaacacgctg tgatattcgc ccgcgaagat
    16621 gccgtcgacc aatcggcgcg tgcggatgtg gacctgtttt acctgcttgt agacttcggc
    16681 gggcagcatg ggcgcggacg ggtcgctcac ggcaccggca ggcgatcgaa aatctgctgt
    16741 accaggtcct cggtggtgat gtccgcggcc tcggcctcgt aggtgagcag catccggtgc
    16801 ctgagcacgt ccatgccgat cgacttgatg tcctgcggcg tcacgtagcc gcgcccgtga
    16861 atgaacgcat aggccttggc cgccagcgcc aggtagatcg agccgcgcgg cgaggcgccg
    16921 aagtcgatgt agcggccgat gtcgaggccg tgggcggcag gctcgcgcgt cgcgtggacc
    16981 agatcgacga tgtagtgctt gatcttgtcg tcgaggtaga tctcgtcaac cacgccgcgc
    17041 gcgcgcagga tcgctgcggc gtcggtcacc gccgccagcg gcggctcggg gctcgtggtc
    17101 gccatgcgct cgaggatctc cagctcctcg tcgcggctgg gataggtgat cttgagcttg
    17161 agcatgaacc ggtcgatctg ggcctcgggc agcggatagg tgccctcgag ctcgagcggg
    17221 ttctgcgtcg ccagcaccag gaacggctgg ggcaggcgga agctctcgtc gccgatggtg
    17281 acctggttct cctgcatcgc ctccaggagc gcgctctgca ccttggccgg cgcccggttg
    17341 atctcgtcgg cgagcaccag gttggcgaac accggcccct tgcgcaccgt gaacgccccc
    17401 gactggggct ggtagatcag ggtgccgacc aggtcggcgg gcagcaggtc gggggtgaac
    17461 tggatgcgct gataagcggc gtgcaggccc tcggcgagcg ccaccacggc gcgcgtcttg
    17521 gccagccccg gggcgccctc gatcagcacg tggccattgg cgagcaagcc gaccaggatc
    17581 cgctcgagca tgtatttctg gccgacgatc ttgcgctcca tggcccgcat caggtcgccg
    17641 acgaattgac tctgcgtctc gaccagggcg tctatctcgc tcacatccaa aggcatcacc
    17701 agccgctgtc ccgtgctcaa ggatggttga aggcgggcac taatagcaca ggcgcccgcg
    17761 atctcaatag catggcgggc gccgatcccc gcaccctact cggcagcctg ggcatacggc
    17821 cggccagtcg ccgcgagcac ctcgcgatag gcgcgctcga tgtcgcccgc gccggcgttg
    17881 gcggcctgat cgaggctctt ggagatggtc ggcgcggact cggccccgca cacccgctgc
    17941 accggctggt cgaggtagtc gaacaggcgg cgctggatct cgtcggcgac cgggccgccc
    18001 cagctggtgc cgcgggtgcc ctgctcgacg atcagcacgt tgttggtctt ggcgacgctg
    18061 ccgccgatgg tctgccagtc cagccccgcg cggtcgagcg agcgcaggtc gatgacctcg
    18121 gccgagacgc ccaggcggtc cgcgacctcg acgcagggac gcaccatcga cagcgaggcg
    18181 agcacggtga agtcctcgcc cgggcgcacc accttggcct tgcccagcgg gatgaagtag
    18241 tcgaggtcgt cgaccggcgc cgggccggtg gccccgtaga ggtcgagatg ctcgaccacc
    18301 agcaccgggt cgtcgcaggc gagcgcgctg ttcatcagcc cgacatagtc gaacggggtc
    18361 gacggcgcga cgatgcgcca gcccgggaac atcgcgtaca gcccgatcgg gtccatcgag
    18421 tgctgcgagc cgtagcccga gcccgccgcg accttgccgc gcagcaccag cggcaccgcc
    18481 atgtcgccgc cgaacatgtg gcgcatcttc ggaatctggt tgaacacctg gtcggcggcg
    18541 acgtagcaga agtcgggata catgaactcc accaccggcc ggaacttgcc gtcggcggcg
    18601 atgccgccgc cgaccccggc gaagccgccc tcggtgatcg gggtgccgat gatccggccc
    18661 gggaagcgct cggccaggcc gcgggtggcg ccgttggtgc cgccgcgcag gtggtggacg
    18721 tcctcgccga gcacgtagat gcgctcgtcg gtcgccattc gccggcccat caccgccgcg
    18781 accaggtcga cgaacttgcg ctcctcgacc gcgccggcaa acgtctcgag ctcgacgaaa
    18841 cgcgcgcccg cgagctcgga caggtcgccg cggatgccgt ggtcgacggt cgccggatcg
    18901 ggccacaagg cggggcggat gcgccggcgg ttgccctcgg gctcgagcag cgtctcgccc
    18961 gcgcgcgcca ccgatccttg cgcgtcggtc cgcagccgcg cgaggcggcg ctggtcgaga
    19021 tggccgagcc tgaccatctc ggcgcccagc cggtcgatcg cgtcgcgggc gcgccactcg
    19081 gcctcctcgt ccttgctgcg gtagccgaag gcgctgccgg gaagcggccc gttctggtgg
    19141 aagtagcggt agctgctggc ctcgacgatg gccggtccct tgccggcgcg gagcagcgcg
    19201 agcgcgcgca tcgtcgcgat gcgcactgcc agcgcatcca tgccgtcgca ggtgtaggcg
    19261 gggatgccgt aggccagccc gcgcgcggtc agccgccgct cgcgcgtcga ttccgcgatc
    19321 gtggtcgaga ccgcgtactg gttgttgtcg atgaagaaca ggatcggcag gtcccacagc
    19381 gcggccaggt tgaaggattc ggcgaccgcg ccctgatgga ccgcgccgtc gccgaagaag
    19441 gtgaccacca cgtcgccctc gcccgcctgc ttgcgcgacc aggcgacgcc ggtggcgatc
    19501 ggcacgccgc cgccgacgat ggcgttggtg ccgaccacgc ccgcgtcctt ccagcgcagg
    19561 tgcatcgagc cgccgcggcc gcggccgtag ccgggctcga ggccgagtat ctcggccaag
    19621 gtgcgcatca gcacggtctg catggcctcg ggcaaggcgc ccgcgaacgg gtcgggcttg
    19681 ccggtgtcga catagccgag cgccttggcc aggaaatggt ggtgggcgcg gtgggtgccg
    19741 ttgatctggt cggcggcgcg caacgccgag atgcaaccga ccgccgcccc ctcctggccg
    19801 atcgaggagt gcgccgggcc gtgcaccagc gcctcgccgg cgagctcgag caccagctcc
    19861 tcgaaggcgc ggatcaggtg caggtggtgg agcatgcgca gcagcgcgct ggcgtccgcc
    19921 ttgcgccagt cggcggccgt ggcgcggatc tcgagccact cggtgtcggt ggtcagcttg
    19981 cgcgcctgtg gcattgctcg tctcctcgac gcgtgttttc aataagttag ataaaatctt
    20041 tcaggttcat gtatcacatc agcatcactt cgtcccggcg tcctcggaca ccgacaggtc
    20101 catcgccatc cagtcgaggt ggctgcgccc cttggcgacc gaggcgagca ggcggtcgtg
    20161 gaccaggctc gcgagcggca tcggcacctc ggcggcggcg gcggcggcga gcaccaggtt
    20221 gacgtccttg aggccgagcg acagccggaa ccccggcggt tcgtactggc gcttggcgat
    20281 gccctcgccg tagatgcggt acaccgggca gtcgaacagc gagcccgaca agagctcgac
    20341 gaaggcggcg cggtcgacgc cgttcttctc ggccagcgcg caggcctccg agatcgcctc
    20401 gatcgccgcg ccgatcagga agttgcccga cagcttgacc acgttggccg ccccgggggc
    20461 gtcgccgaaa tcgcgcacgc cctggctgaa cgccccgagc agcggcgcca cgcggacctt
    20521 ggcggccttc tgtcccgaca gggcgaccca cagcttaccc gccgccgccg ccgccgggcg
    20581 gccgaacacc ggcgcgcaca ggtaggtggc gccggcctcg gcgtgcagcg cctccatgcg
    20641 ccgcgcggtg gcgaccgcga ccgtgctcat cgacacgtgc actccccccg gcccgagcgc
    20701 ggccagcacg ccgtcgggcc cgagcgcgat ctcctcgagc gcagcatcgt cggaaacgat
    20761 cgagagcacc acgccgccct tcgccgcgac gccgcccggc gcctcggccc gcttggcgcc
    20821 ggcctcgacc agcggcgccg ccttcgccgc cgtgcggttg tagacccgga ccgcgtggcc
    20881 ggcgtcgatc aggcgcctgg cgatcggcgc ccccatgttg ccgagcccga cgaaaccgac
    20941 cgcctccgcc atcgcctttc cctccggttg ctgacgcgca tgtcttgcgg gcgttataag
    21001 ggagcctgcg cccgcggtca cgcgatcggc gctgggcgag ccacggggag gacgcgatgg
    21061 ccggcaaggt attgggcacc aatcatacca gcttcacggt ctcggacctc gaccgcgcga
    21121 ttcagttcta cacccaggcg ctgggcttcc ggctgctgca ccggggcggg cgcgatgcgg
    21181 cgatgatcga gaaggtggtc ggcgtgccgg gcgcggacat cgaggtcgcc tacctgcagg
    21241 cccccgggca ccgcatggag ctgatccgct atctcggccc cgccgaccgc ggccgggtgg
    21301 tgagccgccc gtgcgatacc ggcttcgccc acgtcgcctt cgacgtcgac gacgtcgacg
    21361 cggtgctcgc cgccgccgcc cgccacgggg tgaagccggt cggcccggtc caggacctgc
    21421 accacggccc caacgccggc gggcgcgtgg tctacacccg cgaccccgac ggcatcacgg
    21481 tcgagttcat cgagcagccg aagggttagg cgaggccctt gcgccacagc gcggcctcgc
    21541 gctcgcggcg ctcacgcagg ccgcgcgagt cgggccacag gcggcgcatc gccaacagct
    21601 ggtcagggac ctgatcgacc ttaccgccct gcaacaggcc gcggatcgtg cgcatctcca
    21661 ggcggcggtc gctgccgggc tcgtcgtcga ggtcggtgcc gcggttgaac actaggctga
    21721 ccaggacgct gcggcacagg ccgggcaggt tggcgaacgc ggtatagacg ccgcgcgtgc
    21781 gcaccacctg gagcggcagg ctgcggccga tgaacacccg ccaggcggtg gtccacggga
    21841 cgcggagtcc cgacagggcc tcggccgccg cccggttgcc gagcttgccg agatggggcc
    21901 tgagcgccgc cagcacgtcg gcgggcagct tgtcgcccca gtcggtctcg aagatgtcgg
    21961 cggagaagcg caggtcgtag ccgacgccga tggtgatgcc gctctgctcg cccggccact
    22021 ggggaaaggt tgcctgcgcc tcgtagaagt cgcgcccgcc cacctcctcg ttggcgatga
    22081 aggtgacccc ttcggtgggc aggtcgggcg acggctcggc ctgtgccgcg agccagtcga
    22141 tcagcgcctg atcggcctcg gtcccacccg gtagcccgga cagcgcgcgc gcatcggcta
    22201 cggcgcgccg cgtgttgcgc ccgatctggc cgtcggtggc gagcaggccg ccgaccttgt
    22261 tgagcagccc ttgcagccgc tcgatctcgg ccttggtcat ggttacgaga cctcgaagtg
    22321 ggcgtcgacc tcgaccgcga tgttgaacgg cagcacgctg gtgccgaccg cgaagcgcgc
    22381 gtggcgcccg gcgtcgccga acacctcgac gaacaggtcg gaggcgccgt tgaccacctt
    22441 cggctgatcg aagaagtcgg gcgcggagtt gacgaagccg ccgaccttga cgcagcgcac
    22501 gacgcggtcg aggtcgccgt cgcacgccgc cttgagctgg gcgagaacgt tgagcgcgca
    22561 cagccgcgcc gccgcctgcc cctgctcgat cgtcaggtcg gcgccgaccc ggcccttata
    22621 tttgacctcg ccgtcgagga tcggcccctg cccggcgacg aacaccaggt ttccggtgcg
    22681 cacccagccg acatagttgg cgaccggcgc cgccgccgcg ggcagctcga cgccgagctc
    22741 ggcaaggcgc gcgtcgatcc gtcccgccgc caacgccgcg ccgagcggga aggcgcggcc
    22801 caacgccgcg cccgcaagcg ccgtcccggc aaggcgcagc accgcgcgcc gctcaagccc
    22861 aggtgcattc atctcgagtc cctccctcgc cgcggccgct acgtcaccag cgcgcgcacg
    22921 gcctcgacga tcttttcctg cgaggggatc gccgcgcgct cgagctcgtc gttgtagggc
    22981 atcggcgtgt ccagcgcgcc gaggcgcgcg atcggcgcat cgagatagtc gaacaccttc
    23041 tcgaccgcga gcgcggccac ctcggccccg aagccgccgg tgcgccaggc ctcgtgcacg
    23101 atcaacagcc ggttggtctt gcgcaccgag tcgaggatcg tcgcctcgtc gagcggcttg
    23161 agcgtgcgcg ggtcgatcac ctcgacctcg atgccctcgc gcgcgagc
//
LOCUS       DASWHU010000381         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3229352, whole genome shotgun sequence.
ACCESSION   DASWHU010000381 DASWHU010000000
VERSION     DASWHU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229352"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGB88_14170"
     CDS             <1..228
                     /locus_tag="VGB88_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648648.1"
                     /translation="GAGADESSRRAREGVQSGELRPLGQVLGQVTSAYPGRVIDVKLT
                     GSVYRVKVLTASGRVVVVSVDGRTGRIMGAR"
     gene            228..902
                     /locus_tag="VGB88_14175"
     CDS             228..902
                     /locus_tag="VGB88_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9648649.1"
                     /translation="MRVLVVEDDTTLAAQLRAALDQAGYAVDAAGDGEEAQFLGETEP
                     YDVVVLDLGLPVVDGVTVLERWRRAGHAVPVLILTARDSWHDKVAGFDAGADDYLAKP
                     FRMEELLARLRALIRRASGHASARLACGPIELDTARSQVTVEGAPVKLTAHEYKLLAY
                     LMHHQGQVVSRTELIEHIYDQSFDRDSNTIEVFVARLRKKLGVPAIETVRGLGYRLGV
                     ADADHA"
     gene            895..>2069
                     /locus_tag="VGB88_14180"
     CDS             895..>2069
                     /locus_tag="VGB88_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9648650.1"
                     /translation="MPEAPRGRSIAFRLLAGGILWIVLALGAGGLGLSFLFRGHVERV
                     FDARLEVLLETLIAAAEMSEDGTELAVGPLPGEPRFARPYSGWYWQVSGSGGIEVRSR
                     SLWDQALPLATPGGGTALTEVAGPGGERLRVLERDITLPGLDAPLRFAVAAETGELAR
                     ELRGFQATLWIALGALGAGLAAALIAQVRYGLRPLRAVRAALAGVREGRTARIAGALP
                     AEVGPLVDELNALLDHNADMLERMRRHVGNLAHALKGPLTVLTNAGAGETGSFADIVR
                     AQTDAMRRDVEHHGARARAAGAARLIGARTPLAPIIDRLKETLLLLHAERQVAIAVAA
                     EGAATFGGEQHDLEEMLGNLLDNACKWAAHQVRLSVRVERGRLELTVDDDGPGIELPA
                     "
BASE COUNT          251 a          752 c          788 g          278 t
ORIGIN      
        1 ggcgcgggcg ccgacgagtc ctcgcggcgc gcccgcgagg gcgtccagag cggcgagctg
       61 cgcccgctcg gccaggtgct ggggcaagtg acgagcgcct atccggggcg ggtgatcgac
      121 gtgaagctca ccggctcggt ctatcgggtc aaggtgctga ccgcctcggg gcgggtggtg
      181 gtggtcagcg tcgacggccg caccgggcgc atcatgggcg cgcgctgatg cgggtgctgg
      241 tggtcgagga cgacacgacg ctggccgcgc agctgcgcgc cgcgctcgac caggcgggct
      301 acgcggtcga cgccgccggc gacggcgagg aggcccagtt cctgggcgag accgagccct
      361 atgacgtggt cgtgctcgac ctcggcctgc cggtggtcga cggcgtcacc gtgctcgagc
      421 gctggcggcg cgccggccac gcggtgccgg tgctgatcct gaccgcgcgc gacagctggc
      481 acgacaaggt cgccggcttc gatgccggcg ccgacgacta cctggccaag ccgttccgga
      541 tggaggagct gctggcgcgc ctgcgcgccc tgatccggcg cgccagcggc catgccagcg
      601 cgcggcttgc ctgcggcccg atcgagctcg acaccgcgcg ctcgcaggtg acggtcgaag
      661 gcgcgccggt caagctcacc gcgcacgagt acaagttgct cgcctatctg atgcatcacc
      721 aaggacaggt ggtctcgcgc accgagttga tcgagcacat ctacgaccag agcttcgacc
      781 gcgattccaa caccatcgag gtgttcgtcg cccgcctgcg caagaagctg ggcgtgccgg
      841 cgatcgagac cgtgcgcggc ctcggctacc ggctgggggt cgccgacgcc gatcatgcct
      901 gaggcgccgc gcggccgctc gatcgccttc cgcctgctcg ccggcggcat cctgtggatc
      961 gtgctggcgc tcggcgccgg cgggctgggc ttgtcattcc tgttccgcgg ccatgtcgag
     1021 cgggtgttcg acgcccgcct cgaggtgctg ctggaaaccc tgatcgcggc cgccgagatg
     1081 tcggaagacg gcaccgagct cgcggtcggg ccgctgcccg gcgagccgcg cttcgcgcgt
     1141 ccctattccg gctggtactg gcaggtctcg ggctcgggcg ggatcgaggt gcgctcgcgc
     1201 tcgctgtggg accaggcgct gccgctggcc acgcccggcg gcggcaccgc gctcaccgag
     1261 gtggccggcc ccggcggcga gcggctgcgg gtgctcgagc gcgacatcac cttgcccggg
     1321 ctggacgcgc cgttgcgctt tgcggtcgcc gccgagaccg gcgagctcgc gcgcgagctg
     1381 cgcggcttcc aggcgacgct gtggatcgcg ctcggagcgc tcggcgccgg gctcgccgcg
     1441 gcgctcatcg cccaggtccg ctacgggctc cggccgctgc gcgcggtgcg cgccgcgctc
     1501 gccggggtgc gcgaggggcg cacggcgcga atcgccggcg cgctgccggc cgaggtcggg
     1561 ccgctggtcg acgagctcaa cgcgctgctc gaccacaacg ccgacatgct cgagcgcatg
     1621 cgccgccacg tcggcaacct cgcccatgcg ctcaagggcc cgctcaccgt gctgaccaac
     1681 gccggcgccg gcgagaccgg gtcgttcgcc gacatcgtgc gcgcccagac cgacgcgatg
     1741 cgccgcgacg tcgagcatca cggcgcgcgc gcgcgtgccg cgggcgcggc gcgcctgatc
     1801 ggcgcgcgca cgccgctcgc cccgatcatc gaccgcctca aggagacgct gctgctgctg
     1861 cacgccgagc gccaggtggc gatcgcggtc gctgccgagg gcgccgccac cttcggcggc
     1921 gagcagcacg acctcgagga gatgctcggc aacctgctcg acaacgcctg caagtgggcc
     1981 gcgcaccagg tgcggctgtc ggtccgggtg gagcgcggcc ggctcgagct gacggtcgac
     2041 gacgacgggc cggggatcga gctgccggc
//
LOCUS       DASWHU010000382         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1371431, whole genome shotgun sequence.
ACCESSION   DASWHU010000382 DASWHU010000000
VERSION     DASWHU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371431"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VGB88_14185"
     CDS             complement(<1..452)
                     /locus_tag="VGB88_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9648651.1"
                     /translation="MKFAGTESYVATDDLMVAVNAAITLERPLLVKGEPGTGKTMLAE
                     EIARALGKDLIRWHIKSTTTAQQGLYEYDAVARLRDGQLGDARVHDIANYIKRGKMWD
                     AFAAGAAPVLLIDEIDKADIEFPNDLLLELDRMEFFVYETGETVAARQR"
     gene            582..2102
                     /gene="iaaH"
                     /locus_tag="VGB88_14190"
     CDS             582..2102
                     /gene="iaaH"
                     /locus_tag="VGB88_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indoleacetamide hydrolase"
                     /protein_id="HEX9648652.1"
                     /translation="MPQGLPLTRRAFVAAAAASAFTLAAGGRTGARAALSDDAYATMT
                     AAQAIEAIGAGRTTVLDYVEALMARADRLKMLNAFITQDKDVVRAAARALDARIGAGE
                     SAGPLSGLPFCVKDNTNTSDMQTTGGTVAFKGWLPPANAPVVQSLRDAGGVMIGKTNM
                     HELAFGITSNNAAWGPVGNPYNAGLIPGGSSGGTAAAVAAGLAPVGLGSDTGGSCRIP
                     AALCGCVGFRPTLGRYPQDGIIPLSSTRDTSGPLARTVADAILVDSICAADAPMQLDL
                     SLSGARIGVPRQYFYDNLDRQLAAVVEHALDTLADAGAEPVEVDLPGIADLNGAVSFP
                     VVLYEVLREMSAYLYKNGSPLTVAEVVAKVAGAGERGILSSQLGAEAVPAAVYRDALV
                     VARPRLIAAYQAYFDDNRLAVMMVPTTPLPARPIGQEETVELNGEQVPTFLTYIRNTD
                     PPSNAGVPCLSVPAGLTADGLPVGVEFVGPPGADATVLGIGRAFEAARGPFPGPEL"
     gene            2125..>2845
                     /gene="panB"
                     /locus_tag="VGB88_14195"
     CDS             2125..>2845
                     /gene="panB"
                     /locus_tag="VGB88_14195"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9648653.1"
                     /translation="MSVAKPAGAPKTHRRVSVTDIVARKGGTTPIVCLTAYTALTAKL
                     LEDHVDLLLVGDSLGMVIYGLETTVGVSLEMMIAHGQAVVRGSTKPCIVVDLPFGSYQ
                     ESPQAAFRSSARVMAETGCNAVKLEGGVEMAATVRFLVERGVPVMGHVGLTPQHVHRF
                     GGFRPQARTEAEAEALVRDALAIAEAGAFAIVLEAVPEAAARRVTDSVAVPVIGIGAG
                     KAVDGQILVTEDIAGLFVDFKP"
BASE COUNT          387 a         1023 c         1029 g          406 t
ORIGIN      
        1 cgctggcgcg cggcgaccgt ctcgccggtc tcgtagacga agaactccat gcggtcgagc
       61 tcgagcaaga ggtcgttggg gaactcgatg tccgccttgt cgatctcgtc gatcagcagc
      121 accggcgcgg caccggcggc gaacgcgtcc cacatcttgc cgcgcttgat gtagttggcg
      181 atgtcgtgga cgcgcgcatc gccgagctgg ccgtcgcgca ggcgcgccac cgcgtcgtac
      241 tcgtacagcc cctgctgggc ggtggtggtg gacttgatgt gccagcggat caggtccttg
      301 ccgagcgcgc gggcgatctc ctcggcaagc atggtcttgc cggtgccggg ctcgcccttg
      361 accaagagcg ggcgctcgag cgtgatcgcg gcgttgaccg cgaccatcag gtcgtcggtg
      421 gcgacatagg attcggttcc ggcgaacttc atcgagtcct cttcggaatt tggcgaagcc
      481 gaggcacctt cggccaacgc cccggcgccg tcaacccggc tcgcccggga gcgttcgggc
      541 tgctatagtc gcaccaatat cggggatcag ggggaaggac catgccacag ggactgccac
      601 tcacccgccg cgccttcgtc gccgccgcgg cggcgagcgc gttcaccctc gccgcgggcg
      661 ggcgcaccgg cgcgcgcgcg gcgctgtccg acgacgccta cgcgaccatg accgcggccc
      721 aggcgatcga ggcgatcggc gccgggcgga ccaccgtgct cgactatgtc gaggcgttga
      781 tggcgcgcgc cgaccggctc aagatgctca acgccttcat cacccaggac aaggacgtgg
      841 tgcgcgccgc cgcgcgggcg ctcgatgcgc gcatcggcgc cggcgagagc gcgggaccgc
      901 tgtcgggcct gccgttctgc gtcaaggaca acaccaacac cagcgacatg cagaccaccg
      961 gcggcacggt cgcgttcaag ggctggctgc cgccggcgaa cgcgccggtg gtccagagcc
     1021 tgcgcgacgc cggcggggtg atgatcggca agaccaacat gcacgagctc gccttcggca
     1081 tcaccagcaa caacgccgcc tgggggccgg tcggcaaccc gtacaacgcc ggcctgatcc
     1141 cgggcggctc cagcggcggc accgccgcgg cggtcgccgc cggcctggcg ccggtcggcc
     1201 tcggctctga taccggcggc tcgtgccgga ttccggcggc gctgtgcggc tgcgtcggct
     1261 tccgcccgac cctcggccgc tatccgcagg acggcatcat cccgctgtcg agcacgcgcg
     1321 atacctccgg ccccctggcg cgcaccgtcg ccgacgcgat cctggtcgac tcgatctgcg
     1381 ccgccgatgc cccgatgcag ctcgacctgt cgctgtccgg cgcgcgcatc ggcgtgccgc
     1441 gccagtactt ctacgacaac ctggaccgcc agctcgccgc ggtggtcgag cacgccctcg
     1501 acacgctcgc cgatgccggc gccgagccgg tcgaggtcga cctccccggc atcgccgacc
     1561 tcaacggcgc ggtcagcttc ccggtggtgc tgtacgaggt gctgcgcgag atgagcgcgt
     1621 atttgtacaa gaacggcagc ccgctcacgg tcgcggaggt ggtggccaag gtggcgggcg
     1681 cgggcgagcg cggcatcctc agctctcagc tcggcgccga ggcggttccg gccgcggtct
     1741 accgcgacgc gctggtggtc gccaggccgc gcctgatcgc cgcctaccag gcctatttcg
     1801 acgacaaccg gctggcggtg atgatggtgc cgaccacgcc cctgccggcg cgcccgatcg
     1861 gccaggagga gacggtcgag ctcaacggcg agcaggtgcc gacgttcctc acctacatcc
     1921 gcaacaccga cccgccgagc aatgccggcg tgccgtgcct gagcgtgccg gcggggctga
     1981 ccgccgacgg gctgccggtc ggggtcgagt tcgtcggccc gccgggcgcg gacgcgaccg
     2041 tgctcggcat cggccgcgcc ttcgaggccg cgcgcggccc gttccccggg cccgagctgt
     2101 agccggcggg gagcggagcg ggcggtgtcg gtcgccaagc ccgcgggcgc gcccaagacc
     2161 caccggcggg tctcggtcac cgacatcgtc gcgcgcaagg gcggcacgac gccgatcgtg
     2221 tgcctgaccg cctatacggc gctgaccgcc aagctgctcg aggaccacgt cgatctgctg
     2281 ctggtcggcg actcgctcgg catggtgatc tacggcctgg agaccacggt cggggtcagc
     2341 ctcgagatga tgatcgccca tggccaggcg gtggtgcgcg gctcgaccaa gccgtgcatc
     2401 gtggtcgacc tgccgttcgg ctcctaccag gaatcgcccc aggccgcgtt ccgctcgtcc
     2461 gcgcgggtga tggccgagac cggctgcaac gcggtcaagc tcgagggcgg ggtggagatg
     2521 gcggcgaccg tgcgcttcct ggtcgagcgc ggcgtgccgg tgatgggcca tgtcgggctg
     2581 acgccgcagc acgtccaccg cttcggcggc ttccgcccgc aggcgcgcac cgaggccgag
     2641 gccgaggcgc tggtgcgcga cgcgctggcg atcgcggagg cgggcgcgtt cgcgatcgtg
     2701 ctcgaggcgg tgcccgaggc ggcggccagg cgcgtcaccg acagcgtcgc ggtgccggtg
     2761 atcggcatcg gcgccggcaa ggcggtcgac ggccagatcc tggtcaccga ggacatcgcc
     2821 ggcctgttcg tcgacttcaa gccca
//
LOCUS       DASWHU010000383         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2136680, whole genome shotgun sequence.
ACCESSION   DASWHU010000383 DASWHU010000000
VERSION     DASWHU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136680"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VGB88_14200"
     CDS             <1..993
                     /locus_tag="VGB88_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX9648654.1"
                     /translation="FTAPLAGETVIDDAVQGRVTLDNRQLDDMVLLRADGTPTYMLSV
                     VVDDHDTAISHVIRGDDHLTNAFRQTQLYRALGWQPPAFAHIPLIHGADGAKLSKRHG
                     ALGVEAYRDLGFLPEAMRNYLLRLGWAHGDDEVISTEQAIAWFDLDGIGRSPARFDLA
                     KLESLNGIYIRAAADERLVELILPRLAAALGGPLDDRARARLQAGMAGLKPRAKTLVE
                     LAEAALIYVREPPLEMTEKARRLLDGEARARLARLAEALAALDDWSQAGVEAAVRALA
                     DAEQVKLGALAQPLRAALTGRTESPGIFEVMAVLGRDQVRARLAAVSRATGTTG"
     gene            1164..2447
                     /gene="gltA"
                     /locus_tag="VGB88_14205"
     CDS             1164..2447
                     /gene="gltA"
                     /locus_tag="VGB88_14205"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX9648655.1"
                     /translation="MTLTDSRPGGRAVELPVLSGTMGPDVIDIRRLYAETGMFTFDPG
                     YTSTGSAQSTITFIDGEAGMLLHRGYPIEELAERSDFMEVAYLLLYGALPTRAERARF
                     VHNVVYHTMLHEQLIYLYRGFRRDAHPMAVLIGVVGAMSAFYHDSIDVNDPRQREIAS
                     HRLIAKMPTIAAMAYKYSIGQPFVYPRNELGYAENFLHMLFAVPAEDYKVNPVLARAI
                     DRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIASLWGPAHGGANEAVLRMLGE
                     IGSADRIPEYLKRAKDKDDPFRLMGFGHRVYKNYDPRAAVMRTSAHEVLAELGHENDP
                     LLDLAMKLEKIALEDDYFVERKLFPNVDFYSGIILRAMGIPSELFTVIFAVARTVGWV
                     AQWNEMIEDPGQKIGRPRQLYVGPTKRHYVPIDKR"
BASE COUNT          417 a          956 c          879 g          402 t
ORIGIN      
        1 ttcacggcgc cgctcgccgg cgagaccgtg atcgacgatg cggtccaggg ccgggtcacc
       61 ctcgacaacc gccagctcga cgacatggtg ctgctgcgcg ccgacggcac gccgacctac
      121 atgctgtcgg tggtggtcga cgaccacgac acggcgatca gccacgtcat ccgcggcgac
      181 gatcacctga ccaacgcctt ccgccagact cagctgtacc gcgcgctcgg ctggcagccg
      241 cccgccttcg cccacattcc gctgatccac ggcgccgacg gcgccaagct gtccaagcgc
      301 cacggcgcgc tcggcgtcga ggcgtatcgc gacctcggct tcctgcccga agcgatgcgc
      361 aactatttgc tcaggctcgg ctgggcgcac ggcgacgacg aggtgatctc gaccgagcag
      421 gcgatcgcct ggttcgatct cgacggcatc ggccgctcgc cggcgcggtt cgacctcgcc
      481 aagctggaga gcctgaacgg catctacatc cgcgcggccg ccgacgagcg cctggtcgag
      541 ctgatcctgc cgcggctcgc cgccgccctc ggcggtccgc tcgacgatcg cgcccgcgcg
      601 cgcctgcaag ccggcatggc cggtctcaag ccgcgcgcca agacgctggt cgagcttgcc
      661 gaggcggcgc tgatctacgt gcgcgagccg ccgctcgaga tgaccgagaa ggcccggcgc
      721 ctgctcgatg gcgaagcgcg cgcgcgtctc gcccgcctgg ccgaggcgct cgccgcgctc
      781 gacgactggt cgcaggccgg ggtcgaggcc gcggtgcgcg ctttggccga cgccgagcag
      841 gtcaagctcg gcgccctcgc tcagccgttg cgcgcggcgt tgaccgggcg caccgagtcg
      901 cccggcatct tcgaggtcat ggcggtgctc ggccgcgacc aggtgcgggc gcggctggcg
      961 gcggtctcgc gggccaccgg cacgaccggt tgagtctcaa caccgaaggc gtgcgctggc
     1021 tcgggcgaaa ttgccaaggc ggggcggact cactatgctc ccccgcccgg gatgcggcgc
     1081 cacgcgcacc cggcaaatct ggaaaggtga tgggaatggc caagacggct gggaggaccg
     1141 cgaccgaagc gcctgcgcgg accatgacct tgaccgactc gcggccgggc gggcgcgcgg
     1201 tcgagctgcc ggtgctgtcg gggaccatgg gcccggacgt gatcgacatc cgccgcctgt
     1261 acgccgagac cggcatgttc acgttcgatc cgggctatac ctcgaccggc agcgcccagt
     1321 cgaccatcac cttcatcgac ggcgaggccg ggatgctgct gcatcgcggc tacccgatcg
     1381 aggagctggc cgagcggtcg gacttcatgg aggtcgccta cctgctgctc tacggcgcgc
     1441 tgccgacccg ggccgagcgc gcgcgcttcg tccacaacgt cgtctaccac accatgcttc
     1501 acgagcagct gatctatctg tatcgcggtt tccgccgcga cgcgcacccg atggcggtgc
     1561 tgatcggcgt ggtcggcgcg atgtcggcgt tctatcacga ctcgatcgac gtcaacgatc
     1621 cgcgccagcg cgagatcgcc tcgcaccggc tgatcgccaa gatgccgacc atcgccgcga
     1681 tggcgtacaa gtattcgatc ggccagccgt tcgtctatcc gcgcaacgag ctcggctacg
     1741 cggagaactt cctgcacatg ctgttcgcgg tgccggccga ggactacaag gtcaaccctg
     1801 tgcttgcccg cgccatcgac cgcatcctga tcctgcatgc cgaccacgag cagaacgcct
     1861 cgacctcgac cgtgcgcctc gccggctcgt cgggcgccaa tccgttcgcc tgcatcgccg
     1921 ccggcatcgc ctcgctgtgg ggaccggcgc atggcggggc gaacgaggcc gtgctgcgca
     1981 tgctcggcga gatcggcagc gccgaccgca tccccgaata cctcaagcgt gccaaggaca
     2041 aggacgatcc gttccggctg atgggcttcg gccaccgggt gtacaagaac tacgacccgc
     2101 gcgcggcggt gatgcgcacc tcggcgcacg aggttctcgc cgagctcggc cacgagaacg
     2161 acccgttgct cgacctggcc atgaagctgg aaaagatcgc gctcgaggac gactacttcg
     2221 tcgagcgcaa gctgttcccg aacgtcgact tctactcggg catcatcctg cgcgcgatgg
     2281 gcataccgag cgagctgttc acggtcatct tcgcggttgc gcggaccgtc ggctgggtcg
     2341 cgcagtggaa cgagatgatc gaggatccgg gccagaagat cggccgcccc cgccagctct
     2401 acgtcggccc gacgaagcgc cactacgtgc cgatcgacaa gcgctgaggc cgcgcgccaa
     2461 aggtcccggc ggacccgcgg ctcctcgtca ttccggcgaa cgagcctatg aagcaatcgc
     2521 cgtacgcgcc cattatcgtc attcccgcga aagcgggaat ccagactttt caatgggtta
     2581 ctggaccccc gcttgcgcgg gggtgacgga ccagggccat ggccgcagac ttgcgcccca
     2641 cctcgccttc gccg
//
LOCUS       DASWHU010000384        31004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1767282, whole genome shotgun sequence.
ACCESSION   DASWHU010000384 DASWHU010000000
VERSION     DASWHU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 31004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31004
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767282"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1258
                     /locus_tag="VGB88_14210"
     CDS             380..1258
                     /locus_tag="VGB88_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648656.1"
                     /translation="MTLDSGQALRRELLLKLLEVCEQPEVALAAARRMEAFILGRPSA
                     GPDAQEAASAEEGAGVEGDAYLARVLGPVTEKTDQPSLRAPSTGWPGHTAAPSTASPI
                     SRRRWIDRFDRLLREQWDRGVPVAEIAARLDRTTASINGRVRVLGLAKRRGNTAQGKG
                     TRPGKNDARNSGGGLSGGGRKAAGAKAPRPRSCAPDTPDRPAKAAEGKLIAEFIAMHG
                     VRREPVTIDSVVKFLQSRGHCVVRSGQGRFELDNREHLSARELLVRANIAKQRMRQPK
                     FPDSVLRSAGSAEEVA"
     gene            complement(1321..2685)
                     /locus_tag="VGB88_14215"
     CDS             complement(1321..2685)
                     /locus_tag="VGB88_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9648657.1"
                     /translation="MVLALCAALMLGFQVAFTLSGVALVFALVGAAFDIFELAFVAAL
                     PNRIYGIMTNDLLIAVPLFVFMGVMLERSKLAEALLDTMALLLIGVRGGLGIAVSAVG
                     ALLAASTGIVGATVVTMGLLSLPTMLRRGYGAPIACGSICAAGTLGQIIPPSIVLILL
                     GDQISAAYQQAQLSAGNFAPEPVSVADLFVGALIPGFTLVALYIVWQFIYANIYPHRM
                     PSVPAAERAAISRGEMAGRVVTVLLPPLLLIVLVLGSILVGVATPTEAAGVGAMGSLG
                     LAAARRQLTRNNLRDVMRSTAKVSSMVFVILIGAQLFSLVFRGFHGDEIVGDFLRGLP
                     GGVFTAMLLTMALMFVLGFFLDFIEIVFVVVPIIGPAILMNEGISPVWFAIMIAVNIQ
                     TSFLTPPFGFALFYLRGVAPPEVKTTDIYRGVVPFVAMQLLTLVILAAFPAMATWLPS
                     LIYS"
     gene            complement(2820..3362)
                     /locus_tag="VGB88_14220"
     CDS             complement(2820..3362)
                     /locus_tag="VGB88_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9648658.1"
                     /translation="MRALLGVARAIDALNEHMGRLFAWLTVVMVLVTVMIVVLRYGFN
                     LGFIWMQESVRFMHAFVFLLCAGYTLLHDEHVRVDVLYVKMSERSKAKVDIFGNIVFL
                     IPVCAVIVYFSWNYVLNSWAENEGSLEERGLHAVYLLKTTIWVFAGTMILQAVSRIIH
                     ALAMLRGVEHEVAEPHEHAL"
     gene            3688..4041
                     /locus_tag="VGB88_14225"
     CDS             3688..4041
                     /locus_tag="VGB88_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648659.1"
                     /translation="MKTSSPTFLRSTIPALALALAIGSTAATGAHADGVRTNHAPQVA
                     RGYVRHADEVTAFGLSDHAAIAVAAPRSGRGYVRRADEVAAFGLSDRKPIVVAAPRVG
                     RGYLPPFETGLAAGR"
     gene            4257..4946
                     /locus_tag="VGB88_14230"
     CDS             4257..4946
                     /locus_tag="VGB88_14230"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX9648660.1"
                     /translation="MLHAAGIFYAERGFDRVSTREVTRAAGANLAAIAYYFGGKSGLR
                     EAVLDHVVMASRERIAGIFERLSKAVEESGGAPARLAEATYRFADEFLHATLPLSRET
                     WWVTVITRAMGNLSAGEERIYEAVFRPVNRTVQKLVAAATGESDHDRLGVLTEAVVGD
                     FLTFCKNSSLVLRSLGWDGYSPERIDRIVPVVARRMLGRLGLPLPAQLAPLRAGAPAA
                     GEFRARAVGSA"
     gene            complement(4960..7182)
                     /gene="parC"
                     /locus_tag="VGB88_14235"
     CDS             complement(4960..7182)
                     /gene="parC"
                     /locus_tag="VGB88_14235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX9648661.1"
                     /translation="MTRESQAAQVIPVAFADALSERYLSYALSTIMSRSLPDVRDGLK
                     PVHRRLLYAMRQLRLDPASGYKKCARVVGDVIGKYHPHGEVAVYDAMVRLAQDFAVRY
                     PLVDGQGNFGNVDGDGAAAMRYTEARLTEVAEALLAGIDQDTVDFRATYDGEDSEPVV
                     LPAAFPNLLANGASGIAVGMATSIPPHNADELCQALLHLIKTPNACIETLVEMVPGPD
                     FPTGGVLAEPRAAVIEAYRTGRGAFRVRARWGIEKLGHGQYQVVVTELPYQVQKARLV
                     EKIAELMAAKRLPWLADVIDESAEDVRLVLEPRARTVEPAALMETLFRLTELETRVPL
                     NLNVLDATNTPRVMSLSEALQAFLDHRREVLLRRSRHRLERIERRLEVLDGLLVAYLN
                     LDEVIRIIREDDEPKATMMARWTLSDVQAEAILNMRLRNLRRLEEIEIRSEREALTGE
                     RKQVKALLRDERAQWRMVADEVRDIRRRFGKDTALGARRTEIGDAPAAEVVSLEGMVE
                     REPVTVICSEKGWIRAVRGHLAEDAEIKFKEGDRPWRRLHAQSTDKVLAFATNGRFYT
                     LACDRLPGGRGFGEPLRLMIDLANEHDVVALLVHRPQRRLLVAASDGRGFVVEETDVV
                     AQTRGGRQVLNVRGAVEAAVCVAAEGDRVAVVGDNHKLVVFGLDEVPVLARGRGVILQ
                     KYKDGGLSDARVFRAADGLGWRQGSRTRTLEDVADWQGKRGNAGRLAPKGFPSTNKFD
                     "
     gene            complement(7211..7960)
                     /gene="recO"
                     /locus_tag="VGB88_14240"
     CDS             complement(7211..7960)
                     /gene="recO"
                     /locus_tag="VGB88_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9648662.1"
                     /translation="MEWIDEAVVLSRRRHGETALIVHLLTRDHGRHAGLVPGGAGRRG
                     GSLWQPGNHVSCRWRARLAEHLGTVSGEPLRAYAALLLDDPLRLAAAASAAALVDAAL
                     AEREPHPALFHALCALLAALASDAEPGDWGAAYVGWEVGLLRELGFGLDLSACAVTGR
                     PDDLAWVSPRTGRAVSAEAGTPYGDRLLRLPAFLTAGRGTGEIADILAGLALTGHFLS
                     RHGIARAGSGLPVARDRLVTGFQRLSTTCGG"
     gene            complement(7977..8909)
                     /gene="era"
                     /locus_tag="VGB88_14245"
     CDS             complement(7977..8909)
                     /gene="era"
                     /locus_tag="VGB88_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9648663.1"
                     /translation="MAELAREVGTRCGYVALLGAPNAGKSTLLNRLVGAKVSIVTPKV
                     QTTRARVLGIASAGDTQIVFIDTPGIIAPRKRLERAMVQAAWAGARDADVVVVLVDAR
                     GGIGADSRRLLDRLAAEGRQAVAAINKVDAVKRPGLLALAAALDRETLADGRKLFTDV
                     FMISALTGDGVADLLHVLARRMPAGPWLYPEDQAADMPLRLLAAEITREQLFLQLRHE
                     LPYEIAVETEAWSERADGSVRIDQVIYVRRDSQRMIVLGKGGRQIKAVGSAARAGLEA
                     MLGQRVHLFLHVKVREDWPEDPERYRDLGLDYDV"
     gene            complement(8902..9588)
                     /gene="rnc"
                     /locus_tag="VGB88_14250"
     CDS             complement(8902..9588)
                     /gene="rnc"
                     /locus_tag="VGB88_14250"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9648664.1"
                     /translation="MPGSALAGAAAALGVEFRDPGLLEQALTHPSVGPGRVNNQRLEF
                     LGDRVLGLVIAARLYRGYADEDEGALARRFAPLVSAAGLAAVAARLDLGRFLVLGPGE
                     EASGGRTNPANLADALEAVIGALYVDGGLDAARAFVERHWRELIVRQVTPPKDAKTAL
                     QEWSQARGLGLPSYRLVASEGPDHRPLFRVAVTVAGHGAAEGEGAGKREAERAAAASM
                     LRRLAAGADG"
     gene            complement(9597..10358)
                     /gene="lepB"
                     /locus_tag="VGB88_14255"
     CDS             complement(9597..10358)
                     /gene="lepB"
                     /locus_tag="VGB88_14255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9648665.1"
                     /translation="MTQRSGRIAKKSRADGFWETFKTVVYAVLIATVVRTFAYEPFHI
                     PSGSMIPSLLIGDYLFVSKFSYGYSRYSFPFDIGVIEGRIMAHQPERGDIAVFKLPRD
                     NKTDYIKRLIGLPGDHIQVRDGLLYINDQPVQRRQIEDYELVDRIGNVTAVDQYVETL
                     PNGIGYRIIDSNPYGPLDNTDVYVVPADHYFAMGDNRDGSSDSRILSNVGYVPAENLV
                     GRAEILFFSTDGTAHFWEVWKWPFATRFWRFGILL"
     gene            complement(10471..10884)
                     /gene="acpS"
                     /locus_tag="VGB88_14260"
     CDS             complement(10471..10884)
                     /gene="acpS"
                     /locus_tag="VGB88_14260"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9648666.1"
                     /translation="MILGIGHDVCDASRIASALERFGDRFTHRVFTPVERERCERRLR
                     RADSYARRFAAKEACSKALGTGFRNGVHWADIGVVNQPGGKPTIELTGGALARLREIT
                     PAGMEARLDVSLTDDRELAHAIVIISVVPAAPSGA"
     gene            complement(10922..11677)
                     /locus_tag="VGB88_14265"
     CDS             complement(10922..11677)
                     /locus_tag="VGB88_14265"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9648667.1"
                     /translation="MTHDLRLGVNIDHVATIRNARGGRHPDPVRAARLAAEAGADGIT
                     AHLREDRRHISDTDIHRLKDEIDLPLNLEMAATAEMLDIALATRPPKVCLVPEKRQEL
                     TTEGGLDAAALHAALEPYIGRLHGAGIRVSLFVDPDRVQIAVAEALGADVVELHTGPY
                     CDAEGGRREAELDRLKAAAREVLARGLECHAGHGLSFDTVGPIAAMVEVSELNIGHFL
                     VGEAIFGGLESSIRRMRAIMDKARAEASGKRSA"
     gene            complement(11674..12315)
                     /locus_tag="VGB88_14270"
     CDS             complement(11674..12315)
                     /locus_tag="VGB88_14270"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX9648668.1"
                     /translation="MLGRRIRPHWVDRLWQLIWPRLGWRRASTYYGHRVRRLRGSPYK
                     VAAGFACGAAVSFTPLIGFHFALAALIAWLIGGNVIASAIGTAIGNPWTFPVIWLASY
                     RLGAWLLGFEHLVPEVAPEGMSISYIFDNPEAVFLPMMTGGTLLALAAWAISFWLVRR
                     AVARYQHMRAARIARKAERRAARRAAREAKAALAGHAAAPEARELAGGEIRER"
     gene            complement(12374..14506)
                     /locus_tag="VGB88_14275"
     CDS             complement(12374..14506)
                     /locus_tag="VGB88_14275"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9648669.1"
                     /translation="MVERVAAYDPNADEQALNRAYVFAMSRHGAQRRASGDPYFSHPL
                     EVAGILVDLKLDAASIITALLHDTVEDGVATEAEIAAQFGPEIARLVDGVTKLSKIEL
                     QSRSERQAENFRKFLLAMSEDLRVLLVKLADRLHNMRTLSHIEDADKRRRIALETMEI
                     YAPLAERIGMQRLKDELEDLSFAELYPEARESLVKRLDFLRETEGQLIPVIADELKQT
                     LAAAGIEAEVMGREKRPYSIWRKMERKHVPFEQIADVIAFRVVVADAAACYRALGAVH
                     QAYPTIPGRFKDYVSTPKPNGYRSIHTSVIGPQRRRVEIQIRSREMNEVAEYGVATHW
                     LYKQDADGRLMRDGRQYRWLRHLLDILEHASNPEEFLEHTKLEMFADQVFCFSPKGDL
                     ITLPAGSTPVDFAYAVHSDVGDTCVGAKINGRLAPLRTRLQNGDQVDILRSSVPAPDP
                     NWENFVATGKARARIRRFVRQRRNDEYASLGRSILEKTFLNADKLFGDQVLAPALAKL
                     AKRNPGDLFAAVGRGDINARHVLAAVHPDQAAKPGKVVPFVADRGRLDDRRRGAVPIK
                     GLTPGMAVHMSDCCHPLPGDRIVGLVTEGKGVTIHTIDCEVLESFIDSPERWLDVSWD
                     TSDVKPSVHTGRIVTVINNVPGSLNALTEAIARRHGNISNLKIVNRSPDFFEFVVDIE
                     VENLRHLTDIITTLRSHPSIYRAERAQG"
     gene            complement(14635..15018)
                     /gene="rpoZ"
                     /locus_tag="VGB88_14280"
     CDS             complement(14635..15018)
                     /gene="rpoZ"
                     /locus_tag="VGB88_14280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9648670.1"
                     /translation="MARVTVEDCVLRIPNRFELVMLAAQRARDISAGGSLTVERDNDK
                     NPVVALREIAEETVRTEALQEALVRGLQRHAEIDEPEEEAAAMDEGDLLSELEAMGGE
                     LPGMQIVETGGGQDKAAVESSDDDD"
     gene            complement(15099..15728)
                     /gene="folK"
                     /locus_tag="VGB88_14285"
     CDS             complement(15099..15728)
                     /gene="folK"
                     /locus_tag="VGB88_14285"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9648671.1"
                     /translation="MTDKSPSDHRVRRGDGPILIGLGANLPSERFGGPRQTLEAALAE
                     LERRGVRVVRRSRWYESAPVPRSDQPWYVNGVAAVETGLGPEALLAVLHAIEHDFGRV
                     RGARNAARAVDLDLLAYGDLVRAGPAAPLLPHPRVPERAFVLLPLAEIAPGWRLPGSG
                     ETVAALAARLPPDQEIRPLGVGEPEAGDRTAPVEPRKGRPSAQKPAGRH"
     gene            15888..16505
                     /locus_tag="VGB88_14290"
     CDS             15888..16505
                     /locus_tag="VGB88_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9648672.1"
                     /translation="MKFHPQERVAIFIDGANLYAAARALGFDIDYKRLLDTFTGACHL
                     IRAFYYTALVEDQEYSPIRPLVDWLDYNGYTMVTKPTKEFTDSSGRRKIKGNMDIELA
                     IDVMEMAEHVDHIVLFSGDGDFRRLIEAVQRKGVRASVVSTVRTQPPMVADELRRQAD
                     NFIELEDLSEHIARDPMARMARRAQEAADIEADEDVEEELDLGRA"
     gene            16555..17169
                     /locus_tag="VGB88_14295"
     CDS             16555..17169
                     /locus_tag="VGB88_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9648673.1"
                     /translation="MVAFRAANRARFAGYHNAPVDSFGPAAARLLVVGLAPGLRGANR
                     TGRPFTGDAAGELLYPTLIAHGFARGTYRARPDDRLELVSCRITNAVRCVPPNNKPTG
                     AELRACRGFLAAEIAAMPELRAVLALGRIAHDAVLAALGLGRASTPFAHGARHALASG
                     QALFDSYHCSRLNLNTGRLTPAMFDAVIARVRADLALLRQKVSL"
     gene            complement(17184..17741)
                     /locus_tag="VGB88_14300"
     CDS             complement(17184..17741)
                     /locus_tag="VGB88_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9648674.1"
                     /translation="MLGMIGKLAGGAALAVALAAATPGLAMTAHDFDFTSIEGKRLPM
                     SRFAGKVVLLVNTASFCGFTPQYEGLEALWRRYRDRGLVVLGVPSNDFGNQEPKDEGE
                     IKEFCEANYRVDFPMTAKQTVVGAHAHPLYRWIADSLGEAAEPRWNFHKYLIDKSGAV
                     TEAWPSQVKPESGTVTDAIESALEK"
     gene            complement(17772..18242)
                     /gene="smpB"
                     /locus_tag="VGB88_14305"
     CDS             complement(17772..18242)
                     /gene="smpB"
                     /locus_tag="VGB88_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9648675.1"
                     /translation="MAGGDLKQRIVAQNRKARHNYIIEDTVEAGLVLVGTEVKSLREG
                     RASLGDAFAERRDGEMVLVNSHIAHYPPANRLNHDPLRPRKLLLHRREIERLRGLVER
                     RGYTLVPLKLYFDERGRAKVELGIARGRKLHDKREREKELGWQRDKARLMRARS"
     gene            complement(18224..19129)
                     /gene="dapA"
                     /locus_tag="VGB88_14310"
     CDS             complement(18224..19129)
                     /gene="dapA"
                     /locus_tag="VGB88_14310"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9648676.1"
                     /translation="MFKGSITALLTPFEDGKVADKTFQAFVDWQVTQGAHGVVPCGTT
                     GESPTLSHTEHRRVTEVCIETVAKRVPVIAGTGSNSTEEAVSLTRHAYQAGADAALVV
                     TPYYNKPTQEGMYRHFEAVAAAADIPIIVYNIPGRSVVNMSVDTLARLARIPNILGVK
                     DATCDLTRPLLERQAIEGEFSFLSGEDGTALAYLANGGNGCISVTANVAPALCVEVQE
                     TWAKGDAAEALALHDRLMPLHTHLFAETSPAPAKYAASLLGLCSGELRLPMVEVGEAT
                     KAKVRAALAAAGLLSENVRQWRAAT"
     gene            19356..21332
                     /locus_tag="VGB88_14315"
     CDS             19356..21332
                     /locus_tag="VGB88_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9648677.1"
                     /translation="MAATAPDAAPAGKTPRVLSDAGLAHARSALEHVGHKRWREAQYD
                     AGRAGEPLVAKLVEWRRLADDGSGASFTTLAAFIDANPRWPGLATLRRNAEKAMPGTM
                     ADRDVVAWFERHPPASPTGKRRFAEALLAAGDAGHGLALIRDAWVNGDFDSRQEKAFL
                     AGHAKRLRPEDHVARLDRLLWDARVREAERQMPRVDDAHRRLALARIRLMAQAGGVDP
                     AVARVPPALAQDAGLIYERLRWRRRAGFDDGARELLDAQPADPVRAGRWWTERHILAR
                     RALDTGAITAAYRAAAHHGQAGPAEIAEAEWLAGWIALRFLDEPLIAYQHFVDMHQVV
                     AMPISIARAAYWAGRAADAAGVPGEAERWYRRAATQPTAFYGQLALLALGADELTLPA
                     DPVPGPNVVAAFERDELVRLARILGELGEADLMSRVIARIIDDADSPALGDLAARLGL
                     DYGLEPVAIRASKYAQRQGVATIAPAYPVPPAVASHLQSDSGRLEPGLMLAVARQESE
                     MNPNVVSSAGARGLMQLMPPTAAMVAKRLGLPYDAARLNGDPRYSLRLGGDYLAGLID
                     DFSGATVLAVTGYNAGPSRVRQWMRQNGDPRDADVDVIDWIELIPFNETRNYVQRVLE
                     GTQVYRVVLSEQEGRARLRLGDDIGVGPGRLAGR"
     gene            21416..22414
                     /locus_tag="VGB88_14320"
     CDS             21416..22414
                     /locus_tag="VGB88_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX9648678.1"
                     /translation="MRNGLPDIEDVVDAARRLRGKAVVTPLLRAPALDRIAGGTLLIK
                     AEPLQRTGSFKFRGAYNRISRIPEEERSRGVVSWSSGNHAQGVAAAAGLLGIPATIVM
                     PSDAPAPKLDNTRALGAEVVLYDRYAESREDIGTRIAEQRGATIIKPYDDPWIIAGQG
                     TVGLEAAGQALAAGLSPDHALVACSGGGLVSGTAIALTDHLPGITVHSVEPEGFDDTA
                     RSLAAGARLANDPAARSICDALLAPTPGELTFEINRRLLGPGIVVSDADARAAMRAAF
                     RHLRLVAEPGGALPLAAALSGRFDCRGKCVLVVVSGGNVAPELFAETLLAGEDPGG"
     gene            22407..23906
                     /gene="glpK"
                     /locus_tag="VGB88_14325"
     CDS             22407..23906
                     /gene="glpK"
                     /locus_tag="VGB88_14325"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX9648679.1"
                     /translation="MAEPDLVLALDQGTTSTRAIVFDRAGTARAEARRPLRQVYPRPG
                     WVEHDPEDIWQDAVAVMREALARARIEARALAGIGIANQRETTLMWQRDGGRPLHNAI
                     VWQDRRTAAACDRLHAAGAGERIAALTGLVIDPYFSATKAAWLLDTVAGARAAAERGA
                     LLFGTVDSFLLWRLTGGKAHATDATNAARTQLFDIHRRAWSDELLALFRVPGAALPEV
                     KDSAGMFGIAAADVLGAEVPVLGIAGDQQAAMVGQACLAPGMVKSTYGTGGFALLNTG
                     TWAVASRARLLTTIAYQIAGAATYALEGSIFVAGAALQWLCDGLGVLGDVADSEALAA
                     GADADGAVVMVPAFAGLGAPYWDAHARGALFGLTRDVGVADLARAALDSVAFQTRDLI
                     EAMAADGAGPPTALRVDGGMAANAWFLQRLADLIGVPVERPVETETTALGAARLAGLA
                     AGLIDGLDALAGEWRRECRFEPALDADHRDRLYRRWQDAVARVRESAGG"
     gene            23982..24323
                     /locus_tag="VGB88_14330"
     CDS             23982..24323
                     /locus_tag="VGB88_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9648680.1"
                     /translation="MSALAALAQDTRLTVFRLLARQAPAGLPAGAIARRIGAVPSTLS
                     FHLSQLEAAGLVRSRRRQRQVVYAVDAAGIRRLLAFLTEDCCGGQPELCGGVPAATSR
                     EEYRPAKVEET"
     gene            24323..24859
                     /locus_tag="VGB88_14335"
     CDS             24323..24859
                     /locus_tag="VGB88_14335"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9648681.1"
                     /translation="MADKVINVLFLCTGNSARSIIAECVINRLGRPRFRGYSAGSHPS
                     GAIHPFALDLLRRRSFPTSALRSKSWDEFARAGAPELDFVFTVCDRAAAETCPVWPGQ
                     PMSAHWGLPDPVAVVGSEAERRFAFADSLRMLIQRISIFIKLPFEALDRLSLKQRLDE
                     IGAAGRTAAQVEPGPGHD"
     gene            24852..25565
                     /locus_tag="VGB88_14340"
     CDS             24852..25565
                     /locus_tag="VGB88_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX9648682.1"
                     /translation="MTDFGLGRRLCAELVGTAILLATVIGSGIMAERLAGGNAALALL
                     GNTIATGAILTVLILVLGPISGAHFNPAVTLSFVVQRRMTWTDGAAFALAQVAGGLAG
                     MMLAHAMFEMPVVMIGANARTGAGQWLGEAVATFGLVATILGCLRVRPEAVAYAVGLF
                     ISAGYWFTSSTSFANPAVTIARSFTDSFTGIRPADAPAFIAFELIGALAATGLVVWLH
                     APRRDAARDEPAAVAPPGD"
     gene            25897..>31004
                     /locus_tag="VGB88_14345"
     CDS             25897..>31004
                     /locus_tag="VGB88_14345"
                     /inference="COORDINATES: protein motif:HMM:NF017889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HEX9648683.1"
                     /translation="MDVDAATAAAVPDIVAGATRILEIGPDNVVRLADGRIITDAVYL
                     RAGDDLVLRVPDGATYVIKGYFALTDRPDLVSDDGGRMTPGMVDAFTLNHQPEQVAQA
                     SEALAAGAVATVAVLEGSATVTRADGSVVPLSEGAPIYQFDIIESGADGAVRLLFIDN
                     TVFAIGPGARLTIDDFVFDTAGDGSVSAFTMLKGMFAFVSGQIADNDPSDFVLTTPVA
                     VVGIRGSAGLGDVNEPGTPSRFTVDEPTLVVTNNGGSVILDQRFATTNVTDINTPPSA
                     PEILDEDEVKTIYGPMGGVGPSFLSDESNGQDEQSAPQGPGDQGAAPGGVPDVVTAAG
                     PASGGQSGPPPSDGGTSQFTEMDSDQVLIHDLGRAGTTPTATGGTAGSSGGIGGGAEP
                     SPGPTSPSNQPATGGGSQPPPDQGGSPPSEPPGETPLFWVGGPGDDFQVGTSLADSLD
                     GAGGNDTLLGLGGNDTLIGGTGNDSLVGDDGDDSVTGGDGDDTIVAGSGQGNDTYLGG
                     GGDDTLDYSSAVTPLSIDMGEGTTVGGPEIGNDVFDGIEHVIAGAGDDTIAGDSNANM
                     IDGGGGADRLDGGGSSDTLLGGDGDDVLTYDPADGQVDGGGGFDTLRPPEGSVIDLTA
                     EVRPDLSGIEAIDLTDLDADTLIIDGASLLDLVGPGGALRVLGTAQDSVIAGGGWTEN
                     GVQAIDGETYRTFTQNGATLLVDTDIDRADIEPFASPPTLSVQPAQANENQPVPLVID
                     AALVDTDGSETLAIQIENVPAGAALSAGADQGDGIWLLAPGDLAGLTLDLPPDFDAAF
                     DLQVTAIATETEDGSFAETSANLTVTPTPDLSIADAIVVEPAFGAVVQVQGIGANGGT
                     LVDGLGGPAGFGENTFPANDDDSVFVDVTAVFGAGGMNFFGTNFTGFWLNNNGNITFN
                     EPQAEFTPIAITGDTGNPIIAPFFADIDTLGSHENAPTPGGNSTGSNLVYWDIDAANG
                     AITITWDDVGYYAGHTDKLNAFQLVIYKVNDAGDFAFEFRYETIDWTTGDASGGEGGL
                     GGVVARAGWSAGDGVNFLELPQSGNEAAVLDLENTSNDGTAPDGNWVFLAEGGDVGNL
                     PSGVALVDVVLDAPSNDVVTVDYQTVDGTATSTGTLGDGDFDYQPVAGSLVFQPGETV
                     QTIAITIYGDNAVEPNEVFTVELSNATNAGIIDAQATVTMTDSTAPPAPVDDARSAFD
                     ADLVGSDGAVQYAGNLLDNDELGNALLAAQPVVDIAVLTAYPGGVSVDDSGPYVASSN
                     DGAWMLTVDPETGDYTFTLNQALDHQAFGDGDVIDLAFSYRIETDTGFQSDPATLTVS
                     VFDDAPVASQDADLAVLTTDGEIAGVNLLANDEVGGDGGEITAITYDDDGPVTVPLVE
                     GSVTVLTALGGTLTVNADGSWSYQPARLIGAPQATDSFTYTLTDNDDDGAQAIQNVTV
                     TEVPLVAPVVDLDGPGGGTGFDAGTFVEGGAPRAVVDADATISDVDSANLLSLTATLT
                     NPLDGPDEALAADISGTAIVANYVSETGVLTLSGLDSVANYQAVLRTLTYQNTSESPD
                     PTQRVIEVQAEDEDEQLSNVAAATIALLAVNDNPVAVTDAYAADEDSILTVNAAGGVI
                     DNPVTFDSDPDGDALSVVAFDAVSTLGATVVMQADGSFTYDPTGVAALQQLGAGEDTV
                     DSFTYTIGDGNGGQDTASVEVTVSGADEVATEGN"
BASE COUNT         4618 a        11081 c        10795 g         4510 t
ORIGIN      
        1 gacctgacgg cgctcgcggc cgcgaagttg actcctccgc gccgtgccta gaccgtcgcc
       61 gctcaagctg cgagaggact ccccgggtgc tggattaaat ttgaagatct cgttcccggt
      121 ccaatcgagt gtctttcaac gttaccgatt tccattttct tacatccaag catctgcccg
      181 ggctaagcaa cgtggctttc tcactcacca aaaccacgtc caatccattg atgcgcctca
      241 caaaaatggc agagcccccg attgtgagta cctcttgaaa ccaatgtgac aatcttgtca
      301 gaatgggccg cgagaagaag aggcgctccc cttgggcgtg cggcgcatgc tctggggcga
      361 cgcccggggg aggggtgtga tgactctcga ttcgggacaa gccctgcgtc gcgagctcct
      421 gctcaagctg ctcgaggtat gcgagcaacc ggaggttgcg ctcgctgcgg cacggcgcat
      481 ggaagccttc atacttggcc ggccatcggc aggacctgat gctcaggaag ccgcgtccgc
      541 agaggaaggc gcaggcgtcg agggcgatgc ctatcttgcg cgggtgctgg gcccggtcac
      601 tgagaaaacc gatcagccgt cgctgagagc gccatcgacg ggttggcccg gccacaccgc
      661 cgctccatcc acggcctcac cgatcagtcg ccggcgctgg atcgatcgat ttgatcgact
      721 gctgagggaa caatgggatc ggggtgtacc ggttgcggaa atcgccgccc ggctcgatcg
      781 aacaacggca agtatcaacg gccgggtacg ggtcctgggg ctggccaagc ggcgcgggaa
      841 cacggcgcag ggcaagggca cgcggcccgg caagaacgac gcccgcaaca gcggcggcgg
      901 tctctccggc ggcggtcgaa aagcggccgg cgcgaaggcg ccccggccga ggtcgtgcgc
      961 gcccgacacc cctgaccgcc cggcgaaggc ggccgagggg aagctcattg ccgagtttat
     1021 cgccatgcac ggcgtcaggc gcgagccggt cacgatcgac tcggtggtga aatttttgca
     1081 gagccgcggc cactgcgtgg tgcgctcggg ccaaggtcgg ttcgagctcg acaaccgcga
     1141 gcacctcagc gcccgcgagc tgcttgtgcg tgccaacatt gcaaagcagc gcatgaggca
     1201 gccgaagttc cccgacagcg tcctccggag cgccggcagc gccgaggaag tcgcctgagg
     1261 gggaagcgac gtcgatcgcc cggccgggaa ccggaaagcc aggatcgccc gggccgcgac
     1321 ctagctgtaa atcaggctgg gcagccaggt ggccatggcc ggaaacgcgg ccaggatcac
     1381 cagcgtcaat agctgcatgg ccacgaacgg cactacgccg cgatagatgt cggtggtctt
     1441 gacctccggc ggcgcgacgc cgcgcaggta gaacagggcg aagccgaacg gcggcgtcag
     1501 gaacgaggtc tggatgttga ccgcgatcat gatcgcgaac cacaccgggc tgatgccctc
     1561 gttcatcagg atcgccgggc cgatgatcgg caccaccacg aacacgatct cgatgaagtc
     1621 gaggaagaag ccgagcacga acatcagcgc catggtcaag agcatggcgg tgaacacgcc
     1681 gccgggcagg ccgcgcagga agtcgccgac gatctcgtcg ccgtgaaagc cgcggaacac
     1741 caggctgaac agctgggcgc cgatcaggat gacgaacacc atcgacgaca ccttggcggt
     1801 cgagcgcatg acgtcgcgca ggttgttcct ggtcagctgg cggcgcgccg cggccagccc
     1861 cagcgagccc atggcgccga caccggcggc ctcggtgggc gtcgccaccc cgaccaggat
     1921 cgagcccagc accagcacga tcagcagcag cggcggcagc agcaccgtga cgacgcgccc
     1981 ggccatctcg ccgcgcgaga tcgccgcccg ctccgccgcc ggcaccgacg gcatgcgatg
     2041 gggatagatg ttggcgtaga tgaactgcca gacgatgtac agcgcgacca gggtgaagcc
     2101 cgggatcagc gcgccgacga acaggtcggc gaccgagacc ggctcgggcg cgaagttgcc
     2161 ggccgaaagc tgcgcctgct gataggcggc ggagatctgg tcgccgagca ggatcagcac
     2221 gatcgaaggc gggatgattt ggccgagggt gccggcggcg cagatcgagc cgcaggcgat
     2281 cggcgcgcca tagccgcgcc tgagcatggt cggcagcgac aacagcccca tcgtcaccac
     2341 cgttgcgccg acgatgccgg tggacgccgc cagcagcgcg ccgaccgcgc tgaccgcgat
     2401 gccgagcccg ccgcgcacgc caatgagcag cagcgccatg gtatcgagca gcgcctcggc
     2461 gagcttcgag cgctccagca tcacgcccat gaacacgaac agcggcaccg cgatcagcag
     2521 gtcgttggtc atgatgccgt agatgcggtt gggcagcgcg gcaacgaacg ccagctcgaa
     2581 gatgtcgaac gcggccccga ccagggcgaa caccagcgcc acgcccgaca gcgtgaacgc
     2641 aacctggaag ccgagcatca gcgccgcgca tagcgccagg accatggcca ggatgcagat
     2701 ctcgtagatc agcacgtcgg ccccctacgc ggcgtgcgcc cgatcggtac cggtatgccc
     2761 gcgcgggatg cgcaacgccg ggaccgtggt cacttggccg cctatcgatc tgatccgaac
     2821 tagagggcgt gctcgtgcgg ctcggcaacc tcgtgctcga cgccgcgcag catggcgagc
     2881 gcgtgaatga tgcgcgagac ggcctgcaag atcatggtgc ccgcgaacac ccagatggtc
     2941 gtcttgagca ggtagaccgc gtgcaggccg cgttcctcga gcgatccttc gttctcggcc
     3001 cagctgttga gcacgtaatt ccacgagaag tagacgatca ccgcgcacac cgggatcagg
     3061 aacacgatgt tgccgaagat gtcgaccttg gctttgctgc gctcggacat cttgacgtag
     3121 agcacgtcga cccggacgtg ctcgtcgtgc aacagcgtgt agcccgcgca cagcaggaac
     3181 acgaacgcgt gcatgaaccg gaccgattcc tgcatccaga tgaaacccag attgaagccg
     3241 tagcggagca ccacgatcat caccgtgacc agcaccatga ccacggtcag ccaggcgaac
     3301 aggcgaccca tgtgctcgtt cagcgcatcg atggcgcggg cgacgccgag cagggctctc
     3361 acggccggct tcgctccccg cagccggaat cgtcaagcgt ttcggtcatg gccgtcatcg
     3421 tccccgcctg tccccatcag ccccgccggt cggccggtcc cggcccgatc cgcgggcggc
     3481 cgggccacgg tagagatttc cgccgccaac tcaagggcct tggaccggcc tgcggccgcg
     3541 ttgcggcggt gtgaggcgct tcacagatgg cgtgtgatcg ggtcaacagc ggcgccgcgc
     3601 gcgttccggg ataacacgcc gtccccctcc ccgtcgtcga tggcgttgct ggacgaccgg
     3661 aaatcgagtc gaacatcgga gcaaaaaatg aagacgtcgt ccccgacctt cctgcgctcg
     3721 accatccctg cccttgccct cgccctcgcg atcggcagca ccgccgcgac cggcgcgcat
     3781 gcggacggtg tccgcaccaa ccacgcgccc caggtcgccc gcggctacgt ccgccatgcc
     3841 gacgaggtca ccgcgttcgg gctgtccgac cacgcggcga tcgcggtcgc cgcgcccagg
     3901 tccggtcgcg gttacgtccg ccgagcggac gaagtggccg cgttcgggct gtccgaccgg
     3961 aagccgatcg tggtcgccgc gccgagggtc ggtcgcggct acctgccgcc gttcgagacc
     4021 ggcctcgccg ccggccgttg acctgacgca atccggccgg cccgggagcg cgccctcgcc
     4081 gcccccggtc cggccgggtc cgcgcgctgc cggcgcggcc cgagggcgcc ggttcccagc
     4141 cgctcgcaac tcggctatag tcggcgcgga ttcgccccag ccgaaggccg atgtccgcga
     4201 cgatggatgc tgtcctggac tccctttgcg acgatccacg ctgctgccgg gagcgtttgc
     4261 tgcacgccgc cggcattttc tacgccgagc gcggcttcga tcgggtctcg actcgcgaag
     4321 tgacccgcgc cgccggcgcc aacctggccg cgatcgctta ttacttcggc ggcaagagcg
     4381 gcctacgcga ggccgtgctc gatcatgtcg tcatggccag ccgcgagcgc atcgccggca
     4441 tcttcgagcg gttgtcgaag gcggtcgaag agtcgggcgg cgcgcccgcg cgcctggccg
     4501 aggcgaccta caggttcgcc gacgagttcc tccacgccac gctgccgctc agccgggaga
     4561 cctggtgggt gaccgtcatc acccgggcga tgggcaacct gagcgcgggc gaggagcgca
     4621 tctacgaggc ggtgttcagg ccggtcaacc ggaccgtgca gaagctggtg gccgccgcca
     4681 ccggcgaatc ggaccacgat cggctcggcg tgctgaccga ggcggtggtc ggcgactttc
     4741 tcaccttctg caagaactcc tcgctggtgc tgcgcagcct gggatgggac ggctacagcc
     4801 cggagcgcat cgaccggatc gtgccggtgg tcgcgcggcg catgctgggc cggctgggcc
     4861 tgccgctgcc ggcacagctc gcgccgttgc gtgccggcgc gccggcggcg ggcgaattcc
     4921 gcgcgcgggc ggtcggctcg gcctgatccc gggcgacttt cagtcgaact tgttggtcga
     4981 cgggaatccc ttcggtgcca ggcgcccggc attgccgcgc ttaccctgcc agtcggcgac
     5041 gtcctcaagc gtgcgcgtgc gcgatccctg ccgccagccg aggccgtccg cggcgcggaa
     5101 gacgcgcgca tccgacagcc cgccgtcctt gtacttctgc aggatcacgc cgcgcccgcg
     5161 cgcgagcacg ggcacctcgt cgaggccgaa caccaccagc ttgtggttgt cgccgaccac
     5221 cgccacccgg tcgccctcgg cggcgacgca caccgccgcc tcgaccgcgc cgcgcacgtt
     5281 gagcacctgc cggccgccgc gcgtctgggc gacgacgtcg gtctcctcga ccacgaagcc
     5341 gcggccgtcg ctggccgcga ccagcagccg ccgctgcggg cggtggacca gcagggcgac
     5401 cacgtcgtgc tcattggcga ggtcgatcat caggcgcagc ggctcgccga agccgcgccc
     5461 gccgggcagg cggtcgcaag ccagagtgta gaagcggccg ttggtcgcga acgccagcac
     5521 tttgtcggtc gattgggcgt gcaggcgccg ccacggccgg tcgccttcct tgaacttgat
     5581 ctcggcatcc tcggccagat ggccgcggac ggcgcggatc catcccttct cggaacagat
     5641 gacagtgacc ggctcgcgct cgaccatgcc ctcgagcgag accacctcgg cggccggcgc
     5701 gtcgccgatc tcggtgcggc gcgcgccgag cgcggtgtcc ttgccgaacc gccggcggat
     5761 gtctcggacc tcgtcggcga ccatgcgcca ctgggcgcgc tcatcgcgga gcagcgcctt
     5821 gacctgcttg cgctcgccgg tcagcgcctc gcgctcgctg cggatctcga tctcctcgag
     5881 ccggcgcagg ttgcgcaggc gcatgttgag gatcgcctcg gcctggacgt cgctcagcgt
     5941 ccaacgcgcc atcattgtcg ccttcggctc gtcgtcctcg cggatgatgc ggatcacctc
     6001 gtcgaggttg aggtaggcga ccagaaggcc gtcgagcacc tcgagccggc gctcgatccg
     6061 ctccaggcgg tggcgggagc ggcgcagcag cacctcgcgc cgatggtcga ggaacgcctg
     6121 cagcgcctcg ctgagcgaca tcacgcgcgg cgtgttggtg gcgtcgagca cgttcaggtt
     6181 gagcggcacc cgggtctcga gctcggtcag cctgaacagg gtctccatca gggccgcggg
     6241 ctcgaccgtg cgtgcgcgcg gctcgagcac caggcgcacg tcctcggccg actcgtcgat
     6301 cacgtcggcg agccacggca ggcgcttggc cgccatcagc tcggcgatct tctcgaccag
     6361 ccgcgccttc tggacctgat agggcagctc ggtcaccacc acctggtact ggccgtggcc
     6421 gagcttctcg atcccccagc gcgcgcgcac gcggaacgcg ccgcgccccg tccgataggc
     6481 ctcgatcacc gccgcgcgcg gctcggcgag cacgccgccg gtggggaagt caggccccgg
     6541 caccatctcg accagggtct cgatgcacgc gtttggggtc ttgatcaggt gcagcagcgc
     6601 ctggcacagc tcgtcggcgt tgtgcggcgg gatgctggtc gccatgccga ccgcgatgcc
     6661 gctggcgccg ttggcgagca ggttggggaa cgccgccggc agcaccaccg gctcgctgtc
     6721 ctcgccgtca taggtggcgc ggaagtcgac cgtgtcctgg tcgatgccgg cgagcagcgc
     6781 ctcggcgacc tcggtcaggc gcgcctcggt gtagcgcatc gccgcggcgc cgtcgccgtc
     6841 gacattgccg aaattgccct ggccgtcgac cagcgggtag cggacggcga agtcctgggc
     6901 gagccgcacc atggcgtcgt agaccgcgac ctcgccatgc gggtggtact tgccgatcac
     6961 gtcgccgacc acgcgcgcgc atttcttgta gcccgaggcg gggtcgagcc tgagctggcg
     7021 catggcgtag agcaggcgcc ggtgcaccgg cttgaggccg tcgcgcacgt cgggcaacga
     7081 gcgcgacatg atcgtcgaca gggcatagga caagtagcgc tcgctgaggg cgtcggcgaa
     7141 cgcgaccgga atcacttgcg ccgcttggga ttcacgtgtc atggctgata catagcatag
     7201 cgttcgatgg tcaaccacca catgtggtgg acaagcgttg gaaacccgtg accagccggt
     7261 cgcgcgcgac gggcaggccg gagcccgcgc gcgcgatgcc gtggcggctg aggaaatggc
     7321 cggtgagcgc gagccccgca aggatatccg cgatctcgcc ggtgccgcgg cccgcggtca
     7381 ggaacgcggg caggcgcagc aaccggtcgc cgtacggcgt gccggcctcg gcgctgaccg
     7441 cgcgcccggt acgcggcgag acccaggcga gatcgtcggg acggccggtg accgcgcacg
     7501 ccgagaggtc caggccgaag ccgagctcgc gcagcagccc gacctcccag ccgacgtaag
     7561 ccgcccccca gtcgccgggc tcggcgtcgg aggcgagcgc ggcgagcaac gcgcacaggg
     7621 cgtggaacag cgccgggtgc ggctcgcgct cggccagcgc cgcatcgacc agcgccgccg
     7681 ccgatgccgc ggcggcgagg cgcagcgggt cgtcgagcag cagcgccgca taggcccgca
     7741 gcggctcgcc cgacaccgtc cccaggtgct cggcgaggcg cgcgcgccag cggcagctga
     7801 cgtggttgcc cggctgccac aggctgccgc cgcgccggcc ggcgccgccc gggaccaggc
     7861 cggcgtggcg gccgtggtcg cgggtcagca ggtggacgat cagcgcggtc tcgccgtggc
     7921 gtcgccgcga cagcaccacc gcctcgtcga tccattccat cgccggcagt caccgatcag
     7981 acgtcgtagt cgaggccgag gtcacggtag cgctcgggat cctcgggcca atcctcgcgc
     8041 accttgacgt gaaggaacag gtggacgcgc tggccgagca tggcctcgag cccggcccgc
     8101 gcggcgctgc cgaccgcctt gatctggcgg ccgcccttgc cgagcacgat catgcgctgg
     8161 ctgtcgcggc ggacatagat cacctggtcg atgcggacgc tgccgtcggc gcgctcgctc
     8221 caggcctcgg tctcgaccgc aatctcatag ggcagctcgt ggcggagctg caggaacagc
     8281 tgctcgcggg tgatctcggc ggcgagcagg cgcagcggca tgtccgccgc ctggtcctcg
     8341 gggtaaagcc acggcccggc cggcatgcgc cgggcgagca catgcagcag gtcggcgacg
     8401 ccgtcgccgg tcagcgccga gatcatgaag acgtcggtga acagcttgcg cccgtcggcg
     8461 agagtctcgc ggtcgagcgc ggcggcgagc gccagcaggc ccggccgctt gaccgcatcg
     8521 accttgttga tcgccgcgac cgcctggcgg ccctcggccg cgagccggtc gagcagccgg
     8581 cggctgtcgg cgccgatgcc gcctctggcg tcgaccagca ccaccaccac gtcggcgtcg
     8641 cgggccccgg cccaggcggc ctgcaccata gcccgctcaa ggcgcttcct gggggcgatg
     8701 atgccgggcg tgtcgatgaa cacgatctgg gtgtcgccgg cgctggcgat gccgagcaca
     8761 cgcgcgcgcg tggtctgcac cttgggcgtg acgatcgaga ccttggcgcc gaccaggcgg
     8821 ttgagcaagg tcgacttgcc ggcgttgggc gcgccgagca gcgcgacata gccgcagcgc
     8881 gtgccgacct cgcgcgccag ctcagccatc tgcgccggcg gcgaggcgtc gcagcatgct
     8941 cgcggccgcg gcgcgctcgg cctcgcgctt gccggcgccc tcgccctcgg ccgcgccgtg
     9001 gccggcgacc gtgaccgcga cccggaacag cggccggtgg tccggcccct cgctggccac
     9061 cagccggtag ctcggcaagc ccaggccgcg cgcctgcgac cattcctgaa gcgcggtctt
     9121 ggcatccttc ggcggcgtca cctggcgcac gatcagctcg cgccaatggc gctcgacgaa
     9181 ggcgcgggcg gcatcgaggc cgccgtcgac atagagcgcg ccgatcaccg cctccagggc
     9241 atcggccagg ttggccgggt tggtgcgccc gccgctcgcc tcctcgcccg ggcccaacac
     9301 caggaagcgg ccgaggtcga gccgtgccgc gactgcggcc aggccggcgg cgctgaccag
     9361 gggcgcgaag cgacgcgcga gcgcgccttc gtcctcgtcc gcgtagcccc gatagagccg
     9421 ggcggcgatc accagcccga gcacccggtc gccgaggaac tcgaggcgct ggttgttgac
     9481 ccggccgggg ccgacgctgg ggtgcgtcag cgcctgctca agcaggccgg ggtcgcggaa
     9541 ctcgacgccg agcgcggcgg ctgcgccggc cagggccgag cccggcacgc gcccgctcac
     9601 aacaggatgc cgaagcgcca gaacctggtc gcgaacggcc acttccagac ctcccagaaa
     9661 tgggccgttc cgtcggtcga gaagaacagg atctcggcgc gcccgaccag gttctcggcc
     9721 ggcacgtagc cgacattgga gaggatgcgg ctgtccgagg atccgtcgcg gttgtcaccc
     9781 atcgcgaagt aatggtcggc gggcaccacg tagacgtcgg tgttgtcgag cgggccgtac
     9841 ggattggagt cgatgatccg gtagccgatc ccgttgggca gggtctcgac gtactggtcc
     9901 accgcggtga cgttgccgat gcggtcgacc agctcgtagt cctcgatctg gcgccgctga
     9961 accggctgat cgttgatgta cagcagcccg tcgcgcacct ggatgtggtc gcccggcagc
    10021 ccgatcaggc gcttgatgta gtcggtcttg ttgtcgcgcg gcagcttgaa cacggcgatg
    10081 tcgccgcgct cgggctggtg cgccatgatc cgtccctcga tcacgccgat gtcgaacggg
    10141 aacgagtagc ggctgtagcc gtacgagaac ttggacacga acaggtagtc gccgatcagc
    10201 aggctcggga tcatcgagcc ggacgggatg tggaacggct cataggcgaa ggtgcgcacc
    10261 acggtcgcga tcagcaccgc gtagacgacg gtcttgaacg tctcccagaa cccgtcggcg
    10321 cgggatttct tggcgatgcg gccggaacgc tgggtcatgt gcggtcgcca attcggcttg
    10381 tcctgcgctc gcccgcggca gcccggggcg cggcgcgccg gcactttacg gcccaagcgc
    10441 ccggatgcaa acgcaaactc cgggtcggcc tcacgcgccg gacggcgccg ccggcaccac
    10501 cgagatgatc acgatggcgt gggcgagctc gcggtcgtcg gtcagcgaca cgtcgagcct
    10561 cgcctccatg cccgccggcg tgatctcgcg caggcgggcg agcgcgccgc cggtgagctc
    10621 gatcgtcggc ttgccgccgg gctggttgac cacgccgatg tcggcccagt ggacgccgtt
    10681 gcggaagccg gtgccgagcg ccttcgagca cgcctccttg gcggcgaagc ggcgggcata
    10741 ggagtcggcc cggcgcaggc gtcgctcgca ccgctcgcgc tcgacgggcg tgaacacgcg
    10801 atgggtgaat cggtcgccga accgctcgag cgcgctggcg atgcggctcg cgtcgcagac
    10861 gtcgtgtccg atgccgagga tcacccctgc cccctacggt cgcccggccg gcctcgcccg
    10921 atcacgcgct gcgcttgccg ctcgcctcgg cccgcgcctt gtccatgatc gcgcgcatcc
    10981 gccggatgct gctttccagg ccgccgaaga tcgcctcgcc gaccaggaaa tggccgatgt
    11041 tgagctcgct gacctcgacc atcgccgcga tcgggccgac cgtgtcgaag ctgagcccgt
    11101 ggccggcatg gcattcgagg ccgcgcgcca gcacctcgcg cgccgccgcc ttaagccgat
    11161 cgagctcggc ctcgcgccgt ccgccctcgg cgtcgcagta cgggccggtg tgcagctcga
    11221 cgacatcggc gccgagcgcc tccgcgaccg cgatctggac ccggtcgggg tcgacgaaca
    11281 gcgatacccg gatgccggcg ccgtgcagcc ggccgatgta gggctcgagc gcggcgtgca
    11341 gggcggcggc atccaggccg ccctcggtgg tcagctcctg ccgcttctcg ggcaccaggc
    11401 acaccttggg cgggcgggtg gcgagcgcga tgtcgagcat ctcagcggtc gccgccatct
    11461 ccaggttgag cggcaggtcg atctcgtcct tgagccggtg aatgtcggtg tccgagatat
    11521 ggcgccggtc ctcgcgcagg tgcgcggtga tgccgtcggc gcccgcctcg gcggcaagcc
    11581 gcgccgcgcg caccgggtcc ggatgccggc cgccgcgcgc attgcggatg gtcgccacgt
    11641 ggtcgatgtt gacgccgagc ctgaggtcgt gggtcatcgc tctcttatct cgccgccggc
    11701 cagctcgcgc gcctcgggcg cggccgcgtg gccggccagg gccgccttgg cctcgcgtgc
    11761 cgcccgccgc gccgcgcgcc gttccgcctt gcgcgcgatc cgggccgcgc gcatgtgctg
    11821 atagcgcgcg accgcgcgcc ggacaagcca gaaggatatc gcccaggccg ccagcgcaag
    11881 cagcgtgccg ccggtcatca tcggcaggaa cacggcctcg gggttgtcga agatgtagct
    11941 gatcgacatg ccctccggcg ccacctcggg caccaagtgc tcaaacccga gcagccacgc
    12001 gcccagccgg tagctggcca gccagatgac cgggaacgtc cacggattgc cgatcgccgt
    12061 gccgatcgcc gaggcgatga cgttgccgcc gatcagccag gcgatcagcg cggcgagcgc
    12121 gaagtggaag ccgatcaggg gcgtgaacga gaccgcggca ccgcaggcga agccggcggc
    12181 gaccttgtag ggcgagccgc gcaggcggcg cacgcggtgg ccgtaatagg tgctggcgcg
    12241 ccgccagccc agacgcggcc agatcagctg ccacagccgg tcgacccaat gcgggcgaat
    12301 ccgccggcct agcatgcgcg cgtccggatt tcagttgccc cgcgctcggg tatgctcagg
    12361 acgatcacgg acctcagccc tgcgcgcgct cggcacgata gatcgaggga tgcgacctga
    12421 gcgtggtgat gatgtcggtc aggtggcgca ggttctcgac ctcgatatcg accacgaact
    12481 cgaagaagtc gggcgagcgg ttgacgatct tgaggttgct gatgttgccg tgccggcgcg
    12541 cgatcgcctc ggtgagtgca ttgaggctgc cgggcacgtt gttgatcacg gtcacgatgc
    12601 gcccggtgtg taccgagggc ttgacgtcgg aggtgtccca cgacacgtcg agccagcgct
    12661 cgggcgagtc gatgaagctt tccagcacct cgcagtcgat ggtgtggatg gtcacgccct
    12721 tgccctcggt caccagcccg acgatgcggt cgccgggcag cggatggcag cagtccgaca
    12781 tgtgcaccgc catgcccggg gtcaggccct tgatcggcac cgcgccgcgg cggcggtcgt
    12841 cgagcctgcc gcggtcggcc acgaacggca ccaccttgcc cggcttcgcg gcctggtccg
    12901 gatggaccgc agccagcacg tggcgggcgt tgatgtcgcc gcggccgacc gcggcgaaca
    12961 ggtcgcccgg attgcgcttg gcgagcttgg ccagcgccgg cgccagcacc tggtcgccga
    13021 acagcttgtc ggcattaagg aaggtcttct cgaggatcga gcggccgagg ctggcatact
    13081 cgtcgttgcg ccgctggcgc acgaagcggc ggatgcgcgc gcgcgccttg ccggtggcca
    13141 cgaagttctc ccagttgggg tccggcgccg gcacgctcga gcgcaggatg tcgacctggt
    13201 cgccgttctg cagccgggtg cgcagcggcg ccagccggcc gttgatcttg gcgccgacgc
    13261 aggtgtcgcc cacgtccgag tgcacggcgt aggcgaaatc gaccggcgtc gatcccgccg
    13321 gcaaggtgat caggtcgccc ttgggcgaga agcagaacac ctggtcggcg aacatctcga
    13381 gcttggtgtg ctcgaggaac tcctccgggt tggaggcgtg ctcgagaatg tcgagcagat
    13441 ggcggagcca ccggtactgg cggccgtcgc gcatcaggcg gccgtcggcg tcctgcttgt
    13501 acagccaatg ggtggcgacg ccgtactcgg cgacctcgtt catctcgcgg ctgcggatct
    13561 ggatctcgac ccggcgccgc tgcgggccga tgaccgaggt gtggatcgac cggtagccgt
    13621 tgggcttcgg ggtcgagacg taatccttga accggccggg aatggtgggg taggcctgat
    13681 ggacggcgcc gagcgcgcgg tagcacgcgg cggcgtcggc caccaccacc cggaacgcga
    13741 tcacgtcggc gatctgctcg aacgggacgt gcttgcgctc catcttgcgc cagatcgagt
    13801 agggccgctt ctcccgcccc atcacctcgg cctcgatgcc ggcggcggcg agcgtctgct
    13861 tgagctcgtc ggcgatgacc gggatcagct ggccctcggt ctcgcgcagg aagtcgagcc
    13921 gcttgaccag cgactctcgg gcctcggggt agagctcggc gaacgacagg tcctcgagct
    13981 cgtccttcag ccgctgcatg ccgatgcgct cggccagcgg ggcgtagatc tccatggtct
    14041 cgagcgcgat ccggcggcgc ttgtcggcat cctcgatgtg cgacagcgtc ctcatgttgt
    14101 gcagccggtc ggcgagcttg accaggagca cgcgcaggtc ctcggacatc gccagtagaa
    14161 acttgcggaa attctcggcc tgccgctcgg agcgcgactg gagctcgatc ttgctcagct
    14221 tggtgacgcc gtcgaccagg cgggcgatct cgggcccgaa ctgggccgcg atctcagcct
    14281 cggtcgcgac cccgtcctcg acggtgtcgt gaagcagcgc ggtgatgatc gaggcggcat
    14341 cgagcttgag gtcgaccagg atgccggcga cctcgagcgg gtgggagaaa taggggtcgc
    14401 cggacgcccg cctctgggcg ccgtggcgcg acatcgcgaa cacgtaggcg cggttgagcg
    14461 cctgctcgtc ggcattggga tcgtaggcgg cgacgcgctc gaccagctcg tactggcggg
    14521 tcgtggggcg gcccggcgag gcgcgcgccg gcgccctcgc cggctcggtc gttcccggtc
    14581 gcttgcccct ggtcccggcg gagcgggttc ccttagcttg gcgccgcggc gcgctcaatc
    14641 gtcgtcgtcg gacgactcga cggcagcctt gtcctgcccg cctccggtct cgacgatctg
    14701 catgccgggc agctcgccgc ccatcgcttc gagctcgctg agcaggtcac cctcgtccat
    14761 cgccgcggcc tcttcctcgg gctcgtcgat ctcggcgtgg cgctgcaggc cgcgcaccag
    14821 cgcctcctgc aacgcctcgg tgcggacggt ctcctcggcg atctcgcgca gcgcgaccac
    14881 cgggttcttg tcgttgtcgc gctcaaccgt caggctgccg ccggccgaga tgtcgcgggc
    14941 ccgctgggct gccagcatga ccagctcgaa gcggttggga atcctgagaa cgcagtcctc
    15001 gaccgtcacg cgcgccatat ttcgtcgctc tccaaaaaat cggatagggg gctgaaccga
    15061 ccaaatatat ggagccgcac cgcacaaagc aaggtgcatc aatgccttcc ggcaggtttc
    15121 tgggccgacg gcctgccctt tcggggctcc accggagccg tccgatcccc agcttccggc
    15181 tcccccacac ccagaggccg aatttcctgg tccggcggca gccttgcggc gagcgccgcg
    15241 accgtctcgc cgctccccgg caggcgccag ccgggcgcga tctcggccag gggcagcagc
    15301 acgaaggccc gctcggggac cctcggatgg ggcagcagcg gcgccgccgg ccccgcgcgg
    15361 accaggtcgc cataggccag cagatcgagg tccaccgccc gggcggcgtt gcgggccccg
    15421 cgcacgcggc cgaaatcgtg ctcgatggcg tgcagcacgg cgagcagcgc ttcggggccc
    15481 agcccggtct ccaccgccgc cacgccgttc acgtaccagg gctggtcgga gcgcggcacc
    15541 ggcgcgctct cgtaccagcg cgagcgccgc accacccgca cgccgcggcg ctccagttcg
    15601 gcgagcgccg cctccagcgt ctggcgcgga ccgccgaagc gctcgctcgg caggttggcg
    15661 cccaatccta tcaaaatggg tccatctccc cgccttaccc ggtgatcgct gggcgatttg
    15721 tcggtcatcc cttgcctgcg cgcttgtctg gcgacgaaag cgccctaatt atgaagctgt
    15781 taccggcgcg agtcgccggg gcatcctgcc tgcccacgct gcgaagactg gtccgcctcg
    15841 gcaaattcat tctcttcacg gcacattact taggacagat catgatactg aagttccacc
    15901 ctcaggagcg cgttgcgatc ttcatcgacg gcgccaatct ctacgccgcg gcccgcgccc
    15961 tcggcttcga catcgactac aagcgcctgc tcgacacctt caccggcgcc tgccacctga
    16021 ttcgcgcctt ctattacacc gcgctggtcg aggaccagga gtactcgccg atccggccgc
    16081 tggtcgactg gctggactac aacggctaca ccatggtgac caagccgacc aaggagttca
    16141 ccgactcctc cggccggcgc aagatcaagg gcaacatgga catcgagctg gccatcgacg
    16201 tgatggagat ggccgagcat gtcgaccata tcgtgctgtt ctccggcgac ggtgacttcc
    16261 gccgcctgat cgaggcggtc cagcgcaagg gcgtgcgggc atcggtcgtc tcgaccgtgc
    16321 gcacccagcc gccgatggtc gccgacgagc tcaggcgcca ggccgacaac ttcatcgagc
    16381 tcgaggacct gtccgagcac atcgcccgcg acccgatggc ccggatggcg cgccgcgccc
    16441 aggaggcggc ggacatcgag gcggacgagg acgtggagga ggagctcgac ctcgggcgcg
    16501 cgtgatccgg ggcgcccggt acggcccggg cgcgactgcc cgcgctgtcc gcgcctggtc
    16561 gcattccgcg ccgccaaccg cgcccggttc gccggctatc acaacgcccc ggtcgactcc
    16621 ttcgggccgg ccgcggcgcg gctgctggtg gtcgggctgg cgccgggcct gcgcggcgcc
    16681 aaccgcaccg gccggccgtt taccggtgac gccgccggcg agctgctgta tccgacgctg
    16741 atcgcccacg gcttcgcgcg cggcacctat cgggcccgcc ccgacgaccg cctcgagctg
    16801 gtctcatgca ggatcaccaa cgcggtgcgc tgcgtgccgc cgaacaacaa gccgaccggc
    16861 gccgagctgc gcgcgtgccg cggcttcctc gccgccgaga tcgcggcgat gccggagctg
    16921 agggcggtgc tggcgctcgg ccggatcgcg catgatgcgg tgctcgccgc gctcgggctc
    16981 ggccgcgcgt ccacgccctt cgcccacggc gcccgccacg cgctcgccag cgggcaggcg
    17041 ctgttcgaca gctaccactg ctcgcgcctc aacctcaaca ccggccgcct gacgccggcg
    17101 atgttcgacg ccgtcatcgc ccgcgtccgc gccgatttag ctcttctacg ccaaaaagtt
    17161 agcttataac aaaatcttaa gagctatttt tccagcgcgc tctcaatagc gtcggtgacc
    17221 gtgccggact cgggtttgac ctgcgacggc cacgcctcgg tcaccgcgcc gctcttatcg
    17281 atcaggtact tatggaagtt ccagcgcggc tcggcggcct cgccgagcga gtcggcgatc
    17341 cagcggtaga gcgggtgggc gtgcgcgccg accaccgtct gcttggcggt catcgggaag
    17401 tcgacccggt agttggcctc gcagaactcc ttgatctcgc cctcgtcctt cggctcctgg
    17461 ttgccgaagt cgttggacgg caccccgagc accaccaggc cgcggtcgcg atagcgccgc
    17521 cacagcgcct cgaggccctc atactggggc gtgaagccgc agaacgaggc ggtgttgacc
    17581 agcagcacga ccttgcccgc aaaccgggac atcggcagcc gcttgccctc gatcgaggtg
    17641 aagtcgaagt cgtgcgcggt catcgccagt cccggtgtcg ccgcggccaa tgcgacggcc
    17701 aacgccgcgc cgccggcgag cttgcctatc atgcccagca taaactgcct cctcgatgat
    17761 ttgccagccg gtcagctgcg cgcacgcatc aggcgcgcct tgtcgcgctg ccagccgagt
    17821 tccttctcgc gctcgcgctt gtcgtgcagc ttgcgcccgc gcgcgatgcc gagctccacc
    17881 ttggcacggc cgcgctcgtc gaaatagagc ttgagcggca ccagcgtgta gccgcggcgc
    17941 tcgaccagcc cgcgcaggcg ctcgatctcg cgccggtgga gcaaaagctt gcgcgggcgc
    18001 aacgggtcgt ggttgaggcg gttggcgggc ggatagtggg cgatgtgcga gttgaccagg
    18061 accatctcgc cgtcccggcg ctcggcgaac gcatcgccca ggctcgcccg gccctcgcgc
    18121 agcgacttga cctcggtgcc gaccaggacc aggccggcct ctaccgtgtc ctcgatgatg
    18181 tagttgtgcc gggccttgcg gttctgggcg acgatgcgct gcttcaggtc gccgcccgcc
    18241 attgccggac attctcgctg agcaggccgg cggccgcgag cgccgcccgc accttagcct
    18301 tggtcgcctc gccgacctcg accatcggca ggcgcagctc gcccgagcac agcccgagca
    18361 ggctcgcggc gtactttgcc ggcgccgggc tggtctcggc aaacaggtgc gtgtgcagcg
    18421 gcatcaagcg gtcgtgaagc gccagcgcct cggcggcgtc gcccttggcc caggtctcct
    18481 gaacctcgac gcacaacgcc ggcgcgacat tggcggtcac cgagatgcaa ccgttgccgc
    18541 cattggcgag ataggcgagc gcggtgccgt cctcgcccga caggaacgag aactcgccct
    18601 cgatcgcctg gcgctcgagc agcgggcggg tcaggtcgca ggtcgcgtcc ttgaccccga
    18661 ggatgttcgg gatcctggcc aggcgcgcga gcgtgtcgac gctcatgttg accaccgacc
    18721 ggccgggaat gttgtagacg atgatcggga tgtcggccgc cgccgccacc gcctcgaagt
    18781 gccggtacat gccctcctgg gtcggcttgt tgtagtaagg cgtgaccacc agcgcggcgt
    18841 cggcgccggc ctggtaggcg tgccgggtca ggctcaccgc ctcctcggtc gagttcgagc
    18901 cggtgccggc gatcaccggc acgcgcttgg ccacggtctc gatgcagacc tcggtgaccc
    18961 ggcggtgctc ggtgtggctg agggtcggcg actcgccggt ggtgccgcac ggcaccaccc
    19021 cgtgcgcccc ctgcgtgacc tgccagtcga cgaaggcctg gaaggtcttg tcggcgacct
    19081 tgccgtcctc gaacggggtc agcaacgcgg tgatcgatcc cttgaacatg gccaaaccct
    19141 cctcacccgg cgcgcgccgg cgggcgtgcg gcgataccca gcgcaaaata cccgcgccgc
    19201 ccccggccgg caagccggcc tcagcgactt cggtcggatt gagcgacggg cgagccagcg
    19261 tatattgacg ttcctgtcgt ttccttgctc atgcattctg tcattctgtg acgatgtcgt
    19321 ttcgattgcc gatcttggcc ctggcggcct tgctgatggc ggcgacggcg ccggatgcgg
    19381 cgcccgccgg caagaccccg cgcgtgctgt ccgatgccgg cctcgcgcac gcgcgctcgg
    19441 cgctcgagca cgtcgggcac aagcgctggc gcgaggcaca atacgatgcc ggcagggccg
    19501 gcgagccgct ggtcgccaag ctggtggagt ggcggcggct tgccgacgac ggctccggtg
    19561 cctcgttcac caccctcgcc gccttcatcg acgcgaaccc gcgctggccg ggtctggcga
    19621 cgctcaggcg caatgccgag aaggcgatgc ccgggacgat ggcggaccgg gatgtggtcg
    19681 cctggttcga gcgtcatccg cccgcaagcc ctaccggcaa gcggcgcttc gccgaggccc
    19741 tgctcgccgc cggcgatgcc gggcacgggc ttgccctgat ccgcgacgcc tgggtcaacg
    19801 gcgatttcga ctcgcgccag gagaaggcgt tcctcgccgg ccatgccaag cgtctgcggc
    19861 ccgaggacca cgtcgcacgc ctcgaccggt tgttgtggga cgcgcgcgtg cgcgaggccg
    19921 agcgccagat gccgcgtgtc gatgacgccc atcgccggct ggcgctggcg cgcatcaggc
    19981 tgatggccca ggccggcggc gtcgatcctg cggtggcgcg cgtgccgccg gcgctggcgc
    20041 aggacgcggg gctgatctac gagcggctgc gctggcgccg gcgcgccggc ttcgacgacg
    20101 gcgcgcgcga gctgctcgat gcccagccgg ctgacccggt gcgcgccggt cgctggtgga
    20161 ccgagcgcca catcctcgcc cgccgggcgc tcgataccgg cgcgatcacc gccgcctatc
    20221 gcgcggcggc gcatcacggc caggccgggc cggcggagat cgcggaagcc gagtggctgg
    20281 cgggatggat cgcgttgcgc ttcctcgacg agccgctgat cgcctatcag cacttcgtcg
    20341 acatgcacca ggtggtggcg atgccgatca gcatcgcgcg cgccgcctat tgggccgggc
    20401 gcgcggcgga cgccgccggg gtgcccggcg aagccgaacg ctggtatcgg cgcgcggcga
    20461 cccagccgac cgcgttctac ggccagctcg ccctgctcgc cctcggcgcc gacgagctca
    20521 ctctgccggc ggacccggtg ccggggccga acgtggtcgc cgcgttcgag cgcgacgagc
    20581 tggtccggct cgcgcgcatc ctcggcgagc tcggcgaggc cgacctgatg tcgcgcgtca
    20641 tcgcgcgcat catcgacgac gccgactcgc ccgcgcttgg cgatctcgcc gcgcgcctgg
    20701 ggctcgacta cggccttgag ccggtcgcca tccgggcgtc gaaatacgcc cagcgccagg
    20761 gcgtggcgac catcgccccg gcctatccgg tgccgcccgc ggtcgcatcc cacctccagt
    20821 ccgacagcgg ccggctggag ccgggcctga tgctcgccgt cgcccgccag gaaagcgaga
    20881 tgaacccgaa cgtggtcagc agcgccggcg cccgcggtct gatgcagctg atgccgccga
    20941 ccgccgcgat ggtcgcgaag cggctcgggc tgccctacga cgcggcgcgg ctgaacggcg
    21001 atccgcgcta cagcctgcgg ctcggcggcg actacttggc cggcctgatc gacgacttct
    21061 ccggcgccac cgtgctggcg gtcaccggtt acaacgccgg gccgtcgcgg gttcgccagt
    21121 ggatgcgcca gaacggcgac ccgcgcgatg cggatgtcga cgtgatcgac tggatcgagc
    21181 tgatcccgtt caacgagacg cggaactacg tccagcgcgt gctcgagggc acccaggtct
    21241 atcgggtggt gctgtccgag caggaggggc gcgcccggct taggcttggc gacgacatcg
    21301 gggtcgggcc cggccggctt gccgggcgct gaggcgccgg acaggcgcgt ttccctcgac
    21361 gcggccggca aaggccggca ttctcgggcc ggggcagagt tcacggccac tctccatgcg
    21421 caacggcttg ccggacatcg aggatgtcgt cgacgccgcc cgccggctgc gcggcaaggc
    21481 ggtggtcacg ccgctgctgc gcgcgccggc gctcgaccgc atcgccggcg gcacgctcct
    21541 gatcaaggcc gagccgctgc agcgcaccgg ttcgttcaag ttccgcggcg cctacaaccg
    21601 gatcagccgc atcccggagg aagagcggag cagaggcgtg gtgtcctggt cgtcgggcaa
    21661 ccacgcccaa ggggtcgccg ccgccgccgg cctgctcggt attccggcaa ccattgtcat
    21721 gccaagcgat gcgccggcac ccaagctcga caatacccgc gccctcggcg ccgaggtggt
    21781 gctctacgac cgctatgccg agagccgcga ggacatcggg acgcggatcg ccgagcagcg
    21841 cggcgcgacc atcatcaagc cctatgacga cccctggatc atcgccggcc aaggcaccgt
    21901 cggcctcgag gccgcgggcc aggcgctggc ggccgggctg agcccggacc atgcgctggt
    21961 cgcatgcagc ggcggcggcc tggtctcggg cacggcgatc gcgctcaccg accacctgcc
    22021 cgggatcacc gtccattcgg tcgagcccga gggcttcgac gacaccgcgc gctcgctcgc
    22081 cgcgggcgca cgcctcgcca acgacccggc ggcgcgctcg atctgcgacg cattgctcgc
    22141 gccgaccccg ggcgagctca ccttcgagat caaccggcga ctgttaggcc ctggaatcgt
    22201 tgtcagcgac gcggacgcgc gcgccgccat gcgcgccgcg ttccgccacc tgaggctggt
    22261 cgccgagccg ggcggcgcgt tgccgctcgc ggccgcgctc agcggcaggt tcgactgccg
    22321 gggcaagtgc gtgctggtgg tcgtctccgg cggcaacgtc gcgcccgagc tgttcgcgga
    22381 gaccctcctc gccggcgagg acccgggtgg ctgagcctga cctggtgctc gccctcgacc
    22441 agggcaccac ctcgacccgg gcaatcgtgt tcgaccgcgc cggcaccgcg cgcgccgagg
    22501 cgcgccggcc gctgcgccag gtatatccgc gcccgggctg ggtcgagcac gaccccgagg
    22561 acatctggca agacgcggtc gcggtcatgc gcgaggcgct cgcccgcgcg cgcatcgagg
    22621 cccgcgcgct cgccgggatc ggcatcgcca accagcgcga gaccacgctc atgtggcagc
    22681 gcgacggcgg ccggccgctg cacaacgcca tcgtctggca ggaccggcgc acggccgcgg
    22741 cgtgcgaccg gctgcacgcg gccggcgccg gcgagcgcat cgcggcgctg accggcctgg
    22801 tgatcgatcc ctatttctcg gcgaccaagg cggcgtggct gctcgacacc gtcgccggtg
    22861 cgcgcgccgc ggcggagcgc ggggcgctct tgttcggcac cgtcgacagc ttcctgctgt
    22921 ggcggctgac cggcggcaag gcgcacgcca ccgacgccac caacgccgcg cgcacccagc
    22981 tgttcgacat tcaccgccgc gcctggtccg acgagctgct cgctttgttc cgcgtgccgg
    23041 gcgcggcgct gcccgaggtc aaggacagcg ccggcatgtt cggcattgcc gccgcggacg
    23101 tcctcggcgc cgaggtgcct gtgctcggca tcgccggcga ccagcaggcg gcgatggtcg
    23161 gccaggcgtg ccttgccccg ggcatggtca agagcaccta cggcaccggc ggcttcgcgc
    23221 tgctcaacac cggcacctgg gccgtggcct cgcgtgcgcg gctgttgacc accatcgcct
    23281 atcagatcgc cggcgccgcg acctatgcgc tcgagggcag catcttcgtc gccggggcgg
    23341 cgctgcaatg gctctgcgac ggcctcggcg tgctcggcga cgtcgccgac agcgaggcgc
    23401 tggcggccgg cgccgacgcg gacggtgcgg tagtcatggt acccgccttc gccggcctcg
    23461 gcgcgcccta ttgggatgcg cacgcgcgcg gcgcgctgtt cggcctgacc cgcgacgtgg
    23521 gcgtcgccga cctggcgcgg gcggcgctcg actcggtcgc tttccagacc cgcgacctga
    23581 tcgaggcgat ggcggcggac ggcgccggcc cgccgaccgc gctgcgcgtc gacggcggca
    23641 tggcggcgaa cgcgtggttc ctgcagcgcc tggccgacct gatcggggtg ccggtcgagc
    23701 ggccggtcga gaccgagacc accgccttag gcgccgcgcg gctcgccggg ctcgccgccg
    23761 gcctgatcga tgggctcgac gcgctggccg gcgaatggcg gcgcgagtgc cggttcgagc
    23821 cggcgttgga cgccgaccac cgcgaccgtc tttaccggcg ctggcaggac gcggtcgcgc
    23881 gggtgcgcga gtccgccgga ggctgagcac cgcaccattg cggtgtcaca atcctgacac
    23941 atcgtttcga taattctcga actatgaaaa ggaccgaagc cttgtcggcg ctggccgcgc
    24001 tcgcccagga cacgcggctc acggtattcc gcctgctggc acggcaggcg ccggccggcc
    24061 tgccggcggg agccatcgcc aggcgcatcg gcgccgtgcc gtcgacgctg tcgttccacc
    24121 tgagccagct cgaggccgcc gggctggtgc gctcgcgccg ccggcaacgt caggtggtct
    24181 acgccgtcga tgcggccggc atccgacggc tgctcgcgtt cctgaccgag gactgttgcg
    24241 gcgggcagcc ggagctgtgc ggcggcgtgc cggcggcgac atcgcgggag gaataccggc
    24301 cggccaaagt ggaggagaca tgatggcgga caaggtcatc aacgtcctgt tcctatgcac
    24361 aggcaactcg gcgcgcagca tcatcgccga atgcgtgatc aaccggttgg gccggccccg
    24421 cttccggggc tacagcgccg gcagccaccc gtccggcgcg atccacccgt tcgcgctcga
    24481 cctgctgcgg cgccggagtt tcccgacttc ggccctgcgg tcgaagagct gggacgagtt
    24541 cgcaagggcg ggcgcgccgg agctcgactt cgtgttcacc gtctgcgacc gcgccgcggc
    24601 cgagacctgc ccggtctggc cgggccagcc gatgagcgcc cattggggcc tgcccgaccc
    24661 ggtcgcggtg gtcggcagcg aggccgagcg gcgcttcgcc ttcgccgact cgctgcgcat
    24721 gctgatccag cggatcagca tcttcatcaa actgccgttc gaggcgctcg accggttgag
    24781 cctgaagcag cggctcgacg aaatcggcgc cgccggccgg acggcggccc aggtggagcc
    24841 gggccccggc catgactgac ttcgggctcg gccggcgcct gtgcgcggag ctcgtcggca
    24901 ccgcgatact gctggcgacc gtgatcggct cgggcatcat ggccgagcgg ctggcgggcg
    24961 gcaacgccgc cctggcgctg ctcggcaaca ccatcgcgac cggggcgatc ctgaccgtcc
    25021 tgatcctcgt gctcggcccg atctcgggcg cccatttcaa ccccgcggtc actttgtcgt
    25081 tcgtcgtcca acgccggatg acctggacag acggcgccgc gttcgccctc gcccaggtgg
    25141 cgggcggcct tgccggcatg atgctggcgc acgccatgtt cgagatgccg gtggtgatga
    25201 tcggcgccaa cgcgcgcacc ggcgccggcc agtggctggg cgaggcggtc gcgaccttcg
    25261 gcctggtggc gacgatcctc ggctgcctca gggtgcgccc cgaggcggta gcgtacgcgg
    25321 tcggcctgtt catcagcgcc ggctactggt tcacctcctc gacctcgttc gccaacccgg
    25381 cggtgaccat cgcgcgcagc ttcaccgaca gcttcaccgg catccgcccg gccgacgcgc
    25441 ccgccttcat cgccttcgag ctgatcggcg cgctcgccgc caccggcctg gtcgtctggc
    25501 tccatgcacc ccggcgcgac gccgcccgag acgagccggc ggcggtcgcg ccgccgggcg
    25561 actaggcccg cgcccgccgg ccggccccga atcccgggtg tccggggccg gcgaaagccc
    25621 ctcggcggcg ccgcatggcc tgggggaatc ccctgcccgc tctcggggcg gagtgggttt
    25681 ccgcggccac acagcacctt atcgcccgct catcatgccg gcgtagtgca tttgcatcat
    25741 gactcttcca ggtcgcttgg cttttaataa aggttgagcc atccggcctg cacggggcct
    25801 gagggcggtc gcgcatgtgc cagggccggg cccggccgct accgggcaag cgagaggttt
    25861 cggggggcta aggctttggt ctggcaatcg ggacgcatgg atgtggatgc cgcgacggcg
    25921 gcggccgtcc cggacatcgt tgccggcgca acgcgcatcc tcgagatcgg gcccgacaac
    25981 gtcgtccggc ttgccgacgg ccgcatcatc accgacgcgg tctatttgcg cgcgggcgac
    26041 gacctggtgc tgcgcgtccc agacggcgcg acctacgtca tcaagggcta cttcgccctc
    26101 accgatcgcc cggacctggt gagcgatgac ggcggccgca tgactcccgg catggtcgat
    26161 gcgttcaccc tcaaccacca gcccgagcag gtcgcccagg caagcgaggc gttggccgcg
    26221 ggcgcggtgg cgacggtggc ggtgctggaa ggctcggcca ccgtcacgcg cgccgacggc
    26281 agcgtggtcc cgctcagcga aggcgcgccg atctatcagt tcgacatcat cgagtccggc
    26341 gccgacggcg cggtcaggct gctgttcatc gacaacaccg tgttcgcgat cggcccaggc
    26401 gcgcggctca ccatcgacga cttcgtgttc gacacggccg gcgacggcag cgtctcggcg
    26461 ttcaccatgc tcaagggcat gttcgctttc gtctccggcc agatcgccga caacgacccg
    26521 agcgacttcg tgctcaccac gccggtggcg gtggtcggca ttcgcggctc ggccgggctc
    26581 ggcgacgtca atgagccggg aacgccgagc cggttcaccg tcgacgagcc gacgctcgtg
    26641 gtgaccaaca atggcggcag cgtcatcctc gaccagcggt tcgcgaccac caacgtcacc
    26701 gatatcaaca ccccgccgag cgcgcccgag atcctcgacg aggacgaggt caagacgatc
    26761 tatggaccga tgggaggtgt cgggccgtcc ttcctgtcgg acgaatcgaa cggccaggac
    26821 gagcagtcgg cgccgcaggg accgggcgat cagggcgccg cgcccggcgg cgtgcccgac
    26881 gtggtgaccg cggcgggccc cgcctcgggc ggccaatccg ggcctccgcc cagcgacggc
    26941 ggcacctcgc agttcaccga gatggactcg gaccaggtgc tgatccacga cctcggccga
    27001 gccggcacca cgccgaccgc caccggcggc accgccggca gctccggcgg catcggcgga
    27061 ggcgccgagc cgagccccgg cccgacctcg cccagcaacc agccagcgac cggcggcggc
    27121 agccagccgc cccccgacca gggcggatcg ccgccgtccg aaccgccggg cgagacgccg
    27181 ctgttctggg tcggcggccc gggcgacgat ttccaggtcg gcaccagcct tgccgactcg
    27241 ctcgacggtg ccggcggcaa cgacaccctg ctgggtctcg gcggcaacga cacgctgatc
    27301 ggcggcaccg gcaacgactc cctcgttggc gacgacggcg acgacagcgt cacgggcggc
    27361 gacggcgacg acaccatcgt cgccggcagc ggccagggta acgacaccta tctcggcggc
    27421 ggcggcgacg acacgctcga ctactcgagc gcggtgactc cgctgtcgat cgacatgggc
    27481 gaaggcacca cggtcggcgg cccggagatc ggcaacgacg tcttcgacgg catcgagcac
    27541 gtgatcgccg gcgccggcga cgacacgatt gccggcgaca gcaacgccaa catgatcgac
    27601 ggcggcggcg gcgcggaccg gctcgacggc ggcggcagca gcgacaccct gctcggcggc
    27661 gacggcgacg acgtcctgac ttacgacccc gccgacggcc aggtcgacgg cggcggcggc
    27721 ttcgacacgc tgcgcccgcc cgaaggcagc gtgatcgatc tcaccgccga ggtccgcccg
    27781 gacctgtccg ggatcgaggc gatcgacctc accgacctcg acgccgacac cttgatcatc
    27841 gacggcgcga gcctgctcga cctggtcggc ccgggaggtg cgcttcgggt gctcggcacc
    27901 gcccaagact cggtgattgc cggcggcggc tggaccgaga acggcgtcca ggcgatcgac
    27961 ggcgagacct atcggacctt tacccagaac ggcgcgaccc tgctggtcga caccgacatc
    28021 gaccgcgccg atatcgagcc gttcgcctcg ccgccgacgc tttcggtcca gccggcccag
    28081 gccaatgaga accagccggt gccgctggtg atcgacgccg cgctggtcga caccgacggc
    28141 tccgagacgc tcgccatcca gatcgagaac gtacccgcag gcgcggccct gtcggcgggc
    28201 gcggaccagg gcgacggcat ctggttgctg gcgccgggcg atctcgccgg cctcacgctc
    28261 gatctgcccc cggacttcga cgcggcgttc gaccttcagg tgaccgcgat cgccaccgag
    28321 accgaggacg ggtcatttgc cgagaccagc gcgaacctga cggtgacccc gacccccgac
    28381 ctctcgatcg ccgatgccat cgtcgtcgag ccggcgttcg gggcggtggt gcaggtccaa
    28441 ggcatcggcg ccaacggcgg cacgttggtc gacgggctcg gcggccccgc cggcttcggc
    28501 gagaacacct tccccgccaa cgacgacgac tcggtgttcg tcgacgtgac cgcggtgttc
    28561 ggcgccggcg gcatgaactt cttcggcacc aacttcaccg gcttctggct caacaacaac
    28621 ggcaacatca cgttcaacga gccgcaggcc gagttcacgc cgattgccat taccggcgac
    28681 acgggcaacc cgatcatcgc gcccttcttc gccgacatcg ataccctcgg ctcgcacgaa
    28741 aacgcgccca cgcccggcgg caactcgacc ggctcgaacc tggtctactg ggacatcgac
    28801 gccgccaacg gcgccatcac catcacctgg gacgacgtcg gctactacgc cggccacacg
    28861 gacaagctga acgcgttcca gctggtcatc tacaaggtca atgacgccgg cgatttcgcg
    28921 ttcgagttcc gctacgagac catcgactgg acgaccggcg atgccagcgg cggcgaaggc
    28981 ggcctcggcg gcgtcgtcgc gcgcgccggc tggtccgccg gcgacggcgt caacttcctc
    29041 gagctgccgc agtcgggcaa cgaggcggcg gtgctcgacc tcgagaacac cagcaacgac
    29101 ggcaccgcgc cggacggcaa ctgggtgttc ctcgccgagg gcggcgatgt cggcaacctg
    29161 ccgagcggcg tcgcgctggt cgacgtggtg ctcgatgcgc cgagcaacga tgtggtcacc
    29221 gtcgactacc agaccgtcga cggcaccgcc accagcaccg gcacgctggg cgacggcgac
    29281 ttcgactacc agccggtcgc gggctcgctg gtgttccagc cgggcgagac ggtccagacg
    29341 atcgcgatca ccatctacgg cgacaacgcg gtcgagccga acgaggtctt caccgtcgag
    29401 ctgagcaatg caacgaatgc cggcatcatc gatgcgcagg cgaccgttac catgaccgac
    29461 agcacggcgc cgccggcgcc ggtcgacgac gcccggtcgg cgttcgatgc cgacctggtc
    29521 ggcagcgacg gagccgtcca gtacgccggc aacctgctcg acaacgacga gctcggcaac
    29581 gccctgctcg ccgcccagcc ggtggtcgac atcgccgtgc ttaccgccta tccaggtggt
    29641 gtctcggtcg acgacagcgg gccatatgtc gcctcgagca acgacggcgc ctggatgctc
    29701 acggtcgacc ccgagaccgg cgactacacg ttcacgctca accaggcgct cgatcatcag
    29761 gcgttcggcg acggcgacgt catcgatctc gccttctcct atcggatcga gaccgacacc
    29821 ggcttccagt cggacccggc aacgttgacc gtctcggtgt tcgacgacgc gccggtcgcc
    29881 agccaggacg ccgacctcgc ggtgctgacc accgacggcg agatcgcggg ggtcaacctg
    29941 ctggccaatg acgaggtcgg tggcgacggc ggcgagatca ccgcgatcac ctatgacgac
    30001 gacgggccgg tgacggtgcc gttggtcgag ggctcggtca cagtgctgac agcgctcggc
    30061 ggcacgctca ccgtcaacgc cgacggctcg tggagctacc agccggcccg gctgatcggc
    30121 gcgccgcaag cgaccgacag cttcacctac accttgaccg acaacgacga cgacggcgcc
    30181 caggcgatcc agaacgtcac cgtcaccgag gtgccgctgg tcgcgccggt ggtcgacctc
    30241 gacgggcccg gcggcggcac cgggttcgat gccggcacct tcgtcgaggg cggcgcgccg
    30301 cgggcggtgg tcgatgccga cgccacgatc agcgacgtcg acagcgccaa cctgctgtcg
    30361 ctgacggcga ccttgaccaa cccgctcgac ggccctgacg aggcgctcgc cgccgacatc
    30421 tcgggcactg cgatcgtcgc caactatgtc agcgagaccg gcgtgctgac gctgtccggc
    30481 ctggactcgg tcgccaacta ccaggcagtg ctgcgcacgc tcacctacca gaacacatcc
    30541 gagagccccg acccgaccca gcgggtgatc gaggtccagg ccgaggacga ggacgagcag
    30601 ctctcgaatg ttgccgcggc gaccattgcc ctgctggcgg tgaacgataa tccggtcgct
    30661 gtcaccgacg cctatgcggc cgatgaggac agcatcctca ccgtcaatgc ggccggcggc
    30721 gtgatcgaca acccggtgac gttcgactcg gacccggacg gggatgcgct gtcggtcgtg
    30781 gcgttcgatg ccgtgagcac gcttggagcg acggtggtca tgcaggccga cggctcgttc
    30841 acttacgacc cgaccggggt tgccgcgctc cagcagctcg gcgccggcga ggacacggtc
    30901 gacagcttca cctacaccat cggcgacggc aacggcggcc aggacaccgc ctcggtcgaa
    30961 gtcacggtga gcggcgcgga cgaggtcgcg accgagggca acga
//
LOCUS       DASWHU010000385         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_639718, whole genome shotgun sequence.
ACCESSION   DASWHU010000385 DASWHU010000000
VERSION     DASWHU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639718"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGB88_14350"
     CDS             complement(<1..353)
                     /locus_tag="VGB88_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648684.1"
                     /translation="MLIWTPAETERGGGSGPRGPKRADEAFTEASAGGGAPRPATGTD
                     DVLLLPSLVRETPLDLIDEGAWCAADHAVGTPGRRKVARADWIEESARRILIDAAYGR
                     EIEWLADMGNDSAKSA"
     gene            606..2069
                     /gene="guaB"
                     /locus_tag="VGB88_14355"
     CDS             606..2069
                     /gene="guaB"
                     /locus_tag="VGB88_14355"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9648685.1"
                     /translation="MDLFEALTFDDVLIKPAASAVLPAQVDTATRFTRTISLGIPLAS
                     AAMDTVTESALAIAMAQAGGIGVIHRNLDAAGQADEVKRVKKFESGMVVNPVTVTPDM
                     TLDDLHRLQDRHNISGFPVVEAGNGRLVGIVTRRDVRFATNGRTPVRELMTTEGLVTV
                     KQGIGGEAARKLLHEHRIEKLLVVDDGYHCVGLITYTDIEKARAFPNACKDDKGRLRV
                     AAAIGVGEAGIARAEALIDAEVDVIVVDTAHGHAQAVIDAVGQVKKLTNYTQIIGGNV
                     ATAAGAKALIDAGADAVKVGIGPGSICTTRIVAGVGMPQFSALQETASVCRRADVPVI
                     GDGGIRFSGDLAKAIAAGAECAMIGSLLAGTEESPGEIYLYQGRSYKAYRGMGSIGAM
                     ARGSADRYFQQEVADSLKLVPEGVEGRVPFKGPAVTVVHQLIGGLRAAMGYTGTRTIR
                     EFQDRAEFVRVTGAGVREGHVHDVTVTREAPNYNPGE"
     gene            2127..>2815
                     /locus_tag="VGB88_14360"
     CDS             2127..>2815
                     /locus_tag="VGB88_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methylase"
                     /protein_id="HEX9648686.1"
                     /translation="MTPAARLQAAIELSDAVEQSAAPADATVRAALRRRRYVGAKDRR
                     EILELVFDAARRRVRLDWRIARHGGAASARLRLLALAAVERGQGVAAVESLCDGGRYA
                     PAPLDDGERALLAAMAGSAARDDADMPPWAQAGVPEWLAPSLIRRFGDALAAEMAALA
                     EPAPLDVRVDTRRATRAAVLAELRSFGLDAEPTPLSPVGIRVRGRPLLSRHPAWREGV
                     IEVQDEGAQLVA"
BASE COUNT          386 a         1015 c         1038 g          376 t
ORIGIN      
        1 gcgctcttgg cggagtcgtt gcccatgtcg gcgagccatt cgatctcgcg cccgtaggcg
       61 gcgtcgatca ggattctgcg cgcgctctcc tcgatccagt cagcgcgcgc gaccttgcgg
      121 cggcccgggg tgccgacggc gtgatcggcc gcgcaccacg cgccttcgtc gatcaggtcg
      181 agcggcgtct cgcgcaccag gctcggcagc agcagcacgt cgtcggtgcc ggtggcaggg
      241 cgcggcgcgc cgccgccggc gctggcctcg gtgaacgcct cgtcggcacg cttggggccg
      301 cgtggccccg agccgccgcc gcgttcggtc tcagcaggtg tccagatcag catgtccgcc
      361 tccggcgttc ggcgcccggc ccgcagccgg acgaacaaga ccgtcaaggc gaatgtgcat
      421 cgagcctgcc tccggacgcg gtgagaagga tcacaaggcg tcacgctttc ggtgtgaccg
      481 cggtcacaat ctcctttgat tttgacggta ataaggcgtc ggcgtgactg ttcgcgcacc
      541 gtgattccgg cttggcgcaa accggcgaaa gggtatagtg gcgcaccccg cgacctggag
      601 gcggcatgga cctgttcgag gcgctgacct tcgacgacgt actgatcaag ccggcggcgt
      661 cggccgtgct gcccgcccaa gtcgataccg cgacgcgctt cacccgcacc atctcgctcg
      721 gcatcccgct cgcctcggcg gccatggaca cggtgactga atcggcgctc gccatcgcca
      781 tggcgcaggc cgggggcatc ggcgtgatcc accgcaacct cgacgccgcg ggccaggccg
      841 acgaggtcaa gcgggtgaag aagttcgagt ccgggatggt ggtcaatccg gtgaccgtga
      901 ccccggacat gacgctcgac gacctgcatc ggctgcagga ccgccacaac atctccggct
      961 tccccgtggt cgaggcgggc aacggccgcc tggtcggcat cgttacccgc cgcgacgtgc
     1021 gcttcgccac caacgggcgc acgccggtgc gcgagctgat gaccaccgaa ggcctggtca
     1081 cggtcaagca ggggatcggc ggcgaggcgg cccggaagct gctgcacgag caccggatcg
     1141 agaagctgct ggtggtcgac gacggctatc actgcgtcgg cctgatcacc tataccgaca
     1201 tcgagaaggc gcgcgccttc cccaacgcct gcaaggacga caagggccgg ctgcgggtgg
     1261 cggcggcgat cggcgtcggc gaggcgggca tcgcccgcgc cgaggcgctg atcgacgccg
     1321 aggtcgacgt gatcgtggtc gacaccgccc acggccacgc ccaggccgtg atcgacgcgg
     1381 tcggccaggt caagaagctc acgaactaca cccagatcat cggcggcaac gtcgccaccg
     1441 ccgccggcgc caaggcgctg atcgacgccg gggcggacgc ggtcaaggtc ggcatcgggc
     1501 cgggctcgat ctgcaccacg cgcatcgtcg caggcgtcgg catgccgcag ttctcggcgc
     1561 tgcaggagac cgcctcggtc tgccgccgcg ccgacgtgcc ggtgatcggc gacggcggca
     1621 tccgcttctc cggcgacctc gctaaggcga tcgcggccgg cgccgaatgc gccatgatcg
     1681 gctcgctgct cgccggcacc gaggagagcc cgggcgagat ctacctgtat cagggccgca
     1741 gctacaaggc gtaccgcggc atgggctcga tcggcgccat ggcgcgcggc tcggccgacc
     1801 gctacttcca gcaggaggtg gcggactcgc tcaagctggt gccggaaggg gtcgagggcc
     1861 gggtgccgtt caagggcccg gcggtgaccg tcgtccatca gctgatcggc ggcctgcgcg
     1921 cggcgatggg ctataccggc acccgcacca tccgcgagtt ccaggaccgg gccgagttcg
     1981 tgcgcgtcac cggcgcgggc gtgcgcgaag gccacgtcca cgacgtcacc gtcacccgcg
     2041 aggcgcccaa ctacaatccc ggcgagtgac cgccggccgc gccggcgccg cctgacgccg
     2101 gcgccgctgg ccgcatcccc cgagcgatga ccccggcggc gcgcctgcag gccgcgatcg
     2161 agctctcgga cgcggtcgag cagagcgcgg cgccggccga cgcgacggtc cgcgccgcgc
     2221 tccgccggcg gcgctatgtc ggcgccaagg accggcgcga gatcctggag ctggtgttcg
     2281 atgccgcccg ccggcgggtc cggctcgact ggcgcatcgc ccgccacggc ggtgcggcga
     2341 gcgcgcggct caggctgctg gcgctggcgg cggtggagcg cggccagggc gtcgccgcgg
     2401 tcgagtcgct gtgcgacggc ggccgctacg cgccggcgcc gctcgacgac ggcgagcgcg
     2461 cgctgctcgc ggcgatggcc gggtcagcgg cccgcgacga cgcggacatg ccgccctggg
     2521 cgcaggcggg cgtgccggag tggctggcac cgtcgctcat ccgccgcttc ggcgacgcgc
     2581 ttgccgccga gatggcggcg ctcgccgagc cggcgccgct cgatgtgcga gtggacacgc
     2641 gccgcgcgac gcgcgcggcg gtgctcgccg agctccgctc gttcggcctc gatgccgagc
     2701 cgacgccgct gtcgccggtc ggcatccgcg tgcgcggccg gccgctgttg agccgccacc
     2761 cggcgtggcg cgagggcgtg atcgaggtcc aggacgaggg cgcgcagctg gtcgc
//
LOCUS       DASWHU010000386         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3573340, whole genome shotgun sequence.
ACCESSION   DASWHU010000386 DASWHU010000000
VERSION     DASWHU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573340"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VGB88_14365"
     CDS             complement(<1..789)
                     /locus_tag="VGB88_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9648687.1"
                     /translation="MTQFGVSQPVRRTEDVRFVTGRGRFVDDIDPPGQAHGVVVRAPH
                     AHALIRMIDVSAAAAAPGVVAVITGAELDEAGANALPCLAPVGNSIDVPRPVLCTKRV
                     CHVGDAVAFVVADTLAHAKDAAELVAVDYRPLAAVVDTATAHARGQPQIHDRAKGNRC
                     FDWRHGDARAVKKAFSAAAHVVGLDIVNNRLIGHAMETRGALAEPGPGKDRMTLTVCT
                     QGGWLVRDVIAESVLKVAPESLRVITPDVGGGFGIKIFPYPEYAL"
     gene            1140..2189
                     /locus_tag="VGB88_14370"
     CDS             1140..2189
                     /locus_tag="VGB88_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX9648688.1"
                     /translation="MYSQKLVAYGQRLKAWNGETPAPRGGEVLVRVRNAGVCHSDVHL
                     QDGYFDLGGGERLDIASGRALPFTLGHEVEGEVAALGPDARGVRLGERVVVYPWIGCD
                     GCALCARGDGHLCAKPRQIGVTIDGGYADHCLVPDAKYLFPYGAVEPALAATYACSGI
                     TAYGALAKAGGAAGDGPVLIVGLGGVGMAALSFARALYKTAPLVADIDPAKREAALAA
                     GAGAAFDPADKGAAKAVRAATGGGVSAALDFVGADSSFAFASAALGRHGTLVVVGLLG
                     GRMTLPLAMFPLKGITVTGSYVGSLGQFRAMMALVRDGRVAPIPIQTRPLADASRTLD
                     DLRAGRVLGRAVLVP"
BASE COUNT          304 a          815 c          835 g          305 t
ORIGIN      
        1 cagcgcgtat tcggggtagg gaaagatctt gatgccgaag ccgccgccga cgtcgggggt
       61 gatcacgcgc aagctttcgg gcgcgacctt gagcacgctc tcggcgatca cgtcgcgcac
      121 cagccagccg ccctgggtgc acacggtgag cgtcatccgg tccttgcccg ggcccggctc
      181 ggcgagcgcg ccgcgggtct ccatcgcgtg cccgattagg cggttgttga cgatgtcgag
      241 gccgaccaca tgggccgcag ccgaaaacgc cttctttacc gcgcgcgcat cgccgtgccg
      301 ccagtcgaag caccggttgc ccttcgcccg gtcgtgaatc tgcggctggc cgcgcgcatg
      361 ggcggtagcg gtatcgacca ccgccgcaag cggccggtaa tcgaccgcga ccagctcggc
      421 cgcgtctttc gcgtgggcga gcgtgtcggc gaccacgaac gcgaccgcgt cgccgacatg
      481 gcagacgcgc ttcgtgcaca gcaccggccg cggcacatcg atgctgttgc cgaccggggc
      541 gaggcacggc aacgcgttgg cgccggcctc gtcgagctcg gcgccggtaa tcaccgcgac
      601 caccccgggc gccgccgccg cggccgacac gtcgatcatg cggatcaggg cgtgggcgtg
      661 cggcgcgcgc accaccacgc cgtgcgcctg gcccggcgga tcgatgtcgt ccacgaaccg
      721 gccgcgcccg gtgacgaagc gcacatcctc ggtccgccgc accggctggc tgacgccgaa
      781 ctgggtcatg cctcccctcc cgccaagcgc aaatcgggcg acgttagcgg cagcggccgt
      841 ccggcggcaa ccgagaccga atgaaaagcc tagtcggccg gccgccatca ggttcacgcg
      901 gaggcgcgga ggccgcagaa gcccccctcc ttcctccccc cgcaagcagg gggaggcaat
      961 aaaataaaga ccctcccccg cttgcggagg agggctggga gggggtgcgg cgggcgccag
     1021 cccaccctcg aaatcctctg tggcacccgc ggctccgcgt ctccgcgtgc cgaacaccgc
     1081 cgcatgggac aagccgcggc ggcgaaccta tagtggcgcg gcgcagcagg gggatgtcga
     1141 tgtacagcca gaagctcgtc gcctatgggc agcggcttaa ggcctggaac ggcgagacgc
     1201 ccgcaccgcg aggcggcgag gtgctggtgc gggtgcgcaa cgctggcgtc tgccacagcg
     1261 acgtgcacct gcaggacggc tacttcgacc tcggcggcgg cgagcgcctc gacatcgcct
     1321 cgggccgggc gctgccgttc accctcggcc acgaggtcga gggcgaggtc gcggcgctgg
     1381 ggcccgacgc gcgcggggtg cggctcggcg agcgggtggt ggtctatccg tggatcggct
     1441 gcgacgggtg tgcgctgtgc gcacgcggcg acggccatct gtgcgcgaag ccgcgccaga
     1501 tcggggtcac catcgacggc ggctatgccg accactgcct ggtgcccgac gccaagtact
     1561 tgttcccgta tggcgcagtc gagccggcgc tcgccgcgac ctacgcctgc tcgggcatca
     1621 cggcctacgg cgcgctggcc aaggccggcg gagcggccgg cgacgggccg gtgctgatcg
     1681 tcggcttggg cggcgtcggc atggcggcgc tgtcgttcgc ccgcgccttg tacaagaccg
     1741 cgcccctggt cgccgacatc gacccggcga agcgcgaggc ggcgctcgcc gccggtgccg
     1801 gggcggcgtt cgatccggcg gacaagggcg ccgccaaggc ggtgcgcgcc gcaaccggtg
     1861 ggggcgtcag cgcggcgctc gacttcgtcg gcgcggacag ctcgttcgcc ttcgccagcg
     1921 ccgcgctcgg gcgccacggc acgctggtcg tggtcgggct gctcggtggg cgcatgaccc
     1981 tgccgctcgc gatgttcccg ctgaaaggca taacggtgac cggctcctat gtcggctcgc
     2041 tcggccagtt ccgcgcgatg atggcgctgg tgcgcgacgg gcgggtggcg ccgatcccga
     2101 tccagacccg cccgctcgcc gatgcctcgc gcaccctcga cgacctgcgc gcggggcgcg
     2161 tgctggggcg cgcggtgctg gtgccgtgac cgcgcgcgcc gcaccaacca gccgggtcgc
     2221 gctggtcacc ggtgccgcgc gcggcatcgg cgcggcgat
//
LOCUS       DASWHU010000387         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3235500, whole genome shotgun sequence.
ACCESSION   DASWHU010000387 DASWHU010000000
VERSION     DASWHU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235500"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..>1938
                     /locus_tag="VGB88_14375"
     CDS             92..>1938
                     /locus_tag="VGB88_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9648689.1"
                     /translation="MAERARVVVIGAGIVGSALAYHLARRGRDGIVVLDRGPLFDTGG
                     STGHAPGLVFQTNASRLLTRLARMSVRLYRALRLDGAPGWRAVGGIEVATTDARWADL
                     HRKLGFARSWGVGGAALLTPAEVAARLPLVDRGRILGGLWVPGDGVADGARLCAALAR
                     RAEARGAARFRGETEVTGFDVANGRLRAVLTDRGAIETEVAVICAGLWSPGIGKLAGI
                     SVPLVAVEHQLARTTTVPALAGTGEDEIAHPILRHQDQDLYVRQAGDRYVIGSYRHAP
                     RLVEADDLGATALRPFTAADFAPAFAEAKALLPPLADAHVEDGINGMFSFTPDGLPLL
                     GPTEVDGLWLGAAVWVTHAGGAARALADWLVDGEPRIDLHAADANRFHAHQRTPAYVR
                     TRGAQQYVEVYDVIHPRQQIAAPRGLRLSPFQARFEALRPEMVEVAGWERPLWFAANE
                     GLLDRFAVAGRNGWAARHWSPVEGAEHLATRAGVALFDLSPLVKIEVSGPGAALLLQW
                     LCANQIDRPPGAVVYTAMLGWRGGIMADLTVTRLAADRFQIVTGAASGMHDLAWIRGH
                     AAGDDTVRVAELTSGLAALGLWGPKARAVLAAACPDDDVADAAFPYLAAR"
BASE COUNT          221 a          724 c          721 g          272 t
ORIGIN      
        1 gagcggcacc ttgagccccg ccgccgccgc gacccgcctg ctcgacgcag tggtcggcgc
       61 ggcgtcaaag tcggattgat cggcgcgcgc catggccgag cgcgcgcggg tggtggtgat
      121 cggcgccggc atcgtcggct cagcgctcgc ctaccacctc gcccggcgtg gccgcgacgg
      181 catcgtcgtg ctcgaccgcg ggccgctgtt cgacaccggc ggctccaccg gccacgctcc
      241 cgggctggtg ttccagacca atgcctcgcg cctgctcacc cgactcgcgc ggatgagcgt
      301 ccgcctctat cgcgcgctca ggctcgacgg cgcgcccggc tggcgcgcgg tcggcggcat
      361 cgaggtcgcg accaccgacg cgcgttgggc agaccttcac cgcaagcttg gctttgcccg
      421 gagttggggg gtaggcgggg ctgcgttgct cacgccggcc gaggtcgcag cgcgactccc
      481 gctcgtcgat cgcgggcgca tcctgggcgg gctgtgggtg ccgggcgacg gcgtcgccga
      541 cggcgcgcgc ctgtgcgccg cgctcgcccg ccgggcggaa gcgcggggtg ccgcgcgctt
      601 ccggggcgag accgaggtca ccggcttcga cgtagcgaac ggccggctgc gcgcggtact
      661 gaccgaccgc ggcgcgatcg agaccgaggt cgcggtgatc tgcgccggcc tgtggtcgcc
      721 ggggatcggg aagctcgccg ggatttccgt gccgctggtc gcggtcgagc accagctcgc
      781 ccgcaccact acggtgcccg cgctcgccgg gacgggagag gacgagatcg cccacccgat
      841 cctgcgccac caggaccagg acctctacgt ccgccaggcc ggcgaccgct atgtcatcgg
      901 cagctaccgc cacgccccgc gcctggtcga ggccgacgac ctgggcgcga cggcgctgcg
      961 cccgttcacg gcggccgatt tcgcgcccgc cttcgccgag gccaaggcgc tgctgccgcc
     1021 gctcgccgac gcgcacgtcg aggatgggat caacggcatg ttctcgttca cgcccgacgg
     1081 cctgccgctg ctcgggccga cagaggtcga cgggctgtgg ctcggcgcgg cggtgtgggt
     1141 gacgcatgcc ggcggcgctg cgcgcgcgct cgccgactgg ctggtcgacg gcgagccgcg
     1201 catcgacctg cacgccgccg acgccaatcg tttccatgct catcaacgga cgcccgccta
     1261 tgtgcgcacg cgcggcgccc agcaatatgt cgaggtctac gacgtcatcc atccgcgcca
     1321 gcagatcgcc gcgccgcgcg ggctcaggct gagcccgttc caggcgcgat tcgaggcgct
     1381 gcgtcccgag atggtcgagg tggcgggatg ggagcgcccg ctttggttcg ccgccaacga
     1441 ggggctgctc gaccgcttcg ccgtggccgg gcgcaacggc tgggcggcgc gccactggtc
     1501 gccggtcgag ggcgccgagc acctggcgac gcgcgccggc gtggcgctgt tcgacctgtc
     1561 gccgttggtc aagatcgagg tctcgggtcc cggcgcggcg ctgcttctgc agtggctgtg
     1621 cgccaaccag atcgaccggc cgccgggggc ggtggtctac accgcgatgc tggggtggcg
     1681 cggcggcatc atggccgacc taaccgtcac ccggctcgcc gccgaccggt tccagatcgt
     1741 caccggcgcc gcctcgggca tgcacgacct cgcctggatt cgcggccacg ccgccggcga
     1801 cgacacggtg cgggtcgccg agctgacctc gggcctcgcc gcgctcggcc tgtgggggcc
     1861 gaaggcgcgc gccgtcctcg ccgcagcgtg cccggacgac gacgtggcgg atgccgcctt
     1921 tccctatctc gcggcgcg
//
LOCUS       DASWHU010000388         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2871408, whole genome shotgun sequence.
ACCESSION   DASWHU010000388 DASWHU010000000
VERSION     DASWHU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871408"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="hslU"
                     /locus_tag="VGB88_14380"
     CDS             complement(<1..920)
                     /gene="hslU"
                     /locus_tag="VGB88_14380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX9648690.1"
                     /translation="MTEFSPREIVSELDRFIVGQDQAKRSVAIALRNRWRRMQLTGDL
                     RDEVLPKNILMIGPTGVGKTEIARRLARLAGAPFVKVEATKFTEVGYVGRDVESIVRD
                     LVEVSINMTRERLRASVRARAEAAAEERVLDALVGAKAAPETRQKFRKLLREGTLAER
                     EIELEVQDSGFPGIPSFDLPGVPGAQMSMINLNEMLGKAMGGRTRRRKMRVDETYEVL
                     MAEESDKLLDDEKVVREALAAVEENGIVFLDEIDKICARAGERFGADVSREGVQRDLL
                     PLIEGTVVATKHGQVRTDHILFIASGAFHLA"
     gene            complement(917..1471)
                     /gene="hslV"
                     /locus_tag="VGB88_14385"
     CDS             complement(917..1471)
                     /gene="hslV"
                     /locus_tag="VGB88_14385"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9648691.1"
                     /translation="MTSRTKAGWRGTTVLSVRKGRQVVVAGDGQVTFAETVIKSNARK
                     VRRLGEGKILAGFAGATADAFTLFERLEAKLEQHPGQLARACVELAKDWRTDRYLRRL
                     EAMMAVADADISLVLTGTGDVLEPEDGLIGIGSGGTYALAAARALIDIDGLDAAQIAT
                     KAMGIAADICVYTNRELVIETLER"
BASE COUNT          209 a          550 c          545 g          250 t
ORIGIN      
        1 gccaggtgga acgcgcccga ggcgatgaac aggatgtggt cggtgcgcac ctggccgtgc
       61 ttggtcgcga ccacggtgcc ctcgatcagc ggcagaaggt cgcgctggac gccctcgcgg
      121 ctcacgtcgg cgccgaagcg ctcgccggcg cgggcgcaga tcttgtcgat ctcgtcgagg
      181 aacacgatgc cgttctcctc gaccgcggcc agcgcctcgc gcacgacctt ctcgtcgtcg
      241 aggagcttgt cgctctcctc ggccatcagc acctcgtagg tctcgtcgac gcgcatcttg
      301 cgccggcgcg tgcgcccgcc catcgccttg ccgagcatct cgttgaggtt gatcatgctc
      361 atctgggcgc cgggcacgcc gggcaagtcg aagctcggga tgccggggaa gccgctgtcc
      421 tggacctcga gctcgatctc gcgctcggcg agcgtgccct cgcgcaggag cttgcggaac
      481 ttctggcgcg tctcgggcgc ggccttcgcg ccgaccagcg catcgagcac gcgctcctcg
      541 gccgcggcct cggcgcgcgc acgcacgctc gcgcgcagcc gctcgcgggt catgttgatc
      601 gacacctcga ccaggtcgcg gacgatcgac tcgacgtcgc gcccgacata gccgacctcg
      661 gtgaacttgg tcgcctcgac cttgacgaac ggggcgccgg cgaggcgcgc gaggcggcgc
      721 gcgatctcgg tcttgccgac cccggtcggg ccgatcatca ggatgttctt gggcagcacc
      781 tcgtcgcgca ggtcgccggt gagctgcatc cgccgccagc ggttgcgcag cgcgatcgcc
      841 accgagcgct tggcctggtc ctggccgacg atgaagcggt cgagctcgga gacgatctcg
      901 cgcgggctga actcggtcat ctctccaagg tctcgatcac cagctcgcgg ttggtgtaga
      961 cgcagatgtc ggcggcgatc cccattgcct tggtcgcgat ctgggccgcg tcgaggccgt
     1021 cgatgtcgat cagcgcgcgc gccgcggcga gcgcgtaggt gccgcccgag ccgatgccga
     1081 tcagcccgtc ctcgggctcg agcacgtcgc cggtgccggt cagcaccagc gagatgtcgg
     1141 cgtcggcgac cgccatcatc gcctcgagcc ggcgcaaata gcggtcggtg cgccagtcct
     1201 tggcgagctc gacgcaggcg cgggcgagct ggcccggatg ctgctcgagc ttggcctcga
     1261 ggcgctcgaa cagggtgaac gcgtccgccg tcgcgccggc gaatccggcc aggatcttgc
     1321 cctcgccgag gcggcgcacc ttgcgcgcgt tcgacttgat cacggtctcg gcgaaggtca
     1381 cctggccgtc gccggcgacc accacctgcc tgcccttgcg caccgacagc acggtggtgc
     1441 cgcgccagcc ggccttggtc cgagatgtca tgagatggcc aggatcggga cggttccggt
     1501 gaaaggcagc ccctaacgtg gcgatatcgc cgccccggtc aaggggtggg gccg
//
LOCUS       DASWHU010000389        13013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1020846, whole genome shotgun sequence.
ACCESSION   DASWHU010000389 DASWHU010000000
VERSION     DASWHU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020846"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..276)
                     /locus_tag="VGB88_14390"
                     /pseudo
     CDS             complement(100..276)
                     /locus_tag="VGB88_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(324..1172)
                     /locus_tag="VGB88_14395"
     CDS             complement(324..1172)
                     /locus_tag="VGB88_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9648692.1"
                     /translation="MTSTGRRILAAVLRLVSWNVRRTVDAWRELARDGALDIALLQEA
                     VPPPPDMAVETVPSGSESWSTVGGRRLFCAAVAKFGNRIRFTPITARSLLEANDREMA
                     VSRPGSIAAADVELANGERLTLVSLYAAWERPREGEWIYADASAHRLVSDLSVFIGSQ
                     RGHRIIAAGDLNILMGHGEDGSPYWRDRYLSIFSRMASIGLPFVGPQHPHGIQAEPWP
                     LELPRESKNVPTFRVRQADPATATRQLDFVFASAELHHRLRVRALNSLDEWGPSDHCR
                     VLVELS"
     gene            1211..1456
                     /locus_tag="VGB88_14400"
     CDS             1211..1456
                     /locus_tag="VGB88_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648693.1"
                     /translation="MTHEVGDRGIAKRVGKIGLGAVELSAATNQAAELAVVDVVEVVC
                     KPERLDDVGAGCDAGAAAAGEVPAVIRGTARRPGACR"
     gene            complement(1841..2647)
                     /locus_tag="VGB88_14405"
     CDS             complement(1841..2647)
                     /locus_tag="VGB88_14405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9648694.1"
                     /translation="MAGRLQDKVAIVTGAGSSGPGWGNGKATAVLFAREGARLFAVDV
                     NRDALAETAAIVEGEGCPCTSHVCDVSSPGAVADMVEACIDTFGRIDVLHNNVGIVDP
                     GTPEDIGENAWDRLIDVNVKSMYLTCRHVLPHMVAQGGGAIVNLSSIASFFSLGYPCA
                     SYAASKGAVNALTRNIASHYARHKVRCNATLPGLMDTPLIRGAVAAAYDDVAAMIARR
                     NAQCPMGFMGDAWDTARAALFLASDEARYITGIELVVDGGLTITMSPAQG"
     gene            complement(2699..3025)
                     /locus_tag="VGB88_14410"
     CDS             complement(2699..3025)
                     /locus_tag="VGB88_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648695.1"
                     /translation="MHIRCAYFEGEVAPEMRARFDRFMDTEIMPRLTSMPKVRAVRLL
                     RGEAFQDGAPGIYQTIEQEYDSIADIEAALASEVRREMRDKLTEIMPLFKGRIWHINH
                     RLERFL"
     gene            complement(3121..3321)
                     /gene="rpsU"
                     /locus_tag="VGB88_14415"
     CDS             complement(3121..3321)
                     /gene="rpsU"
                     /locus_tag="VGB88_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX9648696.1"
                     /translation="MQVLVRDNNVDQALRALKKKMQREGIFREMKMRRSYEKPSEKRA
                     REKAEAVRRARKLARKRAQRDE"
     gene            complement(3409..3750)
                     /locus_tag="VGB88_14420"
     CDS             complement(3409..3750)
                     /locus_tag="VGB88_14420"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9648697.1"
                     /translation="MYAVIFEFRVKPGQLDAYLGWTERLRERLGKVDGFLSIERFQSL
                     SGEGRYVSISFWRDRAAVTAWRADPLHANAQAEGKAHVFAEFRLRTAKVVHEITLSEA
                     GERAERDEAAL"
     gene            3811..4368
                     /gene="def"
                     /locus_tag="VGB88_14425"
     CDS             3811..4368
                     /gene="def"
                     /locus_tag="VGB88_14425"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9648698.1"
                     /translation="MAILKIARMGHPVLKARAGEVADPTAPEIARLIADMAETLDDAG
                     GIGLAAPQVHVPLRVVIFEVPATRLAEADAEALPRTVLINPVIEPLGDEVAIDWEGCL
                     SLPGLRGAVPRPARVRYSALSPAGEAVAREVGGLHARVVQHECDHLDGILYPMRMRDL
                     STLHYLSEVRVNPDLGDAPRGGDNI"
     gene            4355..5035
                     /locus_tag="VGB88_14430"
     CDS             4355..5035
                     /locus_tag="VGB88_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX9648699.1"
                     /translation="MTTSERERLRRRVLEAALGEVPGHGWTWQAVEAGARDAGLDAAA
                     ARRAFPRGIGEAYDFFLAEADRRMIEALERKDLAALPVRERIATAVRVRLEQAEPHRE
                     AVRRALALQLVPDYALGALPALARTVDAMWRAAGDTATDFNYYTKRALLAAVYGATLL
                     YWLDDRSPGYAETWAFLDRRIDDVMGVEKLRARARALAAELDAPLKRLLARLGRGAAR
                     DWQPPPEA"
     gene            complement(4968..6128)
                     /locus_tag="VGB88_14435"
     CDS             complement(4968..6128)
                     /locus_tag="VGB88_14435"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9648700.1"
                     /translation="MAAPRVIAPGAAIGILGGGQLGRMTALAAARLGYRCHVFGPEAG
                     APASQVAARTTVAAFDDAAALERFAAAVDVVTFEFENVPHEAAEALGRQVSVRPGANA
                     LAIAQDRLEEKRFFASIGAAAAAYRPVRGPGEVEAALAALAAPCVLKTTRLGYDGKGQ
                     VRIDHARDAAAAWARLAAPVGIVERLVDFACEISVVIARGADGATAAYCPVENRHRNH
                     ILDVTVAPADVPAPVARAAGEIALRAAEALELVGLLAVEMFVTAAGEVLVNEIAPRPH
                     NSGHWTIDACATSQFEQLVRAICGLPLGSPERHSDAVMTNLLGADAGRWAEILAEPGA
                     RLHLYGKAEARPGRKMGHVTRLKPRGDGGPATPRAAAASRGRRRGRGGRAGA"
     gene            complement(6297..8222)
                     /gene="ftsH"
                     /locus_tag="VGB88_14440"
     CDS             complement(6297..8222)
                     /gene="ftsH"
                     /locus_tag="VGB88_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9648701.1"
                     /translation="MNNFGRNIALWIIIALLLLTLFNLFQGQSARTSATTLAYSEFLD
                     RVTDGQVRDVTIEGNYLNGHFSGTGQAFTTYAPQDTGLVERLRDNGVLITAKPDKEGV
                     TPLYGILLNWLPMILIVAVWIFFMRQMQGGSGKAMGFGKSRAKLLTEKQGRVTFEDVA
                     GIDEAKEELQEIVEFLRDPHKFQRLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQGRKNAPCIIFIDEIDAVGRHRGAGLGGGNDER
                     EQTLNQLLVEMDGFDSTEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVLGREK
                     ILKVHMRKVPLAPDVDARVIARGTPGFSGADLANLVNEAALLAARKSKRVVTMAEFEE
                     AKDKVMMGAERRSMVMTEEERKLTAYHEAGHALVGLYVAGNDPLHKVTIIPRGRALGL
                     TMNLPEKDRYGFTVKELKGKLAMMFGGRVAEALVYGRDSITTGAGNDIQQATQMARKM
                     VTEWGMSDKLGPLRYEDNQEEVFLGHSVARIQSVSDATARLIDEEIRRLIDDAETQAR
                     AVLEAHRDDLDTVAKTLLEYETLSGAEVEAVLRGESIVRTSDAVPPRDGGRRSSVPVS
                     GKPQAAPDIAPRPQPET"
     gene            complement(8308..9672)
                     /gene="tilS"
                     /locus_tag="VGB88_14445"
     CDS             complement(8308..9672)
                     /gene="tilS"
                     /locus_tag="VGB88_14445"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX9648702.1"
                     /translation="MAGAGVRAAAAEARPVEDAEFARLMAALGPFEPSPAIAVAVSGG
                     VDSLCLALLLERWSRARDGLLLALIVDHGLRPESAAEAARARARLAARGIEARILSWT
                     GPKPGSGIQAAARAARYRLLAAACARVGIVHLALAHHRDDQCETHLLRAAAGSGPYGL
                     AAMAAVVEGRGLRLIRPLLAVPGRRLRATGAAAGLAWDEDPSNRDPRFARARLRAGGG
                     AADADGLAVRIAELGLARADADRRIAALLVRALRLDPAGSALVDLGALAAAPPETALA
                     ALARILCTVSGSLHPPRTARLGRLMATLDSAGLPGRRTLGGCLLVPAAGGRMLVCREP
                     AAIVPAAEVRPGETLLWDGRFTVRLGNARSRRGPLSVRAVDATAWRRLARAAERSASR
                     PAWWPPAVVRAALPVLCDLDGPLALPHLIGCQVAEEAKKIPLAAWFTPRLPATSAAFA
                     AVAT"
     gene            complement(9674..10702)
                     /gene="ybgF"
                     /locus_tag="VGB88_14450"
     CDS             complement(9674..10702)
                     /gene="ybgF"
                     /locus_tag="VGB88_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX9648703.1"
                     /translation="MMVCIRTIAATVLALGVLAALPARPHAQEMSLAALIDRLDRIER
                     DLGGVQRQLAREGVPPAAAAGAAGDPQAAARVEVRMTELEEQFRTLTGAVEEAQHGLR
                     QLVQQYDSLSRDVDARLAAIEQRLAAGAPPPVAGAVPPAGAAPAGLTTPGSEGTTTVV
                     DAGGTTVVGRDANADRYETMGVLGTVPTDEGGAADAAAGELAAVSPAAAAGAPASTPE
                     GQYEDAYRLLAQADYDGAEAALQAFIADHPDHPLVGNAYYWLGETYYVRNDYQEAAVA
                     FARGYKGAPDGSKAADNLLKLGMSFTSMDKTKEACATFDKLLHDHAGAPAQVLSRVEG
                     ERSRAGCS"
     gene            complement(10822..11352)
                     /gene="pal"
                     /locus_tag="VGB88_14455"
     CDS             complement(10822..11352)
                     /gene="pal"
                     /locus_tag="VGB88_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX9648704.1"
                     /translation="MRTKLIALAGVLLLVAACETTPEETGEAGAAGVGAAPQAAPTTT
                     APPEQVEAMAAPRELTPQEQLVQEVGDRVFFDFDRYSLRPDGQATLRRQAEFLLAHPN
                     LSITLSGHCDERGTREYNLALGERRANAAKDYLNSLGVEEDRITTISYGKERPIVLGH
                     NESAWAQNRVGVSSIN"
     gene            complement(11606..12937)
                     /gene="tolB"
                     /locus_tag="VGB88_14460"
     CDS             complement(11606..12937)
                     /gene="tolB"
                     /locus_tag="VGB88_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX9648705.1"
                     /translation="MARVALAAIVGLLAARPASAQLEIDITRGQIEPLPIAVSSFEGP
                     LGVEQQLGADIASVVSKNLESSGLFRPLDPRSFLQRPADMQVIPRFADWRQINAQALV
                     TGKTSIQADGFVTTEFRLWDVFSEQYLTGFEVRAPRDKWRRVGHLVADAIYERITGED
                     GYFDTRIVYVAETGPEDARIKRLAIMDQDGANHRFLTDGAYLVLTPRFSPTLQEITYL
                     SFIGGRPRVYIRNIDTAQEEILGDFPGRMTIAPRFSPDGNKVVMSVSLQGNTELYELD
                     LRTRVQIQLTQHPAIDTAPSYAPDGDQITFESDRSGSQQIYVMNANGSDQHRISFGDG
                     RYATPVWSPRGDWIAFTKSYQGKFYIGVMRPDGSGERLLSTGFLVEGPTWAPNGRVLM
                     FFRQVPGNPSGQGSRVRLVSIDITGRNEREIVTTTDASDPAWSPLVPKGSG"
BASE COUNT         1803 a         4590 c         4695 g         1925 t
ORIGIN      
        1 cctggtcacc ttcctcgcct ccgaccgcgc ctcctacgtc tcgggcgtgg tcatgaccat
       61 cgacggcggc gtcgccgcgc gcgccaggta gcccacagct cagccggcgg gcgccatgct
      121 aagcgtgagg ccgccgtcga ccaccagctc gatgccggtg atgtagcgcg cctcgtccga
      181 ggccaggaac agcgccgcgc gggcggtgtc ccaggcatcg cccatgaacc ccatcgggca
      241 ctgggcgttg cgcttggcga tcatcgcgtc gacgtcgcgg gcgtggagca agtcgtgctc
      301 gagtagcagc agccggcatc ccgtcaggag agttcgacca gcacccggca gtggtcgctc
      361 gggccccatt cgtcgagcga attcagcgct cgcacgcgaa ggcggtggtg cagctcggcc
      421 gacgcgaaga cgaagtcgag ctggcgggtg gccgtcgctg ggtcggcctg gcgaacccga
      481 aacgtcggga cgttcttgct ctcgcgcggt agctctaatg gccaaggctc ggcctggatc
      541 ccgtggggat gctgcggacc gacgaagggg agcccgatgg aggccatccg cgagaagatc
      601 gagaggtacc ggtcgcgcca gtacgggctt ccgtcctctc cgtggcccat caggatgttc
      661 aagtctccgg ctgcgatgat gcgatggccg cgctggctgc cgatgaagac cgaaaggtcg
      721 gataccagcc gatgtgcgga cgcatcggca tagatccatt caccctcgcg tggtcgctcc
      781 cacgctgcat agagcgacac caaggtgagg cgttccccgt tcgccagctc cacgtcggct
      841 gcggcgatcg agccagggcg actgactgcc atttcacgat cattcgcctc cagcaacgag
      901 cgggcggtga ttggtgtgaa ccgaattcga ttgccgaact tcgcaacggc agcgcagaac
      961 aatcgcctgc cacccaccgt actccaggac tcgcttccgg aaggcacggt ttccactgcc
     1021 atgtcgggcg gtggtggaac cgcctcctgt aggagtgcga tgtcgagggc gccgtcccgg
     1081 gcaagttccc tccatgcatc aacggtacgt cgaacattcc aggaaacgag cctcagcacg
     1141 gcagccagga tacgtctgcc ggtactggtc atcaaggcgc gactgcgcac gacaatggca
     1201 ggggcccctc gtgactcacg aggtgggcga ccgcgggatc gcgaagcgag tcggcaagat
     1261 cggcctgggc gccgtcgagc tctcagcggc gacgaaccag gcggcagaac tggctgtagt
     1321 ggacgtcgtc gaggtcgtgt gcaagcccga gcggctggac gacgtgggcg ccgggtgtga
     1381 cgccggggcg gccgccgcgg gagaggtgcc agccgtaatt cgcggcacgg cgcggcggcc
     1441 aggcgcctgc agatgaccca gtctttgtcg gtggtgacga gcccgttccg tccggcgagc
     1501 tcggtctcga gctgcgcgtg ctgccggagg aaggcgctcc tggatgccgt cgtaggccgc
     1561 ggccatgtcg gcgatcagcg tctcgccgtc ttcgtccggc tcctcgagcg catataacat
     1621 ggtcgagatc aaccacagag gacacggagc acacagagac aattcgcctc cgtggcctcc
     1681 gtgctttctg tcgctcccta ttccgcctct ccgcgcctcc gcgtgcccca agcctcctca
     1741 ccggccggca tcctcgccac atgcaactgc attccccgcg cccgagtgga ggcagttgga
     1801 gggtgcttcg gcgcgatagc ggtccggatg cgtcgactgt tcagccctgc gccggcgaca
     1861 tggtgatcgt gagcccgccg tcgaccacca gctcgatgcc ggtgatgtag cgcgcctcgt
     1921 cggaggccag gaacagcgcc gcgcgcgcgg tgtcccaggc gtcgcccatg aaacccatcg
     1981 ggcactgggc gttgcgcctg gcgatcatcg ccgccacgtc gtcataggcg gcggcgaccg
     2041 cgccgcggat cagcggcgta tccatcagcc cgggcagggt cgcgttgcag cgcaccttgt
     2101 ggcgggcgta gtggctggcg atgttgcggg tgagcgcgtt caccgcgccc ttggacgcgg
     2161 cataggaggc gcacgggtag ccgagcgaga agaacgaggc gatcgacgac aggttcacga
     2221 tcgcgccgcc gccttgcgcg accatgtgcg gcagcacgtg gcggcaggtc aggtacatgc
     2281 ttttcacgtt gacgtcgatc agccggtccc aggcgttctc gccgatgtcc tcgggcgtgc
     2341 cgggatcgac gatgccgaca ttgttgtgca gcacgtcgat ccgcccgaag gtgtcgatgc
     2401 acgcctcgac catgtccgcg accgcgccgg ggctcgagac gtcgcagacg tggctggtgc
     2461 atggacagcc ttcgccctcg acgatggccg cggtctcggc gagcgcgtcc ctgttcacgt
     2521 caaccgcaaa caggcgcgcg ccctcgcgcg cgaacagcac cgcggtggcc ttgccgttgc
     2581 cccagcccgg gccgctcgag cccgcgccgg tgacgatcgc gaccttgtcc tgaagcctcc
     2641 ccgccatgcc cgcctccctc gagttcaagg ccgcgggcat ggtagccggc gcccgcgcct
     2701 acaggaagcg ctcgaggcgg tggttgatgt gccagatgcg gcccttgaac aagggcatga
     2761 tctcggtgag cttgtcgcgc atctcgcggc gcacctcgga ggcgagcgcg gcctcgatgt
     2821 cggcgatcga gtcgtactcc tgctcgatgg tctggtagat gccgggggcg ccgtcctgga
     2881 acgcttcgcc gcgcagcagg cgcaccgccc gcaccttggg catggacgtg agcctgggca
     2941 tgatctcggt gtccatgaag cggtcgaagc gcgcgcgcat ctccggcgcg acctcgccct
     3001 cgaaataggc gcagcggatg tgcatggcgt ctcctccctt cgccggcgcg cgctcggtac
     3061 aacatcaaac cgccacccgg gggcggcggc tcggcctggg tccggggccg ggggagcggt
     3121 ctactcgtcg cgctgggcgc gcttgcgggc gagcttgcgc gcccggcgga ccgcctccgc
     3181 cttctctcgc gcgcgcttct cggacggctt ctcgtaggaa cggcgcatct tcatctcgcg
     3241 gaagatgccc tcgcgctgca tcttcttctt cagggcacgc agcgcctggt cgacgttgtt
     3301 gtcgcgaacg agtacctgca agatgcgtcc tttctccggt cacggacggg attcggccga
     3361 cgggccgcgg cttctagcac atggcggggg gattgtgaag gaccgggctt acagcgccgc
     3421 ctcgtcgcgc tcggcgcgct ccccggcctc ggacagggtg atctcgtgca ccaccttggc
     3481 ggtgcgcagc cggaactcgg cgaaaacatg cgccttgccc tcggcctggg cattggcgtg
     3541 cagggggtcg gcgcgccacg ccgtgaccgc cgcccggtcg cgccagaacg agatcgagac
     3601 gtagcggcct tcgcccgaca ggctctggaa gcgctcgatc gacaggaagc cgtcgacctt
     3661 gcccagccgc tcgcgcagcc gctcggtcca gccgagatag gcgtcgagct gccctggttt
     3721 cacccggaac tcgaagatca ccgcatacat gtcttgccct cgcctgtggc gcgctttacc
     3781 gcccggaagc cgcggcccat attagcgcct atggcgatcc tgaaaatcgc ccgcatgggc
     3841 catccggtgc tgaaggcgcg cgccggcgag gtcgccgacc cgaccgcgcc cgagatcgcg
     3901 cgcctgatcg ccgacatggc cgagacgctc gacgatgccg gcggcatcgg cctcgccgcg
     3961 ccgcaggtgc acgtgccgct cagggtggtg atcttcgagg tgccggcgac gcggctggcg
     4021 gaggcggacg ccgaggcatt gccgcgcacg gtgctgatca accccgtgat cgagccgctc
     4081 ggcgacgagg tcgcgatcga ctgggagggc tgcctgtcgc tgcccggcct gcgcggtgcg
     4141 gtgccgcggc cggcgcgcgt ccgctattcg gcgctgtcgc cggcgggcga ggcggtcgcg
     4201 cgcgaggtcg gcggcctgca cgcgcgcgtg gtccagcacg agtgcgatca tctggacggc
     4261 atcctttatc cgatgcgcat gcgcgacctg tcgacgctcc attacctctc ggaggtccgc
     4321 gtcaacccgg acctcggcga cgcgccccgc ggcggtgaca acatctgagc gcgagcgcct
     4381 gcgccggcgc gtgcttgagg cggcgctcgg tgaggtcccc ggccacggct ggacctggca
     4441 ggcggtcgag gcgggcgcgc gcgacgcggg cctcgatgcc gccgccgccc ggcgcgcgtt
     4501 cccgcgcggt atcggcgagg cgtacgactt ctttctcgcc gaggccgacc ggcgcatgat
     4561 cgaggcgctg gagcgtaagg acctcgccgc gctgccggtg cgcgagcgca tcgcgaccgc
     4621 ggtgcgcgtg cgcctcgagc aggccgagcc gcaccgcgag gcggtccgcc gggcgctcgc
     4681 gctgcagctc gtgccggact acgcgctcgg cgccttgccc gcgctcgcgc gcacggtcga
     4741 tgcgatgtgg cgggcggcgg gcgataccgc gaccgatttc aactactaca ccaagcgtgc
     4801 gctgctcgcc gccgtctacg gggcgacctt gctgtactgg ctcgacgacc gctcgccggg
     4861 atacgccgag acctgggcgt tcctcgaccg gcgcatcgac gacgtgatgg gcgtcgagaa
     4921 gctgcgcgcg cgcgcacgcg cgctggccgc ggagctcgat gcgccgctca agcgcctgct
     4981 cgcccgcctc ggccgcggcg ccgcccgcga ctggcagccg ccgcccgagg cgtagccggc
     5041 ccgccgtcgc cgcggggctt gaggcgggtg acgtggccca tcttgcggcc ggggcgcgcc
     5101 tcggccttgc cgtagaggtg caggcgcgcg ccgggctcgg ccaggatctc cgcccagcgg
     5161 ccggcgtcgg cgcccaacag gttggtcatc accgcgtccg aatggcgctc gggcgagccc
     5221 aagggaaggc cgcagatcgc gcgcaccagc tgctcgaact ggctggtcgc gcaggcgtcg
     5281 atggtccagt ggcccgaatt gtgcggcctg ggcgcgatct cgttgaccag cacctcgccg
     5341 gccgccgtga cgaacatctc gaccgcgagc aggccgacca gctccagcgc ctcggccgcg
     5401 cggagcgcga tctcgcccgc cgcccgcgcc accggcgcgg gcacgtcagc cggggcgacg
     5461 gtgacgtcga ggatgtggtt gcggtggcgg ttctcgaccg ggcagtaggc ggcggtggca
     5521 ccgtccgcgc cgcgggcaat gaccaccgag atctcgcacg cgaagtcgac caggcgctcg
     5581 acgatgccga ccggcgccgc gagccgcgcc cacgccgccg ccgcatcgcg cgcgtggtcg
     5641 atgcgcacct ggcccttgcc gtcatagccg aggcgggtgg tcttgagcac gcagggggca
     5701 gcgagcgcgg cgagcgcggc ctcgacctcg ccgggcccgc gcaccggccg gtaggcggcc
     5761 gccgcggcgc cgatcgaggc gaagaatcgc ttctcctcga gccggtcctg ggcgatcgca
     5821 agcgcgttgg cgccggggcg caccgacacc tgccggccga gcgcctcggc cgcctcatgg
     5881 ggcacgttct cgaactcgaa ggtgaccacg tcgaccgcgg ccgcgaaacg ctccagcgcc
     5941 gcagcgtcgt caaaggccgc gaccgtggtc cgggccgcga cctggctcgc cggggcgccg
     6001 gcttcgggac cgaacacgtg gcagcgatag ccgagccggg ccgcggcgag cgcggtcatg
     6061 cgcccgagct ggccgccgcc gaggatgccg atggccgcgc ccggcgcgat cacccgaggg
     6121 gcggccatcg ccgtgcgcgc gctcagagcc cgaaaatctt ttgccgcacc cttcgagacg
     6181 cgcccgtcgg gcgctcctca gggtgaggta aatgattgat tctcaaaccc ccctcgtcct
     6241 gaggagcgag cggagcgagc gtctcgaagg gcgacagccg attcattggc agcctctcag
     6301 gtctcgggct ggggcctggg cgcgatgtcg ggcgcggcct ggggcttgcc gctgaccggc
     6361 accgacgacc gccgcccgcc gtcgcgcggc ggcacggcgt cggaggtgcg cacgatcgac
     6421 tcgccgcgca gcaccgcctc gacctcggcg cccgacaagg tctcgtactc gagcagcgtc
     6481 ttggccacgg tgtcgaggtc gtcgcgatgc gcctcgagca ccgcgcgcgc ctgcgtctcg
     6541 gcgtcgtcga tcagcctgcg gatctcctcg tcgatcagcc tggcggtggc gtctgagacg
     6601 ctctggatgc gcgccaccga gtggccgagg aacacctctt cctggttgtc ctcgtagcgc
     6661 agcgggccga gcttgtcgct catgccccac tcggtgacca tcttgcgcgc catctgggtc
     6721 gcctgctgga tgtcgttgcc ggcgccggtg gtgatgctgt cgcggccgta gaccagcgcc
     6781 tcggcgaccc ggccgccgaa catcatcgcc agcttgccct tgagctcctt gacggtgaag
     6841 ccgtagcggt ccttctcggg caggttcatg gtcaggccga gggcgcgccc gcgcgggatg
     6901 atggtgacct tgtgcagcgg gtcgttgccg gccacgtaca ggccgaccag cgcgtggccc
     6961 gcctcgtgat aggcggtcag cttgcgctcc tcctcggtca tcaccatcga gcgccgctcg
     7021 gcgcccatca tcaccttgtc cttggcctcc tcgaactcgg ccatggtgac cacgcgcttg
     7081 gacttgcgcg ccgccagcag cgccgcctcg ttgaccaggt tggcgagatc ggcgcccgag
     7141 aagccgggcg tgccgcgcgc gatcacccgc gcatccacgt ccggcgccag cggcaccttg
     7201 cgcatgtgca ccttgaggat cttctcgcgc cccagcacgt cggggttggg caccaccacc
     7261 tggcggtcga agcggcccgg gcgcagcagc gccgggtcga gcacgtcggg ccggttggtg
     7321 gcggcgatca ggatcacgcc ctcggtcgag tcgaagccgt ccatctcgac caggagctgg
     7381 ttcagggtct gctcgcgctc gtcgttgccg ccgcccaggc cggcgccgcg gtggcggccg
     7441 accgcgtcga tctcgtcgat gaagatgatg cacggcgcgt tcttgcgccc ctgctcgaac
     7501 atgtcgcgca cccggctcgc gccgacgccg acgaacatct cgacgaagtc cgagcccgag
     7561 atggtgaaga acggcacgtt ggcctcgccg gcgatggcgc gcgcgagcag ggtcttgccg
     7621 gtgccgggcg ggccgaccag cagcacgccc ttggggatgc gcccgccgag gcgctggaac
     7681 ttgtgcgggt cgcgcaggaa ctcgacgatc tcctgcagct cctccttggc ctcgtcgatg
     7741 ccggcgacgt cctcgaacgt cacccggccc tgcttctcgg tcaacagctt cgcccgcgac
     7801 ttgccgaagc ccatcgcctt gccgctgccg ccctgcatct ggcgcatgaa gaagatccac
     7861 accgccacga tcaggatcat cggcagccag ttgagcagga tgccgtaaag cggcgtgacg
     7921 ccttccttgt cgggcttggc ggtgatcagc acgccgttgt cgcgcaggcg ctcgaccaag
     7981 ccggtgtcct ggggcgcgta ggtggtgaac gcctggccgg tgcccgagaa atggccgttg
     8041 agatagttgc cttcgatggt gacgtcgcgg acctggccgt cggtcacccg gtcgaggaac
     8101 tcggagtagg cgagcgtggt cgcgctggtc cgtgccgact ggccctgaaa caggttgaac
     8161 agggtcagca gcagcagcgc gatgatgatc cacagcgcga tattgcggcc gaaattattc
     8221 aagctctttc cctcgaagcc ttcccgcccg gggatgcgcg gcctcggtgc ggccatccta
     8281 gcagatcggg cacggttgcg gcgcagttca tgtggcgacc gccgcgaagg ccgccgacgt
     8341 cgccggcagg cgcggggtaa accacgccgc aagcggaatt ttcttcgcct cctcggcgac
     8401 ttgacaccca attaggtggg gcagggcgag gggaccgtca aggtcgcaaa ggaccggcag
     8461 cgccgccctc accaccgccg gcggccacca agcggggcgg gatgcggacc gttccgctgc
     8521 gcgcgcgagc cggcgccagg cggttgcatc gaccgcgcgc accgacagcg gaccgcgccg
     8581 ggaccgggcg ttgccgaggc ggaccgtgaa gcgaccgtcc cacaagaggg tctcgccggg
     8641 ccgcacctcg gccgccggca cgatcgccgc tggctcccgg cacaccagca tccgcccgcc
     8701 cgcggcaggc accagcaggc agccgccgag ggtgcgccgg ccggggaggc cggcggagtc
     8761 cagcgttgcc atcaggcgcc cgaggcgggc ggtgcgcggc ggatgcaggg agccggagac
     8821 cgtgcacagg atgcgcgcga gtgcggccag cgcggtctcc ggcggggccg cggcgagcgc
     8881 gccgaggtcg accagcgcag agccggccgg gtccaggcgc agcgcccgca ccagtagcgc
     8941 ggcgatgcgc cggtcggcgt cggcgcgggc gagcccaagt tcggcgatgc gcacggcgag
     9001 gccatcggca tcggctgcgc cgccgccggc gcgcagccgg gcgcgcgcga acctggggtc
     9061 gcggttcgac gggtcctcgt cccaggcgag cccggccgcg gcgccggtcg cgcgcagccg
     9121 ccggccgggg acggcgagca gcgggcggat caggcgcagg ccgcggccct cgaccaccgc
     9181 cgccatggcc gcgagaccgt agggtccgct gccggcggcg gcgcgcaaca ggtgggtctc
     9241 gcactggtcg tcgcggtgat gggcgagcgc caggtggacg atcccaacgc gcgcgcacgc
     9301 cgccgctagc agccggtagc gggccgcgcg cgccgccgcc tggatgccgc tcccgggctt
     9361 gggaccggtc cacgacagga tgcgcgcctc gatgccgcgc gcggcgagcc gcgcgcgcgc
     9421 gcgcgccgcc tccgccgccg actccgggcg caggccgtgg tcgacgatca gcgccagcag
     9481 caggccgtcg cgggcgcgac tccagcgctc gagcagcagc gcgaggcaca ggctgtcgac
     9541 gccgcccgag accgcgaccg cgatcgcggg agaaggctcg aacggcccca gcgccgccat
     9601 caggcgggcg aactcggcgt cctcgaccgg gcgggcctcg gctgcggccg cgcgcacgcc
     9661 ggcgccggcc atgtcagctg cagccggcgc ggctccgctc gccctcgacc cggctcagca
     9721 cctgcgccgg cgcgccggca tggtcgtgca aaagcttgtc gaacgtcgcg caggcttcct
     9781 tggtcttgtc catcgaggtg aacgacatgc ccagcttgag caggttgtcg gcggccttgg
     9841 agccgtccgg cgcgcccttg tagccgcgcg cgaaggcgac cgcggcctcc tggtagtcgt
     9901 tgcgcacgta ataggtctcg cccagccagt aataggcgtt gccgaccaag ggatggtcgg
     9961 gatggtcggc gatgaaggcc tgcaacgccg cctcggcgcc gtcgtagtcg gcctgcgcca
    10021 gcaggcggta ggcgtcctcg tactggccct cgggcgtcga tgcgggggca ccggccgcgg
    10081 cggcagggga aaccgccgca agctcgccgg cggcggcatc ggcggcgccg ccctcgtcgg
    10141 tcgggacggt gccgagcacg cccatggtct cgtagcggtc ggcgttggcg tcgcgcccga
    10201 ccacggtggt gccgccggca tcgaccaccg tcgtcgtgcc ctcggagccc ggcgtggtca
    10261 ggccggccgg tgccgccccg gcaggcggca ccgcgccggc gaccggcggc ggggcgcccg
    10321 ccgccagccg ctgctcgatc gccgccagcc gcgcatccac gtcccgggac agcgagtcgt
    10381 actgctgcac cagctggcgc aggccatgct gggcctcctc gaccgcgccg gtgagcgtgc
    10441 ggaactgctc ttccagctcg gtcatccgga cctccacgcg ggcggcggcc tgcgggtcgc
    10501 cggcagcgcc cgcggcggcg gccggcggca cgccctcgcg ggcgagctgg cgctggacgc
    10561 cgccgaggtc gcgctcgatc cggtcgagcc ggtcgatcaa cgcggcgagg ctcatctcct
    10621 gcgcgtgcgg gcgcgccggc aaggccgcca gcacgcccag cgccagcact gtcgccgcga
    10681 tggttctgat gcagaccatc atcgatgcca tccgtcggta ttcgcgccca gcctgcatgc
    10741 catgtagtca cgacatcgtg gcgatagtag ggcggcgcgc gcaccgccgc gccgcccggc
    10801 accggccgtg tcggcgcggc gctagttgat cgagctgacc cccacccggt tctgcgccca
    10861 cgccgattcg ttgtggccga gcacgatcgg tcgctccttg ccgtaggaga tggtggtgat
    10921 gcggtcctct tcgacgccga gcgagttcag gtagtccttg gccgcattcg cgcgccgctc
    10981 gccgagcgcc aggttgtact cgcgggtgcc gcgctcgtcg caatggccgc tgagggtgat
    11041 cgacaggttg ggatgggcga gcaggaactc ggcctggcgg cgcagcgtcg cctggccgtc
    11101 cgggcgcagg ctgtagcggt cgaaatcgaa gaacactcga tcgccgacct cctgcaccag
    11161 ctgctcctgc ggcgtcagct cgcgcggcgc cgccatggcc tcgacctgct ccggcggcgc
    11221 ggtcgtggtg ggggcggcct gaggcgcggc cccgactccc gcggctccgg cctcgccggt
    11281 ctcctcgggc gtggtctcgc aagccgcgac aagcagcagc acgcccgcaa gggcgatcag
    11341 tttcgtccgc atttacgctg tctcctgatg aataccattt cgcagggcgc caggatcacc
    11401 ggcagcctcg ggccgatccc gtgtcggccc acggcggcca ggacccgtca tcgacaaccc
    11461 cctggtgaaa acgcccgagt agcgggaccc cgctcgcccc cgcgcgcatg tgagaagcga
    11521 ttctattttg agaccggttg cgtatcaagc cctgcccggc ctccgggcag tcccggcacc
    11581 ggttgccgga tcgaatttca cgtgatcagc ccgagccctt cggcaccagc ggcgaccacg
    11641 ccgggtccga ggcgtcggtg gtggtgacga tctcgcgctc gttgcgcccg gtgatgtcga
    11701 tcgacaccag gcgcacccgg ctgccctggc cgctggggtt gccgggcacc tggcggaaga
    11761 acatcagcac ccggccgttg ggcgcccagg tcggcccctc gaccaggaag ccggtcgaca
    11821 gcaggcgctc gcccgagccg tccgggcgca tgacgccgat gtagaacttg ccctggtagg
    11881 acttggtgaa cgcgatccag tcgccgcgcg gcgaccacac cggcgtggcg tagcgcccgt
    11941 cgccgaagct gatccggtgc tggtcgctgc cgttggcgtt catgacgtag atctgctggc
    12001 tgcccgagcg gtcggactcg aaggtgatct ggtcgccgtc cggggcgtag ctcggggcgg
    12061 tgtcgatcgc cggatgctgg gtcagctgaa tctggacccg ggtcctgagg tcgagctcgt
    12121 agagctcggt gttgccctgc agcgacaccg acatcaccac cttgttgccg tcgggcgaga
    12181 acctgggcgc gatcgtcatg cggcccggga agtcgcccag gatctcctcc tgcgcggtat
    12241 cgatgttgcg gatgtagacg cgcggccggc cgccgatgaa ggacaggtag gtgatctcct
    12301 gcaaggtcgg cgagaagcgc ggggtcagca ccaggtaggc gccgtcggtg aggaagcggt
    12361 ggttggcgcc gtcctggtcc atgatcgcca ggcgcttgat gcgcgcatcc tccggcccgg
    12421 tctcggcgac gtagacgatg cgggtgtcga aatagccgtc ctcgccggtg atgcgctcgt
    12481 agatggcgtc ggcgaccagg tggccgacgc gccgccactt gtcgcgcggc gcgcgcacct
    12541 cgaagccggt caggtactgc tccgagaaca cgtcccacag ccggaactcg gtggtcacga
    12601 agccgtcggc ctggatgctg gtcttgccgg tgaccagcgc ctgggcgttg atctggcgcc
    12661 agtcggcgaa gcgcgggatc acctgcatgt cggccgggcg ctggaggaag ctgcgcgggt
    12721 cgagcgggcg gaacaggccc gagctttcga ggttcttgga gaccaccgag gcgatgtcgg
    12781 cgccgagctg ctgctcgacc ccgagcggtc cctcgaagct ggagaccgcg atcggcagcg
    12841 gctcgatctg gccgcgggtg atgtcgatct cgagctgggc ggaggccggc cgggcggcga
    12901 gcaggccgac gatggcggcc agcgcaacgc gtgccaggcg ggcggaacgg tcgctgcgac
    12961 cgggtgtcgg ggcgagtact cggcggcgct cgaggcggcg ccgggcgacg gcc
//
LOCUS       DASWHU010000390        12638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3237730, whole genome shotgun sequence.
ACCESSION   DASWHU010000390 DASWHU010000000
VERSION     DASWHU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237730"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGB88_14465"
     CDS             complement(<1..248)
                     /locus_tag="VGB88_14465"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HEX9648706.1"
                     /translation="MPRKLAHLVAGLGLVVLAATAAVALAQAGSRELKNTGAVGETLN
                     GYLAVTDGGANRDVRGEVETINAARRETYLKAAQDAGRP"
     gene            complement(280..486)
                     /locus_tag="VGB88_14470"
     CDS             complement(280..486)
                     /locus_tag="VGB88_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEX9648707.1"
                     /translation="MHVPSCARPFALILLAGAALSGCQPTVKVEAPDKPIVINLNVKI
                     EQEVKIKIDRELDDAFSENPSLFQ"
     gene            complement(551..3799)
                     /locus_tag="VGB88_14475"
     CDS             complement(551..3799)
                     /locus_tag="VGB88_14475"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEX9648708.1"
                     /translation="MRRVLAATLTSLRRLIFYAVLPGVLAALLAWYVVLPPVAERTVG
                     ERLRALGLDGLSFTIAGIGPDRALVRDIAFPAGHLSADEIALDYGVHALATRRADRLT
                     VTGLRWRTRLVGGRLDLSGLAPLLGGDGDGGAPAVPVAEIVLRDGEVVVDTDAGPVQV
                     PFSGRLRPRAPALLSGELDLAPHARFASATVTVALDADLTAGAVDAVVSVTDGAMSLA
                     GIAAFGVFGEVDLHATAAGVTWSAYVEVAELSSPLGPVAARATVVADSDRAEATIDLG
                     PADRAFALGVAATARLDAAPRLELTAALTVPEPEALARRGASVVVLDEDLPRVTLALD
                     AGAPDIAQLLAATGWAEALRQVTAQGRLEASLPGLSLPGAIEDLAANAALEFRTEAGS
                     VAVTSDAVSLSVQRIRGEWLPLVGAGALAPYLEDGVAVGIAPRAGPMALLTPSADGGM
                     SVRLAGDVHLSLGTALVARAGADAVDLELAGGDGSLALASLKGGELVLETWRERPLGL
                     AIERARIELAGERGRYSGRARIAAAGALAPVAGVVLPETRITVDAGLSLTPGRLVAEL
                     RNRDLAETGGAVLTDAHGLTLAPLRVGAAERQPGPLLDLRFAGGGIERWAARLALVPE
                     ALRMTLPLPGSAAAEATVEIAELSLAADGGRGAPRLALEVARARAEAALPGELMPGLP
                     LSIAAEELSVAATMGPDDPGTVVVEIGRLTQHAPRPLLVPFALSATTRREADGTLAIA
                     AGLREGSGNVVLDFTGRYDPARGQGQGQVILHPVLFVDGGFQPARLSPYFAPRIGPTT
                     GSVSIAGPIAWSDAGLFSDVIVAVENLNTTFDSVAVAGVNAAITLDRLLPPSTPPSQI
                     VALAGIDLGLPLTDGLVWGRLDKRGAPLLERSEWQWAGGRVATSFTSRPETGEDTLVL
                     EIDGIQLDRLLELTAQGSNVEATGTLFGRIPVTFRGGEFVIVEGWLESSFEGGELHYH
                     ADGLAPGLLAGGGGVDLLLSALDNFVYSRLRIDLSGLPDGQTEMRFRIRGANPDLHGG
                     AEIELNMSVTGRLEQIIRQSYEAYRGVPDEVARELARQGRRLGRDDATEGPR"
     gene            3945..4841
                     /locus_tag="VGB88_14480"
     CDS             3945..4841
                     /locus_tag="VGB88_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9648709.1"
                     /translation="MTLQELRYLVALADHRHFGRAAAACHVSQPTLSTQLKKLEAFLG
                     TALFERTNKAVDITPAGAPIVAKARRVLAEAESLVALARAQTGPLVGPLRLGVIPTLG
                     PYLLPWLVPALTRAYPQLRLIVDEDLTDRLIERIEAHKCDAALVALPVGRGDLETRAL
                     FDEPFFYVGQRGRTRTKGKAVDPATLGGADLLLLTDGHCLRDQALAVCGLTQAPDGGD
                     FRATSLETIRQMVAAGMGCSLFPALALDERAGARLDVRRLAPAASRRIGLVWRSSYPK
                     AADLELLAGIVRAHLPRSVRAA"
     gene            4973..6442
                     /locus_tag="VGB88_14485"
     CDS             4973..6442
                     /locus_tag="VGB88_14485"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9648710.1"
                     /translation="MPTTKRPTLTTAAGIPVGDNQNALTAGPRGPLLVQDWQLFEKHA
                     HFNRERIPERVVHAKGSAAHGTLNITGDISRFTKAQALQPGAESPCFLRFSTVAGERG
                     AADAERDVRGFALKVYTEDGNWDLVGNNTPVFFVRDPYKFPDFIRTQKRNPRTNLRNA
                     TAMWDFWSLSPESLHQVTILMSDRGLPSSYRHVNGYGSHTYSLINQAGERSWVKFHFK
                     TAQGIECLTDEEAAQIVGGDRESHQRDLYRSIEAGEFPKWRFCVQVMPEHDADQTPYN
                     PFDLTKVWPHGDYPLIKVGVLELNRNPDNYFAEVEQAAFEPSNVVPGIGFSPDKMLQF
                     RIFAYADAHRYRLGSANYAALPINRPRCPVHSYQRDGAMRFDGNGGGTVNYEPNSFAG
                     PAEDARFKEPPLAISGDADRYDHRAGNDDYTQAGNLFRLMTAKQRELLFANIARTMQG
                     VPEDIVRRQIGHFAKADAAYGVGVAKRLGIDVGRDAAAR"
     gene            6589..7677
                     /gene="dctP"
                     /locus_tag="VGB88_14490"
     CDS             6589..7677
                     /gene="dctP"
                     /locus_tag="VGB88_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9648711.1"
                     /translation="MTILRIGGARRSGVLCAAALAIGLALAGAPAAGETMAIAHLYPE
                     DLTNNEVAPALQHFKDLVEAATGGEVEVEIFGAGALGQEVETGRQAQKGVTVQSVLIS
                     SGAMSSFYKRYQIVATPFLFESYAHAWAFLDSPWFADFMRGTIEESGLRYLGTFDDGG
                     GFVAMTNNKRLIKTVNDFAGLNIRVEENPAHIATMRALGASATPLPWGEVIPALATGL
                     ADGQFNAPVVSVGFKLWEVNDYTTWTGHVYNTVTWLVSEAWLRGLPEPAQRAIVGAAR
                     ESIAIAHGTAAHLAVLGWTTSCSKFKACHVLADDEREAMRARTRPAFKTWITGDFGLP
                     AGEVEAFWAEVERVRAQVEADSIATYLK"
     gene            7691..8215
                     /locus_tag="VGB88_14495"
     CDS             7691..8215
                     /locus_tag="VGB88_14495"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9648712.1"
                     /translation="MAAARRATTLLHKVRRFSDALDRAVIGLCVALVAAMLLVSLAGI
                     AWQAATGHALSWTYSIARMLIPWIAMLSVTCAFKQGEHIAMTMLLARAPRPVIVAAEA
                     LGVLSIAAFAAALAWTGAGFLAESGQLVMISDSIQVSDRWVNAAVPLSGLVLLVHSVD
                     GTALLGAHDAGARE"
     gene            8212..9498
                     /locus_tag="VGB88_14500"
     CDS             8212..9498
                     /locus_tag="VGB88_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9648713.1"
                     /translation="MTAAAGLFLVLLLVGVPIGVVMVAAGIAGAVSVGGLGFIDILAD
                     RFFSGVSGFVLIAIPYFIFTAEIMNRSGLTDRMVAFANSLFGRMPGALSHVNVTVSLF
                     FAGITGAAVTDTVAIGKTLIPAMTREGYGAAYSAAVTACSSIIGPIIPPSIIMIVYAA
                     TLRDISVLQLFAAGVVPGLLMAGALLVASGWVSWRRGYAGHQPVVLRRILVTGLATLP
                     AFGIPILIIGGILSGITTLTEAGALGALYTFALGAAVYRSIDARALWQALVSTVRFSG
                     VVFLLLGASTVLGWTVTRSGIARDAATAIAAVSDDPLVQILVVNALLIALGMVVDVLP
                     AVVVAAPVLAPAMVALGFDPLHFGMVMLLALNLGNITPPVGMTLMTASRIADVPYESA
                     IRESLPFLASHLVVIALASASPALVLWFPRLIGAID"
     gene            complement(9502..11100)
                     /locus_tag="VGB88_14505"
     CDS             complement(9502..11100)
                     /locus_tag="VGB88_14505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9648714.1"
                     /translation="MTDALAQAPAAVVVDRLAHAPPERVGARAWGAFVVMVLGMYVAL
                     VNVQIVGSSFKEIQAGLGAGADQISWVLSAALIAEVIMIPLSGWLARLLSSRWLFTFC
                     TGGFALATLGAANAPTIEAMIAFRALQGFSGGALMPLTLSTTYLCFPQRLHTILVTLV
                     ALFGTSSVAVGPTLGGWLTEILGWRWIFWVPLPFAVIFMVGGNLLIRFDRPDRDLARH
                     IDFLGVALAGIFLGSLLYVLEEGQRSDWFDSDLIVILAVVAGLAAYLFIWREFSIRYP
                     VVDLRVFANRDFAIACFYIAIFGALLYIPIFILPLFLAEVRAIDTWAIGTIVAVLGVS
                     MVISGGIAGVMLRYMRRRTVAALGFAGLALGTWLQGFLTAEYGFSDLVLPQAIRGLAS
                     QMCWLSLVTLAVGNLPPEQVKNGAALFNTVMRLGAAISIAVANAMIVLRSRVHYAEIA
                     AGVPAGHPGESQVLPSVEALFQALHGASPEAARAGVVVISELAQREALIRAYNDVTQG
                     AALLCAASLLLMPLMRGRAAARRR"
     gene            11266..12477
                     /locus_tag="VGB88_14510"
     CDS             11266..12477
                     /locus_tag="VGB88_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648715.1"
                     /translation="MAGGLAIPAAPAAGDGWQAEPAVEELSGSVALEGRLFPSPPQFD
                     GQERHSASLVIEPEYYVAWDDDTSLTVSPFLRLDSADTERTHFDLRELYLHMVGDDWE
                     LGAGVGKVFWGRTESVHLVDIVNQTDQIENIDLEDKLGQPMIDLSLIRDWGYLDFFYL
                     PYSRERTFQGRHGRLRNALVVDRSLTRFESGVENWHPDVAVRYSNVFGDWDVGLYQFY
                     GTSREPTLALGLTPGGAPVLVPTYELIYQTGADVQYTTGAWLWKLEALYRNGQRDRIG
                     VEHDYAAFAGGFEYTLFGVADSDADIGLLAEYLRDSRLGKATNAFQNDLFLGARLTLN
                     DADDTSVLAGVVRDLSNGSRLFSVEASRRLGTSLVLSLEARLFSSVDAGDVLSDLRDD
                     DLIQLELGYYF"
BASE COUNT         1910 a         4527 c         4423 g         1778 t
ORIGIN      
        1 ggccggccgg catcctgggc ggccttgaga taggtctcgc gccgggcggc gttgatcgtc
       61 tcgacctcgc ccctgacgtc ccggttggcg ccgccgtcgg tgaccgcgag atagccgttg
      121 agagtctcgc cgaccgcgcc ggtgttcttg agctcgcgcg agccggcctg ggcgagcgcg
      181 accgcggcgg tggcggcgag cacgaccagt ccgagacccg cgaccaggtg ggcaagcttg
      241 cgcggcatgg cggttcccgt tgctggatgt tcggtcatgt cattggaaca ggctcgggtt
      301 ttccgagaac gcgtcgtcca gctcgcggtc gatcttgatc ttgacctcct gctcgatctt
      361 gacgttgagg ttgatcacga tcggcttgtc cggcgcttcc accttgaccg tcggctggca
      421 tccggacagg gcggcgccgg cgagcaggat cagggcaaac ggccgggcgc aactgggcac
      481 atgcattggc gcactctcga aaatgctggt tggctaaccg ggccggtccg tccgcgccgt
      541 cgccgccgcg tcatcggggc ccctcggtgg cgtcgtcgcg gccgagcctc cggccttgtc
      601 gcgcgagctc gcgggcgacc tcgtcgggga ccccacggta ggcctcatag ctctgtcgga
      661 tgatttgttc aagcctgccg gtaaccgaca tgttgagctc tatctcggcg ccgccatgga
      721 ggtcggggtt cgcgccccgg atgcgaaagc gcatctcggt ctggccgtcg ggaaggccgc
      781 tgaggtcgat ccgcagccgg ctgtagacga aattgtcgag cgccgagagc agcaggtcaa
      841 ccccgccgcc gccggcgagc agccccggcg ccagcccgtc ggcatggtag tgcagctcgc
      901 cgccctcgaa gctcgattcg agccagccct cgacgatcac gaactcgccg ccgcggaagg
      961 tcacggggat gcgcccgaac agggtgccgg tcgcctcgac gttcgagccc tgggcagtca
     1021 gctcgagcag ccggtcgagc tggatgccgt cgatctcgag caccagcgtg tcctcgccgg
     1081 tctcgggccg gctggtgaac gatgtcgcca cccggccgcc ggcccactgc cattcgctgc
     1141 gctcgagcag cggcgcgccg cgcttgtcga gccggcccca caccagcccg tcggtcagcg
     1201 gcaggcccag gtcgatgccg gcgagcgcca cgatctggct gggcggcgtc gacggcggca
     1261 gcaggcggtc gagggtgatg gcggcgttga ccccggcgac ggcgacgctg tcgaaggtgg
     1321 tgttcaggtt ctcgaccgcg acgatgacgt cgctgaacag gccggcatcc gaccaggcga
     1381 tcgggccggc gattgacacc gatcccgtgg tcggcccgat gcgcggcgcg aagtagggcg
     1441 acaggcgcgc cggctggaag ccgccgtcga cgaacagcac cggatgcagg atgacctggc
     1501 cctggccctg gccgcgcgcc ggatcgtagc gcccggtgaa atcgagtacg acattgccgc
     1561 tgccctcgcg cagtccggcg gcgatggcga gcgtgccgtc ggcctcgcgc cgggtggtgg
     1621 cggagagggc gaacggcacc agcagcgggc ggggcgcgtg ctgggtcagg cggccgatct
     1681 cgaccaccac cgtgcccgga tcgtcggggc ccatggtcgc cgccaccgac agctcctcgg
     1741 cggcgatgct gagcggcagg ccgggcatca gctcgcccgg cagcgcagcc tcggcgcgcg
     1801 cgcgcgcgac ctcgagcgcg agcctgggcg cgccgcggcc gccgtcggcg gcgaggctga
     1861 gctcggcgat ctcgaccgtc gcctcggcgg ccgctgaacc gggcagcggc aaggtcatgc
     1921 gcagcgcctc cgggaccagc gcgagccgcg ccgcccagcg ctcgatcccg ccaccggcga
     1981 agcgcaggtc gagcagcggc ccgggctggc gctcggcggc gccaacgcgc aacggcgcga
     2041 gcgtaagacc gtgcgcgtcc gtgagcaccg cgcccccggt ctcggccagg tcgcgattgc
     2101 gcagctcggc aaccagccgg ccgggggtga gcgacagccc ggcatcgacc gtgatccggg
     2161 tctcgggcag gaccacgccc gcaaccgggg cgagggcgcc cgcagcggcg atgcgggcgc
     2221 ggccgctgta acggccgcgc tcgcctgcca gctcgatgcg cgcgcgctcg atcgcgaggc
     2281 ccagcggccg ctcgcgccag gtctccagca ccagctcgcc acccttgagc gacgccaagg
     2341 cgagcgaccc gtcgccgccg gcaagctcca gatcgaccgc gtcggcaccg gcccgcgcga
     2401 ccagggcggt gccgagcgaa aggtgcacgt cacccgccag gcgcaccgac atgccgccgt
     2461 cggcggaggg cgtcaaaagc gccatcgggc ccgcgcgcgg cgcgatgccg accgcgactc
     2521 cgtcctcgag atagggcgcg agcgcgccgg ctcccaccaa cggcaaccat tcgccccgta
     2581 tccgttgcac cgacagcgaa acggcatcgc tggtcacggc cacgctgccg gcctcggtgc
     2641 ggaactccaa agcggcgttg gccgccaggt cctcgatcgc tccgggcagc gatagccccg
     2701 gcagcgatgc ctccagccgg ccctgggccg tgacctgtcg cagcgcctcg gcccagccgg
     2761 ttgccgccag cagctgcgcg atatcgggcg cgcccgcatc gagggcgagc gtcacccgcg
     2821 gcaggtcctc gtccagcacg accaccgacg cgccgcgccg ggccagcgcc tcgggctccg
     2881 gcacggtgag cgcggcggtg agctcgagcc gcggcgcggc gtcgagccgg gcggtcgccg
     2941 cgaccccgag cgcgaacgcc cggtcggccg ggccgaggtc gatggtcgcc tcggcgcggt
     3001 cggagtcggc gaccacggtc gcgcgcgcgg ccacggggcc gagcggcgat gaaagctcgg
     3061 cgacctcgac ataggctgac caggtgacgc cggccgcagt ggcatgcagg tccacctcgc
     3121 caaagacgcc gaaggcggcg atgccggcaa gcgacatcgc gccgtcggtg accgaaacga
     3181 cggcgtcgac cgcgcccgcg gtgagatcgg catcgagcgc gaccgtcacc gtggccgagg
     3241 cgaagcgcgc atggggcgcg aggtcgagct cgccggacag gagcgccggg gcgcgcgggc
     3301 gcagccggcc cgagaacggc acctgcaccg gccccgcgtc cgtgtcgacc accacctcgc
     3361 cgtcgcgcag cacgatctcg gccaccggca ccgccggcgc gccgccgtcg ccgtcgccgc
     3421 cgagcaacgg cgcaagcccg gacaggtcga gccggccgcc gaccaggcgc gtccgccaac
     3481 gcaagccggt gacggtcagc cggtcggcgc gcctggtggc gagcgcgtgc acgccgtagt
     3541 cgagcgcgat ctcgtcggcg gacaggtggc cggccgggaa ggcgatgtcg cgaaccaatg
     3601 cccggtccgg gccgatgccg gcgatggtga agctgaggcc gtcgaggccg agcgcgcgta
     3661 gccgctcgcc gaccgtgcgc tcggccaccg gcggcagcac cacgtaccag gcgagcaggg
     3721 ccgcgagcac gcccggcagc acggcataga agatcagccg cctgagcgag gtgagggttg
     3781 ccgccagaac cctgcgcacc gagccgaatc tcccctctgc gggtgacgat ggtgccgtcg
     3841 cgcgcgctgt gcgtcaaaag cgcgccggtt gaagcgcgtc cggatagccc aatatagatc
     3901 gaccacgcat caacaatcga tgatgactat cgccggtgcg cgccatgacg ttacaggagc
     3961 tccgctacct ggtcgcgctt gccgaccacc gccatttcgg ccgcgcggcc gcggcctgcc
     4021 acgtcagcca gccgaccctc agcacccagc tcaagaagct cgaggcgttc ctcggcaccg
     4081 ccctgttcga gcgcaccaac aaggcggtcg acatcacccc ggccggagcg ccgatcgtgg
     4141 ccaaggcgcg ccgggtcctg gccgaggccg agtccctcgt cgccttggcg cgcgcgcaga
     4201 ccgggcccct ggtcgggccg ctccgtctcg gcgtcatccc gacccttggg ccgtacctgc
     4261 tgccctggct ggtgccggcg ctgacgcgcg cctatccgca gctcaggctg atcgtcgacg
     4321 aggacctcac cgatcgcctg atcgagcgaa tcgaggccca caagtgcgat gccgcgctgg
     4381 tcgcgctgcc ggtcgggcgg ggcgacctcg agacgcgcgc cctcttcgac gagccgttct
     4441 tctacgtcgg tcagcgaggg cgcacgcgca ccaagggcaa ggcggtcgac ccggccaccc
     4501 tcggaggcgc ggacctgctg ctgctcaccg acgggcattg cctgcgcgac caggcgctcg
     4561 cggtctgcgg cctgacgcaa gcgccggacg gcggcgactt ccgcgccacc agcctcgaga
     4621 ccatccgcca gatggtcgcc gcgggcatgg gctgcagcct gtttccggcg ctggcgctgg
     4681 acgagcgtgc gggcgcgcgc ctcgatgtcc gccggctggc gccggcggcg tcgcgccgga
     4741 tcgggctggt ctggcggtcg agctacccga aggccgctga cctggagctg ctcgccggaa
     4801 tcgtgcgcgc ccacctgcca cgctccgtgc gcgcggcctg aattgcggtc atagataggt
     4861 gttatctatc gtagagatat aaacaatcaa tttgactgcg actcctcagt tcttagaata
     4921 aatccaataa atcgatcgcc gcacgcgcgg caatcagccg aggaggatcg tcatgccgac
     4981 caccaagcga ccgacgctca ccaccgccgc cggcattccg gtgggcgaca accagaacgc
     5041 gctcaccgcc gggccgcgcg gcccgctgct ggtccaggac tggcagctgt tcgagaagca
     5101 cgcgcacttc aaccgcgagc gcatccccga gcgcgtggtc cacgccaagg gctcggccgc
     5161 ccacggcacg ctcaatatca ctggcgacat cagtcgcttc accaaggccc aggccttgca
     5221 gccgggagcc gagagcccgt gcttcctgcg cttctcgacg gtcgccggcg agcgcggcgc
     5281 ggcggacgcc gagcgcgacg tgcgcggctt cgcgctcaag gtctacaccg aggacggcaa
     5341 ctgggacctg gtcggcaaca acacgccggt gttcttcgtg cgcgatccat acaagttccc
     5401 ggacttcatc cgcacccaga agcgcaaccc gcgcaccaac ttgcgcaacg cgaccgcgat
     5461 gtgggacttc tggtcgctgt cgccggagag cctgcaccag gtgaccatcc tgatgtccga
     5521 ccgcggcctg cccagcagct accggcacgt caacggttac ggctcgcaca cctacagctt
     5581 gatcaaccag gcgggcgagc ggtcctgggt caagttccac ttcaagaccg cccaaggcat
     5641 cgagtgcctg accgacgagg aggcggccca aatcgtcggc ggcgaccgcg agagccacca
     5701 gcgcgacctc tatcggtcga tcgaggccgg cgagttccct aagtggcgct tctgcgtgca
     5761 ggtgatgccc gagcacgacg cggaccagac gccctacaac cccttcgacc tgaccaaggt
     5821 gtggccgcac ggggactatc cgctgatcaa ggtcggcgtg ctcgagctca accgcaaccc
     5881 ggacaactac ttcgccgagg tcgagcaagc ggcgttcgag ccctcgaacg tggtaccggg
     5941 gatcggcttc tcgcccgaca agatgctgca gttccgcatc ttcgcctatg ccgacgccca
     6001 ccgctaccgg ctgggctcgg ccaactatgc ggcactgccg atcaaccggc cgcgctgccc
     6061 ggtgcactcg taccagcgcg acggcgccat gcgcttcgac ggcaacggcg ggggcacggt
     6121 caactacgag cccaacagct tcgccggccc ggccgaggac gcacgcttca aggagccgcc
     6181 gctcgctatc tcgggcgacg ccgaccgcta cgaccaccgt gcgggcaacg acgactacac
     6241 ccaggcgggc aacctgttcc gcctgatgac ggcgaagcag cgcgagcttc tgttcgccaa
     6301 catcgcccgc acgatgcaag gcgtgcccga ggacatcgtc cgccgccaga tcggccactt
     6361 cgccaaggcg gatgcggctt acggcgtggg cgtcgccaag cgcctcggca tcgatgtcgg
     6421 ccgcgacgca gcggccaggt aacgacccga cgcgcggacc gagccaagtc gtcgcgcgcc
     6481 tccgcgcgct cggcgcttgc ccgccggcag gaccggcgcc gccctcgcat cggcggcgcg
     6541 gctgtactac cgtgcgcgct ggcccgcgtg ctggataggg agggcgccat gaccatcttg
     6601 cgcatcggcg gtgctcgccg gagcggcgtg ctttgcgcgg cggcgctggc gatcggcctg
     6661 gcgctcgccg gcgcgccggc ggcaggcgaa accatggcca ttgcgcatct gtacccggag
     6721 gacctgacca acaacgaggt cgcgccggcg ctgcagcact tcaaggacct ggtcgaggcg
     6781 gccaccggcg gcgaggtcga ggtcgagatc ttcggcgccg gcgcgctcgg ccaggaggtc
     6841 gagaccggcc gccaggcgca gaagggggtc accgtccagt cggtgctgat ctcgtcgggg
     6901 gcgatgtcgt cgttctacaa gcgctaccag atcgtcgcca ccccgttcct gttcgagtcc
     6961 tacgcgcatg cctgggcgtt cctcgattcg ccgtggttcg ccgacttcat gcgcggcacg
     7021 atcgaggaat ccgggctgcg ctatctcggc accttcgacg acggcggcgg cttcgtcgcc
     7081 atgaccaaca acaagcgcct gatcaagacc gtcaacgact tcgccgggct caacatccgg
     7141 gtcgaggaga acccggcgca catcgccacc atgcgcgcgc tcggcgcctc ggcgacgccc
     7201 ttgccgtggg gcgaggtgat ccccgcgctc gccaccgggc ttgccgacgg ccagttcaac
     7261 gcacccgtgg tctcggtcgg cttcaagctg tgggaggtca acgactacac cacctggacc
     7321 ggccacgtgt acaacaccgt gacctggctg gtcagcgagg cgtggctgcg cggattgccc
     7381 gagccggcgc aacgggcgat cgtcggcgcg gcgcgcgagt cgatcgccat cgcccacggc
     7441 accgccgcgc atctcgcggt gctcggctgg accacctcgt gcagcaagtt caaggcctgc
     7501 cacgtgctcg ccgacgatga gcgcgaggcg atgcgcgcgc gcacgcgccc ggcgttcaag
     7561 acctggatca ccggcgactt cggcctgccg gcgggcgagg tggaggcgtt ctgggccgag
     7621 gtcgagcggg tgcgcgccca ggtcgaggcg gactcgatag ccacctatct gaaatagccg
     7681 ggcgccagca atggccgcgg cccggcgcgc cacgaccctg ctgcacaagg tgcgcaggtt
     7741 cagcgacgca ctcgaccgcg ccgtgatcgg actgtgcgtg gcgctggtcg cggcgatgct
     7801 gctggtctcg ctcgccggca tcgcctggca ggcggcgacc gggcacgcgc tgagctggac
     7861 ctactcgatc gcgcgcatgt tgatcccgtg gatcgccatg ctcagcgtga cctgcgcgtt
     7921 caagcagggc gagcacatcg caatgaccat gctgctcgcg cgcgcgccgc ggccggtgat
     7981 cgtcgcggca gaggcgctcg gcgtgctgtc gatcgccgcc ttcgccgcgg cgctggcgtg
     8041 gaccggcgcc ggcttcctcg ccgagtcggg gcagctggtc atgatctcgg actcgatcca
     8101 ggtctcggac cgctgggtga acgcggcggt gccgctgtcg ggactggtgc tgctggtcca
     8161 ctcggtcgac ggcacggcgc tgctcggcgc gcatgacgcg ggcgcgcgcg aatgaccgcg
     8221 gccgcgggcc tgttcctggt cctgctcctg gtcggggtgc cgatcggggt ggtgatggtc
     8281 gccgccggga ttgccggggc ggtgtcggtg ggcggcctcg gcttcatcga cattctcgcc
     8341 gaccggttct tctcgggcgt ctcggggttc gtgctgatcg cgatacccta tttcatcttc
     8401 accgccgaga tcatgaaccg ctcggggctc accgaccgca tggtcgcctt cgccaacagc
     8461 ctgttcgggc gcatgccggg cgcgctcagc cacgtcaacg tcaccgtcag cctgttcttc
     8521 gccggcatca ccggggccgc ggtcaccgat acggtcgcga tcggcaagac cctgatcccg
     8581 gcgatgacgc gcgagggcta cggcgccgcc tacagcgcgg cggtgaccgc gtgctcgtcg
     8641 atcatcggcc cgatcatccc gccgagcatc atcatgatcg tctacgccgc gacgcttcgc
     8701 gacatctcgg tcctccagct gttcgccgcg ggcgtggtgc cggggctgct gatggccggg
     8761 gcgctgctgg tcgccagtgg ctgggtgtcg tggcggcgcg gctatgcggg ccaccagccg
     8821 gtggtgctga ggcgcatcct ggtcaccggc ctcgccacct tgcccgcgtt cggcatcccg
     8881 atcctgatca tcggcggcat cttaagcggc atcaccaccc tgaccgaggc cggcgcgctc
     8941 ggcgcgctgt acaccttcgc gctcggggcg gcggtctatc gcagcatcga tgcgcgcgct
     9001 ctgtggcagg cgctggtctc gacggtgcgc ttctcggggg tggtgttcct gctgctcggc
     9061 gcttcgaccg tgctcggctg gaccgtcacc cgctcgggca tcgcgcgcga cgcggccacc
     9121 gcgatcgccg cggtcagcga cgatccgctg gtccagattc tggtggtcaa cgcgctcctg
     9181 atcgcgctcg gcatggtggt cgacgtgctg ccggcggtgg tggtggccgc tcccgtgctg
     9241 gccccggcga tggtcgcgct cggcttcgac ccgctccatt tcggcatggt catgctgctc
     9301 gcgctcaacc tcggcaacat caccccgccg gtcggcatga ccctgatgac cgcgtcgcgc
     9361 atcgccgacg tgccttacga gtcggcgatc agggaatcgc tgccgttcct ggccagccac
     9421 ctggtggtga tcgcgctcgc cagcgcgagc ccggcgctgg tgctgtggtt cccgcgcctg
     9481 atcggcgcca tcgattagac ctcagcggcg gcgggcggcg gcgcgcccgc gcatcagcgg
     9541 catcagcagc aggctcgccg cgcacagcag cgccgcgccc tgggtgacgt cgttgtaggc
     9601 gcggatcagc gcctcgcgct gggcgagttc tgagatcacc acgacgccgg cgcgcgcggc
     9661 ctcgggcgag gcgccgtgga gcgcctggaa cagcgcctcg acgctcggca gcacctgcga
     9721 ctcgcccggg tggccggccg gcacgccggc cgcgatctcg gcgtaatgga cccggctgcg
     9781 cagcacgatc atggcgttgg cgaccgcaat cgagatcgca gcacccagcc gcatcacggt
     9841 gttgaacagc gcggcgccgt tcttgacctg ctccggcggc aggttgccga ccgcgagcgt
     9901 gaccagcgac agccagcaca tctggctggc aagcccgcgg atcgcctgcg gcagcaccag
     9961 gtccgagaag ccgtactcgg cggtcaggaa gccttgcagc caggtgccga gcgcgagccc
    10021 ggcgaagccg agcgccgcca ccgtgcgccg gcgcatgtag cgcagcatga cgccggcgat
    10081 gccgcccgag atcaccatcg agacgccgag cacggcgacg atggtgccga tggcccatgt
    10141 gtcgattgcg cgcacctcgg ccaggaacag cggcaggatg aagatcggga tgtagagcag
    10201 cgcgccgaag atggcgatgt agaagcacgc gatcgcgaag tcgcggttgg cgaacacgcg
    10261 caggtccacc accgggtagc ggatcgagaa ctcgcgccag atgaacaggt aggcggcgag
    10321 gcccgcgacc acggccagga tcacgatcag gtccgagtcg aaccagtcgc tgcgctggcc
    10381 ttcctcgagc acgtagagca gcgagccgag gaagattccc gccagggcga cgccgaggaa
    10441 atcgatgtgg cgggcgaggt cgcggtcggg gcggtcgaag cggatcaaga ggttgccccc
    10501 gaccatgaag atgaccgcga acggcagcgg cacccagaag atccagcgcc agcccaggat
    10561 ctcggtcagc cagccgccga gcgtcggccc gaccgcgacc gaggaggtgc cgaacaaagc
    10621 gaccagggtc accaggatgg tgtgcaggcg ctggggaaag cacaggtagg tggtcgacaa
    10681 ggtcagcggc atcagcgccc cgccgctgaa gccctgcagg gcgcgaaagg caatcatcgc
    10741 ctcgatggtc ggcgcgttgg cggcaccgag ggtggcgagc gcgaagccgc cggtgcagaa
    10801 ggtgaacagc cagcgcgacg acaacagccg cgccagccag cccgacagcg ggatcatgat
    10861 cacctcggcg atcagcgccg ccgacagcac ccagctgatc tggtcggcgc cggcgccgag
    10921 cccggcctgg atctccttga acgacgagcc gacgatctgg acgttgacca gcgccacgta
    10981 catgcccagc accatgacca cgaaggcgcc ccaggcgcgg gcgccgacgc gctcgggcgg
    11041 cgcgtgggcc agccggtcga cgaccaccgc ggcgggggcc tgggcgagcg cgtcggtcat
    11101 gatcgggccg cgccggccgc cggtggcggg cgcaaggcgg aagccgtgct atagagtggg
    11161 gtcgccgccg gcggggcggc acagcgaccg ccgacgagga ccacgcttcc gaccttcgac
    11221 atgcgcccag gccgtatcac gtcccgacat atctgcgcca tcgccttggc cggcggcctc
    11281 gcgatcccgg ccgcgccggc cgccggcgac ggctggcagg ccgagccggc ggtcgaggag
    11341 ctgtccggaa gcgtcgccct ggagggccgg ctgttcccga gcccaccaca gttcgacggg
    11401 caggagcggc acagcgcatc gctcgtgatc gagcccgaat actatgtcgc gtgggacgac
    11461 gacaccagcc tgacggtcag cccgttcctg cgcctcgaca gcgccgatac ggagcgcacc
    11521 catttcgacc tgcgcgagct gtacctgcac atggtcggcg acgactggga gcttggcgcc
    11581 ggcgtcggca aggtgttctg gggcaggacc gagtcggtcc atctggtcga catcgtcaac
    11641 cagaccgacc agatcgagaa catcgatctc gaggacaagc tcggccagcc gatgatcgac
    11701 ctcagcctga tccgcgactg gggctatttg gattttttct acctgccata ttcccgagag
    11761 cgcaccttcc aagggcgtca cgggcgattg cgcaatgcgc tcgtcgtcga tcggtccttg
    11821 acccgcttcg agtccggggt cgagaactgg catccggacg tcgcggtccg ctacagcaac
    11881 gtgttcggcg actgggacgt cgggctctac cagttctacg gcacctcgcg cgagcccacc
    11941 ctcgcgctcg gtctcacgcc gggcggcgca ccggtgctgg tgcccaccta cgagctgatc
    12001 taccagaccg gcgccgacgt ccagtacacc accggcgcct ggctatggaa gctcgaggcc
    12061 ctgtatcgca acggccagcg cgaccggatc ggcgtcgagc acgactacgc cgccttcgcc
    12121 ggcggcttcg agtacaccct gttcggggtc gccgacagcg acgccgacat cggcctgctc
    12181 gccgagtatc tgcgcgacag ccgcctcggc aaggcgacca acgcgttcca gaacgacctg
    12241 ttcctgggcg cgcgcctgac gctcaacgac gctgacgaca ccagcgtgct cgccggcgtg
    12301 gtccgggacc tgtcgaacgg cagccgcctg ttcagcgtcg aggcgagccg ccgtctcggc
    12361 accagcctcg tgctgtcgct cgaggcgcgc ctgttcagct ccgtcgacgc cggcgacgtg
    12421 ctcagcgacc tcagggacga cgacctgatc cagctcgagc tcggctacta cttctagccg
    12481 ccgccggctg ttaccggccg ctccccggtg tcgggctcgg gacgccggac cgcgcggccc
    12541 gggttgcggg ccgacgcggt ggccggcgaa gcccaagcga cgcgtctagt cttcggactt
    12601 ctgcctgcgc ttggacgtct tcaaaggcag cttgacgt
//
LOCUS       DASWHU010000391         5835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3243281, whole genome shotgun sequence.
ACCESSION   DASWHU010000391 DASWHU010000000
VERSION     DASWHU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243281"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..2245)
                     /gene="rpoD"
                     /locus_tag="VGB88_14515"
     CDS             complement(206..2245)
                     /gene="rpoD"
                     /locus_tag="VGB88_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX9648716.1"
                     /translation="MSKQAAAAEEGESREEQGDGPLLDTVGAGVKRLIAKGRERGYIT
                     YDELNAALPSEQVSSEQIEDTMAMISEMGINLVESDEAEDGAERRVEPELSTSNTSDD
                     ADLGRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREMMIGALWESPLTLQAISE
                     WLQAIEDGRMLLREVIDLEATYGGNGAAPAGASVPAAGAGATAGGQGDAQGNTQGNVQ
                     GNVPGSPEAAAGEAEAGPAGGEAAADGEADEDDDDAEEASMTLSSLEEALRPQVLEAF
                     QEIARTYARMRRVEVKRLEADAANDSLKPATLKRYDKLKGEVRALAAGVRLNNFRIEE
                     LVDELYGRNRRLMTLEGRLLRLAIECKVPRESFLAHHVGRELEANWIRRVTRLKDKGW
                     GIFARKHGPAAQEIRDQVREIAVASGLDVGAFKRIVSTVQKGERESSQAKKEMIEANL
                     RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRS
                     IADQARTIRIPVHMIETINKLVRTSRQMLHEIGREPTPEELAEKLVMPLEKVRKVLKI
                     AKEPISLETPIGDEEDSHLGDFIEDKQAVIPLDAAIQSNLRETTTRVLASLTAREERV
                     LRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDT"
     gene            complement(2328..4205)
                     /gene="dnaG"
                     /locus_tag="VGB88_14520"
     CDS             complement(2328..4205)
                     /gene="dnaG"
                     /locus_tag="VGB88_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9648717.1"
                     /translation="MALPPQFLDELRTRLPASEVIGRAVRLARRGREHVGLCPFHNEK
                     TPSFTVNDDKAFFHCFGCGAHGDIIGFVMQHDGLPFPEAVERLAGEAGLSVPKPTPEA
                     RARAAREASLLEVLDKAADWFARALARDDGAAARAYLDSRGVDAPAQARFRLGYAPGR
                     GRALASLLAGEGIDEALALDAGLIRRAERAGESFDFFRNRLMFPIADGRGRVIGFGAR
                     VLGDGQPKYLNSPEGPVFRKGRTLYNLAGARVPAREAGTVIVVEGYMDVIALSAAGFD
                     NAVAPLGTALTEDQLAALWRLAPEPVLCLDGDTAGRRAAARAAARALPLLGPGRSLRF
                     AFLPEGDDPDSLVRRQGPAAVAGCLERAWPLVEVIWRQAAAGAADTPERRAGLRKRLD
                     ELAAEIADRDVRDQYRAEFRRRFEAAFGAPRAGRKAERGHHVLRPHSAYHGHSAKIAG
                     PAEGEQRERLMLVTLLNHPALIAEVIEELATLSFAAGDCEVLRAALIAAAEGDAQSEH
                     ERLDGYLRAHGLGEAAARLCRRSGFATAPVTESFARPGAHEAEALRGFRHLAARHRRA
                     LLEAEIADAVAALAQQMTDEALAHLAALQHQLESLDRGHGEASPGRAEAGLASPPPGT
                     T"
     gene            complement(4222..4692)
                     /locus_tag="VGB88_14525"
     CDS             complement(4222..4692)
                     /locus_tag="VGB88_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9648718.1"
                     /translation="MSDLRAQFSADLKAAMKAKDTCRVATLRLILATLKDRDIAVRAD
                     GNEDGIGADQILDMLAKMVRQRRDSIDIYRQGGRADLAEREAEEIAVIEGYMPKPLDE
                     AETARAIAEAIAAVDAHSLKDVGRVMAALKERYAGRMDFARAGALAKDRLAGKA"
     gene            4943..>5835
                     /locus_tag="VGB88_14530"
     CDS             4943..>5835
                     /locus_tag="VGB88_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX9648719.1"
                     /translation="MPETPAIAPSSARRWPTAVLVLADGRVIRGRGLGAEAKAVGEVC
                     FNTSMTGYQEILTDPSYAAQIITFTFPHIGNVGANAEDIESLTPAARGCVLCADITEP
                     SNWRAAQHLDGWLASHGLPAIAGVDTRALTHLIRAAGAPSGVLVHDAEGRFDVDALRA
                     EAAAWPGLEGMDLAREVSCTQTYGWEQTRWRIGTGYGTLANPRRHVVAIDYGAKRNIL
                     RCLADAGCRVTVVPAAASADEVLRHAPDGIFLSNGPGDPAATGAYAVPVIRALLESGI
                     PLFGICLGHQLLAIALGARTR"
BASE COUNT          740 a         2162 c         2010 g          923 t
ORIGIN      
        1 gcctcgctta tcccgactgt ctgaacccta cgcttggaag ggctgggcat tggtagctcc
       61 cactcgacgc ggacgttggg agtgtccgcc ttggtcctgt ttcctctcgg cccgaggccg
      121 acctttgccc gccaaagtcc cgcaagcggc gaaagcgggt gcgcaccgct ctatccggct
      181 caaccggctc ccgattccgg acccctcagg tatccaggaa gctcctgagc ttgcggctgc
      241 ggctcgggtg cttgagcttg cggagcgcct tggcctcgat ctggcggatg cgctcgcggg
      301 tgaccgagaa ctgctggccg acctcttcca gcgtgtggtc ggtgttcatg ccgatgccga
      361 agcgcatgcg cagcacgcgc tcctcgcgcg cggtcaggct cgccagcacg cgcgtggtgg
      421 tctcgcgcag gttcgactgg atcgcggcat cgagcgggat caccgcctgc ttgtcctcga
      481 tgaagtcgcc caagtggctg tcctcctcgt cgccgatcgg ggtctcgagg ctgatcggct
      541 ccttggcgat cttgagcacc ttgcgcacct tctcgagcgg catcaccagc ttctcggcga
      601 gctcctcggg agtcggctcg cggccgatct cgtgcagcat ctggcgcgag gtgcggacca
      661 gcttgttgat cgtctcgatc atgtgcaccg ggatgcggat ggtgcgcgcc tggtcggcga
      721 tcgagcgcgt gatcgcctgg cggatccacc aggtggcgta ggtcgagaac ttgtagccgc
      781 gccggtactc gaacttgtcg accgccttca tcaggccgat gttgccctcc tggatcaggt
      841 ccaggaactg caggccgcgg ttggtgtact tcttggcgat cgagatcacc aggcggaggt
      901 tcgcctcgat catctccttc ttggcctggc tcgattcgcg ctcgcccttc tgcacggtcg
      961 agacgatgcg cttgaacgcg ccgacgtcga ggccgctggc caccgcgatc tcgcggacct
     1021 ggtcgcggat ctcctgcgcc gccgggccgt gcttcctggc gaagatgccc cagcccttgt
     1081 ccttgagccg ggtgacgcgc ctgatccagt tcgcctcgag ctcgcgcccg acatggtggg
     1141 cgaggaagct ctcgcgcggc accttgcact cgatggcgag cctcaagagc cgcccctcca
     1201 aggtcatcag ccggcggttg cggccgtaca gctcgtcgac cagctcctcg atgcggaagt
     1261 tgttgagcct gaccccggcg gcgagcgcgc gcacctcgcc cttgagcttg tcgtagcgct
     1321 tgagcgtcgc cggcttgagc gaatcgttgg cggcgtcggc ctccaggcgc ttgacctcga
     1381 cccggcgcat gcgggcgtag gtcctggcga tctcctggaa cgcctcgagc acctgcggcc
     1441 tgagcgcctc ctcgagcgag gacaaggtca tgctcgcctc ttcggcgtcg tcgtcgtcct
     1501 cgtccgcctc gccgtcggcc gcggcctcgc cgccggcggg gccggcctcg gcctcgcccg
     1561 ccgcagcctc gggcgagccc gggacgttgc cctggacgtt gccctgggtg ttgccctggg
     1621 cgtcgccctg cccgcctgcc gtcgcgccgg cgccggccgc cggcacgctc gcgccggcgg
     1681 gcgccgcgcc gttgccacca taagttgctt ctaagtcgat cacctcgcgc agcagcatcc
     1741 gcccgtcctc gatcgcctgc agccactcgc tgatcgcctg cagggtcagc gggctctccc
     1801 acagcgcgcc gatcatcatc tcgcgaccgg cctcgatgcg cttggcgatg gcgatctcgc
     1861 cctcgcgcga cagcagctcg accgagccca tctcgcgcag gtacatgcgc acggggtcgt
     1921 cggtgcggcc gaggtccgcg tcgtccgagg tgttggacgt cgacagctcc ggctcgacgc
     1981 gccgctcggc cccgtcttcg gcttcgtcgc tctcgaccag attgatgccc atttccgata
     2041 tcatcgccat ggtatcctcg atctgctccg aggacacctg ctccgagggc agcgcggcgt
     2101 tgagctcgtc gtaggtgatg taaccgcgtt cgcggccctt ggcgatcagg cgcttgactc
     2161 cggcgccgac cgtatccagg agcgggccgt cgccctgctc ctcgcgcgac tcgccctctt
     2221 ccgccgccgc tgcttgcttc gacatacgcc tctgttctct cgaaacgaca atgccttgcc
     2281 gccccgcggc cggagccgcg gcctggtccg ctgtcaaggc aaactagtta agtggtgcct
     2341 ggcggcggcg aagcaagacc cgcctccgcc cgccccggcg acgcctcccc gtgtccccga
     2401 tcaagcgact caagttgatg ctgcaaagcg gccaaatgcg caagcgcttc gtcggtcatc
     2461 tgttgggcga gggccgcgac cgcgtccgcg atctcggcct cgagcagcgc ccggcggtgg
     2521 cgggcggcga ggtggcggaa gccgcgcagc gcctccgcct cgtgcgcccc ggggcgggcg
     2581 aaggactcgg tcaccggggc ggtggcgaag cctgagcgcc ggcacagccg cgccgccgcc
     2641 tcgcccaggc catgcgcgcg caggtagccg tcgagccgct cgtgctcgct ctgcgcgtca
     2701 ccctcggccg ccgcgatcag cgccgcgcgc agcacctcgc agtcgccggc ggcaaagctc
     2761 agcgtcgcca gctcctcgat cacctctgcg atcagcgccg ggtggttgag cagggtgacc
     2821 agcatcagcc gctcgcgctg ctcgccctca gccggcccgg cgatcttggc gctatgcccg
     2881 tgatacgcgc tgtggggtcg gagcacgtgg tggccgcgct cagccttgcg ccccgcgcgc
     2941 ggcgcgccga atgccgcctc gaagcggcgg cggaactcgg cccggtactg gtcgcgcaca
     3001 tcgcggtcgg cgatctccgc ggccagctcg tcgagccgct tacgcaggcc ggcgcgccgc
     3061 tcgggcgtgt cggcggcgcc ggcggcggcc tggcgccaga tcacctcgac cagcggccag
     3121 gcgcgctcga ggcagccggc aaccgccgcg ggcccctggc ggcgcaccag gctgtcgggg
     3181 tcgtcgccct cgggcaggaa ggcgaagcgg agtgagcgcc cgggcccgag cagcggcagc
     3241 gcgcgcgccg ccgcccgcgc cgccgcgcgc cgcccggcag tgtcgccgtc gaggcacagc
     3301 accggctcgg gcgcaagccg ccacagcgcc gccagctggt cctcggtcag cgcggtgccg
     3361 agcggcgcga ccgcgttgtc gaagcccgca gccgaaagcg cgatcacgtc catgtagccc
     3421 tcgacgacga tcacggtgcc ggcctcgcgc gccggcacgc gcgccccggc caggttgtag
     3481 agcgtgcggc ccttgcggaa caccggccct tcgggcgagt tgaggtattt gggctggccg
     3541 tcgccgagca cgcgcgcgcc gaagccgatc acccggccgc ggccgtcggc gatcgggaac
     3601 atcagccggt tgcggaagaa gtcgaacgat tcgcccgccc gctccgcgcg cctgatcagg
     3661 ccggcatcga gcgccagcgc ctcgtcgatg ccctcgccgg cgagcagcga ggcgagcgcc
     3721 cgcccgcgcc cgggcgcgta gccgagccgg aagcgcgcct gcgccggcgc gtccaccccg
     3781 cgcgagtcca ggtaggcgcg cgcggctgcg ccgtcgtcgc gcgcgagcgc gcgcgcgaac
     3841 cagtcggcgg ccttgtccag gacctcgagc aggctcgcct cgcgcgccgc ccgcgcgcgc
     3901 gcctccggtg tcggcttcgg caccgacagg ccggcctcgc ccgccagccg ctcgaccgcc
     3961 tccgggaagg ggaggccgtc gtgctgcatg acgaagccga tgatgtcgcc gtgcgcgccg
     4021 cagccgaagc agtggaagaa cgccttgtcg tcgttgaccg tgaacgacgg cgtcttctcg
     4081 ttgtggaacg ggcacagccc gacatgctcg cggccgcggc gggccagcct gaccgcgcgc
     4141 ccgatcacct cgctcgccgg caggcgcgtg cgcagctcgt cgaggaactg gggcggcagg
     4201 gccatcggcg gcgcggcgcg ctcaggcctt gccggccaag cggtccttgg cgagcgcgcc
     4261 ggcgcgggcg aagtccatgc gcccggcgta gcgctccttg agcgcggcca tcacccggcc
     4321 gacgtccttg agcgagtgcg catccaccgc ggcgatcgcc tcggcgatcg cccgcgcggt
     4381 ctccgcctcg tcgagcggct tcggcatgta gccttcgatc accgcgatct cctcggcctc
     4441 gcgctcggcc aggtcggcgc gcccgccctg gcggtagatg tcgatcgaat cgcggcgctg
     4501 gcgcaccatc ttggccagca tgtcgaggat ctggtcggcg ccgatgccgt cctcgttgcc
     4561 gtccgcgcgc accgcgatgt cgcggtcctt gagcgtcgcc aggatcaggc ggagcgtcgc
     4621 cacccggcag gtatccttcg ctttcatggc cgccttcagg tcggcggaga actgcgcacg
     4681 caaatccgac atgctttccc gctcgcggct cggttggtga tccgcccggc gcgctcggcg
     4741 gcccggcgcg atggccgagt aagcgtggag gaacgccgca tgcctgtcca ttcacaattc
     4801 cccttcagca caagttgttg ttatacaaag cgaaacactt tcggagcccg gcttgacgca
     4861 gccccggcat gtgcgtattt tccgcccgac ttgcccgccg ctccgccgct ggccgcgtgg
     4921 cctggtagga cgatccgctg acatgcccga gactcccgcg atcgccccgt cgagcgcccg
     4981 gcgctggccg acggcagtgc tggtgctggc cgacgggcgg gtgattcgcg gccgcggcct
     5041 gggagctgag gccaaggcgg tcggcgaggt gtgcttcaac acctcgatga ccggctatca
     5101 ggagatcctg accgacccgt cctacgcggc gcagatcatc accttcacct tcccgcacat
     5161 cggcaatgtc ggggcgaacg ccgaggacat cgaatcgctg accccggcgg cgcgcggctg
     5221 cgtgctgtgc gccgacatca ccgagccgtc gaactggcgc gcggcgcagc acctcgacgg
     5281 ctggctcgcc tcccacggcc tgccggcgat cgccggggtg gacacgcgcg cgctgaccca
     5341 cctgatccgc gccgccggcg cgccgagcgg cgtgctggtc cacgacgccg agggacgctt
     5401 cgatgtcgac gccttgcgcg ccgaggccgc ggcctggccc gggctcgaag gcatggacct
     5461 cgcgcgcgag gtgagctgca cccagactta cggctgggag cagacgcggt ggcggatcgg
     5521 caccggctac ggcaccctcg ccaacccccg ccggcacgtg gtcgcgatcg actacggcgc
     5581 caagcgcaac atcctgcgct gtctcgccga cgccggctgc cgggtgacgg tggtaccggc
     5641 ggcagcgagc gcggacgagg tgctgcgcca cgcgccggac ggcatcttcc tgtccaacgg
     5701 cccgggcgat ccggcggcga ccggcgccta cgcggtgccg gtgatccgcg ccttgctgga
     5761 gagcgggatt cctttgttcg gcatctgcct cggccatcag ttgctggcga tcgcgctcgg
     5821 cgcgcgcacg cgcaa
//
LOCUS       DASWHU010000392         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3583393, whole genome shotgun sequence.
ACCESSION   DASWHU010000392 DASWHU010000000
VERSION     DASWHU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583393"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VGB88_14535"
     CDS             <1..591
                     /locus_tag="VGB88_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX9648720.1"
                     /translation="GRAGCRLDVAARLRPQASGPDLVLAFAPVRHLRLDYLAEKATEL
                     GVAALAPVTTRRTVPARVNVARMRANAIEAAEQCGRLTVPEILEPAPLAEFLDRIEGR
                     RSLFWADEGGSGEAALAAFAAAGPGPAALLVGPEGGFDDDERARLAAPAGAAAFDLGP
                     RILRAETAALAALALWQAACGDWRAGAEGATRAHPA"
     gene            646..2007
                     /locus_tag="VGB88_14540"
     CDS             646..2007
                     /locus_tag="VGB88_14540"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9648721.1"
                     /translation="MAVVTQSRATVASRDELVAYIEQGCKPRETWRIGTEHEKIGFHV
                     ADLTPIPYEGARGVRAMLDGLTRFGWAPIIEGDHPIALQLDGQRITLEPGGQFELSGA
                     PLETLHQTCGEVNGHLCQVREVAEALGIGFLGIGMQPKWRRDQIPVMPKRRYDIMRAY
                     MPKVGTLGLDMMLRTCTVQANLDFESEADMVRKFRVSLALQPLAIALFANSPFTDGKP
                     NGWLSYRAHVWSDTDPARCGIPGFVFEPGMGFEAYTDFALDVPMYFVRRAGRYIDAAG
                     QSFRDFLAGRLPALPGERPSLSDWEDHLTTIFTDVRLKRYLEMRGADGGPWRMLCALP
                     ALWVGLLYDAAAIDAAQAVVADWRPDEIVALRADAARSGLAARIRGRGLREIAREVLE
                     ISRRGLSRRACLDRGGADETGFLTFLDGVVESGRTPAEAMLEAYHGRWHESVDPAFTE
                     FAY"
     gene            complement(2021..>2351)
                     /locus_tag="VGB88_14545"
     CDS             complement(2021..>2351)
                     /locus_tag="VGB88_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9648722.1"
                     /translation="LRIDTAARAWRRTSIPGIAHKVLLNEHDGPDGPLMVRLMRFRPG
                     SERPAHDCHHGEEILVLDGELADELGRYGPGTWLRTPPGSRDGARSAKGCLLYVRNGG
                     LGLALGS"
BASE COUNT          298 a          858 c          877 g          318 t
ORIGIN      
        1 gggcgcgccg gctgccggct cgacgtggcc gccaggctgc gcccgcaggc gagcgggccg
       61 gacctggtgc tggcgttcgc cccggtcagg cacttgcgcc tcgattacct ggccgagaag
      121 gcgaccgagc tcggcgtcgc cgcgctggcg ccggtgacca cgcgccgcac cgtgccggcg
      181 cgggtcaatg tcgcccggat gcgcgccaac gccatcgagg ccgccgagca gtgcggccgg
      241 ctgaccgtgc ccgagatcct ggagccggcg ccgctggctg agttcctcga ccgcatcgag
      301 ggtcgccgca gcctgttctg ggccgacgag ggcggctcgg gcgaggcggc gctcgccgcc
      361 ttcgccgccg ccggccccgg cccggcggcg ctgctggtcg ggcccgaggg cggcttcgac
      421 gacgacgagc gggcacgcct cgcggcgccg gcgggggcgg cggcgttcga cctcggcccg
      481 cgcatcctgc gcgccgagac cgcggcgctc gccgcactcg ccctgtggca ggcggcgtgc
      541 ggcgactggc gcgccggcgc agagggcgcg acaagggcgc acccggcgta gtatgagagc
      601 gtccggccgc tcggcggccg caccaccgta acgccgggag gatgcatggc cgtcgtcacc
      661 cagagcaggg ccacagtggc gagccgggac gagctggtcg cgtacatcga gcagggctgc
      721 aagccgcgcg agacgtggcg catcggcacc gagcacgaga agatcggctt tcacgtcgcg
      781 gacctgacgc cgatccccta tgagggcgcg cgcggggtgc gcgccatgct cgacgggctg
      841 acccggttcg gctgggcgcc gatcatcgag ggcgaccacc cgatcgcgct gcagctggac
      901 ggccagcgga tcaccctcga gccgggcggc cagttcgagc tgtcgggggc gccgctcgag
      961 accctgcacc agacctgcgg cgaggtgaac ggccacctgt gccaggtgcg cgaggtcgcc
     1021 gaggcgctcg gcatcggctt cctcggcatc ggcatgcagc ccaagtggcg ccgcgaccag
     1081 atcccggtga tgcccaagcg ccgctacgac atcatgcgcg cctatatgcc caaggtcggc
     1141 acgctcgggc tcgacatgat gctgcgcacc tgcaccgtgc aggccaatct cgacttcgag
     1201 tccgaggccg acatggtgcg caagttccgc gtctcgctcg cgctccagcc gctggcgatc
     1261 gcgctgttcg ccaactcgcc gttcaccgac ggcaagccca acggctggct cagctaccgc
     1321 gcccatgtat ggagcgacac cgaccccgcg cgctgcggca tcccggggtt cgtgttcgag
     1381 cccggaatgg gctttgaggc ctataccgac ttcgcgctcg acgtgccgat gtacttcgtg
     1441 cgccgcgcgg ggcgctacat cgacgccgcc ggccagtcgt tccgcgactt cctcgccggc
     1501 cggctgccgg cgctgcccgg cgagcggccg agcttgagcg actgggagga ccacctcacc
     1561 accatcttca ccgacgtccg cctcaagcgc tacctggaga tgcgcggcgc cgacggcggg
     1621 ccgtggcgga tgctgtgcgc gctgccggcg ttgtgggtgg gcctgcttta cgacgcggct
     1681 gcgatcgacg ccgcgcaggc cgtggtcgcc gactggcgtc ccgacgagat cgtcgccttg
     1741 cgggccgatg ccgcgcgctc ggggttggcg gcgcgcatcc gcgggcgcgg cttgcgcgag
     1801 atcgcgcgcg aggtgctgga gatcagccgc cggggcttga gccggcgcgc ctgcctcgac
     1861 cgcggcggcg ccgacgagac cggatttctc accttcctcg acggcgtcgt cgagtccggg
     1921 cgcacccccg ccgaggcgat gctcgaggcc tatcacggcc gctggcacga gagcgtcgat
     1981 ccggccttca ccgagttcgc ctactgaggg ccggcggggg ctaagagccg agggcgaggc
     2041 cgaggccgcc gttgcggaca tagaggagac agcccttggc gctgcgcgcg ccgtcgcggc
     2101 tgccgggcgg ggtgcgcagc caggtgccgg ggccgtagcg gcccagctcg tcggcgagct
     2161 cgccgtcgag caccaggatc tcctcgccgt ggtggcagtc gtgcgcgggc cgctcgctcc
     2221 ccggcctgaa cctcatcagc cgcaccatca gcgggccgtc cggcccatca tgctcgttga
     2281 ggagcacctt gtgggcgatg cccggaatgc tggtgcgccg ccaggcgcgc gccgcggtgt
     2341 cgatgcgcag c
//
LOCUS       DASWHU010000393         7171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2510491, whole genome shotgun sequence.
ACCESSION   DASWHU010000393 DASWHU010000000
VERSION     DASWHU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510491"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGB88_14550"
     CDS             complement(<1..188)
                     /locus_tag="VGB88_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648723.1"
                     /translation="MIEDRPGTAPPSEVLSRPVAAGSRPEVVAGMNEVPAAAKIGPDA
                     ITAKVAARLAARVHDRAA"
     gene            516..1913
                     /locus_tag="VGB88_14555"
     CDS             516..1913
                     /locus_tag="VGB88_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9648724.1"
                     /translation="MGDEPRSMVARALGLARLILRDADPARLPAWQAGPLRLVRILAA
                     VLRDLVQGQVSLRAMSLVYTTLLSLVPVLAISFSVLKGFGVHNQMQPLLLNLLSPLGE
                     QGEEITARVIGFVENVNVGVLGSVGLAVLVLTVVSLMQKIERAFNDAWRITRHRPFAQ
                     QFSSYVTVIVIGPVLVFSALGITASLVSAPVVSDLAAIAPFGLLFGVVGRVLPFLLIV
                     TAFTFLYAFLPNTRVRVAAALAGGVIAGVLWESVGWVFASFVVTSTRYTAVYSAFATL
                     ILFLIWLYLSWLILLVGVRIAFYVQNPQYVAVLPGAAALGAELRERLGLAVIRAIGRA
                     YYGLDGGAARIEALAAALGVPNDAVESVMSALERARLVVAADGAGASYLPASPPEETP
                     LKRVLEALRAGGDADSAGLRALAGDRATAAVMARLDAAAASALGATTVKDLAHDLPGA
                     GIELAPRPRQAVSRA"
     gene            1947..2444
                     /locus_tag="VGB88_14560"
     CDS             1947..2444
                     /locus_tag="VGB88_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX9648725.1"
                     /translation="MSLRRLVAGLCLAGALAALAGCSRFADAIPPEVHMADLRPAGGG
                     LFEQQFVVELRVVNPNDFPIDVEGMTFDLALNGQPFAKGLTNQSVTVPRLGEARVPVA
                     ASTNVLDLARQLLGLAGSGDLSYRISGVAYLAKGLGVDAVPYEQAGSLGLSSLTGGGN
                     ALVPR"
     gene            2425..2964
                     /locus_tag="VGB88_14565"
     CDS             2425..2964
                     /locus_tag="VGB88_14565"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor carrier protein"
                     /protein_id="HEX9648726.1"
                     /translation="MRWCRAEQRRARPVIAVMGSGIDGHGARAGPLGRWIAGQGFHLL
                     TGGGGGVMASVAEAFAAAPGRAGLVIGVLPGQAGDAYPNPWVEIVIRTHLPLTGARGA
                     EAGSRNHINVLSADVVVALPGGEGTASEVRLACAYRRPVVAHIADRGDIPGMPEQVPL
                     APGLDEVAAFVLAALDSAG"
     gene            complement(2999..3250)
                     /locus_tag="VGB88_14570"
     CDS             complement(2999..3250)
                     /locus_tag="VGB88_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648727.1"
                     /translation="MPRLNPATARNNEETLREARELGPLARIEIVEAAGDEPQHRCAA
                     ADAVRERRFAVEEVPSLPLPGCTAEDCECTYVLGTSDAI"
     gene            complement(3269..4279)
                     /locus_tag="VGB88_14575"
     CDS             complement(3269..4279)
                     /locus_tag="VGB88_14575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEX9648728.1"
                     /translation="MHRDERGRCRLSRRAFLAGSAAGGALVAAGCDGGAKLALPFEET
                     APTRPPAGEGRARTEGGEIWYQVVGSGPGTPLIVIHGGPGFSHDYLLPLAELGRDRPV
                     VFYDQLDCGKSDQPGDPGNWRVGRFVAEIESLRQALGLTRFHLLGHAWGGLLAVEYAA
                     GNRRRVDGCVLASPLISTEAWAADTRRWRRLLPDQVQAVLDREEAARNFRSQDYRQAL
                     DVFYRRHLCRLDPWPAPLTASLDKGNSRLYIAMWGETEFAATGTLRGYDASPSLTRLE
                     VPTLFTCGEFDEVAPVTVYRYSAVVADSDVRMIADASHMAHLEQTAAYNQAVAVFLNG
                     LG"
     gene            4495..5058
                     /locus_tag="VGB88_14580"
     CDS             4495..5058
                     /locus_tag="VGB88_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9648729.1"
                     /translation="MTVALESCPALVLNADYRPLSYFPLSLWPWQEVVKAVFLERVNI
                     VAEYDREVRSPSWRMRLPSVISLKAYVTSTRRPAFTRFNVFLRDGFRCQYCLARYRSE
                     DLTFDHLVPRSLGGITVWENVVTACGACNLQKGNRTASESGVAPRTRPRVPTTWELQH
                     MGRQFPPNFLHESWRDYLYWDTELDSD"
     gene            complement(5066..5554)
                     /locus_tag="VGB88_14585"
     CDS             complement(5066..5554)
                     /locus_tag="VGB88_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX9648730.1"
                     /translation="MIRVDHAGEVGAARIYAGQLAVLGHTPAGPAIRAMAAQEHAHLA
                     TFERLMVARRVRPTVLQPVWHAAGFALGAAGALLGARAAMACTVAVEEVIDEHYRRQA
                     ERLGADEPELRATIESARADELAHRDTGLAHGAAGAPARTLLEPAVKAATRLAIWLSE
                     RL"
     gene            complement(5687..6268)
                     /locus_tag="VGB88_14590"
     CDS             complement(5687..6268)
                     /locus_tag="VGB88_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX9648731.1"
                     /translation="MLTRLYAGVMALARHRHALPWLAAVSFIESSVFPIPPDVMLIPM
                     VLAERRKALLIALVCTVASVLGGIAGYAIGYGLWETVGRPLIEFYGYGEQMARFTARY
                     NEWGAWIVFGAGLTPFPYKVITIASGVAGLDPAVFIVASVLSRGLRFFVVAALLYRFG
                     APIERFIARNLGLLTVLFVVLLFAGYLVIELWR"
     gene            6419..6505
                     /locus_tag="VGB88_14595"
     tRNA            6419..6505
                     /locus_tag="VGB88_14595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6453..6455,aa:Leu,seq:caa)
     gene            6543..6779
                     /locus_tag="VGB88_14600"
     CDS             6543..6779
                     /locus_tag="VGB88_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9648732.1"
                     /translation="MKFYSFQIVIEKEPEDEGYLAYSPTLPGCFSNGATIEEAKRNIR
                     EAIELHLSALLERAAPVPQNDRLVHVEELTVGIP"
BASE COUNT          950 a         2618 c         2562 g         1041 t
ORIGIN      
        1 tccgcggcgc gatcatgaac ccgcgcggcg agccgcgcgg caactttcgc ggtgatcgcg
       61 tccggcccga ttttagccgc ggccgggact tcgttcatcc ccgcaacgac ttccggccgg
      121 ctgccggccg ccaccggccg ggacagaact tcgcttggcg gggccgtccc gggccgatct
      181 tcaatcatgc cccgcgccgc cggccgcccg caatccaagg ctttgcgccg cggtcgccgc
      241 ggtcgcagtg ggcgtggcag aaccgcgcgc accgggtgtg gcccgggcgc ccgcagctcc
      301 accaccagcg ccagttcggc tggcacgcgg tcccgccgcg gcagcggatg atgtgggacc
      361 cgcgccggca gcgcagattc tagcctgacc cgaccggcgc gtcagcgccg ccccggcccg
      421 gcgtgtgcgc cctccccgcg cacgccgggc cctctgcgcg cctggcccaa ggcctttcat
      481 gctagactcg cccgctcgat tgcgcgggag gcggcatggg cgacgagccg agatcgatgg
      541 tcgcgcgcgc gctggggctc gcgcggctga tcctgcgcga tgccgaccct gcccgcctgc
      601 cggcctggca ggcggggccg ctgcgcctgg tgcgcatcct ggcggcggtg ctgcgcgacc
      661 tcgtccaagg ccaggtctcg ctccgcgcga tgagcctggt ctacaccacc ctcttgtcgc
      721 tggttccggt gctggcgatc agcttctcgg tgctcaaggg cttcggcgtc cacaatcaga
      781 tgcagccgct gctgctcaac ctgctcagcc cgctcggcga gcagggcgag gagatcaccg
      841 cacgcgtcat cggcttcgtc gagaacgtca atgtcggcgt gctcggctcg gtcggcctcg
      901 ccgtcctggt gctgaccgtc gtgtcgctga tgcagaagat cgagcgcgcg ttcaacgacg
      961 cctggcggat cacgcgccac cgcccgttcg cgcagcagtt ctcgagctac gtgaccgtga
     1021 tcgtgatcgg gccggtgctg gtgttctcgg cgctcggcat caccgcctcg ctggtctcgg
     1081 cgccggtggt cagcgacctg gcggcgatcg cgccgttcgg cctgctgttc ggcgttgtcg
     1141 gccgcgtgct gccgttcctg ctgatcgtca ccgcgttcac gttcctgtac gccttcctgc
     1201 ccaacacgcg ggtgcgggtc gcggcggcgc tggccggggg cgtgatcgcc ggggtgttgt
     1261 gggaatcggt cggctgggtg ttcgcctcgt tcgtcgtcac ctccacccgc tacaccgccg
     1321 tctactcggc attcgcgacc ctgatcctgt tcctgatctg gctgtatctg agctggctga
     1381 tcctgctggt cggggtcagg atcgcgttct acgtccagaa cccgcaatac gtcgccgtgc
     1441 tgccgggcgc cgcggcgctc ggcgccgagc tcagggagcg gctggggctt gccgtgatac
     1501 gggcgatcgg gcgcgcctat tatgggctcg acggcggcgc ggcgcggatc gaggcgctgg
     1561 cggcggcgct cggcgtgccg aacgatgcgg tcgagtcggt gatgtcggcg ctcgagcggg
     1621 cgcggctggt ggtcgcggcc gatggcgccg gcgccagcta cctgccggcg tcgccgccgg
     1681 aggagacgcc gctcaagcgg gtgctcgaag cgctgcgcgc cggcggcgac gcggactcgg
     1741 ccgggctgcg cgccctcgcc ggcgaccgcg ccaccgccgc ggtgatggcg cgcctcgatg
     1801 ccgccgccgc aagcgcgctc ggcgcgacca cggtcaagga cctcgcccac gacctgccgg
     1861 gcgccgggat cgagcttgcg ccccgcccgc gccaggcggt cagccgcgcc tgaccccgcc
     1921 cgcccccgaa caagaggaga gtcgagatgt cgctccgccg cctcgtcgcc gggctgtgcc
     1981 tggcaggtgc gctcgccgcg ctcgccggct gcagccggtt cgccgacgcc atcccgcccg
     2041 aggtccacat ggccgacctg cgccccgccg gcggcggcct gttcgagcag cagttcgtgg
     2101 tcgagctcag ggtggtcaac ccgaacgact tcccgatcga cgtcgagggc atgaccttcg
     2161 acctggcgct caacggccag ccgttcgcca aggggctcac caaccagtcg gtgaccgtgc
     2221 cgcggctggg cgaggcccgg gtgccggtcg cggcctcgac caacgtcctc gacctcgccc
     2281 gccagctgct cggcctcgcc ggctccggcg acctgtccta ccggatcagc ggcgtcgcct
     2341 acctggccaa gggcctcggc gtcgatgcgg tgccctacga gcaggcgggc agcctcgggc
     2401 tgtcgagcct gaccgggggc ggcaatgcgc tggtgccgcg ctgagcagcg gcgcgcgcgc
     2461 ccggtcatcg ccgtcatggg gtcgggcatc gacggccacg gcgcgcgcgc cggcccgctc
     2521 gggcgctgga tcgcgggcca ggggttccat ctgctgaccg ggggcggcgg cggcgtgatg
     2581 gcgtcggtgg cggaggcgtt cgccgccgcg cccgggcgcg ccggcctggt catcggcgta
     2641 ttgccgggac aggcaggcga cgcttacccc aacccgtggg tcgagatcgt catccgcacc
     2701 catctgccgc tcaccggcgc gcgtggggcc gaggccggct cgcgcaacca catcaacgtg
     2761 ctcagcgccg acgtcgtggt cgcgctgccg ggcggggagg gcaccgccag cgaggtccgg
     2821 ctcgcctgcg cctatcgccg cccggtcgtc gcccacatcg ccgaccgcgg cgacatcccg
     2881 ggcatgcccg agcaggtgcc gctcgcgccc ggcctggatg aggtcgccgc gttcgtcctc
     2941 gccgcgctgg attccgccgg ctaatcattg gccgactagc ggtagccgcc gtccccgctc
     3001 agatcgcgtc cgaggtgccg agcacgtagg tgcactcgca gtcctcggcg gtgcagccgg
     3061 gaagcggcag cgacggcacc tcctcgaccg cgaacctgcg ctcgcgcacc gcatccgccg
     3121 ccgcgcagcg gtgctgcggc tcgtcgcccg cggcctcgac gatctcgatg cgggcgagcg
     3181 gcccgagctc gcgcgcctcg cgcagcgtct cctcgttgtt gcgcgccgtg gcgggattga
     3241 gtctcggcat ctctcgctcc ggtggcgctc agccgaggcc gttcaggaac accgccaccg
     3301 cctgattgta ggcggcggtc tgctcgagat gcgccatgtg cgaggcatcc gcgatcatgc
     3361 gcacgtccga gtccgcgacc acggcgctgt agcgatagac ggtcaccggc gccacctcgt
     3421 cgaactcgcc gcaggtgaac agggtcggca cctcgaggcg ggtgaggctc gggctcgcgt
     3481 cgtagccgcg caaggtcccg gtggccgcga actcggtctc gccccacatg gcgatgtaga
     3541 ggcgcgagtt gcccttgtcc agcgacgcgg tgagcggcgc cggccacggg tcgagccggc
     3601 acagatgccg ccggtagaac acgtcgagcg cctgccggta gtcctggctc cggaagttgc
     3661 gcgcggcctc ctcgcggtcg agcaccgcct gcacctggtc cggcagcagc cggcgccagc
     3721 gccgggtgtc ggcggcccag gcctcggtgc tgatcagcgg gctcgccagc acgcagccgt
     3781 cgacccggcg ccggttgccg gcggcgtact cgaccgccag cagcccgccc caggcgtggc
     3841 cgagcagatg gaagcgggtc agcccgagcg cctggcgcag cgactcgatc tcggcgacga
     3901 agcgccccac ccgccagttg ccgggatcgc ccggttggtc cgacttgccg cagtcgagct
     3961 ggtcgtagaa caccaccggg cggtcgcggc cgagttcggc cagcggcagc aaatagtcgt
     4021 ggctgaagcc gggcccgcca tggatcacga tcaacggcgt gccgggaccg ctgccgacca
     4081 cctgatacca gatctcgccg ccctcggtgc gggcgcggcc ctcgcctgcg ggcggacggg
     4141 tgggcgcggt ctcctcgaac ggcagcgcga gcttcgcgcc gccgtcgcag ccggcggcga
     4201 cgagcgcacc gccggcggca ctgccggcga ggaaggcgcg tcggctcagc cggcatcggc
     4261 cgcgctcgtc ccggtgcatg attgccccgc ccgttggtgc ttctacccac cctatgcgcc
     4321 gtcccccagg ccccgtcaag cccttggccg cgccgagaaa gccggcggaa acagaagctt
     4381 aaccgaagcg gcggttgccc cgcgccggga tgcgttctac actcggcgcc ggcgtgcgcg
     4441 cctcggggag gccggcgcgg gcgcgccggg cttatgtgga gatcgaccgc cgccgtgacc
     4501 gtcgcgctcg agtcctgtcc cgcgctggtg ctgaacgccg actaccggcc gctgagctac
     4561 ttcccgctgt cgctgtggcc gtggcaggag gtggtcaagg cggtgttcct ggagcgggtc
     4621 aacatcgtcg ccgagtacga ccgcgaggtg cgttcgccgt cgtggcgcat gcgcctgccg
     4681 agcgtgatct cgctcaaggc ctatgtgacc agcacccgcc ggcccgcctt tacccggttc
     4741 aacgtgttcc tgcgcgacgg cttccgctgc cagtactgcc tggcgcgcta ccgctcggag
     4801 gacctgacct tcgaccacct ggtgccgcgc tcgctcggcg gcatcacggt gtgggagaac
     4861 gtggtcaccg cctgcggcgc gtgcaacctg cagaagggca accgcaccgc gagcgaaagc
     4921 ggcgtcgcgc cgcgcacccg cccgcgcgtg ccgaccacct gggagctgca gcatatgggc
     4981 cgccagttcc cgcccaactt cctgcacgag agctggcgcg actacctgta ctgggacacc
     5041 gagctcgaca gcgactgagc gccgctcaca gccgctccga cagccagatg gcgagccggg
     5101 tcgcggcctt gaccgcgggc tcgagcagcg tccgcgcggg cgcgcccgcc gcgccgtggg
     5161 cgaggcccgt gtcgcggtgg gcgagctcgt cggcgcgcgc ggactcgatg gtcgcgcgca
     5221 actcgggctc gtccgcgccg aggcgttccg cctggcggcg atagtgctcg tcgatcacct
     5281 cctcgaccgc gaccgtgcac gccatcgccg cgcgcgcacc caacagagcg cccgccgcgc
     5341 cgagcgcgaa gccggccgcg tgccacaccg gctggagcac ggtcgggcgg acacggcgcg
     5401 cgaccatcag ccgctcgaag gtggcgagat gggcgtgctc ctgcgcggcc atggcgcgga
     5461 tcgccggccc ggcgggcgtg tgcccgagca ccgcgagctg gccggcatag atgcgtgcgg
     5521 cgccgacctc gccggcgtgg tcgacccgga tcatgcgcgc gagctgggcg tcgcgcgaca
     5581 ggtcgccggg caggcccccg agtttaaccc gggggtcgcc gagcgcgctc gtggccgcac
     5641 ctcgtggcgc gccggtcatc gaccccggtc tagcacccgg gccccctcag cgccacagct
     5701 cgatgaccag gtagccggcg aacaacagca ccacgaacag caccgtgagc agccccaggt
     5761 tgcgcgcgat gaagcgctcg atcggcgcgc cgaaccggta caggagcgcc gcgacgacga
     5821 agaagcgcag gccccgcgac agcaccgagg cgacgatgaa caccgccggg tcgagcccgg
     5881 cgacgccgct cgcgatggtg atcaccttgt aggggaacgg ggtcagcccg gcgccgaaca
     5941 cgatccatgc cccccattcg ttgtaccggg cggtgaaccg cgccatctgc tcgccgtagc
     6001 cgtagaactc gatcagcggc cggcccaccg tctcccacag cccgtagccg atggcgtagc
     6061 cggcgatgcc gccgagcacc gaggcgacgg tgcagaccag cgcgatcagc agcgccttgc
     6121 ggcgctcggc gagcaccatc gggatcagca tcacgtcggg cgggatcggg aacaccgagc
     6181 tctcgatgaa cgacaccgcg gcgagccagg ggagcgcgtg gcggtggcgg gcgagcgcca
     6241 tgacgccggc atacaggcgg gtcagcattg cggcgcgaga gcggcggcgg cgggcacggg
     6301 cagggctccg aggggcggga cgccgctctc ttaagccgag cgccgcgccg cgcgcaagcc
     6361 cgccgccatt gaccgcatcc ggcccgcggc taatatgccc gccgcggcgc ggccacgggc
     6421 ccctgtggcg gaaccggtag acgcggcaga ctcaaaatct gttgccggca acggcgtggg
     6481 agttcgagtc tccccggggg caccagggcg cggcggcggc tgaacgggag cgatgtagcc
     6541 cgatgaagtt ttattccttc cagatcgtca tcgagaagga gccggaggac gagggctatc
     6601 tggcctacag cccgaccttg ccgggctgct tcagcaatgg cgccaccatc gaggaagcca
     6661 agcgcaatat ccgggaggcg atcgagctgc acctgtcggc tttgctcgaa cgcgccgcac
     6721 cggtgcccca gaacgaccgc ctcgttcacg tcgaggagct gaccgtcggg ataccctgat
     6781 ggcgcggatg ccccaggtca cggcgcgcga gctggtgcgg ttcctcaagt tgcagggttt
     6841 tgccgagcag cgacaatcgg gcagccacct gacctaattt catgccgggc gcggcttaac
     6901 ggtcaccgtc cctgtccata ccggcgtcga tatcggccgc gcgcttgcgg tgcgaatcct
     6961 gcacgacgcc ggcttctcgg tcgaggacta cttcaggctg cggtgatatg ctttataccg
     7021 gatcgcatcg ataaggactc aggctactgt tctcgtcgtc atgtatggac ggcccccctc
     7081 tggcaagggg cgagaagcgg ttttggcaaa gagtctggag cggtcatgta ttcggcctgt
     7141 tgctgcggcc tgtcgaccgc cggccccgat g
//
LOCUS       DASWHU010000394         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1031245, whole genome shotgun sequence.
ACCESSION   DASWHU010000394 DASWHU010000000
VERSION     DASWHU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031245"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VGB88_14605"
     CDS             complement(<1..696)
                     /locus_tag="VGB88_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX9648733.1"
                     /translation="MGATPEPFELHVPDAAIADLGARLARTRFPDQPPGAPWATGTDV
                     AWLRGLVDHWRESFDWRAAEARLNAFPQYKVALAGIDLHFLHVPGAGPDPCPLLLAHG
                     WPGSVVEFLELIPRLTDPARFGGDAAESFTVVAPSLPGYGLSFAPGQTRFAVAEIADC
                     FAELMTDVLGYRRYAAQGGDWGSFIVSLLAHAHPERMIGIHLNLLPLRRDPKMVADPS
                     PEERAYLDQLAGFL"
     gene            849..1106
                     /locus_tag="VGB88_14610"
     CDS             849..1106
                     /locus_tag="VGB88_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648734.1"
                     /translation="MIGPDGREVYVNGNYEDVAGGTNEIFAVEYGNNRFETLDEWFRI
                     DLRADAEVNDAQPHVESELAPVEPPEPTALTPPGAGEADDQ"
     gene            1175..2602
                     /locus_tag="VGB88_14615"
     CDS             1175..2602
                     /locus_tag="VGB88_14615"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9648735.1"
                     /translation="MGALVLALAACSYPTRNQELASLDPRQGYRWGALAPTELESTLL
                     VVTASGGGTRAAAFTLSALSGLADLELASGRSLADEIDVISSVSGGSVTAAYFALEGP
                     GGFQDLEDNFIRQDGISAILWRGLKPVELAALATPSRERIDLLIDYLDESLFHGATFD
                     SLRQGGERPLLILNAGDMVDGAPFAFTQNTFDLICSDLDRLALSVAVAASAAFPGALS
                     PVTLTNYAPCPAQQAVSWPTTWVNQAAASRWYDNPSRVRRGRDAQNYALGAAAATPAP
                     RKYIHLLDGGIADNLGIAEPFRLLSSNEVSPNFFNQISRGQIDKVVFVLVNARSDKPS
                     RLSGEIATPGALDMVQATINAAIDNTTFGNFERLETLLRERLDAAAAEFPEPLASNFR
                     KVETYFLPIDFDAIADDACRDAFQSIATSWTLAKREVDALMIAGKALLKSAPKLSRLA
                     ADLGLAGIDRLPELEEACAAIRASG"
     gene            complement(2594..>5050)
                     /locus_tag="VGB88_14620"
     CDS             complement(2594..>5050)
                     /locus_tag="VGB88_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX9648736.1"
                     /translation="GIIGELGARGTKAAIIITAGFAESGASGRLLQQAVLDAAKPHLL
                     RIVGPNCLGVMVPDSGLNASFAQASPRPGRLAFVAQSGAIVTSVLGWAEARGIGFSHL
                     ASLGDMADVDFGDMLDYLANDRGTRAILLYVEAITQARKFMSAARAAARMKPVIVVKA
                     GRQPEGARAAASHTGAMAGSDAVYDTAFRRAGMLRVYSLEALFEAVATLATAEPPGGD
                     RLAIVTNGGGVGVMATDSLIEDGGRLAELAPETIAALDQALPPTWSHGNPVDIIGDAP
                     PGRYRDALAAVLADPGVDAVLVLNCPVAVASSVDAARAVIETAGKRRKPVLLTNWLGD
                     GEAIAARRLFAEHGVPSYDTPMAAVRAFMQMVTYRRNQEMLMETPPSVPEDFTPETER
                     ATAIVARAHAEGREWLSEPEAKDVLACYAIPVARTRAARNPGEAAALAAEIGSAVVLK
                     ILSPDITHKSDVGGVALALLGPAAVEDAATAMAARIGEAMPEARIEGFTVQPMVHRPD
                     AFELIVGATVDARFGPAILFGHGGTAVEVVGDTALALPPLNMHLAREVMSRTRVWRQL
                     SGYRERPAVNLDAVALTLVKVSQLVVDIAGIEEIDINPLLADAYGVIALDARIRVGET
                     VGPAAERLAIRPYPKELEEQIPLGDGRKLLLRPVVPEDEPAFQAGFAKLTPEEVRLRF
                     FVPMKTLSHVQAARFTQIDYDREMALILTEAGRPGTTDIFGVVRISADPDNDRAEYAV
                     IVRGDMTGMGLGVLLMRRIIDYARNRGIGEIFGDVLRDNTTMLRLCDMLGFRRSSMAD
                     EPELVRVTLELRPRAGRASS"
BASE COUNT          727 a         1830 c         1787 g          706 t
ORIGIN      
        1 gaggaacccg gcgagctggt cgaggtaggc gcgctcctcg ggcgaggggt cggcgaccat
       61 cttagggtcg cggcgcagcg gcagcaggtt gagatggatg ccgatcatgc gctcgggatg
      121 cgcgtgggcg agcagcgaga cgatgaacga gccccagtcg ccgccctgcg cggcatagcg
      181 ccgatagccg agcacgtcgg tcatcagctc ggcgaagcag tcggcgatct cggcgaccgc
      241 gaagcgcgtc tggccgggcg cgaacgacag gccgtagccg ggcagcgacg gcgccaccac
      301 ggtgaacgac tcggccgcat cgccgccgaa gcgtgccggg tcggtgaggc gcgggatcag
      361 ctcgaggaac tcgaccaccg agcccggcca gccatgcgcg agcagcaacg ggcacgggtc
      421 gggcccggct cccgggacgt gcaggaagtg caggtcgatg ccggcgagcg cgactttgta
      481 ctgcgggaag gcattgagcc gcgcctcggc ggcgcgccag tcgaacgact cgcgccagtg
      541 gtcgaccagg ccgcgcagcc acgcgacgtc ggtgccggtg gcccacggcg cccccggcgg
      601 ctggtcgggg aagcgggtgc gggcgagccg cgcgccgagg tccgcgatcg ccgcgtcggg
      661 cacgtgcagc tcgaatggct ctggcgttgc gcccatgatt cctcctcggc cggcctactc
      721 cgccgaagca gccgcttcgg ctgcgaaggc tggaccggct gggtctcggc ccgtgcgtgc
      781 tgtatcatgc ccgggtcagc gaaactcggc aaaagggtgc ggcggcaatg ccttgggggt
      841 cggtcaggtt gatcgggccg gacggccgcg aggtctacgt caacggcaac tacgaggacg
      901 tggccggcgg aaccaacgag attttcgccg tcgagtacgg caacaaccgg ttcgagaccc
      961 tggacgagtg gttccgcatc gacctgcgcg cggacgccga ggtcaacgac gcgcagccgc
     1021 atgtcgagtc cgagctggcg ccggtcgagc cgcccgagcc gaccgcgctg accccgcccg
     1081 gcgccggcga ggccgacgac caatgaccgg cgggcctcgg ctatgcctac tccgccgaag
     1141 cagccgcttc ggctgcgaag gctggatcag cctcgtgggc gcgctggtcc tcgcgctcgc
     1201 cgcctgcagc tatccgaccc gcaaccagga gctcgccagc ctcgatccgc gccagggcta
     1261 ccgctgggga gcgctcgcgc cgaccgagct cgaaagcacc ctgctggtgg tcaccgcctc
     1321 gggcggcggc acgcgcgcgg ccgctttcac gctgtcggcg ctcagcggcc tcgccgatct
     1381 cgagcttgcc tcggggcgga gcctcgccga cgagatcgac gtcatctcgt cggtctcggg
     1441 cggcagcgtg accgcagcct atttcgcgct cgaagggccg ggcggcttcc aggacctgga
     1501 agacaacttc attcgccagg acggcatctc cgcgatcctg tggcgcgggc tcaaaccggt
     1561 tgagctcgcc gcgctcgcca ccccgtcgcg ggagcggatc gacctcttga tcgactatct
     1621 ggacgagagc ttgtttcacg gcgcgacctt cgactccttg cgccaaggag gcgaacggcc
     1681 gctgctgatc ctcaatgcgg gcgatatggt ggacggcgcg ccgttcgcgt tcacccagaa
     1741 taccttcgac ctgatctgct ccgacctcga ccggctcgcg ctgtcggtgg cggtcgcggc
     1801 ctcggccgcc tttcccggcg cgctgtcgcc ggtgaccttg accaactacg cgccctgccc
     1861 ggcgcaacag gcggtgtcat ggccgacgac atgggtgaac caggcggcgg cgtcgcgctg
     1921 gtacgacaac ccctcgcgcg tccggcgcgg gcgcgacgcg caaaattacg cgctcggcgc
     1981 ggcggccgcc acgcccgcgc ccaggaaata catccacctg ctcgacggcg gcatcgcgga
     2041 caacctgggc atcgccgagc cgttccgcct gctcagcagc aacgaggtgt cgcccaactt
     2101 cttcaaccag atttcgcgcg ggcaaatcga caaggtcgtc ttcgtcctgg tcaatgcccg
     2161 ctccgacaag ccctcgcgcc tgagcgggga gatcgcgacc cccggcgcgc tcgacatggt
     2221 gcaagcgacc atcaacgccg ccatcgacaa caccacgttc ggcaacttcg agcgcctgga
     2281 gaccctgctc cgggaacgcc tcgacgccgc ggcggccgag ttccccgagc cgctcgcgtc
     2341 caacttccgc aaggtcgaga cctacttcct gccgatcgac ttcgacgcca tcgccgacga
     2401 cgcgtgccgc gacgcgttcc agtcgattgc caccagctgg accttggcca agcgggaggt
     2461 cgacgccctg atgatcgcgg gcaaggcgct gctcaagtcg gcgccgaagc tcagccggct
     2521 ggccgccgac ctcggcctcg ccggcatcga ccgcctgcct gagcttgagg aagcgtgcgc
     2581 cgcgatccgg gcgtcaggat gaggcgcggc cggcgcgcgg gcgcagctcg agcgtgaccc
     2641 tgaccagctc cggctcgtcc gccatcgagg agcgccggaa gccgagcatg tcgcacagcc
     2701 tcagcatggt cgtgttgtcg cgcagcacgt cgccgaagat ctcgccgatg ccgcggttcc
     2761 gcgcgtagtc gatgatgcgg cgcatcagca acacgccgag gcccatgccg gtcatgtcgc
     2821 cgcgcacgat caccgcatac tcggcgcggt cgttgtcggg atcggcgctg atccgcacca
     2881 cgccgaagat gtcggtggtg ccggggcgcc cggcctcggt caggatcagc gccatctcgc
     2941 gatcgtagtc gatctgggtg aagcgcgccg cctggacgtg cgacagggtc ttcatcggca
     3001 cgaagaagcg cagccgcacc tcctccggcg tcagcttggc gaagcccgcc tggaacgccg
     3061 gctcgtcctc gggcaccacc ggcctgagca gcagcttgcg cccgtcgccg agcgggatct
     3121 gctcctccag ctccttcggg tagggccgga tcgcgagccg ctctgccgcc ggccccaccg
     3181 tctcgccgac gcggatgcgc gcatcgagcg cgatcacgcc gtaggcgtcg gcgagcagcg
     3241 ggttgatgtc gatctcctcg atgccggcga tgtcgaccac cagttgcgag accttgacca
     3301 aggtcagcgc gacggcgtcg agattgaccg ccgggcgctc gcggtagccg ctgagctggc
     3361 gccacacccg ggtccgcgac atcacctcgc gggcgaggtg catgttgagc ggcggcagcg
     3421 ccagcgcggt gtcgccgacc acctcgaccg cggtgccgcc gtggccgaac aggatcgcgg
     3481 gcccgaaccg ggcatcgacg gtggcgccga cgatcagctc gaaggcgtcg ggccggtgga
     3541 ccatcggctg cacggtgaag ccctcgatgc gcgcctcggg catggcctcg ccgatgcgcg
     3601 ccgccatcgc cgtcgccgcg tcctcgaccg ccgccggccc caggagcgcg agcgcgaccc
     3661 cgcccacgtc gctcttgtgg gtgatgtcgg gcgacaggat cttgagcacc accgcgctgc
     3721 cgatctcggc cgcgagcgcc gcggcctcgc ccggattgcg cgccgcgcgc gtccgcgcca
     3781 ccgggatcgc gtagcaggcg agcacgtcct tcgcctcggg ctcgctcagc cattcccggc
     3841 cctcggcgtg cgcgcgggcg acgatcgccg tcgcccgctc ggtctcgggc gtgaagtcct
     3901 cgggcaccga gggcggcgtc tccatcagca tttcttggtt gcgccgatag gtgaccatct
     3961 gcatgaacgc gcgcactgcc gccatcggcg tgtcgtagct cggcacgccg tgctcggcga
     4021 acagccgccg cgccgcgatc gcctcgccgt cgccgagcca gttggtgagc aggacaggct
     4081 tgcggcgctt gcccgcggtc tcgatcaccg cccgtgcggc gtccacgctc gaggccaccg
     4141 ccaccgggca gttgagcacc agcaccgcgt ccaccccggg atcggcgagc accgccgcca
     4201 gcgcgtcgcg gtagcgcccg ggcggcgcgt cgccgatgat gtcgaccggg ttgccgtgcg
     4261 accaggtcgg cggcagcgcc tggtccagcg ccgcgatcgt ctcgggcgca agctcggcca
     4321 gccggccgcc gtcctcgatc agggagtcgg tggccatcac gccgacgccg ccgccgttgg
     4381 tgacgatggc cagccggtcg ccgcccggcg gctcggccgt ggccaaggtc gccaccgctt
     4441 cgaacagcgc ctccaggctg tacaccctga gcatgccggc gcgccggaag gcggtgtcgt
     4501 agaccgcgtc cgagccggcc atcgcgccgg tgtgcgaggc ggcggcgcgc gcgccctcag
     4561 gctggcggcc ggccttgacc acgatcaccg gcttcatgcg cgcggcggca cgcgcggcgg
     4621 acatgaactt ccgcgcctgg gtgatcgcct cgacgtagag caggatggcg cgcgtgccgc
     4681 ggtcgttggc caggtagtcg agcatgtcgc cgaagtcgac gtcggccatg tcgccgagcg
     4741 aggccaggtg cgagaagccg atgccgcgcg cctccgccca gcccagcacc gaggtgacga
     4801 tcgcgcccga ctgggcgacg aaggcaagcc gcccgggccg cgggctggcc tgggcgaagc
     4861 tcgcattcag cccgctgtcc ggcaccatca cgccgaggca gttggggccg acgatcctga
     4921 gcaggtgcgg cttggcggcg tcgagcaccg cctgctggag gaggcggccg ctggcgccgc
     4981 tctcggcgaa gccggcggtg atgatgatcg cggccttggt gccgcgcgcg ccgagctcgc
     5041 cgatgatgcc
//
LOCUS       DASWHU010000395         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1032910, whole genome shotgun sequence.
ACCESSION   DASWHU010000395 DASWHU010000000
VERSION     DASWHU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032910"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3401)
                     /gene="mfd"
                     /locus_tag="VGB88_14625"
     CDS             complement(<1..3401)
                     /gene="mfd"
                     /locus_tag="VGB88_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9648737.1"
                     /translation="MRGLAELIAAPGRATVAGAPEGRDVLALADLRRAAPERDLMFIA
                     RDDARMSAVADALAVFAPAEPVLRLPAWDCLPYDRVSPRADLCAQRLHTLSALLRHDR
                     GAGRGVLVLTTVNAVLQRLPPRAALAEAHFAAAVGEEVDVEALSAFLARNGYQRVGTV
                     MEPGEYSVRGGIIDLFAPGADQPVRLDLFGDRLEGLRAFDPITQRTTGQRARFDLVPV
                     SEVVLDDAAIGRFRAGYRDLFGAVTRDDALYEAVSAGRRHPGVEHWLALFHDRLDTVF
                     DYLPEAAVALEHLAEPARDQRLAAIADYYEARLAARGTGSQLGNVLYNPVPPARMFLA
                     QADWGGALGARAVLAFTPFAAPAGEATVIDLGARRARDFAPERRHPDTNLFDAVCAHA
                     RAVLADGRKLVIACASAGARDRLVALLSDHGLGDIDTAAGWDGVERLAAGRAAALVLW
                     LEHGFRTPTLEVLSEQDILGDRLARPSRRPRRAANFLTEASALEGGDLVVHIDHGIGR
                     YQRLEAIEVAGAPHDCLLMLYAGGDKLYLPVENIDMLSRYGGGEAAEGVQLDRLGGAG
                     WQARKARIKERLRDMAEALIRVAAERALRTVARMAVPAGLYEEFAARFPYAETDDQRR
                     AIDDCLADLAGDKPMDRLVCGDVGFGKTEVALRAAFVATFAGNQVAVVVPTTLLCRQH
                     YQTFKERFAGLPVHLGQLSRLVPAKEASAVRQGLAEGKVDLVIGTHALLAKGVSFSAL
                     GLLVVDEEQHFGVAQKERLKQLKADVHVLTMTATPIPRTLQLAVSGVRDLSLIATPPV
                     DRLAVRTFVTPYDPVIVREALMRERYRGGQSFYICPRIEDLDKVAAELARVVPELSVA
                     IAHGRLAARRLEDVMAAFYDGKYDVLLSTSIIESGLDLPRVNTLIVHRSDMFGLSQLY
                     QLRGRIGRSKTRAYAYFTVPSEKLLKGAAEKRLHVIQTLDTLGAGFSLASHDLDIRGA
                     GNLLGEEQSGHIREVGLELYQQMLEEAVQAARRGAAGAEAPPLEEKWSPQIAVGIAVL
                     IPDNYVADLGVRLGLYRRLSDLEDERDIDAFAAELADRFGPLPGEVENLLKVVRIKLL
                     CRRAGVEKVEAGPKGALVAFRGARVASPDKLIAFIGRNADSVRLRP"
     gene            complement(3401..3676)
                     /locus_tag="VGB88_14630"
     CDS             complement(3401..3676)
                     /locus_tag="VGB88_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX9648738.1"
                     /translation="MTTPSALELRRKRLRFRCWHRGSREADLLFGRFAARYLERLDPA
                     QIGRLEALLAEEDPDVWAWVVGRAPAPPEFDHDVLALLRQAQDGPDS"
     gene            3753..>5161
                     /locus_tag="VGB88_14635"
     CDS             3753..>5161
                     /locus_tag="VGB88_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9648739.1"
                     /translation="MRPEILNPLFAPVTALPGVGRRLAAPLGRLAGPRVVDLLWHLPS
                     AVIDRRFSPKVAEAPDGAIATVTVTVIEHRPPYNPRQPYRVMCQDDSGDIALVFFHAK
                     PDYLRRILPPGEVRVVSGTVAHFAGAVQMTHPDHVVTPAEASTLPRIEPVYPLTEGIT
                     PRPLAKAIAAALARAPELPEWLDPAFKRERGWPDWRAAVAAAHAPASVADLAPETPAR
                     QRLAFDELLANQLALALVRQHLRRTGRALAGDGRLRAQAAAALPFALTASQAAAVAEI
                     AADLALPRRMLRLLQGDVGSGKTVVALMAMLIAVEAGCQAALLAPTEILARQHYDAIA
                     PLAAAAGVRAAALTGRDKGKARERLLADAAAGAIDILIGTHALIQKDVRFRDLALAVV
                     DEQHRFGVHQRIRLQAKGHGLDMLVMTATPIPRTLMLTAYGDMDVSRLTEKPPGRQPV
                     TTAALPLSRLAEVVAAVRRA"
BASE COUNT          703 a         1923 c         1885 g          650 t
ORIGIN      
        1 gggcgcaggc ggaccgaatc ggcgttgcgg ccgatgaacg cgatcagctt gtcgggggaa
       61 gccacgcgcg cgccgcggaa ggcgaccagc gcgcccttgg gcccggcctc gaccttctcg
      121 accccggcgc gccggcacaa cagcttgatg cgcaccacct tgagcaggtt ctcgacctcc
      181 cccggcaacg gcccgaagcg gtcggcgagc tcggcggcga aggcgtcgat gtcgcgctcg
      241 tcctcgaggt ccgacagccg ccggtaaagc cccaagcgca cgccgaggtc ggcgacgtag
      301 ttgtccggga tcagcaccgc gatgccgacc gcgatctgcg gcgaccactt ctcctcaagc
      361 ggcggcgcct cggcgccggc cgcgccgcgc cgcgccgcct gcaccgcctc ctcgagcatc
      421 tgctggtaga gctcgaggcc gacctcgcgg atatggccgg actgctcctc gcccaagagg
      481 ttgccggcgc cgcggatgtc gaggtcgtgg ctcgccaggc tgaacccggc gccgagggta
      541 tcgagcgtct ggatgacgtg caggcgcttc tcggccgcgc ccttcagcag cttctccgac
      601 ggcacggtga aatacgcgta ggcgcgcgtc ttcgagcggc cgatgcgccc gcgcagctgg
      661 tagagctgcg acagcccgaa catgtccgag cggtgcacga tgagcgtgtt gacccgcggc
      721 aggtcgaggc cggactcgat gatcgaggtc gacagcagca cgtcgtactt gccgtcgtag
      781 aacgcggcca tcacgtcctc gagccggcgc gcggcgagcc gtccgtgggc gatcgcgacg
      841 ctgagctcgg gcaccacccg ggcgagctcg gcggccacct tgtccaggtc ctcgatgcgc
      901 gggcagatgt agaagctctg cccgccccgg tagcgctcgc gcatcagcgc ctcgcgcacg
      961 atcaccggat cgtagggcgt gacgaaggtg cgcaccgcca gccggtcgac cggcggggtg
     1021 gcgatcaggc tcaggtcgcg cacgcccgag accgcgagct ggagcgtgcg cgggatcggg
     1081 gtcgcggtca tggtcagcac gtgcacgtcg gccttgagct gcttcaggcg ctccttttgg
     1141 gcgacgccga aatgctgttc ctcatcgacc accagcaggc cgagggcgga gaacgacacc
     1201 cccttggcga ggagcgcgtg ggtgccgatg accaggtcga ctttgccctc ggcgagcccc
     1261 tggcgcaccg ccgacgcctc cttggccggc accagccgcg acagctggcc aaggtgaacc
     1321 ggcagcccgg cgaagcgctc cttgaaggtc tggtaatgct gccggcagag cagcgtcgtc
     1381 ggcaccacca ccgccacctg attgccggcg aaggtggcga cgaacgccgc tcgcagcgcc
     1441 acctcggtct tgccgaaacc gacgtcgccg cacaccagcc ggtccatcgg cttgtccccg
     1501 gccaggtcgg cgaggcagtc gtcgatcgcg cgccgctggt cgtcggtctc ggcataggga
     1561 aagcgcgccg cgaactcctc gtacaatccg gccggcacgg ccatgcgcgc cacggtgcgc
     1621 agcgcgcgct cggccgcgac ccggatcagc gcctccgcca tgtcgcgcag gcgctccttg
     1681 attcgcgcct tgcgcgcctg ccagccggcg ccgccgaggc ggtcgagctg caccccttcg
     1741 gccgcctcgc cgccgccgta gcgcgacagc atgtcgatgt tctcgaccgg caggtacagc
     1801 ttgtcgccgc cggcatagag catcagcagg cagtcgtgcg gcgcgccggc gacctcgatc
     1861 gcctccaggc gctggtagcg gccgatgccg tggtcgatgt ggaccaccag gtcgccgcct
     1921 tcgagcgcgc tcgcctcggt caggaagttg gccgcgcgac gtggccggcg cgacgggcgg
     1981 gccaggcggt cgccgaggat gtcctgctcg gacaggacct cgagcgtcgg cgtgcggaag
     2041 ccgtgctcga gccacagcac cagcgccgcc gccctgccgg cggcaagccg ctcgaccccg
     2101 tcccagcccg ccgcagtgtc gatgtcgccc aatccgtggt cgctgagcag cgccaccagc
     2161 cggtcgcgcg cgcccgcgct cgcgcaggcg atcaccagct tgcggccgtc ggccagcacc
     2221 gcgcgggcgt gcgcgcacac ggcgtcgaac aggttggtgt ccgggtgccg gcgctcgggc
     2281 gcgaagtcgc gcgcgcgccg ggcgccgagg tcgatcacgg tcgcctcgcc cgccggcgcc
     2341 gcgaacgggg tgaaggcgag caccgcgcgc gcgccgagcg cgccgcccca gtcggcttgg
     2401 gcgaggaaca tgcgcgcggg cggcaccggg ttgtagagca cgttgccgag ctgcgagccg
     2461 gtgccgcgcg cggcaaggcg cgcctcgtag tagtcggcga tcgccgcgag ccgctggtcg
     2521 cgcgccggct cggcgaggtg ctcgagcgcc accgccgcct ccggcaagta gtcgaacacc
     2581 gtgtcgagcc ggtcgtggaa cagcgccagc cagtgctcca cgcccggatg ccggcgcccc
     2641 gccgagaccg cctcatagag cgcgtcgtcg cgggtcaccg cgccgaacag gtcgcggtag
     2701 ccggcgcgga agcggccgat cgcggcgtcg tcgagcacca cctccgacac cggcaccagg
     2761 tcgaagcgcg cgcgctggcc ggtggtccgc tgggtgatcg ggtcgaaggc gcgcaagccc
     2821 tccaggcggt cgccgaacag gtcgaggcgc accggctggt cggcgcccgg cgcgaacaaa
     2881 tcgatgatgc cgccgcgcac cgagtactcg ccgggctcca tcaccgtgcc gacgcgctgg
     2941 tagccgttgc gggcgaggaa cgccgacaat gcctcgacat cgacctcctc gccgaccgcg
     3001 gcggcgaaat gggcctccgc cagcgccgcg cgcggcggca ggcgctgcag caccgcattg
     3061 accgtggtca gcaccagcac gccgcggccc gcgccccggt cgtggcgcag cagcgcgctc
     3121 aaggtatgca ggcgctgggc gcacaggtcg gcgcgcggcg atacccggtc gtagggcagg
     3181 cagtcccacg ccggcaggcg cagcaccggc tcggcgggcg cgaacaccgc gagcgcgtcg
     3241 gcgaccgcgc tcatgcgcgc gtcgtcgcgg gcgatgaaca tgaggtcgcg ctcgggcgcg
     3301 gcgcgccgca ggtcggcgag cgccagcacg tcgcgcccct cgggcgcgcc ggcaacggtg
     3361 gcgcgcccgg gcgccgcaat cagctcggcc agcccgcgca ctacgagtcc gggccgtcct
     3421 gcgcctggcg cagcagggcc agcacgtcgt gatcgaactc gggcggcgcc ggggcgcgcc
     3481 cgaccaccca cgcccagacg tcggggtcct cctcggcgag cagcgcctcg aggcggccga
     3541 tctgggccgg atccaggcgc tccaggtagc gcgcggcgaa gcgcccgaac aacagatcgg
     3601 cctcgcgcga gccgcggtgc cagcagcgga acctgagccg cttgcggcgc agctcgaggg
     3661 cggagggggt ggtcatggcg gcaccgatat aggccgttgc gcgcgcgctg tcagccccgc
     3721 gccggggcct gattcatgat agtcagcgca tcatgcggcc ggaaatcctc aacccgctgt
     3781 tcgcgccggt cacggcgctg cccggggtcg gccggcgctt ggctgcgccg ctcggccggc
     3841 tcgccgggcc gcgggtcgtc gacctgctgt ggcacctgcc gagcgcggtc atcgaccgcc
     3901 gcttctcgcc caaggtggcc gaggcgccgg acggcgcgat cgcgacggtg accgtgaccg
     3961 tgatcgagca ccgcccgccg tacaatcccc gccagcccta ccgggtgatg tgccaggacg
     4021 acagcggcga catcgcgctg gtgttcttcc acgccaagcc cgactacctg cgccggatcc
     4081 ttccccccgg cgaggtacgc gtggtcagcg gcacggtcgc tcactttgcc ggcgcggtgc
     4141 agatgaccca ccccgaccac gtcgtcacgc cggcggaggc atcgaccttg ccccgcatcg
     4201 agccggttta tccactgacc gagggaatca cgccgcggcc gctggcgaag gcgatcgcgg
     4261 cggcgctggc gcgcgcgccc gagctgcccg aatggctcga cccggcgttc aagcgagagc
     4321 gcggctggcc ggactggcgt gcggcggtgg ccgcggcgca tgcgccggcg agcgtcgccg
     4381 acctcgcgcc cgagaccccg gcgcgccagc gcctcgcctt cgacgagctg ctggccaacc
     4441 agctcgcgct cgcgctggtg cgccagcatt tgcgccgcac gggccgcgcg ctcgccggcg
     4501 acgggcggct gcgggcgcaa gcggcggcgg cgctgccgtt cgcgctgacc gcctcgcagg
     4561 cggcggcggt ggctgagatc gcggccgacc tcgctttgcc gcgccggatg ctgcgcctgc
     4621 tccagggcga cgtcggcagc ggcaagaccg tggtcgcgct gatggcgatg ctgattgcgg
     4681 tcgaggccgg ctgccaggcg gccctgctgg cgccgaccga gatcctggcg cgccagcatt
     4741 acgacgccat cgccccgctg gcggcggcgg cgggcgtgcg cgcggcggcg ctgaccgggc
     4801 gcgacaaggg caaggcgcgc gagcgcctgc tcgccgatgc cgccgccggc gcgatcgaca
     4861 tcctgatcgg cacccacgcg ctgatccaga aggacgtgcg cttccgcgac ctcgctctgg
     4921 cggtggtcga cgagcagcac cggttcggcg tccaccagcg catccggctg caggccaagg
     4981 gccacggcct cgacatgctg gtgatgaccg cgaccccgat cccgcgcacc ctgatgctga
     5041 ccgcctatgg cgacatggac gtatcgcgcc tgaccgagaa gccgcccggc cgccagccgg
     5101 tgaccaccgc ggcgttgccg ctgtcgcgcc tcgcggaggt ggtggcggcg gtgcgccgcg
     5161 c
//
LOCUS       DASWHU010000396         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_656886, whole genome shotgun sequence.
ACCESSION   DASWHU010000396 DASWHU010000000
VERSION     DASWHU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656886"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VGB88_14640"
     CDS             complement(<1..479)
                     /locus_tag="VGB88_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9648740.1"
                     /translation="MALTFGQAAILGATGPTGRRLARELRGRGVAVRVVSRSYANLAR
                     AFDGLDVERASWDLRLPTEARRALAGCDVAFDCIGLPAEHMADHPVTARALAAAATAE
                     GTRLVQVSSYWAYLPAVMLPITEDHPRTGGSEWVRLRRKAEDILLAAGAAVAHLPDF"
     gene            complement(497..2329)
                     /gene="ftsH"
                     /locus_tag="VGB88_14645"
     CDS             complement(497..2329)
                     /gene="ftsH"
                     /locus_tag="VGB88_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017929133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9648741.1"
                     /translation="MLAIWIALGTGIDRPAPSLELPYSAFKQLVAADKVVRASFRDGD
                     IAAELVSGAGVTDPQGAAVSRIATRVPEVGDDALLPLLEQHNVEYRSLPKADGVGTWL
                     VGLLPWLMLIAVYWWFWNRTYSNLAGRFGGRDIGDFLAGSATRATKAGAPKVRFDDVA
                     GQDNAKREVAELVDFLRAPDRYTRLGAEPPRGVLLVGPPGTGKTLLARALAGEAGVPF
                     FHISASEFIEMFVGVGASRVRKMFEEAKKRAPSIIFIDELDAVGRVRGAGLGGGHDER
                     EQTLNQILSEMDGFSGHEAVIVLAATNRPDVLDPALLRPGRFDRHVTLELPDKRARAA
                     ILRVHTRKVPLGGDVDLDVIAAGTPGFSGADLKNLVNEAAMAAARDGEAAVTMRQFDA
                     MRDKLLMGSVRTLAIQPEERHRLAVHESGHTAVAHYLPTADPLYKVTIIPRGHALGGT
                     HMLPEVERHTLPEDYLKDKLAVMLGGRVAEREVLGNVSSGADDDIRQATSLARAMVAR
                     WGMSEEVGPVDLRQAEEHPFLGREMAQPRHFSEASAHAVDEAVRKLLAEAEGRARQVL
                     ADHRAEVEALIAALEAQETLERDQIVRHLGPARGRAAQVAAVAE"
     gene            complement(2448..3266)
                     /locus_tag="VGB88_14650"
     CDS             complement(2448..3266)
                     /locus_tag="VGB88_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9648742.1"
                     /translation="MYKDILVHAEEQPAGAARFAAACALARRHDARLIALFALEFAEL
                     PGYVAAQIGEEALRRAREAYLAHAARARARLEAAAGRADVHFEWREAPGAARDLLVEH
                     GRYADLIIIGQPDPEQDGARPQGFPGELALASGRPLLTIPYAGRHDDLGRRALVAWNA
                     SREATRAVHDALPLLRAAESVVVLAVDPPEQGHIPGADIAAHLAHHGVHVEARHSVAP
                     DIEVGDELLNMASDLGSDLIVMGAYGRSRLREAVFGGATRHILEHMTVPVLMAH"
     gene            complement(3451..3903)
                     /locus_tag="VGB88_14655"
     CDS             complement(3451..3903)
                     /locus_tag="VGB88_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648743.1"
                     /translation="MTSAPRRLVAPILATLAVMLGAVAAWADDAEDGREPYINTCSKC
                     HGLVNEDAVSWTPEALLNLAVTMPLGPSLTGVYDRPAGIMENYPYSRAFRAALENPWR
                     WDEDTLDGWLTSTQDFIRGTTMFAKVEDEPRAKIIAYLKRYSPYQGDQ"
     gene            4470..4763
                     /locus_tag="VGB88_14660"
     CDS             4470..4763
                     /locus_tag="VGB88_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648744.1"
                     /translation="MPGLADEPEAPVAVDGGCAWRTQAKAVICGGLVYVGAVGAAGNV
                     FASGGTLGAAVEAAVVAGGVGALIGGAVSMGLDRRPGGSAGRRIARGGKSRFV"
BASE COUNT          635 a         1714 c         1847 g          690 t
ORIGIN      
        1 tagaagtcgg gcaggtgggc gaccgccgcc ccggccgcga gcaggatgtc ctcggcctta
       61 cgccgcaggc gcacccactc gctgccgccg gtgcgcggat ggtcctcggt gatcggcagc
      121 atcacggccg gcaggtaggc ccagtagctc gacacctgga ccagccgcgt gccctcggct
      181 gtggcggccg cggcgagcgc gcgggcggtg accgggtggt cggccatgtg ctccgccggc
      241 aggccgatgc agtcgaaggc gacgtcgcag ccggcgagcg cgcgccgcgc ctctgtgggc
      301 aggcgcaggt cccaggaggc gcgctcgacg tcgaggccgt cgaacgcgcg ggcgagattg
      361 gcgtagctgc gcgagaccac ccgcaccgcg accccgcgcc cgcgcagctc gcgcgcgagc
      421 cttcgcccgg tcggccccgt cgcgccgaga atcgccgcct ggccgaaggt cagcgccatg
      481 cgcgccgcgg ccgcggctac tcggcgaccg cggcgacttg cgcggcgcgg ccccgcgccg
      541 ggcccagatg gcgcacgatc tggtcgcgct cgagcgtctc ctgcgcctcc agcgcggcga
      601 tcagcgcctc gacctcggcg cggtggtcgg cgagcacctg gcgggcgcgc ccctcggcct
      661 cggccaggag cttgcgcacc gcctcgtcga ccgcgtgcgc ggatgcctcg ctgaagtggc
      721 gcggctgggc catctcgcgg cccaggaacg ggtgctcttc cgcctggcgc aggtcgaccg
      781 ggccgacctc ctccgacatg ccccagcgcg cgaccatggc gcgcgccagg ctggtcgcct
      841 ggcggatgtc gtcgtcggcg ccggacgaga cgttgccgag gacctcgcgc tcggcgacgc
      901 gcccgccgag catcaccgcg agcttgtcct tcaggtagtc ctcgggcagc gtgtggcgct
      961 cgacctcggg cagcatgtgg gtgccgccga gcgcgtgccc gcgcgggatg atggtcacct
     1021 tgtacagcgg gtcggcggtg ggcaggtagt gggcgaccgc ggtgtggccg ctctcgtgca
     1081 ccgccaggcg atggcgctcc tcgggctgga tcgcgagcgt gcgcaccgag cccatcagca
     1141 gcttgtcgcg catggcgtcg aactggcgca tggtgaccgc cgcctcgccg tcgcgcgccg
     1201 ccgccatcgc cgcctcgttg accaggttct tgaggtcggc gccggagaag ccgggcgtgc
     1261 cggcggcgat gacatcgagg tcgacgtcgc cgccgagcgg caccttgcgc gtatgcaccc
     1321 tgaggatcgc ggcgcgcgcg cgcttgtcgg gaagctccaa ggtgacgtgc cggtcgaagc
     1381 ggccggggcg cagcagcgcc gggtcgagca cgtcgggccg gttggtcgcg gccagcacga
     1441 tcaccgcctc gtggcccgag aagccgtcca tctcggacag gatctggttg agcgtctgct
     1501 cgcgctcgtc gtggccgccg ccgaggccgg cgccgcgcac ccgcccgacc gcatcgagct
     1561 cgtcgatgaa gatgatgctg ggcgcgcgct tcttcgcctc ctcgaacatc ttgcgcaccc
     1621 ggctggcgcc gacgccgacg aacatctcga tgaactcgga cgccgagatg tggaagaacg
     1681 gcaccccggc ctcgccggcg agagcgcgcg cgagcagggt cttgccggtc ccaggcgggc
     1741 cgaccagaag gacgccgcgc ggcggctcgg cgccaagccg ggtgtagcgg tcgggcgcgc
     1801 gcaggaagtc gaccagctcc gccacctcgc gcttggcgtt gtcctggccg gcgacgtcgt
     1861 cgaagcgcac cttgggcgca cccgccttgg tcgccctggt ggccgagccg gccaggaagt
     1921 cgccgatgtc gcgcccgccg aaccggccgg caagattgct gtaggtgcgg ttccagaacc
     1981 accagtagac cgcgatcagc atcagccagg gcagcaggcc gaccagccag gtgccgaccc
     2041 cgtccgcttt cggcaggctc cggtattcga cattgtgctg ctcgagcagc ggcaggaggg
     2101 cgtcgtcgcc gacctcgggc acgcgcgtgg caatgcgcga gaccgcggcg ccctgggggt
     2161 cggtgacgcc ggcgccggag accagctcgg cggcgatgtc gccgtcgcgg aagctggccc
     2221 gcaccacctt gtcggcggcg accagctgct tgaaggcgct gtagggcagc tccaggctcg
     2281 gcgccggccg gtcgatgccg gtgccgagcg cgatccagat cgccagcaag ccggcgacca
     2341 gcgcgaacgg cagccatttg ggcgtccggt ccaggacctt cctcctccag cggttgcggc
     2401 ccgaccacca taccgcgtcg gggccgcgat gtcaggccgc gcgcagctca gtgcgccatc
     2461 agcaccggca cggtcatgtg ctcgaggatg tggcgggtgg cgccgccgaa caccgcctcg
     2521 cgcagccgcg agcggccgta ggcgcccatc acgatcaggt ccgagcccag gtcgctggcc
     2581 atgttgagca gctcgtcgcc gacctcgatg tcgggggcga cgctgtggcg cgcctcgacg
     2641 tgcacgccgt gatgggcgag gtgggcggcg atatcggccc ccgggatgtg gccctgctcg
     2701 ggcgggtcga cggccagcac caccaccgac tcggccgccc gcaacagcgg caatgcatcg
     2761 tgcaccgcgc gcgtcgcctc gcggctcgcg ttccacgcca ccagcgcgcg ccggccgagg
     2821 tcgtcgtggc ggccggcata ggggatggtc agcagcggcc ggccgctggc cagcgccagc
     2881 tcgcccggga agccctgcgg gcgcgcgccg tcctgctcgg ggtccggctg gccgatgatg
     2941 atcaggtcgg catagcggcc gtgctcgacc agcaggtcgc gggcggcgcc gggcgcctcg
     3001 cgccattcga aatgcacgtc ggcgcggccg gcggcggcct cgagccgggc gcgggcgcgc
     3061 gcggcatggg cgagataggc ctcgcgcgcg cgcctgagcg cctcctcgcc gatctgcgcc
     3121 gcgacgtagc cgggaagctc ggcgaactcg agcgcgaaca gggcgatcag gcgggcgtcg
     3181 tggcggcggg cgagcgcgca ggcggcggcg aagcgagccg cgcccgccgg ctgttcctcg
     3241 gcgtgaacga gtatgtcctt gtacaccggt cgaatcctca cagggcaggg tcgcggcgcg
     3301 cagggcgcgg ggcttgacgg cgacggtgcg gcggcgcgcg ggccgccgcc ttagcctgga
     3361 tcaaggcccg tgcacctggc gcggccggcg gcgccggtca cggcgcgtgc gcgccggcac
     3421 cgacaaccgc ccgcagccgg gcgcggccgc ctattggtcg ccctggtagg gcgagtagcg
     3481 cttcaggtag gcgatgatct tggccctggg ctcgtcctcg accttggcga acatggtggt
     3541 gccgcggatg aagtcctggg tgctggtcag ccagccgtcg agcgtatcct cgtcccagcg
     3601 ccacgggttc tcgagcgcgg cgcgaaacgc gcgcgagtag gggtagtttt ccatgatgcc
     3661 cgcgggccgg tcgtagacgc cggtcaagga cgggccgagc ggcatggtca ccgccaggtt
     3721 gagcagggcc tcgggcgtcc agctgaccgc gtcctcgttg accagtccgt ggcatttcga
     3781 gcaggtgttg atgtacggct cgcggccgtc ctcggcgtcg tcggcccagg ccgcgactgc
     3841 tcccagcatg acggccagcg tcgcgagtat cggcgcgacc aggcgccgcg gtgctgaggt
     3901 cattgagtcc attcgggcgt ctccatctgt cggtctcgat cactgggctg gcgtcgggat
     3961 tcgccggcac gtaccggcgc cggacggcgg ccaccttagc acagatcaag tccggtcgcg
     4021 gtcgcgcacc ttcgggccgg cgccgggcgc accgccggac gatgcgcaag gtggcgcccg
     4081 cggggtcaac cgacaaaccc aagtcatgct gctatcccac ggaaatcttg accatttggc
     4141 agccgccgag gtccccgcat tgcgccttgc aatcgcgtcg cggtgcgtat taaatacgcg
     4201 cggtttggtg ccacactaag gctcgggcgg cgcatcgaaa catgggccga ggttcgactt
     4261 ggcgagggtc cgcgcgcgtc tccgcgatcg ggggcgaggg gtccgcgacc ggcgagcgag
     4321 tccgggacac ggtcgggaca taccgggacg ggtcgtcgct gcggatcgcg gtcgatgagc
     4381 ggcggatcgc cggatctgac agcccggcga taggtgtccg cggcgcacgc cgcccggtcg
     4441 aacgcaagct cggcgtcacc tatgcccgca tgccaggcct ggccgacgag ccggaagcgc
     4501 cggtggctgt cgatggcggt tgcgcctggc ggacccaggc gaaggccgtc atctgcggcg
     4561 ggctcgttta cgtaggtgcg gtcggagccg ccggcaacgt gttcgcgtcc ggcggcacgc
     4621 ttggggccgc ggtcgaggca gccgttgtcg ccggcggcgt cggcgcactg atcggcggcg
     4681 cggtctcgat gggactcgac cggcgtcccg gcggatcggc gggtcggcgg atcgcccgcg
     4741 gcggtaaaag ccgtttcgtc tgacgtcttg cgtgcagctt tcaggggcca ggagcaacgg
     4801 ggaatagcgg atgcatgtcg cactcatact gatcctggtg gccgttggct cgctcgcgtt
     4861 tcacttgctg agcccgtggt ggtgga
//
LOCUS       DASWHU010000397         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1787408, whole genome shotgun sequence.
ACCESSION   DASWHU010000397 DASWHU010000000
VERSION     DASWHU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787408"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..988
                     /gene="ispH"
                     /locus_tag="VGB88_14665"
                     /gene_synonym="lytB"
     CDS             56..988
                     /gene="ispH"
                     /locus_tag="VGB88_14665"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9648745.1"
                     /translation="MRVILAQPRGFCAGVERAIEIVESAINRYGKPVYVRHEIVHNKR
                     VVEELRAKGAVFVEELDEVPENGVTVFSAHGVSQKVTDDARRRELPVIDATCPLVAKV
                     HKEAQRYAAQGRQVILIGHKGHPEVEGTSGRVPGGVLLVSSPGEVAQLEVRDPNMLAY
                     VTQTTLSVDDTREVIDALKVRFPTIAGPSLRDICYATQNRQTAVRRLASEVDVILVVG
                     AQNSSNSNRLREIGEETDVPSYLIDDARAIDPAWIANAHTIGITAGASAPEQLVQEVI
                     DRLREMDEVEVEVLDGVAETVQFKLPAELSAEGS"
     gene            998..2155
                     /gene="hpnH"
                     /locus_tag="VGB88_14670"
     CDS             998..2155
                     /gene="hpnH"
                     /locus_tag="VGB88_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX9648746.1"
                     /translation="MAVPLEQAVRVGAYVLAQKLKGRRRYPLVLMLEPLFRCNLACAG
                     CGKIDYPDEILNRRLSVAECLAAVDECGAPVVSIPGGEPLLHKEIDRIVAAIVARRKF
                     VYLCTNALLLKKKLHLFRPSRYLTFSVHLDGLKDDHDRSVCQDGVFERAVEAIRLARD
                     AGFRVNVNCTVFDGHDPERLAAFFDFARDLGVEGVTISPGYAYERAPDQQHFLSRERT
                     KRLFRNVLARGKGRRWRFSQSVMFLDFLAGNQSFGCTPWGNPTRNVFGWQRPCYLLGE
                     GYAASFAELMDGTDWDSYGTGAYQKCANCMVHCGYEPTAVNATVARPLTALGLWLTGV
                     RTDGPMAAEIELSGARPAEYVFDANVSRFLAGADVGEGEAADRSGRREHAA"
     gene            complement(2078..2833)
                     /locus_tag="VGB88_14675"
     CDS             complement(2078..2833)
                     /locus_tag="VGB88_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648747.1"
                     /translation="MGHLSAPATPRAVGIVTGLKAEARLVRRAIARQDLDVDIAVACA
                     GPGPECALRAARMLIEDGAAALASVGLAGGLKPTAWPGMVIVARSVVAPDGTRYDTDA
                     AWRTRLVAALIGDDDVVEGGLCGADAPVLAAADKAALHAATGAHAVDTESHAVAAAAA
                     VAGIPFAAVRAVADGAGRSVPDGLARSVRADGAIAPLVALGWAAARPLRVGSLVGCAL
                     DARRARDSLRRVLAAAGAVGGLAFADIGTGEKA"
     gene            complement(2821..4758)
                     /gene="shc"
                     /locus_tag="VGB88_14680"
     CDS             complement(2821..4758)
                     /gene="shc"
                     /locus_tag="VGB88_14680"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX9648748.1"
                     /translation="MRGRALGAARAPAAAELDRVIDEASAALAAGQRADGHWCFELEA
                     DCTIPAEHVMLGHYLDEIDAALEAKIGRYLRARQGADGGWPLFYGGEADISCSVKAYL
                     ALKLIGDDPGDAHMRRAREAILARGGAARANVFTRIALALFGQVPWRAVPTMPAEIML
                     LPRWFPFHMSKVSYWSRTVIAPLLVLTALKPRARNPRNIDVRELFTTPPEHERHYFAR
                     RGAVAAAFLGLDRVLRAAEPLMPGGVRARAIERAMSFTTERLNGEDGLGGIFPAMANA
                     LMAFDALGYARDHPQRATCRRAIDRLLVVGAEEAYCQPCVSPVWDTALAAHALLEAGG
                     EDARARAAAACAWLEDRQILDGPADWRQSRPGLRAGGWAFQYRNDYYPDVDDTAVAMM
                     ALHRLAPERFAAAIARAGEWIEGVQGTGGGWGAFDVDNTYAYLDHIPFADHGALRDPP
                     TEDVTARCIGALCQAGLGNRSDAVARALDFLRRTQQPDGSWFGRWGTNYVYGTWSVLC
                     ALNAAGIGADDPMVRRAVAWLEARQRSDGGWGEDGASYWPDRRDQVKASTASQTAWAL
                     LALMAAGEADSDAVRRGVDFLLAAPRAGARWHEDWYTAVGFPRVFYLRYHGYAAYFPL
                     WALARYRMLTRGNAQRPAWGI"
     gene            complement(4775..>5467)
                     /locus_tag="VGB88_14685"
     CDS             complement(4775..>5467)
                     /locus_tag="VGB88_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9648749.1"
                     /translation="RLPVRFAIDRAGLVGADGQTHAGSFDVTYLGCLPGFVVMAAADE
                     AELVHMVATAAAIDDRPSAFRYPRGEGVGVEMPERGRPLEIGKGRVVREGGVVAILSL
                     GARLQECLAAADELAARGLSTTVADARFAKPMDEDLVRRLAGEHAVLITIEEGAIGGF
                     AAQVMHFLAHAGMFDRGLKFRPMVLPDRFIDHGTPRGQYQEAGLCAPDIVACALAALG
                     REEAIAAASESA"
BASE COUNT          779 a         2014 c         1977 g          697 t
ORIGIN      
        1 cgatttggct aaggtcgcgc ccgcgaattg cgagcgaacg ggacgtggtg gacggatgcg
       61 ggtgatcctg gcccaaccgc gcggcttctg cgccggcgtc gaacgggcga tcgagatcgt
      121 cgaaagtgcc atcaaccgct atggcaagcc ggtctatgtg cgccacgaga tcgtccacaa
      181 caagcgggtg gtcgaggagc tgcgcgccaa gggcgcggtg ttcgtcgagg agctcgacga
      241 ggtgcccgag aacggggtga cggtgttcag cgcccacggc gtgtcgcaga aggtcacgga
      301 cgacgcgcgc cggcgcgagc tgccggtgat cgacgccacc tgcccgctgg tcgccaaggt
      361 ccacaaggaa gcgcagcgct acgcggcgca gggacgccag gtgatcctga tcggccacaa
      421 gggccatccc gaggtcgagg gcaccagcgg ccgcgtgccc ggcggcgtgc tgctggtctc
      481 gagcccgggc gaggtcgccc agctcgaggt gcgcgatccc aacatgctcg cctacgtcac
      541 ccagacgacg ctcagcgtcg atgacacccg cgaggtgatc gacgccctca aggtgcgctt
      601 cccgaccatc gccgggccgt cgctgcgcga catctgctac gccacccaga accgccagac
      661 cgcggtccgc cggctggcca gcgaggtcga cgtgatcctg gtggtgggcg cgcagaacag
      721 ctcgaactcc aaccgcctgc gcgagatcgg cgaggagacc gacgtgccga gctacctgat
      781 cgacgacgcg cgcgccatcg accccgcctg gatcgcgaac gcgcacacca tcggcatcac
      841 cgccggcgcc tcggctcccg agcagctggt ccaggaggtg atcgaccgcc tgcgcgagat
      901 ggacgaggtc gaggtcgagg tgctcgacgg cgtggccgag acggtccagt tcaagctgcc
      961 ggccgagctg tcggcggaag ggtcgtaggg gcggccggtg gcggttcccc tcgagcaggc
     1021 ggtgcgggtc ggcgcctatg tgctcgccca gaagctcaag ggccggcggc gctatccgct
     1081 ggtgctgatg ctcgagccgc tgttccgctg caacctcgcc tgcgccggct gcggcaagat
     1141 cgactacccg gacgagatcc tgaaccgaag gctcagcgtc gccgagtgcc tggccgcggt
     1201 cgacgagtgc ggcgcgccgg tggtctcgat cccgggcggc gagccgctgc tgcacaagga
     1261 gatcgaccgg atcgtcgccg ccatcgtggc gcggcggaag ttcgtctatc tgtgcaccaa
     1321 cgcgctgctg ctgaagaaga agctgcacct attccggccg agccgctacc tcaccttttc
     1381 ggtgcatctc gacgggctga aggacgacca cgaccgttcg gtgtgccagg acggggtgtt
     1441 cgagcgcgcg gtcgaggcga tccgcctcgc gcgcgatgcc ggcttccggg tcaacgtcaa
     1501 ctgcaccgtg ttcgacggcc acgaccccga gcggctggcc gcgttcttcg atttcgcccg
     1561 cgacctcggc gtcgagggcg tcaccatctc gcccggctac gcctacgagc gcgcccccga
     1621 ccagcagcac ttcctgtcgc gcgagcgcac caagcggctg ttccgcaacg tgctcgcgcg
     1681 cggcaagggg cggcgctggc ggttcagcca gtcggtgatg ttcctcgact tcctggccgg
     1741 caaccagagc ttcggctgca cgccgtgggg caacccgacc cgcaacgtgt tcggctggca
     1801 gcggccgtgc tatctgctgg gcgagggcta tgcggcgagc ttcgccgagc tgatggacgg
     1861 caccgactgg gactcctacg gcaccggcgc ctaccagaag tgcgccaact gcatggtcca
     1921 ttgcggctac gagccgaccg cggtcaacgc gaccgtggcc cggccgctga cggcgctcgg
     1981 cctgtggctc acgggcgtgc gcaccgacgg cccgatggcg gccgagatcg agctgtccgg
     2041 ggcgcgcccg gccgagtacg tgttcgacgc caacgtgtca cgctttctcg ccggtgccga
     2101 tgtcggcgaa ggcgaggccg ccgaccgctc cggccgccgc gagcacgcgg cgtaacgagt
     2161 cccgcgcgcg ccgggcatcg agcgcgcagc cgaccagcga tccaactctc agcggccgtg
     2221 ccgcggccca gccgagcgcc accagcggcg cgatcgcgcc gtccgcgcgc acgctccgcg
     2281 ccaggccgtc cgggaccgag cgcccggcgc cgtcggcgac cgcgcgcact gccgcgaacg
     2341 ggatgcccgc aaccgccgcc gccgccgcaa cggcatggct ttcggtgtcc accgcgtgcg
     2401 cgccggtggc cgcatgcagc gccgccttgt cggcggccgc aagcaccggc gcatcggcgc
     2461 cgcacagccc gccctcgacc acgtcgtcgt cgccgatcag cgccgcgacc aggcgcgtcc
     2521 gccaggcggc atcggtgtcg tagcgggtgc cgtcgggcgc caccaccgag cgggcgacga
     2581 tgaccatgcc gggccaggcg gtgggcttga gcccgccggc gaggccgacg ctcgccagcg
     2641 ccgcggcgcc gtcctcgatc agcatgcgcg cggcgcggag cgcgcactcg ggcccgggcc
     2701 cggcgcaggc gacagcgata tcgacatcga ggtcttgcct agcgatggcg cgccgcacca
     2761 gccgcgcctc ggccttcagg ccggtgacga tgccgaccgc ccgcggcgtc gcgggcgcgc
     2821 tcagatgccc cacgccggcc gttgggcgtt gcctcgtgtc aacatgcggt agcgtgccag
     2881 agcccacagc gggaaatagg cggcatagcc gtgatagcgg aggtagaaga cgcgcgggaa
     2941 tccgaccgcg gtgtaccagt cctcgtgcca gcgcgcgccc gcgcgcggcg ccgcgagcag
     3001 gaagtcgacg ccgcggcgga cggcgtccga gtcggcctct ccggcggcca tcagggcgag
     3061 cagcgcccag gcggtctgcg acgcggtgct cgccttgacc tggtcgcgcc ggtcgggcca
     3121 gtagctggcg ccgtcctcgc cccagccgcc gtcgctgcgc tggcgcgcct cgagccaggc
     3181 gaccgcgcgc cgcaccatgg ggtcgtccgc gccgatgccg gcggcattca gcgcgcacag
     3241 caccgaccag gtgccataga catagttggt gccccaacgg ccgaaccagg agccgtccgg
     3301 ctgctgggtg cgcctgagga agtcgagcgc gcgcgcgacc gcgtccgacc ggttgccgag
     3361 accggcctgg cacagcgcgc cgatgcagcg ggcggtgacg tcctcggtcg gcgggtcgcg
     3421 cagcgcgccg tggtcggcga acgggatgtg gtcgaggtag gcataggtgt tgtcgacgtc
     3481 gaaggcgccc cagccgccgc ccgtgccctg cacgccctcg atccactcgc cggcgcgggc
     3541 gatggccgcg gcgaagcgct ccggcgccag gcggtgcagc gccatcatcg ccaccgcggt
     3601 atcgtcgaca tcgggatagt agtcgttgcg gtattggaac gcccagccgc cggccctcag
     3661 ccccgggcgg gactggcgcc agtcggccgg gccgtcgagg atctgccggt cctcgagcca
     3721 ggcgcaggcg gcggcggcac gcgcccgcgc gtcctcgccg cccgcctcga gcagggcgtg
     3781 cgcggcgagc gccgtgtccc acaccggcga cacgcacggc tggcagtagg cctcctcggc
     3841 gccgaccacc agcagcctgt cgatcgcgcg ccggcaggtc gcccgctgcg gatggtcgcg
     3901 cgcatagccg agcgcatcga acgccatcag cgcgttcgcc atcgccggga agatgccgcc
     3961 gagcccgtcc tcgccgttga gacgctcggt ggtgaagctc atcgcgcgct cgatcgcgcg
     4021 tgcgcgcacg ccgcccggca tcagcggctc ggcggcgcgc agcacccggt cgaggccgag
     4081 gaacgccgcc gccaccgcgc cgcggcgggc gaagtaatgc cgctcgtgct cgggcggggt
     4141 ggtgaacagc tcgcgcacgt cgatgttacg cggattgcgc gcccgcggct tgagcgccgt
     4201 cagcaccagc agcggcgcga tcacggtccg cgaccagtac gacaccttgc tcatgtggaa
     4261 cgggaaccag cgcggcagca gcatgatctc ggccggcatg gtcggcaccg cgcgccacgg
     4321 cacctggccg aacaaagcga gcgcgatgcg cgtgaacacg ttcgctcgcg ccgccccgcc
     4381 gcgcgccagg atcgcctcgc gcgcgcgccg catatgggcg tccccggggt cgtcgccgat
     4441 cagcttgagc gccaggtacg ccttcaccga gcacgagatg tccgcctcgc cgccgtagaa
     4501 cagcggccag ccgccgtcgg cgccctggcg ggcgcgcaga tagcgcccga tcttggcctc
     4561 gagcgcggcg tcgatctcgt cgaggtaatg gccgagcatg acgtgctcgg ccgggatggt
     4621 gcagtcggcc tcgagctcga agcaccaatg gccgtcggcg cgctggccgg cggccagcgc
     4681 cgccgaggcc tcgtcgatga cgcggtcgag ttccgccgcc gcaggcgcgc gcgcggcccc
     4741 tagcgcccgc ccccgcatcg cacggcgtgc ccgctcacgc ggactcggac gccgccgcga
     4801 tcgcctcctc gcggccgagc gccgccagcg cgcaggcgac gatgtcgggc gcgcacagcc
     4861 cggcctcctg gtactggccg cgcggcgtgc cgtggtcgat gaaccggtcg ggcagcacca
     4921 tcgggcggaa cttgaggccg cggtcgaaca tcccggcatg ggcgaggaaa tgcatcacct
     4981 gggcggcgaa gccgccgatc gcgccttcct cgatggtgat cagcaccgcg tgctcgccgg
     5041 cgagccggcg caccaggtcc tcgtccatcg gcttggcgaa gcgcgcgtcg gcgaccgtcg
     5101 tcgacaggcc gcgcgccgcc agctcgtcgg cggcggcgag gcactcctgg aggcgcgcgc
     5161 cgagcgacag gatcgcgacc acgccgccct cgcgcaccac gcggcccttg ccgatctcga
     5221 gcggccggcc gcgctcgggc atctcgacgc cgacgccctc gccgcgcgga tagcggaacg
     5281 ccgacgggcg atcgtcgatc gctgccgcgg ttgccaccat gtgcaccagc tccgcctcgt
     5341 cggcggccgc catcaccacg aagccgggca ggcagcccag ataggtgaca tcgaacgatc
     5401 cggcatgggt ctggccgtcg gcgccgacca ggccggcgcg gtcgatggcg aagcgcaccg
     5461 gcaggcg
//
LOCUS       DASWHU010000398         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_289915, whole genome shotgun sequence.
ACCESSION   DASWHU010000398 DASWHU010000000
VERSION     DASWHU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289915"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VGB88_14690"
     CDS             complement(<1..165)
                     /locus_tag="VGB88_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX9648750.1"
                     /translation="MSRLTLYPAIDLKDGACVRLVQGDMARATVFSTDPPAQARAFAA
                     IGFAWLHLVDL"
     gene            complement(162..680)
                     /locus_tag="VGB88_14695"
     CDS             complement(162..680)
                     /locus_tag="VGB88_14695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9648751.1"
                     /translation="MADLAVQQVERFRRGELAGLCDATVEAILDGIGFGWVQPPPRER
                     LEAYWRGVLLVPERELFLGLLGASVAGSVQLVKPVRNFEAGQFAAHIDTHFVTPWARG
                     HGLARMLLEAAEAEARAQGFTVMRLDVRATQERAIALYESCGYERWGTLDRYHKVDGR
                     MIAGHFYVKDLG"
     gene            complement(680..1321)
                     /gene="hisH"
                     /locus_tag="VGB88_14700"
     CDS             complement(680..1321)
                     /gene="hisH"
                     /locus_tag="VGB88_14700"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9648752.1"
                     /translation="MKVAIVDYGSGNLRSAAKAFERAGAEAGLELRIAVTEDAAEVAG
                     ADRIVLPGVGAFGDCAHGLARLPGMHDALTRAVIEQARPFLGVCVGMQLLAEQGFEHG
                     RHDGLGWIPGAVVALTPRDASLKIPHMGWNELAIARAHPLLDGVGDGAHCYFVHGYQF
                     AVADHADLIACTDHGGPVTAVIGRDNIVGTQFHPEKSQKVGLKVIGNFLRWRP"
     gene            complement(1318..1737)
                     /locus_tag="VGB88_14705"
     CDS             complement(1318..1737)
                     /locus_tag="VGB88_14705"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEX9648753.1"
                     /translation="MRLYTYYMKPGETAPEDAVAVREGFSWAAFLLTFLWAFYHRLWV
                     VGLVLLAVPALVQYAIDSHWLHPAAGSALILAFTIFVGCTANDWRREKMERQGWVLSG
                     IAAGGDLVEADRRFFDKLAHAVPPPSAARLWRTGFRA"
     gene            complement(1792..2397)
                     /gene="hisB"
                     /locus_tag="VGB88_14710"
     CDS             complement(1792..2397)
                     /gene="hisB"
                     /locus_tag="VGB88_14710"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX9648754.1"
                     /translation="MRQATVERNTAETRVRAKVDLDGTGSYRIATGIGFLDHMLQQLS
                     RHSLVDLELEAEGDLHIDFHHTTEDTGIVIGQAVAKALGARRGILRYGSALIPMDETL
                     TRVALDLSNRPYLVWKVAMPRPKLGDFDTELFKEWFHAFAQAAGATLHVETLYGENTH
                     HIVESCYKGLARSLRTAVAVDPRIADLVPSTKGVLGGEGDN"
BASE COUNT          396 a          922 c          936 g          366 t
ORIGIN      
        1 gaggtcgacc aggtgcagcc aggcgaagcc gatcgcggcg aaggcgcgcg cttgcgccgg
       61 cgggtcggtc gagaacacgg tcgcgcgcgc catgtcgccc tggaccaggc gcacgcaggc
      121 gccgtccttg aggtcgatcg ccggatagag cgtgagccgg ctcatccaag gtccttgacg
      181 tagaagtggc cggcgatcat gcgcccgtcg accttgtggt agcggtcgag ggtgccccag
      241 cgctcgtagc cgcagctctc gtagagcgcg atcgcgcgct cctgggtggc gcgcacgtcg
      301 aggcgcatga cggtgaagcc ctgcgcgcgc gcctcggcct cggccgcctc gagcagcatg
      361 cgcgcgagcc cgtggccgcg cgcccagggg gtgacgaaat gggtgtcgat gtgggcggcg
      421 aactggccgg cctcgaagtt gcgcaccggc ttgaccagct gcaccgagcc cgcgaccgag
      481 gcgccgagca ggccgaggaa cagctcgcgc tcgggcacca gcagcacgcc ccgccagtac
      541 gcctccaggc gctcgcgcgg cggcggctgc acccagccga agccgatgcc gtcgaggatc
      601 gcctcgacgg tcgcgtcgca cagcccggcg agctcgccgc gccggaagcg ctcgacctgc
      661 tggaccgcga ggtccgccat cagggccgcc agcgaaggaa gttgccgatc accttgagac
      721 cgaccttctg gctcttctcg gggtggaact gtgtgccgac gatgttgtcg cgcccgatca
      781 ccgcggtcac cggcccgccg tggtcggtgc aggcgatcag gtcggcgtgg tccgcgaccg
      841 caaactgata gccatgcacg aaatagcagt gggcgccgtc accgacgccg tcgagcagcg
      901 ggtgcgcgcg cgcgatcgcg agctcgttcc agcccatgtg cgggatcttg agcgaggcat
      961 cgcgcggcgt gagcgcgacc accgcgccgg ggatccagcc gagcccgtca tgacggccgt
     1021 gctcgaagcc ctgctcggcc aacagctgca tgccgacgca gacgccgagg aacgggcgcg
     1081 cctgttcgat caccgcacgc gtgagcgcat cgtgcatgcc gggaaggcgc gcgaggccgt
     1141 gggcgcagtc gccgaacgcg ccgacgccgg gcagcacgat gcggtcggcg cccgcgacct
     1201 cggccgcgtc ctcggtcacc gcgatgcgga gctcgagccc ggcctcggcg ccggcgcgct
     1261 cgaacgcctt ggccgccgag cgcaggttgc ccgagccgta gtcgacgatc gcgactttca
     1321 tgcccggaag cccgtgcgcc acaggcgcgc cgccgacggc ggcggcacgg cgtgcgcaag
     1381 cttgtcgaag aacctccggt cggcctcgac caggtcgccg ccagcggcga tgcccgacag
     1441 cacccagccc tggcgctcca tcttctcgcg ccgccaatca ttggccgtgc aaccgacgaa
     1501 gatggtgaag gcgaggatca gcgccgagcc ggccgccggg tgcagccagt gcgaatcgat
     1561 cgcgtactgg accagcgccg gcaccgccag cagcaccagc ccgaccaccc aaagccggtg
     1621 gtagaacgcc cacaggaagg tgagcaggaa cgccgcccac gagaagccct cgcgcaccgc
     1681 gaccgcgtcc tcgggcgccg tctcgcccgg cttcatgtaa taggtgtaga gccgcatcgg
     1741 gcttcctgtt cccggacctc gcgcgccgcc acgggccggg cgcgccgcca gtcagttgtc
     1801 gccttctccg cccagcacgc ccttggtcga gggcaccagg tctgcgatcc gcgggtcgac
     1861 cgcgaccgcc gtgcgcaggc tgcgggcgag gcccttgtag caggactcga cgatgtggtg
     1921 ggtgttctcg ccgtacaggg tctcgacgtg gagggtggcg cccgcggcct gggcgaaggc
     1981 gtgaaaccac tccttgaaca gctcggtgtc gaagtcgccg agcttgggcc gcggcatggc
     2041 gaccttccac accaggtagg ggcggttgga caaatcgagc gcgacccggg tcaaggtctc
     2101 gtccatcggg atcagcgcac tcccgtagcg caggatgccg cggcgcgcgc cgagcgcctt
     2161 ggcgaccgcc tggccgatga cgatgccggt gtcctcggtg gtatggtgga agtcgatgtg
     2221 gagatccccc tcggcctcga gctcgaggtc gaccaggctg tggcgcgaca gctgctggag
     2281 catgtggtcg aggaagccga tgccggtggc gatccggtat gagccggtgc cgtcgagatc
     2341 gaccttcgcc cggacccggg tctcagcggt gttgcgctcg accgttgcct ggcgcatggc
     2401 ggggagccct cgcatgcctg gatgcgccgc ccttttatca gcatcatggc gggcgagaaa
     2461 ggcccctcgg cgcgcctttc ggccccaccc cttgaccggg gcggcgatat cgccacgtta
     2521 ggggctgcct ttcaccggaa ccgtcccgat cctggccatc tcatgacatc tcggaccaag
     2581 gccggctggc gcggcaccac cgtgctgtcg gtgcgcaagg
//
LOCUS       DASWHU010000399         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1041452, whole genome shotgun sequence.
ACCESSION   DASWHU010000399 DASWHU010000000
VERSION     DASWHU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041452"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB88_14715"
     CDS             complement(<1..266)
                     /locus_tag="VGB88_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648755.1"
                     /translation="MDQTSAAGYPIAAPPLGGRNVHGPTAAVGAGALADSPPFWGEDG
                     FTFDDLLDLINPLQHIPLLGSLYRAWTGDEIAFGPRVLGGALFAG"
     gene            complement(385..1518)
                     /gene="ribA"
                     /locus_tag="VGB88_14720"
     CDS             complement(385..1518)
                     /gene="ribA"
                     /locus_tag="VGB88_14720"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX9648756.1"
                     /translation="MDQLSFEPIEAEAPAAVIAVDRAIGDLRRGGVVVLADAAGGASL
                     VLSCEYAGARRLARFDALARAPAALVLTARRAAALGITAAASGVAALQLAAPPDPALV
                     HDLSDPAAARAAGTALPAPAAASARDGAAVELAKLASLLPAVVTKSLDRTAAASLAAW
                     AAARGLLVVRAADIDRHRRLAPAALERISAARVPLADAEDTTIVAFRPVDGGAEHLAM
                     VIASPDPKAAVLARIHSQCFTGDLLASLRCDCGDQLRGAIRAIAEAGGGVVLYLAQEG
                     RGIGLVNKLRAYQLQDQGRDTFQANEELGFDADERHWRVAAEMLRQLGFARVRLLTNN
                     PEKIAGLRRAGIEVAERVAHAVPANPHNRRYLAAKAARGGHLL"
     gene            1668..1952
                     /locus_tag="VGB88_14725"
     CDS             1668..1952
                     /locus_tag="VGB88_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9648757.1"
                     /translation="MAKATWNGGVVAESDRVELVEGNVYFPPEAVVRAHLEPSGTTTW
                     CHWKGVAHYYHVVVDGKRNPDAAWYYPEPMDAARQIRDHVAFWHGVTVAR"
     gene            2002..2700
                     /locus_tag="VGB88_14730"
     CDS             2002..2700
                     /locus_tag="VGB88_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9648758.1"
                     /translation="MEPSGTQKRILLVEDDETLRQSLSEQLQLHEEFETVEADSGASA
                     LSAAKGQRFDLVLLDIGLPDMDGREVCRLMRRGGIKTPIVMLTGADTEADTILGLDSG
                     ANDYITKPFRLGVLLARIRAQLRHHELTDDATFGIGPYSFQPAAKLLIDNTSRKKVRL
                     TEKETAILKYLYRAGDKVVGREVLLDEVWGYNAAVATHTLETHIYRLRQKIEADPSNA
                     QILVTETGGYRLNR"
     gene            complement(2690..>3913)
                     /locus_tag="VGB88_14735"
     CDS             complement(2690..>3913)
                     /locus_tag="VGB88_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9648759.1"
                     /translation="AAPPPHGTRWSGVGFGIALGALAAYAHFKLPPALPAMLAEFGYG
                     RLLAGALMSVFAVAGLALSAPIGRALSRLGIGRFLAAALALLAAGGGAGLIWPEGGAV
                     MLAARALEGVAYAILGVAGAVIVAESASPRQRPIALSLYAGWMPYGQVAAVLVAAASA
                     GLDPWRGLWLAGIAAAAGLGLWGAWLSRRGLLPQPAAPVGARHGAPARASPARRRAIL
                     AGALLYTLWTTQWMAFMTWLPQFLVEAHGLDLRAAVLGYAVPPAVVIGFNLVGGAALR
                     RGTPLGLLLLPGLALQAAVWLSMAVVGSGLAGVLALAAYGLGTGLTAVCLFALPGALA
                     GPGLDIARGFGVLMTGRNIGVLIGPMLLAEVLERAGDWAAVGPLFAAVALAALALAAW
                     LARVLSRLAAPAVSG"
BASE COUNT          576 a         1453 c         1485 g          399 t
ORIGIN      
        1 ccggcgaaca gcgcgccgcc gagcacgcgc gggccgaacg cgatctcgtc gccggtccag
       61 gcgcgataga gcgagccgag gagcgggatg tgctggagcg ggttgatcag gtccaagagg
      121 tcgtcgaagg tgaagccgtc ctcgccccag aagggcggcg aatcggcgag ggcgccggcg
      181 cccacggcgg ccgtcgggcc gtggacgttg cgcccgccga gcggcggggc ggcaatcggg
      241 tatccggctg cgctggtctg gtccatggcc gtgctcgggt gcggaaatgc aagccgcggg
      301 ccaggctcgc ggccgcggaa tgccgggctt cgacccgcgc gggcaatgtc cggcccggca
      361 ggatttgccg cttgtgcgga ccgtctagag caggtggccg ccgcgcgcgg ccttggccgc
      421 caggtagcgc cggttgtgcg ggttggccgg caccgcgtgg gcgacgcgct cggcgacctc
      481 gatgccggcg cgccgcaggc cggcgatctt ctcggggttg ttggtcagca ggcgcacgcg
      541 ggcgaagccg agctggcgca gcatctcggc ggcgacccgc cagtggcgct cgtcggcgtc
      601 gaagccaagc tcctcgttgg cctggaaggt gtcgcgcccc tggtcctgga gctggtaggc
      661 gcgcagcttg ttgaccaggc cgatgccgcg gccctcctgg gccaggtaca ggaccacccc
      721 gccgccggcc tcggcgatgg cgcggatggc gccgcgcagc tggtcgccgc agtcgcagcg
      781 caggctcgcc agcaaatcgc cggtgaagca ctgcgagtgg atgcgcgcga gcaccgccgc
      841 cttcgggtcg ggcgacgcga tcaccatcgc caggtgctcg gcgccgccgt cgaccgggcg
      901 gaaggcgacg atggtggtgt cctcggcgtc ggcgaggggg acgcgcgcgg cgctgatccg
      961 ctcgagcgcg gcgggcgcga gccggcggtg gcggtcgatg tcggccgcgc gcaccaccaa
     1021 caggccgcgc gccgccgccc aggcggcgag gctggcggcc gccgtccggt ccaggctctt
     1081 ggtcaccacc gccggcagca ggctggcgag cttggcgagc tcgaccgcgg ccccgtcgcg
     1141 cgccgaggcc gcggctggcg ccggcagcgc ggtgccggca gcgcgcgccg cggcgggatc
     1201 ggagaggtcg tggaccagcg ccggatcggg cggcgcggcg agctggagag ccgccacacc
     1261 gctcgcggcc gcggtaatgc cgagcgcggc ggcgcgccgg gcggtgagca cgagcgccgc
     1321 cggcgcgcgc gccagggcgt cgaagcgggc gaggcggcgc gccccggcat attcgcacga
     1381 cagcaccagc gaggcgccgc cggccgcatc cgccagcacc accacgccgc cgcggcgcaa
     1441 atcgccgatc gcccggtcga cggcgatcac cgccgccggc gcctcggcct cgatcggctc
     1501 gaacgacagc tggtccatca cgcgaccctt gcgccgccgg gggcgcggcg gcggcgcccg
     1561 ttatataggc gcgcggccgg tgcccgccag cgctagcttg ccccggcccg ggcgcgctaa
     1621 actcgccgcc agggttcggc ccggcgaggg gccagggaga cgggaccatg gccaaggcaa
     1681 cctggaacgg cggggtggtc gccgaaagcg accgcgtcga gctggtcgag ggcaacgtct
     1741 acttcccgcc cgaggcggtg gtgcgcgccc acctcgagcc cagcggcacc accacctggt
     1801 gccactggaa gggtgtcgcg cactactacc acgtggtggt cgacggcaag cgcaacccgg
     1861 acgccgcgtg gtactatcca gagccgatgg acgcggcgcg ccagatccgc gaccacgtcg
     1921 cgttctggca cggcgtcacg gtcgcacgct agccgcatcc agggggcggg cgccgccagg
     1981 gcgcccgata cgtgacaggc aatggaaccg tcgggaacgc aaaagcgcat cctgctggtc
     2041 gaggacgacg agacgctgcg ccaatcgctg tccgagcaat tgcagctgca cgaggagttc
     2101 gagaccgtcg aggccgattc gggcgcctcc gccctgagcg ccgccaaggg ccagcgcttc
     2161 gacctggtgc tgctcgacat cgggctgccc gacatggacg ggcgcgaggt ctgccggctg
     2221 atgcggcgcg gcggcatcaa gaccccgatc gtcatgctca ccggcgccga caccgaggcc
     2281 gataccatcc tcggcctcga ttcgggcgcc aacgactaca tcaccaagcc gttccggctc
     2341 ggcgtgctgc tggcgcgcat ccgcgcccag ctgcgccacc acgagctcac cgacgacgcc
     2401 accttcggca tcggcccgta cagcttccag cccgcggcca agctgctgat cgacaacacc
     2461 agccgcaaga aggtccgcct gaccgagaag gagaccgcga tcctcaaata cctgtatcgc
     2521 gccggcgaca aggtggtcgg gcgcgaggtg ctgctggacg aggtgtgggg ctacaacgcg
     2581 gcggtcgcca cccataccct cgagacccac atctaccggc tgcgccagaa gatcgaggcc
     2641 gacccgtcga acgcgcagat cctggtcacc gagaccggcg gctaccggct caaccgctga
     2701 cggcgggcgc ggccaggcgg ctgaggacgc gcgcgagcca ggcggcgagc gccagcgcgg
     2761 cgagcgcgac ggcggcgaac agcggcccga ccgccgccca gtcgccggcc cgctccagca
     2821 cctcggcgag caacatcggc ccgatcagca cgccgatgtt gcgcccggtc atcagcacgc
     2881 cgaagccgcg ggcgatgtcg agccccggcc cggccagcgc gcccggcagc gcgaacaggc
     2941 agaccgcggt gagcccggtg ccgaggccgt aggcggcgag cgccagcacc ccggcgaggc
     3001 cgctgccgac caccgccatc gacagccaca ccgccgcctg cagcgcgagc cccggcagca
     3061 gcagcaggcc gagcggcgtg ccgcggcgca gtgccgcgcc gccgaccagg ttgaacccga
     3121 tcaccaccgc cggcggcacc gcgtagccga gcacggcggc gcgcaggtcg aggccgtgcg
     3181 cctcgaccag gaactgcggc agccaggtca tgaacgccat ccactgggtg gtccagagcg
     3241 tgtacagcag ggcgccggcg aggatcgccc ggcgccgcgc cggggacgcg cgcgcgggcg
     3301 cgccgtgccg cgcgccgact ggcgcggccg gctggggcag caggccgcgc cgggaaagcc
     3361 aggcgcccca caggccgagg ccggcggccg ctgcgatgcc cgcgagccac agcccgcgcc
     3421 aggggtctaa tcccgcgctc gcggcggcaa ccaggactgc cgcgacctgg ccgtagggca
     3481 tccagccggc gtacagcgac agcgcgatcg ggcgctggcg gggggatgcg ctctcggcca
     3541 cgatcaccgc cccggcgacg ccgaggatcg cgtaggcgac gccttcgagc gcgcgcgcgg
     3601 ccagcatcac ggcgccgcct tcgggccaga tcaggccggc gccgccgccg gccgccagca
     3661 gcgccagcgc cgcggcgagg aagcgcccga tgccgaggcg gctcaaggcc cgcccgatcg
     3721 gcgccgacag cgccagcccc gcgaccgcga acaccgacat cagcgcgccg gcgagcaggc
     3781 ggccgtagcc gaactcggcg agcattgccg gcagcgccgg cggcagcttg aaatgggcgt
     3841 aggccgcgag cgcgccgagc gcgatgccga acccgacccc ggaccagcgg gtgccgtgcg
     3901 gcggcggggc ggc
//
LOCUS       DASWHU010000400         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1792687, whole genome shotgun sequence.
ACCESSION   DASWHU010000400 DASWHU010000000
VERSION     DASWHU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792687"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGB88_14740"
     CDS             complement(<1..292)
                     /locus_tag="VGB88_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648760.1"
                     /translation="MAEPLIRARDLARDYRAGGAIVQALAGVTLDIARGEFVAVMGPS
                     GSGKSTLMHLLGLLDRPSSGGYWFEGRDVGRLGGDERAAVRNRKVGFVFQSFN"
     gene            complement(294..1640)
                     /locus_tag="VGB88_14745"
     CDS             complement(294..1640)
                     /locus_tag="VGB88_14745"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9648761.1"
                     /translation="MIRALIVIVLLGLVGAAVVGLRSRAAGGEVSYVTAPVERGDLIT
                     TVTATGTLKAVVTVEVSSQLSGQVAEVHADFNDEVAKDQPIARLDQSILAAKVREAEA
                     ALEVARAQVLIEQAAAEKSEADLANARAARAVVDERTTGLAARLEEAKRDLERKEALA
                     EKGTMSQSEVDRARTEHLAAASALGAEKAQRLVEDAAILSAEAAVRMARAEVVNAGAV
                     VTQKQAALEQAEIELARTVIRSPIDGVVVGRDVDRGQTVAASLEAPKLFEIAQDLRRM
                     ELHAKVDEADIGRIRVGPTATFTVDSFPDRSFEGTVVQIRKAPEVVENVVTYTVVISA
                     ENPDLLLLPGMTAIVRVVVDETRDVLKLPNAALRFRPEGGETGGDVGEPDDASSAVAW
                     VKGADGRPVAVALRIGATDGTATEQVEGPLGEGDEVIVGRTVIREEPALFGLRFGF"
     gene            1972..2400
                     /locus_tag="VGB88_14750"
     CDS             1972..2400
                     /locus_tag="VGB88_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9648762.1"
                     /translation="MRIAEVSRQAGVGVETVRFYESRGLIRRPPRPATGGYREYSEDT
                     VRRIRFVRRAQRLGFSLSGIIELLELETVPGARCIDVRERAIAKLADVETKLERLGRI
                     KGALEELVRACPGQGPAKRCSILAAIKSGDLHLEATGKEE"
     gene            2402..2674
                     /locus_tag="VGB88_14755"
     CDS             2402..2674
                     /locus_tag="VGB88_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9648763.1"
                     /translation="MANKRRKIEIFSAGCAICEDVVETVRQAACPSCDVRVLDLRATE
                     VQRRATELGVRSVPAVAIDGRLAECCTTRGVDVEVLRAAGLGQPVA"
     gene            2688..3038
                     /locus_tag="VGB88_14760"
     CDS             2688..3038
                     /locus_tag="VGB88_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HEX9648764.1"
                     /translation="MKAHLIAAGGVVSALAASACCVGPLLLVSLGVSGAWIGSLTALA
                     PYQPVFLGGAILCLGLGFWLVYGRARRVCPVDAGAGARRFLVGFLGVKGALWLGTALV
                     TLAAAVDFGLPLFL"
     gene            3047..3325
                     /locus_tag="VGB88_14765"
     CDS             3047..3325
                     /locus_tag="VGB88_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9648765.1"
                     /translation="MKARVLGAMLLALAAPAALAGERTVTLSVERMTCELCPITVAKA
                     IERVAGVVDVAVDYQAGTAVVTFDDDVTTWSEVAAASTNAGYPAHPIQ"
     gene            3334..3537
                     /locus_tag="VGB88_14770"
     CDS             3334..3537
                     /locus_tag="VGB88_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HEX9648766.1"
                     /translation="MELDSTITCPHCAHAATERMPTDACQYFYQCTHCGVMLRPKHGD
                     CCVFCSYGTTPCPPMQAAATGAG"
     gene            complement(3594..>4901)
                     /locus_tag="VGB88_14775"
     CDS             complement(3594..>4901)
                     /locus_tag="VGB88_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9648767.1"
                     /translation="VEGARIAALGAAAAVAVPAGARIIDGRGLWLIPGLIDAHVHLAN
                     SAGLYSSPSNLDLRAIRDYQTDELARVRAALPATLLRYLASGVTAVVDRGGPAWSLEI
                     KALADTVPAPRVALSGPMLATYRQAAFLGELAHGRWIRTPEEARAAVRANVRAGVDLI
                     KIHFLPRVGGVTSDLAWARAAIDEAHAQGLKVCVHATQLALARALVEAGADQLVHSID
                     DAPVDDALVALLAERGTVYVTTLLVYEGYREVFLRGLDYSDIERRLGDPQVIETLDDI
                     AWIDDRHLPPWLKADHPARGARTASAAAWPHWAVSDVRAIQDQNLRRLAAAGVTIAAG
                     TDAGTIGNLHGPALHRELELMAEAGLAPLQVLKAATLGAAAALGRSADLGSIAPGKLA
                     DMVLLEADPLADIRNTRQIRAVIKDGVHYAAADLEAALAAMAP"
BASE COUNT          662 a         1707 c         1808 g          724 t
ORIGIN      
        1 agttgaacga ctggaacacg aagccgacct tgcggttgcg gacggcggcg cgctcgtcgc
       61 cgccgagccg gcccacgtcg cggccctcga accagtagcc gcctgagctc ggccggtcga
      121 gaagcccgag caggtgcatc agggtcgact tgccggagcc cgacggcccc atgaccgcga
      181 cgaactcgcc gcgggcgatg tcgagcgtca ccccagccag cgcctgcacg atcgcgccgc
      241 cggcgcggta atcgcgggcg aggtcgcgcg cccggatcag cggctcggcc acctcagaac
      301 ccgaaccgga ggccgaacag ggccggctcc tcgcggatca cggtgcggcc gacgatgacc
      361 tcgtcgccct cgccgagcgg cccctcgacc tgctcggtag cggtgccgtc ggtcgcgccg
      421 atgcggagcg cgaccgccac cggcctgccg tcggcgccct tgacccacgc caccgccgag
      481 ctggcgtcgt cgggctcgcc gacatcgccg cccgtctctc cgccctcggg acggaagcgc
      541 agcgccgcgt tgggaagctt gagcacgtcc ctggtctcgt cgacgacgac gcgcacgatc
      601 gcggtcatgc cgggcagcag caggaggtcc gggttctcgg ccgagatcac caccgtgtag
      661 gtgaccacgt tctccaccac ctcgggcgcc ttgcgaatct ggacgacggt gccctcgaag
      721 ctgcggtcgg ggaacgagtc gaccgtgaac gtggcggtgg gcccgacccg gatgcggccg
      781 atgtcggcct cgtcgacctt ggcgtgaagc tccatgcggc gcaggtcctg ggcgatctcg
      841 aacagcttcg gcgcctccag gctggccgcc acggtctggc cgcggtcgac gtcgcgcccg
      901 accaccacgc cgtcgatcgg cgagcggatg acggtgcggg cgagctcgat ctcggcctgc
      961 tcgagcgccg cctgcttctg cgttaccacg gcgccggcgt tgacgacctc ggcgcgggcc
     1021 atgcgcacgg ccgcctcggc gctcaggatc gcggcgtcct ccacgagcct ttgcgccttc
     1081 tcggcgccga gcgccgacgc cgccgccaag tgctcggtcc gcgcgcggtc gacctcgctc
     1141 tgcgacatcg tgcccttctc ggccagcgcc tccttgcgct cgaggtcgcg cttggcctcc
     1201 tccaggcgcg ccgccagccc ggtcgtgcgc tcgtcgacga ccgcgcgcgc ggcgcgcgcg
     1261 ttggcgaggt cggcctcgga cttctcggcc gcggcctgct cgatcagcac ctgggcccgg
     1321 gcgacctcga gcgccgcctc ggcctcgcgc accttggcgg ccaggatcga ctggtcgagc
     1381 cgggcgatgg gctggtcctt ggcgacctcg tcgttgaaat cggcgtggac ctccgcgacc
     1441 tggccggaaa gctgcgagct cacctcgacg gtgaccaccg ccttgagcgt gccggtggcg
     1501 gtgaccgtgg tgatcaggtc gccgcgctcg accggcgcgg tcacgtagct cacctcgccg
     1561 ccggccgcgc gcgagcgcag gccgacgacc gccgccccga ccaggccgag cagcacgatg
     1621 acgatgagcg cgcggatcac gggcctgtgg tcctcctcgg gcggcagcgg gcctcagtta
     1681 caaatgtggg gccgcggcgg ggttcggcca tgccggcggc cacggacgtc gggccgacac
     1741 gcagcgacgg ctcggtgatc catccggatt tcgagcccac gagcctcggg gagcatcgct
     1801 gcaagctcgg cggtgtcgta gaccacaccg cctggatctc ctcacggggc gcgaggccag
     1861 ctgccgcgat cagatcgagc tcgaagaaac gcggagggga cgagcgcgat cgaggcttga
     1921 ctccgtagta tggtacggcc ggtagcatcg aggctcgatc gaggaggacg catgaggatt
     1981 gccgaggttt caaggcaggc gggggtcggc gtcgagacgg tccggttcta cgagagccgg
     2041 ggcctgatcc ggcggccgcc gcgcccggcg accggcggct acagggagta ttccgaagac
     2101 acggttcggc gcatccggtt cgttcgccgc gcccagcggc tcggcttctc gctcagcggg
     2161 atcatcgagc tcctggagct cgaaaccgtg ccgggcgccc gctgcatcga cgtgcgcgag
     2221 cgcgcgatcg ccaagctcgc cgacgtcgaa acgaagttgg aacggctggg ccggatcaag
     2281 ggcgcgctcg aagagctggt tcgcgcctgc cccggccaag gcccggccaa gaggtgctcg
     2341 atcctggcgg cgatcaagtc cggagacctg cacctcgagg cgacaggcaa ggaggaatga
     2401 gatggcgaac aagcggcgca agatcgagat tttcagcgcg ggctgcgcga tctgcgagga
     2461 tgtcgtcgag acggtccggc aggcggcgtg cccgtcgtgc gacgtccggg tcctcgacct
     2521 gagggcgacc gaagtgcagc ggcgggcgac cgagctcggc gtcagatcgg tcccggcggt
     2581 cgcgatcgac ggccggcttg ccgagtgttg cacgacccgt ggcgtcgacg tcgaggtgct
     2641 tcgcgccgcg ggacttgggc agccggtggc atagcggcgg gcggcgcatg aaggcgcatc
     2701 ttatcgccgc gggcggcgtt gtttccgcgt tggcggcgtc ggcgtgctgc gtcggacccc
     2761 tgctgctcgt ctccctcggc gtgagcggcg cctggatcgg cagcctgacc gcattggcgc
     2821 cctaccagcc ggtcttcctt ggcggcgcga tcctgtgtct cggcctcggg ttctggctgg
     2881 tctatgggcg cgcccggcgg gtctgcccgg tcgatgccgg cgcgggcgcg aggcgattcc
     2941 tcgtgggctt tctgggggtc aagggcgcgc tgtggcttgg aactgcactg gtcacgctcg
     3001 cggcagcggt cgatttcggc ctgcccctgt tcttgtgagg acgaaaatga aggctcgtgt
     3061 gcttggtgcg atgctgctgg cgctggcagc cccggcggcg cttgccggcg agcggacggt
     3121 gaccctgtcg gtcgagcgaa tgacctgcga gctgtgtccg atcaccgtcg ccaaggcgat
     3181 cgaaagggtt gccggggtgg ttgatgtcgc ggtcgattac caggccggca ccgcggtcgt
     3241 gacgttcgac gacgacgtga cgacatggtc ggaggtcgcc gcggcctcga ccaacgccgg
     3301 ttatccggcg catccaatcc aatagcgcgc tcaatggaac tcgactccac catcacctgt
     3361 ccgcattgcg ctcacgccgc gaccgagcgg atgcccaccg acgcgtgtca gtatttctac
     3421 caatgcacgc actgcggcgt gatgctgcgg cccaagcacg gcgactgctg cgtgttctgc
     3481 tcttacggca ccacgccgtg cccgccgatg caggccgcag cgaccggcgc gggttaggcc
     3541 gatgtgccga ggctctattc aggcaagtcg gccggtttgc gagtcggcac gtctcaaggc
     3601 gccatagccg cgagcgcggc ttcgaggtcg gcggcggcat agtgcacgcc gtccttgatg
     3661 acggcgcgga tttggcgggt gttgcggatg tcggcgagcg ggtcggcctc gagcagcacc
     3721 atgtcggcga gcttgccggg ggcgatcgag ccgaggtcgg cgctgcggcc cagcgcggcg
     3781 gcggcgccca atgtcgcggc cttgagcacc tggaggggcg cgaggcccgc ctcggccatc
     3841 agctcgagct cgcggtgcag cgccgggccg tgcaggttgc cgatggtgcc ggcgtcggtg
     3901 ccggcggcga tggtgacgcc ggcggcggcg aggcggcgca ggttctggtc ttggatcgcg
     3961 cgcacgtcgc tgaccgccca gtgcggccac gccgcggcgc tggccgtgcg cgccccgcgc
     4021 gccgggtggt cggccttgag ccaaggcggc aggtggcggt cgtcgatcca ggcgatgtcg
     4081 tcgagggtct cgatcacctg cgggtcgccc agccggcgct cgatgtcgct gtagtcgagg
     4141 ccgcgcagga acacctcgcg gtagccctcg tagaccagca gcgtggtgac gtagaccgtg
     4201 ccgcgctcgg ccaagagcgc gaccaaagcg tcgtcgaccg gcgcgtcgtc gatgctgtgc
     4261 acgagctggt cggcgccggc ctcgaccaac gcccgggcga gcgcgagctg agtggcatgg
     4321 acgcacacct tgagcccctg cgcgtgagcc tcgtcgatcg ccgcgcgcgc ccaggcgagg
     4381 tcgctcgtga ccccgccgac ccgcggcagg aaatggatct tgatcaggtc gaccccggcg
     4441 cgcacgttgg cgcgcaccgc cgcgcgcgcc tcctccggcg tcctgatcca gcggccatgg
     4501 gcaagctcgc cgaggaaggc ggcctgccgg taggtggcga gcatcggccc ggacagggcg
     4561 acgcgcggcg cgggcacggt gtcggcgagc gcctttatct ccagcgacca tgccggcccg
     4621 ccgcggtcga ccacggccgt caccccgctc gccaggtagc gcagcaaggt cgcgggcagc
     4681 gccgcgcgca cccgcgccag ctcgtcggtc tggtagtcgc ggatcgcgcg cagatcgaga
     4741 ttggacgggc tcgagtacag gcccgccgag ttggcgagat ggacgtgggc gtcgatcagc
     4801 cccgggatca gccacagccc gcgcccgtcg atgatgcgcg cgccggccgg cacggcgacc
     4861 gcggccgcgg cgccgagcgc ggcgatgcgc gcgccctcga c
//
LOCUS       DASWHU010000401         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3257773, whole genome shotgun sequence.
ACCESSION   DASWHU010000401 DASWHU010000000
VERSION     DASWHU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257773"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1117
                     /locus_tag="VGB88_14780"
     CDS             221..1117
                     /locus_tag="VGB88_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648768.1"
                     /translation="MLDPTLILVDGVVYASWLFLVAVGLTLIYGVMNILNVAHGSLYA
                     LGAFTAAWAVGEYLGGGGSEAATYVLLLLSAIAVGLVFGVVIERGVLRWMYGRDEIVI
                     VLVTYAVFLILEDLIKLVFGVQSYMPYQPRNLLGYVNVGAIPFAAYDFCLIALAVVVG
                     LAVWFGVNRTRPGKLLHAVIYDREISRALGIDVTRVFTVTFVIGAMLGALGGAATAPM
                     ISVVPGIGVEIIVLAFAVVVIGGLGSIEGAAIGALLVGLGRTLAVNMLPEVELFIVYG
                     VMALVLVIRPQGLFGRAEARKI"
     gene            1120..2064
                     /locus_tag="VGB88_14785"
     CDS             1120..2064
                     /locus_tag="VGB88_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9648769.1"
                     /translation="MRRDLTPAALTIALAVLLAAGLVLPGWAVNTVALSLANGLVVLG
                     LMIQMRAGLVSFGQALYYGLGAYAAALADTFLGVTDFAALLVIGVAAAVAVAFVLGFL
                     LSRYRGIFFAMLSLALSMILFGLLVNSAALGSTDGFGVSPPTYFGTVPAGDSRLSFQL
                     YGLVCLLAYGAAWAAHRYLNSPMGYIGEAIRENEVRVEYLGASVRRAIHLKYVLAAAV
                     SGLGGVITALLIGHVTPEDFVYWTRSGEFVFVAILSGTGSVAAPFIGSMLFELVRTYA
                     LEYAPDFWQMLLGATLLVVILFLPGGLWSVVRLATGRR"
     gene            2066..2827
                     /locus_tag="VGB88_14790"
     CDS             2066..2827
                     /locus_tag="VGB88_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648770.1"
                     /translation="MAALLAAEGLNKRFGAVVAAEQVSLSVEAGETVGVIGANGAGKT
                     TFVNMVTGYLKPDSGAIRHDGRDITGLKPRRIARLGIHRSFQIPQIFPELAVFDNMLI
                     AHGIARETRPGAWRALLDADIAARAEQDLSRFGILDYRDQVAGKLPQGVRKLLDIAMA
                     MVGDTGLMLLDEPTSGISADQKTGVMDTIAAALEAAGVTMLFVEHDMEVVERYAPRVV
                     AFYDGRVIADGPTGTVLADADVRTYVIGPELHRRR"
     gene            2832..3497
                     /locus_tag="VGB88_14795"
     CDS             2832..3497
                     /locus_tag="VGB88_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648771.1"
                     /translation="MLSVRALTVQIAAATILDDVDLEVETGTLTALMGRNGAGKTTFM
                     RSVMGLIPVRAGSVDFDGTDIARLAAHRRAGLGIGYMPEDRRLVPELTVRENVLVPAW
                     AARIADAEARLARIYRLMPEVESFAGRRAMQLSGGQQKLVALARALMSGTRMVLLDEP
                     FEGLAPALARRLVEVLSGLRDQGLTVLLSESDSTHSEDLVDAVYVIERGRIGARTGAV
                     GAG"
     gene            3499..>4079
                     /locus_tag="VGB88_14800"
     CDS             3499..>4079
                     /locus_tag="VGB88_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9648772.1"
                     /translation="MKRVAIVGAGPAGLYAAYLLKRARPDIALEVVEQNPADATFGFG
                     VVFSDSALAFLDDEDPDTHDMIAARTERWHDIAVVHRGERIVIDGVGFAAIGRLALLR
                     LLQARARSVGVEPRFGTAVSSLDELGEADLIIGADGVNSVVRRSDPVAFGQRIAHLAN
                     HFIWYGTRKPFATLTQSFRETADGAFNAHHYRYA"
BASE COUNT          526 a         1399 c         1443 g          711 t
ORIGIN      
        1 gcagttaggc aagcaaacaa gcggccggca agcgcagcgc gcctgtcggc ccgaccgtga
       61 gcgtgtcaat gaaccggctg tcgtccttcg agacgctcgc ttcgctcgct cctcaggacg
      121 aggacctgtc gtgactcaat cattgcctca ccctgaggag cgcccaaagg gcgcgtctcg
      181 aagggggcgg caagaacgcc ccaggctctg acaccgcccc atgctcgatc cgaccctgat
      241 cctggtcgac ggcgtcgtct acgcctcgtg gctgttcctg gtcgcggtcg ggctgacgct
      301 gatctacggc gtcatgaaca tcctcaacgt cgcccacggc agcctgtatg cgctcggcgc
      361 gtttaccgcc gcgtgggcgg tcggtgagta tctcggcggc ggcgggtccg aggcggcgac
      421 ctatgtgctg ctgctgctgt ccgcgatcgc ggtcgggctg gtgttcggcg tggtcatcga
      481 gcgcggcgtg ctcaggtgga tgtacggccg cgacgagatc gtgatcgtgc tggtcaccta
      541 cgccgtgttc ctgatcctcg aggacctgat caagctggta ttcggggtcc agtcctacat
      601 gccctaccag ccgcgcaacc tgctcggcta tgtcaacgtc ggcgcgatcc cgttcgcggc
      661 ctacgacttc tgcctgatcg cgcttgcggt ggtggtcggg ctcgcggtct ggttcggtgt
      721 caaccgtacg cgcccgggca agctgctgca cgcggtgatc tacgaccgcg agatcagccg
      781 cgcgctcggc atcgacgtga cgcgggtgtt cacggtcacc ttcgtcatcg gcgccatgct
      841 cggcgcgctc ggcggcgccg cgaccgcgcc gatgatctcg gtggtgcccg ggatcggcgt
      901 cgagatcatc gtgctcgcct tcgcggtggt ggtgatcggc gggctgggca gcatcgaggg
      961 ggcggcgatc ggcgcgctgc tggtcgggct cgggcgcacg ctcgcggtca acatgctgcc
     1021 cgaggtcgag ctgttcatcg tctacggggt catggcgctg gtgctggtga tccgcccgca
     1081 gggcctgttc gggcgcgcgg aagcgcgcaa gatctgacca tgcgccgcga cctgacgccg
     1141 gcggcactca ccatcgcgct cgcggtcctg ctcgccgccg ggctggtgct gcccggctgg
     1201 gcggtcaaca cggtcgcgct gtcgctcgcc aacggcctgg tggtgctcgg cctgatgatc
     1261 cagatgcgcg ccggcctcgt gtccttcggc caggcgctct attacgggct cggcgcttat
     1321 gccgcggccc tcgccgacac cttcctcggc gtcaccgact ttgccgccct gctggtgatc
     1381 ggggtcgcgg cggcggtggc ggtggcgttc gtgctcggct tcctgttgtc gcgctaccgc
     1441 ggcatcttct tcgccatgct cagcctggcg ctgtcgatga tcctgttcgg cctcctggtc
     1501 aacagcgccg cgctcggcag caccgacggc ttcggcgtct cgccgccgac ctatttcggc
     1561 accgtgcccg cgggcgactc gcggctgagc ttccagctct acggcctggt ctgcttgctc
     1621 gcctatggcg cggcgtgggc cgcgcatcgc tacctgaatt cgccgatggg ctatatcggc
     1681 gaggcgatcc gcgagaacga ggtccgggtc gagtatctgg gcgcttcggt gcggcgcgcg
     1741 atccacctca aatacgtgct cgcggccgcg gtctcagggc taggcggggt gatcaccgcg
     1801 ctcctgatcg gccacgtcac gcccgaggac ttcgtctact ggaccaggtc gggcgagttc
     1861 gtgttcgtcg ccatcctgag cggcaccggc agcgtcgcgg cgccgttcat cggctcgatg
     1921 ctgttcgagc tggtgcgcac ctacgcgctc gaatacgcgc ccgacttctg gcagatgctg
     1981 ctcggcgcga cgctgttggt ggtgatcctg ttcctgcccg gcgggctgtg gtcggtggtc
     2041 cggctggcca ccggcaggcg ctgagatggc ggcgctcctg gcggccgagg ggctgaataa
     2101 gcggttcggc gcggtggtcg cggccgagca ggtgtcgctg tcggtcgagg ccggcgagac
     2161 ggtcggcgtg atcggcgcca acggcgccgg caagaccacc ttcgtcaaca tggtcaccgg
     2221 ctatctcaag cccgactcgg gcgcgatccg ccacgacggg cgcgacatca ccgggctcaa
     2281 gccgcgccgg atcgcccgtc tcggcatcca ccgctcgttc cagattccgc agatctttcc
     2341 cgagctcgcc gtgttcgaca acatgctcat cgcgcacggc atcgcgcgcg agacgcggcc
     2401 gggggcgtgg cgtgccttgc tcgacgccga catcgccgcc cgcgccgagc aggacctgtc
     2461 gcgcttcggt attctcgatt accgcgacca ggtcgccggc aagctgccgc agggtgtgcg
     2521 caagctgttg gacatcgcca tggcgatggt cggcgatacc ggcctgatgc tgctcgacga
     2581 gccgaccagc ggcatcagcg ccgaccagaa gaccggggtg atggacacca tcgccgcggc
     2641 gctggaggcg gccggcgtga cgatgctgtt cgtcgagcac gacatggagg tggtcgagcg
     2701 ctacgcgccc cgggtggtgg cgttctacga cggccgggtc attgccgacg ggccgaccgg
     2761 aacggtgctc gcggacgcgg acgtgcgcac ctacgtgatc ggccccgagc tgcaccggcg
     2821 acggtgagcc gatgctgagt gtccgcgcgc tcaccgtcca gatcgccgcg gcgacgatcc
     2881 tcgatgacgt cgacctcgag gtcgagaccg gcacgctcac cgcgctgatg ggccgcaacg
     2941 gcgccggcaa gaccaccttc atgcgcagcg tcatgggact gatcccggtg cgcgccggca
     3001 gcgtcgactt cgacggcacc gacatcgccc gcctggccgc gcaccggcgc gcggggctcg
     3061 gcatcggcta catgcccgag gaccgccgcc tggtgcctga gctcaccgtg cgcgagaacg
     3121 tgctggtgcc ggcctgggcg gcacggatcg ccgacgccga ggcgcggctc gcgcgcatct
     3181 accggctgat gcccgaggtc gagtccttcg ccggccggcg cgcgatgcag ctttcgggcg
     3241 gccagcagaa gctggtcgcg ctggcgcgcg cgctgatgtc gggcacccgc atggtgctgc
     3301 tcgacgagcc gttcgagggc ttggcgccgg cgctcgctcg gcgcctggtc gaggtgctgt
     3361 ccggcctcag ggaccagggg ctgacggtgc tgctgtcgga atcggattcg acccactccg
     3421 aggacctggt cgatgcggtc tacgtgatcg agcgcgggcg catcggcgcg cgcaccggcg
     3481 ccgtcggcgc gggatgacat gaagcgtgtc gcgatcgtcg gcgccgggcc ggcgggcctg
     3541 tacgcggcct atcttctcaa gcgggcgcgc cccgacatcg cgcttgaggt ggtcgagcag
     3601 aacccggccg acgcgacatt cggcttcggg gtcgtgttct cggacagcgc gctcgccttt
     3661 ctcgacgacg aggaccccga tacccacgat atgatcgccg cgcgcaccga gcgctggcac
     3721 gacatcgcgg tcgtccaccg gggcgagcgc atcgtcatcg acggcgtcgg cttcgccgcg
     3781 atcggccggc tcgcgctcct gcgcctgttg caggcgaggg cgcggtcggt cggcgtcgag
     3841 cctcgcttcg gcaccgcggt ctcgtccctc gacgagctcg gcgaggcgga cctgatcatc
     3901 ggcgcggacg gggtgaactc cgtggtcagg cgctcggacc cggtcgcgtt cgggcaacgg
     3961 atcgcgcact tggctaacca tttcatctgg tacgggacgc gcaagccgtt cgcgacgctc
     4021 acccagtcgt tccgcgagac tgccgacggg gcgttcaacg cccatcatta ccgctacgc
//
LOCUS       DASWHU010000402         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_293385, whole genome shotgun sequence.
ACCESSION   DASWHU010000402 DASWHU010000000
VERSION     DASWHU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293385"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGB88_14805"
     CDS             complement(<1..463)
                     /locus_tag="VGB88_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9648773.1"
                     /translation="MSELGTASILALRLIATLDAELVEIVARSLAVSLSAVVLAAAVG
                     LPLGAAVAVYRFPGRRALVVLLNALMGLPPVFVGLMLYVLLSRAGPLGFLGLLFTPTA
                     MVIAQAVLVTPIIAALSHRVLRDLYEGYREQLCSLGARPWDAVGTLLWDARV"
     gene            complement(531..1376)
                     /locus_tag="VGB88_14810"
     CDS             complement(531..1376)
                     /locus_tag="VGB88_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9648774.1"
                     /translation="MAGTDHSRRRRGAGGAGPAVVLVEPQLGANIGAAARAMLNFGLA
                     DLRLVRPRDRWPSPEAVANASGADEVLARARLFDTLAEAVGDLSYVLATTARARDMTK
                     DVLTPDAAAAELRAAAVRGEGSGVVFGRESTGLDNDALALADAVIAVPSNPGFSSLNL
                     AMAVLLVGYEWYRAGEGTPAQAVLSGRARAATKAELLGLFEQLEAELDACGFLRVADK
                     RPTMVRNLRNLLQRARLTEQEVRTLRGVITGLVEHGRSTLRSGSGAAAGARDGRVGPG
                     AARRK"
     gene            complement(1383..>1982)
                     /locus_tag="VGB88_14815"
     CDS             complement(1383..>1982)
                     /locus_tag="VGB88_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX9648775.1"
                     /translation="SDQAGRANIVHRFAELGIDIDPDSPGIGRLVKLVKEREAAGFAY
                     DGAAASFELLARRTLGSVPDYFHLTGFRVLDEQRTDARGERVTLSEATMKVVVKGAPV
                     MTVAEGNGPVDALAQGLKKALIGAYPVLADTRLVDYKVRILTPEAGTGAAIRVMIESE
                     DGAGRRWATVGVSTNVIAASYDAMHDAITYRLLRDRLDL"
BASE COUNT          280 a          740 c          726 g          236 t
ORIGIN      
        1 tgacgcgcgc gtcccacaag agcgtgccga ccgcgtccca cgggcgcgcg ccgagcgagc
       61 acagctgctc gcgatagccc tcgtagaggt cgcgcagcac ccggtgcgac agcgcggcga
      121 tgatcggggt gaccagcacc gcctgcgcga tcaccatcgc ggtcggggtg aacagcaggc
      181 cgagaaagcc gagcggcccg gcgcgcgaca gcagcacgta cagcatcagg ccgacgaaca
      241 ccggcggcag ccccatcagg gcattcagca gcaccaccag cgcgcgccgg ccagggaacc
      301 ggtagaccgc gaccgcggcg ccgagcggca ggccgaccgc ggcggcgagc accaccgcgc
      361 tcaggctgac cgcgagcgag cgcgccacga tctcgaccag ctcggcgtcg agcgtggcga
      421 tcagcctgag cgcgaggatc gatgcggtgc cgagctcgga catggcgacg agaccgtctc
      481 ccggccgggg atcataggtg gccggcccga ggccgaccag cgtccgccgg tcacttgcgc
      541 cgggccgcgc ccggaccgac gcgcccgtcc ctcgcaccag cggcagcgcc gctgccgctg
      601 cggagggtgg agcggccgtg ctcgaccagg ccggtgatca cgccgcgcaa ggtgcgcacc
      661 tcctgctcgg tgaggcgcgc gcgctgcaac aggttgcgca ggttgcggac catggtcggg
      721 cgcttgtcgg ccacgcgcag gaagccgcag gcgtcgagct cggcctcgag ctgctcgaac
      781 aggcccaaga gctcggcctt ggtggcggcg cgcgcgcgcc cggacagcac cgcctgcgcc
      841 ggcgtcccct cgccggcccg gtaccattcg tagcccacca ggaggaccgc catggcgagg
      901 ttgagcgacg agaagcccgg gttgctcggc accgcgatca cggcatcggc gagcgccagt
      961 gcgtcgttgt cgaggccggt gctctcgcgc ccgaacacga cgcccgagcc ctcgccgcgc
     1021 acggcggccg cgcgcagctc ggcggcggcg gcgtcggggg tgagcacgtc cttggtcatg
     1081 tcgcgcgcgc gcgcggtggt cgccagcaca taggacaggt cgccgaccgc ctcggcgagg
     1141 gtgtcgaaca gccgggcgcg cgccagcacc tcgtcggcgc cggaggcgtt ggccaccgcc
     1201 tcggggctcg gccagcggtc gcgcgggcgc accaggcgca ggtcggcgag accgaagttg
     1261 agcatcgccc gcgccgcggc gccgatgttg gcgccgagct gcggctcgac caacaccacc
     1321 gccgggccgg caccgcccgc gccgcgccgc ctgcggctgt gatcggtccc ggccattgcc
     1381 cgtcagaggt cgagccggtc gcgcagcagg cggtaggtga tggcgtcgtg catggcgtcg
     1441 tagctcgccg cgatgacgtt ggtcgacacg cccaccgtcg cccagcgccg ccccgccccg
     1501 tcctcgctct cgatcatcac ccggatggcg gcgccggtgc ccgcctcggg cgtgaggatg
     1561 cgcaccttgt agtcgaccaa gcgcgtgtcg gcgagcaccg ggtaggcgcc gatcaacgct
     1621 ttcttgagcc cctgggcgag cgcatcgacc gggccgttgc cctcggccac ggtcatcacc
     1681 ggcgcgccct tgaccaccac cttcatggtc gcttccgaca aggtcacgcg ctcgccgcgc
     1741 gcgtcggtgc gctgctcgtc gagcacccgg aagccggtca ggtggaagta gtcgggcacg
     1801 ctgcccagcg tccgccgcgc caagagctcg aagctcgccg ccgccccgtc ataggcgaag
     1861 cccgcggcct cgcgctcctt gaccagcttg accaggcggc cgatgccggg cgagtcgggg
     1921 tcgatgtcga tgccgagctc ggcgaagcgg tggacgatgt tggcgcgccc ggcctggtcc
     1981 ga
//
LOCUS       DASWHU010000403         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3260796, whole genome shotgun sequence.
ACCESSION   DASWHU010000403 DASWHU010000000
VERSION     DASWHU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3260796"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VGB88_14820"
     CDS             <1..157
                     /locus_tag="VGB88_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEX9648776.1"
                     /translation="DGQTTAHYVAERLAGSGVAVTRLAHGVPVGGELDYLDDGTLTAA
                     LKARQKL"
     gene            complement(166..1269)
                     /gene="rmuC"
                     /locus_tag="VGB88_14825"
     CDS             complement(166..1269)
                     /gene="rmuC"
                     /locus_tag="VGB88_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9648777.1"
                     /translation="MTVGTVIAVLLALGLVLIGAVAVVALVRARGGDGHAAAVVERVS
                     ALSGRLEQMAVGAAAGQAELRRAFDERLDAIAKRVGDTLTEQGRHHHSLLEGLKERLA
                     VIDQAQKNITELSGQVVGLQDVLANKQARGAFGEIQLNDLVSSILPPSAYRFQAPVGE
                     GRRADCLLMLPNPPGAIAVDAKFPLESYHALRTAEDDAARIQARRGFRDAILKHVADI
                     AERYIVPGETAESALMFVPSEAVYAELHANFPEVVERSFKARVWIVSPTTLMATLNTV
                     RAVLKDAHMREQAGVIQDEVLRMLGDVARLAERVDSLEKHFEQANKDVRQIRTSADKI
                     TGRGERITQLELGQAGEQADATLAPPEQRDVAE"
     gene            1355..>1816
                     /gene="def"
                     /locus_tag="VGB88_14830"
     CDS             1355..>1816
                     /gene="def"
                     /locus_tag="VGB88_14830"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9648778.1"
                     /translation="MAILPVIVAPDARLKEVCEPVEAVDDAVRRLMDDMLETMYDAPG
                     VGLAAPQVGVRTRVIVVDVAREGQAPQPLPMANPRLVSASAETAVYEEGCLSLPEQFA
                     EVRRPARCTIAYLDYRGARRTLDAEGLLATCVQHEMDHLDGILFVDRISKIK"
BASE COUNT          254 a          641 c          655 g          266 t
ORIGIN      
        1 cgacggccag accaccgccc attacgtcgc cgagcgcctc gcgggcagcg gggttgcggt
       61 cacccgcctc gcccacggcg tgcccgtggg cggcgagctc gactatctcg acgacggcac
      121 gctcaccgcc gcgctgaaag cgcgccagaa gctgtagaga gggtattact cggcgacgtc
      181 gcgctgctcg gggggcgcca gcgtggcgtc ggcctgctcg ccggcctgcc cgagctcgag
      241 ctgggtgatg cgctcgccgc gccccgtgat cttgtcggcc gaagtgcgga tctggcggac
      301 gtccttgttg gcctgctcga agtgcttttc gagcgagtcg acgcgctcgg cgaggcgcgc
      361 cacgtcgccg agcatgcgca gcacctcgtc ctggatcacc cccgcctgct cgcgcatgtg
      421 cgcgtccttg agcaccgcgc gcacggtgtt cagggtcgcc atcagggtgg tcggggagac
      481 gatccacacc cgcgccttga acgagcgctc gaccacctcg gggaagttgg cgtggagctc
      541 ggcgtagacc gcttccgagg gcacgaacat cagcgccgac tcggcggtct cgccgggcac
      601 gatgtagcgc tcggcgatgt cggcgacgtg cttgaggatg gcgtcgcgga agccgcgccg
      661 cgcctggatg cgggcggcgt cgtcctcggc ggtcctgagc gcgtggtagc tctcgagcgg
      721 gaacttggcg tcgaccgcga tcgcccccgg cgggttgggc aacatcaaca ggcagtcggc
      781 gcgccgcccc tcgcccaccg gcgcctggaa gcggtaagcc gaagggggga ggatgctgct
      841 gaccaggtcg ttgagctgga tctcgccgaa cgcgccgcgc gcctgcttgt tggcgagcac
      901 gtcctgaagg ccgaccacct ggccggacag ctcggtgatg ttcttctgcg cctggtcgat
      961 caccgcgagg cgctccttga gcccctcgag caggctgtgg tggtggcggc cctgctcggt
     1021 gagggtgtcg ccgacgcgct tggcgatggc gtcgaggcgc tcgtcgaacg cgcgcctgag
     1081 ctcggcctgg cccgccgcgg cgccgaccgc catctgctcg agccggcccg acagcgcgct
     1141 cacccgctcg accaccgccg ccgcgtgccc gtcgccgccg cgcgcgcgca ccagcgccac
     1201 caccgcgacc gcgccgatga gcaccaggcc gagcgcaagc aggaccgcga tcaccgtgcc
     1261 gacggtcatg gcgcgaactt agcatggcct tgacgcggcg cggccccgat aataggtcaa
     1321 gggccggttg caaagaggcg aagccaagga aatcatggcg atattgccgg tcatcgtggc
     1381 gccggacgcg cgcctgaaag aggtctgcga gccggtcgag gcggtcgatg acgcggtccg
     1441 ccggctgatg gacgacatgc tcgagaccat gtacgacgcc cccggcgtcg gcctcgccgc
     1501 gccgcaggtg ggggtgcgga cgcgcgtcat cgtggtcgat gtcgcgcgcg agggccaggc
     1561 gccgcagccc ttgccgatgg ccaacccgcg cctggtatcg gcgtccgcgg agaccgcggt
     1621 ctacgaggag ggctgcctgt cgctgcccga gcagttcgcc gaggtgcgcc ggccggcgcg
     1681 ctgcacgatc gcctatctcg attaccgcgg cgcccggcgc acgctggacg ccgagggcct
     1741 gctcgcgacc tgcgtccagc acgagatgga ccacctcgac ggcatcctgt tcgtcgaccg
     1801 catctcgaag atcaag
//
LOCUS       DASWHU010000404         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3604360, whole genome shotgun sequence.
ACCESSION   DASWHU010000404 DASWHU010000000
VERSION     DASWHU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604360"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VGB88_14835"
     CDS             complement(<1..926)
                     /locus_tag="VGB88_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648779.1"
                     /translation="MAKRKVETAATLAAAAVAPPRMLDACPEPEQILYGTAEPPPFPA
                     GNIFPIEWDVETPKLVEIYDQARDPGWAPDRLPWDGLDPDSFTADQRYAIAYWFTLLS
                     VFDSSGPAVFARAMIHTYETHEEDPIRKCFFSVTRDEVNHEEICQRAITTLVPGGPLR
                     HVPTTPLGELALNNARWYYHNGARYWEGFKGAIGKYPLAILFTSFLMGELAAATLFHD
                     MHRRTTIPVFRDAFRCVGKDEARHLAICMAVLRKVLPLADAATKATITKQVRAGYVFL
                     SGILYEPPAQFWELPETFRPAHALLEEAARGAG"
     gene            complement(1026..2657)
                     /gene="ipdC"
                     /locus_tag="VGB88_14840"
     CDS             complement(1026..2657)
                     /gene="ipdC"
                     /locus_tag="VGB88_14840"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HEX9648780.1"
                     /translation="MATSLSRRLLRALSDRGVRELFGIPGDFALPFFAEVEASAILPL
                     YTLSHEPAVGFAADAAARARCTLGAAAVTYGAGALNMVNAVAAAYAEKSPVVVISSAP
                     GRAESRRGLLLHHQGKTIGSQFEIYTHITCAQARLDQPERAPAEIARVLTAARVHALP
                     VYIEIPRDMADAPCAAVPEDVMPVPDPDALAACADDVLARLTEAERPVLMAGVELRRY
                     RLEDKVADLAQRLGVPVVTSFMGRGLLAGSQPRALGTYLGVAGEPRLARLVEGSDALL
                     LLGVIPSDTNFGVSERQIDARASIHAYDGQVKVGYRAYGDISLAALVDALLARVAPGS
                     RAAAAADGPGYPRGLEADGAPVTPRDIACAVNDLMAAHGAMPIASDVGDCLFTAMEMA
                     QTALVAPGYYAGMGFGVPAGIGLQIALGERPLILVGDGAFQMTGWELGNCRRYGLDPI
                     VIVFNNRSWEMLRVFQPDSLFNDLDDWHFADIAGPLGGAGRRVETRAELAAALASAIA
                     ERGRFQLIEVMLAEGAISDTLARYVDAVRRGRMAH"
     gene            complement(2694..3440)
                     /locus_tag="VGB88_14845"
     CDS             complement(2694..3440)
                     /locus_tag="VGB88_14845"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9648781.1"
                     /translation="MLLSDLTLIDRPSPNNDARGADAAIDMLVLHYTGMDSTDAAVER
                     LCDPDAAVSAHYLIDDQGAVYRLVPESRRAWHAGVSSWAGDTDINDRSIGIELANPGH
                     QLGYRPFPDAQMTALEHLALDILSRQPIPTRRVVGHSDIAPARKQDPGEFFDWRRLAA
                     KGIGLWPAPTARLRPRPALCRGSSGVEVAAAQSALAAYGYGLAPSGHYDQATVDVVAA
                     FQRHFRPRTVDGAFDVATEALLKALLAMIL"
     gene            complement(3554..4384)
                     /locus_tag="VGB88_14850"
     CDS             complement(3554..4384)
                     /locus_tag="VGB88_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX9648782.1"
                     /translation="MAGENSINTNVGAMVALRYLNLTNAQLSKVQDRVSTGLRVIGAK
                     DNASSFAIAQGIRGDIKGIAAVQQGLSNGRGVAAVAIAGATAVSNMLIELKAKVIEGL
                     NPGNTSEQQAILDADYAALVTQVLSFIQNAEYNGRNILQSGSTAINVIGDIDGGAITL
                     RAQDLEGTAFSALNAQNIANVTAASAALTQVNVVVNTINVALGQLGADARKLDFQDDF
                     LATIADAVEEGLGGLVDADLAKESAKLQALQVKQQLAVQTLGIANASPQILLGLFGSR
                     "
     gene            complement(4638..>5468)
                     /locus_tag="VGB88_14855"
     CDS             complement(4638..>5468)
                     /locus_tag="VGB88_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX9648783.1"
                     /translation="LAFGPLGDDAMLSPRVAPATIGLGLLEAVPEATILALADPADAD
                     GDGVSGRPNRVAGEVGGGIALGRFGWKANQPSLRRQNAAAALGDIGLTSTLFPDENCA
                     APQDACRAAKSGGAPELSDAFLDKLTLYTRLLAVPRQRGADAEPVRHGEQLFRAAGCA
                     ACHMPTLVTGAHPLAELAGQVIHPFTDLLLHDMGEGLADGRPDFAATGREWRTAPLWG
                     LGLVETVNGHTYFLHDGRARNLAEAILWHGGEAEAARERFRALPRGDREALLAFLRSL
                     "
BASE COUNT          784 a         1886 c         2034 g          764 t
ORIGIN      
        1 ccggcgccgc gcgccgcctc ctcgagcaac gcatgcgcgg ggcggaaggt ctcgggcagc
       61 tcccagaact gcgcgggcgg ctcgtacagg attcccgaca ggaacacgta gccggcgcgc
      121 acctgcttgg tgatggtcgc cttggtcgcg gcgtccgcca gcggcagcac cttgcgcagc
      181 accgccatgc agatcgcaag gtggcgcgcc tcgtccttgc cgacgcagcg gaacgcgtcc
      241 cggaacaccg ggatggtggt acgccggtgc atgtcgtgga acagggtcgc cgccgccagc
      301 tcgcccatca ggaacgaggt gaacaggatc gccagcggat atttgccgat cgcgcccttg
      361 aagccttccc aatagcgcgc gccgttgtgg tagtaccagc gcgcgttgtt gagcgcgagc
      421 tcgcccagcg gcgtggtcgg cacatggcgc agcggccctc ccggcaccag ggtcgtgatc
      481 gcgcgctggc agatctcctc gtggttgacc tcgtcgcggg tgaccgagaa gaagcacttg
      541 cggatcgggt cctcctcgtg ggtctcgtaa gtgtggatca tggcgcgcgc gaacaccgcc
      601 gggcccgagc tgtcgaacac cgagaggagc gtgaaccagt aggcgatcgc gtagcgctgg
      661 tcggcggtga acgagtcggg atcgagcccg tcccagggca gccggtcggg ggcccagccg
      721 ggatcgcgcg cctggtcgta gatctcgacc agcttgggcg tctccacgtc ccactcgatc
      781 gggaagatgt tgccggcggg gaacggcggc ggctcggccg tgccgtagag gatttgctcc
      841 ggctcggggc acgcatccag catgcgcggc ggtgccaccg cggccgcggc gagcgtcgcc
      901 gccgtctcaa ccttgcgctt ggccatcctc acgtcctttc gcgcttgccg cagtctaggt
      961 gcgccgccgc ccggatcaaa gagcaatggc tagccacgga tgcccccggc cgtccttgat
     1021 cggggttaat gcgccatgcg cccgcggcgg acggcgtcga catagcgcgc cagggtatcc
     1081 gagatcgcgc cctccgccag catcacctcg atcaactgga agcgcccgcg ctcggcgatg
     1141 gcggaggcga gcgccgcggc gagctcggcg cgagtctcga cccgccggcc ggcgccgccg
     1201 agcgggcccg cgatgtcggc gaagtgccag tcatcgaggt cgttgaacag ggagtcgggc
     1261 tggaacacgc gcagcatctc ccagctccgg ttgttgaaca cgatcacgat cgggtcgagg
     1321 ccgtagcgcc ggcagttgcc gagctcccag ccggtcatct ggaacgcgcc gtcgccgacc
     1381 aggatcaagg gccgctcgcc gagcgcgatc tgcaggccga tgcccgccgg tacgccaaaa
     1441 cccatgccgg cgtaatagcc cggcgcgacg agggcggtct gcgccatctc catggcggtg
     1501 aacaggcagt cgccgacgtc gctcgcgatc ggcatcgcgc cgtgcgcggc catcaggtcg
     1561 ttgaccgcgc aggcgatgtc gcgcggggtg accggcgcgc cgtcggcctc gaggccgcgc
     1621 gggtagccgg ggccgtcggc cgcggcggcg gcccgcgatc cgggggcgac gcgagcgagc
     1681 agggcgtcga ccaaggccgc gagcgagatg tcgccatagg cgcggtagcc gaccttgacc
     1741 tggccgtcat aggcgtggat cgaggcgcgg gcgtcgatct ggcgctcgga gacgccgaag
     1801 ttggtgtcgg acgggatcac gcccagcagc aggagcgcgt ccgagccctc gaccaggcgc
     1861 gcgaggcgcg gctcgccggc gacgccgaga taggtgccga gcgcccgcgg ctgcgagccc
     1921 gcgagcaggc cgcggcccat gaagctggtg accaccggca cgccgaggcg ctgggcgagg
     1981 tcggcgacct tgtcctcgag ccggtagcgg cgcagctcga ccccggccat cagcaccggc
     2041 cgctcggcct ccgtcaggcg ggcgagcacg tcgtccgcgc aggcggcgag cgcgtccggg
     2101 tcgggcacag gcatcacgtc ctcgggcacc gcagcgcacg gcgcgtccgc catgtcgcgc
     2161 gggatctcga tgtacaccgg caatgcatgg acgcgcgcag cggtcagcac cctggcgatc
     2221 tccgccggcg cccgctcggg ctggtcgagc cgcgcctgcg cgcaggtgat gtgggtgtag
     2281 atctcgaact gcgacccgat cgtcttgccc tgatgatgga gcagcaggcc gcggcgcgat
     2341 tcggcacggc ccggcgcgct cgagatcacc accaccgggc tcttctcggc ataggccgcg
     2401 gcgaccgcgt tgaccatgtt gagcgcgccc gcgccatagg tcacggcggc ggcgcccaag
     2461 gtgcagcggg cgcgtgccgc ggcgtcggcg gcgaagccca cagccggctc gtggctcagg
     2521 gtgtagagcg gcaggatcgc gctcgcctcg acctcggcga agaagggcag ggcgaagtcg
     2581 ccgggaatcc cgaacagctc gcgcacgccg cgatcgctga gcgccctcag caggcgccgg
     2641 gacaggctgg tcgccattca cctacccgcc ggcgcgtgcg gcgacacgcc cacctagaga
     2701 atcatcgcca gcagcgcctt cagcagcgcc tcggtggcga cgtcgaaggc gccgtcgacc
     2761 gtgcgcgggc ggaagtggcg ctggaacgcg gcgaccacgt cgaccgtcgc ctggtcgtaa
     2821 tggccgctcg gcgccaggcc gtagccgtag gccgcaagcg ccgactgggc cgccgccacc
     2881 tcaaccccgc tcgacccgcg gcagagcgcc gggcgcgggc gcagccgcgc cgtcggcgca
     2941 ggccacaggc cgatgccctt cgccgccagc cgccgccagt cgaagaactc gcccgggtcc
     3001 tgcttgcgcg ccggcgcgat gtccgaatgg ccgaccaccc gacgtgtcgg gatcggctgg
     3061 cgcgacagga tgtcgagcgc cagatgctcg agtgcggtca tctgggcgtc ggggaacggc
     3121 cggtagccga gctggtggcc cggattggcc agctcgatgc cgatcgagcg gtcgttgatg
     3181 tcggtgtcgc ccgcccacga gctcaccccg gcatgccagg cgcgccgcga ctcgggcacc
     3241 aggcgataca ccgcgccctg gtcgtcgatc aggtaatgcg cgctgaccgc ggcgtcaggg
     3301 tcgcacaggc gctcgaccgc cgcatcggtc gagtccatgc cggtgtaatg cagcaccagc
     3361 atgtcgatcg ccgcgtccgc gccgcgggcg tcgttgttgg gcgagggacg gtcgatgaga
     3421 gtgagatcgg agagcagcat ccgcacggcc accttcggca acaatataag ggtgggcgcg
     3481 gccgccgctc acaaaaatct gcggcgcgcc ggccccggcg cgccgcgggc aggagtcgac
     3541 ggcgtcggac ggatcagcgc gagccgaaca gcccgagcag gatctgcggc gacgcgttgg
     3601 cgatgccgag cgtctggacg gcgagctgct gcttgacctg aagcgcctgc agcttggccg
     3661 attccttcgc caggtcggca tccaccaggc cgcccaggcc ctcttccacg gcgtcggcga
     3721 tggtggccag gaagtcgtcc tggaagtcga gcttgcgggc gtccgcgccc agctggccga
     3781 gggcgacgtt gatcgtgttg accaccacgt tcacctgggt cagcgcggcc gaggcggcag
     3841 tgacgttggc gatgttctgg gcgttgagcg cgctgaaggc ggtgccctcc aggtcctggg
     3901 cgcggagcgt gatggcgccg ccgtcgatgt cgccgatgac gttgatcgcc gtcgagcccg
     3961 actggaggat gttgcggccg ttgtactcgg cgttctggat gaagctcagc acctgggtga
     4021 ccagggcggc gtagtcggcg tcgaggatcg cctgctgctc ggaggtgttg cccggattca
     4081 gcccctcgat caccttggcc ttgagctcga tcagcatgtt ggacaccgcg gtcgcgccgg
     4141 caatcgcgac cgcggccacg ccgcggccgt tggacaggcc ttgctggacc gcggcgatgc
     4201 ccttgatgtc gccgcggatg ccctgggcga tggcgaacga cgaggcgttg tccttggcgc
     4261 cgatgaccct gagcccggtc gacacccggt cctggacctt gctgagctgg gcgttggtca
     4321 ggttgaggta gcgcagcgcc accatcgcgc cgacattggt gttgatggaa ttctcgccgg
     4381 ccatcaccga actccttcgc tggcgtggtc aaaggccgtg ctcgtgctag caaggaccgt
     4441 gcccagcgcg cgtgccggca taaccttttg attcggcgga acgttttagg cagcgcgcag
     4501 gccccgctgg gggcggcggg cagaacttcg cgggccccgg gcaagacttg ccgggcggca
     4561 cggcctgggc actcatgcct cggcggggat agccgttggc gtggcaaccg atggcgcgac
     4621 gcgcggcccg gccggggtca cagcgatcgc aaaaaggcga gcagggcctc gcggtcgccg
     4681 cggggcaggg cacggaacct ctcgcgggcg gcctcggcct cgccgccgtg ccagaggatc
     4741 gcctcggcca ggttgcgggc gcggccgtcg tgcaggaaat aggtgtggcc gttgaccgtc
     4801 tcgaccaggc cgagtcccca caacggcgcg gtgcgccact cgcggccggt cgccgcgaag
     4861 tccgggcggc cgtcggcgag accttcgccc atgtcgtgga gcagcaggtc ggtgaacgga
     4921 tggatcacct ggccagcgag ctccgcgagc ggatgcgcgc cggtgaccag ggtcggcatg
     4981 tggcaggccg cgcagcccgc ggcccggaac agctgctcgc cgtggcgcac cggctcggcg
     5041 tcggcgcctc tttgccgcgg caccgccagc agccgggtgt acagggtgag cttgtcgagg
     5101 aacgcgtcgg acagctcggg cgcgccgccg ctctttgcgg cccggcaggc gtcctgcggc
     5161 gcggcgcagt tctcgtccgg gaacagggtg gaggtgaggc cgatgtcgcc gagcgcggcg
     5221 gcggcgttct gccggcgcag cgacggctgg ttcgccttcc agccgaagcg gccgagcgcg
     5281 atgccgccgc cgacctcgcc cgcgacccgg ttcggccggc cggagacgcc gtcgccgtcc
     5341 gcgtccgcgg ggtcggcgag cgccaggatc gtcgcctcgg gcaccgcctc gagcaatccg
     5401 agcccgatgg tcgccggggc gacgcgcggc gacagcatcg cgtcgtcgcc gagcgggccg
     5461 aaggcgag
//
LOCUS       DASWHU010000405         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3267505, whole genome shotgun sequence.
ACCESSION   DASWHU010000405 DASWHU010000000
VERSION     DASWHU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267505"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VGB88_14860"
     CDS             complement(<1..1064)
                     /locus_tag="VGB88_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9648784.1"
                     /translation="MTETGIGARVARKEDRRFLTGRGRYVDDINRPGQAYAWFVRSPH
                     AHARIKGIDAARAASSPGVLAVLTGADCAADGLGGLPCGWLVTGKGGKAMAEPPHPVL
                     AQGKVRHVGDPVAVVIAEGRARARDAAELVEVDYEPLAAVVDAVDALAAKRPQVHDEA
                     PGNLCYDWEFGDQAAVAAGFAKAERVVSLDLVNQRLVANAIEPRAAVGAYDAGEDRYT
                     LYTTSQNPHVIRLLMCSAVLHLPETKVRVVSPDVGGGFGSKIYHYAEEAVVTWAARKV
                     GRPVKWTAERSESFLSDAQGRDHATHVELALGAGAKFLAMRVSTTANVGAYLSTFGSA
                     IPTYFYAPLLAGVYTTPAVY"
     gene            complement(1067..1537)
                     /locus_tag="VGB88_14865"
     CDS             complement(1067..1537)
                     /locus_tag="VGB88_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9648785.1"
                     /translation="MSIAVSMTVNGKPTSGTVEARTLLVDYLRGNLGLTGTHVGCDTS
                     QCGACVVHMDGVSVKSCTVLAAQAEGATVVTIEGLANGEALHPMQEAFRDHHGLQCGF
                     CTPGMVMSAIDLATRHPDPSEELIRHELEGNICRCTGYHNIVKAVQAGAKAMGR"
BASE COUNT          233 a          592 c          610 g          254 t
ORIGIN      
        1 cagtagaccg cgggcgtggt gtagacgccg gcgagcagcg gcgcgtagaa gtaggtcggg
       61 atggcgctgc cgaaggtcga gagatacgcg ccgacattgg cggtggtcga gacccgcatg
      121 gccaggaact tggcgccggc gccgagcgcg agttcgacat gggtggcatg gtcgcgcccc
      181 tgtgcatccg acaggaacga ctcgctgcgc tcggctgtcc acttgaccgg ccggcctact
      241 ttccgcgccg cccaggtgac caccgcctcc tcggcgtagt ggtagatctt ggagccgaag
      301 ccgccgccga cgtcgggcga gacaacccgc accttggtct ccggcaggtg cagcacggcg
      361 ctgcacatca gcagccggat gacgtgcggg ttctggctgg tggtgtagag ggtgtagcgg
      421 tcctcgcccg catcgtacgc gccgaccgcg gcgcgcggct cgattgcgtt cgccaccagg
      481 cgctggttga ccaggtcgag gctgacgacg cgctcggcct tggcgaagcc ggcagccacc
      541 gccgcctggt cgccgaactc ccagtcgtag cacaggttgc ccggcgcctc gtcatggacc
      601 tggggccgct tggcggcgag cgcatcgacc gcgtcgacca ccgccgccag cggctcgtag
      661 tcgacctcga ccagctcggc cgcgtcgcgc gcgcgggcgc ggccctcggc gatcaccacc
      721 gccaccgggt cgccgacatg gcgcaccttg ccttgggcga gcaccgggtg cggcggctcg
      781 gccatcgcct tgccgccctt gccggtgacc agccagccgc agggcaggcc gccgagcccg
      841 tcggcggcgc agtccgcgcc ggtcagcacc gccagcaccc cgggcgagga cgcggcgcgg
      901 gcggcgtcga tgcccttgat ccgggcatgg gcgtggggcg agcggacgaa ccaggcatag
      961 gcctggccgg ggcggttgat gtcgtcgacg tagcggccgc gcccggtcag gaaccggcgg
     1021 tcctccttgc gcgcaacacg cgccccgatg ccggtctcgg tcatggtcac ctccccatcg
     1081 ccttggcgcc cgcctgcacc gccttgacga tgttatggta gccggtgcag cggcagatgt
     1141 tgccttcgag ctcgtggcgg atcagctcct cgctcgggtc cgggtggcgg gtggccaggt
     1201 cgatcgcgct catcaccatg cccggggtgc agaatccgca ttgcaggccg tggtggtcgc
     1261 ggaacgcctc ctgcatcggg tgcagcgcct cgccgttggc cagcccctcg atggtgacca
     1321 ccgtcgcgcc ctcggcctgg gcggcgagca cggtgcacga cttgaccgag acgccgtcca
     1381 tgtggaccac gcaggcgccg cactggctgg tgtcgcagcc gacatgcgtg ccggtgaggc
     1441 cgagattccc gcgcagatag tcgaccagca gcgtgcgcgc ctcgaccgtg cccgaggtgg
     1501 gcttgccgtt gacggtcatg gaaaccgcga tggacatgtg tggccctccc cctcattcgc
     1561 gcccgcgccc gaacgcgcgg cgattgtgcg agccgcgccg aggcaggtca agcgggcgcc
     1621 ggcggcgccg gtgtcccagt ttgaaatcag tcgatattag gaacggcgga taaatcgccc
     1681 cgtcagtgc
//
LOCUS       DASWHU010000406         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2183092, whole genome shotgun sequence.
ACCESSION   DASWHU010000406 DASWHU010000000
VERSION     DASWHU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183092"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGB88_14870"
     CDS             <1..459
                     /locus_tag="VGB88_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX9648786.1"
                     /translation="AATPDGTVYYASLAGSYVGRIDPATGAAEVLEPPTAGQGARRVW
                     SDSKGMVWVSEWNSGQLSRFDPQTGAWHAWRLPGDSPRAYAVYVDDRDQVWLSDFSAN
                     ALVRFDPDSEAFTAYPLPSRGAAVRQIHGRPGEVWGAESGTDKLVVIRTR"
     gene            564..902
                     /locus_tag="VGB88_14875"
     CDS             564..902
                     /locus_tag="VGB88_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9648787.1"
                     /translation="MKKIEAIIKPFKLDDVKQVLSDEGVHGMTILDAKGFGRQKGHTE
                     LYRGAEYVIDFMPKVKIEVVVDDEQVERLVEVIRAAAHTGKIGDGKIFVVPIEHALRI
                     RTGETGSSAL"
     gene            958..2259
                     /locus_tag="VGB88_14880"
     CDS             958..2259
                     /locus_tag="VGB88_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9648788.1"
                     /translation="MRIRGVLGAALAAPLAVSASAALAQDGAAVDTGSTAWLLAATAL
                     VLLMTMPGLALFYAGLVRAKNVLSVMMQCFTICCLVSVMWLVFVYSLAFGDGGGANPW
                     IGGLGKAFLAGIGVDTVAGEIPETVFFMFQMTFAIITPALIVGAYVERIKFGAVVLFS
                     GLWLVLVYAPVAHWVWGGGWLAGMGVQDFAGGLVVHLTCGTSALVMARVLGSRSGFPH
                     EVSPPHSPGLVMAGAAMLWVGWYGFNAGSALAAGGGAGMAMTVTHISASTAALTWMAI
                     EWIRFGKPTLVGIATGAIAGLATITPASGFVGPLGGLVFGLLAGSVCFYAVGVIKSRL
                     QIDDALDVMAVHGVGGAMGTLLTAVFGVVALGGLGLSATGLGGQFGIQLVGVLATLVW
                     SAVLTFVIIKVVTALVGLRVEREVEIEGLDVTVHGETGYTL"
     gene            2293..3348
                     /locus_tag="VGB88_14885"
     CDS             2293..3348
                     /locus_tag="VGB88_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9648789.1"
                     /translation="MRGVVFLGERELALEEFPDPVPGPGEVVIEVKASGMCGSDLKFY
                     RAPRDGGGAAALGLGGGGGPVIAGHEPCGVIAALGPGNEATPFRVGARVMVHHYLGCG
                     HCEHCDVGWSQLCKHGITVYGVTGHGAHAPYMKVPARTLVALPDALSFETGAAIACGT
                     GTAFAALGRLGLSGRDTIAIFGQGPVGLSATQLAAAMGARVLALDISPERRALAAEFG
                     AHATIDPAAGDAVAVLAELTAGRGVDLALDCSGAPAAREAAVRATRTWGTCCFVGEGD
                     TVTLDVSRDMLRRQLTLIGSWTFSRIGQGECARFVAERGIAVDRLFSHRFRLDQAVDA
                     YRLFDRQTMGKGVFLPA"
     gene            3359..4204
                     /locus_tag="VGB88_14890"
     CDS             3359..4204
                     /locus_tag="VGB88_14890"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9648790.1"
                     /translation="MARLDGKVAIVTGATSGIGRRIAELFVAEGAHVALAGRRAELGG
                     EIAAGLGEAATFIATDVADELQVEALVRATLDKWQHIDCLVNNAGGPGPTGGIETLPI
                     EGVRAAFRVLFDGVLLGMKHVAPVMKRQGTGSIINIGSVAAHQAGYSSSMVYSGAKAA
                     MIHFSRCVAMELGESGVRVNCISPGAIATGILPKALGLPTEQAEAKVGQIAEMFAAMQ
                     PIRRAGLPDDIARCALYLAGDEAGFINGADFVVDGGLIAGRQWTPQQEGIKAMRAALG
                     APAGE"
     gene            4229..5023
                     /locus_tag="VGB88_14895"
     CDS             4229..5023
                     /locus_tag="VGB88_14895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9648791.1"
                     /translation="MDLDLSGRTALVTGSTRGIGFATACGLARMGASVIVHGQSDASV
                     EGALARARKAVAGATFAGVSGDLAAAAGCDRVKAAHGHVDVLVNNVGIYDFGPFEQVP
                     DETWLHFFQVNVMSGVRMARHYLPGMIARNWGRVVFVSSESGAFIPPDMILYGITKAA
                     QLALSRGLAETTAGTNVTVNAVLPGPTWVEQQEEAIAKRAASEGVSIDEAKKNTFRVR
                     RPTSLIRRYALPEEVANMICYACSPAASVTNGAALRAEGGIVRSIV"
BASE COUNT          686 a         1804 c         1955 g          774 t
ORIGIN      
        1 gccgccacgc ccgacggcac ggtctactac gcctcgctcg ccggcagcta tgtcgggcgc
       61 atcgacccgg cgaccggcgc cgccgaggtg ctcgagccgc cgaccgccgg ccagggcgcg
      121 cgccgggtgt ggtcggactc gaagggcatg gtctgggtct ccgagtggaa ctcgggccag
      181 ctctcccgct tcgacccgca gaccggggca tggcacgcgt ggcggctgcc gggcgactcg
      241 ccccgggcct acgcggtcta cgtggacgac cgcgaccagg tgtggctgtc cgacttctcg
      301 gccaatgcgc tggtccgctt cgaccccgac agcgaggcgt tcaccgctta tccgctgccc
      361 agccgcggcg ccgcggtgcg ccagatccac ggccgcccag gcgaggtgtg gggcgccgag
      421 tcgggcaccg acaagctggt cgtgatccgt acccgctgac ccggcggcgc gatgcgccct
      481 tccgccggcg cccggattcg ggttaggctt tggcggcggc cggtcccggg gcgccggcgc
      541 ggctgcggcg gggggttgca ggcatgaaga agatcgaggc catcatcaag ccgttcaagc
      601 tggacgacgt caagcaggtg ctgagcgacg agggcgtgca cggcatgacc atcctcgacg
      661 ccaagggatt cggccgccag aagggccata ccgagctcta ccgcggcgcc gagtacgtga
      721 tcgacttcat gcccaaggtg aagatcgagg tggtggtgga cgacgagcag gtcgagcgcc
      781 tggtcgaggt catccgcgcc gccgcgcaca cgggcaagat cggcgacggc aagatcttcg
      841 tggtgccgat cgagcacgcg ctccgcatcc gcaccggcga gaccggatcg tccgcgctct
      901 agctctgggg ggatgcgcgc gggcgctgcc ggcgcgcagg cgagggagag ggaggcaatg
      961 cgcattcgcg gagtgttggg ggcggcgctg gcggcgccgc tggcggtgtc ggcgtcggcg
     1021 gcgctggccc aggacggggc tgcggtcgac accggcagca cggcctggct gctggcggcg
     1081 acggcgctgg tcctcttgat gaccatgccg ggcctggcgc tgttctacgc cggcctggtc
     1141 agggccaaga acgtgctgtc ggtgatgatg cagtgcttca ccatctgctg cctggtgtcg
     1201 gtgatgtggc tggtgttcgt ctacagcctg gcgttcggcg acggcggcgg cgccaacccg
     1261 tggatcggcg gcctgggcaa ggcgttcctc gccggcatcg gcgtcgatac ggtcgcgggc
     1321 gagattcccg agaccgtgtt cttcatgttc cagatgacct tcgccatcat caccccggcg
     1381 ctgatcgtcg gcgcgtatgt cgagcgcatc aagttcggcg cggtggtgct gttctccggc
     1441 ctgtggctgg tgctggtcta cgcgccggtc gcgcattggg tgtggggcgg cggctggctc
     1501 gccgggatgg gagtccagga cttcgccggc gggctggtgg tgcacctcac ctgcggcacc
     1561 tcggcgctgg tgatggcgcg cgtgctcggc agccgctcgg gcttcccgca cgaggtctcg
     1621 ccgccgcaca gcccgggcct ggtgatggcc ggcgcggcga tgctgtgggt tggctggtac
     1681 ggcttcaacg ccggcagcgc gctcgccgcc ggcggcggtg cgggcatggc catgaccgtc
     1741 acccatatct cggcctcgac cgcggcgctc acctggatgg cgatcgagtg gatccggttc
     1801 ggcaagccga ccctggtcgg catcgccacc ggcgcgatcg cgggcctggc cacgatcacc
     1861 ccggcctctg ggttcgtcgg gccgctcggc ggcctggtgt tcggcctgct cgccggcagc
     1921 gtgtgcttct acgcggtcgg cgtgatcaag tcgcgcctgc agatcgacga cgccctcgac
     1981 gtgatggcgg tgcacggggt cggcggcgcc atgggcacgc tgctcaccgc ggtgttcggc
     2041 gtcgttgcgc tcggcgggct cggcctgtcc gcgaccggcc tcggcggcca gttcggcatc
     2101 cagctggtcg gcgtgctggc gacgctcgtg tggtcggcgg tgctgacctt cgtgatcatc
     2161 aaggtggtca ccgccttggt cggcctgcgc gtcgagcgcg aggtcgagat cgagggcctc
     2221 gacgtcaccg tccacggcga gaccggctac accctgtagc gcgccgtcgg ccgggcagac
     2281 aaggaggcgt cgatgcgcgg agtggtgttc ctgggcgagc gcgagctcgc gctcgaggag
     2341 ttcccggacc cggtgcccgg cccgggcgag gtggtgatcg aggtcaaggc ctcgggcatg
     2401 tgcggcagcg acctcaagtt ctaccgcgcg ccgcgcgacg gcggcggcgc cgcggcgctc
     2461 ggtctcggcg gcggcggcgg gccggtgatc gccggccacg agccgtgcgg ggtcatcgcc
     2521 gcgctcgggc ccggcaacga ggcgacgccg ttccgcgtgg gcgcgcgggt gatggtccac
     2581 cactatctcg gctgcggcca ttgcgagcat tgcgacgtcg gctggtcgca actctgcaag
     2641 cacggcatca ccgtctacgg ggtcaccggc cacggcgcgc acgcgcccta catgaaggtg
     2701 ccggcgcgca ccctggtggc gctgcccgac gcgctgtcgt tcgagaccgg cgccgcgatc
     2761 gcttgcggca ccggcaccgc gttcgcggcg ctgggccggc tcgggctgtc ggggcgcgac
     2821 acgatcgcga tcttcggcca ggggccggtg ggcttgagcg cgacccagct cgccgcggcg
     2881 atgggcgcgc gcgtgctcgc gctcgacatc tcgcccgagc gcagggcgct cgccgcggag
     2941 ttcggcgccc atgccaccat cgatccggcg gcgggcgatg cggtggcggt gctcgccgag
     3001 ctcaccgccg ggcgcggcgt cgatctcgcg ctcgactgca gcggcgcacc cgccgcgcgc
     3061 gaggccgcgg tgcgcgcgac ccggacctgg ggcacctgct gcttcgtcgg cgagggcgac
     3121 acggtgacct tggacgtgag ccgggacatg ctgcgccgcc agctcaccct gatcgggtcg
     3181 tggaccttca gccgcatcgg ccagggcgag tgcgcgcgct tcgtcgccga gcgcgggatt
     3241 gcggtcgacc ggctgttcag ccaccgcttt cggctggacc aggcggtgga cgcctatcgc
     3301 ctgttcgacc gccagacgat gggcaagggc gtgttcctgc cggcgtgagg gaggcgagat
     3361 ggcaaggctc gacggcaagg tcgcgatcgt caccggcgcc accagcggca tcggccggcg
     3421 catcgccgag ctgttcgtcg ccgagggcgc gcacgtggcg ctcgccggcc ggcgcgccga
     3481 gctcggcggg gagatcgccg ccggcctcgg cgaagcggcg acgttcatcg ccaccgacgt
     3541 cgccgacgaa ctccaggtcg aggcgctggt ccgggccacg ctcgacaagt ggcagcacat
     3601 cgattgcctg gtcaacaatg ccggcgggcc ggggccgacc ggcggcatcg agaccttgcc
     3661 gatcgagggc gtgcgcgcgg cgttccgggt gctgttcgac ggcgtgctgc tcggcatgaa
     3721 gcacgtcgcg ccggtgatga agcgccaggg gacgggcagc atcatcaata tcggcagcgt
     3781 cgccgcccac caggcgggct actcgtcgtc aatggtctat agcggcgcca aggcggcgat
     3841 gatccatttc agccgctgcg tcgccatgga gctcggcgag tccggggtgc gcgtcaattg
     3901 catctcgccg ggcgccatcg ccaccggcat cctgcccaag gcgctgggcc tgccgaccga
     3961 acaggcggag gcgaaggtcg ggcagatcgc cgagatgttc gccgccatgc agccgatccg
     4021 ccgcgccggc ctgcccgacg acatcgcccg ctgcgcgctc tatctcgccg gcgacgaggc
     4081 gggcttcatc aacggcgccg atttcgtcgt cgacggcggc ctgatcgccg gccgccagtg
     4141 gacgccccag caagagggca tcaaggcgat gcgcgccgcg ctcggcgcgc cggccggcga
     4201 gtagccggag accagggagg ggcgcgacat ggacctcgat ctttccgggc gcaccgcgct
     4261 ggtcaccggc tcgacccgcg gcatcggctt cgccaccgcc tgcgggctcg cccgcatggg
     4321 cgccagcgtg atcgtccacg gccagagcga cgcctcggtc gagggcgcgc tcgcgcgcgc
     4381 gcgcaaggcg gttgcgggcg cgaccttcgc gggcgtctcc ggcgaccttg ccgccgccgc
     4441 cggctgcgac cgggtcaagg ccgcgcacgg gcacgtcgac gtcctggtca acaatgtcgg
     4501 catctacgac ttcggcccgt tcgagcaggt gcccgacgag acctggctgc acttcttcca
     4561 ggtcaacgtg atgagcggcg tgcgcatggc gcgccactac ctgcccggca tgatcgcccg
     4621 caactggggc cgggtggtgt tcgtctcgag cgagtcgggc gcgttcattc cccccgacat
     4681 gatcctctac ggcatcacca aggcggcgca gctcgcgctc agccgcggcc tcgccgagac
     4741 caccgccggc accaacgtca cggtgaacgc ggtgctgccc ggcccgacct gggtcgagca
     4801 gcaggaggag gcgatcgcca agcgcgcggc gagcgagggg gtgagcatcg acgaggcgaa
     4861 gaagaacacc ttccgggtgc gccggccgac ctcgctgatc cgccgctacg cattgcccga
     4921 agaggtcgcc aacatgatct gctatgcgtg cagcccggcg gcgtcggtga ccaacggcgc
     4981 cgcgctccgc gccgaaggcg gcatcgtgcg cagcatcgtg tgaacgtgct aaactgtact
     5041 cggccacacg gcggaaggga gagcaggaca tgaccgaccc gcgcctcgca ggcaagccga
     5101 tccccagggt ctcggtcgcg cgcatggaac gcgaccacct ggcgcgcgtc gcggcgatga
     5161 agggctcgaa gctcgctttt ctcgaccagc gcatgccggg gcatgagcgc gagatcatc
//
LOCUS       DASWHU010000407        19280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_305607, whole genome shotgun sequence.
ACCESSION   DASWHU010000407 DASWHU010000000
VERSION     DASWHU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19280
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305607"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VGB88_14900"
     CDS             complement(<1..372)
                     /locus_tag="VGB88_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX9648792.1"
                     /translation="MAEDFTLDGKSILVTGGTGSFGQRFVQRVLERFKPRRLVVLSRD
                     ELKQYEMSQRFSERDYPCLRYFLGDVRDRERMARACATVDIVVHAAALKQIPAAEYNP
                     TECIATNIMGAQNVIDAAIAND"
     gene            complement(377..985)
                     /locus_tag="VGB88_14905"
     CDS             complement(377..985)
                     /locus_tag="VGB88_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9648793.1"
                     /translation="MTADSIMSGSAGNRVRESEIGAVRLAPYFPEHVTATSVAWLNDP
                     DVVRFTEARFDSHTLDSQRAYVAAAARSPAARLWRIMVEGEGHVGTIRLSSIDRRHRH
                     ARVALIIGRKDLWGQGIAQRAIAAACTVAFDELGLRKLLAGIYASNAPSLRAFVKAGF
                     VEEARLARQYFDGEDWVDGVIMSRFAGAEGGQLPRQEIQPAR"
     gene            complement(985..2031)
                     /locus_tag="VGB88_14910"
     CDS             complement(985..2031)
                     /locus_tag="VGB88_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9648794.1"
                     /translation="MTRSRNGAPVAASLSQTLITGTYRSGTELLSLLLSSHPELSSTM
                     YHVNVLRFCVGRYDPIAQAGRLRCALADLGVRLADRYRIELDTDRAFDACLARAPVTY
                     GVLYDVVMTQLWLAGRRRHWAEKCQLLWREIPAFLSMMPNGRAILVVRDPRSVLASFK
                     RYTSAPGKRYLEAVFNAYDAMKAALDYAGRLAEDRFRMIRYEDLAAAPEAAAAGLLEF
                     LGLDPARADFSRARWRDAYGRSWRANSAFGADGADFDVAASIDRWRGNLTDAEIALCE
                     WVTGEQMAAFGYRRCRPEVEPAEVVRLALQDPAIAEHLRTWALTGRGIEAFPSDPLLP
                     ENWERPTAVAARSA"
     gene            complement(2028..2849)
                     /locus_tag="VGB88_14915"
     CDS             complement(2028..2849)
                     /locus_tag="VGB88_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648795.1"
                     /translation="MTERRFHYVTPLIWPVLPATYGALEIGLPIQKILDSRMDCPYDG
                     PLRIDFTLHVDGERRSEDRVTCQVAGGRADPRDVWRRISHDRLGYLEAALSCDAPVFR
                     RIDVSVGYATLIGAHGSLNLIPDYKYARPIIIEQISGINRFCLMHAAALVDRRRDSGN
                     AYLMINPYSADIVVRMRTAAGRTMRRRLAPRHATIVPLDELVEDGEITAVMMTANNRF
                     LAWDLRYALDDPTAINSIDHLEAFRGQATHRRVRLTDALKATGRRGLDAMGLRVS"
     gene            complement(2852..3796)
                     /locus_tag="VGB88_14920"
     CDS             complement(2852..3796)
                     /locus_tag="VGB88_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9648796.1"
                     /translation="MTGSERSARAAGARTLCFCFDVTEAAVRAHFARGGDYDTLVERT
                     GIGTRCTACLLDLDVVLDRIHQARTPGPATVSGPAAATQAGLLVRPTDWTDSGFLVCD
                     AGIKTVLRIVNHPLLFEADWPVPVYDYSLRLFDETGRARACRSGFLGAGAELSYELAT
                     MPGCPPRGWFLIRLRPRSRGVFGSIRPYVVLEGPTWTTAYHTQPQAMASTLHHRTRVA
                     IAGNGKRLAAAVSLINAARARTTVCLKLSDPGGAHAAEAELSLDGHASTIIDLDQLFT
                     DMPCDRALTLEVTSTQPTRRHIIGYQANGAWNVDHFPN"
     gene            complement(3807..4565)
                     /locus_tag="VGB88_14925"
     CDS             complement(3807..4565)
                     /locus_tag="VGB88_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9648797.1"
                     /translation="MRGQGGLAWRALPALRLLFRAAGRSWNDFYAWMLDRQERANSID
                     SIIGRGRSHPGADKGLYDLSRGRYHAEYLKRHGLRPEHRLLDIGCGYGRTAVAVLPYL
                     EPGHYIGTELSRQRLRLARELVARQGLCDRDPRFVLSIDNAMPELDAACVDVIWAQSV
                     FTHMPRAEAHMLLAACRRVLVPGGRMLFNYASSPDDAVHERNVKDFCYPDRVMAAMVR
                     SAGFALEVMDDWQDDLQPQHRARHNRMLKLTARD"
     gene            complement(4597..6171)
                     /locus_tag="VGB88_14930"
     CDS             complement(4597..6171)
                     /locus_tag="VGB88_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648798.1"
                     /translation="MTTVVEALGWAAGTEVAAAAPRCVFLSPLSLYDWLDEAVMTIMR
                     RRYGTRFLVLVGGPRQRDMCAQWATDKDRIFDLGEIERQALAAAGPADADSVFAEARR
                     LEAVHGVVYLRDIVQQDRAIYGAYLAYAAGPLANSRPPALADIYRRINGYFRLFQQLY
                     QAHRVDLVIGRPGNLLNTTSIVLALAIGLPVTMPRHARQRSDVMWSFGPYSNPHLLAR
                     TYQRLADQPLVPLDEVRPPTSSQRTFSWFQWRYTARGVAKELLVLAHTYAVKYARGLR
                     HDGRLPAMQPFHRAAGAVLSSARLARWMARSADGDLDRLAAVPFLFFPLPKEPEYTVQ
                     SLARHFSNVQAMAEQLALSLPAGFRLVIKEHSRLGDRRRQFYQDLLKLPNVAMADPNI
                     PGVELAARARAVATIAGTAAFEALLLGKRAIVFATDVEYGFLPAVARVTSFADLPAVV
                     SEALREVDDAEALAIRQSAARLRRAYAELAFDASGTRAFGGQTPLAPAQAERAVDLLV
                     ATCLAERDYLARGGKL"
     gene            complement(6168..7136)
                     /locus_tag="VGB88_14935"
     CDS             complement(6168..7136)
                     /locus_tag="VGB88_14935"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9648799.1"
                     /translation="MTGSGRRGDAGQPFRPLLISGPPRSGTTLVYSLFDGHPDIFSLV
                     TEGYLFEYLHDLPDGGVAAFIDAGRLPAAEFVAGLRAKDVMPPLHRPVRLGTTNPAIA
                     KETATLDWSEDRFLAALDVGRAASVGELWYGLARAYAAALGRPPARYCSMKAPDYGKS
                     AMAATSHIAEAKAIVVVREPIRALDSLKRVREMRGLREKLLTWPVLAQCVAEMNALAE
                     RLHRADPDRVTWLRFEDLTARPEATMRRIAAWCGLDFQPSLTRPSVLGRDIPSNSSFE
                     AVLDIQDSVATRGVRRLSAGEIDFACRNLGRFLNFFGYAPAHADVP"
     gene            complement(7133..8989)
                     /locus_tag="VGB88_14940"
     CDS             complement(7133..8989)
                     /locus_tag="VGB88_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9648800.1"
                     /translation="MLARALHLITGILPAELREEIRILRGYRLVGLTDAGVIATTGIL
                     ALAFEMFSVAMIYPLLDYIEGGRDLAVLAERSRLWVVIGKVADAVHMPVTLGSLAAVI
                     VALVLCRQVANYAHVMQVTRIKQRVGRDLSRLCFSAVMGSNALFMKEMNAGRFVNILD
                     HQCQHVASLVRSYAAIWQMMLSFIAYGGVMVATAPGASLLALLVAAAVVMSLGRYMHL
                     SRRVSREVVKLRQRFCAFMAERHQGWQVIKVSDSLDREARSFGDWAQQFFRLSVDLNR
                     LAGRMQLVIAPAMTAFALSALYVGVEYLDLSLSIITLFILMILRMVPIAQGLTNHRQA
                     LITHHAGLEEIVEVVSQARAHKEPDRGTIPFRGLERAIEFRGVWFSYPSGRQAALRDV
                     SVRIPAGVTTALMGPSGAGKSTLADMIPRLIVPDRGEISIDGVPLNSFSLSSLRRGIS
                     FAPQQNFLFNASVADNVRYARPSASDAEVEEACRLAYAHDFIVELPERYDTLVGEAGS
                     RLSGGQRQRLVLARAFLSRASILILDEPTSELDYESERKIRAALENMHANAHMTIVVV
                     AHRMSTVRNADHVIVLSDGRVLEQGSPAELIRDTQWYGAMLDLSARDAMVDP"
     gene            complement(8990..9679)
                     /locus_tag="VGB88_14945"
     CDS             complement(8990..9679)
                     /locus_tag="VGB88_14945"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEX9648801.1"
                     /translation="MARPTLLIVVPARGGSKRLPGKHLRPLCGRNLLAHADTAIGEAA
                     LDAPVVLSTDDAAIAAEGRRLGWLVPFVRPRHLSGDDSASVDMLLHALDAFGRSAGYA
                     PELVMLLQVTSPLRPAATLAEAVALLEARADADAVVGVRALERTPQTVFRLGADGFLA
                     PAGADSAPLYTPNGALYVIRTEIVRRRRSLFPERTLPVVMDARASLDIDTELDWRMAE
                     YLLAQPVTAAS"
     gene            complement(9684..10943)
                     /locus_tag="VGB88_14950"
     CDS             complement(9684..10943)
                     /locus_tag="VGB88_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEX9648802.1"
                     /translation="MTTITRGEESFVIDPEDGRLEVKYGLPPKVEFCRTCVISNQRPS
                     STIEFAHRPDSKKTTIRFDADGVCDACRYAETKRTAIDWDERARALRELCDRFRSQNG
                     SYDCLVPGSGGKDSVYASHVLKTSYGMHPLTVTWAPHLYTDIGWRNFQGWIHRGGLDN
                     YLFTPDGQVHRTLTRLAFRNLLHPFQPFILGQKNFAPKMALRFGVPLIFYGENEAEYG
                     NPIDENASALRGADYFARRRMWNRDIYLGGVPIDELPRHGVAPGRIEPYLPADLNALD
                     AAQIEVHYLGYYLSWTPQQNYYYAVEHTGFCTNDERTEGTYSKYNSIDDKTDPFHYWT
                     TLVKFGIGRATYDAAQEIRNQHITREEGVALVHRYDLEFPRKYFAEFLDYLDMDETTF
                     FAVADRFRSPHLWKKVGSDWRLRHMVS"
     gene            complement(10954..11913)
                     /locus_tag="VGB88_14955"
     CDS             complement(10954..11913)
                     /locus_tag="VGB88_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9648803.1"
                     /translation="MSGLRTSAGAAVAEATLARLRCLDCHAGGLLSEARGERLRCAAC
                     GASFPVNGGVPSLLRGGADSQPWNPWDLDQVQKMGDSYYKRAKGELPEKEASKSFASF
                     LARTGIYRPGASILDLGAATGHFVRSFRRLLDDEVDYTGLDITARYLAWGAEIFGTGA
                     RTAFVHADVLDMPFVDDAYDIVAVNLFHFFPRIDAALGEAMRVARKWVVWRQPIGPCN
                     YAVKVIYDQDFDRVGVITPEREDFDFSLYMIYSRPYLEGLVRHLGGRIDRFERDTDFA
                     PFDNTALAEFDGIPATKVVGDMQINGALVLDWHYLAIACPGKE"
     gene            complement(11910..12539)
                     /gene="hisH"
                     /locus_tag="VGB88_14960"
     CDS             complement(11910..12539)
                     /gene="hisH"
                     /locus_tag="VGB88_14960"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9648804.1"
                     /translation="MIAVVDFGRGNLLSIAQALRHCGAGEVEITDDAEAVLAAERIVL
                     PGVGAFADAMRALQARGLVAALRQAAARGTPMLGICLGMQLFADESEEFGRHDGLGLV
                     AGAVRRLPAGNGPGAIRIPNVGWRALEVRRPDPIAAGLAEGAMMYFVHSYAHAPADAG
                     DVVATIAVNGVQVAAMVRKGGLIGCQFHPEKSGETGLAVIRNFVALDRS"
     gene            complement(12536..13294)
                     /locus_tag="VGB88_14965"
     CDS             complement(12536..13294)
                     /locus_tag="VGB88_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HEX9648805.1"
                     /translation="MTPIRIVARLDVKGPNVIKGVHLEGLKVVGDPGEMARRYYVEGA
                     DEIIYMDVVASLYQRNNILSVVEQAAHDVFIPLTVGGGLRGIDDIKAALRSGADKVAI
                     NTAAVRRPQFITEAAETFGSQCVVLSVEAKRRAEGTWEALVDNGRERTGRDVLDWVVE
                     GEGLGAGEILVTSVDAEGTRGGFDVDLLTEICGRVRVPVIACGGAGGADDIAALLARA
                     PCDAVCCASIFHDRSCPVGEVKERLSSAGIEVRR"
     gene            complement(13291..14679)
                     /locus_tag="VGB88_14970"
     CDS             complement(13291..14679)
                     /locus_tag="VGB88_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648806.1"
                     /translation="MTAKVYIAHCIDTEGPLYESLDARFERLSELFAIDDLPRTAETL
                     RKLRDKEIALGGIEEAVALTLSSHRNAYLESWHQVDAMLERVTAPAFRDRLPDSAGNG
                     WVFTWFCLDHVGYDYNPRRRDIGFHNVFDHYARRLAAAPGCPDELQWHFHPMSTYGDA
                     HRCATHYFRTDHVFQVLCRKVIERAWFPSTFRAGFQAERPDSHWFLEQWIPFDISSMA
                     LDDNRALDDTIDFRNGRSGDWRLAPADWGIYHPSHDDYQRPGGCRRWIGRALNVMNRI
                     GNLDQGEMDMAFARADAGRPALVGVASHDFRDLGPEIDHVRAMIAESRRRFPTVAFEY
                     VRASDGFRRMIGAPADGAEPLALDLAFHPADRSDVARIEVRTAAGEVFGPQPFLALET
                     RSRRFIHDNFDFSPCRRRWHYAFHADTLPIEDVLRVGVAANDRFGRTCIRRLEFQSDR
                     ARPVAVLDAAAA"
     gene            complement(14676..15842)
                     /gene="neuC"
                     /locus_tag="VGB88_14975"
     CDS             complement(14676..15842)
                     /gene="neuC"
                     /locus_tag="VGB88_14975"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX9648807.1"
                     /translation="MTVRIAAVSSSRADSGIQSAVWRALVETAGTDLHVLLTGMHCRD
                     DAAEAETPAAATTHRGGADLGGTEPAAAAAMAAIAAFAGEVYRRIRPDIVLVTGDRLD
                     MLPAAAATLPFNLPVAHLHGGEISAGAVDDRIRHALTKLAHLHFVATAAAARRLAQMG
                     EELWRIHLTGAPGLDRLRAAPRLAADALAAELDLPTVAGLRLVTVHSETNSSDPLAPL
                     DAVLAALDADPAPTLFTAANSDPGGSAIAERVADFVAARGWAQYLATAGTTLYANLLR
                     QAAVMVGNSSSGLIEAPLFGLPVVNVGGRQEGRERGANVIDCAAEAAAVRTALARAAA
                     MPRRPGASPYGDGFSAPRIAALVAGLPERATLLAKRFAAPGAPAPEEIEAALRA"
     gene            complement(15839..16405)
                     /locus_tag="VGB88_14980"
     CDS             complement(15839..16405)
                     /locus_tag="VGB88_14980"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX9648808.1"
                     /translation="MDVLLIGAGGHGRVVADALRAAGHRISAYADPRPSAWLDAVQLD
                     DDQALATAAGHPVAVGLGGVRPEDLESRLAIVHRLVAARAGLAPVIHPAATVSADAVL
                     GAGTVVMAGAVIQSGARLGMAVIVNTGAVVEHDAEIADGAHVAPRATVLGGAQVGACA
                     MIGAGAVVLPGARVPAASLVPALTRYPT"
     gene            complement(16390..17550)
                     /locus_tag="VGB88_14985"
     CDS             complement(16390..17550)
                     /locus_tag="VGB88_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9648809.1"
                     /translation="MMERKYDIPLSVPDLRGREKEYLLSCVDDNWVSTAGPFVDEIEA
                     RLPALAGCERAVATSSGTAALHLALLALGVKAGDTVIVPDWTFGATANAVYYTGARPL
                     LVDVSADSWTLDPGLVNELLAEQEFAAVIAVDALGHPADMDPISAACRAHGVPLVEDA
                     AGAIGARYRGHSAGGLGDIGVFSFNGNKTVTAGGGGAALTSDSQLAETMRMLSVHRLT
                     DAGRTPTEFYRYAVVGFNYRMTNINAAVGLAQLERLDEMLARKRSIAAAYDRAFAART
                     DLVPMPRAAWADSSCWMYSVSCGCARDAADLIRYLESRRIQARVFWQALSSQPAYRDA
                     PRRLTGVSRLIGERVVALPCSTGLTPAQQDRVIDAVAAWQGSAAVERAWMSC"
     gene            complement(17547..18626)
                     /locus_tag="VGB88_14990"
     CDS             complement(17547..18626)
                     /locus_tag="VGB88_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9648810.1"
                     /translation="MSLVRLTDFAAIVCAPDTTVRRAMERMTETGNLFQVVADRTGRV
                     LGTVTDGDVRRAILSGVAVEESIVRIMQREPVIGRIDRLDANRDLLEWLRRASPHAAF
                     LPVVDESGTLREILAFADVESEPPAALIMAGGLGRRLGERTRDLPKPLLPVGGKPIVE
                     RLIARLEETQVRRVYIAVHYLADKIAEFAAGRPGIAELSILREDEPLGTAGAIGRIDD
                     LPAGGLLVLNGDVLTDVDYGALVSFHRRNRHDGTVAVAQYQVDVPYGVVRHNDDGLFL
                     GIQEKPCMHYFVAAGIYLLSPEFCALVRPDQRLDMPDLMELGRGAGLRVGLFPIHEYW
                     KDLGRPQDLETAERDLDRVANRERE"
     gene            complement(18623..>19280)
                     /locus_tag="VGB88_14995"
     CDS             complement(18623..>19280)
                     /locus_tag="VGB88_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648811.1"
                     /translation="RDNAGRWFDAEFRRLELPASEQIVVYMLGFLGPLDFLRSERSGT
                     ELLHATLTVLADVAKSRPVFIKPHAVTDMALVKHALARFSDARFAVSYLHPSVLATRA
                     ACFVTNYYSTTQADALVMGVPTIEFSDYADAFLELTGHRSLNPAYTTHFVNRDADAFR
                     RTLAAVLGGAARPAPAPALEDDSGIIRRLAFGARAGRMASPETATRGIEEAQRRRHAS
                     "
BASE COUNT         2996 a         6679 c         6827 g         2778 t
ORIGIN      
        1 gtcgttggcg atcgcagcgt cgatcacgtt ctgcgcgccc atgatgttgg tcgcgatgca
       61 ttcggtcgga ttgtactccg cggccgggat ctgcttgagc gcggccgcgt gcaccacgat
      121 atcgacggtc gcgcaggcgc gggccatgcg ctcgcggtcg cgcacgtcgc cgaggaagta
      181 gcgcaggcac gggtaatcgc gttcgctgaa ccgctgcgac atctcgtact gcttgagctc
      241 gtcgcggctg agcaccacca ggcgcctggg cttgaagcgt tccagcacgc gctggacgaa
      301 gcgctggccg aacgagccgg tgccccctgt gaccaggatc gatttgccgt cgagggtgaa
      361 gtcctcggcc atcacgtcac cttgcgggct gaatttcttg gcgtggcaat tgacctccct
      421 cggcgccggc gaagcggctc atgatcacgc catcgaccca atcctcgccg tcgaagtact
      481 ggcgcgcgag ccgcgcctcc tcgacaaaac cggccttgac gaacgcgcgc aggctggggg
      541 cgttgctggc atagatgccg gcgagcagct tgcgcaggcc gagctcgtcg aacgccaccg
      601 tgcatgcggc ggcgatggcg cgctgcgcga tgccctggcc ccacaggtcc ttgcggccga
      661 tgatcagcgc aacgcgggcg tggcggtggc ggcgatcgat gctcgacagc cggatggtgc
      721 cgacatgtcc ctcgccctcg accatgatcc gccacagccg cgcggccggg ctgcgcgccg
      781 ccgccgcgac gtaagcgcgc tgcgagtcga gggtgtgcga atcgaaacgc gcctcggtga
      841 aacggacgac gtccgggtcg ttgagccagg cgacgctcgt cgccgtaacg tgttccggaa
      901 agtagggcgc gagccgcacc gcgccgatct cgctctcccg cacccggttg cccgccgacc
      961 cggacatgat cgagtccgcg gtcatcacgc ggagcgcgcc gcgaccgcag tcggacgctc
     1021 ccagttttcc ggtagcaagg gatcgctcgg gaacgcctcg atgccgcgcc ccgtcagcgc
     1081 ccaggtccgc aggtgctccg cgatcgccgg gtcctgcaga gccagccgca cgacctcggc
     1141 cggctcgacc tccgggcggc agcgccgata gccgaacgcc gccatctgtt cgccggtcac
     1201 ccattcgcac agcgctatct cggcatcggt gaggttgccg cgccagcgat cgatcgaggc
     1261 cgcgacatcg aaatcggcgc cgtcggcgcc gaacgccgaa ttcgcgcgcc acgacctgcc
     1321 ataggcgtcc cgccagcgtg cgcgcgagaa gtcggcgcgc gcggggtcga gcccgagaaa
     1381 ttccagcagg ccggcggcgg ccgcttcggg cgccgccgcc aaatcctcgt agcggatcat
     1441 gcgaaagcgg tcctcggcca gccggccggc gtagtcgagc gcggccttca ttgcatcgta
     1501 ggcattgaac acagcttcca gatagcgctt gcccggcgcg ctggtgtagc gcttgaacga
     1561 ggccagtacg ctgcgcggat cccgcaccac caggatcgcg cgcccgttcg gcatcatcga
     1621 taggaacgcg ggtatctcgc gccacaagag ctggcatttc tctgcccaat gccggcgccg
     1681 ccccgcgagc cacagctgag tcattacgac atcgtagagc acgccgtagg ttaccggcgc
     1741 gcgcgccagg caggcgtcga aagccctatc ggtgtcgagc tcgatgcgat agcggtcggc
     1801 aagccgaacg cccaggtcgg cgagcgcgca ccgcaggcgc ccggcctgag cgatcgggtc
     1861 gtagcggccg acgcagaagc gcagcacatt gacgtgatac atcgtgctgc tgagctcggg
     1921 atggctcgac agcaacaggc tcaacagctc cgtgccggat cgataggtgc cggtgatcag
     1981 ggtctggctg agcgacgccg ctaccggcgc cccgttgcgg ctgcgagtca tgacactctc
     2041 aggcccatgg cgtcgaggcc gcgccggccc gtggccttga gcgcatcggt gagccgcacg
     2101 cgccgatgcg tggcctggcc gcgaaacgcc tcaaggtggt cgatgctgtt gatcgcggtc
     2161 ggatcgtcga gcgcgtagcg aaggtcccag gcaaggaacc ggttgttcgc ggtcatcata
     2221 accgcggtga tctcgccgtc ctcgaccagc tcgtcgagcg gaacgatggt cgcatgacgc
     2281 ggcgcgaggc gccggcgcat cgtccgcccc gcggccgtcc tcattcgcac cacgatgtcc
     2341 gcgctgtagg gattgatcat caggtaggcg ttgccgctgt cgcgccgccg gtcgaccagg
     2401 gccgcggcgt gcatcaggca gaaccggttg atgccgctga tctgctcgat gatgatcggc
     2461 cgggcatatt tgtaatcagg gatcaggttg aggctcccgt gcgcgccgat cagcgtcgcg
     2521 tagccgacgc ttacgtcgat cctgcggaac accggcgcgt cgcacgatag tgccgcctcc
     2581 aggtagccga gccggtcgtg gctgatcctg cgccagacat cgcgcgggtc cgcgcggccg
     2641 ccggccacct gacacgtcac ccggtcctcg ctccggcgct cgccgtcgac atgaagcgtg
     2701 aagtcgatcc tgagcgggcc gtcgtacggg caatccatcc ggctatcgag gatcttctgg
     2761 atcggcaggc cgatctcgag cgcgccatag gtcgcgggca gcaccggcca gatcaggggc
     2821 gtgacgtagt gaaagcggcg ctcggtcatg gtcagttggg gaagtggtcg acgttccagg
     2881 cgccgttcgc ctggtagccg atgatgtgcc tgcgggtggg ctgcgtgctg gtgacctcca
     2941 aggtcagcgc acgatcgcac ggcatgtcgg tgaacagctg gtcgaggtcg atgatcgtgc
     3001 tggcatggcc gtccagcgac agctcggcct cggcggcgtg ggcgccgccc gggtcgctca
     3061 gcttcaggca caccgtggtc cgcgcgcgcg cggcgttgat caacgacacc gccgccgcca
     3121 gtcgcttgcc gttgccggcg atcgccaccc gcgtgcggtg atggagcgtg ctcgccatcg
     3181 cctgcggctg ggtgtggtac gccgtggtcc aggtcggccc ttcgagcacg acataggggc
     3241 ggatcgagcc gaacacgcct cggctgcgcg ggcgcagccg aatcaggaac cagccgcgcg
     3301 gcgggcaacc tggcatggtc gcaagctcgt aggagagctc ggcgccggcg ccgaggaatc
     3361 cacttctgca tgcgcgcgcc cggcccgtct cgtcgaacag ccgcagcgag tagtcgtaga
     3421 ccggcaccgg ccagtcggcc tcgaacagca gcgggtgatt gacgatgcgc aggacggtct
     3481 tgatccccgc gtcgcacacc aggaagcccg agtccgtcca gtcggtcggc cgcaccagca
     3541 agccggcctg cgttgccgcc gccggcccgg agaccgttgc ggggccgggc gtgcgcgcct
     3601 gatgtatgcg gtccagcacc acgtccagat cgagcaggca tgcggtgcat ctggtgccga
     3661 tgccggtgcg ctcgaccaag gtgtcgtagt cgccgccgcg ggcgaaatgc gcgcgaaccg
     3721 cggcctcggt cacgtcgaag cagaagcaca acgtgcgtgc gccggccgcg cgcgccgagc
     3781 gttcgctgcc ggtcaatgcc gcgccctcag tcacgtgcgg tgagcttcag catccggttg
     3841 tgccgcgccc ggtgctgcgg ctggagatcg tcctgccagt cgtccatcac ctccagggcg
     3901 aagccggcgg atcgcaccat cgccgccatc acccgatccg gataacagaa gtccttcacg
     3961 ttccgctcgt ggacggcgtc gtccggcgag gacgcatagt tgaacagcat gcgcccgccc
     4021 ggcaccagca cgcgccggca agcggcgaga agcatatgcg cttccgccct cggcatgtgc
     4081 gtaaacaccg attgcgccca gatgacatcg acacaggcgg cgtcaagctc tggcatcgcg
     4141 ttgtcgatgc tgagcacgaa ccgggggtcg cggtcgcaca ggccctggcg cgcgacgagc
     4201 tcgcgcgcaa ggcgcaggcg ctggcgcgag agctcggtgc cgatgtagtg gccaggctcg
     4261 aggtatggca gcaccgccac cgccgtgcgc ccgtagccgc aaccgatgtc gagcaagcga
     4321 tgctcggggc gcagcccgtg gcgctttagg tactcggcgt gatagcggcc acggctcagg
     4381 tcatacagac ccttgtccgc acccgggtgg ctgcgcccgc ggccgatgat cgaatcgatg
     4441 gagttggcgc gctcctggcg gtccagcatc caggcataga agtcgttcca ggatcgcccc
     4501 gccgcccgga acagcaggcg caacgccggc agcgcgcgcc aggcgaggcc tccctgcccc
     4561 cgcaaccgca ccgccgttcg ccgagccatg attggcctac agcttgccgc cgcgggccag
     4621 gtagtcgcgc tcggccaagc aggtcgcgac cagcaggtcc accgcgcgct ccgcctgagc
     4681 gggcgcgagc ggggtttggc cgccaaacgc ccgggtgccg ctggcatcga aggcgagctc
     4741 ggcataggca cggcgcaggc gcgccgccga ctggcggatg gcgagcgcct ccgcgtcgtc
     4801 gacctcgcgc aacgcttccg agaccaccgc cggcaggtcg gcaaagctcg tcacgcgcgc
     4861 gaccgccggc agaaagccgt attcgacatc ggtcgcgaac acgatcgccc gcttgccgag
     4921 cagcagcgcc tcgaacgctg ccgtccccgc gatcgtggcc acggcgcgcg cgcgcgccgc
     4981 gagctcgaca ccggggatgt tcgggtcggc catggccacg ttggggagct tgagcaggtc
     5041 ctgatagaat tggcgccggc ggtcgccgag gcgcgaatgc tccttgatca ccagccggaa
     5101 gccggccggc agcgacagcg cgagctgctc ggccatcgcc tggacgttgc tgaaatggcg
     5161 ggcgagcgac tggactgtgt actcgggctc cttcggcagc ggaaagaaca ggaacggcac
     5221 cgctgcgagg cggtcgaggt cgccgtcagc gctgcgcgcc atccagcgag cgagccgcgc
     5281 cgaagacagc accgccccgg ccgcccggtg gaacggctgc atcgccggca gccggccgtc
     5341 atgccggagc ccgcgcgcgt acttgaccgc ataggtgtgc gccagcacca gcaactcctt
     5401 ggcgacgccg cgcgcggtgt aacgccattg gaaccacgag aacgtgcgct ggctgctggt
     5461 cggcggccgg acctcatcga gcggcaccag cggttggtcg gcgaggcgct ggtaggtgcg
     5521 cgcgagcagg tgcgggttgc tgtacggccc gaacgaccac atcacgtcgc tccgctggcg
     5581 cgcatgccgc ggcatggtga ccggcaggcc gatcgccaac gccagcacga tcgaggtggt
     5641 gttgagcaag ttgccgggcc ggccgatcac caggtcgacg cggtgcgcct ggtagagctg
     5701 ctggaagaga cggaagtagc cgtttatgcg ccgatagatg tcggccagcg ccggcggccg
     5761 cgagttggcc agcggacccg cggcataggc caggtaggca ccgtaaatgg cgcgatcctg
     5821 ctggacgatg tcgcgcaggt agaccacgcc gtgcaccgcc tccaggcgcc gcgcctcggc
     5881 gaacaccgag tccgcgtccg cgggcccggc ggcggcgagc gcctgccgct cgatttcgcc
     5941 gagatcgaag atgcgatctt tgtccgtcgc ccactgcgcg cacatgtcgc gctggcgcgg
     6001 gccgccgacc agcaccagaa agcgtgtgcc gtagcggcgg cgcatgatcg tcatcaccgc
     6061 ctcgtcgagc cagtcgtaga gcgacagcgg cgacaggaac acgcagcgcg gcgcggcggc
     6121 ggcgacctca gtccccgcgg cccacccgag cgcctcgacg acggtggtca tggcacgtcc
     6181 gcgtgcgccg gggcgtagcc gaagaagttc aggaaccgcc cgaggttgcg acaggcgaag
     6241 tcgatttcgc cggcgctcag gcggcggacg ccgcgggttg cgaccgagtc ctggatatcg
     6301 agcaccgcct cgaagctcga attgctggga atgtcgcggc cgagcaccga cggccgcgtc
     6361 agcgacggct ggaagtcgag gccgcaccag gcggcgatgc ggcgcatggt cgcctcgggc
     6421 cgggcggtga ggtcctcgaa tcgcagccag gtgacacggt cgggatcggc gcggtgcagg
     6481 cgttctgcga gcgcattcat ctcggccacg cactgggcga gcaccggcca ggtgagcagc
     6541 ttctccctga gcccccgcat ctctctcacc cgcttgaggc tgtcgagggc gcggatcggc
     6601 tcgcgcacca cgacgatcgc cttcgcttcc gcgatgtggc tagtcgcggc catcgccgac
     6661 ttgccgtagt ccggcgcctt catggagcag tagcgcgccg gcggccggcc cagcgccgcc
     6721 gcgtaagccc tggcgagccc gtaccaaagc tcgcccacgc tcgctgcgcg cccgacatcg
     6781 agcgcggcga ggaaacggtc ctcgctccag tccagtgtcg ccgtctcctt ggcgatcgcg
     6841 gggttggtgg tgccgagccg caccggccgg tgcagcggcg gcatcacgtc cttggcacgc
     6901 aggccggcga cgaactccgc agccggcaag cggcccgcgt cgatgaaggc ggcgacgccg
     6961 ccgtcgggaa ggtcgtggag gtactcgaac aggtaccctt cggtcaccag cgaaaagatg
     7021 tccgggtggc cgtcgaacag gctgtagacc agggtcgtgc ccgaccgcgg cggcccgctg
     7081 atcagcaacg gccggaacgg ctgtcctgca tcgccgcgtc gaccggaccc ggtcatgggt
     7141 ctaccatcgc gtcgcgcgcc gacaggtcga gcatggcgcc gtaccactgg gtgtcccgga
     7201 tcaattccgc cggcgagccc tgctccagca ccctgccgtc ggacaggacg atcacgtgat
     7261 cggcgttgcg caccgtgctc atgcggtggg cgacgacgac gatggtcatg tgcgcgttgg
     7321 cgtgcatgtt ctcgagcgcg gcgcggatct tgcgctcgga ctcgtagtcg agttcgctgg
     7381 tcggctcgtc gaggatcagg atgctggccc gcgacaggaa cgcgcgcgcc agcaccaggc
     7441 gctggcgctg cccgccggac agccggctgc cggcctcgcc gaccaaggtg tcgtagcgct
     7501 cggggagctc gacgatgaag tcgtgggcgt aagccaggcg gcaggcctcc tcgacctccg
     7561 cgtccgacgc gctcgggcgc gcgtagcgca cattgtccgc gaccgaggcg ttgaacagga
     7621 agttctgctg cggcgcgaac gagatgccgc gacgcaggct cgacagcgag aacgagttga
     7681 gcggcacgcc gtcgatgctg atctcgccgc gatcgggcac gatcagcctg gggatcatgt
     7741 cggccagggt cgatttgcct gcccccgacg ggcccatcaa ggcagttgtc acgcccgccg
     7801 ggatgcgcac gctgacatcg cgcagcgcgg cctggcgccc agaggggtag gagaaccaca
     7861 cgccgcggaa ctcgatcgcc cgttccaggc cccggaacgg gatcgtgccg cggtcgggct
     7921 ccttgtgggc gcgcgcctgc gaaacgacct cgacgatctc ctcgagcccg gcgtgatgcg
     7981 tgatgagcgc ctggcggtga ttggtgaggc cctgcgcgat cggaaccatc ctcaggatca
     8041 tcaggatgaa cagggtgatg atcgacaggc tgaggtcgag atattcgacg ccgacataca
     8101 gcgccgacag agcgaacgcg gtcatcgccg gcgcgatcac cagctgcatg cgcccggcca
     8161 gccgattgag gtcgaccgac aggcggaaga actgctgggc ccagtcgccg aagctccggg
     8221 cctcgcgatc gagggaatcc gacaccttga tcacctgcca gccctggtgg cgctcggcca
     8281 tgaaggcgca gaaccgttgg cgcagcttga ccacctcgcg gctgacccgg cgcgacaggt
     8341 gcatgtaacg gccgaggctc atcaccaccg ccgccgcgac cagcagcgcg agcagactcg
     8401 cccccggcgc cgtggcaacc atgaccccgc cgtaagcgat gaacgacagc atcatctgcc
     8461 agatcgccgc gtagctgcgg accagcgacg ccacgtgctg gcactggtgg tcgaggatgt
     8521 tgacgaaacg tccggcgttc atctccttca tgaacagcgc attgctgccc atcaccgccg
     8581 agaagcacag cctggacagg tcgcggccga cccgctgctt gatgcgcgtg acctgcatga
     8641 catgagcgta gttggcaacc tggcggcaca gcaccagggc cacgatcacc gccgccagcg
     8701 agcccagagt caccggcatg tggacggcgt ccgccacctt gccgatcacc acccacagcc
     8761 gcgagcgttc cgccagcacg gcgaggtcgc gcccgccctc gatgtagtcg agcaacggat
     8821 agatcatggc gacgctgaac atctcgaagg caagcgcgag gatgccggtg gtggcaatga
     8881 cgcccgcgtc cgtgagcccg accaatcggt agccgcgcag gatgcgtatc tcctcgcgca
     8941 gctccgcggg caggatgccg gtgatcaggt gcagcgcgcg tgcaagcatt caggaagcgg
     9001 cggtgacggg ctgggcgagc aggtactcgg ccatgcgcca gtcgagctcg gtgtcgatgt
     9061 ccaacgacgc ccgcgcgtcc atcacgaccg gcagcgtgcg ctccggaaac agggatcgcc
     9121 gacgacggac gatctcggtc cggatcacgt agagcgcccc attcggcgtg tagagcggcg
     9181 ccgagtcggc ccccgccggc gcgagaaagc catccgcgcc gagccggaac acggtttgcg
     9241 gggtgcgttc gagagcgcgc acgccgacca cggcgtctgc gtccgcgcgc gcctcgagca
     9301 gcgccaccgc ctcggcaagc gtcgccgccg gcctcagcgg cgaggtgacc tggagcagca
     9361 tcacgagctc cggcgcatag ccggcgctgc ggccgaatgc atcgagcgcg tgcagcagca
     9421 tgtcgaccga ggccgagtcg tcgcccgata gatgtctcgg cctgacgaac ggcaccagcc
     9481 agccgaggcg ccggccctcg gccgcgatcg ccgcatcgtc ggtgctcaac acgaccgggg
     9541 catcgagcgc cgcctcgccg atcgcggtgt cggcatgggc gagcaggtta cgcccgcaca
     9601 gcggccgcaa gtgcttgccc ggcaggcgct tcgagccgcc gcgcgccggc acgacgatca
     9661 gcagcgtggg ccgcgccacc ggctcacgac accatgtggc gcagccgcca gtcgctgccg
     9721 acctttttcc acaggtgcgg cgagcggaag cggtcggcga cggcgaagaa cgtcgtctcg
     9781 tccatgtcca ggtagtcgag gaactcggcg aagtacttgc gcgggaactc gaggtcgtag
     9841 cggtgcacga gcgccacccc ttcctcgcgc gtgatgtgct ggttgcggat ctcctgggcg
     9901 gcatcgtagg tcgcccggcc gatgccgaac ttgaccagag tcgtccagta gtggaacggg
     9961 tcggtcttgt cgtcgatgct gttgtacttg gagtaggtgc cctcagtgcg ctcgtcgttg
    10021 gtgcaaaacc cggtgtgctc gaccgcgtag tagtagttct gctgcggcgt ccagctcagg
    10081 tagtagccga ggtagtgcac ctcgatctgc gccgcgtcga gcgcattcag gtcggccggc
    10141 agatagggct cgatccgtcc cggcgccacg ccgtggcgcg gcagctcgtc gatcggcacc
    10201 ccgcccaagt aaatgtcccg gttccacatg cgccggcggg cgaagtagtc ggcgccgcgc
    10261 aacgccgagg cgttctcgtc gatcgggttg ccgtactcgg cctcgttctc gccgtagaag
    10321 atgagcggga cgccgaagcg cagcgccatc ttgggcgcga agttcttctg acccaggatg
    10381 aacggctgga acgggtgcag caggttgcgg aacgcgagcc gggtgagcgt cctgtgcact
    10441 tggccgtcgg gagtgaacag gtagttgtcg aggccgccgc gatggatcca gccctggaag
    10501 ttgcgccagc cgatgtcggt gtagagatgc ggcgcccagg tcacggtcag cgggtgcatg
    10561 ccgtagctgg tcttgagtac gtgcgaggcg tagaccgagt ccttgcctcc cgatcccggc
    10621 accaggcagt cgtacgagcc gttctgcgag cggaagcggt cgcacagctc gcgcaacgcg
    10681 cgcgcgcgct cgtcccagtc gatcgcggtg cgcttcgtct cggcgtaacg gcaggcgtcg
    10741 caaacgccgt ccgcgtcgaa ccggatggtc gtcttcttcg aatcggggcg atgcgcgaac
    10801 tcgatcgtcg acgacggccg ctggttcgag atcacgcagg tccggcagaa ctcgaccttt
    10861 ggcggcagcc cgtacttcac ctccaggcgg ccgtcctcgg ggtcgatcac gaagctctcc
    10921 tcgccgcggg taatcgtcgt catcctgtgc ctgctattcc ttgccggggc acgcgatcgc
    10981 caggtagtgc cagtcgagca cgagcgcgcc gttgatctgc atgtcgccga ccaccttggt
    11041 ggcgggaatg ccgtcgaact cggcgagcgc ggtgttgtcg aacggcgcga agtcggtatc
    11101 gcgctcgaag cggtcgatgc gcccgccgag atgacggacc aggccctcga ggtagggccg
    11161 gctatagatc atgtagagtg agaaatcgaa gtcctcgcgc tccggcgtga tcacgcccac
    11221 ccggtcgaag tcctgatcgt agatcacctt gaccgcgtag ttgcacggcc cgatcggctg
    11281 gcgccagacc acccacttgc gcgccacccg catcgcctcg ccgagcgccg catcgatgcg
    11341 cgggaagaag tggaacaggt tgacggccac gatgtcgtag gcgtcgtcga cgaacggcat
    11401 gtcgagcacg tcggcgtgca cgaaggcggt gcgcgcgccg gtgccgaaga tctcggcgcc
    11461 ccaggcgagg tagcgggcgg tgatgtcgag gccggtgtag tcgacctcgt cgtcgagcag
    11521 gcggcggaag ctgcgcacga aatggccggt cgcggcgccg aggtcgagga tgctggcacc
    11581 gggccggtag atgccggtgc gcgccaggaa cgaggcgaag ctcttcgacg cctccttttc
    11641 cggaagttcg cccttcgcgc gcttgtagta gctgtcgccc atcttctgga cctgatcgag
    11701 gtcccatggg ttccagggtt gcgaatcggc gccgcccctc agcaggctgg gcacgccgcc
    11761 gttgaccggg aacgacgccc cgcaggcggc gcagcgcagc cgctcgccgc gcgcctcgct
    11821 cagcagcccg ccggcgtggc agtcgaggca gcgcaggcgc gcaagcgtcg cttcggcgac
    11881 ggcggcgccc gcgctcgtcc gaagcccgct catgaacggt cgagcgccac gaagttgcgg
    11941 atcacggcga gcccggtctc gccgctcttc tcgggatgga actggcaccc gatcaagcct
    12001 cccttgcgca ccatcgccgc gacctggacg ccgttgacgg caatcgtcgc cactacgtcg
    12061 cctgcatcgg ccggcgcatg cgcataggag tggacgaaat acatcatcgc cccctcggcc
    12121 aggcctgcgg cgatcgggtc ggggcgacgg acctcaagcg cgcgccagcc gacattggga
    12181 atgcggatcg cgcccggccc gttgccggcc ggcaggcgac ggaccgctcc cgccaccagg
    12241 ccaagcccgt cgtggcggcc gaactcttcg ctctcatcgg cgaacagctg catgccgaga
    12301 cagatgccga gcatcggcgt gccgcgcgcg gccgcctgcc tgagcgccgc gaccaggccg
    12361 cgcgcctgga gcgcgcgcat ggcgtccgcg aacgcaccga cgcccggcag cacgatgcgc
    12421 tcggcggcga gcacggcctc ggcgtcgtcg gtgatttcga cctcgcccgc cccgcaatgg
    12481 cgcagcgcct gcgcgatcga cagcaggttg ccgcggccga agtcgaccac cgcgatcacc
    12541 tgcgcacctc gatgccggcc gaggacaacc gctccttgac ctcgccgacc ggacagctcc
    12601 ggtcatggaa gatcgacgcg cagcacaccg cgtcgcacgg cgcgcgcgcg agtagtgcgg
    12661 cgatgtcgtc ggcgccgccg gcgccgccgc aggcgatcac cggcacccgc acgcggccgc
    12721 agatctcggt gagcaggtcg acgtcgaatc cgccgcgggt gccctcggca tcgaccgagg
    12781 tgaccaggat ctcgccggcg ccgagcccct cgccctcgac cacccagtcg aggacgtcgc
    12841 gcccggtgcg ctcgcggccg ttgtcgacca gcgcctccca ggtcccttcg gcgcgccgct
    12901 tcgcctccac cgacagcacc acgcattggc tgccgaaggt ctccgccgcc tcggtgatga
    12961 actgcggccg gcggacggcg gcggtgttga tcgccacctt gtcggcgcca ctgcgcagcg
    13021 ccgccttgat gtcgtcgatg ccgcgcagcc cgccgccgac ggtgagcggg atgaacacgt
    13081 cgtgcgcggc ctgctcgacc accgacagga tgttgttgcg ctggtagagg gaggcgacga
    13141 cgtccatgta gatgatctcg tcggcgccct cgacgtagta gcggcgcgcc atctcgcccg
    13201 gatcgcccac caccttcagc ccttccaggt gcacgccctt gatgacgttg ggccccttga
    13261 cgtcgagccg cgcgacgatg cggatcgggg tcacgccgcg gccgcgtcca gcaccgcgac
    13321 cggccgcgcg cgatcgctct ggaactcgag ccggcggatg caggtccggc cgaagcggtc
    13381 gttcgccgcc acgccgacgc gcaggacgtc ctcgattggc aatgtgtcgg cgtggaacgc
    13441 gtaatgccag cgccgccggc acggcgagaa gtcgaagttg tcgtggatga accgccgcga
    13501 ccgggtctcc agcgcaagga acggctgcgg gccgaacacc tcgccggcgg cggtgcgcac
    13561 ctcgatgcgg gcgacgtcgc tgcggtcggc ggggtggaag gcgagatcga gcgccagcgg
    13621 ctcggcgccg tcggccggcg cgccgatcat cctgcggaag ccgtcgctgg cgcggacgta
    13681 ctcgaacgcc accgtcggga accgccgccg cgactcggcg atcatcgcgc gcacatggtc
    13741 gatctcgggc cccaggtcac ggaagtcgtg actggcaacg ccgaccaggg ccgggcgtcc
    13801 ggcgtcggcg cgcgcgaacg ccatgtccat ctcgccctgg tcgaggttgc cgatgcgatt
    13861 catcacgttg agcgcgcgcc cgatccagcg ccgacagccg ccgggtcgct gatagtcgtc
    13921 atggctcggg tggtagatgc cccagtccgc gggcgccagc cgccagtcgc cggagcggcc
    13981 gttgcggaag tcgatggtgt cgtcgagcgc gcggttgtcg tcgagcgcca tgctcgagat
    14041 gtcgaacggg atccactgct cgaggaacca gtggctgtcc ggccgctcgg cctggaagcc
    14101 ggcgcggaag gtgctgggga accacgcccg ctcgatcacc ttgcggcaca gcacctggaa
    14161 gacgtggtcg gtgcggaagt agtgcgtcgc gcagcgatgg gcatcgccat aagtggacat
    14221 cgggtggaaa tgccattgca gctcgtcggg acatcccggc gcagcagcca ggcggcgggc
    14281 gtaatggtcg aacacgttgt ggaagccgat gtcgcgccgg cgcgggttgt agtcgtagcc
    14341 gacatggtcg aggcagaacc aggtgaacac ccagccgttg cccgccgagt ccggcagccg
    14401 gtcgcggaac gcgggcgcgg tgacccgctc cagcatcgcg tcgacctggt gccacgattc
    14461 gagataggcg ttgcggtgcg acgacagggt cagcgccacc gcctcctcga tgccgccgag
    14521 cgcgatctcc ttgtcgcgca gcttgcgcaa ggtctcggcg gtgcgcggca gatcgtcgat
    14581 ggcgaacagt tcgctcaggc gctcgaacct ggcgtcgagc gactcataga gcggcccctc
    14641 ggtgtcgatg cagtgcgcga tgtagacctt cgcggtcatg cgcgcagcgc ggcctcgatc
    14701 tcttcgggcg cgggcgcgcc gggcgcggca aagcgcttgg cgagcaacgt cgcgcgctcg
    14761 ggcagtccgg cgaccagcgc cgcgatgcgc ggcgcgctga agccgtcgcc gtacgggctc
    14821 gcccccggcc ggcgcggcat ggcagcggca cgggcaaggg cggtgcgcac ggccgcggcc
    14881 tcggcggcgc agtcgatgac gttggcgccg cgctcgcgtc cctcctggcg cccgccgaca
    14941 ttgaccaccg gcaagccgaa cagcggcgcc tcgatcaacc cgctcgagga gttgccgacc
    15001 atcactgcgg cctggcgaag caggttggca tagagcgtgg tccccgcagt ggcgaggtac
    15061 tgcgcccagc cgcgggcggc gacgaaatcc gccacccgct cggcgatggc gctgccgccg
    15121 gggtcggagt tggcggcggt gaacagggtc ggcgcgggat cggcgtcgag cgccgcgagc
    15181 accgcgtcca gcggcgcgag cgggtccgag gagttggtct cgctgtgcac ggtcaccaac
    15241 cgcaagccgg cgaccgttgg cagatcgagc tcggcggcaa gcgcatcggc ggcgaggcgc
    15301 ggtgccgccc gcagccggtc gagccccggc gcacccgtga ggtggatgcg ccacaattcc
    15361 tcgcccatct gggcaaggcg ccgggccgcc gccgcggtgg cgacgaaatg gagatgggcg
    15421 agcttggtca gcgcatggcg gatgcgatcg tcgaccgctc cggcgctgat ctcgccgccg
    15481 tgcaggtgcg cgaccggcag gttgaacggc agcgtcgccg ccgccgccgg cagcatgtcg
    15541 agacggtcgc cggtgaccag cacgatatcg ggtcggatgc gccggtagac ctcgcccgcg
    15601 aaggccgcga tcgcggccat cgccgccgcc gccgcgggct ccgtgccgcc gaggtcggcg
    15661 ccgccgcggt gggtggtggc ggcggccggc gtctcggcct ccgcggcatc gtcgcggcag
    15721 tgcatgccgg tcaagaggac gtgcaggtcg gtgcccgcgg tctcgacgag ggcgcgccac
    15781 accgccgact gaatgccgga atcggcgcgg ctgctcgaca cggcggcgat gcgcacggtc
    15841 acgtcggata ccgggtcaag gcggggacca ggcttgccgc gggcacccgc gcgccaggca
    15901 gcaccaccgc gccagcgccg atcatggcgc aggcgcccac ctgcgcgccg ccgaggaccg
    15961 tcgcccgcgg cgcgacatgg gcgccgtccg cgatctcggc gtcgtgctcc accaccgcgc
    16021 cggtattgac aatcaccgcc atgcccaggc gtgccccgct ctgaatgacc gccccggcca
    16081 tgaccacggt gcccgcgccg agcaccgcat cggcgctcac cgtcgccgcc ggatggatca
    16141 ccggcgcaag cccggcgcgc gccgcaacga ggcgatggac gatcgcgagc cggctttcga
    16201 ggtcctccgg gcgcaccccg ccgagaccca ccgccacggg atggcccgcg gcggtggcga
    16261 gcgcctggtc gtcgtcgagc tgcaccgcgt cgagccacgc cgagggccgc gggtcggcat
    16321 aggcgctgat cctgtggccg gcggcgcgca gcgcgtcggc caccacccgg ccgtggccgc
    16381 cggcgccgat cagcaggaca tccatgcccg ctcgaccgcc gcgctgccct gccatgcggc
    16441 gaccgcgtcg atgacccggt cctgctgggc gggtgtcagg ccggtgctgc acggcagcgc
    16501 gacgacgcgc tcgccgatca accgcgagac gcccgtcagc cggcgcggcg cgtcccgata
    16561 ggccggctgc gacgacaggg cctgccagaa cacccgcgcc tggatgcgcc gcgactcgag
    16621 gtagcggatc aggtcggccg cgtcgcgcgc gcagccgcaa ctcaccgagt acatccagca
    16681 gctgctgtcg gcccaggccg cgcgcggcat cggcaccagg tccgtgcggg cggcgaaggc
    16741 gcggtcgtag gcggcggcga tgctccgctt gcgcgccagc atctcgtcga gccgctcgag
    16801 ctgagcgagc ccgaccgcgg cgttgatgtt ggtcatccgg taattgaacc cgaccaccgc
    16861 atagcgatag aactccgtcg gcgtccggcc cgcgtcggtg aggcggtgga cactgagcat
    16921 gcgcatggtt tccgcaagct gcgagtcgct ggtcagcgcg gcgccgccgc cgccggcggt
    16981 gaccgtcttg ttgccgttga acgagaacac gccgatgtcg ccgaggccgc ccgccgagtg
    17041 gccgcgatag cgcgcgccga tcgcgccggc cgcgtcctcg accagcggca cgccgtgcgc
    17101 gcggcaggcg gcgctgatcg gatccatgtc ggcgggatgg ccgagggcat cgaccgcgat
    17161 cacggcggcg aactcctgct cggccaacag ctcgttgacc aggcctgggt cgagcgtcca
    17221 gctgtcggcg ctgacatcga ccagcagcgg gcgcgcgccg gtgtaataga ccgcgttggc
    17281 ggtggcgccg aaggtccagt cgggtacgat caccgtgtcg cccgccttga ccccgagggc
    17341 gagcagcgcg agatgcaggg cggcggtgcc gctggaggtg gcgaccgcgc gctcgcagcc
    17401 ggcgagcgcg ggcagccgcg cctcgatctc gtcgacgaac gggcccgcgg tggacaccca
    17461 gttgtcgtca acgcaggaca gcaggtactc cttctcgcgc ccgcgcaggt ccggcacgct
    17521 cagcgggatg tcgtacttcc gttccatcat tcgcgctcgc ggttggcgac gcgatcgaga
    17581 tcgcgctcgg cggtttcgag gtcttgcggc cggccgaggt ccttccagta ctcgtggatc
    17641 gggaacaggc cgaccctgag cccggcgccg cgaccgagtt ccatcagatc gggcatgtcg
    17701 aggcgctggt cggggcggac cagagcgcag aactccggcg acaaaagata aattcccgca
    17761 gccacgaaat agtgcatgca cggcttctcc tggatgccaa ggaacaggcc gtcgtcgttg
    17821 tggcggacca cgccgtaggg cacgtcgacc tgatactgcg cgaccgccac cgtgccgtcg
    17881 tgccggttgc ggcggtggaa gctgaccagc gcgccgtagt cgacatcggt cagcacgtcg
    17941 ccgttgagca ccagcagccc gccggcgggc agatcgtcga tccgcccgat cgcgccggcg
    18001 gtgcccagcg gctcgtcctc gcgcaggatc gacagctcgg cgatacccgg ccgcccggcc
    18061 gcgaattcgg cgatcttgtc ggcgaggtaa tggacggcga tgtagacgcg ccggacctgc
    18121 gtctcctcga ggcgcgcgat gagccgctcg acgatcggct tgccgcccac cggcagcagc
    18181 ggcttgggca ggtcgcgggt gcgctcgccg aggcgcctgc cgagcccacc ggccatgatc
    18241 agggccgccg gcggctcgct ttcgacgtcg gcgaaggcga gaatctcgcg caacgtgccg
    18301 gactcgtcga ccaccggcag aaaggccgcg tgcggactgg cgcggcgcag ccactcgagc
    18361 aggtcgcggt tggcgtcgag ccggtcgatg cgcccaatga ccggctcgcg ctgcatgatc
    18421 cggacgatcg actcctcgac cgcgacgccg ctcaggatcg cgcgccgcac gtcgccgtcg
    18481 gtgaccgtgc cgagcacccg gccggtccgg tcggcgacca cctggaacag gttgccggtc
    18541 tcggtcatcc gctccatcgc gcgccggacc gtcgtgtcgg gcgcacagac gatggcggcg
    18601 aaatcggtga gacgaacgag gctcatgacg cgtgacgccg acgttgcgcc tcttcgatgc
    18661 cacgcgtcgc ggtctccggg ctcgccatgc ggccggcgcg cgcgccgaac gcgagacggc
    18721 gaatgatgcc gctgtcgtcc tcgagcgcgg gggcgggcgc cggccgcgct gcgccgccca
    18781 gcaccgccgc cagcgtcctg cgaaaggcat cggcgtcgcg gttgacgaag tgggtggtgt
    18841 aagccggatt caatgaccgg tgcccggtca gctcgaggaa cgcgtcggcg tagtctgaga
    18901 actcgatcgt cggcacgccc atgaccagcg catcggcctg ggtggtgctg tagtagttgg
    18961 tgacgaagca cgcggcgcgg gtggcgagca ccgagggatg cagatagctg accgcgaagc
    19021 gcgcgtcgga gaagcgcgcc agcgcgtgtt tgacgagcgc catgtcggtg accgcatggg
    19081 gcttgatgaa caccggccgg cttttcgcca cgtccgcgag cacggtcaac gtggcgtgca
    19141 gcagctcggt ccccgaccgc tcactcctga ggaagtcgag cggccccagg aagccgagca
    19201 tgtagaccac gatctgttcg ctcgccggca gctccaggcg gcggaactcg gcgtcgaacc
    19261 agcgccccgc gttgtcccgc
//
LOCUS       DASWHU010000408        15823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2540389, whole genome shotgun sequence.
ACCESSION   DASWHU010000408 DASWHU010000000
VERSION     DASWHU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540389"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VGB88_15000"
     CDS             complement(<1..1497)
                     /locus_tag="VGB88_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9648812.1"
                     /translation="MVARCHTVAFQGIDVVGVEVQVQIAAGLSAFTLVGLPDKAVAES
                     RERVRAALGALGLSLPAKRITVNLAPADLAKEGSHFDLPIALAILAAMAILPADALDG
                     FAALGELALDGRISPVPGVLPAAIAASAAGRGLICPAALGGEAAWAGEIEVLAAPDLV
                     ALVNHFKGSQVLTPPAPLMQAAAAGPLDLADIKGQETAKRALEVAAAGGHNLLMIGPP
                     GAGKSMLAARLGDLLPPLASSEALEVSMIASIAGALQGGRLLRRRPFRDPHFSASVAA
                     MVGGGTRARPGEVSLAHLGVLFLDEFPEYQRPVLEALRQPLETGTVTVARANAHYTYP
                     ARFQLVAAMNPCRCGHLDDAERACSRAPRCAADYQARISGPMFDRIDLHADVPALAAA
                     DLALPPPAEGSAQVGKRVAEARAVQSERYAGETRAGGAPVRTNSDADGALLERVAAPD
                     ERGRALLTDAVDRLRLSARGYHRVLRVARTLADLDGANGVTRRHVAEAL"
     gene            1846..3087
                     /locus_tag="VGB88_15005"
     CDS             1846..3087
                     /locus_tag="VGB88_15005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9648813.1"
                     /translation="MVKGLLALAAIIAGTMVLQLANAILGIIVPLQMGLLGKSTAVIG
                     AVVTAYALGFLLGCLGAPAFIRPIGHIRAFAALAATLSVATLLFTTTDSPLAWAVLRF
                     AMGACVAAVFTVVESWITGEAPKGDRGRVIAVYMVCNKTGVMLGQGLLVVGDAASAGF
                     FVFVCACFSFSLVPVALTRAGGPVPQDAVTLGIRALYRIAPVGVVGCLGAGLVNASVL
                     GLAPVYGLGIGLAPERIALLVLVAQLGSLLLQWPLGWLSDRIDRRQVIVGACATGAAA
                     SLAIALWGRDDPLLLLGLFALWGAFSLSIYAVCIAHASDFAKPAELLPLISSLLVAWA
                     IGSTVGPPLATLAMTAAGEDGLFAYAALVTALIGAFAGWRMTRRRAPPRALREAFVNM
                     PATSPAVAELSPRVGRETDAE"
     gene            complement(3097..4047)
                     /gene="gshB"
                     /locus_tag="VGB88_15010"
     CDS             complement(3097..4047)
                     /gene="gshB"
                     /locus_tag="VGB88_15010"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX9648814.1"
                     /translation="MGLKVAIQMDPLAGIDIDADSTFALALEAQARGHALVHFLPRAL
                     SMVESKVVAEARALQVRRKKGDHFTLGPPETVALSTMDVVLMRQDPPFDMAYITATHL
                     LEHIHPQTLVVNDPAEVRNAPEKLFVTHFEGLMPPTLITSDRRQVADFRARHRDIIIK
                     PLFGNGGAGVFHVGPGDENLNALLELFTGLYREPIVVQKYLPEVRRGDKRIILVDGEP
                     VGALNRVPAAGEARANLHVGARAEPATLTAREREICAALGPELKARGLIFTGIDVIGG
                     WLTEINVTSPTGIQEIARFDGTNVAALIWDAIEARVAAGT"
     gene            complement(4075..4917)
                     /locus_tag="VGB88_15015"
     CDS             complement(4075..4917)
                     /locus_tag="VGB88_15015"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9648815.1"
                     /translation="MIAELPAPASAEEEALRGAVRAGEAGPGIAEQALLLAAIDRPET
                     DPAPYRAHLRALAAEARAGAAAETVEGAARALAASLFDGYGYDGDRDDYEHPDNANLM
                     RVIDRRRGIPVSLGILCLHWLRAGGRGAVGLAFPGHFLIRIEDSSGRAILDPFHRCRT
                     VGSGDLRDLLKATAGPEAELLPHHHAAVSDAEVLLRLENNVKLRAFQRGDVGRAIGAL
                     RAMLILAPDSGGLLRELAILSARRGSLRDAAWALARFLERSPPGAEREEASALLASLR
                     GRLN"
     gene            complement(4914..5525)
                     /locus_tag="VGB88_15020"
     CDS             complement(4914..5525)
                     /locus_tag="VGB88_15020"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9648816.1"
                     /translation="MRRRRAAALALLAALAAGPVLSSCSPLGVATGAGATAGVAVAQE
                     RPVGEAVSDTAIDLEINHALFMAHIDDLYREVNIDVVEGRVLLSGTVRGQELVDRAAE
                     LAWKVDGVRQVINEIRIGDDTAVDVVRDRWITTKLRGHLLTDRQVLDINYSITTVNGT
                     VYLLGIAQDQAELDRVIAHARTLEHVRRVVSHVVFKDDAARRG"
     gene            complement(5522..5926)
                     /locus_tag="VGB88_15025"
     CDS             complement(5522..5926)
                     /locus_tag="VGB88_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9648817.1"
                     /translation="MATPRTDRRAARHGALRFGRTAEAACRWRLRLCGWQILARDWRT
                     PVGEIDIIARRGPVIAFVEVKARADIDAETAPPAPRQRRRIVRAAELYLARNPRFGAL
                     CARFDVMLVRPWPSPRFLWPVHHPDAWRESAW"
     gene            complement(5907..6806)
                     /gene="rsmI"
                     /locus_tag="VGB88_15030"
     CDS             complement(5907..6806)
                     /gene="rsmI"
                     /locus_tag="VGB88_15030"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9648818.1"
                     /translation="MAKARSRGDGSAGHRPSKLDPGLYLVATPIGNLADITLRALAAL
                     AGADLVACEDTRHTGRLLAAHGVAAKLVSYNDHNAPRVRPRLIAQLHAGAIVCLVSDA
                     GTPLVSDPGYRLVTEAIAAGVPVHPVPGPSAVLAALTVSGLPTDRFYFAGYLPARAQA
                     RRAALARIAAIDATLVLLESPRRLGALLADAAAALGARPAAVARELTKLHEEVVRAPL
                     DALAGRFADRAPRGECVLVIGAPAEPAGQAVSDAEVDRLLGAALAELPPARAAQAVAQ
                     RTGRARRALYARALALRGGDAAD"
     gene            6986..8092
                     /locus_tag="VGB88_15035"
     CDS             6986..8092
                     /locus_tag="VGB88_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX9648819.1"
                     /translation="MVSPLAPPSAVPVAPPGRPLAAGEVRVGILLPFTGAHAALGEAL
                     LNAASLALFDHGGDTVTLLPKDTGSTGSGADSAVRAALGEGARVVVGPLLASSVRAAA
                     PAARQAAVPVLAFSNDSSVAGEGVFIMGVTPAQQVARVVALARAQGHARFAALAPDTP
                     FGNAVVSALFEAAEASGGAVDRVELFVPGQVSVSENEEVRRLAAAAKGGAFDALLIAA
                     GGADLRTMAPLLPYYGVDPDRIQLLGTELWHDPAVLGEPALLGGWFAAQPDDAFDQFR
                     GRYRSVYGAEPPRLAALAYDAVALVAALARSPRGPDFSRAVLTDADGFAGIDGIFRFG
                     DDGVNQRGLAVYRIAARTFEVVDPAPASFAAPAF"
     gene            complement(8020..9270)
                     /gene="hemW"
                     /locus_tag="VGB88_15040"
     CDS             complement(8020..9270)
                     /gene="hemW"
                     /locus_tag="VGB88_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX9648820.1"
                     /translation="MAAAERALSVYVHWPFCRSKCPYCDFNSHVAEGIDQGRWRRALL
                     AELDEAADRIGARAVQTVFLGGGTPSLMAPATVAALIDRIARRFDLAGDAEITLEANP
                     TSAEAARLADFAAAGINRISLGVQALDNAALRFLGRAHDAGEALAAVAAARAAVARVS
                     FDLIYARPGQTPAAWRAELMRALAHAQGHVSAYQLTIEKGTPFFAARRAGEFASPDED
                     TAVALWQVTQEVLEAAGLPAYEISNHARPGEECRHNLAYWRHRDYAGIGPGAHGRLAH
                     AGRVVATETIPAPAAWLAAVEARGHGRRRRAALTAAELVAEVLLMGLRLTDGVAEADL
                     RRRTGLAFAEALDARAVAALADARLIRLRPGRIATTPQGRLLLDAIVARLTPGRAGAA
                     DQNAGAANEAGAGSTTSKVRAAMR"
     gene            complement(9257..9886)
                     /gene="rdgB"
                     /locus_tag="VGB88_15045"
     CDS             complement(9257..9886)
                     /gene="rdgB"
                     /locus_tag="VGB88_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9648821.1"
                     /translation="MARRLDGPRVLVASHNPGKVAEIAALLAPFGIEAAAASGLGLAE
                     PAETGASFAANARIKAEAAARAAGLPALADDSGLEVAALAGAPGLHSARWAGPERDFA
                     RAMARIEAELGARRDAEARFACALALCWPDGHCELFEGTVEGRLAFPPRGGRGFGYDP
                     IFVAAGHRRTFAEMAPADKDRISHRARAFAKLAAACLAPAAEESSVGRR"
     gene            complement(9870..10616)
                     /gene="rph"
                     /locus_tag="VGB88_15050"
     CDS             complement(9870..10616)
                     /gene="rph"
                     /locus_tag="VGB88_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX9648822.1"
                     /translation="MRPSGRAPDQLREVVIETGVNKYAEGSCLVRFGDTQVLCAATVE
                     DRVPPFLRNTGRGWVTAEYGMLPRATDVRTDREAARGRQSGRTLEIQRLIGRSLRAVC
                     ALDGFGERQVRVDCDVLQADGGTRTAAITGGYVALHLAFDYLVRNALIERSPLTDRVA
                     AISCGLYQGIAVIDLDYAEDSTAEADANFVLTAKGGIVEVQATAEAAPFRAEHLDLLL
                     ALARAGTEQLVALQGEAIARALAARDGAPA"
     gene            10783..11865
                     /gene="hrcA"
                     /locus_tag="VGB88_15055"
     CDS             10783..11865
                     /gene="hrcA"
                     /locus_tag="VGB88_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9648823.1"
                     /translation="MSTTPKSAMFSELNERSRLIFSQIVESYFDTGEPVGSRTLSRKL
                     PMQLSPATIRNVMADLEDLGLLFAPHTSAGRLPTERGLRLFIDGLLEIGDLSAEERDN
                     IESQCAGAGRTLSEVLNQATEMLSGLTSAAGLVLAPKSEAPVRHLEFVGMSPGRALAI
                     MVLANGLVENRLIDLPVGITASALVEAANYINSRLIGRTLGEARGMIEVELAARRAEL
                     DALATRVVEAGLATLANVSDEPTLIVRGRANLFEDVSALADLERIRSLFDQLEQRKNL
                     LRLIDSTLGGPGVHVFIGSESKLFDLAGCSMIVAPLGAKRGLGDGSAAGACVGAIGVI
                     GPTRINYARIIPMVDYTARAIGRLMS"
     gene            11915..12499
                     /locus_tag="VGB88_15060"
     CDS             11915..12499
                     /locus_tag="VGB88_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9648824.1"
                     /translation="MAEPKPQSDAPEPQAPAEAEGVPAGETPPAAEELLRAELAELKD
                     QLLRALAEADNQRKRAARERDETAKFAIAGFARDVVQVADDLERALASAPRAGGDGQA
                     SIASLVEGVELTRRSLQAALDRHGVRRIEPLGERFDHNLHEAMFEVETADAEPGTVVQ
                     VVQHGYRIHDRLLRAARVAVAKPPAPGAGVDTEA"
     gene            12654..14585
                     /gene="dnaK"
                     /locus_tag="VGB88_15065"
     CDS             12654..14585
                     /gene="dnaK"
                     /locus_tag="VGB88_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9648825.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGKDAKVIENAEGMRTTPSIVAFTD
                     DEVLVGQPAKRQAVTNPENTLSAIKRLIGRRWDDPMVRKDAGMVPYKIVKADNGDAWV
                     EAGGKRHAPSQISALVLQKMKETAERYLGETVSQAVITVPAYFNDSQRQATKDAGKIA
                     GLEVLRIINEPTAASLAYGLEKKGTGIIAVYDLGGGTFDISILEIGDGVFEVKATNGD
                     TFLGGEDFDIRLVDYLAGEFKKEQGIDLRTDKLALQRLKEAAEKAKIELSSTTQTEIN
                     LPFITADQTGPKHLTMKLTRAKLEALVEDLVQRTVAPCKAALKDAGIAASEIDDVVLV
                     GGMTRMPKVIETVKTFFGREPHKGVNPDEVVAVGAAIQGAVLRGEVKDVLLLDVTPLS
                     LGIETLGGVFTRLIDRNTTIPTKKSQIFSTAEDNQTAVTIRVFQGEREIAADNKALGQ
                     FDLVGIPPSPRGVPQIEVTFDIDANGIVNVQAKDKATGKEQQIRIQASGGLTDSEIER
                     MVKEAEAHAGEDRKRRDLIDARNHADAVIHSTEKALKEHGDRLASADKSAIEESVQAL
                     RTAAEGDDTDAIRAKTDALQQAAMKLGEAMYKAAQAEEAGTGTGDGGEAAGAGSEGGD
                     ETVVDADFEEVRDEQRGES"
     gene            14702..15823
                     /gene="dnaJ"
                     /locus_tag="VGB88_15070"
     CDS             14702..15823
                     /gene="dnaJ"
                     /locus_tag="VGB88_15070"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9648826.1"
                     /translation="MAKRDYYDTLGVARGATAAEIKSAYRKLAMRFHPDRNPDEPDAE
                     HRFKEAAEAYEVLRDEDKRAAYDRFGHDAFDGAGAAGAGFGFTASSFADVFDDLFGEF
                     MGARRGGARRGADLRYNLEISLEDAYRGRQATIRTTTTVTCEACAGSGAEAGSQPIAC
                     TTCQGAGKVRAQQGFFTIERTCPRCQGAGRIIEHPCAACHGTGRQAREKQLQVNVPRG
                     VEDGTRIRLAGEGEAGVRGGPPGDLYIFVSIAPHRLFQRDGGNLFCRVPVAMTSAALG
                     GAIEVPTIEGKRARVTVPAGTQPGHQFRLKGKGMPALRGAGLGDMFVQVTVETPVSLT
                     ERQKELLKEFDAAGAPETHSPEAHGFFTKVKELWDDLRE"
BASE COUNT         2146 a         5802 c         5863 g         2012 t
ORIGIN      
        1 gagcgcctcg gcgacgtggc ggcgggtgac gccgttcgcg ccgtcgaggt cggcgagggt
       61 gcgcgcgacg cgcagcaccc ggtggtagcc gcgcgccgac aatctgaggc ggtcgaccgc
      121 gtccgtgagc agcgcccggc cgcgctcgtc cggcgccgcg acgcgctcca ggagcgcgcc
      181 gtcggcgtcc gaattggtgc gcaccggcgc cccgcccgcg cgcgtctcgc cggcatagcg
      241 ctcggactgc accgcgcgcg cctcggcgac gcgcttgccg acctgggccg agccttccgc
      301 cggcggcggc agcgcgaggt cggcggcggc gagcgcgggc acgtcggcgt gcaggtcgat
      361 gcggtcgaac atcggccccg agatgcgcgc ctggtagtcg gcggcgcagc ggggcgcgcg
      421 gctgcaggcg cgctcggcgt cgtcgagatg gccgcagcgg cacgggttca tcgccgcgac
      481 cagctggaag cgcgcgggat aggtgtagtg ggcgttggcg cgcgcgaccg tcaccgtgcc
      541 ggtctccaac ggctggcgca gcgcctccag caccgggcgc tggtactcgg ggaactcgtc
      601 gaggaacagc acgccgagat gggcgagcga gacctcgccc gggcgcgcgc gcgtcccgcc
      661 gccgaccatc gccgccaccg aggccgagaa gtgcgggtcg cggaacgggc gccggcgcag
      721 caggcgcccg ccctgaagcg cgccggcgat cgaggcgatc atgctcacct cgagcgcctc
      781 cgaggaggcg agcggcggca acaggtcgcc gaggcgcgcg gcgagcatcg acttgccggc
      841 gccgggcggg ccgatcatca gcaggttgtg gccgccggcg gcggcgacct cgagcgcgcg
      901 cttggcggtc tcctggccct tgatgtcggc gaggtcgagc ggcccggccg ccgccgcctg
      961 catcagcggc gcgggcgggg tcagcacctg gctgcccttg aagtggttga ccagggcgac
     1021 gaggtcgggc gcggcgagca cctcgatctc gcccgcccac gccgcctcgc cgccgagcgc
     1081 ggccgggcag atcagcccgc gccccgcggc cgaggccgcg atcgccgccg gcagcacgcc
     1141 gggcaccggc gagatgcggc cgtcgagcgc gagctcgccg agcgccgcga agccgtcgag
     1201 cgcgtccgcc ggcaggatcg ccatcgccgc caggatcgcg agcgcgatcg gcaggtcgaa
     1261 gtggctgccc tccttggcga ggtcggcggg cgcgaggttg accgtgatcc gcttggccgg
     1321 caaggacagc ccgagcgcgc cgagcgccgc gcgcacccgc tcgcgcgatt ccgcgaccgc
     1381 cttgtcgggc aggccgacca gggtgaacgc cgacagcccg gcggcgatct gcacctggac
     1441 ctcgacgccg accacgtcga tgccctggaa cgcgaccgta tggcagcgcg cgaccatggc
     1501 ggcagtctag ggcgcggccg ggatgcgggc cagcgcggcg gcaaccacgc gcggcccgcc
     1561 cgctgttagg ctcacgcgga gccgcgaagg acgcggcggg cgccatgctg cccggccggc
     1621 gcttggtatg tttgaaccac agaggacaca gagcacacag agaaaacata cctctgtggc
     1681 ctctgtgtcc tcggtggttt tcctttctgc cttctccgcg tcccccgctc gttggccagg
     1741 gcaggcctcg acggccccgc gtgcgtacag cctcttcgcc gccttgccgc gccgtgctat
     1801 gcggacttgc ggcgctgcgc ggcgccgcgg cgagggagcg tatcgatggt caagggcctg
     1861 ctggcgctcg cggcgatcat cgccggcacc atggtgctcc agctcgccaa cgctatcctc
     1921 ggcatcatcg tgccgctgca gatgggcctc ttgggcaagt cgaccgcggt gatcggcgcg
     1981 gtggtcacgg cctacgcgct cggcttcctg ctcggctgcc tgggcgcgcc cgccttcatc
     2041 cgcccgattg gccacatccg cgccttcgcc gcgctcgccg ccaccttgtc ggtggcgacc
     2101 ttgttgttca ccaccaccga ctccccgctc gcctgggcgg tgctgcgctt cgccatgggc
     2161 gcgtgcgtcg cggcggtgtt caccgtggtc gagagctgga tcaccggcga ggcgcccaag
     2221 ggcgaccgcg gccgggtgat cgcggtctac atggtgtgca acaagaccgg ggtcatgctc
     2281 ggccagggct tgttggtcgt gggcgacgcg gcgagcgcgg ggttcttcgt gttcgtgtgc
     2341 gcgtgcttct cgttctccct ggtgccggtg gcgctgaccc gcgccggcgg accggtgccc
     2401 caggacgcgg tcaccctcgg catccgcgcg ctgtaccgga tcgcgccggt cggcgtggtc
     2461 ggctgcctcg gcgccgggct ggtgaacgcc tcggtgctcg ggctcgcgcc ggtctacggc
     2521 ctcggcatcg ggcttgcgcc cgagcgcatc gcgctgctgg tgctggtcgc ccagctcggc
     2581 agcctgctgc tgcaatggcc gctcggctgg ctgtcggacc ggatcgaccg ccgccaggtg
     2641 atcgtcggcg cgtgcgcgac cggcgccgcg gcgagcctcg ccatcgcgct ctgggggcgc
     2701 gacgacccgc tgctgctgct cgggctgttc gcgctgtggg gcgcattctc gctgtcgatc
     2761 tacgcggtgt gcatcgcgca tgcgagcgac ttcgccaagc ccgccgagct gttgccgctg
     2821 atcagctcgc tcctggtcgc gtgggcgatc ggctcgacgg tcgggccgcc cttggcgacg
     2881 ctcgccatga ccgcggcggg cgaggacggg ctgttcgcct acgccgccct ggtcaccgcg
     2941 ctgatcggcg ccttcgccgg ctggcggatg acccgccgcc gcgcgccgcc gcgcgcgttg
     3001 cgcgaggcgt tcgtcaacat gcccgcgacc tcgcccgcgg tcgccgagct gtcgcccagg
     3061 gtcgggcgcg agacggacgc ggagtagccg ccgcgctcag gtgccggcgg cgacgcgcgc
     3121 ctcgatcgcg tcccagatga gggcggcgac gttggtgccg tcgaagcgcg cgatctcctg
     3181 gatgccggtc ggcgaggtga cgttgatctc ggtcagccag ccgccgatca cgtcgatgcc
     3241 ggtgaagatc agcccgcgcg ccttcagctc gggccccaag gcggcgcaga tctcgcgctc
     3301 gcgcgcggtc agggtcgcgg gctccgcccg ggcgccgaca tggaggttcg cgcgcgcctc
     3361 gcccgcggcc ggcacccggt tgagcgcgcc gaccggctcg ccgtcgacca ggatgatgcg
     3421 cttgtcgccc cggcgcacct cgggcaggta cttctggacc acgatcggct cgcgatagag
     3481 gccggtgaac agctccaaga gcgcgttcag gttctcgtcc ccggggccga cgtggaacac
     3541 gccggcgccg ccgttgccga acaagggctt gatgatgatg tcgcggtggc gggcgcggaa
     3601 gtcggcgacc tggcgcctgt ccgaggtgat cagggtcggc ggcatcagcc cctcgaaatg
     3661 ggtgacgaac agcttctcgg gcgcgttgcg cacctcggcc gggtcgttga ccacgagcgt
     3721 ctgtgggtgg atgtgctcca acagatgcgt cgcggtgatg taggccatgt cgaacggcgg
     3781 gtcctggcgc atcagcacca cgtccatggt ggagagcgcg acggtctcgg gcgggcccaa
     3841 ggtgaagtgg tcgcccttct tgcgccgcac ttgaagcgcg cgcgcctcgg cgaccacctt
     3901 gctctcgacc atcgacagcg cgcgcggcag gaaatggacc agcgcgtggc cgcgcgcctg
     3961 cgcctcgagc gcgagcgcga acgtcgagtc ggcatcgatg tcgatgcccg cgagcgggtc
     4021 catctggatc gcgaccttga ggcccatcgc gcctccggcc cgccctctgc gcgctcagtt
     4081 gagccggccg cgcaggctcg cgagcagcgc gctcgcctcc tcgcgctcgg cgcccggcgg
     4141 gctgcgctcg aggaaccgcg ccagcgccca ggcggcgtcg cgcaagctgc cgcgccgggc
     4201 cgacaggatg gcgagctcgc gcaacagccc gccggagtcg ggtgccagga tcagcatcgc
     4261 gcgcagcgcg ccgatcgcgc ggcccacgtc gccgcgctgg aaggcgcgca gcttgacgtt
     4321 gttctcgagc ctgagcagca cctcggcgtc cgagaccgcg gcatgatggt gaggcaggag
     4381 ctcggcctcg ggaccggcgg tggccttgag caggtcgcgc aaatcgccgc tgccgaccgt
     4441 ccggcagcgg tggaacggat cgaggatcgc gcgcccgctc gaatcctcga tgcggatcag
     4501 gaaatggccg ggaaaggcga gcccgacggc gccccgcccg ccggcgcgca gccagtgcag
     4561 gcacaggatg ccgaggctca ccgggatgcc gcgccggcgg tcgatgaccc gcatcaggtt
     4621 ggcgttgtcg ggatgctcgt agtcgtcgcg gtcgccgtca tagccgtagc cgtcgaacag
     4681 gctggcggcg agcgcgcgcg ccgcgccttc gaccgtctcg gccgccgcgc cggcgcgcgc
     4741 ctcggcggcg agcgcgcgaa gatgggcgcg atagggcgcg gggtcggtct cggggcggtc
     4801 gatcgcggcc agcagcagcg cctgctcggc gatccccggc ccggcctcgc cggcccgcac
     4861 cgcgccgcgc agcgcctcct cctcggccga cgccggcgcc ggcagctcgg cgatcatccg
     4921 cgccgcgccg cgtcgtcctt gaagacgaca tggctgacca cgcgccgcac atgctcgagg
     4981 gtgcgcgcat gcgcgatcac ccggtcgagc tccgcctggt cctgggcgat gccgagcaga
     5041 tagacggtgc cgttgacggt ggtgatgctg taattgatgt cgagcacctg gcggtcggtg
     5101 agcaggtggc cgcgcagctt ggtggtgatc cagcggtcgc gcaccacatc gaccgcggtg
     5161 tcgtcgccga tgcggatctc gttgatcacc tggcgcacgc cgtccacttt ccacgccagc
     5221 tcggcggcgc ggtcgaccag ttcctggccg cgcaccgtgc cgctcagcag cacccggccc
     5281 tcgaccacgt cgatgttgac ctcgcggtac aggtcgtcga tatgcgccat gaacagcgcg
     5341 tggttgatct cgaggtcgat cgcggtatcg gataccgcct cgccgaccgg gcgttcctgg
     5401 gcgacggcga cgccggcggt ggcgccggcc ccggtcgcga cgccgagcgg cgagcatgag
     5461 ctgagcaccg gccccgcggc cagcgccgcc agcagggcaa gcgccgcggc gcgccggcgc
     5521 ctcaccaagc cgactcgcgc cacgcgtcgg gatgatgcac gggccatagg aaacgcgggc
     5581 tgggccaagg tctgacgagc atgacgtcga agcgtgcaca gagtgcgccg aagcgggggt
     5641 tgcgtgcaag gtacaattcc gccgcgcgca cgatgcgccg gcgctggcgc ggcgcgggcg
     5701 gcgcagtctc ggcgtcgatg tcggcgcgcg ccttgacctc gacgaacgcg atcaccggcc
     5761 cgcgccgggc gatgatgtcg atctcgccga ccggcgtgcg ccagtcgcgg gcgaggatct
     5821 gccagccgca aagcctgagg cgccagcggc acgccgcctc ggcggtgcgg ccgaagcgca
     5881 gcgcgccgtg gcgcgcggcg cgccggtcag tccgcggcgt cgccaccgcg cagcgccagc
     5941 gcgcgcgcat acagcgccct gcgcgcccgc ccggtgcgct gggccacggc ctgcgcggcg
     6001 cgcgccggcg gcagctcggc gagcgccgcg ccgagcagcc ggtcgacctc ggcatcgctc
     6061 accgcctgcc ccgcgggctc ggccggcgcc ccgatcacca gcacgcactc gccgcgcggg
     6121 gcccggtccg cgaaccgccc ggcgagcgca tcgagggggg cgcgcaccac ctcctcgtgc
     6181 agcttggtca gctcgcgcgc gaccgccgcc ggccgcgcgc cgagcgccgc cgccgcgtcg
     6241 gcaagcaggg cgccgagccg gcgcggcgat tcgagcagca ccaaggtggc gtcgatcgcg
     6301 gcgatgcgtg ccagcgccgc gcgccgcgcc tgggcgcgcg ccggcagata gccggcaaag
     6361 taaaacctgt cggtgggcag gcccgagacg gtgagcgccg ccagcaccgc cgacggcccg
     6421 ggcacgggat ggaccgggac gccggcggcg atcgcctcgg tgaccagccg gtagccgggg
     6481 tccgagacca gcggcgtgcc ggcatcgctg accaggcaaa cgattgcccc ggcgtgcaac
     6541 tgcgcgatca ggcgcgggcg cacgcggggc gcgttgtggt cgttgtagct caccagcttg
     6601 gccgcgacgc cgtgggcggc gagcagccgg ccggtgtggc gggtgtcctc gcacgccacc
     6661 agatcggcgc cggcgagcgc ggcgagcgcg cgcagggtga tgtcggccag gttgccgatc
     6721 ggggtcgcga ccagatacag cccggggtcc agtttacttg gccggtgccc cgcgctaccg
     6781 tcgccgcgtg atcgcgcttt cgccacgccg tcgtcgcgtc aggcgcgcgc ttagccccgg
     6841 ggcattggcc cgccgggccg cgtgcctgct ccttgggtta atattggcgg ccggatgcgc
     6901 gcgcaccgta gcgccgccgc aaccggcagc gcaagcggag gccgaggccg ccgcgcccgc
     6961 gccggcgccg gccgaggccg agccggtggt ctcgccgctg gcgccgccgt ccgcggtgcc
     7021 ggtcgcgccg ccggggcggc cgctggccgc gggcgaggtc cgggtcggca tcctgctgcc
     7081 cttcaccggc gcccatgcgg cgctcggcga ggcgctgctc aacgccgcct cgctcgcctt
     7141 gttcgaccac ggcggcgaca ccgtgacctt gctgcccaag gacaccggca gcaccggctc
     7201 gggcgccgat tcggcggtgc gcgccgcgct cggcgagggc gcgcgggtgg tggtcggtcc
     7261 gctgctggca agctcggtgc gcgccgccgc gcccgcggcc cgccaggcgg cggtgccggt
     7321 gctggcgttc tccaacgaca gctcggtggc gggcgagggg gtgttcatca tgggcgtgac
     7381 cccggcgcag caggtcgcgc gggtggtcgc gctggcccgc gcccagggcc acgcgcgctt
     7441 cgccgcgctg gcccccgaca cgccgttcgg caacgcggtg gtgagcgcgc tgttcgaggc
     7501 cgcggaggcc tccggcggcg cggtcgaccg ggtcgagctc ttcgtccccg gccaggtcag
     7561 cgtgagcgag aacgaggagg tccgccggct cgccgcggcg gccaagggcg gcgcgttcga
     7621 tgcgctcttg atcgcagccg gcggcgccga cctgcgcacg atggcgccgc tgctgcccta
     7681 ttacggcgtc gacccggacc ggattcagct gctcggcacc gagctgtggc acgaccccgc
     7741 cgtgctcggc gagccggcgc tgctcggcgg ctggttcgcc gcccagcccg atgacgcctt
     7801 cgaccagttc cgcggccgct accggtcggt ctacggcgcc gagccgccca ggctcgccgc
     7861 gctcgcctac gacgcggtgg cgctggtcgc ggcgctcgcg cgcagcccac gcggccccga
     7921 cttcagccgc gccgtgctca ccgatgccga cggcttcgcc ggcatcgacg gcatcttccg
     7981 gttcggcgac gacggcgtga accagcgcgg gctcgcggtc taccgcatcg ccgcgcgcac
     8041 cttcgaggtg gtcgacccgg cgccggcctc gttcgccgcg cccgcgttct gatccgccgc
     8101 gcccgctcgc ccgggcgtga ggcgcgcgac gatcgcatcg agcagcaggc ggccctgggg
     8161 cgtcgtcgcg atccggccgg ggcggagccg gatcaggcga gcgtcggcga gcgcggccac
     8221 cgcccgggca tcgagcgcct cggcgaaagc gaggccggtg cggcggcgga ggtccgcctc
     8281 ggccacaccg tcagtgagcc ggagccccat cagcagcacc tccgcgacca gctcggctgc
     8341 cgtgagcgcc gcgcggcggc gccggccgtg gccgcgcgcc tccaccgcgg cgagccaggc
     8401 cgcgggcgcc ggaatggtct cggtcgccac cacccggccc gcgtgcgcga ggcggccgtg
     8461 ggcgccgggg ccgatgccgg catagtcgcg gtgccgccag tacgccaggt tgtggcggca
     8521 ttcctcgccg ggccgcgcgt ggttcgagat ctcgtaagcc ggcaggcccg ccgcctccag
     8581 cacctcctgg gtcacctgcc acagcgccac cgccgtgtcc tcgtcgggcg acgcgaattc
     8641 gccggcgcgg cgcgcggcga agaacggcgt ccccttctcg atggtcagct gataggccga
     8701 gacgtggccc tgcgcgtgcg ccagcgcgcg catcagctcg gcccgccacg cggcgggggt
     8761 ctggccgggg cgggcgtaga tgaggtcgaa cgagacgcgc gccaccgcgg cccgcgccgc
     8821 cgccaccgcc gccagcgcct cgcccgcgtc gtgggcgcgc cccaggaagc gcagcgcggc
     8881 gttgtcgagc gcctggacgc caagcgagat gcggttgatg ccggcggcgg cgaagtcggc
     8941 gagccgcgcc gcctcggccg atgtcgggtt ggcctcgagc gtgatctcgg catcgccggc
     9001 gaggtcgaag cggcgcgcga tgcggtcgat cagcgcggca acggtggccg gagccatcag
     9061 cgacggcgtg ccgccgccga ggaacaccgt ctgcaccgcg cgcgcgccga tccgatcggc
     9121 ggcctcgtcg agctcggcga gcagcgcgcg ccgccagcgc ccctggtcga tgccctcggc
     9181 gacgtggctg ttgaagtcgc aataggggca tttcgaccgg cagaacggcc agtggacata
     9241 gaccgacagc gcgcgctcag cggcggccaa ccgaggactc ctccgctgcc ggcgcgaggc
     9301 aggcggcggc gagcttggcg aaggcgcggg cgcggtggct gatgcggtcc ttgtccgccg
     9361 gcgccatctc ggcgaaggtg cggcgatggc cggcggcaac gaagatcggg tcgtagccga
     9421 agccgcggcc cccgcgcggc gggaaggcga gccggccctc gaccgtgccc tcgaacagct
     9481 cgcagtggcc gtcgggccag cacagcgcga gcgcgcacgc gaaccgggcc tcggcgtccc
     9541 tgcgcgcgcc cagctcggcc tcgattcgcg ccatcgcccg agcgaagtcg cgctccgggc
     9601 ccgcccagcg cgccgagtgg agtcccggcg cgccggcgag cgcggcgacc tcgagcccgg
     9661 aatcgtcggc gagcgcgggc aggcccgcgg cgcgcgcggc ggcctcggcc ttgatgcgcg
     9721 cattggcggc gaagctcgcg ccggtctcgg ccggctcggc gaggccgagc ccggacgccg
     9781 ccgccgcctc gatcccgaac ggtgcgagca gggcggcgat ctcggccacc ttgccggggt
     9841 tgtggctcgc caccagcacg cgggggccgt caagccggcg cgccatcgcg tgccgcgagc
     9901 gcgcgcgcga tcgcctcgcc ctggagcgcg accagctgct cggtgccggc gcgcgcgagc
     9961 gccagcagaa ggtcgagatg ctcggcgcgg aacggcgccg cctcggcggt cgcctggacc
    10021 tcgacgatgc cgcccttggc ggtgagcacg aagttggcgt cggcttccgc ggtgctgtcc
    10081 tcggcatagt cgaggtcgat cacggcgatg ccctgataca ggccgcagct gatcgcggcg
    10141 acccggtcgg tgagcggcga gcgctcgatc agggcgttgc gcaccagata gtcgaacgcc
    10201 aggtgcaagg ccacgtagcc gccggtgatc gccgcggtgc gggtgccgcc gtcggcctgc
    10261 agcacgtcgc agtcgacgcg cacctggcgc tcgccgaagc cgtcgagcgc gcacaccgcg
    10321 cgcaaggacc gcccgatcag ccgctggatc tcgagcgtcc gcccggactg gcgcccgcgc
    10381 gccgcctcgc ggtcggtccg cacgtcggtg gcgcgcggca gcatgccgta ctcggcggtc
    10441 acccagccgc gcccggtgtt gcgcaggaac ggcggcaccc ggtcctcgac cgtcgccgcg
    10501 cacagcacct gggtgtcgcc gaagcggacc aggcacgagc cctcggcgta cttgttgacg
    10561 ccggtctcga tcaccacctc gcggagctgg tccggggccc ggccggacgg gcgcatcgcc
    10621 tttgcctcgc tcgaaatcgc gcgcgcgcgc ttgggccgcg accctaatcc ctgccggggc
    10681 gcgcgtccac cgcccgcact gcggtatcat tgacgctggt cggggcggta actaagttct
    10741 ggcgcgccac gcccccgcaa cccgagccca gcaggaggtc tggtgagcac cacccccaag
    10801 tcggccatgt tctcggagct gaacgagcgc tcgcgcctga tcttctcgca gatcgtcgag
    10861 tcctatttcg acaccggcga gccggtcggc tcgcgcacgc tgtcgcgcaa gctgccgatg
    10921 cagctgtcgc cggcgaccat ccgcaacgtc atggccgacc tcgaggacct gggcctcctg
    10981 ttcgcgccgc acacctcggc cggccggctg ccgaccgagc gcggcctgcg cctgttcatc
    11041 gacgggctgc tggagatcgg cgacctcagc gccgaggagc gcgacaacat cgaaagccag
    11101 tgcgcgggcg ccgggcggac cctgtccgag gtgctcaacc aggcgaccga gatgctgtcg
    11161 ggcctgacca gcgccgccgg cctggtgctg gcgcccaaga gcgaggcgcc ggtgcgccat
    11221 ctcgagttcg tcggcatgtc gccgggccgg gcgctggcga tcatggtgct ggccaacggc
    11281 ctggtcgaga accgcctgat cgaccttccg gtgggcatca ccgcctcggc gctggtcgag
    11341 gccgcgaact acatcaactc ccgcctgatc gggcgcacgc tcggcgaggc acgcggcatg
    11401 atcgaggtcg agctcgcggc gcgcagggcc gagctcgacg cgctcgcgac ccgggtggtc
    11461 gaggccgggc tcgccaccct cgccaatgtc tcggacgagc cgaccctgat cgtgcgcggg
    11521 cgcgccaacc tgttcgagga cgtgagcgcg ctggccgacc tcgagcgcat ccgcagcctg
    11581 ttcgaccagc tcgagcagcg caagaacctg ctcaggctga tcgactcgac cctcggcggg
    11641 ccgggggtgc acgtgttcat cggctcggaa agcaagctgt tcgacctcgc cggctgctcg
    11701 atgatcgtcg cccccttggg cgccaagcgc ggcctcggcg acggctcggc cgccggcgcc
    11761 tgcgtcggcg cgatcggcgt catcgggccg acccggatca actacgcgcg catcatcccg
    11821 atggtcgact acactgcgcg ggcgatcggc cggctgatgt cctgaccgcg ggcttgcgcc
    11881 gccgcgttca atcgcaacac aggcagacgg agtcatggcc gaacccaagc cgcaatcgga
    11941 cgcgcccgag ccgcaagcgc ccgccgaggc cgaaggcgtg cccgccggcg agacgccgcc
    12001 ggcggcggag gagctgctgc gcgcggagct ggccgaactc aaggaccagc tgttgcgcgc
    12061 gctcgcggaa gcggacaatc agcgcaagcg cgcggcgcgc gagcgcgacg agaccgccaa
    12121 gttcgcgatc gccggctttg cccgcgacgt ggtccaggtc gccgacgatc tcgagcgcgc
    12181 gctggccagc gcgccgcgcg ccggcggcga cggccaggca tcgatcgcgt cgctggtcga
    12241 gggcgtggag ctgacccggc gcagcctgca ggcggcgctc gaccgccacg gggtgaggcg
    12301 catcgagccc ttgggcgagc ggttcgacca caacctgcac gaggccatgt tcgaggtcga
    12361 gaccgccgac gccgagcccg gcaccgtggt ccaggtggtc cagcacggct accgcatcca
    12421 cgaccggctg ctgcgcgcgg cgcgggtcgc cgtcgccaag ccgccggcgc ccggagcggg
    12481 cgtcgatacc gaggcctgag cccgcgccgg caccggttgc aggcccagct cccggctccc
    12541 atatacccca acgatcggag gtttgccggt acccacgtga aaccgaccgg gccgcccggt
    12601 gctcgctaag aggccggggc cggtccgcct gctttgctga caaggggaaa gtcatgagca
    12661 agatcatcgg tatcgacctt ggcaccacca actcctgcgt cgcggtgatg gagggcaagg
    12721 acgccaaggt gatcgagaac gcggagggca tgcgcaccac gccctcgatc gtcgccttca
    12781 ccgacgacga ggtcctggtc ggccagccgg ccaagcgcca ggcggtgacc aaccccgaga
    12841 acacgctgag cgcgatcaag cgcctgatcg ggcggcgctg ggacgacccg atggtgcgca
    12901 aggacgccgg catggtgccc tacaagatcg tcaaggccga caacggcgac gcctgggtcg
    12961 aggccggcgg caagcgccac gcgccgagcc agatcagcgc gctggtgctg cagaagatga
    13021 aggagaccgc cgagcgctat ctgggcgaga ccgtcagcca ggcggtgatc acggtcccgg
    13081 cctacttcaa cgattcccag cgccaggcga ccaaggacgc cggcaagatc gccgggctcg
    13141 aggtgctgcg catcatcaac gagccgaccg cggcctcgct cgcctacggc ctggagaaga
    13201 agggcacggg catcatcgcg gtctacgacc tcggcggcgg caccttcgac atctcgatcc
    13261 tcgagatcgg cgacggcgtg ttcgaggtca aggcgaccaa cggcgacacc ttcctcggcg
    13321 gcgaggactt cgacatccgc ctggtcgact acctggccgg cgagttcaag aaggagcagg
    13381 gcatcgacct gcgcaccgac aagctggcgc tgcagcggct caaggaggcg gccgagaagg
    13441 ccaagatcga gctgtccagc accacccaga ccgagatcaa cctgccgttc atcaccgccg
    13501 accagaccgg gcccaagcac ctgaccatga agctcacccg cgccaagctc gaggcgctgg
    13561 tcgaggacct ggtccagcgc acggtcgcgc cgtgcaaggc cgcgctcaag gacgccggca
    13621 tcgccgcgag cgagatcgac gacgtggtgt tggtcggcgg catgacccgt atgcccaagg
    13681 tgatcgagac ggtcaagacc ttcttcgggc gcgagccgca caagggcgtc aacccggacg
    13741 aggtggtcgc ggtcggcgcc gccatccagg gcgcggtgct gcgcggcgag gtcaaggacg
    13801 tgctgctgct cgacgtgacg ccgctgtcgc tcggcatcga gaccttgggc ggcgtgttca
    13861 cccgcctgat cgaccgcaac accaccatcc cgaccaagaa gagccaaatc ttctcgaccg
    13921 ccgaggacaa ccagaccgcg gtcaccatcc gcgtgttcca gggcgagcgc gagatcgccg
    13981 ccgacaacaa ggcgctcggc cagttcgacc tggtcggcat cccgccgtcg ccgcgcggcg
    14041 tgccgcagat cgaggtcacc ttcgacatcg acgccaacgg catcgtcaac gtccaggcca
    14101 aggacaaggc gaccggcaag gagcagcaga tccgcatcca ggcctcgggc gggctgaccg
    14161 attccgagat cgagcgcatg gtcaaggagg ccgaggcgca cgccggcgag gaccgcaagc
    14221 ggcgcgatct gatcgacgcg cgcaaccatg cggacgcggt catccactcg accgagaagg
    14281 cgctcaagga gcacggcgac cggcttgcca gcgccgacaa gagcgcgatc gaggagtcgg
    14341 tccaggcctt gcgcacggcc gcggaaggcg acgacacgga cgcgatcagg gccaagaccg
    14401 acgccctcca gcaggcggcg atgaagctcg gcgaggcgat gtacaaggcc gcccaggccg
    14461 aggaggccgg caccggcacc ggcgacggcg gcgaggccgc gggcgccggc agcgagggcg
    14521 gcgacgagac cgtggtcgac gccgacttcg aggaggtccg cgacgagcaa cggggcgagt
    14581 cttgaccgcg gcgcccatga cgactgcggc gcccgtggcc gcgggccggc catgaccagg
    14641 gttcgccgtc ccggcgggac ggcctgagcc ggtcgaggcg tccgtcgggg gtcgggcggc
    14701 gatggccaag cgcgattact acgacacgct cggcgtggcg cgcggcgcca ccgcggccga
    14761 gatcaagtcg gcgtatcgca agctggcgat gcgtttccat cccgaccgca acccggacga
    14821 acccgacgcc gagcaccgct tcaaggaggc cgccgaggct tacgaggtgc tgcgcgacga
    14881 ggacaagcgc gccgcctatg accgcttcgg ccatgacgcc ttcgacggcg ccggcgcggc
    14941 cggcgccggc ttcggcttca ccgcgtcgag cttcgccgac gtgttcgacg acctgttcgg
    15001 cgagttcatg ggcgcgcgcc gcggtggcgc ccggcgcggc gccgatctcc gctacaacct
    15061 cgagatcagc ctcgaggacg cctatcgcgg ccgccaggcc accatccgca ccaccaccac
    15121 cgtgacctgc gaggcctgcg ccggctcggg cgccgaggcc ggctcgcagc cgatcgcctg
    15181 caccacctgc cagggcgcgg gcaaggtgcg cgcccagcag ggcttcttca ccatcgagcg
    15241 cacctgcccg cgctgccagg gggcgggacg gatcatcgag cacccgtgcg ccgcctgcca
    15301 cggcaccggc cgccaggcgc gcgagaagca gctccaggtc aacgtgccgc gcggggtcga
    15361 ggacggcacc cgcatccggc ttgccggcga gggcgaggcc ggggtgcgcg gcgggccgcc
    15421 gggcgacctc tatatcttcg tcagcatcgc gccccatcgc ctgttccagc gcgacggcgg
    15481 caacctgttc tgccgggtgc cggtggcgat gaccagcgcc gcgctcggcg gcgccatcga
    15541 ggtgccgacc atcgagggca agcgcgcgcg cgtcaccgtg cccgcgggca cccagcccgg
    15601 ccaccagttc cggctcaagg gcaagggcat gccggcgctg cgcggcgcag gcctcggcga
    15661 catgttcgtc caggtcacgg tcgagacccc ggtcagcctg accgagcgcc agaaggagct
    15721 gctgaaggag ttcgacgccg ccggcgcgcc cgagacccac agccccgagg cgcacggctt
    15781 cttcaccaag gtcaaggagc tgtgggacga cctgagggag tag
//
LOCUS       DASWHU010000409         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2540600, whole genome shotgun sequence.
ACCESSION   DASWHU010000409 DASWHU010000000
VERSION     DASWHU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540600"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2829
                     /locus_tag="VGB88_15075"
     CDS             <1..2829
                     /locus_tag="VGB88_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648827.1"
                     /translation="MRTRVAALDERWQGRIAGGLSLHIGVNTGPVVAGDLGSGDASAY
                     AVTGDTVNTAARLLSAAGDNEILVGPTTHQLMQHRFAFEPVGALAVKGKSEPVTAFRL
                     VGEAAVVNMPRGLASYGLAAPMVGRERDIDQLAACFEQALTGRAQVVRIVGDAGAGKT
                     RLVEEFLLRLAQSRRLDGVAVRRSACSALADQTYGVLASLVREGYGIAPGDDIEVARA
                     KLISGFHSIGVGDDDARQMARFMGHVLGLYARDDADLANLEPEQFKRQVFMAARTMFE
                     RRLASGPVVLVVDDLHWADSASIELLRFLVDRLDDRKLLLLLTHRPGFDVSTLATSRT
                     SQVARRLAPLSAKDCESVLAGMFGESVSRIPAGVRRFVIERGSGNPFYLEEIVRSLID
                     GGMLVRKDDGWHCVQSVTAQDLPLTIQGMLLSRLDRLADEVRQVTHEAAVLGTEFDAA
                     LLSAMSGLPDAVEGSLEALQDAELIEQVTRGRPGAEAGLAADRHYRFSHAIVQEVVYQ
                     NLLVRRRGELHGEAGRALEKLCAGRVERLEDVEALARHFSRSAEKVKGVRYLVRAGDW
                     ARRIFANDDAAKHYQLALQTIDECRGCEQERPGLVERLGDVLSTLGRRDQALGHYETV
                     LAAAAAAGDAVGEARLQRKIAAQHWEAGARGVALERIKAALALLQDQGQDHVELAHLY
                     QEMGRLAFRSGDNAGAIDWAGRALQQAERLLARASGNGADRGAAMEAAAVVAEAYNTL
                     GVARARMGDPQQAVADVERSLEVAERHGLHTVACRGYTNLSVLYSTLDPGKAIETCQR
                     GLAVAKKIGDLGFQSRLYANLGVSYCTFTGRCEGEGVDAVRRAIDLDRQLGLVDHLPV
                     SLVVLGQIYQCHGQPLVAVDLYKEALDLAEDLDEPQILFPCYDGLATVHLDLDDIEAA
                     EGYMEKAQAVCERAGLDPESLVVLPFLC"
BASE COUNT          406 a          979 c         1053 g          408 t
ORIGIN      
        1 atgcgcacgc gggtggcggc gctggacgag cgctggcaag ggcgcatcgc cggcgggctg
       61 agcctgcaca tcggcgtcaa taccgggccg gtggtggcgg gggacctcgg ctcgggcgac
      121 gcctcggcct atgcggtcac cggcgacacg gtcaacaccg cggcgcgctt gttgtcggcg
      181 gccggcgaca acgagatcct ggtcgggccg accacccacc agctcatgca gcaccgtttc
      241 gccttcgagc cggtgggcgc gctcgcggtg aagggcaagt cggagccggt gacggcgttc
      301 cgcctggtcg gcgaggccgc cgtggtcaac atgccgcgcg ggctcgccag ctacgggctg
      361 gcggcgccga tggtcgggcg cgagcgcgac atcgaccagc tcgcggcgtg tttcgagcag
      421 gcgctcaccg gccgcgccca ggtggtgcgg atcgtcggcg acgcgggcgc cggcaagacc
      481 cgcctggtcg aggagttcct gctccggctc gcccaatcgc ggcggctcga tggcgtcgcg
      541 gtgcgccgca gcgcctgctc ggcgctcgcc gaccagacct acggcgtgct cgccagcctg
      601 gtgcgcgaag gctacggcat cgcccccggc gacgacatcg aggtcgcccg cgccaagctg
      661 atctccggct tccactcgat cggcgtcggt gacgacgacg cgcggcaaat ggcgcgcttc
      721 atgggtcacg tgctgggcct gtacgcgcgc gacgacgccg atcttgccaa cctcgagccg
      781 gagcagttca agcgccaggt gttcatggcc gcgcggacga tgttcgagcg ccggctggcg
      841 tcgggcccgg tggtgctggt ggtcgacgac ctgcattggg cggattccgc ctcgatcgag
      901 ctgctccgct tcctggtcga tcgcctggac gatcgcaagc tgctgctgct gctcactcac
      961 cggcccggct tcgacgtcag cacgctggcc accagccgca cctcgcaggt cgcgcggcgg
     1021 cttgcaccgc tgagcgcgaa ggactgcgag agcgtgctcg ccggcatgtt cggcgagtcg
     1081 gtgtcgcgaa tcccggccgg cgtgcgccgg ttcgtgatcg agcgcggcag cggcaatccg
     1141 ttctatcttg aagagatcgt gcgcagcctg atcgacggcg gcatgctggt gcgcaaggac
     1201 gacggctggc attgcgtgca gtcagtcacc gcccaagact tgccgctgac catccagggg
     1261 atgctgctgt cgcgcctcga ccggctggcc gacgaggtgc ggcaggtcac gcacgaagcg
     1321 gccgtgctcg gcaccgagtt cgacgccgcg ctgctctcgg cgatgagcgg cctgccggac
     1381 gcggtcgagg gcagcctgga agcgttgcag gacgccgagc tgatcgagca ggtgacgcgc
     1441 ggccggccgg gcgccgaggc cggcctcgcc gccgacaggc actatcgttt cagccacgcg
     1501 atcgtccagg aagtggtcta ccagaacctg ctggtccgcc gccggggaga gctgcacggc
     1561 gaggcgggac gggccttgga gaagctctgc gccgggcgcg tcgaacgcct cgaggacgtc
     1621 gaggcgctgg cgcgccattt cagccgcagc gccgagaagg tcaagggcgt gcgctacctg
     1681 gtccgcgccg gcgactgggc gcggcgcatc ttcgccaacg acgatgcggc caagcattat
     1741 cagctcgcgt tgcagaccat cgacgagtgc cgcggctgcg agcaggagcg gccgggcctg
     1801 gtcgagcggc tgggcgatgt gctcagcacg ctggggcggc gcgaccaggc gctcggccat
     1861 tacgagacgg tgcttgccgc ggcggccgcg gccggcgacg cagtcggcga ggcgcgcctg
     1921 cagcgcaaga tcgccgccca gcactgggag gcgggcgcgc gcggcgtggc gctggagcgg
     1981 atcaaggcgg cgcttgccct gcttcaggac cagggccaag accatgtcga gctcgcccat
     2041 ctgtaccagg agatgggccg gcttgcgttc cgcagcggcg acaacgcggg cgcgatcgac
     2101 tgggcgggcc gggcgttgca gcaggccgag agactgctcg cgcgcgcgag cggcaacggc
     2161 gccgaccgcg gcgcggcgat ggaggccgcg gcggtcgtcg ccgaggccta taacacgctc
     2221 ggcgtcgccc gcgcgcgcat gggcgacccg cagcaggcgg tggccgacgt cgagcgcagc
     2281 ctcgaggtcg ccgagcggca cggcctgcac accgtcgcct gccgcggcta caccaatctc
     2341 agcgtactct acagcacgct cgacccgggc aaggcgatcg agacctgcca gcgcggcctg
     2401 gcggtggcca agaagatcgg cgacctcggc ttccagtcgc gcctgtacgc caatctcggc
     2461 gtgtcctact gcaccttcac cggccgctgc gagggcgagg gggtggacgc ggtccgccgc
     2521 gccatcgacc tcgaccgcca gctcgggctg gtcgaccacc tgccggtttc cctggtcgtg
     2581 ctcggccaga tctaccagtg ccacggccag ccgctggtcg cggtcgacct ctacaaggag
     2641 gcgctggacc tggccgagga cctggacgag ccgcagatcc tgttcccgtg ctacgacggc
     2701 ctggcgaccg tccatctgga tctcgacgac atcgaggccg ccgagggata catggagaag
     2761 gcgcaagctg tgtgcgagcg cgccggcctc gatcctgaat cgctggtcgt gctaccgttc
     2821 ctgtgttaac cgtcgaaatg ccggtc
//
LOCUS       DASWHU010000410         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1829368, whole genome shotgun sequence.
ACCESSION   DASWHU010000410 DASWHU010000000
VERSION     DASWHU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829368"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGB88_15080"
     CDS             complement(<1..269)
                     /locus_tag="VGB88_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648828.1"
                     /translation="MTARLVAFALAAGLLGRAAFAAGDYPVDQFEKITQCMSDPSCQV
                     QKEDRIAGSAAEDQSLTYRRPICIGGCMMVCTSDACWCEIDDKCNR"
     gene            complement(366..857)
                     /locus_tag="VGB88_15085"
     CDS             complement(366..857)
                     /locus_tag="VGB88_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX9648829.1"
                     /translation="MIRFVGVCILLAATLTFAPGGTARPAQAGQLGPVDRAQLECLAL
                     NIYFEARSEPLAGQLAIAFTTLNRVADRQFPDTICGVVRQGGDEVKGRCQFSWWCDGK
                     SDRVQEGDAWAEALHVARRALREPAPDPTRGALFFHVAGANPGWARDRELTARIGRHR
                     FYR"
     gene            1201..1833
                     /locus_tag="VGB88_15090"
     CDS             1201..1833
                     /locus_tag="VGB88_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9648830.1"
                     /translation="MIDLYTWSTPNGRKVSILLEDLELPYEVHAVDIGKDEQYDPEFL
                     EISPNNKIPAIVDRDNGMKLMESAAIMIYLAEKTASPLWPTEMEPRFDVLQWLMMQMG
                     SVGPMLGQTHHFVKFNPGKAPYAEQRYRKENARIYAVLDRQLGRRPYLAGDISIADIA
                     TWPWISRFEWQEMDLGAYPNLKRWYLDIAARPATQRGYDVPARGFEIPMP"
     gene            1915..2238
                     /locus_tag="VGB88_15095"
     CDS             1915..2238
                     /locus_tag="VGB88_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648831.1"
                     /translation="MGDKLTPFETFRQLKERQRAEERARVESARGEEARAGLTEIMAV
                     IEDTGRLVGRLQGGLKRELAHALARGEASGVDGMRNLVRRYERLSWELHGQLRAVLEG
                     ARKGR"
     gene            2249..2794
                     /gene="thpR"
                     /locus_tag="VGB88_15100"
     CDS             2249..2794
                     /gene="thpR"
                     /locus_tag="VGB88_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9648832.1"
                     /translation="MAHRLFVGVDLPALVREALHRLQFGVRSARWVAPENLHLTLRFI
                     GDVDGRQAQDIHDALTALNARSFTIELRDAGCFGPGKRPRALWAGVAPSRELDELKLR
                     VDGLLARAGVPPDSRRYWPHVTLARLRNDAAREIGARIAEISPAVAGAFEVEELILFQ
                     SHKSTSGSYYSREAAYPLRPS"
     gene            2818..3393
                     /locus_tag="VGB88_15105"
     CDS             2818..3393
                     /locus_tag="VGB88_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX9648833.1"
                     /translation="MLLEQVRACRLCAAHLPHGPRPVLRARPSARLLIVGQAPGTRVH
                     ETGIPWNDPSGDRLRQWLGLDRARFYDDRTVAIVPIGLCYPGRDAHGGDRPPRPECAP
                     LWHPRLGAELGAVELVLLVGGYAQRYYLAERARPTVTETVRAWRDHLPRFVPTPHPSW
                     RTQAWVRRNPWFESELLAYLRRRVADLLGPA"
     gene            complement(3411..>3791)
                     /locus_tag="VGB88_15110"
     CDS             complement(3411..>3791)
                     /locus_tag="VGB88_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648834.1"
                     /translation="DDRIGGMVTKVGTPLDLPQQEITGRLSPHYDHDGRLTGGRLLVN
                     WPYANAGQPVELAMMRETFIRSGPRINGVGKILHRTTKHTYLAGSLSFDPASHASLSS
                     FYRVSFIAATCYPAHDLPELTKIN"
BASE COUNT          577 a         1299 c         1357 g          558 t
ORIGIN      
        1 cgattgcact tgtcgtcgat ctcgcaccag caggcgtcgg acgtgcacac catcatgcag
       61 ccgccgatgc agatcggccg ccggtaggtg aggctctggt cctcggccgc ggagccggca
      121 atccggtcct ccttctgcac ctggcacgac gggtcgctca tgcactgggt gatcttctcg
      181 aactggtcga ccggatagtc tccggcggcg aatgccgctc gccccagcag gccggcggcg
      241 agcgcaaacg cgaccaagcg cgcggtcatg tcatcctccc ctcgaagcgg gcagtggccc
      301 gatcgcgcga gtatgcggcg ccggcgcggg cgcggcaaca tcgggcgcag ccccggcaag
      361 cgccatcagc gatagaaccg gtgcctgccg atgcgcgcgg tgagctcgcg atcgcgcgcc
      421 cagccgggat tggcgccggc gacgtggaag aacaaggcgc cgcgggtcgg gtcgggggcc
      481 ggttcgcgca gcgcccgccg cgccacgtgg agcgcctcgg cccaagcgtc cccttcctgg
      541 acccggtcgc tcttgccgtc gcaccaccag gagaactggc agcgtccctt gacctcgtcg
      601 ccgccctggc ggacgacgcc gcagatggtg tcgggaaatt gccggtcggc gacgcgattg
      661 agcgtcgtga aggcgatcgc gagctggccg gcgagcggct ccgagcgcgc ctcgaaatag
      721 atgttgagcg ccaggcattc gagctgggcg cggtcgaccg gcccaagctg gcccgcctgt
      781 gccggccgcg ccgtcccgcc cggcgcgaac gtcagcgttg ccgccagcag gatgcagacg
      841 ccgacgaaac ggatcatggc aatgcccccg gtcttgccta tccctcgtcc gccacgatcg
      901 ttctcacctc cgcccgacag tggccatgaa agcgacataa cccggaccgt ggcggcgatc
      961 aagaaaactt gagccgaagt gtgacggcac caattcgcgc atctcggcgc cggccacgcg
     1021 cggttcgcac cgatcggttg gtggtgatac gaattaggaa tggttccttg agtccgagtc
     1081 tcaataacgc cgcggccggc gcctgcgtat ccgttcgcgg gcggaccaac gcgggctggt
     1141 gccgcggcga agctgcggct aacgtctgcg cgcgactgcg caccagccaa cggagccgag
     1201 atgatcgacc tctacacttg gagtacgccg aacgggcgca aggtctcgat actgctcgag
     1261 gacctggagc tgccgtacga ggtccatgcc gtcgatatcg gcaaggacga gcagtacgat
     1321 cccgagttcc tcgagatcag cccgaacaac aagattcccg cgatcgtcga ccgcgacaac
     1381 ggcatgaagc tgatggagtc ggcggcgatc atgatctatc tcgccgagaa gacggcaagc
     1441 ccgttgtggc cgaccgagat ggagccgcgc ttcgacgtgc tgcaatggct gatgatgcag
     1501 atgggctcgg tcgggcccat gctcggccag acccaccact tcgtgaagtt caatcccggc
     1561 aaggcgccct atgccgagca acgctatcgc aaggagaacg cgcgcatcta tgcggtgctc
     1621 gaccgccagc tcggccggcg gccctatctc gccggcgaca tctcgatcgc cgacatcgcg
     1681 acctggccct ggatctcgcg cttcgagtgg caggagatgg acctcggcgc ctatccgaat
     1741 ctcaagcggt ggtacctcga catcgccgcg cgcccggcga cgcagcgcgg ctacgacgtg
     1801 cccgcacgcg gcttcgaaat ccccatgccg tagccgcgcc ggggcttttt caggccgggt
     1861 gaacagggta cggttggggc gactgcgggt agagacgcgg gacgatcggc gagaatgggc
     1921 gacaagctga ccccgttcga gaccttccgg caactgaagg aacgacagcg ggccgaggaa
     1981 cgggcgcgcg tggagtcggc gcgcggcgag gaggcacgag ccggactgac cgagatcatg
     2041 gccgtcatcg aggataccgg ccgcctggtc ggccgccttc agggcggtct caagcgcgag
     2101 ctggcgcacg cgctggcccg cggcgaggcg tccggggtcg acggcatgcg caacctggtg
     2161 cgccgttacg agcgcctgtc gtgggagctg cacgggcagc tgcgcgcggt gctggaaggg
     2221 gcgcgcaagg gccgctgacg gcgcgaccat ggcccacagg ctgttcgtcg gcgtcgacct
     2281 gccggcgctg gtgcgcgagg cgctgcaccg gctgcaattc ggggtgcggt cggcgcgctg
     2341 ggtggcgccc gagaacctgc acctgacgct ccgcttcatc ggcgacgtcg acggccgcca
     2401 ggcgcaggac atccacgatg cgctgacggc gctcaacgcc cggagcttca ccatcgagct
     2461 gcgcgacgcc ggctgcttcg gtcccggcaa gaggccgcgc gcgctgtggg cgggggtggc
     2521 gccgtcgcgc gagctggacg agctcaagct gcgcgtcgac ggcctgctcg ctcgcgccgg
     2581 ggtgccgccg gattcgcgca ggtactggcc ccacgtcacg cttgcccggc tgcgcaacga
     2641 cgcggcgcgc gagatcggcg cgcgcattgc cgagatctcg ccggccgtcg cgggcgcctt
     2701 cgaggtcgag gagctgatcc tgttccagag ccacaagagc acctcgggca gctattactc
     2761 gcgcgaggcc gcctatccgc tgcgcccatc gtgaccgccg ccggcgcccg cctcgagatg
     2821 ctgctcgaac aggtgcgcgc gtgccggctg tgcgcggcgc accttccgca tggcccgcgc
     2881 ccggtgctga gggcgcggcc gagcgcgcgg ctcctgatcg tcggccaggc gccgggcacc
     2941 cgcgtgcacg agaccgggat tccctggaac gacccgtcgg gcgaccggct gcgccagtgg
     3001 ctcgggctcg atcgcgcccg cttctacgac gaccggacgg tcgccatcgt cccgatcggg
     3061 ctgtgctatc cgggccgcga cgcccatggc ggcgaccggc cgccgcggcc ggaatgcgcg
     3121 ccgctgtggc atccccggct cggcgcggag ctgggcgcgg tcgagctggt gctgctggtc
     3181 ggcggctacg cccagcgcta ctacttggcg gaacgcgcgc ggccgaccgt caccgagacg
     3241 gtgcgcgcct ggcgcgacca tctgccccgc ttcgtgccga ccccgcaccc gtcgtggcgg
     3301 acgcaagcct gggtgcggcg caacccgtgg ttcgagagcg agctgctggc gtacctgcgc
     3361 cggcgcgtgg ccgaccttct cggccccgcc tgacgggctc gccacagggc ctagttgatc
     3421 ttggtcagct ccggcaggtc gtgggccggg tagcaggtcg cggcgatgaa cgagacgcgg
     3481 tagaacgacg atagcgaggc gtggctggcc gggtcgaacg acaggctgcc ggccaaatag
     3541 gtgtgcttgg tggtccggtg caggatcttg ccgacgccgt tgatccgcgg ccccgagcgg
     3601 atgaaggtct cgcgcatcat ggcgagctcg accggctggc cggcgttggc atagggccag
     3661 ttgaccagca agcgcccgcc ggttaagcgg ccgtcgtggt cgtaatgggg cgagagcctg
     3721 ccggtgatct cctgctgggg caggtccagc ggcgtgccga ccttggtcac catgccgccg
     3781 atgcggtcgt c
//
LOCUS       DASWHU010000411         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1081167, whole genome shotgun sequence.
ACCESSION   DASWHU010000411 DASWHU010000000
VERSION     DASWHU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081167"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1082)
                     /gene="thyX"
                     /locus_tag="VGB88_15115"
     CDS             complement(114..1082)
                     /gene="thyX"
                     /locus_tag="VGB88_15115"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9648835.1"
                     /translation="MPPAGEERLSEEERAAVAASRAESRPTRRVSVAALEDILYRPLP
                     VLDHGFVRVIDYMGDDGAVVQAARVSYGTGTRKLREDAGLIRYLMRHRHTTPFEMCEI
                     KLHVKLPIFVARQWIRHRTANVNEYSARYSILDREFYLPAPEHLAAQSTANRQGRGTV
                     LSGPEAERVMALLRADSMRAYDDYVEMLNADEQGNTRDPSRQGLARELARMNLSLNFY
                     TQWYWKIDLLNLLHFLGLRADDHAQYEIRAYAEVILDQVVARWVPLTCAAFRDYVAGA
                     FTLSAPALAVVRRMLAGESVDQAASGLSAREWRELMAALGAATDRA"
     gene            1165..1522
                     /gene="ssrA"
                     /locus_tag="VGB88_15120"
     tmRNA           1165..1522
                     /gene="ssrA"
                     /locus_tag="VGB88_15120"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT          224 a          544 c          544 g          233 t
ORIGIN      
        1 tcgcgccccg acagccagca cgcgcgcacc ttccgcgcca tggccgcgcg ggtgatggag
       61 cggctcgccc ccggcgccga gctgcgccgc ccgccgccca ggatcgtggt cagctaggcc
      121 cggtcagtcg ccgccccgag cgcggccatc agctcgcgcc actcgcgcgc gctcagcccg
      181 ctcgctgcct ggtcgaccga ttcgccggcg agcatgcgcc ggaccaccgc cagcgccggc
      241 gccgacaggg tgaaggcgcc cgccacgtag tcgcggaagg cggcgcaggt gagcggcacc
      301 cagcgcgcca cgacctggtc gagaatcacc tcggcatagg cgcgaatctc gtattgggcg
      361 tggtcgtcgg cgcgcaagcc gaggaagtgc agcaggttga gcaggtctat cttccaatac
      421 cactgggtgt agaagttgag cgacaggttc atgcgcgcga gctcgcgtgc aaggccctgg
      481 cggctgggat cgcgggtgtt gccctgctcg tcggcattga gcatctcgac atagtcgtcg
      541 taggcgcgca tcgagtcggc gcgcagcagc gccatcaccc gctcggcctc gggcccgctg
      601 agcacggtgc cccggccctg gcggttggcg gtcgactggg cggccaggtg ctcgggcgcg
      661 ggcaggtaga actcgcggtc gaggatcgag tagcgcgccg agtactcgtt gacgttggcg
      721 gtgcggtggc ggatccactg gcgggcgacg aagatcggca gcttcacgtg cagcttgatc
      781 tcgcacatct cgaacggcgt cgtgtggcgg tggcgcatga ggtagcggat caggccggcg
      841 tcctcgcgca gcttgcgcgt gccggtgccg taggacacgc gcgccgcttg caccacggcg
      901 ccgtcgtcgc ccatgtagtc gatcacccgc acgaagccgt ggtcgagcac cggcagcggc
      961 cggtacagga tgtcctcgag cgccgccacg ctcacccgcc gggtcggccg gctttcggcg
     1021 cgcgaggccg cgaccgccgc ccgctcctcc tccgacagcc gctcctcgcc cgcgggaggc
     1081 atgtggtcct cgctcccggc taggaacttg ggggcttcgc tggcggccat tcttggtctc
     1141 cctcgctcgg tcgcggggcc ggcggggctt ccgaaacgcc ggccgatcgc ctatatatgt
     1201 gaggtcggcg gtgccgacta tggcgataaa cgctgcgtgg aataagcgtc atggacccgg
     1261 gggcagtacc cggcgcctcc accaatccca gctcggccgc aggccgtacc tctgggggcg
     1321 aaacaggctc gacatgcgcg gtaaagacga agctttcgcc cggcatggta ccaccgttat
     1381 cgggccgaac gcatagttgc caacgacaac tacgcggagg tgcgcctcgc cgcgtaagcg
     1441 gccggtagcc tcaatctaag ccctcggcgg tcgcacgccg aggcggggtt cggggcgcac
     1501 ctggcaacag aagcgcccct cttccgtctc gctccgcccg cgacc
//
LOCUS       DASWHU010000412         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3286903, whole genome shotgun sequence.
ACCESSION   DASWHU010000412 DASWHU010000000
VERSION     DASWHU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3286903"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1443)
                     /locus_tag="VGB88_15125"
     CDS             complement(13..1443)
                     /locus_tag="VGB88_15125"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9648836.1"
                     /translation="MDLSGFERSGDCEWRIAPTGAMRVPAVIYADEALMRDMDDKVRE
                     QVTNVATLPGIVGAAYAMPDAHWGYGFPIGGVAAFDAEAGGVVSAGGVGFDISCGVRA
                     LTTGLARADIEAHQGALADALFRTVPAGLGSTGRITLDDAEMEAMLAGGARWAVARGW
                     GEALDLERIEERGAAEDARPDWVSAHAKQRQRKEMGTLGSGNHYLEVQYVAHIYDQAV
                     ARGFGLALGDAVVSIHCGSRGLGHQVATAYLPQMLDDAHARALALPDRDLACAPIKSD
                     LGARYLGAMRAGINCALANRQIITALARQAFAEVFAEARLELVFDVSHNTCKVERHRV
                     DGAERTLHIHRKGATRAFGPGHPSLPESLRAHGQPVLIGGSMGTESYVLVGVPEGEAH
                     AFSSACHGAGRAMSRHAAAKRWRGRELIDSLKAAGILIRSPSARGVAEEAPGAYKDAG
                     AVCEAAERAGLARRVARLKPLVCVKG"
     gene            complement(1484..1906)
                     /locus_tag="VGB88_15130"
     CDS             complement(1484..1906)
                     /locus_tag="VGB88_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX9648837.1"
                     /translation="MGEAGWQHFEHGSDVGVRGFGRTRAAAFAEAARALTAAVAELDS
                     VRPETPVEISCAAPDDERLLVAWLNAVVYEMATRTMLFARFEVAIDDGRLTATAWGEP
                     VDVARHQPAAEVKGATYTALSVRRRADGGWIAECVVDV"
     gene            complement(1949..>2248)
                     /locus_tag="VGB88_15135"
     CDS             complement(1949..>2248)
                     /locus_tag="VGB88_15135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9648838.1"
                     /translation="VRHYAAADVFVFPSRTDTFGLVLLEALACGVPVAAFPVPGPIDV
                     VDGSAVGVLDDDLAQACERAIAIPRARCRDFALTFSWSAVADQFLANLAPLPGRG"
BASE COUNT          295 a          819 c          849 g          285 t
ORIGIN      
        1 gcggcttgcg cctcatccct tcacgcacac cagcggcttg aggcgggcga cgcggcgggc
       61 gaggccggcg cgttcggccg cttcgcacac cgcgcccgcg tccttgtagg cgccgggggc
      121 ctcctcggcg acgccgcgcg ccgacgggct gcggatcagg atgccggcgg ccttgagcga
      181 gtcgatcagc tcgcgcccgc gccagcgctt ggcggcggca tgccggctca tcgcccgccc
      241 ggcgccgtgg caggccgaag aaaacgcgtg cgcctcgccc tcgggcacgc cgaccagaac
      301 gtaggactcg gtgcccatcg agccgccgat cagcaccggc tggccgtggg cgcgcaagct
      361 ctcgggcagg ctgggatggc cggggccgaa ggcgcgggtc gcgcccttgc ggtggatgtg
      421 gagcgtgcgc tccgcgccgt ccacccggtg gcgctcgacc ttgcaggtgt tgtgcgagac
      481 gtcgaacacc agctcgaggc gcgcctcggc gaacacctcg gcgaacgcct ggcgggcgag
      541 cgcggtgatg atctggcggt tggcgagcgc gcagttgatg ccggcgcgca tggcgcccag
      601 atagcgcgcg cccaagtcgg acttgatcgg tgcgcaggcg aggtcgcggt cgggcagcgc
      661 gagtgcgcgc gcgtgcgcgt cgtcgagcat ctgcggcagg taggcggtgg cgacctggtg
      721 gccgaggccg cgcgagccgc agtggatgct gaccaccgcg tcgccgaggg cgaggccgaa
      781 gccgcgcgcc accgcctggt cgtagatgtg ggcgacgtac tggacctcga gatagtggtt
      841 gcccgagccg agcgtgccca tctccttgcg ctggcgctgc ttggcgtggg ccgagaccca
      901 gtccgggcgg gcgtcctcgg cggcgccgcg ctcctcgatc cgctcgaggt cgagcgcctc
      961 gccccagccg cgcgcgaccg cccagcgtgc gccgccggcg agcatcgcct ccatctcggc
     1021 gtcgtcgagc gttatgcggc cggtgctgcc caagcccgcg gggacggtgc ggaacagggc
     1081 gtcggcgagc gcgccctgat gggcctcgat gtcggcgcgg gcaaggcctg tggtcagcgc
     1141 gcgtaccccg cacgagatgt cgaagccgac cccgccagcg gagaccacgc cgcccgcctc
     1201 ggcgtcgaac gcggccaccc cgccgatcgg gaagccgtag ccccaatggg cgtcgggcat
     1261 ggcgtaggcg gcgccgacga ttcccggcaa ggtggcgacg ttggtcacct gctcgcgcac
     1321 cttgtcgtcc atgtcgcgca tcagcgcctc gtcggcgtaa atcaccgcgg gcacgcgcat
     1381 cgcgccggtc ggcgcgatcc gccactcgca gtcgccgctg cgctcgaagc cgctcaggtc
     1441 catctcggcg ccgtcctttc gcgcgacata tggggagcgg ccgctacacg tccactacgc
     1501 actcggcgat ccagccgccg tcggcccggc ggcggacgct gagcgcggtg taggtcgcac
     1561 ccttcacttc cgcggccggc tggtggcggg ctacatcgac cggctcgccc caggcggtgg
     1621 cggtgagccg gccgtcgtcg atcgcgacct cgaagcgcgc gaacagcatg gtgcgggtgg
     1681 ccatctcgta gaccaccgca ttgagccagg cgaccagcag gcgctcgtcg tcgggcgcgg
     1741 cgcacgaaat ctcgaccggc gtctcgggcc gcaccgagtc gagctcggcg accgccgcgg
     1801 tgagcgcccg cgcggcctcg gcgaacgccg ccgcgcgcgt acgcccgaag ccgcgcacgc
     1861 cgacgtcgga gccgtgctcg aagtgctgcc agccggcctc gcccatggcg cgacagcttg
     1921 gccgcgcgcg tgccctccgg caagcgcttc agccgcggcc gggcagcgga gcgaggttgg
     1981 cgaggaactg gtcggcgacc gccgaccacg agaaggtgag cgcgaagtcg cggcagcgcg
     2041 cgcgcgggat ggcgatggcg cgttcgcacg cttgggccag gtcgtcgtcg agcacgccca
     2101 ccgcgctgcc gtcgaccacg tcgatcggac ccggcaccgg gaacgccgcc accggcacgc
     2161 cgcaggcgag cgcctcgagc agcaccaggc cgaaggtgtc ggtgcggctc gggaacacga
     2221 acacgtcggc ggcggcgtag tggcgcac
//
LOCUS       DASWHU010000413         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2548878, whole genome shotgun sequence.
ACCESSION   DASWHU010000413 DASWHU010000000
VERSION     DASWHU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548878"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /locus_tag="VGB88_15140"
     CDS             <1..>1598
                     /locus_tag="VGB88_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9648839.1"
                     /translation="QDAIEDEVAYLVNAAGCRLVLAEDEEQVDKLLALGARAPSLARI
                     AYNDPRGMRKYDDARLVALEDVMRRGEARHRAEPALYDQEVAKGCGGDVAMLITTSGT
                     TSNPKLAMMQAGPFLGHCAAYLRADPKRPGDDYVSVLPLPWIMEQVYAVAQPLLCRAC
                     VNFVEEPATAMADLREVGPSFVLFAPRVWEQVAADVRARVMDASWLKRALFDLGTRLG
                     ARALAAGGRSWLADAILYRALRDRLGFSRLRSAATGGAAMGPDTFRFFLAMGVPLRQL
                     YGQTELTGAYTIHSPGDVDFDTVGRPFDNTELRIDRADASGVGEICTRHGGMFAGYWR
                     DQAATAQVLDQDGWMHTGDAGYFDAKRHLVVIDRVKDLATTAAGARFSPQFIENKLKF
                     SPFIGEAVVLGDRRPHLAAILCIRHSIVSKWAERRRIGFTTYTDLASRAEVTELIRGE
                     IERVNEILPEAQRIRRFLLLYKELDADDGELTRTRKVRREVVGRRYGELIEAMYAGRD
                     RVHVDTTITFQDGTTQRVRTDIAIV"
BASE COUNT          234 a          576 c          552 g          236 t
ORIGIN      
        1 caggacgcga tcgaggacga ggtcgcctat ctggtcaatg ccgccggctg ccggctggtg
       61 ctggccgagg acgaggagca ggtcgacaag ctgctggcgc tgggcgcgcg cgcgccgagc
      121 ctcgcccgca tcgcctacaa cgatccccgc ggcatgcgaa agtatgacga tgcgcgcctg
      181 gtcgcgctcg aagacgtgat gcgccgcggc gaggcgcgcc accgcgccga gcccgcgctc
      241 tacgaccagg aggtggcgaa gggctgcggc ggcgacgtcg ccatgctcat caccacctcg
      301 ggcaccacct cgaacccgaa gctcgcgatg atgcaggcgg ggccgttcct cggccactgc
      361 gcggcgtacc tgcgcgccga ccccaagcgg ccaggcgacg attacgtctc ggtgctgccg
      421 ttgccgtgga tcatggagca ggtctacgcg gtggcgcagc cgctcttgtg ccgggcgtgc
      481 gtcaatttcg tcgaggagcc ggcgacggcg atggcggatt tgcgcgaggt cggcccgtcc
      541 ttcgtcctgt tcgcgccgcg ggtgtgggag caggtcgcgg ccgacgtgcg cgcgcgggtg
      601 atggacgcgt cctggctcaa gcgcgcactg ttcgatctcg gcacgcggct cggcgcacgc
      661 gcgctcgccg cgggcgggcg ctcgtggctt gcggacgcga ttctttaccg cgcgctgcgt
      721 gaccggctcg gcttctccag gctgcgctcg gcggcgaccg gcggcgccgc gatggggccc
      781 gacacgttcc gcttcttcct cgccatgggc gtgccgctcc gccagctcta cggccagacc
      841 gagctcaccg gcgcctacac catccacagc ccgggcgatg tcgacttcga caccgtcggg
      901 cggccgttcg acaacaccga gctgcgcatc gaccgcgcgg acgcgagcgg cgtcggcgag
      961 atctgcaccc gccatggcgg catgttcgcg ggctactggc gcgaccaggc ggcgaccgcc
     1021 caggtgctcg atcaggacgg ctggatgcac accggggatg ccggctattt cgacgctaag
     1081 cgccatctgg tggtcatcga ccgggtcaag gacctcgcca ccaccgccgc cggcgcccgc
     1141 ttctcgcccc agttcatcga gaacaagctc aagttctcgc cgttcatcgg cgaggcggtg
     1201 gtgctcggcg accgccgccc gcacctcgcc gcgatcctgt gcatccgcca ctcgatcgtc
     1261 tccaaatggg cggagcgcag gcgcatcggt ttcaccacct acaccgacct cgcgagccgc
     1321 gcggaagtca ccgagctgat ccggggcgag atcgagcggg tcaatgagat cctgcccgag
     1381 gcgcaacgca tccggcgctt cctgctcttg tacaaggagc tcgacgccga cgacggcgag
     1441 ctcacgcgca cgcgcaaggt ccgccgcgag gtggtcgggc gccgctacgg cgagctgatc
     1501 gaggcgatgt acgccggccg cgaccgggtc cacgtcgata ccaccatcac cttccaggac
     1561 ggcaccaccc agcgcgtgcg gaccgacatc gccatcgt
//
LOCUS       DASWHU010000414         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_693967, whole genome shotgun sequence.
ACCESSION   DASWHU010000414 DASWHU010000000
VERSION     DASWHU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693967"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGB88_15145"
     CDS             complement(<1..288)
                     /locus_tag="VGB88_15145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9648840.1"
                     /translation="MLVHRITRTVQWGDCDPMGIVFYPNFYRWMDEASWAMYAAHEIT
                     LPVMEERFGVVGTPLVETGCAFKSPARQGDELLVETQVLSWSTRSFRVGHAF"
     gene            complement(304..1752)
                     /locus_tag="VGB88_15150"
     CDS             complement(304..1752)
                     /locus_tag="VGB88_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9648841.1"
                     /translation="MNAADVVLAAGADEAPALITETETVGYGALRRFVGRAGNGLAAA
                     GVRPGERVVLVLDDRPLLVAAYLGAIRIGAVPVAVNTRWSEDEIGFAIEDTGARLALV
                     EPHYGAARHAAGGRALIATDGPFDDRPDTLKAWDAEPDDPALMIYTSGSTGRPKAAVH
                     RHRAVRIAARTMAEVFGLGAKDRVLITSKMFFAYALGHGLLGALKLGAAVVLDTRWPK
                     PAEVAELAARTGTTAFYSVPTFYRALLDVNPAAEGLGTVRRYVSAGERLPPGLFARWR
                     EATGAAIADGIGTSETLFMFLANPPEAPRPGSAGVPASGAEVRLKTGDGDNDGETGVL
                     WVRLESLAQGYWRRPAETARAFRDGWFRTGDLFRRDEDGFWHHQGRADDMIRVAGQWL
                     DPGEVEDCALATGACTEAALVGVPDADGLNRVVLFVAGATDANAAERIADNLARRLAP
                     YKRPAEIRIVAALPRTATGKVQRHVLRDTGTG"
     gene            complement(1749..3428)
                     /locus_tag="VGB88_15155"
     CDS             complement(1749..3428)
                     /locus_tag="VGB88_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9648842.1"
                     /translation="MYDLLIKDARIHDGLGAEPVAGDVAVAGGRIVDIGRISAVAAET
                     VDAQGLALMPGIVDVHTHYDAQLTWEPTASPSPALGVTTVVVGNCGFGIAPCPAEARE
                     AVVRNLSEVEGMPLEVLEGGIDWSFTTFADYLALLRRKGTTPNVGVFAGHSTIRSVVM
                     GPAASERAATAEEIARMRAEVAAALAAGAIGFASSQSLNHMGYGGVPMPSRLAEEAET
                     RALVGVLGAAAKGLFQITVGPATTVASLEELARDTRRPVVFSALFHNDAYPERAPSML
                     AQCNQARARGAEVYAQVGCQPLSMDFTLANAYPFQSLDIWAPLRAAAPEAIARAIADR
                     DFRVRFRAAIGARRKGTLFYGDWHRVEIAEAATGANAALEGLDLAEVAARQRKDPLDA
                     FFDLALAEELRTVFNAKLLNTDEDIVAGLLRDPSSLISLSDAGAHLTYLCDAGYGLHL
                     LGHWTRARATFTLPEAVRELTSRPADLYGIAGRGRIAPGAQADLLLFDPDTVEVSRPR
                     RVFDLPGGSGRLVRDGIGVHGVWVNGAQVFDGSDYVAQQRPPGTVLDRFGP"
BASE COUNT          504 a         1274 c         1277 g          453 t
ORIGIN      
        1 gaacgcatgc ccgacccgga acgagcgcgt cgaccacgac agcacctgcg tctcgaccag
       61 cagctcgtcg ccctggcgcg ccgggctctt gaacgcgcag ccggtctcga ccagcggcgt
      121 gccgaccacg ccgaagcgct cctccatcac cggcagggta atctcgtgcg cggcgtacat
      181 cgcccacgac gcctcgtcca tccagcggta gaagttgggg tagaacacga tccccatcgg
      241 gtcgcagtcg ccccactgga cggtgcgcgt gatgcggtgg acgagcacgg cgccgcgcgg
      301 ggctcagccg gtgccggtgt cgcgcaagac atggcgctgc accttgccgg tcgcggtacg
      361 cggcagcgcc gcgacgatcc tgatctcggc cggtcgcttg tagggcgcga gccggcgggc
      421 gagattatcg gcgatgcgct cggccgcgtt cgcatccgtc gcaccggcaa cgaatagcac
      481 gacacgattc aggccgtccg cgtctggcac gccgaccaga gccgcctcgg tgcaggcacc
      541 ggtcgcgagc gcgcagtcct cgacctcgcc cgggtcgagc cattggccgg cgaccctgat
      601 catgtcgtcg gcgcggccct ggtggtgcca gaagccgtcc tcgtcgcgac ggaacaggtc
      661 gccggtgcgg aaccagccgt cgcggaacgc gcgcgcggtc tcggccgggc gccgccaata
      721 gccttgggcg agagactcga ggcgcaccca cagcacgccg gtctcgccgt cgttgtcgcc
      781 gtcgccggtc ttcaggcgca cctcggcgcc cgaggccggc accccggcgc tgcccggccg
      841 cggcgcttcg ggcggattgg caaggaacat gaacagcgtc tccgaggtgc cgatgccgtc
      901 cgcgatcgcc gcaccggtcg cctcccgcca gcgcgcgaac aggcccggcg gcaggcgctc
      961 gccggccgag acgtagcggc gcacggtgcc gaggccctcc gctgccgggt tcacgtccaa
     1021 cagcgcgcgg tagaaggttg gcacgctata gaatgcggtc gtgccggtgc gcgccgcgag
     1081 ctcagccacc tccgccggct tcggccagcg cgtatccagc accacagcgg cgccgagctt
     1141 gagcgcgccg aggagcccgt ggccgagcgc gtaggcgaag aacatcttgg acgtgattaa
     1201 cacccggtct tttgcgccga gcccgaacac ctcggccatg gtccgggcgg cgatccggac
     1261 cgcgcgatgg cggtggaccg ccgccttggg ccggccggtg ctgcccgagg tgtagatcat
     1321 gagcgccgga tcgtcaggct ccgcgtccca cgccttgagc gtatcggggc ggtcgtcgaa
     1381 cggtccgtcg gtggcgatca gcgcgcgccc gcccgcggcg tgccgcgccg cgccgtagtg
     1441 cggctcgacg agggcgagcc gcgcgcccgt gtcctcgatg gcgaagccga tctcgtcctc
     1501 ggaccagcgc gtgttgaccg ccaccggcac ggcgccgatg cggatcgcgc cgagataggc
     1561 cgcgaccagc agcggccggt cgtcgagcac cagcacgacc cgctcgccgg gacggacccc
     1621 ggccgcggca aggccgttgc cggcgcgccc gacaaagcgc ctgagcgcgc cgtagccgac
     1681 ggtctcggtc tccgtgatca gcgccggggc ttcgtccgcg ccggcggcga gcaccacgtc
     1741 ggcggcgttc atggcccgaa gcggtcgagc accgtgcccg gcgggcgctg ctgcgccacg
     1801 tagtcgctgc cgtcgaacac ctgcgcgcca ttgacccaca ccccgtgcac gccgatcccg
     1861 tcgcgcacca ggcgcccgct gccgcccggc aggtcgaaca cccgcctggg ccgcgacacc
     1921 tcgaccgtgt cgggatcgaa cagcagcagg tcggcctgcg cccccggcgc gatgcggccg
     1981 cgcccggcga tgccgtagag gtcggccggg cggctggtca gctcgcgcac cgcctcgggc
     2041 agggtgaacg tggcgcgcgc gcgggtccag tggccgagca gatgcaggcc gtagccggca
     2101 tcgcacagat aggtgaggtg cgcgcccgcg tccgacagcg agatcaggct cgacggatcg
     2161 cgcagcaggc cggcgacgat gtcctcgtcg gtgttgagca gcttggcgtt gaacacggtg
     2221 cgaagttcct cggcaagggc gaggtcgaag aacgcatcga gcgggtcctt gcgctggcgc
     2281 gcggcgacct cggccaagtc gaggccttcc agcgccgcgt tggcgccggt cgcggcctca
     2341 gcgatctcga cccggtgcca gtcgccgtag aacaaggtgc ccttgcggcg tgcgccgatc
     2401 gcggcgcgga agcggacacg gaagtcgcgg tcggcaatgg cgcgcgcgat ggcctcgggc
     2461 gcggcggcgc gcaacggcgc ccagatgtcg aggctctgga acggataggc gttggcgagg
     2521 gtgaagtcca tgctcagcgg ctggcagccg acctgcgcgt agacctcggc gccacgcgcg
     2581 cgcgcctggt tgcactgagc caacatcgac ggcgcgcgct caggatacgc gtcgttgtgg
     2641 aacagcgccg agaacaccac cggccgccgg gtgtcgcgcg cgagctcctc cagggacgca
     2701 accgtcgtcg ccgggccgac ggtgatctgg aacagcccct tggccgccgc gccgagcacg
     2761 ccgacgagcg cccgcgtctc tgcctcctcg gcgagccgcg agggcatcgg cacgccgcca
     2821 tagcccatgt ggttgagcga ctgcgaggag gcgaagccga tcgcgcccgc agcgagcgcg
     2881 gcggcgacct cggcgcgcat gcgcgcgatc tcctcggcgg tcgccgcgcg ctcggacgcc
     2941 gccggcccca tcaccacgct gcggatggtc gagtggccgg cgaacacgcc gacattggga
     3001 gtggtgccct tgcgccggag cagcgccaga tagtcggcga aggtagtgaa gctccagtcg
     3061 atgccgccct cgagcacctc gagcggcatg ccctcgacct ccgacaggtt gcgcaccacc
     3121 gcctcgcgcg cctcggccgg gcagggggcg atgccgaagc cgcagttgcc gaccaccacc
     3181 gtggtcacgc cgagcgcggg cgagggcgag gcggtcggct cccaggtgag ctgggcgtcg
     3241 taatgggtgt gcacgtcgac gatgccgggc atcagcgcga gcccttgggc gtccaccgtc
     3301 tcggcagcca cggcggagat gcggccgatg tcgacgatcc gcccgcccgc gaccgcgacg
     3361 tcgcccgcga ccggctcggc cccgagcccg tcatggatgc gcgcgtcctt gatcaagagg
     3421 tcgtacatgg tgtcccttcc gccgcggccg gcagagttta ggcgaaagcg ccttgcgggc
     3481 aagggcggag ccggtgcaag ctctggcg
//
LOCUS       DASWHU010000415         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_1085890, whole genome shotgun sequence.
ACCESSION   DASWHU010000415 DASWHU010000000
VERSION     DASWHU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085890"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGB88_15160"
     CDS             complement(<1..222)
                     /locus_tag="VGB88_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648843.1"
                     /translation="MDQPFEKWAPPEIARLRELADSLERALGLYAAAHESGQPERHRV
                     ERVRLPQRRGGRRPGVKTQAIVGHINDHAD"
     gene            391..813
                     /locus_tag="VGB88_15165"
     CDS             391..813
                     /locus_tag="VGB88_15165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9648844.1"
                     /translation="MQHLLPAGWKRPSGYSAGIAADGHLVFVAGMIGWNAAEEFETDD
                     LVGQLRQALVNIRAVLAAAGAGPQHVARMTWYLTDRRAYLDRREEIGHVWREVMGRVY
                     PAMAVVEVSALLEDRAKVEIETTAVVPRDEPLFSSWAP"
     gene            895..1431
                     /locus_tag="VGB88_15170"
     CDS             895..1431
                     /locus_tag="VGB88_15170"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX9648845.1"
                     /translation="MIRTAIVLAGLAVPLTGCAGGRWQDTLTAEDQALMTQGVQGALE
                     TGRTGEGANWTNPATGNRGTVMPTGTFKNYEGLDCRDYQQTVTIAGDTRVGWGTACRH
                     ANGVWVDRRSPALRPAAQVASAAEYRARPRLHFGFGYGTGFGHPYRGWYQGYRFGPYP
                     WYGYGGPFYDPFYDPFYW"
     gene            1454..2182
                     /locus_tag="VGB88_15175"
     CDS             1454..2182
                     /locus_tag="VGB88_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9648846.1"
                     /translation="MPAAVHIGTSGWHFPHWVGPFYPRDLPPEEFLGFYARHFRTVEI
                     NASFYRLPERTTVAGWRATVGPGFVFAVKASRYATHMKKLKEPETSLARFFDVMAALG
                     PALGPVLFQLPPRFRVNLARLDACLGALPRGVRAAFEFRDRSWFTDAVYDSLAAHGAA
                     LCVYDLAGWRAPLAATADFVYLRLHGPHAAYQGRYGRARLRPWARRIAAWRAEGREVF
                     CYFDNDQAGYAVTDALSLTALVSP"
     gene            complement(2201..>2378)
                     /locus_tag="VGB88_15180"
     CDS             complement(2201..>2378)
                     /locus_tag="VGB88_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9648847.1"
                     /translation="TLTSCPAMSVPCGFTADGRPVGVQMVAPPRAEDRLLAAAAAFEG
                     AHGYARMVPIDPRR"
BASE COUNT          333 a          866 c          820 g          359 t
ORIGIN      
        1 atcggcatga tcgttgatat gccccacgat cgcctgcgtc ttgacgcctg gccggcgccc
       61 gccgcgacgt tgaggcaggc gaactctctc gacgcggtgc cgctctggct gcccgctttc
      121 atgtgccgca gcgtacaacc caagtgcccg ctccaaggaa tccgccagct cgcgcaatcg
      181 tgcgatttcc ggcggcgccc acttttcaaa cggctggtcc atgccaagcc ctccgcagag
      241 aatcgacgcg gaacatatgg ttgcggctgg caggccgtca agacgtaatt ctctgcccaa
      301 gccccttccg gcgggccggc gaagtcaacc ggagagaaat tcaaactgag gcactaccgg
      361 ccgatccgcc caacgcgaac tggaggcagc atgcagcatc tcttgccggc cggatggaag
      421 cggccgtccg ggtacagcgc cggcattgcc gccgacgggc acctggtctt cgtcgccggc
      481 atgatcggct ggaacgcggc cgaggagttc gagaccgacg acctggtcgg ccagctccgc
      541 caggccctgg tcaacatccg cgccgtgctc gccgcggccg gcgccgggcc ccagcacgtc
      601 gcgcgcatga cctggtacct gaccgacagg cgcgcctatc tcgaccgccg ggaagagatc
      661 gggcacgtct ggcgcgaggt gatgggtcgg gtctatccgg cgatggcggt ggtcgaggtc
      721 agcgcgctgc tcgaggaccg cgccaaggtc gagatcgaga ccaccgccgt ggtgccgcgc
      781 gatgagccgc tgtttagctc gtgggcgccg tgaccctttg cgcgccggcg gcggcaatgg
      841 cataagtgtc atgatgagcg tcggggcacc gcagcgacaa ggaggccccg agccatgatc
      901 cggaccgcga tcgtcctcgc cggcctggcc gtgccgctca ccggctgcgc cggcggccgc
      961 tggcaggata cgctgaccgc cgaggaccag gcgttgatga cgcagggcgt ccagggcgcg
     1021 ctcgagaccg ggcgcaccgg cgaaggcgcc aactggacca acccggcgac cggcaaccgc
     1081 ggcacggtca tgccgaccgg cacgttcaag aactacgaag ggctcgactg ccgcgactac
     1141 cagcagacgg tgaccatcgc cggcgacacc cgcgtcggct ggggcaccgc ctgccggcac
     1201 gccaacggcg tctgggtcga ccggcgctcg ccggccttga ggcccgcggc gcaggtcgcg
     1261 agcgccgccg agtatcgcgc gcgcccgcgc cttcatttcg gcttcggcta tggcaccggt
     1321 ttcggccatc cctatcgcgg ctggtaccag ggttatcggt tcgggccgta tccctggtac
     1381 ggctacggcg gcccgttcta cgacccgttc tacgatccct tctactggta ggcggcagcg
     1441 ccgcggcgcc ggcgtgcccg ccgcggtcca tatcggcacc tcgggctggc actttccgca
     1501 ctgggtaggg ccgttctacc cgcgcgatct tccgcccgag gagttcctcg gcttttacgc
     1561 gcgccacttc cgcacggtcg agatcaacgc cagcttctac cgcctgcccg agcggacaac
     1621 cgtcgccggg tggcgcgcga ccgtcggtcc cggtttcgtg ttcgcggtca aggcgagccg
     1681 ctacgccacc cacatgaaga agctcaagga gccggagacg tcgctggcgc ggttcttcga
     1741 tgtgatggcg gcgctcggcc cggcgctcgg gccggtgctg ttccagctgc cgccgcgctt
     1801 ccgggtgaac cttgcccggc tcgacgcctg tctcggcgcg ctgccgcggg gtgtgcgcgc
     1861 ggcgttcgag ttccgcgacc gcagctggtt caccgatgcg gtctacgact cgctcgcggc
     1921 ccacggcgcc gcgctgtgcg tctacgacct cgccggctgg cgggcgccgc tcgcggccac
     1981 cgccgacttc gtctacctgc gcctgcacgg gccgcacgcc gcctatcagg ggcgctacgg
     2041 ccgcgcccgg ctcaggccct gggcgcgccg catcgcggcc tggcgcgcgg aggggcgcga
     2101 ggtgttctgc tacttcgaca acgaccaggc gggttacgcc gtaacggatg cgctgtcgct
     2161 caccgccctg gtctcaccct gacccggccc gcgaccgggc ctagcgccgc gggtcgatcg
     2221 gcaccatccg cgcgtaaccg tgtgcgccct cgaacgccgc ggcggcggcg agcaggcggt
     2281 cctcggcgcg cggcggcgcg accatctgca cgccgaccgg gcggccgtcg gcggtgaagc
     2341 cgcacggcac cgacatcgcc gggcacgagg tgagcgtg
//
LOCUS       DASWHU010000416         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3627709, whole genome shotgun sequence.
ACCESSION   DASWHU010000416 DASWHU010000000
VERSION     DASWHU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627709"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /gene="glcF"
                     /locus_tag="VGB88_15185"
     CDS             complement(<1..1242)
                     /gene="glcF"
                     /locus_tag="VGB88_15185"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX9648848.1"
                     /translation="MQTGFSLAQLADADISESEKILRACVHCGFCLATCPTYVLLGDE
                     LDSPRGRIYLIKDMLENERAASARVALHIDRCLSCLACMTTCPSGVHYMHLVDHARQR
                     IEETYARPIAERMLRRLLALVLPRPGLFRLALIGARLARPLVGLLPGRLKGMVALAPR
                     RLPKPGMVRGAQVFPAEGARRKRVALLTGCAQVVLAPQINDATVRVLTRHGCEVVVAD
                     GGGCCGALTHHMGRDRDAMRSVKANITAWQREIEGDGLDAIVVNASGCGTMVKDYGFL
                     LRSDPDWAAPAQAIAERARDVSELLDELGLRAGAAPEKLRVAYQSACSLQHGQQVTTQ
                     PKRLLAACGFEVVEPAEAHLCCGSAGTYNLLQPDLAQALRERKLANLEATRADVIASG
                     NIGCITQLGRDAPAPLVHTVEL"
     gene            complement(1254..2501)
                     /locus_tag="VGB88_15190"
     CDS             complement(1254..2501)
                     /locus_tag="VGB88_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9648849.1"
                     /translation="MADDALMAGPFRPTDDAQVREVVAWAAARAMPLEVVGAGTKRGL
                     GRPGNAAAGLDLSRFRGIGWYEAAELGLAAAPGTPVAEITAALAAARQQLAFEPPDLG
                     PLLGAGAGQATIGGILACNLSGPRRIKAGAARDHFLGLHAVSGRAESFKSGGRVVKNV
                     SGYDLCKLLAGSYGTLAVLTDITLKVLPAPEKTRTVLVFGLDDGAAVRALAGAAASSL
                     EPSGLAHLPAAVARASGVDYVSGAGAAVTAVRVEGTEASALTRCTALRELLGAFGAIE
                     ELHGRNSGALWREIADVAAFVAPRGRVVWRLSLPPAQAPAVVADIARALDAAWYYDWA
                     GGLVWLAAPADGDGGANAVRGAIARIGAGGHATLVRADAELRARIPVFQPQAPALAAL
                     ARRIKQQFDPEGVLNPGRMAAEA"
     gene            complement(2639..>3703)
                     /locus_tag="VGB88_15195"
     CDS             complement(2639..>3703)
                     /locus_tag="VGB88_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9648850.1"
                     /translation="PDPSSQIACSIGGNVAENSGGVHCLKYGVTTNNILGLELVLMDG
                     EVVRLGGRHVDSEGYDLLGLLTGSEGLLGVITEVTVRLLPKPETARALLIGFPSVVAG
                     GRCVAEVIGAGIIPGGMEMMDRAAVHAAEAFCHAGYPLDVESLLIVELDGPAVEVDDL
                     AARVQGVAESCGAVTVRTSRSEDERLSFWAGRKNAFPAVGRISPDYYCMDGTIPRARL
                     PEVLARMAELSERFGLRVANVFHAGDGNLHPLILYDANQPDELERAEAFGAEILKLCV
                     EVGGVLSGEHGIGVEKRDLMGVMFDQVDLDQQQRVKCAFDPDGLLNPGKVFPELHRCA
                     ELGRMHIHHGRLPFPDLPRF"
BASE COUNT          528 a         1320 c         1385 g          470 t
ORIGIN      
        1 gagctcgacc gtgtgcacca gcggcgccgg cgcgtcgcgg ccgagctggg tgatgcagcc
       61 gatgttgccg ctcgcgatca cgtccgcgcg cgtcgcctcc aggttggcga gcttgcgttc
      121 gcgcaaggcc tgcgcgaggt cgggctggag caggttgtag gtgccggccg agccgcagca
      181 gagatgcgct tccgccggct cgaccacctc gaagccgcag gcggcgagca ggcgcttcgg
      241 ctgggtggtg acctgctggc cgtgctggag cgagcacgcc gactggtagg cgacccgcag
      301 cttttccggt gcggcaccgg cgcggagccc gagctcgtcg agcagctcgc tcacgtcgcg
      361 cgcgcgctcc gcgattgctt gcgccggcgc cgcccagtcc gggtcgctgc gcaacaggaa
      421 gccgtagtcc ttgaccatgg tgccgcagcc cgaggcgttg accacgatcg cgtccaggcc
      481 gtcgccctcg atctcgcgtt gccaggcggt gatgttggcc ttgacgctcc gcatcgcgtc
      541 gcggtcgcgg cccatgtggt gggtgagcgc gccgcagcag cccccgccgt ccgccaccac
      601 cacctcgcag ccgtggcggg tgagcacgcg cacggtggcg tcgttgatct gcggcgcgag
      661 cacgacttgc gcgcagccgg tgagcagcgc gacgcgcttc cgccgcgcgc cctcggccgg
      721 gaaaacctgc gccccgcgga ccatgcccgg cttcggcagg cgccggggcg cgagcgcgac
      781 catgcccttg agccggccgg gcagcagccc cacgagcggg cgggcgagcc gcgccccgat
      841 cagcgccagg cggaacagtc cgggccgcgg cagcaccagc gcaagcagcc ggcgcagcat
      901 ccgttcggcg atcgggcgcg cataggtctc ctcgatgcgc tggcgggcgt ggtcgacgag
      961 atgcatgtaa tggacgcccg aggggcaggt ggtcatgcag gcgaggcacg acaggcagcg
     1021 gtcgatgtgc agcgccacgc gcgcgctcgc tgcgcgctcg ttctcgagca tgtccttgat
     1081 caggtagatg cgcccgcgcg ggctgtcgag ctcgtcgccg agcagcacgt aggtcgggca
     1141 ggtggcgagg cagaagccgc agtggacgca ggcgcgcagg atcttttccg actcggaaat
     1201 gtccgcatcc gcaagctggg cgagggagaa gccggtctgc atggcggcgc gtctcaggcc
     1261 tcggcggcca tgcggccggg gttgagcacg ccctcagggt cgaactgctg cttgatgcgg
     1321 cgggcgagcg cggcgagcgc cggggcttgc ggctggaaca cggggatgcg ggcgcgcagc
     1381 tcggcgtcgg cgcggaccag ggtcgcgtgc ccgccggcgc cgatgcgcgc gatcgcgccg
     1441 cgcaccgcgt ttgcgccgcc gtcgccgtcg gccggcgccg cgagccagac caggccgccc
     1501 gcccagtcgt agtaccaggc ggcatccagg gcgcgcgcga tgtcggcgac caccgccggc
     1561 gcctgggcgg gcggcagcga cagccgccag acgacgcgcc cgcgcggggc gacgaacgcg
     1621 gcgacgtcgg cgatctcgcg ccacaatgcg cccgagttgc ggccgtgcag ctcctcgatg
     1681 gcgccaaacg cgccgagcag ctcgcgcaag gccgtgcagc gggtgagcgc agaggcctcg
     1741 gtgccctcga cgcgcaccgc ggtgaccgcg gcgcccgcgc ccgacacgta atcgacaccc
     1801 gaggcgcgcg ccacagccgc gggcagatgg gcaaggccgc tcggctcgag gctggacgcg
     1861 gcggcgccgg cgagcgccct caccgcggcg ccatcgtcga ggccgaagac cagcacggtg
     1921 cgcgtcttct cgggcgcggg cagaaccttg agcgtgatat cggtgagcac ggcaagagtg
     1981 ccataagagc ccgcaagcag cttgcacagg tcgtagccgg agacgttctt gaccacgcgc
     2041 ccgcccgact tgaacgattc ggcgcggccc gagaccgcgt gcaggccgag gaaatggtcg
     2101 cgcgccgcgc ccgccttgat ccgtctcggg ccggacaggt tgcaggcgag gatgccgccg
     2161 atggtcgcct gcccggcgcc ggcgccgagc aacggcccga ggtcgggcgg ctcgaaggcg
     2221 agctgctggc gggcggcggc gagcgccgca gtgatctcgg cgaccggggt gccgggcgcg
     2281 gcggcgaggc cgagctcggc ggcctcgtac cagccgatgc cgcggaagcg cgacaaatcg
     2341 agcccggcgg cggcattgcc cgggcgcccg agcccgcgct tggtgccggc gccgaccacc
     2401 tcgagcggca tcgcccgcgc cgccgcccag gcgaccacct cgcgcacctg ggcgtcatcg
     2461 gtcgggcgga acggcccggc catcagcgcg tcgtccgcca agacgggctt cccccctcac
     2521 ccttccctct ccccgcgcgt cgcggcggga gagggttgcg cagggttggc gcggcgaaag
     2581 ccgcgcctta cccggagctg ggtgaggggg gactgcgtca aagcaaccgg accaaacgct
     2641 agaaccgcgg caggtcgggg aatggaagcc tgccgtgatg gatgtgcatg cggccgagct
     2701 cggcgcagcg gtggagctcg gggaacacct tgcccgggtt caacaggccg tcggggtcga
     2761 acgcgcactt gacccgctgc tgctggtcga ggtcgacctg gtcgaacatg actcccatca
     2821 ggtcgcgctt ctcgaccccg atgccgtgct cgccggacag cacgccgccg acctcgacgc
     2881 acaatttcag gatctcggcg ccgaaggcct cggcgcgctc gagctcgtcc ggctggttgg
     2941 cgtcatacag aatcagcggg tgcaggttgc cgtcgccggc gtggaacacg ttggcgacgc
     3001 gcaagccgaa gcgctcggac agctcggcca tgcgcgccag cacctcgggc aggcgcgcgc
     3061 gcgggatggt gccgtccatg cagtagtagt cgggcgagat gcgcccgacc gcgggaaacg
     3121 cgttcttgcg cccggcccag aagctgagcc gctcgtcctc gctccggctg gtgcgcaccg
     3181 tgaccgcgcc gcaggactcg gcgacgccct gcacgcgcgc ggccaggtcg tcgacctcga
     3241 ccgccgggcc gtcgagctcg acgatcagca gcgactcgac gtcgagcgga tagccggcgt
     3301 ggcagaacgc ctcggccgcg tgcaccgcgg cgcggtccat catctccatg ccgcccggga
     3361 tgatgccggc gccgatcacc tcggcgacgc agcgcccgcc ggcgacgacc gagggaaagc
     3421 cgatcagcag cgcgcgcgcg gtctcaggct tcggcagcag gcgcaccgtg acctcggtga
     3481 tcacgccgag cagaccttcc gagccggtca acaggccgag caggtcgtag ccctcggagt
     3541 cgacgtgccg gccgccgagg cgcaccacct cgccgtccat cagcaccagc tcaaggccga
     3601 ggatgttgtt ggtggtgacg ccgtatttga ggcagtgcac gccgcccgag ttctcggcga
     3661 cgttgccgcc gatcgagcac gcgatctggc tcgacgggtc tgg
//
LOCUS       DASWHU010000417         7506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_701551, whole genome shotgun sequence.
ACCESSION   DASWHU010000417 DASWHU010000000
VERSION     DASWHU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701551"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VGB88_15200"
     CDS             complement(<1..558)
                     /locus_tag="VGB88_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEX9648851.1"
                     /translation="MAKPKLDPRDPIFHDDDAAREYLESLLWPGGPVCPRCGVMGDRI
                     TKLKGKSTRPGVHKCKDCRKPFTVTVATVMERSHIPLSKWVLAAQLMASSKKGMSALQ
                     LHRMLGTNYETAWFLFHRLREAADNVPFAPLGGANKVVEADETYIGGKARNKAYGPPP
                     KKMAVFTLVEREGRARSFHVAKVDAK"
     gene            735..1058
                     /locus_tag="VGB88_15205"
     CDS             735..1058
                     /locus_tag="VGB88_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9648852.1"
                     /translation="MDDVLRAIAEPNRRRILHLVWDAELSAGDIAATFEVSRPAISQH
                     LKVLEGAGLVAVRKAGTRRLYRARRQAIADLMAFIEAYWDQGLGRLKDAAETEQRRRR
                     HGGQA"
     gene            1042..1500
                     /locus_tag="VGB88_15210"
     CDS             1042..1500
                     /locus_tag="VGB88_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9648853.1"
                     /translation="MAAKHDQTLEREVYIEATPETVFGYLVEPAKMTRWMGTEATFEP
                     RPGGRYRVNVSGEDVASGQVVAVEANRRVVYSWGWEGGEMLPPGASRVEITLRPSGTG
                     TALTLRHSGLPEALADKHGEGWEHYLARLKTVAAGGEPGRDPWVKDAAAE"
     gene            1539..2204
                     /locus_tag="VGB88_15215"
     CDS             1539..2204
                     /locus_tag="VGB88_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX9648854.1"
                     /translation="MERFSLDSASKAYRARREELRQAEIALKDQVEKVAALRRALPAD
                     TPVKEDYAFTEAGPKGTRTVRLSELFAPGHDALVVYHYMWAPADDAPCPMCTMWTDGY
                     NAVAPHVTRTAPMVVVTKQDAARMLAFAEKRGWRNLRVVSSGSNSFNRDFGMEDAEGH
                     QMPGVSVFLKGKDGRVRHFYTMSAIMGDDNYRGMDLLSPVWNMLDLLPQGRGEWFPSV
                     AYD"
     gene            2294..2644
                     /locus_tag="VGB88_15220"
     CDS             2294..2644
                     /locus_tag="VGB88_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014392996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9648855.1"
                     /translation="MPQFMLLLHNAPRDTSKASPADMQAVVNEYSAWSQKLAAAGKLK
                     GGQKLADEGGRMITAKGGKISVTDGPYTETKEVVGGFFMIEAKSYDDAVKLSSDCPHL
                     KFGGRIELRQIDVM"
     gene            2653..3936
                     /locus_tag="VGB88_15225"
     CDS             2653..3936
                     /locus_tag="VGB88_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9648856.1"
                     /translation="MADARGHGEVGALVDDLFRREAGRLVAMLARIFGPAHLSLAEEV
                     VQDALVRALGRWPYHGIPDRPAAWLATVARHRALDAVRREARLRRLEPEVTRAFERLD
                     DADAAPDAARFARELADDQLGLMFACCHPCLPRPAQVALTLKTLGGFGIAEIARAFLA
                     DEATIAQRITRAKRRIRDHDIAFAIPAPDEIADRRDAVLECLYLMFTEGHAATRGRDL
                     VREEVCRESLRLTLLLAEHPAASAPEADALAALMMFQAARFPARTDARGELKPLEAQD
                     RGLWDARLIAEGQAALGRAMAADELSVYHLEAGIAGCHAAAPSWRETDWPAILGYYDL
                     LLGMRPSPVVALNRAVALAMVEGPERGLAALDAIDHAGALASYHLLPATRGELCFRLG
                     RHAEAWDHFRAALALAATEPERRHLARRIAACAAT"
     gene            complement(3994..4590)
                     /locus_tag="VGB88_15230"
     CDS             complement(3994..4590)
                     /locus_tag="VGB88_15230"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX9648857.1"
                     /translation="MTAGLYPRKRIEAQRVEQNISSSHRIIWLTSMARPLDCGPAPSR
                     FCDGVSGYGVIYAAPQFDTAFIETVVRDRFVHRHRRAVPFGDISERGWVELATRTNEA
                     VVLLDLTGRGCLALGAPTDAVTARNHAAGRALGRAIYREHEDIDGFLYSSRLTGADCY
                     AIFDRAVPRLAALGTGELKDHAALAGVLRDQHIGLIRD"
     gene            complement(4587..5150)
                     /locus_tag="VGB88_15235"
     CDS             complement(4587..5150)
                     /locus_tag="VGB88_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9648858.1"
                     /translation="MSRRENRRDDVVAFQVGGLKGLSKTAAQFVRRRPREARLAVTAA
                     LEAAAVQFESSHESEQRTIPAALRRFGRSAPTADDTIGVAAAAKRLHVSRTTVYDWIK
                     KKRMIAWKTSKRGMAIPAEQIVGPGELVPGLEQVLAIIRDGRAAWRFLNEESPFFDQC
                     RRPIDALRTGDKAAVSAAARAHGEAFT"
     gene            complement(5258..6568)
                     /gene="hisS"
                     /locus_tag="VGB88_15240"
     CDS             complement(5258..6568)
                     /gene="hisS"
                     /locus_tag="VGB88_15240"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9648859.1"
                     /translation="MSQPPLTPPSGSRDFLPREVRRREAVIARIKQVFALHGYEPMET
                     PAFERIEVLAGKYGEESDKLIFKILKRGEKAASGEADLALRYDLTVPAVRLYAHRRNE
                     LPRIFKRYQIGPVWRADRPGRGRFREFHQCDVDIFGSSSALADVDVIVTLSAALAAVG
                     LDRFTIRLNSRHVLHAMMDAYGIAPARHGEALIALDKLDKIGADGVAAELAGRGLGGN
                     VVAALTADLARPDFERVLRARIADSGAGRTGLAEVDAILAHAGGAIGAGEIRFDPLLA
                     RGLDYYTGAIYEFAADGAGGSIAGGGRYDDLAGMFMKESVPICGGSLGIERILMMLDA
                     EGAGAPAPLAYVTVWDEAAIGDALALAASLREAGLAAEIDLTSARLARQFKCADDRGA
                     RFALVRGPDEQAAGTVVVKDLASGAQDSVGLEQVVDHIRRALGG"
     gene            6724..>7506
                     /locus_tag="VGB88_15245"
     CDS             6724..>7506
                     /locus_tag="VGB88_15245"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX9648860.1"
                     /translation="MTRRRSKLSKAYSDPRFIASRDARPVRILAEYLEPLARFEHFHI
                     RDTIVFFGSARALDPVVAAGALERARAERGDVARAERALRLSAYYAAARELARRLTEW
                     SKGLTDKDRRFVICTGGGPGIMEAASRGASEAKGVNIGLTISLPNEEFENPYVTRELA
                     FEFHYFFIRKFWFTYLAKAVVVMPGGFGTLDELFELLTLVQTLKIQKRMPVAMFGAEY
                     WDQVINFDTLVEWGTIDAADSALFHRTDSVDDAFQFITRELTE"
BASE COUNT         1154 a         2615 c         2610 g         1127 t
ORIGIN      
        1 cttagcgtca accttggcga catggaagct gcgcgccctg ccttcacgct cgaccagggt
       61 aaagaccgcc atcttcttcg gcggcgggcc atacgccttg ttgcgcgcct tgcccccgat
      121 gtaggtctcg tcagcctcga ccaccttatt cgcgccgccc aagggggcga acggcacatt
      181 gtcggcggct tcccgcagcc tatggaacag aaaccatgcg gtctcgtagt tggtgccgag
      241 catccggtgg agctgaagcg cggacatacc cttcttgctg gacgccatga gttgagccgc
      301 cagcacccac ttggacagcg ggatatgcga gcgctccatg acggtggcga ccgtcacggt
      361 aaagggcttg cggcagtcct tgcacttatg gacgcctgga cgggtgctct tgcccttcag
      421 cttcgtaatc cggtcgccca tgacgccaca gcgcgggcaa accggaccac cgggccaaag
      481 gaggctttca agatattcgc gcgccgcgtc gtcgtcgtga aagattggat cacgagggtc
      541 caacttgggc ttggccatgg tctcaactcc tattctgagg ccagcctagc acagtgagta
      601 tggtacgtca agcggatagt tcccggaaat tgccgtcgcc gagcggcacc gccggatgct
      661 cgacgaacgg ggcggcggcg aatcgacata aatagtaagt tgacacttac gtaagactat
      721 gcttacgtaa gttcatggac gacgtcctgc gcgccatcgc cgagcccaac cgccggcgca
      781 tcctgcacct ggtgtgggat gcggagctga gcgcgggcga catcgccgct accttcgagg
      841 tctcgcggcc ggccatctcg cagcacctca aggtgctcga aggggcgggc ctggtggccg
      901 tgcgcaaggc cggcacccgg cgcctgtacc gcgcccggcg ccaggcgatc gcggacctga
      961 tggcgttcat cgaagcctat tgggatcagg gtctcggccg gctcaaggac gccgccgaga
     1021 ccgagcagag gagacgacgc catggcggcc aagcatgacc aaaccctcga gcgcgaggtc
     1081 tatatcgagg ccacgcccga gaccgtgttc ggatacctcg tcgagcccgc caagatgacc
     1141 cgctggatgg gcaccgaggc gaccttcgag ccgcgccccg gcggccgcta ccgggtcaac
     1201 gtctcgggcg aggacgtcgc ctcgggccag gtggtcgcgg tcgaggccaa ccggcgcgtg
     1261 gtctacagct ggggctggga gggcggcgag atgctgccgc cgggcgccag ccgggtcgag
     1321 atcacgctcc gcccgagcgg caccggcacc gcgctcacct tgcgccacag cggcctgccg
     1381 gaggcgctcg ccgacaagca cggcgagggc tgggagcact acctggcgcg gctcaagacc
     1441 gtcgccgccg gcggcgagcc cggccgcgac ccgtgggtca aggacgccgc cgccgagtag
     1501 gccggcgcga cgcgcgcaat cgaacaggga gacgagccat ggaacgcttc agtctggact
     1561 cggccagcaa ggcctatcgc gcccgccgcg aggagctgcg ccaggccgag atcgcgctca
     1621 aggaccaggt cgagaaggtc gcggcgctgc gccgcgcctt gccggccgac acgccggtca
     1681 aggaggacta cgcattcacc gaggcggggc ctaagggcac gcgcacggtg cgcctgtccg
     1741 agctgttcgc ccccggccac gacgccctgg tggtctacca ctacatgtgg gcgccggccg
     1801 acgatgcccc gtgtccgatg tgcaccatgt ggaccgacgg ctacaacgcg gtcgcgccgc
     1861 acgtcacccg gaccgcgccg atggtggtgg tgaccaagca ggacgcggcg cgcatgctcg
     1921 ccttcgccga gaagcgcggc tggcgcaacc tgcgcgtggt ctcaagcggc agcaacagct
     1981 tcaaccgcga cttcggcatg gaggacgccg agggccacca gatgcccggg gtgagcgtgt
     2041 tcctcaaggg caaggacgga cgggtgcgcc acttctacac gatgtcggcg atcatgggcg
     2101 acgacaacta ccgcggcatg gacctgttgt cgccggtgtg gaacatgctc gacctgttgc
     2161 cgcaaggccg cggcgagtgg tttccgagcg ttgcctacga ctgacgccct gcagggtgtc
     2221 gattcgccgc cggctggttc gtcgctagca tagacgcccg gcgcttcggc cgggccgagc
     2281 gcaggaggtc gagatgccac agttcatgct gttgttgcac aatgcgcccc gcgacacctc
     2341 gaaggcctcg cctgccgaca tgcaggcggt ggtcaacgag tacagcgcct ggagccagaa
     2401 gctcgccgcc gcgggcaagc tcaagggcgg ccagaagctc gccgacgagg gcgggcgcat
     2461 gatcaccgcc aaaggcggca agatcagcgt caccgacggg ccctacaccg agaccaagga
     2521 ggtggtcggc ggcttcttca tgatcgaggc caagagctac gacgacgccg tcaagctgag
     2581 cagcgactgc ccgcacctca agttcggcgg gcggatcgag ctgcgccaga tcgacgtcat
     2641 gtgacggcgg cgatggccga cgcgcgcggg cacggcgagg tcggggcgct ggtcgacgac
     2701 ctgttccggc gcgaggccgg gcggctggtg gcgatgctcg cgcgcatctt cggccccgcc
     2761 cacctgtcgc tggccgagga ggtggtgcag gacgcgctgg tccgggcgct cgggcgctgg
     2821 ccgtatcacg gcattcccga ccgcccggcg gcgtggctgg cgacggtggc gcgccaccgc
     2881 gcgctcgacg cggtccgccg cgaggcgcgc ctgcgccggc tcgagcccga ggtgacgcgc
     2941 gccttcgagc gcctcgacga cgcggacgcg gcgcccgacg cggcgcggtt cgcgcgcgag
     3001 cttgccgacg accaactcgg cctgatgttc gcctgctgcc acccgtgcct gccgcgcccg
     3061 gcgcaggtgg cgctgacgct caagaccctc ggcggcttcg gcatcgccga gatcgcgcgc
     3121 gccttccttg ccgacgaggc gaccatcgcc cagcggatca cccgcgccaa gcggcgcatc
     3181 cgcgaccacg acatcgcctt cgccatcccc gcgcccgacg agatcgccga ccggcgcgac
     3241 gcggtgctcg aatgcctgta cctgatgttc accgaaggcc acgcggcgac tcggggccgc
     3301 gacctggtgc gcgaggaggt gtgccgggaa agcctgcgcc tgaccttgct gcttgccgag
     3361 cacccggccg ccagcgcgcc ggaggcggat gcgctggcgg cgctgatgat gttccaggcg
     3421 gcgcggttcc cggcgcgcac cgacgcacgc ggcgagctca agccgctcga ggcccaggac
     3481 cgcggcctgt gggacgcgcg cctgatcgcc gagggccagg cggcgctggg gcgcgcgatg
     3541 gcggccgacg agctctcggt ctaccacctc gaggccggca tcgccggctg ccacgccgcg
     3601 gcaccgagct ggcgcgagac cgactggccg gcgatcctcg gctattacga cctgctgctc
     3661 ggcatgcgcc cctcgcccgt ggtcgcgctc aatcgcgcgg tcgcgctggc gatggtcgag
     3721 gggcccgagc gggggctcgc cgcgctcgat gcgatcgacc acgccggcgc gctggcgtcg
     3781 taccacctgc tgccggcgac ccgcggcgag ctgtgcttcc ggctcggccg gcacgccgag
     3841 gcctgggacc atttccgggc cgcgctcgcg ttggccgcga ccgagcccga gcgccgccac
     3901 ctcgcgcgcc ggatcgccgc ctgcgcggcg acgtagcacg ggaggtcagc cgccgagtgc
     3961 gcggcggaga cgctcgatcg cgccgtcggg gtgtcagtcc cggatcaggc cgatatgctg
     4021 gtcacgaagg actcccgcca gtgcggcatg atctttcagt tcgcccgtcc caagggctgc
     4081 gagcctcggc accgcccggt cgaagatggc gtagcagtcg gccccggtca gccgggacga
     4141 gtacagaaac ccatcgatat cttcgtgctc ccgatagatc gcgcgaccca aggcacgccc
     4201 ggccgcatga ttccgtgccg tcaccgcatc ggtcggcgcg ccgagcgcaa ggcatccgcg
     4261 gccggtaaga tccaagagaa ccacagcttc gttcgtcctg gtggcgagtt cgacccagcc
     4321 tcgttccgat atgtccccaa atgggactgc gcggcggtgt cgatgcacga accgatctcg
     4381 cacgacggtc tcgatgaacg cggtgtcgaa ctgcggtgcc gcgtagatga cgccatagcc
     4441 ggacacgccg tcgcagaagc gtgacggggc agggccgcaa tccaaggggc gggccataga
     4501 ggtcagccag atgatgcgat ggctggagga tatgttctgt tcgacgcgtt gggcctctat
     4561 ccgcttgcgc ggatagaggc ccgcggtcac gtgaaggcct cgccgtgcgc tcgcgcggcg
     4621 gcgctcacgg ccgccttgtc accggtcctg agggcatcga tcggacgccg acattggtcg
     4681 aaaaacggcg actcctcatt gaggaagcgc catgccgcgc gcccgtcccg tatgatggcg
     4741 aggacctgtt caagtcccgg caccagctcg ccggggccga cgatctgttc cgccgggatg
     4801 gccatcccgc gcttggacgt tttccatgcg atcatccgct tcttcttgat ccagtcgtag
     4861 accgtggttc gtgatacgtg caggcgtttg gctgctgcgg cgacaccgat cgtgtcatca
     4921 gccgtcggcg cggatcgacc gaaacgcctc agcgctgccg gtatcgttcg ttgttcagac
     4981 tcgtggcttg actcgaactg gaccgctgcg gcttccagcg cggcagtcac ggcaaggcgt
     5041 gcttcgcgcg gacgtcggcg gacgaactgc gccgcggtct tggacaagcc cttcaggcca
     5101 ccgacctgaa aggcgacaac gtcatcgcga cggttttctc gccggctcat cgccgtacct
     5161 cttgtcagtc gaaacccatc gccatgatat cgtccgtgtg tccagtgtgt ccagtttgta
     5221 aagtatggcc gcctgcgcgg cgacgtagca cgggaggtca gccgccgagt gcgcggcgga
     5281 tatggtcaac cacttgctcg agcccgaccg agtcctgggc gcccgaggcg aggtccttga
     5341 ccaccaccgt gccggcggcc tgctcgtcgg ggccgcgcac cagcgcgaag cgcgcgccgc
     5401 ggtcgtcggc gcacttgaac tggcgcgcga gcctggccga ggtcaggtcg atctcggcgg
     5461 caaggcccgc ttcgcgcagg ctggcggcga gcgcgagcgc gtcgccgatt gccgcctcgt
     5521 cccagaccgt gacataggcg agcggcgccg gcgcgcccgc gccctcggcg tcgagcatca
     5581 tcaggatgcg ctcgatgccg agcgagccgc cgcagatcgg caccgactcc ttcatgaaca
     5641 tgccggcgag gtcgtcgtag cggccgccgc cggcgatcga gccgccggcg ccgtcggcgg
     5701 cgaactcgta gatcgcgccg gtgtagtagt cgagcccgcg cgcgagcagc ggatcgaagc
     5761 ggatctcgcc cgcgccgatg gcgccgccgg catgggcgag gatcgcatcg acctcggcga
     5821 gcccggtgcg ccccgcgccc gagtcggcga tgcgcgcacg caagacgcgc tcgaagtcgg
     5881 gccgcgcgag gtcggcggtg agcgccgcca ccacgttgcc gccgaggccg cgcccggcca
     5941 gctccgcggc aaccccgtct gcgccgatct tgtcgagctt gtcgagcgcg atcagcgcct
     6001 cgccgtggcg cgcgggcgcg atgccgtagg cgtccatcat cgcgtgcagc acgtgcctcg
     6061 agttgagccg gatggtgaac ctgtcgagcc cgaccgcggc gagtgccgcc gacaaggtca
     6121 cgatcacgtc gacgtcggcc agtgccgagc tgctgccgaa gatgtcgacg tcgcactggt
     6181 ggaactcgcg gaaccgcccg cgcccggggc ggtcggcgcg ccacaccggg ccgatctggt
     6241 agcgcttgaa gatgcgcggc agctcgttgc ggcggtgggc gtacagcctg accgccggca
     6301 cggtcaggtc gtagcgcagc gcgaggtcgg cctcgccgct ggccgccttc tcgccgcgct
     6361 tgaggatctt gaagatcagc ttgtcgcttt cctcgccgta cttgcccgcc agcacctcga
     6421 tgcgctcgaa cgccggggtc tccatcggct cgtagccgtg cagggcgaac acctgcttga
     6481 tgcgcgcgat caccgcctcg cggcggcgca cctcgcgcgg caggaagtcg cggctgcccg
     6541 agggcggggt tagcggcggt tggctcacgg cgtgcgctcc gtcgtgcccc gggggcgggg
     6601 cgcggcgacc ctacaccgcg ctccggcgcg tgaaaagcgc gccacggcgc cgttgccggg
     6661 gcagcggggc cgccctacct tctggcccgc cccccaatcc cagacagcag aggcaccgcg
     6721 gccatgaccc gccggcgcag caagctttcg aaggcctaca gcgacccccg tttcatcgcc
     6781 agccgcgacg cccggccggt gcgcatcctg gccgagtatc tggagccgtt ggcgcgcttc
     6841 gagcatttcc acatccgcga caccatcgtg ttcttcggct ccgcgcgcgc gctcgatccg
     6901 gtggttgccg cgggcgcgct cgagcgcgcc cgggcggagc gcggcgatgt cgcccgcgcc
     6961 gagcgcgcgc tgcggctgtc ggcctactac gcggcggcgc gcgagctcgc gcgccggctc
     7021 accgaatggt cgaaggggct gaccgacaag gaccgccgct tcgtcatctg caccggcggc
     7081 ggccccggaa tcatggaggc ggcgagccgc ggcgcgtccg aggccaaggg cgtgaacatc
     7141 ggcctgacca tctcactgcc caacgaggag ttcgagaacc cctacgttac ccgcgagctc
     7201 gcgttcgagt tccactactt cttcatccgc aagttctggt tcacttacct ggccaaggcg
     7261 gtggtggtga tgccgggagg cttcggcacc ttggacgagc tgttcgagct gttgaccctg
     7321 gtccagacct tgaagatcca gaagcgcatg ccggtggcca tgttcggcgc cgagtactgg
     7381 gaccaggtga tcaacttcga caccctggtc gagtggggca ccatcgacgc cgccgacagc
     7441 gcgctgttcc atcgcaccga ttcggtcgac gatgcgttcc agttcatcac ccgcgagctg
     7501 accgag
//
LOCUS       DASWHU010000418         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3298598, whole genome shotgun sequence.
ACCESSION   DASWHU010000418 DASWHU010000000
VERSION     DASWHU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298598"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VGB88_15250"
     CDS             <1..1647
                     /locus_tag="VGB88_15250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566139.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX9648861.1"
                     /translation="WDEAVELGTQYGYRNAQATVVAPTGTIGLVMDCDTTGIEPDFAL
                     VKFKKLAGGGYFKIINRIVPQALATLGYAPDEIDRIIAYAVGLGTLDAAPHVNHRTLA
                     AKGFDRAALERIEAALRDAFDIRFVFNKWTLGAEFCRDSLGLDDDRLDDLGFDMLAAL
                     GFSKAEIEAANVHCCGAMTLEGAPHLKAEHLDVFDCATPCGRNGRRALSWRSHIRMMA
                     AAQPFISGAISKTINMPHSATVEDCQDAYRLSWQLGLKANALYRDGSKLSQPLNGLSP
                     ELDADADGAGDAEPARGVPAVERVVEKVVRELVRERWRLPNRRKGYTQKAVVGGHKVY
                     LRTGEYRDGRIGEIFIDMHKEGAAFRSLMNNFAIAISIGLQYGVPLEEFVEAFTFTRF
                     EPSGLVEGNDTIRMATSIIDYVFRELAISYLGRADLAHVEADDLLPDAVGSGERQSQI
                     ADSPQAAADLNALVSPGFVRGRLRVLEGGIAARLEAAAPAGAVAAVGVGIAAGRSVKL
                     TEARIKGYEGDPCPTCGNFTLVRNGTCLKCDTCGDTSGCS"
     gene            1985..2401
                     /locus_tag="VGB88_15255"
     CDS             1985..2401
                     /locus_tag="VGB88_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648862.1"
                     /translation="MRQAKCDASSPRRPLIRAAAVLAVCAQLVGVLPGAVALPVIPRI
                     DDLAAPGAGLQASAVEAAAAPVKTVPARAAKLVAALTGAIARTVGPGDGLMIVDDGRD
                     AVATKIGRCPLRPAQERIAAAGPAFRPSPTGPPHTA"
     gene            2543..3538
                     /gene="secF"
                     /locus_tag="VGB88_15260"
     CDS             2543..3538
                     /gene="secF"
                     /locus_tag="VGB88_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9648863.1"
                     /translation="MRLLRLVPDDTRIDFVRHRTIAFVVTGLLLAVTVAALLTRGLNL
                     GVDFAGGTLIEVRADHALDIGALRVKLAAIGLGDFELQQFGEGNDLSIRVERKPEAES
                     ATVAETVRGALGQGFDFRRVETVGPRVGAELLRDGLLATAFAIVAIAAYVTTRFEWQY
                     GLAAIIAIFHDVATTVGLYAALQIEFNLSSVAALLLLAGYSVNDTIVVFDRIRETVRK
                     RKTGDLRAVINESVNRTLSRTVMTSGTTLLAVLPLVVYGGDALFGFSLALAWGIVVGT
                     YSSIFVAAPLLLYLPSVARAHAPAAAQAPEWTPPSERPGPSPGARPIAGESRPQS"
BASE COUNT          502 a         1346 c         1222 g          526 t
ORIGIN      
        1 tgggacgagg cggtcgagct cggcacccag tacggctatc gcaacgccca ggcgaccgtg
       61 gtcgcgccga ccggcaccat cggcctggtc atggactgcg acaccaccgg catcgagccc
      121 gacttcgccc tggtcaagtt caagaagctc gccggcggcg gctacttcaa gatcatcaac
      181 cggatcgtgc cccaggcgct ggcgaccctc ggctacgcgc ccgacgagat cgaccggatc
      241 atcgcctacg cggtcggcct cggcacgctc gacgccgccc cccacgtcaa ccaccggacg
      301 ctcgcggcga agggcttcga ccgcgccgcg ctcgagcgaa tcgaggcggc gctccgggac
      361 gccttcgaca tccgcttcgt gttcaacaag tggacgctcg gcgccgagtt ctgccgcgat
      421 tcgctcgggc tggacgacga ccggctcgac gacttgggct tcgacatgct cgccgcgctc
      481 ggcttctcga aggccgagat cgaggccgcc aacgtccact gctgcggcgc gatgacgctc
      541 gagggcgcgc cgcatctcaa ggccgagcat ctcgacgtgt tcgactgcgc gacgccctgc
      601 gggcgcaacg ggcggcgcgc gctgtcgtgg cggagccaca tccgcatgat ggcggcggcg
      661 cagccgttca tctcgggcgc gatctccaag accatcaaca tgccccattc ggcgaccgtc
      721 gaggactgcc aggacgccta tcggttgtcg tggcaactgg ggctgaaggc gaatgcgctg
      781 taccgcgacg gctccaagct gagccagccg ctgaacggcc tcagccctga gctcgatgcg
      841 gacgcggacg gggccggcga cgccgagccc gcgcgcggcg tgcctgcggt cgagcgggtg
      901 gtcgagaagg tggtgcgcga gctggtgcgc gagcgctggc gcctgcccaa ccggcgcaag
      961 ggctacaccc agaaggcagt ggtcggcggc cacaaggtgt atctgcgcac cggcgagtac
     1021 cgcgacgggc ggatcggcga gatcttcatc gacatgcaca aggaaggcgc cgccttccgc
     1081 agcctgatga acaacttcgc gatcgcgatc tcgatcgggc tgcaatacgg ggtgccgctc
     1141 gaggagttcg tcgaggcgtt caccttcacc cgcttcgagc cgagcggcct ggtcgagggc
     1201 aacgacacca ttcgcatggc gacctcgatc atcgactacg tgttccgcga actcgccatc
     1261 tcctatctcg gtcgcgccga cctcgcccat gtcgaggccg acgacctctt gcccgacgcg
     1321 gtcggcagtg gcgagcgcca gagccagatc gccgattcgc cgcaggcggc ggccgacctc
     1381 aacgccctgg tcagcccggg cttcgtgcgc gggcggctca gggtgctcga gggcggcatc
     1441 gccgcgcgcc tcgaggcggc ggcgcccgcc ggcgccgtgg ccgcggtcgg cgtcggcatc
     1501 gccgccggcc gctcggtgaa gctcaccgag gcccggatca agggctacga gggcgatcca
     1561 tgcccgacct gcggcaactt tacgctggta cgcaacggga cctgcctgaa atgcgacacc
     1621 tgtggcgaca ccagcgggtg ctcctgagtg gccgggagca ccgatgcgcc gtcttccctc
     1681 tcccggacgg cgcaggtccg cgcatgcgga ccgatctgcc tgtgcgacca catctcgtgg
     1741 tcgattcctc ctgtgacctc ggggcgcggt cgaggcaggc gcgcccctcc ttccctctcc
     1801 cacgcccgcg ccgggcggat gactgagccc cgggcggcgg gcctgtcgcc cgcgaaatat
     1861 atttgcagcc cttagccggg gggccttgta ggcacccctg ggccatgcaa atgtcttggt
     1921 gtggaccgga gcacgcctag gtcgcgccgc ccggtcgcgt gagtacccat ccgccgtgac
     1981 ggccatgcgc caagccaagt gcgacgcgtc ctcgccgcgc cggccgctga tccgggcggc
     2041 ggcggtgctg gccgtatgcg ctcagctcgt cggcgtcctg cccggcgcgg tcgcgctgcc
     2101 cgtcatcccc cgaatcgacg acctcgccgc accgggcgcg gggcttcagg cgtccgcggt
     2161 cgaggccgcg gcggcgccgg tcaagacggt tcccgcccgc gccgccaagc tggtcgcggc
     2221 gctgaccggc gcaatcgcgc gcactgtcgg ccccggcgac ggcctgatga tcgtcgatga
     2281 cggccgcgat gcggtcgcaa ccaagatcgg ccgctgcccg ctccggccgg cgcaggagcg
     2341 catcgccgcc gccggccccg ccttccgccc gagcccgacc ggacctccgc acaccgcctg
     2401 acggcagccc ccgctgccgc gccggacccg cgcccgatag ggcgccgggg ccggctcggg
     2461 gacggccgtc gggccgtcta ttgcgtgcgg acttccgggc cccgccggct caggcgggcg
     2521 cccggctgca agggccatcc acatgcgtct tctgcgcctg gtgccggacg acacccgcat
     2581 cgacttcgtc cgccaccgca cgattgcctt cgtcgtcacc gggctgctgc tggcggtgac
     2641 cgtggccgcg ctgctcacgc gcgggctcaa cctcggcgtc gacttcgccg gcggcacgct
     2701 gatcgaggtg cgcgccgacc atgccctcga catcggagcg ctgcgcgtca agctcgccgc
     2761 gatcggtctc ggcgacttcg agctgcagca gttcggcgag ggcaacgacc tgtcgatccg
     2821 cgtcgagcgc aagcccgagg ccgagagcgc gaccgtcgcc gagaccgtgc gcggcgccct
     2881 cggccagggc ttcgacttcc gccgggtcga gaccgtcggc ccgcgcgtcg gcgcggagct
     2941 gctgcgcgac ggcctgctgg ccaccgcctt cgcgatcgtc gccatcgccg cctacgtcac
     3001 cacccggttc gagtggcagt acgggctggc tgcgatcatc gccatcttcc acgacgtcgc
     3061 caccaccgtc gggctgtacg cggcgctgca gatcgagttc aacctgagct cggtggcggc
     3121 gctgctgctg ctcgccggct actcggtcaa cgataccatc gtcgtcttcg accgcatccg
     3181 cgagacggtg cgcaagcgca agaccggcga cctgcgcgcg gtcatcaacg agagcgtcaa
     3241 ccgcaccctc agccgcacgg tgatgaccag cggcaccacc ctgctcgcgg tgctgccgct
     3301 ggtggtctat ggcggcgatg ccttgttcgg cttcagcctg gcgctcgcct ggggcatcgt
     3361 ggtcggcacc tactcgtcga tcttcgtcgc cgcgccgctg ctcctctatt tgccctcggt
     3421 agctcgcgcc catgcgccgg cagcggcgca agcgcccgaa tggactccac cttccgagcg
     3481 cccggggccc agccccggcg cccgaccgat cgccggcgag agccggccgc agtcgtgacc
     3541 ccggaataac cgaatccggc ggcgccgccg ccggcagata aggaccgcca ccatgt
//
LOCUS       DASWHU010000419         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_330905, whole genome shotgun sequence.
ACCESSION   DASWHU010000419 DASWHU010000000
VERSION     DASWHU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330905"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGB88_15265"
     CDS             complement(<1..237)
                     /locus_tag="VGB88_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX9648864.1"
                     /translation="MTVVTRFAPSPTGYLHIGGARTALFNWLFARHHGGRFLLRIEDT
                     DRARSTEDAVRAIFDGLAWLGLDWDGEAVFQFARM"
     gene            300..>1849
                     /locus_tag="VGB88_15270"
     CDS             300..>1849
                     /locus_tag="VGB88_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9648865.1"
                     /translation="MLGTGDRGAVSGVETSRHGARAAEWLAATLIAERDRWPLWAPVG
                     IGTGIGIYFVLPEEPHLALLAVLLVLALAGLLAVRRAGRAAVMALLAPVLVVAGLALA
                     VIRADLVAAPIIESRHGPAGVTGRVASVEPLESGVRAVIVPSDIERIEAAAMPRRVRL
                     TARGDTAALVPGARVRVPAVLMPPPRPAAPGAYDYARSLYFQRIGAVGYAVGTAVAVA
                     PAGGGGGADVEARLSALRLAVADRIEAAAGGGPNRARAEVAKALLTGIRTGIPDEVVS
                     AWRDSGLAHLLAISGLHLGLVGGFLFFAVRLALASVEAVALRYPIKKWAAAFALVGAF
                     AYLLLTGATVPTQRAFVMAALVLLAVILDREAVSMRPVALAALVVLALHPESMVGASF
                     QMSFAAVIALVATFEAVRGRWTDHRNRRPGVLRRAALYVGFVAMTSVVAGAATAPFAA
                     YHFGRLTTVGLVANLVAVPTTALWIMPWGLAGLLLMPVGLEQLALVPMAWGIELVNAT
                     ARWCAGLPG"
BASE COUNT          186 a          645 c          728 g          290 t
ORIGIN      
        1 catgcgcgcg aactggaaca ccgcctcgcc gtcccagtcg aggccgagcc aggcgaggcc
       61 gtcgaagatc gcccgcaccg cgtcctcggt cgagcgcgcg cggtcggtgt cctcgatcct
      121 gagcaggaac ctgccgccgt ggtggcgggc aaagagccag ttgaacagcg ccgtgcgggc
      181 gccgccgata tgcaaatagc cggtcggcga gggggcgaag cgcgtgacga cggtcatcca
      241 tcctccggca tggcgcgggc gcaggcgtgg tagcataacg cgacttcggg ggcgacggca
      301 tgctcgggac cggcgacagg ggggcggtgt ccggcgtcga gacatcgcgt cacggcgcgc
      361 gcgccgcgga atggctggcg gcgacattga tcgccgagcg cgaccgctgg ccgctgtggg
      421 cgccggtcgg gatcggcacc ggcatcggca tctatttcgt gctgcccgag gagcctcacc
      481 tggcgctgct ggcggtgctg ctggtgctgg cgctggccgg gctgctcgcc gtgcgccgcg
      541 ccggacgggc ggcggtgatg gcgttgctgg ccccggtgct ggtcgtcgcc ggcctcgcgc
      601 tggcggtgat ccgcgccgat ctggtggcgg cgccgatcat cgagtcgcgc cacggtccgg
      661 caggggtcac cggccgcgtg gcctcggtcg agccgctcga gtcgggcgtg cgcgccgtga
      721 tcgtgccgtc cgacatcgaa cggatcgagg cggcggcgat gccgcgccgg gtccggctga
      781 ccgcacgcgg cgacaccgcg gcgctggtcc cgggcgcgcg cgtgcgcgtg ccggcggtgc
      841 tgatgccgcc gccgaggccg gcggcgccgg gcgcatacga ctatgcccgc tcgctctact
      901 tccagcgcat cggcgcggtc ggctatgcgg tcgggaccgc ggtcgcggtt gcgcctgcgg
      961 gcggcggcgg cggcgcggac gtcgaggcgc ggctgtcggc gctccgcctg gcggtcgccg
     1021 atcgcatcga ggccgcggct ggcggcgggc ccaaccgggc ccgggccgag gtcgcgaagg
     1081 cgctgctgac cgggatccgc accggcatcc cggacgaggt ggtctcggcc tggcgcgatt
     1141 ccggcctcgc ccacttgctc gcgatctccg gcctgcacct gggattggtc ggcggattcc
     1201 tgtttttcgc cgtgcgcctg gcgctggcgt cggtggaggc ggtggcgctc cgctacccga
     1261 tcaagaagtg ggccgcggcg ttcgccctgg tcggcgcctt cgcctatctg ctgctgaccg
     1321 gcgccacggt gcccactcag cgcgccttcg tcatggcggc gctggtgctg ctcgccgtga
     1381 tcctcgaccg cgaggcggtg tcgatgcgcc cggtcgcgct ggcggcgctg gtggtgctgg
     1441 cactgcatcc ggagagcatg gtcggcgcga gcttccagat gtcgtttgcg gcggtgatcg
     1501 cgctcgtcgc caccttcgag gcggtgcgcg gccggtggac cgaccatcgt aaccggcgtc
     1561 ccggcgtgct ccggcgcgcg gcgctctatg ttggcttcgt ggccatgacc tccgtggtcg
     1621 ccggcgccgc gaccgcgccg ttcgccgcct accatttcgg ccgcctgacc accgtcggcc
     1681 tggtcgccaa tctggtcgcg gtgccgacca cggcgctgtg gatcatgccg tgggggctcg
     1741 ccggcctgct gctgatgccg gtcgggctcg agcagctggc gctggtgccg atggcctggg
     1801 ggatcgagct ggtcaacgcc accgcgcgct ggtgcgccgg cctgccggg
//
LOCUS       DASWHU010000420         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_709814, whole genome shotgun sequence.
ACCESSION   DASWHU010000420 DASWHU010000000
VERSION     DASWHU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709814"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB88_15275"
     CDS             <1..277
                     /locus_tag="VGB88_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX9648866.1"
                     /translation="RLAESEELGQRRAMLEVAMGRLNDRERHIFTERRLREDPKTLEE
                     LSQVYGISRERVRQIEVRAFEKVQKAVRDSLAEQTKATVGEREVEPA"
     gene            complement(411..1703)
                     /locus_tag="VGB88_15280"
     CDS             complement(411..1703)
                     /locus_tag="VGB88_15280"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX9648867.1"
                     /translation="MANVAVVGAQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLV
                     IDGATFKLSLLPSGVVRPGKLSMIGSGVVVDPWALIEEIDRLAGQGVTVTPESLRVSE
                     SACLILPLHGELDRHRESARGARMIGTTGRGIGPAYEDKVARRAVRMCDLADAGLLAE
                     RIAAALDHHNPLRRALGLAAVDAGELCARLADVAPRILPFVGPVGRELYQARRLGKRI
                     LFEGAQGAMLDVDHGTYPFVTSSNTLAGSAAVGAGIGPRAIDFVLGITKAYTTRVGRG
                     PFPTEQDNEVGRTLGERGKEFGTVTGRARRCGWFDAVLVRQAVQLGGIDGLALTKLDV
                     LDGFETLKVCTGYRIDGRPFDYLPAAPTLQAAAEPQYETMDGWRESTRGARSWIDLPA
                     TAIKYIRRIEELIGAPVALLSTSPERDDTILVRDPFAD"
     gene            complement(1719..2426)
                     /locus_tag="VGB88_15285"
     CDS             complement(1719..2426)
                     /locus_tag="VGB88_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX9648868.1"
                     /translation="MSPDRAQIAAGGPAGGAVVVLPSPPCGPKPPAFAKPARAGEGRS
                     GDGPKRSRLGFAQAGEAARLLAKLVDAAGPAEAGMPALARLELPQAAAELVQRLAEVV
                     ALVRAAAPGLSLTVDAVESRGFEYHTGVSFTLFALGVRGELGSGGRYRTASGEPATGF
                     TLFTDTLLRAAPAPEPRRRLLLPHGAPAEVARRLRADGWVVVQGLVATGDDAAEARRL
                     GCGHLWRAGDIAAVARG"
     gene            2841..3905
                     /locus_tag="VGB88_15290"
     CDS             2841..3905
                     /locus_tag="VGB88_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9648869.1"
                     /translation="MKTIGIAVIAGALGVAVLLPGGAALAVNDEPLTDKWAPSEWGPD
                     DKAGAVNRTTPAMVMKAAGLVKRGKVATLGKVYQQDAPAFGSRGWRMTIPGLPTGGPF
                     GDIGLMYNDEYLSTEIGQIGTQFDGPGHIGVKTSKGDFFYNGRYLTDPDVGTYGMGPL
                     GVEHVAKIGFVCRGVLLDAVALRGGQLPIPKAEKGGSDPGVITANDIDDMTRNQGIDP
                     IGEGDCVFLYTGHGNIWDPNKWDTFDAAEKTKRIAEFNSGEPGYGDSACELLASRKII
                     LWGSDTWASEAVAPDFTGETEEVLPCHVRMMPKRGIWNLENMDLSQLVADQAYEFLFA
                     WSPLKIKGGTGSPGNPIAMY"
BASE COUNT          613 a         1416 c         1452 g          565 t
ORIGIN      
        1 gcgcctcgcc gagagcgagg agctcggcca gcgccgggcc atgctcgagg tggcgatggg
       61 ccggctgaac gaccgcgagc gccacatctt caccgagcgg cgcctgcgcg aggacccgaa
      121 gacgctcgag gagctgagcc aggtctacgg catcagccgc gagcgggtgc gccagatcga
      181 ggtgcgcgcg ttcgagaagg tccagaaggc ggtgcgcgac agcctggcgg agcagaccaa
      241 ggccaccgtc ggcgagcgcg aggtcgagcc cgcctgagtt cggcggcgcc gtcgcgcctg
      301 ctacgcgact caccctcacc cggcggcttc gccgccaccc tctcccgcaa gcgggagagg
      361 gaggggcccg ccgcgaagcg gcgggagggt gagggcaatg gcgccggcgc tcagtcggcg
      421 aacgggtcgc ggaccaggat ggtgtcgtcg cgctcggggc tggtcgagag cagcgctacc
      481 ggcgcgccga tcagctcctc gatccggcgg atgtacttga tcgcggtcgc cggcaggtcg
      541 atccacgagc gcgcgccgcg cgtgctctcc cgccagccgt ccatggtctc gtactggggc
      601 tccgccgccg cctgcagcgt gggcgcagcc ggcaggtagt cgaacggccg gccgtcgatc
      661 cggtagccgg tgcacacttt caaggtctcg aagccgtcga gcacgtcgag cttggtgagc
      721 gccagcccgt cgatcccgcc gagctgcact gcctggcgca ccagcaccgc gtcgaaccag
      781 ccgcagcgcc gcgcccgccc ggtgacagtg ccgaactcct tgccgcgctc gccgagggtg
      841 cggccgacct cgttgtcctg ctcggtcggg aacgggccgc ggccgacccg ggtggtgtag
      901 gccttggtga tgccgagcac gaagtcgatc gcgcgcgggc cgatgccggc gccgaccgcg
      961 gcgctgccgg caagcgtgtt cgacgaggtg acgaacgggt aggtgccgtg gtcgacgtcg
     1021 agcatcgcgc cttgcgcgcc ctcgaacagg atgcgcttgc cgaggcggcg ggcctggtac
     1081 agctcgcgcc cgaccgggcc gacgaacggc aggatgcgcg gcgccacatc ggcgaggcgc
     1141 gcgcacaact cgccggcatc gaccgcggcg aggccgagag cgcggcgcag cgggttgtgg
     1201 tggtcgagcg cggcggcgat ccgctcggcc aagaggccgg cgtcggcgag gtcgcacatg
     1261 cgcaccgcgc gccgcgccac cttgtcctcg taggccgggc cgatgccgcg cccggtggtg
     1321 ccgatcatgc gcgccccgcg cgcgctctcg cgatggcggt cgagctcgcc gtgcagcggc
     1381 aggatcaggc acgcgctctc cgagacgcgc aaggactccg gggtgacggt gacgccctgg
     1441 ccggcgaggc ggtcgatctc ctcgatcagc gcccacgggt cgaccaccac gccgctgccg
     1501 atcatcgaca gcttgcccgg gcgcactacg cccgagggca acaggctcag cttgaaggtg
     1561 gcgccgtcga tgaccagggt gtggccggcg ttgtggccgc cctggaagcg gaccacgatg
     1621 tcggcgcgct cgctcagcca gtcgacgatc ttgcccttgc cctcgtcgcc ccactgggcg
     1681 ccgaccaccg cgacgttggc catgcccggc cccgctgcct agccgcgcgc gacggcggcg
     1741 atgtcgccgg cgcgccacag atggccgcag ccgaggcgcc gcgcctcggc cgcatcgtcg
     1801 ccggtcgcga ccagcccttg caccaccacc cagccgtcgg cgcgcaggcg ccgcgcaacc
     1861 tcggccggcg cgccgtgggg cagcaacagg cgccggcgcg gctcgggcgc gggcgcggcg
     1921 cgcagcagcg tgtcggtgaa cagggtgaag ccggtcgcgg gctcgccgct cgcggtccgg
     1981 taacgcccgc ccgagccgag ctcgccgcgc acgccgagcg cgaacaaggt gaacgagacg
     2041 ccggtgtggt actcgaagcc gcggctctcg accgcatcga cggtgagcga cagccccggc
     2101 gccgcggcgc gcaccagggc caccacctcg gcgagccgct ggaccagctc ggccgccgcc
     2161 tgcggcagct ccaggcgcgc gagcgccggc atcccggcct cggccggccc cgccgcgtcc
     2221 acgagcttgg ccagcagccg cgccgcctcg cctgcctgcg cgaagccgag gcggcttcgc
     2281 ttcggcccgt cgcccgacct tccttcgccc gcccgagcgg gcttcgcgaa ggcgggaggc
     2341 tttgggccgc agggcggcga aggcaggacg acgactgccc cgccggcggg gccgccggcg
     2401 gcgatctgtg cccgatccgg ggacacggca tcctcccgtt gatgttcctg ttttgttctt
     2461 gccccaaccg cgccgcggag tcaagggcgg cgcgaacacg acggctctcc gggatgccgc
     2521 ccgggatgcc gcccgggatg ccgcccggga taccgccaag acgacacctg accgatcgga
     2581 tgtcggctcc agagcgggat ttgggcctcg acgcccgcgc cgccggcacc gtccgatgtt
     2641 tgacgaacgg cccgcatggt gcaaacatcg ttgccggtgg acagccgtcc gggggatgtc
     2701 cggtgagtcg actgtccggt tatccgcgac gagctcccct cttcgcttga tcgtcagcaa
     2761 acggggagag ccatttgcag aacccgggtc gcgacgggct tgctgttgcc cgtctggcct
     2821 aacgttgttg ggaggacaaa atgaaaacga ttggaatcgc ggtcattgct ggtgcactcg
     2881 gcgtcgccgt gctgctgccc ggcggcgccg cgttggccgt caatgacgag ccgctgaccg
     2941 ataagtgggc gccgagcgaa tggggcccgg acgacaaggc cggcgccgtc aaccgcacca
     3001 cgccggcgat ggtcatgaaa gcagccgggc tggtcaagcg gggcaaggtc gcgaccctcg
     3061 gcaaggttta ccagcaggac gccccggcct tcggctcgcg cggctggcgc atgacgatcc
     3121 cgggcctgcc gacgggcggc ccgttcgggg acatcgggct aatgtacaac gacgagtatc
     3181 tgtccaccga gatcggccag atcggtactc agttcgatgg tcccgggcac atcggggtaa
     3241 agacgtcgaa gggcgatttc ttctacaatg gccgttacct gaccgatccg gatgtcggca
     3301 cttacggaat gggcccgctc ggcgtcgagc acgtcgccaa gatcggcttc gtttgccgcg
     3361 gcgtgctgct cgacgcggtc gctttgcgcg gcggtcagtt gccgatcccg aaggcggaga
     3421 agggcgggtc tgatccgggc gtcatcacgg cgaacgacat cgatgacatg accaggaatc
     3481 agggcatcga cccgatcggc gagggcgact gcgtgttcct gtataccggg cacggcaaca
     3541 tctgggatcc caacaagtgg gataccttcg acgccgccga aaagacgaag cgcatcgccg
     3601 agttcaactc cggcgagccc ggctatggcg actccgcttg cgaattgttg gcgtcgcgga
     3661 agatcatcct gtggggatcg gatacctggg cttcggaagc ggtcgcgccg gacttcaccg
     3721 gcgagaccga ggaagtgttg ccctgccacg tccgcatgat gcccaagcgc ggtatctgga
     3781 acctggagaa catggacctg tcgcaactgg tcgccgacca ggcctacgag ttcctgttcg
     3841 cctggtcgcc gctgaaaatc aagggcggca cgggctctcc gggaaacccg atcgcgatgt
     3901 actaggatcg tagcctcaag accatcgacc ttgtggtcgc gaaggcgggg gcgtgccatc
     3961 ggcacgcccc ttgtctttgc cgatcacgcg atggccaaac acacgttgcg gcgcgcggcc
     4021 atccgccagc ccaacttcca gtggcc
//
LOCUS       DASWHU010000421         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2567428, whole genome shotgun sequence.
ACCESSION   DASWHU010000421 DASWHU010000000
VERSION     DASWHU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567428"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VGB88_15295"
     CDS             complement(<1..1205)
                     /locus_tag="VGB88_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9648870.1"
                     /translation="MPSPPVTVAEIVARFVAARAADRVFGLQGGHIQPIWDQLVRLGV
                     PVIDARDERAAVHMAHAYGELTGRLGVALVTAGPGVTNAVTGVANAHAARAPLLVIGG
                     APPRPQDGLGALQALPHVALMRPITRFAETARVPEQVPRLLDRACAAAAGEGGAPGPA
                     FVEVPTDVLRAAVPDAMVLAEHFAARAPRRRAPDAGLAADAAQLVWAARRPLVISGRG
                     ARGAGRALVRLLDASGAVHLDTQDCRGVVADDHPASVGAVRARAMAEADLVLLAGRRL
                     DYQLGYGSPAVLPNATLVRVSDLVEELADNRRGAVELHADVAAGLDAITDAAGNAVPA
                     ADRTWTKALRAEHEARRASLAETMASAPAGEDGFMHPYRLIGAVNAALDAEAVVAVDG
                     GDILSFARIA"
     gene            complement(1449..>1874)
                     /locus_tag="VGB88_15300"
     CDS             complement(1449..>1874)
                     /locus_tag="VGB88_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648871.1"
                     /translation="FLRDLRKQGGRMPDLIVVATDANCQGLNDRVREFDGPGAPAPMI
                     LAIPDPHVERWLLLDGAAFRAVFGTGCDAPDLKCSKDRYKQRLIEAISATGVTPTLGG
                     IEFAEDIVREMDLGRASRADRSFKRFVDEFRNILRGWQP"
BASE COUNT          279 a          688 c          652 g          255 t
ORIGIN      
        1 gcgatgcgcg cgaagctcag gatgtcgccg ccgtcgaccg ccaccaccgc ctccgcatcg
       61 agcgcggcgt tgaccgcgcc gatcagccgg tagggatgca taaagccgtc ctcgccggcg
      121 ggcgcggagg ccatggtctc ggcgaggctg gcgcggcgcg cctcgtgctc ggcgcgcaac
      181 gccttggtcc aggtgcggtc ggccgcgggc accgcgttgc cggcggcgtc ggtgatggcg
      241 tcgagcccgg cggccacgtc cgcgtgcagc tcgaccgcgc cgcggcggtt gtcggcgagc
      301 tcctcgacca agtcggagac gcgcaccagg gtcgcgttcg gcaacaccgc cggcgagccg
      361 tagccgagct ggtagtcgag gcggcggccg gcgagcagca ccaggtcggc ctcggccatc
      421 gcgcgtgcgc gcaccgcgcc gaccgaggcg ggatgatcgt ccgccaccac gccgcggcag
      481 tcctgggtgt cgagatgtac cgccccgctc gcgtcgagca ggcgcaccag cgccctgccc
      541 gcgccacgcg cgccgcgccc ggagatgacc agcggccggc gcgccgccca caccagctgc
      601 gccgcgtccg ccgccagccc ggcgtcgggc gcgcgccgcc gcggcgcgcg ggcggcgaaa
      661 tgctccgcga gcaccatcgc atctggcacc gcggcgcgca gcacgtcggt cggcacctcg
      721 acgaacgccg ggccgggcgc gccgccctcg cccgccgccg cggcgcacgc ccggtcgagc
      781 agccgcggta cctgctcagg gacccgcgcg gtctcggcga agcgggtgat cgggcgcatc
      841 agcgcgacat ggggcagcgc ttgcaacgcc ccgagcccgt cctgcgggcg cggcggcgcg
      901 ccgccgatca ccaagagagg cgcgcgcgcc gcgtgcgcgt tggcgacccc ggtgacggcg
      961 ttggtgacgc cgggccccgc ggtcaccagt gcaaccccga ggcggccggt gagctcgcca
     1021 taggcgtgcg ccatgtgcac cgcggcgcgc tcgtcgcgcg cgtcgatgac cggcacgccg
     1081 aggcggacga gctggtccca gatcggctgg atgtggccgc cctggaggcc gaacacccgg
     1141 tcggcggcgc gcgcggcgac aaagcgggcg acgatctcgg cgacagtaac gggcggcgac
     1201 ggcatcggcg cttgcggggc catatcgctt gcggggctat gccacgccca cgcgcgcggt
     1261 ctggactcgt ccggcacttc gtccggtagg ccaaagccgg tcctgagccg cgctgctggc
     1321 tgccagtcca cgccgtaccc gccagacgtg agaaatcgag atatgcctgt cccgcacttc
     1381 tttcgtatgc actcggtcca gcgccgcctg ctcgaccaga aacgcaccct caataagccg
     1441 tttgtgggtc aaggctgcca acctcggaga atgttccgga actcatcaac gaaacgcttg
     1501 aacgacctgt ccgccctgga cgctcggccc aagtccattt ctcggacgat atcttcggcg
     1561 aactcgatac ccccgagcgt gggcgtaaca cccgttgcgg atattgcttc gatcagacgt
     1621 tgcttatagc gatccttgct gcatttcaga tcgggcgcgt cgcaacctgt gccgaacaca
     1681 gctctaaacg cagcgccgtc gagcaacagc cagcgctcaa catgcggatc gggaatggca
     1741 aggatcatcg gcgccggtgc acccggcccg tcgaactccc tgacacggtc attcaagcct
     1801 tggcagtttg cgtctgtggc gacgacgatc aggtcaggca tccgaccgcc ttgcttcctg
     1861 aggtctcgca agaa
//
LOCUS       DASWHU010000422        10317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3642266, whole genome shotgun sequence.
ACCESSION   DASWHU010000422 DASWHU010000000
VERSION     DASWHU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3642266"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VGB88_15305"
     CDS             <1..416
                     /locus_tag="VGB88_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648872.1"
                     /translation="LAASQPFRMELARAGERYLGRDGLTDSSRTICRFFLDHAFDGRI
                     MIALAFGLPPILIAALIIVKMRPSKNPRPTEQPDPELTRITTLGLFSAAGAAPFATLF
                     LLLEMIGVGLIAKITRTAKALRLNLLLERALRAVN"
     gene            complement(409..1071)
                     /locus_tag="VGB88_15310"
     CDS             complement(409..1071)
                     /locus_tag="VGB88_15310"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEX9648873.1"
                     /translation="MRSWECWKQGVSAVQIQRMTGLSYKAAWFACHRIREAMKKESLA
                     GKLGIGGGIVEADETYIGGKARDNRHRNRKRSKKVTVMTLIDREGGARTFKIPNTKKG
                     TLQGVIGPNVDRSAVIMTDENPSYEGLQDRFKAHHAVNHSETFVRAIIIHTNFAESYH
                     ALLKRSIIGTHHHLSEKHLHRYLREREFHWNRRHATDGERTIDAIQGAKGKRLMYRTP
                     TN"
     gene            complement(1080..1811)
                     /locus_tag="VGB88_15315"
     CDS             complement(1080..1811)
                     /locus_tag="VGB88_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648874.1"
                     /translation="MPGRGGELAGRIALITGASRGIGAAVAERFAAEGAEVYALARSE
                     DGLAALGERIRAAGAAATLVPLDLTDGTAIDRLGGTLAERHRRLDILIANAAVLGTLG
                     PLGHLRPHVWDRVIEVNLTANWRLIRSLDALLRQSDAGRAVFVTSGVARRATAYWGAY
                     AVSKAAVEMLARLYAAEVANTKVRVNLLDPGAVRTAMRAQAMPGEDPNTLPPPEAITD
                     SFVELASPACRRHGEVVRAAVVRAT"
     gene            complement(1817..3298)
                     /gene="purF"
                     /locus_tag="VGB88_15320"
     CDS             complement(1817..3298)
                     /gene="purF"
                     /locus_tag="VGB88_15320"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9648875.1"
                     /translation="MAARSTMHPWDDDKLREECGIFGLYGHPDAAKHTALGLHALQHR
                     GQEAAGIVSYDGRVFHTHRSLGHVGDIFSSPAVMAALAGERAIGHVRYSTQGETMLRN
                     VQPIFADLEGGGLAIAHNGNLTNALQLRRELVKRGCIFQSTMDTEVIVHLIATSAATG
                     FLDRLVDALGHVQGAYSLILLTGEAMIGVRDRFGVRPLVLGRIPGPDPGPGGYILTSE
                     TCALDIIGARHVRDVEAGEVVVIDADGVRTLKPFARAPSKFCVFEYIYFSRPDSVVEG
                     RNVYQARKRIGAELARESGVPADVVVPVPDSGVPAAMGYAEQAGLPFELGIIRNHYVG
                     RTFIEPSDSIRHLGVKLKHNANGAVLAGKRVVLVDDSIVRGTTSTKIVEMVREAGARE
                     VHMRIASPPTTNSCFYGVDTPVREQLLASHMDVAEMARHIGVDSLAFVSPDGLYRAMG
                     EAGRDAERPQFCDACFTGDYPLPLVDHDGGEGPVQLSLLAESV"
     gene            complement(3312..4043)
                     /locus_tag="VGB88_15325"
     CDS             complement(3312..4043)
                     /locus_tag="VGB88_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9648876.1"
                     /translation="MEQQTVNLVDLGVALVVLVSAVFAFYRGFVRETLAIAGWVGAAV
                     VTYYFFTDARSYVAEYIADEFLVNVATGAGLFLAALVALWLVIHLIVIRVKDSPLNAL
                     DRSLGFLFGVARGVVVVALAYMVGEMTLWDDADHPRPEWLEDARSRPLIDYAAGLIVA
                     VTPKDFLNLPIEKLSEMRNARGLTGGNGSPGTPSEVEQLSRPPVAAGAPTASDPGYRD
                     SESQEMDRLIDSLKDKPAAEGQGSQ"
     gene            complement(4080..5477)
                     /gene="radA"
                     /locus_tag="VGB88_15330"
     CDS             complement(4080..5477)
                     /gene="radA"
                     /locus_tag="VGB88_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9648877.1"
                     /translation="MSAPRTRAAASRYVCQACGASQPRWAGRCEACGAWNTVVEEAAP
                     EAVPKGLGKAKGKRLRLSALTDSAPQTARLATGIAELDRVCGGGLVAGTTVLVGGDPG
                     VGKSTLMLQALAAPARAGARALFVSGEESEEQVRLRAHRLGIADAPVSLAAATSVRDI
                     LATLDDADATQVLVIDSIQTMYVDTLDSAPGTVAQVRASAQELIRLAKRRGVVLVLVG
                     HVTKEGQIAGPRVLEHMVDTVLYFEGERGHQFRILRAVKNRFGATDEIGVFEMTDGGL
                     REVANPSRLFLSERAQAVSGAAVFAGVEGTRPMLVEIEALVSPSPFGTPRRAVIGWDS
                     ARLAMVLAVLDARCGLAVGARDVYLNVAGGLRIAEPAADLAVAAALVSSLTDTPMPGR
                     TIVFGEIGLSGEVRPVAHSEARLKEAAKLGFEQAWLPPARSPVKAALELVEIARLADL
                     IAAFGEPAVRTALAR"
     gene            complement(5474..6250)
                     /locus_tag="VGB88_15335"
     CDS             complement(5474..6250)
                     /locus_tag="VGB88_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9648878.1"
                     /translation="MNAPAKILLDGVCKRFGDNIVLDGIDLEVRTGESLVVIGGSGSG
                     KSVMLKCILGLITPEAGRILIDGEDVVRLAGAEREQINQKFGMLFQGSALFDSLSVWQ
                     NVAFGLIQGRGLARAEAKRIASETLAAVGLGREVGELYPAELSGGMRKRVGLARAIAA
                     RPEIIFFDEPTTGLDPIMGDVIDDLIVQCVRELGATALSITHDMASARKISDRIAMLY
                     KGKIIWCGPTANIDSSDNAFVDQFVHGRAEGPIKMEVRAL"
     gene            complement(6247..7020)
                     /locus_tag="VGB88_15340"
     CDS             complement(6247..7020)
                     /locus_tag="VGB88_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9648879.1"
                     /translation="MNGLALIGRVFLAFMETTGRVTLFAATGVSHCVRPPLYPRLIAR
                     QMLDIGYYSLPVVGLTGLFAGMVLALQSYTGFSRFSAETAIPSVVVISITRELGPVLT
                     GLMVAGRIGASIAAEIGTMRVTEQIDALTTLSTNPFKYLVAPRIVAGVTMLPLLVLVA
                     DCIGVLGGYLVGVNKLGFNGANYIDSTWENLETLDVVSGLVKAAAFGLIIALMGCYHG
                     YHSRGGAQGVGTATTNAVVSASILILTANYFITEVFFAK"
     gene            complement(7017..8153)
                     /gene="alr"
                     /locus_tag="VGB88_15345"
     CDS             complement(7017..8153)
                     /gene="alr"
                     /locus_tag="VGB88_15345"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9648880.1"
                     /translation="MSAADRAGARLIIDLAAIARNWRALKARIGAGVECAAVVKADAY
                     GLGMARVAPALAAAGCRSFFVATLDEGLGLKRLVPEAAIYVLNGAMPGAEGEFAAAAL
                     VPVLNDLGAIARWAGEAGRRTQSLPAAIHLDTGMSRLGLSEREARRLAGAPDLLSGLA
                     PQLVLSHLACAENPGHQMNAAQRVRFAATIAGLPAMRASLANSSAILLGAAYHFDMVR
                     PGAALYGINPSPAGPNPMAQVVRMQGKIIALRDVDRAMTVGYGATHRLARKGRIATIP
                     VGYADGFPRALGNLAQAALAEPAQGAVLVPVVGRVSMDLITLDVSDVPADSARLGAWV
                     DLIGGARPLDDVAAEAGTIAYEILTRLGARLPRLYVEDAAEGAR"
     gene            complement(8193..9719)
                     /locus_tag="VGB88_15350"
     CDS             complement(8193..9719)
                     /locus_tag="VGB88_15350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9648881.1"
                     /translation="MDPLPFVPDPQPERDDTATLGYREMPHNDQAEQALLGAVLVDNA
                     AFDPVSTFLEPEHFFVPVHGRIFAAARALISRGQIANPVTLKAYFETDESLADIGGAQ
                     YLARLAGAAVSLINAEDYGRLVHDLSLRRGLIHIGGEIVDTAFDPRIEDSAAAQIEGA
                     EAKLYELAETGREEGGFKPLSSALTEAILSAEAAYKREGRLSGVATGLIDLDNLLGGL
                     HDSDLVILAGRPSMGKTALATTIAFHAARTYRTEADAAGRPVPVDGAVVGFFSLEMSA
                     EQLAMRILAEAAKIRSDHIRRGKLSNDDFMRLVDASRALSAAPLFIDDTPALSVPALR
                     TRARRLKRTHGLSLVVVDYLQLMRPAGRHDSRVQEISEITQGLKALAKDLDLPVLALS
                     QLSRAVELREDKRPQLADLRESGTIEQDADVVMFVYREEYYLERAEPQQRQDESGDRF
                     ADRYRRWQERFESAAGLADVLIAKQRHGPIGNVKLTFTAETTKFDSYAPADHLPEATH
                     "
     gene            9895..>10317
                     /locus_tag="VGB88_15355"
     CDS             9895..>10317
                     /locus_tag="VGB88_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9648882.1"
                     /translation="MKLYMHPVSTTSRPILLFIAENKIDVELQVVDLMTGEHFQEPYV
                     RINPNRLVPVLEDGDFRLTESSAILKYLADKIDSPAYPKDLRKRARVNEVMDWFNSNF
                     GRDYCYGLVYPQIFPNHKRPDATVQAAVLARGKEGAQTW"
BASE COUNT         1573 a         3537 c         3614 g         1593 t
ORIGIN      
        1 cgttggcggc cagccaaccg tttagaatgg aacttgccag agctggcgaa aggtacttag
       61 gccgcgacgg gttaacagat tcaagccgta ccatatgccg ctttttcctc gatcatgcgt
      121 ttgatggccg catcatgatt gcgctcgcgt ttggacttcc tccaattctt attgcagcac
      181 ttattatcgt gaagatgagg ccgtcaaaga acccgaggcc caccgaacaa cctgaccccg
      241 aactaactcg tattacgaca ctgggcttat tctcggcagc tggtgccgcg ccgttcgcga
      301 cgctttttct gttgctagag atgataggcg ttgggctaat tgcgaagatt acgcgaacgg
      361 ctaaagcgct tcggctgaat ttattgctag agcgtgcgtt acgtgccgtt aattagttgg
      421 agtccgatac atcaaccgct tccccttcgc gccctgaatt gcatcaatcg tccgctcccc
      481 gtcagtcgca tgacggcgat tccagtgaaa ctcccgctcc cgcagataac gatgcaagtg
      541 cttctccgac agatggtgat gcgtcccgat gatcgagcgc tttaggagcg cgtgatacga
      601 ctccgcaaaa ttggtgtgga taatgatggc gcgaacgaac gtctcggaat ggttgacggc
      661 atgatgtgcc ttgaagcgat cttgtagccc ttcataggac gggttctcgt cggtcataat
      721 gacggctgac ctatcgacgt ttgggccgat cacaccttgt aacgttccct tcttcgtgtt
      781 cgggattttg aaagtgcgcg ctccgccctc gcggtcgatc aacgtcatga ccgtgacttt
      841 cttcgagcgc ttgcggttgc gatgacggtt gtcgcgagcc ttcccgccga tgtacgtttc
      901 gtccgcttcg acgataccac cgccaatgcc gagcttgccc gccagtgact ctttcttcat
      961 agcctcgcgg atgcgatgac aagcgaacca agccgcttta tagcttaacc cggtcatgcg
     1021 ctgtatctgg acggcgctaa cgccttgttt ccagcattcc caggacctca ccgcgcgctc
     1081 tacgttgcgc ggacgaccgc cgcccgcact acctcgccgt ggcggcggca ggcggggctg
     1141 gcgagttcga cgaagctgtc ggtaatcgct tcgggcgggg gcaaggtatt cgggtcctcg
     1201 ccgggcatcg cctgggcgcg catggcggtg cggacggcgc cggggtcgag caggttgacc
     1261 cggaccttgg tgttggcgac ctcggcggcg tagaggcggg cgagcatctc gaccgccgcc
     1321 ttgctcaccg cataggcgcc ccaatatgcc gtcgcgcggc gcgcgacgcc tgaggtgacg
     1381 aacaccgcgc gccctgcgtc cgactgcctg agcagcgcat cgaggctccg gatcaggcgc
     1441 cagttggcgg tgagattgac ctcgatcacc cggtcccaga catgcgggcg caggtgcccg
     1501 agcggcccca gcgttcccag caccgcggcg ttggcgatca ggatgtcgag ccggcggtgg
     1561 cgctcggcga gggtgccgcc gaggcggtcg atggcggtgc cgtcggtgag gtcgagcggc
     1621 accagggtgg cggcggcgcc ggcggcgcgg atgcgctcgc cgagcgccgc gaggccgtcc
     1681 tcggagcggg ccagcgcata gacctcggcg ccctcggcag cgaagcgctc ggcgaccgcc
     1741 gcgccgatgc cgcgcgaggc gccggtaatc agcgcgatgc gcccggcgag ctcgccgccc
     1801 cgcccgggca cggcgctcag acgctctcgg cgagcaggga cagctggacc gggccctcgc
     1861 cgccgtcgtg gtcgaccagc ggcagcggat agtcgccggt gaagcaggcg tcgcagaact
     1921 gcggccgctc ggcgtcgcgc ccggcctcgc ccatcgcgcg atacagcccg tcgggggaca
     1981 cgaaggcgag cgagtcgacg ccgatgtggc gcgccatctc ggccacgtcc atgtgcgagg
     2041 cgagcagctg ctcgcgcacc ggcgtatcga cgccgtagaa gcacgagttg gtggtcggcg
     2101 ggctggcgat gcgcatgtgc acctcgcgcg cgcccgcctc gcgcaccatc tccacgatct
     2161 tggtcgaggt ggtgccgcgc acgatcgagt cgtcgaccag caccacgcgc ttgcccgcca
     2221 gcaccgcccc gttggcgttg tgcttgagct tgacgccgag atggcggatc gagtcgctcg
     2281 gctcgatgaa ggtgcgcccg acatagtggt tgcggatgat gccgagctcg aaggggaggc
     2341 ccgcctgctc ggcatagccc atcgccgcgg gcacgcccga atccggcacc ggcaccacga
     2401 cgtcggccgg caccccgctc tcgcgcgcga gctcggcgcc gatccgcttg cgcgcctggt
     2461 agacgttgcg gccctcgacc acgctgtcgg ggcgcgagaa gtagatgtac tcgaagacgc
     2521 agaacttcga cggcgcgcgc gcgaacggct tgagcgtcct gacgccgtcg gcgtcgatca
     2581 ccaccacctc gccggcctcg acgtcgcgca cgtggcgggc gccgatgatg tcgagggcgc
     2641 aggtctccga ggtcaggatg tagccgccgg gccccgggtc aggcccgggg atgcgcccga
     2701 gcaccagcgg gcgcacgccg aagcggtcgc gcacgccgat catcgcctcg cccgtgagca
     2761 ggatcagcga gtaggcgccc tggacgtggc cgagcgcgtc gaccagccgg tcgagaaagc
     2821 cggtggcggc gctggtcgcg atcagatgga cgatgacctc ggtatccatg gtcgactgga
     2881 agatgcagcc gcgcttgacc agctcgcgcc tgagctggag ggcgttggtc aggttgccgt
     2941 tatgcgcgat cgccaggccg ccgccctcga ggtcggcgaa gatcggctgg acgttgcgca
     3001 gcatggtctc gccctgggtc gagtagcgga cgtggccgat cgcgcgctcg cccgccagcg
     3061 cggccatgac cgccggcgag ctgaagatgt cgccgacatg gccgaggcta cggtgggtat
     3121 ggaacacccg cccgtcgtag gacacgatgc ccgccgcctc ctggccgcgg tgctggaggg
     3181 cgtggaggcc gagcgcggtg tgcttggcgg cgtccgggtg cccgtacagg ccgaagatgc
     3241 cgcactcctc gcggagcttg tcgtcgtccc aggggtgcat cgtgctgcgc gccgccatct
     3301 ttgcccgagc cttattggct gccctggcct tcggccgcgg gcttgtcctt caggctgtcg
     3361 atcagccggt ccatctcctg ggactcggag tcgcggtagc ccgggtcgct cgccgtcggc
     3421 gcaccggccg cgaccggcgg gcgcgacagc tgctcgacct cgctcggcgt gccgggggaa
     3481 ccgttcccgc cggtcaggcc gcgcgcgttt cgcatctcac ttagcttctc tatcggcagg
     3541 ttgaggaagt cctttggggt cacggccacg atcagcccgg cggcgtagtc gatcagtggc
     3601 cggctgcgcg cgtcctcgag ccattcaggg cgcgggtggt ccgcgtcgtc ccacagcgtc
     3661 atctcgccga ccatgtaggc gagcgccacc accaccaccc cgcgcgcgac cccgaacagg
     3721 aagccgagcg agcggtcgag ggcattgagc gggctgtcct tgacccgtat cacgatcagg
     3781 tggatgacca gccacaaggc gaccagtgcg gccaggaaca ggccggcgcc ggtggcgacg
     3841 ttgaccaaaa attcgtccgc gatgtactcg gcgacatagc tgcgggcatc ggtgaagaaa
     3901 tagtacgtca ccaccgccgc gccgacccag ccggcgatcg ccagcgtctc gcgcacgaag
     3961 ccgcgataga acgcgaacac tgccgagacc agcaccacca gggcgacccc gagatcgacc
     4021 agattgaccg tttgctgctc catgcgcgac cgccttgtcc gggtccgggc tgcggtggcc
     4081 tagcgggcca gcgccgtgcg caccgccggc tcgccgaagg ccgcgatcag gtcggccagg
     4141 cgggcgatct cgaccagctc gagcgccgcc ttgaccgggc tgcgcgccgg cggcagccac
     4201 gcctgctcga agccgagttt cgccgcctcc ttcagccgcg cctcgctgtg cgccaccggc
     4261 cggacctcgc cggacagccc gatctcgccg aacacaatgg tgcgtccggg catcggcgta
     4321 tcggtgagcg aggacaccag cgccgccgct accgcgaggt ctgcggccgg ctcggcgatc
     4381 ctcaacccgc cggcgacgtt gagatatacg tcgcgggcgc cgaccgcaag gccgcagcgc
     4441 gcatcaagca ccgccagcac catggcaagg cgcgccgaat cccagccgat caccgcccgc
     4501 cggggggtgc cgaagggcga cggcgagacc agcgcctcga tctcgaccag catcggccgg
     4561 gtgccctcga cgccggcgaa caccgccgcg ccgctgacgg cttgcgcgcg ctcgctgagg
     4621 aacaggcgcg acgggttggc gacctcgcgc aagccgccgt cggtcatctc gaacacgccg
     4681 atctcgtcgg tggcgccgaa ccggttcttg accgcgcgca ggatgcggaa ctggtggccg
     4741 cgctcgccct cgaagtagag cacggtatcg accatgtgct cgagcacccg cggccccgcg
     4801 atctggccct ccttggtgac gtggccgacc agcaccagca ccaccccgcg ccgcttggcg
     4861 aggcggatca gctcctgggc gctggcgcgc acctgggcga ccgtgccggg cgccgaatcg
     4921 agggtgtcga catacatggt ctggatcgag tcgatgacca gcacctgggt cgcgtcggcg
     4981 tcgtcgagag tcgccaggat gtcgcgcacg ctggtcgccg ccgccagcga caccggcgcg
     5041 tccgcgatcc ccaggcggtg cgcgcgcagg cgcacctgct cctccgactc ctcgcccgag
     5101 acgaacagcg cgcgcgcgcc cgcgcgcgcc ggcgccgcca acgcctgcag catcagggtc
     5161 gacttgccga ccccgggatc gccgccgacg aggaccgtcg tgcccgcgac caggccgccg
     5221 ccgcacaccc ggtcgagctc ggcgatgccg gtggccaggc gcgcggtctg cggcgcggaa
     5281 tcggtcagcg cgctgagccg caggcgcttg cccttggcct tgccgaggcc cttcggcacc
     5341 gcctcgggcg ccgcctcctc gaccaccgtg ttccaggcgc cgcaggcctc gcaccggccc
     5401 gcccagcgcg gctgcgacgc gccgcaggcc tggcacacat agcggctcgc ggcggcgcgc
     5461 gtgcggggcg cgctcacagc gcgcggacct ccatcttgat cgggccctcg gcgcggccgt
     5521 ggacgaactg atcgacgaac gcgttgtcgc tcgaatcgat gttcgcggtc gggccgcacc
     5581 agatgatctt gcccttgtag agcatcgcga tccggtcgga gatcttgcgc gcgctggcca
     5641 tgtcgtgggt gatcgacagc gcggtggcgc cgagctcgcg cacgcattgg acgatcaggt
     5701 cgtcgatcac gtcgcccatg atcgggtcga ggccggtggt cggctcgtcg aagaagatga
     5761 tctcgggccg ggcggcgatg gcgcgggcga gcccgacccg cttacgcatg ccgcccgaca
     5821 gctcggccgg atagagctcg ccgacctcgc gcccgagccc gaccgcggcg agcgtctcgc
     5881 tagcgatccg cttggcctcg gcgcgcgcga ggccgcggcc ctggatcagg ccgaaggcga
     5941 cgttctgcca cacgctcagg ctgtcgaaca gggcgctgcc ctggaacagc atgccgaact
     6001 tctggttgat ctgttcgcgc tcggcgccag cgaggcggac cacgtcctcg ccgtcgatca
     6061 ggatgcgccc ggcctcgggc gtgatcaggc cgaggatgca tttgagcatc accgacttgc
     6121 ccgagcccga gccgccgatc accaccaggg attcgccggt ccgcacctcg aggtcgatgc
     6181 cgtcgagcac gatgttgtcg ccgaagcgct tgcacacccc gtcgagcaag atctttgccg
     6241 gggcattcat ttggcgaaga agacctcggt gatgaagtag ttggcggtca ggatcaggat
     6301 cgacgcggag accaccgcat tggtggtcgc ggtgccgacg ccttgcgcgc cgccgcggct
     6361 gtggtagccg tgatagcagc ccatcagggc gatgatcagg ccgaacgccg ccgccttgac
     6421 cagccctgag acgacgtcga gcgtctccag gttctcccag gtgctgtcga tgtagttggc
     6481 gccgttgaag ccgagcttgt tgaccccgac caggtagccg ccgagcacgc cgatgcagtc
     6541 ggcgaccagc accagcagcg gcagcatggt caccccggcg acgatccggg gcgcgaccag
     6601 gtacttgaac gggttggtcg acagcgtggt cagcgcgtcg atctgctcgg tcaccctcat
     6661 ggtgccgatc tcggccgcaa tcgaggcgcc gatgcgcccg gcgaccatca gcccggtcag
     6721 caccgggccg agctcgcggg tgatcgagat caccaccacc gacgggatcg cggtctcggc
     6781 gctgaaccgg ctgaagccgg tgtagctctg caaggcgagc accatgccgg cgaacaggcc
     6841 ggtgaggccg accacgggca gcgagtagta gccgatgtcg agcatctggc gcgcgatcag
     6901 gcgcggatag agcggcgggc ggacgcagtg gctgaccccg gtggcggcga acagggtcac
     6961 ccggccggtg gtctccatga acgcaaggaa cacccggccg atcagcgcca ggccgttcac
     7021 cgcgcgccct ccgcggcgtc ctcgacatag agccgcggca gccgggcgcc gagccgggtc
     7081 aagatctcat aggcgatggt gccggcctcg gcggcgacgt cgtcgagcgg gcgcgcaccg
     7141 ccgatcaggt cgacccaggc gcccagccgg gcgctgtccg ccggcacgtc cgagacgtcc
     7201 agtgtgatca ggtccatcga tactcggccc accacaggca ccaacaccgc gccctgcgcc
     7261 ggctccgcaa gcgccgcttg cgccaggttg ccgagcgcgc gcggaaagcc gtcggcgtaa
     7321 ccgaccggaa tggttgcgat ccggcccttg cgggccaacc gatgggtggc accgtagcca
     7381 acggtcatgg ccctgtcaac gtcgcgcagg gcgatgattt tcccttgcat gcgaactact
     7441 tgcgccatcg ggttggggcc ggcgggcgac gggttgatgc cgtagagcgc ggcgcccggg
     7501 cggaccatgt cgaagtggta agcggcgccg agcaggatgg ccgacgagtt ggcgaggctc
     7561 gcgcgcatcg ccggcaggcc ggcgatcgtt gccgcgaacc ggacgcgctg ggcggcgttc
     7621 atctggtgcc cggggttctc ggcgcaggcc aggtggctca agaccagctg gggcgcgaga
     7681 ccgctgagca ggtccggagc cccggcgagc cgccgcgcct cgcgctcgct cagcccgagg
     7741 cgcgacatgc cggtatcgag atggatcgcg gcgggaaggc tctgcgtgcg gcgcccggcc
     7801 tcgcccgccc agcgcgcgat cgcgccgagg tcgttcagca ccggcaccag cgccgccgcg
     7861 gcgaactcgc cctccgcgcc cggcatggcg ccgttgagca catagatcgc ggcctcgggt
     7921 acaagccgct tgagcccgag gccctcgtcg agggtggcga cgaagaagct ccggcagccg
     7981 gcggcggcga gcgccggcgc cacccgcgcc atgccgaggc cgtaggcgtc ggccttgacc
     8041 accgccgcgc actcgacccc ggcgccgatg cgcgccttga gcgcgcgcca gttgcgcgcg
     8101 atcgctgcca ggtcgatgat tagtcgggca ccggcccggt cggctgcgga caacatggcc
     8161 gctcgtttcc ccgagccgcc ccaccgcccg cgtcagtgcg tcgcctcggg caggtggtcg
     8221 gccggcgcat agctgtcgaa cttggtggtc tcggcggtga acgtcagctt gacgttgccg
     8281 atcgggccgt ggcgctgctt ggcgatcagc acgtcggcaa gcccggccgc cgactcgaag
     8341 cgctcctgcc agcgccggta gcgatcggcg aacctgtcgc ccgattcgtc ctggcgctgc
     8401 tgcggctcgg cgcgctcgag gtagtactcc tcgcggtaga cgaacatgac cacgtcggcg
     8461 tcctgctcga tggtccccga ttcgcgtaag tccgcgagct gcgggcgctt gtcctcgcgc
     8521 agctcgaccg cgcggctgag ctgggacagc gccagcaccg gcaggtccag gtccttggcc
     8581 agcgccttca gcccctgggt gatctccgag atctcctgga cccggctgtc gtggcggccg
     8641 gccggacgca tcagctgcag atagtcgacc accaccagcg acagcccgtg ggtgcgcttg
     8701 aggcggcggg cgcgcgtgcg cagcgccggc acgctcagcg ccggggtgtc gtcgatgaac
     8761 agcggcgcgg cgctgagcgc gcggctggcg tcgaccaggc gcatgaagtc gtcgttcgac
     8821 agcttgccgc ggcggatgtg gtcggagcgg atcttggccg cctcggcgag gatgcgcatc
     8881 gccagctgct cggccgacat ctcgagcgag aagaagccga ccaccgcgcc atcgaccggc
     8941 accggccgcc cggccgcgtc cgcctcggtg cggtaggtgc gggcggcgtg gaaggcgatg
     9001 gtggtggcga gcgcggtctt gcccatcgac ggcctgcccg ccaggatgac caggtccgaa
     9061 tcgtgcagcc cgcccagcag gttgtcgagg tcgatcaggc cggtggcgac cccggacagc
     9121 cggccctcgc gcttgtaggc ggcctcggcg ctgaggatcg cctcggtgag tgcgctcgac
     9181 agcggcttga acccgccctc ctcgcgcccg gtctcggcga gctcgtagag cttggcctcg
     9241 gcgccctcga tctgggccgc ggcgctgtcc tcgattcgcg ggtcgaaggc cgtatccacg
     9301 atctcgccgc cgatgtggat caggccccgg cgcaggctca ggtcgtggac cagccggccg
     9361 tagtcctcgg cgttgatcag gctgacggcg gcgccggcga ggcgggcgag gtactgcgcg
     9421 ccgccgatgt cggcaaggct ctcgtcggtc tcgaaatagg ccttcagcgt caccgggttg
     9481 gcgatctggc cgcggctgat gagtgcgcgc gcggcggcga agatgcggcc gtggacggga
     9541 acgaagaaat gctcgggctc gaggaaggtc gagaccgggt cgaaggcggc gttgtcgacc
     9601 aggacggcgc ccagcagcgc ctgctcggcc tggtcgttgt gcggcatctc gcggtagccg
     9661 agggtggccg tgtcgtcgcg ctcgggctgc gggtcgggta cgaagggcag ggggtccatg
     9721 cgccgcagtc taatgcgatc ggcgtcgatt gcacgtcccc gcgtctgtgc aacacgggga
     9781 acctggggat aaggccgcgc gcgatgtcgg gcttggcgcc ggctgcgggc aatgcgatca
     9841 tgcccgcgcg cgccgatggc tgagcggcgc gtggttgcag cgagggagag gcccatgaag
     9901 ctctacatgc atccggtgtc gacgaccagt cgcccgatcc tgctgttcat cgccgagaac
     9961 aagatcgacg tcgagctgca agtggtcgac ctgatgaccg gcgagcactt ccaggagccg
    10021 tacgtgcgga tcaatccgaa tcggctggtg ccggtgctcg aggacggcga cttccgactc
    10081 accgaaagct cggcgatcct caaatatctg gccgacaaga ttgattcgcc ggcctacccg
    10141 aaggacctgc ggaagcgcgc gcgggtgaac gaggtaatgg actggttcaa cagcaacttc
    10201 ggccgcgact actgctatgg cctggtctat ccgcagatct tcccgaacca caagcgcccg
    10261 gacgcgaccg tccaggcggc ggtgctcgcg cgcggcaagg aaggcgcgca gacctgg
//
LOCUS       DASWHU010000423         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2955391, whole genome shotgun sequence.
ACCESSION   DASWHU010000423 DASWHU010000000
VERSION     DASWHU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955391"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..>2744)
                     /gene="dnaE"
                     /locus_tag="VGB88_15360"
     CDS             complement(108..>2744)
                     /gene="dnaE"
                     /locus_tag="VGB88_15360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9648883.1"
                     /translation="FMVAARPPLLPASPKVAPGQSEAEALREIAEAGLEDRLARHVLR
                     PGADETARAAAAKPYRERLAYELGVISEMGFAGYFLIVADFIQWAKAQGIPVGPGRGS
                     GAGSVAAWALKITDLDPLRFGLLFERFLNPARISMPDFDIDFCQDRRDEVIHYVQREY
                     GHDRVAQIITFGKLQARAVLRDVGRVLQMPYGRVDKLCKLVPYNPANPVGLQEAIDGE
                     PRLREARDSEPEVARLLRIALQLEGLYRHASTHAAGVVIGDRPLVELVPLYRDPRSDM
                     PVTQFNMKYVELAGLVKFDFLGLKTLTVLDRAVRLLAKRGIELDLGDLPLDDAATYDM
                     MSRGETVGVFQLEGSGMRNLLRDARVDNIEDIIALVALYRPGPMENIPKYVGCKHGRE
                     EPEFLHETITPVTRDTYGVIIYQEQVMKIAQVFAGYDLGHADILRRAMGKKIRSEMAA
                     QRQSFIDGAMARGVERARASYVFDLVDKFAGYGFNKAHSAGYALIAYQTAYLKANHPV
                     EFLAASMSLDRGNTDKLNTFRQELARLGIPLLRPDINRSGGDFEVEPGEAGAGAIRYA
                     LAAIRNVGAGAVAQIVAERARGGRFKDLFEFARRVDAGALNKRQLENLAKAGAFDSLD
                     PNRAQMVAAADLLLRYAAAAKEARGSGQKSLFADQVDDSATPALPAVADWPMVDKLHA
                     EFDALGFYLSAHPLDAYGNSLEQLGIAGYLALVESGAGTGRIAGVVLGKQERSSQRGR
                     YAHVQMSDQSGLFELTVFAEQLASARDLIEPGRMLMVDVGIQRDGEVVRLTASRVRSL
                     DDSAARHAAGFRVFLSAEVSLAALQSVVARERTGAGRLALVVPFNGAGEVEIELGGGF
                     AVSPAARAALKAVPGVIDVHDL"
BASE COUNT          387 a          977 c          981 g          399 t
ORIGIN      
        1 cgcctgcccg ggccggaacg cgcggaagcc gaacacgccc tccagcaccc gctccgccgc
       61 cgccatattg gcgggctcgg accgcacaga gactgccgcc gcgcgcgtca caggtcgtgg
      121 acgtcgatca cgccggggac ggccttgagc gcggcgcggg cggcggggct caccgcgaag
      181 ccgccgccga gctcgatctc gacctcgccg gcgccgttga acggcaccac cagcgcgagc
      241 cggccggcgc cggtgcgctc gcgcgcgacc acgctctgca aggccgcgag cgacacctcg
      301 gccgacagga acacgcggaa gccggcggcg tggcgcgcgg cgctgtcgtc gagcgagcgc
      361 acccgcgagg cggtcaggcg caccacctct ccgtcgcgct ggatgccgac gtcgaccatc
      421 agcatccgcc cgggctcgat caggtcgcgg gcgctcgcga gctgctcggc gaacacggtc
      481 agctcgaaca gcccgctctg gtccgacatc tggacgtggg cgtaacgtcc gcgctgcgag
      541 ctgcgctcct gcttgccgag cacgaccccg gcgatgcggc ccgtgccggc gccgctctcg
      601 accagcgcca ggtagcccgc gatccccagc tgctcgagac tgttgccgta ggcgtcgagc
      661 ggatgggcgg acaggtagaa gccgagcgcg tcgaactcgg cgtgcagctt gtcgaccatc
      721 ggccagtcgg cgaccgcggg cagcgccggc gtcgcgctgt cgtcgacctg gtcggcgaac
      781 aggctcttct ggcccgagcc gcgcgcctcc ttggccgcgg ccgcatagcg cagcagcagg
      841 tcggcggcgg cgaccatctg ggcgcggttg ggatcgagcg agtcgaaggc gccggccttg
      901 gccaggttct cgagctggcg cttgttcagc gcgccggcgt cgacccggcg ggcgaactcg
      961 aacaggtcct tgaaccggcc gccgcgcgcg cgctcggcca cgatctgcgc caccgcgccg
     1021 gcgccgacat tgcggatcgc ggccagcgcg tagcggatcg cgcccgcgcc cgcttcgccg
     1081 ggctcgacct cgaagtcgcc cccggagcgg ttgatgtccg ggcgcaacag cgggattccg
     1141 aggcgcgcca gctcctggcg gaacgtgttg agcttgtcgg tattgccgcg gtcgaggctc
     1201 atcgaggcgg ccaggaactc gaccggatgg ttcgccttca ggtacgcggt ctgataggcg
     1261 atcagggcat agcccgccga gtgcgccttg ttgaacccgt agccggcgaa cttgtcgacc
     1321 aggtcgaaca cgtagctcgc gcgcgcgcgc tcgacgccgc gggccatcgc gccgtcgatg
     1381 aagctctggc gctgggccgc catctcggac ctgatcttct tgcccatggc gcggcgcagg
     1441 atgtccgcgt gccccaggtc gtagccggcg aacacctggg cgatcttcat cacctgctcc
     1501 tggtagatga tcaccccgta ggtgtcccgg gtcaccggcg tgatcgtctc gtgcaggaac
     1561 tcgggctcct cgcggccgtg cttgcagccg acatatttcg ggatgttctc catcggcccg
     1621 ggccggtaga gcgcaaccag cgcgatgatg tcctcgatgt tgtcgacgcg cgcgtcgcgc
     1681 agcaggttgc gcatgcccga accttcgagc tggaacacgc cgacggtctc gccgcggctc
     1741 atcatgtcgt aggtggcggc atcgtcgagc ggcaggtcgc cgaggtcgag ctcgatgccg
     1801 cgcttggcca gcagccgcac cgcccggtcg agcacggtca gcgtcttgag gccgaggaag
     1861 tcgaacttca ccagccccgc cagctcgaca tatttcatgt tgaactgggt caccggcatg
     1921 tccgagcgcg ggtcgcggta gagcggcacc agctcgacca gcgggcggtc gccgatcacc
     1981 acgcccgcgg cgtgggtcga ggcgtgccgg tagagcccct cgagctggag cgcgatgcgc
     2041 agcaggcggg cgacctcggg ctcgctgtcg cgcgcctccc tcagccgcgg ctcgccgtcg
     2101 atcgcctcct gcaggccgac agggttggcc gggttgtagg gcaccagctt gcacagcttg
     2161 tcgacccggc cgtagggcat ctgcaagacg cggcccacgt cgcgcagcac tgcgcgcgcc
     2221 tgcagcttgc cgaaggtgat gatctgggcg acccggtcgt ggccgtactc gcgctggacg
     2281 tagtggatca cctcgtcgcg ccggtcctgg cagaagtcga tgtcgaagtc gggcatcgag
     2341 atgcgcgccg ggttgaggaa gcgctcgaac aggagcccga agcgtagcgg gtcgaggtcg
     2401 gtgatcttga gcgcccaggc ggcgaccgag cccgcgcccg agccgcgtcc ggggccgacc
     2461 gggatacctt gcgccttggc ccactggatg aaatcggcga cgatcaggaa gtagccggca
     2521 aaccccatct cgctgatcac gccgagctcg taggcgagcc gctcgcgata gggcttggcc
     2581 gcggcggcgc gcgcggtctc gtccgcgccg gggcgcagca cgtgcctggc gagcctgtcc
     2641 tccagcccgg cctcggcgat ttcgcgcagc gcctcggcct cgctctgccc gggcgccacc
     2701 ttgggcgagg ccggcagcag cggcgggcgc gccgcgacca tgaa
//
LOCUS       DASWHU010000424         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_2582132, whole genome shotgun sequence.
ACCESSION   DASWHU010000424 DASWHU010000000
VERSION     DASWHU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582132"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VGB88_15365"
     CDS             complement(<1..177)
                     /locus_tag="VGB88_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648884.1"
                     /translation="MRLEGKIALISAAASGMGRAGARLFAAEGAKVVAADIDRAKVEA
                     VAKEIRGAGGEATAV"
     gene            428..1480
                     /locus_tag="VGB88_15370"
     CDS             428..1480
                     /locus_tag="VGB88_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX9648885.1"
                     /translation="MPAVLPRIVYFDHLQHRAGRELLETSPAVEFLRLERHAAPATIW
                     PVMAGAHGYQIASARQELPRHLHADADFLARCPELLVVSTDGAGADTVDNDACTGVGV
                     LVVNQAGGNREAVAEHALAMMLALVKRIGEADRALRRDRAWHRTDFWGRELHGRTLGI
                     VGLGHVGSRIAELCGGAFSMRVLAHDPYIGPERFARFAAEPVGLDELLARADVVSVNC
                     PLSAETRGMFDARAFARMKAGALFVTTARGGIHDEAALVEALRSGHLAGAGVDVWVEE
                     PPPIDHPLLAFDNVVATPHMAGVTEESRRRTSLICAEQWLAIWRGERPPRLLNADAWP
                     AYAARFRARFGLTVAA"
     gene            1484..2974
                     /gene="tcuA"
                     /locus_tag="VGB88_15375"
     CDS             1484..2974
                     /gene="tcuA"
                     /locus_tag="VGB88_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX9648886.1"
                     /translation="MADADVIVVGAGNAGMCAALAAAEAGAGVIVLERAHEDARGGNT
                     RFTAGAMRVVYDGVDDLLALMPDLGPEERRTTDFGSYPADRFFDDMARVTDFRANPDL
                     VEVLVHRSHPTLAWMRAHGIRFMPMYSRQAFKVGGRWRFWGGLTVEAYGGGPGLVEAE
                     FRAAGKAGIEVRYGARAMALVRDGDRVAGVRVRSGGEAAELGARAVVLACGGFEANAE
                     WRTRYLGPGWDLAKVRGTRFNTGDGIRMALDEGALAAGNWSGCHAVGWDRNAPAFGDL
                     AVGDGFQKHSYPFGIMVNAQGERFVDEGADFRNYTYAKYGRVVLQQPGQFAWQVFDAK
                     VDHLLRDEYRIREVTKAEAATLEDLAARLDGVDAAAFLATVRAFNAAVRTDVPFDPTV
                     KDGRGTMGLAVPKSNWANPLDSPPFKAFAVTCGITFTFGGIAITPEAEVIDADGGTIP
                     GLFAAGELVGELFYNNYPGGTGLTSGAVFGRIAGAGAARAALGRNR"
     gene            2971..3858
                     /locus_tag="VGB88_15380"
     CDS             2971..3858
                     /locus_tag="VGB88_15380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648887.1"
                     /translation="MTQAGTLGFIGLGVMGEPMCRNLAKRSGRAVIAHDVRPAPLARL
                     AQDGVGAARDVPSVAAAAEVVFLSLPDGAAVEAVCASLLEAEDAPLALVVDCSTAPVA
                     LARDLHRRFAARGIGFADAPVARTRAAAEAGTLAVMVGADEKTFAALRPLLACMASDV
                     LHCGEVGAGQATKILNNMVLFETVIALAEALAIARAAGFDGARLFEALTQGSADSFAL
                     RNHGRKALLAGDFPERAFSCRYALKDLGYALDLAREGGVDARAAECAQGVLEAAVEAG
                     YGDAYFPVVLKAIERAPQR"
     gene            complement(3849..>4055)
                     /locus_tag="VGB88_15385"
     CDS             complement(3849..>4055)
                     /locus_tag="VGB88_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9648888.1"
                     /translation="GAREGLNDWKRTGYGGPCPPIGRHRYFFKLYALDATLGDLRRPT
                     KAKLEAAIDGHVIARAELIGTYQR"
BASE COUNT          496 a         1492 c         1490 g          577 t
ORIGIN      
        1 gaccgcggtg gcctcgccgc cggcgcccct gatctccttc gccaccgcct cgaccttggc
       61 ccggtcgatg tcggccgcca ccaccttggc gccttcggcg gcgaacagcc tggcgccggc
      121 gcggcccatg ccggacgccg cggcgctgat cagcgcgatc ttgccttcga gcctcatcgg
      181 tccctccccg tcatcgatgg ttcgccagtg ctatgccccg cggccggccc gggcgcaagc
      241 ccgatccggg acgagcatgc gacggcgcga gaaagatttg ggcacgcgga ggcgcggaga
      301 acgatcacgc cctttctttc cccgcgacct ccgcgactcc gcgtgacccg aatggcgccc
      361 cattgcggcc cttgcccgga ggtggtgcag tgggcatggt agcggcccgc cttgccggag
      421 cctgctcatg cccgccgtcc tgccgcgtat cgtctacttc gaccacctcc agcaccgggc
      481 cgggcgcgag ctcctggaga cctcgccggc ggtcgagttt ttgcgcctcg agcgccacgc
      541 cgcgccggcg acgatctggc cggtgatggc cggggcccac ggctaccaga tcgcctcggc
      601 gcgccaggag ctgccgcgcc atctccacgc cgacgcggac ttccttgccc gctgccccga
      661 gctgctggtg gtgtcgaccg acggcgccgg cgccgacacg gtcgacaacg acgcgtgcac
      721 gggcgtgggc gtgctggtgg tcaaccaggc cggcggcaac cgcgaggcgg tggccgagca
      781 cgcgctggcg atgatgctcg ccttggtcaa gcgcatcggc gaggccgacc gggcgctgcg
      841 ccgcgaccgc gcctggcacc gcaccgactt ctggggccgc gagctccacg ggcgcacgct
      901 cggcattgtc ggcctgggcc atgtcggctc gcgcatcgcc gagctgtgcg gcggcgcgtt
      961 ttccatgcgc gtgctcgccc acgaccccta tatcgggccc gagcggttcg cccgcttcgc
     1021 cgccgagccg gtcgggctcg atgagctgct cgcgcgcgcg gacgtggtct cggtcaactg
     1081 cccgctcagc gccgagaccc gaggcatgtt cgatgcgcgc gcgttcgcgc gcatgaaggc
     1141 gggcgcgctg ttcgtcacca ccgcgcgcgg tggcatccac gacgaggccg cgctcgtcga
     1201 ggcgctgcgc agcggccacc tcgccggcgc cggcgtcgac gtgtgggtcg aggagccgcc
     1261 gccgatcgac cacccgctgc tcgccttcga caacgtggtg gcgaccccgc acatggccgg
     1321 cgtgaccgag gagtcgcgca ggcggacctc gctgatctgc gccgagcaat ggctcgcgat
     1381 ctggcgcggc gagcgcccgc cgcggctgct caacgcggac gcctggccgg cctatgcggc
     1441 gcggttcagg gcgcgcttcg gcctgaccgt cgccgcctga gcgatggcgg acgccgacgt
     1501 catcgtggtc ggcgccggca acgccggcat gtgcgccgcg ctcgccgccg ccgaggccgg
     1561 cgcgggcgtg atcgtgctcg agcgcgcgca tgaggatgcg cgcggcggca acacccgctt
     1621 caccgccggc gccatgcggg tggtctacga cggcgtcgac gacctgctcg cgctgatgcc
     1681 ggaccttggg ccggaggaac ggcgcacgac cgacttcggc agctatcccg ccgaccggtt
     1741 cttcgacgac atggcgcgcg tgaccgactt ccgcgccaac ccggacctgg tcgaggtgct
     1801 ggtgcaccgg agccacccga cgctcgcctg gatgcgcgcc cacggcatcc gcttcatgcc
     1861 catgtacagc cgccaggcgt tcaaggtggg tggccgttgg cggttctggg gcgggcttac
     1921 ggtcgaggcg tatggcggcg gccccgggct ggtcgaggcc gagttccgcg ccgcgggcaa
     1981 ggccggaatc gaggtccgct acggcgcgcg cgcgatggcg ctggtccgcg acggcgaccg
     2041 ggtcgcgggc gtgcgcgtgc gctccggcgg cgaggcggcc gagctcggcg cgcgcgcggt
     2101 ggtgctcgcc tgcggcggct tcgaggccaa tgccgagtgg cgcacgcgct atctcggccc
     2161 cggctgggac ctcgccaagg tgcgcggcac ccgcttcaac accggcgacg gcatccgcat
     2221 ggcgctcgac gagggcgcgc tcgcggccgg caactggtcg ggctgccacg cggtcggctg
     2281 ggaccgcaac gcgccggcgt tcggcgacct cgcggtcggc gacggcttcc agaagcactc
     2341 ttacccgttc ggcatcatgg tcaatgcgca gggcgagcgc ttcgtcgacg agggcgccga
     2401 cttccgcaac tacacctacg ccaagtacgg ccgcgtcgtc ctgcagcagc ccggacagtt
     2461 cgcctggcaa gtgttcgacg ccaaggtcga ccacctgctg cgcgacgagt accgcatccg
     2521 cgaggtgacc aaggccgagg cggcgacgct cgaggacctg gccgcgcgcc tggacggggt
     2581 cgacgccgcg gcgttcctcg ccacggtgcg ggcgttcaac gccgccgtcc gcaccgacgt
     2641 gccgttcgac ccgacggtca aggacggccg cggcacgatg ggcctcgcgg tgcccaagag
     2701 caactgggcg aacccgctgg attcgccgcc gttcaaggcg ttcgcggtga cctgcggcat
     2761 cacgtttacc ttcggcggca tcgcgatcac gcccgaggcc gaggtgatcg acgccgacgg
     2821 cggcaccatc cccggcctgt tcgccgccgg cgagctggtc ggcgagctgt tctacaacaa
     2881 ctatccgggc ggcaccggtc tcacctcggg cgccgtgttc gggcgcatcg caggtgcggg
     2941 cgcggcgcgc gcggcgctgg gtcgcaaccg atgacgcaag cgggcaccct cggattcatc
     3001 ggcctcggcg tgatgggcga gccgatgtgc cgcaatctcg ccaagcggtc ggggcgggcg
     3061 gtgatcgccc acgacgtgcg gccggcgccg ctcgcgcggc tggcgcagga cggcgtgggc
     3121 gcggcgcgcg acgtgccgtc agtcgccgcc gccgcggagg tggtgttcct gtcgctgccc
     3181 gatggcgcgg cggtcgaggc ggtgtgcgct tcgctactcg aagcggagga cgcgccgctc
     3241 gcgctggtcg tcgactgctc gaccgcgccg gtcgcgctcg cccgcgacct ccaccgccgg
     3301 ttcgccgcgc gcggcatcgg cttcgccgac gcgccggtcg cgcgcacccg cgccgccgcc
     3361 gaggccggta cactcgcggt catggtgggg gcggacgaga agaccttcgc cgcgctcagg
     3421 ccgctgctcg cctgcatggc aagcgacgtg ctccactgcg gcgaggtcgg cgccggccag
     3481 gcgaccaaaa tcctcaacaa catggtgctg ttcgagaccg tgatcgcgct ggccgaggcc
     3541 ttggcgatcg cgcgcgccgc ggggttcgac ggcgcgcggc tgttcgaggc gctgacccaa
     3601 ggctcggccg acagcttcgc gctgcgcaat cacggccgga aggcgctgct ggcgggcgac
     3661 ttcccggagc gcgcgttctc gtgccgctac gcgctcaagg acctcggcta cgcgctcgac
     3721 ctcgcgcgcg agggcggcgt cgatgcgcgc gccgccgagt gtgcgcaggg cgtgctcgag
     3781 gcggcggtcg aggccggcta cggcgacgcc tacttcccgg tggtgctgaa ggcgatcgag
     3841 cgcgcgcctc agcgctgata ggtgccgatc agctccgcgc gcgcgatcac gtgcccgtcg
     3901 attgccgcct cgagcttggc cttggtcggc ctgcggaggt cgccgagcgt ggcatcgagc
     3961 gcatacagct tgaagaagta gcggtggcgc ccgatcggcg ggcacggccc gccatagccg
     4021 gtgcgcttcc agtcgttgag cccctcgcgc gcgcc
//
LOCUS       DASWHU010000425         9564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_3657187, whole genome shotgun sequence.
ACCESSION   DASWHU010000425 DASWHU010000000
VERSION     DASWHU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3657187"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..879)
                     /locus_tag="VGB88_15390"
     CDS             complement(1..879)
                     /locus_tag="VGB88_15390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX9648889.1"
                     /translation="MATRSAPVSTVLGDAAGALVRLARRQPMLSREEERDLARRARRG
                     DRDAVSRLVKSHLRLVIGIAERYRAPGLHKGDLIQEGCIGLMQALQRFNPDHEARLAT
                     YARLWIKSAIQDHVVRSWSLVRLSTSTAQKSLFFNLRRMRAALSETGESLSESAVRHV
                     AAALNQTEAEVRRMARRLVGPDLSLDVAAGDQAGASSWVSQLADDAPTPEDAAIRRDE
                     TRRWTGALAAALGELRDRERLVIRRRYLSDVATTLEGLGRELGISKERVRQIEARAMA
                     KLRRILSQRVGEQATV"
     gene            940..1569
                     /locus_tag="VGB88_15395"
     CDS             940..1569
                     /locus_tag="VGB88_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9648890.1"
                     /translation="MVKKAEIRGHVLDAALKLAAERGWRRLRLADIAVAAGLPLATVA
                     ETFGSKAEILSAFSRRIDLAVLQGSDLADLDQPVRDRLFDVIMRRLDALKPHRAALGA
                     IAHDTARDPVAGACAGAVLVRSMAAMLEAAGVSASGLGGLIRAEGLAAIYLQVLRVWL
                     GDESEDLSKTMAALDRRLDRADWFMARLCGVSRRRPAAPAAAAATGEAQ"
     gene            1566..1826
                     /locus_tag="VGB88_15400"
     CDS             1566..1826
                     /locus_tag="VGB88_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648891.1"
                     /translation="MTGRAPDLGALLALTRGAFEAGDGASVMGRAMAGAWLEAEHIAQ
                     AAGAEAERAQLDAARAMFAVALARTRALALAALAAPPGAGKA"
     gene            complement(1994..3310)
                     /locus_tag="VGB88_15405"
     CDS             complement(1994..3310)
                     /locus_tag="VGB88_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648892.1"
                     /translation="MTRLRDFLPRDLFGRSLLIVTVPMVLLQAVSAYIFYTRHWEDVG
                     RRLALGLAGDIALMIDALEERHAAVQSQWLIRRARYNLAMDATFQQDARLPAQPPHDL
                     FGPVDWTLARVLPERIRYPFQFDTKHQPELVKISIQLPNGLLQVVVPRKRLFSSTTYV
                     FVLWMAGTALVLLAIAVYFLRRQIGPMRRLAHAADSFGKGIQVIDFKPQGATEVRQAA
                     HAFLRMRERIQRQMVQRTEMLAGVSHDLRTPLTRMKLQLAMGLEPGAVEALKADVADM
                     ERMVEGYLAFARGEGSEPAREVDIAGLLGDVVDGARRKDVAIALDGVPEGLSLTLRRD
                     AIKRALTNLIDNAARYGKSVGVACRRTARAIEITVDDDGPGIAPEHWESVFKPFYRLE
                     GSRNPDTGGVGLGLSIARDLVRNHGGDLVLAQSPQGGLRAIVQLPL"
     gene            complement(3307..4059)
                     /locus_tag="VGB88_15410"
     CDS             complement(3307..4059)
                     /locus_tag="VGB88_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9648893.1"
                     /translation="MTIDARAPGVPGPDGGPPHVLVVDDDQRLRELLRRYLSDNGFLV
                     ATAANAGEARARLKGLRFDLLVLDIMMPGEDGLSLARSLRAQDDVPILMLTAMDSVDD
                     RIQGLETGADDYLVKPFEPRELLLRLNAILRRTHSPEPAAPTAVRFGEFSFDLASGAL
                     RKGEAPVALTSAEEALLRALAGASGRPLARDALAARGHPGARALDVQITRLRRKLEPD
                     PRNPRHLVTVRGEGYALRAAPDADRRADEVTR"
     gene            complement(4124..4609)
                     /locus_tag="VGB88_15415"
     CDS             complement(4124..4609)
                     /locus_tag="VGB88_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9648894.1"
                     /translation="MTDLKAPVNPLFLREQDLNRGIELLYFGYRDFVAEPDRILAPLG
                     FGRAHHRVIYFIGRNADISVAQLLDVLQITKQSLSRVLGQLVRTGYVRQRQGTRDRRQ
                     RLLSLTDKGRELERRLSDTQRTRIARAYRAAGAEAVVGFQNVMLGLMTADDRGRFETG
                     D"
     gene            4699..5601
                     /locus_tag="VGB88_15420"
     CDS             4699..5601
                     /locus_tag="VGB88_15420"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX9648895.1"
                     /translation="MIDFDKRDGWIWYDGEMVPWRDAKLHVLSHGLHYASCVFEGQRV
                     YGGTIFRLEQHTERLFESARQLGFKLPFTVEEINQANYQVCRANTIADGYVRPVAWRG
                     SEMMGVSAQKNAIHVALAAWEWPSYFSPEARLKGLKLQWSKWKRPDPATIPAKAKAAG
                     LYMICTLSKHAAESDGFDDALMLDWRGRLAESTGANIFLVIDGALHTPTPDCFLDGIT
                     RQCVIALAKKRGIEVVERVIMPEELDKADEVFLTGTAAEVTPVGQIGDLHFQPGAMCR
                     LMIEDFTALTREPTPAPAHIGAGG"
     gene            complement(5808..6200)
                     /locus_tag="VGB88_15425"
     CDS             complement(5808..6200)
                     /locus_tag="VGB88_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9648896.1"
                     /translation="MPPRRSSRSLIGLLALVAGIVGEFAAEAGAGERNPYTGDQAAIA
                     DGAKLWAETGCYSCHGENAEGAVGPDLTDDQWVYRPTDATLFRTIAKGRSGTNMVGWS
                     GSLDDDEIWKIIAFIRSRYRGDPDKVTW"
     gene            complement(6309..8024)
                     /locus_tag="VGB88_15430"
     CDS             complement(6309..8024)
                     /locus_tag="VGB88_15430"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX9648897.1"
                     /translation="MTKRQLVHTGATCLALGAGVLVAGPAAAQDYVTDEMLLEAGQDP
                     NNWLMAPRDYTGSRYSPLRQVNVETIGRLVPKWTFSFGVLDAQNTTPLVNNGVMYATA
                     SHGRTYAIDARTGAEIWRYVDELPEGVAGKMCCDIGNRGAALYRDKVYVATPDADVVA
                     LDAKTGAVVWQVNIANWEESYTMTVAPLVVKGKVIVGLSGAEYPTRLFIEALDAETGR
                     QAWRRYTVPGPGEPGHDSWGSTDSAMFGGASAWITGSYDPELDTLYWGVGNPNPDWDG
                     VNRPGDNLYSNATLALDPDSGEIKHYFQYTPHDVWDYDGNNEPVLLDVDGRKLWLHGD
                     RNGFLYAIDRTNSKFVYGVPISKVNWATGFTAEGRPVVNADKVPAYDYEAQDICPASE
                     GGKWWNPMAVNPHTKMVFVPSREICTDIKSAPLGEGLNPAERTPGKPYWGIGTIKWNQ
                     GYGQLVAFDARTGDKVWTVKAPAPFTSGLMTTGGGLVFAGTPEGQFKAYDQDTGEELW
                     SYNVGTGIFGSPMTYAIDGKQYVAVPAGFGGWTGWATLGGGGAPHLKDSRKGGYLMVF
                     GLFGD"
     gene            complement(8244..9215)
                     /locus_tag="VGB88_15435"
     CDS             complement(8244..9215)
                     /locus_tag="VGB88_15435"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX9648898.1"
                     /translation="MTNRLCVGTCGLSVWFSDDLGETWTRPYSESGLYLEARVWALSW
                     HPGEPDVVYAGTDSGLYRWTDEDERWTHLPSPMDATPIWSLVQAPHDPALMFAGAQPA
                     ALYRSRDGGLSWQKLDAALPQDCMFVHSPRVTQIVFDPLDDQTLWAGVEIGGVWKSTD
                     EGETWRPCNAGLVSEDIHGLAVIANGGRKLFATTNKGLHVSTDDGATWHHQTLDSPWQ
                     YTRAIQPIGNGGTRLLLANGNGPPGSTGRLLDSTDAGERWRDANLPGTLNSTPWCIAA
                     NDADSELMFVLTNLGQLFRSTDGGHQWTKLAREFGEVRAALWHPLAA"
     gene            complement(9241..>9564)
                     /locus_tag="VGB88_15440"
     CDS             complement(9241..>9564)
                     /locus_tag="VGB88_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9648899.1"
                     /translation="EHYPGMTERKLAEIEAEARARWPLEDSLIIHRIGRLEPGERIVL
                     VAVASAHRHAALEACQFLIDWLKTEAPFWKREEDAHGARWVEARDADDAAAARWQADK
                     KHAAE"
BASE COUNT         1332 a         3347 c         3411 g         1474 t
ORIGIN      
        1 tcagaccgtc gcctgctcgc cgacgcgctg gctgaggatg cggcgcagct tggccatcgc
       61 ccgcgcctcg atctggcgca cccgttcctt cgagatgccg agctcgcggc cgagcccctc
      121 gagcgtggtc gccacgtccg acaaatagcg ccggcggatc accaggcgct cgcggtcgcg
      181 cagctcgccg agcgcggcgg ccagcgcccc ggtccagcgc cgggtctcgt cacggcggat
      241 cgccgcgtcc tcgggcgtcg gcgcatcgtc cgccagttgc gacacccagc tcgacgcgcc
      301 cgcctggtcg cccgcggcga cgtcgagcga cagatcgggc ccgaccaggc gccgggccat
      361 gcgccgcacc tccgcctcgg tctggttgag cgcggccgcg acgtggcgga ccgcgctctc
      421 gctcaggctc tcgccggtct cgctgagcgc cgcgcgcatg cgcctgaggt tgaagaacag
      481 cgacttctgg gcggtcgagg tgctgaggcg gaccagcgac cacgagcgca ccacgtggtc
      541 ctggatcgcc gacttgatcc acaaccgggc ataggtggca aggcgggcct cgtggtcggg
      601 gttgaagcgt tgcagcgcct gcatcaggcc gatgcagcct tcctggatca ggtcgccctt
      661 gtgcagcccg ggggcgcggt agcgctcggc gatcccgatc accagcctga ggtggctctt
      721 gaccaggcgc gagacggcgt cgcggtcgcc gcgccgggcg cgccgggcga ggtcgcgctc
      781 ctcctcgcgg ctgagcatcg gctggcggcg cgccaggcgc accagcgcgc cggcggcgtc
      841 gccgaggacc gtactgaccg gggccgaccg ggttgccatg aaaacaagca ttgcggcagg
      901 aggcggtgag tcaatatcat tttactcggg catcggcaga tggtgaagaa ggctgagata
      961 cggggccacg tcctggacgc cgccctcaag ctggcggcgg agcggggatg gcgacggctt
     1021 cgcctggccg acatcgcggt cgcggccggg ctgccgctgg cgacggtggc cgagaccttt
     1081 ggctccaagg ccgagatcct gtcggctttc agccggcgca tcgacctggc ggtgcttcag
     1141 ggtagcgacc tcgccgatct cgaccagccc gtccgcgacc gcctgttcga cgtgatcatg
     1201 cgccggctcg atgcgctcaa gccgcaccgg gcggcgctcg gcgcgatcgc ccacgacacc
     1261 gcccgcgacc cggtcgcggg cgcttgcgcg ggcgcggtgc tggtgcgctc gatggcggcg
     1321 atgctcgagg cggcgggggt ctcggcaagc ggactcggcg gcctgatacg ggccgagggc
     1381 ctggcggcga tctacctgca ggtgctgcgc gtctggctcg gcgacgagtc cgaggacctg
     1441 agcaagacca tggccgcgct cgaccgccgg ctcgaccgcg ccgactggtt catggcgcgc
     1501 ctgtgcggcg tgagccgccg ccgacccgcc gcccccgccg ccgccgcggc gacaggcgag
     1561 gcccaatgac cgggcgcgcg cccgacctgg gcgcgttgct cgcgctcact cgcggcgcat
     1621 tcgaagccgg ggacggcgcc tcggtcatgg ggcgggcgat ggcgggcgcg tggcttgagg
     1681 ccgagcatat cgcgcaagcg gccggggccg aggcggagcg ggcgcagctc gacgccgcgc
     1741 gggcgatgtt cgccgtggcg ttagcccgga cgcgcgcgct ggcgctggcc gcgctcgccg
     1801 caccgcccgg cgccggcaaa gcttagcccg gcaatcatcg cccccggcca cccggccgtg
     1861 ccgaacggct gcgattcgga acaacgaagt tcggcggccc gcgttgtcct tcgagacgcc
     1921 cgccttcgcc attggctacg gcgggcatct cgaagcacgt ggtgtgcgac ccacgctccg
     1981 atgatggccg gcactacagc ggcagctgga cgatcgcgcg cagtccgccc tgcggcgact
     2041 gcgcgagcac caggtcgccg ccgtggttgc gcaccaggtc gcgggcgatg ctcaagccca
     2101 gcccgactcc gccggtgtcc gggttgcgcg agccctcgag gcggtagaac ggcttgaaca
     2161 cggattccca atgctcgggc gcgatgccgg ggccgtcgtc gtcgaccgtg atctcgatcg
     2221 cgcgcgcggt tcgccggcag gccaccccga cgctcttgcc gtagcgcgcc gcgttgtcga
     2281 tcaggttggt cagcgcgcgc ttgatcgcgt cgcgcctgag ggtgaggctg agcccctcgg
     2341 gcacgccgtc gagcgcgatg gccacgtcct tgcgccgggc gccgtcgacc acgtcgccga
     2401 gcaggccggc gatgtcgacc tcgcgcgccg gctcgctgcc ctcgccgcgg gcgaaggcga
     2461 gatagccctc gaccatccgc tccatgtcgg cgacgtcggc cttgagcgcc tccaccgcgc
     2521 ccggctccag gcccatcgcg agctgcagct tcatgcgcgt cagcggcgtg cgcaggtcgt
     2581 ggctgactcc ggccagcatc tcggtccgct gcaccatctg gcgctggatg cgctcgcgca
     2641 tgcgcaggaa cgcgtgcgcc gcctggcgca cctcggtcgc gccctggggc ttgaagtcaa
     2701 tcacctggat gcccttgccg aagctgtcgg cggcgtgcgc gaggcgccgc atcggcccga
     2761 tctgtcggcg caggaagtag accgcgatcg cgagcagcac cagcgccgtg ccggccatcc
     2821 acagcacgaa cacataggtg gtcgagctga acagccgctt gcgcggcacc accacctgca
     2881 gcaggccgtt gggcagctgg atcgagatct tgaccagctc gggttggtgc ttggtatcga
     2941 actggaacgg gtagcggatg cgctcgggca ggacgcgggc gagcgtccag tcgaccggcc
     3001 cgaacaggtc gtggggcggc tgggcgggca ggcgcgcgtc ctgttggaag gtcgcatcca
     3061 tcgccaggtt gtagcgcgcc cggcggatca gccattgcga ctggaccgcg gcgtgcctct
     3121 cctcgagcgc gtcgatcatc agcgcgatgt cgccggccag gccgagcgcc agcctgcggc
     3181 ccacgtcctc ccaatggcgg gtgtagaaga tgtaggcgga aaccgcttgc agcagcacca
     3241 tcggcacggt cacgatcagc agcgagcggc cgaacaggtc gcgcggcaga aagtcgcgca
     3301 ggcgcgtcat cgcgtcacct cgtcggcccg gcggtccgcg tccggggccg ccctgagcgc
     3361 atagccttcg ccgcgcacgg tcaccagatg gcgcgggttg cgcgggtcgg gctcgagctt
     3421 gcggcgcagg cgcgtgatct gcacgtcgag cgcgcgcgcc cccggatgcc cgcgcgcggc
     3481 cagggcatcg cgcgccagcg gccgcccgga cgcgccggcg agcgcgcgca gcagcgcctc
     3541 ctcggccgag gtcagcgcca ccggcgcctc gcccttgcgc agcgcgccgc tggcgaggtc
     3601 gaagctgaac tcgccgaagc gcaccgccgt cggtgccgcc ggctccgggc tgtgggtgcg
     3661 gcgcaggatg gcgttcaggc gcagcagcag ctcgcgcggc tcgaacggct tgaccaggta
     3721 gtcgtcggcg ccggtctcca ggccctggat gcggtcgtcg accgagtcca tcgcggtcag
     3781 catcaggatc ggcacgtcgt cctgcgcgcg cagcgagcgg gcgagcgaca ggccgtcctc
     3841 gccgggcatc atgatatcga gcaccagcag gtcgaagcgc aggcccttga gccgcgcgcg
     3901 cgcctcgccg gcattggccg cggtcgcgac caggaagccg ttgtccgaca ggtagcggcg
     3961 cagcagctcg cgcagccgct ggtcgtcgtc gacgaccagc acgtgcggcg ggccgccgtc
     4021 cgggccgggc acgcccggcg cgcgcgcatc gatagtcatg ggccgattgt acgggctcgc
     4081 ccggccgcac ggaatggctt gcccgagcgg ggtctccgca gggtcagtcg ccggtctcga
     4141 agcgcccgcg gtcgtcggcg gtcatcaggc ccagcatcac gttctggaac ccgaccacgg
     4201 cctcggcgcc ggcggcgcga taggcgcggg cgatgcgcgt gcgctgggtg tccgacaggc
     4261 gccgctcgag ctcgcggcct ttgtcggtca ggctcagcag ccgctggcgc cggtcgcggg
     4321 tgccctggcg ctggcggaca tagccggtgc gcaccagctg gccgagcacc cggctcaggc
     4381 tctgcttggt gatctggagc acgtcgagca gctgggcgac cgagatgtcg gcgttgcggc
     4441 cgatgaagta gatcacccgg tgatgggcgc ggccgaagcc gagcggggcc aggatgcggt
     4501 cgggctcggc gacgaagtcg cggtagccga aatacagcag ctcgatgcct cggttgaggt
     4561 cctgctcgcg caggaacagc gggttcaccg gcgcctttaa gtcagtcatg ttgacacata
     4621 tgcgggcagg tgttagccaa tgcaaccccg atcttatacg atatccaggg gcgcggggct
     4681 gttcgagggc aggcgggaat gatcgacttc gacaagcgcg acggctggat ttggtacgac
     4741 ggcgagatgg tgccctggcg cgacgccaag ctgcacgtgc tgtcccacgg cctgcactac
     4801 gcgagctgcg tgttcgaggg ccagcgcgtt tacggcggca ccatcttcag gctcgagcag
     4861 cacaccgagc gcctgttcga gtccgcgcgc cagctcggct tcaagctgcc gttcacggtc
     4921 gaggagatca accaggccaa ctaccaggtc tgtcgcgcca acaccatcgc cgacggctac
     4981 gtccgcccgg tcgcgtggcg gggcagcgag atgatgggcg tctcggcgca gaagaacgcc
     5041 atccacgtcg cgttggcggc ctgggaatgg ccgtcctact tctcgcccga ggcgcggctc
     5101 aagggcctca agctgcagtg gtcgaagtgg aagcgccccg accccgcgac catcccggcc
     5161 aaggccaagg cggccgggct gtacatgatc tgcaccttga gcaagcacgc cgccgaatcc
     5221 gacggcttcg acgacgcgct gatgctcgac tggcgcgggc ggctcgcgga gtcgaccggc
     5281 gccaacatct tcctggtgat cgacggcgcg ctgcacaccc ccacccccga ctgcttcctc
     5341 gacggcatca cccgccagtg cgtgatcgcg ctggcgaaga agcgcggcat cgaggtggtc
     5401 gagcgggtga tcatgcccga ggagctcgac aaggccgacg aggtgttcct gaccggcacc
     5461 gcggccgagg tcaccccggt cggccagatc ggcgacctcc atttccagcc cggcgcgatg
     5521 tgccggctga tgatcgagga cttcaccgcg ctcacccgcg agcccacgcc cgcgcctgcg
     5581 cacatcggcg ccggcgggta gtacccggga ccgcaggcgt gtcgatcacg caggcgcggt
     5641 cgttgcgagc ccccgataca ggcgtgcgcg aagcgatcca gggtcggcgc gccactctgg
     5701 atcgccgcga ccgcttcgcg gtttcgcgat gatgccgttg cgcggtttgc gtatgcgtgg
     5761 gtgccggcag catgcgccgg cgaacgctgt tcgccagggc ccggcagcta ccaggtgacc
     5821 ttgtccgggt cgccgcgata gcgcgagcgg atgaaggcga tgatcttcca gatctcgtcg
     5881 tcgtcgagcg agcccgacca gccgaccatg ttggtgccgc tgcgcccctt ggcgatggtg
     5941 cggaacagcg tcgcgtcggt cgggcgatag acccattggt cgtcggtcag gtccgggccg
     6001 accgcgcctt cggcgttctc tccgtggcag ctgtagcacc ccgtctcggc ccacagcttg
     6061 gcgccgtcgg cgatcgcggc ctggtccccg gtatagggat tcctttcgcc cgcgccggcc
     6121 tcggcggcga attcaccgac gatgcccgcg accagggcga gcaggccgat caacgaccgg
     6181 gaagaacgcc gaggtggcat gacaggcttc cggtaacttt ggcggagctg agaaaactgg
     6241 gggagcggcc ggcgctcccc caaccggtgt cgtgaccagc cggcgtcgat gccggcccgc
     6301 tcgatcggtc agtcgccgaa caggccgaac accatcaggt atccgccctt gcggctgtcc
     6361 ttgagatggg gcgcaccgcc gccgccaagg gtcgcccacc cggtccagcc gccgaagccg
     6421 gccggcaccg cgacatattg cttgccgtcg atggcgtagg tcatggggct gccgaaaatg
     6481 ccggtgccga cattgtacga ccacagctcc tcgccggtgt cctggtcata ggccttgaac
     6541 tggccttccg gcgtgccggc gaacacgaga ccgccaccgg tggtcatcag gccgctggtg
     6601 aacggcgccg gcgctttgac ggtccagacc ttgtcgccgg tacgcgcatc gaacgcgacc
     6661 agctggccgt agccctggtt ccacttgatg gtgccgatgc cccaataggg cttgccgggg
     6721 gtgcgctcgg ccgggttcaa cccttcgccc agcggcgcgc tcttgatgtc ggtgcagatc
     6781 tcgcggctgg gaacgaacac catcttggtg tgcggattga ccgccatcgg gttccaccac
     6841 ttgccgccct ccgacgccgg gcagatgtcc tgggcctcgt agtcgtaggc cggcaccttg
     6901 tccgcgttga ccaccggccg gccctcggcg gtgaagccgg tcgcccagtt caccttcgag
     6961 atcggcacgc catagacgaa cttgctgttg gtgcggtcga tcgcgtacag gaagccgttg
     7021 cggtcgccgt gcagccacag cttgcggccg tcgacgtcga gcagcaccgg ctcgttgttg
     7081 ccgtcgtagt cccagacgtc gtgcggcgtg tactggaagt agtgcttgat ctcgccgcta
     7141 tccgggtcga gcgcaagggt ggcgttcgag tacaggttgt cgcccggccg attgacgccg
     7201 tcccagtccg gattgggatt gccgacgccc cagtagaggg tgtcgagctc ggggtcgtac
     7261 gagccggtga tccacgccga ggcgccgccg aacatcgccg aatcggtgct gccccagctg
     7321 tcgtggcccg gctcgcccgg gcccggaacg gtgtagcgcc gccatgcctg ccggccggtc
     7381 tcggcatcca gcgcctcgat gaacaggcgc gtcggatact cggcgcccga caagccgacg
     7441 atgaccttgc ccttcaccac cagcggcgcg acggtcatgg tgtaggactc ttcccagttg
     7501 gcgatgttga cctgccagac gaccgcgccg gtcttggcgt cgagcgcgac cacgtcggca
     7561 tcgggggtcg ccacgtagac cttgtcccgg tacagcgcgg cgccgcggtt gccgatgtcg
     7621 cagcacatct tgccggcgac gccttccggc aactcgtcga catagcgcca gatctcggcg
     7681 ccggtacgcg cgtcgatggc gtaggtgcgc ccgtgcgagg cggtggcgta catgacgccg
     7741 ttgttgacca gcggcgtcgt gttctgggcg tcgagcacgc cgaacgagaa cgtccacttg
     7801 ggtaccagcc ggccgatggt ctcgacgttc acttggcgca gcgggctgta gcggctgccg
     7861 gtatagtcgc gcggagccat cagccagttg ttcgggtctt ggccggcctc gagcagcatc
     7921 tcgtcggtga cgtagtcctg ggccgccgcc gggcccgcga cgagcacgcc ggcgccgagc
     7981 gcgagacacg tcgcgcccgt atggaccaac tgccgtttgg tcataatgtt ccctccccta
     8041 tgtgcaacag cggcctcgcg cgtcggcgga agccccccgg agcgcctgac cgttcgtctg
     8101 acgatcctat cccatcaccg agccgtcgcc cacccggttc ggcggtcgca ggttctgcag
     8161 ggaggcgcgc gaacgcgcgc gctcgagtct cgcccgagcc ccgtgatcga ccggaccggc
     8221 tcggatcggt agtcgggtcc ggctcaggcc gcgagcgggt gccagagcgc ggcgcggacc
     8281 tcgccgaact cgcgtgcgag cttggtccac tggtggccgc cgtcggtgct gcggaacagc
     8341 tggccgagat tggtcagcac gaacatgagc tcgctatcgg catcgttggc ggcgatgcac
     8401 cagggcgtgc tgttgagcgt ccccggaagg ttggcgtcgc gccagcgctc gcccgcgtcg
     8461 gtgctgtcga gcagccggcc cgtcgagccc ggcgggccgt tgccgttggc gagcaggagc
     8521 cgggtgccgc cgttgccgat cggctggatc gcgcgcgtgt actgccacgg cgagtcgagc
     8581 gtttggtgat gccaggtcgc gccgtcgtcg gtgctcacat gcaggccctt gttggtggtc
     8641 gcgaacagct tgcgcccgcc gttggcgatc accgccaggc cgtggatgtc ctccgacacc
     8701 aggccggcgt tgcacggccg ccaggtctcg ccctcgtccg tgctcttcca caccccgccg
     8761 atctcgactc ccgcccacag cgtttggtcg tcgagcgggt cgaagacgat ctgggtgact
     8821 cgcgggctgt ggacgaacat gcagtcctgc ggcagcgcgg cgtcgagctt ttgccagctc
     8881 aggccgccgt cgcgcgagcg gtacagggcc gcgggctggg cgccggcgaa catcagggcg
     8941 ggatcgtgcg gcgcctggac cagcgaccag atcggcgtcg cgtccatcgg cgagggcagg
     9001 tgcgtccagc gctcgtcttc gtcggtccag cggtaaaggc cgctgtcggt gccggcgtag
     9061 accacgtccg gctcgcccgg gtgccagctc aacgcccaca cgcgcgcctc cagataaagg
     9121 cccgactcgc tgtagggcct ggtccaggtc tcgccaaggt cgtcgctgaa ccacaccgac
     9181 aagccgcagg tgcccacgca cagcctgttg gtcatctgtc gttcccccaa tccgctcgcg
     9241 ctattcggcg gcgtgcttct tgtccgcttg ccagcgcgcg gcggcggcat cgtccgcgtc
     9301 gcgcgcttcg acccagcgcg cgccgtgcgc gtcttcctcg cgcttccaga acggcgcctc
     9361 ggtcttcagc cagtcgatca ggaactggca cgcctcgagc gcggcgtggc ggtgggcgga
     9421 ggcgacagcc accagcacga tccgttcgcc cggctcgagc cggccgatgc ggtggatgat
     9481 caggctatcc tcgagcggcc agcgcgcgcg cgcctcggcc tcgatctcgg cgagcttgcg
     9541 ctcggtcatg cccgggtagt gctc
//
LOCUS       DASWHU010000426         6974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_734442, whole genome shotgun sequence.
ACCESSION   DASWHU010000426 DASWHU010000000
VERSION     DASWHU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734442"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGB88_15445"
     CDS             <1..291
                     /locus_tag="VGB88_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648900.1"
                     /translation="AQVRAWASVISVLASVLVVPAAYWIMTETVAMGRELSELRGDLK
                     SLRGAIVVTEDLRREQIGDHEARLRALERPPGRNGPYGWVPPPERDTYPRDR"
     gene            309..848
                     /locus_tag="VGB88_15450"
     CDS             309..848
                     /locus_tag="VGB88_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648901.1"
                     /translation="MGDLIMRNAKAIMVILAGAATEAVAGWLGLPVSATLEEVLTRAG
                     ELALAAGAVWWVANRPAAPKAGANVDSGDGGGAPRVPRRGAHMLLVGVALALAAAGLG
                     ACAGAIDAECQLTREQRMLNYVSLEMAAQRAGVPFLVVPFDCDLDGRPDFTPAAARAY
                     WLDRRAGTASPPDSEPPDP"
     gene            complement(1877..2854)
                     /locus_tag="VGB88_15455"
     CDS             complement(1877..2854)
                     /locus_tag="VGB88_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9648902.1"
                     /translation="MRLVRYRGVWAAAWTEAGRTVRVSLRTSDRDQAQARLHELARRP
                     VGESVGEIMAAYLADLDARAARPDRAHDAWKALGPHFAGLHPAQVDRAGCRLYARRRA
                     AQGRAPGTVGKELGILRAGLRWHDPRTPAVVELPASPAPRDRHLTRAELGALLAACRH
                     DHLRLFVVLALATAGRAGAILELTWDRVDFRRGLVKLGTAPTGQRRKGRATVPMNARL
                     RAALEAALEVRTGTAEDRVVEWAGRPVKSVKRAFAAACARAGLGPAPAKAGVTPHTLR
                     HTAAVWLAEAGTPMPEIAAFLGHDDSRTTERVYAKFSPGYLARAAKALE"
     gene            complement(3042..3593)
                     /locus_tag="VGB88_15460"
     CDS             complement(3042..3593)
                     /locus_tag="VGB88_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648903.1"
                     /translation="MRARARDEPVKGRGRCPRCACRKVRHVSHGGTPFYARNLEVCVN
                     CGALWEPLPPGGDHRDSDGTPFPFPEPCDNCAFRAGSPEQQDTPEWKKMMAKLKAGGP
                     FYCHKGVPIEPDGEHGFAYPKAHRKLRLCRGYLHMRFAKLPSAEPAPAKAGYPGEVEH
                     LCFVSDTEGAEPAPSAPPREPPS"
     gene            complement(3593..3817)
                     /locus_tag="VGB88_15465"
     CDS             complement(3593..3817)
                     /locus_tag="VGB88_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648904.1"
                     /translation="MADCIVPGCGGRGEHGRFGLCRLCWSAVPGARKIGLARRWADWR
                     SAQQPRRLADFNAARTAAIAAAGKRLAQAG"
     gene            complement(3810..4088)
                     /locus_tag="VGB88_15470"
     CDS             complement(3810..4088)
                     /locus_tag="VGB88_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648905.1"
                     /translation="MTTLSDLDAVRALCNADLAENRFGYETCVAPNCSCAGRRQAMIS
                     AVGSIMVQTMADALIAALEGVKAAARAGAADKDDGVEQPEAGRKSSHG"
     gene            complement(4085..4669)
                     /locus_tag="VGB88_15475"
     CDS             complement(4085..4669)
                     /locus_tag="VGB88_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648906.1"
                     /translation="MTATPDPGPDPESTDPARQRPKRGEVRVEARVYTPHRRHVLART
                     LDVEQANDPRYLVELTLKDLARAAADEWQGAAGAHREQVERRRARRADTKDQPQRTQS
                     AAYSAEAAASAAKAGQSTSDAPPEGESTGEDEDETSVSSVSSVVQDLESGGSGTADDG
                     ADEPPETEPAGEDERAPDLLDEIAGTLPTGESRR"
     gene            complement(4666..6411)
                     /locus_tag="VGB88_15480"
     CDS             complement(4666..6411)
                     /locus_tag="VGB88_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9648907.1"
                     /translation="MRAQARPLDDARLEALLAGHVAILGRTGSGKSYVAKGWVERLLR
                     LGRRVCVIDPTGVWWGLKSSADGKAPGFPVAVLGGDHADIALAETAGELLGRTVAERA
                     FSCVVDLSDFLIGERHRFMTDFLGALYRFNKRPLHLVCDEADEIAAQSPLPEHRRLLH
                     ATDRIVRRGRVKGFRVVLITQRPAVLHKNVLTQAAALVAMRVMAPQDRKAIEDWIKGQ
                     GDVAQGREVLDSLARLKVGEGWLWAPDLGLLERRRFPAIETFDSGRAPGNEGAAEPET
                     LAQADLAELQDLIAATSRGDAEACPGQRAGGAEQKQSPRLRVDKTALAAARAQGYADG
                     RGEAHAAAVARLDEIARLLDAVAAAAGEVGRRIAAMRAEPVPSDRAGLEIASPPQKPV
                     AATDPGSGTSQAKERHRPARGANGGDGALYSATAKLLGALARVAPARLTWTQAATLAG
                     MKARGGHFNAGRKEMRDLALVESHGDGLISIAAAGLALAGFEAPDPPRTTAERVAMWK
                     AALPALSGDILDLLVNNYPGWVPKRAVADRLGKVMRGGHWNDGMARLRNNELIESHGE
                     AIRAAPHLFTLGGTP"
     gene            complement(6408..6653)
                     /locus_tag="VGB88_15485"
     CDS             complement(6408..6653)
                     /locus_tag="VGB88_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648908.1"
                     /translation="MAKGALSEPARAVLGVLRAHGPATAAEVAARLAPVLAGGMTASA
                     AGRALLALRNRRLATFASHRTGRFRRFNAAVYMAREL"
     gene            complement(6655..6849)
                     /locus_tag="VGB88_15490"
     CDS             complement(6655..6849)
                     /locus_tag="VGB88_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648909.1"
                     /translation="MTLDEVEAEIEIAELAIVAALNALAAATGLRITGVDVGLVYATD
                     FLPGRTWSNAVRFHAELGPS"
BASE COUNT          905 a         2473 c         2581 g         1015 t
ORIGIN      
        1 gcgcaggtca gggcgtgggc gtcggtgatc tcggtgctcg cctcggtgct ggtggtgccg
       61 gccgcgtact ggatcatgac cgagaccgtc gccatgggcc gcgagcttag cgagctgcgc
      121 ggcgacttga agtcgctgcg cggtgccatc gtggtgaccg aggatctgcg ccgcgagcag
      181 atcggcgacc acgaggcccg gctgcgcgcc ctcgagcgcc cgcccgggcg caacggaccc
      241 tacggatggg tcccgccgcc cgagcgcgac acctatcccc gcgaccgtta gcagcgaagg
      301 agacgcccat gggcgacctg atcatgcgca acgccaaggc gatcatggtg atcctcgccg
      361 gcgccgcgac cgaggcggtg gccggctggc tgggcctgcc ggtctcggcg acgctcgagg
      421 aggtgctgac ccgggccggc gagctcgcgc tcgccgccgg cgccgtgtgg tgggtcgcga
      481 accgcccggc ggcgccgaag gctggcgcga acgtcgactc gggcgacggg ggcggcgcgc
      541 cgcgcgtgcc gcgccggggc gcccacatgc tactggtggg tgtggcgctc gccctggcgg
      601 cggccgggct cggcgcctgc gccggcgcga tcgacgcgga gtgccagctt acccgcgagc
      661 agcggatgct caactacgtc tcgctcgaga tggccgcgca gcgcgccggc gtgccgttcc
      721 tggtggtgcc gttcgattgc gacctcgacg gccggcccga cttcaccccg gccgcggcgc
      781 gcgcctactg gctcgaccgc cgggccggca ccgcctcgcc gcccgactcc gagccgcccg
      841 atccctgatc ctgacgtccg ttcctgatcg tcgcccccgg ctaacggccg ggataagacg
      901 cggtttcacg ctttcacccc tagtgtgaaa gccggtgact ccagatcggg tttccgcgaa
      961 accagttgac catagcaacg gccctctcgg cgtgcgccga gggggccggg ctttatgcgt
     1021 ttcggatgtc ccgttttctg ccctcaagcg aggagggaaa acgggacatg gcagcggcag
     1081 ccggacatcg ttcacccggt tccttccgct gtcaggatgg ctcgcggtgg gccgtcaagg
     1141 gaaatgagtt cgcaccggac aagagctgga tcacgaaacc agggcctcgg ccctgccata
     1201 tctccttcca gaacaacggc caattcgcgg gcttccgact cggttcgggc gaggatgacg
     1261 cacccgtaga tttcggagtg gtcataccac tcgatacctg cggcatgcat ggcgtcctga
     1321 tcgggcctca gaagaaacac cttgagcggc gtctcggttt ggttcatttg caccaacgcg
     1381 ccctgtcctc caccacccct aagtgattga aaaccctggt gggcgcggct gggattgaac
     1441 cagcgacccc cgccgtgtga agacctttcc ctccacgatc tagcggtgca gggcgcgcca
     1501 gatatgccag atgtggtgca ccgccggtca gaaagagccc ccggcggcag gagtttggtt
     1561 cacttgcacc aaagggaact gtcctacgct ctaatcgaca tcgtcatcag gaaggtcttg
     1621 ccacgccacc ggctcgactc ggattgcaaa acacgctgcc cagcccccac tatccgggtc
     1681 gccttgctca actgcgcaga tttcccagtc cagtttctcc agggtcgggg cgtaccagcc
     1741 tattacgacg cggcagcggt taagcggccc cgcttcaacc ggatcgcaca gcagagccag
     1801 cggcttgctc aacctgagaa ggattgggcg gccgtcgcga ggtgctgacg acatcgactg
     1861 ccaagtcggc atcgctctac tccagcgcct tcgccgcgcg ggcgaggtag ccgggggaga
     1921 acttggcgta gacgcgctcg gtcgtgcgcg aatcgtcgtg gccgaggaag gcggcgatct
     1981 cgggcatcgg cgtgccggcc tcggcgagcc acaccgcggc ggtgtggcgg agcgtgtggg
     2041 gggtgacccc cgctttcgcg ggggcaggcc cgaggccagc gcgcgcgcag gccgcggcga
     2101 aggcgcgctt gaccgacttg accggccggc ccgcccactc gacgacgcgg tcctcggcgg
     2161 tgccggtgcg cacctcgagc gcggcctcga gcgcggcgcg caagcgcgcg ttcatcggca
     2221 cggtggcacg gcccttgcgc cgctgccccg tgggcgcggt gccgagcttg accaggccgc
     2281 ggcggaagtc gacccggtcc caggtcagct cgaggatggc gccggcgcgc ccggcggtgg
     2341 ccagcgccag gaccacgaac agcctgagat ggtcgtggcg gcacgccgcc agcagcgcgc
     2401 cgagctcggc cctggtcagg tggcggtcgc gcggcgccgg cgaggccggc agctcgacca
     2461 cggccggggt gcgcgggtcg tgccagcgca agccggcgcg caggatgccg agctccttgc
     2521 cgacggtgcc gggcgcacgc ccctgcgcgg cgcgccggcg ggcgtagaga cggcaacccg
     2581 cgcggtcgac ctgggccggg tgcaggccgg cgaagtgggg cccgagcgcc ttccaggcgt
     2641 cgtgggcgcg gtcggggcgg gcggcgcgcg cgtcgaggtc ggcgaggtag gcggccatga
     2701 tctcgcccac gctctcgcct accggccggc gcgcgagctc gtggagacga gcctgggcct
     2761 ggtcccgatc actggtcctg agcgagaccc ggacggtgcg cccggcctcg gtccaggcgg
     2821 cggcccagac gccgcggtat ctgacgagcc gcatagcaac ctctcggcct catggtgggg
     2881 gatgcgcacc ggcgagcgca aggggacggt ctggatcagg ccctccctga tccggcgccg
     2941 gacggtcgac ggcgacaccg cgagcgcctc ggccaggcgt gcgaccgtcc aggcgcgggg
     3001 gagggcgcct gccccggact gcgatccggg ggcggtggtg gtcatgacgg gggctcacgc
     3061 ggaggcgcgg agggcgcggg ctcggcgcct tccgtatcgg agacgaagca caggtgctcg
     3121 acctcgcccg gataccccgc tttcgcgggg gcaggctccg cgctcggcag cttggcgaaa
     3181 cgcatgtgca gatagccgcg gcacaggcgc agcttgcggt gggccttggg ataggcgaag
     3241 ccgtgctcgc cgtccggctc gatcggcacg cccttgtggc agtagaatgg cccgccggcc
     3301 ttgagcttgg ccatcatctt cttccactcg ggcgtgtctt gctgctcggg cgacccggcg
     3361 cggaaggcgc agttgtcgca cggctccggg aacgggaacg gcgtgccgtc gctgtcgcga
     3421 tggtcaccac ctggcggcag cggctcccac agcgccccgc agttcacgca cacctcgagg
     3481 ttgcgggcgt agaacggcgt gccgccatgg ctgacgtggc gcaccttgcg gcacgcgcag
     3541 cgcgggcagc gcccgcggcc cttgaccggc tcgtcgcggg cccgtgcgcg catcacccgg
     3601 cctgtgccaa ccgcttgccc gccgcggcga tggcggcggt gcgtgcggcg ttgaaatcgg
     3661 ccaggcggcg cggctgttgc gcgctgcgcc agtctgccca gcgccgggcg aggccgatct
     3721 tgcgcgcgcc cggcaccgcg ctccagcaca gccggcacag gccgaaccgg ccgtgctcgc
     3781 cgcggccgcc gcagcccggc acgatgcaat cagccatggg atgacttacg ccccgcctcc
     3841 ggctgctcga ccccatcatc cttatccgcg gcgccggcgc gcgccgcggc cttgacaccc
     3901 tccagtgccg cgatcagggc atcggccatg gtctggacca tgatcgaccc cacggcagag
     3961 atcatcgcct ggcggcggcc ggcgcacgag cagttcggcg cgacgcacgt ctcgtagccg
     4021 aaccggttct cggccaggtc ggcgttgcac agcgcgcgca ccgcgtcgag gtcggagagg
     4081 gtggtcatcg cctggactcc ccggtcggca gggtgccggc gatctcgtcg agcaggtcgg
     4141 gcgcgcgctc gtcctcgccg gccggttcgg tctcgggcgg ctcgtcggcc ccgtcgtcgg
     4201 cggtgccgct cccgccggac tccaggtctt gaaccacaga ggacacagag gacaccgacg
     4261 tctcgtcctc gtcctcgccc gtggactcgc cttccggggg tgcatcgctg gtgctctgtc
     4321 cggccttcgc agccgaagcg gctgcttcgg cggagtaggc tgcactctgt gtcctctgtg
     4381 gttgatcctt cgtatctgcg cgcctggcgc ggcggcgctc gacctgctcg cggtgggcgc
     4441 cggccgcgcc ctgccactcg tcggcggcgg cgcgggcgag gtccttgagg gtcagctcga
     4501 ccaggtagcg cgggtcgttg gcctgctcga catcgagcgt gcgcgccagc acgtggcggc
     4561 ggtggggcgt gtagacccgc gcctcgacgc ggacctcgcc gcgcttgggg cgctggcggg
     4621 ccgggtcggt gctctccgga tcgggcccgg ggtcgggagt cgcggtcatg gcgttcctcc
     4681 aagggtgaac aggtgcggcg cggcgcggat tgcctcgccg tggctctcga tcagctcgtt
     4741 gttgcgcagc cgtgccatgc cgtcattcca gtggccgcca cgcatgacct tgccgaggcg
     4801 gtcggcgacg gcgcgcttcg gcacccagcc cgggtagttg ttgaccagca ggtcgaggat
     4861 gtcgccggac aaggccggca gcgcggcctt ccacatcgcg acgcgctcgg cggtggtgcg
     4921 cggcgggtcc ggcgcctcga agccggcgag cgcgaggccg gcggcggcga tcgagatcag
     4981 gccgtcgccg tggctctcga ccagggcgag atcgcgcatc tccttgcggc cggcgttgaa
     5041 gtggccaccc cgcgccttca tgccggccaa ggtcgcggcc tgggtccagg tgagcctggc
     5101 cggcgcgacg cgcgcgagcg cgccgagcag cttggcggtc gcgctgtaga gcgctccgtc
     5161 gccgccgttc gcgccgcgcg cgggccgatg ccgttccttc gcctgcgagg tgccgctgcc
     5221 cggatcggtt gcggcgaccg gcttttgcgg cggggaggca atttccaggc cagccctatc
     5281 tgaggggacc ggctcggcgc gcatcgctgc gatccgccgg ccgacctcgc cggccgcggc
     5341 cgccacggca tcgagcagcc gtgcgatctc gtcgagccgg gcgaccgcgg cggcgtgggc
     5401 ctcgccgcgg ccgtccgcat agccttgtgc gcgggccgcg gccagcgccg tcttgtccac
     5461 gcggagacgc ggagattgct tctgctccgc gcccccggct cgttggccgg ggcaggcctc
     5521 cgcgtctccg cgtgacgtcg ccgcgatcag gtcctggagc tcggcgaggt cggcctgggc
     5581 cagcgtctcg ggctcggccg cgccctcgtt gcccggcgcg cggcccgagt cgaaggtctc
     5641 gatggcgggg aagcggcggc gctccaagag gccgaggtcg ggcgcccaca gccagccctc
     5701 gccgaccttg aggcgggcga gcgagtcgag cacctcgcgg ccctgggcga catcgccctg
     5761 gcccttgatc cagtcctcga tcgccttgcg gtcctgcggc gccatgaccc gcatcgcgac
     5821 cagggctgcg gcctgggtga gcacgttctt gtgcaggacc gccgggcgct gggtgatcag
     5881 caccacccgg aagcccttga cccggccgcg gcggacgatg cggtcggtgg cgtggagcag
     5941 ccggcggtgc tcgggcaacg ggctttgggc cgcgatctcg tcggcctcgt cgcagaccag
     6001 gtgcagcggg cgcttgttga agcggtagag cgcgcccagg aagtcggtca tgaaccggtg
     6061 gcgctcgccg atcaggaagt cgctcaggtc gaccacgcac gagaatgcgc gctcggcgac
     6121 ggtgcggccc agcagctcgc cggcggtctc ggcgagcgcg atgtcggcgt ggtcgccgcc
     6181 gagcaccgcg accgggaagc ccggcgcctt gccgtcggcg ctcgatttca gaccccacca
     6241 caccccggtc gggtcgatca cgcacacccg gcggccgagc cgcagcagcc gttccaccca
     6301 gcccttggcg acgtaggact tgcccgaccc ggtgcggccc aggatggcga cgtggccggc
     6361 gagcagggcc tcgagccggg catcgtcgag cggacgcgcc tgcgccctca tagctcgcgc
     6421 gccatgtaca cggccgcgtt gaagcgccgg aagcggccgg tgcggtggct ggcgaaggtg
     6481 gccaggcggc ggttgcgcag cgccaacagg gcgcgcccgg cggcggacgc cgtcatgccg
     6541 ccggccagca ccggggcgag ccgcgcggcg acctcggcgg cggtggcggg gccgtgcgcg
     6601 cgcagcacgc cgagcacggc gcgcgcgggc tcgctcaatg cgcccttggc cacgtcagga
     6661 cggccccaac tccgcatgga agcgcaccgc attgctccag gtccgccccg gcaggaaatc
     6721 ggtggcatag acgagaccga catcgacccc ggtgatgcgc aacccggtcg ctgcggcgag
     6781 cgcgttcagc gcggcgacga ttgcgagttc ggcgatctcg atttccgcct ccacctcgtc
     6841 gagggtcatg ccggccacgg cgtgtcggat ggcggctcgg ggtgatcgcc tcgcctaagc
     6901 tctatggcgt cgcccgtccg ggtagtcagc ctaagcgtat cgtcgaagcc gttccggtag
     6961 gcgcgcagcg cggc
//
LOCUS       DASWHU010000427         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate SMAG_U2850
            k141_737521, whole genome shotgun sequence.
ACCESSION   DASWHU010000427 DASWHU010000000
VERSION     DASWHU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960497
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (soil metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:19:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 3,064
            CDSs (with protein)               :: 3,064
            Genes (RNA)                       :: 22
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737521"
                     /isolate="SMAG_U2850"
                     /isolation_source="soil"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1860
                     /locus_tag="VGB88_15495"
     CDS             <1..>1860
                     /locus_tag="VGB88_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9648910.1"
                     /translation="AALGVRLRFEREVPDPSRLAGSDLIVAADGINSAVREQYADHFQ
                     PRIEWRGNKFIWLGSTCRFPAFTFIFRENRHGLWRVHAYQFDREQSTLIFECTEDTWR
                     RAGLDQADEARSIAYLEDLLADHLDGHRLIENRSTWRNFPIVTNDRWHHDNIVLLGDA
                     VHSAHFSIGSGTKLAMEDATALVGALDAHATIPDALAAYEAERRPVVESTQRAAKVSL
                     MWFENVERYYHELETIQFAFSLLTRSLRISHENLKLRDPAFVRRVDAWFAEKAANQAR
                     VNVAADPPPPPMFTPFRLRDMVLANRVVVSPMCQYSAEDGTVDDWHLVHLGSRAIGGA
                     GLVITEMTDTSRDGRISPGCAGMYQPRHVGAWARVVDFVHRYSHARIGLQLAHAGRKG
                     STRRLWEGMDEPLEGGNWPLIAASPIAYAPANQVPKAMDRADMDRVRCDFVRAAEMAI
                     EAGFDLLEVHFAHGYLLSTFLSPLTNARKDSYGGSLENRMRYPLEVFDAVRAAWPAER
                     PMSVRISATDWAPGGFTAEDAVGLGRALKAHGCDIVDVSAGQVVSWQEPEYGRLFQTP
                     FSDQVRLEADVATMTVGNISSYEDVNSILAAGRADLCVLARAHLYDPYWTRHAA"
BASE COUNT          280 a          679 c          621 g          280 t
ORIGIN      
        1 gccgcgctcg gcgtgcgcct gcgcttcgag cgggaggtgc ccgacccctc gcggctcgcc
       61 ggatcggacc tgattgtcgc cgccgacggc atcaacagcg cggtgcgcga gcagtacgcc
      121 gaccacttcc agccgcgcat cgaatggcgc ggcaacaagt tcatctggct cggcagcacc
      181 tgccgcttcc cggccttcac cttcatcttc cgcgagaacc ggcacgggct gtggcgggtg
      241 catgcctatc agttcgaccg cgagcaatcg accttgatct tcgagtgcac ggaagacacc
      301 tggcggcgcg ccggccttga tcaggccgac gaggcaagga gcatcgccta tctggaggac
      361 ctgctcgccg accacctgga cggccaccgc ctgatcgaga accgctcgac ctggcgcaac
      421 ttcccgatcg tcaccaacga ccgttggcac cacgacaaca tcgtgctgct gggcgatgcg
      481 gtgcacagcg cccacttctc gatcggctcg ggcaccaagc tcgccatgga ggacgcgacc
      541 gcgctggtcg gcgcgctcga cgcgcacgcg acaattccgg acgcgcttgc cgcctacgag
      601 gccgagcgcc ggccggtggt cgagagcacc cagcgcgcgg ccaaggtgag cctgatgtgg
      661 ttcgagaacg tcgagcgcta ctaccacgag ctcgagacca tccagttcgc gttctcgctg
      721 ctcacccgtt cgctcaggat cagccacgag aacctgaagc tgcgcgatcc cgcgttcgtg
      781 cgccgggtcg atgcgtggtt cgccgagaag gcggccaacc aggcgcgcgt caacgtcgcc
      841 gccgacccgc cgccgccgcc gatgttcacg cctttccggc tgcgcgatat ggtgctcgcc
      901 aaccgggtgg tggtctcgcc catgtgccag tactcggcgg aggacggcac tgtcgacgac
      961 tggcacctgg tccatctcgg cagccgcgcc atcggcggcg ccgggctggt gatcaccgag
     1021 atgaccgaca ccagccgcga cgggcgcatc agccccggct gtgccggcat gtaccagccc
     1081 cggcatgtcg gcgcgtgggc ccgggtggtc gacttcgtcc accgctacag ccacgcccgc
     1141 atcggcctgc agctcgccca tgccgggcgc aagggctcga ccaggcggct gtgggagggc
     1201 atggacgagc cgctggaggg cggcaactgg ccgctgatcg ccgcctcgcc gattgcttac
     1261 gcgcccgcca accaggtgcc gaaggcgatg gaccgtgccg acatggaccg cgtcaggtgc
     1321 gacttcgtgc gcgccgccga gatggcgatc gaggccggct tcgacctgct cgaggtccac
     1381 ttcgcccacg gctacctgct gtcgacgttc ctgtcgccgc tgaccaatgc gcgcaaggat
     1441 tcctacggcg gcagcctgga gaaccgcatg cgctacccgc tcgaggtgtt cgacgcggtg
     1501 cgcgccgcct ggcccgcgga gcggccgatg tcggtgcgca tctcggccac cgactgggcg
     1561 ccgggcgggt tcaccgccga ggacgccgtc ggcctggggc gggcgctcaa ggcgcacggc
     1621 tgcgacatcg tcgacgtctc cgccggccag gtggtgtcgt ggcaggagcc cgagtacggg
     1681 cgcctgttcc agaccccgtt cagcgaccag gtgcggctcg aggccgacgt ggcgaccatg
     1741 accgtcggca acatctcgtc ctacgaggac gtcaactcga tcctcgccgc cgggcgcgcc
     1801 gacctgtgcg tgctcgcccg cgcccacctg tacgatcctt actggacccg ccacgccgcg
//
